LOCUS       DAOQLC010000001       125787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_11_length_125787_cov_23.144037, whole genome shotgun sequence.
ACCESSION   DAOQLC010000001 DAOQLC010000000
VERSION     DAOQLC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 125787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 125787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..125787
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11_length_125787_cov_23.144037"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PL000_00005"
     CDS             complement(<1..809)
                     /locus_tag="PL000_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC87302.1"
                     /translation="MDFNQFQLDSRLMQGIQKAGYEKATPIQEAAIPAALRGRDIIGT
                     AQTGTGKTAAFVLPILNKLLSGQRNTARALIVTPTRELAEQINDVIKVLSAGTKLKSA
                     TIYGGVGANPQIQALRNGAEILVACPGRLLDLIAQGHAKMANIEFLVLDEADRMFDMG
                     FLPDVRRIVKAVPEKRQTMLFSATFPSDVELLAQQALKQPQKIAMGIIKPAHTVTHAL
                     YPVAPHLKSTLLLELLKRTDTNSVLVFTRTKYRAQKVAQQIHKAGLKVTSL"
     gene            complement(1039..1935)
                     /locus_tag="PL000_00010"
     CDS             complement(1039..1935)
                     /locus_tag="PL000_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC87303.1"
                     /translation="MNFIGEIAGLATSFFFAITAIIFASVGRMVGSQVTNRFRLLFAL
                     FYLVILNLIFFHEPLPFSAGSPRWLWLSLSGVIGLSLGDAFLFRSYVAVGPRLGSLLL
                     SLAPIFGSVIAWIFFGETLTALQILGILLALAGIGWVVTSHKEEANTPKGHTREGIIF
                     GILAGLGQAVGLVLSKQAMAGGFSPFQANAIRMLAAVIFTWAWTAVEGQISSTVETIR
                     NHPKALQWIAFGAVIGPVLGVSSSLLAVQHAEIGVASTLMALPPVIVLPISYFVFKEK
                     IGWQGIAGTFLAILGVAVLFLA"
     gene            complement(1939..2820)
                     /locus_tag="PL000_00015"
     CDS             complement(1939..2820)
                     /locus_tag="PL000_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC87304.1"
                     /translation="MRKLIGILLIAISAASFGTLAIFGRYAYENGMDTFTVLFLRFGI
                     SASFMTVILLLRKEHLPRGKILAQLIGMGALGYVGQSFMYMTAINYASAGLVALLLYL
                     YPFFVAVLSMIFLHESLTRVKIIALVLALFGTALTVGPVSGQIIGALMAITAALIYSI
                     YIIVGTNVMKHVTAVQSSLVIFASAGAVFGILAFSKGTHFPVNNMGWFAMLGIIVIST
                     IIPVVTFLAGLERIGPTNAAMLSTLEPIVTVLLAAWLFGDKLLPIVMLGGGLILVAVM
                     LLTRAELGKDSGNQPGV"
     gene            complement(2876..3466)
                     /locus_tag="PL000_00020"
     CDS             complement(2876..3466)
                     /locus_tag="PL000_00020"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNC87305.1"
                     /translation="MIHTYQINGLTLQTGDIICTMNGKPDILPGEFWRLIGRLVPGDV
                     DHVAIYLGPDGRCAEAGARGVITFDVPNGHWDTERMARQRGLLFDTFYGIAAPLDVLG
                     FSDEDEQLMREAVADYVLAQLGKPYNLNFLNPETEETFYCSQLAYKAYQKIGIDLNTG
                     LAMEQLPGTNQIIYPQEIWDGFSHRQSEARQETISR"
     gene            complement(3513..4562)
                     /locus_tag="PL000_00025"
     CDS             complement(3513..4562)
                     /locus_tag="PL000_00025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC87306.1"
                     /translation="MKIGLVIYGSIDTLSGGYMYDRMLVEYLRAQGDSVEIISLPWRN
                     YAAHLTDNLTFKLPPNLDILIQDELNHPSLIGANHGRHPYPIISLVHHLRCSELRPKW
                     QNDLYRPIEKRYLQSVDGFIFNSKTTKGVVNDLIGSGKPDVVAYPPTDRFGDAISEEE
                     IKERSKKKELHMLFLGNVMGRKGLHTLLEAVRLSNLDLRLDIVGSLATEPEYAAQMQE
                     FVSTNGLSSAVHFHGALNNQPLVERLKAAHILVVPSSYEGFGIVYLEGMGFGLPAIGT
                     TLGAAGEVIEEGVTGYLIQPGDSHALAARLQLMNEKRDLLIQMSLAARNRYLVQPKWN
                     QTAHQIREFLLSFSG"
     gene            complement(4614..5000)
                     /locus_tag="PL000_00030"
     CDS             complement(4614..5000)
                     /locus_tag="PL000_00030"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNC87307.1"
                     /translation="MYTLGVRREFIARHFLIGGDWGPENFPNSHHYILELQLKGSELD
                     QHGYLVDIVDVEKHLDDVVGYYKEQMLNEKPEFAGLNPSIEHFSRILAETLNERIKAR
                     NISKLKVVLWEHENAWAAFSVERLAR"
     gene            complement(5028..5714)
                     /locus_tag="PL000_00035"
     CDS             complement(5028..5714)
                     /locus_tag="PL000_00035"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNC87308.1"
                     /translation="MKFNYTTLETCHTATGIVLVIDVLRAFSTAAYAFSRGAREIRLV
                     SAVEEALELKAQLPNSKVMGEVGGLPPAGFDFGNSPTRILEHDLTGITLIQRTGAGTQ
                     GAVRSVHAEVMFAASFVVAQATMEHVLRLRPEEVTFVVTGGADNAEDLACAEFFEKQF
                     TGQAVDSEELIRRVVQSRDAFQHMNDHPQFPRSDLDLCTRINAFNFAMPIERKNGFLI
                     MQALNLKPEN"
     gene            complement(5711..6721)
                     /locus_tag="PL000_00040"
     CDS             complement(5711..6721)
                     /locus_tag="PL000_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HNC87309.1"
                     /translation="MPKAQTIFFTAPKQVEIKEITLPPLKKDEVLVETICSAISAGTE
                     MLVYRGQFPHLADSHDTFSSDLNYPLAYGYACVGKVKEIGADVSRAWKDKHVFAFHPH
                     ASSFIINPSSLIPIPDSLTSENACFLPNMETAVNLVQDAAPILGERVLVLGQGVVGLL
                     TSSLLSEFPLESLLTADFYELRRKAAMQIKGVHSLDSSLKTFHVDSIAYTQNKFDLTL
                     ELSGSPSALNDAIELTIFSGRIVIGSWYGQKRAEIDFGGSFHRSRIRLISSQVSTISP
                     DLSGRWDKSRRFDVAWHALERIRPEKWITQRFSIADAAKAYQLLDEKPQETIQVVFDY
                     LK"
     gene            complement(6784..7170)
                     /locus_tag="PL000_00045"
     CDS             complement(6784..7170)
                     /locus_tag="PL000_00045"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC87310.1"
                     /translation="MQERRKQQRKNLMAYTQVFDLNGGHLLGYLGDLNLHGAMVIGEK
                     PIAENTELTLAIELPELENVKIPRITIPARAAWCQQDISPEYFNVGFEFKEVTPKQKP
                     VIEEIMEKYEFRRDLPSYLTKPVPNK"
     gene            7258..7983
                     /locus_tag="PL000_00050"
     CDS             7258..7983
                     /locus_tag="PL000_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC87311.1"
                     /translation="MRLIYISPHLDDAILSAGGLIHDQVQAGRHVEIWTVFCGFPPAN
                     QLSSFAQIIHQKWGIMDTNELVAARRAEDKAAADIAGAQVFHFDFLDCIYRRGRDGNW
                     LYSDVFIPPHTDDDGLPAQIAEAISARLNPDDELVCQLALGAHLDHTLVRRAAELLGR
                     PLTYDVDIPYLFNFPDDFALKTAKMKEITHRITEAGLRSWGDAILAYTSQFGTLFSSP
                     EEMREKLQQYWAEKQGISFWKFE"
     gene            8124..8600
                     /gene="greA"
                     /locus_tag="PL000_00055"
     CDS             8124..8600
                     /gene="greA"
                     /locus_tag="PL000_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC87312.1"
                     /translation="MPTTNFLTKEGFQKLQEELDHLRTVKRQEVADRLHEAMEGGELI
                     ENAEYEAAKNEQAFVEGRIQELEILLATANIIEENNGKAKKNDLIHVGSKVTIKEGNF
                     EAETFTIVGAAEANPREGKISNESPIGKAILNHKLGDIVKVETPGGTYNVKIIKVG"
     gene            8703..10235
                     /gene="lysS"
                     /locus_tag="PL000_00060"
     CDS             8703..10235
                     /gene="lysS"
                     /locus_tag="PL000_00060"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNC87313.1"
                     /translation="MAEYNSLEKIRLQKIEELRAEGIESYPTRAERTHTNAKVIAEFE
                     AAEKNAAPDTTPEVKAVLVGRIRANRAMGKVSFAHIEDGSGKIQLFFRSNELGKERLD
                     LFNKMFDLGDFIQASGVMFRTKTGEVSLHVHDFKLLAKSVTPLPADKDVTQEDGTVTR
                     YAALESPELRARQRYADLAVNPDVRETFRKRATLIKALRNFLDGHDFLEVETPILQPL
                     YGGAAARPFVTHHNELDQDMYLRISFELYLKRLLVGNLERVYEIGRDFRNEGVSFKHN
                     PEFTQLEFYWAYADYLQVMELTEQMVAYVAEQVTGSTKVTFGEYELEFKPPWNRVEMR
                     QGILETSGIDIAEHKTAEELFKAIRAKHPNKTPDPKSARGKMIDFLLGEFLEPTLIQP
                     TFLYNYPRDISPLAKSIPGDPLTVERFEGYAAGFELCNAFTELNDPLDQEQRFIEMGR
                     DYTDDEEEKHPLDEDYLRAMRYGMPPNGGFGMGVDRLAMLLTNKHSIREVLLFPALRK
                     EE"
     gene            10243..10902
                     /locus_tag="PL000_00065"
     CDS             10243..10902
                     /locus_tag="PL000_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87314.1"
                     /translation="MHYISPDKEEQIRQIARSGNLIEAIKEYRLLTNASLKDAKSAVE
                     AMVRGNYVNGPAPQAVANGADSSQKSQILDLMSKGREIEAIKLYRGWTKAGLKEAKDA
                     VEAMARGEDVQIPEPVTSVESGSMDGQIRALLAKNQKIEAIKVHRAATNSSLKEAKDY
                     VEAIERGVRPNPSSPLNIPLSNDPFEEENVRTRRMMVFVFIILAILAVVFAFAFLRGG
                     L"
     gene            10911..11297
                     /locus_tag="PL000_00070"
     CDS             10911..11297
                     /locus_tag="PL000_00070"
                     /inference="COORDINATES: protein motif:HMM:NF022700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HNC87315.1"
                     /translation="MQKSKLVLIFGDVIAIALLTFIGFATHGEADVSFLPRMAAAFFP
                     VLIGWFVLAPWFGLFDEQIISNPRSLWRILPAMLAVAPLAVMLRAAILNAAALPLFTL
                     VLGGTNAFGMLVWRALHLFVVKRNAR"
     gene            11342..11950
                     /locus_tag="PL000_00075"
     CDS             11342..11950
                     /locus_tag="PL000_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC87316.1"
                     /translation="MDEPALIQTAQNGDLDSFNTLILHYQDRVYNTALRILGDEDQAA
                     DAAQEAFISAFRSINSFRGGSFKAWLMRTVTNACYDELRRQKRRPTTPLEPDTADGEE
                     MDSPRWLADPNMTPAEKSEADELEHAIQHCLDALPTDFRTVVVMADLQGLDYTEVAAA
                     VRVPLGTIKSRLARARLRLRECLRGFEELLPSSFRLEEESRL"
     gene            11947..12732
                     /locus_tag="PL000_00080"
     CDS             11947..12732
                     /locus_tag="PL000_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87317.1"
                     /translation="MKNFRDIEQLSSYLDGQLSPSDSARVESRIASDPELASAFEDLR
                     ATRGILRKLPARKAPRNFTLTRQMVGLKPPVSRSYSFFRFSSAFATVLLMLTFAANYV
                     APRMSVVPVGGSGGGCDPCGLGGGGGPESFNMDALPAATEAPAVAAESFLATEAPAAT
                     EAPAMVEEPAATEAPVEVLPTMDMALAPTELPLSTQDTQRIVETPSASPKEPVIESAP
                     QQQPPVEDESFISATWQVVLLVIGLVSGLAAFFMNRSAKKKWS"
     gene            12736..13221
                     /locus_tag="PL000_00085"
     CDS             12736..13221
                     /locus_tag="PL000_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC87318.1"
                     /translation="MARDSHIKFCPRCGAEVAHEEKFGMVRPICPQCGWIYFIDPKVA
                     AAILVEQEGRVLLVRRVNEPFRGLWTLPAGFVNGGEDPAKAAARECLEETGLHVHVTG
                     VLDVIAGREHERGADFIIVYHAEVTGGELVPADDADAAEWFLRSELPPLAFNATKKIL
                     E"
     gene            13244..13423
                     /locus_tag="PL000_00090"
     CDS             13244..13423
                     /locus_tag="PL000_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87319.1"
                     /translation="MGEKLDNKKDFGVLSPKSFLLRMILLLIKPLFSAAGTPPSALFI
                     STDYLAQKSFYGAFW"
     gene            complement(13405..15564)
                     /locus_tag="PL000_00095"
     CDS             complement(13405..15564)
                     /locus_tag="PL000_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNC87320.1"
                     /translation="MGNTTSKIGIPVIIAAVVICIACVCCAGVLLYIYGDSIFGGTSV
                     TTPPVVSTDPIIPADTTNLPEWTIIYYSDADDDILEGDLWFDINEMELVGSNTQMNIV
                     VQIDRAEGSFDGDGDWTDARRLLITQDSDLNQINSPVVQNLGEVDMGNPQTLVDFMTW
                     TIQNYPAKKYALIMSDHGGGWTGGFSDLQSGSSLSMPQIVQAIEVVQQQMGGQKFEII
                     GFDACLMGMVEVYGSLYPYSNYMIASEEVIPSTGWSYAAWMGQLAQNPSMDGRGVSQA
                     IISTYITQDTLLTLRSTSSSEIEEVEAATTLSAVESARIPDVIGAMNQFISVMATIDQ
                     RYVAQAREYTRSYYSIFGEDTPSPFIDLGNFSEILANATGDPAIFQASEQLKAAISSA
                     VVAEKHGQRMSGSNGISFHFPISDIYAYTEYNSDFPPYYAESAARFLQLSTWDEFLAY
                     HYTGQEFTPQEGQAYTPPRTNEINAPGASPLTIAPIQLSDTVISADETLTLSTTVSGD
                     VSYIYFVLFFYNQDANVTWLADMSFVSAPNTISVDGVNAPDYGESPIEISYEWEPYLF
                     VLKDGTNQAYALFEPDEYESADGVTTYSVYGQYTPVNATTPTDAKLTFDLDGNLLNIY
                     ALPDSDSNGIDTAVAISPQIGDRFTDYVQYYTFDANGVATYTNELSDDVFTYGEQGFW
                     FESYIPVDGQYSIGFYAVDFDNNITSSYEYITYQNAP"
     gene            15734..16408
                     /locus_tag="PL000_00100"
     CDS             15734..16408
                     /locus_tag="PL000_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87321.1"
                     /translation="MKKQYRMLFAFAALLLLVSIACNGGAAPTAAPVPTLPPAPTSQP
                     QSNNPPSNNNPIVPQPPALNEPPPSNGNSSDLVVFTDENNLLSFEVPGDWYYEQQTYE
                     HAYIDRITSTDGSAIIDSLVYNDGQAFTGNDNGRFALQLLHQYYSNTGKEGDISISGD
                     EMQPDGSEKLTWTSKSGGYSGVSFFELRGGDRMTFLMFTLYYANDADQAVIDILDHAI
                     TSYTIP"
     gene            complement(16477..17241)
                     /locus_tag="PL000_00105"
     CDS             complement(16477..17241)
                     /locus_tag="PL000_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87322.1"
                     /translation="MKTSKNLSSVLAISSLILAMLACNFGKADATPTPLPPTNPPPTA
                     IPPTEAPPVVEASPTPAEVVPSVDTSAFETQLQEFADEGYVSTTEGEITPIDPFEEEW
                     AQLNYYQWWTIDKEASDLVFSGHFKWSSSNSTPDLSGCGVVFGLQEGSDDHYVVFLDK
                     GRIAFFMSRGSSVYEVGKTRGPGRVNFGNPAEADFSLAVRGKSAFVSVDGEVTEYTLS
                     ADQGTNGQFALTLLSGTNSGYGTRCEMTDMFLFIPE"
     gene            17406..18248
                     /gene="amrB"
                     /locus_tag="PL000_00110"
     CDS             17406..18248
                     /gene="amrB"
                     /locus_tag="PL000_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNC87323.1"
                     /translation="MTTLDLRPSPIAGQWYDANPTALARSVDDFLAHASVPQLNGEVL
                     ALIAPHAGHLYSGAVAAHAFATLHGLAPELVAVLSPYHNYAHFPLITTKHQAYFTPLG
                     NLSVDQAALAELQTHLEIPITAIANDREHSLEIELPFLQRVLKNEFKLLPIMIRAQDV
                     TIAQKLGHALAQTLKGKNAIIVASTDLSHFYNQKTAGILDAEMLRRFESFDPESVFDA
                     EQTGVGFACGHAAVASMLYAARELGADKVQILKYATSGDVTGDTSSVVGYGAGVVLRP
                     STSH"
     gene            complement(18278..18712)
                     /locus_tag="PL000_00115"
     CDS             complement(18278..18712)
                     /locus_tag="PL000_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87324.1"
                     /translation="MLTIIMILGGIAILASVILLAFMFIKSNEVNLTGKTEDKPEWMK
                     SNPPKETVKATRAEGEGVTLFDHDAGEKIASPFAEQIEDILRAKLESDPFNKFDIDFG
                     SAPDGSLEIWVNGSMYQSVDGLPDEGLKNAFRNAVKEWERLR"
     gene            18754..21189
                     /gene="priA"
                     /locus_tag="PL000_00120"
     CDS             18754..21189
                     /gene="priA"
                     /locus_tag="PL000_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC87325.1"
                     /translation="MFARVAVNVSVISGLFDYAIPNELALQIQAGSFVIVPFGKQTVQ
                     GIVTALIDTPAVENPKPIMELLDPAPVLTQPQLALAIQLAESTLNPLAAIISLMLPVG
                     LSQQADVHYELRNANYEDKTSTVATRLLTLLRERGPLRGRQIDSHFSRVDWRKTAQAL
                     VRKGILATKHVLPPPRVRSKFVRVAQLSVTPEAAEAEMDSLGTKQTLARRQSALRYLI
                     QQPEAINLSWVYAETGCNLADLQELEERGLIRLFENEIFRDSLQRIENGKEEHGRQFE
                     LTSEQSSSLEKITHSSFILQPSPFLLHGVTGSGKTEIYLRAAEETIKRGKQAIILVPE
                     IALTPQAVRRFLARFPGQVGLIHSKLSEGERYDTWRRARSGKLKVIIGARSALFAPLP
                     NIGLIVVDECHDSSYHQTEPPFYNAAVAAQEYARLCGAVCVLGSATPTVEQRFAAENG
                     TLTKLTLPNRVTDSALPPVQVVDMREELKTGNRGIFSRALAESLAETISRGEQAILFL
                     NRRGTATYIFCRDCGFVLKCPNCDTPLTLHVGTFERSNVPTLLCHHCGYTRQKPKTCP
                     NCNGKQIREYGLGSEKVEEEVQAMFPGVRTLRWDWDTTRHKDAHEMILTHFSNHQADV
                     LVGTQMLSKGLDLPLVTLVGIVLADVGLNLPDPFASEKVFQTLTQVAGRAGRSSLGGK
                     VVLQTFMPEHYAIQFAAGHDVNGFYERELDYRKQLGYPPFAKLVRLEFRDADPLKAES
                     DSQKAADKLKLRIETENRRQTELIGPVPAFFSKVDGIHRWQIILRGPDPASLLRDLKL
                     SDCRVEVDPISLL"
     gene            complement(21223..21804)
                     /locus_tag="PL000_00125"
     CDS             complement(21223..21804)
                     /locus_tag="PL000_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87326.1"
                     /translation="MKNALLVLFLVSVLTACTPAITSAPTETILPMIVLSSTPIPTST
                     LIPATETPSPVPTDPIILMITPDSIQVERWREYQTALAKTIMSYELALCEWEVLGRSE
                     NEIYVWATCRILDNGSDSPAVIHLGVDGAIQKVEIPGTNWSSDIREMFPIEIQEIFLN
                     NLMNYQQLSDHLTWRIEHPEEPPLIVLSSTPAP"
     gene            complement(21995..24241)
                     /locus_tag="PL000_00130"
     CDS             complement(21995..24241)
                     /locus_tag="PL000_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HNC87327.1"
                     /translation="MKVWMKRIFSGLGILLLIGILYAAFAPLPQDEVLPPEKWGAGSS
                     SVEPAYSGLQRDFPATNETDANATTPEKAELGRLLFFDPILSKNDDMSCATCHNPDLG
                     FGDGMQQALGANGKTLPRNAMSLWNVAYNTNFFWDGRAATLEEQMHTPITNADEMAGS
                     PEEIESQLKAIPAYVSLFEQAFGSKDAVTYENVQNAIAAFERTLITNNSPFDRYAAGD
                     VNALTPAQRRGLATFRSAGTRCFECHAAPTFSDDSFSVTGVPDLPGQEHDAGRAEVES
                     ESLDGAFKAPSLRNIALSAPYMHNGAFATLEEVVEFYAKGGGRDDGVENVDIHVLGFD
                     LTKQEKSDLVAFMYALTDETNLPEFPESVPSGLPVVAHVENPARETISQMNAGGTESV
                     STSTHEAVTLRVEKGQSIQSVVDQALPGDTIEVPYGIYKEHVIIDISDIKFFGIPNEE
                     GQWPVIEGEGKGADGVIASGNNFEMAFFAVKNFTSNGVLVEGAKNVYLHDMYIENTGV
                     YGVYPVRCTDVLIERIEGTLMNDAAIYAGKSENVIIRDTLTYGNVIGVELENTVNGEV
                     YNNVAHDNTIGIFIDLLPQLPSKVSMYTKVHDNVVENNNGANFGKPGTAVSLIPPGTG
                     MLILAADHVEVYNNTIRGNKTGGMAVFNLTIGFSEEEIDVGPNPEHNYIHDNIYENNG
                     YDADKFVKDMLGQGFDIIWDGTGADNRFDEPNASSSFPPVLPSKNLPAPLYNLYWRAL
                     NFVVGLVG"
     gene            complement(24263..24634)
                     /locus_tag="PL000_00135"
     CDS             complement(24263..24634)
                     /locus_tag="PL000_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC87328.1"
                     /translation="MKKTILLLIALALSACSAMAKAEPTPTPDPLVLQGQQVFNAKCA
                     TCHALVPDTIIIGPSLNGIATRAETRVEGVNAEEYIQLSILRPGDYVVEGFNNVMITN
                     FAKELTNEDMNALVAFLLTLK"
     gene            24744..25640
                     /locus_tag="PL000_00140"
     CDS             24744..25640
                     /locus_tag="PL000_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87329.1"
                     /translation="MQSFSRGWAFLKQAWEMAFKDKDLLKPSIYALIVGMIVSVIGII
                     PIVGAAILFGDTQIGNIILFVLGGILVFVQFVVTYVFSGMTVHLIYGYLTEGDGDMGK
                     AWAIVRRDFFDILTLAAASTAVNLLRSLANRNRRGGVAASVARAATGLLETLWTEAAL
                     LILPAMIIDDLNLKDGMQRILKITKENLLLVGISTVGVRWVTGLIGFVFGAIGLVIAF
                     IIGGGAAFVSGGSTAISIIGIVIGALIFFTFVMLANLFSSYTNTAYHTCLYIWARQVE
                     TARAHGSAEQVRAPAPLAAALA"
     gene            25712..26221
                     /locus_tag="PL000_00145"
     CDS             25712..26221
                     /locus_tag="PL000_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNC87330.1"
                     /translation="MQPEPRREMVTWQEIDRLIDHLLPQFKREFTAMVMITRGGIIPG
                     GLLADAMDITHILTAAVDFPAQSKTEKSNLMVWPEFIQFPAEDKLRGRPTLIVDDVWG
                     SGRTITAVKNRVSAAGGFAETCVLHFNPYRNLFGNARPDYYAAVTDAYIVYPWEVDRG
                     ADKVLLGEL"
     gene            26229..26906
                     /locus_tag="PL000_00150"
     CDS             26229..26906
                     /locus_tag="PL000_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC87331.1"
                     /translation="MKTIEVSHENRIKTLKGNEYFDNLPEPMLKDISEHMHLREYQRG
                     DVLFWEGDPCDGLYIVEQGSAKIFRLSPQGRQYIVRILQEGDTFAEVPAFDGAANPVN
                     VEALEACRVWVIDGELLRRLVELHPHFAQKVLANFGRMLRGMVRMVSEMAFYQVTHRL
                     ARLIAEMPQERSAPHWTQEQLAARLGTVREVVARSMRELERSGAIKVEDRRIQIVDQN
                     IFSQWME"
     gene            complement(26971..27417)
                     /locus_tag="PL000_00155"
     CDS             complement(26971..27417)
                     /locus_tag="PL000_00155"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HNC87332.1"
                     /translation="MKKLILLLLALILTACSAGAASEFDKNLAKWEAAKITHYRYDVA
                     IGCFCAFRDDMPMTIEINNGEIVSITSVKGNIITPSDGLYDVVKSYAGMEALFEQLKG
                     AFEDADRVEVIYDTVNGYPTSIAIDQIEAAVDDELYISVENFEVLK"
     gene            complement(27518..28855)
                     /locus_tag="PL000_00160"
     CDS             complement(27518..28855)
                     /locus_tag="PL000_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNC87333.1"
                     /translation="MKNYTNLILAGLVIVLAAALVGVLTFVKNQPAQERATEPLVEIA
                     AMEPDSAKWGVNFPNQYDSFMKTQTNNIDTTYAGSSQFSWLERDPRQVILFAGYPFSK
                     DYNDDRGHANALEDVRATGRLNLDDSNPKHTPATCYSCKSTDNPGLWAEMGMEAYDKM
                     SFNEMTPNINNAIGCANCHEAETMRLIVTNPALEEAFERQGKDWTTFTRQEMRTVVCA
                     NCHVEYYFKGDGKYLTFPWDNGTKVDEMLAYYEEAGFKDWEYPDTGTPMLKAQHPEYE
                     FFTADSTHYKAGVSCADCHMPYTRDGAAKYSSHDVHSPLLNAEEACGQCHTDTEFVVN
                     RVNTIQEQVATTKITTEDAILDAVNAIKAAVAAGNADPALLDQARKLHREAQYMWDIV
                     SAENSTGFHNPEYALKVLAEATNRARQAQMLAAQSINDTSLLATGTYYATPAP"
     gene            complement(28867..29355)
                     /gene="nrfH"
                     /locus_tag="PL000_00165"
     CDS             complement(28867..29355)
                     /gene="nrfH"
                     /locus_tag="PL000_00165"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HNC87334.1"
                     /translation="MSKTPFFIAIAATVLAFGYFAFVTDAASYGGSAPETCANCHVMD
                     AQYENWYHGAHENFAKCTDCHLPHDNVVTYYAEKGRQGAKDVFAFVTGNIPVQIRASE
                     KTKGIIQANCVHCHEDTVEVVVMNAQPFDRYCWDCHRSVAHGDRGASAIPFQDSTLYP
                     IK"
     gene            complement(29657..30148)
                     /locus_tag="PL000_00170"
     CDS             complement(29657..30148)
                     /locus_tag="PL000_00170"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HNC87335.1"
                     /translation="MKRILMMLVLVGTLLLSACGSTMGDEHADGTDIEAHDFWVRSAL
                     KDGNSAAYMLLHNHTEADDELVGASSDAAASVEIHLSQMKSDGTMEMIQQESIPLPAE
                     GELELKPGSYHIMLIGLTKDLNVGDEITLTLHFKNHEDITLTIPVMEAQDMGGSGMDG
                     HMP"
     gene            complement(30194..30751)
                     /locus_tag="PL000_00175"
     CDS             complement(30194..30751)
                     /locus_tag="PL000_00175"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HNC87336.1"
                     /translation="MTSTIDREKKILEHLQKQGSASIQELVEVFNVSNMTIHRELNKL
                     AEAGLIQKKHGGVALVTQSPTAGINQCIMCNKTISERTVFIVRTTSGEQKRACCAHCG
                     LMVQSQTQDAAQSLTADYLHGHIISTTQAVYVLGSDLKVCCVPSVLSFGSQEDAEKFQ
                     KGFGGTLATMKDAIQFIHGMMHAHQ"
     gene            complement(30900..31796)
                     /locus_tag="PL000_00180"
     CDS             complement(30900..31796)
                     /locus_tag="PL000_00180"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC87337.1"
                     /translation="MNPYLAAAQAGKNDWWRYLISFFTILTIWFVIGSIPIILLLGYV
                     MMDGNPATNFTQSGFTGISVLLDFSATMLTFFPFIAATIFSIRVIHARPLRTLITSET
                     NVRWHRILTGAAAWFGVAALISITEALLYPGRYVFSLQPLLLLVYTLLALILIPIQTS
                     AEEFFFRGYLLQWIGLKLKNKWLLAFINGVLFFIPHMVNPEMAVNGILMGLNYFAFGF
                     FACLITLQDNGMELALGLHAANNLFGALFANYTVTALPSPALFTVQTLDPVYGLISTT
                     IGMIVFYWIVFRKQPQPAVILE"
     gene            complement(32123..33025)
                     /locus_tag="PL000_00185"
     CDS             complement(32123..33025)
                     /locus_tag="PL000_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNC87338.1"
                     /translation="MKQNSSASGKFMKFLQFPVTRFFLGFFLIGLVTSIAQIGTEVWP
                     GHVNNLAQIASVLIIVTIASLAYYGFVRVIEKRAVTELSPHQAVRELTGGAALGALLF
                     SATIGILWLFGFYKVSGTNAWTVLIPGLVLSISSGVIEEILIRGLLFRIMEESLGTWI
                     ALAISAVIFGFLHLANPNATLWGAIAIAIEAGIMLAAAYVFTRRLWLPIGIHFAWNFS
                     QGAIFGVAVSGNEVQGLLQSTLSGPTLLSGGAFGAEASIIAVMICLLAGIALTTLAMK
                     KGNFIQPFWAKHKPELKQENATPA"
     gene            complement(33030..33914)
                     /locus_tag="PL000_00190"
     CDS             complement(33030..33914)
                     /locus_tag="PL000_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HNC87339.1"
                     /translation="MFKTMEEYLDALKTEMKDADPALVQDAQADAREHLSTALEAARE
                     ANPAVSEADALGKIIEEYGTPEETASAYREVERRTSPALKQTTKPRSALGRFFGVYTD
                     PRAWGALLYMLIAFVTGVFYFSWAVTGLSVSISFLIFIFGFPIALLFLLSVRGLALLE
                     GRLVEALLGIRMPRRPLFSHQGMKWFDRLKALLTDKATWLMLVYMIVQFVLGTAYFII
                     IVMVLSISLSFIALPFIQEVLHQGAVYNNGVQYYFPSWSYPLLVLGGLLLWTTFMNII
                     RGVGQLHGRLAKWLLVSE"
     gene            complement(33919..34275)
                     /locus_tag="PL000_00195"
     CDS             complement(33919..34275)
                     /locus_tag="PL000_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC87340.1"
                     /translation="MTEISSHTRKFQKELNAGTSSLVLLSVLSRSKEPMYGYQIAKML
                     EESGPEIPMMKQGTLYPVLRSLEENGLLESTVEPSVSGPPRRYYKITNDGREALKEWA
                     EIWKITKKFVDANLKG"
     gene            complement(34425..35336)
                     /locus_tag="PL000_00200"
     CDS             complement(34425..35336)
                     /locus_tag="PL000_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC87341.1"
                     /translation="MKKFLKWVGIVLGILAGLVAIGLVIIYVQSESRLTHVYEIPAEQ
                     VVIPTDAASIENGKHIFQFRGCEACHGENLEGTVYLDDPAVGKVISSNLTSGLGGVGS
                     AMSDSDWVRAIRHGIRPNGTAYLFMPSTEFYYLSDDDLGDVIAYIKSVAPVDNDLPAS
                     EISFTGRIVMTLVKDITFIPAELIPHDSARPTAPKAGTTPEYGAYLTQSCHVCHGLNM
                     SGGVIPGFPSTWPPAPNITFGGGSALPTWTEEEFMNTIRTGKTPQGKELRAAYMPWNS
                     YKFMSDDELKAVWAYLQSLPRLEYGNR"
     gene            complement(35418..36329)
                     /locus_tag="PL000_00205"
     CDS             complement(35418..36329)
                     /locus_tag="PL000_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNC87342.1"
                     /translation="MTAQIIDGTAIAQKVRDEVKEKVAARIAAGKSQPGLATVLVGDR
                     VDSAAYVGMKQKACAELGMTSYHHPLPADVSQEDLEKLIKELNADPKVNGILVQLPLP
                     SHLDEERVLQLISIEKDVDGFSPINLGRLAQKGREPLFVPCTPYGCIYLLKEAGVTIK
                     GANAVVLGRSNIVGMPAALLLVKEDATVTVVHSRTKDIPGVLRQADIVIAAIGRAEMV
                     RGDWLKPGAAVIDVGINGIPMLNPDGTPVISQKTGKPRQKLVGDVNYEEAKEVAGFIT
                     PVPGGVGPMTIAMLMNNTLRAAEIQQP"
     gene            complement(36486..37139)
                     /locus_tag="PL000_00210"
     CDS             complement(36486..37139)
                     /locus_tag="PL000_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87343.1"
                     /translation="MNRLFACFSLVILFTSACTVAAPSGTATQDQAVPSGTVLFQDDF
                     ANLGSGWSRFSAAEGVMDYDSGSFRILVNALDTNFWSTPQKNFTDVRLEVDAGKLGGP
                     DENRIGLICRSSGADYYFFIITSDGYYGVGLFSGGQAVLLGQNEMLTSENINKGLAVN
                     HLRADCTGDQLTFYVNGFQIAAVQDATLKSGDVGMLVGTFAKPGVDVILDNFVALKP"
     gene            complement(37253..38323)
                     /gene="lgt"
                     /locus_tag="PL000_00215"
     CDS             complement(37253..38323)
                     /gene="lgt"
                     /locus_tag="PL000_00215"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC87344.1"
                     /translation="MDPVIFQFKLFGGEIVLRWYGVLVMLGAVVGAWLAEKEITRRGQ
                     NGEVIWDAMVWVLPAGIIGARLWYVLNSILGGSSYYTDNPGKILQVWEGGLHFFGGLL
                     FGALALYFFLKANGLDFWLFLDAIAPAVFIGQALARPANFINQELYGQPTKLPWGISI
                     DASHRLAQYADLSQFPVETTRFHPTFAYEMILNILLALLLLWVARQYEEKLKPGAIFA
                     GWLVLAGLARAFIEFFRPDQPHIGDSFVTFTMLVSLLMAVAGAVMLMVRYGRLQLAFA
                     ANWEEEYQLKKVEAAPAPKRKTSVASTAKDSDEETEIEMQDEDEEMEETPAPVKKRKT
                     AAATTSTSTTKKKSTTKSKTSK"
     gene            38568..39071
                     /locus_tag="PL000_00220"
     CDS             38568..39071
                     /locus_tag="PL000_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC87345.1"
                     /translation="MLRINRQTDYAVRVILALSKKEEGKRVSTSEVGREMLIPPALLQ
                     RIVAELASGGFILTQAGRDGGITLAHSPKQITLLQIVEHFEGELYLSDCVLKTGDCPF
                     ELRCPVHCQWVRLKNMLRNEMAQTTFQQLVDEGQQIEANLIKNASSAPIPDSAPIKIL
                     LPEEIPA"
     gene            39068..39742
                     /locus_tag="PL000_00225"
     CDS             39068..39742
                     /locus_tag="PL000_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNC87346.1"
                     /translation="MNKYFLGSFYGLFGLIVVSAFAFKVFQPIKVLPRMQLSPAFSLT
                     DQDSNRLTSEDLRGQFVLYTFEYTNCPEVCNSINDTMLEVQSRLGEVELDGISVAFVT
                     ISMDPERDTPDALNAYAQSIGADTNRWKFATTTNEAMLKTIIGSGFETYYEKKEDGSF
                     AFDPSFVLVDGWGIVRAEYRYATEVSNADRILRHLGVLAEEVRNSKGTTKLAYEAAHL
                     FLCYAP"
     gene            39756..40343
                     /locus_tag="PL000_00230"
     CDS             39756..40343
                     /locus_tag="PL000_00230"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNC87347.1"
                     /translation="MKTKILLGTFAIILGLVTGVYFFRPHTFHGTVIQSPETSYDFNL
                     TGGKGDVSLSDFRGKLVLIYFGYTFCPDVCPATLGQVNQAMKKLGSKAEDIQLIMISL
                     DPERDTPAKLEEYVAHFNPTFLGITGTQEQVDTVASLYGIFYEKKEGSAASGYLIDHT
                     ATLMVIDREGYLKLVFPFGVTADEIADDLKYMLKQ"
     gene            40456..40593
                     /locus_tag="PL000_00235"
     CDS             40456..40593
                     /locus_tag="PL000_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 2A"
                     /protein_id="HNC87348.1"
                     /translation="MAKKDKEDDEFRPTGTIAVLAIFVVTLIAIWATIYMILVGRGGT
                     L"
     gene            40590..41090
                     /locus_tag="PL000_00240"
     CDS             40590..41090
                     /locus_tag="PL000_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNC87349.1"
                     /translation="MSTNQIHIDSYERNWMIVSAILLVLFAAAVSFAAFGMGIQVPAP
                     EQRVDPRTVATDPNSPWSNPGLREIEPGKYDAYILARAIPSWEYLPKELTVPAGSSVS
                     FYVTSSDVQHGFKIQDTNANFMVIPGQVSKLTITFDKPGTYNFICTEYCGSGHGVMYG
                     AIIVTE"
     gene            41108..42757
                     /locus_tag="PL000_00245"
     CDS             41108..42757
                     /locus_tag="PL000_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="b(o/a)3-type cytochrome-c oxidase subunit 1"
                     /protein_id="HNC87350.1"
                     /translation="MKQYQLSAGEKKFIGSHLLVAILALAVGSLFGPLQAFEHAGWDF
                     YVYLKPFIKSYYQGLTVHGVLNALVWTTFFIMGFLTFNVIFGLKRPLKNMALNKVGFW
                     MMVVGVVAAAIPILANQATVLFTFYPPLKANWAFYVGLTLVVVGSWVEGWGLFMTFAA
                     WRKENPDERSPFIAFASVVTALLWQICTLGVAVEILTMLLPWSLGLIEGVDPQLARTY
                     FWFTGHPLVYFWLLPAYISWYAMLPKQAGNGKMFSDSLARLAFWIFLVLSTPIGIHHQ
                     YVDPGVSPVWKFLHSMFTFGVFFPSMMTAFTVVASLEHAGRKQGGLGMFGWIGKLNWS
                     DPSVATQLLAGILFFFGGIGGLTNASYNVNLVLHNTAFIPGHFHLTVATAVTLSFMGI
                     SYWLLPYLTGRKLWNPKWAVAQAWTWFVGMIIFSNSMHVLGLLGAPRRVPLGLAPYVP
                     AEWNSRLFQVGVGGAILFVSGAIYLTVMLMTALNKEKVNEEVIVPIAESMQEVSRTPV
                     WLDRWAPWIYGALGLIVIMYGPVLFEMIKNIALTSPGFRVW"
     gene            42910..45252
                     /locus_tag="PL000_00250"
     CDS             42910..45252
                     /locus_tag="PL000_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNC87351.1"
                     /translation="MATSPTKKQLIDVLTERNPSVDWKARLNSPSRRLLNFFESISLR
                     LEAPVNWLINDPRFNPLYHTGTITIFLLVVILLTGIYLTMFYPFGFTFSYQAISKIEA
                     NLIGRIIRAMHRYASDAAVIFALLHGWRTFFQDRFRGPRWLAWVTGVGMAVVVWAIGI
                     TGYWLIWDERAQLLNQSLFSILGSFKNGQTFIVDFIVGDAAGTGWVFMMIVIVLHLGL
                     SALTAFFLWLHLKRMSRAKWLPPRYWMAISVFVLLLSAALFPLGMLPPLNATQLPAEA
                     PVDLFYLFYLSTFLRGPQALFWSVLLFIVGLVTALPWILPRAKELEPVKVDLANCDGC
                     TLCERDCPYLAIKMVPRTDGARPKFQAEIDPNLCVSCGVCIGSCPDNALTFGDIPLNP
                     LWKTTLERIAQDDKSKVKVVFTCERHALHGAGKHFEDPNTYIVPLTCIAMANPNLAAQ
                     ALEAGASDVQFIGCPPEDCANREGNVWLNDRVSGERLPKLKQNFIPLVHTAWAAPADF
                     GKAIKTQIKSEPNTFKFTPNASHLRFIIPLLGVMAVVTAFQIWLSDWPTKFFNEDSAV
                     LAIQMTHHSGYPVQDVTPLPPLEPDLEQPIRITLEVDHELVFDETYTATDNHINQGAR
                     IFEHIHLPVGRHHITVKMYDRADASFEQILFDDTVNLEPQQALTINFRDVHIPDPKAG
                     EQLYYETASGVNAGCRICHSLEKGETIIGPSFYGIANRAGERVPGLTAEEYLHQSIVD
                     PNAYVVSGYPSGQMIQNFGTILTEEEIQDLIAFLLTLDGQ"
     gene            45315..45740
                     /locus_tag="PL000_00255"
     CDS             45315..45740
                     /locus_tag="PL000_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87352.1"
                     /translation="MRRFSLLILLIALGLSACGRNKPGPLGADASADNIPDIVGSYAL
                     NATDPTGEEYGGTLTITQGGQANEYKFQWLISGGIQEGTGTLEGNQLIFTWNSLAGTD
                     QTVSGTGQYTITVDGQLYGTRTIDGLDTPGKETAYPNPK"
     gene            46001..47440
                     /locus_tag="PL000_00260"
     CDS             46001..47440
                     /locus_tag="PL000_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC87353.1"
                     /translation="MAKVVVIGAGFAGHTAALYLGSRLGKKHEITVISNRDVFGYVPS
                     WVWVGVGHMKPEQTIFKLKPVYDKMNVKFVHAAAKEIHPDAGDQYVLGEGVASGQEVR
                     LDYDYLVIAPGPLLNFAGTPGLGPENGFTHSICSLPHAIKTRDAYLALCDRMKKGEKV
                     KLVMGTGHPGATCQGAAFEYIVNVHKDLVKKGLRDKADILWLSNEPAIGDFGVNGVQI
                     KRNGHIMRSEEFLTSVFNDHGIRYQVQTGVTKVEEGKIHWQNYDGEEGTTDFDFSMMI
                     PQFKGQPFKYIDKNGEDITSKITNPAGFVLVDGKYGLPYPELAQTPEAWPSQYQNPIY
                     KNVFAAGIAFAPPGPISKPFTNKNNLLIAPAPPRTGMVSGVIGRVVALNIVDLIEKGE
                     MTHREPMTEMVAACIASMGDSLWDGNAVSIVMQPVVPDRKKYQNEFGRDLFTSHLEMG
                     VSGAWMKFMLHHTFLWKFKARPGWTIIPE"
     gene            47899..49179
                     /gene="rho"
                     /locus_tag="PL000_00265"
     CDS             47899..49179
                     /gene="rho"
                     /locus_tag="PL000_00265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNC87354.1"
                     /translation="MKILELENEPLSKLRGMAKTLNIPNANRLKKETLAMMIREAEAQ
                     KEGIELRGGILEIMSEGIGFLRATNYRISDQDVYVSQAQLRKYDLRAGDLVIGQVRPP
                     RESERHFGLLKVESINGLEPEPGNSRRVVFENLTPIFPDKRFDLEIGHDQLAPRLINL
                     IAPIGRGQRGLIVSPPKAGKTTILKQIANSISTKYPDVHLIIALIGERPEEVTDMDRS
                     VDAEVVASTFDEPVTAHVRTAELALERAKRLVEIGRHVVILMDSITRLARAYNLVVNP
                     SGRTLSGGMDPSALYPPKRFFGAARNVEDGGSLTIIATCLVDTGSRLDDVVYEEFKGT
                     GNMELILSRKLQERRIFPAVDIERSSTRREDLLLGPDLLQRVWLMRRMFIQMITPPPG
                     GAGMDLSVATEAIITRMERAKNNIEFLENLTKDA"
     gene            49213..50388
                     /locus_tag="PL000_00270"
     CDS             49213..50388
                     /locus_tag="PL000_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC87355.1"
                     /translation="MKTLFEKINAFFTSIGGRFKRKPKEVSAVNEPPTTPRGQGAKPR
                     VDRFTIISWAVTLLIVVSLLGSTLFYKSTLANAAPVVVQPTPEEAPVAGQPQVGVPAA
                     GSGGGGVLSILRRLQLKTNIPERPRYASTTYRVSRGDAMLSIADDFKLKSESILFAND
                     QLEDNPHNLKPGMELTIPPVDGVYYEWQEGDTFESVAEKFSANADDIVNFPGNDVDLT
                     DPKIEPGTTVMVPGGTRDLRNWAADLQTMGRGAGTGTGGGNATNACGGGPVATGFGWP
                     ADDHSLSGNPYGPSHLGIDISAPEGSNVYAAGAGVVTQAQGGWNYGYGNVVQIDHGNG
                     YVTVYAHLSSYVVSVCQFVGQGAVIGYSGNTGNSFGAHLHFEIRIGGQNINPYDIVQ"
     gene            50439..51224
                     /locus_tag="PL000_00275"
     CDS             50439..51224
                     /locus_tag="PL000_00275"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNC87356.1"
                     /translation="MNENSLKKALSKLKLGGLRYFDSIGSTNDEALAWAATEGGAADL
                     SLVVADEQTQGRGRLNRKWFTPKGSALAFSLILRSSETLRPNLSRTVGLAALSIAESC
                     SKNGLTPSIKWPNDILINGKKLAGILVETVWSGDELDLLVIGVGINVFKSSVPPADLL
                     QFPATSLEDELGTPPNREEVLSSILEALIHWRARMGTDEFIKAWENQLAFRGQQVQTF
                     AGTDVPVVGELLGLEPDGSLRLLDKHRKSIVVRFGDVSLRPDA"
     gene            51252..51485
                     /locus_tag="PL000_00280"
     CDS             51252..51485
                     /locus_tag="PL000_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87357.1"
                     /translation="MNEFDNLREDAASSYDEAPAPKYLEVKGPAPTFTAKPRSRRILG
                     MTGQQRFILSVMFMLAVCVIGFMALFITGKIGF"
     gene            complement(51564..>52985)
                     /locus_tag="PL000_00285"
     CDS             complement(51564..>52985)
                     /locus_tag="PL000_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNC87358.1"
                     /translation="VITRRSKFDLAKAQARLHILDGYLIALNNIDAIIKVIREAEDVD
                     TAKANLVKRFKLSEVQAQAILDMQLRRLAALERWKIEEEHKQVRETIAYLEDLLAHPK
                     KILALIKSDLNELAETYGDDRRTRIAAEAQENIHEEDLVPDESVLISLTERGYIKRVA
                     ANAFKSQSRGGRGVMGHTTKDEDEVMMLIPARSLNTMLFFSDKGKVYSEKVYQIPDSD
                     RATKGIPLVNVLSLDPNEKITAAMAVGDFSSHNFCMLMTSRGKIKRVSMEEFASVRPS
                     GLIAMSLEEGDQLGWARLTSGKDEIIIVTENGQALRFSEAKVRAMGRQAAGVQGIKLK
                     KDDAVTSMDVVEKDGALLVVTTRGFGKQTPLSDYAAKGRATSGNYTIDPKALKEIGKI
                     AAARVVQKADDLTIMTSNGVTLRLKVKDVKQSGRATKGVHLIKPQDGDNVASVARISA
                     EDLKKAGAQVLEAEKVEEQPKLV"
     assembly_gap    52986..53085
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<53086..54253)
                     /locus_tag="PL000_00290"
     CDS             complement(<53086..54253)
                     /locus_tag="PL000_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC87359.1"
                     /translation="MAEENLNTGNIESIDIDAQMRSAYIDYAMSVIVARALPDARDGL
                     KPVHRRILYAMHELGIRANTAYKKSARIVGEVLGKYHPHGDSAVYESMARMAQDFSMR
                     YLLVDGQGNFGSVDGDSPAAMRYTEARLQKMAEEMLADLEKDTVDFGPNFDDSLEEPL
                     VLPARLPNLLLNGSSGIAVGMATNIPPQNLRELVGAINYLIDHHDTIDDVTVNDLMKF
                     VQGPDFPTGGMIVGADGILSAYGTGRGRIVMRGIAHIEETKAGRYQINITEIPYQVNK
                     STLIERIAELVRDDKIDQISDLRDESDQRGMSIVIELKRGAQPKKVLNQLYKYTALQS
                     TFGVQMLALVADESGRGEPRTLPLKRMLQIFIEHRQTVITRRSKFDLAKAQARLH"
     gene            complement(54440..54523)
                     /locus_tag="PL000_00295"
     tRNA            complement(54440..54523)
                     /locus_tag="PL000_00295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54487..54489),aa:Leu,seq:cag)
     gene            complement(54904..55125)
                     /locus_tag="PL000_00300"
     CDS             complement(54904..55125)
                     /locus_tag="PL000_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC87360.1"
                     /translation="MEQENKTASGLLLPETAKEKPQTGQIVAVGDDESIKLKPKDKVL
                     FAKYSGTEFKYEGVDYLLLEASDVLARLN"
     gene            55211..55501
                     /locus_tag="PL000_00305"
     CDS             55211..55501
                     /locus_tag="PL000_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87361.1"
                     /translation="MEVNALHTKIDKTIQVNSGLIVLEVPNGFPRSESNLYLVGLRGD
                     ILWKAEKPDANTLYSKVKLNEDGETFSAYTLNGHACDLELKTGKLITFTSIQ"
     gene            55562..55840
                     /locus_tag="PL000_00310"
     CDS             55562..55840
                     /locus_tag="PL000_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87362.1"
                     /translation="MTQPPLGFVLVFLLFSLLFLSNTYKLWFKTDTYYQDLYNSLTNE
                     KTPHPFKNYFLRRLQNRKRWELEQKLFSLVGLAAVIGADVLVVTAYLG"
     gene            55923..56690
                     /locus_tag="PL000_00315"
     CDS             55923..56690
                     /locus_tag="PL000_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC87363.1"
                     /translation="MKIIACIKQVPDSEAKVTASGGQVSWGDAPLVINPFDEYAVEGA
                     LQQKEATGATVTALCIGPESAKEALKHALAMGADEAILVSDAALNDLDTVGAAKVLAA
                     AIQKIGADMVVFGRQTLDNGAGLTPAQTARVLGWPMLGLAGQIKVDGGSVQVERVLEE
                     GRQSVKASMPAVLSVVQSIGEPRYPSFMGIRKASKANIPVWSLNDIGASAPAPMITRT
                     ELMTPPVRDTAVEMIAGETPAEIAEKLADKIIAEKVL"
     gene            56687..57700
                     /locus_tag="PL000_00320"
     CDS             56687..57700
                     /locus_tag="PL000_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNC87364.1"
                     /translation="MKTFVYIDHFKGDVQPASWEALGLAKSFGTATALVFGTGADDVA
                     KAALEYGADEVLLADDSLLTDYRAETYASTLSALASSHSPNLILLPTTARTREMAAMA
                     AIDLNTGVLTDVSGLEANGDSFVATRPIYEGKLIEKTVCSGKPVMATIRGRAFPKPER
                     AAGKSGTITKVSAAGEAKSTVEGYSAAESAVNLGDAGIIVSGGRGVSNNPSLTPPAGM
                     DEKQAEVWRAQQGFALITDLATLLGGAVGASRAAVDGGYITYAHQVGQTGKVVSPDLY
                     IACGVSGAIQHIVGMRNAKVIVAINKDPDAPIFKVARYGVVGDLFAVVPALTEAMKKK
                     LGK"
     gene            complement(57802..58131)
                     /locus_tag="PL000_00325"
     CDS             complement(57802..58131)
                     /locus_tag="PL000_00325"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNC87365.1"
                     /translation="MSAASIWLLFLAIGLGTFTLRFLFIYLFGKIEMPDWLRRALRFV
                     PAAALAALVFPALTHPTGHLDISLQNFRLLAGLGGAVVAWKTRNVLLTLIIGMILLWV
                     LEAAFGV"
     gene            complement(58128..58823)
                     /locus_tag="PL000_00330"
     CDS             complement(58128..58823)
                     /locus_tag="PL000_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNC87366.1"
                     /translation="MNKDFIAGARGSLPILIGVIPFAMITGVAATSVGLTLMETIGMS
                     VLVFAGASQMVVFQLMTAGSPWIIMVLTAWVINLRFTMYSATLAPYLQKLSTWRKALL
                     AYILSDQAFGISLSHFVTTNEEINHRWYYFGSALIVWVAWQISAILGALLGSLVPASW
                     GFDFAFPLSFMALMFAALRDRPTVIAALAGGFIAVLAKGLPYNTGLVLATFLGIMAGY
                     WAESIQARKGTIQ"
     gene            58995..59495
                     /locus_tag="PL000_00335"
     CDS             58995..59495
                     /locus_tag="PL000_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87367.1"
                     /translation="MRYGSMYRMLFVLLCFSMLSLACNAVTEAMSTPPPVVPTVFTGG
                     QESQPSATQETESDPEPQATEAGEIPFGIIGTAPEDIPILPDASEMMASPNSVMYTTQ
                     LSKDEASQFYVTEMKKLGWSLTPNASINGDVANILSFTMDGKTASIIIGTATGKVKVQ
                     VVISEN"
     gene            59925..60950
                     /locus_tag="PL000_00340"
     CDS             59925..60950
                     /locus_tag="PL000_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87368.1"
                     /translation="MENKTSLLLIDDDPALLVGLEAVLRYEGYRVFTARHGRTGIDIA
                     RESTPDLIICDLMMPPPNGMEVLTILGQDPRTASIPFIFLTARTSSTDKVHGLLSGAD
                     DYVIKPFVKDELLARVKTILRRKQKAPVPSSATDKDEIQHLRNEITEILRNSEVNWEK
                     FVDSLVHMLGLRNNETEDHAWRVMNLTEKTAIELGIHGVELIHIRWGAILHDIGKVGI
                     PDNILLKPGELTKEERNVMMMHPQIASQILEPLGLPSTTLDIPLYHHERWDGKGYPDH
                     LAGDNIPLSARIFSVVDVWDALTNDRPYRKAWSCEKAGDYILGEAGKSFDPHVVDVFL
                     KKVISYS"
     gene            60969..62087
                     /locus_tag="PL000_00345"
     CDS             60969..62087
                     /locus_tag="PL000_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNC87369.1"
                     /translation="MSDSRKTILVIDDEFHLLVGVKALLEHEGYNTVSTSDGMAGVQM
                     ARKVQPNLIICDVMMPIMDGFKVRETLSEDPVTKDIPFLFLSARASQTDKLRGLNNGA
                     DDYITKPFDHRELLARISAVFNRYEKGQGDMRRKMDEEILRFEREISHNISHELRTPM
                     TQILLALDMALRGKYENIDDLKLFVETALSQSHKLNGLIDDLIFLNNYDRGMVNTLRQ
                     EVDLKADFMEPIKFLQDLYAEKNIKTQVKIDPTVTIHAPRREFKRAIGHLVDNAFKFS
                     NPMTTILIDLEENEVGGFVLTVTDYGHGFAQQYSEKVFNRFFQIDQGDCRAYNGLGVG
                     LTVARIFARSLGGDVTIFPSTRGCRVQMMVPPGSTEIP"
     gene            62103..63095
                     /locus_tag="PL000_00350"
     CDS             62103..63095
                     /locus_tag="PL000_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC87370.1"
                     /translation="MHTNKTFRWAVILLLLASIGLSGCASQKKPIRIAISAWAGVEPA
                     ELAAQLGYYEKYGVDVELVRFSAYSDSLEALRDENVDGGMHTLDDSLRNMAAGRDVRV
                     VLLTDYSFGGDGLVARAGIENLSDLRGTKIGVEIGTVGHLSALKILEKAGVAVSDVTF
                     ISIPAWEIQQAMIDGQIDAGVTWEPYLTSTAEMIGGKVLITSREYPETIVTTMTFNAK
                     LIDARPDDVQKIVAAYFDAVEYIKQNPQEAYTIMGKAEGISAEEFSTHVMGIRYLDLS
                     SNAELFGVNSDGRVYAAAQEISVFLADNNVITTMPDVQQMLEPTFVLTLFKDGQ"
     gene            63117..65846
                     /locus_tag="PL000_00355"
     CDS             63117..65846
                     /locus_tag="PL000_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87371.1"
                     /translation="MNSLKTKLTIYQGLVGFAGILLIVSITWLVSRQRILSNAIAEAE
                     NLSDQIAYSMVVIAAQGGDATFNYQRLIEKTSTLNNVSSIKVLDEKGVILADNDHSQV
                     GQVFNSPLLEDSLKNQRKEQQIEGRSLIVVRPLRGETYTANLNDVTGFLWIELDFTPA
                     YARAQEDMSLILAVSLGGFLLTFYWYYRVTQNGILDRLSVLSSGLIKVENGDLTQRIS
                     IRKLFGSTDEINELASQFNRMTASLHQKLTFEELTSHLSAKFMNVPFSEMDTAVHDTL
                     RRLGELFKVDRTYIFEFTDDGLAMNNTFEWCANEIIPQIDNLQGVPREIVPWWMQLLE
                     NGEIIIIPRVSEMPKEAASERQILSEQGIQSVLVTPMTSDHGLFGFIGFDSVAHERNW
                     TKEEVNLLSMLSGVVANTLIRQRSQREVVEERDFALQVMETVAHGLIVADLDGSLEYT
                     NPAYAKMLKYTAQEITGRKIQEFMHIEDIPQDETQNSYTAQMRLIAKDGTVVNTLVSR
                     SPRIKDGHIKGSIGAVVDLTEQLLAEEKLRQSEARNRAFLNAIPDLIFRMDEAGRFID
                     FKAGDDSRFYIPPEEIIGASVADILPAEVSNMTLLAIKNSLQTSKPQSFEYKLQTPSG
                     LFTYEARVAASSPGEVIAVIHDITERARLEQMKTDFINRASHELRTPLTTALLMVNLL
                     ERSYIKPEEREEYWQILTQELNRERIILEDVLTVGRLEAGKYRIADGLVSIMPVLKSA
                     IDSMQLQASMRNVEFQVDAPETPPDLRGTEEVFARIFSNIVSNAVKFSRPETSIQVRV
                     IPNADGMSVEVQDHGIGIPPEDLPHITSRFFRGTNATEMEIPGSGIGLYIIKALTEEL
                     GGKLGIQSKLNDGTTISLWFPIPSIKKDENPASHSADDWSGEKDSSAPKVASS"
     gene            complement(65841..66653)
                     /locus_tag="PL000_00360"
     CDS             complement(65841..66653)
                     /locus_tag="PL000_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNC87372.1"
                     /translation="MFTEMQNHQKLIPTDVRVLAQRKEEGLEKQLLVFQTPFGYRRMA
                     ELFLPEGEGPFPTILFIHWYEPEARDSNRSQFVSEAEELARKGVICLTVETLWSDPDF
                     FLKRTQADDPGNSIEEVVNLRRFIDFLSQQPKMDPKRFACVGHDFGGMYGVLAGSADR
                     RPTHYVIMAATPRFPDWYLYLPKLEGDAREAFINQMAEIDPIMHVSKLAPAPILFQFG
                     NDDFHVPLERADEFFAAAQGPKEMKVYTSGHGLNADATHERKEWLQKQLLGS"
     assembly_gap    66833..66932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            67132..67809
                     /locus_tag="PL000_00365"
     CDS             67132..67809
                     /locus_tag="PL000_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87373.1"
                     /translation="MSKLLSRFSYSNLKEQLMKIWPVVLVGIFVSLGGAGITVLVAAL
                     TDNPIWKLAKDPAEVMQFPSYIGMLSNWGVLLWIASAVICLFGVAVLKQYEVPKDTLR
                     FITGSGLLSLILGIDDLYRLHDKIFPKMFHLPEKFFYILYIFIFLLYLAMFAARIMEY
                     EFLLFGAAFFLLVVSRRIVVIIPFFNQFMTTGDMLKYFGIVFWLAFFYRTVLHEITSL
                     INKNKTA"
     gene            68151..69422
                     /gene="serS"
                     /locus_tag="PL000_00370"
     CDS             68151..69422
                     /gene="serS"
                     /locus_tag="PL000_00370"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC87374.1"
                     /translation="MLDINLIRETPDVVRKALKDRQMDASPVDAILQLDEKRRTLLGQ
                     VETLKAERNTVSKEIGQMKDAAARQSKIEAMRVVGDQIAELDKQVAEVETQLSDLTSA
                     LPNIPDARTPYGKDDSENVILRTVGEPRKFDFKPLPHWDLGPALGILDFERGTKLTGS
                     RFYVLQKAGARLQRALIAWMLDLHTRQGYQEEYLPFMVKTAVVFGAGQLPKFADNLYK
                     DHEEDYYFVPTAEVPLTGLHMDEIVEEATLPKLYTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYTYCKPEESEAQHEKMLKDAEETCALLGVPYRVKRLCTGDLGFGSTMTYD
                     LELWAPGCDEWLEVSSISNVTDFQARRANIKYRPSDGGKAKFVHTLNGSGLGLPRTLI
                     AVMENYQQADGSVVVPEVLRPWMGGIDVIKP"
     gene            69488..70009
                     /locus_tag="PL000_00375"
     CDS             69488..70009
                     /locus_tag="PL000_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HNC87375.1"
                     /translation="MDPEALKLFAEVFLEVTTFVILLFGLFGLLIPVFPGLTVMWIAT
                     LVYAIVRLSVGNMTWVGWLLFVVITLLMVGGNIADNIIIAKHVRDKNVPWSSIIWAFA
                     AGIVVSLFLTPLAGIIASPIGLYLAEWYRLKEKQPAFDNTKAWMTGWGWSLAARFGIG
                     VVMIAFWGLWAWI"
     gene            complement(70089..70430)
                     /locus_tag="PL000_00380"
     CDS             complement(70089..70430)
                     /locus_tag="PL000_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87376.1"
                     /translation="MAKNKNFEPSDAEDYLSQVKWKSDHAYRRTPWYLMPKWKYKPVS
                     PYQNESLDPAFQQGFALILFLIPVGFSVYLLFSGYFWQAFAILAFTSFVGAILFLAIR
                     DAQKKLKDKDD"
     gene            complement(70446..70916)
                     /locus_tag="PL000_00385"
     CDS             complement(70446..70916)
                     /locus_tag="PL000_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87377.1"
                     /translation="MGDYNRSTVEINFAHISPDIMQAVHQHIETHNFGDILNGVTHCI
                     VSTSEKIKKGLFSGPGPKVLVETVILTNRWLILADQMDTKPVHVRSMQLRDLVIEDYE
                     KSSFQSLIPDTGLSITGRFTDVIEQGMIFLPLGKDNAGDTFKEAIIRAVQEAKK"
     gene            complement(70976..71977)
                     /locus_tag="PL000_00390"
     CDS             complement(70976..71977)
                     /locus_tag="PL000_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNC87378.1"
                     /translation="MKQLLKTLTESFGPSGYEDDIRKIVLKEVKPLADEVRVDALGNV
                     IARKKPASGAKNPKKIMFAAHMDEIGVIVSHIDKNGFVRFTNIGGTFGRYTLGARVRF
                     LNGVSGVVGYDRLEQMDHWLPIDKMYIDVGATSKKDCPVKVGDVAAFERPFVEMGNRF
                     VAKSLDDRSGVVAVIEAMRAVKSTPNDLYFVFTTQEEVGTRGAGTAAYGIDPDIGIAV
                     DVTPTGDTPSSLKMVMELGKGPCVKFRDMGMLSDPRVVDWMLQTAEKAKIPHQREVLL
                     VGSTDAREIQVSRAGVMTGAVSIPVRYVHSSSEMVDYDDMKNTVKLLAALASKSVNF"
     gene            complement(71981..72700)
                     /locus_tag="PL000_00395"
     CDS             complement(71981..72700)
                     /locus_tag="PL000_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNC87379.1"
                     /translation="MNGLIALLGSGEYLSVMDDVDRHLLANCGVTDRKPRVVCLPTAA
                     GREGDASVNRWSNMGVEHFTRLGADVQAVPVIDAESANDLNHASVVEDADIVYFSGGD
                     PSYLHRTMKDSLVWQAAQKAWARGAVYAGCSAGAMILGREIPDFRAMGMRSIPAFGSI
                     AAAFVMPHFDAIPLFGKPLVSALRGRLKAGEMMIGIDENTAIVGRQNEEWIVLGHAKA
                     HVFTKNESRSYSAGEKFSLGN"
     gene            complement(72704..73759)
                     /locus_tag="PL000_00400"
     CDS             complement(72704..73759)
                     /locus_tag="PL000_00400"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC87380.1"
                     /translation="MLSIGTTQIKLLEKLCNAMSVSGDEGEVRRIVLEEVKPFADEVK
                     VDALGSVLVRKHAAASSGKKPLRVLLDAHMDEVGFMIVSDEGDGFYQFEAVGGIDSRH
                     LVGKQVIVGKDHSLGVIGAKPIHLASSGEMEHSLSIDSLKIDLGLEGKAKVGDRATFA
                     TKFKKVGPSIMSKSIDDRIGVAILIELLKNAPRNIELCLAFSVQEEIGLRGAKVAAHY
                     FEPDLAIAVDSTPARDLPDYDGNENTKYNTQLGLGPAIYVANSSTIDDPRLVKFLQDT
                     AVKNKIPHQLRQPGGGGTDAGAIQLTRAGVPVVSVSVPHRYTHSPISVSRVDDWKNTL
                     NLLHTALKAMSPDLLKR"
     gene            complement(73765..74853)
                     /locus_tag="PL000_00405"
     CDS             complement(73765..74853)
                     /locus_tag="PL000_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNC87381.1"
                     /translation="MSDILPFLKSIISVAGLSGHESPVADLIEKQWTPLVDEISRSKL
                     GSLHGLKKGTPSKGISKPEKSPRPSVLIATHMDAIGLMVTRIVDGFLYITNIGGIDHR
                     VLPGTPVTVHATGSGEELYGVIVMPPGNLLPEGKGEGAVNLKYLMVDTGLLPADVSKR
                     VRVGDRVSFNTEPIEMSGGLVSGHNLDNRASVAALTVCLEELQSKSHAWDVWALASVQ
                     EEVTYAGAYTSAFQLRPTIAVALDMTFGKGAGANGYQTFPLGKGLTLGIGPSVHPFLH
                     KRMKEVAERVEIPVKDEAMPEMSSTDADAMQLTAEGIPTVVVSIPQRYMHTPVEVVAL
                     KDIQRAGRLLAEFITSLEEDFLTKIVWD"
     gene            complement(74902..75450)
                     /locus_tag="PL000_00410"
     CDS             complement(74902..75450)
                     /locus_tag="PL000_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87382.1"
                     /translation="MMLDYNRPDMSETEAPRTRNRMRALAVILATIPCYCIGFIFLVL
                     APDVKPNATPTTTILTVTGTPSTPTQSVTPPIITNTITQIVIVTNTLTQTATPTYTPF
                     QANTVTLTFTLTPSITPTFTLTNTLTPSITPSVTLTSTPTNTLTITPSPTITLTPSLT
                     PTITLTPTPTNTPSPSPTAFGG"
     gene            75454..78042
                     /locus_tag="PL000_00415"
     CDS             75454..78042
                     /locus_tag="PL000_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HNC87383.1"
                     /translation="MIPLRLRISGFLSYRDPVELDFDSFDLACISGHNGAGKSSLLDA
                     FTWVLFGEARGKSADIINLNSDVKAAEVALTFKYEGNVFRVQRTLPRNKSTLLEFQVL
                     DEGEQKADLESGTWKPLTEKTTRDTQARIEQTLRLDYDTFINASFFLQGKADEFTQKK
                     AGDRKAVLSSILGLEVWDEYKDRAADKRKIVEREVDEIDGRIAEIDSELAEEDARKAR
                     LAELETTLGQLSTSRAAQEAALANIRKNADLLNEQRKLTEALSTALERARIALLGLEK
                     RLTEKESERTAYTDLVHRAKEIESSYKAWQKTRKELESLDQIAMEFREHEKERTPLLE
                     QIATEKARLEEERRSLLVEQEEIENQSASMGDLTAEVEKARTALQDAEIRIAERTELD
                     SVRNDARERQAFLKAENDALKENMNSLKERMDSLDAADGATCPLCGQELTEKHRKATV
                     KQLEAEGKQKGDKFRANKSEMDQLEKTLADCTARLAKLTGAENDRLKFSNTISQLTER
                     IETLGVVAKEWEKSGKKRLAEVTKVLEKESFAADARKGLAKLDKELAKLGYDVATHEE
                     TRQQELELREVEEEYRKLESARELSKRIAKEIDELEEEIKKKQAEVSESEEAHRAAAQ
                     ALAESESHHPNLDDVERDLFALREDENRIRDQVGAARQKVEVLATLRGRKADYAVQRE
                     ELNQQIVRYKTLERAFGKDGVPALLIEQALPQIEQKANDLLDRLSDGQMSIRFVTQAE
                     YKDKKRDDLRETLDIQISDSAGQRDYEMYSGGEAFRVNFAIRLALSEILSQRKGARLQ
                     TLVIDEGFGSQDAQGRQRLIEAINLIKQDFAKILVITHLDELKDAFPNRIEIEKTERG
                     SAVKVV"
     gene            78086..79252
                     /locus_tag="PL000_00420"
     CDS             78086..79252
                     /locus_tag="PL000_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC87384.1"
                     /translation="MPYFITSTILFVAALVIIYWIHNQYHLDIVVTSANPPADAPLFS
                     ICIPARNEENNISRCVESALAQDYPNIEVIVLDDRSTDATLTQLKEIAARDSRLHPIS
                     GSDLPAGWAGKPHALFQASAVAHGDWLCFVDADTFLEPGAISACYAKALETKADLFTT
                     MNQQVLGSFWEKVVMPLVMTALSVGFSPRKVNDPTRRDAVANGQFIMIRRAVYDAIGG
                     HEKVKDQIVEDKAISEQVKWNGHRLVVADGSRFIRTRMYTSLETMWEGWTKNIYLGLR
                     DHPAMLMLGAFGATLALIAALFLPIWPMLGLGWYLQGGGALAIWVILEALVVWGALLL
                     ARARVARKMFISAWYAWTTPLGAGVFAAMMLASAWKVISGQGVTWRGRKYHPAK"
     gene            79410..79931
                     /locus_tag="PL000_00425"
     CDS             79410..79931
                     /locus_tag="PL000_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87385.1"
                     /translation="MNEPSASSENDQVQVSNLSQVDVEMVQAELVRMHQSSAQEIQAE
                     DVELGMSAAAEVKAANATVRDSVVGLLQATDASMQDNLVGVARAETMSLNGGAGVVVA
                     GSVEFGNAYAGVVAGREVRGEKIESVILLSRNVHGNVTTVMDTRGALIAGLVGGLFAG
                     IMLLLGRMLFGRK"
     gene            80028..80684
                     /locus_tag="PL000_00430"
     CDS             80028..80684
                     /locus_tag="PL000_00430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNC87386.1"
                     /translation="MEKVVLKASKRDVVGKQVKALRRNGQLPAVIYGRHVEPIAISLD
                     AHSTGLAFTKLTSSTLVTIDVDGTEYAALVREKQRNYIKGNLTHVDFLAVDLTEKITT
                     KVHLTFTGVAPAVKDYSAVLVHRMEQLEVECLPTDLPERILVDISSIKAIGNSIRVRD
                     IQLPANVAVLEDEDEIVMIATLAKEETTEAAAAAGTEAAAPALSVERGKKDEEGAEKK
                     "
     gene            complement(80777..81709)
                     /locus_tag="PL000_00435"
     CDS             complement(80777..81709)
                     /locus_tag="PL000_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87387.1"
                     /translation="MKKHPLLLASLLTLITACTQLTGPAPTQTPAHSLPTVVALTGQA
                     AFATASALVPTSAPPTETLVPTETPVPTTPLPSATPTVAAGFNDFAEIHFLSPGPMSS
                     LVSPINLQVILIAGESEIVQVDLLGEDGRVLERVLERVQRNLAGNYRSFELSFEIRAV
                     SEKGYIRISSKDDHGRIQALNTMPVLLYSVGVNQVNPLGNMIYERIIYEGLEDGDEIF
                     GGTINLKGRFWPFNEQPAFLELMLPDGEVISSRVLDFNGIETQEFETTLPYKVTEPTL
                     ARLSIRQDNPDISISDPDLKKYVYIYTMEVMLYP"
     gene            complement(81786..83282)
                     /locus_tag="PL000_00440"
     CDS             complement(81786..83282)
                     /locus_tag="PL000_00440"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HNC87388.1"
                     /translation="MSEKLNQLKEILGEVNDLYHAASVLEWDQNVSMPPGGSEARGQQ
                     LSTLGKIAHEKSTSDEVGKLLDELKKEYTDPNTDDGALIRVASRNYDKSTRVPASFVA
                     RQALTTSKAFDAWVEAKGKSDFALFQPHLERVLELVREYVSFFPPADHPYDTLLDDYE
                     PGMKTAEVQGIFEKLRPQQVKLLKAIKSSKQVKDDFLQKKYNEKKVWAFSEAIITKFG
                     YDWNRGRQDKAPHPFQTTFSVNDARITNRYEPNNPMATLFSAMHEAGHAFYELGSNPA
                     YERTPLAGGTSLAVHESQSRLWENLVGRSLPFWEHFYPDLKKAFPSQLDGVSLKAFYK
                     GINKVESSFIRVNADEATYNLHVMLRLELEVGMVDGSFAVKDLPEIWNTKMQEYLGIV
                     PPNNALGVLQDVHWSNGLMGYFSTYALGNLVSAQLWEKINKDIKDLDDQIRKGKFDTL
                     LHWLQEKIHTFGHKYDPQDIVQKVTGSRIDSAAYVRYLNTKYSDIYGL"
     gene            complement(83465..84181)
                     /locus_tag="PL000_00445"
     CDS             complement(83465..84181)
                     /locus_tag="PL000_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC87389.1"
                     /translation="MTKTFQIKRSILTAALIVVLLQACMQVRVDAPLALPTPQDIETV
                     AVESPAATATASTTPTTAPATATETLPSATPQPEVVIHAVKGNLFIRRGPDMAFNPIG
                     VLYKDTSTPVLARDVLSNWVQIVIPDSDQKGWVSLQTQYSQVDGDLRTLPELAPTDWP
                     LPAYLRNCTHHQMYILPAEVILLSSLDHPDNMVWLNPGTYTVYDMDVAGEPEVTTVEV
                     REGVTVEILDDGAGEHRKCP"
     gene            complement(84190..85449)
                     /locus_tag="PL000_00450"
     CDS             complement(84190..85449)
                     /locus_tag="PL000_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87390.1"
                     /translation="MSFSQALLEAIESLNGNKMRSGLTVLGIVIGVAAVIAMLAVGNG
                     AQASITGSISSIGTNLLFVFKGSPDGPPGPGGGGRGSGNNDRPLTLSDAEAIADPLAA
                     PSVEIVAPAIQGNGTITFAGENTTTTISGVTPNYAPVRNLEIAEGEFINEEHILGRMS
                     VVVLGPETANAIFGHADGIVGETIRIEGQPFRVIGVLVAKGGGAFGSEDNSAYIPFTT
                     AQARLIKRTSLDEIDVLFVQATTAESVPQAADEISNILRQRHRTPIGDDDFTVFTQQD
                     FLQTFETITGVLTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKKD
                     ILLQFLTESSLLSLIGGVIGVIFGWLIAFAVGQVATATGNNFVPIVGTDAILLSTSFS
                     AIIGLFFGIYPASRAANLEPVEALRYE"
     gene            complement(85454..86149)
                     /locus_tag="PL000_00455"
     CDS             complement(85454..86149)
                     /locus_tag="PL000_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87391.1"
                     /translation="MDLVIEARDLRKTYQMGEIIVEALRGVSFTIERGEVVSIMGPSG
                     SGKSTMMNTLGCLDRPTSGDYILDGESVAEMNDDQLASVRNRKVGFVFQSFNLLSRLT
                     ALGNVELPLRYAGITEGRRERAQAALEAVGLKDRMLHRPYELSGGQQQRVAVARSLVN
                     NPAIIMADEPTGNLDSKVGKEIMSLLLNLNKDRGTTLIIVTHDPTIAEQTQRVIRLRD
                     GELDPASEIKRKG"
     gene            complement(86151..87407)
                     /locus_tag="PL000_00460"
     CDS             complement(86151..87407)
                     /locus_tag="PL000_00460"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC87392.1"
                     /translation="MKQFFSKITRRTWVIIGVLAVVVLAIVFFSTRSSGEPLYQTTTV
                     ERGNLVATVGATGSVRARQSAVLIWQTTGIVKDIYMEVGNPVKRDEILASLDKNSLNQ
                     SVILAESDLAAAEKALADLLNSDTARAQALRTVDAAEEAYKKAYDYRLELNGLITITE
                     TYLVRGVPKVRTYKGYADAETIADADEKLALAEAQLNDARRNLDRLQNGVDPAELAAA
                     EGRVAAAQSTMNMAHIIAPFDGVITQSEPSVGDQVTAGTVAFRIDDVSHMLVDLQVSE
                     VDINNVAIGQPAVLTFDAILGKEYHGEVVEVGQAGDTVQGVVSFTVTVELTDADELVK
                     PGMTAAVNVVIEEQKDMLLIPNRAVRLVDGERVVYLLVDGQPVQVKIKLGSSSDTMSV
                     LAEGDIKEGDLIILNPPSNNGGPFGG"
     gene            87719..89161
                     /locus_tag="PL000_00465"
     CDS             87719..89161
                     /locus_tag="PL000_00465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC87393.1"
                     /translation="MQAVPSEFLAIDRKERANRPFFDLDLRPAEVRTCDFDDVVIEFD
                     PERAMVEAARCIHCPDPAPCMVACPTANDIPSAMWLIEQGRFVEAAGIYHKTSSLPEV
                     CGRVCPHEALCQGSCVLNKHHQPVLCGELEAFTVDYERRTQGYVIPLGIPSGKKVAVI
                     GAGPAGLGCAEVLVQKGHDVTIFESKPAPGGLLVYGIPNFKLSKEVWQEKWGEFERAG
                     VKFVPNTYIGKDKTIDGLFEEGFEAVFIGVGSEVDAKMEDTPGTELPGVYEATDFLIR
                     GNVERDQLPEHMRAPLEVGKRVVVIGGGDTASDCLRTAIRLGSKDVTCLYRRTEKEMP
                     GGKKDRKMAREEGAKYRFLTQPVKFIAGSDGKLAAVECIEMKLGEPDAKGRRKPVPVE
                     GSNFSVAADTAILALGYWPDPIIGKTTPELEVHDWGLITVKDKARGNTSRPGVFSGGD
                     CVTGPDLVVTAMVGGRKAALAIDEYLKNKK"
     gene            89254..90528
                     /locus_tag="PL000_00470"
     CDS             89254..90528
                     /locus_tag="PL000_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87394.1"
                     /translation="MFQRIIRGLEITLILFIIAAITGHGNPSLTDKIEKVRAFTRQIE
                     FNYMAWMGNAALLKLRSASVGVPYTLNYESRKQIVTEYLRVTQSILENEYQLQQIYAD
                     AAITDKELASEELRAELDEQYARQAKLAPLAEATLQEQVTIILSEIGLAAVGQPVPNV
                     WYHSTPLPYALIVSPRDRIEQIANISINTEITLDEQAALESRVDAGLNTSSLVVPIGG
                     VGVYPTMVMRSTNLPWVLNTISHEWIHNYLTLRPLGFLYANTPELRTMNETTASIAGN
                     EIGALVIERFYPELKPASRLSPGLISAPFDHPEPGDFLRPPFDFRAEMHETRITVDAM
                     LAEGQIEEAEAYMEARRLLFLQNGYLLRKINQAYFAFYGAYADSPGGAAGEDPVGPAV
                     RALREQSESLADFVNTISWMTNFEQLQEAIKK"
     gene            90625..91869
                     /locus_tag="PL000_00475"
     CDS             90625..91869
                     /locus_tag="PL000_00475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC87395.1"
                     /translation="MKRIVFFLIISIVSMIGLSACAAEAETAPVTVAVQPTSLPDVAT
                     PISESSCSAIVVAPTPTPGVDSYFPPATADDFSYGSADAPVTIIEYCDFQSPGCGAMA
                     LVFAEFLRSQENVRFVFRPVPLLDVMDKSDKATMAAIAAGEQGQFWNMYDLLYLRQQE
                     WINMEPRAFDDWVVREAVILGLDGEKLTAAMNAPQTEASMRSMYEAAKKLRIPAVPLV
                     LINNSPQSANILNYRNLSETVGLIALGQKQFSECPPFDIDPVKQYIATVKTEKGDIVI
                     QLFPDKAPLAVNSFVFLARQGWFSGVTFHRVLPGFVAQAGDPSGTGRGNPGYFFNNEF
                     SDLKFDKPGVVGMANSGLNMNGSQFFITFGPASHLDGGYTIFGQVISGLDVAEQLTPR
                     DPDQAVIPAPGDKIISVEIEEK"
     gene            91869..93122
                     /locus_tag="PL000_00480"
     CDS             91869..93122
                     /locus_tag="PL000_00480"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNC87396.1"
                     /translation="MQTKNTHWPSILILIVLGTSVLFVFLFIAATGISSIIDLFSESG
                     DPAGEMISTFAFGFELLVLLVCMWFVFQKAQSREQADLQFKFPFANWQILAVLGLIGL
                     SVMLGAAVIYTEVIALAWVVLPFLTLLVIVPPIWIFFGLGTKGIDFGSRWRVFSILGL
                     GMTLAPVTMIVLELIILFVIIIAGTVFLALSQPDLLQEVAAVGRILQNETDQDVILNL
                     VAPYLSSPAVIATLIGYIGFIVPLIEELLKPLGVWLFARKFTSPAQGFAMGMLSGGAF
                     ALVESLNASGNGSAEWVVVVSVRAGTSLLHMVASGLVGWGIVSAFQERRYLRLPAAYF
                     CAVAIHGLWNSCAIGMGISSVGESLGRPEWLAAYAPSMLGGLVVLGVGMFVVLLAANR
                     KLSSVAVISPTPPAETRAETEEKVK"
     gene            93127..93957
                     /locus_tag="PL000_00485"
     CDS             93127..93957
                     /locus_tag="PL000_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HNC87397.1"
                     /translation="MIDSTITHDNDLIQLVGLTHKHFIFSLKAGAELHTHRGMIKHDD
                     LIGKPWGSQVFSHTGAPFFLLQPSIADILNDLPRATQILYPKDIGYILIQMGVAPGQR
                     VMEAGTGSGSMTIALATAVGANGRVISYEQKQDTQTLAKKNLERVGLASRVEFKLRNI
                     QEGFDETGVDAFFLDVQNPYDYIPQVRAALKPGGFFCTLIPTFNQVEKTLIALRKNQF
                     AFVEVCELMLRHYKADSARIRPTDRMVAHTGFLIFGRRIEPNTDPRGKELEQEIGMEE
                     "
     gene            94009..94554
                     /locus_tag="PL000_00490"
     CDS             94009..94554
                     /locus_tag="PL000_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87398.1"
                     /translation="MMFRRRGQQRMFRGGRREGPPVLQQANQLMLDGEYEEASSLFAD
                     LGQRAEERFPHRAPFLFIEAGRAAILGGQTKTGVAHLRRGLTLFASQGRVHRMRMVGE
                     RAIDELQARGLKAEAEEIAALLGSNSLPDASGEKEIPAPTKRPVLPTHCPSCGAAVRP
                     DEVEWLDEVTAECAYCGSPVR"
     gene            94563..95168
                     /locus_tag="PL000_00495"
     CDS             94563..95168
                     /locus_tag="PL000_00495"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNC87399.1"
                     /translation="MNLTEEYITQKLQAAMRSSTDADGYAEMAVHPDTRLKCAAVLVP
                     LTQVNGEWHLLFTRRTEIVQSHKGQVSFPGGACDEGETTPEQTALREAEEEIGLRPAD
                     VRVIGRLSNLITISSFRVSPIVGVIPWPYTFKVAGVEVARVFTIPLLWLSDRNNYWEF
                     FVRDSERSLIAYHPYDGELLWGATARMTVTFLKTIETLARK"
     gene            complement(95170..95532)
                     /locus_tag="PL000_00500"
     CDS             complement(95170..95532)
                     /locus_tag="PL000_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87400.1"
                     /translation="MTHKCQAIVVHCMDYRLQSHINQWLESNFEKGTYDRAVMAGGVY
                     DVYDVIRQVDIAARLHGISKVILINHEDCGMYGPGGTEERHEEDLDKAEDKIRRLFPH
                     LEVDTFYLNLDGTFEKNS"
     gene            complement(95545..95922)
                     /locus_tag="PL000_00505"
     CDS             complement(95545..95922)
                     /locus_tag="PL000_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87401.1"
                     /translation="MPAKKPIIHCDALVVHCMDYRLQKFIQPWITVRFGYDNFDIISL
                     AGSVHDYEMVLKYVQLAVLIHSIETVCLINHEDCRAYGRDGTYKRHKHDLKDAQAKIK
                     KLFPHLHVETFYLHLDGTFEPIS"
     gene            complement(95964..97376)
                     /gene="hydA"
                     /locus_tag="PL000_00510"
     CDS             complement(95964..97376)
                     /gene="hydA"
                     /locus_tag="PL000_00510"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HNC87402.1"
                     /translation="MNTLIKNGTLVTASDTFAADILIEGETIAQIGRDLHHPNAEVID
                     ATDRLIMPGGIDPHVHLDLPMFGTVSSDDHYTGHKAAAFGGTTTVMDFVVLENEGFDH
                     SVDLWMKKAEKAAIDYSFHMNLTNFNDKIAKEIPSLMKMGIQTLKVFTAYNGRLRIDD
                     GSIFKALRIAKENGMLMMAHCENGDVIEALIPEALNAGHTTPEWHAYTRPAWGAVEAT
                     LRMAAMAEQADSPVYIVHMNAGGEVDMLKYARENGVKVMGETCPQYLFFTIDHLYRPD
                     GAKWICSPPMRTEQDNARLWEGLSGGVLQTVGTDHCPFYFDGTSPIVYEGSEIAIPGK
                     ELGKDDFTKIPNGLPGIQDRMPIMWTYGVRAGYITPNQFVAYNCTNPAKIFGLYPRKG
                     ALIPGADADIVIWDPEKQVKYGVAHSHQRTDYNLYEGWELAGYPEKVFLRGKLIVDGE
                     EWKGKRGDGQFLKRGAGEIL"
     gene            complement(97544..98131)
                     /locus_tag="PL000_00515"
     CDS             complement(97544..98131)
                     /locus_tag="PL000_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87403.1"
                     /translation="MLKATDLVLVALIPTPRDMEIARLLGWYRIPLRTAPKVVAVDYL
                     AFYQPAAFGERSGQIECIAQVKGHELTTRGELLKDERNHPRAKEEYYKIQLGGLEKLK
                     EPIKTDKWKRLTFLYSTGDYILNAKTLNDLVVEGGERQLLWKNLRERAENEQLYKTDL
                     PEADIPPEVLLALLGIKELQEPYEAGAPNAQKENE"
     gene            complement(98209..99570)
                     /locus_tag="PL000_00520"
     CDS             complement(98209..99570)
                     /locus_tag="PL000_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC87404.1"
                     /translation="MNLSFLSLTDQRDLIKSREINASELADACYRQIERLNPTLNAFI
                     TVIPPEQLSAQEETQSGLLQEIPIAVKDLYNTKGIRTTAGTRFFADHIPQEDAYVVQK
                     IKKAGAQIIGKTNTHEIALGVTNNNPHYGACRNPWDITRTPGGSSGGSAVAVSTGMAM
                     AALGTDTGGSIRIPASLCGVVGLKPTFGRVSLRGILPLSWNLDHAGPITRKVEDAALM
                     LQVMGGYDELDPASMKTLPGDYYSHLRDGIKDRKFALATGSFIEESDPEVLEAVRKTA
                     QVLEEQGAVITETNVDFLKEAAVANALMTQADGAAFHRERLKEHPDWFGADVRQRLEI
                     GAAFTSSEYILARRTQAEVKRQLELLFEEYDGLLLPTTPITAPVLEGENALERARQLT
                     RFTAPFNLTGLPALTIPCGFTSEGLPIGLQIVSRAWNEAGVLRTGYAFQQATEWHTRM
                     PSI"
     gene            99675..100262
                     /locus_tag="PL000_00525"
     CDS             99675..100262
                     /locus_tag="PL000_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87405.1"
                     /translation="MKKTKTILLAMTVLLALWVSACGGSAAPATQAAVPPVVESAPTQ
                     APVVESAPVQEQPLATVQSFAPACQAAASCAAPAVTDTVASDRYCVEKIPYQNILVDD
                     GVTFEVLDPANLICSDNGTMVDGKHVVECHGTEAWSSQVKFSNTACSAAGLQTGTGQC
                     QEGLGYDAANNCCAPLSAGDAGSITITVNMGECPN"
     gene            complement(100349..102349)
                     /locus_tag="PL000_00530"
     CDS             complement(100349..102349)
                     /locus_tag="PL000_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNC87406.1"
                     /translation="MATKKSAKAKAAPKARKIVKKAVVKKAAPKAKAAFTPTKLKLVR
                     PVPSDIDIAQAAKLKPILQVAEELGVRPNELELFGPYKAKIKLEILERLKNKPNGKYI
                     DVTAITPTPLGEGKTTTTVGLSQALGAHLGKKVITAIRQPSMGPTFGIKGGAAGGGYS
                     QIIPMEDFNLHLTGDIHAITASHNLVAAALDVRVMHEKEQDDEKLFNALCPPNKKGER
                     KFSPTMLRRLAKLGIKKTNPNDLTPEERSRFARLDIDEKTITWRRVVDINDRMLREIE
                     VGRGKDEAGHAHMSGYDITVASEIMAVLALTTGLEDMRDRFGRMVVATNKRGEAVTVE
                     DLGVAGAVTVLMKDAIKPNLMQTLEGTPATVHAGPFANIAHGQSSIIADMIALKLVGK
                     DGFVVTESGFGADIGMEKFFNIKCRYSGLIPQVVVMVATVRALKMHGGGPKVVAGKPL
                     APEYTDENLELLKAGLPNLERHIQNALKFGVNVVVAVNSFATDTKAEVEMIREAALKM
                     GAMDAVVSTHWADGGAGAKALAEAVVKAASKPSNFQFLYPLENPIKSKIEDIASKIYR
                     ADGVDYTPEAEEQIERYTRLGFDKLPICMAKTHLSFSTDATKKGAPTGFRITVREIRA
                     SVGAGFLYPILGDMRTMPGLPTRPSFYDVDLDLETGKVLGLF"
     gene            complement(102450..103247)
                     /locus_tag="PL000_00535"
     CDS             complement(102450..103247)
                     /locus_tag="PL000_00535"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HNC87407.1"
                     /translation="MKTEIVLLKLGGSLITDKDIPYTPRLDKLKELALEIKTVLDSTP
                     NLLLILGHGSGSFGHVAAKKHGTRNGVQTAEQWQGFAEVRFQAAELNRYVMEALYNAG
                     VPAISFPPSSSLVSKNRKVIAHNSLTIRKSFDFHILPVVYGDVAFDEALGGTILSTED
                     VFTFLVEQFSPTRILLAGIEAGVWEDFPARTRLVKQIQLSDYEKMRAGIGGSVSTDVT
                     GGMKAKVEEMLDLIQKNPNLTVQIFSAEENGLLTRALRGENVGTLLK"
     gene            103308..104114
                     /locus_tag="PL000_00540"
     CDS             103308..104114
                     /locus_tag="PL000_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87408.1"
                     /translation="MDIHGGIIAAAILVAVAALFVVRASVRVMQNARKMSFYSLRRMH
                     NANALRLFLFALVLFGCSFWLPFFGEPIVYDFFPPSPTASLTPTITLTPTITLTPTIT
                     PIPSETPTPAVSDTPTLTFTPFLPVAIEALFSGPVTPNPDALFTPLQFSTEFDGLNPV
                     DPKTVFELPIQKMYGGFDYNNTLPGVQWTALWYRDGQLVCYETTPWNGGTGGIGGYTE
                     CDDPIGGWQAGTYEVQIFMGYDWKVVGQFIVHSELATPTPTSTPTMTPTP"
     gene            104217..104711
                     /locus_tag="PL000_00545"
     CDS             104217..104711
                     /locus_tag="PL000_00545"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC87409.1"
                     /translation="MTDKAFQDYYPEYFAHCYGCGSVNELGHQIKSFWDSEESVCYFQ
                     PKPYHTAIPGYVYGGLLASLIDCHGTGTAAAAGYRAENRAMDTEPPLRYLTASLHVDY
                     LKPTPMGPVLEVRANVKEVKGRKVVVEEWIVVNGVVTVRGEVVAVQVPEKLVLELLEG
                     KDGG"
     gene            complement(104852..105247)
                     /locus_tag="PL000_00550"
     CDS             complement(104852..105247)
                     /locus_tag="PL000_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC87410.1"
                     /translation="MMKAEFTLTATFNASADKIYQAFLSTQGHTLMTGSPAKVDGKVN
                     GEFTAWDGYIWGTFLELEENKKIVQAWHTAEFPEDAVDSHVEILLEEANGKTKLTLIH
                     THIPKGQAEGYQQGWEDFYFKPMREFFGG"
     gene            complement(105251..106177)
                     /locus_tag="PL000_00555"
     CDS             complement(105251..106177)
                     /locus_tag="PL000_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNC87411.1"
                     /translation="MKKNNLTLSLEVSRALNHGAPLVALESTVITHGLPHPQNLELAR
                     DMEKQIREAGATPATVALLDGEIRIGLSDLELVRLSEADSTLKVSHRDFATAIVKKAD
                     GGTTVAGTMFVANMAGIKVFATGGIGGVHKESPFDISTDLRSLAEIPTIVVCAGAKAI
                     LDLPATLEYLETMGVPVIGYRTDEFPAFYSRESGLGVSARLDTPKEIADFAKAHWNLG
                     MKSGILVTNPIPEAEAISKSEMEPMIAQASAEAIAQGIHGQKLTPFLLGRISELTKGK
                     SLKANLALLLNNARLAAEIAKEMSGKKREWAI"
     gene            complement(106177..107778)
                     /locus_tag="PL000_00560"
     CDS             complement(106177..107778)
                     /locus_tag="PL000_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87412.1"
                     /translation="MNKARRDELNTLLFSLTDEGVDFRNRWRSIAQIVVWLTLSGISM
                     LSAVFFTQATWQTAVVIGMSLVKYAPLMAVVYSLARKMASLYLDDIFELHDEELASQF
                     LEEVTFGYGLDKITINEGRVVEKDETSPIILIGGPGAIQVNLDSVALLEKVNGEPEVI
                     YPRKDAWKLGRFERIREIGKFDEVGKREYAIINLRDQFISGLTVKSRTKDGIPIEAQD
                     IKVIFSILRKPKPETPEAPDSSYLFDEEAVKALVYNQTIITPVPSTPSAVSFPWDTSA
                     IPLITSELENLITSHTLSEILVTIGQKEIDNTSNNEQTITQMRVELTGQQTALGSKKA
                     ASAPSFKSRSRITAQFFEKPFQDKAAAVGIYIEWIDIGTWQLPSSLILDKHKEAWNLA
                     RENAKKAASVERLRKRFETAEIVSLIENVVIRNYERPVSGSYRSSYSGSKEKEADKEW
                     DAFIAANPDLKGQLNPRESRPKDSTATATALEILKAFRKELLAGKELIEKENRPFEEK
                     QADLLKIEKALFDISHLTHHWIRKS"
     gene            complement(107775..109175)
                     /locus_tag="PL000_00565"
     CDS             complement(107775..109175)
                     /locus_tag="PL000_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87413.1"
                     /translation="MRKIFSGIGAFLQTPVGIILTTNVIWHIVVLVAKIPFNYFASAV
                     ILGLDIFAAFYLRDKLIYFFSQFVLPIQNPKHRREIAARVSNFESGRRGPALFVKNGR
                     VILHEGEAGKRGAGVIVLDTASALVLRTDTEIKDTVGPGIKFTSGNEYIAGSVDLRSQ
                     WQFIGPLASDQPFLNPASNPSKASNERRQQTSGMTRDGFEVSPTISIKFSIKRPVEKK
                     PTESGVTSQYGFDAEAVRNAITREVIQLGTTENTKVRMEWNKLPAHLVVNIWREYIRK
                     FKLSDLFTSSGVSGLQTIEDMINKRVKQSNVMALDDTGSPTGEWLESLEFKQLESRGL
                     EIQEVSIHNVLFEQEIENQVISQWSTEWMNLAKKEEGQLKEKEALIETASRDEGSKSF
                     ARIASRQFSGKPTMPQQNPFKTLQLLIQPLKEFILFESSANTDVEKELRKLDDIWKWL
                     LDNNPDGPRNQQGGQP"
     gene            109325..109888
                     /locus_tag="PL000_00570"
     CDS             109325..109888
                     /locus_tag="PL000_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87414.1"
                     /translation="MDTKRQNLKGMILVGGAVLVILIAVFVFRNFNTQGASAKAPMSA
                     TVTLSIDQGSFAASDDVLVHVTITNPNDTPIRILKWFTPINGLERSLFTITRNGETVP
                     YIGRMLKRAEPTEADYITLEAGGSITADVNLAEYYALSATDDYEVMYDVTSLQLYIEK
                     EIGELTNGRLSSTPLKMFITGRAAPAH"
     gene            109930..111576
                     /locus_tag="PL000_00575"
     CDS             109930..111576
                     /locus_tag="PL000_00575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HNC87415.1"
                     /translation="MKIGNARKLFYVAMILMLALAAFGSWDVSAARAQGSLGGATVRL
                     SVDRASFGTGDRVLVKVNISNPTNSQIKILKWLTPAMGVEAPIFSITRDGKAVAYLGK
                     LAKRMAPTDADYITLPPGGSISSEVDLAEYYDLSASGNYSVQYNIASANLYRWDQSQS
                     TDLLVSASVDLFVEGRANRVIQRVDPQAVVGTNTFNGCSSSQQTDLVNARNAASTYAA
                     DDVAYFAANKQGERYVTWFGVLDSARYNLVKSHFTNVQNAVDTASPMHFDCTCTDAGV
                     YAYVYPDSPYEIYLCGAFWSAPTTGTDSKAGTLIHEITHFTVVAGTDDYAYGQTAAKA
                     LAISNPAQAVMNADSHEYFSENNPPLEVSSGTFTDVPSTYWAYNFIERLYSAGITGGC
                     SSSPMMYCPESSVTRAQMAVFILRGEHGSAYVPPAATGTVFGDVSTSTFAAAWIEQFA
                     SEGITSGCGGGNYCPDSNVTRAQMAIFLLKGKYGSAYVPPVATGIFSDVPVGSFAADW
                     IEQLAAEGITSGCGGGNYCPSANVTRAEMAVFLVRAFNLP"
     gene            111802..112467
                     /locus_tag="PL000_00580"
     CDS             111802..112467
                     /locus_tag="PL000_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87416.1"
                     /translation="MSKAFFRVILKAKGLLVFLCFIFLTGCAGGTGAPANKTEFQPLS
                     SDSTSFYYYVSENRVPLTLSTEWIAVQFANEDPSAQASALKGSIVDANGQAEQLTRYG
                     VTLLPVQAGTTFETLVAGINALRSDSTFFQVNPVFQSGDTGMIVTDEFIVTFPAGMSM
                     DEINAVNASYGVEVGDPILGQENTFVLRATTATKMDTLSLANLYQESGVAMFAAPNFV
                     RVR"
     gene            112537..114816
                     /locus_tag="PL000_00585"
     CDS             112537..114816
                     /locus_tag="PL000_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC87417.1"
                     /translation="MKNSKTVRLFSKLLTVIALAAMVFSTFVSGNVSAAQAKGAQPSI
                     SSQAADGSFYYYADGKRIELNPSLSWVAVRFANDDLGAQSAAVDNSSALLGSLDQARR
                     ITPSSLALLPLKAGVTAATIRDAVKALRADTAGFALVNPVFQTSDAEMAISDEFVVTF
                     PAGMGMDEINAINAANGVELVDAILGQDNTFVLRVTGGADLLEMANKYQESGIAIHSA
                     PNFIRIVQKPAPAPSSPKPDFAPMAGTNDTYYPYQWHLNNIQQYGAGTTLDADIDAPE
                     AWDLTTGSSSIIIAVLDEGVDLSHEDYSSKLVPGYDATGGGSSGGPWGNDAHGTNVAG
                     LAASGSNNSLGVAGVCQACKIMPVRIAYSDANGNWVTTDATIANAITWAYQNGADILN
                     NSWGGGSEATVINTAFSNAKTLGRGGKGSVIVISAGNSNDPTVSYPAYLSTAIAVAAS
                     NLCDQRKAPINNLCNGYEYWWGTNYGSALDISSPGVWLFSTDITGAAGYASGNYFDQM
                     NGTSGAAPIVSGVAGLVLSVNPNLTSDEVQSILQSTADDVNGGGWDAEMGYGRVNAYN
                     AVVAAGAGSTTFSDVPATYWAYDFIERLYNAGITSGCSTSPMMYCPTASVTRAQMAIF
                     ILRGIHGSAYVPPAATGTVFSDVSTSTFGAAWIEQFAAEGITSGCGGGKFCPDDVVTR
                     AQMAIFLLKGEHGSSFVPPVATGIFPDVPVGSFAADWIEQLYNEGITSGCGGGNYCPN
                     SFVLRDQMAVFLVRAFNLP"
     gene            114946..116901
                     /locus_tag="PL000_00590"
     CDS             114946..116901
                     /locus_tag="PL000_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC87418.1"
                     /translation="MLQKTLVVFARKPMYLLMILGIIFSTFGTAQAQEFNGSIVPAGS
                     TQIQVDDEVFASLGQKGEARVIINLRDPVSIAAPSNERTSAIARIQGSVLGRLSSQDF
                     RVLRQYVNVPALAGHLTSSGLDILMSDPNVTSIDMDQMGGGHLSESVTALGADIVHSN
                     GYTGAGITVAVLDTGIDTDHPDLSDDLVAQHCFTDSQCKPGSTAEGTSAEDENGHGTN
                     VSGVISSGGVVSSVGFAPDASIVAVRVLDANSSGWVSDWLAGLDWIRTNQGTLGVDII
                     NMSLGTFALYSGHCDADNSSVANVVAQLNSLGIVIFASTGNQGSSTQMAMPACLSGVV
                     AVGATFDSDLGREPDSGTYQDIFGGNWPACANDTTNLQTITCFTNSNSVMDIVAPGAR
                     ITAPGMGGGTSTYSGTSQASPTAAGIAALMLQASPGLSPATIEATLKSTGTSVTDAKN
                     GLSFPLINALAAVNAVNTTFTDVPSTYWAYDFIERLYAAGITSGCSTSPMMYCPLSPV
                     TRAQMAIFILRGEHGSAYTPPAATGTVFSDVTTSTFGAAWIEQFASEGITSGCGNGMY
                     CPDANVTRAQMAIFLLRGKYGSAYVPPVATGVFTDVPVGSFAADWIEQLAAEGITSGC
                     GGGNYCPNAEVTRDQMAVFLVRTFNLP"
     gene            117060..118820
                     /locus_tag="PL000_00595"
     CDS             117060..118820
                     /locus_tag="PL000_00595"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC87419.1"
                     /translation="MNRKMLCVLIVLFSFGAVHVVSARPALALIDSPPSLGFAEIASG
                     LSSPVSITNAGDSSGRLFITLQGGQVVIYDGSQILPTPFLDVSSLIRNGSEQGLLSVA
                     FHPNYENNGYFYVYYTRLSDAALVIARYEVSADPNIADAGSASILLTIPHAEASNHNG
                     GQLQFGPDGYLYIGTGDGGTGGAYSQDGLSLLGKMLRINVNGAAPYSIPADNPFVSDP
                     SVADEIWALGLRNPWRFSFDSLTGDLIIADVGQNAWEEVNVQPASSNGGQNYGWPCYE
                     GMHDNGDTSTCTIGTITSPVVEYEHGASDTNGCSISGGYRYRGSEFQSLKGVYLYGDF
                     CTGRIWGAEPSNGSWVAVELADTDYGVTTFGEDENGELYVADFFGGVIYKIQATTFTD
                     VPLDHPQYAYIEALYDAGYTAGCSSSPLMYCPDTILDRAQSAVFMLRGQMGTGYTPPP
                     APWDTFTDDWTGFEWAQPWAEGMYQEGLTGGCQTSPLMYCPATQLPRVEASVFGLRMK
                     YGVNYIPPAATGTLFADFPATDPSYWAIDWAEQAYLDGLLPACGTDTVSGKPMFCPNE
                     LVDRGWGAFLIVNAKNLPLP"
     gene            119116..121278
                     /locus_tag="PL000_00600"
     CDS             119116..121278
                     /locus_tag="PL000_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC87420.1"
                     /translation="MKNSSHFFRFALVVIMLVAIFANTPVASASNSAQTQKAGAQTGF
                     VSDLYNTGDPVLTDIWVDAVNGNDANDGSSPASAFRTLTAAWNSIPTTNPLPQGRRIN
                     LQPGTYTESMIPNYWENRYGTFTAPIFIRGNGSSRGEVVLQGTISMYNVNYIYFENLS
                     MIFNGDVFHCELCNHILLRNTVLNGGMFAHETIKANQSQHLYVENSDISGSYENAIDY
                     VAVQYGHVVNNQIHDADDWCAYFKGGSAYLRFEANVVYNCGTGGFTAGQGTGFQYMVS
                     PWLQYETYDIKVVNNVIHDTDGAGLGVNGGYNILMAYNTLYRVGSVSHVIEVVFGGRT
                     CDGQAGDPGREMCQQNLNLGGWGTTAVDNGSNSVNIPDRNVFIYNNIVYNPAGFQSQW
                     QHFAIYDSRANPAGTNAPNPAHTDTNLNIRGNVIWNGDSGMVLGIEGNSDACIASNPT
                     CNETQLRADNAINTVEPLLVNPSAGDFHINGTWAAGVTTYDIPDFVWDIGGVPGGDNS
                     NAVPVDFEGVTRTGSNPPGAFFGGGSDTIFADVTSSYWAWPFIERLYNAGITGGCNTS
                     PLMYCPESSVTRAQMAIFILRGMHGSAYVPPAASGTVFADVPLGAFAVDWIEQLAAEG
                     VTAGCGGGNYCPDASITRAQMAIFLLRGEHGSAYSPPAGTGTVFSDVPAGAFAVDWIE
                     QLAAEGITSGCGGGNYCPDANVTRAEMAVFLVRAFSLP"
     gene            121292..121486
                     /locus_tag="PL000_00605"
     CDS             121292..121486
                     /locus_tag="PL000_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87421.1"
                     /translation="MKTRLLQSSSKMGLKKPFIFSVGEPKKIVRRKNFSEFTLLQTNK
                     IKISLQTRVVIETRFTTQGG"
     gene            121488..123755
                     /locus_tag="PL000_00610"
     CDS             121488..123755
                     /locus_tag="PL000_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC87422.1"
                     /translation="MYKTRSMHLFPKVLPSIALTVLLFIPFLSGSVEAVRALGGGAEP
                     NQSDGDTLYYFAEGNRIPLTPSLRWISVKFSESLSDQTAALENFAPMVGSLDQARPIL
                     FGDITLLPLNANMNVQSVLAGVNAMRADTAKFSQVYPVFQTADAEMALTDEFIVTFPS
                     GFRMDDIHNVNAFHGVELVEPVLGQENTFVLRVASGVDVLAMANLYQESGVALHSAPN
                     FVRILTSKPAVGQIPPSIAPMTDVNDPLYANQWYLNNTQQFGPETKNDADIDAPEAWA
                     LTTGSSSIIIAVIDEGVDLMHEDYKSNLVVGYDATGLGSKGAPVADDAHGTNVSGLIA
                     ASANNGLGVAGVCPQCRIMPVRVAYGDKYGYWVTDDATIANGIAWAYQNGASILNNSW
                     GGGLESTAINTAIANARSFGRGGKGSVVVFAAGNNNMTVVYPANLENVITVSASNLCD
                     QRKQPMDDACNGNEYWWGSSYGNSVDVSAPGVLLYSTDITGAAGYDTGNYYAEMNGTS
                     GAAPIVSGVAGLILSLNPNLTADEVQNILQSTADDVNGDGYDVDMGYGRVNAYNAALA
                     ANASSITFTDVPNTYWAYDFIERLYNSGVTRGCSSDPLSYCPLAAVTRAQMAIFILRG
                     MHGDTYVPPTASGTVFTDVTAETFGAAWIEQFALEGITSGCGSGVFCPNDNVTRAQMA
                     IFLLKGKYGASYVPPAATGIFADVPVGSFAADWIEQLAAEGITSGCGGGNFCPDQNVT
                     RDQMAVFLVKAFNLP"
     gene            123965..>125787
                     /locus_tag="PL000_00615"
     CDS             123965..>125787
                     /locus_tag="PL000_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC87423.1"
                     /translation="MLYLNDFFKNGCHLRRTVMRNVQLKKMFSKLLSTIILATMICST
                     FLSGNIELVRALGGRDASSAQEDGNSLYYFANGNRIQLTPSLNWISVKFTSDDPVAQS
                     AAVGGFGAMFGPLDQARPILFGNITLLPLKANVTEQALMEGIQSLRADKENFSQVNPV
                     FQTPDAEMALTDEFIATFPSDFSMEDIDAVNAFHNVELIDAILGQDNTFLLRVAAGTD
                     VLAMANLYQQSGVAIHSAPNFVRIVKINPTEGQSGQHVGPMAGTNDTYYPSQWHLNNN
                     QQYGSGTTNDADIDAPEAWDRTTGNSSVVIAVIDEGVDLIHEDISPKLVAGYDATGLG
                     SGGGPSGDDAHGTNVGGLAAASSNNGFGVAGVCQKCSIMPVRIAYSDAFGFWVTNDAT
                     IANGITWAYQNGASILNNSWGGGLEATVINTAIANAKTLGRGGKGSVVIFSAGNSNSS
                     VSYPAYLDSVIAVAASNLCDQRKKPIADVCNGYEYWWGSNYGSPVDISAPGVWLYSTD
                     ITGAAGYASGNYFADMNGTSGAAPIVAGVAGLILSLNSNLTAIEVQTILQNTADDVNG
                     GGYDVEMGYGRVNAYRAVQAVTTSGYTISGDAGIGGATLTYT"
BASE COUNT        29355 a        31025 c        33378 g        31829 t
ORIGIN      
        1 tgcaagcttg tgaccttgag acccgcttta tgaatttgct gtgccacttt ttgcgcgcgg
       61 tatttggtgc gcgtgaacac caaaacggaa ttcgtgtcgg tgcgtttgag gagttccagc
      121 aaaagtgtgg atttcaaatg cggcgcaacg ggatacaaag cgtgtgtaac cgtgtgtgca
      181 ggcttgatga tgcccattgc aattttttgc ggttgtttca atgcctgttg tgcgagcagt
      241 tccacgtcgg atggaaaggt ggcagagaac aacatggtct gtcgtttttc gggtacggct
      301 tttacaatgc ggcgcacatc gggcaggaag cccatgtcga acattctatc ggcttcatca
      361 agcacgagga attcgatgtt cgccattttt gcatgaccct gtgcaatgag atcaagcaga
      421 cgccccgggc aggcaacgag gatctctgcg ccgttgcgca atgcctgaat ttgcggattc
      481 gctccgactc cgccgtaaat ggttgcgctc ttcaactttg tccccgctga gaggactttg
      541 atgacatcgt tgatctgttc tgccaactca cgggtggggg tgacgatcag tgcgcgcgct
      601 gtgttgcgct gccctgaaag cagtttgttg agaatgggca gcacaaaggc ggcggttttg
      661 cctgtgcctg tttgtgctgt gccgatgatg tcccgtccgc gcagggcggc ggggatggcg
      721 gcttcttgaa tgggcgtggc tttttcgtag cccgcttttt ggattccctg catgaggcgg
      781 gaatcgagtt gaaattgatt aaagtccata tctttcctta ttttattaaa accacaaagc
      841 acatgaccca ttagggcctt gagttcctgg ggagttgggt gagcgcgccg cacctaattt
      901 gatgagaaaa ggctgctcat caaaaagaat ccgcccgtag ggtgcgacgc aggattcgcg
      961 aaaatagcaa gtttgctatc ggttcattat atccttgata ttgtgttttg ggcttgttta
     1021 agcctgaatg caatgatctt acgctaaaaa taacaccgct acgccgagga tggcgagaaa
     1081 tgtccctgcg ataccctgcc agccgatctt ttccttgaaa acaaaataac tgatcggcag
     1141 cacgatcaca ggcggaagcg ccatcagcgt actggcaacg ccgatctctg catgttgcac
     1201 agccaataat gaagaagaga cccccaacac agggccgatg accgcgccga aggcgatcca
     1261 ttgcagggct ttgggatgat tgcgaatcgt ttcgaccgtt gagctgatct gcccttcgac
     1321 ggctgtccat gcccatgtga aaatgaccgc cgccaacatg cgaatggcat tggcctgaaa
     1381 tggagaaaat cccccagcca tggcctgttt cgaaaggaca agccccacgg cctgccccag
     1441 ccctgccagt atgccaaaga tgattccctc gcgagtgtgt cctttgggag tattggcttc
     1501 ttctttgtgc gaggtcacca cccagccgat gccagccagc gcaagcagaa tgccgaggat
     1561 ctgcaaagcg gtcagcgttt caccgaaaaa tatccatgcg atgaccgaac cgaaaatcgg
     1621 cgcaaggctc aagagcaggc ttcccaaacg cggaccgact gccacatacg acctgaacaa
     1681 aaaggcatcg cccaatgaaa gcccgatcac tcctgaaaga ctcaaccaca accagcgcgg
     1741 cgagccagcc gagaaaggca atggttcatg gaaaaagatc aagttgagga tgacaagata
     1801 gaacagcgca aacagaagcc gaaagcgatt ggtgacttgt gaaccaacca tgcgcccgac
     1861 ggatgcaaaa atgatcgctg tgatggcaaa gaaaaatgag gttgccagtc ctgcgatttc
     1921 accgatgaaa ttcatttttt atacacctgg ttgattgccg ctatctttcc cgagttcggc
     1981 cctggtcaac agcatgacgg ccactagaat caatccgccg ccgagcatga cgatggggag
     2041 cagtttatcg ccgaacaacc atgcagccag caaaaccgta acaatgggtt caagcgtgga
     2101 aagcatcgcg gcattggtgg gaccaatgcg ctccagccca gccaaaaatg tgaccactgg
     2161 aatgatggtg gagatgacga taattcccag catggcgaac cagcccatgt tgttcactgg
     2221 gaaatgcgtt ccttttgaaa aggccagtat cccaaagact gcgcccgccg aagcgaagat
     2281 caccagcgaa gattgcaccg cagtgacatg tttcatcaca tttgtgccaa cgatgatgta
     2341 aatggaatag atcaacgctg cggtgatcgc catcaatgcg ccgatgatct gtccgctgac
     2401 ggggccgacg gtgagggccg tgccaaagag tgccagcacc agcgcgatga tttttacgcg
     2461 ggtcagggac tcgtgcagaa aaatcatcga aagcacagca acaaaaaaag ggtagagata
     2521 gaggagcaag gccaccaaac ccgccgaagc gtagttgatg gcggtcatgt acatgaaaga
     2581 ttgccccaca taaccgagcg cgcccatgcc gatcagttgg gcgaggatct ttccgcgtgg
     2641 aagatgttcc ttgcgcagaa gcaggatcac ggtcatgaaa gaggcggaga ttccaaagcg
     2701 caggaaaagg acagtgaagg tgtccatccc gttttcgtag gcgtatctgc cgaagatggc
     2761 gagggtccca aacgaagcag cggagatggc gatgaggagg atgccgatga gttttctcat
     2821 ggtgggaaat ataccgcact cggtctcaac aataaatccc tccgcgtgga gggatttatc
     2881 ttgaaatcgt ttcctgccgt gcttcgcttt gcctgtgaga aaaaccgtcc caaatttcct
     2941 gcggataaat gatctggttt gtgcctggca gttgttccat ggcgagacct gtgttcaggt
     3001 caatgcctat tttttggtag gccttgtagg ccaattgact gcaataaaaa gtttcttcag
     3061 tttcagggtt gaggaaattc aggttgtaag gtttgccgag ttgggcgagt acatagtctg
     3121 caacggcttc gcgcatgagt tgttcatctt cgtcggagaa gccgagtaca tcaagcggcg
     3181 cggcaatgcc ataaaacgta tcgaacaaaa gtccgcgctg gcgggccatg cgttcggtat
     3241 cccaatgtcc attgggcaca tcaaaagtga tcacgccgcg cgcgcccgct tcggcacagc
     3301 gaccatcagg gccaagataa atggcaacat ggtctacatc gcctggtaca agccgcccga
     3361 tcagccgcca gaattcgcct ggcagaatat cgggcttgcc gttcatcgtg cagatgatat
     3421 cgcctgtttg taaagtcaat ccgttgatct ggtaagtgtg tatcatttcc atgttcatta
     3481 tacggaaatt ccctcccaaa cgtttcagcc attcatcctg aaaacgaaag caaaaattca
     3541 cggatctgat gcgcggtctg attccatttg ggctgcacca gataacgatt ccgcgccgca
     3601 agactcattt ggatgaggag atcgcgcttc tcgttcataa gctgtagcct ggctgccaac
     3661 gcgtgagagt ctcctggctg aatcagatac cccgtcacac cctcttcgat gacttcgcct
     3721 gccgcgccca atgtggtgcc gatggcaggc aggccaaacc ccatcccttc gagatagacg
     3781 atgccaaagc cttcataact tgaagggaca acgagaatat gagcggcttt gagcctttcc
     3841 accagcggtt ggttgttgag tgcgccatga aaatgaacag cagaagatag gccgttggtc
     3901 gagacgaact cctgcatttg ggcggcgtat tcgggttcgg tggcaagcga accaacgatg
     3961 tcgagtcgaa ggtcaaggtt cgaaagtcgg actgcttcga gaagagtgtg aagccctttt
     4021 ctccccatca catttccgag gaagagcata tgcaattctt tcttcttact tctttccttt
     4081 atttcctctt ccgaaattgc atccccaaac ctgtctgtgg gcgggtaggc aacgacgtca
     4141 ggcttgccgc ttccaattaa atcgttcacc acgccttttg tggtttttga attgaagata
     4201 aacccatcca cgctttgcag atatcgcttt tcgatgggac gatataaatc gttctgccat
     4261 tttgggcgca attcagaaca gcgcaggtga tggacgagcg aaataatggg gtagggatgc
     4321 cgcccgtggt ttgcgccgat gagcgatgga tgattgagct catcttgaat caaaatatcg
     4381 agatttggcg gcagtttgaa agtgaggtta tctgtcaggt gcgcggcgta attccgccac
     4441 ggcaacgaga tgatctccac tgaatcacct tgtgcgcgca gatattccac caacatgcgg
     4501 tcgtacatat atccgccgct gagagtgtca atggagccgt agatgacgag tccgattttc
     4561 atttataaga gttgaaaggt tgtaaagttt gaaagttgga aggttgaaac tttctacctt
     4621 gccaaccttt caacggaaaa agcggcccat gcattttcat gttcccacag cactaccttg
     4681 agcttggaaa tgttcctggc cttgatgcgc tcgttcaacg tctctgccag aatgcgtgaa
     4741 aaatgttcaa tggatggatt caaacctgca aactcaggtt tctcatttag catctgctct
     4801 ttgtaataac ccaccacatc atcaaggtgt ttttccacat ccacaatgtc gacgagatag
     4861 ccgtgttgat cgagttcact gcccttgagt tgcaattcaa ggatgtaatg gtgcgaattt
     4921 ggaaaattct ctggccccca atctccgccg atcaaaaagt gacgtgcaat gaattctctt
     4981 cttactccaa gggtgtacat aaaatgctcc tgtgttggtg tcaggtgtca attttcgggt
     5041 tttaggttga gcgcctgcat gatcaaaaat ccattttttc gttcaatggg catggcaaag
     5101 ttaaaagcgt tgatgcgtgt gcaaaggtca agatcagatc ttgggaattg aggatgatca
     5161 ttcatgtgct ggaatgcatc tcgcgattgc accacgcggc ggatcagttc ttccgaatcc
     5221 actgcctgac ctgtgaactg tttttcaaaa aattcagcac aggccaaatc ctctgcattg
     5281 tccgctccgc ctgtgacaac gaaagtgacc tcttctggcc gcaatcgcaa gacatgctcc
     5341 atcgttgcct gtgccacaac aaaactggcg gcaaacatta cttccgcatg gacgctgcga
     5401 accgcgcctt gtgtacctgc gcctgtgcgt tgaatcaggg taattccagt cagatcatgt
     5461 tcgaggatgc gagtggggga gttcccaaaa tcgaagcctg caggcggcaa cccgcctact
     5521 tcgcccatca cttttgaatt tgggagttgg gctttcaact ccaatgcttc ttccactgcg
     5581 ctgaccagtc gaatctccct ggctccgcgt gaaaaggcgt atgccgctgt agagaacgcc
     5641 cgcaatacat caatgacaag gacgatgccc gtggcggtat ggcaggtttc gagagtggtg
     5701 taattgaatt tcattttaaa taatcaaaca caacctgaat ggtttcctgc ggtttttcat
     5761 ccagtagttg ataggctttt gcggcatctg caattgagaa gcgttgtgtg atccattttt
     5821 cggggcgaat tcgttcgaga gcatgccatg ccacatcaaa gcggcgggat ttatcccagc
     5881 gccccgatag gtctggtgag atcgtactga cttgtgacga gatcaatcta atacgcgaac
     5941 ggtgaaatga ccccccaaag tcgatctctg cccgcttttg tccataccat gacccgatga
     6001 caatccgccc gctgaagatg gtcaactcaa tggcatcgtt caatgcagaa ggggagccgc
     6061 tcagttccag ggtcagatcg aacttatttt gcgtataggc aattgaatcg acgtggaagg
     6121 ttttgaggct ggaatccaga gaatgcacac ctttaatctg cattgccgcc ttgcggcgca
     6181 gttcataaaa gtctgcggtg agcaggcttt cgagtggaaa ttcacttaat aaagaagagg
     6241 tcaataaacc aacgacaccc tgtcccaaca ccaggacgcg ctcacccaaa ataggggcag
     6301 cgtcctgcac caggtttact gcggtttcca tgttagggag gaaacaggca ttttcagagg
     6361 ttagggaatc aggaatggga atcaaagatg aaggattaat aatgaaggat gaggcatgcg
     6421 gatgaaatgc aaagacatgc ttgtctttcc acgctcggct tacatccgcc ccaatttcct
     6481 ttactttccc cacacatgca tacccatagg ccagcggata attcaagtcg ctgctgaaag
     6541 tgtcgtgaga atcggccaaa tgagggaatt gcccacgata gaccagcatc tcggtccctg
     6601 cactgatggc ggagcagatc gtttccacga ggacttcatc ttttttcagt gggggaaggg
     6661 tgatctcttt aatttcaact tgtttcgggg ctgtgaaaaa aattgtctgt gcttttggca
     6721 tgggcagagt ttaacataaa aatgacagcc gctttatgag gcggctgtca ttgtgtgctc
     6781 ggtttatttg ttgggcacgg gtttggtcag gtaactgggc aggtcacgac gaaactcgta
     6841 tttttccatg atctcttcaa tgacgggctt ttgtttgggt gtgacttcct tgaattcgaa
     6901 ccctacattg aaatattcgg ggctgatatc ttgctgacac caggccgccc gtgcagggat
     6961 ggtaatacgg gggatcttca cattttccaa ttcaggcagt tcgatggcga gggtcaattc
     7021 ggtgttttct gcaatgggct tttcgccgat caccatggca ccatgaaggt ttaggtcacc
     7081 cagatagcca agtaaatgcc cgccattcaa atcgaagacc tgggtgtaag ccatcaaatt
     7141 cttgcgctgt tgtttgcgtc tttcctgcat tggacctctc acatgggcgt gggtatcttt
     7201 tttattctac aatacatcat cacgggattc attgcgattt tgtggtaaaa ctggcggatg
     7261 cgcctcattt atatctcccc ccatctcgac gatgccattc tttctgctgg cggcctgatc
     7321 cacgatcagg tgcaagctgg caggcatgtg gaaatctgga cggtcttttg cggttttccg
     7381 cctgccaatc aactttcctc cttcgcacag attattcacc aaaaatgggg catcatggat
     7441 acgaatgaac tggtcgctgc acgccgtgcc gaagacaagg ccgcggcgga tattgccggt
     7501 gcgcaggtat ttcatttcga ttttctcgat tgcatctatc ggcgcggcag ggatgggaac
     7561 tggctgtatt cggatgtatt cattcctcca cataccgatg atgacggcct gccggcgcag
     7621 attgcagaag ccatttctgc ccgcctcaac cctgacgatg aactggtctg ccaacttgcg
     7681 ctgggtgccc accttgacca taccctcgtc cgccgcgcgg ctgagttgct tggccgccca
     7741 ctgacctatg acgtggatat tccatacctt ttcaacttcc ccgatgattt tgcgttgaaa
     7801 acggccaaaa tgaaggaaat cactcacagg ataaccgaag caggcctacg gtcatgggga
     7861 gatgcgatcc ttgcgtatac ctctcaattc ggcaccctgt tttccagtcc cgaagagatg
     7921 cgagaaaagc ttcagcagta ttgggctgaa aagcaaggta tttccttctg gaaatttgag
     7981 tagacaaatt tactggtgct tttggggctt gtaacccctt ttaatctatg atatagtatt
     8041 gacgaatttc cggagatgaa accggaaatt tttatccatt gcatcccttt tggggtgcaa
     8101 aattctatat agcaggacaa accatgccaa caaccaattt ccttaccaaa gagggctttc
     8161 agaagcttca agaggaactg gatcatttgc gcacagtgaa acgacaggaa gtagccgatc
     8221 gcttgcacga ggctatggaa ggcggggaac tgatcgaaaa tgcagaatac gaggcggcca
     8281 agaacgaaca ggcttttgta gaaggccgta ttcaggaact tgaaattttg cttgccaccg
     8341 ccaacattat cgaagaaaac aacggcaaag ccaagaaaaa tgatttgatt cacgtcggtt
     8401 caaaggtgac cattaaagag gggaactttg aagccgagac ctttaccatt gtgggggcgg
     8461 ctgaagccaa cccacgcgag ggaaagattt cgaatgagtc gccgattggc aaggccattc
     8521 tcaatcacaa gctgggtgat atcgtgaaag tggaaacccc tggcggtact tacaacgtga
     8581 agatcatcaa ggtcggctaa acaggacgcg caacgcttaa cttgcccgcg tccaatttgc
     8641 gttggatgcg ggctttttat ttgaaaaata ctttcttgaa aacaggaagg aagaagaaaa
     8701 tcatggctga atacaactca ctggaaaaaa tccgtttgca gaaaattgaa gaactccgcg
     8761 ccgagggcat cgagtcctac cctacacgcg ccgagcggac tcataccaat gcgaaggtca
     8821 ttgccgagtt cgaggccgct gaaaagaatg ccgctcccga tacgaccccc gaagtgaagg
     8881 ccgtgttggt ggggcgtatt cgtgccaatc gcgcaatggg gaaggtttct tttgcgcaca
     8941 ttgaagatgg cagcggcaag atccagcttt tctttcgcag caatgaattg ggcaaggaac
     9001 gccttgacct gttcaacaaa atgtttgacc tgggagattt tatacaggca tcaggagtca
     9061 tgttccgcac caaaacaggt gaggtctcac tgcatgtcca tgacttcaaa ttgcttgcca
     9121 aatctgtaac gcccctgccc gctgacaagg atgtcaccca ggaggatggt acggtaaccc
     9181 gctacgccgc tttggaatca cctgagctgc gtgcccgcca gcgttatgct gacctggctg
     9241 ttaaccctga tgtgcgcgag accttccgca aacgggccac gttgatcaag gctttgagga
     9301 atttcctcga cggtcatgat tttcttgagg tggaaacgcc catcttgcag cctttgtatg
     9361 gcggggcggc tgcacgtcca tttgtgacgc atcataatga actcgaccag gatatgtacc
     9421 tgaggatttc gttcgagttg tacctcaaac gcctgctggt ggggaatttg gagcgtgtgt
     9481 acgagatcgg gcgcgatttc cgtaatgaag gcgtgtcttt caaacataac cctgagttca
     9541 cccagcttga gttctactgg gcttatgccg attatttgca ggtcatggag ttgactgagc
     9601 agatggttgc ttatgtggcc gagcaggtga caggctccac caaggtgacc ttcggcgagt
     9661 atgagttgga attcaaaccg ccttggaatc gtgtggaaat gcgacagggt attcttgaaa
     9721 ccagcggcat tgacattgcc gaacacaaaa cggctgagga gcttttcaag gccattcgag
     9781 ccaaacatcc gaacaaaacg cccgatccaa aatcggcgcg cggcaagatg atcgattttc
     9841 tgctgggtga gttccttgag ccgaccttga tccagccaac attcctctac aactatccac
     9901 gcgatatttc gccgctggca aaatccattc ctggtgaccc gctgaccgtg gagcgttttg
     9961 aaggatatgc ggctgggttt gagttgtgca atgcctttac tgaattgaac gatccgcttg
    10021 atcaagagca gcggtttatt gaaatgggcc gtgattacac ggatgatgaa gaagagaaac
    10081 acccgcttga tgaagattat ctgcgcgcca tgcggtatgg catgcctccc aatggcggtt
    10141 tcggcatggg tgtggaccgt cttgccatgc tgttgacaaa taaacactcc atccgtgagg
    10201 tgctgctgtt ccctgctttg cgcaaggaag agtgaggtct ttgtgcatta catttctccc
    10261 gataaggaag aacagatcag gcagatcgcc cgaagcggca atctgatcga ggcgatcaaa
    10321 gaatatcgcc tgttgaccaa cgcaagcttg aaagatgcca agagtgccgt ggaggcgatg
    10381 gtgcgtggaa attatgtgaa tggccccgca cctcaagctg ttgccaacgg cgccgattca
    10441 tctcaaaaaa gccagatctt ggatttaatg tccaagggtc gggagattga ggcgatcaaa
    10501 ctctatcgcg gttggaccaa ggctggcctg aaagaagcca aggatgcagt tgaggccatg
    10561 gcacggggag aggatgtcca gatccctgag ccagtgacca gcgtggagag tggttcgatg
    10621 gatggtcaga tccgcgcttt gttggcgaag aatcaaaaaa ttgaggcgat caaggtacat
    10681 cgtgctgcca ccaattccag tttgaaagaa gccaaagact acgtggaagc catcgaaagg
    10741 ggagtgcggc caaacccatc atctcccctg aatattcccc tcagcaatga cccgtttgaa
    10801 gaggagaatg ttcgtacgcg ccgcatgatg gtctttgtat tcatcatatt ggcaattctg
    10861 gccgttgttt tcgcattcgc ctttttgagg ggtggtttat aggagcatcc atgcaaaagt
    10921 caaaactcgt tcttatattt ggcgatgtga tcgccattgc cctgcttacc ttcatcggtt
    10981 ttgccacaca tggcgaagcc gatgtttcct ttttgccgcg catggcggcg gcattctttc
    11041 cagtattaat cggttggttc gtgctggcgc cgtggtttgg tttgtttgat gagcagatta
    11101 tttccaaccc caggtcgttg tggcggatcc tgcctgccat gctggctgtg gcaccattgg
    11161 ccgtgatgtt gcgcgccgcc attttgaatg ccgccgcttt gccgcttttc accctagtgc
    11221 ttgggggtac caatgcattt ggcatgttgg tgtggcgtgc attgcatctc tttgttgtaa
    11281 aacgaaatgc acgctgattc aataaatcgg caagtcgacg attcgcgggt aaaataaact
    11341 catggatgaa ccagccctta ttcagaccgc acaaaatggg gatcttgatt ctttcaatac
    11401 cctgatcctg cattaccaag accgtgttta caacaccgcc ctgcgcatcc ttggcgatga
    11461 agaccaggct gccgatgcgg cccaggaagc ttttatttcc gcgtttcgca gcatcaattc
    11521 gtttcgcggc ggatcgttca aggcatggct catgcgcact gtcaccaacg cctgctacga
    11581 cgagttgcgc cgccagaaac gacgccccac aaccccactt gaacctgata ccgccgacgg
    11641 cgaggagatg gattctccac gctggctggc tgaccccaac atgacccctg ctgagaaatc
    11701 ggaggcggac gaactcgagc acgccattca gcattgcctt gatgcgcttc caacagactt
    11761 tcgtacggtg gttgtcatgg cagacttgca aggccttgat tacaccgagg tggcagccgc
    11821 tgtacgcgtg ccattgggga ccatcaagag tcgtctggca cgtgcgcgtt tgcgattgcg
    11881 tgagtgtctg cgtggcttcg aggaactttt gccttcttca tttcgtctgg aagaggaaag
    11941 tagattatga aaaacttccg cgatattgaa caactctctt cctatctgga tggacaactc
    12001 agcccatctg attctgcacg cgttgagtcg cgcatcgcat cggaccctga gcttgcctct
    12061 gccttcgaag acttgcgcgc cacccgcggc attctgcgta aactgcctgc acgcaaggct
    12121 ccccgcaact ttaccttgac ccgtcagatg gttgggttga aaccacccgt ctcgagatcg
    12181 tattcctttt tccgcttttc aagtgccttc gccactgttt tattgatgct gacctttgcc
    12241 gcgaattacg ttgctccgcg catgtccgtt gtgcctgttg gtggttcagg cggtggttgt
    12301 gacccctgcg gccttggcgg cggtggcggc cccgaatcct ttaatatgga tgccctacct
    12361 gccgcaacag aagctccagc ggttgcggcg gagtcatttc tcgctacaga agcccccgct
    12421 gcaactgaag cgccagccat ggtggaggaa ccagctgcta ccgaggcgcc cgtggaggtc
    12481 cttccaacca tggatatggc tctggccccg acagaattac cgctctccac gcaagacaca
    12541 cagcgaatcg ttgaaacgcc ttccgcttct cccaaagaac cggtgattga gtcagcaccg
    12601 caacagcagc caccggtaga ggatgaatcg tttatttctg ccacatggca ggtggttttg
    12661 ctggtcatcg gtctggtcag tggattggcg gccttcttca tgaatcgttc cgcaaagaaa
    12721 aagtggagtt aaacattggc tcgagatagc catattaaat tctgcccgcg ttgcggggct
    12781 gaggttgcac acgaagaaaa gtttggaatg gtgcgcccga tctgccctca atgcgggtgg
    12841 atttatttta ttgacccgaa ggtggcggcg gcgattcttg tagagcagga aggccgtgtg
    12901 ctgcttgtgc ggcgtgttaa cgagcctttc cgcgggttgt ggactttgcc cgcagggttt
    12961 gtgaacggtg gtgaagaccc agccaaggct gctgcacgcg agtgcctgga agagacaggt
    13021 ttacacgtgc atgtgaccgg tgttttggat gtgattgctg gccgcgagca cgaacgtggc
    13081 gcagatttca tcatcgtgta tcatgcggaa gtgacgggtg gtgagttggt gcctgctgat
    13141 gatgctgatg cggcggagtg gtttttgcgg agcgaattgc cgccgctggc tttcaatgcc
    13201 accaaaaaga ttttggaatg atcatgaatg cccaagacta cggttgggcg aaaaacttga
    13261 taacaaaaaa gacttcggag ttttatctcc gaagtctttt ttgttgagga tgattctgct
    13321 gctgatcaag ccgctgttct cggcggcggg gacgccgccg agcgcattgt ttatttcgac
    13381 cgactactta gctcagaagt ctttttatgg tgcgttttgg taggtgatgt attcgtagct
    13441 tgaggtgatg ttgttgtcaa aatccacggc gtagaagccg atggaatatt gaccatctac
    13501 agggatatag gattcgaacc agaagccttg ttcgccgtag gtgaagacgt catcacttag
    13561 ttcattggtg taagtggcca cgccattcgc atcgaaggtg tagtattgaa cgtaatcggt
    13621 gaagcggtcg ccgatctgcg gggagatggc tacagcggtg tcaatgccgt tgctgtctga
    13681 gtcgggcagg gcgtaaatgt tgagcaggtt gccatcgagg tcgaaggtga gtttggcatc
    13741 ggtgggtgtg gtggcattga ccggggtgta ttggccgtat accgagtagg tggtgacgcc
    13801 atcggcgctt tcgtattcat cgggttcgaa cagggcatag gcctgattcg ttccgtcttt
    13861 gaggacgaag agatagggtt cccattcgta actgatctcg atggggcttt cgccgtaatc
    13921 gggggcattc actccgtcca cgctgatggt gttgggcgca ctgacaaagg acatatcggc
    13981 cagccaggtg acgttggcat cctgattgta aaagaagagt acgaagtaga tgtaggaaac
    14041 atcaccgctg actgtggtgg agagggtgag ggtttcgtcg gcggagatca cagtgtcaga
    14101 aagctgaatc ggggcaatgg tcaggggaga tgcgcccggg gcattgatct cattggtgcg
    14161 cgggggagtg taggcttgtc cttcctgggg agtaaattcc tgcccggtgt agtggtaagc
    14221 gaggaattca tcccatgtgg agagttgcag gaagcgggcc gcggattcgg cgtagtaggg
    14281 tgggaaatcg ctgttgtatt cggtgtaggc gtagatatca gagatgggga agtggaagga
    14341 aatgccgttc gagccagaca ttctttgccc gtgtttttca gcaacgaccg ccgatgaaat
    14401 ggcggctttc agttgttcag aggcttggaa tatggctggg tcgccggtgg cattggcgag
    14461 gatttcggag aaattgccga ggtcaataaa tggggaaggc gtatcttcgc cgaagatgct
    14521 gtaatagctg cgggtatatt cgcgagcctg ggctacgtac cgttggtcaa tggtggccat
    14581 gaccgagatg aattggttca ttgcgccgat gacatcaggg atgcgtgcgc tttccactgc
    14641 gctcagggtg gtggcggctt ccacttcttc gatctcagaa ctgctggttg acctgagggt
    14701 gagcagggta tcctgtgtga tgtaggtgga gatgatcgcc tggctgaccc cgcgtccatc
    14761 catgctgggg ttttgtgcca gttgtcccat ccatgcggcg tatgaccagc cggtggaggg
    14821 aattacttct tcggaggcga tcatgtaatt ggagtaggga tagagcgacc catatacttc
    14881 caccatgccc atcaaacagg catcgaaacc gatgatctcg aatttttgtc cgcccatttg
    14941 ctgttgaacc acttcgatgg cctgaacaat ttgcggcatg gaaagactcg aacccgattg
    15001 caggtctgag aagccgccgg tccagcctcc accgtggtca gacatgatga gggcgtattt
    15061 cttggctggg tagttttgaa tggtccaggt catgaaatcg acaagggttt gcgggttgcc
    15121 catgtccact tcgccgaggt tttgcaccac cggtgagttg atctggttga ggtcggagtc
    15181 ctgggtgatc aagaggcggc gggcatctgt ccagtcacca tcgccatcga aagagccttc
    15241 ggcgcggtcg atctgcacga cgatgttcat ttgcgtattt gagccgacga gctccatttc
    15301 gttgatgtcg aaccacaggt cgccttcaag gatgtcatca tcggcatcgg aatagtagat
    15361 gatcgtccat tcggggaggt tggtggtgtc ggcagggatg atggggtcgg tggagacgac
    15421 aggcggtgtg gtgacacttg tgccgccaaa gatggaatct ccgtaaatgt ataacaggac
    15481 gcctgcacaa catacgcagg caatgcagat gacaaccgct gcaatgatca cggggatgcc
    15541 aatttttgaa gttgtgtttc ccatatctcc tccggtaact gttttatttt tagtttctgt
    15601 tccagcataa tagcacgata agacaagcca atgcaagagg gttttgatgt tatatgacca
    15661 aagtaccttt cgatttgaac attttcgggg tatccttgcg gtcaactttc aagaggagat
    15721 aaggagaatg aacatgaaaa agcaataccg tatgttgttt gcctttgccg ccctgttgct
    15781 gttggtcagc attgcgtgca atggcggtgc ggctcccact gctgcaccgg ttcccacact
    15841 tccacccgct cccaccagtc agccccaatc caataatccg ccttccaata acaatcccat
    15901 cgtgccgcag cccccggcgt tgaatgaacc cccgccttcg aatggcaata gctcggacct
    15961 ggttgtgttc accgatgaaa acaacctgct gtcatttgaa gttcctggtg actggtacta
    16021 tgaacagcag acttatgaac atgcctacat tgaccgtatc acttccacag atggttccgc
    16081 cattattgac agccttgtct ataacgatgg tcaggctttc acaggaaatg acaatggccg
    16141 ctttgccttg cagttgctgc accaatatta cagcaacacc ggcaaggaag gcgatatcag
    16201 catttccggc gatgagatgc agccagatgg ctctgaaaaa ttgacctgga cttcgaagag
    16261 cggaggttat tccggcgtat ccttctttga actgcgcggc ggtgatcgca tgaccttcct
    16321 gatgttcacc ctttattatg ccaatgatgc agatcaagca gtgatcgaca tcctggatca
    16381 tgccatcacg agctatacga tcccatagtt gaatgtattg attaatggaa acaaaaagag
    16441 gctggagaaa tccagcctct ttttcatttt ttctttttat tcggggatga acaggaacat
    16501 atctgtcatt tcgcagcgtg tgccgtagcc gctgttggtg ccagagagca gggtcaacgc
    16561 aaattgaccg ttggtgccct gatctgcaga cagggtgtat tctgtcactt caccatccac
    16621 agagacaaat gcgctcttgc ctctcaccgc cagcgagaaa tctgcttcgg cggggtttcc
    16681 aaagttcaca cgcccggggc cgcgggtttt gccaacttca taaacgcttg agccacggct
    16741 catgaagaat gcgatgcggc ctttatcgag gaagaccaca tagtgatcgt cactgccttc
    16801 ttgaaggcca aagacaacgc cgcagcctga aaggtctgga gtggaattcg aggaagacca
    16861 cttgaagtgt ccggagaata cgaggtcaga cgcttcttta tcgatggtcc accattgata
    16921 gtagttgagt tgtgcccatt cttcttcaaa cggatcgatc ggagtgattt caccctcggt
    16981 ggtgctgaca tagccctcat cggcaaattc ctgcaattga gtttcaaatg cgctggtgtc
    17041 caccgatggc acgacctctg ccggggtggg ggatgcttcc accacgggcg gcgcttcagt
    17101 gggagggatt gcggttggcg gtgggttggt cggcggaagc ggggttggag ttgcatccgc
    17161 cttgccaaaa ttacaggcaa gcatggcgag gatcaatgac gaaattgcaa gtacgcttga
    17221 aagatttttg cttgttttca tggctcactc ctttttttga tttttttatt atatcagtgt
    17281 catacaggga caatataatc ccccaattgt acacaaggca tgttgtagaa aataagaact
    17341 tcctttgcat aattgacctg gtactgtacg aattttgacc cactccttgt gctacaatga
    17401 atctgatgac cacacttgac cttcgaccct ctccgattgc tggccaatgg tatgacgcga
    17461 atcccaccgc ccttgcccgc agcgtggatg attttctggc tcatgcgagt gtgccccaat
    17521 taaatgggga agtgctggca ctcatcgcac cacatgcagg gcacctctac tctggagcag
    17581 ttgccgcgca tgcctttgcc accctgcatg gacttgcccc tgaattggtc gctgtgcttt
    17641 ctccatatca caactacgca cattttccac tcatcaccac aaagcatcag gcttatttca
    17701 ctccacttgg aaacctgtca gtcgatcagg ctgcgcttgc agagttgcag acccacctcg
    17761 agattcccat cacagccatt gcaaatgatc gggaacattc gctcgaaatc gaacttccat
    17821 ttttacagcg ggtgctcaaa aacgaattca aattgctgcc gatcatgatc cgtgcgcagg
    17881 atgtgaccat tgcacaaaaa ctcggtcacg ccctcgccca aacgctcaaa gggaaaaacg
    17941 ccatcatcgt agcctccaca gacctttccc atttttacaa ccagaaaacc gcaggcattc
    18001 tcgatgcgga aatgctcagg cgtttcgagt ctttcgaccc tgagtctgtt tttgatgccg
    18061 aacaaacggg agtggggttc gcctgcggac atgccgcagt tgcctccatg ttgtatgccg
    18121 ctcgcgaact cggcgcagac aaagtgcaga tcctgaaata tgccacctcg ggtgatgtca
    18181 ctggggatac ttcatcggtc gtggggtatg gcgcaggagt tgtgttgcgc ccgtcaactt
    18241 cccactgagt tctgatcact ttttcccgtt cactgaatca tctcaaccgt tcccactctt
    18301 tcactgcatt tcgaaatgca ttcttcaagc cttcatcggg caggccatcc acgctttgat
    18361 acatgctgcc gttcacccaa atttccagtg aaccatcggg cgcagaacca aaatcaatat
    18421 caaacttgtt gaaagggtca ctttccaatt tggcgcgcag aatatcctcg atctgttcgg
    18481 caaagggaga agcgatcttt tcgcccgcat cgtgatcgaa caaggtcacc ccttcacctt
    18541 cggcgcgtgt ggctttcaca gtttcttttg gtgggttcga tttcatccac tcaggcttgt
    18601 cttccgtttt ccccgtcagg tttacttcgt tcgattttat gaacataaac gccagcagta
    18661 ttaccgatgc caaaattgca atcccgccca ggatcatgat gattgtcaac atgtctgcct
    18721 ccttttcgac ctcatcaaca ttataatctc accatgtttg cacgtgtcgc cgttaacgtt
    18781 tctgtaatat caggcctctt tgattacgcc atccccaacg aacttgccct ccaaattcag
    18841 gcaggtagtt ttgtgatcgt cccatttgga aagcagacgg tgcaggggat cgtcaccgct
    18901 ttgatcgaca ctcctgcggt ggagaatccc aaacccatca tggaactgct cgaccctgcc
    18961 cccgttctga ctcaaccgca gcttgctctt gcgatccaat tggcagaatc aaccctgaat
    19021 ccactggccg ccatcatcag cctcatgctg cccgtgggcc tgagccagca agcggacgtc
    19081 cattacgaat tacggaatgc caattacgaa gacaagacct caaccgtcgc gactcgtttg
    19141 ctgacacttt tgcgtgagcg cggtcccctg cgcggacgtc agatcgattc tcatttctcg
    19201 cgggtggatt ggcgcaaaac ggcgcaggcc ctcgtgagaa aagggattct cgcaacgaag
    19261 cacgtgctgc ccccgcctcg ggtgagatca aagttcgtgc gcgtggccca gttgtcggtt
    19321 acgccagaag cggctgaagc ggaaatggat tccctcggca cgaagcagac tctcgcccgt
    19381 cgtcaatctg cattgcggta tctcatccag cagcctgaag cgatcaacct ttcgtgggtc
    19441 tatgccgaaa caggatgcaa cctcgccgac ttgcaggaac tcgaagagcg cggattgatt
    19501 cgcctgtttg aaaatgagat ctttcgcgat tctctgcaga gaattgaaaa tggaaaagaa
    19561 gaacacggca gacaatttga actgacctca gagcaaagtt catccctcga aaaaataacg
    19621 cattcatcct tcatccttca gccttcaccc tttcttcttc atggagtgac gggttctggc
    19681 aaaactgaaa tctatcttcg tgccgccgaa gaaaccatca agcgcggaaa gcaggccatc
    19741 atcctcgtgc ctgaaattgc gctcactccg caagcggtgc gcagattttt agcgcgcttc
    19801 cccgggcagg tgggcttgat ccattcgaaa ctttcagaag gggagcgcta cgatacgtgg
    19861 cggcgcgcgc gttcgggcaa gttgaaggtc atcattggag cgcgttcagc tttgtttgcc
    19921 ccgctgccaa atatcggctt gatcgtagtg gacgaatgcc acgacagttc ctatcaccag
    19981 actgagccgc cattctataa cgccgcagtt gccgcgcagg aatatgcgcg tttgtgcggt
    20041 gcggtgtgtg tgctgggctc cgccacgccc accgtggagc aaagattcgc cgccgaaaac
    20101 ggcacgctga caaaattgac tctgccgaat cgcgtcaccg attctgcatt gccgcccgtg
    20161 caagtggtgg acatgcgcga ggaactcaag acaggcaatc gcggaatctt ttcgcgcgcg
    20221 ctggccgaat cactcgctga aacgatctca cgcggcgagc aggcgatttt gtttttgaac
    20281 cgccgcggta ccgccactta cattttctgc cgcgattgcg ggttcgtgct caaatgcccc
    20341 aactgcgata cgccgctgac gttgcacgtt ggaacgtttg aacgttcaaa cgttccaacg
    20401 ttactttgtc atcattgtgg ttacacccgc caaaagccga agacctgccc gaattgcaat
    20461 ggaaaacaga tccgcgagta tgggcttggc agtgaaaaag tggaagagga agtgcaggcc
    20521 atgttccccg gagtccgcac cctgcgttgg gactgggaca cgacccgtca caaggacgca
    20581 catgaaatga tcctcacaca tttttccaat catcaggccg atgtgctggt ggggacgcaa
    20641 atgctctcga agggactcga cctgccgttg gtgactctcg tgggaatcgt gctggcagat
    20701 gtggggctga acctgcctga cccgtttgcc agtgaaaagg tctttcaaac gctgactcag
    20761 gttgctggcc gcgcggggcg ttcgtcgctg ggtgggaagg tggttttgca aaccttcatg
    20821 cccgagcatt acgccatcca atttgcggcg ggacatgacg tgaacggatt ctacgaacgc
    20881 gagttggact atcgcaagca gttgggctat ccgccttttg caaaactcgt gcggttggaa
    20941 ttccgcgatg ccgacccgct caaagccgaa tctgattcgc aaaaagccgc cgacaaactc
    21001 aaactccgca ttgaaaccga aaaccgccgc caaaccgagt tgatcggccc cgtccccgcc
    21061 ttcttctcca aagtggatgg aatccaccgc tggcagatca tcctgcgcgg cccagacccc
    21121 gcttcactgt tgcgcgatct aaaattgagc gattgcaggg tggaggttga tccgattagt
    21181 ttgttgtagt ttgggttgaa ggcaacggcc tgagtaatcg ggctatggag cgggggttga
    21241 actgagaacg atcaatggag gctcttctgg gtgctctatt ctccatgtaa ggtgatctga
    21301 taactgctga taattcatta agttattaag aaaaatttct tgtatctcta tggggaacat
    21361 ttcacgaata tcagaactcc aattcgttcc aggaatttcc accttttgta tagccccgtc
    21421 tacgccaagg tgaatcactg cgggagagtc tgatccatta tctaaaattc gacacgttgc
    21481 ccatacatat atttcgtttt cagaacgtcc taaaacctcc cattcgcata aagcaagttc
    21541 ataagacata atagtttttg ccagagcagt ttgatactct ctccaccgct ccacttgaat
    21601 tgaatctggg gtaatcatga gaatgatggg gtcagttggc actggggatg gagtttcagt
    21661 ggcaggaatt aaggttgatg ttggaattgg cgttgatgat aaaacgatca ttggaaggat
    21721 ggtttcagtt ggtgcagagg taattgctgg cgtacaagcc gtcaatacgc tgaccaaaaa
    21781 caatacaagc agtgcatttt tcatggcaaa ttccttcatt gtcgtatttt gatcgtggga
    21841 agttcttgct tttcccccac gcaatctgga ttatatgaaa aaacgcctcg cggtgaagtg
    21901 gcgaatgtca tggcttttgg ggataccacc cacaaacaaa aagagacagt aactcgcgcc
    21961 actgcctctt tcttctttcc tcttacttat tactttaccc caccaacccc accacaaaat
    22021 tcaacgcccg ccagtacagg ttatacaacg gcgcgggtaa attcttcgaa ggcaaaacag
    22081 gcggaaagga ggatgaagcg ttcggctcgt cgaagcgatt gtctgcgcct gtgccatccc
    22141 aaatgatatc gaagccctgt cccagcatgt ctttcacgaa tttatccgca tcgtagccgt
    22201 tgttttcgta gatattatcg tggatgtagt tatgctcggg gttcgggccg acatcgatct
    22261 cctcttccga aaaaccgatg gtcaaattga acacggccat gccgcccgtt ttatttccgc
    22321 ggatggtgtt gttgtacacc tccacatggt ccgctgcgag aatcaacata cccgtgccag
    22381 gcgggatgag cgaaaccgct gtgccaggct tgccaaagtt tgcgccgttg ttgttttcaa
    22441 ccacgttgtc atgcaccttg gtgtacatcg aaaccttgga gggcagttgg ggcagcaggt
    22501 caatgaaaat gccgatggtg ttatcgtggg ctacattgtt gtacacttca ccattgaccg
    22561 tgttttccaa ctccacgccg atcacgttgc cataggtcag cgtatcgcga atgatcacat
    22621 tctccgattt gcccgcatag attgcggcat cgttcatcag cgtgccttcg atgcgttcga
    22681 tcaacacatc ggtgcagcgc acggggtaca cgccgtacac gcctgtattt tcaatataca
    22741 tgtcgtgcag gtacacattc ttcgcgcctt ccaccagcac accattgctt gtgaaattct
    22801 tgaccgcaaa gaacgccatt tcaaaattat tccccgaggc gatcacccca tctgcaccct
    22861 tgccttcgcc ttcgatcaca ggccactggc cttcttcatt tggaatgccg aagaatttga
    22921 tatcgctgat atcaataatg acatgctctt tgtaaattcc gtacggcact tcgatggtgt
    22981 caccgggcag cgcctgatct acaacagatt ggatgctttg acctttttcg actctcaaag
    23041 tcaccgcttc atgcgtagac gttgaaactg attctgttcc cccggcattc atctggctga
    23101 tggtttcgcg cgcggggttt tccacatgcg ccaccactgg cagacccgat ggcacagatt
    23161 cggggaattc gggcaggttc gtttcatcgg tgagagcgta catgaacgca acgaggtcgg
    23221 atttttcctg cttggtgagg tcaaagccca aaacgtgaat atccacattt tcgacaccat
    23281 catcgcgacc gccgcctttg gcgtaaaact ccacgacttc ttcgagcgtg gcaaaggcgc
    23341 cgttgtgcat gtatggcgcc gacaaggcaa tgttgcgcag cgaaggcgct ttgaaggcgc
    23401 catccaaact ttcactttcc acttcggcgc ggcctgcatc atgctcttgt ccgggcaggt
    23461 cgggtacgcc tgttaccgag aacgagtcgt cgctgaaggt gggcgcggcg tggcattcaa
    23521 aacagcgtgt gcccgccgaa cggaaggtcg ccagtccgcg gcgttgggcg ggggtgaggg
    23581 cattcacatc gcccgcggca taacggtcaa agggggagtt gtttgtaatg agtgtgcgct
    23641 cgaacgcggc gatggcattt tgcacattct cgtacgtaac ggcatctttg cttccgaaag
    23701 cctgttcgaa tagcgaaacg taggcaggaa tcgctttcag ttgagattct atttcttcgg
    23761 gcgaaccagc catctcgtcc gcgtttgtga tcggggtgtg catctgctct tcgagggtgg
    23821 cggctcttcc atcccaaaag aaattggtgt tgtaggccac attccacaaa ctcattgcat
    23881 tgcgcggaag ggtcttgccg ttcgcgccga gtgcctgctg catgccatca ccgaaaccga
    23941 gatcggggtt gtggcaggtg gcacaggaca tgtcgtcatt ttttgaaagg atcgggtcga
    24001 agaaaagcag gcggccaagc tccgcttttt cgggtgtggt ggcatttgca tccgtttcat
    24061 tggtggcagg gaaatcgcgt tgaagcccgc tgtaggcggg ttccacgctg ctggagcccg
    24121 cgccccactt ttcgggaggc agcacttcat cctgcggcag cggcgcgaat gcggcataca
    24181 aaataccgat gagcagcaaa atgcccaacc ctgaaaaaat acgtttcatc caaactttca
    24241 tacattctcc tcttttggct ttttatttca gtgtcaataa aaatgcgacc agcgcattca
    24301 tatcttcgtt ggtgagttcc ttggcaaagt tggtgatcat cacgttgttg aaaccttcca
    24361 ccacatagtc accaggccga agaatggata gctggatata ttcctcggcg ttgacccctt
    24421 ccacgcgggt ttcggcacgg gtggcaattc cgttcagcga cggcccgatg atgatggtat
    24481 cagggacaag ggcgtggcag gtggcgcatt tggcgttgaa gacctgctgt ccctgcaata
    24541 caagcgggtc tggggtgggg gtgggctcgg cttttgccat ggctgaacaa gctgatagcg
    24601 caagcgcgat caaaagcaga atagtctttt tcatatttgt cctgaattga gatttgaatt
    24661 gcccgattat acatgcatct ttcctctttt ctctttcctc atctttgcta taatcaactc
    24721 aacattaaaa aggagagtga actatgcaaa gtttttcacg aggctgggcc tttctcaaac
    24781 aggcctggga gatggcgttc aaagataagg acctgctcaa accttcgatc tatgcgctta
    24841 ttgtgggcat gatcgtttcg gtcattggga tcattcccat tgttggggcg gccattctct
    24901 ttggcgatac ccagatcgga aacatcattt tgttcgtgtt gggcggcatt ttggtctttg
    24961 ttcaatttgt ggtcacttat gtcttttcag gcatgaccgt gcatttgatc tatggttacc
    25021 tcaccgaagg tgatggcgac atgggcaagg cctgggcgat cgtccgtcgt gatttcttcg
    25081 atattctcac ccttgcagcc gcatccacgg ctgtgaatct gttgcgcagc ctggccaatc
    25141 gcaaccgcag gggcggcgtg gccgccagtg tggcacgcgc agccacaggc ctgctcgaaa
    25201 ctttgtggac ggaagcagct ttgttgatct tgcctgccat gatcattgac gatctcaatc
    25261 tcaaagacgg gatgcaacgc attttgaaga tcaccaagga aaaccttctg ctggtgggta
    25321 tcagtacggt gggtgtgcgt tgggtgactg gcttgatcgg ctttgtgttt ggtgccattg
    25381 gtcttgtcat tgcttttatc atcggcggcg gtgcggcatt cgtttccggt ggcagcacgg
    25441 caatctccat catcggcatt gtcatcggtg cgttgatctt ctttaccttt gtgatgctgg
    25501 ccaacctctt tagctcctac accaataccg cttatcacac ctgcctgtac atctgggcgc
    25561 gccaggttga aaccgcccgt gcgcacggca gcgctgaaca agtccgcgct cctgctccgt
    25621 tggctgcggc gctggcttaa tttattttct gtcctctttt ttgtaatgga ggaaagagga
    25681 cagatgaaag aagaaagaag aattcgatcc catgcaacct gaaccccgcc gagaaatggt
    25741 cacctggcag gagattgacc gcctcattga ccacctgctg cctcagttca aacgtgaatt
    25801 taccgcgatg gtgatgatca cccgtggtgg aatcatcccc ggcggcctgc ttgccgatgc
    25861 gatggatatt actcacatcc tcactgccgc tgtggatttc cccgcgcaat ccaaaacaga
    25921 gaaatccaac ctgatggtgt ggccggagtt cattcagttt cctgctgaag ataaactgcg
    25981 cggacgcccc accctgatcg tggatgatgt ttggggctcc ggacgaacca tcacagcggt
    26041 gaagaatcgt gtgtctgcgg cgggtgggtt tgctgaaacc tgtgtgctgc atttcaatcc
    26101 ataccgcaac ctgtttggca atgcccgccc cgattattac gcggcagtca ctgatgctta
    26161 cattgtctat ccatgggaag tggatcgcgg cgccgacaaa gtgctgcttg gcgagctttg
    26221 atgccaccat gaaaacgatc gaagtaagtc acgaaaaccg tataaaaacc ttgaagggaa
    26281 atgaatactt cgacaacctt cctgaaccca tgttgaagga catttctgag catatgcatt
    26341 tgcgagaata ccagcgcggc gatgtgcttt tttgggaagg tgacccgtgt gatggtttgt
    26401 acattgtgga gcaggggagc gcgaagatct tccgcctttc gccgcaggga cgtcaataca
    26461 ttgtccgcat tttgcaggag ggcgatactt ttgcagaagt gcctgctttt gatggcgcgg
    26521 caaaccctgt aaatgtagag gcgctggaag cctgccgtgt gtgggtgatc gatggcgaat
    26581 tgctgcgcag gctcgtggaa ttgcatccgc attttgcgca gaaggtgctg gcaaactttg
    26641 ggcgaatgct gcgcgggatg gtgcgcatgg tcagcgagat ggctttctat caggtgactc
    26701 atcgtctggc gcgtttgatc gcggaaatgc cccaggagag gtccgcgccg cattggaccc
    26761 aggaacagct tgcggcgagg ctgggaaccg tgcgcgaggt tgtagcgcgt tccatgaggg
    26821 aacttgaacg cagcggcgcg atcaaggtgg aagacaggcg catccagatc gtcgatcaaa
    26881 acattttcag tcaatggatg gaataaagtg atttgaatca aaagcggcct ttgcgggccg
    26941 ctttttttat ttgcattcaa aaatggactg ttatttcaag acttcgaaat tttcaacact
    27001 gatgtataac tcgtcgtcca ccgccgcctc gatctggtca atcgcaatgg aagtgggata
    27061 cccgttgacg gtgtcgtata tcacttcgac ccgatctgca tcttcaaatg cgcctttcaa
    27121 ttgctcgaac agggcttcca tgcctgcata gctttttacc acgtcataca agccatcaga
    27181 cggagtgatg atgtttccct tgaccgaagt aatggaaacg atctcgccgt tgttgatttc
    27241 aatggtcatg ggcatgtcat ctctgaatgc gcagaaacaa ccgatggcga cgtcgtagcg
    27301 ataatgcgtg atctttgcgg cttcccattt ggcgaggttt ttgtcaaact cactggcagc
    27361 ccctgcagag caggctgtga ggatgagtgc cagcaacaat aaaataagtt tcttcatttt
    27421 ttctccttgc atctgtgacg atattttcaa cgcagatgtt cccagaaaaa aagttccccg
    27481 ccattgctga cggggaactg agttgatcgg atctgattta tggggcgggt gttgcgtagt
    27541 aagtgcctgt tgccaacaga ctggtgtcgt tgatggactg ggcggcgagc atttgggcct
    27601 ggcgggcacg gttggtcgct tcggcaagga ctttcaacgc gtattcggga ttgtggaagc
    27661 ccgtgctgtt ttcagccgag acaatatccc acatgtattg ggcttcacgg tgcagtttgc
    27721 gggcttgatc cagcagggca gggtcggcat tgccagccgc aaccgcggcc ttgatggcat
    27781 tgactgcatc aagaatggcg tcttcagtgg tgatctttgt cgtggcaacc tgttcctgaa
    27841 tggtatttac acgattgacg acgaattccg tatctgtgtg gcactgaccg caggcttctt
    27901 ccgcattgag caaggggctg tggacatcat gcgaagagta ttttgcggcg ccatcgcggg
    27961 tgtacggcat atggcagtcg gcacaggaaa cgccagcctt gtagtgggtg ctatcagcgg
    28021 tgaagaattc gtattcagga tgctgggctt tgagcatggg ggttccggtg tcagggtatt
    28081 cccaatcctt gaaacctgct tcttcgtaat aggccagcat ttcatccact tttgtgccgt
    28141 tgtcccatgg gaaggtgaga tacttgccat cacccttgaa ataatattca acatggcagt
    28201 ttgcgcagac aacggtccgc atttcctgac gggtgaaggt ggtccaatcc ttgccctggc
    28261 gttcaaaggc ttcttccagt gcggggttgg tcacgatcag gcgcatggtc tctgcttcgt
    28321 ggcagttggc acagccaatg gcattgttga tgttcggggt catttcattg aaggacatct
    28381 tgtcataggc ttccataccc atctctgccc acaaaccagg gttatcggtt gatttgcaag
    28441 agtagcatgt ggcaggtgtg tgcttgggat ttgagtcgtc aaggttcaaa cgacccgttg
    28501 cacgtacatc ttccaacgca ttcgcgtgac cacggtcgtc gttgtaatcc ttgctaaatg
    28561 gataaccggc aaacaaaatc acctgacgcg gatcgcgttc cagccacgag aactgggaag
    28621 accctgcata ggttgtgtcg atattgttcg tctgggtctt catgaatgag tcgtattgat
    28681 tggggaaatt cacaccccat ttggccgaat caggttccat ggcggcaatc tcaaccaatg
    28741 gttctgttgc tctctcctgc gcgggctgat ttttcacaaa ggtcagtacg cccacgagtg
    28801 cggcagccaa aacgatcaca agtcctgcaa gaataaggtt ggtgtaattt ttcataaaaa
    28861 ttctccttac ttgattgggt agagtgttga atcctggaag gggatggcgg aagcgccgcg
    28921 gtcaccgtgc gccacactgc ggtgacaatc ccagcaatag cggtcaaagg gttgggcatt
    28981 cattacaaca acttccacgg tatcttcgtg gcaatgaacg caattcgcct gaataatgcc
    29041 tttggtcttt tcgctggcgc gaatttgcac agggatgttc ccggttacaa aggcaaacac
    29101 atcctttgca ccctgtctgc ccttctcggc atagtaggtc accacattat cgtggggcag
    29161 gtggcaatcg gtgcatttgg cgaaattctc atgtgcgccg tgataccagt tttcatactg
    29221 tgcatccatc acatggcaat ttgcacaggt ttcgggggcg ctccccccgt aagaggcggc
    29281 atcagtcaca aacgcaaaat acccgaacgc aaggaccgtg gccgcaatgg caataaaaaa
    29341 tggggttttt gacatagcct atcctcagaa aagattaacg tgcgcttaat ataaactggc
    29401 ttctgaaatt tgtttgtaac ttatgtcaca taaagaaaga tgaatttgcc tgctttactt
    29461 agataaattt actcaaaata gtcagattta cccagtgata tacatcccta ttctgacgtg
    29521 acacccgcct cttcctttaa ctggaaactg tttctatata agaagccttt ttctccccga
    29581 aagataaaaa atacgccccg ctgctgatag cggggcgtaa aaggagaatg caccggcgat
    29641 ggtgcgaatt cacaaactat ggcatgtgac catccatacc agagccgccc atgtcctggg
    29701 cttccatcac tggaatggtc agggtgatat cttcgtggtt cttgaaatgc aaggtcagcg
    29761 tgatctcatc accaacatta agatctttgg ttagaccgat gagcataata tggtagctgc
    29821 cgggtttgag ttctaattca ccctctgcgg gcaggggaat cgattcctgt tgaatcattt
    29881 ccatggtgcc gtcggatttc atctggctca ggtggatttc aacagaagct gccgcatcag
    29941 aagaagctcc gaccaattca tcatcggctt cggtgtggtt gtgcaggagc atgtaagcgg
    30001 cgctgtttcc atctttcaaa gctgaacgca cccaaaaatc gtgcgcctcg atgtctgttc
    30061 catccgcatg ctcatcgccc attgtagaac cacaggcgct caagagcagc gtccccacca
    30121 aaaccaacat catcaaaatt cgtttcatga tttatctctt cctttttagt ttgatttgcc
    30181 tgaatcacgc gttctattgg tgcgcgtgca tcattccatg aataaattga atggcatcct
    30241 tcatggttgc aagggtgccg ccaaagccct tttgaaattt ttctgcatct tcctgtgagc
    30301 cgaagctcaa cacactcggt acgcaacaga cctttagatc gctgccaaga acatacacag
    30361 cctgggtggt gctgatgata tgtccgtgca ggtaatcggc ggtgagagat tgagcggcgt
    30421 cctgggtctg gctttgaacc atcagcccgc aatgggcaca gcaggcacgt ttttgttccc
    30481 cgctggtggt acgtacgatg aagaccgtcc gctctgagat ggttttgttg cacatgatgc
    30541 attgattgat acccgcagtg ggtgattggg tgaccagtgc aacgccccca tgtttttttt
    30601 gaatcaaacc tgcttctgcc agtttgttca attcacggtg aatggtcatg ttggaaacat
    30661 tgaagacctc cactagttcc tgtatggagg cacttccctg tttttgaagg tgttccagaa
    30721 tctttttttc gcggtcaatg gtcgatgtca tgtttgttat attatgttat tttgaacaag
    30781 aattcgcaga atataacaaa atgggtgaag actgtcaagt gagatttgac actttcacag
    30841 tgttgattgt gtttttggaa gctgggcagg tgacaggtcg gtttgatgcg ttcctgtcct
    30901 tattccaaaa tgacagcggg ttgtggctgt ttacggaata cgatccaata aaaaacgatc
    30961 attccgatcg tggtggagat gagaccataa acagggtcaa gggtttggac cgtgaacaag
    31021 gctgggctgg gcagggcagt gacggtgtag ttggcaaaca gggcaccgaa gaggttgttg
    31081 gctgcgtgca ggccaagggc aagttccatg ccgttgtctt gcagggtgat gaggcaggcg
    31141 aagaagccga atgcaaaata attcaatccc atcaggattc cgttgacggc catttcgggg
    31201 ttgaccatgt gcggaatgaa aaacagaacg ccgttgatga atgccagcag ccatttgttt
    31261 ttgagcttca atccgatcca ttgcaaaaga taaccgcgaa agaaaaactc ttcggctgaa
    31321 gtttggatgg gaatgagaat gagcgccagc agggtgtaaa ccagcagaag caggggctgt
    31381 aagctgaaga cgtatcgccc ggggtaaagc agggcttcgg tgatggaaat gagtgcggcc
    31441 acaccaaacc atgcggcggc gcccgtcagg attctgtgcc agcggacgtt tgtttcggaa
    31501 gtgatcagtg ttcttaatgg tctggcatgg atgacacgaa tggaaaagat ggttgctgcg
    31561 atgaatggga aaaatgtcag catggtggcg gagaaatcca gcaggacaga gatgcctgtg
    31621 aagccgcttt gtgtgaagtt tgtggcgggg ttgccatcca tcatgacata gcccaaaagt
    31681 aaaatgatgg ggatggagcc gatcacgaac caaatggtga ggatggtgaa aaaggagatc
    31741 aaatatctcc accaatcgtt tttgccagcc tgtgccgctg ccaggtatgg attcataaaa
    31801 gctacctcca ttcgttttgt ggtgaattgg aaattctact cgaagcgggc tgggttttag
    31861 aaaatggacc aggttacaca tgatctgtgc aaaaaatgaa tcatgactac cagaagagat
    31921 tagacctctt tcaaaagtct gaaagattaa ccactgagtc acggagacac agagaaaaca
    31981 attaaaaact ccgtccgcag gaggctccgc actgcctcag tatcttcgtg gttcaaaatg
    32041 gcttttcaaa gacatacttt gcaacgtata cgagaggtca attataaaaa atcccgcggg
    32101 tgcgggattt ttttgatcac tatcaggcgg gggtggcgtt ttcttgtttg agttcaggct
    32161 tgtgttttgc ccaaaagggt tgaatgaaat ttcctttttt cattgcgagt gtagtcagtg
    32221 caatgcctgc cagcagacag atcattacgg caatgatgga ggcttctgca ccgaacgcgc
    32281 caccggagag gagggttggt ccgctcaatg tggattgcaa caggccttgt acttcatttc
    32341 ctgaaacggc tacaccgaag atggcaccct gcgaaaaatt ccatgcaaag tggatgccga
    32401 tgggcagcca caaacggcgg gtaaagacat aggctgccgc cagcatgata ccagcctcaa
    32461 tggcaatggc gatcgcaccc cacaaagtgg cattgggatt ggctaaatgt aaaaacccaa
    32521 agatgacggc ggagattgca agcgcgatcc aggttccgag tgattcttcc atgatgcgaa
    32581 agagcaggcc gcgaatcagt atttcttcga tcacaccaga ggagatcgaa agcaccaatc
    32641 cggggatgag cactgtccat gcattggtgc ctgatacttt ataaaagcca aacagccata
    32701 aaatgccgat ggttgctgaa aagagcaatg cgcccagtgc ggctccgcct gtcaattcac
    32761 gcacggcttg atggggggag agttctgtta cagcgcgttt ttcaattacg cggacaaatc
    32821 cataatatgc aagacttgca atggtgacaa tgatcaatac cgatgcgatc tgtgccagat
    32881 tgttcacatg gccgggccat acttctgtgc caatttgcgc gatggaggtg accagcccga
    32941 taagaaagaa tccaaggaag aagcgggtaa ccgggaattg caggaacttc atgaattttc
    33001 cagaggcgga tgagttttgt ttcatttccc tactcactga ccaatagcca tttggcaagg
    33061 cgcccatgca attgaccaac tccgcggatg atgttcatga aggttgtcca cagcaggagt
    33121 ccgcccagta ccaatagcgg ataggaccag cttggaaagt aatattgaac gccgttgtta
    33181 taaacagcac cttgatgcag aacttcctgt atgaacggga gtgcgatgaa ggaaagtgag
    33241 atgcttaaca ccatgacaat tatgatgaag tatgcagttc caagaacaaa ttggacgatc
    33301 atgtaaacca gcatgagcca ggtggctttg tcggtgagga gggctttcag gcggtcgaac
    33361 cacttcatac cctgatgcga gaatagcgga cgacgcggca tacggatgcc aagcagggct
    33421 tccacaaggc ggccttcgag caaggccagc cctcgcactg agagcagaaa cagcagcgca
    33481 atcggaaacc cgaagataaa gatcaaaaag gaaatggata ctgataatcc ggtgactgcc
    33541 cagctaaagt aaaaaacacc ggtcacaaaa gcgatcaaca tgtaaagcaa ggcgccccag
    33601 gcacgcgggt cggtgtacac accaaaaaat cggcccagtg ctgaacgggg tttggtggtt
    33661 tgttttagtg caggggaggt gcgcctttcc acctctcgat aggcagacgc ggtctcttca
    33721 ggagtaccgt attcttcgat gatcttcccg agggcatccg cttcactgac tgcagggttg
    33781 gcttcacgtg ctgcttcaag tgcggtactc aggtgttcac gtgcatcagc ctgtgcgtcc
    33841 tgcacgaggg cagggtcggc atctttcatt tcggttttca aggcatcaag atattcttcc
    33901 atcgttttaa acatgacgtc atcctttcaa attcgcgtcg acgaattttt tcgtgatctt
    33961 ccagatttca gcccattctt tgagggcttc ccgtccatca ttggtgatct tgtaatacct
    34021 gcgtggcggg cctgaaaccg agggttccac ggtgctttcc aacaaaccgt tctcttcaag
    34081 ggagcgcaac acagggtaca aagtgccctg tttcatcatg gggatctcag ggccgctttc
    34141 ctcgagcatt tttgcgatct ggtatccata catgggttct ttgctgcgac tcaaaacact
    34201 gagcaggaca agcgacgaag tgcctgcatt cagttctttt tgaaacttcc tggtatgcga
    34261 cgaaatttca gtcatttgtg ctcctttcag tagtatcaga tttttactac taataaatag
    34321 ataatagtat gagattgata ttacgtcaag ggctttttat agttccgtgg taccgtttta
    34381 aataatcaat cccgcttttg gcgggattga tgcctgacca ttatttacct gttcccatac
    34441 tcaagccttg gcagtgattg caaataggcc cagaccgctt tcaactcatc atcgctcatg
    34501 aatttgtatg aattccacgg catgtaggcg gcgcgcaatt ccttgccttg cggtgttttg
    34561 ccggtgcgaa tggtgttcat gaactcttcc tccgtccatg tgggcagggc ggaaccgccg
    34621 ccaaaagtga tgtttggggc gggcggccag gtggatggga agccgggaat cacgccgccc
    34681 gacatgttta gtccatggca gacgtggcag gactgcgtca ggtaggcccc gtattcaggt
    34741 gttgtacccg ctttcggcgc ggtcggacgc gctgagtcgt gtgggatcag ttccgcgggg
    34801 ataaaggtaa tgtctttcac cagagtcatc acgatccgcc cggtgaaaga gatttcgctg
    34861 gcgggcaggt cattatcaac tggtgcaacg ctcttgatat aggcgatcac gtccccaaga
    34921 tcatcatcgc tcaaataata aaattcagtg gatggcatga agagataggc ggtgccattt
    34981 gggcggatcc cgtgacgaat tgcgcgcacc cagtcagaat cagacattgc gcttcccacc
    35041 ccgcccaagc ctgatgtgag attcgaagag atgactttcc ccacggcagg atcatccaaa
    35101 taaaccgtgc cctccaggtt ttcgccgtga caggcttcgc atccccgaaa ttgaaaaatg
    35161 tgttttccat tttcaatgga agccgcgtcg gttggaatga caacttgttc tgctggaatt
    35221 tcatacacgt gtgtcagcct tgattcgctt tgcacataaa tgataaccaa cccgatggca
    35281 accagccctg ccaatatccc caacacaatt cccacccact tcaaaaactt tttcattctg
    35341 ctctcctctt tacaaattta caaaataagt cccgcctcgc agcgggactt ttgtttttct
    35401 cttttgtgtc tttcgtttta cggttgttga atttccgctg cgcgcaatgt gttgttcatc
    35461 aacatggcaa tggtcattgg gccaacaccg cccggcaccg gggtgatgaa gcctgccact
    35521 tctttggctt cttcgtaatt cacatcgccc acgagtttct ggcgcggttt gccggtcttc
    35581 tggctgatga cgggggtgcc atcggggttg agcatgggaa tgccattgat gcccacatca
    35641 atgacggctg caccgggttt caaccagtca ccacggacca tttctgcacg gccgatcgcc
    35701 gcaatgacga tatctgcctg gcgcaacaca cccggaatat cttttgtgcg cgagtgaacg
    35761 accgttactg tggcatcttc ttttacaagc agcaacgctg caggcatacc gacgatgttt
    35821 gaacgaccca atactacagc attggcgccc ttgatggtta cgcctgcttc tttcaaaaga
    35881 taaatacagc cataaggtgt gcagggaaca aaaagcggtt cgcgtccctt ttgggcaagg
    35941 cggccaagat tgatcggcga gaagccatct acatcctttt cgatgctgat gagctgcaga
    36001 acgcgctctt catcaaggtg agagggcaga ggcagttgca ccaaaatccc gttgaccttc
    36061 gggtcggcgt ttaattcctt gatcagtttc tcaaggtctt cctgtgatac atcggcaggc
    36121 agtgggtgat gataggaagt catgccaagt tcagcgcagg ccttctgttt catgcccacg
    36181 taggcggcag agtcaacgcg gtcaccgacc aatacagtgg ccagtccagg ttgtgacttt
    36241 cccgccgcga tccttgcagc gactttctct ttaacttcat cacgaacctt ctgcgcgatg
    36301 gcggtgccat caatgatttg agctgtcatt ttttgctcct ttgcaaattt actgtgaaag
    36361 attgcacgag tattatacat tttctccccc ttttcaaaaa gtgacactta tcccgatttc
    36421 ggttcttcaa acctcaagga aagtttttct tgaggtttgg gaggcaaact tcgtccagac
    36481 cgtttttatg gtttcaatgc cacaaaatta tcgaggatca catccacacc gggtttggca
    36541 aaagtgccca ccaacatgcc tacatcacct gattttaaag ttgcatcctg cacagcggcg
    36601 atctggaatc cattgacata aaaagtgagt tggtcgcctg tacaatcggc gcgcaaatgg
    36661 ttgaccgcca gccctttgtt gatgttttcg ctcgtcagca tctcattttg gccgagcaaa
    36721 acagcctgtc cgcccgaaaa aaggccgact ccataatatc catcgctggt gatgatgaag
    36781 aagtagtaat ccgcaccgga agagcggcag atcaagccga tgcggttttc atcgggaccg
    36841 cccaatttgc cagcgtccac ttcaaggcgg acatcggtaa agttcttttg tggtgtcgac
    36901 cagaagttcg tatccaacgc attgaccaat atgcggaaac taccgctgtc ataatccatc
    36961 acaccttcgg cggcagaaaa acgactccaa cctgagccta ggttggcaaa atcatcttga
    37021 aagaggactg ttccagatgg cacggcctgg tcctgcgttg cagtgccgga tggtgcagct
    37081 acagtgcagg ctgaagtaaa gagaatgacg agggaaaagc aagcgaatag acgattcatg
    37141 caggcgattt tatcatgcca agatttatgc agggtgggaa gatgagcttg gtttgacctg
    37201 taagaatgaa ctcacccgcg agaacgagct ttcgcgggtg agacagagtt acttatttgg
    37261 atgtttttga ctttgtcgtg cttttctttt tcgttgtaga agttgatgtg gttgctgcag
    37321 ccgtttttct cttttttaca ggagcagggg tttcttccat ttcctcatct tcgtcttgca
    37381 tttcaatttc tgtttcttcg tcactatctt ttgcagtgga tgcaacagaa gttttgcgtt
    37441 tgggggcggg ggcggcctca accttcttaa gttgatattc ctcttcccag ttggcggcaa
    37501 aagccagttg caagcggcca taacgcacca tcaacatgac tgcacctgca accgccatta
    37561 acaatgaaac caacatggtg aatgtgacaa atgaatcccc gatgtgcggc tggtcagggc
    37621 ggaagaattc gataaatgcc cgagccaaac cggcaagcac aagccagccc gcaaagatcg
    37681 cacctggttt caacttttct tcgtattgac gtgccaccca aagcaataac aatgccagaa
    37741 gaatattcag aatcatctcg taggcaaacg tcgggtggaa gcgggttgtc tcaacaggga
    37801 actggctgag gtcagcatat tgagcaaggc gatgagaagc atcgatcgaa atgccccacg
    37861 gcagctttgt cggctggccg tataactctt gattgataaa gttggcaggc cgggccaaag
    37921 cctgaccgat gaatacggcc ggtgcaattg catccaaaaa gagccagaaa tccaatccat
    37981 tggctttcaa aaagaaatac aaggccaatg caccaaagag caggccgcca aagaaatgca
    38041 ggccgccttc ccatacttgc aaaatctttc ccggattatc tgtgtaatac gaacttccgc
    38101 cgagaatgct gttgagtacg taccacaaac gcgcaccaat gatgcccgca ggtaataccc
    38161 aaaccattgc atcccagatc acttcaccgt tctggccgcg cctggtgatt tccttctctg
    38221 ccagccatgc accgaccaca gccccaagca taaccaacac accataccag cgcagaacaa
    38281 tttctccgcc aaacaatttg aattgaaaaa ttacaggatc catttctttc tactacctca
    38341 cgatttattt ttgaaaagat tatatcattc aaagttggta gggattttct caacgcagtt
    38401 gcgataattc agataaaaaa tgtgtaataa atactgtaca gaccgccaca aaagatggga
    38461 tgattgtcat gtgccgatgt gattaatggc aggtcaaaaa aaactaagaa attcttacaa
    38521 tagccctaaa cattgactct tgcactcaat attgaactgg aaacgttatg ctccgaatca
    38581 accgccaaac cgattacgca gttcgagtaa tacttgccct ctccaaaaag gaagagggca
    38641 agcgcgtttc cacttcagag gttgggcgtg aaatgcttat tccccccgcc ttgcttcaac
    38701 gcattgtggc agaactggca agcggcggtt ttattcttac ccaggccggg cgcgatggcg
    38761 gcattactct ggctcattct cccaaacaga tcaccctgct tcaaattgtc gagcattttg
    38821 aaggagaact ttatctttcc gattgtgttc tcaaaaccgg tgattgcccg ttcgaactgc
    38881 gctgccctgt tcactgtcaa tgggtgaggc taaagaatat gctgcggaat gaaatggcac
    38941 agacgacctt ccaacaattg gtggatgaag gccaacaaat cgaagccaat ctgataaaga
    39001 acgcttcatc cgcaccgata cctgattctg ctccgatcaa aatccttctc cccgaggaaa
    39061 tccctgcatg aacaagtatt tcctcggcag tttttatggc ttgtttggct tgattgttgt
    39121 ttctgctttc gcttttaagg tctttcagcc gattaaagtt ctgccgcgta tgcagctttc
    39181 acctgctttc agcctcacag accaagacag taaccgtctc accagcgaag acctgcgtgg
    39241 acagttcgta ctttacacat tcgaatacac caattgtcca gaggtttgta acagcatcaa
    39301 cgacaccatg cttgaagtgc aatcccgcct cggcgaagtg gaattggacg gtatttccgt
    39361 agccttcgtc accatctcca tggacccgga gcgtgacacc cctgacgcgc tgaatgccta
    39421 cgcccaatcc attggcgcag atacaaatcg atggaaattt gcaaccacaa ccaacgaagc
    39481 aatgctcaag accatcattg gttcgggctt tgaaacctat tacgaaaaaa aggaagatgg
    39541 tagttttgcc tttgacccat cctttgtgct ggtggatggc tggggcattg tccgcgcaga
    39601 gtatcgctac gccaccgaag tttccaatgc agaccgcatc ctccgtcacc ttggcgtgtt
    39661 agcagaagag gttcgcaaca gcaagggaac caccaagctg gcttatgaag cggctcattt
    39721 attcctctgc tatgctccat aaaatatttt gaattatgaa aacaaagata ctgctcggta
    39781 cattcgccat tattttaggc ctggtaacag gagtttattt tttccgtccg catacttttc
    39841 atggcacggt gatccagtct cctgaaacat cctatgattt caacctgacg ggcggcaaag
    39901 gcgatgtttc gctaagtgat ttccgcggca agttggttct gatttatttt ggctacacct
    39961 tctgccccga tgtttgcccc gccacactgg gacaagtcaa tcaggccatg aaaaaacttg
    40021 gcagcaaggc agaagacatt cagttgatta tgatctcgct ggaccccgag cgcgatactc
    40081 ccgcaaaatt ggaagaatat gtcgctcatt tcaaccccac ctttttaggt attaccggta
    40141 cacaagagca ggtggatact gtcgcctctc tgtatggaat tttctatgag aagaaggaag
    40201 gcagtgcggc aagtggttac ttgatcgatc acaccgccac tctcatggtg atagaccgtg
    40261 agggatattt gaaattggta tttccattcg gcgtgactgc tgatgagatc gccgatgatt
    40321 tgaagtacat gctaaagcaa tagggaggtg gaactgcaac agcgtaagat caaccccatt
    40381 tccacttccg cagtttgcgg cgccgcatga agcggtagtt atcctaatcc atcattttta
    40441 agaggagaat gaaacatggc gaaaaaagat aaagaagacg atgagtttcg cccgactggc
    40501 acgatcgcag ttttggcgat ctttgtagtc acactcatcg caatctgggc aacgatctac
    40561 atgatccttg tcggcagagg aggcacatta tgagcaccaa ccagattcac attgattcct
    40621 atgagcgtaa ctggatgatc gtcagcgcca tcctgctcgt tttgtttgca gcggctgtca
    40681 gttttgctgc cttcggtatg ggaattcagg tgcctgcccc ggaacagcgt gttgatcccc
    40741 gcactgtggc aaccgacccc aacagcccct ggtcgaaccc cggtttgcgt gaaatcgaac
    40801 ccggcaagta tgatgcctac attttggctc gtgcgatccc cagctgggaa tatctgccca
    40861 aggaactgac cgtacccgca ggctccagtg tatccttcta tgtaacttca tctgatgttc
    40921 agcatggctt caagattcag gatacaaacg caaatttcat ggttattcct ggccaggtat
    40981 caaaactgac catcacattt gacaagcctg gtacctataa cttcatttgt accgaatact
    41041 gtggcagcgg tcacggtgtg atgtatggcg cgatcattgt tacagaatag ctgtgaagag
    41101 agacatcatg aaacaatatc aattatctgc tggtgaaaag aaattcattg gctcacacct
    41161 tttagtggcc attttggcgc ttgctgtggg cagtttattt ggccctctgc aggcttttga
    41221 acatgcgggc tgggatttct atgtttatct caagcctttc attaagtcct actatcaggg
    41281 acttaccgtc cacggcgttt tgaacgccct ggtttggacg accttcttca tcatgggctt
    41341 tctcactttc aatgtgattt ttggcctgaa acgccctctc aaaaatatgg ccctgaacaa
    41401 ggttggtttt tggatgatgg tggtcggcgt tgtcgctgct gcgattccga ttttggccaa
    41461 tcaagccact gtgctcttta ccttctatcc tcctctcaaa gccaactggg ctttctatgt
    41521 agggttgacc cttgttgtgg tcggcagttg ggtggaaggc tggggtttgt ttatgacttt
    41581 tgccgcatgg cgcaaggaaa accccgatga acgttcacct ttcattgctt ttgccagtgt
    41641 tgtaacagcc ctgctttggc agatttgtac cctcggcgtt gctgtggaaa tcctcaccat
    41701 gcttctgccg tggtcacttg gcttgattga aggcgtagac ccgcaattgg cccgcaccta
    41761 cttctggttc accggccatc cgttggtgta cttctggctt ctgcctgctt acatctcctg
    41821 gtatgccatg cttccgaaac aggctggcaa tggcaagatg ttcagcgatt cacttgcccg
    41881 ccttgccttc tggatcttcc tcgtcctctc cacccctatc ggtattcacc atcaatatgt
    41941 agacccgggc gtttctcctg tttggaaatt cctgcactcc atgtttacat ttggcgtatt
    42001 tttcccatcc atgatgaccg ccttcactgt ggtagcttcc cttgaacatg ccgggcgcaa
    42061 acaaggtggc ttaggaatgt ttggctggat tggaaaactg aactggagcg acccatccgt
    42121 agctacacag cttttggctg gcatcctgtt cttctttggc ggcatcggtg gtttgacaaa
    42181 tgcctcctac aatgtcaacc ttgtgttgca caacactgct tttatccccg gtcacttcca
    42241 cctgacggtg gctactgctg taaccctcag cttcatgggc atttcctact ggcttctgcc
    42301 atatctcact ggcagaaagt tatggaaccc caaatgggca gttgctcagg catggacatg
    42361 gtttgttggt atgatcatct tctccaactc catgcatgtg ttgggtttgc tcggcgctcc
    42421 ccgccgcgta ccacttggtt tggctcccta cgttcctgct gaatggaaca gccgcctctt
    42481 ccaggtgggt gtgggtgggg ccatcctctt tgtcagcggt gcaatctatc tcacggttat
    42541 gctcatgact gccttaaaca aggaaaaggt taacgaagag gtgattgtcc ccattgctga
    42601 atccatgcaa gaggtgtctc gcaccccggt gtggcttgac cgctgggctc cgtggattta
    42661 tggtgcgctc ggactgatcg tcattatgta cggccctgtc ctctttgaaa tgatcaagaa
    42721 catcgccctg acctccccag gcttcagagt ttggtaattt gaaaacgaaa gtgacagtca
    42781 cctttcccgc aaggtgactg tcactttctt cttgtctgtt tcatttcgca aactttattg
    42841 attgacgtct gcttttatcc actattactt ccgcgaaatt atttgtaaac gaaactgtca
    42901 gcaaaacaca tggctacctc cccgactaaa aaacaactca tcgatgttct caccgagcgc
    42961 aaccccagcg tggattggaa agcacgcctc aattcaccca gccgccgcct gctcaatttt
    43021 ttcgaaagca tttctttgcg ccttgaagca cccgtcaact ggctcatcaa tgacccgcgc
    43081 ttcaatccgc tgtatcatac aggcaccatc accatttttc ttttggtggt tattctgctt
    43141 acgggcatct acctgacgat gttctacccg tttggattca ccttctcgta tcaggcgata
    43201 tccaaaatcg aggcgaacct gatcggacgc atcatccgcg ccatgcatcg ttatgcatct
    43261 gatgctgcgg tgatttttgc cctcctgcac ggctggcgga ccttttttca ggatcgtttc
    43321 cgcggaccgc gctggctggc atgggttact ggtgttggta tggcagtggt ggtttgggcc
    43381 attggcatca caggctactg gctgatctgg gatgaacgtg cccagctcct gaaccaaagt
    43441 cttttcagca tccttggcag tttcaaaaac ggacagacct tcatcgttga tttcattgtc
    43501 ggcgacgcgg caggtacagg ctgggtcttc atgatgatcg tcatcgtgct gcatttaggc
    43561 ctatctgcgc tgaccgcctt cttcctctgg ctgcacctca aacgaatgag ccgcgccaag
    43621 tggctgcctc cacgatattg gatggcgatt tcggtttttg tactcttgct ctcggctgcg
    43681 ctttttcccc ttggtatgtt gccgccgctt aatgccacgc aacttcctgc cgaagccccc
    43741 gttgacttat tctatttatt ctatctttca accttcctgc gcgggcctca agccctcttc
    43801 tggagtgttc tacttttcat tgtcggcctc gttactgctt tgccgtggat cctgccgcgt
    43861 gccaaagaac tcgaacccgt aaaagtggat ttggccaatt gtgatggctg caccctgtgc
    43921 gaacgtgact gtccctacct tgccattaag atggttcccc gtaccgatgg tgccaggcca
    43981 aaatttcagg cggagatcga cccgaatctt tgcgtttcct gcggcgtctg tattggtagc
    44041 tgccccgata atgccctgac ctttggcgat attcccctca acccactttg gaaaacaacc
    44101 ctcgagcgaa tcgctcaaga tgacaagagc aaggtaaaag ttgtcttcac ctgcgagcgc
    44161 catgccctgc acggtgcagg gaagcatttt gaagacccaa atacatacat tgtcccgctg
    44221 acttgtattg ccatggcgaa tcccaatctg gctgcacagg cactcgaagc gggtgcaagc
    44281 gatgttcaat tcatcggttg tccgcccgaa gattgtgcca accgtgaagg caacgtctgg
    44341 ttgaatgatc gtgtctctgg cgaaagactg ccaaaactca aacagaactt tattccgctg
    44401 gttcacactg cctgggcggc gcctgctgat tttggtaagg cgatcaagac tcagattaag
    44461 tctgaaccga atacattcaa gttcacaccg aacgcatcgc atcttcgatt catcatcccc
    44521 ttgcttggcg tgatggccgt tgtcactgcc ttccagatct ggttgagtga ctggccgaca
    44581 aaattcttta acgaagattc tgctgttctg gcgattcaga tgacccatca cagtggatac
    44641 cccgttcagg atgtgacgcc tcttcctccg cttgagcctg atcttgaaca accgattcgt
    44701 atcacgctgg aagtggatca cgaactcgtc tttgacgaaa cctacaccgc caccgataac
    44761 catatcaatc aaggcgctcg tatttttgag catatccacc tgcccgttgg aaggcaccac
    44821 atcaccgtaa aaatgtacga ccgtgccgat gccagttttg agcaaatctt gtttgatgat
    44881 acggttaatc ttgagcctca acaggcgttg accatcaatt tcagagatgt ccacattcca
    44941 gaccccaagg caggggagca gctttactac gaaaccgctt caggggttaa cgcgggctgc
    45001 cgcatttgcc attcgcttga aaaaggtgag accatcatcg gcccgtcgtt ttatggtatt
    45061 gccaaccgcg ccggggagcg tgtacccggg ttgactgccg aagagtacct gcaccaatcc
    45121 attgttgacc cgaatgcgta tgtggtttct ggttatcctt cgggacagat gatccaaaac
    45181 ttcggcacga tcctcactga agaggaaatc caggacctga tcgccttttt gttgactttg
    45241 gatggacaat aaaaaatttg aaatggacaa tgagtgcttt tcattcattg tccattgttt
    45301 tgataagagg aaaaatgaga cgatttagct tgttgatttt gttgatcgca ttgggtcttt
    45361 ctgcatgcgg acgtaataaa ccaggtccgc tgggtgcaga tgcctctgcg gataatattc
    45421 ccgatattgt aggctcctat gccttgaacg ccaccgaccc aaccggcgaa gaatacggcg
    45481 gcaccctgac cattacacaa ggtggacagg ccaatgagta caaatttcaa tggctgatct
    45541 caggcggtat tcaagagggg acaggtaccc tggaaggcaa tcaattaatc ttcacctgga
    45601 acagccttgc aggaactgac cagaccgtgt ctggcacagg tcaatacacg atcacggtgg
    45661 atggtcaact ctatggcact cgcaccattg atggcctgga tacccccggc aaggaaactg
    45721 cataccccaa ccccaaataa acagggtttg gactcaatca taaatttggc tgttaaattt
    45781 atgtgttggc aaaaatcatc acagggaatc aaataaaaca attcgtacca aattaatcgg
    45841 ttttacaccc ctgtggggga caaaaaaatc acccgtcaac gggataaaaa atgaatcaca
    45901 aaaatgctat gatttcatca tggcattttt tgttgacgct tgttaactcc ccgagttgtt
    45961 gcaggcgctg actacactaa aacgcaagga gacatggtat atggcaaaag ttgttgtgat
    46021 tggtgcaggg tttgcaggtc atacagccgc gttgtatttg gggagccgct tgggtaaaaa
    46081 acatgagatc acggtcatca gcaaccgtga tgtgttcgga tacgtaccgt catgggtatg
    46141 ggtgggggtg gggcacatga aacctgagca gaccattttc aaactcaaac ctgtgtacga
    46201 caaaatgaat gtaaagttcg tgcatgccgc tgcaaaagaa attcaccccg atgcaggcga
    46261 ccaatatgta ttgggcgaag gagttgcttc tgggcaggaa gtgcgccttg attacgatta
    46321 tcttgtcatc gcccccgggc cgctgcttaa ttttgcgggc acacccggcc ttgggccaga
    46381 aaatggtttt acacattcca tctgttccct gccgcatgcc attaaaactc gtgatgccta
    46441 tctcgctttg tgtgaccgca tgaagaaggg agaaaaggtc aaactggtga tgggtacggg
    46501 gcatcccggg gcaacctgtc agggtgccgc ctttgaatac attgtcaatg ttcacaagga
    46561 tctggtcaaa aaaggattgc gtgataaagc cgatattctc tggctttcca atgaacccgc
    46621 catcggtgat tttggcgtga atggcgtgca gatcaaacgc aatggacaca tcatgcgcag
    46681 tgaggaattc ctcacctcgg ttttcaatga ccatggcatt cgatatcaag tgcaaactgg
    46741 tgtcaccaaa gtggaggaag gcaagatcca ctggcaaaat tatgatggcg aagaaggcac
    46801 caccgatttt gacttctcaa tgatgattcc gcagttcaaa ggccagccct tcaaatacat
    46861 cgacaaaaac ggcgaagaca tcacctcgaa gataaccaac cccgctggtt ttgtccttgt
    46921 ggatggaaaa tacggtctgc cttaccccga gcttgcccaa acacccgaag catggccctc
    46981 ccaatatcag aatcccattt acaaaaacgt cttcgcggca ggcatcgcct ttgcacctcc
    47041 cgggccaatt tccaaaccgt tcaccaacaa aaacaacctg ctcattgcac ctgcacctcc
    47101 acgcacaggc atggtcagcg gggtgatcgg acgtgtagta gccctcaaca ttgtcgacct
    47161 gatcgaaaaa ggtgaaatga cccatcgcga gccgatgact gaaatggtgg ccgcctgtat
    47221 tgccagcatg ggcgattctc tttgggatgg caatgccgta tccatcgtca tgcagcctgt
    47281 ggtgccagac cgcaaaaaat accaaaacga attcggccgt gacctgttca cctcgcatct
    47341 cgaaatgggt gtcagcggcg catggatgaa attcatgctc caccacacct tcctctggaa
    47401 attcaaagcc cgccccggct ggaccattat ccccgaataa ggagaaggca tatgcagacc
    47461 tcacaggaat tttccaaagc agaaaaattc tggatgaact tcaaacttta tcaactttgg
    47521 cgctttgttg tgctaaatat caaaatcctc atggtggtat accaaagtaa acgttcgtaa
    47581 gattttttcc ttcttccata gcaagccgta tgagagatgt aaaggcatct catcatacgg
    47641 cttgtttata tgacttcaat attattgtgc ttgcaccccc tgcatggaag tgctataatc
    47701 gcggcatctc cccttgatat ttgacaattc atttttgtca aaacccagac gtaatttggt
    47761 cgcgcctgga agccgcaacg ataaccttga gcaaaacccc acacacacca aaaatttcct
    47821 gaggaaattc gccagccctt aaaaagggac tggtgtcttt cacattccaa accatatccc
    47881 ataagagact acaccaacat gaaaatactt gaattagaga atgaaccgct atccaaactg
    47941 cgcggcatgg caaagacgct caatattcca aatgcgaatc gtctgaaaaa agagaccctt
    48001 gccatgatga tccgcgaggc ggaggcgcaa aaagaaggca ttgaactgcg cggcggcatt
    48061 cttgaaataa tgagcgaagg tattggcttt ctacgtgcca ccaattaccg catcagcgat
    48121 caggatgttt acgtttcaca ggcacagttg agaaaatatg accttcgcgc aggcgatctg
    48181 gtgattgggc aggtgcgtcc tccccgcgaa agtgaaaggc attttggtct tctcaaagtt
    48241 gaatcaatca acgggctcga gcctgagcca ggaaactccc gtagagttgt ttttgaaaat
    48301 ctcaccccga tcttccccga taagcgtttt gatcttgaaa ttggacacga ccaactggcc
    48361 ccgcgtctca tcaacctcat tgcaccgatt ggacgtggcc agcgcggatt gatcgtttct
    48421 cctcccaagg cgggcaaaac cacgattttg aagcaaattg caaactcaat ttcaaccaaa
    48481 tatccagacg ttcacttaat cattgccctc attggcgaac gccctgaaga agtaaccgat
    48541 atggatcgtt ccgtggatgc ggaagtggtt gcatccacct ttgacgaacc cgttaccgcc
    48601 cacgttcgta cagcggaact ggctctcgaa cgcgccaaac gtttggtgga aattggccgc
    48661 cacgtggtca ttctcatgga ctcgattacc cgtttggctc gtgcgtataa ccttgttgtg
    48721 aatccctcag gccgtaccct ttctggtggt atggatcctt ctgctttgta tccgcccaag
    48781 cgtttctttg gcgcagcccg taatgtggaa gatggcggct cgctcaccat cattgccacc
    48841 tgtctcgtgg ataccggctc ccgccttgat gatgtggtct atgaagaatt caagggaacg
    48901 ggcaacatgg aattgatcct ttcccgcaag cttcaggaac gccgcatttt ccccgccgtg
    48961 gatatcgaac gttcgtccac gcgccgcgaa gacctgttac ttggcccaga cctgctccag
    49021 cgtgtctggc ttatgcgccg catgttcatt caaatgatca caccgcctcc tggtggggca
    49081 gggatggacc tttccgttgc cacggaagcc atcatcaccc gcatggaacg tgccaagaac
    49141 aacatcgaat tcctcgagaa tctgacaaag gacgcgtaac gcgaactgtc gatttgtact
    49201 acaattgaaa gaatgaaaac cctttttgag aaaattaatg ccttcttcac ttccattggc
    49261 ggcagattta agcgcaagcc aaaggaagtg tcggcggtga atgaacctcc caccacaccg
    49321 cgtggacaag gtgcaaagcc cagggtggat cgttttacga tcatcagttg ggcggtgact
    49381 ttgttgatcg ttgtgtcgct gttgggttcg accctttttt ataagagcac ccttgccaat
    49441 gccgctcccg tagttgtgca gcccaccccg gaagaagctc cagttgctgg tcagcctcag
    49501 gtgggcgtac ccgctgccgg gtcgggtgga ggcggggttt tatccattct tcgacgattg
    49561 caattgaaga cgaatatccc ggaacggcct cgttatgcgt ctaccacgta ccgtgtatcg
    49621 cgtggtgatg caatgctttc gatcgcagac gacttcaaac taaaatctga atccattctt
    49681 tttgccaacg atcaattgga agataaccca cacaacctga aacctggtat ggagttaacc
    49741 attccgccag tggatggggt gtactacgag tggcaggaag gtgacacttt tgaatcggtt
    49801 gctgagaaat tcagcgccaa tgccgatgat attgtgaact tccctggaaa cgacgttgac
    49861 ctcaccgacc ccaagatcga accaggcaca acggtgatgg ttccaggcgg cacgcgcgat
    49921 ctgcgaaact gggcggcaga cctgcaaacc atgggcaggg gagcggggac cggtacaggt
    49981 ggtggcaatg ccaccaatgc ttgtggcggc ggcccggtag ctactggttt tggctggcct
    50041 gccgatgacc acagcctttc tggtaacccg tatgggccaa gccatcttgg cattgatatc
    50101 tctgcacccg agggttcaaa tgtatatgca gcaggggcag gtgtggtgac tcaggctcaa
    50161 ggcggctgga attatggcta tggcaacgta gtgcagatcg accacggtaa cggctatgta
    50221 acggtttatg cccacttaag ctcttacgtt gtcagtgtgt gtcaatttgt ggggcagggt
    50281 gcagtcatcg gctattctgg caataccggt aattcctttg gcgcacattt gcactttgaa
    50341 atccgcatcg gcggccagaa tatcaacccc tacgatattg ttcaataaca atgcagacct
    50401 gacggttttt caaaaaagcc gtcaggtctt ttgattccat gaacgaaaac tccctcaaaa
    50461 aagcactttc caaattaaag cttggcggct tgcgttattt tgattccatc ggttccacca
    50521 atgatgaggc gcttgcctgg gctgccaccg aaggcggggc ggcggatctc tcgctcgttg
    50581 ttgcggatga acagacacag ggccgcggcc gactcaaccg aaaatggttc accccaaagg
    50641 gcagtgcgct tgcctttagt ttgattttgc gctcttcaga aaccctgcgc ccgaatctct
    50701 cgcggaccgt gggactggcg gcgctttcaa ttgcagaatc ctgctctaaa aacggactaa
    50761 ccccatccat caaatggccg aacgatattt tgataaacgg aaaaaagctg gcgggtattt
    50821 tggtggaaac cgtttggtct ggggatgaac ttgacctgct tgtcatcgga gtgggcatca
    50881 atgtcttcaa atcatctgtc cctcctgccg acctgttaca gttccctgcc accagcctgg
    50941 aggatgaact gggcaccccg cccaatcggg aggaagtctt gtccagtatt cttgaggcac
    51001 ttattcattg gcgtgcccga atgggaacgg atgaatttat caaagcatgg gaaaatcaac
    51061 ttgcatttcg aggtcagcaa gtgcagacct ttgcagggac tgatgtaccc gtagtggggg
    51121 aactgcttgg cctggagcct gatggaagcc tgcgcctgct tgataaacat cgcaaatcca
    51181 ttgttgtccg ctttggagat gtcagcctga ggccagacgc atgatatact tttaaaaata
    51241 atgaggtgac catgaatgaa tttgacaatc tccgtgaaga tgccgcatcc agttacgacg
    51301 aggccccagc gccaaaatat ttggaggtga aaggacctgc accaaccttc actgccaagc
    51361 ctcgttcgag gcgtattctt ggaatgacag gccagcagcg tttcatcctt tctgtcatgt
    51421 tcatgcttgc cgtgtgcgtg atcggcttta tggctctttt tatcactggc aagatcggtt
    51481 tctgaaattt cctgaaataa aactgcctga cacaaatggt caggcagttt ttgttttaac
    51541 tatgttgtag gttcgttggg cagttacacc agttttggct gttcttccac tttttcagct
    51601 tcaaggacct gtgcgcctgc tttcttcaaa tcctctgcag agatgcgtgc cacagaggca
    51661 acattgtcac catcctgcgg cttgatgagg tggacaccct tcgtagcgcg gcccgattgc
    51721 ttcacatcct tcactttcaa acgcagggtc acgccattgg aggtcatgat cgtcagatcg
    51781 tccgcttttt gaacgacgcg ggccgcggcg atcttgccaa tctctttcag ggctttgggg
    51841 tcgatcgtgt aatttccgct ggtggcacgt cccttggcgg cgtaatcact gagcggcgtt
    51901 tgcttgccga agcccctggt ggtgacgacc agcaaagcgc cgtctttttc gaccacgtcc
    51961 atgctggtga ctgcatcgtc tttcttgagt ttaattccct gcacacccgc cgcctggcgg
    52021 cccatggcac ggacctttgc ttcgctaaag cgcagcgcct gtccattttc ggtgacgatg
    52081 atgatctcat ccttgccgga agtgaggcgg gcccatccca attggtcgcc ttcttccagt
    52141 gacatggcga tcaggcccga gggacggact gacgcgaatt cttccatgct gacgcgttta
    52201 atttttcctc gagaggtcat caacatgcag aagttgtgcg aagaaaaatc tcccacggcc
    52261 atggctgcgg tgattttttc attgggatca agcgacaaca cgttgaccag tggaatgccc
    52321 ttcgtggcgc ggtctgaatc ggggatttga tacacctttt cagagtagac cttcccctta
    52381 tctgagaaga acagcatggt gttgaggctg cgcgcaggga tgagcatcat cacttcgtct
    52441 tcatccttgg tggtatgccc catcacaccg cgtccgccgc ggctttgtga tttgaaggca
    52501 ttggctgcca cgcgtttaat gtatccgcgt tcggtcaggc tgatgagtac agactcatcg
    52561 ggaaccagat cttcttcgtg aatattctct tgcgcttccg ctgcgatgcg cgtacggcgg
    52621 tcgtcgccat aggtctctgc caactcgttc aaatcagatt tgatgagtgc aaggatcttc
    52681 ttggggtggg cgagcaggtc ttcaaggtag gcaatggttt cgcggacctg cttgtgctct
    52741 tcttcgattt tccagcgttc gagagctgcc agtctgcgta attgcatatc taagatggct
    52801 tgtgcctgca cttcgctgag tttgaagcgt ttgaccagat ttgctttggc ggtgtccaca
    52861 tcctctgctt cacggatgac tttgataatg gcatcaatat tgttgagtgc gatcaggtag
    52921 ccatccaaaa tatgcagacg tgcctgcgcc ttggccaggt caaattttga gcggcgtgta
    52981 atgacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatgc agacgtgcct
    53101 gcgccttggc caggtcaaat tttgagcggc gtgtaatgac agtttggcgg tgctcaataa
    53161 agatctgcag catgcgtttg agcggcaggg tgcgtggttc acctcgcccg ctttcatcgg
    53221 cgaccagcgc cagcatctgc acaccaaatg tggattgcag tgccgtatat ttgtaaagtt
    53281 gattaagtac cttcttgggc tgcgcgccgc gcttcaactc gatgacaatg ctcataccgc
    53341 gctggtcaga ttcatcgcgc aggtctgaga tctggtcaat tttgtcatcg cgcaccaact
    53401 cggcgatgcg ttcaatgagg gtggatttgt tgacctgata cggaatctcg gtaatgttga
    53461 tctggtaacg gccagctttt gtctcttcaa tgtgggcaat gccgcgcatc acgattcgac
    53521 cccggcctgt tccatacgcc gaaaggatcc cgtccgcccc gacgatcatg ccgccggtgg
    53581 ggaagtcggg gccttgtacg aatttcatca aatcgttgac cgttacatca tcaatggtgt
    53641 cgtgatggtc gatcaaataa ttgatcgcgc cgaccaattc ccgtaaattt tgcggcggga
    53701 tgttggtggc catgcccacg gcaatccctg atgagccgtt caataaaagg ttgggcagtc
    53761 gggcagggag gacaagcggc tcttcgagcg agtcgtcaaa gttggggccg aaatccaccg
    53821 tgtctttttc gaggtcagcg agcatttcct ctgccatttt ttgcaggcgg gcctcggtgt
    53881 agcgcatagc agcaggggag tcgccgtcta cagagccgaa gttgccctga ccatccacca
    53941 gtaaatagcg catggagaaa tcctgagcca tgcgcgccat ggattcatac acagcagagt
    54001 caccatgcgg gtggtattta cccagcactt caccgacaat acgggctgac tttttataag
    54061 ccgtatttgc gcggattccc agctcatgca tggcgtataa aatacgcctg tggactggtt
    54121 tcaggccgtc gcgggcatct ggaagggcgc gggccacgat aacgctcatg gcatagtcaa
    54181 tgtatgccga gcgcatctgg gcgtcaatgt cgatggattc gatgttgcct gtattcaagt
    54241 tctcttctgc cataaaatat ccttaaaatg gggggaattc cgtcaaattg gcataaaaag
    54301 acacgaagat gggggagaac ttcgtgtctg cattagatga agaaaggtct ttatgatagg
    54361 gtaattatac cacccccata cctgttttac ctgtcacaaa cccaacaaaa aaagaccctc
    54421 aactgtgggt ctttttttgt gccgaaggag ggacttgaac cctcatgagc gtaagctcac
    54481 tacgccctga acgtagcgcg tctgccaatt ccgccacttc ggcttgcgat gcgggttgta
    54541 ttttatctca aatagttgga atgtcaataa gaagcgggca gttatgcggg aacaaaacca
    54601 cttgcctttg cgtttacttt gcaaatcgct tctgaggtta catatacatt acagtactgt
    54661 tgggggaggt ctctttttgc ggttttaaac gttatatagt taaacagaga aaggagttgc
    54721 aaaatgttcg tagtcgcagc agtggttgcc cttctgttgc tcgctctggc tggttctgag
    54781 ctctttgtag cccagtacaa ttcagaagaa ctcagcaata tgggagttca agagcaatga
    54841 tggaagacca tcccatccct tagcccgacg catgtttcaa gacgcgtcgg gcttttttgt
    54901 tggttaattc aggcgggcaa gtacgtcgct ggcttcgagc agcagataat ccacaccttc
    54961 atatttgaat tcagtgccgc tgtatttggc aaaaagcacc ttgtctttcg gtttgagttt
    55021 gatgctttca tcgtctccca ctgcgacaat ctgtcccgtt tgaggtttct ctttggcggt
    55081 ttcaggcaaa agcagtcctg atgcggtttt attttcctgc tccaatcctg cctaccgctc
    55141 ggtaggttgc gggagtttgg gatataatac acaagtctgc aggaaaagtg ccgataagga
    55201 taggaatacc atggaagtaa atgcccttca tacaaaaatt gacaaaacga ttcaggtcaa
    55261 ttctggcctg atcgtgttgg aagttccaaa cgggtttccg cgcagtgaat cgaacctgta
    55321 tttggttggt ttacgagggg atattttgtg gaaggctgaa aagcccgatg caaacaccct
    55381 gtattcgaag gtcaaactta atgaagatgg tgagaccttt tcggcttata ccctcaacgg
    55441 ccatgcctgc gatctggaat tgaagacagg gaaactgatc acctttacca gcattcaata
    55501 gccagatatt tgttcgtatc actgaaacgg tgacaaaaaa accacgttcg taagagacct
    55561 aatgacccaa cctccccttg gatttgtgct tgtatttttg ctgttcagcc tgttgttcct
    55621 ttcgaatacc tacaaactgt ggttcaaaac cgacacgtac tatcaagacc tttataatag
    55681 cctgaccaac gaaaagaccc cgcacccatt caagaattat ttcttaaggc ggctgcaaaa
    55741 ccgcaagcgt tgggaattgg agcagaagtt gttcagcctg gttggtttgg ccgctgttat
    55801 tggcgccgac gtgctggtgg tgacggcgta tttgggatga aataaaccac aaagtgttgt
    55861 gtgtaaaaga aaaacattcg tgacacttcg cggtaaaaaa gaatttttac aaggagtaca
    55921 acttgaaaat catcgcatgt atcaagcaag tacccgactc ggaagcgaaa gtaacggctt
    55981 cgggcgggca ggtctcatgg ggggacgcgc cgctggtcat caacccgttc gatgagtatg
    56041 cggttgaagg cgcgcttcag caaaaggaag ccactggtgc aacggtcacg gccttgtgca
    56101 ttgggcccga atctgcaaag gaagcgctca aacatgccct cgccatgggt gccgatgaag
    56161 ccatccttgt ttcagatgcg gcgctcaacg atctggatac cgttggcgca gccaaagtcc
    56221 ttgcggcggc gattcagaag attggcgcag atatggttgt ctttggccgc caaacccttg
    56281 ataacggcgc agggttgacc cctgcacaaa cagccagagt tctcgggtgg cccatgcttg
    56341 ggttggcagg gcagatcaaa gtcgacggtg gaagcgtgca ggttgaacga gtcctcgaag
    56401 aaggcaggca atctgtcaaa gccagcatgc ccgctgtcct cagcgtcgtg caaagcattg
    56461 gcgaaccgcg ctatccttcg ttcatgggca ttcgcaaggc atcgaaggca aacatccccg
    56521 tgtggtcttt gaatgacatt ggcgccagcg cccccgcacc catgatcacc cgcactgagt
    56581 tgatgacccc gcccgtgcgc gataccgctg ttgaaatgat cgcaggcgaa acccccgccg
    56641 agatcgccga aaaactcgcc gacaaaatca tcgcggagaa ggtgctatga aaacttttgt
    56701 ttatattgac cacttcaaag gcgacgtgca gcccgcatcc tgggaagcgc tcggccttgc
    56761 aaaaagtttc ggcacagcca ctgcgctggt gtttggcaca ggcgcagacg atgttgcgaa
    56821 agccgcgttg gaatatggcg cggatgaagt tctgctcgcc gatgattctt tgctgacgga
    56881 ttaccgcgct gaaacctatg cctcgaccct ttccgcgctc gcttcttctc atagcccgaa
    56941 cctgatcctg cttccgacga ccgctcgaac ccgtgaaatg gccgcgatgg ccgccattga
    57001 cttgaacact ggcgtgctca ccgatgtttc agggttggaa gcgaacggcg actcattcgt
    57061 tgcgactcgc cccatttacg aaggcaagct gatcgaaaag accgtgtgct ctggcaaacc
    57121 cgtcatggca accatccgcg gacgtgcctt cccgaagccc gagcgtgcag cgggcaagag
    57181 cggcacgatc acgaaagtct ctgcggctgg cgaagcgaaa tccaccgtgg aaggctactc
    57241 cgccgccgaa agcgccgtga acctcggcga tgctggcatc attgtctctg gcggtcgtgg
    57301 cgtttccaat aacccatcgc tcaccccgcc tgcaggcatg gacgagaaac aagccgaagt
    57361 ctggcgcgcc cagcagggtt tcgcgcttat caccgacctc gctacattac tgggcggtgc
    57421 cgttggcgca agccgtgccg ccgtagacgg tggctacatc acctatgccc atcaagtagg
    57481 ccaaacaggc aaggtggttt cccctgactt gtatatcgcc tgcggtgttt ctggcgcgat
    57541 ccagcacatc gttggcatgc gcaatgccaa agtcatcgtg gcgatcaaca aagaccccga
    57601 tgcacccatc ttcaaagtcg cccgctacgg cgtggtgggg gatctgttcg cagtcgtccc
    57661 cgcgttgaca gaagcgatga agaagaaatt gggaaagtaa gagcaagata cgagtaataa
    57721 aaattctccg aagccatctt ggcttcggag aatttttatt actattcgcg gaaacgtttc
    57781 agtggcagtc attttgagcg ctcatacccc gaaggctgct tccagaaccc atagcaggat
    57841 catgccgatg atgagcgtga ggaggacgtt gcgggttttc caggcaacga cggcaccgcc
    57901 aaggcccgcc agcaggcgaa agttttgcag ggaaatatcg agatggcccg tgggatgagt
    57961 cagcgcaggg aataccagtg ccgccagagc agccgcgggg acgaatcgaa gcgcacggcg
    58021 cagccagtcg ggcatttcaa tttttccaaa cagatagatg aatagaaagc gcagggtgaa
    58081 tgtccccaga ccgatggcga ggaataaaag ccagatggaa gcggcgctca ttgaatggtt
    58141 ccttttctgg cttgtatgct ttctgcccaa tatcccgcca tgatgccgag gaaggtggcc
    58201 agtaccagac cggtgttgta gggcaggcct ttggcaagca cggcaatgaa gccgcctgcg
    58261 agggctgcga tcacggtggg gcggtcacgt aatgcggcaa acatgagagc cataaaactg
    58321 agcgggaagg caaagtcaaa accccaactg gcagggacga gtgaaccgag caatgcgcca
    58381 aggatcgcgc tgatctgcca tgccacccaa acgatcaggg ccgaaccaaa ataataccag
    58441 cggtgattga tctcttcatt cgtagtcaca aaatgactca ggctgatgcc aaaagcctgg
    58501 tcggatagga tgtatgcgag caaagccttt cgccaggtgg atagtttttg caaatagggg
    58561 gccagggttg cgctgtacat cgtaaaccgc agattgatga cccaggcggt caataccatg
    58621 atgatccacg ggctgcccgc tgtcatcagt tggaagacca ccatttgtga tgctcctgca
    58681 aaaaccagca cagacattcc gattgtttcc atcaaggtca accccacact ggttgctgcc
    58741 acgccggtga tcatggcaaa ggggatcacc ccgatcagga tcggcaatga cccccgtgcc
    58801 cccgcgatga aatccttgtt catgcgaatc attgtttacc aacattccct tcaaaaagtt
    58861 ttggatgggt attctaccct caaatacggc aggcaaattc aatggagcag gggcgattgg
    58921 tatatccttc ccatttttgt tgactttttc ttttcgtccg ctacaatgaa tgaaactcat
    58981 tttggaggtt tggaatgcga tacggatcaa tgtaccgaat gttatttgtt ttgctttgtt
    59041 tttccatgct ttcgctggct tgtaatgcag tgacggaggc catgagcacg ccgcctccag
    59101 tggtgccgac agtattcaca ggcgggcagg aatcgcaacc gtctgcaaca caggaaacag
    59161 agagtgaccc tgagccgcaa gccacagaag cgggtgaaat tccgtttggc atcatcggca
    59221 ctgccccgga agatatcccc atcctgccgg atgcctctga gatgatggct tcccccaact
    59281 ctgtgatgta caccacccag ttatcgaaag atgaagcttc acagttctat gtcactgaga
    59341 tgaaaaaact tggctggtct cttaccccga atgcatccat taatggcgat gtggccaaca
    59401 ttctgagttt tacgatggat ggaaaaacag cttccattat cattggaacg gcgactggaa
    59461 aggtgaaagt gcaggttgtg atttctgaaa attaactgat gtgacccgta aaagtataaa
    59521 caacttttac gggttttcat ttttgatggg gaggatgttg aaaaaataag aaccttccaa
    59581 tgatgatcga ataacaatac gtcttttctt taccagcggg ggaatcccaa caatgatgca
    59641 agatattttg acgaatcggc caaagcatct ctgaatcctt gcaggagtct ttgcagatgt
    59701 tataagctgg ttttcgagtc tgtaaatgtt gcccgaaaac ctcgatgcta taatccgaaa
    59761 atcgttttcc aacctgatga atgctgacag gacaaaattc acagggatta aaggtcaaaa
    59821 ctgccaggta tcatcatgaa attccgcgcc aaattcattt gaaacatgcg agcctggatg
    59881 attccgggct cgttatttat taaaccatcc acccaaccaa actcatggaa aataaaactt
    59941 ctttacttct gatcgatgat gaccccgccc tgctggtggg gttggaggca gtgttgcgat
    60001 atgaggggta tcgtgtgttt actgccagac acggccgcac aggcattgat atcgcccgcg
    60061 aatccactcc cgacttgatc atttgtgatt tgatgatgcc gccacccaat ggcatggagg
    60121 tgctcacgat cctcggtcag gaccccagaa ctgccagcat tccttttatc tttttgacag
    60181 cccgaacctc ttccacagac aaggttcatg gcctgctttc cggtgcggat gattatgtca
    60241 tcaagccatt tgtgaaagat gaattgctgg cacgcgtaaa aactattttg aggcgcaaac
    60301 agaaagcacc tgtaccgtct tctgccacgg acaaggatga aattcaacac ctgagaaatg
    60361 agatcaccga aattctacgg aattctgaag tgaattggga aaagtttgta gacagcctgg
    60421 ttcatatgct gggattaagg aataacgaaa cagaagacca tgcctggcgg gtgatgaatt
    60481 tgacggaaaa gaccgccata gaacttggaa ttcatggggt ggaactcatc catatccgct
    60541 ggggggcgat cttgcatgat attggcaagg taggtattcc agataacatt ttgctaaagc
    60601 ccggtgaatt gaccaaggaa gagcgcaatg tgatgatgat gcatccgcag atcgccagtc
    60661 agatccttga gcctctgggg ctgccatcaa ccaccctgga tattcccctg tatcatcatg
    60721 agcgctggga tggaaaaggg taccctgacc atctggctgg cgataacatt ccactctctg
    60781 cacgcatctt ttccgtggtg gatgtatggg atgcattgac caacgatcgt ccctaccgaa
    60841 aggcctggtc ctgtgaaaaa gcaggagact acatccttgg tgaggctggg aaaagttttg
    60901 atccgcatgt ggtggatgtc tttttgaaaa aagttatttc gtattcgtaa atggaggagt
    60961 gctagtacat gtccgattct cgtaaaacca tacttgtcat tgacgatgaa ttccacttgc
    61021 tggttggagt aaaggctctg cttgagcatg aaggctataa cacagtgagc acaagcgatg
    61081 gaatggctgg cgtgcaaatg gcaagaaagg tgcagcccaa tttgatcatc tgtgatgtga
    61141 tgatgcccat catggatggt ttcaaggtca gggaaacatt gagtgaagat cctgttacga
    61201 aagacatccc ttttcttttt ctcagcgcga gggcatctca aactgataag ttgcgaggtt
    61261 tgaacaacgg cgcggacgat tacatcacca agccttttga ccatcgcgaa ttactggccc
    61321 gtatttcagc agtgttcaat cgctacgaaa aagggcaggg tgacatgcgc aggaaaatgg
    61381 atgaggagat cctgcgcttc gagcgggaga tttcacataa catcagccat gaactgcgca
    61441 ctcccatgac gcagatcctg cttgcgctgg atatggccct gcgaggcaag tacgaaaaca
    61501 ttgatgactt gaaactgttc gtggaaaccg ccctctcaca gtcgcataaa ttgaacggtt
    61561 tgatcgacga tctgatcttc ctgaataatt atgaccgtgg catggtcaat accttgcggc
    61621 aggaagttga cctcaaggca gatttcatgg agcccatcaa attcctgcag gatttgtatg
    61681 cggaaaagaa tatcaaaacc caggtcaaga tcgacccaac cgtgaccatc catgcgcctc
    61741 gccgtgagtt caagcgcgca attgggcatc ttgtggacaa tgccttcaaa ttctccaatc
    61801 caatgacaac gatcttgatc gatctggagg agaatgaagt gggaggcttc gtgttaaccg
    61861 tgaccgatta tggccatgga tttgctcagc aatattcaga aaaggtgttc aatcgtttct
    61921 tccagataga tcagggtgac tgccgtgcct ataatggcct tggggtgggg ctgacggttg
    61981 ctcgtatttt tgcaaggtcc cttggtggag atgtcaccat ttttcccagt accagggggt
    62041 gtcgtgttca aatgatggtt ccgcctggat ccactgaaat tccgtaaaag aagggatgtc
    62101 aaatgcatac aaataagact tttcgctggg cggtcatctt gctcctcctg gcttccattg
    62161 gtttaagcgg ctgtgcttca caaaagaaac cgatccgtat tgccatttct gcatgggcag
    62221 gggtggaacc agccgaactt gccgctcaat tgggatacta tgaaaaatat ggcgtggatg
    62281 tggaacttgt gcgtttctcg gcgtacagtg attcactgga ggccctgcgt gacgagaatg
    62341 ttgatggcgg catgcatacc ctggatgact cactgcgaaa tatggccgca ggcagggatg
    62401 tgcgcgtcgt tctgttgacc gattattcct tcggtgggga tgggttggtg gcaagagctg
    62461 ggattgagaa tctgtctgat ttacgcggta ccaagatcgg ggttgagata ggaaccgtcg
    62521 gccatttatc cgctttgaag attcttgaaa aagcgggagt tgcggtcagt gatgtgacct
    62581 ttatcagtat ccccgcctgg gaaattcaac aggcgatgat cgacggtcag atcgatgcag
    62641 gggtgacatg ggagccgtac ctgaccagca cggctgaaat gatcggtgga aaagtgttga
    62701 tcaccagccg tgaataccct gagacgattg ttacgaccat gactttcaat gccaaactga
    62761 ttgacgccag acctgacgat gtgcaaaaga tcgtggctgc ttattttgat gcggtggaat
    62821 atattaagca gaatccgcag gaagcctaca cgatcatggg caaggccgaa ggcatcagcg
    62881 ctgaggaatt ttccacacat gttatgggta tcaggtatct tgatttgagt tcaaatgcag
    62941 aattatttgg cgtgaattcc gatggtcgtg tatatgccgc tgctcaggaa atcagcgtgt
    63001 tcctggccga caacaatgtg atcactacta tgcctgatgt tcaacaaatg ctggagccga
    63061 cctttgttct aaccttgttc aaggacggcc aatgagcgag atcagaataa actgatatga
    63121 attctttaaa gacaaaactc accatttatc aggggttggt tggctttgct gggattctgc
    63181 tgatcgtctc gatcacctgg ctggtttccc gccagcggat tttgagtaat gccattgccg
    63241 aggctgaaaa cctttcagac cagatcgcct atagcatggt ggtcattgcg gcccagggcg
    63301 gcgatgctac gtttaattac caaaggctga tcgagaagac atccaccctg aataatgtca
    63361 gttccatcaa ggtgctcgat gagaagggcg ttattctggc tgacaatgat cacagtcagg
    63421 tggggcaggt gttcaactct cctctgttgg aggattccct gaagaatcaa cgcaaggaac
    63481 aacaaattga gggaaggtca ttgatcgtgg tgagaccctt gcgcggagag acctacacag
    63541 ccaatctcaa tgatgtgacg ggttttttat ggatcgagtt ggatttcacg cccgcctatg
    63601 cccgcgcaca ggaagacatg tccctgatcc ttgcggtcag tcttggaggc ttcctcctga
    63661 cgttttattg gtactaccgt gtgacccaaa acggaatcct tgaccgctta agtgtccttt
    63721 ccagcgggtt gatcaaggtg gagaatggtg atctgaccca gagaatttca attcggaaat
    63781 tgtttggaag cactgatgag atcaatgaac ttgccagcca gtttaaccgc atgaccgcca
    63841 gcctgcacca aaagctgact tttgaagaat taacctcaca tttgtcggcc aagttcatga
    63901 atgtgccgtt cagcgaaatg gatacggctg tccacgatac gcttcgcagg ctgggcgaat
    63961 tgtttaaagt ggatcgcacc tacatttttg aatttaccga tgatggcctg gccatgaaca
    64021 atacctttga gtggtgcgca aacgagatca ttccgcagat cgacaacctg caaggggtgc
    64081 caagggaaat tgtgccctgg tggatgcaat tgcttgaaaa tggcgagatc atcattattc
    64141 cgcgtgtatc agaaatgccg aaagaagcgg cgtctgaacg tcaaatcctg tctgagcagg
    64201 ggattcaatc cgtcctggta acgccgatga catctgacca cggtttgttc gggtttatcg
    64261 gctttgactc ggtggctcat gagcgcaact ggaccaagga agaagttaat ctgctaagca
    64321 tgttatccgg ggtcgttgcc aatacgttga ttcgccaacg cagccagcgt gaagttgtgg
    64381 aggaaagaga ctttgccctt caagtgatgg aaaccgttgc acacgggttg atcgttgccg
    64441 acctggatgg cagtttggaa tataccaatc ccgcctatgc caagatgttg aaatatacag
    64501 cccaagagat cacaggcagg aaaattcagg aattcatgca catcgaagat ataccgcagg
    64561 atgaaaccca aaactcatat acagcccaga tgcgcctgat tgccaaagat ggaaccgtgg
    64621 tcaatacact ggtaagccgt tctccgcgca tcaaggacgg tcacattaaa ggcagcattg
    64681 gtgctgtggt ggacctgacc gagcaattgc ttgccgaaga aaaactgcgt caatcagaag
    64741 cccgcaaccg cgcatttttg aacgccattc ccgatttgat tttccgcatg gatgaggcag
    64801 gccgttttat tgatttcaag gcaggagacg acagccgttt ttacattccc cccgaagaga
    64861 tcatcggcgc atccgtggca gatatccttc cagccgaggt cagtaacatg acccttttag
    64921 ccatcaagaa ctcactgcaa acctcaaagc cgcagagttt tgaatacaaa cttcaaactc
    64981 cctcaggttt gttcacgtat gaagcgcgtg ttgccgcatc ctcgcctggc gaggtcattg
    65041 ctgtcattca cgatattacc gaaagggcgc ggcttgaaca aatgaaaacc gattttatca
    65101 atcgggcatc gcatgaatta cgcaccccgt tgaccacagc cttgttgatg gtgaacctgt
    65161 tggaaagatc ttatatcaaa cccgaggagc gcgaagaata ctggcagatc cttacccagg
    65221 aattaaaccg cgaacgcatc attcttgaag atgtgcttac tgttggacgg ttggaggctg
    65281 gcaagtaccg cattgccgat ggtctcgtgt ccatcatgcc tgtattgaaa agtgcaatag
    65341 acagtatgca attgcaggcg agcatgcgga atgttgagtt tcaagttgat gccccggaaa
    65401 cccctccaga cctgcgcggc accgaagaag tgttcgccag aattttcagc aacatcgttt
    65461 caaacgccgt caaattctct cgcccggaaa ccagcattca ggtccgtgta attccgaatg
    65521 cagatggcat gagtgtggaa gtgcaagatc atggcatcgg catcccacct gaagaccttc
    65581 cacatattac ctcccgcttc ttccgcggca cgaacgcaac cgaaatggag atcccaggca
    65641 gtggcattgg cttatacatc atcaaggcgc ttaccgaaga actgggcggc aaattgggaa
    65701 ttcagtcgaa attaaatgac ggaacgacga tctcattgtg gttcccgatt cccagtatca
    65761 agaaagatga aaaccccgca tcacactccg ccgatgattg gtccggcgaa aaggattcgt
    65821 cagcgcctaa agtggcttct tcatgatccc agcagctgct tttgcagcca ttctttgcgc
    65881 tcatgagtgg catctgcatt caacccatgc cccgaagtgt agaccttcat ttcctttggc
    65941 ccttgtgcag cggcgaaaaa ttcatcggca cgctcaagtg gaacatggaa atcatcattg
    66001 ccaaattgaa agaggatcgg tgcaggggcc aattttgaaa catgcatgat cggatcgatc
    66061 tcggccattt ggttgatgaa tgcctctcgg gcgtcgcctt ccagtttggg cagatacagg
    66121 taccagtcag ggaaacgagg cgtggccgcc atgatgacgt aatgggtggg gcgtcggtcg
    66181 gcgctgccag ccagaacacc atacatcccg ccgaaatcgt gccccacaca ggcaaaacgc
    66241 ttgggatcca ttttgggctg ttgagaaaga aaatcgataa aacggcgcag gttcacgacc
    66301 tcttcgatcg aatttccagg gtcatcggct tgtgtgcgct ttaggaaaaa atcagggtcg
    66361 gaccacagag tttccaccgt cagacagatc acacctttgc gggctaattc ctctgcctcg
    66421 ctgacaaatt gactgcgatt cgaatcgcgt gcctcaggct cgtaccaatg gataaaaagg
    66481 atggtgggaa aaggcccttc accttcaggc aggaacaact ctgccatgcg ccgatacccg
    66541 aagggcgttt gaaacacaag caattgcttt tccaaaccct cttcttttct ctgtgccaac
    66601 acacggacat ccgtagggat aagtttctgg tggttttgca tttcggtgaa catgaaggcc
    66661 tccattcaat gagttcaatg ttcgaattat atgggtttcc catattttga cacccttctt
    66721 atggagggta tgtttgtgcg ggcatatccg ctttgactat cttataataa gccctgcttt
    66781 agcagacctc tagtaaaagt ctgaaagatt aaccacggag acacagagaa aannnnnnnn
    66841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    66901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttagcag acctctagta aaagtctgaa
    66961 agattaacca cggagacaca gagaaaaaaa taaaaactcc gtccgcagga tgctttgcac
    67021 tgcctcagtg tctttgtggt tcaaaatggc ttttcaaaga tattccatac aacttatgca
    67081 agtggttaaa tataaaaatt attgtacaaa ttgatttagg ataggtatat tgtgtcaaaa
    67141 ttgctttccc gtttttcgta ctcaaacctc aaagaacaac taatgaagat ctggcctgtg
    67201 gtccttgttg ggatttttgt ttctttgggc ggggctggta tcaccgtatt ggtcgctgca
    67261 ttgaccgata accccatttg gaaactggcc aaagacccag ccgaagtaat gcagtttccc
    67321 tcctatatcg gtatgctttc caattggggc gtccttttgt ggattgcttc agcagtgatt
    67381 tgcctttttg gtgtggccgt tttgaaacaa tacgaagtgc ccaaagatac tttgaggttt
    67441 atcacaggtt cggggctgtt aagcctgatc ctgggaattg acgatctcta ccgtctgcac
    67501 gacaaaatat ttcccaaaat gttccacctg cctgaaaagt tcttctacat actttatatc
    67561 tttatttttc tcctgtatct ggccatgttt gccgcccgta tcatggaata tgaatttttg
    67621 ttgtttggcg ccgccttttt tttgctggtg gtttcccgcc gtattgtggt gatcattccg
    67681 ttcttcaacc agttcatgac cacgggtgac atgttgaaat acttcggcat tgttttctgg
    67741 ctggcattct tttatcgaac ggtactgcat gagatcacca gcctgataaa caaaaacaag
    67801 actgcttgac agcccattga ccgcgcgtat aattcagccc aagtagaccc acgcgaagac
    67861 aagtatctgc ctcccctttc agagagccat cggctggtgc gagatggcag gccaagcagt
    67921 gaaatcccct tcgttatgtc gctcttgcag ggatacgcaa taattgctat cgtaagagag
    67981 tcgggtgcgc ccgttatcgc gcaaatcaag acctccaccc agtcttccct aaccatttag
    68041 ggaaggagat gaaatcggga aggggtaaaa gcaggtggca ccacgagacg ccccctcgtc
    68101 ctgcaaatgg attgggggcg tattctgtta aatgcacatg gaggtgcaac atgttggata
    68161 tcaatttaat tcgcgaaaca ccggatgttg tccgcaaggc gttgaaagac cgccagatgg
    68221 acgcctctcc ggtggatgcc attcttcagt tggacgaaaa acgccgcacc ctgcttggtc
    68281 aggtggaaac gctcaaggca gagcggaaca ctgtctcaaa ggagatcggg caaatgaaag
    68341 acgcagcagc ccgacagtcg aagatcgagg cgatgcgggt ggtcggagat cagatcgcag
    68401 agttggacaa gcaggtcgct gaagtcgaga ctcaattgag cgacctcaca tccgcgctcc
    68461 caaacatccc cgatgctcgt acaccctacg gcaaagatga cagtgaaaat gtgatcttga
    68521 ggacggtggg ggagccgcgt aaatttgatt tcaaaccgct tccacactgg gaccttgggc
    68581 ctgctcttgg aatccttgat tttgagcgcg ggacaaagct gaccggttcc cgtttttatg
    68641 tgttgcaaaa agcaggcgcc cggttacagc gcgccctgat cgcatggatg cttgacctgc
    68701 acacccgcca gggctatcag gaagagtacc tgcctttcat ggtgaagacc gcagttgtgt
    68761 tcggtgcggg gcagctgcca aaatttgcag ataatctgta caaggatcat gaagaagatt
    68821 actactttgt gccgaccgct gaagtccccc tgaccggcct gcacatggat gagatcgtcg
    68881 aagaagccac gctgccgaaa ttgtacacag cctacactcc atgcttccgc cgtgaaaaga
    68941 tgagcgcagg ccgtgatgtg cgcggaatca aacgcgggca tcaatttgac aaggttgaga
    69001 tgtacaccta ttgcaagccg gaagaatcgg aagcccagca tgaaaagatg ctgaaagatg
    69061 ccgaagaaac ctgcgctttg ttgggtgttc cgtatcgcgt gaagcgcctt tgcaccggtg
    69121 acctcgggtt tggttcaacc atgacctatg accttgaact gtgggcgcca ggctgcgatg
    69181 aatggcttga ggtttcttcg atctcgaatg tgaccgattt tcaggcaagg cgcgccaata
    69241 tcaagtaccg ccccagcgac ggaggcaagg cgaaattcgt acatacattg aacggatcgg
    69301 gtttgggtct gccgcgcacg ttgatcgccg tcatggaaaa ttatcaacaa gccgatggat
    69361 ctgtggtggt gcccgaggtt ctgcgcccgt ggatgggcgg cattgatgtg atcaagccgt
    69421 aaatagaatc gacctgctcc tctcccaata tccgcggggg aggggcaggg aacatggagg
    69481 aagccatatg gaccctgaag ctctaaaact ctttgcagaa gtatttctgg aagtgaccac
    69541 gttcgttatt ttgcttttcg gtttgtttgg cttgctcatt cccgttttcc ccgggttgac
    69601 agtgatgtgg atcgccacac tggtctatgc tattgtgcgg ctttccgtgg gaaatatgac
    69661 atgggtgggt tggctgcttt tcgtggtcat caccttgttg atggtcggcg gtaatatcgc
    69721 ggataacatc atcatcgcca agcacgtgcg cgataaaaac gtaccctgga gctcgatcat
    69781 ttgggcattt gcggcgggga tcgtggtcag tttgttcctc accccactgg ctggaattat
    69841 cgcctccccg atcgggttgt atttggcgga atggtatcgc ctcaaggaaa agcaacccgc
    69901 ctttgataac accaaagcat ggatgacggg ttgggggtgg tcactggcgg caaggtttgg
    69961 aattggtgtg gtgatgatcg ccttttgggg cttgtgggcg tggatataag agtagcgcgg
    70021 acttcagtcc gcttttgccg cggattctgc tgaactttgc ggaatccgcg gcaattctat
    70081 tttaatggtc agtcatcttt atcttttaat tttttctgcg catcgcgtat ggcgagaaac
    70141 aaaattgccc caacaaagga tgtaaaagca agtattgcaa acgcttgcca gaaatagccg
    70201 ctaaagagca ggtacacgga aaatcccacg gggatcaaaa acagaatgag agcaaagccc
    70261 tgttgaaaag caggatccaa actttcgttt tgataagggg agacaggttt atatttccat
    70321 tttggcatga gataccacgg ggtgcggcga tacgcatggt cgctcttcca cttcacctgg
    70381 cttaggtaat cttctgcatc ggatggttca aaattcttgt tttttgccat cgtaataaga
    70441 cccccttact ttttcgcttc ctgcacggcg cggatgatcg cttctttgaa ggtgtcgcca
    70501 gcgttgtctt tgccgagtgg caggaagatc atgccctgtt cgatcacatc tgtgaatcgg
    70561 cctgtgatgc tcaaacctgt atcggggatc agggattgga aggaactctt ttcataatcc
    70621 tcgatcacaa gatcacgcaa ttgcatggac ctgacgtgta ctggtttcgt atccatctgg
    70681 tcggcgagga tcagccagcg gtttgtcagg atgacggttt ccaccagcac ttttggacct
    70741 ggcccgctga acaatccctt tttgatcttt tcagaggttg agacaatgca gtgtgtaacc
    70801 ccgttgagaa tatcgccaaa attgtgagtt tcgatgtgtt gatgaacggc ctgcatgata
    70861 tcaggcgaaa tgtgtgcaaa atttatttcg acggttgaac ggttatagtc acccattttt
    70921 cctcgcataa aaatcacagc cccaggatgt tttgaatatc ctggggctgt gatgattaga
    70981 aattcacaga ctttgaagcc agtgcagcaa gcaatttgac cgtgttcttc atatcgtcgt
    71041 aatccaccat ctcagaagaa gagtggacat aacgcactgg gatggatact gcccccgtca
    71101 tgacgcccgc ccgcgaaacc tggatctccc gcgcatcggt gctgcccacc aacagaacct
    71161 cacgttgatg ggggatcttc gccttttcag cagtttggag catccaatcc accacccgcg
    71221 ggtcagataa catacccatg tcgcggaatt ttacacaggg gccctttcca agctccatca
    71281 ccatcttgag cgaactgggc gtatcgccag tgggggttac atccacagcg ataccgatat
    71341 cggggtcaat gccgtaggcg gcggtacctg cgccgcgtgt gccgacttct tcctgcgtgg
    71401 tgaacacgaa atacaggtca ttgggtgtgg atttgaccgc gcgcatcgct tcgatcactg
    71461 ccaccacgcc tgaacggtca tccagtgatt tggcaacgaa gcggttgccc atttcaacga
    71521 atggacgttc gaatgcagcc acatcgccca ctttgacagg gcaatccttt ttgctggttg
    71581 cacccacatc aatatacatt ttgtcgatgg gcagccaatg atccatctgc tccaaacggt
    71641 cgtaccctac aacccctgag accccattca aaaagcggac acgcgccccg agcgtataac
    71701 ggccaaacgt gccgccgata ttggtaaaac gcacaaaacc gtttttatca atatggctga
    71761 caatgacgcc gatttcgtcc atgtgcgcgg cgaacatgat ctttttggga ttcttcgccc
    71821 cgctggcggg ctttttgcgc gcgatcacat tgccgagcgc atccacgcgc acttcatcgg
    71881 cgagcggttt cacctctttc aatacgatct tgcggatatc atcttcatag cctgatggac
    71941 caaaggattc agtgagtgtt ttgagcaatt gtttcatcgg ttaatttcct agggaaaatt
    72001 tttcgccagc actgtaactg cggctttcat tctttgtgaa cacatgcgct ttggcatgcc
    72061 caagcacgat ccattcttcg ttttgcctgc cgacgatggc ggtgttttcg tcaatgccga
    72121 tcatcatttc gcccgctttc aaacgtccgc gcagggcaga gacgagcggc ttgccgaaca
    72181 atggaatggc gtcaaaatgc ggcatgacga acgcggctgc gatcgaacca aaggctggaa
    72241 tggaacgcat ccccatggcg cggaaatcgg ggatctcccg cccaaggatc attgcgcccg
    72301 ccgaacagcc cgcatacaca gctccacgcg cccaggcttt ttgcgccgcc tgccagacga
    72361 gactatcttt catggtgcgg tgcagatacg agggatcgcc gcctgaaaag tagacgatat
    72421 ccgcatcttc cacaaccgag gcatggttca agtcattggc tgattctgcg tcgatgacag
    72481 gaacagcttg aacgtcagcc ccgaggcggg tgaaatgctc cacgcccatg ttcgaccagc
    72541 ggtttacgct tgcgtcgcct tccctgcccg cagcggttgg cagacaaacc acacgcggct
    72601 tgcggtcggt cactccgcag tttgccagca gatgacgatc aacatcatcc atgaccgaaa
    72661 gatattcacc agaacccaat aaagcgatca gtccgttcat cttttacctt ttaagtagat
    72721 caggtgacat ggctttcaac gcagtgtgca gcagatttaa cgtattcttc caatcgtcca
    72781 cacgtgaaac gctgatgggg gaatgggtat agcgatgcgg cactgaaacc gaaaccacag
    72841 gcacgcctgc gcgtgtcaat tggatcgcac ctgcatcggt gccgccgccg ccaggctggc
    72901 gcagttgatg gggaatcttg tttttgaccg ccgtgtcttg aagaaatttt acaagacgcg
    72961 ggtcgtcaat tgtggatgaa ttcgccacat aaatggcagg gccaagccca agctgcgtgt
    73021 tgtattttgt gttttcattt ccatcataat cgggcaggtc gcgcgcaggc gtggaatcga
    73081 cggcaatggc gagatcaggt tcgaaataat gcgccgccac ttttgccccg cgcaagccga
    73141 tctcttcctg cacactgaac gcaaggcaaa gctcaatgtt ccttggtgcg tttttcaata
    73201 actcgatcaa gatcgccacg ccgatgcggt catcaatgga tttggacatg atggaaggcc
    73261 cgaccttttt gaactttgtg gcaaaggtgg cacggtcgcc gactttggct ttgccttcga
    73321 ggccgagatc gatcttcaat gagtcaatgc tgagggaatg ttccatttct ccgctggagg
    73381 cgaggtgaat gggttttgcg ccgatcacgc ccaggctgtg atccttgccg acgatgacct
    73441 gtttgccgac gagatgacgc gaatcaatgc cgccgaccgc ttcgaattgg taaaagccat
    73501 cgccttcatc gctgacgatc ataaaaccca cttcgtccat gtgggcatcg agcagcaccc
    73561 gcagcggttt tttgccagaa gaggccgccg catgtttgcg caccagcaca ctgccgagcg
    73621 catccacttt gacttcatcg gcaaagggtt tcacttcttc aaggacaatg cggcgaactt
    73681 cgccttcgtc ccctgaaaca gacatggcat tgcatagttt ttcaagcagt ttgatttggg
    73741 ttgtgccaat tgaaagcata ttgattaatc ccacacgatt ttggtaagaa aatcttcttc
    73801 aagcgaggtg ataaattcgg caagcaaacg gccagcacgc tgaatatctt tcagcgcaac
    73861 gacctccaca ggggtatgca tgtatcgttg cggaatgctg accaccaccg tagggatgcc
    73921 ttccgccgtc aattgcatgg catctgcatc ggtggatgac atttctggca tggcttcatc
    73981 cttaacgggg atttccaccc tttcggccac ttccttcatg cgtttatgca aaaacggatg
    74041 gacactcggg ccgatgccga gtgtcagtcc cttgcccagc gggaaggttt gatatccgtt
    74101 cgcgcctgca cctttcccaa aggtcatatc caatgccact gcaatggtgg ggcgcaattg
    74161 aaatgccgat gtgtacgcgc ctgcgtaggt cacttcctct tgcacagagg ccagcgccca
    74221 cacatcccac gcatgtgatt tggattgaag ttcttcaagg cagacggtaa gcgcggcgac
    74281 ggaagcgcgg ttatcaagat tgtgcccgct cacgagccca cctgacattt caatgggttc
    74341 tgtgttaaag gagactcgat ccccaacgcg gactcgcttc gagacgtcgg caggcaggag
    74401 gcctgtatcg accatgaggt atttcaagtt gacagcgcct tcgcccttgc cttcgggcag
    74461 taaattgcca ggcggcatga cgataacccc atacagttct tcgccgctcc ccgtggcatg
    74521 gacggtgacg ggtgtgcctg gcagaacacg atggtcaatg ccgccgatat tggtgatgta
    74581 caaaaatcca tctacaatgc gggtgaccat caggccgatg gcatccatgt gggtggcgat
    74641 gagtacggaa gggcgggggg atttttcggg tttggaaatg cctttcgaag gcgtaccctt
    74701 tttcagtcca tgcaaagaac cgagtttgct tcggctgatc tcatccacca gcggagtcca
    74761 ctgcttttcg atgaggtccg ctacggggga ttcatgtcct gaaaggcctg cgacagaaat
    74821 gattgatttt agaaatggga gaatatcgct cattggtaaa ccctaatggc tttcaaagaa
    74881 ttttgtgttg cgtgcattgc gttatccccc aaacgcagtg ggagatggag atggggtgtt
    74941 ggttggggtt ggggtcagtg taatcgttgg cgtgagagat ggtgttaacg tgatggtggg
    75001 agagggggtg atggtgagtg tgttggttgg ggtggaggtg agagttacac tgggggtaat
    75061 gctgggggtg agtgtgttgg tcagggtgaa agtgggtgta atggatgggg tgagtgtgaa
    75121 cgttaaggta accgtgttgg cctggaaggg ggtgtaggtt ggtgtggctg tttgtgtcaa
    75181 tgtgttggtg acaatgacga tttgtgtgat ggtatttgtg atgatgggag gggtgacgct
    75241 ttgagtggga gtggaaggcg tgccagtgac cgtgaggatg gtggtggtgg gagtggcgtt
    75301 cggttttaca tctggggcca gtacaagaaa gataaacccg atgcaatagc agggaatggt
    75361 ggcaaggatg accgcaaggg ctctcattcg gttacgagtg cggggtgctt cggtttcaga
    75421 catatcgggt cgattataat ccaacatcat cttatgattc ctctacgtct tcgaatttca
    75481 ggctttcttt catatcgcga ccctgttgaa ttggattttg actcgttcga cctggcttgt
    75541 atttcaggtc ataacggcgc gggtaaatca tccttgctgg atgcgttcac ctgggttttg
    75601 ttcggcgagg cacgcggaaa aagcgccgat atcatcaact tgaactcgga tgtcaaagct
    75661 gcggaagtgg cgttgacctt caagtacgaa ggcaatgtgt tccgtgtgca aaggacactg
    75721 ccgcgcaaca aaagcaccct gctggagttt caggttttgg acgagggcga acagaaggca
    75781 gaccttgaaa gcggaacgtg gaagccgctc actgagaaaa cgacccgcga tacgcaggcc
    75841 cgcattgaac agacactgcg actggattac gataccttta tcaatgcctc cttctttttg
    75901 cagggcaagg ccgatgaatt cactcaaaaa aaggcgggtg accgcaaagc cgtgttgagt
    75961 tccatcctcg ggttggaagt gtgggatgaa tacaaagacc gtgccgccga taaaagaaag
    76021 atcgtcgaac gcgaggtgga tgaaattgac ggccgcatcg ctgagatcga ttcagaattg
    76081 gccgaagagg atgcgcgcaa agcgcggctc gcagagcttg aaaccaccct tggccagctt
    76141 tccacttcac gtgccgcaca agaggcggcg ttggccaata tcagaaaaaa tgccgacctg
    76201 ctgaatgagc agcgtaagct gactgaggcc ctgtccactg ctttggagcg cgcacgcata
    76261 gcacttttgg gcctggaaaa acggctcacg gagaaggaat ccgaacgcac cgcatacaca
    76321 gacctggttc accgcgcaaa agagatcgag tccagttaca aagcctggca aaaaacgcgc
    76381 aaggaattgg aatcgctcga tcaaattgcg atggaattcc gagagcatga aaaagaacga
    76441 acaccactgc tcgagcagat cgccactgaa aaggcacgtc ttgaagaaga aagacgaagt
    76501 ttgctggttg agcaggagga gatcgaaaat caaagcgctt caatgggtga cctgaccgcc
    76561 gaagtggaaa aggcccgcac cgccctgcaa gatgcggaaa tccgaatcgc agagcggacc
    76621 gaattggatt ctgtacgaaa cgatgcgcgg gagcgtcagg ctttcttgaa agcagagaat
    76681 gacgcgctca aggaaaacat gaacagcctg aaggaacgca tggattccct tgatgccgcc
    76741 gatggcgcga cctgccccct gtgcggacag gaattgaccg aaaaacatcg caaggccacg
    76801 gtcaaacaat tggaagccga aggtaaacaa aaaggcgata aattccgcgc caataaaagc
    76861 gaaatggacc agcttgagaa aacgctggcc gattgcacgg cccgtcttgc aaagctcact
    76921 ggcgcagaga acgaccgtct gaaattttcc aataccattt cccaacttac agaacgcatc
    76981 gaaactttgg gtgtggttgc aaaagaatgg gagaaatctg gcaaaaaacg gctggccgaa
    77041 gtcactaaag ttttggaaaa agaatccttt gctgccgatg cgcgcaaagg tctggcgaaa
    77101 ctggataaag aactcgccaa acttgggtac gatgttgcca cccatgaaga aacccgtcag
    77161 caggaactcg agttgcgaga agtggaagag gaataccgca agctcgaatc ggcgcgcgaa
    77221 ttaagcaaac ggatcgcaaa agaaatcgac gaactggaag aggaaataaa aaagaagcaa
    77281 gcggaagtgt cggaaagtga agaagcccat cgcgcggctg cacaggcttt ggctgagtcc
    77341 gaatcgcatc acccgaattt ggatgatgtc gaacgcgact tgttcgcatt gcgcgaagac
    77401 gaaaaccgaa ttcgtgacca ggtcggtgcg gcgcggcaaa aagtggaggt gcttgccaca
    77461 ctccgcgggc gcaaggccga ttatgccgtt cagcgtgaag aacttaatca gcagatcgtc
    77521 cgctacaaaa ctttggaacg tgcctttggc aaggatggtg tgcccgcctt gttgatcgag
    77581 caggcgcttc cacagatcga acaaaaggcg aacgacctgc tcgaccgctt gagtgacggg
    77641 caaatgtcca tccgatttgt gacacaggcc gaatataaag acaaaaaacg agacgacctc
    77701 agagaaacgc tcgatattca gatcagcgat tcggcaggcc agcgcgatta cgaaatgtat
    77761 tcgggcggtg aagccttccg tgttaacttc gccatccgtc tggcgctttc tgaaatcctt
    77821 tcccaacgca aaggcgcccg cctgcaaacc ctcgtcattg atgaaggttt tgggtcgcag
    77881 gatgcgcaag gccgccagcg tttgatcgaa gccatcaacc tcatcaaaca agattttgcc
    77941 aaaattctcg tcatcaccca tcttgatgaa ctcaaagatg cctttccaaa ccgcatcgaa
    78001 attgaaaaga ccgagcgggg ttctgccgtg aaagtcgtat agaaaataaa acccactttc
    78061 cactttctac tttccatgtt ttcccatgcc ttatttcatc acatcaacca tcctctttgt
    78121 cgcagcactt gtcatcattt actggatcca caatcagtat catcttgata ttgttgttac
    78181 atccgccaat cctcccgccg atgcgccgct gttctcgatc tgcatccctg cccgcaatga
    78241 agaaaacaat atcagcaggt gtgttgaatc cgcacttgcg caggattatc cgaacatcga
    78301 agtcattgtt ttggatgacc gctccaccga tgcgactctg actcaattaa aagaaattgc
    78361 cgcccgtgat tcccgtctcc acccaatcag cggatctgac ctacccgcag gctgggcagg
    78421 taaaccccat gcattgttcc aggcctcggc agtggcgcac ggcgactggc tgtgctttgt
    78481 agatgccgat acctttcttg aacctggcgc catttcagca tgttatgcaa aagccctcga
    78541 aaccaaagca gacctgttta ccacaatgaa ccaacaagtg cttggttctt tttgggaaaa
    78601 ggtggtcatg cctctggtca tgaccgcact ctcagtggga ttttctccgc gcaaggtcaa
    78661 cgacccaacc cgccgcgatg ccgttgcgaa tggacagttc atcatgatcc gtcgtgcagt
    78721 gtatgatgcc attggcgggc atgaaaaagt gaaagaccag atcgtggaag ataaagccat
    78781 ttctgaacag gtcaaatgga atggtcatcg tctggtcgtt gcggatggtt cgcgtttcat
    78841 ccgaacacgc atgtacactt cccttgaaac catgtgggaa ggctggacaa aaaatatcta
    78901 ccttggcttg cgcgaccatc ctgccatgtt aatgctcggc gcatttggtg ccacgcttgc
    78961 cctgatcgca gccttgtttc ttcccatctg gccaatgttg ggtttgggtt ggtatctgca
    79021 aggcggggga gcattggcga tttgggttat tcttgaggca ttggttgttt ggggtgcctt
    79081 attgctggca cgtgctcggg tggcccgcaa aatgttcatc tccgcctggt atgcatggac
    79141 aacccctctc ggtgcaggtg tctttgccgc catgatgctg gcctccgcct ggaaggtcat
    79201 ttcagggcag ggggtcacct ggcgcggcag aaaatatcac cctgccaagt aagtgatctg
    79261 gcttgggcaa atgtttggtg aacgcgtaat tcggcctact tttgcgcata tccttgacga
    79321 atcctcttcc cgcgtataaa atccaagccg ttgttccctg cgcctccgcg aggaggcgcg
    79381 tttttccgcc aaaaacaaaa gagtatgcca tgaacgaacc ctctgcaagc tccgaaaatg
    79441 atcaggttca agtaagcaac ctctcgcaag tggatgttga gatggttcag gctgaattgg
    79501 tgcggatgca tcagagctct gcacaagaaa ttcaggcaga agatgtggag ttgggaatga
    79561 gcgccgcggc agaggtgaag gctgccaatg ccacggtccg tgattcggtt gttggcttgc
    79621 ttcaggcaac agatgcaagc atgcaggata atctcgtagg agttgcccgg gctgagacca
    79681 tgtctttgaa tggcggggca ggcgtggtcg tggcaggaag cgtcgaattt ggcaatgcct
    79741 atgctggcgt tgtggcagga cgtgaagtcc gcggtgagaa gatcgaatcg gtcatcctac
    79801 tttcgcggaa tgtacatggc aatgtcacca ctgtgatgga cacgcgcggc gctttgatcg
    79861 caggtttggt cggcggattg ttcgcgggaa tcatgttgct gctgggccgt atgctttttg
    79921 gccgaaaata atttgaatgt tcagcggaat aagccgcttt gcagaaattc atttaatagg
    79981 gtgaaggtaa ccgcgcgatc gcggtgagag gcgcccaagg agtaaagatg gaaaaagtag
    80041 tattgaaggc ttcaaaacgt gatgtggttg gcaagcaggt gaaggccctg cgccgcaatg
    80101 gtcaattgcc tgcggtgatc tatggccgtc acgtggaacc gattgccatt tcactggatg
    80161 ctcacagcac cgggcttgca ttcaccaagt tgacctcatc cacattggtg acgattgatg
    80221 tagacggcac cgaatacgcc gccctcgtgc gcgaaaagca gcgcaattac atcaagggaa
    80281 acctcacaca cgttgacttc ctcgctgtgg atctgaccga aaagatcacc accaaggttc
    80341 acctcacctt taccggtgtg gcccccgcgg tgaaggatta cagcgccgtg ctcgtacacc
    80401 gcatggaaca gctcgaagtg gaatgtttgc ccaccgacct gcccgaacgt atccttgtgg
    80461 atatttcatc catcaaggcc attggcaaca gtatccgcgt ccgcgatatt cagcttcccg
    80521 ccaacgttgc tgtgttggaa gatgaagatg aaatcgtgat gatcgccacc ctggccaagg
    80581 aagaaaccac cgaagccgct gctgccgctg gtaccgaagc cgccgctccc gccctcagcg
    80641 tggaacgtgg caagaaagac gaagaaggcg cagagaagaa gtaagaatct gtgtgaatca
    80701 aaaacccgcc atccaacgga tgacgggttt tttgttttgt gtgcatcaga atgtctgacg
    80761 gaacttggtc acattcttat ggatatagca tgacttccat ggtgtagata taaacatact
    80821 ttttgagatc ggggtcgctg atgctgatat cggggttgtc ctggcggatg ctgaggcgtg
    80881 ccagagtggg ttcagtgacc ttgtagggca gggtggtttc aaattcctgt gtttcgatgc
    80941 cgttgaaatc gagcacccgt gatgagataa cctcaccatc ggggagcatt aactcaagga
    81001 aagcaggttg ctcattgaac ggccagaaac gtcctttgag gttgatggtg ccgccgaaaa
    81061 tttcatcacc atcttcaaga ccttcgtaga tgatgcgttc gtaaatcatg ttgcctaacg
    81121 ggttgacctg attgacgccg acggaatata aaagcacggg catggtgttg agcgcttgaa
    81181 ttcgtccgtg gtcatctttg gagctgatgc ggatatatcc tttttcagaa acggcgcgga
    81241 tctcaaagga aagctcaaag ctgcgatagt tgcctgccag gtttctctgc acgcgttcca
    81301 gcacccgctc aaggacacgg ccatcttcac cgagcaggtc cacttgtacg atctcgcttt
    81361 cgcctgcaat gaggatgacc tgcagattga tcggagatac aaggctcgac attggccccg
    81421 gcgacaggaa gtgaatttcg gcgaagtcgt taaaccccgc ggcgacagtt ggtgtggcgg
    81481 aggggagtgg ggtagtgggg acaggtgttt ctgttggaac gagcgtttcg gtcggcgggg
    81541 cggaggtggg gaccaaggca ctggctgtgg cgaaggcggc ctgtcctgtc aacgcgacaa
    81601 cggtgggcag ggagtgagcg ggagtctgtg tgggggctgg gcctgttaat tgtgtgcagg
    81661 cagttatcag cgtgagaaga gaggcgagaa gcagggggtg ttttttcatg ggcgcaggaa
    81721 tcaatatccc gcaggcagtg atggagtttc gattcgttct ccagattgcc tgcgggatgt
    81781 gttttttaca ggccgtaaat atcgctgtac ttggtattca ggtagcgtac gtaggcggct
    81841 gaatcgatcc tggagccagt gaccttttga acgatatcct gcgggtcgta tttgtggccg
    81901 aaggtgtgaa tcttttcttg cagccagtgg agcagggtat cgaatttgcc cttgcggatc
    81961 tgatcgtcga ggtctttgat gtctttgttg atcttttccc aaagctgggc agagacgagg
    82021 ttgccaagcg cgtaggtgga gaaatatccc atcaggccat tggaccagtg gacatcttgc
    82081 agaacgccca gggcattgtt tggcggaaca attccaagat attcctgcat ctttgtgttc
    82141 cagatttcgg gcagatcttt gactgcgaaa cttccatcca ccatgccgac ttcgagctcg
    82201 aggcggagca tgacatgcag gttgtaggtg gcttcatcgg cattgacgcg gatgaaagag
    82261 gattccactt tgttgatgcc tttgtagaag gctttgaggc tgacgccgtc caactgggag
    82321 gggaaggctt ttttcaggtc tgggtagaaa tgttcccaga atgggagcga acgcccgaca
    82381 aggttttccc acaggcgcga ttgggattcg tgtacggcca gtgaagtgcc gcctgcaagg
    82441 ggtgtgcgtt cgtaggcggg gttgctgccg agttcgtaaa aagcgtgacc tgcttcatgc
    82501 atggcgctga agagggtggc catcgggttg ttgggttcgt agcggttggt gatgcgggca
    82561 tcgttgacgc tgaatgtggt ttggaagggg tggggggctt tatcctgcct gccgcgattc
    82621 cagtcgtagc cgaacttggt gatgatagct tcgctgaagg cccaaacctt cttttcgttg
    82681 tacttctttt gcaggaaatc gtctttgacc tgtttggaag acttgatggc ttttagcaat
    82741 ttcacctgct gcgggcgtag tttttcgaaa atgccctgta cttcggcggt tttcatgccg
    82801 ggttcgtagt catcaagcag ggtatcgtag gggtgatctg caggcgggaa gaaggagaca
    82861 tattcacgca ccagttcgag cacgcgttca aggtgcggtt ggaagagggc gaagtcagat
    82921 ttgcctttgg cttcaaccca ggcatcaaag gctttggagg tggtgagagc ctgcctggca
    82981 acgaaggagg cgggtacccg tgtggattta tcgtagttgc gtgaagccac acggatgagg
    83041 gcgccatcat cggtgttggg gtcggtgtat tcctttttga gttcatcaag cagcttgccg
    83101 acttcgtcgg aggtggattt ttcgtgggcg atcttgccca atgtcgagag ttgctgtcca
    83161 cgggcttcgc tgccgccagg gggcatgctc acattttggt cccattcaag caccgatgcg
    83221 gcatggtata ggtcgttgac ttctccgaga atttccttta actgatttaa cttttcagac
    83281 acaaaaacct ccagattggg taaattgagc gactttcgtc aaattgatga atttcagtga
    83341 tcaattactg ctttctttga ttttatccca tgcggggtga ttttacatcc tccctgcgaa
    83401 ggggttcatt gaaatttgcg gataaaaaag ccacccgtga aagggtggct tttgccagtt
    83461 cgggttatgg gcatttgcgg tgctcgcctg caccgtcgtc taaaatttcc acggtgacgc
    83521 cttcgcgtac ttccacagtg gtgacttcgg gttcaccagc cacatccatg tcatagacgg
    83581 tgtaggtgcc ggggttgagc cagaccatgt tatcggggtg atcaagcgag gagagcaaaa
    83641 tgacctcggc aggcaggatg tacatttgat ggtgggtgca gtttcgcaga taggcaggga
    83701 gaggccaatc ggtgggggcg agttcgggca gggttcggag gtcgccatcc acttgagagt
    83761 attgggtttg cagggagacc cagccttttt ggtcagagtc ggggatgacg atctgtaccc
    83821 agttggagag aacgtcacgt gcaagcacgg gggtgcttgt atctttataa agcacaccga
    83881 ttggattgaa ggccatatcc gggccgcgac ggatgaagag attccctttg accgcgtgaa
    83941 tgacgacctc gggctggggt gttgcagaag ggagagtctc tgtcgctgtg gcaggggcag
    84001 ttgtcggggt ggtggaggcg gtggctgtgg cggctggcga ttcaaccgcc accgtttcaa
    84061 tatcctgcgg ggtgggcagt gcgaggggag catccactct aacttgcata catgcctgaa
    84121 gcaagaccac gatgagagcg gcggtgagga tgcttcgttt gatttgaaaa gttttggtca
    84181 ttcacttgcc tattcgtagc gcagggcttc cacaggttcg aggttggcgg cgcggctggc
    84241 ggggtagatt ccgaagaaca ggccgatgat ggcggaaaaa ctggtggaga gcaaaatggc
    84301 atcggtgccg acgatgggga cgaagttgtt acctgtagca gtggcgacct gccccactgc
    84361 gaacgcgatc aaccagccga agatgactcc gataacgccg ccgatcaaac ttagcaggga
    84421 cgattcggtc aggaattgca gaaggatgtc ttttttgcgc gcgcccagtg ctttgcgcag
    84481 gccgatctcg cgggtgcgct ctgtaacgga aaccagcatg atgttcatga tgccgatgcc
    84541 gcccacaagc agggagatgc ctgcaatacc gccaaggaag atggtgagta cgccggtgat
    84601 ggtttcgaag gtctgtagaa aatcttgctg ggtgaatacg gtgaaatcgt catcgccgat
    84661 gggggtgcgg tgacgctggc gcaggatgtt cgaaatctca tctgcggcct gtggtactga
    84721 ttcggcggtg gtggcttgta caaaaagtac gtcaatctca tcgagagaag tgcgtttgat
    84781 aaggcgcgct tgtgcggtgg tgaatggaat gtaggcgctg ttatcttcac tgccaaatgc
    84841 gccgccgcct ttggcaacca gcacaccaat gacgcggaac ggctggcctt cgatgcggat
    84901 cgtttcaccg acgatgccat cggcgtggcc aaagatggca ttggctgttt ctggcccaag
    84961 gactaccacc gacattcttc cgaggatgtg ttcttcgttg atgaactcac cttcggcgat
    85021 ctcaaggttg cgcaccggtg catagtttgg tgtgacacct gagatggtgg tggtggtatt
    85081 ttcgccagca aaggtaatgg ttccattgcc ttgaatggcg ggagctacaa tttccaccga
    85141 tggtgcggcg agcggatctg caatggcttc tgcatctgaa agggtgaggg gacggtcatt
    85201 atttccgctt ccgcgaccgc cgccaccggg tccaggcgga ccatcgggac tgcccttgaa
    85261 tacgaacaac aggttcgtgc cgatgctgct gatggaacct gtgatggatg cctgtgcgcc
    85321 attgccaact gcaagcatgg cgatcaccgc tgccacgcca atgacaatgc cgagcacggt
    85381 caggccggag cgcattttat ttccgttcaa actttcgatg gcttcgagaa gggcttgtga
    85441 aaagctcata atctcatccc ttccttttta tttcagatgc agggtccaat tcgccatcac
    85501 gcagacgaat gacacgctgg gtttgctctg cgatggtggg gtcatgggta acgatgatga
    85561 gggtggtgcc gcggtctttg ttgagattga gcaaaaggct catgatttcc ttgccaacct
    85621 ttgaatccag gttgcctgtg ggttcatcgg ccataataat ggcagggttg ttgaccaggg
    85681 aacgcgccac agccacacgt tgttgctgac cgccggagag ttcatagggg cggtgcagca
    85741 tacggtcttt caggcccacc gcttcaaggg cggcctgtgc tcgttcgcgg cggccttcgg
    85801 tgatgccggc atagcgcagg ggcagttcca catttccaag tgcggtaagc cgtgagagca
    85861 ggttgaaact ctgaaagacg aatccgacct tgcggttgcg cacactggca agctggtcat
    85921 cgttcatttc agcgaccgat tcaccatcca gaatgtaatc gccggaggtg ggcctatcca
    85981 ggcagcccag cgtattcatc atggtggatt tgcccgaacc ggaaggtccc ataatggaaa
    86041 ccacttcgcc gcgttcgatg gtgaatgaga cgccgcgcaa ggcttccacg atgatctcgc
    86101 ccatctgata tgtttttcga agatcgcgag cttcgatcac cagatccata ttagcctcca
    86161 aacggcccgc cattattgga tggtggattc aaaataatga gatcgccttc cttgatatcg
    86221 ccttcggcta aaacgctcat tgtgtcggat gaagaaccga gcttgatctt gacctgaaca
    86281 ggctgcccgt caacgagcag atacaccacc cgttcaccat ccaccaatct cactgcacgg
    86341 ttggggatga gcagcatgtc tttttgttct tcgatcacca cattcacagc ggcggtcatg
    86401 cctggtttta caagttcatc ggcatcggtc aactcgaccg tcactgtaaa actgaccaca
    86461 ccctgcaccg tgtcaccagc ctgacccact tcaaccactt caccgtgata ttccttgcca
    86521 agaatggcat caaaggtcaa tacggcgggt tggccgatgg ctacgttgtt gatatccact
    86581 tcagagactt gcaaatcaac cagcatgtgg gagacatcgt caatgcggaa ggcgaccgtg
    86641 ccagcagtta cctgatctcc aacagaaggt tctgattggg tgatgacccc atcaaatggg
    86701 gcaatgatat gcgccatgtt catggtggat tgtgctgctg ccacgcgtcc ttctgctgcg
    86761 gctagttcag cggggtccac accattttgc agacggtcca aattgcggcg ggcgtcgttt
    86821 aattgagcct ctgccagggc gagcttttca tcagcgtcgg cgatggtttc ggcatcagca
    86881 taacctttgt acgtgcggac tttgggaacg ccgcgtacaa gataggtttc agtgatggta
    86941 ataagaccgt tcagttccag gcggtaatca taggcttttt tataggcttc ttccgcggca
    87001 tctactgtgc gcaaagcctg ggcgcgggct gtatctgaat tgagcaggtc tgccagtgcc
    87061 ttttcggccg cagccagatc tgactctgcg aggatgacac tctggttgag agaattttta
    87121 tcgaggctgg ccagtatctc gtcacgtttg accgggtttc caacttccat ataaatatct
    87181 ttgacgatgc cagtcgtctg ccagatgagt acagcgcttt gtcttgcgcg aacgctaccc
    87241 gttgccccga ctgtggcaac cagattgcca cgttcaacgg tggtggtttg gtaaagaggt
    87301 tctccgctgc ttcttgtgct aaaaaatact attgcaagca cgaccaccgc cagaacgccg
    87361 atgatgaccc acgttctgcg ggtgatcttt gaaaaaaatt gtttcattgt gatttatcct
    87421 ccaggaagac agtcaatgtg cttccttgta ttcttacttc atttatcttg taagtttaac
    87481 gaatgaatgt gtagaaattg tgcaggtttg gtttctttct attctaattt aggaatacga
    87541 aaaggagctc ttttcggtac gggaattaag aaatgtctaa tggtaaaatt gtgatgattg
    87601 tcatcgtgtt tggtgacata taagcctgtg tctgtacaag tagatttagt atcatcatgt
    87661 gtaggttgca agcaatttta tatgtagtgg aaaccttttt tttgtttctg gaggtattat
    87721 gcaggctgtt cccagcgaat ttttggccat tgatcgaaaa gagcgtgcca atcgcccctt
    87781 tttcgacctt gaccttcgcc cggcagaggt tcgaacttgt gattttgatg atgtagtcat
    87841 tgaatttgac cccgaacgcg cgatggttga agccgcgcgt tgtattcatt gccctgatcc
    87901 tgctccctgc atggtagcct gcccaaccgc caatgacatt ccctcggcca tgtggttgat
    87961 cgaacaggga cgttttgtgg aagctgcggg gatctatcat aaaaccagtt ccctgcccga
    88021 agtgtgcgga cgtgtatgtc cgcatgaagc tttgtgtcag ggttcatgcg tcttaaacaa
    88081 acatcaccaa cctgtgctgt gcggagaatt ggaagccttt accgtagatt atgaacgccg
    88141 cacacagggt tatgtcatcc cgctcgggat tccctcagga aaaaaggtgg ctgtgatcgg
    88201 tgctggccct gctggtttgg gctgtgccga agtgctcgtt caaaaagggc acgatgtgac
    88261 catctttgaa tccaaacctg cgccgggtgg tttgcttgtg tatggcattc cgaatttcaa
    88321 actctccaag gaagtatggc aggaaaaatg gggagagttt gaacgcgctg gtgtgaaatt
    88381 cgttcccaat acctacatcg gtaaagataa gaccattgac ggcctttttg aagaaggctt
    88441 tgaagctgtt ttcattggcg tcggctctga agtggacgca aaaatggaag acaccccagg
    88501 caccgaactg cccggcgtgt acgaagcaac agatttcctg atccgcggta atgtggagcg
    88561 cgatcaactc cctgagcaca tgcgtgcgcc gctggaagtt ggcaagcgtg ttgtggtcat
    88621 cggcggcggt gacactgcct ccgactgtct gcgcaccgcc atcagactcg gctcaaaaga
    88681 tgtaacctgt ctctatcgcc gtaccgaaaa agaaatgccc ggtggcaaga aagaccgcaa
    88741 gatggcccgc gaggaaggcg caaaatatcg cttccttacc cagcctgtaa aattcattgc
    88801 aggttcagat ggcaagcttg ccgctgtgga atgcatcgaa atgaaactcg gtgaaccaga
    88861 tgccaaaggt cgccgcaaac cggtcccggt ggaaggctcc aatttcagcg tggctgccga
    88921 tactgccatt cttgcgttgg gctactggcc tgatcccatc attggaaaga ccacccccga
    88981 actcgaagtt catgattggg gtttgatcac cgtgaaagac aaagccaggg gcaacacatc
    89041 acgccccggc gtattctctg gcggcgactg tgtcacaggc ccggacctcg tcgtcaccgc
    89101 aatggttggc ggcagaaaag ccgcactggc cattgatgaa tacctcaaaa ataaaaaata
    89161 acctgttgtg agggcagggt ataaagcagg gcgggacgca agtcccgccc tgcgcttttg
    89221 ttacgtcctg cccgtacggg tataatccaa cccatgtttc aacgcatcat acgcgggctt
    89281 gagatcaccc tgatcttgtt catcattgcc gcaattaccg gacacggcaa cccatccctt
    89341 acagataaga tcgaaaaggt acgcgcattt acacgccaga tcgaattcaa ttacatggca
    89401 tggatgggca atgctgccct gctcaaattg cgctccgcat cggttggggt gccgtacaca
    89461 ctcaactacg aatcacgcaa gcagatcgtc actgaatatt tgcgtgtcac acaatccatc
    89521 ctcgaaaacg aatatcagct tcagcagatc tatgccgatg ccgccattac tgacaaggaa
    89581 ctcgcctcgg aagaattgcg cgccgaactt gatgaacaat acgcgcggca ggccaaactt
    89641 gccccattgg ctgaagccac cctgcaggaa caggtaacca tcatcctctc ggaaattggc
    89701 cttgctgctg ttgggcagcc tgtgccgaat gtttggtatc actccacacc gctgccatat
    89761 gcgctcattg tttccccgcg tgaccgcatt gaacagatcg caaatatttc catcaatact
    89821 gaaatcactc ttgatgagca ggccgccctt gaaagtcgtg tggatgcagg cctcaacacc
    89881 tcatcgttgg tggtgccgat cggcggggtg ggggtatacc caaccatggt catgcgcagt
    89941 acgaacctgc cgtgggtgct caataccatt tcacacgaat ggattcacaa ctacctgacc
    90001 ctgcgtccgc ttggtttttt gtatgccaac acacccgagt tgcgcaccat gaatgagaca
    90061 accgcttcca tcgccgggaa cgagatcgga gcgcttgtca tcgaacgctt ttaccctgaa
    90121 ttaaaacccg cttcgcgctt aagcccgggc ctgatctctg cgccatttga ccaccccgaa
    90181 cccggtgact ttttgcgccc cccctttgat ttccgcgcgg aaatgcacga aacgcgcatc
    90241 acagtggatg ccatgcttgc cgaaggtcag atcgaagaag cggaagccta tatggaagcg
    90301 cgccgcctcc tttttctgca aaatggatac ttgcttcgta agatcaatca ggcttatttt
    90361 gcattttacg gtgcctatgc cgattcaccc ggtggcgcgg ctggcgaaga ccccgttggc
    90421 cctgctgtgc gtgccctgcg tgagcaaagc gaatctttgg ccgattttgt caacaccatt
    90481 tcgtggatga cgaatttcga gcaattgcag gaagcaatca aaaagtaaca tggcgggaga
    90541 gagtcaattc ccatttgcag attttgccaa tgcagcggtc tgtacggcaa aagcgggaaa
    90601 gtctgaaatc aaaatcagga aaccatgaaa cgaatcgtat tttttttgat aatctccatt
    90661 gtaagtatga tcggtctaag tgcgtgtgcc gctgaagccg agactgcccc ggttactgtg
    90721 gcggttcagc cgaccagcct gcccgatgtg gccacgccca tttccgaatc aagttgctct
    90781 gcgatcgttg ttgcgccgac cccaacccca ggtgtggatt cgtacttccc gccagccact
    90841 gcggatgatt tttcctacgg ctctgccgat gccccagtga ccatcattga gtattgcgat
    90901 tttcaatcac cgggctgtgg cgcgatggcg ctggttttcg cagaattttt gcgcagtcag
    90961 gaaaatgtcc gctttgtgtt tcggccagtg ccattattgg atgtgatgga taaatcagac
    91021 aaggccacca tggctgcgat tgcggcgggg gaacagggtc aattttggaa tatgtacgac
    91081 ctgctctatt tgcgtcaaca ggaatggata aacatggagc cgcgcgcgtt cgatgattgg
    91141 gtggtgcggg aagctgtcat tttggggttg gatggagaaa aactcacagc ggcgatgaat
    91201 gctcctcaaa cagaagccag catgaggtcc atgtatgagg cggcgaagaa gttgaggatc
    91261 cccgctgtgc cgctggtcct catcaataat tcgccccagt cagccaacat cctcaattat
    91321 cgaaacttaa gcgagacggt tgggctgatc gcgcttgggc agaagcagtt tagcgagtgt
    91381 ccgccttttg atattgatcc cgtgaagcaa tacattgcca cggtgaagac cgagaagggt
    91441 gacattgtga tacagttgtt tccggataaa gccccgctgg cggtgaattc gtttgtgttc
    91501 ctggcccggc agggatggtt cagcggcgtt acctttcatc gggtattgcc tggtttcgtg
    91561 gcacaagcag gtgacccaag cggtacaggc aggggcaacc ccggctattt tttcaataat
    91621 gaattcagcg atctgaaatt tgataaaccc ggtgtggtgg gcatggccaa ttcagggttg
    91681 aatatgaacg gaagccagtt ctttattacg tttggccccg cttctcacct tgatggcggt
    91741 tacaccattt ttggccaggt tatcagcgga ttggacgtgg ccgagcaatt gacaccacgt
    91801 gaccccgatc aggcggtcat ccctgcgccc ggcgacaaga tcatcagtgt ggagattgaa
    91861 gaaaaataat gcagacaaaa aatacccatt ggccttcgat attaattctg atcgtacttg
    91921 gtacaagtgt gctgtttgtg tttttgttca tcgccgctac gggaatcagc tcgatcattg
    91981 acctgttttc agaaagcggg gaccctgccg gggaaatgat cagcacattt gcatttgggt
    92041 ttgaattgct ggtcttgctg gtgtgcatgt ggttcgtgtt ccagaaagcg cagagccgcg
    92101 aacaagccga tctccaattc aaattcccat ttgccaattg gcagatcttg gccgtgctgg
    92161 gtttgatagg cctttcggtg atgctgggtg cggcagttat ttatactgaa gtgattgcat
    92221 tggcctgggt ggtgcttccg ttcttgacct tgcttgtgat cgtgccgccg atctggattt
    92281 tctttgggtt gggtacaaag ggaattgact ttggttcacg ctggagggtt ttcagtattt
    92341 tgggccttgg catgaccctt gcaccggtca ccatgatcgt gttggaactt atcattttat
    92401 ttgtgatcat catcgcgggc accgtatttc tggcactcag ccagcccgat ctgctgcaag
    92461 aggttgcggc tgttggcaga attctgcaga atgaaacaga ccaggatgtg attctgaatc
    92521 tggtggctcc ctacctttcc agcccggcgg tgatcgcaac cctgatcggg tacatcggtt
    92581 ttattgtgcc gctgatcgag gaactgttga aaccattggg agtgtggctc tttgcccgga
    92641 aattcacttc gccagcccag ggatttgcaa tggggatgtt gagcggtggt gcatttgccc
    92701 tggtggaaag cctcaatgca agcgggaatg gttcagccga atgggttgtg gttgtgagcg
    92761 tgcgtgctgg gacgagttta ctgcacatgg ttgcctcggg tttggtgggg tggggcatcg
    92821 tttcggcgtt tcaagagcgg cgctatttac ggttgccagc cgcgtatttt tgtgccgttg
    92881 ccattcatgg gttgtggaat tcctgtgcga tcggcatggg catttcttca gtgggcgagt
    92941 cgcttggcag gccggaatgg ctggccgcgt atgccccgtc catgctgggc gggttggtag
    93001 tgcttggggt ggggatgttt gttgtgctgc tggcagcgaa cagaaagtta agcagtgtag
    93061 ccgtcattag cccgacccct cctgccgaaa ccagggcaga gacggaagaa aaggtaaaat
    93121 aactgcatga ttgattcgac aattacacac gataatgacc tgatccaatt ggtggggctg
    93181 acacacaaac attttatttt ttcgctcaag gcaggcgctg aacttcatac gcaccgcggc
    93241 atgatcaaac atgatgactt gatcggcaag ccctggggtt cgcaggtatt tagccatact
    93301 ggcgcgccgt tttttctgtt gcagccatcc attgcagata ttctcaatga cctgccgcgt
    93361 gccacccaaa ttctgtatcc aaaagatatc ggctacatcc tgattcaaat gggtgttgcg
    93421 cccggtcagc gggtgatgga ggctggtact ggctcgggct cgatgaccat cgcgctagcc
    93481 acggctgttg gtgcgaatgg ccgtgtcatt tcctatgaac aaaaacagga tacgcaaacc
    93541 ctggcgaaga aaaatctcga acgggtcggc cttgcctccc gtgtggaatt taagttgcgt
    93601 aatattcagg aaggcttcga cgagaccgga gtggatgctt tttttctgga tgtgcaaaac
    93661 ccatacgatt acattccaca ggttcgtgcg gcgcttaagc ctggcggctt cttctgtact
    93721 ttaattccca ccttcaatca ggttgaaaag accctgatcg cgttgcgcaa aaatcaattt
    93781 gcatttgtgg aagtctgtga attaatgctg cgtcattaca aagcagattc agcccgcatc
    93841 cgcccgaccg accgcatggt tgcccatact gggtttttga tcttcgggag aaggatcgaa
    93901 cccaataccg atccgcgcgg gaaggaactc gaacaagaga tcggcatgga agaatagcca
    93961 tttcgatcct gctcagttga atttattgtt tgaaacgaac ggaggttcat gatgtttcgt
    94021 cgaagaggtc aacaacgaat gtttcgcggc ggcaggcgtg aagggccgcc tgttttgcag
    94081 caggccaatc aactcatgct ggatggcgaa tatgaagagg cttcaagcct tttcgcagat
    94141 cttggccagc gcgccgagga acgttttcca catcgcgcgc cgtttctttt cattgaagcg
    94201 gggcgggccg ccattttggg cgggcagacc aaaacaggtg ttgcccacct tcgccgtggc
    94261 ttgaccctct tcgcatcaca ggggcgggtg catcggatgc gtatggtggg tgaacgcgcc
    94321 attgatgaat tgcaggcccg cggattgaaa gcggaagcgg aggaaatagc ggccctgctc
    94381 ggttcaaact cactgccaga tgcttcgggt gaaaaggaaa ttcccgcccc gacgaagcgg
    94441 cctgtcctgc ccacgcattg tccatcttgt ggtgcagccg tgcgccccga tgaagtggaa
    94501 tggcttgacg aggtcactgc cgagtgtgcg tattgcggca gcccggtacg gtaaacccag
    94561 ccatgaatct gaccgaagaa tacatcacgc aaaaacttca ggcggccatg cgatcttcca
    94621 cagatgccga tggatacgcg gaaatggcgg ttcacccgga cacacgcttg aagtgtgcgg
    94681 cagtgctggt gccgctgacg caagtcaacg gggaatggca tttgttgttc acgcgccgca
    94741 ctgaaattgt gcaatcacac aagggacagg tttccttccc cggcggcgcc tgcgacgagg
    94801 gagagaccac gcccgaacaa accgccctgc gcgaagccga ggaggaaatc gggctgcggc
    94861 ctgccgatgt cagagtcatc gggagactca gcaacttgat caccatcagc agctttcggg
    94921 tgagtccgat cgtgggggtc atcccgtggc cgtacacgtt taaagtggcg ggagttgaag
    94981 tggcgcgggt gtttacaatt cccctgttgt ggctttcaga ccgaaataat tactgggagt
    95041 ttttcgtgcg cgattcagaa cggtcactga tcgcatatca tccttatgat ggcgagttgc
    95101 tgtggggtgc caccgcaagg atgaccgtga cttttttgaa gaccatcgaa acgcttgcaa
    95161 gaaaataagt tacgagtttt tttcgaaggt gccatcaaga tttaaataaa acgtatccac
    95221 ttcaaggtgg gggaagagcc gccgaatttt atcttctgct ttatcaaggt cttcttcgtg
    95281 acgttcctcg gtgccgccgg gaccgtacat gccgcaatct tcatggttaa tgagaatgac
    95341 ctttgagatt ccatgcaggc gcgcggcgat atccacctga cggatgacat cgtacacatc
    95401 atacacaccg cctgccatta cggcgcggtc ataagtgcct ttttcaaaat tggattcgag
    95461 ccattgattg atatgggatt gcaaacggta gtccatgcaa tgaacgacga tggcctggca
    95521 tttgtgggtc atgggggttt ccttttatga aatcggttcg aatgtcccat ccagatgcag
    95581 gtagaaggtt tcaacatgca aatgggggaa gagtttttta atcttggcct gcgcatcttt
    95641 caggtcatgt ttgtggcgtt tgtaggtgcc atcgcgcccg taggcacggc aatcttcgtg
    95701 attgatcaaa cagaccgttt caatactgtg gatgagtacc gcaagctgta cgtatttcaa
    95761 gaccatttcg taatcatgta cgctgcctgc cagcgaaatg atgtcaaagt tatcgtaccc
    95821 aaaacggacc gtgatccacg gttggatgaa tttttgcagg cgatagtcca tgcagtggac
    95881 aacaagcgca tcgcaatgaa tgatcggttt tttagctggc atgatttgat ttacctgtaa
    95941 agttagttgt tgaggggtat cttttataaa atttcgcctg cgccgcgttt caagaactga
    96001 ccgtcgccgc gtttgccttt ccattcttcg ccatccacga tcaactttcc gcgcaggaaa
    96061 actttttcag ggtaccctgc aagttcccag ccttcataca aattatagtc agtgcgttga
    96121 tgtgaatgtg ccactccata tttgacctgt ttctcaggat cccagatcac aatatctgca
    96181 tctgcacctg ggatgagcgc tcccttgcgc ggatacaggc cgaagatctt ggcagggttt
    96241 gtgcagttgt atgccacaaa ttgattgggc gtgatatagc ctgcacgcac accataagtc
    96301 cacatgatgg gcatgcggtc ttgaatgcct ggcaggccgt tggggatctt tgtgaaatcg
    96361 tctttgccca gttcttttcc ggggatggcg atctcgcttc cttcatagac gatgggactg
    96421 gtaccgtcaa aataaaaggg gcaatggtct gtgccgaccg tttgcaaaac cccgcctgaa
    96481 agaccttccc ataaacgggc attatcctgt tcggtacgca ttggaggaga gcagatccat
    96541 ttggcaccat cggggcggta cagatggtca atggtgaaga ataaatattg cggacaggtt
    96601 tcgcccatta ccttgacacc gttttcacgg gcgtatttga gcatatccac ttcacctccc
    96661 gcattcatgt ggacgatgta aacaggggaa tcggcttgct ctgccatggc ggccatgcgc
    96721 aaggtcgctt ccacggctcc ccaggcaggg cgggtgtatg catgccactc aggggtggtg
    96781 tgcccagcgt tgagcgcttc tgggatgagc gcttcgatga catctccgtt ttcgcaatgg
    96841 gccatcatca acatgccgtt ttctttggca atgcgcaggg ctttgaagat actgccgtca
    96901 tcaatgcgta gacggccatt gtaggcggtg aagaccttga gcgtttgaat gcccatcttc
    96961 atcagcgatg gaatctcttt ggcgatcttg tcgttgaaat tcgtcagatt catgtggaag
    97021 gaataatcaa tggcggcttt ttccgctttc ttcatccaca ggtccacgga atggtcaaag
    97081 ccttcatttt ccaggacgac aaaatccatt acggtggtgg tgccgccaaa cgcagccgcc
    97141 ttgtgaccgg tgtaatggtc atcggatgag acggtgccaa acatgggcag gtcgagatgt
    97201 acatgggggt cgattccacc gggcatgatg agcctgtccg tagcgtcgat cacttcagca
    97261 ttcgggtgat gtaaatccct gccgatctgg gcgatggtct cgccttcgat cagaatgtct
    97321 gctgcgaatg tatcagaggc ggtgacgaga gtgccgtttt tgatgagggt gttcatgttt
    97381 tactccaatt gtgttcagag ttgtcaaatg tgccagaaga gccaaaaagc tcctgctttg
    97441 ggagagcttc agccaatttg taaaccatca aaaccagttg atggcttttt gccagacaat
    97501 caactttggg aatcctttcc ctatcttatc tgtctttttc attttattca ttttcttttt
    97561 gcgcatttgg tgcgcctgcc tcatatggct cttgcaattc cttgatcccc agcagcgcca
    97621 acagcacctc cgggggaata tctgcctcgg gaagatcggt tttgtataac tgctcattct
    97681 cggcacgttc gcgcaggttt ttccagagca gttgacgttc cccgccttcc accaccaggt
    97741 cgttcaaagt ctttgcgttt aagatgtaat cgcctgtgga atacaaaaat gtgaggcgtt
    97801 tccatttgtc ggttttgatg ggttctttta atttttcgag tccgcccagt tgaattttgt
    97861 agtattcctc tttcgcgcgc ggatggttgc gttcatcttt taggagttca ccgcgtgtgg
    97921 tcagttcgtg gcccttcacc tgggcgatgc attcgatttg tccgctgcgc tcgccgaatg
    97981 ctgcgggttg ataaaaggcc agataatcca cggcaacaac tttcggcgcg gtgcgaagcg
    98041 gaatgcgata ccatcccaac aagcgggcga tctccatatc ccgtggggtg ggaatgagag
    98101 cgaccagaac caagtctgta gctttcagca tggattaatt ataagtcact tgcaaatcgg
    98161 gcggcagata aataaaaatc ctgccagtct ggcaggattt tttgtgatct agatggatgg
    98221 cattcgtgtg tgccattctg ttgcttgttg aaatgcgtag cctgtgcgga ggacgcccgc
    98281 ttcattccat gcccgtgaga cgatctgcaa tccgatgggc agaccttcac ttgtaaaccc
    98341 gcatgggatg gtgagtgcgg gcaggccggt caggttgaag ggggctgtaa aacgggtgag
    98401 ttgacgggca cgttcgagtg cgttttcgcc ttcgagtacg ggggccgtga tgggagtggt
    98461 ggggagcagg aggccatcat attcttcgaa aagcaattca agctggcgtt tgacttcagc
    98521 ctgtgtgcgc cttgccagaa tatattcgct ggaggtgaat gcagctccga tttccaatct
    98581 ttggcgtaca tcggcgccaa accaatcggg atgttccttc aaacgttcgc ggtgaaaagc
    98641 ggcaccatcg gcttgggtca tcagggcgtt ggcaacggcg gcttctttga ggaagtcgac
    98701 attggtttcg gtgatgaccg cgccttgttc ttccaatact tgtgcggttt tgcgaactgc
    98761 ttccagaact tcggggtctg actcttcaat aaaactgcct gttgccagtg cgaatttgcg
    98821 gtctttgatt ccatcgcgca ggtggctgta ataatcgcct ggcagggtct tcatggaggc
    98881 ggggtcaagc tcatcgtatc cgcccatcac ttgcagcatg agagcggcat cttctacttt
    98941 gcgtgtgatc gggcctgcat gatcaagatt ccatgaaagc ggcagaatac cgcgcaagct
    99001 gacccgccca aaggtgggtt tgagtccgac cactccgcac aatgaggcag ggatgcggat
    99061 tgagccgccg gtgtcggttc caagtgcagc catggccatg cctgtggaaa cggctaccgc
    99121 actgccgcca gaggaaccac cgggggtacg tgtgatatcc catgggtttc tgcatgcacc
    99181 ataatgcgga ttgttgttgg tgaccccgag cgcgatctca tgggtgttgg tctttccaat
    99241 gatctgcgcg cctgcctttt ttattttttg cacaacgtag gcatcttcct gcggaatgtg
    99301 atcggcaaaa aaacgtgtcc ctgctgtagt gcggattccc tttgtgttgt acaggtcttt
    99361 cacggcaatg ggaatctctt gcagaaggcc gctttgcgtt tcttcctgcg cggagagttg
    99421 ctcgggcgga ataaccgtga tgaacgcatt tagcgtcggg ttgagccgct cgatttgacg
    99481 atagcaggca tcggcgagtt cggaggcgtt gatttccctg ctttttatga ggtcacgctg
    99541 atcagtgagg gacaggaatg aaaggttcat gtaaggtcaa ttataaccaa acaggacaat
    99601 actcccatag cctatctgta ctgatatctt acaatgataa ggtcttaata tcaatgactc
    99661 agaaggagta aaaaatgaaa aagacaaaaa ctattttgct ggcaatgacc gtcctgcttg
    99721 ccctgtgggt ttccgcctgc ggtggctcgg ctgccccggc cacccaggcc gctgtgcccc
    99781 ctgttgtaga aagtgcgccg actcaggcgc ccgttgttga atctgcacct gtacaagaac
    99841 aaccacttgc gaccgttcaa tcatttgctc cggcctgtca ggctgctgct tcatgcgccg
    99901 cccccgccgt tacggataca gttgcatcag atcgctactg cgttgagaag attccgtatc
    99961 agaatattct tgttgacgat ggcgtgactt tcgaagtgct tgatcctgcg aacttgatct
   100021 gttccgataa tggcaccatg gtggatggca agcatgtagt ggaatgtcat ggcactgagg
   100081 cgtggtccag ccaggtgaaa ttctccaaca cggcttgtag cgctgctggt ttgcaaactg
   100141 gcacaggcca atgtcaggaa gggttgggct atgatgccgc gaataattgc tgcgcgcccc
   100201 tttctgctgg tgatgctggt tcgatcacca ttacggtcaa catgggtgag tgccccaact
   100261 aataagtttc aaagtttttc ataaaatata aagaaaacgg ccctcgaaga gaactcttcg
   100321 ggggccgttg gtattaaagt aaatctgact agaacagacc aagcaccttg cctgtttcga
   100381 ggtcaaggtc tacatcgtag aaggaggggc gggtgggcag gccaggcatg gtgcgcatat
   100441 cgccaaggat gggataaagg aagccagcgc caaccgaagc gcggatctcg cgtacggtga
   100501 tgcggaagcc tgtgggggcg cctttcttgg tggcatcggt gctgaaggag agatgcgtct
   100561 ttgccatgca gatcggcagt ttatcgaagc cgaggcgggt gtagcgttcg atctgttctt
   100621 cggcttcggg ggtgtaatcc acaccatcgg cgcggtagat cttggaggca atgtcttcga
   100681 tcttgctctt gatggggttt tccagcggat acaggaattg gaagttgctg ggtttggagg
   100741 cagcctttac aacggcttcg gccaatgcct tcgcgccagc gccgccatcg gcccagtgag
   100801 tggagacgac ggcatccatc gcgcccatct tcaacgcagc ttcgcggatc atttccactt
   100861 cggctttggt atcggtggca aacgaattga ccgccacgac cacattcaca ccgaacttaa
   100921 gcgcgttctg aatgtgacgt tcgaggttgg gcagacctgc tttgagcagt tcgaggtttt
   100981 catcggtgta ttctggggca agcggtttgc cagccacaac cttcgggccg ccgccatgca
   101041 tcttcagtgc gcggacggtg gctaccatca ccaccacctg cggaatcaaa cctgagtagc
   101101 ggcatttgat gttgaagaat ttttccatgc cgatatcagc gccgaaacca gattcggtga
   101161 ccacgaagcc atccttgccg accagcttga gtgcgatcat atctgcaatg atggaggatt
   101221 gtccgtgggc gatattggca aacgggccag catgaacagt ggcgggagtg ccttcgaggg
   101281 tttgcatcag gttgggtttg atggcgtctt tcatcaatac ggtcacagcg ccagccacac
   101341 cgaggtcttc cacggtcaca gcttcgccgc gtttgttggt tgctacaacc attctgccga
   101401 agcggtcgcg catatcttcg aggccagtgg tcaacgccag cactgccatg atctcagatg
   101461 ccacggtgat atcgtagcca gacatgtgcg catggccagc ctcatccttg ccgcggccta
   101521 cttcaatttc acgcagcata cggtcattga tgtccactac gcggcgccag gtgatggtct
   101581 tctcatcaat atcgaggcgg gcgaagcgcg aacgttcttc aggggtcaag tcgttgggat
   101641 tggtcttctt gatgccaagc ttcgccagac ggcgcagcat ggtgggggag aatttgcgtt
   101701 cacccttctt gttgggcggg caaagcgcat tgaacagttt ttcatcatcc tgttcttttt
   101761 cgtgcatcac gcgtacatcg agcgcggctg ccacgaggtt gtgcgaagcg gtaatggcgt
   101821 ggatgtcacc cgtcagatgc aggttgaaat cttccatcgg gatgatctgc gaataaccgc
   101881 cgcctgccgc gccgcccttg atgccaaagg tcgggcccat cgaaggctgg cgaatggcgg
   101941 tgatcacctt cttgccgagg tgggcgccga gcgcctgtga aagaccgacc gtggttgtgg
   102001 tcttgccttc gccaagcggg gtgggagtga tggcggttac gtcaatatat ttgccgtttg
   102061 gtttattctt aaggcgttcc aaaatttcga gcttgatctt tgccttgtac ggcccaaaca
   102121 gttccaattc attcgggcgt acgccaagtt cttcggcaac ctgcaagatc ggtttcaatt
   102181 ttgcggcttg cgcgatatca atatccgatg gcacagggcg caccaatttt aatttggtgg
   102241 gagtgaatgc ggcttttgcc ttgggggcgg ctttctttac cactgccttc ttcacaatct
   102301 tccgcgcctt gggtgcggcc tttgcttttg cagacttctt ggtagccata gaatctcctt
   102361 aaattatata taatccagct tgtctattat ccaagttttt ttggaaaagc gcaagagaat
   102421 ttcatcccaa atttgtcact aaaatggatt cacttcaata acgtccccac attttcaccg
   102481 cgcaaagccc gagtgagcaa tccattctcc tcagcagaga agatctgtac agtcaaattc
   102541 ggattctttt ggatgaggtc gagcatttct tcgactttgg ccttcatccc gccggtgaca
   102601 tcggtgctga cggaaccgcc aatgccagcc cgcatctttt cataatctga caactgaatc
   102661 tgtttcacaa gcctggtccg cgctgggaaa tcttcccata cccctgcttc aattcctgca
   102721 agcagaatac gggtggggga gaattgttca acgaggaagg tgaacacatc ttcagtggag
   102781 aggatcgtgc cgccgagtgc ctcatcaaat gcaacatcac catacaccac gggcagtatg
   102841 tgaaagtcga aagatttgcg tatagtcaaa gaattatgcg ctataacttt tcggttcttg
   102901 gataccagtg atgaagatgg tgggaacgag atcgccggga cgcctgcgtt gtaaagcgct
   102961 tccatcacat agcgattcaa ctccgccgcc tgaaagcgga cttcggcgaa gccctgccat
   103021 tgttccgcgg tttggactcc gttgcgggtg ccatgtttct tcgcggcgac gtggccaaaa
   103081 gagccagatc catgccccag aatcaggagc aggttggggg ttgaatccag aaccgttttt
   103141 atttccaggg ccagttcttt tagtttatcc agtcgggggg tgtaggggat gtccttgtcg
   103201 gtgatcagtg atccgccaag tttgaggaga acgatttcgg ttttcatttc cccatttaac
   103261 cacaaagtgg tgaagacacg aaggcacaaa atgctataat ttcatttatg gatattcatg
   103321 gtggaatcat tgctgcggca atactggttg cggttgcggc cttgttcgtt gtgcgtgctt
   103381 cggtgcgcgt gatgcagaat gcgcggaaga tgtcttttta cagcctgcgg cggatgcaca
   103441 atgcaaatgc gctgcggctg tttttatttg cactggtttt gttcgggtgt tcgttttggc
   103501 ttccgttctt tggcgaaccg atcgtgtacg acttttttcc gccatcgccc acggcttcgt
   103561 tgacccccac cattacactg acgccaacca tcacgctgac gccgaccatt acaccgattc
   103621 cttcagaaac gcccacgcct gcagtttctg atacgcccac gctgaccttt accccatttt
   103681 tgcccgtggc cattgaagcc ctgttcagcg gacctgtgac tccaaaccca gatgcattat
   103741 ttacgccgct tcaattttca accgagttcg acggcttgaa ccctgtagac ccgaaaacgg
   103801 tttttgaact tccgatccaa aaaatgtatg gcggcttcga ttacaacaac accctccccg
   103861 gtgtgcagtg gaccgcgctg tggtaccgcg atgggcaatt ggtctgttat gaaaccacac
   103921 cgtggaatgg cggtacgggc ggcatcgggg gttatacaga gtgcgatgac cccatcggcg
   103981 ggtggcaggc ggggacatat gaagtgcaga ttttcatggg ctacgattgg aaagtggtgg
   104041 gtcagtttat tgtgcatagc gaactggcaa cccctactcc aaccagcacg ccgacgatga
   104101 ccccgactcc ataattaaat ccccacaaag gaatgaaggg ttacaaagga aaaccatgcg
   104161 cttctttgtg accctttgtg acccttcgtg gttaaattct ttcaaaggtg aattacatga
   104221 ccgataaagc atttcaagat tattaccccg agtattttgc ccattgttat ggctgtggca
   104281 gtgtgaatga acttggtcac cagatcaaga gtttttggga tagcgaagag tcggtttgtt
   104341 attttcagcc gaagccgtat cacacggcga ttccggggta tgtgtatggc gggctgctgg
   104401 cttcactgat cgattgtcac ggcacaggga cagcagccgc ggctggctat cgtgctgaaa
   104461 accgcgcaat ggataccgaa ccgcctttgc gatatctcac ggcttcattg cacgtggact
   104521 atctcaaacc cacaccgatg gggccagtgc tggaagtgcg cgcaaatgtg aaagaagtca
   104581 aaggccgcaa ggtggtcgtt gaagaatgga tcgtggtcaa tggcgtggtc actgtgcgtg
   104641 gggaagtggt ggctgtgcag gtaccggaga agttggtgct ggagttattg gagggaaagg
   104701 atgggggatg aaagaggaaa gaagaaagaa gtggaaagta gaaagtggtg ttttgaaata
   104761 aaagtgtctc gcgtggatgg tcatttttcc agaccaccgt ccacgcgaga cattaattac
   104821 caattaccaa ttaccaatta ccattcactt tctatccccc aaagaattcc ctcatcggct
   104881 tgaagtaaaa atcctcccag ccttgctggt acccctctgc ctgacctttg ggaatgtggg
   104941 tgtgaatcaa ggtcagtttt gtttttccgt ttgcttcttc caataaaatt tcaacgtgtg
   105001 aatcaactgc gtcttcgggg aattcagcgg tgtgccatgc ctgaacgatc tttttgtttt
   105061 cttccagttc cagaaacgtg ccccaaatat aaccgtccca ggcggtgaac tcaccgttga
   105121 ctttgccatc cactttggcg gggctgccgg tcatcagggt atgtccctgc gtggagagaa
   105181 acgcctgata gattttatcg gcgctggcat tgaatgttgc tgtgagcgta aattcagcct
   105241 tcatcatttc ctatatcgcc cattctctct tttttccgct catttctttt gcgatctctg
   105301 cggcgaggcg ggcgttgttg agcaacaggg cgagattggc tttgagcgat ttgcccttgg
   105361 tcaattcgct gatgcggccc agcaggaatg gagtgagttt ctgtccgtgg attccctgcg
   105421 cgatggcttc agcagacgcc tgagcgatca tcggttccat ttcagacttt gaaattgctt
   105481 ctgcttcggg aattggattc gtcacgagga ttccgctctt catgcccaaa ttccagtggg
   105541 ctttggcgaa gtcggcgatc tctttggggg tgtccaggcg ggcgctgact ccgagtccgc
   105601 tttcgcggga gtagaaagcg gggaattcgt cggtgcggta tccgatgaca ggcacgccca
   105661 tggtttcaag atattcgagt gtggcgggca gatcgaggat cgcttttgcg ccagcacaga
   105721 cgacgatggt gggaatctct gccagtgagc gcaggtcagt ggagatatcg aagggcgatt
   105781 ctttgtgaac gccgccgatg ccgcccgtgg caaagacttt gatgcccgcc atgtttgcca
   105841 cgaacatggt gcctgccacg gtggtgccgc cgtccgcttt tttgacgatg gcggtggcga
   105901 aatcgcggtg gcttactttg agggttgagt ctgcttcaga aagccgaacc agttccaggt
   105961 cagagagacc gatgcgaatc tctccatcca ggagggctac ggtggcggga gtcgcacctg
   106021 cctcgcggat ctgcttttcc atatcccgcg ccagttccag gttttgcggg tggggcaggc
   106081 cgtgtgtaat gacggtggat tcgagggcga cgagcggcgc gccatgattg agtgcgcgtg
   106141 agacttccag cgagagggtg aggttgttct ttttcactat gattttctta tccaatggtg
   106201 ggtgagatgt gagatatcga agagcgcctt ttcgatcttg agaaggtcgg cttgtttttc
   106261 ttcgaaaggt ctgttttctt tttcaatgag ttccttgcct gccagcagtt ctttgcggaa
   106321 ggctttgaga atttccagcg cggtggcggt ggcggtggaa tctttgggtc ggctttcccg
   106381 cggattcaac tggcctttga ggtctgggtt ggcggcgatg aaggcatccc attccttgtc
   106441 ggcttctttt tctttggagc cagagtagga ggaacggtag ctgccgctga cgggtctttc
   106501 gtagttgcgg atgaccacat tttcgatcag gctgacgatt tcagcggttt caaaacgttt
   106561 gcgcagacgc tctacactgg cggccttttt tgcgttttca cgcgcaaggt tccaggcttc
   106621 cttgtgcttg tcgagaatga gtgaggatgg gagctgccat gtgccgatgt cgatccattc
   106681 aatgtagatg ccgaccgcgg cggccttatc ttgaaatggc ttttcgaaga attgggcggt
   106741 gatgcgcgaa cgtgatttga agctgggtgc cgaggcggct tttttgctgc cgagggcggt
   106801 ttgctggcct gtcaactcca cccgcatttg ggtgatggtc tgctcattgt tggaggtgtt
   106861 gtcaatttcc ttctggccga tggtgacaag gatctcgctc aaggtgtgag aggtgatcag
   106921 gttttcaagt tcggaggtga tgagcgggat ggcagaggta tcccacggaa aactgaccgc
   106981 cgagggagtg gatggcacag gcgtgatgat ggtttggttg tagacaagcg ccttgaccgc
   107041 ctcttcatcg aagagataag agctatcggg cgcttcgggg gtttcgggtt ttggtttgcg
   107101 aaggatgctg aagatgacct tgatatcctg cgcttcgatg gggatgccgt ctttggtgcg
   107161 cgatttgacc gtaaggccgc tgatgaattg gtcacgcagg ttgatgatgg cgtattcgcg
   107221 tttgcctact tcgtcaaatt tgccgatctc gcggatgcgc tcgaacctgc cgagtttcca
   107281 cgcatctttg cgcggataga tcacttcggg ttcaccgttc actttttcga gcagggccac
   107341 actatcgagg ttcacttgga tcgcacctgg cccgccaata aggatgatgg gggaggtttc
   107401 gtctttttcg accacgcggc cttcgttgat ggtgatttta tcgaggccgt aaccgaaggt
   107461 cacttcttcg aggaattggg aggcgagttc ttcatcgtgt aattcgaaaa tatcatcaag
   107521 gtacagggat gccattttcc gggcgaggct gtacacaacc gccatgagcg gcgcatactt
   107581 caccagactc atgccaatga caactgcggt ctgccaggtg gcctgtgtga agaaaacagc
   107641 cgacagcatg gaaatgccgc taagcgtcag ccaaacgacg atctgcgcga tgctgcgcca
   107701 gcggtttcta aaatccacgc cttcatcggt aaggctgaag agcagggtgt tgagttcatc
   107761 acggcgggcc ttgttcatgg ctggcctccc tgctggtttc gtggaccatc ggggttgtta
   107821 tccaaaagcc atttccaaat gtcatcgagc ttcctcaact ctttttcaac atcggtgttg
   107881 gcactgcttt caaaaaggat gaactctttc aacggctgga tgagcagttg cagtgttttg
   107941 aatgggtttt gctgcggcat ggtgggtttg ccgctgaatt ggcgtgaggc aatgcgtgca
   108001 aaactcttgc tgccttcatc gcgggaggcg gtttcaatga gcgcctcttt ttccttgagt
   108061 tggccttcct cttttttggc gaggttcatc cactcggtgc tccattgtga gatgacctga
   108121 ttctcgatct cctgttcgaa gagtacgttg tgaatactaa cttcctgaat ttcaagtccg
   108181 cgtgattcga gttgtttgaa ttcgaggctt tcgagccatt cacctgtggg cgagcccgta
   108241 tcatccagcg ccatcacatt gctttgtttg acgcgcttgt tgatcatgtc ttcgatggtt
   108301 tgcaatccgc tgaccccgct ggaggtgaac aggtcactca gtttgaattt gcggatgtat
   108361 tcgcgccaga tgttcacaac cagatgcgca ggcagtttgt tccattccat gcgcaccttg
   108421 gtgttttcgg ttgtgccaag ctggatgacc tcacgggtga tggcattgcg cactgcttcg
   108481 gcatcgaacc catattgtga ggtgactccg ctttcggtcg gtttcttttc aacgggacgc
   108541 ttgatactga acttgatgct gatcgtgggg gagacctcga acccatcgcg ggtcatgccg
   108601 ctggtttgct ggcggcgctc gttactggcc ttggatgggt tggaagcagg gtttagaaaa
   108661 ggctggtcgc tcgccagtgg accgatgaac tgccactgtg aacgcaaatc aacactgcct
   108721 gcaatgtact cgttcccact ggtgaacttg atgcccgggc caacagtatc cttgatttcg
   108781 gtatccgtcc gcaatacgag ggcgctggcg gtatcaagca cgatcacgcc tgcaccgcgc
   108841 ttgcctgctt caccttcatg caggatcacc cgtccgtttt tcacaaaaag ggcggggccg
   108901 cgcctgccag attcaaaatt gctcacacga gcggcaattt cacggcggtg ctttgggttt
   108961 tgaatgggga ggacgaactg ggaaaagaag taaatgagtt tgtcgcgcag atagaacgcg
   109021 gcgaaaatat caagcccaag gattacggcg ctggcgaagt aattaaaagg gattttcgcc
   109081 accaacacca caatatgcca gattacattt gttgtcagta ttataccgac cggggtttgc
   109141 aggaatgcgc cgattccgct gaatattttt cgcatcttgg gctgattcta aagcacaaaa
   109201 tggtaatttg gcaaacgagt tgcaggggta tgaaagggtc atccccactt gttacttgat
   109261 ttttatgtag tttgacttat actggatttg tattgtccac attacacgcg caaaagagga
   109321 agaaatggat acaaaacgcc aaaacctgaa gggcatgatc ctggtcggcg gggcggttct
   109381 tgtgattcta attgccgtct ttgtcttcag aaatttcaat acacaaggag cctctgcaaa
   109441 agcaccgatg agcgcaaccg tcaccctgag catagaccaa ggttcgtttg cagccagcga
   109501 cgatgtcctt gtgcatgtca ccatcaccaa ccccaacgac accccgatcc gtattctcaa
   109561 atggtttacc cccataaatg gactggagcg ttcattattc accatcaccc gtaacggaga
   109621 aaccgttccc tacatcggta gaatgctcaa acgtgccgag cccaccgaag ccgattacat
   109681 cacactggag gcgggcggca gcatcactgc agatgtgaac ctggcagaat attacgccct
   109741 gtctgccacc gacgattatg aggtcatgta cgatgtcaca tcgctgcaac tttacatcga
   109801 aaaagaaatc ggtgagttga ccaatggacg actgagttca actccgctta aaatgttcat
   109861 cacagggcgc gcagcacccg ctcactaatt cagttaatgc gccctgcaaa tttacccatc
   109921 ggaggaataa tgaagatagg aaatgcacgc aagttgtttt atgtggccat gattctgatg
   109981 ttggccctcg ccgctttcgg ttcatgggat gtatccgccg cccgcgccca aggctcattg
   110041 ggtggagcca ctgtcagatt aagtgtggac cgcgcttctt tcgggaccgg tgacagggtc
   110101 ctcgtcaaag tgaacatctc caacccgaca aattcccaga tcaaaatcct caaatggctt
   110161 acgcctgcca tgggagttga agcgccgatt ttctccatca cccgtgatgg aaaagctgtt
   110221 gcctaccttg gcaaacttgc caaacgcatg gctcccaccg atgccgatta cattaccctg
   110281 ccgcccgggg gaagtatttc cagcgaagta gaccttgctg aatattacga cctttctgct
   110341 tcgggcaatt attcggtgca atacaacatt gcctcggcga atttataccg ctgggaccag
   110401 tcacaaagca ccgacctgtt ggtttctgct tcagttgacc tgttcgttga aggccgcgcg
   110461 aatcgcgtca ttcagcgtgt ggatccgcaa gctgtggtgg gcacaaatac gtttaacggc
   110521 tgttcctcct cccagcagac agatctggtg aacgcccgca atgccgccag cacatatgcc
   110581 gccgatgatg tggcttattt tgcggccaac aaacagggtg agcgttatgt cacctggttt
   110641 ggcgtgcttg attccgcccg ttacaacctc gtgaaatccc atttcaccaa tgttcaaaac
   110701 gcggtggata cggccagccc catgcatttt gactgcactt gtactgatgc tggtgtgtat
   110761 gcctacgtgt acccggattc tccttatgag atctatttgt gcggagcatt ttggagcgcc
   110821 cccaccaccg gcacagattc gaaggcgggc acactcattc atgaaatcac ccacttcaca
   110881 gtagtggctg gcaccgatga ctatgcctac ggccaaactg cggccaaggc tctggcgatc
   110941 agcaatcctg cccaggcggt aatgaacgcc gatagccacg agtatttctc tgagaataat
   111001 cctccgcttg aggtttcttc aggtaccttt acggatgtcc cctccacgta ttgggcttat
   111061 aactttattg agcgtttata tagcgctggt atcaccggcg gctgtagttc ttcgccgatg
   111121 atgtactgcc ccgaatccag tgtgacccgt gcgcagatgg ctgttttcat tttgcgcggt
   111181 gaacacggca gtgcctacgt tcccccggcg gctacaggta cggttttcgg cgatgtatcc
   111241 accagtactt tcgcggcggc gtggatcgaa caatttgctt ccgaaggcat caccagcggg
   111301 tgcggcggcg gtaattactg ccccgattcc aatgtgaccc gagcccagat ggcgatcttt
   111361 ttgttgaagg gcaaatatgg ctcggcttat gtcccgccgg tggccacagg catcttctct
   111421 gatgttcctg tcggctcttt tgctgctgat tggatcgaac agctcgcagc ggaaggtatc
   111481 accagcggat gcggcggcgg caattactgc ccgagcgcaa acgtcactcg tgctgagatg
   111541 gcagtgttcc tcgtccgtgc ctttaacctg ccgtagtaaa gaatgttttc atggtgcctc
   111601 tgatttttca gaggcacctt tttgtttaat atgggtacga aaaactcgta tgaccctttt
   111661 ggggggctgg tttctcattt gaaatttttg tttttcggct tatactaaaa ttaccaacca
   111721 gaaggagatt cggactgaat tgttcaatgt tgtgggaaat agattttcat atgtggattc
   111781 aagctgcaaa gggtatcaac catgagtaaa gcgttttttc gggtaattct caaggcaaag
   111841 gggttacttg tctttttgtg cttcattttt ctgacaggat gtgctggagg aacgggtgcg
   111901 cccgccaata aaactgaatt ccagcctttg tcttcagaca gcacttcttt ttattactac
   111961 gtgagcgaaa accgagtccc tctaacctta tccacagaat ggatcgcagt gcaatttgcc
   112021 aatgaagatc cttctgcaca ggcttcagct ttgaagggtt ccatcgtaga tgcgaatgga
   112081 caggcggagc aattgacccg atatggagtg acacttctgc ccgtgcaggc gggaacaacc
   112141 tttgaaactc ttgtagcggg aatcaatgcc ttgcgttccg attcaacttt ttttcaagta
   112201 aacccggtct ttcagtcggg agatacggga atgatcgtga cagatgaatt catcgttact
   112261 tttcctgcag ggatgagcat ggatgaaatc aatgctgtta atgcttccta cggtgtagag
   112321 gtgggtgatc ccatccttgg gcaggaaaat acctttgtgc tgcgggcaac cactgcaacc
   112381 aaaatggata cactctctct tgccaatctg tatcaggaaa gcggcgtggc aatgtttgcc
   112441 gctcccaatt ttgtgcgtgt tcgatagtcc catcccttgt gaattaacca gcaagtttgg
   112501 ttaaaatttt tgttgtttta gaaaaggaga atgcaaatga agaatagcaa gacagttcgt
   112561 ttattttcaa agctgctgac agtaattgct ttggcagcca tggtattttc cacctttgtg
   112621 tcggggaacg ttagtgccgc ccaggcaaaa ggcgcacagc cttcgatttc aagccaggct
   112681 gcagatggtt ccttctatta ctatgcagat ggcaaacgca ttgaactgaa tccgtctttg
   112741 agttgggtgg ctgtacgttt tgcgaatgat gacctcggcg ctcagtctgc cgcggttgac
   112801 aactccagcg cattgctggg gtcccttgat caggctcgtc gaatcacccc ctcaagcctg
   112861 gctctgctgc ctctcaaggc aggtgtaact gcagcgacaa ttcgtgatgc agtcaaggcg
   112921 ttgcgtgcgg atacagccgg ttttgcattg gtgaacccgg tcttccagac ctccgatgct
   112981 gaaatggcca tttctgatga atttgtagtt accttccctg cagggatggg aatggatgag
   113041 atcaatgcca tcaacgccgc caacggtgtt gaattggtcg atgccatttt gggacaggat
   113101 aatacctttg tgttgcgcgt cactggcggg gccgacctgc ttgagatggc gaacaagtat
   113161 caggaaagcg gaatcgcaat tcattccgcc ccgaacttca tccgcattgt tcagaagcct
   113221 gctcccgctc cttcgagccc caagccagat ttcgctccga tggctggaac gaacgatacc
   113281 tactatccct atcagtggca cttgaacaat atccagcagt atggtgctgg aacaactctc
   113341 gatgcggata ttgacgcccc ggaagcctgg gacctcacca caggcagttc ctccatcatc
   113401 attgcagtgc tcgatgaagg cgtggacctt tcgcacgaag actacagctc caagttggtc
   113461 cctggctacg atgccaccgg cggcggcagc tccggcggac catggggtaa tgatgcccac
   113521 ggtacaaatg tggctggttt ggctgcctct ggcagtaaca acagcctggg tgtagctggt
   113581 gtatgtcagg cttgtaagat catgcctgtc cgtattgcct acagcgatgc aaatggcaat
   113641 tgggtgacca ccgatgccac gatcgcgaat gccattacct gggcttatca gaatggcgcc
   113701 gatattttga acaacagctg gggcggcggc tcggaagcga ctgtgatcaa cactgcgttc
   113761 tccaatgcca agaccctggg ccgtggtgga aaaggctctg tgatcgtgat ttctgccggt
   113821 aactccaatg atcccacagt aagttacccg gcctacctca gcactgccat tgcagttgca
   113881 gcttcaaacc tttgcgatca acgcaaggca cccatcaaca acctgtgcaa cggttatgaa
   113941 tactggtggg gcaccaacta cggcagcgca ttggatattt cttctccggg tgtttggctg
   114001 ttctcaacag atattaccgg tgcagctggt tatgcttctg gtaactactt tgaccagatg
   114061 aacggtacct ccggtgcagc tcccattgtt tccggtgtgg ctggcttggt tttgtcggtg
   114121 aatccgaatc tgacctctga tgaagtgcag tcaatcctcc agagcaccgc tgatgatgtc
   114181 aatggcggcg gctgggatgc cgagatgggc tatggccgtg tcaatgccta caatgcggtt
   114241 gtggctgctg gagctggttc caccactttc tccgatgttc ctgccaccta ctgggcttat
   114301 gatttcatcg aacgcttgta caacgctggc attaccagcg gatgcagcac ctccccgatg
   114361 atgtactgcc cgacagcttc tgtgacccgt gctcagatgg cgatcttcat cctgcgcggt
   114421 attcatggca gcgcctatgt acccccggct gctaccggta ccgtcttcag tgatgtttcc
   114481 accagcacct ttggcgccgc gtggatcgaa caattcgctg cggaaggcat cacctctggt
   114541 tgtggcggcg gcaagttctg tccggatgat gttgtaaccc gtgcccagat ggcgatcttc
   114601 ctgctcaaag gtgagcatgg atcttccttc gttccgcctg ttgcaaccgg tatcttcccg
   114661 gatgttcccg tcggttcctt cgctgcggac tggatcgaac aactctacaa tgaaggcatc
   114721 acgagcggct gcggtggtgg aaactactgc ccgaactcat tcgtgctgcg cgatcaaatg
   114781 gctgtcttcc tggtaagggc tttcaaccta ccgtaagcca gacctgtatt agcaaaaaaa
   114841 gcgtccctga attttcaggg acgctttttt gttggtggtc ttgaaaatcc ttccctttgt
   114901 tatatactgg aaattgaaat aaaaaaccca tcataggagg aataaatgct ccaaaagact
   114961 ttggttgtgt tcgcacgaaa gcccatgtat ttgttgatga tactgggtat tatcttttca
   115021 acctttggca cggctcaggc tcaggaattc aatggaagta ttgttccagc tggatcgacc
   115081 cagatacagg tggatgatga agtctttgcg agtctggggc agaagggcga ggcgcgggtc
   115141 attatcaact tgcgcgatcc cgtttcgata gccgctccct ccaatgagcg gacatctgcg
   115201 attgcccgta ttcagggaag tgtattgggc aggttgagtt ctcaagattt tcgggtgctt
   115261 cgtcaatatg tgaatgttcc agcgttggca ggtcatttga catccagcgg cctggatata
   115321 ttgatgtctg atccgaacgt gacctccatt gacatggatc agatgggtgg ggggcacctc
   115381 tctgaaagtg tgactgcgct tggcgctgac attgtccact cgaatggata tacgggcgcg
   115441 ggcataactg ttgctgtgtt ggataccggc attgataccg atcaccctga tttgagcgat
   115501 gatctcgtgg cgcagcattg ctttacggat tctcaatgca agcccgggtc cactgccgaa
   115561 ggtacaagcg cggaagatga aaatggacat ggcaccaatg tctctggcgt catttcttct
   115621 gggggtgtgg tcagttcggt tggttttgct ccagatgcca gcattgttgc agtgcgggtg
   115681 cttgatgcaa acagcagcgg ctgggtctca gactggctgg cagggttgga ttggatccgt
   115741 acaaatcaag gcacactcgg cgttgatatc atcaatatga gcctcggaac ttttgccctg
   115801 tacagcggcc attgcgatgc ggataattct tcagtggcga atgtggttgc gcaattgaat
   115861 tcactgggga tcgtcatttt cgcttccaca ggaaatcaag gttcttccac gcaaatggcc
   115921 atgcctgcct gtctctcagg agtcgttgcc gtgggtgcga ctttcgatag cgacctgggc
   115981 agggaaccag attcaggcac gtatcaagac atctttggtg gaaactggcc tgcttgtgcc
   116041 aatgacacca ccaacctgca gaccatcacc tgtttcacta acagcaattc cgtgatggat
   116101 attgttgcgc cgggcgcaag gatcactgca cctggcatgg gaggcggtac ttccacatac
   116161 tcggggacat cgcaagcttc gccgaccgcg gcgggtattg cggcgttgat gcttcaagcc
   116221 agcccgggct tatctcctgc caccattgaa gcaaccttga aaagcacggg gacttctgtc
   116281 acagatgcca aaaatggatt gagcttcccg ctcatcaatg ctttggctgc cgtcaacgcg
   116341 gtcaacacca cgtttacaga tgtgccatcc acatactggg cttatgattt cattgagcgt
   116401 ttgtatgccg caggcatcac cagcggatgc agtacctccc caatgatgta ttgcccgctg
   116461 tcaccggtga cacgtgctca aatggcgatc ttcattttgc gcggggaaca tggcagtgcc
   116521 tacactcccc cggccgccac gggcacagtc tttagcgatg tcaccaccag cactttcggc
   116581 gcggcatgga tcgaacaatt tgcatccgaa ggcatcactt caggttgtgg caatggcatg
   116641 tactgcccgg atgccaatgt gactcgtgct caaatggcga tcttcctgtt gcgcggcaaa
   116701 tacggctcgg cttacgttcc gcctgtggct acgggcgttt tcaccgatgt tcccgtcggg
   116761 tcatttgccg cagattggat cgagcagctt gcggcggaag gcatcaccag cggatgcggc
   116821 ggcggtaact actgtccgaa cgcagaggtg acacgcgacc aaatggcggt ctttttggtc
   116881 cgcactttca acctgcccta gtaaattcaa tatccaatca ttggagccgc gagaccatgg
   116941 tctcgcggct tttttcttac aaaacaaatt tgcaagcaaa agattttttt gtagggtcaa
   117001 tgggggattt gttttgagaa ggggtgtgtc ataatttttc aaaaattctg gaggtgttta
   117061 tgaatcgaaa aatgctgtgt gtgttgatcg ttctgttctc tttcggggcg gtgcatgtcg
   117121 tttccgcccg gccagctttg gcgttgattg attcgccgcc gtcgcttgga ttcgctgaaa
   117181 tcgccagtgg attgtcttcg ccagtttcca tcaccaatgc aggagattct tccggacgat
   117241 tgttcatcac ccttcaaggc gggcaggtcg tcatctatga tggctcgcaa attttgccca
   117301 caccgttttt ggatgtttcc tcgctcattc gaaatggcag tgagcagggg cttctgagcg
   117361 tggcgtttca ccccaattat gaaaacaatg gatacttcta cgtctattac acccgcttgt
   117421 cggatgcggc gttggtgatc gcgcgttacg aagtttcagc ggatccaaac atagccgacg
   117481 cagggtccgc ttcgatcttg ttgaccatcc ctcatgcaga agcctcgaac cacaacggcg
   117541 gacagttgca gtttggtccc gatgggtatt tatacattgg cactggtgat ggcggcacag
   117601 gcggcgcata ttcacaggat ggtttgtctt tgctcggcaa gatgctgcgc attaatgtca
   117661 atggcgcggc gccttactca attcctgccg acaatccctt tgtgtctgac ccgtcagttg
   117721 ccgatgagat ttgggcattg ggtttgcgca acccctggcg cttctcgttt gattctttaa
   117781 caggtgattt gatcatcgcg gatgtgggtc agaatgcctg ggaggaagtg aatgtgcagc
   117841 ctgcatccag caacggtgga caaaattatg gctggccctg ttatgaaggg atgcatgata
   117901 atggcgatac ttccacctgt accatcggaa cgatcaccag ccctgtggtg gaatacgagc
   117961 atggtgcatc agataccaac ggctgttcca tcagtggtgg ctatcgttat cgtggaagtg
   118021 aattccaaag cctgaaaggc gtttacctgt atggcgattt ttgtacaggc cgtatttggg
   118081 gcgcggagcc aagcaacggt tcatgggttg ccgtggaatt ggctgatacc gattatggtg
   118141 tcaccacctt tggtgaagac gaaaatgggg aactgtatgt tgccgatttc tttggcggcg
   118201 taatctataa aattcaagcc accaccttca ccgatgtgcc gcttgatcac ccccagtatg
   118261 cctatattga ggctttgtac gatgcagggt ataccgcagg atgcagttcc agtccgctga
   118321 tgtactgccc tgatacgatc cttgaccgtg ctcaatcggc tgtgttcatg ctgcgcggac
   118381 aaatgggcac cggctacact ccacccccag caccctggga taccttcacc gatgattgga
   118441 ctggttttga atgggcacaa ccgtgggctg aaggcatgta tcaggaaggt ttaacaggcg
   118501 ggtgccagac cagcccattg atgtattgtc ctgcaaccca gcttccacgt gtggaggcaa
   118561 gtgttttcgg tttgcgaatg aagtatggcg tcaattacat tccgcccgcc gcaacaggta
   118621 ctttatttgc cgatttcccc gccacagacc caagttactg ggcaattgat tgggccgagc
   118681 aggcttattt ggatgggttg ctccctgcct gtggaaccga tacggtcagc ggcaaaccaa
   118741 tgttctgccc gaacgaattg gtggaccgcg gctggggtgc attcctgatc gtcaacgcta
   118801 aaaatcttcc gttgccataa gccaataaaa aaacaaataa caggcgttca ttctcttctg
   118861 aatgaacgcc tgttgtggaa ttggccagag tcatttcaaa aaagacaggt gtgtcgttac
   118921 ggcaggttgt tttgtaagct cacagaaagg ttttatctca attcacagtt gattatcaca
   118981 ttgccaaccg acatttcatt tttgttttta caaaaaaaat gtccctgaca atattgtggg
   119041 tgattctaac aattatgccc gatatacatt tcaaacgaac atgccgataa ttatattaat
   119101 gaaaaagagg atggtatgaa aaacagttcg cattttttcc gttttgcttt ggtggtgatc
   119161 atgttggttg ccatttttgc aaatacgcct gttgcttccg cttctaattc agcacaaaca
   119221 caaaaggcgg gggcacagac ggggtttgta tctgatcttt acaataccgg cgatcctgta
   119281 ttgaccgata tttgggtgga tgcggtcaat ggcaatgatg ccaatgacgg ttcctcccct
   119341 gcatccgctt ttcgaacgct caccgctgca tggaacagca ttcccacaac caatccgctt
   119401 ccacaggggc ggagaattaa cctgcaaccg ggcacctaca ccgaaagcat gatccccaat
   119461 tattgggaaa accgctatgg aaccttcacc gctccgatct tcattcgcgg caatggctct
   119521 tcacggggtg aggttgttct gcagggcacg atcagtatgt ataacgtgaa ttacatctac
   119581 tttgaaaatc tgagcatgat ttttaatggc gatgtatttc attgtgagtt gtgcaatcac
   119641 attctgttgc ggaatacggt tttgaatggc ggtatgttcg cacacgagac catcaaggcc
   119701 aaccaatcgc agcatcttta tgttgagaac agcgatattt cgggctccta tgagaatgcc
   119761 attgactatg tcgctgtgca gtatgggcat gtggttaaca atcagatcca cgatgcggat
   119821 gattggtgcg catatttcaa aggcggttcc gcttacctgc gtttcgaggc aaatgttgta
   119881 tataactgcg gcacaggcgg ttttacagca gggcagggga cgggattcca atacatggtt
   119941 tctccctggc ttcaatatga aacctacgat atcaaggtcg tgaataatgt cattcacgat
   120001 accgatggcg cgggtttggg tgtgaatggc ggctataaca ttttgatggc ctacaacacc
   120061 ctgtatcggg tcggcagcgt gtctcatgtg atcgaggtgg tttttggagg ccgtacctgt
   120121 gatggtcagg cgggtgaccc tggtcgggaa atgtgtcagc aaaacctaaa ccttggcggc
   120181 tggggcacca ctgcagtgga taacggctcc aattcggtca acattcccga tagaaatgta
   120241 ttcatttata acaacattgt ctacaacccg gcgggttttc aaagccagtg gcagcatttc
   120301 gccatctacg attcacgcgc aaacccggct gggacgaacg ctccaaaccc cgcccacacc
   120361 gatacaaatt tgaatattcg cggcaacgtg atctggaacg gtgattcggg catggtactg
   120421 ggaatcgaag gcaattccga tgcctgcatc gccagcaacc cgacttgtaa cgaaacccaa
   120481 ctgcgtgccg ataatgccat caatacggtt gaacccttgc tggtgaatcc atccgcaggt
   120541 gattttcaca tcaatggcac ctgggcggcg ggtgtcacca cctatgatat tcccgatttc
   120601 gtttgggata tcggcggcgt gccaggcggt gacaacagca atgcagttcc tgttgatttt
   120661 gaaggcgtaa cccgcactgg cagcaatccc ccgggggcat ttttcggcgg cggctcagac
   120721 accatctttg cagatgtgac ctcctcttat tgggcctggc ctttcattga acgtttgtat
   120781 aacgctggca tcacaggcgg ttgcaacact tcgcctttga tgtactgccc tgaatcatcc
   120841 gtcactcgcg cacagatggc cattttcatt ttgcgcggta tgcatggcag tgcctatgtc
   120901 ccacccgctg cttctgggac ggtttttgca gatgtccctc tcggcgcatt tgctgttgat
   120961 tggatcgagc agcttgcagc ggagggggtg accgcaggtt gcggtggcgg caactattgt
   121021 cccgatgcat ccatcacacg tgcgcagatg gctatcttcc tgctgcgcgg cgaacatggc
   121081 agcgcctaca gtcctcctgc tggtacgggc acggttttca gcgatgtccc cgctggtgcc
   121141 ttcgccgtgg attggatcga gcaattggca gccgaaggca tcaccagcgg ttgcggcggc
   121201 ggtaactact gtcctgatgc aaacgtgacg cgcgctgaaa tggcagtctt tttggtgagg
   121261 gctttcagcc tgccatagga gaaattctga gatgaaaaca cggcttcttc aatccagttc
   121321 gaaaatggga ttgaagaagc cgtttatttt ttcagtaggt gaacctaaaa aaattgtaag
   121381 aagaaaaaat ttcagtgaat tcaccttgct gcaaacaaac aaaatcaaaa tcagtttgca
   121441 aacaagggtt gtgatcgaaa cccgattcac aacacaagga ggttgatatg tataaaacca
   121501 ggtcaatgca tttgtttcca aaagttctgc cctcgatcgc cctgacggtt ctattgttca
   121561 ttcccttttt gtcagggagc gttgaggcgg ttcgcgcatt gggcgggggc gcggaaccga
   121621 atcaaagtga tggcgatact ctgtattatt ttgcagaagg aaaccgcatt ccattgactc
   121681 catccctccg ttggatttct gtgaagtttt ctgaatccct gtcggatcaa actgcggcac
   121741 tcgaaaattt cgctccgatg gtcgggtcac tggatcaggc ccgccccatt ctttttggcg
   121801 atatcaccct gttgcccttg aacgcaaata tgaatgtgca gtcggtgctg gcgggtgtca
   121861 atgccatgcg tgcagatacg gcaaaatttt ctcaagtcta tcccgtcttt caaactgcag
   121921 atgctgaaat ggctctgacc gatgaattta tcgtcacatt tccatctggc tttcgcatgg
   121981 atgatattca taatgtcaat gcttttcatg gcgttgaatt ggtggagcct gttttgggcc
   122041 aggaaaatac ttttgtgttg cgggttgcca gtggtgtgga tgtgcttgcg atggcgaacc
   122101 tgtatcagga aagtggagtc gcgcttcatt ccgcgccgaa ttttgtccgc atcttaacaa
   122161 gcaaaccagc cgttgggcag attcctccct ccattgcacc gatgacagat gtgaacgacc
   122221 ctttatacgc aaatcaatgg tatttgaaca acacccagca atttggacca gagacaaaaa
   122281 acgatgccga tattgatgct ccagaagcat gggcactgac cactggaagt tcatccatca
   122341 tcatcgccgt gatcgatgaa ggcgtggatt taatgcatga agattacaaa tccaatcttg
   122401 ttgttgggta tgatgccacc ggccttggca gcaagggtgc gcctgttgcc gatgacgccc
   122461 atggaaccaa tgtcagcggt ttaatagctg ccagcgcaaa taatggactt ggtgtggctg
   122521 gggtatgtcc acaatgccgc atcatgcccg tccgcgtggc ctatggtgat aaatacggat
   122581 attgggtcac tgatgatgcc accattgcaa acggtattgc atgggcttat caaaacggcg
   122641 cttccatttt gaataatagc tggggcggcg ggttggaatc tacagcgatc aataccgcaa
   122701 ttgcgaatgc aaggtccttt gggcgtggcg gcaaaggttc tgtagtagtc ttcgccgctg
   122761 gaaacaataa tatgaccgtt gtgtatccag ccaatcttga aaacgtaatt acggtatcgg
   122821 cctcgaattt atgcgaccag cgtaaacagc cgatggatga tgcctgcaat ggaaatgaat
   122881 attggtgggg gagcagctat gggaattcag tggatgtttc ggcaccgggc gtcttgctgt
   122941 actcaacaga tatcactggt gcagctggat acgatacagg gaactattac gctgaaatga
   123001 atggaacgtc aggggcggct cccatcgttt ccggtgtagc ggggcttatc ctttcgctta
   123061 atcccaacct gacggcagat gaagttcaga atattctcca aagcactgca gacgatgtca
   123121 atggcgatgg ctacgatgtg gatatgggtt acgggcgcgt gaatgcgtac aacgccgccc
   123181 tggctgccaa cgccagctcc attacattta ccgatgttcc gaacacctac tgggcctatg
   123241 attttatcga gcgtttgtac aactcaggcg tcacacgtgg atgcagttct gacccgttgt
   123301 catactgtcc gctggcggca gtgactcgtg cgcagatggc cattttcatt ttgcgcggta
   123361 tgcatggcga cacgtatgtt ccgcctaccg ccagcggtac ggtctttacc gatgtcacag
   123421 ccgaaacctt tggcgccgcc tggatcgaac aatttgcatt ggaaggcatc acttccggat
   123481 gtggttccgg ggtattttgt ccgaatgata atgtgactcg tgctcaaatg gccattttcc
   123541 tgctcaaagg caaatatggc gcgtcctatg tgccgcctgc tgccacgggc atatttgcag
   123601 atgtccccgt tggttccttc gcagccgatt ggattgaaca gcttgccgct gaaggcatca
   123661 ccagtggatg tggcggcggg aatttctgcc cagaccaaaa tgtcacccgc gaccagatgg
   123721 ccgttttcct tgtgaaggcc ttcaatttgc cgtaggtaaa accagtttca aggattcacg
   123781 actccgtcag tctaattcga ttcaaggatt gatggagtcg ttgacttata taggggaaaa
   123841 gaataatatt gattcacttt aaaaaacaaa agttgtaatg taaaaatcat tggatagcta
   123901 ttcggctggt tttatcacga aatgcgacca tttgaaatga tttgttaatt gtagcaatca
   123961 tggtatgctt tacttaaacg attttttcaa aaatggttgc cacttaagga gaacagttat
   124021 gcgtaacgtt caattgaaga aaatgttttc aaagctgctt tctacaatta ttttagccac
   124081 catgatctgt tccacatttc tgtcggggaa tattgaattg gtgcgggctt tgggtgggag
   124141 ggatgcgtca tcagcccagg aggatggaaa ctctctatat tattttgcca acggaaatcg
   124201 cattcaatta actccatcct tgaactggat atcggtgaaa ttcacaagtg acgatccggt
   124261 cgctcaatct gcggcagtcg gaggttttgg tgcaatgttt ggtccgttgg atcaagcgcg
   124321 tccaatttta ttcggcaata tcacccttct gcctttgaaa gcaaatgtaa cggaacaagc
   124381 gctcatggaa ggaatccaat ccttgcgcgc tgataaagag aacttctcac aagtgaatcc
   124441 tgtcttccaa accccggatg ccgaaatggc gcttacggat gaatttatcg ccaccttccc
   124501 atcggatttc agcatggaag atattgatgc tgtcaatgca tttcacaatg ttgaactgat
   124561 tgacgccatt cttggacagg ataatacctt tttgctgcgt gttgccgctg ggacggatgt
   124621 attggcaatg gcgaatttat atcagcagag cggtgttgcg atccattccg caccgaattt
   124681 tgtccgcatc gtgaagatca acccgacgga agggcagtca ggacagcatg ttggaccaat
   124741 ggctggaacg aatgatactt attatccgag ccaatggcat ttgaataaca atcagcaata
   124801 tggttcaggg acgacgaacg atgctgatat tgatgctcca gaagcatggg atcgcaccac
   124861 cggcaattcg tctgtagtca ttgctgtgat tgatgaaggt gtcgatctga tccatgaaga
   124921 tattagtccc aaactggttg ccggttatga tgcaaccggg cttggcagtg ggggtgggcc
   124981 ttctggcgac gatgctcacg gtacaaatgt gggcggcctc gcagccgcaa gtagcaacaa
   125041 cggttttggt gtagctggtg tatgtcaaaa gtgcagtatt atgcctgtcc gtattgctta
   125101 tagtgatgcc tttggattct gggtcacaaa cgatgccact attgccaatg gaattacctg
   125161 ggcatatcaa aatggcgctt caattttgaa taatagttgg ggcggcggac ttgaagccac
   125221 ggtcatcaat acggcaattg ccaatgcgaa gacgttgggt cgtggaggaa aaggttctgt
   125281 agtcatattt tcagcgggaa acagtaattc atcagtttcc taccctgctt atctggatag
   125341 tgtgattgcg gtggctgcat cgaatttgtg cgaccagcgc aaaaaaccaa ttgctgatgt
   125401 ctgcaatgga tatgaatact ggtgggggag caattacggc agcccggtgg atatttctgc
   125461 gccaggcgtc tggttatact caaccgatat cacaggcgcg gctggttatg catctggaaa
   125521 ttatttcgct gatatgaacg gaacatctgg tgccgctccc attgtggctg gtgtggctgg
   125581 gttgattctt tcgctaaatt caaatttgac agccatcgaa gttcagacaa ttctccaaaa
   125641 tacagcagac gatgtgaatg gtggcggata tgatgttgaa atgggatatg ggcgtgtcaa
   125701 tgcatatcgt gctgttcagg cggtgaccac gtcagggtac accatttcag gcgatgcagg
   125761 catcggtggc gctaccctca cctatac
//
LOCUS       DAOQLC010000002        99676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_23_length_99676_cov_20.664398, whole genome shotgun sequence.
ACCESSION   DAOQLC010000002 DAOQLC010000000
VERSION     DAOQLC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 99676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 99676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..99676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23_length_99676_cov_20.664398"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PL000_00620"
     CDS             complement(<1..180)
                     /locus_tag="PL000_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87424.1"
                     /translation="MKKLVNKILVLFVFASLVLSACGGTAATEAPAVEATEAPAEATE
                     APAATEAPAATEAPAA"
     gene            410..1579
                     /locus_tag="PL000_00625"
     CDS             410..1579
                     /locus_tag="PL000_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC87425.1"
                     /translation="MTELPIHPLSDEHKMLRDAARDFAQKEIAPIAAEFDESGEFPYA
                     TIRKMGELGFMGIEIPEEYGGAGMDALAYVLALEEICKVDASHGVIMSVNNSLYCHGI
                     LKFGTEEQKKNFITPIATGKAIGAYSLTEPQSGSDAGAMKSRATRDGDFYILNGRKSW
                     VTSGPVANYFVVFMMTDPDKNQKGVTAFLVDGNTPGLTRGKKEPKLGIRASATSELIF
                     ENCRIPAENRLGEEGEGFKIAMTVLDAGRIGIATQALGIAESAYEAARQYSGQREAFG
                     QPIAAFQGTGFKIADMKTRIEASRLLIYNAAIAKENSKTNHGRYSLEASMAKLFASET
                     AMFVTHQAIQIHGGMGYSKELPVERYFRDAKITEIYEGTSEIQRLVISRMETGLR"
     gene            1591..3648
                     /locus_tag="PL000_00630"
     CDS             1591..3648
                     /locus_tag="PL000_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87426.1"
                     /translation="MSSGQISLIAVIIITIYLDWVLMKWLKKRKKENVEKKPVQKWDI
                     QLSSFYKPILQWISKFNFELPLIALWALWVGRVYLNFDRNMVPGYGNDDFLLSIYPYF
                     PFIRVLQCGDCFFWNGLLNGGSPTFGDSIGALLHPVMAGIMLVFGVTNGIKVAMLASL
                     IMGGWSLWWLAKVLDLKMVTRLWVSALAVVGGHLAGPLFSGDVGIIFSTAAASLTVAP
                     AVALGLHGRRRDAILFGLMVAQAILSGQGYLQLALFAGVVPALLIFGINRYLQIGKIW
                     KELALALVIAILVSSVVLLPWFTFGSQISRPTEPRSDQPLEYMPMNLVVRDMNFFMTE
                     TLGKIAAPNLYVMYIGWIPILLAIAAWRFTPRENFRLYLFFAIAIALIFFLGSGAPLR
                     WAKDWLPGYLRDIRNPNFGVGLAVTLIFGLAAIGLDQILNLKWPVVSVYNNVTKKSHP
                     IDFRWLLAPILLWSITSVYEFNSQFRVLHEWPKELVRAAESIPTQPAQWVRPVYGNWT
                     YVAAAVRHNVKITETFRIWEMIGRPNPPMSLDSGNDPAMVNDPNYLKTVEGISFVLYP
                     ENFYAFVETDTGRIPCEAIADGGHIQVKCNNAETGDLVVYENALQGWRVWRDGKSTPL
                     EEGQWLKVSAPAGNHTYKFRYWPTDVLGGFVLTLIGLGLSYWFWQKEQPSPVTTSPER
                     ESF"
     gene            3648..5705
                     /locus_tag="PL000_00635"
     CDS             3648..5705
                     /locus_tag="PL000_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87427.1"
                     /translation="MNTLSNLTKSITRAWGRLLSSPTTWYTVLIEWSIIVCAAYFFTA
                     PVLLDFDPNQLQQTGEHNESSTRPLLAEIGLLQHGEIPLWNHYMQTGFPHAGDLLGHF
                     WSPIATIPVILWGGINGMKVSIFISFVLAGLGQWYLAQVSGLKGIFRLWSSLTFMFSG
                     GLAFLWRLGWYELLVGMVWFPWTFAAFWQALKKRDITSMAWTAFCVAMILFTGGGYYP
                     FYLIASAGTMLIVALIAAQPEERFIQIKRALSIAVFSAGLIAVMALPIYDGYRLIVRE
                     SGDDLVQQGSQPVSYAVMNYVISQGEWFNTEILGTAGGWNWFYTGPLMLGALLFLPLG
                     FQHSRKRSMLAAMLAISIVLLLWVANRYPPVKYLYDYVPFLYRLRFPNRLLILAASPL
                     LILSAASLQGLMIVLRKQFKNYRISFSYQNDGLEKTTRLRGLTIIIFIVILFFSLKDV
                     YLTNHSFGFVSFPIEPKSLQALRYLKNKDEGMYYINLGGGALYWSWTPAAYIVQLPVI
                     NFNYNQHLLNRDSQNLPEAPFVATPKYQFSPTLENPPGATLLKDFDGVGLWYFADALP
                     FAFTAPGNELVNGTKLDPTHITEASAHYDGPNRVVVTTAEDHTGDVVVALVNNYPGWK
                     VLRDGEPVPLTPANEYLAAPALPGKHTYTFIFDPPLHKIGLTITLLTIFALLFFVHRE
                     QLD"
     gene            5727..7943
                     /locus_tag="PL000_00640"
     CDS             5727..7943
                     /locus_tag="PL000_00640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC87428.1"
                     /translation="MKFTSTTMDIHSHQPIPRKYWLVFALILVVLGQALMREPQPFPY
                     FDTLADMLKNWNDDAYLALSNPFNTLIGLALILLGTIVSALSTRTAPAEDYPIMGTMH
                     WEKWKRYKHWVILNLIVYGFIMVQLALHKDSVTFFWGWLAFLLVFTILFWENEHKPME
                     WITLTDAAWILGLFTLATAMGAYLLNDLPAGWIADEGPFWVAARNIAIGKTDPSFFDT
                     GVFTFPIASSLLQGWIMRWAGINMWGWRFASVLPAAGSVIPLYLLTRELFDRRTAIAA
                     SFMMIANPYFLAFARLGYNNIQALIPVTLCLYFLTLGLKKNSLYYFWLAGLAAGVGFY
                     TYFAAWLGLVAAILIILAFTLISRQSFRSGITSLLVVSTAACLVILPRVLYGASGENE
                     KSLTYKIWETGPINLFYGKLVFGDERMAEADIFNLEGVDLFYDASLYKLVFTRGFIRT
                     TAVLFDPIGYNDHPIVFGLAGPGSSIFFALGFGMALVDLKKLRHLIMLIFFLAGFFFL
                     GVLASIPPRPTHMVAILPAIAMISASGLTNFTTTVLNTIKERRIMLTTAGILSIIVLM
                     GCVQYFFLLPFAYAPPNQNDYIAWLTRQIQEPIHLYIIDNQVEPVQDSLSPHTTTMLS
                     SAEISVNPEQVSTWKNFVAFISPQSGADYAEELAAQIPGADVQAAYAPGQRLRGYVIS
                     DLPLKTSMDISLQHGITDLWNSPARSILLICGVGSIFLFAMQWLTGKTSNKMSETL"
     gene            7940..8971
                     /locus_tag="PL000_00645"
     CDS             7940..8971
                     /locus_tag="PL000_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC87429.1"
                     /translation="MKTILFHKHGGPEVLEYTDFPMPEPKNGEVLVRLKAAALNRMDV
                     MVRNGWPGLKLELPHINGADGAGEVAGLGENTKGFKIGERVVVNANLGCGDCEFCLKG
                     QDNMCLNWHLLGESVRGTYTEYICLPARQLYKLPEDFNLHQAAAAALVYQTAWHSMVK
                     RGGVKVGETVAIVGAGGGVNSASIQVAKYLGARVIVIGSNSKKLKEAEALGADILIDR
                     SKEEDWSKAVFIATQRRGVDAVVDNVGTTFPLSLRILRKGGRLLTVGNSGAPRFEIDN
                     RYIFAKHLSIIGSTMSTLADFAEVMDLVVDGRLQPIIDRTFPLKEAAAAQERLWNGEN
                     FGKITLDIP"
     gene            8972..11077
                     /locus_tag="PL000_00650"
     CDS             8972..11077
                     /locus_tag="PL000_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87430.1"
                     /translation="MNFLKRLPSFEAVLIAVILGIHIYAAFSDAYNLPNSWFTRDDAY
                     YYFKVAQNITEGYGITFDGINLTNGYHPLWMLICIPIFSLARFDLILPLRVMLLVMAG
                     FNAATAILIYRLVKSNLSNAVAMLAASFWAFNFYIHNNVYEFGLETPLAAFAVVLFIY
                     KLSQLEKDWRTQAVTKRQWLNLAGVATLVMLSRLDLVFLAILGGFWIILRGKPMRFLL
                     PLDVVIIFLSMTTSVALRTGIALYNSMFAASAVQAVVLALAVKIIALYFFGAYQHPRE
                     NSVVKTLLQAGLALTFSTMTLTGLYLLLAQLGIGRDFPRSAFLIDLGLSAFLILALRL
                     AGYWFGNRAINPQNYTDKKEKSIFNNAPLKEFQSNWKTWFTEAIPYYGIVGGALLLYM
                     LFNRLVFGTSSPVSGQVKRWWGTLGETVYDSPAPNWTAFFGLSFQGAYDAWQPASGLF
                     LWLVKVNFPKFADIPIVSDLSYTFLALLVAVAVITLFVNANRTRRAFSNMALLPLMAG
                     CGIQILAYTTTAYGGAKEWYWVSQMVFVTLLGSAVLDFLIRPLLRTKAQLAIQYVSAA
                     ACLLLAYRFGAQVVKIMPYNFFPPERPYMDVLPYLEENTPPGSIIGMTGGGNVGYFIH
                     DRTIVNMDGLINSYEYFHALQNGEAPAYLREHGMTIVFASPRLLELPPYFGQFNPYLE
                     SYSEYGGKGLLYLLDKPKY"
     gene            11173..11589
                     /locus_tag="PL000_00655"
     CDS             11173..11589
                     /locus_tag="PL000_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87431.1"
                     /translation="MKKFSFRATLLQMLVLTLTAFNLLRMWTALAWQDILTEFSTQPS
                     PIVLAVSGAVWALCGIVLLWGIWQKTAWAEKLLLGASAGYSLWYWTERLAWQNPHPNW
                     LFAVIVNLAIIVFILFSKKSLTREAYERNIENPKID"
     gene            11555..13102
                     /locus_tag="PL000_00660"
     CDS             11555..13102
                     /locus_tag="PL000_00660"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC87432.1"
                     /translation="MSETSKIQKLTELKAQSRLGGGQARIDAQHKKGRLTARERLDLL
                     LDKGSFREVDPFVVHRTHDFGLDEQKFMSDSVVTGWGTIEGRLVYVYSQDFTVFGGSL
                     GEVHAEKICKIMDMAMKNGAPMIGLNDSGGARIQEGVVALGGYADIFLRNTMASGVIP
                     QISAIMGPCAGGAVYSPALTDFIFMSRNTSYMFVTGPDVVKAVTHEEVTQEELGGAGV
                     HSEKSGVCHVAADSEADTLYLIRKLLGYLPQNNMEDAPFVQSDDPLRMDEALNSIIPD
                     DANKPYDIKEVIRMIMDSGQFYEIHENYAQNVVVGFARLGGHSVGIIANQPAVLAGVL
                     DIDASEKAARFVRFCDAFNIPIITFEDVPGFLPGTFQEHHGIIRSGAKLLYAYCEATV
                     PKLTVITRKAYGGAYCVMSSKHIRSDVNLAWPTAEIAVMGPDGAVNIIFRKELDAAAD
                     PVQKKAELVAEYKEKFANPYVAAQRGYIDDVIEPKETRPRLINALEMLSNKRDANPAK
                     KHGNIPL"
     gene            13120..14628
                     /gene="accC"
                     /locus_tag="PL000_00665"
     CDS             13120..14628
                     /gene="accC"
                     /locus_tag="PL000_00665"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC87433.1"
                     /translation="MFKKVLVANRGEIAVRIIRACRELGLETVAVFSEADRNALHVRY
                     ADEAYLIGPAPSRESYLRADKIFDVAKKSGADAIHPGYGFLAEREDFAEKCAELGIKF
                     IGPKPSSIAAMGDKGQARATVIKAGVPVVPGTEDVGNMTDDDLLRIAPTIGFPLLIKA
                     TAGGGGKGMREVRSIEEMPTLLQSARREAESAFGDGNVYLEKLIEGARHIEFQIIADS
                     FGNVIHLGERECSIQRRHQKLLEEAPSSYLDDDLRTKMGNIAVKSAQAVDYINAGTIE
                     FLVDKDRNFYFLEMNTRLQVEHPITEMVTGVDIVAEQIRIARGRQLSYTQDQIKFNGH
                     AIECRINAEDPFNGFLPSTGRITHSLIPTGPGVRVDTGVYPGFEITPFYDPMIAKLIV
                     WGETRAQAILRMRRALEEYRIVGVRTNIPFHQTLMDSHRFMAGQFDTRFVEERFSMDD
                     AVEARASSAEIAAILATLVEHQQSERSAQVVRRNERDTSNWKWVGRWERMHR"
     gene            14642..15139
                     /locus_tag="PL000_00670"
     CDS             14642..15139
                     /locus_tag="PL000_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNC87434.1"
                     /translation="MKYITTVDDQQFEIEVVDERHIRIGGRLLQVDFESVSGQPVFSL
                     LLDGHSYESFVYQGEEDWDVLLRGRQYQVKVEDEREKRLRAAAGGGIAEGGEFHLKAP
                     MPGLVVAILVEEGQDVKKGQVLLILESMKMQNELKAPRDGTIGRIRVKAGESVEQKQA
                     MLSVH"
     gene            15140..15745
                     /gene="cyaB"
                     /locus_tag="PL000_00675"
     CDS             15140..15745
                     /gene="cyaB"
                     /locus_tag="PL000_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNC87435.1"
                     /translation="MGEQETEVKFFVRDLKKVELRLLELKAQLIQPRVHEINFRYDLP
                     DGSMRTNGRVLRIRQDAVATITYKGPSDLVDGVFSRQELETQIGDFETAHKILIALGY
                     VKILTYEKFRAVYEIDGYHIMLDELPYGDFVEVEGANGAGIRAMSKRLELNIEAAVGA
                     GYARIFEDYNTRQGRASSDLTFDALRGKIPSPEELRIRAAD"
     gene            15889..16656
                     /locus_tag="PL000_00680"
     CDS             15889..16656
                     /locus_tag="PL000_00680"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNC87436.1"
                     /translation="MKLKGLDSLSKHVPELNSTSGRTRIVLYVVSLLALVTAYFIVTD
                     NIPTWSIDSEIIIASLGFLILSLFFSRKPVYQEKYKDLAYRNAFAHYALPGLCLIFGA
                     VAHAGYMNGPVIPTGWWTTVFTVLGWYWVLVGAILWIRSIATFGVDNLNLLYVYHPEE
                     SRIVDSSIYAILRHPIYASAIRVWVGLALLNGNGNSVIFSVFVPLGLTGWIRLVEEKE
                     LLERFGKPYLDYRKHVPAFWAKPRDIGKFFKFLITGN"
     gene            16660..17142
                     /locus_tag="PL000_00685"
     CDS             16660..17142
                     /locus_tag="PL000_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC87437.1"
                     /translation="MGMLKNKPDQLQKFLFKVPVWLHKMGLGGWERLIGAQWMLITTI
                     GRKTGKRRDVMVDVMDYNRNTDTYYIEAAYGAHADWYKNIQSNPIFEARVGRRKFKAQ
                     AGALSTEGAGEMLVQFYRAKPAYTRSVMAMAGMKFKDEEELRLLGKNLTLLAVKPEKE
                     "
     gene            17148..18242
                     /locus_tag="PL000_00690"
     CDS             17148..18242
                     /locus_tag="PL000_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC87438.1"
                     /translation="MEKILILGGYGYTGKLLAKYLLAQTNVQIMIAGRSLEKAKHFTS
                     ELNNPRATAIQVDAASFDSLLQALQGITLCLVAAPTTHHAEKVVRACIQARVDYLDVQ
                     YSSRKLEVLFAAKKEIEQAGLCFVTEAGFHPGLPSALIRYASKKLDVLESALIGGYLN
                     MKELPYTEAVDELMEGFINYQAQVYKNGSWTKPAAWDSRPFHFGDDIGKRTAYSMFFE
                     ELRGIPKLYPSLKETGFYIAGSNWLSDLVITPIIFVGLKLAPKRGLHPLGKLMWWSMG
                     KSKPPYVVALKAEAKGLLNGRQVEVHVRIAHPDGYEFTAIPVVAFLKQYLDGSARRDG
                     VHMMGHIAEPLRLFKDMQEMGVELNENMTD"
     gene            complement(18271..18600)
                     /locus_tag="PL000_00695"
     CDS             complement(18271..18600)
                     /locus_tag="PL000_00695"
                     /inference="COORDINATES: protein motif:HMM:NF038084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HNC87439.1"
                     /translation="MNMQDIPFGTTNWAEIAPTEHKGITGMALWRTQQFGNIRVRMVE
                     YSAGYHADHWCEKGHILLCLEGELHTELADGRKFTLTPGTSYQVSDGTDPHRSFTETG
                     ARLFIVD"
     gene            complement(18615..19403)
                     /locus_tag="PL000_00700"
     CDS             complement(18615..19403)
                     /locus_tag="PL000_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87440.1"
                     /translation="MRIYKYWAVERQTFEVQGEQKTGTFYGGSNESVEDARAKVQAKL
                     DRVKRKIAGDPRAFNGYEVDIREQVLEEINPQAVITRNRYGAKVLNVASMMILDIDKP
                     KLSLSGLFSKKDDKSKMYEMVEKLASSKYSQFGFRIYETFQGVRVIVLGRDFDPRNAV
                     TTAMMAEFNCDPLYTILCSKQECFRARLTPKPRRMGMKSMTIRFPREDTDLQTENWLS
                     EYDRRSGDFSVCRFIGQVGAALPTNDLVRLHDELTGASIGRQLA"
     gene            complement(19529..20590)
                     /locus_tag="PL000_00705"
     CDS             complement(19529..20590)
                     /locus_tag="PL000_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC87441.1"
                     /translation="MTLPPEEVILKGAAAWNKWQEENKFPPHFARPSWYECRDERGMQ
                     IKGRNELDFSGIHLTNASVHLAFAEGLNMRNAEVVGCHFEEGDFSRADFSNTTFVNTK
                     FNKTILTDAVFEGATFINCNLNRVNLVNADFRLKEIRETVVYGISAWDLKTDPAMKQS
                     RLVIEKSYELYSDLIASGKIPMMVDDIEMAQFVYYLSSHKNMRNMINILNSKGVLLLG
                     KFKDGGLERLYSLMDWFKTRNYTPMLFDFERPNSLDYTETVITMAGLAKFIVADLSGG
                     SVPQELYATLSSFQKPVIVYSDQPAYSMFKDIKRKNRFSFEIVFSDEKDLYAKMEHSV
                     KEAELGHAQLAHDLIDSHG"
     gene            complement(20706..21362)
                     /locus_tag="PL000_00710"
     CDS             complement(20706..21362)
                     /locus_tag="PL000_00710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC87442.1"
                     /translation="MNTTSFDLVIFDCDGVLVDSEPLANKVYVQMLAELGHHLDYDAT
                     LREFSGAAVFKRIEATSKKLNWTPPVNFLPEFFSRLSELSLRELKPVDGIRQLVESLT
                     VPVCVASNGARDEIVLRLKLANLTEFFGNAIFSGLEVPHPKPAPDVFLAAAQAFHVPP
                     SRCIVVEDSVPGVTAAVRAGMRVYGHAAITPVEVLREAGAMPFRDMRELKRILSAQAV
                     "
     gene            complement(21373..22005)
                     /locus_tag="PL000_00715"
     CDS             complement(21373..22005)
                     /locus_tag="PL000_00715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC87443.1"
                     /translation="MSLILFDFDGVLADTLGDLLQFGQEVCDELGVRHIATQEDLNSL
                     EVMSFATYGIQLEVPAHLVDEFVRRCLSRFAEKKSPPAIFDGLAEVVRKLAKENVLAV
                     VTGNSTQNVNAFLAEHGILDCFRAVYGVDLPGSKVQKISLAREQFSAGMMRESVFMVG
                     DSLSDIRAAKEAGIQSVAVAWGHQSLDRLLSGDPDHVVRSVQELLTLFAV"
     gene            complement(22017..22247)
                     /locus_tag="PL000_00720"
     CDS             complement(22017..22247)
                     /locus_tag="PL000_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87444.1"
                     /translation="MRAFLIQSVVALVFGFVLGVIGLLVGAWFGGNFAASFEFNGVRG
                     YEATGQVGFILFALAGLIAGWKFMEKRTKKLR"
     gene            complement(22441..23043)
                     /locus_tag="PL000_00725"
     CDS             complement(22441..23043)
                     /locus_tag="PL000_00725"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HNC87445.1"
                     /translation="MEKSRLEAFSDGVFAIVITLLILDIRFPEVEYAQFSETLVSVVP
                     RILAYIMSFVIIGLYWVIHHNSMHAIKKTDRGFLWLNILLLLCVSFIPFPTSLVGRYP
                     FQAGPIVFYGFTLIACNAVGYIMLLYVYYHPHLAVAEFSKQYMRSHTPNYVIVNGAYL
                     GAILLANSFPLVSYLIYIAVVILLIVFLPKLDDGIKYHVE"
     gene            complement(23169..24050)
                     /locus_tag="PL000_00730"
     CDS             complement(23169..24050)
                     /locus_tag="PL000_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC87446.1"
                     /translation="MKTNQMMADNLPEHLNKLSPREIEVVDHVLQGKSNKQIALQLGI
                     TERTVEFHLKNVYTKLGIGSRMELAVKLWKSADDEVKASLRESTVAESVEIAENGNQP
                     MWAASVKRMLSVLKKEFAMTMKTILNDLGNAVQKHLLAFSLFYFIAAGLMTHYLVVGF
                     GLYFIPSYLLLGLVLGMSSLYLGLRWHGIKSGEVHVRPIWAVGFLALPFLAGLLDLTL
                     LATLAKAMGEVTVNLIGLSSRAVWMVADNGSPFLSLSRSITLDDIWLYGPVLYMSLLA
                     LAGRFYTIRILKRTDAV"
     gene            24128..25690
                     /gene="ftcD"
                     /locus_tag="PL000_00735"
     CDS             24128..25690
                     /gene="ftcD"
                     /locus_tag="PL000_00735"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNC87447.1"
                     /translation="MTNPLIECIPNFSEARRPEIIDQIVAAIQSVSEVKLLDRSSDLD
                     HNRTVLTFAGSPTGVEEAAFRAIKTASELIDLDQHTGAHPRIGATDVCPFVPLGGANM
                     ADCIAIAQRLGQRVGSELSIPVYLYEAAATRPERTNLENIRKGQYEGLKTEIETDPNR
                     APDFGPAKLGKAGATVIGARNPLIAFNVYLTTEDVEIAKKIAKAVRQSSGGLRYVKGL
                     GLLVDGRAQVSMNLTNFRETPIARVVEFIRREAQRYGVGIHHSELVGLIPEEALVDAA
                     VWYTQLDQFDKEQILESRLFDSGPQTTGSSSSPSHGLSTPQPASFIEQLAAPTPTPGG
                     GSAGAYAGAMGAGLVAMVAGLTIGKKKYADVEAEMQAIRVVAENLRQELTQAVDDDAA
                     SFEVLMATFKLPKDTDEQKATRNAAIAQATLNAAHIPLHVSEDAVKVMGLALKCAKHG
                     NVNAISDSMSGFAMARASITAAGCNVRININSLEDRTLGEKMLEELAELEKEADKLEA
                     EIRAVAKERGGI"
     gene            25776..27548
                     /locus_tag="PL000_00740"
     CDS             25776..27548
                     /locus_tag="PL000_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87448.1"
                     /translation="MNVTYNCAIQMQLEADFTPHQIKKENAMAGNGLIGKKVDRYEIL
                     EKIGEGGMATVYKAYDTRLEREVAIKFTKREAFPPDQLEMILKRFEREAKALGRLSHP
                     NIVGVIDYGEYDGAPYLVMTYLPGGTLKQKLGKPIPWREAVETIIPIARALHYVHSHN
                     IMNRDVKPSNILLTENGEAMLTDFGLVKLFEGQDTSQQLTDSGVGIGTPDYMAPEQWT
                     GETTALSDMYSLGVVLYEMITGRRPYISDTPAGILLQQVNEPLPLPKTYIPDLPANIE
                     AVLLKTLARNPKDRYANMNAFANDLQSILTGGQVTATAVATDILREQMTGKVSKKEIQ
                     EVKTQIEQKQAEQKQAEVPQRERTSPTSISNAGQPKSSKPMVSIALLFLGAIGLLVLC
                     GGCWLAYSFGPWAREAETPTALAVTSPEVPTATLEIEDTPTAKPSDTPVPQVTPTNTA
                     VPILLPQQWNGTYRQAGFNSTPISIVIEKMNGQTFTGKMVWTGGTNYRGAITVIEGEF
                     VTDFGDATEQAKWNKHLDYQNGDRDGIWIKWTETKFVSGGGYTLGGWYYGHVGKDGSM
                     TGIYFLNDQASSYANDSWKLKLVK"
     gene            complement(27614..29509)
                     /gene="acs"
                     /locus_tag="PL000_00745"
     CDS             complement(27614..29509)
                     /gene="acs"
                     /locus_tag="PL000_00745"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNC87449.1"
                     /translation="MAKKEMEGEVYYPSEDVIAQARLKDWDALAEKAGKDLQGFWADE
                     ASELEWFQKWDKVLDDSNKPFFKWFVGAKTNIVHNAVDRHLKTHRKNQLALIWESEDG
                     KQERTFSYYAMNREVSRMANIIKSMGVQKGERVTIYMGRVPEIVFAMLACAKIGAIHS
                     VVFGGFSVDALQGRIDDSQSKLVITCDGSYQNGKIVELKNIVDESIKRCPSVENVIVV
                     KRTGQPVAMEAGRDHWYHDLCALPIANGKCATEVLDAEDPLFILYTSGSTGKPKAILH
                     THGGYMVGTYSTIKYVFDVKDEDRWWCTADPGWITGHSYIVYGPMIAGATSMLFEGGP
                     TFPYPNRWWQVVERFGITIFYTAPTAIRGLMRFGEAWPNKHDLSSLRLLGSVGEPINP
                     EAWKWYYRVIGKEKCPIIDTWWQTETGAFQITPTPVVPLKPGSGTRPFFGQKAEIVDE
                     QGNPVPDDTEGYLTLLNPWPAMLRTIYGDDERYVNQYWSKYPGRYTTGDSAKRDKDGY
                     FWIIGRVDDVIKVSGHRLGTAEVESALVSHPAVAEAAAIGLSHEVKGQAIHTFVLLRS
                     GFSPSPELAEELRQHVAKHMGPIARPEDVKFLDKLPKTRSGKIMRRVLKARAQGLPEG
                     DISTLDE"
     gene            complement(29604..31517)
                     /locus_tag="PL000_00750"
     CDS             complement(29604..31517)
                     /locus_tag="PL000_00750"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNC87450.1"
                     /translation="MNFISPTVNRWLQEAKENPEAFWERAAKELHWFKTWDKVFEWNY
                     PNFKWFSGALTNLAYNALDHHVKRGWGGHTALVYMNERGERQLFTYAHMLYEVKRMAA
                     ALRGMGIKKGDRLTIYMPTCPEAIMLMLATVRIGAIHSVVFAGFGENALGDRIQASGS
                     RLVFTTDITYRKGKDVPLKGIVDGALAGTKTDVEHVILLKRGNDDVSMQDGRDITWQE
                     FIKYAEGQNGDCTPMEANEPAFILATSGTTAKPKLAIHTHGGYQVHIFSMARWVYGLK
                     PTDVWWATSDIGWIVGHSYIVYAPLITGCTTLAFEGALDHPHAEANWRIAIEEFAVSG
                     VFTSPTAVRLLMRYGDEPLSRVNHDYLERIFCAGEVLNAPAWDWLQNKMLGNKIPVID
                     HMWQTETGGPVFGNPYGIGMLPTKAGSATIPLPGIEVAVLTPEGEPCAPNEKGIMVIK
                     RPFPGLTPALWGEPERYGKDYWSKIPNVYYTGDSAHIDEDGYVWFAGRADEIIKIAGH
                     RIGTIEVETAFLKHPAVAESGVIGHPDELRGEVISAFVLLKNGKTPSAELKKELIDTV
                     RHELGPIAVIGELNFVSMLPKTRSGKIMRRVLKAVTLDKDPGDITTIEDEGSVTEARQ
                     AWQQMKDDISKGV"
     gene            complement(31607..33010)
                     /locus_tag="PL000_00755"
     CDS             complement(31607..33010)
                     /locus_tag="PL000_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC87451.1"
                     /translation="MSQVTANTPASGEQVEQGLPRIIFASAAGTMIEWYDFYIFGSLA
                     TVISSKFYQTGTPLGDLIAWLAAFAVGFIVRPFGAIVFGRVGDLIGRKYTFLVTMTLM
                     GLCTFAVGLLPTAETIGPAAGIILIALRILQGLALGGEYGGAATYVAEHAPNGRRGFM
                     TSWIQITATLGLFVSLGVILTTRSIMGEEAFAATGWRVPFLISILLVGLSLWIRYSLR
                     ESPLYVKLKEKKQVSKNPLKESFGNPYNLSFVILALFGATMGQGVVWYTGQFYALFYL
                     QKIFGVSLVDSNLIVGAALLAGTPLFVVVGHLSDKYGRKPFMMAGMILAVLTYLPIYQ
                     TMAKYAPFVGNAADKVVNPGYSPFMLGVLVFVQVVYVTLVYGPIAAFLVELFPTKIRY
                     TSMSLPYHIGNGVFGGLVPIIGLSLIQSTGNNFAGLWYPMVVAGICAVIGLWKLPETN
                     HIDISSDASVASLGANK"
     gene            complement(33439..33876)
                     /locus_tag="PL000_00760"
     CDS             complement(33439..33876)
                     /locus_tag="PL000_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC87452.1"
                     /translation="MYRKILVPLDGSKVAEGVLPHAKSLAYSEGAELILLTVGANPAL
                     DFAFSDPGLAQTAVDEQEEKSKAYISRIESELKADGFKTSTLLRVGSVAEVILGVAEE
                     LQVDVIAMSTHGRTGPARWLLGSIAERVVHNSNVPVLLIRARG"
     gene            complement(33894..34628)
                     /locus_tag="PL000_00765"
     CDS             complement(33894..34628)
                     /locus_tag="PL000_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC87453.1"
                     /translation="MRILVISDIHANFTAFEAVLKDAGSFDAVWCLGDVVGYGPEPNE
                     CINRLREFNPVCLAGNHDMAVAGRAAVWDFSKDAQEIIFWTRHRLSTQNLDWLNTLTD
                     RPLMTDFGITLVHGSPRDPVWEYMTDTGIAKSSFELIETPIGLNGHTHFPVIFRKPWD
                     DWRVLEEYPRINSPVKLTLDRMWINPGSVGQPRDEDPRAAYAMIDTEEMTYVYRRVLY
                     DVSAVQKLMKQEKFSNRLIRRLRFGQ"
     gene            34715..36073
                     /locus_tag="PL000_00770"
     CDS             34715..36073
                     /locus_tag="PL000_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87454.1"
                     /translation="MKKLFGRLSKAPQKDEAHAQTSFGVGTKIHQRYRLDAEIGRGGM
                     GTIYRAHDILNDRKVAVKIINAATANALSLDQFKREMEISTKLQHPHIVTTHEAGTVD
                     TDGKSLPFLVMELVQGTSLDKAGNLTYARIIDIARQICDALAYIHDQGFVYRDLKPSN
                     IILEPRGFQTFVKLLDFGLARPRGEAYLPNESSLAGTVFYLAPELVNGEPADVGSDLY
                     AFGAVLYEMITGRVPFSNVDEQTILTQHLNEQVTPPSQSRADVPSALESIVMRLLAKT
                     PQERFRSAQEVGFALEQISIAKTAAAIGNLPDAIPAAPQEPVTELIGLLTKSRLVTLL
                     SNDETLALAAASQTKDIFSDGIWLIQLTSIHDPARVMPTVMEVLNVSEIPNRPPVIAL
                     IEFLREKHLLLLLSHCGHLTGACVQLIKTILPACPEVRIVAVSTQALNLPEEICYKGT
                     AH"
     gene            complement(36084..37364)
                     /locus_tag="PL000_00775"
     CDS             complement(36084..37364)
                     /locus_tag="PL000_00775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC87455.1"
                     /translation="MKQHIYWHTTVQMPDASNLTPIPEKADVAIIGGGYTGLSAARTF
                     AKNNVHAVVLEAETIGWGASSRNGGMTLTGLKVAMQTAITRYGRDLARSLFQASIDSV
                     SLVEQIVQEENINCGFARTGHLLAANKPKHYEALKGEVDFMAKEFNHKVHLVSPKDLH
                     TEIGTDVYHGALVDEVSAGLNPAQYVAGLAAAAAKAGAILCARARVNRIRRNGTRFIV
                     ETERGSLEVDSVLVATSGYTGNVTRNLQRKIIPIGSFIIATERLSNELANELSPKNRM
                     IFDFKHFLNYFRLWDNRMIFGGRAAFFPENENTVAQSGEILRNEMLKVYPQLKDAKVE
                     YVWGGTLDFAFDQMTHVGEEDGMHYSLGYAGHGVAMATYLGKTTAEAMLKGNIKEHPF
                     AKFAFPAAPLGLYTGSPWFLPFAGMWYKILDWVE"
     assembly_gap    37374..37473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37474..37943)
                     /locus_tag="PL000_00780"
     CDS             complement(<37474..37943)
                     /locus_tag="PL000_00780"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC87456.1"
                     /translation="MDNSKIIYLHGLESTSQSGKARQFAQLFPGMITPDFTGSFEDRM
                     AQLAPILGDKKDWTIIGSSFGGLMGTVFTCEHPSQVRKLILLAPALLRDHFASYLDLE
                     PVSVPTVIIHGKEDDIVPLEPVRILAEKTFSNLQYILVEDGHRLHKAFDELNWK"
     gene            complement(37936..38922)
                     /locus_tag="PL000_00785"
     CDS             complement(37936..38922)
                     /locus_tag="PL000_00785"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC87457.1"
                     /translation="MPAIDLARLRKQANRLADFFFLPDEFMKHLREILDFYVNYTLRT
                     KENVAPGSNLKTYRTPPAVLTQIENELRAVATANPDHALNLADTLWDEGALETRLLAA
                     FLLGRIPPQEERLLPRLTAWTQQIRDPDVRSALLSTSLARMRRENPNQFFNLIREYLH
                     PARTRTWSNGIQALIPMIADTTTANLPPIFDLVEPIIEEAPSTLQNDLAELIVVLYRA
                     SSNETTFMLKHILANTENPMTVVTLRRISSAFPPPLQSALRDLLRPQPLSKPSPVEDA
                     ESSDFVTEETSEPVVKKPRRKLTTREPKPESAATDKKTVTRRKKKDESASNG"
     gene            complement(39009..40448)
                     /gene="proS"
                     /locus_tag="PL000_00790"
     CDS             complement(39009..40448)
                     /gene="proS"
                     /locus_tag="PL000_00790"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNC87458.1"
                     /translation="MAEEKLTTRAEDFSEWYNQLVIRADMADYAPVRGCMVVKPYGWA
                     LWENITSWLDAEFKKTGHVNAAFPTLIPMSFFEKEKDHVEGFAPELAVVTHGGGEELE
                     EKLAVRPTSETIIGHMYSKWVKSWRDLPILIVQWGSVLRWELRTKLFLRTAEFYWHEG
                     HTAHSSEAEAKEEMYRILDIYERFCLEEAAIPVFKGTKSDTERFAGAQITTSIEAMMW
                     DKRALQSGTSHYFGDNFAKAFEIQYLDKNNKLTYAQTTSWAISSRIIGAMIMVHGDDN
                     GLVLPPRLAPIQAVVVPIFKSDEEKAKVLEVADRIFAELKAAGVRVKFDDRDNVSSGF
                     KFNDWEMRGVPVRVEIGPKDVEKGTVALARRDRPGKEGKAFVSQANLDSTVKGLLDEI
                     QASLLKRATEYRDANIHNVTTFDELKEVVKDGWAFAYWCESRECEAKAKEETKATTRN
                     IPFDQPNEEGACVVCGKPSKRKVYFAKAY"
     gene            complement(40518..41048)
                     /locus_tag="PL000_00795"
     CDS             complement(40518..41048)
                     /locus_tag="PL000_00795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNC87459.1"
                     /translation="MNITVFGGAQPKEGSQAYEEARELGSLLAQRGHAVLTGGYMGTM
                     EAVSRGAHEAGGHVIGVTCIDIEEWRNSKPNQWVKEERRRQTLMERLMGLIEGCDAAM
                     ALSGGAGTLTEISLMWNLMIVESLPPRPLILVGSGWQSTFDQFFREFESYMPKHQREL
                     LYFAKDVKTAVTILQS"
     gene            complement(41115..41435)
                     /locus_tag="PL000_00800"
     CDS             complement(41115..41435)
                     /locus_tag="PL000_00800"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNC87460.1"
                     /translation="MDKELTIKELTRQMHDLVRSKGWYEADSKRPQTPRNLAVSLSIE
                     AAEILEHFQFGDEIKDRNELGSELADVTLYLLQLASVSGIDLEAAVLKKIEVNKTRKW
                     DVEE"
     gene            41744..42808
                     /locus_tag="PL000_00805"
     CDS             41744..42808
                     /locus_tag="PL000_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87461.1"
                     /translation="MPPILQFLIRRIFSAFISLIIITMVLYSGFMLTPPEARARIYLP
                     PGKGGERASEQYIENIIKENHLREPYLVQYFYWAKSLLQGSWGYSPTLNADVLPSLLH
                     RTPATLELALYSLLMLIPLGLASGLVSGWRPGSVFDSGFRGLAFLGTSTPSFIVSIIL
                     ISILYIQLDWLAPGRIDVKTELQMFRSGFVEYTGALTLDSLLNKRFDIFIIALKHLIM
                     PVFTLSIYHWATLGRITRASIMNERGKEYIVAARARGVKESKLIWKHALRAILAPSLT
                     AMALSAASIVTGLFVTEIIYGLTGISQIIVIAMSSYPDAPAALGFSVYSVIMVLGLML
                     ILDFIQATLDPRVREEVLKA"
     gene            42805..44193
                     /locus_tag="PL000_00810"
     CDS             42805..44193
                     /locus_tag="PL000_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC87462.1"
                     /translation="MRIVRYFFSRWQNWLGLLIVLMFTVVAISAPLLSPPDKNTQGSF
                     KKVGRSSDRTPHPPSETSILGTLPGQIDVYHTLVWGAREAMQFGLSVAIGAFIFGTLF
                     GSVAGYFGGFVNNIMMRIADAFLTFPVVAGVVLLQQLVAVTIESLGGIYWLNNDYFGR
                     VIDFQFTPPPFAVFLMKVDPILISLIIFSWMPVARIVNTMVLTLKNKEFIQSSRALGA
                     SPAWIIFRHLIPNSVGPAVVLAARDVGSSVILQATITFVGLGGASAWGILLSIGRNWI
                     IGPGGGLLRYWWVFLPVTIAIVLFGVGWNLLGDGLLEVLDPAANAYGGTSMIKRKQAK
                     GKKEAQSASDSKSIHHDSDSVAAQTATQGISVTLQNNPPVSHSQEKHPVLQVAREAIA
                     KKDFEHAIHAYSHLIKHRRNLNEIRQDMIEYVREHANEAVIWTLLGDVLTQEGKMEYA
                     EKAYEHARNLTQ"
     gene            44216..45460
                     /locus_tag="PL000_00815"
     CDS             44216..45460
                     /locus_tag="PL000_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87463.1"
                     /translation="MKLDLENERILSNRQLTLLRDISSLSAELMIPCHLVGGSVRDLL
                     LEQPIHDLDIVFEGDAISFGNILVNRFGGRLTAHQKFHTAIWHVPESLAEQIQTPFLD
                     LITARSETYARPGLLPNVTPSNMEDDLRRRDFTINAMAIRLDGEHFGELLDPLNGQQD
                     LKQGIIRVLHPRSFIDDPTRIFRAIRYESRYSFQLDPTTRELVNAEALDVFRMLSGER
                     LRHELDLIFEEEKALTMLQRLSEFDALRYIHPKLPAFNPTYADHLEMETGLEVPASRT
                     TLGYMFWLLDLSEDDIVSISRRLEFTSELTHSVWAASILRKSLPFLIGSSPSVWASAL
                     ENLPLLAVYAVYLETGEGALLSYISFWRHVKARTTGNDLKALGLPPGPRYGEILTQLR
                     AAWLDGKITTEKEEKELLNTLL"
     gene            45457..46224
                     /locus_tag="PL000_00820"
     CDS             45457..46224
                     /locus_tag="PL000_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC87464.1"
                     /translation="MNLQPYLDFATSLAYRAGRITMGYYNTGVRADMKENNEPVTIAD
                     HEAEKFIRAEIEKNYPGHAIIGEEFGHSQGNGSPFRWTIDPIDGTKSFIRSVPLYGVL
                     IGLEIEGRISVGAAYYPPLDEMLCAADGLGCWWNSRRAHVSSVDEISKACLVTSDFER
                     LEEKDPTLRERFARKKALLRTWGDAYGYMLVATGRAEVCIDPFLDIWDYGPYPVILRE
                     AGGYFGTWSGEEGHTPGDALACNAALKQEVITLMQGS"
     gene            46274..46855
                     /locus_tag="PL000_00825"
     CDS             46274..46855
                     /locus_tag="PL000_00825"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNC87465.1"
                     /translation="MSNTERLPYRLREPFLATTELALFRALQQMVGARYLVCPKVALN
                     DIFYIVRPNENVHFFNKFFRKHVDFLLCDPQTLAPAFGVEIVRPIAKNETRESDRFIE
                     ELFIDAGLPLVHVPSSETYDPSDIVSLFQLAVTKVGGTAALRLDTSSDSVPLCPVCGK
                     MMVLRINRDGPRSGKKYYGCMDSPRCAGLVPID"
     gene            complement(46888..47790)
                     /locus_tag="PL000_00830"
     CDS             complement(46888..47790)
                     /locus_tag="PL000_00830"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC87466.1"
                     /translation="MKIAVTGAFSYSGKYIAGRLLERGEEVFTLTNHPDRPDPFDGKV
                     KTFPLDFNDQEGLIRNLSGMDVLVNTYWIRFDKGDNTQPRAVENTKILVEAAAKAGVK
                     RIVHISITNPSAKSHLPYFWGKAANEKAVMESGMSYAILRPTVLFGKEDILINNIAWL
                     LRRLPVFGLPGDGSYKLSPVYVDDLAEMAVDAVYKKENYIWDAVGPDEFTFKEMVELI
                     GKSIGVKKLLLPFPPRLALLAAQFMSLFVKDVMLTPEEVDGLMANLLVSKEAPRCRTH
                     LSDWLERHRASVGSQYASELARHF"
     gene            complement(47800..48330)
                     /locus_tag="PL000_00835"
     CDS             complement(47800..48330)
                     /locus_tag="PL000_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC87467.1"
                     /translation="MPIKLSQLKQDFTEGLSQISRFWGFPKGMGAIFAVLYLSQNPLS
                     LDEIVEATGLTKGAVSTEVRTLARMGLVHRSSKLADRKDYYMAEDDFYKSIRSILKER
                     QNNEFDSAIRSVGDALSKLEAGDVTGDKAEVDFTYKRIQALQEFFNAIDSLTRAVVKL
                     ESLGFTNVKTILSVLK"
     gene            complement(48453..49049)
                     /locus_tag="PL000_00840"
     CDS             complement(48453..49049)
                     /locus_tag="PL000_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87468.1"
                     /translation="MTKFQKWITTALVVSVAAGVFTLQNNLQPPSSENVQSTDSTLTP
                     EIAAPVESLTPTLAYEGCGFMWATHDAPELTEKIDLAVRKMNDKAWANANFFGEDCVY
                     ADGRTTFGVMETDVFIHLPVDDLLDNEFFGDWIAQVMAMIVQIPKEEFQNRYGFVEFW
                     FEASETDHLVVRVPIQKYLEVGQGVSGVEMLQLFIETP"
     gene            complement(49209..50090)
                     /locus_tag="PL000_00845"
     CDS             complement(49209..50090)
                     /locus_tag="PL000_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNC87469.1"
                     /translation="MKKLTITILLLMLLLSACTPPVSPTEAPLATEEPSQVGPAVDIP
                     ANPEPLSTATEAPVAREAKISEVQNEVLARTLSTSDLAPASVGTSILPTGGVETGTDG
                     RARLDLLPEGTIVRVGPNSSFTVPEIVQENGQPKTVIELLFGKVYILLNGGSLEVETP
                     SGVASVRGSLMRVEYDAETKRIKASCLEGHCALKNENGDEVELVAGEYTYIEGNFPPI
                     DPLLIDSSEIEEWLAEIPELGDFLNPLPDPKDYPDVEDGNDNGNGSNDNGNGNDNNNN
                     DDDDDDDDDNNNGGYPN"
     gene            complement(50133..51323)
                     /locus_tag="PL000_00850"
     CDS             complement(50133..51323)
                     /locus_tag="PL000_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC87470.1"
                     /translation="MTDFLNEARSLFSYTQTLRRDFHLHPELGFREIRTSGIVAKELE
                     ALGLEVTKGVGKTGVVGLLEGAKPGPTLLLRFDMDALPIHEETGAEYASQNAGVMHAC
                     GHDGHTAIGLTVAKLLNQHKDKLAGTVKFCFQPSEEGNNGEEVGGAEMMMRDGVLDGP
                     KVDMTLSLHLWNEKPLGWVNVAKGPVMAGAEEFKIKVIGKGGHGAIPDLAVDPVVCAA
                     QIVSASQSIVSRNVGPLETAVISFTVINGGTAFNVIPQEVMMQGTIRTFDPQVRTKVL
                     QRFDEVVRGVASAMGCQVELNVKQLTPALINAERVAASVQATAQRLLPNLHHDLSPYL
                     TMGAEDMAYMQAKVDGCYFFIGSNNHERHLDYGHHHPKFDFDEQALITGSALMAAAAA
                     DILK"
     gene            complement(51337..51903)
                     /locus_tag="PL000_00855"
     CDS             complement(51337..51903)
                     /locus_tag="PL000_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC87471.1"
                     /translation="MNIVNVGYQSTNYYLIDILGGKLLVDCGWPGTLPQFSAELKRKG
                     IRLQEIKYLLVTHFHPDHAGMTQELKAIGIKHILLDVQVDFVFQMTEFFKTKPHPYRP
                     ITTDDSIRLKVEESREFLLKLGLSGEIIHTPGHSDDSVSLFLDEGFVFTGDLSPIFVL
                     PESDTISRASWDRIYQHQIKTVFPGHGK"
     gene            complement(51945..52673)
                     /locus_tag="PL000_00860"
     CDS             complement(51945..52673)
                     /locus_tag="PL000_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC87472.1"
                     /translation="MTLQPENWTSPQNILVILAHPDDPEFFCGATLARWARDGHKITY
                     LLLTCGDKGFNPTTNPEMTPEKLCGLRHGEQNNAAKIIGANSVHFMDLPDGYLIPDLN
                     LRRDIVREIRRHKPDILVTCDPQTLFATYGINHPDHRACGQVVLDAVFPAAGNLVYFP
                     DLLAQGFEPHMPKEVWCSLTNQPNTSVDVTDTWDIKMQAILEHKTQVQDPKALIERFK
                     TRRTEDSTEENPRYEEKFKVVKYG"
     gene            complement(52728..53717)
                     /gene="ricT"
                     /locus_tag="PL000_00865"
     CDS             complement(52728..53717)
                     /gene="ricT"
                     /locus_tag="PL000_00865"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNC87473.1"
                     /translation="MWKPENGLWLAIPYGIIVPMQTNIIGVRFTKIGKIYHFDSSTLP
                     DVKQGEHVIVDTTRGKHLGEVVQVLTETPPQPEGGWKAVERRATPRDLLLQQSWQSRQ
                     TEAMINCRARASELHLRDVKIVTAEYNYDGSRLTFLFSTESEDKVDLKSLKKDMQQIY
                     PNAQIEMRQIGPRDVAKFLGGMGACGIETRCCSKFLTDFSPISIKMAKEQGISLTPNE
                     ITGMCGRLRCCLIYEYEQYVEARKTLPKRNKRVVTPKGEGKVIDVVPMSDKVVVLIES
                     GVDRPQQMTFAREEIEPWDELEALRRKSQAPCDRHEGGGCTCGKAPRNNNNKN"
     gene            53913..54005
                     /gene="kdpF"
                     /locus_tag="PL000_00870"
     CDS             53913..54005
                     /gene="kdpF"
                     /locus_tag="PL000_00870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HNC87474.1"
                     /translation="MNTLYAIVGIITLLLFGYLVLALIKPEWFG"
     gene            54002..55720
                     /gene="kdpA"
                     /locus_tag="PL000_00875"
     CDS             54002..55720
                     /gene="kdpA"
                     /locus_tag="PL000_00875"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HNC87475.1"
                     /translation="MNTNNLLQLMFFMGTLLLLVKPLGSYMARVYQNERIFLDRVLGP
                     VERLIYRASGIRPTEDMDWKSYAAALLVFNMLGLFVVYAQQRLQLALPFNPQGFSAVT
                     PDSAWNTAVSFASNTNWQSYGGETTMSHFTQMLSLTVQNFVSAATGMAVLVALIRGIA
                     RHSGKGIGNFWVDLTRSTLYILLPLSFILAMLLVSQGVVQTFNGYRTVSPLQSAEQVI
                     AVGPAASQIAIKQLGTNGGGFFNANSAHPFENPTPFSNFLEMLSILLIPAALCYTFGQ
                     MVGDLRQGWALLTVMTIIFLVLVGITIWSELGGNPALPSLGIDQIQSASNPGGNMEGK
                     EVRFGIVNSALWATATTAASNGSVNAMHDSFMPLSGLVPMWLMQLGEIIYGGVGSGLY
                     GMLAFVIVAVFISGLMVGRTPEYLGKKIEAYEMKMASLIILIPVTVVLTGTALAVSIP
                     DGRAAIYNPTAHGFSEVLYAFSSAGNNNGSAFAGLSANSPFYNLSLGIAMLISRYWLA
                     VPVLAIAGSLVRKKKIPASAGTLPTHTPLFMAWLIGVITIVGALSFIPALALGPIVEY
                     LTIIGG"
     gene            55744..57828
                     /gene="kdpB"
                     /locus_tag="PL000_00880"
     CDS             55744..57828
                     /gene="kdpB"
                     /locus_tag="PL000_00880"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HNC87476.1"
                     /translation="MSIQTRKHNSFRREIYQRAVTEAFIKLNPRLMIRNPVMFVVEAG
                     SALTTILWVQALLGKGEASSGFIGTIAIWLWFTVLFANFSEAVAEGRGKAQAESLRKA
                     KQDTPAKKLKKTQLDGATSEHEVISSTLLRKNDLYLVVTGDILPADGEIEAGIASVDE
                     SAVTGESAPVVREAGGDRSAVTGGTRLLSDWLIVRVSADPGQGFLDRMINMVEGAKRQ
                     KTPNEIALNILLAGFTLIFLVVCATLLPYSIYSVEAAGKGTAITITVLIALLVCLIPT
                     TIGGLLSAIGIAGMDRMIQRNVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVEFI
                     PAKGVTASELAEAAQLASLADETPEGRSIVVLAKEKFGLRGRTMNAPAGMHFVPFTAQ
                     TRMSGVDFDGISIRKGAPDTMLSMADFNGHPFPADLRSSVDSIARMGGTPLVVTRNTK
                     ALGVIYLKDIVKGGIKERFAQLRKMGIKTVMITGDNPLTAAAIAAEAGVDDFLAQATP
                     EAKLKLIREYQSGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQPAREAANMVDLDSN
                     PTKLIEIVEIGKQLLMTRGALTTFSIANDVSKYFAIIPAAFASTYPVLGALNFMKLAT
                     PQSAILSAVIFNALIIIALIPIALRGVPYRAKPADQLLRDNLLVYGLGGLIAPFIFIK
                     LIDLILVWIGVA"
     gene            57836..58009
                     /locus_tag="PL000_00885"
     CDS             57836..58009
                     /locus_tag="PL000_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87477.1"
                     /translation="MKRNHHLLPYFIYTSAILGFTAAIAPDKLNIPTNFRPWVFLASI
                     AWAVAVLSGVFTA"
     gene            58006..58569
                     /gene="kdpC"
                     /locus_tag="PL000_00890"
     CDS             58006..58569
                     /gene="kdpC"
                     /locus_tag="PL000_00890"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009305207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HNC87478.1"
                     /translation="MKTHLRPAFTILTLLTVLTGLVYPFLVTGLAQVFFPQQANGSLI
                     SVNGTVYGSELIGQSFDDPKYFWARPSAAGYNAAASSGSNYGPTNPALMDAIQARIAA
                     LQTADPENTLPIPVDLVTASGSGLDPHISVAAALYQVHRVAAARGLSEAEVRSLVEQN
                     TEGRQFGFLGEPRVNVLMLNLALDGIQ"
     gene            58575..61241
                     /locus_tag="PL000_00895"
     CDS             58575..61241
                     /locus_tag="PL000_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HNC87479.1"
                     /translation="MQQRPDPDELLKHIQPEEHGRGKLKMFLGYAAGVGKTYAMLEAA
                     HQRKAQGVDVVIGYVETHKRADTDELVEGLEVLPRKQVNYHNVTLPEMDVDRILARKP
                     ALVLVDEFAHTNAPGSRHPKRFQDVEEILNAGIDVYTTLNIQHLESLNDIVAQVTGVI
                     VRETVPDRVIDEASEIEVIDLPPDELLTRLKEGKVYIPEQASRAIDKFFRKGNLTALR
                     EMSLRRAAERVDDQMRSYMQTRAISGPWAAGERLLVCVSPSPLSERLIRSARRLADEL
                     NAEWITVYVETPQFAAMSQEKRDRVAKILQLAEDLGARSYTLSSNGSIESTAQAVIDF
                     AHKNNITKIIAGKPLRPRWQEFLRGSLVDKLIHKSGDIDIYVVTSMDVPAFPADENPL
                     KLHSPLYKYFLSMLLVGFATIIGFVIGGRIEPTNLVMVYLLAVVVASVYLGRGPAVLA
                     SLLGVLAFDFLFVTPYFTFAVSDTQYIITFIALFLVGMVISQLAARASEQAEAARQRE
                     AETAELYDLSRDLAISAELESILAVLQKHVEKIFSRTVAVLLPTENRLTSYILSTGLE
                     LSDEEAAVADWVYRHGEAAGRNTNTLPAAQLRYLPLKTARGVVGVLGVGKPNTIERDL
                     TPAQRRLMEAFANQCAQAIERVRLAEEARQIKLLQSAEKLQNALLNSISHDLRTPLVS
                     ITGALTTLEEQASELESPAHRSLVETAREEADRLNRLVGNLLDMTRLEAGALKVKCEP
                     SDIQELIGSAISQMESRLDRRRIEVQISDALPLVPLDFVLIVHVLNNLIDNALKYSPE
                     GSPLEINADLVKDEIQISVTDNGIGIPHEDLERVFDKFYRVQRPENVTGTGLGLAICK
                     GIIEAHGGRIWAKNNAVGGSVFTIALPMEEAK"
     gene            61238..61912
                     /locus_tag="PL000_00900"
     CDS             61238..61912
                     /locus_tag="PL000_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87480.1"
                     /translation="MTRILVVDDERPIRRFLKASLGNEYEVVEAVDGEDAIRVTATEK
                     PDLVILDLGLPDMDGVEVTRRLREWTQIPIIIVSVREQETDKISALDAGADDYLTKPF
                     GAGELMARIRVAMRHASSPETQAVFESGDLLVNLSDRIVKVAGEEISLTPNEYDILRA
                     LVQHAGRVMTHHQLLRTVWGNAYENEAHLLRVNMSNLRRKIEADPARPRHIITEPGVG
                     YRLKIA"
     gene            complement(62187..63260)
                     /locus_tag="PL000_00905"
     CDS             complement(62187..63260)
                     /locus_tag="PL000_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNC87481.1"
                     /translation="MTLSKESVLAALGKVQEPELHQDLVTLNMIRNLEISGDKVSFTV
                     MLTTPACPLRGRIEKEVREAVMAVDGVKTIDLKMDSDVPNDGRMRGLVSMPIRNAIAI
                     GSGKGGVGKSTVSVNIAVALAQSGARVGLMDADIYGPNTPTMLGVDKLPPPSGQKLIP
                     AEAYGIKMISMGLLVKPGQPLIWRGPMLNSAIRQFLGDVVWGELDYLIIDLPPGTGDA
                     ALSLAQALPLSGSVVVTLPQLVSLEDASRGLNMFKQLEVPILGVVENMSYLDLPDGTR
                     MDLFGSGGGEELAKATDTTFLGKIPIDQNVRIGGDTGKPIVAAHPESAVAKSIQEIAQ
                     KIAAKASVAAMSANNALPINIVE"
     gene            complement(63345..63959)
                     /locus_tag="PL000_00910"
     CDS             complement(63345..63959)
                     /locus_tag="PL000_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC87482.1"
                     /translation="MYGKGILKGLSVTWKRFWDTYTEDISWMLRGKKRYNTVEGVAHR
                     SSKDTKGIFTVQYPEEQLIQPEEFRYVPFLVYDEGADGKKEVRCTSCGICSKVCPPQC
                     IWIVRTNDPATGRPVPVPTNFYIDMDICMNCGFCAEYCPFDAIKMDHDFDIASYDRNV
                     YNLEKLLKPAKYYAEIRPENYTREEEARKAKEAAKAAKAEAASA"
     gene            complement(63974..65719)
                     /locus_tag="PL000_00915"
     CDS             complement(63974..65719)
                     /locus_tag="PL000_00915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC87483.1"
                     /translation="MATAIQTSTVDLVARFPGKVTADTRPGYSGFIVNKDNLVEVATA
                     IRDEFGFDLLTAATGADYIAENKMEMVYHAYKTTGGPGLVFKVQVERVDPVEIPSLIN
                     IWPGVDFQEREAWDLLGIKFTGHPDLRRILMWEGYEGHPLRKDWKEPFFEEEAKPFKS
                     RWPDGQHTFAENKNPFRDNLKFPADFDPEKFEIVNEDGLYASLERHTMKNEEGSLNTD
                     HIVVNMGPHHPSTHGVLRVAVTLDGETIIGLKPVMGYLHRNHDKIGERNTYLQVIPYT
                     DRLDYFNSMSNNFGYVTTIEKLMKIQVAERAEYIRVIMAELSRVQNHLIFIGMLMNDL
                     GTMYTPSLYAFEERELVLDIFEAVSGARMMCNYFRFGGVVRDIPDYVMQKIKDLVFER
                     LPAKTDEMERFLNENEILVARLKGIHVLNAEDAIKYSVTGPVLRAAGVPYDLRRADPY
                     SIYDRFDFDVAVRQNGDMMDNYLIRFDEIRQSLRILEQALKQIPQGPINSQKPQYQVR
                     VPAGEAYGRIESPKGELGFYVVSNGKPNPYRYHVRPASFVNLQAVESMCIGTKIADFV
                     ALLAMLDIVMGELDR"
     gene            complement(65869..66357)
                     /locus_tag="PL000_00920"
                     /pseudo
     CDS             complement(65869..66357)
                     /locus_tag="PL000_00920"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(66347..66556)
                     /gene="iscX"
                     /locus_tag="PL000_00925"
     CDS             complement(66347..66556)
                     /gene="iscX"
                     /locus_tag="PL000_00925"
                     /inference="COORDINATES: protein motif:HMM:NF016284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNC87484.1"
                     /translation="MTLNWESTYAIALELRRQHPEVNIEDVSLQQIYQWTIQLSEFED
                     DLALVNDDILYAIYQDWFEENIHGK"
     gene            66733..67308
                     /locus_tag="PL000_00930"
     CDS             66733..67308
                     /locus_tag="PL000_00930"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HNC87485.1"
                     /translation="MITARHKVLAHLKKTRAASAREIARALKMSAPNVRHHLSVLCSD
                     GRVESTEVNNREGRGRPEKLYSLSQAALGDNLSALTKALLTESGPRLNIEALANHILN
                     SGQFANLPITKRLVLLIEKINEMHYQARWEASAEGPRVILGRCPYAKIIAGHPELCKM
                     DAAILKNALGNSVEQTSKMENLHGVCIFAMR"
     gene            67330..67884
                     /locus_tag="PL000_00935"
     CDS             67330..67884
                     /locus_tag="PL000_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC87486.1"
                     /translation="MTPNHLTKEELEAGLQHITQSPKDNGVVEMIVRRPEVDGREILE
                     SAELNLTEGLVGDNWKTRGSTATPDGSAHPEAQINIMNSRAVALLAQEKDRWALAGDQ
                     FFIDLDLGVENLPAGTRLALGSAILEVTAKPHTGCDKFAARYGRAAALFVNSTEGKKL
                     HLRGINAKVVQAGTVRVGDAVKKI"
     gene            67968..69005
                     /locus_tag="PL000_00940"
     CDS             67968..69005
                     /locus_tag="PL000_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC87487.1"
                     /translation="MKAIRFIGANQPLQMQEIPIPEIGERDILVRVKAAGICHSDAHY
                     RAGISPVKPVPLTLGHEVAGVVEKVGAQVGNLKIGERVCLHYNISCGDCYHCSTGNDQ
                     FCEKVLMLGHYTNGGYAEYISVPARNAIRLPDEIPFEQGATLMCASATAFHALRKSRL
                     KAGETVAIFGAGGLGQSAIQLAKAFGAVEVYAVDINDDKLNLAAQYGAIPVNGNKVNA
                     VEEIKRLTHGKGVDVAIEMIGLPLTMKQALQCAGVMARVVIVGLSNKPLEIQTYTELL
                     GNEVELIGSNDHLLQELPLLVDLARRKVLDTSKIVTRTVPLDANAINATLDSLEKFSG
                     DVRTVIVPASD"
     gene            69045..69296
                     /locus_tag="PL000_00945"
     CDS             69045..69296
                     /locus_tag="PL000_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNC87488.1"
                     /translation="MNIPDRIISILELRRNTGERYSRMWIAHNGIVYDVTDCPKWRTG
                     MHEFLHFPGQDLSGELTDAPHEEEVFKHDCVKIVGRLES"
     gene            69371..71092
                     /locus_tag="PL000_00950"
     CDS             69371..71092
                     /locus_tag="PL000_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNC87489.1"
                     /translation="MTAHKWWQTAVFYQIYPRSFADGNGDGIGDFKGATEKLDYLASL
                     GVDAVWLSPHFPSPNWDCGYDISDYCNVAPEYGTLDDFKTFLNEAHKRNIRVILDLVL
                     NHTSDEHAWFLESKSSRDNPKADWYVWVDTPPNNWQSCFDGEAWTYVPERGQYYYHYF
                     MKQQPDLNWHNPEVKKAMWDAARFWLDMGVDGYRLDAIGTIFEDPQLTPHNVPMNLAE
                     LRRFSELANTPKEKKLKDKYWYEMFKHQYGKPGVHELMKELRAILEEYEGDRMLVGED
                     DDIAYMGNGWDELQLIFNFPLMRTDQITPAHIRKNQKDRLAALEKLPVETGWPCNTLG
                     NHDCSRIHTRYGDKIHDAELARLNAALVLTLKGTAFLYNGEEIGMTDFMLSDISHVKD
                     TMGSWYYHTIVTEMGVHPEEAMLRTAEMTRDKNRTPMQWSNSPNAGFSPSGVNTWLPV
                     NPNYKDGINVYDQDKNPSSLLHYYKHLLRVRKGSPALQHGEYLPLHNTSREYFAFLRR
                     SENQTVLVVLNYSDKVFELDFSRTKEIKDSNLRILFTSAERRGGSFAPRFKVGAFEVF
                     IAETERG"
     gene            71085..71300
                     /locus_tag="PL000_00955"
     CDS             71085..71300
                     /locus_tag="PL000_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87490.1"
                     /translation="MDKEKAKNSKKFVALCGRIFRDADPKYFPSAQYRGRTLRFCTES
                     CLGAFLADPDIFYKVHRNSEKNKDAKP"
     gene            71287..71934
                     /locus_tag="PL000_00960"
     CDS             71287..71934
                     /locus_tag="PL000_00960"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNC87491.1"
                     /translation="MPNLNLWKAGDHILLRGVYNGSPTYMQSARVVQDTPEETALLIW
                     PGAECMVPSGYLHHAHDRSMWDRWAETTTNTLKLEKYIWHTNRFLILLEPEKYFSTIY
                     IWEAAKDEFVGYYINFQLPYRRSHLGFDTLDLDLDIVIEPDFQWSWKDMDEYQQGIEA
                     GGIHADWVNEIERAKQEVFTRLEKRGYPFNSDWLNWRPNPNWTAPSLPENWDVID"
     gene            71953..72726
                     /gene="fdhD"
                     /locus_tag="PL000_00965"
     CDS             71953..72726
                     /gene="fdhD"
                     /locus_tag="PL000_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNC87492.1"
                     /translation="MNAPQKSIHYEQYEFQKWKSFDAETIVEMPVSLTVNGKVWISFM
                     CTPVNLEALAVGFLFNEGIIQEMDEVEDVRVCEHGDNVDVWLSHAAEQPTSWRRTSGC
                     TGGVTAVDLLAKPNVSLSGNSFTVRPDAIGQLVEMLFESQSLYRETGGVHTSALSDGE
                     KILIAAEDIGRHNTLDKIAGLCLMNDIWPENRILITTGRISSEMLQKAAQINAPILIS
                     RTSPSSLSIEMAERYGITLIGYARKHRFNVYTNGERVGL"
     gene            72741..73067
                     /locus_tag="PL000_00970"
     CDS             72741..73067
                     /locus_tag="PL000_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87493.1"
                     /translation="MAKKDRSNGTPISFQIPSSGGKGPRIPIPTNQQKWKYQSGYMAR
                     PTPKEWIAAVIAFFVIVGIGFLLSVESWALTVAFCVFAMIIPVSFIQREMFFRKSRKD
                     QAKPRR"
     gene            73311..73667
                     /locus_tag="PL000_00975"
     CDS             73311..73667
                     /locus_tag="PL000_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87494.1"
                     /translation="MNLNQVIELVGGTTLTTEADLNINIQGGYGADLMSDVLAASQPN
                     AVLLTGLTNPQVVRTAQMADFRAIIFVRGKQPQAETLELAEQENMPLITSPLGMFELC
                     GRLHKAGLPSFESNVS"
     gene            73657..74664
                     /locus_tag="PL000_00980"
     CDS             73657..74664
                     /locus_tag="PL000_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC87495.1"
                     /translation="MCPNRIRTEQEIHGRHGRPITDQDAHEINRVEELSYDLKIHEVM
                     SKNLQTASPDISLSDVLKILRINRISGVPVVQDEKLVGVISIEDIVRALQNNDLTETV
                     GQYMTRELVTVASFDSIVKAMQTFAEKHVGRLPVVDEEKKLVGMITKGDITRGILVAL
                     QKDYKAEEVRRYRASHLFEDIISDRTTLVLRYNIKAQDFTHGGNASSHIKRALLRLGA
                     DAQIARRCGIAVYEAEMNLIIHTTHGGILKLEVEPHRITMSTTDDGPGIPDVEQVLQP
                     GYSTATDQVREMGFGAGMGLVNIKRCVDNMELESTVGKGTKLIMRIHVPAENFNARKE
                     G"
     gene            74671..75012
                     /locus_tag="PL000_00985"
     CDS             74671..75012
                     /locus_tag="PL000_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87496.1"
                     /translation="MNLQQIIERLNLNLLTDPRDFETIHPEGGYTSDLLSCVMAGAKS
                     KYIWVTLQAHLNIVAVAALLDVAAIIITEDAQPDAATITKANEQGVILLSTSMPSFEV
                     DGKLWEIGLRN"
     gene            75025..75765
                     /locus_tag="PL000_00990"
     CDS             75025..75765
                     /locus_tag="PL000_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC87497.1"
                     /translation="MKNFRADLHVHTVLSPCAEVEMIPPLIVQEALERQIDIIAITDH
                     NASANVAAVQKAAHGTALHVFGGMEVQTREDVHLLCLFDHTEALANWQALVDSALPNL
                     KNNAEFLGEQFIVDEEGNFIRSEPRMLLTSTHFSIDEIFNKVNALGGLVIPAHVDRNS
                     YGLFPTLGLLSPNWPILSLEISRHISPEKAASLYPFIKNTPLIQSGDVHRLDEFLGKT
                     IFTIDQPSLAEIRMAFRNESQRTVNIEP"
     gene            75882..76394
                     /locus_tag="PL000_00995"
     CDS             75882..76394
                     /locus_tag="PL000_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC87498.1"
                     /translation="MTQSLKEIPATDPLSIAEQKQIIDTAILENKDRPGSVMLVLNEV
                     QGQIGHVSPSMQAYIARQLGVPLGQVHGVVTFYSFFKTKPRGKHTIKFCLGTACYVAG
                     VPQLIEKAKQMLNVELGETTPDGQITLEECRCVGACSQAPVVVVDEEVQGRLRPNKFP
                     QVLKKVQKPS"
     gene            76391..76960
                     /locus_tag="PL000_01000"
     CDS             76391..76960
                     /locus_tag="PL000_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87499.1"
                     /translation="MREIALHLMDIAENSVAAGGRNIRIAVHEDLQGDLLTATVSDDG
                     RGMDEETARQVMDPFYTTRTTRKVGLGIPLLKLAAEISGGRLSLVTEAGKGTKIEACF
                     RHSHIDRMPLGDLASTFLALLVSYPNIHWVFDYKTTDKNGNDDNFEFDDAELKNELGD
                     MSLTEPEILKFVRGLIEEGIQASALQTNF"
     gene            76973..77341
                     /locus_tag="PL000_01005"
     CDS             76973..77341
                     /locus_tag="PL000_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HNC87500.1"
                     /translation="MPAIKSLEELKRVREEALQKKKMKAAPGNVQVIVAMGTCGIAAG
                     ARDTMKSVLNYIETNNLSGITVTQTGCIGMCEQEPIVQVVIGDQPKVVYGKVNAEVAG
                     QIMKQHVQNGQPVKDYVLPS"
     gene            77392..79176
                     /locus_tag="PL000_01010"
     CDS             77392..79176
                     /locus_tag="PL000_01010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC87501.1"
                     /translation="MAFYRSHVLVCVDPECLAKGAHEVVDALQDEFVAQGLIDEIQVL
                     ETSRIGGCSDGPEMMVYPEGVHYHGMTADDIPFLVEEHFLKGRIVERFREVTKPVVDE
                     ELGPLRPKEVRVVLRNCGEIDPNNIEDYLATDGYQALGKVLFEMTPEQVIEEVSNSGL
                     RGRGGAGFPAGRKWGLTRQVQDNPKYMVCNADEGDPGAFMNRRVLEGDPHSVIEGMII
                     GAYAVGASYGYIYCRAEYPVAVETLNIAIKQAREFGFLGNNILGTDFNFDLEVRVGAG
                     AFVCGEETALMASIMGMRGEPRPRPPFPSVKGVWGKPSNNNNVETYSNVPQIILKGAA
                     WYASMGTERSKGTKTFAVGGNVVKPGLIEVPMGITLREIIFDVAGGIKDGKKYKAVQT
                     GGPMGGCLTEEHLDLPLDYEALTQAGSMMGSGGLVVMDETSCMVDIARFFMDFTQAES
                     CGKCTPCRVGTRRILELLQKICDGKGQDGDIEKLENLCHEVARNSLCGLGQGAPNPVL
                     STLKHFRHEYEAHIYEKRCPAKVCRPLIHYEIQAPTCTGCTVCARNCPVEAISGERRQ
                     AHHIDPDTCIRCGICMQVCNFNAIAVES"
     gene            79210..79707
                     /gene="nuoE"
                     /locus_tag="PL000_01015"
     CDS             79210..79707
                     /gene="nuoE"
                     /locus_tag="PL000_01015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNC87502.1"
                     /translation="MTAPTIERDVVSVVQAAIEKHGNGRDAMIPVLSEINRQFGYVPR
                     EAIREVRKQLHDPARQVHIAESQLYTLTSFYDMLSTKPRGRHVIKFCENAPCHVVGGK
                     AIWDGLREKLNLENGETTPDNKWTLVTTSCLGLCSVGPVLLIDDDMYGNVTPEQITEI
                     LEKYE"
     gene            79719..81359
                     /gene="nuoF"
                     /locus_tag="PL000_01020"
     CDS             79719..81359
                     /gene="nuoF"
                     /locus_tag="PL000_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC87503.1"
                     /translation="MIKKRAMILVSNDPESIRLGANEIVRHLNEALVTYGLQDEVDVS
                     TVSDVNHSNILPLVVVYPEAVVYGPVKAADVRYLVEEHLYKGRVVNDLLAPPKMLTGQ
                     IGWLRGHKGYNPAEQRIVLERAGRIDPESIEEYITANGYEALGKVLGEMTPEGVIDLV
                     EKSGLQGRGGAGFPTGRKWKFVRAAAGSKKYIVCNADESEPGTFKDRIVLEGDPHVIL
                     EAMAIAAYALGADEGYIYIRGEYGLAYRRLEKAILQAREFGFLGKNIFGTNFNFDIHI
                     HAGAGAYVCGEETALLESLEGKRGEPRSRPPYPVTRGLWNKPTSVNNVETLANVPAII
                     KNGAEWYRSIGTPSSPGTKVYTIMGNVNFSGVIEVPMGVTLREVINIYAKGMKPGSTL
                     KLAQTGGSSGSIIPAVLQDTPMDFESFRKAGVSLGSGALLICDQNTCIVDLVKVLLQF
                     FRFESCGKCTPCRVGTQRAYEIVDRISKGQGRLEELDTLLVLANEMERASNCGLGQTA
                     TVPIRDMLKHFRTEVEAHIRSGSCPTGVCEMAQEIEKV"
     gene            81412..83391
                     /locus_tag="PL000_01025"
     CDS             81412..83391
                     /locus_tag="PL000_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC87504.1"
                     /translation="MAKITINEKQIEVPDGTTVLRAAEMAGIHIPRLCDHKELTPYGG
                     CRLCIVEVEGARVPMASCTLPVNNGMVVRTNTPALKQSREFILSMLFSERNHFCPFCQ
                     VSGGDCELQNSAYHEEMTHWPIQPGWSNFAVDTTHPYFILDNNRCILCRRCVRACGEL
                     AGNFTLSVAERGAASIIVADTDVPLGESTCIKCGSCVQVCPTGALIDRVSAYQGHDKH
                     LEEIKSVCSGCSVGCSIKIMVRDNRIVRIEGDWDGKVNRGVLCEHGRYDPVTESRKRI
                     TTPLVRKNGKLEPVSWDEAMGAVAGKLMENKNSIAAIASTRLSTETLSAFKELFSEKF
                     QSKLVTSVEEGVPTASVTRFAQTHEAFEGKLDVLRNADTVMCIGANLHRSHMVAGFMF
                     KRNLSKGTRLITIDPESSELDYMANIALKTQPGSDLALIRGLQAVIVQEELGRAPLSL
                     PDAGQLIEKAVSASGVSLEELRQTGRLLARSISPVILFGKGVTAQRDETIVEELHRLA
                     VLIGAVDSERHGLLSIKGEANSLAAALLSMDSAFELNGEKVVYAAIGDDYISKSLVER
                     VSKAPHLVVQASYESKLTEQADVVLPVTIWAEQEGHYINLDGRIQESHKALVPPETVR
                     ENLAILNELAMRTNTTIKTDWQKAILARRSSVALN"
     gene            83414..83956
                     /locus_tag="PL000_01030"
     CDS             83414..83956
                     /locus_tag="PL000_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNC87505.1"
                     /translation="MNKPKIASDWMCGCAGCHMSLLDMDERILQIVELADLRATPITD
                     LKEPDESGVDVGVLEGGINNSANEEVAHKMRQRAKILVALGDCAVFGGVPAMRNLCGV
                     EAALRRAYVEAESNEPNSQIPNDPELATMTKARAIHEVVPVDYSVPGCPPDADTIFHV
                     LAELAQGRKPEIKDDLLHWH"
     gene            84022..85449
                     /locus_tag="PL000_01035"
     CDS             84022..85449
                     /locus_tag="PL000_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNC87506.1"
                     /translation="MSTQKITIEPVTRIEGHAKVTIHMKADNTVDHAYMHVNEFRGFE
                     KFCEGRLYFEMPQITPRICGICPVSHHLAAAKAGDALTGQTPPRPANLLREMMHMGQM
                     IQSHGMHFFELAGPDLLLGFDASPEIRSVVGLIGANPELTVKAVQLRKFGQEIIKTLG
                     GRKLHPVFAVPGGVNKALTMAERDSILSGVDTAVATLQAGLQIMKDWAQKNMEDINKF
                     AVFPTGYFGLTTPENGLELYDGNIRLISREGVEMERFPVANYLDVIAEHVEPWSYLKF
                     PYYKKMGYPDGVYRVGPLGRLNLAEKIDTPLADRELKIFKSLNNGKPVENTLYYHYAR
                     LIEALFAAERVRVLCEDPDILSTDILNTRRNYTGHGVGVLEAPRGTLIHDYTADENGK
                     LLKVNLIVSTGHNNWAMTHAVDSVAKTYVKGPDVHEGMLNRVEAAIRAYDPCLSCSTH
                     AVGQMPIQLDIVAADGTLVKTLKRD"
     gene            85540..85986
                     /locus_tag="PL000_01040"
     CDS             85540..85986
                     /locus_tag="PL000_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC87507.1"
                     /translation="MTTVRKIVEEKAETKVHTIAADATVLDALQVMNEANTGAVLVTE
                     NEKIIGIFTERDYAREGEVKGKFAKDTIVRDVMTSQMVMIKLETTLQECAELMRQYRV
                     RHLPVLDNDRVVGMVSIRRLAEALLQEQKGTIVELENYILGTGYGE"
     gene            86058..86540
                     /locus_tag="PL000_01045"
     CDS             86058..86540
                     /locus_tag="PL000_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNC87508.1"
                     /translation="MKKTLIIGYGNPDREDDGVAWHILRALTLKLGLPAPASYEDEFP
                     QNGAFEFAFHLQLTPEMAEDIAAFEQVCFVDAHTGSIPEEVRLIEVESDFQRSPFTHH
                     LTPQSLVSICESLYQRKPEAVLLSVRGYQFLFSRQLSAQTEALVPQAVRLIWNWLQMS
                     "
     gene            86632..87078
                     /locus_tag="PL000_01050"
     CDS             86632..87078
                     /locus_tag="PL000_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC87509.1"
                     /translation="MTTVRKLLEAKETSKNYSIASTDTVLKAIRLMAELHIGALLVTD
                     GGKIVGIYTERDYLYKGENEGRAAKDTLLKDVMVSSMITVTKDTSVEQCMALMKQYNI
                     RHLPVVEHDQLVGLVSMRDVMFAALENKESEIRGLENYIMGSGFQS"
     gene            87187..88809
                     /locus_tag="PL000_01055"
     CDS             87187..88809
                     /locus_tag="PL000_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC87510.1"
                     /translation="MSVQKKSSFKFLFRSIILHLLAAILVSCAPATATPAAHPTYDPF
                     LPVSEGTFALSPNIPTVTITSTPVPPTATREPTPTRVPLTIILPTAVPTDQPIVTPTP
                     DAERVLPTPRQDEAQYVVQFGDTLGGIAFMYGISVEDLMLSNNLTDANMLDVGMTLNV
                     PAPNPSEGGTSFKVIPDSEMVYGPGSAYFNIQEFIESQNGYLEIYEQDVSGFILSGYE
                     IVWIVARNYSINPRLLLALLEYQSGWVTNPEPFNTAYPMGLADEGHYGLYRQLTWAAN
                     TLNQGYYLWKANAISTWVLNDGTVVPVDPTINAGTAAVQYFFAQLDDRPVWEQDVSGT
                     GLLLSYYVFFGNPFDFAIEPLVPAYVSQPTMQLPFSEGETWYFTGGPHGGWDYGSAWA
                     ALDFAPPGDNGGCGVSPLWVRAVADGLILRASNGALIQDLDNDGYEQTGWVILYMHVA
                     EEDRVEPGEYVYAGEPIGHASCEGGISNATHLHLARKYNGEWIAADGPLPFNLDGWIS
                     SGGGREYDGYLSRNGITIEALDSANELNQVYR"
     gene            88848..90476
                     /locus_tag="PL000_01060"
     CDS             88848..90476
                     /locus_tag="PL000_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC87511.1"
                     /translation="MSRTLAILIVLSLVLSACMSSSPSLWGVPQTPTPNTVDLASAPI
                     DPFAIQDHPIDFPTSTVPPEIATENAYTPTPTITPNSSTPVIEPTYTLAYDAAPFLYY
                     AQSGDMLSAVANRFGVDESEITSDADLTKTTLIDPGTLLVIPNRITEPMTPYVQIMPD
                     AELIFSYTAVDFDVKEYVQQSGGYLSKFKDYLGSTGWIDGDAAIERLATENSINPRLI
                     LAMLEFESRWVRGQPVDELHIDYPMGFNDYHYKGMSVQLTWAINNLAIGYYSWRAGTL
                     THLEFPDGTTMRIDPRLNAGTVAIQYLFSRSHSPSQWEQIINPSSGFPAMFNEMFGDP
                     WARADAVNPIFPSALNQPPLVLPFEPDVEWSMTGGPHNAWGQINPKIYGPKNSVFAAI
                     DFAPATDHGGCDPTPTWVTAAAPGLVVRSSNGAVMVDLDGDGYEQTGWNLLYMHVGAK
                     DRVAVGTWLEVNDRIGHASCEGGVSTGTHLHFARKYNGEWVTADGPIPFIMSGWRVVA
                     GEKAYEGKLVRGEEVVIADPVGQKWSNIFRKENE"
     gene            90469..91953
                     /locus_tag="PL000_01065"
     CDS             90469..91953
                     /locus_tag="PL000_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC87512.1"
                     /translation="MNNLSRIKQKLSSAFLVFAFLLSACNLEASVEPQVPTPTSDIAP
                     YSFAPTALPTRPTYKPGELVDYIAQNGDTLPALAARFNTTVEEIFQANPFIPREATTM
                     PPGMPMKIPIYFLALWSSPFQIIPDHAFVDGPTVIGFNTSAFVASQPGWLKDYRVYAG
                     GDWRSGAEMVDYVATNYSVNPRLLLAILEYQGGGLTAAEPPVKKNLLGFTRRYWENPY
                     LQLVIAANTLNNGYYGWRTGEMIEFEDTNEIIIRPDPWQNAASVSIQYYFSRIYDGQK
                     YAVATGPQGLFLTYEKFFGNPWQDSTIIIPGSLAQPEFQLPFPSDQTWYYTGGPHTGW
                     GTGEPLAGVDFAPPSDKSGCFVADDKNFAVAMADGLVVRSGVEGVLLDLDKDGNERTG
                     WVIFYLHLATKDRAPLGAELKTGDKIGYPSCEGGRATGSHVHIARKYNGEWIMADGIL
                     PFNLNGWITHNGTREYLGTLTKGSIIVTACECGDAFTAISGSFP"
     gene            complement(92055..92522)
                     /locus_tag="PL000_01070"
     CDS             complement(92055..92522)
                     /locus_tag="PL000_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC87513.1"
                     /translation="MDVLLNWKGKMSFEGAGDSGFVQKMDSDSSVGGDDNGARPMEFI
                     ALGLAGCTAMDVISILQKKQQPVVDFKVQIHTERANEHPKVFTEAVIEYVVTGNGVDE
                     AAVLRAIELSAEKYCPAQAMLSKAFPIRMVYKILGDGGETIKEGEYFSKRDSV"
     gene            complement(92526..93104)
                     /locus_tag="PL000_01075"
     CDS             complement(92526..93104)
                     /locus_tag="PL000_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC87514.1"
                     /translation="MTDSPLIESPQPPTARPFAAMLIGAGLIAIGIMFYMLLSQQDVS
                     SQDFSAVPVKVDYPAPKLDLVDLSGKSVSLDDYLGSVVLVNLWATWCPPCREEMPTLQ
                     TFYEKYKEDGFVLLGVDQEETAAVVQPFVEEFGLTFPVWLDLDYLAQREFNTMNLPSS
                     YVIDRSGTVRLMWIGGISKRNLEKYVPDLIAE"
     gene            complement(93153..93488)
                     /locus_tag="PL000_01080"
     CDS             complement(93153..93488)
                     /locus_tag="PL000_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87515.1"
                     /translation="MSNQQSSIKYHKLDDIFPFFKKCWCPWVILCEISNFPNLRLFNV
                     VMTEISKLSALERLLLGLDAPPKLETEPLRKDALCPQCHLGRLDYNGLLQLECPACGY
                     VNGEGGGCT"
     gene            93506..93973
                     /locus_tag="PL000_01085"
     CDS             93506..93973
                     /locus_tag="PL000_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC87516.1"
                     /translation="MLELLPNIPLFENLDTAQIALLKPLFEQFNCAANTVVFEQGEPA
                     SYLYLLLKGEMLIRFKPYDAPPITLTRLRDGDVFGWSAVLGSTHYTSSLVSETQIESI
                     RIRGSHLLMLVRDHPETGKIIMDRLANVVSSRWKNARTQVQALLNSTNQNIKE"
     gene            93979..94224
                     /locus_tag="PL000_01090"
     CDS             93979..94224
                     /locus_tag="PL000_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HNC87517.1"
                     /translation="MVATQIQTQEMQLRGLLEKLSAYVEQFHGGTVEFVSFDGKALRV
                     HLGGACLNCPLLPSTLHGWVAGTVHQFFPDIEVIEEK"
     gene            complement(94583..95350)
                     /locus_tag="PL000_01095"
     CDS             complement(94583..95350)
                     /locus_tag="PL000_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87518.1"
                     /translation="MKRFDPNIVFGFLLLVGGGLALAQTMGILENASRLFWGGLFLSI
                     GLIFLTLLFGGHWWAAIPGFTLLGLGALVLLPKSLDEIGGALFLGGIGLGFWYVFLSA
                     REERWWAIIPACVLSALAVMIVAAQRYEELGGAIFLGGIGLAFLFVFFTERNSRWWAL
                     IPAGVLVTLATVTVAAQRFGEFQTAGIFFFGLAITFLLVAMLTGMRWAYWPALGLGVM
                     GVLGIISLLELANYLWAVVMIAAGGFLLFRYFSNRNV"
     gene            complement(95362..96159)
                     /locus_tag="PL000_01100"
     CDS             complement(95362..96159)
                     /locus_tag="PL000_01100"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNC87519.1"
                     /translation="MRRSQLFWGSALLLWGLFLLFDQMGLRLPNGNSISSLFWPLALL
                     LAGGWVLLGVFMRGKIEDENASIDLQGASSAHVRINHGAGELKLHGGASPSEVARGTF
                     AGGLNQKSTRNGDSLDVRMKPAKEFVDFPFFGGGNQLDWDVALNANLPMALTMNLGAN
                     KAALDLRDLTITDLKLETGASETKLTLPSRGRFRADLDLGAASLEVIVPAGVSARIRA
                     TVGAVDLKVDESRFPRSGGYYQSQDYDTAANAVDLSVDAGAASVKIS"
     gene            complement(96161..96694)
                     /locus_tag="PL000_01105"
     CDS             complement(96161..96694)
                     /locus_tag="PL000_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87520.1"
                     /translation="MNKQGRTQLALGILLILLGAWFLLDQTMPAFHTFFERYTEWPIN
                     LVLIGGAIFIVGLVLGSPGLSVPASIVAGIGGIFYFQEVTRSYDSWSYMWTLIPGFVG
                     VGTVLQGILGENTAHNLKRGLNMMVVSAVLFLVFSAFFGKWNLLGDYGAPILLILLGI
                     YVLGRGLYRSFRNRSEE"
     gene            complement(96795..97553)
                     /locus_tag="PL000_01110"
     CDS             complement(96795..97553)
                     /locus_tag="PL000_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNC87521.1"
                     /translation="MSLDDPILTPVELLAYRRAAKSLQNFPPPETVIFVPQKSLADYV
                     LRKYWVSQIKGFLGGFYLLKKSKGRIAFSTGFGIGAPVVAGLTDEFVALGVRRFALIG
                     MAGGLQENLSAGSLILSTGAIRGEGVTRHYLPEEESVEASQEMAEGVGAILKKGNRPY
                     FHGLTWTTDAPFRELRRDVLAYQQRGVLAVDMEAAAMYAVAKANGRPALAAFSISDSL
                     AGGRWSMSGDLRPAQAGLSVLFEAVLEFALAHSA"
     gene            complement(97543..98715)
                     /locus_tag="PL000_01115"
     CDS             complement(97543..98715)
                     /locus_tag="PL000_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNC87522.1"
                     /translation="MSIRRNSFWLLLARLTAQALAVLFTAVIARKLGVNNFGQFSFIA
                     SLILIGNTFSNFGTDTFLVREIARVGRVTEVAGQALGLQLLLSVVYCFAMLAFRSGPL
                     LFYSLALFPLAFFSVNNALLRALNRMDLFWLLSLLNGFIQVLSAFFSSNVLALCIFLF
                     VGQALLSTVSYLVCRLSLPNFSLFPIQSSSPIFKLIFPFAVLTILLVLIQRLGVLAVS
                     ALLNNEAVGLFSSITRVVDGLKLGHYAILGALLPALSGGVSGSWNSFRKAFILLMTVS
                     LLFVVGLTFLANPLMLFLYGNDFVQASSLLALLGWSLLPYTISSFISYGLIARGYESD
                     VVKSALFSLLGYAAVNLVLISIAGLRGAIWSVLIGECFQAVVFMFFYFRRFQANES"
     gene            complement(98748..99569)
                     /locus_tag="PL000_01120"
     CDS             complement(98748..99569)
                     /locus_tag="PL000_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC87523.1"
                     /translation="MPLNNPAFQTVAEAYDRNADKYDEFIENNPNLMRMRQRVYQFVT
                     ARLPKGSRILDLACGTGTDAVWFAQNGYSVFGVDISGEMLERAKQKAKDLNLQDRLSF
                     EQLSYTDLKEASIGRFDLTFSNFGGLNCVSDMRLVADSVRPLMNQDGRVVWALMPPFC
                     LWESAMLLRGKFKLATRRFKRKSTVFKEGLNYPVFYYTPKEVADAWGSEYRLESIEAL
                     SVITPPATNKDFAIKRPRLFETLSKLDDALASKFPFKYWGDFTIVSLAAVSDASR"
BASE COUNT        25625 a        26582 c        24136 g        23233 t
ORIGIN      
        1 ggcagcagga gcctcggtgg cggcaggggc ttcggttgca gcaggtgctt cagtggcttc
       61 tgcgggggct tctgtagcct caacagcagg ggcctcagtg gcggcggttc cgccacaggc
      121 ggaaagcaca aggctggcaa acacgaacaa aaccaaaatc ttatttacaa gctttttcat
      181 tctctctctc cttataagtt gttatgacga caattggaaa catgacaaaa gcagattagt
      241 tgcgagtcag cctcctttca tatgttatct attgtcgcac tgaaccgctg gcagggcgcc
      301 agcttttgag tgcagaatta tatgtacagg caagaataaa cgtcaactat ttatttccga
      361 ataagacaaa gggtataatc gaaaaaaacg attttttgga gacaaaccaa tgacagaatt
      421 gccgattcac cctctcagcg atgaacacaa aatgctccgc gatgccgccc gcgattttgc
      481 tcaaaaagag atcgcgccca ttgcggcaga atttgatgag agtggagaat tcccgtacgc
      541 caccatcaga aaaatgggcg aactgggctt catgggcatt gaaatccctg aggaatacgg
      601 cggcgccggc atggacgcct tggcatacgt ccttgctctt gaggaaatct gcaaagtgga
      661 tgcctcacat ggggtcatca tgtcggtgaa taattcgctg tactgtcatg gcattctcaa
      721 attcggcacc gaggaacaaa agaaaaattt catcaccccc attgcaacgg gcaaggccat
      781 tggcgcctac tcccttaccg aaccccaaag cgggtcagat gcgggcgcca tgaaaagccg
      841 cgccacacgc gacggcgatt tttacatttt gaatggacgc aaatcctggg tgacaagcgg
      901 ccctgtggct aattatttcg tcgttttcat gatgaccgac cctgataaaa atcagaaagg
      961 cgtgacagct ttcctggtgg atggaaacac acccgggctg actcgcggca aaaaggaacc
     1021 caaacttgga atccgcgctt ctgcgaccag tgagctgatc ttcgaaaatt gcaggatccc
     1081 cgctgaaaat agactcggcg aggagggcga aggcttcaag atcgccatga ccgttttgga
     1141 cgcggggcgc attggaattg cgactcaggc ccttgggatt gctgaatctg cctacgaagc
     1201 agcgaggcag tattcgggtc agcgcgaagc gtttggtcag cctattgcag cctttcaggg
     1261 aacaggattc aaaatcgcag atatgaagac ccgtatcgaa gcatcccgcc tgctgatcta
     1321 taatgcggcc attgccaagg aaaattcaaa aaccaatcac gggcggtatt cgcttgaagc
     1381 atccatggcg aaattgttcg cctcagaaac agccatgttc gtcacccatc aggcaattca
     1441 gatccacggc ggcatggggt acagcaagga actacccgtg gagcgttatt tccgcgacgc
     1501 aaaaatcacc gaaatctacg aaggcacaag cgaaattcaa cggctcgtca tttcacgcat
     1561 ggaaacaggc ctgcgatagg agtggcattg atgtcttccg ggcaaatatc actgatcgcc
     1621 gttatcatca ttacgatcta cctggattgg gttttgatga aatggctgaa gaaaagaaaa
     1681 aaagagaacg ttgagaaaaa gcccgttcaa aaatgggaca ttcaactttc aagtttttat
     1741 aaacccatac tccaatggat ctccaaattt aattttgaac tgcctctgat cgcactctgg
     1801 gcactttggg ttggtcgcgt ttacctaaac ttcgaccgca acatggtacc cggctatggc
     1861 aacgacgatt tcctgctttc catctatcct tactttccgt tcatccgtgt gctgcaatgc
     1921 ggagattgct ttttctggaa tggcctgctg aacggtgggt cacccacatt cggcgatagc
     1981 attggtgcgt tgttacaccc ggttatggcc ggtatcatgc tggttttcgg ggtcaccaat
     2041 ggaatcaaag ttgccatgct ggcctcgctc attatgggcg gatggagtct gtggtggctg
     2101 gcaaaggtgc tggatctgaa aatggtaacc cgtctatggg tttcagcatt ggctgtggtc
     2161 gggggccatc tggcgggacc gctttttagc ggcgacgtgg gcatcatctt ctcgacagca
     2221 gccgccagtt tgacggttgc accagcagtt gcgcttgggt tacatggcag gcggcgcgat
     2281 gccattctat ttggattgat ggttgctcaa gccattctat ccggacaagg ctatttacaa
     2341 ctggcgctgt ttgccggtgt ggtgccagcc ctgctgattt tcggaatcaa cagatatctg
     2401 caaatcggca aaatatggaa ggaacttgcc ctggctctgg tcattgccat attggtctcc
     2461 agtgtggtgt tgctgccctg gttcacgttt ggctctcaaa tctcacgccc caccgagccg
     2521 cgttcagacc agccattgga atacatgcca atgaatctgg tcgttcgcga tatgaatttc
     2581 ttcatgacag aaacgctcgg aaagatcgcc gcccccaatc tatacgtcat gtacattggc
     2641 tggatcccca tcttattggc gatcgcggcc tggcgcttca ccccacgtga aaatttccgt
     2701 ctatatttat tcttcgcaat cgcaattgcg ttgatctttt tcctcggaag cggcgcccct
     2761 ttgcgttggg cgaaagactg gctgccgggg tatctgcgcg atatccgcaa ccccaacttt
     2821 ggggtcgggc tggccgttac cctcattttt ggactggccg caatcggcct cgatcagatt
     2881 cttaacttga agtggcccgt tgtttcagtc tacaataatg tcacgaagaa gagtcacccc
     2941 attgatttcc gctggttgct tgcccccatt ctattatggt caatcacatc ggtttacgaa
     3001 tttaatagcc agtttcgtgt cttgcatgaa tggccaaaag aactggtcag agccgcagaa
     3061 tccatcccta cacaaccagc acaatgggtg cgccccgtat atggaaattg gacttacgtg
     3121 gcagcagccg tccgtcataa tgtgaagatc acagaaacct tccgcatctg ggaaatgatc
     3181 ggccgtccaa acccacccat gagtctggat tcaggcaatg accccgccat ggtcaacgac
     3241 cccaattatc taaaaacagt tgagggtatt tcctttgtgc tttaccccga aaacttttac
     3301 gcctttgtag aaaccgatac aggtcgaatt ccatgcgaag ccattgccga tggcgggcat
     3361 attcaggtga aatgcaataa cgcagaaacc ggggatctgg tcgtttacga aaacgctttg
     3421 caaggctggc gcgtgtggcg ggacggaaaa tcaaccccat tagaggaagg tcaatggctc
     3481 aaggtttcag cccccgcagg aaaccatacc tacaaattca gatattggcc aaccgatgta
     3541 ttgggcggtt tcgtccttac cttgatcggc cttgggttat cctattggtt ttggcagaag
     3601 gaacaaccca gccctgttac tacttcccct gagagagaat ctttttaatg aacacgttaa
     3661 gtaatcttac caaatccata accagggctt ggggaaggct tctttcctcg ccaaccacat
     3721 ggtacaccgt cctcattgaa tggtccatta ttgtctgtgc tgcgtatttt tttacagcac
     3781 ctgtacttct ggattttgac cccaatcaac tacaacagac cggtgaacac aacgagagtt
     3841 ccacccgccc attactggcc gaaatcgggt tattgcaaca cggtgaaatc cccttgtgga
     3901 accactacat gcaaacaggt tttccacacg ccggagactt gctggggcac ttttggtcgc
     3961 caatcgcaac catccctgtc attttatggg gcggtatcaa tgggatgaag gtctcgatct
     4021 ttatttcctt tgtcctggcc gggctgggac agtggtacct cgcccaagtc agcggactca
     4081 aaggcatatt ccgcttatgg agcagtttga ccttcatgtt ttccggcggc cttgcatttt
     4141 tatggcgtct tggctggtat gagcttttgg tcggtatggt ctggttccca tggacctttg
     4201 cagccttctg gcaggccctc aaaaaacgag atatcacctc catggcatgg acagcctttt
     4261 gcgttgccat gatcttgttc actggcggcg gctattatcc cttttacttg atcgccagtg
     4321 caggtacgat gttgatcgtt gcactgatcg ccgcccaacc agaagaacgc ttcatccaga
     4381 tcaaacgcgc tctgtccatc gctgtgttta gtgcagggtt gatcgcagtc atggctcttc
     4441 caatttacga tgggtatcgc ctcatcgtac gggaatcagg agatgacctt gtacagcagg
     4501 gctcacagcc tgtttcgtac gcagtgatga actatgtcat ttcacaaggt gagtggttca
     4561 acaccgaaat attggggact gccggtggct ggaactggtt ttacacaggg ccgctgatgc
     4621 tgggtgcgct gttgtttctc ccgttgggat tccagcacag tagaaaaagg tccatgcttg
     4681 cggcaatgct ggcaatttcc attgtcttgt tgttatgggt ggccaatcgc tatcccccag
     4741 tcaaatattt atacgattac gtcccatttc tgtatcgact gcgctttccc aaccgcctgc
     4801 tgatattggc ggctagtccg ctgctcattc tcagtgcagc cagcttgcag ggattgatga
     4861 tcgtgttgcg taagcaattc aaaaattaca ggatatcctt ctcctaccag aatgatggac
     4921 ttgaaaaaac cactcgctta cgcggcctca ccatcatcat ctttatcgtc atcctgttct
     4981 tttcgttgaa agatgtttat ctaaccaatc acagctttgg tttcgtttca ttccccatcg
     5041 agcccaaatc tttgcaggct ctgcgttacc tgaagaacaa agatgaaggc atgtactaca
     5101 tcaacctcgg cggcggcgcc ttgtactgga gttggactcc cgcggcatac attgtccaac
     5161 ttccagtgat caactttaac tacaaccaac acttattaaa ccgggatagt cagaacctgc
     5221 ccgaagcgcc ttttgttgcc actcccaaat atcaattctc gccgacgctt gaaaacccgc
     5281 caggcgcaac cttgctcaag gattttgatg gtgttggttt atggtatttt gccgatgcgt
     5341 tgccgtttgc attcaccgcc ccgggcaacg aacttgtcaa tggcaccaaa cttgacccca
     5401 cacacatcac tgaagcatct gcccattacg acggtccaaa ccgcgtggtg gtgacaacag
     5461 cagaagacca cacaggggat gtggtggtgg cattggtcaa caactacccc ggctggaagg
     5521 ttctccgtga tggtgaacct gttccgctta ccccggccaa tgaatacctt gccgcccctg
     5581 ccctgcccgg caaacacact tacacattta tctttgaccc gccactccat aagatcggcc
     5641 tgaccattac cctgctgaca atattcgccc tgctcttttt tgtccaccgt gaacaattgg
     5701 actgacccca ggcgcatttc agcaggatga aattcacctc aacgacaatg gatatccatt
     5761 cacaccagcc aatacctcga aaatattggc tggtgtttgc tttaattctg gtcgttctcg
     5821 gccaagcctt gatgagggaa ccacagccat tcccatattt cgacacactg gctgacatgc
     5881 taaaaaactg gaacgatgat gcatatcttg ccctgagcaa tcctttcaac acattgatcg
     5941 gcctcgctct cattctactt ggcaccatag tctcggcttt atcaacaaga accgccccag
     6001 ctgaggatta ccccatcatg ggcaccatgc actgggaaaa atggaaacgg tacaaacact
     6061 gggtcattct caatttaatc gtgtacgggt tcatcatggt ccagcttgcc cttcacaaag
     6121 attcggtcac attcttttgg ggttggctgg cattcctgct tgtgttcacc atcctttttt
     6181 gggaaaacga gcataaaccc atggaatgga tcaccctcac cgatgcagca tggatactgg
     6241 gtctattcac acttgccaca gccatgggag cctacctgct caatgacctc cccgctggct
     6301 ggatcgccga tgaaggtcca ttctgggttg cggcacgaaa catcgccatc ggtaaaaccg
     6361 atccgtcctt tttcgacaca ggggtattta cctttccgat cgcaagctcg cttctacagg
     6421 gatggatcat gcgttgggca ggcatcaaca tgtggggatg gcgcttcgct tctgtactac
     6481 ccgcggcagg gtcagtcatc ccactttatt tgcttacccg tgagctattt gaccgccgca
     6541 cagccatcgc ggcaagtttt atgatgatcg ccaatccata cttccttgcc tttgcccggc
     6601 ttggatacaa taacattcag gctttgatac ctgtcaccct gtgcctttat ttcctaacac
     6661 ttgggctgaa gaaaaacagc ctctattatt tctggctggc tggtttggct gcaggggttg
     6721 gtttctacac ttatttcgcc gcctggctgg gacttgtcgc cgcaattctt atcatccttg
     6781 cttttacact catttcacgc cagtcatttc ggagcggcat cacttcgcta ttggtcgtct
     6841 caacagcagc atgcctcgtc atcctgccga gagttttgta cggcgcaagc ggtgaaaacg
     6901 aaaaatcatt aacctacaaa atctgggaaa ctggccccat caacctgttt tatggaaaac
     6961 tggtcttcgg cgatgaacgc atggcggaag ccgatatctt caaccttgag ggggttgatc
     7021 ttttctacga cgcttcctta tacaaactcg tgttcacgcg cggatttatc cgcaccacag
     7081 ctgtgttatt cgacccgatc ggttacaacg accaccccat cgtttttggt ctggctggcc
     7141 caggctcatc catttttttt gcattggggt ttggcatggc attggttgac ctaaaaaaac
     7201 tacgccatct gattatgctt attttttttc tggcaggatt cttttttctg ggtgtgcttg
     7261 cctcaatccc accacgccca acgcacatgg tggcgattct gcctgccatt gcgatgatct
     7321 ctgccagtgg attaactaat ttcacaacaa ccgtgctgaa tacaatcaaa gaacgtagaa
     7381 tcatgctgac cacggctggc attttgtcca tcatcgtgtt gatgggttgt gtacaatatt
     7441 tttttctact ccctttcgcc tacgcgccgc caaatcaaaa cgactatatt gcatggctta
     7501 cacgccaaat tcaagaaccc atccacctgt acatcattga caaccaggtc gagcccgttc
     7561 aagacagcct aagcccacac acaaccacaa tgttgtcatc cgcagaaata agtgtgaacc
     7621 ctgaacaagt atcaacatgg aaaaattttg tggcctttat tagtccgcaa agcggggcgg
     7681 actatgctga agaactggcc gcccaaatcc ccggagcgga cgtacaagcg gcatacgccc
     7741 cgggccagcg cctacgcgga tatgtcatca gcgaccttcc cctaaaaacc agtatggaca
     7801 tcagcctcca gcatggtata acagaccttt ggaattcacc agcccgttcg attctgctca
     7861 tttgcggagt gggctccata tttctctttg caatgcagtg gttgactggc aaaacatcga
     7921 ataaaatgag tgaaacatta tgaaaacaat tctcttccac aaacacggcg gaccggaagt
     7981 attggaatac accgatttcc ccatgcctga gccgaaaaat ggcgaagtcc tagtccgctt
     8041 gaaggctgct gccttgaacc gtatggatgt gatggttcgc aatggctggc ccgggttgaa
     8101 acttgaactt ccacatatca acggcgcaga tggtgctggt gaagtggcag gcctgggcga
     8161 aaatacaaaa ggattcaaga tcggcgaacg cgtcgtcgtt aacgccaacc ttggatgtgg
     8221 cgattgcgag ttctgtctga aagggcagga caacatgtgt ctcaactggc atttgcttgg
     8281 cgagagtgtg cgtggcacct acactgaata tatctgccta cccgcccgcc agctatacaa
     8341 actgcccgag gatttcaacc ttcatcaggc tgctgcggcg gctttggtgt accagaccgc
     8401 ctggcattcg atggtcaagc gcgggggagt aaaggttggg gaaacagtgg caattgtcgg
     8461 cgcgggtgga ggcgtaaatt ccgccagtat ccaggttgca aaatatcttg gcgcacgagt
     8521 catcgtcatt ggatcaaatt cgaaaaaatt aaaagaagcc gaagcgctgg gtgcggatat
     8581 tttgattgac cgttcaaaag aggaagattg gtcgaaagcc gtttttattg ccacccaacg
     8641 gcgcggtgtg gatgcggttg tagataatgt cggcacaacc ttcccactga gcctgcgaat
     8701 tttacgcaag ggtggaaggc tgctgacagt gggcaacagc ggcgcgcctc gttttgaaat
     8761 tgacaaccgt tatatttttg ccaaacatct ttcgatcatc ggctcaacca tgagcacact
     8821 tgccgatttc gccgaagtga tggatctggt cgtggatggc cgcctccaac ccatcattga
     8881 caggactttc ccgctcaagg aagccgccgc cgcacaggaa cgcctgtgga acggtgaaaa
     8941 ctttggcaag atcacccttg atattccata aatgaatttt ctaaaacgtc tcccttcctt
     9001 tgaagccgtg ttgatcgcgg ttattctagg cattcatatc tacgcggcat tttcagatgc
     9061 atacaacctg ccgaattcat ggttcacacg tgatgatgcc tactactatt tcaaggttgc
     9121 acagaacatc accgagggat atggcatcac gttcgatggc atcaacctga caaacggcta
     9181 tcaccctttg tggatgctca tctgcattcc gattttctcc cttgcccgtt ttgacttgat
     9241 cctgccgctg cgggtgatgc ttttggtaat ggcaggcttc aatgcggcca cagcgatctt
     9301 gatatacagg ttggtaaaaa gcaatctctc aaatgcagtg gccatgctgg cggcttcgtt
     9361 ttgggcgttc aatttctaca ttcacaataa cgtgtatgaa ttcgggttgg aaacaccgct
     9421 ggcggcattc gcggttgtgt tgttcatcta caaactttcg cagcttgaaa aagactggcg
     9481 aacccaggct gtgacaaaaa gacaatggct caatctggca ggtgttgcaa ccctcgtcat
     9541 gctcagccgc ctcgacctgg tcttcctcgc cattctcggc ggcttttgga tcatcctgcg
     9601 cggcaagccc atgcgttttc tgcttccgct tgatgtcgtc atcatctttc tttcgatgac
     9661 cacttcagtt gcgcttcgaa ctggcattgc cttgtacaat tccatgtttg cagcttcagc
     9721 ggtgcaggcc gtcgtattgg cattggcagt gaagatcatc gcgctgtact tttttggcgc
     9781 atatcaacat ccacgcgaaa actcggtcgt gaagaccctt cttcaagcag gccttgcatt
     9841 aactttcagc accatgaccc ttacggggtt gtatttactg ctcgcccaac tcggcatcgg
     9901 cagggacttc ccacgcagtg ccttcctcat tgaccttggt ttgagcgcct tcctcatcct
     9961 cgcactcagg ctggcgggct actggttcgg aaacagagca atcaatccgc agaactacac
    10021 agacaaaaaa gaaaaaagca tattcaacaa tgctcccctc aaggaattcc aaagcaactg
    10081 gaaaacatgg ttcaccgaag ccattcctta ttatgggatc gtcggtggag cgttattgct
    10141 ttacatgctt ttcaatcgcc ttgttttcgg aacctccagc ccggtcagcg gacaggtcaa
    10201 acgctggtgg ggtacgcttg gcgaaaccgt atatgacagc cctgccccaa attggacggc
    10261 ctttttcggt ctcagttttc aaggcgctta tgatgcctgg cagcctgcat cgggtttatt
    10321 cttgtggctg gtcaaggtca actttccgaa gttcgcagat atccccatcg tgagtgacct
    10381 ctcttacacg tttctcgcgc ttttggttgc cgtggcagtg atcacgcttt tcgtcaacgc
    10441 caaccgcaca cggcgggcgt tttcaaacat ggctctttta cccctcatgg cagggtgtgg
    10501 aattcagatc cttgcgtaca ccaccactgc gtacggcggc gcgaaggaat ggtactgggt
    10561 cagccagatg gttttcgtca ccctgcttgg tagtgctgtg ttggactttt tgattcgccc
    10621 gctgttgaga acgaaggcgc aactcgccat tcaatatgtt tcagctgcgg cctgcctgct
    10681 ccttgcttat cgcttcggcg cacaggtggt caagatcatg ccgtataact tctttccacc
    10741 tgaacgcccc tacatggatg tgctgccata tcttgaagaa aacaccccac caggcagcat
    10801 catcggcatg acgggcggcg gcaatgtcgg ctactttatt cacgaccgaa ccatcgtcaa
    10861 catggacggc ctcatcaaca gctacgaata tttccatgcc ctgcaaaatg gcgaggctcc
    10921 tgcctacctg cgcgaacatg gcatgaccat tgtctttgcc agtccgcgcc tgctggaatt
    10981 gccgccgtat ttcggtcaat tcaatccata cctcgaaagc tacagcgaat atggtggcaa
    11041 gggtttgttg tatcttttgg ataagccaaa atattaaccg atgcaaatat ccataccttt
    11101 ctgttggatg gactttccga atctttaccc agacccaggg tctgattctg ttccaaaggc
    11161 aattttcacc cgatgaagaa gttttccttt cgagcaaccc tgctccagat gctggtgtta
    11221 accctaacag cattcaatct cctgcggatg tggacagccc ttgcctggca ggacatcctg
    11281 accgagttct ccacgcagcc atcccccatt gttttggcag tcagcggcgc ggtctgggcc
    11341 ctgtgcggaa tcgtcctgtt gtggggcatt tggcagaaaa ctgcctgggc cgaaaaactg
    11401 ctccttggcg cttcagcagg ctacagtttg tggtattgga ccgagcgtct cgcctggcaa
    11461 aatccgcacc caaattggtt gttcgctgtt atagtaaatc tggcaatcat tgtttttatc
    11521 ctgttttcaa aaaaatcact gacgagagag gcctatgagc gaaacatcga aaatccaaaa
    11581 attgactgag ctgaaagcac aatcccgcct tggcggcggc caggctcgca ttgacgcaca
    11641 gcacaaaaag ggacgattga ccgcccgcga acgccttgac ctgttactgg acaaaggctc
    11701 cttccgcgaa gtggacccat tcgtcgtcca ccgcacacat gatttcgggt tggacgagca
    11761 aaaattcatg agtgactcgg ttgtaacagg ctggggcacc atcgaaggtc gtctcgtcta
    11821 tgtgtactcg caggatttca ccgtcttcgg cgggagcctt ggcgaagtac atgccgaaaa
    11881 gatctgcaag atcatggata tggccatgaa gaatggcgcg cccatgatcg gcctgaacga
    11941 ttcaggcggc gcgcgcattc aggaaggtgt tgtagccctt ggtggctacg ccgatatctt
    12001 cctgcgcaac acgatggctt cgggagtcat cccgcagata tctgccatca tgggaccgtg
    12061 tgcaggcggc gcagtgtact cgcccgccct gaccgatttt atcttcatgt cgcgcaacac
    12121 ctcctacatg ttcgtcactg ggcctgatgt agtgaaggct gtgacccacg aagaagtgac
    12181 acaggaagaa ctcggcggcg caggtgttca ctctgaaaaa tctggtgtgt gccacgtagc
    12241 cgcagacagt gaagcggata ccttgtacct catccgcaag ttgttgggct atctaccgca
    12301 aaacaacatg gaagatgccc ccttcgtgca aagcgacgac ccactgcgca tggacgaagc
    12361 gctgaactcg atcattcccg atgacgccaa caagccctac gacatcaaag aagtcatccg
    12421 catgatcatg gatagcggac agttttacga gatccacgag aactacgccc agaacgtggt
    12481 ggttggtttt gcccgtttag gtggtcactc tgttggcatc attgccaacc aacccgcagt
    12541 actggctggc gtgctggata ttgacgccag tgaaaaggcc gcccgtttcg tccgcttttg
    12601 cgatgcgttc aacattccca tcatcacttt tgaagatgtc cctggcttcc tgcctggcac
    12661 cttccaggaa catcacggca tcatccgctc tggcgcaaaa ctgttgtatg cctactgcga
    12721 agcgaccgtt ccgaaattga cggtcattac ccgcaaagca tacggcggcg catactgcgt
    12781 gatgtcatcc aagcatattc gcagcgatgt aaacctcgca tggcccacgg ctgagatcgc
    12841 cgtgatgggc cccgacggag cggtgaacat catcttccgc aaggaactcg atgccgcagc
    12901 cgaccccgtg caaaagaaag ccgaactcgt tgcggaatac aaggaaaaat tcgccaatcc
    12961 ctatgtagcc gcgcagcgcg gttacattga cgatgtgatc gagccgaagg aaacccgtcc
    13021 gcgtttgatc aatgcgcttg aaatgttgag caacaagcgc gatgccaacc ccgccaagaa
    13081 acacggcaac attccgctgt aaaggaacag ggagacatca tgttcaaaaa agtccttgtt
    13141 gcaaaccgcg gagagatcgc cgtccgcatc attcgtgcct gccgtgaact cggcctcgaa
    13201 acagtggctg ttttttctga agcagaccgc aacgcattgc acgtgcgcta cgccgacgaa
    13261 gcctatttga tcggccccgc accttcccgc gaatcttatt tgcgcgccga taaaattttc
    13321 gatgtcgcca aaaaatctgg cgcagatgcc attcaccctg gctacggatt cctcgccgaa
    13381 cgcgaagact tcgctgaaaa atgcgccgaa ctgggcatca aattcattgg ccccaaacca
    13441 tcgtccattg cagccatggg agataaaggc caggcccgcg caaccgtcat caaagcgggc
    13501 gtgcccgttg tgcctggcac ggaagatgtc ggcaacatga ccgatgacga cctcctgcgc
    13561 atcgccccca cgatcggctt tccgcttctg atcaaagcga cggctggtgg cggcggaaaa
    13621 ggcatgcgcg aagtccgcag cattgaggaa atgccgaccc tgctccagtc cgcgagacgc
    13681 gaggcagagt cagctttcgg cgacggcaat gtgtatctgg aaaaactcat cgaaggcgcg
    13741 cgccacatcg aattccagat catcgcagat tcatttggca acgtcattca cctcggcgag
    13801 cgcgaatgtt ccattcaacg ccgccaccaa aagcttttgg aagaggcgcc ctcgtcctac
    13861 cttgacgatg acctgcgcac caaaatgggc aacatcgctg tgaaatctgc acaagccgtt
    13921 gactatatca acgcaggcac gatcgaattc cttgtggata aagaccgcaa tttctacttc
    13981 cttgaaatga acacccgcct gcaagtggag catcccatca ctgaaatggt gactggggtg
    14041 gacatcgtgg ccgagcagat ccgcatcgcc cgcggacgac aattaagcta cacccaggac
    14101 cagatcaaat tcaacggtca tgccattgaa tgccgcatca acgccgaaga cccattcaac
    14161 ggattcctgc cttccactgg gcgcatcacc cacagcctga tccccactgg ccctggcgtg
    14221 cgcgtggata cgggtgtgta ccctggcttt gaaatcaccc ccttctacga cccgatgatc
    14281 gcaaaactca ttgtgtgggg cgaaactcgc gcgcaggcca ttttgcgcat gcgccgcgcc
    14341 cttgaagaat atcgcatcgt gggagtacgc acgaatattc ccttccacca aacactgatg
    14401 gattcgcatc gcttcatggc tggccagttc gatacccgtt tcgtggaaga acgcttcagc
    14461 atggacgacg ccgtcgaagc ccgcgcctcc agcgcagaga tcgccgccat tctggccaca
    14521 ctcgttgaac accagcaaag cgagcggtct gcacaggtgg tgcgccgcaa tgaacgcgac
    14581 acaagcaact ggaaatgggt tggccgctgg gaacgcatgc accgctaaag gatgatacga
    14641 gatgaaatat attacaacgg ttgacgacca gcaatttgag atcgaagtgg tggacgagcg
    14701 ccatattcgc attggcggcc gcttgctgca agtggatttt gaatcggtca gcggacagcc
    14761 cgtattctct ctgcttctcg atggacattc ctacgagtcg ttcgtttatc agggcgagga
    14821 agattgggat gtgttactgc gcggcagaca gtatcaggtc aaggtggagg atgagcgcga
    14881 gaaacgtctc agagccgctg cgggtggagg aattgccgaa ggtggagaat tccatctcaa
    14941 agcgccgatg ccaggccttg tggttgccat cctcgtagaa gagggacaag acgtaaagaa
    15001 gggtcaggtc ttgctcatcc tcgaatcgat gaagatgcag aacgagctca aggctccgcg
    15061 cgatggcacg atcggccgca ttcgtgtgaa agcaggcgaa agcgtggaac aaaagcaagc
    15121 catgttgagc gtgcattaaa tgggcgaaca ggaaaccgaa gtaaaatttt ttgtgcgtga
    15181 cctcaaaaag gtcgaattgc gccttctcga attgaaggcg caattgattc aaccacgtgt
    15241 gcatgaaatc aatttccgct atgacctgcc agatggcagt atgcgcacga acggacgggt
    15301 gttgcgcatc aggcaggatg ccgtcgccac catcacctac aaaggcccaa gcgatctggt
    15361 ggatggcgtt ttttcacgcc aagaattgga aacccagatc ggcgatttcg aaacagcaca
    15421 caagatcctc attgcattgg gatacgtaaa aatcctgacc tatgaaaaat tccgcgccgt
    15481 gtatgaaatc gacggctacc acatcatgct cgacgaatta ccctacggcg attttgtgga
    15541 agtggaaggc gcaaacggag cgggcatccg cgcaatgtcc aaacggctgg aattgaacat
    15601 cgaagccgcc gtcggcgcag gatatgcacg catttttgag gattacaaca cccgacaagg
    15661 gcgcgcctca agcgacctga catttgatgc gctacgaggg aaaatcccct ccccggaaga
    15721 gttacgcatc cgcgccgcgg attaagaaaa tagcaacaac ttccgatcac aggacgccaa
    15781 acaaacgcaa agacaaccgt tctttagctg gctggcgtcc tgtttcattt tcacaacatg
    15841 gcgcttgact tcacccgcaa aaagaaatac tatttccaag aggtgaccat gaaactcaaa
    15901 ggcttggatt cgctctccaa acatgtccct gaactgaatt caaccagcgg cagaacgcgg
    15961 atcgttctct acgttgtgtc cctgctggct cttgtcacgg catatttcat cgtaactgac
    16021 aacatcccca cctggagtat tgatagcgag atcattattg cctcgctggg atttttgatc
    16081 ttaagcttgt ttttctcccg caaacctgtt tatcaagaga agtataaaga cctcgcatac
    16141 cgtaatgcct ttgcacatta cgccctgcct gggttatgcc tgatctttgg tgcggtcgct
    16201 catgcaggat acatgaacgg tcccgtgatt cccacaggct ggtggaccac cgtcttcacc
    16261 gtcctcggct ggtattgggt tttggtcggc gcaatacttt ggataagaag catcgccaca
    16321 tttggcgtgg acaatctcaa cctgctctat gtttaccatc ccgaagaaag ccgtatcgta
    16381 gattcaagca tttatgccat cttacgccat ccgatctacg ccagtgccat ccgcgtgtgg
    16441 gttgggctgg ctttgttaaa tggcaacggc aactcggtca ttttttcagt atttgtaccg
    16501 ctggggttga caggctggat tcgccttgtg gaagaaaaag aattgctcga acgctttggc
    16561 aaaccgtatc ttgactaccg caaacatgtc ccagcctttt gggcaaagcc acgggacatt
    16621 gggaagttct tcaagttttt gatcacaggg aattaagtca tgggcatgct caaaaacaaa
    16681 cccgaccaat tacaaaaatt cctattcaag gtccccgtct ggctgcataa aatgggattg
    16741 ggcggctggg aacgcctgat cggcgcccaa tggatgctca tcaccaccat tggaagaaaa
    16801 acggggaaac gccgcgatgt gatggtggac gtaatggact acaacaggaa cacagacacc
    16861 tactatatcg aggccgctta tggcgcgcac gccgattggt acaaaaacat tcaaagcaat
    16921 cccatttttg aggcaagggt tgggcgcagg aagttcaagg cacaggcagg cgcgctttcc
    16981 acggaaggtg ccggggaaat gcttgtacaa ttttatcgag ccaagcccgc ctacacccgt
    17041 tctgtcatgg ccatggcagg gatgaaattc aaagacgaag aagaactccg cctgctcgga
    17101 aaaaacctga ccctactggc ggtaaaacct gaaaaagagt aaacatcatg gaaaagattt
    17161 tgatactggg cgggtatgga tatacaggca aattgcttgc caaatatttg ttagctcaaa
    17221 cgaatgtcca aatcatgatc gctggaagaa gtttggaaaa agcgaaacat ttcaccagcg
    17281 aattgaataa cccgcgcgca accgcgattc aagtggatgc cgccagtttc gacagccttt
    17341 tgcaagcgct tcaaggcatt acattatgtc tggtcgctgc gccgacgaca caccacgccg
    17401 agaaagtggt ccgcgcctgt attcaagcgc gcgtggatta tctggacgtg caatattctt
    17461 caagaaagct tgaggtcttg ttcgctgcca aaaaagagat cgagcaggca ggtttatgtt
    17521 tcgtcaccga agcaggattc caccctggct taccttccgc attgattcgc tatgcctcta
    17581 aaaaattgga tgtactcgaa tctgccttga tcggcggata tctcaacatg aaggaactcc
    17641 cctacaccga agctgtagat gaactcatgg aaggtttcat caattatcaa gcacaggttt
    17701 ataaaaacgg ctcatggacc aagcctgccg catgggattc gcgtcccttt catttcggcg
    17761 acgacatcgg caaacgaacc gcatattcca tgttttttga agaactgcgg ggcatcccaa
    17821 aactatatcc ctcgcttaag gaaactggtt tttatattgc aggatcaaac tggctttcag
    17881 accttgtcat tacaccgatc atttttgtgg gtctcaaact cgcacccaaa cgcgggcttc
    17941 atccactcgg gaaattgatg tggtggagca tgggaaaatc caaaccgccg tatgtggtgg
    18001 cgctcaaagc ggaagcaaaa ggattgttaa acggcagaca ggttgaggtc catgtaagaa
    18061 tcgcacatcc agacggatat gaattcactg cgattccagt cgtcgcgttt ttgaaacaat
    18121 accttgatgg aagtgcccgc cgtgatgggg ttcacatgat gggacatatc gccgagccat
    18181 tgagactatt caaagacatg caggaaatgg gcgttgaatt aaacgaaaac atgaccgact
    18241 gacaatcaca tcttttatgg aaatctatgt tcaatcgacg atgaacaatc gcgcgcccgt
    18301 ttctgtgaag gatcgatgtg ggtcggttcc atcgctgacc tgataactgg tgcctggggt
    18361 gagggtgaat ttcctgccat cggccagttc ggtgtgcaat tctccttcaa ggcacaacaa
    18421 aatatggccc ttttcacacc agtgatcggc atgataccca gcgctatact ccaccatacg
    18481 cactcggatg tttccaaact gttgtgtacg ccaaagcgcc atacccgtta tacctttgtg
    18541 ttccgtgggt gcgatctcag cccaattcgt tgttccaaag ggaatatcct gcatattcat
    18601 ggtgacctct gttcttaagc cagctgccgc ccaatggaag cgccagtcag ttcatcgtgc
    18661 aaacggacaa ggtcgttggt tggcaaagcc gcacccacct gcccaataaa acggcaaacg
    18721 ctgaaatcac cgctccgacg gtcgtactca cttaaccagt tttctgtttg caggtcagta
    18781 tcttcgcgtg gaaaacggat ggtcatggac ttcattccca ttcgtcttgg ttttggggtc
    18841 agccgtgcgc ggaaacattc ctgcttcgaa cacaggatgg tgtaaagcgg gtcacagttg
    18901 aactcggcca tcatggcggt ggtcaccgcg ttccgcgggt caaaatccct gcccagcacg
    18961 atcacccgca ctccttgaaa agtttcatag atgcggaaac caaactggga atattttgaa
    19021 gacgccagtt tctcaaccat ttcatacatt ttcgatttat catctttttt cgagaacaaa
    19081 ccactaagtg acagttttgg tttgtcgata tccaaaatca tcatgctcgc cacattgagc
    19141 accttcgccc cgtatcggtt acgggtgatc accgcctgcg gattaatctc ctcaaggacc
    19201 tgttcgcgga tatctacttc atatccatta aatgcacgcg gatcccccgc aatcttacgc
    19261 ttgactctgt ctaatttggc ttgaaccttc gcccgggcat cttccaccga ctcgttcgag
    19321 cctccataaa aagtgcctgt cttctgttcc ccctgaactt cgaaggtctg gcgttcaacc
    19381 gcccaatatt tgtagattct catcgcttct ccttttgaat tccattcaat gtttctacga
    19441 cacctgtaat tgtacataaa aagaagggca gccaccgatt tggtgactgc cctattattc
    19501 ctatttcatt tcccgaccag catccgcctt aaccatgcga atcgatcagg tcatgggcga
    19561 gttgggcatg gcccaattcc gcttctttta cgctgtgttc catctttgca tacagatctt
    19621 tttcatcaga gaaaacgatc tcaaaggaaa aacggttctt acgcttaatg tccttgaaca
    19681 tgctgtaggc aggctggtcc gaataaacga tgactggctt ttgaaaactt gaaagggttg
    19741 cgtacaactc ctgaggaaca gaaccacccg aaaggtcggc cacgataaat tttgccagcc
    19801 ccgccatggt aatgaccgtt tcggtgtaat cgaggctatt gggcctttca aaatcgaaca
    19861 gcatgggtgt gtaatttctg gtcttgaacc aatccatcag gctgtacaga cgttcgagac
    19921 cgccatcttt gaatttacca aggagcaaaa ctcccttgga gttcaagatg ttgatcatat
    19981 tacgcatgtt cttatgactc gacaggtaat aaacgaactg cgccatttca atgtcatcca
    20041 ccatcatggg gatctttcca ctggcaataa gatcggaata cagctcatac gatttctcaa
    20101 tcaccaatct cgattgcttc attgcagggt cggttttcag atcccatgca gatatgccgt
    20161 acacaacagt ttcccgtatc tccttgagtc ggaaatctgc attgacaagg ttcacgcggt
    20221 tgagattgca attgataaac gtagcgcctt caaaaacggc gtccgtcagg atcgtcttat
    20281 taaatttcgt attgacaaag gttgtattgg aaaaatccgc ccgtgagaaa tccccttctt
    20341 caaaatgaca gccaacaact tccgcattcc gcatgttcaa cccctcggca aacgcgaggt
    20401 ggaccgaggc attggtcaga tgaataccgc tgaaatcaag ctcatttcgc cccttgattt
    20461 gcatgccgcg ctcatcgcgg cactcgtacc aggatggacg cgcaaaatgg ggcgggaact
    20521 tattctcttc ctgccattta ttccacgcgg ctgcaccttt taaaatgact tcttcaggag
    20581 gtagggtcat aaatctctcc aagcggaaat ttgattttaa tcatcataca ccattccata
    20641 ggtgaaatcc acagccccag cacttcaatt aattgatcca tgtgatcaag cgttgcgacg
    20701 ggcaatcata ccgcctgtgc agaaagaatc cgctttaatt cgcgcatatc cctgaaaggc
    20761 attgcaccgg cttcacgcag aacctctaca ggtgtgatcg cagcatgacc gtacacccgc
    20821 attcctgctc gaaccgccgc cgtaacacca ggaacgctat cttcaacaac aatacatcgt
    20881 gaaggcggca catgaaatgc ctgcgccgca gccaggaaca catccggagc gggtttggga
    20941 tgcggcactt ccaatccgct gaagatggca ttgccgaaga actccgtcag gttggcaagt
    21001 ttcaatcgaa gcacgatctc atcacgggcg ccgttggaag cgacacagac tgggacggtt
    21061 aaactctcaa cgagttggcg aatcccatcc acaggcttca attcccgcaa agataattcc
    21121 gaaagacgcg aaaagaattc aggcaaaaaa tttacaggcg gcgtccagtt caattttttc
    21181 gatgtggctt caatacgttt gaaaaccgcc gccccggaaa actcacgcaa ggtcgcgtca
    21241 taatcaaggt ggtggccaag ttcagccagc atctgcacat acactttatt ggcaagcggc
    21301 tcactatcca ccagcacgcc atcacaatcg aagatgacaa gatcaaagga ggttgtattc
    21361 ataggcgcgc gattatacag caaacaatgt caaaagctcc tgcactgacc gtacaacatg
    21421 atcaggatcg ccgctcaaca acctgtccag gctctgatgc ccccacgcaa cagcaacgct
    21481 ttggatgccc gcctccttcg ccgcgcggat atcgctcaac gaatcgccca ccataaaaac
    21541 agactcgcgc atcattcccg ccgaaaactg ctcccgtgca agggaaattt tctgcacctt
    21601 ggaaccaggc aaatccaccc catacactgc gcggaagcaa tctagaatgc cgtgttccgc
    21661 tagaaacgca ttcacgtttt gtgttgaatt cccagtcaca acagcaagga cgttctcctt
    21721 tgccagcttg cgcacaacct ctgccaatcc atcaaaaata gcagggggtg actttttctc
    21781 tgcaaatcga ctcaaacaac gccgcacaaa ttcatccacc aaatgcgcag ggacttccag
    21841 ttgaattcca taagtggcaa aagacatgac ttcaagggag ttcaagtcct cttgagttgc
    21901 gatgtgccga acccctaatt catcgcaaac ctcctgccca aattgaagca aatcgcccaa
    21961 cgtgtccgca aggacaccat caaaatcgaa aaggattaaa gacataatag gaaaaatcag
    22021 cgaagtttct tcgttcgctt ctccatgaat ttccagcctg caatcagacc cgccagggca
    22081 aacaaaataa acccaacctg acccgtagcc tcatatccgc gcacaccgtt gaactcaaaa
    22141 ctggccgcaa aatttccgcc aaaccatgca ccgaccagga gaccaatcac ccccaaaaca
    22201 aatccaaaga ccagagctac aacagattga atcagaaacg ctcgcatcgt tcactcctta
    22261 ttcacacgcc atatcaaatc gaaaccagca actttacgaa aaaagaaaag ataaccccag
    22321 ccgcaaaaga gccaaggtaa atgtgtgtaa cttatttgca agatttttta tcacaaactc
    22381 tcccaccaaa cagtattcag taatttgctg actacacgaa aaacacaaag ggattgacac
    22441 ttactctaca tgatacttga tcccatcatc caattttgga agaaatacga tcagcagaat
    22501 aaccacggca atatagatca aataactcac cagtggaaac gaatttgcca acaagatcgc
    22561 acccaaatat gccccattga cgatgacata attcggcgtg tggctgcgca tatattgttt
    22621 gctgaattca gcaaccgcca aatgcggatg ataatagaca taaagcagca tgatgtatcc
    22681 aaccgcattg caggcgatca gggtaaagcc atagaacacg atcggccccg cctgaaaggg
    22741 ataccgcccc accagagaag ttgggaaagg aataaaactc acacacaaca aaagcaggat
    22801 attcagccaa agaaatccgc ggtctgtctt tttgatcgca tgcatggagt tgtggtggat
    22861 gacccaatac aacccgatga tcacaaaact catgatatac gctaaaatgc gcggaacgac
    22921 agaaaccaat gtctccgaaa attgagcata ttccacttca gggaaacgga tatcgagaat
    22981 caacaaggta atcacgatgg caaacacacc atcgctaaag gcttccaaac gggatttttc
    23041 catagtgtgc tgattatacg ggctgggaca gcgactgtcc attttcggct gcaggcagag
    23101 tttgctgcat tccaatggca cacaatacag atggcagact attgcctgcc atctgttgca
    23161 aggtcatttt atacggcgtc tgttcgtttt agtattcgga ttgtgtagaa gcgccctgcc
    23221 agagccaaaa gggacatata caaaacgggg ccatatagcc agatatcatc cagcgtgatc
    23281 gaacggctca atgacaaaaa tggactgccg ttatcggcaa ccatccacac tgcgcggctc
    23341 gaaagcccga tcaagttgac cgtcacctcc cccattgcct ttgccagtgt cgccaaaagg
    23401 gtcaagtcca ataggccagc aaggaaaggc agggccagaa accctacagc ccaaatcggg
    23461 cggacatgca cctccccaga cttgattccg tgccagcgca aaccgagata caagcttgac
    23521 atgccgagga caagtcccag cagtaaatat gaaggtatga aatacaagcc aaagcccact
    23581 acaagatagt gggtcatcaa ccctgctgcg atgaaataga acaaactgaa tgcaagaaga
    23641 tgtttctgaa cggcatttcc aaggtcgttg agaattgttt tcatggtcat ggcaaactcc
    23701 tttttaagaa cggataacat cctttttaca gatgccgccc acatgggctg attgccattt
    23761 tccgcaattt ccacactttc cgccactgtg gattccctca gggaggcttt cacttcatca
    23821 tctgcggatt tccacagctt gaccgccaat tccatgcgcg agccaattcc cagtttggtg
    23881 tacacattct tcaggtgaaa ttcaaccgtc cgctctgtga tgccaagttg gagggcgatc
    23941 tgcttgttgc ttttgccctg tagaacatgg tcaacaactt caatctcacg gggagatagt
    24001 ttgtttaaat gttcgggaag attgtcagcc atcatttggt ttgttttcat caactttcat
    24061 cctttataat tacccatgca attgtaaatc gtaaatccaa aatcgaaaat caaaaatcgg
    24121 acctcccatg accaacccac tcatcgaatg cattcccaac ttctccgagg cgcgacgacc
    24181 tgagatcatt gaccagatcg ttgccgccat tcaatcggtc agcgaagtga aattgctcga
    24241 ccgctcctcc gacctcgacc acaaccgcac cgtcctcacc tttgcaggct cccccacagg
    24301 cgtggaagaa gccgcctttc gcgccatcaa aaccgcttca gaactaattg atctcgacca
    24361 gcacaccggc gcacatccgc gcatcggcgc aacagatgtc tgtcccttcg tgccattggg
    24421 gggtgccaat atggccgatt gtattgccat tgcccaaagg ttgggtcagc gtgttggaag
    24481 cgagctcagc atccccgtct atttatacga agcggctgcc actcgtccag agcgaaccaa
    24541 tcttgaaaac atccgcaaag gccagtacga aggactcaaa acagaaatcg aaaccgaccc
    24601 aaaccgcgcc cctgattttg gtcctgcaaa actgggcaag gcaggcgcaa ctgtcatcgg
    24661 cgcgcgcaat ccgcttattg ccttcaatgt ttatctcacc acagaagatg tggaaattgc
    24721 aaaaaagatc gcaaaggctg tacgccagtc ctcaggcggc ttgcgctatg tcaaaggcct
    24781 tggcttgctg gtggatggcc gcgcgcaggt ttcgatgaac ctcaccaact tccgcgaaac
    24841 ccccattgcg cgggtcgtag aattcatccg acgcgaagcc caacggtatg gcgttggcat
    24901 ccatcacagt gaactggttg gattaatccc cgaagaagcg cttgttgatg cagcggtctg
    24961 gtacacacaa ctggatcaat ttgataaaga acagattttg gaatcacgac tcttcgacag
    25021 tggacctcag accacaggca gttcatcatc cccaagccac ggtctttcca cgcctcagcc
    25081 tgcttccttc atcgaacaat tagccgcccc gacaccaacc cccggcggcg gttctgcagg
    25141 ggcctacgca ggtgccatgg gcgctggcct cgttgccatg gtcgccgggt tgaccatagg
    25201 caagaaaaaa tatgccgacg tggaagccga aatgcaggcg atccgcgtgg tggccgaaaa
    25261 ccttcgccag gaactcaccc aggctgtaga tgacgacgcc gcctccttcg aagtattgat
    25321 ggccactttc aaactgccaa aagataccga cgaacagaaa gccacacgca atgccgccat
    25381 cgcccaggcc acgctcaacg ccgcgcacat tccattacac gtgtcagaag acgccgtcaa
    25441 agtgatggga ctggcactca aatgcgccaa acatggcaat gtgaatgcca tcagcgattc
    25501 aatgtcagga tttgccatgg cgcgtgcctc aattaccgcg gcgggctgta atgtccgcat
    25561 caacatcaat tcactcgaag atcgtactct cggggagaaa atgctcgagg aactggccga
    25621 gcttgaaaag gaagctgaca agctcgaagc agagatccgc gctgttgcga aagaacgcgg
    25681 aggaatataa accaccaaac aaagacatcc gaagccacaa aggtttcgga tgtctttgtt
    25741 ttaacaatag gtaatttggg gtacagtgaa ttgtgatgaa tgtgacttat aattgcgcca
    25801 ttcaaatgca acttgaggcg gatttcaccc cgcaccagat aaaaaaggaa aacgccatgg
    25861 caggaaatgg attaattggc aaaaaagtag accgatacga gatcctcgag aagatcggcg
    25921 aaggcggaat ggcaaccgtc tataaagcct atgacacgag gctggagcgt gaggtggcga
    25981 tcaaatttac caaacgtgaa gccttcccac ccgatcaact ggagatgatc ctcaaacgct
    26041 ttgaacgtga ggccaaggca ttgggcaggc tttcgcatcc caacatcgtc ggggtcattg
    26101 actacggcga gtatgatggc gcaccttacc tggtaatgac ctatctgcct ggtggcaccc
    26161 tcaagcaaaa acttggcaaa cccatccctt ggcgcgaagc tgttgaaacc atcattccca
    26221 ttgcacgggc actgcattac gttcacagcc acaacatcat gaaccgagat gtcaagccgt
    26281 ccaatatttt gctgaccgaa aacggcgaag ccatgctcac agatttcggc cttgtgaaac
    26341 tattcgaagg tcaggatact tctcaacagc tgacagattc cggtgttggc attggcaccc
    26401 ccgattacat ggcccccgaa caatggacgg gcgaaacgac cgccttatct gacatgtatt
    26461 ccctcggggt tgtgctgtac gaaatgatca caggccgccg cccctatatc tccgacaccc
    26521 cagcaggtat tttgctgcaa caagttaacg agccgctgcc acttccaaag acctatatcc
    26581 cagacctgcc agcaaatatt gaagctgtgc tgctcaaaac gctggcccgc aaccccaagg
    26641 accgctatgc caatatgaat gcctttgcca atgacctgca aagcattctt accggcggac
    26701 aggtgaccgc aaccgcagtt gcaaccgata ttttgcgtga acagatgact ggcaaggtat
    26761 ccaaaaagga aattcaggaa gtcaaaacac agatcgaaca aaagcaggca gaacagaaac
    26821 aagcagaggt gcctcaaaga gaaaggacct ccccaacttc gatctcaaat gcaggacagc
    26881 ctaaatcgtc aaaaccgatg gtatccattg cccttctttt tctgggcgcg attggattgc
    26941 tcgtcctatg cggtggatgt tggctggcct actccttcgg cccgtgggca agagaagccg
    27001 aaacacccac tgcgttggct gtcacctctc cagaggttcc cactgccacc ctggagatag
    27061 aagatacacc aacagcgaaa ccaagcgaca cccccgttcc acaggtcaca ccgaccaaca
    27121 cggctgttcc gatccttctc ccccaacagt ggaatggaac ctacagacag gctggtttta
    27181 attccacacc aatttcgatc gtgatcgaaa aaatgaatgg acaaaccttc accgggaaaa
    27241 tggtctggac tggcggaaca aattacaggg gcgcgatcac agttattgaa ggtgagttcg
    27301 taaccgattt tggcgatgcc acagagcaag ccaagtggaa caaacacctc gattaccaaa
    27361 atggagatcg ggatggcata tggatcaaat ggaccgaaac caaattcgtt tcaggcggcg
    27421 gttataccct tggcgggtgg tattacggac atgtcggcaa agacggaagc atgactggta
    27481 tctacttcct caacgaccag gcttcatctt acgccaacga ttcatggaaa ctcaaactgg
    27541 ttaagtaaga aatttcaaaa acaaaaaaac ttccgaagca caaacttcgg aagttttttt
    27601 gtttcgtttt ttactactcg tccagcgttg aaatatcgcc ttccggtaat ccctgcgctc
    27661 tggccttcaa cacacggcgc atgatctttc ctgaacgtgt cttgggaagt ttatcgagga
    27721 acttcacatc ttcagggcgg gcaatcggac ccatgtgctt ggccacgtgc tgacggagtt
    27781 cttcagccaa ttcaggcgag ggcgaaaaac ctgagcgcaa aagcacaaag gtgtgaatcg
    27841 cctgtccttt tacttcgtgg gataaaccga tcgcagccgc ttctgccaca gcagggtgac
    27901 tgaccaatgc cgattcaact tccgcggttc cgaggcggtg accggaaacc ttgatgacat
    27961 catccacacg accaatgatc cagaagtagc catccttatc gcgtttggca gaatcgcctg
    28021 tggtgtaacg gccaggatac ttcgaccagt attgattgac atagcgttcg tcatcgccat
    28081 agatcgtgcg cagcatggca ggccacggat tcaaaagggt caaataccct tcggtgtcat
    28141 cagggacagg attcccctgt tcatccacaa tctcggcctt ctggccaaag aacgggcggg
    28201 tacctgagcc aggcttgagc ggcaccacag gcgttggagt aatttggaac gcgcctgttt
    28261 ccgtctgcca ccaggtatca atgatcggac acttctcctt gccaatgacg cggtaatacc
    28321 acttccatgc ttcggggttg atcggctcac cgacagaacc gagcaggcga agggaggaaa
    28381 gatcgtgctt gttgggccac gcctctccaa agcgcatgag tccacgaatg gcggttggcg
    28441 cagtgtagaa gatggtgatc ccaaaacgtt ccacaacctg ccaccagcga ttcgggtacg
    28501 ggaaggtggg gccgccttcg aacagcattg aagttgcgcc agcgatcatt gggccgtaca
    28561 caatgtagga atggccggtg atccagcctg ggtcggctgt acaccaccag cgatcttcat
    28621 ctttgacatc aaagacatat ttgatggtgg aataggtgcc gaccatgtaa ccgccatgcg
    28681 tatgcaggat ggcttttggt tttccagtgg aaccagaggt gtaaagaatg aacagcggat
    28741 cttcagcgtc aaggacttca gtagcgcatt tgccattggc gatgggcagg gcacacagat
    28801 cgtgatacca gtgatcgcgg cccgcttcca tcgcgacggg ttggcctgtt cgcttgacaa
    28861 caatgacatt ctccaccgac ggacagcgct tgatggattc gtcaacgatg ttcttgagtt
    28921 caacgatctt tccattttgg taggaaccat cacaggtgat gaccagtttc gactggctat
    28981 cgtcaatacg accctgcagc gcatcaaccg agaaaccgcc aaacaccacc gagtgaatgg
    29041 cgccgatctt ggcacaggcc agcatggcga aaacgatttc gggaacacgc cccatgtaaa
    29101 tggtaacgcg ctcacccttc tgtacgccca tggatttgat gatgtttgcc atgcgggaga
    29161 cttcgcgatt catggcgtaa taggaaaaag tgcgctcctg cttaccgtcc tccgattccc
    29221 agatcaaagc gagttgattc ttacgatggg tcttcaggtg acggtcaacg gcattgtgga
    29281 caatgtttgt ttttgcaccg acaaaccact tgaaaaatgg tttattggaa tcatccagga
    29341 ctttatccca tttctggaac cactccagtt cagatgcctc atctgcccaa aacccctgca
    29401 gatccttgcc agccttttca gcaagcgcgt cccaatcttt caagcgcgcc tgtgcaatca
    29461 catcttctga tggataatag acttcgcctt ccatttcctt ttttgccatg acagcctcct
    29521 gagttaggta agaattgtag cggctatttt aatccgcttt cttgaaattg cgtaatttta
    29581 caccttgcag agttcaacca aagttacacg cccttggaaa tatcatcctt catttgctgc
    29641 caggcctgcc gcgcttcagt tacggagcct tcgtcttcaa tggtggtgat atcgccaggg
    29701 tctttatcga gagtgacagc cttcaaaaca cgccgcatga tcttgccgct gcgtgtcttt
    29761 ggcaacatgc tgacaaaatt caactcacca atgaccgcga tggggcccaa ctcatgacgg
    29821 acggtatcaa tcagttcctt tttgagttca gcagaaggcg ttttaccatt cttgagcaac
    29881 acaaaagcag aaatgacctc gccgcgcaat tcgtcaggat gcccaatcac accagattca
    29941 gccacagcag gatgcttgag gaaggcagtc tcaacttcaa tggtaccgat gcggtgtcct
    30001 gcgatcttga tgatctcatc tgcccgcccc gcaaaccaga cataaccgtc ttcatcaata
    30061 tgagctgaat cgcccgtgta gtaaacgttc gggattttag accagtaatc ctttccatat
    30121 ctttcaggtt caccccacag tgcaggagtg agcccgggga agggtcgctt gatgaccatg
    30181 atcccttttt cgttgggtgc gcatggctca ccctcagggg ttaacacagc cacttcaatc
    30241 ccaggcaagg gaatggttgc ggagcctgcc ttggtgggca gcatgccgat cccatatgga
    30301 tttccaaata ctggcccgcc tgtttccgtc tgccacatat ggtcaatgac agggatcttg
    30361 ttgcccagca ttttattttg cagccaatcc cacgcaggcg cattcagaac ttcgcccgcg
    30421 cagaatattc tttcaaggta atcgtgattg actcgactta acggctcatc gccataacgc
    30481 atgagcaggc gcacagcagt cggtgaggtg aacacaccac tgactgcaaa ttcttcgatg
    30541 gcaattctcc agttcgcttc tgcatgcgga tggtccaacg caccttcaaa agccagagtc
    30601 gtacagcctg taatcaaagg ggcataaacg atatagctat gcccaacgat ccagccaata
    30661 tcagaagtcg cccaccacac atcggtgggt ttcaagccat agacccagcg cgccatgctg
    30721 aagatatgca cctgatagcc gccgtgggta tggatggcaa gtttaggttt ggcggtcgtt
    30781 cccgaagtcg ccagaatgaa agcaggttcg ttggcctcca taggcgtgca atccccattt
    30841 tgcccctcag cgtacttaat aaattcctgc caggttatgt cccgcccatc ctgcatggac
    30901 acatcgtcat ttccgcgctt tagcagaata acgtgttcca catcggtctt cgtgccagcc
    30961 aacgcaccgt caacaatgcc cttaagcggc acatctttcc cttttcggta ggtaatatct
    31021 gtcgtgaaaa caaggcgcga accactggcc tgaattctgt cgcccaatgc attctcgcca
    31081 aaccccgcaa ataccacgga atgaattgct ccaatacgga cggttgccaa catcaacatg
    31141 atcgcctcag ggcaggttgg catgtaaatt gtgagacgat cccctttttt aatgcccatg
    31201 ccgcgcaaag ccgccgccat gcgcttgacc tcatacagca tatgagcata tgtaaataac
    31261 tggcgttcac cgcgctcgtt catgtacacg agagccgtgt gcccgcccca accgcgcttt
    31321 acatgatgat caagggcgtt gtaagcaagg tttgtcagag caccggagaa ccatttgaaa
    31381 ttcgggtaat tccattcaaa aaccttatcc caggtcttga accaatgtaa ttctttggcg
    31441 gctcgctccc aaaatgcttc ggggttttcc ttcgcttcct gtaaccagcg attaacggtg
    31501 gggctgataa agttcatggc atccatctcc tttagcggcg gacagtcacc atttcggcga
    31561 atatcctgaa atggtgactg tctcttccaa aaacgattaa aagcttttac ttattcgctc
    31621 caagcgatgc aaccgaagca tcggaactga tatcaatatg atttgtttct ggcagtttcc
    31681 acaaaccgat cacagcgcaa atccctgcaa cgaccatcgg gtaccacaaa cctgcaaagt
    31741 tattgcccgt ggattggatc aaagacagac caatgatcgg cacaagtcca ccgaacacac
    31801 cattgccgat atgataaggc aacgacatcg aagtatagcg gattttggtg gggaacaact
    31861 caacaaggaa ggctgcaatc ggtccgtaca ccagggtcac atacacaact tgaacgaaaa
    31921 ccaacacgcc cagcatgaat gggctgtatc ccgggttaac caccttatca gccgcgttcc
    31981 caacaaaagg agcgtatttc gccatcgtct gataaatggg cagataagtg agaaccgcca
    32041 ggatcattcc cgccatcatg aacggctttc gcccgtattt atcagaaaga tgaccgacca
    32101 ccacgaagag cggtgtccct gccaacaggg ccgcacccac gatcaaatta gaatccacaa
    32161 ggctcacgcc aaaaatcttt tgtagataga acaatgcata gaactgcccg gtgtaccaaa
    32221 ccacaccctg ccccatggtg gcgccaaaca aagccagaat cacaaagctt aggttgtaag
    32281 gatttccaaa actttcctta agcggattct tggaaacctg cttcttttcc ttgagtttga
    32341 catacaacgg tgattcacgc agggagtaac ggatccaaag cgaaagcccc accaacaaaa
    32401 tggagatcag gaatggaacg cgccagcccg tagccgcaaa cgcctcctca cccatgatcg
    32461 agcgagtggt caagatcaca ccaagtgaca caaacaagcc gagtgtcgcg gtgatctgaa
    32521 tccagctggt catgaaacca cggcgaccgt tcggagcatg ctcagctaca taggtcgcag
    32581 caccgccgta ctcaccaccg agcgccagac cctgcaaaat gcgcaacgca atcaaaataa
    32641 tacctgccgc aggtccaatg gtttcagctg tgggcaacaa acccactgca aacgtacaaa
    32701 gacccatcag ggtcatggtc acaaggaagg tgtacttgcg tccgatcaag tcacccacac
    32761 gcccaaatac aatcgcacca aacggacgta caataaagcc aactgcaaaa gcggccagcc
    32821 aggcaatcaa atcacctaac ggagtgcctg tctgatagaa cttgcttgaa ataacggtcg
    32881 ccagactgcc aaaaatataa aaatcgtacc attcgatcat ggtaccagca gcagacgcaa
    32941 aaataatgcg gggtaaacct tgttccacct gctcaccact ggcaggtgtg tttgcagtca
    33001 cttgagacat aagctggatt ctcctttgga aatgacagtt gaatatatgc cgcgccaaaa
    33061 gcgcagtttt acaaaagagg ggaagattta ggcggtatca gtcatcggcg ctccttttcc
    33121 aatggaaaac ttttcctcgc cccaaatcat tgaaaaaaga agctcgaact gaaccatatg
    33181 ttggggagag gattccatta cgcacctacc ttgcacaaga tgagaatcac catccgccta
    33241 ccaataaaaa ttcgttaatg tttcaggaaa aaacacaaaa aaaccgaact ggatacaccc
    33301 aaaaacaaag acaaatgtca ttatttttta cgacatttgt cacatagact ttacagcgca
    33361 gcagatgaaa accccgcttc aaccttaacc acagcctgct gactcgcgct cacgcgcaaa
    33421 aatgatcgcc cctcaaacct accctctggc acggatcaac aacacaggca cattcgaatt
    33481 atgcaccacc cgttcggcaa tgctgccaag cagccaacgt gcagggccag tccgcccgtg
    33541 tgtggacatt gcaatgacat ccacctgcaa ttcctctgcc accccaagaa tcacctcagc
    33601 caccgacccc acccgcaaaa gagtagaggt tttgaaccca tccgccttca actcagactc
    33661 gatcctggaa atatatgcct tgcttttttc ctcttgctca tccactgcag tttgggcaag
    33721 cccaggatct gaaaaagcaa aatcaagcgc agggtttgcc ccgactgtta gcaaaatcaa
    33781 ctcggcccct tcagaatacg ccaacgactt ggcatgcggc aaaacacctt ctgcaacctt
    33841 tgacccatca aggggaacca aaattttcct atacatggtt tcatctcctt ttgttattgt
    33901 ccaaaacgca accgccttat caggcgattg gaaaatttct cctgtttcat cagcttctga
    33961 accgcggaaa catcgtacaa cacccgtcgg tatacatagg tcatttcttc agtgtcgatc
    34021 atggcatagg ctgcgcgggg atcttcatca cgcggctgcc ccacacttcc ggggttgatc
    34081 cacattcggt cgagagtcag ctttacggga gaattgatgc gcggatattc ctccagcacc
    34141 cgccaatcat cccagggctt ccgaaaaatg acagggaaat gcgtatgccc gttcaatcca
    34201 atgggtgttt cgatgagttc aaaactgctt ttggcaatac ccgtatcggt catatattcc
    34261 caaacaggat cacgcggact gccatggaca agggttatgc cgaaatctgt cataagcggt
    34321 cgatcggtca atgtatttaa ccagtcaagg ttttgggtgg aaagcctgtg acgcgtccaa
    34381 aaaatgatct cttgtgcgtc tttcgaaaaa tcccacactg ccgccctgcc cgccacggcc
    34441 atgtcatgat tccccgcaag gcaaacaggg ttgaattcgc gcagtctgtt gatgcactca
    34501 tttggttcag gcccataccc caccacatcg ccgagacacc acaccgcatc gaaactgcca
    34561 gcatctttta gaacagcttc gaaagcagta aagttggcat gaatatccga tatcaccaaa
    34621 atacgcattg tgaattcccg aaaatattat agcgcatttg aatcacggga agttcccgct
    34681 tttcccgaac cagcgagcgg aatataatta agtcatgaaa aaactgtttg gccgcttatc
    34741 aaaagcgccg caaaaagatg aagcccacgc tcaaacatca tttggcgttg ggacaaagat
    34801 ccaccaacgc taccgtctgg atgcagaaat tgggcgcggc ggaatgggca ccatctatcg
    34861 cgcgcacgac attttgaatg acaggaaagt agcagtaaaa atcatcaacg ccgccactgc
    34921 caatgccctg tcacttgatc aatttaagcg cgagatggaa atttcaacaa agctacaaca
    34981 ccctcacata gtgacaaccc atgaagcagg gacagtggac acagacggca agtcactccc
    35041 cttcctcgtc atggaactgg ttcaaggcac aagcctcgat aaggctggga accttaccta
    35101 cgccagaatt atcgatatcg ccagacagat ttgtgatgcg ctggcatata tccacgatca
    35161 gggattcgtg tatcgcgacc tcaaacccag caatatcatt ctcgaaccac gcggatttca
    35221 aaccttcgtc aagttgctgg attttggtct cgcgcgccca cgcggtgagg cctatcttcc
    35281 aaatgaaagc agcctggctg gaacggtctt ttaccttgcc cccgaattgg tcaacggcga
    35341 gcccgccgac gttggctccg acctgtatgc gtttggcgca gttttatatg aaatgatcac
    35401 aggtcgtgtc cctttctcga atgttgatga acagaccatt cttacgcaac atctcaatga
    35461 acaagtcacc ccgcccagcc aatcgcgggc tgatgtgccg tccgcactcg aatccattgt
    35521 gatgcgcctg ttggcaaaaa cgccgcaaga acgtttcaga tcagctcagg aagtgggttt
    35581 tgcgctggag caaatctcaa tcgcaaaaac agccgccgcc attggaaatt tgccagatgc
    35641 catccccgcc gcgccacaag aacccgttac agagctaatc ggcttgctca caaaaagccg
    35701 tttggttacc ttgttgagca acgacgagac tctagcgctt gcggccgctt cacaaacaaa
    35761 agacatattt tctgatggta tctggctgat tcaattaacg tcgatccatg accccgccag
    35821 ggtaatgcca accgtcatgg aagttctcaa cgtaagtgaa atacccaatc gcccgcctgt
    35881 gatcgccttg atcgagtttc tacgcgagaa acatctgttg ttgcttttat ctcattgcgg
    35941 gcatcttacc ggtgcatgcg tgcagctaat aaaaacaatc ctgcctgcat gccctgaggt
    36001 gcgcattgta gcggtcagca ctcaagcact aaacctgccc gaagagatct gttacaaggg
    36061 tacagcccac tgaaatcgcg tcgttattcc acccaatcca aaatcttgta ccacatccct
    36121 gcaaaaggaa gaaaccatgg gctgcctgtg taaagcccca gcggtgccgc gggaaaggca
    36181 aacttcgcaa aaggatgctc tttgatattg cccttcagca tggcctcagc cgttgtttta
    36241 ccgagatagg tcgccattgc cacaccatgc cctgcatacc caagcgagta gtgcattcca
    36301 tcttcttcgc ccacatgggt catttggtcg aacgcaaaat caagcgtgcc gccccaaacg
    36361 tattccactt tcgcatcttt caactgcgga tacactttta gcatttcatt tcgcaggatc
    36421 tcgccgcttt gggccactgt attttcattt tcaggaaaaa acgccgcccg cccgccgaag
    36481 atcatgcggt tatcccacaa acgaaaataa ttcaggaaat gcttgaaatc aaagatcata
    36541 cgattcttcg ggctaagctc atttgccagt tcattcgaaa gccgttcagt ggcaatgatg
    36601 aatgaaccga tgggaatgat cttgcgttgc aaatttctgg tgacattgcc tgtataacca
    36661 gaagttgcca ccaaaacaga atcaacctca agcgagcctc tttctgtttc gacgataaaa
    36721 cgggtcccgt tccgccgaat cctgttgaca cgcgctcgcg cgcagagaat cgcccccgct
    36781 ttggcagccg ccgctgcaag ccccgccaca tattgagcag gattcaaccc cgcgctgacc
    36841 tcatccacca aggcaccgtg gtacacatct gtaccgatct cggtatgcaa gtccttcggc
    36901 gacaccagat gaactttatg gttgaattct tttgccataa aatccacttc gcctttgagg
    36961 gcttcgtaat gcttgggctt attcgccgcc aacaaatggc ctgtgcgggc aaacccacag
    37021 ttgatgtttt cttcttgaac gatctgctct acaagtgaaa ctgaatcaat cgaagcttga
    37081 aacaaacttc tcgccaggtc tcgtccataa cgcgtgatgg cggtttgcat ggccaccttg
    37141 aggccagtga gggtcatgcc tccattgcgg gaacttgcac cccagccaat ggtttcagcc
    37201 tccaaaacca cagcatggac attgttcttt gcaaaagtgc gcgcggcgct caagcctgta
    37261 tatccaccac cgatgatcgc cacatctgct ttttcgggga tgggtgttag attggaagca
    37321 tcaggcattt ggacggtggt gtgccagtaa atgtgttgtt tcattatgca cctnnnnnnn
    37381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctttcc aattcaattc atcgaaggct
    37501 ttgtgcaggc ggtgaccatc ttccacgaga atgtattgca agttggaaaa ggttttctct
    37561 gccagtattc gcacgggttc cagcggcaca atgtcatctt ctttcccgtg gataatgaca
    37621 gttggaacag aaacaggttc gaggtccaga taagaagcga aatggtcgcg gagaagggct
    37681 ggcgcaagga ggattaattt ccgtacctga gatggatgct cgcaggtgaa gaccgttccc
    37741 attaatccgc caaaagagga accgatgatg gtccaatctt ttttatcgcc aaggatcgga
    37801 gccagttggg ccatgcggtc ctcaaaggag cctgtgaaat cgggggtgat catgcctgga
    37861 aataactgcg cgaactggcg ggctttaccg ctctgactgg tgctttcaag gccgtgaagg
    37921 tagattatct ttgagttatc cattggatgc gctttcgtct tttttcttcc tgcgggtgac
    37981 ggtcttttta tcggtggcag cggattcggg tttgggttcg cgtgtggtaa gttttctgcg
    38041 cggtttcttt accacgggtt cggaggtttc ttctgtgaca aaatctgaag attccgcatc
    38101 ttcaacggga ctgggctttg ataatggctg cgggcgcaga aggtcacgta atgcagattg
    38161 aagcggcggc ggaaatgccg atgaaatgcg gcgcagggtc accacggtca tcgggttttc
    38221 agtattcgca aggatatgct tgagcatgaa ggtcgtttca ttcgaagagg cacggtacag
    38281 aaccacgatc aactctgcga ggtcattctg taacgtggat ggggcttctt caatgatcgg
    38341 ctcaacaagg tcgaagatcg gtggaagatt tgcagtggtg gtatccgcga tcatgggaat
    38401 cagggcttga ataccatttg accacgtacg ggtacgggca ggatgcaaat attcgcggat
    38461 caggttgaaa aattgattgg ggttttcacg ccgcatgcgc gccaggcttg tggaaagcaa
    38521 cgccgaacga acatctggat cacggatttg ttgagtccat gcggtgaggc ggggcaaaag
    38581 gcgttcttct tgcggaggga ttcggcctaa taaaaaagcg gccaacagac gggtttcgag
    38641 tgcgccttca tcccaaagcg tgtcggcaag attgagtgcg tggtcggggt tggctgttgc
    38701 caccgctcgc agttcatttt cgatctgggt aagcactgcg ggcggtgtgc gataggtttt
    38761 gaggttggag ccaggggcaa cgttttcttt tgtccgcagg gtgtaattga cgtaaaaatc
    38821 aaggatctcg cgcagatgct tcataaattc atctggcaga aagaagaaat ctgccagacg
    38881 gttggcttgc ttacgaagac gggcgaggtc aatggcgggc atgggaagaa ggtgtcaggt
    38941 gcgaagtgcc aagtgtcagg cgaatcggct tgtaacctga tgcctgacac ttgaacacgt
    39001 gacactcact aatatgcttt cgcgaaatag acttttcgtt tggagggttt gccgcagacg
    39061 acgcaggcac cttcctcatt gggctggtcg aaggggatgt tgcgggttgt ggctttggtt
    39121 tcttccttgg ccttggcttc acactcacgt gattcacacc aataggcaaa ggcccagcca
    39181 tctttcacaa cttccttgag ttcgtcgaag gtggtcacat tgtggatgtt tgcgtcgcgg
    39241 tattcggtgg cgcgcttgag gagcgaagcc tgaatctcgt ccagcaaccc tttaacggtt
    39301 gagtccagat tcgcttgtga gacgaaggct ttgccttctt tcccggggcg atcgcggcgg
    39361 gcgagggcaa cggtcccctt ttcaacatcc ttggggccga tctcaacacg aacgggaaca
    39421 ccgcgcattt cccaatcatt gaatttgaat ccactgctga cattgtcgcg gtcatcgaat
    39481 ttgacacgga caccggcggc ctttagctcg gcaaaaattc ggtcagcgac ttccagcact
    39541 ttggccttct cttcgtcgct tttgaagatc ggaacgacca ctgcttgaat cggagcaagg
    39601 cgcgggggca ggaccaaccc gttgtcatcg ccgtggacca tgatcattgc accaatgatg
    39661 cgggaagaaa tggcccatga ggtggtctgg gcgtaggtca atttgttgtt tttatcgaga
    39721 tattggatct cgaaggcctt ggcgaaattg tcgccaaaat aatgggaggt gcccgattgc
    39781 agggcacgct tatcccacat cattgcttca atggaggtgg tgatctgtgc gccggcaaaa
    39841 cgctcggtat cgctttttgt gcctttgaaa acagggatgg ctgcttcttc gaggcagaaa
    39901 cgctcataaa tatcgaggat gcgatacatc tcttccttgg cttcggcctc ggaagaatgt
    39961 gcagtgtggc cttcgtgcca ataaaactca gcggtgcgca gaaagagctt ggtgcggagt
    40021 tcccagcgca acaccgaccc ccattgcaca atgagaatcg gcaaatcgcg ccatgatttg
    40081 acccactttg aatacatgtg gccaatgatg gtttcagagg tggggcgaac ggccagtttc
    40141 tcttccaact cctcaccgcc gccatgggtg acaaccgcca attcaggggc aaacccttcc
    40201 acatgatctt tttccttctc aaagaaagac atggggatca gggtcgggaa agccgcgttg
    40261 acatgtcccg tcttcttgaa ttccgcatca agccaggatg taatattctc ccagagcgcc
    40321 caaccataag gctttaccac catgcagcca cgcacagggg cgtagtcggc catgtctgct
    40381 cgaatcacga gctggttgta ccattctgaa aaatcttctg cacgggttgt taacttttct
    40441 tcagccattt tttcctctta tcggtttcag gttgcaagtt gacagatcgc aaataaaaaa
    40501 tgattttggc agatccatca gctttgtaat attgttacgg cagttttgac atcttttgcg
    40561 aagtataaca attcgcgctg atgtttgggc atataacttt caaattcacg gaagaactgg
    40621 tcaaaagtgg actgccaacc gctcccgacc agtattagag gcctgggagg cagcgattcc
    40681 acgatcatca aattccacat cagcgagatc tccgtcagtg tgcctgcacc gcccgagagg
    40741 gccattgccg catcgcatcc ttcgatcaat cccatcaggc gctccatcag ggtttgtctg
    40801 cgccgttctt cttttaccca ttgatttggc tttgaattgc gccattcttc aatatcgata
    40861 cacgtcacac caatgacatg tccgcccgct tcatgcgcgc cgcgagagac agcttccatt
    40921 gtgcccatat agccgccagt taaaacggca tgtccacgct gagcaagaag cgagccaagt
    40981 tcacgggctt cttcatatgc ctgtgaacct tcttttggtt gtgctccgcc aaatacagtg
    41041 atgttcatga gattccttgt ggatgggttg aatgtttaat gttgaaggtt gaaggtttcg
    41101 gaagttgctt tcaactattc ctcaacatcc cattttcttg tcttgttcac ttcgattttc
    41161 tttagcacgg cagcttcaag gtcaatacct gaaacggatg ccaattgcaa aaggtacaag
    41221 gttacatcgg ccaattcgct tcccagctcg ttgcggtcct tgatttcatc gccaaactga
    41281 aaatgctcca gtatttcggc ggcttcgatg gaaagtgaca cagccagatt acgcggcgtc
    41341 tgtggacgct tgctgtcagc ctcataccag ccttttgaac ggaccagatc gtgcatttgc
    41401 cgcgtcagtt ctttgatggt gagctctttg tccatgtagt ttttttccaa tttgcaaata
    41461 aaaacggctc accaagtctg ggagccgttt gggcgcatcg aaccgaacct aaccagacct
    41521 ggaggaatta aaaaaggggc tttgagggga tggtttcggc ttaaacatgc gccaattata
    41581 cacacatatt tataaatgag aagaaacttc cttacatttt ggcagaattt gttatgatac
    41641 aggtgaaaat cactccaaga caaatcctgt gcgcttttga ggcattgagc aggtatttca
    41701 ttcaatccag cattctgtta tattcaggct atcagataac cccatgcccc cgattctgca
    41761 atttctcata cgccgtatat tttctgcatt tatttccctg atcatcatca ccatggtgtt
    41821 gtattcaggc ttcatgctca ctccacctga agcacgggca cgcatctatt tacccccagg
    41881 caaaggcggg gaacgcgctt cggaacaata catcgaaaac atcatcaaag agaaccacct
    41941 tcgagagccc tatctggttc aatatttcta ttgggcaaaa tccctgctgc aggggtcatg
    42001 gggctacagc ccgaccctca atgcagatgt attgccatcc cttttgcacc gtactcccgc
    42061 tacattggaa ctggcactgt actccctcct catgctgatt ccgctcgggc tcgccagcgg
    42121 actggtctca gggtggcggc ctggcagcgt gtttgacagc ggcttccgcg gactcgcctt
    42181 ccttggcacc tcaacgccat cttttattgt ttcgatcatt ctgatctcca tcctttacat
    42241 tcaactagac tggcttgccc caggccgcat tgatgtaaaa accgagctgc aaatgttccg
    42301 ttcgggtttt gtggaataca ccggggcgct gaccctcgac agcctgctca ataaacgctt
    42361 cgatattttc atcatcgcgc taaaacattt gatcatgccc gtcttcaccc tgtcaattta
    42421 ccattgggcc acccttggac gtatcacccg tgccagcatc atgaacgaac gcggcaagga
    42481 atatattgtg gcagcccgtg cccgtggtgt gaaagaatcc aagctgatct ggaaacatgc
    42541 cctgcgagcc attctggcgc cttcgttgac agccatggcc ctatccgctg ccagcattgt
    42601 gaccgggctg ttcgtcaccg aaatcattta cgggttgacg gggatatcgc aaatcattgt
    42661 gatcgcaatg agcagttacc cggatgctcc cgccgcattg ggtttttcag tgtacagtgt
    42721 catcatggtg cttgggctaa tgctcatcct cgactttatt caagccaccc ttgacccgcg
    42781 tgtccgcgag gaagttttga aagcatgaga atcgtccgct atttcttttc acgctggcaa
    42841 aattggctcg gtctgctgat cgtactgatg tttactgtgg tggccatatc cgcaccattg
    42901 ctctccccgc ctgataaaaa cacccaaggt tcgttcaaaa aggtcggtcg ttcctccgat
    42961 agaacccccc acccgccaag cgaaacctcc atcctcggaa ccctgccggg gcaaattgac
    43021 gtgtatcaca ccctggtttg gggggcgcgt gaagccatgc aatttggcct gtccgtggcg
    43081 atcggcgcct tcattttcgg caccctgttt ggttctgtag cgggttactt cggcggattt
    43141 gtaaacaaca tcatgatgag gatcgcagat gcgttcctca cctttccagt ggtggcaggg
    43201 gtggtgctgc tgcaacaact tgtggcagtc acgatcgaat cgctgggtgg aatctactgg
    43261 ctcaacaatg actactttgg tcgggtgatc gattttcaat ttacaccgcc acccttcgcc
    43321 gtttttctga tgaaggtcga tcccatcctc atcagcctga tcattttttc atggatgcct
    43381 gttgcccgca tcgtcaacac aatggttctg accctgaaga acaaggaatt cattcaatct
    43441 tcccgcgcac ttggcgccag ccctgcatgg atcatcttcc gccatctcat tcccaattcg
    43501 gttggtcctg ccgtggtatt ggcagcacgt gatgtgggaa gttccgtcat tcttcaagcc
    43561 acgatcactt ttgtagggct aggcggcgct tcagcctggg gcattctcct ctccattggc
    43621 cgcaattgga tcattggccc gggcgggggc ttgctccgct attggtgggt cttcctgccg
    43681 gttacaattg ccattgtcct ttttggtgtt ggttggaatc tgctgggcga tgggttgttg
    43741 gaagtccttg accccgcagc aaatgcctac ggtggcacgt ctatgattaa acggaagcag
    43801 gccaaaggta agaaagaagc gcaatcggca agtgattcaa aatcaattca tcatgactct
    43861 gattctgtcg cagcacaaac agcgacgcaa ggaatttctg tgaccttaca aaacaatcca
    43921 cccgtgagcc attcacaaga gaagcatccc gttttacaag tggcacgtga agccatcgca
    43981 aaaaaagatt ttgaacatgc catacatgcc tattctcatt tgataaagca tcgcagaaat
    44041 ttgaacgaga tccgccagga catgatcgaa tacgtgcgcg agcatgccaa cgaagctgtc
    44101 atttggactt tgcttggaga tgttctaacc caggaaggga aaatggaata cgcagaaaaa
    44161 gcctacgaac acgccaggaa cttaacccag tgatggatca aaggccgcct gccaaatgaa
    44221 actggacctg gaaaacgaac gcatcctctc caaccgacaa ctgactctgc tgcgagacat
    44281 ttcctccctt tcagcagagt taatgattcc ctgccacctt gtaggcggct cagtgcgtga
    44341 tctgctgctc gaacaaccca tccacgacct cgacattgtc ttcgaagggg atgcgatcag
    44401 ttttgggaat atccttgtca atcgcttcgg cggcaggctg accgcacatc aaaaatttca
    44461 taccgccatc tggcatgtac cagaatctct tgcggaacaa attcaaactc cctttctcga
    44521 cctgatcaca gcccgctctg aaacctacgc acggcccggt ttgcttccaa acgtaacccc
    44581 ctcgaacatg gaagatgacc ttcgccgccg cgatttcacc atcaatgcca tggcaattcg
    44641 tctggatggc gaacacttcg gcgaattgct cgacccactc aatgggcagc aagacctgaa
    44701 gcagggaatc atccgcgttt tacatccccg ttcatttatt gatgacccaa caagaatctt
    44761 tcgcgccatt cgatacgaaa gccgctattc atttcaactt gacccaacca cccgcgaatt
    44821 ggtcaacgct gaagcgcttg atgttttcag gatgttaagt ggcgaaaggc ttcggcacga
    44881 acttgacctg atcttcgaag aagaaaaagc gctgaccatg ctccagagat taagtgaatt
    44941 tgacgcttta agatacattc acccgaagct gccagcattc aaccccactt acgcagatca
    45001 ccttgaaatg gaaacaggtt tggaagtgcc cgccagccgc accaccctgg gctatatgtt
    45061 ttggctgctt gatctttcag aagatgacat cgtgtccatt tcgcggcggt tggaatttac
    45121 atcggaatta actcattcgg tatgggcggc ttcgatatta agaaaaagtc tgcccttcct
    45181 gatcggctcg tcaccaagtg tttgggcatc ggcccttgaa aatctgccgc tgttggcagt
    45241 gtacgcggtg tatctcgaaa caggagaagg ggcgttgttg agttacatct cattctggcg
    45301 tcatgttaaa gcccgcacca ccggcaatga cctgaaagcg ctgggccttc cgcccggtcc
    45361 gcgttacggc gaaattctca cccagctccg cgccgcctgg ctggatggaa agatcacaac
    45421 agaaaaagag gaaaaggagt tgctgaatac attgttatga acctccaacc atatcttgac
    45481 tttgccacct cccttgcata ccgcgcaggc cgaatcacaa tgggttatta caacaccgga
    45541 gtgcgtgcgg atatgaagga aaacaatgag ccggtcacca ttgccgacca cgaagcggaa
    45601 aaattcatcc gcgctgaaat tgaaaaaaat taccccggtc atgccatcat tggagaagaa
    45661 ttcgggcata gccagggaaa cggctcccct tttcgctgga ccatagatcc cattgatggg
    45721 acgaagtcat ttatccgcag cgtgccgtta tacggtgtgc tgatcgggct ggaaatagaa
    45781 ggccgaatca gtgtcggcgc agcgtattat ccgccattgg atgaaatgct ctgtgccgcg
    45841 gacgggttgg gctgctggtg gaacagccgc cgcgcccatg tttcaagcgt ggatgaaatt
    45901 tccaaggcct gtttagtcac ttcagatttt gaacgtcttg aagaaaaaga ccccaccctg
    45961 agagaacgct tcgccagaaa aaaagccctg cttcgaacct ggggtgatgc gtatgggtac
    46021 atgctcgtcg ccaccgggcg ggccgaggta tgtattgatc catttctgga tatttgggat
    46081 tacggaccat atcctgtaat cttgcgagag gctggcgggt attttggtac ctggtctggg
    46141 gaggaaggtc ataccccagg tgatgcatta gcatgcaacg ccgccctgaa acaggaagtg
    46201 atcacgctga tgcaaggctc ttaaaacgct gatttgcttg ctagcaaaca tcatcctaat
    46261 ttataatccg cccatgtcca acaccgagag attgccttac aggttacgcg agcctttcct
    46321 cgcaaccaca gaattggcac tttttcgtgc gttgcaacag atggttgggg cgagatatct
    46381 ggtctgtcct aaagtggccc tcaacgatat tttttatatc gtccgcccaa atgaaaatgt
    46441 acacttcttc aacaagtttt tccgaaaaca tgttgatttt ctactctgtg atccgcaaac
    46501 acttgcccct gcctttggtg tggagatcgt aaggccgatc gcaaaaaatg aaacccgcga
    46561 aagcgacagg ttcattgaag agttattcat agatgcaggt ctgccgctgg ttcatgtccc
    46621 ctccagtgag acctacgatc cctctgatat tgtctctttg tttcaactcg ctgtcaccaa
    46681 agttggcggg actgctgctt tacgtcttga tacctcttct gattcagtac ccctttgtcc
    46741 ggtatgcgga aagatgatgg ttttacgcat caaccgcgat ggtccgcgtt cagggaaaaa
    46801 atactatggc tgtatggata gtccacgttg cgcaggcttg gtgccaatag actaaaacaa
    46861 aatccgctta gagcggattt tgttttttta gaaatgcctt gccagttcag aagcgtactg
    46921 acttccaacc gaagcgcgat gcctctccag ccaatcacta agatgggtac ggcagcgtgg
    46981 cgcctctttg gaaaccagca ggtttgccat caagccatcc acttcctcgg gggttaacat
    47041 cacatctttg acaaacaggc tcataaattg ggcagccagt agtgcaagcc gcggcggaaa
    47101 aggaagcaat aattttttga cgccaatcga cttcccgatc aattccacca tctctttgaa
    47161 tgtaaattcg tcaggaccaa ctgcatccca gatgtaattc tctttcttgt aaactgcatc
    47221 aacggccatc tcagccaggt catctacgta aacgggagag agtttgtatg agccatcacc
    47281 cggcaggcca aaaacaggca gccttcgaag cagccaggca atattgttga taagaatgtc
    47341 ttccttacca aacaagacgg taggcctcag aattgcgtaa ctcatcccag actccatgac
    47401 cgccttctca ttggcagcct tgccccaaaa gtaaggcaga tgagattttg cagacggatt
    47461 ggtgatgcta atatgcacaa tccgcttgac acccgctttt gcggcggctt ccacaaggat
    47521 tttcgtattt tcaacggcac gcggctgggt gttatcacct ttgtcaaaac ggatccagta
    47581 ggtattgacc agcacatcca taccgctcag gttgcggatc aatccttcct ggtcattgaa
    47641 atccaatgga aacgtcttta ccttcccatc aaacggatca ggacgatcgg ggtgatttgt
    47701 cagggtaaac acctcttccc cgcgttcaag caaacgaccg gcaatatact tgcctgagta
    47761 actaaaagca cctgtaactg caattttcat ttcgactcct tattttagaa ccgacaaaat
    47821 agttttaacg ttggtaaacc caaggctttc caatttcaca accgcccgcg tgaggctgtc
    47881 aatggcattg aagaattcct gcaaggcttg aatgcgtttg taggtaaagt caacctctgc
    47941 cttgtcaccg gtcacatcgc ccgcctcaag tttggaaagg gcatccccca ctgaacgaat
    48001 ggcgctatcg aattcgttat tctgacgttc tttcaagatt gaacggatcg acttgtagaa
    48061 atcatcttcg gccatgtaat aatctttgcg atctgccagt ttggaagaac gatgcaccaa
    48121 ccccatccgc gccaaagtgc ggacctcggt gctgaccgct cctttggtca agcctgtcgc
    48181 ctctacaatc tcatccagcg agagcggatt ttgggaaaga tataacacgg caaagatcgc
    48241 tcccattcct ttgggaaacc cccagaaacg gctgatctgg cttagacctt ctgtaaaatc
    48301 ctgtttaagt tgggaaagtt ttatgggcat atctaccttt tattacgttt agtaattact
    48361 aaacgtataa tacgataaat acccgacctt gtcaagaaca aaaagccgtc caaaggacgg
    48421 cttttcaaca cttacaaaaa ttcatatttc gatcaagggg tctcaatgaa cagctgcaac
    48481 atctccaccc cactgacacc ctgccccact tccagatatt tttgaatcgg cacacgcaca
    48541 accaaatgat ccgtctcact cgcttcaaac cagaattcca caaatccata cctgttttga
    48601 aattcctctt tcggaatctg tacaatcatg gccatgacct gagcaatcca atctccgaaa
    48661 aactcgttat ctaacaaatc atctacaggc aggtggataa aaacatccgt ttccatcacg
    48721 ccgaaagttg tacgcccatc tgcataaaca caatcctccc cgaaaaagtt tgcattggcc
    48781 catgctttgt cattcatctt gcggaccgca agatcgatct tctctgtcag ttcgggcgca
    48841 tcatgagtgg cccacatgaa accacagcct tcatatgcca gtgttggggt gagggattcg
    48901 acaggcgctg cgatctcagg ggtaagggta ctgtctgtac tttgaacatt ttcagacgat
    48961 ggcggttgaa ggttattttg cagggtaaaa accccagccg ccacagagac gaccagggcg
    49021 gtggtgatcc atttttgaaa cttagtcatt gagaaaaatt cctcgccgtc tgatatggat
    49081 ggtgatcagc aaaagaattg gtgggtgcag ccccggcggc gaatctgcca cgaagcacgc
    49141 acccaccaaa aggtcaagca ggaaacgcaa ggatcagtgt ctttagtcgg tatgatccca
    49201 gtcgatgatt aatttggata accgccattg ttgttatcgt cgtcatcgtc atcatcatcg
    49261 tcgttattgt tgttatcgtt tccattaccg ttgtcgttgc tgccgttgcc attgtcgttt
    49321 ccatcttcaa cgtcagggta gtccttcggg tcaggaagtg gatttagaaa atctcccagt
    49381 tcgggaattt cagccagcca ttcttcgatc tccgagctat cgatcaacaa gggatcaatc
    49441 ggcggaaaat tgccttcgat ataggtgtac tcaccagcaa ccagctcaac ttcgtcgccg
    49501 ttttcattct taagggcaca atgtccctca agacaagagg ccttgattct tttcgtctcg
    49561 gcatcgtatt ccacacgcat caaactgccg cgcaccgaag caacgccaga gggagtttcc
    49621 acttcaaggg agcccccgtt caaaagaata taaacctttc caaacagcaa ttcaattacg
    49681 gtctttggct ggccattctc ttggacgatc tccgggacgg taaaggaaga gtttggaccc
    49741 acgcgcacga tcgtgccttc aggcaacagg tcgaggcggg cgcggccatc cgtccctgtt
    49801 tccacaccac cggttggaag aatacttgtt cccacagaag cgggcgccag atcactggtg
    49861 gaaagcgtcc gtgccaaaac ttcattttga acttcggaaa tctttgcctc acgcgctaca
    49921 ggagcctcgg ttgcagtgga caggggttcg gggttggcgg gtatatccac cgcaggccca
    49981 acctgggatg gttcttctgt tgcaaggggc gcttccgtcg gcgatacggg aggcgtacat
    50041 gcgctcagaa gtagcatcaa aagcaaaatg gtaatggtta gttttttcat aagatatcct
    50101 ttctccaatt tctacctgaa tttcattata tactatttca aaatgtcggc tgccgctgcc
    50161 gccataagcg ccgagccggt gatcaacgcc tgttcgtcga aatcaaattt ggggtgatgg
    50221 tgaccgtaat caagatgccg ctcatgattg tttgaaccga taaagaaata acacccatcc
    50281 actttcgcct gcatgtaagc catgtcttcg gcgcccatgg tcaggtaagg tgaaaggtca
    50341 tgatgcaggt ttggaagcag acgttgtgca gtggcctgca cgctggccgc cactctttcg
    50401 gcattgatca gcgccggggt aagctgcttc acattcaact ccacctgaca ccccattgca
    50461 gaggcaaccc cgcgcacaac ttcatcgaaa cgctgtagca ccttcgtccg cacttgtggg
    50521 tcaaaggtac ggatcgtgcc ttgcatcatc acctcctgcg gaatgacatt gaaggcagtg
    50581 cctccgttga tgaccgtaaa gcttatgacc gccgtctcca acggaccaac attgcgcgaa
    50641 acaatggact gcgaagccga aacgatctgc gctgcacata ccactgggtc tactgccaga
    50701 tcgggaatcg ctccatgacc gcctttgcca atgaccttga tcttaaattc ctcagcacct
    50761 gccatcaccg ggcctttggc cacatttacc cagccgagcg gcttttcatt ccacagatgc
    50821 aatgagagag tcatatccac cttggggcca tccaacacgc catcacgcat catcatttct
    50881 gcgccgccaa cttcttcgcc gttattccct tcttccgagg gctgaaaaca aaacttgacc
    50941 gtaccagcca gcttgtcttt gtgctgattc agcagttttg ccacagtcaa cccaatggca
    51001 gtatgaccgt catgtccgca ggcatgcatc acacccgcat tttgcgaggc atattctgca
    51061 cctgtttctt cgtgaattgg caaggcatcc atatcaaaac gcagaagcag ggtaggccca
    51121 ggctttgcac cttcaagcaa gccaaccaca cccgtcttgc ctacaccttt tgtcacttca
    51181 aggccaagcg cttccaattc ctttgccacg attccactgg ttcgaatttc cctgaaaccc
    51241 aattcagggt gaaggtgaaa atcacggcgc aaggtttgag tgtatgaaaa taacgatctg
    51301 gcttcattca gaaaatcggt cattgttttc tcctgattat tttccatggc ctggaaatac
    51361 agttttgatc tggtgttgat aaattctatc ccagcttgcc cgtgaaatgg tatcactctc
    51421 tggtaataca aaaatggggg acaaatcacc cgtaaatacg aaaccttcat caaggaaaag
    51481 tgacacacta tcatcactat gccccggagt gtggatgatc tccccggaaa gccccagctt
    51541 caaaagaaat tcacggctct cttcgacctt caacctgata ctgtcatccg tcgtaattgg
    51601 ccggtaggga tggggtttgg tcttaaaaaa ttccgtcatc tgaaaaacga aatccacctg
    51661 cacatccaat agaatgtgtt tgattccaat cgcttttaat tcctgggtca tgccagcatg
    51721 gtcggggtgg aaatgcgtaa caagaagata tttgatctcc tgaagtctga tcccttttcg
    51781 cttcaattcc gctgaaaatt gtggaagtgt ccccggccaa ccacagtcaa cgagcaattt
    51841 cccgcctaaa atgtcgatga gataataatt tgtcgattgg taaccaacgt tgacaatgtt
    51901 catttttgga agatcaaagg gttgttagac cctttgatcc ttgattatcc gtactttaca
    51961 actttgaatt tctcttcata gcgcggattt tcctccgtgc tgtcttcagt gcggcgggtt
    52021 ttgaaacgtt cgatcaaagc cttgggatcc tgcacttgtg tcttgtgttc aagaatggcc
    52081 tgcattttga tatcccaggt atcggtcaca tctacactgg tgttgggttg attggtaagt
    52141 gaacaccaga cctctttcgg catatgcggc tcgaagcctt gcgcaagcag gtctgggaag
    52201 taaacaagat tacccgcagc agggaagact gcatccaaaa ccacctgacc acaagcgcgg
    52261 tggtcgggat gattaatacc gtaggtggca aacaaagtct gcgggtcgca ggtgacgagg
    52321 atatcgggct tgtggcggcg aatctcgcgg acaatgtcac ggcgtagatt caggtctggg
    52381 atgaggtacc cgtccgggag gtccataaaa tgaaccgagt ttgcgccgat gatctttgct
    52441 gcgttattct gttccccatg cctcaatccg cataattttt cgggcgtcat ttctgggttt
    52501 gtggtgggat taaacccctt atcaccacaa gtcagaagaa ggtaggtgat cttgtgccca
    52561 tcgcgggccc atcgggccag ggtggccccg caaaaaaatt cggggtcatc ggggtgggca
    52621 agaatcacaa gaatattttg gggggaagtc cagttttcag gttgaagggt catgggaaat
    52681 ttgaaaataa agcacgaatc tttttggggc aattcgtgct ttttaaatta gtttttgtta
    52741 ttgttgttgc gaggagcttt tccgcaggta cagccgccgc cttcgtggcg gtcacagggg
    52801 gcctgcgatt ttctgcgcaa ggcttcaagt tcatcccacg gttcgatctc ttcacgagca
    52861 aaggtcattt gttgggggcg gtcaacgccg ctttcgatga gcaccactac tttatcgctc
    52921 atgggaacaa catcaatcac tttgccttca cccttgggtg taaccacgcg cttattgcgc
    52981 ttcggcaggg ttttgcgggc ttccacatat tgctcgtact catagataag gcagcagcgc
    53041 aaacgcccgc acatgccggt gatttcattg ggcgtaaggg aaattccctg ttcctttgcc
    53101 attttgatgg aaatgggcga aaaatcagta aggaacttcg aacagcagcg cgtttcgatg
    53161 ccgcaagctc ccatgccgcc caaaaacttc gcaacatccc gcggaccgat ctgacgcatt
    53221 tcgatctgcg cattgggata aatttgttgc atgtcctttt tgagggactt caggtccact
    53281 ttatcttcac tttcagtgct aaaaaggaaa gtgaggcggg aaccatcata gttgtactcg
    53341 gccgtgacga tcttgacatc ccgcagatgc aattcagaag cacgcgcacg gcagttgatc
    53401 atggcttcgg tttgcctgga ttgccaggat tgctgaagca gtaaatcgcg cggcgtggcg
    53461 cggcgttcca cagccttcca accaccttca ggctggggag gggtttctgt caaaacttgc
    53521 accacctcgc cgagatgctt tccgcgggtg gtatcgacaa tcacatgttc gccctgcttc
    53581 acatcaggaa gagtggaaga atcaaagtgg tagattttgc caatttttgt aaatcggaca
    53641 cctataatat tcgtttgcat ggggacaatt ataccgtagg gaatggctag ccaaagccca
    53701 ttttcgggtt tccacattgc aattcttgat gctttcttga tgcatttccc cgccttcttg
    53761 ccgccaaatt gaccaaatca ctcataaact tgctgtattc aaatgcgagg agaaaccatg
    53821 agcgatcttt tatacctggg ttttaccgtc cttttctttg ccatcaccta tggccttgtc
    53881 acggtctgcg aaaggctcgg ggaggaaccg aaatgaatac actatacgca atcgttggca
    53941 ttatcacatt gctcctgttc ggatatctgg tactggcact gatcaaaccg gagtggtttg
    54001 gatgaacaca aacaacctgc ttcaactcat gtttttcatg ggaactttac tactgctcgt
    54061 aaaaccgctt ggctcataca tggcaagggt ctatcaaaac gagcgcatct ttctggaccg
    54121 tgtactgggt cctgttgaaa gattaattta tcgcgcctca ggcatccgcc ccaccgaaga
    54181 catggactgg aaatcatatg ccgcggctct gcttgtcttc aatatgttgg gattattcgt
    54241 cgtttatgcc cagcagagat tgcaactcgc actccccttc aacccgcagg gattctctgc
    54301 tgtaacgcca gattccgcct ggaatacagc cgtaagtttc gcatccaaca ccaattggca
    54361 gagctacggc ggggagacga ccatgagtca cttcacccaa atgcttagcc tgacggtgca
    54421 gaattttgtc tctgcggcaa caggtatggc cgttttggtg gcgttaatcc gcggaattgc
    54481 ccgccactca gggaaaggga tcgggaattt ttgggtcgac ctcacccgtt ccactttata
    54541 catccttctg ccgctttcgt tcattcttgc catgttgctg gtctcacaag gtgtagtgca
    54601 gaccttcaac ggatatagaa ccgtctcgcc cctgcaatct gcggagcagg ttattgctgt
    54661 aggaccagcc gcatcacaga tcgcgatcaa acaactgggc acaaacggag gcggattttt
    54721 caacgccaat tcagcacatc cgtttgaaaa cccaacccct ttttccaatt ttctggaaat
    54781 gctttccatc ctgctgatcc ctgcggcctt gtgctacacc ttcggccaaa tggtgggcga
    54841 cctccgtcaa ggctgggcat tgctgacagt tatgacgatc atatttctgg ttctcgtcgg
    54901 cataaccatt tggtctgaac tcggcggaaa ccctgcactc ccatctttag gcatcgatca
    54961 gattcaatcg gccagcaacc caggcggcaa catggaaggc aaggaggtcc gctttgggat
    55021 cgttaactct gcgctttggg ccactgccac cacggctgcc tccaatggat cggtcaacgc
    55081 gatgcacgac tccttcatgc cgctcagcgg gcttgtgccc atgtggctga tgcaattggg
    55141 cgagattatt tacggaggcg ttggttctgg cttatacggc atgctggctt tcgtcattgt
    55201 agcggtcttt atttcaggtc tgatggtggg acgcacccct gaataccttg gcaaaaagat
    55261 agaggcctat gaaatgaaaa tggcgtccct catcattctc attccagtaa cagtggtttt
    55321 gacgggtaca gcgctggctg tttcaatccc tgacgggagg gctgccattt acaacccaac
    55381 ggcgcatgga ttcagcgaag tcttatacgc gttttcatct gcagggaaca ataacggctc
    55441 tgcttttgca gggctgagtg cgaacagtcc tttttataac ctttcattgg ggattgccat
    55501 gttgatctct cgttattggc tggctgttcc agtattagcc atcgcagggt cacttgtccg
    55561 caaaaagaaa attcccgcca gcgcagggac attgcccacg catactcccc tgttcatggc
    55621 ctggctgatc ggcgtcatca cgatcgttgg tgcattgagc ttcattcccg ccctggcgct
    55681 gggccccatc gttgaatatc tgacaatcat cggtggttga tattgaaatt caaggaacac
    55741 aaaatgagca ttcaaacccg aaagcacaat tctttccgcc gcgaaattta tcagcgtgca
    55801 gtaacagagg catttataaa actcaatcca cggttaatga ttcgcaaccc cgttatgttc
    55861 gttgtggaag cgggcagtgc attgacaact atattatggg tgcaagcctt gcttggaaag
    55921 ggagaagcgt catctggttt tatcggtacg atcgccatct ggctttggtt cactgtttta
    55981 tttgcaaatt tttctgaagc agttgcagaa gggcggggca aggcacaggc tgagtctctg
    56041 cgaaaagcaa aacaggatac tcccgccaaa aaactcaaaa aaacacagct tgatggtgca
    56101 accagcgagc acgaggtaat ttcatccact ttgctgagaa aaaacgactt atatcttgtt
    56161 gtcactggcg atatcctgcc tgcagatgga gagatcgaag cgggaattgc atcagtggat
    56221 gaaagtgccg ttacgggtga atcagctccc gttgtgcgcg aagcgggcgg tgaccgttcc
    56281 gctgttacag gcggcacacg tctcctttca gactggctca tcgtccgcgt cagcgccgac
    56341 ccggggcagg gattcctcga ccgtatgatc aacatggtgg agggcgcaaa acgacagaag
    56401 actccaaacg agattgcgct caatatttta ctggctggat tcactcttat tttcctggtg
    56461 gtctgcgcca ccttgctgcc ttattcgatt tacagcgtag aggcggcagg caaaggaaca
    56521 gccatcacca ttacggtgtt gatcgcattg cttgtgtgct taatccctac aaccatcggc
    56581 ggtttgcttt ctgccattgg catcgctggc atggatcgca tgatccaacg caacgtcatc
    56641 gcaatgtcgg gcagagctgt ggaagcggca ggcgatgttg atgtattgct gcttgataaa
    56701 acaggaacga tcacgctcgg caatcggcag gcagttgaat tcatccctgc caaaggtgta
    56761 acggcaagcg aactcgcaga agctgcccaa ctcgcatctc tggcagatga aactccagaa
    56821 ggccgttcga tcgtggttct ggcaaaggaa aagtttggcc tgcgcggacg cacgatgaac
    56881 gcgcccgcag gcatgcactt tgtcccattc actgctcaaa cccgcatgag cggcgttgat
    56941 tttgacggca tttccatccg caagggtgcg ccagacacaa tgctttcaat ggcagatttc
    57001 aacgggcacc catttcctgc cgacctgaga tcgtcagtcg attcgattgc gcgaatgggc
    57061 ggcacccctt tggtggtgac gcgcaacaca aaggcgctgg gcgtcatcta cttgaaggat
    57121 attgtcaaag gcggaatcaa ggagcgcttt gcacagcttc gcaaaatggg cattaaaaca
    57181 gtgatgatca cgggagataa tcccctcacc gcagcggcca ttgcagccga agcaggcgtg
    57241 gacgattttc tggcacaagc cacccccgaa gccaaactca aactcatccg tgaatatcaa
    57301 tcaggcgggc ggctggttgc aatgacaggc gatggaacca atgatgcgcc tgcattggca
    57361 caggccgatg tggcagttgc catgaacaca ggcacacaac ctgcacggga agccgccaac
    57421 atggttgacc tcgacagcaa ccccacaaaa ctgatcgaga tcgttgagat cggcaaacaa
    57481 ctcttaatga cccgcggcgc attgaccacc ttcagcatcg caaacgatgt gagcaaatat
    57541 tttgcgatca ttccagcagc cttcgcctcc acctaccccg tacttggcgc cttgaacttt
    57601 atgaagctcg ccaccccgca aagcgccatc ctttcggcag tgatcttcaa cgccctcatc
    57661 atcattgcac tgataccaat tgccctgcgt ggggttccct accgcgccaa gccagccgac
    57721 cagttgctgc gtgacaacct gctggtctat gggctcggtg gtttgatcgc accattcatc
    57781 ttcatcaaac tgatcgacct tatcctggtc tggatcggtg ttgcgtgagg attccatgaa
    57841 acgaaaccat caccttctgc catactttat ctacacgtcg gcaattcttg ggtttaccgc
    57901 cgccatcgcc ccagacaaac tcaacattcc aaccaacttc cgtccctggg tattccttgc
    57961 ttcgatcgcc tgggcggttg cggttctttc aggagtattc acagcatgaa aacacatctg
    58021 cgccccgcat ttacgatcct gactctgctc actgtgctca caggtttggt ctacccattt
    58081 ctagtcactg ggctggcaca agtctttttt ccgcagcaag ccaatggaag cctcatatcg
    58141 gtcaatggca ctgtgtatgg ctcggaattg atcggccaat cattcgacga tccaaaatat
    58201 ttttgggcac gaccatctgc cgcagggtac aacgccgcgg cttcttcagg ctcaaattac
    58261 ggacccacaa acccagcctt gatggatgca atccaagcac ggatcgccgc actgcaaacc
    58321 gccgaccctg aaaacacgct tccgattcct gttgacctgg tcactgcttc ggggagtgga
    58381 ctcgacccgc atatcagcgt cgccgcggct ttgtatcagg ttcatcgtgt ggcagcagcc
    58441 cgcgggttga gcgaagcgga agtccgatca ctcgtggagc agaacacaga aggccgtcaa
    58501 tttggctttc tgggtgaacc gcgggtaaat gtattaatgc taaatctcgc actggatggg
    58561 atacaataat tttgatgcaa cagcgccctg atcctgatga attattgaaa cacattcaac
    58621 ctgaagaaca tggccgcggc aaactcaaga tgttcctcgg ttacgctgca ggcgtgggaa
    58681 agacgtacgc catgctcgaa gccgcacacc aacgcaaagc acaaggggtg gatgtggtca
    58741 tcggttatgt tgaaacgcat aaacgggcag ataccgatga acttgttgaa gggctggaag
    58801 tgctgccccg aaaacaggtc aactatcaca atgtgaccct gcctgaaatg gatgtggaca
    58861 ggatactggc ccgcaaaccc gccttggtgc ttgtggatga attcgcccac accaatgcgc
    58921 caggctcacg gcatcccaaa cgctttcagg atgtggaaga gatcttgaac gcagggattg
    58981 acgtttacac cacgttgaat attcaacatc tcgaaagcct gaacgatatc gttgcacagg
    59041 ttacaggtgt gatcgtacgc gaaaccgttc cagaccgtgt cattgacgaa gcatcggaga
    59101 tcgaggtcat tgacctgccg ccagacgaat tactgaccag attgaaagaa ggcaaagttt
    59161 acatccccga acaggccagc cgtgcaattg acaagttctt ccgcaaaggg aatcttaccg
    59221 ctttacgtga aatgtctttg cgccgcgccg ctgaacgcgt ggatgaccaa atgcgctcgt
    59281 acatgcagac acgtgccatt tcgggtccgt gggcggcggg agagcggctt ttggtatgcg
    59341 tcagccccag cccattgagc gaacgcttga tccgctcagc ccgccgcctt gccgatgaac
    59401 tgaacgcaga atggatcaca gtgtatgtgg aaaccccgca atttgcggcc atgtcacagg
    59461 aaaagcggga tcgcgtggcg aagatcctgc aattggcaga agaccttggc gcacgctcct
    59521 acacactttc atcgaacggc tccatcgaat ccactgcaca ggcggtgatc gacttcgccc
    59581 ataaaaacaa cattacaaag atcattgctg gcaaacccct gcggccacgc tggcaggaat
    59641 ttttacgcgg ttcgcttgta gacaaattga ttcacaaaag cggcgacatt gatatttacg
    59701 tcgtcacaag catggatgtc cccgcattcc ccgctgacga aaatccgctg aaattgcaca
    59761 gccctcttta taaatatttt ttgagcatgc ttctggtcgg ttttgccacg atcatcggat
    59821 ttgtgatcgg cggcagaatc gagccaacca acctggtgat ggtctatcta ttggctgtgg
    59881 ttgtggcctc tgtgtatctc gggcgcggcc ccgccgtatt agcctccctg ctgggtgtgc
    59941 tggcatttga ttttctattc gtcaccccat atttcacctt tgcggtttca gacacgcaat
    60001 acatcatcac cttcatcgcc ctgtttttgg tggggatggt catcagtcag ttggcagcgc
    60061 gggccagtga acaggccgag gcggcacgtc aacgcgaggc ggaaactgcc gagctatacg
    60121 acctgagccg tgacctggcg atttctgctg aactcgaatc catacttgct gttttacaaa
    60181 aacatgtcga aaagatattc agccgcaccg tggctgtttt gctgcccaca gaaaaccgcc
    60241 tgacaagcta cattctaagt acagggcttg aattaagcga tgaagaagcg gccgtggcag
    60301 attgggtcta tcgtcatgga gaggctgcag ggcgaaacac caataccctg ccagccgcgc
    60361 aactgcgcta cctgccgcta aaaacagcac gcggcgtggt tggtgtactt ggagttggca
    60421 agccaaacac catcgaacgc gatctcacac ctgcgcaacg ccgcttaatg gaagcctttg
    60481 ccaaccaatg cgcacaagcc attgaacgtg tccgccttgc ggaagaggcc cgccagatca
    60541 aactgttgca atcggctgag aaactgcaaa acgcattgct caactccatt tcacatgacc
    60601 tgcggacacc gctcgtttcg atcacaggcg cactgaccac actggaagaa caagccagcg
    60661 aattggaatc tcccgcacat cgcagtttgg ttgaaaccgc acgtgaagaa gctgaccgat
    60721 taaatcgcct tgtgggaaac ttgttggata tgacccgcct cgaagctggt gcattgaaag
    60781 taaagtgcga gcccagcgac attcaggagt taattggatc cgccatcagc cagatggaaa
    60841 gccgtttgga cagacgcagg atcgaggtcc agataagcga cgcgctgcct ttggtcccgc
    60901 ttgattttgt gttgatcgtc catgtgctca acaatctgat cgacaacgct ttgaaatact
    60961 ctccagaagg atcaccgctt gagatcaatg ccgatcttgt gaaagatgaa atccaaattt
    61021 cagtaacaga caatggcatt ggaattcccc atgaagacct tgaacgggta tttgacaagt
    61081 tctaccgcgt gcaacggcca gaaaatgtca caggcacagg gcttgggctc gccatctgca
    61141 aaggcatcat tgaggcgcat ggcgggcgca tctgggccaa aaacaacgcc gttggcggtt
    61201 ctgtatttac gattgccctg ccaatggagg aggcaaaatg acgcgcattc tggtcgtgga
    61261 tgacgaacgt ccgatccgca ggtttttgaa agcatcgctc gggaatgaat acgaagttgt
    61321 tgaagccgtg gacggagaag atgccattcg cgtcactgcg accgaaaagc ctgatcttgt
    61381 tattctcgat cttggtctgc ccgatatgga tggcgtggaa gtaacccgcc gcctgcgcga
    61441 atggacacag atcccgatca tcattgtttc cgtacgcgaa caggaaactg acaagatctc
    61501 cgcactggat gcaggcgcag acgattattt gaccaaacca ttcggcgcag gcgaactaat
    61561 ggcacgcatc cgtgttgcca tgcggcatgc ttcgtctcct gaaacccagg cagtattcga
    61621 gagtggagac ctgctggtaa atctttcaga ccgcattgta aaagtggctg gcgaagaaat
    61681 atcactgacc cccaacgagt acgatatcct gcgcgcgctt gttcaacacg ctggcagggt
    61741 catgacacat catcaattgt tgcgcaccgt ttggggaaat gcctacgaaa acgaggccca
    61801 tttattacgt gtgaacatga gcaacctgag aagaaagatc gaagctgacc ctgcacggcc
    61861 cagacacatc atcaccgagc ctggtgtggg atatcgactg aagatcgcat gattcaatcg
    61921 acctttacag aggtttaaga gagtgtctga aaaccctacc acgaagcctc gaagacacaa
    61981 aagcacgaag ttttttactc tttgagtctc cctggcaccg cacgccagtg ttgtggtcaa
    62041 aaacagactt ttcagacatt ttctaacaaa taacaaagtg cctcgtcttg gaattgaaat
    62101 tccaagacga ggcactttta cgttcgtata aaaccgtcac cttcgatttg caccccccaa
    62161 aaggtgacgt ctggtcacat atcagttcat tccacgatat tgatcggcaa tgcgttgttc
    62221 gcgctcatgg cagccacact ggctttggca gcaatctttt gagcaatttc ctgaatggat
    62281 ttcgcgacag ccgattcggg atgggcggca acgatgggct tgcctgtatc gccgccaatg
    62341 cggacattct gatcgatcgg tatttttcca aggaaggtgg tgtctgtggc tttggccaac
    62401 tcttcaccgc cgcctgaacc aaacaggtcc atacgggtgc catcgggtaa gtcaagataa
    62461 gacatgttct caaccacgcc caaaatggga acttccaact gcttgaacat attcaagcca
    62521 cggctggcat cttcaagaga aacgagttgt ggaagggtca caaccacaga gccgctaagc
    62581 ggcaaagcct gcgccagaga aagggcggca tcacccgtgc ccgggggaag gtcaatgatg
    62641 agatagtcaa gttcgcccca cacaacatcg ccaagaaatt gacggatggc ggagttgagc
    62701 atgggtccac gccagatcag cggctgacca ggtttaacca ataaacccat tgagatcatt
    62761 ttgattccgt acgcctcggc agggatcaat ttctgtccag agggcggcgg aagtttatcc
    62821 acacccagca tggtgggcgt gttcggaccg tagatatcgg catccatcaa accaacacgg
    62881 gcaccgcttt gagccaatgc cactgcaata ttcaccgaaa cagtagattt acccacaccg
    62941 cctttgccag aaccaatggc aatggcattg cggatcggca tggaaaccaa cccgcgcata
    63001 cggccgtcgt ttggcacgtc agaatccatt ttgaggtcaa tggtcttgac gccatccacg
    63061 gccatcacag cttcgcgcac ttccttttca attcgtccgc gcaaagggca ggcgggagtg
    63121 gtgagcataa cagtaaatga gaccttgtcg cctgatatct ccagattgcg gatcatattc
    63181 agggtgacaa ggtcctgatg tagttcaggt tcctgcactt tgcctaaggc agcgagaaca
    63241 gattcttttg agagtgtcat ttcatttcct atgattgata aattgcgtac aagtaaacaa
    63301 gcagacaggt aaacagtatt cgtgtttacc tgtctgcttc aaatttaggc tgatgcggct
    63361 tcagccttgg cggccttggc ggcttccttg gctttgcggg cttcctcttc gcgggtgtaa
    63421 ttctcggggc ggatctccgc gtaatatttg gcgggcttca gcagcttttc gaggttgtac
    63481 acattgcggt cataggaggc gatatcaaaa tcgtggtcca tcttgatggc atcgaaggga
    63541 caatattcgg cgcagaaacc gcagttcatg cagatgtcca tatcgatgta gaaattcgtc
    63601 ggaacaggaa caggcctgcc cgtggcggga tcgttcgtcc tcacgatcca aatacattgc
    63661 ggcggacaca ccttggagca aattccgcac gaggtacagc gcacttcctt ctttccatct
    63721 gcgccttcat cgtacacaag gaagggcacg tagcgaaact cttcgggctg gatcaactgc
    63781 tcttcagggt attgaaccgt aaagatgcct ttggtgtctt tgcttgaacg atgcgccacg
    63841 ccttcaacag tgttgtagcg tttcttgccg cgcagcatcc aggaaatatc ttccgtgtat
    63901 gtatcccaga atcgcttcca ggtgacgctt aatcctttaa gaataccttt tccgtacata
    63961 gttcattctc ctgttagcgg tcgagttcgc ccatcacgat atcgagcatg gccagcagcg
    64021 ctacaaagtc ggcgatcttt gtgccgatgc acatgctttc aacggcctgc aagttgacaa
    64081 aagatgcagg gcgcacatga tagcgatagg gattcggctt gccgttcgag acgacataga
    64141 agcccaactc acccttcggg gattcgatac gcccgtacgc ttcacctgcc ggaacccgaa
    64201 cctgatactg cggcttctgt gagttgatcg gcccctgggg aatctgtttg agtgcctgct
    64261 ccaaaatacg cagggactgg cggatttcat cgaaacggat caggtagtta tccatcatgt
    64321 cgccgttctg gcggaccgcc acatcgaaat caaagcggtc ataaatggag tacggatcgg
    64381 cgcggcgcag atcatacggc acacctgccg cacgaagcac aggacccgtc accgagtact
    64441 tgatcgcgtc ttccgcattc aacacgtgga tacccttgag acgcgcaacc agaatctcat
    64501 tttcgttgag gaagcgttcc atttcatcgg ttttcgcggg caagcgctcg aagaccaggt
    64561 ctttgatctt ctgcatcaca taatcgggaa tatcgcgcac cacaccgcca aagcggaagt
    64621 aattgcacat catgcgtgcg cctgaaacgg cctcaaaaat atcgagcacc agttcgcgct
    64681 cttcaaaagc gtagagcgag ggagtgtaca tggtaccgag atcgttcatc aacatgccga
    64741 tgaagatcaa gtggttctga acgcggctca actcagccat gatcacgcgg atgtattccg
    64801 cacgctccgc aacctggatc ttcatcaact tctcgatcgt ggtgacataa ccgaagttat
    64861 tgctcatcga gttgaaatag tcgaggcggt cagtgtaggg aatgacctgc agataggtgt
    64921 tgcgctcgcc aatcttgtca tggttacgat gcagataacc catcacaggc ttaagaccga
    64981 taatggtttc accatcaagc gtaacagcca cgcgcaacac accatgtgtg gaaggatgat
    65041 gcggacccat gttgaccacg atatggtctg tgttaaggct gccctcttcg ttcttcatgg
    65101 tgtggcgttc gagagaagca tacagtccgt cttcgttgac gatctcaaat ttctcggggt
    65161 cgaaatccgc ggggaatttc aaattatcgc ggaacggatt cttgttttca gcaaaggtat
    65221 gctgcccatc cggccagcgg ctcttaaaag gtttggcttc ctcttcaaag aaaggttctt
    65281 tccaatcttt gcgtaatgga tggccttcat atccttccca catcaaaata cggcgcaggt
    65341 cggggtgccc ggtgaatttg attccgagca aatcccaggc ttcacgctcc tgaaaatcca
    65401 cacctggcca gatattgatg agagacggaa tttcaacggg gtcaacacgc tccacctgaa
    65461 ccttgaacac caacccaggc ccgccggtgg tcttgtaggc gtgatagacc atttccattt
    65521 tattttcggc gatgtaatcc gcgccggtgg cggcggtgag caggtcaaaa ccaaattcat
    65581 cgcggatggc agtggcaact tctacgagat tgtctttgtt aacgataaat ccgctgtaac
    65641 cgggacgcgt atcagcagtg accttgcccg ggaaacgggc aaccagatca acggtggaag
    65701 tttggatcgc ggtggccatt atgcaccttc cttttgcgaa gcagctgcgg cctgcttgat
    65761 ctcgttgttg cggcggacat cgatcaagtc cgggccaagg atcggcatgg gcacatagtt
    65821 gccatcctgc ttttccttgt tgtaccaacg cacggtcttg atggattgtt tgtcaatctt
    65881 ttcctgcagt ttgatcaagc cagcgatcaa ggcctgcgga gtgggaggac agcccgggat
    65941 gtacacatca acgggcagga atttatcgat accagcaact acgttatagc cctccttgaa
    66001 aggtcccccg ctcgatgcgc acgcgcccat cgcaaccaca tatttcggct caggcatctg
    66061 attgtaaaga cggatgatgg cgggcagcat cttcttggtg acagtgccag agaccagcat
    66121 catatcggcc tgacgcggag tcgggcgcat cacttccatg ccgaagcgcg ccatgtcata
    66181 acgagcggtt tgggcagcga tcatttcgat ggcacagcat gccagaccga acatcaacgg
    66241 ccacacggaa gaacgacgac cccagttata caaacgatcg agcgtcgtaa tggctacaac
    66301 attttcaagg tcttcaggga tgttgtagtt cggtaaactt agcttttcat tttccatgaa
    66361 tgttctcctc gaaccagtct tgatagattg cgtaaaggat gtcatcgttc acgagcgcga
    66421 ggtcatcctc aaattcactt agttgaatcg tccattggta aatttgttga agcgaaacat
    66481 cttcgatatt tacctcaggg tgctggcggc gaagctccaa agcaatggcg taggtggatt
    66541 cccagttcaa ggtcattcgg tactttccgt cacaagcgcg aattatagca ggtgaagtgg
    66601 attagtcaac caaattacgt aaaaatatat ttgcaaaaat aaagaagtga gtataatcac
    66661 accattcgga tgacaacgag gtcatcacat aaaccgatac ctgcctcagt ttcgaaaggc
    66721 attttctccc cgatgatcac cgcccgccac aaagtcctcg cccacttgaa aaagacccgc
    66781 gccgcatcgg cacgggaaat tgcgcgcgcc ttgaaaatgt ccgcgccgaa tgtgcgccac
    66841 cacctttcag tgctgtgctc tgacgggcgc gtggaatcca cagaggttaa caaccgcgaa
    66901 ggacgtggac gccctgaaaa gttgtattcc ctgtcgcagg cggcgttggg agataatttg
    66961 tcggcgctga cgaaagccct tttgacagaa tcaggcccgc ggttaaatat cgaggcgctg
    67021 gcgaatcata ttttgaattc gggtcagttt gcaaatcttc ccatcacaaa aaggcttgtg
    67081 ctcttaatcg aaaaaataaa tgagatgcat tatcaggcgc gttgggaagc cagcgcagaa
    67141 ggcccgcggg tgatcttggg aagatgcccc tatgcaaaga tcatcgcggg gcaccccgag
    67201 ttgtgtaaaa tggatgcggc gattctgaag aatgcactgg gaaattcagt ggaacaaacg
    67261 tcgaaaatgg aaaacctgca cggcgtttgc atttttgcga tgcgataatt ttataaagga
    67321 gaattcacaa tgaccccaaa ccacctgacg aaagaagaac ttgaagctgg cctccagcac
    67381 attacacaat cacccaagga caacggtgtt gtggaaatga tcgtccgcag acccgaagtg
    67441 gacggaaggg aaattctcga atcggcagag ctgaacctga cggaaggcct ggttggcgat
    67501 aactggaaaa cccgcggcag tactgccaca cccgatgggt ctgctcatcc tgaggcacag
    67561 atcaacatca tgaattcacg tgcggttgca ctgttggcac aggaaaaaga ccgctgggca
    67621 cttgcaggcg atcaattttt tattgacctt gatctgggcg ttgaaaatct gcctgcgggc
    67681 acaaggctgg cgcttggttc tgccattctt gaagtgacgg caaagccaca tacaggttgt
    67741 gataaattcg ccgcccgcta cggacgcgcc gccgcacttt ttgtcaactc caccgaaggg
    67801 aagaaacttc acctgcgtgg gatcaatgcc aaagtggtgc aggctggcac agtgcgcgtc
    67861 ggcgatgcag taaaaaagat ctaaacctcg gcctggtgct ggaaaattac agccaaataa
    67921 aacgattttt gtttgaaatt caaccccgcc agtcaaagga gcctctcatg aaagccatcc
    67981 gttttatcgg tgccaaccaa ccccttcaaa tgcaggagat ccccattccc gaaatcggcg
    68041 agcgcgatat tcttgtgcgc gtcaaagctg cgggcatctg ccattcagat gcgcattatc
    68101 gtgcgggtat ttcacccgtc aaacctgttc cgctcacact gggacatgaa gtggctggcg
    68161 tggttgaaaa ggtcggcgca caggttggca atttgaaaat cggcgagcgc gtttgcctgc
    68221 actacaacat ctcctgcggc gattgttatc attgctccac cggcaacgac caattctgtg
    68281 aaaaggtttt gatgctcggc cactacacca acggcggcta tgccgaatac atcagcgtgc
    68341 ccgcacgcaa cgccattcgt cttcctgatg aaattccctt cgaacaaggt gccacgctca
    68401 tgtgtgcctc ggccaccgct ttccacgcac tgcgcaagag cagactcaaa gctggggaaa
    68461 ccgtggcaat tttcggcgcg ggcggactcg gacaatcggc cattcaactt gcaaaagcat
    68521 tcggtgcggt cgaagtctac gctgtggata tcaacgatga caaattaaat cttgccgcac
    68581 aatatggcgc gattcccgta aatggaaaca aggtcaacgc agtggaggag atcaaacgcc
    68641 tcacccacgg caaaggcgtg gacgttgcca tcgaaatgat cggcctgcct ctgacaatga
    68701 aacaggcttt gcaatgtgcg ggtgtcatgg cacgcgtggt catcgtgggg ctttcaaaca
    68761 aaccgctcga aattcaaacc tacacagaac tgctcggcaa cgaggttgaa ctaattggtt
    68821 ccaacgatca cttgctgcag gaacttcccc tgcttgtaga cctggcacgc cgaaaagtgt
    68881 tggacacttc aaaaatcgtc acccgcaccg tgccgctgga tgcaaatgcg atcaacgcaa
    68941 ccctcgactc actggaaaaa ttcagcggcg acgtacggac ggtcatcgtc cccgcttcag
    69001 actaaccttg ggctgattct gtttcgaaag atataatctc ccccatgaac atccccgacc
    69061 gaatcatctc tattttggaa ttacgccgca acacaggcga acgctactca cgcatgtgga
    69121 tcgcacacaa cggcatcgtg tacgatgtca ccgattgccc caaatggcgc actggcatgc
    69181 atgaatttct gcacttccca ggccaggacc tcagcggtga actcaccgac gccccgcacg
    69241 aagaagaagt cttcaaacat gactgtgtaa aaatagttgg cagattggaa agttgacaag
    69301 tttgaaggaa acaattcctc aacgttccaa ctttcaaacc tttcaacctt caaactataa
    69361 ggaataactc atgactgcac acaaatggtg gcaaacggct gttttttatc aaatctatcc
    69421 gcgctcgttc gcagacggaa acggcgacgg catcggtgac ttcaaaggcg ccacggaaaa
    69481 gctcgattac ctcgcctctc ttggcgtgga tgcggtctgg ctttctccgc acttcccctc
    69541 ccctaactgg gattgcggct acgacatttc cgattactgc aatgtcgcac ctgagtacgg
    69601 cacactcgac gatttcaaga ccttcctcaa cgaagcgcat aaacgcaata tccgcgtcat
    69661 tttggacctt gtgttgaatc acacctccga tgagcatgcg tggttccttg agtcaaaatc
    69721 cagccgcgac aatcccaaag ccgattggta cgtctgggtg gatacgccgc ccaacaactg
    69781 gcaatcctgc ttcgatggcg aagcctggac ctacgtgccc gagcgcggcc aatattatta
    69841 tcactacttc atgaagcaac agcccgattt gaactggcac aaccctgaag tgaaaaaagc
    69901 catgtgggat gccgcccgtt tctggctcga tatgggcgtg gacggttatc gccttgatgc
    69961 cattggcacc attttcgaag acccgcaact caccccgcac aatgtcccaa tgaatttagc
    70021 ggaattgcgc cgtttctctg agcttgccaa cacgccaaag gagaaaaagc tcaaagacaa
    70081 atactggtat gaaatgttca agcatcaata tgggaaacca ggtgtacatg agctaatgaa
    70141 agaactccgc gccattctcg aagaatacga aggcgaccgc atgctcgttg gcgaagatga
    70201 cgacatcgcc tacatgggca acgggtggga tgaactgcaa ctcatcttca acttccccct
    70261 catgcgcaca gaccagatca cgcctgcaca catccgcaag aaccaaaagg atagacttgc
    70321 cgctctggaa aaactacccg tcgaaacagg ctggccatgc aacacccttg gaaatcatga
    70381 ctgctctcga atccacaccc gctatggcga taagatccac gacgcagaac tggcacgttt
    70441 gaacgccgca ctcgttctaa ccctcaaagg aacggcattc ctgtacaacg gcgaagagat
    70501 cggaatgacc gatttcatgc tctctgatat ttcacacgtc aaagacacca tgggctcctg
    70561 gtattaccac accattgtca ctgaaatggg tgtccaccct gaagaagcca tgctccgtac
    70621 cgcagaaatg actcgcgaca agaaccgcac tcccatgcaa tggtccaaca gcccgaatgc
    70681 aggcttctct ccgtcaggcg tgaatacctg gctgcctgtc aacccgaatt acaaagacgg
    70741 catcaacgta tacgaccagg acaagaaccc aagttcactg ctgcactact acaagcatct
    70801 gttgcgtgtg cgcaagggtt ccccggcatt acagcatggg gaatatcttc cactccacaa
    70861 tacctccagg gaatatttcg ccttcctgcg ccgttctgaa aaccaaacag ttctggtggt
    70921 cttgaattac tccgacaaag tatttgaact tgatttttca cgaacaaagg agatcaagga
    70981 ttcaaacctg cgcatcctgt tcaccagtgc agaaagacgc ggcggatcat ttgcacccag
    71041 attcaaagtg ggcgcattcg aagtgttcat cgcggaaaca gagcgtggat aaagaaaagg
    71101 caaaaaacag caaaaaattc gtggcgctgt gcggaagaat attccgcgat gccgacccga
    71161 aatatttccc gtccgcacaa tatcgcgggc gtacacttcg cttttgcaca gagtcctgtc
    71221 tcggcgcatt tttagccgat ccagatattt tctacaaagt ccaccgcaac tcggaaaaaa
    71281 acaaagatgc caaaccttaa tttgtggaaa gcaggcgatc acatcctcct gcgtggtgtg
    71341 tacaatggct caccaaccta catgcaatca gcacgtgtgg tacaagacac ccccgaagaa
    71401 accgccctgc tcatttggcc cggcgcagaa tgcatggttc ccagcggata cctccaccac
    71461 gcccatgaca ggtccatgtg ggatcgctgg gcagaaacaa ccacaaacac cttgaaactt
    71521 gaaaaataca tctggcacac caaccgattc ctgattctgc ttgaacccga aaaatatttt
    71581 tccacgatct acatctggga agcagcaaag gatgagtttg tggggtacta catcaatttt
    71641 caactgccct accgccgctc acacctcgga ttcgacaccc tcgaccttga cctcgacatc
    71701 gtcatcgaac ccgatttcca gtggagctgg aaagacatgg acgaatatca acaaggtatc
    71761 gaggctggcg gaatacacgc ggactgggtc aacgagatcg aacgtgccaa acaggaagta
    71821 ttcacccgcc ttgaaaaacg cggataccct tttaattcag actggctgaa ctggcgtccc
    71881 aacccgaact ggaccgcgcc cagcctaccc gaaaactggg atgtgatcga ttaacggtat
    71941 acggagtttg agatgaacgc cccacaaaaa tcaatccact atgagcagta cgaattccaa
    72001 aaatggaaat ccttcgatgc agagacgatc gttgaaatgc ccgtttcttt gacggtgaac
    72061 ggcaaagtat ggatcagctt catgtgtacg cccgtcaacc tggaggcgct ggcagttggg
    72121 tttctcttca acgagggcat tattcaagaa atggatgagg tggaagatgt ccgcgtgtgc
    72181 gagcacggtg acaatgtgga tgtatggcta agtcatgcgg cagaacagcc aacctcatgg
    72241 agacgaacct cagggtgtac aggcggggta acagcagtgg atcttctcgc gaagccgaat
    72301 gtctctttaa gcgggaacag cttcacggtc aggcctgatg cgatcggtca gttggtggaa
    72361 atgcttttcg agtcgcagtc cctgtatcgt gaaacgggcg gtgtgcatac atccgcttta
    72421 tctgatggtg aaaaaatcct gatcgccgcg gaagatatcg gcaggcataa cacgttggat
    72481 aagatcgcag ggttgtgcct gatgaatgat atctggcctg aaaatcgaat tttgatcacc
    72541 acaggccgta tcagttctga gatgctgcaa aaagcagcgc agatcaacgc gccgatcctg
    72601 atctcgcgca cttctccatc ttcgctttca atcgagatgg ccgaacgcta cggaattaca
    72661 ctgatcgggt atgcaagaaa acaccgtttc aacgtgtata caaatggcga aagagttggg
    72721 ctataaaagg aggaatacac atggctaaaa aagacagatc gaacggaacg ccaatttcat
    72781 ttcaaattcc gtcaagtgga gggaaaggac ctcgcatccc catccccacc aaccagcaaa
    72841 agtggaaata ccaatctggt tacatggcac ggcccacccc caaagaatgg atcgctgccg
    72901 tcattgcttt ttttgttatt gtaggaattg gctttttgct ttctgtcgaa tcgtgggcgc
    72961 ttacggttgc attttgcgtt ttcgcaatga tcataccggt tagttttata caaagggaaa
    73021 tgttcttccg taaaagcaga aaggatcagg caaaaccaag gagataggac ggttccaaat
    73081 ttccatccga aaacactttg aaaccatatc acggaaaaag acataaaagc cacccaatac
    73141 tgatgagtgg cttttttctg ttatacttga ttgtgaaaat tgtcacaaca agaaggataa
    73201 tcatctccac gaattatgag taatgaaact taccaaagga gataagctag agaataattt
    73261 aattaaagat tatccattga aaagcaaatc agaaaggtat gactttaacc atgaatctga
    73321 atcaagtgat cgaattggta ggcggcacaa cccttacaac cgaagcggac ctgaacatca
    73381 atattcaagg cggctatggc gccgacctga tgagcgatgt ccttgcagcc agccaaccca
    73441 acgccgtttt attgaccggg cttaccaatc cacaggtggt gcgtaccgcc caaatggccg
    73501 acttccgcgc aattattttt gtacgcggta aacagcccca ggctgaaacc ctcgaacttg
    73561 ccgaacagga aaacatgcca ttaatcacca gcccgctagg aatgttcgaa ttgtgcggcc
    73621 gcctccacaa ggccggactg ccgagttttg aatccaatgt gtcctaacag aatccgcacc
    73681 gaacaggaaa tccacggtcg gcatggacgc cccatcactg atcaggatgc ccacgaaatc
    73741 aaccgcgtgg aagaattgtc ctatgacctg aagatccacg aggtgatgtc aaaaaatttg
    73801 caaactgcca gcccggatat ctccctatcg gatgtcttga agatcctgcg catcaaccgc
    73861 atttcaggcg tccctgtagt tcaggacgag aaactggtcg gcgtgatcag catcgaagat
    73921 attgtccgcg cactgcaaaa caatgacctg acggaaaccg tcggacaata tatgacacgc
    73981 gagcttgtca ccgtggcaag tttcgactcc atcgtcaaag ccatgcaaac ctttgccgaa
    74041 aaacacgtgg ggcgtcttcc tgtggtggat gaagaaaaaa aactcgtagg gatgatcacc
    74101 aaaggcgata tcacccgcgg cattctggtg gcgctccaaa aagattacaa agcggaagaa
    74161 gtgcgccgct atcgagccag tcacctcttc gaagatatca tctccgaccg caccacactg
    74221 gtgctgcgct acaacatcaa agcccaggat tttacacacg gaggcaacgc ttccagccac
    74281 atcaaacgcg ccctgctgcg acttggcgcc gatgcacaaa ttgcgcggcg ctgcggcatt
    74341 gccgtgtatg aagccgaaat gaatctcatc attcacacca cccatggcgg cattctcaaa
    74401 cttgaagtgg aaccgcaccg catcaccatg tccaccaccg atgacggccc aggcattccc
    74461 gatgtcgaac aggtgctgca gcctgggtac tccaccgcaa ccgaccaggt ccgtgaaatg
    74521 ggattcggcg caggcatggg gctggtcaac attaaacgct gtgtcgacaa catggaactc
    74581 gaatcaacag tcggcaaagg cacaaaactc atcatgcgga ttcatgtgcc tgccgaaaat
    74641 ttcaacgcac gcaaggaagg atgactcccc atgaaccttc aacaaatcat cgaacgactc
    74701 aacctcaatc tgctgactga tccacgagat tttgaaacga tccaccccga aggcgggtac
    74761 acctcagacc tgctttcatg tgtcatggca ggtgcaaaaa gtaaatatat ctgggtcacc
    74821 ttgcaggcac atttgaatat cgtagccgtt gccgccctgc tggatgtagc cgcgatcatc
    74881 attacagaag atgcacagcc agatgctgcc acgatcacaa aagccaacga acagggcgtg
    74941 atcctgcttt ccacttccat gcccagcttt gaagtggatg gcaaactttg ggaaatcggc
    75001 ctccgcaatt aaacactgac cggcatgaaa aattttcgcg ccgacctgca cgtacacact
    75061 gtactttcac cctgtgcaga agtggagatg atcccaccgt tgatcgtgca ggaagcgctc
    75121 gaacgccaga ttgatatcat tgccatcaca gaccataacg ccagcgcaaa cgtcgctgct
    75181 gttcaaaaag cagcgcacgg aaccgcacta cacgtattcg ggggaatgga agtgcagacc
    75241 cgtgaagatg tccatcttct ctgtttgttt gaccacacag aagcactcgc caactggcag
    75301 gccctggtag attccgcact gcccaacctg aaaaacaacg ccgaattttt aggggaacaa
    75361 ttcattgtgg atgaagaggg caactttatt cgcagtgagc cgcgcatgct gcttacgtcc
    75421 acgcattttt cgatcgacga aatcttcaat aaagttaatg cgttaggcgg actcgtcatc
    75481 ccagcgcatg tggataggaa cagctacggt cttttcccaa ccttgggcct gctatcgccc
    75541 aattggccaa ttctttccct tgaaatttcc cgccacatct cccctgaaaa agcagcctct
    75601 ctctacccat tcatcaaaaa cactcccctc atccaaagtg gcgacgttca caggctcgat
    75661 gaatttcttg gcaaaacgat ctttacaatc gatcaaccat cgttggcaga aattcgcatg
    75721 gcttttcgaa atgaaagcca gcgaaccgtc aatatcgagc cgtaataatt tgactgacaa
    75781 ccttcaccag tctttatatg acattggtca ttgacgctat gaaactgtat gataaactag
    75841 atttgtgaaa attatcacaa tatttatcga aggtaaagcc aatgacgcaa tcactcaagg
    75901 aaatcccagc cactgaccca ctcagcatcg cggaacaaaa acagatcata gatactgcga
    75961 ttttggaaaa caaagaccgc cccggttctg taatgctggt gctcaatgaa gtacaaggcc
    76021 aaatcggcca cgtctcccct tccatgcaag cctacattgc ccgtcaactc ggcgttccgc
    76081 ttgggcaggt gcatggagta gtcactttct attcgttctt caaaaccaaa ccgcgcggca
    76141 aacataccat caagttttgt cttggcacag catgctatgt ggcgggggtt ccacagttga
    76201 tcgaaaaagc caaacaaatg ttaaacgtgg agttgggcga aaccaccccc gatgggcaga
    76261 tcacgttgga agaatgtcgt tgtgtgggag catgcagtca ggcgcctgtt gtggtggtgg
    76321 acgaagaagt gcagggacgc ctgcgcccga acaagttccc ccaggttttg aagaaggtgc
    76381 aaaagccgtc atgagagaga ttgccctgca tctaatggat atcgcagaga acagcgtcgc
    76441 cgctggcgga cgcaacatcc gcatcgctgt ccacgaagat ttgcaaggtg acctgctgac
    76501 agcaacagtt tcagacgatg gtcgtggcat ggatgaggaa accgccagac aggtaatgga
    76561 tcccttctac accacacgca ccacccgcaa agtgggtttg gggattccgc ttttgaagct
    76621 ggcggcagaa atctccgggg gcagattaag cctggtaaca gaagcaggca aaggcacaaa
    76681 gatcgaggcg tgtttccgtc acagccacat tgaccggatg ccgctcggcg acctcgcatc
    76741 cacatttctg gctttgctgg tgtcctatcc gaatattcat tgggtattcg attacaaaac
    76801 cacagacaaa aacggcaacg acgataactt tgaattcgac gatgccgaat taaaaaacga
    76861 actgggcgac atgtcgctca ctgaacccga aatcttgaag tttgtacgcg ggcttatcga
    76921 agagggcatt caagcctctg cgctgcaaac aaatttctaa aaggagaaca tcatgcctgc
    76981 gatcaaatca ctggaagaat tgaagcgcgt tcgcgaagag gccttgcaaa agaaaaaaat
    77041 gaaagccgcc ccgggcaatg tgcaggtgat cgttgccatg ggcacctgcg gaattgcagc
    77101 aggcgcacgc gacacgatga agagtgtgct gaactatatc gaaacgaata acctaagcgg
    77161 catcactgtg acgcaaacgg ggtgcatcgg tatgtgtgag caggaaccga tcgtacaagt
    77221 ggtgatcggc gatcaaccca aagtagttta cgggaaagtg aatgccgaag tggccgggca
    77281 gatcatgaag cagcatgtgc aaaatgggca gcccgtaaag gattacgttt taccgagcta
    77341 gctgtgtaac ggccatggcc gttattacaa gatcgagttt tggagttttg tatggcgttt
    77401 tatcgttctc atgttttggt gtgtgttgac cctgagtgtc ttgcaaaagg cgctcatgaa
    77461 gttgttgacg cattacagga tgaatttgtc gcacaggggt tgattgacga gatccaggta
    77521 ttggaaacct cacgcatcgg cggatgtagt gacggccctg aaatgatggt gtaccctgag
    77581 ggcgttcact atcacggcat gaccgcggat gacattccat ttctagtgga agaacatttc
    77641 ctgaagggcc gcatcgtgga gcgcttccgg gaagtgacca agcctgtggt ggatgaagaa
    77701 cttggtccac tgcgcccgaa ggaagtgcgg gttgtactgc gcaactgcgg tgaaatcgac
    77761 ccgaacaata ttgaagatta tctggcaaca gatgggtatc aggcgcttgg caaagtgttg
    77821 tttgaaatga cacccgaaca ggtcattgaa gaagtgagca attccggtct gcgcgggcgc
    77881 ggcggtgcgg gtttccccgc cgggagaaaa tgggggctca cccgtcaggt gcaggataac
    77941 cccaaataca tggtctgcaa tgccgatgaa ggcgacccgg gcgcattcat gaatcgccgc
    78001 gtgcttgaag gagatccgca ttcagtgatc gaaggcatga tcatcggcgc gtatgcggtt
    78061 ggcgcaagtt atggatacat ctattgccgc gccgaatacc ccgtggcagt ggaaacgctc
    78121 aacattgcca tcaaacaagc acgcgagttt ggtttcctcg ggaacaacat cctcggcacc
    78181 gatttcaatt ttgatcttga agtgcgtgtc ggcgcgggtg ctttcgtctg tggtgaagaa
    78241 acagctttga tggcttcgat catgggcatg cgcggcgagc cccgtccacg cccgcccttc
    78301 ccttctgtaa aaggtgtgtg gggtaaaccc agcaacaaca acaatgtgga aacttattcc
    78361 aacgtgccgc agatcattct aaaaggcgcg gcatggtatg ccagcatggg cacggaacga
    78421 tccaaaggca caaagacctt tgcggtcggc ggaaatgttg tcaaaccagg tctcattgaa
    78481 gtgccaatgg gcatcaccct gcgcgagatc attttcgatg tggctggcgg aatcaaagac
    78541 ggcaaaaaat ataaagccgt acaaacaggc ggcccaatgg gcggatgcct gaccgaagaa
    78601 catcttgacc tgcctctcga ttacgaagca ttgactcaag cgggctccat gatgggttct
    78661 ggcggccttg tcgtaatgga cgaaacctcc tgcatggtgg atatcgcccg cttcttcatg
    78721 gattttacac aggcggaatc ctgtggaaaa tgcaccccat gccgcgtcgg cacacgccgt
    78781 attcttgaac tgctgcaaaa gatctgtgat ggcaaaggcc aggatggcga catcgaaaag
    78841 cttgaaaacc tttgtcacga agtggcacgc aatagtttat gcgggcttgg tcagggcgca
    78901 cccaaccctg tgcttagcac cctcaagcac ttccgccacg agtacgaagc gcatatctat
    78961 gaaaagcgct gccctgcaaa agtctgccgc cccttgatcc attacgaaat tcaggcgccg
    79021 acctgcacag gttgtaccgt ctgcgcgagg aattgtcccg tggaagccat cagcggtgag
    79081 agacgtcagg cgcatcatat tgaccctgac acctgcatcc gctgcggaat ttgtatgcaa
    79141 gtctgtaact tcaacgcaat tgctgtcgaa tcctaaatcg aaatcgagaa tcatgtgatc
    79201 ggagaaggaa tgactgcccc aaccattgaa agagacgtcg tctcggtcgt ccaggctgca
    79261 atcgagaaac atggcaacgg acgggatgcg atgatccccg tcttgagcga gatcaaccgc
    79321 caatttggat atgtgccccg tgaagcgatc cgtgaagtgc gcaagcagct ccacgatccc
    79381 gcccgtcagg tgcatattgc cgaaagtcaa ctctacacgt tgaccagttt ctacgacatg
    79441 ctttccacca aaccacgcgg acgccatgtc atcaagtttt gcgagaatgc accctgccat
    79501 gtggtgggcg gaaaagccat ttgggatggg ttgagggaga agctcaatct cgaaaacgga
    79561 gaaaccaccc cagacaacaa atggactttg gtaaccactt cctgccttgg tctatgcagt
    79621 gtgggccctg tcttactcat tgatgatgac atgtacggca atgtcacccc tgagcagatc
    79681 acagagattc ttgaaaaata cgaataagag aggccaacat gattaagaaa cgagccatga
    79741 tcctggtctc caacgacccc gaaagtatcc gcctgggcgc caatgaaatt gtgcgtcatt
    79801 tgaacgaagc cctcgtcacc tacggactgc aggatgaggt ggatgtttcc accgtctcgg
    79861 atgtcaacca ctcgaatatt cttccgctgg tggtggttta cccggaagca gtggtgtacg
    79921 gccctgtaaa agctgcagat gtgcgctacc tggtggaaga acatttatac aagggacggg
    79981 ttgtcaatga cctgctggca cctcccaaaa tgctgaccgg ccagatcggc tggctgcgcg
    80041 gacacaaagg ctacaacccc gccgaacagc gcattgtgct ggagcgtgca ggccgtatcg
    80101 accccgaaag cattgaggaa tacatcacgg caaatggtta cgaagccctt ggaaaagtac
    80161 tgggagaaat gacacctgaa ggcgtgatcg acctggttga gaaatcaggt ttgcagggac
    80221 gcggcggagc gggcttcccc accgggcgca agtggaagtt cgtccgcgct gctgcgggga
    80281 gcaaaaagta tatcgtctgc aatgcagatg agagtgagcc cggaacgttc aaagatcgca
    80341 ttgtcctcga aggcgatcca cacgtcatcc tcgaagcgat ggcgattgcc gcatatgcac
    80401 ttggcgccga tgaaggttat atctatattc gaggtgaata cggacttgcc tatcgacggc
    80461 ttgaaaaagc cattctgcag gcaagggaat tcggctttct cgggaaaaac atattcggca
    80521 caaatttcaa cttcgatatt cacatccacg ccggggcagg cgcgtatgtg tgcggtgaag
    80581 agaccgccct gctcgaatca ttggaaggca aacgcggtga accacggtca cggccgccct
    80641 accccgttac cagaggtttg tggaacaaac ccacatcagt taataatgta gaaactctcg
    80701 cgaacgtccc tgccattatc aagaatgggg ccgaatggta tcgctccatt ggcacgcctt
    80761 cgagcccggg cacaaaggtt tacaccatca tgggcaatgt caatttcagc ggtgtgatcg
    80821 aagttcccat gggtgtgacg ttgcgtgagg tcatcaacat ctatgccaag ggaatgaaac
    80881 ccggcagcac gctcaaacta gcacaaacag gcggaagcag cggctcgatc attcccgctg
    80941 ttttgcaaga tacacccatg gattttgaat ccttccgcaa ggcgggtgtt tcgcttgggt
    81001 cgggcgcatt gctcatctgc gaccagaaca cctgtatcgt ggaccttgtc aaagtgctgt
    81061 tgcaattctt ccgctttgaa tcctgcggaa aatgcacccc ctgccgtgtc ggcacacagc
    81121 gcgcctatga gatcgtggat cgaatttcaa agggacaagg caggctggaa gaattggata
    81181 cattgctcgt tctcgccaac gaaatggaac gtgcctccaa ctgcgggctt ggacaaaccg
    81241 ccaccgtgcc gatccgcgat atgctcaaac atttccgcac cgaagtggaa gctcacatcc
    81301 gctctggcag ttgtccgact ggtgtatgtg aaatggcaca ggaaatcgag aaggtttaaa
    81361 acctttcatt caaaaaatag ttgaaaccag aacttcaaca cagaggtatg tatggcaaaa
    81421 ataacgatta acgaaaaaca gatcgaagtg cccgatggaa caacggtttt gcgcgccgca
    81481 gaaatggcag ggatccacat cccacgcctg tgcgaccaca aggaactcac tccctatggc
    81541 ggctgccgcc tgtgcatagt ggaagtggaa ggcgcacgtg tgcccatggc atcctgcaca
    81601 ctaccggtaa acaacggcat ggtggttcgc acaaacaccc cagccttgaa gcaatcccgc
    81661 gaattcatcc tttccatgct gttcagtgaa cggaatcatt tctgcccgtt ctgtcaggtc
    81721 agcggcggcg attgcgaact gcaaaactca gcctaccacg aagaaatgac ccactggccc
    81781 attcaaccag gctggagcaa tttcgcagtg gataccaccc atccatactt tattttggat
    81841 aacaaccgct gcatcctttg ccgaaggtgt gtgcgtgcct gcggagaact ggcaggcaac
    81901 tttaccctct ctgttgcaga acgcggcgcg gccagcatta ttgttgccga taccgatgtg
    81961 ccgcttggtg aaagcacctg catcaaatgc ggttcctgtg tacaggtctg tcccacaggc
    82021 gcattgatcg accgagtcag cgcctaccag ggacacgaca aacacctcga agaaatcaag
    82081 tcggtttgct caggctgttc agtgggatgt tcgatcaaga tcatggtacg cgataaccgc
    82141 atcgtccgca tcgaaggaga ttgggacggc aaggtcaatc gcggcgtgtt atgtgagcac
    82201 gggcggtacg accccgtcac agaatctcgc aaacgcatca ccacaccgct ggtccgcaag
    82261 aacggcaaac tcgagcccgt ctcgtgggat gaagccatgg gcgctgtggc aggcaaatta
    82321 atggaaaaca aaaacagcat cgcggccatt gcctcaacga gattatccac tgaaaccctt
    82381 tccgctttca aagaactgtt ctctgaaaaa ttccaaagca aactggtcac cagtgtggaa
    82441 gaaggcgtac ccaccgcgtc tgtcacccgc tttgcacaaa cccacgaagc cttcgaaggc
    82501 aagctcgatg ttttacgcaa tgccgacacg gtaatgtgca tcggtgcgaa tctgcatcgc
    82561 agtcacatgg tggcgggctt catgttcaaa cgcaatcttt ccaagggaac acgcctgatc
    82621 accattgacc ctgaatcaag cgaactggat tacatggcca atatcgcttt gaagacccaa
    82681 cccggctcag acctcgcact catccgcgga ttgcaagccg tcatcgtcca ggaggaattg
    82741 gggcgcgcgc ctctttccct acccgatgca ggccagctca tcgaaaaagc cgtcagcgcc
    82801 tcgggtgttt ctctcgaaga actcaggcag acgggtcggt tattggcgcg ctccatttcg
    82861 ccagtgatcc tgttcggaaa aggcgtcact gcccaacgtg atgaaaccat cgttgaagaa
    82921 ttgcatcgcc ttgccgtgtt gatcggcgca gtggatagcg aacgccacgg actgctctcc
    82981 atcaaaggtg aagccaacag cctggccgcg gccctgctca gcatggacag cgccttcgaa
    83041 ctgaatggag aaaaagttgt gtatgccgcc atcggtgacg actacatctc caaatctctg
    83101 gtggaacggg tttccaaagc gccgcatctg gtggtgcaag ccagttacga atccaaactg
    83161 accgaacaag cagacgtcgt tttacctgtc accatttggg cggaacagga agggcactac
    83221 atcaacctgg atggacgaat tcaggaaagc cacaaggctc tggtgccgcc cgaaaccgtc
    83281 cgtgaaaacc tcgccatctt gaatgaactt gcgatgcgaa cgaataccac catcaaaacc
    83341 gattggcaaa aagccattct ggcacgcaga tcaagcgttg cattgaatta attgccaggg
    83401 aaaagagtct ctcatgaata aaccaaagat cgcttcagac tggatgtgcg gctgcgctgg
    83461 gtgtcacatg tcattactgg atatggacga acgcatttta cagatcgtgg aacttgccga
    83521 cctgcgagcc acccccatca ctgacctgaa agaacccgat gaaagcggcg tggatgtagg
    83581 tgtgctggaa ggcggcatca acaacagcgc caatgaagaa gtggcacaca aaatgcgcca
    83641 acgcgccaaa atccttgtag cgctgggtga ttgtgctgtg tttggcggcg tacctgccat
    83701 gcgcaatctg tgcggcgtgg aagccgcact gcgccgcgct tatgtggaag ctgaaagcaa
    83761 cgaaccaaac agccaaattc ccaacgaccc cgaactggca accatgacca aggcacgtgc
    83821 catccatgaa gttgtccctg tcgattacag cgtgccaggc tgcccgcccg atgcagacac
    83881 catcttccac gtcctcgccg aattggcgca ggggcgtaag cctgaaatta aagacgacct
    83941 attgcattgg cattgaatta tgggttgcag gttgaaggtc aaaggttgaa agttgaagga
    84001 tttcctgagt tggagaaaaa tatgagcaca caaaagatca cgattgaacc cgttacccgt
    84061 atcgaaggtc acgcaaaagt caccattcat atgaaggcag ataacacggt tgaccatgcc
    84121 tacatgcatg tcaacgaatt ccgcggattt gaaaaatttt gcgaagggcg cctgtatttt
    84181 gaaatgccgc agatcactcc gcgcatttgc gggatctgcc ccgtcagcca tcatcttgca
    84241 gccgccaaag ctggcgatgc gctcacaggc caaacccctc cacgccctgc caatctgttg
    84301 cgcgaaatga tgcacatggg tcagatgatt cagagccacg gaatgcactt ctttgaactc
    84361 gctggccccg acctgttgct tggcttcgat gcctctcctg aaattcgaag tgtcgttggg
    84421 ctgatcggcg ccaaccccga actcaccgtc aaagccgtgc agcttcgcaa gtttgggcag
    84481 gaaatcatca agacgctcgg cggccgcaag ttacaccctg tctttgcggt gccaggcgga
    84541 gtcaacaaag cgcttacgat ggcggaacgc gattccatcc tcagtggtgt ggatacggcc
    84601 gttgccactt tgcaggcagg cttgcagatc atgaaggact gggcgcaaaa gaacatggaa
    84661 gacatcaaca agttcgccgt gttcccaaca ggctatttcg gcctgaccac ccctgaaaac
    84721 ggtcttgaac tatacgatgg caatatccgc ctcatcagcc gtgaaggcgt ggaaatggag
    84781 cgcttcccgg ttgccaatta tctggatgtg atcgccgaac atgtggagcc gtggtcgtat
    84841 ttgaaattcc cctactacaa aaagatgggc taccccgatg gagtgtaccg cgtcggtccg
    84901 ctcggacgcc tgaatctggc cgaaaaaatt gatacgccgc tggcagatcg tgaactgaag
    84961 atcttcaaga gcctgaacaa tggcaagcct gttgaaaaca cactctacta tcactacgcc
    85021 cgcctcattg aagcgttgtt tgccgcagag cgtgtgcgcg tgttatgtga agaccccgat
    85081 atcctcagca ccgatatcct caacactcgc aggaactaca caggccacgg cgtgggcgta
    85141 ctcgaagcac cgcgcggaac cctcatccac gattacactg ccgacgagaa tggcaaactg
    85201 ctcaaggtga acctcatcgt ttccacaggg cacaacaatt gggccatgac ccatgctgtg
    85261 gatagtgtcg ccaagaccta cgtcaaagga ccagacgtcc atgagggaat gctaaatcgt
    85321 gttgaagcag ccatccgtgc gtatgacccg tgcctgtcat gttccactca tgccgtcggg
    85381 cagatgccca tccagttgga tatcgtcgcc gctgacggca cactggtcaa gaccttgaaa
    85441 cgagactaga atatttttac ccattgaagc gcaaagccag ctcaatttta gaaatattgt
    85501 cgctcatcgt tcctcaaatt ctgtcaaagg agtccaacca tgacgacagt tagaaaaata
    85561 gttgaagaaa aagccgagac caaggttcac accattgccg ccgatgccac cgtactggat
    85621 gcgctgcagg tgatgaacga ggccaataca ggcgcagtac ttgttacaga gaatgaaaag
    85681 atcatcggca tcttcaccga acgcgactat gcccgtgaag gggaagtgaa ggggaagttc
    85741 gcaaaggaca ccatcgtccg cgatgtgatg accagccaga tggtcatgat caagctggaa
    85801 accaccctgc aggaatgcgc ggaactgatg cgccaatacc gtgtgcgcca cctgcccgtg
    85861 ctcgataacg accgtgtggt tggcatggtt tcgattcgca ggctggccga ggcgctgctg
    85921 caggaacaaa aaggcaccat cgttgagttg gaaaactaca ttctcggtac gggctacggc
    85981 gagtagcccc aactggccga cattttaaaa agcaaaggta gaacggcggg ccattctggt
    86041 ccgccggaat atataacttg aaaaaaacac tgatcatagg gtatggaaac cctgaccggg
    86101 aagacgatgg ggtggcttgg catattttac gagcactgac cctaaagctg ggactgcccg
    86161 cccctgcctc ttatgaggac gaatttccgc agaacggggc attcgaattt gcatttcatc
    86221 tgcaattgac acctgaaatg gccgaagata tcgcggcttt tgaacaagtc tgctttgtgg
    86281 atgcacacac tggcagcatc ccagaagaag tacggttgat cgaagtggag agtgatttcc
    86341 agcgttctcc ttttacacac catctcaccc cgcaatctct ggtgtcgatc tgtgagtcgc
    86401 tttatcagcg caaacccgag gctgtactgc tctcggtgcg cggctatcaa tttctttttt
    86461 cgcggcagtt atccgctcaa accgaagcgc tggtcccgca ggccgtccgc ctgatctgga
    86521 attggttgca aatgtcatag acatcaatca ctagtttgat ttcatgccat tgtttctttg
    86581 ctatactgac agcagtcgta acaaccaaaa ccatttaaag gagtgacacc catgaccact
    86641 gtccgtaaac ttttggaagc gaaggaaacc tcaaaaaact actcaattgc atccaccgat
    86701 accgttctga aggccattcg attaatggcc gagttgcaca tcggcgccct gttggtgacc
    86761 gatggcggga agatcgttgg catttacact gaacgtgatt atctttacaa aggcgaaaac
    86821 gaaggccgtg ccgcaaaaga caccttactc aaagatgtga tggtctccag tatgatcact
    86881 gtcaccaaag acaccagtgt ggagcagtgc atggcattga tgaaacaata caacatccgc
    86941 cacctgcctg tggttgagca cgaccagttg gtgggcctgg tctccatgcg cgatgtgatg
    87001 ttcgcagccc tggaaaacaa ggaaagcgag atccgtggat tggaaaacta catcatgggc
    87061 tcggggttcc agtcgtaagc gtttcggtaa aatcggttta taaaagaggc gcatccatac
    87121 ggtttgcgtc tctttttcat tgcaccatat cccactcttt tcctctccgc tggtaaaatc
    87181 gcagccatgt ctgttcagaa aaaatcgtct ttcaagtttc ttttccgaag cattattctc
    87241 catttgttgg cggccatcct tgtttcatgc gcacctgcca cagcaacacc agccgcccac
    87301 cctacctacg atcctttttt gccggtttcg gaggggacat ttgcgctttc tcccaatatc
    87361 cccacagtca ccattaccag cacccctgtt ccgcctaccg caacgcggga gccgaccccg
    87421 acacgggttc cgttgacgat catcctccca accgcagttc ccaccgacca gccaatcgtc
    87481 acacccactc ccgatgcgga acgtgtcctc ccaaccccaa gacaggatga ggcgcaatac
    87541 gtggtccaat ttggcgatac gctgggcggc atcgccttca tgtatgggat cagcgtggaa
    87601 gacctgatgc tgtccaacaa tctcactgat gcaaatatgc ttgatgtcgg catgaccttg
    87661 aatgtccctg cgcccaaccc cagcgaaggc ggaacatctt tcaaagtgat tcccgactca
    87721 gaaatggtgt acgggcccgg cagtgcctac tttaatattc aggaattcat cgaaagccag
    87781 aacggttatc tggaaatcta tgaacaggat gtgagcggat tcatcctctc aggatatgaa
    87841 atcgtttgga tcgtcgcccg aaattactcg atcaatcccc gcctgttgct ggccctgctc
    87901 gaatatcaaa gcggatgggt caccaaccct gaacccttca acaccgccta ccccatggga
    87961 ttggcagatg aaggccatta tggcttgtat cgtcagctca cttgggcggc aaacaccctg
    88021 aaccaaggct attatctttg gaaagcaaat gccatttcaa cctgggttct gaacgacgga
    88081 acggttgtcc cggtggaccc aaccattaac gcagggacag ctgccgtcca atacttcttt
    88141 gcccaactgg atgatcgacc cgtttgggag caggacgtca gtggaacagg ccttttgctt
    88201 tcttattatg tgttctttgg caatccattc gatttcgcca ttgaacccct cgtacctgca
    88261 tacgtttcac aacccacaat gcagctcccg ttcagcgaag gcgaaacctg gtatttcacc
    88321 ggcggcccac acggcggctg ggattacggc tcagcctggg ccgcgctgga ttttgctccc
    88381 cccggcgata atggcggttg tggggtcagt ccgctttggg tgcgggctgt ggcggatggc
    88441 cttattttgc gtgcctccaa cggcgctttg atccaggatc tcgataatga cggttatgag
    88501 caaacaggct gggtaatcct gtacatgcac gtcgcagagg aagaccgtgt ggagcccggt
    88561 gagtatgtct atgcgggcga acccatcggc catgcttcat gtgaaggcgg aatctcgaac
    88621 gccacccatt tacatcttgc gcggaaatac aacggcgagt ggatcgctgc ggatggtccg
    88681 ctgcccttca acctcgatgg ctggatctca agcggcggcg gcagggaata tgatggctat
    88741 ctctcacgca acggaattac aatcgaagct ctcgatagtg ccaacgaatt aaatcaagtc
    88801 tatcgttaat tcatctgaac ttaatccagg cggatataat cttaaccatg tctcgcacac
    88861 ttgccattct catcgtacta tcgctggttt tatcggcttg catgtcttct tcaccctcac
    88921 tttggggggt tccacaaacg cccacgccca acaccgtgga tctggcttca gcacccattg
    88981 acccatttgc aatacaggat catccgatcg acttccctac ttcaaccgtt ccgcctgaga
    89041 tcgccacaga aaacgcctat accccaaccc caaccatcac gccaaacagt tccacgcctg
    89101 tcattgaacc gacctacaca ctggcctatg atgcagcccc cttcctttac tatgcccaaa
    89161 gcggcgacat gctctccgct gtggcaaacc gttttggggt ggacgaatcc gagatcacct
    89221 cagatgctga cttgaccaag accaccctga ttgaccccgg tacgctgctg gtcatcccca
    89281 atcgcatcac tgaaccgatg acaccctatg tgcagatcat gccggatgcc gagctcattt
    89341 tctcctacac ggcggttgat tttgacgtga aggagtacgt tcaacaatcg ggcggatatt
    89401 tgagcaagtt caaggattat ctcggttcca caggctggat cgatggcgat gccgccattg
    89461 aacggcttgc caccgaaaat tcgatcaacc cgcggctgat cctggccatg cttgaattcg
    89521 aaagccgttg ggtacgcgga caacccgtgg acgaattgca cattgattac ccgatgggat
    89581 tcaacgacta tcactacaaa ggcatgtcgg ttcaattgac gtgggccatc aacaacctcg
    89641 ccatcggcta ttacagttgg cgcgcaggca cattgaccca tttggaattt cccgatggca
    89701 ccaccatgcg cattgatccg cggttgaatg ccggcacggt ggcaattcaa tatctgttct
    89761 cacgcagcca cagtccttcg cagtgggaac agatcatcaa tccgtcatca ggctttcccg
    89821 ccatgtttaa tgaaatgttc ggtgacccat gggcacgcgc cgatgccgtg aaccccattt
    89881 tcccctctgc attaaaccag ccgccattgg ttctgccctt tgagcctgat gtggaatgga
    89941 gcatgacggg cggtccgcac aatgcctggg ggcagatcaa ccccaagatt tacggaccaa
    90001 aaaacagtgt gtttgccgcg atcgatttcg ctcccgcaac agaccacggc ggatgcgatc
    90061 ccacccccac ctgggtcact gcggcggcgc cgggcctcgt cgtgcgttca agcaatggag
    90121 ccgtgatggt tgacctcgat ggcgatgggt acgagcagac cggttggaac ctgctttaca
    90181 tgcatgttgg ggcaaaggac cgcgttgcag tgggcacctg gctcgaagtg aacgaccgca
    90241 tcgggcatgc ctcttgtgag ggcggcgtat ccacaggcac ccacctgcac tttgcaagaa
    90301 aatacaacgg cgaatgggtt accgccgatg gcccgattcc gttcatcatg agcggatggc
    90361 gcgttgtggc aggcgaaaaa gcctacgaag gcaaacttgt ccgcggcgaa gaagtggtca
    90421 tcgccgaccc ggtcggacaa aaatggtcga acatcttccg aaaagagaat gaataatctt
    90481 tcaagaataa aacaaaaact gtccagcgcg ttcctggttt tcgcattttt actttcagcc
    90541 tgcaatctcg aagcctctgt agaaccccag gttccaacac ccacatctga catcgcaccc
    90601 tacagctttg cgccgacagc tctccccacc cgcccaacgt acaagcccgg tgaactggtg
    90661 gattacattg cccagaacgg ggatacgctg cccgctctgg ctgcacgctt caacaccacg
    90721 gtggaagaga tctttcaagc caacccgttc atcccccgcg aagccaccac catgccgccg
    90781 ggcatgccca tgaaaattcc catttatttt ctggcgttgt ggtccagccc gtttcagatc
    90841 atcccagacc atgccttcgt agatggcccc accgtcatcg ggttcaacac atccgctttt
    90901 gtggcatctc aaccgggctg gctcaaagat taccgcgtgt atgcgggcgg tgactggcgc
    90961 agcggcgccg aaatggttga ttacgtagcc accaattaca gtgtcaatcc gcgtcttttg
    91021 ctggccattc tagaatatca gggcggcggt ctcactgccg ccgagcctcc tgtaaaaaag
    91081 aatttactcg gcttcacgcg ccgctattgg gaaaacccat acctgcagct agtcattgca
    91141 gccaacacgc taaacaatgg atattacggc tggcgtaccg gcgaaatgat cgagttcgaa
    91201 gatacgaatg aaatcatcat ccgccccgac ccgtggcaaa atgccgcctc tgtctcgatc
    91261 caatattatt tttcaaggat atatgacgga caaaaatatg cggtcgccac tggacctcag
    91321 ggcctgttcc tgacatacga gaaatttttc ggcaacccgt ggcaggactc caccatcatc
    91381 atcccaggca gtctagccca gccagaattt caactcccct tccccagtga ccagacctgg
    91441 tattacacgg gcggtccgca tacgggctgg ggaacaggag agccgctggc aggcgtggat
    91501 tttgccccac cctccgataa atcgggctgc tttgtcgcag atgataaaaa ctttgccgtt
    91561 gccatggcag atggcctcgt cgtccgctcc ggcgtggaag gggtgctcct tgacctcgac
    91621 aaggatggca atgagcgtac aggctgggtc attttctacc tgcatcttgc cacaaaagat
    91681 cgtgctccgc tcggcgctga attaaaaaca ggagataaga tcggctatcc ctcatgtgaa
    91741 ggcgggcgcg ccacggggtc acacgtccac attgcccgca aatacaacgg cgaatggatc
    91801 atggccgatg gcatcctgcc attcaacttg aacggttgga tcactcacaa tggcacccgc
    91861 gaatatttgg gaaccctcac caaaggctcg atcatcgtca ccgcctgcga atgcggcgat
    91921 gccttcacag ccatcagcgg cagttttcca taaatagata agggaaattc aataaaaaat
    91981 tccacccaca aaccatacgg ctcacctgca aatttgacat tcacaggtga gccgtattca
    92041 tttttccaat ttaatcaaac cgaatcgcgc ttcgaaaaat actcgccttc ttttatggtt
    92101 tcgccgccat ctcctaaaat tttatatacc atacgaatag ggaacgcctt ggaaagcatg
    92161 gcttgggcag ggcaatactt ctcggctgaa agctcaatgg cgcgtaacac agccgcctca
    92221 tccaccccat tgcctgtgac cacgtattcg atcaccgctt cggtaaagac tttgggatgt
    92281 tcgtttgccc tttctgtatg aatctgaact ttaaaatcca caacaggctg ctgctttttt
    92341 tgcaaaatgg aaatcacatc catggcggta cagccagcca accccaatgc aatgaattcc
    92401 atcggacgag cgccgttgtc atcaccacca accgacgaat ccgagtccat tttttgcaca
    92461 aacccagaat cgcccgcccc ttcaaaagac attttcccct tccaattcaa caatacatcc
    92521 atttttcact ccgcgatcaa atcaggtaca tatttttcaa gatttcgttt tgagatgccg
    92581 ccgatccaca tcaagcggac ggtaccggaa cggtcaatta cataagaact tggcaggttc
    92641 atggtattaa attcacgctg ggcgagataa tccagatcca gccagacggg aaaggtgagg
    92701 ccgaactctt caacaaatgg ctgcacaacc gcggcagtct cttcctgatc cacaccgaga
    92761 aggacaaaac catcctcctt gtatttttcg tagaaagttt gcagggtggg catttcctcc
    92821 ctgcaaggcg ggcaccaggt agcccacaag ttgacaagga caacacttcc caaataatca
    92881 tcaagcgaga cgcttttgcc agaaaggtct acaagatcca atttcggggc gggataatcc
    92941 actttaacgg gaaccgctga aaagtcctga gaagaaacgt cttgttgact taacagcatg
    93001 tagaacatga tgccgatcgc gatcagtcca gcgccgatca gcatggcagc aaagggccgc
    93061 gcggtgggcg gctggggtga ttcgataaga ggggagtcag tcatgttttc acatccaata
    93121 agatttgctc acacggagca aaaagggatt catcaggtac atccgccgcc ttcaccatta
    93181 acataaccac aggcagggca ttccaattgc aacaagccat tgtaatcaag cctgccgagg
    93241 tgacattgtg ggcaaagcgc atctttacgt aacggttcgg tttcaagctt tgggggtgca
    93301 tcaagcccaa gcaggaggcg ttcaagcgca gagagtttac ttatttctgt catcactaca
    93361 ttgaagaggc gaagattcgg aaaattactt atttcacaaa gtataaccca gggacaccag
    93421 cattttttga aaaaaggaaa aatgtcatca agtttatgat atttgatact ggattgttga
    93481 ttggacattg attaacattc tataaatgct tgaactcctg ccgaatattc ccttgtttga
    93541 aaatctcgac acagcccaaa ttgccctgct caagccgctg ttcgaacaat ttaattgtgc
    93601 agcaaacacc gtggtatttg aacagggtga acctgccagt tatttgtatc tgttactcaa
    93661 aggcgaaatg ctcattcgct ttaagcctta tgacgccccc ccgatcacat tgacacgcct
    93721 gcgcgatgga gatgtcttcg gctggtcggc agtgctgggc agcacccatt acacttcaag
    93781 ccttgtcagt gaaacacaga tcgaatccat tcgcatccgc ggctcacatt tgctgatgct
    93841 ggtgcgtgac catcctgaaa ctggaaagat catcatggat cgtctggcaa atgtagtatc
    93901 atcacgttgg aagaacgcgc gtacccaggt ccaggccctt ttgaattcaa ccaatcaaaa
    93961 tataaaggag taaaagccat ggtcgcaaca cagatccaga cacaggaaat gcaattgcgc
    94021 ggtttgcttg aaaaactcag cgcctacgtg gaacaattcc acggcggcac tgtagagttc
    94081 gtctccttcg acggcaaggc tttgcgggtt catttgggag gtgcatgttt gaattgtccc
    94141 ctgctgccct ccaccctgca cggctgggtg gctgggaccg tccatcagtt cttccctgac
    94201 atcgaggtca ttgaagaaaa gtaattcact caagcggtta ttctgggcgc aatttcagat
    94261 tccatcgcaa catagaatgt gaatctaaac atgaaacatc ccgaatataa tctttcaatt
    94321 cgttagccgc ggatttacac gaaaaaagcc gatccatttt attgggtcgg cttttttata
    94381 tctcgtccgt atcctgcaat tcatttgacc tctcgtgtaa tgtctttgaa aggccatttt
    94441 gaaccacgga gccacggagt tttcaattgt tttctctgtg tctcagtgtc tccgtggtta
    94501 gactttcaga ctttcacata aattcgcggc tgattcccta agtttggagt gaatcatgtt
    94561 ttgttcaact gttttactca ccttaaacgt tgcgattcga gaaatagcgg aacagtaaaa
    94621 atccgcctgc tgcgatcatg accactgccc aaaggtagtt ggccagttcc aagagtgaaa
    94681 tgatccccaa cacgcccatc acacccaaac cgagtgcggg ccagtatgcc cagcgcatcc
    94741 ccgtcagcat ggctaccaaa aggaaggtaa tggcaagccc gaagaagaaa atgccagccg
    94801 tttgaaattc gccaaagcgt tgcgccgcaa cggtgaccgt tgccagtgtg accagcaccc
    94861 ctgcagggat gagcgcccac cagcgcgaat tgcgttcggt gaagaagacg aataagaacg
    94921 caaggccgat gccgcctaaa aagatcgccc cgcccaattc ttcatagcgc tgcgcggcaa
    94981 cgatcatcac agccagcgca gacaagacac aggcgggaat gatcgcccac catctttctt
    95041 cacgggcaga aaggaacaca taccaaaacc ccagaccgat accgccaaga aagagcgccc
    95101 cgccaatttc atcaagcgat ttgggcaaca ggaccaaagc accaaggccc agcaatgtaa
    95161 agcctggaat ggcagcccac caatgcccgc caaacaatag agtgagaaag atcagaccaa
    95221 tggaaaggaa tagtccaccc caaaacaatc ttgaggcgtt ctcaaggatg cccattgtct
    95281 gtgcgagcgc caaaccgccg cccaccagaa gcagaaaacc gaacacgata tttgggtcaa
    95341 accgtttcat tacacacctc actaactgat tttcactgaa gccgcacccg catccacgct
    95401 caggtcaacg gcgttggcgg cagtgtcgta atcctgcgat tgataataac caccgctgcg
    95461 cggaaaacga gactcatcca ctttcaaatc aacagcacca actgtggcgc ggattcgggc
    95521 ggagactcct gcggggacga tcacttcaag ggaagccgca ccaagatcga ggtccgcacg
    95581 gaagcggccg cgtgatggca gggtcaattt cgtttcgctg gcaccagttt ccagttttaa
    95641 gtcagtgatg gtcaggtcgc gcaaatcgag cgcggctttg ttcgcaccga gattcatggt
    95701 cagagccatg ggaaggttcg cattcagggc gacatcccaa tcaagctggt tgcctccgcc
    95761 gaaaaatgga aaatccacaa attcttttgc aggcttcatg cgcacatcga ggctatcacc
    95821 attgcgcgtg gacttttgat tcagcccacc tgcaaatgtg ccgcgagcca cctcgctggg
    95881 cgatgcgccg ccatgcagtt tcaactcacc tgcgccgtga ttgatgcgca catgcgcaga
    95941 cgatgctccc tgcagatcaa tggatgcgtt ttcatcttcg atcttgccgc gcatgaacac
    96001 gcccaacagc acccaaccac ccgccagcaa aagggccagc ggccagaaca gacttgaaat
    96061 cgagttgccg ttcggaagcc tgagacccat ctgatcgaac aacaggaaca aaccccataa
    96121 cagcagggct gatccccaaa acaattgact gcgacgcata ttattcctcg cttctatttc
    96181 ggaaggagcg atacaatccg cgccccaaaa cataaatgcc aagcaagatc aacaggatcg
    96241 gagcgccgta atccccaagc aggttccatt ttccgaagaa tgcggaaaag acaaggaaaa
    96301 gcacagcact gacaaccatc atattcaatc cacgtttgag gttgtgggcg gtgttctcac
    96361 caagaatgcc ctgcaacact gtgccaaccc ctacaaaccc agggatgagc gtccacatgt
    96421 atgaccagga atcgtagctg cgggtgactt cttgaaaata aaagatgccg ccaatgccag
    96481 ccacgatcga agcagggacg gaaagccccg gtgagcccag caccaacccc acaatgaaga
    96541 tcgccccgcc gatcaaaacc agattgatgg gccattctgt gtatcgttca aaaaaggtgt
    96601 ggaaggcagg catggtctga tcgagcaaaa accacgcccc gagcaagatc aaaaggatgc
    96661 caagggcaag ttgtgtacgg ccttgtttgt tcatagtgga ttctccttga aacacaaata
    96721 tcaaatctag tttattactg ttcgaataaa aagaaaagtc ttacttcatc tacgcagaaa
    96781 tacggcgaaa gttgtcaggc ggagtgcgcg agggcaaact caaggaccgc ctcaaataaa
    96841 accgacagcc cagcctgtgc ggggcgcaga tcaccagaca tgctccacct gccgccagcc
    96901 agcgaatctg aaatggaaaa ggccgccaag gcgggccgcc cgttcgcttt tgcaaccgca
    96961 tacatggccg ccgcctccat atccactgcc aaaaccccgc gctgctgata ggccagcaca
    97021 tccctgcgca actcacgaaa cggggcatct gttgtccatg tgaggccgtg aaaatacgga
    97081 cgatttccct ttttcagaat agcccccacc ccctcagcca tttcctgaga ggcctccact
    97141 gattcttctt caggaagata gtgccgagtc accccttcac cgcgaattgc ccctgttgaa
    97201 aggatcagac tcccagccga aagattttcc tgcaacccgc ctgccatgcc gatcagagca
    97261 aagcggcgga cacccaacgc aacaaactca tcggttaatc cagcaacaac aggcgcaccg
    97321 ataccaaatc cagtggaaaa ggctattcgc cccttgcttt tcttaagcag gtaaaaaccg
    97381 cccaaaaatc cctttatctg gctgacccaa tatttccgaa ggacataatc agcaaggctt
    97441 ttttgcggca caaagatgac tgtttcaggt ggaggaaaat tctgcaaaga ttttgcagca
    97501 cgacgatatg caagaagttc aacaggcgta agaatggggt cgtcaagact cattggcttg
    97561 aaatctcctg aagtaaaaga acatgaacac aacggcttga aagcattccc caatcagaac
    97621 agaccaaatc gccccacgca aacctgcaat tgaaatcaaa acaagattta cagcagcata
    97681 tcccaaaagt gaaaacagag cagatttgac cacatccgat tcgtagccac gagcaatcaa
    97741 accataggaa atgaatgaag aaatcgtgta tggaagcagg ctccaaccga gcaaagcgag
    97801 caaagacgat gcttgaacga aatcatttcc atacaaaaaa agcatgagtg gattagccaa
    97861 aaaagttaat ccaacgacaa aaagcaaact aactgtcatc aacaagataa aggcttttcg
    97921 aaaactattc caggaacctg ataccccacc tgaaagagcg ggaagcaagg cgcctaaaat
    97981 ggcgtaatgc ccaagtttga gcccatccac cacccgagta atagaagaga ataatcctac
    98041 agcctcatta ttcaaaagcg cagaaaccgc caagacacca agccgctgaa tcaataccag
    98101 caaaatcgtc aacacggcaa aagggaaaat caatttgaag atgggggagg aactttgaat
    98161 tggaaacaag ctgaagttcg gcagggacaa acggcaaacc aaataagaaa cagtcgacaa
    98221 taaagcctgc cctacaaaaa gaaaaataca caaggccaaa acatttgaag aaaaaaaagc
    98281 ggacaggact tgaataaagc cgttcaaaag actgagaagc cagaataaat ccatgcggtt
    98341 caaggcacgt aacaatgcat tgtttacaga gaaaaaagcc agcgggaata gcgcaaggga
    98401 ataaaacaac aaaggcccac ttcgaaaagc aagcatggca aaacagtaaa caaccgagag
    98461 aagcaactgc aaacccagtg cctgcccagc cacttctgta acccgcccaa cacgggcaat
    98521 ttcacggaca aggaatgtgt cagtaccaaa atttgaaaat gtgtttccaa tcaaaataag
    98581 ggaagcgatg aacgaaaact gaccaaaatt attaacacct aatttacgtg caataacggc
    98641 agtaaacaaa accgcaagag cctgcgcggt aagacgcgca agcaaaagcc agaacgaatt
    98701 tcgtcgtatc gacatgacca cagaggtcaa aatctccaga tcaaaaatca gcggcttgca
    98761 tcacttacag cggcaaggga cacgattgta aaatcacccc agtatttaaa cggaaatttt
    98821 gatgccagag catcgtccag tttactaaga gtttcaaaca agcgagggcg ttttattgca
    98881 aaatctttgt ttgttgctgg cggggtaatc actgaaagtg cctcgataga ctcaagacgg
    98941 tattcgctac cccacgcatc agccacttct ttgggtgtgt aataaaaaac ggggtaattg
    99001 agaccttcct tgaacactgt ggattttctc ttaaagcgac gtgtggcaag tttgaatttt
    99061 ccgcgtaaaa gcatggcgga ttcccataaa caaaatgggg gcataagtgc ccaaaccacg
    99121 cgcccatcct gattcatcaa tggacgaacg ctatctgcca caagtctcat atctgaaaca
    99181 caattcaagc ccccgaaatt ggagaaggtc aaatcgaagc ggccaatgct ggcctccttc
    99241 agatcggtgt aggacaactg ttcaaatgac aagcgatctt gcagattaag gtcttttgct
    99301 ttttgttttg cacgttcaag catttcacca gaaatatcaa caccaaacac tgaatagcca
    99361 ttttgcgcaa accacacggc atctgtgcca gtgccacagg caagatcgag aattcgtgaa
    99421 cctttcggca ggcgggcagt gacaaattga tacactcgct gacgcatacg catcaggttg
    99481 ggattgtttt caataaattc gtcatattta tcagcattgc gatcgtacgc ttccgcaaca
    99541 gtttgaaatg cagggttatt caggggcata aaatctccta ggacaaaact caaaaaacag
    99601 acaatttgac aggtttaaca gataaaaact ttctcaacat gtattcgctc ttcgccacgc
    99661 ccaacagata atacgg
//
LOCUS       DAOQLC010000003        86085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_40_length_86085_cov_23.457131, whole genome shotgun sequence.
ACCESSION   DAOQLC010000003 DAOQLC010000000
VERSION     DAOQLC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 86085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40_length_86085_cov_23.457131"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PL000_01125"
     CDS             complement(<1..242)
                     /locus_tag="PL000_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC87524.1"
                     /translation="MTQTVDTLFINAIVLTMDRNLNQYDPGAVAVKGDSIIAVGSEAE
                     IKSEYTASEIVDCKGRVLMPGLVNAHTHVPMTLMRGL"
     gene            complement(307..1299)
                     /locus_tag="PL000_01130"
     CDS             complement(307..1299)
                     /locus_tag="PL000_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNC87525.1"
                     /translation="MSAQRPQRKESIWVTLAWTLNLIGLGGIAVVAIGYVVMTTSFAS
                     AQEAQPISTRALPNTFTPDPNSSLFLPTVTPNPLATRITFQTPTPFVLISKNGNGIRP
                     QIIGYSVAGRPIEVYTFGNGESQRMIVAGIHGGYEWNTIALADELITHVYEHPEIIPS
                     DVTLYILRNLNPDGDARDHGYDGRVNDHGVDLNRNFPTNWAAEWDRDGCWDYRPTTGG
                     TGPGSEPETRALMGFLQSHRVEALISYHSAALGVFPGGEPWDEESTDFARALAEVTDY
                     SFPPLDLGCIFTGTLADYAVELGAVSVDMELATHTRTDFDENLAALGILLNWGR"
     gene            complement(1299..2426)
                     /locus_tag="PL000_01135"
     CDS             complement(1299..2426)
                     /locus_tag="PL000_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNC87526.1"
                     /translation="MTSAEIITIGTEILLGEIVDTNTRYIARTLRSMGVDLYRTITIG
                     DNIDRIASAIHNSMERADIVITTGGLGPTVDDPTREAVAKAVGVETEFREDLWEQVVE
                     TIARYGRKPSENQKRQAYVPKGALGIRNPVGTAPCFIVETERNAVISLPGVPSEMEQI
                     LHESMIPYLQKRFNLNEIIKVRVLHCAGLGEGMIDEKIADLETLSNPTVGLAAHTGVV
                     DVRIAAKARNENEANEMIAVIEAQVRERLGNVVFGADEDKLEEVTLDLVAKRGWALTA
                     IESGLDGILARKIPHTASFSNLEPAQLLEALRAARAESKAEIALGVSVYSADRSAEMA
                     MITPRGEKTHRITYGGPPRSLPKWAMNLALNWLRMTLEEFN"
     gene            complement(2490..3647)
                     /locus_tag="PL000_01140"
     CDS             complement(2490..3647)
                     /locus_tag="PL000_01140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC87527.1"
                     /translation="MDTLPTSADIVIIGGGVMGASAAYHLAHRGLKNIVLIEKEKYFG
                     TGATGRCAGGVRYQFSTEINVKLSIESLPMIERFKEEIGQEVSYRPCGYLLVATNEKD
                     LAEFKHNVALQNRLGIPTQLLSGDEVRARLPLMKFNDAIAGTFNQKDGTVDPNGVVMG
                     YINAAQKMGVKAISDVEVTGVTVSGGKVDGVQTSRGGIKTRMILNVAGPWSGLIGAMA
                     GVTIPIVPLRRQMFTTNPLEEIPADFPFVIDFAKSLYFHREGEGLLVGMSNQNEKPGF
                     DQNVDDDFELVNLEAAIERLPLMEKASRASHWAGLYEVTPDAHPIYGKTDVDGFFLCS
                     GFSGHGFMHGPVSGKLMSEFILDGKFSTLDVSMLDLKRFEEGRLIQEYNVV"
     gene            complement(3720..4427)
                     /locus_tag="PL000_01145"
     CDS             complement(3720..4427)
                     /locus_tag="PL000_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87528.1"
                     /translation="MLELKDVHTYYGNIHALKGISLTVNDGEIVTLIGSNGAGKSTTL
                     RTIQGLNKPRSGSVLLDGVELNKLPAHEIATLGVAQSPEGRMIFPRMTVLENLEMGAY
                     ARKDLASYKEDLEHVLTLFPRLRERISQKGGTLSGGEQQMLAMGRALMAKPKILLLDE
                     PSMGLAPLLVELIFDIIKKLNDEGTTILLVEQNALMALSIADRGYVLQTGEIVLADDA
                     KKLMKNEEVQKTYLGVE"
     gene            complement(4444..5217)
                     /locus_tag="PL000_01150"
     CDS             complement(4444..5217)
                     /locus_tag="PL000_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87529.1"
                     /translation="MLFEAKAITMTFGGLTAVNKVDFNLEKGMIASVIGPNGAGKTTF
                     FNTITRVYTPTSGEMKFDGKDLSGLQPFDITTLGIARTFQNIRLFANMSALENVLVGQ
                     HCRLTSNLIGQVLRTPATMKEEADARARALDLLEFVGLRRSDANVTAKNLSYGLQRRL
                     EIARALASQPKLLLLDEPTAGMNPNETIDLTNFIQRLRKELDLTILLIEHHMRVVMAI
                     SDRVSVLDYGEKIAEGSPSEVQKNERVIEAYLGKGTATK"
     gene            complement(5229..6758)
                     /locus_tag="PL000_01155"
     CDS             complement(5229..6758)
                     /locus_tag="PL000_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87530.1"
                     /translation="MKLIRPGITFGIIGGLLTFLFGGWSVAVLGVLMGVGLGLGLGNN
                     FERKDPLKIALAVLPSALVAAVLLVGLSLYQNNFVMEAIGKKPAKADIAMNANLIGFF
                     GALIFTTLLASLRGLPEKQEQYAKMILLALLVIVFPFVDKATALRWTAQIIFALIFVI
                     LGLGLNIVVGYAGLLDLGYAAFFAIGAYTTGILSSPVHNIQWNFWLVIWIAAAMASVW
                     GIILGAPTLPLRGDYLAIVTLGFGEIVPVLFKNLIDVTIKEPLTCWILPMFGANTTCI
                     VFTEHFDATLGEKGISPIGRPSLPIIGEFESSNPVTWYFLIIAIILLSIFIIRRLRDS
                     RLGRAWMAIREDELAAAQMGINPVRTKLAAFAMGATFSGFAGAFYAAYIRGIFPSVFD
                     FSASIIILCVVILGGIGNINGVIVGGLVLMTADRLFLPALKDFLSSLLTHTVLPSISD
                     PVVQLAVKDNANPILYRFLLFGLTLVIMMAVRPEGLIPNAQIRAELHKEEDEASAEKK
                     G"
     gene            complement(6770..7882)
                     /locus_tag="PL000_01160"
     CDS             complement(6770..7882)
                     /locus_tag="PL000_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNC87531.1"
                     /translation="MENLKKINWRRLGTIAIVLYIVFLIGPIAGLDVPAIVQAIKANP
                     LQLVQFLIIGIANGAIIAIIALGYTMVYGIIELINFAHGDVYMIGVFTSLTVLSVTGT
                     LDSKDPAKIILGLILALVVAVVVCATLNATIERLAYKRLRNAPRLAPLISAIGMSFIV
                     ENIGLMWAAQAWLVPSFGTTGGASQKVFPDMLGTLFTSLGIDVNLLASSGLALRFTIK
                     DLLVIVLAVGLMTGLYLFVQRTRLGKAMRATAQNRDAAKMLGIDIDWVITATFLIGGG
                     LAGAAGLMAGLYNNTAWWFMGFRAGLQSFTAAVLGGIGNITGAMLGGFLIGLLASFSD
                     GYLNARWTNAWVFATLVLILLFRPTGLLGENTGQKA"
     gene            complement(7973..9304)
                     /locus_tag="PL000_01165"
     CDS             complement(7973..9304)
                     /locus_tag="PL000_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC87532.1"
                     /translation="MNKRLLALLSVLVLASMMLAACGGGAPAATEAPAEEPAATEAPA
                     EEPAATEAPAAEAVTLKVVSSLPMTGSSLTQTQTIVNAMQLRLEQANYSACGGNVTLE
                     YEAWDDASAALGKWDPAVETENANKAAADPAIIAYLGTFNSGAAKLSIPILNAAGPLV
                     MISPANTNPGLTKADKDLPGVTDSYYPTGTRSYARVVAADDIQGKVAANFVKSLGATT
                     VYILDDQELYGKGIADVFEATANEIGLTVVGHEGIDPKAADYKALMTKISTSNNGGAP
                     DAIYVGMVVDNNASQLLKDKVAIMGDNETVKFVGPDGIQTQAFIDGAGADVAAGVYAS
                     VAGVPFEELPEAGKQFKADYEAKFGPLTEPYAVYGYETMNVALRAIEDVCAAGGDPSD
                     RAAINAAVFAIQNFDGALGTWSFDANGDTSLTDMTFYQVKDGAYAPVGTFK"
     gene            complement(9554..10036)
                     /locus_tag="PL000_01170"
     CDS             complement(9554..10036)
                     /locus_tag="PL000_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC87533.1"
                     /translation="MVNFLKQGDIFYQFTPTQLELVANLCQEVVFNAGDLVFQENSGS
                     KELYVIAQGEVDILINRGATGESDKKETAVARLRRGQSFGEVALVDEGLRSASARAAQ
                     KDTRLLVIQRDKLIMLCETYPQLGYRLMYNLAADLAMKIRNTDLRIREQLLYKPHDKK
                     "
     gene            complement(10118..11527)
                     /locus_tag="PL000_01175"
     CDS             complement(10118..11527)
                     /locus_tag="PL000_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC87534.1"
                     /translation="MSKNIILLILILSLFFPFQPAQAQDTGGPIYIVQPGDSLSSIAE
                     RFSVSLADLMAVNGIANANQLNAGDALIIPGLSGITGTLNTEVVNFGDSYRSLIRRTQ
                     VSETLFKKLNHVVSPSEFYVGVSMIVPVQSGSQGLNKRISPTKGQSLLELAVQQNTDV
                     WTLSHNNHLAGSWDSMPGDTLFTVGEDNAEQTTSGLPAAFISAEIRDLPIKQGGTGVI
                     KVKTIPNVTLSGLLVDHALHFFPMEDGTQVALQGVHALLPPGVYPLRLEATLPDGSKQ
                     SFEQFILIISGNYPDDPLLYVDPATIDPASTEPELQQLIQLTLPSNPIKYWSGDFISP
                     AIAYAESTYFTSRYGNRRTYIGQGTELQITGFHTGLDFGGGDGLPITAPAAGQVVFAG
                     PLTVRGNATIIDHGWGIYSGFWHQSQINVQVGQVVQQNEVIGLVGGTGRVTGAHLHWE
                     LWVNGIQVDPLDWLILTYP"
     gene            complement(11564..11938)
                     /locus_tag="PL000_01180"
     CDS             complement(11564..11938)
                     /locus_tag="PL000_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNC87535.1"
                     /translation="MPRPIFVHNLNRMIEQPARVGYCDSFSCRLRGLMFRKRLELNEG
                     LLLVEKRDSRLDTSIHMFFVPFDLAVFWINSEMKVVDKIIAKSWHPAYFPKADARYTL
                     EIHPQRFGDYEIGDQVEFKNLQ"
     gene            complement(11996..12391)
                     /locus_tag="PL000_01185"
     CDS             complement(11996..12391)
                     /locus_tag="PL000_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87536.1"
                     /translation="MNNTSIKHIVCIEDEPEMIELIRLILGRRGFDVHGAPGGKEGLK
                     MVRDLHPDLVLLDLMMPEMDGWEVYQQLKADSSTREIPVIVVTARAQNIDKVLGLHIA
                     KVDDYIAKPFGPQELLDSVEKVVGQKLSS"
     gene            12445..13218
                     /locus_tag="PL000_01190"
     CDS             12445..13218
                     /locus_tag="PL000_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC87537.1"
                     /translation="MKIITWNVNGIRAALGKNALDWAFAQSPDFLCLQEVKARQEQLD
                     EEQLAKLKLSFVWNAAQRPGYSGVTTFFKNQPDEVVTGMGREVFDVEGRVIQTRWAGC
                     RLFNIYFPNGTRGHDRVEYKLDFYAHLLELCDALHEKGENLIITGDFNTAHMPIDLKN
                     PKQNEKTSGFLPEEREWVQKFLDHGFVDAYRKLYPEKIEYTWWTYRLDARKRGIGWRL
                     DYFLVSEKLMPFVKDVIVHDKVMGSDHCPVELVLEGEGF"
     gene            complement(13215..14135)
                     /locus_tag="PL000_01195"
     CDS             complement(13215..14135)
                     /locus_tag="PL000_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC87538.1"
                     /translation="MAHSSPNDRLFLTKFAWLSIGAAIFTITLKTIAYFLTGSVGLLS
                     DALESIVNLVGAVMALAMLTIAARPADEDHAYGHTKAEYFSSGVEGALILIAAASIII
                     AAIPRLINPQPLEQVGLGLGVSIAASLANLLVALVLLQASKKHNSITLEANAHHLMTD
                     VWTSVGVLAGVGLVVLTGWERLDPIVAFVVAGNIIWSGFRIVRMSALGLMDTALEPDE
                     QKLVKNVLSSYTKNNVEYHALRTRQSGARRFVSVHILVPGKWTVQRGHRLLESIESDI
                     RKALPNVTVFTHLESLNDPASWDDTNLDRN"
     gene            14393..16255
                     /gene="ftsH"
                     /locus_tag="PL000_01200"
     CDS             14393..16255
                     /gene="ftsH"
                     /locus_tag="PL000_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNC87539.1"
                     /translation="MNSRSQSFFVYFLLIVAIGAMFYMGISENLNTEEPLTINEVALA
                     VQKGEVARIVIGSDDTVRVIFNNGVEEGVESRKEPDATFVDQLRSLGVTTEQLAPENV
                     VIEVSAPSVWAGVLSGAIYILPVILMAGVLWFIFRQAQGSNNAAMSFGKSRARMFSGE
                     HPTVTFADVAGAEESKQELEEIVEFLKEPQKFIQLGARIPKGVLLVGPPGTGKTLLAK
                     AVSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIIFIDEIDAVGRQRG
                     AGLGGSHDEREQTLNQMLVEMDGFGTDTNVIIIAATNRPDILDPALLRPGRFDRRVTL
                     DRPDVKGREAILKVHVKGKPLDPKADLASVARGTPGFVGADLENLVNEGAILAARRNK
                     KSIGQSELEEAIERVVMGPERKSRLISDEEKRIIAYHEAGHAIVANAIAEADPVQKVT
                     IVGRGQAGGVTWFRPDEDRILMSRKKMLATLAMALGGRAAEELIFDDITSGASNDIEQ
                     VTRMARAMVTRLGMSAEMGTMTYGQKEEMIFLGREISEQRDYSEAVAEQIDREVRKIV
                     EESYKIARNTVKKYRKQLDLVAKQLLEVESITREDFEKIFPAPLGKKSGTPQAV"
     gene            complement(16340..18040)
                     /locus_tag="PL000_01205"
     CDS             complement(16340..18040)
                     /locus_tag="PL000_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNC87540.1"
                     /translation="MNNRPWLANYDKGVPQTIDYPKAPLFHFLEEAARQYPDRACTIF
                     KGAVISYREMNALTDHMAAALVEMGVKKGDRVGIFMPNTPQFVIAYYGILKAGGAVVA
                     VNPTYPVDEIITPVNDANIEIMFTLSRFYDKLKTARERSKLKKIIVTNIKETLPPITR
                     LLFTLVKEKKEGDRVDTLASGDVWMKDLLAKHANSPKPNVEVTPDDTALFQYSGGTTG
                     VPKGAVAMHRNVVANTLQIKSWMPALEPGKEVVLMGIPLFHVYGMVAGMNFAMANGAS
                     MVMVPNARDLKDVLENISKYKATIFPGVPLLYNGINNHPDVKAGKYNLSSIKACISGS
                     APLMRETKDEFERLTGGKVFEGYGLSEAPTATHCNPLNGVNKTGSIGMPLPDVDIKLI
                     SLDDGETELPQGEIGEIIVNGPQVMKGYHNMPTETANSLRQMKDGKTWLFTGDIARMD
                     EDGYFYIVDRKKELIKPGGFQVWPREVEEAIMDHPKVLEVGVGGIPDPQRGETVKAWV
                     VVKPGETLTEAELKAFCKEHLAPYKVPTHYEFRTELPKTTVGKILRRELVRQHKEGGK
                     "
     gene            complement(18081..19016)
                     /gene="miaA"
                     /locus_tag="PL000_01210"
     CDS             complement(18081..19016)
                     /gene="miaA"
                     /locus_tag="PL000_01210"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNC87541.1"
                     /translation="MSLNKPPLILIVGPTAVGKTELAIQLAESLNGEIVSADSRLFYR
                     GMNIGTAKPSQEEMNRIPHHLIDIVEPDETLSLAVFQQEAKRIITEIHTRGRLPFLVG
                     GTGQYVRAVTQGWQPPEVVENPKMRDELEKTKEERGAEWLHAKLAYLDPESALKIDAR
                     NVRRTIRALEVILTTGRKFSEQRGQGEAPYRLITIGLTRPREELYQRVDERIEAMFSA
                     GFVDEVQSLLERGYAPSLPSMSAIGYRECVSVVQGQLTTEQAKVEMKRATRVFVRRQA
                     NWFKESDPEIAWFRVKEGVVMEVEKHIHNRLTFDS"
     gene            19167..20021
                     /locus_tag="PL000_01215"
     CDS             19167..20021
                     /locus_tag="PL000_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87542.1"
                     /translation="MINWQELTIDMFVEQLRTVYQRTYGDINSDFGRIVAWCGRLALE
                     NIANSDALYHDIDHTIMVALAGQAIVEGKHLREGGVTPRDWMHFMIAVLCHDIGYVKG
                     VCNNDTKDQFTTGVGNKMVFISPEGTDVALTPYHVDRSKQFVIERFGTGLLQDMTKFL
                     DAELIASYIEMTRFPSPPGEMYKDTKGLGGLVRAADFIGQLGDPDYLRKSPALFYEFQ
                     ELGVNEKFGYKSPNDLRKNYASFYWNSVSPYIQDALHYLRLTEDGKQWIANMHSHVFD
                     AEHDRGAR"
     gene            complement(20126..21073)
                     /locus_tag="PL000_01220"
     CDS             complement(20126..21073)
                     /locus_tag="PL000_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC87543.1"
                     /translation="MTEISNETRFLHAVEMGLGAWQWGDRIVWGYGQTHTDKEIRESF
                     DASLALGIRFVDTAEIYGSGYSERLLGQFLKETDQPVLVATKFFPMPWRLTKGFIPRA
                     LKGSLERLGLESVDLYQIHWPSPLMTPETMMEGMVECVQRKLTRTVGVSNFGEKRMIR
                     AYTTLARHGIPLASNQVHYSLLSREVEKNGTLARCKELGIRLIAYSPLEKGLLTGKYS
                     SENPPPGVRASQYAELIKKLPPVLKLLQEIGLNHGDKTISQVALNWLICKGALPIPGA
                     KSLQQAESNAGGAGWRMTDEEVAKLDEATDDIREYSKPA"
     gene            21255..21962
                     /gene="rnc"
                     /locus_tag="PL000_01225"
     CDS             21255..21962
                     /gene="rnc"
                     /locus_tag="PL000_01225"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNC87544.1"
                     /translation="MPNLNAREVESASDLSRRLGLPFSNLALLTRALTHRSFVNENPM
                     AVEDNERLEFLGDAVLDFIVGAWVYNRFPEMPEGDLTKVRSALVRNEQLAKFARKLEL
                     GNALRLGRGEAASGGSNRDNLLGSAFEALVGALFLESGLGGVEAFVHPILEDARELIL
                     NEIHDPKSKLQEWAQAQKLGAPRYRVIGTSGPDHLVTFEIAVEIAGAERGRGTGTSKS
                     HAEHAAAQDALNQLNVL"
     gene            22046..25654
                     /gene="smc"
                     /locus_tag="PL000_01230"
     CDS             22046..25654
                     /gene="smc"
                     /locus_tag="PL000_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNC87545.1"
                     /translation="MPLRLKSLELQGYKTFASRTVFEFASGITAIVGPNGSGKSNIAD
                     SLRWVLGEQSYTLLRGKKTEDMIFSGSEHRARAGMAQATITFDNTEQWLPVDFSEVAL
                     SRAAHRDGHNDYLINGQHVRLREMNELLAQAGLSERTYTILGQGLVDASLALKADDRR
                     RLFEEAAGVGLYRARRDEALKRLENTERNLERVLDIMAELEPRLRSLERQAKRAIDFA
                     RAQADMKLILREWYGFHWHRAQRDLTDARETVRGQEVRVRDLRAVHEKAQAEYTSFRE
                     RLSGLRAQLNGWHRQSADLHNQREAISRELAVLEERRRALTNTQASILSDQEHAMDEL
                     HLANDRFGEAEQELLRVQGEYEEAKRQLETCNLQLANRNAERATLEEQAQLLRNQIEA
                     FSQQRAENNARLDELKSRLEAQSQRIEQTKQAITSGESLAAKARSQYESAHKAREQAA
                     LALQEAEEKVISRRNEVERLERERRDALEKRTIEESDHSRLKAQLEVLQQSEQSLAGY
                     AEGARYLLDAARRDPQRLTGVRGALSAALDVPAELETAIAAALGDTLDAVLLDENQLE
                     EALGLLESDEAGRAALLPLVMQENILHHPSDENCLGIASELVNAPEEMRGAVRLALGQ
                     TLIVRNRNTARILVKDLPTHARVVTLRGEVFRGDGLIIAGKTASSSTLGRSRQKREFE
                     SALSGLVVRLAESNDLVERLSNQLAGAQRELAQADTDARESRVRLGEIQETEQQAGLE
                     SESTSRQLEWQKKQLEQLEAEAWESASTQQKITESQAEVEQRSTETLSQLKNINARLN
                     EMAVDELQDQVSYWGTRVAVAEQSVGSAAAKRDERTNEITRLDSRRVELISRLQEAEG
                     LLNNLDNEKARLRENESGLHGQIDEMRVLIEPAEKDLEMAEQEEARLQEAEAAAQRAF
                     ANAERSYGQVQLEQVRKQEALDNLHQKITDDFGLVMFDYAADVSGPVPLPLDGMVEQL
                     PVVTELAPEIEEQLTHHRAQLRRMGPINPDAKTEYDQESERYTFMKAQVEDLRKAEAD
                     LKQVVAELDEITKNEFVRTFDAVDKQFRETFVRLFGGGSARLALTDPENLVDTGIEIE
                     ARLPGRREQGLALLSGGERSLTAIALVFALLKVSPTPVCVMDEVDAMLDEANVGRFRD
                     LLIDLSKDTQFIVITHNRNTVQAADVIYGVTMGRDSASQIISLRLDQVTDDMLKRG"
     gene            complement(25820..27250)
                     /locus_tag="PL000_01235"
     CDS             complement(25820..27250)
                     /locus_tag="PL000_01235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC87546.1"
                     /translation="MPNENPVLHKKHVARLQREKQQSQIILYTFIGILAAVLLLVLYG
                     YLDINYFQLKRPVAKVGETEILAKQFEARVRLQRQQLLAQYQTYSQYAQFGLDVSSQL
                     QQIQASLDSPTSIGRAVLDQMINEEIIRQEAAKRGITVSEEEISELTEGAFGYFPNGS
                     PTPSVTPTEVIMPEPPAGAFDIVTITPLPSATPENTSEPTGTATSAPAATIDPATITG
                     TPEATATLVPTATFTPTATLTVTPTLGPTNTPEPTATPYTAEGFQTEFNDANERLMEL
                     GFDDNVYKLVFENQLFETKLKDIITADVAHTEEQVWARHILVEDEATALNLIEKIKAG
                     EDFAALAQEFSTDTGSGALGGDLGWFGTGAMVPEFETAAFALENPGDFTTTPVQSQYG
                     FHIIQLIAKQERPLTADQYNAARDKVFQEWLTAAREEYGVEIFDFWQERVPSEPNFST
                     IATEAANAQDTAVAETKSAVDATAAP"
     gene            complement(27512..27799)
                     /gene="rpsR"
                     /locus_tag="PL000_01240"
     CDS             complement(27512..27799)
                     /gene="rpsR"
                     /locus_tag="PL000_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNC87547.1"
                     /translation="MSEERNYSGGGEGGGDRRFFAKPKFCQFCADKMLVIDYKKTDLL
                     RKYITEEGKIRPRRQTGACAKHQRVVAAAVKQARHVAFLPFSGRALDDNRS"
     gene            complement(27817..28239)
                     /locus_tag="PL000_01245"
     CDS             complement(27817..28239)
                     /locus_tag="PL000_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC87548.1"
                     /translation="MSRGLNKVQIIGHLGKDPEMRYTPSGKPVTTFSVAVSRSWNSAD
                     GERHSETEWFNVVAWGNLAEICKQYLVKGQQVFVEGRLQTRRWDDKEGQKHTSVEIVA
                     NEMMMLGDRRDANNNHSQDADLSSADNGSSMAEDEFPF"
     gene            complement(28284..28568)
                     /gene="rpsF"
                     /locus_tag="PL000_01250"
     CDS             complement(28284..28568)
                     /gene="rpsF"
                     /locus_tag="PL000_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNC87549.1"
                     /translation="MRNYELVCVMQPDLDENAFNGVLDKVKGWISDSGGSVDKVDVWG
                     RRKLAYIIKKHREGQFVLLNVSMNPSATSDLERNLRYQETIIRHMLSVVG"
     assembly_gap    28756..28855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28856..29916)
                     /locus_tag="PL000_01255"
     CDS             complement(<28856..29916)
                     /locus_tag="PL000_01255"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNC87550.1"
                     /translation="MSEVIVNLHMHTRYSDGSGTHQDIAKAALAAGVDAVIVTDHNVL
                     VQGFEGYYKEKNKKILMLIGEEVHDQARDPQKNHLLVFGSGRELATFAHDPQLLIDQV
                     QASGGLAFIAHPDDPAAPAFKETDISWEDWSVRNYTGIELWNALSELKTVVPTKLHGA
                     FYAFFPFFVAHQPIPNTILKWDELLATGARVVAIGGSDAHALRMSMGPISRVIFPYEF
                     HFRTVNTHLILPEGLSGDVNNDKKLIYEAMAAGHCFVGYDMPAPTRGFRFTAQGLEKT
                     AIMGDEIPAKGGVTLQAKLPSDAEIRLLKDGVVIQTWKNKSSCTHITTDAGVYRIEVY
                     RRYLGKLRGWIYSNPIYVR"
     gene            30056..30280
                     /gene="secG"
                     /locus_tag="PL000_01260"
     CDS             30056..30280
                     /gene="secG"
                     /locus_tag="PL000_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNC87551.1"
                     /translation="MEKFLDIALIITSVGLILGVILQSKGAGLGGLTGADTGGVFTAR
                     RGIERILFWVTIGLAVIFFGLVFAVILIGG"
     gene            30362..32053
                     /locus_tag="PL000_01265"
     CDS             30362..32053
                     /locus_tag="PL000_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC87552.1"
                     /translation="MKKLRWQILVVAVTVVIVALLLLSQQPISTSFLPEAAPGGIYTE
                     ALVGSMGRLNPMLDWNNPADRDINRLIFSGLIRFDSHGLPQPDLAEAWGTSPDGTLYN
                     FSIRSNAVWHDGQPVTSDDVIFTIEMIKSSGSLFPQDIKDLWAQIEIKRFDDKTFQFK
                     LPEPFAPFLDYVTFGVLPKHLLETVPADQLATAQFNLQPIGTGPYKFERLLTSGGQIV
                     GVALAVNKEYYLPSAFIEQIVFRFYPDSASALDAYQQGDVLGVSQITNDVLQQALMEP
                     TLSVYTSRLPQLGLVFLNLNNPTVAFLQSDKVRHALLLGTNRELIVSRIMQGQAIVAD
                     GPILPGSWAHYDEIEHFEYDPDAAAALLKAEGYVIPASGGDVRAKDGQYLNFTLTHPD
                     DEIHTRIAQAIQSDWALIGVRLDLQPVSYDSLVNDYLTTRNYQAALADLNTARTPDPD
                     PYLFWHQSEATGGQNYSQWDNRTASEFLESARTEADFSSRARLYRNFQVVFTKDMPSL
                     PLYYPVYSYGVDAQVQGVQVAPMYDVSDRLALVAEWYLVTRRALEEQTPVPTAAP"
     gene            32094..33461
                     /locus_tag="PL000_01270"
     CDS             32094..33461
                     /locus_tag="PL000_01270"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNC87553.1"
                     /translation="MSDAKKAIEYAHQNRERFTRELTEFIKIESISTDDAYKAQVLQA
                     AEWAAGHLRSIGIENVKLMPTGGHPIVYGDWIKNPGAPTVLIYGHYDVQPVDPIELWE
                     TSPFEATVKGDYLFGRGTSDMKGQVMATLNAVEAIMKTGDFPVNLKFMLEGEEEIGSM
                     NMGNFLPQHKDLFKADFCLNPDAGAISADEPTITFGLRGLAYFEVHVYGPTRDLHSGL
                     FGGTVHNPAQALAELIAGMHDANGTITLPGFYDSVRPVTDQEREATAKLPTTPESYLQ
                     QTEVPALWGEKEYTPALRTGARPTLEVNGLLSGWTGPGSKTVLPAKAMAKISCRLVPD
                     QDPAEVHKQLVRYMEEHAPKTITWEVKILHDSPWAITDVNNKGVRAMAAAMETVWGRP
                     PLYRREGGSIGVVAMLQNICGVESGLVGGGLPDDNIHSPNERMHLPTWHKNMDAFIHF
                     FFNMK"
     gene            33517..>34387
                     /locus_tag="PL000_01275"
     CDS             33517..>34387
                     /locus_tag="PL000_01275"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNC87554.1"
                     /translation="MEDRIITYGGQAVLEGVLMRGQKAFAIAMRAPDGSITIHTEKLA
                     KVYRSGITKVPFIRGVILLWDALGLGMKALTISANTQTGEDEKLEGPALYLTLALSMT
                     IGIGLFFLLPAGIGGAAEHYLGWSSWLNNLTEGLVRLGLLVGYVWAISFMPDVKRLFM
                     YHGAEHKTINAYEAGAELTPEVVAQYPIEHARCGTAFLLTLVLLSILVFTLLGPLPLL
                     WRLASRILFIPILAGIAIEYIRWTANHLDSPFVQFLIKPNLALQHLTTRTPDLAMLEV
                     AIQSFQSMRKAESE"
     assembly_gap    34388..34487
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34488..35830)
                     /gene="der"
                     /locus_tag="PL000_01280"
     CDS             complement(<34488..35830)
                     /gene="der"
                     /locus_tag="PL000_01280"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNC87555.1"
                     /translation="MKPVVALVGRPNVGKSTLFNRLAGERLAIVDDTPGTTRDRLFGE
                     AEWNGRTFSIVDTGGIDPTHGGKTPLSVGSVEFIEDIRRQAQVAIQEADAVLFINDGE
                     TGVTEPDREVANILRRSQKKLPDGTFWPPIFLVVNKCESQERRDSASEFYEFGLGDPF
                     AISAIHGTSTGDLLDALVSSFKAPENEEEDDSIKIAIVGKPNAGKSSLLNKLTGKDRV
                     IVSPIAGTTRDSIDTKMEYEGLPITLIDTAGIRRRGRIEHGVEQYSVLRAFKSIERAD
                     VALLMIDATTGITAQDTHIAGYILDEWKSCVVLVNKWDAIEKNNETMEEYTIKIRHEL
                     NFMDYVPILFISAKTGQRMEQVLPLALQVQEERLARLTTSTINGIIHKAQDAHPHPTH
                     AGRALKMYYGTQVRSDPPTFLIYVNEPKLMHFTYIRYLENQIRAEHSFLGTPIRIV"
     gene            complement(35846..37093)
                     /locus_tag="PL000_01285"
     CDS             complement(35846..37093)
                     /locus_tag="PL000_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNC87556.1"
                     /translation="MFRWISENYRTFLWSFALAMAVWISAVTSADPDETRTLPSAVPI
                     QVVGQNPNLVLGSELPTEMELTLRAPRSVWDVIESDPQSIRAVLDLSGLSSGEHTSEV
                     QIQIDARPVQIVSASPRTVTFLLESLDTQTFDLNLSLSGEAAIGYQIGEPVLETTEVV
                     VAGAQSQVEKVARARVQVNVSGLREDFDQTLPVEVLDEKGQTVEGVTVSPEAIHISLP
                     VSQQGGYRDVAVKVIVSGRAASGYRLTDISVYPPIVTVFASDPQLVNNLPGIVETQPL
                     SLQNAQENINTRLALNLPEGISIVGEQTVLIQAGISPIESSVTLGSEKVEIIGIDPGL
                     TAQVSPMTVDVILSGPLPLLDTLTRQGVRVTVDLTGLGIGTHQVIPKVEVLISDIVVE
                     SILPNTLEVVISLIGTPVVTPTP"
     gene            complement(37175..38050)
                     /gene="cdaA"
                     /locus_tag="PL000_01290"
     CDS             complement(37175..38050)
                     /gene="cdaA"
                     /locus_tag="PL000_01290"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNC87557.1"
                     /translation="MTEILNNVFFIFQRISWLSVLDIMLVTLIFFGLLYSLRDTQAMA
                     LLRGMILLVVALILLTSLVDLPAFSWFAQNSLPALLLALPVIFAPEIRRTLERLGRAG
                     TIWPASVKSTDVALEQTIRAVVSAAARLSARQHGALIIMQRLDTLDELAQSGVQMYAR
                     VTPELLLQIFYPNTPLHDGGVIIVGSQIVAAACVLPLSASGILNRTPERQMGLRHRAA
                     LGTSETSDAIAVVVSEETGAISIAHAGRMLRRLDPDRLENILTAFFRPNGKDHKPNLL
                     ERLFPGLIPTKKDKP"
     gene            complement(38210..39364)
                     /gene="coaE"
                     /locus_tag="PL000_01295"
     CDS             complement(38210..39364)
                     /gene="coaE"
                     /locus_tag="PL000_01295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC87558.1"
                     /translation="MSNWPGKFVIGLTGNIATGKSVVRKMLEHLGAYTVDADALSHRA
                     YAKGAPGYQQVIDRFGKWLLNKDGEIDRSKLGNLVFNDAEALAQLEGIVHPLVRQATE
                     MLIKRSTQNVVVIEAIKLLEGDLRKVCDSIWVTNATEDIQIERLIRKRGMNRDQARER
                     IHMQSAQSAKVAVANIVITNTGSFDDLWKQVSTAWKEIVPASVAKPADTEQTLSKVPV
                     TSNGTFTVQRGKPKHSPAIAELITRLSKGKRTVTVVDVMEDFGEKAYMLLLLDGKMVG
                     IAGWQVENLVTRTTDIFLEEHVNPQKALEILIKEVERASSELQSEASLIFPLNELAAQ
                     EELWKQLGYEKRTPETLGVQAWQDSASEALTEGGALLFKQLRQDRVLRPI"
     gene            39511..39948
                     /gene="rnpB"
                     /locus_tag="PL000_01300"
     ncRNA           39511..39948
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL000_01300"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            40010..40324
                     /locus_tag="PL000_01305"
     CDS             40010..40324
                     /locus_tag="PL000_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HNC87559.1"
                     /translation="MQKESRLSTIVEQLIHKSMQIVSVQVAVGELVPMSNEQIHAQWQ
                     EGPLAHIVLNIRRVPAEQQCMVCFQKYHPNQKEVACPYCGANGAKVISGEEFFLEAIQ
                     EK"
     gene            40371..41195
                     /locus_tag="PL000_01310"
     CDS             40371..41195
                     /locus_tag="PL000_01310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC87560.1"
                     /translation="MHIKGKVVIVTGASSGIGEATARAFGLEGAKVVLAARRVEKLQA
                     IAQEINALNTGAETLVVPTDLSKLADIQNLIAQTLARFGRIDVLVNNAGIGRVDWLEN
                     LDPVCDIQTQFDVNVMGMIQATRLALPTMIQQRSGNIINMCSIAGLVATPTYSIYAAS
                     KHAVQGFSEALRREVKPWGIHVSLIYPGGVATEFAQRAGIKRKTNAKTPAFMLLTAQQ
                     VGDAIVDLVRRPRRMRIIPWLWAVTVFLNKFLPGIVDHTTINRFTIPERKDELATG"
     gene            41255..41599
                     /locus_tag="PL000_01315"
     CDS             41255..41599
                     /locus_tag="PL000_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC87561.1"
                     /translation="MGFLSNLFGPSVPKLGAMEVSEKMKFGKHPILVDVRQPEEFRQG
                     HIAGAKLIPLNELHKRIKELPQGREIICVCASGSRSSSAAKMLSREGYTVFDLQGGML
                     AWRHAKLPIQKG"
     gene            complement(41643..43367)
                     /gene="recJ"
                     /locus_tag="PL000_01320"
     CDS             complement(41643..43367)
                     /gene="recJ"
                     /locus_tag="PL000_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNC87562.1"
                     /translation="MTPDTSNTKRWVIPTSITPEADSALQAFPSILRQILFNRGYATD
                     AEARAYLNAKPDSNNDPFLMTGMREAIERIQFAIDHHEPIAIYGDYDVDGVTATALLV
                     QALEYLNADVRGYIPNRFEEGYGLNNEAMDKLQADNVKLVITVDCGIRSPNEALHART
                     LGLDLIISDHHHPAEGELPPALAVINPKQHGDIYPDKELAGVGIAYKIAEALFSIQGK
                     PLAPHLDSLLDLVALGTVADLAPLVGENRMLVRRGLRQMKRTLRQGLSSLAAVSAVKL
                     EKVNAGHIGFSLGPRLNAAGRLKEALAAFELLTTKDVFRAGELAQTLDLQNRERQRIT
                     REIQSRAEDMAMNDDPNAYLLFAAHEDFNSGVVGLAASRLTETYYRPSIVAYKGAEET
                     RGSCRSIPEFHITEALDQCADLLVRHGGHAAAAGFTVKNENLPELVSRLKTIAADKLS
                     NEDLRPTVTADAEVSLVDIRPELFENALRYLEPTGYGNPNASFVARNIKVKSARTVGS
                     DAKHLKLILEDAKGYTHDAIGFKLGDWQKKMPPRVDILFNYEVNEYNGRIGYQLNLKD
                     IKATGARD"
     gene            43456..43971
                     /locus_tag="PL000_01325"
     CDS             43456..43971
                     /locus_tag="PL000_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87563.1"
                     /translation="MKALAYDEKTAMVMVYTDSMLVRGEVVAKENVRVSIWLRTQGVP
                     NYIHLHGAQVISFSGGSAKNYTQAEIFIPTVQVCAFHLAPPAHDPLDYDASEINRVMQ
                     PVNIMVGSFMLRGKMRISSQTEFGTSLDVMRASWASVYEVEVSNPFLPQFNMQVPMLL
                     VNPSHVTFGLA"
     gene            44031..44104
                     /locus_tag="PL000_01330"
     tRNA            44031..44104
                     /locus_tag="PL000_01330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44065..44067,aa:Pro,seq:cgg)
     gene            44265..44609
                     /gene="rpsP"
                     /locus_tag="PL000_01335"
     CDS             44265..44609
                     /gene="rpsP"
                     /locus_tag="PL000_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC87564.1"
                     /translation="MVRLRLRRIGLKGQPTYRIVAADKEAPRDGRFLEILGVYNPRTN
                     PATINLKEDRIFHWMKNGALPTESVAQVFKASGTQARFERLKKGESLEALVAEAAEAQ
                     AKRAAPVKTRKD"
     gene            44726..44962
                     /locus_tag="PL000_01340"
     CDS             44726..44962
                     /locus_tag="PL000_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNC87565.1"
                     /translation="MKELIEYIAKSLVDHPEDVQVRESGGGSRVRIELSVSKDDMGRV
                     IGKSGKVANSIRTLLKVAAERSGKQATLDVMEPQ"
     gene            44959..45519
                     /gene="rimM"
                     /locus_tag="PL000_01345"
     CDS             44959..45519
                     /gene="rimM"
                     /locus_tag="PL000_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNC87566.1"
                     /translation="MTAKQKSTKQTESGSPAGEPVYLVIGYLRRSHGIHGEIIMDLHT
                     DFPERIKAGRKIFIGENHQPHTLGGVREHGNGVLVKLRGIETPEEVGRFRNQWVYVKA
                     ADVPPLPEGQYYKYELIGLDVVDDQGNALGKLNEILETGANDVYVVKNESGKEILLPA
                     IPPVILDVDMGQRVMKVHLLDGLVDG"
     gene            45544..46650
                     /gene="dprA"
                     /locus_tag="PL000_01350"
     CDS             45544..46650
                     /gene="dprA"
                     /locus_tag="PL000_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNC87567.1"
                     /translation="MDDRKYWVGFNLIKGIGAVRMQGLVAYFGDLASAWGADPASLAE
                     AGLGAKVIERVLAARQKVDLDQVWNKIQLQGIKILTWQDEQYPSRLKEIDQPPPVLYI
                     RGEYLLDDLFAVAIVGTRRVTPYGRQVTEEISSFLAANGITVVSGLARGVDAVAHQTA
                     LKAGGRTIGVLGSGVDKIYPPEHQKLAEQMMERGAVISDYAPGTPPDASNFPPRNRII
                     SGLSLAVVVIEAGETSGALITAEFAAEQGREIFAVPGSILAPQSKGTNKLIQNGALPL
                     LSANDLMQALDITRIGEHKTARKILPADETEARLLGVLGHEPLHVDEIRMQADLPIEK
                     VSATLALMELKGMVRQVGGMNYVSVREAQSEYKT"
     gene            46709..47800
                     /locus_tag="PL000_01355"
     CDS             46709..47800
                     /locus_tag="PL000_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNC87568.1"
                     /translation="MENTYAVIMAGGGGTRLWPVSRKERPKQLLPLLGQDTLFQSTVA
                     RLKNMFPYERILVVTVEEQAREMRLQAPEIPEENYIIEPAPRGTASVVGLAAAVLHKR
                     DPKAKMAILPSDHFIRNRDLFHYLLNAAFDVAENGYLVTLGITPTQPSTAYGYIQQGE
                     SLDGEYKYPAYRVKRFVEKPDELTAQKLLHTGDHSWNSGMFIWRADVILDEIDKQMPE
                     LGGALKKIAAAWGADQQGAVLDENWQTLKTETVDYGIMEHAERVAVLPAGGLGWSDVG
                     MWSSLFEVLLPDMNGNISTNSSLHLAHETHNTLVYGGNSDRLIVTIGVENMVIVDGGD
                     VVMICKTDHAQKVKDVVGHLKKHNQEKYL"
     gene            48002..50296
                     /gene="topA"
                     /locus_tag="PL000_01360"
     CDS             48002..50296
                     /gene="topA"
                     /locus_tag="PL000_01360"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNC87569.1"
                     /translation="MKCKTKREMKDPVAGFNAKGSPVTTGTCPECGTKLYRMGKTDAH
                     ADMVAPPRPEKVVVRDGKLVIVESPAKAKTVGRFLGKGYTVRASVGHVRDLLKSQLSV
                     DVENNFEPKYRVPNEKKDVVKEIKKLASTAEEIYLATDPDREGESISWHLMEAAQIEP
                     ERTKRVVFHEITAPAVAEAFSHPREINMDLVNAQQARRVLDRLVGYSISPILWEKVRG
                     RLSAGRVQSVALRLIVDREREIDEFKPVEYWSIHAELKHGSAKTSFLSKLVRIDDKEP
                     ELPNEETVRPILVDMETAAYAVTKVKRGERRRKPAGPFTTSTLQQEASRKLGFTAKRT
                     MGLAQGLYEGQDVGEGGTTGLITYMRTDSMNVSALAQNEAREYVSGKYGSDYLPAEAP
                     MYKTKSAGAQEAHEAIRPTSVMRDPEKVKEFLDPAMFKLYRLIWQRFVASQMESAVFD
                     TLQVEVTGKTSAHEYLLRASGSAVKFPGFLVVYEEAKNEDVKTEEDEENVKIPAGIAE
                     GQEQELVRLIPEQHFTQPPPRFSEASLVQTLEENGIGRPSTYAPTISTIQQRGYVERV
                     DKRLIPTDTGLQVNDLMVQYFPEVVDFNFTAHMEEDLDKIADGEMIWTDAIREFYNPF
                     AEDVKKAQAEMPVTKSGPEPIGRACPTCGKELVIRYGRFGKFISCSGFPECRYTEPWL
                     EKIGVSCPKDHGDLVERKTRKGRTFFGCMNYPNCDFTSWKRPIAVPCPKCSGLLVIAN
                     KREAQCTNCSESFLLEEIMPETVE"
     gene            50351..50536
                     /locus_tag="PL000_01365"
     CDS             50351..50536
                     /locus_tag="PL000_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87570.1"
                     /translation="MHFTPVPYLDPGSGSFIIQILIAALLGLGVALRASWGKIKGWFG
                     IKPKAEDEDGESADASN"
     gene            50523..51905
                     /locus_tag="PL000_01370"
     CDS             50523..51905
                     /locus_tag="PL000_01370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC87571.1"
                     /translation="MPATEQLSASFRDPSGFLFSRNGVLYRQINRAYEKDYARLMDSG
                     LYEKLVKAGLLIPHVESDQAPAESDSVFKVIQPERVPFISYPYEWSFSQLKDAALTTL
                     AIHKRAIKAGMALKDASAYNIQFVRGKATLIDTLSFEIYKEGEPWVAYRQFCQHFLAP
                     LALMAYCDVRLSQLLRVYIDGVPLDLASKLLPVKTKFNFGLLTHIHVHAGAQKRYSDK
                     AVETRKGTTGVSQQAMIGLIESLEAAVKKMTWKPAGTEWGDYYENTNYTDGAFEHKRQ
                     LVREWTLEKKPTLVWDLGGNTGVFSREAVSSGAFAVSFDIDPAAVEQNYRTVRSKKEQ
                     NILPLVLDLTNPSPSLGWDHMERDSFGARGPVDMALALAVIHHLAISNNVPLPQLASF
                     FAARSKWLVIEFVPKSDSQVRRLLTSREDIFPNYTREGFESAFSNRFTIHKAEAVRDS
                     ERILYLMEAR"
     gene            51978..52973
                     /locus_tag="PL000_01375"
     CDS             51978..52973
                     /locus_tag="PL000_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87572.1"
                     /translation="MQFVVAFLKKLPAVPIALFVGGLVLGLVFGWVIWPTEYTNTTPD
                     SLRADLQEDYLRMAIDSYRVNLDPDLAIQRWFNLGIGADQAFKNIQANPGKLDPAVIQ
                     AYGETVTKILATGVQPPVNTGGSATLLRSIFLWGGLLVLLGIGGYGGWYMYRLLGKRG
                     SGEVTPTMQAAELNRSAEKTNFEQLGLAPPITQTMTTYVLGDDLYDESFSIDTQAGEF
                     MGEYGVGVSEAIGVGEPKKVTALEIWLFDKNDIKTATKVLMSQHAFNDPTIRARLEPK
                     GELILVEPQAQVLLETATLQLLATVVDLEYGHGPQPADSYFERITLELAIWPRQG"
     gene            complement(53021..53485)
                     /gene="greA"
                     /locus_tag="PL000_01380"
     CDS             complement(53021..53485)
                     /gene="greA"
                     /locus_tag="PL000_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC87573.1"
                     /translation="MSSINYLTPEGEAKLTAELQELKGPRREELSQRLRSAIQMGDLS
                     ENADYHKAKEDQGFLEGRIQELEAILRSAVIIDNSQTKDVVSVGSRVTIQEADYDPET
                     YHLVGATEADPRKGKISIESPIGKALMEKKVGQVVEAETPGGKIKFKIISIE"
     gene            complement(53567..54715)
                     /locus_tag="PL000_01385"
     CDS             complement(53567..54715)
                     /locus_tag="PL000_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNC87574.1"
                     /translation="MFTRQQLEEIEDKSLASYGMRSKDSKGRAYLDSEPDYRTSFQRD
                     RDRILHTTAFRRLEYKTQVFINFEGDYFRTRLTHTLEVAQIGRTLARALGANEDLVEA
                     ICLAHDLGHSPFGHSGEVALARLMKEFGGFDHNKQSLRIVTELEQRYPEFPGLNLTWE
                     VREGMVKHESEYDISDARDFNPDLRGNLETQIANVADELAYTTHDLDDGLRSGMITPH
                     ILEGISLWEILRETYKWQGHTLSEMERHRMIRQLVGLMVTDMLQATDIRLKDSKAKTA
                     LDIQKLKYNIIGYSEEMQRRNRELKDLLYKKLYRHYRVVRMQIKAERVISELFHAYQA
                     EPSMLPDHVQFFIEKRGLERTICDYIAGMTDRYAVEEHQKLFNPSEKP"
     gene            complement(54815..54898)
                     /locus_tag="PL000_01390"
     tRNA            complement(54815..54898)
                     /locus_tag="PL000_01390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54862..54864),aa:Leu,seq:taa)
     gene            55088..>56062
                     /locus_tag="PL000_01395"
     CDS             55088..>56062
                     /locus_tag="PL000_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HNC87575.1"
                     /translation="MADSNFYLGRLFDTKTAKVTTQPVEYDPADLTTHAVVTGMTGSG
                     KTGLCVALLEEAALQNVPAIIIDPKGDLTNLLLHFPDLLPQDFQPWIDPEIVRRAGTT
                     PERAAQEAAESWRDGLTEWGIGQDRLLALKNAAQFAIYTPGSDSGIPVSVLSSLAAPD
                     LDWETNREVLREKISSTVTALLGLVGMTDLDPLRSREHILLSNIFETHWINGKDLDLT
                     ELILQTQTPPFQNLGAFPVDTFFPPKDRMELAMVLNNILAAPAFETWREGEELDVQKL
                     LFMPDGKPRHSIFYLAHLSDGERMFFVTLLLSAVETWMRTQKGSTALRA"
     assembly_gap    56063..56162
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <56163..57736
                     /locus_tag="PL000_01400"
     CDS             <56163..57736
                     /locus_tag="PL000_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87576.1"
                     /translation="YGYLPPTAVPPSKGPLLRMLKQARAFGLGLLLATQNPVDMDYKA
                     LSNTGTWFIGKLQTERDKNRLLDGLESAAGGIPRAAMDKLISSLGKRVFVMHNVHEKM
                     PVLMQTRWAMNFLAGPMTRAQIRDLNLLADADSTRRASPMPAPAPKKESAPQPSVEAF
                     QPVSIPSASQPATPQPATLQSAPARQSTPASGFTTKPSIPAGIREYYLPQNYSLPEAF
                     QAAGRAMPSQAMIQGIIYRPSLLASAQVRLLDRKHGVDSELDKVVLVDEMDRRGIVRW
                     EDYSYGGPSLDRIEAMPAATARFSTIDAPLNNTKMIAALQKDFTDWVFRNSAVTARAN
                     QALKVFAGPDVSQAEFMKTCADTARDARDAEIAKKTASIEKKIKALEDKLAREERELR
                     QDEEDLQNRNIETGINGIEVVAGLFGVGRKKSVSTPISKYRMAQNAKAEVQESVDAIN
                     QYRKDLNALLRERDDMTAEINNRWGSVVNEISEVSIKPKKTDIYVNLFGVAWKPYYLV
                     QSGDETIELPAFGAE"
     gene            57832..58419
                     /locus_tag="PL000_01405"
     CDS             57832..58419
                     /locus_tag="PL000_01405"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNC87577.1"
                     /translation="MSIFILLLALAIWGIVHSFLASHFAKGMISLKTGGADFYRLTYN
                     VFALISFAPILYLMRTLPDQPIYQVASPWNLVMFGGQLFALLMLLIAFLQTDSLSFVG
                     LRQLFEKETTGALVTRGMYRIVRHPLYTFGLLFIWLTSTMTQNSLTVYCGATLYILIG
                     TYFEERKLLLDFGEAYAEYQRKTPMLIPGLVLGRK"
     gene            complement(58715..59245)
                     /locus_tag="PL000_01410"
     CDS             complement(58715..59245)
                     /locus_tag="PL000_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87578.1"
                     /translation="MSEVMQLLKCPSCGGPLEPPAGESTMKCLYCGSVVAIPESLRVQ
                     KQSTGYSQPSIFAGVDTSAMVGFGTQWGEVVQLAQSGNKAEAVKRYMALTGNDETSAT
                     YTVNHLAHTQMVTAQNIQQMYAPMMAQANETVKNVTRMSMWIGCGITALVTVIILVTL
                     IPVLIGVFASIMAIFR"
     gene            59357..60154
                     /locus_tag="PL000_01415"
     CDS             59357..60154
                     /locus_tag="PL000_01415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC87579.1"
                     /translation="MKRFAALTLTIFLIACTRSVTGSYTPIPLYSTKIPITPTLNPVP
                     VATETATPSCVDALLQVKIMPLGDSITYGEGDPNRGGYRTKLAEFLLEDGIAFDFVGS
                     QRSGEAVLADPDNEGHPGWRITNIKDAIASEGWLETYQPDIILLHIGSNDLRNGRGIY
                     ARDHLSALLDDILVRLPHVHVIVAQIIPTRWGSEIDHQIYNNAIPDVAASKGGQVSVV
                     DMQEILVKDDFMTLYHPSTSGYEKMAQAWRAAIHALGLQPNCAISQK"
     gene            complement(60272..61177)
                     /locus_tag="PL000_01420"
     CDS             complement(60272..61177)
                     /locus_tag="PL000_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC87580.1"
                     /translation="MKKFFKWLGIVLGSLLGVILIAFLGLALKGNSMLGKKYDVQVEK
                     ITIPTDAEAVARGEHWVMAECIGCHGHDLSGGEFFAAPFGYIDAKNLTPGNGGVGSDF
                     TDDDWVRALRHGVSSEGDSLLIMPAQFFRYYSDEDLGDIVAYLKTLPPVDKETRDPSF
                     NLMGKALMGAGAFGESTVVAAYIIDNPLENVSYPPAGVTAEYGEYIVNVSGCRDCHGA
                     DLSGGKSADPSAINAPNLTPGGELVAWEEADFIKAIREGEAKSGHKLDPAQMPWEHYK
                     FFSDDELKALWAYLQSLPKLATTIP"
     gene            61407..62786
                     /locus_tag="PL000_01425"
     CDS             61407..62786
                     /locus_tag="PL000_01425"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNC87581.1"
                     /translation="MTTLLIKNAYIVTMDNHQREIPEGGIFIRNGLIQEVGPVNELPA
                     TADEVLDLKGHVVFPGLVNTHHHFYQTLTRAVPAAQDANLFNWLKTLYPIWAKLQPDD
                     IFISTQTALAELALSGCTTASDHLYLFPNGSKLDDEIAAASEIGVRLQASRGSMSLGE
                     SKGGLPPDSVVDSEENILKDSQRLIEKYHDAKPGAMTQIVLAPCSPFSVTSDLMKQSA
                     KMAREYGVHLHTHLAETEDEEKFCMQMFGHRPVGYMQEVDWVGEDVWFAHAVWVNDEE
                     IKVFAKHNCGVAHCPTSNMRLASGIAPIKEYRKAGVNVGLGVDGSASNDGSHLLAEVR
                     NAMLVSRVKEGLTGYSLSNDPNRKLMTAREALHLGTRGGAAVLGRTDIGSLEAGKCAD
                     FFAVNLNRLGFAGMHDPVSAVVFGQPVNVDYTVVNGKFVVKEGRVVTIDQEKLIEKHN
                     KAAKRLLAG"
     gene            62804..63118
                     /locus_tag="PL000_01430"
     CDS             62804..63118
                     /locus_tag="PL000_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87582.1"
                     /translation="MFDSVLPIIFSWPFVVFSIIASVVGILYEMPLLVVVGALTIVPF
                     SYQLNNVQYFRGFALLLPVLQLFSAWALKEERNVWAWVLFAPTIAIILWLFVVNSIVG
                     FF"
     gene            63139..64929
                     /gene="ade"
                     /locus_tag="PL000_01435"
     CDS             63139..64929
                     /gene="ade"
                     /locus_tag="PL000_01435"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNC87583.1"
                     /translation="MTPSTKLTRSLVDVAMGRTPADLVIRGGKWVSVQSGEIIPHTDI
                     AIVNGHVAFVGHDASHAIGKKTKVIEANGRYLVPGLLDGHMHVESGMVTVTEFVRTVA
                     VRGTTGMFIDPHEIANVFGLKGVKLMVDEAQKQPIHVWVQMPSCVPSAPGLETPGSSI
                     GPKEVAEAMKWKGIIGLGEMMNFPGVFMGDKKMLDEMSATHAAGKTIGGHYASPDLGL
                     PFHGYVAGGAEDDHEGTRLEDAIARVRQGMKAMLRYGSAWHDVANQVKAITEKKMDSR
                     RFILCTDDSHAETLTVEGHMDRVLRHAIAEGLNPMAAIQMMTINTAEHFGLTKEMGMI
                     APGRWADVLLVEDLMNFKADVVIAKGQVIAEKGELLVKLPAVKYPRWATRSVHLKRPL
                     KAEDFVLRTRAAHGSDVDAHVIGVIENQAPTRHLRMKVKAENGEVKADMKRDLAKIAV
                     VERHRATGKVMVGLISGLGFTRPCAIASTVAHDSHHMIVVGTDDGNMAIAANELAQCG
                     GGQVVVMNGEVIGRVELQIAGLMSSERADVVAKKAGTVLDGFKTCGSELNNPNMQLSL
                     SALVVIPELRISDKGLVDVTNFEFISVLDE"
     gene            64937..65653
                     /locus_tag="PL000_01440"
     CDS             64937..65653
                     /locus_tag="PL000_01440"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC87584.1"
                     /translation="MSNFPFQRLQVDLAALIARTPAGQRLPSEPELAKQMGVSRATLR
                     EAMRAFETQGLIRRRQGSGTFVVGKMRGLLDSGLEVLESLETMAKRLGLEVSVSDLSV
                     DSLLADADAATALGVAVDSPLTRVKRVVRADNRPVAYLVDILPEAVLRVEDLPADFHG
                     SVLDFLLDRGDPLKTSRANVSAIGGTADVAKALEIQRGDVLLHFYSQLFDDEGRIVDY
                     SLSYFIPGYFHFHIMRRVGG"
     gene            65721..66914
                     /locus_tag="PL000_01445"
     CDS             65721..66914
                     /locus_tag="PL000_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNC87585.1"
                     /translation="MSIQEIQKRVQASREDIIEFMREIVAIPSMDSQLKDVGERIAKE
                     MNKLGFDEVRFDKMGNIMGRIGNGKRVIVYDSHVDTVGIGDPHEWEWDPFKGKIEDGI
                     LYARGACDEKGSTPGMIYGLAMARDLGLLEGWTAYYFGNMEEWCDGIAPNTFVEVDPK
                     VKPDFVVIGEPTKMLVYRGHKGRLEMKVTAKGKSAHAASNHLGDNAIYKLLPVIAGIR
                     DLEPKLGDHEFLGHGKITVSDLHVKTPSINAVPDEAVIFIDRRMTFGETKEMVKKQVE
                     DLIPAEFKDSVKLEELFYDEPSYTGFVFPVDKYFPAWALEAEHPLVQAGQEARKQIGL
                     PEAASGKWNFSTNGIYWAGKAGIPSIGFGPGDEETAHTVRDSVSLEDMVKATEFYAIL
                     PSLIK"
     gene            66960..67481
                     /locus_tag="PL000_01450"
     CDS             66960..67481
                     /locus_tag="PL000_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC87586.1"
                     /translation="MKTLEIRRHSIRDHAGDHLNQNGITLARLIGQNSGPFDRVVTSA
                     LPRAFETAIAMGFAVDEQTELLNTYGHAVEKEAPWPQTFDFYAEVIKHNGAPARYAGK
                     LAKFHASIMDFISEGRSALVVTHGGVVELSIVGCLPGFDFSQWGDPVDYCEGARLYWD
                     DKFVDAEVLRVNR"
     gene            67509..68504
                     /locus_tag="PL000_01455"
     CDS             67509..68504
                     /locus_tag="PL000_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC87587.1"
                     /translation="MKRKSVFFTSLLLVMALAVTVLAGCSSAKPKVALMLARGGLGDK
                     AFNDSANEGLQKAMQELGVEVKTFDYQQDTQSENVRKAAQDGYQLVIGLGSENSAAIT
                     EVAKEFPDTKFALIDATAEGSNVTSVTFSELEGDFLAGALAALLSENNKVAYLGGADV
                     LVIRRIQFGFEQGVKYVKPDAEIVVQYVAGKDDFSGFSKPEDANAIAAQMYADGVDLI
                     YAAAGGSTLGAIDAAKAAGRLIVTTGSDQRYLAPEVVVTSRTKNMNQAVFMLIESFTK
                     KTLQSGTIQLDYKSGGIGIAPLSADLVPASVLSAFEAIQTDLESGNIKLEPFVSQ"
     gene            68541..68924
                     /locus_tag="PL000_01460"
     CDS             68541..68924
                     /locus_tag="PL000_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNC87588.1"
                     /translation="MATIKNFEDIESWKKARELAGYVYTLTRKGGFERDFSLKNQIQD
                     AAGSVMHNIAEGFESGYDPEFVRFLKMARRSAGEVQSELYLALDTGYISQNELEKAYK
                     LAVDAKKLINGLISYLSKDQKTKDK"
     gene            68957..69946
                     /locus_tag="PL000_01465"
     CDS             68957..69946
                     /locus_tag="PL000_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC87589.1"
                     /translation="MQTNFRGRDFIGDLDFTKEEVETVLEVAWDLKRKRALGEPHPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHGAFIDSNTTQISHGDTPTEIGEIYGRYF
                     DGIAIRHCDYGDGNKYLNDVAKSSRAPVLNMQCDVYHPFQCLADLMTIMEKKGKDLRK
                     KKIVVSWAYAASYLKPISVPQSLILQMPRFGMDVTLAYPPEFPLMPDIVEQAKDEAKK
                     AGTAFEIIDSKDGMTEACKDADVIYAKSWGPLLTTTDKVEGKRLQDMYKNWLMDDAKL
                     KVAKEGAIYMHPLPADRDIEVTSNVLDGPQSVVFDEAENRLHAQKAVMALTMS"
     gene            70000..70950
                     /gene="arcC"
                     /locus_tag="PL000_01470"
     CDS             70000..70950
                     /gene="arcC"
                     /locus_tag="PL000_01470"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNC87590.1"
                     /translation="MTKFAVVAIGGNALIKDDKHVSVEDQEQALRETAIHLVDMIEAG
                     WDLAVGHGNGPQVGFILRRSEIAAKVEGMHEVPLDVCGADSQGAIGYELQQALRNEFF
                     KRKVNKKAATIVTQVRVDQADPAFQKPSKPIGSFMDEAEAKRREKEMGWSVVEDAGRG
                     WRRVVASPLPKEIVELDAVKTLLNAGQVVITVGGGGIPVVDRGNGELAGTAAVIDKDF
                     ASSLLAQSINADLFLIATAVEKVAINFGKPEQKWLDTMTVAEAKAYLEEGKHFAKGSM
                     APKIQAAIWYLENGGKEALITNPENIGRALKRETGTWIVK"
     gene            70990..72945
                     /locus_tag="PL000_01475"
     CDS             70990..72945
                     /locus_tag="PL000_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87591.1"
                     /translation="MKRIHPSLTLLAAAVLAYGLLIPQLGFYWDDLPMSWIRYQLGFE
                     AMTKYFSTNRPIWALLYQVTTRILPHLPIYWQVFALFWRWLGAVVLWNLAKELFPQRE
                     KFALTLGLLFLLYPGFNQQWVSYLYSHFFLVLFFFLFSQLLMLRGKTIPALVFSALNL
                     WMMEYFFVLELIRPFIIWTSLRDEVDPKERIKHTLNLWTPYLAVFALAVLSRIFIFNN
                     QIYQFSLKDELVKAPLATVLMLAQNVLSSFFVVTVAAWGQAFQFPNPLLDGPRTIAVY
                     AFVLLAVAALTLFGLWNQVSVKEGSGSTSVWRWALLLGAVMLVLAGPPFWLTNVPVSL
                     GFPANRATLSFMLGVSFVLAGLFDLLPQKFYGTVVVLFVMLAAGRQFLWSNEFRRDWA
                     SHKNMFWQMTWRAPGLEKDTIVLMNEELSFYADNSLGAALNLIYAPENHSDAIDYVLF
                     YPTNRESLSLSLDTPVNYDFLAGQFSGNTSQTVVFYYAPPKCLRLLDPEIDLENRLIP
                     DDTFLRDAALLSSTSPILSRQTSRMPAAYGNEPVHGWCYYFEKADLARQMGDWETVTQ
                     LGDVAFALDDYPNDPVERFVFIEGYAHTGNWERAVELSTASYKVSKAYVGPLLCRLWS
                     RIARETEDTSERKVTFDVVQSELECSP"
     gene            73051..74346
                     /locus_tag="PL000_01480"
     CDS             73051..74346
                     /locus_tag="PL000_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC87592.1"
                     /translation="MRKNVTRLSMFALILMLVLSACGGGQSAPAATEAPAGGDATEAP
                     AATEAPAATEAPAAEEAGGFQIPDVEEGKFNVAMVLIGPHDDGGWSQAHYEGLVYIEE
                     NVPNVHTAYIENVPEGADSEQVFRSLSRKGFNLIFGTSFGFGDPMAAVAEEFPDVMFI
                     HLTGINSNETNFGNLMGAMEDMKYLAGMLAGARAKADGNPKLGYMATFPIPEEIRLGN
                     AIALGMKKTCPECTMDVRWINTWHDPIIEKEAAASLFDAGAQVVFTGADTPAVADVAQ
                     EKGKWGVTYDWYGSCKVDACLTAPYWVWGPVYSQITEEVIAGTYKPGWQYFDADTGAL
                     GLYGFMEGQEMQPGVKDLDPEVIAMVQETLAQMLAGEFTRFDVFTGPIMDNQGNVVLP
                     EGQSLQQVDLDAFPEFGLPCSIDVCMKWWAEGITAELPQ"
     gene            74447..76036
                     /locus_tag="PL000_01485"
     CDS             74447..76036
                     /locus_tag="PL000_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87593.1"
                     /translation="MTQTTPSTPEIPLAVEMRGIVKRFPGVLASNHVDFELRAGEIHG
                     LLGENGAGKSTLMNVLAGLYRQEAGIIKVKGKPVDFYSPRDAINHGIGMVHQHFMLVP
                     SQTVTENVLLGLDDPRFFMRLPEYDAKVAAVGERFGLKVDPRAKIWQLSVGEQQRVEL
                     LKMLYRGANVLIMDEPTAVLAPQEIDGLFDILRSMIAQGKSVIFISHKLNEVSEIADH
                     VTVLRNGRVTASSVPSKGVSKQELARLMVGRDIVFFVDKKPVEPGEKVLDVKDVHAEN
                     DKGLPALRGLSLNVRKGEIVGVAGVAGNGQNELSQVITGLRKCKKGEVFLGGDKVSNR
                     DTLFGIQHGMSYVPEDRTHVGSAPNLSVTDNVIMKNFRKPPISKGGLLDMTAAGKFAN
                     ELKQAYDIIVPNVNTPVRLLSGGNLQRVILAREISEHPDFMVAVQPTRGLDVGAIEGV
                     HKLLLAQREAGAAVLLISEELDELLGLCDRVYVIYEGKIMGEVPIPIGKREEDLIETI
                     GLMMTGTPLDQIQKEGVGAND"
     gene            76029..77159
                     /locus_tag="PL000_01490"
     CDS             76029..77159
                     /locus_tag="PL000_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87594.1"
                     /translation="MTETTSPQKKSMGFKIRVEPRLDEPPAWHPVLVSLGAIIVALIL
                     GGILISFAGGDPIKSYLHIADASFGDTGVLSDTIVKATPILLAALACSVAFRMKLWNI
                     GAEGQFIMGAFGASAVVLAPLLAEDTPRWLFLTVMALAGMAAGAIWGFIPGYLKAKFN
                     VNEIISTLMMNYIAVAWINFWIFGVWSEGGFQMSPKFPNNAWLPRLLEYAKTYPALRG
                     LTTHAGLLLGIVAAVVLWLIVYRSRWGYEIRLIGDNPHAAKYAGINITGNIIYVMMLS
                     GALAGLGGMSEVSGVVHRLQTSPLAAGYGFTGIIVAWLAKLNPIVIVLVSVLFGALIL
                     AGREIQPSGVPKMIQGIILVSLIASDFLLRYRIRIVRADAEE"
     gene            77167..78135
                     /locus_tag="PL000_01495"
     CDS             77167..78135
                     /locus_tag="PL000_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87595.1"
                     /translation="MDPIIILQAGVATGTVLLFATIGEIFAERSGVLNLGVEGMMLIG
                     AMTAFSTTIATGNPWLGVLAAMLAAGLISQIHAFITITLQADQVVSGLALTFVGTGIS
                     LVLGEGLSKAGTVSLMPNFSIPLLSQIPFLGPILFTKQSVLVYIGYLLVPLTWYYINH
                     TRPGLNLRAVGEYPSAADSLGISVFRLRYFYVFIGGMMAGLAGATISLAVAPGWFSEL
                     TTAGQGWIAVGLVIFAQWDPVRAMFGSYAFGALRRLILDIQGPTILFGFNNPFYYNPY
                     WGFFLQMLPYAFTVIVLVIGSREAIRKRLGAPAALGNPYVRGERGK"
     gene            78284..79039
                     /locus_tag="PL000_01500"
     CDS             78284..79039
                     /locus_tag="PL000_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC87596.1"
                     /translation="MSSNDFSLMSIIQTKSVADQVEEILRERIRDATYLPGSRMPSES
                     ELSDEMGVSRATVRTVLAKLAVNGLIIRKQGDGTYVNARAQEASAHTGNLWDLVRLIE
                     NNGSKPFIQPISITRKPASEKEAQALAVEAGEPLLLFKRLFLADQIPVIYSNNLVPLS
                     FFRVSTDEIDGQLHFRDILRQYCHQEIGFVITDIRSTLVNDELRHLLRSELGPTVLEL
                     QMTFYSRNSQPLALGLNYFDDTALRLGLVQAWN"
     gene            79091..80224
                     /locus_tag="PL000_01505"
     CDS             79091..80224
                     /locus_tag="PL000_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87597.1"
                     /translation="MRSRLNALFDALLPVLATLAALGVGAIMLIFLKVNPVTAYKALW
                     DGAFGSSNAFAETLVKATPLLLVALGICISFRGDVINIGGEGQMIIGAVLATWVGLTF
                     TGLPGWLAVTLALVAGFLGGAIWGGIPGVLKAYFSVNEILSTVMMNAIAVQLMNFLLR
                     GPMIDPSQAELASKIPQTARLLDAFRLPRLAPTRLHLGALIAVVLAVAVYILLWRTTL
                     GYRIRAVGQNPNASRYAGIKVQRYMVLALLLSGAFAGLAGAVQVYGVNYRMITDGSSS
                     GFTGSAGFNGIVAALFGQLHPLWSIPASILFGALLVGANKMQRVVQVPSALVIALNGL
                     VVVFVVSSEILRKRRQRRRLADDETPPVAGSAANQSTQAETGT"
     gene            80221..81153
                     /locus_tag="PL000_01510"
     CDS             80221..81153
                     /locus_tag="PL000_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87598.1"
                     /translation="MISDLFTVTVLIGILASGIRLATPYLYAAIGETFSQKSGVLNLG
                     VEGQMLLGAFAAFYVSLTTGNLWLGLLTAMLVGALMGLAMAYVTVNLHAQQGISGIGF
                     YLFGLGMSDLLFQKLMGTVETVKGFPEIYIPGLSDIPGIGEIFFSQNILVYGAYLLVP
                     LAWFVLNKTTLGLKIRSVGENPDAADSLGVSVARVRYFTIILGGVLSGIAGASLSIAL
                     LNVFQQNMTSGLGFIAVALVYFGGWRPVGVLGGALLFSLINSLQLWIQVLGIPIPSDI
                     AVMMPYVLTILVLVATVSRVRSPSALSKPFEREN"
     gene            81266..82327
                     /locus_tag="PL000_01515"
     CDS             81266..82327
                     /locus_tag="PL000_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HNC87599.1"
                     /translation="MKKFALSLVIAFVLLLSACGTPAATEAPVVEAPATDAPAEVPAA
                     TEPPAAPAEPFRVAVVMPSATNDLAFSQSMYDALVRIQEEMGGPEAFEFVFSEGMFVV
                     DDAAAAIRDYATQGYDLVIAHGSQYGSSLQEIAPDFPETSFAWGTTADTFGQPNIFAY
                     EAASQEGGYVNGVLAATLSQSAVIGVVGPIETGDAKLYVDGFKAGVAATNPAVTVNVN
                     YIGSFSDVALASEAATTHISAGADVMTGTAQMVVGAIGKAEENNVLWFGTQSNQSSLA
                     PKIVVASQVYHWEVTLREIIALIKEGTLGGTSFKANLANGGEVIEFNPDYALSDEAKA
                     LAETTIQGIIDGTIKITLP"
     gene            82407..83960
                     /locus_tag="PL000_01520"
     CDS             82407..83960
                     /locus_tag="PL000_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87600.1"
                     /translation="MTESTKLPSGKPRIETIEMRGITKRFPGVIANDKADFDIHAGEV
                     HALLGENGAGKSTLMKILYGMYRPDEGEILLNGKPVTIASPNDSINLGIGMIHQHFML
                     VPTLTVAENVALGLPSTRGALTDLDRVSKRIVELAGIYGLKIDPSAYVWQLSVGQQQR
                     VEIIKALYRGAALLILDEPTAVLTPQEVDELFVIMRQMVKDGHALIFISHKLHEVVEI
                     SNRITVLRDGRRIGTRPTSETTKQDLANWMVGREVGFTPDRGKVEIGEARLKLEKVEC
                     GSDRGTPGLRGVSIEVRSGEILGIAGVSGNGQRELAEAITGLRKVTAGRVFLEGKDIT
                     NFAPGDITELNLSYIPEERMRDGMIKEFTIAENMILREHHKMPYSRMGFLHLGDISKH
                     ADELIAKFHVKTPSRETHAKNLSGGNIQKVVLAREISRNPRAIIAAQPTRGLDIGATE
                     YVREQLLVERRKGVAVMLISEDLDEILALSDRIAVIYEGQVMDIVPRAEATPERLGLL
                     MAGVHPEVG"
     gene            84177..85049
                     /locus_tag="PL000_01525"
     CDS             84177..85049
                     /locus_tag="PL000_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNC87601.1"
                     /translation="MNLWQEYKRPASVTEAIQALTSASGPAMPIAGGTDLLLDLKQGR
                     HAPIHTLIDLTFVTEMTALELRGNELFIGAAVPVNRVALDPLTGTHAQALVEACNLIA
                     GPQVRNTATLGGNVAHALPAADGTIALTALNAQAEVAGLSGTRRMPFTSLFLGPGKSA
                     IDKTKEIIVGFHLPLSTPHSASCFKRIMRPQGVALPILNCSVWLERDAEMVKDVHIAV
                     GPGSSVPFRATDAENALRGKSLNEETFNQTLDALLGQAQFRTSARRATADYRKHIVGG
                     LFKDVLDTAWARTS"
     gene            85092..85511
                     /locus_tag="PL000_01530"
     CDS             85092..85511
                     /locus_tag="PL000_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNC87602.1"
                     /translation="MQQRNFPVLQNKHAVPLLEKDITDKIIGAAIEVHRALGPGLLES
                     AYQLCLAQECRLQKLSFEEQIALKLNYKGVEMDGGYRIDFVFDKRVVVELKAVEEVLP
                     VHEAQLLTYLKLAGIRVGLLINFNKPVLKDGIYRRVL"
     gene            85581..>86085
                     /locus_tag="PL000_01535"
     CDS             85581..>86085
                     /locus_tag="PL000_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC87603.1"
                     /translation="MNNQISLIVNGKQHIIEAVAGETLSTLLRERLRLTGTKIGCEEA
                     ECGACTVLVDGQPMMSCVYPAERADGKVVTTIEGLAQRVHEEMKLHPLQEAFVEHGAV
                     QCGFCIPGQIMTAYALLKRNPNPNSDDIRFALKDTLCRCAGYPSIENAIIAAAQALRT
                     GEPVQKPT"
BASE COUNT        19952 a        21215 c        23029 g        21589 t
ORIGIN      
        1 aggccgcgca tgagggtcat gggcacatgt gtatgggcgt tgaccagccc cggcatgagc
       61 accctgcctt tgcaatcaac gatctcgctg gctgtatatt cacttttgat ttcggcttcg
      121 ctgcccacag cgatgatcga atcgcctttg acggcgactg cgccggggtc atattgattc
      181 aagttccgat ccatcgtgag gacaatggca ttgataaata gtgtatctac ggtttgtgtc
      241 attcattctc cttttgcaag tgatctgttt ttggagtgct aaatttttcc gagcttgttt
      301 ctgattttat ctgccccagt tcaaaagaat tcccaaggcg gcgaggtttt catcgaaatc
      361 tgttcgggtg tgtgtggcaa gttccatgtc cactgaaacg gcgccgagtt ccacggcata
      421 atcggcgagg gtgcctgtga aaatgcatcc cagatccagc ggtgggaagg agtaatcggt
      481 cacttccgcg agggcgcggg cgaaatctgt ggattcttcg tcccagggtt ctccgccggg
      541 gaaaactccc agcgcagcac tatggtaact gatcagcgcc tccacgcggt gcgattgcag
      601 gaaacccatc aacgcgcggg tttcaggttc agagccaggg cctgtgccgc ctgtggtggg
      661 gcggtagtcc caacagccgt cacggtccca ttctgccgcc cagttggtgg ggaagtttcg
      721 attcaagtcc acgccatggt cgttgacacg gccatcgtac ccgtggtcac gtgcatcacc
      781 gtcggggttc aaattgcgca ggatgtaaag cgtgacatca ctggggatga tctctgggtg
      841 ttcataaacg tgggtgatga gttcatcagc cagcgcaatg gtgttccatt cgtagccgcc
      901 gtgaatgcca gccacgatca tgcgctgact ttcgccgttg ccaaaggtgt atacctctat
      961 ggggcgccct gcaaccgaat agccaatgat ctgcggacga attccatttc catttttgct
     1021 gatgagcaca aagggagtcg gcgtttggaa tgtgatgcgc gtggcaagcg gatttggagt
     1081 gacggttggc aggaacagcg aggaatttgg gtcaggtgtg aaggtgttgg gcagggctct
     1141 tgtagaaatg ggctgtgcct cctgcgcaga ggcaaaggaa gtggtcatga ccacataccc
     1201 aatggcaaca acggcgatgc cgcccagacc gattagattc agcgtccatg ccagtgtgac
     1261 ccaaatggat tctttgcgtt gtgggcgctg tgcagacatt agttgaattc ctcaagggtc
     1321 attctcaacc agttcaaggc caggttcatt gcccatttgg gcaggctgcg cggcgggccg
     1381 ccgtaggtga tgcggtgggt tttctctcca cgcggcgtga tcatcgccat ttctgcggag
     1441 cggtctgcgc tgtagacgga aactcccaat gcaatctctg cttttgattc tgcccgggcg
     1501 gcgcgtaatg cttcgaggag ctgggcaggt tcaaggttgg agaaagaagc ggtgtgcgga
     1561 atcttccgcg cgagaattcc atccagcccc gattcgatgg cggtcaaggc ccatccgcgt
     1621 tttgctacaa ggtcgagagt cacttcttcc aatttatctt catctgcgcc aaagaccaca
     1681 ttgccgagcc tttcgcgcac ctgcgcttcg atgacggcga tcatttcatt ggcttcgttc
     1741 tcgttcctgg cttttgcggc aatgcgtaca tccactacgc ctgtgtgtgc cgccagcccc
     1801 acggtggggt tgctgagggt ttcgaggtcg gcaatctttt catcgatcat gccttcacca
     1861 agcccggcac aatgcagcac gcgtacttta atgatctcgt tgaggttgaa gcgtttttgc
     1921 aaatagggga tcatggattc gtgcaggatc tgttccattt cactgggcac accgggcagg
     1981 gaaatgacag cattgcgttc ggtttccaca ataaaacagg gggccgtgcc aacagggttc
     2041 ctgatgccaa gggcgccttt gggcacgtag gcctgccgct tttgattttc ggagggtttg
     2101 cgcccatagc gagcgatggt ttccaccacc tgctcccaaa ggtcttcacg gaattccgtt
     2161 tccacgccga cagctttggc gactgcttct cgggtggggt catctacggt ggggccaagc
     2221 ccgcctgtgg tgatgacgat gtcggcacgt tccatcgagt tgtggatcgc gcttgcaatg
     2281 cggtcgatgt tatcgccaat ggtgatggtg cgatacagat ccacacccat gctgcggagc
     2341 gtgcgcgcaa tataacgcgt gttggtatcc acgatttcgc cgagtaagat ttccgttcct
     2401 atggtgatga tctctgctga tgtcattttg tctcacataa gtttctgata tgaaaaagga
     2461 tgcaaacatc cttttgtgca gctggttaat taaaccacat tgtactcttg aattaatcgg
     2521 ccttcttcga agcgttttag atccaacatg gaaacatcga gggtggagaa ctttccatcc
     2581 aaaataaact cgctcatcaa tttacccgaa acaggtccat gcataaaacc atgcccggag
     2641 aatccagaac acagaaaaaa accatccaca tcagtttttc cgtaaatcgg gtgtgcatcg
     2701 ggcgtcactt catacaggcc agcccaatgc gaagcccgac tggccttctc cattagtggc
     2761 agccgttcaa ttgccgcttc aagattcaca agctcaaagt catcgtccac gttttggtcg
     2821 aagccaggtt tttcattttg gttgctcatg cccaccagca agccttcgcc ttcgcgatga
     2881 aaatacagcg attttgcaaa atcaattaca aagggaaaat cggcggggat ttcctccaat
     2941 gggttggtgg tgaacatttg cctgcgaaga gggacaatgg ggatcgtgac gcctgccatc
     3001 gctccgatca aacccgacca aggccctgcc acgttcagga tcatgcgcgt cttgattccg
     3061 ccccggcttg tttgcacacc gtcaaccttc cctccgctga cggtgactcc tgtaacttca
     3121 acatccgaga tggcttttac ccccatcttt tgtgcggcat tgatgtatcc cattaccacg
     3181 ccatttgggt caacagtgcc atccttctga ttaaatgtcc ctgcaattgc atcattgaat
     3241 ttcatcaacg gcaatcgcgc acggacttca tctccgctca gcaattgggt tggaatgcca
     3301 agcctgtttt gcagggccac gttatgtttg aattcggcaa ggtccttttc gttcgttgct
     3361 acaagcaggt atccgcaagg acgatagctc acttcctgcc cgatctcttc cttgaagcgt
     3421 tcgatcatcg gcaggctttc gatggatagt tttacattga tctctgttga aaattgatag
     3481 cggacgccgc ctgcgcatcg gcctgtggcg cctgtgccaa aatacttttc cttttcgatc
     3541 agaacgatgt ttttaagtcc gcggtgggcg aggtgatagg ctgcgctggc gcccatcacg
     3601 ccgccgccaa taatgacaat gtctgcagaa gtgggtagtg tatccatgag atgtgccttt
     3661 attttgaaaa gacccgcttt tgcaagcggg tctttgacag tacatgagct tctatgtgtt
     3721 tattccacgc cgaggtaggt cttttgaact tcctcgttct tcatcagctt tttagcgtca
     3781 tctgccagaa cgatttcacc tgtctgcagt acgtagccgc gatctgcaat ggaaagtgcc
     3841 atcaaggcgt tctgttccac cagcaggatg gtggtgccct cgtcgtttag cttcttgatg
     3901 atgtcgaaga tcaattccac gagtagtggg gcaagaccca tcgaaggctc gtcaagcaga
     3961 aggatttttg gctttgccat caacgcacgg cccatggcga gcatttgctg ttcaccgcca
     4021 gagagggtgc cgcctttctg gctgatacgt tccctaaggc gtgggaagag ggttaacaca
     4081 tgttcgaggt cttctttgta ggaggccaga tccttgcggg cataggcacc catctcaagg
     4141 ttttccagca cggtcatgcg cgggaagatc atgcgccctt cgggactctg cgccacgccg
     4201 agtgtggcga tctcatgcgc gggaagtttg ttcaattcta cgccatcgag aagtaccgat
     4261 ccgctgcgtg gtttgttcag cccttgaatg gtgcgcagtg tggtgctttt tcctgcgccg
     4321 ttcgagccga tcaatgtgac gatttcgccg tcgttgaccg taagcgaaat ccctttcaag
     4381 gcgtggatat taccgtagta agtgtggaca tcttttaatt caagcatgtt gtgttcctcg
     4441 cctttatttt gtggcagtgc ccttgcccag ataggcttcg atcacgcgtt cattcttctg
     4501 aacttcggaa ggcgaacctt ctgcgatctt ttcgccatag tccagcactg aaacgcggtc
     4561 agagatggcc attaccacgc gcatatgatg ttcgatgagc aggatggtca ggtcaagttc
     4621 cttgcgcagg cgttgaatga agttggtcag atcaatggtt tcattcggat tcatgcctgc
     4681 tgttggctcg tcgagcagga gcagtttggg ttgtgaagca agcgctcggg cgatctcgag
     4741 gcggcgctgc aaaccgtagg acagattctt ggcggtcaca ttcgcgtcac tgcggcgcag
     4801 tcctacaaat tcaagcagat ctaatgcgcg cgcgcgggca tcggcttctt ctttcatggt
     4861 tgcgggagtg cgcaaaacct gcccgatcag gttggaggtc aggcggcaat gctggccgac
     4921 cagaacgttt tccaaagccg acatattcgc gaataggcgg atgttttgga aggtgcgggc
     4981 gattcctagc gtggtgatat cgaacggttg caggccagaa agatccttgc catcgaattt
     5041 catttcaccg ctggtaggtg tgtaaacgcg ggtgatggta ttgaagaagg ttgttttgcc
     5101 tgcgccgttc gggccgatca cactggcgat catgcctttt tcgaggttga aatcaacctt
     5161 gttcacggcg gttaaaccgc cgaatgtcat ggtaattgct ttggcttcaa aaagcatggc
     5221 agtctctcct accctttctt ttctgcggat gcttcgtctt cttccttgtg caattccgcg
     5281 cggatttgtg catttggaat caaaccttca ggtcgaacgg ccatcatgat gacaagtgtc
     5341 aagccaaaca aaaggaacct gtacaagatc gggttcgcat tgtctttgac tgccagttga
     5401 acaacgggat cgctaattga aggaagaacc gtgtgtgtta aaagagagga aaggaagtct
     5461 ttcagcgcag gcaggaagag gcggtctgcg gtcatcaata caagaccacc gacaattacg
     5521 ccgttgatat tgccgatacc accgagaatg accacgcata gaatgatgat ggatgcgctg
     5581 aagtcgaaaa cacttggaaa gataccgcgg atataagctg catagaatgc gcctgcaaac
     5641 cctgagaagg tggcacccat ggcaaacgcg gccagcttgg tgcggacggg gttgatgccc
     5701 atttgtgcgg ctgccaattc atcttcacga atggccatcc aagcgcggcc aaggcggctg
     5761 tcgcgcaggc ggcggatgat gaaaatcgaa agcaggatga tcgcgatgat caggaaatac
     5821 caggttaccg ggttgctcga ttcaaattct ccaataatgg gaagtgaggg ccttccaatc
     5881 ggactgatgc ctttttcacc gagtgtcgca tcaaaatgtt ctgtgaagac gatacaggtt
     5941 gtattggctc caaacatggg aaggatccag caggtgagcg gttccttgat cgtcacatca
     6001 atcaggttct tgaaaagcac tgggacaatt tcaccgaacc ccaacgtcac gatggcaaga
     6061 tagtcacctc tcaacggtag agttggtgca ccaagaatga tgccccacac acttgccatt
     6121 gctgcagcaa tccagatcac cagccagaaa ttccactgaa tattgtgaac gggtgaagag
     6181 agaatacctg ttgtgtatgc gccaatcgca aagaaagccg cataaccaag gtcaagcagg
     6241 cctgcatacc ccaccacaat gttgagacca aggcccagaa tcacgaagat caatgcaaag
     6301 atgatttgtg ccgtccagcg caaagcagta gctttatcca caaaggggaa aacgatcaca
     6361 aggagtgcca aaagaatcat cttggcatat tgttcttgtt tttcaggaag tccgcgcagg
     6421 gaggccagca gagtggtgaa gatgagcgca ccaaagaagc cgatcagatt ggcattcatt
     6481 gcaatatctg ctttggctgg ctttttgccg atcgcttcca tgacgaagtt gttttgatac
     6541 aggcttaatc caacaagcag gactgccgcg accaatgccg acgggagaac tgccagggcg
     6601 attttgagtg gatccttgcg ttcgaagtta ttgccaagcc ctaatccaag gccaaccccc
     6661 atcagtacgc caaggactgc gaccgaccat cctccaaaaa gaaaggtcag caagccgccg
     6721 atgatgccga aggtaattcc aggtcgaata agtttcatga atgtgcctcc taggccttct
     6781 gtccggtgtt ttcaccgagc aagccggtcg ggcgaaatag cagaatcaag accagtgtgg
     6841 cgaacaccca cgcatttgtc cagcgggcgt tgagataacc gtcactgaaa gaagccaaca
     6901 gaccaatcaa gaatccacca agcatggcgc cggtgatgtt gccaataccc ccgagcacag
     6961 cggctgtgaa ggattgcaaa ccagcacgga agcccataaa ccaccaggcg gtattgttgt
     7021 acaagccagc cattaaacca gctgcgccag caagaccacc accaatcagg aatgtggctg
     7081 tgataaccca gtcaatatca ataccaagca ttttcgccgc atcgcggttt tgtgcagtgg
     7141 cacgcatggc ctttccaagg cgggtgcgct gtacaaacaa atacaggcca gtcatcaggc
     7201 caacggccag aacgatgacc aacaggtctt tgatcgtaaa ccgaagtgca agcccacttg
     7261 aagccagaag atttacatca atgccaaggc tggtgaataa cgtgccaagc atatcgggaa
     7321 atactttttg tgatgcgcca ccagtagtac cgaaagacgg caccaaccag gcttgtgcag
     7381 cccacatcaa gccaatgttc tcaacgataa aagacatgcc aatggctgaa atcaatggcg
     7441 caaggcgggg ggcgtttcga agccgtttat aggcgagtct ctcaatagtt gcattcagcg
     7501 tggcacagac tacgaccgcc acaacgagtg ccagaattaa tcccaaaata atcttggcag
     7561 gatctttact atccagtgtg ccggtgacag acaatacagt aagggaggta aaaacaccaa
     7621 tcatgtaaac atcgccatgg gcaaaattga tcaactcgat gatgccgtac accatcgtgt
     7681 atcccaaagc gataatggca atgattgcgc cgttggcgat gccgataatt aaaaattgaa
     7741 ccaactgcag tgggttggct tttatggctt gcacaattgc aggtacgtca agcccggcga
     7801 ttgggccaat cagaaaaacg atgtaaagaa caattgctat tgtgccaagg cggcgccagt
     7861 ttattttctt gaggttttcc atgattctcc tctacggtac cttcaacttc ccttgtacct
     7921 gttaagaata gtgggggcgg caaagatgcc gcccccagaa cttgctagag acttatttga
     7981 aggtgccaac aggagcgtaa gcgccgtctt tgacctgata gaaggtcatg tcggtcaggc
     8041 ttgtgtcacc gtttgcatcg aacgaccagg tgccgagggc gccgtcaaag ttctgaatgg
     8101 cgaacacggc ggcgttgatg gcagcgcggt cggagggatc gccaccggcg gcgcagacgt
     8161 cttcaatcgc gcggagcgca acgttcatgg tttcgtagcc gtacacagcg tagggctcgg
     8221 tcaaaggacc aaacttggct tcgtaatcag ccttgaactg cttaccagct tcggggagtt
     8281 cttcgaaagg aacaccagcg acggaagcat acacaccagc ggccacatcg gcgccagcgc
     8341 catcaatgaa ggcctgggtc tggataccat cgggaccaac gaacttgacg gtttcgttgt
     8401 cacccatgat ggcgaccttg tccttcagga gctgggaggc gttgttgtca acgaccatac
     8461 caacgtaaat ggcatcagga gcgccaccgt tgttgctggt ggagatcttg gtcatgaggg
     8521 ccttgtaatc agcagccttg gggtcaatac cttcgtgacc aacaacggtc aaaccgattt
     8581 cattggcggt ggcttcgaac acatcagcaa tacccttacc gtaaagttcc tgatcgtcga
     8641 ggatgtaaac ggtggtggcg ccgagagact tcacgaagtt ggcggcaact ttaccctgaa
     8701 tgtcgtcagc agcaactacg cgggcgtagg agcgggtgcc ggtcgggtag taggaatcgg
     8761 ttacgccggg gaggtccttg tcagccttgg tcaaaccagg gttggtgttg gcgggggaga
     8821 tcatgaccaa cgggccagca gcgttcaaaa tcggaatgga cagcttggca gcgccagagt
     8881 tgaacgtacc gaggtaggcg atgatggcgg ggtcagcagc agccttgttg gcgttttcgg
     8941 tctcgacagc gggatcccac ttaccgagag cggcggaggc atcatcccaa gcttcgtatt
     9001 cgagggtgac attgccacca caggctgagt agttagcctg ttcgaggcgc aactgcatgg
     9061 cgttcacgat ggtctgggtc tgggtcaggg aggaaccagt catcgggagg ctggagacga
     9121 ccttaagggt aacggcttca gcggcagggg cttcagtagc ggcgggttct tcggcggggg
     9181 cttcagtagc ggcaggctct tcagcggggg cctcggtagc ggcaggagcg ccaccaccac
     9241 aagctgcaag catcatgctg gcaagaacca acacggaaag taaagcgagc aaacgcttat
     9301 tcatttttct tactcctcca gaatatatta ggtgaattcg acgtcccgca atgatgcggg
     9361 gttacaaaag cagcggacaa tgcttacgat gtcagcctca cctccttttg ggtaaagtac
     9421 ttcctctttt aaaagaaagc ccgaaaacct ttataacgat tttcgaagcc cactctaaaa
     9481 cggcaagctt ttcatttttt ttattaaacg gcaatgataa gggggttttt ctctaaggtc
     9541 aagagcacgg attttatttt ttatcgtgtg gtttatacaa caattgttca cggatgcgca
     9601 agtctgtgtt acgaattttc attgcaaggt cagccgcaag attgtacatc aaccgatacc
     9661 ccaattgtgg gtaggtttcg caaagcataa tgagtttgtc acgttgtatg acaagtaacc
     9721 gagtgtcttt ttgggccgcc cgagcagatg cggatcgtaa tccttcatcc accagtgcaa
     9781 cttcgccaaa tgattgacct ctgcgaaggc gtgcaacggc agtttccttt ttatcagatt
     9841 cccccgtggc accacggttg atcaaaatat ccacctcacc ttgtgcaatc acgtacaatt
     9901 ccttgctgcc actattttct tgaaagacga ggtcaccagc attgaacaca acttcctgac
     9961 aaaggtttgc caccagttcc aattgagtgg gtgtgaactg gtaaaaaata tcgccttgct
    10021 tgagaaagtt gaccatgcta ttcataagtt gagagtttag cataaataaa tatgatgtgc
    10081 aactgttttt gcgatgaaat caaaagacgc ctccagtcta cgggtaagtc agaatcaacc
    10141 aatcaagagg atccacttgg attccgttta cccataactc ccaatgcaga tgcgccccag
    10201 taacgcgccc tgttccgcca acaagcccaa tcacttcgtt ctgttgcacc acttgaccaa
    10261 cctgtacatt gatctgggat tgatgccaga agccgctgta aattccccag ccgtgatcaa
    10321 tgatagtggc atttccgcgt actgttaacg ggccagcaaa aacaacctga ccagccgccg
    10381 gagcagtgat cggcagccca tcaccaccgc cgaaatcaag cccggtgtga aaacctgtga
    10441 tttgcagttc tgtaccttgc ccaatgtaag tgcgacgatt gccatatctg gaagtgaaat
    10501 aagtggattc ggcgtatgcg atcgcaggag aaataaaatc accgctccaa tatttgatcg
    10561 gattggaagg cagagtaagt tgaattaatt gctgcaattc tggttcggtg gaggcagggt
    10621 caatggtggc agggtcaaca tatagcagcg gatcatcagg ataattaccg gaaatgatca
    10681 aaatgaactg ctcaaaagat tgtttgctcc catctggcag cgtggcttct aatcgtaaag
    10741 gataaacccc gggcggtaat aaggcatgta ccccttgaag agctacttga gtcccatctt
    10801 ccattggaaa aaaatgtaga gcgtggtcaa caaggagtcc gctgagtgtc acatttggaa
    10861 tcgtttttac tttgataaca cccgtgccgc cctgtttgat tggtaggtca cgaatctctg
    10921 cactaataaa agcagcaggt aaaccgctgg tcgtttgttc tgcgttatct tcaccaacgg
    10981 taaatagtgt atcgcctggc atactatccc aggagcctgc cagatgattg ttgtgggaaa
    11041 gggtccatac atctgtgttt tgttgaacag ccaattccag cagtgattgt cccttggtgg
    11101 gagagatgcg tttgttcaaa ccctggctcc ctgattgcac ggggacgatc atacttacac
    11161 ccacataaaa ttcactgggg cttaccacgt gatttaattt tttgaataaa gtttctgata
    11221 cctgcgtacg cctgataagg ctgcgataag agtctccaaa gttgacaact tcagtgttca
    11281 acgtccctgt gatgccactt agcccgggga tgatgagcgc atcccctgcg ttgagttgat
    11341 tggcattggc aattccattg actgccatga gatcggccaa actgacactg aaccgttctg
    11401 caatggagga aaggctatca ccaggttgaa caatatagat gggaccgccc gtatcctggg
    11461 cttgtgcagg ttgaaaggga aaaaacaaag atagaatgag aattagcaaa atgatatttt
    11521 tagacatggt ttttgaagga aatactggag tgagtgcagt ggtttattgc aggttcttga
    11581 attcgacctg atcgccaatt tcgtaatccc cgaagcgttg cgggtgaatt tcaagggtgt
    11641 atctggcatc tgcttttgga aagtaggctg gatgccatga tttagcgatg atcttatcta
    11701 caactttcat ttcagaattg atccaaaata ctgccagatc gaaggggaca aagaacatat
    11761 ggatggaagt gtcgaggcgc gaatctcgtt tttcgaccag aagcaaaccc tcgttaagtt
    11821 cgaggcgttt acggaacatg agtccgcgca ggcggcagga gaatgaatcg caatatccca
    11881 cacgggcggg ctgttcgatc atgcgattta agttgtgtac aaaaatggga cggggcatgt
    11941 gggttatata aaacaggacg gcatttccgc cgccctgcca aaagataaaa gaatttcagg
    12001 atgatagttt ttggccgaca actttttcga cactgtccag cagttcttgc gggccgaacg
    12061 gcttggcgat gtagtcatca actttggcga tgtgcaagcc gaggaccttg tctatgtttt
    12121 gtgctctggc tgtgacaaca atgacgggaa tttcacgggt ggatgaatct gctttcaatt
    12181 gttgatatac ttcccacccg tccatttcag gcatcatcaa gtctagcaac acaagatcag
    12241 gatgcaggtc gcgaaccatt ttgaggcctt cttttccacc aggggcgcca tgcacgtcaa
    12301 acccgcgccg cccgaggatg agccgaatga gctcaatcat ttcaggttca tcttcaatac
    12361 atacaatatg tttgatagat gtattattca tttttctcct gtttcttaaa ataagtttac
    12421 cataaaaaaa atatggattt gccgatgaaa atcatcacat ggaacgtcaa tggtattcgt
    12481 gctgcgcttg gaaaaaatgc gctggattgg gcctttgctc aaagccctga ttttttgtgc
    12541 ctgcaggaag taaaagcccg ccaagagcaa ctggatgagg aacaactcgc caaattaaaa
    12601 ctttcttttg tctggaatgc cgcacagcga ccgggataca gtggggtaac gacctttttc
    12661 aaaaatcaac ccgatgaagt tgtaactggc atgggcaggg aggtttttga tgtggaaggc
    12721 cgtgtcattc aaacccgttg ggctggttgc cgtctattca atatttactt cccgaatggc
    12781 acccgtggac atgaccgcgt ggaatataaa cttgatttct acgctcattt gcttgagttg
    12841 tgcgatgccc tgcatgaaaa aggagagaac cttatcatta ccggtgattt caacaccgca
    12901 cacatgccca ttgatttgaa aaatccgaag caaaacgaga agacttcagg ctttttgcct
    12961 gaggagcggg agtgggtgca aaaattttta gatcatggat ttgtggatgc ttaccgcaag
    13021 ttgtatccag agaagatcga atacacctgg tggacttatc gtctggatgc acgaaagcgt
    13081 ggtattggct ggcggttgga ttacttcctt gtctctgaaa aattgatgcc ttttgtaaaa
    13141 gatgtgatcg tccacgataa agtgatgggc tccgatcatt gcccggtgga attggttttg
    13201 gaaggggagg gcttttaatt tcggtcgagg ttggtgtcat cccacgaggc agggtcgttg
    13261 agtgattcga ggtgggtgaa gaccgtgaca tttgggaggg ctttgcgaat gtcagactcg
    13321 atgctttcca gcagtcggtg tccgcgctga acggtccatt tgcccggaac aaggatgtgt
    13381 accgacacga atctccgtgc gcctgattgg cgggtgcgaa gggcgtgata ttccacatta
    13441 ttttttgtgt atgagctcag gacgtttttt acgagttttt gttcatctgg ttcgagagct
    13501 gtatccatta atcccagtgc tgacatgcgc acaattcgga atccagacca gatgatgttc
    13561 cccgcaacca cgaacgccac gatggggtcc agtcgttccc agcctgtcag caccaccagc
    13621 ccaacacctg ccaacacacc gacagaggtc catacgtctg tcattaaatg atgggcgttg
    13681 gcttcgagtg tgatggagtt atgttttttg ctggcctgca gcaaaaccaa agcaacaagc
    13741 agattggcaa gtgaggcggc tatagaaaca cccagtccca gcccaacctg ttcaagcggt
    13801 tgtgggttga taagccttgg aatggctgca atgatgatgc tggccgccgc gatcagaatc
    13861 aatgcacctt caaccccgct ggagaagtac tccgctttgg tgtggccgta agcgtggtct
    13921 tcgtctgcgg ggcgggcagc gatggtgagc atggcaagtg ccatgacagc accaaccagg
    13981 ttgacgatgg attccaaggc atcggagagc aagcctacgg acccggttag aaagtaagcg
    14041 atcgttttta gggtgatcgt aaaaatcgct gcgccaattg aaagccaggc aaatttggtc
    14101 aggaacaggc ggtcgttggg tgatgaatgt gccatactgg tgattctatg tcgaagcctg
    14161 ttttgcggtt agtgacattt atcctccata tatgagtttg tggaggaatt gtgtaggttt
    14221 ttcggggagg gagatttttg attgtttttg caagcagttt tcggctaaaa atgaccgcat
    14281 tatttcagtc aaaaatggcc gtattagaat atcatataat atttacccag tacgcgtcat
    14341 ctaatggtat ggcttggtat aatctgcccg tacgtcatat ttggaggatt ccgtgaattc
    14401 ccgtagtcaa tcgtttttcg tgtacttcct tctgatcgtt gccattggcg ccatgtttta
    14461 catgggtatc agcgaaaact tgaatacaga agagccgctg accattaatg aggtggctct
    14521 ggctgtgcaa aagggtgaag tcgcccgcat tgtgatcggc agcgacgata ccgttcgtgt
    14581 gattttcaat aacggtgtgg aagaaggcgt tgagtctcgt aaagaaccgg atgccacatt
    14641 tgtggatcag cttcgtagtc tgggtgttac gacggaacaa cttgcccctg aaaatgtagt
    14701 catcgaagtg agcgctccct cggtttgggc gggtgtttta agcggtgcca tttatatttt
    14761 gcctgttatt ttgatggcgg gtgtgttgtg gtttattttc cgccaggcgc agggcagcaa
    14821 taacgctgcc atgtctttcg gaaagagccg tgcgcgcatg ttcagcgggg agcatcctac
    14881 tgttaccttt gctgatgtgg cgggcgctga agaatcgaaa caggaacttg aagagatcgt
    14941 tgaattcctc aaggagccgc aaaagttcat tcaactgggc gcgcgcattc cgaagggtgt
    15001 tttgctcgtt ggccctccag gcacaggcaa gaccttgctt gcaaaagcag tttccggcga
    15061 agcgggtgtg ccgttcttct ccatctctgg ttctgaattt gtggaaatgt tcgtgggtgt
    15121 tggtgccagc cgtgttcgtg atcttttcga tcaggccaag cgtcactctc cgtgcattat
    15181 ctttatcgat gaaattgatg ctgtgggtcg tcagcgtggt gccggccttg gcggttcaca
    15241 cgatgaacgc gagcagacct tgaatcagat gctcgttgag atggacggct ttggcactga
    15301 taccaatgtc attatcattg ccgccaccaa ccgccccgat attttggacc cagctttgct
    15361 gcgccccggc cgttttgacc gccgtgtgac tctcgaccgc cccgacgtga agggacgcga
    15421 agccattctc aaagtgcatg tcaaaggcaa gccgttggat ccgaaggccg accttgcttc
    15481 ggttgctcgc ggtacacctg gtttcgtggg tgccgatctt gaaaaccttg tcaatgaagg
    15541 cgccattttg gctgctcgcc gtaataagaa atccatcggt caatctgaat tggaagaagc
    15601 cattgaacgc gtggtgatgg gacctgaacg caagtcccgc ctgatctctg atgaagaaaa
    15661 gcggatcatt gcctaccatg aagctggtca tgccatcgtg gcaaatgcca tcgctgaagc
    15721 agaccccgtc caaaaagtga ccatcgttgg gcgcggacag gctggcggcg tgacctggtt
    15781 ccgccctgat gaagaccgca ttctcatgtc tcgcaagaag atgctcgcca ctttagccat
    15841 ggccctcggt ggtcgtgctg cagaagagct tatctttgac gatatcacct ctggtgcatc
    15901 caatgacatt gagcaggtga cccgcatggc acgtgccatg gtcacccgcc tcggcatgag
    15961 tgccgaaatg ggcaccatga cctacggcca aaaagaggaa atgatcttcc ttggccgcga
    16021 aatttccgaa caacgcgatt attccgaagc cgtggctgaa cagatcgatc gtgaggttcg
    16081 caagatcgtg gaagaatcat ataagattgc cagaaacacg gttaagaaat atcgcaaaca
    16141 gcttgatctt gtggccaaac agctcctcga agttgaatcc attacccgtg aagatttcga
    16201 aaagatattc cccgcgcccc tcggcaagaa gagcggcaca ccgcaagcgg tataaattct
    16261 tgtaaagaag caaccgataa aaaagaggct ttccaaaagg aaggcctctt tttattacca
    16321 attaccaatt accaattact tactttccac cctctttgtg ctgcctcacc aactcacggc
    16381 gcaaaatctt gccaaccgtt gtctttggca gttcagtgcg gaattcatag tgggtgggaa
    16441 ccttatacgg agcgaggtgt tccttgcaga aggctttgag ttcggcttct gtcaaggtct
    16501 caccgggttt caccaccacc caggctttca ctgtctctcc gcgctgtggg tcaggaatgc
    16561 cgcccacgcc tacttccaac accttcgggt gatccatgat cgcttcttcc acttcacgcg
    16621 gccatacctg gaagccgccc ggcttgatga gttccttctt gcggtcaaca atgtagaagt
    16681 aaccatcttc gtccatgcgg gcgatatcgc ctgtgaaaag ccaggtcttg ccatctttca
    16741 tctgacgcag gctgttggcg gtttcggtgg gcatgttgtg gtagcccttc atcacctgcg
    16801 gcccgttgac gatgatctcg ccgatctcac cctgcggcaa ttcggtttcg ccatcatcaa
    16861 ggctgatcag tttgatatcc acatctggca gcggcatacc gatggagccg gtcttgttca
    16921 cgccattgag cggattgcaa tgcgtggcgg ttggcgcttc agagaggccg tatccttcga
    16981 ataccttgcc gcccgtcagt ctttcaaatt catcttttgt ttcgcgcatc aacggagccg
    17041 accccgaaat acaggctttg atggaggaaa ggttgtattt ccctgccttc acatcgggat
    17101 gattattgat gccgttgtac agcaaaggca caccggggaa gatggtggcc ttgtatttgc
    17161 tgatgttttc aagtacgtcc ttcagatcgc gtgcattcgg caccatgacc atactggcgc
    17221 catttgccat ggcaaaattc atccccgcga ccatgccata cacatggaaa agcggaatgc
    17281 ccatcaaaac cacctctttg ccaggttcaa gagcgggcat ccatgatttg atctggaggg
    17341 tattggcaac cacattgcgg tgcattgcca cagcaccctt tggcacaccc gtcgtaccgc
    17401 ccgaatactg gaagagggcg gtgtcatccg gagttacttc tacgttgggt ttcggggagt
    17461 tcgcatgttt ggcaagcagg tctttcatcc acacatcccc gctggccagg gtatccacac
    17521 ggtcgccttc ctttttctct ttgacaagcg tgaaaagcag acgtgtgatc ggaggcaggg
    17581 tttccttgat attcgtaaca atgatcttct tgagtttgga tcgttcacgt gcagtcttca
    17641 acttatcgta aaaacggctg agtgtgaaca tgatttcaat attggcatcg ttcacagggg
    17701 tgatgatctc atccacaggg taggtggggt ttaccgccac caccgcacca cctgctttta
    17761 aaatgccgta atacgcaatg acaaactgcg gcgtatttgg catgaagata cccacccgat
    17821 cccctttttt cactcccatt tcaaccagtg cggcagccat gtggtcggtc aatgcattca
    17881 tttcacgata cgaaatgacc gcacccttga agatcgtgca ggcgcggtca ggatattggc
    17941 gcgcggcttc ctctaaaaaa tggaaaagcg gcgctttagg gtaatcaatt gtttgtggta
    18001 cacctttatc gtaattcgcg agccacggac ggttgttcat gatttgctcc tccttcaagg
    18061 gatgtgtgaa gtatatacaa tcaagaatca aaagtcaatc ggttgtggat atgtttttca
    18121 acttccatca ccacgccctc tttgactctg aaccacgcga tttctggatc agactccttg
    18181 aaccaatttg cctgcctgcg gacgaaaacc cgcgtcgctc gcttcatctc aactttggcc
    18241 tgctcggtcg tcaattgacc ctgcaccacg ctgacacatt ctcgataccc gatcgccgac
    18301 atgcttggca acgatggggc gtaccctctt tccagcagac tctgcacctc gtccacgaac
    18361 ccagccgaaa acatggcttc gatgcgctca tccacccgtt gataaagctc ctcgcgtgga
    18421 cgagtcaacc cgatggtaat taatcggtag ggcgcttccc cctgtccgcg ttgttcggag
    18481 aatttcctgc cagtagtcaa aatcacttcc agggcacgga tcgtgcggcg tacgttgcgg
    18541 gcatcaattt tgagtgcgga ttctgggtca agataggcta gtttagcgtg tagccactct
    18601 gcccctcgtt cttccttcgt cttttccaac tcatcccgca tcttcggatt ttcaaccacc
    18661 tctggcggct gccatccctg cgtgaccgcc cgcacatatt gccccgtgcc accgactaaa
    18721 aaggggagcc gtccgcgtgt gtggatctct gtaatgattc tcttggcttc ctgctgaaag
    18781 actgccaaac taagagtctc atcgggttca acgatgtcaa tgagatgatg cggaatgcga
    18841 ttcatttcct cctgcgaagg tttggctgtg ccaatgttca tgccgcgata aaagaggcgc
    18901 gaatctgccg agacgatctc gccgttcaat gattccgcga gttggatcgc aagttctgtt
    18961 ttaccgaccg cggtcgggcc aacgatgagg atgagaggtg gtttgttcag tgacattgtt
    19021 atgaattgat tggaaaaatc atggggcttt ctggtacact tttataaagt tgatttggtt
    19081 cccgagacat atcgggacaa agtgaatgtt gaatagtttc tattttatct cttgttgaac
    19141 aaatcacgag accaaaaggt gagcaaatga ttaactggca ggaattaacc attgatatgt
    19201 tcgtcgagca attgcggacg gtttatcaac gcacatatgg tgacatcaac agtgattttg
    19261 gccgaatcgt tgcctggtgc ggacgccttg cgttggagaa cattgccaat tccgatgcgc
    19321 tctatcacga cattgaccat accatcatgg ttgcattggc tgggcaggcc attgtggagg
    19381 ggaaacatct gcgcgaaggc ggggtgaccc cgcgtgattg gatgcacttc atgatcgctg
    19441 tgctttgtca cgatatcggc tatgtgaagg gagtgtgcaa taacgacacc aaggaccaat
    19501 tcaccactgg ggtggggaac aagatggtct tcatttctcc cgaaggcact gatgttgccc
    19561 tgaccccata tcacgtggac cgcagcaagc agttcgtcat cgaacgtttt gggacggggc
    19621 tgcttcagga tatgaccaaa ttcctcgatg cggaattgat cgcctcctac atcgaaatga
    19681 cccgcttccc atcccctcca ggtgaaatgt acaaggacac aaaaggcttg ggaggccttg
    19741 tccgtgcggc ggatttcatt ggtcaactcg gtgaccctga ttacctgcga aaatcccccg
    19801 cgttgtttta cgaatttcag gaattgggcg tcaacgaaaa attcggctac aaatcaccca
    19861 acgatttgcg caagaattac gcatcttttt actggaacag tgtcagcccc tacattcaag
    19921 atgccctgca ctatctccgc ctcaccgaag atggcaaaca atggatcgcc aacatgcatt
    19981 cccacgtctt cgatgccgag catgaccgcg gggcgcggta attatccaaa taagagcgat
    20041 acacatttac aaaaaataaa aggatacgtt ttaggcgtat cctttttgaa ttaacttggt
    20101 gaggcaatgc acgttgccat ttgggctatg caggcttcga atactcccga atatcatccg
    20161 tcgcttcgtc cagctttgcc acttcttcat cggtcatgcg ccagcctgcg ccgcccgcat
    20221 tgctttcagc ttgttgcagg ctcttcgccc cggggatggg aagtgcgccc ttgcagatca
    20281 gccaattcaa agccacctgt gaaatggttt tatcgccatg attcaaaccg atctcctgta
    20341 acaacttgag tacaggcggg agtttcttga tcaattccgc gtactgtgag gcgcgcacac
    20401 cgggcggcgg attttctgat gaatatttcc cggtcaataa acctttttca agcggggaat
    20461 aggcgatcaa gcggattccc aattctttac agcgggcgag tgtgccattc ttttccacct
    20521 cgcggctcaa caggctgtaa tgcacctgat tcgacgccag cggaattcca tgccgcgcca
    20581 gtgtggtgta ggcgcggatc attctctttt caccgaaatt ggaaacgccc actgtgcggg
    20641 tcaacttgcg ctgaacacat tcgaccatgc cttccatcat cgtttcagga gtcatcaaag
    20701 gcgaaggcca gtggatctgg tacaggtcca ccgattccag tccgaggcgt tccaggctgc
    20761 ctttcaaagc gcggggaatg aatcccttgg taagccgcca gggcatggga aagaattttg
    20821 tggcaaccag cacgggctgg tctgtttctt tcaagaactg tccgagcagc cgctctgaat
    20881 atcccgatcc ataaatctcg gcagtgtcaa cgaagcgaat tccgagtgcc agcgaggcat
    20941 cgaacgattc gcggatttcc ttgtcggtgt gtgtctgccc atagccccag acgattcggt
    21001 caccccactg ccacgcgccc aaccccattt cgacggcgtg caggaacctg gtttcattgc
    21061 tgatttcggt cacgatgcct cctgtgaaga acagccccga ttttatctta aaaaaccccg
    21121 ttcaaaaaaa atacaggtta caattcagcc cgcttaaaaa attttagtgg aactgatgga
    21181 atgcgagtct ctgacctccc gccttgggat ggagcggggg ctggcgtccc ctgagttcca
    21241 ggaggaatct tggaatgccc aacttgaatg cgcgggaagt tgaatccgca tctgacctca
    21301 gccgacggct ggggctgccc ttttcaaatt tagccttgct gacacgggcc ctgacccatc
    21361 gctccttcgt caatgaaaat ccgatggcag tggaagataa cgaacgcctt gaatttcttg
    21421 gcgatgccgt gctcgatttc attgtaggag cctgggtgta caaccgtttt cccgaaatgc
    21481 ccgaaggcga cctgaccaag gtgcgttcgg cattggttcg caatgaacag ctggcgaagt
    21541 tcgcccgcaa attggaactg gggaatgcct tgcggcttgg ccgtggtgag gcggcttccg
    21601 gcggctccaa ccgcgacaat ttgcttgggt ccgctttcga ggcgctggtc ggcgcattgt
    21661 ttttagaatc aggtctcggc ggggtggaag cgttcgttca tcccattttg gaagatgccc
    21721 gcgaactcat cctgaacgag attcacgacc ccaaaagcaa attgcaagag tgggcacagg
    21781 cgcagaaatt gggcgcgccc cggtatcggg tcatcggcac aagcggacct gaccatttgg
    21841 tcacatttga gatcgcagtg gaaattgcag gcgcggaaag agggcgcggc acgggcacca
    21901 gcaaaagcca tgccgaacac gccgccgcgc aggatgcgtt gaatcagttg aatgtgctgt
    21961 aaaaatgttg actggtttgc aggttgacct ttcaaatgta aacttgaaaa atccaaaatc
    22021 tgaaatcgta aatcgaaaat aaaacatgcc tcttcgtcta aaatcgcttg aactgcaagg
    22081 atacaaaact ttcgcatcgc ggaccgtgtt tgagttcgcg agcggaatca ctgccatcgt
    22141 cgggccgaat ggttcgggga agtcgaacat agccgactcg ctgcgctggg tgttgggcga
    22201 gcaatcgtac acactgctgc gcggcaagaa aaccgaagat atgatcttct cggggtcgga
    22261 acatcgcgca cgcgcaggca tggcgcaggc cacgatcact ttcgataaca ccgagcaatg
    22321 gctgcccgtg gatttttcgg aggtggcact ttcacgtgcc gcacatcgtg atgggcacaa
    22381 cgattatttg atcaacggcc agcatgtgcg cttgcgtgag atgaatgaat tgctggcgca
    22441 ggcaggtttg agcgaacgga cttataccat cctcgggcag gggttggtgg atgcttcact
    22501 ggctttgaaa gccgatgatc gccgccgcct gtttgaagaa gcggcaggtg tggggttgta
    22561 ccgcgcccgc cgtgatgaag ccctgaaacg ccttgaaaat accgagcgta atcttgaacg
    22621 tgtacttgat atcatggccg agcttgagcc gcgactccgc agtttggagc ggcaggccaa
    22681 acgcgccatt gattttgcgc gcgcgcaggc agatatgaag ttgattttgc gcgagtggta
    22741 cggtttccac tggcaccgcg ctcaacgtga cctgaccgat gcacgcgaaa ccgtgcgcgg
    22801 acaggaagta cgcgtgcgtg acctgcgtgc cgttcacgag aaagcacagg ccgaatacac
    22861 ctccttccgc gaaagacttt cgggtctgcg tgctcaattg aatggctggc atcgccaatc
    22921 ggcagatttg cacaaccagc gtgaagccat cagccgcgaa ttggcagttc tcgaagaacg
    22981 ccgccgcgca ctgaccaata cccaggcctc catcctttcg gatcaggaac atgcgatgga
    23041 tgaactgcac cttgccaatg accgttttgg cgaagcggag caggaactcc tccgtgtgca
    23101 gggcgagtat gaagaggcca aacgtcagct tgaaacctgc aaccttcaac tcgcaaatcg
    23161 aaacgcggag cgagccacgt tggaggaaca ggctcaactt ttacgcaacc agatcgaagc
    23221 ctttagccag caacgtgccg agaacaacgc acgtctggat gaattgaaat cccgcctcga
    23281 agcgcaatca cagcgcattg aacaaaccaa acaggccatc accagcggcg agtcgctggc
    23341 tgcgaaggcc agatcgcaat atgaatcggc gcacaaagcc cgcgaacagg cggcattggc
    23401 attgcaggaa gccgaagaaa aagtcatcag cagacgcaat gaagtggaac gcctggaacg
    23461 tgagcgccgc gatgcgctcg aaaaacgaac catcgaagaa tcggatcatt cgcgtttgaa
    23521 ggcacagctt gaggtgttgc agcaatcgga gcaatcgctg gctggttatg ccgaaggcgc
    23581 tcgttatttg cttgatgccg cccgccgcga tccacagcgt ctgacagggg tgcggggtgc
    23641 gctcagcgcc gcgttggatg tccctgccga gcttgaaacc gccatcgccg ccgctctggg
    23701 tgatacgctc gatgcagtct tgctggatga aaatcaactc gaagaagcgt tgggtctgct
    23761 tgaatccgat gaggcaggcc gtgcggcttt gctgccgctt gtaatgcaag aaaatatctt
    23821 gcaccaccct tccgatgaaa attgccttgg cattgcctcc gaacttgtca acgcgccaga
    23881 ggaaatgcgc ggtgccgtac ggttggcgct gggccagacc ttgatcgtcc gaaaccgaaa
    23941 taccgcccgc attctggtga aggatttgcc gactcacgcg cgggttgtga cactgcgtgg
    24001 agaggtcttc cgcggtgacg gtttgatcat cgctggaaaa accgcttcca gttcgaccct
    24061 tggcaggtca cgccaaaaac gggaatttga atccgccttg agcggactcg tcgtccgttt
    24121 ggctgaatcc aatgaccttg tggagcgttt gtcgaatcaa ctggcgggcg cacagcgtga
    24181 gttggcacag gccgataccg atgcgcgcga atcccgcgtc agactcggtg aaattcaaga
    24241 gaccgagcag caggccgggt tggagtctga atcgacgagt cgtcagcttg aatggcagaa
    24301 aaagcaattg gaacagttgg aagccgaggc ttgggaatct gcttcgaccc agcaaaagat
    24361 cactgaatct caggctgagg ttgaacagcg ttcgaccgag acgctttcac aattaaagaa
    24421 tatcaatgca cggttgaatg aaatggccgt ggatgaatta caagatcagg tttcgtattg
    24481 gggcacacgt gtggcggttg ccgagcaaag tgttggcagc gccgccgcca aacgcgatga
    24541 gcgcaccaat gagatcacgc ggctcgattc acggcgcgtg gaattgatct ctcgtttgca
    24601 ggaagccgaa ggcttgctca ataatctgga taatgaaaag gcgcgtttac gtgagaacga
    24661 gtcgggtctg cacgggcaga ttgacgagat gcgggttttg attgagcccg ccgaaaaaga
    24721 tctggaaatg gccgagcagg aggaagcccg tctgcaggaa gccgaggccg ccgctcagcg
    24781 tgcttttgcc aacgccgaac gctcatatgg gcaggtgcag ttggaacagg tgcgcaagca
    24841 ggaggccctc gataatttgc accagaagat caccgatgat tttggcctgg tgatgttcga
    24901 ttatgcggcg gatgtttctg gccccgtgcc gcttccgctg gacggcatgg tggagcaatt
    24961 gcctgttgta accgaactcg cgcccgagat cgaagagcag ttgacccatc accgcgccca
    25021 gttgcgccgc atgggtccca tcaatcctga tgcgaagact gagtacgatc aggaaagcga
    25081 acgctacacc ttcatgaagg cacaggtgga agacctgcgc aaagccgaag ctgacttgaa
    25141 gcaagtggtc gctgaactgg atgaaatcac caaaaatgaa tttgtgcgca ccttcgatgc
    25201 cgtggataag caattccgcg aaacctttgt gcgtttgttc ggcggtggtt ctgcacgttt
    25261 ggctttgacc gaccccgaaa acctggtgga tacgggtatc gaaattgagg cgcgtctgcc
    25321 tggccgccgt gagcaaggct tggctttgct ttcaggtgga gagcgcagtc tcaccgccat
    25381 tgcgttggtc tttgccttgc tcaaggtttc gccgaccccc gtctgtgtga tggatgaagt
    25441 ggatgcgatg cttgatgaag ccaacgtggg tcgtttccgt gacctgttga tcgacctgag
    25501 caaggataca cagttcatcg tcattaccca taaccgtaat acggttcaag cagctgatgt
    25561 catttacggt gtgaccatgg gccgcgactc agccagccag atcatcagtc tgcgcctcga
    25621 tcaggttacg gatgatatgt tgaagcgggg gtaaacaaaa ggatgaaaga taaaggatga
    25681 aggatgaaaa agcccgcctc attttgaggc gggctttttt gtatcagttt tttgaaacgc
    25741 atatttgctc gtttttactg cgaagaagcg gtaagtttta tggcttttct ttgcaagctt
    25801 tgcgattcaa atttgttaat tacggggcag cggttgcatc cacagcggat ttggtctcag
    25861 ctaccgcggt gtcttgagcg ttggcggctt cagtggcgat ggtggagaaa ttcggttcgc
    25921 tgggaacacg ttcctgccag aagtcgaaga tttccacgcc gtattcttcg cgggcagcgg
    25981 tcagccattc ctggaagacc ttgtcacggg cggcgttgta ctgatctgcg gtgagcgggc
    26041 gttcctgctt cgcgatcaat tggatgatgt ggaaaccata ctggctttga acaggggtgg
    26101 tggtgaaatc gccggggttt tcgagggcga aagcggcggt ttcaaattca ggaaccatgg
    26161 cgcctgtgcc gaaccagcca aggtcaccgc ccaaagcacc ggagccggtg tctgtggaaa
    26221 attcctgggc cagggcagcg aaatcttcgc ctgctttgat cttttcgatg agattgagcg
    26281 cggtggcttc atcttcaacg agaatgtggc gggcccaaac ctgctcttcc gtgtgggcta
    26341 cgtcagcggt gatgatgtcc ttgagtttgg tttcgaagag ctggttttcg aagaccagtt
    26401 tgtacacatt gtcgtcgaag cccagttcca tcaggcgctc gttggcgtca ttgaactcgg
    26461 tctggaaacc ttctgccgtg tagggggtgg ctgtcggctc cggagtgttg gtcggaccaa
    26521 gtgtcggggt gacagtgagc gtggcggtgg gggtgaaggt ggcggttggg acaagagtcg
    26581 ctgtggcttc cggggtgccg gtgatcgtcg cagggtcaat ggttgcggcg ggggcactgg
    26641 tggcggtgcc agtcggctct gaggtgtttt ccggcgttgc gctgggcagc ggggtgatgg
    26701 tcacgatatc gaaggcgcca gcaggcggtt cgggcatgat cacttcagta ggagtgacag
    26761 atggagtggg cgacccgttc gggaaatatc cgaacgcgcc ttctgtcaac tcactgattt
    26821 cctcttcact caccgtgatt ccgcgtttgg cggcttcctg gcgaatgatc tcttcattga
    26881 tcatctgatc aagcaccgct cgaccgatgg aggtggggga atccagagaa gcctggatct
    26941 gctgtaactg tgagcttaca tccagaccga actgcgcata ctgtgagtag gtctggtatt
    27001 gggccagtaa ttgctggcgt tgtaaacgaa cgcgcgcttc aaactgtttt gcaaggatct
    27061 ctgtttcacc aaccttggcg accgggcgct tgagctggaa ataattaata tccaaatagc
    27121 cgtacaaaac caaaagcaga accgcggcca ggatcccaat aaaggtatat aaaatgatct
    27181 ggctttgctg cttttcacgt tgaaggcgtg caacgtgttt tttatgcaaa acgggattct
    27241 cgttaggcat cttcttgctc ctttatgatg gttgcatatt gcttttcaat gggtgtcact
    27301 cgcagattca tcctaccgcc ctgagggcga catgcctgac ctcgcccggt cattccattt
    27361 cggtttgtat tattcgggga agggtgggcg ctgaaaatct ccggcgcaat gacaacccac
    27421 ggctaatcac acctgctgtg cgatcagcaa agggtcggca taaattcggg gcatgggaat
    27481 cgctcccata ccccgcgaaa ttcttaaaaa actatgaacg gttgtcgtcc agggcgcggc
    27541 cgctgaaggg caaaaatgcc acgtggcggg cctgcttgac ggcggcagcc acaacgcgct
    27601 ggtgcttggc gcaggcgcca gtctggcggc gcgggcggat cttgccttct tcggttatgt
    27661 atttgcgcag cagatcggtc ttcttgtaat caatgaccag catcttgtcg gcgcaaaatt
    27721 ggcagaactt gggcttggcg aaaaatctgc ggtcaccgcc gccttcgccg ccgcccgaat
    27781 agttgcgttc ttcactcatg atttactcca aacaaactaa aagggaaatt catcctccgc
    27841 catggaagag ccattgtcgg cggaagaaag atcggcatcc tgtgaatggt tgttgtttgc
    27901 atcgcggcga tcaccaagca tcatcatctc gttcgcaacg atctccacgc tggtatgctt
    27961 ttgaccttcc ttgtcatccc agcggcgggt ttgcaaacga ccctcaacaa aaacctgctg
    28021 tccttttaca agatactgtt tgcagatttc agcaaggttg ccccaggcca ccacgttaaa
    28081 ccactcggtc tcactgtggc gctccccgtc cgcgctgttc cacgaacggc tcacagccac
    28141 cgaaaaagtg gtgacgggtt ttccagatgg tgtataacgc atctcagggt ctttcccaag
    28201 atgtccgatg atctgaactt tattaaggcc tcggctcatg ataattctcc ttgataaaat
    28261 taccgggcaa tgcaatatgg aaactaacca acaacagaaa gcatgtgacg aatgatcgtc
    28321 tcttggtagc gcaggttgcg ctcaagatca gaagtcgcag atggattcat agaaacgttc
    28381 aaaagaacga actgaccttc gcggtgcttc ttgatgatgt aggccagctt gcggcggccc
    28441 caaacatcaa ccttatcaac actgccgcct gagtcactga tccaaccctt caccttatcg
    28501 agcacgccgt taaaagcatt ctcgtccagg tctggctgca tcacacagac taattcataa
    28561 ttacgcatag ggaaaacctc ctttccatgg gtttgttcct gttcaaaccg ttggttagcc
    28621 aggaacggaa aggcacgtat ttggatgacg tacctaattt agcgggcgaa gtttaccaca
    28681 aatttaccgc tttggatgaa accccgcttc gcacctaact gaaaactgct cccgtccgaa
    28741 aggcattttc agcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntacct
    28861 cacatagatc ggattactgt agatccaccc acgcagtttg cccaaatacc tgcgatacac
    28921 ctcaattcga tagacccctg catcagtagt gatatgggtg caggacgatt tattcttcca
    28981 cgtttgaatc acaaccccgt ccttcaacaa ccgaatctct gcatctgacg gcagtttagc
    29041 ctgcaaggtg acacctccct tggcgggaat ctcatcgccc atgatcgccg ttttctcaag
    29101 tccctgcgcc gtaaagcgga atccgcgggt gggggcgggc atgtcatacc ctacaaagca
    29161 gtgacctgcc gccatcgcct cgtagatcag ttttttatcg ttgttcacat cgccgctcaa
    29221 gccctcgggg aggatcaagt gcgtgttgac cgtacggaag tgaaactcat aggggaagat
    29281 cacgcggctg atcggtccca tgctcatccg caaagcatgg gcgtctgaac cgccgatggc
    29341 aaccacgcgt gcccctgttg ccagtaattc gtcccatttc aagatcgtat tggggatggg
    29401 ttggtgcgct acgaaaaaag ggaaaaaagc ataaaatgca ccatgtaatt ttgtgggaac
    29461 tacggtcttc aactcgctta acgcattcca aagttcgatg ccagtgtaat tccgtaccga
    29521 ccaatcttcc caggaaatat ccgtctcctt aaaagcgggt gcggcagggt catctgggtg
    29581 ggcgatgaag gccagcccgc cagaagcctg cacctggtca atgagcagct gcgggtcatg
    29641 ggcgaacgta gcgagttctc ttccgctgcc aaagaccagc aggtgatttt tctgcgggtc
    29701 ccgcgcctga tcgtgaactt cctcaccgat caacatgagg atctttttat ttttttcctt
    29761 gtaatagcct tcaaaaccct gaaccaatac attgtgatcg gttacgatca cggcatccac
    29821 acctgccgca agagcagctt tggcgatgtc ctgatgcgtt ccgcttccat cagagtagcg
    29881 tgtatgcata tgcaaattga caatgacttc agacatacat tcctctatat caaattcttt
    29941 cctcttcctt cattaaatac gtaggaagag gaaagacaat tcaagaaaac ctttgttgac
    30001 gattcacttc tgaaacaggt ataatctgcc cgctaaattt aaggaggcac atcgtatgga
    30061 aaagtttttg gatattgcgc tgattataac ctcagtgggt ttgatccttg gcgtcatcct
    30121 gcagagcaag ggcgcagggt tgggcggctt gaccggcgct gataccggtg gcgtatttac
    30181 cgcccgccgt ggcatcgagc gcatcctctt ttgggtgaca atcggattgg ctgtgatatt
    30241 ttttggtctc gtcttcgcag tgatccttat tggaggctaa aaaccgcaag cctttgaagg
    30301 gccgccgggc cattgccccc gtgtggggcg gcggcggcct ttcttctttt tacaacaaat
    30361 tatgaaaaaa ctacgctggc agattctggt tgtagcggtc acggtggtga tcgtcgcttt
    30421 gctcttgctc agccaacaac caattagcac gtccttcttg cccgaagccg caccgggggg
    30481 aatctatacc gaggcgctgg tgggctccat gggacgtctc aatccaatgc tggattggaa
    30541 caaccctgct gaccgggata tcaaccgcct gatctttagc gggttgatcc gttttgactc
    30601 gcatgggctg ccccagcctg atctggctga agcatggggc acctctcccg atggcacact
    30661 ctacaatttc tcgatccgct cgaatgcggt ctggcatgat ggtcaacccg tcaccagtga
    30721 tgatgtcatc ttcaccattg agatgatcaa aagcagcggc tcgctcttcc cacaggatat
    30781 caaagacctg tgggcgcaga tcgagatcaa gcggtttgac gataaaacct tccagttcaa
    30841 acttcctgaa ccgtttgcac catttttgga ttatgtaaca tttggcgttc tgccaaaaca
    30901 cctactcgaa actgttcccg cagaccagct tgccacagct caatttaact tgcagcccat
    30961 tggcaccggc ccgtataagt ttgagcgcct gttgaccagc ggcggacaga tcgtgggcgt
    31021 tgcattggcc gtgaacaagg aatattatct gccaagtgct ttcattgagc agatcgtctt
    31081 ccgcttttac cctgactcag cttcagcact tgatgcctac cagcaagggg atgtccttgg
    31141 ggtcagtcag atcaccaatg atgtgctgca gcaagctctg atggagccta cactctccgt
    31201 atacaccagc cgccttccgc aattgggact ggtctttctt aatctaaaca atcccaccgt
    31261 ggcctttttg caaagcgaca aggtccgaca tgcgttgttg cttggcacca atcgcgagtt
    31321 gatcgtttcg cgcatcatgc agggacaagc catcgttgca gatggtccga tcctgccggg
    31381 gtcatgggct cattatgatg agatcgagca ttttgagtac gaccccgatg ctgctgcggc
    31441 gctgttaaaa gctgaagggt atgttatccc agccagcggt ggagatgtac gcgccaaaga
    31501 cggtcagtac ctgaacttta cgctgaccca tcccgatgac gagatccaca cccgtatcgc
    31561 gcaagccatc caatcggatt gggctttgat cggtgtacgg ctggatttgc agccagtctc
    31621 ctatgactcg cttgtcaatg attatttgac cacgcgaaat tatcaggcgg ctttggctga
    31681 tctgaataca gcccgcacac cagacccaga cccatatttg ttctggcatc aatccgaggc
    31741 cactggcggg caaaactatt cccaatggga taaccgtacc gccagcgaat ttttggaatc
    31801 agcccgtaca gaagcagatt tctcttcaag agcacggttg tatcgcaact ttcaggttgt
    31861 gtttaccaaa gatatgccat cactgccttt gtattacccc gtctattcct acggtgtgga
    31921 tgcacaggta cagggtgtgc aggttgcacc gatgtatgat gtcagcgacc gccttgcttt
    31981 ggttgccgaa tggtatcttg taacccgccg tgccctcgaa gaacaaaccc cggtgccgac
    32041 tgcggcacca tagaaaatga ctgaagtctt ttctatcttg aaaaggagtg acaatgtctg
    32101 acgcaaaaaa ggcaatcgaa tacgcccatc agaatcgcga gcgtttcacc cgcgagttga
    32161 ccgaattcat caaaatcgaa tcgatctcca ccgatgatgc ctacaaagcc caggtgctgc
    32221 aggctgctga gtgggcggct ggccatctgc gcagtatcgg gatcgagaat gtaaagctca
    32281 tgcccaccgg cgggcacccc atcgtgtatg gcgattggat caagaacccc ggcgcgccga
    32341 ccgttttgat ctacggtcat tacgacgttc aaccggttga tcccatcgaa ctttgggaga
    32401 cctctccatt cgaagcgacc gtgaaaggtg attatctttt cgggcgtggt acttccgata
    32461 tgaagggaca ggtaatggcg actttgaatg ccgttgaagc catcatgaag acgggcgatt
    32521 tccccgtcaa cctcaagttc atgctggaag gggaggagga aattggctca atgaatatgg
    32581 gcaatttcct gccgcaacat aaagatttgt tcaaggccga tttttgtttg aaccccgatg
    32641 cgggtgcgat ctctgctgat gagccgacca tcactttcgg cctgcgcgga ctcgcgtatt
    32701 ttgaagtcca cgtgtatggg ccgacgcgtg atttgcattc aggtttgttc ggcggcacgg
    32761 ttcataaccc tgcacaggca ttggccgaat tgatcgcagg gatgcacgat gccaacggca
    32821 cgattacatt gccaggattt tacgattcgg tccgccctgt taccgatcag gaacgtgaag
    32881 ccacagccaa gctgcccacc acaccggaaa gttatttgca gcaaactgaa gtgccagcct
    32941 tgtggggcga gaaggaatac acgcctgcct tgcgcaccgg tgcgcgcccc acgctggaag
    33001 tgaacggtct gctctcaggg tggactggtc ccggctcgaa aacagtgtta cctgcgaagg
    33061 caatggctaa gatctcctgc cgccttgtgc ccgatcaaga ccctgctgaa gttcacaagc
    33121 agcttgtccg ctacatggaa gaacatgccc caaagaccat tacttgggaa gtgaagatcc
    33181 tgcacgatag tccctgggca attactgacg ttaataacaa aggcgtccgc gccatggcag
    33241 ctgcaatgga aaccgtgtgg ggcaggcctc ccttgtatcg ccgtgaaggc ggctcaattg
    33301 gtgtggttgc catgctgcaa aatatctgcg gagttgaatc ggggcttgtc ggcggcggcc
    33361 tgcccgatga caatattcac tccccgaacg agcgtatgca tttgccaacc tggcacaaga
    33421 acatggatgc attcatccat ttcttcttca atatgaaata gaaataaaaa attgacagtc
    33481 acccccaggg tgactgtcaa ttcataagga ttatttatgg aagacagaat tatcacctat
    33541 ggcggacagg ccgttctcga aggcgtttta atgcgtggac aaaaagcatt tgccattgcc
    33601 atgcgcgctc ctgatggctc catcaccatt cataccgaaa aactcgcaaa agtgtaccgc
    33661 tcagggatca ccaaagtccc gttcattcgc ggggtcattt tgctgtggga tgccctcgga
    33721 ttgggcatga aagccctcac catttccgcg aatacccaaa caggtgagga tgaaaaactg
    33781 gaaggccccg ctctttatct gaccctggct ctgtccatga ccattggcat tggtctcttc
    33841 tttttgcttc ccgctggcat cggcggcgca gcggaacatt atctcggctg gagttcctgg
    33901 ttgaacaacc tgaccgaggg tcttgtgcgc ttagggttgc tggttggcta tgtttgggcg
    33961 atcagcttca tgcctgatgt aaagcggctc ttcatgtatc atggtgcgga acacaaaacc
    34021 atcaacgcct atgaagcagg cgcggaattg acccccgaag ttgttgcaca ataccccatt
    34081 gagcatgccc gttgcggtac agcttttctg cttacattgg ttttgctatc cattttggtt
    34141 ttcaccttgc ttggcccatt gcctttgctt tggcggcttg cttcccgtat tttgttcatt
    34201 cccatccttg caggcatcgc catcgaatac atccgctgga cggcgaatca tttggacagc
    34261 cccttcgttc agtttttgat caagcccaat ctcgctctgc agcacctcac cacccgcacc
    34321 cccgaccttg ccatgctcga agtggccatt cagtcatttc agtccatgcg caaagcggag
    34381 agtgaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca atgcggatcg
    34501 gggtgccgag gaagctgtgc tccgcacgga tctggttttc aagatagcgg atgtaagtga
    34561 agtgcatcag cttcggctcg ttgacataga tcaggaaggt cggcgggtcg gagcggactt
    34621 gtgtaccgta gtacatcttg agggcgcggc ctgcatgagt tggatgcggg tgcgcgtcct
    34681 gggctttgtg gatgatcccg ttaatggtgg atgtggtcag acgggcaagg cgttcctctt
    34741 gcacttgcaa tgccaacggg agaacctgct ccattcgctg accagttttc gcggagatga
    34801 agagaatcgg tacgtaatcc atgaagttca attcgtggcg aattttgatc gtgtactctt
    34861 ccatcgtctc gttatttttt tcgatggcgt cccatttgtt gacgagcacc acacatgatt
    34921 tccattcatc gaggatgtaa cctgcgatgt gggtatcctg cgcggtgatg cctgtggtgg
    34981 cgtcaatcat cagcaaggcc acgtcagcgc gttcaatgga cttgaaggcg cgcagcacgc
    35041 tgtactgctc cacgccgtgt tcaatcctgc cgcgacggcg aatgcctgcg gtgtcgatca
    35101 aggtaatggg caggccttca tattccattt tggtgtcaat cgaatcacgg gtggtgcccg
    35161 cgatcgggct gacgatgact cggtctttgc cggtcagttt attgagcagg ctggatttgc
    35221 ccgcattcgg tttgccgaca atggcgatct tgatgctgtc atcttcttct tcattttcgg
    35281 gcgccttgaa agatgatacg agtgcatcga gcaggtcgcc tgtacttgtg ccgtgaatgg
    35341 ctgaaatggc aaagggatcg cccagcccga attcgtaaaa ttcactggcc gagtcgcgcc
    35401 tttcctgtga ttcgcatttg ttgaccacca aaaaaatcgg cggccagaag gtgccatcgg
    35461 gtaatttctt ttgcgagcgg cgcaggatgt tcgccacttc gcggtcgggt tcggtgacgc
    35521 ctgtttcacc atcgttgatg aagaggactg catcggcttc ctgaatggca acctgggcct
    35581 gcctgcggat atcttcaatg aattcgaccg agccgaccga aagcggagtc tttccgccgt
    35641 gggttgggtc gataccgcct gtatccacaa tgctgaaggt gcggccattc cattccgctt
    35701 caccaaataa acggtcacgg gtggtaccgg gggtgtcatc cacaatggct aaacgttcgc
    35761 cagcgaggcg gttgaaaagg gttgatttac cgacattggg tcggccgacg agtgctacga
    35821 caggtttcat ttttattttc caattttaag gtgtcggcgt taccacaggt gtgccgatca
    35881 gagagatcac aacttcaagt gtgttgggca ggatcgattc tacaacaata tctgagatca
    35941 aaacttcgac tttcggaatg acctgatgcg taccgattcc gagccccgtc aggtcaacgg
    36001 tcactcttac cccttgcctg gtcaatgtgt cgaggagcgg gagcggcccc gaaagaatga
    36061 catccaccgt catgggtgag acttgtgccg tcagcccggg gtcaatgcca atgatctcca
    36121 ctttttcact gccaagcgta acactgcttt caatgggtga gatgcccgct tgaatcaaaa
    36181 cggtttgctc gccaacaatg gagatgccct cgggcagatt caatgcgagg cgggtgttga
    36241 tgttttcctg cgcattctgc aaactcaacg gctgggtttc cacaatgcca ggcaggttgt
    36301 tgaccagttg cgggtctgat gcgaatacgg tgacaatggg cggatacaca gaaatatctg
    36361 tcagacgata tccacttgcg gcacgcccgg agacgatcac ttttactgcc acgtcacgat
    36421 acccaccctg ctgactgact ggcaggctga tgtgaatggc ttcaggtgat acggtaacac
    36481 cttctacagt ttgacctttt tcgtcgagga cttcgacggg aagggtctgg tcaaaatctt
    36541 cgcgcaggcc gctgacattg acctgcacac gtgcccgtgc cactttttcc acttgtgact
    36601 gtgcgcctgc aaccacaact tcggtcgttt ccagaactgg ctcaccgatc tggtatccga
    36661 tcgcggcttc gccggaaagg ctcagattca ggtcgaaagt ttgggtatcg agcgattcca
    36721 gcaaaaaggt cacagtacgc ggagaggcgg agacgatctg taccgggcgg gcatcaattt
    36781 gaatctgcac ttcgctggtg tgctcacctg aactcaaacc cgaaaggtcg agcacggcac
    36841 ggatgctttg cggatcgctt tcgatcacat cccacaccga gcgtggggcg cgcagggtca
    36901 attccatttc tgttggtaac tcactgccca aaacgagatt tgggttttgc cctaccacct
    36961 gaatcggcac cgcagaagga agcgtgcggg tttcatcggg gtcggctgag gtgacggcag
    37021 agatccagac cgccattgcc agcgcaaatg accaaagaaa tgtgcggtaa ttttcagaga
    37081 tccatcgaaa catgtgtaac tcaatttaga cgaacaaccc acagagtttt ctgttcaaac
    37141 ctgtcaagct gtgggggatg ggcgggcaat cctatcacgg tttgtctttt ttggtgggaa
    37201 tcaatccggg gaatagccgt tcaagtagat ttggtttatg gtctttgcca ttggggcgaa
    37261 agaaagcagt gaggatattt tcgagacggt cggggtcaag ccgccgcaac attctgcctg
    37321 catgggcaat ggagatggcc ccagtctctt cggagactac gaccgcaatg gcatcgctgg
    37381 tttcggaggt gccaagcgcg gcgcggtgac gcaatcccat ttgacgttcg ggcgtgcggt
    37441 tcaaaattcc gctggcagag aggggaagta cgcaagcggc tgccacgatc tgtgaaccaa
    37501 caatgataac gccgccatca tgcagggggg tgtttgggta gaagatctga agcagtagtt
    37561 ccggggtgac acgggcatac atttgcacgc cgctttgcgc cagttcatca agggtatcca
    37621 gcctttgcat gatgatcaat gcgccgtgtt gacgggccga taatctggcg gcggcactga
    37681 ccaccgcacg gatggtctgt tcgagggcca catctgttga tttgacagag gcaggccaaa
    37741 tggtcccggc gcgaccgagg cgttccaatg tccgtcgaat ctcgggggcg aagatcactg
    37801 gcagggctag aagcagggca ggcagcgaat tctgcgcaaa ccatgaaaat gcaggcaggt
    37861 ctaccaggct ggtgagcaaa atgagggcca ccacaagcag gatcatgccg cgcagcaatg
    37921 ccatggcttg tgtatcgcgc agtgaataca gcaagccaaa aaatatcagg gtgacgagca
    37981 tgatgtctaa tacgctcagc cagctgattc tttgaaaaat gaagaagacg ttgttgagga
    38041 tttcagtcac ggtaatgggt gatgaataca ggtgtcaggg aaggagatat cctatgatac
    38101 ctgatacctg ccgcccagcc ctgtgatggt tgcataaatt ccgaacttca ggcgagagat
    38161 agccggggaa tcgggattcc gatggtggaa ttatgcgatc gttttaaccc taaatggggc
    38221 gcaagacacg gtcttgacgc agttgtttga acagtaatgc accgccttct gtaagggctt
    38281 cactggctga atcctgccag gcttgaacgc ccagtgtttc gggggtgcgt ttttcatacc
    38341 caagttgttt ccagagttct tcctgggcgg cgagttcgtt gagggggaag atgagggagg
    38401 cctcgctttg taattcgctt gaggcgcgct ccacttcctt gatcagtatt tcgagtgctt
    38461 tttgcgggtt gacgtgctct tcaaggaaaa tatcggtggt gcgggtgacc aggttttcaa
    38521 cctgccaacc tgcaatgcca accatttttc catcgagcaa taaaagcatg taggcttttt
    38581 cgccgaagtc ttccattaca tccaccacgg tgacggtgcg tttgcctttg ctcaagcggg
    38641 tgatcaactc ggcgatggca ggtgagtgtt tgggcttgcc gcgttgaacc gtgaacgtac
    38701 cgttggaggt gacgggaacc ttcgagagcg tttgctcggt atccgcgggt tttgccacgc
    38761 tggctggtac gatttccttc catgctgtgc tgacttgttt ccaaaggtca tcgaaggagc
    38821 cggtattggt aatgacaata ttcgccacgg ctactttggc gctttgagcc gattgcatgt
    38881 gaatgcgttc cctggcctgg tcgcgattca tgccgcgttt gcgaatcaat ctttcgatct
    38941 ggatatcctc tgtggcattg gtcacccaaa tggaatcaca taccttacgc agatcgcctt
    39001 cgagcaactt gatcgcttca atgacaacga cattttgtgt ggagcgctta atgagcatct
    39061 ctgtggcctg acggacgagc ggatgaacga tcccttccag ttgggccaac gcctcggcat
    39121 cattgaagac caggttgcca agtttgctgc ggtctatctc gccgtctttg ttgagcagcc
    39181 acttaccgaa cctgtcaatg acttgctggt agcctggagc gcctttggca tacgcacggt
    39241 gcgagagcgc atctgcatcc acagtgtagg cgccgagatg ctcaagcatt tttcgcacaa
    39301 cacttttgcc ggttgcgatg ttgccagtta gaccaatgac gaatttacca ggccagttgc
    39361 tcacagcttt tccttaatta aagaattttg taatccaacc ctattttaat atcttttcag
    39421 gaaatgtcca cctttatgaa aattgccaac atatgacaag tttttcccag gttgactctc
    39481 aatcttcgag cgtgtatcat cgaatgacct caggttgatc gggcaatcgc ggtcctgcgt
    39541 tgacgggatc gaggaaagtc cgcactccat agagcacgac gtcggatagc acccgggacg
    39601 gggaaccgcc gagaggcgtg aacctgccgg aaaagggcca cagaaacaaa cagccgcccc
    39661 gcccgtaagg gcgggcggtg atggtgaaaa ggtggtgtaa gagaccaccg gcgtttgcag
    39721 taatgcaggc ggccaggtaa cccccgtcgg gagcaaggcc aagcaggggc gaaatcgtct
    39781 gtggacgatg ggagactgct cgtctccgca tgtactcccc ccaaccgtgg agggagtcag
    39841 ccccgggtag gctgcaccga gcagagcggc gacgctctgc ccagagagat gattgcacag
    39901 atggtgcaaa ccattggaca gaatgcggct tatagatcgg cctgaggtgt tttttcaatc
    39961 tgggcgggtt gatccccgcc attttttatt attcaaggtg gcctttgtta tgcaaaaaga
    40021 atcacggctc tctaccattg ttgagcaact tattcataaa tcaatgcaga tcgtatcggt
    40081 acaggttgcc gttggagaat tggttccaat gagcaacgag cagatccatg cccaatggca
    40141 ggaaggacca cttgcgcata ttgttttgaa tatccgtcgt gtgcctgccg agcagcaatg
    40201 catggtttgt tttcaaaaat atcatccaaa ccaaaaggaa gtggcttgtc catactgcgg
    40261 cgcaaatgga gctaaagtga tttccgggga ggaatttttt ctggaagcga tccaggaaaa
    40321 gtaataaccc gttcgaatgg atgaaaattt ctatttccac tggagacttt gtgcatatta
    40381 aaggtaaggt tgtaattgtt acgggtgcct catcaggaat aggtgaagca accgcacgcg
    40441 ctttcggact cgaaggtgca aaagtcgtac tggctgcccg tcgcgttgaa aaactgcaag
    40501 ccatcgctca ggaaattaat gccttgaata cgggtgcaga gactttggtt gtgccaaccg
    40561 acctctccaa actggccgac attcaaaacc tgatcgctca aacccttgca cgttttgggc
    40621 gcattgatgt gctggtcaat aatgctggca ttgggcgagt ggactggctt gaaaaccttg
    40681 accctgtttg cgatattcaa actcaatttg atgtgaatgt catgggtatg atccaggcaa
    40741 cacggctggc cttgccaacc atgatccaac aacgttcagg caatatcatc aacatgtgtt
    40801 ccattgccgg gttggtggct acaccgacat attccattta tgcggcgtcg aaacatgctg
    40861 tgcaggggtt ttcagaggct ctgcggcgcg aggtcaagcc gtgggggatc cacgtctcac
    40921 ttatttatcc tggtggagtt gccacagaat ttgcccaacg tgctggcatc aagcgcaaga
    40981 ccaatgccaa aacaccggct ttcatgctgc tgaccgccca acaggttggt gatgccattg
    41041 tggatctcgt tcgtcgcccc cgccgtatgc gcatcattcc ctggttgtgg gctgtgacgg
    41101 tcttcctgaa taaatttctg ccaggcattg tagatcacac caccattaat cgcttcacca
    41161 ttcccgagcg aaaagacgaa ttggcaactg gatgacatga gaacaatttt gtggaataat
    41221 ataacaaatt tgtgacacat cgtctggagg tagcatgggt ttcttatcca atttgttcgg
    41281 accatcggtg ccaaaacttg gcgcaatgga agtaagtgaa aaaatgaaat ttggaaaaca
    41341 tccgattttg gtggatgttc gccaacctga agaatttcgt caggggcata ttgcaggggc
    41401 gaaattaatt ccgctcaatg aattacacaa gcggatcaag gaattgccgc aaggccgtga
    41461 aattatttgc gtctgcgctt ctggaagccg cagcagttcc gcagcaaaaa tgctgtccag
    41521 ggagggctac accgtatttg atttgcaagg cggcatgctt gcctggcggc acgcaaaatt
    41581 gcccattcaa aaaggctgat aacttaaata aaaactgtcg ggatactccc gacagttttt
    41641 gcttaatccc ttgctcccgt tgctttgatg tctttcaagt tcaattgata cccgatgcgg
    41701 ccattatatt cgttcacttc atagttgaac aaaatatcca cccgtggcgg cattttcttt
    41761 tgccagtcac cgagcttgaa cccgattgca tcgtgggtgt atccttttgc atcttcaagg
    41821 atcaatttca agtgtttcgc atctgacccc actgtgcgcg cgctctttac cttgatgtta
    41881 cgcgccacaa atgatgcgtt tggattccca tatcctgtgg gctcaagata tcgcaatgca
    41941 ttttcaaata actcagggcg gatatcgacg agggaaactt ccgcgtctgc cgtaactgtg
    42001 ggcctgagat cttcgttcga gagtttgtca gcggcgatgg tttttaatcg cgataccaac
    42061 tcgggcaggt tttcattttt taccgtgaac cctgccgctg ctgcatgacc accgtgccgc
    42121 acaagcaggt ctgcacactg gtcgagtgct tcggtgatat ggaactcggg aatggatcgg
    42181 cacgacccgc gtgtttcttc cgcgcctttg taggcaacaa tggatggccg atagtaggtt
    42241 tcagtcaaac gcgaggcagc caggccaacc acacctgagt tgaaatcttc gtgtgcggca
    42301 aaaagcagat aggcatttgg gtcgtcattc attgccatgt cttcagcgcg gctttgaatt
    42361 tcacgcgtaa tacgttgacg ttcgcggttt tgcaagtcta aagtttgggc aagctcccct
    42421 gcgcggaaaa catcttttgt ggtcagcaat tcaaaggcgg ccaacgcttc ttttaatctt
    42481 cctgctgcgt tcaagcgcgg gcctagcgaa aaaccaatgt gtcctgcatt gaccttttcc
    42541 aactttacgg ctgaaacggc ggcgagagag gataatccct gtctcaaggt tcgtttcatt
    42601 tgcctgagcc ctctgcggac caacatgcga ttttcaccga cgagcggggc caagtctgcc
    42661 acggttccaa gcgcaaccag gtccaacaga gagtcgaggt ggggggccag cggtttgcct
    42721 tgaatgctga aaagagcctc agcgattttg tatgcaatgc ccacacccgc cagttcttta
    42781 tctggataga tatcgccgtg ttgtttgggg ttgatgacag ccagtgcagg aggcagttcg
    42841 ccttctgcgg ggtggtggtg gtcgctgata atgagatcca ggccgagggt ccgcgcatga
    42901 agcgcttcat ttggagagcg aatgccgcaa tcaacggtga tgaccagttt gacgttatcc
    42961 gcttgaagtt tgtccatcgc ttcgttgttg aggccatacc cctcttcgaa acgattgggg
    43021 atgtatccgc gcacatcggc attcaggtat tcgagcgcct gcaccaataa agcggtggcg
    43081 gtgacgccat ccacgtcata atcaccgtaa atggcgatgg gttcgtggtg gtcaatggca
    43141 aattgaatgc gttcgatcgc ttccctcatt cccgtcatta gaaacgggtc gttgttggaa
    43201 tcgggtttgg cattcaggta ggcccgcgct tctgcatcgg tggcataccc acggttgaaa
    43261 aggatttgcc tcaaaatgga cggaaatgct tgcagcgcag aatcagcttc gggtgtgatc
    43321 gaggtcggga tgacccagcg tttggtgttc gatgtgtcag gtgtcatatt aaagttgtga
    43381 tgcctggagt ataaacgttc aaccagcggc atggaagggt gctgtataat ttgggcttat
    43441 tcaaaggaga tgataatgaa ggcacttgcg tatgatgaaa agacagcgat ggtgatggtt
    43501 tataccgatt ccatgcttgt gcgtggggaa gtggttgcaa aggaaaatgt gagggtaagt
    43561 atctggctgc gtacgcaggg tgtgcccaat tacatccacc ttcatggggc gcaggtcatc
    43621 tctttcagtg gcggcagcgc aaaaaattat acacaggctg agatctttat tcccacagtg
    43681 caggtttgtg catttcatct ggcgccgcct gctcatgacc cgctagatta tgatgcttcc
    43741 gagatcaacc gtgtcatgca gcctgtgaat attatggtcg gctcttttat gctgcgtgga
    43801 aaaatgcgca tctcttcaca aactgaattt gggaccagtc tcgatgtgat gcgtgcttcg
    43861 tgggcctccg tgtatgaagt agaggtgagc aatccgttcc tgccgcagtt caacatgcag
    43921 gtacccatgc ttctggttaa cccaagccat gtaacttttg ggcttgcata aatgaccttc
    43981 taaattggaa cttggcaaaa caagccacaa tggtataatt cgcaggcttt cggggcgtgg
    44041 ctcagcccgg tagagtgcac ggttcgggtc cgtgaggtcg agagttcaaa tctctccgcc
    44101 ccgacaagta gtaaggagac tcccaacccg agggagtctt ttttgtcgct gttggttttt
    44161 ctggtaaaat gtgcccctgt gttttcagct cgcaagcgca ctctgcctgt ctgaaacaca
    44221 atgtaccctg atgggtagaa aacattctaa aggagtaagt aaaaatggtt cgtcttcgtt
    44281 tacgtcgtat tggtctcaag ggccaaccca cgtaccgcat cgtcgctgct gataaggaag
    44341 ccccacgcga tggtcgcttt ttggaaattt tgggcgtgta taacccacgc accaaccctg
    44401 ccaccatcaa cctcaaggaa gatcgcatct tccactggat gaagaatggc gccctgccca
    44461 ccgaatcggt tgcccaggtt ttcaaggcct ccggtaccca ggcacgcttt gaacgtttga
    44521 agaagggtga gtcgcttgag gcgctcgttg ccgaagctgc tgaggcccag gccaagcgcg
    44581 cggctcccgt caagacccgc aaagattaat cgctttccaa aagagcatta accacagggc
    44641 gtatggatgc cacagagaag atgttgtaca attcttttgt ggtctttgcg ctctgtggtt
    44701 atttttttat caaggcaggt agaaaatgaa ggaactgatc gaatatattg ccaaatccct
    44761 tgttgaccat cccgaggatg tgcaggtacg tgaaagcggc ggtggttcgc gtgtgcgtat
    44821 cgaactcagc gtttcaaagg acgatatggg acgcgtcatc ggcaagagcg gcaaggtggc
    44881 aaactccatc cgcactctgt tgaaggttgc agctgagcgt tcgggcaagc aggcgacatt
    44941 ggatgtgatg gaaccccaat gacggcgaag cagaaatcta caaagcagac agaatcaggc
    45001 tcgcccgcgg gcgagcctgt ctatttggtg atcggctatt tgcgccgctc ccatggtatc
    45061 catggcgaga tcattatgga tctgcacacc gattttccag agcgcatcaa ggcgggacgc
    45121 aagattttta tcggtgaaaa tcaccagccg cacactttgg gcggtgtccg tgaacatggc
    45181 aatggggtcc tggtcaaatt acgcggcatt gaaacccccg aagaggtggg cagattccgc
    45241 aatcagtggg tgtatgtaaa agctgcagat gttccacctt tgcctgaagg gcagtattac
    45301 aaatatgaat tgatcgggtt ggatgtggtg gatgatcaag gcaacgcgct cggaaaactt
    45361 aatgagatcc tggaaacggg tgcgaacgac gtgtacgtgg tcaaaaatga atctggcaag
    45421 gaaatccttc tgcctgccat tcccccggtc attctggatg tggatatggg tcagcgggtt
    45481 atgaaggttc atctgctcga cggtttggtg gacggttaaa tacgacgaag caaaagtggg
    45541 tttatggacg atagaaaata ttgggtcggc ttcaatttga tcaaaggcat tggcgcagtg
    45601 cggatgcagg gattggttgc gtattttggt gaccttgctt ctgcctgggg cgctgaccct
    45661 gccagtttgg ctgaagcagg cctgggggcg aaagtgatcg agcgggttct tgctgcacgt
    45721 caaaaagtgg accttgatca ggtctggaac aagattcaat tacagggcat caaaatcctc
    45781 acctggcagg atgaacaata cccatctcgt ttgaaggaaa ttgaccagcc gccgcccgtg
    45841 ttatacattc gcggtgaata tctgctggat gatttattcg ctgtggcgat cgtaggtaca
    45901 cggcgtgtga ccccgtatgg tcggcaggtg accgaggaaa tttcctcttt tcttgcggcc
    45961 aacggcatca ccgtggtgag cggattggcg cggggtgtgg atgcagtggc gcatcaaact
    46021 gcgttgaagg cggggggtag aaccatcggc gttttaggca gtggtgtgga taaaatttat
    46081 ccgcctgagc atcaaaaact ggcggagcaa atgatggagc gtggtgcggt catcagtgat
    46141 tacgcgcccg gtacaccgcc tgatgcatcc aattttcccc cgcgaaatcg aatcatttct
    46201 ggattgtcat tggctgtggt ggttattgaa gcaggggaga ccagcggtgc tttgatcact
    46261 gcggaattcg ctgccgagca gggacgtgag atctttgctg tgccgggcag tatccttgcc
    46321 ccacaaagca agggtacgaa caagttgatc cagaatgggg ctttgccttt gttgagtgcc
    46381 aatgacctga tgcaggcttt ggatatcacc cgtatcggtg aacataaaac cgcccgaaag
    46441 atactgcccg ccgatgaaac cgaggcgcgc cttttgggag tccttggtca tgagcccttg
    46501 catgtggatg agattcgcat gcaggctgat ttgcccattg aaaaagtatc tgctacactg
    46561 gctctcatgg aattgaaagg tatggttcgg caggttggtg gtatgaatta cgtatcggtg
    46621 cgtgaagcac agtcggagta caaaacttaa ggctcggtgt tgcgaattgc tgttccagag
    46681 tgattcgcaa tgcgcaaagg aatctactat ggaaaatacc tatgcagtga tcatggctgg
    46741 tggtggtggt acgcgtcttt ggcctgtttc gagaaaagaa cgcccgaaac agttgctgcc
    46801 tttgcttggt caagacacct tgtttcaaag tacggtggca cgtttgaaaa acatgttccc
    46861 gtatgagcgc attctggtgg tgacagtgga ggagcaggcc cgcgaaatgc gcttgcaggc
    46921 tcctgaaatc cccgaggaaa attacatcat tgaacccgcg ccgcgtggaa cggcttcagt
    46981 ggtggggttg gccgcggcgg ttttacacaa gcgtgacccc aaggccaaaa tggcgatcct
    47041 gccgtccgat catttcatcc gcaatcgcga tctcttccat tatttgctca atgcagcatt
    47101 tgatgtggcg gaaaatggat atttggttac gcttggcatt acaccaaccc agccttccac
    47161 ggcctatggc tatatccagc agggcgaatc gttggatggc gaatataaat accctgccta
    47221 ccgtgtaaaa cgttttgtgg aaaaacctga tgaacttact gcgcagaaac tgctccatac
    47281 cggcgatcat tcctggaaca gcggcatgtt catctggcgg gcggatgtga tccttgatga
    47341 gatcgataag caaatgccag agttgggcgg ggcattgaag aagatcgcgg ctgcatgggg
    47401 agccgatcag caaggcgctg tcttggatga gaattggcag acgctcaaaa ctgaaaccgt
    47461 ggactacggc atcatggaac atgcggaacg cgtagcggtc cttcctgcgg gtgggctggg
    47521 atggagtgat gtggggatgt ggtcttcgct gtttgaggtg cttcttcctg atatgaatgg
    47581 caatatctcc actaatagca gcctgcattt ggcacatgaa acgcacaaca ctcttgttta
    47641 tggcgggaat agcgatcgcc tgatcgtaac catcggcgtg gagaacatgg tcattgtgga
    47701 tggcggggat gtggtgatga tctgcaagac cgatcatgca caaaaagtaa aagatgtggt
    47761 cgggcatttg aagaaacata atcaggaaaa atacttgtag ccaataactg caggagatat
    47821 ctcctgcagt tcgattttta aggggatttt cgatgtttaa ggcgcaggca aatcgcgcct
    47881 tgaaaaattg catttgatat atttcggaaa attgcgtaga atgcgtgtcc agtatggatg
    47941 gaaatcaggt tttgatatac aaacgcattt tttgcgtgga ggatagatgg aagcttattg
    48001 tatgaaatgc aagacaaaac gggagatgaa ggacccagtt gcaggattca acgcaaaggg
    48061 ttcgcccgtt acaacaggga cgtgccctga atgcggcaca aaactatatc gtatgggtaa
    48121 aactgatgcc catgcggata tggttgcacc tccgcgccct gagaaggtgg ttgtgcgtga
    48181 tggcaagctg gtgatcgttg aatcgcccgc caaggcaaag actgtcgggc gttttcttgg
    48241 caagggatat accgtgcgtg catcggtggg gcatgtgcgt gacctgctca aatcgcagtt
    48301 gtctgtggat gtggaaaata atttcgagcc gaaataccgt gtgcccaatg aaaagaagga
    48361 tgtggtcaag gagatcaaga agctcgcctc gaccgctgaa gaaatctatc tggcgaccga
    48421 ccctgatcgc gaaggcgaat ccatttcatg gcatttgatg gaggccgccc aaatcgaacc
    48481 cgaacgtacc aaacgcgtgg tctttcatga aattactgcc cctgcagtgg cagaggcttt
    48541 ttcacatccg cgtgagatca atatggatct ggtcaacgcc cagcaggctc gccgtgtgct
    48601 ggaccgtctt gtcggataca gcatcagccc gatactttgg gaaaaagtac gtggacgttt
    48661 gtcggcgggg cgtgtgcagt cggtggcact caggcttatc gtggaccgtg aacgtgagat
    48721 cgacgaattc aagcctgttg agtattggtc cattcatgcc gaattaaagc atggcagtgc
    48781 gaagacttcg ttcctttcga aactggttcg tattgacgat aaggaacctg aactaccaaa
    48841 cgaagagacc gtcagaccga tcctggtgga tatggaaacc gccgcctatg ccgtgacaaa
    48901 ggtcaaacgc ggcgaacgca ggcgcaaacc tgcagggcca tttaccacat ccacgctgca
    48961 acaggaggct tcgcgcaaat tgggctttac ggccaaacgc acgatgggtc tggcgcaagg
    49021 cctgtacgaa ggtcaggatg tgggtgaggg cggcacgacc ggtctcatca cctacatgcg
    49081 taccgactcg atgaatgtct cggcgctggc gcaaaatgag gcgcgtgaat atgtgtcggg
    49141 taaatatgga agcgattatc ttcccgctga agcgccgatg tacaagacaa aatctgcagg
    49201 ggcgcaggag gcgcatgaag ccatccgtcc cacctcagtg atgcgtgacc ctgaaaaggt
    49261 aaaggaattt ctcgatcctg ccatgttcaa actgtatcgg ttgatctggc agcgttttgt
    49321 ggcttcgcaa atggaatcgg ctgtctttga caccctgcaa gtggaagtga ctggcaagac
    49381 cagcgcgcat gaataccttt tgcgcgcttc aggctcggct gtgaaattcc caggcttttt
    49441 ggttgtgtat gaagaagcaa aaaatgaaga tgtgaagacc gaagaagacg aagagaatgt
    49501 aaaaattccc gctggaattg ccgaagggca ggagcaggaa ttggtgcggc tcatccctga
    49561 gcagcacttt acccagcctc cgccgcgttt ttctgaagcc tcgctggtac agacccttga
    49621 agagaatggc attggccgcc cttccaccta tgctccgaca atttcgacca ttcaacagcg
    49681 tggttatgtg gagcgtgtgg ataagcgcct gatccccacc gatacaggtt tacaggtaaa
    49741 tgacctgatg gtgcaatact tccctgaagt ggtggatttt aatttcaccg ctcacatgga
    49801 agaagacctc gataagatcg cagacggcga gatgatctgg accgatgcga tccgtgagtt
    49861 ttacaacccg tttgctgaag atgtgaaaaa agcgcaggcc gaaatgcctg tcaccaaatc
    49921 tggccctgaa ccgatcggtc gtgcctgccc cacctgcggc aaggaattgg tcatccgcta
    49981 tgggcgtttc ggtaaattca tttcctgcag tggtttccca gaatgccgct acaccgaacc
    50041 gtggctggag aagatcggtg tttcatgccc gaaagaccac ggcgatctgg tggagaggaa
    50101 gacacgcaaa ggccgcacct tctttgggtg tatgaactac ccgaactgcg atttcacttc
    50161 atggaagagg cccattgccg ttccatgccc caagtgcagc ggattgctgg tgatcgcgaa
    50221 caagcgcgaa gcacaatgta ccaattgttc cgagtccttc ctgctggaag agatcatgcc
    50281 tgagaccgtg gaataaacca tgacaaccgc tctgtaaaat gagcggtccc aacttgaagg
    50341 agaagaaaaa atgcacttta caccagttcc atatctcgac cccggctccg ggagtttcat
    50401 tattcaaatt ttgatcgcag ccttgttagg cctgggagtt gccttgcgtg cttcatgggg
    50461 caagatcaag gggtggtttg gaatcaagcc gaaagcggaa gatgaggatg gcgaatctgc
    50521 cgatgccagc aactgaacag ctttccgctt cttttcgtga cccaagcggg tttctgtttt
    50581 ctcgtaacgg ggtgctttac cgacaaatca accgcgccta tgagaaggat tatgcgcggt
    50641 taatggattc aggtctgtat gagaagctgg tcaaggctgg gctgctgatt ccgcatgttg
    50701 agtcggatca ggctccagca gagtcggatt cggtttttaa agtcatccag cctgaacggg
    50761 ttccgttcat ttcctacccg tatgaatggt cattcagcca attaaaagat gccgccctga
    50821 cgactctggc gatccacaaa cgcgcaatca aggcgggcat ggccttgaag gatgcaagtg
    50881 cgtacaacat tcagtttgtg cgcggaaaag caactttgat cgatactctc tcttttgaga
    50941 tttataaaga gggtgagccg tgggtggcgt atcgtcaatt ctgtcagcat tttcttgcgc
    51001 cgctggcttt gatggcttat tgcgatgtgc gtttgagtca gctgttgcgg gtgtatattg
    51061 acggtgtgcc gcttgatctt gccagcaagt tactgcctgt aaaaacgaag ttcaattttg
    51121 gcctgcttac acatatccat gtccatgcag gtgcgcagaa gcgatattct gacaaggccg
    51181 ttgagactcg caagggaacc acaggagtga gtcagcaggc aatgatcggt ttgatcgaaa
    51241 gcctcgaagc cgctgtcaaa aaaatgacct ggaaacccgc tggcacggaa tggggcgatt
    51301 attacgaaaa tacaaattat acagatggcg cgttcgagca taagcggcag cttgtgcgtg
    51361 aatggacgct tgagaaaaag cccacactgg tttgggatct gggcggcaat acgggtgtct
    51421 ttagtcgtga ggcggtttct tccggtgcat tcgcggtttc ttttgatatc gaccctgccg
    51481 cagtggaaca aaactatcga accgtaagat caaaaaagga acagaacatc ctgccgctgg
    51541 ttttggactt gaccaacccc agtccgtctt tgggctggga tcacatggaa cgcgactcgt
    51601 tcggtgcgcg cggccctgtg gacatggcct tggcgctggc ggtcatccat cacctggcga
    51661 tttcaaataa tgtaccgttg ccgcagttgg catcgttttt tgcggcgcgt tcgaaatggc
    51721 tggtcataga attcgtccct aaatctgatt cgcaggttcg caggctgttg acttcgcgtg
    51781 aagatatttt tcccaattac acccgtgaag ggtttgaaag cgctttttca aaccgcttca
    51841 ccattcataa agcggaagcg gtgcgagatt cagagcgtat tctttactta atggaagcgc
    51901 ggtaaccatt ctgatatgtg ggtggtaaaa aattggatgt ataattgccc aaataacatt
    51961 atacaaagga gcctcacatg cagtttgttg tagcattctt gaaaaaactg ccggccgtcc
    52021 caattgcgct gttcgttggc gggttggttt tagggctggt gtttgggtgg gtgatctggc
    52081 ccacggagta cacaaataca accccagatt ccctgcgcgc cgacttgcag gaagactatc
    52141 tgcgcatggc gatcgactcc taccgtgtaa atcttgatcc tgaccttgcc attcaacgtt
    52201 ggttcaacct ggggataggt gctgatcagg catttaaaaa tattcaggcc aacccgggca
    52261 agctggatcc cgcagtaatt caagcatatg gcgaaacagt tacgaagatc cttgctacag
    52321 gtgtgcagcc accggtaaat acaggcggca gcgctacgct gcttaggagc atcttcctgt
    52381 ggggtggtct gctcgttctg ttagggattg gcggttatgg cggctggtat atgtatcgtt
    52441 tgcttggcaa gcgtggcagc ggcgaagtaa cacccaccat gcaggccgcg gaactcaacc
    52501 gcagtgcaga gaagaccaac ttcgaacagc ttgggctggc tccgcccatt acacagacca
    52561 tgaccacata cgtgctcggt gatgatcttt atgatgaatc cttcagcatt gatacacagg
    52621 ctggcgaatt catgggcgaa tatggtgtgg gtgtttcaga ggccattggt gtaggtgagc
    52681 cgaagaaggt cactgcactg gagatttggc ttttcgacaa gaacgatatc aagactgcca
    52741 ccaaggtttt gatgtcacaa catgctttca acgacccaac gatccgcgcc cgcctcgaac
    52801 ccaagggtga gttgatcctg gttgagccgc aagctcaggt gctgttggag accgccaccc
    52861 tgcagttgct cgcaaccgtt gtggacctgg agtatggtca cggcccccaa ccagctgaca
    52921 gttatttcga acgcattacc ctcgaattgg ccatctggcc tcgacaggga taatcaaaac
    52981 ataaaaaaat ccgcctccat tttcggaggc ggattttttg ttattcaatg ctgataatct
    53041 tgaatttgat cttcccaccg ggcgtttccg cttcgacaac ctgaccgacc tttttttcca
    53101 tcagtgcttt gccgatgggg gactcgatcg aaatctttcc cttgcgcgga tctgcttctg
    53161 tagcgccgac cagatgatag gtctcagggt cataatcggc ttcttgaatg gtcacgcgtg
    53221 agccgaccga aaccacatcc ttggtttgag aattgtcaat aatgacggcg ctcctcaaaa
    53281 tggcttcaag ctcctgaatt cggccttcga gaaaaccctg atcttccttg gctttgtggt
    53341 aatcagcgtt ctctgaaagg tctcccattt gaatggcaga acgtaatctt tgcgaaagtt
    53401 cctcgcgccg ggggcctttg agttcctgca attcagcggt gagttttgct tcgccttctg
    53461 gagtaaggta attaatggat gacatggtaa tcgctccttt ctgaaattgt tataagggtg
    53521 gtttgacggg gacgaaaata actttgttcc cccttgattg aaaagattaa ggcttttcag
    53581 aggggttgaa cagcttttga tgttcttcaa cggcatagcg gtcggtcatg cctgcaatgt
    53641 aatcgcagat ggtgcgttcg agtccgcgct tttcgatgaa gaattggaca tggtcaggca
    53701 gcatggacgg ttccgcctga taggcatgga acaactcaga gatgacccgc tccgctttga
    53761 tctgcatccg caccacgcgg taatggcgat agagcttttt ataaagcagg tctttcaatt
    53821 ctcggttacg gcgctgcatc tcttcgctat aaccgatgat gttgtacttg agcttttgaa
    53881 tatccaatgc ggtctttgcc ttgctgtctt ttaagcggat atctgtcgct tgcagcatat
    53941 cggtgaccat cagcccaacc agttgacgga tcatgcgatg gcgttccatt tcgctcaacg
    54001 tatgtccctg ccatttgtag gtttcgcgca ggatttccca cagcgagatg ccttcaagga
    54061 tgtgtggggt gatcattccc gagcgcaggc cgtcgtcaag atcatgtgtc gtgtaggcaa
    54121 gctcgtcggc gacatttgca atttgagttt cgagatttcc gcgcagatcg gggttgaaat
    54181 cccgtgcgtc agagatatcg tattccgatt cgtgcttgac cattccctcg cgcacttccc
    54241 aggtaaggtt taagccaggg aattcgggat accgctgttc cagttcggtc acgatccgca
    54301 gagactgttt gttgtggtcg aagccgccaa attccttcat cagccgtgcc agtgcaactt
    54361 cacctgaatg tccaaatggc gaatgaccaa gatcgtgcgc caggcagatc gcctctacaa
    54421 gatcctcgtt ggcgcccaag gcgcgggcca atgtccgccc gatttgagca acttccagtg
    54481 tatgcgtgag acgggtacgg aaatagtcgc cttcgaaatt gataaaaacc tgcgtcttgt
    54541 attccagcct gcgaaaggca gtggtatgca gaatacggtc acggtcacgc tgaaaggaag
    54601 ttcgatagtc gggttcgcta tccagatagg cgcgtccctt cgaatccttg ctgcgcattc
    54661 cataggaagc aaggctttta tcttcgatct cttccaattg ctgacgggtg aacatgattt
    54721 ccttttccga ttctacagcg tgccatggcc ttagacaaaa ctgtcattgc gtgctttgag
    54781 ttttctcaag ttctacgcaa tgacattgaa tttgtggggc gagagggggt cgaaccctca
    54841 cgttgttacc aacgacagat tttaagtctg ttgcgtctgc cgattccgcc atcgccccgg
    54901 cgattcatat tttaccttaa tttcacgctc atgcattcaa tggctctcat gacaagtgaa
    54961 taaagcacat taattatcta catgtattgt ctccattctt tgccagctcc gttttttaag
    55021 ttgcactttg gtctaaaatc gagtaaattt acctgatagc acgtacgttc tacacaggag
    55081 aagattcatg gcagactcaa atttttacct tggccgattg tttgatacaa aaacagcgaa
    55141 ggtcaccact caacccgttg aatatgaccc cgcagatctg accacccacg ctgttgtgac
    55201 aggcatgacg ggctctggta aaacaggctt gtgtgttgct ttgctcgaag aagctgcttt
    55261 gcaaaatgtc cctgccatca tcattgaccc taaaggcgat ctaaccaacc tgcttttgca
    55321 tttccccgac ctgctcccgc aggattttca accgtggatt gaccccgaaa tcgtccgccg
    55381 cgcaggcacc acccctgaaa gagctgcaca ggaagccgcc gaatcctggc gtgatggact
    55441 gaccgagtgg ggcattggtc aagaccgttt gctggctctg aaaaatgccg cacagttcgc
    55501 catttacacc cctggttccg attcaggcat ccctgtcagc gtactttctt cgctcgctgc
    55561 acccgatctc gattgggaga ccaaccgtga ggtcctgcgc gaaaagattt ccagcaccgt
    55621 caccgccttg ttgggccttg tcggcatgac cgatcttgac ccgctccgct cgcgcgaaca
    55681 catcctgctc tcgaacattt tcgagacgca ctggatcaac ggcaaagacc tcgacctcac
    55741 cgaattaatt ctacaaacac agaccccgcc attccaaaac ctcggtgcct tccctgtgga
    55801 tacattcttc ccgcccaaag accgcatgga actggcaatg gttttgaaca acattctggc
    55861 tgcacctgcc ttcgaaacct ggcgtgaagg tgaagagctt gatgttcaaa aactgctatt
    55921 catgcccgat ggcaaaccgc gccacagcat cttctacctt gcccacctct ctgatggcga
    55981 gcgcatgttc tttgtcacac tcctgctttc cgctgtggaa acatggatgc gtacccaaaa
    56041 aggctccacc gctctgcgtg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56161 nntctacggc tatcttccgc ctactgctgt gccgccctca aagggtccat tgctgcgcat
    56221 gttgaaacag gcccgcgctt ttggcctcgg ccttttgctc gccacccaaa accccgtgga
    56281 tatggattac aaagccctct caaacacggg cacatggttc attggcaaac tgcaaaccga
    56341 acgtgacaag aaccgtttgc ttgacggcct cgaaagcgcc gctggcggta tcccgcgcgc
    56401 tgcgatggat aaactcattt catcgcttgg caagcgtgta tttgtcatgc acaatgtcca
    56461 tgaaaaaatg cccgtactca tgcaaacccg ttgggcgatg aatttccttg caggccccat
    56521 gacccgagca cagattcgtg atctcaatct tttggctgat gcagattcaa cgcgtcgcgc
    56581 ctcgccgatg cctgctcctg ctccgaaaaa agagtcagca cctcaacctt ctgtagaggc
    56641 cttccagcct gtttcgatcc cctcggcctc acaacccgct actccgcaac ctgcaacgct
    56701 gcaatctgct cctgcgcgcc aatccacacc cgcaagtgga tttacgacca aaccctccat
    56761 cccagctggg attcgtgaat actacctccc acaaaattac agtttgcccg aagccttcca
    56821 ggcggctggg cgtgccatgc cttcacaggc catgattcag ggaatcatat accgtccatc
    56881 tctgttggca tccgcccaag tgcgcctgct tgaccgtaaa cacggcgtgg attccgaact
    56941 cgacaaagtc gtactcgtgg atgagatgga ccgtcgcggc attgtccgct gggaagatta
    57001 ctcctatggc ggcccctcgc ttgacaggat cgaagccatg cccgctgcca ctgcccgctt
    57061 cagcaccatt gatgcgcctc tcaataacac aaaaatgata gccgccctgc aaaaagattt
    57121 taccgattgg gtcttccgca actccgcagt cactgcccgc gccaatcagg cgctcaaagt
    57181 atttgccggc ccagatgtca gccaggctga gttcatgaaa acctgtgccg ataccgcccg
    57241 tgatgcccgt gatgcagaga tcgcaaagaa gaccgcctcc atcgaaaaga agatcaaagc
    57301 ccttgaagac aaactcgccc gcgaagagcg tgaactgcgt caggacgagg aagacctgca
    57361 aaaccgcaat atcgaaacag gcatcaacgg tatcgaagtt gtagcgggtc tttttggtgt
    57421 cggccgcaag aagagcgtct ccactccgat ctcaaaatat cgcatggcac aaaatgccaa
    57481 agccgaggtc caggaatcgg tggatgccat caatcaatac agaaaagatc tgaacgccct
    57541 gttgcgcgaa cgcgatgaca tgaccgctga gatcaacaac cgctggggga gcgtggtcaa
    57601 tgagatcagt gaagtaagca tcaaacccaa gaagaccgat atctatgtca acctctttgg
    57661 cgtggcttgg aagccgtact atctcgttca gtctggcgat gagaccattg aactccctgc
    57721 ttttggtgcg gagtaattta atgcattaag cagatttttc ggcctgacat ggttttgaat
    57781 gtaaatagtg acgacctcag gtcgtcacta tgcattaaag gaaaaaatac aatgagcatt
    57841 ttcattcttc ttctcgcgct tgccatttgg ggcatcgtac attcttttct ggcctcccat
    57901 tttgccaagg gcatgatctc cctaaaaaca ggcggcgcgg atttttaccg cctcacctac
    57961 aatgtctttg ccctgatcag tttcgccccc atcctgtacc tgatgcgtac cctgcctgac
    58021 cagcccatct atcaggtcgc ctctccgtgg aacctggtca tgtttggagg ccagctgttt
    58081 gccctgctga tgctgttgat cgccttcctg caaacagatt cattgtcctt tgttggctta
    58141 cggcaattgt ttgaaaaaga aaccacgggc gccctcgtca cccgtggtat gtatcgaatc
    58201 gtgcggcatc cgctttacac ttttggtttg ttgttcatct ggttgacttc aaccatgacc
    58261 cagaattcat tgaccgttta ttgcggtgcg actctctata ttttgatcgg cacttatttt
    58321 gaagagcgca aacttctgct cgattttggc gaggcctatg cggagtacca gcgcaaaaca
    58381 ccgatgctga tccccgggct tgtgctcggc agaaaataga aaaatcccgt ggatttttgc
    58441 ctcaatggct tctgtaccct tttttgagtc cttcgtttag ggctttgtaa ttttcggcat
    58501 agaactctgt gtaaccgcac gcactgcaaa cattggcgcg aaaatggctt tgtgcgtttt
    58561 tggggagcca tacgaaaggt cgtttttctg gttcaggttc aatgatgctg acgtaaggcg
    58621 gatgtccctc accgccatgc acattcatcc ctgaaaaaac ttcgttggaa ccacactttg
    58681 ggcacggatt catattcacc tcgcaaaaag acaattatct aaaaatggcc atgatgctgg
    58741 caaagacacc gatcaaaacg gggatcaagg tcacaagaat gatgaccgtc accaaagccg
    58801 tgatcccgca tccgatccac atggacatgc gggtaacatt tttgacggtt tcgttggcct
    58861 gtgccatcat tggcgcatac atctgttgaa tgttctgcgc cgtgaccatt tgagtgtgcg
    58921 ccagatgatt cacagtgtat gtggctgaag tttcgtcgtt gcctgtcaat gccatgtagc
    58981 gtttgacggc ttccgcttta ttgccacttt gcgccagttg aacgacctct ccccattgtg
    59041 tgccaaaacc aaccatggcg cttgtatcaa cgcctgcaaa gatgctcggt tgggaatagc
    59101 ccgtgctttg cttttgtacg cgcaggcttt caggaatggc cacaacactg ccgcagtaca
    59161 gacacttcat ggtgctttcg cctgctggcg gttccagcgg cccaccacac gacgggcatt
    59221 ttagtaattg catcacttca gacattggtg taaacctcca aaaatagatc ttgccgaaaa
    59281 acgatgcggc attatcccct gattgtctgt ttttcgaaac cagacaaagg tatgactgtt
    59341 tggtaaactc acttcgatga aacgcttcgc cgcccttacc ctgacgattt tcctcatcgc
    59401 ctgcacccgt tctgtaacgg gatcgtacac ccccatcccc ttgtattcga cgaaaatacc
    59461 gatcactccc accctgaatc ctgttcctgt tgcgacagag acagccaccc cgtcatgtgt
    59521 tgatgcgttg ctgcaggtga agatcatgcc gcttggtgat tccatcacct atggcgaagg
    59581 cgatccgaat cgcggcggat atcgtacaaa actggccgag tttcttttgg aagacggcat
    59641 cgcgttcgat tttgtcggtt cgcagcgcag cggggaggct gtactggcag acccggacaa
    59701 tgaggggcat ccgggctgga ggattacaaa catcaaagat gcgattgcat cagaaggctg
    59761 gctggaaacc taccagccag atatcattct gctccatatt ggctccaatg acctgcgcaa
    59821 tggcagggga atttatgccc gcgaccatct ttcggctttg cttgacgata tccttgtgcg
    59881 cctgccgcat gtacatgtga tcgtggccca gatcatcccc acgcgctggg gttctgagat
    59941 cgaccaccaa atttataaca atgcgattcc agatgtggcc gcttcaaaag gcgggcaggt
    60001 ctcggttgtg gatatgcagg agatcctggt caaggatgat ttcatgacgc tttatcaccc
    60061 cagcacaagt ggatacgaaa aaatggcaca ggcctggagg gccgccatcc atgctttggg
    60121 gttacaaccg aactgtgcaa tttcacaaaa ataaattcca tcggggtggt tgggtaattc
    60181 tatttaaact acaaaggcac tgagatacag agtcgatcat tattctctct gtgtctcagt
    60241 gcctcgttag tttggctttc tggccttttc tttatgggat tgttgtagcc agcttgggca
    60301 gtgattgtag atatgcccac aacgctttca attcatcgtc ggagaaaaac ttatagtgct
    60361 cccacggcat ttgcgcaggg tcaagtttgt gtccgctctt ggcctcacct tcgcgaatgg
    60421 ccttgataaa gtctgcttct tcccatgcaa ccaattcacc accaggggtt aggttggggg
    60481 cattgatcgc agaagggtca gcgcttttgc cgccagaaag atccgcgcca tggcagtcac
    60541 ggcagccgct cacgttgacg atgtattcgc cgtattccgc agtcactcct gcaggcggat
    60601 acgaaacatt ttccaatgga ttgtcaataa tataagcagc aacaaccgtg ctttccccaa
    60661 aggctcctgc tcccatcagt gccttgccca tcaggttaaa gctggggtcg cgggtttctt
    60721 tatcaacggg cggcagggtt ttcaaataag cgacgatatc accaaggtct tcgtcactgt
    60781 aatatctgaa aaactgcgca ggcatgatca gcagactgtc accctcagaa ctgacaccat
    60841 ggcggagtgc gcgcacccaa tcatcatcag tgaagtcact gccgaccccg ccattgccgg
    60901 gggtcagatt ttttgcatca atatacccaa agggtgcggc aaagaattca ccgccagata
    60961 gatcgtgacc atgacagcca atacattctg ccatcaccca atgctcgccg cgggcaacag
    61021 cttctgcatc ggtggggatg gtgattttct cgacctgtac atcgtacttc ttgccaagca
    61081 tggaattgcc tttgagagcc agcccaagga atgcgatcaa tatcacacca agcaggcttc
    61141 caagcacaat accgagccat ttgaaaaact tcttcatatt acattctctc ctcattaatc
    61201 tataaaattt tcattccaac cgataatacg accattttta tctatttaca agccatacat
    61261 acacacaact ccattacatt ttggaatgaa atgacactca cgaaaaatat tggcacaacc
    61321 catttgacat cctcctcctt ttttgctaaa ctagaagttg tcagacaact acgccaattg
    61381 caacttgcca aaggcagcct tttcccatga caactctact cataaaaaat gcctatatcg
    61441 tcaccatgga caatcaccaa cgggagattc ctgaaggtgg catcttcatt cgcaatggtc
    61501 tcattcaaga agttggtccg gtaaatgaac taccagccac agctgatgaa gttcttgatc
    61561 tcaagggaca tgttgttttc ccgggcctcg tcaataccca tcatcatttt tatcaaacac
    61621 tcacacgtgc cgtccccgcc gcacaggatg caaatctatt caactggttg aagacccttt
    61681 acccgatctg ggcgaagctt caaccggatg acatttttat ttcgactcag accgcactgg
    61741 cagaactcgc cctgtcagga tgcaccaccg catcggatca tctctacctg tttccaaacg
    61801 gttcaaaact ggacgatgaa attgccgcag cctccgagat cggggtccgc ctgcaggcat
    61861 cgcgcggttc catgagcctc ggtgaatcca aaggcgggct gcccccggac tcggttgtgg
    61921 atagcgaaga gaatattctc aaagattcac aacgccttat tgagaaatat catgatgcga
    61981 aacccggtgc aatgacccag atcgttcttg caccctgttc ccccttcagt gtcacctctg
    62041 acctgatgaa gcaatctgca aaaatggcgc gcgaatatgg cgtgcatttg cacacccatc
    62101 ttgctgaaac agaagatgaa gaaaagtttt gtatgcaaat gtttggtcac cgtcctgttg
    62161 gatacatgca ggaagtggac tgggtggggg aggatgtctg gtttgcgcat gcagtttggg
    62221 tgaatgatga ggaaattaaa gtctttgcca aacataattg cggtgtggca cattgcccca
    62281 cttcaaatat gcgccttgcc tcaggtattg cacccattaa ggaatatcgc aaggcaggtg
    62341 taaacgttgg cctcggtgtg gacggctctg cctccaatga tggttcgcat ctgctggcgg
    62401 aagtccgcaa tgccatgctt gtctcccgtg tcaaagaagg attgaccgga tactcccttt
    62461 ccaacgatcc caaccgaaaa ttaatgaccg cccgtgaagc tcttcacctc ggcacacgtg
    62521 gtggcgcagc tgttttgggg cgaaccgaca ttggcagcct cgaagctggc aagtgtgccg
    62581 acttctttgc tgttaacttg aacaggctcg gctttgcagg catgcacgac cccgtctctg
    62641 ctgtggtttt tgggcagccc gttaacgtgg attacacagt tgtgaatggg aagtttgtcg
    62701 taaaggaagg tagggtggta acgatcgacc aggagaaatt gatcgagaaa cataacaagg
    62761 ccgccaaacg acttttggct ggttgagagg agtctgaaac accatgttcg atagtgtatt
    62821 gccaattatt ttcagctggc cttttgttgt cttttctatc atcgcctcag tggtggggat
    62881 cctttatgaa atgccgctgt tggttgttgt gggggcgttg acgattgtgc cgttttctta
    62941 tcaattgaat aacgtgcaat attttcgcgg ttttgccttg cttttgccgg tgttgcagct
    63001 cttttctgca tgggcgctaa aagaggaaag aaatgtttgg gcatgggtgt tgtttgcacc
    63061 caccattgcc atcattctct ggttgttcgt ggtcaattcc atagtcggat ttttctagtt
    63121 tgttctggga gattttttat gacaccgtcc acaaaactca cccgctccct cgttgatgtt
    63181 gccatgggac gtacccctgc cgaccttgtg atccgtggtg gaaagtgggt cagcgtgcag
    63241 tcgggtgaga tcattcccca tactgatatt gccattgtaa atggacatgt agcctttgtt
    63301 gggcatgatg ccagccatgc cattggtaaa aagacaaagg tgatcgaagc gaacgggcga
    63361 taccttgtac ctggcctgct cgacggacac atgcacgttg aatcaggcat ggtcacagtg
    63421 accgagtttg tacggactgt ggcagtacgc ggtacgaccg gtatgtttat tgacccgcat
    63481 gagatcgcaa atgtattcgg cctcaaaggt gtgaagttga tggtggatga ggcgcaaaag
    63541 cagcccatcc atgtttgggt gcaaatgccg tcctgtgtgc cttctgcgcc agggctcgag
    63601 actccggggt catccattgg gccgaaggaa gtggccgaag ccatgaaatg gaaaggcatc
    63661 atcggtcttg gtgagatgat gaatttcccc ggtgtattca tgggcgataa aaagatgctt
    63721 gacgaaatgt ccgccactca tgcggcaggg aagaccattg gcgggcatta tgcttcgcct
    63781 gatcttgggc tgccatttca tggatatgtg gctggcggcg ccgaagatga tcacgaaggc
    63841 acacgtctgg aagatgcgat tgcacgggtg cggcagggca tgaaagccat gctgcgttac
    63901 ggttcggcct ggcacgatgt agcgaatcaa gtgaaggcga tcactgaaaa gaaaatggat
    63961 tcgcgccgtt tcattctttg cacagatgat tcgcacgcgg aaacgcttac agtggaaggg
    64021 cacatggacc gtgtgctgcg ccatgccatt gctgaaggct tgaacccgat ggcggcgatc
    64081 cagatgatga ccataaatac cgccgaacat ttcggcttaa cgaaagaaat gggcatgatc
    64141 gccccggggc gctgggctga tgtgcttttg gttgaagacc tgatgaactt taaagcggat
    64201 gttgtaattg ccaaaggaca ggtgatcgct gaaaagggtg aattgctggt caaacttcct
    64261 gcggtgaagt acccgcgttg ggcaacgaga tcggtgcatt tgaagcgacc tctcaaggcg
    64321 gaggattttg tccttcggac gagagcagct cacgggtccg atgtggatgc gcatgtcatt
    64381 ggcgtgatcg aaaatcaggc gccgactcgt catctgcgga tgaaggtcaa agctgaaaat
    64441 ggggaagtca aagccgatat gaaacgtgac ctggcgaaga tcgctgtggt ggaacgtcat
    64501 cgcgcgactg gcaaggtgat ggtggggttg atcagcgggc ttgggtttac gcgcccctgc
    64561 gcaattgctt cgactgtggc gcatgacagt caccatatga tcgtggtggg tacggatgat
    64621 gggaacatgg cgattgccgc caatgaactg gctcaatgtg gaggcggtca ggtggtggtg
    64681 atgaatggtg aagtgatcgg gcgcgtggaa ttgcagatcg ctggcttgat gtccagtgag
    64741 cgtgcggatg tggtggcgaa gaaggcggga actgtgcttg atggattcaa aacttgcggt
    64801 agtgagttga ataacccgaa tatgcagttg agtttgtctg cccttgtggt gattcctgaa
    64861 ttgcggattt cagacaaagg gcttgtggat gtgacgaatt ttgagtttat ctctgttttg
    64921 gatgagtgag gcagatatgt caaattttcc ttttcaacga ttgcaggtgg atctggctgc
    64981 attgatcgcg cgaacccctg cggggcagcg tctgccatca gagcctgaac tggcgaaaca
    65041 aatgggtgtt tcccgtgcca cgctccgtga ggccatgcgt gcatttgaaa cccagggttt
    65101 gatccgtcgt cgccagggtt cgggcacgtt tgtggtgggg aagatgcggg gcttgcttga
    65161 tagcgggctg gaagttcttg aaagcctcga aaccatggca aagcgtttgg gtttggaagt
    65221 ttcagtgagc gacctcagtg tagattcgct tctggcagat gccgatgcag ccactgccct
    65281 gggtgtcgcc gtggattccc ccctgacacg cgtcaaacgt gtggtgcgtg ccgataacag
    65341 accagttgcc tatcttgtgg atattctccc ggaagcagtc ctgcgtgttg aagatctgcc
    65401 tgctgatttt cacggttctg ttctggactt tttgcttgat cgcggcgatc ctttgaagac
    65461 ttcccgcgcg aatgtcagtg ccattggtgg aacggcagat gttgccaagg cgcttgaaat
    65521 ccagcgcggt gacgtgttgc tccattttta ttcgcaattg tttgatgatg aaggcaggat
    65581 cgtggattat tcgttgagtt atttcatccc cggctatttc catttccata ttatgaggcg
    65641 tgtcggcgga taaggtgctg tattttacaa cgctggggtg ggcccgacgt tgaatcattt
    65701 ttatgcaaca aggagttcaa atgtcaattc aggaaataca aaaaagagtt caggcttccc
    65761 gtgaggatat catcgagttt atgcgcgaga tcgttgccat tccctcgatg gattcccagt
    65821 tgaaggatgt gggtgaacgc attgccaaag agatgaataa acttggtttt gatgaggtcc
    65881 gctttgacaa gatgggcaac atcatggggc gcattggtaa cggaaaacgg gtcattgttt
    65941 acgattcaca tgttgatact gtcggtattg gcgacccgca cgaatgggaa tgggatcctt
    66001 tcaaaggcaa gatcgaggac ggcattttat acgcccgcgg cgcttgtgac gaaaaaggct
    66061 caacccctgg catgatctat ggtctggcca tggcccgtga tctgggtctg ctcgaaggct
    66121 ggacagccta ttacttcggt aacatggaag agtggtgcga tggcattgct cccaatacct
    66181 ttgttgaagt ggacccaaag gtgaaacccg attttgtggt catcggcgag ccgaccaaga
    66241 tgctggttta tcgcgggcat aagggccgcc ttgaaatgaa agtgaccgcc aagggcaaat
    66301 cggctcatgc ggcgagcaat catctgggcg ataatgccat ctacaaactc ctccctgtca
    66361 ttgcagggat tcgtgacctc gaacccaaac ttggcgacca tgaatttttg gggcatggca
    66421 agatcaccgt ttcagacctg catgtaaaga ccccctccat caatgctgta cctgatgaag
    66481 ccgttatttt catcgaccgc cgtatgacct ttggtgaaac caaggaaatg gtcaaaaagc
    66541 aggtggaaga tttgatccct gcagaattca aagattctgt gaagctcgag gaattgttct
    66601 acgatgagcc ttcctacact gggtttgtct tccctgtgga taaatacttc ccagcctggg
    66661 ctttggaagc ggaacatccg ctggtgcagg ctggtcagga agcccgcaag cagattggcc
    66721 tgcccgaggc agccagtggc aagtggaatt tctccacgaa tggcatttac tgggctggca
    66781 aagctggcat tccgtccatc ggttttggcc ctggcgatga agagaccgca cacactgtcc
    66841 gcgattctgt ttcgctggaa gatatggtca aggccaccga gttttatgcg attttgccga
    66901 gcctgattaa ataatagatt tgcagggaca cagtgatgct gtgtccctgc atggtgaata
    66961 tgaaaaccct cgaaatccgc cgacactcca tccgcgatca tgcgggtgac catttgaatc
    67021 aaaacgggat aacgctggcc cgtttgatcg gtcagaatag cggcccattt gatcgtgtgg
    67081 tcacttctgc gttgccgcgt gcctttgaaa cagccattgc catggggttt gctgtggatg
    67141 agcaaactga actgttgaac acctacggcc atgccgtgga aaaagaagcg ccctggccgc
    67201 agaccttcga cttttacgcc gaagtgatca agcataacgg tgcacctgcg cggtatgccg
    67261 gtaaactggc caagttccat gccagcatta tggattttat ctccgagggc cgttcggcat
    67321 tggtggtaac ccatggaggt gtggttgagt tgagcattgt tggctgcctg ccagggtttg
    67381 atttttcaca atggggcgac ccggtggact attgtgaagg cgcacggctt tattgggatg
    67441 ataagtttgt tgatgcagaa gtattgcgcg tcaaccgata aaaatcaagc gaaggaactt
    67501 tataaacaat gaaaagaaag tcagtttttt tcacatctct cctgctcgtt atggcgctgg
    67561 cagtaactgt attggctggg tgcagcagtg ccaagcccaa ggtcgcgctc atgcttgcgc
    67621 gtggcggact tggtgataaa gcattcaacg atagtgccaa tgaaggcctg caaaaagcca
    67681 tgcaggaact cggcgtggag gtgaaaactt tcgattacca gcaggatacg caaagtgaaa
    67741 acgtccgcaa agccgcacag gatggctatc aattggtcat tgggcttggc tctgaaaact
    67801 ctgcagccat taccgaggtt gcgaaggaat tccctgatac caaattcgca ttgatcgatg
    67861 caacagctga aggctccaat gttacttcgg tcactttcag tgaactcgaa ggtgattttc
    67921 ttgcgggcgc attggcggcc ttgttgagcg aaaataacaa agtggcctac ctcggcggcg
    67981 ccgatgtgct ggtgattcgc cgcattcaat tcggttttga gcagggtgtc aaatacgtca
    68041 agccagatgc cgagatcgtt gttcaatatg ttgctggcaa ggatgatttc agcggtttct
    68101 caaaacctga ggatgccaat gcaattgccg cacaaatgta tgccgatggc gtcgatctca
    68161 tttacgcagc ggcaggagga tccactctcg gggcgatcga tgccgctaaa gcagcgggca
    68221 ggctcatcgt cacaacagga agcgaccagc gttatctcgc tcctgaggtt gtggtcacca
    68281 gccgcaccaa gaacatgaat caggccgtat tcatgttgat cgaatccttc acaaaaaaga
    68341 ccttgcaaag tgggacgatc caactcgact acaaatctgg tggtattggc atcgcgcctc
    68401 tttctgctga ccttgtacct gcttcagttt taagcgcatt cgaagccatt caaactgatc
    68461 ttgagtctgg aaacattaag ttggaacctt ttgttagtca atagttggct ggtcgctggt
    68521 cctctggtca aagagaagac atggcaacaa taaaaaattt tgaagatatt gaatcttgga
    68581 aaaaggcccg cgaattggca ggctacgttt ataccctgac ccgaaaagga ggttttgaac
    68641 gtgacttttc cttgaaaaac caaatacagg atgctgctgg ttctgttatg cataatattg
    68701 ccgaaggctt tgaatcaggg tatgacccag agtttgtccg ttttttgaag atggcacgtc
    68761 gttcagctgg agaagttcaa tcagaactgt atcttgctct tgatacaggt tacatttcac
    68821 agaatgaatt ggagaaggcc tataagcttg ctgttgatgc caaaaaactc attaatggtc
    68881 tgatctctta tttgagcaaa gaccaaaaaa caaaagacaa gtagacaaaa aaaacaaaag
    68941 actaggagac taaatcatgc aaaccaattt tagaggccgc gacttcattg gcgacctgga
    69001 tttcacgaaa gaggaagttg agaccgtttt ggaggtggcc tgggatctga agcgcaagcg
    69061 cgcactcggt gaaccgcacc cctacctgcg cgataaagtg ctggcgatgt tgttcttctt
    69121 ctcttccacc cgaacccgtg gatcgtttga agcaggcatg gcccagttgg gcggccatgg
    69181 cgctttcatt gacagcaata ccacccagat ctcacatggc gataccccca ccgagatcgg
    69241 cgaaatttat ggccgctatt ttgatggcat tgccattcgt cattgcgatt atggcgatgg
    69301 aaacaaatac ttgaacgatg tcgccaaatc aagccgcgca ccggtgctga acatgcaatg
    69361 tgatgtgtac catcccttcc agtgccttgc cgatttgatg accattatgg aaaagaaagg
    69421 caaagacctt cgcaagaaga agatcgttgt ttcctgggcg tatgccgctt cctacctcaa
    69481 gccgatctct gtgccgcaat ctttgatttt gcaaatgccg cgctttggca tggatgtgac
    69541 cctggcttac ccgcccgagt ttccgctcat gcccgatatc gttgaacagg cgaaggacga
    69601 agcaaagaag gctggaactg ctttcgaaat catcgatagc aaagacggca tgaccgaagc
    69661 ctgcaaagat gccgacgtga tctacgccaa atcatggggt ccactgctca ccactaccga
    69721 caaagtggaa ggaaaacgcc tgcaggatat gtacaagaat tggttgatgg acgatgccaa
    69781 actgaaggtc gccaaggaag gcgcgatcta catgcaccca ttgcccgccg accgtgacat
    69841 cgaagtgacc tccaacgtcc tggatggtcc gcaatctgtt gtgttcgatg aagctgaaaa
    69901 ccgcctccac gcccaaaagg ctgtgatggc tctcaccatg tcctaaaaca attacaggga
    69961 tacaacgccg ttgtatccct gtaaacagga aatttaccaa tgaccaaatt cgcagtcgtc
    70021 gccattggcg gcaatgccct gattaaagat gataaacatg tttcagtgga agaccaggaa
    70081 caagccctgc gcgaaaccgc catccacctt gtggatatga tcgaagcagg ctgggacctg
    70141 gccgttggcc atggcaatgg tccacaggtc ggttttattt tgcgtcgttc cgagatcgct
    70201 gcaaaagtgg aaggcatgca cgaagttccg ctcgatgttt gcggcgccga ttcgcaaggt
    70261 gccatcgggt atgagctcca acaggctttg cgtaatgaat ttttcaaacg caaggtcaac
    70321 aaaaaagctg ccaccatcgt cacccaggtg cgcgtcgatc aagctgaccc cgcttttcag
    70381 aaacccagca aacccatcgg ctcttttatg gacgaggcgg aagccaaacg ccgtgaaaag
    70441 gaaatgggct ggtcggttgt ggaagatgca ggccgtggct ggcggcgtgt tgtagcctct
    70501 ccgctgccga aagagatcgt tgaacttgat gcggtcaaga ccctgttgaa cgcgggacag
    70561 gttgtcatca ctgtgggcgg cggcggcatc cctgtagtag atcgtggcaa tggtgagctc
    70621 gcaggcaccg ctgcagtcat tgacaaggac tttgcttcat ccctgctggc gcaatccatt
    70681 aatgccgatc tgttcttgat cgccactgcg gtcgaaaagg ttgccattaa ttttggaaaa
    70741 cccgagcaaa aatggctcga taccatgacc gtcgctgaag ccaaagccta tcttgaagag
    70801 ggcaagcact ttgccaaagg ttccatggct ccgaagattc aggcagccat ctggtatctt
    70861 gaaaacggcg gcaaggaagc ccttatcacc aatcctgaaa atattggccg cgccctcaaa
    70921 cgcgaaactg gaacatggat cgttaaataa aatgcagggg catagcgccg ctgtgcccct
    70981 gcagccaaca tgaaacgcat tcatccttca cttaccctcc tggccgccgc agtccttgcg
    71041 tatggcttgc tcatcccaca actcggcttt tactgggatg atcttcccat gtcctggatt
    71101 cgctatcaac ttgggtttga agcgatgaca aaatattttt caaccaaccg tccgatctgg
    71161 gcgttgttgt atcaagttac cacccgcata ttgccgcatc tgccaatcta ttggcaggtc
    71221 tttgcgctgt tctggcgctg gttgggtgca gttgttctgt ggaatctcgc caaagaatta
    71281 tttccccagc gtgagaaatt tgccctcacg ctgggcctgc tttttttgtt gtatcctggt
    71341 ttcaaccaac agtgggtcag ttatctttat tctcatttct tccttgttct ttttttcttt
    71401 cttttttccc aactcctcat gctgcggggc aaaacgatcc ctgcattggt cttttccgcg
    71461 cttaatcttt ggatgatgga atatttcttc gttcttgaat tgatccgccc ctttatcatc
    71521 tggacttccc ttcgagatga agtggacccg aaagaacgga tcaaacatac cctcaacctg
    71581 tggacgccgt atctggcagt attcgccctt gcggttttat cgcgtatttt catctttaac
    71641 aatcagatct accaattcag tctgaaagac gagttggtga aagcaccgct tgcgacggtg
    71701 ttgatgctcg cgcaaaatgt cctctccagc ttttttgtgg tgacggtggc ggcctggggg
    71761 caggctttcc aatttccaaa tcccctcttg gatggtccgc ggacgatcgc ggtctatgct
    71821 tttgttttgc tggctgttgc ggcgctgacg ttgtttggtc tatggaatca ggtttcggtt
    71881 aaagagggaa gcggttcaac ttccgtgtgg cggtgggcac ttttattggg tgcggtcatg
    71941 cttgttttgg cgggcccgcc gttttggttg accaatgtcc ctgtttcgct tggttttccc
    72001 gccaatcgag ccactttgtc tttcatgctg ggtgtgagtt ttgttttggc aggtttgttc
    72061 gatttgttgc cgcaaaagtt ttacgggacg gttgttgtgc tttttgtgat gttggcagca
    72121 ggcaggcagt ttttatggtc aaacgagttc cgccgcgatt gggcatctca caagaacatg
    72181 ttttggcaga tgacatggcg tgcgcccggc cttgagaaag acacgatcgt tctaatgaat
    72241 gaagagttgt ctttttatgc cgataattca ctgggtgccg ccctcaacct gatctatgca
    72301 ccggagaatc attctgatgc gattgattat gtgttgttct atccgacaaa ccgtgaaagt
    72361 ttgtctttga gtttggatac gccggtgaat tacgattttt tggctggtca gttttcgggt
    72421 aatacctctc aaacggttgt cttttactat gcccccccca aatgtctgcg cctgcttgac
    72481 cctgagatcg atcttgaaaa ccgcttgatc cctgacgata catttttgcg tgatgccgcc
    72541 ttgctttctt caacttctcc aattctgagc aggcagactt cgcgcatgcc cgccgcctac
    72601 ggaaacgagc ctgttcatgg ctggtgctac tattttgaaa aagccgacct ggcccgtcag
    72661 atgggtgact gggaaactgt tacccaattg ggcgatgtgg cctttgccct ggacgattat
    72721 cctaatgacc cggttgagcg gtttgttttt atcgaaggct acgctcacac agggaattgg
    72781 gaaagggcgg tagaattatc cacagcttct tacaaggttt caaaagcata tgtgggccca
    72841 ttgctgtgta gattatggag ccgtattgcc cgcgaaacgg aggatacatc agagcggaaa
    72901 gtcacctttg atgtagtaca aagtgaactt gagtgttcac cgtaaaagtt tataatttgg
    72961 ttttatatgt gatcgggctg aaaggaggcc aaaggaaaaa attggtctta agtctggtgc
    73021 atagaacaaa tactaaacaa ggagaagaaa atgcgtaaaa atgtaacccg tctgtccatg
    73081 ttcgccttga tcctcatgct tgtgctttct gcatgcggtg gtggtcagtc cgccccggct
    73141 gctactgaag cacctgctgg tggcgatgcc actgaggctc ccgccgcaac tgaggcgcct
    73201 gccgcaactg aagcccccgc cgctgaagaa gctggcggct tccagatccc tgatgtggaa
    73261 gaaggcaagt tcaatgtagc gatggttttg attggcccgc acgatgatgg tggttggtcg
    73321 caggctcatt acgaaggcct cgtttacatc gaagagaacg ttcccaatgt tcacactgct
    73381 tacattgaaa acgttcctga gggtgcagat tcagagcagg tgttccgcag cctttcccgc
    73441 aagggcttca acctgatctt tggtacctcg tttggctttg gtgacccgat ggccgctgtc
    73501 gccgaggaat tccccgatgt gatgttcatt cacctcaccg gtatcaattc caatgaaacc
    73561 aactttggca acctgatggg cgccatggaa gatatgaagt acctggctgg tatgctggcc
    73621 ggtgcccgcg ccaaggctga tggcaatccc aagctcggct acatggccac cttcccgatc
    73681 cctgaagaaa ttcgccttgg taacgccatt gcattgggta tgaaaaagac ctgccctgaa
    73741 tgcacaatgg atgtccgctg gatcaatacc tggcacgacc cgatcattga aaaggaagct
    73801 gctgcttctc tgttcgatgc cggtgcccag gttgtattca ccggtgctga tacccccgcc
    73861 gttgctgatg ttgcccagga aaagggcaag tggggcgtaa cctatgactg gtacggttcc
    73921 tgcaaagtgg atgcctgtct caccgccccg tactgggtgt ggggacctgt gtactcccag
    73981 attaccgaag aagtgatcgc tggcacctac aaaccgggtt ggcagtattt tgatgctgac
    74041 actggcgccc tcggcctgta tggcttcatg gaaggccagg aaatgcagcc cggcgtgaag
    74101 gaccttgatc ctgaagtgat cgccatggtg caggaaaccc ttgcccaaat gctcgctggc
    74161 gaatttaccc gcttcgatgt gttcaccggt ccgatcatgg acaatcaggg taacgtggtt
    74221 ctgcccgaag gccagagcct gcaacaggtt gaccttgatg cgttccctga gttcggtctg
    74281 ccctgttcca ttgatgtttg tatgaagtgg tgggctgaag gcattacagc ggaacttccg
    74341 cagtagtttt gtcgatttca aatgggctgg aattctgtaa agggttccag cccttcatta
    74401 agagttttat tttcagtcgt gtttgtttta tcgcaggagc aacccagtga cacagacaac
    74461 accttccaca cctgaaatac cccttgccgt ggaaatgcgc ggcattgtca agagattccc
    74521 tggtgtatta gccagtaacc atgttgattt tgagttacgt gcaggcgaga tccatggttt
    74581 gcttggcgaa aatggcgcag ggaaaagtac tctgatgaac gtcctggctg ggctataccg
    74641 gcaggaagca ggaattatta aagtaaaggg caagcctgtg gatttttatt ccccacggga
    74701 tgccattaat catgggatcg gcatggtgca tcaacatttt atgcttgtcc cttctcaaac
    74761 tgtcactgag aatgtgcttc tggggttgga tgatccccgc ttttttatgc gcttgcctga
    74821 gtatgacgca aaagttgcgg cagtcggtga gcgttttggt ttaaaggtgg atccacgtgc
    74881 aaaaatttgg cagctttcag ttggtgagca gcaacgtgtg gaactgctca aaatgctgta
    74941 tcgcggcgcc aatgtgttga tcatggatga gcccacagcg gtacttgcgc cgcaggagat
    75001 tgacggtctt tttgatattt tgcgctcaat gattgctcag ggtaaatctg tcatattcat
    75061 cagccataaa ctcaacgagg tcagcgaaat tgccgatcat gtcactgttt tgcggaatgg
    75121 cagggtaact gcttccagtg tgccttcgaa gggtgtgagc aaacaggaat tggcgcgttt
    75181 gatggttggg cgtgatatcg tcttctttgt ggataaaaaa cctgtggagc ccggcgaaaa
    75241 ggttctggat gtgaaggatg tccacgcgga aaacgataaa ggattgccag ccctgcgcgg
    75301 actttcgctc aatgttcgca agggtgagat cgttggtgtg gctggcgttg caggtaatgg
    75361 tcaaaatgaa ctttcgcagg tcattaccgg gcttcgtaaa tgcaaaaaag gggaagtgtt
    75421 tttgggtggg gataaggtca gtaatcgtga taccttattt ggaattcaac acggcatgtc
    75481 gtatgtccct gaagatcgca ctcatgtggg gtccgccccg aatctcagcg tgaccgataa
    75541 tgtcatcatg aagaattttc gcaagccgcc catttcaaaa ggtggattgc tggatatgac
    75601 cgccgcaggg aaatttgcca atgagttgaa gcaggcttat gacattattg tcccgaacgt
    75661 aaatacgcct gtacgccttt tgtcgggtgg aaatttacag cgtgtcatcc ttgcgcgtga
    75721 aatttcagag cacccggatt tcatggttgc cgttcagccg acgcgtggtt tggatgttgg
    75781 cgccatcgaa ggtgtgcata aattgttgct ggctcaacgt gaggcggggg cggcagtgtt
    75841 gttgatttct gaagagttgg acgaattact cggcttatgt gaccgtgtgt atgttattta
    75901 tgaagggaag atcatgggtg aagtgcccat tcccatcggc aagcgtgaag aggatctgat
    75961 cgaaaccatc ggtctgatga tgaccggcac cccgttggac caaatccaaa aagaaggagt
    76021 cggcgcaaat gactgagaca acttcaccac aaaagaaatc catgggcttc aaaattcgag
    76081 ttgaaccgcg actggatgag ccacctgcct ggcaccctgt gcttgtgtcc ttgggcgcga
    76141 tcattgtggc gcttattttg ggcggtattc tgatttcttt tgcaggcggc gaccccatta
    76201 aatcttattt gcatatcgcg gatgcgtctt ttggcgacac cggggtttta tcagacacca
    76261 ttgtgaaggc taccccaatt ttgctggccg ccctggcttg ttcggtggct ttccgcatga
    76321 agctgtggaa tattggtgcc gagggtcagt tcatcatggg agcatttggg gcaagcgcag
    76381 ttgtccttgc tcctttgttg gcggaggata ccccacgttg gttgtttctg actgtgatgg
    76441 cgctggcagg catggctgca ggtgcgattt gggggtttat ccccggatac ctcaaggcca
    76501 aatttaacgt caatgagatt atcagcacct tgatgatgaa ttacatcgct gtggcttgga
    76561 tcaatttttg gatctttggg gtatggtctg aaggtggttt tcagatgtca ccgaagttcc
    76621 caaataatgc atggcttccg cgtctgctgg agtatgcgaa gacctacccc gccctgcgtg
    76681 gactaacgac tcatgcgggc cttttgttgg ggatcgtcgc ggcggttgtt ctctggctga
    76741 tcgtgtatcg cagtcgctgg gggtatgaaa ttcgtctcat tggtgataat ccacatgcgg
    76801 caaaatatgc gggtatcaat atcacaggga acataattta cgtgatgatg ctttccgggg
    76861 cgttggctgg gttgggcggt atgagtgaag tttcgggcgt ggtgcatcgc ttgcagactt
    76921 cgccgttggc tgcgggatat ggcttcacag gaattattgt ggcatggctg gcgaaattaa
    76981 acccgatcgt cattgttttg gtatcagtcc tttttggcgc gttgattttg gctgggcgcg
    77041 agattcagcc atcgggtgtc ccaaaaatga tccagggcat tattctggtt tcactgattg
    77101 ccagtgactt tttactgcgg tatcgtatcc gcatcgtgcg cgcagacgcg gaggaataat
    77161 tcaattatgg atccaatcat catacttcag gcaggtgtgg ccaccggcac ggttttgttg
    77221 tttgccacca ttggtgagat ctttgccgaa cgttccggcg ttttgaacct tggtgtggaa
    77281 ggcatgatgc tgatcggtgc aatgaccgca ttcagcacca ccatcgcaac agggaatcca
    77341 tggcttggag tgttggcggc catgctggct gctggcttga tcagtcagat tcatgctttt
    77401 atcacgatca ctttgcaagc cgatcaagtt gtcagtggct tggcgctcac ttttgtagga
    77461 acaggtatca gtttggtgtt aggcgaggga ttgagcaagg cgggcacggt ttctctcatg
    77521 ccgaattttt cgatcccgct gctttcccaa attccgtttt tgggtcccat cctgtttaca
    77581 aaacaaagtg tgctggttta catcggttat ttgctggtgc cattgacctg gtattacatc
    77641 aaccatacgc gccctgggct gaatttgcgt gcagtgggtg aatacccctc cgccgccgac
    77701 tctttgggta ttagcgtgtt ccgcctgcgc tatttttacg ttttcattgg cggaatgatg
    77761 gctggtttgg ctggagccac catcagcctt gcagtggccc cgggctggtt cagtgaactg
    77821 accacggctg ggcagggatg gatcgccgtc gggttggtca tttttgcgca atgggatcca
    77881 gtccgcgcga tgtttggttc gtatgccttt ggggcgctcc gtcgcttaat tctcgatatt
    77941 caaggtccaa ccattctctt tgggttcaac aatccttttt actacaaccc gtattggggc
    78001 tttttcctgc aaatgcttcc gtatgccttt acagttattg tattggtgat tggttcgcgc
    78061 gaagccattc gcaaacgttt gggggcgcct gcagcgcttg ggaatcctta tgtccgcggt
    78121 gagcgcggca agtgacgagt ttctgacaca gctttgattt ttaaatcccg cagggaaatg
    78181 cctgcgggat ttttttgcat tgccgtacgt ttggcgagaa atactggaca aatgtcactt
    78241 gtgacaggat gctcaaagta tccataatgg aaaggtcaga cgattgtcat ccaacgactt
    78301 ttctcttatg agcatcattc agaccaaatc ggtggcagac caggttgaag aaattcttcg
    78361 ggaacgcatt cgagatgcga cgtatttgcc cggcagccga atgccttctg agagtgagtt
    78421 atctgatgaa atgggggtca gccgtgccac tgtgcggacc gttctggcaa aattggcggt
    78481 caatggtttg atcattcgga agcaggggga tggaacgtat gtcaatgcgc gggcacagga
    78541 agccagtgcc cataccggga atttatggga tttggtccgc ctgatcgaaa acaacggctc
    78601 gaagccgttc attcagccga tctcaattac ccgtaaacct gcttctgaaa aagaggcaca
    78661 ggccctcgcc gttgaagctg gagaacccct tttactgttc aagcgtttat tccttgctga
    78721 ccaaattccg gtcatttatt caaacaacct tgttccgcta tctttttttc gtgtatccac
    78781 tgatgaaatt gacgggcaat tacacttccg cgatattctg cgccaatact gtcatcagga
    78841 aattggattt gtgatcaccg atattcgctc cacactcgtt aatgatgaat tgcggcatct
    78901 gttgagaagc gaacttggcc ccactgtcct tgagttgcaa atgacgtttt acagccgaaa
    78961 tagccagcca ttggcgctgg gattgaatta cttcgatgat accgccctgc ggttgggttt
    79021 ggttcaggca tggaattaat tcctgaatca aaatatttga atctgatgtg gcgctgagga
    79081 ggaaggttcg atgcgatcaa gattgaacgc tttgttcgat gctttgctgc cagtgcttgc
    79141 caccctggct gcgcttgggg tgggggcgat catgttgatt ttcctcaagg tcaacccggt
    79201 gacggcctat aaggcgcttt gggacggggc ttttggcagt tccaatgcct ttgccgaaac
    79261 tttggtaaag gctacaccat tgttgctggt ggcgttggga atttgcatct catttcgcgg
    79321 tgatgtgatc aatatcggcg gcgaagggca aatgatcatc ggcgcggttt tggccacatg
    79381 ggtgggtctg acgtttactg gattgcccgg ctggttggcc gtgactcttg ctttggtggc
    79441 tggttttctg ggtggggcaa tttggggcgg catcccgggg gtgttgaagg catacttcag
    79501 tgtcaatgaa atcttaagca ccgtgatgat gaatgccatt gccgtgcagt tgatgaattt
    79561 ccttttgcgc ggcccgatga tcgacccctc gcaggcggaa ctggcttcaa aaattccgca
    79621 gacggcgcgt ttgttggatg cattccgctt gccgcgtttg gcccccaccc gattgcacct
    79681 tggggctctc attgctgttg tgttggcggt tgctgtttat attttgctct ggcggacaac
    79741 ccttgggtat cgcatccggg cagtggggca aaacccaaac gcttctcgat atgcagggat
    79801 caaagtgcag cgttacatgg tcttggcgct tctgcttagc ggggcatttg cagggttggc
    79861 tggggctgtg caagtttatg gtgtgaacta ccgcatgatc accgatggtt cttcttcagg
    79921 gtttaccggg tcggcggggt ttaacggcat tgtagcggcg ctgtttgggc aactgcatcc
    79981 gctttggtcc attcctgcgt ccattttatt tggggctttg ctggtagggg cgaacaagat
    80041 gcaaagagtg gttcaggttc cttccgcact ggtcattgct ttgaacggtt tggtggtggt
    80101 ctttgtagta agcagtgaga tcctccgcaa gagacgccag cgcagaaggc tcgcagatga
    80161 tgaaacgccg cccgtggcgg ggtcggctgc aaatcaatcc acacaagcgg agacaggcac
    80221 atgatttcag atctctttac cgtcactgtt ctcattggca ttctcgcttc tggaattcgc
    80281 cttgcaacgc cgtatctgta tgctgccatc ggcgaaacct tcagccagaa gagcggcgtg
    80341 ttgaatttgg gcgtggaagg gcagatgctg ctcggtgcct ttgccgcttt ttatgtttca
    80401 ctcactacag ggaatttatg gctgggtttg ctgactgcca tgttggtggg ggcattaatg
    80461 gggttggcga tggcctatgt tacggtcaac ttgcatgcgc agcagggcat cagcggcatt
    80521 gggttttatc tcttcggttt gggcatgagt gacctgctct tccaaaagtt gatgggcacc
    80581 gtggaaacgg tcaaaggttt tcctgaaatc tatattccgg gcttgagcga tatccccggt
    80641 attggcgaaa tatttttcag ccagaacatt ttggtgtatg gcgcttatct gttggtgccg
    80701 ctcgcgtggt tcgtcttgaa caagaccaca ttggggttaa agatccgttc ggtgggtgag
    80761 aatcccgatg ctgccgattc gttgggggtg agtgtggcgc gtgttcgtta cttcaccatc
    80821 attcttggcg gtgtgctttc gggcattgct ggcgcgtcgc tttcgatcgc gttgttgaat
    80881 gtattccagc aaaacatgac cagcgggctg ggcttcatcg ccgtggcgtt ggtgtatttt
    80941 ggcggatggc ggcctgtggg ggtgctgggc ggtgccctgc ttttcagttt gatcaattca
    81001 ctgcaattgt ggattcaagt tttggggata cccatcccct ctgatattgc ggtgatgatg
    81061 ccgtacgttc tgacgatcct cgtgctggtt gccacggtat cgagagtccg ctctccttcg
    81121 gctttgtcta aaccctttga aagggaaaat taatttcagt ggataggagg caatatttgt
    81181 ccctgtgaaa atgtttttcg aaagcgtatc cgttgtcccg tttggaaaga agcgtgttca
    81241 ttcatccata aacaaggaga agaagatgaa aaaatttgca ctgtctctcg taatcgcttt
    81301 tgtgttgttg ttgagcgcat gtggcactcc tgctgccact gaagcccctg ttgttgaggc
    81361 ccctgccact gatgctcccg ctgaagtgcc cgccgccact gaaccgcctg ccgcccccgc
    81421 cgaacctttc cgtgtggctg tggtgatgcc gagcgccacc aatgacctgg ctttcagcca
    81481 gagcatgtat gatgcccttg tgcgcattca ggaagaaatg ggcggccccg aagcctttga
    81541 gttcgttttt tcggaaggca tgttcgttgt agatgatgcc gctgcggcca tccgtgacta
    81601 tgccacacag ggatatgacc tggtgatcgc acacggttca cagtacggtt catccttgca
    81661 ggaaattgcg cctgattttc ccgagaccag ttttgcgtgg ggcaccacgg ctgatacgtt
    81721 tggtcagccc aatatctttg cctatgaagc ggcttctcag gaaggcggct atgtaaatgg
    81781 cgtattggct gccactcttt cgcagagcgc ggtgatcggc gttgttggcc cgatcgagac
    81841 cggggatgcg aagttgtatg tggatggttt caaggctggt gttgcagcca ccaaccccgc
    81901 agtgaccgtg aatgtgaatt acatcggttc cttctctgat gtagcgttgg cttccgaagc
    81961 agccaccact catatttctg ctggtgctga tgtgatgacc ggtaccgccc agatggttgt
    82021 gggtgcaatt ggcaaagctg aagaaaataa tgttttgtgg ttcggtacgc aatccaacca
    82081 gtcttcactg gctccgaaga tcgtggttgc cagtcaggtg tatcactggg aagtgaccct
    82141 gcgtgagatc attgccctga tcaaggaagg tacactgggc ggcacttcct tcaaggccaa
    82201 tcttgccaac ggcggcgagg tgatcgagtt caaccccgat tatgcgcttt ccgatgaggc
    82261 caaggccctt gctgaaacca ccattcaggg aattattgac ggcaccatca agatcacgct
    82321 gccataatag tttaataccc aggcctgaca ggtttctgtg aagcgacctg tcaggcctcc
    82381 atgagaaata attcaggatg gcacccatga ctgaatcaac caaacttcct tccggtaaac
    82441 cccgcattga gaccatcgaa atgcgcggaa tcaccaaacg ttttcccggt gtgatcgcca
    82501 atgacaaggc cgatttcgat attcacgcgg gggaagtcca tgctttgctg ggagaaaacg
    82561 gcgcggggaa aagcacgttg atgaagattt tgtacggcat gtatcgcccg gacgaaggcg
    82621 agattttgct gaacggaaaa ccagtgacca ttgcctcgcc aaacgattcg atcaacctcg
    82681 gtattggcat gatccaccag cacttcatgc ttgtgccaac cctgaccgtg gcggagaatg
    82741 tggcgcttgg gttgccatcc actcgtgggg cgctgactga tttagaccgt gtttcaaagc
    82801 ggatcgttga actggctggc atctatggtt tgaagattga tccctctgct tatgtctggc
    82861 agctttctgt tgggcagcag cagcgtgttg aaattatcaa agctctttat cgcggcgcgg
    82921 cgctgctcat cctcgatgag ccgaccgccg tcctcacccc gcaggaggtg gacgagttat
    82981 tcgtcatcat gcgccagatg gtgaaggatg gtcatgcgct gatttttatt tctcataaat
    83041 tgcacgaggt ggttgagatc agcaaccgca tcacggtgct gcgtgatggc cgcagaattg
    83101 gcacacgccc aacctccgaa acgaccaaac aagaccttgc caattggatg gttggccgtg
    83161 aagttggttt tacgccagac cgcggcaagg tggagatcgg ggaagcccgt ttgaaacttg
    83221 aaaaggtgga atgtggcagt gaccgtggga cacccggtct gcgaggggtc agcattgaag
    83281 tgcgttcggg tgaaattctg ggtattgcgg gtgtttcggg caacggtcag cgcgagttgg
    83341 cagaagcgat caccggtctg cgcaaagtta ctgcaggcag ggttttcctt gaggggaaag
    83401 acatcaccaa ttttgccccc ggtgatatta ccgagctaaa tctatcctat atccctgaag
    83461 aacgcatgcg cgatggcatg atcaaagaat tcaccattgc ggagaatatg atcttgcgcg
    83521 agcaccacaa aatgccgtat tcacgcatgg gctttttgca tttgggtgat atttccaaac
    83581 atgccgatga gttgatcgca aagtttcatg tcaaaacccc gtcacgtgag acccatgcca
    83641 aaaatctctc gggtggaaat attcaaaagg ttgtgcttgc acgcgaaatt tcacgcaacc
    83701 cgcgcgccat catcgcagcc caacccacac gcggacttga catcggcgca actgaatatg
    83761 tccgcgaaca gttgctggtt gaacggcgca aaggtgtggc ggtcatgctc atctccgaag
    83821 accttgatga aattctcgct ctttctgatc gtatcgccgt gatttacgaa ggccaggtga
    83881 tggatattgt tccgcgtgcc gaagccaccc ccgaaaggtt ggggttgctc atggcgggcg
    83941 tccaccccga ggttggttag ctcgtgtcgc ttcctgctca agccgccggg agcatttatt
    84001 cttattgatc aactgtaact tgatgtaatg catccaacgg tatacgaaag tgttcatcaa
    84061 gtagtaaaat cgaattccat tggtcgggtg gtcgctgatg tgaaatgcat cagcggcgtt
    84121 gggtcgctca gcgcccatcg aaaagtaccg ccgccttagt agaaaggaca ccacctatga
    84181 acctttggca ggaatacaaa cgccccgcct ccgtaacgga ggccattcaa gcacttacat
    84241 ctgcatctgg ccctgcaatg cccattgcag gcggcacaga tctattgctt gatttgaaac
    84301 agggacgcca tgcccccatt cacactttaa ttgacctgac cttcgtcacg gaaatgaccg
    84361 ctctcgaatt aagagggaat gaactcttta ttggagctgc cgtcccagtc aaccgcgtcg
    84421 cactggaccc gctaacgggt acccacgccc aagccttggt cgaagcctgt aatttgatcg
    84481 caggcccgca agtccgcaat actgccacgc tcggcggcaa cgttgcccac gctctccccg
    84541 ccgcagatgg aacgatcgca ttgaccgcgc tcaacgcgca agctgaagtg gctggccttt
    84601 caggtacccg tcgaatgcca ttcacatcat tgttccttgg ccctggaaaa tctgccatcg
    84661 ataaaacaaa agaaatcatt gtcggtttcc acttgccact ttccactccc cactctgcct
    84721 cctgcttcaa gcgcatcatg cgcccgcagg gggttgcgct tcctattttg aattgctctg
    84781 tttggctcga aagggatgcg gaaatggtca aggatgtaca cattgctgtc ggcccgggca
    84841 gttccgttcc tttccgcgcc acagacgccg agaacgctct gcgtggaaaa tccctcaacg
    84901 aagaaacctt caatcaaacc ttggatgccc tgctcgggca ggctcaattc cgcaccagtg
    84961 caagacgcgc cacggcagat taccgcaagc atatcgtcgg cggcttgttc aaagatgtgc
    85021 tcgatactgc atgggccaga acaagctaac cactgagaca cggagacact gaggttttaa
    85081 aggattaatt tatgcaacag cgtaattttc ctgttttgca aaacaaacat gcagtcccgc
    85141 ttttggaaaa agacataact gataagatca taggtgctgc tattgaagtt catcgtgcct
    85201 tggggccagg attattggaa tcggcttatc aattatgtct tgcacaggaa tgtaggttgc
    85261 agaaactttc ttttgaagaa cagattgcgc ttaagctaaa ttacaagggc gtagaaatgg
    85321 atggcggata tcgaattgac tttgtctttg acaagcgtgt tgttgttgaa ctcaaagcag
    85381 tcgaagaggt gctgccagtt catgaggcgc aattgcttac atacttgaag cttgcaggaa
    85441 ttcgtgtcgg cttgttgatc aattttaata agcctgtctt gaaagatggc atttaccgac
    85501 gggtcctttg accataaacg gaataaaata aaaaactccg tgtctcagtg tctccgtggt
    85561 tcaatttttt gaggaaaact atgaacaatc aaatttcact tatcgttaat ggcaaacaac
    85621 atatcatcga agcagttgct ggcgagactt tatcaacttt gctgcgcgag agacttcgcc
    85681 tgactggaac caagatcggc tgtgaagaag ccgagtgcgg ggcttgtacc gtcctggtcg
    85741 atggacagcc gatgatgtcg tgtgtctatc ccgcggagcg tgcggacggt aaggtcgtca
    85801 ccacgatcga aggattggcg cagcgtgtcc acgaagaaat gaaactgcat ccgttgcagg
    85861 aagcctttgt ggagcatggc gctgtccaat gcggattctg catccccgga cagatcatga
    85921 ccgcgtatgc cttgctcaag cgcaatccga atccgaacag cgatgacatt cgctttgctt
    85981 tgaaggacac cctttgccgt tgtgcaggct atccatccat tgaaaatgca atcattgctg
    86041 cggcgcaggc attgcgcaca ggcgagcctg tccaaaagcc gacgc
//
LOCUS       DAOQLC010000004        80274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_55_length_80274_cov_22.355602, whole genome shotgun sequence.
ACCESSION   DAOQLC010000004 DAOQLC010000000
VERSION     DAOQLC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 80274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55_length_80274_cov_22.355602"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..688
                     /locus_tag="PL000_01540"
     CDS             5..688
                     /locus_tag="PL000_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC87604.1"
                     /translation="MTKILIVDDEPSIVNLVQAYLKPEGYEVFTAVDGPSGLKAARAF
                     KPDLIVLDVMLPGMDGLEVLSRLRRESQAYVILLTARTEETDKIVGLSMGADDYVTKP
                     FSPRELVARVKAAIRRIQTGTGPSAEGSVLAFRHVRIDTGARQVTVDDQTIDLTASEF
                     DLLHALAENRGRVLTREQLLERVWGGSYFGELRVVDVHLGHVRQKLGLPDVISTVRGI
                     GYRFDDETA"
     gene            704..1864
                     /locus_tag="PL000_01545"
     CDS             704..1864
                     /locus_tag="PL000_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87605.1"
                     /translation="MKILRSYLGIKLFLSYFVVILVGMTVIGITTKIVTPGAFARHLL
                     AMENQIEMGQGMGQGMGQGMGQGQGQGGGMMSEFYRSFQATFTEALLIAVVAASLVAL
                     VVSFVFSRSILSPVYVMMTASQRIAEGRYDERVQLQGTDELNQLAGSFNKMAEQLEQV
                     ENMRRRLLGDVAHELRTPLTAIKGSAEALMDGILPASDETYQQIHAEAERLSRLVDDL
                     QELSRVESRAIQLDLRAVDATVLIQTIAKRLQYQYDEKRINLTLNLPADPIHVLADEG
                     RAIQVLTNLAGNALQYTPENGSVTISVKKEKDEAQFSVYDTGAGIPAEHLAHIFDRFY
                     RVDKSRSRAHGGSGIGLTIARHLVESHGGRIWVESAGKNKGSVFSFTLPLAK"
     gene            complement(1874..2521)
                     /locus_tag="PL000_01550"
     CDS             complement(1874..2521)
                     /locus_tag="PL000_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87606.1"
                     /translation="MMKRILTLLFLALTLSSCDTWGNLPQPLPVWTPVPSQLPNIITA
                     TPIILTPGVIVLTPVTSTATNTETPSPVPTFTFTPFTPTETPVTPIQALTVTILGCNT
                     SIDILHGMGEVTNAYVTVTNTGTTDLPNTCALLRAIDEDRDHPDKKVCVPNLPIQNQV
                     TLKLTVDSAYNKDTIIQVDASSNDLLLLRVDRQSCTDISLFGGTPSDIGVVKPIQ"
     gene            complement(2613..2894)
                     /locus_tag="PL000_01555"
     CDS             complement(2613..2894)
                     /locus_tag="PL000_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNC87607.1"
                     /translation="MSNDLYNKTPSQIVMYSTEYCPDCHRAKKFFETNGIEYLKVGLE
                     NNEEATLFVMDINDGNRSVPTIIFPDGSVLVEPSWEELRIKTAIPTETL"
     gene            3180..4040
                     /locus_tag="PL000_01560"
     CDS             3180..4040
                     /locus_tag="PL000_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87608.1"
                     /translation="MTNLKRSVLSIAFFLFGILVLGQLDRSDTPIINFASYFYLLAIF
                     IVPVMVFVPSLHKISVVVPMVFWGAVYFGIFQLTDRGNTGSLDVEVVVLELVIIEVGV
                     WLSFQLALGIERSESLMDVLAQGTFPHRALEIDLANEQIKVEFSRSRRYHRPLSLLVL
                     HAFPQDEAVVREMLMSLQRDVLTRLSNARVGQAIGESIRQTDLLVRDKVGRYIVLCPE
                     TDLESVYLLADRLSKIVVERTGLHVNCGVASFPDEALTFEDLLHLARDRSKQPLPSKA
                     AIEMKEQAVK"
     gene            4037..4720
                     /locus_tag="PL000_01565"
     CDS             4037..4720
                     /locus_tag="PL000_01565"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC87609.1"
                     /translation="MSSTSINPADQYSWIASFDPKKRIFVGRSYFILKRIMDLSLVLL
                     SAPFWAPLLLVLGIIIKVTSPGAPALFTQFRTGKNGKRFSMYKFRSMVPNAEALKEKY
                     AHLNELQWPDFKITNDPRITKIGKILRKTSLDELPQLINVLKGDMSLVGPRPTSFGAE
                     TYKLWHTERLDVTPGLTGLWQIIGRASMEFDDRLRLDIAYIERRSIWMDINILFFTVF
                     AVFKARGAH"
     gene            4930..6015
                     /locus_tag="PL000_01570"
     CDS             4930..6015
                     /locus_tag="PL000_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87610.1"
                     /translation="MKRIPFFIILACLLFTSCTHGSESTPVSSELGGDFDNGGDVVSH
                     PNSSENADVDCDLPYTKDSIWNIPIDWSIAKIHPDSDKMMEAFWDGSRWIGSDPTQYA
                     PNIYFVDNKTPLVPVKLRKNRFRDAFDDKEIQYGEPAASVWMPIPEGAQPAPGTDGQM
                     VVINVDTGEEWGLNKGTIDPLGSWFANGIYRYSIENSGVPPEGFGQRGAGIGNFSGIV
                     RKCEVDLGVIEHAVTLAYDFPCTPETCGANGRPAFIPPFTKTDGRGTSTYDIPEGARM
                     IIHPEITKEEIDNACSGMKGCIVWVLAMQKYGGFIVDNSNHPKTYPEGEATANWDPEI
                     WSDDMLRNIPTEWYDILDWNYPSTTIK"
     gene            6043..7533
                     /locus_tag="PL000_01575"
     CDS             6043..7533
                     /locus_tag="PL000_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC87611.1"
                     /translation="MITRLKKIEKSDVVFFVLSIVLAAGSALAIGNLDSDIGLYGLVG
                     LVGIAVALTMFVMPSFGANVLIIAVFSNVSDELSNNGYPGIIKPLVVVVVATIVARFI
                     YFEKPPAARSTTTRIEAFLFLYFLVYAFSFMMAADKSRASSVIFDVAKDIVVIYCLMY
                     TLRSPEALKRAVWVVIVVTFILCLLGVYQSITGNYSNNFFGFAMVSLDDFSGSTAARL
                     GGPINAPNMWGQTVVSVIALVAFRIIHERRMTTRLFSMLVLGVFLFEVLNTYSRGDYL
                     ALAVVVVLIFFVFEDKFSPMIAFAGVGLIILLIPFIPSSYTERFASLSILTSGDQSGI
                     YQEDSFRGRSSELLTGLSMFKASPFLGVGAGNYKYNYQKYSQLIGIEQRAEARDPHSL
                     YVQLLAENGILGFLAFVAVIVSVFSGLNKVKRSIDYLPALRESWVPWISSLQTSMVGY
                     LVAATFLHGAYIRYFWILVGLSLTAIRLTDEMVAETNQAALSKTTV"
     gene            7679..8461
                     /locus_tag="PL000_01580"
     CDS             7679..8461
                     /locus_tag="PL000_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87612.1"
                     /translation="MDAKRMMSKLRSLPALVYENFLVRSYHNLFGKQINDRFVILGHA
                     RTGSNYLFDGLISSKSITGYHEIFADHNRAIGKDFEKVLSQVYRKESKDTQLVGFKLF
                     YNHLTDEEWAKFLQHEEFRIIHLTRANRLRTIVSLDIAFKTDEWTRSSPSKGNQLDKK
                     RITLDTSKLIGRLEKIQAQEELARKRFAGRPMLEVVYEELVRIPKVVFKQAEDFLGVN
                     DIDFTKIKIMKQNTEKLEQLIVNYDEVSQCLKNSQFASCMDE"
     gene            8532..10010
                     /locus_tag="PL000_01585"
     CDS             8532..10010
                     /locus_tag="PL000_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HNC87613.1"
                     /translation="MTDESVKQDAGSQFSRKVAGGLLWNSLTFGFGKLVVLLTTSVLA
                     RLLTKAEFGQVAVAVVAINYLAVIKDLGLGVALIQRRGEVDNAANTVFTINTMLGFFI
                     SALIIPLAPPIADYFRDPGVVPVLRWLGLSFAINALGSVHMIWLLRDLDYRRKFIPDM
                     GNTVVKGIASIGLAFAGFGVWAMVIGQIAGALTSVILVWIILPWRPRLMIDRSIAGSL
                     IKFGSSIIGGDILGTIIDNIDYIIVGRLFGLAQLSVYTLAYRLPEMLLIGNLWVLGEI
                     AFPAFSSIQDKPEEMRNGFLGSVRLVELIATPISLGLFIAADPIIRVVFGEQWLEAIP
                     MLRVLAIYAWVYSIGFHIGGIYKAVGRPYILFNLSVFTLIILVPALLIGAQFGVIGIA
                     WGHLIAVLIRRVVSLAVATRFVNVTLKDIFSQLVPSLRAGLVMTPLAFAVLYAASGLT
                     PIVQLILVVITGAVSYLGVAWWTEKENLFRLMKAIGGSKKKA"
     gene            10023..10814
                     /locus_tag="PL000_01590"
     CDS             10023..10814
                     /locus_tag="PL000_01590"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC87614.1"
                     /translation="MQNHRFIFVSGLHRSGTSILFKSLRDHPEVSGFHNTNSPEDEGM
                     HLQTVYKPSGYYGGAGKFGFNPEAHLTEASPLVTEENRKKLFAEWTPYWDMSKQYLLE
                     KSPPNLIRTRFLQAMFPDSYFIVMMRHPLAVSYATRAWYKKYRIYWRRLPRIFEHWLV
                     CHEIFLEDKKHLKNAMLLNYEQFVADPSTYTNKIYEFLGLSPFPNNQKVLSSVNKKYF
                     SMWEKDQKGFLSGFIARRMISRFETRLNRFGYSLLDVERADALAK"
     gene            10817..11476
                     /locus_tag="PL000_01595"
     CDS             10817..11476
                     /locus_tag="PL000_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87615.1"
                     /translation="MNSQSSPSTAAHISLPSHGVDWRFLLPLREHTRMLVIHPSDADY
                     DRSFQEFGIPFETVSFDNFNTDRSPLQFESGSFDIVAAPLGFGGDNRAGRQSQLGKNL
                     LAVRSLIRAGGMFLLGFSNRWDIRRKGREFPNSSTTWRISRSLERAGFDGMRFYGTFP
                     EPLTPEYIFPLNSQSLAFVLNYRYQHKLPGYFLKLTRTPAVNMLLGFVPFYYAVASVE
                     A"
     gene            11476..12492
                     /locus_tag="PL000_01600"
     CDS             11476..12492
                     /locus_tag="PL000_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC87616.1"
                     /translation="MAALIDDLTTLLVSNSRRSINSNLNWVMLSNGAADPNDNVIFFG
                     FEGKATVPSFVAKVPRLPSNGRILREEYDRLAEVWGLLGKDASLLVPEPLAFFDVGQQ
                     QVSVISFVRGDGLLFSSKKKLWHDPIQVLNLSKTVAGALRELHEKTAKEAADVVSIPT
                     DFLENLELFKKIYTPTAEESLAFSELEQYFARHSVHYQTLLHGDFWHGNIIRSSQHGT
                     LIFVDWQHSRWSSDVSADVYMFLMAGALSASRGSPEERASLTAKLLVNWQQEIFPAYL
                     AAYGPTERYSLLPPRLGMQVCCIEKAVRSIRDFGYNQFDDLIWRNLLTELVNIPKDGF
                     LDGS"
     gene            12482..13651
                     /locus_tag="PL000_01605"
     CDS             12482..13651
                     /locus_tag="PL000_01605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC87617.1"
                     /translation="MAANISAKRKYNIIFVIDGLGMGGAERLMVPILKNLSREYFEPR
                     VCVLHVKDGNPIAEDLRALGVPVDLLLIPYLRDVTAIPRLRQYLRDVKADLVHTQLEM
                     ADIMGNLAAKSMRLPSVSTMHTMPSQDMKLKSKLHQTVELLALRHFCNKVISVSEEAR
                     KFYLKISNLLPQNLLTIYNGIDLSHFEHLNHQQDRVKIREEFGLPAQAKILITVAVLR
                     EFKGIQYMIRALPAVVAKDPDVYYLVVGNGTYREALEKEVEQAGMKDHVVFAGQRSDI
                     PHLLAASDVFVLPTLTEALPTVLAEAMGASLPIIASSVGGVPEMINEGENGLLLSAGD
                     VDGLISASASLMADEGRRKAMGERGRNIVNEKFNIHVQVERLRDLYLGLIKEYEK"
     gene            13641..14900
                     /locus_tag="PL000_01610"
     CDS             13641..14900
                     /locus_tag="PL000_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC87618.1"
                     /translation="MKNKLRIFVVEPNNGGGLVHFAYQLCNALASEGADVTLVAGAKY
                     EMADQPHSFHVEKLLNLWEHFDPDSMKAEIQNPLKRAWRKIYWTLRRMARGVRLIYAW
                     ASLTQFLLREKPDIVQFSKYEYSFESYFVGYLRRRGLIVTQLCHEFELRESQDFIWSI
                     LDRISGEVYRHFSAIFFLSEEIRKRFLSLHSTIPAERTLSVPHGNSGWLLNIPAVSAD
                     VLRQRYGLQKDERVVLFFGLLAPSKGLDDLLDAFAIARKTCNAKLVIAGYPTKHISMS
                     EMLEKNRNLGIADQVIFDARYIPLNEIQPLMALASVVVYPYRSGTQSGALQTAYTFGK
                     PVIATAVGGLPEVVENGKSGYIVSPNSPAELAEKIVAVVNDPKLGDEMGQYARHLSET
                     RFSWKTVAVQIVEVYTRLLEARKRVSN"
     gene            complement(14897..15949)
                     /locus_tag="PL000_01615"
     CDS             complement(14897..15949)
                     /locus_tag="PL000_01615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC87619.1"
                     /translation="MYSDKTNPRKFSRSGLMKVFTDAAAATGLIRIGRQFWARSLTVL
                     NYHRIDDPHRKDFDSFQPNISAHPAEFDRQMEYLSRWFNVVSLQDVVTWLTGGKPLPP
                     YAALITFDDGYLDNYTNAYPVLKKYKFPAVIYLTSGHIDTNEPFYWDLAAYCFLNTKT
                     DHVLFPDKKELFWKNPIERWQIAKSWIYAMKPLPEEEKRKWVNQLPEQLGVSIPDNFF
                     RNLMMNWDQVREMANNGIDFGGHTVNHPILTRISLEQARSEIETSKAKIEKELGRPIL
                     SFAYPNGMQTDYNTDIQKIVADAGYKVAFTLQNGPTTLQEVQQSPFTIRRVFISHAHS
                     LSQYATITSRFNRLRN"
     gene            complement(15980..17134)
                     /locus_tag="PL000_01620"
     CDS             complement(15980..17134)
                     /locus_tag="PL000_01620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC87620.1"
                     /translation="METCRPLIIDLAVGYGGSSSRVLGILRALADQGAALAGLDQGAV
                     VLAAQKEGLPVYTVGAKKTDPRIVSRLVRLIREKDFQVLDTQNIQSKFWGSLAAGITN
                     TSLVSTINSWYASEHGQSSGKDRLKGRMYTALELFTNRNLDLYITVSEKDRQALMKSG
                     IPSQSIELIYNAVSFDDSQISSDPDGLRKKFNLPPQSLICTAVGRLVPVKGYDLLIEA
                     VRQVAAQIPQLYCLIVGEGECMEELSNQIKDAGLQDRVLLTGYQDRNNVMPILKSSDI
                     FAMPSRYEGTPIALLEAAAMGLPIIAAAAGGIPELVTNQEHALLVEPNNPAALAEGLR
                     KLCSDLKFAQKLGDNAQQRVRTVFNLDTQIQNTFHAYQKALDRHDAKRKK"
     gene            complement(17138..18340)
                     /locus_tag="PL000_01625"
     CDS             complement(17138..18340)
                     /locus_tag="PL000_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC87621.1"
                     /translation="MFSLAFILVILLVISLGLLLYIYVGYPLIIVVLSKYRRQPIVKT
                     GTLPTATIVIPAHNEEAVINQKLDNILSLDYPRELLQIIVCDDASDDRTAEIIRTYAS
                     KGIQLSEGARAGKVGALNRALGLATGQIYVITDADTILNQNSLRELLVNFSDEKVGCV
                     VAQTRMMSSNEQETSESGGLYWRYEALIRQKESDLHSTVAATGHLMALRREIMQPIPS
                     NVILDDFYLAMMTISQGYRVISEPKSVVWERPTNTMGDDINRRRRLTAGRYQMVFMSR
                     EYLPKLSSLLRFQVISHKFLRLAIPHLMILALLTNILLVALLPALSLSPLANAMLIAL
                     AILQGIFYGLALLGAVLSNTPLKKSKVFKIFMLPYFLCATNFASIAGLLWYFSGQHTV
                     LWQQAKRQ"
     gene            18494..19606
                     /locus_tag="PL000_01630"
     CDS             18494..19606
                     /locus_tag="PL000_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNC87622.1"
                     /translation="MEIRFYFKIIQRGWWIILVTTLVAINVSLVYSYYFTTPMYEAVA
                     RFIVSPNMQNTESRDLVNSLEALDKRSIISTYAEVLNSHQIIESTMELLRESPNDFRG
                     YSTSVTVVPDANIIRFSVKGPDPDVAALLANSIGQHAIDYVKNLYVIYDIDFLDKALV
                     SGDPYQPQPAQDAGLALMVGLVLGVGLAIFKDQITGTIGKLGERNMLDVESTAFTRAY
                     FERRMRQEVINQPSSVLTFGVIHLLGIQEIYDSLPQAYVNQILRKVTETLKYQLRGND
                     IVGRWSQLEFSILLPSTDGVSAMNRLERIRELLNQPISLEGESGIDIVLDPRIGFADR
                     QGGESINVLISQVEKALELSMESEEKVNMYKVRPFG"
     gene            19705..22008
                     /locus_tag="PL000_01635"
     CDS             19705..22008
                     /locus_tag="PL000_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HNC87623.1"
                     /translation="MKNKMFLILGILVILLTIPLHAAAKAPEVDVQPEKYAAIEINGN
                     NAKVPFLDLGYRERTLNSPYGITNIYFNTPLSWKLIPGGEIEINYDVFLSGADLGKVV
                     DSANPYGGNLIFTFNGQIIGSTPLEGMGSHTVRLEIPDTALTSILQDGRHQLTISLEA
                     QFSCDYDVNARIVVNPTSFFDLFFEESSPELNLSRLPSPFYLENSFIPDSTLVVVADN
                     PDALELQSALNVMAGFGSMIGRVYDMQLITVSQLAGFDPTLYHMIFVGTADKFPVLTD
                     VNFKIPITDGKFVNLSPASETDGIVELALSPWNPTKVIMAVSGNSGDAVMKAGQAVSS
                     GRVFVYSDPTVAFVSNVQALSDTLPIVEDFTFESLGYVTETLSGIGLKSKEYIFYVSK
                     EQVLTKDAYVDLVYYHSGLLDYGVSSFSVELNGQVISSTPFSKESEQVTTLRIKFPPG
                     TLRFGENRLDIRASMIILPSCDTSGFSDPWFTVSNQSSIHLPVTLPGINAEPLLKDLK
                     FFPELFVTHSDLGDIAFIFPKSDVASWVIAGKLAYNLGQQFNPLIANLEAAFSDEVPQ
                     QVHDDNSMIILGLASETPFLSEFNDMLPAPFDMASNLASEKQMQVVYRIPEGVSVGYL
                     ELLLSPYNAEKSILVISGNDRNGVLLAGDSLLVNELESQLAGVFAVTNGKQVATGNAS
                     SPFSIVDNVVPGAEAVSTTPVPEILAGGPPLFERPVWLIPAIVLSSITVVGVLGYVAI
                     SAIAKRRQEKRKLLAGEEQTNQEEDKK"
     gene            complement(22088..22318)
                     /locus_tag="PL000_01640"
     CDS             complement(22088..22318)
                     /locus_tag="PL000_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87624.1"
                     /translation="MEDSQPNTQLTRMLLSRLERISADSYWAHRASGVRGALLRMLEK
                     TEAGRPVQRSEMKRLVDLGFIILENASLEKIK"
     gene            complement(22331..23212)
                     /locus_tag="PL000_01645"
     CDS             complement(22331..23212)
                     /locus_tag="PL000_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87625.1"
                     /translation="MNKSLMTRLFHRIQKKVRSFFVLDQWEILVAPETEYTSLSWEKL
                     KSLTPPKDRFWADPFVWVQNGIHYLFIEELLFSNHRGRIACLQLDKALNIVSNQVVLE
                     RPYHLSYPFLFEYSGQLYMIPETGKNNAIELYRCIQFPDQWQFEKTLIKNIRAVDTTL
                     LESDGKWWLFTNIHEEGGSSWDTLHLYYSDNPLSENWTAHPKNPIVKDIHSARPAGRI
                     FSHNRQLIRPSQNCSPRYGYAINFNEIITLNETEYAETTRHAFTPPSKGTIQATHTYN
                     SAAGITVIDANIRRRKF"
     gene            complement(23301..23373)
                     /locus_tag="PL000_01650"
     tRNA            complement(23301..23373)
                     /locus_tag="PL000_01650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23338..23340),aa:Arg,seq:tcg)
     gene            23552..24532
                     /locus_tag="PL000_01655"
     CDS             23552..24532
                     /locus_tag="PL000_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC87626.1"
                     /translation="MADIKAFAEQVIGNLEKVIIGKRQSVELIVIGLLCQGHVLIEDV
                     PGVGKTMMARGLARSLDCIFNRIQFTPDMLPSDVTGVSIYNQQKNKFEFRPGPIIGQI
                     ILADEINRATPKTQAALLEAMEEHQVTVDGVTHSLPEPFMVLATQNPIEYEGTFPLPE
                     AQLDRFLLRLRLGYPSMTDELRVLSDQQIQHPLETLKAVVKTKEILQAIDDVKHIYIS
                     QAVKNYIVELTTRTRQSTDVYLGASPRGSLALARAGRARAAMQGRDYVLPDDIQALAV
                     PVLGHRIIVSPAARLRDLSSDRIVQEVVQATPVPGGEFSPEITSTRKKTK"
     gene            24529..25845
                     /locus_tag="PL000_01660"
     CDS             24529..25845
                     /locus_tag="PL000_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC87627.1"
                     /translation="MRSGRILLALLLLIGIAGVLTTGAAIYSRFIYLSLLLGVGSWVW
                     TRWSASGMGLQRFSRLQRANVGDIFEEHFEVANNSRVIAPWIEVYNQSPIPFVSGSRL
                     FTFVLGRQKRTHLARTWLTRRGGFPLGPTRISTGDPFGLFRVSKEIPASQTLVVLPMI
                     FEIKSFLFPPGLLPGGQVIRRKSPDVTPHAAGVREYVHGDAMKRIHWPTSIRRNHLMV
                     KEFEQDPQAEVWLFLDLHRDVHLEKAYQFDEISVEAMMFGKRPRFQLPPSTLEYAITI
                     TASLTHYFIERHRAVGYASAGNTFTVHPADRSERQEGKVLETLAFVEANGKLSLAALV
                     SAQSAQMPQGSSAILVTPSTRPDLLLAVDDLQRRYLRPVVILLDGETFGGRRGSTAGL
                     VRALRERRVPVCVVACDANLSETLSEFSTEFISQDIRTWQTPVLSQ"
     gene            25818..26729
                     /locus_tag="PL000_01665"
     CDS             25818..26729
                     /locus_tag="PL000_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC87628.1"
                     /translation="MANTRIITIDLNFQNKTQAIASYLIRHGDANILIESGPGSTLSA
                     LKAGLAEEGLSPRNITHVLLTHIHLDHAGAAGWLSQQGAQVFVHPNGAAHMLNPEKLI
                     ASATRIYGDRMETLWGEFLPVEQNQLTVPKDAEEIAIGALRFLPIHTPGHAEHHYSYL
                     FEDLCFSGDVGGVRIPGFPYLRAPMPPPELHFAKWRESISHLKSLKFARIAPTHFGIF
                     DDVNWHLNELQKNLDEAEKWLEEIMPADPPVDELREKFTVWMNAQAASLNLSEDVLRA
                     YTLANPLGMGADGLMRYWKKVRSSPMA"
     gene            26832..27758
                     /gene="argF"
                     /locus_tag="PL000_01670"
     CDS             26832..27758
                     /gene="argF"
                     /locus_tag="PL000_01670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNC87629.1"
                     /translation="MKDFLAISDYSSEEIQDMLDLAAKLKKQYFKKGNKPIFKGKVLG
                     MIFQKPSLRTRVSFDMAMRHCGGDALYLSPSEIGLGKRESIADVARVLSGYVQGLMAR
                     VFDHEHVLELAKWSDIPVVNGLSDYNHPCQGMADALTIQEKFGKKSRGLNVSYVGDGN
                     NVTVSLMHVSALLGWNVTVATPEGYDVNPKAIEIAQKIAKKTKSKLTFVRDPHEAVKG
                     AHVIYTDTWTSMGQEEETAKREKIFPPYQVNAKLVGEASKDVIVMHCLPAHRNHELTD
                     DVADGKHSVIFPQAHNRLHAQKAILARLFDVA"
     gene            27848..29050
                     /gene="rocD"
                     /locus_tag="PL000_01675"
     CDS             27848..29050
                     /gene="rocD"
                     /locus_tag="PL000_01675"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HNC87630.1"
                     /translation="MNTQDFIKMEEQYGAHNYHPLDVVIEKAEGVWVYDVDGRKYLDC
                     LSAYSAVNQGHVHPEILKALLDQAKRVSLTSRAFRNDQLPLLYKELSEMTGYEMSLPM
                     NSGAEAVETAVKLARKWAYQVKKVPRHQAEIIVASGNFHGRTVTIVSFSTEPSYRDDF
                     GPFTPGFVTVTYGDADAIEKAITPNTAAVMLEPIQGEAGVIIPPAGYLKKVAEICKKN
                     NVLFVADEIQTGLARTGKLFASHHEDVRPDIVIIGKALAGGFYPVSAVLADKPILGLF
                     QPGEHGSTFGGNPLAAAVSRAALRVIREEKLAERSQQLGEYFVEQLSEISSPHIKEVR
                     GRGLLIGVELKPEAKGARRFCEALQTKGILAKETHDNVIRFAPPLVIDKETIDWALPS
                     IREVLNMK"
     gene            29105..30196
                     /gene="ald"
                     /locus_tag="PL000_01680"
     CDS             29105..30196
                     /gene="ald"
                     /locus_tag="PL000_01680"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNC87631.1"
                     /translation="MYIGIPKESRTFEYRVGLSPAGVEILTQNGHQAYVEHEAGVMAG
                     FSDADYEKAGARLVFSKEEVFGRADLLLKVSRPMQEELNWLRPESILAGFLHLASTQQ
                     DKIDILLEKKITSVAYEQIKAPDGSLPVLRPFSQIGGAMAAQIAARLLQNNWGGKGIL
                     MGGVPGVPPAEVVILGAGVVGTSAAQMFLGMGAHVTVIDQNLAALQRIWDRFPNIATK
                     MSTKRNIERSVMYADVVVGAVLSPGQRAPILVTREMVRSMKPRSVIMDVSIDEGGCVE
                     TSRPTTHEHPVYIEEGILHYCVPNMPGAVARTATHAFVNSAIPYITEIAALGTKEILS
                     NPSLEPAINTHDGKLLNLVRLSAQQQEDE"
     gene            30198..31508
                     /locus_tag="PL000_01685"
     CDS             30198..31508
                     /locus_tag="PL000_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNC87632.1"
                     /translation="MTTASIYQSRVTTAQEAVKTIKSGNRVFLTGNVSVPQKILAALV
                     EYAPNLTDVEICQALTVGSADYVGPEMEGHLRVNSMFISANIRKAVQEGRADFTPVLL
                     SEFPLLFKRGVLPLDVAVIHVSTPDEHGFCSLGVEVGLTKSAAESAKIIIAEVNEQMP
                     RTLGDAFIHVSRLTHIVPVNYPIPEHRMTAEGDMDIVQKIAGHVASLIPDGATMQLGI
                     GSIPDAVLKFLFDKKDLGIHTELFSDGVIDLVNAGVLTNARKSLHPGKVVAGFILGTK
                     RLYEWVNDNPMVEMHPTEYVNDPFIIAQNDRMVAVNSAIEVDLTGQICADSIGPKLYS
                     GVGGQLDFVYGASRSKGGVPIIALPSVATLRDGTQLSKITAMLKTGAGVVTSRNHVRF
                     VVTEHGIADLYGKTIRQRAQALINVAHPQFREELTRQAKELHYG"
     gene            31739..33226
                     /gene="pyk"
                     /locus_tag="PL000_01690"
     CDS             31739..33226
                     /gene="pyk"
                     /locus_tag="PL000_01690"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNC87633.1"
                     /translation="MRRAKIVATIGPASDSEQALEALMKAGMNIARLNFSHGTHEQHQ
                     KRVAAIRAVSKRLGMPIGILQDLQGPKIRVGKLAAPLQLAVDEHVCLYATNDEPPQTE
                     NQLLPVDFRELFDSVHEGDRLLLDDGRLALQVISAEGRVVHARVLVGGTLSSHKGINL
                     PGVKLRIAGFTEKDKTDLAFGISQGVDAVAISFVRSADDVRSVRAAIKEFSQGRFVTP
                     LLIAKLEKPEALNELEEILDVVDGVMVARGDLGVELPPERVPSLQKHIIRSANARAKL
                     VITATQMLESMIQNPLPTRAEASDVANAIFDGTDAVMLSAETASGEYPSEAVSMMSRI
                     VSEAESHFTEWGSRQDGRAGLGDSDAASMARAAKALATDPEVSAVSVFTMQGRSAWLM
                     SKGRPSKNILAFTPEYETFNQLSFLWGVQPNLTKFANSMDEMLANVDAVLLNSGIRSG
                     QQVVLVCGYPVGAQRPPNMALLHTVGSDASVNLARKLAESNVVKR"
     gene            complement(33342..34217)
                     /locus_tag="PL000_01695"
     CDS             complement(33342..34217)
                     /locus_tag="PL000_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC87634.1"
                     /translation="MHKHSVAGLFAGLGAASIWGGMYVVSKVVLEVIPPFALLSIRLV
                     MGALVLGIIIYFRKDKVVFSKEQFWKSFFVGVVGYGVSLGFQFVGTKLSTASNGSLVT
                     SATPAFVLIFAPFLLGEKTTTRRIVALVVSTLGVLAVIDPRNAELSPTLFWGNMSLLA
                     AALTWALYSVLVRKVSQSGDLLTSSTVMLLGGLPSSIAFGLWEVNTQGVGAITPGIIG
                     GLLFLGIVSTAIAMFLWNYAFAELPAAVASLTFFAQPVVGTLLGWFFLAEKITPLFLF
                     GGALISVGILIATRE"
     gene            complement(34270..34878)
                     /locus_tag="PL000_01700"
     CDS             complement(34270..34878)
                     /locus_tag="PL000_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HNC87635.1"
                     /translation="MDVFSLLDTVQTIARNGLLHVSNEYDKERYERLLEMAAKTYSDI
                     LNIPSEKIKQGFSNELGTITPKLGTNAAIFNERGEILLMERVDGSGWCLPAGFVEPNE
                     SPADGILREVREETGLEVEIKQLVGVFTRKPSAKMGPHTVVSVVHLCEIIGGELKISH
                     EGHALQYWAVDDMKNWHASHEIHARAAYKMWASERLIPAVSE"
     gene            complement(34901..>38641)
                     /locus_tag="PL000_01705"
     CDS             complement(34901..>38641)
                     /locus_tag="PL000_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC87636.1"
                     /translation="KISLANKQAENIFGYEAGGLQGIGIEQLVPEPIRHHHTFHRSDY
                     LNHPHPRQMEGRELTAIRRDGSTFPIQVGLNPIWNNGQISILVSAMDMSLQKQAEAQR
                     QSLMEIMQAAVETNNLQEYLAKVHAALKKALSAENYFVILKNKTTGMYEGAYSVDQIN
                     AALAPQSEMQKSASAYVIRTGKSLLLTEERFQQLIAEGEMELIGSRSLSWLGAPLKIS
                     GESIGVMVVQDYEKEFCYSKAEEEFFTSTAGQVALAVERKRAEEELRASQQHFYELFE
                     NVPVGIWESDFTSTREYLNELSDAGVTDFAAYFDAHPEAVNECVSRIKILNVNQAALQ
                     FYGVEDKSKLIDKSTTPIRIDSKSFRVFKEQFIALINGKVSYDTEFEALNMRGEIRTV
                     TMWLRLAYGAEQTWKRVFVVFSDITERKLAEQAIQTAEFRYRQIMEQIPAVVYSAGLG
                     PDMRWKFVSPQIKDLTGYTAEEWIADQQIWQRSLHPGDRQLVTDVETRSIITGEPVKL
                     EYRLYTRDGRMIWVRDLATVASDENGNPFLSLGFMFDITEQKLAEEQVRRRADEFALL
                     YDTAHSLAKSQADLRSVLQVIAARAESMLDSNSAGFYLYDATREQLDLAVRIGEMFPA
                     GPVRLKPGQGVSGLVVQTRKTIIVNEYSTWENRSPLFDGLNVKAVLGVPMVYGGEMIG
                     VLTIGELGSSHRKYTDADARLLELFASQAAAAIYNARLLENTIRRLDELETIARVSGT
                     LRAANSLEEMIPGLLDETLKALDTNAGSIYLYDPASGTLQSYIQRGWFGEKMPHSQRP
                     AEGIAGFVYSSGEVYHLKESMQEWKEIPNGWDAVCIPIQTSQEVVGVLFVSVQMPREL
                     SSEEVRLLTTLAEIAGNAIHRTRLHEQTKRNLTHISILRQIDQTIASNFDLSVSINML
                     INNALTQLQADAVSILRLRPATQMLEYFAGHGFKNEGMIRQVRLRLGDKHAGQAALER
                     RQVSITNLSNSDQFFSNSDFARLENIRAYHANPLIAKGEVKGVLEVFHREPFHPDKEW
                     LELMDTLAGQAALAIDNAELFNNLQRSNQDLKFAYDTTIEGWSRALDLRDRETEGHTQ
                     RVTEMTMLLASSMGLQADELVHVHRGALLHDIGKMGVPDQILLKPGRLTIKEWKIMRM
                     HPVYAYEMLQPIAYLRPALEIPYAHHERWDGSGYPRALKGEEIPLAARIFSVVDVWDA
                     LLSNRPYRRAWKQQRVVEYMETYSGKYFDPKVVNSFLTLMSK"
     assembly_gap    38642..38741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38742..39252)
                     /locus_tag="PL000_01710"
     CDS             complement(<38742..39252)
                     /locus_tag="PL000_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC87637.1"
                     /translation="MNKTSHFLKPPNKNTPLFHWVGMGIVFTFGVFYYLALAVFLPRY
                     GEGVTIFVVLPVAVAGWIFGKKIGVWVGVFSFPINLLLLALANRGQFDRAMSLRWAIE
                     IDFFLAVVAGFTSGYAGEIIQKKDKNPDWEGFLSAMEAVPHGMLIADSNGKISLANKQ
                     AENIFGYEAG"
     gene            complement(39477..40214)
                     /locus_tag="PL000_01715"
     CDS             complement(39477..40214)
                     /locus_tag="PL000_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87638.1"
                     /translation="MITALEEFSLNAWAALQTVHYDGWVLRFANGYTKRANSINPIHA
                     SSIPLDEKIRFCEEIYKSRQLPVVFKLTPHVYPEMLDEELSARGFQKSPSSTSVQTLK
                     LDFSNFHLPLQTELEESLSSAWLNAVCHMNGVAEENRETLRQILLNVVPRHCFLSLKF
                     DDRIVACGMGVLQSEFLGLFDIVTDPAFRNRGYGRQVVEGLLAWGQQNQAKQAYLQVE
                     LHNTVALHLYSSLGFKEQYQYWYRSKP"
     gene            complement(40220..40618)
                     /locus_tag="PL000_01720"
     CDS             complement(40220..40618)
                     /locus_tag="PL000_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC87639.1"
                     /translation="MADMAKDFIKAAKKADLDALKALLKADKSLLNARDTDGSTALHC
                     ATWKGHREVVAFLLKVGADVHAQNSNDHWGTTPLHAAAHANQAVLVQMLIEAGADVNA
                     KDMNEKTPLHHTTFHKATAAAKVLVQHGGK"
     gene            complement(40646..41443)
                     /locus_tag="PL000_01725"
     CDS             complement(40646..41443)
                     /locus_tag="PL000_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC87640.1"
                     /translation="MDFSFIQITDHHLTDSDSEFLRGFSTRYALRTVLRHIAHNAGTH
                     VDFIVSTGDLVDIPSEASYQASLELLGVQNNLAEIPGPLHISVEGLQNLPMFVLPGNH
                     DDRKNFYKYLFPQSQSASIMNAAFIHKGIQFICLDWGPESKATAHPEMLEFLAQTIKK
                     DIPSIIMMHHQLVKLGSKWLDDFIADDTSRFWEIIAQKPVLGIFCGHVHATYESIVNE
                     VPIFGLRSTAPQFVLQDEPLACLLPPHYRLVTIQNDTLTTQIFEVPL"
     gene            complement(41481..41876)
                     /locus_tag="PL000_01730"
     CDS             complement(41481..41876)
                     /locus_tag="PL000_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC87641.1"
                     /translation="MSPSIAHVTYLVRDYDEAIAFFTEKLNFILLEDTPLDDNKRWVR
                     VSPQGGNGSALLLAKADTAEQTQHIGNQTGGRVAFFLHTDDFQRDHQHMQSHGVRFAE
                     DPRHEPYGIVAVFFDLYGNKWDLLELRNL"
     gene            complement(41883..42281)
                     /gene="arfB"
                     /locus_tag="PL000_01735"
     CDS             complement(41883..42281)
                     /gene="arfB"
                     /locus_tag="PL000_01735"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNC87642.1"
                     /translation="MKNLESEISLEYIRASGPGGQNVNKVATAVQLRFDITNSPSLAS
                     DVKGRLIRLSGSRVTAEGVLIIEAKKYRTQEQNREDALQRFYDLVAKANEKPKVRHKT
                     KPTKASKEERLKEKKNRGSIKKIRRDKAYD"
     gene            complement(42334..43659)
                     /locus_tag="PL000_01740"
     CDS             complement(42334..43659)
                     /locus_tag="PL000_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC87643.1"
                     /translation="MTTETTNEKLDFKRILPVLVIVLVDLMGLSIIIPLLPLYAARFG
                     SSPLVIGILQATYPMMQFLGAPILGRLSDRFGRKPILVVSQIGTLIGFIMLGFANSLL
                     LLFISRIIDGLSGANISTAQAAIADSTTEKTRTQGLGLVGAAFGIGFVLGPILAFIVL
                     AATGQNYQAVAFTAAFFSLASILLTLFWFKETYAEVENPEASRKRRPFSFGALREALS
                     RPSIGFLLILMFFYQIAFGGYEQLFSLFTLTRLGMDARDTSGLFVLAGLFIIVVQGGL
                     IGRWSKQKGDRWLVLMGIGTLAIGLIGTALTPAVPIPWYEKAKVLESMAGQGEFRVSI
                     QSINIQLPDEAAKGWLGIIWLLLASFPAALGGGVLQPAVNSLITKASDKNEVGGMLGA
                     SSAAYSAANAIAPLFYGALFQWFGGPVPFFAGGLILAIMWLLAPRVVKA"
     gene            complement(43737..44795)
                     /locus_tag="PL000_01745"
     CDS             complement(43737..44795)
                     /locus_tag="PL000_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNC87644.1"
                     /translation="MTEALPMAGTPATNVNLAGLSKNEYRGNPSTLCQGCGHNSISNQ
                     IVTALYEMNIVPEDVVKFSGIGCSSKSPTYFLNRSFGFNSLHGRMPSIATGALFGDHH
                     LKGIGVSGDGDSASIGMGQFKHVMRRNVNMLYIVENNGVYGLTKGQFSATAEKGLSLK
                     KQGENPYMPVDICMEALVSNATFIARSFAGNPKQVKELLKAALAHDGIAVLDIISPCV
                     TFNNQENAYHSYAWGKDHEEPLHEISYIAPRNEIILEREMEDGEVREVAMHDGSTVIL
                     KNLEKGYNPTNRFEALRVMEEAGRNNWLITGLLYVDTSKPNLTSMYNLVDTPLNRLTE
                     AELRPDRSMIDKVNALMF"
     gene            complement(44820..46757)
                     /locus_tag="PL000_01750"
     CDS             complement(44820..46757)
                     /locus_tag="PL000_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNC87645.1"
                     /translation="MNKIINDFSITVGTVNGSGSSTANNTILRSIFKMGIPVSGKNLF
                     PSNIQGLPTWYTIRANKDGFIAHNERRDIVIAMNPNSFVRDLASVTPGGAFFYADDIK
                     QPINRTDIAIYPMPVKTIVKNDPNVPTDFRDLVGNMVYVGVLAQMINLDMDKILAALT
                     FHFKGKQKPIDMNYGAVKAGAEWARSNLEKKDPFYVETMNKTEGMIMADGNTAAAIGS
                     IFGGVQFAAWYPITPATSLAESLNDYLPQLRKREDGKHTYAVVQAEDELAALGMTVGA
                     GWAGLRSMTSTSGPGLSLMTEFAGLAYYAETPVVIWDVQRIGPSTGLPTRTSQGDLTF
                     THFIGHGDSHSVTLLPGDVFECFEFGWRAFDVAERIQTPVFVLSDLDMGMNQWMSKPF
                     EYPSTPMDRGKVLFEKDIEELKGNWGRYLDKDGDGIGFRTFPGNKHPLSSYFTRGTGH
                     DEYAKYSEEADIYLKNMDRLKRKHETAKKYVPAPVMHSIKGATVGIIAYGSTEAAVVE
                     AQHQLEKDHGIKTDFMRVRAIPFTKEVDAFLAKYDQIFIVEMNRDGQMYQILLTEYPQ
                     FANRFKSVAFGDGMPASAKWVREGILAKYAKAGTPKKAAAAKTASKAKAVSGSKKAAT
                     KKPAAKKPVAKSKSSVKSKRK"
     gene            complement(46771..47448)
                     /locus_tag="PL000_01755"
     CDS             complement(46771..47448)
                     /locus_tag="PL000_01755"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HNC87646.1"
                     /translation="MVLRDRISPELSEFLRYLASHEEATGGLPSLNDLSRELGISLAA
                     LREQLEVARALGLVEVRPRTGTRRLPYSFAPSVMKSLAYALTLDQSNFEKYSELRNHI
                     ETAYWDEAVSLLTEEDKLELKNITARAFEKLHAKQVQVPHEEHRKLHMLIYSRLNNPF
                     VTGLLEAYWNAYEAVGLNMYAGGIDYLEEVWGYHKTMVQSICNGNYAAGRKALITHID
                     LLAHRPT"
     gene            complement(47607..48092)
                     /locus_tag="PL000_01760"
     CDS             complement(47607..48092)
                     /locus_tag="PL000_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87647.1"
                     /translation="MNKALIFSLTLFTILIAACGTQATQTPPSPATVTEAPTIPVATD
                     TAIPAPTEAPILQAETPAQTQETAATVSFAKDIMPIFEASCTKCHGVEQVKEGLDLRT
                     YETLMAGSFNGSVITPGNANDSFLVEQIVEGEMPKRGQKLLPAQIQLIIDWINAGALN
                     N"
     gene            48274..49845
                     /locus_tag="PL000_01765"
     CDS             48274..49845
                     /locus_tag="PL000_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87648.1"
                     /translation="MAGAFSLPNEYWLSIQITPQDVENLHNYLFERETPLTTRDLSVE
                     LVETRIKAERAVAEGKKKAAGRSYLPKEKYQVGEELIFPALDWKRGTVTALREGVNPS
                     VTGFDVLTVTMEDRVERMFAANLEAHYLNDAPIVEPETNEMSAEMVLREFGEQIEKKL
                     EASFTADSGLVKIAGSWFPRALLIDINQGQLNLAEAVLDMAGGEPLTTEALMKDIELP
                     ASVNPKLAEFSLNYALQSDERFDEVGPAGQVLWCLYRLEPDLVREVPWPLRYSEIEHD
                     RSVLGDEIKRLEDQLDDELTPVDEDDARGDISSITITLIYPHLRAGTLPMSARARRLF
                     PTAYESSRVRFTLVDGKTKQSIPAWVVLKHGYVFGLREWYKAHQLIPGSIVQLKRGEK
                     PGEVIVEVKSQRSSKDWVRTVLVGKDGGFVFAMLKQPITAEFNDRMVIHVPDFKSLDP
                     VWEKKQSFNELVLSVMRELTKSNPQGHVHAQELYAAINLVRRVPPAPLFALLESNPAF
                     KHVGDLHFRLDEEAE"
     gene            49842..50285
                     /locus_tag="PL000_01770"
     CDS             49842..50285
                     /locus_tag="PL000_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87649.1"
                     /translation="MSLLKPNAKTPFHIDFEWWKKNERDWHVFLRSLLCPEHQEAFAE
                     MDEGGMIDWIDPQTAEVRQVAGVQHALMSHCALLPEFSDARTAMVEAVFRTFLVNGNT
                     PMSAEELSQKLGRPADTILKTIAGPRVYRGLRPFEVGLPIAESGE"
     gene            50295..50370
                     /locus_tag="PL000_01775"
     tRNA            50295..50370
                     /locus_tag="PL000_01775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50328..50330,aa:Arg,seq:tct)
     gene            50410..50811
                     /locus_tag="PL000_01780"
     CDS             50410..50811
                     /locus_tag="PL000_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC87650.1"
                     /translation="MPAIVRKSSAALLETRREILHAVSWHVRSNVWSPPTDQYETEDT
                     YVVRVEIAGMNEDDFEVSVENNNTLLIAGVRTDAQERRAYHQMEIRFGKFATALGLPG
                     PVDIEHALAEYKDGFLTVTLPKATPKQIKVE"
     gene            50864..53443
                     /gene="lon"
                     /locus_tag="PL000_01785"
     CDS             50864..53443
                     /gene="lon"
                     /locus_tag="PL000_01785"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC87651.1"
                     /translation="MGETDEEMIKMFMTSALARFQKDDGASHAPQPDDKNEGQKFPDT
                     LPILPLRGVVVYPNTAVPLTVGQPRSIKLVDDVVGGDKLVGLVAALNPELETPGPNEL
                     YQIGTIATVHRLLRAPDGTIRLLVQGMERFRVEEFVADEPYLKARISLIPETVESNLE
                     TNALARNARDQFQQITQMIPSFPDELVGSITSLEDPLQTVYTIANFQRINLQDAQEIL
                     ELDSVYEKLKKLIGLLVREAEVLQIGQKIQNEARGEIEKVQREYFLREQMKAIQRELG
                     DRDEQSVEADEFRKKIEEANMPEEAHKQAKQELDRLSRLPTAAAEYGVIRTYLDWLVS
                     LPWSKSTQDNLEIAHAREILNKDHFGLEDVKDRILEFLAIRKLRIDRKDDEKAPSDDL
                     IRREREGVILCFVGPPGVGKTSLGQSIARAMERKFIRASLGGVRDEAEIRGHRRTYIG
                     AMPGRILQSLRRVDSKNPVFMLDEVDKLTYDFHGDPASALLEVLDPEQNSEFRDNYLE
                     VSFDLSQVFFITTANTLETIPGPLRDRMEIIFLSGYTENEKMSIARGYLIPRQIRENS
                     LRAEEISFTDDALRMIVRQYTREAGVRNLERKIGAVCRKVGTKIAEHKGEKFEITPEL
                     VEEYLDHPIFLGTEELNKRTSIPGVVPGLAWTSFGGDILFIETTAMPGGRGFQVTGSI
                     GNVMQESARAALSYVRSRAASLNVPEEFFNKYDLHMHIPAGAQPKDGPSAGVAMATSL
                     VSLISGRKVKPQIGMTGEITLRGQVLAIGGVKEKVLAAHRNGLTTVILPKRNETDIDD
                     VPDEIRQSMKFIFAESVEDVINAALEKNKPEEKKAGKPRRKKSQPTKVKKNGKSKSTN
                     RRR"
     gene            53409..53774
                     /locus_tag="PL000_01790"
     CDS             53409..53774
                     /locus_tag="PL000_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87652.1"
                     /translation="MAKVKVLIAEDDITMVSLLKTLLKMEGFEVLALQPDADVPLAVR
                     NEKPDIVLLDVHLFGQSGLDILSDIREATETATTRVVMSSGSDVREECARRGANGFLM
                     KPYMPDDLITILKQVLQAA"
     gene            53796..54290
                     /locus_tag="PL000_01795"
     CDS             53796..54290
                     /locus_tag="PL000_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HNC87653.1"
                     /translation="MAIRIGIMTLSDRSARGEREDASGPALADLVHAQGWSVVEQAIF
                     PDDEPAIRTALVTWADSGRFDIILTTGGTGFAPRDVTPEATRAIIQREAPGLAEAMRA
                     DSLKKTAHAMLSRAVAGIRGRTLIVNLPGSPKGAVENLQAILPVLPHAVQLLSNDPAS
                     EASH"
     gene            54403..54960
                     /gene="efp"
                     /locus_tag="PL000_01800"
     CDS             54403..54960
                     /gene="efp"
                     /locus_tag="PL000_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC87654.1"
                     /translation="MIDVNELRKGVTFELDGNLLKVLDYSHNKTGRGNANIKVKARNL
                     LTGANIERTFNSGQSVQDVSLDFANVSYLYNDGDLYYFMDNATFEQPGIKKESLGDSA
                     KFLTEGMEVKLTFYKGEAIDVELPTSVDLKVVEAEMAIRGDTATGVTKKVITETGVVV
                     QCPNFVVVGDVIRVDTRTGDYVTRV"
     gene            55077..55406
                     /locus_tag="PL000_01805"
     CDS             55077..55406
                     /locus_tag="PL000_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87655.1"
                     /translation="MKTPAGRECPHFYGNYFRGRNDEECRLLKLHGQAWTRDLCKTCP
                     VPEIARANSCQHMQLKVTVERPILAMFQRRVQVSAFCEKSNQNVSEPQIGCGKCHDLP
                     FKFEIKE"
     gene            55437..56630
                     /locus_tag="PL000_01810"
     CDS             55437..56630
                     /locus_tag="PL000_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC87656.1"
                     /translation="MTKPILLLDLDDTLLNTNMEAFVPAYFQALAKELSTYVTPTAML
                     PALMAGTNAMNVSDDFSRTLEQVFNAEFYPRMDASPETLRPPLENFYDHVFPLLGNVT
                     SQRPEALEFVQWALSKGYRIAIATDPLFPLKATHSRLRWAGFEPEQFEIVSSFEHFHF
                     SKTHPAYYAEILGRMGWPEVPVLMVGNDIERDIKPSKMLGLAAFFVENEPASQSGPEA
                     GLRGNLVDLRRWLESTDLSALIPSFKSKDAVLGILSATPAAMNSLLSGLDETTWPREH
                     SDEDWAMIEIICHMRDTEREIHQMQIRLFAEQSEPFIPRPDTGVWASQRDYLHENGPQ
                     ALKEFNDARRETLALLSEVSAEAWGRRARHAIFGPTDFLEVVSFMADHDRMHLQQIWT
                     TLKKL"
     gene            56752..57993
                     /gene="fabF"
                     /locus_tag="PL000_01815"
     CDS             56752..57993
                     /gene="fabF"
                     /locus_tag="PL000_01815"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC87657.1"
                     /translation="MRKRVVVTGLGCVSPVGNTTKDTWQALLEGKSGAAPITTFDASA
                     HKTKFAAEVKGFDAAALFGTRDARKMDRFTQFATAATLEAMAQSGLKVDESNRDRIGI
                     LIGTGIGGISSLLEQYDIMRERGPERVSPFLIPMMISDGAAGNIAIRLGVRGPNMSLA
                     TACASGTNALGEAAEMIRRGAADVMIAGASEAAINAIAMAGMNSMTALSTRNDEPQRA
                     SRPFDKDRDGFMMGEGAGVLILESLEFAQARGANILCEFSGYGTTDDAHHISAPAEDG
                     AGAVGSMSLAMEDAGLKLEEIDYINAHGTSTYLNDKSETSAIKTVFGERAYKIPVSST
                     KSMTGHLLGASGAVEAVFCAMAIMENILPPTMNYETPDPVCDLDYVPNQPRKAEPNHV
                     MSNSFGFGGHNATLILSRFNK"
     gene            58004..59107
                     /locus_tag="PL000_01820"
     CDS             58004..59107
                     /locus_tag="PL000_01820"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNC87658.1"
                     /translation="MPFAHVTGWGMYVPEPVLTNDDISKMVDTNDQWIRERTGIRERR
                     IAREHEFPSTMGVEAAVKALQVANLAPTELDLIICTSSSPEYIFPATACIIQDQIGAT
                     KAGAFDLQAACTGFIFAVNMAAQAIRSGSIKNALVVGTEVLSRFVNWKDRGTCILFGD
                     GAGAFVLQASDVPGGVLSAVMHSDGSGADTLTLPGGGSHFPASELTIHEGKHFVHMDG
                     KEVFRFATRVMGRAVMESLEQLKMTTDDIHCIIPHQANYRIIETAAKYLKMPLDKFVI
                     NVDRYGNTSTASIPIATVEAIQNGRLKSGDKAVFVGFGGGLTWGAMVIQWTGPIPAKK
                     NVHPEQYRYFARLRSLLRRVWRYIEGFFSRKSL"
     gene            59165..59353
                     /gene="tatA"
                     /locus_tag="PL000_01825"
     CDS             59165..59353
                     /gene="tatA"
                     /locus_tag="PL000_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC87659.1"
                     /translation="MLGLPRGAEWIIILIIIVLLFGPGRIGKVAGELGRSIKSFREGL
                     NPGEEQAADTAEKKEENK"
     gene            complement(59427..60065)
                     /locus_tag="PL000_01830"
     CDS             complement(59427..60065)
                     /locus_tag="PL000_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNC87660.1"
                     /translation="MEMFYENLIKILLAVVVGGVIGAEREYQDKAAGFRTIILITLGS
                     ALFTIFSVTMDPGFTRSRVAANIVTGIGFLGAGAIVREGGKIGGLTTAATIWLSAALG
                     MGIGAGDVYISLTATVITLLVLLVFPRIEARIDRVREARSYRIVIGASGFQKIDKVEK
                     TLKECSLRVLEHHQSKSESEIISVWKTIGSPSNQEKFVQLMLKDKDIRELDY"
     gene            complement(60116..62161)
                     /locus_tag="PL000_01835"
     CDS             complement(60116..62161)
                     /locus_tag="PL000_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC87661.1"
                     /translation="MNRLSQATSPYLLQHAHNPVDWYPWGSEAIEKAKAEDKPIFLSI
                     GYAACHWCHVLAHESFEDPETAAFMNEHFINIKVDREERPDLDGIYMQAVVAMTGSGG
                     WPMSVFLSHDLKPFYAGTYFPPVRRFNLPAFMDVLTGLANAWKNDRAEIEKVGEQVNG
                     HLQYQNQSKNTEAITQEHLEPIAKALQGAYYWGYGGWGDAPKFPQPMALEFLLHHSIA
                     NKKDEYLKLIDHCLRIMARGGMYDVVGGGFSRYSTDNFWRVPHFEKMLYDNALLARAY
                     LHAWQVTKEPFFKQIVEETLGFVARELTHEQGGFYSSLDADSEGEEGRFYVWTLDEIR
                     AVLKDDSEFFEAAYGISPQGNWEGKTVLQRALDDTTLAARFKLDPETVPAKLAESHSR
                     LLSARASRIRPGTDDKVLTAWNGLMLAAFAESARVLAGSSPHSHTHLDLATRSAEFLV
                     NNLRVNGKLHRSWRNGQVTEEVFLEDYAALILGLLELYQTDFNNKWFTIAHELTDEMI
                     EKFSNPDGGFFDTPHTGETLLFRPKDISDNATPSGNALACEALLKMAAYTDQGSYRSL
                     AEQSLALIGESILRYPLGFARWLSAAENALGTMKQIAVLGEAGEENFERLLKVIRAEY
                     RPGVVVAASSHPPSQHAPALLENRPLLNGKATVYVCEGFVCKQPTNEIEVLIAQLKA"
     gene            62245..62697
                     /locus_tag="PL000_01840"
     CDS             62245..62697
                     /locus_tag="PL000_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87662.1"
                     /translation="MKKYSILLAIVALVLASLACQTIMGGGDNGFEAPDVPQQTDGDD
                     VEVPSVEQDGGDITVGGESPFPVTSDAFNVISTPQSVTYQTNMSGDDVMKFYRDELGK
                     QGYTEDTSMTTNFSGMIAMFFVNNGKTIVIGGAPVGDGSTSVTVAYQQ"
     gene            complement(62771..64477)
                     /gene="pabB"
                     /locus_tag="PL000_01845"
     CDS             complement(62771..64477)
                     /gene="pabB"
                     /locus_tag="PL000_01845"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNC87663.1"
                     /translation="MIKKDEIILRNDREWLHFSKPHRVISTENIHNAGETLLEIERLV
                     NENHWYAAGFVSYEAAPAFDSALRVIESDEFPLLWFGLYDPPQSVQLPSTVKDFSNLH
                     WEPTTERSVYNAAITKVKEQIASGKTYQVNYTMRLQTEFTEDPWEFFLFMAQTQNKYA
                     AYIDTGRHVICSASPELFFELDGEMLTGRPMKGTVKRGRTTKEDGELANWLHHSVKNR
                     AENVMIVDMIRNDFGHIAEIGSVHVPQLFTVETYPTLLQMTSTVKAKTKAPLAEIFNA
                     LFPCASITGAPKVSTMNIIAELETTPRKIYTGSIGTIAPNRKARFNVAIRTALIDRKN
                     NKAEYGVGGGIVWDSTSTDEYSEALLKARVLNQRPQGFSLLETLLWQPESGYFLFEKH
                     LERMADSAAYFGFEFSPETLHTHLATLAENFTSPKRVRVLADRFGGLTSEAKDFHPQE
                     KPFKITLAQKPIDSQNVFLFHKTTKRDMYPAPAYDDILLFNEKEELTEFTIGNLVIET
                     DEGKFTPPISCGLLGGTFRAHLLETGQISERVIQKDELANCKAIFLVNSVRGWVKVEP
                     GF"
     gene            64490..64858
                     /locus_tag="PL000_01850"
     CDS             64490..64858
                     /locus_tag="PL000_01850"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNC87664.1"
                     /translation="MKKLLPIVPRWLPALLMMLAIFAFSSRTSKELPNYGGWDYFIKK
                     GAHAVGYGLLALAYLDALPRRNYALAWLLALLYSATDEFHQSFVPGRHAAWTDVLVFD
                     NLGAMIALFVHYRKSRNVDL"
     gene            64893..66308
                     /locus_tag="PL000_01855"
     CDS             64893..66308
                     /locus_tag="PL000_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC87665.1"
                     /translation="MKNRITRRDFLKALSIGTASALALSCGAVTPQPEEEETSEPMPN
                     QDTSTSTSADVLVLGAGIAGLAAARTLADRGMSVIVLEARNRVGGRMWTDTSLGLPLD
                     LGASWIHGVNGNPIAKLAKQFGVATAPTDDENGIAFASDGSELPDDEFARMEALFEDI
                     YEEVAAMQEDTDEDMPLQEAFDEVIASRDLSEADLRRLNYYAYLGTALEYGADMKDLS
                     LWEWDQDEEFDGEEVIFPGGYNQITDGLAKGLDIRLGMVVNTVRYGADGVEVETSGGS
                     FVADKAVVTFPLGVLKQASVKFDPPLPESKQTAIGRLAMGVLNKVYLKFPEAFWDESI
                     ETINYMGETLGEWCDWLSFAPFTGEAVLMAFHGGDKGFDLEDLSDDEIREGAMKTLRV
                     MFGDDIPEPEGMLVTRWGKDPFAFGAYSHIPPFASGDDYDALFEPVEDVLFFAGEATW
                     REHPSTVHGAYLSGVAAAGEI"
     gene            complement(66377..66958)
                     /locus_tag="PL000_01860"
     CDS             complement(66377..66958)
                     /locus_tag="PL000_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87666.1"
                     /translation="MKKIFYLLPVFFTLLACGAQPSPTQDISNIVNATLTAVAQNSPQ
                     METPQTAPTQDISSAVNATLTAVAQNNPQGSEQQPVVAQVTGSLRIEQEQGSGAICTA
                     GTTYFVYAEFSSNGPATAQYQIYITDASGQVPNGTFEGYNAPQAEGTLTFDSAKTETL
                     TWRITGPYQYPEALTIRGNLNGQSLQSVPVACQ"
     gene            complement(67063..67836)
                     /locus_tag="PL000_01865"
     CDS             complement(67063..67836)
                     /locus_tag="PL000_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNC87667.1"
                     /translation="MENLLGHQISGYKVQTPVYEGPLDLLLSLIERAELNITAISLAS
                     VTDQYLSYLHGLEQMKPDEISSFLVIAAKLVQIKSEALLPRPIEREPGEEDPGVELVE
                     LLISYKRYKEIAGWLAERQENNLRTHLRVAPPPKVEAKLDLSNLTLEGLLAAAEAGIA
                     RGKEKKPLATVISTPKVTIREKIDYITKTLKNIQRMSFSGLITDKATRVEIVVTFLAL
                     LELVKRYRVTAKQDVLFGDIEFERSEDWKDDEEIDIEFE"
     gene            67871..68347
                     /gene="moaC"
                     /locus_tag="PL000_01870"
     CDS             67871..68347
                     /gene="moaC"
                     /locus_tag="PL000_01870"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNC87668.1"
                     /translation="MSKLTHLDEQGRAHMVDVGAKPDTERTAIARGEIHMKKETFELI
                     RDGQVKKGDVLTVAQIAGISASKRTSELIPLCHPLFLSKVDVTLVLEESLPGVVITAT
                     AKTTGKTGVEMEALTAVSVAALTVYDMAKAAEKTMRIQNIRLVEKHGGQSGDVVNE"
     gene            68340..68714
                     /locus_tag="PL000_01875"
     CDS             68340..68714
                     /locus_tag="PL000_01875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC87669.1"
                     /translation="MNNMEYSPVNFQEKFSRFSEQWSPKIIAQLNDYHLKLAKVQGEF
                     VWHDHPETDEVFIVIKGHLDILFRDGKVSLTEGEMFVVPKGVEHKPVAENECHILLIE
                     PAGTVNTGDVTDKLTAPNNVWI"
     gene            68716..68964
                     /locus_tag="PL000_01880"
     CDS             68716..68964
                     /locus_tag="PL000_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC87670.1"
                     /translation="MNHIKLLFFATIRDRAGVKSLEMDIPADLTIQGLKDKLSVEYPS
                     LKESMQSVLITINREYAFDEAIVPPNAEIGMFPPVSGG"
     gene            68973..69440
                     /locus_tag="PL000_01885"
     CDS             68973..69440
                     /locus_tag="PL000_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNC87671.1"
                     /translation="MNSSNLPTIFSITESEIDLNDLLARITLTSTGAAAIFTGMVRGV
                     TTRGDAHETEYLEYESYVPMAEAKMKQVADEIRAKWPVIEGIAVVQRIGKLYPMTPTV
                     LIACTAAHRDTGVFDAARYGIDRLKEIVPVWKKEVGPNGEEWVEGDYIPKPGE"
     gene            69442..69996
                     /locus_tag="PL000_01890"
     CDS             69442..69996
                     /locus_tag="PL000_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87672.1"
                     /translation="MILNSKRLIIRNFKDSDIESFLLYRNIPEVAKYQGWKLPYPREA
                     AVQLVDEMRNIDSPQAGRWLQLMLELRDTHEVIGDIGIRLKREDPRQAVIGFTLAPAH
                     WRKGYMTEAVVTILGFLFDVLNLHRVSADCDTENIASWRTLEKAGFRREAHFVENYPM
                     GDFYASEYIYSILQREWQAGKTPG"
     gene            70134..71372
                     /gene="fabF"
                     /locus_tag="PL000_01895"
     CDS             70134..71372
                     /gene="fabF"
                     /locus_tag="PL000_01895"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC87673.1"
                     /translation="MTERIVITGMGTVNPLGLNVKETWSNMVAGVSGVAPITLFDSGP
                     LQVHIAAEVKGFKPEEYMDAKEARRRDRFEQFAITAAKEALFQSGLQVTEKNAGRIGV
                     IISSAIGGLHSLQEATLTNYKEGPKRVSPFLIPMLMPNGGAGMAAIEFGIKGPCFSVA
                     SACASGADGIGTALMMLHTGMIDAALAGAAEATITSTGVAAFDRVGAMSRRNDDFSMT
                     PQPFDKNRDGLVMGEGAGVLVLEREADAKARGANILAELAGYGATADAYHVTAPHEHG
                     EGGTAAIMMALSSAKANIQDVGYINAHGTGTLLNDQAETRAIKAAFGELAYKVPISST
                     KSMTGHMMGATGALEAIICVQAVRDGILPPTIHYETPDPECDLDYIPNKAREADISLA
                     ISNAFGFGGHNAVLAVRKYA"
     gene            71460..71807
                     /gene="rplS"
                     /locus_tag="PL000_01900"
     CDS             71460..71807
                     /gene="rplS"
                     /locus_tag="PL000_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNC87674.1"
                     /translation="MTDFIKAVEAKPNEKIPQMSSGDTVSVHVKIKEGDRERIQEFKG
                     IVIRLRKGGNDASVTVRRMATNGIGVERTFLLRSPRIDKVVVERHSKVRRAQLYFMRG
                     RTGKSARLKQKFD"
     gene            71890..72603
                     /locus_tag="PL000_01905"
     CDS             71890..72603
                     /locus_tag="PL000_01905"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNC87675.1"
                     /translation="MPKPLIGITTRNGKDADGHPLTALQHSYINAIVQAGGMPILIPS
                     MLSEEDFTGLYSLLAGILFTGGGDVSLEYFNGSAHPRIGEVDKRRDTTEITLMRAAVN
                     DGKPLLGICRGAQVMNVALGGTLYTHIHDQVKGALDHDYPGDLRKVIVHPVNVDESTR
                     SAEIFGETLLHVNSLHHQGLKDIAPGLRVAGHAPDGLVEVVEIPDHPYAVAVQWHPEW
                     LTDQPAMQKLFKSFVDAAG"
     gene            72626..73474
                     /gene="murI"
                     /locus_tag="PL000_01910"
     CDS             72626..73474
                     /gene="murI"
                     /locus_tag="PL000_01910"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNC87676.1"
                     /translation="MMNLQNRQDAITSPIGVFDSGVGGLSVLRSIREQMPEESVLYFG
                     DQGHVPYGPRSMGQIQSFSAGITRFLLDHGSKLIVVACNTASAAALTYLRESFPGVSF
                     VGMEPAVKPAAETTKTGRVGVLATPATFQGALYASVVERFGAGVELFQHTCPGLVSQI
                     EKGELDSAATRAILEDALHPMLEKNIDTVVLGCTHYPFVIPLIQEVVGGAERVRVIDP
                     APSVAKQAKRLLEAAGMKNPAGSRASIRFFTSGDVESMKSMLPVLLGEMGDVELAVWQ
                     NDEEVK"
     gene            complement(73508..73951)
                     /locus_tag="PL000_01915"
     CDS             complement(73508..73951)
                     /locus_tag="PL000_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNC87677.1"
                     /translation="MPGENMRPDWDSYFMKIAYAVSERSTCDRAFVGCVLVLEKRILT
                     TGFNGSPAGQEHCDEVGHLMVEGHCVRTIHAETNAIIQAALHGVSTKGATCYVTHLPC
                     IQCTKALINAGITRIVYSTAYRTDENALNFLKAANIEVEQKEFRP"
     gene            complement(74028..75875)
                     /gene="glmS"
                     /locus_tag="PL000_01920"
     CDS             complement(74028..75875)
                     /gene="glmS"
                     /locus_tag="PL000_01920"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC87678.1"
                     /translation="MCGIVGYVGPRDAVSIILNGLKRLEYRGYDSAGVAVINSNQIEV
                     RRDAGKLSQLIDLVVKSPLTGAPGIGHTRWATHGAPSARNAHPHVGNSGKVVVVHNGI
                     VENFLELKDELVSEGVNFLSDTDTETIVHLAEHYQAANPGAGFVEAARRTFQKIEGAN
                     VVVLMSADEPDKIVTARIGNAGGVVIGLGENENFIASDIPAILEHTRKVIFLESRQMA
                     IVTREGVRIETLEGVEVKPEIHTIAWDAVAAEKGEYRHFMQKEIHEQVRALTDTLAGR
                     VDFKEGRIRLPELNLTPELAKRIQRIYITACGTAAYAGMVGKYLIEKIARVPVEVVIG
                     SEFRYSDPIVDENTVILAISQSGETADTLAAMEEGRRKGGIIWSIVNAVGSQAMRVSN
                     GTVAMQTGPEIGVASTKAFTAPLVDQYMLAILLADMRGVIDEKTRKALVADLRLVPDL
                     AGRVLDTEPEVEKVAHALKDIKGCLYLGRGINMPIAYEGALKLKEISYIHAEGYPAGE
                     MKHGPIALIDEEMPVLCIAPKDPWHEKMISQIQQAKARGGMVIAVATEGDELVPGMAD
                     HVLWIPEAPWMLSPIITVLPLQLLAYHIATIRGLDVDQPRNLAKSVTVE"
     gene            complement(76409..78145)
                     /locus_tag="PL000_01925"
     CDS             complement(76409..78145)
                     /locus_tag="PL000_01925"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNC87679.1"
                     /translation="MNIRLLKKSKPATGRKRAQGMLEFALVLPILLLVLIGTIEFGRL
                     FYAWLIIENSTRFGVRYAVTGQFEEIYCTGGEMDGDGSLCDGTSELDEIELARIPSIE
                     DETRRIIIGLHYGISQEDYDTRDDESGGNPPAPDYEILTQADNDYLNITVCSTAGSAR
                     IWQPPLMGQPVYASCTLGGVSDEFAGVPGQYVVVAADYNFAFMVLPVFKIEPSMIHLA
                     SFRQGVVEQFYATRAINTPIILAGPPTNTPLPTDTPTNTATFTITPSPTITPSPTLTS
                     TSTPTQTFTPSRTPTKTLTPTFTKTPTKTYTPTITKTPTSTPTPSCASIFIDRTQFNG
                     DSFEARVRNNNVATAYLTETELTWDPSPLTGGRYFDFTKWNGTTYYNPANTANTNSPI
                     VTSSTPPALDLPGSGGRYTWDADFSDNNFQGLYTVVMTFTFPGWGDCILTGSVANYTP
                     TPSNTPTITPSPTITRTPTLTPAPSPVPTSTVTLPPTITLTPSRTPSPTITRTPTNTQ
                     PATSTPTKTNTPTITRTPTGTFTPTATATRTATPTNTATPTKTNTPPPTLTPSKTPTK
                     TPTPTPTFDPDA"
     gene            complement(78188..79846)
                     /locus_tag="PL000_01930"
     CDS             complement(78188..79846)
                     /locus_tag="PL000_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87680.1"
                     /translation="MIKKLLKKSERGQAIILIAFAFVGLIAMIGLMTDGGVLLIEYAR
                     LKRGIDAASVAAAQQFRKDFTGDDLVSAAENFLRLNQSDVVDVSITMCDPTNAYGGSP
                     AHDPTLCPVGWDTPDTSDDQPARKLVRVTATRNVDFGFMRVLGINGTTIQATSVGEAA
                     SIDLVLIMDTSLSMTYGTTGAANKFDIPGDNPRDCNLVANGGAGACQPLDNIKTVARS
                     FVNNFLFFPYDRVAVVTMTSQSPGGTRDPILYPFEDDVTEADNLLANIKAYEPPICDV
                     DPSTANKWFSPSKGLCLYYPNDDPANTFAGLQRPVYTGGADLNAATSGDGEGNPTTIG
                     SSNIGGALYYANNAFITSGRNDAFWVVIALLSGPANATSTDANFLLTHPDGFCPSSTW
                     SAPKTAPCRDSDLAADGITPLITRHSRGNPDAVPPVPPDANYDADDYARDAADDLANP
                     ETGNGITIFTIGLGTDTGLVNSIYWAGLDSKADTNYKNGLPYSAANLLTYIAEEAGDV
                     YDPVTGVLVREANHGQFYYADDPGLDLGPIFLAIAGNIFTRISQ"
     gene            complement(79865..>80274)
                     /locus_tag="PL000_01935"
     CDS             complement(79865..>80274)
                     /locus_tag="PL000_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87681.1"
                     /translation="LNADGTDDMDYYITTAELVRTELAPPTDGRKLILDPATDGVIVT
                     VYCVVGGTTATRYPDTGPYNTNPSMITSAFTTADIENTYADTAPDAGLVLVEVYYGYR
                     PVIGLFYTGKVMLRAHSIMPANAADPIPGTGCQ"
BASE COUNT        19089 a        19166 c        20886 g        21033 t
ORIGIN      
        1 catcatgaca aagatactta tcgttgacga cgaaccgtcc attgtgaact tggtgcaagc
       61 gtatctcaaa cccgaaggat atgaagtctt cactgcggtg gatggtccct ccgggctcaa
      121 ggcggcgcgt gcttttaaac ccgacctgat cgtgttggat gtgatgctgc ctggtatgga
      181 tggcttggaa gtactttccc gcttgcggcg tgagtcgcag gcgtatgtga ttttgctaac
      241 ggcccgcact gaagagacgg acaaaattgt gggtctttcg atgggcgcag atgactatgt
      301 caccaagccg tttagtccgc gggagttggt ggcaagggtc aaggcggcga tcaggcgcat
      361 tcaaacagga acaggcccca gcgcggaagg aagcgtgctg gcgtttcgtc atgtgcgcat
      421 tgatacaggt gcgcgtcaag ttacagtgga cgatcagacc attgatttga ccgccagcga
      481 attcgacctg ctgcatgccc tcgctgagaa ccgtggacgt gtcctcactc gtgagcaatt
      541 gctggaacga gtgtggggtg gttcatattt cggtgaactc cgtgttgtgg atgtgcatct
      601 tggtcacgtg cgccagaaac ttggcttgcc cgatgtgatc agcacagtgc gtggtattgg
      661 atatcgcttc gacgatgaaa cggcataaag aattgagcag gctatgaaaa ttctgcgatc
      721 ttatcttgga attaaattat ttctttctta tttcgtggtc attttggtgg ggatgaccgt
      781 cattggaatt actacaaaga tcgttacacc aggcgctttt gcgcgtcatc ttttggcaat
      841 ggaaaatcag attgaaatgg ggcagggaat gggacaaggg atggggcagg gcatgggcca
      901 gggtcaaggt cagggcggtg gaatgatgtc ggagttctac cgcagttttc aggctacctt
      961 taccgaggcg ttgctgattg ccgttgtcgc ggcttcgctg gttgcattgg ttgtcagctt
     1021 tgtctttagc cgcagcatcc tttcccctgt gtatgtgatg atgaccgcca gtcagcgcat
     1081 tgctgaaggc cgctatgatg aacgggtgca actgcaaggc accgatgaat tgaatcaact
     1141 tgcgggcagt tttaacaaaa tggcagaaca gttagagcag gttgaaaaca tgcgccgccg
     1201 tttattgggc gatgtcgccc acgaactgcg cacaccgctt accgccatca aaggctctgc
     1261 tgaagccctg atggatggca tacttcctgc ttctgatgaa acctatcaac aaatccatgc
     1321 agaagcagaa cgcttgagtc gtttggttga cgacctgcaa gagttgagcc gcgtagaatc
     1381 tcgtgccatt caattggacc ttcgcgccgt agatgcgact gttctgatcc aaacgatcgc
     1441 caaacggttg caatatcaat acgatgaaaa acgaatcaat ctcaccctca acctgccagc
     1501 tgaccccatt catgttcttg ccgacgaagg gcgcgccatt caggtactca ctaatttggc
     1561 gggcaatgcc ctgcaataca ccccagaaaa tggaagtgtg accatttccg tgaagaaaga
     1621 gaaagacgaa gcgcaatttt cggtttacga tactggggca ggtattcctg ctgaacacct
     1681 tgcacacatc tttgatcgtt tctatcgggt agataaatca cgctcccgtg cgcatggtgg
     1741 ttctggcatc ggtttgacca ttgccaggca tcttgtcgag tcgcatggcg gccgaatctg
     1801 ggtggagagt gcaggaaaaa ataaaggcag tgtcttttca tttacactgc ctctggctaa
     1861 gtaaagatcg agtttactga atgggtttca ctacaccgat atctgacggg gtgccgccaa
     1921 aaagactgat atccgtacag gattgcctat cgacacgcag taacaacaaa tcgttggatg
     1981 aagcatcgac ttggataatg gtgtctttgt tataggctga gtcaacggtc agtttgagtg
     2041 tcacttgatt ctgtatgggc aggttgggaa cacaaacttt tttatcagga tggtcgcggt
     2101 cttcatcaat ggcccgcagc aaagcgcagg tgttgggcag gtccgttgtt cctgtattgg
     2161 tcacggttac gtatgcattc gttacttcgc ccattccgtg aaggatgtca atgctggtgt
     2221 tgcagccaag gatggtcact gttaaggcct ggataggtgt tacaggggtt tcagtgggcg
     2281 tgaatggagt aaatgtaaaa gtggggacgg gggatggggt ttcagtgttt gtagcagtgc
     2341 ttgtaacagg ggttagaacg atcacccccg gggttaggat gatgggtgtg gctgtaatga
     2401 tgtttggaag ttgggaagga acaggggtcc acacggggag cggctgcggc aggttccccc
     2461 aagtatcgca ggagctaagt gtcagcgcca aaaacaggag tgttaatatt ctcttcatca
     2521 tcctggtcat tatactcccg tgttcagcat ataaccaaaa gcgccgccaa aagcaattcc
     2581 acttttgacg gcgctgaaga gaaaaaatcg aactataggg tttctgttgg gatggcggtc
     2641 tttatccgta actcttccca acttggttcg accagcacgg aaccgtcagg aaagatgata
     2701 gttggaacgc ttcgattgcc atcgttgatg tccatcacga aaagggtggc ctcttcgttg
     2761 ttctccaacc caaccttcaa gtactcaata ccatttgttt cgaaaaactt ttttgcccgg
     2821 tggcagtctg gacaatactc ggtggaatac atgacgattt gagatggggt tttattgtaa
     2881 agatcgttgc tcacagaaaa atttcctcca ggatgaattt gttcaatgcg tttgatgtgc
     2941 aagaggaaaa gattcttacc acttaacgaa accgtcatgt ttacaaatca atataaaggg
     3001 taaaatttgg gctgaatttg tccaaattga aaaatgagat attgttcttt ggataagggt
     3061 ctctggaact atggagtttt cccatttcca ctttttcgaa ttgattaata gcctttttat
     3121 gctaaaatca agtaatgaat tatttaatat gaaattgata tttttgttag ggtaatcacg
     3181 tgacaaacct caaaagatct gttttatcga tcgcattttt cttgttcggt atattggtgc
     3241 tgggtcagtt agaccgctcc gatacaccga ttattaactt tgcatcttat ttttatttgc
     3301 tggcaatttt tattgttcca gtgatggtct ttgtgccgtc gctccacaaa atttcggtgg
     3361 ttgtgccaat ggttttttgg ggcgcagtct attttggaat tttccagctt actgatcgcg
     3421 gcaataccgg gtcgttggat gtggaggttg ttgtcctcga actggtcatc atagaagtgg
     3481 gcgtttggct ctccttccaa ctcgcacttg ggattgaacg atcggaatcg ctgatggatg
     3541 ttctggcaca agggacattc cctcaccgcg cacttgaaat tgaccttgca aacgagcaga
     3601 tcaaggttga gttcagccga agccgtcgat atcaccgacc actctcgctg cttgtcttgc
     3661 atgcttttcc acaagatgaa gcagttgttc gtgagatgtt aatgagtttg cagcgcgatg
     3721 tgcttacccg cctgtctaat gcacgagtcg gtcaggcgat cggtgaatcc atccgccaga
     3781 cagaccttct tgtccgcgac aaggtaggca ggtatattgt tttatgccct gagacagatt
     3841 tggagagtgt gtacctactg gcagaccgtc tttccaaaat tgttgtggag cggacggggc
     3901 ttcatgtaaa ttgtggtgtg gcctcttttc ccgatgaagc tctgactttt gaagatttgc
     3961 ttcatctggc acgtgatcgt tccaaacagc ctttaccgtc caaggcagcc attgaaatga
     4021 aagagcaggc tgttaaatga gcagcacatc catcaaccct gccgaccagt attcctggat
     4081 tgcttctttt gacccaaaga aacgcatttt tgtagggcgt tcttatttta ttttaaaacg
     4141 cataatggat ttatctctgg tgctcttgag cgccccattt tgggctcctt tattattggt
     4201 tcttggaatt attatcaagg ttacctcgcc tggggcacca gcgttattca cgcaattccg
     4261 aactggaaaa aatggcaagc gttttagtat gtataaattc cgttcaatgg ttccgaatgc
     4321 tgaggcttta aaggaaaagt acgctcacct gaatgaattg cagtggccag atttcaagat
     4381 caccaatgat ccccgtatta cgaagatcgg aaaaattctc cgaaaaacaa gtcttgatga
     4441 attgccgcaa ttgatcaacg tgttgaaagg tgatatgagt ttggttggtc cgcgcccgac
     4501 ttcctttggc gctgaaacct acaagttgtg gcataccgaa aggttggatg taaccccagg
     4561 tctgacgggg ctttggcaga tcattggccg tgcttcgatg gaatttgacg atcgtctgcg
     4621 cctggatatt gcctatattg aaagacgaag catttggatg gatattaata tcctgttttt
     4681 tacagttttt gccgttttca aagcgcgcgg ggcacattag cacagaatcg aaaaaaaaag
     4741 agacgctttc gagcggcagg ttttgcctac ctattcagag gcgagcttgc cgcttttgct
     4801 atatgatgtg ttctaaaaat ttctttttcg agtcagcttt tatataataa aaaagactac
     4861 cctgtatcag ggtataattt caatctcatt ctttccacat caagctgata ttgttctact
     4921 ctactcacta tgaaaagaat tccttttttt attatcttgg cttgtctatt atttacgagt
     4981 tgtactcatg gttcagaatc cacccccgtt tcatctgaac tgggggggga ctttgataat
     5041 gggggtgatg tggtttcgca tcccaactct tccgaaaatg ccgatgtaga ctgtgacctt
     5101 ccctatacca aagattccat ttggaatatt ccaattgact ggtcaattgc gaaaattcat
     5161 cctgacagcg acaaaatgat ggaggcattt tgggatggaa gccgatggat cggttctgac
     5221 ccaacacaat atgctcctaa tatctatttc gttgataata aaacgccatt ggtgcctgta
     5281 aaacttcgaa aaaaccgctt tcgtgatgcc tttgatgata aagaaattca gtatggtgaa
     5341 cccgctgcct ctgtatggat gcctattcca gagggggcac aacctgctcc aggaactgat
     5401 gggcaaatgg tggttatcaa tgtagatact ggtgaggagt ggggtttaaa caaggggaca
     5461 attgatccct tgggaagttg gtttgccaac ggaatatacc gttatagtat tgagaattct
     5521 ggagttcccc cggagggctt tggtcagcgc ggggctggga tcgggaattt ttctggaatt
     5581 gtacgaaaat gtgaagttga cctgggtgtc attgagcatg ctgtaacact ggcttatgat
     5641 tttccatgta cacctgagac gtgtggtgcc aatgggcggc ctgctttcat cccacctttt
     5701 acgaaaaccg acggtagagg cacatccacg tatgacatac ccgaaggtgc gcgcatgatc
     5761 attcacccgg aaataaccaa agaagaaatc gataatgcat gttcgggtat gaagggttgt
     5821 atcgtttggg tgcttgccat gcagaaatat ggcggtttta ttgttgataa tagcaatcat
     5881 ccgaagacct atcctgaagg agaggctacg gcaaattggg atcctgaaat atggtctgat
     5941 gatatgttac gcaatattcc cacggaatgg tatgatattt tggattggaa ctacccgtcc
     6001 acaaccataa agtagtcaag ttgattttta gaggatatgc atatgattac ccgccttaaa
     6061 aaaatagaga aaagtgatgt cgtgttcttt gtgttaagca ttgttttggc tgcagggagt
     6121 gctcttgcca ttggcaacct tgacagtgac attggcttat atggtttggt gggattggtg
     6181 ggtattgctg tggctttgac catgtttgtg atgccaagct tcggcgcaaa tgtcctgatc
     6241 attgccgtgt tttcaaatgt gtccgatgaa ttgtcaaaca atggctaccc cggtatcatc
     6301 aagcctcttg tagtggttgt ggtggctacg atcgtcgcgc gcttcattta ttttgagaaa
     6361 ccccccgctg cccgttcgac aaccacgaga attgaggcat ttctttttct ttattttttg
     6421 gtttatgcgt tttctttcat gatggctgca gacaaatcgc gtgcatcctc ggtgattttt
     6481 gacgtagcca aggatattgt cgttatttat tgtttaatgt atacactaag gtcccctgag
     6541 gccttgaagc gggctgtgtg ggtggtgatt gtagtaacgt ttatattgtg ccttttgggg
     6601 gtctaccaat ccatcaccgg caactacagc aataattttt ttggctttgc catggtaagc
     6661 cttgatgact tttccggctc aactgcagcc cgattgggtg ggccgattaa tgcccccaat
     6721 atgtggggcc aaactgtggt ctctgtgatc gcccttgttg cttttcgtat tatccacgaa
     6781 aggcggatga caacacgatt attctccatg ttggttcttg gtgtttttct ctttgaggtg
     6841 ctcaacacct acagccgggg agattacctc gcgttggccg ttgttgttgt tttgatattt
     6901 tttgtgtttg aggataagtt cagtcccatg atagcattcg ccggggttgg attgatcata
     6961 ttgttaattc cgtttattcc ttcaagctat acggagcgct ttgcttcact aagcatctta
     7021 acctcaggag atcaaagcgg catttatcag gaagactctt ttagaggtcg tagtagtgaa
     7081 cttttgaccg ggttaagtat gtttaaggca agtccatttt tgggggtggg tgcaggtaat
     7141 tacaaatata attaccagaa atattctcaa ctgatcggta ttgagcaacg tgctgaagcc
     7201 cgtgacccac attcccttta tgttcaactt ttggctgaga atggaatatt ggggtttctg
     7261 gcttttgtgg cggtgattgt ttcagtgttt agtggtttaa ataaggtgaa acgctccatt
     7321 gattatttgc ctgcattaag agagtcgtgg gtgccatgga tcagttccct gcaaacttcc
     7381 atggttgggt atttggttgc tgccacattc ctgcatgggg catatattcg atatttttgg
     7441 atcttggttg ggttgtcgct taccgcaata cggttgacag atgagatggt tgcggaaacc
     7501 aatcaggccg ccctttccaa aacgacagtt taatttttac cgtctagatt tataattaaa
     7561 taccaagccg ctttgttaca ttggcaataa aatgaggcag ggcggcaact ttcggcgctg
     7621 aaaaagactg ccagttataa agcctggcgg tattgctatt tttaaaggat ggttttatat
     7681 ggatgcaaaa agaatgatgt caaaattacg ttcattgcct gctttggttt atgaaaattt
     7741 tctggttcgc agttatcata atttgtttgg aaaacagata aatgataggt ttgtcatttt
     7801 gggccacgcg agaacaggtt caaattatct ttttgatggc ctgataagct cgaaatccat
     7861 caccggctat catgaaattt ttgctgatca caaccgtgcc attggtaaag attttgagaa
     7921 agtgctctcg caggtatacc gtaaggaaag caaggataca cagctggtgg gcttcaaact
     7981 tttttacaac cacctgaccg acgaagagtg ggctaaattt ctgcagcatg aggaatttag
     8041 gattattcac ttaacccgtg caaatcgcct ccgtacgatc gtttccctgg atattgcctt
     8101 caaaacagat gaatggacac gctcttcccc ctccaaaggg aatcaactgg ataagaaacg
     8161 gatcacgctt gacacctcca aactgatcgg ccgactggag aagattcaag cccaggagga
     8221 gttggcccgc aagcgatttg ctggccgtcc catgcttgag gtggtttacg aagagctggt
     8281 tcgaattccc aaggtggtat ttaagcaggc tgaagacttt ttgggcgtaa atgacataga
     8341 ctttaccaag ataaagataa tgaagcaaaa cacggaaaaa ctggaacagc tgatcgttaa
     8401 ttacgatgag gtttctcagt gcttgaaaaa ttcacaattt gcttcctgca tggatgagta
     8461 aattatcaag atgtttttca ataatatccc tacagttcgt gattttattc cattcttatt
     8521 gagactttat aatgactgat gaatctgtaa aacaagatgc tggcagccag ttctcgcgca
     8581 aagtggcggg tgggcttctt tggaattccc tcacttttgg gtttggcaaa ctcgttgtgc
     8641 tgttaaccac atctgttttg gcacgtcttt tgacaaaagc tgaattcggt caggtggcgg
     8701 ttgccgttgt ggcaattaac tatctggctg tgatcaaaga ccttggattg ggcgtagcct
     8761 taattcaacg ccgcggtgaa gtggataatg ccgccaatac ggtatttacg ataaatacca
     8821 tgcttggctt ttttatctcg gctttgataa tcccccttgc gccacccatt gctgattatt
     8881 ttcgtgaccc cggggtagtg cccgtattac gatggcttgg gctttccttt gcgatcaatg
     8941 cgcttgggtc ggttcatatg atctggctgt tgcgtgactt ggattaccgc cgaaaattca
     9001 ttccagatat gggcaataca gttgtcaaag gaattgcttc tatcggactg gcgttcgctg
     9061 gatttggcgt ttgggccatg gtgatcggcc agattgccgg ggcattgact tcggtgatcc
     9121 tggtttggat cattttgccg tggcggcccc gtttgatgat cgatcgaagc attgcggggt
     9181 ctttgatcaa atttgggtca tccatcattg gcggagacat ccttggaacg atcattgata
     9241 acattgatta catcattgta gggcgcttgt ttgggcttgc ccaactcagt gtgtacaccc
     9301 ttgcataccg cttgcccgaa atgcttttga tcggcaactt atgggttctg ggcgagatcg
     9361 cctttccagc tttctcttcc attcaggata agcctgaaga aatgcgcaac ggctttcttg
     9421 gctctgtgcg tctggtggaa ttgatcgcca ccccgatctc ccttggtctc tttatcgctg
     9481 ctgacccgat tatcagggtc gtgtttggtg aacagtggtt ggaagccatt ccgatgctga
     9541 gggttctggc catttatgcc tgggtgtatt ccattggttt tcatattggc ggtatttaca
     9601 aggcggtcgg gcggccttac attcttttca acctctctgt tttcaccttg atcattttgg
     9661 tccctgcttt gttgatcggc gcccagtttg gcgtgatcgg cattgcctgg ggacatttga
     9721 tcgctgttct aattcgcaga gtggtcagcc tggcagttgc tacgcggttt gtgaatgtga
     9781 ccctcaaaga catcttcagt caattggtgc catccttgcg ggctggcttg gtaatgaccc
     9841 ctctggcatt tgcagttctt tatgctgctt cggggcttac tcccattgtt caactgatat
     9901 tggttgtgat caccggggcc gtcagctacc ttggcgtggc atggtggacc gaaaaagaga
     9961 atctctttcg cctgatgaag gcgatcggtg gttcaaagaa gaaagcgtag aggaaatcta
    10021 aaatgcaaaa tcatcgtttt atttttgtca gcggattgca tcgcagcggc acctccattc
    10081 tgttcaagtc actgcgtgac catcccgaag tgagcggctt ccataacacg aactctcccg
    10141 aagatgaagg catgcatctt cagacggtat acaaaccgtc ggggtattat ggcggcgctg
    10201 gaaaatttgg tttcaatccc gaagcgcacc tcaccgaagc ctctccgttg gtaaccgaag
    10261 aaaaccgcaa aaaattgttt gccgaatgga ccccgtattg ggatatgagc aaacaatact
    10321 tgctggaaaa atcccccccg aatctgattc gcacccgttt tttgcaagcg atgttccctg
    10381 actcatattt catcgtcatg atgcgtcatc cactggcggt ttcttatgcc acccgtgcct
    10441 ggtacaaaaa ataccgcatc tactggcgcc gcctgccgcg aatctttgag cactggctgg
    10501 tttgccatga gatctttttg gaagacaaaa agcatttgaa gaatgccatg ctcctgaatt
    10561 acgagcagtt tgtggcagac ccttccactt acaccaataa gatttatgaa ttccttggtc
    10621 tgagcccctt cccgaataac cagaaggtct tatccagtgt gaataaaaag tacttttcca
    10681 tgtgggagaa agaccagaaa ggtttccttt cggggttcat tgcccgtcgg atgatcagcc
    10741 gttttgagac ccgcctcaac cgattcggct acagcctgct cgatgtagag cgggcagacg
    10801 cgctcgccaa ataggcatga attctcaaag ttccccgagt accgccgcgc acatttcact
    10861 ccccagtcat ggggttgatt ggcgttttct gcttccattg cgagaacaca cccgcatgtt
    10921 ggtgattcat ccatcggatg ccgactacga ccgctctttt caggaatttg gaattccctt
    10981 cgaaactgtt tctttcgaca acttcaatac ggatcgctcc ccccttcaat ttgaatcggg
    11041 gagctttgat attgttgcgg cgcctttggg ttttggcggg gataatcgtg cggggaggca
    11101 aagccagttg gggaagaacc tgttagcggt tcggagtttg attcgagcag gtggcatgtt
    11161 tttgctggga ttctccaatc gctgggatat ccgccgcaag ggtcgggaat ttcccaattc
    11221 ttccactacc tggagaatat cccgcagttt ggaaagggct ggttttgatg gaatgcgttt
    11281 ttatgggact ttccctgagc cgttgacccc tgaatatatc tttcctttga attcacaatc
    11341 acttgctttc gttttgaatt acagatacca gcataaattg cctggttatt ttttgaagtt
    11401 gaccagaacg cctgctgtga acatgctgct gggttttgtt ccgttctact atgcggttgc
    11461 cagtgtggag gcgtgatggc ggcattgatt gacgatctca caaccctgct tgtttcgaac
    11521 agccgtcgtt cgattaacag caatctgaat tgggtgatgc tttcaaacgg agcggccgac
    11581 ccgaatgaca atgtgatttt ctttggtttc gaaggaaaag caaccgtgcc ttcatttgtt
    11641 gccaaagttc cacgcctgcc ttctaatggg cgcatccttc gagaggaata cgatcggctg
    11701 gcggaggttt gggggctttt gggtaaagac gcctctctgc ttgtacctga accgctcgca
    11761 ttttttgatg tcggtcagca gcaggtttct gtgatctctt tcgttcgcgg cgatgggctt
    11821 ttgttttctt cgaaaaagaa attatggcat gatcccattc aggtgctgaa cttatctaaa
    11881 acggtggcgg gtgctctgcg agaattgcac gaaaagacgg ccaaagaagc tgcggatgtt
    11941 gtaagtatcc cgacggattt ccttgaaaac cttgagctct tcaagaagat ttacacaccc
    12001 acagcagaag aatcactcgc cttttctgag ttggagcagt attttgcgcg ccactcagtc
    12061 cattatcaaa ccttgcttca tggtgatttt tggcacggta atatcatccg tagttcacaa
    12121 catggcacgc tgatatttgt tgactggcag cattcacgct ggtcgagtga tgtcagtgcg
    12181 gatgtgtaca tgtttctgat ggcgggagct ttgtcggctt cgcggggttc acctgaagag
    12241 cgggcaagcc tgacggcaaa actccttgtc aactggcaac aggagatttt ccctgcttat
    12301 ctggctgcct atgggcccac ggagcgttac tcactgcttc cgccccgcct tggaatgcaa
    12361 gtatgctgta ttgaaaaggc agtgcggtcc atacgtgatt ttggttataa tcaatttgat
    12421 gatttgatct ggcgtaatct tttgaccgaa ctggtcaata tcccaaaaga tggatttctg
    12481 gatggcagct aacatttccg cgaaacgcaa atacaatatc atctttgtga tcgacggtct
    12541 gggcatgggg ggggcggaac gtttgatggt tccaattttg aaaaatctga gcagggaata
    12601 ttttgaaccc cgcgtatgtg tgctgcatgt gaaggatggc aacccgatcg cggaagacct
    12661 gcgcgctttg ggggtgcccg tggacttgtt gctgattccc tatttgcggg atgttacagc
    12721 cattccccgt ctgcgccaat atttgaggga tgtaaaagcc gatcttgttc atacacaatt
    12781 ggaaatggcc gatattatgg ggaatcttgc cgcaaaatcc atgcgcctgc cgagcgtctc
    12841 caccatgcat acaatgcctt cgcaggatat gaagctcaaa tcgaagcttc accagactgt
    12901 ggaactttta gccctgcgcc atttctgcaa caaggtcatt tctgtttcag aggaagcccg
    12961 aaaattttat ctaaagatca gcaatctcct gcctcaaaac ctgctgacca tctacaatgg
    13021 cattgactta agccattttg aacacctgaa ccatcagcag gatcgtgtaa agatccgcga
    13081 agaatttggc ctgcctgctc aggcgaaaat cctcatcaca gtggcagtgt tgcgcgaatt
    13141 caaagggatt caatatatga tccgcgcttt gcctgctgtg gttgccaaag accccgatgt
    13201 ttattatctg gtggttggta atggaacgta tcgcgaagcc ctcgaaaaag aagttgaaca
    13261 ggctggcatg aaagaccatg tggtatttgc aggtcagcgc agcgatattc cgcacttgct
    13321 ggctgccagc gatgtctttg ttttaccgac actgacggaa gcccttccca cggtgttggc
    13381 tgaagccatg ggtgcgagct tgccgatcat tgccagcagt gtaggcggtg tgcccgagat
    13441 gataaatgaa ggcgagaacg ggttgctttt gtctgcagga gatgttgacg gcctgatatc
    13501 tgccagtgcc tcgctaatgg ctgacgaggg ccgccgtaag gccatggggg aacgtggcag
    13561 gaatattgtc aatgagaaat ttaacatcca tgttcaagtg gagcgattga gagaccttta
    13621 tctgggtttg ataaaagaat atgaaaaata aactgcgcat ttttgtcgtt gaaccgaata
    13681 acggtggcgg tcttgtccat tttgcctacc agttgtgcaa tgcattggcc agtgaaggtg
    13741 ctgacgtcac tttggttgct ggggcaaaat atgagatggc agaccaaccc cacagttttc
    13801 atgtggagaa gttgctcaat ctttgggaac actttgaccc cgattcgatg aaggccgaga
    13861 tccagaatcc gctgaaacgt gcatggcgga aaatttactg gaccctgcga cgaatggccc
    13921 gtggtgtccg tttgatctat gcctgggcca gcttgaccca gtttttgttg cgcgaaaaac
    13981 ctgatatcgt tcagttttca aaatacgaat attcctttga gtcttatttt gtcggctatt
    14041 tgcgccgccg tggtttgatc gtcactcagt tgtgtcatga atttgagttg cgtgaaagtc
    14101 aggactttat ctggtcgatt ttggatcgta tcagcggcga agtctatcgt catttttcag
    14161 ccatcttctt cctttcggaa gagattcgca agagatttct atccctgcat tccaccatcc
    14221 ccgctgaacg aactttatct gttccacacg gtaattcggg ttggttgttg aatatccccg
    14281 ctgtttcggc agacgtgctt cgtcagcgtt atggattgca aaaagacgag cgtgtggtgc
    14341 tgttcttcgg attgctggct ccaagcaagg gattggatga cttgcttgat gcttttgcga
    14401 ttgcgcgtaa gacctgtaat gccaaactgg tgattgcagg ttatccgacc aaacatatta
    14461 gtatgagcga gatgcttgag aaaaatcgaa atctcggcat tgcagaccag gtgatttttg
    14521 atgctcggta tatcccgctt aatgagatcc agccactaat ggcattggca tctgtggtgg
    14581 tgtatccgta tcgaagcggc acacaaagtg gtgcgctgca aactgcttat acgtttggaa
    14641 aacctgtcat tgcaacagca gtgggtgggt tgcccgaggt tgtggaaaat gggaaaagcg
    14701 gatacatcgt ttcgcctaat tcccctgctg aattggctga aaagatcgtg gccgttgtca
    14761 acgacccgaa gctgggcgat gaaatggggc aatacgcccg tcatctttct gagactcgtt
    14821 ttagttggaa aactgttgca gttcagatcg tggaagtcta tacccgtttg cttgaggcaa
    14881 ggaaacgggt atcaaactaa tttcgaagac gattaaatct gcttgtaatc gtggcgtact
    14941 gtgacaggga atgggcgtgt gaaataaaga cccgccgtat tgtgaacgga ctttgctgaa
    15001 cttcctgcaa agtagtcggg ccgttttgca gcgtgaacgc cactttatac cctgcgtcag
    15061 ccacaatttt ttgaatatcc gtgttgtaat ctgtctgcat gccattggga taggcgaaac
    15121 taagaatggg gcgaccaagt tccttttcga tcttggcttt ggatgtttca atttcagagc
    15181 gggcctgctc aagtgatatg cgggtcaaaa tggggtggtt gacggtatga ccgccaaaat
    15241 cgattccgtt gtttgccatt tcccgaacct gatcccagtt catcattaaa ttgcggaaaa
    15301 aattatcggg aatggaaacc ccaagttgtt ctggcaattg atttacccat ttgcgttttt
    15361 cttcctctgg tagaggcttc atggcataga tccaactttt ggcgatctgc cagcgttcaa
    15421 tgggattttt ccagaagagt tctttcttat cgggaaataa aacatgatct gtttttgtgt
    15481 ttagaaaaca gtaggcggcc aggtcccaat aaaacggctc gtttgtatcg atatgtccgc
    15541 tggtcaaata aatgacagct gggaatttat atttcttcag aacaggatat gcattggtgt
    15601 agttatcaag gtagccgtca tcgaaggtga tcagagccgc atagggcggg aggggttttc
    15661 cgccagtgag ccacgtcact acatcttgca gggagaccac gttgaaccaa cgggagagat
    15721 attccatttg gcggtcaaac tcagcgggat gggcgctaat gtttggctga aaggaatcga
    15781 aatctttgcg atgcgggtca tcaatgcgat gataattaag tacggtcaac gacctggccc
    15841 aaaactgcct cccgattctg atgagacctg tcgccgctgc ggcatcggta aaaactttca
    15901 tcaagccaga tcgcgaaaat ttcctggggt tggttttatc tgaatacatc aatttcttcc
    15961 tgcaataatt attttgaggt tatttttttc tttttgcatc atgcctgtcc agtgcttttt
    16021 ggtaggcatg aaaagtgttt tggatttgag tatccaagtt aaagactgtt cgtacccttt
    16081 gttgggcatt gtctcccagc ttttgggcaa acttcaggtc tgagcataat ttgcgcaggc
    16141 cttccgccag tgcggcaggg ttgttgggtt ccaccaacaa ggcgtgttcc tggtttgtaa
    16201 ccagttcggg aataccccca gcggcagcgg ctatgatggg cagccccatg gcagctgctt
    16261 ccagcagggc aatgggcgtc ccttcgtagc gtgatggcat tgcaaagata tcgctggact
    16321 ttaagatcgg catgacgttg ttgcggtctt ggtatcctgt taaaagcacg cggtcttgaa
    16381 gccctgcatc tttgatctga ttggagagtt cttccataca ttcgccttca cccacgatca
    16441 aacaataaag ttggggaatt tgagcagcaa cctgcctgac cgcctcgatc aggaggtcat
    16501 atcctttaac tggaaccagg cgtcctacgg cggtacagat caaggattgt ggggggaggt
    16561 tgaacttttt acgcaggcca tcagggtcgc tggaaatttg agagtcgtca aagctgacgg
    16621 cattgtagat taattctatt gactgggagg gaatgcctga tttcataagc gcctgacggt
    16681 ctttttcaga tacggttatg tacaggtcga gattgcgatt ggtgaaaagt tcaagtgcgg
    16741 tatacatgcg ccccttgagg cggtctttgc ctgaactttg tccatgctcg ctggcgtacc
    16801 agctattgat ggtcgaaacc agggaagtgt tcgttatgcc agcagcgagg ctgccccaga
    16861 atttggattg gatattctgt gtgtccagca cctgaaaatc tttttcacga attaagcgga
    16921 caagccgcga tacgatccgc gggtcggttt tctttgcgcc tactgtgtaa acaggcaagc
    16981 cttctttttg cgccgcgagt actactgcac cttgatctaa acctgcaaga gcagctccct
    17041 gatcggccag ggctcttaaa atgccaagca cacgtgaact gcttccacca tacccaacgg
    17101 ccagatcaat gataagcggg cgacaggttt ccatttttta ttgtctcttt gcctgttgcc
    17161 aaagaacagt gtgttgtccg gagaaatacc aaagcaggcc agcaatgctt gcaaaattcg
    17221 ttgcgcatag gaaatatggg agcatgaaaa ttttgaagac ttttgatttt ttgagaggag
    17281 tgtttgaaag aaccgctccc aaaagtgcca acccataaaa tattccttgc agaatggcca
    17341 gtgcaatgag catggcattt gccagaggcg aaagcgataa ggcagggaga agtgccacaa
    17401 gaaggatatt tgtcagcaag gccagaatca tcaggtgcgg aatagccagc cgcaggaatt
    17461 tatgagaaat cacttgaaaa cgaagcaggc ttgataattt tggaagatat tccctgctca
    17521 tgaacaccat ttgatagcgc cctgcagtta ggcggcgacg gcgattgatg tcgtctccca
    17581 tggtgttggt tgggcgctcc caaacaacag atttcggttc tgagatgaca cggtaaccct
    17641 gactgatggt catcatggcc agatagaaat cgtccagaat tacattggat gggatgggtt
    17701 gcatgatctc acggcgcaga gccattaaat ggcctgtagc agctacggtt gagtgaagat
    17761 cgctttcctt ttgacggatt aaagcttcgt agcgccagta caatccacca gattcgcttg
    17821 tttcctgttc attggaagac atcatccgag tttgtgccac cacgcagcca actttttcgt
    17881 cggaaaaatt tacgagtaat tcgcgcagtg aattttgatt taggatggtg tcggcatcgg
    17941 tgatgacgta gatctgcccc gttgccagcc caagcgcccg atttagcgca cctactttgc
    18001 ctgctctggc cccttcactt agttgaatcc ctttggatgc atatgtgcgg atgatctcgg
    18061 ctgtgcggtc atccgatgca tcatcgcaga cgatgatctg cagtaactca cgagggtaat
    18121 cgaggctcag gatgttatca agtttttggt tgataaccgc ttcttcatta tgagcaggga
    18181 tgacaatcgt ggcggtgggg agggtgcccg tttttacgat gggttgtcgg cgatattttg
    18241 agagtacaac aatgatcagc gggtagccca catagatgta aagcaatagg ccaaggctga
    18301 ttacgagtaa aataacaaga atgaaggcga gggaaaacat gttgctccgc gatgtgatgt
    18361 agtttttagg aaaaatggat tacttgcgcg tgcattgcaa tcattatatg ggtaaagtac
    18421 ccctgtttga ggctatgtta taatttattc ttaaattgta acactaaaca attagcttat
    18481 tggagagttc gttatggaaa ttcgttttta ttttaaaatc atccagcgtg ggtggtggat
    18541 cattctggtt acaaccctgg ttgcgatcaa tgtttctctt gtgtattcat attactttac
    18601 tactccaatg tatgaggcgg tggcacggtt cattgtgagc cccaacatgc agaatacgga
    18661 aagcagagac ttggttaata gccttgaggc attggataag cgttccatta tttccactta
    18721 tgccgaagtg ctcaacagcc atcagatcat cgaaagcaca atggagttgt tgcgtgaaag
    18781 cccgaatgat tttaggggct attcaacttc tgttactgta gtgcctgatg caaatattat
    18841 tcgctttagc gtgaaaggcc cagacccgga tgtggctgct ttgctggcca atagcatcgg
    18901 tcagcatgcg atcgattatg tgaagaacct ctatgtcatc tatgacattg attttcttga
    18961 caaggcgttg gtgtccggtg acccgtatca gcctcagccc gcccaggatg ctggccttgc
    19021 tctgatggtg gggttggttt tgggcgtagg tttggcgatc ttcaaggatc agattacggg
    19081 gacaattggc aagctgggtg aacgcaatat gctggatgtt gaatctacgg catttacacg
    19141 ggcgtatttt gagcgacgga tgcgtcaaga ggtgatcaac cagccatcaa gcgttttgac
    19201 gtttggcgtg attcaccttt tgggtattca ggagatttac gactctctgc cacaggctta
    19261 tgtaaaccag attttgcgca aggtgactga aactttgaag tatcaattgc gcggcaatga
    19321 tattgtggga agatggtctc aattggagtt cagcattctg ttgccttcta cagatggtgt
    19381 ttcggctatg aatcgcttgg aacggattcg agaattgctg aatcagccca tttcgctgga
    19441 aggggaaagc gggatcgata ttgtccttga cccgagaatt ggtttcgcgg accgccaagg
    19501 cggcgaatcc atcaacgtat tgatctctca ggttgaaaaa gcgcttgaat tatcaatgga
    19561 gtctgaagaa aaggttaaca tgtacaaggt taggccgttt ggctaaccgt taatcatgga
    19621 tgctggttcg ttctgcgtct gatacatatc gttatcttat ctgatttata taatgtcggc
    19681 tgatgtttgg taaacggagt ttttatgaaa aataagatgt tcctgatttt gggaatactg
    19741 gtgattttgt tgactattcc actgcatgct gctgcaaagg caccagaagt ggatgtacag
    19801 cccgaaaaat atgcggcaat cgagatcaat ggcaacaatg ccaaggttcc ctttttggac
    19861 ttgggctatc gtgagcgaac cctgaacagc ccatatggaa ttactaatat ttatttcaat
    19921 acccccctta gctggaaatt gattcctggt ggagaaattg agatcaacta tgatgttttc
    19981 ctgagcggcg ctgaccttgg caaagttgtc gatagtgcca atccgtatgg cggcaatttg
    20041 atctttacat tcaacggtca gattatcggc tcaacccctc tcgaagggat gggaagccat
    20101 actgttcggt tggaaattcc cgatactgcc ctgacttcga tcttgcagga tggaaggcac
    20161 cagcttacga tttcccttga ggcccaattt agctgtgatt acgatgtcaa tgccagaatt
    20221 gttgtcaacc ccacttcgtt ttttgacctc ttctttgaag aatcaagccc ggaactgaat
    20281 ctttcacgcc tcccttctcc gttctacctt gaaaattcct ttatccctga cagcactctt
    20341 gttgttgtgg ctgataaccc tgatgctttg gaacttcaat cggctttgaa tgtcatggct
    20401 gggtttggtt ccatgatcgg cagggtgtat gacatgcaac tcatcacggt cagtcaattg
    20461 gctggctttg atcccacact ctatcacatg atctttgtgg gtactgcgga taaattccct
    20521 gttctgaccg atgttaattt caaaattccc attacagatg gtaaatttgt aaatctttca
    20581 cctgcctctg aaacggacgg tattgtggaa ttggcccttt ccccgtggaa tcccaccaag
    20641 gtgattatgg ctgtcagtgg gaattcaggg gatgcggtga tgaaagctgg tcaggccgta
    20701 agctctggca gggtcttcgt ttatagtgac ccgacagttg cttttgtttc caatgtgcag
    20761 gctttatctg acacgcttcc aattgttgaa gacttcacct tcgaaagtct gggatacgtc
    20821 actgaaaccc tgtctggaat cggcttaaaa tcgaaggaat acatcttcta tgtcagcaag
    20881 gaacaagtat taaccaagga tgcctatgtg gatctggtct actaccactc cgggctgctt
    20941 gattatggtg tttcctcatt ctctgtagaa ctgaatgggc aggtgatttc cagtacacct
    21001 ttcagcaaag agtctgagca agtgactacc ttgcgaatta aattcccgcc tggaacactt
    21061 cgttttggtg agaatcgcct cgatattcgt gccagcatga tcattttgcc ttcctgcgat
    21121 acgtcaggct tttcagaccc gtggtttact gtttccaacc agtctagcat tcacttgccg
    21181 gtaaccctgc ctggaatcaa tgctgagccc ctgctaaaag acctgaagtt cttcccggaa
    21241 ctttttgtaa cacacagcga tcttggtgat attgctttca ttttcccgaa atcggatgtc
    21301 gcgagttggg tgatcgcagg gaagttagcc tataacctgg gtcagcaatt taatcccttg
    21361 atcgcaaatt tggaagcagc cttttctgat gaggtgcccc agcaggttca tgatgataat
    21421 tcgatgatta tccttggtct tgccagtgag actcctttcc tgtctgagtt caacgacatg
    21481 cttcctgcgc cgttcgatat ggccagtaac cttgccagcg aaaaacagat gcaggttgta
    21541 taccgaattc ctgaaggggt aagcgttggt taccttgagc ttttattgtc tccatacaac
    21601 gctgaaaagt ccattcttgt gatttctggc aatgaccgta acggtgtttt gcttgcaggt
    21661 gacagcctgt tggtgaacga actggaaagt caactggcgg gtgtttttgc agtgacgaac
    21721 ggaaaacaag ttgccacagg taatgcatct tcaccgttct caattgttga taatgttgtt
    21781 ccaggggctg aggcggtcag cactacgcca gttcccgaaa tccttgctgg tggcccgcca
    21841 ttgttcgaac gtccagtttg gctgattccc gccattgtgc tctcctccat tacagtggtc
    21901 ggtgttttgg gatatgtggc gatctcagcg atcgcaaagc gtagacagga aaagcggaaa
    21961 cttctggctg gcgaggaaca aaccaatcag gaagaagata agaaatagca gtgtaactag
    22021 aattaaaaag agcgttgacg tgagatcaac gctcttttgc tttttaatca cttttgtgca
    22081 aaacaggcta cttgattttt tccagactcg cattttccag aatgataaaa cccagatcga
    22141 ccagtcgctt catttcggac cgttgaacgg gtcggcctgc ctcggtcttt tccagcatgc
    22201 ggagcaatgc cccccgtaca ccactggcac gatgtgccca gtatgagtct gcggaaatgc
    22261 gttccaaacg ggataaaagc attcgggtca attgtgtgtt gggttgtgaa tcttccattt
    22321 tggaattatc ctaaaacttt cttctgcgaa tgttcgcatc aatgacggtt attcctgcgg
    22381 cgctgttata tgtatgtgtt gcttgaattg ttccctttga cggtggtgtg aatgcatgtc
    22441 tggttgtttc agcatattct gtttcgttga gggtgatgat ctcgttgaag ttgattgcgt
    22501 acccgtatcg gggagaacaa ttttgcgatg gtcggatcaa ctgtcggtta tgtgaaaaga
    22561 tgcggcctgc cggtcgggca gaatgtatat ccttcacgat cgggtttttg ggatgggcgg
    22621 tccaattttc agagagggga ttgtcggaat aataaagatg cagggtgtcc caacttgagc
    22681 cgccctcttc atggatgttt gtgaaaagcc accatttacc atctgattcc agcagggtgg
    22741 tatccacagc acggatgttt tttatcaatg tcttttcgaa ctgccattga tcagggaatt
    22801 ggatacagcg gtaaagctcg attgcgttgt ttttccctgt ttctggaatc atgtataact
    22861 ggccgctgta ttcaaacagg aatggatagg aaagatgata aggcctttcc aatacgacct
    22921 gatttgacac aatattcaac gccttatcca attgaagaca ggcgattctt ccacggtggt
    22981 ttgaaaaaag cagttcctca atgaagagat agtgaatgcc attttgaacc catacaaaag
    23041 gatctgccca aaagcggtct ttaggtggcg taagggattt tagcttttcc catgaaaggg
    23101 atgtgtactc tgtttcgggc gcaaccagaa tctcccactg gtccagaaca aaaaatgaac
    23161 gcaccttttt ctgtattcga tggaaaaggc gcgtcataag ggatttgttc atggttttgt
    23221 acggattgaa ataaggcgcc ccaggcagga gtcgaaccca tatcatccct cctgcggggg
    23281 caactacgga ttgaataagg cgccccaggc aggagtcgaa cccacaacct actgatccga
    23341 agtcaggcgc tctatccatt gagctactga ggcgtaccga aattatacac gatttgcccc
    23401 gtctgcctca tggatggggc aaattggcgg tgtgcaggcg tccaatggcc tgttttacag
    23461 gggctataca aacaatactg gggtaggttt gccaacatga caagccgcat gatagaatct
    23521 tttcatctcg tctggaaagg aaattttatt catggcagac attaaggctt ttgcagagca
    23581 ggtgatcggc aatctggaaa aggtcattat tggcaagagg cagtcggttg agttgatcgt
    23641 aattgggtta ctttgtcagg ggcacgtgct gatcgaagat gtgcccggtg tggggaagac
    23701 gatgatggcg cgcggtctgg cccgctcact tgattgcatt ttcaaccgca tccaattcac
    23761 ccctgatatg ctgccaagcg atgtgactgg ggtttccatt tacaaccagc aaaagaataa
    23821 atttgaattt agaccaggtc ccattattgg gcagatcatc cttgcagatg agatcaaccg
    23881 cgccacgccg aaaacgcagg cagcgttgct tgaggcaatg gaagaacatc aggttactgt
    23941 ggatggggtg acgcacagtt tgcctgagcc gttcatggtg ttggctaccc aaaacccgat
    24001 cgagtacgaa ggcacttttc cgcttcccga agcccaatta gaccgttttc tcctgcgtct
    24061 gaggctgggc tatccttcaa tgactgatga acttcgtgtg ttgagcgacc aacagattca
    24121 acatccgttg gaaacattga aggcggttgt caaaacaaaa gaaattttac aagccattga
    24181 cgatgtgaag cacatttaca tttcacaggc tgtaaagaat tacatcgtag agttgaccac
    24241 ccgcacccgc caaagcaccg atgtgtatct tggcgccagt ccgcgcggaa gccttgccct
    24301 tgcccgggcc gggcgcgctc gcgccgccat gcagggacga gattacgtcc ttccagatga
    24361 cattcaggcg ctggctgtgc ctgttctggg tcaccgcatc attgtcagcc ccgcggctcg
    24421 tttgcgtgac ctgagctccg accgaattgt gcaggaagtc gtgcaggcca cacccgtacc
    24481 tggcggagaa ttttcacccg aaatcacatc tacacggaag aaaaccaaat gagatctggg
    24541 cgaattctcc tcgccctttt attgctgatc ggcattgcag gggtcctgac cactggagcc
    24601 gcaatttatt cgcgtttcat ctatttgagt ttattgcttg gcgttggaag ttgggtctgg
    24661 acccgctggt ctgccagtgg catggggttg cagcggtttt cccgtttgca acgtgccaat
    24721 gtgggggata tcttcgaaga gcattttgaa gtggccaaca acagccgtgt catcgccccg
    24781 tggatcgaag tctacaacca gtcgccaatc ccatttgttt caggatcacg cctgttcact
    24841 tttgttcttg gccgccaaaa gcggacacac ctcgcccgca cctggcttac gcgtcgcggc
    24901 ggttttcctc ttggccccac acgcatctcc acaggtgatc cgtttggctt gtttcgggtc
    24961 agcaaggaaa tcccagcgtc tcaaacgctt gttgttctcc ccatgatttt tgagatcaaa
    25021 tcgttcctgt tcccgcctgg cttgttgccc ggagggcagg tcatccgccg caaatcacca
    25081 gatgtcacac cgcacgcggc tggcgtgcgt gaatatgtgc atggcgatgc gatgaaacgc
    25141 attcattggc ccaccagtat tcgtaggaac cacttgatgg taaaagagtt cgaacaagac
    25201 ccgcaagccg aggtctggtt gttccttgac ctgcacagag atgtccatct cgaaaaggcc
    25261 tatcaattcg atgaaatctc tgtcgaagcg atgatgtttg gcaaacgccc caggtttcaa
    25321 ctgccgcctt ccacacttga gtatgccatc accatcactg cttcactcac gcattacttc
    25381 atcgaacgtc atcgcgcggt tggatacgcc agcgcaggaa acacctttac cgttcatccc
    25441 gccgaccgca gtgaacgtca ggaaggcaag gtgctggaga cactggcctt tgtagaagcc
    25501 aatggcaagc tgtcactggc ggcactggtc tctgcccagt cagcgcaaat gccgcagggg
    25561 tcaagcgcga tccttgtcac accatccaca cgccctgacc tattgctggc tgtggatgac
    25621 cttcaacggc ggtatcttcg cccggttgtg atccttcttg atggagaaac atttggcggc
    25681 aggcgtggaa gcactgctgg ccttgtacgc gcattgcgcg aaagacgtgt ccccgtttgc
    25741 gttgtggctt gtgacgccaa cctttccgag accctctcgg aattttcaac cgaattcatt
    25801 tcacaggata tccgtacatg gcaaacaccc gtattatcac aatagatttg aactttcaaa
    25861 ataagacgca agccattgcg tcttatttaa ttagacatgg cgacgcgaac attttgatcg
    25921 aaagcggacc aggctcgact ctttccgcgc tgaaagctgg tctggcggaa gaaggtttat
    25981 ctccccgcaa catcacccat gttcttctca ctcacattca tttggatcac gccggggctg
    26041 cggggtggct ttcacaacaa ggtgcgcagg tctttgtcca ccccaatggg gcggcgcaca
    26101 tgcttaaccc tgaaaaattg atcgccagtg ccacccgtat ttatggagat aggatggaaa
    26161 cactttgggg tgagtttctg cctgttgagc agaatcaact cacggtcccg aaggatgcgg
    26221 aagagatcgc catcggagcc ctgcgttttc ttcccatcca caccccaggc catgcggaac
    26281 atcattattc ctatttattt gaagacctgt gttttagcgg tgatgtaggc ggggtgcgga
    26341 ttccgggctt cccttatttg cgcgcaccga tgccgccgcc tgaattacat tttgcaaaat
    26401 ggcgcgaaag catttcgcat ttgaagtctt tgaaatttgc ccgcattgcg ccaactcatt
    26461 ttgggatctt cgatgatgtg aactggcact taaatgaact gcaaaaaaat cttgatgaag
    26521 ccgagaaatg gcttgaagag atcatgcccg ccgacccgcc cgtggacgag ctgcgcgaaa
    26581 aatttacagt ttggatgaat gcccaggccg cctcgctgaa cttaagtgag gatgttctgc
    26641 gtgcctacac attggcgaac cctctcggca tgggtgcaga tgggttgatg cgatattgga
    26701 agaaggtgcg gagctcgccc atggcatgac gttaatatgt aaactatggc gaaatgtcac
    26761 gcgcaaagta tgatgcttga aacgtggcag aatcccacta taaagatata ctttcacaac
    26821 aaggagtaaa tatgaaggac tttctcgcga tttcggatta ttcatctgaa gaaattcagg
    26881 atatgcttga tctggcggca aaactcaaga agcagtattt caaaaaagga aataaaccca
    26941 ttttcaaagg caaggtgctg ggaatgattt tccagaaacc gagcctgcgc acgcgcgttt
    27001 cttttgatat ggccatgcgt cactgcggcg gcgatgcgct gtatctttca ccgagtgaga
    27061 tcggccttgg caagcgcgaa tccattgcag atgtggcgcg ggtgctctcc gggtatgtgc
    27121 aggggcttat ggcgcgggtg ttcgatcacg agcatgtgct tgagcttgca aagtggtctg
    27181 atattccagt ggtgaacggt ttgagcgatt acaaccatcc ctgtcagggc atggctgatg
    27241 cgctgaccat tcaagagaaa tttggaaaga aatccagggg tctgaatgta tcgtatgtgg
    27301 gtgatggcaa caatgtgacg gtttcgctca tgcatgtctc agccctgctc ggttggaatg
    27361 tgaccgtggc cacccccgaa ggatacgatg ttaacccgaa ggctattgaa atcgcccaga
    27421 agatcgcgaa gaagaccaag agcaagctga cctttgtccg cgacccgcat gaagctgtga
    27481 agggcgcgca cgtcatttac accgatacat ggacatccat gggacaggaa gaagaaacag
    27541 ccaagcgcga gaaaatcttc ccgccctatc aggtgaatgc aaaattggtc ggcgaagcca
    27601 gcaaggatgt gatcgtgatg cactgcctgc cagcacaccg caaccatgaa ttgaccgacg
    27661 atgtggctga cggcaaacat tcggttattt tcccgcaggc gcataaccgc ctgcatgcgc
    27721 aaaaggcgat cctggcgcgt ttgtttgatg tagcgtagga attatggatt gggatttttt
    27781 acttcgatct ccaactccca acctttaact ttcaatcttc aattatcaaa tagaggaaac
    27841 taattttatg aacactcaag actttatcaa aatggaagaa cagtacggcg cgcacaatta
    27901 tcacccgctc gatgtggtca tcgagaaggc ggaaggtgtg tgggtttacg atgtggatgg
    27961 cagaaaatat ctcgattgtt taagtgcata ctcggcggtt aatcagggcc acgtccaccc
    28021 tgaaattttg aaggctttgc tcgatcaggc caaacgagtt tcgttgacct cgcgtgcttt
    28081 tcgtaatgac cagttgcccc tgctttacaa agaactctct gaaatgacgg gttacgaaat
    28141 gtcgctgccg atgaattcag gcgctgaggc ggttgaaacg gctgtaaaac ttgcccgcaa
    28201 atgggcttat caagtgaaaa aggtgccccg ccatcaggcg gagatcatcg tggcttcggg
    28261 taatttccac gggcgcacag tgaccattgt ttcattctcc accgagcctt cctatcgcga
    28321 tgattttggc cccttcaccc cgggctttgt caccgttact tatggcgatg cggatgcgat
    28381 tgaaaaggcc atcaccccaa atacagcggc tgtgatgctt gaaccgatcc agggcgaagc
    28441 aggggtcatt attccgcctg ctggatattt gaagaaggtt gcggaaatct gtaaaaagaa
    28501 caacgttctc tttgttgcag atgaaattca gacgggtctt gcccgcactg gaaaactgtt
    28561 cgcttctcac catgaagatg tccgccccga tattgtcatc atcggcaagg cgcttgcagg
    28621 cggcttttat cctgtgtctg cggtgctggc tgataaaccc atccttgggt tgttccagcc
    28681 cggtgaacat ggttccacat ttggcggcaa tccattggcg gcggcggttt cccgcgcagc
    28741 cttgcgtgtg atccgcgaag agaaactggc tgaacggtct cagcagttgg gcgagtattt
    28801 tgtggaacag ttgagtgaga tctccagtcc gcacatcaaa gaagtgcgcg gacggggctt
    28861 gttgatcggt gttgaactca agcctgaggc aaaaggcgca cgtcgttttt gtgaagccct
    28921 gcaaacgaag ggtattcttg caaaagaaac ccacgataac gtcatccgct ttgctccacc
    28981 gcttgtgatc gacaaagaaa ccatagattg ggcgcttccc tccattcgtg aagtgctaaa
    29041 tatgaagtaa tcttgaaacc ctaaaggtga taaaaaacct ttagggtttt ttacggaggc
    29101 atacatgtat atcggaattc caaaagaaag cagaaccttt gagtatcgtg tgggtctctc
    29161 cccagccggg gttgagattc tgacacagaa cgggcaccag gcttacgttg agcatgaagc
    29221 gggcgtaatg gctggcttca gcgatgcgga ttatgaaaaa gctggtgcgc gtctggtttt
    29281 ctcgaaggag gaagtctttg gccgtgcgga tctacttttg aaggtgagcc gccccatgca
    29341 agaggagttg aattggctgc ggcctgagtc gatcctggca ggttttttgc acctcgcctc
    29401 aacccagcag gacaagatcg atattttgct tgaaaagaag atcacgtctg tagcctatga
    29461 acaaattaag gcacctgatg ggtctctgcc tgtcctgcgt ccattcagcc agatcggcgg
    29521 cgcaatggca gcgcagattg ccgctcgcct gttgcagaac aattggggcg gcaagggcat
    29581 tttgatgggc ggtgtgccag gcgtgccgcc agccgaagtt gtcatcctcg gggcgggtgt
    29641 ggtggggaca tctgccgcgc aaatgttcct cggcatgggt gcgcatgtaa ccgtgatcga
    29701 tcaaaacctt gctgctcttc aacgcatctg ggatcgattc ccgaatattg ccacaaagat
    29761 gtctacaaag cgcaatattg aacgctcggt catgtatgcc gatgtggtgg tgggggctgt
    29821 gctgtcacct ggtcagcgtg cgccgatttt ggtcacgcgt gaaatggtgc gctccatgaa
    29881 accacgctcc gtcattatgg atgtcagcat tgacgagggc ggctgtgtgg aaacatcgcg
    29941 cccgaccaca cacgagcatc ctgtgtacat cgaagaaggc attttgcact attgtgtgcc
    30001 gaacatgcca ggtgcggtgg ctcgtacggc cacgcatgct tttgtaaatt ccgccatacc
    30061 ctatatcact gaaattgcgg cacttggaac gaaagagatt ttgagcaatc catcccttga
    30121 acctgcgatc aacacgcacg acggcaaact gctcaacctt gtgcgtttga gcgcgcagca
    30181 gcaggaggat gaataatatg actacagcca gtatttatca atcacgagtg acgacggcac
    30241 aggaagccgt caaaaccatc aaatcgggga atcgcgtctt tctcaccggt aacgtgtctg
    30301 tgccgcaaaa gattttggcg gcgctggtgg agtacgcccc caacctcacc gatgtggaga
    30361 tctgccaggc attgaccgtc ggttccgccg attacgtcgg ccctgaaatg gaaggccatt
    30421 tgcgcgtgaa ttcgatgttc atcagcgcaa atattcgtaa agcagttcag gaaggccgcg
    30481 ccgattttac gccagtcctg ctttctgaat ttccgctttt gttcaaacgc ggcgtgttgc
    30541 cattggacgt tgcggttatc cacgtatcca cccccgatga acatggattc tgcagcctgg
    30601 gtgtggaagt tgggcttacc aaatcagcag ctgaatcggc gaagatcatc attgccgaag
    30661 tcaacgaaca gatgccgcgt acattgggag atgcgttcat tcacgtcagc cgattgactc
    30721 atatcgtgcc tgtcaattat cccatccccg aacatcgcat gaccgccgaa ggtgacatgg
    30781 atatcgtgca gaagattgct ggccatgttg cttccttaat tcccgatggc gccaccatgc
    30841 agcttggcat tggctcgatc cctgatgctg ttttgaagtt cctttttgat aagaaagacc
    30901 ttggcatcca caccgaattg ttttcagatg gcgtcattga ccttgtgaat gcaggtgttt
    30961 tgaccaatgc ccgcaaatcc cttcacccgg gcaaggtggt ggcgggattt atccttggca
    31021 ccaaacggct gtacgaatgg gtcaacgata acccgatggt ggaaatgcac cccacggaat
    31081 atgtgaatga ccctttcatc atcgcccaaa acgaccgcat ggtggcggtc aactctgcca
    31141 ttgaagtgga cctgaccggg cagatctgcg ccgatagcat tgggccgaaa ctctacagtg
    31201 gtgtaggcgg ccagttggat tttgtttatg gtgcttcccg ctcaaagggc ggggtgccga
    31261 tcattgccct tccaagtgtt gccaccctgc gtgatggcac acagttgagc aagatcaccg
    31321 ccatgctcaa gactggcgcg ggtgtggtca ccagccgcaa ccatgtccgc tttgtggtga
    31381 ccgaacacgg cattgccgac ctgtatggca agaccattcg tcagcgcgca caggccctca
    31441 tcaatgtggc acatccgcaa ttccgcgagg aattgacccg tcaggccaaa gaactgcatt
    31501 acgggtaaaa gcttttctcc atgaaggaag atcatgccgc agggatattc actcccctgc
    31561 ggcatttttt acgccttgac atcatcggct tttttaccta tcatagcgcc cgtcaactgc
    31621 atcaatgagg gaagacagcg tatctttttg ccgttttggg ggtgtattat tcaattgcca
    31681 tccgcacatg gagcaggttg aactttatta catttataga aggaagcaag tgaacgttat
    31741 gagaagagca aagatcgttg ccaccatcgg ccctgcctct gattcagagc aggctctcga
    31801 agccttgatg aaagcgggta tgaatattgc ccgtcttaat ttttcccacg gaacgcacga
    31861 acagcatcaa aaacgcgtcg ccgccattcg tgcggtttcc aaacgacttg gaatgcccat
    31921 cggtattctg caggatttgc agggacccaa gatccgtgtt ggcaaacttg ccgctccgct
    31981 gcagctcgca gtggatgaac acgtctgtct ttacgccacc aacgacgaac ccccgcaaac
    32041 tgaaaaccag cttctcccag ttgatttccg cgaactcttt gactccgtcc atgagggtga
    32101 ccgccttctc cttgacgatg gccgccttgc tttgcaggta atctccgccg aaggacgtgt
    32161 ggtgcatgcc agggttttgg tggggggcac attgtcctcg cacaagggca ttaacctgcc
    32221 tggggtcaaa cttcgtattg caggctttac cgaaaaagac aaaaccgatc ttgcctttgg
    32281 catctcacag ggtgtcgatg ctgttgccat ttctttcgtc cgcagtgccg acgatgttcg
    32341 ctcggtgcgt gctgccatca aggaattttc gcagggcagg tttgtcaccc cattgctgat
    32401 cgcaaaactt gaaaagcccg aagccctcaa cgaattggaa gagatccttg atgtggtgga
    32461 tggtgtgatg gttgcccgcg gcgacctcgg cgtggaactt ccacccgaac gcgtgccttc
    32521 tttgcaaaag cacatcattc gctctgcaaa cgcccgcgcc aaactggtca tcactgctac
    32581 gcagatgctc gaatccatga tccagaatcc gctgcccaca cgcgctgaag cctctgatgt
    32641 agccaacgcc atttttgatg gcactgatgc ggtcatgctt tctgctgaaa ccgcctcggg
    32701 tgaataccca agtgaggctg tttccatgat gtcgcgcatc gtcagcgaag ccgagtcgca
    32761 ttttacagag tggggcagtc gtcaggatgg cagggcaggc ctcggtgata gtgatgcggc
    32821 ttccatggcc cgcgcagcca aagcacttgc caccgacccc gaagtcagcg cggtttccgt
    32881 cttcaccatg caggggcgct ctgcctggct tatgtcaaag ggtcgccctt cgaaaaatat
    32941 tctcgcattt acacccgagt acgaaacctt caaccagctt tcctttttgt ggggcgtaca
    33001 gccgaacctc acaaaattcg caaacagcat ggatgaaatg ctcgccaatg tcgatgccgt
    33061 gctgctcaac tctggcattc gctcaggtca gcaggtggtg ttggtctgcg ggtatcccgt
    33121 cggtgcgcag cgcccgccta acatggcact gcttcacacc gtcggcagcg atgcgtctgt
    33181 caatctcgcg cgcaaacttg ccgaaagcaa cgttgtgaag cgatagctct ttttctttgg
    33241 gaattaacaa agcggatgca gtttttcaaa actgcatccg cttcttttct taaccgaaac
    33301 ttgatcctgc tgaaattgca tcccctcccc gaaaaacgtt cttactcccg tgttgcgatg
    33361 agaatgccca cactgatcaa agctccgccg aacaggaaga gcggggtgat tttttcggcg
    33421 agaaagaacc atccgagcag cgtacccact accggctggg caaagaacgt cagcgatgcc
    33481 acagcggcgg gtaattctgc gaaggcgtaa ttccacagga acatggcaat tgccgttgag
    33541 acgatcccca aaaataacaa gccgccgata atgccaggtg taattgcacc aacaccctgt
    33601 gtgttgactt cccacaatcc gaaggcgatg cttgaaggca gaccgcccaa caacatgacc
    33661 gtgctggatg taagcagatc acccgattga ctcaccttgc gcaccaacac cgaatacaac
    33721 gcccaagtca gggccgccgc aagcagactc atattgcccc agaacagggt gggggaaagt
    33781 tcggcattgc gcggatctat gaccgccaac acccccagcg tggatacgac cagggcaaca
    33841 atgcggcgtg tggtcgtttt ttcgccgagc aaaaatggtg caaagatcag aacaaaagcg
    33901 ggtgttgccg atgtcaccaa cgatccattg gatgcggtgg aaagtttcgt gcccacaaat
    33961 tgaaaaccga gtgaaacccc gtaccctacc acgccgacaa aaaaactttt ccagaattgt
    34021 tcttttgaaa aaacgacctt gtctttgcga aaatagatga tgatgcccaa taccagcgcc
    34081 cccatgacaa gacgaatgct caacaatgca aacggaggga tgacctccaa aacaaccttg
    34141 ctgacgacat acatcccgcc ccaaatggac gcggccccca accctgcaaa taaccctgcg
    34201 acactgtgtt tatgcatagt ccccgattat atcgaaaagc ggttcatcgg gctcgaattg
    34261 ggcttatggt tattctgata cagcagggat caatctctct gacgcccaca ttttatatgc
    34321 cgcccgtgca tggatttcat gcgaggcatg ccagtttttc atatcatcca ctgcccagta
    34381 ttgcagggca tgaccttcat gtgaaatctt caactcgccg ccgatgattt cgcacaaatg
    34441 aacgaccgac accacagtat gcggccccat tttggcgctt ggttttcggg tgaaaacgcc
    34501 cacaagttgt ttgatctcaa cctccaaccc tgtttcctca cgtacttccc gtagaattcc
    34561 atcggctggt gattcgttcg gttcaacaaa tccagcaggc aggcaccaac cgctgccatc
    34621 caccctttcc atgaggagga tctcgccgcg ttcgttgaag atcgctgcat tggtccctaa
    34681 ttttggcgtg atggtgccaa gttcatttga aaatccctgc tttatcttct ctgagggaat
    34741 atttagaata tcgctgtatg tctttgcggc catttccagc aggcgttcgt aacgttcctt
    34801 atcgtattcg ttggagacgt gcagcaggcc gttgcgtgca atggtttgga cggtgtccaa
    34861 aagggagaat acatccatat tcactgaaat gaactcctgt ctatttgctc atcaatgtca
    34921 aaaacgaatt cacgaccttc gggtcaaaat atttccctga ataggtttcc atatactcaa
    34981 ccaccctttg ttgcttccag gcgcggcgat atggccgatt ggaaagcaag gcatcccaaa
    35041 catccaccac cgagaaaatg cgggcggcca atggaatttc ctcacctttc aaggcacggg
    35101 ggtatccact gccgtcccag cgttcatggt gggcatacgg aatttcaagc gcagggcgta
    35161 aataggcgat cggttggagc atttcatagg catagaccgg atgcatacgc atgatcttcc
    35221 attccttgat cgtcaggcgc ccgggcttga gcagaatttg gtctggtacg cccattttgc
    35281 cgatgtcgtg cagtagagcc ccgcgatgca catggaccag ttcatctgcc tgcaagccca
    35341 tggaactggc cagcagcatg gtcatttcgg tgacgcgttg agtatgccct tcagtttccc
    35401 gatcgcgcag atccaatgcc cgtgaccagc cctcgatggt ggtatcgtag gcaaacttca
    35461 aatcctgatt cgagcgctgc aggttgttga ataactcggc attgtcaatt gccagtgccg
    35521 cctgccctgc cagtgtatcc atcagttcca gccattcttt gtcagggtgg aagggctcac
    35581 ggtggaaaac ttcaagaaca cctttcactt ctccctttgc gatcagcggg ttggcatgat
    35641 aagcgcggat attttccaaa cgggcaaagt ctgaatttga aaaaaactga tccgagttgg
    35701 aaagattggt aatgctgacc tgtcttcttt ccagcgcggc ttgtccggcg tgtttatcac
    35761 ccagccgcaa gcggacctgc ctgatcattc cttcattttt gaaaccatgc cctgcaaagt
    35821 actccagcat ttgagtggct ggccgcaacc gcaaaatgct gaccgcatcc gcctgcaatt
    35881 gcgtcagggc attgttgatc aacatgttga tggaaacaga caggtcaaag ttggaagcga
    35941 tcgtctggtc gatctgccgc aggatggaga tgtgagtcag gtttcgtttg gtctgctcat
    36001 gcaaacgggt gcgatgaatc gcatttcctg cgatctcagc cagcgttgtc agcaggcgca
    36061 cttcttcaga agataactct cgcggcattt gcaccgaaac aaacaacaca ccgaccactt
    36121 cctgcgaagt ttgaatggga atacagactg catcccatcc atttggaatt tccttccact
    36181 cctgcatgga ttcttttaga tgatatacct cgccgctgga ataaacgaat cctgcgatac
    36241 cttcagcggg tctctgcgaa tgcggcattt tctccccgaa ccatccccgt tgaatgtaag
    36301 attgcagggt tccgcttgca gggtcataca gatagatact gcctgcattg gtgtccaatg
    36361 ctttcaaggt ctcatccagc aacccgggta tcatctcttc cagagaattt gcggcgcgca
    36421 atgtgcctga aacacgggca atggtttcaa gttcatccag cctgcggatg gtgttttcca
    36481 agagacgggc gttgtaaatt gccgccgccg cctggcttgc aaacagttcc agcaggcggg
    36541 catcggcatc tgtgtatttg cggtgggaac ttcccaattc accaatggtc aacacgccga
    36601 tcatttcccc gccatagacc attggcacgc ccaacacggc cttcacattg agaccgtcaa
    36661 acaaggggga gcgattttcc caggttgagt actcgttgac aatgatcgtt ttgcgagtct
    36721 gtaccaccag cccgctgaca ccctgaccgg gtttcaaccg cactggtccc gctgggaaca
    36781 tctctccgat tctgacggcc agatcaagtt gttcacgggt ggcatcgtac aggtaaaagc
    36841 ccgccgaatt cgaatcaagc atcgattcgg cacgcgcagc gatcacttgc aaaacagacc
    36901 gtagatctgc ctggcttttc gcgaggctgt gtgcagtatc gtacagcaga gcaaattcat
    36961 cggcccgcct tcggacctgt tcttccgcca gtttttgttc ggtgatatcg aacatgaaac
    37021 ccaaagaaag aaatggattc ccattttcat ccgatgcaac ggtcgccaga tcgcggaccc
    37081 atatcatgcg gccatccctg gtatacaggc gatattccaa tttaacaggc tccccagtga
    37141 taatggaccg ggtttccaca tcggtcacca actggcgatc gccagggtga aggctgcgct
    37201 gccagatttg ctggtcagcg atccactcct cggcggtgta tcctgtcagg tctttgatct
    37261 gcgggctgac gaatttccaa cgcatatctg gtcccagccc ggcgctatat accacggcag
    37321 ggatctgttc catgatctgt cgatatcgga attcagcggt ttggatggct tgttccgcca
    37381 atttgcgctc tgtaatatca gaaaagacca cgaatacccg tttccaggtc tgttcagcgc
    37441 cgtaggcgag ccttagccac atcgtaacag ttcgtatttc accccgcatg tttaatgctt
    37501 caaattcggt gtcgtaggat acttttccat ttatcaaggc gataaactgc tctttgaata
    37561 ccctgaaact cttgctgtcg atccgtatcg gcgtggtcga tttatcgatc agttttgatt
    37621 tgtcctccac gccgtaaaac tgaagcgccg cctgatttac attcaaaatt ttgatgcggc
    37681 tcacacattc attcactgcc tcaggatggg cgtcgaaata tgcggcaaag tctgtcacgc
    37741 ctgcatcgga caactcattg agatattcac gggttgaggt gaagtctgat tcccagatac
    37801 ccacgggtac attttcaaac aactcgtaaa aatgctgttg actggcgcgc aattcctctt
    37861 cagcgcgttt ccgttcaacg gccagagcca cctgtcccgc agttgaggtg aagaactcct
    37921 cctccgcctt cgaatagcaa aattcctttt cgtaatcctg cacaaccatc acgccgatgg
    37981 attcaccgct gatcttaagc ggtgcgccca gccacgagag ggaacgactg ccgatcaact
    38041 ccatttcacc ttctgctatt agctgctgga atctttcctc ggttaacagc aacgacttgc
    38101 ccgttcgaat cacatatgcg cttgcgcttt tttgcatttc cgattgcgga gccagagccg
    38161 cattgatctg gtccaccgaa tacgctcctt cgtacatccc agttgttttg ttcttaagga
    38221 tgacaaagta gttttcagcg ctcaatgctt tctttagcgc tgcatggacc tttgcaaggt
    38281 attcctgcag gttattcgtt tccactgcgg cctgcatgat ttccatcaag gattggcgct
    38341 gggcttctgc ctgcttttgt aagctcatat ccatggctga aaccaggatg gatatctgcc
    38401 cattattcca aattggatta agcccaacct gtatggggaa tgtactccca tccctgcgaa
    38461 ttgcggtcaa ttcacgccct tccatctggc gggggtgggg atgatttaaa taatcactac
    38521 gatgaaaggt gtgatgatgg cgtatgggtt caggcaccaa ctgctcaatt cctattccct
    38581 gtaagccgcc cgcctcataa ccaaaaatat tctccgcttg tttgttggca agtgatattt
    38641 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcccgcct cataaccaaa
    38761 aatattctcc gcttgtttgt tggcaagtga tattttccca ttcgaatcag cgatcaacat
    38821 gccatgcggc acagcttcca tcgcgctgag aaaaccctcc caatctggat tcttgtcttt
    38881 tttttgaatg atctcccccg catacccact tgtaaaacct gccacaactg cgaggaaaaa
    38941 gtcaatttca atcgcccatc gaagcgacat tgcccgatca aattgtccac ggtttgccag
    39001 ggcaagcaaa aggaggttga tgggaaaact gaatacccca acccacaccc cgatcttctt
    39061 cccaaaaatc cacccggcga cagccaccgg tagaacgaca aagatggtca ccccttcccc
    39121 gtatcgcgga agaaaaacag ccaatgccag ataataaaaa accccaaagg tgaatacaat
    39181 ccccatgcca acccagtgga ataagggtgt attcttgttt ggaggtttca agaaatggct
    39241 ggttttattc attaagcaca aaaacgcctt tgaagatcac aatgcgtacg aatggacgat
    39301 ttatggtgaa tcgcgaccgg taaatttgag tttggagatg gaatctgaac gggcttaccg
    39361 gtattataca ttacgctcat tatttggaat atctcccccg aaaaaacgaa tcctccctaa
    39421 ctgctggctg gtcagccaac aaacggcatt tccctgctga caagatcccc cgctgactat
    39481 ggcttgctgc gataccaata ttgatattgt tccttgaacc caagcgagga atacaaatgc
    39541 agtgcgaccg tattgtggag ttcaacctgt aagtacgcct gtttggcctg attttgttgt
    39601 ccccacgcca gaagcccttc caccacctgt cttccatatc ctcggtttcg gaaggcgggg
    39661 tcggtgacga tatcgaataa gccgagaaat tctgactgta agacccccat gccgcaggct
    39721 acaatcctgt cgtcaaactt aagtgataaa aaacaatgac gcggcacaac atttaacagg
    39781 atctgtcgca gcgtttcccg attttcttca gcgaccccat tcatatggca tacggcattc
    39841 agccaagcgc tcgaaaggct ttcctcaagc tcagtttgta acggaaggtg aaaattggaa
    39901 aaatccaatt tcagggtctg cacgctggtg gatgatggag atttttgaaa tcctctggct
    39961 gaaagttctt catccagcat ttcagggtag acatgtggag tgagtttgaa aactacaggg
    40021 agttgtctgc tcttatagat ttcctcacag aatcgaatct tctcgtcaag cggaatgctt
    40081 gaagcatgga tcgggttgat ggaattggcg cgtttggtat atccgttggc aaaccgcagc
    40141 acccaaccat cataatggac ggtttgaagt gcggcccagg catttaacga gaattcctca
    40201 agagctgtga tcatgctttc tacttgcccc cgtgctgcac aaggactttt gccgccgctg
    40261 ttgctttatg gaaggtggtg tgatgcaggg gagttttttc gttcatatcc ttcgcattta
    40321 catctgcacc tgcttcgatc agcatttgaa cgagaaccgc ctgatttgca tgtgcagcag
    40381 catggagggg agtggtgccc cagtggtcat tgctgttctg tgcgtgaaca tccgccccaa
    40441 ctttgagcag aaatgcgaca acctcacgat gccccttcca ggtcgcgcaa tgcaatgctg
    40501 tggaaccatc tgtatctctg gcattgagca gagacttgtc agcctttagc aatgctttga
    40561 gggcatcgag atccgctttc tttgctgctt tgatgaagtc ttttgccatg tcagccattt
    40621 gtgtttctcc ttgaaaagtt tttatttata gaggtacttc gaaaatttgg gttgtcaggg
    40681 tgtcgttttg aattgtaacc aatcgataat gaggcggtaa aaggcaggca agcggctcat
    40741 cctgtagaac gaattgaggc gctgtggagc gcagcccaaa aatgggaact tcgttcacga
    40801 tactttcata cgtagcatgg acatgcccgc aaaagatacc gagtacaggt ttctgagcga
    40861 tgatctccca gaaacgggag gtatcgtctg caataaaatc gtccagccat ttgcttccaa
    40921 gttttaccaa ctggtgatgc atcatgataa tggatggtat atcctttttt attgtttggg
    40981 cgaggaattc cagcatttca ggatgggctg ttgcctttga ttctggtccc cagtccaaac
    41041 agataaattg gatgcctttg tgtatgaaag ctgcattcat gatggatgct gattggcttt
    41101 ggggaaacaa atacttgtaa aaattcttgc ggtcatcatg attgcctggc agcacaaaca
    41161 tgggcaaatt ttgcagcccc tccactgaaa tgtggagagg ccctggaatt tctgccaggt
    41221 tattttgtac accgagtagc tcaagggagg cttgataaga ggcttccgaa gggatatcca
    41281 caagatcccc ggtggaaaca ataaaatcaa cgtgagtccc tgcgttgtga gcaatatgcc
    41341 gcagaactgt gcgtaaagca tatctggttg agaaacctcg cagaaattca ctgtcggagt
    41401 cggtcaggtg gtgatctgtg atttggatga aggaaaagtc cacagctctc tctttttaga
    41461 atatttttta gcggattgtg ctacaaattt ctcagctcaa gcaaatccca tttgtttcca
    41521 tagagatcaa agaacactgc tacgattccg taaggctcgt ggcgagggtc ttcagcgaac
    41581 cgcacaccat gcgactgcat gtgttgatgg tcgcgctgaa agtcgtccgt gtggaggaag
    41641 aaagccaccc tgccgcctgt ttggttgcca atgtgctggg tctgttcggc ggtgtctgct
    41701 ttggccaaaa gtaaggcaga tccatttcca ccttgtggag agaccctcac ccaacgtttg
    41761 ttgtcgtcaa ggggtgtgtc ttcaagcagg atgaagttta atttttcggt gaagaacgca
    41821 atggcttcat cgtaatcacg tacaaggtaa gtgacatggg cgatggacgg cgacatggcg
    41881 gctcagtcgt aggctttgtc gcgtcgaatt tttttgatgc ttccccgatt ctttttttct
    41941 ttcaaccttt cctcttttga ggcttttgtt ggttttgttt tgtgacgcac cttcggtttt
    42001 tcgtttgctt ttgccactaa atcatagaat cgctgaaggg cgtcttcgcg gttttgctcc
    42061 tgcgtgcgat atttcttcgc ctcgatgatg agcactccct cagcggtgac tcgactccca
    42121 gaaagccgaa tcaacctccc ctttacgtca gaagcgaggg agggggagtt ggtaatgtca
    42181 aagcgcagtt ggaccgccgt cgcgaccttg tttacattct gtccgcctgg gcctgatgcg
    42241 cgaatatatt ccagtgagat ttccgattcc agatttttca tttccccgaa ttatatcgta
    42301 gggacacagc ggcgccgctg tgtccctaca taattatgct tttactaccc gtggggcgag
    42361 cagccacatg atcgcaagga taagtccgcc tgcgaaaaat gggacagggc cgccaaacca
    42421 ttggaacagg gctccataaa aaagcggcgc aatggcgttt gcggcgctat acgctgcgga
    42481 ggatgcgccg agcattccgc caacttcgtt tttatctgag gcttttgtaa taaggctgtt
    42541 cactgcgggc tgcagaacac caccacccaa agcggcaggg aaacttgcaa gcaggagcca
    42601 aatgataccg agccagcctt ttgctgcttc atcagggagt tgaatgttga tgctttgaat
    42661 tgaaacgcgg aactcaccct gccccgccat gctttcaagc accttcgctt tttcatacca
    42721 gggaattggc actgcgggtg tgagagccgt tccgatcaaa ccaatggcaa gtgtgccgat
    42781 tcccatcagc accagccagc gatcaccttt ttgttttgac catctgccga tcaagccgcc
    42841 ctgtaccacg atgatgaaca aacctgccag cacgaacagg cccgaagtat cccgtgcatc
    42901 catgccgagg cgggtgagcg tgaataatga gaaaagctgt tcatacccgc caaatgcgat
    42961 ctgataaaag aacatcagaa tgagcaaaaa accgatcgaa gggcggctga gcgcttcacg
    43021 taacgcgccg aatgaaaatg ggcggcgttt gcgggaggct tcggggtttt caacttctgc
    43081 gtaggtttcc ttgaaccaga ataaggttaa caggatcgag gccagcgaaa agaaggcggc
    43141 ggtgaaagca actgcctggt agttttgacc tgtggcggca agtacgataa atgcaaggat
    43201 ggggccgagt acaaagccga tgccaaatgc cgcacccacg agccccagcc cctgtgtgcg
    43261 ggtcttttcg gtggtgctat cggcaatggc tgcctgcgct gttgaaatgt tcgcgccaga
    43321 gaggccgtca ataatgcggg agatgaataa cagcaaaagc gagtttgcaa accccaacat
    43381 gatgaatccg atcaaagtgc cgatctggct tacgaccaga attggtttcc gtccgaagcg
    43441 gtctgagagg cgcccgagaa tgggtgctcc taaaaactgc atcatcggat aggtggcttg
    43501 caaaattccg atcaccagcg gcgaagaccc aaaccgcgcc gcgtataacg gcagcaacgg
    43561 gatgatgatg gacaggccca tcaagtccac cagcacgatc actaatacag gcagaatgcg
    43621 tttgaaatca agtttttcgt ttgttgtttc tgtggtcatg gtttctcatt ttataaaccc
    43681 tcccccaaat cctcttttgg gatttggggg agggtagggt gggaacgggg tgtgtgttag
    43741 aacatcaacg cgttgacttt gtcgatcatt gagcggtcag gtctcaattc ggcttcggta
    43801 aggcggttga gcggtgtatc cacaaggttg tacattgaag tgaggttggg cttggaggtg
    43861 tccacataca acaagcctgt gatcagccag ttgttgcggc cagcttcttc catcacacgc
    43921 agggcttcga aacggttggt ggggttgtag cccttttcga gattcttcaa gatcacggtt
    43981 gagccgtcgt gcatggctac ttcgcgcact tcgccatctt ccatctcgcg ttcgagaatg
    44041 atctcgttgc gaggggcaat gtaggagatt tcatgcaggg gttcttcgtg atccttgccc
    44101 cacgcataag agtgataggc gttttcctga ttgttgaagg tgacacacgg gctgatgata
    44161 tccagcactg caatgccatc atgagcaagg gcggccttga gcaattcttt tacctgtttg
    44221 gggttgcctg caaaagaacg ggcgataaag gtcgcatttg agaccaatgc ctccatgcag
    44281 atatccacag gcatgtaggg attttcaccc tgcttcttca ggctcaggcc tttttcagcg
    44341 gtcgcagaga actggccttt ggtaaggccg tacacgccgt tgttttcaac aatgtacaac
    44401 atattcacgt tacggcgcat cacatgtttg aactgaccca tgccaatgct ggcagagtca
    44461 ccgtcgccag aaacgccgat gcccttcaag tgatggtcac caaataacgc gcctgttgca
    44521 atggacggca tgcgtccatg cagactgttg aagccgaagg agcggttcag gaagtaggtg
    44581 ggtgacttcg aagaacagcc gatgccgctg aatttcacca cgtcttcggg tacgatgttc
    44641 atttcataca gcgcggtcac gatttggttt gagatcgagt tatgtccgca gccctggcac
    44701 agggtgctcg ggttgccgcg atattcattt ttagataagc cagcgaggtt gacatttgtg
    44761 gcaggtgtgc cggccatggg gagagcttct gtcattttat atgctccttg ataaagttat
    44821 tacttccgct tcgacttcac ggatgacttg gacttggcaa cgggtttctt tgccgctggc
    44881 tttttagtcg ctgctttttt ggaccctgaa actgcttttg ccttcgacgc agtttttgcc
    44941 gctgcggctt tcttgggcgt tccagctttc gcgtatttgg caagaattcc ttcgcggacc
    45001 catttggcag aggcaggcat gccatcgcca aaagccacag atttgaagcg gttcgcaaat
    45061 tgcggatatt ccgtcagcag aatttgatac atttggccat cgcggttcat ttccacgatg
    45121 aagatttggt cgtattttgc gaggaaggca tccacttcct tggtgaaggg gatggcacgg
    45181 acgcgcatga aatcggtctt gataccatgg tccttttcca actggtgctg ggcttccaca
    45241 actgctgctt cggtggagcc ataggcaatg atgccaaccg tggcgccttt tatggaatgc
    45301 atcactggag cagggacata tttcttcgcg gtctcatgct ttctcttcaa gcggtccatg
    45361 tttttgaggt agatatccgc ttcttcgctg tatttggcgt attcatcgtg gcctgtgccg
    45421 cgggtgaagt aggagctgag cgggtgcttg ttgccaggga aggtgcggaa accaattcca
    45481 tcaccgtctt tatcgaggta acggccccaa ttgcctttga gttcttcgat atctttttcg
    45541 aacaagacct tgccgcgatc catcggggtg gatggatatt caaaaggttt gctcatccac
    45601 tggttcatgc ccatgtcgag gtcactcaga acaaagacag gagtctggat gcgttcggca
    45661 acatcaaatg cgcgccagcc gaactcaaaa cattcaaata catcgcctgg caggagggtg
    45721 acagagtgcg aatcaccgtg accgataaaa tgggtgaagg tcaaatcgcc ttgtgaagtg
    45781 cgtgttggca ggcctgtgct ggggccaatg cgttgcacat cccagatgac cacaggggtc
    45841 tctgcatagt aggcgaggcc agcgaattcg gtcatcaagg acaggcccgg gccagaggtg
    45901 gaggtcatgg agcgtaaacc tgcccagcca gcaccgacgg tcatacccag ggcggcaagc
    45961 tcgtcttccg cctgaaccac ggcgtaggtg tgtttaccgt cttcgcgctt gcggagttgg
    46021 gggagataat cgttaagtga ttctgcgagt gaggttgcag gggtaatggg ataccatgcg
    46081 gcaaactgca caccaccaaa aatggaaccg attgcggctg cggtatttcc gtctgccatg
    46141 atcatgcctt cggtcttgtt catggtttca acgtagaatg gatccttctt ctcgaggttg
    46201 ctcctggccc attctgcacc tgctttgacc gcgccatagt tcatgtcaat gggtttctgc
    46261 ttgcccttga agtggaaggt tagcgctgca agtatcttgt ccatatcgag attgatcatt
    46321 tgtgccagaa caccgacgta aaccatattc cctacaagat cacggaaatc ggtgggtaca
    46381 ttggggtcat tctttacgat ggtttttacc ggcatggggt aaatggcgat atctgtgcgg
    46441 ttgatgggtt gcttgatatc gtcggcgtag aaaaatgctc cgccaggggt gacagatgcc
    46501 aggtcacgta cgaacgaatt gggattcatc gcaatgacaa tgtcacggcg ttcgttgtgg
    46561 gcgataaatc catccttgtt ggcgcgaatg gtgtaccagg taggcaggcc ttgaatgttg
    46621 gaagggaaga ggtttttgcc tgagacgggt atgcccatct tgaagatgga tcgcaggatg
    46681 gtgttgttgg cggtggaact gcctgagccg ttcacggtgc caactgtgat ggaaaaatcg
    46741 ttgatgatct tattcatgtg actccttaat ttatgtcggg cgatgggcga gcaggtcaat
    46801 gtgtgtgatc agcgctttgc gccccgccgc gtaatttcca ttgcaaatac tttgaaccat
    46861 ggttttatgg tatccccata cttcttcgag ataatcaatg ccgcctgcgt acatgttcag
    46921 gccgaccgcc tcgtatgcat tccaataggc ctcgagcaag cccgtaacaa agggattatt
    46981 gaggcggctg taaatcagca tatgcagctt gcgatgctct tcgtgtggaa cctgcacttg
    47041 ttttgcatgc aatttttcga aggctcgggc ggtgatgttt tttaattcca gcttgtcttc
    47101 ctcggttaag agcgataccg cttcatccca atatgcggtt tcgatgtgat tgcgcaattc
    47161 cgaatacttt tcgaagttgc tttgatcaag ggttaatgca tatgcaaggc tcttcatcac
    47221 cgatggggca aaagagtagg gtaggcgtct tgtgcccgta cggggacgaa cttcaaccag
    47281 acccagggct cgcgcaactt ccaattgttc gcgtaaggcg gcaaggctga tgcccaactc
    47341 gcggcttagg tcgtttaacg atggaagtcc gccagtggct tcttcatgtg aagccagata
    47401 gcgcaggaat tcagagagtt cgggagagat gcggtcgcgt aaaaccatgg ctgtaatctg
    47461 attaatcaga ttaataagat tttacgacgc gtattgggaa aagtcaagta tttgtgagtt
    47521 gcaaatgtga aagttgtgga tcaaggaaaa gaaaacgact tgagtgaaaa cctcaagtcg
    47581 ttttcgtaat tggcgctttg atttggttag ttattcagtg cgcctgcgtt gatccaatca
    47641 atgatgagct gaatttgtgc agggagtagt ttttgtccac gctttggcat ttcgccttcg
    47701 acgatctgtt ctaccaggaa actgtcattt gcattgccag gggtgatgac actgccgttg
    47761 aatgatcctg ccatgagggt ttcatacgtt cgcaagtcaa gaccttcttt gacctgttca
    47821 acaccgtgac atttggtaca gctggcttcg aaaatgggca tgatatcttt tgcgaagctg
    47881 accgtggcgg ctgtttcctg cgtttgggct ggtgtctctg cctgaagaat cggtgcttcg
    47941 gttggggcag ggatagcggt gtcagtagcc acagggatgg ttggtgcttc ggtgacagtg
    48001 gcaggtgagg gcggagtctg agtcgcttgt gtcccgcagg cagcgatcaa aatggtgaag
    48061 agggtcaaac tgaaaattaa agccttgttc atttctaagt tcctttcatt gtttgcgtgg
    48121 ggcaaacttt aactgattat caggtgaatt tgatgtggaa atcatgtggt ttcttactct
    48181 ggcggctttt tttcaaaaat atagatagta tctgcaaggt aaggtataat ccatttatca
    48241 cgcgtaaagc gtaaattcat ttcaaggctg agtatggcag gcgcattttc actccccaac
    48301 gaatattggt tatccattca gattactcca caagatgttg aaaatctgca taattatctt
    48361 tttgagcgcg aaacaccgct gacgacgcgg gatctttccg ttgaacttgt tgaaacacgt
    48421 atcaaggccg agcgtgcagt ggctgaggga aagaagaagg ctgcgggcag gagttatctt
    48481 ccaaaagaga agtatcaagt tggtgaagaa cttatattcc ctgcattaga ctggaaacgc
    48541 gggacggtta ccgcgttgcg tgaaggggta aacccttctg taacgggttt tgatgtactc
    48601 acggttacga tggaagatcg tgtcgagcgc atgttcgcag cgaaccttga agcccactac
    48661 ctgaacgatg ctccgattgt tgagcctgag acgaacgaga tgagcgcaga gatggtcttg
    48721 cgcgaatttg gcgagcagat cgaaaagaaa cttgaagcct cgtttaccgc tgatagcggg
    48781 ctggttaaga ttgccgggag ctggttcccg cgtgcgcttt tgatcgatat taatcaggga
    48841 cagttgaatc ttgctgaggc cgttttggat atggctggtg gtgagcctct taccactgaa
    48901 gcactgatga aggatattga actccctgca agtgttaacc cgaaattggc cgagttctcc
    48961 ctgaattacg ctttgcagag cgatgaacgc tttgacgagg tggggccggc aggacaggtg
    49021 ttgtggtgtt tgtatcgtct ggagcctgat ttggtccgtg aagtgccatg gccgcttcgg
    49081 tattcagaga ttgagcatga tcgcagcgtg cttggcgatg aaatcaaacg cctcgaagac
    49141 cagttggatg atgaattgac cccagtggac gaagatgatg cccgtggcga tatttcatcc
    49201 attacgatta ccttaattta cccgcacctg cgtgcaggga cgctccccat gtcagcccgg
    49261 gcgcgcaggt tgttcccaac agcatatgaa tcatcgcgtg tgcgttttac ccttgtggat
    49321 ggtaaaacca agcaaagtat ccctgcctgg gttgtattga agcatggata tgtattcggg
    49381 ttgcgtgaat ggtataaagc ccatcaactg atcccaggtt cgatcgtaca gttgaagcgc
    49441 ggtgaaaagc ccggcgaagt gatcgtggaa gtaaaaagcc agcgatcctc aaaggattgg
    49501 gtgcgtacgg tgctggtcgg caaggatggc gggtttgttt ttgccatgct gaagcagccg
    49561 atcaccgcag agtttaatga ccgcatggtt atccatgtcc ctgatttcaa atccctggac
    49621 cctgtttggg agaagaaaca gtcgttcaac gaacttgttt tgtccgtcat gcgcgaacta
    49681 accaaatcga acccacaggg acacgttcat gcgcaggaat tatatgcggc gatcaacctc
    49741 gtacgccgtg ttccgcctgc cccgcttttt gcattgttgg aaagcaaccc tgccttcaaa
    49801 catgtaggcg acctgcattt ccgcctggat gaggaggccg aatgagttta ctaaaaccga
    49861 acgctaaaac accttttcat attgactttg aatggtggaa gaaaaacgaa cgcgattggc
    49921 atgtgttttt acgttctctg ttgtgccccg aacatcagga ggcctttgcc gagatggacg
    49981 aagggggcat gatcgactgg atcgacccgc aaaccgctga agtacggcag gtggctggcg
    50041 tccaacatgc cctgatgagc cattgtgcct tgctgcccga gttttccgat gcacgcaccg
    50101 ccatggtgga agccgtattt cgaacctttc ttgtaaatgg caataccccc atgtctgccg
    50161 aggaactgag ccaaaaactt ggccgccctg cagacactat attgaagacg atcgcaggtc
    50221 cgcgagtgta tcgtggtttg agaccctttg aggttggcct accaatcgcg gaatcaggcg
    50281 agtagtttta aaacgcctcc gtagctcaat ggatagagca cctgccttct aagcagtagg
    50341 ttcccagttc gaatctgggc ggaggcgcca catggttcca tcaaacgcat agtgaaggag
    50401 agaatttcca tgcctgcgat cgtacgtaaa tccagcgcag cgttgttgga gacgcgccgt
    50461 gaaatccttc atgcagtgag ttggcatgtc cgctcaaatg tttggagccc tcccactgac
    50521 caatacgaaa ccgaagatac ctatgtggtc cgcgttgaaa ttgcgggcat gaatgaagat
    50581 gattttgagg tttccgtaga gaacaacaat accctgctga tcgcaggggt gcgtaccgat
    50641 gcccaggaac ggcgcgccta ccatcaaatg gaaattcggt ttggcaagtt tgcaaccgca
    50701 ttaggcctgc ccggaccagt ggatatcgaa cacgcacttg cagaatacaa agacggattt
    50761 ctaaccgtca ccctcccaaa agcaacacca aaacagatca aggttgaata aacatatgcc
    50821 cgcagaacgg tggcaaaccg gaatcaatga gtttttacaa tccatgggcg agaccgacga
    50881 agaaatgata aaaatgttca tgacttcggc cctggcacgt tttcaaaaag acgatggcgc
    50941 ttcacacgcc ccgcagcctg atgacaaaaa cgagggacag aaattccccg acacacttcc
    51001 cattctgccg ttgcgcggag tggttgttta ccccaacacc gccgtcccat tgaccgttgg
    51061 ccaaccccgc tccatcaaac tggtggacga tgttgtcggc ggcgataaat tggtgggtct
    51121 cgtggccgcc ctcaacccag agctggaaac ccccggtccc aacgaactgt atcaaatcgg
    51181 aaccattgcc acggttcatc gcctgctccg tgcccctgat ggaaccattc ggctgttggt
    51241 gcagggcatg gaacgttttc gcgtggagga gttcgtcgcc gacgagccct acttgaaagc
    51301 acgcatcagc ctgattcccg aaaccgtgga atcgaatctc gaaaccaacg cactggcccg
    51361 taacgcccgc gaccaatttc agcagatcac acaaatgatc ccctcgttcc ccgatgaact
    51421 ggtgggctca attacctcgc tcgaagaccc tctgcaaacg gtttatacca tcgccaattt
    51481 tcagcgcatc aatttgcagg atgcccagga gatccttgag ctggattcag tgtatgaaaa
    51541 attgaaaaag ctcattggcc tgctcgtccg cgaggcggaa gtgctgcaga tcgggcagaa
    51601 gatccagaac gaagcacgcg gtgagatcga aaaagttcag cgcgaatatt ttttgcgcga
    51661 acaaatgaaa gccattcaac gcgaactggg cgaccgcgat gagcaatctg tcgaagcaga
    51721 tgaattccgc aaaaagatcg aagaggcgaa catgcctgag gaggcccata aacaggccaa
    51781 acaggagttg gatcgccttt cccgtcttcc cacggccgcc gcagagtatg gcgtcatccg
    51841 cacctatctc gactggctcg tttcgcttcc gtggtcaaaa tccacgcagg ataacctcga
    51901 aattgcgcac gcccgtgaga tcctcaacaa agaccatttc ggcctcgaag atgtgaaaga
    51961 ccgcattctc gaatttcttg ccattcgtaa attgcgtatt gaccgcaagg acgatgagaa
    52021 agccccttca gacgatctga ttcgccgtga acgcgaaggc gtgatccttt gctttgtagg
    52081 cccgcctggc gtggggaaaa catcgcttgg gcaatccatc gcccgtgcaa tggaacgcaa
    52141 attcatccgt gcgtccctgg gcggagtgcg tgatgaagcc gaaatccgcg gacatcgccg
    52201 cacttacatc ggcgcaatgc ccgggcgcat cctgcaatct ctccgccgcg ttgattcgaa
    52261 aaatcctgtt ttcatgcttg atgaagtgga taaactcacc tacgattttc acggtgaccc
    52321 cgcatcggcc ctcctggaag tgcttgaccc ggaacaaaac agtgaatttc gtgataacta
    52381 cctcgaagta tcttttgacc tatcgcaggt tttctttatc acaacagcca acacgcttga
    52441 aaccattcct ggccccctgc gtgaccgcat ggaaatcatc ttcctttcgg gttataccga
    52501 aaacgaaaag atgtccatcg cacgcggtta tttgattccg cgccagattc gtgaaaacag
    52561 cctgcgcgca gaagagatct ctttcaccga tgatgccctg cgtatgattg ttcgtcagta
    52621 cacacgtgaa gctggtgtac gcaatcttga acgcaagatt ggcgcagtct gccgcaaagt
    52681 gggtacgaag atcgccgaac acaaaggtga aaaatttgag attacgcccg agctggtgga
    52741 agaatacctc gaccatccca tctttttagg taccgaggaa ctcaataaac gcacctccat
    52801 ccccggggtg gttcccggcc tggcatggac ctcttttggc ggcgatatct tatttatcga
    52861 aacaactgcc atgcccggtg gacgcggctt tcaggtaaca ggttccattg gcaacgtcat
    52921 gcaggaatct gcccgggctg cactctcata tgtccgctca cgggctgctt ctctaaacgt
    52981 gcccgaagaa ttcttcaaca aatatgacct gcacatgcac atccccgcag gcgcacaacc
    53041 caaagacggc ccatcagcag gagtggcgat ggctacatct ttggtgtcac tgatctctgg
    53101 ccgcaaggtc aagccacaga taggcatgac cggagagatc acattgcgag gtcaggtttt
    53161 agccattggc ggcgtcaagg aaaaagtatt ggcggcgcac cgcaacggtc tgactaccgt
    53221 gatccttcca aaacgcaacg aaaccgatat tgacgatgtc cctgacgaga tcaggcaatc
    53281 catgaaattt atttttgccg agtctgtcga agatgtgatc aatgccgcat tggaaaagaa
    53341 caagcccgag gaaaagaagg ccggtaagcc gcgcagaaaa aaatcccaac ccacgaaagt
    53401 aaagaaaaat ggcaaaagta aaagtactaa tcgccgaaga tgacatcacc atggtatccc
    53461 ttttgaaaac cctgctcaaa atggaaggct ttgaagtgct tgctttgcag ccagatgccg
    53521 atgtgccttt agctgtgcga aacgaaaagc ctgatatcgt tttgctcgat gtgcatctct
    53581 tcggccaaag cgggctggat atcctctccg atatccggga agcgacagag acagccacca
    53641 cacgtgtcgt gatgtcgtca ggctccgatg tccgcgaaga atgtgcccgc cgtggcgcaa
    53701 atggtttcct tatgaaaccc tacatgcccg atgaccttat cacaattctc aagcaggttt
    53761 tgcaagccgc ctaaccttta cggtggaaag caaatatggc gattcgaatt ggaatcatga
    53821 cactctccga ccgttctgca cgtggagagc gcgaagacgc ctccggaccc gcccttgcgg
    53881 atcttgtcca cgctcaaggc tggtcggtcg tcgaacaagc catcttcccc gacgacgaac
    53941 ccgccatccg taccgccctg gtcacctggg ctgacagcgg acgtttcgac ataatcctca
    54001 ccactggcgg cacaggcttt gccccgcgcg atgtcacccc ggaagccacc cgcgccatta
    54061 tccagcgtga ggcaccgggg ttggccgaag ccatgcgggc cgatagcctg aaaaagaccg
    54121 cccatgccat gctttcccgc gctgtagcag gcatccgtgg acggaccctc attgtcaacc
    54181 tgcctggcag ccccaaaggc gctgtggaaa atttgcaggc catcctgcct gttcttcccc
    54241 atgccgtgca gctcctcagc aatgaccctg cttcagaagc ctcccattag tggagcgcac
    54301 ccgctcctac agaccaacct agccacgatt tacaactcat tggtataatc tgcccgctct
    54361 cgtataaaaa aacaaaatca attaatcaag gaaagtgcaa gcatgataga tgtaaatgaa
    54421 cttcgcaagg gcgtcacatt cgaattggac ggcaacctcc tcaaagtgtt ggattacagc
    54481 cacaacaaaa ctggccgcgg caatgccaac atcaaagtga aggcccgcaa tttgcttact
    54541 ggtgccaaca ttgagcgcac attcaactcc gggcaatcgg tgcaggatgt gtccctcgat
    54601 tttgccaacg tatcctattt atacaacgat ggcgatctct actactttat ggataatgcc
    54661 accttcgaac agcccggaat caagaaggaa tccctcggcg atagcgccaa attccttacc
    54721 gaaggcatgg aagtcaagct caccttctac aaaggtgaag ccattgatgt ggaactgccc
    54781 acctcggtcg acctgaaagt cgtcgaagct gagatggcca tccgcggcga taccgccacc
    54841 ggcgtcacca aaaaagtcat taccgaaact ggtgtggtcg tacaatgccc caacttcgtc
    54901 gttgtcggtg atgtcatccg cgtagatacc cgcactggtg attacgtcac ccgcgtgtaa
    54961 acctgcctca ggcttgccct gaaccccaca ctgataccca ttgcaaatca caggagcgcg
    55021 gatttggtcc caggcacagc acagactgaa acttgcgctc cgttttttta tataaaatga
    55081 aaactcccgc agggcgtgaa tgcccacatt tttatggcaa ttactttcgt ggccgaaacg
    55141 atgaagaatg tcggctgctc aaactgcacg gtcaggcctg gacgcgtgac ctgtgcaaaa
    55201 cctgccctgt gcctgaaatt gcccgcgcaa actcatgcca gcacatgcaa ttgaaagtca
    55261 cagtggaacg tcccatcctc gccatgttcc agcggcgggt gcaggtgagc gcattttgtg
    55321 aaaaatcaaa tcaaaatgtt tccgagccgc aaatcggctg tggaaaatgc cacgatctgc
    55381 cctttaaatt tgaaataaaa gaatgagcat ctgactccgc gggggagtcc aagaatatga
    55441 caaagccaat cctcctgctc gatctcgacg acactctgct caacaccaat atggaagcct
    55501 ttgtaccggc atattttcaa gcccttgcaa aggaactgag cacgtatgta accccaacgg
    55561 ccatgctgcc tgccctgatg gctggcacca atgccatgaa tgtgagtgac gatttttccc
    55621 gtaccctgga acaggttttc aacgcagaat tttatccacg catggatgct tctcccgaaa
    55681 cgctccgccc gcctcttgaa aatttttacg accatgtctt tccattgctg gggaacgtaa
    55741 cctcgcaaag gccggaagct cttgagttcg tacaatgggc gctttcaaaa gggtaccgca
    55801 tcgccattgc caccgatccg ctgtttcctc tcaaagccac tcattcacga ttgcgttggg
    55861 ctgggtttga gcctgagcaa tttgagatcg tttcatcctt tgaacatttt catttttcga
    55921 aaacgcatcc cgcctattat gctgaaattc ttgggcgtat gggctggccg gaagttcctg
    55981 tgttgatggt ggggaacgat attgaacgcg atatcaagcc atcgaaaatg cttgggctgg
    56041 ctgcattttt cgtggaaaat gaacccgctt cccagtctgg acctgaagcg gggctgcgcg
    56101 gcaatttagt tgatctgcgc cgttggcttg agtccactga cctttcggcc ctgatcccat
    56161 ctttcaaatc gaaggatgcc gtccttggaa ttctctctgc aacccctgct gccatgaaca
    56221 gcctgctaag tggtttggat gaaaccacct ggcctcgcga acattcagat gaagattggg
    56281 cgatgatcga aattatctgc catatgcgtg atacagagcg cgaaattcac cagatgcaga
    56341 tcaggttgtt tgctgaacag agcgaacctt ttatcccgcg cccggatacg ggtgtgtggg
    56401 ccagccagcg tgattatttg catgaaaacg gccctcaggc cctaaaggaa ttcaacgatg
    56461 cgcggcgcga aacattggcc ctgctctccg aggtttctgc cgaggcatgg ggccgcaggg
    56521 ctcgccatgc cattttcggc cccaccgatt ttctcgaagt tgtcagtttt atggccgatc
    56581 atgatcggat gcatctacag caaatatgga caacgctgaa gaaactctaa tgtttcggcg
    56641 tgtcttccac cccgccatag tgttatatga agacgaggat aagtgtggaa cttgtcctcg
    56701 tcttttgatt aaactttata aaattgctgc ttgaattcaa ggagaactag aatgagaaaa
    56761 cgcgttgttg tcaccgggct gggttgtgtc agtccggtag gaaataccac aaaagataca
    56821 tggcaggcgc tgctggaggg aaaatccggt gcggctccca ttacaacctt tgatgcgagt
    56881 gcgcataaga caaagtttgc agcagaagta aagggttttg atgcggctgc gttgtttgga
    56941 acgcgcgacg cacgcaaaat ggaccgcttc acacaatttg ccacagccgc cacattggaa
    57001 gcgatggcgc aatcaggcct gaaagtggac gaatcgaacc gcgaccgtat tggcattttg
    57061 atcggaacag gcatcggcgg tatcagttcc cttctggagc aatatgacat catgcgcgaa
    57121 cgcggccccg aacgtgtcag cccgttcctg atcccaatga tgatctcaga tggtgcggct
    57181 gggaatatag ccatccgtct cggcgtgcgt ggaccgaata tgtctttggc gaccgcctgt
    57241 gcctctggca cgaacgctct tggtgaagcc gccgaaatga ttcggcgcgg cgcggcggat
    57301 gtcatgatcg ctggtgcttc tgaagctgcc atcaatgcca ttgccatggc aggcatgaat
    57361 tcaatgaccg ctctttccac acgaaacgat gagccccagc gggcttcccg tcctttcgac
    57421 aaggaccggg atggttttat gatgggtgag ggggcgggtg tcctcatcct tgaatcgctg
    57481 gaatttgccc aggcgcgtgg cgcaaacatt ttgtgtgagt tcagcggata tggaaccacc
    57541 gatgatgctc atcatatttc cgcacctgct gaagatggcg ccggggcagt tggttcaatg
    57601 agccttgcca tggaagatgc cggtctgaag ctggaggaga tcgattacat caacgcccat
    57661 ggcacctcca catatttgaa cgataaatct gaaaccagtg cgatcaagac cgttttcggt
    57721 gaacgcgcct acaagattcc cgtttcatcc accaaatcca tgaccgggca cctgctcggg
    57781 gcttccgggg ctgtagaggc tgttttctgt gccatggcaa ttatggaaaa tatcctgcct
    57841 cccaccatga actatgaaac ccccgaccct gtgtgcgatc ttgattatgt tcccaatcaa
    57901 cctcgcaaag ccgagccgaa ccacgtcatg tccaactcat ttggatttgg cgggcataat
    57961 gcaaccctta ttctaagccg cttcaacaag taaaggagaa agaatgccat tcgcgcatgt
    58021 tacaggttgg ggcatgtatg ttcccgaacc tgtcctcacc aatgatgata tttccaaaat
    58081 ggtcgatacc aacgatcagt ggattcgtga gcgtacaggg attcgtgagc gccgcattgc
    58141 ccgtgagcat gaatttccat ccacaatggg tgtggaggcg gcggtcaagg ctttacaggt
    58201 agcaaacctt gctcccaccg agttagatct gatcatttgc acctcttctt cgcctgagta
    58261 tattttccct gccacggcct gcatcattca agaccagatt ggagcaacca aggcgggtgc
    58321 atttgacctg caggctgcct gcacaggctt tatttttgcg gtcaatatgg cggcccaggc
    58381 gattcgcagt ggatcgatca agaacgccct tgtggttggg acagaagtgc tctcgcggtt
    58441 cgtaaactgg aaagaccggg gtacctgtat tttgtttggc gatggtgcag gtgcctttgt
    58501 tttgcaggcc agtgatgtgc ccgggggcgt gctctctgcg gtgatgcatt cagatggttc
    58561 cggtgcagat acattgaccc tgcctggcgg cggttcgcat ttccctgcct ctgaattaac
    58621 cattcatgaa ggcaaacact tcgtccacat ggatggcaag gaagtgttcc gctttgccac
    58681 ccgcgtgatg ggccgcgctg tgatggaatc tctcgaacaa ttgaaaatga ccacagacga
    58741 tatccattgc attattccgc accaggccaa ttatcgcatt attgaaactg cggccaaata
    58801 cctcaaaatg ccactggata aattcgtcat taatgtagat cggtacggaa atacctccac
    58861 cgcttccatt ccgattgcca cagtggaagc cattcaaaat ggacgactga agagtggtga
    58921 caaagccgtg tttgtcgggt ttggcggagg cttaacctgg ggtgccatgg tcattcaatg
    58981 gacaggcccc atacccgcaa agaaaaatgt tcaccccgaa caatatcgct actttgcccg
    59041 cctgcgctcg cttttgcgcc gcgtctggcg ttatattgaa ggtttcttct cgcgtaaatc
    59101 cctttaattg ttttttcatt caactgtggt acgataaggt tgaaaatcaa aaaaggagtg
    59161 ttttatgcta ggactaccac gtggtgcaga gtggatcatc atcctcatta tcattgttct
    59221 gctgtttggg ccaggtcgca ttggcaaagt ggcaggggaa cttgggcgca gcatcaagtc
    59281 ttttcgtgaa gggttgaacc ccggcgaaga acaggctgct gatacggctg aaaagaagga
    59341 agaaaacaag taaccacaaa taaaggctcc taggtcagga gcctttattc tttaactgat
    59401 gttgtgggcg gttttatcac ctaagatcaa tagtccaatt ccctgatgtc cttatctttt
    59461 agcattaact gcacaaactt ttcctgattg ctcggagagc cgatcgtttt ccacacgctg
    59521 atgatttcgc tttccgattt ggactgatgg tgttcaagca cacgcaagga acattccttt
    59581 agagtctttt ccactttgtc tattttttgg aagcccgatg cgccgatcac gattcgatac
    59641 gagcgtgcct cacggacacg gtcaatacgg gcttcaatac gcggaaatac cagcagcacc
    59701 agcaaggtga tgacggtggc cgtaagtgag atgtacacat cacctgcgcc gattcccatg
    59761 cccaatgcag ccgaaagcca gatggtggcg gcggtggtca gcccgccgat cttgccgcct
    59821 tcacgtacga tcgcacctgc tccaaggaag ccaatgcctg taacgatgtt agccgcaacg
    59881 cgtgagcgcg tgaagcctgg gtccatggtt accgagaaga tcgtgaacaa agccgacccg
    59941 agtgtgatca gaataatggt gcgaaaacca gccgctttgt cttgatactc acgctctgca
    60001 ccgatcacac cgccaactac aaccgccagc aaaattttga tcaggttttc ataaaacatt
    60061 tccatgcgta ccgtccgttt ttatttgtgt gcgttcaaaa aagcatggct ttcaatcatg
    60121 ctttaagttg agcgatcagt acttcaattt catttgttgg ttgtttgcat acaaagcctt
    60181 cgcaaacata aacggtggct ttgccgttca gcagaggtcg gttttccaac agggcaggtg
    60241 catgctgtga tgggggatgg gaggatgcgg ccaccaccac gccgggcctg tattctgctc
    60301 gaatgacctt gagcaaccgc tcgaaatttt cctcccccgc ttcgcccaac accgcaatct
    60361 gtttcatcgt acccaaagca ttctccgccg ctgacagcca acgagcaaag ccaagcggat
    60421 atcgcaaaat ggattctccg atcaaagcaa gcgattgctc ggcaaggctg cgatagcttc
    60481 cctgatctgt ataagcggcc atcttcagca aggcttcaca agccaacgca ttgccagacg
    60541 gggtggcatt atcactgatg tctttggggc ggaataacaa ggtttcgcct gtgtgcggtg
    60601 tatcgaagaa tccgccatct gggttgctga acttttcgat catttcatcg gttaattcat
    60661 gggcgatggt aaaccatttg ttgttgaaat cggtttggta tagctcaaga agccccaaaa
    60721 ttagagcggc gtaatcttcg aggaaaacct cttcggttac ttgtccgttg cgccaggaac
    60781 ggtgcagctt cccattgaca cgcaagttat tgactaaaaa ctctgcgctg cgcgtagcca
    60841 gatcaagatg tgtgtgggaa tgcggggagg atcctgcgag gactcgggcc gattcggcga
    60901 aagccgccag catcagacca ttccatgcag tgaggacttt gtcatcggtg ccggggcgga
    60961 tgcgagaggc gcgggccgat aaaagcctgg agtgagattc tgccaatttg gcgggaacag
    61021 tttcagggtc cagtttgaag cgggcggcga gtgttgtgtc atctaatgcg cgttgcagaa
    61081 cggttttgcc ctcccagttt ccctgtgggc taattccata tgccgcttcg aaaaactctg
    61141 aatcatcctt caaaacagca cggatctcat cgagagtcca gacatagaac ctgccctcct
    61201 cgccttccga gtcggcatcg agcgaggagt agaagccgcc ttgttcgtgg gtgagttcgc
    61261 gagcgacaaa cccgagggtt tcttcaacga tctgtttgaa gaaaggctct tttgttacct
    61321 gccatgcatg cagatatgcg cgggcgagca gggcgttatc gtaaagcatt ttttcgaaat
    61381 gaggcacccg ccagaagtta tcggtgctat agcgtgaaaa gccgccaccg accacatcgt
    61441 acatgccgcc gcgagccatg atgcgtagac aatggtcaat gagtttgagg tattcatcct
    61501 ttttgttcgc aatggaatga tgcaaaagga attcgagcgc catgggctga gggaattttg
    61561 gcgcgtcgcc ccaacctccg tatccccaat aataggcacc ttgcaacgcc ttggcgatgg
    61621 gttcaaggtg ttcctgtgtg atcgcttctg tatttttgct ttggttttgg tattgcaagt
    61681 gcccattgac ttgttcgccg accttttcga tctcggcgcg gtcgtttttc caggcgtttg
    61741 caaggcctgt gagaacatcc atgaaggcgg gcaggttgaa gcggcgtacg ggagggaagt
    61801 aggtgcctgc ataaaaaggc ttgagatcgt gcgaaagaaa gaccgacatg ggccagccgc
    61861 ctgagcctgt catggctacg acagcctgca tgtaaatgcc atccagatcg gggcgttctt
    61921 cgcgatctac tttgatatta ataaaatgtt cgttcatgaa ggccgcggtt tcggggtctt
    61981 caaaagattc atgcgcgagg acgtggcacc aatggcaggc ggcatagcca atgctgagaa
    62041 aaatgggctt gtcttcggct tttgcttttt caatggcttc gctcccccat gggtaccagt
    62101 ccacaggatt atgggcatgt tgaagcaaat aaggcgaggt ggcttgcgaa agtctgttca
    62161 tgatgcgata aattatactt taaggggatt aacattcctg atggggtatg tataatgact
    62221 gaaacttaac tcaaggagac gagaatgaaa aaatatagta ttttgcttgc gattgtggct
    62281 cttgttttag cttcactggc ctgccagacc atcatgggtg gcggcgacaa tggctttgaa
    62341 gcccccgatg ttccgcaaca gacggacggg gatgatgtgg aagtcccttc tgttgaacag
    62401 gatggcggcg atatcaccgt aggcggcgaa tcacccttcc ctgtgaccag cgacgctttc
    62461 aacgtcatca gcacccccca gtctgtgacg taccagacca atatgagcgg tgatgatgtg
    62521 atgaagtttt atcgtgatga attgggcaag cagggctaca ctgaagacac cagcatgacg
    62581 acaaacttta gcggtatgat tgcgatgttc ttcgtgaaca acggcaagac cattgtgatc
    62641 ggcggcgcac ctgtgggcga tggatccact tctgttacgg ttgcctatca gcaataggat
    62701 atttgaaaaa tgcggcatct tgaaaaagat gccgcatttt tgtttaattt tggcatgttc
    62761 cgttaagtgg ttagaagcct ggttcaacct taacccagcc acggacagag ttgaccagga
    62821 atattgcttt gcagtttgca agttcatcct tttgaatcac ccgttcgctg atttgccctg
    62881 tttcaagcaa atgagcgcgg aatgtacccc ccaacagccc acaggagatc ggtggggtaa
    62941 atttcccttc gtccgtctca atcacaagat tgccgatggt gaattcggtc aattcctctt
    63001 tttcgttgaa aagcagaatg tcatcgtagg ctggggcagg atacatgtct cgttttgtgg
    63061 ttttgtgaaa gagaaagaca ttttgcgaat cgatcggttt ttgagccagg gtgattttga
    63121 acggcttttc ttgcggatgg aagtcctttg cttctgaggt aaggccgccg aatcggtctg
    63181 ctaaaacccg cacacgtttt ggagaggtga agttctctgc cagtgttgca aggtgggtgt
    63241 gtagtgtctc cggggaaaac tcaaaaccga agtaagcggc tgaatcggcc atgcgctcaa
    63301 gatgtttttc gaacaaaaaa taaccgcttt ctggctgcca aagcagggtt tcaagcaggg
    63361 aaaagccttg cggacgctgg tttaatacac gggctttgag tagtgcctcg ctatattcat
    63421 cggtgctggt cgaatcccag acgattcctc cacctacgcc atattctgcc ttgttgtttt
    63481 ttctgtctat caatgctgta cggatggcta cattgaagcg ggctttgcgg ttcggggcaa
    63541 tggtgccaat gctgcccgtg taaatttttc gcggtgtggt ttccaactct gcgatgatgt
    63601 tcatggtgct gactttgggc gcgccagtaa tggatgcaca cggaaaaagc gcattgaaaa
    63661 tttcagccag cggcgctttt gtttttgcct tgacggtgga ggtcatttgc agaagtgtgg
    63721 gataggtttc cacagtgaac aactgaggga cgtgcacact gccgatttca gcgatgtgcc
    63781 caaaatcatt gcggatcata tccacgatca tcacattttc agcacggttc ttcacagaat
    63841 ggtgcagcca gtttgcaagc tcgccatctt ccttggtggt gcgtccgcgt ttgaccgttc
    63901 ctttcatggg gcggcctgtg agcatttcac cgtccagttc aaagaacaat tctggagaag
    63961 ctgaacaaat cacatgcctg cctgtatcaa tataggcggc gtatttattc tgggtctggg
    64021 ccatgaaaag aaagaattcc catgggtctt cggtaaattc ggtttgcaga cgcatggtgt
    64081 aattgacttg ataggtcttg cccgatgcga tttgttcttt tacttttgtg atggcggcat
    64141 tatatacact gcgttctgtg gtgggttccc aatgcaggtt tgagaaatct tttacagtgc
    64201 ttggcagttg cacagactga ggcggatcat acaacccgaa ccagagcaaa ggaaactcat
    64261 ctgattcaat gacgcgcagt gcactgtcaa acgcgggtgc ggcttcgtaa ctgacaaacc
    64321 cagccgcata ccagtgattc tcgttgacaa gtctctcgat ctccagtaaa gtctcgcctg
    64381 cgttgtgaat attttctgtg cttatcacac gatgtggttt tgaaaaatgc agccactccc
    64441 tgtcattcct caaaatgatt tcatcttttt tgatcaattt tgcctcaaca tgaaaaaact
    64501 gctgccaatt gtaccccgat ggcttcccgc gctcctgatg atgctggcca tctttgcctt
    64561 ctcttcccgc accagcaaag aactacccaa ctatggcgga tgggattact tcatcaaaaa
    64621 aggcgcccat gcggtcggat acggtctgtt ggcgcttgcc tatctggatg cgcttccgcg
    64681 tcgtaactac gctttggcct ggttactcgc cctgttatat tcagccaccg acgaattcca
    64741 ccaatccttt gtgcccgggc gccatgccgc gtggacagat gtgctggtgt tcgataacct
    64801 tggcgcaatg atcgcgttat ttgttcatta tcgcaagtcg cgcaatgtgg atttatgatt
    64861 cgaacgctac agaaattaca attggagaaa tcatgaaaaa caggatcacc cgcagagatt
    64921 ttttgaaagc cttatccatt gggacagcat ctgcgctggc cctttcctgt ggagctgtca
    64981 ctccgcagcc agaagaagag gagacatcag aacccatgcc aaaccaggat acttcaactt
    65041 ccaccagcgc cgatgtgctg gtgttggggg cggggattgc aggccttgcc gctgcccgta
    65101 cattggccga taggggaatg agtgtcattg tccttgaggc gcggaatcgc gtaggcgggc
    65161 gcatgtggac ggacacatcg cttggtttgc cgctcgatct tggcgcttcg tggatccatg
    65221 gtgtgaacgg gaatcccatt gcgaaactgg cgaagcaatt cggcgtggcg accgcaccaa
    65281 cagacgacga aaatggcatt gcttttgctt ctgatgggag tgaattgccc gacgatgaat
    65341 tcgcgcgcat ggaagcgttg ttcgaggata tttacgaaga ggtcgcagcg atgcaggaag
    65401 atacagatga ggatatgcct ttacaggagg cttttgacga ggtcattgcc agcagggatt
    65461 tatctgaagc tgatttgcgg cggctgaatt attatgccta ccttggcacg gcattggaat
    65521 acggcgcaga catgaaagac ctgtcgcttt gggaatggga tcaggatgaa gaatttgacg
    65581 gggaggaagt catcttccct ggcgggtata accagatcac agacgggctt gcaaaaggtt
    65641 tggatattcg tttggggatg gtggtgaaca ccgtccgcta tggggctgat ggggtggagg
    65701 tggaaacttc gggcgggagc tttgtggcag ataaggctgt cgtgacgttt ccgcttgggg
    65761 tgttgaagca ggcttcggtt aagtttgatc cgccgcttcc cgagtcgaaa caaactgcaa
    65821 ttgggcgttt ggcaatggga gtcctcaata aggtttattt gaaattccct gaagcgtttt
    65881 gggatgaaag tattgagacc atcaattaca tgggcgaaac gctcggtgag tggtgtgatt
    65941 ggttgagttt tgcgcctttc acgggcgagg cggtgttgat ggcgttccat ggcggcgata
    66001 aaggttttga cctcgaagac ctttcagatg acgaaatccg cgaaggtgca atgaagacgc
    66061 tacgggtgat gttcggtgac gatattcccg agcctgaagg aatgcttgtg acgcgttggg
    66121 gtaaagaccc gtttgctttc ggcgcatatt cgcacattcc gccctttgcg agcggcgacg
    66181 attacgatgc gcttttcgaa cctgtggaag atgttttgtt ctttgcgggt gaggccacct
    66241 ggcgtgaaca ccccagcaca gtgcatggag cttatctttc cggtgtggca gccgccgggg
    66301 aaatttagtc agagaagcaa ttaaaaaaat acacctcttc ctgaatggaa gaggtgtatt
    66361 tttgaatgtc aggatgttat tggcaggcaa cggggacact ctgcaaagat tgtccattta
    66421 agtttccgcg tatggtaagt gcctctggat attgataggg accagttatg cgccaggtca
    66481 atgtttccgt tttggcgcta tcgaaggtca atgtgccttc agcttgcggc gcattgtagc
    66541 cctcaaaagt gccattaggt acctgtccgc tggcatcggt aatgtaaatt tgatattgcg
    66601 cggtggcagg cccattggat gagaattctg catacacaaa atatgttgtg cccgccgtgc
    66661 aaatggctcc tgacccttgt tcttgctcga ttcggagtga acctgtgact tgagcaacaa
    66721 cgggttgttg ttcgctgcct tgtggattgt tttgagccac ggcagtaagg gttgcattaa
    66781 cggcgctgct gatatcctgg gtcggtgcgg tctgcggtgt ttccatttgc gggctgtttt
    66841 gagccactgc ggtcaaagtt gcgttgacaa tattgctgat gtcctgggtc ggcgaaggtt
    66901 gagctccgca agccaaaagg gtgaaaaaca cgggtaacaa ataaaatatt tttttcattt
    66961 tttctcctgg gtaaatttga cgtattcgat tcttgatagt ttcccgcttt ttagagcatg
    67021 cgagaaaaaa caaaccttgt tgcgcaaatt cccccgtctg cattattcga actcaatgtc
    67081 aatttcctcg tcgtctttcc aatcttcgct gcgttcaaat tcaatgtcgc cgaagaggac
    67141 atcctgtttt gctgtcacac ggtagcgttt caccagttcc aatagcgcga gaaaagtcac
    67201 cacgatctcc acccgagtgg ctttatcggt gatcagtccg ctgaatgaca tgcgctggat
    67261 gtttttcaag gtcttggtga tgtagtcaat tttttcacgg atcgtcactt tgggtgtgct
    67321 gatcactgtg gcaagcggtt ttttctcttt gccgcgtgca atgcctgcct cggcagcggc
    67381 cagcaaacct tcaagggtta gattcgaaag gtcgagtttt gcctccactt tggggggcgg
    67441 ggccacgcgc aaatgtgtgc gtaaattatt ttcctgccgt tcggcaagcc agcctgcgat
    67501 ttccttgtag cgtttgtatg aaatcagcaa ttccaccaac tctacaccgg ggtcttcttc
    67561 gccaggctca cgttcgatcg gtcgcggcag caatgcttca gatttgatct gcaccagttt
    67621 ggcggcgatc acaaggaacg acgaaatttc gtcgggtttc atctgttcca gtccgtgcag
    67681 gtatgataaa tactggtcgg taactgaagc cagtgaaatg gcggtgatat ttaattcagc
    67741 gcgttcgatc aaactgagta aaaggtccag tggcccttca taaacgggcg tctgcacctt
    67801 gtatccgctg atctgatgtc ctagcaggtt ttccatggcg gccgaattat accagtgtat
    67861 aatcctgccc atgtccaagc ttactcacct cgatgaacag ggccgcgcgc acatggtgga
    67921 tgtgggtgcc aagcccgata cggaacgcac cgccattgcc cgcggtgaaa ttcacatgaa
    67981 aaaagagacc ttcgaactga ttcgtgatgg gcaggttaaa aaaggggatg tgctgaccgt
    68041 agcccagatc gcaggaatta gcgcctcaaa acggacctct gagctgattc cgttatgtca
    68101 tccgctcttc ctttcgaaag tggatgtgac cctcgtcctc gaagaatccc tgccgggagt
    68161 ggtcatcaca gccaccgcca aaaccacagg caaaacaggt gtggaaatgg aggcccttac
    68221 agcggtctct gtcgccgcat tgaccgtgta cgacatggcc aaagccgccg aaaaaaccat
    68281 gcgcattcaa aatatccgcc ttgtggaaaa acatggcggt caaagcggcg atgtggtgaa
    68341 tgaataatat ggaatactct cccgtcaatt ttcaagagaa gttttccagg ttctccgagc
    68401 agtggtcacc gaagatcatt gcccagttaa atgattacca tcttaaactg gccaaggtgc
    68461 agggagaatt tgtgtggcat gaccaccctg aaaccgatga ggtgtttatc gttatcaaag
    68521 ggcatttgga tattttattc cgtgacggca aggtttccct gactgaaggg gagatgttcg
    68581 ttgttccaaa aggtgtggaa cataagcctg ttgctgaaaa cgagtgccac atcttgctta
    68641 tcgagcctgc gggtacggtc aacacaggcg atgtaactga taaactcact gctccaaata
    68701 atgtctggat ataaaatgaa tcacataaaa ctcttatttt ttgccaccat ccgcgatcgg
    68761 gcgggagtga aatcccttga aatggatatc cccgccgacc tgaccattca aggtctcaaa
    68821 gacaaattgt cagtggaata tccgagcctc aaggaatcca tgcaatctgt cttgattacg
    68881 atcaatcgtg aatatgcctt tgatgaggct attgtcccgc cgaatgccga gatcggcatg
    68941 tttccccccg tctctggtgg atgacccgaa ccatgaattc ttccaacctt ccaacgattt
    69001 tttccatcac cgaatcagaa attgacctga acgacctgct tgcaaggatt acgctcacca
    69061 gcacaggtgc agccgccatt tttacaggca tggttcgggg tgtgaccaca cgcggtgacg
    69121 cccatgaaac cgaatacctt gagtatgagt cttatgtgcc gatggctgag gccaaaatga
    69181 agcaggtggc cgatgagatc cgtgcaaaat ggcccgtgat cgaggggatt gccgttgttc
    69241 agcgcattgg caagctgtac ccaatgaccc ccacggtatt gatcgcctgt accgcagctc
    69301 accgtgacac gggcgtattc gatgcggccc gctatggcat tgaccgcttg aaagagatcg
    69361 tacctgtctg gaagaaggaa gttggcccaa atggcgagga atgggtggaa ggcgattaca
    69421 tccccaagcc tggagaatga tatgattttg aattcaaaac gattgatcat tcgaaatttc
    69481 aaggactcag atattgagtc ctttcttttg tatcgcaata ttcctgaagt ggcgaaatat
    69541 cagggatgga agctgcccta tccacgcgaa gcggcggtgc agttggtgga tgagatgcgg
    69601 aatatagata gtccgcaggc ggggcgttgg ttgcagttga tgctcgaact tcgagatacc
    69661 catgaagtca tcggcgatat cggtattcgc cttaagaggg aagatccacg gcaggcggtt
    69721 atcggcttta ctctggctcc tgcacactgg cgcaaaggat atatgactga ggcggtcgtc
    69781 accatactgg gatttctttt cgatgtgctc aacctgcatc gggtttcagc cgattgtgac
    69841 acggaaaata tcgcttcctg gcgcacgctg gagaaggctg gctttcgccg tgaagcccat
    69901 tttgtggaga attacccaat gggtgatttt tatgccagtg aatacattta ctcaatattg
    69961 cagcgtgagt ggcaggcagg gaaaaccccc gggtagggaa tgttttttca acggaagcag
    70021 gccgcagtaa ggcgggatac gggttttcat ccgcgtgacg gataacagtt tttggggttt
    70081 tatatgaagc cagtcagctg gaattttatt atgtcaaggt atctggagga agaatgactg
    70141 aacgtatcgt gattacaggc atggggactg tgaatccact tggtttgaat gtgaaggaga
    70201 cctggagcaa tatggtggcg ggggtctcag gggttgcgcc gatcaccctg tttgattcgg
    70261 ggccgttaca agttcatatt gccgcagaag tgaagggttt caagcccgaa gaatatatgg
    70321 atgccaaaga ggcgcgccgc cgtgaccgtt ttgagcaatt tgcgatcacc gccgccaaag
    70381 aggctctttt tcaatcaggc ttgcaggtga cagaaaaaaa tgctgggcgt attggcgtga
    70441 ttatttcttc tgccattggc ggtttgcact cgcttcagga agccacactc acaaattaca
    70501 aggaaggccc gaaacgggtc agcccattcc tgattcccat gctgatgccg aacggtggtg
    70561 ctgggatggc ggcgatcgaa ttcggcatca aaggtccgtg cttttcggtg gcttcggcct
    70621 gcgcttctgg tgcagatgga attggtacgg cattgatgat gctgcacact ggcatgatcg
    70681 atgccgctct ggcaggcgcc gcggaggcga ccatcacctc cactggtgtg gctgcttttg
    70741 accgtgtggg ggccatgtct cgccgtaatg acgatttctc catgactccc cagccctttg
    70801 ataaaaaccg tgacgggctg gtgatgggag aaggggcggg ggtgttggtg cttgaacgcg
    70861 aggcagatgc gaaggctcgc ggtgcaaaca tccttgctga gttggctggt tatggcgcta
    70921 cggctgatgc ctatcatgtc actgctccgc acgaacatgg agagggtggt acagccgcca
    70981 ttatgatggc gttgtcttct gcaaaggcga atattcagga tgtaggctat atcaatgccc
    71041 atggtactgg tacgcttttg aacgatcagg ccgaaacccg cgccattaaa gctgcctttg
    71101 gcgaactggc atataaagtt ccgatctcat ccaccaaatc catgacaggg cacatgatgg
    71161 gtgccaccgg cgcattggag gcaattatct gtgtgcaggc ggttcgtgat ggcattttgc
    71221 ctcccaccat ccactacgaa acccccgacc ccgaatgtga cctggattac atccccaaca
    71281 aggctcgtga ggctgatatt tcgctggcca tcagcaatgc cttcggcttt ggcggtcaca
    71341 acgccgtgct ggcagtgcgg aaatacgcct gaaaataggc ttgtaggttc agaaaatgcg
    71401 tgctatactc ggcattcgca tttcataaac aaccaataaa acgtaaggaa gaacgtaaaa
    71461 tgacagactt tattaaggcc gttgaggcca aaccgaacga gaaaatcccg caaatgagtt
    71521 caggcgacac ggtcagcgtt catgtcaaaa tcaaggaagg cgaccgcgaa cgtattcagg
    71581 agttcaaggg aatcgttatt cgcctccgca agggcggcaa tgacgcttcc gttactgttc
    71641 gccgtatggc caccaacggc atcggcgtag agcgtacctt cctgctccgc tccccgcgca
    71701 tcgacaaggt agttgttgaa cgccacagca aagtccgccg cgctcagctt tacttcatgc
    71761 gtggccgcac tggcaagagc gcccgtctca agcagaaatt cgactaaaag caacagtcac
    71821 caccagggtt cttggaccct aaaggttcac aagaccttta gggtcttttt gttttcaagg
    71881 agttgtacca tgcccaaacc attgatcggc atcaccaccc gcaacggcaa ggatgccgac
    71941 ggacatccac ttactgcgct ccagcattcc tacatcaacg ccattgtgca ggcgggtgga
    72001 atgcccattt tgatcccctc aatgctttct gaggaagact ttactggatt gtattccctg
    72061 cttgcaggca ttctcttcac cggcggcggg gacgtttctt tggaatattt caacggttct
    72121 gcccacccga gaattggtga agtggataag agacgggata ctaccgagat caccctgatg
    72181 cgcgcagccg ttaacgatgg caagccattg ttgggaattt gccgtggcgc tcaagttatg
    72241 aacgtggctt tgggcggcac actatatacc catatccacg atcaggtaaa aggggcgctt
    72301 gaccatgatt accccggtga tctgcgcaag gtaattgtcc atcccgtaaa tgtagacgag
    72361 agcacccgtt ccgctgaaat ttttggtgag accctgctgc atgtcaacag cttgcaccat
    72421 caaggcttga aagatatcgc cccgggcttg cgcgtagcag gccacgctcc tgatggcctg
    72481 gtggaggttg tggaaatccc cgaccatcct tatgccgtgg ctgtgcaatg gcaccccgaa
    72541 tggctgacag accaacctgc catgcaaaaa ttattcaagt catttgtaga tgcggctgga
    72601 tgaatcaaag aacgatgaat tcagaatgat gaatttacaa aacaggcagg atgcaataac
    72661 ttcccccatc ggtgtttttg actcaggcgt aggcggatta tccgtcctgc gttccattcg
    72721 cgagcaaatg ccagaggaat ccgttttata ctttggcgat caaggacacg ttccatatgg
    72781 tccgcgctcg atggggcaga ttcaaagttt ttctgcaggc atcacccgct ttttgctgga
    72841 ccatggttca aaattgattg tcgtggcctg taacaccgcc tccgccgccg cattgaccta
    72901 tctgcgcgaa agctttcccg gtgtttcttt tgtcggcatg gagccagccg tgaaacccgc
    72961 tgcggagacg accaaaacgg gcagggtggg ggtattagcc accccggcaa cctttcaagg
    73021 tgcattgtat gcctcggttg tagagcggtt tggggcaggt gttgagttgt ttcaacatac
    73081 ctgcccgggc ctggtgagtc aaattgaaaa aggggaattg gactctgctg ctacgcgtgc
    73141 cattttagag gatgcgcttc atcccatgct tgagaaaaat atcgacaccg tggtgttggg
    73201 atgcacacac tatccatttg tgataccgct gattcaggag gtggtggggg gcgcggaacg
    73261 tgttcgagtg atcgatcctg ccccatcggt ggcgaagcag gccaaacgtt tgttggaagc
    73321 ggcagggatg aagaatcctg cggggagtcg ggcatccatc cgctttttta cttccgggga
    73381 tgttgaatcc atgaaatcca tgctgcctgt gttgttgggg gaaatgggcg atgtagaact
    73441 ggccgtgtgg cagaatgacg aagaggttaa ataaaaagac ttcggaattt ccgaagtctt
    73501 tttattgcta gggtcgaaat tctttttgtt ccacttcaat attggccgcc ttgagaaaat
    73561 tcaacgcgtt ttcatcggtg cgataggcag tgctgtacac gatgcgggtg atgcccgcat
    73621 tgatcagcgc ctttgtgcat tgaatgcaag gcagatgtgt gacgtaacag gttgctccct
    73681 tggtggagac accatgcagc gcagcttgaa tgatggcgtt tgtttctgcg tgaatcgtgc
    73741 ggacgcaatg accttccacc atgaggtgac cgacttcatc acaatgttcc tgcccggcgg
    73801 gggagccgtt gaagccggta gttagaattc ttttttccaa aacaagcaca caacctacaa
    73861 aagcgcggtc acaagtgcta cgttcggaga ccgcataagc gatcttcatg aaataggaat
    73921 cccaatcggg gcgcatattt tctccaggca ttatcagacc tgacaggttt tcaggacaaa
    73981 gatcaaacga agttctttgc tttgaaacct gccaggtctt tttactttta ttccactgtt
    74041 acgctcttgg cgagattgcg gggttgatcc acatccaaac cgcggatggt ggcaatgtga
    74101 taagccagca actgcaatgg gagtacggta atgatggggg agagcatcca cggcgcttcg
    74161 gggatccaca aaacgtggtc agccatgcct gggacgagct catcgccttc agtagccact
    74221 gcaatgacca tgccgccacg ggccttggcc tgctggatct gtgagatcat tttctcatgc
    74281 cagggatctt tgggggcaat gcacaacacc ggcatttcct catcaatgag ggcgatggga
    74341 ccatgtttca tttcgcccgc aggataacct tcagcgtgga tgtaggagat ctccttgagt
    74401 ttgagcgcgc cttcgtacgc gatcggcatg ttgatgccgc gtccaaggta gaggcagccc
    74461 ttaatgtctt tcaaggcatg tgccactttt tcaacctcgg gttcggtatc aagcacgcga
    74521 ccagcaaggt ctgggacaag gcgcaagtca gccacgagtg ccttgcgagt cttttcgtct
    74581 atgacgccgc gcatatcggc cagtaaaatc gcgagcatgt attgatccac caacggcgct
    74641 gtaaacgcct tggttgaagc caccccgatt tcaggtccag tttgcatcgc gacggttcca
    74701 tttgaaacgc gcatggcttg tgatccaacc gcgttgacaa tgctccagat gatgccgccc
    74761 ttgcgtcgtc cttcttccat ggcggcgagt gtatcggcgg tttcgccaga ttgcgaaatt
    74821 gcgagaatga cggtgttttc gtccacgatg gggtcactgt aacggaactc ggagccgatg
    74881 accacttcca ctggaacgcg tgcgatcttt tcgatgaggt atttgccaac catgcctgcg
    74941 taagcggccg taccacaagc tgtgatgtaa atgcgttgga tgcgcttggc gagttcgggg
    75001 gtgagattta attcaggcag gcgaatgcgg ccttctttga aatccacacg acctgcaagt
    75061 gtgtcggtca gtgcgcgcac ctgctcgtgg atttcctttt gcatgaagtg gcggtactcg
    75121 cctttttcag cggccacggc atcccacgcg atggtgtgga tctcaggttt gacctccact
    75181 ccttcaagtg tttcgatgcg tacgccttct cgggtgacga tcgccatttg acgcgactca
    75241 agaaaaataa ctttgcgggt gtgttccaaa atggcgggga tatcagaggc gatgaagttt
    75301 tcgttttcac caaggccgat caccacgccg cctgcattgc cgatgcgggc agtcacgatc
    75361 ttatcaggct catcagcaga catgagcact accacattgg ccccttcgat cttttgaaag
    75421 gtgcggcggg cggcttccac aaaaccagcg cctgggttgg ccgcctggta atgctctgcc
    75481 agatgcacga tggtttcggt gtcggtgtct gaaaggaaat ttacgccttc actgacgagt
    75541 tcgtctttga gttcgagaaa gttttcgacg atgccgttat gcaccaccac aaccttgccc
    75601 gaattgccca catgcggatg cgcattgcga gccgatggtg cgccgtgggt tgcccaacgt
    75661 gtatgaccga tgcccggcgc acccgtgagc ggggatttga ccacaaggtc aattaattgc
    75721 gacaatttgc ccgcgtctct tctaacttcg atctggttgc tgttaatgac agcgactcca
    75781 gccgaatcat agccgcggta ttcaagcctt ttcaggccat tgagaatgat cgacaccgcg
    75841 tcacgcggtc cgacatatcc gacgattcca cacatagtag gggttcctcc gattagtatt
    75901 agtttgatgg atatcattgc aatgcaagtt ccttccccga agaagcaatc tcttgaacgg
    75961 gagatcgctg atcagggaaa aggcattcac gcccttgcaa tgacagattg ttttcgccaa
    76021 tcccttgctt tttgaccctg tgatcgctca gagggtatta ttagcaaagt tatcttttgg
    76081 agggaaagat gtgacgggta tggcgtaccc ggagggcttc cgctgatcgt tcgatttttt
    76141 tctcttatag gctgaaacac cttttatgtt tttggtgtat caggcctggg agaattagct
    76201 ccatttcgca atggagaacc ctcgtcctcg tcaactttgt tttttccaac tggataccga
    76261 atgaagcccg tattccaaaa aacaaagtcc atgcgctgtc tttcccgttt tgttttgtcg
    76321 atgcacctcc tttcataggt tggcgggatt ataccgtaaa aatatcgccg acctgaaaat
    76381 ggtcggcgat atttttgttc atcagtaatt aagcatctgg gtcgaatgtc ggcgttggtg
    76441 ttggcgtctt ggttggtgtt ttagacggcg ttaaggtggg cggtggcgta tttgtctttg
    76501 tcggtgtggc cgtgttagtc ggtgtggccg tacgagtagc cgtagcagtc ggtgtgaacg
    76561 tccctgttgg ggtgcgggtg atggtcggcg tgttggtctt cgttggcgtg ctggtagccg
    76621 gttgggtatt ggtcggtgtg cgtgtaatgg tcggcgacgg tgtgcgtgac ggagtgagag
    76681 taatggttgg cggcagtgtt accgtgctgg ttgggacagg ggacggagcc ggggtaaggg
    76741 tgggagtgcg tgtgatcgtt gggctgggtg tgatggtagg tgtattagac ggggtgggtg
    76801 tgtagttggc cacagaacca gtcagaatac agtctcccca gcccgggaag gtaaatgtca
    76861 tcacaaccgt gtataaaccc tggaagttat tgtcgctgaa gtcggcatcc caagtatagc
    76921 gaccaccact gcccggaaga tcaagtgcag gcggggtgga actggtcaca atgggggagt
    76981 tggtattggc ggtgtttgcc gggttgtaat aagtggtgcc gttccatttg gtgaagtcga
    77041 aataccttcc gcctgtcaga ggggatggat cccatgttag ttcggtttca gtcaagtagg
    77101 ctgttgcgac gttgttatta cggacacgtg cttcaaaact gtcaccattg aactgggtac
    77161 ggtcaatgaa aatgctggca caggaaggtg ttggcgttga agtaggcgtc tttgtaatgg
    77221 tgggcgtata ggtcttggtt ggcgtctttg tgaacgtagg cgtcagagtt ttggttggtg
    77281 tacgcgatgg tgtaaacgtc tgtgttggtg tagaggtact agtgagtgtt ggcgaaggag
    77341 taatggtcgg tgacggtgta atggtaaagg ttgctgtatt ggtcggggta tcggtgggaa
    77401 gtggtgtgtt tgttggcgga ccagccagaa tgattggcgt gttgatggcg cgagttgcat
    77461 agaactgttc taccacgccc tggcggaagg aagccaagtg aatcatggag ggttcaatct
    77521 tgaaaactgg caacaccatg aaggcaaaat tgtaatctgc ggcaaccacc acgtattgac
    77581 cggggacgcc tgcaaactca tctgatacac caccaagggt acaactcgca taaacgggtt
    77641 ggcccattaa tgggggctgc cagatgcggg cagagcccgc cgtagaacac acggtgatat
    77701 tcaggtagtc attatctgcc tgggtgagga tttcataatc tggtgcagga ggattgcctc
    77761 cactttcatc gtcacgggta tcataatctt cctgcgaaat gccatagtgc aagccgataa
    77821 tgatgcggcg ggtttcatct tcgatggagg ggatgcgggc cagttcgatc tcgtccagct
    77881 cactggttcc atcgcataag gaaccatccc catccatttc gccgccggta cagtagattt
    77941 cctcaaattg gccagtgacg gcatagcgca caccgaaacg ggtggagttt tcaatgatca
    78001 accatgcata gaacagacgc ccaaattcta tggtacctat cagaaccagc aagagaatcg
    78061 gcaaaacaag cgcgaattcg agcatgcctt gtgcccgttt tcgccctgtt gctggcttcg
    78121 attttttgag taagcgtatg ttcataatga tacccttgtt agcggaatta tactgcctgt
    78181 tatctgatta ttgactaatt ctcgtaaaga tattgcctgc gattgcaagg aaaatcggac
    78241 ctaagtcaag gcctggatca tctgcataat agaattgccc atgatttgct tcacgaacaa
    78301 gtactccagt aaccgggtcg taaacgtcac cggcttcttc tgcaatgtac gtaagtaaat
    78361 ttgcggcgga atagggcaat ccattcttgt aattggtatc tgctttcgag tccaagcctg
    78421 cccaataaat agaattaacc aagccggtgt cggtgcctaa tccaattgtg aaaatagtaa
    78481 tgccattgcc ggtttctgga ttggcaagat catctgcagc atcccgtgca tagtcatcgg
    78541 cgtcatagtt tgcatctggg ggaacaggag ggacagcatc tggatttccc cggctatggc
    78601 gggtaattaa tggggtaatg ccgtcagcgg caaggtcgct atcacggcaa ggagcggttt
    78661 tgggagcact ccaggtactt gatggacaga aaccatcggg atgtgtcaaa agaaaattcg
    78721 catcagtgct tgtggcattg gctggaccgc ttaatagagc aataacaacc caaaatgcat
    78781 cattacgtcc tgaagtaata aatgcattat ttgcataata tagtgcgcct ccaatattac
    78841 tagagccaat ggttgtaggg ttaccttctc catctcctga tgtggctgca ttaagatctg
    78901 caccgcctgt gtagacgggc ctttgtaagc cggcgaatgt attagcagga tcatcgtttg
    78961 gatagtataa acataatcct tttgagggtg aaaaccattt gttagcagtc gaaggatcaa
    79021 catcacatat tggtggttcg taggctttta tgttagctag taggttgtcc gcttctgtaa
    79081 cgtcgtcttc aaatgggtac aaaatagggt cgcgagtccc gcctggagat tgtgaagtca
    79141 ttgtaaccac tgctacacgg tcataaggaa aaaacagaaa attattaaca aaacttcttg
    79201 cgaccgtttt gatgttgtcg agcggttggc acgcgcctgc gccgccattt gctacaaggt
    79261 tacagtctct cggattgtcg cctgggatat caaatttgtt tgcagcacct gtggttccat
    79321 aagtcatgga aagcgatgtg tccatgatta acaccaagtc gatagatgcg gcttcgccca
    79381 cagatgtggc ttgaatggtc gtgccattaa tccctaagac ccgcataaac ccgaaatcca
    79441 cgttgcgagt ggcggtcacg cgtaccagtt ttctagcggg ctggtcgtct gatgtatcag
    79501 gtgtgtccca tcctacaggg cataaagttg gatcatgggc tggagaaccg ccataagcat
    79561 tggttgggtc gcacattgtg atggacacat caacaacatc cgattgattc aacctgagaa
    79621 aattctcggc agcacttaca aggtcgtctc cagtaaaatc tttgcggaat tgctgggcag
    79681 cagctacaga agcggcgtca ataccacgct ttaatcgcgc gtattcgatc agcaagactc
    79741 caccatcggt catcaagccg atcatagcga tcaagccaac aaatgcaaag gcgatcagga
    79801 tgatggcctg accacgttct gattttttga gcagcttttt gatcataaat tcactctctc
    79861 ttcttcactg gcaccccgtg cctggaatag ggtcagctgc attagctggc atgatggaat
    79921 gcgcgcgtag catcaccttc cctgtataga acaggccaat cactgggcgg tacccataat
    79981 aaacttcaac aagtaccaat cccgcatcag gagcggtatc agcataagtg ttttcaatat
    80041 ctgctgtagt gaaagcagat gtaatcatgc tagggttggt attatatggg ccagtatctg
    80101 gataacgagt ggctgttgtt ccaccgacta cgcaatacac tgtaacaata acaccatctg
    80161 tagcaggatc aagaattagt ttgcgaccat cagttggcgg ggccaattcg gttcttacca
    80221 attcggcggt ggtgatgtag taatccatgt cgtcggtgcc gtctgcgtta agtg
//
LOCUS       DAOQLC010000005        64837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_93_length_64837_cov_23.659751, whole genome shotgun sequence.
ACCESSION   DAOQLC010000005 DAOQLC010000000
VERSION     DAOQLC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 64837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93_length_64837_cov_23.659751"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..771)
                     /locus_tag="PL000_01940"
     CDS             complement(94..771)
                     /locus_tag="PL000_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC87682.1"
                     /translation="MKFRTLRSIVRFVMNIIADVEVVGIEKLPQGNVMIAANHLGRLD
                     TAVLLYILDREDIIMAVAEKYRDHPIFGAIGRAANAIWLNRFEADFAALREILTRMQK
                     GGLMVIAPEGTRSKTASMQQGKLGVAFLASKSGYPVIPVALTGTEDGGVVANLKRFRR
                     SKIKAVAGDPFFIEIPKGSGREQAMREATDEIMCRIAVELPESYRGYYENHPRLQELL
                     AERSSTT"
     gene            complement(776..1441)
                     /locus_tag="PL000_01945"
     CDS             complement(776..1441)
                     /locus_tag="PL000_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC87683.1"
                     /translation="MRSFLRWLIPFVLNSFTTIKISGYENLPEKGGFVIAVNHLGFLD
                     APMAYYALDNWNLFIPVAEKWEENPLWRFLGRHLNFIFIDRFNPDLKAMREIWRRMED
                     GQTLVIAPEGTRARDEKMARGRPGVAYLASKKGWQIVPVAIAGTEDRIVLSNLKRFRR
                     TEIHLTAGKPFTLPSFPKENRDETLQEYVDEIMCRIAVMLPEKNRGYYAEHPKLKELL
                     SGK"
     gene            complement(1532..1918)
                     /locus_tag="PL000_01950"
     CDS             complement(1532..1918)
                     /locus_tag="PL000_01950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC87684.1"
                     /translation="MESEQRRLARRNFNYYMRVKEEASGKVLGHISDISTGGFRIDSS
                     QSVSLNIDMRLYIDQLGEISSKSYILLKARPMWCRQDQFDPNMFNIGFKLVDATPVDR
                     EIYMKMYEVYGDKTPPKTGELKFPPE"
     gene            complement(1997..3148)
                     /locus_tag="PL000_01955"
     CDS             complement(1997..3148)
                     /locus_tag="PL000_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HNC87685.1"
                     /translation="MRKIAVLGIGQTKIDEHWEKSLREIAGEAAFAALQDAGIEKVDS
                     LFVGNMLSPLINGQNQLGSFLADWIGLWKQEAVKIEAACGSGAAAFRAALMAVASGDI
                     ESALVVGVEKMTDKAGRDVTAALATAADADYEVEQGVSFVGINALVMRRYMHEYGWKH
                     EDFAQFSINAHANAMHNPYARLHEKISVEKFEKSSMVSTPINLLDASPIGDGAAAMVI
                     VPAEKVARKPRIVVEASAAATDSIAVHSRKDPLFLQAAYESSRRAYEMAGVTPADIDV
                     FELHDAFSIMAALSLEASGFAERGQGVRLALENEISPKGRVPVCTRGGLKARGHPVGA
                     TGVYQLVEVVQQLRGECGQTQVADAKIGMAQNIGGSGASILTHILRNDT"
     gene            3253..3738
                     /locus_tag="PL000_01960"
     CDS             3253..3738
                     /locus_tag="PL000_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87686.1"
                     /translation="MLRSYLISLFAAALFLAGCAQSTATAYPNPETGQNAISMPSLQG
                     DWRIKLTHSGGIMGLLRTMEISSDGSYTVTDERSSQTVTKTLGSEELQELTRLISSAQ
                     YIAPSGPNGMVCADCFVYDLEIKGSDSTFTIQLNDISLPDSGMAPVIAHLRDLIDKAL
                     K"
     gene            3741..4253
                     /locus_tag="PL000_01965"
     CDS             3741..4253
                     /locus_tag="PL000_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87687.1"
                     /translation="MDQTEWREKFLEAVANCCSEGKEAVEFIHVHKVRIGIKPARKSV
                     GAFWTLEKRAYLNSVYFSPESSLANPNAWVLLIHEVRHLQQGWSTALSIYGELDAWQY
                     QFNTLKKITSAKLPEAVDEILSLPLSMDRETLGRARKLMTQHAGKGYGANLLPLYPIH
                     MEIKYWISPD"
     gene            complement(4555..5718)
                     /locus_tag="PL000_01970"
     CDS             complement(4555..5718)
                     /locus_tag="PL000_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HNC87688.1"
                     /translation="MTNFNITPDRRKNFNRTKWTWYEKDILPMWVADMDFPAPKPILD
                     ALHKAVDHGVLGYEIPGTVLQETVAARMDRLYSWKFKPESVVTVTGIVSGFSVAARAF
                     CTPKKGIAIQTPVYNEFHQVKNNVGIPQTDIPLVKNVKGNLISYEIDWDLFEKRVKKA
                     GIFLLCNPHNPLGIIFSRKELTRMAEICIRNKVLIVSDEIHSELMLDGNKFTPMAKIS
                     SEIAQHTITLVAPSKTFNVPGLFCGFAIIPNKSLRERYTTEMEHMRLHVGSMGLHAAR
                     AAFSGKCDPWLAELQTYLTGNRDFLVEYVTRNFPGVRTTVPSATYLGWLDFTQTAIDG
                     SPYEFFKDKAKVALSEGKIFGNEGAGHVRLNFGTSRKILEQGLEKMQKAFSAL"
     gene            complement(5788..>6456)
                     /locus_tag="PL000_01975"
     CDS             complement(5788..>6456)
                     /locus_tag="PL000_01975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HNC87689.1"
                     /translation="VSAKNTLEVAMSELDYQTVQDYLASLVPSREAELLEMEAYAEKN
                     DFPIIGPACGYYCYQLARMINAKSIFELGSGYGYSTAWFAKALVENGGGVVHHTVWDE
                     ELSKRARKHLVKMGFADLVQFHMAEAVETLRQTEGPFDIIFNDIDKHAYPASLPEIKN
                     KLRSGGLLIIDNMLWHGRIFDEKDVSSATMGVQEFTRKITSDRDWIVSLTPMRDGMIV
                     AYKK"
     assembly_gap    6457..6556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6653..7846
                     /locus_tag="PL000_01980"
     CDS             6653..7846
                     /locus_tag="PL000_01980"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC87690.1"
                     /translation="MKYNFRLFWRTFYRSFFDSKNTPARLTRKRITFLILFYLVWPIG
                     GLIHWFCFALDDILFPAYKNHPIEKPLFILGNLRSGSTFLHRLLSRDTETFTSLTTWD
                     IYLTPSVTQKKFTQLIARLDNKYLGGTLHKLLYKFDAATLGKIKIHPISFFQPEEDEN
                     IHLHIWDGYFVTFLFPFMDEFPNYQHFDEALSPEHKKRIMTFYRSMIQRHMYANGKKY
                     FVAKNPAFSAKIETLAEFFPEARIIYLARNPLDMLPSTISWINYARRQFTDPGEGFFY
                     IEEIVDMTKHWYSHPLQYLDSHPSPRHLIMKYDDLIQRPETVIRAFYEQFGYPDKPGL
                     PVIIDQAVKETLTFNSDHAYSYESMGFTREQIVAMYPEVFKRFNFETREPFIESELMK
                     VERKK"
     gene            complement(7930..9348)
                     /locus_tag="PL000_01985"
     CDS             complement(7930..9348)
                     /locus_tag="PL000_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC87691.1"
                     /translation="MLKKKILIPIVIVVVAAATFFAFVAAGQNSLKEADAHFTAQNYA
                     EAAKAYERSARLLFWQTGLWEKAGIASAKGGDTAQAITYLDQAGDTLSEEGWGWLGSS
                     YLSLGEVDSAIAVLQRGLQTHEAPMLHGLLAFAYRQKKDWPAERAALEAQLRLDPNGG
                     DVYARYRLGLLLSVLEPEQALTDLMLASSLNPEFDPAVQTLRTALNLSATQPDASEQM
                     IHIGRALGLVQEWDLAVAAFEKAIALNAENAEAWAWLGEAKQQSAQSDAENIGLAELD
                     QAVLLDHESVTVRALRGLYWRRQEKYPQMLAEYLLAAEYDPTNPAWQAEIGNAYIKRG
                     DLVSALAAYQHATELAPDDPTYWRLLAVFCAENTIHMEDIGLPAAEKAVELAPNDPLT
                     LDALGWAYLSSGRFANAENIFVDLITRFPDHLQAHIHLALTYLSMGDRDAAFEKLNEV
                     KNMDPAGPYGQEAGRLLQLYFP"
     gene            9412..11769
                     /locus_tag="PL000_01990"
     CDS             9412..11769
                     /locus_tag="PL000_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87692.1"
                     /translation="MSPFTSQIFELLTVPINNLLYYIVLSYAVASAMLSAFIHWRASE
                     FPQARRALIGLGVLLAAQVTMFLFSGLGWQGLIDPKILPPMDRAFIIFGIIWITWLYA
                     FPEPSRPADAAAALLSVLVLTALGLSLLAWQPQITTITYNQTSDDWFWQIGSLAVGLI
                     GIAIMFVRKPDGMWNGIVLLGLGILGHIGHLAFPIDGNYSGVVRIAYMAAYPILLTLP
                     LRFSTPSSANESPARQITQKQDENLGERRRYSTDPKTFHAMLALAAETNPTKVSQAIT
                     RAIAQTMLSDLCFMIYINDNSNQMSIAGGYDLIREDALEGGTLNKGSIPMLANSLQRG
                     RPLRMPSSSTSADIKGLSDILGLTNPGHLLSVPILTAEKESLGGILLLSPYSDRTWTA
                     EDQAYLSNIATSLVPIIQRGQKVNRMEAQSSEAIRRSADLEHRVQELTEELHNAQTEI
                     QKAGSVEMASLLQAQEESQRIIEQLQLENAELRTGKSLQTGAAPASFASGQIEQELKT
                     TLQEVARLQNQLMEANMKTRELEKGHVTVKSTEQAEVVASIAQELRQPLSSIVGYTDL
                     LLGESVGILGALQRKFVERVKSSTERIRSLTDDMIQITTLEAELNDLNPEAVDLNAII
                     DNAMSYTSTQVREKNISMHLELPKQLAPIHADREALQQILIHLLQNAGAASPFEGTVR
                     LKVQTRNEENTEFILIQVTDSGGGIPAKELPRVFTRLYRADNVLIQGVGDTGVGLSIA
                     KTLTEAQHGRIWVESEQDVGSTFSVLLPIAGSAETNPKTKPKGKK"
     gene            11769..12599
                     /locus_tag="PL000_01995"
     CDS             11769..12599
                     /locus_tag="PL000_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC87693.1"
                     /translation="MQSMRDLRNALVVAMISIGLTVGSLSISLVEFAPQAVPSATNPL
                     QVVPPLLTATPTVVPSMTATLGLESPTPSPTSTFTATPPPPTTCPPPTGWTQLVVQAG
                     QTLDTIAQQYRTSKENLRAGNCLISDNLVAGSIMYAPAVMTSTVPACVPGAAGWKKSY
                     TVVSGDTLYAIATNHSTTAGLLKSVNCLSLDDIYPGNVLWVPDVPTRTPNPTPLPGST
                     VTPIPTQLLTETALPYTATTLPSVTPTEVPATPTPTTPATDTPTTAPTLTASPTPFPP
                     "
     gene            12653..14164
                     /locus_tag="PL000_02000"
     CDS             12653..14164
                     /locus_tag="PL000_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC87694.1"
                     /translation="MKSNSAFSTIALLAFLLSACGTPAEAVVQTPLPFVLITSAPNPS
                     PTPTPFQPSLNTPTQSAILSAAVDTSTPVPTVILPTETPSPTLIPPTESTATVDFNQL
                     FPTVAAPPVVEVVAVTPTALPPLTDNETVNFLLIGSDKRPGSSYRTDTLVIAIVWPKE
                     GQVSLISIPRDLWIYIPTVGMQRINTAYQSGEIGGYAGGGAGLLKDTITYNLGIRIDH
                     TAMVEFDGFRRIVDTLGGIEVPVACAYTDWRLIDPSLDMYDENNWWLYTVGPGQVHMD
                     GDLALWYARSRSKSSDFDRGRRQQEVLRTIFQKALQTDTFSKIPQLYEDFSSTILTDM
                     GLADMLLMSPYAVNFTNANIRGYFIRPPYVTSWTTPAGAAVLLPNETELNQMLLEATT
                     LSTFAEQRETITVEVQNGSPFDTMESLAASRLNYAGYQTILSNADRRDYANSVLVDFT
                     PGQDATQREAIIATLNLYTANVISLPDANSTVQYRVILGSDYQACFQPQDLSH"
     gene            14297..15196
                     /locus_tag="PL000_02005"
     CDS             14297..15196
                     /locus_tag="PL000_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC87695.1"
                     /translation="MAKTPDSAHISAKTLLPAAIQGWEMFMLDQGRSTNTVKAFLSDV
                     RLLTQFLPPDQSIGSITTEDLNRFFTWMEKDRGIPCSPKTLSRRITSVKSFFRWLHQH
                     GSLVLDPAEKVLQRSAISPLPQVLTDDEYEAVLLAADRLRRASKPDARSYTLVYLLLT
                     TGIKKSECLGIHINHIDLEAKNGPHVFIRYASPANRYKERKIQLAEDWLEAYKEYLAQ
                     YQPVDQVFAWSPRRLEYLLEDIGNEAGLTKHLSFDMCRWTCALRDYQSGMEADNIRQK
                     LGVSKIQWRELFIKIRQLNGEIK"
     gene            complement(15258..16703)
                     /gene="tilS"
                     /locus_tag="PL000_02010"
     CDS             complement(15258..16703)
                     /gene="tilS"
                     /locus_tag="PL000_02010"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC87696.1"
                     /translation="MMLENMDATLQDECGLVKDRPIIVGVSGGPDSLCLMETLRQAGY
                     SIIIAHFNHQLRPEAGQDAHMVEKIAARLMLGCIIDGADVRAYADQQKLSLEEAARNL
                     RYHFMFKLARERNAQAVAVGHTADDQVETILMHFLRGSGISGLKGMSHRSIIKTFDPK
                     IPIVRPLLNTWREETTIFCAANGLRPHYDSSNDSINFQRNRIRHLLIPSLESYNPKFR
                     DVVLRMSQSLKSDYSLLMETLENAWQEVVTSVEEDTIAFDADLLANRSIGLQRNLVKH
                     AMQILRPKLDVNFSALERATDLIKDRSHSSSVDLKGGLRMFREAGVLYVCTQNSKLPF
                     NLWPQMPSTTPVQVAIPGQVELAGGWRFSCERWHIPTLVREQAERNEDPFQIWLDAER
                     VPEMLELRVRHQGDTFEPLGLEGHSQKLSDFFVNEKMPQRARDHWPLLCAGDQIIWVP
                     GYRPAHSYRLTQSTKTAIYFSVSGPLEKITE"
     gene            complement(16773..17177)
                     /gene="mce"
                     /locus_tag="PL000_02015"
     CDS             complement(16773..17177)
                     /gene="mce"
                     /locus_tag="PL000_02015"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNC87697.1"
                     /translation="MPKIKAVNHVAVVVDDMEKSLLFWRDALGIQLHELRDVPAEKSQ
                     VAFLPVAGAEVELVMPTSDDSGIAKYLAKRGPGMHHLCLEVDDIEGMMSQLKAKNIRL
                     INEEPRTAVDGKKYAFIHPESTGGVLVELYQI"
     gene            complement(17200..17805)
                     /locus_tag="PL000_02020"
     CDS             complement(17200..17805)
                     /locus_tag="PL000_02020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC87698.1"
                     /translation="MEIRYLALGDSYTIGESVRESERWPNQLAAKLEEQGIHAETTII
                     ARTGWTVDELWEGIQVTPPEGRYDLVTLLIGVNDQYRGYPLDGYREDFRFMLGKAIEY
                     AGGDPQHVIVLSIPDWGFTPFAATRETEPISLQIDGFNAVNLEETKSAGAHYVDVTEI
                     SRMAMDDFELLAPDRLHPSGKMYSMWVEKVISVALEIVNRK"
     gene            complement(17962..18909)
                     /locus_tag="PL000_02025"
     CDS             complement(17962..18909)
                     /locus_tag="PL000_02025"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HNC87699.1"
                     /translation="MQAQANWESQLIALAGHFPHTSARPFFSYWAGDAQLHAAYKKAA
                     KITSEHSKSFYLASGLLPEEKRSAVRALYAFCRTVDDIVDEVELKKDRDFELDYWRNI
                     VKTASSPTDDPVASAWADTLTRYHIPHHYALQLIDGVARDLSQTRYQTFEDLATYCYG
                     VASTVGLMSMYIVGFKGHDAVPYAIKLGVALQMTNILRDVGEDYRNGRIYLPREELVF
                     YGIQEADIAEGKVTDRWRQFMKFQIDRTRQLYEESWEGVKMLEREGQLAIGAASTFYC
                     GILEDIEKHDYNVFNRRASLSAMGKISRIPSLWLKVRSL"
     gene            19088..20623
                     /gene="crtI"
                     /locus_tag="PL000_02030"
     CDS             19088..20623
                     /gene="crtI"
                     /locus_tag="PL000_02030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HNC87700.1"
                     /translation="MRKQTAVVIGAGIGGIATAARLAKNGYDVTVVEKNDTPGGRCNQ
                     IIQDGHRFDIGPTLFLMPEVWEETFAALGEKLSDHLDLRRIDPTYKVRFDDGLQLELT
                     SNIGDMQTQLEKVEKTAFTGFLNYIAEGSKHYKISLEKFVGRNFYNIFEYFSLSNLPL
                     IFQLKALGKHYTNTGRFFKDERLKAAFTFQNMYLGLSPYDAPATYSLLQYTELAEGVW
                     YPMGGMYAGIQALTRIAEKLGVKFIYDAPVKKIKTGKSKVDGVILEDGRELTADIFVG
                     NADLPYIYDQLLPDRAEAKKLIDEKLYTCSTIMFYWGVDKEYSQIAHHNVFLGGEYKA
                     SFEKIFNEHTLPEVPSFYVHAPKRTDPAAAPEGQDTLYVLVPVGHLDARSAQDWDALV
                     ARARRYVLDRLAKEMDAADLEAHIKFEIVYQPKTWKERFNLVNGSAFGLSHNFWQVGY
                     LRPHNRHKTYKNMYFAGASTHPGTGLPIVLLSARLTTERILKEFTGQQVEGYKLEKAV
                     AQG"
     gene            20701..22023
                     /locus_tag="PL000_02035"
     CDS             20701..22023
                     /locus_tag="PL000_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC87701.1"
                     /translation="MSISKNPAFNLKVVLQETGIAADTLRAWERRYGIPMPQRTAGGH
                     RLYSQYDIETIKWLMARQAEGLSISRAVDLWNEHNASGADPLAGFIPTTNIAPLATPA
                     ITLSADTSLDSMRAQWIDACLNFSEATAEQVLNQGFSMFPVEVICTEVLQRGMSEIGG
                     LWYENRASVQQEHFASGLAMRRLDALISASPAPSLKQTVLVGCPPNEWHTFTPLLLCL
                     LLRRRGFGVIYLGANVPAEQFEETVSSVHANMVVLVAQTLITAAALHSTALSLVNMKI
                     PVGFGGRIFNIHPALKEYIPGHYLGNTVTASVERIEQLLKGNQSKVQAKSATEAHHVA
                     LEHFNSQRLHIESSIKEMLPLFVAKDIDTGIHFLGDNIAAALQLGDMEHVTAELEWLK
                     VLMQSHQRPASELADFMHNYATAVDKHINGRGEPIKVWLNEQARLTQL"
     gene            22020..22592
                     /locus_tag="PL000_02040"
     CDS             22020..22592
                     /locus_tag="PL000_02040"
                     /inference="COORDINATES: protein motif:HMM:NF021577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HNC87702.1"
                     /translation="MTKQDILSTFYHKLLPLFPPRTEGYLNRLTAQDLQHSNFRYIFS
                     VQSMIEISHMIEDSVINTPGVAETHVSFQYFSRIPAQLEQYRKVSEASTGLWLYGVPD
                     APLPQLSRTIGVSTDGTPLETYWFVVAYGPGLYATLLAEEITPEEGQRMYEGFYTFEP
                     ETAFQVVSLLHQMFPMQVPAPIAPQDQEHA"
     gene            22594..23430
                     /locus_tag="PL000_02045"
     CDS             22594..23430
                     /locus_tag="PL000_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC87703.1"
                     /translation="MNIRIVTDSTCDLPDEVTKQQAITVIPLNINIGNDTFQDGIDLT
                     RTEFYARLPEYSPSPKTAAPGPDVFKKEYERLADEGARSILSIHISETLSATINSART
                     AAEQFTRIPVIVLDSSQLSLGTGFLVEKAAIMGAAGETMEMIVSTLQKMMKRTYVFAS
                     LKTLEYLRRSGRMNFALASFGELLQIKPLLHMHLGKATAHRTRTQKRATERLLSWLNE
                     YAPYEKLAVLHAGAQDEAEALREKVRSFLPNDDVSIIQITPVLGAHLGIGALGFACIS
                     KE"
     gene            23435..23761
                     /locus_tag="PL000_02050"
     CDS             23435..23761
                     /locus_tag="PL000_02050"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HNC87704.1"
                     /translation="MKIAKLISILGILAMTAALVNGFTRGDFFGEGGQLAAMPWGIVS
                     LVDLYTGFTLFSGWIIFREKSLPVAIAWTVAMMVLGFFAGSLYAFIALQTSGNDWRKF
                     WLGKHA"
     gene            23754..24452
                     /locus_tag="PL000_02055"
     CDS             23754..24452
                     /locus_tag="PL000_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HNC87705.1"
                     /translation="MPERTLAPQGKGRELLEELQTVFAGRNSFMDAILPPILFLAVNG
                     LVNFQAAMWSALALAIFFAAFRIVRKQSLMYALAGVGSVAAAIGIAWFLGKSEGFFLP
                     GLISGSVTLFLTILSLVIRRPMVAWTSYLARRWPLEWYWHPLVMPAYSEVTFAWAVFF
                     AIRLLYQFVFFQDADVNSLAALNFITGWPATVLLLVVSYLYGTWRLAQLRGPSVQEFL
                     DDSPAPWQGQRRGF"
     gene            24479..25345
                     /locus_tag="PL000_02060"
     CDS             24479..25345
                     /locus_tag="PL000_02060"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNC87706.1"
                     /translation="MKTTDRNALIAFPILILIGALVALAGSQGGASIAGVPLFAASVG
                     LAFLIQWLVFIPAYLMQTEKFFDLTGSLTYISVTIIAVIFSGTMDGRSVLLAALVVIW
                     AVRLGSFLFNRIRKAGKDDRFDEIKPSFIRFLNVWTIQGLWVTFTMAAALVAITTSTK
                     KGLDVFAIIGLVIWVFGFVFEVVADSQKSRFNANPANKGRFIQTGLWARSRHPNYFGE
                     IVLWLGIAVIAIPVLQGWQWVALISPLFVTLLLTRVSGVPLLEKKADQKWGGQEDYEA
                     YKKKTPVLIPRL"
     gene            25364..25711
                     /locus_tag="PL000_02065"
     CDS             25364..25711
                     /locus_tag="PL000_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HNC87707.1"
                     /translation="MIIQILQIIAAIATILTGLVSLFKPESVTGFTGLRPEGGRGITE
                     IRAVLGGFFVALGAAPLMLNSPVAYTMLGMAYLVVGVVRGVSMWVDKSVTQSNIISLV
                     VEIIFGVILVVPV"
     gene            25708..26190
                     /locus_tag="PL000_02070"
     CDS             25708..26190
                     /locus_tag="PL000_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:25837..25839,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HNC87708.1"
                     /translation="MSRAELNKPAPNFTLTDFNGNEISLTDFHLKKNVLLVFNRGFFU
                     PFCRRHMAQLRQDYNKFTEQDTVILVVGPENSLAFSLYWKTNSLPFIGLPDPSHTVLK
                     RYGQEVNLFKFGRMPAQVAIDKNGIARFVHYGHDARDIPTNEEILELLESMNKESITS
                     "
     gene            26200..27420
                     /locus_tag="PL000_02075"
     CDS             26200..27420
                     /locus_tag="PL000_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC87709.1"
                     /translation="MNNKRLFSIILVVFIDLLGFSLILPLLPYYAETFNANETITGIL
                     IASYAVMQLIGAPLLGRLSDRFGRRPVLLISVFGTFIGFLLLGFANALWMLFASRILD
                     GLTGGNLSVAQAYISDVTDEKSRSKGLGMVGAAFGLGFIIGPVTGGLLSQWGYAVPAF
                     VAAGISFLNLILIYAWLPESLTEEKRNQMTEKRPAVTLQALIVAFKRPFTGSLLITRF
                     FYGLAFSIFQTIFSLYALAKFDLTARDTGFVLTYVGVLAVIVQGFLVGRLTTQFREDL
                     LITVSVVLMGISLLGWALAPSVLWLYIIMTPTAISGGLLNTLLSSTLTKAVAPQEIGG
                     ILGLSAAVESATRIIAPLLGGVLLQQVGTWAPGAFGAVVMTGVSLYVFTTIYNHPIVA
                     TLKQGKTAPVPASD"
     gene            27456..28076
                     /locus_tag="PL000_02080"
     CDS             27456..28076
                     /locus_tag="PL000_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87710.1"
                     /translation="MTDKTTRYELPPSGITLLGYVVRRMHKTRWLLPVPDLLVQGKTD
                     MALFNVAMYAVASSTSFGRAYYQPQFNSAGAEITETDPLTGANVTAKLVATSPHYFIT
                     YTAAMPNGDLFTGSEEITGTTVGLRGLGMPAPSKFTFTSGGYTAELSGVLTSELALSL
                     IGRTRIRAYGFLNFKDNAGNTGKLNLDRSRKIKIDINDKAMEPKFI"
     gene            28073..29413
                     /locus_tag="PL000_02085"
     CDS             28073..29413
                     /locus_tag="PL000_02085"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC87711.1"
                     /translation="MTSIWWIRRDLRLTDNLALHSALKTGDVIPVFIIDPAFSSQSPR
                     RRSFLYEGLHTLDQDLKKRGSYLVLRTGRPVDVLHQLMQETKAQAVFAEEDFTPYAHK
                     RDTHVAQSVPLHLLPGQTAQHPSRVHKQDGKPYTVYTPYSKTWKAMLEEIELVPAPDV
                     ISTPAGISTEPIPAFGVNPLFPAGEQEALTRLDEFAFKRIHAYDEARNRMDLDGTSSL
                     SPYLRFGMLGLRQAVRLAKQASAESRGAEIWLNELIWREFYIQILYHFPYVSHREFNP
                     ALMGIRWRNNIEEFECWKEGRTGVPIVDAAMRQLKETGWMHNRARMIVASFLVKDLLI
                     DWRWGEAWFMECLLDGDPAANNGGWQWTAGTGTDAAPYFRIFNPVLQSAKFDPLGEYI
                     RKWVHELRGLNEKEIHAPWEHGVKISGYPEKPIVDRALVKERTLQAYKQVKSER"
     gene            29469..30722
                     /locus_tag="PL000_02090"
     CDS             29469..30722
                     /locus_tag="PL000_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC87712.1"
                     /translation="MNSKITPELGRAAMRALFETRSPLGPLKVMAQHVGRFFQIPLPG
                     FRPYVVFGPEAARKVLITERDKVLWRNTDPVTDLLGRGVLITDGKEHDHYRELMEPPL
                     HPSNLSGYTQMMLEKTDQVTSHWKSGDTVDMLVESRKIALLIIMQTLFSKDVWADLPR
                     IWKPILKAIDFISPGAWIIWRNMPRFGYRKPLRELDDYLFEIIEERRKTKQAPHDLLQ
                     HLIDAGLTDKVIRDQMLTMMIAGHDTSTALLAWTFALLGQHPETLKHVLAEVHSKDRS
                     PLLDHVIKESLRLYPPIHIGNRRVAKTMEFAEGTVPAGERMFYSIYLTHRDPSIWENA
                     EEFQPERFAHGRKTPPMSYIPFGGGPRACIGAAFGQAEARIVLTRLLQTFSFEPMNPD
                     KIHAHMGATLEPRPGVLMKVQPLIP"
     gene            30719..31510
                     /locus_tag="PL000_02095"
     CDS             30719..31510
                     /locus_tag="PL000_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HNC87713.1"
                     /translation="MTYFGFLLRFLVIPILIFLAITWWDNKNNRPTLGFTNGRAVWIA
                     ILVHILLAVTYTTPWDNYLVATGVWFYNPNLVTGIVFGYVPIEEYTFFVLETILSGLW
                     WWFLARRISEPGGFKASMPARVTSSIVLFIAWVIFTAIFFSNNEPLTYLSITLFWAFP
                     AILPQLLYGADILWHHRTLVLLGILIPGTYLSLMDIIALNETTWSISKAQTTGILFFG
                     ILPLEEVVFFFITNILITFGMTLLLSNIGRQRFSDWKLNNYKGLP"
     gene            31507..31887
                     /locus_tag="PL000_02100"
     CDS             31507..31887
                     /locus_tag="PL000_02100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNC87714.1"
                     /translation="MKLEQFKKQQFLNIESFRKNGQAVKTPVWFVQDGETLLVWTQAG
                     SGKAKRIRRDGKIRIAPCTGSGELLGEWAPAYAKTNESVETVKHTTTLMKKKYGLFFT
                     IFGFIGRMRGGSNYTTISIQLQNQ"
     gene            complement(31969..33324)
                     /locus_tag="PL000_02105"
     CDS             complement(31969..33324)
                     /locus_tag="PL000_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HNC87715.1"
                     /translation="MPQTTIACPRCKQMIPANVEQLFDVTHEPAAKQRLLSGQSNHAR
                     CQYCGYEGRLATPVVYHDNDKELLLTFFPFELGLPVNEQEKLIGPLIKQVTERLPLEK
                     RKAYLLKPQANLTYESMIETILNKDGISSEMIKTQQERVMLIEKLIRMTSADARLEMI
                     RQNENAIDEQFFSLFSRIAQNAMQSGQETIARALIDLQRQLLDETAFGRQLRDSVAEL
                     EAAQKTLQDAGQGLTREKLLDFVLESQNDSRIRAYVSLARGGMDYVFFQLLTEKIEKA
                     AGEEKSRMEGIRDKVLGFVSDVDKQMEARYKQAQDFVENLLAQEDIERATQENLENFT
                     QDAVEVVNQLLRQASEKNDYARMGKLQKMVQVLQAASTPPEVAFIEHLLQAPDEATLD
                     AMLSQNEEMVNQQFIDALSGLVAQMDSQGADNPEAKAMGEKLTEIYRVALKRVMKKNM
                     G"
     gene            complement(33428..35236)
                     /gene="pepF"
                     /locus_tag="PL000_02110"
     CDS             complement(33428..35236)
                     /gene="pepF"
                     /locus_tag="PL000_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNC87716.1"
                     /translation="MATSIPPRSKIDKKYKWNSESVFPSDKAWEKEVEQIIADIPEVK
                     KHQGGLGESAAALLAGFQAFEAILIRAQKAYMYASFSYSVNTTDQTAAGMRAKAGGMY
                     GQVASAVSFLQPELLEIGKDKLDQFFQQEEKLGVYKFYVENLFRKQAHVRSAEVEEVL
                     GMVSEPLGGFYNSTSMLTNADFKFKSIKDSAGKTFDVIQGNYDTQLMGLADRKARKAA
                     YITYMDQYVAHKNTLASNLAHNIQSNVFNMRVRKHESTLSASLFDLNISTDVFHNLID
                     TFKKNIPVWHRYFNIRRKALGLKKFAYYDIWAPIEKKKVKIPYDKAVDMICEGLAPLG
                     KEYTAQVRRACTTERWVDVYPNQGKGHGAFSSGSYGTHPFIMMSYTDEMVSMSTLAHE
                     LGHSMHTYYTFKNQQFINTDYSLFVAEVASNFNQALLRGHLLNTVKDKNFQVALILEA
                     VGSNMFRYFFQMPTLARFELETHQRAERGEPLTADSMIELMADLFAEGFGPHFDMDRE
                     RVGMTWATFPHLFADYYVYAYATGISGAHALAGRILRKEPNAVEDYLGFLKAGNSDYS
                     LNVLKNAGVDLTTPKPVEETFAVMEGYINRLEELLG"
     gene            complement(35303..36868)
                     /locus_tag="PL000_02115"
     CDS             complement(35303..36868)
                     /locus_tag="PL000_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87717.1"
                     /translation="MSIIPSMLPDFRVRQRDYLLEISRLITEELDLEKLLARILRVSI
                     EMLAGQAGLIALKQADGWRVSAAHGIAPAFLSYLTPLLAEEKVRELDVRELNRMLKNL
                     TYTASMGLLNGTGLPLAAHGQVIGVIFIFRNYADLFTPDDRVLLQSFADQAAIAVYNA
                     QLYGQVSYEKKRLDALLESAADGILILDANLDIERCNGAFEKLFGQPREEITGKNHAD
                     IIKWKKTPQGITLEDSVAEGWPLTPNATLYVEGDLAKPAPPPLPVGITYAPLLSEEGK
                     LRNVILSVRDITHFRTADEIKATFISIVSHELRTPVALIKGYASTLRRDDARWDKHTI
                     NDSLAVIEEEADRLSKMVDDLLDASRLQAGGLSLNQADVAVPSLALRVSERFASQSPK
                     HSIVTDFPSNFPVILGDENRLEQVISNLVSNSLKYAPKGEIKISGSVLPEQVIVCVSD
                     EGPGIEAKDLPHIFDRFYRSTNAVKQTKGAGLGLYLARAIVEAHGGHIWADAVTGSAK
                     SESGARICFSLPR"
     gene            37095..>41074
                     /locus_tag="PL000_02120"
     CDS             37095..>41074
                     /locus_tag="PL000_02120"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HNC87718.1"
                     /translation="MRIFSIQAFNQSKSAMVRLFVFVLLLASMLAQEHVPGVQAQAGT
                     PQLILTKTIEGGATTASLGDTIRYRIRFECSSLTTSCGQMEITDVLPAGLTYAPPPAS
                     SAPTGFTITEAPAGTITITKDDNNLLDGTQYDVVIAATIDYDLRPLPATINNTVNGRV
                     DPPGAVSWQNATPASAPPITVSNVGEYWDLTKTLYSPSVNPTINTDVTYRIQLCPTTP
                     PPGTSNVALENITITDTLPTGATFVSASDAGTEAAGVVTWPLIAGPVFPPTCVTRYVT
                     IRYNSPTFAVGNNVTNTANASGTYTDSGGGTIGPISIATDPITHPIATVLEVPTYSKN
                     DQGDPVGITGTARFVLTLNTNGTNSPANDLILIDTLPPELQVTSVTSGQWNAAFNYVR
                     AYVEYSTNYGSSYTAFPGQPISYNSNATYTAPAANITNVRWRFEYDPDAAAPFLFTQA
                     GLPFAWAFTTSPQIRVTPRATATTADAPSGVAMPAAVAGTRYENCLQTSRTSNAGAPV
                     TDPCDIEGMTVQGTLASLRASKAETRGTSWDADNDPLINTFTSDSTLLPGDTLRYTIT
                     VSVTERSSQAMIDPTILDTIPADLIFVRNGTASVNGTGCPALASQPTFTQAGTQLKWD
                     FTGFSLDHQPSGLGSCNLVVEFYARIPAGQTPGTRTNDLILVTDSDDIVCENGTEIVD
                     TTDIDSDANNTEPACQNPDTYVVERSAALRGEKWIRSVDPENSVVVDSTTFQPSASCP
                     DGGTVGLAGGGSNPFTRFPCISQAFPEGALSIGQRVPPPLNSALDDFEYNLRIYNDGN
                     VEMLSYALYDIFPYYGDTGSGGTLANSPRLSEFRPILRGPVEFISGPSLSSTDFTIEY
                     NNTTNPCRPEVFNQPAGTPNIPAGCNNTWSSTWSADARSFRILLNSPAVIPPSATTSE
                     VRFGIPMYIPKDAPTVGVFDNNDPLSLEIAWNSFSHVGSYDSDPNVPVVIQDLLASEP
                     RKVGITIPERMSVGNRVWRDSDNSGHIDAPDDIAPGIAGVTVNLYRDVDDDGVPDGPA
                     IATTTTDSLGYYLFSNIPHDLADVNNNRYLIGIPSSNFGSGQPLFNLRSSTGVFATTD
                     DSDDNGIDPVTAGQEVFSASFILQPTTEVTGETDLSSNDLDGPPGERRGTNAERDYNS
                     DLAIDFGFFGGTDVPFSIGNHVWFDNGRTGASTFNYALNNDGIRQATELPVVGLDIRL
                     YRDGNGNNTPEPAEMIRTDITDANGFYLFDNLDPGPYIVEIPANEFGAGQPLEGWNNS
                     QVIGTETTGVPGNTNIPNVDSDDNGVDSSTPHVDGIFSGVIVLTRGTNETTGETHISN
                     QPDPDG"
     assembly_gap    41075..41174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <41175..45103
                     /locus_tag="PL000_02125"
     CDS             <41175..45103
                     /locus_tag="PL000_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HNC87719.1"
                     /translation="NETTGETHISNQPDPDGNAATPNLGINPTEWDGPASRGRYGESD
                     TTSNLTIDFGFFEVYSLGNVVWVDDGTGGGTADDGLRNGTEPGLNGVTVNLYLDANND
                     GRPDTVTPKATTTTANGGYYRFDNLAADTYLVEIVLPAGYSSSPIDAGDPDTDEDDFD
                     DNGVVPATISTVLSNPVTLGRGTDEPTVDSDAATANNPEPGEAPNHHSNRTVDFALTL
                     GYSLGNRVWVDLDDSGTINAADGATPGLDNMTVNLYRSSDPSTIIATTLTAGGGYYRF
                     DNLLAGDYIVEVVSPVGYTSSSVDANDPNTDNDSDDNGVIISATTVRSNPVSLGGLEP
                     STDDDPLPNPAATGEAPNTRSNRTVDFGFFFVPYSLGNRVWDDSDGAGGTPDNGVRDG
                     AEPGIDGVTVNLYRDSDNNNTPDGAIIATDTTSGGGFYRFDNLAADTYIVEVIPPAGY
                     ASTLDAGDPDTDLDDDDDNGVVFAGANIRSNPVRLGPPEPSEPTLENNPAVNPETADG
                     EAADDHSNRTVDFGFVLGYSLGNRVWDDSNGAGGTPENGLRDGTEPGMDGVTVNLYRD
                     SNDDSNPDGGIIMSTSTSGGGFYRFDNLMADTYIVEVVIPTGYTSTLDAGDPDTDIDD
                     DDNGVVIVGSNVRANPVTLGPAAVPEPTLENNPATNPVAGESPDARSNRTVDFGFIRS
                     STPPPPTGSGIQIVKTASASVVKPGDTVVYSYVVSNTGTTPLRNVIVTDDKCSPVTYI
                     SGDVNANNILETTEKWNYTCSAILSETTTNIATVTGKDPTGGDVKDTDTVTVTVDTNG
                     FSKIITGTSETFTNGMDVAIGEIVTYKITMSLPAGLPYNNVVVTDRMDKGLAFVDCVS
                     ATLGGVDILNQICPPLVSAIKDPIDPASNPANPGRQAQFNIGNVSASTKPTALVITYR
                     AIVLDVIENQEKDTLNNKATLTWDGGSLTASAPEVVILEPDLDIEKAANPTTVTAGSS
                     VEFTIFITHTSLSLTDAFDVVVTDVLPKGLEYVACTPITYEGVVPTKQPDPCSIPNGD
                     TLQFEWDVLPLGGNSVIKFFARYDGSQVSVMNSAAVAWTSLPIDYINGKPVQHSGYND
                     TSTERWYDPNDLVNIYQISDNVWINPPTITEPIEEEELPGSLPATGFAPNVITALPQQ
                     PANKAYTTTDIWVEIPSIGVNMPIVGVPLVDGDWDLSWLWNEAGWLNGTAFPGWDGNS
                     ALTGHITLPNGRPGPFAMLGNLKWGDKIIVHAYGTAYTYEVRETRTVSPDNMTILKHE
                     EESWLTLITCKTYDKATNTYARRTAVRAVLISTTADKQTKEAKSGR"
     gene            complement(45175..46497)
                     /locus_tag="PL000_02130"
     CDS             complement(45175..46497)
                     /locus_tag="PL000_02130"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HNC87720.1"
                     /translation="MTNPNLPGPKAKAMIERDRKVISPSYPRGYPFVMDHGKGTEVWD
                     VDGNRFLDFMGGIAVVSTGYSHPKVVKAIQEQAEKFIHISSDFYHENWIRLGEKLSDI
                     APFKEDALTFMTNSGTEAVEAAIKLARYHTKRTNFIGFTGAFHGRTMGAVTFTASKAK
                     YHGGFYPLMNGVTHAPFPNPYRPVLERRKGEDYGEAVVRYIEEEILAQILPPKDVAGI
                     LVETIQGEGGYIIPPEGFYPALRKLCDKHGILMILDEVQSGMGRTGKWWAIEHFGVEP
                     DMITSAKGIASGMPLGACIARKSVMDWEIGTHGNTYGGNPISCAASLATIDLIENGFM
                     KNAEEVGSYALDALEEIKSRHPSIGDVRGKGLMIGVEFVKDRETKEPDHDLTERVVDL
                     GFERGLLMLSCGKSVIRIAPPLSISKSEMEEGLKIFEDALTVAEKEMK"
     gene            46917..48239
                     /locus_tag="PL000_02135"
     CDS             46917..48239
                     /locus_tag="PL000_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNC87721.1"
                     /translation="MRKNFLAEFWQALRDIPRWLRPGLGIKRWLTLVLIGITLLGLGL
                     AVILIDLYRTDSENPILLTFLSYASLRFIPRVWRAVIFGGLGVGVVIYGIVQLNRSLL
                     TPFVRPGADVYGQLTDFRRRERGPRIVTIGGGHGLATLLRGLKTHTRNLTAVVTVADD
                     GGSSGRLRESHGILPPGDIRNCLAALSNDEAMLTQLFQYRFSGSPGLEGHSFGNLFIT
                     AMADITGSFEEAVAESGRVLSVNGRVLPSTLHDVKLVASMELPHSLNEIRVEGESKIP
                     TMAGRVRRVWLEPDNAPAFPPVIKSILNADVIVVGPGSLFTSILPNLLVNDLLAAMHA
                     SRAIKIYVCNIATQTGETDSYTCFDHVRALEGHVGEDLFDVILCNDNYRGIISADSKW
                     VLADEKILSDSRTYCADLVDDDHPWRHDSEKLAREIIQIFSEKTGPLV"
     gene            48426..49484
                     /gene="gap"
                     /locus_tag="PL000_02140"
     CDS             48426..49484
                     /gene="gap"
                     /locus_tag="PL000_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNC87722.1"
                     /translation="MTVKIGINGFGRIGRQVLKAIRDMYPNELEVVAFNDIGDTKTMA
                     HLLKYDSTYGRFNGSVEIADDGLLIDGKKVKVFKETDPAAIKWSELGIDIVIESTGLF
                     TIKDDGVNKKGKTVKGAVNHITSGGAKKVIISAPAEGEDLTVVLGVNEDKYDPAKHHV
                     VSNASCTTNCLAPAAKVVHDKLNIKRAFMTTVHSYTNDQKVLDMPHSDLRRARASGLN
                     IIPTTTGAAKAVALVIPDLKGKFDGYSLRVPTPTVSVVDFTAEVEKETTTEELRQMFR
                     DAAKEPRFKGILEAVDEPLVSMDFKGSSYSSSVDLPFTSVLGKEKNNFIKVVTWYDNE
                     WGYSCRTADLAAMMAKTL"
     gene            49532..50704
                     /locus_tag="PL000_02145"
     CDS             49532..50704
                     /locus_tag="PL000_02145"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNC87723.1"
                     /translation="MNKKTVKDIDLKNKRVLMRVDFNVPMADGKVTDDKRIKAALPTI
                     KYVLEQGASLLLMSHLGRPKGGPDPEFSLRAASEALSALLEKPVQMAPDCVGADVEAM
                     AKGLKSGDVLMLENTRFHKGEEKNDLDLAKQMAALGDVYVNDAFGSAHRAHSSTEGIA
                     RFLPAVSGFLMEQELEYLGRAVANPEHPYIAILGGAKISDKILVVETLASKCDKLIIG
                     GGMANTFLAAKGLNMQDSLVEAESLETAKSLLGKLGEKLVLPVDAVIADKFDAEANTQ
                     VVDVDKIPAGWRMLDVGPKTISLYQATLNGSKLIVWNGPVGVFEMPKFAEGTFALARM
                     LAESKATTVIGGGDSASAVKKAGVAKQMTHVSTGGGASLEFLEGKELPGVAALLDK"
     gene            50882..52732
                     /locus_tag="PL000_02150"
     CDS             50882..52732
                     /locus_tag="PL000_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87724.1"
                     /translation="MSFNIGENVGPYRILEQLGQGGMATVFKAYHASLDRYVALKVLH
                     PAFNQEQSFISRFQREARVVAKLEHPNIVPIYDYSEHEARPYLVMKYIEGDTLKARLN
                     KGPLSAGEIETVVNAIGAALAYAHRQGILHRDVKPSNVMLATDGLIYLADFGLARIAQ
                     AGESTMSADSIMGTPQYISPEQAMGKKDLDSGTDIYSFGVMLYEMVVGQVPFSADTPF
                     SIIHDHIYTPLPLPRKINPNVPESVQRVLLKALAKDRIDRYETIEELVQAFKDAWTEA
                     GVPMQGTSITMHSTNLRKQVQSTASKPKPAMPNLVNEKPRPQAKAPAKKGSAVWMWLG
                     VALTGFICIIIAFIAIRNNNRIAPLPVQPTLVPTAVILEPSPAIPTQSAPPTFPPLTP
                     PLGTDTTGQPPLNPQGSDTPEIAAAREVVAKNPQDADAHLQLAFALWDAKEIRPSMDS
                     LMQAMGLAGPGNTDFLQKAASGFHQREAWVAATAIYLRLQASYAGAPVPEDIKINTNE
                     SAYKAADRKDFLLLIAFDQIDAIDPRLGHVVRGRQALFNNNLQEAQLQLAEAEKLEPK
                     IPEMFLLKAEIALRNGNTSEARTIFNNTLNNPATPAWMRLMIEELLKTMQ"
     gene            52743..53510
                     /gene="tpiA"
                     /locus_tag="PL000_02155"
     CDS             52743..53510
                     /gene="tpiA"
                     /locus_tag="PL000_02155"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC87725.1"
                     /translation="MRKPLVAGNWKMNKTITETRDLVFTMSMKLREIDGVEKVICPPF
                     MSLMAAAALLEGSGIGLGAQNMHWEEKGAFTGEVSPAMVKELCGYVILGHSERRSYFG
                     ETDQIVNRKLLAAQAMDLVPIVCVGETLEQYESKLTREVVSTQTSQSLRGVSPSYAPR
                     MIVAYEPVWAIGTGKASNGPDANDVVKDVIRPALADLFGNETAQAIRVLYGGSVTAAN
                     AAEFFGQPDIDGALVGGASLKIDEFVAIAKAAASTAA"
     gene            53545..54300
                     /locus_tag="PL000_02160"
     CDS             53545..54300
                     /locus_tag="PL000_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87726.1"
                     /translation="MPFTGFLLFILLLLPLVFLQRLLHREIQAVLLILTRNSQLTIGL
                     FSVLFFPGVFLHELSHFLMAKLLQVRTGNISLIPAALPNGRLQMGYVETEETDVVRDS
                     LIGLAPLIAGSLFIAYAGLNRLGFGMLMDLLANGQFALFWTALGYLPQVDDFYLWFYL
                     VFAVSSMMLPSESDRHAWLPLGVWIAGLFALAIFAGAGQWMLENLAPSLNDFLGTVAT
                     LFGLSVAVHVILLLPTMLLHRALSRITGLDIKK"
     gene            complement(54556..55884)
                     /locus_tag="PL000_02165"
     CDS             complement(54556..55884)
                     /locus_tag="PL000_02165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNC87727.1"
                     /translation="MCDTLIASKQATSDNIAIFAKNSDRPPNEGQHIVYFPAQSHAAG
                     SSLKCTYIEIPQVEKTHAVLLSKPFWMWGAEMGVNEHGLVIGNEAVYSKVPANKKPAL
                     LGMDMLRAALERAITPREAVQVIIDLLEKFGQGGNAASHGETMYYHNSFLIADANETW
                     LLETVDKQWAAKQITDVYSISNCFTLGGQFDIASKELVENAVQKGWVKSGSQFDYAEN
                     YSDFIYTNFGKGRVRRATTTSLLETQKGSVTLETMMGILRHHDDEHYDPQNTVAAMDV
                     CMHAGFGPIRISQSTASLVVLLDDKTPLIYVTGTSAPCTSIFKPMWMDTASSLNLGPA
                     PASKYDSASTYWKHEQLHRATLLNYSERIKTYASDRDAMEKKFLSESRRLANATVKER
                     AEFSAHCFAEAAQAEAEWLKRAESIPSQKRALHSLAWNTFNKKAEMPPIR"
     gene            complement(55944..57884)
                     /locus_tag="PL000_02170"
     CDS             complement(55944..57884)
                     /locus_tag="PL000_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87728.1"
                     /translation="MDEKTVPLSRLPVDVKMHHAGRNLLGTDLDYRALFEQTGECVFI
                     ISLDFKIVTANLQALKLLAYEEHQLVGLPVEAIMLIDDSQTRETLLERRTNPSEYTLR
                     KRDGSMLPVELSISMVHNEHNMPTYIQMLARDISEHKQAEANLKRHMLSLSVIGEASA
                     GLFRSSNIETKIPEVLESLGYAVDVFCCALFDIMANSMKVQFQWKSYDASTFDLTSTL
                     EPFLASISENPERVFSVTNVKLQNQEVPTVSILVIPVQGTLGSRGFLALFDKETELSW
                     LTAEFDTVQTTVNLIGAALERLHYEKTIRLSEMRNRILVDALPDLLLRTDLTGHILDY
                     NAHPSHPLYMPRDLVVGKRLDQLWPADVVNMIIETEDQDAFLSSHWVYGFNLPNHQQT
                     FEARLHPISAEEALIIVRDISEQARLDQMKSDFINRASHELRTPLTAAIMMTELLQQG
                     GTEEQMSEYWHTLTSELNRQKNLINELLMAGRLESGRMKLDAVPMELAPILQESVRAV
                     KSIARMRDISIELSVDSDICSVLGDAGGLSQVFINLINNAVKFSPEGSTVDVAAVCGG
                     AEHEARISITDHGLGIPPEAVPHLFERFYRAKNVTVAEIPGTGIGLYIVKSIVDELGG
                     KIDVISELNQGTTFVVHLRLAP"
     gene            complement(57877..59013)
                     /locus_tag="PL000_02175"
     CDS             complement(57877..59013)
                     /locus_tag="PL000_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87729.1"
                     /translation="MENKPIILVVDDEPAIRMGLAATIERHGYGVITAMNGSDAIVKA
                     KKSPPALIVSDVMMPVVNGFDMKKQMSTDPQLASIPFIFLTARTASEDRVAGIRGGAD
                     DYVTKPFVTEELMARIDAVLRRVQTEQARGREQVQHAAQQEMDKLRREVLQNFRHELR
                     TPLSNVIMSLEMAVNTKYKNPDEQAEFIRIALASVDRLDTLVADIILLSDMDQHDLNP
                     TRQLIDVEHHILSPVQKRLHRYPLKRINFVYDIKLANPIKAPRKEFSQALAHLADNAF
                     KFSPEHGQVSLKVRSGENGGGTITIQDESVGVPVELREKVFERFYQISQGDQREFQGL
                     GVGLTVARAVFSNLGGEVKIVDSEKGCCVVATLPDMRPEDVEYG"
     gene            59197..60459
                     /gene="obgE"
                     /locus_tag="PL000_02180"
     CDS             59197..60459
                     /gene="obgE"
                     /locus_tag="PL000_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNC87730.1"
                     /translation="MFIDHVTIHVKSGKGGDGMVHFRREKFVPLGGPDGGDGGRGGNV
                     IFEVKATLNTLSAFRPNEKFAAEPGKNGGGSQMTGRGGKDLIIYVPPGTVLYDAETGA
                     LLGDLTTPGQQLLVCKGGRGGLGNQHYATSRNQAPRTAERGEPHEEKMIRLELKLIAD
                     IGIIGLPNAGKSTLLAALTNAKPKIGDYPFTTLEPNLGVAKIDDDTTVVLADIPGLIE
                     GAAEGAGLGHDFLRHIQRTRVLIHMVDGQSEDPLADYTQINNELALFDPNLAKKPQIV
                     ALNKTDQPDVQERLKSIKASFKKKKIELFTLSAMARTNTRDLLIAAHQRLADMPVEEV
                     EETLPVYKPDVDPNHFEITREDDGSWRITGVAIERSAKMTYFEHDGSLRRFQKLLQRL
                     GVDDALREAGVTDGDTVFIGNFELEWKE"
     gene            60475..60882
                     /locus_tag="PL000_02185"
     CDS             60475..60882
                     /locus_tag="PL000_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87731.1"
                     /translation="MAQLSPNKQRALTIGMIVLGVIVAAFFGMRAFRAFNRFNGHRPP
                     PQGKVETDVELIRGWMTVPFISQMYRVPDRIIFEELNIPEKGNMEKSLKDLNQEYYPN
                     EDGFVIEMVKQIVQANLPNEPHDPPEPPTPPNP"
     gene            60892..61500
                     /locus_tag="PL000_02190"
     CDS             60892..61500
                     /locus_tag="PL000_02190"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC87732.1"
                     /translation="MSDFFLTQVINFGTPLVGLILFLGALGFPVGASVVVIAAGAFGQ
                     QGILDIPSAAIYGLLGAVIGDTLSFGMGYYAKDAVQRRFGNSAAWQGATETFQKNAGM
                     AVFLTRWLITAIAIPTNLIAGGSGYKYSRFISYDLTGEIVWIILYGGLGYLFGSQWEL
                     VYDFMSNFGGLILGIVLMGFGIRQALQWQKNSRDKINQKAEA"
     gene            complement(61525..62016)
                     /locus_tag="PL000_02195"
     CDS             complement(61525..62016)
                     /locus_tag="PL000_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC87733.1"
                     /translation="MSKDTVQDGLVVSMDYKLTVDGEVLDSSDEAGPLQFLVGYGNII
                     PGLEKEMMGMKIGDSKDVVVQPEDGYGEFDDDAFMDVPRTEFPADMKLEEGMELHVTD
                     EDGNHQAAYVASFDKDTVRLDFNHPLAGAELSFYVKVVALREPTEEELDHGHVHEDGH
                     HHH"
     gene            62155..63036
                     /locus_tag="PL000_02200"
     CDS             62155..63036
                     /locus_tag="PL000_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87734.1"
                     /translation="MRYDLCLPWYWEYDDAFVGMVERTCIEQGLSLWQIKPDNLLESI
                     TALYKGVTTFKTLLHRGQGEAIFDPIPRWAKEFNARRINPAEVSAWSEDKATMHLELI
                     SAGLHTPHTILLPPFLEQPVVSEINLAPLGGNFVIKPSNGGGGEGVIIGANTIEEILH
                     ARMEYPEQKYLLQAHITPRVIEDTPAWFRVFYANQETYPCWWNTETHAYRPVSAEEES
                     QHRLGRLRDVTKRIASICKLDWFSTEIALAEEFIVVDYVNDEIDTRVQSQALDGVPDE
                     IMECVAKQLVKIAKENA"
     gene            63033..64112
                     /locus_tag="PL000_02205"
     CDS             63033..64112
                     /locus_tag="PL000_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNC87735.1"
                     /translation="MKNVRARDLGIPFEGLTGENNAITDVSNVTVGYATVIEGPSART
                     GVTIIHPRGDKDHTPVFAGIHSFNGNGEMTGAAWVEEGGMLEGPIGITNTHSVGIVRD
                     SIVAWQVKNDALYQKWSCPVVAETADGWLNDMNGFHVNENHVLQALNSAKNGSIEEGN
                     VGGGTGMLCYEFKGGTGTSSRKLPAKLGGWTVGVLVQANFGRRYQLTIAGTPMGQYFK
                     DDAPFTNGENPFRQDDGSLIVIVATDAPVLPHQLKRLAKRATIGMARTGSLGGNGSGD
                     IFLAFSTANPQAAHDGEMGVANIQSLSNNYIDPILAATAYATEEAIINAMVAAKDMKG
                     HNDVDAKALPHDGVREVLKRYNRIG"
     gene            complement(64227..64751)
                     /locus_tag="PL000_02210"
     CDS             complement(64227..64751)
                     /locus_tag="PL000_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC87736.1"
                     /translation="MPKNIVLTLTGRDRIGLVDHVTSLVVKRGGNVGASRMAHLGGEF
                     AMLMLIALPDNEAGNIDQDFQSLRGEGYQVSIVQTEDSKKYEGWLPYEIEVTGADHEG
                     IIHDIAHYLASRGINIENMDTSSTPAPMSGTPLFTMKGVVLAPPKLNFHAWSDELEEI
                     GDKLNVNVIVGLVK"
BASE COUNT        15984 a        17945 c        15419 g        15289 t
ORIGIN      
        1 cccgtcggga ccgccttatt gaaggcaaat gctgtattca cagcaacaaa aagaccacat
       61 taaaatgtgg tctttttgtt atccatttat tgcttatgtc gtactgcttc gttctgcaag
      121 caactcttgc aaacgagggt ggttttcata atacccacga taactttcgg gcaactctac
      181 cgcaatacgg cacataattt catctgttgc ttcgcgcatg gcctgttcgc ggccactgcc
      241 tttggggatt tcaatgaaaa acgggtcgcc agcaactgct tttatttttg aacggcgaaa
      301 ccttttcaaa tttgcaacca cgccgccatc ttctgtacct gtcaaagcta caggtatgac
      361 gggatatccg cttttactgg caaggaaggc cacacccaat ttaccttgct gcatggatgc
      421 agtctttgac cgtgtgcctt ctggggcaat caccatcaag ccaccctttt gcatacgcgt
      481 caaaatctcg cgcaaggctg caaaatcagc ttcaaaacga ttaagccaga tggcattcgc
      541 tgcacgtcca atcgccccaa aaattggatg atccctgtat ttctcagcca ccgccatgat
      601 gatatcctca cgatcaagga tatacaacaa gacagccgta tccaggcggc ctaaatgatt
      661 cgcggcaatc ataacattcc cttgtggcag tttttcaatc ccgaccactt ccacatcggc
      721 aatgatgttc ataacaaatc gaacaatcga acgaagggtt ctaaatttca tgggtttatt
      781 taccagacaa caattctttc aatttgggat gctcggcata atagccgcgg ttcttttcag
      841 gcaacattac cgcaatacga cacatgatct cgtcaacata ttcctgtaag gtttcatcac
      901 ggttttcttt cggaaaagac ggcagggtga atggcttgcc tgcggtcagg tgaatctctg
      961 ttcggcgaaa ccgcttcagg ttggataaaa caattcgatc ttccgttcca gcaatggcaa
     1021 ctggaacaat ctgccatcct tttttagaag caagataagc gaccccgggc ctgcctcgtg
     1081 ccatcttttc atcgcgggca cgggtcccct ccggggcaat gaccagggtc tgaccatctt
     1141 ccattcgtct ccaaatttca cgcatcgcct tcaagtcagg attgaagcga tcaatgaaaa
     1201 taaagttcaa atgcctgcct aaaaatctcc agagtggatt ttcctcccat ttttcagcaa
     1261 cagggatgaa taaattccag ttatccaaag cataatacgc catgggagca tctaaaaagc
     1321 cgagatgatt tacggcaatc acaaaaccgc ccttttcggg gagattttcg tacccactga
     1381 tcttgatggt cgtaaaggaa ttcaaaacaa aagggatcaa ccagcgcaga aaacttctca
     1441 tttgttaatt ttacacgctt tccctgtctc acggcacggc ataaaaaaag aagtcctgtt
     1501 tgcacagggc ttctttgaaa gtactaaaaa tttactccgg cgggaatttt agctcacccg
     1561 tctttggggg cgttttatcg ccatacactt catacatctt catataaatt tcgcggtcta
     1621 caggggtggc atcaacaagt ttgaaaccaa tattgaacat attggggtcg aactgatcct
     1681 gcctgcacca catagggcgg gccttcagaa gaatataaga cttgcttgaa atttcgccaa
     1741 gctggtctat atacaggcgc atatcaatat taagtgaaac gctttgggag ctatcgatgc
     1801 gaaatccacc cgtgcttata tctgagatat gccccagcac tttccccgaa gcctcttctt
     1861 tgacgcgcat gtaataatta aaatttcttc gtgccaaccg tcgttgttcc gattccattg
     1921 gagcctcctg ggtactaata aaaaacgaca agttacattt cagtatatac ccatgaaaga
     1981 cctaaagcaa tcaaacttaa gtatcgtttc tcaagatgtg cgtgagaatg gatgcacccg
     2041 agccgccgat gttttgcgcc ataccgatct tcgcatcagc cacctgggtc tgaccacatt
     2101 caccacgtaa ttgctgaacc acctcaacaa gttgatagac acctgttgcg ccgacgggat
     2161 gtccacgggc tttcaatccg ccacgagtgc agacgggcac tctccccttg gggctgatct
     2221 cgttttcaag agcaagcctc accccttggc ctctttctgc aaatccgctg gcctccaatg
     2281 aaagtgcggc catgatcgaa aatgcatcat gcagttcaaa tacatctata tccgcggggg
     2341 tcacacctgc catttcatac gcacgcctgg acgattcgta agctgcctgc agaaataacg
     2401 ggtccttgcg tgagtgaacg gcaatggaat ctgttgctgc agcagaagct tcaacgacaa
     2461 tgcgcggctt tcttgcaact ttttcggcgg ggacaatcac catggcagcc gccccatcac
     2521 cgatgggaga ggcatccagc aaattaatgg gagtggacac catggaggat ttctcgaact
     2581 tttccacgga tattttttcg tgcaagcggg catatgggtt gtgcatggca tttgcatggg
     2641 cattgatcga aaactgtgca aagtcttcat gcttccagcc gtattcatgc atgtagcggc
     2701 gcatgaccag cgcgttgatc cctacaaagg atacaccctg ctccacttca taatcagcgt
     2761 cggcggcagt ggcaagggcg gctgtgacat ctcggccagc cttgtctgtc attttttcga
     2821 cacccactac cagcgctgat tcgatgtcgc cggaggcaac agccatcagg gcggcacgaa
     2881 acgcggcggc acccgatccg caggcggctt ctattttcac cgcttcctgt ttccacagac
     2941 cgatccaatc cgcaaggaag gagccgagtt gattctgccc attgatcagg ggggaaagca
     3001 tatttcccac aaaaagcgaa tccacttttt cgatgccagc atcttgcagg gcggcaaagg
     3061 cggcttcacc ggcaatttca cggagggatt tttcccaatg ttcgtcgatt ttggtttgac
     3121 caattccaag gacggcaatt tttctcatac tgcaaattct accgcattgc cctatcaaga
     3181 actatccaaa atggaaggaa caaatcatga tttcgggtcg tccatttttc attacaatct
     3241 caaggagaaa acatgttacg ctcttatttg attagtttgt tcgcggccgc tctatttctg
     3301 gcgggctgcg ctcaatcaac tgcgacggcc tacccaaacc ctgaaactgg tcaaaacgcc
     3361 atttccatgc ccagcctgca aggagactgg cggattaagc ttacccattc aggcggcatc
     3421 atgggtttgc tgcggacaat ggaaatttcg tcagatggaa gttacacagt gaccgacgaa
     3481 cgctcaagcc aaactgtaac aaaaactctt ggttcggaag agttgcagga acttaccaga
     3541 cttatttcat ctgcacaata catcgctccc agcggaccca atggaatggt ctgtgcagat
     3601 tgttttgtat atgacctgga aataaaagga agcgactcaa cttttaccat tcaattgaac
     3661 gatatcagcc tgccagactc agggatggct cccgtaatcg ctcacttgag agatctgatt
     3721 gacaaggccc taaaatagaa atggaccaga ctgaatggcg tgaaaagttt ctggaagccg
     3781 ttgccaattg ttgcagcgaa gggaaagaag ctgtggaatt cattcacgtt cacaaagtaa
     3841 ggattggaat taaaccagcc cgaaaaagcg tgggggcatt ttggactctt gaaaaacggg
     3901 cttacttaaa ttcggtttac ttttcacccg agtcttcact tgccaaccca aacgcctggg
     3961 ttttactcat ccatgaggtc aggcacttgc agcagggatg gagtacagcg ttatccattt
     4021 atggagaatt ggacgcatgg caatatcaat tcaacacact caaaaagatt acctccgcca
     4081 aactccctga ggcggttgat gaaattctgt ctctgccatt gagcatggat cgggaaacct
     4141 tggggcgtgc ccgcaaattg atgacccaac atgcagggaa agggtatggc gcaaatctac
     4201 ttccgcttta cccgatccac atggaaataa aatattggat ttccccggat tgatattaat
     4261 ctacgcgacc tctttcaaaa ataacaggta tttttaatgt actggctcaa cacctgttgt
     4321 atgctatgta cagataaaga aaggaggttg cgatagatac accgcctgaa ctacaacaaa
     4381 atggaaaggt cgagtcgcgt tgacctggtc caagcccaaa cttgagtacc agacgttccc
     4441 ctaacgatat aacggttgca acttcgttcc tttccaaaac atagagaacg cttgacggac
     4501 gggtaaactc ccgtccgtct ttttttgttc tgaggaaatt cagaatttca ccaactacaa
     4561 ggcagaaaaa gccttctgca tcttttccaa accctgttca agaatttttc gcgatgtacc
     4621 gaaattcaag cggacatgcc ctgccccttc atttccaaaa atcttccctt cgctgagcgc
     4681 cactttggcc ttgtctttga aaaactcgta gggcgagcca tcaattgccg tttgggtgaa
     4741 atccaaccag cccaggtatg ttgccgaagg caccgttgtg cggactccgg ggaaattacg
     4801 ggtgacatat tccactagaa aatcacggtt gccagtcagg taggtctgca attcggccag
     4861 ccatggatca cacttgccag aaaaagcagc tcgggcggcg tgcagcccca tgcttcccac
     4921 gtgcaggcgc atgtgctcca tttcggttgt gtaacgttca cgcaatgact tgttcgggat
     4981 gatcgcaaac ccacaaaaca gcccaggcac attgaaagtc ttggatgggg caaccagggt
     5041 gatggtatgc tgagcgatct ccgatgagat ctttgccatc ggggtgaatt tatttccatc
     5101 caacatcaat tcagaatgga tctcgtccga aacgatcaac accttgttgc ggatgcaaat
     5161 ttccgccatg cgggtgagtt ccttgcgcga gaagataatt cccaacgggt tatgcggatt
     5221 gcaaagcagg aagatgcctg cttttttcac ccgcttttcg aacagatccc aatcgatttc
     5281 gtaactgatg aggttccctt tcacgttttt caccagcgga atatccgtct gtggaattcc
     5341 aacattattc ttcacctgat gaaattcgtt gtacacaggc gtttgaattg caatgccttt
     5401 tttcggtgtg caaaatgcac gggcggctac tgagaatccg ctgacgatgc ctgttacagt
     5461 tacgaccgat tcgggtttaa atttccagga atacaaacga tccatacgtg cggcgacggt
     5521 ttcctgcaaa acagtgccgg ggatttcgta tcccaacacg ccatgatcca ctgctttgtg
     5581 caatgcatcc aaaatcggtt tcggtgcggg gaaatccata tcggccaccc acatgggcag
     5641 gatatctttt tcgtaccagg tccattttgt gcgattgaaa ttctttctac gatcaggtgt
     5701 gatattgaag tttgtcatag atccttgtgc tggggtatgt tgtcatcatg cccatggtat
     5761 gaaatgtgga cacaccaatg ctgtgtccta ttttttatac gcgacgatca tcccgtcacg
     5821 cattggcgtg agtgagacga tccaatcgcg gtctgacgta atctttctgg tgaattcctg
     5881 cacgcccatc gtggcggacg agacatcctt ttcatcaaaa atacgcccat gccagagcat
     5941 attatcgata ataagcaacc cgccgctgcg caatttattt ttgatctcgg gcagcgatgc
     6001 agggtaggcg tgcttgtcaa tatcgttgaa aatgatatca aacggacctt ctgtctgacg
     6061 aagggtctca accgcctctg ccatatgaaa ctggacaagg tcggcgaatc ccatcttaac
     6121 gagatgtttg cgtgccctct tcgacagttc ctcgtcccaa acagtgtgat gcacaacacc
     6181 gccgccattt tccacgagcg ccttcgcaaa ccaggccgtg gaatatccgt agcccgaacc
     6241 caactcaaaa atagactttg cgttgatcat ccgcgcgagc tgatagcaat aatatccaca
     6301 cgcgggcccg atgatgggaa aatcgttttt ctcggcgtaa gcctccatct ccagcaattc
     6361 tgcttcacgt gatggaacaa gggatgcgag ataatcctgt accgtttgat aatccaattc
     6421 actcatggct acctccaaag tatttttcgc ggaaacnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnaatt caaggcaacc cgccccaata attggtgata gaataccaaa
     6601 ggccgtttgg cctttttgaa tgtacttatc acatgcaagg atctcgccct gaatgaaata
     6661 taacttccgc ctgttttggc gcaccttcta tcgctcattc tttgactcaa aaaacactcc
     6721 agcccgcctc acccgcaaaa gaatcacctt ccttatcctt ttctatctcg tctggcccat
     6781 cggcggattg atacactggt tctgctttgc gcttgatgac atcctcttcc ccgcctacaa
     6841 aaatcacccc atcgaaaaac ctcttttcat cctcggcaat ttgcgcagcg gatcgacctt
     6901 tttacatcgc ctgctctcac gagacacaga gaccttcacc agcctgacca catgggatat
     6961 ctacctcacg ccctccgtga cccagaaaaa attcacacag cttattgcaa ggcttgataa
     7021 caaatatctt ggcggaacct tacacaagct gttgtacaaa ttcgacgccg ccaccctcgg
     7081 aaagatcaaa attcatccca tctccttctt tcaacccgaa gaggacgaga acatccatct
     7141 gcatatctgg gacggctact tcgtcacctt cctcttccct ttcatggatg aattccccaa
     7201 ttaccagcat ttcgacgaag ccctcagccc tgaacataaa aaacgcatca tgacctttta
     7261 ccgctccatg atccagcggc acatgtatgc gaacggcaaa aaatatttcg tggccaaaaa
     7321 ccccgccttc agcgccaaga tcgaaacgct cgccgaattc ttccccgaag cccgcatcat
     7381 ctatctcgcc cgcaacccgc tcgacatgct gccctccacc atctcgtgga tcaattatgc
     7441 caggcgtcaa ttcaccgacc caggcgaagg attcttttac attgaagaaa tcgttgacat
     7501 gaccaaacac tggtacagtc acccgctcca atatttagac tcgcatccct cgccccgcca
     7561 ccttattatg aaatacgatg acctgatcca gcgtccagag accgtcatcc gcgccttcta
     7621 tgaacaattc ggctatcccg ataaacctgg cctgccagtc atcattgacc aggccgtgaa
     7681 agaaactctc acctttaaca gcgaccatgc ctattcctat gaaagcatgg ggtttactcg
     7741 tgaacagatc gttgccatgt accctgaggt tttcaagcgc ttcaactttg agacccgcga
     7801 accattcatc gaatcggaat taatgaaagt agaaagaaaa aaatagcgca tctgccaaaa
     7861 cgaactcgcc cgcgaaagat caatttcggg gcgagttcgt ttttaagcgc aaattgggca
     7921 caaaatcaat tatggaaaat ataactgaag caaacgtcct gcctcctggc cgtacgggcc
     7981 agcggggtcc atatttttca cttcattcaa tttttcaaaa gccgcatccc gatcacccat
     8041 tgaaagatag gtaagggcca gatgaatatg tgcctgcaaa tgatcgggaa aacgcgttat
     8101 caaatccaca aagatgtttt cagcgttggc aaatcttcct gaagagagat aagcccaacc
     8161 caatgcatcc agagtgagcg ggtcattcgg cgcaagttcc actgcttttt ctgcggcagg
     8221 caacccaatg tcttccatgt gaatggtatt ctcagcacaa aaaacagcca gcaaacgcca
     8281 gtaggttggg tcatcgggcg caagctcagt ggcatgttga taggccgcca acgcagaaac
     8341 aaggtcaccg cgtttgatgt aggcgttccc gatctctgcc tgccaggcag gattggtcgg
     8401 gtcgtattcg gcagccagca aatactcagc cagcatttgt ggatacttct cctgacgccg
     8461 ccagtacaac ccgcgcaaag cacggaccgt tacagactca tggtcgagga ggaccgcctg
     8521 atccaactcg gccaaaccga tgttctccgc atcactctgc gctgattgct gcttcgcttc
     8581 gccaagccac gcccatgcct cggcattttc agcgttaagc gcaatggctt tttcaaatgc
     8641 agcaaccgcc aaatcccatt cctgcacaag gccgagggca cggccaatgt gaatcatttg
     8701 ttccgatgca tcgggttggg tggcagataa attcaaggcg gtccgcaatg tctgcaccgc
     8761 aggatcaaat tcagggttaa gggaggaagc gagcatcaaa tccgtgagcg cctgctcggg
     8821 ttcaaggaca ctcaacaaga ggccgagccg ataacgggcg tagacatccc cgccgtttgg
     8881 atctaaacgc aattgcgctt ccaaagcggc gcgttctgcg ggccagtctt ttttctgacg
     8941 ataggcaaag gccagcaaac catgcagcat gggtgcctcg tgggtttgca aaccgcgttg
     9001 aagtaccgcg atcgccgaat ccacctcacc cagcgacagg tacgaggaac ccagccagcc
     9061 ccagccttct tctgaaagtg tgtcaccagc ctgatctaaa tatgtgatgg cttgcgccgt
     9121 atccccgcct tttgctgaag caatgcctgc cttttcccac aaccctgttt gccagaaaag
     9181 aagccttgca gaacgctcgt aggccttggc ggcttctgca taattttggg ctgtaaagtg
     9241 ggcatcggct tcttttagcg aattctggcc tgccgcgaca aatgcaaaaa aagtggccgc
     9301 agcaacgacc acgatgacga tggggatgag aatttttttc ttaagcattg caatggtaat
     9361 tatacgtgag cgaaccatat ttttttgaac tggaagtata ataatgtcga catgagccct
     9421 ttcaccagtc aaatctttga attactcaca gttcccatca acaacctgct ttattacatc
     9481 gtgctttcct acgcggtggc aagtgcgatg ctttcggcat ttatccattg gcgggccagt
     9541 gaatttccgc aagcccgccg cgcgctgatc ggtttggggg tattgcttgc cgcacaggtg
     9601 accatgttcc tgttcagcgg gttgggctgg cagggattga tcgacccaaa gatactgccg
     9661 ccgatggacc gtgctttcat tatctttgga attatttgga tcacctggct ctatgccttt
     9721 cctgagccca gccgtccagc cgatgctgct gcggctttgt tgagcgtttt ggtgctgacc
     9781 gcgcttgggc tgagtttgct tgcctggcaa ccccagatca caaccatcac ctacaaccaa
     9841 acctctgatg actggttctg gcaaattggg tcgttagcgg ttgggttgat cggcatcgcc
     9901 atcatgttcg tccgcaagcc agatggcatg tggaacggca ttgttctgct tggcttgggg
     9961 atattggggc atatcggtca tctggctttt cccattgacg gaaattactc gggcgttgtc
    10021 cgcattgctt atatggcggc ctacccgatt ctgctgacat taccgttgcg tttttccaca
    10081 cccagttctg caaacgaatc accagcacga cagattaccc aaaaacagga cgaaaacctt
    10141 ggggaacgca gacggtacag cacagacccg aagaccttcc atgccatgct ggcacttgcc
    10201 gcagaaacca accccacgaa ggtcagtcag gctatcaccc gtgccatcgc acagaccatg
    10261 ctctcagatc tgtgctttat gatttacatc aacgataaca gcaaccaaat gtccattgca
    10321 ggcggttacg atttgattcg tgaagacgct ttggaaggcg gcacgctgaa caaaggctcc
    10381 attcccatgc tggcgaattc cctccagcgc ggacgaccgt tgcgaatgcc atccagcagc
    10441 acctctgcgg acattaaggg gttgagcgat attcttggat taaccaaccc cggtcatctt
    10501 ttgagcgtcc cgattctcac cgctgaaaag gaatctctgg gcggcatcct tttgctttcg
    10561 ccctactcag accgcacatg gacagctgaa gatcaggcct acctttcaaa catcgccacc
    10621 tcactggtgc cgatcatcca acgcggacaa aaggtcaaca gaatggaagc gcagagcagc
    10681 gaagcaattc gaagatctgc cgaccttgag caccgcgtac aggaattgac cgaagaatta
    10741 cacaacgcgc agacggagat ccaaaaagca ggctctgtgg aaatggcatc cctgcttcag
    10801 gcacaggaag aatcgcagcg gattattgaa cagttgcagc ttgaaaacgc ggaattgcgc
    10861 acgggaaaga gtttgcaaac aggcgcagca cccgcttcat ttgcctccgg tcagatcgag
    10921 caggaattga aaaccaccct gcaagaggtg gcccgcctgc aaaaccaact catggaagct
    10981 aatatgaaaa cccgcgagtt ggaaaagggg catgtgacgg tcaagagcac agaacaggcc
    11041 gaagtggttg cctcgatcgc acaggaattg cgtcagccac tatcatccat tgtcgggtac
    11101 accgacctgc tgttgggtga atcggttgga attttaggtg cgttgcagcg caagtttgtg
    11161 gaacgtgtca aatcttccac ggaacgcatc cgcagtttga ccgatgacat gattcagatc
    11221 accaccctgg aagcagaatt gaacgacctc aatcctgaag ccgtggatct gaacgcgatc
    11281 attgacaacg ccatgtctta caccagtaca caggtgcgtg agaagaatat ttccatgcat
    11341 ctcgaattgc caaaacaact tgcccccatc cacgcagatc gtgaagcctt acagcagatc
    11401 ctcatccact tgctgcaaaa cgcaggggcg gcttcgccct tcgaaggcac ggtcaggctc
    11461 aaggtccaga cccgaaacga ggagaacaca gagttcatcc ttattcaagt aaccgacagc
    11521 ggcggcggca tcccagccaa agaattgccg cgtgtgttca cccgtctcta ccgtgcggac
    11581 aatgtcctca ttcaaggtgt gggagatacg ggggttgggc tttccatcgc aaagactctc
    11641 accgaagcac aacatggccg catttgggtt gaaagtgaac aggacgtggg ctcgaccttt
    11701 agtgtgttgc tacccatcgc gggcagcgca gagaccaacc cgaaaaccaa gcctaaagga
    11761 aagaaataat gcaaagcatg cgcgacctca gaaacgcgct tgtggtagcc atgatctcga
    11821 tcggtttgac ggtcgggtca ttatccattt ccctggtgga atttgcccct caggcggttc
    11881 ccagtgccac aaatcctttg caagttgtcc cgccactgct gaccgccacg cccaccgtcg
    11941 tcccctcaat gactgcgacc cttgggcttg aatcgcctac gcccagcccc acatccacct
    12001 tcacagccac tccaccgccc cccaccacct gccctccgcc cacaggctgg acccagcttg
    12061 tggttcaagc ggggcaaacg cttgatacca ttgcccaaca ataccgcacc agcaaggaaa
    12121 acctgcgtgc tggaaactgc ctcatcagtg acaaccttgt ggcaggctcg atcatgtacg
    12181 cccctgccgt aatgaccagc accgtccccg cttgtgtgcc aggtgctgct ggttggaaaa
    12241 agtcatacac tgttgtgtca ggtgatacgc tttacgccat tgccaccaat cattccacaa
    12301 cagcaggctt gctaaagagt gtaaactgcc tgagcctcga cgatatctac ccgggcaatg
    12361 ttttatgggt gccagatgtg cccacgcgca cacccaaccc cactccatta cctggcagca
    12421 cagtgacccc gatcccgacc cagttgttga cggaaaccgc cctgccttac actgctacga
    12481 cactccccag cgtaacgccg acggaagtcc cagcgactcc caccccgacc acgcctgcca
    12541 cagacacacc gacaactgcc cccaccctga cggcaagccc aaccccattt cccccgtaaa
    12601 aatcacccct acggatgatc ttttcgcgct gattcaaaca acgcctacct agatgaaatc
    12661 caattccgcc ttttcgacca tcgcgcttct ggcgtttttg ctctcagcat gtggcacacc
    12721 cgccgaagcg gttgtgcaaa cacccctgcc gttcgtctta atcacatcgg cgccaaaccc
    12781 atctcccaca ccaacacctt tccaaccatc gctgaatacc cctacacagt ctgcaattct
    12841 gtctgcagca gtggatacat ccacacctgt accgacggtc atcctgccca cagaaacgcc
    12901 ttcgcctact ttaattccac ccaccgaatc aacggccaca gtggatttca accagctttt
    12961 ccccacagtt gctgccccgc ccgtggttga agtagtggcc gtcacaccca ccgccctgcc
    13021 tcccctcacc gataacgaaa cggtcaactt cctgctcatc ggctccgaca aacgcccagg
    13081 ctcctcctac cgcactgaca cattggtcat tgccatcgtc tggccaaagg aaggtcaggt
    13141 ttcgctcatc tccatccccc gcgatttgtg gatctacatc ccgacagtgg gaatgcaacg
    13201 tatcaacacc gcctaccaaa gcggagagat cggcggctat gccggcggcg gcgctggctt
    13261 gctaaaagac accatcacct acaacctcgg catccgcatt gaccataccg ccatggtcga
    13321 gttcgacgga ttccgccgaa tcgtggatac ccttggcgga attgaagtcc cagttgcctg
    13381 cgcctacacc gattggagac tgatcgatcc ctcgctcgac atgtacgatg aaaacaactg
    13441 gtggctctac acggttggcc ccgggcaggt tcacatggat ggcgatcttg cactctggta
    13501 tgcacgttca cgctccaagt cttccgattt tgaccggggc cgacgtcagc aggaagtact
    13561 gcgaaccatt ttccaaaaag ccctgcaaac tgacaccttc agcaaaatcc cccagcttta
    13621 cgaagacttc tcaagcacca tcctcaccga tatgggactg gcagacatgc tgctcatgtc
    13681 accatacgcc gtcaacttca ccaacgccaa catccgcggc tatttcatcc gccctcccta
    13741 cgtcacttcg tggacaaccc ccgcaggcgc ggcggttttg ctccccaatg aaaccgagct
    13801 caaccaaatg ctcctcgaag ccaccacact ttccactttt gccgaacagc gcgaaaccat
    13861 caccgtcgaa gtgcaaaatg gctccccgtt cgacaccatg gaaagcctcg ctgcctcacg
    13921 cctcaattac gcaggctatc aaaccatcct ctcgaatgcc gaccgccgcg actatgccaa
    13981 ctccgtcctg gtggatttca cccccgggca ggatgccacc caacgagaag cgatcatcgc
    14041 caccctcaac ctgtataccg caaatgtaat ttccctcccc gatgcaaaca gcacggtgca
    14101 atatcgagtc atccttggca gtgactatca ggcctgtttc cagccacaag acctctcaca
    14161 ttgaaaaaaa acggcatccc cgccgccaag aacggattaa atcaacatta cagtttcaat
    14221 ctcggcagtt gacaacctct cctatattcg ctaaactata cgaaaagact atgcgctagt
    14281 acgtaaggaa gaccttatgg caaaaacacc tgattccgct catatttcag ccaagaccct
    14341 gctccctgcc gccatccaag gctgggaaat gttcatgctc gatcagggac gttccacaaa
    14401 cacggtcaag gcttttcttt cggatgtccg cctgctgact caattcctcc ccccagacca
    14461 atccatcggg tccatcacga ccgaggatct caatcgcttc ttcacatgga tggaaaaaga
    14521 ccgcggcatt ccatgcagtc ccaagaccct ttcacggcgt atcacctccg tcaagtcatt
    14581 tttccgttgg ctccaccagc acggctctct ggttcttgac cccgctgaaa aagtgttaca
    14641 acgctctgcc atcagcccgc ttccgcaagt actcaccgac gacgaatacg aggcagtttt
    14701 gctggcagca gaccgtctcc gcagggcttc caaaccagat gcccgctcct acactttggt
    14761 atacctgctg ctcacgacag gcatcaaaaa aagcgaatgt cttggcatcc acatcaacca
    14821 cattgacctt gaagccaaga atggacccca tgtattcatt cgttacgcca gccctgccaa
    14881 ccgctacaag gaacgcaaga tccaactggc agaagactgg ctcgaagcct ataaagaata
    14941 tctcgcccaa taccaacccg ttgaccaggt ttttgcatgg tcgccgcgca ggttggaata
    15001 cctcctcgaa gatatcggaa atgaagctgg cctcaccaag cacctctcct tcgatatgtg
    15061 ccgctggacc tgtgccctgc gtgattatca atcaggcatg gaagcagaca acatccgcca
    15121 aaagcttggt gtttccaaaa ttcaatggcg cgagttgttc atcaagatca ggcaattgaa
    15181 cggagagata aagtaatcgt ccaccgctaa aaatacctca agaataaaaa attcccgctg
    15241 actagagcgg gaatttttta ttcggttatt ttctcaagtg ggcccgaaac agaaaaatat
    15301 atggcggtct tggtggattg tgtcagacga taggaatgtg cagggcgata ccccggcacc
    15361 caaatgatct ggtcgccagc gcacaacaag ggccaatgat ctctggcacg ctggggcatt
    15421 ttctcgttga caaagaaatc ggaaagcttt tgcgaatgac cttccaaacc aagcggttcg
    15481 aaggtatctc cctgatggcg gactcgcaat tcaagcatct cgggaacccg ttcggcatcc
    15541 aaccagatct ggaacggatc ttcattcctc tcagcctgct cacgtaccaa tgtgggaata
    15601 tgccaacgct cgcagctaaa ccgccaccca cctgcaagtt ccacctgccc aggaattgcc
    15661 acctgaacgg gggttgtgct gggcatttgc ggccagaggt tgaaggggag tttcgaattt
    15721 tgggtgcaaa cgtacaaaac acccgcttcg cggaacatgc gcaaaccgcc tttcaaatcc
    15781 acgctggagg agtgggaacg gtctttgatc aggtcggttg cccgttccag ggcggaaaag
    15841 ttcacatcca gctttgggcg cagaatctgc atggcatgtt tgaccagatt ccgctggagt
    15901 ccgatcgagc ggttggcaag caggtcggcg tcaaaggcaa tggtatcctc ttccacactg
    15961 gtaacaactt cctgccatgc attttcgagc gtttccatca ggagagagta atcactcttc
    16021 agcgattggg acatgcgcag cacgacatcg cggaattttg ggttgtagct ttcgaggctt
    16081 ggaatcaaaa ggtggcggat acggttacgc tgaaaattga tcgaatcgtt gcttgagtcg
    16141 taatgtgggc gcaaaccgtt ggccgcacaa aagatggtgg tctcttcacg ccaggtgttc
    16201 agcaacgggc gcacaatggg aatcttgggg tcaaatgttt tgatgatgga gcgatgcgac
    16261 attcccttta aacccgagat gccgcttcca cgcaggaaat gcatgagaat ggtttccacc
    16321 tggtcatctg ctgtgtgacc taccgcgacc gcctgtgcgt tcctttcacg tgccaattta
    16381 aacatgaagt ggtatcgcaa attacgggcc gcttcttcca gtgacagttt ttgctgatct
    16441 gcataagcgc gcacatctgc gccatcaatg atgcaaccca gcatcaggcg ggcggcgatc
    16501 ttttccacca tatgggcatc ctgcccggct tcggggcgga gttggtgatt gaaatgcgca
    16561 ataatgatgg aatagcccgc ctgcctcaag gtttccatca agcacaaact atccggcccg
    16621 ccagagaccc cgacaatgat cgggcggtct ttaacgaggc cgcattcgtc ctgcagggtg
    16681 gcgtccatgt tttccagcat catatcttta cttcgcttta attgtgtgct ttacaaaaac
    16741 cacagagggg tttctgcatc aaagccacgg ggctaaattt gatataactc caccaacacg
    16801 ccgcccgtgg attcagggtg gatgaaggcg tatttttttc cgtcaaccgc ggtgcgcggc
    16861 tcttcgttga tcaagcggat gttcttcgcc ttcaactgtg acatcattcc ttcgatgtca
    16921 tccacttcaa ggcagaggtg atgcatgccg ggtccgcgct tggccagata cttggcaatt
    16981 cccgaatcgt cagaagtggg catcaccaat tccacttcag cgcctgcaac gggcaaaaaa
    17041 gccacctgcg atttttcagc agggacatct cgtagttcat gcaattgaat tccaagcgca
    17101 tcgcgccaga acaaaaggga tttttccatg tcgtccacga cgaccgcaac atgattgaca
    17161 gctttgattt ttggcatagt gttcctcaac ggttggattt tacttccgat taacgatttc
    17221 aagcgccacg gaaatgacct tttccaccca catggagtac atcttccctg atggatgcaa
    17281 acggtctggg gcgagtaatt caaaatcatc cattgccatg cgggagatct cagtgacatc
    17341 cacataatgt gccccggcgg atttggtttc ttcaaggtta actgcattaa atccgtcgat
    17401 ttgcaggctg atcggttcgg tttcgcgggt ggcggcaaag ggggtaaacc cccaatcggg
    17461 aatggataac acaatcacat gctgtggatc gcccccggcg tattcaatgg ccttgccaag
    17521 cataaagcgg aaatcctccc gataaccatc cagcggataa ccgcgatact ggtcattcac
    17581 cccgatcaag agtgtgacga gatcatacct tccttcgggc ggggtgactt gaatgccctc
    17641 ccaaagttca tccacggtcc agcctgtacg ggcaatgatg gtggtctcgg catgaattcc
    17701 ctgttcttcc aatttggcag caagttgatt cggccaacgc tcgctttccc tcacactttc
    17761 accgatggta taagagtcac cgagagcaag atagcgaatt tccatttttg ttgcattctc
    17821 cacggttggt tctaaaggtt gggaacaggc aaaaagaaaa acagacagta caaatgcaat
    17881 ttttctcaca aggcaagtat atcaaaaatc gtcccagcca gtttggcagg gacgattttt
    17941 ttcgaatggg tgagagaaga actaaagtga gcggaccttc agccacaagc tcggaattcg
    18001 gctgatctta cccatggcag aaaggctggc gcggcgattg aatacgttgt aatcgtgttt
    18061 ttcgatatct tccaaaatac cgcaataaaa agtggatgct gctccgatgg ctagctggcc
    18121 ttcacgctcg agcattttca cgccttccca ggattcttca tatagctggc gggtacggtc
    18181 gatctggaat ttcatgaact ggcgccaacg atctgtcact tttccttcgg caatatctgc
    18241 ttcctgaata ccgtagaaga caagttcttc gcgtggcagg tagatgcgcc cgttacggta
    18301 atcctccccc acatcgcgca ggatgttggt catttgcagg gcaacgccaa gtttaatggc
    18361 gtagggcaca gcatcatggc ccttgaaacc gacaatgtac atgctcatca accccacagt
    18421 ggaggcgaca ccgtagcaat aagtggcgag gtcttcaaag gtttggtagc gggtttggga
    18481 aagatcacgc gccacgccgt cgattaattg cagggcataa tgatgcggaa tatggtatcg
    18541 ggtaagggta tctgcccagg cggaggcaac cgggtcatcg gtcggggaag aagcggtctt
    18601 tacaatgttg cgccaataat cgagttcaaa gtcgcggtct tttttcaact cgacttcatc
    18661 cacaatatca tccacggttc ggcaaaaagc atacaaggca cggacggcgg aacgtttttc
    18721 ttcaggaagc agcccggaag ccaggtagaa agatttggaa tgttcactgg tgatctttgc
    18781 ggccttttta taggcggcgt gcagttgggc atctccagcc cagtacgaaa agaagggacg
    18841 ggcagaagtg tgggggaagt gccccgcgag ggcgatcagt tggctttccc aattcgcttg
    18901 tgcttgcatg ttctctcctg tttttagttt ctctgagtag ggctaaaaca acgtgctgat
    18961 gaaaatattt tacaggatat gtctattttg tcaaggtttt gcaaagtatt tttcaagcaa
    19021 accttgacaa atactagaca aagcatataa taacctaaga cgttttttga acctcaagga
    19081 gaaaattatg cgaaaacaga ccgcagtagt gattggtgca ggcatcggtg gaatcgccac
    19141 tgccgcccgc ctcgccaaaa atggatacga cgtgaccgtt gtggagaaaa atgacacccc
    19201 cggcggacgt tgcaaccaga ttattcaaga cggccaccgc ttcgatattg gccccaccct
    19261 gttcctcatg cctgaggtat gggaggagac ctttgccgcg ctcggcgaaa aactgagcga
    19321 ccaccttgac ctgcgccgta ttgatccaac ttacaaggtc cgctttgatg atgggctgca
    19381 gcttgaattg acttcgaaca tcggtgatat gcaaacccag ttggaaaaag tggaaaagac
    19441 cgcctttact ggttttttga attacatcgc tgaaggcagc aaacattaca aaatatccct
    19501 cgaaaagttc gtgggacgca atttctacaa tatttttgag tacttcagcc tttccaattt
    19561 gccgctgatc tttcaactca aggcgttggg caagcattac accaacacag gtcgtttctt
    19621 caaagacgaa aggctcaaag ccgccttcac tttccaaaac atgtacctgg gcctcagccc
    19681 atatgatgcc cctgccacct actccctgct gcagtacaca gaactggcag aaggtgtttg
    19741 gtacccgatg ggcggcatgt atgccggcat tcaggctctg acccgcatcg ctgaaaagtt
    19801 gggcgtaaag ttcatctacg atgcgcctgt gaagaagatc aaaactggaa aatcaaaagt
    19861 ggatggcgtg attctcgaag atggccgtga attgaccgcc gatattttcg ttggcaatgc
    19921 agacctgccc tacatttacg accagcttct gcctgaccgc gccgaagcga aaaaactcat
    19981 cgacgaaaag ctctacacct gctccaccat catgttctat tggggcgtgg ataaggaata
    20041 ctcccagatc gcgcatcaca acgtcttcct tggcggcgaa tacaaagcct ctttcgagaa
    20101 gatcttcaac gaacacaccc tgcccgaagt gccatcgttc tacgtccatg cacccaaacg
    20161 caccgaccca gccgccgcac cggaaggaca ggacaccctg tatgtactcg tccccgtcgg
    20221 tcaccttgac gcccgttctg cacaggattg ggatgccctg gttgcccgcg cccgccgcta
    20281 cgtgcttgac cgcctcgcga aagaaatgga cgcggccgat cttgaagcgc atatcaagtt
    20341 tgagatcgtg taccagccca aaacatggaa ggagcgtttc aacctcgtca acggctcagc
    20401 ttttggccta agtcacaatt tctggcaggt tggttacctg cgcccgcaca accgtcacaa
    20461 aacctacaaa aacatgtact ttgcaggcgc atccacacac ccgggcacag gtctgcccat
    20521 tgtcttgctc tcagcccgcc tgaccaccga acgcattctc aaagaattta cgggccaaca
    20581 agtagaaggc tacaaactgg aaaaagcagt tgcacaaggg taattcggca caaagaaggt
    20641 acaatagccg tgcaaaacct ttgcagctca cccaatttca gcttgtaacc ggagttcatc
    20701 atgtccatca gcaaaaaccc cgcctttaac ttaaaagtcg tcctgcaaga aacaggcatc
    20761 gccgccgaca cgctgcgagc ctgggaacgc aggtatggca tccccatgcc acaacgcacc
    20821 gcaggcgggc atcgtttgta ttcgcaatat gatatcgaga cgatcaaatg gctgatggcc
    20881 cgacaagccg aaggactctc catctctcgt gcagtggatc tgtggaatga acacaacgct
    20941 tccggcgccg acccgttggc tggttttata ccaacaacaa acatcgcccc gttggcgact
    21001 cctgccatta ccctttcagc cgatacaagc cttgactcca tgcgggcgca atggatcgac
    21061 gcctgtctca atttcagtga agccactgcc gagcaggttt tgaatcaagg cttttccatg
    21121 tttccagtgg aagtcatctg caccgaagtg ctgcaaaggg gaatgtctga gatcggcggt
    21181 ctttggtacg aaaaccgcgc atcggttcaa caggagcatt ttgcgtcagg cctggccatg
    21241 cgtcgactcg acgccctgat cagcgcttca cctgcccctt ctctcaaaca gaccgtgttg
    21301 gtgggatgcc cccccaatga atggcacacc ttcaccccgc ttttgctctg cctgttactt
    21361 cgccgccgcg gctttggcgt catttacctc ggagccaatg tcccagcgga gcaatttgaa
    21421 gaaaccgttt ccagtgtgca tgccaatatg gtcgtccttg tggctcaaac gctcattacc
    21481 gcggccgcct tacattccac tgcactttcc cttgtcaaca tgaaaatacc agtcggcttc
    21541 ggcggccgta tcttcaatat tcatccagcc ctcaaagaat acatccccgg tcattacctc
    21601 ggcaacacag tgaccgcctc cgttgagcgg atcgaacaac ttttgaaggg caaccagtcc
    21661 aaggttcagg caaaaagcgc caccgaagct catcacgttg cgttggagca tttcaattcc
    21721 cagcgtttac acattgaaag cagcatcaag gaaatgcttc cgctgttcgt cgccaaagac
    21781 attgacaccg gcattcactt ccttggcgat aacatcgccg ccgccctcca acttggtgat
    21841 atggagcatg tcaccgccga attggaatgg ctgaaagtgc tcatgcaatc gcaccaacgt
    21901 cctgccagcg aactggcgga tttcatgcac aattacgcca cggctgtgga taagcacatc
    21961 aatggccgcg gtgaaccgat caaagtctgg ctgaacgaac aagccagact cacccagtta
    22021 tgacaaaaca agacatcctt tcaacctttt accataaact tttacctcta ttccccccgc
    22081 ggaccgaagg ctacctcaac cgcctcaccg cgcaggattt acagcactcc aattttcgtt
    22141 acatcttttc cgtgcaaagc atgatcgaga tcagtcacat gatcgaagac tcggtcatta
    22201 acacgcccgg cgtggcggag acgcacgtct ctttccaata tttttcacgc attcctgctc
    22261 aattggagca atacaggaaa gtgtcagaag catccactgg tttgtggttg tatggtgtgc
    22321 cagatgcacc cctgccccag ctttcccgca ccatcggcgt cagcacagat gggactccac
    22381 tggaaaccta ttggtttgtg gttgcctatg gcccaggtct ttacgccacc ctgctcgcag
    22441 aagagatcac cccggaggaa ggccagcgca tgtacgaagg tttttatacc tttgaaccag
    22501 agacagcctt tcaagtagtt tcactgctgc accaaatgtt ccccatgcag gtaccggcgc
    22561 cgattgcgcc tcaagaccag gagcacgcct gacatgaaca tacgcatcgt cacagacagc
    22621 acttgcgatc tgccagatga agtcacaaag cagcaggcca ttacggtgat tccactgaac
    22681 atcaatattg gcaatgacac gttccaagat ggcattgacc tcacccgtac tgaattctat
    22741 gcccgcttgc cagaatattc cccctcacct aaaaccgcag cgcccgggcc tgatgttttc
    22801 aaaaaggaat acgaacgcct tgctgatgaa ggagcgcgat cgattctctc gatacacatc
    22861 tcagagacat taagcgccac gatcaactca gcgcgcaccg ccgcggaaca attcacccgc
    22921 atcccggtca tcgtgctcga ttccagccag cttagccttg gcacaggctt tctggttgaa
    22981 aaagctgcga tcatgggcgc agccggggaa acgatggaga tgatcgtctc aaccctgcaa
    23041 aaaatgatga agaggactta cgtctttgcc tcgctcaaga cacttgaata cctccgccgc
    23101 agcggacgaa tgaatttcgc gcttgccagt ttcggcgaac ttttacagat caaaccgctg
    23161 ctgcacatgc atcttggaaa agccaccgcc caccgcacgc gcacccaaaa acgggcaacc
    23221 gaacgtctgc tttcctggct gaatgaatat gctccctatg aaaaactggc cgtcttgcat
    23281 gcaggggcac aggatgaagc cgaggcactg cgagaaaaag tacgctcgtt cctgcccaat
    23341 gacgatgttt ccatcatcca gatcaccccg gtgcttggtg cgcacctcgg catcggcgca
    23401 ctggggtttg cctgcatttc aaaggaataa gctcatgaaa attgcaaaac tcatttccat
    23461 tctcggcatt cttgccatga ccgccgctct cgtcaacggg tttacccgcg gagacttctt
    23521 cggcgagggc ggtcagctgg ccgccatgcc gtggggcatt gtttcacttg tggacctgta
    23581 caccggcttc accctctttt caggctggat cattttccgt gaaaagtcac tgcccgtcgc
    23641 cattgcatgg acagtagcca tgatggtcct cggcttcttc gctggcagtc tctacgcttt
    23701 cattgccctg caaaccagcg gcaatgactg gcgcaaattc tggctcggaa aacatgcctg
    23761 aaagaacact cgcgccacag ggcaaggggc gtgaacttct tgaagaattg caaacggtct
    23821 ttgcaggccg caattccttc atggacgcga tccttccgcc gattctcttt ctggcggtca
    23881 atggcttggt gaattttcaa gccgccatgt ggagcgcact ggcccttgcc atcttctttg
    23941 ccgcgtttcg catcgttcga aaacagtcgc tgatgtacgc gctggcagga gtgggcagtg
    24001 tggcggccgc cattggaatt gcctggttcc ttggcaagtc tgagggattt tttctacccg
    24061 ggctcattag cggaagtgtg accctgttcc tgaccatttt gagtctggtc atccgccgcc
    24121 ccatggtggc atggaccagc tacctggccc gccgctggcc cctggaatgg tattggcatc
    24181 cgcttgtgat gcccgcttac agtgaagtga cttttgcatg ggctgtcttt tttgccatcc
    24241 gtctgcttta ccagttcgta ttttttcaag atgctgatgt caattcactg gcggctctca
    24301 atttcatcac aggctggcct gccacagttc tgttattggt ggtgagttat ctctacggca
    24361 catggcgact cgcccaacta cgcggcccca gcgtacagga atttctcgac gactcccccg
    24421 ccccgtggca gggtcagcga cgcggcttct aatcaacttt ataaaaggaa aatcctcaat
    24481 gaaaacaaca gatcgcaacg cactcatcgc atttcccatt ttgatattga tcggtgctct
    24541 ggtggctctg gctggcagtc agggcggcgc ttcgatcgcc ggagttccgt tatttgccgc
    24601 atcggttggg ctggcattct taattcaatg gctggttttc attcccgcct acctgatgca
    24661 gactgagaaa ttcttcgatc tgacaggcag ccttacctat atttcggtca ccatcattgc
    24721 agtgattttt agtggaacca tggatggaag gtccgttctt ctggcggcgc ttgtggtcat
    24781 ttgggcggtt cgtctgggaa gtttcctgtt caaccgaatt agaaaagcag gcaaggatga
    24841 ccgtttcgat gaaatcaaac cctccttcat tcgttttctg aatgtttgga ccattcaggg
    24901 tttgtgggtg acttttacga tggcagcggc tctggtcgcc attaccacaa gcaccaaaaa
    24961 gggtctggat gtatttgcca tcatcggcct tgtgatctgg gttttcggat ttgtgttcga
    25021 agttgtcgcc gattcgcaaa aaagccgttt caatgcaaac cctgccaata aaggcaggtt
    25081 cattcagaca ggcctctggg cacgttcacg tcatcccaat tattttggcg agatcgttct
    25141 ctggctgggc attgctgtga tcgccattcc cgttttgcag ggctggcaat gggttgcgtt
    25201 gatctccccc ttgttcgtca ccctgctgtt gacccgcgtc agtggtgtgc cactgctcga
    25261 gaaaaaagcc gatcaaaaat ggggcgggca ggaagattac gaagcctaca aaaagaaaac
    25321 ccccgtattg atcccgcgtt tgtaattcaa acagaaaaga aacatgatca ttcaaatctt
    25381 gcaaatcatc gcagccattg ccaccatcct cacagggttg gtctcgctct tcaagccaga
    25441 gtcagtgacg gggtttacgg gcctccgtcc cgaaggcgga cgcggcatta ccgagatccg
    25501 cgccgtactg ggtggattct tcgtcgcact tggcgctgca ccattgatgc ttaattcgcc
    25561 tgtcgcctac accatgctcg gcatggccta cctggttgta ggcgtggtgc gcggcgtttc
    25621 catgtgggtg gataaatctg tcacccaatc caacatcatc agccttgtgg ttgaaatcat
    25681 tttcggtgtg atcctggtgg ttcccgtatg agtcgtgctg aactcaacaa acccgccccc
    25741 aatttcacat tgaccgattt caacggcaat gagatcagcc tcacagattt tcacttgaag
    25801 aagaacgtcc tgctcgtttt caatcgcggc tttttctgac ccttctgccg acggcacatg
    25861 gcgcagttgc gccaggatta caacaaattc acagaacaag acaccgtgat cttggtggtt
    25921 ggacctgaaa acagtctggc attttcgttg tactggaaaa ccaattccct gccattcatt
    25981 ggcctgcccg accccagcca cacggtcttg aagcgctacg ggcaggaagt aaacctcttc
    26041 aagtttgggc gcatgcccgc ccaggtcgcc atcgataaaa atgggatcgc ccgtttcgtc
    26101 cactatgggc atgacgcacg cgacatcccc accaacgagg aaatcctcga attattggaa
    26161 tccatgaaca aagaatcgat tacatcgtaa aaggaattta tgaacaacaa acgcctcttt
    26221 tcaatcatcc tggtcgtatt tattgacttg ctcgggttca gcctgatcct gccgctcctg
    26281 ccatattacg cagagacttt caatgccaac gaaaccatca cagggatcct gatcgcctcc
    26341 tacgcagtga tgcagttgat cggcgcgcct ttgcttggcc gcctctcaga ccgcttcggt
    26401 cgtcgccccg ttttgctgat cagcgtcttc ggcaccttca tcggcttttt gctgctcggc
    26461 ttcgccaacg ccctgtggat gctcttcgcc agccgcattt tggatggctt aacaggcggc
    26521 aatctaagcg tggctcaggc gtacatctca gacgtgaccg acgaaaaaag ccgctcaaaa
    26581 ggactcggca tggtcggtgc tgccttcggg cttgggttca ttatcggccc ggtcactggc
    26641 gggttgctca gccaatgggg atatgccgtg ccagcgtttg tcgcagcagg catctcgttc
    26701 ctcaacctta ttctgatcta cgcctggctg cctgaatcgc ttaccgaaga aaaacgcaac
    26761 cagatgaccg aaaaacgccc agccgtcacc ctgcaggcat tgatcgttgc attcaaacgc
    26821 ccgtttactg gctccctgct catcacccgc ttcttttacg ggctggcctt ttccatcttc
    26881 caaaccatct tctcgctgta tgcgctggca aaatttgatc tcaccgcccg cgatacaggc
    26941 ttcgtgttga cctacgtcgg tgtcctggcg gtcatcgtac agggctttct cgttgggcgt
    27001 ctgaccaccc agttccgtga agacctgctc attaccgttt ctgtggtgtt gatgggcatt
    27061 tctctgctgg gttgggcatt ggctccttct gtgttgtggc tgtacatcat catgaccccc
    27121 accgccattt ctggcggcct gctcaacacc ctgctctcca gcacactgac caaagccgtg
    27181 gccccacagg agatcggcgg catccttggc ctttccgccg cagtggaaag cgccacccgt
    27241 attatcgccc ccctgctggg cggcgtgctc ctgcaacagg tcggcacctg ggcaccgggc
    27301 gcattcggcg ccgttgtgat gacgggcgtc agcctgtatg tattcaccac catttacaac
    27361 caccccattg ttgccacact gaaacaagga aagaccgccc ccgtccccgc atcggattaa
    27421 ccaatccaca ggcaatggca tgtcaggagt tgaacatgac cgataaaacc acccgttacg
    27481 aactgccacc ctcaggcatc accctgcttg gatatgtggt ccgtcgtatg cacaaaaccc
    27541 gctggctgct gccagtgccc gatttgcttg tgcagggaaa aaccgacatg gcgttattca
    27601 acgttgccat gtatgccgtt gcctcttcca catctttcgg acgggcatat tatcagccgc
    27661 aattcaactc cgcaggcgca gagatcaccg aaacagaccc gctcacaggc gcaaacgtca
    27721 ccgcaaaatt ggttgccacc tcgccgcatt atttcatcac ctacaccgcc gccatgccaa
    27781 acggcgatct cttcactggc agcgaagaga tcacaggcac caccgtcggc ctgcgcggat
    27841 tgggcatgcc tgccccatcc aaattcacct tcacatcggg cgggtacacc gccgaactct
    27901 caggcgttct caccagcgag cttgcgcttt cactcattgg ccgtacccgc atccgcgcat
    27961 acggctttct caacttcaaa gacaacgcag gcaacacagg caaactcaac ctggatcgca
    28021 gcagaaagat caaaattgat atcaacgaca aagccatgga gcccaaattc atatgacttc
    28081 gatctggtgg attcgccgcg atttgcgtct gactgacaac cttgcgcttc attctgcgct
    28141 gaaaacgggt gacgtcatcc cggtcttcat catcgacccg gcattttcct ctcaatcgcc
    28201 gcgccgacga agtttcctct acgaagggct gcacaccctg gatcaggatc taaaaaaacg
    28261 cggctcgtac cttgtcctcc gcacaggcag gccagtggac gtgctgcatc aactcatgca
    28321 ggaaacaaaa gcgcaggctg ttttcgccga ggaagatttc acaccctatg cccataaacg
    28381 tgacacccat gtggctcaat ctgtgccgct gcacctgctt cccgggcaaa ccgctcaaca
    28441 ccccagccgt gtgcataaac aggatggcaa accatacacg gtgtacacac catattccaa
    28501 aacatggaag gccatgttgg aggaaatcga actcgttccc gcgccagatg tcatctccac
    28561 accggcaggg atctccacag agccgatccc tgcttttggc gtgaatccgc tttttccagc
    28621 aggtgagcag gaagcactga cccgcctcga tgaatttgct ttcaaacgca tccacgccta
    28681 cgatgaagcc cgcaaccgca tggacctgga cggaacatca tcgctttcac cgtatttgcg
    28741 gtttggaatg cttgggctaa gacaagccgt caggttggcg aagcaggctt cggcggagtc
    28801 acgaggcgct gaaatctggt tgaacgaatt gatctggcgc gagttctaca tccagattct
    28861 gtatcatttt ccatacgtca gtcatcgtga attcaacccc gcgttgatgg ggatcaggtg
    28921 gcgaaacaac atcgaagaat ttgaatgttg gaaggaaggc aggacaggcg tacccattgt
    28981 ggatgcggcc atgcgccaac tgaaagaaac aggctggatg cacaaccgcg cgcgcatgat
    29041 cgtggcttcg tttttggtga aagacctgct gatcgactgg cgctggggcg aggcatggtt
    29101 catggaatgt ctgctcgacg gcgaccccgc tgcaaacaat ggcggctggc aatggacggc
    29161 tggcacaggc accgatgccg ctccatattt cagaatcttc aaccctgttc tgcaaagtgc
    29221 aaaattcgac ccgctgggcg aatatattcg caaatgggtg catgagctgc gaggattgaa
    29281 cgaaaaggaa atccatgcgc catgggaaca cggtgtcaaa atcagcggct accctgaaaa
    29341 accgatcgtg gaccgtgccc tcgtcaaaga aagaaccctg caagcgtaca aacaggtaaa
    29401 aagcgaaaga tgaagcctcc ccttcatgca tccgcacctt caacttgcaa cctgaaacct
    29461 tcaactacat gaattcaaaa atcacccccg aactcggacg cgcagccatg cgcgcacttt
    29521 tcgaaacaag atcgccgctc ggcccgctga aagtgatggc acagcatgtc ggccgcttct
    29581 ttcaaattcc actgcctggt tttcgaccct atgtggtctt tggcccggag gcggcccgta
    29641 aagtgctgat caccgaacgc gacaaggtgc tctggcgcaa caccgacccc gtaaccgacc
    29701 tgcttgggcg tggggttctt atcaccgacg gtaaagaaca tgaccactac cgcgagctga
    29761 tggagccgcc actacacccg tcgaatcttt ctggctatac gcaaatgatg ctcgaaaaaa
    29821 ctgaccaagt cacctcacac tggaaaagcg gcgacacagt ggacatgctt gttgaaagtc
    29881 gcaagatcgc cttgctgatc atcatgcaaa ccctattcag caaggatgtg tgggccgacc
    29941 tgccccgcat ctggaagccg atcctcaaag ccatagactt catctcgccc ggggcatgga
    30001 tcatctggcg gaatatgccg cgatttgggt atcgcaagcc gctgcgcgaa ttggatgatt
    30061 acttgtttga gatcattgaa gaaaggcgaa agacgaaaca agcgccccat gacctgctcc
    30121 agcacttaat cgacgcaggc ctgaccgata aagtcatccg tgaccagatg ctgacaatga
    30181 tgatcgccgg gcacgataca tccaccgctt tgctggcatg gaccttcgcg ctactgggtc
    30241 agcaccccga aacgctcaaa catgttcttg cggaggtcca ttcaaaagac aggtcgccgc
    30301 tgctcgacca tgtcatcaag gaaagcttac ggctgtaccc acccattcac atcggcaacc
    30361 gccgcgtggc aaaaaccatg gaattcgcag aaggcacagt ccctgctggc gaaaggatgt
    30421 tttactccat ctacctgacc catcgcgacc catccatctg ggaaaacgcc gaagaattcc
    30481 aacccgaacg cttcgcccac ggaagaaaaa caccaccgat gagttacatc cccttcggcg
    30541 gcggtccacg ggcctgcatc ggtgcagcgt ttggccaggc cgaagcacgg atcgtactga
    30601 ccagactgtt gcagaccttt tcatttgaac cgatgaaccc agataagatc cacgcgcaca
    30661 tgggagccac tcttgagccg cgcccaggcg tattgatgaa agtacaaccc ctaattccat
    30721 gacctacttc ggtttccttt tacgcttcct cgtcatcccc atcctcatct tcctcgcaat
    30781 cacatggtgg gacaacaaga acaaccgccc cacactgggg ttcaccaatg gccgcgcagt
    30841 gtggatcgca atccttgttc acatccttct ggcggtaacc tacaccacgc cgtgggacaa
    30901 ctacctcgtt gccacaggcg tatggtttta caaccccaac ctcgtcacag gcatcgtgtt
    30961 tggttacgtt cccatcgagg aatatacttt ttttgtgctt gaaaccatcc tctccgggct
    31021 gtggtggtgg ttcctcgccc gcagaatttc ggagccaggt ggcttcaagg catccatgcc
    31081 agcgcgggtt acttcgtcca ttgttttgtt cattgcatgg gtgatcttta cagcgatctt
    31141 tttcagcaac aacgagccgc tcacctatct ttccattacc ctcttttggg cattccctgc
    31201 catccttccg caattgctct acggcgcaga tattttgtgg caccaccgca cgctggtttt
    31261 gctcggcatc cttatccccg gcacctatct ttcgctgatg gatatcatcg cgctcaacga
    31321 aaccacctgg tccatttcaa aggcgcaaac cacagggatc ttatttttcg gcatcctgcc
    31381 ccttgaagag gtggttttct ttttcatcac caacatcctc atcaccttcg gtatgacact
    31441 tttgctgtcc aacattggca ggcaaaggtt cagcgattgg aaactcaaca actacaaagg
    31501 cctcccatga aactcgaaca attcaaaaaa caacaatttc tcaacatcga atccttccgc
    31561 aaaaacggac aggccgtcaa aaccccggtc tggttcgtgc aggatggaga aacgcttctt
    31621 gtctggaccc aggctggatc tggcaaagcc aaacggattc gcagggatgg aaagatccgc
    31681 attgcgccat gcacagggtc gggtgaactt ctcggtgaat gggcccctgc atacgccaaa
    31741 acgaacgaat ccgttgaaac agtcaagcac acgaccactt tgatgaagaa aaaatacggt
    31801 ctgttcttca ccatcttcgg cttcatcggc agaatgcgcg gcggttcaaa ctacaccacg
    31861 atcagtattc aactgcaaaa ccagtgagac agtcacaggt catgcaccaa caaaaagagg
    31921 cagctgcgag gctgcctctt tttacttttt cgtacgctct actttcctct accccatgtt
    31981 cttcttcatt acccgcttca atgcgaccct gtaaatctcg gtcagttttt cgcccatggc
    32041 tttggcttca gggttatccg caccttgcga gtccatctgg gcaaccagtc cgctcaaggc
    32101 atcgatgaac tgctgattaa ccatctcttc gttctgtgag agcatcgcat ccaacgtggc
    32161 ttcatcagga gcctggagca aatgctcaat gaacgcaacc tctggcggag tgctggcagc
    32221 ctgcaaaacc tgcaccatct tttgtaattt acccatgcgg gcatagtcat tcttttcaga
    32281 agcctgcctc aacaactgat tgaccacctc cactgcgtcc tgggtgaagt tctcaaggtt
    32341 ttcctgtgtg gccctttcaa tatcttcctg ggccagcaaa ttctctacaa aatcctgggc
    32401 ctgtttgtaa cgggcctcca tttgcttgtc cacatcgctg acgaagccca gcactttatc
    32461 gcgaatgcct tccatacggg acttttcttc accagccgct ttttcgattt tctcggtgag
    32521 caactgaaag aagacataat ccatgccgcc gcgagccagc gaaacatagg cgcggatacg
    32581 ggaatcattt tgcgattcga ggacaaaatc cagcagcttt tcgcgggtca aaccctgacc
    32641 tgcatcctgc aatgtctttt gggcagcttc caactctgca acagaatcac gcaactgacg
    32701 gccaaaggcg gtttcatcca acaattgacg ctgcaggtcg atcaatgcgc gagcaatggt
    32761 ttcctgtccg ctttgcatgg cgttttgggc gatgcggctg aaaagcgaga agaattgttc
    32821 gtcgatcgcg ttttcgttct ggcggatcat ctcaaggcgg gcatctgccg aagtcatgcg
    32881 aatcaacttt tcgatcagca tgacccgttc ctgctgggtc ttgatcatct ccgatgaaat
    32941 tccatccttg ttgaggatgg tttcgatcat cgattcatag gtcaggttgg cttgcggttt
    33001 gagcaggtat gccttgcgct tttcaagcgg caaacgctct gtcacctgct tgatcaatgg
    33061 accgatcaat ttctcctgct cgttgacagg taaccccaac tcaaatggga aaaaagtgag
    33121 caggagttcc ttgtcattgt cgtgatagac aacgggtgtg gcaaggcggc cttcatatcc
    33181 gcaatactga cagcgggcat ggttcgactg tccgctcagc aggcgctgtt tggcggcggg
    33241 ttcgtgcgtc acatcgaaca actgctcgac attggcggga atcatctgtt tacaacgagg
    33301 acaagcaatg gtggtttgag gcatttacga tttccgattt acgattttgg atttacgatc
    33361 aacattcgat cgttcgatat tcggaaaaag caggccgaac atcgaacgat ccattttttg
    33421 aagttcttta tcccagcagt tcttccaaac gattgatgta gccttccatc acggcgaagg
    33481 tctcttccac aggtttgggg gtggtcaggt ctacgcctgc attcttcaag acattgagcg
    33541 agtaatctga attgccagcc ttgaggaagc cgaggtagtc ttccacggcg ttgggttcct
    33601 tgcgcaaaat gcggcctgcc aaggcatgag cacctgaaat ccctgtagcg taggcataca
    33661 cgtaataatc tgcaaagaga tgcgggaagg tggcccaggt catgccgacg cgttcgcggt
    33721 ccatgtcaaa gtgcggacca aacccctcgg cgaacagatc agccatcagt tcgatcatgg
    33781 aatcggcggt caacggttca ccgcgttcgg cgcgctggtg cgtctccagt tcgaaacggg
    33841 ccagagtcgg catttggaag aaatagcgga acatgttgct gcccaccgct tcaaggatca
    33901 acgcgacctg gaagtttttg tctttcacgg tgttgagcaa atgtccgcgc agcaaagcct
    33961 gattgaaatt ggaggctact tcagccacga acagcgaata atcggtgttg atgaactgct
    34021 ggttcttgaa ggtgtaatag gtatgcatcg agtgaccgag ttcatgcgca agcgtgctca
    34081 tgctgaccat ttcatcggtg taactcatca tgataaaggg atgcgtacca tacgaacccg
    34141 aggaaaatgc gccatgcccc ttgccctgat tcgggtacac atccacccag cgttcggtgg
    34201 tgcaggcacg ccgtacctga gcagtgtatt ccttgccgag cggggcaagg ccttcacaga
    34261 tcatatccac ggctttgtcg taggggatct tcaccttctt cttttcgatg ggcgcccaga
    34321 tgtcgtaata tgcgaatttc ttcaaaccca gcgccttgcg gcggatattg aaataacgat
    34381 gccagacagg gatgtttttc ttgaaagtgt cgataaggtt gtgaaagaca tccgtgctga
    34441 tgttgagatc gaacagcgac gctgaaagcg tggattcgtg ttttcgcaca cgcatattga
    34501 acacgttgga ttgaatatta tgcgcgaggt tgctggccag cgtgtttttg tgcgccacat
    34561 attgatccat gtaggtaatg taagcagcct tacgcgcttt gcggtccgca agccccatca
    34621 attgcgtatc gtaattcccc tgtatcacat cgaaggtttt gcctgcgcta tccttgatgg
    34681 atttgaattt gaaatcggca ttggtcaaca tactggtgga gttataaaaa ccaccaaggg
    34741 gctcgcttac catgccaagc acttcttcca cttctgccga gcgcacgtgc gcctgcttgc
    34801 ggaaaaggtt ttcgacatag aacttgtaaa cgccaagttt ttcttcctgc tgaaagaatt
    34861 ggtcaagttt gtctttaccg atctcaagca actcaggctg aaggaacgaa actgccgagg
    34921 ccacctgtcc gtacataccg cccgccttgg cacgcatccc tgccgcggtt tgatcggtgg
    34981 tgttcaccga ataggaaaag ctcgcataca tgtaagcctt ttgagcgcgg atcaggatcg
    35041 cctcgaaagc ctgaaaacct gcaagcaaag cggcggcact ttcgcccaaa ccgccttgat
    35101 gtttcttcac ctcggggata tctgcaataa tctgctcaac ttctttttcc caggctttat
    35161 cacttgggaa aacactttca gaattccatt tatacttttt atcgatttta ctgcgtggag
    35221 ggatggaagt agccatgggg tatccttttc atcaatcaat tatttttcga ttttaccatt
    35281 gcaagcacaa cacttaattt cattatcgag gcagggagaa acaaatcctc gcaccagatt
    35341 cagattttgc cgagcccgtc acagcatccg cccaaatatg cccaccatgc gcttccacga
    35401 tggcacgcgc aaggtaaagc ccaaggcccg ccccctttgt ttgttttacg gcatttgtcg
    35461 aacgataaaa acggtcaaag atgtgaggca ggtctttggc ttcaatccct gggccttcat
    35521 cgcttacaca aacgatcacc tgctcgggca atacactccc gctgatcttg atctcaccct
    35581 tcggcgcata tttcaacgaa ttggaaacca ggtttgagat cacctgctcc aaacgatttt
    35641 catcccccaa aataacgggg aaattcgacg ggaaatcggt gacaatgcta tgcttcggag
    35701 attgcgacgc aaaccgctcg ctcaccctta acgcaaggct gggtacggcg acatcagcct
    35761 gattcaagct gagaccaccc gcctgcagcc gtgaagcatc cagcaagtca tccaccatct
    35821 tcgacaaacg gtccgcctct tcttcgataa cggccagcga atcattgatg gtgtgtttat
    35881 cccagcgtgc atcatcacgc cgcaacgtgg aggcgtaacc cttgatcaaa gctaccgggg
    35941 tacgcaactc atggctgaca atggaaataa acgtcgcttt gatctcatcc gctgtgcgga
    36001 aatgggtgat gtcacgcacg ctaagaatga cgttacggag cttgccttct tcagacagca
    36061 agggagcgta ggtaattccc accggaagcg gcggtggcgc aggtttcgca agatcacctt
    36121 ctacgtacag ggttgcgttc ggtgtgagcg gccagccctc cgccaccgaa tcttcaaggg
    36181 taatgccttg cggtgtcttt ttccacttga tgatatctgc atggtttttc ccagtgatct
    36241 cctcacgagg ttgaccaaat aatttttcaa aagcgccgtt acaacgttcg atgtcgaggt
    36301 ttgcatccaa aatcaaaatg ccatctgccg ctgattcgag taatgcatcc agccttttct
    36361 tttcataaga aacctgaccg tacaactgcg cgttatacac ggcgatggca gcctgatcgg
    36421 caaaggattg caagagaacg cggtcatcgg gggtaaacaa gtcggcgtaa ttgcggaaga
    36481 taaagatcac accaatgacc tgtccatgtg cggcaagggg cagaccagtg ccgtttaata
    36541 accccatgct ggcagtatag gtcagatttt tcaacatgcg gttcagttca cgcacgtcca
    36601 attcgcgaac tttttcttcg gcaagcaacg gggttaagta ggagagaaat gccggggcaa
    36661 tgccatgtgc cgcgctgacg cgccagccgt cggcttgttt gagagcgatc agtccagcct
    36721 gcccggccaa catctcaatc gaaacgcgca aaattcgcgc cagcaacttt tcaaggtcta
    36781 gttcttcagt gatcaggcgc gaaatttcca gcaaataatc gcgttgacgg acgcgaaaat
    36841 cgggaagcat cgaaggaata atggacattg ctgttatttt atcactgcgc tacggcaaaa
    36901 ggcaaaaaca gccaaaccca cctctggata acatatttgt tttgcttgca ttaatgaagg
    36961 gaaataacat tgcaagtaag ttaacatggt tgtaaaatga tgagaaatta atagtactta
    37021 aggctaattt cagatatcct tcatgtaaat tgttgcaatt ttcagccttc attctgattt
    37081 tgcaggagca aatgatgaga attttttcaa tccaagcgtt caatcaatcc aaatctgcca
    37141 tggttcgcct gttcgttttt gttttgcttt tggcgtccat gctggcacag gagcatgtgc
    37201 cgggggtaca ggctcaggcg gggacacccc aactcatttt gaccaaaacc attgagggtg
    37261 gcgcaaccac ggccagcctt ggcgatacga tccggtatcg catccgtttt gagtgcagca
    37321 gtctgacaac ctcctgcgga caaatggaaa ttaccgatgt gctccctgcc gggctgacgt
    37381 atgccccacc cccggcttct tcagcaccga ccggtttcac cattacagaa gccccggctg
    37441 gaacgattac cattacaaaa gatgacaaca acctgctcga tggtacccag tacgatgtgg
    37501 tcatcgccgc aacgattgat tatgatcttc gtcctcttcc tgccaccatc aacaacacag
    37561 tcaatggacg ggtggatccg cccggcgccg tcagctggca aaacgcaact cctgcaagcg
    37621 cacctcccat cactgtgagc aatgtcggtg aatattggga tctgacaaag accttgtata
    37681 gtccgtccgt caaccccacc atcaatacag atgttaccta tcgcatccaa ctctgtccaa
    37741 ccacaccgcc gcccggcacc agcaacgtgg cgcttgaaaa cattacgatc acagataccc
    37801 ttcccacagg agcaacgttt gtctctgcca gcgatgcagg cacagaggca gccggtgttg
    37861 taacctggcc gctcattgcg gggcctgtct tcccacccac ctgtgttacc cgttatgtca
    37921 caattcgata taacagccca acctttgcag taggcaacaa tgttacgaac acagccaacg
    37981 ccagcggcac atacacggat tcaggcggtg gtacgatagg accaatcagc atcgccactg
    38041 acccaatcac ccatccgatt gccaccgtgc tggaagttcc cacttacagc aagaatgatc
    38101 aaggggatcc agtgggtatc actggaactg cccggtttgt gttgacgctc aacacaaacg
    38161 gcacgaactc gccagccaat gatctgattc tcattgatac actgcctcct gaattacagg
    38221 ttacttcagt gaccagcggt cagtggaatg ctgcatttaa ttatgttcgg gcttatgtgg
    38281 aatactccac caactatggc agcagttata cagcttttcc gggccagccc atcagctaca
    38341 acagtaatgc cacatacact gcgcccgctg ccaacataac caacgttcgc tggcgttttg
    38401 aatacgatcc cgatgcggcg gcacctttct tatttacaca ggctggactt cccttcgctt
    38461 gggcgtttac caccagcccg caaatccgtg taaccccgcg tgcaacggca acaaccgcag
    38521 atgctccttc gggcgtagcc atgcctgcag ctgttgcagg gacacgctat gaaaactgtc
    38581 tgcaaaccag ccgtaccagc aatgcaggtg cccccgtaac tgacccatgc gatattgaag
    38641 ggatgacagt tcaaggcaca ttggccagtt tgcgtgcatc gaaggctgag acccgcggca
    38701 ccagttggga tgctgataac gatccgctca ttaatacatt taccagtgac agcactttat
    38761 tgccaggtga taccctgcgc tataccatca cggtcagtgt tactgagcgt tcatctcagg
    38821 cgatgatcga cccaaccatt cttgatacga ttcccgctga cttgatcttc gtccgcaatg
    38881 gaacggcatc agtcaacggt accggttgtc cagcacttgc cagtcagccg actttcaccc
    38941 aagcaggcac acaactgaaa tgggacttca caggtttctc gctcgatcat caacccagcg
    39001 gacttggcag ctgcaacctg gtcgttgagt tctatgcccg catccccgcc ggacaaaccc
    39061 caggtacccg caccaatgac ctgatccttg tcaccgactc ggatgacatc gtatgcgaaa
    39121 acggcacaga gatcgttgac actacagata tcgatagcga tgccaacaac accgagcccg
    39181 cctgtcaaaa tccagatact tatgtggtgg agcgttcagc tgcattacgc ggtgaaaaat
    39241 ggattcgtag tgtcgatcct gaaaacagtg tagtggtcga ctctaccacc ttccaaccca
    39301 gtgcctcttg tccggatggc ggcactgtag gtttggctgg aggcggcagc aaccccttca
    39361 ccagattccc ctgtatctca caggcattcc ctgaaggagc tttaagcatc ggtcaacgcg
    39421 ttccaccgcc gcttaactct gccctggatg atttcgaata caacttgcgc atctacaacg
    39481 atggcaatgt cgaaatgtta agttacgccc tgtatgatat tttcccctat tatggcgaca
    39541 ccggctccgg cggtactttg gccaattccc cacgcctctc agaattccgc ccgatcctgc
    39601 gtggacctgt ggaatttatc agcggtccaa gcctaagcag caccgacttc accatcgaat
    39661 ataacaacac caccaatccc tgccgtccgg aagtgttcaa tcagcctgca ggcactccta
    39721 atatcccagc cgggtgtaac aacacctgga gttccacctg gtcggcagat gcacgctcat
    39781 tccgcatttt gctgaattcc ccggccgtca tcccgccatc tgccaccacc tccgaagtac
    39841 gcttcggcat tcccatgtac attcccaagg atgcgcccac agtcggcgta tttgacaaca
    39901 acgacccgtt gtcacttgaa attgcatgga attcattctc acacgtcggt tcttacgaca
    39961 gcgaccccaa tgtcccagtc gtcattcaag acttgctggc atctgaaccg cgcaaggtcg
    40021 gtatcaccat tcccgaacgc atgagcgttg gtaaccgcgt ctggcgtgac tcggataaca
    40081 gcggtcacat cgatgcccca gatgatatcg cccccggtat tgcaggggta acggttaacc
    40141 tataccgtga tgtggatgat gatggcgtac ccgacgggcc agccattgcc accaccacta
    40201 cggattctct cggctactat ctgttcagca atattcctca tgatctggca gatgttaata
    40261 acaaccgcta ccttattggc attccatcaa gcaattttgg atccgggcag ccattgttca
    40321 acttgcgcag cagcacaggt gtgttcgcta caacagatga cagtgatgac aatggcattg
    40381 accccgtcac tgccgggcag gaagttttca gcgccagttt catcctgcaa cctaccaccg
    40441 aagtcaccgg agaaaccgat ctttcatcca acgacctcga tggtcccccc ggagaacgtc
    40501 gtggcaccaa tgcagaacgt gactacaaca gcgaccttgc cattgacttt ggtttcttcg
    40561 gcggtactga tgtacctttc agcatcggca atcatgtctg gttcgataat gggcgcactg
    40621 gcgcatccac tttcaactat gccttgaaca atgacggcat ccgccaggca acagaactgc
    40681 cggtcgtcgg gttggatatt cgcctatatc gtgacggcaa tggcaataac actcccgaac
    40741 ctgcggaaat gattcgcacc gatataaccg acgccaacgg cttttacctt ttcgataacc
    40801 tcgaccctgg accctacatt gtggaaattc cagccaatga attcggtgct ggtcagcctc
    40861 tcgaaggctg gaacaacagt caggtaatag gaaccgagac aaccggtgtg ccgggcaata
    40921 ccaatattcc aaacgtggat agcgatgata atggtgtcga ttccagcact ccgcatgttg
    40981 atggaatctt cagtggtgtc atcgtcctca cacgcggcac caacgaaacg actggtgaaa
    41041 ctcatatcag caatcagcca gacccggatg gcaannnnnn nnnnnnnnnn nnnnnnnnnn
    41101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41161 nnnnnnnnnn nnnnccaacg aaacgactgg tgaaactcat atcagcaatc agccagaccc
    41221 ggatggcaat gccgccacgc ccaaccttgg catcaacccc accgaatggg atggacctgc
    41281 ttcacgtgga cgttacggcg aaagcgacac aaccagcaac ctaaccattg actttggctt
    41341 cttcgaagta tattcactcg gtaatgtagt ttgggtggat gatggaacgg gcggtggcac
    41401 tgcagatgat ggcctgcgca atggtaccga acccggcctg aatggcgtca ccgtcaactt
    41461 atatctggat gcgaacaacg acggacgccc agacactgtt acgcccaagg caaccaccac
    41521 aaccgccaat ggcggatatt accgcttcga caaccttgcg gctgatacct atctcgttga
    41581 aatcgtccta cccgctggat attcgagcag cccaatcgat gcaggcgacc ccgataccga
    41641 cgaagatgat tttgacgaca atggtgtggt tccggccacg atctcaactg ttctcagcaa
    41701 cccggtcacc ctcgggcgtg gaacagatga acccacggtt gatagtgacg cggccacagc
    41761 caataatcct gaacctggtg aagcacccaa ccaccactcg aatcgcaccg tagactttgc
    41821 cctaactctt ggctacagcc tcggtaaccg tgtctgggtg gatttggacg acagcggcac
    41881 gatcaacgcc gccgatggcg ccacacccgg tcttgataac atgaccgtga acctgtatcg
    41941 ctccagcgac cccagcacca ttatcgccac caccctaacg gctggcggtg gatattatcg
    42001 cttcgataat ctgctggcag gcgattatat tgtggaagtg gtctctcctg ttggttatac
    42061 cagcagcagt gtagatgcca acgatccgaa caccgataat gacagcgatg acaacggtgt
    42121 gattatttcc gcaaccacgg tccgcagcaa ccccgtttca ctgggcggct tagaaccctc
    42181 cactgatgac gacccactgc ccaacccagc cgccacaggt gaggccccga acacccgctc
    42241 caaccgcacc gttgactttg gcttcttctt tgtgccttat tccctcggca accgtgtctg
    42301 ggatgatagc gatggtgcag gcggcacacc tgacaacggt gttcgcgatg gcgctgaacc
    42361 cggcattgat ggcgtcacag tcaacctgta tcgcgactct gacaataaca acaccccaga
    42421 tggggcaatt attgccacag atacaaccag cggcggtggt ttctatcgct ttgacaacct
    42481 ggctgcggac acctatattg tggaagtgat tccgcccgcc ggttatgcca gcacacttga
    42541 tgcaggcgac ccggacacag acctcgatga cgatgatgac aacggtgtcg tatttgcagg
    42601 tgcgaacatc cgcagcaacc ctgtacggct tggcccgccc gaacccagcg aaccgactct
    42661 cgaaaacaac ccggcggtca acccagaaac cgccgatggc gaagctgcag acgatcactc
    42721 caaccgaact gtagactttg gctttgtgtt gggctacagc ctaggcaacc gtgtatggga
    42781 tgatagcaac ggcgcaggcg gtacacctga gaacggcctc cgtgatggta cagaacccgg
    42841 tatggatggc gtcactgtca atctttaccg tgactcgaac gacgacagca accccgatgg
    42901 cggcattatc atgtcaacct ccacctcggg aggaggcttc taccgtttcg ataacctgat
    42961 ggctgatacc tatatcgttg aagttgtcat ccccacaggc tataccagca cgcttgatgc
    43021 aggtgacccg gatacggaca tagatgacga cgataatggt gtggtcatcg ttggttccaa
    43081 tgtccgcgcc aacccggtca cgctgggtcc tgccgctgtt ccggagccta ctctcgaaaa
    43141 taacccggct accaacccag tagctggtga atcaccggat gcgcgttcca accgcaccgt
    43201 tgacttcggc ttcatccgct ccagcactcc cccgccgcca accggttcag ggattcagat
    43261 cgtcaagacc gccagcgcga gtgttgtgaa gccgggtgat actgttgtgt attcctatgt
    43321 tgtttccaat actggcacca ccccgcttag aaatgtcatc gttaccgacg acaaatgctc
    43381 ccccgttacc tacataagcg gagatgtcaa cgcgaacaac atcctcgaaa cgaccgaaaa
    43441 gtggaattac acctgctccg ctattttgag cgaaacgacg accaacatcg ccacggtaac
    43501 cggtaaagac ccaacgggcg gcgatgtcaa agacacagac acagtcaccg ttacggtcga
    43561 cacgaatggt ttctccaaga tcatcaccgg caccagcgaa accttcacca acggcatgga
    43621 tgtcgccatt ggcgagatcg tcacctacaa gatcaccatg agcctgcccg ccggactgcc
    43681 ctacaacaat gtggttgtca ccgaccgcat ggacaagggt cttgcttttg tagactgcgt
    43741 atcggccacc ctgggcggcg tggatattct caaccagatc tgcccgccgt tggtctctgc
    43801 gatcaaagac ccaattgacc cagccagcaa cccagccaac ccgggccgtc aggcacaatt
    43861 caatatcggc aatgtatctg cttccacaaa gccgacggca ttggtcatca cataccgtgc
    43921 catcgtgctt gacgtcatcg aaaaccagga aaaggatacc ttgaacaaca aggccaccct
    43981 cacatgggat ggaggctccc tcactgccag cgcgcctgag gttgtcatcc tcgaacctga
    44041 ccttgatatt gagaaagctg ccaacccaac cacggtcaca gcaggctcat ccgtcgagtt
    44101 caccatcttc atcacgcaca cttccttgag cctcacggat gcattcgatg tggtggtaac
    44161 cgacgttctg ccgaaaggcc ttgaatacgt ggcatgtacc cccatcacct atgagggcgt
    44221 cgttccaacc aaacaacctg acccatgcag catccccaat ggagacaccc tgcaatttga
    44281 atgggatgtg ctcccgctcg gtggaaactc cgtcatcaag ttctttgccc gttatgatgg
    44341 ctcgcaagtc agtgtcatga acagtgccgc agttgcatgg acctccctgc ccattgatta
    44401 catcaacggc aagccagtcc aacactctgg gtacaacgac acatctactg aacgctggta
    44461 cgacccgaac gacctggtca acatctacca gatcagcgac aacgtctgga tcaacccgcc
    44521 gaccattacc gaaccaatcg aggaagagga actaccgggc agccttcctg ctacaggctt
    44581 cgccccgaac gtcatcaccg ccctgccgca gcaacctgcc aacaaggcct acacaaccac
    44641 cgatatctgg gtggagattc caagcattgg cgtaaacatg ccgatcgtag gcgttccgct
    44701 cgttgacggt gattgggacc tctcctggct gtggaatgaa gcaggctggc tcaatggcac
    44761 ggccttcccc ggatgggatg gcaacagcgc cctcaccggt cacatcaccc tgcccaacgg
    44821 cagacctggg ccatttgcca tgctgggcaa cctcaaatgg ggcgataaga tcatcgtcca
    44881 tgcctatggc acggcctaca cctacgaagt ccgcgagact cgtacggtca gccccgacaa
    44941 tatgacaatc ctcaaacacg aagaggaatc ctggctgacg ttgatcacct gtaagactta
    45001 cgacaaagcc accaacacct atgcccgccg tactgcagtc cgcgctgtgc tcatcagcac
    45061 caccgccgac aagcaaacca aggaagccaa aagcggacgc tagaaactaa cccaaacaaa
    45121 aacttccggg ccaacacccg gaagtttttg ttttaagcac attccactta ctttctactt
    45181 catttccttc tcagccaccg tcaatgcatc ttcaaatatc ttcaaacctt cttccatttc
    45241 agatttactg atggaaagcg gaggcgcaat gcgaatgacg ctcttgccgc aggaaagcat
    45301 caacaagccg cgttcgaaac cgagatcaac aacacgctct gtcaaatcat ggtcgggttc
    45361 ttttgtttcg cggtccttca caaactccac accgatcatc aagccttttc cacgcacatc
    45421 gccaatactg gggtgacgtg atttaatctc ttccagggca tccaacgcat agctccctac
    45481 ctcttcggca ttcttcatga acccattttc gatcaggtca atcgttgcaa gagatgcggc
    45541 acaagaaatg ggattgccgc catacgtgtt tccatgtgtg ccaatttccc aatccatcac
    45601 agacttgcgg gcgatacaag cgcccaaagg cataccagaa gcaatgcctt tggcagaagt
    45661 gatcatatcg ggctccacgc caaaatgctc aatagcccac cacttgcctg tgcgtcccat
    45721 accagactgc acttcgtcaa ggatcatcag aatgccatgc ttgtcacaca gcttcctcaa
    45781 agcggggtaa aaaccttcgg gtggaatgat gtagccgccc tcgccttgaa tggtttccac
    45841 caaaattcca gccacatcct tgggcggaag aatttgggca aggatctcct cctcaatata
    45901 gcggacgacg gcttcgccat aatcttcgcc tttacggcgt tccaacaccg ggcgatacgg
    45961 gtttggaaac ggcgcatggg taacaccatt catcaacgga taaaacccac cgtggtattt
    46021 cgctttgctg gcggtaaaag tcaccgctcc catggtacgg ccgtggaacg cgccggtaaa
    46081 accgatgaaa ttcgtacgct tggtatggta acgtgccaat ttgatcgctg cctctaccgc
    46141 ctcagtgcct gagttggtca tgaaggtcag cgcatcttcc ttgaacggtg caatatcgct
    46201 caatttttca ccaagccgaa tccaattttc gtgataaaaa tccgatgaaa tatgaatgaa
    46261 tttctcagcc tgctcttgaa tggccttcac tactttgggg tgtgagtatc ctgttgaaac
    46321 cacggcaata ccgcccataa aatccaaaaa gcgattgcca tccacatccc atacttctgt
    46381 tccttttcca tgatccatta caaatggata tccacgcggg tatgaaggtg atattacctt
    46441 tcgatcccgc tcgatcattg ccttggcctt tggcccgggt aaattagggt ttgtcataca
    46501 ttctccttgt ttcaattaag ccgctttcaa ggcgcacaga ttcaggcgac cccgctgggt
    46561 attgaaaact tgtgcacctg aaaagcggtc ttatttatga acttgtaact cagctacagc
    46621 cgagtcgtcc cccggtaaat ccatgtggat taagaaccgc ctctcactca ccgaaaattg
    46681 ttcagccgag aaaaaaacat ccttacgcct cagcaactac cggcgcaggg tgaatcgttg
    46741 atttcatcca gattctcatt ttttcgggaa aattcttcca ataaaaggtc aaaaatcgac
    46801 cttccaccaa ggaatatggc aattataccg tatgccctgt ttgccgcctt ttccgccgtt
    46861 gcaaccccat gcatcaaaga tttgactcct catttcaacc ctgctattat ccttttatgc
    46921 gaaaaaattt ccttgctgag ttttggcagg ctctacgcga cattccgcgc tggctgaggc
    46981 cggggttggg cataaaacgc tggcttaccc tggtgctgat cggaatcact ctgttggggt
    47041 tggggctggc agtcattctt attgacctgt atcgcacaga ctcggaaaac ccgatcctgc
    47101 tgacctttct ctcctatgcc tcgttgcgct tcattccgcg agtgtggcgt gccgtcattt
    47161 ttggcgggtt gggtgtgggc gttgtgatct atggcatcgt gcaactcaac cgttccctgt
    47221 tgacgccatt cgtccgccct ggcgcggatg tgtacggtca gcttaccgac tttcgccgtc
    47281 gcgaacgcgg tccacgcatt gtcaccatcg gtggcgggca tggacttgcc acacttttac
    47341 gtgggctaaa gactcacacc cgaaacctga ctgcagttgt gactgttgca gacgatggcg
    47401 ggtcatctgg caggttgcgc gaaagccatg gcattttgcc gccgggtgat attcgaaact
    47461 gtctcgccgc cctttccaat gacgaggcca tgttaaccca gctttttcaa tatcggttca
    47521 gcggctcgcc cggtttggaa ggtcattcat ttgggaacct cttcatcaca gccatggctg
    47581 atattaccgg tagctttgag gaagcggtgg cagaatcggg ccgtgtgctt tcggtaaatg
    47641 gccgcgttct gccttctaca ttgcacgatg taaaactggt cgccagcatg gaactccccc
    47701 actcactcaa cgaaatccgt gttgagggtg aaagcaaaat tccaaccatg gcaggacgag
    47761 tccgtcgtgt gtggctcgaa ccagataacg ctcctgcatt cccacctgtt atcaaatcca
    47821 ttctcaatgc cgatgtgatc gtggtggggc caggcagtct ttttaccagc attctcccca
    47881 acctgctggt taatgacctg ctggccgcca tgcatgccag ccgagcgata aaaatatacg
    47941 tctgcaatat cgccacacaa acaggtgaaa ccgactcata cacctgcttt gatcatgtcc
    48001 gcgcactcga gggacatgtg ggcgaggatc tgttcgatgt cattttgtgc aacgataact
    48061 accgcggcat aatcagcgct gactcaaagt gggtattggc cgatgaaaag atattatctg
    48121 attctcgcac atactgtgct gatcttgtag atgacgatca cccatggcgg catgactctg
    48181 aaaaacttgc ccgcgaaatc atacaaatct tctctgaaaa gactggacca ttggtctagt
    48241 caagagtgct ttccccaaaa agtaaacgat tacattcgtc ctattatttt tggacgtacg
    48301 aaaattcaca aattctgctt aaagcagtaa aattaaggct gtatgaaggc tcataccaga
    48361 gcaggcacaa attttaggat agatgtgtcc tctgttgtaa caaaaccaaa ttggaggatt
    48421 ttctcatgac agtaaaaatt ggcattaatg gctttggccg tatcggtcgc caggtactca
    48481 aggcaatccg cgatatgtat ccaaacgaat tggaagtcgt tgcctttaac gacattggcg
    48541 ataccaagac catggctcac ctgctcaagt acgactcaac ctatggccgc ttcaacggtt
    48601 ccgttgagat cgccgacgat ggtctcctga tcgatggaaa aaaggtcaag gtcttcaaag
    48661 agactgaccc cgctgccatc aaatggagcg agctcggcat cgacattgtg atcgaatcaa
    48721 ctggcttgtt caccatcaag gatgacggtg tcaacaagaa gggtaagacc gtgaagggtg
    48781 ccgtcaatca catcacctct ggcggcgcga agaaagtcat catctcagca cctgccgaag
    48841 gtgaagattt gaccgttgta ttgggcgtaa acgaagacaa atatgacccc gccaaacacc
    48901 atgtggtttc gaatgcctcc tgcaccacaa actgtctcgc gcccgccgcg aaagttgtgc
    48961 atgacaaact caatatcaag cgcgctttca tgaccaccgt acattcctac accaacgatc
    49021 aaaaagtgct cgatatgccg cactcggatc tgcgccgcgc ccgcgcctca ggcttgaata
    49081 tcatcccgac caccactggc gccgccaaag ctgtcgctct ggtcatcccc gacctcaagg
    49141 gcaaattcga tggttactcc ctgcgcgtcc ccacccccac cgtttcggtt gtggatttca
    49201 ccgcagaggt tgaaaaggaa accaccactg aagaactgcg ccaaatgttc cgcgatgccg
    49261 ccaaagaacc gcgcttcaag ggcatcctcg aagcggtgga cgaacccctg gtcagcatgg
    49321 acttcaaggg cagttcctac agctcatccg ttgacctgcc gttcacttcc gtcctcggca
    49381 aagaaaagaa caacttcatc aaggtcgtta cctggtacga taatgaatgg ggttactcct
    49441 gccgtactgc tgacctcgcg gcgatgatgg caaaaacact gtaaatattt tgggtaggga
    49501 cacggcgatg ctgtgtccct acaaaattaa aatgaacaag aaaaccgtaa aagatattga
    49561 tcttaagaac aaacgcgtac tcatgcgcgt ggatttcaat gtgccgatgg cagatggaaa
    49621 agtcaccgac gacaagcgca tcaaagccgc actgccgacc attaaatatg ttttggaaca
    49681 gggtgcttcc ctgcttttga tgagtcattt gggacgccca aaaggcggtc cagaccctga
    49741 gtttagcctg cgtgctgctt cggaagcatt atcggcactg ttggagaaac ccgtgcaaat
    49801 ggcgcccgat tgcgtcggcg cggatgttga agcaatggca aaaggattga agtcgggcga
    49861 tgtgctcatg cttgaaaaca cccgcttcca caagggcgaa gaaaagaacg atctcgacct
    49921 tgccaagcag atggctgccc tgggtgacgt gtacgtcaac gatgcgtttg gatctgctca
    49981 ccgtgcccac tcctccaccg aagggattgc ccgcttcctg cccgccgtat ctggtttttt
    50041 gatggaacag gaacttgaat acctgggccg cgctgttgcc aaccccgaac acccttacat
    50101 cgccattctc ggcggtgcga agatcagcga caaaatcctt gtggtggaaa cccttgcctc
    50161 aaaatgtgac aagttgatca tcggcggcgg tatggcaaac accttcctcg ctgcgaaggg
    50221 attaaacatg caggacagcc tcgttgaagc ggaatcgctc gaaaccgcca aatccctgct
    50281 tggtaaattg ggtgaaaaac tcgtcctgcc tgtggatgcg gtcatcgctg ataaattcga
    50341 tgctgaagcc aacacacagg tagtggatgt ggacaagatc cccgcaggct ggcgcatgct
    50401 ggatgtaggg ccgaaaacca tcagcctgta tcaggccaca ttgaacggga gcaaactcat
    50461 cgtttggaat ggacccgttg gcgtcttcga aatgccgaaa ttcgccgaag gcacattcgc
    50521 ccttgcgcgc atgctggcag aatccaaagc cactacggtc attggcggag gtgactcggc
    50581 cagtgcagtc aagaaggctg gcgttgccaa acagatgact catgtttcca caggcggcgg
    50641 cgcctcgctg gaattccttg aaggaaaaga gttgcccggc gtggcggcac tgctcgataa
    50701 ataattttca aaaggatggg cgatttcgcc catccttttg atttaccaga accttccact
    50761 tcttttttaa aacgcaaata aaacagaatt ccacatttca gtttacaaac aatttacagg
    50821 ggtacaacct aaattaatga cactcgacta taatctaaaa aaatgcaatc ggagatagtg
    50881 tatgtcgttc aacatcggtg aaaacgtcgg cccatatcga attcttgaac aacttgggca
    50941 gggtggtatg gcaacagtat tcaaggccta tcacgcctcg cttgatcggt atgtggcttt
    51001 aaaagtttta caccctgcat tcaaccagga acagtctttc atttcacgct ttcaacgcga
    51061 agcgcgggtg gtggcaaaac tcgaacatcc aaacattgtc ccgatttacg attattccga
    51121 acatgaggca cgcccgtacc tcgtgatgaa atacattgag ggggatacac tgaaggcgcg
    51181 gttgaacaaa ggtccgcttt cagcaggtga gattgaaaca gtcgtgaatg cgattggagc
    51241 ggcgctggcc tacgcacaca ggcaaggcat tctgcatcgt gatgtcaaac catccaatgt
    51301 gatgctggcc acagatggat tgatctatct cgctgatttt ggccttgctc gcattgcaca
    51361 ggctggcgaa tcgaccatgt ctgcggactc gatcatgggg acgccacagt atatttcgcc
    51421 cgaacaggcc atgggtaaaa aagacctcga ttcagggacg gatatctact cttttggtgt
    51481 gatgctgtat gaaatggtcg tgggacaggt tccattcagt gcagataccc ccttctccat
    51541 cattcatgac catatttaca cgcccctgcc cctgccaaga aaaataaacc caaatgttcc
    51601 cgaatctgtt caacgggtgc ttctaaaggc gttggcaaaa gatcgcattg accgctatga
    51661 aaccattgaa gagctggtac aagccttcaa agacgcatgg actgaagccg gtgtccccat
    51721 gcagggcaca tccatcacca tgcactccac caatcttcgc aagcaggttc aatcaacggc
    51781 atccaaaccc aagcctgcca tgcccaacct cgtcaatgaa aaaccaagac cgcaggccaa
    51841 ggcacccgca aaaaaaggca gcgccgtgtg gatgtggctc ggcgtagccc tgactggttt
    51901 tatttgcatc atcatcgcct tcattgcaat tcgcaacaac aaccgaatcg cacccttgcc
    51961 cgttcaacct acacttgttc caactgccgt cattttagag ccgtcaccgg cgatcccaac
    52021 ccaatccgcg ccgcctacat tccctccctt aacacccccg ctcggcacag atacaactgg
    52081 tcagccgccg ctcaacccac aaggcagtga cacacccgaa atcgcagccg caagggaagt
    52141 tgtggcgaaa aatccgcagg atgcagatgc acaccttcag cttgcctttg cactttggga
    52201 tgccaaagaa attcgcccgt ccatggattc gctgatgcag gcaatgggac tggctggccc
    52261 cggtaacacg gattttctac aaaaggcagc cagcgggttt catcaacgcg aggcatgggt
    52321 ggcagctaca gccatctacc tgcgtttgca ggcaagttac gctggcgcgc ctgttcctga
    52381 agacataaaa atcaacacca acgaatcggc atacaaagca gcagaccgaa aagatttcct
    52441 gctgctcatt gcgttcgacc agatcgatgc catcgacccg cgtcttggac atgttgtccg
    52501 tgggcggcag gcattattca acaacaattt gcaggaagcc cagcttcaac tcgcagaagc
    52561 cgaaaaactc gagcccaaga tccccgaaat gtttttactc aaagctgaga tcgccctgcg
    52621 caatggtaat acctctgaag caagaaccat tttcaacaac acgctcaaca atccagccac
    52681 acccgcctgg atgcgcctga tgattgaaga gctgctcaaa accatgcaat aaggacacaa
    52741 caatgcgaaa accactcgta gctggcaatt ggaaaatgaa caaaaccatc acagaaactc
    52801 gtgacctggt cttcacaatg agcatgaaat tgcgtgaaat tgacggcgtg gaaaaagtca
    52861 tctgcccgcc gttcatgtca ctcatggctg cagccgcact gctcgaaggc tcgggcatcg
    52921 gcctcggcgc acaaaacatg cactgggaag aaaaaggcgc cttcacaggc gaggtctctc
    52981 ccgccatggt caaggagttg tgcggatacg tcatcctggg ccactcggaa cggcgctcct
    53041 attttggaga aaccgaccag atcgtgaatc ggaagctgct cgctgcccaa gccatggacc
    53101 tggtaccgat cgtttgtgtc ggcgagactt tggaacagta cgaatcaaaa cttacccgtg
    53161 aagttgtttc tactcaaacg agtcagagtc tcaggggtgt atctccatcc tatgccccgc
    53221 gcatgatcgt agcgtacgaa cctgtctggg ccattggcac aggcaaagcc tccaacgggc
    53281 ctgacgccaa tgacgttgta aaagatgtca tccgcccagc gctggcagat ttatttggaa
    53341 acgaaaccgc acaagctatc cgcgttttgt atggcgggtc ggtcactgct gcaaatgccg
    53401 ccgagttctt cggccagcct gatattgacg gcgctcttgt cggcggagcc agcttgaaga
    53461 tcgatgaatt cgtagccatc gccaaagccg ccgcaagcac cgcagcataa ccctgaagga
    53521 atccaaaaaa aattcttttc gatcatgccc ttcactggct ttctgctgtt cattctgctg
    53581 cttctgccgc tggtatttct ccagcggctt ttgcatcgtg aaattcaggc tgtcctgctc
    53641 atcctcaccc gcaactcaca gttgaccatc ggcttgtttt cggtgctgtt tttccccggt
    53701 gtgttcctgc atgaattaag tcatttcctc atggcaaagt tactgcaggt gcggacagga
    53761 aacatttcac tcatcccagc cgccctgcca aatggacgcc tgcaaatggg ttatgtggaa
    53821 accgaagaaa ccgacgtagt ccgcgattca ttgatcgggc tggccccgct cattgcaggc
    53881 tcgctcttta tcgcctacgc aggcttaaac cgtcttgggt ttggcatgct catggatctg
    53941 ctggccaatg ggcaatttgc cctgttttgg acagcactgg gttacctgcc tcaagtcgat
    54001 gatttttact tatggttcta tctcgtattt gcggtttcca gcatgatgct gccctctgaa
    54061 tcagaccgcc atgcctggct cccgttggga gtatggatag cagggctgtt tgcattggcg
    54121 atctttgctg gagcaggcca atggatgttg gaaaacctgg ccccctcatt aaacgatttc
    54181 cttggcacag tagccacttt gtttgggtta agcgttgctg ttcatgtgat ccttttattg
    54241 cccaccatgc ttctgcaccg cgccctttca aggatcacag ggttggatat aaaaaagtag
    54301 cacagacgca aattagatca agaaaaaggg aaccaccact tgggcctgct ttctttctgc
    54361 agatgagcca ctgtttcggg gtgccccagt cgaatcagca ctttctttgc catgttttgc
    54421 acaacttcac tatcatcatt tagcatggca gtaatggcat ggttgatttg gattcggttt
    54481 aacatcaaca ggtgaaccgc aggcagggca attgagcagg tcgattttca tggagtacac
    54541 cctcctacaa catgcttaac gaatgggcgg catttccgct tttttattga aagtgttcca
    54601 agccagcgaa tgcagggccc ttttttgaga cggaatggat tcggcgcgct ttagccattc
    54661 tgcttcagct tgtgcggctt cggcaaagca atgtgctgaa aattccgccc gctcttttac
    54721 agtggcgttg gcaagtctgc gcgattctga gaggaatttc ttttccattg catcgcggtc
    54781 actggcgtag gttttaatcc gttcagaata gtttagcaag gtggcacggt gcaactgttc
    54841 atgcttccaa tatgtcgacg ctgagtcgta ttttgaagca ggagcaggcc caaggttaag
    54901 agaagaagcg gtgtccatcc acatgggctt gaaaatgctg gtacaagggg cagatgtgcc
    54961 ggtgacgtaa atgagcggag ttttgtcatc caggagtaca accagggaag cggtgctttg
    55021 actgatgcgg atcgggccga aacctgcatg catgcaaaca tccatggcgg caacggtgtt
    55081 ttgcgggtcg taatgttcgt catcgtgatg acggagaatg cccatcatgg tttcgagtgt
    55141 gacgctgcct ttttgagttt caagcaggga ggtcgttgtg gcgcggcgga cacggccttt
    55201 gccgaagttg gtgtaaataa aatcggaata attttcggcg taatcaaatt gagatccaga
    55261 tttgacccag cctttttgta cggcattctc gaccagttct tttgaggcaa tatcaaactg
    55321 gccgccaagc gtaaaacaat ttgagatgga atacacatcg gtgatctgtt ttgccgccca
    55381 ttgtttatca acagtttcaa gcagccaggt ttcatttgca tctgcaatta aaaaactgtt
    55441 gtggtaatac atggtttcac catgtgatgc cgcgtttcca ccctgaccaa atttttcaag
    55501 taaatctatg atgacctgca cagcctcgcg cggtgtgatg gcacgttcca gtgcagcgcg
    55561 aagcatatcc attccgagca acgcaggctt tttattggcg ggcacttttg aatacactgc
    55621 ttcattacca atgacaagcc cgtgttcatt cacacccatt tccgcgcccc acatccaaaa
    55681 cggtttggat aatagaacgg cgtgagtctt ttcaacttgt ggaatttcaa tgtaggtgca
    55741 tttcaggctg cttcctgcgg cgtgtgattg cgcaggaaaa taaacaatat gctgaccttc
    55801 gttcggggga cggtcggaat tctttgcgaa gatggcaata ttatcggagg tggcttgttt
    55861 gctggcaatg agagtgtcgc acatggttat tgccgaagta tagcatattc attttcgttt
    55921 cagatagggt ctgtccgaat aattcaaggc gccagccgca aatgcacgac aaatgttgta
    55981 ccttgattca attcgctgat cacgtcaatt tttccgccca attcatccac aatggatttg
    56041 acgatataca ggccgatgcc cgtaccaggg atttctgcaa cggtgacgtt cttggcacga
    56101 taaaaacgct cgaacagatg cggcaccgcc tcaggaggga ttcccaaacc gtggtctgtg
    56161 atggaaatac gcgcttcatg ctccgcgccg ccgcaaactg ctgctacatc gacggtgctg
    56221 ccttcgggtg agaatttcac ggcattgttg atgagattga taaagacctg tgaaagaccg
    56281 cccgcatcac caagcacgct gcaaatatca gaatccacgg ataattcaat ggaaatatcc
    56341 cgcatccgcg caatggattt gaccgcccgc acagattcct gaagaatggg agccaattcc
    56401 atgggaacgg catcgagttt cattcgtccg ctctcgaggc ggccagccat caacaattcg
    56461 ttgatcaaat tcttttggcg gttcagttca ctggtcagcg tgtgccagta ttcgctcatt
    56521 tgctcttctg taccgccttg ctgaagcagt tcggtcatca taatggcggc ggtcaagggg
    56581 gtgcggagtt catgcgaagc acggttgatg aaatcagatt tcatctgatc gagccgcgcc
    56641 tgttcagaaa tatcacggac aatgatgagc gcctcttcgg cactgatggg atgcaggcgc
    56701 gcctcaaaag tctgctggtg atttggcagg ttgaatccgt acacccagtg tgaagataga
    56761 aaggcatcct gatcctcggt ttcgatgatc atattcacaa catcggcagg ccagagttga
    56821 tcgagccgct tccccaccac caggtcacga ggcatataca atggatggct gggatgcgcg
    56881 ttgtaatcca gaatatgccc tgtcaggtcg gtgcgcagaa gcaggtcggg cagggcatcg
    56941 accaaaatac ggttacgcat ttcactaagg cgaatggtct tttcgtagtg caggcgctcc
    57001 aaagctgcgc cgatcaggtt taccgttgtt tgcacggtgt caaactcagc cgtaagccag
    57061 gaaagctcgg tctctttatc aaaaagcgcc agaaatcccc ttgaacccaa agttccctgc
    57121 acgggaatga caagaatgga aactgtaggc acttcctgat tttgcagttt tacattggta
    57181 acggaaaaca cacgttcagg attttccgag atcgaagcaa ggaatggttc aagggttgag
    57241 gtcagatcaa aggtggatgc gtcgtagctt ttccactgga attgaacttt catggaattt
    57301 gccatgatat cgaacaaggc acagcaaaaa acatccacgg catacccgag tgattccaac
    57361 acttcgggaa tcttcgtttc aatatttgat gagcggaaca gcccagccga agcttcacca
    57421 atgacggaaa gcgaaagcat atggcgcttg agattcgctt cagcctgctt gtgttccgaa
    57481 atatcacggg ccagcatttg gatgtaggtg ggcatattgt gttcattatg aaccatagag
    57541 atgctcaact ctacaggcag catcgaacca tcccgcttgc gcagggtgta ttcggatggg
    57601 ttcgtgcgcc tctcaagaag ggtctcgcgc gtttgggaat catcgatcag catgatcgct
    57661 tccacaggca gacccaccag ctggtgttct tcatacgcca gcaatttcaa agcctgaaga
    57721 ttggcagtta cgatcttgaa atcaaggctg ataatgaata cacactcccc tgtttgctca
    57781 aataatgcgc ggtaatcgag gtccgtcccc agtaaatttc tgcctgcatg atgcattttc
    57841 acatccacag gcagtctgct gagagggaca gttttttcat ccatattcaa cgtcttctgg
    57901 ccgcatatct ggcagtgtgg ccacaacgca acatcctttt tcagagtcga cgatctttac
    57961 ttctccgcca agattggaga agacagcccg tgccacggta agcccgacac ccaacccttg
    58021 aaattcgcgc tggtcaccct ggctgatttg ataaaagcgt tcaaatactt tttcgcgcag
    58081 ttcaacaggc acgcccacgc tttcatcctg aattgtgatg gtgccgcccc cattctcacc
    58141 agacctgacc ttcagactca cttgtccatg ctcggggctg aacttgaagg cattatcagc
    58201 gaggtgagca agcgcctgcg aaaattcctt gcgcggcgcc ttgatgggat tcgccaattt
    58261 gatatcgtac acaaagttga ttctcttcag ggggtaacgg tgcagccgtt tctgtaccgg
    58321 ggagagaatg tgatgctcga catcgatcaa ctgtcgggtt gggttgagat cgtgctggtc
    58381 catatccgag agcaaaataa tatccgccac cagcgtgtca agacggtcca cactggcaag
    58441 tgcaatgcgg atgaactcag cctgctcatc aggattttta tattttgtgt tgactgccat
    58501 ttccaatgac atgatgacat tgctcaacgg agtgcgcaat tcatgtctga aattctgcag
    58561 gacctccctg cgaagtttgt ccatctcctg ctgtgccgca tgctgcacct gctcgcggcc
    58621 gcgggcttgt tcagtttgaa cacggcgcaa aactgcgtca atacgcgcca ttaactcctc
    58681 cgttacaaaa ggtttggtta cataatcatc cgcgccgccg cgaattcccg ccacacggtc
    58741 ttcactggct gtacgcgctg taagaaaaat aaaggggatc gatgcaagtt ggggatctgt
    58801 gctcatttgc tttttcatat cgaatccgtt gacgacgggc atcatcacat cagatacgat
    58861 caaagcaggc ggcgatttct tcgccttgac aatggcatcg ctgccattca tggcagtgat
    58921 aacgccatag ccatgacgtt cgatcgtggc tgccagcccc atacgaatgg cgggttcatc
    58981 atccaccact agaataatcg gtttgttttc catggtaatt tcctgattga taaacaccag
    59041 agggtgtttc ctttctacag ttcctgatta tcatcacatc acaccgaaat atcattgtaa
    59101 ggacataaca gtttcggttt ctgctttttc aggctttcaa aggattccac acaatgccag
    59161 cacaaatcca gtcaatccac ccgttataat agggacatgt ttatagacca cgtaacaatc
    59221 catgtaaaat caggcaaggg cggcgatgga atggtgcatt tccgccgcga gaaatttgtc
    59281 ccgttgggtg gccctgacgg gggggatggc ggaaggggtg gaaatgtcat ttttgaagta
    59341 aaagccacgt tgaacacact ttctgcattt cggcccaatg aaaaattcgc cgctgaacca
    59401 ggtaaaaatg ggggcggttc gcagatgaca ggccgtggcg gcaaggacct catcatttac
    59461 gtgcccccag gcactgtgct ctacgatgca gagacaggtg ccctgctggg cgatttgacc
    59521 acccccggtc agcagctttt ggtgtgcaaa ggcggccgtg gcgggcttgg caaccaacac
    59581 tacgccacct cgcgcaatca ggcgccccgt acagcagagc gcggcgagcc ccacgaagag
    59641 aaaatgatcc gccttgagct caaactcatt gcagatattg ggatcatcgg ccttccaaat
    59701 gcgggcaaat ccactttact ggcggcgttg accaatgcaa agccgaagat cggtgattat
    59761 ccgttcacta cgctggaacc aaatctcggt gtggcaaaaa ttgatgacga tacaacagtt
    59821 gtattggcag acatccctgg cttgatcgaa ggcgcagccg aaggtgcagg ccttggtcac
    59881 gactttttgc gtcatattca gcgcacacgg gtattgatcc atatggttga cggtcaatct
    59941 gaagacccgc tggccgatta cacccaaatc aataatgaac ttgcgctttt tgaccccaac
    60001 ctggcaaaaa agccacagat cgtggcgctc aacaaaaccg atcagcccga tgtgcaggaa
    60061 cgtctgaaaa gcattaaggc gagtttcaaa aagaaaaaaa tcgagttatt cacactctct
    60121 gccatggcac gcacaaacac ccgcgatcta ttgatcgctg cgcatcaaag actggccgat
    60181 atgcccgtgg aagaagtaga ggaaaccctg cccgtctaca agccagatgt ggacccgaat
    60241 cacttcgaga tcacccgcga agacgatggc agctggcgca tcaccggcgt ggctattgag
    60301 cgctcggcaa agatgaccta ttttgagcac gatggctcct tgcgccgctt tcagaagttg
    60361 ctgcaacggc tcggagtgga cgacgctctg cgtgaagcag gtgttacaga tggcgacact
    60421 gttttcattg gcaattttga actggaatgg aaggaataac ttccgaggat ctgcatggca
    60481 cagctttctc ccaacaaaca acgagcgctg acgatcggga tgatcgtgct tggggtcatc
    60541 gtcgctgcat tttttggaat gcgtgcattt cgcgctttca acaggttcaa cggtcatcgg
    60601 cctccgccgc aaggcaaggt tgaaaccgat gtcgaactta tccgcggttg gatgaccgtc
    60661 ccattcattt cgcaaatgta tcgtgtccct gatcggatca tcttcgaaga gcttaacatc
    60721 cctgaaaagg gaaacatgga aaaaagtttg aaggacttaa atcaggaata ctaccccaac
    60781 gaggatggct ttgtcatcga aatggtgaaa cagattgttc aggcgaatct ccccaatgaa
    60841 ccacatgatc cgccagagcc accaacaccg ccaaacccgt agggatcttt gatgtctgac
    60901 ttctttctta cacaagttat taattttggt acaccacttg tcgggttgat tctttttctg
    60961 ggtgcgcttg gtttcccagt tggcgcttcg gttgtggtga tcgcagcagg cgccttcggc
    61021 cagcaaggca tattggacat tccctctgca gccatttacg gactgcttgg cgcagtgatc
    61081 ggcgacacac tcagttttgg aatgggatac tacgccaaag atgcggtcca aaggcggttc
    61141 ggaaattcag ccgcatggca gggcgcaact gaaacttttc aaaaaaatgc agggatggcg
    61201 gtttttctta cgcgctggct cattaccgcc attgccatcc ccaccaacct gatcgcaggc
    61261 ggcagcggat ataaatattc ccgcttcatt tcctatgatt taacaggcga aatcgtgtgg
    61321 atcattttgt acggcgggtt gggttactta ttcggcagtc agtgggaatt ggtctatgat
    61381 tttatgagca atttcggcgg gttgatcctg ggcatagtgt tgatggggtt tggcatacgc
    61441 caggcgctgc aatggcaaaa aaacagcagg gataaaatta accaaaaagc tgaggcataa
    61501 gcctcagctt tttacttttt caacctagtg atggtgatgc ccatcttcat ggacatgacc
    61561 gtggtcaagt tcttcttccg ttggttcgcg gagggcaacc actttcacat aaaacgataa
    61621 ctctgcaccc gccagcggat ggttgaaatc taggcggacg gtgtccttgt caaaactggc
    61681 cacgtaggca gcctgatgat tcccatcttc gtctgttacg tgcaattcca tgccttcttc
    61741 cagcttcata tcagcaggaa attcggtgcg cggcacatcc atgaaggcgt catcgtcaaa
    61801 ctcgccgtag ccgtcttcag gttgaaccac cacatccttg ctgtcaccga tcttcatgcc
    61861 catcatttcc ttttcgagcc ctggaatgat gtttccataa ccaacaagga actgcaatgg
    61921 ccctgcttcg tcagacgaat ccaacacttc gccatccact gtcaatttgt agtccattga
    61981 aacgaccaga ccatcctgta ctgtatcttt gctcattcgt ttttcctttt cttgaattat
    62041 cgcgtcgcac aatgtgacaa ttttttacca gatcatgcga tactgtgtat cgcgctcatt
    62101 ttaccagcga attcagcctt catccttgat aattcatcct ttataatctt ttttatgcgc
    62161 tacgacttat gcctgccctg gtactgggaa tatgacgatg ctttcgtcgg gatggtggaa
    62221 cgtacctgta ttgaacaggg cctttccctc tggcagatca agccagataa cctgcttgaa
    62281 tccatcactg ccctgtacaa aggagtgacc actttcaaaa ccctgctcca ccgcggacag
    62341 ggtgaagcca ttttcgaccc aattccccgt tgggcaaagg aattcaacgc gcggcgtatc
    62401 aatcccgccg aagtttcggc atggtctgaa gacaaagcca ccatgcatct cgaattgatc
    62461 agtgcaggtc tgcatacacc tcacaccatt ttgctgccgc cttttctgga gcaacctgtt
    62521 gtgtctgaaa tcaatctggc acccttgggc gggaattttg tcatcaagcc atcgaatggc
    62581 ggcggcggtg aaggggtcat cattggggca aatacgattg aagagattct acatgcccgc
    62641 atggaatacc cagaacaaaa atatttgctt caggcacata tcactccacg cgtcattgaa
    62701 gacacacctg catggttccg tgtcttttat gccaaccagg agacctaccc ctgctggtgg
    62761 aacaccgaga cacacgccta ccgccctgtt tcagcggagg aagagtctca gcaccgcctg
    62821 ggcaggctgc gggatgtaac gaagcggatc gcatccatct gcaagctgga ttggttttca
    62881 acagagatcg ccctcgccga agaattcatc gtcgtggatt atgtcaacga tgaaattgat
    62941 acgcgggttc aatcgcaggc gctggatggc gtaccagacg agatcatgga atgcgttgca
    63001 aaacagctgg taaaaattgc caaggagaat gcatgaaaaa tgtacgagca agagacctgg
    63061 ggattccatt tgaaggactc acaggagaga acaatgcgat cacagacgtt tcaaatgtga
    63121 ccgttgggta tgccaccgtg atcgaaggcc cctcagcgcg gacaggagtg acgatcatcc
    63181 acccgcgtgg agacaaagac cacacacctg tgtttgctgg catccattcg ttcaatggga
    63241 acggcgaaat gacaggcgcg gcatgggtgg aagaaggcgg catgctggaa ggccccatcg
    63301 gaattacgaa cacccacagc gttgggatcg tgcgcgattc catcgtagcc tggcaggtga
    63361 aaaacgatgc gctgtatcaa aagtggtcgt gccctgtagt ggccgaaacc gccgatggct
    63421 ggctgaacga catgaatgga tttcatgtga atgaaaacca tgtgctccag gctttgaact
    63481 ccgcaaaaaa cggctcaatt gaagaaggga acgtgggtgg aggaacgggc atgctgtgct
    63541 acgaattcaa aggcggaacg ggcacatctt cacgcaaact gcccgccaaa ctcggcggct
    63601 ggacggtggg cgtactggtt caagccaact tcggcaggcg ctatcaattg accattgcgg
    63661 gcacgcccat gggacaatat ttcaaagatg atgcgccatt tacgaacggt gaaaatccat
    63721 tcaggcagga tgacggctcg ttgatcgtca ttgtagccac cgatgcgcct gtgctgcctc
    63781 atcaactaaa acgccttgca aaacgtgcca ccatcggcat ggcacggact ggcagtttgg
    63841 gcggcaatgg ctcgggagat attttcctcg ctttttccac cgccaaccca caagccgcac
    63901 atgatggcga aatgggcgtt gccaacattc agtccctctc caacaactat atcgacccga
    63961 tccttgcggc cactgcctat gcaactgaag aagccattat caatgccatg gtggcagcca
    64021 aagatatgaa aggccacaac gacgtggacg ccaaagcatt accgcacgat ggcgtgcggg
    64081 aagtgctaaa acgttacaat agaattggtt gagaaatgtc tgcagtggaa aaagaaagag
    64141 gaaagaaaga aaaaaaaaga agcaggtgga gaaactccat ctgcttcttt tatagatcac
    64201 ttacctaaaa cggcaacttg cattatttac ttcaccaacc ccacgatcac atttacattc
    64261 aatttatcgc cgatctcttc caactcatcg ctccaggcgt ggaaattcaa cttcggcggg
    64321 gccagaacca cacccttcat tgtgaataac ggtgttccgc tcatcggggc tggtgtggag
    64381 cttgtatcca tgttctcaat gttgatgccg cgtgaagcaa gataatgggc aatgtcatgg
    64441 atgatgcctt catggtcagc gcctgtcact tcgatctcat acggcagcca accttcatat
    64501 ttctttgaat cctcggtctg gacaatggat acctgatacc cttcgccgcg caacgactga
    64561 aagtcctggt caatgttgcc tgcttcgtta tcaggcaggg cgatcagcat cagcatggca
    64621 aactccccgc caaggtgtgc catgcggctg gcgcccacgt taccgccgcg cttcactacc
    64681 aaactggtga catggtcaac aagaccgatc cgatccctgc ccgtcagggt caatacaatg
    64741 ttttttggca tggcagactc ccttttacaa ctggtttgtg aaatatatta ccgagtatag
    64801 cataagccca atcaacaaaa aagcccgtga ttttgag
//
LOCUS       DAOQLC010000006        60352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_106_length_60352_cov_20.848898, whole genome shotgun sequence.
ACCESSION   DAOQLC010000006 DAOQLC010000000
VERSION     DAOQLC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 60352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60352
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_60352_cov_20.848898"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1961)
                     /locus_tag="PL000_02215"
     CDS             complement(15..1961)
                     /locus_tag="PL000_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNC87737.1"
                     /translation="MKISPTEYFGNLSLLLDLEAEAEKQQALRERTRLSPTEAEATGN
                     SIINLVIREEGYGLGGRFLLGLGKRNETLPLPWTRLGVGSPVILSEERRDRDASSEET
                     GWRGIVSAVRRDAIQVAVNQFIETEAEKPTFRLDRATDEISRFRQRQAMELARGASGN
                     SRLEVLRDVLIGSVGAVFEKIEPIHVFNKNLNASQIEAVQFALSAEDVAIIHGPPGTG
                     KTTTLVELIRQITHTRSGQTVLAVAASNLAVDNLLERLIAAGENAIRLGHPARVTPEL
                     REHTLDLLVENHPDVKIANKLTRDAYALRDQSFKFTRARPERGAKQAMREEAKDMLNE
                     AWRIEDQVTAMILGKARVVCTTATGIDRERLNGKTFDWCIMDEASQSTEPSAWIPLQF
                     AHRIVLAGDHCQLPPTVLSPEAIRGGFNISLMERLLNNNAPRKMLNVQYRMHQDIMSF
                     SSDVFYEGNLQADAAVQTALLADLPQIAATPLTQTPIHFIDTAGASYDEEPEPDGDSR
                     LNPLEADLVIKKVDELLQCGVSPADIAVISPYSAQVKLLREKILKPSAVDGARSGIEI
                     DSVDGFQGREKEAVIVSLVRSNPEGEVGFLADTRRMNVAMTRARRKLIVIGDSATITA
                     HPFYEKMVKYFESVGAYHSVWEEE"
     gene            2109..2528
                     /locus_tag="PL000_02220"
     CDS             2109..2528
                     /locus_tag="PL000_02220"
                     /inference="COORDINATES: protein motif:HMM:NF026248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HNC87738.1"
                     /translation="MQINLIGPAAALATFLGVWLGHVSVRKIERETVHLWKPTVIAIL
                     LSAGFGTASFLTSSLPLSAICGILSVTLFWDALEIAVRQPKRIKHGHAPANPKNPRHA
                     KILAEYPEATTIDWLDRDPRGTPYSAAELASIKESAK"
     gene            2525..2857
                     /locus_tag="PL000_02225"
     CDS             2525..2857
                     /locus_tag="PL000_02225"
                     /inference="COORDINATES: protein motif:HMM:NF026248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HNC87739.1"
                     /translation="MNWFGFWLCLATLFIIGLGFVWVIRGERYFGHLWWPYVMGTGVL
                     CILGSLFISNNWASALLGAFGASLIWGSTELKEQAVRGEIGWYPFHAKKILPPFVEVI
                     KKWKAPSL"
     gene            2920..4035
                     /locus_tag="PL000_02230"
     CDS             2920..4035
                     /locus_tag="PL000_02230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC87740.1"
                     /translation="MSRTTLIVSCFYIALSACSPAPASPSAALPSPIVTIIQPTETFT
                     ALPITETPTITPSPTALPHLMYPYTIAGLRERTFTGGQIQILQTLMQVETYTRYLISY
                     PSDGLTITGILQVPAGEGPFPVIVMNHGYYNRGEYNSGDGSDRAAAYLVERGYITVSS
                     DYRTWGGSDIGYSFFHTGLVADVMNLIASLPSVPQADVTRVGMWGHSMGGGITTKILT
                     LESPVRAAVLYAPNSADDADLIARWGYGCIGDIAAGELLNTCNAADVIPVTLSDELIA
                     AYVESATSAERMREIAPYYHLENISVPVQIHIGSADGDFIGATPPEWSYKLNQGLLDA
                     GKDVELFVYEGQRHSFTGEAWFTFMQRALDLFDEQLK"
     gene            4055..5149
                     /locus_tag="PL000_02235"
     CDS             4055..5149
                     /locus_tag="PL000_02235"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC87741.1"
                     /translation="MRFWVCLVLFALSACLAAPLSPTPLPAPVETATKIPVTFTPSVT
                     PSPTATRTTQQALYPFTIEGLRKHKFQSGEVVIRETLLETEVFTSYLIEYPSDGLTIT
                     GVLQIPKTGEPPFPVIVMNHGFFSRYIYRSGDGTDRAAEFLNRRGYLTVSSDYRSWAS
                     ADTGESLFYSGLAIDVVNLMNALPSIPEADVTRIGMWGHSMGGGTTLKVLTIDERIKA
                     AVLYSTVSGDFKDIIERWGPGCLGDVLEGEATFGCNSSDILPLTLPDDLIASYFNGVA
                     DPVILKAVSPIYHLKNVTAPVQINYGTEDGKTSAGTPPEWSKKLHEAFQRAGKESQLI
                     GVEGEEHSFKYDQWFYLMERSAQFFDKYVK"
     gene            5158..7641
                     /locus_tag="PL000_02240"
     CDS             5158..7641
                     /locus_tag="PL000_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87742.1"
                     /translation="MKKFLPIIFLLFLLSACASRSSVPATTQPVVAASPNDGEWSGTG
                     NSDEGKSFIVKFTIREEHLAGIVYSFTGADGISCTGIEYGHLDPADDLLIHDDNLSSI
                     LGEDLSFEAAFVDGRTASGQMTIHWHDRQPRCNGDYEVTWSAEKAATPVEQAAQPRNP
                     NAPDPFETFLQILVFGLSNGAVLALNAIGVTLIYGTVRTLNLAHGDVFALTTALVTSI
                     VNIIGIRENWPLPQLIGALFVVFLLAMALGALLSILVEALGFAPFRGSSRLGPMIATL
                     GLSFILFQGALVWRTYQGSWIPGEHRSVPGLPEVPTDGIPSFLPEINLVRYFGLNSNI
                     IIRFSDVFVILSAVVLVAFANWFIQNSKTGRAIRAVSQNPQAAQILGVNIGGTIRKAF
                     AFGGAFAGAAGFIFALYYSRPFGSHGAQSGLFAFMTALLGGIGNPFGALVSGLLIGGL
                     SSLSDYYLTAQWTPALLLLILILLFAWRPTGFTSEGEGGEAAVARDSIILAGPVQGSG
                     GMRWLFIALLILFAFPLAFQGGQVILRLAGIFVLLALGLNLVLGIAGLLDFGFAASFG
                     VSAYLTALFSSKFDFTIALLLGALMGAFLGWLKGGLARRLRSDFFAVATLAFGLLIRQ
                     VIINLDFTGGMGGIGGIPAPHIFGFNFAGQMQKYYLVFFLIVLLAWFSQRVISSRTGR
                     SWMALGDDELAALSVGVDVGRARMRALMLGSAMAGVAGVLYAGGLSFVEPDLMAFHVS
                     SMILTMVILGGAGSVPGALIGAVTIILYDKVFVPQLANWLALIWPVAIGSVPDIRGAS
                     FFNFGIALYLTVLVRSRMASAGSGEARKQ"
     gene            7978..8997
                     /locus_tag="PL000_02245"
     CDS             7978..8997
                     /locus_tag="PL000_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HNC87743.1"
                     /translation="MSKNQLFEYFDELDDQEGTLAQSNHKRVSVRRESKKRDNEARLF
                     IRAQEFSRDFQFTYKAARFEEAWLLDSLAELADLEWISDVLRKVKGGKEASVYLCKGG
                     RAADSPLVAAKIYRPRTLRNLKNDQQYRVGRADLDEKGDVIRDDRTLRATQKRTEYGE
                     EVRHQSWIAYEFLTLETLYAAGADVPKPYAMERNAILMGYVGDVATSAPPLSAVDLDR
                     DEAGQLFERVIRNIDLLLRNDRIHGDLSAYNILYWNGDVTLIDFPQVVAPEANPQAWN
                     IFLRDVTRVCDYFKNQGVWSDPRALAAELWTAYGHKTTKEVEPSLLDAEKPEDRKLWM
                     KQSKL"
     gene            9111..10538
                     /locus_tag="PL000_02250"
     CDS             9111..10538
                     /locus_tag="PL000_02250"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC87744.1"
                     /translation="MNNVRPKLSMLTEEQIQEIHDHTMNVLETVGVRVDSPSALEMLK
                     KKVGASMVNDRLVKIPRELVEWAIKSAPKQVQVYDRRGKPQFTVGGPEDRIRFGIGVT
                     ALYYQEPDTDTPVLFERKHMRDMVRVGNKLPHYDMVSTVGIVRDVPEHLTDLYGSLEH
                     FVNGVKPLVLLVSDEHKFNDVMEMFETLHGDLGEKPFIIPYFNPVSPLVMNEGTVDKM
                     KIAIERGLPVIVSNYSMSGATTPITPAGTIAVLLAELLAGLVIGQLYKEGAPMFLGML
                     PVYFDMKTLMNFYDPQSILVNLACAEMMAHYGLPHCGTSGSGTGWGMDLLAAETYWMN
                     TLTFSLTKGGIAPFVGDTLGSKAISPQTFIYCHEIIDQALRFSSGFQLDEASVGLSEV
                     EKVGPGKSYVSAPTTLKKYKNGYYVNPIFPRWSMEKWIESGQPHAQQKLREYTIEFLK
                     DLPAPEDYEELHAKGEEFIASRLSK"
     gene            10543..10863
                     /locus_tag="PL000_02255"
     CDS             10543..10863
                     /locus_tag="PL000_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87745.1"
                     /translation="MPARVATFVFGIVFAVLFSWLPVYPLLIEGREISESGESLYQEW
                     RFVPLAEYNDSAIFARSAWQKSVRATYVVTTIVNYLVLFGASWGIASLGVRIIKKSAP
                     RLIA"
     gene            complement(10899..12098)
                     /locus_tag="PL000_02260"
     CDS             complement(10899..12098)
                     /locus_tag="PL000_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNC87746.1"
                     /translation="MNNRSLLATRNSEFFLYEELTWDAVAELPRDTPLILPLGSGYNL
                     DLLADQLSNPPRIGLLPPFPFGWRGSGLELPDAIFTQYIVNLMDSLRDDGFSRVYCLA
                     PQGLDPQSSFILQHSASILRQPHPSLYEAQTFLPPDSERGKVILIPIGHTEQHGYHLP
                     LSVDTIIINSIAQGTAAKVPTRSWAMPVMPYGVSTHRSSFAATLNAGGRAFEDFWGAA
                     IDVLVARGFDRFYLMSGHGGNTSFLVNIVKYAGERHRRIFCATAFLHTSGRIGAEALD
                     KYRTSKIGGMGHAGELETSYLLHLRPDLCQMDRVVDETDFVSTPDYYMDWIEGGSLVA
                     NPPWDDDTKTGAYGAGSHATAEKGRLWLEAAIEEKADHVEQIHEQHERREKRRNEGYG
                     LWGKFRK"
     gene            complement(12118..13971)
                     /locus_tag="PL000_02265"
     CDS             complement(12118..13971)
                     /locus_tag="PL000_02265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNC87747.1"
                     /translation="MDNSVKLTEKYERNGWPSVKRPDLNPPAGLNLSLLTSLERIRSH
                     ALSSQGQISCIKDGESLSDVYTMQANGSWLQRISTERPLVPYWDDEIPEWSPDGNWLA
                     FGIKGHVHIVPRDGGLPKKITDFAVAASGPRWMPDSIGLIVTVERNEADQLLLTNYNG
                     DWPRALTTDTIGDHWDARPSPDGKFIVFNLRRFDDLNRLDIVLLEIATGKQTTLYGKP
                     STRAVSPKWSPDGKWISFISQETGREELYLIKPDGEGLHQLTKAGKDIFQYEWSPSGK
                     QMLVVENNGGSFELMLLETESGSRSALRSEKGIHSNPNWAKDESFITFEFEGPTLPPD
                     VYRMELPSKKITQLTFSNPPALQRNKFIVPEVVSYKSYDGLEIPAFLYRPEKSNGAAI
                     LYPHGGPKDQYGFGWDELAQYYVAKGYTYLAPNYRGSTGYGQEFERANYNDWGIGDTQ
                     DCLHGAKYLRTLKTIKPDRIAITGGSYGGYMTICSLSRDPEYLFACGITKYGDSNLVS
                     SWAQCEKRLRVYTEIFLGHPSENLQIYLKGSPITEAKNVQKPVLILHGLLDSIVPPEA
                     SEEWVEALRREGKTFEYKTYGDEPHGFLRRENLLDVYERMERFLDWYLLPS"
     gene            14165..15793
                     /locus_tag="PL000_02270"
     CDS             14165..15793
                     /locus_tag="PL000_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC87748.1"
                     /translation="MKRRFLFILVILTLVLSACGGGNEKAIVRIGWAGSPDNLNPGMG
                     ILTESYTIYNLVYDTMYSLNLDGSFTLSIADSVERSDDGLVYTYKIKDGIKWHDGKPL
                     TAEDVAFSYNLYKDTPEYPYLNGYYTTYFASVESKGNEVILTLTEAIPNIESQLVFLY
                     ILPKHVWEGVNKLEYENFEMIGSGPFKMAEYAQNEFVRLTANKEHFSRPPKVDEVVFQ
                     TFENQDALVQGLKTGQLDMITEMPNTSVETLKAEENVEVVIGAPFSPGVTDIIFNQID
                     PDNCPTDAGGLCTGHPALRDRNVRLAMAHATDKQKLIDLILLGLGTPGLTLIPDGLGV
                     WYNNSIKDYEYDVAAANKILDDAGYKDTDGDKIREMPDGSKPLTFRLNWPSDSINAPR
                     MAELLSEMWGEIGISLEMQAVDPDALTAQCCPAFDFDIMIWGWGSDPDPSALLYVYTS
                     EGIPTGSSETGYSNPKYDELYAQQQTELNFEARKSIVWEMQKLVHDDVVYIIPFYDAE
                     TQAYRSDRFTGWITNQPKVELSDVTSLVLIEPVK"
     gene            15855..16883
                     /locus_tag="PL000_02275"
     CDS             15855..16883
                     /locus_tag="PL000_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87749.1"
                     /translation="MNRYQILLRKTAWALFTILFVIILNFFLFRILPGDPARAGIRDP
                     RLKKESIEILRVRFGLDKPIINCFESLNPIKVGSCEVNPLETQFFIYVRNLLQGEFGF
                     SYHTNRPVGEILAERLWNTVLLIGAGQILSILIGVAFGIIAAWKARTTVDHAAITFSL
                     VAWSLPTFWLGIILLFWGSQNGLPLAGKSTPGMSSMPLLQQWGDLFVHMFLPTLTYTL
                     IYMAEYTLIMRSSLMDVLSEDYILTAKAKGLSNFQILKDHALKNAMLPLVTVIAINLG
                     FTVAGAVQIETVFSWPGLGSAIFEAVNRRDFPVLQGAFLLIAVAVILANFLADLTYTY
                     LDPRVTME"
     gene            16888..18807
                     /locus_tag="PL000_02280"
     CDS             16888..18807
                     /locus_tag="PL000_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HNC87750.1"
                     /translation="METTVQVVETKSRFHGFWRSLRKNRIGFIGLVMLAVIVLLALFA
                     PFIATHDSSSSASAEIGDIYNPPSAEHWLGTDDAGQDVFSNFIFGARISLIVGFFAAF
                     ISLIIGGMLGLVAGFFGGRVDNVIMRFTDIMLVIPDLPLIVVIVALTKPSLLNIIFVI
                     GLLGWTTTARVVRSQTLAVKSRKFVLRARAIGAGRRHIITNHILPLVMPILVVQAVLV
                     VSSAILSESALSFLGLGDPSALSWGQMLNYAFGRGAMSIGAWWALFVPGFGIVWVVLG
                     LTLLGQGLEQVLNPRLDTHHLTPGRPTVQHEAAQPSEQKSEKLLEVRNLSVNYVNEGK
                     PPARAVENVSFTLKPGELIGLVGESGCGKTTLMLALLRLLPSAGQVVNGSVNFHGRNL
                     TELGDDELNQVRWKGISIVFQGAMNALNPVRTVGEQIAEAIIKHLPDFPRDARLQERV
                     VELLDLVGIAADHKDHFPHQYSGGMRQRAMIAMALACNPQVIIADEPTTALDVMIQAQ
                     ILELLDSLRKKLGLAIIFVTHDLGVVAEICDKVLVMYGGVTAEYADVDTIYNEPRHPY
                     TQELIKAFPDLTKPKKKLSSIPGYPPRLDDLPAGCRFAPRCPQAFDRCRTEQPVLYTV
                     KEDHVTSCHLVEASK"
     gene            18804..19796
                     /locus_tag="PL000_02285"
     CDS             18804..19796
                     /locus_tag="PL000_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87751.1"
                     /translation="MSDDQFLKVRGLKKYFDAGRPGLLFGSRTPRYVHAVDGVDFTLR
                     RSEVLALVGESGCGKSTLSLLLMGLEDPTEGNIIFEGRDITHLQDSERKELRRKIQMV
                     FQDPYESLNPTQTIEEIVTEPLIVHNLETEKLERDSRVKKALEDAGLKPAEVYLNRFP
                     HELSGGQRQRVVIAAALVLEPEILLADEPVSMLDVSIRAEIINLLAELRITRQIAVIF
                     ITHDLGSVGFFADRVAVMYLGRIVEIGTMLEVLEKPQHPYTKALLSVIPVPNPRMRRE
                     RIILQGETPNPIDLPTGCRFHPRCPSAMPTCKVSDPPFEVFTKTHQAACLLLVK"
     gene            19820..20599
                     /locus_tag="PL000_02290"
     CDS             19820..20599
                     /locus_tag="PL000_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC87752.1"
                     /translation="MDITSLQNPKIKYIVKLREDKRLRRKDGLMLVEGRDELTLALST
                     GLKPHTLLTAPELATVPMDISSAEITTVTRAVFEKISYRDNPDGWLGLFPIPQTDLDD
                     LKLGANPFVIVAESVEKPGNLGAILRTADAAGVDAVIVCDGRVDVWNPNTVRASRGTV
                     FTVPTVEADSQMTLNWLRLRKMRVLAATPSAEVLHTSVDLSQPIALAVGTEDEGLTDF
                     WMQNADIKVKIPMLGKVNSLNVSIATALITYEAVRQRNGKS"
     gene            20611..21558
                     /locus_tag="PL000_02295"
     CDS             20611..21558
                     /locus_tag="PL000_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNC87753.1"
                     /translation="MKALWLEDNQISLRDVAKPKNPNEALIKIRKAGICSTDLELVKG
                     YYPYTGILGHEFVGEVVEAPDAAWIGQRVVGEINAVCGTCETCLSGRPTHCENRTVLG
                     IVNRDGVLAEYATLPLNNLHRVPDSVSDDMAVFTEPLAAALEIQQQIQLKPDDRVLVI
                     GAGRLGQLIAQTLALTGCDLQVVTRHAHPQSLLAARGIRFTTVDEVQPRKWDVVVEVT
                     GSPDGFNLARKAIRPRGTLVMKSTYKGDMNVNFSSIVVDEIQIVGSRCGPFEPALRLM
                     EKREVDPTVLIMEQYKLNDVVRGFEHAARPGVLKVLIEM"
     gene            complement(21668..21979)
                     /locus_tag="PL000_02300"
     CDS             complement(21668..21979)
                     /locus_tag="PL000_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87754.1"
                     /translation="MSTLREKRTYFGLAALIIGGISVLDLAARFAVAYMDIAPRTFGI
                     LNQITTLFFCILTPLAFVLAVLGWRRKNDSRSQAGIALALVLLPFAGLLLQFILNFFI
                     Q"
     gene            complement(21988..22842)
                     /locus_tag="PL000_02305"
     CDS             complement(21988..22842)
                     /locus_tag="PL000_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNC87755.1"
                     /translation="MLETPSAGRHRQIFWSVLGLLLFLRIPYTLAIIYFLPIENQNGA
                     AVYEVGTYFLTAFLIWWERENLANLHIDTTALLLIIFLRPIQTLILAYWKVETPLVFP
                     GWPSLLIWIIAIGLGLALWQSGFKSTRISFLTLAWLSFGLLIGAVTSIAENFSSFQSM
                     VTHPRAFSSAVLLSTSLNLFYHLGFAPINEEPLFRGFLWAALRQLKWKDGWILVFQAV
                     LFTSAHVYFAGQYPFMFWVFIPAAALLFGWLTLRSRSIAPAMLAHGMINGSVYLWLVS
                     ILSAGSGL"
     gene            complement(22846..23844)
                     /locus_tag="PL000_02310"
     CDS             complement(22846..23844)
                     /locus_tag="PL000_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87756.1"
                     /translation="MKTEIKPFSAEMIPLAGNLLSARHKRNRAKLSLLPERFEDSTVA
                     GKAVEALWQKKFKNGYAAFRDGKMTAYLIGDFTVQPWGRCGYVYLAGYALEEGESLKT
                     LQDLYSLLGEDWVQKGVFSHSLYISAADADVIDALFALGFGKERVDALLDLGIAEIAE
                     PAAPANIDIRIAGTGDNSHLGDLSHVIMESLANAPYWHPTIPEDWEELREGWAELADD
                     QEWTVWLALEKEKALATVGFRPEEEQETAMLVSPRTVYLSIAATLPQARGRGISTALT
                     LCGLKQVQKEGFNICYTNWISPNLPASRFWPRFGFKDAAYRLAKRIDPMIAWTKQR"
     gene            complement(23922..25823)
                     /locus_tag="PL000_02315"
     CDS             complement(23922..25823)
                     /locus_tag="PL000_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87757.1"
                     /translation="MTDELIELEEEEYTSNLTMPVLKRIGGLLKPHWRWVLGFLVTIA
                     LVSSMDAYFTYLNKQIVDQGIKFSDYERVSEIAWMYGSFLILQAAFIFIFIYLAGVLG
                     ERVQYDLRKMLFNHLQDLSLSYYAQNAVGRLIARVTSDTGRVSDLVTWGIVDSVWAVM
                     NITTSLTFMAIINWRLALIVSVIIPIMMFVATKFQKYILVEFRQSRRANSKITGAYNE
                     NIQGVRVVKALGREDENLVEFQQLTTRMYRASYRAAWLSALFLPTVQIIAALALGAIV
                     WYGGVQIQVGLITIGGIQAFVSYLTFMMWPVQDLARVYAEMQHSIASAERIFKLVDTP
                     PEVHNRSDAIEAKTLLGEIEFDHVDFYYEDRKSVLTDFTLKVKPGEMIALVGPTGGGK
                     STIVNLLCRFYEPRNGVIRINGHDYMDYTLESIHNKIGIVLQTPHLFSGTVRENIRYG
                     NLSATDAEVEEAAKIAGAHDFIVTLEKGYEQNVGESGNLLSVGQKQLISLARAVLARP
                     ELFIMDEATSSVDTLTESLIQRGMEALMKGRTSFVIAHRLSTIRRANRILVIENGSIA
                     EQGTHAELLRQRGHYYRLYTQQFRHELEVAYGVTEEIDGNERANVTADSIMVRRERET
                     AGEEAIAAD"
     gene            complement(25873..27711)
                     /locus_tag="PL000_02320"
     CDS             complement(25873..27711)
                     /locus_tag="PL000_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87758.1"
                     /translation="MSTTTTSPYFDFRDALHTNRLVGLWRMMTDFRLSYVAATLALAV
                     SALAKTSVYLLLRFFADDVLKKGTMIGTTMTQTFLYIGLGFFILALFEGGFAFLSGRL
                     AAYTAEGITRRLRDFLFDHIQRLSFSYHSATPTGDLIERVTSDVDALRRFFSEQAIGV
                     GRIILLFVINWVAILYINVKLGLLSVVVIPPILWVSLWFFKKVTKAYEDYQAQEALLS
                     TTLQENLTGVRVVKAFARQDYEKEKFEKDNWGKYLKGKLLLLMHSLFWPLSDIVLGAQ
                     MLFGFVYAAFMAINGEITVGDYVAYVGLVVWLIFPIRNLGRTIVQTSTGMVSYARLMD
                     ITKQAREPLTDGSIQPAGPARGEIEFKNVGFIYSDGTHDVIKDISFHIKPGQSIALLG
                     STGSGKTSLVNLLPRFHDYTSGQILLDGVELKEYPRKYLREQIGIVQQEPFLFSRSIR
                     ENILYGVGRSVTQEEVEAAAKAAAVHDVILTFPDGYNTIVGEKGVTLSGGQKQRVTIA
                     RTILKNPRILILDDSTSSVDTETEASIRNALNNLMENRTTFIIAHRIQSVMKADLILV
                     LDKGQVIQKGNHEELLKQFGGMYRKVYDIQTRIDEELEQEIMKSEL"
     gene            complement(27796..27981)
                     /locus_tag="PL000_02325"
     CDS             complement(27796..27981)
                     /locus_tag="PL000_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87759.1"
                     /translation="MKPANNFHKFLDTSQIKGVNSASQYEKSKERSALLMFNTIFYTF
                     PEAVGILPSRSAPADRR"
     gene            complement(28007..29368)
                     /locus_tag="PL000_02330"
     CDS             complement(28007..29368)
                     /locus_tag="PL000_02330"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC87760.1"
                     /translation="MNQLFVSYSRKDADFARKLTNSLESEKMSVWIDWQDIPPSVDWF
                     KEIEKGIEEADVFIFVVSPDSILSPVCADEVAHAIKNGKRIAPVVAREIDMKDAPKSL
                     SHLNWVFFSRPPDTFEEAFNKLLTAIRTDYDWVQTHNRLQVKALEWDRNKREDSFLLH
                     GMELLDAEAQLKVNGEKQPLPTSLQRTYISASRVAENEFIEAERAEAEQLKLEQEMRL
                     RLRRVTFLVLAVFTLAFFALYAWLSRVTTDLALNSIKNQMLALVETSVSIVDGNEYQS
                     FASAYADPSDTAVYSDEYYQRLTRYVDGIRAGNTNIPGEIVLYTIVKGGNSGEFSVLV
                     STMEDLPFRFSITSNNPSDIHAVGMNETVADPQTIYQNENGGWVCSCAPIYNDNGESV
                     GALCADFDANLVEETKRRVKTILTLGFIAIYPVMIGLTLMATRNVQKRNAQIPAETKP
                     SAS"
     gene            complement(29373..30752)
                     /locus_tag="PL000_02335"
     CDS             complement(29373..30752)
                     /locus_tag="PL000_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNC87761.1"
                     /translation="MFNKIVALYSDREYFNNVFKIALPIIFQQFVYALLNMLGVVFVG
                     QKGDTAVAAVGLAGQIAFLLNLVHFGIISGAAMFTAQFWGRQDIPNLKRVLGLCLMFA
                     ISASAVFFAISQFIPEWFLGIYSKDPEVIAMGAEYVRTFSWTFLFMAVSASYVFVMRS
                     TGSVKLPTYVSVGVLSLNTLLSYVLIFGYFGMPEMGVQGAAVAAVIARALECVILLYV
                     IYAYKFPVAASIKELTDFDLAFTARVIKPMLPVMLNELFWSLGITTYNAIYGRMGTEA
                     LATVNIVSPIEQIAFVVFVGLANATSVLVGNRIGAGKDDEAYLYGGRSIGLGIAGGVL
                     LGLLLQIFKVPALSLFNVSSEVLQNASVMVNIVSIFLWVRVNNMTIVVGILRAGGDTR
                     FSLFLDGIIIWLVGVPFAALGAFYFHLPVYFVYLCVMSEEVTKWILGIFRWRSRKWIH
                     NLASQMEGI"
     gene            30827..31471
                     /locus_tag="PL000_02340"
     CDS             30827..31471
                     /locus_tag="PL000_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87762.1"
                     /translation="MKRLLILLIFVIAACTPASVDITPTPASPLPDASPTETTSPQVD
                     SATNTPVELESNVDILANLPNPDCAALTPADQEGPYYTPDTPERNSLIDEGMQGTKLI
                     LAGFVLTADCLPVPNAWLDFWQANANGEYDNEGYTLRGHQFTDNKGRYYLETILPGEY
                     PQRPIEHIHVKVQLPAGEVFTTQLYFPTQPVNGLTVQLEDKGDHFIAYFNFVMK"
     gene            complement(31533..33227)
                     /locus_tag="PL000_02345"
     CDS             complement(31533..33227)
                     /locus_tag="PL000_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87763.1"
                     /translation="MTDQMIGQQLANFRVERLIGQGGMATVYLGQDVKLQRPVAIKVI
                     DKRYKNDPAYTSRFIKEARMMAKWRHQNLIQIYYADEEKGYSYYVMEYVDGNDLSTVM
                     EMYAEEGELMPIADVLRIGNAVAGALDYAHSQGVIHRDVKPSNVLIANDGRVLVGDFG
                     MAMEVSDGSMGNIFGTPHYISPEQARRSADAVPQSDLYSLGVILYEILTGAVPFNDPS
                     PASIALQHISQPPPSLRSINPEISPAVEAVVLKALDKNPKNRYQTGAQLMSALDEAFQ
                     AANSSPKIPLPPLPVGVPTVRRSGIPMDRITKRNSGKVKGNTIHQPVELPATVRAANQ
                     NRKKINGWLVVLALLLFLGIGGWYLFKNRPASQTDSPTAAFSPAPLNSTVEPAPPTET
                     LISASATPVPPPTVEASPIPTLAQEAAPTAIPTVKYRDGNNLTLFWNETSFHMLNRSG
                     QPRSLSGFSFERLDENGKPNDRFAGHLWENLKFNYIPSKYCVSIKIYKDEDPPYIDPP
                     DCHGGYVSIIQPRMDLDAELFFWTPKEGSSQFRVVWVGEEVARCDISAGTCEVYIP"
     gene            complement(33224..35611)
                     /locus_tag="PL000_02350"
     CDS             complement(33224..35611)
                     /locus_tag="PL000_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87764.1"
                     /translation="MTATNLRTVAKTISVQQLSRLSLPEVEAVVDLVSKVLPAGNVPG
                     MILSGLARLPGRRIPVQKMHQDINALFSGVEQILDSAMYGAIFAGPAAIIWGYQHLLK
                     LAGKDPETAFPEGVWQFYASYALREDTARHTNETHGFDSLLRAHKIQLSPVDRLTAWL
                     MASVTCLHQYDRLLENEWRERVSISTLEESSSERAKRLHRDWQLHLPYRREEDAPDLD
                     YPAYRRFKFDQFLKANLTSLPKQTYEKWEARLNLLTQNSLPAYQRQMSILAYLEPGSY
                     GESRIPFDLTDAQIGIIHRDSYYLFPVCEQGSNKPLNVMTARAQIANLLRSPFSSPSQ
                     IASLTRIKRSALTELRGKLNPMLVNELDNLKFAPILISTDMRSPALPLSELRQTERGI
                     GSHALTIFDTGETMVFDQSHIFFDGAWGTALSEIMTNEALSWARYLSMLPSPTPADTR
                     VYTALTLQLQPADVDLVQQAPHVSPESGAETDRVKLKECLLLRKKFKQRNDQIQLTIN
                     DLLVLYRAIHAATYKPSAHLLEEITKLGATKPDVAAAIRQVVEEGSRTNPAILIPMDA
                     SLKVPRERIYPLNMEVPLHELNLLSLHAQTMKLLAAYEDSKEDRAAIYSSFDQMQRMY
                     LASLAGFGLYLNRAKEIAIQGESASVGAIKLLAHLPSPIQRILNKIPERFELLNNLLK
                     GREVFSNVGAVVPMSTLTRFSTAKDDNEQKQLAWGVLTDAKSVMHISLRDFRPQVATL
                     FSIGRKDLANLITQDYLDAYADGFNKFIGDLSRITQASRETLPTSKTRKRNPENR"
     gene            35721..36548
                     /locus_tag="PL000_02355"
     CDS             35721..36548
                     /locus_tag="PL000_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNC87765.1"
                     /translation="MTISVFQLTKRGPHTIATHARTLDEITRELPQGFYTTFSTLNAG
                     TEVLGLHMHLQRLYVPAKELSFSPAVDEPTLRREIAELARSFLPKEARIRLILAKNDA
                     SMYIVLQQFDALPKKVYEEGVQVVTASLARMAPRVKDTGFISSSNHQRRLVGGDIFEV
                     LLTKEGKILEGMTSNFYAVKKGTLITSQRGILLGVTRRAVLRLARGEGMSVEYHPPQV
                     DENFNEAFLTSSSRGVVPIVSIDGKPVGQGSVGEWVKRLMKAYAEYVERKAERIDGW"
     gene            complement(36574..37608)
                     /locus_tag="PL000_02360"
     CDS             complement(36574..37608)
                     /locus_tag="PL000_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC87766.1"
                     /translation="MSTDLEQKNVSISEAIQQYLRMVERARSRKTMLAYKNALNVFCQ
                     MLNEKSTNPNTTPITQLNEEQFSKFIDYLSPYTTATEQLYLQAVKGFYLFLDSEKLTE
                     VNQSRLTVFMRQRARRPGKHAPPYKIDEIDKILKGAQNVQNLVNLGAEEGEKNAKLRA
                     YRDRAFLFMLAETGLRVHEACNLRRGDIDWNEGRAIITGKGNKQAVIRFTTYALQVVK
                     EYLSQRAELDGASGKQLSSLPLFARHDKGAGKKIKPITTTTGRNIVNECVERFLGQDL
                     VGEITPHTFRHYFVTTTLRATGNLAIAQRLARHENIQVTNRYTQLADEELDKAYYDIF
                     EKDSKWKHDD"
     gene            38174..38839
                     /locus_tag="PL000_02365"
     CDS             38174..38839
                     /locus_tag="PL000_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC87767.1"
                     /translation="MPPAGLKLLYPVKPTFPITQRFGENPQWYPITNGHNGIDWGIPL
                     RTPIYATLPGSVVRVAADNSGYGNHIRISHDNNLLSLYGHMDERNYALVKVGDTVTAG
                     QHIGYSGNTGRSTGPHLHFEVREGSTAFNPEPYLSDNINIPPARTLFKARVLETTQPF
                     LNIRSGPGILYPVIARYSPGDEMEVTDIGGDTVWLQTKKGFVAYRIDKKTLIEILGME
                     DEG"
     gene            complement(38930..41323)
                     /locus_tag="PL000_02370"
     CDS             complement(38930..41323)
                     /locus_tag="PL000_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC87768.1"
                     /translation="MTTTYDIAIIGAGAVGCAMARELSRYQLNIALLEANSDVGMGTS
                     KASTAIWHTGYDAKPGSLEASLLKRSYKLMQEYLPEANIAHELLGGLLIAWNQDQLDT
                     LPKLLNQAHANGEMDVRIISAEEIYQREPHINKGALGGLFVPGEGILCTFSVPIAMAY
                     QAVLNGVELFLNFQVKEIQRQETEDGPIWSIASGSSSVQARYVINAAGLFSDEINAHF
                     GKTSFTVTPRRGQLIVYDKMARPLVNHVLLPVPTSITKGVLISPTVYGNILLGPTAED
                     LPASRKSATETTESGLKFLLEKGKQILPQLLEEEVTATYSGLRAATEHSDYQIEMDSA
                     LRYLCLGGIRSTGISGAMGIAEYGVELLQGAGLALKPKPEFKKVKLPSIGQASMRPHQ
                     NAEMIARNAAYAEIVCHCERVSRGELMDALNAPIPAASVDALRRRTRASQGRCQGFNC
                     HAALTRLAAAEPAPQITFEKETARPVPVVQKDVLIVGGGPAGLSAAVELKKQGIQNIL
                     VVDREPEAGGMPRFCQHTGFGREDLWRMWSGPKYAKYYRDLAEKMDVEVQTSTTILGW
                     AGPAKLSFTSPSGVGEVDAKAVLLATGVRERPRAARLIPGTRPQGVFTTGSLQRFVYQ
                     ERLPVGKRAVIVGAELVSLSALMTLMHAGVKCERMVTEEARHQIEFPYIVMKWALADI
                     LTRTPITPNARISNIFGRKRVEAVELTHASGQTETVECDTVIFTGSWIPEHELARTGG
                     LILNPQTKGPQIDADFRSSVHGVFAAGNLLRGVETADHCAIEGQQAARSIARFLKGK"
     gene            complement(41369..>42141)
                     /locus_tag="PL000_02375"
     CDS             complement(41369..>42141)
                     /locus_tag="PL000_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNC87769.1"
                     /translation="LVDQQAALFGQACFNAGEMKCSFGTGSFLLMNIGDKPKLSNNGL
                     LTTVGWNFNGQTAYAFDGGIFVTGSAVQWLRDNLKFIPDSASSHNAAQRSKDAGVVVI
                     PALQGLAAPHWRTDVQGAMFGLNRSTTSDDIVRATLDGIACRVYEVVTAMSQDLGQRP
                     PHLKVDGGPSGNPYLMQMIADLLNIEVRVSAALEATAIGIANLAGVSALGTSFEALAQ
                     NWKSETSYQPQMKKEDREKKLEQWGRALNAVKAFHGNG"
     assembly_gap    42142..42241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<42242..42684)
                     /locus_tag="PL000_02380"
     CDS             complement(<42242..42684)
                     /locus_tag="PL000_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNC87770.1"
                     /translation="MKYLLGIDQGTTQTTAVIVNEMGEMLEKNSAQLPARFPQAGWVE
                     QEPADIVRTVIEACAPLLDKYDISAVGFDNQGETFVVWDAETGEAVTPAIVWQDTRGQ
                     SICDALAPGVDPNLLRQKTGLLLDSYFSAPKLKWVFENHPEIRKRA"
     gene            complement(42876..>43736)
                     /locus_tag="PL000_02385"
     CDS             complement(42876..>43736)
                     /locus_tag="PL000_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC87771.1"
                     /translation="FGLVAVAAYVLTMPDLNEAAAAGWGSFFYMWGASRMPSWLSIFL
                     AVGMVLVNYVCALAGLTSTSRMMYAFARDGGLPASAALSKVSTVYRTPGTAVWVSAIL
                     AFASTLYAPAYLILAVACAVFLYLSMVMPIAAGLMAEGGPKWKEKGPFNLGGFSKANA
                     VLAVIFGIVLAISGFFPPNEKVFYFTIVFVVALLGFWSKKTAPIGVVVAVVGLALSFI
                     PISEENVMHFLIPDATTSYITIGVAVLAAIITFMTGGEDNRFEGVPEGDKIKERQAKI
                     AEIEKQFGEH"
     assembly_gap    43737..43836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<43837..44734)
                     /locus_tag="PL000_02390"
     CDS             complement(<43837..44734)
                     /locus_tag="PL000_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNC87772.1"
                     /translation="MAGNKSQAHDKQVLHKLGYAQELSRRMSGFSNFAISFSIICILA
                     GGISAFPAAFNALGSGGAFLIWLVGGVLAMSVAVGMGQIASSFPTAGGLYHWSSHLGG
                     KFWGWATAWFNLIGLICVVSSVDVLLYTVFFKDLLLGTALGVDVSGFGTMHQTVFLII
                     ALGTQALLNHYYIDITTKLTDWSGYVILGLTVILIITLFAFSPVALDFSRLTSFQNVT
                     GDAGGAVVPFRTESVAFAFLLGLSYVCYTITGFDASAHTSEETQDAQVNVPKGMWTAV
                     FWSWVFGLVAVAAYVLTMPDLNE"
     gene            44961..46118
                     /locus_tag="PL000_02395"
     CDS             44961..46118
                     /locus_tag="PL000_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87773.1"
                     /translation="MDSLDTLKLLSSQMTFEPDGEPRINSDTPTCFSPKERDHAFTHP
                     AQLPNGQKIVLLKTLLTSACERDCFYCPFRAGRDFRRATFKPDEFASLFSKMNRGGMA
                     EGIFLSSGVAGGGVRTQDKLLDTADILRRKYQFKGYLHLKMMPGSEKDQVYRAMQLAD
                     RVSVNLEAPNTERLAKLAPHKVFIEELLRPLRWVEEIRRSVPAYKFWNGRYPSTVTQF
                     VAGGSDESDLELLNTTNWLMKNVRLKRAYFSAFHPIQDTPLENKPAVNPMREHRLYQA
                     SFLLRDYGFDLEDLPFTKDNNLPIHADPKQAWAQMNLLHNPTEINKADKHELLRIPGI
                     GLKGADAILSARRAYRLRDLASLKKLGIIAERAAPYILLDGRKSAYQLRIF"
     gene            complement(46191..47420)
                     /locus_tag="PL000_02400"
     CDS             complement(46191..47420)
                     /locus_tag="PL000_02400"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNC87774.1"
                     /translation="MNKHRSFFELPNIKKEIIPVASGIPFGFSTVYNPALGIGGVYGA
                     WGDSYDNHSLRDLVAQRYGEPLDDEDIMNLAELGFLSRHHIPPLDKPEDIELEMQIGT
                     RMLQEAARANGWQVGEVDAVLIGAGGPVIPDYVEQICRRAGIPETALKVSVHKACDSS
                     VAGLHLALNPALTRQGHINIAEELLGKKVLVGGIEGLSRFTSHSHDKYALQLFANAAG
                     VIGVIPGETFKFLVGRNHEAFDGEGVLAIHMYYPHPGKNVGDSLIEVTQENPSHIRVA
                     GLMHEPKDGSPISMAGPMGMVKLFVRTGVKVVTEVFYEYAELMKKLGTPEKQIAVGIV
                     HHANYKINQLKAKQLQSAGIEFPMPWLLSDFGNVSAASNMIAFLRQLPNIKPGDNVLF
                     DGFGAGTYYDVLAVTLG"
     gene            47646..48140
                     /locus_tag="PL000_02405"
     CDS             47646..48140
                     /locus_tag="PL000_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNC87775.1"
                     /translation="MMMTTTTTMETLAIKTENKFRTLVTKQSYKKGEMICAMPSENIT
                     DRPNRFTVQIGREVHTHVGKLAALNHSCDPNVILDTKKMVMVARRDIEKGEELSFFYP
                     STEWEMQAPFICICGSANCIHVVAGARFLPLSTLENHYLNPHIREMMIELLNNTQEHL
                     KSLK"
     gene            complement(48212..48634)
                     /locus_tag="PL000_02410"
     CDS             complement(48212..48634)
                     /locus_tag="PL000_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87776.1"
                     /translation="MSRVINPDSVGKERTRLTKSIVLCIREMMKMSQVTPEAKDMASF
                     IALALQSISDGVDASVAAWEKRDYWVKADKFRMEWRWTGQLADKMKSAVLGDDWATIA
                     SLLPQIAMKLNKIEVSDNHRLGKPWENAYALLALHRKL"
     gene            48767..48848
                     /locus_tag="PL000_02415"
     tRNA            48767..48848
                     /locus_tag="PL000_02415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48801..48803,aa:Leu,seq:tag)
     gene            48901..50286
                     /gene="tig"
                     /locus_tag="PL000_02420"
     CDS             48901..50286
                     /gene="tig"
                     /locus_tag="PL000_02420"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC87777.1"
                     /translation="MNIEKQIQEDHSAKLIVDVEQEKMDVYKRRAATKISARSKIPGF
                     RPGKAPYDLVVRTFGEGAVTEQAIDLFIDAEYGNLLKEADVNPGAAGSLESIDSLTPP
                     KLTFRVPLAPEVDLGTYHDIRMEYEWQAPDQAAVDAALEDLRQMYASTETVDRAIELG
                     DYVLVDVNSETAEVTRKGFATFVRKEDRDTEWPYNGFAKELIGLKAGESKTIQHTFPA
                     DWELEELRDKEVTMEVTVKTVRGVTLPDIDDAFAKMTGAGETVDELKEAVKKDVEARS
                     QAEYDDKYFVELIEKIKEVATIKYHEHTLEHEGEHVLSDLSNRLAQQNMDLDTYFKMR
                     GTTREQFIQDEVNPVAKKRLERGLILDEIVRQEKIQIDNEALEAEYNVTLNGLAQQGM
                     DFSKIRGGRKGQKQLSEAIAMESASRVMTRKALDMIKSIATGEYKPIEERQAEASPEA
                     GEASETESKPE"
     gene            50382..50990
                     /locus_tag="PL000_02425"
     CDS             50382..50990
                     /locus_tag="PL000_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNC87778.1"
                     /translation="MIPDFKNVVPMVVENTGRGERAYDIYSLLLKERIVFVGTPIDDQ
                     VANVIVAQLLFLNHEDPEKEIRMYINSPGGQIYAGLAIYDTMQIISNPISTVAVGVTA
                     SFGTVLLTAGTKGRRYALPHATVHMHQPLGGAQGQATDIEIQAKQILRLKALLNGVLS
                     SHTGQPLEVIERDADRDFYLDAQQAVDYGLIDQVIEMPVKSK"
     gene            51131..51976
                     /locus_tag="PL000_02430"
     CDS             51131..51976
                     /locus_tag="PL000_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HNC87779.1"
                     /translation="MTFSIVACDLKEKTWGIAVASKFPAVGAVVPWAAAGAGAVATQS
                     FANTSFGPRGLEMMAAGLSADETLAKLLMDDSDRELRQVGLVDANGKSATYTGSGCFP
                     WAGGVNGKGYAIQGNILANAKVVPAMEKKFLATKGDLPTRLHAALLAGDRAGGDKRGR
                     QSAAIYVVKPNGGYGGYVDRWIDYRVDDHEDPVPRLGELLELHRLYFDKSPENERAAL
                     KGKTLEQMTSILKRAGYLLKKNTAFVDAFNAFIGNENFEERADPNGQWIDKPVLKYLV
                     KKFGK"
     gene            52032..52817
                     /locus_tag="PL000_02435"
     CDS             52032..52817
                     /locus_tag="PL000_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNC87780.1"
                     /translation="MIPSNIKALILDMDGVIWKADAPIGDLPSTFNRIRERGLKFVFA
                     TNNGTKTPEDYQQKLADLGVDIAPSQVVTSAMGIAFMLAQKFPRGTKIFMIGEDGIRV
                     ALEEKGFEILSAENASQAQAFVMGIDRGINFQKVAEATLLVRAGIPFYTTNTDKTFPT
                     PRGEIPGSGAWVSVIQTATNVEPIIAGKPFPFLMELSLEKLGTKKEETLVVGDRLETD
                     IAAGQSVGCPTALVLSGVSTKAQADAWTPKMDIIAASLADLVK"
     gene            complement(52929..53642)
                     /locus_tag="PL000_02440"
     CDS             complement(52929..53642)
                     /locus_tag="PL000_02440"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC87781.1"
                     /translation="MNLKNFDINALKSRPAILWGLVAVLVLAGICTLVYAFRGGKSEQ
                     ASEGDINVIYTNAAATVNAGQTAIALSVTPTATLNTALLVPSPTVAPLATVTTQPLTI
                     LATATKAVPTSGCDMSIYVSDVTVPDGTAMTAGQTFTKTWKVTNTGACTWTATYKLVF
                     VAGDGMNGQSTPIGKEVKPGESVDISVNLTAPAKSGEIKGTWRLSNDKSVAFGDSLTV
                     VIKIGTVPTPTKTPGGYPN"
     gene            53815..54885
                     /gene="rlmN"
                     /locus_tag="PL000_02445"
     CDS             53815..54885
                     /gene="rlmN"
                     /locus_tag="PL000_02445"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC87782.1"
                     /translation="MLIYDFDQKTLLDLLTRWGEPAYRAKQIWQGLYQHLYNSPEQFT
                     NLSKPLRDKLAEGLNFAPLKPKLYLDSSDGQTRKTLFELGDGQVIESVLMRYGDPADN
                     HDTSGRDIRSRRTLCISTQAGCAMGCVFCATGQMGFKRHLSSGEIVAQVMYYAQMLKE
                     ENLSVTNIVFMGMGEPFHNYENMMAAIDRLNDADGFNFGARRMTISTVGLAPQIKRFA
                     DEHRQVNLAISLHAADDDERLAIMPVNKRYKIADVMEACKYYVEKTHRRVTFEWALIN
                     GVNDTPETAKKLAARLRGLLCHVNAIPLNPTTGYEGEATSRERANLFKESLEQAGVPC
                     TIRMRRGIDIAAGCGQLAGSVN"
     gene            55056..>58411
                     /locus_tag="PL000_02450"
     CDS             55056..>58411
                     /locus_tag="PL000_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HNC87783.1"
                     /translation="MEQKQNWLERPIHPALPGLTNEIAIFIGVILLAIITRFYDLGTR
                     VMSHDESLHTYFSWLLYRGQGYEHSPMMHGPFQFHIVALTYFLFGASDFTSRIPAALF
                     SIAIVVMVWYWRRYLGKWGALIAGLLLVISPYMLYYGRYVRNESFVAFSGIVMLYAML
                     RHFETGGKKYLLIMAGALALHFTSKETSFIYTAEALIYLAVYFIVQVTRRPWGEAERD
                     YRSFIISLGVAVLLAGAAVAFGLYSRDAATISATETAMPSDPALQATPPTEPETGAFS
                     PSLILAAAAIAALVAAGVFLIRGYTWDRIRNERSFEMLMVAGTIVLPMLTPFAIKGLE
                     RWLHVSIPTTAPEVQALSGDIKSVLTIGGLLILMFIISTVIGLLWNKEKWWKIALVFW
                     VPFTILYTTVFTNSDGFFTGTIGSLGYWIVQQDVQRGSQPWYYYLLIQIPVYEFLPAI
                     GFLLALVLGFRRKITTSIEEDVVDQNEEFVNLLKQEHEGEENTEEKNFSNTFSLLVWW
                     SIISLIAFTYAGERMPWLTVHITLPMILITGWALGHIIDTANWEKIQSQRVPLTLAAL
                     TVFIVSVMGMVLALNSPTPPFQGKELEQLQATSAFLLPTIMTLLSAVGLTYLFNGWTF
                     HEAKRAFVLLVFAFLAVLTGRAAFRSSYITYDQATEFLVYAHGATGIKEIMQQAEEIS
                     RRTTGGMGVALAYDASAPNTGVSWPFVWYLRDYTNQRSFDQPTRALRDSVVVIVDEKN
                     FDKIEPALGPGYYRVDYIRMWWPMQDYFNLVDERDPTQPFPEDYACSGALSFLKLVKS
                     KDYTTFCEGFTNPQIRAGIIQIWLNRDYTQYAEAKGRTNLTLATWDPAAKMRLYIRQD
                     VAAQIWNYGLAPSAVVPTEDPTEGKYIPLSSDLIFETFEKTPLNAPRSLAFAKDGSMY
                     VADSRNHRIVHISPLGKVINEWGTFADGASVPVGDGTFNEPWGVAVGPDGSVYVTDTW
                     NHRIEKFSADGKFIKAWGFFGQGETPEAFYGPRGLAVDAQGRVYVTDTGNKRVVVFDA
                     DGNFITQFGSAGLDAGQFDEPVGITVDKNGTVYVVDTWNQRIQTFTAVETAGALTFLP
                     AKQWDVYGWFGQSVDNKPFIAVNNAFHVFIT"
     assembly_gap    58412..58511
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            58581..59720
                     /gene="sucC"
                     /locus_tag="PL000_02455"
     CDS             58581..59720
                     /gene="sucC"
                     /locus_tag="PL000_02455"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNC87784.1"
                     /translation="MKLHEYQSKTIFSKYGIPIPKGRVAATAQEARQIAEELGGRVVI
                     KSQVLVGGRGKAGGVKLAKDSAEAEQLATQILGMEIKGLPVRKVLVDEASAIDQEIYF
                     AITDDRAAKKPVMIASAAGGVDIEEVAAKTPEKIIKVNIDPLLGLREYQARDVAVAID
                     LPREHWKDFVKIAMGLWEVYKNSDASLAEINPLVITKDKKLIALDGKMMIDDNALFRQ
                     PNLSELRDTDEDAPSEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDVIKLFGGEPA
                     NFLDVGGGAGAEKVAAAMRIILTDPNVKAVLFNIFGGITRCDEVARGILAAMDEVKPK
                     VPMVVRLVGTNAEEGRRLLENANMITAETLADAAKKSVEAAKKGN"
     gene            59724..>60352
                     /locus_tag="PL000_02460"
     CDS             59724..>60352
                     /locus_tag="PL000_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC87785.1"
                     /translation="MSILVDKNTRLIVQGITGNEGLFHTTQMVAYGTNVVGGVTPGKG
                     GEWVLDGKLPVFDSVKTAKEATDANATIIFVPARFAPDAMFEAADAGIPLVICITEGV
                     AVQDMMRVRNYLDQKKVRLIGPNCPGLLTPGEAKIGIIPGNIAIPGNIGVVSRSGTLT
                     YEVLYAMKNLGMGATTCVGIGGDPVNGTNFLDVLEMFEHDPKTEKVIMIG"
BASE COUNT        14240 a        15424 c        15624 g        14764 t
ORIGIN      
        1 tcatcctttc ctcctcactc ttcttcccac accgaatgat acgcccccac ggactcaaaa
       61 tacttcacca tcttttcata aaacggatgc gctgtgatcg tggcgctatc gccgatgacg
      121 atcagcttac gccttgctcg cgtcatggct acgttcatgc ggcgtgtatc tgcaaggaag
      181 cccacttcgc cttcggggtt cgagcgcaca aggctgacga tgaccgcttc tttttcgcgg
      241 ccttggaatc cgtccacgct gtcgatttca atgccgcttc ttgcaccgtc cacggcggag
      301 ggtttgagta ttttctcgcg cagcaatttc acctgtgctg aatacggcga gatgacagcg
      361 atgtccgcgg gtgagactcc gcactgcagg agttcgtcca cttttttgat gaccaggtcc
      421 gcttcgagcg ggttcaggcg gctgtctccg tcaggttcgg gttcttcgtc gtaactggcg
      481 cctgctgtgt caatgaagtg aatgggagtc tgcgtaaggg gagtcgcggc aatttgaggc
      541 aggtctgcta acagggcggt ttgaaccgct gcatccgctt gcagattccc ttcgtaaaag
      601 acatccgaag aaaaagacat gatgtcttga tgcatgcgat attgcacatt caacattttg
      661 cgcggggcat tgttgttcag caggcgttcc atcaagctga tgttgaaacc gccgcggatg
      721 gcttcgggcg agagcacggt gggaggcagt tggcaatggt cgcccgccag cacaatgcga
      781 tgtgcgaatt gcaggggaat ccacgcgctg ggttcggtgc tttggctggc ttcatccatg
      841 atgcaccaat cgaaggtttt gccgttcagc ctttcgcggt caatgcctgt ggcggtggtg
      901 caaaccaccc gtgccttgcc gaggatcatt gctgtgacct ggtcttcgat gcgccatgct
      961 tcgttgagca tgtctttggc ttcttcgcgc atggcttgct tcgcgccgcg ttctggcctt
     1021 gcacgtgtga acttgaacga ctggtcgcgc agggcgtagg catcgcgggt gagtttgttt
     1081 gcgatcttta catcgggatg attttccacc agcaggtcga gcgtgtgttc gcgcagttcg
     1141 ggcgttacgc gggcgggatg gccgagacga atggcattct cgcccgcggc aatcaatcgc
     1201 tccagcaggt tgtccaccgc gaggttgctt gcagccaccg ccagtacggt ctgtccgctt
     1261 cgcgtgtgtg tgatctggcg gatgagttcc accagcgtgg tggtcttgcc cgtccctggc
     1321 ggcccgtgga tgatggccac atcttccgcg ctcaatgcga attgcaccgc ttcgatctgt
     1381 gaggcgttca ggtttttatt gaatacatgg atgggttcga tcttttcgaa gacggccccc
     1441 accgaaccga tgagcacgtc acgtagcact tccagccgac tgttcccgct tgcgcctcgc
     1501 gccaactcca tagcctgtct ctgtcgaaag cgcgatatct catctgtggc acggtccagc
     1561 cgaaacgtgg gtttctccgc ttccgtttcg atgaactgat tgaccgccac ctgaatggca
     1621 tctctgcgaa ccgcgctgac gattcccctc cagcctgttt cctcgctgga cgcatcgcgg
     1681 tcgcgcctct cttccgacag aatgactggg ctgcccaccc caagccttgt ccacgggagg
     1741 ggcagagtct cgttccgctt gcccaagccc agcagaaagc gcccgcctaa tccatagccc
     1801 tcttcgcgaa tgacaagatt gatgatgctg ttgcccgtcg cctcggcttc ggtaggggaa
     1861 agtctcgtcc gctcgcgcaa ggcctgctgt ttttccgcct ccgcttcaag atcgagcagc
     1921 aacgataggt ttccaaaata ttcagtcggt gaaattttca tggcgggtta ttttaccccc
     1981 tccgcccctg ccctgcgggc gtggcacctc ccccaaatac gacgaacttc ggtcgcattt
     2041 gggggaggtg cccgctaggg cggagggggt cggggctata atgcatcatc ttcacctcag
     2101 gagttaccat gcaaatcaac ctcattggcc ctgccgctgc acttgccacc ttcctcggcg
     2161 tatggctggg gcatgtctct gtccgcaaga tcgaacgtga gacggttcat ctgtggaagc
     2221 cgactgtgat tgcgattttg ctcagcgctg gatttggaac cgcttcgttc ttaacctcaa
     2281 gcctgcctct gtctgcgatc tgcggcatcc tctctgtgac attgttttgg gacgcactcg
     2341 aaatcgctgt tcgccaaccc aaacgcatca agcatggaca tgcgcccgca aacccgaaga
     2401 atccacgtca tgcaaagatc cttgctgaat atcccgaagc cacaaccata gactggctcg
     2461 accgcgatcc gcgcggcact ccatattctg ctgctgaatt ggcatccatc aaggagagtg
     2521 cgaaatgaat tggtttggat tttggctgtg ccttgctact ctcttcatca tcgggttggg
     2581 ttttgtgtgg gtgattcgcg gggaacgtta tttcggtcac ttgtggtggc cgtatgtgat
     2641 ggggaccgga gtgctgtgta ttcttggttc gttgttcatt tcaaacaact gggcatcggc
     2701 tttgcttggt gcattcggtg catccctcat ttggggttcc accgaattaa aggagcaagc
     2761 cgtgcgcggt gagatcggtt ggtatccgtt ccacgcaaag aagatccttc ctccattcgt
     2821 agaggtgatc aagaagtgga aagcgccgag cttgtaaggt aacaccaagt gacagtcacc
     2881 tttaaggtga ctgtcacttt tagccgttac aattccctca tgtcccgaac cactcttatt
     2941 gtttcctgct tctatattgc tctctctgcc tgctctcccg ctcccgcgtc tccctctgcc
     3001 gccctgcctt cgccgatcgt caccatcatt caacccacag agaccttcac cgcccttccc
     3061 atcactgaaa cgcccacgat cactccttcg ccgaccgcgc ttccgcattt gatgtatccc
     3121 tacaccattg cgggtttgcg cgagcgcacc ttcacaggcg ggcagattca gatcctccaa
     3181 actttgatgc aggtggaaac ctacacacgc tacctcatct cctatccttc cgatgggctg
     3241 acgatcacag gcattttgca agtcccagca ggggaggggc cgtttcccgt catcgtcatg
     3301 aatcacggct actacaaccg cggcgaatac aattcaggcg atggctcgga ccgtgctgcc
     3361 gcctacctcg tcgagcgcgg atatatcacc gtctcttccg attaccgcac ttggggtggg
     3421 tctgatatcg gctattcttt ttttcacaca ggcctggtgg ccgatgtgat gaatttgatc
     3481 gcttcgctgc catccgttcc gcaagctgat gtgacccgcg ttggcatgtg gggacatagc
     3541 atgggcggcg gcatcaccac aaaaatactc acacttgagt cgcctgtccg cgcggcggtg
     3601 ttgtacgcgc ccaactccgc cgacgatgcc gacctgatcg ctcgctgggg gtatggctgt
     3661 atcggcgata tcgctgccgg ggaattgctc aatacctgca acgccgccga tgtcattccc
     3721 gtcacgctgt cagatgagct tattgctgca tatgttgaat ccgctaccag cgcggagagg
     3781 atgcgtgaga tcgctccgta ctatcacctt gaaaacattt ccgtccctgt gcagattcat
     3841 atcggctcgg cagatggcga tttcatcggc gccactccgc ctgaatggtc gtataaacta
     3901 aatcaaggcc tgctcgatgc aggcaaagat gtggaactgt ttgtatatga gggccagcga
     3961 cattccttta ccggggaggc ttggttcact ttcatgcagc gcgccctcga tctgttcgat
     4021 gagcaattaa aataagccga tgaaaataaa gtcaatgcgt ttttgggttt gcttggtctt
     4081 gtttgcgctt tcggcctgcc ttgccgcacc gttgagccca acccctctac ccgcgcctgt
     4141 tgaaactgcc acaaagatcc ccgtcacatt tacgccttcc gtaaccccat caccgaccgc
     4201 cacgcgcaca acacagcagg ccttgtatcc attcaccatt gagggattgc gaaagcataa
     4261 attccaaagc ggcgaggtcg tcatccgcga gaccctgctt gagaccgaag tgttcaccag
     4321 ctatctcatt gaatatccca gcgacggcct gaccatcact ggagttttgc aaattccaaa
     4381 gacgggagaa ccgccgttcc cggtcatcgt gatgaatcac ggtttctttt cgcgctacat
     4441 ttaccgctct ggcgatggca cagaccgtgc cgccgagttc ttgaatcggc gcggatacct
     4501 caccgtctct tccgattacc gcagttgggc cagcgcagac acgggcgaaa gtttgtttta
     4561 ctcgggcctt gccattgatg tcgtcaattt gatgaacgca cttccgtcca ttcccgaagc
     4621 cgatgtgacc cgcattggta tgtgggggca cagcatgggc ggcggcacca ctctgaaagt
     4681 gctcactatt gatgaacgca tcaaggccgc tgttttgtat tccacggtca gcggcgattt
     4741 taaagatatc atcgaacgtt gggggccggg ctgtcttgga gatgtcctcg aaggagaagc
     4801 caccttcgga tgcaattctt ccgacatcct tcccctgacc ctgcccgatg acctgatcgc
     4861 atcctacttt aacggggtgg ctgatcctgt tattctgaaa gcggtctctc ccatttacca
     4921 tttgaagaac gtcaccgcac ccgtgcagat caattacggc accgaagatg gcaagacctc
     4981 ggcaggcacg ccgccggagt ggtcgaaaaa actgcatgaa gcctttcaga gggcaggcaa
     5041 agaatctcaa ctgatcggtg ttgaaggtga ggaacattca ttcaagtacg accagtggtt
     5101 ctatttaatg gaacgttcgg cgcagttttt tgataaatat gtgaagtgaa attgccaatg
     5161 aaaaaattcc tcccgatcat ttttctgctt ttcctgcttt cggcttgtgc aagccgttct
     5221 tctgtgcccg cgaccacaca gccagttgtg gccgcttcac ccaatgatgg cgagtggagc
     5281 ggaactggga attccgacga agggaagtct tttatcgtca aattcacgat tcgcgaagag
     5341 catctggctg gcatcgtgta cagttttacc ggtgccgacg gaatcagctg caccggcatc
     5401 gaatacggcc atttggatcc cgccgatgac ctcctcatac atgacgataa cctttcctcg
     5461 atcctcgggg aggacctttc ttttgaagcg gcttttgtgg atgggagaac tgcctcaggg
     5521 cagatgacca ttcactggca cgaccgtcaa ccgcgctgca atggcgatta cgaagtgaca
     5581 tggtccgctg aaaaggctgc gaccccggtg gaacaggccg cccagccgcg caacccgaat
     5641 gcccccgatc ccttcgaaac ctttttgcaa attctggtct tcgggctttc caacggagcc
     5701 gtactggctt tgaatgccat cggcgtcacc ctcatctacg gcacggtccg cactttgaat
     5761 ttggctcacg gcgatgtctt cgctctgacg accgccctcg tcacatccat tgtgaacatc
     5821 atcggcatcc gtgagaattg gcctctgcct caattgatcg gcgcgctttt cgtcgtgttt
     5881 cttttggcaa tggccttggg ggctttattg agcattctgg tggaggcgtt ggggtttgct
     5941 ccgtttcgcg gttcttcgcg tctggggccg atgattgcca cgctcgggtt gtctttcatt
     6001 ctgtttcaag gggcactggt ctggcgcacc tatcaaggtt catggatacc gggcgagcat
     6061 cgcagtgtgc ccggcctgcc cgaagtgccc accgatggca ttccctcgtt tttacctgaa
     6121 atcaatctgg tgcgatactt tgggttgaat tcgaacatca ttattcggtt cagcgatgtg
     6181 ttcgtgattc tttctgcagt ggtgttggtg gcattcgcaa attggtttat tcaaaattcg
     6241 aaaaccgggc gggcgattcg ggcagtttcg caaaatccac aggcggcgca gatccttggg
     6301 gtgaatatcg gcgggacgat ccgcaaggcg tttgcttttg gcggcgcatt tgcaggggcc
     6361 gcggggttta tctttgcctt gtattattcg cgtccatttg ggtcgcacgg tgcgcaaagt
     6421 ggattatttg catttatgac cgcgttgctg ggcggcattg ggaatccgtt tggggcattg
     6481 gtcagcgggt tgttgatcgg cggcctgtct tcgttgagcg attattacct caccgcgcaa
     6541 tggacacctg ccttgttgct gttgattctg atcctgttgt ttgcgtggcg gccaacagga
     6601 ttcacttcag aaggggaggg gggcgaagca gccgtcgcac gtgattcgat cattttggct
     6661 ggacccgttc aagggtcagg tgggatgcgc tggctcttca tcgccctgct gattttgttt
     6721 gcattcccgc tggcgtttca gggtgggcag gtcattctgc gtctggctgg gatttttgtc
     6781 ctgctggcgc ttgggctgaa tcttgtgttg ggaattgcgg gactgctgga ctttggcttt
     6841 gccgccagtt tcggggtgtc ggcctacctg accgctttat tctcttcgaa atttgatttt
     6901 acgatcgcat tgcttttggg ggcgctgatg ggtgcatttc tgggctggct gaaaggcggc
     6961 ctggcacgcc gtttgcgcag cgattttttt gcagttgcca cgcttgcctt tggtctgttg
     7021 attcggcagg tcattatcaa ccttgatttt acaggcggca tgggcggcat tgggggaatc
     7081 cctgcgccgc atatctttgg tttcaatttt gccgggcaga tgcagaaata ctaccttgtt
     7141 ttctttttga tcgttttgct tgcatggttc agccagcgag tgatttcttc gcgcactggc
     7201 cgctcgtgga tggcgcttgg cgatgatgaa ctggccgccc tgtcggtggg tgtggatgtg
     7261 ggacgggcgc gcatgcgggc tttgatgctt ggttcggcga tggctggagt tgccggggtg
     7321 ttatatgctg gcggcttatc gttcgttgaa cctgacttga tggcctttca tgtctcaagc
     7381 atgatcctga cgatggtgat tttgggaggc gcaggcagtg tgcccggagc gttgatcggc
     7441 gcggtgacga tcattttata cgacaaggta tttgttccgc agctggcaaa ctggctggct
     7501 ttgatttggc ctgttgcgat tgggtcggtt cctgatattc gcggcgcgag ttttttcaat
     7561 tttggcattg cgttgtatct taccgttctg gtgcgttctc gaatggcttc ggctggatca
     7621 ggtgaggcac gcaagcagtg atgcctgaat aaaaagcaag ccgtgttttc gcggccatat
     7681 tgcgttcgca cattcttgac attggtttcc cgacaggata aaatcacgtc actatatttc
     7741 actttgaaag gaggcacgaa aaaatgacat ccatcttgtt atctgtcaac attcttgttt
     7801 gcgcgcctcc tgttggtact gtgtagtacg gctttatttc tcgtacattg aaccgcaggt
     7861 gcgccatgcc ctgcggtttt tgattccatg ctgcaaccgc gggccaaaac cctgcgtttt
     7921 tgcttttaac gcggggaaac gtcggatgac ttttacaaaa ataaggtaat taaaaaaatg
     7981 agcaagaacc agctttttga atattttgat gaactcgatg accaggaagg caccctggcg
     8041 caatccaacc ataaacgtgt gtcggtcagg cgtgaatcga agaagcgcga taatgaggcg
     8101 aggttgttca tccgcgccca ggagttttcg cgcgattttc aattcaccta caaggccgct
     8161 cgtttcgagg aagcctggct gttggattcg ctggcggagc ttgccgacct tgagtggatc
     8221 tcggatgtgc ttcgaaaggt gaaaggcggc aaggaggctt cggtttactt gtgtaaaggc
     8281 ggccgggcag ccgattctcc gctggtggct gcaaagatct accgtccacg taccctgcgg
     8341 aatctgaaaa acgaccagca gtatcgggtt ggccgtgctg atctggatga aaaaggtgat
     8401 gtgatccgcg atgaccgtac gttacgcgca acgcagaagc gtactgaata tggcgaagaa
     8461 gtgcggcacc aatcgtggat cgcctacgaa ttcctgaccc ttgaaacctt gtacgcggca
     8521 ggtgcggatg tccccaagcc gtatgcgatg gagcgaaacg ccatcttgat ggggtatgtc
     8581 ggtgatgtgg caacatcggc tccgcctctc agtgcagtgg atctggaccg tgatgaggca
     8641 ggccaacttt ttgagcgtgt gatccgcaac attgacctgt tgcttcggaa tgaccgcatc
     8701 catggagatc tatcggcgta caacatcctg tattggaatg gcgatgttac gttgatcgat
     8761 tttccacaag tggttgcccc ggaggccaat cctcaggcat ggaacatttt cctgcgtgac
     8821 gtcaccaggg tttgcgatta ttttaaaaat cagggagttt ggtcagatcc gcgcgcgctg
     8881 gctgcagagt tgtggacagc atatggtcat aagaccacaa aagaagttga gccatccttg
     8941 ctggatgcgg aaaaacctga agaccgaaag ttgtggatga agcagagcaa gctctgacct
     9001 gaatctgtgc agggaattgg gcaatcgcca tcaaaaggca gccgttgacg agagttgcgg
     9061 ctgccttttt catgccagtg ataaaattgc ggcgaggaga tcataaactc atgaataatg
     9121 tccgcccaaa attgagcatg ttgactgaag aacagattca ggaaatccac gatcacacca
     9181 tgaacgttct tgaaactgta ggggtgcggg tggattctcc ttccgctttg gaaatgttga
     9241 aaaaaaaggt cggcgcatcc atggtgaatg accgattggt gaaaatcccg cgtgaattgg
     9301 ttgagtgggc gatcaaatct gctccgaagc aggtgcaggt gtacgatcgg cgcggaaagc
     9361 cgcagtttac ggtcggcggc cctgaggatc gtatccgctt tgggatcggt gtgaccgcct
     9421 tgtattatca agagccagat acagacaccc cggtattgtt cgaacgcaag cacatgcgtg
     9481 atatggtgcg tgtggggaac aaacttccgc actatgatat ggtgtccaca gtgggaatcg
     9541 tccgcgatgt tccagaacac ctcacagatt tatatggcag cctcgagcat tttgtgaatg
     9601 gggtcaagcc gctggttctt ttggtctctg atgagcataa attcaacgat gtgatggaaa
     9661 tgtttgaaac gcttcacggc gatctgggag agaagccgtt catcatcccg tactttaatc
     9721 ccgtttcacc attggtgatg aatgaaggca ccgtggacaa aatgaagatc gccatcgaac
     9781 gcggattacc cgtcatcgtt tcgaactaca gcatgtctgg tgcgacgacc ccgatcacac
     9841 ccgcagggac gatcgcggtc ttgctggctg aattgcttgc ggggttggtc ataggtcagc
     9901 tttataaaga aggcgcaccc atgttcctcg gaatgctgcc cgtgtatttt gatatgaaga
     9961 ccctgatgaa tttctacgat ccacagagca ttttagtcaa tcttgcctgt gctgaaatga
    10021 tggcgcatta tgggcttccg cattgcggca catcgggcag cggtactggc tggggcatgg
    10081 accttctcgc agccgagact tattggatga atacgctgac cttttctttg acaaagggcg
    10141 gcatcgctcc atttgtcggt gatacgcttg gatcgaaagc gatctccccc cagacattta
    10201 tttactgtca cgagatcatt gaccaggccc tgcgcttctc ttctggcttt caacttgatg
    10261 aagcatcagt gggcttgagt gaagtcgaaa aagtggggcc tgggaagagc tacgtctctg
    10321 cgccgaccac gctcaaaaaa tacaagaacg gctattacgt caacccgatc ttcccacgct
    10381 ggagcatgga aaagtggatc gagtcggggc agccgcatgc acagcagaaa ttgcgcgagt
    10441 acaccatcga atttttgaaa gacctgcccg cacctgaaga ttacgaagag ttgcatgcca
    10501 aaggtgaaga gtttatcgca agccgtttga gtaaataaag tgatgcccgc cagagtcgcc
    10561 acctttgttt ttggaatcgt gtttgccgtg ttattttcat ggctgcctgt ttacccgttg
    10621 ctaattgaag gccgtgagat ttcggaatct ggcgaaagct tgtatcagga atggcgcttt
    10681 gtccctctgg ctgaatataa cgactcagct atctttgccc gctctgcgtg gcagaagtct
    10741 gtccgcgcca catatgttgt gacaacgatc gtcaattatc ttgttttgtt tggggcttct
    10801 tggggaatcg cttccctggg tgtgcgcatc atcaaaaaga gcgcgccaag gcttatcgcc
    10861 tgacgcgctc ttccaatgct tgctggtttc tttccttttt actttctaaa ctttccccac
    10921 aatccatacc cttcatttct tctcttctcg cgccgctcat gctgctcgtg gatttgctcc
    10981 acatgatcgg ctttttcttc gatggcggct tccagccata ggcggccttt ttcagcggtg
    11041 gcgtgactgc ccgcgccgta tgcgcctgtc ttggtgtcat cgtcccacgg cgggttggcg
    11101 accaaagacc cgccttcgat ccaatccatg taatagtcgg gtgtggagac gaagtctgtt
    11161 tcatccacca cgcgatccat ctggcagaga tcggggcgca agtgcagcag gtacgatgtt
    11221 tccaattcgc ctgcgtggcc catgccgccg attttcgatg tgcgatattt atcgagcgcc
    11281 tccgcgccga tacggcctga ggtatgcaaa aatgcagtgg cgcagaaaat gcggcggtga
    11341 cgttctccag cgtatttgac gatgttcacc aaaaacgaag tgttgccgcc gtgtccgctc
    11401 attaagtaga agcggtcaaa accgcgcgcg acgagaacat caatggcagc gccccaaaaa
    11461 tcttcaaagg cacgtccgcc tgcgttcaaa gtggccgcaa aggatgaacg atgtgtgctg
    11521 actccataag gcatgacagg catcgcccaa ctgcgcgtag gcactttggc tgctgtgcct
    11581 tgggcaattg aattgataat aatggtatca acggaaagag ggaggtggta gccgtgctgt
    11641 tctgtgtgac cgatgggaat caggatcact tttccgcgtt cgctatctgg tggaagaaaa
    11701 gtttgtgcct cataaagtga cggatgcggc tggcgaagaa tggatgccga atgttgaaga
    11761 atgaagctcg attgcgggtc aaggccttgt ggcgcgaggc aatagacccg tgaaaatcca
    11821 tcatcgcgca gactatccat gaggttgacg atgtactgcg tgaagatcgc atctggcagt
    11881 tcaagtccgc tgccgcgcca gccgaatggg aacggtggca ggagtccgat gcggggcgga
    11941 ttgcttagtt ggtcagcaag caggtcgagg ttatatccag agccgagtgg gaggatgagc
    12001 ggcgtgtcgc gtggaagttc cgcaaccgca tcccaagtca gttcctcgta caaaaagaac
    12061 tctgaatttc gtgtagccaa taaagaacga ttattcattt taatttttcc tctctattta
    12121 agaaggaagt aaataccaat caaggaatcg ttccatgcgt tcgtaaacat cgagcagatt
    12181 ctcgcggcgt aaaaagccat gcggctcgtc gccataggtt ttgtattcga aggtcttccc
    12241 ttcgcggcgc agggcttcaa cccattcttc gctggcttca ggtggcacaa tgctatcgag
    12301 caggccatgc aaaataagca cgggtttttg cacattcttt gcctcggtga tgggggagcc
    12361 cttgagatag atttgtaaat tttcagatgg atggccgagg aaaatttcgg tataaacccg
    12421 caggcgtttt tcacattgcg cccacgaact gaccagattt gaatcgccgt acttggtgat
    12481 gccgcaggca aaaagatatt cggggtcacg cgaaagcgag cagatggtca tatagccgcc
    12541 ataacttccg cccgtaatgg cgatgcggtc gggtttgatg gttttgagcg tgcgtaaata
    12601 tttggcgcca tgcaaacaat cctgcgtgtc gccgatgccc cagtcgttgt aattggcgcg
    12661 ctcgaattcc tggccgtacc ctgtggagcc gcgataattc ggcgcaagat aggtatagcc
    12721 tttcgcaacg taatactgtg cgagttcatc ccagccaaac ccatattggt cctttggccc
    12781 gccatgcgga tacaaaattg cagcgccgtt cgatttttca gggcggtata aaaatgctgg
    12841 gatttccagc ccgtcgtagc ttttgtatga aaccacttca ggcacgatga atttatttct
    12901 ctgcaacgca ggcggatttg aaaaagtaag ttgtgtgatt ttcttgctcg gaagctccat
    12961 gcgatacaca tcgggcggaa gggtcggccc ttcgaactcg aacgtgatga aggattcgtc
    13021 cttcgcccag tttggattcg agtgaatgcc cttttcgctt cttaatgcgg accttgagcc
    13081 tgattctgtc tcgagcagca ttaactcaaa cgagccgccg ttattctcca ctacgagcat
    13141 ctgttttccc gaaggtgacc actcgtattg gaaaatatct tttccagcct tggtgagttg
    13201 atgcagccct tcgccatctg gcttgatgag atacagttct tcgcgtccag tttcctgcga
    13261 gatgaacgaa atccatttgc catcgggtga ccatttcggc gataccgcac gcgtggacgg
    13321 ttttccgtac aacgtggttt gtttgcccgt ggcaatttca agcaggacga tgtcgaggcg
    13381 gttcaggtcg tcaaagcggc gcaggttgaa gacgatgaat ttgccatcgg gcgaggggcg
    13441 cgcatcccag tgatcgccga tggtgtcggt ggtgagcgcg cgtggccaat ctccgttgta
    13501 attggtaagc agtaattgat ctgcttcgtt tctttcgaca gtgacgatca aaccgatcga
    13561 gtcgggcatc cagcgtggtc cgctggcggc tacggcgaaa tcggtgatct ttttgggaag
    13621 cccgccatcg cgaggaacaa tgtggacgtg gcctttaatt ccaaatgcca gccaattgcc
    13681 atcgggtgac cattcgggaa tctcatcatc ccaatatgga acaagcggcc gttcggtgga
    13741 aattctctgc agccaactgc cgttcgcctg catggtgtag acatcggaaa ggctttcgcc
    13801 gtctttgatg caggagatct gtccctgaga tgaaagcgcg tgagagcgaa tccgctcgag
    13861 ggaagtgaga agcgagaggt ttaatccagc aggtggattc aggtctgggc gtttgaccga
    13921 gggccagccg ttccgttcat atttttcagt gagtttgacc gagttgtcca tggtggtttc
    13981 ctttccattt cgtgccaagt atagcaaaat aggctgaatt acagtacaat ttttacagga
    14041 tacgcttttg gactctctgg aatgtctcta cttttttagc acgttggccc gatgagtacc
    14101 cgcgtcaagg caggtttctg ctttgacgcg ttttgttatc ataatcaacc aaagaggaga
    14161 gaatatgaaa cgaagatttc tgtttattct ggtcattcta acgctggttc tttcagcttg
    14221 cggaggcgga aatgagaagg ccattgtccg cattgggtgg gcaggcagcc ccgataatct
    14281 caacccaggc atgggaattt tgaccgaatc atatacgatc tacaacctgg tctacgatac
    14341 gatgtacagc cttaatctcg atggttcgtt caccctgagc attgccgaca gcgtggagcg
    14401 ttctgacgat ggtttggtat acacctacaa gatcaaggat ggcatcaaat ggcacgacgg
    14461 caagccgttg accgccgaag atgtggcctt cagctacaac ctgtataaag acacccccga
    14521 atatccctac ctgaacggtt attacacgac ctatttcgct tctgtagaat ccaagggaaa
    14581 tgaagtcatt cttacattga ccgaggcaat tcccaatatt gaaagtcaat tggtcttcct
    14641 ttacattttg cccaaacatg tttgggaagg tgtgaataag ctggagtatg aaaactttga
    14701 aatgatcggc tccgggccgt tcaagatggc cgaatatgcc caaaacgaat ttgtgcgtct
    14761 cacggcaaac aaagagcatt tctccaggcc gccgaaggtg gatgaggttg tcttccagac
    14821 ctttgaaaat caagatgcgt tggtacaggg tctcaagacc ggtcagctgg atatgatcac
    14881 agaaatgccg aacacttctg tggaaacttt gaaggctgaa gagaatgtgg aagttgtgat
    14941 cggtgcgccg ttctctccag gcgtgaccga catcattttc aaccagatcg acccggataa
    15001 ctgtcccacc gatgccggcg gtctttgcac gggccacccc gccttgcgtg accgcaacgt
    15061 ccgccttgcg atggcacatg ccaccgacaa acagaaattg atcgatctga tcctgcttgg
    15121 ccttggtacg cccggcctca cgctcatccc tgacgggctt ggtgtttggt acaacaacag
    15181 catcaaagat tatgaatacg atgtggctgc tgcaaacaag atccttgacg atgcaggcta
    15241 caaggatacc gatggcgaca agatccgaga gatgccagat ggctcgaagc cgttgacctt
    15301 ccgcttaaac tggcccagcg acagcatcaa cgctccgcgc atggctgagt tgttgagtga
    15361 aatgtggggc gagatcggta tcagcctcga aatgcaggcc gttgaccccg atgcgttgac
    15421 cgcccagtgc tgccctgcct tcgactttga catcatgatc tggggctggg gctcagaccc
    15481 agacccaagc gcattgcttt atgtttacac ttcggaaggc attccgacgg gttcaagtga
    15541 aactgggtat tccaacccga aatatgacga gctttacgct caacagcaaa ccgaattgaa
    15601 ctttgaggcg cgcaagagca ttgtttggga aatgcagaag ttggttcacg atgacgtggt
    15661 gtatatcatt cccttctatg atgccgaaac tcaagcctat cgctccgacc gcttcactgg
    15721 ctggatcacc aaccagccca aggtggaact ttcagatgta acctctctgg tgctgatcga
    15781 gcctgttaaa taaaccccac agcagggagc ggaagatgcc ttccgctccc tgctcatgtt
    15841 ttcaaggagt taccttgaac cgttatcaga tccttttacg aaaaacagcc tgggcgttat
    15901 tcaccattct gttcgttatt atcctgaatt tctttttatt ccgcatcctg cccggtgacc
    15961 cggcgcgggc gggaatccgt gacccgcgtt tgaagaagga atccattgag attttgcggg
    16021 tccgttttgg gttggacaag cccattatca actgctttga gtcgctgaat ccgatcaaag
    16081 tggggagttg tgaggttaat ccgcttgaaa cgcagttctt tatttatgtt cgcaatctgt
    16141 tgcagggcga attcgggttt agttatcaca caaaccgccc tgtgggcgaa attctagccg
    16201 agcgtttgtg gaatacggtc ctgttgatcg gtgcagggca gattttgtcc atccttatcg
    16261 gggtggcctt tggcattatt gcagcctgga aggcacgcac aacggttgac catgctgcga
    16321 ttaccttcag cctggtggca tggagccttc caacgttctg gctgggcatt atcctgctgt
    16381 tttggggcag tcaaaacggt ttgccgctgg cgggaaaatc cacaccgggt atgagttcca
    16441 tgccgctttt gcagcaatgg ggcgatctct tcgtccacat gttcctgcca accctcacct
    16501 ataccttgat ttacatggcg gaatatacac tcatcatgcg ctcaagtttg atggatgtcc
    16561 tttcagaaga ttatattttg acggcgaagg ccaaaggctt gagcaatttt caaatattga
    16621 aggaccatgc cttgaagaat gcgatgctgc cactggtgac ggtcatcgcc atcaaccttg
    16681 gctttacggt ggcaggtgcc gtccagatcg agacggtctt ctcctggccc gggcttggca
    16741 gcgccatctt tgaagcggtc aaccgtcgtg attttcccgt tctgcaagga gccttcctgt
    16801 tgatcgctgt cgccgtgatc cttgccaatt tccttgcgga tctcacctac acctatcttg
    16861 atccgcgtgt gaccatggag tagcctgatg gaaaccacag tacaggttgt tgaaacaaag
    16921 agtcgtttcc atgggttctg gcgaagcctc cgcaagaatc ggatcggatt catagggttg
    16981 gtgatgctgg ctgtcatcgt tttgctggcg ctttttgccc cttttattgc cacacatgat
    17041 tcatcctctt cagcttcggc tgagatcggc gacatctaca atccgcccag tgcagagcac
    17101 tggctcggca ccgatgatgc aggtcaggat gtcttttcga atttcatttt tggggcgcgt
    17161 atttcgctga tcgtggggtt ctttgccgct tttatctccc tgatcatcgg ggggatgctt
    17221 gggttggtcg ctggcttttt cggcgggcgg gtggacaatg tcatcatgcg ctttaccgat
    17281 atcatgctcg tcatccccga cctgccgctg atcgtggtga tcgtagcgtt gaccaagccc
    17341 tcgctgctta acattatctt tgtcatcggt ttgctggggt ggacgacaac cgcacgcgtg
    17401 gtccgctcac aaacattggc tgtaaaatca cggaaatttg tgctgcgggc tcgtgccatt
    17461 ggcgcaggca gaagacacat catcacgaac cacattctgc cgctggtgat gcccatcctt
    17521 gttgtgcagg ctgtgcttgt agtttcgagc gccatcctta gcgaaagcgc gctttcgttt
    17581 ctggggcttg gtgacccttc cgccctttca tggggtcaaa tgctgaacta cgcttttgga
    17641 cgtggtgcca tgtccattgg tgcgtggtgg gccttgtttg tgcctgggtt cggcatcgtg
    17701 tgggtggtgc ttggtcttac cctgcttggg cagggacttg agcaggtttt aaacccaagg
    17761 ttagacactc atcatttaac accgggcagg cccacggtcc agcatgaagc ggcgcagcct
    17821 tccgaacaaa aatcagaaaa attgctcgaa gttagaaacc tttcggtcaa ttacgtcaac
    17881 gaaggcaagc cgccagcccg ggctgtagag aatgtaagtt ttaccctgaa gccaggtgaa
    17941 ttgatcgggt tggtgggaga aagcggctgc ggaaagacaa cgctgatgct tgccttgttg
    18001 cgcctgcttc catctgcggg gcaggtggtg aatggcagtg ttaatttcca cggcagaaac
    18061 ctgaccgagc ttggcgatga tgaattgaat caagtccgct ggaagggaat ttcaattgtt
    18121 tttcaaggtg cgatgaacgc gttgaatccc gtccgcacgg ttggggaaca gatcgcggaa
    18181 gccattatca agcacttgcc agattttccg cgtgatgcac gtttgcagga acgtgtggtg
    18241 gaactgctgg atctggtggg cattgcggca gatcacaaag accactttcc acaccagtat
    18301 tcgggcggta tgcgccagcg tgcgatgatc gccatggcac tggcctgcaa cccgcaagtg
    18361 atcattgccg acgaacccac caccgcgctg gatgtgatga tccaggctca gatccttgag
    18421 ctgcttgatt cgctgcgcaa gaaacttggg cttgccatta tttttgtaac ccatgacctc
    18481 ggcgttgttg ctgaaatttg cgataaagtg ctggtgatgt atggcggtgt tacggccgaa
    18541 tatgccgatg tggatacgat ctacaatgag ccgcgccacc cctacacaca ggaactgatc
    18601 aaagcctttc cagacctgac caaaccgaag aagaaacttt catccattcc cgggtacccg
    18661 cctcgtttgg atgacctccc tgccggctgc cgttttgcgc cgcgttgtcc gcaggctttt
    18721 gaccgttgcc gcaccgaaca acccgttctg tacaccgtca aggaagacca tgtgacaagc
    18781 tgccatcttg ttgaggcctc aaaatgagcg atgaccaatt tctaaaagtt cgtggattga
    18841 aaaaatattt tgatgcaggc cgtcctggct tgttatttgg cagccgaacc ccgcgttacg
    18901 tccatgcggt ggatggcgtg gattttacgc tcaggcgcag tgaagttctg gctttggtgg
    18961 gcgaaagcgg ctgcgggaaa tccacccttt cgctgctgtt gatgggtctc gaagacccca
    19021 ctgaagggaa catcatcttt gagggccgcg acatcaccca cctgcaagac agcgaacgca
    19081 aggaactgcg ccgcaagatt caaatggtgt ttcaagaccc gtatgaatcc ttgaacccca
    19141 cgcaaaccat cgaagagatc gtcaccgagc cgttgatcgt tcacaacctg gagaccgaaa
    19201 aactcgaacg tgatagccgc gtaaaaaagg cgctggaaga tgcaggcctg aaacctgccg
    19261 aagtgtattt gaaccgtttc ccgcacgagc tttcaggcgg acagcgccag cgtgtggtga
    19321 tcgctgctgc gcttgtgctt gagcctgaaa ttttgctggc agatgaaccc gtttcgatgc
    19381 tggatgtctc catccgtgcc gagatcatca acctgctggc cgaactccgc atcacacgcc
    19441 agatcgccgt cattttcatc acgcacgacc ttggctcggt tggtttcttt gccgaccgtg
    19501 tggccgtcat gtaccttggc cgcattgttg agatcggcac catgcttgag gtacttgaaa
    19561 aaccccaaca cccctacaca aaagccctgc tttccgtcat cccggtgcca aatccgcgga
    19621 tgcgccgtga acgcatcatc ctgcaaggcg aaaccccaaa ccccattgac ctgcccacgg
    19681 gctgtcgctt tcatccacgc tgcccctcgg ccatgcccac ttgcaaggtc agtgacccgc
    19741 cattcgaggt gttcacgaaa acccatcagg ccgcctgcct gcttttggta aagtagggtc
    19801 ggcgctgcat caaaacatca tggacatcac cagtttacaa aacccaaaaa tcaaatacat
    19861 cgtcaaattg cgcgaggata aacgtctgag gcgcaaggat gggttaatgc ttgtggaagg
    19921 ccgcgatgag ttgactctgg ccctcagcac tggactcaag ccgcatacgc ttctcaccgc
    19981 gcccgagctt gccactgttc caatggatat ttcctccgct gagatcacca ccgtcacccg
    20041 cgctgtcttt gagaaaattt cctatcgcga taaccccgac ggctggctgg ggctttttcc
    20101 cattccacaa accgatctgg atgatctgaa attgggcgca aatccgtttg tgatcgtcgc
    20161 tgaatcggtg gaaaagcctg gtaaccttgg cgcaattctg cgcacggccg atgccgcagg
    20221 tgtggacgcg gtcatcgttt gcgacgggcg cgtggatgtg tggaatccaa atacggtccg
    20281 cgccagccga gggacggttt tcaccgtacc caccgtcgag gctgattcgc aaatgacgct
    20341 gaactggttg aggttaagaa agatgcgcgt gcttgcagca acaccttctg ccgaggtgtt
    20401 gcacacttcc gtagacctga gtcaacccat cgcgttggca gtcggcacgg aagatgaagg
    20461 tctcaccgat ttttggatgc aaaatgcgga tatcaaagtt aaaattccca tgctgggaaa
    20521 ggtcaattcg ttaaatgttt cgatcgcgac tgcgctcatc acatatgaag cagtgcggca
    20581 aagaaatggt aaatcgtgag aggtaattcg atgaaggcat tgtggctgga agacaatcaa
    20641 atttcattgc gcgatgtggc aaaaccaaaa aatccaaatg aagcactgat caagatccgc
    20701 aaggcgggaa tttgcagtac cgaccttgaa ttggtgaagg gatattatcc atacacaggc
    20761 attcttggcc atgaatttgt tggcgaggtg gtggaagccc ccgacgccgc atggatcggg
    20821 cagcgggtgg tgggtgagat caacgcagta tgtggaacct gcgaaacctg cctgagcggg
    20881 cgcccaaccc attgtgagaa ccgcaccgtg ttgggcatcg tcaaccgtga tggcgtgctc
    20941 gccgaatatg ccaccctgcc tttgaacaat ctccaccgcg tccctgattc ggtttcggat
    21001 gatatggccg tgtttaccga gccactggcg gcggcattgg aaattcaaca gcagatccag
    21061 cttaagcccg atgaccgtgt gctggtgatc ggcgcaggca ggctgggaca gttgatcgcg
    21121 cagacgctgg cattaacggg ttgtgacttg caagtggtga cccgccatgc tcatcctcaa
    21181 agtttgttgg ctgcacgcgg aattcgattc accacagtcg atgaggtcca gccgagaaaa
    21241 tgggatgtgg tggtggaagt gacaggttca ccagatgggt tcaatcttgc ccgcaaagcc
    21301 atccgtccgc gcgggacatt ggtcatgaaa tccacctaca aaggtgatat gaatgtgaat
    21361 ttctcgtcca ttgtggtgga cgaaattcag atcgttgggt cacgctgtgg tccgtttgag
    21421 ccagccctgc gtttgatgga aaagcgcgaa gtggacccta cagttttgat catggaacaa
    21481 tataaactaa acgatgtcgt tcggggattt gagcacgccg caaggcctgg agtactgaaa
    21541 gtgctgatcg aaatgtgata aggaatttct ttagaaaaac cattttgaac cacggagaca
    21601 ctgaagctcc gtggttattt ttcgggcttg tgcaataagt tttcaggaat cattttgttt
    21661 tggtgggtta ttgaatgaaa aagttaagga tgaattgcag taacagccct gcaaaaggaa
    21721 gcaggaccag agcgagcgcg attcctgctt gagatcgcga gtcgttcttc ctgcgccagc
    21781 cgaggactgc cagcacaaaa gccagcgggg tgagaatgca aaagaacaac gtggtgatct
    21841 gattcaagat gccaaaggtg cgcggggcaa tatccatgta tgcgacagcg aagcgtgccg
    21901 ccaaatcaag gacggatatt ccgccaatga tgagcgcggc caggccaaaa taggttcttt
    21961 tttctcgaag tgtggacatg aatcggatta taaaccagac cctgctgaaa gaatcgatac
    22021 cagccaaaga taaaccgacc cattgatcat tccatgagcc agcatggcgg gggcaatgga
    22081 gcgcgagcgc aaggtcagcc agccgaagag caaggctgcg gcagggatga acacccaaaa
    22141 catgaatgga tattgcccag caaaataaac atgggcggaa gtgaaaagaa cggcttgaaa
    22201 caccaatatc caaccatcct tccatttgag ttggcgcagg gctgcccata aaaatccgcg
    22261 aaagaggggt tcttcattga taggcgcaaa gccaaggtga taaaacaaat tcaggctggt
    22321 ggaaagcagg acggcagatg aaaatgcccg tggatgtgtc accatggact ggaaggatga
    22381 aaaattttca gcgatggatg ttacggcgcc gatcaagagt ccgaagctga gccaggccag
    22441 ggttaagaaa gagatgcgtg tggatttgaa tccactttgc caaagggcaa gccccagacc
    22501 gattgcgata atccaaatca acaggctggg ccatccaggg aaaacgagcg gagtttccac
    22561 cttccagtat gcgaggatga gagtttggat cggtctgaga aaaatgatga gcaacaaggc
    22621 tgttgtgtcg atgtgcaggt tggcgaggtt ttcgcgctcc caccaaatca ggaaggcggt
    22681 cagaaaatag gtgccgactt catagaccgc ggcgccattt tgattttcaa ttggcaggaa
    22741 gtaaatgatg gcaagggtgt aagggattct aagaaaaagg agcaggccga gcacactcca
    22801 aaagatttga cgatgtcggc ctgctgacgg tgtttcaagc atggcttagc gttgttttgt
    22861 ccaggcgatc attgggtcaa tcctttttgc gaggcgatag gcggcatctt tgaaaccgaa
    22921 gcggggccag aaacgggaag caggcaggtt gggactgatc cagttggtgt agcaaatatt
    22981 gaagccctct ttttgaacct gtttcaatcc gcaaagagtg agcgcagtgc tgatgccgcg
    23041 tccgcgagct tgcggaaggg tggcggcaat actcaagtag acagtgcgtg gggataccaa
    23101 catcgcagtc tcttgctctt cttcaggacg gaagcccacg gtggcaagtg ctttttcttt
    23161 ttcaagagcc agccatacgg tccactcctg atcgtctgcc aattcagccc atccttcacg
    23221 cagttcttcc caatcttctg ggatggttgg atgccaatag ggagcatttg ccaatgattc
    23281 catgatgaca tgtgaaagat cgccaaggtg ggaattgtca cctgtgcccg caattcgaat
    23341 atcaatgttg gctggggctg ctggctctgc aatttctgcg atgcctagat ccagcaaggc
    23401 atccacgcgt tccttgccga aaccaagcgc aaacaaggcg tcaattacat ctgcatctgc
    23461 ggctgaaata taaaggctgt gactaaagac acctttctgt acccaatctt ctcccaatag
    23521 agaatatagg tcttgaagtg tcttcaggct ttcaccttcc tccaatgcat acccagccag
    23581 atacacatat ccgcagcggc cccaaggctg aacggtgaaa tcaccgatca agtatgcggt
    23641 catctttcca tcgcggaatg cggcatatcc attcttgaat ttcttctgcc aaagggcttc
    23701 cactgccttg cctgcaactg ttgagtcttc gaatcgttca ggaagcaagg atagtttggc
    23761 gcggtttcgc ttgtgacgcg cagaaagcaa attccctgcc aggggaatca tctctgctga
    23821 aaatggcttg atctcggttt tcatgtttca atttccaatt ctgggttttc atacgaaaag
    23881 caggtcagga tgcaaaccct gacctgcttc actaaattta cttaatctgc ggcaatggct
    23941 tcttcgccag ccgtttcacg ttcgcgcctg accatgatcg aatctgcggt tacgttggca
    24001 cgttcgttgc cgtcaatttc ctcggtgacg ccataggcca cttccaattc atggcggaac
    24061 tgctgtgtgt acaggcggta ataatgcccg cgctggcgga gcaactcagc atgggtgcct
    24121 tgctcggcaa tgctgccgtt ttcgatgacg agaatccgat tcgcgcggcg aatggtggaa
    24181 agacgatggg caatgacgaa agatgtgcgg cccttcatca aggcctccat gcctcgctgg
    24241 atgagcgact cggtcagtgt atccacggag gaggttgcct catccatgat gaacaactca
    24301 ggtctggcaa gcacggcacg tgccagcgag atcaactgct tctgtccaac ggagagcagg
    24361 tttccgcttt cacctacgtt ctgctcgtat cctttttcaa gcgtcacaat gaaatcgtgc
    24421 gcgcctgcga tctttgcagc ttcttccact tcggcatccg tggcagacaa attgccatag
    24481 cggatgttct cgcgaacggt gcctgaaaac aggtgcgggg tttgcagcac gatgccgatc
    24541 ttgttgtgga tcgactcgag cgtgtaatcc atgtagtcgt gtccgttgat gcggatgact
    24601 ccattccgcg gttcgtaaaa tcggcagagc aaattcacga tcgtggactt gccgccgccc
    24661 gtcgggccga ccagtgcgat catttcgcca ggcttcacct tcagcgtgaa atcggtcaat
    24721 acggattttc tgtcttcata gtaaaaatcc acgtgatcga attcgatctc accgagcaga
    24781 gtcttcgctt cgatggcatc gcttctatta tggacctcgg gcggggtatc cactaatttg
    24841 aaaattctct ccgctgaggc aatgctgtgc tgcatttcgg catacacgcg cgccaggtcc
    24901 tgtacaggcc acatcatgaa agtgagatac gaaacgaaag cctgaatacc accgatggta
    24961 atgagcccta cttgaatttg gactccaccg taccaaacaa tggcacccag cgccaacgca
    25021 gcgatgatct gcacagtggg gaggaagagc gccgagagcc aggctgcacg gtaggaagca
    25081 cggtacattc tcgtggtcaa ctgctggaat tccaccagat tttcgtcttc gcgtcccagc
    25141 gccttgacca cacgtacgcc ctgaatattt tcattgtacg cgcctgtgat tttgctgttg
    25201 gcgcggcggc tctggcggaa ctccaccaga atgtatttct gaaacttcgt tgccacgaac
    25261 atcatgatgg ggatgatgac cgaaacgatc aacgccagcc tccagttgat gatggccatg
    25321 aaggtcaacg aggtcgtaat gttcatcact gcccaaacgc tgtccacgat gccccaggtc
    25381 accaggtcag agacacggcc agtgtctgaa gttacacgcg cgataagtcg tcccactgca
    25441 ttttgggcgt agtaggaaag cgataggtcc tgcaggtgat tgaagagcat tttgcgcagg
    25501 tcatattgca cccgttcgcc cagcacacct gcaaggtaaa tgaaaatgaa gatgaacgcc
    25561 gcctgcaaga tcaggaacga gccgtacatc catgcgattt cgctcacgcg ctcgtaatca
    25621 ctgaacttga taccctgatc aacgatctgc ttgttgaggt aggtgaaata agcgtccatg
    25681 ctggagacaa gggcaatggt taccaaaaag cccagcaccc atctccaatg cggcttcagc
    25741 agaccgccga tccgtttcag aacgggcatg gtcaggtttg atgtgtattc ttcttcttcg
    25801 agttcgatca attcgtcagt catggtaatt ctccagaata aaatgaattc agcgggagtt
    25861 gtccgtcaaa attcataatt cagatttcat aatttcctgc tcgagttctt cgtcaattct
    25921 ggtctgaatg tcgtacacct ttcggtacat gccgccaaat tgtttgagca gttcttcgtg
    25981 gttccctttt tggatcacct gacctttatc gagcaccagg atcaagtcgg ctttcatcac
    26041 agactggatg cggtgcgcaa tgatgaaggt cgtgcggttt tccatcaggt tattcaacgc
    26101 attgcggatg gaggcttcgg tctctgtatc tacggaggaa gttgagtcat ccaggatcaa
    26161 aatgcgcgga ttcttcagga tggtgcgggc aatggtcaca cgctgttttt gtcctccgga
    26221 gagggtcacg cccttttcac cgacaatggt gttgtagcca tcgggaaagg tcaggatgac
    26281 atcatgtacg gcggcagctt ttgcggcggc ttcaacttct tcctgtgtca cactgcggcc
    26341 cacgccatac agaatgttct cgcggatcga gcggctgaac aggaacggtt cttgttgaac
    26401 gatgccgatc tgttcacgca ggtacttgcg tggatactct ttcaattcca cgccatcaag
    26461 caggatctgc ccgctggtgt aatcgtggaa gcgcggcagc aaattcacca gtgatgtctt
    26521 gcctgatcca gtggaaccca gcagggcaat ggactgtcca ggtttgatat ggaaggaaat
    26581 atccttgatc acatcatgcg taccgtctga gtaaataaac ccaacgttct tgaactcgat
    26641 ctcgcctcgt gccgggcctg cgggctggat gctgccatcg gtgagaggtt cccgcgcctg
    26701 tttggtgatg tccattaagc gggcgtagga gaccatccct gtcgatgtct gcacgatggt
    26761 gcgcccaaga tttcggattg ggaaaataag ccacaccaca agccctacat acgccacata
    26821 atcaccgact gttatctcgc cgttgatggc catgaaggcg gcgtacacaa acccaaacaa
    26881 catttgggcg ccaagcacga tatccgaaag gggccagaag agtgagtgca tgagcaggag
    26941 caatttaccc ttcaggtatt tgccccagtt gtctttttca aacttctctt tttcgtagtc
    27001 ctgccgtgca aaggctttca ccacacggac tcctgtcagg ttttcctgca gggtggtgga
    27061 gagcagtgct tcctgcgcct gataatcttc gtatgccttg gtcacttttt tgaagaacca
    27121 aagcgaaacc cacaagatcg gcggaatgac caccacagaa agcaagccaa gtttgacgtt
    27181 gatgtacagg atcgcaaccc agttgatgac aaacaacagg atgatccttc caacaccaat
    27241 ggcctgctcg gaaaagaagc ggcgcaatgc atccacatcg gaggtgacgc gttcgatcaa
    27301 gtcaccggtc ggtgtggcgc tgtgatatga aaagctcaag cgctggatgt ggtcgaagag
    27361 gaagtctctc aaacggcggg taatgccctc ggcggtgtag gcagccaaac gtccggaaag
    27421 aaacgcaaat ccgccttcga aaagcgccag gatgaagaat cccaacccga tgtaaaggaa
    27481 ggtttgggtc atcgtcgttc cgatcattgt gccttttttc aagacatcgt ctgcaaagaa
    27541 gcgcaacaaa agatagacgg atgttttcgc cagagctgag actgccaggg ccaaagttgc
    27601 cgcaacgtag gacaaacgga aatccgtcat catgcgccat agaccgacaa ggcggttcgt
    27661 atgcagtgca tcgcggaagt caaaatatgg ggaggttgta gtggtggaca tgtgtggttc
    27721 tccgataaaa agggaaacaa aaaggctgtg gtgagtgcac cacagcccat aaacgaacaa
    27781 gggacagaaa gagacctagc ggcggtctgc aggcgcactc cgagaaggca aaatgccaac
    27841 ggcttcgggg aaggtgtaga aaatggtgtt gaacatcaag agtgcgctcc tttctttgga
    27901 tttttcgtac tgtgaggctg agtttacacc tttgatttga gatgtgtcaa ggaatttgtg
    27961 aaaattgttt gcaggtttca tggggatgat tctaagaaat gcctgcttac gacgcagatg
    28021 gcttggtctc tgctggtatc tgtgcatttc ttttttgaac gttgcgtgtt gccatcaagg
    28081 ttaatccgat catgacgggg taaatggcga taaagccaag ggtcaggatt gttttaaccc
    28141 gcctttttgt ttcctcgaca aggttggcat cgaaatcggc acacaaagcg ccaactgatt
    28201 cgccattgtc attgtaaatg ggcgcgcatg aacacaccca gccgccattc tcattttggt
    28261 aaatggtctg tggatctgcc accgtttcgt tcatgccgac agcgtggata tctgacgggt
    28321 tattgctggt gatcgaaaat cggaagggga ggtcttccat ggtggaaaca agcacagaaa
    28381 actccccgga attccctccc tttacgatcg tgtacagtac gatctctccg ggaatgtttg
    28441 tgttgcctgc cctgatgcca tccacatagc gggtgagcct ttgataatac tcatcgctgt
    28501 acacggctgt gtcagatggg tcggcatatg cgctggcaaa cgattgatat tcatttccgt
    28561 ccacgatgct tacgctcgtt tccaccaatg caagcatctg gttcttaatg gaattcagcg
    28621 ccaggtctgt ggtgacgcgg cttagccagg catagagcgc aaagaaggcg agagtaaaaa
    28681 cagccaacac caggaaggta actcggcgca aacgcagacg catttcctgc tcaagtttaa
    28741 gttgttccgc ctccgcccgt tctgcttcga taaactcatt ttctgccact cgactcgctg
    28801 aaatataggt gcgctgcagg ctggtgggga ggggttgctt ttccccgttg accttcaact
    28861 gcgcctcggc atccagcaac tccatgccat gcagtagaaa actgtcctcg cgcttgttgc
    28921 gatcccattc aagagccttt acctgcaggc ggttatgggt ttgcacccaa tcgtaatcgg
    28981 tgcggatggc ggtcagcagt ttattgaatg cttcctcaaa tgtatctgga ggccgtgaaa
    29041 agaagaccca attcaaatgt gagagcgact tcggtgcatc cttcatgtcg atttcgcgcg
    29101 caaccacagg tgcgatacgc ttgccgtttt tgatcgcatg ggctacttca tctgcgcaca
    29161 caggggaaag gatcgaatcc gggctgacga caaaaataaa aacatccgcc tcttcgatgc
    29221 ctttttcgat ttctttaaac caatccacac tgggaggaat atcctgccaa tcgatccaca
    29281 cgctcatttt ttcagactca agactgtttg taagcttgcg ggcaaaatcg gcatcttttc
    29341 ttgaatagga aacaaaaagc tggttcataa cattaaattc cttccatttg acttgcaaga
    29401 ttgtggatcc acttacgcga ccgccagcgg aaaattccca ggatccattt ggtcacttcc
    29461 tcgctcatta cgcagagata gacaaaatag acaggcaaat gaaaataaaa agcgcccagt
    29521 gccgcaaaag gtacgcccac cagccagatg ataatcccat ctaaaaacag ggagaagcgt
    29581 gtatcgccgc cagcacgcag aatgcccacg acaatggtca tgttgttgac ccgcacccaa
    29641 aggaaaatgg aaacgatatt gaccatcaca ctggcatttt gcagaacctc tgaagataca
    29701 ttgaacaggg aaagcgcggg gactttgaat atctgcaaca gtagccccag cagcactccg
    29761 ccggcaatgc caaggccgat cgaacggccg ccatacagat aggcttcatc gtccttgcct
    29821 gcgccgatgc ggttgcccac caaaaccgag gttgcatttg ccagccctac gaagaccaca
    29881 aacgctattt gttcaatggg cgaaacaata ttgaccgtgg caagcgcctc cgtgcccatc
    29941 cgcccgtaga tggcattgta ggttgtgatt cccagcgacc agaacaactc attcagcatt
    30001 acaggcagca tgggtttgat cacacgggcg gtgaaagcca ggtcaaaatc ggtcagttcc
    30061 ttgatcgagg ccgccacagg gaatttatag gcatagatca catacaaaag gatcacgcac
    30121 tccagagcgc gggcgatcac tgccgccacc gccgcccctt gaacacccat ttctggcatc
    30181 ccaaaatacc caaagatcaa cacatacgaa aggagcgtgt tcaaagaaag aactcccacg
    30241 ctgacgtagg tggggagttt gacactgcct gtggaacgca tcacaaaaac ataactggca
    30301 gaaaccgcca tgaataaaaa tgtccacgaa aaagtgcgga catattccgc ccccatggcg
    30361 atcacctctg ggtctttcga gtaaatcccc aaaaaccatt cagggataaa ttgcgagatc
    30421 gcaaaaaata cagcactggc tgagatcgcg aacatcaaac acaatcccaa cacacgcttg
    30481 aggtttggaa tatcctgcct gccccaaaat tgagcggtga acatggccgc gccgctgata
    30541 attccaaagt ggaccagatt caaaaggaaa gcgatctggc ctgccagccc caccgccgcc
    30601 acagccgtat cgcctttttg ccctacaaag accacgccca acatattcaa caaggcatac
    30661 acaaactgct gaaaaatgat cggcagcgcg atcttgaaaa cattgttgaa gtattcacga
    30721 tcactatata aagctacgat tttattgaac acatcacctc aaggtttgcc attatagcag
    30781 taagagaccc ttcaaaccga ccatctaaaa gcccaaagga gaaccaatga aacgactgtt
    30841 gatcttattg atatttgtga tcgccgcctg cacacctgct tctgtagata taaccccgac
    30901 tcccgcttca ccgttgcctg atgcgtcccc aacagaaaca acctccccgc aagtggattc
    30961 agccaccaac acccccgttg aacttgaatc caatgtggat attcttgcca acctccccaa
    31021 ccccgattgc gccgctctca cccctgcaga ccaggaaggg ccttattaca cacctgatac
    31081 tcctgaacgt aattccttga tcgacgaagg aatgcaaggc accaaactga ttctcgcagg
    31141 cttcgtcctc acagccgatt gtctccctgt gcccaatgcc tggcttgatt tttggcaggc
    31201 caatgcaaat ggagaatacg ataacgaagg ctacaccctg cgcggacatc aattcaccga
    31261 caacaaaggc cgctactacc ttgaaaccat tctgcctggt gaatacccac aacgccccat
    31321 tgaacatatc cacgtgaaag tgcaactccc tgctggagaa gtgttcacca cccagttata
    31381 tttccccacc caacccgtca acggccttac cgtgcagttg gaagataaag gcgatcattt
    31441 tattgcctac ttcaattttg tgatgaaata acagaagcat gattcacaac aaaaaagagc
    31501 ggcaagaaaa ttgccgctct tttttgttgt ctttatggaa tatacacttc gcaagtccct
    31561 gcgctgatgt cacaacgggc cacttcttcg ccaacccaca ccacacggaa ttgagacgag
    31621 ccctcttttg gagtccagaa aaacaactcg gcatccaaat ccatacgcgg ttgaatgatg
    31681 ctgacatagc cgccgtggca atcgggcggg tcaatgtagg gcgggtcttc gtctttgtag
    31741 atcttgatgc tcacacaata ctttgaaggg atatagttga atttgagatt ctcccaaaga
    31801 tgaccagcga atctgtcatt tggttttccg ttttcatcga ggcgttcaaa ggaaaagccc
    31861 gataaactgc gcggctgacc cgatcggtta agcatgtgaa aactggtctc attccagaac
    31921 agggtgaggt tgttaccgtc acggtatttg acggttggaa tggctgtggg tgctgcctcc
    31981 tgtgccaggg ttgggatggg ggaggcctcc accgtcggcg gcgggacggg agtggccgag
    32041 gcggagatca aggtttcagt tggcggggca ggttcaacgg tcgaattaag aggcgcggga
    32101 gaaaatgctg cagtggggga gtctgtttgt gatgctggtc tattcttgaa caggtaccag
    32161 cctccgatcc ccaaaaagag gagaagcgcc agcacaacca accagccgtt gatctttttc
    32221 ctgttctgat ttgccgcacg gacagtggcg ggcaattcca cgggctgatg gatggtgttg
    32281 cctttcacct tgccagagtt gcgcttggtg atgcggtcca tcggaatgcc gctgcggcgg
    32341 acagtgggta cgccaactgg cagcggcggc aacggaattt tcggcgatga atttgccgcc
    32401 tgaaacgctt cgtcaagcgc cgacattaat tgcgcgccag tctggtaacg attctttggg
    32461 tttttatcga gcgccttcaa caccacggct tccacggctg ggctgatctc agggttgatg
    32521 cttcgcagtg aaggcggcgg ttgtgagatg tgctgcaggg caatgctggc tggtgatgga
    32581 tcgttgaacg gcactgcgcc tgtgagaatt tcatacagga tgacgccaag ggaatacagg
    32641 tccgattgag gcaccgcatc tgccgaccga cgggcctgct cgggcgaaat gtaatgcggc
    32701 gtaccgaaga tattccccat ggagccgtcg ctgacttcca tggccatgcc aaaatcgccg
    32761 accaaaacgc ggccatcgtt tgcgatcaac acgttggagg gttttacatc acggtggatc
    32821 actccctgac tgtgggcata atccagtgcg cctgccacag cgttgccgat gcgcagcaca
    32881 tcggcgatgg gcatcaactc accttcctcg gcgtacattt ccatgacggt cgagaggtcg
    32941 tttccgtcca catactccat gacgtaatag gagtagcctt tttcttcgtc ggcgtaatag
    33001 atctggatca ggttttggtg gcgccatttc gccatcatgc gcgcttcctt gatgaagcgg
    33061 ctggtatagg cggggtcgtt cttgtagcgc ttatcgatca ccttgatggc aacaggccgc
    33121 tgaagtttca cgtcctgccc aaggtacacg gtggccatgc cgccctgccc gatcaaacgt
    33181 tccacgcgga agttggctaa ttgctggccg atcatttgat ctgtcatcga ttctctggat
    33241 ttcgctttct tgttttggat gtgggcagcg tttctctgct ggcttgggta attctcgaaa
    33301 ggtcaccgat gaatttgttg aatccgtcgg cataggcatc aagataatcc tgagtgatta
    33361 aattcgcaag gtctttgcgc ccgatcgaga acagggttgc cacttgcggg cggaaatcgc
    33421 gcagggagat gtgcatcaca gatttggcat cggtgaggac gccccaggca agctgctttt
    33481 gttcgttgtc atctttggcg gtgctgaaac gggtgagggt gctcatcggc accaccgcgc
    33541 cgacgtttga aaatacttcg cggcctttca ataaattgtt cagcaattcg aagcgttcgg
    33601 gaatcttgtt caagatccgc tggatggggg aggggagatg ggccaacaac ttaatggcgc
    33661 ccacgctggc gctttcgcct tgaatggcaa tttcctttgc ccgattcaaa tacaggccaa
    33721 acccagccag agaagccaga tacattcttt gcatctggtc aaagcttgag tagatggcgg
    33781 cgcggtcttc ctttgaatct tcgtaggcgg caagcagctt catggtttgg gcgtgcaagc
    33841 taagcagatt caattcgtgg agcggcactt ccatattgag aggataaatc cgttcacgcg
    33901 ggaccttgag actggcatcc atcggaatca ggatcgcagg gttggtgcga ctcccttcct
    33961 caaccacctg gcggatggct gcggcgacat cgggtttggt ggcgccgagt tttgtgatct
    34021 cctccagcaa atgcgccgac ggtttgtacg tggcggcatg gatggcgcgg tacaaaacca
    34081 gcagatcgtt gatggttagt tggatctggt cgttgcgctg tttgaatttt ttccgcagca
    34141 gcagacattc cttgagcttg accctgtcgg tctctgcgcc tgactctggc gaaacgtgcg
    34201 gcgcttgttg cacaaggtct acatcggcgg gttggagctg aagtgtgagc gccgtgtaaa
    34261 cgcgggtatc ggctggagtc ggagagggga gcatgctcaa ataccgcgcc catgaaagcg
    34321 cttcgttcgt catgatctct gaaagggctg tgccccaggc gccatcaaag aaaatatgtg
    34381 actggtcaaa gaccatggtc tcgcccgtat cgaaaatggt cagtgcgtgt gagccgattc
    34441 cgcgttcggt ttggcgcagc tctgagagcg gcaatgcagg agaacgcatg tcggtggaga
    34501 tgaggatggg agcaaacttc aggttatcga gttcgtttac cagcatgggg ttgagtttgc
    34561 cgcgtaactc cgtcagcgcc gaccgtttga ttctcgtcag cgaagcaatt tgagaggggg
    34621 aagagaacgg cgacctgagc aaattcgcga tctgtgcgcg ggcggtcatc acattcaatg
    34681 gcttgttcga gccttgttca caaactggaa agagataata actatcgcga tggataatac
    34741 cgatctgggc gtctgtcagg tcaaagggga tacgcgattc gccgtatgac ccaggctcca
    34801 aataagcaag gatggacatc tggcgttgat aggcaggcag gctgttctgg gtaagtaaat
    34861 tcaaccgtgc ttcccatttt tcgtaggttt gcttcggcag ggaggttagg ttggctttca
    34921 aaaattggtc aaatttgaag cggcgatagg cggggtaatc gaggtcgggg gcgtcttctt
    34981 cgcggcgata gggcaggtga agctgccagt cacgatggag acgttttgcg cgctcactgc
    35041 tcgattcttc cagcgtggag atcgagacgc gctcacgcca ttcgttttcg agcagacggt
    35101 catattgatg caggcaggtg accgaagcca tcagccatgc ggtcagtcgg tcaacagggc
    35161 tcagttgaat tttgtgcgca cgaagcaggg agtcgaaccc gtgggtttcg ttggtgtggc
    35221 gggctgtatc ttcacgcagg gcgtaacttg catagaactg ccagacgcct tctggaaagg
    35281 cggtttcggg atctttgcca gccaatttga gcaggtgctg atatccccaa atgatggcgg
    35341 cagggccagc gaagatcgcc ccgtacattg ccgaatctag aatttgttca acgccactaa
    35401 acagggcgtt gatatcctga tgcattttct gcacagggat gcggcgcccg ggcaggcggg
    35461 ccaatccgct caggatcatg ccaggcacat tgccggcagg aagaaccttg gaaacgagat
    35521 ctacaaccgc ttccacttct ggcagggaga ggcgtgaaag ctgctggacg gagatcgttt
    35581 ttgcaaccgt ccgcaggttg gttgcggtca tttgagatgc ttgctgtaga tctgattttt
    35641 ttccactcat ggctggtgcg taattataat tgcacttgtc catgctcgta ttttttcttt
    35701 caaaagtgta aaatcaactt atgacaatct ctgtttttca acttaccaaa aggggaccac
    35761 acaccattgc cacccatgcg cggactctcg atgagatcac ccgcgaattg ccgcaggggt
    35821 tctacactac attttccacc ctcaacgcag ggacagaggt ccttggtttg cacatgcatt
    35881 tgcagaggtt gtacgtccct gcaaaggagc tgagcttttc gcctgccgtg gatgagccaa
    35941 ccttgcgtcg ggaaatcgcc gaactggccc gatccttttt gccaaaagag gcgcggatcc
    36001 gtttgattct ggcgaagaac gatgcttcca tgtatatcgt ccttcaacag tttgatgcgc
    36061 ttccgaagaa agtttatgaa gaaggagtgc aggtggtgac agcgtcgctg gcacgcatgg
    36121 ccccgcgagt gaaggacacg ggttttattt catcgagcaa tcaccagcgc aggctggtgg
    36181 gcggagacat atttgaggtg ttgctcacaa aggaaggaaa aattctggag gggatgactt
    36241 cgaacttcta tgccgtcaaa aaggggacgt tgatcacatc ccagcgcgga atcctgcttg
    36301 gggtcacgcg ccgcgccgtg ctgcgcctcg caagagggga ggggatgtcc gtcgaatatc
    36361 atcccccgca ggtggatgaa aacttcaacg aagcttttct cacgtccagt tcgcgcggcg
    36421 tggtgccgat cgtttccatt gacggaaagc cggtagggca ggggagtgtg ggggaatggg
    36481 tgaagcggtt gatgaaggcg tacgcggaat atgtggagcg aaaggctgag aggattgatg
    36541 gctggtaagg ttggaactta cgcagggggc aattcaatca tcatgcttcc atttcgagtc
    36601 tttttcaaaa atatcgtaat atgccttatc caactcctcg tcagccagtt gggtatatct
    36661 gtttgtgact tgaatattct catgccgtgc aaggcgttgg gcaatggcaa gattgcctgt
    36721 agctcttagg gttgtggtca caaaataatg acggaacgtg tgtggcgtga tttcgccaac
    36781 caggtcttga cctaaaaaac gctccacaca ttcattgacg atgtttctgc cagtggttgt
    36841 agtgatcggt ttgatctttt ttcctgcacc tttgtcgtgc cgggcaaaga gggggaggga
    36901 acttagttgt ttaccagagg ccccgtccaa ttcagcgcgt tgcgacaaat attctttcac
    36961 gacttgcaaa gcatatgtgg tgaatcgaat cacagcttgt ttgttgcctt tccctgtaat
    37021 gatggctctg ccttcgttcc aatcgatatc gccgcgcctt agattgcagg cttcatgaac
    37081 acggagccca gtttcagcca gcatgaacaa aaaagcgcgg tcgcgatatg cgcgtagctt
    37141 agcatttttt tcgccttctt ctgcacctaa attgaccaga ttttgaacgt tttgtgctcc
    37201 ctttagaatt ttgtcaatct cgtcaatctt atatggcgga gcatgtttgc ccggacgcct
    37261 tgcacgctgt cgcataaaca ctgtgagccg cgattgattg acctcggtca atttttcaga
    37321 atccagaaaa aggtagaagc ccttgacggc ctgcaaatac aattgctctg tggcagtagt
    37381 gtagggactg agatagtcta taaattttga gaactgttct tcgttgagtt gtgtaatggg
    37441 ggtggtgttt ggatttgtag atttttcatt cagcatttga cagaaaacat tgagcgcgtt
    37501 cttatacgca agcattgtct tcctgctgcg agcgcgttcc accatgcgca aatattgctg
    37561 aattgcttct gaaatgctga cgtttttttg ttcaaggtct gttgacatgg cctcattata
    37621 tgggttttcc tcaggttgtc aatgctttcg ctaataatac ttatcgaaag cataagaggt
    37681 gaaaacgaaa cgatggaaat aatttcaccc gccctgctcc cccacgcgga tttttgcgca
    37741 ggccagatca cagcccaaat ttcaccgacg cagggaatcg ggcgacgagc ttttattcat
    37801 ccttttgcct tcatcgttca tccttgtgtc cctcccctgc cccacagatg cggacgtgga
    37861 attcttgcaa aatgacgcat cccccgcatt tctgccgggg gatgcaatat gggttacttt
    37921 caaatttggt cgggaaaaga gttacttctt gggcggagcg gtcttttcct tcttcttctt
    37981 tcgcttttcc ttgttcttgc cttgttcctt gtttgccatg gatggctcct ttcgtgaaat
    38041 gattggatcc tgtatgaaaa cgccgcaaac gtgaccgtca gaacaaatta tacacccgcg
    38101 agattgtatc gtccgtgtca atttgctaca atgaatgcaa ccaattggat gaaacacaaa
    38161 aaaggagaac accatgcccc ctgccggact caaactgctt tacccggtga aacccacctt
    38221 ccccatcacc cagcgcttcg gtgaaaaccc gcagtggtac ccgatcacga acggtcacaa
    38281 cggcattgat tggggcattc ccctgcgcac ccccatctat gccaccctgc cgggcagcgt
    38341 ggtccgcgtg gcggctgata acagcggcta cgggaatcac atccgcatca gccatgataa
    38401 caacctgctt tcgctctacg gccacatgga tgaaaggaat tacgccctcg tcaaggttgg
    38461 cgatacggtc acagcgggcc agcacattgg ctactctggg aatacgggcc gcagcacggg
    38521 gccgcacctg cattttgaag tccgcgaagg gagcacggcc ttcaaccccg agccgtacct
    38581 cagcgataac atcaatattc cgcccgccag gacgcttttc aaagcccgtg tgttggagac
    38641 cacgcagccc ttcctcaaca ttcggtcggg cccgggcatc ctgtacccgg tcatcgccag
    38701 gtacagcccc ggcgacgaaa tggaagtgac cgacatcggc ggcgatacgg tctggctgca
    38761 aaccaaaaag ggatttgtag cctaccgcat tgacaaaaag accctcatcg agatcctcgg
    38821 catggaagat gaaggctgaa gcataaaatt tgtccgccac acaaaagccc accccgaaac
    38881 attcatttcg gggtgggctt ttcattaggc tccggcctgc cgtcagcggt catttccctt
    38941 tcaaaaaccg cgcgatggat cgtgccgcct gctggccttc aatggcgcaa tggtcggcgg
    39001 tttccacgcc gcgcagtaaa ttccctgccg cgaagactcc atgcaccgac gagcggaaat
    39061 ccgcatcgat ctgtggaccc ttcgtctgtg ggttgagaat caggccgccg gtgcgagcca
    39121 gctcatgttc ggggatccag cttccggtga agatgaccgt atcacattcc acggtttcag
    39181 tttgtccgct tgcgtgggtc aactccacgg cttccacccg cttgcggccg aagatgttcg
    39241 atatccgcgc gttgggtgtg atcggggtgc gggtgaggat atcggcaagc gcccacttca
    39301 tcacgatgta tggaaattcg atctggtgcc gcgcttcttc cgtgaccatc ctttcgcatt
    39361 tcaccccggc atgcatcaag gtcatcaacg ccgaaagact caccaattcc gcaccgacga
    39421 tgacggcccg cttccccact ggcagccgct cctgatatac aaagcgctgt aacgaccccg
    39481 tggtaaacac accctgcgga cgtgtgcccg gtatcaaccg cgccgcccgc ggacgttcgc
    39541 gcacgccggt ggccagcagc actgcttttg catccacctc ccccacccca ctgggcgaag
    39601 tgaaagacaa ctttgcaggg ccagcccatc ccaaaatcgt cgtggaggtt tgtacttcca
    39661 catccatttt ttcggcgagg tcgcggtaat actttgcgta tttcggtccg ctccacatac
    39721 gccacaggtc ttcacggcca aaccccgtgt gctgacaaaa acgcggcatg ccgccagcct
    39781 caggttcgcg atccacaacg aggatgttct ggatgccttg cttcttcaac tcaaccgccg
    39841 ccgaaagtcc cgcaggcccg ccgccgacga tcaaaacatc tttttgcaca acgggcacag
    39901 ggcgcgccgt ttctttttca aaagtgattt gaggcgcagg ttctgcagcc gccagccttg
    39961 tcagcgcggc atggcaattg aatccctggc accttccttg cgaggcacgg gtgcgccttc
    40021 gcagggcatc cacactggca gcgggaatgg gcgcattcaa ggcgtccatc aattcgccgc
    40081 gtgaaacccg ttcgcagtga caaacgatct ctgcatacgc cgcattccgc gcgatcatct
    40141 ctgcgttttg atgcgggcgc atcgaagcct gcccaatgct cgggagtttt acttttttga
    40201 attcgggctt cggtttcaat gcaagcccgg caccctgcaa taactccacg ccgtattcgg
    40261 caatgcccat cgcaccggaa atacccgttg agcggattcc acccaaacac agataacgaa
    40321 gcgccgaatc catctcgatc tgataatcgc tgtgttccgt cgccgcgcgc aagcctgagt
    40381 aggtcgccgt tacttcttcc tccaacaatt gcggcaggat ctgtttgccc ttttcaagca
    40441 aaaactttaa tccgctttca gtcgtttcgg ttgcagattt acgcgaagcg ggcaggtctt
    40501 ctgcggtggg ccccagcaaa atatttccgt acaccgtcgg gctgatcaac acaccttttg
    40561 tgatggatgt cggcaccggc agcagtacgt gattaacaag cggacgcgcc atcttgtcat
    40621 agacgatcaa ctgtccgcgt cgcggcgtga ccgtgaaact ggtctttcca aaatgggcat
    40681 tgatctcgtc tgaaaacaat cccgccgcat tgatgacata gcgggcctgc accgacgacg
    40741 agccgctggc tattgaccat atcgggccgt cttcggtttc ctgccgttga atttccttta
    40801 cttgaaaatt caggaacaac tccacgccgt tcaacacagc ctgataagcc atcgcaatgg
    40861 gaacggaaaa cgtgcaaagg attccctccc cgggcacaaa gagtccgccc aatgcgcctt
    40921 tgttgatatg tggttcacgc tggtagatct cttccgcaga aataatgcgg acgtccattt
    40981 caccgttggc atgggcctga ttcaacaatt tgggcagtgt gtccagttga tcttgattcc
    41041 atgcgatcag caagcctccc aataattcgt gggcgatgtt cgcttcgggc aggtactcct
    41101 gcatcagctt gtaactgcgc ttgagcaggc tcgcctccaa tgaacctggt tttgcgtcat
    41161 accctgtatg ccaaatggcc gtactggctt tggatgtgcc catgccgaca tctgagttgg
    41221 cttcgagcaa tgcaatgttg agttgataac gggaaagctc ccgcgccatg gcacacccca
    41281 ctgcccccgc accgatgatg gcaatatcgt aagtcgttgt catagtgatt tcccagggca
    41341 ggcaacaggg tctgctgcct gctgtctatc atccatttcc gtgaaaagcc ttcactgcgt
    41401 ttagcgccct tccccactgc tccaatttct tttccctgtc ttcttttttc atctgtggct
    41461 ggtaggaagt ttccgatttc caattctgtg ccaatgcttc gaaacttgtc cccagggcag
    41521 atacaccggc caggttggca attccaatgg cagtggcttc cagcgccgca gaaacgcgca
    41581 cttcgatgtt caacaagtcc gcgatcattt gcatgaggta tggattcccc gatgggccgc
    41641 catccacttt gagatgcggc gggcgctgac cgaggtcttg agacatggcc gtaaccactt
    41701 cgtacacgcg gcaggcaatt ccatccaacg tggcgcggac aatgtcatcg gaggtggtgc
    41761 tgcggttcaa gccgaacatc gcaccttgta catcggtgcg ccagtgcggg gcggcgagtc
    41821 cttgcaaggc agggatcacc accacacctg catctttcga cctttgggca gcattgtggc
    41881 tggaagcaga atcggggatg aatttcaagt tatcgcgcag ccattgcacc gccgagcccg
    41941 tgacgaagat tccaccatca aaggcatagg ctgtctgccc gttgaaattc cagccaaccg
    42001 tggtgagtag tccattgttc gagagtttgg gtttgtcgcc gatgttcatc aacagaaaac
    42061 tgcccgtgcc gaaggagcat ttcatttcac ctgcgttgaa acatgcctga ccaaacaaag
    42121 cggcctgctg atcgaccaat annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42241 ngcccttttg cgaatctcgg gatggttttc gaacacccat ttgagtttgg gcgcagaaaa
    42301 gtaactgtcg agcaggagac ctgtcttttg gcggagcagg ttcgggtcca caccgggagc
    42361 gagcgcatca cagatggatt gtccgcgggt atcctgccac acgatggcgg gagttaccgc
    42421 ttcacccgtc tctgcatccc aaacaacgaa ggtctcaccc tggttatcaa aaccgaccgc
    42481 tgaaatatcg tacttgtcga gcaggggcgc acaagcctca attacagttc ggacaatatc
    42541 cgcgggctcc tgttccaccc agcctgcctg cggaaaacgc gcgggcaatt gagcagagtt
    42601 cttttcaagc atctccccca tttcattaac gatcactgct gttgtttgtg tagttccttg
    42661 gtcgataccg agcaggtact tcataagcat ctccttttat gacagccgat tcgtattttt
    42721 tgacttgcat ttccctttgt tttatgtcac aatatgtttg ttccagttag ggtgcccccc
    42781 tcaccctggc tggaacattt tttattcttt aactaaaaag ataagagcgg tatccaattg
    42841 gataccgctc ttgattttac ttcacgctga atggtttagt gttcgccaaa ttgcttttcg
    42901 atctcggcaa tcttggcctg gcgttccttg atcttgtcgc cttcggggac gccttcgaaa
    42961 cgattatctt caccaccggt cataaaggtg atgattgcgg ccagaacagc cacaccaatg
    43021 gtgatatagg aagttgtggc atctggaatg aggaagtgca tcacattttc ctcagagatg
    43081 gggatgaaac tcaatgccag ccctacaaca gccactacaa cgccgatcgg ggcggtcttc
    43141 ttagaccaga aacccaacaa ggcaacgaca aagacgatcg taaagtagaa gactttttca
    43201 ttgggcggga agaaaccgct gatcgccaat acaataccaa agatgacagc aagaacagca
    43261 tttgccttgg agaaaccacc aagattgaac ggaccttttt ccttccattt tggtccacct
    43321 tcagccatca agcccgcggc gatcggcatc accatggata ggtaaaggaa tacagcacag
    43381 gcgactgcca aaatcaggta agcgggagca tacaaagtgc tcgcgaacgc cagaatggct
    43441 gatacccaaa ctgctgtacc aggggtgcgg taaacagtgc ttaccttgga gagagcagcg
    43501 gaagccggca agccgccatc acgggcgaaa gcatacatca tgcgagaggt ggaagtgaga
    43561 ccagccaaag cacagacgta gttcacaagc accatgccaa cggcaaggaa gatgctcaac
    43621 caactcggca tgcgggatgc accccacata tagaagaatg atccccagcc tgcggcggcg
    43681 gcttcattca ggtctggcat cgtcagcaca taggctgcaa cagccaccaa gccaaannnn
    43741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttc attcaggtct ggcatcgtca
    43861 gcacataggc tgcaacagcc accaagccaa acacccagga ccagaataca gcggtccaca
    43921 tgcccttcgg cacgtttacc tgagcgtctt gggtttcttc agaggtgtgc gcagaagcat
    43981 caaaacctgt gatggtgtaa caaacgtagg aaagaccaag caggaaggca aaggccacgc
    44041 tttcagtgcg gaaaggaacc actgccccac ctgcatcgcc agtcacgttt tggaaggagg
    44101 taaggcggga gaaatccaaa gcaacagggc tgaaggcaaa gagggtgatg atcaaaatca
    44161 cggtcaagcc aaggatgaca taaccactcc agtcggttaa cttggtggtg atgtcaatgt
    44221 agtaatggtt caaaagcgcc tgagttccaa gggcaatgat caaaaagacc gtttggtgca
    44281 tggtgccaaa acctgaaaca tccacaccga gggcggtgcc gagcaagagg tctttgaaga
    44341 acacggtgta gagcagtacg tccaccgagg agaccacaca gatcaaacca atgagattaa
    44401 accaggcagt agcccaaccc cagaatttcc cgccgaggtg ggaactccag tgatacaaac
    44461 cgcccgccgt cgggaaggaa gatgcgatct gtcccatgcc aacagccacg ctcatggcaa
    44521 gaacaccacc gaccagccag atcaggaatg caccgcctga accaagggca ttgaaagcag
    44581 caggaaaagc agaaataccg ccagccaaaa tacagatgat tgaaaatgaa attgcaaagt
    44641 tcgagaagcc gctcatacgg cgtgaaagct cttgtgcata ccccagctta tgtagtacct
    44701 gtttgtcgtg cgcttgactt ttgttaccag ccattttcgt aattctcctt gaaatgattt
    44761 ttttacatca agaaaccgaa cttacgaaga gcagtttatt aactatcaac gatacacatc
    44821 ctcctcctcc ctttcgggag cttcctctga atggtatgtg gtgataacac tgacaatagg
    44881 acaaaggtac gaattaattc ctgccattat gacacaaaat gcctcttgat aaaatagacc
    44941 aaatgttcta aaatgaatat atggactctc ttgatacttt aaagttactc tcctcacaaa
    45001 tgactttcga gccagatgga gaaccacgca tcaactccga cactcccacc tgcttcagtc
    45061 cgaaagaaag ggatcacgcc tttactcatc ccgcccaact gccaaacgga cagaagatcg
    45121 ttctattgaa gacactgctg acctctgcgt gtgaacgcga ttgcttctat tgtccatttc
    45181 gtgcaggcag ggattttcgc cgtgccacgt ttaaacccga tgaattcgcc agccttttca
    45241 gtaaaatgaa tcggggcgga atggctgagg gtatatttct cagctccggg gtagcgggag
    45301 gcggtgttcg gactcaggat aaactcctcg atacggcaga tattctccgc aggaaatatc
    45361 aattcaaagg gtatctccat ctaaaaatga tgcctggctc ggaaaaagat caggtctatc
    45421 gcgccatgca gcttgcagac cgtgtttcgg tcaatcttga ggctcccaac acggaacggc
    45481 ttgccaaact tgccccccac aaggtcttta ttgaagaatt gcttcgccct ctgcgctggg
    45541 tggaggagat ccgcaggagc gtgcctgcct acaaattctg gaatggccgc tacccatcta
    45601 ccgtgacaca gttcgtggca ggcggttccg atgaaagtga ccttgagtta ttgaatacaa
    45661 caaactggtt gatgaagaat gtaaggctta agcgtgctta tttctctgca tttcatccca
    45721 ttcaggatac gccacttgag aataaacctg ctgttaaccc catgcgtgaa catcgtctct
    45781 atcaggcgtc gttcctcctg cgtgattacg ggtttgatct ggaagacctg ccttttacaa
    45841 aggacaacaa ccttccgatc catgccgatc ccaaacaggc ttgggcacaa atgaatctgc
    45901 tgcataatcc aaccgaaatc aacaaggctg ataaacatga attacttcgc atccccggta
    45961 ttggtttgaa gggtgcagat gccatccttt ctgcaagaag ggcgtatcgc ttgcgcgatc
    46021 tcgcatcact gaagaagctt ggcattattg ccgagcgtgc cgccccctat attcttctag
    46081 atggaagaaa atctgcatac caacttcgta ttttttaatt aggtcaaatc ggcaaaaaac
    46141 gggctgcaaa tggcacatgc catctgcagc ccgtttttac atgaaatcat ttaccccaga
    46201 gtcacagcca gtacatcata atacgtgccc gccccaaacc catcgaaaag gacattgtcg
    46261 ccaggtttga tgtttggcaa ttggcggaga aatgcgatca tattgcttgc cgcactgacg
    46321 tttccaaaat cgctcaagag ccacggcatg gggaattcaa tgccggcgct ttgtagttgt
    46381 ttcgctttca attgattgat cttgtaattg gcatggtgga caatgccaac tgcaatttgc
    46441 ttttccgggg taccgagttt tttcatcaac tcggcatatt cgtagaacac ctcagtcacc
    46501 actttaacac ccgtacggac aaacaacttg accattccca ttgggccagc cattgagatc
    46561 ggagagccgt cttttggttc atgcatcaaa ccagccacac ggatgtgcga cgggttttcc
    46621 tgtgtcactt cgatcaaaga atctcccaca tttttgccag ggtgcggata atacatatga
    46681 atggcaagga caccttcccc atcaaaggct tcatgattcc tgccaaccaa aaacttgaat
    46741 gtttcccccg ggataacccc aatcacacca gctgcattgg caaataattg cagggcatat
    46801 ttatcgtgtg aatggctggt gaaacggctc aatccttcaa ttccgcctac caaaaccttt
    46861 ttaccgagta gttcttctgc gatattgata tgtccctggc gcgtcagggc tgggtttagg
    46921 gccaaatgca aacccgccac agagctgtca caggctttgt gtacacttac cttcaatgcg
    46981 gtttccggga tgcctgctcg gcgacagatc tgttccacat aatccgggat gacaggtccg
    47041 cctgcaccga tcaacactgc atccacttcg ccaacttgcc agccatttgc gcgggcggcc
    47101 tcctgcagca ttcttgtgcc gatttgcatc tccaactcga tatcctcagg tttgtccaat
    47161 ggaggaatgt ggtgacggct taggaatccc agctctgcca gattcataat atcctcgtca
    47221 tccaagggct cgccatatct ttgcgccacc aaatcacgca ggctgtggtt gtcataactg
    47281 tcaccccatg cgccatatac cccgccgatg ccaagcgcag ggttgtacac cgtgctaaag
    47341 ccaaaaggta tacctgaagc tacaggaatg atctcctttt tgatatttgg aagttcaaaa
    47401 aatgatcgat gtttgttcat gtgattgata atctcctctc ttgcgcgtct ttaaagcttg
    47461 tctctataca gcaaacaaca cccatgacgc agttaagtta cttgtatgtt gaagaaaatt
    47521 tatttattca ttcgctgttg ttaccttaaa atattttgaa aacttttcac aaactcttgt
    47581 aattaatttt attgttgcta tactaaacaa caagtaaatg gaatatcaaa ggagatatac
    47641 aacatatgat gatgacaaca actacaacga tggaaacgct tgcaataaag actgaaaata
    47701 aattcaggac acttgtgacc aagcaatcct ataaaaaagg cgaaatgatt tgcgccatgc
    47761 cgagtgagaa catcacagat agaccgaacc gtttcacagt gcagatcggg cgggaggttc
    47821 atacgcatgt tgggaaactt gcggcattga atcactcatg tgaccccaat gttattttgg
    47881 acacgaagaa gatggtcatg gttgcccgcc gcgatattga aaaaggtgaa gagctttcct
    47941 tcttttaccc ctctacagaa tgggaaatgc aggcgccatt catttgcatt tgcggatcag
    48001 cgaactgcat ccacgttgta gcgggcgcgc gcttcctgcc gctctccaca cttgaaaatc
    48061 attaccttaa tccgcacatt cgtgaaatga tgatcgaatt acttaacaac acccaggaac
    48121 acctcaagag tttgaagtaa ttctcagaac aaacctgaac ataaaagaag aggacatcgt
    48181 ctactgcgat gtcctcttct tttatgaaaa actataattt tcggtgtaaa gccagcaggg
    48241 cgtaggcatt ttcccagggc ttgccaagcc ggtggttgtc tgaaacttcg attttattga
    48301 gtttcattgc tatttgcggc aaaagcgatg caatggtagc ccagtcatca cccagcacgg
    48361 cagacttcat tttatccgca agctgccccg tccacctcca ttccatgcgg aatttatcgg
    48421 cttttaccca atagtcccgc ttctcccagg ctgccactga ggcatccacc ccatctgaga
    48481 tggattgcag cgccagtgca ataaatgaag ccatatcctt cgcctcaggc gtcacttgag
    48541 acattttcat catttcacgg atgcatagca cgatggattt tgtcaaacgg gttcgttctt
    48601 ttcctacaga atcggggttg attacacggc tcaaggcaat ttctccagtt gttttttgac
    48661 acaagcgcgg gattataccg ctgatttcag ggggatggac aaaaacaaaa tttgttgacg
    48721 caccctctcc cctgctttat aattcggccc gctcaatatt tgagttgggg acgtgttgga
    48781 attggcagac aagcatgact taggatcatg tgccgaaagg catgtgggtt cgactcccac
    48841 cgtcctcact attttacaag acccgtcgta agtgctttta tccagggtct ggaggaaact
    48901 ttgaatatcg aaaaacaaat tcaggaagac cattcagcca agctcatcgt tgatgttgag
    48961 caggaaaaaa tggatgttta caaacgccgt gcagctacca agatttcggc gcgcagcaag
    49021 attcccgggt ttcgcccggg taaggctcca tacgatcttg ttgtaagaac ctttggtgaa
    49081 ggtgctgtca ccgagcaggc cattgacctg tttattgatg ccgaatatgg aaatcttctc
    49141 aaggaagcgg atgtaaatcc tggtgcagct ggttcgcttg aatcgatcga cagcctcacg
    49201 ccccccaagt taactttccg cgtaccgctt gctccggaag tggacctcgg cacctatcat
    49261 gatatccgca tggaatacga atggcaggct cccgatcagg cggcagttga tgccgctttg
    49321 gaagacctgc gccagatgta tgcatccact gaaacggttg accgcgccat tgaactcggc
    49381 gattatgtgc ttgtcgatgt gaattcggaa accgctgaag taacccgcaa gggatttgcc
    49441 acctttgtcc gcaaggaaga ccgcgacacc gaatggccct acaacggttt tgcaaaagag
    49501 ctcattgggc tgaaggctgg ggagagcaag accatccaac acaccttccc tgctgattgg
    49561 gaattggaag agcttcgcga caaggaagtg acgatggaag tcacggtcaa gactgtgcgc
    49621 ggtgtaaccc tgcccgatat cgatgatgcg tttgcaaaga tgaccggcgc tggcgaaacc
    49681 gtagatgagt tgaaggaagc tgttaagaag gacgtggaag cccgttcaca ggccgagtat
    49741 gatgacaagt acttcgtaga gttgatcgaa aagatcaaag aagttgccac cattaaatac
    49801 cacgaacaca ccctcgaaca cgaaggggag catgttcttt cagatctgag caatcgcctg
    49861 gctcaacaga acatggatct tgatacctac ttcaagatgc gcggaaccac ccgtgaacaa
    49921 ttcattcaag atgaagtgaa ccccgtggca aagaagcgcc ttgagcgcgg attgatcctt
    49981 gatgagatcg ttcgtcagga aaagattcag atcgacaacg aggctctcga agctgaatac
    50041 aacgttacgc tcaatggtct tgcccaacaa ggcatggact ttagcaagat tcgcggcggc
    50101 agaaaaggcc aaaagcagtt gagcgaagcc attgcaatgg aatccgccag ccgtgtgatg
    50161 acccgcaaag cattggatat gatcaagtcc attgccaccg gggagtataa gccgattgaa
    50221 gagagacagg ctgaggcgag tcctgaagcg ggcgaagctt ctgaaacaga gtcaaaacct
    50281 gagtaaacct ttcaagggcg ggttaataat ccgcccttga aattttaata aaaatccaat
    50341 acacgctgtg tatgattgtc ccaatcaaaa aggagacaaa aatgattcca gactttaaga
    50401 atgtagttcc aatggttgtt gaaaataccg gccgcggcga gcgcgcttat gacatttact
    50461 cgcttttgtt gaaggagcgc attgtcttcg tgggaacacc cattgacgac caggttgcca
    50521 atgtgatcgt ggcccagttg cttttcttga accatgaaga tcctgaaaag gaaattcgca
    50581 tgtacatcaa ctcgcctggt ggtcagatct atgcaggtct tgccatttac gacacgatgc
    50641 agatcatctc gaacccgatc agcactgtgg ctgtaggcgt gaccgcttct ttcgggaccg
    50701 tactgctgac cgctggtacg aagggccgcc gttacgctct gccccatgcc accgtccaca
    50761 tgcatcagcc tcttggcggt gcgcaaggac aggccaccga tattgagatt caggccaaac
    50821 agatcctgcg cttgaaggct ttgttgaatg gtgttctcag cagccacact ggccagccgc
    50881 ttgaagtgat cgaacgcgat gcagaccgcg atttttacct tgatgcccag caggctgtgg
    50941 attacggctt gatcgatcag gtcattgaga tgccagtgaa gagcaaataa aaaaataagc
    51001 acgtaaacat taaaaggcct cgaggagaaa tcttcgaggc cttgcgttta taatatcgtc
    51061 acaacccact atttttgaag tttagactcg ctaaacactt taaatcaaaa atcagaaact
    51121 ggaaattgcc atgacttttt caattgttgc atgtgatctg aaagaaaaga cctgggggat
    51181 tgccgtagcc tcaaaatttc ctgcggtagg cgctgtggtt ccatgggcgg cggctggtgc
    51241 tggagcggtt gccactcaat cgtttgcaaa tacttccttc ggcccacgcg gactggaaat
    51301 gatggctgca ggcctttcag cggatgaaac actggctaaa ctgctgatgg atgactccga
    51361 tcgtgaattg cgccaggtcg gtctggtgga tgcaaacggc aaatctgcaa cctacacagg
    51421 cagtggatgt tttccgtggg caggcggggt caacgggaag ggctatgcca ttcaaggaaa
    51481 tatccttgcg aatgcgaagg tggtgcctgc gatggaaaag aagttcctcg ctacaaaagg
    51541 tgacttgccg acacgcctcc atgccgcgct tttggctggt gaccgcgccg gtggtgacaa
    51601 acgcggacgg cagtctgctg ccatttatgt tgtgaagccg aatggtggct acggcggcta
    51661 cgtagaccgt tggattgatt accgcgtaga tgaccacgaa gatcctgttc cacgtttggg
    51721 cgaattgctt gaactgcacc gtctgtattt tgataaaagc cctgaaaacg agcgtgccgc
    51781 cttgaaaggc aaaactctgg agcaaatgac gtccattttg aaacgtgcag ggtatctatt
    51841 aaagaaaaac accgcttttg tagatgcatt caacgctttt atcggaaacg aaaacttcga
    51901 ggaacgtgcc gacccgaacg gtcaatggat tgacaagcct gtgctgaaat atctagtgaa
    51961 gaagtttggg aagtaaacag cgtatggcgc tggaaaatcg caaatcaaaa tcataaaata
    52021 aaaaaacact catgatccct tcaaatataa aagctctcat tcttgatatg gatggcgtca
    52081 tctggaaagc cgatgcgccc atcggcgact tgccatccac gttcaaccgc atccgcgagc
    52141 gcggattgaa attcgtcttc gcaaccaata acggcacaaa aacacctgag gattatcagc
    52201 aaaaactggc cgacctgggt gtggatatag ccccctcaca agtggtgact tcagccatgg
    52261 gcatcgcgtt catgcttgcc cagaaatttc cacgtgggac gaagatcttt atgatcggcg
    52321 aagatggcat ccgcgtggct ttggaggaaa aaggattcga gatcctcagc gcagaaaatg
    52381 cttcacaggc gcaagccttt gtgatgggca tagaccgtgg aatcaacttc caaaaagttg
    52441 cggaagcaac tctgcttgtc cgcgctggaa ttccctttta caccaccaat accgacaaga
    52501 cctttcccac tccacgtgga gaaattccag ggtcaggcgc ctgggtctcg gtcattcaaa
    52561 cagccacgaa cgtagaaccg atcatcgcgg gcaaaccgtt tccattcctg atggaactct
    52621 ctctcgaaaa acttggtacg aaaaaggaag agaccctcgt tgtcggtgac aggcttgaaa
    52681 cggatatcgc cgctggtcaa agtgtaggct gccctactgc gttggtattg agtggagttt
    52741 caacaaaggc acaagctgac gcgtggaccc caaaaatgga tatcatcgca gcaagcctgg
    52801 ccgacttggt gaagtaaaag aaaatatgct tgtctgaagg caaattacat aatcaagatt
    52861 ctaaaagaac ggcatcgcag gacacttcct gtgatgccgt tcttttttac taactactat
    52921 cttgttgctt aattcggata cccacccgga gtctttgttg gtgtaggcac tgtaccgatc
    52981 ttgataacca ccgtcagaga atcgccgaag gcgaccgact tatcgtttga aaggcgccag
    53041 gtgcctttga tctcaccaga ttttgctgga gcggtcaagt tcacagagat gtccacgctt
    53101 tcaccaggct ttacttcctt gccgatcggg gtggattgtc cattcatgcc atcccctgca
    53161 acaaaaacaa gtttgtatgt cgcggtccag gtacaggcgc cggtattggt cactttccag
    53221 gttttggtga aggtttggcc tgctgtcatt gcagtcccat ctggtacggt cacatcagaa
    53281 acataaatgg acatatcgca tccactggta gggacagctt ttgtggccgt ggcaaggatc
    53341 gtcagcggtt gggtggtcac agttgccaac ggagcaaccg tgggggaagg aacaagcaag
    53401 gctgtattca gggttgccgt tggggttaca gaaagcgcga tcgcggtctg ccctgcattc
    53461 actgtagctg ctgcattggt gtagatcaca ttgatgtcac cctcggaagc ctgttcactt
    53521 tttccgcctc tgaaggcgta caccagtgta cagatcccag ccagaaccag aacagccaca
    53581 agtccccaca gaattgctgg cctagatttc aaagcattta tatcgaaatt tttcaaattc
    53641 attcaattct cctttttgac aaggcacaaa ttatagtgtg ggtaaattaa aagaaattaa
    53701 tcgtttgacc tgcgaattcc ctacttttca catactcaac gagcataccg aatatttcgg
    53761 cccaaaagat gcaaaaagaa tggcatcaac catacggtag agtaaaatca ggaaatgcta
    53821 atttatgatt tcgaccaaaa aacacttcta gacctcctca cccgctgggg tgaacctgct
    53881 taccgggcca aacaaatctg gcagggacta taccaacatc tctataattc acctgagcaa
    53941 ttcaccaatc tgtccaagcc gttgcgcgat aaactcgccg aagggttgaa ctttgcgccg
    54001 cttaagccga agctttacct cgattcgtcc gatggacaaa cacgcaagac tcttttcgag
    54061 ttaggggatg gtcaagtgat tgaatccgtt ttgatgcgct acggtgaccc tgccgacaac
    54121 cacgacacca gcggaagaga tatccgctca cgaaggacgt tgtgtatttc cacacaggca
    54181 gggtgtgcca tgggatgcgt cttttgtgcc acaggtcaaa tggggttcaa acgccaccta
    54241 agcagcggtg aaatcgtggc ccaggtgatg tactatgccc aaatgctcaa agaggagaac
    54301 ctttcggtca cgaacatcgt ctttatgggc atgggtgaac cgttccacaa ctacgaaaat
    54361 atgatggccg caattgaccg cctcaacgat gcggacggat tcaactttgg cgcgcgtcga
    54421 atgaccattt ccacggttgg tctggccccg caaatcaaac gctttgccga cgaacacaga
    54481 caggtgaatc ttgcgatttc ccttcatgcc gccgatgatg atgagcggct tgccattatg
    54541 ccagtcaaca agcggtacaa gatcgccgat gtaatggaag cctgcaaata ttatgtggaa
    54601 aaaacgcatc gccgcgtgac ctttgagtgg gctctgatca acggagtcaa cgacaccccg
    54661 gaaacagcca aaaaactcgc cgccagactt agaggtctgt tatgccatgt caacgccatt
    54721 cccctcaacc ccactactgg atatgagggc gaagcgacct cacgtgaaag ggcgaacctg
    54781 ttcaaagaat cgctggagca ggctggggtt ccgtgtacga ttcgtatgcg ccgcggcatt
    54841 gatattgcgg caggttgcgg tcagcttgct ggttctgtta attagaaaaa tttctattgc
    54901 tgattacgag agtaataaaa gtagggatgt tatcttggct acacgaaatt tcaaagaatt
    54961 aacttccgct ttaacttaaa cgcatatcat ttcttgtcag aacaatttga ttgatgtata
    55021 gtgatgcaac ttttgactta ataaggtgac gctcaatgga acaaaaacaa aactggctcg
    55081 aacgccccat tcatcctgca ttaccaggcc ttacaaatga gatcgcgatt tttattggcg
    55141 tgattctgct tgccataatc acaaggtttt acgaccttgg cacacgcgta atgagtcacg
    55201 atgaaagcct gcacacctat ttctcatggt tactctatcg cgggcagggc tacgaacatt
    55261 cacccatgat gcatgggccg tttcaatttc acattgtcgc attaacttat tttctgttcg
    55321 gtgcgagtga cttcacttcc cgtattcctg cggcattatt cagcatagcc attgtagtaa
    55381 tggtctggta ttggcggcga tatcttggta aatggggtgc attgattgca ggtcttttgc
    55441 tggtcatttc cccttacatg ttgtattacg ggcgctacgt ccgcaacgag tcgtttgtag
    55501 cattctcggg aattgtgatg ctatatgcca tgttgcgcca ttttgaaaca ggcggaaaaa
    55561 aatatttact gatcatggca ggcgcccttg ccttgcattt cacatcgaaa gaaacttcgt
    55621 tcatctacac ggctgaagcg ctgatttatc tggccgttta ttttattgtc caggtaacac
    55681 gccgcccatg gggcgaagcg gagcgcgatt accgctcctt tatcatttcg cttggggttg
    55741 ctgttttact ggctggtgct gctgttgcat ttggattgta ctcacgtgac gcagccacca
    55801 tctctgcgac agagacggcc atgccaagcg accctgccct gcaggcaacg cctccaaccg
    55861 aacctgaaac tggtgctttt tccccaagct tgattctcgc cgcagcagcc atcgctgcac
    55921 ttgtagcggc tggtgttttc cttattcgcg gttacacatg ggatcgtatt cgtaacgaac
    55981 ggtcttttga aatgctcatg gtggcaggga cgatcgttct ccccatgctc actccatttg
    56041 cgatcaaagg gctggagcgc tggcttcacg tcagcatccc tacaactgcc cctgaagtgc
    56101 aagccttaag tggagacata aagtcagtac tgaccatcgg cgggcttttg atcttgatgt
    56161 ttatcatatc cactgtcatt ggcttgctgt ggaacaagga aaaatggtgg aagatcgccc
    56221 tcgtattttg ggttccattc accatacttt acaccaccgt ttttaccaac agcgacggct
    56281 tctttacagg caccattggc tcgctcggct attggattgt ccagcaggat gtccaacgtg
    56341 gcagccaacc ctggtattac taccttctga ttcaaatccc agtgtatgaa tttctacctg
    56401 caatcggctt cctgcttgct cttgtgctcg gcttccgcag aaagatcacg accagcatcg
    56461 aagaagatgt ggtcgatcag aacgaggaat ttgtcaacct gctaaaacaa gagcacgaag
    56521 gagaagaaaa cacagaggaa aaaaactttt caaatacatt cagcctgctt gtttggtgga
    56581 gcattatcag tttgatcgct tttacctatg caggcgaaag aatgccctgg ctcaccgtcc
    56641 acatcacact gccgatgatt cttattacag gctgggcgtt gggccacatc attgacaccg
    56701 caaattggga aaagatccaa agccaacgcg ttccattaac tctcgccgca ctgaccgtct
    56761 ttatcgtcag cgtcatgggt atggtacttg ctctgaacag ccccactccc ccattccaag
    56821 gcaaggagtt ggagcaattg caagccacca gcgctttcct gctgccaacc attatgacac
    56881 tactcagcgc tgtgggtctc acttacttgt ttaatggctg gaccttccat gaagcgaagc
    56941 gagcttttgt gttgcttgta tttgcattcc ttgcagtact gactggccgt gcagcatttc
    57001 gatcttctta tatcacttac gaccaggcaa cagaatttct tgtctacgcg catggagcta
    57061 ctggaataaa agagatcatg caacaagcgg aggaaatttc cagacgcacc acaggcggca
    57121 tgggagtagc ccttgcctac gacgccagtg ctcccaacac cggtgtttca tggcctttcg
    57181 tttggtatct ccgtgattac accaatcaac gctcgttcga ccagccaacc cgcgccttgc
    57241 gcgactcagt tgtggttatc gtcgatgaaa agaatttcga taagatcgag ccagctctcg
    57301 gcccgggtta ttaccgcgtg gattacatcc gcatgtggtg gccgatgcaa gactatttca
    57361 atctggtgga tgagcgtgat cccactcaac cattccctga agattacgcg tgcagcggag
    57421 cgttgagctt tctaaaactt gttaaatcaa aagattacac aacattttgt gaagggttca
    57481 ccaacccaca gatccgtgca ggtatcattc agatctggct taatcgtgac tacactcaat
    57541 atgccgaagc caaaggccgt acaaacctga ctctggccac ctgggacccc gctgcaaaaa
    57601 tgcgccttta cattcggcag gacgtcgccg ctcagatatg gaactatggc ctggcgcctt
    57661 ctgcagttgt acctactgaa gatccgactg aaggaaaata tattccgctt tcatcagatc
    57721 tgatctttga aacctttgaa aagacacctt tgaatgcgcc tcgttcgctg gcttttgcca
    57781 aggatggctc gatgtatgtg gccgactcgc gaaaccatcg tatcgtccat atcagtccat
    57841 tgggcaaggt tattaatgaa tggggaacct tcgccgatgg cgcgagcgtt ccagttggtg
    57901 atggaacatt caacgaaccc tggggtgttg ccgttggacc tgacggctcg gtatacgtta
    57961 cggacacatg gaaccatcgc attgaaaaat tcagcgccga cgggaagttt attaaagctt
    58021 ggggcttctt tggtcaggga gaaacacccg aagctttcta cggcccgcgc ggattggcgg
    58081 tggatgctca agggcgagtc tatgtcacgg ataccgggaa caagcgtgtg gtcgtttttg
    58141 atgcggatgg taatttcatc actcaatttg gttcagcagg tctggatgca ggtcaatttg
    58201 atgaaccagt gggcatcaca gtggataaga atggaacggt ctatgtcgtt gatacatgga
    58261 atcaacgcat ccaaaccttc actgctgtgg aaaccgcagg cgcacttaca ttcctcccag
    58321 ccaagcaatg ggacgtatac ggctggtttg gtcagtctgt agacaataaa ccctttattg
    58381 cagtgaacaa tgcatttcat gtcttcatca cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    58501 nnnnnnnnnn ntcttgcgag gatttttttc tggtgtataa tcgcgatgct ttataaaaaa
    58561 acatcccccg agggagaata atgaaactcc acgaatacca atcaaagaca attttttcaa
    58621 agtatggcat cccgattcca aagggaaggg ttgcggctac cgctcaggaa gcaagacaga
    58681 ttgctgaaga gttgggtggc cgcgttgtca ttaaatcaca agtactggtc ggcggacgcg
    58741 gtaaggcggg cggcgtaaag cttgccaaag actctgccga ggcggaacaa ctcgccactc
    58801 agattttagg catggagatc aaaggcctgc ctgttcgcaa agtccttgtt gatgaagcct
    58861 ctgccattga tcaggaaatt tattttgcca tcaccgacga ccgggcagcc aagaaacccg
    58921 ttatgattgc ttctgcagca ggcggggttg acattgaaga agtagctgcc aagactcctg
    58981 aaaagatcat caaggtcaat attgacccgc tgttgggatt gcgcgaatat caagcccgtg
    59041 acgtagccgt cgccattgac ctgccgcgcg aacactggaa ggatttcgtc aagatcgcca
    59101 tggggttgtg ggaagtttac aagaactcag atgcatctct ggctgagatc aacccacttg
    59161 tgatcaccaa agacaaaaag ctgattgcgc ttgacggaaa gatgatgatc gacgacaacg
    59221 cccttttccg ccagcccaat ctgagcgaac tgcgcgacac agatgaagat gccccttccg
    59281 agatcgaagc tcgcaaatac ggcctctcct tcatcaaact tgacggcaac attggatgca
    59341 tggtcaacgg cgccggcctg gccatgacca gcatggatgt gatcaaactt tttggcggcg
    59401 aaccagccaa cttccttgat gtaggcggcg gtgcaggtgc tgaaaaagtg gcagctgcca
    59461 tgcgcatcat cctcaccgac ccgaatgtga aagccgtact cttcaatatc ttcggcggca
    59521 tcacccgctg tgatgaagtt gcccgcggta ttctcgctgc aatggacgaa gtcaaaccaa
    59581 aagttccaat ggttgtccgc cttgttggca cgaacgccga agaaggtcgt cgcctgcttg
    59641 aaaatgcaaa tatgatcacc gcagaaaccc tcgccgacgc ggcgaagaaa tctgttgaag
    59701 ccgcgaagaa agggaattaa tccatgagca ttttagtaga caagaacaca cgcctgatcg
    59761 tgcaagggat cacaggcaac gaaggtttat tccacacaac tcagatggtt gcctatggca
    59821 ccaatgtggt gggcggcgtc acccctggta aaggcggtga atgggtgctc gacggaaaac
    59881 tccccgtttt cgattcggtc aaaactgcca aagaagccac cgacgccaat gccaccatca
    59941 tctttgtacc cgcccgtttc gccccagatg ccatgtttga ggccgcagat gctggcatcc
    60001 cccttgtcat ctgcatcact gaaggtgtag ccgttcagga catgatgcgt gttcgcaatt
    60061 accttgacca gaaaaaggtc cgcttgatcg gccccaactg ccctggctta ttaactcccg
    60121 gtgaagccaa aatcggcatc atccccggca atatcgccat ccccggcaac attggcgtgg
    60181 tttcccgctc tggcaccctc acctacgaag tgttgtatgc catgaagaac cttggcatgg
    60241 gcgccaccac ctgcgttggt atcggtggtg accctgtcaa cggcaccaac ttcctcgatg
    60301 tgcttgaaat gttcgaacat gaccccaaga ccgaaaaggt catcatgatc gg
//
LOCUS       DAOQLC010000007        60048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_108_length_60048_cov_25.013318, whole genome shotgun sequence.
ACCESSION   DAOQLC010000007 DAOQLC010000000
VERSION     DAOQLC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 60048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_60048_cov_25.013318"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..705)
                     /locus_tag="PL000_02465"
     CDS             complement(190..705)
                     /locus_tag="PL000_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87786.1"
                     /translation="MKKTRNTKGTLETLLVIGLILATLTACESKPLEVTDISVHPDPV
                     IGQTATLRVEIMSINDEKDVTINITLPDGVKLMSGDLEWKGSLIANQPQAHELELCIL
                     YEGEWGFFISTYSQYAPTSRYGDSEILYIITSADTVRVVPSRKYHVTQPPGGMVYPTP
                     VPQIPPADICL"
     gene            complement(785..1324)
                     /locus_tag="PL000_02470"
     CDS             complement(785..1324)
                     /locus_tag="PL000_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87787.1"
                     /translation="MKKINTQKRMASLFLFIGLTVSACSSSGGYPYPLNITSVKVFPE
                     PIVGEVVTLEVEIVSVDDKANVKFTIDTLEDTGNKIHLVSGEPAWQGTLTANQPKIFQ
                     VKICVMEEGSWPVRLYTALPSSDQEGGYDFEIIQLESTLDSGKLIPSSEYTFSQEEYA
                     NRPTPRPIAVSSECSGISK"
     gene            complement(1636..2502)
                     /locus_tag="PL000_02475"
     CDS             complement(1636..2502)
                     /locus_tag="PL000_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC87788.1"
                     /translation="MAQKTGLGKGLDALIPAGGKTPPASSSGGGTGGVQQVAVEAIKP
                     NPRQPRVHFKEDELAELAASIREHGVIQPIIVTPKADGTYLIIAGERRWQASQKAGLR
                     TVPVITRQANNQELLELALIENVQRADLNSIEEAEAYRQLVEEFGLSHEAVAKRVGKS
                     RVAVTNTLRLLGLADVVKQALVDGTVTEGHARALLALSTQKAQASALQTIINLSFSVR
                     QTEEYVRKLAGQKPIKAKKPNRSADVADVEKRLQNSLGTKVALKHSKKGGTVTIYYYS
                     GEELDALLEKLI"
     gene            complement(2581..3351)
                     /locus_tag="PL000_02480"
     CDS             complement(2581..3351)
                     /locus_tag="PL000_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC87789.1"
                     /translation="MTRIYTLVNQKGGVGKTTTTINLGAYLAQLGQRVLVVDLDPQAN
                     ATSCLGVDKLSVQGGTYEALLGDTSIASYVLLNEKLKLSLLPSSPSLAGAEVELVDEL
                     AREVRLRRALEQVASQYDYVLIDCPPSLGLLTVNGLMAAINGVIVPVQCEYLALEGLG
                     QLTQTINRVRSLLFPELNVRGVVLTMFDNRTNLSTDVVSEVNRHFPDQVFKSVIPRSI
                     RLAEAPSYGLPISAYAPTSVGAAAYEALAKELLKGDKN"
     gene            complement(3348..4199)
                     /locus_tag="PL000_02485"
     CDS             complement(3348..4199)
                     /locus_tag="PL000_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC87790.1"
                     /translation="MLELVSLQPVDYLVVGHAAVDITPTGMQLGGTVSYSSLTARALG
                     MRVGIVTAVGEGAPMQALDGITIVNIPSEHSTTFENIKTDHGRRQVLHHQAAPITLEH
                     IPQVWRSAPIIHLAPIARELDPSLPEKLAGSLLGITPQGWMRQWDENGQVTASAWENR
                     DQALRYAGAVVLSVEDINRDLELVEEMAHQTRILCMTEGELGSVLHWNGDRRRFRPSV
                     MHEVDATGAGDIFAAAFFIRLYATRDPWEAARFATQLAARSVTRVGLNGIPTKDEIEE
                     CLMEVYQ"
     gene            complement(4300..5139)
                     /gene="jag"
                     /locus_tag="PL000_02490"
     CDS             complement(4300..5139)
                     /gene="jag"
                     /locus_tag="PL000_02490"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNC87791.1"
                     /translation="MSGTTLEIIAPTVEEALAQGLAQLGLTADAVSVEVLDSGSKGLF
                     GLGGRQVRVRLTVNPPPVEETHTPAPARQKPADRPKGKPAPAQKAESAPKAQREPKPR
                     TEKKPAPEAPAPVKQRPLSEGSDALLDTTESVISKLIHHLGMKAQVSAHYDESSTDDR
                     RVIQVDVRGDDLSALIGRQAETLNAFQYVASLIVGKETQKWVQLVVDVEGYRSRREKQ
                     VRQIALRMVDQVIKTGRKATLEPMTASERRAIHIELRGHPAVTTESVGEEPHRKVVIL
                     PKE"
     gene            complement(5156..5947)
                     /locus_tag="PL000_02495"
     CDS             complement(5156..5947)
                     /locus_tag="PL000_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNC87792.1"
                     /translation="MWDTIIINPMTNLLLWIYDVLGHGPHMFGLAIILFTILIKAVTW
                     PLNASQVKGAQAMQELNNDKEWQEIQKKYAKDREKLAQEQMRIYKEKGINPFASCLPT
                     LIQFPIIIGLYQSITRALSITPFDMLKLARTIYPFQNVENIIPLNSTFLWMDLGRPEG
                     IFQSIIHTSFLPNGFPLLALIVALTTYVQSKLTMPTSSNPNDQSAQMSSMMSIYMPLL
                     LGWFALNFASGIAIYFITSNLLGIIQYAATGRANWSNLLPGSKNK"
     gene            complement(5960..7033)
                     /locus_tag="PL000_02500"
     CDS             complement(5960..7033)
                     /locus_tag="PL000_02500"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC87793.1"
                     /translation="MNTKRLVLISLLVLGSLILSACSGNRTVSNNWHGLAADAEHAYV
                     ASGTHIYAVNMDNGDEAWRFPQEADSKLMFYANPVLTADGQLLIGSAGSSHPFYSLNP
                     ANGKENWVEPFTGAKGAWLASPLIANDTIYAPNTDGYVYVLDLEGKQAADPIQIGGAL
                     WSAPVTDGKFIYIASLDHHLHIIDPSDGSTVDTIDLGGAVPGTPAVAENGVYVGSFAS
                     TVEFIQSNGNHEVVAKTENWIWGSPVLAGETLYYADLTGKVFSFNTTEGSQNWEIALP
                     DAVVAGLLVSGNQIYVATEAGNLIALDLDGNTVWEKPVGGKLYTAPVLSGETILVAPY
                     QAETTLVAYDVDGKQVWPFTPEK"
     gene            complement(7074..7376)
                     /gene="yidD"
                     /locus_tag="PL000_02505"
     CDS             complement(7074..7376)
                     /gene="yidD"
                     /locus_tag="PL000_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNC87794.1"
                     /translation="MNPEPEREFHLHDYSHEPRLRDMPRNLANWPRIAVLALIRLYQM
                     AISPGLPANTCRFYPSCSHYGYQAVYKHGALKGSLMAVWRVLRCNPFNPGGYDPVP"
     gene            complement(7366..7719)
                     /gene="rnpA"
                     /locus_tag="PL000_02510"
     CDS             complement(7366..7719)
                     /gene="rnpA"
                     /locus_tag="PL000_02510"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNC87795.1"
                     /translation="MQSKFRLSRSEDFKRVRRTGKSYAHPLVVLVAQTTESPARHVRV
                     GVAAGKTTGTAVHRNRAKRLLREAMRPLLTSLASGWDLVLIARPSLVSATLEDTRSAL
                     LALLQRAKIIPTDES"
     gene            complement(7764..7934)
                     /gene="rpmH"
                     /locus_tag="PL000_02515"
     CDS             complement(7764..7934)
                     /gene="rpmH"
                     /locus_tag="PL000_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC87796.1"
                     /translation="MTKRTYQPKIRRRVRVHGFRARMATADGRAVLKRRRLKGRYKLT
                     VKANEHVKKTRW"
     gene            8183..9610
                     /locus_tag="PL000_02520"
     CDS             8183..9610
                     /locus_tag="PL000_02520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC87797.1"
                     /translation="MSNQVEHYKSLFEYAPISLWEEDYSGIKLFFEGLRLGGVTDLKA
                     HFAHHPNDVDACMRRIKVTHVNLKTLEMFGARSVEELIANLHKIFRDEMRHHFQTELL
                     ALWNGETNWSGEGINYTLKDKPLNIRLRWKILPECCEKWECVMVAIEDITPLKQAEKR
                     FQNLFTYAPISLWEENYQGIREMFDTLRAQGVTDLKTHLLQTPELVGQFMNKIAVTDI
                     NLKTLELYGASSKEELLANLDRVFRDEMSAHFADELFDMWSGKTTYEREGINYSLSGE
                     PFNVHLDWRLMPGHEDDFKWVMVAVQDITARKKAEEYMRYLGTHDVMTGLYNRAFFED
                     ELLQLASSESEPSFIMIDLDGLKHVNDTLGHQAGDNLIRRAAEVLKTAFAEKATIARI
                     GGDEFAIILKNPEAPQEYLDGIHALVALNNKYYQGPQLQLSMGYATRRKNETLEKTLI
                     RADEAMYQNKESNYKNHPSWQRRKE"
     gene            complement(9670..10683)
                     /gene="add"
                     /locus_tag="PL000_02525"
     CDS             complement(9670..10683)
                     /gene="add"
                     /locus_tag="PL000_02525"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNC87798.1"
                     /translation="MKNFDPKYNNIPKTEIHCHLEGAIRTQTIIDVAREYDLSLPSYD
                     VRELDTHVKVFDQMRNLEAVLEAFGIFQNSITSPAVFERISWELFEDCAKQNIKLFEV
                     RFSPDWAFHGHNLDWDACLDGLLRAKERAEKEFDMAIGIIAITSRGMGAESCVKTVDW
                     AIRHKKHIQAVDLADGELLYPMSDFVKPILKAKEAGLKVTIHSGEDTPASAVWDTIHN
                     FQPDRIGHGIHSIEDMKVVDLIKERGITLEVNPWSNYLTNSVRTIEEHPLKKLFDLGV
                     RVTINSDDPEVLETNVNNEYRIAHEVLGMSMEDIATCNRYAFESSFVDEKVKQRIWQK
                     YFM"
     gene            complement(10693..11940)
                     /locus_tag="PL000_02530"
     CDS             complement(10693..11940)
                     /locus_tag="PL000_02530"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HNC87799.1"
                     /translation="MTNSFSLLRITPDRMLDTFNQLALIGSTGDGGVNRPTFSEAHLA
                     ARKWFKEQIEDSGLEFRTDGAGNHSAFLTCQRSNVPTLLLGSHLDSVPKGGRFDGALG
                     VMAALEVLRTVKESRINLRLNLEAIDFTDEEGTHLSLFGSSAISGHLHPEDLKNPYSG
                     RENFTAGLARAGLTEASLFQAKRAKESLAGYLELHIEQGKRLERAKIDIGIVSAIVGY
                     GSYRMSYIGRADHAGSTTMEDRLDAGQGVAAFILAVRETVMRDFPNCVANVGKIEFAP
                     GASNIVPARADFLLEFRSPDENQLAELDAALMTLAKEMAEKFGLQLVAEPLGKHLPNL
                     MSTEIQDAFKNSCDDLGLSSTVLASGAIHDAQSLVDVCPIGMIFVPSVDGASHAPREF
                     TKWADCVNGANVLLQTVLRLAAG"
     gene            complement(12039..12482)
                     /locus_tag="PL000_02535"
     CDS             complement(12039..12482)
                     /locus_tag="PL000_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC87800.1"
                     /translation="MSNLIRWEPAREMMTLREAMDRLFDDAFTRPLSIAGNGWAVPAV
                     DMYQTDNEVVVKAALPGMKADDVQLNVTGEVLTIKGEIKQKDELKEKAYHLREQRWGS
                     FERSVILPTDVVADKAKADFENGILTITLPKAEEAKPKSISIKAK"
     gene            complement(12708..13427)
                     /locus_tag="PL000_02540"
     CDS             complement(12708..13427)
                     /locus_tag="PL000_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC87801.1"
                     /translation="MSDSLERRRILVVDDEERMVRFIRMNLEHDGFQVSEAFNGKQAI
                     QKLRDVTPDLILLDVMMPDLDGFEVLETIREITTVPVIMLTAKGEEDDRVRGLEGGAD
                     DYVTKPFSPRELVSRVKAVLRRTEGATGSMHGLLEIDKRLKIDFDRREIWLEGKLVKL
                     RPTEYRLLFHLVQNAGWVVSHDQLLQKVWGYEYRDEPHYVRLYINYLRQKLEKDPADP
                     KYILTERGVGYRFVDYKRSKE"
     gene            complement(13435..13677)
                     /locus_tag="PL000_02545"
     CDS             complement(13435..13677)
                     /locus_tag="PL000_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87802.1"
                     /translation="MSYTVILHISGETSVAGEIEELPKPTDTIIMVMNPRQKDGKDLH
                     YIDNNVTKVIWPLAKLSFIEILEGTGEEKIISFVRE"
     gene            complement(13698..14222)
                     /locus_tag="PL000_02550"
     CDS             complement(13698..14222)
                     /locus_tag="PL000_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC87803.1"
                     /translation="MIICSNCKNTNLDGAMFCAECGSQLVGKDTLITQNISTKQFEKA
                     RESKPARPNDTYQPFDGSDAWGSLHLLDTGQVLPLSNKNEFTMGRISEGQPIMPDIDL
                     SPYQAYAAGVSRLHAVIKREAGTGRIIFIDLGSANGTYINGKRLTPNVEQVLVHGDIV
                     ALGKLKLQILVKSG"
     gene            complement(14219..15700)
                     /locus_tag="PL000_02555"
     CDS             complement(14219..15700)
                     /locus_tag="PL000_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC87804.1"
                     /translation="MASSKSDHYATLGVFRDASPEEIKRAYFEAAQRLHPDKNVAAGE
                     TELFLEIQQAYEVLSNPKRKNLYDATLPPEINVNTIVKHDVTFSRPNLVKIGEPQLLY
                     ALLEISPREDKESLPAPPLNICLVLDRSTSMQGDKMDMVKATAVQLLRSLRPEDVFSV
                     VAFSDRAEVIVPAAFNVDKKKQEARIQMMQPSGATEIFNGLKAGLQEVRRTIDPSRVN
                     HIILLTDGQTYGDEQECLKLAEEAAAQNIGITGMGIGHEWNDIFLDALASKTGGSSAY
                     ISKPKDIQQLLVDKFNALISTYADEVILDFKPRDDIKIKYAFRLQPEGGSIAIETPMR
                     LGPILRDAPLHVLIEFIVEPSALDEEVVTVLNGMLKVSITARAAPVPPIRINLKRDVH
                     SNPSAEPPPTRILSALSRLTMYRMQERARTAADAGQYDEAARHLHNLATHLLSKGENS
                     LAKTALFEAENLEKMHAWSASGTKDIKYHTRALLMGGSKEKVK"
     gene            complement(15703..16620)
                     /locus_tag="PL000_02560"
     CDS             complement(15703..16620)
                     /locus_tag="PL000_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC87805.1"
                     /translation="MADFFRKLFGTKPTEEPAKPKQVDMATTAPLSDQQINSIIANQY
                     VRYETKQVIASVGQSVGKQREHNEDSLLAITSTIAGSTESIPFGLYIVADGMGGHQFG
                     EVASNAAVRIMGGNITKKFHSYLYNLPTQALQESLQEVMESSVMEAHQYVQREAPGSG
                     TTVTAALVLGQQVTIAHVGDSRAYSIYPDGRIEPITRDHSLVKRLEELGHLSKDEAAN
                     FPHRNVLIRALGQGESLEADVFTVPFPQAGYLMLCSDGLWGVISEKDIYRSIAEAPNL
                     HRACQNMVEAANLAGGPDNITVILVQMVG"
     gene            complement(16633..18429)
                     /locus_tag="PL000_02565"
     CDS             complement(16633..18429)
                     /locus_tag="PL000_02565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC87806.1"
                     /translation="MDRYNIQDVIGVGGMGSVYRARDLHFPNVVKLVAVKEMINSAPD
                     PLVRQTIVQNFEREANLLATLNHPAIPRIYDYFSLDDRSYLVLEFIHGKDLEAVIGDS
                     NGFLPEDQIISWAIQLCDVLAYIHGHKPDPIIFRDMKPSNVMINHNGDVVLVDFGIAK
                     TFQSGQKGTMIGTEGYSPPEQYRGEATPLADIYSLGATLHHAITRRDPRLEPPFSFAE
                     RPIRRINPNVSLEFEAVVNTALQYNPGDRFSSTAAMKDALLNVARKTGTLEKVAAALP
                     VSSGGVKPLWTFKCEDEIRGTPALHQGMLFIGCYDNNLYALNAADGQFQWKYATEGGI
                     VSRPTVFDNNVIFGSEDHRLHVVSIRSGKVVWTYYTEGEIYSSPRIADGHIFFGSDDQ
                     DLHAVNITSGRAKWKFATDSPIHSTPLVANEMIFFGNESGGFYSVDFRGEQKWRFQSK
                     RAVTSSPVIKGQAIYFASVDGTLYALDSKNGWPIWKFRMGKGSISSPAIADDFIFVGA
                     ADGFLYCVDTRNAKEVWRFRTENQVSGSPVIYKDSLYCGSVDGSLYCLEYRTGRLRWK
                     FETAAAITGSPIVFDDIVYFGSTDHQVYALFA"
     gene            18658..19284
                     /locus_tag="PL000_02570"
     CDS             18658..19284
                     /locus_tag="PL000_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC87807.1"
                     /translation="MKILAVSDQAIERLYTLSASGHFQDVELILGCGDMPFEYLEYLV
                     TMLNVPLFYVPGNHDPQHNPRQESVRPEGGVNLDLKTVRHKKFLIGGFGGSIRYRPDG
                     VNQYSQQEAYYRAFSMLPRLLAYRIQYGRPLDILISHSPPFGIHDDSDPAHQGLKTLN
                     WLLRITKPRYHFHGHTHLYRRNLENVETNIGLTKVINVYPYKTIEVSH"
     gene            19287..20144
                     /locus_tag="PL000_02575"
     CDS             19287..20144
                     /locus_tag="PL000_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87808.1"
                     /translation="MSDELTSRVRADFSRARFKSFINQAIAVLKGQRPTTLLSYDDVK
                     EKLHIGGPIYRGVKTIRVEQIAGSLNRYHEFDRAFLPREDQLASRWQKVDRAFYQDVN
                     LPPVVLYKVGEVYFVVDGHHRVSVAREQGQEFIEAEIRECATRVNLTPNIQPEDLEIL
                     GTKVHFLERSSLDHIRPEANIKLTIPDGFERMLEHIAVHHYFMGLDLKRDVTEEEAVA
                     HWYDTVYMPIIDVVRKSDILKNFPDKTEGDLYLWTLDHQHYLSKEKGQPLQPPHEAAQ
                     RFIRKKKKK"
     gene            20147..21082
                     /locus_tag="PL000_02580"
     CDS             20147..21082
                     /locus_tag="PL000_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HNC87809.1"
                     /translation="MTDLHPLAGVYAAAVTPLLDSPAKGSKLDSNFDLEAVPALLRHL
                     AASGCHGALLFGTTGEGPSFSPKEREVLLRSVRVYRQQLPGFKLLAGTGTPSLSETIE
                     LTKLTFDLGYDGVVVLPPYYFRKAADEGLFKWFSELITKAVPAHKYLLGYHIPSAAGI
                     GFSLDLIARLKDAFPNQFAGIKDSSHDEAFSAAVGQRFGRDLLVLNGTDSHFHHALKN
                     HAQGAITAMANLIGSDLRDIWDLYNEGGDPSEIQVRVAKQRHALEKYTPFPPTLKALL
                     HKLHSLPRWTVRPPLEDISPELEDQILQGWESLQV"
     gene            21137..21949
                     /locus_tag="PL000_02585"
     CDS             21137..21949
                     /locus_tag="PL000_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNC87810.1"
                     /translation="MNWRLLYTPPSSGAWNMAVDESILEHIHRGESLPTLRLYSWNPP
                     CLSLGHAQSFADVDVERLRANGWEVVRRVTGGRAILHTDELTYSVTGGADEPVLAGGV
                     LESYNRLAQALLFAIRELGLPVEIKEHISEPATLNLNPVCFEVPSTYEITVEGKKLIG
                     SAQARKKEGVLQHGSLPLTGDLTRICQALVFRDETARASAAQRLLARATTVESTLGVN
                     PDWETAAQAFVKGFESQLGIHFQRGELSASESERAEELIQEKYANPSWTERS"
     gene            21946..22998
                     /locus_tag="PL000_02590"
     CDS             21946..22998
                     /locus_tag="PL000_02590"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC87811.1"
                     /translation="MKNFLIYGSYGYTGSLIVEQAVKAGMKPLLAGRDENLLRTQAEK
                     FGLEYRAFSMDHSAALDAALLEVNAVLHCAGPFVLTYRQMVEACIRTRRHYVDISGEI
                     EGFEALAAMDEEAKRVGIMLLPGGGFDVVPSDCLIAHVAKKLANPTHLEIYIKSIGSG
                     VSRGTARSGIENMQRQGRIRRNGEIVSVPNVWDSKQVDFGRGPSRVVSVGWGDVSTAY
                     HSTGIPNVTAYMGFPAAMVNGMRLTRHLGFLLYNRAAKDFIKWLIGVFLKTGPSRQQN
                     ENGFSLMIAEATNGKQTVRAKLRTPEAYYLTALTAVEIMKRIASEDFKPGFQTPSKAY
                     GSDFILQFEGVTRDDL"
     gene            complement(23080..24246)
                     /gene="ftsZ"
                     /locus_tag="PL000_02595"
     CDS             complement(23080..24246)
                     /gene="ftsZ"
                     /locus_tag="PL000_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC87812.1"
                     /translation="MDTNKRNPNTEAFARIKVIGVGGAGQNAVNRMMEEGIQGVEFIS
                     ANTDAQALTLSRAPIRVRLGDKLTRGLGAGGDPEIGRKAAEESSDELYNVLKGADMVF
                     VTAGMGGGTGTGAAPVVAQVAKECGALTIGVVTRPFTFEGGKRTQSAESGVAKMKEHA
                     HTLISIPNDRLLQLADKKSSLQDAFRMADEVLHQGIQGISELITIPGLINLDFADVKA
                     IMSEGGAALMAVGRGSGDERAKIAAEQAISSQLLDITIDGARGVLFNVTGGSNMTLFE
                     VNQAAAIIRETAHPDVNMIFGAVIDPEMGDEIRCTVIATGFERTSVPRRALERPAART
                     EKPTAASASALFARSSESVSVSADYKTPGESKPASQPSISNDDLDIPTFLRNRR"
     gene            complement(24373..25617)
                     /gene="ftsA"
                     /locus_tag="PL000_02600"
     CDS             complement(24373..25617)
                     /gene="ftsA"
                     /locus_tag="PL000_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNC87813.1"
                     /translation="MDEIVVGVDVGTTKICTLVGRVEDAKTIRILGVGIEPSDGIRKG
                     IIVDLSAASQAIKRSVEKAENTSGLEITTALVSLAGAHVSSVNSRGASGIPGGIIDAM
                     DIARALEQAQAVAIPHDREIVHVIQRGITVDGQEGIRTPIGMHGYKLEVETHIITAAA
                     ATVDNLRQCVGAAGVEIQQFVLNPLASAEVVLTEQERQMGVAVCDIGGGTTDLAIYVN
                     GDVWHTMVLAVGGNHVTQDIAHGLRLPMAQAEEVKKQQGYAIRSDIASDEYFSIRPFG
                     EDNDVKINRQDLAHIIEARIEETFRLILQEVKRSGYDGLLPAGMVLTGGTSALPGIKQ
                     IASEVLGMPVRTAQPENLVGLVDKLGSPAYSTSVGLLRWATTMRDHDVIVTSGNKNRR
                     RSRGESSMNLDSIKNWMKRLLP"
     gene            complement(25620..26651)
                     /locus_tag="PL000_02605"
     CDS             complement(25620..26651)
                     /locus_tag="PL000_02605"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNC87814.1"
                     /translation="MSERQRPTRAELARQRRNQRARKELELTTKRALKPMQSVTSRVP
                     EIVVAPKLTRQSAPRRRFNIALGLPEIHLHKPQFHMPRIRPSWRLTSLVLVVLLGVVI
                     YLSLTLPYFHVPNITLLGGGRLSREEIEAVLAVNGQSIFTVQPDELEVRLRMNYPELS
                     SVDIHAYLPNHVYVTVVEREPVILWQQGGGFTWIDASGVAFRPRGMVATLVPVNGLST
                     PPAGQPATEDPLSPMPYVSKELVDSILILAPNVPAGATMFYDAANGLGWEDERGWKAY
                     FGISAHDMPLKIRVYQSLVASLTSRGTVPVFISVVYPDAPFYRMADLELDATDAASED
                     AIIVEDGQQ"
     gene            complement(26648..27760)
                     /locus_tag="PL000_02610"
     CDS             complement(26648..27760)
                     /locus_tag="PL000_02610"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNC87815.1"
                     /translation="MKKLRVAVLFGGRSGEHDVSLMSARSVLNALDAERYEVIQIGIT
                     LDGDWFTGPNALDAFENGSVDGLDRVVPPWEPSYQSLYVIRSTKLGEKLETLSGVDVF
                     FPVLHGPFGEDGTIQGLLELADVAYVGAGVAGSSVGMDKGIFKDVMRANGIPIVDSIL
                     ILRDELEKDMNAVIAKVEQMGAYPFFAKPANLGSSVGISKCNSRSDLGEGLMEAARYD
                     RRIIVERGVGNVREIEVSVLGNEYPQTSVCGEVQPSREFYSYESKYIDGTSGLIIPAI
                     LPADVSDQIRDYAARAYKAIDCAGMARADFFFDNDMKKIYLNELNTLPGFTSISMYPK
                     LWQASGLNYPQLVDRLIELAFERKAQRDHTERRRTA"
     gene            complement(27789..28718)
                     /gene="murB"
                     /locus_tag="PL000_02615"
     CDS             complement(27789..28718)
                     /gene="murB"
                     /locus_tag="PL000_02615"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNC87816.1"
                     /translation="MDMARSALHVDVLRQIFGDAVQENISLAPYTSARIGGPADVLLT
                     VKSADEFAEAMKVIWENDFPYYILGGGSNVLVSDLGLRGVVVLNRAKEVRFEGGDQPT
                     VWCEAGVIFSNLANRCASKGLAGLEWAATVPGTVGGAVYGNAGAFGGDVSMNLIWADV
                     LTKNGRERMSVEQMGYGYRTSVLKRGEIHAIVLSALLRLKNSAKEEVSVKIEQFSERR
                     KATQPPGASMGSMFKNPEGDHAGRLIEAAGLKGVRIGNAEISTLHGNFFINHGETRAK
                     DILALIRLTQKTVREKFGVQLELEVELVGDWNE"
     gene            complement(28721..28918)
                     /locus_tag="PL000_02620"
     CDS             complement(28721..28918)
                     /locus_tag="PL000_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87817.1"
                     /translation="MAHLYEVIALPPLKMALFHAVDEQLTKAALINATRKLSAIPVLG
                     SENVGKQSLEKIIEHLEKKKL"
     gene            complement(28950..30335)
                     /gene="murC"
                     /locus_tag="PL000_02625"
     CDS             complement(28950..30335)
                     /gene="murC"
                     /locus_tag="PL000_02625"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNC87818.1"
                     /translation="MKRVHFIGIGGSGLSAIARLLKESGYEVTGSDRTLSPFAVDLQN
                     NGVSIYVGHHPRNVSGADIVIRSSAIPDENVEVEAAKRAGVPVYKRSDFLGQLMSEKT
                     GIAVAGTHGKTTTTAMIAWTLTALKRDPSFIVGGTLNNLKVNARAGKGSPFVIEADEY
                     DRMFLGLKPQIAVVTNLEHDHPDCYPTFADMYSAFQGFVDLLPEDGTLIVCAEDEGAV
                     SLLSYARKKGLSVISYSVQSELTINSPQWMQARTPKPNTRGGFDFSAMTNMGTESASV
                     VNVSLQVPGEHNVRNALAALSVMATLGLPLQEAADALGEFTGTGRRFEIRGEQKGVIV
                     IDDYAHHPTEIRATLAGAKARYPDRRIWAVWQPHTYSRTQTLFFEFSRAFSDADEVLV
                     TEVYASREAKQEFSSAEVVGSMPHASARYSGSLEDTTAYLLKNLRSGDVLLVLSAGDA
                     DQISTNVLKNL"
     gene            complement(30406..30933)
                     /locus_tag="PL000_02630"
     CDS             complement(30406..30933)
                     /locus_tag="PL000_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNC87819.1"
                     /translation="MMSVVYIFWMYIILFGIVGAMRGWAKELMVIFSVITALAVNLLL
                     EKYIPLVRDLDKASSSMFWIRSLILITLVFFGYQTVNLTRFSGKAAREKLQDGMFGAV
                     LGGFNGYLVAGSILFYNHMALYPYSNIISPATDPAIAQAIETMMKYMPPRFLGEPSIY
                     FAVIIVLVFIIVVYI"
     gene            complement(30939..32048)
                     /gene="murG"
                     /locus_tag="PL000_02635"
     CDS             complement(30939..32048)
                     /gene="murG"
                     /locus_tag="PL000_02635"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNC87820.1"
                     /translation="MTNGGLTVRLLICAGGTGGGVYPALAVHNALTSQRQKVETLWVG
                     GEGGMEEELVNRAGIPFRSIPAAGVHGVGLRALPGNLAKLTRGVQASRRILREFKPDV
                     LFFTGGFVAAPMAVAGRNIPTALYVPDIEPGLALKFLSRFADVVTVTAPDSKKYFNRP
                     ERLVLTGYPLRSDLSNWSREKATQHFGLDPQMPTLLVTGGSKGARSINMAVLKHLHEL
                     LGAAQVIHITGSLDWPVIEKAAQTLPADLQSRYHAMPYSHEMGAALAAADLVLSRAGA
                     SSLGEYPFFGLPAVLVPYPYAWRYQKVNADFLVEKNAAVLLQDELLEENLPSVVKGLL
                     LNKTKLESMRAAMQALSHPDAAGSIASQLVKLAGA"
     gene            complement(31933..33186)
                     /locus_tag="PL000_02640"
     CDS             complement(31933..33186)
                     /locus_tag="PL000_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HNC87821.1"
                     /translation="MGTGTFVNEQTYKKPRFTGGFDMPLLVVVVALIVFGLAMLYSSS
                     WDFSLGAYGDAMYMFNRQLTWLGLGLFSALVLAFFDYHNWRKYVVPAMALTIGLLVVV
                     LLMNEIRFGAKRAILGGSIQPSELAKLVSILYLSVWLYAKRQYLHDISLGLIPLGVIL
                     GVVGGLIYQQPDLSAAATVLMLGGLLFFLAGGDLKQIGVLLVVATVAAWIVASISATG
                     QQRVGDFIAGIKDPLQASYHVRRSFEAIVNGGWFGVGLGQSQSKLTGLPVPPTDSVFA
                     VVVEELGWFGAVGLIVLYGALVWRGMVIARRAPDMLGTLLATGLVTWIAIEALVNMAV
                     MVGLLPFAGNALPFISAGGSNLVATMASLGILFNISRQTGEGTISDDEWRSYSAVVNL
                     RWWNGRRGLSRTRRTQRANQSAPES"
     gene            complement(33165..>34103)
                     /gene="murD"
                     /locus_tag="PL000_02645"
     CDS             complement(33165..>34103)
                     /gene="murD"
                     /locus_tag="PL000_02645"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNC87822.1"
                     /translation="DPLINYVDDMTADDLAILEISSFQLEQMTISPNIAAILNITPNH
                     LDRHGTMEAYTNAKARILEFQTEKDTAILNHDDKGSWNLRNKVKGKLMTFSLHDLDEG
                     FNGSYLHEDGLLSLRDGYAYLPLMLREKIKLPGLHNVANVLAAFTIGHAAGFKLDAML
                     EVVEEFRGVAHRLEFVRERNGVRWFNDSIATAPERAMAGIQSFDDPIVLMLGGRDKDL
                     PWDEIAKLIHKRVDHVVLFGEAAELIQKAISAVGGERSVDLRRARHLKEAVALAAEAA
                     TAGDVVLFSPGGTSYDEFKDFAERGEAFRKWVQELS"
     assembly_gap    34104..34203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<34204..34512)
                     /locus_tag="PL000_02650"
     CDS             complement(<34204..34512)
                     /locus_tag="PL000_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87823.1"
                     /translation="MTNWNSTRTLILGAARQGIALARWLSRHGSKVTLSDSRSLEQLA
                     SARESLADTDVTWAAGGHPLELLDATDVLCLSGGVPLTLPIVQEAVKRGIPLSNDTQI
                     F"
     gene            complement(34585..35589)
                     /gene="mraY"
                     /locus_tag="PL000_02655"
     CDS             complement(34585..35589)
                     /gene="mraY"
                     /locus_tag="PL000_02655"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNC87824.1"
                     /translation="MRQIALSLSLAGLAFVMTAIWGGPLLRILRHYKIGKIIRVEEPG
                     HHGVKMGTPTMGGVMFIFPVLLFSGLLNAVALIGGRSDVVGVGRSILLPMAVMAGFAV
                     LGAVDDWEGIRGKRKGDGMRARTKFMFQVILALAAAYVLKYQMDAPDMYFPGLFFEVE
                     LGWWYVPIAAFIIVAESNAVNFTDGLDGLAGLIAATAIAAFGGIALMQEQVYLARFCF
                     ILVGALFGFLWFNVHPAELFMGDTGSLSLGAVLAVVALMEGQWLMLLVIGIIPLSEML
                     SVVIQIGYFKWTKGKRFFKMAPIHLHFELLGWSETQVVQRFWLIGLMAALIGIGLSQV
                     "
     gene            complement(35586..37001)
                     /gene="murF"
                     /locus_tag="PL000_02660"
     CDS             complement(35586..37001)
                     /gene="murF"
                     /locus_tag="PL000_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC87825.1"
                     /translation="MLTLADALEAITSVRIANANAVITEAAIDSRQVIPASLFVAIPG
                     EKMDGHDFIADAFKRGASFALTQKEMPESFRTLDLRNGLTAESPLDLSTPLCLRVDNT
                     ISALQQIARFWRRKLDLRVVGVTGSVGKSTTKETIAEVLGTRYRTLRNPGNLNNEIGL
                     PLTILKLGSGYERAVLEMGFYVPGEIQFLCDIAQPQVGVVTNVGTVHAERAGSQEAIA
                     RGKSELIQALPADGVAILNFDDPWVRKMEEKSKARVFFYGLSPEADLWADGIEGLGLD
                     GIRFRLHYKGETVHSHIPMIGRHSVHTALRATAVGLNDGMTWQEIFEGLLQGHAQLRL
                     VAVRSNTGALILDDTYNASPESMLAALNLLDEMDGRKIAVLGDMLELGPYEKQGHEMV
                     GMRAAQVARVMLTLGPRAHMIADAARRTGMKSANILEFENSEPIIDWLNKNLTSNDAV
                     LIKGSHGLRMDRITAALETQS"
     gene            complement(37003..38751)
                     /locus_tag="PL000_02665"
     CDS             complement(37003..38751)
                     /locus_tag="PL000_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC87826.1"
                     /translation="MRQQYARRAQVLTFVMGLIALAIVVQMTRIQNSAEAAVFRQQAS
                     NYAYELRTFYPDRGEIYDRNGHLLAGNKSVYELGVDLSTVKDPHAIAFALSSELGESY
                     DRIYDMIINPPDGLSYVVVADYVESAQAIRLQELKKALQEQAPEGTLGGLTGLQFKSH
                     PQRSYPEFSLGSNIIGFVNREGKGYFGIEEKYDTLLAGNPVQVLVPTDPNKAFEIPRV
                     PNGTTLVLTINRDLQAAAEEILDRALIEHGAQGGTIAIMDPRNGELLAMAVTPRMDLN
                     EFWNYGAIYDNASDFNPAISMPYEPGSVVKILTMAAALDTGTVTPETTYLDTGSILVG
                     GATIQNWDQEPWGVQDMTGCLQHSLNVCMATLSTQMGAGNFYSYMDNFGLGQLTGVDL
                     AGEAPGRLKIPGDADWYPVDLGTNSFGQGLTATPIQMMMAASAIANHGRVVTPHALYA
                     MMRDGRQYNVPAQFAGTPISQQTADILSGMLAVSLESEGSLALVPGYRVAGKTGTAQI
                     PVNGFYDSTQTNASFIGWGPIDDPRFMIYVWLERPSSSIWGSETAAPVFAEMAQKTVL
                     LMDIPPDSIRQQLVVK"
     gene            complement(38748..39221)
                     /locus_tag="PL000_02670"
     CDS             complement(38748..39221)
                     /locus_tag="PL000_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87827.1"
                     /translation="MNIPNVNHYIHAYKIAPWRVQRQWIGNALLMVVALAMISTLYLD
                     VTSQAAIAGREIQDLTSSIIVSQQVSGDLQTHLASLTSASVMRQRALELGFRPMEAEE
                     VEYLVVPGYVTLEPDILSSAPLPQLSALTFPPEYNQSLLDWMDNRITSSASRGTQ"
     gene            complement(39239..40153)
                     /gene="rsmH"
                     /locus_tag="PL000_02675"
     CDS             complement(39239..40153)
                     /gene="rsmH"
                     /locus_tag="PL000_02675"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNC87828.1"
                     /translation="MQHKPVLYQEIIHALQPRNGGRYVDGTLGAGGHARGILEACTPD
                     GLLLGLDVDPQALALARETLAPYEGRIHLVQASHITLSQQLAALKWDSIDGIVLDLGA
                     SSMQFDNAERGFSFMQDGPLDMRFGINAVMSAEEIVNTFDERELADIIFRYGEDRDSR
                     KIAKAIVMNRPLRTTRELVAVIEKASPRHGDRVHPATQTFQALRIAVNDELAAVEKTL
                     PQAVAALRSGGRCAVISFHSLEDRIVKEYFREQSKDIVNPPYERIYEEERKAVVKIIN
                     KKPIVASDEEIKDNPRSRSAKLRVVEKL"
     gene            complement(40171..40617)
                     /gene="mraZ"
                     /locus_tag="PL000_02680"
     CDS             complement(40171..40617)
                     /gene="mraZ"
                     /locus_tag="PL000_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNC87829.1"
                     /translation="MFLGHFQHNLDDKGRLMIPARFRELLAGGAFITQGFDKCLMVMT
                     EDYFKQVYERIEAMNLADPTARLLRRLILSNAYPIEVDKVGRILVPQNLRAFLGIESG
                     ELVVAGQGEYFEVWTPAHWGEQMAQLQDTEANNARFSTLDLSKPAN"
     gene            40775..41677
                     /locus_tag="PL000_02685"
     CDS             40775..41677
                     /locus_tag="PL000_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC87830.1"
                     /translation="MQKPPTFSLLEASNWRDYELLDSGDGQKLERFGKYIFSRPESQA
                     MWSRALPSSEWNNAHAVFQPTAEESGGHWIEKKKLPEKWEMKYPLPHVQDLTFSVMTT
                     PGRHLGVFPEVASHWDFMADSVRKAGRPAKVLNLFGYTGLASLAAAAAGAQVTHVDAS
                     KKSVGWARENQALSGLNEKPIRWIVEDAVKYVQREEKRGVKYDGIILDPPKFGRGPKG
                     EVWEVYKSLPNLLEMCRACLSDSPLFVIVTVYAVRASAIHVAQAMDEMMKKYNGNIDS
                     GELVTREKSANRLLSQAVYARWSK"
     gene            complement(41718..42641)
                     /locus_tag="PL000_02690"
     CDS             complement(41718..42641)
                     /locus_tag="PL000_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87831.1"
                     /translation="MSISLVLEVAVGLIVIYYILGSVVSLITQWINEFFESRGRALEQ
                     YLIKLVGDKKIGDLKNLPQLQSLRPIRFKGPLSVFGSVTEPKMIEKIPVATLVDAYFD
                     LAGLTAKKEMELLELAELVDRLPDSEGKQAFITWINQGVTSIAELRVRATSYFTGILE
                     QAAATFRARARSFVIILSIGITILFGTDTIQLARNLWTNAELRAIAAAQANAVVAREG
                     ASADLSDLIDDLSQYSIKIGWWQTQQFPEGNNVGDWALFIFLKVLGLGVTAAAVSQGS
                     SFWYDLLKKLTSPTSSSSGSKSSPSGGSSSG"
     gene            complement(42668..44233)
                     /locus_tag="PL000_02695"
     CDS             complement(42668..44233)
                     /locus_tag="PL000_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87832.1"
                     /translation="MTELEILEKYEPVLRFAKSERFFPMAVEPYLEKCHIFPGGPQGV
                     LEIVMHIHEPMITRIGKLQSEQFFLRFINKPLNDADAWVWWGLLSAAGMVGGWLTANF
                     AGVEFMFVASLLGALVLFMLASPIRLRIIPAVLAAGAFITAEVTPIWFFLHPSRVMSV
                     AVEYLVLLPIYLVILFYLSMRTLKFILDRVVPEGPGVVMDMFSQATEKIAREAYFEYA
                     AMLAMDAQPVYYGRVLHEKDEDGCAWTILQYHFFYAFNDWRLAANGMNHHEGDWEMVA
                     VYLKNNEPYAVLFSQHGAGNIEKWKTVRKAVDANGHETTHPVVYVALGSHANYSKPVV
                     IRSPWMYKPGKLQQVLFWLDGFIHYLFLLLNPNQRAREIALREFWKQGGKFLTEEMFA
                     RLRDETDHYIVTLPMEIASGDGHRIGLSGQGSHEGFIRSSRYIKRVMSDRQTTQPQVA
                     EWRRVLLNPEPDWVQYKGLWGVKSMLGEESGPPGPKWDRPGKKQVGVQERKRWGRPLE
                     WLAELEKTVPDRK"
     gene            44260..45429
                     /locus_tag="PL000_02700"
     CDS             44260..45429
                     /locus_tag="PL000_02700"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNC87833.1"
                     /translation="MNTKTLEQSFLTHFNAKPAFIVRAPGRVNLIGEHTDYNDGFVLP
                     MAIDRAVWIALSPRMDSQVHIHSLDMETALPNDPQAVSTFDLNSLTKGKGWAEYIKGV
                     ADQLQKAGYQLHGFDAIITGDVPRGAGLSSSAAVELAAARAFAVTAGFEWNAAQMAAL
                     AQKAENEWVGVNCGIMDQMASAASQAGHALFLDCRTLEYQLPPLPKGIAVVILDTSTR
                     RGLVDSAYNERRNQCEEAARWFGVKALRDVSVAEFNEKTKDASELNVNVLKRARHIVT
                     ENERVLEAVEAMKLGNIQRLGELFNASHSSLRDDFEVTNEALNQIVACAQEQPHCFGA
                     RMTGAGFGGCAVALVREENAAAFTQAVSAAYKQRSGLEASIYVCKASDGAGVIQG"
     gene            complement(45474..46493)
                     /locus_tag="PL000_02705"
     CDS             complement(45474..46493)
                     /locus_tag="PL000_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNC87834.1"
                     /translation="MYICVLTSVPDENDIPYDPSPYMNGYRWKQHLVEPKNVEKQIRD
                     LMDEGVDVFINLCDGTPDDALSGIALVNALEKHNAAFTGADSKFFDPTRDEMKNAARR
                     VSVPTPNWLFVNRVEDAEKVAKRLKFPMLVKPPHGYASVGIRRNSRVETIEQLREQLK
                     LEIDEFGRALIEEFIEGREFTCLIAENPDDPKKPLTFTPVEFIFPNGESFKHYDMKWI
                     DYEKMSVAVVDEPKIQKTLREQTMRVFKELGGNGYARCDYRMDANGDIYMLEINPNCG
                     IFYPPHEPGSADFSLLNDPINHTKFMKLIVRNAQMRQTRIAAEKIIKRQTRKRPIAVE
                     QMAYS"
     gene            complement(46836..48221)
                     /locus_tag="PL000_02710"
     CDS             complement(46836..48221)
                     /locus_tag="PL000_02710"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87835.1"
                     /translation="MTTITVIGAGSASFGENTLSALLRSKKLNGSTLRLVDRNAQSLD
                     IVHRLAARLNKEWRSGFNITAHTNHKDALDGTDFVVSAIEVGPREELWKADFDIPIKY
                     GVRQPYAENGGPGGFAHTARNAMPVLNIVRDMEQACPDAWFINFTNPMVRICDLVNRH
                     SKIKAVGLCHQIFIGYCFVGMVLAEELGIEVPKGITGMQADVVEHDLRDHVVHQTLPL
                     VDLRAAGTNHFTWILSLHDKRTGEDLYPLFRERFKAFDPSFEPLTREVFDAFDLFPVP
                     GDTHLCEYLPWLSDPVTKPWEKFNIRLYDWDVRANLRDVSLDRLNDMANGTVAIEGLL
                     NTDSEGALEMIENIAYAGNHYHLAANLPNVGQITNLPLGATVETPVHVDGAGIHPVHV
                     GALPEAVAELCRRELMVAQLSVDAAVEGDYQKALQCLLLDPVITDMHVAKRILDDYLL
                     TYKEHLPQFHG"
     gene            complement(48380..49180)
                     /locus_tag="PL000_02715"
     CDS             complement(48380..49180)
                     /locus_tag="PL000_02715"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNC87836.1"
                     /translation="MKKMIWMTFLAATLILAACGTPATPTPPPIQTEEPASPQNDFVS
                     SDAVVASAEVIPAQDSQISFVISAPVKEVLVKEGDTVSAGQTLMTLASPDLELAVTSA
                     ELELKAAELEFTYWIPARHDRPPERRQQAEAELEQARKKLETAKANLAQTSLLAPFDA
                     TVIDVNIQAGELAQVGKAVITLGDIAHMQIATTDLSERDIPNVKIGQAVNVYIEALDI
                     TVTGKVLHISPVSETVGGDVVYPVTISLDEQPEGMFWGMSAEVEIMIK"
     gene            complement(49191..49973)
                     /locus_tag="PL000_02720"
     CDS             complement(49191..49973)
                     /locus_tag="PL000_02720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC87837.1"
                     /translation="MRKTLLIVFVILATALTACGGQATPEAVPTISLDAPSQSSGGSA
                     TASAVVVPVTKVELSFPLSGAVKTVEVAEGDSVTAGQPLVTLDTAILEARVAEAEANV
                     VTVTTQLTYLKRTGTSQERLDAAQANIDRAMAAVEIAKAQLSQATLVAPFDGTIASVE
                     ISPAEFASPGQIIIVMGDLTHFQIETTDFSEKDIPSVSIGQAAQVFIEALDQEFTGKV
                     VDIARSSETVGGDVVFKVTIQLDEQPDGLRWGMSADVKIEEK"
     gene            complement(50076..50762)
                     /locus_tag="PL000_02725"
     CDS             complement(50076..50762)
                     /locus_tag="PL000_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87838.1"
                     /translation="MEVVKIENVTRVYKIGKVETQALRGVNLSIGSGEFTALVGPSGS
                     GKTTLLQMIGCLDQPSSGHVLISGKDVTTLNRNQRADMRRGTIGFIFQFFALIPTLTA
                     YENVEMPLLLNGHSPKERHERVMDLLKAVDLTERANNRPDQLSGGQQQRVAIARALAP
                     KPTLILADEPTANLDTANGKQVMDIMKKLNKETGVTFVFATHDPRVISYADRIVTLND
                     GLIEKDSSSS"
     gene            complement(50771..51991)
                     /locus_tag="PL000_02730"
     CDS             complement(50771..51991)
                     /locus_tag="PL000_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87839.1"
                     /translation="MMQILKMAFRDLGRNRRRSFFSALAVGGGLALLILMSSVVAGEM
                     GSAIESAINLQTGHIQIRAATYDENKSSLKWEDLVANPDEIAGKIAALDQVETATPRL
                     YASGFLSSGTQSAGAKITGIDPLSPASDPYREGVISGEYLNPEDRDVLLIGKPMADKL
                     NLKVGDSVSLSLNTADGNVQDQTFTVKGIYSTHTYAFDSATVFLPLAKAQAMTRTEDH
                     ASTIFVLLKDTEMTDAVVPALSVSSNLEIKTWKDLNALFVEYETFAQSYIAIFYMIIL
                     AISASVIINTLIMSVYERTREIGVLSAIGMRGGRIMMLFLAESSMLAVGGVVMGLIIG
                     VLATLYFNVNGFYIGDMGLSGMAIADTIYAKLTVENIVNLTIMTFVVTLLAGLYPAIM
                     ASRMQPVEALRAEK"
     gene            complement(52030..53256)
                     /locus_tag="PL000_02735"
     CDS             complement(52030..53256)
                     /locus_tag="PL000_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNC87840.1"
                     /translation="MQLYLRLAWRNIWRHKRRTVIIVLAMSLTLALMMWYDGLMNGFT
                     DAIYGNAVKILGGNVQVHAEGYRAEANSNPLFPLTDPQAVIDAAEANPLTLAATQRIS
                     TGGLVTSREGAFSVGIIGIEPEKELTVNIIGQNVKEGRNLTSADLDNVLIGKGLADAM
                     GVKVGDRITMVGRSQHEQMRQRTMTVVGIFDLGLADIEKQSVYISLGEAQALYEVTGS
                     TEVAIFLEKLGQESDVIEALQPSLPGYEIESFQANYPDLASAINTKGGVMDVFSVIII
                     AIAGVGILNLLLMAVYERTREIGVLGAMGLKPSQISMLFVLEGIMIGLVGVAAGIILG
                     LAINGYLMKVGLDFGNMTQAASYMALIQSRAYPTWGIEKLPLRVSMILIISALAALIP
                     AAEAGRREPAEALHFV"
     gene            complement(53304..53993)
                     /locus_tag="PL000_02740"
     CDS             complement(53304..53993)
                     /locus_tag="PL000_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC87841.1"
                     /translation="MPRVVKEEDYVARRNEILDVARQLVYTKGYEQMSIQNILDALNI
                     SKGAFYHYFDSKQSLLDGLIERMLDEAEMVLRPIVESKDLSAIEKMRRYFDTAGRWKV
                     AQKAFMLDLFRVWRADANAIMRQKQEAASIERIGPMLTTIIQQGVDEGVFSTRYPEQF
                     GNIFVGLSHGFEDKFVELLLTDHPPPDAVKRLEALIGAYSDSVERILGAPPGSLPLGD
                     IETMKEWLPKP"
     assembly_gap    54152..54251
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<54252..55037)
                     /locus_tag="PL000_02745"
     CDS             complement(<54252..55037)
                     /locus_tag="PL000_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87842.1"
                     /translation="MENKKTPFWTRDSSILLGGFFVTIFLIVYIWWPLAEEVLSYIDW
                     NGPWWLYMDWLLLGVFLFMSITIVARANLKTDVLIVFVGICGGLAIESWGTQTNLWHY
                     YTAERPPLWIIPAWPIASLSIDRITRLLSWMIERFQIRFKRSIHHSAFSILYWIIFAS
                     FLTLMLVFVSPTFDKSYTWLATILCILLILTPTDHRLALLTFIAGAGLGYFLELWGTT
                     RECWTYYTFQKPPLFAVLAHGMAAVAFWRAGLMVKMVMGKMGLV"
     gene            55193..55708
                     /locus_tag="PL000_02750"
     CDS             55193..55708
                     /locus_tag="PL000_02750"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HNC87843.1"
                     /translation="MSFFSTILQKLGLKKEEPAATPPAAAPAQTTSTPAPAKPPVGYG
                     PTTTASTAAAPKPAGAGTLSDRRVQDDMAVNHEPMKPAVKAISEVDVVKHLESLGAGS
                     GLDWKVSIVDLLKLLDIDSSREARNELAKELGCPEDLMHDSAKMNVWLHKTVLKKIAE
                     NGGNIPASMLD"
     assembly_gap    55791..55890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<55891..56502)
                     /locus_tag="PL000_02755"
     CDS             complement(<55891..56502)
                     /locus_tag="PL000_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNC87844.1"
                     /translation="MKAVCLALPFLAKWKARVANPRLQITQAYNLVMTFIPQPPQIPL
                     LLKLGIYISEKITGRIMLPPRILAWYPKAAIGSGIMEALVAHEDGRMNQRMLKLIRMA
                     ASYAVACPFCVDMNSYEYRKHGITDEEAESLRGDYESVASFSERERLAIAYTRLISAT
                     PLKFHPEMVDKVKAAFSEREFVILASTAAQVNFWARLIQALGIP"
     gene            56569..57576
                     /locus_tag="PL000_02760"
     CDS             56569..57576
                     /locus_tag="PL000_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC87845.1"
                     /translation="MASLKKSPTIYDVAEVSGVSISTISRVLNSPDKVNPKTRQQVMD
                     AIDKLGFVPKAEARARAMRNTNRIGVLTPFFTAPSFVQRLRGIAGALSKENYELVVYT
                     VESAAQLENYFASIPLTSNLDGLIIMSLPISEANARRLTQYDIPTVFIEYPHPTLSSV
                     EIDDVAGGRMAAEYLISKGHRRIAFLGDTDLPEYAIHPVSLRLSGFRQTLQNEGIEIP
                     DEFVRLAPYTQEQTLRVANELLNLPNPPTAIFAATDFQALGVLKAARQMKVQVPEQLA
                     IVGFDDLDLADYADLTTIRQHLDESGRLAVEILLSRISDPTRPAQHIHLPLTIIERQT
                     A"
     gene            57586..59679
                     /locus_tag="PL000_02765"
     CDS             57586..59679
                     /locus_tag="PL000_02765"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNC87846.1"
                     /translation="MPIHTSTNYWVLETRHTAYSFGLNNAKLLTHCYWGTRLPFIQDY
                     PAPFNPVHWASTDGFDHFVPEEYPGYAGTKYTDPCLKITFADEVRDVVLEYQDFEISA
                     SPIPTLALRLKDSFYPLQLTLHYRIHEEYDLIERWVTFENLGDQPMLIERAFSAKWTL
                     PPGDGYSLTHMYGRHVGEFNLQREELTPGLKVIESRRIIPSHRANPWFAVDRNASEDH
                     GEVWFGTLAWSGNFKTTAEMTEFASTRISIGLNDWDFAWKLMPAQSFSTPASISGYTQ
                     NGFGGASRLFHDYVRNEIIPHGKVPHKILYNSWEATLFNVDESSQTALAEIAAQMGIE
                     LFVMDDGWFHNRNWDNAGLGDWFPDKVKFPNGLSGLIKKVNDLGMEFGLWVEPEMVNP
                     DSDLYRTHPEWVIHFPTRARTESRNQLILNMAREDVQNYIITTLDTLLRENNIAFIKW
                     DMNRGVSEPGWPQAPGDQRELWVRYVQGLYHVWGTLRQRHPNIIWQSCASGGGRADFG
                     ILQFADQVWVSDNTEATSRLNIQEGYSQFLPANTMEAWVTDWGREIVPLEFRFHVSMC
                     GSLGVGGNLLEWDQAHRESAAGYIRLYKEIRHIIQFGDQFRLISPQNNDYSAVQYLSK
                     DKTEGVLFTFRTHIPDPFNIPMIHLRGLEPEALYSIEGFEQPRSGQAWMQAGLKVELK
                     NLQSKILRIQRLTPN"
BASE COUNT        14851 a        16146 c        14801 g        13950 t
ORIGIN      
        1 agagatcttt tcagcaatca ccttcctttt ccttaattcg gtcttcacac acgtttcact
       61 gaaaagccat attcttcaag ccccgtgcct gtttatgggc aggaaagcca atccctactg
      121 caaaaacgat aataaataga aagaatacat ggcagcgatt gttgtgtgac atttttcctc
      181 cctgaatttt tataaacaaa tatctgcagg tgggatttgt ggcacaggtg taggatatac
      241 cattcctcct ggtggttggg taacgtgata ttttctactg ggcactaccc ttactgtgtc
      301 tgcactggta ataatataaa gtatttcgga gtccccatag cgactggttg gggcatattg
      361 tgagtaagta ctaataaaga aaccccattc accttcataa aggatgcaca attcgagttc
      421 gtgggcttga ggctggtttg caattagcga gcctttccac tctaaatcgc cactcatcaa
      481 cttcacccca tcgggcaatg tgatgttgat ggtgacatct ttctcgtcat taatggacat
      541 aatctcgacg cgtagggtgg cggtttgccc aatgacagga tcgggatgca cggaaatatc
      601 cgttacctct agcggtttac tttcgcaggc agttagtgtt gccagaatca gcccaatgac
      661 aagcagggtt tccaatgtgc cttttgtgtt gcgagttttt ttcatgatgt tactccctta
      721 tttttttgat tttccgcatg ctcaagccgt catcctattc gggcactttc ctcggcggca
      781 gaaactattt tgagattcca gaacattccg aagagacggc gatggggcgc ggcgtggggc
      841 gattggcgta ttcttcctga ctgaatgtat attcactgct tgggataagc ttgcccgaat
      901 ccaatgtgct ttccaattgg attatttcaa agtcataccc cccctcctgg tcggatgaag
      961 gtaaagcggt ataaagtcgt acgggccagg agccttcttc cattacacat attttcactt
     1021 gaaatatttt gggttgattg gcagtaagtg tgccttgcca ggcaggctcc ccgcttacga
     1081 ggtgaatttt attgccagta tcttccaagg tgtcaattgt aaattttacg ttggccttat
     1141 catctacaga cacgatctca acctctaaag ttacaacctc ccccacaatt ggctcaggaa
     1201 agactttaac agaagtaata ttgagtggat atggataccc tccgcttgaa ctgcaagcgc
     1261 tcactgtcaa cccaatgaat agaaaaagac ttgccatcct tttttgtgta ttgatttttt
     1321 tcatgatgtt gctcccttat tttttgattt caagaagttc gtaatgactc atcgaacttc
     1381 ctttttgctt tgtgtgttgt ggggtttgaa aattgattta cccgccttga ttttttgccc
     1441 aaacagatta gaacaaaaaa aatacaacta tcaagctttt tgcaacaatg acgagtttgt
     1501 tttttttgtt gaattacttt ttttggaaat gttcttttaa agtatcaccg ttatttttgt
     1561 aggggcactt ttttattccc ccagaatctt ttcaaattac aaaaaaggga cacagcggcg
     1621 ctgtgtccct acgggttaga tcaatttttc gagaagcgca tccagttctt cgcctgagta
     1681 atagtagatg gtgaccgtgc cgcctttttt actgtgcttc aaggcgactt tggtgccgag
     1741 gctgttttgc aatcgctttt ccacatccgc cacatctgca ctgcggttgg gcttcttcgc
     1801 tttgatcggt ttctgccccg cgagttttct cacgtactct tccgtttggc ggacactgaa
     1861 agagaggttg atgatcgtct gcaaagcgga agcctgcgcc ttctgcgtgg acaaggccag
     1921 caatgcacgg gcgtggcctt cggtcacggt cccatctacg agggcctgtt tgaccacgtc
     1981 cgcgaggccg agcaggcgca gggtattggt cacggctacg cggcttttgc caacgcgttt
     2041 cgcaacggct tcatgcgaaa gcccgaattc ttcaacgagt tgacggtagg cttcggcttc
     2101 ttcaatcgaa ttcaagtcgg ctcgctggac gttttcgatc aacgcaagct caagcaattc
     2161 ctgattgttc gcctggcgcg tgatgacagg gacagtccgc aggcctgctt tttgagaagc
     2221 ctgccagcgg cgttcacccg cgatgatgag gtaggtccca tccgctttgg gggtgacgat
     2281 gatcggctgg atcacaccat gttcacggat ggacgcagcc agctctgcca attcatcttc
     2341 cttgaaatgc acacgcggct ggcgcggatt cggcttgatg gcttccacag ccacttgctg
     2401 aacaccaccc gtgcctcccc cgcttgatga agcgggcggg gttttcccac cagcaggaat
     2461 gagcgcatcc aaacctttgc caagtccagt tttttgagcc atagttttta ccttttcaac
     2521 caccaagggt cacaaagtat cacaaaggtt taagaaggtt tccttcgtga tactttgtgg
     2581 ttagttttta tcacccttca acaattcctt cgccagcgct tcatatgctg ccgcgccgac
     2641 agaagtgggc gcataagccg aaataggcag gccatacgaa ggcgcttccg caaggcggat
     2701 actgcgcgga atcacactct tgaacacttg atcggggaaa tgccgattca cctcagaaac
     2761 cacatccgtt gaaaggtttg tgcggttatc gaacattgtc agcaccacgc cgcgcacatt
     2821 caactcaggg aaaagcaaag accgcacacg gtttatggtt tgggtcaact gccccagccc
     2881 ttccagcgcg aggtactcac actgcacagg cacgatcaca ccgttgatgg cagccatcaa
     2941 cccatttacg gtcaacagcc ccaacgaagg cggacaatcg atcaacacat aatcatattg
     3001 actggcaacc tgctccaggg ccctgcgcaa acgcacttcc cgggcaagtt catccaccaa
     3061 ttccacttcc gcacccgcga gcgacggcga agaaggcagc agggataatt tgagcttctc
     3121 attcagcaaa acataggaag caatgctcgt atcgcccaac agggcttcgt aggtaccgcc
     3181 ctgaacgctc aacttatcca cgccaaggca ggatgtggca ttggcctgcg ggtccagatc
     3241 caccaccagc acacgctggc ccaactgcgc cagatacgca ccgaggttga tcgtggtggt
     3301 ggtcttcccc acaccgcctt tttgattcac aagcgtataa atacgcgtca ctggtatacc
     3361 tccatcaaac attcttcgat ctcatctttg gtcgggatcc cgttcaaccc gacccgtgtc
     3421 accgagcggg ccgcgagttg agtggcaaaa cgagccgcct cccacgggtc acgcgtggca
     3481 tataaacgga taaaaaatgc tgcagcaaaa atatcgcccg cgcccgtcgc atccacttca
     3541 tgcatcacag acggacgaaa acggcggcgg tcgccattcc aatgcagcac agaacccaac
     3601 tccccttccg tcatgcagag gatgcgggtc tggtgcgcca tttcttcgac aagctcaaga
     3661 tcgcgattga tatcttcgac gctcaaaacc acggctcctg cataacgaag ggcctgatcc
     3721 ctgttttccc aggcagaggc cgtcacttga ccattttcat cccattggcg catccagcct
     3781 tgcggcgtga ttcccaacaa tgagccagcc agtttctcag gcaaagatgg gtccaattcg
     3841 cgggcgatgg gcgcgaggtg tatgatcggc gcactccgcc agacctgcgg aatatgttcc
     3901 aatgtgatcg gcgcagcctg atgatgcaat acctgcctgc gtccgtgatc ggttttgata
     3961 ttttcaaaag tggtgctgtg ttccgaggga atgttgacaa tggtgatgcc atccagcgcc
     4021 tgcatgggcg caccctcccc caccgcggtg acaattccca cccgcatccc caacgcacgt
     4081 gcggtcaacg acgagtagga aaccgtgccg ccaagctgca tccccgttgg ggtgatatcc
     4141 acagcggcat gacctacaac gagataatcc acaggttgaa gtgatacaag ttcgagcatg
     4201 gacgaattat accgtaggga aatgtaagga cacggcgctg ccgtgtcctg ttttggtgaa
     4261 taaaaaaggg acacagcggc gctgtgtccc tacagacggt tattcttttg gcaggatgac
     4321 cactttgcgg tgcggttctt cgccgacgct ttcagtcgtc actgcgggat gcccgcgcaa
     4381 ctcgatgtgg atggcgcggc gttcgctggc cgtcatgggt tcgagtgtgg ctttgcgccc
     4441 ggtcttgatg acctgatcca ccatgcgcaa ggcgatctga cggacctgct tttcgcggcg
     4501 gctgcggtac ccttccacat ccacgaccag ttgcacccac ttctgggttt ccttgccaac
     4561 gatcaaggat gcgacatatt gaaaggcgtt cagcgtttct gcctggcggc cgatcagggc
     4621 gctcaagtca tctccgcgta catccacttg aatgacacgg cggtcatcgg tgctggattc
     4681 atcgtaatga gccgagacct gtgctttcat gccaaggtga tgaatcaatt tcgaaatgac
     4741 cgactcggtg gtatcgagca gggcgtcact accttcggag agggggcgct gttttactgg
     4801 ggcaggcgct tccggtgcgg gtttcttttc tgtgcggggt tttggttcgc gctgggcctt
     4861 cggcgctgat tctgcttttt gagcaggagc aggcttgccc ttgggacgat ccgcaggttt
     4921 ttgtctggca ggtgcagggg tgtgggtttc ttcaacaggc ggcgggttca cggtcaggcg
     4981 tactcgtacc tgccttccgc cgagtccaaa caggcccttg ctgcctgaat cgagcacttc
     5041 cacagaaact gcatctgccg tcaggccgag ttgggccagg ccctgtgcaa gggcttcttc
     5101 aacggttgga gcgatgattt caagcgtggt tccgctcata gtgcctccct atcttttact
     5161 tgttcttgct gccgggcagc aggttgctcc agttggcgcg acccgtggct gcatattgaa
     5221 tgataccgag taggttgctg gtgatgaaat agatagcgat gcctgaggca aaattcagtg
     5281 cgaaccagcc aagcaggagc ggcatgtaga tgctcatcat gctcgacatt tgcgccgatt
     5341 ggtcatttgg gttgctggag gtcggcatgg tcaacttcga ttgcacgtag gtggtgaggg
     5401 ccacgatcag tgcaagaagc ggaaacccgt tgggcaggaa ggatgtatga atgatgcttt
     5461 ggaagatacc ctcagggcgg cccaggtcca tccacaggaa tgtgctgttg agcgggatga
     5521 tgttctcaac gttttgaaag gggtagatgg tgcgcgccag tttgagcatg tcaaaggggg
     5581 tgatggaaag cgcgcgggta atgctttgat acaggccgat gatgatcggg aattggatca
     5641 acgtgggcag acaggaagca aaaggattga tgcccttttc cttgtaaata cgcatttgct
     5701 cctgcgcgag cttttcacgg tctttggcgt atttcttttg aatctcctgc cattccttat
     5761 cgttgttcag ttcctgcatg gcctgtgcgc cttttacctg agaggcattc aagggccagg
     5821 tcactgcttt gatgagaatg gtgaacaaaa tgatcgcaag accaaacatg tgcggaccat
     5881 gaccaagtac gtcatagatc cacaataaca ggttggtcat ggggttgata ataatagtat
     5941 cccacatggg tcggtttcct tatttttccg gtgtgaacgg ccagacttgt ttgccatcca
     6001 catcataagc aacgagggtg gtttcggcct ggtacggtgc aacgaggatg gtttcgcctg
     6061 aaagcacggg cgcggtgtag agtttgccgc ccacgggttt ttcccagacg gtgtttccat
     6121 ccaagtccaa tgcgatgagg ttacccgctt cggtcgcaac gtagatctgg ttgccagaaa
     6181 caagcaggcc agccacgacg gcatcgggca gggcaatttc ccagttttgg cttccctcgg
     6241 ttgtgttgaa tgaaaagacc ttgccagtga gatcagcgta gtacaacgtc tcacctgcaa
     6301 ggacagggga accccagatc caattttcag ttttagcaac gacctcgtga ttgccgttgg
     6361 attgaatgaa ttcaacagtg gatgcgaacg aacccacata cacaccattt tcagccaccg
     6421 ccggggtgcc gggaacggct ccgcccaggt caatggtatc tacagtgctg ccgtcagatg
     6481 ggtcaatgat gtgcagatgg tgatcgagtg aagcgatata aatgaacttg ccatctgtta
     6541 caggggcaga ccacaatgcg ccaccgatct gaatggggtc ggcggcttgt ttgccctcca
     6601 gatccagcac atagacatag ccatcagtat ttggggcata gatggtgtca ttcgcgatta
     6661 atggagaggc aagccaggct ccctttgctc cagtgaatgg ctcaacccaa ttctcttttc
     6721 catttgcagg gttcaggctg taaaaggggt gcgaggatcc cgcgcttccg atcaacaact
     6781 gaccatcagc cgtgaggacg gggttggcat aaaacatcaa tttggaatcg gcttcctgcg
     6841 ggaagcgcca ggcttcgtcg ccattgtcca tattcaccgc ataaatgtgt gtgccgctgg
     6901 ccacataggc atgttccgca tctgcagcaa ggccatgcca attgttggat actgtgcggt
     6961 tgccgctaca ggcactgagt atcaaagaac ccagtaccag caatgagatc aaaaccaatc
     7021 gttttgtatt caagatattt acttctccta aattaagtgc cacagccggg gtttcaggga
     7081 acaggatcgt acccgcccgg gttgaacggg ttgcaacgca atacgcgcca aacagccatc
     7141 aacgaacctt ttaacgcgcc atgtttataa acggcctgat agccataatg agagcaggat
     7201 ggataaaagc ggcaggtgtt tgcgggcaac cccggggaaa ttgccatttg atacaaacga
     7261 atcaaggcca gcacagcaat gcgtggccag tttgccagat tacgcggcat gtcacgcaga
     7321 cgcggctcat gggagtaatc gtgtaaatgg aattcccgtt caggttcagg attcatccgt
     7381 aggaatgatt tttgcgcgtt ggaggagggc cagcagggcg gagcgggtat cttctaaagt
     7441 agcagataca agcgaaggtc gggcaatcaa tacaaggtcc cagccggaag cgagggaggt
     7501 tagcaggggt cgcatggcct cgcgcagtag tcgcttggcc cgattgcggt ggacggcagt
     7561 accggtcgtt ttccctgccg ctacacccac acgcacatgg cgggcaggtg actcagtcgt
     7621 ctgtgctacc aacacaacaa gcgggtgggc ataggacttg ccagttcgcc gcacccgctt
     7681 gaaatcttca gatcgggaca ggcgaaattt gctctgcacc cgtgcctcaa tgtgaatcta
     7741 tgcgccctaa aaaacgcaaa tgcctaccaa cgagtcttct ttacatgctc attggctttg
     7801 acggtgagtt tgtagcggcc cttaaggcgg cggcgcttca acactgcacg tccgtcagcg
     7861 gttgccatgc gggcgcggaa gccatgcaca cgcacgcggc ggcggatctt gggttggtat
     7921 gttctcttag tcattcaaaa gtcttccttg gttaatgatg attgcaaatc ccacagggga
     7981 gttgtctgta agttagtggc gcggaattat acaccaatgc ttccgattcg gtcaatttca
     8041 gttttcggcg ggagcggcgg gccaccctga ccaaacacaa ggatcctgag gaattgcggc
     8101 tcgtggcaat caaagcattc ttttagccat ccccttttgc cctttttctt ttgtataatc
     8161 aaatcatccg aaggagaccg ccatgtcaaa tcaggttgag cattacaaaa gcctgttcga
     8221 atatgccccc atttcactct gggaggagga ttacagtggg atcaaactat tctttgaagg
     8281 gttgcgcctt ggaggcgtaa ccgacctgaa agcccacttc gcccaccacc ccaacgatgt
     8341 ggatgcctgc atgaggcgca tcaaggttac ccatgtcaac ctcaaaacat tggaaatgtt
     8401 cggcgcacga tctgtcgagg aactgatcgc caacctacac aagatcttcc gcgacgaaat
     8461 gcggcaccac ttccagaccg agctgctcgc actatggaat ggcgaaacaa attggtcagg
     8521 cgaaggcatc aactatacct tgaaagacaa accgcttaat atccgcctgc gctggaagat
     8581 cctgccagaa tgctgtgaaa aatgggaatg tgtcatggta gccatcgaag acatcacccc
     8641 gctcaaacag gcagaaaaac gtttccaaaa cctgttcaca tacgccccca tttcactttg
     8701 ggaagagaat taccagggca tccgcgagat gttcgacacg ctgcgcgcac agggtgtgac
     8761 cgatttgaaa acgcatcttc tacagacccc ggaactggtc ggccaattta tgaacaagat
     8821 cgccgtcact gatatcaacc tgaaaaccct cgaactctac ggagcgtcat ccaaagaaga
     8881 actgctcgcc aacctggacc gagtgtttcg cgatgaaatg agcgctcatt tcgccgacga
     8941 actgttcgat atgtggagtg gaaaaacaac ctacgagcgt gaggggatta actactcgct
     9001 ctccggtgaa cccttcaacg tgcatctgga ttggcggctg atgcccggac atgaagacga
     9061 tttcaaatgg gtgatggtgg cggttcagga tatcaccgcc cgtaaaaaag ccgaggaata
     9121 catgcgatac cttggcaccc acgatgtcat gactggcttg tacaatcgcg ccttctttga
     9181 agatgaacta cttcaactcg catcttctga aagcgaaccc agctttatca tgatcgacct
     9241 ggacggtttg aaacacgtca atgacactct gggacatcaa gcgggcgata acctcatccg
     9301 ccgcgcagct gaagtattaa aaaccgcttt tgccgaaaaa gccaccatag cccgcatcgg
     9361 cggcgatgaa tttgcgatta tcctgaaaaa tcctgaagcc ccacaagagt acttggatgg
     9421 aatccatgcc cttgtggctc tcaacaacaa atattatcag ggtccgcagt tgcagctttc
     9481 catgggatat gcaactcgaa gaaagaatga aacgcttgaa aaaaccctca tccgcgcaga
     9541 tgaagccatg tatcaaaata aggaaagcaa ctacaagaac cacccctcct ggcagcggcg
     9601 caaagaataa ccgccgaatc aaaaacgggt caggcttgcg cctgacccgt tttgattttc
     9661 ttttcacatc tacataaaat atttctgcca gatccgttgt ttgacctttt catccacaaa
     9721 ggaagattca aacgcatagc ggttgcaggt tgcgatatct tccatgctca tccccaaaac
     9781 ctcgtgagcg attcgatact cattgttgac attcgtctcc aacacttcgg ggtcatctga
     9841 attaatggtt acgcgcacgc ctaaatcgaa caacttcttg agcggatgct cttcaattgt
     9901 ccgcaccgag ttggtcaggt agttgctcca ggggttgacc tcaagcgtga taccgcgctc
     9961 cttgatcaaa tccaccacct tcatatcttc gatgctatga atgccatgac cgatgcggtc
    10021 tggctgaaaa ttatggatcg tatcccacac ggctgaagca ggcgtatcct cccccgaatg
    10081 aatcgtcacc ttcaaccccg cttctttcgc cttcagaatc ggcttcacaa aatcactcat
    10141 cgggtagagc aattccccat cggcaaggtc aaccgcctga atatgttttt tatggcggat
    10201 cgcccaatcc actgttttga cacaagactc tgcacccatg ccgcgtgagg taatggcgat
    10261 gatgccaatt gccatatcaa attccttttc ggcgcgttcc ttcgcacgca gcaagccatc
    10321 gaggcaggca tcccaatcca ggttgtgacc gtgaaatgcc caatcaggag aaaaacgcac
    10381 ttcaaatagt ttgatgtttt gtttcgcgca atcttcgaac aactcccaac tgatccgctc
    10441 gaaaacggca ggcgaagtaa tgctgttttg aaaaatccca aacgcctcca acaccgcttc
    10501 caaattacgc atttgatcga acaccttcac atgcgtatcc agttcgcgga catcgtacga
    10561 aggcaggctc aaatcatatt cgcgcgccac atcaatgatc gtctgcgtac ggatcgcgcc
    10621 ttcaagatgg cagtgaattt cggttttggg aatgttgtta tatttcgggt cgaagttttt
    10681 cataatgata ttttaccctg ccgccaaccg caacactgtt tgcaacagca cattcgcgcc
    10741 gttcacgcag tccgcccatt tggtgaattc gcgcggcgcg tggctggcgc catccaccga
    10801 agggacgaag atcatgccga tcgggcagac atccaccagc gattgcgcat cgtggatcgc
    10861 gccggaagca agcactgtgc tcgataatcc caagtcatca catgaattct tgaaagcatc
    10921 ttgaatctct gtgctcatta aatttggcag gtgtttgccg agcggctctg caaccaactg
    10981 caagccaaat ttttcggcca tttcttttgc aagcgtcatc aaagccgcat ccaattctgc
    11041 gagttgattt tcatcgggtg agcggaattc cagcagaaag tctgcgcggg cggggacaat
    11101 gttcgaagca ccgggcgcaa actcgatctt acccacattc gcaacgcaat tcggaaaatc
    11161 gcgcatcacg gtttcgcgca ccgccaaaat aaacgcggca acgccctgcc ccgcatccaa
    11221 ccgatcttcc atcgtcgtcg agcctgcatg gtcggcgcgg ccaatgtagc tcatgcgata
    11281 cgatccatag cccacgatcg ccgaaacaat gccgatatca atcttcgcac gctcaaggcg
    11341 cttgccctgt tcgatatgca attccaaata acccgccaaa gattccttcg cgcgctttgc
    11401 ctgaaataaa cttgcttcag ttaaacctgc acgcgccagc ccagcggtga aattttcacg
    11461 tccgctgtat gggtttttca aatcttctgg atgaagatgc ccggagatcg cactgctccc
    11521 aaacagactc aaatgcgtgc cttcttcgtc tgtaaagtca atcgcttcga gatttaaccg
    11581 caggttgatc ctgctttctt tgacagttct caacacttcg agtgccgcca tcacgccgag
    11641 tgcgccgtcg aatcgtccgc ctttggggac agagtcaagg tgtgagccaa gtaataacgt
    11701 tggaacgttt gaacgttggc aggttagaaa tgcagaatga ttccctgcgc catcggtgcg
    11761 gaattccaag ccagagtctt cgatctgttc tttgaaccat ttgcgcgcag caaggtgcgc
    11821 ctcgctgaat gtaggccgat tgacaccacc gtcacctgtc gagccgatca aagccagttg
    11881 attaaacgta tccagcatac ggtcaggggt gatgcgcaaa agtgaaaaag agttggtcat
    11941 gtggatgagt ctcctattcc cccaatcata ccgcctgttc tgcatccata tacaaaagta
    12001 gggcgagatg ctatctcgcc ctactggaaa cagggatctt acttcgcctt gatggaaatg
    12061 ctcttgggtt tggcttcttc tgccttggga agtgtgatgg tgaggatgcc gttctcgaaa
    12121 tcggctttgg ccttgtcggc gacaacatca gtggggagga tgaccgagcg ttcgaacgag
    12181 ccccagcgct gttcacgcag atgataggct ttctctttca attcatcctt ctgtttgatc
    12241 tcacctttga tggtgagcac ctcgccggtg acattgagct gcacgtcgtc agctttcatg
    12301 cccggcaggg cggctttgac cacgacttcg ttatcggtct gatacatatc caccgcaggc
    12361 acggcccagc cattgccagc gatgctgagc ggccgtgtga acgcatcatc gaagaggcga
    12421 tccatggcct cgcgcagagt catcatttca cgagcgggtt cccaacgaat taaattggac
    12481 ataggtacct ccttttaggg ttgtttggtt tgaacttacg cccttaattt aaaacgcctg
    12541 cgttaaaaaa acgcaggcgt tttgtattca ttttgttaga aatcttatgc ggttagaaat
    12601 gtgaccagtg aaacgaggcc ataaatagtg ccaaaacagg tgacgcaaat ggcgatcatt
    12661 ttggcggcag tgaccagctt attatcaggc ttctgatcgg acatggttta ctctttgctg
    12721 cgcttgtaat ctacaaagcg gtatcccacg ccgcgctcgg tgaggatgta ctttgggtcg
    12781 gcggggtctt tctcaagttt ctggcgcaga tagttaatat acaggcggac gtaatgcggc
    12841 tcatcgcggt attcgtagcc ccaaactttc tggagcagtt gatcgtgtga aacgacccaa
    12901 cccgcatttt gaacgaggtg aaaaagcagg cggtattcgg tggggcgcag tttgactagc
    12961 ttgccttcga gccagatttc gcggcggtca aagtcaattt tgaggcgttt gtcgatttcg
    13021 agcagaccat gcattgaacc cgttgcacct tcggtgcggc gcagcacggc tttgacacgg
    13081 ctgaccaatt cacgcgggct gaatggcttg gtgacataat catctgcgcc gccttcgaga
    13141 cctcgcacac gatcatcctc ttcccctttg gctgtgagca tgatgacggg cacagtagtg
    13201 atctcgcgga tggtttcaag cacttcaaaa ccatccagat cgggcatcat cacatcgagc
    13261 aggatgagat ctggcgttac atcgcgcagt ttttgaatgg cctgtttgcc gttaaaggct
    13321 tcactgacct gaaaaccatc atgctcaagg ttcatgcgga tgaagcgcac catgcgctct
    13381 tcatcatcca caacaagaat acgacggcgt tccaatgaat cagacatggt ttccttattc
    13441 cctcacgaag ctgatgatct tctcttcccc ggtgccttcg agaatctcga taaaactcag
    13501 tttggcaagc ggccagatga cttttgtgac attgttatca atatagtgca ggtctttgcc
    13561 atctttctgg cggggattca ttaccataat gatggtgtcg gtgggttttg ggagttcttc
    13621 gatctctccc gccaccgagg tttcacctga aatatgaagg atgactgtat aagacatgat
    13681 cttctcgctt tcctactcta tccgcttttt acaagtattt gtaatttcaa ctttccaagg
    13741 gccacaatat cgccatgtac aagaacctgt tcaacgttcg gggtgaggcg cttcccattg
    13801 atataagtac cattggcgga accgaggtca atgaaaatga tgcgaccggt ccctgcctcg
    13861 cgcttaatca ctgcatgaag acgggataca cctgcggcat acgcctgata gggagaaagg
    13921 tcaatatcag gcatgatggg ctgcccttcg ctgatacgcc ccatggtgaa ttcgttctta
    13981 ttggaaagcg gcaggacctg cccggtatca agcaggtgca ggctgcccca ggcatcactg
    14041 ccatcgaaag gttgataggt atcgttgggt ctggcgggtt tgctttcgcg ggctttttcg
    14101 aattgctttg ttgagatgtt ctgtgtaatg agagtatctt tgccaaccaa ctgcgacccg
    14161 cattcggcac aaaacatcgc cccatcaagg tttgtatttt tacaatttga acaaatgatc
    14221 attttacttt ctcctttgaa ccgcccatga gcaatgcacg ggtgtggtat ttaatatcct
    14281 tggtaccact ggcactccac gcatgcattt tttcaaggtt ttcggcttca aacaaggcgg
    14341 ttttggcaag agagttttca ccttttgaga ggaggtgagt tgccagatta tgcaaatgac
    14401 gggctgcttc atcatattga cctgcatcag cagcggtacg tgcccgttcc tgcattcggt
    14461 acatggtcag gcgcgaaagc gcgctcaaga tacgagtggg aggcggctcg gctgaagggt
    14521 tggagtgaac atctcgcttg aggttgatac gaatgggcgg caccggggcc gcacgggcgg
    14581 taatggaaac cttcaacata ccatttaaga cagtgacaac ttcttcatcc agcgccgaag
    14641 gctccacaat aaactcgatc aacacatgga gcggggcatc ccgcaggatc ggacccagcc
    14701 gcatgggggt ttcaatcgca attgaaccac cttcaggctg taaccgaaat gcgtatttga
    14761 ttttgatatc gtcacgtggt ttgaaatcta aaattacttc atcagcatat gtgctgatga
    14821 gggcattgaa tttatcaacc agtaactgct gaatatcctt gggcttcgag atatatgcag
    14881 atgaaccgcc tgttttactg gcaagggcat caaggaaaat atcgttccat tcgtgaccaa
    14941 tgcccatgcc tgtgatgcca atgttctggg ctgcggcttc ctcagccagt ttcaggcatt
    15001 cctgctcgtc accataggtt tgcccatcgg tcaaaaggat aatgtggttg acacgggagg
    15061 gatcaatggt gcggcgtact tcctgcaaac cagccttcag gccgttgaag atctcagttg
    15121 cgcccgatgg ctgcatcatt tgaatgcggg cttcctgttt cttcttgtca acattaaacg
    15181 cagcagggac gatcacttcg gcacggtcac tgaaagcgac cacactaaag acatcttcag
    15241 ggcgcaggct gcgcagcaac tgaacggctg ttgccttgac catatccatt ttgtcgcctt
    15301 gcatggatgt ggagcggtca agcacaaggc agatgttcag cggtggagcg ggcaggcttt
    15361 ccttatcctc gcgcggactg atctccagca aggcataaag cagctgcggc tcacctattt
    15421 tgacaaggtt tgggcggcta aatgtcacat cgtgttttac gatggtgttg acgtttatct
    15481 ccggggggag tgtggcgtcg tataaatttt ttcttttggg gttggagaga acttcgtatg
    15541 cctgttgaat ttcaagaaaa agttcggtct caccggccgc tacgttctta tccgggtgca
    15601 atcgctgtgc agcttcaaaa tatgcacgtt tgatttcctc aggtgaggca tcgcgaaaaa
    15661 cacccagagt ggcgtaatgg tccgatttgc tggacgccat gattatccaa ccatttgaac
    15721 gaggatcact gtaatattat cggggccgcc cgccagattt gccgcttcca ccatgttttg
    15781 gcaggcacgg tggagattgg gagcttctgc aatcgagcga tagatatctt tttcactgat
    15841 gacaccccac aaaccatcgc tgcacagcat caaataacct gcctgcggga aggggacggt
    15901 gaatacatcg gcttcgagtg attcgccctg cccgagcgca cggatcaaaa cgttgcgatg
    15961 cgggaaatta gcggcttcat ctttgctgag gtgtccgagt tcttccagtc gcttgaccag
    16021 tgaatggtca cgagtgatcg gttcgatcct tccatcgggg taaatggaat aggcacgact
    16081 atcacccaca tgagcaatgg tcacctgttg accgaggacc aatgcagcag taactgtggt
    16141 gccgctgccc ggcgcttcac gctgaacgta ttgatgggct tccatcacag acgattccat
    16201 cacctcttgc agggattcct gaagagcctg agtaggcaaa ttgtaaaggt aggaatggaa
    16261 ttttttagtg atgttgccgc ccatgatgcg tacggcagca ttgctggcta cttcaccaaa
    16321 ctggtgccca cccatgccat cggcaacgat atacaaccca aaagggatgc tttctgtgct
    16381 accggcaatg gtggatgtga tggccaacag actatcttcg ttatgttcac gctgttttcc
    16441 aaccgactgg cccacactgg caatgacctg tttcgtttca tacctcacat attgattcgc
    16501 aataatggag ttgatctgct ggtcggaaag cggcgcagtt gtggccatat caacctgctt
    16561 tggtttggca ggttcctcag tcggttttgt accaaatagt tttcggaaaa aatcagccac
    16621 gagagactcc ttttaagcga acaatgcata aacttgatgg tctgtagaac caaaataaac
    16681 aatatcatcg aacacaatgg gcgaaccggt aatggcggcg gctgtttcaa acttccaacg
    16741 caatcgacca gtacgatatt ccaggcagta caaactgcca tcaacagagc cgcaataaag
    16801 cgaatccttg taaattacgg gagaaccgct gacctgattt tccgtacgga aacgccagac
    16861 ttccttggca ttccgcgtat caacgcaata gagaaatcca tctgcagcgc cgacgaaaat
    16921 aaaatcatct gcaatggccg gggaggaaat ggaacccttc cccatgcgga acttccagat
    16981 cggccagccg tttttcgaat ccagcgcgta caaagtgccg tctacagatg caaaataaat
    17041 cgcctgcccc ttgataaccg gcgaagaggt gacggctcgt ttggattgaa aacgccattt
    17101 ctgctcaccg cggaaatcga cggaataaaa tccgccagac tcgttgccaa aaaatatcat
    17161 ttcatttgca accaacggcg tggaatgaat gggagagtct gtggcaaatt tccatttggc
    17221 gcggccactg gtgatattga ccgcatgcag gtcctggtca tcggagccga agaaaatatg
    17281 gccatcggcg atacgcggcg aagagtagat ctctccttcg gtgtaatacg tccacaccac
    17341 cttgccgctg cgaatactga caacatgcag gcgatgatct tccgagccaa aaatgacatt
    17401 attgtcaaat actgttgggc gcgaaacaat gccgccttca gtggcgtact tccactgaaa
    17461 ctgaccatca gccgcattca aggcgtacag gttgttatca tagcacccga tgaacaacat
    17521 gccctgatgc agggccgggg tgccgcgaat ctcatcttca catttgaagg tccacagcgg
    17581 tttgacacca ccgctggata ccggcaaagc agctgccact ttttcgagag tacccgtctt
    17641 gcgcgcaacg tttagaaggg catccttcat cgctgcagtg ctggaaaaac gatcaccagg
    17701 gttgtattgc aaagccgtgt ttaccaccgc ttcaaactcc agcgaaacat tggggtttat
    17761 gcggcggatg ggacgctcgg caaatgaaaa aggcggttca aggcgcggat cacgacgggt
    17821 gatggcatga tgcagggtcg cgccgaggga ataaatatca gccaatgggg tggcttcacc
    17881 acgatactgt tccggtggag agtagccttc cgtaccgatc atggtgcctt tttgcccgga
    17941 ctgaaacgtt ttcgcgatgc caaaatccac caacaccaca tctccattgt gattgatcat
    18001 cacattggaa ggcttcatat cacggaagat gatggggtct ggtttgtgac catgaatgta
    18061 cgccagcacg tcacagagtt gaatggccca gctgatgatc tggtcttcag gtaagaaacc
    18121 gttcgaatca ccgatgaccg cttcaaggtc cttgccgtgg atgaactcca gcacaaggta
    18181 agagcggtca tccagtgaaa aataatcata aatacgcgga atggcagggt gattgagcgt
    18241 ggcaagcaaa tttgcctcac gctcaaagtt ttgcacgatc gtctgacgca caagcgggtc
    18301 tggcgcgctg ttgatcattt ccttcacggc caccaatttg accacgttcg gaaagtgcag
    18361 atcgcgggca cgataaaccg accccatgcc tcccacgccg atcacatcct ggatgttgta
    18421 gcgatccacg agtgtaatac cagattgtat ttgctggcgg gaggcggaat ggctatcgcc
    18481 tgaatctgac cctattgggg aagtgatgtt tctggtttcc agagaggact cctgagcgtt
    18541 tcgtactggt tcttcgatta tagtgtgcag ttaatggaat tgcaaatgct ggaaattgca
    18601 atttcgtcgg taaactggct tgcgaggatt tacgttctac ggcaaaatgg aaaggctatg
    18661 aagattctcg cagtgagcga ccaggccatc gaacggctct acacactttc agccagcggc
    18721 cattttcagg atgttgaact gatccttggc tgcggagata tgccattcga ataccttgaa
    18781 tatctggtca caatgctaaa tgttcccctt ttttacgtac cggggaatca cgacccgcaa
    18841 cacaacccac gacaggaatc tgtgcgaccg gaaggcggcg tcaatcttga cctgaaaacg
    18901 gtgcgccaca aaaaattcct gatcggcggc ttcggcggaa gcattcgata ccgcccggat
    18961 ggcgtcaacc aatacagcca gcaagaggcc tactatcgag ccttcagcat gctcccccgc
    19021 ctgctggcat accgcatcca atatggccgc ccgctggata tccttatcag ccattcgccc
    19081 ccgtttggta tccacgatga ctcagacccc gcccatcagg gcctgaaaac cctcaactgg
    19141 ctgctccgca tcacaaagcc gcgataccat tttcacggcc acacccacct gtaccgccgc
    19201 aacctggaga acgtagaaac caacatcggc ctgaccaaag tcattaatgt ttatccatat
    19261 aaaaccatag aagtgagcca ctagtcatgt ctgacgaatt aacctcaaga gtgcgtgctg
    19321 atttcagcag agcgcgcttc aaatccttta tcaatcaggc gattgccgtt ctaaaaggcc
    19381 agcgccccac caccctgctt tcctacgatg atgtcaagga aaagctgcat atcggtggtc
    19441 ccatttatcg gggagtaaaa accattcgcg tggagcagat cgcgggtagt ttgaatcgct
    19501 atcacgaatt tgaccgcgct tttcttccgc gtgaagacca gcttgccagc cgctggcaaa
    19561 aagtcgaccg tgccttttat caggatgtga atctcccgcc tgtcgtgttg tacaaagtgg
    19621 gcgaagtgta tttcgtcgtt gatggtcatc accgcgtttc agtggcgcgt gagcagggac
    19681 aggaattcat tgaagccgag atccgcgaat gtgccacccg tgtaaacctc acaccaaaca
    19741 tccagcccga agaccttgaa attctcggca ccaaagtgca ttttctggaa cggtcctcgc
    19801 ttgaccatat ccgccctgaa gcgaacatca aactaaccat tcccgatggc tttgaacgca
    19861 tgctcgaaca catcgccgtt catcactatt ttatggggct ggacttgaaa cgggatgtca
    19921 cggaggagga agccgtggca cactggtacg atacagtcta catgcccatc attgacgtgg
    19981 tgcgaaaaag cgacatcctg aagaacttcc ccgataaaac cgaaggcgat ctgtatttgt
    20041 ggaccctgga tcatcaacat tatttatcga aggaaaaagg ccagccgtta caaccgccac
    20101 atgaagccgc acaaagattc atcagaaaga agaaaaagaa ataaccatga ccgatctaca
    20161 tccacttgca ggagtctacg ctgcagcagt gactccccta ctcgatagcc ctgccaaagg
    20221 aagcaagctc gattcaaact tcgaccttga agctgttcct gctcttttgc gtcatctcgc
    20281 cgccagcgga tgccacggcg cactgctctt cggcaccaca ggcgaaggcc catccttctc
    20341 tccaaaggag cgtgaggtcc tgctgcgttc cgttcgtgtg taccgccaac agctgcctgg
    20401 gttcaaattg ctggcaggca caggaacacc aagcctttcc gaaacgatcg aattgaccaa
    20461 actgaccttc gatctcggtt atgacggtgt ggtggtgttg ccgccatatt atttccgcaa
    20521 agcagcagac gaaggtctgt tcaaatggtt cagtgagttg atcaccaaag ccgtgccagc
    20581 ccataaatat ctacttggtt atcacatccc cagcgccgcg ggcattggtt tctcacttga
    20641 tctgatcgcc cgtttgaaag atgcgttccc aaatcaattt gcgggcatta aagactcctc
    20701 gcatgatgag gctttttcgg cggcagtggg tcaacgcttc ggcagagact tgctcgtact
    20761 caatggcacg gattcgcact tccaccatgc attgaagaat catgcccaag gggccatcac
    20821 cgcaatggca aacctcattg gcagcgatct gcgcgatatc tgggacctgt acaatgaagg
    20881 tggggatcca tctgaaattc aggtgcgggt tgcaaaacaa agacacgccc tcgaaaagta
    20941 cactccgttt ccgcccactt tgaaagcatt actgcacaaa ctacactccc tgccacggtg
    21001 gacggtccgc ccgccgttgg aagatatttc acctgaactt gaagatcaaa tcctgcaagg
    21061 gtgggaaagt ttgcaggttt aaatgtttac aggttcaccc tctccaactt tcgaactttc
    21121 caacctttaa accaaaatga actggcgtct cctttacact cccccatcca gcggtgcatg
    21181 gaatatggcc gtggacgaat ccatccttga acacattcac cgcggagaat cgctgcctac
    21241 cctgcggtta tattcatgga atccaccctg cctgtctttg ggccacgcac aatcctttgc
    21301 agatgtggat gtggaaagat tgcgggcaaa cggttgggaa gttgtccgcc gtgtgacggg
    21361 ggggcgtgcc attttacaca ccgatgagtt gacctactcg gtcacaggcg gtgcagatga
    21421 acccgtgctg gctggcggcg ttttagaatc ctataatcgg cttgcacagg ctttgttgtt
    21481 tgccatccgt gagttggggc tgcctgttga aatcaaggaa catatctctg aacctgcaac
    21541 cttaaacctt aaccccgtct gctttgaagt gccatccacg tatgagatca ctgtggaagg
    21601 aaaaaaactc atcggctcag cgcaggcccg caaaaaggaa ggcgtacttc aacatggctc
    21661 gctcccactt acgggagacc tgacgcgcat ttgtcaggct ctggtcttca gggatgaaac
    21721 tgcgcgggca agtgcagccc aacgtttgct ggcacgggca acgaccgttg aatccacttt
    21781 gggggtgaat cctgattggg aaacagcagc ccaggccttt gttaaaggat ttgaatcgca
    21841 gttgggaatc cactttcagc gtggagaatt gtctgcatcc gaatctgaaa gagcagagga
    21901 attaatccag gaaaaatatg ccaacccgtc atggacggaa agatcatgaa aaatttccta
    21961 atctatgggt cttacgggta tacaggcagt ttgatcgttg aacaggcagt caaagcgggg
    22021 atgaagccat tacttgcggg gcgcgatgaa aatctattgc gcacacaggc tgaaaagttc
    22081 gggttggaat atcgcgcatt ttcaatggat cattctgctg cgctcgacgc agctttattg
    22141 gaagtaaatg ccgtacttca ctgcgcgggg ccgttcgtct tgacataccg ccagatggtg
    22201 gaggcatgta tccgcaccag gcgacattat gtggatatca gcggcgagat tgaagggttt
    22261 gaagcattgg ctgcaatgga tgaagaggca aaacgagttg gcatcatgct gctgcctggc
    22321 ggcggcttcg atgtggttcc ttcagattgt ttgattgcac atgttgcaaa aaaacttgca
    22381 aacccaaccc accttgagat ttacatcaaa agcattggca gcggggtctc acgcggtacg
    22441 gcccgctcgg gtatcgagaa tatgcagcgg cagggacgca tccgccgcaa tggagagatc
    22501 gtgtctgtgc cgaatgtttg ggatagcaag caggtggact ttgggcgcgg tccttcgcgc
    22561 gtggtttcag ttggctgggg ggatgtaagt acggcttatc acagtacagg cattccaaat
    22621 gtgacagcat acatgggctt ccccgctgca atggtcaatg ggatgcgcct cacccgccat
    22681 cttggatttt tgctttacaa ccgcgctgca aaagatttca tcaaatggct gatcggggtt
    22741 tttctcaaaa caggtccatc acgccagcaa aacgagaatg gcttcagctt gatgatcgcc
    22801 gaagcaacga atggaaaaca gacagtccgt gcaaagctgc gcacccccga agcctactat
    22861 ttgaccgcat taacagccgt ggaaatcatg aaacgcatcg cttctgaaga tttcaaacct
    22921 ggctttcaga ccccaagcaa agcctacggc tcagatttca ttttacagtt tgaaggggta
    22981 acacgcgacg atttgtaatg tgatacttca gcatgtttgt tccctaaaac aaaaaagccc
    23041 taaaggtttt gaaaaccttt agggctttgc tttcatcgtt tatcttcgat ttctcaagaa
    23101 ggtcgggata tccagatcat cgttgctgat ggagggctga gacgcaggct tggattcgcc
    23161 aggggtcttg taatcggcgc tgacgctgac cgattcgctt gagcgggcga acaatgcact
    23221 ggctgaagcc gcggtcggtt tctcagtgcg ggcggcaggg cgttccaaag cgcggcgcgg
    23281 aacgctggtg cgttcaaatc cggtcgcgat gaccgtacag cggatctcgt cacccatttc
    23341 aggatcaatg accgcaccga agatcatatt gacatcgggg tgtgcggttt cgcggataat
    23401 ggctgcagcc tgattgacct cgaagagggt catgttcgag ccgccggtca cattgaagag
    23461 gacgccgcgt gcgccgtcaa tggtgatatc gagcaattga ctggaaatgg cctgctccgc
    23521 agcgatcttg gcgcgttcat caccagaacc gcgaccaaca gccataaggg cagcgccgcc
    23581 ttcagacata atggctttga catcggcaaa atcgaggttg atcaaaccag ggatcgtgat
    23641 gagttcggaa atgccttgaa taccctgatg cagaacttca tcggccatgc ggaaggcatc
    23701 ctgcagggat gatttcttgt cggccagttg aagcaggcgg tcattgggga tcgagatgag
    23761 ggtgtgggcg tgttccttca tcttcgcaac gccagattca gcagactgtg tgcgcttgcc
    23821 gccttcaaag gtgaaaggac gggtgacaac accgatggtc aatgcgccac attccttggc
    23881 gacctgcgca acaacgggag cagcacctgt acccgtacca ccacccatac ctgcggtgac
    23941 gaagaccatg tctgcgcctt tgagaacatt gtagagttca tcagaggatt cttcagcggc
    24001 tttgcggccg atctcaggat cgccgcctgc gccaagcccg cgtgtgagtt tgtcgccgag
    24061 gcgcacacgg atgggcgcgc gtgaaagcgt caacgcctgc gcatcggtat tggcagagat
    24121 gaattcaacg ccctgaattc cctcttccat catgcggttg actgcattct gaccagcacc
    24181 accaacgccg atcactttga tgcgggcgaa ggcttcggta tttggatttc ttttgttcgt
    24241 gtccatgtgt tcctcctggc aaggatatgc caatcttaaa cgagaatgac gatttttaaa
    24301 agggtttaaa caatctttgt gaatcttctc attaaacttt ctttccatgt ttttggaaga
    24361 aagttaacaa aactagggta acagccgctt catccaattc ttgatcgaat ccaaattcat
    24421 tgaactttct cctctcgatc tgcggcggtt cttgttgccg cttgtaacga tcacatcatg
    24481 atcgcgcatg gttgtggccc aacgcagcaa gccaacgctt gtagagtatg caggcgaacc
    24541 aagtttatca accagaccaa caaggttttc cggctgagcc gtacgcaccg gcatgcccag
    24601 cacttcactg gcaatttgct tgatacctgg caatgcagag gtaccaccag tcaacaccat
    24661 gccagcgggg agcaaaccat cgtagccgga gcgtttcact tcctgcagaa tcaggcggaa
    24721 ggtctcttcg atgcgggctt cgatgatatg agcgagatcc tgacggttga tcttcacatc
    24781 gttatcctcg ccaaaggggc gaatggagaa atattcatcg ctggcaatat cagagcggat
    24841 ggcatatccc tgttgctttt tcacttcttc ggcctgggcc atgggcaggc gcaaaccatg
    24901 tgcaatatcc tgtgtcacat gatttccacc gacggccaac accatggtat gccagacatc
    24961 gccattcaca taaatggcca agtcggttgt accgccgccg atatcacaaa ccgcaacgcc
    25021 catctggcgt tcctgttccg tcaacaccac ttcagcagat gccagcggat ttaaaacaaa
    25081 ttgctgaatc tcaacaccag ccgcgccgac acattgacga aggttatcca ccgtggctgc
    25141 cgctgcagtg atgatgtgcg tttcaacttc cagcttgtaa ccatgcatcc caatcggggt
    25201 gcggatcccc tcctgcccat ccactgtgat gccgcgctga atgacatgaa cgatttcgcg
    25261 gtcatgcgga atggccaccg cttgagcctg ctcgagcgca cgggcaatat ccattgcatc
    25321 gataatgcca ccggggattc cagaagcgcc gcggctgttc acggaagaaa catgcgcacc
    25381 tgccaggctg accagggcag tggtgatctc caaaccggaa gtgttctcag ctttttcaac
    25441 cgaacgttta atggcttgcg atgcggcact gaggtcaaca atgatccctt tgcggattcc
    25501 gtccgagggt tcgatcccta cgccaagaat ccgaatggtc tttgcatcct ccacccgccc
    25561 gacaagggtg caaattttgg ttgtgccaac atccacgcca actacgattt catccatctt
    25621 tactgttgtc catcttccac aataattgca tcttccgatg ctgcatcggt ggcatccagt
    25681 tcgagatcag ccatgcgata aaacggcgca tcaggataca caacgctgat aaatacggga
    25741 acggtgccgc ggcttgtcaa agatgcgacc agggattgat acacccgaat cttcaatggc
    25801 atgtcatggg cactgattcc aaaataggcc ttccaaccgc gttcatcctc ccagccaagg
    25861 ccgttggcgg catcataaaa catcgttgca ccggcaggca cattgggggc aaggatgagg
    25921 atggaatcca cgagttcttt cgaaacgtag ggcatcgggc tgagcggatc ttccgtggca
    25981 ggctgaccag ctggcggcgt ggaaagaccg ttcaccggaa cgagagtcgc aaccatgccg
    26041 cgcggacgaa aagcaacgcc agacgcatcg atccatgtaa atccaccgcc ctgctgccag
    26101 aggatgacag gctcgcgctc cactactgta acgtaaacat ggttcggcaa ataagcatgg
    26161 atatccaccg acgaaagttc cgggtaattc atgcgcagac gaacttccaa ttcatcaggt
    26221 tgaactgtga aaatggactg gccgttcaca gcaagcactg cttcgatctc ttcgcggctc
    26281 aggcgccccc cacccaacag ggtgatgttt ggcacgtgga aatacggcag ggtcaacgaa
    26341 aggtaaatga ccacgcccag caaaacaacg agaacaagag atgtcagccg ccaactgggg
    26401 cgaatgcgtg gcatgtggaa ctggggtttg tgcaggtgaa tttccggcag acccagtgca
    26461 atgttaaagc ggcggcgcgg cgcggactga cgagtcaatt tgggagcaac cacgatctca
    26521 ggcacacgag aggtcactga ttgcatcggc ttcagggcgc gcttggtggt caactccagt
    26581 tcttttctgg cgcgttgatt tcggcgctga cgggcaagtt cagcacgggt ggggcgttga
    26641 cgttcgctca tgctgtcctc cgtcgctcgg tgtgatcacg ctgggccttg cgctcgaagg
    26701 caagttcgat caggcggtcc acaagctgcg gatagttcaa accgcttgcc tgccacaact
    26761 tcggatacat gctgatggag gtaaagcccg gcagggtgtt caattcattc aggtagatct
    26821 ttttcatatc gttatcaaaa aagaaatcgg cacgtgccat gccagcacaa tcaatggcct
    26881 tgtaggcacg ggcggcataa tcacggatct ggtcactcac atcggctggc aaaatcgcag
    26941 ggatgatgag gccagaggtg ccgtcaatat atttggattc ataggagtag aactcacggc
    27001 tgggctgcac ttctccgcat acggatgtct gcgggtattc attgcccaaa acgctgactt
    27061 caatttcacg cacattccca actccgcgtt caacaatgat gcggcggtca tatcgtgcgg
    27121 cttccatcaa accttcgccc aggtcggaac gtgaattgca tttgctaatt ccaaccgaag
    27181 acccgaggtt ggcgggtttt gcaaagaacg gataagcgcc catctgctca actttggcga
    27241 tgaccgcgtt catatccttt tcaagctcgt cgcgcagaat gaggatggaa tcaacgatgg
    27301 ggattccgtt tgcgcgcatc acatccttga aaataccctt gtccatgccg accgatgagc
    27361 cagccacccc cgcccccaca taggccacat ctgccagttc aagcagccct tgaattgtgc
    27421 cgtcctcacc aaacggacca tgcaaaacgg ggaagaacac atccaccccg gagagtgttt
    27481 caagtttctc gccaagcttt gtcgaacgga tgacatacaa agattgatag gatggctccc
    27541 acggcggtac aacacggtcc agcccatcca cgcttccatt ttcaaatgca tccaacgcat
    27601 ttggcccggt gaaccaatca ccgtccaacg tgatgccgat ctggatcact tcgtagcgtt
    27661 ccgcatccag agcattgagc acggagcgcg cagacatgag tgaaacatca tgttcaccgg
    27721 aacgaccacc aaataacact gccactcgaa gtttcttcat atcgtttcaa gaacaaacgc
    27781 tttcgcggtt attcgttcca atctccaaca agctcaactt ccaactccaa ctgcacgccg
    27841 aatttttcgc gcacagtttt ttgcgtcaaa cgaatcaagg cgagaatatc cttcgcccgg
    27901 gtctcgccgt gattaatgaa aaaatttcca tgcaaggtgc tgatctcagc gttgccgata
    27961 cgtacacctt tcaagccagc agcttcaatt agacgacctg catgatcacc ttcagggttc
    28021 ttaaacatgg aacccatgct cgcaccaggc ggctgcgtgg ctttgcggcg ttcgctgaat
    28081 tgctcgattt taacagacac ttcctctttc gctgaatttt ttaatctcag caaagctgaa
    28141 agtacgatcg catgaatttc accgcgcttc aatacgctgg tgcgatatcc atatcccatt
    28201 tgttcaacag acattcgttc acgtccgttt tttgtcaaca catcggccca gatcaaattc
    28261 atcgacacat cgccgccgaa cgcgccagca ttgccataca ctgcaccgcc tactgtgccg
    28321 gggacagtcg ccgcccattc caaacctgcc aaacctttgg atgcacagcg attggcgagg
    28381 ttgctgaaaa tgacacccgc ttcacaccag accgtgggct ggtcgccgcc ttcgaagcgc
    28441 acttccttcg cccgattcaa aaccaccacc ccacgcagcc ccaaatcact gaccagcaca
    28501 ttcgacccgc cgccaaggat gtaatatgga aaatcatttt cccaaatgac cttcattgcc
    28561 tcggcaaact cgtccgccga tttgaccgtc aacagcacat ccgccgggcc gccaatacgc
    28621 gcagatgtgt acggcgcaag agagatattc tcctgcacgg catcgccaaa gatctggcgc
    28681 aacacatcaa catgaagggc ggaacgggcc atatccattt ttacagtttc ttcttttcca
    28741 aatgttcgat gatcttttcc aaagattgct tcccgacatt ctcactgccc aacaccggaa
    28801 ttgcagagag tttgcgggtc gcgttgatca gggcggcctt tgtcaattgc tcatccaccg
    28861 cgtggaacag cgccattttc aaaggcggga gcgcgatcac ttcataaaga tgagccattt
    28921 gcttttcgtg attctccaac atggtcatct cacaaattct tcaacacatt cgtactgatc
    28981 tggtcggcat ctcctgccga aaggaccaga agcacatcac cggaacgcag gttcttcagc
    29041 aggtatgcgg tcgtatcttc cagtgaacca ctgtaacgtg ctgatgcatg cggcatggac
    29101 ccaaccacct ccgccgagga aaattcctgt ttggcctcgc gtgaggcata cacctcggtt
    29161 accagcactt catccgcatc gctgaaggcg cgggaaaatt caaagaacag agtctgtgtg
    29221 cgcgaataag tatgcggctg ccaaaccgcc cagatgcggc ggtcaggata acgagccttt
    29281 gcacccgcca gagtggcacg gatctcagtg ggatgatggg cataatcatc gatcacaatc
    29341 acacccttct gctcaccgcg aatctcgaaa cggcgtcccg tgccggtgaa ttcaccaaga
    29401 gcgtctgcgg cttcttgaag aggcagaccc agcgtagcca tgacagacaa agccgcaagc
    29461 gcattgcgga cattatgttc acccggcacc tgcagtgaaa cattcaccac cgaagccgat
    29521 tcggtgccca tgtttgtcat tgcggagaaa tcaaaaccgc cacgggtatt gggcttggga
    29581 gtgcgtgcct gcatccattg cggactgttg atggtcaact cgctttgtac gctgtaagag
    29641 atcacactca ggcctttttt acgtgcatag gaaagcaaac tgaccgcgcc ttcatcttcc
    29701 gcacagacaa ttaatgtgcc gtcttcagga agcaggtcaa cgaagccttg aaaagcagaa
    29761 tacatgtcgg caaaggtcgg gtaacaatcg gggtggtcat gttcaaggtt ggtgaccacg
    29821 gcgatctgcg gcttgagacc gaggaacata cggtcatact catctgcctc gatcacaaag
    29881 ggactgccct tgccagcacg ggcgttgact ttcaggttgt tcaacgtgcc acccacaata
    29941 aaagatgggt cacgcttcaa ggcagtcaag gtccatgcga tcatggcggt ggtagtggtc
    30001 ttgccgtgag tgcctgccac tgcaataccg gtcttttcag acatcaactg cccgagaaaa
    30061 tcagagcgct tgtaaacagg gactcctgca cgtttggcgg cttccacttc aacgttttca
    30121 tctggaatgg cggaggaacg gatcacaata tcggcacctg aaacattacg cgggtgatgc
    30181 ccaacgtaaa tgctgacacc gttattctgc aaatccactg cgaacggtga aagcgtgcgg
    30241 tcagaacccg tcacctcgta gccgctctcc ttgagcaggc gcgcgatggc ggaaagtcct
    30301 gaacctccga tgccgatgaa atgaacacgt ttcataattt agctcctagc catttaccga
    30361 tggcgattgg aatgcagccg caagccaacc gctcacagcc aatcgctaga tatacaccac
    30421 aataatgaac accaaaacga tgatgacagc gaagtatata ctcggttcgc ccaaaaagcg
    30481 cgggggcata tatttcatca tcgtttcaat tgcctgagcg atggccgggt ctgtggccgg
    30541 gctgataatg ttcgaatacg ggtagagggc catgtggtta tagaaaagaa ttgaccctgc
    30601 caccaggtag ccattgaaac cgcccagcac ggcgccgaac atcccatcct gcagtttttc
    30661 gcgcgcggct ttgcctgaaa aacgcgtgag gttgaccgtt tgatacccaa aaaacaccag
    30721 tgtgatcaaa ataaggctgc ggatccaaaa catggaactg gatgccttat cgagatcgcg
    30781 gacaaggggg atgtattttt ccaacagcaa attcacagcc agtgcagtaa ttacgctgaa
    30841 gatgaccatc aactccttgg cccatccgcg catggcgccg acaatgccaa agagaatgat
    30901 atacatccag aagatataaa caacgctcat cataaggttt atgctcctgc caatttaacc
    30961 aactggctgg caatactgcc tgccgcatcg gggtgtgata aagcctgcat ggcggcacgc
    31021 atggattcga gtttggtttt gttcaacagt aaacctttca caacagatgg caggttctct
    31081 tccaggagtt catcctgcaa aaggactgcc gcattctttt caacgaggaa atcggcattc
    31141 actttctgat aacgccatgc atatggatac ggcacaagca cggcgggcag accaaagaag
    31201 ggatattctc ccagggaaga agcgcctgcg cgtgagagga ccaggtctgc ggcggcgaga
    31261 gcggcgccca tttcatgtga gtagggcatg gcatgatacc gtgactgtaa atctgcaggc
    31321 aatgtctgtg cggctttctc aatgacgggc caatccaacg agcctgtgat gtggatgacc
    31381 tgtgctgcgc cgagcaattc atggaggtgt ttcaatacag ccatattgat cgaacgcgca
    31441 cctttgcttc cgccggtgac aagcagggtg ggcatttgtg gatccaaccc aaagtgctgg
    31501 gttgcttttt cgcgggacca gtttgaaagg tcagagcgaa gcgggtagcc ggtgaggacg
    31561 agtctttccg ggcggttgaa atattttttt gagtcaggcg ctgttacagt gacgacatcc
    31621 gcaaaccggg aaaggaattt aagcgcaagc ccgggttcga tatcgggaac atacaacgcg
    31681 gttgggatgt ttcttccagc aacggccatg ggggcagcga caaagccgcc ggtgaagaag
    31741 agtacatcgg gtttgaattc acgcaagatg cggcgcgagg cttgcacacc gcgtgtgagt
    31801 ttggcaaggt tgcccggcag tgcgcgcagg cccacgccat gcacacccgc ggcagggatg
    31861 gaacggaaag ggatgcccgc acgattcacg agttcctctt ccatgccgcc ttcgccaccg
    31921 acccagagtg tttcaacttt ctggcgctga ctggttagcg cgttgtgtac ggcgagtgcg
    31981 ggataaaccc cgccgcccgt tccaccagcg caaattaaca accgcactgt aagacctcca
    32041 ttcgtcatct gagatcgtgc cttcgcctgt ctggcgagag atgttaaaaa gaattccaag
    32101 cgacgccatt gtggcgacga gatttgaacc acctgcgctg ataaaaggca gggcattgcc
    32161 tgcgaaaggc agcaggccca ccatcaccgc catgttgacg agggcttcga tggcgatcca
    32221 tgtgaccaac ccggtggcaa gcagtgtgcc aagcatgtcg ggcgcacgac gagcaatgac
    32281 cattccccgc cagaccagcg caccgtacag aacgatcaac cccactgcac cgaaccagcc
    32341 aagttcctcc accaccacgg cgaagacact atcggtggga ggcacaggca gcccggtaag
    32401 tttggattgc gattggccga ggcccacacc aaaccaccca ccattgacaa tggcttcaaa
    32461 agagcggcgc acatggtacg aggcctgcaa aggatctttg atacctgcaa taaaatcacc
    32521 cacacgctgc tgacccgtgg cgctgatgga agcaacgatc catgcggcaa cggttgcaac
    32581 gacaagcagc acaccgatct gcttcaggtc gccgcccgca aggaagaaga gcagcccgcc
    32641 cagcataagc acagtggcgg cagcgctcag gtcgggttgt tggtagatca acccacccac
    32701 cacaccgagg atcacgccca gcgggatcaa gccgagggaa atatcgtgca ggtattggcg
    32761 cttggcatac aaccaaacgc tgagatataa aatcgaaacc agctttgcca gctcggaagg
    32821 ttgaatggaa ccgccaagga tcgcgcgttt ggcgccgaag cgaatttcat tcatcaacag
    32881 cacaacgacc agcagaccga tggtcagggc catggcaggc acaacatact tgcgccagtt
    32941 gtgataatca aagaaagcca gcaccagcgc cgagaacaac ccaagcccaa gccaggtcaa
    33001 ctggcggttg aacatgtaca ttgcatcgcc gtatgcaccg agcgagaaat cccatgaaga
    33061 ggaatacaac atcgccagtc cgaacacgat caaagcaacc accaccacga gcagcggcat
    33121 atcgaaacca ccggtgaagc gcggcttttt gtaagtctgt tcgtttacga aagttcctgt
    33181 acccattttc taaaggcctc tcctctctct gcaaaatctt tgaattcatc gtaacttgtg
    33241 ccgcccggtg agaacaggac gacatctcct gccgtggcag cttcggcagc aagggccacc
    33301 gcctctttaa gatgcctggc gcgacgaaga tcaacgcttc gctccccgcc gacagctgat
    33361 atggctttct gaatcagttc cgccgcctca ccgaagagca ccacatgatc cacacgtttg
    33421 tgaatcagtt ttgcaatttc atcccagggc aggtctttat cacgcccgcc gagcatcaaa
    33481 acgatcggat catcaaatga ctggatgcca gccatggcac gttcgggcgc agttgcgatc
    33541 gagtcgttga accagcgcac cccattcctc tcgcggacaa attccagcct gtgtgccaca
    33601 ccgcggaatt cttcaacgac ttcgagcatg gcatccaatt tgaatccggc ggcatgccca
    33661 atggtgaagg cggccagcac attggctaca ttgtgaaggc cgggtaattt gatcttctcg
    33721 cgcaacatca aaggcaggta ggcatagcca tcacgcaggc tcaacaagcc atcctcatgc
    33781 aggtacgagc cattgaaacc ttcgtccaga tcatgcaggc tgaaggtcat cagtttgcct
    33841 ttgaccttgt tgcgtaaatt ccacgagcct ttgtcgtcgt ggttaaggat ggcggtatct
    33901 ttttcggtct gaaattccaa aatgcgcgcc ttggcattgg tgtaggcttc cattgtgccg
    33961 tggcggtcca aatgattcgg tgtgatattc aaaatagccg cgatgttcgg tgagatcgtc
    34021 atctgctcca attggaagga tgaaatctca agaatggcaa ggtcgtcagc ggtcatatca
    34081 tccacataat tgatgagcgg atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnngaatatc tgcgtatcgt tcgaaagcgg aatgccgcgc ttgactgctt cctgcacaat
    34261 gggcaatgtc agcggcacgc cgccggagag gcaaagcaca tcggtcgcgt ccagcaattc
    34321 cagcggatga ccacccgccg cccaagttac atccgtatcc gcaagagatt cgcgagcaga
    34381 ggcaagctgt tcaagtgaac gcgaatcgct caaagtcacc tttgagccgt ggcgggagag
    34441 ccagcgggca agggcgattc cctgccgtgc tgctccgaga atgagggtgc gggtcgagtt
    34501 ccagttggtc attatggttt gaaggttgaa agtttaaggt tacaggttaa gaaaacccgt
    34561 cgcctattca ctattcacca ttttctatac ttgcgacaat cctatgccga tcaatgcggc
    34621 catgaggcca atgagccaga agcgctgcac aacctgtgtt tcgctccagc cgagtaattc
    34681 aaaatgcagg tggatgggcg ccattttgaa aaaacgcttg ccctttgtcc atttgaaata
    34741 accgatctgg atcaccacgc tcaacatttc gctgagcgga ataatgccaa tgaccagcag
    34801 catcagccac tgcccttcca tcaaggccac tacagccagc actgcaccga gtgagagtga
    34861 accggtatca cccatgaaca attcggctgg atgaacgttg aaccataaaa agccaaaaag
    34921 cgcgccgacc aaaatgaaac aaaaacgcgc aagatagacc tgttcctgca tcaaggcaat
    34981 gccgccaaat gcggcaatgg cagtggcggc gatcagccct gccagcccat caagcccatc
    35041 ggtaaagttg acggcattcg attcggccac aatgatgaat gcggcgatgg gcacatacca
    35101 ccagccaagt tccacttcaa aaaagagacc ggggaagtac atgtccggcg catccatttg
    35161 atatttcaac acataggcgg ccgccagagc cagaatcact tgaaacatga acttggtgcg
    35221 ggcacgcatg ccgtcacctt tccgtttacc gcgaatgcct tcccaatcgt ccaccgcgcc
    35281 gaggacggca aaaccagcca tgaccgccat cggcaaaagg atggaacgcc ccacccccac
    35341 cacatcggaa cgaccgccga tcaacgccac agcgttgagc aggccgctaa acagcaacac
    35401 cgggaaaatg aacatcacac cacccatggt gggagttccc attttcacac cgtggtgacc
    35461 aggctcctcc acgcggatga tcttgccgat cttgtaatgg cgcaaaatac ggagcagagg
    35521 gccaccccaa atggcggtca ttacaaaagc aaggcctgcc aggctgagtg atagggcgat
    35581 ctgtctcacg attgagtctc caaagcggcg gtaatgcggt ccatgcgtag gccgtgtgag
    35641 cccttgatca gtacggcatc attcgaggtg aggtttttgt tgagccaatc gatgatcggt
    35701 tcagaatttt cgaattccaa aatattggca gacttcatac ctgtgcggcg tgcagcatct
    35761 gcgatcatat gcgcacgcgg accaagggta agcatgacac gggccacctg tgcggcacgc
    35821 attccaacca tttcgtgtcc ttgtttctcg tagggtccca attcaagcat gtcgcccaac
    35881 acagcgatct ttcgtccatc catttcatcg agcaggttga gcgctgccag catggattcg
    35941 ggtgaggcat tgtaggtgtc gtccaaaatc aaagctccgg tattgctgcg aacagccaca
    36001 aggcgcaact gggcatgtcc ctgcaagagg ccttcgaaaa tctcctgcca ggtcattcca
    36061 tcattcaagc ccactgcagt ggcacgcaag gcagtgtgaa ccgaatggcg cccgatcatg
    36121 ggaatgtgcg aatgaacggt ttcacctttg tagtgcaagc ggaagcgaat gccatccagg
    36181 ccaaggcctt cgatgccatc ggcccataaa tcggcttcag gagaaaggcc gtagaaaaag
    36241 acccgtgctt tcgatttttc ttccattttg cgaacccagg gatcatcgaa gttgagaatg
    36301 gctacaccat ctgcgggcag tgcctgaatc aattcgcttt ttccgcgggc gatggcttcc
    36361 tgtgagcctg cacgttctgc atggaccgtg ccgacattcg tcaccacacc aacctgcggc
    36421 tgggcaatat cgcacaggaa ttgaatttcg ccggggacgt aaaaccccat ttcgagaacg
    36481 gcgcgttcgt accctgaacc aagtttcaaa atggtgagag gcaggccgat ttcgttattc
    36541 aaattgccgg ggttgcgcag ggtgcggtag cgagtgccaa gcacttctgc gatcgtttcc
    36601 ttggtggtgg atttgcccac actgccggtt acgccgacca cgcgcagatc caatttgcgg
    36661 cgccagaagc gggcaatctg ctgcaaggcg gagatggtat tgtccacgcg caggcagagg
    36721 ggagtggaaa gatcaagggg agattctgcc gttagaccgt tgcgcaggtc gagggtccga
    36781 aaagattcgg gcatttcctt ctgggttaaa gcaaaagagg cccccctctt gaatgcatcg
    36841 gcaatgaagt cgtgcccatc cattttttcg ccgggaatgg caacaaagag cgaagcaggg
    36901 atcacctgac gcgaatcgat ggctgcttcg gtgatgacag cattggcgtt cgcgatgcga
    36961 accgaggtaa tggcttcaag ggcgtcggcg agtgtgagca tgttatttga ctacaagttg
    37021 ttgacggatc gaatcgggcg gaatatccat aagcagaacg gttttttgag ccatttcagc
    37081 aaagactggg gcggcggttt cagaacccca aatggatgaa gaaggccgtt cgagccagac
    37141 atagatcatg aaacgggggt cgtcaatggg cccccagcca atgaaggagg cgttggtttg
    37201 ggtgctgtcg taaaacccgt tgacggggat ttgagcggtg ccggttttgc ctgccacccg
    37261 atacccgggt acaagagcca gtgagccttc agattcgagt gagacggcca gcattccaga
    37321 aagaatgtcg gcggtttgtt gtgaaatggg cgtacctgca aattgggctg gcacattgta
    37381 ttggcgtcca tcgcgcatca tggcgtagag tgcgtgaggt gtaacgacgc gcccgtggtt
    37441 ggcgatggcg gatgcagcca tcatcatttg gatgggggtg gcggtgaggc cctgaccgaa
    37501 ggaattggtg ccgagatcaa caggatacca gtccgcatca ccagggatct tgagcctgcc
    37561 cggtgcttcc ccggccagat ccacgccggt gagttgacca aggccaaagt tatccatgta
    37621 actgtaaaaa ttgccagcgc ccatttgtgt ggagagggtg gccatacaca cattgagcga
    37681 gtgctgtaaa caaccagtca tatcttgcac gccccagggt tcctggtccc agttttgaat
    37741 ggtggcgccg cctacaagga tggagcctgt atcgaggtag gtggtttcgg gggtgacagt
    37801 accggtatcg agcgcagccg ccattgtcaa aatcttgacg acagaaccgg gttcgtatgg
    37861 catggaaatg gcggggttga aatcgctggc gttgtcgtaa atggcgccgt aattccaaaa
    37921 ctcgttcaaa tccatacgcg gagtgacagc catggccagt aattccccgt tacgcggatc
    37981 catgatggcg atcgtgccgc cttgagcgcc atgttcaatc agggcacggt caaggatttc
    38041 ctcggcagcg gcctgcaggt cacggttaat ggtgagaacc agggtggtgc cgttgggaac
    38101 gcgcggaatt tcaaaagcct tgttggggtc ggtggggacg agtacctgca cagggttacc
    38161 agcgagcaga gtatcgtatt tttcttcgat gccgaaatag cccttgcctt cgcggtttac
    38221 aaagccaatg atattggagc caagggagaa ttccgggtaa ctgcgctgtg ggtgagattt
    38281 aaactgtaaa ccggtcaggc cgccgagcgt gccttccgga gcctgttcct gcaaggcctt
    38341 ctttaattcc tgtaatctta tggcctgtgc cgattcaacg taatctgcaa cgacgacgta
    38401 ggaaagaccg tcgggcgggt tgatgatcat atcgtagatg cggtcatagc tttcgccaag
    38461 ttcggaacta agcgcgaaag ctatggcatg cgggtctttg acggtgctca ggtccacgcc
    38521 gagttcgtac acagatttat tgcccgccag caaatgcccg ttgcggtcgt agatctcgcc
    38581 gcgatcagga tagaaggtgc gtaattcgta ggcatagttc gaggcctgct gtcggaatac
    38641 agcggcctcg gcgctattct ggatgcgcgt catttgcacg acgatggcaa gcgcgatcaa
    38701 ccccatgacg aaggtcaaca cctgcgcgcg gcgcgcatac tgctgtctca ttgggtgcct
    38761 cgcgaagcgg aggatgtgat gcggttatcc atccaatcga gcagggattg gttgtattcg
    38821 ggggggaagg tcaacgcgct taactgcggc aacggggcag aagagaggat atccggttcg
    38881 agggtgacat acccgggcac caccaaatac tccacttctt cggcttccat cgggcggaaa
    38941 ccaagctcaa gtgcgcgctg gcgcatgaca ctggcggagg tcaacgaagc aaggtgggtt
    39001 tgcaagtcgc cactcacctg ctggctgacg ataatcgaag aggtcaggtc ttggatttcg
    39061 cggccggcaa tggcagcctg cgaggtcaca tccagataca gggtggaaat catcgcaagc
    39121 gcaacgacca taagcagcgc attaccgatc cactggcgct gaacacgcca gggagcgatc
    39181 ttataggcat ggatatagtg gtttacgttt ggtatgttca ctgctcttct ctctctgact
    39241 acaacttttc aacaactcta agttttgcac tgcgcgacct cgggttatcc ttgatctctt
    39301 cgtccgaagc cacaatcggt ttcttattga tgatttttac aacagcctta cgttcttctt
    39361 cataaattct ttcgtaaggc ggattgacga tgtctttgct ctgctctcga aaatactctt
    39421 tgacgatgcg gtcttccagc gagtggaagg agatcaccgc gcatcttcca cctgacctga
    39481 gagctgccac ggcttgcgga agcgttttct ccactgcggc caattcgtcg ttgacggcga
    39541 tgcgcagggc ttgaaaggtc tgcgtggcgg ggtggacacg gtcaccgtga cggggagatg
    39601 ctttttcgat gacagcgacc agttcacggg tggtgcgaag cggtctgttc atcacaatgg
    39661 ctttggcgat cttgcgcgag tcgcgatctt caccgtagcg aaagatgata tcggccagtt
    39721 cccgttcatc gaaggtgttg acgatctcct ccgcgctcat cacagcgttg atgccgaagc
    39781 gcatatcgag cgggccatcc tgcatgaagg aaaatccgcg ttcggcatta tcgaattgca
    39841 tggacgatgc gccgaggtcg aggacgatac cgtcaatcga gtcccatttc aacgcggcca
    39901 gttgctggct gagggtaatg tgggaggctt gcacgagatg aatgcgtccc tcgtaaggag
    39961 ccaaggtctc acgagcgagc gccaaagcct gcgggtctac atcgagaccg agcaaaagcc
    40021 catctggcgt gcaagcctcc aggatcccgc gagcgtgacc gcccgcgccc aaagtgccat
    40081 ctacataccg cccgccgttg cggggttgca gagcgtgtat aatctcttgg taaagtacag
    40141 gtttatgctg cataactagt cgctggtcaa ctagttcgct ggtttcgaaa ggtcaagcgt
    40201 ggagaagcgg gcgttattgg cttcggtatc ctgcaattga gccatttgct cgccccagtg
    40261 tgcgggggtc cacacttcga agtattcacc ctgccccgcc acaaccagtt cgccgctttc
    40321 gattccaaga aaagcgcgca ggttttgcgg aacaaggata cgcccaacct tatccacttc
    40381 gatgggataa gcattcgaaa ggatcaaacg gcgcagtaaa cgcgcggttg ggtcggcaag
    40441 gttcatggct tcaatgcgtt cataaacctg tttgaagtag tcttcagtca tcaccataag
    40501 gcatttatca aagccttgtg taatgaaggc accaccagcc aacaactcac gaaagcgcgc
    40561 tgggatcatc aggcgccctt tgtcatcgag attgtgttgg aagtgaccta agaacatttg
    40621 tgctacaatt ccttttaatg cgacgaacgc cgggcaatcc ggcgtcctta ccactttcta
    40681 ccacttctta ccacattata ccgatttgtg gtcaaaaatc aagtggcggg aagcgttcgt
    40741 ttcattccat ttaaataaca atttggtaat gtccatgcaa aaacccccta ctttctccct
    40801 actcgaagcc tccaactggc gtgattatga attgctcgat tctggcgacg gtcaaaagct
    40861 ggaacgcttc ggcaaataca ttttttcgcg cccggaatcg caagccatgt ggagccgcgc
    40921 tctcccctct tcagagtgga acaacgctca tgccgttttc caacccacag ccgaagaaag
    40981 cggcggtcat tggatcgaaa aaaagaaact gcctgaaaaa tgggagatga aatatccgct
    41041 cccccatgtg caggatctga ccttttctgt gatgacaacc cccggccgtc acctcggcgt
    41101 atttccagaa gtcgcatccc attgggactt tatggctgat tccgtccgaa aggcaggtcg
    41161 gcctgcaaaa gtgctcaacc tatttggata caccgggctg gcatctttgg cggcggcggc
    41221 ggcaggtgcg caagtgactc atgtggatgc ctccaaaaaa tcggtgggct gggcacgcga
    41281 aaaccaggca ctctctggcc tgaacgaaaa accgatccgc tggatcgtgg aagatgcggt
    41341 caaatacgtc caacgtgaag aaaagcgcgg tgtgaaatat gatggcatca tcctcgaccc
    41401 gcccaaattc gggcgcggac cgaaaggcga ggtatgggaa gtgtacaaat ccctgcccaa
    41461 cttgctggaa atgtgccgcg cctgcctgag cgactcaccc ctcttcgtca ttgtcaccgt
    41521 ctatgccgta cgcgcatcgg cgatccacgt ggcgcaggca atggatgaga tgatgaaaaa
    41581 atataatggc aacatcgaca gcggcgaatt ggtcacccgc gagaaaagcg caaaccgcct
    41641 gctctcacag gcagtatatg caagatggtc gaaataaaaa aattccgagg ctcaacacct
    41701 cggaattttt ttggctttta tccgctgcta ctgccgccac ttgggctgct cttactgccg
    41761 cttgaacttg aagtgggaga cgtcaacttc ttcaacaggt cataccagaa ggatgatccc
    41821 tgcgaaaccg ccgcggcagt gacacccaat cccagcacct tcagaaaaat gaacaacgcc
    41881 caatccccta cattgttccc ttcggggaat tgctgagttt gccaccaacc gatcttgatc
    41941 gaatactggc tcaggtcatc gatcaggtca gaaagatcgg cgctggcgcc ttctcgtgcc
    42001 acaacagcat ttgcctgtgc agcagcaatg gctcgcaatt ccgcattcgt ccacaaatta
    42061 cgagcaagtt gaatggtatc ggtgccaaac agaatggtaa ttccgatcga aagaatgatc
    42121 acaaaactgc gggcacgggc acggaaggtg gcggccgcct gctctaaaat acctgtgaaa
    42181 tatgatgtag ccctcacccg taattctgca atgctggtca caccttgatt gatccaggta
    42241 ataaaggcct gcttgccttc agaatcgggc aatctgtcca ccaactcagc caattccaac
    42301 aactccatct cttttttggc ggtcaaccct gcgagatcaa agtacgcatc tacaagagtg
    42361 gcaactggga tcttctcgat catcttcggc tcggtcaccg acccaaacac actcaacggg
    42421 cccttaaacc gaatcgggcg caaagactga agctgcggca gattcttcaa atctccgatc
    42481 tttttatcac cgaccagctt gatcaggtat tgctccaaag ccctgccgcg cgattcgaaa
    42541 aattcgttga tccattgtgt gatcaatgaa accacagaac caagaatata gtatatgaca
    42601 atgagaccga cggctacttc gagaaccaat gaaatggaca tgtgtttctc tccttgggta
    42661 tgatgtttta cttcctgtca ggtactgtct tttccaactc cgcaagccat tccaacggcc
    42721 tgccccagcg tttacgctcc tgaacaccga cctgcttctt tcccggccta tcccacttgg
    42781 gaccgggcgg gcctgattct tcaccgagca tacttttcac accccacaag cctttgtatt
    42841 gtacccaatc tggctcagga ttcaagagca cccgccgcca ctctgcaacc tgtggctgtg
    42901 tcgtctggcg atccgacatt acccgcttga tatatctgct ggatcttata aaaccctcat
    42961 gtgaaccctg tccgctcaaa ccaatacggt gcccatcccc ggaggcgatc tccattggga
    43021 gggtcacaat ataatggtcg gtttcatcgc gcaatcgggc aaacatttcc tcggtcagga
    43081 attttccgcc ctgcttccaa aactcacgca acgcaatctc ccgcgccctt tggttcggat
    43141 tcaacaacaa aaacaggtaa tgaatgaaac catccagcca gaaaaggact tgctgcaatt
    43201 tcccgggctt gtacatccac ggtgaacgaa tgaccactgg cttgctgtaa tttgcatgtg
    43261 accccaaagc cacatacacc accggatgtg tcgtctcatg accatttgca tcaacagcct
    43321 tgcgtaccgt cttccatttt tcgatatttc ctgcaccatg ctgtgaaaac aaaactgcat
    43381 aaggttcatt attcttcaaa tacacagcca ccatctccca atccccttca tggtggttca
    43441 tcccatttgc agccaaccgc caatcattga atgcataaaa aaagtgatac tgcaggatcg
    43501 tccatgcaca accatcttca tctttttcat gcaatacccg tccgtaatat acaggctgcg
    43561 catccattgc aagcatcgcc gcatactcaa aatatgcctc acgtgcgatc ttctcagtgg
    43621 cttgtgaaaa catatccatc accacacctg gtccttcagg caccaccctg tcaagaatga
    43681 acttcaacgt acgcatcgaa agatagaaca agatcaccag atagatcggc agcaggacca
    43741 aatactccac tgcaacactc ataaccctgc ttggatgaag gaaaaaccag atcggtgtca
    43801 cctccgctgt aataaaagcc cccgccgcca atacagcagg gataatgcgc aaacgtatcg
    43861 gagaagccag cataaacaac accaatgccc ccagcaaaga agcaacaaac ataaactcca
    43921 cccccgcaaa attggcagta agccacccac ccaccatacc tgcagccgat agcaggcccc
    43981 accacaccca ggcatcggca tcatttagcg gcttattaat gaaacgcaga aagaattgtt
    44041 cactctgcaa tttcccaatc cgcgttatca tcggttcatg gatgtgcatc acgatctcca
    44101 acacaccctg tggcccgccg ggaaaaatat gacacttctc caaatacggc tccaccgcca
    44161 ttggaaaaaa acgctcgctc ttcgcaaaac gaagcacagg ctcatatttt tcaagaattt
    44221 caagttctgt cacatcgcct ctatggtata aaagcaaaca tgaacacaaa aaccctcgaa
    44281 caatccttcc taacccattt caacgccaaa cccgcattca tcgtacgggc acccgggcgt
    44341 gtcaacctga tcggcgaaca caccgattat aacgatggat tcgtcctgcc catggccatt
    44401 gaccgcgctg tctggatcgc gctatcaccc agaatggatt cacaggttca catccattct
    44461 ctggatatgg aaaccgcgct tcccaacgac ccgcaagctg tttcgacctt cgatctaaat
    44521 tctctcacaa aaggcaaagg ctgggcggaa tacatcaaag gtgttgccga ccaattgcaa
    44581 aaagcaggtt atcaactcca cggatttgac gcaatcatca caggcgatgt tccccgcggc
    44641 gccgggcttt cctcatccgc tgccgtggaa cttgccgctg cacgtgcttt tgccgtaaca
    44701 gcaggtttcg agtggaacgc cgcacaaatg gcggctctgg cacaaaaggc agaaaacgaa
    44761 tgggtcggtg tgaactgcgg catcatggac caaatggcaa gtgcggcttc acaggcgggc
    44821 cacgccttgt ttctcgattg tcgcacactt gaatatcaac tgcctcccct acccaaaggc
    44881 attgccgtgg tgattttaga tacttccaca aggcgaggcc tggtagattc agcctacaac
    44941 gagcggcgaa atcaatgtga agaggctgcg cgttggtttg gagtaaaagc cttgcgcgat
    45001 gtcagtgtgg ctgaattcaa cgaaaaaaca aaagacgcca gcgagttgaa tgtcaacgtg
    45061 ctcaaacgcg ccagacatat cgtcaccgaa aatgagcgcg tactggaagc tgttgaagcg
    45121 atgaaactgg gcaacatcca aagattgggc gagttattca atgcaagtca cagcagtctg
    45181 cgcgacgatt ttgaagtgac gaacgaagct ctcaaccaga tcgtcgcctg cgcccaggaa
    45241 caaccccatt gctttggcgc acgcatgacg ggtgcaggct ttggcggatg tgctgttgca
    45301 ctggtccgcg aggaaaatgc cgcagcgttc actcaggccg tttccgccgc ctacaaacaa
    45361 cggtcaggat tggaagcgag catttatgtc tgcaaagcca gtgatggcgc cggggtcatc
    45421 cagggataaa tagaataaaa aatggacacg gcttgcgtgt ccatttttta ttcttagcta
    45481 taagccatct gctcgacggc aatggggcgt ttgcgggtct gacgcttgat gatcttctca
    45541 gcagcgatgc gggtttggcg catctgtgcg ttgcggacga tcaacttcat gaatttcgta
    45601 tggttgatcg ggtcattgag aagtgaaaag tcagccgagc ctggttcgtg cggcgggtag
    45661 aaaatgccac agttggggtt gatctccagc atgtagatgt caccatttgc atccatacga
    45721 taatcacagc gggcgtatcc attaccaccc aattccttga agacacgcat ggtttgttcg
    45781 cgcaaggtct tttgaatctt cggctcatcc accaccgcta cagacatttt ttcataatcg
    45841 atccatttca tgtcgtagtg tttgaacgat tcaccattgg ggaaaatgaa ttcaaccggg
    45901 gtgaatgtga gcggcttctt gggatcatcg ggattttcag cgatcaaaca ggtgaattcg
    45961 cgtccttcaa tgaactcttc aatcaaagcg cggccgaatt catcaatttc aagtttaagt
    46021 tgttcccgca gctgctcgat cgtttcaacg cgggaattac ggcggatacc aacacttgca
    46081 tatccatgcg gcggtttgac gagcatggga aatttcaaac gctttgcaac cttctcggca
    46141 tcttcaacgc gatttacaaa aagccaattg ggggtgggaa ccgatacacg gcgagccgca
    46201 ttcttcattt catcacgcgt gggatcaaag aatttcgaat cggcacctgt aaaagcagcg
    46261 ttatgtttct caagggcgtt cacgagtgcg atgcctgaaa gagcatcatc gggtgtacca
    46321 tcacacagat tgataaaaac atcgacacct tcatccatca gatcgcggat ttgtttttca
    46381 acgttctttg gttccaccaa atgttgcttc cagcgatatc cgttcatgta tggtgatggg
    46441 tcatacggaa tatcgttttc atctggaaca cttgtcaata cgcagatata cataatattt
    46501 cctttgttgt tgtccttctt tttatttttg attcaattta tttgaatgta tgatttcaat
    46561 catcattcaa ttcattagca acacaattta atatgcaatc aatgaatcaa atttgttatt
    46621 tatatgttta ttgtatgcaa ctattgagtt atgtaaaggt ttttgaacat acaattatct
    46681 taaaatatca acactgatat atacatgcat tcatcatcaa aacatcaata aacatatgtc
    46741 gcatttaatt tctttaacgt ttttaagatt caagagcagg cgacacactg cctgctcttg
    46801 aaaaatagtc agaaattatt tttatatcac acatctcatc catgaaactg cggaaggtgc
    46861 tccttgtaag tcaacagata atcatcaagg atccgctttg caacatgcat atctgtaatc
    46921 accggatcca acagcagaca ttgcaatgcc ttttgataat caccctctac agccgcatca
    46981 acagaaagct gtgcaaccat taactcacgg cggcacaact ctgcaaccgc ttcaggtaac
    47041 gcaccgacat gcacgggatg aatccccgcc ccatccacat gaactggtgt ttccactgtt
    47101 gcccctaatg gcaggttcgt gatctgccca acattgggca agttcgctgc aagatgatag
    47161 tgattacctg cataagcaat attctcgatc atttccaatg caccttctga atctgtattc
    47221 aacaatcctt caatggcgac cgtcccattg gccatgtcat tcaagcgatc cagactgaca
    47281 tcacgtagat tggcgcgcac gtcccaatca tacaagcgga tattgaactt ctcccaaggt
    47341 ttggtgacag ggtcactgag ccatggaaga tattcgcaca aatgggtatc gccaggaaca
    47401 gggaataaat cgaaggcatc gaacacttca cgggtcagcg gctcaaaaga cgggtcaaaa
    47461 gccttgaaac gctctctgaa tagcggatac agatcctcac ctgtgcgttt atcatgcagg
    47521 gacaggatcc aggtaaagtg gtttgtcccg gcggcgcgta aatctacaag cggcagggtt
    47581 tggtggacaa catgatcccg caggtcatgc tccacgacat ccgcctgcat tcccgtgatc
    47641 ccttttggaa cttcaatacc aagttcttca gccagcacca tccccacaaa acaataacca
    47701 ataaatatct gatgacataa tccgacggct ttgatcttgc tatggcgatt caccaggtca
    47761 caaatacgca ccatcggatt cgtgaaattg ataaaccagg catcgggaca agcctgttcc
    47821 atatcacgga caatgttcaa gacaggcatt gcattgcggg cggtatgtgc gaaaccacca
    47881 gggccgccat tttctgcata aggttgtcgc acgccgtatt tgatcggaat atcgaaatcc
    47941 gccttccaca attcctcacg cggaccaact tcaatggcag aaacaacaaa atctgtacca
    48001 tccaatgcgt ccttgtgatt tgtgtgtgca gtgatgttaa aaccagaacg ccactctttg
    48061 ttcaggcgcg ctgcaagcct gtggacaata tcaaggctct gtgcattgcg atccacaaga
    48121 cgaagcgtgg agccattcaa ttttttcgaa cgcaacaacg cagaaagcgt gttttctcca
    48181 aacgatgcac ttcccgcacc aatgacagta atggtggtca tatattggtc tcctttatga
    48241 aggaagtata caggaaaaca cgaaaacaaa agaaaacagg caaaccagca gttgtgctat
    48301 tgcacatacc tgccaatcta cctgttcgct atttactgcg tactgtttaa gtttatcttc
    48361 aaactacgtc atcacccact tacttaatca tgatctccac ttcagcgctc ataccccaga
    48421 acatgccttc aggctgttca tccaatgaaa tggtgacggg gtatacaaca tctccaccaa
    48481 cagtttctga cacaggtgaa atgtgcagta ccttgcccgt tacggtgata tctaatgcct
    48541 cgatataaac atttacagcc tgaccgatct tcacgttcgg aatatcgcgt tcgctcagat
    48601 ctgtcgtggc aatctgcata tgggcgatgt cacccagcgt aattacagcc ttcccaacct
    48661 gtgcaagctc acctgcctga atattcacat caatgacggt ggcatcaaag ggtgccagca
    48721 gggatgtttg agccaaattg gctttggcag tttcaagttt ttttctggct tgttcaagct
    48781 ctgcttctgc ctgctgacgg cgctctggcg gcctatcgtg acgggcggga atccagtaag
    48841 tgaattcaag ttcggcggct ttcaattcca actctgcaga ggtgactgca agctcaaggt
    48901 cgggcgaagc aagtgtcatc aaggtttgac ccgctgaaac ggtatcgcct tctttaacaa
    48961 gcacttcctt cacaggcgcg gagatgacaa aactgatctg cgaatcctgt gcggggatga
    49021 cctcggcgga agcgaccact gcatcagatg agacaaaatc attttgtggc gaagcaggct
    49081 cctcggtctg aattggaggc ggggttgggg tggcaggcgt tccacaagcg gcgagaatca
    49141 aagtcgcagc gaggaacgtc atccaaatca tcttcttcat tctcgcctcg ttatttctct
    49201 tcgatcttca catcggcgct cataccccag cgcaaaccgt cgggttgttc atcaagttga
    49261 atggtgacct tgaaaacaac atccccaccg accgtttcag aagaacgcgc aatatcaaca
    49321 accttgccag tgaattcctg gtcaagcgcc tcaatgaaga cctgtgcggc ctgaccgatt
    49381 gaaacggagg gaatgtcctt ttcgctgaag tcggtggttt cgatctggaa gtgtgtgagg
    49441 tcgcccatca caatgatgat ctgcccgggg ctggcgaact cagcgggtga aatttcaaca
    49501 gaagcgatcg tgccatcgaa cggcgcaaca agggtggctt gtgaaagttg tgctttggcg
    49561 atctcaacag cagccatggc acggtcaata ttggcctgcg cggcatcgag tctttcctgt
    49621 gacgtgcctg tgcgtttgag atatgtcaat tgggttgtca ctgtgacgac attcgcttcg
    49681 gcttcagcca cccgtgcttc gaggatggcc gtatccaacg tgaccagagg ctggccagcg
    49741 gtaactgaat caccttcggc aacttcaaca gttttcacgg cgcccgaaag cgggaacgac
    49801 aactcaacct tggtcaccgg gacgacaacc gcagacgcgg tcgcgcttcc cccggaggat
    49861 tgcgatgggg catcaagtga aattgtggga acggcttcag gagtggcctg ccctccgcag
    49921 gcggtcaaag ccgtggcaag gattacaaat acgatcaata gcgtctttct cattttttat
    49981 atttctccaa gctggtgttt tgccatcgtt tttagatgac ggaaattttc ttgagttggt
    50041 tttatcacac cgagctaaaa ttcgatacgt ttttttcagg atgaactact atctttttct
    50101 atcaaaccat cgttcaaggt gacgattcgg tcggcatagc tgatgacacg cggatcatga
    50161 gtggcaaata caaaggtcac tcctgtttcc ttgttgagtt tcttcatgat atccatgacc
    50221 tgtttgccgt tggccgtatc cagattggcg gttggttcat cagccagaat aagtgttggc
    50281 ttgggggcaa gggcacgcgc aatggccacc ctttgctgtt ggccgccgct caattgatcg
    50341 ggacgattgt tggcgcgttc ggtcaggtcc acagccttga gcaaatccat gacgcgttcg
    50401 tggcgttctt ttggcgaatg tccatttagc aggaggggca tttccacgtt ttcatacgcg
    50461 gtgagggtgg ggatcagcgc gaagaactgg aaaataaaac cgatcgttcc acggcgcatg
    50521 tcggcacgtt gattgcggtt gagtgtggtg acatccttgc cgctgatcaa aacatgtccg
    50581 cttgaaggct ggtcaagaca accgatcatc tgcaaaagtg tggtctttcc cgaaccagaa
    50641 ggcccaacca acgcggtaaa ttctccactt ccaatggaaa ggttgacgcc gcgcagggcc
    50701 tgcgtttcca ctttcccaat tttatataca cgggtaacgt tctcaatttt tacgacttcc
    50761 atttttgctc ctatttttca gcacgcaggg cttcaaccgg ttgcatgcgg gaagccatga
    50821 tggcaggata caaccccgcc agtaaggtca ctacaaaggt catgatcgtc aggttgacaa
    50881 tgttctccac ggtcaacttg gcatagatcg tgtcggcgat cgccattcca ctcaacccca
    50941 tgtcaccaat ataaaatccg ttgacattga aatagagggt ggccagcaca ccgatgatca
    51001 atcccatcac cacgccgccg acagccagca tggaagattc ggccagaaac aacatcatga
    51061 tgcgacctcc tctcattcca attgccgaaa gaaccccgat ctcgcgtgtg cgttcgtaaa
    51121 cagacatgat caatgtatta atgatgaccg aagccgagat cgccagaatg atcatgtaaa
    51181 agatcgcgat gtaactttgg gcgaacgtct catactccac gaacagggca ttcaagtctt
    51241 tccaggtctt gatctccaaa tttgaagaaa cgctcaacgc agggacaacc gcatccgtca
    51301 tctctgtatc tttgagcagc acaaagatcg tactggcatg atcttccgtc cgcgtcattg
    51361 cctgagcttt tgccagcggt aaaaagaccg tggcactatc gaacgcataa gtatgggtgg
    51421 agtagattcc ctttacagta aaggtttggt cctgcacatt gccatcggcg gtgttgagtg
    51481 aaaggctcac actgtcgcct actttcaagt tcaacttatc tgccatcggc ttgccgatca
    51541 acaacacgtc gcggtcttca gggttgagat actccccgct gatgacccct tcgcggtacg
    51601 gatcgcttgc aggtgacaac gggtcaatcc cggtgatctt cgcacccgca gattgcgttc
    51661 ccgatgagag gaatccgctc gcgtacaaac gcggcgttgc agtctcgacc tggtccagcg
    51721 ctgcgatctt gcctgcgatt tcatcagggt tggccacaag gtcttcccat ttaaggcttg
    51781 acttgttttc gtcatacgtt gccgcacgga tctggatatg cccggtctgt aggttgatgg
    51841 ccgactcgat cgcactgccc atttcgcctg ccacaacaga cgacataagg atcaacagtg
    51901 caagcccgcc tcccaccgcc aatgcagaaa aaaaactacg gcggcggttg cggcccagat
    51961 cacggaaggc cattttaaga atttgcatca taaacttctc tctttcagtg gttgtggttc
    52021 aaaaccacct catacaaaat gcagcgcttc agcaggctct cgacgcccgg cttcagctgc
    52081 gggaatgagt gccgccaatg cagaaatgat caagatcatg gaaacccgca aaggcagttt
    52141 ctcaataccc caggttgggt aggcacggct ttggatcaag gccatgtaac tggcagcctg
    52201 agtcatattt ccaaaatcca gcccgacttt catcaaataa ccgttgatcg ccaatccgag
    52261 gataatccca gccgcaacac ccaccagccc gatcatgatg ccctcaagca cgaacagcat
    52321 ggatatttga ctcggcttga gacccattgc acccaacaca ccgatctcac gtgtgcgttc
    52381 gtacaccgcc atcaacaaca ggttgagaat acccacccca gcaatggcaa tgatgatgac
    52441 actgaaaaca tccatcacac cgccctttgt attgatcgca gaagccaggt cgggataatt
    52501 ggcttgaaac gattcgatct catagccggg caggctgggc tgcagcgctt cgatcacatc
    52561 gctttcctga cccagctttt caaggaagat cgccacttca gtggaacctg tcacctcgta
    52621 aagagcctgt gcttctccaa gagaaatgta aacactctgc ttttcgatgt cagccagtcc
    52681 caaatcaaaa ataccgacca ccgtcattgt gcgctgacgc atttgctcat gttgcgaacg
    52741 tcccaccatc gtgatacggt cgccaacctt cacgcccatc gcatcagcca gtcctttgcc
    52801 gatcagaaca ttatcgagat cagcactggt gagattgcgc ccctctttca cattctgccc
    52861 aatgatgttg actgtcaatt ccttttcagg ctcaatgcca atgatgccca ccgaaaatgc
    52921 accttcacgg ctggtcacca acccacccgt gctgatgcgc tgcgtcgccg ccaaggtcag
    52981 cggatttgcc tccgcggcat caatcaccgc ttgcggatca gtcagcggaa aaagcgggtt
    53041 tgaattcgcc tcagcccggt acccttcagc atgcacttga acgttgcccc ccaatatttt
    53101 cacggcattg ccgtagatcg catcggtaaa accattcatc agcccgtcgt accacatcat
    53161 caaagccagc gtcaaagaca ttgccagaac aatgatgacc gtgcggcgct tatgccgcca
    53221 gatgttgcgc catgcaaggc gcagataaag ttgcattctt actccttttc tctcgaaaat
    53281 tcgagagtcg tttttcaaaa taattacggc ttgggcagcc attctttcat cgtctcaata
    53341 tcacccaaag gcaaagaacc tggcggcgcg ccgaggatcc gctccacaga atcggaatac
    53401 gcgccgatca aagcctcaag gcgttttaca gcatctggag gcggatgatc cgtcaacagc
    53461 aattcgacaa acttgtcctc aaacccgtgc gataacccca caaagatatt cccaaattgt
    53521 tcggggtatc ttgtggaaaa cacaccttca tcaaccccct gttggatgat cgtcgtcagc
    53581 atcggcccga tccgttcaat ggatgcagcc tcctgtttct gacgcatgat ggcattcgca
    53641 tctgcccgcc acacccgaaa cagatcaagc atgaacgcct tttgcgccac cttccaccgc
    53701 cccgccgtat caaaataacg acgcatcttt tcaatggcag ataaatcctt cgactccacg
    53761 atcgggcgca acaccatctc ggcttcatcc agcatccgct cgatcaaacc atccaacaac
    53821 gactgctttg aatcaaagta atgataaaac gcccccttcg agatattcaa cgcatccaga
    53881 atattctgaa tggacatttg ctcatacccc ttggtgtaaa ccaactgacg agccacatcc
    53941 aggatctcat tgcgccttgc tacataatct tcttctttaa caactctggg catagcacct
    54001 tctttataaa ccgaccgtcg gtttattttt cgagattata tatcgggtga atgcctgtgt
    54061 caataggatt cttaacgata aaaaagcgcc tctcccttcg ccgaaacctg cttctatttt
    54121 ctcgaacagt ttctccccga aaacaaaaag annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    54241 nnnnnnnnnn ncaccaatcc catcttcccc atcaccatct tcaccatcaa ccccgcccgc
    54301 caaaaagcga cagccgccat cccatgcgcc agcactgcaa acaacggtgg tttttgaaat
    54361 gtgtaatacg tccaacactc acgggtggtg ccccacaact caagaaaata accaagccct
    54421 gcccccgcaa tgaaagtcaa caaggccagg cggtgatcgg tcggtgtcag gatcagcaaa
    54481 atgcacaaga tcgtcgcgag ccatgtgtag gatttgtcga atgtcggaga aacaaagacc
    54541 agcatcaagg ttaagaaaga ggcgaaaatg atccagtaga gaatgctgaa tgcggaatga
    54601 tgaatgctgc gtttgaagcg aatttggaat cgctcaatca tccaacttaa gagtctggtg
    54661 attcgatcta tcgagaggct tgcaatcggc catgcgggga taatccataa aggcgggcgt
    54721 tcggcggtgt agtaatgcca gagattcgtt tgtgtgcccc atgattcaat cgccagcccg
    54781 ccgcaaatgc ccacgaaaac gatcaacaca tcggtcttga gattcgcacg cgccacgatc
    54841 gtaatggaca tgaacaggaa tacacccagc aacagccagt ccatgtataa ccaccacggg
    54901 ccgttccaat caatataaga gagcacttct tctgcaagcg gccaccaaat gtacacgatc
    54961 aaaaagatcg taacaaaaaa tccgccaagc aaaatggatg agtcacgggt ccagaagggt
    55021 gtttttttat tttccatgcg cggattatat tgtacgactc aaagaataag atatatctta
    55081 tgtactattt ttttaatctc attcgagata acacacgtta taatgaagcc attgattagc
    55141 aggttgtaat caaaattttc catcacacac ttgaaaggaa aggattacga acatgagttt
    55201 cttcagcacc attctacaaa agctcggcct caaaaaagag gaacccgccg caacaccccc
    55261 tgctgcggct cccgcacaaa ccacttcaac ccctgcccct gcaaaaccac ctgtaggata
    55321 cgggccaaca accactgcat ccaccgctgc cgcacccaaa cccgcaggcg ctggcacctt
    55381 gtctgaccgc cgcgtacagg atgatatggc cgtcaatcac gaaccgatga agcccgctgt
    55441 caaagccatc tctgaagtag atgtggtcaa gcatttggaa agcctcggtg caggttcagg
    55501 cctcgactgg aaagtctcca tcgtggacct gctcaaactc ctcgatatcg acagcagccg
    55561 cgaagcccgc aacgaactcg ccaaggaact cggctgcccc gaagatttga tgcacgactc
    55621 tgccaagatg aacgtgtggc tgcacaagac cgtcctcaag aagatcgcag aaaacggcgg
    55681 caatatcccc gccagcatgc tcgactaatt tcacaccaat ttataaaaac agcgcggatt
    55741 aatttccgcg ctgtttttat ttaagtacgg caagatttat cttgccaatg nnnnnnnnnn
    55801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    55861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cggaatcccc aaggcctgaa tcaaccgcgc
    55921 ccaaaaattg acctgcgccg ccgtgctggc caggatcaca aactcacgct cagaaaaagc
    55981 tgcttttact ttatccacca tttcagggtg gaactttaaa ggcgtggcag agatcaggcg
    56041 ggtgtaggca atcgccagcc tttcgcgctc cgaaaaactc gctactgatt cataatctcc
    56101 gcgcaaagac tcggcttctt catcggtgat tccgtgcttt cgatattcgt atgaattcat
    56161 atccacgcaa aatggacaag ccactgcata cgatgccgcc atgcggatca atttcaacat
    56221 gcgctgattc atgcgcccat cttcgtgagc cacaagcgct tccatgatgc ccgacccaat
    56281 ggccgccttc ggataccagg ccaaaatccg cggcggaagc atgatccgcc cggtgatctt
    56341 ttcagagata taaatcccca gtttcaaaag caggggaatt tgaggaggtt ggggaataaa
    56401 agtcataacc agattataag cctgtgtaat ttgcaaccgc ggattcgcaa ctctcgcctt
    56461 ccattttgcc agaaatggga gagccagaca aaccgctttc attggcaact gctcataact
    56521 atgttaaact gaaaatgctt tcattctgct ttcagatttc tttcactcat ggcctctcta
    56581 aaaaagtccc ccaccatcta cgatgtcgcc gaagtctccg gcgtaagcat ctcaaccatt
    56641 tcgcgggttc taaattcgcc ggacaaggta aaccccaaga cccgccaaca ggtaatggac
    56701 gccatcgaca aacttgggtt cgtccccaaa gccgaagcgc gtgcccgcgc catgcgcaac
    56761 accaaccgca tcggtgtgct gaccccgttc ttcaccgccc catccttcgt gcagcgtctg
    56821 cgcggaattg ccggggcgct ctcaaaagaa aactacgaat tggtggtgta cacagttgaa
    56881 tctgcggccc aactggaaaa ctacttcgct tccatcccac ttaccagcaa tctcgatggg
    56941 ctcatcatca tgtccctgcc catcagcgaa gccaatgccc gccgcctgac ccagtacgat
    57001 atccccaccg tcttcatcga gtacccacac cccaccttga gcagtgtcga aattgatgat
    57061 gtagcaggcg gacgcatggc cgcagaatac ctcatcagca agggacatcg ccgcatcgcc
    57121 ttcctcggcg ataccgacct gcctgaatac gcgatccacc ctgtcagttt acgcttatcc
    57181 gggtttcggc aaacccttca aaatgaaggc atcgaaatcc ccgatgaatt tgtccgtctt
    57241 gcgccataca cccaggaaca aacgctgcgc gtggccaatg aactgctcaa tctgcccaat
    57301 ccgccaaccg ccatctttgc cgccacagac tttcaagccc ttggtgtgct caaagccgca
    57361 cgccagatga aagtgcaagt gcccgaacaa cttgccattg tcggcttcga tgaccttgac
    57421 ctcgccgatt acgctgacct caccaccatt cgtcagcacc ttgatgaatc aggccgcctt
    57481 gcggttgaaa ttctgctttc ccgcatttcc gacccaaccc gccccgcgca acacattcac
    57541 ctacccctca ccattattga acgccaaacc gcataggagg cactcatgcc catccacacc
    57601 tcaaccaact attgggttct tgaaacacgc cacacggcct actcctttgg cctgaacaac
    57661 gccaaattgc tcacccactg ctactggggc acacgcctgc cttttattca agattatccc
    57721 gcaccattca accctgttca ctgggcctcc accgatggct tcgaccattt tgtccctgaa
    57781 gaatatcccg gttacgcagg caccaaatac accgacccct gcctcaagat cacctttgcc
    57841 gatgaagtgc gggatgtggt tctcgaatat caggattttg agatcagcgc atctcccata
    57901 ccgacacttg ccctgcgatt aaaagacagt ttctacccgt tgcaattgac tctgcactac
    57961 cgcatccacg aagaatatga cctcatcgaa cgctgggtca ccttcgaaaa cctcggcgat
    58021 caaccgatgc tcatcgaacg cgccttctcc gcaaagtgga ccctgccgcc tggagacggc
    58081 tactccctca cccacatgta tggccgccat gtcggcgaat tcaacctgca acgcgaagaa
    58141 ctcacccccg gcctcaaagt catcgaaagc agacgcatca ttccaagcca ccgtgccaat
    58201 ccctggtttg cagtagatcg aaatgccagc gaagaccacg gcgaggtctg gtttggcaca
    58261 ctcgcctgga gcggcaactt caaaaccaca gcagaaatga ccgaattcgc ctccacccgc
    58321 atcagcatcg gactcaacga ttgggatttc gcatggaaac tcatgcccgc acaatctttc
    58381 agcacacccg cttcgatcag cggctacacc caaaacggat tcggcggtgc aagccgcctc
    58441 ttccacgatt acgtccgcaa cgaaatcatc ccgcacggaa aggttccgca caagatcctt
    58501 tacaactcgt gggaagccac cctcttcaac gtggacgaaa gctcgcaaac cgcactggca
    58561 gagatcgccg cgcaaatggg cattgaacta ttcgtcatgg atgatggctg gttccacaac
    58621 cgcaactggg ataacgccgg tctcggtgac tggttccccg ataaggttaa attccccaat
    58681 gggttgagcg gtttgatcaa aaaggttaac gacctcggca tggaatttgg tctgtgggtc
    58741 gaacctgaaa tggtcaaccc cgatagcgac ctctaccgca cccacccgga atgggtcatc
    58801 cacttcccca cccgtgcacg caccgaaagc cgcaatcaac tcatcctcaa catggcacgc
    58861 gaagatgtgc agaattacat catcaccaca ctcgataccc tgctccgcga aaacaacatc
    58921 gccttcatca aatgggacat gaatcgcggc gtgagcgaac cgggctggcc gcaagccccc
    58981 ggcgaccaac gcgaactttg ggtgcgttac gtgcagggct tgtatcatgt ctggggcaca
    59041 ctgcgtcaga ggcaccccaa catcatctgg caaagctgtg ccagcggcgg tggacgcgcc
    59101 gatttcggca tccttcaatt tgcagaccag gtttgggtca gcgacaacac agaagccacc
    59161 tcgcgcttga acattcagga aggctactca caatttctac cagccaacac aatggaagcc
    59221 tgggttaccg attggggcag ggaaatcgtc ccgctcgaat ttcgtttcca cgtcagcatg
    59281 tgcggctctc tcggcgtagg cggaaacctc cttgaatggg atcaggccca tcgtgagtcc
    59341 gcagcgggtt atatccgctt gtataaagag attcggcaca tcatccaatt tggcgaccag
    59401 tttcgtttga tctcacctca aaacaacgat tactcggctg tgcaatatct gagcaaagac
    59461 aaaaccgaag gcgtattgtt caccttccgc actcacatcc ctgacccatt caacattccc
    59521 atgatccatc tgcgcggcct tgagccagaa gccttgtatt ccattgaagg ctttgaacaa
    59581 ccccgctccg gacaggcatg gatgcaggca ggcttgaaag tggaattgaa gaatctacag
    59641 agcaaaattt tgcggattca acgcctcacc ccaaactgat aactgctcgc acacctcaaa
    59701 cattttctcc ccgaaaaaga aaatttccct cttgactctt gcaaatgaaa gtgctaaact
    59761 gtcacttgaa agtgctttca agataacact ttcagacaac cttctcaaac gttttaggag
    59821 gtgcgtgcga tccccctggc cctgcccttc acaaacgcaa tcttatttcc aaccaaattg
    59881 aaagaggaga acaatgaaca agaaatccct gttatggtct gtgatgagcc tgctcgttct
    59941 ggcttccatg ctgctttcag cctgcggcgc ccccgctact gaagctcccg ccgaagaacc
    60001 cgctgccact gaagccgccg aggctcctgc cgccaccgaa gcccccgc
//
LOCUS       DAOQLC010000008        56018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_128_length_56018_cov_24.161750, whole genome shotgun sequence.
ACCESSION   DAOQLC010000008 DAOQLC010000000
VERSION     DAOQLC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 56018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128_length_56018_cov_24.161750"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..>1184
                     /locus_tag="PL000_02770"
     CDS             109..>1184
                     /locus_tag="PL000_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC87847.1"
                     /translation="MNNIATQTIDLAIQIQQIPAPTFSEGQRAEFTRDLFLKENLKDV
                     SMDSLGNVYARLPGKQKKAKPLIVSAHLDTVFPTSISLQVKKEAGRIVAPGIGDNSLG
                     VAALFGILWSLRERNISLQHDLWFVANVGEEGLGDLRGMRAVVERFGANVIGYLVLEG
                     LALGHVYHRAIGVRRYRISVKTAGGHSWSDYGKPSAVHELANLVTQFAGIRLPREPRT
                     TLNVGTIHGGTGINVLASESKCELDLRSEDPKTLLKLIHQVEDLLKDSDRPGVRVLAE
                     IIGERPAGEIPADHPLVKLAVDCTREQGLESALTSGSTDANIPLSSGIPSVVMGITTG
                     GSAHTPQEFMEVDPVEKGMEALVR"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1702..3411)
                     /locus_tag="PL000_02775"
     CDS             complement(1702..3411)
                     /locus_tag="PL000_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC87848.1"
                     /translation="MNEQTRPTTVPDQLQTLSLQVGKIRIDLEESRLKLPKNTGEDLR
                     HIETSLMLISEKIDAFQREHNNMLALANIGQVVNSSLELDEVLRIVMDNIVRLTKAER
                     GFLMLRDDKGEMVTRMARNWEMESIHSSELTVSRSVVGRVIETGEPIITTNAQEDQRF
                     VGQESIVAFNLRSILCVPLKVKNDLIGVIYADNRIRAGIFADSERNLLVAFANQAAVA
                     IENARLFSSLKNTLEEVTGLKNLMDNIFASIASGVITADIQNQITLTNRAAERILGSS
                     SSDIIGHHLNETLASVSSELEPHLTEVRQTDKPIVDLEISHHLPNHGNVDWRFNLSPL
                     KDAGQKTQGIAIVLDDLTERKKLEAQRRLFERMVSPAVIEQLDPNSLQLGGKRTNITT
                     LFADIRGFTSFSENQEPEKLVQILNRYLAAMAEAVLVQAGTIDKFMGDAIMAWFNAPI
                     PQPDHTLRAVKTALIIRESVENLYKELPPEFHLGFGAGIHCGDAVLGLIGTEKRLEYT
                     AISDAVNTAKRIQENSARNQILISASAYERVSKEINARPHADMSVKGKTQPLGVFEVL
                     GLK"
     gene            complement(3418..5802)
                     /locus_tag="PL000_02780"
     CDS             complement(3418..5802)
                     /locus_tag="PL000_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNC87849.1"
                     /translation="MEKSSITNSNAESSARTSVWDWRAILLTIALIEISSSRLVITEW
                     VPYLYFTQTISLAGVILGFALGYSIFSRKAATRIAVGYSLILLPAQLLNAVERTDWVW
                     PDLVVLFNRLFTSMAQFIKGQPVYDQLFFISLVTAGYWIIGISAGYWLTRHRDFLNVI
                     LPSCLAMLVVQIFDPTESRRMWALAFYLFAALLLLGRLYFLENQTYWRKTRFLLTAET
                     INNLERGALTVAAITVFVAWSIPGWIKQIKPAAKAWREISEPILERLSDAVSALESPY
                     AGSNSGGEFYSSELSLGQQAAEGDTPVFFVTVKESEFVPIRNYWKGRMYDLYINGRWT
                     TLSDSNTPFVPDTDQLEVEYPENRFEMEFTFTNNLTSQNLLYGPSETIWVSREGTVTA
                     TEIGSSLKDVTAWNATPGVSSGGRYTVRALIADPTQEDLRAAGTDYPEWVTERYLQVP
                     ENIAPALRTLAEEITAPYDNPFDKTQAITAYLRNEIEYTTEITAEPPENRDPVLWVLF
                     DYKKGFCMYYASAEALMLRSIGIPARMAVGFAEGTFNEIDERYVVTFEDAHAWPEVYF
                     PGIGWVEFEPTGNQLPIERAATRDVINPDATPDPVTAETPAANPLVPPLLEEPPNLTT
                     QQNSGATQADRIELYKTIALYSLLTMTVVFVVFAFRRYSLDDRLPVYLASRYTKSGNQ
                     PPRWLNRWLRWTTLSPIERTFQAVNLSLYLLGRPQPRHITSQERAKALIERMPSAEPQ
                     VLALLQEYQSTIYTPKMGSLTTARKAAALILLKTWQIRAKEALQSFDYRYNQLR"
     gene            5874..6872
                     /locus_tag="PL000_02785"
     CDS             5874..6872
                     /locus_tag="PL000_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC87850.1"
                     /translation="MQTLIEGGIALIIAIQSLGDWLIAPMKFFSYLGNEEFFLLVLPL
                     LYWSVDSALGLRVAFILVTSNLFNHVGKLLFAGPRPYWVSSDVRALWNEISFGIPSGH
                     AQIAASVWGMFAAYSKKNWVWVGSITVIFLIGFSRLFLGAHFLHDVLLGWLIGAAILW
                     AFVRFWDSAAAWLEKKSMGGQIMVGFIVSLIFIAIGFGASLFRSGFQVPETWIANAAL
                     AGTEVPAPLDPNSTFTSAGTLFGVVIGAAWIHSFGGYQASGPVAKRAIRYVIGLIGIL
                     ILWMGLGAIFPRGEGFIFYTLRFIRYTLIGWWVAGGGPWLFVRLKLASWNTGENSI"
     gene            6914..9163
                     /locus_tag="PL000_02790"
     CDS             6914..9163
                     /locus_tag="PL000_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87851.1"
                     /translation="MTLEKNTLLHNRYRIIEILGQGGMGSVYRAIDENLGVAVALKEN
                     LFTTEEYTRQFRLEAVILANLRHPNLPRVSDHFELGEQGQYLVMDFIEGEDLRFRMER
                     LGTITEEDAVQIGAAMCDALAYLHSRKPPILHRDIKPGNVKITPDGHIFLVDFGLAKV
                     YQGASQATTTGARAMTPGYSPPEQYGTARTDPRTDYYSLGATLYAALCGVIPEDGLAR
                     AMDNAQLTPLRKRNPKISRRFAAAIEKAMAIDPSDRFQNAEDFKKALLGSSSKSQSRP
                     GGYTVTPPPAEAFLPNDPSEVVAAKEKEKTPPRQMTGPVQPASAEEEQPFVSPLKKQK
                     ERERKRRNALMRFMAVLILLAVIGVLVFAPGVIPADLRAQIPFLAPTSTATSSPTSTA
                     LPTFTLTSTSTATDVPPTATRTKVPPTHTNTPPPTGNIPVEATLTNTPSVTSTPTLVQ
                     PSPTPSLSVTPIGGGSGQIAYASNRTGLPQIYLADIASQTATQITEMPDGACQPSWSP
                     DGQRLVFISPCKGMDEIYYQAALYIINVDGTDLTPIATVPGGDFDPAWSPDGKSILFT
                     SLRTGQMEIFMVKLDDLSNAIEVTKGAKSVESRQPSWSPDGKQIVYSVKRFGVYQIWL
                     MNADGTEPKQIVRSGVAFSDYLPTWSPDGSLILFNQRCATSFCLPYLMSISSTDRSVE
                     QGTLLQLGVTSVEDVHYSPDGFHLLFEGEEAGENNDIIYVTVTGANRVRITTDPGLDF
                     DPVWRPTPK"
     gene            9412..11526
                     /locus_tag="PL000_02795"
     CDS             9412..11526
                     /locus_tag="PL000_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87852.1"
                     /translation="MILENETLLHNRYRILENLGQGGMGSVYRAVDENLSVDVAVKEN
                     LFTTEEYARQFRLEAVILANIRHPNLPRVSDHFVIAGEGQYLVMDFIEGEDLRQRMER
                     LGALPEDEVILVGAAICDALTYLHARKPPILHRDIKPGNVKISPDGHIFLVDFGLAKI
                     MQGNQATTTGARAMTPGYSPPEQYGTARTDARTDIYSLGATLYAALTGVIPEDGLARA
                     MDNAQLTPLRKRNPDVARRLAAAIEKAMEVDPADRFQTAEEFKLAMLNAKSRTQQLLG
                     TYTIAPPPSEAERKSDPVKAPNIIKHSSAALEDQEFVSPRKKLLERNRRNMRALVGGL
                     LAVGFVVFLGIMLFVPNAMPESVRRLFPFLPTTEPTTIPTEIASIPPTPVAVVVDEPT
                     ATLVVSTPVQVTPTSSVNQAVEPTPIGADYLQIAYVSTRTGLPQIYLTNILTDQSIAV
                     TNMTGGACQPAWAPDGSKLVFISPCAVKKDTYPEASLFIINSDGTGLQPLPTALGGDF
                     EPAWSPDGKSIAFTSLRDGYMQIYSYDIENDKIQRLVETDATTAARQPAWSPDGRQLV
                     FSLKRVATYELWLMSSLGANERLLFTSGNVFSNQYPVWTPDGKFILFSQRRPEEATFP
                     NLFSHKIGDNNDAALKLALSVRSVEDVDYSPGSFWIAYESGGDRGYHVYYSTPAGGNL
                     TRVTEDVTFDDFDPAWRPISTP"
     assembly_gap    11552..11651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11720..12757
                     /gene="hrcA"
                     /locus_tag="PL000_02800"
     CDS             11720..12757
                     /gene="hrcA"
                     /locus_tag="PL000_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNC87853.1"
                     /translation="MPELTERQRTILQLLIRDYIETAQPVGSKRLVERFHLDLSPATV
                     RNEMSALTEMGYLRQPHTSAGRVPSEDGYRAFVGEMIHKAELPASVQNTISHQFFQAR
                     PDMDQWMTLAASILANQSQGVSVVTAPHAETIRFKHIELISTQGRQVLMVLVMDGGEV
                     NQQILTLAEIVTQERLSQAAAHLNSLLAGLSMDSIAAVSSRADALEQDIITILLQDMK
                     RTSESVSGEIYTDGLTNVLDEPEFAESDEARWALRLFEERSTLQGLLARASSNSSVGG
                     LQVLIGGSGGWDELRQCSMVVARYGVPGLLTGTLGVLGPMRMSYARTIPTVRYVAGLL
                     SDLVNDTISGE"
     gene            12825..13370
                     /gene="grpE"
                     /locus_tag="PL000_02805"
     CDS             12825..13370
                     /gene="grpE"
                     /locus_tag="PL000_02805"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNC87854.1"
                     /translation="MADEKDKQEEIDETQPSAEEMPVENGQASAEREALINQLKEAEA
                     RVIEYKDGWARSQAEFQNYKKRIERDNELTYASMKGDIIKKVLPALDDLERALQNRPA
                     DDAWASGIELIARKMQNILEGEGLKRIDAKGKPFDPNFHEAISHEPADGVESEHVIDV
                     VQNGYMLGDRVIRPALVRVAQ"
     gene            13455..15335
                     /gene="dnaK"
                     /locus_tag="PL000_02810"
     CDS             13455..15335
                     /gene="dnaK"
                     /locus_tag="PL000_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC87855.1"
                     /translation="MAKIIGIDLGTTNSVAAVMSGGEPIVIPSAEGERLVPSVVAMNK
                     NSERLVGRVARNQAITNPQNTIFSVKRFMGRKSDDPEVERTRKRVPYAVNAADNGDVR
                     VRLGERDYSAPEVSAMILAKIKADAEAYLGETITQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKKNEVIVVYDLGGGTFDVSILDVGDGVFQVRATSG
                     DTFLGGDDFDLRIMDYLIEQFKKDNGIDLNNDRQALQRLKEASEKAKIELSTVMQTEI
                     NLPYLTADANGPKHLVMTLSRAKLEQITADLVDRTIAPVKQALSDAGLNAGDINEVVL
                     VGGMTRMPAIQDRVRAFFGKDPHKGVNPDEVVAIGAAIQAGVLGGEVKDILLLDVTPL
                     TLSIETLGGVATPQIERNTTIPTRRTQVFSTAGDNQTQVEIHVLQGERPMANDNKSLG
                     KFILDGIPPAPRGVPQIEVTFDVDANGILKVSAKDKATGRSQHITITASSGLSDSEVE
                     KMRKDAEAHAEEDRKRKDLIEARNNADNTAYAAEKALREFGDKIPADVRADVEAKAAE
                     VKSAAQGEDLEKIKAATEALGLSIQKIGASVYEQGQAAGGGEAGESSSAGPDVVDGEV
                     KE"
     gene            15412..16539
                     /gene="dnaJ"
                     /locus_tag="PL000_02815"
     CDS             15412..16539
                     /gene="dnaJ"
                     /locus_tag="PL000_02815"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNC87856.1"
                     /translation="MSNQDYYETLGVGRNASDDEVKAAFRKLARQYHPDVNKEPDAEE
                     KFKEINEAYGVLSDSDKRARYDRFGKEGLGGMGSGGFHDYSADFGDIFEDLLSGFGFS
                     SGRRSRNSTRPRRGRDLQMAVSLTFEEAVFGVDKDIEFEREETCSRCNGNGAEPGTSP
                     VKCSTCNGQGEVRQVRQTFLGQMVQTAACPTCNGRGETISSPCKTCRGGGLERKHIKK
                     KVQIPAGVDHGTQIRLNGEGAPGVYGGPSGSLFLVLDVKQHQFFKRRDNDVVLNLDIN
                     VAQAVLGAEVDVPTLDGEEKLKIPAGTQPGKVITMKGKGVPYLRRKDRGNQLVIVNVA
                     IPTKLTKEQHQLFEKLAESLGTSVKPQEKGFLDWLNEALGG"
     gene            16722..17147
                     /locus_tag="PL000_02820"
     CDS             16722..17147
                     /locus_tag="PL000_02820"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNC87857.1"
                     /translation="MDFSSINWLAVVVCVLASMMIGGLWFGPKTFFPIWWKAVGKADV
                     QAPGSGTSMAIVWGLIVVSSFVQAMFMALMVNAMGSLSGGATLGSGALTGFLLWLGFV
                     APSSLTNKLFADRIKAWMIEVGNHLVTFVVMGAILGAWH"
     gene            17385..17837
                     /locus_tag="PL000_02825"
     CDS             17385..17837
                     /locus_tag="PL000_02825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87858.1"
                     /translation="MPNTIRPYKTEDFEAVTRLWFDAQTVAMPEMMARMGYEFSEAQE
                     YFRNIVVNENQIWVYERDGEPLGFLAIQGELIDRLYVHPAWHRQGIGQALLNKARELL
                     PKHIWLYTHTANRMARAFYEKNDFIAEGFGSSPPPESEPDVEYHWRAK"
     gene            17864..18979
                     /gene="alr"
                     /locus_tag="PL000_02830"
     CDS             17864..18979
                     /gene="alr"
                     /locus_tag="PL000_02830"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC87859.1"
                     /translation="MRPTFLEVNLTQLKQNLENIRAKVAPAKVLVVLKANAYGHGVDG
                     VAPFIAPFADYIGVAIAEEGIHLRKMGIATPILVMGGTLPQQLPDFFEYDLTLAASSL
                     DLLTAAEQLAASTRKRLKIHLKVDTGMERIGVREYEADSLIELALSCSHLDLEGIFTH
                     FANSEVMSLQKNMTPLSLQLERFEEILALFEKRGAPQPRIRHTANSGAILLHPESYYD
                     MVRPGVLFYGVYPGRDVEKTIEVRPALTWRSKVVYSKITQPGRGVSYGSLWQAEAESR
                     VVTVPCGYADGYFRRMTNQARVNINGRLYPQVGRICMDQFMVNAGEDVVKVGDEVILL
                     GGGITAEDLADWTGTNEYEVMTNISARVPRVFTRAVQ"
     gene            19008..19613
                     /gene="lspA"
                     /locus_tag="PL000_02835"
     CDS             19008..19613
                     /gene="lspA"
                     /locus_tag="PL000_02835"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC87860.1"
                     /translation="MNRVVKDYLVLFGVGGTSIALDQWTKWLVRTNIEFGMQWLPDSL
                     MWLSPYARIVHWYNKGAAFGMFQNGNIVFTILAFIVIGAIIYYYPRVEAGDWTLRLAM
                     GLQLGGAAGNLIDRLRVGQVTDFISVGTFPVFNIADASISVGVAVLLLGVWLNERKEK
                     LNAAQQSTPEGEQPAAAVLNGVGPVDGVAGGEQPKSNGGQS"
     gene            19610..20530
                     /locus_tag="PL000_02840"
     CDS             19610..20530
                     /locus_tag="PL000_02840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC87861.1"
                     /translation="MSDRVEKFVYDGEKADRLDKFLVTCLPEFSRARLQGLIDEGFVT
                     INNVRAKKSGQSIDPGAEIEVRIPPPVPSGLVGEDIPLDIIFENDDLLIVNKPAGMVV
                     HPAAGHDSGTLVHAVLGYDPDLEGIGGEERPGLVHRLDKETSGLIVLAKNERAHRWLQ
                     DQFRLRTVEKTYLALVDGMPPTPTGRVEASIGRDPSHRKKMAIVTAGKGREAVSEYKT
                     LEAFKNHTLLEFHPHTGRTHQIRLHCQFLGCPIVGDSIYGRKNPTVDLDRHFLHAYRL
                     KIVLPGEKEPRVFTAALPEELEGVLTDLRG"
     gene            20611..21651
                     /gene="ltaE"
                     /locus_tag="PL000_02845"
     CDS             20611..21651
                     /gene="ltaE"
                     /locus_tag="PL000_02845"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HNC87862.1"
                     /translation="MEFVDLRSDTVTKPTPEMREAMAEAEVGDDVYMDDPTVNALQEK
                     AAEMLGKEDALFVPSGTMGNLLALLVHCQRGDEVIVGDKSHIYLNEAGGMSALGGIHP
                     RPLQNQADGTLLLDEIRASIQTEDVHHTITRLICLENTQNVCGGVVLSAEYLQAVGKI
                     AQENGLAFHIDGARIFNAAAALNISVKDLVAPADSVMFCLSKGLVAPVGSMLVGGRKF
                     INRARHLRKMLGGGMRQAGVLAAAGMISLEKMSTRLGQDHARAKKLFEGLQQVRGLKL
                     DAFGPSSTNMVYFDLADEVKLSVNQVVEEMKKHGVLVDWAGPRRFRLVTHYWVDDAGV
                     EKTLQALGAVLK"
     gene            complement(21653..23209)
                     /locus_tag="PL000_02850"
     CDS             complement(21653..23209)
                     /locus_tag="PL000_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC87863.1"
                     /translation="MKTKFSSFTPQENLISILPSIVAGILVLVTTVSVSLVYFPPQQA
                     FTRNVILVFGIVGSIYLAFYYILFSASVNKSRFTWSNVIISSATLSTLAFVIPDELDH
                     MLYALVFIASLSTSLISTRAPAYSLVISFTSIYIISRFNTKLETYDWVIHGSQVIAAF
                     MVIETIQQLKKMAREQINKLEIINELSKQIVSTLDTKQVFTLLNAAFQNALEADSYFI
                     GVVEGEEIRLELLYDGGEYFHNVKLPRRGTFSSWVIDNQRELFLPDLRQKIVLEGVDV
                     VLVGQNQTSLSWVGVPMRGTHVDGIMAIASYQPNAFDRGDVELLFTIAQRAALALDNA
                     HHHAIAEEQARLDSLTHAYNHGYFLKLLNQQAQECLDNQQPLSLIMLDIDFFKQYNDT
                     YGHIVGDEVLVSLCRTIQMHIKSTDAVGRWGGEEFVIFLPNADGIQATQIAQRIRESL
                     AAHKIQNNEHANLPIPTISMGIAVFPTETDQTTKLIDLADNRLYKAKERGRDQIEPTA
                     AFWEELNKSS"
     gene            complement(23213..>24841)
                     /locus_tag="PL000_02855"
     CDS             complement(23213..>24841)
                     /locus_tag="PL000_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC87864.1"
                     /translation="LAEAVAAELSRAWERSSYHQRLMNLVQAGSQLSAMVEPAATTRE
                     IATIAREILQARFTFVQIQQGQERNFTESAYSGEAPRLLGSLQESLDSEALIKMAFNA
                     VQPFRVRDVRKYSSTSHLMIDNPVFRSMLAIPIRWHRLSIGVILAFGKQNEVFFNEND
                     ESLAELLSIQAAGAFESAWLQQELRTSLRTTSLLYHLSNKIIQVDNLKDAARDIAQTA
                     HKLAKSITTGVILFNAEGDMVAEVEISSEGIHGGTDHPMEIIRDVMVSGQLIYLSRGQ
                     SIIRACLPIQTPIRTYGAVWMDMPENQGNKHAANPNDLQALVSQTAIALERSLLLVES
                     QRQAVEIKAAYDMLESTYDQTLAALMSALDARDRETEGHSLRVSHLAAKLGKALNFSH
                     DQLKVLERGSLLHDIGKIGISDTILHKPGPLNEEEWKIMKLHPDIGAKIVAGIPFLQD
                     TIPLIRHHQERWDGTGYPDALYGEEIPILARMFAIVDAFDALTSKRPYRERTMSTEEA
                     LDYLRSQSGILFDPNIIEIFETLIKENQPNLLID"
     assembly_gap    24842..24941
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24942..26131)
                     /locus_tag="PL000_02860"
     CDS             complement(<24942..26131)
                     /locus_tag="PL000_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87865.1"
                     /translation="MLFTQTTEQSTEHELDLLTSGIIGIFGVGLLLIPSHLNRFVAGM
                     STWVSVIMGIMAFALALSLQITSTFRSSHKPAIMIRWLNILFLFLFAFLAIRADYFID
                     AIILILISTTQAILATPLRDRFIKFNLLQTCVALIGFFGGLYLILNGDQYAGINLTPY
                     RFPLAAMLMLFPVTWMFFTLSRNHKFNPLVSRLLVIPWLIWCAIFTPPKLVANLIAPA
                     MLCMVVLFEGFIPWNKLAVARNNVLGRKVVMIISTLEMAMLIFLGALFIVIDNFAGEN
                     YSIFAATHEGAFLFFILISIAVYFALFLILVTVNGIMSKLMELDKDDDEDVQSETKAS
                     LWNTRLAHYIEPFINIQEGVRIRLLAQTDQIGVLTQQVKSEKKRNAQLTLLLELSQQL
                     ENQL"
     gene            26305..27141
                     /locus_tag="PL000_02865"
     CDS             26305..27141
                     /locus_tag="PL000_02865"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNC87866.1"
                     /translation="MQNFITLEQIDSVAQAIKKQISIQPIVGIILGSGLNGLADSVRD
                     AVYIPYKDLPNFPVSTVHGHAGRFVIGELEGKPVFVMQGRIHYYEGYTMGQATLPVRV
                     MQRLGIPSMIVTNAAGGVHPDFEPGDVMLITDQLNLMGMSGLNPLMGPNLDEIGPRFP
                     DMSQPYDRAYCDLARAVAKENGLTLREGVYAGLSGPSFESPADLRFLRLAGADAVGMS
                     TVPEVIIARHGNMRVLGLSGISNKANLDGSTITTHEEVIEAGQVITPKVEKIIRGVLR
                     SL"
     gene            27156..27830
                     /locus_tag="PL000_02870"
     CDS             27156..27830
                     /locus_tag="PL000_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87867.1"
                     /translation="MAEIYNFLASYEALIYIVLAIGGLFSFRWLWRSWRESQTAVFTM
                     EREFSSRRLGQAAAISTIIAVLFCAEFFVATFIIPGLPSTALIPTSTLDLISTPTGTL
                     SAEAMTQFANLPPQPASVSLTGCTPNQIMLTSPTPGQEVQGTITLVGTVNIPNFGFYK
                     YEVAPQGGSTWATISAGRETVVNGKLGEWITNALTPGDYQLRLVVTDNQGVSLPACVV
                     PVRVMP"
     gene            27858..28397
                     /locus_tag="PL000_02875"
     CDS             27858..28397
                     /locus_tag="PL000_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87868.1"
                     /translation="MSEIQIRPTIANDLSRLMGFDHTATSDSVWQLELRRDTGQVLAS
                     FREVRLPRSISVAYPRNSFGLADDWAKRSMMYTACIGADPVGYISLLERGMDSMVWVT
                     DLVVDVAHRRQRVGSTLLAAAQDWASGRSYRRLGLEMQSKNLPAIRLAQKFGYEFCGY
                     NDHYYLTQDVALFFGKTLK"
     gene            28455..30365
                     /locus_tag="PL000_02880"
     CDS             28455..30365
                     /locus_tag="PL000_02880"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87869.1"
                     /translation="MINGWMEPLLAGIQALNQILTAGIAITAFSLFLYSLSFNLRDRV
                     ARSFAIILLCVVIVFTAEALQDEAMSSETVDLLLRLQWFGIVFLPAAYLHLSDALLVT
                     AGRPSRGRRRLAVRGMYVVSAFFLALLAFGYLLGPLVPDGKPAPHLQRTMWTEVFTLF
                     YVTAMVWAGVNFARAYSLMLTRSGRRRMLYLMAGATAPALGSYPYLLFGYGLAAEHPY
                     LFWSVATVINVLVGALVIIMAYAVAFFGVSWPDRVIKSRLFKWIMRGPVTASAALALM
                     TVIRRSGELLGSPYNPFVPFIVVVTILSLEHAITFAAPLWERWLFFGRDRGELQLLQN
                     FEERLLTQSDLQQFLESVLAAVRDHMQSPSAFVAALDDETLSPIVVAGNRAMLDQEGL
                     TEALEQIGEDERHEFQWGSFWILPLRHRRRPEMDQDEMPPLLGLLGVAKRDKQLIERD
                     QRDALWLLADRAALALQDRQLQQRAFQSLVDLQPQVEMIQRMRAAVRYDTRANLLTEP
                     LPEEADLANWVKDALTHYWGGPKLTNNPLIKLQVVRELAEKDGGNSANAVRTLLRRAV
                     DQVKPKGDRKFTTEWILFNILEMKFIEGRKVRDVASRLAMSEADLYRKQRIAVEAVAK
                     AILEMEMSRKEA"
     gene            30435..31433
                     /locus_tag="PL000_02885"
     CDS             30435..31433
                     /locus_tag="PL000_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNC87870.1"
                     /translation="MSVQKNKHENPMPVIDEGWWSSVLMEESRYSSAHTPVRSAGNRS
                     EVRSDSKSVASQNDAAMDDPTEKKPATNWRQVKELYMSDQIVDLMVTGHNRGGLLVEG
                     GGLYGFVPFSHLIELAGKVENIDRDHDLEAYVGRSLHLKVIECVPEDDRVVFSERAAQ
                     SEPGKRAELFSALQAGQKVKGTVTNVTDFGVFVDLGGVEGLIHISELSWGRVSHPGMI
                     TKIGNSIDVQVLDLAPERCRVALSLKRLQNNPWESANAEFPEGSVQNATITNVLSFGA
                     FARLDAGVEGLIHVSEIPQANGRPLKEILSEGQPVQVRILHLDSAHQRMGLSMRLE"
     gene            31440..33710
                     /locus_tag="PL000_02890"
     CDS             31440..33710
                     /locus_tag="PL000_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC87871.1"
                     /translation="MTDSSQPRNRQYRQAEPPPIAQNDWLDRFARFYLQFGRFARDIL
                     GVVLFAFALMSLLAVFESIFNQIRSGSETGYNTVMGGVILSFVASRLRLWLGVGSIFV
                     ILGFGYMGYWLIQRDRNEIAWGRLISLELASLLTLGLFAVLGNNDIIAAEAGWDGGRL
                     GWGLVTLAWRLMGKIGGSLVLFLLWIFTIITGFGLWTKIEAWLLKLAGEAPPVVVQTP
                     IPSEPVEQPVEEPEKPAAPKKKAPVIPAEFRTSLKAVDKKDEKPAKPRPRNEDLPPLH
                     ILLAESALRADERTINQTAGLIMKTLADFGIPATVIGYRVGPSVTQFAVQPGFIKKPG
                     TDEEDVQQMKVRVAQIASLEKDIALALSAQRLRIEAPVPGKPYVGIEVPNTHSSVVRL
                     KTLLESEAFNKVGAPLTVAVGRDVSGNPLIADLSRMPHLLIAGATGSGKSVCITSIAA
                     CLAMNNTPEDLRMVMIDSKMVELIRFNGLPHLYGKVETNLERILGVLRWVVVEMEHRY
                     RLLESAHARDLDTYNRKLTRKADGKPLPRIVVMIDELADLMMSAPDVTEHNLVRLAQM
                     ARATGIHLVVATQRPSTDVVTGLIKANFPARLAFAVASGTDSRVILDYTGAESLLGRG
                     DMLFLNPEVGNPVRAQGVMITDMEIERIISHWQKNGDGSEETPPWEKLLQEPGEDEDD
                     GLVEQAISIVRQSQRASASLLQRRLRIGYPRAARLLDQLEEMGVVGPSQGGGKERDVL
                     VSEEDLEEGRQEDLGK"
     gene            33707..34333
                     /locus_tag="PL000_02895"
     CDS             33707..34333
                     /locus_tag="PL000_02895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC87872.1"
                     /translation="MKPTFTDQLRRIFKGILDPTGAFLNRSGLTPNAITLLGLAGTTV
                     GAYILSQGYMTIGGIVLFVSVLVDALDGTMARLRGEPSDFGGFVDSVSDRYAEFITFG
                     GLLYFFLTQADYPGVVATFLATAGSVLVSYVKARAEGLGFTAKVGLLTRVERYIVLIP
                     LLIFNQPFLAVVFIAVLGNITALQRILHVRAQGRERMKKAKENKEYLH"
     gene            34335..35135
                     /gene="lgt"
                     /locus_tag="PL000_02900"
     CDS             34335..35135
                     /gene="lgt"
                     /locus_tag="PL000_02900"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC87873.1"
                     /translation="MTEGLSLGPILLRWNGLLIACGMALGALLSAREAKRHGYDADLI
                     YYLFLPLAVWGTLGARFWHILTPPLSSVQLGLTTQHYLTHALDFIAFWLGGFGFPGAL
                     IGGGLALLYFAKKNELSFWALTDVLAPGLALGQAIGRIGNYFNQELYGLPTDLPWKIF
                     IDPAYRLSGFEAVNFYHPLFAYESLLSLGNLFLLLWLSRRFAETLAVGDLFFAYLGFH
                     SLARFLLEFLRLDVALVNGLNINQVFFAVTFLFSSVSLFWHHRRVREL"
     gene            35204..36139
                     /locus_tag="PL000_02905"
     CDS             35204..36139
                     /locus_tag="PL000_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC87874.1"
                     /translation="MIETHDLSKQFHDFMAVDGVNLSVDSGQILALLGQNGAGKTTTV
                     RMLTALLNPTRGWARVAGYDVVKNGHAVRSSVGVLTEQHGLYMRMNAIEYLDYFGRIY
                     NLEPAIRKTRSDNLLEYYGLTEAAKRRIGEYSKGMRQKLALARAMMHDPGVLLLDEPT
                     SAMDPESARLVRDEIARLKSSQRTIVLCSHNLTEVESLADKIAIIYRGKILLQGSLEE
                     MKRDVLGAPEYEIRFSEVWDAGGLVLPEGVTLLSRTATSLKVRVERPQVSNPIMLQAL
                     SALGAPLLAFQEQARTLEQVYLKVMADAKGSDHVQ"
     gene            36129..37562
                     /locus_tag="PL000_02910"
     CDS             36129..37562
                     /locus_tag="PL000_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNC87875.1"
                     /translation="MFNKLRPVWLVAGRELKDQFRDWRVLTPMIILVIAFPFLMNEFA
                     KQTVDFLNQYEANLILDRLVPFSIMIIGFFPITISLVVALEAFVGEKERGTIEPVLSA
                     PLDDWQLYFGKLLVGVATPLTASYLSIALYLVMVVRQGLGIPSLSVMVQLLFLTTAHA
                     TLMVSAAIVISVQSTSVKAANLLASFIVIPVAILMQGEAVMLFWGNEDVLWLAVAGVM
                     IISLLLIRVGIAHFQREYLLGREIDTINLRWIWGTFWKNFFGGAHSLREWYGMSVGGS
                     LRRVSSAVLVVTLIAIVSIWLGYDWMMVNFAPVIEHATPERLQKITNSAQDFSGLTDL
                     HRTITAPFLFANNTRAVAVIFLAGLVSFSVLGVLLYMVNIGLIGGLYALLKLLGISPL
                     PVFAAGILPHGIFELPAVMIGSAAMLYMGVALVTPQTGKSMGEVIIELFADWLKIFLG
                     LVVPLLAIAAVIEAYVTPVLLVNVMSK"
     gene            37797..38648
                     /locus_tag="PL000_02915"
     CDS             37797..38648
                     /locus_tag="PL000_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC87876.1"
                     /translation="MPKVIILGSSNAIPTKDSENTHMVLVGRERMVLIDSGSNPILRL
                     EQAGLDFNDLTDLILTHFHPDHASGIPLLLMDMWLLGREAPLNIYGLHYTLDRLESLM
                     GFYNWSDWPNFFPVIFHRLPLKEMTPVLNCSDFTIHASPVRHFIPNIGLRVEFAQSQK
                     VMAYSCDTEPCDEVVRLSENADVLIHEATGETPGHSSARQAGEVAKKAGAGRLYLIHY
                     PSGRYASGDPVAEAGENFRGTVALMKDFMVLKFGKRLPLSHFGRAAENSKNSSGKKAG
                     KIYQPEI"
     gene            complement(38630..39133)
                     /locus_tag="PL000_02920"
     CDS             complement(38630..39133)
                     /locus_tag="PL000_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNC87877.1"
                     /translation="MYEPVLVLNANFEPIHICSTRRAVGLILAGKAGLIVNGRGYIRT
                     ISQMLPRPSVIRLESQVHRPRPHVKLTRREIFRRDNYTCQYCGKRDGPLTVDHVIPRH
                     MGGQHIWTNLVAACSACNHRKGGRKVEEAHMHLLHIPKEPPTNAAYIFGRHLTENGEW
                     APYISGW"
     gene            39210..40103
                     /locus_tag="PL000_02925"
     CDS             39210..40103
                     /locus_tag="PL000_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNC87878.1"
                     /translation="MNIMIAGGSGFLGNALAKSFLMDGHKVFILTRGSRTMNGTQAIH
                     WDAKTTDGWGHWVNEMDVVIHLAGKTLASWPWTNAMKKAFHDSRIIPGLALVEAIQKA
                     TQRPGIFIQQSGINYYGLSGDLADESTPPGDDFLSQLAVQWENATKPLEELGLRRVIL
                     RTAPVLDKKEGLMPLMALPVKLFVGGPMGGGKFSAPWIHVQDWVGAVRHLIANEDARG
                     AYNLIAPEPTSNAEFNKTLADVLHRPYWFPVPAFLLRTFLGEMSVLVVEGRFSQPKRL
                     IESGYRYQFPGPREAMTDLFS"
     gene            40123..40800
                     /locus_tag="PL000_02930"
     CDS             40123..40800
                     /locus_tag="PL000_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87879.1"
                     /translation="MKKMILVLAVLLTACGTSSQQHPVVVMPALMATAEPYIDPSYPT
                     AQVEVAAPNQTASGIEVRMDRVSMEGKNINADVCFTLPDTADWGIQSASLTYGGVVVQ
                     EFGTTLLSFQEPVNGAVGLRCDTLTFVVPPDADLTNAAIIIDAIGTTVRADDYCSIYM
                     PKIQQAMLDRGTGISVDCMDVNGMLTMQILSMPPGMTQEQAESIVYSDEFYTVKGPWT
                     FPFGLAQ"
     gene            40974..42185
                     /gene="tyrS"
                     /locus_tag="PL000_02935"
     CDS             40974..42185
                     /gene="tyrS"
                     /locus_tag="PL000_02935"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC87880.1"
                     /translation="MNIEEQVELLMLGTEYGDQDLKKAMTAELRERLTLAQKENRPLR
                     VYCGYDPTSTDLHLGHTITMRKLRQFQDLGHEVTFLIGSYTALVGDPSDKNKARPILT
                     EEQVAENARTYTEQAFRVLDREKTKIRYNGEWLSKLSLVDLIRLGQNFTVQQFLARDN
                     FANRLEKGEPIYIHETFYALMQGYDAVAMETDVQIGGSDQLFNIIVAGRKLQEALGQK
                     PLVGIITAILPGTDGVQRMSKSTGNIVPINTGANDMFGKLMSVPDSAMGLYMRLVTRW
                     SPPEIEKIEQDVASGALHPRDAKMNMAGEITSIFYGEEDAKSARENFVKTFQQKEIPD
                     EMPEFSLQAGQTVVDVILAAGLAESKSKARSLIDQKGVKLDGEVLERGDAVFPHPGVL
                     QVGKRRFVRVK"
     gene            complement(42214..48228)
                     /locus_tag="PL000_02940"
     CDS             complement(42214..48228)
                     /locus_tag="PL000_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC87881.1"
                     /translation="MKNKQPFSALGISLIYAFIGGTWILLSDRLVTLIFTNPDQLSVI
                     QSYKGWSFVIVTAILLFILLKNRRNTLQAAEQNFSDLFESATEGIFRSSPDGRFLRVN
                     SAMAALYGYESPEDMLNLVHDISSQIHASSDGRRKFTEELTKNGYVKNYNAKNLRKDG
                     SIFWTSTNARVVKDANNQVLYYEGFVTDITQQKKGEQSLKEAEERYRTLVERLPSVIF
                     MDNFNDPQSTQYMSPRLKEILGYTPGEWEAGVNMWEKSLHPEDRERVLAEDARTDKTG
                     EPFRIEYRLRHRDGHYIWIKEDASIIKAEDGTPLFWQGTLLDITEQKNSEKVMKENEI
                     SYRELFNSVTQAIYIQDKEGVFLDVNDGAVKMYGYPREYFIGKTPEILSAPGKNDLES
                     LAQKIADTFNGTPQEFEFWGIRSNGQVFPKTVTLNKGTYFGKDVIIAIAQDITTRKQA
                     ESLLERQLKELTVLRATTMAGTQSNSEDDMIAQVTQITAEIYTDVCGILLLNEKGDTL
                     TPHPSYIGANVDKWKNGFPVTQGVSGRAVRSGRAIRIVDVTQDVDYIEIDSNTKSEIC
                     IPFRVQERIIGVFNVESRHKNAFDEKDERLLNTIAGGLGTAIEKLRLSKAEQVQHQRE
                     TALWELIRLANSSLDLNQVLQSILDQLQKVISSDAGSIQLLDKDRLVISAGIGSEILN
                     FSKKGPLLLGKYPLNNYVITEKKPVRLNNVYLDDRFKFFSENSQVLSFMGIPLIVKEK
                     VIGMITLESYQLNHFTKQDEELGLAVANHTSIAIENARLFNEEKRRTQIIETLANIAN
                     EIATTREVIPALDKIAERALSLLKASTLAIYLLQDDEKTIKIVTAKGAYQDQLLSHTL
                     EIGKGITGNIIATGRPEIVNDFSQDTRTVTVPGTPKEDARLDTMMSAPLTLHGKSFGA
                     INAWRPKANGLFDNSELNFLVSIAHQASISIESVRLFEEITRHAQEAAAIAEVGRSIS
                     STLQLNKVLDSIAYYAKELLQAETSAVYLTDPNTPSLRAISALGRESDEIKNDPLKIG
                     NGILGNIALNKQGEIVNNTITDPRSIPVVGTEIERDEHIMGMPFLSKGQLTGLLVVWR
                     VGEDKAFKSTDLDFLNNLAQQATVAIENARLFEAEHKHLQNAEVLNQATSALANTLDV
                     YNLYENILDWLKQLAPYDSASIMMVNSNDTLKMTAMRNIPRHFSIGQIFPLTQKWKAI
                     IESRKPNILDDAQQNFDFQKWTGTEYIRSWMGVAMFIQDKLIGFINLDSKIVGTFTEE
                     HAVLVQTFANQAATALENARLFELEQKRRKESEIMRQATTVLSSLLDLPSLHEAILEW
                     LRKIVPYDSASIMEIEGAHIRITAAKGFQFPEKAIDHTFPIDHVLLKIMNETGEPLII
                     DDCNKDPRFERWGDAEKVRGWMGVPMITRGQVTGYITIDRVIPNTFTKNDALIAQTFA
                     HQAANSLENTRLYTETLQRLDELEMVSRVSFALRTAHDTREMIPILLNEIKANMGTDS
                     AAIWLYDHGKNELVPIATSGRLKDLPKSTFRPNEGIVGAVYSKGATHVSPELSRDILL
                     APENASFFGKGWSSVTLPIRTVSETIGVLVVAMSVPHKIGSNHIRLINTLTEIAGNAI
                     YRSNLFERSEEQIRRLTTLREMDTAITSSLDLHITLGILTEHLTTKMGVSAATVLVFN
                     PNSQTLNYHAPIGFKNREAVRGSQNIGDGLAGQILLNRRAIYIKDLNNEPDLPQMKIL
                     RTEQFVSYYAMPLFSKGATKGILETYFRHSFTPSGDWLDFIHTLAEQAIIAIDNAQLF
                     ENLQRSNQELSLAYDTTLEGWGKALELRDKETKGHTNRVTNLTLELARQMNIPETELL
                     HIRRGALLHDIGKMGVSDAILHKDGPLTQSELFEMRKHPKYAYDLLYPIAYLRPAVDI
                     AYCHHEWWDGSGYPRGLKGEEIPLAARIFAIIDVWDALLSDRPYRKAWSPKKTMKYIK
                     DLSNKQFDPTVVEAFSRMIENDTASKAANEPKKVLKKNPTSRTKKR"
     gene            complement(48337..48407)
                     /locus_tag="PL000_02945"
     tRNA            complement(48337..48407)
                     /locus_tag="PL000_02945"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48373..48375),aa:Cys,seq:gca)
     gene            complement(48434..48505)
                     /locus_tag="PL000_02950"
     tRNA            complement(48434..48505)
                     /locus_tag="PL000_02950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48471..48473),aa:Gly,seq:gcc)
     gene            complement(48533..48605)
                     /locus_tag="PL000_02955"
     tRNA            complement(48533..48605)
                     /locus_tag="PL000_02955"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48570..48572),aa:Phe,seq:gaa)
     gene            48808..52707
                     /locus_tag="PL000_02960"
     CDS             48808..52707
                     /locus_tag="PL000_02960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC87882.1"
                     /translation="MASSLPQKTYARIPVKLDLPNLIEVQLDSFERLKKEGLNDLFHE
                     ISPIESYNKGMKLFFPSRSPESQQWGLKYWFGDPKHGIEECVERDLTYASPLYVSVLL
                     AGPDVPEPIKQDIFLGDFPEMTDKGTFIVNGTERVVVSQLIRSPGVYFEAPADRATGR
                     PLAMAKLIPDRGAWMEFETRKSDYIILKFNRKRTVPITVFLRALAAVDDGLKDSPIKF
                     GTDEEILSIFKDVDNNPERMFIASSLKQEPDWSQYNQTIPNAALIEFFKKMRPGDPAT
                     LDNARQFLEEQLFDQRHYDLERVGRYKLNQKLDLNIPIPHRTVTKSDVIRLIRRMIQI
                     NNGAERPDDIDHLGNRRVKTVGELIQNKLRIGLRRMERVIKERMSIRDQEQLSPVTLV
                     NIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDVH
                     HSHYGRICPIETPEGPNIGLIGRLASFARVNEYGFIETPYRRVYKTFKADDERLEGRT
                     LREDVYDPKSEELLFKSGTSIDAKALKKLAKVGGEISVVPFVSDEISYLSADAEDKYT
                     IAQANAQLTENKEFSRERISCRYHSGFLFAGSEAIDYMDVAPHQVVGISAALIPFLEH
                     DDANRALMGSNMMAQAVPLVRPEIPLVSTGMEGHAALDSGQVVVADADGEVVSVTGST
                     ITVKEKRSGNRVYQLRKYQRSNQSTCIDQRPSVVKGQLIKAGDIIADSSSTDSGQLAL
                     GQNVVVAFLSWEGGNFEDAILLSERLVQDDRFTSVHIEKHEVEARDTKLGPEEITRDI
                     PNVGDDAIKDLDENGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKSRD
                     VKDTSLRMPHGERGKVVDVKVFTREDNSDLSAGVDMMVRVSVAQRRKITAGDKMAGRH
                     GNKGVVSKVVPVEDMPFLEDGTPVDLILNPLGVPGRMNIGQVLEVHLGWAAKRMGFRA
                     ITPVFDGASEEQIEAELARAWIMDQSWKEAAEKAWEWLKDQEYDPNSIQDDDEVRRLY
                     LEHWLGKRKYDVYSLNDLDYARHATAREWLREKGYTNPEEILLEDRDVANPDPEIDDM
                     TVKACLRLWMESLGLTRRVAEDKVYDTAQEVSKEKGVPLPILGKQVLRDGKTGLPYDQ
                     PVTVGVMTILKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAY
                     GAAHTLQEMLTVKSDDVQGRVNTYEAIVKGEPIEEPSIPASFRVLVKELQSLGLAVEA
                     INEGGDVVKFGKDDEKAHPPKHDTGLLSLGENRTGKK"
     gene            52900..53316
                     /gene="rpsL"
                     /locus_tag="PL000_02965"
     CDS             52900..53316
                     /gene="rpsL"
                     /locus_tag="PL000_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNC87883.1"
                     /translation="MPTVNQMVRKGRKNNKTKSKAPALQFTLNSFKQRRVRQDKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGSLDTTGVANRKQGRSKYGAKRPK"
     gene            53341..53808
                     /gene="rpsG"
                     /locus_tag="PL000_02970"
     CDS             53341..53808
                     /gene="rpsG"
                     /locus_tag="PL000_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC87884.1"
                     /translation="MRRAKPEKREILPDSRFNSVNLQTLVQHVLKRGKKSTAVRLIYD
                     AMDLIKERTEKNPMDVFDGALKNVGPAMEVRPRRVGGATYQVPMEVSADRRTTLAIRW
                     LLAAARDRSGKSFSDKLASELIDAFNETGSAIRKRDETHKMAEANRAFSHYRV"
     gene            53859..55934
                     /gene="fusA"
                     /locus_tag="PL000_02975"
     CDS             53859..55934
                     /gene="fusA"
                     /locus_tag="PL000_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC87885.1"
                     /translation="MARVHPLEKYRNIGIIAHIDAGKTTTTERIMFYTGMTHRIGSVD
                     DGTTVTDWMVQERERGITIVSAAVSAEWKGYQVNIIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYGVPRICFVNKMDRVGASYERTIETIIDRLGANPI
                     PMQIPIGFESTFKGVVDLLSMKAIVWEDDLGKEPHVVEIPADLKDQAVEARAKMVEKI
                     AELDDDLTMKFLDAQEISIEELKVALRKGVLSTKAAPVFCGSSLKNKGVQVLLDAVVD
                     YLPSPADRPVITVSEPGNPDNTFDIPAQDDAPLGALVFKIVTDPYVGRLAYVRVYSGV
                     LNQGQTVQNSTKKGRKERIGRLIRMHADHREDVTEIRAGDIGAVLGFKESFTGDTLCD
                     TKALVLETISFPEPVISIAVEPKSSADQEKMGEALRKLAEEDPTLHVNSDEDSGQTIL
                     RGMGELHLDIVVDRLQREFRVPANVGAPRVAFRESITKPIKEVNYKYAKQSGGRGQYG
                     HVVFSMEPGERGSGIVFENKIVGGAVPKEYIGPIEKGVKEAADGGVLAGYPVVDLKVT
                     LIDGSYHEVDSNEMAFKMAAIMGFKEGVQKGNPILLEPMMKVEVVVPEEYLGDVMGQI
                     NSRRGLIQGMEVRPGNAQAVKAMVPLAEMFGYATQLRSATQGRGVFNMEFDHYAPVSQ
                     SVAEEILKA"
BASE COUNT        12842 a        13102 c        15159 g        14615 t
ORIGIN      
        1 tgtcttcgag gctttgtgac tttgtggcaa tcaaaaccca cccttttagc cactctcttt
       61 attcatgctc acttgtccac ttcactgcct gcctttatac ttgccctcat gaacaacatc
      121 gccacccaaa ccattgacct tgccatccag attcaacaga tccccgcgcc gacgttttcc
      181 gaaggacagc gcgccgaatt cacccgtgac ctttttctaa aagagaatct caaagatgtt
      241 tccatggatt cacttggaaa tgtgtatgcg cgtttgcccg ggaagcagaa gaaggccaag
      301 ccgttgatcg tctctgccca tttggatact gtttttccga ccagtatcag cttgcaggtt
      361 aaaaaagaag cgggccgcat cgtcgcaccg ggcatcgggg ataattctct gggtgtggcg
      421 gccttgtttg gaatcctctg gtcgttgcgc gagcggaata tcagcctgca gcacgatctc
      481 tggtttgtgg caaatgtagg tgaagaaggg cttggcgatc tgcgcggaat gcgcgccgtg
      541 gtggagcgtt ttggcgccaa tgtgatcggg tatctcgtgt tggaagggct ggccctgggg
      601 catgtctatc atcgcgccat cggtgtgcgg agatatcgca tctcggtcaa aaccgcaggc
      661 ggacattcgt ggtcagatta tggcaagcct tcggcggtgc atgaactcgc caacctggtg
      721 acgcagtttg cgggaatccg cttgccgcgg gagccgcgca ccacgctcaa tgtcggcacc
      781 attcacggtg ggacggggat caatgtgttg gcctctgaat ccaaatgtga gttggacctg
      841 cgctccgaag acccgaagac acttttgaaa ttgatccatc aagtggaaga cctgctcaaa
      901 gattcagacc gccccggcgt gcgtgtgctg gctgagatca tcggcgaacg ccccgcgggt
      961 gagatccctg ctgatcaccc gctggtgaaa ctggccgtgg attgcacccg cgaacaggga
     1021 ctcgaatccg cgctcacctc aggctccacc gatgcaaaca tcccgctcag cagcggcatc
     1081 ccctcagtag tgatgggaat caccacaggc ggcagcgccc acaccccgca agagttcatg
     1141 gaagtggacc ccgttgaaaa aggcatggaa gctttggtgc ggttnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnaagagt tcatggaagt ggaccccgtt gaaaaaggca
     1321 tggaagcttt ggtgcggttc gtagagttgg tgggggagag aagtgaatag tgattggtga
     1381 atagtgaata gtagatggta aatggtaaat caagtggtat ggtcccttcc cccgttttcg
     1441 atttttgaaa atggggattt ttttacttga agcgtgtctg aaaacttaaa cacaaagtct
     1501 caacacctgc cctggcggtc ggtgccaggg agacacgaaa gcacgaagtt tttttctttg
     1561 cgtctttgtg cctttgtggc ttcgtggtga aaaaggggct tttcctggtt cctggcaata
     1621 aaaacttccg aagcaaatgc acctcggaag cctcttcact attccctact cactattcac
     1681 tattcactac ttcctattcc cctacttcaa ccccagcacc tcaaacactc ccagcggctg
     1741 ggttttgcct ttcacactca tatcggcatg gggtctggcg ttgatttcct tgctgacccg
     1801 ttcgtatgcc gaggcgctga tgaggatctg attccgtgcg gagttttcct gaatgcgttt
     1861 ggcggtgttg acggcgtcgc tgatggcggt gtattcgagt ctcttttcgg tgccgatcaa
     1921 accgagaacg gcgtcgccgc aatgaatgcc tgcgccaaaa ccgagatgga attcaggcgg
     1981 caattcttta tacaggtttt ccaccgattc gcggatgatg agcgcggtct tcacggcgcg
     2041 cagggtatgg tcaggctgcg gaatgggggc gttgaaccag gccatgatgg catcgcccat
     2101 gaacttgtca atggtgcctg cctgcacgag cacggcttcg gccatggcgg cgaggtagcg
     2161 gttcaagatc tgcaccagtt tttctggttc ctgattttcg ctaaaggagg tgaatccgcg
     2221 gatatctgcg aacagggtgg tgatgttcgt gcgtttaccg cccagctgca aactgttcgg
     2281 gtcgagttgt tcgatcacag caggggagac catacgctcg aagagtcggc gctgggcttc
     2341 gagctttttg cgctcggtca ggtcatcaag cacgatggcg atgccctgtg tcttctggcc
     2401 tgcatctttg agcggagaga gattgaaacg ccagtccacg ttgccgtggt tgggcaggtg
     2461 gtggctgatc tcaaggtcta cgatgggttt gtcggtttgg cggacctctg tcagatgcgg
     2521 ctcaagttcg gaggaaacag aagcaagcgt ctcgttaaga tgatgaccga tgatgtcgct
     2581 ggaggaagat ccgaggatgc gttccgcggc gcggttggtg agcgtgatct gattctgaat
     2641 atcggcagtg atcacaccgc tggcgatgga tgcgaagatg ttatccatca gattcttcaa
     2701 gcctgtgacc tcttcgaggg tgttcttgag cgaggagaac agacgggcgt tctcgatcgc
     2761 aactgcggcc tgattcgcaa aagcaaccag caggttccgc tctgaatcag cgaagatgcc
     2821 cgcgcggatg cggttatcgg cataaatgac gccgatgagg tcgtttttta ctttgagggg
     2881 tacgcacaaa atggagcgca ggttgaaggc cacgatgctt tcctgcccta caaagcgctg
     2941 gtcttcctgt gcgttggtgg tgatgatggg ttcgcctgtt tcgatcacac gccctaccac
     3001 tgagcggctc acggtcaact cgctggagtg gatcgattcc atttcccagt tgcgggccat
     3061 gcgggtgacc atttcgcctt tgtcatcgcg cagcatgaga aagccgcgtt cggctttggt
     3121 gaggcgcacg atgttgtcca tcacgatgcg cagcacttcg tcgagttcga gtgatgaatt
     3181 gaccacctga ccgatgttgg caagcgccag catgttgttg tgttcgcgtt ggaaggcatc
     3241 gatcttttcg ctgatgagca tcagcgaggt ttcgatgtga cgcaggtctt cgcccgtgtt
     3301 cttgggcaat ttgagtcggc tttcttccag gtcaatgcga atcttcccca cctgcaggct
     3361 caaggtctgc aactggtctg gaacggttgt aggacgggtc tgttcattca taggcgttta
     3421 ccgtagttga ttataacggt aatcgaagga ttgcaaggct tcttttgcac gaatctgcca
     3481 tgtcttaagt aggataaggg cggcggcttt tcgggcggtt gtaaggcttc ccattttggg
     3541 cgtgtatatg gtggattggt attcctgtaa taaggccagc acttgcggct ctgccgacgg
     3601 catgcgttcg atcaatgctt tggcccgttc ctgcgaagtg atatgacggg gttgcggccg
     3661 cccaagcagg tacaggctga ggttgactgc ctgaaatgtg cgctcaatgg gggagagggt
     3721 ggtccaacgc agccagcgat tgagccagcg cggcggttga tttccgcttt ttgtgtagcg
     3781 gcttgcaagg tacacaggca gtcggtcatc caatgaatag cggcggaagg caaaaacaac
     3841 aaagacaacc gtcattgtca gcagtgaata cagggcgatg gtcttgtaaa gttcaattcg
     3901 gtcggcttgg gtggctccgc tgttttgctg ggtggtcagg ttgggtggtt cttccaacag
     3961 cggaggaacc aacggattgg cggcgggtgt ttcggcggtt acagggtcgg gggtggcatc
     4021 ggggttgatg acatctctgg tcgcggcacg ttcgatgggt aattggttgc ctgtgggttc
     4081 aaattccacc cagccaatgc cagggaaata tacctcaggc catgcatgcg catcctcaaa
     4141 agtgacgaca taacgctcgt caatttcatt aaaggtgcct tcggcaaagc ctactgccat
     4201 gcgggcggga atgccaatgg aacgcagcat caaggcttct gcgctggcat agtacatgca
     4261 gaaacctttt ttgtaatcga aaagcaccca caacacgggg tccctgtttt caggcggctc
     4321 ggcggtgatt tcggtggtgt actcgatctc gttgcgtaaa tacgccgtga tcgcctgtgt
     4381 tttatcaaat ggattgtcgt agggcgcggt gatctcttct gctaatgtgc gcagggctgg
     4441 ggcaatgttt tccgggactt gcagatatcg ctcggtaacc cactctggat agtcagtgcc
     4501 ggcggcgcgc aagtcttcct gagtggggtc tgcgatcaac gcgcggaccg tgtacctgcc
     4561 tccgctcgaa acacccgggg tggcgttcca ggcagtgaca tcctttaatg aagagccgat
     4621 ctcagttgcg gttaccgtcc cttcgcggct gacccagatc gtttcggatg ggccatacag
     4681 caggttctgg cttgtcaaat tgttggtgaa ggtgaattcc atttcaaagc ggttctctgg
     4741 atattcaact tccaattgat cggtatctgg cacgaaaggc gtatttgaat ctgaaagtgt
     4801 cgtccagcgg ccattgatgt acaggtcgta cattctgcct ttccaataat tgcggatcgg
     4861 cacaaattca ctttccttca ctgtgacgaa gaaaacgggt gtatcacctt ctgcagcctg
     4921 ctgacccagc gaaagttcgc tgctgtaaaa ttccccgcct gagttactgc ccgcatacgg
     4981 tgattccagg gcgctgaccg catcggaaag cctttccagg atcggctcgg aaatttcgcg
     5041 ccaggccttc gctgcaggtt taatttgctt gatccaaccc gggatggacc aggctacgaa
     5101 taccgtgatc gccgccacgg tcagcgcgcc gcgctccagg ttgttgatgg tttcagccgt
     5161 gagcaggaag cgggtcttcc tccaataggt ttgattttca aggaaataca agcgcccaag
     5221 caggagcaat gccgcaaata aatagaaggc caatgcccac atgcgccgcg attcggtcgg
     5281 gtcgaagatc tgcaccacca acatcgccag acatgaaggc aggatgacgt ttagaaaatc
     5341 gcgatgacgg gtgagccagt agcccgccga aatgccgatg atccaatagc ctgctgtcac
     5401 gagcgaaata aagaacagct ggtcatatac gggctggcct ttgatgaatt gcgccatcga
     5461 agtgaacaga cggttaaaca ggacgaccag atcaggccaa acccagtcgg ttctttcaac
     5521 ggcatttaat aattgtgcag ggagcagaat gagcgaatat cccaccgcaa tgcgtgtggc
     5581 tgcttttcgt gaaaaaatgc tgtagcccag tgcaaaaccg agaatgacac ccgccaggct
     5641 gatggtttgt gtgaaataga gatagggaac ccattcagtg atgaccagcc gtgagcttga
     5701 aatttcgatc aaggcgatcg tcaacagaat ggcgcgccaa tcccatacac tggttcttgc
     5761 agatgactca gcgttggagt tggtgatgga tgatttttcc atggtttgag tgtacaatac
     5821 aagaagcccg ttcgtcaatt gtacaacccc aaaaaaccct actggaggat ggtatgcaga
     5881 ccttaattga gggaggtatc gcccttatta tcgcgattca aagcctgggg gattggttaa
     5941 ttgctcccat gaaatttttc agctacctcg gcaacgagga attctttttg ctggttctgc
     6001 ctcttttata ttggagcgtt gactctgcgc tgggtctgag ggtggctttc attttggtaa
     6061 caagcaattt atttaatcat gtgggtaaat tattgttcgc cgggccgcgc ccctattggg
     6121 taagttccga tgtacgcgca ttgtggaatg aaatttcatt tggcatcccc tccgggcatg
     6181 cgcagatcgc agcgagtgtg tgggggatgt ttgcggcgta cagtaaaaag aattgggtat
     6241 gggttggttc gatcacggtt attttcctga tcggtttttc acgtttattt ctcggcgcgc
     6301 atttcctgca tgatgtgttg ctgggctggc tgatcggtgc ggcgatcctg tgggcctttg
     6361 tccgcttttg ggattcggca gcggcctggc tggagaaaaa atcaatgggc gggcagatca
     6421 tggtcggttt tatcgtttcg ttgattttca tcgccatcgg atttggcgct tcgctttttc
     6481 gaagcggatt ccaggttcct gaaacctgga tcgcaaacgc ggctctggcc gggaccgagg
     6541 tgcctgctcc gctcgacccg aacagtacct tcacctcagc agggacgctt tttggcgtgg
     6601 tcattggcgc ggcgtggatt cactcctttg gcgggtatca ggcttcaggt ccggtggcga
     6661 agcgcgccat tcgttatgtc atcggcctta tcggaatcct catcctgtgg atgggtttgg
     6721 gggcgatttt cccgcgcggc gagggattca ttttttacac cctgcggttt atccgttaca
     6781 cgctgatcgg ctggtgggtg gcaggcggcg gtccgtggtt atttgtaagg ttaaagctgg
     6841 cctcatggaa tacgggcgag aattccatct gatataatcg cgccattccg tataaatcca
     6901 taagggttcg tagatgacgc tagagaaaaa caccctccta cataatcgtt atcgtatcat
     6961 tgaaattctt ggccagggcg gcatggggtc ggtttaccgc gccattgatg aaaatcttgg
     7021 cgtggcagtt gctttaaaag aaaacctgtt caccactgag gaatataccc gccagttccg
     7081 ccttgaggcg gtgattctgg cgaatttgcg ccatcccaac ctgccgcgtg tttccgatca
     7141 ttttgaattg ggcgagcagg gtcagtattt ggtgatggat tttattgaag gcgaagacct
     7201 gcgtttccgc atggaacgcc ttggtaccat cactgaagaa gatgcggttc agattggcgc
     7261 ggcgatgtgc gatgcgttgg cttatctgca cagccgcaag ccgcccatcc ttcaccgtga
     7321 tatcaagcct ggtaatgtaa agatcacccc ggatgggcat atttttcttg tagatttcgg
     7381 cctcgcgaag gtatatcagg gggccagtca ggctaccact actggcgccc gcgcgatgac
     7441 gcccggatat tctcctcccg aacaatacgg cacggcacgc acagatccac gcaccgatta
     7501 ctattcattg ggcgcaacct tgtatgccgc cttgtgtggt gtcatccctg aagatgggct
     7561 tgcacgtgca atggataacg cgcaattgac tccgctgcgg aaacgcaacc cgaagatttc
     7621 gcgccgcttt gctgctgcca tcgaaaaagc catggccatt gatccaagcg accgttttca
     7681 aaacgcagag gatttcaaga aggccttgtt gggttcgagt tcaaaatcgc agtcgcggcc
     7741 gggtggttac acagttacgc caccgcctgc cgaggcgttt ttgccgaacg atcccagtga
     7801 agttgtagca gccaaagaaa aggaaaagac tccgccgcgc caaatgaccg gccccgtgca
     7861 gcctgcatct gcagaggaag agcagccttt tgtctcaccg cttaagaaac aaaaagaacg
     7921 tgagcgcaaa cgtagaaatg cgttgatgcg ttttatggct gttttgattt tattggctgt
     7981 gatcggtgtt ctcgtatttg ccccgggtgt tatccctgca gatcttcgtg ctcaaatacc
     8041 tttccttgcg ccgacgagta cagccacttc ttcgccaacc tcaactgcgc tgccaacctt
     8101 tacgctgaca tccacatcca ctgcgaccga tgtccctcct accgcaacca gaaccaaggt
     8161 tccgccaact cataccaata ctccaccgcc aaccgggaac attcctgttg aagctacttt
     8221 gacgaatacg ccttcggtta cttcgacccc cactcttgtt cagccttcgc cgacaccttc
     8281 tctgtcggtt acgccgatcg gcggcggttc agggcagatc gcttatgctt caaaccgtac
     8341 cggtttgccg caaatttacc tggcagatat cgccagccag accgcaaccc agatcactga
     8401 gatgcctgat ggtgcctgcc agccttcgtg gtcgcccgat ggacagaggc ttgtcttcat
     8461 ttctccctgt aaaggcatgg atgaaatcta ctatcaagcc gccctgtaca ttatcaatgt
     8521 agatggcact gatttgaccc cgattgccac cgtccccggt ggagattttg acccagcctg
     8581 gtcgccggat ggcaaatcca ttttgttcac gtctctgcgc accggacaga tggagatctt
     8641 catggtcaag ttggatgatc tctcgaatgc cattgaagtg acgaagggcg ccaagagtgt
     8701 tgagtcgcgc cagccttcct ggtcaccgga tggcaagcag atcgtctact cggtcaaacg
     8761 ctttggtgtt taccagatct ggttgatgaa tgcagacggt acggagccaa aacagatcgt
     8821 tcgcagcggt gtggcatttt cagattacct gcctacctgg tcaccggatg gaagtttgat
     8881 cctgttcaac cagcgctgtg ccacttcgtt ctgtctgccg tatttgatga gtatttcctc
     8941 cacagatcgc tccgtggagc aggggacttt gctccaattg ggtgtaactt cagtggaaga
     9001 tgttcattat tccccggatg gttttcactt gctgtttgaa ggtgaagaag ctggtgagaa
     9061 taatgacatc atctatgtga cggttaccgg cgcaaatcgc gtccgcatca ccacagatcc
     9121 aggcttggat tttgatcccg tttggcgacc aactccaaaa taagtacttt cacctagaat
     9181 ccccaagcgc cagagtataa taccgtactc tggcgcttcg attttattga gtcatctcat
     9241 gtgtggcagg tttttaatct ttatttgcta caataccttt caaaaatatt aataagcaat
     9301 tttttgaatt gaaataaaat agtattgaaa tagaacagaa atgaaaactt tccttcttga
     9361 gagtagaata cctttcgaat tatgaaatga aaaaatgcag acaggaattt gatgattctc
     9421 gaaaacgaaa ctcttttaca taaccgctat cggattctag agaatcttgg gcaggggggg
     9481 atggggtcag tgtaccgcgc tgtggatgaa aatctcagcg tggatgttgc cgttaaagag
     9541 aacctgttta caacagagga atatgcacgt caattccgcc ttgaggcggt cattcttgca
     9601 aatattcgtc atccaaatct gccccgcgta tcagatcatt tcgtcattgc cggggaaggt
     9661 caatacctcg tcatggattt cattgagggg gaggatttac gccaacgcat ggaacggttg
     9721 ggggctcttc cagaagatga ggttattctg gtcggtgcgg ctatttgtga tgcgcttaca
     9781 tatctacatg cccgtaaacc ccctattctg catcgtgata taaaacccgg taacgtaaag
     9841 atctcacctg atggacacat tttccttgta gattttggtc tcgccaagat catgcagggg
     9901 aatcaggcca ccaccaccgg cgcgcgcgca atgacgcccg ggtattctcc tcctgaacaa
     9961 tatggcaccg cccgcaccga tgcgcggaca gatatctact ctcttggtgc aacgctatat
    10021 gcggccctga ccggtgtcat cccggaagat ggccttgccc gcgccatgga taacgctcaa
    10081 cttacaccgc tgcgcaagcg caatccagac gttgcccgcc gccttgcggc tgccattgaa
    10141 aaagccatgg aagtggatcc tgctgatcgt ttccaaacag cagaagaatt caaactggcc
    10201 atgctcaatg ccaaatccag gacccaacag ttgcttggaa cttatacgat tgccccccct
    10261 ccttccgaag cggaaagaaa aagcgatccc gttaaggccc ccaatatcat taaacactct
    10321 tccgctgctc tggaagatca ggaatttgtc tccccgcgga aaaaattact cgaacgtaac
    10381 cgtcgcaaca tgcgtgccct cgtgggtggc ttgcttgcgg tgggttttgt tgtattttta
    10441 ggcatcatgc tttttgttcc aaatgccatg ccggaatcgg tgcggagatt gttcccattt
    10501 ttgccgacca ccgaacccac aaccatccca acagaaattg catctatacc tccaacccct
    10561 gtcgcagttg tggtggatga accgactgcc acactcgtag tgtccacccc ggttcaggta
    10621 acacccacct catctgtgaa tcaagccgtt gaacctaccc ccatcggtgc agattacctg
    10681 cagatcgcat acgtttccac ccgtactggg ttaccccaga tctatctcac caatattctt
    10741 accgatcaat ccattgctgt taccaatatg acgggcggtg catgtcagcc agcatgggct
    10801 ccagatggaa gcaaattggt tttcatttcc ccctgcgctg taaaaaagga tacctatcct
    10861 gaggcgagtt tattcatcat caactcggat ggcacaggct tgcagcccct gccgacagcc
    10921 ctcggtggag attttgagcc cgcctggtct cccgatggaa aatccatcgc ttttacttcc
    10981 ctgcgcgatg gatatatgca gatctactcc tatgatattg aaaacgacaa gattcaacgc
    11041 ctcgttgaaa cagatgccac caccgctgcg cgccagcccg cctggtctcc cgatggcaga
    11101 cagcttgttt tctctttgaa gcgtgttgcc acttatgaac tttggctgat gtccagcctc
    11161 ggcgccaatg aaagactact tttcacaagc ggcaacgtat tctcgaatca ataccctgtg
    11221 tggacgcctg acggaaaatt cattctcttc agccagcgcc gccctgaaga agcgaccttc
    11281 cccaatttgt tctcacataa aattggcgat aacaatgatg ccgccctcaa acttgcactg
    11341 agtgtccgct cggtggaaga tgtggattat tctcccggca gtttttggat cgcctacgaa
    11401 agcggtggcg accgcggata tcacgtctat tactccaccc cggcaggcgg caacctcacc
    11461 cgtgtcaccg aagatgtcac ctttgacgat ttcgaccccg cctggagacc catctccaca
    11521 ccctgagttt cgccctgata caacaaaaga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn ntttttctta taaactctta cactcctctt attgacgccc tccatttcca
    11701 catgtaaact tgcattccga tgccagaact taccgaaaga cagcggacca tcttacagct
    11761 cctcattcgc gactatattg agacggccca acccgtcggc tcaaagcgat tggtggagcg
    11821 cttccatctt gacctgagtc ctgccaccgt ccgaaatgaa atgtcagcgc tgacagagat
    11881 gggctaccta agacagccgc acacctcagc gggtcgtgta ccctcggaag atggctatcg
    11941 ggcttttgtc ggtgagatga tccacaaagc ggaattgccc gcttcggtgc aaaacaccat
    12001 ttcgcatcag ttctttcaag cccgccccga tatggaccag tggatgaccc ttgccgcttc
    12061 gatcctggcg aatcaatcgc agggcgtttc ggtggtcacc gcaccccatg cagagaccat
    12121 ccgtttcaag catattgagt tgatctccac ccagggacgt caggtcttga tggtcttggt
    12181 catggatggc ggcgaagtca accagcagat tttgacattg gctgaaatcg tcacacagga
    12241 aagactcagt caggcggcgg ctcatctcaa ttcgctgctg gccggtcttt caatggactc
    12301 catcgctgcg gtttcatccc gcgctgacgc actcgaacag gatatcatca ccatcctgtt
    12361 acaggatatg aaacgcactt ctgaaagcgt ttcaggtgaa atttataccg acggcctcac
    12421 caatgtgttg gatgaacccg aattcgcaga atcggacgag gctcgttggg ctttgcgtct
    12481 ttttgaagaa cgatccacct tgcaggggtt gctggcgcgt gcatcttcca atagcagcgt
    12541 cggtgggttg caggtattga tcggcggctc cggcggctgg gatgaattgc gtcaatgctc
    12601 gatggtggtc gccagatacg gtgtccccgg gttgttgacg ggtacgctcg gtgtgttggg
    12661 ccccatgcgc atgtcctatg cgcggacgat ccccactgtg cgttatgtgg ctggcctgct
    12721 aagtgatctg gtgaacgata cgatttctgg tgagtaaaaa aacgtaattg gtcgtttgtg
    12781 gatgaaattg ccaattacga attaccaatt tgagagtgag aaacatggca gacgagaaag
    12841 ataaacagga agaaattgat gagactcaac cctcagccga agaaatgccc gttgagaatg
    12901 ggcaggcctc agcggagcgc gaagcgttga tcaatcaatt gaaggaagcc gaagccaggg
    12961 tcatcgaata taaagatggg tgggcaaggt ctcaggcgga attccagaat tacaagaagc
    13021 gcattgagcg tgataacgaa ttgacctatg ccagtatgaa aggtgacatc atcaagaagg
    13081 tgttgcccgc gcttgatgat ttggagcgcg ccttgcaaaa ccgccctgcg gatgatgcat
    13141 gggcaagcgg cattgagttg atcgcacgca agatgcagaa tattttggaa ggggaaggcc
    13201 tgaagcgcat tgatgccaag ggaaaaccct tcgacccgaa ttttcatgaa gcgatctcgc
    13261 acgaacccgc cgatggtgtg gagagtgagc atgtgatcga tgtagtgcaa aacggataca
    13321 tgcttggtga tcgggtaatt cgccctgcat tggttagagt ggcacagtaa attcggttaa
    13381 ggttgaaggt tacaggttgc aagtttgtac gaaccttcgg ctttcaacct tcaacttgaa
    13441 acttaggaga aatcatggca aaaatcattg gtattgatct tggtacgacc aactcggtgg
    13501 ccgctgttat gtcgggcggt gagccgattg tgattccatc tgctgaagga gagcggcttg
    13561 tcccttcggt ggtggcgatg aataagaaca gtgagcgttt ggtcgggcgt gtggctcgca
    13621 atcaggcgat caccaatccg cagaatacga ttttttcagt gaagcgtttt atgggccgta
    13681 aatctgacga ccctgaagtg gagcgcaccc gcaagcgtgt gccttacgcg gtgaacgcgg
    13741 ctgataacgg cgatgtgcgt gtgcgcctcg gcgaacgcga ttactctgcg cctgaggttt
    13801 ctgcgatgat cctcgcgaag atcaaggctg atgccgaagc ctatcttggt gagaccatca
    13861 cacaggcggt cattaccgtc cctgcgtact tcaatgacgc ccagcgcaat gccacaaaag
    13921 atgcgggcaa gatcgctggc cttgaggtgt tacgcatcat caacgagccg accgcttctt
    13981 cactggctta tggccttgat aaaaagaaga acgaagtgat cgtcgtgtat gatctcggcg
    14041 gcggtacatt cgatgtttcc attttggatg tgggcgatgg cgttttccag gtccgtgcta
    14101 caagcggcga taccttcctt ggtggcgatg atttcgatct tcgcatcatg gactatttga
    14161 tcgaacagtt caagaaagat aacggaattg atttgaacaa cgatcgtcag gccttgcagc
    14221 gtttgaagga ggcttctgaa aaagccaaga tcgaactctc cactgtgatg cagaccgaaa
    14281 tcaacctgcc gtatctgacc gccgatgcga atggccccaa acatttggtg atgactctct
    14341 cccgcgccaa actggagcag atcaccgcgg atcttgtcga ccgaacgatt gctcctgtca
    14401 aacaggcatt atctgacgct ggattaaacg caggtgacat caatgaagta gtgctggtgg
    14461 gcggcatgac ccgtatgccc gccattcaag accgtgtacg tgcctttttc ggcaaagacc
    14521 cccataaagg cgtgaatccc gatgaagtgg ttgccattgg cgctgcgatt caggctggtg
    14581 tgctcggtgg ggaagtgaag gacattctcc ttcttgacgt aactccgttg actctttcca
    14641 ttgaaacctt gggtggagtt gccactccac agatcgagcg taacaccacc attccaaccc
    14701 gccgcacaca ggtattttcc acggcaggcg ataaccagac ccaggtggag atccatgttt
    14761 tgcagggtga acgccccatg gcgaacgata acaaatccct gggcaaattc atcctggatg
    14821 gaattccgcc tgcgccgcgt ggtgtgccgc agatcgaagt gacctttgat gtggatgcga
    14881 acggcatttt gaaggtcagc gcgaaagata aagccacggg tcgttcccag cacattacca
    14941 ttactgcatc ctcaggcttg agcgatagcg aagtggaaaa gatgcgcaag gatgccgaag
    15001 cccacgccga agaagaccgc aaacgcaagg acttgatcga agcccgcaac aacgccgaca
    15061 acactgctta tgctgccgaa aaagctctgc gtgaattcgg ggataaaatt cccgccgatg
    15121 tgcgtgctga cgtggaagcc aaagctgcag aggtcaaatc tgccgcacag ggtgaagatt
    15181 tggaaaagat caaagccgca actgaggctt tggggctgtc catccaaaag atcggtgcct
    15241 ctgtgtatga gcagggacag gccgcgggtg gcggtgaagc gggtgaaagt tcatccgctg
    15301 gccctgacgt ggtggatggt gaagtcaagg agtaggtcgg tttaaggttc aatgtgacag
    15361 gttgaaggtt tgtaacctgc aagttgtaac ccgcaacctg taacttaaat tatgagcaat
    15421 caggattatt acgaaactct gggtgtggga cggaacgcga gcgatgatga agtcaaagcc
    15481 gcgtttcgta aactcgcgcg ccaatatcac cccgatgtga acaaggaacc cgacgccgag
    15541 gaaaagttca aggaaatcaa cgaagcctac ggtgtgcttt cggattcgga caagcgtgcg
    15601 cgctatgacc ggtttggcaa ggaaggtctt ggcggcatgg gcagcggtgg cttccacgac
    15661 tacagcgccg atttcggcga tatcttcgaa gacctgctga gcggattcgg cttctcctct
    15721 ggccgtcgtt cacgcaacag cacacgcccg cgccgtggac gtgacctgca aatggccgtt
    15781 tcgttgactt tcgaagaagc ggtctttggc gtggacaaag atatcgaatt cgagcgcgaa
    15841 gaaacctgtt cacgttgtaa tggcaatggt gccgagccag gcacatcgcc agtgaaatgc
    15901 tccacctgta acgggcaggg tgaggtccgt caggtgcgcc agacctttct gggtcaaatg
    15961 gtgcagacag cagcctgccc aacctgcaat ggtcgcggtg aaacgatctc ctcgccatgc
    16021 aaaacctgtc ggggcggtgg tctggaacgc aagcacatca aaaagaaagt gcagattccc
    16081 gccggggtgg accatggcac acagattcgc ctgaacggcg aaggcgctcc cggtgtgtat
    16141 ggcgggccca gcggcagcct gttcctggtg ctggatgtaa agcagcatca attcttcaaa
    16201 cgccgtgata atgatgtggt cttgaacctc gatatcaatg tggcacaggc tgtccttggt
    16261 gccgaggtgg atgtgcccac cctcgatggc gaagagaaac tgaaaatacc tgctggtacc
    16321 caacctggca aggtcatcac catgaagggg aagggtgttc cgtatctgcg gcgcaaagat
    16381 cgcggcaacc aactggtcat tgtcaacgtg gcgatcccca ccaaactgac aaaggaacag
    16441 catcaattgt ttgaaaagct ggctgagagt ctgggtactt cggtcaaacc gcaggaaaaa
    16501 ggatttttgg attggcttaa tgaagccttg ggtggatagt ttttacagta gaacagcaac
    16561 tgataaacga acaacgagca ggtgatgaac ctgctcgttt tgcttttttt cgcctgtgca
    16621 tccgtatcga gacgagagca caactgtgaa ttagaacaaa ttttctgtta taatgactaa
    16681 aagattggcg cggctgatca aatttatcaa tggaggctga gatggatttt agttctataa
    16741 actggttggc agtggtggtt tgtgttttgg caagtatgat gatcggtggt ctgtggttcg
    16801 gtcccaagac ctttttcccg atctggtgga aggctgtagg caaagcagat gttcaagcgc
    16861 caggaagtgg cacaagcatg gccattgtct ggggactgat tgtggtctct tcatttgtgc
    16921 aggcgatgtt tatggcgctc atggtcaatg ccatgggaag cctgtcgggc ggtgccacac
    16981 ttggctctgg cgcattgaca ggctttttgc tttggctcgg ttttgtagcc ccctccagcc
    17041 tgacgaataa attatttgca gaccgcataa aagcctggat gatcgaagtg gggaaccatc
    17101 tcgtcacttt tgttgtgatg ggtgccatcc tcggcgcatg gcattgatca gacctgtaca
    17161 accccatacc gactcatccc gaacccagtt tcgtggatga gtttttttat ttccccgcta
    17221 tgatcacatt gcactttgtt cttgggcgcg gatttttttg aggattctgc cgaagacctc
    17281 ccaaaatcag cgttttcagc gttcatctgc gtcccgaatt taatagcgta aggtcatcaa
    17341 ttccttgcca ctgatttcct gacccgttcg gttatactcc acccatgcca aacaccatcc
    17401 gaccctacaa aacagaagat tttgaagccg ttacccgcct atggttcgat gcccaaaccg
    17461 ttgccatgcc cgaaatgatg gcccgcatgg gatatgaatt tagcgaggca caggaatatt
    17521 tccgcaatat cgttgtcaat gaaaaccaga tctgggtata tgaacgcgat ggcgaacccc
    17581 tgggttttct cgccattcaa ggcgaactca tcgaccgcct gtatgttcac cctgcctggc
    17641 accgtcaggg cattgggcag gcattgctca ataaagcccg cgaacttttg cccaaacaca
    17701 tctggttata cacccacacc gccaaccgaa tggcgcgggc attttatgaa aaaaatgact
    17761 tcatcgccga ggggtttggc tccagccctc cacccgaatc cgaacccgat gtggagtatc
    17821 actggcgggc aaagtaaagg tgaaggtgcg ataaaataaa aaaatgcgtc ctacatttct
    17881 tgaagtcaac ctcacccaac taaaacaaaa tcttgaaaac attcgcgcaa aggtagcgcc
    17941 tgccaaagtc cttgtggtgc tcaaggccaa tgcctatggc cacggagttg atggcgtggc
    18001 tcccttcatt gccccctttg ccgattacat tggtgtggcc attgccgaag aaggcatcca
    18061 tcttcgcaaa atgggaattg cgactcccat cctcgtgatg ggtgggactc tcccgcagca
    18121 gctgcctgat ttttttgaat acgacctcac cctcgccgct tcttcattgg acctgttaac
    18181 tgctgccgaa caattggcag cctccacccg taagcgactc aagatccact tgaaagtgga
    18241 tactggcatg gaacgcatcg gtgtccgcga atacgaagcg gactctttaa ttgaactggc
    18301 tttgtcgtgt agccacttgg atttggaagg catctttact cattttgcaa actcagaagt
    18361 gatgtcgttg cagaaaaata tgaccccgct ttccttgcag cttgaacgct tcgaagaaat
    18421 ccttgctttg tttgagaaga ggggggcgcc gcagccacgc attcggcaca ctgcgaattc
    18481 gggtgcgatc ctgttgcacc ctgaaagcta ttacgacatg gtgcggcccg gtgtcttgtt
    18541 ttatggtgta taccctggcc gtgatgtgga aaagaccatt gaggtccgcc ccgcactgac
    18601 ctggcgctcc aaggtcgttt acagcaagat cacccagccc gggcgtggag tcagctatgg
    18661 ttcgttgtgg caggccgaag cggagtcacg agtcgttact gtcccgtgtg ggtatgccga
    18721 tggatacttc cgccgcatga cgaatcaagc gcgggtgaat atcaacggca ggctctatcc
    18781 gcaagtgggg cgcatctgca tggatcagtt tatggtcaat gcaggggagg atgttgtgaa
    18841 ggttggggat gaggtgattt tgctcggcgg cggcatcaca gccgaagacc tggccgattg
    18901 gacgggtacg aatgaatatg aagtaatgac caacatcagt gcgcgtgtgc cgcgtgtatt
    18961 tacccgtgcg gtacaatagg cgcacaaatt tgaaattgag gtgaactttg aatcgtgttg
    19021 tgaaggatta tctggtttta tttggcgtgg ggggcacaag catcgccttg gatcagtgga
    19081 cgaagtggct tgtacgaacg aacattgaat ttggcatgca gtggctgccc gactctttga
    19141 tgtggttgag cccatatgcc cgcattgtgc attggtacaa caagggtgcc gcctttggta
    19201 tgtttcaaaa tgggaacatc gtgttcacca ttctggcttt tattgtgatc ggcgcgatca
    19261 tttattatta tccgcgggtt gaggcaggtg attggacttt gcgtctggcg atggggttgc
    19321 agcttggcgg tgcggcgggg aatttgatcg accgtctgcg cgttgggcag gtgacggatt
    19381 ttatttctgt cggcaccttt cctgttttca atattgcaga tgcatccatt tcggtgggtg
    19441 tggctgtgct tttactgggg gtgtggctga atgagcgcaa agaaaaactc aatgccgcgc
    19501 aacaatccac cccagagggg gagcagcctg cagcagcagt tttgaatggc gttggccccg
    19561 tggatggagt ggctggcggt gaacaaccca aatcgaatgg cgggcaatcg tgagtgaccg
    19621 tgttgaaaaa ttcgtatatg acggagaaaa agcagatcgc cttgataaat ttctggtgac
    19681 ttgcctgcct gagttttcga gggcgcggtt gcagggattg atcgatgaag gttttgtcac
    19741 gatcaacaat gtccgcgcga agaaatcggg gcaatccatt gatccgggcg cagagatcga
    19801 agtgcgtatt ccgccgcctg tgccgagtgg tttggtgggg gaggatattc cacttgatat
    19861 tatttttgaa aacgatgatc tgttgattgt gaacaagcct gcaggcatgg tcgttcatcc
    19921 tgctgccggg catgattctg ggactttggt tcatgcggtg ttgggatatg accccgacct
    19981 cgaaggcatc ggcggcgagg aacgtcccgg tctggtgcat cggttggaca aggaaacatc
    20041 gggtctgatc gtgctggcga agaacgagcg cgcacaccgc tggttacaag accaattccg
    20101 tttgcggacg gtggaaaaga cctatcttgc attggtggat ggtatgccgc ccactcccac
    20161 cgggcgtgtg gaggcttcga ttggccgcga cccgagtcac agaaagaaga tggccattgt
    20221 tactgcgggc aagggacgtg aagcggtcag tgaatacaaa acgcttgaag cgttcaagaa
    20281 tcatacgctt ttggagtttc atccgcacac agggcgtacg catcagatcc gtttgcactg
    20341 tcagtttttg gggtgcccga ttgtgggtga ttccatttat gggcgcaaga atccgactgt
    20401 tgaccttgac aggcattttc tgcatgccta tcgtttgaag atcgttttgc ccggagaaaa
    20461 agaaccaagg gtgtttactg ctgctttgcc cgaagagttg gaaggtgtgt tgaccgattt
    20521 gcggggttga ggaaatcaat tcatggaatg aacgtggact gtttccgttt tttggggaag
    20581 aggcagcaag tctatttaaa ggagtttgtc atggaatttg tggatcttcg ttctgatact
    20641 gtcacaaaac ctacacctga gatgcgtgag gcgatggccg aggcggaagt gggtgatgat
    20701 gtttacatgg atgacccaac cgtaaacgcg ttgcaggaaa aagccgccga aatgctcggc
    20761 aaggaagacg cgttgtttgt cccttcggga acgatgggga acctgctggc tttgctggtg
    20821 cattgtcaac gtggggatga ggtgatcgtt ggtgataaat cgcatatcta tttgaatgag
    20881 gcgggtggaa tgtcggcact gggcgggatt catccacggc ctttgcagaa tcaagccgat
    20941 ggcacgcttc tgctggatga gattcgcgca tccatccaaa ccgaggatgt gcatcacacc
    21001 attacgcggc tgatctgcct tgagaatacg caaaatgtgt gcggcggggt tgtgttatcc
    21061 gcggagtatc tgcaggcggt tggaaagatc gcacaggaaa acgggttggc tttccatatt
    21121 gatggtgcac ggatttttaa cgcggcggcg gcattgaata tctccgtgaa ggatctggtt
    21181 gcgcctgccg attctgtgat gttttgtttg agcaaaggat tggttgcgcc tgtggggtcc
    21241 atgctggtgg gggggaggaa attcatcaac cgcgcacgtc atcttcgcaa gatgctgggt
    21301 ggggggatgc ggcaggcagg tgtgttggcg gcggcgggga tgatctcgtt ggagaaaatg
    21361 tctacgcggc ttgggcagga ccatgcccgg gcgaagaagc tgtttgaggg gttgcagcag
    21421 gttcggggat taaagctgga tgcgttcggc ccttcatcca caaatatggt ttattttgac
    21481 ctggctgatg aggtcaaatt aagtgttaat caggtcgttg aagaaatgaa gaaacacggt
    21541 gtgttggtgg attgggctgg cccccgtcgg tttcggttgg tgacgcatta ttgggtggat
    21601 gatgcgggtg tggagaaaac cttgcaggct ttaggcgcgg ttttgaagta gcctaactgc
    21661 ttttattgag ttcttcccag aacgcggctg tgggttcgat ctgatcccgc ccgcgttctt
    21721 ttgctttgta gaggcggttg tcggcgaggt cgatcagttt tgtggtttgg tcggtttctg
    21781 ttgggaagac ggcaatgccc atgctgatgg ttggaatggg aagattggcg tgttcgttgt
    21841 tttggatttt gtgcgcggca aggctttcac ggatgcgttg tgcgatctgt gtagcttgta
    21901 ttccatcggc atttggaagg aaaatgacga attcctctcc gccccagcgc ccgaccgcgt
    21961 ctgtgctttt gatgtgcatt tggatggttc tgcacaggct gacgaggact tcgtcgccga
    22021 cgatgtggcc gtaggtgtcg ttatattgtt tgaagaaatc aatgtcgagc atgatgaggc
    22081 tgagcggctg ctgattgtcg agacattcct gagcttgttg gtttagaagt ttaaggaagt
    22141 atccgtggtt gtaggcgtgg gtgagactgt cgagccgtgc ctgttcttcg gcaatggcgt
    22201 gatggtgggc gttatccagt gcgagggcgg cgcgttgggc aatggtgaag agcagttcca
    22261 catcgccgcg gtcgaaggcg ttgggttgat aagaggcaat ggccatgatg ccatcaacat
    22321 gtgtgccgcg catgggcacg cccacccagg acagcgatgt ttgattctgg ccaacgagaa
    22381 caacatctac gccttccagt acgattttct ggcgcaggtc gggcaggaag agttcgcgct
    22441 gattatcgat aacccagctt gaaaaagtac cccttctggg tagttttaca ttgtgaaagt
    22501 attccccgcc atcgtaaagc agttcaagcc gtatttcttc cccttccaca acaccgatga
    22561 aatacgaatc ggcttcgagt gcattttgaa aggcggcatt gagcaaagtg aatacctgtt
    22621 tcgtgtccaa agtagagacg atctgtttgc taagctcatt gatgatttca agtttgttga
    22681 tctgttcacg ggccattttt tttaattgct gaatggtctc gatcaccatg aatgcagcaa
    22741 tgacctggct tccgtggatg acccagtcgt aggtttccaa tttggtgttg aatcttgaga
    22801 tgatgtaaat actggtaaag ctgatgacga gactgtaagc gggtgcgcgg gtggagatca
    22861 gggatgtgga gagggaggcg ataaagacca gtgcataaag catgtggtca agttcgtcag
    22921 ggatgacgaa tgccagcgtg ctcagtgtgg cactggaaat aatgacattg gaccaggtaa
    22981 atctggattt gttcaccgaa gctgaaaaca ggatgtagta gaaggcaagg taaatgctgc
    23041 ctacaatgcc gaaaaccaaa ataacattcc ttgtgaatgc ctgttgcggc gggaagtaga
    23101 caaggcttac actcacagtg gtcaccagca ccaaaatgcc cgccacaatg gatggcaaaa
    23161 tggagatcag gttttcttgc ggtgtaaaac tactaaactt tgttttcatg ctttaatcaa
    23221 ttaataagtt gggttggttt tccttgatca gtgtttcgaa aatctcaata atattggggt
    23281 cgaacagaat gcccgattgt gagcgcaggt aatccagggc ttcttcggtg ctcatggttc
    23341 tttctctata aggacgttta ctggtgaggg catcaaatgc atccacaatt gcaaacatgc
    23401 gtgcaaggat ggggatctcc tcgccgtata gggcatctgg ataccctgtg ccatcccagc
    23461 gttcctggtg gtgacggatg agggggatgg tgtcttgtag aaatgggatt cccgccacga
    23521 tttttgcgcc gatatcgggg tggagtttca tgatcttcca ctcttcttcg ttcaacgggc
    23581 caggcttgtg caggatcgta tcgctgatac cgatcttgcc aatgtcatgc agaagcgatc
    23641 cacgttccaa caccttgagc tggtcgtgcg aaaaattaag agccttgccc aatttggctg
    23701 ccaggtgact gacgcgcaaa ctatgtcctt cggtttccct gtcacgggcg tccaacgcag
    23761 acattagtgc ggcaagggtt tggtcatatg tagattcgag catgtcgtac gcggctttga
    23821 tctcaactgc ctgccgctgt gattcaacca atagtagaga ccgttcaagc gcaattgctg
    23881 tctggctgac cagagcctgc aggtcattgg ggttggcggc atgtttgttt ccctggtttt
    23941 cgggcatatc catccataca gcgccataag tgcgaattgg agtttggatg gggaggcagg
    24001 cgcggatgat ggattgtcca cgtgaaagat agatcagctg ccccgaaacc attacatctc
    24061 tgatgatttc catgggatgg tctgttccac catgaatacc ctcggatgag atttccacct
    24121 ctgcgaccat gtcaccctct gcattgaaca ggataacccc cgtagtgatg ctcttggcca
    24181 gtttatgggc cgtttgcgca atgtcccgcg cagcatcttt cagattatcc acctgaatga
    24241 tcttgttgct taggtgatac agcagggagg tggtcctcag ggaagtgcgt agttcctgtt
    24301 gcagccatgc actttcaaat gcgcctgccg cctgaatgga aagcaattcc gccaacgatt
    24361 catcattctc gttgaaaaag acttcatttt gtttgccaaa agccagaatg accccgatac
    24421 tcaaccgatg ccagcggatc ggaatggcca gcatgcttcg gaaaacagga ttatcgatca
    24481 taagatgtga tgtggaggaa tacttgcgga catccctcac acggaaaggc tgtactgcgt
    24541 tgaaggccat tttgatcaac gcctcagaat ccaaagattc ttgcaaagac cccaaaagac
    24601 ggggagcttc tccggaataa gcgctttcgg tgaagttccg ttcctgtccc tgttggatct
    24661 gtacaaatgt aaacctggct tgcaggatct cccgcgcaat ggttgcaatc tcgcgtgtgg
    24721 ttgcagcggg ctccaccatg gcagagagct gacttcccgc ttggaccaga ttcatcaggc
    24781 gttggtggta gcttgagcgt tcccatgcgc ggctcaactc agctgccaca gcttcggcca
    24841 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcaagttga ttttcaagct
    24961 gctggcttaa ttccaataac agagtgagtt gtgcgttgcg ctttttttcg ctctttacct
    25021 gctgggttag cacaccgatc tggtcggtct gtgccaataa acggatacgc accccttcct
    25081 gaatattaat aaaaggttct atatagtgtg ccaggcgggt attccaaaga cttgctttgg
    25141 tttcggattg tacgtcctca tcatcatcct tatccaattc catcaacttg ctcatgatgc
    25201 cattgaccgt taccagaatg aggaacaatg caaaatatac cgcgatggat atcagaataa
    25261 agaaaagaaa tgcgccctcg tgggttgcag cgaatatcga gtaattttcc ccggcaaaat
    25321 tgtcaatgac gataaaaagc gcgccgagga aaataagcat ggccatttcc agcgtgctga
    25381 taatcatgac aaccttgcgc cccagaacat tgtttctggc aaccgcaagt ttattccacg
    25441 ggatgaaacc ctcaaatagc acaaccatgc acagcatggc aggtgcgatc aggttcgcaa
    25501 caagttttgg cggtgtaaaa attgcacacc aaataagcca tggaatgact aacaggcgcg
    25561 ataccaatgg gttgaatttg tgattcctgg aaagtgtaaa aaacatccag gtcactggga
    25621 ataacatcaa catggccgcc agtggaaatc tgtatggcgt cagattgatg ccagcatatt
    25681 gatccccgtt gagtatcaga tataacccgc cgaaaaaacc gattaaagca acacaggttt
    25741 gcagcaggtt gaattttata aagcgatctc gcaacggggt tgcaagtatc gcttgtgttg
    25801 ttgaaatcag tatgaggata atggcatcta taaaataatc cgccctgatc gcaagaaacg
    25861 caaacaggaa aaggaaaaga atattcaacc atcggatcat aatggccggt ttgtgtgaag
    25921 accgaaaagt tgaagtgatt tgaagcgata aagcaagggc aaaggccata atgcccataa
    25981 tcacactaac ccaagtagac atccccgcaa cgaatcggtt taagtgtgag ggaatcaaca
    26041 ataaacccac tccgaatatc ccaatgattc cagaagtgag caagtccaac tcatgttcag
    26101 tggattgctc tgtggtttgt gtgaataaca taacagggtt gattttacct acctttcaaa
    26161 agttgtggaa tggaaaaatg ttggcatgtt tttcaatatt cccctgcaat aaagccaact
    26221 tcaaggtctg atataatctt aatgtacatt tggcttgccg atggggatga gccttttaca
    26281 aaaaacttac acggacatac acacatgcaa aatttcatca ctcttgaaca gatcgattcg
    26341 gtcgcacagg cgatcaagaa acaaatttcc atccaaccca ttgtgggaat tattctcggc
    26401 tcaggtctta acggtttggc tgattcagtc cgagatgcag tgtacatccc ttataaggat
    26461 cttcccaatt ttcccgtttc cacggtccac ggacatgcgg gacggttcgt cattggtgaa
    26521 ttggaaggca aacctgtctt cgtcatgcag ggacgcattc actattacga aggctacacc
    26581 atggggcagg ctaccctgcc tgtgcgtgtg atgcagcgtc tgggcattcc cagcatgatc
    26641 gtaacgaacg ctgcaggtgg tgttcacccc gatttcgaac cgggtgatgt gatgcttatt
    26701 acagaccagc ttaacctgat gggaatgtct ggcttaaatc cattgatggg cccaaatctt
    26761 gatgagatcg gtccgcgctt tcctgatatg agtcagccat atgaccgtgc ctattgtgat
    26821 ctggcccgtg cagtggcaaa ggaaaatgga cttactttgc gtgaaggcgt ttatgcaggt
    26881 ttgagcggtc cctcgtttga atcacctgct gatttacgct tcctgcgtct ggctggggcc
    26941 gatgcggtgg gcatgtccac cgtccccgag gtgatcattg cacgacatgg caacatgcgt
    27001 gtgttgggtc tctcgggcat cagtaataaa gccaatctgg atggttccac aatcaccacc
    27061 catgaagagg tgattgaagc cgggcaggtg attacgccga aggttgaaaa gatcattcgc
    27121 ggcgtgttgc gcagtttgta gcaagagttt gatttatggc agaaatatat aatttcctcg
    27181 cctcttatga ggcgttgatt tatattgttt tggcgatagg aggtttattc tcctttcgct
    27241 ggttatggcg ttcatggcgg gaatcgcaaa ctgcggtgtt tacaatggaa cgtgagtttt
    27301 cctcccgcag gctgggacaa gccgcggcca tttccacgat cattgctgtt ttgttttgtg
    27361 ctgagttctt tgttgccact tttatcattc cagggcttcc ttcaactgct ctgatcccca
    27421 cctccaccct ggatctgatc tccacaccca caggcactct ttcagccgaa gccatgaccc
    27481 aatttgcaaa cctgcctccc caacccgcat cggtcagttt aacgggctgt acccccaacc
    27541 aaatcatgct tacctctcca actccggggc aggaagtgca gggtaccatc acattggtgg
    27601 ggacggtgaa cattcccaac tttggctttt acaaatatga agttgcccca caaggtggca
    27661 gcacttgggc caccatttct gctggtcgtg agaccgtggt gaatggcaaa ctgggtgagt
    27721 ggatcaccaa tgccctcacg cccggagact atcaactaag actcgttgta actgataatc
    27781 aaggagtatc gctgcctgcc tgtgttgtgc cggtccgcgt gatgccttga tggaaaaaac
    27841 tgtattctgg aaaaaatatg tctgagattc aaattcgtcc aaccattgcc aatgaccttt
    27901 cccgtttgat ggggtttgac catactgcca ccagtgactc ggtatggcag cttgaattgc
    27961 gccgtgatac cgggcaagtg ttggcctcgt tccgtgaagt gcgcttgccc cgatccattt
    28021 ctgtggctta cccccgtaat tcttttggtt tggccgatga ttgggccaaa cggtccatga
    28081 tgtacacagc ctgtatcggc gcggacccgg tggggtatat cagcctgctt gagcgtggca
    28141 tggattccat ggtgtgggtg accgatctgg tggtggatgt ggcacaccgt cgccaacggg
    28201 tggggagtac ccttttggcg gccgcgcagg attgggcgtc gggaagatcg taccgtcgcc
    28261 ttgggttgga gatgcagtcg aaaaacctgc ctgccattcg tcttgctcaa aagttcgggt
    28321 atgaattctg cgggtataat gaccattatt acttaacaca ggatgtggcc ttgttctttg
    28381 gcaaaacatt gaaatagtga tccaggccca aaaaatgggc caaaggcttg cgaattattt
    28441 gttggaaggg ttacatgatt aacggttgga tggaaccatt actggcgggg atccaggcgc
    28501 ttaaccagat tctgacggcg ggaattgcca ttacggcgtt ttcgctgttt ctatattcgc
    28561 tttcgttcaa cctgcgtgac agggtggcgc ggtcgtttgc gatcattttg ctttgtgtgg
    28621 tgatcgtttt caccgccgag gcgctgcagg atgaagcgat gtcgtcagaa acggttgatc
    28681 tgcttttgcg cttgcaatgg ttcggcattg tctttctccc tgctgcatat cttcatcttt
    28741 cagatgccct gcttgtaacc gctggtcgtc cctcccgtgg gcgcagacgg cttgcagtac
    28801 gcggcatgta tgttgtttcg gcttttttcc ttgctttact tgcattcgga tatttactcg
    28861 gcccgctcgt tccagatggg aagcccgccc cgcaccttca acgcacaatg tggacggagg
    28921 tcttcacgtt gttctacgtc acggccatgg tctgggcagg tgtaaatttt gcacgagctt
    28981 acagcctgat gttgacccgt tcggggcgcc gccgtatgtt gtacctgatg gctggcgcaa
    29041 ccgcacctgc gctcggctct tatccttacc tgttgtttgg atatggcctc gcggcggagc
    29101 atccctacct gttttggagc gtggcgacgg tcatcaacgt gctggtgggg gcactggtca
    29161 ttattatggc gtatgcggtg gctttcttcg gcgtttcgtg gccagaccgt gttattaaaa
    29221 gtcgtttgtt caaatggatc atgcgtggtc ctgtaactgc atccgctgct ttggcattga
    29281 tgacggtgat tcgccgcagt ggggaactgc ttggttcacc ctataatccc tttgtgcctt
    29341 tcatcgtggt cgtgaccatt ctttcgcttg aacatgccat cacatttgcc gcacctcttt
    29401 gggaacgctg gcttttcttc ggcagagatc gcggtgaact gcaattgctt caaaattttg
    29461 aggaacgcct gcttactcaa agcgatttac agcaattcct tgaatcggtg ctagccgcag
    29521 tgcgtgacca tatgcaatct ccatccgctt ttgtggcagc gctggatgat gaaaccttgt
    29581 cacccattgt ggtggctggg aatcgcgcca tgctcgatca ggaaggactc accgaggctt
    29641 tggaacagat cggcgaggac gaacgccatg agtttcagtg gggcagtttt tggattttgc
    29701 cattacgcca tcgccgccgc cctgaaatgg atcaggatga aatgccgcct ttgttggggt
    29761 tgttgggagt tgccaaacgc gataagcagt tgatcgaacg cgaccaacgc gatgccctct
    29821 ggctgttggc agaccgtgcc gcacttgcat tgcaagaccg tcaacttcag caacgggctt
    29881 tccaatcgtt ggtggatttg cagccccagg tggaaatgat ccagcgtatg cgtgccgcgg
    29941 ttcgctatga tacgcgtgcc aatctgctca cagaaccgct acccgaagaa gcagacctcg
    30001 ccaactgggt aaaggatgcc ctaacccatt attggggcgg ccccaaattg acgaataacc
    30061 cattgatcaa gttacaggtc gtgcgtgagc tggctgaaaa agacggcggt aattctgcca
    30121 atgcagtgcg taccctgttg agaagagccg tggatcaggt caaacccaag ggtgatcgta
    30181 aattcaccac agaatggatc ctgttcaata tccttgaaat gaaattcatt gaagggcgca
    30241 aggtgcgtga cgtggcttca cgccttgcca tgtctgaggc agatctgtac cgcaagcagc
    30301 gtattgctgt tgaggcggtt gcaaaagcga ttcttgaaat ggaaatgagc cgaaaagaag
    30361 cttgattttg caattcgagc gtatgcttgg gaagcattgc cgctcatatt tattgtgttg
    30421 ggaaggagtt catgatgtct gtacagaaaa acaaacatga aaatccgatg cccgtcatcg
    30481 acgagggctg gtggtcatcg gtgctgatgg aggagagccg ttattcttcg gcgcatactc
    30541 cggttcgttc ggcggggaac aggtccgagg tccgtagtga ttcgaagtct gtcgcttcac
    30601 aaaatgatgc agcgatggat gatccaacgg agaaaaaacc tgcgaccaat tggcgtcagg
    30661 tcaaggaact ttatatgagt gaccagatcg tggatctgat ggtcaccggg cacaatcgcg
    30721 gtggtctgct cgtggagggc ggcggattgt acggatttgt gccattttcg cacctgatcg
    30781 aactggcggg gaaagtggaa aacattgacc gtgaccacga tctggaagcc tatgtagggc
    30841 gctcgttgca tttgaaagtg atcgaatgtg tgccggaaga tgatcgtgtg gtcttttcag
    30901 aacgcgcggc acaatcagaa ccgggcaagc gagccgaact tttcagcgca ttgcaggcag
    30961 ggcagaaggt gaaagggacc gttaccaacg tcaccgattt tggagttttt gtagacctcg
    31021 gcggtgtgga agggttgatc catatttctg aattatcctg ggggcgtgtt tctcaccccg
    31081 gcatgattac aaagatcgga aattcgattg atgtgcaagt gctcgacctt gcccctgaac
    31141 gctgccgtgt tgccttgagc cttaaacgtt tgcaaaacaa tccctgggag agcgccaacg
    31201 ctgaattccc agaaggtagc gtccagaatg ccacgatcac caacgtgttg tcctttggtg
    31261 cctttgcacg gcttgatgcc ggtgtggaag gccttattca cgtttctgaa attccgcagg
    31321 ccaacggcag gccgttgaag gaaatccttt ctgaaggcca gcctgtgcag gtgcgtattt
    31381 tgcatcttga ctccgcccat cagcgcatgg gtttaagtat gaggcttgaa taaaaccgca
    31441 tgaccgattc atctcagcca cgcaatagac agtaccgtca ggcagaacca ccccccatcg
    31501 cgcagaacga ctggctcgat cggttcgctc gtttttacct gcaatttggc cgctttgccc
    31561 gcgacattct tggtgtggtg ttatttgctt ttgcgctcat gtctttgctg gctgtgtttg
    31621 aatccatttt caaccagatc cgcagcggca gtgaaacggg ttacaacacc gtcatgggtg
    31681 gggtcatcct ttcatttgta gccagccgtt tgcggctttg gcttggagtg ggcagcatct
    31741 tcgttattct tgggtttgga tacatgggat attggctgat ccaacgcgac aggaatgaga
    31801 tcgcctgggg cagacttatc tccctggaac tcgcctctct cctgaccctt ggcctgtttg
    31861 ctgttctcgg aaacaatgac atcatcgctg cagaagcagg ctgggatggc ggccgccttg
    31921 gctggggact tgtcacgctc gcctggcgtt tgatgggaaa gatcggcggt tccctggtcc
    31981 tgtttctgtt atggattttc accatcatca cgggttttgg gttgtggaca aagatcgaag
    32041 catggctttt gaaactggca ggagaagctc cccctgttgt ggtgcaaacg cccatcccca
    32101 gtgaacctgt ggaacagccc gtggaagaac ctgaaaagcc agctgcacca aagaagaagg
    32161 cgccggtcat ccctgctgaa ttccgaacct cgctgaaggc tgttgacaag aaagacgaaa
    32221 aacctgccaa accgcgtccg cgtaacgaag accttccgcc gctgcatatt cttctggctg
    32281 aatcagccct ccgtgcggat gaacgtacca ttaatcaaac ggcggggctc atcatgaaaa
    32341 ccctcgccga tttcggcatc cctgccaccg tgatcgggta tcgcgtgggc ccatcggtca
    32401 cccagtttgc agttcaaccg ggtttcatta aaaagcccgg caccgatgaa gaagatgtac
    32461 agcaaatgaa ggtgcgcgtg gcgcagatcg cctcgcttga aaaagatata gctctggcat
    32521 tgtctgccca gcgtttgcgt attgaagcgc ctgtgcccgg caaaccctat gtaggcatcg
    32581 aggtacccaa tactcacagc tcggttgtgc gtttgaagac cctgctcgaa tcggaggcgt
    32641 tcaacaaagt gggcgcacca ctgacggtgg ctgtgggccg tgacgtttcg ggaaatccgc
    32701 tgattgccga cctttcacgc atgccgcact tgctgattgc aggcgccacc ggctctggta
    32761 aatcggtctg catcacttcc attgcggcct gcctcgccat gaacaacacc cccgaagatt
    32821 tgcgcatggt catgatcgat tcaaagatgg tggaattgat ccgcttcaat ggcttgccgc
    32881 atttgtatgg caaggtggaa accaaccttg aacgcatttt gggtgtgttg cgctgggtcg
    32941 tcgttgaaat ggagcatcgc taccgcctgc tcgaatcggc ccatgcccgc gatctggata
    33001 cctacaatcg caaattgact cgcaaagctg atggcaaacc cctgccgcgc atcgtcgtaa
    33061 tgatcgacga acttgccgat ctgatgatgt ctgccccgga tgtcaccgaa cataaccttg
    33121 tacgccttgc gcaaatggcg cgtgcgacgg gtattcacct cgttgttgcc actcagcgcc
    33181 cctccaccga tgttgtgaca ggtctcatca aagccaactt cccggcgcgt cttgcatttg
    33241 ctgtagcctc cggcacagac agtcgcgtca tcctcgatta caccggcgca gaatccctgc
    33301 ttgggcgtgg tgacatgctt ttcctcaacc ctgaggtggg caaccccgtc cgcgcacaag
    33361 gggtcatgat caccgacatg gaaatcgagc gcatcatctc tcactggcag aagaacggtg
    33421 acgggtctga agagactcca ccctgggaaa aactcctgca ggaacctggc gaagacgaag
    33481 atgacggtct ggtagagcag gccatttcca tcgtgcgcca aagtcaacgt gcctcagcct
    33541 ccctgcttca gcgccgattg cgaatcggat atccgcgtgc tgcacgcctg ctcgatcagc
    33601 ttgaagagat gggcgttgtc ggtccatcac agggcggcgg caaggaacgt gatgtgcttg
    33661 tcagcgaaga agatttggaa gagggcaggc aggaggatct gggtaagtga aaccaacttt
    33721 cacagaccag ttgcgccgta ttttcaaagg catcctggat ccaacgggtg ccttcctaaa
    33781 tcgcagcggg ttgacgccga atgccatcac tctgttgggg ttggcgggaa ccacggtggg
    33841 tgcctacatc ctgtcacagg gatacatgac cataggcggc atcgtcctct ttgtctctgt
    33901 tctggtggat gcccttgatg gcaccatggc tcgtttgcgc ggtgaaccaa gcgattttgg
    33961 cggctttgta gattctgtca gtgaccgcta tgcagagttc atcacctttg gcgggctttt
    34021 gtattttttt ctcacacagg ccgattaccc cggtgtagtg gcaacctttt tagccacagc
    34081 aggttcggtg ctggtttctt atgtcaaagc tcgtgctgag gggctgggct ttaccgccaa
    34141 ggtcggcctg cttacccggg tggaacggta tattgtttta attcctttgc tgatcttcaa
    34201 tcaacctttt cttgcggtgg tattcattgc cgtgcttggc aacatcaccg cattgcagcg
    34261 cattttgcat gtccgcgcgc aggggcgtga acgaatgaaa aaagccaaag agaacaaaga
    34321 gtacctgcac taacatgacc gaaggtctat cgcttggccc catccttctg cgctggaacg
    34381 gcctcctgat cgcttgcggg atggcattgg gtgccctgct ttcggcacgt gaagcaaaac
    34441 ggcatggtta tgatgcggat ctcatttatt atctttttct gccgctggca gtttggggga
    34501 ctctgggagc gcggttctgg catattttga ccccgcctct ttcttccgtt cagctgggtc
    34561 tgacgacgca gcattatctc acccatgcat tggatttcat cgccttttgg ctcggtggtt
    34621 tcggcttccc cggtgcgctg atcggcggtg gattggcctt gttatatttt gccaaaaaga
    34681 atgaactctc tttctgggcg ctgaccgatg tgctggctcc ggggcttgcc ttggggcagg
    34741 ccattggacg tatcggcaac tacttcaatc aggagttgta cggtctcccc accgatctgc
    34801 cgtggaagat ctttattgac ccagcctatc ggttaagcgg atttgaagcg gtgaattttt
    34861 atcatccgct gtttgcgtat gaatcgcttt tgagcctggg taatttgttc ttgctgctct
    34921 ggctttcgcg gcgatttgct gaaacattgg ccgtcggtga cctgtttttt gcatatcttg
    34981 gttttcacag ccttgcccgc tttttgctgg aatttctacg cctcgatgtt gcattggtaa
    35041 acgggttgaa tatcaatcag gttttctttg cggtgacatt tctcttttca agtgtgagcc
    35101 tgttttggca tcatcgccgt gtccgggaat tgtaaggtct ctgtgaaggc aggccttgta
    35161 aagtggggaa tacaataacg tcaataccag tgaaggaaaa cctatgatcg aaactcatga
    35221 cctaagcaag cagttccacg attttatggc tgtggatggc gtaaatctct ctgtagattc
    35281 tgggcagatc cttgcgttgt tggggcaaaa tggcgcgggc aaaaccacca ccgtccgcat
    35341 gttgaccgcc ttgctaaacc cgacgcgtgg ttgggcgcgg gtggcagggt atgacgttgt
    35401 gaaaaatgga catgcggttc gttcttcagt gggggtgctg accgaacaac acggcctgta
    35461 catgcgtatg aatgccattg aatacctcga ttattttggt cgtatctata acctggaacc
    35521 cgcgatacgg aaaacccgca gcgacaattt gcttgaatat tacgggctga cggaagccgc
    35581 aaagcgcagg atcggcgaat actcgaaagg tatgcgccag aaactggcgc tggctcgtgc
    35641 catgatgcat gatcctggcg tattgcttct tgatgaaccc acttcagcca tggaccctga
    35701 gtcggcgcgg ctggtgcggg atgaaattgc gcggctgaaa tcatcccagc ggacgatcgt
    35761 cctttgttcg cacaacctca ccgaggtgga gtcactagct gataagatcg cgatcattta
    35821 tcgcggcaag atcctcttgc agggttcatt ggaagagatg aaacgcgatg tgctgggggc
    35881 acccgaatat gagatcagat tcagtgaggt ttgggatgcg ggtgggttgg tgctgcctga
    35941 aggggtgacc ctgctttctc ggacagcgac cagtttgaag gtccgtgtgg aacggccgca
    36001 ggtttccaat ccgatcatgt tgcaggcgtt gtctgcgctt ggagcacctt tgctggcatt
    36061 tcaagagcag gcgcgtacgc tggagcaggt gtatttgaag gtgatggcag atgccaaagg
    36121 aagtgaccat gttcaataaa ctgagaccgg tctggctggt ggcagggcgt gagttgaaag
    36181 atcaattccg cgattggcgt gtgctgacgc cgatgatcat tcttgtgatc gcattccctt
    36241 tcctgatgaa tgaattcgca aaacagacgg ttgattttct gaatcaatac gaagcgaatt
    36301 tgatcctcga caggcttgtg ccattttcga tcatgatcat tggtttcttt ccgatcacca
    36361 tttcgttggt ggtggcattg gaagcctttg tgggtgaaaa ggaacgcggc accattgagc
    36421 ctgtgttgag cgcgccgctt gatgattggc agttgtattt tggaaaattg ctggtgggtg
    36481 tagccacacc tctgacggcg agttaccttt cgattgccct gtacctggta atggttgtcc
    36541 gtcagggact tggaatccct tcgctttcgg tgatggttca attgttgttt ttgacgaccg
    36601 cacatgccac tttgatggtg agtgcggcga tcgtgatttc ggtgcaatcc acctcggtga
    36661 aggctgcgaa tttgctggcc tcgtttattg tgatccctgt tgcgattctg atgcagggtg
    36721 aggcggtgat gttgttttgg ggaaatgaag acgtgctctg gctggccgtt gcaggtgtga
    36781 tgatcatttc gctgctattg atccgcgtgg gtattgcgca ttttcagcgg gaatacctgc
    36841 ttggccgtga aattgatacg atcaacctgc gctggatttg gggcacattt tggaagaatt
    36901 ttttcggcgg ggcacattcg cttcgtgaat ggtatggaat gtcagtgggc ggctcgttgc
    36961 ggcgtgtttc ttccgctgtg ctggtggtga ccttgatcgc cattgtcagc atatggcttg
    37021 gctacgattg gatgatggtc aactttgcgc ctgtcattga acatgccacc cctgaaaggc
    37081 tgcaaaaaat tacgaacagc gcacaggatt tttcaggcct gacggatttg catagaacca
    37141 tcactgcgcc gttccttttt gccaacaata cccgcgcagt ggcggtcatt tttctggcgg
    37201 ggttggtttc gttcagcgtt ttgggggtgt tgctttacat ggttaacatt ggcttgatcg
    37261 gcggattgta tgcgttgttg aagttgcttg gcatttcacc gttgcccgtc tttgccgctg
    37321 gtattctgcc gcatgggatc tttgaattgc ccgctgtgat gatcgggtct gcggcgatgt
    37381 tgtacatggg cgtggctctt gttacgcctc aaacaggcaa atcgatgggt gaggtgatca
    37441 tcgagctatt tgccgactgg ctgaagatct tcctcgggct ggttgttcca ttgctggcca
    37501 ttgctgcggt cattgaggcg tatgtcaccc ccgttcttct ggtgaatgtg atgagtaagt
    37561 gagttgctga aaaaggctag acccgaagtg gttttgcatt aagtcttcgt tgtaaaaatt
    37621 gattttcaga tcaggcaaag ttggaaaggc gctttttgtg cttttcggct ttgcctgatt
    37681 atgtgaaaag gactggaaaa tctgccgtca agcgatacaa tttgagaagg ttcggttcga
    37741 attttcgaaa taaaattgtg attcacgaag gcaggaatgt aggataagat aaccatatgc
    37801 caaaagtaat catccttggt tcgtcgaatg cgatccctac aaaagacagt gaaaatacac
    37861 acatggtgtt ggtcggcagg gagcggatgg tgttgatcga ttccggcagc aacccgattt
    37921 tgcgacttga gcaggctggg ctggatttta atgacctcac cgatctaatt ctgacccatt
    37981 tccaccctga ccatgcttcg ggcattccat tgcttttgat ggatatgtgg ttattggggc
    38041 gtgaagctcc gctcaatatt tatggattgc actacacatt ggatcgtctt gagagcttga
    38101 tgggttttta caactggtct gattggccga attttttccc agtcatcttc catcgcctgc
    38161 cgctcaagga aatgaccccg gtcttgaatt gttccgattt tacgatccat gcctcgcctg
    38221 tgcggcattt cattccaaac attggcctgc gggtggaatt tgcccaaagc cagaaggtga
    38281 tggcgtattc ctgcgatact gagccctgtg acgaggttgt gcgtctgagt gaaaatgccg
    38341 atgtgttgat tcatgaagcc acaggtgaaa ctccgggtca ttcttctgca aggcaggcgg
    38401 gtgaggtggc aaagaaggca ggtgcaggca ggctgtatct catccactat cccagcgggc
    38461 gttatgcgag cggcgacccg gttgcagagg ccggtgaaaa tttccgcggc acagtagccc
    38521 tgatgaaaga tttcatggtc ttgaaatttg gtaaaagact accgctctcc catttcggaa
    38581 gagcggcaga aaacagcaaa aacagttcag gtaagaaggc gggaaaaatc taccagcccg
    38641 aaatataagg cgcccactcg ccattctcgg tcagatgcct cccaaagatg taggcggcat
    38701 tggtgggtgg ttccttggga atatgcagga ggtgcatgtg ggcttcctcc actttgcggc
    38761 cgcctttgcg gtgattacag gcagagcagg ccgccaccag gtttgtccaa atatgctggc
    38821 cacccatgtg gcgtggaatg acatgatcca cagtaagagg cccatctcgt ttcccgcaat
    38881 actgacaggt ataattatcg cgacggaata tttcgcggcg cgttaattta acgtgagggc
    38941 gcggacgatg aacttgtgat tccaaccgaa tgaccgaagg gcggggaagc atctgagaga
    39001 ttgtgcggat gtacccgcgt ccattgacga tcaaaccggc tttccctgca agtatcagtc
    39061 caacagctct gcgtgttgag caaatatgga tcggctcaaa attagcgttg agtaccagca
    39121 ccggttcgta cattttgcct cggtaatctg catgattata ctgccactta aatgaatccg
    39181 cgcggtggcg cattacagga gcatttgaaa tgaacatcat gatcgcgggc ggctcgggtt
    39241 ttttgggtaa cgccctcgca aaatcctttc ttatggatgg gcacaaagtc tttattctca
    39301 cccgtggttc gcgcaccatg aacggaacac aggcgatcca ctgggatgca aaaacgacag
    39361 acggctgggg gcattgggtc aacgagatgg atgtggtgat tcacctcgcc gggaaaacgc
    39421 ttgcctcctg gccgtggacc aacgccatga aaaaagcctt tcatgactct cgcatcattc
    39481 ccggactggc gcttgttgaa gcaatccaaa aagcgactca gcgcccgggc atttttattc
    39541 agcagtcggg catcaattat tatggcctga gcggcgatct cgcagacgaa tccacgccgc
    39601 ccggggatga tttcctttcg cagcttgcgg tgcaatggga aaatgccacg aagccactcg
    39661 aagagttggg attgcggcgg gtgatccttc gcacggcgcc agtgctcgat aaaaaagaag
    39721 ggctcatgcc gttgatggct ctgcctgtta aattatttgt gggtggcccc atgggcggtg
    39781 gaaaattttc cgcgccgtgg atccatgttc aagactgggt tggagccgtg cggcatttga
    39841 tcgcaaatga agatgcccgt ggggcataca acctgatcgc gcccgagccg acctcaaacg
    39901 cagaattcaa taaaacgctt gccgatgtgc ttcaccgtcc gtattggttt cctgtccctg
    39961 catttttact gaggaccttt ttgggggaaa tgagcgtctt ggtggtggaa ggcaggtttt
    40021 cccaacccaa acggctgatc gagtcagggt accggtacca gtttccaggt ccgcgtgaag
    40081 cgatgaccga tttatttagt taatgtcaaa ggagagaaat ccatgaaaaa aatgatcctt
    40141 gtgctggcag tcctcctgac tgcctgtggt acttcttcac aacagcaccc tgtggtggtt
    40201 atgccagcat taatggccac ggcagaaccg tatattgatc cttcataccc aactgcgcaa
    40261 gtggaagtgg ctgcgccgaa ccaaactgcc agcggcattg aagtgcgtat ggaccgggtt
    40321 tcgatggagg gaaaaaatat caatgctgat gtttgtttta cccttcccga tactgctgat
    40381 tggggcattc agtcggcgag cctcacatat gggggagtgg tggtgcaaga gtttggcaca
    40441 accctgctga gttttcagga gcccgtcaat ggggcggtgg gattgcgctg tgatacgttg
    40501 acttttgtgg tgccgcccga tgccgacctg accaatgcgg cgatcatcat tgatgcgatc
    40561 ggcaccaccg tgcgcgccga tgattactgc tccatttaca tgcccaagat ccaacaggcc
    40621 atgctcgacc gtggcaccgg tatttcagtg gattgcatgg atgtgaacgg aatgcttacc
    40681 atgcagatcc tcagcatgcc ccccggaatg acccaggagc aggccgaatc cattgtgtac
    40741 agcgatgagt tctacaccgt caaaggcccg tggaccttcc cgtttggttt ggcacagtaa
    40801 aattcttgat gtataatacg cgcatgtcca gttccagttc gttcaacaaa tattattatt
    40861 acacccatcc ataatccctg cgatggagtg ctttgaacgc gcccacatca tcgcgggcgc
    40921 gtttttgttt gtcaattcac cgtaatgtac ggttaaggta tgaaggagaa gttatgaaca
    40981 tcgaagaaca ggttgagtta ttgatgctcg gcaccgaata tggcgatcag gacttgaaga
    41041 aggccatgac cgctgaattg cgcgagcggc tgacccttgc ccaaaaagag aacaggccgt
    41101 tgcgtgtgta ctgtggttac gacccaacat ccactgatct gcatttggga cataccatca
    41161 ccatgcgcaa attgcgccag tttcaagacc tgggacatga agtcactttt ctgatcggga
    41221 gctatactgc tctggtgggt gacccttcgg ataagaacaa ggcgcgtccc attctgaccg
    41281 aggagcaggt tgctgaaaat gcgcggacat acaccgagca ggcctttcgt gtgcttgacc
    41341 gtgaaaagac gaagatccgc tacaacggtg agtggctttc gaagctttca ctggtggatt
    41401 tgattcgtct tgggcagaac ttcactgttc agcagttttt ggcgcgtgat aattttgcca
    41461 atcgccttga aaaaggtgag ccaatttaca ttcatgagac cttttatgcg ttgatgcagg
    41521 ggtatgacgc tgttgccatg gaaaccgatg tgcagatcgg cgggtcggac cagttattca
    41581 atatcatcgt tgccgggcgc aaattgcagg aagcgctggg gcagaaaccg ttggtgggca
    41641 tcatcactgc aattctgcca ggaacagatg gtgtgcagcg gatgtcaaaa tccactggaa
    41701 atatcgtgcc gattaataca ggcgcgaacg atatgttcgg taaattgatg tctgtgcctg
    41761 attcggcgat ggggttgtac atgcgtttgg tcacacgctg gagcccgccc gagatcgaga
    41821 agatcgagca ggatgtggct tcaggagcct tgcatccacg cgatgccaag atgaatatgg
    41881 caggtgagat caccagcatt ttctatggcg aagaagacgc gaagtcggcg cgtgagaatt
    41941 tcgtcaagac cttccaacag aaggaaattc ccgatgaaat gcctgagttt tccttgcagg
    42001 caggccagac ggtggtggat gtgatccttg cggcggggct ggctgaaagc aagagcaagg
    42061 cccgctcctt gattgaccag aagggtgtga aattggatgg tgaagtgttg gaacgcggcg
    42121 atgctgtatt tccacaccct ggtgtgttgc aggtggggaa gcggcgattt gtaagggtta
    42181 agtaaattta taaaaagaga tgagccagcc agctcatctc ttttttgttc tgcttgttgg
    42241 gttctttttg agcacttttt tgggttcatt ggccgctttt gaagccgtgt cattttcgat
    42301 catgcggctg aaggcttcca caacggtcgg gtcgaattgt ttgtttgata ggtctttgat
    42361 gtatttcatg gtcttttttg gagaccatgc cttgcggtaa gggcggtctg agagtaaggc
    42421 atcccaaacg tcgatgatgg caaagattcg agcggcaagg ggaatctctt ctcctttgag
    42481 gccgcgtggg tatccgcttc catcccacca ttcatgatgg cagtaggcta tgtccactgc
    42541 gggcctgagg taggcgatgg ggtagagcag gtcataggcg tatttgggat gtttccgcat
    42601 ttcgaacagt tcgctctggg taagcgggcc gtccttgtgg agaatggcgt cagatacacc
    42661 cattttgcca atatcatgga gcagggcgcc gcgacggatg tgcaataatt cggtttcggg
    42721 aatgttcatt tgccgtgcca gttcgagggt caggtttgtg acgcggttcg tgtgtccctt
    42781 tgtttctttg tcgcgcagtt ccagcgcttt gccccagcct tccagcgttg tgtcatatgc
    42841 gagggagagt tcctgattgc tgcgctgcag gttttcgaag agttgggcat tgtcaatggc
    42901 gatgatggct tgttctgcca gtgtgtggat gaaatcaagc cagtctccgc tgggggtaaa
    42961 cgagtggcgg aaataggttt cgaggatgcc cttggtggcg cccttgctga agaggggcat
    43021 ggcataataa cttacgaatt gttcggttcg caggattttc atctgcggca ggtcaggctc
    43081 gttgttgaga tcttttatgt agatcgcccg ccgatttaag aggatttgtc ctgcaaggcc
    43141 atcgccgatg ttttgtgagc cgcgtacggc ttcacgattt ttgaatccga tgggggcgtg
    43201 gtagttcagc gtttggctgt tggggttgaa taccagtacg gtggcggcgc tgacgcccat
    43261 tttggtggtg aggtgttctg tcagaattcc gagggtaatg tgcaggtcga ggctggaggt
    43321 gatggcggta tccatttcgc gcagtgtagt gaggcggcgg atctgttcct cgcttctttc
    43381 gaaaaggttg gaacggtaaa tggcatttcc tgcaatttca gtgagtgtat tgatcaagcg
    43441 gatgtgattg gaaccgatct tgtgcggtac cgacatggca acaacaagaa cgccaatggt
    43501 ttcagagaca gttcggatcg gaagcgtgac actgctccag ccttttccga agaagctggc
    43561 attttcgggg gcgagtagga tatcgcgaga tagctcgggg ctgacgtgtg ttgcaccttt
    43621 ggaatatacc gccccgacaa ttccctcgtt cggtctgaac gtggatttgg gcaggtcttt
    43681 taatcggccg cttgtagcaa tggggacgag ttcgttcttg ccgtgatcgt agagccagat
    43741 ggcagccgag tctgtgccca tgttggcttt gatctcgttg agcaaaatgg ggatcatttc
    43801 tcttgtatcg tgtgcggtgc gcagggcaaa agagacacgg ctgaccattt cgagttcgtc
    43861 gagtctttgc agggtctcgg tgtagaggcg tgtattttcg agagaattgg ccgcctgatg
    43921 ggcaaaggtc tgggcgatga gggcatcgtt tttggtaaac gtgtttggaa tgacacgatc
    43981 aatggtgatg tagcctgtta cctgtccgcg tgtgatcatg ggaacgccca tccatccgcg
    44041 gactttctcg gcatcgcccc agcgttcgaa gcgcgggtct ttgttgcagt cgtcaataat
    44101 taggggttcg cccgtttcat tcatgatctt taatagaaca tgatcgatcg ggaatgtgtg
    44161 gtcaatggct ttttcaggga attggaatcc ctttgcggca gtgatgcgga tgtgcgctcc
    44221 ttcgatttcc ataatggagg cactgtcgta tggcacgatc ttgcgcagcc attcgaggat
    44281 ggcttcatga agggatggaa ggtcgagcag gctggagagt actgtggtgg cctgccgcat
    44341 gatctcggac tctttgcggc gtttttgttc caattcgaag aggcgggcgt tttcaagtgc
    44401 ggtcgctgcc tgattggcga aagtttgtac caaaacggcg tgttcttcgg taaatgtgcc
    44461 cactattttg ctgtcaagat ttataaagcc gatcagttta tcttgaatga acatggctac
    44521 acccatccag ctgcggatgt attcggtacc tgtccatttt tgaaaatcaa agttctgttg
    44581 ggcgtcatcc agaatatttg gcttgcggct ttcgatgatg gctttccatt tttgggtgag
    44641 cggaaagatc tgcccgatgg aaaaatgcct tgggatgttg cgcatggcgg tcattttgag
    44701 ggtgtcgttt gaattgacca tcataatgga tgcgctgtcg tagggggcaa gctgttttaa
    44761 ccaatcaagg atgttttcat ataagttata aacatccaat gtgttggcga gagcggatgt
    44821 agcctgattt agaacttctg cgttttgcag gtgtttgtgt tcggcttcga acaggcgtgc
    44881 gttttcaatg gcaactgtgg cttgttgagc gagattgttg agaaaatcga ggtcggtgga
    44941 tttgaacgcc ttgtcctccc cgacgcgcca aaccaccagc aggccggtca gctgtccctt
    45001 ggaaaggaac ggcattccca tgatgtgttc atcacgttca atttcagttc caaccaccgg
    45061 aatggagcgc gggtctgtga ttgtgttgtt gactatctca ccctgcttgt ttaaagcaat
    45121 gttgcccaga atcccatttc cgattttcaa cggatcattc ttgatctcat ctgattcacg
    45181 tccaagagct gatatggctc taagactggg tgtattgggg tcggttaaat acacggcact
    45241 agtttctgct tgcaggagtt ctttggcgta gtaggcgatg ctgtccagta ccttattgag
    45301 ctgtaaggtg gatgaaatgc tgcggccaac ctcggcgatg gcagctgctt cctgtgcgtg
    45361 acgtgtgatt tcttcaaaaa gccgtaccga ctcaatcgaa atggatgcct gatgtgcaat
    45421 gctgacaagg aagtttagtt ctgagttatc gaataagcca tttgcttttg gccgccacgc
    45481 attgatggcg ccaaaggatt ttccatgcaa ggtgagtgga gcagacatca tcgtgtcaag
    45541 gcgggcatct tctttaggag taccgggaac agtgactgta cgcgtatcct gcgaaaagtc
    45601 atttacgatt tcaggcctgc ctgtggcaat aatattccct gtgatgcctt ttccaatttc
    45661 aagggtatgt gaaagcaact gatcctgata ggcacccttg gcagttacga tctttattgt
    45721 tttttcgtca tcctgcaata ggtaaatggc aagtgtgctg gctttaagca gggaaagggc
    45781 acgttcggcg atcttatcca atgccgggat aacttctctg gtggtagcga tttcgttggc
    45841 aatatttgcc aatgtttcaa tgatctgggt gcgccttttt tcttcgttga ataggcgcgc
    45901 attttcaatg gcgatggaag tatgatttgc tactgcaagc ccaagttcct catcctgttt
    45961 ggtgaagtgg ttgagttgat agctttcgag cgtgatcatg ccaatcactt tttctttgac
    46021 aatcaacgga atacccatga atgagagcac ctgcgagttt tcgctgaaaa atttgaagcg
    46081 atcgtcaagg taaacgttgt taagtcggac tggctttttt tccgtgatga cgtaattatt
    46141 caaggggtat ttaccaagca ggagcggccc cttttttgaa aagttgagga tttcagatcc
    46201 gattccagca gaaatgacca gacggtcttt atccaggagt tgaatgctgc ctgcgtcgga
    46261 agatatgacc ttttgtaatt gatcgagaat ggattggagc acctgattta agtccaatga
    46321 ggagttggcc aggcggataa gttcccatag ggctgtttct cgctgatgct gaacttgctc
    46381 tgccttcgaa agccgcaatt tttcgattgc ggtccccaga ccgcctgcga tggtgttgag
    46441 caggcgttca tccttttcat caaatgcgtt tttgtgcctg ctttccacgt taaatacacc
    46501 gatgatacgt tcctgcactc gaaaagggat gcagatctca gattttgtgt ttgaatctat
    46561 ttcaatgtaa tccacatcct gagtgacatc tacaatgcgt atggcccttc cactccgcac
    46621 tgctctgcct gaaacgcctt gggtgactgg aaaaccgttt ttccatttgt caacgtttgc
    46681 gccgatataa gaggggtggg gagtgagtgt atcccctttt tcgttcagca agagtattcc
    46741 acatacatca gtatatatct ctgcggtgat ctgggtcact tgggcaatca tgtcgtcttc
    46801 agaattgctt tgtgtgccag ccatggtggt ggcgcgcaaa acggttaatt cttttagttg
    46861 gcgttcaagc agactttccg cctgtttgcg ggttgtaata tcctgtgcaa tggcaataat
    46921 tacatctttg ccgaagtagg tgcctttgtt gagggttacg gttttaggga atacctgtcc
    46981 attacttcgg atgccccaaa attcaaactc ttgaggagtt ccattgaaag tatccgcgat
    47041 tttttgcgca aggctttcca aatcattttt cccgggggca ctcaatatct cgggggtttt
    47101 cccaataaaa tactcccgag ggtatccata catcttcact gcgccatcgt ttacatcaag
    47161 aaatactcct tctttatcct gaatataaat ggcctgtgtt acgctgttga aaagttctcg
    47221 gtaactgatt tcattttcct tcatgacctt ttcagagttt ttttgttctg taatgtcaag
    47281 aagggttccc tgccagaata ggggagtgcc atcctccgct ttgatgatgg aggcatcttc
    47341 ctttatccat atgtagtgtc catctcgatg ccgcagccga tattcgatgc ggaagggttc
    47401 tccagttttg tcagtccgtg catcttctgc aagaactctt tctctatctt cggggtgcag
    47461 tgatttttcc cacatattta ccccggcttc ccattcccct ggtgtgtaac caagaatttc
    47521 cttcaatcgc gggctcatgt attgagtgga ttgtgggtca ttgaaattat ccataaaaat
    47581 gacggatggt aatctttcga ccagagtacg gtagcgctcc tcagcttctt ttaaagattg
    47641 ctcccccttt ttttgttgtg taatatcagt tacgaacccc tcgtagtata gaacctggtt
    47701 gtttgcgtct ttgacgacgc gggcatttgt tgatgtccag aagatgcttc catcttttcg
    47761 taaattcttc gcattgtagt tttttacata tccattcttt gtcagttctt ctgtgaactt
    47821 gcgccgacca tcagaagacg catgaatttg agaactgatg tcatggacaa gattaagcat
    47881 gtcttctggt gattcatatc catacaaggc agccattgca gaattgacgc gaagaaaacg
    47941 tccgtctggt gaagaccgga aaataccttc tgttgctgat tcaaaaagat ctgagaagtt
    48001 ttgttcagct gcttgaagtg tgtttctcct gtttttcaat aatataaaca acaggattgc
    48061 ggtaacaatg acaaaggacc accctttgta agattggata acggatagtt gatcaggatt
    48121 cgtgaagata agagttacaa gacggtcgga tagtaaaatc catgtgcccc ctataaaagc
    48181 gtatattaaa gaaatgccta atgcagagaa tggttgcttg ttcttcatgg ttctgaacag
    48241 attttttctc atatttgatt atagactttt tttcatggaa tctccatgag aaattcgcaa
    48301 caaaaaaagc aaatcagaag tgatttgctt ttgaagaggc gtcgagggga tttgaacccc
    48361 tgatgaggat tttgcagacc cttgccttac cacttggcca cgacgccatg tctatagtat
    48421 ttctctatat ctgagcgggc gatgggattc gaacccacga ccttctcctt ggcaaggaga
    48481 cgtactacca ctgtactacg cccgcgtttc ttggccaaac catcagccag agtgccgaga
    48541 cccaggatcg gactggggac accgcgattt tcagtcgcgt gctctaccaa ctgagctatc
    48601 tcggcatgaa catctttttg taaagcggtc gtgattttac acgtacttat atagattgtc
    48661 aagaaacgcg cacgaatctg ttttatgtct gtgctgatga tgatagttct tgacttttta
    48721 tattcgtttc gatataatgt taggtcgctg tccaagtggg acagttaaaa tcgtttcata
    48781 acccagccat agagttagga gagaagaatg gcatcttctt tgccccaaaa aacctacgca
    48841 cgtattccag taaaactaga tctccccaac ttaatagaag tacagcttga ttcattcgaa
    48901 cgactcaaaa aagagggttt gaatgacctg tttcatgaaa tctcacctat tgagtcctac
    48961 aacaaaggaa tgaaactgtt ttttccaagc cgaagtcctg agtctcagca atggggactc
    49021 aagtattggt ttggagaccc caaacatggg attgaggaat gtgttgagcg tgacttgacg
    49081 tacgctagcc cactatatgt ctctgtttta ttggctggcc cagatgtgcc tgaaccaatc
    49141 aaacaagata tcttccttgg tgacttccct gagatgaccg ataagggcac gttcattgta
    49201 aatggtacgg aacgtgttgt tgtatctcag ttgattcgat ctcctggcgt atatttcgag
    49261 gcgcctgctg atcgtgcaac agggcgtccg ctggcaatgg caaaattgat tccagaccgt
    49321 ggcgcttgga tggaattcga aacacgcaag tccgattaca tcatcttgaa attcaaccgc
    49381 aaacgtacag ttccaatcac cgtatttttg cgtgccctcg ccgctgttga tgatggattg
    49441 aaggactcgc ccattaagtt tggtactgat gaagaaatcc tctccatctt taaggatgta
    49501 gataacaatc ctgagcgcat gtttattgcc tcctcgctca aacaagagcc tgactggagt
    49561 cagtacaacc agacaatccc caatgcggcc ctaatcgagt ttttcaaaaa aatgcggccc
    49621 ggtgatcctg ccactcttga taacgcccgt caattccttg aagagcagtt gttcgatcaa
    49681 cgccactatg atcttgaacg tgtcggtcgt tacaagttga accaaaaact cgacctgaac
    49741 attcccatcc cacatcgtac agtaaccaag agcgatgtga ttcgtttgat tcgccgaatg
    49801 attcagatta ataatggtgc tgaacgcccc gatgatattg atcacttggg aaaccgtcgc
    49861 gtcaagacag taggggagct gattcagaac aaattacgca ttggcttgcg ccgcatggaa
    49921 cgtgttatta aagaacgcat gtccattcgt gatcaggaac agctttctcc tgtgacgctt
    49981 gtcaatatac gtcccgtagt agctgcgttg cgtgaattct ttggatcttc tcaattatca
    50041 cagtttatgg atcaaaccaa cccattggct gagttgcgtc ataagagaac tttgtctgct
    50101 ctcggcccag gtggtcttcg ccgtgaacgc gcagggtttg atgttcgtga cgtgcatcat
    50161 tctcactatg ggagaatctg cccgattgaa actccagaag gtccaaatat tggtcttatt
    50221 gggcgtttgg catcttttgc tcgtgtaaat gaatatggct tcattgagac accctatcga
    50281 agggtgtata agacctttaa ggcagatgat gaacgcttgg aagggcgtac tctgcgtgaa
    50341 gatgtatatg atccaaaaag cgaggagctt ctcttcaagt ctggaacatc aattgacgcg
    50401 aaggctttga agaagcttgc caaggttggt ggagaaatta gtgttgttcc tttcgtctct
    50461 gatgagattt cctacctttc tgcggatgct gaagataaat atacgatcgc tcaagcaaat
    50521 gctcaattga ccgaaaataa agagttttca cgcgaacgta tttcctgccg atatcactct
    50581 ggcttcttgt ttgcaggctc agaggcaatt gattatatgg atgtggcccc gcatcaggtc
    50641 gttggtatta gtgcggcgct tatcccattc cttgaacatg acgacgctaa tcgtgcgctc
    50701 atgggttcga acatgatggc tcaggcagtt cctttggttc gccctgaaat cccacttgtt
    50761 tctacaggta tggagggcca tgcggctctt gattcgggtc aggttgttgt tgctgatgca
    50821 gacggtgagg tggtctcggt aactggatcc accatcacgg tcaaagaaaa gagaagcgga
    50881 aaccgtgtat accagcttcg caagtatcag cggtctaatc aaagcacctg tatcgatcaa
    50941 cgtccttctg ttgtaaaggg acaattgatt aaggctggtg atattattgc tgattcctcg
    51001 tctaccgata gcggtcagct ggctcttggt caaaatgttg tggtcgcctt cctttcctgg
    51061 gaaggtggta actttgaaga tgctattcta ctttctgaac gtctcgttca ggatgaccgc
    51121 tttacctcag ttcatattga aaaacatgag gtggaggccc gcgataccaa acttggcccc
    51181 gaggaaatca cccgtgacat tcccaatgtt ggtgatgatg ccatcaaaga ccttgatgaa
    51241 aacggcatta ttcgcattgg ggcagaggta ggtcctaatg acatccttgt tgggaagatt
    51301 acacctaagg gcgaaaaaga acttacccct gaagagcgtt tgttgcgagc gatctttggt
    51361 gaaaagtctc gtgatgtgaa agatacttca ctacgcatgc cccatggtga gcgcggtaaa
    51421 gttgtagatg tgaaagtatt tacccgtgaa gacaattccg acctctctgc cggtgtagat
    51481 atgatggtgc gtgtttcagt tgcgcagcgt cgcaagatca cagcagggga caagatggca
    51541 ggacgtcacg gaaacaaagg tgtggtttcc aaagttgtgc ctgttgaaga catgcctttc
    51601 cttgaagatg gaacccctgt cgatcttatt ttgaacccgc tcggtgttcc tggacgtatg
    51661 aacattggtc aggtattgga agttcacttg ggatgggccg cgaaacgtat gggattccgt
    51721 gctattaccc cagtgtttga cggtgccagt gaagagcaaa ttgaagctga acttgctcgt
    51781 gcatggatca tggatcagtc ttggaaggaa gctgcggaaa aagcatggga atggcttaag
    51841 gatcaggaat atgacccgaa ttccattcaa gatgatgatg aagttcgacg cttatatctc
    51901 gagcattggc ttggtaaacg caaatatgat gtctacagct taaatgatct ggattatgcg
    51961 cgtcatgcga cagcccgtga atggttgcgc gaaaagggct ataccaaccc tgaagagatt
    52021 ctgctcgaag accgcgatgt ggccaaccct gaccctgaga tcgatgacat gactgtaaag
    52081 gcttgtctgc gtttatggat ggagtctctt ggtttgaccc gtcgtgttgc tgaagataaa
    52141 gtgtatgata cagcacagga agtttccaaa gaaaaaggag tcccacttcc gattcttggt
    52201 aagcaggtgc tgcgcgatgg caagaccggt cttccatacg atcaacccgt tactgttggt
    52261 gtgatgacta tcttgaaact ccaccacctt gtggaagata aagttcatgc ccgctctaca
    52321 ggcccataca gccttgttac tcagcagccg ctgggtggta aagcacagtt tggtggtcag
    52381 cgttttggtg aaatggaagt ttgggcgctt gaggcatacg gcgctgcaca tacccttcag
    52441 gaaatgctca ctgtcaagtc tgacgatgtt caaggccgtg taaacaccta tgaggcgatt
    52501 gtgaaaggtg agccgatcga agaaccaagc attcccgcct ccttccgtgt tcttgttaaa
    52561 gaattgcagt cactcggtct tgcagtggaa gcaattaacg aaggcggtga tgtggtcaag
    52621 ttcggtaagg atgacgagaa agcgcacccg ccaaaacacg atactggttt attgagtctc
    52681 ggagagaatc gaactggaaa aaagtagaga ttcctattga cgcaaaaggt atggcgtgat
    52741 aaactaatca gccgttgccc aatgaaagca agagtggctt tatccccact cgacccaact
    52801 ggcagctaca aatgaggcca tcagaaaaat tcgttgatgg tctcatttat tgcgaatatt
    52861 caaatgaatt tgtgaaataa tttgtaatcg gaggcggtta tgccgacagt aaatcaaatg
    52921 gtccgtaagg gccgcaaaaa taataaaaca aaaagcaagg ccccggcact tcagttcaca
    52981 ttgaacagtt tcaagcagcg ccgtgtacga caggacaaag gtgcaccgca gaaacgcggt
    53041 gtttgtactg ttgttcgtac catgacgccc aagaaaccaa actcagcgtt gcgcaagatt
    53101 gctcgtgtac gtttgaccaa tggtattgaa gtcacagctt acattcctgg cgaaggtcac
    53161 cagttgcagg agcactcagt ggtattggtg cgcggtggtc gtgtgaagga tctgccgggt
    53221 gtacgttatc acattgtccg tggttctctc gataccaccg gtgttgcgaa tcgtaagcag
    53281 ggccggtcca agtacggcgc gaagcgtcca aagtaacaat ataagatgga gtaatagagc
    53341 atgcgtagag caaaacctga aaagcgggaa atccttcccg atagccgttt taatagcgta
    53401 aatttgcaga ccttggttca gcatgtgctt aaacgtggta agaaaagcac ggctgttcgt
    53461 ttgatttatg atgcaatgga tctgatcaag gaacgcactg aaaaaaaccc catggatgtt
    53521 tttgatggtg cattgaagaa cgtcggtcca gcaatggaag ttcgtccacg ccgtgtgggt
    53581 ggtgcaacct atcaggtgcc aatggaagtt tctgctgatc gtcgcacgac tttggcaatt
    53641 cgctggcttc tcgctgctgc tcgcgatcgc tctggtaaat ctttttctga caagcttgca
    53701 tctgaattaa ttgatgcctt taatgaaact ggttccgcta ttcgtaaacg tgacgagacc
    53761 cacaagatgg ctgaagccaa tcgtgctttc tctcattacc gtgtttaatt tgatttaatc
    53821 ttaaatttgc aaaactaaat aataggaagt agttaagaat ggcacgtgta catccgctgg
    53881 aaaaatacag aaatataggc attattgccc acattgacgc tgggaagacc actaccaccg
    53941 agcgtattat gttttatacc ggtatgaccc atcgcatcgg ttctgttgac gatggtacaa
    54001 cggtaactga ctggatggtt caggaacgcg aacgtggtat caccattgtt tctgctgctg
    54061 tttccgcgga gtggaaggga tatcaagtca acattattga taccccagga catattgact
    54121 ttactgcgga agtacaacgt tcccttcgtg ttttggatgg tggggtggtt gtatttgatg
    54181 ccgtgcaagg cgttgaacct caatctgaaa ccgtgtggcg tcaggcagat cggtatggag
    54241 tacctcgcat ttgttttgtt aataaaatgg atcgcgtcgg tgcttcctat gaacgcacta
    54301 tagaaaccat tatagatcgc cttggagcca acccgattcc aatgcaaatc cccattggtt
    54361 tcgaatcaac ttttaaaggc gttgttgacc tgctttccat gaaggctatt gtttgggaag
    54421 acgatcttgg caaagaacct catgtcgttg agattccagc tgatctgaaa gatcaggctg
    54481 ttgaggcccg tgccaaaatg gttgaaaaga tcgcagaact tgatgatgat ctgaccatga
    54541 agttcttgga tgcccaggag attagcattg aagaactgaa ggttgccctt cgtaagggtg
    54601 ttctttcaac caaagctgct ccggtatttt gtggatcttc tttgaagaat aaaggtgtgc
    54661 aggttttgct ggatgcggtg gttgattacc ttccctcccc ggctgatcga cctgttatca
    54721 ctgtgtctga accgggtaac cctgacaata cctttgatat tcctgctcag gatgatgcac
    54781 ctttaggcgc tttagtgttt aagattgtaa ccgatccgta cgttggtcgc cttgcttatg
    54841 ttcgtgttta ttccggcgtt ttaaatcagg gacaaaccgt tcaaaactcc accaagaaag
    54901 gcagaaagga acgtattgga cgtttaattc gtatgcatgc tgatcatcgt gaagatgtga
    54961 cggagattcg tgcaggcgac attggtgcag ttcttggttt caaagaaagt tttactggcg
    55021 ataccctttg tgatacaaag gcgcttgttc tcgaaacaat ctctttccct gagcccgtga
    55081 tttcaattgc tgttgaacca aaaagttctg cagatcagga aaagatgggg gaggctcttc
    55141 gcaagttggc agaagaagat ccgacacttc atgttaattc agatgaagac tctggccaaa
    55201 ccattcttag aggtatgggc gaacttcatt tggatatcgt ggtagaccgc ttgcagcgtg
    55261 aatttagagt gcctgccaat gttggtgcac ctcgtgtggc gttccgcgaa tcaataacca
    55321 aaccaattaa agaagtcaat tacaaatacg cgaagcagtc aggtggccgc ggtcagtatg
    55381 gtcatgttgt attttccatg gaaccaggtg agcgtggaag cggcattgta tttgaaaata
    55441 agatcgttgg tggtgctgtt cccaaggaat acattggacc aatcgagaag ggtgttaagg
    55501 aagctgcaga tggtggtgtc cttgctggat atcctgttgt agatttgaaa gtcaccctta
    55561 ttgatggttc ctatcacgaa gtcgactcaa atgaaatggc gttcaagatg gccgcaatta
    55621 tgggtttcaa ggaaggcgta caaaaaggaa accccatttt gcttgaacca atgatgaagg
    55681 ttgaagttgt cgttccagaa gaatatcttg gtgatgttat ggggcagatc aacagccgcc
    55741 gtggtttgat ccaaggtatg gaagttcgtc ctggaaatgc ccaggctgta aaagccatgg
    55801 ttccgttggc agagatgttt ggttatgcaa cacagcttcg ctctgcaacc cagggacgtg
    55861 gtgtattcaa tatggaattt gaccactatg cgccagtatc gcagtcagtg gcggaagaaa
    55921 ttttaaaagc gtaaccgttt tttcataatt caaggagttt tttatggcga aggaaaaatt
    55981 tgatcgcagc aaaccgcatc taaatgtagg gaccatgg
//
LOCUS       DAOQLC010000009        52503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_151_length_52503_cov_23.570737, whole genome shotgun sequence.
ACCESSION   DAOQLC010000009 DAOQLC010000000
VERSION     DAOQLC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 52503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151_length_52503_cov_23.570737"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1308)
                     /gene="metK"
                     /locus_tag="PL000_02980"
     CDS             complement(94..1308)
                     /gene="metK"
                     /locus_tag="PL000_02980"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNC87886.1"
                     /translation="MSNTFMTSPSLMFTSESVTEGHPDKICDQVSDAVLDACLEQDPR
                     SRVACETAVKTGYVMLLGEITTNAVFNYDELVRKVVNDIGYDSSEKGFDGNSCGVLVA
                     IAKQSGDIAMGVNQALETRDGHKMSDAEVDSIGAGDQGMMFGYACNETPTLMPLPIYL
                     AHKLTRRQSELRKSGAIKWLRPDAKSQVTVEYSKGQPKRIDTVLISTQHSPEVSQNEI
                     TEIITEELIMKTLPENLVDKDIKVFVNPTGRFVVGGPMGDAGVTGRKIIVDTYGGMGR
                     HGGGAFSGKDPTKVDRSAAYAARYVAKNVVAAGLADRVEVQVSYAIGVAHPLSVNVET
                     FGTARIADDKIAALINEHFDLRPGAIIRDLDLRRPIYRKTAAYGHFGRDDIEFPWEKT
                     DKAAALKKAAGL"
     regulatory      complement(1382..1487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1608..2558)
                     /locus_tag="PL000_02985"
     CDS             complement(1608..2558)
                     /locus_tag="PL000_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC87887.1"
                     /translation="MKKTLLSLILLLTACQSAPTPAPAPEETVTVAPSETPAPTVVSF
                     TATPEATFTPVPTATPETIKISTADGMTQLLIPAGTVKMGGLDVLLENDEIPAHDVTL
                     DAFWMDQVEVTNGMYNLCVQAGVCRLPVKLNSDNRGDYFGNPEFQDYPVIQVTWFDAN
                     TYCQWAGRRLPTEAEWERAARGDDLRTYPWGGEPPNELNSNANNIVGDVTRVGSYPAG
                     ASPFGVLDMAGNVWEWVADYYSGRYYGESPAINPLGPEHGGLNFLRVIRGGSFQDNMF
                     NLRVSNRGYETGPDPSAFPGEPEYYGRSSVKIGFRCANAD"
     gene            complement(2610..3854)
                     /locus_tag="PL000_02990"
     CDS             complement(2610..3854)
                     /locus_tag="PL000_02990"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNC87888.1"
                     /translation="MSKRIRNILIGLAGLFLLAVLVYQIPYVNSVLSWRIEKFMIYTK
                     NVFDPVGPVPTALPVTPHPITPTSIATATETPAPVIDLTPSITPTATFAPLPAQASIA
                     SPPFEQQTPNNCGPATLSMALHLYGWKGNQADIANVIKPILQDRNVNPEELRYYVRTQ
                     AGWLNMEYRVGGSIETLKRLIAANYPVIVESVTSLDPNDALGPNDDLWAAHYLLLTGY
                     DDNTQTFTIQDSYHGADLSLPYSQLEKEWKPFNNLYIVIYFPQFEDEMKSLLASNWDA
                     SLNRQLALSASETDTAQNPTDAFAWFNYGSNLVYFDRYEEAAQAYDKAREIGLPLRMF
                     RYQFGPFLAYFHSNRNEDLLVLTDYARGVTEMSEEAWLWYGYGLYRNGDYDGALKAWQ
                     KADQINPNFYEDQAKNAISLIQ"
     gene            4057..4566
                     /locus_tag="PL000_02995"
     CDS             4057..4566
                     /locus_tag="PL000_02995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNC87889.1"
                     /translation="MMKNTTLMTLDPEKLRSAMRAWSAGVTVVTAVHDGHKHGMTVNS
                     FTSISLEPAMITVSLRTGTRTHELVTKSGAFGLTILSAEQADISNLFSGQVTGIEDRF
                     SNLNTETMVTGSPLIAGGLAWLDCRVVQTFDAGMNTLFIAEVLAAQEANDGPPLIYHN
                     RKYWNLSEI"
     gene            4620..5195
                     /gene="amrA"
                     /locus_tag="PL000_03000"
     CDS             4620..5195
                     /gene="amrA"
                     /locus_tag="PL000_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNC87890.1"
                     /translation="MNDQLTDGEKQILLRLAREAMDHAVRGMKLPPLENESLSPRLRE
                     NGASFVTLTIHGELRGCIGALEAYQPLADDVREHAIAAALSDPRFPPVGENELNRIKL
                     EVSRLTVPHLLEYSSGEELPVKLRPHVDGVILKDGFRRATFLPQVWEQIPDAEDFLDH
                     LCSKMGARRDLWRKTKLQVYIYQVEEFHEMN"
     gene            5205..7037
                     /locus_tag="PL000_03005"
     CDS             5205..7037
                     /locus_tag="PL000_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC87891.1"
                     /translation="MAEDRPDDTMFQDAVDALRRGDKARAKELITLLLKADQNNPTYW
                     IWLSAAMDSTKERIYCLQTALKLDPENGTAKRGLILLGALTPDESIQPFPMNRPRAWE
                     EKLLLANEKPKEKGFKAFARSPITRLAFVLLIGAGLVSAVIFGFILPRRSNIRPTETN
                     TPGPSPTFTTTPTLFGATAPPTKAVIGPTPLWMFLPQTYTPTAVYVNTPRAAQSGDQY
                     RLALAAYAQENWDEYITNMQIIASLEPEAADVPYLIGDAYRFKGDADSALDFYNEALK
                     INEDFGPAYLGLARARLLDNPKANVDALFEQAIDLDPDFGEVYLERARYNIINNDPEA
                     AIVDLDRADKLMPNSPVVYMAYADAYLAMDDKKGALEAAETAYSLDITNLEVYKLLGK
                     LHIEGGEYARAIEALDVYAVYETEDDQVFALLGQAYYEMGDYETATSNFEKAEAINKN
                     GLRKFLVYKGLANLELNNLDAAVEDLEKAASADDTSFEINLGLLRAYYLQEKFGSAFL
                     KAEALKSLAETDEETAVMLYWHALVQEKRGEAREAIKDWKALLALDADVMTEQMRADA
                     ELHLKTMVTPTNTPKGGTTKTATPGKTPTPKGGTGTPTPTPTPK"
     gene            complement(7139..7489)
                     /locus_tag="PL000_03010"
     CDS             complement(7139..7489)
                     /locus_tag="PL000_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HNC87892.1"
                     /translation="MSEKPTGVTANKSKKEFTVTWDDNHTSVYPFGLLRAACPCASCR
                     GGHENMKSEPDAEVFTRQMEDSQSTRLANVVAVGSYALTIVWEDGHDYGIYNWHYLRA
                     LCPCAECRGKAKKQ"
     gene            complement(7583..8059)
                     /locus_tag="PL000_03015"
     CDS             complement(7583..8059)
                     /locus_tag="PL000_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87893.1"
                     /translation="MDFKPYFNRWTIFGAIAFAGILLIVTLIIIGWTSPRFTPDVGFA
                     PADLTMIPAPTHTPNAPLVPTLDPSLVTPTLAPGTIGIGAYVQISGTEGEGLRIRSIA
                     GLTGDTVFFGGESEVFLVRDGPQTADGYTWWYLVAPYDENRAGWAAADFLAVVPSP"
     gene            complement(8109..>9071)
                     /locus_tag="PL000_03020"
     CDS             complement(8109..>9071)
                     /locus_tag="PL000_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNC87894.1"
                     /translation="GIILPMRKGLLLYNPAAGRYPVRRYVRGIMHPLKEAGWSIEIAE
                     TLSGTHATQTAHQAAAEKYDAVFAIGGDGTVGQVASGLLDSDTALAVLPAGTTNVWAI
                     EQGQKPFSWFNWWSLRENAKLLANVDVQQVDVGMCNDQPFLLWAGVGLDAHTINKIEP
                     RHRFFKHISVPHFFATTVWEATFWHGLDLRVWTDDKLVEGHYLVAVATNIRHYAGGMS
                     VLSPEALLDDNEMDMWLLSGNSVADALRHFFDLVAGRHLTSDQARRLPFHTARVESDA
                     AFSIQVDGDPALGSSHADITIKHRALKILMPKDALYLLKNPHKH"
     assembly_gap    9072..9171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9195..10316
                     /locus_tag="PL000_03025"
     CDS             9195..10316
                     /locus_tag="PL000_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC87895.1"
                     /translation="MTDETLLNDRYQLLERLGTGGMADVFRARDLMLDRYVAIKVLRK
                     DYSTNEEFQKHFRMEARSAANLSHPNIVTVHDFGFADDLLYIVMEYIPGKDLKQLIRE
                     RGRFSVETGIPLIIQACAGLGYAHRAGLVHCDVKPHNMLVSEDGRLKVTDFGIARALA
                     TMTPGERTDIVWGSPLYFAPEQAQGEAPSPASDVYSIGVVMYELLCGTPPFTASTSDE
                     LARLHISARPIPIREYIPDIPTALEEIIMKVLSKEPAARYRTADQLGRVLLKFGTQPD
                     APAPAPTPVPPPATVISVSPEISDKLPTKETVSTSRQTTAPKQYTRPEPAPQESPEPV
                     SAFEEIDWVFWGLALFALLAVGGLIPFWMMVYFAYNPPI"
     gene            10358..11017
                     /gene="rpe"
                     /locus_tag="PL000_03030"
     CDS             10358..11017
                     /gene="rpe"
                     /locus_tag="PL000_03030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC87896.1"
                     /translation="MNKFLLAPSIIAADFTRIAEEIAACESAGADWLHVDVMDGHFVP
                     TITIGPLFTEACKRVTKLPLDVHLMISEPEKYLEAFAKAGANNITVHVETCPDLVGTV
                     KKIKSLGCKAGITLNPATPASALDSALPLVDLVLVMSVVPGYSGQAFMPEMVGKVEEI
                     RARLDALHSTAHLEVDGGMSAKTLPLMYKAGADMFVAGSAAFGHPQGVGQGIKILREC
                     V"
     gene            complement(11078..11263)
                     /gene="rpmB"
                     /locus_tag="PL000_03035"
     CDS             complement(11078..11263)
                     /gene="rpmB"
                     /locus_tag="PL000_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC87897.1"
                     /translation="MAKCSTCGKATTFGHNRSFSLRATNRTFKPNLQKVSVMENGRLV
                     KKTLCAKCIKTLGKSAA"
     gene            11518..11874
                     /locus_tag="PL000_03040"
     CDS             11518..11874
                     /locus_tag="PL000_03040"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNC87898.1"
                     /translation="MGDETTTLGGIHISPNAVATIAYYATLQSYGVVGLAPKNLADGI
                     VSTITREPSRGITVRYKGEDIDIDINVVVEYGTRINSVAVSVANTVRFHVEKALGLRV
                     NSVNVHVAGLRISDTD"
     gene            11890..13557
                     /locus_tag="PL000_03045"
     CDS             11890..13557
                     /locus_tag="PL000_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNC87899.1"
                     /translation="MGVDSARVEKLRRMPIDGQSLKRLIDAGLTWLRTNKETVNALNV
                     FPVPDGDTGTNMVLTLQAAWNEVKDSGTRNLGEMAAMVSKGALMGARGNSGVITSQIL
                     RGFSRGVHEKATLDVPTLVKAFGEARDTAYKGVVRPVEGTILTVIKEVANATEAALGS
                     AAERDAIEILEVAVKAADEAVKKTPEMLPVLKQAGVVDSGGKGLFFILEGMLRHVYGE
                     SLETPTMSVKPMSAMNLQGALEEVEEGQDYEVVVDFVPTGELDLGAFYGRLEEMGTSI
                     QVGEGEGMYRMHIHVPTEKRYEPIDYIMGIGTVTKVAIENLLAQMDDIQKSKQIQFAT
                     VEPGHIAVVVVSPGAGISRIFASLGVAAVVAGGQSMNPSTQDILSSFENLPTDKIIIL
                     PNNKNIILAANQAKEVTVKQVAVVPTRTVPQGLAAMLSLIPDGELDSVADKMTKALGS
                     VKTGEVTIATRTVEIDGVSVKDGQVIALLDGKLVVSAESVEQGVMELLEKADADQHEL
                     VSLYYGEGMTHAEANRIADLIVTKYTALEVEVQEGGQPHYQFIISIE"
     gene            complement(13659..14678)
                     /locus_tag="PL000_03050"
     CDS             complement(13659..14678)
                     /locus_tag="PL000_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87900.1"
                     /translation="MNLIDRYVTEVGKNLPILKGREDIEKELKSTLEDMLEDRVEQTG
                     QPRSEAMEIELLKEYGSPQKVAATYNPQPYLIGPRLFPFFLMVLKIVIPILVTVLLVL
                     TGIRAATDTPFMGAGFMNIVSKGLGGIISAAITAFGNIVFIFAILERVLPDKEIGDFS
                     NEKDWDPATLAKEPDPDTAKRSDLIAEIVFTFIGLAILNLFPDILGMSYFAEDKMVFI
                     PMFSAEFFKFLPWINAVFLAEIVLDIYLLRSAVWTSLTRIAKIIIEVASIAVAVAILR
                     TPNIFGFTAESFQSVPNSSINADLLLNIFTLSFSIAIIIAIIVAGVDMAKAAFGLLRM
                     NYKRK"
     gene            complement(14688..15017)
                     /locus_tag="PL000_03055"
     CDS             complement(14688..15017)
                     /locus_tag="PL000_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC87901.1"
                     /translation="MANNESLENVTLELRRGVIVLAVLSQLSSEQYGYSLLKLLSDQG
                     LEVDQGTLYPLLRRLETQGLLESVWKLEEARPRRYYIISAEGKKILPKLKKEWAGIVA
                     VMAKVLA"
     gene            15108..15944
                     /locus_tag="PL000_03060"
     CDS             15108..15944
                     /locus_tag="PL000_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC87902.1"
                     /translation="MKIGIVTDSTSDLPAHLIEQHEIHVIPSILVLEGKEYMDGVNIT
                     REEFYTRLPALRTPPTTAAPSIGDFTAPYEALLSKGCDHIISIHPAVKLTTTINVARK
                     AAEEFPGKVTCVDSGSLSLGIGFQILAAAEAAEFGLQSALDAITSTQKRLHVFAALDT
                     MEYLKRSGRVPGAVAALGGLLSIKPMIELKDGEVKPVGAVRITRQADERILNLLLERG
                     ELERLAILHTNAEPRAKNLLDDLMNQARKSVPREILFVNVTAVIGTHLGPNGIGFAAV
                     RK"
     gene            15970..18537
                     /gene="recG"
                     /locus_tag="PL000_03065"
     CDS             15970..18537
                     /gene="recG"
                     /locus_tag="PL000_03065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNC87903.1"
                     /translation="MQPSLEKLRKFFRLEHENKYENTAIIGGLAKMLDYWEGEARADG
                     VAEEVIQAVAARLRSYEKLSPDGRAESLKGLWKRIGETYPEAGQKPKAPPQPSKANQP
                     PRPPRPEPTDPAQLEAPTPPVPPESKPQPRPQAQEQRKPRPTPPPQQQRSRFESAPGA
                     KTSQTPAALNADITVLQGVGPKHAESLKSIGIFTLGDMLYYFPRRYEDYSQLKPIKSL
                     FYGEAVTVLGTVQSVHTRPIRGGKSSIVEVIINDGTGGLRLSFFNQPWLINRMKEGEA
                     ISVSGKVDQYLGRLIMNSPDWEPIEVENLHTARIVPIYALTEKVTQKWLRKLMNEVIP
                     FWAPAVVDILPEKIKLSARLVSLSEALMQVHFPVSQSRLKAARERLGFDEIFFLQMGV
                     LRQKRDWKDAEARRFSVSTEWMDSRLSGLPFTLTSAQQKSLEDIRADLNSGRPMNRLV
                     QGDVGSGKTVVAALGSSVVVSEGAQAAIMAPTSILAEQHFRNFSNLLAGPQGFLQQSE
                     IRLLVGNTTESEKEAIRQGLADGSVKIVIGTHAVIEPDILFKDLQFVVIDEQHRFGVE
                     QRKELRSKGTNPHLLVMTATPIPRSLALTVFGDLDLSVIDEMPVGRQPIDTYVLRPQE
                     RERAFTMIRGQIRSGRQAFIVYPLIDESEKIEARAAVDDFDVLSKEVFPDLKLGLLHG
                     RMRPSEKDEVMLKFRDREYDILVSTTVVEVGVDVPNSTVMLIEGADRFGLSQLHQLRG
                     RVGRGAAQSYCLLIPTHEDATENERLQAMAKTNNGFELADLDLKTRGPGEFLGTRQAG
                     FGTQLKMASITDVSLIEKARTQAQALFERDPYLQNPEHALLAEAFERFWGTGKGDVS"
     gene            18627..19142
                     /gene="coaD"
                     /locus_tag="PL000_03070"
     CDS             18627..19142
                     /gene="coaD"
                     /locus_tag="PL000_03070"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNC87904.1"
                     /translation="MVRALFPGTFDPIHYGHMDIASRASKLFDEVVMAVYDKPLKSLM
                     FSPEERISLVTEAFKDNPKIKVTGYSGLTIDFARKIQAQVIVRGLRVFSDFEFEFRMA
                     LANQRLAKDIETVALITAEQHTFLSSSTVREIAMLDGDVSSMVPAHVKSALHVKVINM
                     GEQSPPNALRD"
     gene            19205..19684
                     /locus_tag="PL000_03075"
     CDS             19205..19684
                     /locus_tag="PL000_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87905.1"
                     /translation="MDILQLIDRLEELFNAAKAVPFTHNVIVDEDRMLELIDQMRIAI
                     PEEVKKAQQIMAQRDRVMAQAQEEANRTLQLARDKADQLTQKDMVVQEAQRRADQIIN
                     QARGEAEATRADADNYVVDTLMQLQDQIAKMSSQVSNGIRMVQEDQMRKSVSSEKVE"
     gene            complement(19760..20437)
                     /locus_tag="PL000_03080"
     CDS             complement(19760..20437)
                     /locus_tag="PL000_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87906.1"
                     /translation="MSANNFFAKSLRFIGIVLMALTGGFTFLGGAGTFCAAVFPQKYE
                     SMVGLIPYQWLYILFMLGTIAIGVWGIWAAIKLIKGTVDAYRMSLYSLITGVLVGGFH
                     IYMSRMLRGKSMPVDAVVYTTLLTLVIFLLFKIPMIWQGVDFSKAKASDNKKAGGAAA
                     FVVGIFTLTIQYTMGPTHTWDGINYADAFNTAMTVIGIGLLVLGTGIFVSLGNVGKTA
                     LRLQVQQ"
     gene            complement(20495..21100)
                     /locus_tag="PL000_03085"
     CDS             complement(20495..21100)
                     /locus_tag="PL000_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87907.1"
                     /translation="MKMSSLDRTLLLFTGFLAAYQIAIGIDQMDSVSITAYTIAFGVI
                     LVAGLLLIILGFEVLDAPIVVIISTIIPLALSLGLIWQHLASLRTVYLIFTIISFLAV
                     VLTRSIAMQNKLPVIVIAITHGIAGLTIFLLPIILAAQGQVKPLFSLVGIGGALIGIG
                     GLLLSFLKTGKPILSRDTIMRLFPMLLLLTTALFVAGFKLG"
     gene            complement(21133..22002)
                     /gene="cyoE"
                     /locus_tag="PL000_03090"
     CDS             complement(21133..22002)
                     /gene="cyoE"
                     /locus_tag="PL000_03090"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC87908.1"
                     /translation="MQAQLSFSTIRSKLGLYIPLIKSLQTSLLLLTGVAGYLSAHTTV
                     NWLHFWQMLPSLFLAISGSTILNMWWDQDIDAKMKRTHKRPTSAGQVTREEVFNLGMA
                     VSVIGIGWAVMVNWLYGLVVFAGLFFDVVVYSMWLKRRTCWSIVWGGISGAMPILAGR
                     VLAVGQIDVIGILLACAVLFWIPTHTLTFSLKFFDDYQSAGVPTFPSTYGEAVTRFAI
                     AFSSVVAALTMGWASVWIGVTAGVLRLIIVLSAGLFFLAITTVFRPSDRVNFSLFKYA
                     SMYMLFAMLLIIP"
     gene            complement(22103..22999)
                     /locus_tag="PL000_03095"
     CDS             complement(22103..22999)
                     /locus_tag="PL000_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC87909.1"
                     /translation="MKRFTLTLCLCAALLASCAPVDLNAPIPTFETGIDPNTWAQIPA
                     GEFHHGQFDETASTEAYEIMVTDVTAAQYADFLNAALADGSIKMDGGKIVGFYPGDTF
                     HGVKHEEKIEAGDWLFIPLDDPSQRIQFDGTTFTVQSGYEKHPMTSVTWFGAWGYCQY
                     FGTRLPTELEWEKAARGTDERPFPWGDEIQRGHANFYSSRDPFEDMASFGSRTSPVGF
                     YNGQVYDGFQTIDSASPYGLYDMAGNVWQWTGDVYEGMHYRFMRGGSKDTYDMDLRIW
                     VRNNATPTYFSPGVGFRCARDE"
     gene            complement(22996..24462)
                     /locus_tag="PL000_03100"
     CDS             complement(22996..24462)
                     /locus_tag="PL000_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87910.1"
                     /translation="MKERFNYLFTSTKGLILVAIAMVGIVTAVWGMLSGPMAEMGVRE
                     IVIKTLGMKIVQAEREGRIIILYHSIAMAIIAIETYMVTGLIKMKPFFKTAINATITV
                     GYILTMVFGMGFAYFGRNWAFHGLYITGLSLIFFAGLMLIVALWPWNPEYHVTDKSYA
                     HTKSGLDLERTAFFATAVTTVISSLFGAVPGSYYGHGFETFLAENIIRLPEKTVMEYS
                     VIGHLHIMLALIGIMITLIIGRWLNFKGAWHKAAMPLMILGTIVLNLGVWGVVTPLEP
                     VAHMVIYVGATPSMLAALFLLIWSWAKLIKDGTAHLTKPTFGQKVGALLRDPLKFGPT
                     WQMLFMNFTTSGLGIFMAIKLDEIFRVWPAREERIELTGHWHVLSAIIATIILLYYGD
                     MNGFKGRLRQWYGWGIIILSDIAFGAVTLFEMKRLFVTEAAQQPLVNGLMYAIDFGLG
                     MLLVILAIIMVHRLIDLFKNKGRWIEELDHELAQEVDK"
     assembly_gap    24474..24573
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24576..25175)
                     /locus_tag="PL000_03105"
     CDS             complement(24576..25175)
                     /locus_tag="PL000_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87911.1"
                     /translation="MKDIVFLLLTGLLAAYLCWYFYQRYQLHKALHNLYYLMGFAVLL
                     VSGLLLIVFGLGILSSPYVLTVASLIPLGVSMGLAEEYFPAWKKYFKWFAALGFLAIA
                     VTSIGGMDLLKKIAVPLFHGVAGLVIFIGPFYAKGAPKGFFWVGIGGLLIGLGGIALA
                     FITLGKQLLFFSPEFVTLILTPLLFLMTGAYVLGFAKRA"
     gene            25412..30259
                     /locus_tag="PL000_03110"
     CDS             25412..30259
                     /locus_tag="PL000_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC87912.1"
                     /translation="MKSLFRPIFFFDPSISRSLQEWRELVLAALLRIVLLIWILSLIA
                     GVNNVFKSYILEVDKTSNPLLQAGIVLSVYLLATFTLVLVTFGKKLPYNLRAWGLLFS
                     VYIVGAIGLVFSSLSGDGRVILFAFIILAAVFFDLRYSVPAFAIGLATMVIVGYLQVN
                     GILVIPPERQLISTDSSSWISGVVVFLTLNIAVLISITNLLRVLGTNLEKSQELLQRE
                     QRLSQILRTVTGINQLIVREQDPHQLLSEACILLVSGQGYSYAWAGLLESDQMTLKLA
                     AQAGDKIDPALFNVRIDELNERGLACAANAIKSRSFFRVDPIKGEDDPCKGCPRRIDN
                     PERVGVALPLLREDRVLGVLVVDHAVPEAVFEDEEIHLLQDLANDLSYALEKIEVNDR
                     LQVYARHQGILNAITQVALETPSLEAMLQKLIENLEGMFGAEGCYFSLWNEETQQVEK
                     FISSDIYKNILGTRPNLNPGEMTFTQSILENRQILVVDDIMNSPFISPRVASLFPVFS
                     AVGLPLIANEKKLGALIFGFRESAPPLSGDDIALAEQTARQIALAMLKVRLDEDTRAK
                     AAELERLYAAAQDMSSSLLDAPELLEKLAHHMTEALSVTSGNIMDINLVTATMQVVGE
                     YWSAEASPREVHSDLGRVFQNEGYGSIIKSMLAGEVLIMHSDDEEMTPTEREQFAEYG
                     ICSMMFVPILAHGQLFGNIEIWESRRRREFSQADIRLAKAMAGHAASIIENAKLVNAV
                     RVSETRYRNLVEQASDGIFLVNPEKMILDANPSGAAMLGYSIEELLAMSVDDIVIAGH
                     SNLLIDELRMGRSMIVEQYVRCKDGSTLPVEISARMLPNGYFQGIVRDISERKQAEKA
                     LAEREAYFRALIENSAEGVAILDEEGVVRYIAPSDEKLTGYSANNVQGTSGFQYIHPQ
                     DLPGVMKIFKEGVATPGAVRTTEYRLRRMDGEWRYFEITGHNMLDDPHIHGVVVNYRD
                     VTERKLAEQALQESRSRLEAIISSALNGIITIDESQHIILFNPSAERIFGYATSEVIG
                     QPLSKLIPDRYHASHGVHVKDYAKSGISARQKGLLDSLFGKRANGEEFPMEAFISNSQ
                     VAGERFFTVIFQDITERRLAEDALRESERKFRALAGNIPSTVYQCKNDERYTVIYLND
                     SVEMLTGYPKEDFLEGGLSFVDLYHPEDAKQIPIPSFVGEDTSSSDLSFHISYRIRHR
                     SGDWRWVDEWGVGVLDDRNAVQYLEGVMIDVTDRKNAEEELRRHAMELETLSVASSAL
                     RTAQNVSEMVPILARHALQAVGGTFASIFLLDSETQDFVSHGWYSVDPGVNFTLADES
                     EVRHKPGTGITGRVALTGEIYITEDIQKDSVITILDGERERLMTVHGGISLPLRTQEK
                     IIGVMHVWSSEPRIFNETDIRLLTALAENTSNAIHRAALFEQTLNHASELATAYDNTL
                     AGWARALELRDELTEGHTRRVTELTIKLAQALNIPEAEIVHIRRGALLHDIGKMGIPD
                     SILHKPGAFTAQERKIMQQHTQYAYDMLAAIPFLQAALDIPYCHHEHWDGAGYPRGLK
                     GTEIPLAARIFSIVDVWDALTSDRPYREAWSKEKTRQYILERSGKQFDPQIVEAFFAF
                     VAQSD"
     gene            complement(30256..31545)
                     /gene="lysA"
                     /locus_tag="PL000_03115"
     CDS             complement(30256..31545)
                     /gene="lysA"
                     /locus_tag="PL000_03115"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNC87913.1"
                     /translation="MMNRIDLFPVSAKIEQNRLTIAGHDLELLAKEYGTPLYLYDQKT
                     MDDAALEYKNALASHYPGPGSVTYAGKAYLCTAIAQWAHGHELYVDCTGEVEIAFAQA
                     GGVPREHILVHGVNKSQADLKSALQHAGTIVVDNLTELKNIAAVSTVASNLWFRLLPG
                     LAVETHHAHTQTGQHGSKFGMTRTEIMEAADICRQHHLPINGIHFHQGSNFRDARPLK
                     EAIGIGLDIAKEIGFPDVWHFSPGGGWGVAYHEDELPQPDTNEYVRVIAENVLAGCQS
                     RGLTLPHLHLEPGRSLVARAGVSVYRAGAIKKRDEKTWVLVDGGMTDNPRHAMYGARY
                     SCLPVSGVGAERSGRISIAGPFCESGDVVIEDLPMRDIHEGDLIAIPASGAYQLSMSS
                     NYNGSRRPAVLWLEKGNARLIQRRETIQDLLARDVMI"
     gene            complement(31535..34087)
                     /locus_tag="PL000_03120"
     CDS             complement(31535..34087)
                     /locus_tag="PL000_03120"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC87914.1"
                     /translation="MLIDLGLFSFYLGVLVLLTLGIAGGAFLNWERPGARTFGFLMLS
                     MMIWAAFYLCEIIAPSLPLKILCRKIMYLGMGISPPLWLGFALRYTGLAPWWSKRGRV
                     FLMTLPGLASFFLGLTNEIHGLIWKSLEIPPGHEIGPLHVIFGTGFWFFTVIAYFFIG
                     MGFLVYIYAFIRSPKTFRIQTGIMLLGALITLIVNLTFVAEPSNNHIDPTPLSFALSA
                     PLLAFGFFRFGLYNLFPIAAPVIMENLHDAIIVTDSQNRITNLNPAAKEWMNAKGQIV
                     GMNIFEAIPNAEMFMEKWDITNEPIKFRLNRSDGVRTWYEASVIPLHKNNAELLGRVL
                     VVHDITQEQELLESEFRRSAHLGLLEEVGRQIASSSDEMEILQHSIHAVVDRFGYGEA
                     AISVLTPDNMMEIRVISGTHDFGYDPGFRQEFGKGIIGHTAKTRKTYVAKNVAEDPYY
                     FSNDEHHGSAICVPILIENKLYGALYVETIESDAVTSEDITTLETLVNQISASLQRVA
                     LYSKAQEHLRVMSAVQAVSRVVISSLDLETIFETVVRELKNSFGYSHVSIYLLKDEYL
                     YLGAQVGYPLEMIIHKIHISQGVSGRTIKTKAVQFIPDASREPDFLRADQTVRSEICV
                     PLLKENVVLGTLNVEGDANDILTREDVELLTTLSSPIALAVDNARLHAQVKSMAMTDA
                     VSGLSNRHALEEYLTAEVERSTRLGHPLSLIIFDIDSFKEYNDKWGHPAGDTRLRAIA
                     DLIRGILRKYDVAARYGGDEFAIILPNTDEHGALEFARRLQNTARAKAAEIHEERKVI
                     PGYTLSIGVATFPKDGNSLAGILLAADHAELTAKRLGKNQIFVASNLYKNDE"
     gene            complement(34096..34473)
                     /locus_tag="PL000_03125"
     CDS             complement(34096..34473)
                     /locus_tag="PL000_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87915.1"
                     /translation="MPNLPISLRRAATFFGIFVLILVVIEFNTRLEELNRLNDQRDQV
                     RAIATQAMQTQVALNTQVAYAESTAAVDEWARTQGHYVQEGDQPVIPIGQPGSEPVIV
                     STPIPTPTPMQNWEVWWSLFFSE"
     gene            34607..35143
                     /gene="rplI"
                     /locus_tag="PL000_03130"
     CDS             34607..35143
                     /gene="rplI"
                     /locus_tag="PL000_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNC87916.1"
                     /translation="MLVKDVYKLGRAGDIKKVADGYGRNFLIPQGFAVLATEGALKQV
                     QKIKAQAEIRRTAQNEELKGLADQIKAVTLTFAAKAGDTGKLYGSITTQDIATALTEK
                     VRFEVKRQQVDIQPIRNLGEYNAHVRLTMDLVPEVKIIVHRDGESAEPAAEEEKADKK
                     KKAKEETAAEEAPAATAE"
     gene            35212..36984
                     /locus_tag="PL000_03135"
     CDS             35212..36984
                     /locus_tag="PL000_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNC87917.1"
                     /translation="MSDSIGQRLKKERENRFLTLERASAETRIRIVFLQALESDDYSV
                     MPSAAQGRGFLRNYAEYLGLNIDEMIAELQKKAAEAAQQQAVDGPLPQVNLSETELPP
                     LTDVKEEKPARPFLSWLGRRPKADSDTVSESTEPAPEETPAPVVEPVEPAAPLEIPVA
                     PVLETPPVEPVEKPKTRGRKKKKTPADEVEAEAVPVQPEPVQEEIAAVQADVTESQLD
                     ALIPSSEENQDEVKEEAKPSLLSRLLGIFSSRPAKSEPELRPVLETRPQQTVPLQPAD
                     VIFAEIGQQLRSRRELISLTYDEVERHTKLRAAFVKALEEGALDRLPSPVQTRGMLAN
                     YATFLDLDVDALLLRFADGLQARRHEKYAETPREKIQMEVVTSMPVLRSFIAGDLIFG
                     VLIISVLVALGIWGVGRVVTSQDEVVAEVTAPSIVQVLGGTPLPTSILNATATIEGTP
                     AETPTVDPLFTPEVPTPVSTANISVSIFAVERTFARVSVDGEVVFEGRFAPGETKLFE
                     AAEQISILTGNGAGLRVTYNGRDLGLLGNIGEVVSRVYLITGVAEPTATISPTPTVTV
                     PATITPVPSSTPSITPTVTPPDGG"
     gene            37060..38415
                     /gene="rimO"
                     /locus_tag="PL000_03140"
     CDS             37060..38415
                     /gene="rimO"
                     /locus_tag="PL000_03140"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNC87918.1"
                     /translation="MSKNTFHLVSLGCAKNTVDSDSMAQLLIRDGYRAMDEPDKAAVL
                     IVNTCGFIGPAKEESYRVLGELAKSKHSGQVLIAAGCLTQRYGVEVAQKVQGIDGILG
                     TRRWMDIVQVVKEARKGKHPEPIYHLPEAATVGVDEQDALRVSVAGASAYLKVADGCR
                     RPCAFCAIPLIKGTAVSRPVDAIVNEARTLRDNGVRELVLIAQDTTDYGHDLGMKDGL
                     AVLLEQLTDSVPDMDWIRVMYAYPGYVTDRLIEVMATRKQILPYLDMPLQHAHPKTLY
                     RMKRPSNIEWVHKTLGKMRAAIPELSIRTTFIVGYPGETDEEYQALYDFVKEIRFDRV
                     GAFQFSFEPGTTSAPLGDPVPASVKQERYEHLMELQQTISLQVNQSYVGKTLDVLVEG
                     FDNGISIGRSYRDAPEIDGMVFIEGKANVGEIVPVRISGAMAYDLTGVLAQSQQQVIK
                     L"
     gene            complement(38502..39008)
                     /locus_tag="PL000_03145"
     CDS             complement(38502..39008)
                     /locus_tag="PL000_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC87919.1"
                     /translation="MTTPASWVMAIIYWLHMLATVTWIGSLAAINLLVLPASQRTLKL
                     ADQLSFIAALQKKLEPLAWFSMGLLVATGLFQLSANSHYDGFFDTSTQWSLSILIKHG
                     LSLIMGIVSAVQTWEVIPAIHRTLMKKEKASPEELASLQRREVILLRTNLILSALILG
                     ATAFARAS"
     gene            complement(39012..40079)
                     /locus_tag="PL000_03150"
     CDS             complement(39012..40079)
                     /locus_tag="PL000_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87920.1"
                     /translation="MSEQTENTQPVTPQPENSQPETVETETAQAESAPQVDDTQPIKP
                     PKKPSRWRSILTGLFGLLLLVGLGAYGGYSSGIGIRQNAEDSVISKQLTEQYAFALTD
                     IQLGNYETAMQRLKFIIANDPGFPGAQQKLTEVMVMSVIPTPTLVPTPTSTPDFSGVE
                     SAYQRAQQLVNAQDWPAALEALDIVRKLDPTYKAAQVDGMYYFTLRNYGIALINQQGN
                     LEGGIYHLTLAERFGPLDNTANQLREGARMYITGASFWELDWKQAYNYFSQVGAGWPG
                     MWDGTMTGSQRLFISAMRYGDELFDQQSYCAAYEQYQIAAGLGTLDNRSQNHFQEAGI
                     QCYPPTEVPPTLEPTPITPAP"
     gene            complement(40362..41867)
                     /gene="murJ"
                     /locus_tag="PL000_03155"
     CDS             complement(40362..41867)
                     /gene="murJ"
                     /locus_tag="PL000_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC87921.1"
                     /translation="MNKLSRLTRNSLIIAFFFLVDKALAFVRTGIISRQYSDSVHMLD
                     TFNAANNLPDVLFALISGGALAMAFIPLLTEYLTTKDRAAAWDLFSRVANVAFLVTGS
                     FAIVIAIFAQPIVDANLGIAPGFGEEQRKLLAELMRLNLIGTIIFSISGLVMASLQAN
                     QHFIFPAMAPSMYNIGQIIGAIFLVPRYGIQGLVYGVIIGALMHLLIQVPMLFKFGFR
                     WTPSLDIRHTGLIEALKLMAPRLLTMGGIQLIVLARDNLASRLDQVGAVTSLTYGWMI
                     MQVPETILGTAIATAMLPALSELAARHDWVEFRSTIERALRVLIALTIPIAAVMAAGI
                     HPLVRGVFGFDEATSTLVTWTTRAYLMTLTGFTIHEIAARSFYARKEPLFPLYAVGLR
                     LVMFLGIGFIGLTFFKEIGAPIIAFAEIALLVEAIILFKWLSNRTHEPIHVGGSVVRG
                     LIAAVIGGVVTYAVALYLPGGAIITALIGMVVGGLIALAIVWSDAKILLKL"
     gene            complement(41870..42061)
                     /locus_tag="PL000_03160"
     CDS             complement(41870..42061)
                     /locus_tag="PL000_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87922.1"
                     /translation="MTEQNEKNCLNCNRTDEQVPLLHLTFKNETKYICPQCLPVLIHK
                     IHLIADKLPGVEIAPDAAH"
     gene            complement(42129..42917)
                     /locus_tag="PL000_03165"
     CDS             complement(42129..42917)
                     /locus_tag="PL000_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC87923.1"
                     /translation="MDLQLKNKIALVAASSRGLGYAAARTLAQEGCRVAINGRDEAKI
                     KGVAEKLSKETGTEVIGLGGDVSLPHVPEMLVAQTVEAFGGLDILITNTGGPTAGSID
                     ALDEAAWQKGIDLCLMAHVRLIKSALPHLRKSKSASVLTVTSYSVRQPIPNLLISNSV
                     RAATVGLTKSLALELGRENIRVNSILPGWTETERVTELMTDRAKKNSSTVGEETERQA
                     AESPFGRMGQPEEFANAAVFLVSPAASFITGVMLLVDGGTVKGV"
     gene            complement(42972..>43270)
                     /locus_tag="PL000_03170"
     CDS             complement(42972..>43270)
                     /locus_tag="PL000_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNC87924.1"
                     /translation="IENQTMLAILTIHLRLPLCTSLKEKRGRIKPLLSRLHREFNVSV
                     AEMDLQDTWDEAIIACAMLNSDAVVLRQSLQSVAKWVEANWTDGDVWDSKIENL"
     assembly_gap    43271..43370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(43448..44014)
                     /locus_tag="PL000_03175"
     CDS             complement(43448..44014)
                     /locus_tag="PL000_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87925.1"
                     /translation="MKNWENFIKSNKTFIYIGIAFVCIGLGGLFSVKFLHQNLVIGIF
                     IGAMAATLILVAIAPSIEHKFRKDSLGQQIKRPSSPLFKDLRAYLHPTRRHTTIGFLW
                     CFFNIFFIVPLFGKQSENLSTVNSISILFLLFISLDGYQMVKRNVRISSKYGYLIHGA
                     GSQYIGYLILFFCWTGCIVLILSMIIGW"
     gene            44055..45377
                     /locus_tag="PL000_03180"
     CDS             44055..45377
                     /locus_tag="PL000_03180"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNC87926.1"
                     /translation="MDIETKYNQALDYLYSFVDYSLKHSSELAKADFNLDRMFALMES
                     LGNPQKKYPIIHVAGTKGKGSTSALCASALRAAGYKVGLYTSPHLLDYTERIQVNGAP
                     ISHEQLVALVDEIRPHVSKIEKLTTFEITTALAFMAFAKYGMDAAVFEVGLGGRLDAT
                     NIVTPNVSIITSLSYDHMAVLGNTLTLIAGEKAGIIKPGIPVVSSPQKDEAFQVLERV
                     AKEKSAGLTLVGRDVTFELVESSLDGQKFNLQPSTFNHHPLSFVIPLLGAHQIENAAT
                     AYAALKISGIPITDEQIQKGFSQVQWRARFEVARREPPVIFDSAHNQDSFQKLGETLE
                     TYFPGKKVILIFGASEDKSILDMFTALKPKIQKIIVTRADHPRALEVERIQQLAGQAG
                     AESEAVTPVKSALMRALELSSNDGSIVLSAGSMFVTAEVMSAWKTRNE"
     gene            45374..47848
                     /gene="lon"
                     /locus_tag="PL000_03185"
     CDS             45374..47848
                     /gene="lon"
                     /locus_tag="PL000_03185"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC87927.1"
                     /translation="MNYQSDWNENEDNFDLTLSQRTEELYRVPNMEPNADGLIEAVVL
                     PLRDMVIFPRMVSPIFVGREASLLAVREAQAKEQTVIGLTQKDPDLNDPNAEDFLPVG
                     VEMAVGRMLQMPDGSYSALVQGRKRVEIVEFLRVKPYIKVRARVINEPTSADRQTQAL
                     MRSVLSLFERCVQLDRSIPEEAHLFAMNIAEPGWLCDMIATSLSLTYEAKLRLLLVLD
                     PRARLGLLNNLLAQEVDVLELEDEIHSRVQSEVDKSQREFYLREQMKQIQNELGEGDV
                     FSRDASDLKKKLDAANLPDEVRKVAMKELERLSQMPPMAPEVGMIRTYIDWIVDLPWS
                     NSSPDNLDVKNAAKILERDHYGLKKAKDRILEYIAVRSLKPKKERQPILCFVGPPGVG
                     KTSLGRSIADALGRKFVRVSLGGVRDEAEIRGHRRTYIGALPGRIIQTMKRAGTTNPL
                     FMLDEIDKLYSDFRGDPASAMLEVLDPEQNYAFSDHYLELPFDLSKVMFVTTANSLGS
                     IPSPLLDRMEIIEFPGYIEEEKVEIANRYLIPRQLEESGVDNLGLVFEPAALQRIIRE
                     YTYEAGVRSLEREIGRVCRKVARMKAEGKKYPLAIAPELIEKLLGPQQVFPPEAERSD
                     EVGVATSLAWTETGGEIMSVEVAVLEGKGGMQMTGQMGEVMQESAQAALTYIKSRASK
                     LKIELEAFERLDIHVHMPEGGIPKDGPSAGITMATAIISALTGRPIFHHVGMTGEITL
                     RGRVLPIGGVREKVLAAHRAGLKTVLLPEKNMKDLVDLPKTAKSELKIIPIKHMDDVL
                     ALALSEKVTVEPPRPKKQARDDSQED"
     gene            47991..48800
                     /locus_tag="PL000_03190"
     CDS             47991..48800
                     /locus_tag="PL000_03190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC87928.1"
                     /translation="MSKLQNKVVLITGASSGFGEDAAMLFAQEGCKVVLAARRMDRLQ
                     NLAERIQSAGGEAIAIPVDIVNVADVDNMVQTTLDLYGHIDILFNNAGIGRVEWFENH
                     TLERDLDTLVHVNLTSLMQVTRSVLPHMLARGEGHIINMISVAGLLATPLITSYAASK
                     HGVRGFTDALRREVAPLGIKVSGIYPGPASTEFGQHVGKNEAYRSVRSRLKIHMSSSY
                     VARRVVDVAKRPRRSLIIPWWFRILTTIEAVFPALVDWISYSFTKKKHKLD"
     gene            48810..49916
                     /locus_tag="PL000_03195"
     CDS             48810..49916
                     /locus_tag="PL000_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNC87929.1"
                     /translation="MTTARLKSLIDSLPTSNLDAVILNPGPTLTHLTGVHFHLMERPV
                     VLFVAPGQDPALVLPELELPKLGLFPYKVQAFAYGENPSEWDDAFRKAAQALGLDGKR
                     IGVEPRQLRLLDFRHVKAGAPEAEYPDASEVLSRLRLKKDKAEVDAMRRAVKIAQDAL
                     EATIPLIKIGMTERELSSELVIQLFKQGSDPELPFAPIVSGGPNAANPHASPTERKLQ
                     AGDLLVVDWGAAFDGYISDLTRTFAVGEVDDECNKIHKIVQEANAAGRAAGGPGVSCA
                     NVDKATRDVIEASGYGKYFTHRTGHGIGMEGHEEPYMRGDNMQLLEPGMAYTVEPGIY
                     LTDRNGVRIEDNVVITENGAESLSDMPREIRVVG"
     gene            49923..50396
                     /locus_tag="PL000_03200"
     CDS             49923..50396
                     /locus_tag="PL000_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87930.1"
                     /translation="MQDPVYVICRATSTDADRLTEITIAAKRHWNYPEKWIQFWLPQL
                     TLTAEYISKHEVWMVMAKDAAIGYYSLEYSDGELWLGNLWVLPEHMGQGIGRLLFEHA
                     LNRCRGLGCSTLKIEADPNAQSFYERMGACKIDEHCSEVDNQPRILPVMEIVVPA"
     gene            50525..51298
                     /locus_tag="PL000_03205"
     CDS             50525..51298
                     /locus_tag="PL000_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNC87931.1"
                     /translation="MSEFTPTPERQKQILSLLGKQGRLSVAEIVEQFSISEATARRDL
                     ETLASQGKAQRVHGGVIALEQAPPELPILEREGEQTDEKASIGRAAAELIADGETVFL
                     GSGTTVLEAAKHLRDRKNLTVITNSLPVLNVLAGIKDITVVSLGGQLRESELSFIGHI
                     TEQALAELRVDKVIMGTRGISLEHGLTNDYLPETLTDRAILKIGRTVIIAADHTKINR
                     VATALLAPLKSMQVFVTDSHAEKKFLQSLKKLEIEIVIA"
     gene            51324..52358
                     /locus_tag="PL000_03210"
     CDS             51324..52358
                     /locus_tag="PL000_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC87932.1"
                     /translation="MLLKNFRPQSKLVTKTTLVNKPKFPVIDAHDHLGEPFGGGWDKK
                     PLSELLDLLDEAGVTHYVDLDGGWGEDILDAHLKHFKEGAPERFQVFGGVDWNKWQEL
                     GNQFPEWAAKRLIAQKERGAQGLKIWKPLGLHVKDHQGKLVDVDDIRLSPIWETAGEL
                     GFPVLIHVADPVAFFDPIDETNERWEEIGEHPDWAFTSPPFPSFMHIMNCFKNLVTRH
                     SSTTFIGAHVGCYGENLGWVGQMLDDCPNYYIDISARFGEIGRQPYTSRRFFIQYQDR
                     ILYGSDMSPDLDAYRLSYRLLETDDEYFNYNTSEVPGQGRFYAYGLYLPDDVLKKVYR
                     ENAMKVLKFT"
BASE COUNT        12744 a        13273 c        13838 g        12348 t
ORIGIN      
        1 ttgccattac cataatccac ttcgttatgt aaaaaaagag ccgcggactc aagtctcgcg
       61 gctcttttca attaccaatt accaagcact aacttacaac cctgctgcct tcttcaaagc
      121 cgcagctttg tcagtctttt cccacgggaa ttcgatgtca tcgcggccaa agtgaccgta
      181 agccgcagtc ttgcgataga tcggacggcg caggtcgagg tcacggatga tcgcgcctgg
      241 gcgcagatca aaatgctcgt tgatcaaagc ggcgatcttg tcatctgcga tcctggcagt
      301 gccgaaggtt tccacattca ccgaaagcgg atgtgccaca ccgatggcat acgaaacctg
      361 cacttccacg cggtcagcca gccccgctgc aactacattc ttggccacat agcgcgccgc
      421 atacgctgcg gaacgatcca ccttcgtggg gtctttcccg gagaacgcgc cgccgccatg
      481 acgacccatg ccgccgtaag tatccacaat gatcttgcgg ccagtcacgc ctgcatcgcc
      541 catcgggccg cccaccacaa aacgacctgt cgggttcaca aagaccttga tatctttgtc
      601 cacaaggttt tcgggcagag tcttcatgat caactcttca gtgatgatct cggtgatttc
      661 attctgcgaa acctcaggcg agtgctgggt ggagatcaac accgtgtcaa tgcgcttcgg
      721 ctgacctttc gaatattcca cagtcacctg gctcttggca tcggggcgca gccacttgat
      781 cgcgccgctc ttgcgaagct cgctctggcg gcgggtgagt ttatgcgcca gataaatggg
      841 aagcggcatc agggtcggtg tttcgttaca ggcataccca aacatcatgc cctgatcgcc
      901 cgcgccgatc gaatccactt cggcatcgct catcttgtga ccatcacggg tctcaagcgc
      961 ctgattcaca cccatggcaa tatcgcctga ttgttttgcg atcgcaacca gcacgccgca
     1021 ggaatttcca tcgaaaccct tctcgctcga gtcgtatccg atatcattca ccaccttgcg
     1081 cacgagttca tcgtaattga aaaccgcatt ggtggtgatc tcgcccaaca gcattacata
     1141 acccgtcttc actgcggtct cgcaagccac acgtgaacgc ggatcctgtt cgaggcacgc
     1201 atccaacacc gcatccgaaa cctgatcgca gatcttatcg gggtgtcctt ccgtcaccga
     1261 ctctgatgta aacatcaggc tgggcgaagt cataaaggta ttgctcatcg tttttgtttc
     1321 tccttgaaaa atataattgc cctttcgacg tgaaagggca atcgtataaa acttatctac
     1381 cctctcatct cccagaaatt tctgtcggat ttggcaccat attcaacttt cgctgactgg
     1441 ttgtcgaggc atcgcagggc cgttccctcc gcctctctgg ataagagcgc cgtattgggg
     1501 ctgttgaatt gtttcgcgat aatatcatag ataacttgca gcgtaaagat aaaaatatta
     1561 acgaagatgg agaaccgctt cgctcttaac tgtagtttga ttctgcttta atcagcattc
     1621 gcacaccgaa acccgatctt caccgaactg cgcccgtaat attctggctc ccccggaaaa
     1681 gcggatgggt ctggtcctgt ctcatatcca cgattggaaa cgcgcagatt gaacatgtta
     1741 tcctgaaatg acccgccgcg aatcacacgt agaaaattca acccgccatg ctcgggcccc
     1801 agtgggttta tggctggtga ctctccgtaa tatcttccac tgtaataatc cgccacccat
     1861 tcccacacat tgcccgccat atccagcacg ccaaacggac ttgcccccgc aggatacgaa
     1921 cccacacggg tcacatcgcc gacgatgtta tttgcattcg agttcagttc attcggaggt
     1981 tcaccgcccc agggataagt ccgcaggtca tcgccgcgtg cggcacgttc ccattctgct
     2041 tcggtgggca gcctgcgccc cgcccattga cagtaggtat ttgcgtcaaa ccacgtcacc
     2101 tgaattacag gataatcctg aaactcagga tttccaaaat aatctccgcg attatctgaa
     2161 ttgagtttga cgggcagcct gcaaacgccc gcctgcacac acaggttgta catgccgttg
     2221 gttacttcca cttgatccat ccaaaatgca tcaagcgtta catcgtgtgc agggatctca
     2281 tcattttcaa gcagtacatc cagcccgccc attttcaccg tcccagcagg gatgaggagt
     2341 tgggtcatgc catcggcggt tgaaatcttg atagtttcgg gcgtggcagt tggaacagga
     2401 gtgaaagtgg cctcaggagt tgctgtgaat gaaaccactg tcggcgcagg cgtttctgat
     2461 ggcgcaaccg tgacggtttc ctcaggcgcg ggcgcaggtg tgggggcgct ttgacaggcg
     2521 gtgagcaaaa gaataagaga caggagggtt ttcttcatcg gtggttttct gtgattcatg
     2581 tgcgcgaaat gctaatttgc tttttggatt tattgaatta ggctgatggc gttcttggct
     2641 tgatcttcgt agaagttggg gttgatctgg tctgcttttt gccaggcctt gagcgcgccg
     2701 tcataatcgc cgttgcgata cagaccgtat ccataccaca accaggcttc ttcagacatt
     2761 tcagtgacac cgcgcgcata atcggtcagc accagcaggt cttcattgcg gttggaatgg
     2821 aaataggcaa ggaaggggcc gaactgatag cggaacatcc gcaagggcag cccgatctcg
     2881 cgggctttgt cgtaggcttg cgcggcttct tcatagcgat caaagtagac aaggttgctg
     2941 ccatagttga accatgcaaa tgcatcggtg gggttttggg cggtatcggt ttcagatgct
     3001 gataatgcca attgccgatt caggcttgcg tcccagttgg aagcgagcag gctcttcatc
     3061 tcatcttcaa attgtggaaa gtagatcacg atgtacaggt tgttgaacgg cttccattcc
     3121 ttttcgagtt gagaataggg cagtgaaaga tctgcgccgt gataggaatc ctgaatggtg
     3181 aaggtttggg tgttgtcgtc atagcctgtg agcagaaggt agtgggcagc ccacaagtca
     3241 tcgtttggac ccagcgcatc gttcgggtcg agcgaggtga cactttcgac aatgacggga
     3301 taatttgcgg cgatcagtcg cttaagggtt tcgatgctgc cgcccacacg atattccatg
     3361 ttgagccagc ccgcttgtgt gcggacgtaa tagcgaagct cttcggggtt gacattgcgg
     3421 tcttgtaaaa tgggtttgat gacgttggca atatcggctt gattgccttt ccagccatag
     3481 agatgtaacg ccattgagag agtggcgggg ccgcagttgt ttggggtttg ttgctcaaaa
     3541 ggcggtgacg caatggatgc ctgtgcgggc aggggcgcaa aagttgcggt gggtgtgatg
     3601 gaaggcgtca ggtcaatgac tggggctggc gtttcagtgg ctgtggcaat ggaggttggc
     3661 gtaatggggt ggggggtgac tggcaatgca gttggcactg ggccgacggg gtcaaatacg
     3721 ttctttgtat agatcatgaa tttttcgatg cgccacgaaa gaaccgaatt gacatacgga
     3781 atttggtaca ccaaaacagc caacaagaac aaccctgcca gcccgatgag gatatttcga
     3841 atacgtttgg acatggggcg agtctaccac gcgtttatga atgggaattt aacttgagag
     3901 gagcgcagta ttcggattga aactcatcaa atcatctgcg aaagatggga attttctaaa
     3961 gatgttcctg ttggtaagaa aacaaaaaac gaccttgtcc taaaatcgca tttttgactt
     4021 gacgctctcc catctcacaa ttatgatgaa tttcacatga tgaaaaatac aacacttatg
     4081 actcttgatc ctgaaaagtt acgctcggcc atgcgggcct ggtcggcggg ggtgactgtg
     4141 gtgaccgccg tacatgatgg ccacaaacat ggtatgaccg taaactcatt tacgtccatt
     4201 tcgctggagc ctgccatgat caccgtgtct ctgcggacgg gtacccgtac acatgaattg
     4261 gtgaccaaat ctggtgcctt tggcctgacg atcctttctg cagaacaggc cgatatttca
     4321 aacctgtttt cagggcaggt gacagggatc gaagatcgat tttccaattt gaatacggaa
     4381 accatggtca caggttcacc cttgatcgcg ggtggtctgg cctggctgga ttgccgcgtg
     4441 gtgcagactt tcgatgcagg catgaatacg cttttcattg ctgaagtact tgctgcacag
     4501 gaagcaaacg atggcccgcc cttgatctat cacaaccgaa aatattggaa tttgtctgaa
     4561 atataaattg aggcccgaaa ggtttgataa cctttcgggc ctgtttatgg gaggctacca
     4621 tgaacgacca attgaccgat ggggaaaagc aaattttgct gcgccttgca cgtgaagcaa
     4681 tggaccatgc tgtgcgggga atgaaacttc cgcctttgga aaacgaatct ctctctccgc
     4741 gtttgcgtga aaatggtgca tcgttcgtca cacttaccat tcatggtgaa ttgcgcggct
     4801 gcattggcgc tctcgaggct tatcaaccgt tggcagatga tgtgcgtgaa catgccattg
     4861 ctgcggcttt gagtgatccg cgttttccac ccgttggaga aaatgaatta aacagaatca
     4921 agctcgaggt gtcgcggttg accgttcctc atttgctgga atattcttcg ggtgaagaac
     4981 tgcctgtaaa actgcgcccg catgtggatg gcgtcatctt gaaagatggg tttcgccgcg
     5041 ctacttttct tccgcaggta tgggagcaaa tccctgatgc agaagatttc cttgaccatt
     5101 tatgttccaa gatgggggcg aggcgcgatc tgtggcgaaa gacaaaattg caagtttata
     5161 tatatcaggt cgaagaattc catgaaatga attagaatcc agtcatggct gaagaccgac
     5221 ccgatgacac catgtttcag gatgctgtgg atgctttgcg ccgcggagac aaagcccgcg
     5281 ccaaagagtt gatcacgctt cttttgaaag cggatcagaa taacccaacc tattggatct
     5341 ggctcagcgc cgcgatggat tccaccaaag agcgcattta ctgcctgcaa acagcgctca
     5401 aactggaccc tgaaaatggt acagcgaaac gcggcttgat cctgctcgga gcattgaccc
     5461 ctgacgaaag cattcagccg ttccccatga accgtccacg ggcatgggag gaaaaacttt
     5521 tacttgcgaa tgaaaagcca aaggaaaagg gcttcaaggc ttttgcgagg agccccatta
     5581 cgcgtttggc atttgtattg ttgatcggtg cggggttggt ttctgcggtt atttttgggt
     5641 ttattctgcc gcgccgctca aatatacgtc ccactgaaac gaatacacct ggtccttctc
     5701 caacttttac caccacaccg accctgtttg gagcaacagc gcccccaacc aaggctgtga
     5761 tcggtcccac ccccttgtgg atgttcctgc cccaaaccta tacgccgacg gccgtttatg
     5821 tcaatacgcc gcgtgctgca cagtcaggcg atcaatatcg actggcactt gccgcatatg
     5881 ctcaggaaaa ttgggatgaa tacatcacca acatgcaaat cattgcttcg ttggaacctg
     5941 aagccgccga tgtgccatat ttgatcggag acgcctatcg tttcaaggga gatgccgata
     6001 gcgccctgga tttctacaac gaggccttga agatcaacga agattttggc cctgcctatt
     6061 tggggttggc gcgtgccaga ttgttggata atcccaaagc caatgtggat gccctttttg
     6121 agcaggccat tgacttggac cctgattttg gtgaagtgta tctggagcgg gcgcgataca
     6181 acatcatcaa caacgacccc gaagctgcca ttgttgatct cgaccgcgcg gataagctga
     6241 tgcccaattc gcccgtcgtt tacatggcct atgccgatgc ctatcttgcg atggatgata
     6301 agaagggtgc attggaggcc gctgaaactg cctactcgtt ggatatcacc aacctggaag
     6361 tgtacaagct gttagggaaa ctgcacattg aaggcggcga gtatgcacgc gccatcgaag
     6421 cgttggatgt ctatgctgtt tatgaaacgg aagatgatca ggtctttgcc ctgcttggac
     6481 aggcctatta tgaaatgggc gattacgaga cggctacatc caattttgaa aaagccgagg
     6541 cgattaacaa gaatggcttg cggaagttcc tggtctataa agggctcgcc aaccttgaac
     6601 tgaacaacct ggatgccgcc gtggaagacc ttgaaaaagc cgccagtgca gacgatactt
     6661 cctttgaaat caatcttggt ttgttgagag cctattattt gcaggagaaa ttcggcagcg
     6721 ccttcctcaa ggcggaagca ctcaagtcgc tggctgagac ggatgaagaa accgcggtga
     6781 tgctgtactg gcatgcactg gttcaggaga aacgcggtga agcgagagag gccatcaaag
     6841 attggaaggc gcttctggca ttggatgccg atgtaatgac cgagcaaatg cgtgccgacg
     6901 cagaattgca tttgaaaacc atggtcacgc cgacgaatac gccgaaaggc ggcacaacca
     6961 agaccgccac accaggaaag acacccacac caaaaggtgg aacaggtacg ccaacaccaa
     7021 caccgacgcc aaagtaggta ggtggaaagt gggggagtgt acgggaaaag atgaaagatg
     7081 aaagagaggc attcttttgg aatgcctctc tttattatcc tgattacttt ttcactgatt
     7141 actgcttttt cgctttcccc cgacattccg cacatggaca caatgctcgc aaataatgcc
     7201 agttgtaaat tccataatca tggccgtctt cccagacaat ggtcaacgcg taggagccga
     7261 ctgcgacgac attggccagc cgcgttgatt gagagtcttc catctggcgg gtgaatactt
     7321 cagcatcagg ctctgatttc atattttcgt gtccgccacg gcaggaggcg catggacagg
     7381 ccgcgcgcaa caacccaaag ggatatacac tggtatggtt atcatcccag gtgacggtaa
     7441 attctttttt gcttttgttg gcggttacgc ctgttggttt ttcagacatt tttcctcatt
     7501 ataaaaactt tttgaaccgt gaagcgtgct gtaaacgcga aaaaaatatt tcgggtcttt
     7561 tggcatgctt tgcggtaatt aactacggtg aagggactac cgccagaaaa tctgcagcgg
     7621 cccagcctgc acggttttcg tcatagggtg ccacgagata ccaccacgta tatccatctg
     7681 cggtttgcgg gccatcacgg acgagaaata cttctgattc gccgccgaag aacactgtat
     7741 cgcctgtcaa ccccgcaatg gagcggatgc gcaagccttc gccttcggtt ccgctgatct
     7801 gtacataggc gccaatacca attgtgccgg gagccagggt tggggtgaca aggctggggt
     7861 cgagtgtggg cacaagggga gcgttggggg tatgtgtcgg ggcggggatc attgtcagat
     7921 cggcaggagc aaacccaacg tcaggggtga agcgcggaga ggtccagccg atgatgatca
     7981 gggtgacgat cagtaaaatc cctgcaaagg cgattgcgcc aaaaattgtc cagcgattaa
     8041 agtagggttt gaaatccatg tcgtgtcatt gcgagggcgt tctttctcct gcccgaagca
     8101 atctccagtt aatgtttatg tgggtttttc aataaataca gtgcgtcttt tggcatgaga
     8161 attttgagcg cacggtgttt gatggtgata tctgcgtgag aactgccgag cgcgggatcg
     8221 ccatccactt gaatggagaa ggccgcgtct gattctaccc gcgccgtgtg aaatggcaag
     8281 cgccgcgcct gatcagaggt gaggtgacga cctgcaacga gatcaaaaaa atgacgcagc
     8341 gcatccgcaa cactgttgcc gctaagcagc cacatatcca tttcgttgtc gtcgagcagg
     8401 gcttcaggtg aaagcaccga catgcctccc gcgtaatggc ggatgttggt ggcaaccgcc
     8461 acgagataat gaccttcaac cagtttgtcg tctgtccata cgcgcagatc gagaccatgc
     8521 caaaaggtgg cttcccaaac ggtggtggca aaaaaatgcg ggacggagat gtgcttgaaa
     8581 aaacgatggc gcggttcgat cttgttgatg gtgtgcgcat ccagaccaac gcctgcccac
     8641 agcaaaaaag gttggtcgtt gcacatgccc acatccactt gctgcacatc aacattggcg
     8701 agcaattttg cattctcgcg cagtgaccac caattgaacc agctgaacgg tttttgtccc
     8761 tgctcaatgg cccagacgtt ggttgtgcct gcgggcaaaa cggcgagcgc agtatccgaa
     8821 tccaacaatc cactggcgac ctgtcccact gtcccatcac caccgatggc gaagaccgcg
     8881 tcatattttt ctgcggcggc ttggtgcgca gtttgggtgg catgggtgcc gctgagggtt
     8941 tcggcgattt cgatgctcca gcccgcttcc ttgagcggat gcatgatccc acgcacataa
     9001 cgtctcacag ggtaacgtcc cgcggctgga ttgtagagga gaagtccctt gcgcatgggg
     9061 aggattatac cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtcttgcga
     9181 tataatcccc atcgatgact gacgagaccc ttctcaacga tcgttatcaa ctgctcgaaa
     9241 ggcttggtac cgggggcatg gccgatgtct ttcgggcgcg cgatctcatg ctggatcgct
     9301 atgtggcgat taaagtcctg cgcaaggatt attcgaccaa cgaggaattc cagaagcatt
     9361 tccgcatgga agctcgttcg gcggcgaatc tttcgcaccc caacatcgtc accgtgcatg
     9421 actttggctt tgcagatgac ctgctctaca tcgtcatgga gtacatcccc ggcaaagacc
     9481 tcaaacaact catccgcgaa cgtggccgct tttcagtaga gacgggcatt ccgctcatta
     9541 ttcaggcttg tgcggggctg gggtatgccc accgtgccgg gcttgtccat tgtgatgtca
     9601 aaccgcacaa catgctcgtt tcagaagatg gccgcctcaa agtcaccgac ttcggcattg
     9661 cgcgcgccct cgccactatg acacccggcg aacgcactga cattgtctgg gggtcaccgc
     9721 tttattttgc gcccgagcag gcccaagggg aggccccttc gcctgcgtcc gatgtctatt
     9781 ccattggtgt ggtgatgtat gaattgctct gcggtacgcc accgttcacc gcatccacct
     9841 ccgatgaatt ggctcgcctg catatttccg cccgcccgat tcccatccgc gaatatatcc
     9901 ctgatatccc gaccgcactc gaagaaatta ttatgaaggt gctctccaaa gagcccgccg
     9961 cacgctatcg caccgcagac cagcttgggc gcgtgctgct caaatttggc acccagcccg
    10021 atgcccctgc ccctgcgcca acacctgtgc cgccccccgc aacagtcatc agcgtctcgc
    10081 cagaaatttc ggataaactc ccgaccaagg aaacggtttc cacttcccga cagaccactg
    10141 cccccaagca atatacccgg cccgaaccag cgcctcagga atcacccgag cctgtctctg
    10201 ccttcgaaga aattgactgg gtcttttggg gcctcgcctt gtttgctttg ctggcagtgg
    10261 gtggcctcat cccattttgg atgatggttt actttgccta taatcctccc atttaataaa
    10321 atcaattgtt tacggcgtcg gttttacttt ctgactcatg aataaattcc tcctcgcccc
    10381 ctctatcatc gccgcagatt tcacccgcat tgccgaagag atcgccgcct gtgaatctgc
    10441 tggcgccgat tggctgcatg tggatgtgat ggatgggcat tttgtcccca ccattaccat
    10501 aggtccgctt tttactgaag cctgtaaacg tgttaccaaa ctcccattgg atgtccattt
    10561 gatgatctca gagcctgaga aatatcttga ggcctttgcc aaagcaggcg caaacaatat
    10621 caccgtccat gtggaaacct gccccgatct ggttggcacc gtgaaaaaga tcaaatccct
    10681 gggctgcaag gcagggatca cactcaaccc cgctacccct gcctctgcat tagattccgc
    10741 cctgccgctt gttgaccttg ttttggtgat gagtgtggtt cccggatatt caggtcaggc
    10801 gttcatgcct gaaatggtcg gcaaagtgga agagatccgc gcccggttgg atgcattgca
    10861 ttcaaccgcc cacctggagg tggacggggg catgtctgca aagactttgc cgctcatgta
    10921 caaagccggt gcagatatgt tcgtggcagg cagcgctgcg tttggtcatc cgcaaggtgt
    10981 ggggcagggc atcaaaatat tgcgggaatg tgtttagtcc gcgttaaaac aaaaatcacg
    11041 gccgaagccg tgacctttga tgtgaatgga aaacaaacta agccgcagat ttgccgagtg
    11101 tcttgatgca cttggcgcac agcgtcttct tgaccagacg gccattctcc atcaccgaaa
    11161 ccttctggag gttgggcttg aacgtgcggt ttgttgcacg cagggagaac gaacgattgt
    11221 ggccgaaggt ggttgcttta ccgcaggtgc tgcattttgc cattttagga tacttccttt
    11281 caattacaat gattccacta cctgacctgg caacatgccg ctgggtcagc gcctttactc
    11341 aaaggcgcgg aattttacca tacaacgcaa ccttcttcaa gataagatgt taaaatagac
    11401 acggaaagcc agcgtacggg cggcaagacc gtccgatcca acagaagagc cttgggcatc
    11461 agcagtgtta tgtgctgtaa aaactccctc ggcatgatga ttaaaattga ggaacctatg
    11521 ggcgacgaaa caacaacctt gggcgggatt catatttcgc caaacgcggt ggcaaccatt
    11581 gcttattatg ccaccctgca atcctatgga gtggtggggc ttgcgccgaa aaatctggca
    11641 gatggcatcg tttccaccat tacccgcgaa ccttcacgcg gaatcaccgt ccgttacaag
    11701 ggcgaagaca tcgacatcga tattaacgtc gttgtagagt atggcactcg catcaactca
    11761 gtggctgtca gtgtggccaa caccgtgcgc ttccatgtgg aaaaagcatt gggactgcgt
    11821 gtaaattccg tgaatgtgca tgtcgccggg ctgcgcatca gcgatacaga ttaggagccg
    11881 agaaaactta tgggtgtgga ctctgcacgc gtagagaaac ttcgtcgaat gccgattgat
    11941 gggcagtcat taaaacgctt gatcgatgcc gggctgacct ggcttcgcac caacaaggaa
    12001 acggtcaatg ctttaaacgt tttcccagtg cctgacggtg atacaggcac caacatggtg
    12061 ctgactttgc aagccgcatg gaacgaggtc aaggattcgg ggactcgcaa cctcggcgag
    12121 atggcggcca tggtctccaa gggcgccttg atgggcgcgc gcgggaattc gggcgttatc
    12181 accagccaga tcctgcgtgg attttcacgc ggtgtgcatg aaaaagccac gttggatgtc
    12241 cccactttgg tgaaggcttt tggcgaagcg cgtgacacag cctataaggg cgtcgtccgc
    12301 cctgtggaag ggacgattct caccgtcatt aaagaagttg ccaatgcaac agaagcagct
    12361 ttagggtcgg ccgcggagcg ggatgcgatc gaaatcctcg aagtggcggt taaagccgca
    12421 gatgaagctg tcaaaaagac gccggaaatg ctccccgtcc tcaaacaggc aggcgtggtt
    12481 gactcgggcg gcaagggact gttcttcatt ttggaaggca tgctcaggca tgtgtatggt
    12541 gagtcacttg aaacacccac catgagcgtc aagcccatgt cggctatgaa tttgcagggt
    12601 gccctcgaag aagtggaaga ggggcaggat tacgaagtgg ttgtggattt tgtgcccaca
    12661 ggtgaacttg acctcggcgc attttatggc cgacttgaag agatgggtac ttccattcag
    12721 gtgggggagg gcgaagggat gtaccgtatg cacatccacg tgcccaccga aaaacgttac
    12781 gagccgatcg attacattat gggtattggc acggtgacaa aggttgccat cgaaaacctg
    12841 cttgcccaga tggatgatat tcaaaaaagc aagcagatcc agtttgctac agttgagccc
    12901 ggacatattg ccgtggttgt ggtttcgccg ggggcaggca tcagccgtat ttttgccagc
    12961 ctgggtgtgg cggccgttgt ggctggcgga caaagtatga acccatccac gcaggatatt
    13021 ttgagttcct ttgaaaacct gccgaccgat aagatcatta ttttgccgaa taataagaac
    13081 atcattctcg ctgcaaacca ggccaaagaa gtgaccgtaa aacaggtggc tgtggtgccg
    13141 actcgcaccg ttccgcaggg acttgccgcc atgctctcgc tcattcccga cggggaactt
    13201 gattctgtcg ccgataaaat gaccaaagct ttgggttcgg tcaaaacagg cgaggttaca
    13261 attgccaccc gcaccgttga aattgacggc gtcagcgtga aggatggaca ggtcattgcg
    13321 ttgttggatg gcaagctggt ggtctctgct gaatcggtgg agcagggtgt gatggaattg
    13381 cttgaaaaag ccgatgccga ccagcacgaa ctggtcagct tgtactacgg cgaaggcatg
    13441 acccacgctg aagccaaccg catcgcagat ttgatcgtca ccaaatacac cgccctcgaa
    13501 gtggaagtac aggaaggcgg acagccgcac taccaattta tcatttcgat cgaataaaca
    13561 agacataatt acccccgaat tcaactcaag acgcagaaga acgctgatcg cgcaatgtgc
    13621 aggcaaaagc gaccgatcag cgttcttctt gttttaactt attttctttt gtaattcatt
    13681 ctcaacaaac cgaaggctgc ctttgccata tccactcctg ccacaatgat ggcgatgatg
    13741 atggcgatcg aaaacgaaag ggtgaaaata ttcagcagca aatcagcgtt gatcgaactg
    13801 tttggcacgc tttggaacga ctcagcggtg aacccgaaga tattgggcgt gcgcagaatg
    13861 gcaaccgcca ctgcaatgct ggcaacttca atgatgatct tggcaattcg agtgagggaa
    13921 gtccatactg cactgcggag caggtaaata tccagcacaa tttcagcgag aaaaacggca
    13981 ttgatccagg gcaggaactt gaagaattcg gctgagaaca tgggaataaa gaccattttg
    14041 tcttcagcaa aataagacat gccaagaata tcggggaaca gattcaaaat ggcaagacct
    14101 ataaaggtga aaacgatctc tgcgatcaag tcactgcgtt tggccgtatc agggtctggt
    14161 tctttggcga gggtggcggg gtcccagtct ttttcgttgc tgaaatcgcc gatttccttg
    14221 tctggaagca cacgctccag aatggcgaag atgaatacga tgttgccaaa agcggtgatc
    14281 gccgcagaaa tgatcccacc caaccctttg ctgacgatgt tcataaaacc cgcacccatg
    14341 aaaggtgtgt cggttgcggc gcggatccct gtcagcacca ataaaacagt tacaaggatg
    14401 ggaatcacga tcttcagtac catcaaaaag aagggaaaca gacgcgggcc gatcaaatat
    14461 ggctgagggt tataggtcgc tgccactttt tgaggtgacc catattcttt cagcagttcg
    14521 atctccatgg cttcactgcg cggctggccc gtttgttcca cacggtcttc cagcatatct
    14581 tcaagcgtgg actttaattc cttctcaata tcctcgcgtc ccttgaggat cggcaggttc
    14641 tttccaactt cagttacata acggtcaatt aaattcattt catctcctta cgccagcact
    14701 tttgccatca cagccacaat gccagcccat tcttttttca atttcgggag gatctttttt
    14761 ccctccgcac tgatgatgta ataacgtcgc gggcgcgctt cttccagctt ccaaaccgat
    14821 tcgagcaatc cctgcgtttc caggcggcgc aagagcgggt agagtgttcc ctgatccact
    14881 tcaagcccct ggtctgaaag caacttgagc agggagtagc cgtattgttc actgctcaac
    14941 tgactgagca cagccagcac gatcacaccg cgccgtaatt cgagggtaac attttccagt
    15001 gattcattgt ttgccatata tcaacctttg tcgcatgata ctgtgtaata cacagtatgt
    15061 caagggcgga atgacacctt cccccattct tatgttaaac tttcaatatg aaaataggaa
    15121 tcgtcaccga ctctacttcc gatctccccg cccacctcat tgaacaacac gagatccatg
    15181 tcattccgtc cattctggtt ttggagggaa aggaatatat ggatggggtc aatatcaccc
    15241 gcgaagaatt ttatacgcgc ctgcccgcgc ttcgcacccc gccgacaacg gctgcgcctt
    15301 cgatcggaga tttcaccgcc ccgtatgagg cgttgctctc aaaaggctgt gaccatatca
    15361 tatccatcca ccctgccgtg aaattaacca ccacgatcaa tgtggctcgc aaggcggccg
    15421 aggaatttcc cggaaaagtc acctgtgtgg acagtggatc gctttccctc ggaatcggat
    15481 ttcagatcct cgctgctgcc gaagcggcgg aatttggcct gcaatccgca ttggatgcca
    15541 ttacctccac tcaaaaacgg cttcacgttt ttgccgcatt ggatacgatg gaatacctca
    15601 agcgcagcgg acgtgtccct ggagccgtag cagctttggg tggattgctt tccatcaaac
    15661 cgatgatcga attgaaggat ggcgaagtaa aacctgtcgg tgctgtgcga atcacacggc
    15721 aggccgacga acgcatcctg aatctgctgc tcgaaagggg agagttggaa cgccttgcca
    15781 ttttgcacac caatgccgaa ccccgcgcca aaaacctgct cgatgatttg atgaatcaag
    15841 cccgaaaatc agttccgcgt gagattttgt tcgtcaacgt cacagcggtg atcggcaccc
    15901 atcttgggcc aaatggcatc ggttttgcag cagtgaggaa atgacacctc cccatgataa
    15961 aattgcccca tgcaaccatc tttggaaaaa ctacgtaaat tttttcgcct ggaacatgaa
    16021 aacaagtacg agaatactgc aattatcggc ggccttgcca aaatgctcga ttattgggaa
    16081 ggggaggccc gtgcggatgg agttgctgaa gaggtaattc aagccgttgc ggctcgtctg
    16141 cgctcttatg agaagctcag cccggatgga agggctgagt cgcttaaagg cttatggaaa
    16201 cggattggag aaacctaccc cgaagctggg caaaagccaa aagcaccacc ccagccttcg
    16261 aaggcgaacc agccgccccg tccgccgcga cccgaaccca cggaccccgc tcaactcgaa
    16321 gcgcccactc caccggttcc gccagaatca aagccccagc caagacctca agcccaagaa
    16381 caacgcaagc cgcgacccac accacctcca caacaacagc gctcccgttt tgagagcgcg
    16441 cccggtgcaa agaccagcca aaccccagcc gctttgaatg cggatatcac tgttcttcaa
    16501 ggtgttggcc caaagcatgc cgaatcgttg aaaagcatcg gcatcttcac actcggtgac
    16561 atgttgtatt acttcccgcg ccgctacgaa gattacagcc agctcaagcc gatcaaatcc
    16621 cttttttatg gcgaagcggt gaccgtgctc ggtacagttc aaagcgtcca cacccgccct
    16681 attcgcggtg gaaagtcgag cattgtcgaa gtgatcatca acgatgggac aggtggactg
    16741 cgtctgtcgt tcttcaacca gccctggctt ataaaccgta tgaaggaagg ggaggcgatc
    16801 tccgtctctg gcaaggtgga ccagtatttg ggacgcctga tcatgaactc acccgattgg
    16861 gagccgatcg aagttgaaaa cctgcatacg gcccgcattg tccccattta tgcgttgact
    16921 gaaaaggtca cacaaaaatg gctccgcaaa ctcatgaacg aagtgattcc gttctgggcg
    16981 cctgccgtgg tggatattct ccccgaaaag atcaaactct ctgcaaggct ggtttcactg
    17041 agtgaagcgc tcatgcaggt tcatttccct gtttcacaga gcaggctcaa ggccgcccgc
    17101 gaacgattgg gttttgatga aatctttttc ctgcaaatgg gcgtgctgcg tcagaaacgg
    17161 gattggaaag atgcggaggc ccgccgcttc tctgtttcga ccgagtggat ggattcgcgt
    17221 ttaagcggcc tgcccttcac tttgacatct gcccagcaaa aatcgcttga agatattcgc
    17281 gccgacctga attcaggcag gccgatgaac agacttgtgc aaggcgatgt tggttcaggc
    17341 aagaccgtgg tggctgcttt gggctcttca gtcgttgtca gtgaaggtgc ccaggctgcg
    17401 atcatggcgc caacctcgat cctggctgag cagcatttcc gtaatttcag caatctgctg
    17461 gcggggccgc agggattttt gcaacagagc gagattcgac ttttggtggg caacacaact
    17521 gaaagtgaga aggaagcgat ccgtcagggg ttggcagatg gttcggtgaa gatcgtcatc
    17581 ggcacacatg ctgtgatcga gcctgatatc ctcttcaaag acctgcaatt tgttgttatt
    17641 gatgaacagc accgctttgg tgtggagcag cgcaaggaac tgcgcagcaa gggtacgaat
    17701 ccacacttgc tggtgatgac cgctacaccg attccgcgct ccctggcgtt gaccgtgttt
    17761 ggcgatctcg atctttctgt gatcgatgaa atgccagttg gtcgtcagcc gatcgatacc
    17821 tacgtgctgc ggcctcagga acgtgaacgg gccttcacaa tgattcgcgg gcagattcgc
    17881 agcggcaggc aggctttcat cgtgtatccc ttgatcgatg aaagcgaaaa gatcgaagcc
    17941 cgcgccgcag tggatgattt cgatgtgctt tccaaggaag tcttccccga tctcaagctt
    18001 gggcttttac atgggcgcat gcgcccctcg gaaaaagacg aagtgatgtt gaaattccgc
    18061 gacagggaat acgatattct cgtttcgacc accgtggttg aagtgggtgt ggatgtgccg
    18121 aattccaccg tcatgctcat tgaaggcgcc gaccgcttcg ggctttcgca gcttcaccag
    18181 cttcgcgggc gggttggtcg gggtgcggcg cagtcgtatt gtctgctcat tcccactcat
    18241 gaggatgcca ccgaaaatga acgtctgcag gccatggcca agaccaacaa cggtttcgaa
    18301 ctggctgacc tcgacctgaa aacacgcggc cccggagaat ttctgggaac ccgtcaggcg
    18361 ggctttggca cccaattgaa aatggccagc atcaccgatg tatccttgat cgaaaaagca
    18421 cgcacacagg cgcaagccct gtttgaacgc gatccgtatc tgcaaaatcc cgaacacgcc
    18481 ctgcttgctg aagcctttga gcgcttctgg ggcacaggaa aaggagatgt tagttagctg
    18541 aattgtccaa gggtcaattt gttgcgtggt cgcatggtca tgctcgaatt tccccgacga
    18601 ttcaaaaatg caactacaaa accaccatgg ttagagcatt gttccccggt acattcgacc
    18661 cgatccacta cggccacatg gatattgcct cccgcgcatc gaaactattc gatgaagtgg
    18721 tcatggctgt gtacgacaaa cccttgaaaa gcctcatgtt ctcacctgag gaacgcattt
    18781 cactggtcac cgaggcattc aaagacaacc caaagataaa agtgactggc tacagcggcc
    18841 tgacaattga tttcgcccgc aagatccaag cccaggtcat cgtgcgcggc ctgcgggttt
    18901 tttctgattt cgaattcgaa ttccgcatgg cgctggcaaa tcaacgcctg gcaaaggata
    18961 tcgaaactgt tgcgctgatc accgccgagc aacacacctt tctttcctcc agcacggtac
    19021 gtgagatcgc catgcttgat ggtgatgtct ccagcatggt gcctgcccat gttaaatctg
    19081 ctttgcatgt caaagtgatt aatatgggag agcaatctcc acccaacgca ctccgcgatt
    19141 aatttgtgct agaattggaa aagtaattct cattcatcag gaatccgccc acgagaggcg
    19201 cactatggac attctgcaac ttattgaccg attggaagaa ctcttcaacg cagcaaaagc
    19261 tgtgcctttt acgcacaacg tcattgtgga tgaagaccgc atgttggaat tgatcgacca
    19321 gatgcgcatc gccatccctg aagaagtaaa aaaggcccag cagatcatgg cccagcgtga
    19381 ccgtgtaatg gcccaggccc aggaagaagc caaccgcacc ctgcagcttg cccgcgataa
    19441 agccgaccaa ctcacccaaa aagacatggt cgttcaggaa gcccagcgcc gtgccgatca
    19501 gatcatcaac caggcacgcg gtgaagcaga agccacccgc gccgatgccg ataactacgt
    19561 ggtggatacg ctcatgcaac tgcaagacca gatcgcaaaa atgagcagcc aggtcagcaa
    19621 cggcattcgc atggtgcagg aagaccaaat gcgaaaatcc gtctcatcag aaaaagtgga
    19681 ataatcaaat tgaacagccc cgccaaaatg gcggggctgt ttttgtttat atctggaagt
    19741 gcgccacagc gcacccgcat cactgctgga cctgcaacct gagtgctgtt ttcccaacat
    19801 tccccaagct gacgaatatc ccagtaccca aaaccaacag cccaatccca ataacggtca
    19861 tggcagtatt gaatgcatcg gcatagttaa tgccatccca tgtgtgggtg ggtcccattg
    19921 tatattggat ggtgagtgtg aaaatcccca ccacaaaggc cgccgcacca cccgcttttt
    19981 tgttatcact ggctttcgct ttggaaaaat ccacgccctg ccagatcatg gggatcttga
    20041 aaagcaggaa gatgaccagt gtaagcagtg tggtgtacac caccgcatcc acaggcatgg
    20101 actttccacg taacatgcgc gacatgtaga tgtgaaatcc gccgaccaat acgcctgtga
    20161 tcaacgaata cagactcatg cggtatgcat ccactgtgcc tttgatgagt ttgatggccg
    20221 cccaaattcc ccaaaccccg atggcaatcg ttccaagcat gaagaggata tacagccatt
    20281 ggtatggaat caaccccacc atgctttcat atttttgcgg gaagaccgcc gcgcaaaacg
    20341 tccccgcgcc gccaagaaaa gtaaacccgc ccgtaagcgc catcagtacg atcccaatga
    20401 accgcaggga ttttgcaaag aaattatttg cagacattgt gtactccttt tgtgaaaaaa
    20461 acaggtcacg cttcatgcgt gacctgtacg ttggttatcc aagtttgaaa cctgctacga
    20521 agagtgctgt tgtcagcagg agcagcatgg ggaacaatct catgatcgta tcccttgata
    20581 agatcggctt tcctgttttc agaaaggaaa gcaacaaccc gccgatgccg atcaaagctc
    20641 cgccaatgcc cacaagggag aaaagcggtt tcacctgtcc ctgtgctgca aggatgatgg
    20701 gaagcaggaa aatggtgagg cctgcaatgc cgtgggtgat ggcaatgaca atgacaggca
    20761 gcttgttttg catggcaatg gaacgggtta ataccacggc gagaaaactg atgatggtga
    20821 agatcaggta aacggtccgc agggaggcga ggtgctgcca gatgaggccg agtgacaggg
    20881 cgagtggaat gatcgttgag atgatcacaa cgatgggcgc atccaacact tcaaacccga
    20941 ggatgatgag cagaagccct gccaccagga tgacaccgaa agcaatggtg taggctgtga
    21001 tggaaacact atccatctgg tcaatgccga tggcgatctg gtatgcggca agaaaaccag
    21061 tgaaaagaag cagggtgcgg tcgagggaag acattttcat tgggcaatat gcagggcaac
    21121 ataaatgttg cgttacggaa tgatcagcaa catcgcgaac aacatgtaca tggatgcgta
    21181 tttgaacaag ctgaagttga cgcggtctga tgggcgaaaa accgtggtga tggcaaggaa
    21241 gaacagaccc gcgctcagca caatgatgag tcgcagcaca cctgctgtga ccccgatcca
    21301 gaccgatgcc cagcccatgg tgagtgctgc aaccacgctg gagaatgcga tcgcaaatcg
    21361 ggtgaccgct tcgccgtagg tggaggggaa ggtgggcaca cctgccgatt gatagtcatc
    21421 gaagaatttg aggctgaagg tgagcgtgtg cgtgggaatc caaaacaata cagcgcaggc
    21481 cagcaaaatg ccgatgacat cgatctggcc gacggccaac acacggcctg cgaggatggg
    21541 catggcacca gagatgccgc cccacacaat gctccagcag gtgcggcgtt ttagccacat
    21601 gctgtaaacg accacgtcga aaaataaacc tgcaaatacc acaaggccgt ataaccagtt
    21661 gaccatcacc gcccagccaa tgccgatgac cgacactgcc atgccgaggt tgaagacctc
    21721 ttcacgggtc acctgacctg cggaggtggg gcgtttgtgg gtgcgtttca tctttgcgtc
    21781 aatatcctgg tcccaccaca tgttgaggat ggtggagcca gaaatggcaa ggaagagact
    21841 gggcagcatt tgccagaagt gaagccagtt tacggtggtg tgcgcactca aatagccagc
    21901 cacgcctgtg agcaaaagca ggctggtctg cagagatttg atgagcggga tatacagacc
    21961 gagtttggat ctgatcgtgc tgaaggacaa ttgcgcttgc atgagatact cctgcgatgg
    22021 atgggaacaa gtaaacaggg aaccaaatta cagagcgcaa gtatacctgt gtacatgtgt
    22081 acttgtctac ttgtatggtt tgttactcat cccgcgcaca tcgaaacccg acacccgggc
    22141 taaagtaagt gggtgtggcg ttattgcgaa cccaaatgcg caggtccatg tcgtaggtgt
    22201 cttttgaacc gccgcgcata aaacggtaat gcattccttc gtatacatca cccgtccatt
    22261 gccagacgtt gcctgccatg tcgtacaggc cataggggga ggctgagtca atggtttgga
    22321 agccgtcgta aacctgccca ttgtagaaac caactgggct ggtgcgtgaa ccaaaagaag
    22381 ccatgtcttc gaacgggtcg cggctggagt agaagttggc atggccgcgt tgaatttcat
    22441 caccccacgg aaacggacgt tcatcggtgc cgcgtgcggc tttttcccat tccaattcgg
    22501 tggggaggcg ggtgccaaaa tattggcaat atccccatgc tccaaaccag gtaacactcg
    22561 tcatgggatg tttttcgtac ccagattgaa cggtgaatgt tgttccatca aattggattc
    22621 tctgcgaggg atcatcaagc ggaatgaaga gccaatcgcc cgcttcgatc ttctcctcgt
    22681 gcttgacccc gtggaacgtg tcgcctgggt agaacccgac aattttgccg ccgtccattt
    22741 ttatgctgcc atctgcaagc gccgcgttga gaaaatctgc atactgtgcg gcggtcacat
    22801 cagtgaccat gatctcgtag gcttctgtgg atgcggtttc atcgaactgt ccgtggtgga
    22861 attcacctgc tgggatctgt gcccaggtgt tcgggtcaat gcctgtttca aaggtgggga
    22921 tcggagcgtt caaatccacg ggggcgcagg atgccagtaa cgccgcacac aggcaaaggg
    22981 tcagagtgaa gcgtttcatt tgtccacctc ctgtgcgagt tcgtgatcca gttcctcgat
    23041 ccaacgccct ttgttcttga agaggtcgat caaacgatga accattatga tcgcaaggat
    23101 gaccagcagc atgccaaggc caaaatcaat ggcatacatc aacccgttga caagtggttg
    23161 ttgggcggct tctgtaacaa agagccgctt catttcaaac aaagtgaccg cgccaaaggc
    23221 gatatctgaa agaatgatga tgccccagcc ataccactgg cgcagcctgc ctttgaagcc
    23281 gttcatatct ccataataca ggaggatgat ggtggcaatg atggcggaaa gcacatgcca
    23341 atgaccagtg agttcaatgc gttcttcacg tgcaggccac acacgaaaga tttcatcgag
    23401 tttgatcgcc atgaaaatac cgaggccgct ggtggtgaaa ttcatgaaca gcatttgcca
    23461 ggtgggaccg aatttgagcg ggtcacgtaa tagggcgccc actttctgac cgaaggttgg
    23521 ctttgtgagg tgtgccgtgc catccttgat gagcttggcc cagctccaga tcagcaggaa
    23581 gagcgccgcc agcatggacg gggttgcgcc gacgtagatg accatgtgtg ccacaggctc
    23641 gagcggggta accacacccc acacgcccag gttgagtaca atggttccaa ggatcattaa
    23701 tggcatggcg gccttgtgcc acgcgccttt gaaattcaac cagcgcccga tgatgagcgt
    23761 gatcatgatc ccgatcaatg ccagcatgat gtgcaaatga ccgatgaccg aatattccat
    23821 cacggttttt tcaggcaggc gaatgatgtt ctctgcgaga aaggtttcaa agccatggcc
    23881 gtaatatgag cctggcaccg cgccaaaaag agacgagatt acggttgtga ccgcagtggc
    23941 aaaaaaggcg gtccgctcca ggtccagtcc gcttttggtg tgggcgtaac ttttatcggt
    24001 gacatgatat tcaggattcc acggccagag cgccacgatc aacatcaggc ctgcaaagaa
    24061 gatcagcgaa agccctgtaa tgtataaccc gtgaaaagcc caattacgcc caaaatatgc
    24121 aaagcccatg ccaaagacca tggtcaggat gtacccaact gtgatggtgg cattgatcgc
    24181 ggttttgaaa aaaggcttca ttttgatcaa ccctgttacc atgtaggttt caatggcaat
    24241 gatcgccatg gcgatcgaat gatacaaaat gatgatgcgc ccttcgcgtt cagcctgcac
    24301 gatcttcatg ccaagcgttt tgatgacgat ctcgcgtacc cccatctcag ccatcggccc
    24361 tgaaagcatc ccccatacgg cggtgacaat tcccaccatg gcaattgcca caaggatcaa
    24421 tcccttggtg gatgtgaaaa ggtagttaaa tctttctttc atagtttttc ctgnnnnnnn
    24481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacttatg ccctttttgc aaaacccagc
    24601 acgtatgcac ctgtcatcaa aaagagcagg ggagtgagaa tgagcgtcac aaactctggg
    24661 ctgaagaaaa gcaactgttt acccagagtg atgaacgcca gcgcaatacc gccgaggccg
    24721 atcaacaacc cgccaatgcc cacccaaaag aaaccctttg gcgcgccctt cgcataaaac
    24781 gggccaatga agatcaccaa ccccgccacc ccgtggaaca gcggaaccgc gatctttttc
    24841 agcaaatcca tgccgccaat gctggtcact gcaatcgcca gaaaaccgag cgccgcaaac
    24901 catttgaaat attttttcca ggcagggaag tattcttcag caagccccat ggaaaccccc
    24961 aacggaatca aacttgcaac ggtcagcacg taaggcgagg aaagaatgcc gagtccgaaa
    25021 actatgagca acagacccga aaccaaaagc actgcaaatc ccataaggta atacaggtta
    25081 tgcagtgcct tgtgcaactg gtagcgctgg tagaaatacc agcacaagta ggccgcgagc
    25141 aggcccgtca aaagcaggaa gacgatgtct ttcatggtca ctctccttgt tataatgtct
    25201 tgttgactat gtgaattatt gcacaaatga aacctgcaaa ctgtaactaa agtcacattg
    25261 caggatgaaa ccccgcttct tatccaaacc tgcaaactgc ctctgtttcg aaagagaatc
    25321 agcaccgtga gagaccaccc taaagaacgg ctatattcag taacctcaaa gggtgttttt
    25381 gtgtttatga actgtataat ttgaattgca gatgaaatcc ttattccgcc ccatcttttt
    25441 ctttgacccg tccatctcgc gcagccttca agaatggcgc gagctggttt tggcggccct
    25501 tcttcgtatt gtcctgttga tttggatatt gtctcttatt gcgggggtga acaatgtctt
    25561 caagtcttac attctggaag tagataaaac ctccaatccg ttgttacagg cagggattgt
    25621 attgtcggtt tacctgctgg cgacttttac acttgtgttg gttacctttg ggaaaaaact
    25681 cccatataac cttcgggcct ggggattgct tttttctgtt tatatcgttg gcgccattgg
    25741 gcttgttttc tcgtctttaa gcggcgatgg gcgggtcatt ctttttgcat tcattattct
    25801 tgccgctgtt tttttcgacc ttcgctacag tgtgccagct tttgccattg gtttggctac
    25861 catggtaatt gtcggctatt tgcaggtaaa tggcattctt gtgatcccgc ccgagcgtca
    25921 actcatttca acggattcct cttcgtggat cagtggggtg gtggtttttc tcaccctgaa
    25981 tattgccgta ctcatttcca ttaccaacct gctgcgcgtt ttgggcacga atttggaaaa
    26041 atcgcaggaa cttttgcagc gcgaacaacg actttcccaa attctgcgca ctgtgacagg
    26101 aattaatcag cttattgttc gtgaacagga tccccatcaa ctcctttctg aagcctgcat
    26161 tttgcttgtc tccgggcagg gatattcata tgcctgggca ggattgcttg aatccgacca
    26221 gatgacctta aagcttgcag cccaagctgg agataaaatt gacccagctt tgttcaatgt
    26281 acgtattgat gaattgaatg agcgtgggtt agcctgtgct gccaatgcaa taaaatcccg
    26341 ctccttcttc cgtgtagacc caattaaagg ggaggatgat ccctgcaagg gttgtccgcg
    26401 ccgcatcgac aacccggagc gggttggggt ggcattgcct cttttacgtg aagaccgtgt
    26461 tctgggcgtt ttagttgttg atcatgcagt accggaggcg gtttttgaag atgaagaaat
    26521 tcatctcctt caagacctgg caaatgacct ttcctatgca ttggaaaaga tcgaagtcaa
    26581 tgacaggttg caggtatacg cccgtcatca ggggattttg aacgccatta cccaggtcgc
    26641 tcttgaaacc cccagccttg aggccatgct tcaaaaactt attgaaaatt tggaaggcat
    26701 gtttggagca gagggatgtt atttttcact ttggaatgaa gaaacgcaac aagtggaaaa
    26761 atttatcagt tccgatattt ataaaaacat cctgggaacg cgcccgaatt tgaatcccgg
    26821 tgaaatgacc ttcacccagt ccattcttga aaatcggcag atattggtcg tggacgatat
    26881 catgaacagc ccgttcatca gtccgcgggt tgcttctttg tttcccgttt tttctgcggt
    26941 gggattgcct ctcattgcaa atgaaaagaa actcggtgcg ctcatctttg gttttcgtga
    27001 atcagcaccc cctttgtctg gagatgatat tgccctggct gaacagaccg cccgccagat
    27061 tgctttggca atgttgaagg tgcgccttga tgaagatacc cgagccaaag ccgctgaact
    27121 tgaacggcta tatgcggctg ctcaagatat gtcctccagt ttgctggatg cccccgaatt
    27181 gcttgaaaaa ctcgctcatc acatgaccga ggcgttgtca gtcaccagtg gcaatatcat
    27241 ggacatcaat ctcgttactg ccaccatgca ggttgtgggg gagtattgga gcgctgaagc
    27301 ttcacccagg gaagtgcatt cagatttggg cagagttttt cagaacgagg ggtatggcag
    27361 catcataaag tccatgcttg cgggtgaagt gctcattatg cattccgatg atgaagagat
    27421 gacccccaca gagcgtgagc aatttgctga atatgggatt tgctccatga tgttcgtgcc
    27481 gatcctggcg catggacagt tgttcggtaa catcgaaatt tgggaaagcc gtcgccgccg
    27541 tgagtttagc caggctgata ttcggctggc gaaagccatg gctgggcatg cagcctccat
    27601 cattgaaaac gccaaacttg taaatgcggt ccgcgtttct gagacccgct accgtaattt
    27661 ggtggaacag gcttctgatg gcattttcct tgttaacccg gaaaaaatga tattggatgc
    27721 caacccaagc ggtgcagcca tgctggggta ttccattgaa gaacttctgg ccatgagtgt
    27781 ggatgacatt gtcattgctg gacactcgaa cctgcttatt gatgaacttc gaatgggaag
    27841 gtccatgatc gttgagcagt atgtccggtg caaggatggc agtaccctgc ctgtggagat
    27901 cagcgccaga atgctgccga atggatattt tcaaggaatc gtgcgcgata ttagcgaacg
    27961 caagcaggcc gaaaaggcgc tggcagagcg tgaggcatac ttccgtgctc tcattgaaaa
    28021 ttctgctgaa ggtgtcgcga ttctggatga ggaaggggtg gtccgttaca ttgcgccttc
    28081 agatgagaag ttgacaggat atagtgcaaa caacgttcaa gggacgagcg gctttcaata
    28141 cattcatcca caagatctgc ctggggtgat gaaaatattc aaagaaggcg tggcaactcc
    28201 gggcgcggtt cgaacgaccg aatatcgtct ccgccgtatg gatggggagt ggcgctattt
    28261 cgagatcacc ggtcacaata tgttggatga tccacatatt catggtgtgg tggtcaacta
    28321 tcgcgatgtg accgagcgca aactggctga acaggccttg caggaaagtc gttcaaggct
    28381 ggaagcgatc atctcttcgg cgttgaatgg aatcatcacg atcgatgaga gtcagcacat
    28441 tattctattt aacccatctg cagagcgcat tttcgggtat gcaacttccg aggttattgg
    28501 acagccgctt tccaaattga tcccagatcg ttaccatgct tctcatgggg tgcatgtgaa
    28561 ggactatgcc aaaagcggaa tctcagcccg tcaaaaagga ttgcttgatt ctttgtttgg
    28621 gaagcgtgcg aatggtgagg aatttcccat ggaggccttc atctccaatt cgcaggtggc
    28681 aggcgaaaga tttttcactg tgatctttca ggatatcacc gaacgcaggc tggccgagga
    28741 tgccctgcgt gaaagcgaaa gaaaattccg tgctttggcg gggaacatcc ccagcacagt
    28801 gtatcagtgc aaaaacgatg aacgctatac agtcatctat ttgaatgatt cggtcgaaat
    28861 gctcactggc tatcccaaag aggattttct ggaaggtggt ctgtcctttg tagatttgta
    28921 tcatcctgaa gacgccaaac aaattccaat tccgtccttt gtaggggagg atacatcctc
    28981 cagcgacctg tcttttcata tctcttatcg catccgccat cgttcgggag attggcgctg
    29041 ggtggatgag tggggggtgg gggtcttgga cgataggaat gcagtgcagt accttgaagg
    29101 tgtgatgatt gacgtcactg accgaaaaaa tgctgaagag gaacttcgcc gacatgccat
    29161 ggaacttgag acactctctg ttgcctcttc agctttgcgc actgcccaga acgtgtcaga
    29221 gatggtaccc atccttgccc gtcatgcctt gcaagctgtg ggtggaacct ttgcatccat
    29281 attcctgctt gattccgaaa cccaggattt tgtttcacat ggctggtatt cggtggatcc
    29341 cggagtcaat ttcacattgg ccgacgagtc ggaagtgagg cataagcccg gtaccggtat
    29401 cactggccgt gtcgccttga caggtgagat ctacatcaca gaagatatcc aaaaagattc
    29461 ggttatcacc atattggatg gtgaacggga acggctcatg accgtacatg gcggaatctc
    29521 cctgccgctg cgcacacagg aaaagatcat tggcgtcatg catgtctggt catcggagcc
    29581 gcgcatcttc aacgagaccg atatccgtct attgaccgcc cttgcagaaa ataccagtaa
    29641 cgccatccat cgtgctgcac tctttgagca aaccctcaac catgccagtg aattggccac
    29701 agcgtatgat aatacccttg caggttgggc acgtgccctt gaattgcgtg atgaattaac
    29761 cgaaggccat acaaggcgtg taacagaatt aaccatcaaa ctggcgcagg cgctcaacat
    29821 ccccgaagct gagatcgttc acattcggcg cggcgctttg ttgcacgata ttggcaaaat
    29881 gggcatccct gattccatcc tgcacaagcc cggcgcattt accgcacaag agcgcaagat
    29941 catgcaacag catactcaat atgcctatga tatgctggct gccattccct ttttgcaggc
    30001 cgcactcgat attccttatt gtcatcacga acattgggat ggtgcaggct atccgcgcgg
    30061 attgaaaggc acagagattc cgttggccgc acggatattt tccattgttg atgtgtggga
    30121 tgcgcttacc tctgaccgtc cctaccggga agcctggtca aaggaaaaaa cccgtcagta
    30181 cattctcgaa cgttcgggga aacaatttga cccgcagatc gttgaagcgt tctttgcgtt
    30241 cgtagcgcaa agcgattaaa tcatcacatc ccgcgccagc agatcctgga tcgtttcccg
    30301 tctctggatc aggcgcgcat ttcccttttc aagccacaac actgcaggcc gcctcgaacc
    30361 attgtagttc gatgacatgc ttagctgata tgcccccgaa gcagggatgg cgatcaaatc
    30421 accttcgtgg atatcccgca tgggcaggtc ttcaatcacc acatcccccg attcacaaaa
    30481 tggccctgcg atggagatcc gtccgcttcg ctctgcgccg acgcctgata ccggtaaaca
    30541 ggaatatctc gccccgtaca tcgcatggcg cggattatcc gtcatgccgc catcaaccag
    30601 aacccatgtt ttttcatcgc gctttttaat ggcacctgcg cggtacacag acacacccgc
    30661 ccgtgcaacc aaactgcgcc ccggttccag atgtaaatga gggagggtca atccgcggct
    30721 ctgacagccc gcaaggacat tctctgcgat cacacgcaca tactcatttg tatcaggctg
    30781 gggaagttca tcttcatgat aagccacccc ccaaccgccg cccggactga agtgccaaac
    30841 atcaggaaat ccgatttctt ttgcaatatc aagaccaatg ccgatcgctt ctttcaaagg
    30901 acgggcatct cgaaaattcg acccctgatg gaagtggatt ccattaatgg gcaggtggtg
    30961 ttgcctgcat atatctgcgg cttccattat ctctgtgcgg gtcatcccga atttgctgcc
    31021 atgttgaccg gtctgggtat gggcatggtg ggtttcaact gcaaggccgg gcagaaggcg
    31081 aaaccaaagg tttgatgcaa cagtggaaac agccgcaata ttttttagtt cggtcaggtt
    31141 gtctacaacg atcgtacctg catgctgaag cgctgatttt aagtcggcct gtgatttgtt
    31201 cacaccatgt acaaggatgt gctcacgcgg tacgccgcct gcctgggcaa aggcgatctc
    31261 cacctcaccg gtgcaatcca cgtacaactc atgtccatgt gcccattgag caatggctgt
    31321 acaaaggtag gctttgccag cataagtaac agaaccaggc ccggggtagt gcgaagcgag
    31381 cgcgttctta tattcaaggg cggcatcatc cattgttttt tggtcgtaca gatacagggg
    31441 ggtgccatat tctttcgcca atagttcaag gtcgtgcccg gcgatggtaa ggcggttttg
    31501 ctcgattttt gctgaaacgg ggaaaaggtc aattctattc atcattttta tagaggttgc
    31561 tcgcaacgaa gatttgattt ttgccgagac gttttgccgt gagttcggca tggtctgcag
    31621 caaggaggat gccagccagg ctgtttccat ctttaggaaa ggtggcaacg ccgatgctta
    31681 aagtgtaacc aggtatcacc ttgcgttctt catggatctc agctgccttg gcacgggctg
    31741 tattttgcaa gcggcgtgca aattcgagtg cgccgtgttc atcggtgttg ggcaggatga
    31801 tggcgaattc gtcgccgcca tagcgtgcag cgacatcata ctttcgcaag ataccgcgaa
    31861 tcaggtctgc aatggcacgc agacgggtat ccccggcggg atgcccccat ttgtcattgt
    31921 attccttgaa agagtcgata tcaaagatga tcagcgaaag cggatgccca agcctcgtgg
    31981 aacgctctac ttcggcggtg agatactctt ccaatgcgtg gcggttggag aggccgctga
    32041 cggcgtcggt cattgccatg cttttgactt gtgcatgcag gcgggcattg tccactgcca
    32101 gggcgatggg gcttgagagg gtggttagta attcaacatc ctcccgtgtg aggatgtcgt
    32161 ttgcatcgcc ttccacattg agcgtaccaa ggacaacatt ttcctttaat aatggcacac
    32221 agatttcact cctgaccgtt tgatctgcac ggaggaaatc gggttcccgt gatgcatctg
    32281 ggatgaattg aacggccttg gttttgatgg tacgtccgct gaccccctga ctgatgtgga
    32341 tcttatgaat gatcatctcc aatggataac cgacctgcgc gccgaggtaa agatattcat
    32401 ctttcaataa ataaatgctg acatgactgt aaccaaagga attttttaat tcacgaacaa
    32461 cggtttcaaa gatggtctcc aggtcgagcg aactgataac aacacgcgag acagcctgaa
    32521 ccgcagacat aacccgcagg tgttcctgtg cctttgaata aagcgccacc cgttgcaatg
    32581 aagcggagat ttgatttacc agagtttcca gtgtggttat atcttcagaa gtgaccgcat
    32641 cagactcgat ggtctctaca tacaaagctc catataattt gttttcgatc aagatcggca
    32701 cacatatggc agagccgtga tgttcatcgt tcgagaaata atagggatct tcagcgacat
    32761 tcttcgcaac atatgtcttg cgggttttgg cagtgtgacc gatgattcct ttgccaaatt
    32821 cctgcctgaa accggggtca tagccgaaat catgcgtgcc ggaaatgacc ctgatctcca
    32881 tcatgttgtc gggcgtcaag accgatatgg cggcttcgcc atacccaaag cgatctacaa
    32941 ccgcgtggat ggagtgttgg agaatttcca tttcatcgga actgcttgcg atctgcctgc
    33001 caacttcttc aagcaggcca agatgggccg agcggcgaaa ttccgattcc agcagttcct
    33061 gttcctgtgt aatatcgtgt accaccagca cacgtcccaa taattcagca ttgttcttgt
    33121 gcaaggggat gaccgaggct tcataccagg tccgcacacc atctgagcgg ttcagcctga
    33181 atttgatggg ttcattcgtg atatcccatt tttccatgaa catttccgca ttgggaatgg
    33241 cttcgaagat gttcatgcca acaatctggc ccttggcatt catccactct tttgcggctg
    33301 ggttcaggtt ggtgatgcgg ttttgtgaat cggtcacgat gatcgcatcg tgcaggtttt
    33361 ccatgattac cggcgcagcg attggaaaca ggttgtatag cccaaagcgg aaaaatccga
    33421 aggccaataa tggagccgat agagcaaaag acaggggagt ggggtctatg tggttgttgg
    33481 agggttcagc gacgaaggtt agattgacga tcaacgtaat aagtgccccc aataacatga
    33541 tccccgtttg tatgcgaaag gtttttggcg aacggataaa agcataaatg taaacaagaa
    33601 accccatgcc aatgaaaaaa tatgcaatga ccgtgaagaa ccagaaccct gtaccaaata
    33661 tgacatgcag gggaccgatc tcatgcccgg gggggatttc gagcgacttc cagatcagac
    33721 catgaatttc attagtcaat cctagaaaaa aggaagccaa tcccggaagt gtcattaaga
    33781 atacccgtcc gcgctttgac caccacggcg caagccccgt gtatcgcagg gcaaatccca
    33841 accacaatgg cggtgagata cccataccga gatacatgat ttttctacat aggatcttta
    33901 aggggagaga aggagctatg atttcacaaa gatagaatgc agcccagatc atcatggaca
    33961 gcattaagaa tccaaaagtt ctggcaccgg gtctctccca attcaggaat gcgccccccg
    34021 caatacccaa ggttaggagt accagaaccc caagataaaa tgagaacagt cccaggtcga
    34081 tcagcattaa gaggtttatt cgctaaagaa caagctccac cacacctccc aattttgcat
    34141 tggggtgggc gtggggatcg gagtggagac gatcacaggt tcgctgcccg gttgaccaat
    34201 tggtatgaca ggctgatcgc cctcctgaac gtaatgccct tgtgtacgag cccattcatc
    34261 cacagctgca gttgactctg cataggccac ctgcgtattc agtgcgacct gcgtttgcat
    34321 ggcctgtgtg gcgatggcac gcacctgatc gcgttggtcg ttcaggcggt ttagttcttc
    34381 gaggcgtgta ttgaattcga tcaccaccaa gatcaacaca aaaatgccaa aaaaagtggc
    34441 ggcacgccga aggctgatgg gaaggttggg catgcctgta tcttaatcgg atttgcccgg
    34501 cttggcaaga aaagccccct ttatggtaca atctgccccc gcagaacgta gtacccttcg
    34561 ctctgcatat ctcactccca gaaagggctt gaacgaaatg aaagtaatgc ttgttaaaga
    34621 tgtttataag ttgggtcgcg ccggggatat caagaaggtg gctgatggtt atggccgcaa
    34681 cttcctcatc ccgcagggtt tcgccgtatt ggcaaccgaa ggcgcactga agcaggttca
    34741 aaagatcaag gctcaggccg agattcgccg caccgctcaa aacgaagaac tcaagggcct
    34801 cgctgaccag atcaaagccg tcaccctcac ctttgccgcc aaagctggcg acaccggcaa
    34861 attgtacggc tccatcacca cacaggatat cgccactgcc ctcaccgaga aggtccgctt
    34921 tgaggtgaag cgccaacagg tggatattca gcccatccgc aaccttggcg aatacaacgc
    34981 acacgtccgc ctgaccatgg acctggttcc tgaagtcaag atcatcgtcc accgcgacgg
    35041 cgaatctgcg gaacctgccg ccgaagaaga aaaggccgac aagaagaaaa aggccaagga
    35101 agaaacagct gctgaagaag caccagccgc tacagccgaa taaaagaccc aaatatcacc
    35161 ggcgcaagtc ggtgatattt ataattccct gaagcacagt aaaataaaga catgtctgat
    35221 tcgattggtc agcggttgaa aaaagaacgc gaaaaccgtt tcctcacgct cgaaagagcc
    35281 tctgcggaaa ctcgtattcg cattgtgttt ttgcaggccc tcgaatcgga tgactattca
    35341 gtaatgcctt ccgccgcaca ggggcgcggt tttctgcgca actatgcgga atacctcggc
    35401 ttgaacattg acgagatgat cgccgagctt caaaagaaag ccgctgaggc tgcacaacaa
    35461 caagcagtgg atggtcctct gccgcaggtc aatctttctg aaacggaact ccctccgctt
    35521 accgatgtga aagaggaaaa acccgcgcgt cctttcttgt cctggctggg tcgccgtcca
    35581 aaagcggact ctgacactgt gagcgaatcc accgaacccg caccagaaga aacgcctgct
    35641 ccggtggttg aacctgttga acctgctgcc ccgcttgaaa tccctgttgc acctgttttg
    35701 gaaactccac cagttgagcc cgtagaaaaa cccaagacac gcggccgtaa aaagaaaaaa
    35761 acgccagccg atgaagtgga agcggaggca gtgcctgttc aacctgaacc cgttcaggag
    35821 gaaatcgctg ctgttcaggc ggatgtgacg gagagtcaac tcgatgcgct gattccctcc
    35881 agcgaagaga atcaagatga ggtgaaggaa gaggcgaagc cgagtctgct ttccagattg
    35941 ctgggaatct tctcatcccg tcctgcaaaa tcagagcctg aacttcgtcc tgttcttgaa
    36001 accagaccac aacaaacggt tccgttgcag cctgccgacg ttatctttgc tgagatcggt
    36061 cagcaattac gttcgcgccg tgagctgatc agcctgactt atgatgaggt ggaacgccat
    36121 acaaaattgc gcgccgcttt tgtgaaagcg ctggaagaag gcgcgttgga tcgactgcct
    36181 tcacccgtgc aaacgcgcgg catgctggct aattacgcca ccttccttga ccttgatgtg
    36241 gatgctcttc ttctgcgttt tgcagatggg ttgcaggcgc gccgccatga gaaatacgct
    36301 gaaacaccgc gtgaaaaaat ccaaatggaa gtggtgactt cgatgcctgt tttgcgaagt
    36361 ttcatcgctg gtgatctgat cttcggtgtt ttgattattt cagtgctggt ggctttgggt
    36421 atttggggtg tggggcgcgt ggtcacttcg caggatgaag ttgttgccga ggtcactgcg
    36481 ccttcgatcg tgcaggtgtt gggcggcact ccgttgccca cttccatctt gaatgccaca
    36541 gccaccattg aagggacacc cgccgaaacc cccacagtgg atccgttgtt tacgcccgag
    36601 gtgccaaccc cggtttcgac cgcaaatatc tcggtgagca tttttgctgt ggagcgcacg
    36661 tttgcgcgcg tttctgtgga tggggaggtg gtctttgaag gccgctttgc cccgggtgaa
    36721 acaaaattat ttgaagccgc cgagcaaatt tcgattttga ccggcaatgg cgccgggctg
    36781 cgtgtgacct ataatgggcg tgatcttggt ttgcttggaa atattggcga ggtggtcagc
    36841 cgtgtgtatt tgatcacggg tgtggcagaa cccaccgcca cgatttcgcc gacgcccacg
    36901 gtcactgtgc ctgcaacgat cacacctgtg ccatcgtcca cgccatccat taccccgacc
    36961 gttacacctc ccgatggcgg gtaattagca tatttgattt tcgccaggaa taggtttcgt
    37021 tttttttatt cgttactcaa gtcatagtca ggaaaacaca tgtcaaaaaa tacatttcac
    37081 ttggtttcgt tgggttgtgc aaaaaataca gttgattctg attcgatggc acagttattg
    37141 atccgtgatg gatatcgcgc gatggatgag cctgacaagg cggcggtatt gatcgtgaac
    37201 acgtgcggct tcatcggccc ggcgaaggaa gaatcctatc gtgtgttggg tgagcttgcc
    37261 aaatccaagc acagcggaca ggttttgatc gccgctggtt gtttaaccca gcgctacggc
    37321 gtggaggtgg ctcagaaggt gcaggggatc gatggcatcc tcggtacgcg ccgttggatg
    37381 gatattgtgc aggttgtaaa agaagcacgc aaaggaaaac accccgaacc gatctatcat
    37441 ttgcccgaag cggcgacggt cggtgtcgat gagcaggatg ccttgcgggt gagtgtggcg
    37501 ggagccagcg cctacctcaa agtggcagat ggctgtcgcc gtccgtgtgc attctgtgcc
    37561 atcccgttga tcaaaggtac ggcagtttca aggcctgtgg atgccattgt taacgaggcg
    37621 cggaccttgc gcgataatgg cgttcgcgaa ttggtgctga tcgcacaaga caccaccgat
    37681 tatggtcatg accttggcat gaaagatggt cttgccgttt tgctggaaca attgaccgac
    37741 tctgtaccgg atatggactg gatccgcgtg atgtatgcct accccggcta tgtgaccgac
    37801 cgcctcatcg aagtaatggc aacccgcaaa cagattttgc cttatctcga tatgccgctg
    37861 caacatgcac atcccaaaac cttgtatcgc atgaaacgcc cttcgaatat cgaatgggtt
    37921 cataaaacac ttgggaaaat gcgggcggca attcctgaat tatccatccg caccactttc
    37981 attgtcggct atcccggcga aactgatgag gaatatcagg cgttgtacga tttcgtaaaa
    38041 gagattcgct ttgaccgtgt gggagccttc cagttttcat tcgagccggg gacgacatcc
    38101 gcgcctctgg gcgacccggt gcctgcttct gttaaacagg aaagatacga gcacctgatg
    38161 gagcttcagc aaaccatctc acttcaggtg aatcaatcct atgtcggcaa gacactcgat
    38221 gtgcttgtgg aagggttcga caacggcatt tccattggac ggtcttatcg cgatgcgccc
    38281 gaaatcgacg gaatggtttt cattgaaggg aaggcaaatg tgggtgagat cgtaccggtc
    38341 cgcatcagcg gcgcaatggc ctacgacctg acgggcgtgc tggcgcaatc acaacagcag
    38401 gtgatcaaac tataacctgt tgtgtggagc agttcatgcg caagataaat cttgcggtac
    38461 aaaagcggct gatactgaaa agtatcagcc gcttttgatt tttaggatgc tcttgcaaat
    38521 gcagttgcgc cgaggatcag cgccgagagg atcaggttcg ttctcagcag gatgacctcg
    38581 cgcctttgca ggctggcaag ctcttccgga ctggcttttt cctttttcat cagtgtgcga
    38641 tgaatggccg ggataacctc ccaggtttgg acggcgctca caatgcccat gatgaggctc
    38701 aagccatgtt taatgagaat ggacagcgac cattgtgtgc tggtgtcaaa gaagccatcg
    38761 tagtgcgaat tggcgctcaa ttgaaacagc ccggttgcaa ccagtaagcc catgctgaac
    38821 catgccaatg gctcaagctt tttttgaagg gcggcgataa agctgagttg gtcggccagt
    38881 ttcaaggtcc gctgtgatgc gggcaggacg agcaggttga tggcggcgag gcttccgatc
    38941 cacgtgactg tggcgagcat gtgaagccag tagatgatcg ccattaccca tgaggcgggt
    39001 gtggtcatgg cttagggtgc gggtgtgatc ggtgtgggtt ccagtgtggg cggaacttcc
    39061 gtcggcgggt agcactgtat ccctgcctct tggaagtggt tttgggaacg gttatccagg
    39121 gtgccaaggc ctgctgcgat ctggtattgc tcgtaggcgg cgcagtagga ttgttggtcg
    39181 aagagttcgt cgccgtagcg catcgctgag atgaagagac gctgtgagcc ggtcatggtt
    39241 ccatcccaca tgccgggcca acctgctccc acctggctga agtagttgta ggcttgtttc
    39301 caatccaatt cccagaaact tgcgccggtg atgtacatgc gggcgccttc tcgcaattgg
    39361 ttggcggtgt tatccagtgg accaaaacgc tcggccagag tcaggtgata tatgccgcct
    39421 tcgagattgc cttgttgatt aatgagggca atgccatagt tgcgcagggt gaaatagtac
    39481 atgccatcca cctgggcggc tttgtaggtg gggtcgagtt tgcggacaat atcgagggct
    39541 tcgagggcgg caggccaatc ttgcgcattg accagttgct gggcacgttg ataggcgctt
    39601 tcgacgccgc tgaaatcagg ggtggaggtg ggcgtgggca ccaaagtggg ggtggggatg
    39661 acgctcatca ccatcacttc ggtgagcttt tgctgggcgc cggggaagcc ggggtcgttg
    39721 gcgatgatga atttcaggcg ctgcatcgcg gtttcgtaat tgcccagctg gatgtcggtc
    39781 aatgcgaagg cgtattgctc agtgagctgc ttcgagatga cggaatcttc cgcattttgg
    39841 cggatgccaa taccggagga gtatccgccg tatgcaccca gcccaaccag caggagcaga
    39901 ccgaagagac cggtcaggat ggagcgccag cgggaaggtt tcttgggcgg tttgatgggc
    39961 tgggtatcat ccacctgagg ggctgattca gcctgggcgg tttcagtttc cacagtttcg
    40021 ggctgtgaat tttcaggttg aggtgtgaca ggttgtgtgt tttcagtttg ttcggacatg
    40081 agtgtaatta tacttccctt tgattttacg gttttcccgt ttttaacgaa aaaaacagga
    40141 accttgccgt gtcgaaataa gttttataaa aagatgctcc tggcggtgtc cccgccgccg
    40201 aggatggcga ctggagcagc cgcagatttg ggataacaat ccgcgaaaat cagcgaaatt
    40261 tgcggcaaaa gccctattga gctgtggcta ttccgccaga gacgagatag ccacaagttc
    40321 ttaattcagg ttcaggcggg gtgcgacctg ctccggtaga tttacaactt cagcagtatc
    40381 tttgcatctg accagacgat cgccagcgcg atcagtccgc caaccaccat tccgatcagg
    40441 gcggtgatga tggcgccgcc gggcaggtaa agtgccacgg cataggtcac caccccgccg
    40501 atgaccgccg caatcaaccc tctgacaacc gacccgccga catgaatcgg ttcatgggta
    40561 cgattcgaca gccatttgaa caggatgatg gcttccacca aaagtgcgat ttcagcaaaa
    40621 gcgatgatcg gggcgccgat ttccttgaaa aaggtcaggc caatgaaccc gattccaagg
    40681 aacatgacaa ggcgcaagcc cactgcataa agcggaaaca aaggctcctt gcgggcataa
    40741 aacgagcgcg ccgcgatttc gtggatggtg aatccggtca gggtcatcag ataggcgcgg
    40801 gtggtccatg tgacgagggt ggaggtggct tcgtcaaagc caaagacacc gcgtacgagc
    40861 gggtgaatgc ctgctgccat caccgctgca atggggatgg taagtgcgat caacacccgc
    40921 agggcgcgtt caatggtgga gcggaattcc acccagtcgt ggcgtgccgc aagctctgag
    40981 agtgcgggca gcatggctgt agcaatggct gtccccagga tggtttcagg gacttgcatg
    41041 atcatccagc cataggtgag cgatgtcaca gcgccgactt gatcaagacg cgaggccaga
    41101 ttatcgcggg caagaacgat gagttgaatc ccgcccatgg tcagcagacg gggggccatc
    41161 aatttcaagg cttcgatcaa acctgtgtgg cgaatatcca atgagggggt ccagcggaat
    41221 ccgaatttga acagcattgg cacttgaatc aacaagtgca tgagcgcgcc gatgatgacg
    41281 ccatatacca gaccttgaat gccgtaacgc ggcacaagga agatcgcacc aatgatctgc
    41341 ccaatgttgt acatgctggg ggccatcgca ggaaagataa aatgctggtt ggcttgcagg
    41401 gaagccatca ccaatccgct gatggaaaag atgatcgtgc cgatcaggtt caggcgcatg
    41461 agttcagcca agagcttgcg ctgttcctcg ccgaagcccg gggcaatacc gaggttggca
    41521 tccacgatgg gttgggcaaa gatggcgatg acgatggcaa aggaacccgt caccaaaaaa
    41581 gccacattgg ccacgcgtga aaacaggtcc catgcggcgg cgcggtcttt ggtggtgaga
    41641 tactccgtca gcagcggaat gaacgccatt gccagcgctc cacctgagat gagagcgaaa
    41701 agtacatcgg gcaaattgtt tgcggcgttg aaagtatcca gcatgtgaac cgaatcggaa
    41761 tactgccgcg agataatacc cgtacgcacg aaggcgaggg ctttatccac cagaaagaaa
    41821 aatgcaatga tgagcgagtt acgggtaagg cgtgaaagtt tattcatttt taatgagccg
    41881 cgtctggcgc gatctccacg ccggggagtt tatctgcgat cagatggatc ttatggatca
    41941 acaccggcag gcattgcggg caaatgtatt tggtttcatt tttaaaagtt aaatgcagca
    42001 acggcacttg ttcatcggtg cggttgcaat tcaggcagtt tttttcgttt tgttcggtca
    42061 tggttctcct ttgttttaac gttttaacgt tcgaacgttt gaacgttaaa acgtttgagt
    42121 gtaaaaaatt aaaccccttt aaccgtgccg ccatccacaa gcagcatgac accggtgatg
    42181 aatgatgcgg caggggagac gaggaacaca gcggcattgg cgaactcttc aggttgaccc
    42241 atcctcccaa acggactctc cgccgcctgc cgttccgttt cttctccgac cgtggaactg
    42301 ttcttttttg cccggtcagt catcaactct gtgactcgtt ccgtctccgt ccaacctggc
    42361 aaaatcgagt tgacgcggat attctccctg ccaagctcca gcgcgagtga tttggtcaac
    42421 cccaccgttg ccgcgcgaac actgttcgag atgagcaggt tgggaattgg ctgcctgacc
    42481 gaatacgaag tgaccgtcag cacactggcg gattttgatt ttcgcaaatg aggcagcgcc
    42541 gatttgatca agcggacgtg cgccattaaa cacaggtcaa tgcctttttg ccatgcggct
    42601 tcgtcgaggg catcaatgga acctgcggtg gggccgcccg tgttggtgat gagaatatcc
    42661 aatccgccaa aggcttcaac agtttgggcg accaacattt caggtacgtg aggcaggctg
    42721 acatcaccgc ccaacccgat cacttccgtc ccagtttcct tgctgagttt ctccgccact
    42781 cctttgatct tcgcctcgtc ccgcccattg atggcgaccc tgcagccctc ctgtgccaaa
    42841 gttcgcgcgg cggcatatcc caacccacgc gaggatgcgg cgacgagggc gattttgttt
    42901 ttgagttgta gatccatgtt gtctcctgtg gtgtaacgtt gtaacgttca aacgtttgaa
    42961 cgttacaacg tttataagtt ctctattttg ctgtcccaaa catctccgtc ggtccagttt
    43021 gcttccaccc attttgccac actttgcaga gactgtcgca agaccaccgc gtcggaattg
    43081 agcatcgcgc aggcgatgat ggcttcatcc catgtatcct gcaaatccat ctcagcgaca
    43141 gacacgttga attcacggtg caaacgagag agcagcggct tgattcgtcc gcgcttttct
    43201 ttgagggagg tacataaggg cagtcgaagg tggatggtga ggatagcgag catggtttga
    43261 ttttcgattt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    43321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aagggcagtc
    43381 gaaggtggat ggtgaggata gcgagcatgg tttgattttc gatttgagat tttggattta
    43441 cgatgtatta ccagccaatt atcattgaaa gaataaggac aatacatcct gtccaacaga
    43501 aaaataaaat taaatagccg atatactgtg acccggcgcc atggattaaa tagccgtact
    43561 ttgagctgat tcttacgttt cttttgacca tttgatagcc gtccaggctt ataaacaata
    43621 aaaacaaaat agaaattgaa ttgacggtgg ataagttttc gctttgtttt ccaaagaggg
    43681 gaacaatgaa gaaaatgttg aaaaaacacc ataaaaatcc gatcgtggta tggcgtctgg
    43741 ttggatgtaa gtaggctctt aagtccttga ataatggact tgatggccgt ttgatttgct
    43801 gaccaagcga gtcctttcta aatttgtgtt caatactcgg ggctattgca acgaggatca
    43861 atgtggcggc cattgctcca atgaatattc cgattaccag attttgatgt aggaatttca
    43921 ctgaaaaaag cccgcccaga ccaatacaaa caaaggctat gccaatatag atgaatgtct
    43981 tgttgctttt tatgaaattt tcccagtttt tcatttacta gacctgtctg ttttattaga
    44041 ctacaatccc gcacatggat attgaaacca aatacaatca agcgctcgat tatctttatt
    44101 cctttgtgga ttatagcctg aaacattcct ccgaattggc gaaggctgat ttcaaccttg
    44161 accgcatgtt tgcgttgatg gagtctttgg ggaatccgca aaagaaatat cccatcatcc
    44221 atgtggcagg gacgaaggga aaaggctcca cttcggcgct ttgcgcttcg gcattgagag
    44281 cggctgggta caaggtcggc ttgtacacgt caccgcattt gttggattac accgagcgga
    44341 ttcaggtgaa cggcgcgccg atctcgcatg agcaactggt tgcgttggtg gatgagatcc
    44401 gcccccacgt gtcaaaaatt gaaaaattga ccacctttga gatcaccacc gcattagcct
    44461 tcatggcttt tgcgaaatat ggcatggatg cggcagtgtt tgaagtaggg ttgggcggac
    44521 gcttggatgc caccaatatt gtgacgccga atgtttcgat tatcacttca ttatcgtacg
    44581 atcacatggc tgtattggga aataccctga cgctgatcgc gggggagaag gctggcatca
    44641 tcaaacctgg catccccgtg gtttcctcgc ctcaaaagga cgaggctttc caagtcttgg
    44701 agcgtgtagc caaagaaaag agtgctgggc taaccctcgt tggcagagat gtaacattcg
    44761 agttggtcga gtcatccttg gatgggcaga agttcaacct gcaaccttca actttcaacc
    44821 atcatccttt atccttcgtt atccccttgc ttggcgctca tcagatcgag aacgcagcca
    44881 cggcttatgc cgcactcaag atcagcggaa ttcccattac agatgagcag atacaaaaag
    44941 gcttttctca agtacaatgg cgcgcccgct tcgaagtggc gcggcgtgaa ccgcctgtga
    45001 tcttcgattc agcgcacaac caggattcat ttcaaaagtt gggcgaaacg cttgaaacgt
    45061 acttccccgg taagaaggtg atcctgatct tcggcgcatc ggaagataaa agcattctcg
    45121 atatgtttac cgccttaaag ccgaagattc aaaagatcat tgtcacgcgt gccgaccatc
    45181 cgcgggcgct tgaggtggag cgaattcaac agttggctgg gcaggctggg gcggagtcgg
    45241 aagcggtcac tcctgttaag tccgcgttga tgcgggcgct ggaattgtca tcaaatgatg
    45301 gtagcattgt cttatccgct ggttcgatgt tcgtcaccgc ggaagtcatg tctgcatgga
    45361 aaacgagaaa cgaatgaatt atcaaagcga ctggaacgaa aacgaagata actttgacct
    45421 cacgctctca cagcggaccg aggaactgta tcgtgtgcca aatatggagc ccaacgccga
    45481 cggtttgatc gaggcggtgg tgctgccgtt gcgcgacatg gtcatttttc cgcgcatggt
    45541 ttcgccgatc tttgtggggc gcgaggcttc gctgttggcg gtgcgcgagg cgcaagccaa
    45601 agagcagact gtgatcgggt tgacccaaaa agaccctgac ttgaatgacc ccaacgccga
    45661 agactttttg cccgtcggtg tggaaatggc tgtggggcgg atgctgcaaa tgcccgatgg
    45721 ttcctactct gcgctggtgc agggacgcaa gcgcgttgag atcgttgaat ttctgcgtgt
    45781 gaagccctat atcaaggtgc gtgcgcgggt gatcaatgag ccaacttccg ccgacaggca
    45841 gacacaggcg ttgatgcgtt cggtgttgag tttgttcgag cgttgtgtgc agttggatcg
    45901 ctcgatccct gaagaagcgc atctgttcgc gatgaatatt gcggagcctg gctggttgtg
    45961 cgatatgatc gccacttcgc tttcattgac ctacgaagcg aaactgcggc tcctgctggt
    46021 gcttgatccg cgtgcgcggc ttggattact gaacaatttg ctggcacagg aagtggatgt
    46081 gctcgaattg gaagatgaga tccattcgcg tgtgcaaagc gaagtggata aaagccagcg
    46141 cgagttttat ctgcgtgagc agatgaagca gatccaaaac gagttgggcg aaggcgatgt
    46201 gttttcgcgt gatgcttcag atttgaagaa aaagctggat gccgccaacc tgcctgatga
    46261 agtgcgcaaa gtagccatga aggaattgga gcggctcagt cagatgccgc cgatggctcc
    46321 cgaagtgggg atgatccgca cgtatattga ctggatcgtt gacctgccgt ggagcaattc
    46381 ctcgccagat aatcttgatg taaaaaatgc cgcgaagatt ttagagcgcg atcactacgg
    46441 cttgaagaag gccaaggacc gcattttgga atacatcgcc gtgcgttcgc tgaagccgaa
    46501 aaaggaacgt cagccgatct tgtgttttgt tgggcctccc ggtgtgggca agacctcgct
    46561 tggccgttcc attgcagatg cgcttggacg caagtttgtg cgtgtttcac tcggcggtgt
    46621 gcgtgacgaa gccgagatcc gtggacatcg ccgtacatac atcggcgcat tgcccgggcg
    46681 tatcatccaa accatgaaac gggctggtac gaccaatccg cttttcatgc tcgatgaaat
    46741 tgacaagctg tattcagatt ttcgcggcga ccccgcctcg gccatgcttg aggtactcga
    46801 ccccgaacaa aactacgctt ttagtgacca ctaccttgaa ctcccctttg acctttcgaa
    46861 ggtcatgttc gtgaccacgg ccaactcgct gggcagcatt ccctcgcccc tgttggaccg
    46921 catggagatc atcgaattcc ccggctacat tgaagaggaa aaggttgaaa ttgccaaccg
    46981 ttacctgatt ccgcgccaat tggaagaaag cggcgtggat aaccttggtc tggtctttga
    47041 acctgccgca cttcagcgca tcatccgtga atacacctac gaagccggtg tgcgcagtct
    47101 ggagcgcgag atcgggcgtg tgtgccgcaa agtagcccgc atgaaagcgg aagggaagaa
    47161 atatccgctt gcgatcgcgc ctgaacttat tgaaaaatta cttggcccgc aacaggtctt
    47221 tccaccggaa gcggaacgct ccgatgaagt gggcgttgcc accagccttg catggacgga
    47281 gaccggcggc gagatcatgt ctgtggaagt ggccgtgctc gagggcaagg gcgggatgca
    47341 gatgacaggt cagatggggg aggtgatgca ggaatctgca caggctgcgt tgacctacat
    47401 caaatcgcgc gcttccaaat tgaagatcga gctggaagcc ttcgaacggc tggatattca
    47461 cgtccacatg cctgaaggcg gaatcccgaa ggatggcccc tcggcaggta tcacaatggc
    47521 caccgccatc atctccgcgc tgacggggcg tcccatcttc catcatgtgg gcatgaccgg
    47581 cgagatcacc ctgcgcggac gcgtgctccc gattggcggc gtgcgtgaaa aggtccttgc
    47641 cgcacaccgc gccgggctga agaccgtgct gctgccggaa aagaacatga aagatctggt
    47701 ggacctgccc aagaccgcca aatcggaatt gaagatcatc ccgatcaaac acatggatga
    47761 tgtgctggct ctggccctct ctgaaaaagt gaccgttgaa cctccgcgcc cgaagaagca
    47821 agcgcgtgat gacagccagg aagattgaaa aagatatcca cagatgaacg ccgatttttt
    47881 gaatcgcctt tgaatccctg tttatctgtg gctaaataat ttctcaaagc ggcgcggtga
    47941 taaaattacc gtaccgcttt ttgttatcac ccctgaaaac ggaaaaacaa atgtcgaaac
    48001 tgcaaaacaa agttgtcctc attaccggcg cctcctcggg gtttggcgaa gatgccgcca
    48061 tgctctttgc gcaggagggc tgcaaggttg tgctggccgc gcgccgcatg gaccgtttgc
    48121 agaatctcgc cgagcggatt caatccgcag gtggtgaagc gattgcaatt ccagtggata
    48181 tcgtcaatgt ggccgatgtg gataacatgg tgcaaaccac actcgacctt tatggtcata
    48241 ttgacatcct cttcaacaat gcgggcatcg gccgtgtgga atggttcgaa aatcacaccc
    48301 ttgaacgcga cctcgataca ttggtgcatg tcaacctcac cagccttatg caagtcaccc
    48361 gttcagtcct tccccatatg ctggcgcggg gggaggggca catcatcaat atgatctcag
    48421 tggcggggct gcttgccaca ccgctcatca ccagttatgc cgccagcaaa cacggtgtgc
    48481 gcggtttcac cgatgccctg cgccgcgaag ttgcgccgct cggcatcaag gtcagcggca
    48541 tttaccccgg ccccgcctcc accgaattcg gtcagcatgt cggcaaaaac gaggcttatc
    48601 gctctgtccg ctcgcggctc aaaattcata tgagctcaag ctatgtggcc cgccgcgtgg
    48661 tggatgtggc taaacgtccg cgccgtagtt tgatcattcc gtggtggttc cgcatcctca
    48721 ccacgattga agcggttttc cccgcccttg tagattggat ctcatactca ttcacgaaaa
    48781 agaaacataa attggattag gagacttaaa tgacgaccgc ccgactcaaa agccttatcg
    48841 attccctccc aacctcgaac ctggatgctg ttattctgaa tccgggtcca accctcacac
    48901 atctgaccgg tgtccacttt catttgatgg aacggcctgt tgtgctattc gttgcgcccg
    48961 gtcaagaccc ggcgctcgtg ctgcccgaac ttgaactacc caaattggga ttattcccct
    49021 acaaagtgca ggcgtttgcc tacggtgaaa atccgtcaga gtgggacgat gcctttcgaa
    49081 aggcggcgca ggccctgggt ctggatggga agcgcattgg agtcgaaccg cgtcaattgc
    49141 gcttgctcga ttttcgtcat gtcaaagcgg gggcgcctga agcggaatac ccggatgcga
    49201 gcgaagtact ttcccgtttg cgcctgaaaa aagataaagc cgaagtggat gcgatgcgtc
    49261 gtgctgtgaa gatcgcgcag gatgccctcg aagccaccat ccctttgatc aagatcggaa
    49321 tgaccgaacg tgagttgtct tccgagttgg tcatccaatt gttcaagcaa ggctccgacc
    49381 ctgaattgcc ctttgcgccg atcgtctctg gcggacccaa tgccgcgaat ccgcacgctt
    49441 caccgacgga aagaaaatta caggcaggcg acctgcttgt ggtggattgg ggcgcagcct
    49501 tcgatggcta catctcagac ctgacccgca ccttcgcagt gggtgaggtg gatgatgaat
    49561 gcaacaagat ccataaaatt gttcaagagg ccaatgccgc aggccgcgct gctggcggcc
    49621 ctggtgtgtc ttgcgccaat gtggataagg ccacccgtga tgtcattgaa gcatcgggct
    49681 atggaaaata ttttacccac cgcaccggtc atggcattgg catggaaggc catgaagaac
    49741 cctacatgcg aggcgacaat atgcaattgt tggaacccgg catggcctac accgttgagc
    49801 ctggcattta tctgacggac cgcaatggcg tccgtattga agataatgtt gtcatcacag
    49861 aaaacggcgc agagagttta agcgatatgc cgcgcgagat tcgcgtggtt ggatagacag
    49921 ggttgcaaga tcctgtgtat gtgatttgcc gtgccacttc cactgatgcc gaccgcctga
    49981 cagagatcac catcgccgcc aaacggcatt ggaattaccc ggaaaagtgg atccaattct
    50041 ggcttccgca attgaccctg accgcagagt acatttcaaa acacgaagtt tggatggtca
    50101 tggcaaagga tgccgctatt ggttattatt cgcttgagta ttctgacggc gagctttggc
    50161 tgggaaatct ctgggtattg cctgaacaca tggggcaggg gatcggccgt ctgctttttg
    50221 aacatgccct gaaccgatgc cgcgggcttg gttgctccac cttgaagatc gaagcagatc
    50281 ccaatgccca aagcttttac gaaagaatgg gagcatgcaa aatagacgag cattgcagcg
    50341 aagtggataa tcagccgcgt atattacctg tcatggaaat tgttgtacca gcttgatgac
    50401 actcaagatc atgacgcacc tcgaaatctt aatttcgagg tgcgtttttg tttgcgaccg
    50461 ggatttgccc ttgaaatttc accaaaacct gctatgattg tttgtgattg attgatgagc
    50521 gattatgagc gaatttaccc ccacccctga acggcaaaaa cagatccttt ccctgctcgg
    50581 caaacaggga cggttaagcg tcgctgagat cgtggagcaa ttctcgatca gcgaagccac
    50641 tgcaaggcgc gacctggaaa cgcttgcatc tcaaggcaaa gcccaacgcg tgcatggcgg
    50701 cgtgatcgcc ttggagcagg cacctccaga gcttcccatt cttgaacggg aaggcgaaca
    50761 aacggacgaa aaagccagca ttgggcgcgc cgccgcggaa ttgatcgccg atggcgaaac
    50821 ggttttcctc ggctcaggga caacggtact cgaagccgca aaacatctgc gagatcgaaa
    50881 aaatctcacg gtcatcacca actcgttgcc tgtgttgaac gtccttgcgg gtatcaaaga
    50941 tattaccgtc gtttcattgg gtggacagtt gcgcgagagc gaactttcct tcatcggtca
    51001 cattacggag caggcactcg ccgaactgcg cgtggacaag gtcattatgg gcactcgcgg
    51061 aatcagtttg gaacatggtc ttacgaatga ttatttgcct gaaactctca cagaccgcgc
    51121 catcctgaag atcgggcgga cggtcatcat tgccgcagac cacacgaaaa ttaatcgggt
    51181 ggctaccgct ttgctagctc cattgaagtc catgcaggtt tttgtgacgg actcacatgc
    51241 agagaagaaa tttttgcagt cgttgaaaaa gctcgagatc gaaatagtca tcgcgtagat
    51301 caacttcatc atttaggaga accatgctcc tcaaaaattt tcgtccacag tccaaactag
    51361 tcaccaaaac cacattggtg aacaaaccca aattccctgt gatcgacgcg cacgatcatc
    51421 tgggtgaacc cttcggtggc ggctgggata aaaagccgtt gagcgaatta ctcgaccttc
    51481 ttgatgaagc aggcgtgact cactatgttg acctcgatgg cggctggggc gaagacattc
    51541 tcgatgccca tctcaaacat ttcaaagaag gcgcacctga acgatttcaa gtcttcggcg
    51601 gcgtggattg gaacaaatgg caggagttgg ggaatcaatt cccagagtgg gcagcgaagc
    51661 gtttgatcgc acagaaagag cgcggcgcac aaggactcaa aatttggaag ccgcttggct
    51721 tgcatgtcaa agaccatcaa ggcaaattgg tcgatgtaga cgatatacgc ctctccccca
    51781 tttgggaaac cgcgggggaa ctcggctttc ctgtgctcat tcatgtcgct gaccccgttg
    51841 cattctttga ccccatcgat gaaaccaatg aacgctggga agagatcggc gaacatcccg
    51901 attgggcatt taccagcccg cccttcccat cattcatgca catcatgaac tgtttcaaga
    51961 acttggtcac tcgccactcg tccactactt ttatcggggc gcatgtgggt tgctatggtg
    52021 aaaaccttgg ctgggtcggg cagatgctcg acgattgccc aaactactat attgacatct
    52081 ccgctcgctt cggcgagatc ggacgccagc cttacacctc ccgccgtttc tttattcaat
    52141 atcaagaccg tattctttat ggctctgata tgagccccga ccttgatgcc tatcgtctca
    52201 gctaccgcct gctcgaaacg gacgacgaat atttcaatta caacaccagc gaagtccctg
    52261 ggcagggacg cttctatgcc tatggcttgt atctacctga tgatgtcttg aaaaaagtct
    52321 atcgcgagaa tgccatgaag gttttgaaat tcacgtaaac atttgtggtc tcgatacagg
    52381 cttcgcaacg aacgctcagc cctactcgac caccaaacta tgagactacg agactatgca
    52441 accaaccgac taccacacct acaacgaaat caaatcacag actcaagcct gggcacaggc
    52501 att
//
LOCUS       DAOQLC010000010        49796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_175_length_49796_cov_27.179148, whole genome shotgun sequence.
ACCESSION   DAOQLC010000010 DAOQLC010000000
VERSION     DAOQLC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 49796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175_length_49796_cov_27.179148"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..407)
                     /locus_tag="PL000_03215"
     CDS             complement(87..407)
                     /locus_tag="PL000_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87933.1"
                     /translation="MTINSFRVTPWESASPPTEELLFAIMAGQGLNPYSWSNGPHDVY
                     SAHKHGFDKVIYVVRGSITFGLPLLKKQITLHTGDRLDLPAGTVHDAQVGPEGVVCLE
                     GHLD"
     gene            complement(404..778)
                     /locus_tag="PL000_03220"
     CDS             complement(404..778)
                     /locus_tag="PL000_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87934.1"
                     /translation="MSILGVDHVQIAIPAGGDAEREARSFFIGLLGFREVPKPPELAQ
                     RGGLWLEAGSVKLHLGVESDFHPARKAHPAFIVRGLDELLAKIQRAKFETDTTQPPLE
                     GYQRAHVFDPFGNRIELMEKTG"
     gene            complement(900..1274)
                     /locus_tag="PL000_03225"
     CDS             complement(900..1274)
                     /locus_tag="PL000_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC87935.1"
                     /translation="MTRQRNIFIGLSVALLTVLACNAILPQNEPDAPISTQAPIFTLE
                     PRPTANSRLPESEAAVPRVTAEEAKAALDSGEAIIVDVRGPEAYAEAHIAGAVLVPLG
                     NFEGDIANVSLDKNKWIITYCT"
     gene            complement(1363..3165)
                     /locus_tag="PL000_03230"
     CDS             complement(1363..3165)
                     /locus_tag="PL000_03230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNC87936.1"
                     /translation="MTLSAPHWDLTNVYPSLESKEFKAAVDDYKKQVASFGKFFKNKL
                     GKVTPKTKAREIAPLVGEAIDRINSIQTLSSTIFPYIYSFVTTNSRDKVAMKTLSEME
                     QASLPMSQLYTQFTAWVGANAKKLGEIIKLDPSAKAHAFMLKESAQQSKYLMSEIEEQ
                     LAAEMSLSGGNAFGKLQGAVVSQMSVEFELNGKVEKLPMPALINLRSHPDESVRRRGY
                     EAENQAWEGVKETLAACLNGVKGEVIVLNKRRGRKDALHSAIEAARLDRKTLEAMLEA
                     MKDSFPMFRKYFKAKAKKIGKEQLAWWDIFAPLGKTDKVYSYEEARDFILENFEKFSP
                     DLSTFAKRAFDNNWIDAEQREGKRGGAFCTEVQALKESRILCNFDGSFDQISTLAHEL
                     GHAFHNECAYQADKTVLQQLTPMTLAETASTMCETIVTEAVLSTTKDPQEELAVLEAQ
                     IQGASQVVVDIYSRFLFEKEVFERREKSELSADDFNDIMERAQKATYGDGIDEKYLQK
                     FMWTWKPHYYSSGLSFYNFPYAFGLLFATGLYAIYQQRGKDFVEDYKNLLASTGEESA
                     AKLAKRFGIDITTRKFWDDSLAIIGKRVDRFCEL"
     gene            3277..3750
                     /locus_tag="PL000_03235"
     CDS             3277..3750
                     /locus_tag="PL000_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87937.1"
                     /translation="MKQDRFLTGILAGIGVLILLALVLFFVRQDDTKEYRVDASPEAT
                     VYNYVLAITNDDYQKAYSYLAEKEHKPSYEQFRQSFFNGNVSPDNVGVDVGTAEISED
                     DALVNLSLVYSPNDPFFGGYSSTDRAQLIHQNGEWKLTYMPYNFWSYDWYQEQFK"
     gene            3774..5699
                     /locus_tag="PL000_03240"
     CDS             3774..5699
                     /locus_tag="PL000_03240"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HNC87938.1"
                     /translation="MKTVRRLYFYAVALISFEVVLWGLVGLIRSIVNPNLITDNAQAL
                     ATALSLILVGIPFFLIHWLWAQRVSARDEEEKTATVRAVFLYGALFATLLPAVQNLLA
                     LINRTFIGAARISYERALVGGTQSWPDNLIAIILNLLMAAYFWSILRAEWPTLPDVEN
                     FKDIRRVYRYLWMLYGLLMVVFGTQQILQFILQIPTDLIGTSGRETFVNATALLVVGT
                     PIWFFAWRFIQDALPDPDESGSVLRLVILYLLSIGGTITVLAAGGNILYDLLSRLFGE
                     NISNTDLLQNISDPLSLVIPFGVLWGYFGGQLNRQFDAETNIPRRSGMKRIYFYILSI
                     IGLGATVTGVLSLFVVIVNQLSGKDVDVIAADSVLKQLASAISVIVVGLPLWLKAWNP
                     MQAEALADGESGGHARRSITRKSYLYLVLFASVLGTMAPAGMIIFLLIQAALKGGVEN
                     DFLNSILNAFQALIVFAVVLTYHLLALRKDGAAQADVLEEKQSEFRVVVFDHDGKFGE
                     ALKAVFTRHAPKLPVTVLMVGQPIPVDLKANAVVLSGSLAVNLPDHVEAWFRSFNGSR
                     LIVNDDAAGIYWMNDFGQAALSAQALAEGQEIRPRSAVKSTPIWTYVAYVFAALFACQ
                     LIFILLSLGISLVVGGF"
     gene            5770..6903
                     /gene="dnaN"
                     /locus_tag="PL000_03245"
     CDS             5770..6903
                     /gene="dnaN"
                     /locus_tag="PL000_03245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC87939.1"
                     /translation="MKVTVLQENLARGLSTVSRAVSPRSTLPVLSNILIASDEGRLRL
                     SATNLELGITCWIAARIDEEGSTTVPARTFADLVNTLPGDQVQLNLDVKTQNLHVKSG
                     SSNNDIKCIDAQEFPPLPVPEMDGAVQLNVADFKEMIQQVVFAASTDEARPVLMGVLM
                     HVEKDKLTMAAADGFRLSVRKAVLSNPVPQPLNLIIPSRALSELARVASDPEEPIYMV
                     APKNRGQVLFRVKDVEVVSQLIDGTFPDYQQIIPRSYKSRTLVSTASLLKACKQAEIF
                     AREGSNVARFDIKQSNGEMQPSEVEISATSEETGKNETIVEATVDGGSVLIAFNVKFL
                     REALEVIKTPNVALETSAANAPGVIKPVGDDNFLHVIMPMHLG"
     gene            complement(6984..7775)
                     /locus_tag="PL000_03250"
     CDS             complement(6984..7775)
                     /locus_tag="PL000_03250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HNC87940.1"
                     /translation="MILLPIVAGFLIARNFNFGFKDFRKLFTTGALTFIAAQMLHIPV
                     VYGLTALFSSGILPAPDKSITLIFNAIVLGLLAGIFEETARYILFRTIRKSMDTWQTS
                     IVIGLGHGGIEAILLGIISISTMIQMIAMRNPINLSALNLPADQLDIVQQQVAAYWAQ
                     PDAMPLFGFMERISALSLHIGLSILVLYSIISNQKKWFWIALLWHAFIDALAVYVFPK
                     ITAGENVITGVFLFELLILILGGGVLIYAIRFRTSFPAKNEAPTN"
     gene            complement(7914..8564)
                     /locus_tag="PL000_03255"
     CDS             complement(7914..8564)
                     /locus_tag="PL000_03255"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNC87941.1"
                     /translation="MNTKTTSIIALGMIVIAIMAGVFLWNQLPEQMASHWNANDEVDG
                     TMPRFWGVFLMPIVTLGMFALFIFIPNMDPLKANIVEFRETFNLFIVFFVMFMLYVHT
                     LTLIWSLGYNNFKMSTTLLPIIGLMFIFIGYMLRKARRNFFIGIRTPWTLSSDTVWSK
                     THQLGSILFMLSGVFAMIGGFFGGMLAFWLMFVPLIGSTLFLVVYSYVLYRNEIKA"
     gene            complement(8600..9541)
                     /locus_tag="PL000_03260"
     CDS             complement(8600..9541)
                     /locus_tag="PL000_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNC87942.1"
                     /translation="MNTSLFKSLLEKNKQTKEDQHISPIAITIFTVFLLVAVFGAVIG
                     DGRFSDILIGGWVLGWTMIGAYFLFALRVASQWEKAVVLRLGKFHGLKGPGLFWLIPI
                     IDNATMWIDHRVMVTSFSAEKTLTKDTVPVDVDAVLFWVVWDAEKAALEVKDYQVAIS
                     WAAQTALREIIGQMSLADILVGRAKMDEELQKIIDERTTPWGVTVQSVEIRDVVIPQV
                     LEDAMSRQAQAERERQARVILGESEQQIAISFAEASQAYINNPTALHLRAMNMLFEGL
                     KEKGALVIVPSSAVDSMNLGGLSGMVSLAQNNQVKEK"
     gene            complement(9538..9966)
                     /locus_tag="PL000_03265"
     CDS             complement(9538..9966)
                     /locus_tag="PL000_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC87943.1"
                     /translation="MTEKKLTLRLDFHSGLPIYTQIVNQIQSQLANGILMPGDQLPTV
                     RALAQELRVNFNTVARAYRILDEARVISTQQGRGTYITEIPPPKVSEKLRSESLATLT
                     QRFISEAIRLGFSEREISQMVRDNLKSMKETLEQQKENVK"
     gene            10304..11191
                     /gene="pdxS"
                     /locus_tag="PL000_03270"
     CDS             10304..11191
                     /gene="pdxS"
                     /locus_tag="PL000_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNC87944.1"
                     /translation="MESKTGTWTEKKGLAQMLKGGVIMDVVNAEQAKIAEDAGACAVM
                     ALERVPADIRADGGVARMSDPDMILKIMDAVSIPVMAKCRIGHFVEAQILESIGIDYI
                     DESEVLTPADEEHHILKHNFKVPFVCGCRNIGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARSVIGSIKQLQVMGDEELMTFAKNNGAPYELVKEVKELGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKSVTHYQDASILAEVSKNLGEAM
                     VGRNAAQMPESEKLAVRGW"
     gene            11238..11816
                     /gene="pdxT"
                     /locus_tag="PL000_03275"
     CDS             11238..11816
                     /gene="pdxT"
                     /locus_tag="PL000_03275"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HNC87945.1"
                     /translation="MKIGVLALQGDFAEHISMLKKIGVDTAEVRLPKHLDGLDGLIMP
                     GGESTTFGKLAVAYGLIDPIRQFGKSHAVWGTCAGAIFLSKDIGRDQPLLGLMDIKVQ
                     RNAFGRQVDSFETDLEIDELMKATGTEHPYHAVFIRAPIIESVHGNARVLSALEDGRI
                     VAAQEGHLLATSFHPELTDDTRFHEYFISLAS"
     gene            11861..12778
                     /locus_tag="PL000_03280"
     CDS             11861..12778
                     /locus_tag="PL000_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87946.1"
                     /translation="MSIRPLDLLDLPVITRYRNNVLTLDSARALTRGHPLGAMGLLAY
                     INPARHLYAAIANGGDTALLGGVIHTRGETFAKLLYLAPSSNLGDPTLPALIEHLAAQ
                     AGDWKAFHVLAEVDESSDVFPALRTAGFSVYAWQRLWDVSLLEKTESDISLRWRRTQS
                     LDLPAVQSLHYQIVPPLMHPVEPAPKRVLGFVCQNLKCYASVTAGMYGIVLTPLIHPE
                     EQNVGEKLLALVAGLPDRRGRKVYLNVRSYEAWLEPVLADLGAQASSRQAVMVKHLAR
                     LIKEGQSVRTVPTNVGVPASRISRMDFDE"
     gene            12771..13091
                     /locus_tag="PL000_03285"
     CDS             12771..13091
                     /locus_tag="PL000_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87947.1"
                     /translation="MSNWATYDGGRSIGKTSPAGDVILYDEEHHLGARITLRRGKNFI
                     SVSCNFYGWMDHTRFFNALSEAEREYRAMKTAIAAMMEPLKAAASNKLKGWEAISEFV
                     RRFP"
     gene            13093..13764
                     /locus_tag="PL000_03290"
     CDS             13093..13764
                     /locus_tag="PL000_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC87948.1"
                     /translation="MLKALIFDFDGLILDTETPEVLVWQSIYRDHGFELPVDEWEKTI
                     GGYGLSNFDAAEHLALLSSGRLDSVSMRSRYRKEADAIIHASPILPGVVGLIEQAREQ
                     GLQVAIGSSSPHSWVDTHAKRLGIFHYFKYIICQDDVAPGRTKPNPDIYLKALEVLKV
                     QNTEAVVFEDSPNGVLAARRAGIFAVAVPNPLTKRLGVSGDLTVPSLAGISLHEISQA
                     ISLRS"
     gene            13776..15014
                     /locus_tag="PL000_03295"
     CDS             13776..15014
                     /locus_tag="PL000_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87949.1"
                     /translation="MSSFQKKIGLIAVLAFALAGLLFFRPSRTTPPADSPTVMVVDVT
                     ATSTWTPSRIPENIPIADDPLKESILAAGDYLVRQQLTNGELSYQVDFISGERSFSPS
                     HVRLISGTGALFTVCRVSGDSKYCDAADLALDHYLELLVTDPAHFKGTCLFTEGTCQL
                     GGAAMTVDAIYKRWQASGELILHERNLLETAINLGYFIVSMRRPEGGFYHAYDPYFGG
                     IVDDDFYLSYFPGQSLVALTQLYEITGNDFWLEQAREVNDFMITQPISEDHWHSYGLS
                     MLARWDALTEADLTYAKQIADVVIAGEVRSLNLKNTSMATATKIEALSALAQAFYLSN
                     TDHAKLEPEIRAFITFVQARQLPDNNCGWTISEEITEKFGGGFFSNCEDTTIRVDGQQ
                     NWINGVTAYLEYRSMIKARK"
     gene            15099..16109
                     /locus_tag="PL000_03300"
     CDS             15099..16109
                     /locus_tag="PL000_03300"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC87950.1"
                     /translation="MSIGKIKWQSIVSQYAYPETWRSLWQVVNSVIPFFVLWYLAYRS
                     LEIGYWLTLLLTIPAAGFMVRMFIIFHDCCHGSFFETPLANNRLGLLLGVAVWTPYYA
                     WKHSHAIHHATAGDLDRRGIGDVYTMTVEEYLASPWYKKFGYRVMRNPFILFTIGALI
                     VFVFAHRFWEKGAGKREKNSVVWTNIALLGFVGWTIYHIGWQAYLLVEIPILLMACGA
                     GVWLFYVQHNFEPTYWERHQNWEFFKAGMDGSSFYKLPKVLQWFTGNIGFHHIHHLSP
                     KIPNYKLEKCHNENPIFQIQPLTFRDSLKSLYFRLWDEKEKMLVGWRAIKKYKMKMQM
                     DN"
     gene            complement(16291..17781)
                     /locus_tag="PL000_03305"
     CDS             complement(16291..17781)
                     /locus_tag="PL000_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNC87951.1"
                     /translation="MKKNKRTPQSRLKSAFLAGLFILAACAQPTPAPTETSAPIADPA
                     PYSATIFEVKSDAKASPSRLADERPNIIFVLTDDQPYETIQYMPTVRDVLLKNGVNFE
                     NGFVTTPLCCPSRSSMLTGQYAHNHQVLTNRMPLGGAPKLDDSSTIATWMNDAGYRTG
                     YFGKYLNSYEDLKPYGYVPPGWDEWGTLLDAAFYNYNLSVNGTVKAYGETPEEFCADV
                     TTRNAVDFITASKDEPFFMVVGYFNPHSPYFWAERHDEQFRANSALKAEPYRNPAFME
                     EDRSDKPKYLQDLNSIAVSKIDITYKQILRSLLSVDDGVASILNALDKTGLSEKTIIV
                     YLSDNGLTVGDHGFGLTKNCAYESCIRVPFIMYAPGMFPARTESRFVANIDLAPTFAD
                     LAGFAAPDTVDGTSLLPLLKNESITWRDDLLFEHWPTEDGVGADIPEFYAVRNAEWKY
                     VEYSTGEKELYDLVNDPYELNNLAGQPEYAEVQTQLQQRLIELKEE"
     gene            complement(17925..19766)
                     /locus_tag="PL000_03310"
     CDS             complement(17925..19766)
                     /locus_tag="PL000_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC87952.1"
                     /translation="MKEKNSPASAWRSVQIGLAVLLGLFVYAYGFDVTKVNLEELRSE
                     RRQESLTRVTRTLAQPDIIGYDQEKAIVNLPVFVPCPSDDLLATLPEADTSGAYLTVS
                     PACAAPGESVQVEGFNFVAHTEGPIRFVPGNDPANVVELGRETVETDGSGHFSTSFVM
                     PERLSDAPQFIRVTISQNVGAPHFTQNAKDTWDKIIETVFMALLATTAGTILAFPISF
                     LAARNLMKSVRSPLTSVSLSVLGWPIGMGLGYFIVNLVGKLAIPLSENIAISLISVVA
                     TPFIFWYAMRWALPQEETKIPELAMRLTRMLVIFLAILVAFFGLFQLAGFTTSLSLAI
                     EESLGVLGFLATFLFQVSDIVRISIPALGALAAGGALSNFLGRIGQHTAEKSPAARVK
                     SINFIVAALAGATIFGLSGWLVEWLYQIGRVELTFWGPIIIGALLGLLLAFFTKPKDT
                     LPTGMVIYSITRTILNALRSVEALVMAIVFVIAVGIGPFAGMMALGLHTIVSLAKLYS
                     EQVESIMPGPLEAITATGANRLQTIIYAVIPQIVPPYISYTMYRWDINVRMSTIIGFV
                     GGGGIGFLLQQNINLLNYRAASTQMLAIAIVVASMDYISSVLREKYV"
     gene            complement(19770..20522)
                     /gene="phnC"
                     /locus_tag="PL000_03315"
     CDS             complement(19770..20522)
                     /gene="phnC"
                     /locus_tag="PL000_03315"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HNC87953.1"
                     /translation="MLQVKNLTKIYDGGVKALDNVSFEVPKGQFLAVIGLSGSGKSTL
                     LRCINRLIDPTEGQILWDGEDVTAATPEEMRLIRRKIGMVFQHFNLVHRSKVITNVLA
                     GRLGYVNPAWSFLNRFPKEDIEKAIKQLERVGIADKADQRADELSGGQQQRVGIARAL
                     MQDPNMILADEPVASLDPVLAHSIMKHLEEINKKDGVTVLCSLHFLDLVHRYADRVIA
                     LNQGQLVFEGHPRDINDMKFKEIYGRDAERVG"
     gene            complement(20702..21844)
                     /gene="phnD"
                     /locus_tag="PL000_03320"
     CDS             complement(20702..21844)
                     /gene="phnD"
                     /locus_tag="PL000_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC87954.1"
                     /translation="MNKRIAFVLLTLVVAISMMLSACGPAATEAPVATEAPTEAPTEA
                     PTEAPVATEPPMPAIGSPEHPIKVLFVPSVDANIIVTGGEVMAAALNEATGLTFEVVV
                     PTSYAATIEEMCASPSDTMAFIPGLGYAIASQLCGVDVSFKAIRFGFPVYWAEYIVAR
                     DSKYQTLADLEGAKWGYGDQGSTSGYMVPTVELAAAGITPGEQVQTGGHNQTVTAVYN
                     GEVDFGTVFYSVPLTPEGAPLFSYDDFVAGKITSLEQYEIPTESVPNCAPDTEGKKLL
                     CDGWRILDARANIRTEAPDVIQKVRILGVSAAIPNDTLSFGPEFPADLRAQIEEALLA
                     FAATEAWGTSIGSADFYGWSGIAPATDAEYDMVRAMVAATGYEPPK"
     gene            complement(21991..22500)
                     /locus_tag="PL000_03325"
     CDS             complement(21991..22500)
                     /locus_tag="PL000_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC87955.1"
                     /translation="MNILETPRLILRNANADDAPFMLDLLNQPSFIRFIGDRGIRSLD
                     GAREYIETRLVKSYKDNGFGLYITLLKEENLPIGLCGLVKRDGLDDIDIGFAFLAQHE
                     SKGYGFESASAVMEYSKNTLGLKRIAGITTPDNEKSIGLLEKLGLKFEKMIKLPNDTE
                     ELKFFIRTL"
     gene            complement(22634..23674)
                     /locus_tag="PL000_03330"
     CDS             complement(22634..23674)
                     /locus_tag="PL000_03330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC87956.1"
                     /translation="MYTTPKHIFLRSLLLAAYILALVIQPFSARPVFAAPSPQTINYQ
                     PTLTATPASGPAPLTVNFSRYVATDPNGPLVSYVMLDFGAGPGLGAEIDFGDGTSTFI
                     LDKTSSMVYNLPGTYTAVMTVFYNNSTSITTTLIITVDPSVTPEQLPLIVASPASGTA
                     PLVVNLTSSATMNPNGPLINNYKVDYGDGTSDLYFFLDGAPSFINNGSHTYNTPGTYT
                     ATLTVTYNNATSASATTTIIVNPVLSNVVRSTAVNLSATLKKTTVNVSGTVIVQNENG
                     IVIPGAIVSITWVKPNGRVVTQNAKTNANGVAKFSTSGNRGTYTLTVTNIRKTGYSFD
                     SINSVVSGSITK"
     gene            complement(23896..24834)
                     /gene="lipA"
                     /locus_tag="PL000_03335"
     CDS             complement(23896..24834)
                     /gene="lipA"
                     /locus_tag="PL000_03335"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC87957.1"
                     /translation="MPATPTRDRVKPETEARPLRRPEWIKVRAPSGETFERLQVLMRS
                     KELHTVCEEAMCPNLGECWGSGTATFLMLGDVCTRTCAFCDVKHGKPLLMDWGEAERV
                     AQAVKSMNLKHAVITSVNRDERRDGGAPIFAMVIRRIREVLPGCSIEVLIPDFKGSIE
                     ALKIVMDARPEILNHNVETVPRLFKTVQPQDNYEWAAATLSNAKKLDPDVLTKSGIML
                     GLGETMDEVKQVMRDQRSWGVDILTIGQYLQPSKKHMPMDRYYTMEEFAELKAFGMEI
                     GFKWVESNPLVRSSYHAAEQVRALSIVHRKLYGEKA"
     gene            complement(24872..26182)
                     /locus_tag="PL000_03340"
     CDS             complement(24872..26182)
                     /locus_tag="PL000_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNC87958.1"
                     /translation="MKLIRIFLFTLMIALSAFQLAAAQGDDPLAVVMTAEGTIMPPML
                     EYFKRGIETAENSNADVLVIQLNTPGGSIETMLDIIQTMRASKVPIVVYVSPNDAMAG
                     SAGALITMAGHASAMAPKTAIGAASPIDSSGADIESTLETKIKEILKAKARVLVEARG
                     EEATRLAEAMIDDAKAVTASEALEAKLIDFVSDDTEDLLQALNGFTVKMDDGSEVTLN
                     TENIRTEELGISFIEQLLMMLTDPNIAFILLAIGVQGILIEISSPGGWFAGFLGAVCL
                     TLAVYGMGVLTVNWFGFIFLVIAFVLFILDIKAAAHGALTTAGVASFIVGALVLFNSP
                     GTPQFQRVSVPLVIGVGVFIGALFFAIVLVALRSRHNPIQTGVESLLGKTGTAKTFEE
                     EAGQVQVGSELWSAEKAPGSESIRKGDPVEVVEVRGLRLIVKKK"
     gene            complement(26293..29607)
                     /locus_tag="PL000_03345"
     CDS             complement(26293..29607)
                     /locus_tag="PL000_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC87959.1"
                     /translation="MGSLSTLPASIAFPAPPTLAGWAVWLSLLGMLAYALYHWRAYQP
                     AMKRIEWGFLVGLLLLTLVANVFILRPFASSARPLPNLPVQPPGSALIVFSAIPWLLS
                     GGLIGPLGAAAVGALAGLLRGTLDTYSLFSIIEFALMGAWFSTNTRQRFRTPVYSLLR
                     QPIVGALLLIPLHALFYIVVALFTQWGVDIPTTARLDFAISNTGIVTLAFGIEMLVGG
                     IVAQIISMSFPAVWGGKQQALQPSPGEKSLESRFQFAVGTFISLLLLTLLIGDWVVAG
                     QAARDMLQDRLTSAGEAAAQSIPFFLETGQNLAVQLAADPRLLEATGDELHSIIGSRI
                     QAVPYFDQFFVLEANSETLLAGYPDDSVTTFKLYPDESMGLILAANGVLTQIYSIPPI
                     SADESARVSFLVGIVDKFGSVQRVLIGRTTLKTNPLTLPLIEGIDNMKNLNGAGFLLD
                     ESNRIIYHSETIEMAATYNGQRSTEAVFYDDTAADGTRQLVYYQPVTGRPWAVVLTVP
                     AQQAQQLALNIAMPLSLMILVLAVVAMISLRVGLRVVTGSLQSLATEANRIAQGNFDH
                     PLQTEGVDEVGQLRRAFEKMRSSLQARLNEINSLLRVSQGVASSLEMQDAVKPVLDAI
                     LATGANSVSVVLSPSILPDTFVELPSRFAVGTVQDIYAHLDDQILALAQSQEKVVLPN
                     LSRSRELELDPSLPQPLSLLAVALRHENRYYGVVWAGFEQPRMFSDSDIRFMTTLAGQ
                     AALAVANAHLFLNVEASRRQLKAILNSTPDPVLVTDHRNRLLLTNRAAAIALGQSEDT
                     TSDIGRKTEQVVKIRPLLALLESATTENQSTEITLADKRTYLATASAVMVEGKQIGRV
                     CIMRDVTHFKELDMMKSEFVATVSHDLRSPLTLMRGYATMLEMVGELNEQQQGYVKKI
                     ISGVENMSRLVNNLLDLGRIDLGVGLQVENVSVLDIIERVTGALQLQASQKNIQLTVE
                     LSKDMPHAIEADQALLHQAVYNLVENAIKYTPNDQRVTIRTETQPGSLTFIVEDSGIG
                     IAADDLPRLFEKFYRGKQREARAQHGSGLGLAIVHSIATNHGGKVWVDSVVGKGSTFY
                     LQIPLTQPKENKRIGP"
     gene            complement(29683..30429)
                     /locus_tag="PL000_03350"
     CDS             complement(29683..30429)
                     /locus_tag="PL000_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC87960.1"
                     /translation="MDTPTPKPVSEVWRPELVRLPKMTHARRAFRVFGHGLTKLVAKL
                     CLHVTVEGLENFPRKAGAEPVLVVINHLGDSDVPALISSLPFPPDALAKVELYDLPIL
                     GKLMDWYGVIWLHRGRPDKRALRAALDGLAEGRVIVIAPEGRYSLTGALEEGNNGAAF
                     LAYKSGAAVLPIAVTGTENETVYGNLKKFRRARVNLRVGKMFKLAGQAQGGERGGAGE
                     RQEVIAQGTRQIMEALAGLLPEKYRGAYSG"
     gene            complement(30432..31136)
                     /locus_tag="PL000_03355"
     CDS             complement(30432..31136)
                     /locus_tag="PL000_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC87961.1"
                     /translation="MQVKDYRIPFHIQANRLWLRFGIRTLFNILGSVKITGAENIPLG
                     SPYVIAMNHISIFDPPLVVSFWPEEPEVIGASDVFAKKGQGTILSLYGVIPVHRGTYD
                     RTLLEKIILILKQGRPLVIAPEGGRSHEPAMRRAMPGVGYIIEHAGVPVVPVGLVGTT
                     DDFWQRSKQYWRGQGEKPRLEMRIGKPIHFQPILEKGAERREARQKNADLVMQHVAGL
                     LPAEYHGVYTGQAIST"
     gene            complement(31127..31810)
                     /gene="cmk"
                     /locus_tag="PL000_03360"
     CDS             complement(31127..31810)
                     /gene="cmk"
                     /locus_tag="PL000_03360"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC87962.1"
                     /translation="MTNKPTPSIIALDGPAASGKSTLGKRLADALGFLFFDTGIMYRA
                     ITWIALQHDMDLRNEAAVSQLAQNAQIDIRPPSKNDGRACDVIIGEKDVTWDMRGGDV
                     DANVSVVSAYAKVRQALSEQQRRIGMRGNVVMVGRDIGTVVLPNADLKIYLDASAEER
                     ARRRYNEIIARGEPANLDEILKKVIERDRIDSTRAVAPLRPADDAILIDSDAMDAEQV
                     FAYVLNLCK"
     gene            complement(31825..33510)
                     /locus_tag="PL000_03365"
     CDS             complement(31825..33510)
                     /locus_tag="PL000_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87963.1"
                     /translation="MIYRHFKHETWLYVLVLLIAVSVRTIQLGAMPLSDSEAAPALQA
                     LQITQGQRPLLSPHPFYILSTAVLFFLYGEGTNFLARFSPALIGSLLVLIPLLFTERI
                     KPRPGLILALFIALDPGMVALSRQAASPIFAITFLLFAIGFYENNNLKLAGSFAALAL
                     LGGPSIWLGLLSIGIAWAIYRGVQLRGSEEQASVNVKEALWPFVVTLIVAGTFFFLIP
                     NGLSAAFSSIPEFFKRWTIASDFPASRFLIALAVYQPLGLLLAVIALIRGWQHGSRRI
                     ISLSLWFIVALLLAIFIPSREIADISWALIPLWGLAALELIRNLHIFPEERNEIFGVV
                     FLTAFIWVFAWLDFSGMVWLPNTTREYMMRFWLLVGALLLIAISLLLVAAGWSIRTAR
                     IGGVLGMALVLGALGLGGTFGAAGLRGLDNPEIWWQAPLPVQAKLIQASVNDLSEWGT
                     GNDNMAPVIIAGLDSPALEWALREHQVEVVETLDISSAPYFVITPLQNDPAFASTYRG
                     QDFTWRKTFLWNETLPQDWIRWVTLREVPQTGESIILWARDDLFLDKANQSIP"
     gene            complement(33589..37506)
                     /locus_tag="PL000_03370"
     CDS             complement(33589..37506)
                     /locus_tag="PL000_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC87964.1"
                     /translation="MSLQEPSQKPDPFSDKDAFNLLFENHPTPMWIHDSKTLALLDVN
                     EAAQQLYGYPHEEFLSITLKEIYAVTEMPVDSESLTGEFQHRKKNGQVIHVEVTLRTI
                     QYGGQNAVLAVIKDLTKQYDQLYLRISALNAAANAIVITDTNGMIEWVNPAFSTLTGY
                     TFEEALNKNPRDLIKSGEHEPGFYKTMWSTILSGKVWHGEVINRRKDGSLYDEEQTIT
                     PLKDDNGKITHFISIKQDISQRKRTEKIVNQWLAEQNTLHQVSQSLLSIHLDSDMIYS
                     TLHEAVVQIMPCDVFTIVLDDKGNGDYHAVYLYDLEERFPPRRVPRGQGLSGQVISSG
                     QTLFIHDNFEARTPLIRFGTPNEVRSILAVPLRKHRKTVGMISVQSYQPNVYGERHRV
                     LLETLAAQATTTIENASLFAETRQRLVELELLYESGLALNHLLNPKELGTKIVELLEQ
                     KLGWHHITIRLTRPQDDGLELLAFKQAGLTDEVEKEKVAERFQALIQHIGQGLSGWAI
                     EHQQVVRVDELSNDPRYVDTYPGLHSGLYVPMKLGERIIGVISIESELPNAFSEADER
                     LTATLANQAASAIENARLYEAERKQHRMSDALRDALHAGALMSTSLDFETILDRLLET
                     VKRIVPFAGGTMMFVDHEQRRVRITKMHGYKMLKKEMIEKISSYSFDLSTAENLRWML
                     DNKQPLVISDVRQYPGWVRIPESEFIRSWLGAPIIINGQVIALFSLDSTETNFFTEEH
                     AELLQAFTGQASLALQNARLFDETAQRALEFASLYQTSSFLSAENELDSLLQNIVESA
                     KKLLNSNSSGIYLYHPEDEELELSVITTNDILTGVRLRLGEGVAGIVAQTHQPLRIEN
                     YSEWEGRSSKYDELRIRAVLEVPMLYGGELIGVLTADEINDSTRVFTESDERLLTLFA
                     SQAAGAIHSARLREQTAQRVNQLQALHTIDRAISSSFDLRLILNTVISQSISQLGVDA
                     ADILLFNPYLQTLEYVAGQGFRTRNIEQSRLRLGEGQGGQVALNRTVLHVSNLVKAGT
                     EFTRASLLVGEGFLEYYGVPLIAKGEIKGVLEVFHRTPLHASSDWLGFLETLAGQAAI
                     TIDQTQLFDDLQRANLELIIAYDTTIEGWSHAMDLRDEETEGHTRRVTEMTLALANAM
                     GISDDQMLHIRRGALLHDIGKMGIPDKILLKEEKLTEAEWKVMRQHPNYARDMLQPIK
                     YLQKSLDIPYCHHEKWDGSGYPRGLKGEQIPLVARIFAIIDVWDAVTIDRPYRKAWTK
                     QKALKYIREQSGKHFDPSVVAAFLKMFDKKDPPAGKPAKRKNK"
     gene            complement(37506..39233)
                     /locus_tag="PL000_03375"
     CDS             complement(37506..39233)
                     /locus_tag="PL000_03375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC87965.1"
                     /translation="MPRNAHPKSQAKSADEKQTFYLLFMGHPTPMYVCDAQTYEILEA
                     NEAAVNSYLYSREDFRKLNLHDVLQADAVTRISADGGKVQQDGHSYKKLQHKRKDMES
                     VEVEAETHMIEFDGRAAALIMVKPAMPTQAVENQSESELELRALFASMQDVVLVIDHD
                     GVYRKVAPTNSELLIKPKSELLGKNLSEFFPAERAEFFLKSIRQVLETRQSTHIEYEI
                     SLEYGTIWFAATISPMDENNTLWVVRDITERKMVNKALQESEGRYRSLFDRMMDGIYR
                     STHEGKFVDVNPAMVKMFGYSSKEEMLAIDIKTELYFDAEERGTHKLDTGDGETDIYR
                     LRRKDGTEIWVEDRGSYTYDDHGNVIYHEGTLRDVTARKHAEEALHRAKESLERTNQE
                     LERVLQREQQLARTDGLTGLYNYRYFFELATREFNAAMRYRRPLSILMFDTDDFKRIN
                     DTLGHSAGDKILALIAQATAAQMRSVDVLARYGGDEFIILLPQTSAKQAYPIAERIRN
                     SVADMQMPSDKGMVSVTLSIGIAEIQYEPADESVEFIVQRADKALYAVKQAGRNRAMI
                     YTASLDGEN"
     gene            complement(39442..40698)
                     /locus_tag="PL000_03380"
     CDS             complement(39442..40698)
                     /locus_tag="PL000_03380"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HNC87966.1"
                     /translation="MDTISRITFRRYLLGQQGLWPGRRFKGLAGTTSAIQQMEGIQLD
                     PLNIVARSQEIALYGRVLDFKPENLYQAAYEKRGFFDYGGWLFMYPMSEFPYWRLHMK
                     HRKKQGIYYHDAYQQPPAKLLNAVLNELRTRGPLGNRDFSGEALKSWSYRGRKEESIA
                     LFYLWLIGEVMITNRKGFDRIYDLTERVLPKKYEHTVPDSEAEDFFSRKTIAHLGLAR
                     ENVWRTGLAGFIHRDVSREEGKKRIERLIEQGIASRLRVEGSKDGYLVLNSEQSHLSD
                     LEAGRIPKKWKLLDTSTQEEVTFLAPLEMVSARGRAKVVFDFEYLWEVYKPLHQRRWG
                     YYTLPILYGDDLVARLDPKLDRQTETLHILGFWLEDDAPKDEAFASALAKGLARFARM
                     IRAKKVDVSAVRPPKLRSYLKKNIGL"
     gene            complement(40752..40943)
                     /locus_tag="PL000_03385"
     CDS             complement(40752..40943)
                     /locus_tag="PL000_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87967.1"
                     /translation="MNNSKNILLGALIGAVTGLIAAMLLTRRAEKNERETALTAGEGL
                     KLGVLIFGLLRAIASLGDD"
     gene            complement(40940..41368)
                     /locus_tag="PL000_03390"
     CDS             complement(40940..41368)
                     /locus_tag="PL000_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87968.1"
                     /translation="MKAQLPHPEHYSYLVHRKQRNTQVILPVVLSALLMIGMIVLISF
                     ATFKSGGDVGRWAAISTIWIIIPLLITGLIVLALLIGGIYLMARALSGLPYYTGIAQD
                     YVHKARGYITRGADMAAKPILAINDLVENLKAFFGRISNS"
     gene            complement(41417..42160)
                     /locus_tag="PL000_03395"
     CDS             complement(41417..42160)
                     /locus_tag="PL000_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HNC87969.1"
                     /translation="MKKLLIIASLLLGACANQPQEAPHWTYEGEEGPQHWGEIDPSYA
                     VCGTGENQSPIDISKPIEKDLVNISFHYQPSEVNILNNGHTAQVNYDTGSYIELDGTR
                     YDVVQFHYHAPSEHHIDGQAFAAEIHIVHKNAEGKLAVIGILLQQGTENAAFDPFVNN
                     LPAEKTDAKDAGSKVNAADFLPAEQTTFRYSGSLTTPPCSEGVSWLVMTSPVEVSAAQ
                     LEKLLTVFEGNNRPLQALNERTPLEDSTP"
     gene            complement(42421..42885)
                     /locus_tag="PL000_03400"
     CDS             complement(42421..42885)
                     /locus_tag="PL000_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC87970.1"
                     /translation="MSKKKNRNTTSRSGLSAFLRKPAGQLTLIAVVVIVIVLIALSGQ
                     GSGQGLSREVNTDQAYEMYQNGAFVVDVRTQEEWDEYHAPNTTLIPLDQLQSRLAEIP
                     KDREILVVCRSGNRSQQGRDILLAAGYNATSMAGGLKEWYAKGYPVEGKPAQ"
     gene            complement(43070..43480)
                     /locus_tag="PL000_03405"
     CDS             complement(43070..43480)
                     /locus_tag="PL000_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC87971.1"
                     /translation="MQIRITKQEWRVFVTIMHLSGNLDASNYIEVINKAQELYASGVR
                     DILIDLSGVSYVSSAGLMSLHTVVLIFAGQSMYSKETGRPSFRAINFERDGAVRQHVK
                     LFGPQPSVEQVLDVVGLKQFFDVYHDLDAAVKSY"
     gene            complement(43513..44790)
                     /locus_tag="PL000_03410"
     CDS             complement(43513..44790)
                     /locus_tag="PL000_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HNC87972.1"
                     /translation="MKKKNIGNLTIIILVALTLIIWIVFPPVAEGAEHYARAYAGEVL
                     GSLVIVLMSFSLFISTRPKWAEPFFGGLDKMYMTHRHTSTSAFLLMFVHLLTVPITLV
                     NLRVGNYLAMVAFLGIVAIVLPTLAPRIPFLSKLTGQTYEGWKKLHRFVGIFFILGFV
                     HSLTINAPSAKIAITWTQNFFILGTLSYLYTEVFGGLFKKYLPYTVEAVNHPNNSTTE
                     VVLRAKKESIKNQKAGQFLFVHFAGEGLDEAHPFTISSAPHEDVLRVTIKACGDFTRH
                     LFANLKAGMSATVEGSYGMFDYKSGGPKQIWIAGGIGITPFLSFIRDLKTDPAFDVDF
                     YYTVRHREEAIFVEEIQTAAQKYPRLKTHIRFSSTDGSLTVEEIAKNAGGDISTHHVY
                     MCGPLPMVQAFEAKFIEMKVPADNIHFEEFNFR"
     gene            complement(44869..45279)
                     /locus_tag="PL000_03415"
     CDS             complement(44869..45279)
                     /locus_tag="PL000_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC87973.1"
                     /translation="MEITISQEQGRISVAVIKLAGHLDGQTYQTLIAKAREVVEAGTR
                     NILLDLGDLTYISSAGLVSLHTIALMTRGEELPDLEHGWATLKSMDRSRDGGMQKNLK
                     LLNPRPEVISVLDMVGFSAFFETFTDRQKAIDSF"
     gene            complement(45311..46549)
                     /locus_tag="PL000_03420"
     CDS             complement(45311..46549)
                     /locus_tag="PL000_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC87974.1"
                     /translation="MDTIHPMSSLADKLKALGVKTGTADLPAPKPASRSIDSVVAGTF
                     FQTPRGEAFIAEQTFAEDYLHGKTAYASSFPFSIIAQWANDPRMAELPIHKFAFLDTE
                     TSGMAGGTGTYAFLVGAGRFVDGRFVLRQFFLRDPSEEPAMLEALIDFLAPCEGLVTF
                     NGKAFDAPLLVTRYSLHRIPVPFKDYAHLDLLPLARRLWRDRLPSRALKYLEEHVLGF
                     TRTSEEVPGYEIPWLYFDYLRTGDASPMAGVFYHNAMDVVAMAALLAHVSELLADPYN
                     GRVEHGLDFIALGKLFEDLGHWDEAARLFERGLELGLNESDFGVAVKRLSILQKKRGD
                     ADQAIRLWEQAAQRGHVYAHIELAKYYEHKMRDAETSLKWARSARAEVERADLPPYIR
                     KHWLGEIDHRLQRLERKAGL"
     gene            46641..46844
                     /locus_tag="PL000_03425"
     CDS             46641..46844
                     /locus_tag="PL000_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87975.1"
                     /translation="MVKVNYQYEKRKKELDKKRKKEQKLKLKQQKKNNPSGEVLTEVQ
                     DGAMVEAPAETMPVTPPVEEVDK"
     gene            47019..47795
                     /locus_tag="PL000_03430"
     CDS             47019..47795
                     /locus_tag="PL000_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87976.1"
                     /translation="MNRSLNLQLKLLPFLKSASLKRGAIFGGILISALLAFELFNFGT
                     TSFALQDVLGNLKFAGIQWSAILAFAFCGIDFAGIARIFTPEQGRDEPAEVWYLFGAW
                     LLAAGFNAMLTWWGVSVAILNHNAEGGSLVGQAVMVKAVPIFVAVMVWLIRVLIIGTF
                     SIAGERLFSLAEATSRPAATAYQNNSPRPVTQSQPVYRPAAMSASSSNQNYPRPAPKP
                     RPAPVSPNYGANRPEPTYHPIGMTAMGHGEGSNSTSSVRR"
     gene            complement(47901..48779)
                     /locus_tag="PL000_03435"
     CDS             complement(47901..48779)
                     /locus_tag="PL000_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87977.1"
                     /translation="MNNFSIRLIETPEEMRLVEDLQRDVWPGSETDVVPLHMLITAVH
                     NGGLVLGAFVEEKIVGFVFGFPGLESTPDGPRPKHCSHMMGIHPDYRDGGVGFSLKRA
                     QWQMVRHQDLDHVTWTYDPLLSRNAYLNISKLGAVCVTYRRSEYGDMRDGLNAGLPSD
                     RFQVDWWINTPRVERRLSKRPRPTLKLSHVASSGLRQFYPLRTSTNGLPQPPEHVPPF
                     DDRLLLAEIPSDFTALKSQDFALARDWRFFTRELFETAFEKNFIVTDFVFDPNNGTPR
                     GLYILTHGESTLDEFE"
     gene            complement(48782..>49796)
                     /locus_tag="PL000_03440"
     CDS             complement(48782..>49796)
                     /locus_tag="PL000_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC87978.1"
                     /translation="SNWGSSATRQRLRPSAIIPAMILITGATGFIGRTLVRQLSSIGY
                     PLRALIRPSPRTPNLPKGVPVEVAVVSLADKRGLRAAMRDVEAVIHLASAENQGSRGN
                     LLTADIQGTENVVEAAADAGVDRIVYLSHIGAARSSGYPAFRAKGIAEEHIRNGKVPY
                     TILRSSLVYGPEDHFTNNLSRLVRASVGLFPIQSGGRTVVQPVWVEDLATCILWSLEN
                     EDTLNRVYELGGSEFFTLQQIIETIMDVTRQRRVLVPLSPITLRAITVFLDGVIPNFP
                     ISSFWLDYFAVNRTCAVDSMPRVFGLMPARFTYRLDYLIRKPWYQRAWNTLTERIAAL
                     RTR"
BASE COUNT        11695 a        12177 c        13237 g        12687 t
ORIGIN      
        1 ggctcatggg tgttgacctg gagtccatag aaaaacaaac ctcgccagca ttattcggcg
       61 aggtttgttt ttttgatttt ctttttttaa tccaaatgtc cttcgaggca caccacacct
      121 tcggggccga cctgtgcatc gtggaccgta cccgctggca ggtcgaggcg atcgcctgtg
      181 tgcagggtga tctgcttttt gagcaggggc aatccaaagg taatgctgcc ccgtaccaca
      241 tagatcacct tgtcgaaacc atgtttgtgt gccgagtaaa catcatgcgg gccgtttgac
      301 catgagtagg gattcaatcc ctgccctgcc atgatcgcaa ataacaattc ctcggttggc
      361 gggctggctg attcccacgg ggtgacgcga aacgaattta ttgtcatcct gttttttcca
      421 tcaattcaat gcgatttccg aacgggtcga agacatgcgc ccgctgataa ccttccagcg
      481 gtggttgggt tgtatctgtt tcaaattttg cccgctgtat ttttgcgagc aactcatcga
      541 gacctctcac aatgaaagca gggtgcgcct tgcgcgcggg atggaaatct gattcgacgc
      601 cgagatgtaa cttgacagaa cccgcttcga gccagagtcc gccgcgctgt gccaattctg
      661 gcggttttgg aacttcacga aatccaagca gcccgatgaa gaaagaccgc gcttcacgct
      721 ccgcgtcgcc gccagctggt attgcgattt ggacatggtc aaccccgaga atgctcattt
      781 tattccgtcg gatatccagc atcgatccag gcggataatc caccaaggat cggggtcact
      841 ttgacaaaac cgttgtgcaa catgaggaac gccgcacggg cgctcgtgtg ttcgttttgt
      901 caggtgcagt aggtgatgat ccatttgttt ttatcgaggg aaacgttggc aatatcgcct
      961 tcaaaatttc ccaatggcac caaaacggcg cccgcaatgt gagcctctgc gtaggcttcc
     1021 ggtccacgta catccacgat gatggcttcg ccgctatcga gggcggcttt ggcctcttca
     1081 gcggttacac gcggaacagc cgcttctgat tcgggcaatc tgctgttggc tgttggtcgt
     1141 ggttcgagtg tgaagatcgg tgcctgtgtt gaaatggggg catcgggttc attttgcggc
     1201 agaatggcat tgcatgccag cactgtcaac aaagcaaccg aaagaccgat aaaaatgttc
     1261 ctttgtcttg tcatttacct acctccaaag tttatttaaa gtgcgccgca tcagacggat
     1321 caactctcac gaatctgatg cgacgcaccc tgcctgatac gattacaact cgcagaaacg
     1381 gtcaacgcgt ttgccaatga tggcgaggct gtcatcccag aatttgcggg tggtgatatc
     1441 aatgccgaaa cgcttggcga gtttcgctgc actttcctcg cctgtggatg caagcaggtt
     1501 cttgtaatct tccacaaagt ccttgccgcg ttgttgataa atggcgtaca accccgtagc
     1561 gaacaagagg ccgaaggcat acgggaaatt gtaaaaggaa agcccggaag aatagtaatg
     1621 cggcttccag gtccacatga atttttgcaa gtatttctcg tcaatgccgt caccataggt
     1681 ggctttttgg gcacgttcca tgatgtcatt gaaatcgtcg gcggagagtt ctgatttttc
     1741 gcggcgttcg aacacttcct tttcgaagag gaagcgcgaa taaatatcca ccaccacctg
     1801 gcttgcgcct tgaatctgtg cttcaagcac ggccaactct tcctgtgggt ctttggttgt
     1861 ggagagtacg gcctcggtga cgatcgtttc gcacatggtt gaggcggttt cggccagggt
     1921 catgggcgtg agttgctgca gcaccgtctt atctgcctga taggcgcatt cgttgtggaa
     1981 ggcatgtccc aattcgtggg ccaacgtgct gatctgatcg aacgaaccgt caaaattgca
     2041 gaggatacgg ctttccttta acgcctgcac ttcagtgcag aatgcaccgc cgcgcttgcc
     2101 ttcacgttgt tcggcgtcga tccaattgtt gtcgaatgcg cgtttggcga atgtgctcaa
     2161 atcgggtgag aatttttcaa aattctcaag aatgaaatcg cgtgcctctt cgtaggaata
     2221 caccttgtct gtctttccga gcggggcaaa gatatcccac caggcgagtt gttcctttcc
     2281 gatcttcttg gccttggctt tgaagtactt gcggaacatc gggaaggaat ccttcattgc
     2341 ttcgagcatg gcttcgagcg tcttgcggtc gaggcgggcg gcttcaatgg ccgagtggag
     2401 tgcatccttg cgtccgcggc gtttattcaa gactataact tcgcctttaa ctccattgag
     2461 acaggctgcc agcgtttctt ttacaccttc ccaagcctga ttttccgctt catacccgcg
     2521 gcggcggacg gattcatcag ggtgggaacg caggttgatt aaggctggca ttggcagttt
     2581 ttccactttg ccattgagtt caaattccac tgacatttgc gaaacgaccg ctccctgtag
     2641 ttttccgaag gcattcccac cgctgagcga catttctgcc gcaagctgct cttcgatctc
     2701 actcatcagg tatttgcttt gctgtgcaga ttccttcagc atgaaggcgt gtgcctttgc
     2761 agaaggatcc aacttgatga tctcacccaa tttttttgca tttgccccga cccaggcggt
     2821 gaactgtgtg tacagttgac tcatgggcag gctggcctgc tccatttcag aaagggtctt
     2881 catggccacc ttgtcacgcg aattggtggt gacgaatgaa taaatgtaag ggaagatcgt
     2941 ggaagaaagg gtttggatgc tgttgatgcg gtcaatggct tcaccaacaa gcggggctat
     3001 ttcccgtgct tttgtttttg gagtgacttt cccgagcttg ttcttgaaaa actttccaaa
     3061 agatgcgacc tgttttttgt aatcatcaac agcggctttg aattccttcg actcaagcga
     3121 aggatagaca tttgtgaggt cccagtgtgg ggcagatagg gtcattgttt ctccttgatt
     3181 taccagatat ggctagtata ccaaagcgcc attggaactt tttgacttgc cttttcatct
     3241 ttgaacctat acaatgaaat taacacggag gttcttatga aacaggatcg atttcttacc
     3301 ggcattctgg caggcattgg ggttttgata ctgcttgcgc ttgttttgtt ctttgtacgg
     3361 caggacgata cgaaagaata cagggttgat gcttctcctg aagcaaccgt ctataactat
     3421 gttctcgcca tcaccaacga cgattaccaa aaggcttatt cctatctggc ggaaaaggaa
     3481 cacaagccca gctatgaaca atttcggcaa tcctttttta atggcaatgt ttccccggat
     3541 aacgtcggtg tggatgtcgg aacagccgag atcagtgaag atgatgccct ggtgaatcta
     3601 tccttggttt actcccccaa cgacccattc ttcggcggtt atagcagtac agaccgcgcc
     3661 caacttatcc accagaatgg tgaatggaaa ttaacttaca tgccatacaa cttctggtct
     3721 tacgattggt atcaggaaca attcaagtaa cagatgacag gatggagtga aagatgaaaa
     3781 cagtcagaag gctctatttt tacgcggtag ccctaatcag ttttgaggtg gtgttgtggg
     3841 ggctggtcgg cttgatccgc tccatcgtca acccgaacct gatcaccgac aacgcacagg
     3901 cgctggccac agccctgtct ctcattttgg tgggcattcc gtttttcctt atccattggt
     3961 tgtgggccca gcgggtttca gcgcgcgacg aagaggagaa aactgccaca gtccgcgctg
     4021 tttttttgta tggcgcatta tttgccacac tcctgcctgc agttcaaaac ctgttggcac
     4081 tgatcaatcg cacgttcatt ggcgccgcac ggatctccta cgaacgtgcc ctggtcggcg
     4141 gcacccaatc ctggccggat aaccttattg cgatcatcct taacctgttg atggctgctt
     4201 atttctggag cattctccgc gccgaatggc ccaccctgcc cgatgttgaa aatttcaaag
     4261 atatccgccg tgtttatcga tacttgtgga tgctgtacgg attgttgatg gtcgtttttg
     4321 gtacacagca aattttgcaa ttcattttgc agatcccaac agatttgatc ggaacctccg
     4381 gacgtgaaac gtttgtcaac gcaacggcat tgctggtggt tggaacaccg atctggttct
     4441 ttgcctggcg cttcatacaa gacgctttgc ctgatcctga tgaaagcggc tctgttctgc
     4501 gccttgtcat tctctacctt ctgtccatcg gcggaaccat caccgtactg gcagcaggtg
     4561 gaaacatcct ttacgacctg ctcagccgcc ttttcggtga aaacatttca aacaccgacc
     4621 tgctgcaaaa tatcagcgat ccgctttcgt tggtgattcc cttcggcgtt ttgtggggct
     4681 attttggcgg ccaattaaac agacaatttg acgctgaaac aaacattccg cgccgctcag
     4741 ggatgaagcg aatctatttt tatattcttt ccattatcgg gctgggagcc actgtaaccg
     4801 gggtactctc gttgtttgtc gtgatcgtca atcaactcag cggtaaagat gtggatgtca
     4861 ttgcagcgga ttctgttttg aagcaattgg catcggcgat ttctgtgatt gttgtagggc
     4921 tgccgctttg gttgaaagcc tggaatccca tgcaggcaga ggcacttgcc gacggtgaat
     4981 ctggcgggca tgcgcgccgt tccatcactc gaaaatcata tttgtacctg gttttgtttg
     5041 cttccgtgct tggaaccatg gccccggctg ggatgatcat tttcctgtta attcaagcag
     5101 cgctaaaagg cggggttgaa aacgattttc tgaacagtat tttgaatgct tttcaggctt
     5161 tgatcgtgtt tgccgtggtg ttgacgtatc atcttttggc attacgcaag gatggtgcgg
     5221 cacaggctga tgtattggag gagaagcaat ctgaatttcg cgtggtggta tttgaccatg
     5281 acggaaaatt tggggaggct ctcaaggcgg tttttacaag gcatgctccc aaattacctg
     5341 taacggtgtt gatggtgggg cagccaattc ctgttgattt gaaggcgaat gccgtggttt
     5401 tatcaggttc tttggctgta aatttgcccg accatgttga agcgtggttt cgttcattca
     5461 acgggagcag gctcatcgtt aatgatgatg cggcgggcat ttattggatg aatgatttcg
     5521 ggcaggcggc gctatccgca caagcattgg cagaagggca ggagatccgc ccgcgttcgg
     5581 ctgtgaaatc gacacccata tggacgtatg tcgcttacgt gttcgcggca ttgtttgcct
     5641 gtcagttgat ctttattttg ttgtctttgg gtatttcttt ggtggttggc ggcttttgat
     5701 cggcgtgctg tgtttttgac aatgagttaa tgatatactt tcaaaaaaat ccatccgagg
     5761 cgataaccta tgaaagtaac tgtccttcaa gaaaaccttg cacgcggttt gagtactgtc
     5821 tcccgcgccg tttcgccgcg cagcaccctt cctgttttat ccaatatttt gatcgcaagt
     5881 gatgaaggcc gtttgcggct ttctgccacc aaccttgagt tgggcattac ctgttggatc
     5941 gccgcccgta ttgatgagga aggctccact acggtgcctg cccgcacttt tgcagatttg
     6001 gtgaatacac tacccggcga tcaggtgcag cttaatctgg atgtgaaaac acagaatctg
     6061 catgttaaga gcggatcttc caataacgat atcaagtgca ttgatgccca ggagttcccc
     6121 ccattgcccg tgcccgaaat ggacggcgct gtgcaattga atgtggcgga tttcaaggaa
     6181 atgattcagc aggttgtctt tgctgcatcc actgatgagg cccgtcctgt gttaatgggt
     6241 gtgttgatgc atgttgagaa ggacaaactt acgatggccg cagcagacgg tttccgtctt
     6301 tcggtgcgta aggccgtgct ttcgaacccg gtgccgcagc cgttgaattt gatcattcca
     6361 tcacgcgctt tgagcgaatt ggcgcgtgtg gcttcagacc ctgaagaacc catttatatg
     6421 gtcgctccga agaatcgcgg tcaggtgttg ttccgtgtaa aggacgtgga agttgtttcg
     6481 cagttgattg atggaacctt ccctgattat cagcaaatca ttccgcgcag ctacaaatcg
     6541 cggacattgg tctccacggc ttcattactc aaggcttgca agcaggctga aatttttgcg
     6601 cgtgaaggct cgaacgtagc tcgttttgac atcaagcagt cgaatggtga aatgcagccc
     6661 agtgaagttg agatctctgc cacgtcagaa gaaaccggta agaatgagac cattgttgag
     6721 gcaactgtag acggcggcag tgtgttgatc gcgttcaatg tcaaattttt gcgcgaggct
     6781 ttagaggtga tcaagacccc gaatgttgca ttggaaactt ctgcggcgaa tgcacctggt
     6841 gtcatcaaac cagtcgggga cgataatttc ctgcatgtga tcatgccaat gcatttagga
     6901 tagtgttgat atagtcaaaa aaagtccgct gaataaaaca ttcagcggac tttttttgac
     6961 tgctaagtaa aagaacaaat gttctaattt gtaggcgctt cattttttgc gggaaagctg
     7021 gtacgaaatc gaatggcata gatcaatacc ccaccaccta gaatcaagat caggagttcg
     7081 aacaaaaaca cacctgtaat aacattttct ccagctgtga ttttgggaaa tacataaacg
     7141 gctaatgcat caatgaaagc atgccagagc aacgcgatcc aaaaccattt cttttggttg
     7201 gaaataatgc tatacaacac aagaatggat aagccaatgt gcaagcttaa tgctgagata
     7261 cgttccataa atccaaacaa tggcattgca tcaggttgtg cccagtaagc agcaacttgt
     7321 tgttgaacga tgtcaagttg atctgctggt aaattcaaag cagaaagatt gattgggttg
     7381 cgcatggcga tcatttgaat cattgttgag atcgaaatga taccaagcag gatcgcttcg
     7441 atgccgccat gtcctaaccc gataacaatg ctggtttgcc aggtgtccat tgatttgcgg
     7501 atggttctaa acaaaatgta acgggctgtt tcttcaaaaa tacctgcgag caatcccaat
     7561 acaattgcat tgaaaatcag cgttatggat ttatccggcg ctgggagtat gccgctcgaa
     7621 aacaaggcgg tcaatccata cacaacgggt atgtgaagca tctgtgccgc aatgaaagtc
     7681 aacgcgccag ttgtaaataa tttgcgaaag tctttgaatc caaagttgaa atttcgcgca
     7741 attaagaagc cagcaacaat gggcaacaaa atcatgctta tgtaactcag gatggaatat
     7801 gagatgaaca tttttctcca tatctgtata gtttttgtta agtgagtatt tgctcggtag
     7861 tttcacgtga aacaaaaaga tgcggcttct aaactgaagc cgcatctttt tgtttaagcc
     7921 tttatttcat tgcgatacaa aacatatgag tagactacta gaaaaagtgt tgaccctatc
     7981 aacggaacga acattagcca gaatgccagc atgccgccaa aaaatcctcc gatcatggca
     8041 aacactcctg aaagcatgaa taagattgaa ccgagttgat gcgttttact ccagactgtg
     8101 tcactggaaa gagtccatgg agttcgaatt ccgatgaaga agttccgcct ggcttttctc
     8161 aacatgtaac caataaagat gaacatcagg ccgataattg gcagtaaggt tgtgctcatt
     8221 ttgaagttgt tatagccaag actccagatc aaagttagtg tgtggacata gagcatgaac
     8281 atcacaaaga aaacaataaa caggttgaac gtttcgcgga attccacaat attggccttg
     8341 agcggatcca tgttgggaat gaatatgaaa agcgcgaaca ttcccagtgt gacgatgggc
     8401 atcagaaata ctccccaaaa tcttggcatg gtcccatcca cttcatcatt ggcgttccag
     8461 tgagaagcca tttgctcggg gagttgattc caaaggaata caccagccat aatggcaata
     8521 acgatcatgc caagtgcaat tattgaagtt gttttggtat tcattttttc tcttttctac
     8581 ttcgtttcac gtgaaacgat tatttttcct taacctgatt attctgtgca agcgacacca
     8641 ttccacttaa gccgccaagg ttcatcgaat ccactgcgga tgatggaacg attacgagtg
     8701 cgcctttttc tttcaggcct tcaaacagca tattcattgc cctcaaatgc agcgctgttg
     8761 gattgttgat ataggcttgt gacgcctctg caaatgaaat tgcaatttgt tgctccgatt
     8821 ctccaagaat gacgcgtgcc tgtctttccc gttctgcctg tgcttgacgt gacatggcat
     8881 cctctaaaac ctgaggaatg accacgtcac ggatctccac agattgtacg gtcacgcccc
     8941 aaggcgtagt tcgctcgtca ataatttttt gtaattcttc atccattttg gcgcggccca
     9001 ccagaatatc agcaagtgac atttgcccga taatctcgcg caaagcagtt tgagcggccc
     9061 atgagatcgc aacctggtaa tcctttactt caagcgcagc tttttctgca tcccacacca
     9121 cccaaaacag gacagcgtcc acatctacag gtacggtatc tttggtcagg gttttttccg
     9181 ctgaaaaaga tgtgaccatt acacgatggt cgatccacat ggtcgcattg tcaatgatcg
     9241 gaattaacca aaacaaacct gggcctttca gtccatggaa ttttcccagg cgcagtacaa
     9301 cagctttttc ccattgcgat gccaccctca atgcaaaaag gaaatatgcg ccgatcatgg
     9361 tccatcccag tacccagcct ccaatcagaa tatctgaaaa acgaccatca ccaatgactg
     9421 cgccaaatac agcaaccaga aggaatactg taaatatggt tatggcgatt ggcgaaatat
     9481 gttgatcttc tttagtttgc ttgttctttt caagtaaaga cttaaataga cttgtattca
     9541 tttgacattc tccttctgtt gttcaagcgt ttctttcatg gacttcaaat tgtctctcac
     9601 catttgactg atctctcgct ctgagaatcc cagccgaatg gcttcgctga taaatcgttg
     9661 tgtcaacgtt gccagcgact cactccgcaa cttttcactt acctttggcg gtggtatctc
     9721 ggtaatatac gttcctcttc cttgctgtgt ggatataact ctggcttcat ccagtatgcg
     9781 ataagcacgc gctacagtgt tgaaattgac gcgtaattcc tgagctaatg ctcgaacggt
     9841 aggcagttga tctccaggca tcaatattcc attggctagc tggctttgta tctgattgac
     9901 gatttgggtg taaatgggca agcctgaatg gaaatcaaga cgcagggtga gtttcttttc
     9961 tgtcattcgg ttatcctaat tgtactaatt gtatcattgt aataattgta actatttttg
    10021 agtttaagtc aagcccttcc cttttaatgg aatccagtgg aataccatgg atctgggaat
    10081 gtttactttg atgtccagcg aagatggttc atgccactga atgtggtaaa ggttggaatt
    10141 ggtcacaaca tgtccctttc aatgtttatg ccgcaggcaa tacattactt gaatttatat
    10201 tttcacgagt ccagggattg gaaaatatgt ttacagtgct cttatgattt aaatatggct
    10261 gcatggtaga attcatacgc attaattaaa ggagtgtata aaaatggaat caaaaactgg
    10321 tacatggact gagaaaaagg ggctggccca aatgttaaaa ggcggcgtca tcatggatgt
    10381 ggtgaatgcc gagcaggcca agattgcaga agatgcgggc gcatgcgcag taatggcgtt
    10441 ggaacgtgtc cctgctgata ttcgtgcaga cggtggcgtt gcccgcatgt ctgacccgga
    10501 catgattttg aagatcatgg acgctgtaag catccctgtc atggcaaaat gccgtatcgg
    10561 tcattttgta gaagcacaga ttctggaatc gattggcatt gactacatcg atgaatctga
    10621 ggttcttacg cctgcagatg aagaacatca cattttgaag cataacttca aagttccgtt
    10681 tgtttgtgga tgccgtaata taggcgaggc tttgcgtcgt attggcgaag gcgcggctat
    10741 gatccgtacc aaaggcgaag ctggtacagg tgacgtggtt gaagcggttc gtcatgcccg
    10801 tagcgtgatc ggctccatca agcaattaca ggtgatgggc gatgaagaat tgatgacctt
    10861 tgcgaagaat aacggcgcac cgtatgaact ggtaaaggaa gtgaaagaac tcggacgcct
    10921 gcctgtggtt aattttgctg ccggtggagt agcaactcct gctgatgcgg ctttgatgat
    10981 gcagctcggt gtggatggtg tgtttgtagg ttctggtatc ttcaagagtg gtgaccctgc
    11041 caaacgggct gccgccattg ttaaatcagt gacacactat caagatgcat ccatccttgc
    11101 ggaagtgagc aagaatctcg gcgaagccat ggtgggacgc aacgccgctc aaatgcctga
    11161 aagcgaaaag ctcgctgttc gtggttggta aactattgta ggagcatggc gccgccatgc
    11221 tcctacggaa tttgaaaatg aaaatcggag ttctcgccct gcagggcgat tttgcagaac
    11281 atatttccat gctgaaaaag atcggtgtgg atacggctga agtacgcctg ccaaaacatc
    11341 tggatgggct cgatgggttg atcatgcccg gtggcgaatc gacaacattt ggaaagctgg
    11401 ctgttgccta tggtttgatt gaccccattc gacaatttgg aaaaagccat gccgtttggg
    11461 gcacctgtgc cggtgccatt tttctttcaa aagatattgg ccgtgaccag cctttgctcg
    11521 gattgatgga tatcaaggtc cagcgaaatg catttggcag gcaggtcgat tcctttgaaa
    11581 ccgaccttga gattgatgag ttgatgaaag ccactggcac agaacatcca tatcatgccg
    11641 tatttatacg ggctcccatc atcgaatcgg tacatgggaa tgcacgggtg ctttcggccc
    11701 tggaagatgg ccgcattgtt gcagctcagg aaggtcattt gttggctact tcctttcatc
    11761 ctgaattgac cgacgacaca cgttttcacg agtattttat ttctctggca tcgtaaattg
    11821 caggggcaca gtatttcact gtgcccctgc actataaaaa atgtccatcc gcccacttga
    11881 cctgcttgat ctgcctgtta ttacgcgata tcgaaataat gtcttgactc tcgatagcgc
    11941 gcgtgccctc acgcgtggac atccacttgg ggcgatgggg ctgctggctt atatcaaccc
    12001 tgcccgtcat ttatatgcgg caatcgcaaa tggcggagat acggcccttt tgggcggcgt
    12061 aattcacaca cgtggtgaaa catttgcaaa gctgttgtat ctcgcccctt cttccaattt
    12121 gggggaccca accctgcctg cgttaattga gcatttggcc gcccaggctg gcgattggaa
    12181 ggcatttcat gtattggctg aggtggatga atcaagtgat gttttcccgg cattgcggac
    12241 tgccgggttt tccgtctatg cctggcaaag actttgggat gtgagcctgc ttgagaaaac
    12301 agaatcagat atcagcttaa ggtggaggcg cactcaatcg ctggacctgc ccgcagtcca
    12361 aagtctgcat tatcagatcg tgccgccgtt gatgcatcct gtggaacctg ccccgaaacg
    12421 ggtgcttggg tttgtatgcc agaatttgaa atgttatgcc agtgtaactg cgggcatgta
    12481 tggaattgtg ctaaccccgc ttatccatcc tgaagagcaa aatgtcggcg agaaattatt
    12541 ggcattggtg gcagggctgc cagatcgccg tggccgcaaa gtttatttaa atgtgcgttc
    12601 gtatgaagcg tggcttgagc ctgtgctggc agatcttggc gcgcaggcgt catcgcgtca
    12661 ggcggtgatg gtgaaacatc tggcgcgatt gatcaaggaa ggccagtctg tgcgtacggt
    12721 tccaaccaat gtgggcgtgc ctgcttcgcg gattagtaga atggattttg atgagtaatt
    12781 gggcaaccta tgatggtggt cgcagcattg gcaaaacaag cccggctggc gatgtcatcc
    12841 tctatgacga agagcaccat cttggtgcgc gcatcacatt gaggcgcggc aagaatttta
    12901 tttccgtctc ctgtaatttt tacgggtgga tggatcacac acgtttcttc aatgcgcttt
    12961 ccgaggctga gcgtgaatac agagccatga aaacggcgat cgccgcgatg atggaaccct
    13021 tgaaagccgc cgcctcgaac aagctcaagg ggtgggaagc gatctcagaa tttgtaaggc
    13081 ggtttccata aaatgttgaa agctttgatc tttgattttg acggactgat tcttgatacc
    13141 gaaacccccg aggtattggt gtggcagtcc atttatcgtg accacggttt tgaattgcct
    13201 gtggatgaat gggaaaaaac aattggcgga tacggcctct caaactttga tgcggcggaa
    13261 catcttgcgc ttctttcttc gggaaggctg gattctgttt caatgcgcag ccgctaccga
    13321 aaagaagcag acgcgatcat ccatgccagc cccattttgc ccggggtggt tggtttgatc
    13381 gaacaagctc gtgaacaggg cttgcaggtt gccattggtt ccagttcgcc gcattcatgg
    13441 gtggatacac atgcgaaaag gctcggtatc tttcattatt tcaaatacat catttgtcag
    13501 gatgatgtag cccccggccg caccaaaccc aacccggata tttatttaaa ggcattggaa
    13561 gtgctcaagg ttcaaaatac cgaagcagta gtttttgaag attctccaaa tggtgtcctc
    13621 gccgcgcggc gggcaggcat ttttgcagtg gctgttccaa acccgctgac aaaacgactc
    13681 ggtgtttccg gcgacctgac cgttccgtcg ctggctggga tatccctgca tgaaatttcg
    13741 caagcgatct cattgagatc ctgaagtcaa agaccatgtc gtcatttcag aaaaagatcg
    13801 ggttgatcgc cgtgcttgca tttgcattgg ctgggttgtt gtttttcagg cccagccgta
    13861 caaccccgcc tgctgactct cccactgtga tggttgttga tgtgaccgca acctccacct
    13921 ggaccccgtc gcgcattcca gaaaatatcc ccattgcaga tgacccattg aaagagtcca
    13981 ttcttgcggc gggagattac cttgtgcgcc agcaattgac caatggcgag ttgtcgtatc
    14041 aggtggattt tatttcaggg gaacggtctt tttctccctc acatgttcgt ctgatttcgg
    14101 gaacaggggc gctttttact gtgtgcaggg tttcaggcga ttcgaaatac tgcgatgccg
    14161 ccgaccttgc gctcgaccat tatcttgagt tgctggtaac cgatcccgca catttcaaag
    14221 gaacctgttt attcacggaa ggcacctgtc aactgggcgg cgcagccatg accgtggatg
    14281 ccatttataa acgctggcag gccagcggcg aactcatttt gcacgagcgc aatttgcttg
    14341 aaaccgcgat caaccttggg tatttcatcg tttccatgcg cagaccggaa ggcgggtttt
    14401 accatgcata tgacccatat tttggcggca ttgtggatga tgatttttac ctgtcctatt
    14461 ttcccgggca gagtctggtt gcgttgaccc agctttatga gatcacgggg aatgattttt
    14521 ggttggagca ggcccgtgag gtgaacgatt tcatgatcac acagcccatc tctgaagacc
    14581 actggcacag ttacggtcta tccatgctgg ctcgttggga tgcgctgact gaagccgacc
    14641 tgacgtatgc caaacagatc gctgatgtgg tcattgccgg ggaagtgcgt tcacttaatt
    14701 tgaagaacac cagtatggcc accgcaacaa agatcgaagc actttcggct ctggcgcagg
    14761 ctttttatct ttcaaacaca gaccatgcga agctggagcc tgagatccgc gcctttatca
    14821 catttgtgca ggcccgtcag cttccagaca ataattgtgg ctggaccatc tcagaagaaa
    14881 taaccgaaaa atttggcggg ggatttttta gcaattgtga agataccacc atccgtgttg
    14941 atgggcagca aaactggatc aacggggtta cggcatatct tgaatatcgg agtatgatta
    15001 aagccaggaa ataataaagg gagcaaagca gagttcctgc tttgctcctg ctaattcggc
    15061 agacctgaaa aataatctta tgaaagaagg agattcccat gagtattgga aaaataaagt
    15121 ggcaatccat cgtttcgcaa tatgcatatc ctgaaacctg gcgtagtttg tggcaggtgg
    15181 tcaattcggt tattcccttt ttcgtgttgt ggtatctggc atatcgcagc cttgaaattg
    15241 gatattggct aaccttgttg ctcacaatcc ctgccgccgg gttcatggtg cggatgttca
    15301 tcatcttcca cgattgctgc cacggctcgt tctttgaaac gccgcttgca aacaaccgtt
    15361 tggggctgct gcttggtgtt gccgtgtgga ccccttacta tgcctggaaa cattcccatg
    15421 ccattcacca cgccaccgcc ggtgaccttg accgccgtgg gataggcgac gtctacacca
    15481 tgaccgtgga agagtacctt gcctcgccct ggtacaagaa attcgggtat cgggtgatgc
    15541 gtaatccgtt cattttgttt accatcggcg cgttgatcgt ttttgtgttt gcacatcgtt
    15601 tctgggaaaa aggtgcaggc aagcgtgaaa agaacagcgt cgtttggacg aacattgccc
    15661 tgctcggttt tgtcggctgg acgatctacc atatcggctg gcaggcttac ttgctggttg
    15721 aaattcccat tttgctgatg gcctgtggcg ccggggtctg gttattctat gttcagcata
    15781 actttgagcc gacctactgg gagcgccacc agaattggga gttcttcaag gcgggcatgg
    15841 acggcagttc gttctataaa cttcccaaag tgttgcaatg gtttaccggc aatattggtt
    15901 tccatcatat tcaccacctc agcccgaaga tccccaatta caaattggag aaatgtcaca
    15961 acgagaaccc gatcttccaa attcaaccgc ttacgtttag agacagcttg aaatcgctgt
    16021 acttccgttt gtgggatgaa aaggaaaaaa tgctcgtcgg ctggcgcgcg atcaaaaaat
    16081 ataaaatgaa aatgcagatg gataattgat tcccgagctt gtctcagagt tgggagaagc
    16141 ccataaatga aatatgaaac accccgaatt tagtctttca attctttagc cgcggatttc
    16201 gcgaatttac acgaaaaaag ccgatccatt acagggtcgg cttttttata aatcgtctgt
    16261 gtcttgtaac tcatttgcct acttgcataa ttactcctct ttcaattcga tcagccgttg
    16321 ttgcagttgc gtttggactt ccgcatattc aggctgaccc gccagattgt tcaactcgta
    16381 cgggtcattc accaggtcgt agagttcctt ttcacctgtg gaatattcca cgtatttcca
    16441 ttcggcattg cgcactgcat aaaattcggg gatatccgca ccgactccat cttctgtggg
    16501 ccagtgttcg aagagcaggt catcgcgcca ggtgatgctt tcatttttta gcaaaggaag
    16561 caggctggtg ccatctactg tatcgggcgc ggcaaagcct gccagatcgg cgaaggtggg
    16621 ggcaaggtca atattggcga caaaacgtga ctcggtgcgt gcagggaaca tgcccggtgc
    16681 gtacatgatg aacggcacac ggatgcagct ttcgtatgcg caattctttg tcaaaccgaa
    16741 gccatgatct cccacggtca atccattgtc tgaaagataa acgatgatgg ttttctcgct
    16801 cagccctgtt ttatcgagtg cattcaaaat ggatgcgaca ccatcatcca ccgacagcag
    16861 ggaacgcagg atctgtttgt aggtgatgtc tattttgctg actgcgatcg aattcaaatc
    16921 ctgcaaatac tttggcttgt cactgcggtc ttcctccatg aatgcagggt tgcgatacgg
    16981 ctccgccttc aaagccgagt ttgcgcggaa ttgctcgtcg tgccgctctg cccaaaaata
    17041 cggcgagtgc gggttgaaat atcccaccac catgaagaat ggctcgtctt ttgaagccgt
    17101 gatgaaatca accgcgttgc gcgtggtcac atcggcacaa aattcttccg gggtttcgcc
    17161 ataggctttc acggttccat tgactgaaag attgtaatta taaaaagccg cgtcaagcag
    17221 agttccccat tcatcccagc ccggcggtac atagccgtag ggcttgaggt cttcatacga
    17281 gtttagatat tttccaaaat acccggttcg atagcctgca tcgttcatcc aggttgcgat
    17341 ggtggaagag tcatccagtt tgggcgcgcc gccgagcggc atgcggttgg tcaacacctg
    17401 atggttgtgc gcatattgcc ccgtcaacat gctcgaccgg cttgggcagc acaatggcgt
    17461 ggtgacaaat ccattttcaa aattcacacc gttcttcaaa agcacatcgc gcacggtcgg
    17521 catgtattga atggtttcat acggctggtc atcggtcagc acgaagatga tgttcggacg
    17581 ttcgtctgca agccgcgatg gagaagcctt cgcatctgac ttaacctcga aaatggtcgc
    17641 tgaataaggg gcagggtcag caattggagc ggatgtttcc gtcggcgccg gggttggttg
    17701 tgcgcaggct gcgaggatga ataagcccgc aaggaatgcg gatttcaatc tactttgtgg
    17761 ggtgcgtttg ttttttttca tttttagtca gggtactgtt gcagggagag aatgatcggg
    17821 aggcgaagca ggcctcggca aagcgtgaag cgcgagtcat tatatcgtaa taaaaaacag
    17881 cgcagactgc acttgtctgc gctgttttgc atgagtgtgt cgatttacac gtatttttcg
    17941 cgcaacacag atgaaatgta atccatgctt gctacaacaa tggcgatggc gagcatttgg
    18001 gtgctggcgg cgcggtagtt aagcaggttg atattttgct gaagcaggaa gccgatgccg
    18061 ccgccgccca cgaaaccgat gatggtggac atacgcacgt tgatatccca gcgatacatg
    18121 gtgtaggaaa tgtatggcgg gacgatctgc ggaatgactg catagataat ggtttgcaga
    18181 cgattcgcac ctgtggctgt aatggcttcg agcggaccag gcatgatgct ttcgacctgt
    18241 tccgaatata gttttgcaag gctgacgatg gtatgcaggc cgagtgccat catgcctgcg
    18301 aacggaccaa tgccgacggc aatgacgaat acgatcgcca tgacgagggc ttccacagag
    18361 cgcaatgcat tgaggatggt gcgggtgatg ctgtaaatga ccatgccggt gggcagggtg
    18421 tctttgggtt tggtgaagaa ggcaagcaac aagccaagca gtgcgccgat gatgatcggc
    18481 ccccaaaaag ttaattcaac acggccgatt tgatacagcc attccaccag ccagccgctc
    18541 aaaccaaaaa tggttgcacc tgccagcgcg gctacgatga aattgatgct cttcacacgt
    18601 gcagcggggc ttttctcggc agtgtgctgc ccgatgcggc ccaggaagtt ggaaagcgcg
    18661 ccgcctgctg ccaatgcacc caaagctggg attgaaatgc gcacgatgtc actgacctga
    18721 aaaaggaagg tagccagaaa accaagcacg ccaagcgatt cttcgatggc aaggctgaga
    18781 ctggttgtga acccagccag ttggaaaaga ccaaagaagg ccaccagaat cgcaaggaag
    18841 ataaccagca tacgggtcag ccgcatggca agttcgggaa tttttgtttc ttcctgcggc
    18901 aaggcccaac gcattgcata ccaaaaaatg aaaggcgttg ccacgacact gatcaaactg
    18961 atcgcgatgt tttccgaaag tggaatggca agcttgccca caagattgac gatgaaatat
    19021 cccagcccca tgccaatggg ccagcccagc acggaaagag aaacactggt gagcggactg
    19081 cgcacagatt tcatcaggtt gcgggcagcc agaaaactga tggggaaggc aaggatggtg
    19141 ccagcggtcg tggccagcaa ggccatgaac accgtttcaa tgatcttatc ccaggtgtct
    19201 tttgcgtttt gggtgaagtg tggtgcgccc acgttctgac tgatggtcac gcggatgaat
    19261 tgcggcgcat cgctaagacg ctcaggcatg acgaatgaag tggagaaatg tccggagccg
    19321 tcggtttcaa ctgtttcgcg gccgagttcc accacgttgg cgggatcatt gcccggcacg
    19381 aatcgaatcg gaccttcggt atgggcaaca aaattgaagc cttccacctg cacagattca
    19441 ccgggtgccg cgcatgccgg gctgaccgta aggtatgcac cggatgtatc tgcttcgggc
    19501 agggtggcca gcaggtcatc tgacgggcag ggaacaaaca ccggcaggtt gacaatggcc
    19561 ttttcctggt catagccgat gatatcgggt tgagccagtg tacgggtgac gcgggtgagg
    19621 ctttcctgcc tgcgttcact gcgcaattct tcaaggttga ccttggtgac atcgaagccg
    19681 taggcgtaaa cgaaaagtcc aagcaaaacc gccagcccga tttgaaccga tctccatgca
    19741 gatgcgggtg aatttttttc tttcatgggt tatcccaccc tttctgcatc gcggccatag
    19801 atctctttga acttcatgtc attgatgtct ctggggtgtc cttcgaatac cagttgaccc
    19861 tggttgagcg caatgacacg gtcagcatag cgatgaacga gatcgaggaa gtgcaggctg
    19921 catagcacgg ttacaccatc ttttttgttg atctcttcaa ggtgtttcat gatgctgtgc
    19981 gcgaggaccg ggtcaaggct ggcaaccggt tcatcggcga ggatcatgtt cgggtcctgc
    20041 atgagcgcgc gtgcgattcc cacacgctgc tgctggccgc cgctgagttc gtcggcgcgt
    20101 tggtcggcct tatctgcaat gcccacccgc tccagttgtt tgatggcttt ttcgatgtct
    20161 tcttttggaa agcggttgag gaaactccag gctgggttga cgtagcccaa ccgtccggcc
    20221 agcacattgg tgatgacctt cgagcgatgc acgaggttga aatgctgaaa gaccatgccg
    20281 atcttgcggc gaatgaggcg catttcttcc ggggtggcgg cggtgacatc ttcaccatcc
    20341 cataaaatct ggccttcggt ggggtctatc aaacgattga tgcagcgcaa cagtgtggat
    20401 ttgccggagc cgctcaaccc gatcacggcc aaaaactggc ccttgggtac ttcaaaagag
    20461 acattgtcca gcgctttgac gcccccgtcg tatattttgg ttaggttttt tacctggagc
    20521 attgtggctt ctcacagtct tttgaaaaga tgtttgtgct tgatgcgttt caccagatta
    20581 taagatataa gctgaaaata acagctcaaa taaaaacttg gatcacaatt gctgtacggg
    20641 tatataaaac aagttcgggc agggaatggc tccctgcccg aaaatttatg aaaagcggat
    20701 cttacttggg gggttcgtag ccggtggcgg caaccatggc gcggaccatg tcatactcgg
    20761 catcggtggc cggggcaatg ccagaccagc cgtagaaatc tgcggaacca atggaggtgc
    20821 cccaggcttc ggttgcggcg aaggcgagca gagcttcttc gatctgggcg cgcaggtcag
    20881 cggggaattc ggggccgaag gacagggtgt cgttcgggat cgcggcagag acgccgagaa
    20941 tgcgaacctt ctggatcacg tccggggctt cggtgcggat gttggcacgg gcatcaagaa
    21001 tgcgccagcc gtcgcaaagc aatttcttgc cttcggtatc cggagcacag ttgggaacgg
    21061 attcagtggg gatctcatac tgctcgaggc tggtgatctt gccagcgacg aaatcatcgt
    21121 agctgaagag tggagcgcct tcaggggtca gcggaacgct gtagaagacg gtaccaaaat
    21181 caacttcgcc gttgtacaca gcggtgacgg tctggttgtg accgccggtc tgaacctgtt
    21241 cgccgggggt gatgccagcg gcggcgagtt caacggtggg gaccatgtaa ccagaggttg
    21301 aaccctggtc accgtaaccc cacttggcgc cttcgaggtc ggccagagtc tggtatttgc
    21361 tatcgcgggc tacaatgtat tcagcccagt agaccgggaa gccgaagcgg atggccttga
    21421 aggaaacatc cacgccgcac aactggctgg caatggcata gccaaggcca gggatgaagg
    21481 ccatggtatc tgacggggag gcgcacattt cttcgatggt tgcggcatag gaggtcggga
    21541 cgaccacttc aaaggtgagg ccggtcgctt cgttcaaagc agcagccatc acttcaccgc
    21601 cggtcacaat gatgttcgca tctacagagg ggacgaacaa cactttgatg gggtgttcag
    21661 gggagccaat ggcgggcatg ggcggttcag tagcgacagg agcctcggtg ggggcttcgg
    21721 taggagcctc agtaggggct tcggtggcaa cgggggcttc agtggcggca gggccacaag
    21781 ctgacagcat cattgatatg gcaacgacga gcgtcagcag gacaaaagcg atacgtttgt
    21841 tcattatctt ctcctcttaa gagataaaaa gtttatgggg cactataccc ctgacttaaa
    21901 gataatagtt cataacggtt gtacaaacaa gtacgcttgt cttaaaaaaa gattatgttt
    21961 ttcactagtt gcttttctgt atttttcgct tcacaaagtg cggatgaaga actttaattc
    22021 ctctgtatcg tttggaagtt tgatcatttt ctcgaattta agtcccagtt tttcgagcag
    22081 accgatcgat ttttcattat ctggggtggt gatgcctgcg attcgcttca aacccagcgt
    22141 gtttttgctg tattccatga ccgcagacgc ggattcaaat ccatacccct ttgattcgtg
    22201 ttgggcgagg aaggcaaagc cgatatcaat gtcatccaat ccatctcttt ttacaagtcc
    22261 acacaggccg atgggaagat tctcttcctt caagagggtg atatacaacc caaagccatt
    22321 gtctttgtaa cttttaacca gcctggtttc aatatattca cgcgcaccat caaggctgcg
    22381 gatgccccgg tccccgatga aacggatgaa tgagggctgg tttaacagat caagcatgaa
    22441 aggcgcatcg tctgcgtttg cattgcggag aattaagcgg ggagtttcga gaatgttcat
    22501 aaagataaag ccttttttca tgaaatttgt gtggtggttt caactataca actaatccaa
    22561 atgtttgaca acaaaaaagc cattccgctg acaagacgga tggccttttt gttgttattt
    22621 ttaattccag tgcttacttt gtaatgcttc cgctcaccac actgttgatg ctgtcaaaac
    22681 tatagcctgt tttgcggata ttggtaacag tcagtgtgta ggtgccgcga ttgccactgg
    22741 tgctgaattt ggcgacaccg tttgcatttg tttttgcatt ttgtgtgacc acccgcccat
    22801 ttggcttgac ccaggttatt gagacgatag caccgggaat aactatgcca ttctcgtttt
    22861 gaactataac ggttccactc acattgaccg tggtcttttt caaagtagct gaaaggttga
    22921 ccgccgtact tcgcaccaca ttgctgagca caggattcac gatgatcgtg gtcgtggcgc
    22981 tggcactggt tgcgttattg taggttactg tcagtgtggc tgtatatgtg cctggagtgt
    23041 tgtaggtgtg gcttccatta ttgataaatg atggggcgcc atcgaggaag aaataaaggt
    23101 cacttgtgcc gtctccataa tccactttgt agttgttgat gaggggacca ttcggattca
    23161 tagtggcaga gctggtgagg ttgacgacca atggcgcagt tccactggca ggggaggcaa
    23221 caattagggg aagctgttcc ggggttacac tggggtcgac agtgatgatc agcgtggtgg
    23281 taatgctggt ggaattgttg tagaacactg tcataacagc ggtgtaagta ccaggcaggt
    23341 tgtataccat gcttgaagtt ttatcaagga tgaaggttga tgtaccgtca ccaaagtcaa
    23401 tttcagcgcc caggccgggg cctgcaccga aatccaacat gacgtagctg acaagtgggc
    23461 cgttcggatc cgtggcgacg tatcttgaaa agttaacggt gaggggtgcc ggtccgctgg
    23521 cgggggtggc agtgagggtg ggttgatagt ttattgtttg aggagatggg gctgcaaaaa
    23581 ccggacgggc tgaaaatggc tgtatcacca gcgccaggat ataggccgcc agaagcaggc
    23641 tgcgaaggaa aatgtgtttt ggggttgtgt acataaaatt gtgtctcctt ggtgaaatat
    23701 ttttactttg tgttacccgc cgtcgcttct actgccagaa attaagaatg gacacaactt
    23761 taatgttttt ggcggcatgc ctgcatggat atcttattta tttctagtcg ttttaaaccc
    23821 tgaaaagtta cgggttaaac tgcctcaata aggaaaaaaa gcccctgcgt cacaggggct
    23881 taatctaacc actggttatg ctttttctcc gtacaactta cgatgcacga tgcttaacgc
    23941 ccgcacctgt tctgctgcat gatatgaaga ccgcaccagc ggattcgact cgacccactt
    24001 gaaaccgatc tccatgccga aggctttgag ttcggcgaat tcttccatcg tgtaataacg
    24061 gtccatgggc atgtgtttct tgcttggctg caaatactgg ccgatggtga gaatatccac
    24121 gccccaactg cgttgatcgc gcatcacctg cttcacttcg tccatggttt caccgaggcc
    24181 gagcatgatg ccgcttttgg tcagcacatc ggggtcgagt ttcttggcgt tcgataaagt
    24241 tgccgcagcc cactcataat tatcctgcgg ctgcacggtc ttgaacaaac gcggaacggt
    24301 ttccacattg tgattcaaaa tttcagggcg ggcatccatg acgatcttca acgcttcgat
    24361 gctccctttg aaatcgggga tcaacacttc gatggaacag ccgggcagca cctcacgaat
    24421 tctgcgaatg accatcgcaa agatcggcgc accgccatcg cggcgttcat cgcggttcac
    24481 cgaggtgatc accgcatgtt tcaggttcat tgatttcacg gcctgcgcta cacgttcggc
    24541 ctccccccaa tccatcaaca acggcttgcc atgttttaca tcgcaaaacg cacaggtgcg
    24601 tgtgcataca tcgcccagca tcaaaaatgt agccgtgccg ctgccccagc attcgccgag
    24661 gttcgggcac atggcttctt cacaaacggt gtgcaattct ttcgagcgca tcagcacttg
    24721 caggcgttca aaggtttcgc ccgaaggcgc gcgcaccttg atccattcgg ggcggcgcaa
    24781 tgggcgtgct tctgtttcgg gtttgactct gtctctcgta ggtgtggcag gcataaggtc
    24841 ctcttgattc ggacacggca gtgccgtgtt tctatttctt tttcacgatt aatcttaatc
    24901 cgcgtacttc gacaacttcg accgggtcac ccttacgaat ggattctgac ccgggggctt
    24961 tttctgcgct ccatagttcg cttcccactt gaacctgccc cgcttcttct tcgaacgttt
    25021 tggctgttcc tgttttccca agcaatgact caaccccggt ctggatcgga ttatggcgcg
    25081 agcgcaatgc aaccagcacg atcgcaaaga acaatgcgcc gatgaaaacg cccacaccaa
    25141 tcacaagcgg aacagatacg cgttgaaatt gcggtgtacc cggcgaattg aacaacacca
    25201 gcgccccgac gatgaaggat gccacaccgg ccgtggtcaa cgcgccgtgt gcggcggcct
    25261 tgatatcaag gatgaacagc acaaaggcta tgaccaaaaa gataaaaccg aaccagttga
    25321 ccgtgagtac acccattcca tacacagcca gcgtaagaca gactgcacca aggaagcctg
    25381 cgaaccagcc gcccgggctg gaaatttcga tcagaattcc ctgtacacca atggcaagca
    25441 ggataaaggc aatgttcggg tcggtgagca tcattaatag ctgttcgatg aaactgatgc
    25501 caagttcttc cgtgcggatg ttttcagtgt tgagcgtgac ctcactgcca tcgtccattt
    25561 tgacggtgaa accattcaag gcttgcagca ggtcttccgt gtcgtcggaa acgaaatcga
    25621 tcaattttgc ttcaagcgct tcactggctg tgacagcttt ggcatcatcg atcatggctt
    25681 ctgcgaggcg ggtggcttct tctccgcgtg cttccaccag aacacgggcc ttggccttca
    25741 gtatttcctt gatctttgtt tccagcgtgg attcaatgtc tgcaccggaa ctatcaatgg
    25801 ggctggctgc gccaatggct gtcttggggg ccatggcaga ggcatgcccc gccatggtga
    25861 tcaatgcccc tgcgctgcct gccatggcat cgtttggcga aacatacaca acgatgggta
    25921 ccttgctggc gcgcatggtt tgaatgatat ccagcatggt ttcaatgctt ccgccggggg
    25981 tattcagttg aatgacgagt acgtctgcat tgctgttttc ggcagtctca atgccgcgct
    26041 tgaagtattc caacatgggc ggcatgatgg tcccttcggc ggtcatcact acggccaatg
    26101 ggtcatcacc ctgtgcggct gcaagctgaa atgccgacaa cgcgatcatg agggtgaaga
    26161 ggaaaattcg aatgagtttc atttttgtat tcactttcca gtgtcattat aaaggatgat
    26221 gcagggaatt aagcagcaaa gggcgtaaag agaaacctct ttacgccctt tgctttttta
    26281 ttttttcagt tcctacggac caatgcgttt gttttccttc ggctgggtga gcgggatctg
    26341 taaatagaaa gtgctgccct tacccaccac gctatccacc cagaccttgc cgccgtgatt
    26401 ggtggcaatg gaatggacaa tggccagccc cagcccggat ccatgttgag cgcgcgcttc
    26461 acgctgcttg ccgcggtaga atttttcgaa caggcgcggc aggtcgtcgg cggcaatgcc
    26521 gatgcctgaa tcttcgacaa tgaaggtcag gctgcccggc tgcgtttcgg tgcgaatggt
    26581 gacacgttgg tcattgggtg tgtatttgat ggcgttttcc acaaggttat acacagcctg
    26641 atgcagaagg gcctgatccg cttcgatggc gtgcggcata tctttggaaa gttccacggt
    26701 caactggata ttcttttgac tggcttgtag ctgaagcgca ccggtcacac gttcgataat
    26761 atcgagcacg ctgacatttt ccacttgcaa gccaacccca aggtcaatgc gcccgaggtc
    26821 gagcagattg ttgacaaggc gcgacatgtt ctccacgccg gaaatgatct ttttgacata
    26881 tccctgctgt tgttcattca attcaccgac catttcgagc atggtggcat acccgcgcat
    26941 cagcgtaagc ggggaacgca ggtcatggct taccgtggcg acgaactccg acttcatcat
    27001 atccagttcc ttgaagtggg tcacatcgcg catgatgcac acacgcccga tctgtttgcc
    27061 ttcgaccatt acagccgatg cggtagccag ataggtgcgt ttgtcggcca gagtgatctc
    27121 agtggattga ttttctgtgg tcgcgctttc cagcagggca agcagcggtc tgatcttgac
    27181 gacctgttcg gtcttgcgac cgatatcact ggtggtgtct tcagattgtc ccagagctat
    27241 ggcggcggca cggttggtaa gcagcaaccg gttgcggtga tcggtcacca gcaccgggtc
    27301 cggtgtggaa ttgagaatgg ctttcaactg acggcgcgag gcttccacat tcaggaagag
    27361 gtgggcattg gcaactgcca gtgccgcctg accagcgagc gtggtcataa aacggatatc
    27421 tgaatcggag aacatgcgcg gttgttcaaa ccctgcccat accacaccgt agtaacggtt
    27481 ttcatggcgc aaggccaccg ccagcaggga gagcggctgc ggcagggatg ggtcgagttc
    27541 cagttcgcgg ctgcgagaaa ggtttggaag caccaccttc tcctggcttt gcgccagcgc
    27601 gaggatctgg tcatcaagat gggcgtaaat gtcctgcaca gtgcccacag caaaccgtga
    27661 gggcaattct acaaacgtgt cgggcaaaat acttggtgaa agcaccacac tgaccgagtt
    27721 tgcacccgtg gcaaggatgg cgtccaacac cggcttgacc gcatcctgca tttccaagct
    27781 ggaggcaaca ccctgactta cacggagcag gctgttgatt tcgttgaggc gtgcctgcaa
    27841 gctggaacgc atcttttcga aggcacgacg caactgcccc acctcatcca cgccttctgt
    27901 ttgaagcgga tggtcgaagt tgccctgagc aatgcggttg gcttcggtgg cgaggctttg
    27961 cagtgaccct gtcaccacac gcaagcccac ccgtaaagaa atcatcgcaa cgaccgccag
    28021 gacaaggatc atcaatgaaa gcggcatggc gatgtttaat gccaattgtt gggcctgttg
    28081 cgcgggcacg gttaacacaa ctgcccaggg gcggcccgtc accggttgat aatagaccag
    28141 ttgtcgggtt ccgtctgccg cggtgtcgtc gtagaaaacg gcttcagtgc tgcgctgacc
    28201 gttgtatgtg gcagccattt caatggtttc agagtgataa atgatgcggt tgctctcatc
    28261 gagcagaaat cctgcgccgt tgaggttttt catgttgtca atgccttcga tcaaaggcaa
    28321 ggtcaacgga ttcgttttca gggtggtgcg gccgatcaat acccgttgta ccgaaccgaa
    28381 cttatctaca ataccgacga ggaacgaaac ccgtgccgat tcatcagcgg agatcggtgg
    28441 gatggaatag atctgggtta agaccccgtt ggccgccaga atcaacccca tgctttcatc
    28501 gggatacaat ttgaaggtgg taacagagtc atccgggtac cctgccagca gggtttcact
    28561 attggcttcc agtacgaaga actggtcaaa atatggcacg gcctgaatac gcgagccaat
    28621 gatggaatgc aattcatcac cggtggcttc caacaggcgc ggatcggcag cgagctgcac
    28681 cgccaggttc tgtccggttt caaggaaaaa aggaatgctt tgggccgctg cttcacccgc
    28741 actggtaagg cggtcttgca acatgtcacg agcggcctga cccgccacca cccaatcacc
    28801 gatcagaagg gttaacaaca aaagggaaat aaacgtgcct actgcaaatt gaaaacgcga
    28861 ctcaaggctt ttctcgccag gcgagggctg caaggcctgc tgttttccac cccaaaccgc
    28921 aggaaaggac atggaaatga tctgtgccac aatgccgccc accagcattt caattccaaa
    28981 tgcgagggtc acaatacctg tgttgctgat ggcaaaatca agccgcgccg tggtggggat
    29041 atccacaccc cattgggtga acaatgccac cacaatgtag aacaacgcat gcagcggaat
    29101 caacaacaaa gcgccgacaa tgggttgtcg taacaggcta tacacaggtg tgcggaaacg
    29161 ctgacgggta ttcgtggaaa accacgctcc catcaacgca aattcaatga tggaaaaaag
    29221 cgaataggta tcaagtgtgc cgcgcaggag gccagccaaa gcgccgaccg ccgccgcacc
    29281 aagcggcccg atcaacccgc cgctcaacaa ccatggaatg gctgaaaaaa cgatcagcgc
    29341 cgagcccggc ggttgaaccg gcaagttggg taaagggcgt gctgaagaag caaaaggtct
    29401 taatataaaa acgtttgcaa caagtgtcag gagtaaaagg ccaactaaaa atccccattc
    29461 aattcgtttc attgcgggct ggtaggcacg ccaatggtac agggcataag ccagcattcc
    29521 cagcaaagac aaccatactg cccatcctgc aagagtggga ggggcgggga atgcgatgct
    29581 tgccggcagc gtggaaagtg atcccatttt ctgaattata gccccaaata attacaattt
    29641 taattacaag attgtggggg gcaccgccca atttccctaa aatcagcctg aatacgcgcc
    29701 gcggtatttt tctggaagca gccccgccag cgcctccatg atctgacgtg tcccctgcgc
    29761 gatgacctcc tgccgctccc cggccccgcc tctttctccg ccctgggcct gcccagccaa
    29821 cttgaacatt ttccccaccc gcagattcac ccgcgcacgg cggaatttct tcaaattccc
    29881 atacacggtc tcattttcag ttcctgttac agcaatcggc aaaaccgccg cccccgattt
    29941 gtaggccagg aatgccgcac cgttgttccc ctcttccaat gccccggtga gcgagtaccg
    30001 cccctcaggc gcaataacaa tcacccgccc ttccgccagc ccatccaatg cggcacgcag
    30061 ggcgcgttta tcggggcggc cgcgatgcag ccaaataaca ccataccagt ccattaattt
    30121 tccgaggatg gggaggtcgt acagctcgac tttggccaat gcatcaggcg gaaatggcag
    30181 tgacgagatc aacgccggga catctgagtc gccaagatgg ttgatgacca ccaaaacagg
    30241 ctctgctccc gccttgcgcg gaaaattctc caagccttcc actgttacat gcaggcagag
    30301 tttggccacc agttttgtca atccgtgccc aaagacccga aatgcgcggc gggcatgggt
    30361 catttttggc aaacgaacca actcaggccg ccatacttca ctgactggtt tgggagtcgg
    30421 ggtatccata attatgtaga aatggcctgc ccggtataaa ctccatgata ttccgcgggc
    30481 agcagcccgg cgacatgttg catcaccagg tctgcatttt tctggcgggc ttcgcggcgt
    30541 tccgcgccct tttcaaggat cggctgaaag tggatcggtt ttccgatgcg catttcgagg
    30601 cggggttttt ctccctgccc gcgccaatac tgttttgaac gctgccagaa atcatcggtt
    30661 gtccccacca gacccaccgg cacaaccggt accccggcgt gctcgatgat ataaccaacg
    30721 cccggcattg cccggcgcat ggcgggttca tgcgaacgcc cgccttcagg cgcaatgacc
    30781 agtggacggc cttgtttcag aattaagatg atcttttcaa gcagggtgcg atcatacgtg
    30841 ccgcgatgga cagggatgac gccatataac gagaggatgg tcccctgccc tttttttgca
    30901 aacacatctg atgcgccaat gacctccggt tcttccggcc agaaactaac cacgagcggc
    30961 gggtcaaaaa tcgaaatatg gttcattgcg atcacgtacg ggctgccaag cggaatattt
    31021 tccgcccctg tgatctttac actccccaaa atgttgaaca atgtgcgaat gccaaaccgc
    31081 aaccacaacc ggttggcctg aatgtgaaat ggaatgcggt agtcttttac ttgcataaat
    31141 tcaacacgta tgcaaaaacc tgttctgcgt ccattgcatc cgagtcgatg aggatggcat
    31201 catcggcggg gcggagagga gcaaccgcac gcgtggagtc gatgcgatcg cgttcgatca
    31261 cctttttgag aatttcatcg aggttggcag gttccccgcg ggcaatgatc tcgttgtatc
    31321 ggcggcgggc gcgttcttct gcactggcat cgagataaat tttgagatcg gcatttggca
    31381 ggacgaccgt gccaatatcc ctgccgacca tcaccacgtt gccgcgcatg ccgatgcggc
    31441 gttgttgctc cgataacgcc tgacgcactt tggcataggc ggaaaccaca gaaacattcg
    31501 catccacatc cccgccgcgc atatcccagg taacatcttt ttcaccaatg atcacatcgc
    31561 aggcacgtcc atcatttttg gagggcgggc ggatgtcgat ctgggcattc tgcgcaagtt
    31621 gagagaccgc ggcttcattg cgcaggtcca tgtcatgttg tagtgcgatc caggtgatgg
    31681 cgcgatacat gataccggta tcaaagaaca ggaacccgag tgcatcggcg agtctttttc
    31741 cgagggttga tttgcccgat gcggcagggc cgtcaagtgc aatgatggat ggagtgggtt
    31801 tatttgtcat ttttttccat tcatttaggg gatggattga ttggccttgt cgaggaaaag
    31861 atcatcacga gcccagagaa tgatggattc gccggtttgc ggaacttcgc gcagagtcac
    31921 ccaacggatc caatcctgtg gcagggtctc attccacagg aaggtttttc tccaggtgaa
    31981 atcctgcccc cgataggtgg aggcaaaggc ggggtcgttt tgaagcgggg taatcacaaa
    32041 atagggcgca ctggagatat caagcgtttc aacaacctcc acttgatgtt cgcgcaaggc
    32101 ccattcgaga gcgggagaat ccaacccggc gatgatcacg ggggccatat tgtcgttgcc
    32161 ggtgccccac tcagaaaggt cgttgacgct tgcttgaatc aacttggctt gaactggcag
    32221 cggcgcctgc caccagatct cagggttatc caaacctcgt aaacctgccg caccaaaagt
    32281 tccgcccagg ccgagtgcgc caaggaccag tgccatgccc aggaccccgc cgatacgcgc
    32341 cgtgcgaatt gaccaacccg ccgcaaccag cagaagactg atggcgatca ggagcagtgc
    32401 gcctaccaaa agccagaagc gcatcatata ttcgcgggtg gtattgggaa gccagaccat
    32461 gccggagaaa tcgagccagg caaataccca tatgaaagcc gtgaggaaaa ccacaccgaa
    32521 aatctcattg cgctcttccg ggaagatatg caggttgcgg atgagttcaa gtgcggccag
    32581 cccccacagc ggaatgagcg cccaggatat atcggcgatc tcgcgcgatg ggatgaaaat
    32641 agccagcagc aacgccacaa tgaaccacag actcagtgaa atgatgcggc ggcttccatg
    32701 ctgccatcca cggatgaggg caatgacagc aagcagaagc cccagcggtt gatacacggc
    32761 cagcgcgatc agaaaacgtg aggcgggaaa atcagaagcg attgtccagc gtttgaagaa
    32821 ttcgggaatg gatgaaaatg cggcgctgag cccattgggg atgagaaaga aaaacgtgcc
    32881 cgccacaatc aaagtgacga cgaacggcca aagcgcttct ttgacattaa ccgaggcctg
    32941 ctcttcggaa ccacgcaatt gcacgccccg atagatcgcc catgcaatgc caatgctgag
    33001 aagccccagc cagatggatg gtccgccaag cagggcaagg gcggcaaagc tgcccgcaag
    33061 tttcaagttg ttgttttcat aaaatccaat ggcaaaaagc aggaaggtga tcgcaaagat
    33121 cggactcgcc gcctgccgtg acagggcaac catgcccggg tcaagcgcaa tgaaaagggc
    33181 caggatcagg cccgggcggg gtttgatccg ttcggtgaag agaagcggaa tgaggacgag
    33241 gaggctgccg attaaggccg gggaaaatcg ggcgaggaaa ttcgtcccct cgccataaag
    33301 gaaaaataaa accgcagtgg aaaggatgta aaagggatgc gggctgagca aggggcgctg
    33361 cccctgggtg atttgcaatg cctgcagggc gggcgcggct tctgaatctg agagaggcat
    33421 ggcgccaagt tgaatggtgc ggactgacac cgcaatcaac agcaccagaa catacaacca
    33481 ggtttcatgt ttaaagtgtc ggtaaatcat gggcgcaatt gtaatccatc cagaactgtg
    33541 agtctggtgt cctttgttaa attgaggtgc ggtttttttg tgccactgct atttgttttt
    33601 tcgttttgcg ggcttgccag cgggcgggtc ttttttatcg aacatcttta gaaatgcggc
    33661 gaccacggaa gggtcgaaat gttttccgct ttgttcacgg atgtatttga gggctttttg
    33721 ttttgtccac gctttgcgat aggggcggtc aatggtgacg gcatcccata catcaatgat
    33781 ggcaaagatg cgtgccacaa gcggaatctg ttcccccttt aatccgcgtg gataaccgct
    33841 gccgtcccat ttttcgtggt ggcagtaggg gatatcgagc gatttttgca gatacttgat
    33901 gggttgcagc atgtctcgcg catagttggg gtgctggcgc atgaccttcc attcggcctc
    33961 ggtcagtttt tcctctttca gcaggatctt gtccgggatg cccattttgc cgatatcgtg
    34021 cagcaatgcg ccgcgccgaa tgtggagcat ttggtcatcg ctgatcccca tggcgttggc
    34081 aagggcaagg gtcatttcgg tgacacggcg tgtatggcct tcggtctctt catcgcgcag
    34141 gtccatggca tgtgaccagc cttcgatggt cgtgtcgtat gcgatgatca attcaaggtt
    34201 ggcgcgctgc aggtcatcga agagttgggt ctggtcgatg gtgatggcgg cctgcccggc
    34261 aagggtttcg aggaagccca gccaatcgga acttgcatgc agtggggtgc ggtgaaaaac
    34321 ttcgagtacc cctttgatct cacctttggc aatgagcggt acgccgtagt attccagaaa
    34381 accctctcca accaaaagtg aggcgcgtgt aaattcggtc cctgctttga ccaaatttga
    34441 tacatgcaaa actgtacgat tcagcgcgac ctgcccgccc tgtccttccc cgaggcgcag
    34501 gcggctttgt tcaatgttgc gcgtgcggaa cccctgcccg gctacatatt ccagtgtttg
    34561 caggtagggg ttgaaaagca aaatatctgc cgcatccaca cccaattgag atatggattg
    34621 ggagatgacc gtattcaaga tgaggcgcag gtcaaaggat gatgagatcg cacggtcaat
    34681 cgtatgcaaa gcttgcagtt gattcacacg ttgtgcggtt tgctcacgta aacgtgccga
    34741 atgaattgct ccggccgctt gtgatgcgaa caaggtaagc aggcgttcat cggattcggt
    34801 gaatacccgt gtcgagtcat tgatctcgtc ggcggtcagc acaccgatca gctcgccgcc
    34861 atacagcatt ggcacttcaa gcaccgcacg gatgcgaagt tcatcatatt tggaggaacg
    34921 tccctcccat tcagaatagt tctcgattcg cagcggttgg tgggtttgtg ccacgattcc
    34981 cgccacacct tctccaagcc gcaggcggac gcccgtgaga atatcatttg tagtgatgac
    35041 cgacagttcc aattcctcat cttcagggtg gtaaaggtag attccgcttg agttggagtt
    35101 taataatttt ttggcgctct ccacaatgtt ttgtaacaga gagtccaatt cattttcagc
    35161 ggaaaggaaa ctgctggttt gataaagcga agcaaattcc agtgcccgtt gggcggtttc
    35221 gtcgaacagg cgtgcatttt gcagcgccag agaggcttgt ccggtaaagg cctgcagcag
    35281 ttctgcatgt tcttctgtaa agaagttcgt ttctgtgcta tcgagtgaaa acaaggcgat
    35341 tacctggccg ttgatgatga tcggcgcacc gagccacgaa cggatgaatt cggactctgg
    35401 aatgcgtacc cagccggggt attgccttac gtctgaaatg acaagcggtt gtttgttatc
    35461 gagcatccaa cgcaggtttt ccgcggtgga caggtcaaaa ctatagcttg atattttttc
    35521 gatcatttcc ttctttaaca ttttgtagcc atgcatcttt gtaatgcgga ccctgcgttg
    35581 ttcatgatcg acaaacatca tggtgccgcc cgcaaaaggc acgatgcgtt tgaccgtttc
    35641 cagcaaacgg tccaggatgg tttcgaaatc caggctggtg ctcatcaagg cgcccgcatg
    35701 caaagcgtcg cgcagtgcat cagacattcg gtgctgtttt ctttccgctt catacaaacg
    35761 ggcgttttcg atcgcactgg ctgcctgatt ggccagtgtc gctgtcaggc gttcatctgc
    35821 ctcactaaat gcgttgggaa gttcgctttc aatgctgata acaccgatga tgcgttcacc
    35881 caatttcatg gggacataga gaccggaatg caggccggga tatgtgtcca cgtaacgcgg
    35941 gtcattgctg agttcatcca cacgcactac ttgttgatgt tcgatggccc aaccgctcaa
    36001 gccctgaccg atatgctgga tcagcgcctg gaacctttct gccacctttt ccttttccac
    36061 ttcgtctgtc aaccctgctt gtttgaatgc cagcagttca aggccatcat cctgcggacg
    36121 tgtcaggcgg atggtgatat gatgccagcc caatttctgt tcgagcaatt cgacgatctt
    36181 tgtgcccaat tcctttggat tcaaaaggtg attgagcgca agtccgcttt catagaggag
    36241 ttccaactcc accagccttt gacgggtttc agcgaacagg ctggcgtttt caatggtggt
    36301 tgtggcctgt gctgccaggg tctcaagcag cacacggtga cgttcgccat acacattcgg
    36361 ttgatatgac tgcacagaga tcatgccaac cgttttacga tgtttccgca aaggcactgc
    36421 cagaatggag cgcacctcgt tgggcgtgcc gaaacggatc aatggtgtgc gtgcttcaaa
    36481 attgtcgtga atgaaaaggg tttgtccgct ggaaattacc tgtccgctta gcccctgtcc
    36541 acgcgggaca cgccgcggtg gaaatcgttc ctcaagatcg tataaataca cggcatggta
    36601 atcgccattc cccttgtcat ccagaacgat cgtgaaaaca tcacaaggca tgatctgaac
    36661 tacagcctca tgcaaggtgg agtagatcat gtctgaatcc aggtgtatcg aaagcaggga
    36721 ctgactgacc tgatgcaggg tattctgctc agccagccat tggttcacga tcttttccgt
    36781 gcgtttgcgc tgggaaatat cctgtttgat cgaaataaaa tgagtgatct tgccgttgtc
    36841 gtctttaagc ggggtgatgg tctgttcttc gtcatacagg ctgccatctt tgcggcggtt
    36901 gatcacttca ccatgccaga ccttcccgga aaggatggtg ctccacatgg ttttatagaa
    36961 acccggttca tgttcgccag atttgatcaa gtcacgaggg ttcttgttaa gggcttcttc
    37021 aaatgtgtag ccggtcaaag tgctaaaggc cgggttgacc cattcgatca ttccattggt
    37081 atcggtgatg acaatggcat tggccgcagc atttaatgcc gagatgcgca gataaagctg
    37141 gtcgtattgc tttgtaagat cttttatcac tgccaacacg gcgttttgac cgccgtattg
    37201 gattgtccgc agggtaactt ccacatgaat gacctgccca ttctttttgc ggtgttgaaa
    37261 ctcaccagtc agcgattcag aatcgactgg catttccgtg acagcataga tctctttgag
    37321 cgtaattgaa aggaattcct catggggata accatataat tgctgggccg cttcgttgac
    37381 gtcgagaagc gccagcgttt tcgaatcatg gatccacatg ggggtggggt gattctcgaa
    37441 cagcaggtta aaggcatctt tgtcagagaa ggggtctggc ttttgtgatg gctcttgcag
    37501 gctcatcaat tttctccatc cagagaggca gtgtagatca tggcacggtt tcgcccggcc
    37561 tgcttgacag catacaatgc cttatctgca cgctgaacaa tgaactccac actttcatcc
    37621 gcaggttcgt actgtatctc agcaatgcca atgctgagtg tcaccgagac catccctttg
    37681 tccgatggca tctgcatgtc cgccacgctg ttgcgaatcc gttctgcgat cgggtaggcc
    37741 tgttttgcgc ttgtttgtgg cagcaggatg atgaattcat cgcctccgta gcgggccagc
    37801 acatcgacgg aacgcatctg cgctgctgtg gcttgagcga tcaacgcgag gatcttatcc
    37861 ccggcagaat ggcccagagt gtcattgatg cgtttgaaat catctgtgtc aaacatgaga
    37921 atggacagcg gacggcgata gcgcatggca gcattgaatt cgcgggtggc aagctcaaag
    37981 aaataacggt agttgtacaa gccggtcaac ccatcagtgc gggcaagttg ctgctcccgt
    38041 tggagcaccc gttcgagttc ctgattggtt cgttcgaggg attccttggc gcgatgaagc
    38101 gcctcttcgg catgtttgcg ggcggtgaca tcacgcaggg taccctcgtg gtaaatgaca
    38161 ttcccgtgat cgtcgtaggt gtagctgccg cggtcttcca cccagatctc tgtgccatcc
    38221 ttgcgcctca gcctgtagat atccgtttcc ccatcaccgg tatccagttt gtgggtgcct
    38281 ctttcctctg catcgaaata gagttcggtc ttgatatcga tcgcgagcat ttcctcttta
    38341 ctggaataac cgaacatctt caccatggcc gggtttacat ctacaaattt gccttcatgt
    38401 gtgctgcggt agatgccgtc catcatgcgg tcgaacaaag aacggtagcg cccttctgat
    38461 tcctgaagag ccttgttcac cattttccgt tcggtaatgt cacgcaccac ccacagggtg
    38521 ttgttctcat ccatcggcga tatggttgca gcgaaccaga tcgttccata ttcaagtgat
    38581 atttcatatt caatgtgtgt ggattggcgg gtttccaaaa cctggcggat ggatttaaga
    38641 aagaattctg cacgctcggc tgggaagaac tccgaaagat ttttacccag caactcgctt
    38701 tttggtttga tcaatagttc tgagttggtt ggcgccactt tgcggtacac accgtcatgg
    38761 tcgatgacca gcaccacatc ctgcatggag gcgaacaggg cgcgcaattc aagttctgat
    38821 tcactttgat tctccacggc ttgtgttggc atggcaggct ttaccatgat caatgcagca
    38881 gcgcgcccat caaactcgat catgtgggtt tcggcttcca cctccacaga ttccatgtct
    38941 ttgcgtttgt gttgtaattt tttgtaggag tgaccatctt gctggacttt tcccccatcc
    39001 gctgaaatac gtgttactgc atcggcctgt aaaacatcgt ggaggttgag ctttcgaaaa
    39061 tcttcacgcg aatacagata ggaattgaca gcagcttcat tggcctccag aatttcgtag
    39121 gtctgtgcat cgcaaacata catgggggtt ggatgaccca tgaataacag gtaaaaagtc
    39181 tgcttctcat ctgctgattt tgcttgtgat tttggatgtg cgttgcgagg catcgaattc
    39241 atcctttgca ttcgagatcc atgtttgaag ctggtgttga ctttcaaatg ttacatatct
    39301 tatttgtgtg atttctctcg taggtttgca atactatacg gatttacaag ggaagtcaat
    39361 tattatatta ccctcttttt acaccctcca tcatgataaa agaatggcac agtggagagt
    39421 atcttactgt gccataaaag cctacaaacc gatattcttt tttaagtagg accgtaattt
    39481 tggcggcctg accgcactga catccacttt tttggcgcgg atcattctgg caaaacgggc
    39541 cagtcctttt gcgagagcac tggcaaaggc ctcatccttc ggcgcatcat cctccagcca
    39601 aaagccgagg atgtgaagcg tttcggtctg gcgatccagt ttggggtcaa ggcgtgccac
    39661 gaggtcgtca ccgtataaga tcggcagggt gtagtatccc cagcggcgct gatgcaacgg
    39721 tttgtacact tcccatagat attcaaaatc aaaaaccact ttggcccgtc cacgtgcgct
    39781 gaccatttcc agcggggcca ggaatgtaac ttcttcctgg gtggatgtgt cgagcagttt
    39841 ccattttttg ggaatacgcc ccgcttcaag atcagacagg tgggactgtt cactgtttaa
    39901 aacgagatat ccatcctttg agccttccac acgcagcctt gatgcaatgc cctgttcgat
    39961 gagccgttca atgcgttttt tgccttcttc ccgcgaaaca tcgcgatgga taaaaccagc
    40021 caaacctgtg cgccagacgt tttcgcgcgc cagaccaaga tgggcaatgg ttttacggct
    40081 gaagaaatcc tccgcttctg aatcgggtac ggtatgctca tattttttgg gaagaacccg
    40141 ttcggtcagg tcgtagattc ggtcaaagcc tttgcggttg gtgatcatca cttcaccgat
    40201 caaccacaaa taaaaaagcg cgatcgattc ttccttccga ccgcgatagc tccaactctt
    40261 aagcgcctcg ccgctaaaat cacggtttcc cagcggcccg cgtgtgcgca gttcattcaa
    40321 gacggcattc aataattttg caggcggttg ttgataggca tcatgataat aaataccctg
    40381 tttcttgcgg tgtttcatgt gcagccgcca gtaggggaat tcactcatgg gatacatgaa
    40441 tagccagccg ccatagtcaa aaaatccgcg tttttcatac gcggcttgat aaagattctc
    40501 tggtttgaaa tcgagtacgc ggccgtacag ggcaatttcc tggctgcgtg caacgatgtt
    40561 gagcggatcg agttgaatgc cttccatctg ctgaatggcg gaggtggtgc ctgccagccc
    40621 tttaaaccgt cggccgggcc aaagcccttg ttggccgagc aggtatcggc ggaatgtgat
    40681 gcgtgaaatt gtgtccataa aaaaataacc gaggagatga gtcctcggtc tccatttgtt
    40741 attggattta tttagtcatc acccagggag gcgatggctc gcagcaggcc aaagatcagc
    40801 acaccaagtt tgaggccttc tccggcggta agtgcggttt cgcgttcatt tttttctgcg
    40861 cggcgggtga ggagcatggc agcgatgaga cctgtgacag cgccgatcaa tgcgccaagt
    40921 aagatgtttt tgctgttgtt catgagttgc tgatccttcc aaaaaaggct tttagatttt
    40981 cgacaaggtc attgatggca aggatgggtt ttgcggccat gtctgcgccg cgggtgatgt
    41041 agccgcgggc tttgtggaca taatcctgtg cgatgccggt gtagtatgga agtccgctca
    41101 acgcacgggc catcaggtaa atgccgccaa tgagcagggc cagtacgatc aaccccgtga
    41161 tcagaagagg aatgatgatc cagatggtgg atatggcggc ccaacgcccc acatcgccgc
    41221 cggatttgaa ggtggcgaat gaaatcagaa cgatcatgcc gatcatcaac aaagccgaaa
    41281 gtacaacggg caggatgacc tgtgtgttgc gctgcttgcg atgcacgaga taggaataat
    41341 gttcagggtg tggaagttga gctttcattg cttatatttt agcatagtgg catttgtttt
    41401 actaagcaca ataaggttac ggcgtgctgt cttcgagcgg tgtgcgttcg ttgagcgcct
    41461 gcagcgggcg gttgttgcct tcaaataccg ttaataactt ttccagttgt gcggcagata
    41521 cttccacggg tgaggtcatt acgagccagc ttacaccttc agaacaaggt ggggttgtaa
    41581 gagacccgct gtaccggaag gtggtctgtt cggctggcag gaaatcggcg gcattgacct
    41641 tgctcccggc atctttggcg tcggtctttt cggcaggcag gttgttgacg aatggatcga
    41701 aggctgcatt ctctgtgcct tgttgaagca gaatgccaat gacagccagt ttcccctccg
    41761 catttttatg gacgatgtgg atctcggctg cgaatgcctg cccatcaatg tggtgttcgc
    41821 tgggagcatg ataatgaaat tgaaccacat cgtagcgggt cccatccaat tcaatataac
    41881 tgcccgtatc gtaattgacc tgggctgtgt gcccgttgtt taggatattc acttcactgg
    41941 gttggtagtg gaatgagatg ttgacgaggt ctttttcaat gggttttgaa atatcaatgg
    42001 gtgattgatt ctcacctgtg ccgcagacag cataggatgg gtcgatttct ccccagtgtt
    42061 gcgggccttc ctcgccttcg taggtccaat gcggggcttc ctgcggttgg tttgcacatg
    42121 cgcctaacag taaagatgcg atgatgagca gttttttcat acgggtctcc ttgataaagt
    42181 tatgcaatca ccaaggccta ttatccatct catttgcggt tattggcaat ataaaaaagc
    42241 aaacatgact tcccccgaaa ttaaggtttc gcggttaagt tcgtatctta agaacaaata
    42301 ggaagctgat ttacacacac ctgccctggc gggtggtgcc aagggaaaac gctgattttt
    42361 gcttttgatc agcgtaaatc agcgttttaa taaaactcag ggttaattat gtttgtctgg
    42421 ttattgtgct ggcttgcctt cgactggata tcctttggca taccattctt ttaacccgcc
    42481 agccatgctg gtggcgttgt atcctgctgc gagcaggatg tcacgtccct gctggctgcg
    42541 gtttcccgaa cggcatacca caagaatttc acggtctttt ggaatttctg ccagtcggct
    42601 ttgcagttgg tcaagcggaa ttagtgtggt gttgggtgca tggtattcat cccattcctc
    42661 ttgtgtgcga acatccacaa caaaggcgcc gttttgatac atttcatacg cctggtctgt
    42721 gttgacctcg cgtgacaatc cctgcccgct gccctgcccg gataaggcga tcaatacgat
    42781 gacaatgacc acaactgcga tcagagtcaa ctgcccggct ggtttgcgca gaaaagcgga
    42841 aagtccgctg cgggaggtgg tgtttctatt tttctttttt gacatatgcc ctcacaaatg
    42901 attttgattt cttttttagg acgtagattt tgcagatggt cttacacaaa caggggatga
    42961 gttttggtta aaaatgtttt cctccttctt tggtcttaat tgaccaggaa ggaggaattt
    43021 tttcaagtat ttgtttgtgc tgcgccttgt gtgacgcttt gtaagactgc tagtaagatt
    43081 tgaccgccgc atcaaggtcg tgatacacat cgaagaattg tttcaaccct acaacatcca
    43141 acacctgctc cactgagggc tgcgggccga acaacttcac atgctggcgg acagcaccat
    43201 cacgttcgaa attgatcgcg cgaaaagacg ggcgccccgt ttccttcgaa tacatggatt
    43261 gaccggcgaa gatcagcacc acggtgtgta aagacatcaa ccctgcactg ctgacatatg
    43321 agaccccgct caagtcgatc agaatatcgc gcacaccgct ggcgtataac tcctgcgcct
    43381 tgttaatgac ctcgatgtag ttggatgcat ccagattgcc cgaaaggtgc atgatagtta
    43441 caaatacgcg ccattcctgt tttgtgattc tgatttgcat gggttcctcc gggttgtaac
    43501 ggtgtttgca ttttacctga aattgaattc ttcaaagtgg atgttgtctg cggggacctt
    43561 catttcaatg aactttgctt cgaaggcctg caccatggga agcggaccgc acatgtaaac
    43621 atggtgcgtg ctgatgtcac cgcctgcatt tttggcgatc tcctccacgg tcaacgagcc
    43681 atctgtggag gaaaagcgga tgtgggtctt caaccgcgga tatttttgtg cggcggtttg
    43741 aatctcttcc acgaagatgg cttcttcacg gtgtcttacc gtgtagtaaa aatccacatc
    43801 gaaggcaggg tcagttttca ggtcccgaat gaacgagagg aagggggtga tgccgattcc
    43861 gcctgcgatc cagatctgtt tggggccgcc gctcttgtag tcgaacattc catatgaccc
    43921 ttccaccgta gcggacatgc ccgcctttaa gttcgcaaac aggtgacggg tgaaatcgcc
    43981 gcatgccttg atggtgaccc gcagcacatc ttcgtgcggc gcgctggaga tggtgaacgg
    44041 atgcgcttcg tccaaccctt cgcctgcgaa atgcacgaaa aggaattgcc ccgccttttg
    44101 atttttgatc gattcctttt ttgcgcgcaa cactacttcg gtggtggaat tgttcgggtg
    44161 gttgaccgcc tctactgtgt atggcaggta ttttttgaag agcccgccga agacctcggt
    44221 gtataaatag gaaagtgtgc caagaatgaa gaagttctgt gtccacgtaa tggcgatttt
    44281 tgcactgggg gcattgatgg tcagcgaatg gacgaagccg agaataaaaa agatgccgac
    44341 gaaacgatgt agttttttcc agccttcgta ggtttggccg gtcaatttgc tcaagaacgg
    44401 gatgcgtggt gccagggtcg gtagaacgat cgccacgatc cccagaaaag cgaccatggc
    44461 aaggtagttg cccacgcgca ggttgacgag tgtgatgggc acggtcagca gatgtacgaa
    44521 catcaacaga aatgcactgg tggatgtgtg acggtgggtc atgtacatct tgtccaggcc
    44581 gccaaaaaac ggctctgccc atttgggacg cgtggaaatg aacaacgaaa atgacatcaa
    44641 cacaatgacc agggagccga ggacttcccc tgcataggca cgtgcgtaat gttcagcacc
    44701 ctctgccaca ggcggaaaaa cgatccagat gataagtgtg agtgccacaa gaatgataat
    44761 ggtaaggttc ccgatatttt ttttcttcat cttgcctctg gttgatctgt tttgtcatga
    44821 atcaacagcg atgcagtgga aactgcatcg ctggggaaag cgcaaatact aaaaagaatc
    44881 gattgctttt tgcctgtcgg tgaaggtttc gaagaaggcg gaaaagccga ccatatcgag
    44941 cacgctgata acttcaggac gcggattaag aagtttgagg ttcttctgca tcccaccatc
    45001 gcggctgcgg tccatggatt tgagggtggc ccagccatgt tcaaggtctg gcaattcctc
    45061 gccgcgggtc atcagtgcaa tggtatgcaa tgaaaccaac cccgcgctgg aaatataagt
    45121 caggtcgccg aggtcaagca ggatgttcct tgtaccagcc tccacaactt cacgcgcctt
    45181 ggcgatgaga gtttgatagg tctgcccatc caaatgacct gccaacttga tgacagcaac
    45241 ggaaatgcgg ccttgctctt gtgagatggt aatttccatt tgaatactcc tttcaataaa
    45301 tgatgccgta ttataaacct gctttacgtt ccaatcgttg caggcggtgg tctatttctc
    45361 cgagccagtg tttgcggatg tatggaggca ggtcggcccg ttccacttct gcgcgtgccg
    45421 acctggccca tttgagcgag gtttctgcat cgcgcatttt gtgctcgtaa tattttgcca
    45481 gttcaatatg ggcatagaca tgaccgcgct gtgcggcctg ctcccacaag cggatggcct
    45541 ggtctgcgtc accacgtttt ttttgcagga tggataaccg tttcacggcc acgccgaaat
    45601 ctgattcgtt taacccaagc tcaagtccac gctcgaagag acgggcggct tcatcccagt
    45661 ggccgaggtc ttcgaataat ttgccgagtg caatgaaatc aagtccgtgt tccacacggc
    45721 cattgtaggg gtctgccagc agttcgctga catgtgccag cagggcggcc atggccacca
    45781 catccatggc gttgtgatag aacacgcccg ccattgggct ggcatcgcct gtacgcagat
    45841 agtcgaaata taaccaggga atttcataac ctggcacttc ttcagaagtg cgggtaaagc
    45901 cgagtacatg ttcttcaaga tacttcaacg ctcgcgaagg gagtctgtcg cgccacaacc
    45961 gccgtgccag cggaagcagg tcgaggtggg cataatcttt gaaagggacg gggatgcggt
    46021 gaagcgaata gcgggtgacg agcagcggcg catcgaaggc ttttccattg aaggttacca
    46081 gtccttcgca gggcgcgaga aaatcgatca atgcttcgag catggcgggt tcttcagacg
    46141 ggtcgcgcag gaagaattgc cgtagaacga acctgccatc tacaaaacga ccggctccca
    46201 caaggaaggc gtatgtccct gtgccgcctg ccatccccga tgtttcagtg tcgaggaatg
    46261 caaatttatg gatcggaagc tctgccatgc gcggatcgtt ggcccattgg gcaatgatcg
    46321 aaaatggaaa agaggaggcg taagccgttt tcccgtgcag ataatcttcg gcaaaggttt
    46381 gttcggcgat gaatgcctcg ccgcgcgggg tctggaaaaa tgtcccagcc acgaccgagt
    46441 caatggaacg gctggcaggt ttgggcgcgg gaagatccgc tgttcctgtt tttacaccga
    46501 gcgctttgag tttatccgca agcgatgaca tgggatgtat tgtatccatg actcagcact
    46561 ttgccaaatc gaaccagctt tgtatagggt gctataatga tttaaacaaa atcaatttac
    46621 ccccatgaaa ggatgtccct ttggtaaaag tcaactatca atacgaaaag cgcaaaaaag
    46681 aactggataa aaaacgcaag aaagagcaga aattaaagct caaacagcag aaaaaaaata
    46741 acccgtctgg tgaggttttg accgaggtgc aggatggtgc catggttgaa gccccagcgg
    46801 agaccatgcc cgtgaccccg cctgttgaag aagtggataa ataaaaatac gagaagcaat
    46861 aagaagagga gatggagttc catctcctct ttatttttta aaagttgaaa aacataataa
    46921 tcctgcaatt atgagtttct tgacaaatta gaacaagtgt tctatattag aggcagttcg
    46981 attgtttttt catctggttc caaaaaagga gatttcccat gaaccgctct ctcaatcttc
    47041 aattgaaact gcttcccttt ttgaaaagcg catctctcaa acgcggcgcg atctttggcg
    47101 gcattctcat cagcgccctg cttgcctttg agttgttcaa cttcggcacg acatcctttg
    47161 ccttgcagga tgtactcggt aatttgaaat tcgcgggtat tcaatggtct gccatccttg
    47221 cctttgcctt ttgcggaatt gactttgccg gtattgcccg catttttacg cctgagcagg
    47281 gtcgtgacga acccgccgag gtctggtacc tgtttggggc ctggctgctt gcggctggtt
    47341 tcaatgccat gcttacctgg tggggtgttt cggtggcgat cctcaaccac aatgccgaag
    47401 gcggctcttt ggtggggcag gcggtgatgg tgaaggctgt gccgatcttt gtggctgtga
    47461 tggtctggtt gatccgtgtg ttgatcattg gcacattctc cattgccggg gaacgcctct
    47521 tctcgctggc ggaagcaact tcacgacctg ccgctactgc gtatcaaaat aattcgccgc
    47581 gcccggttac acaatcacaa cctgtgtatc gcccggcagc catgagcgcg agttccagca
    47641 atcaaaatta tccgcgccct gcacccaaac cgcgcccggc accggtttca ccgaactatg
    47701 gtgccaaccg tcccgaacct acctatcacc ccattggtat gactgcaatg ggacatggtg
    47761 aaggcagcaa ttccacatct tctgtgcgga gataggacac gactgaagtc ctttaatcac
    47821 tgatactctc tcacgcccgt ttgggcgaac cactgaggcg cccaaacggg cgcctcatgc
    47881 gttaagggac tggtgtattt ttactcgaac tcgtccagtg tgctttcgcc gtgggtgagg
    47941 atgtaaagtc cgcgtggggt gccgttgttt ggatcgaaga caaaatcggt gacgatgaaa
    48001 tttttttcga atgcggtttc gaacaattcg cgggtgaaga agcgccagtc gcgggcgagg
    48061 gcgaagtctt gtgatttgag ggcggtaaag tcgcttggaa tttccgcgag cagcaggcgg
    48121 tcgtcaaagg gtggaacatg ctcgggtggt tgtggcaggc cattggtcga ggtgcgaagc
    48181 gggtagaact ggcgcaggcc tgagctggca acgtgagata acttgagggt ggggcgcggc
    48241 cgtttgctga gtcttctttc cacacgcgga gtgttgatcc accaatctac ttgaaatcgg
    48301 tcggagggca gaccagcgtt gagaccgtcg cgcatgtcgc catattcaga ccgccgatag
    48361 gtgacacaga ccgctcccag tttggagatg ttgagatagg cgttccgact gagaagaggg
    48421 tcatacgtcc atgtaacgtg gtcgaggtcc tgatggcgga ccatttgcca ttgggcccgt
    48481 ttgagcgaaa agccaacccc gccatcgcga tagtcaggat gaatgcccat catgtgcgag
    48541 caatgtttgg ggcgcgggcc gtccggtgtg gattcgagac cgggaaagcc aaagacaaac
    48601 ccgacaatct tttcttccac aaatgcgccc agcaccaacc cgccgttgtg aacggcggtg
    48661 atgagcatat gcaacggcac cacgtctgtt tcggagccgg gccatacatc gcgctgcagg
    48721 tcttcaacaa ggcgcatttc ttcgggagtt tcgatgaggc ggatggagaa attgttcatg
    48781 gttatcgagt gcggagcgct gcaattcttt ctgtaagggt gttccatgcg cgttgatacc
    48841 acggcttgcg gatcaggtaa tcgaggcggt aggtgaaacg cgcaggcatg aggccaaata
    48901 cccgcggcat cgaatccacg gcgcaggtgc ggttgacggc gaaataatcg agccagaaag
    48961 acgagatggg aaagttgggg atgacaccat cgagaaatac tgtgatggcg cgcagggtga
    49021 tcggcgaaag cggaaccagc acacgccgtt gacgggtcac atccatgatg gtttcaatga
    49081 tctgctgcag ggtgaaaaac tcactgccgc cgagctcgta cacgcggttg agcgtgtctt
    49141 cattttcaag cgaccacaaa atacaggtgg caaggtcttc cacccataca ggctgcacca
    49201 ccgtgcgccc gccggactga atgggaaaca accccaccga agcccgcacc agtcttgaaa
    49261 ggttattcgt aaaatggtct tcaggtccgt ataccagtga cgagcgcagg atcgtatatg
    49321 gaaccttgcc attgcggatg tgttcctcgg cgatgccttt tgccctgaag gcgggatagc
    49381 ccgaagagcg cgccgcgcct atgtgactga gatacacgat ccgatccaca cccgcatctg
    49441 ctgctgcttc gacgacgttc tcggtgcctt gaatatcggc tgttaaaaga ttgccgcggc
    49501 tgccctgatt ttccgcactg gcgaggtgaa taacggcttc cacgtcacgc atggcggcgc
    49561 gcaggccgcg cttatcggca agactcacca ccgccacttc cacaggcacg cctttgggga
    49621 ggttcggcgt ccgcggcgaa gggcgaatca aggcccgtag tggatatcca atggaagaaa
    49681 gctgtcgcac cagtgtacgt ccgatgaaac cggtggcgcc tgtgatgaga atcatggctg
    49741 ggattatagc agagggccgc aaacgctgac gcgtagcgct cgaaccccag ttactc
//
LOCUS       DAOQLC010000011        49225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_183_length_49225_cov_23.086109, whole genome shotgun sequence.
ACCESSION   DAOQLC010000011 DAOQLC010000000
VERSION     DAOQLC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 49225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49225
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183_length_49225_cov_23.086109"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PL000_03445"
     CDS             <1..354
                     /locus_tag="PL000_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC87979.1"
                     /translation="CLYLFVDYPSLQEELAVVHLKVPNLNPKLAQQAVEFVQRLRKAD
                     MRKSPSISETLDWANALVALNATNLDKDVLEDTLSVLLKHEADLQKAKRQLMNPPQNP
                     KQRPPADDFGRFSGN"
     gene            574..1776
                     /locus_tag="PL000_03450"
     CDS             574..1776
                     /locus_tag="PL000_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87980.1"
                     /translation="MPPKQFILISLFAILTLSLGVPSAEPVTVPQNIPTATVTVPPSP
                     TPTRTALPTPIPYYITATVWESQPAAAILTYHKFKTNRPSTYSTVSRLDFEEELESLY
                     RSGYVSVSLEKWLAGDIRVPVGKRPVIFSMDDVFFRNQIALTAEGTPAKDTGLGVAWE
                     FYKKHPEFGFHWALFSNLGDKPYGEGTSIEQKIQLANVIVWCIEHDAMIYNHTFRHVN
                     LKNTKGLGVTAELRWNDETLRELLQLVHRADLIPKLRNMVALPGGRAPRWEDSQTALY
                     GYTDPDGRKVQAIFNIDYITRPDFLKPPYARKFNPANLPRIVANLEAIEYLVENKEKY
                     PAAKSCRLGPLDQGQSADSVYLAGQVSAAVRRGQCPHGVYVTDKFIFSAETEQTSLIQ
                     EIQNDNQP"
     gene            1813..2706
                     /locus_tag="PL000_03455"
     CDS             1813..2706
                     /locus_tag="PL000_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC87981.1"
                     /translation="MTTQETFTRPTTIAEMQERMKNFATPEGWKKGLAYKPAPTDVFI
                     VTPPKCGTTWMQQIVHGLRSRGSMDFDEITRVIPWINLAHDSGIDIDAPQVAHPRAFK
                     THSTMDEVPQGGKYIVVLREPRDALLSHYYFFEGFFFEKGSIDIETFAKEYYLPGRAV
                     FKHIAALWERRKDKNVLPLCFENMKADLPRTVERVAEFIGITLDDELKEIVVRQADIK
                     FMQAHEDKFEDHIIRKSRSAAMRLPMDGQLSKVRDGQVGGAKLSVPENIKQELDAIWE
                     SELMPKIGLNSYEELKKELMH"
     gene            2738..4114
                     /locus_tag="PL000_03460"
     CDS             2738..4114
                     /locus_tag="PL000_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC87982.1"
                     /translation="MESRILQLIAALRASGVRVSLAESAEAFSAVDLMGIQEREAFRL
                     SLRATLIKDVKDIPTFDKLFPLFFGSGQPPMMGGNPTDDLSPEEIQMLAEALRQFKES
                     LRQNMERLMNGEPLTKSELEALGQLVGLNQAGDLNYQNWMAQRMMRAMAFPEIQKAMR
                     ELMEQLQEMGMNRERIEQIRNMVQQNMQGMQNQIEQFAGQRIAENLSEKPRGENIDNL
                     MNRPFQALSDADKKILQREVKRLAAALRTRIALRQKRMKSGQMDPKATIRANLKYHGV
                     PMEIKHRDKVRKPKIVVICDVSTSMRFCSELMLSFLFALQGQVRKTHAFAFIDHLESI
                     SEDFNGANADEAIQSVLWRMPSGHYNTDLGWALDNFNSEHMDTLNSGTTLIIVGDGRN
                     NYNDPRIDIFSTMTRRATRTIWLNPEPPAMWHGDSDMAKYAPLCSNVLKVGNLRELAQ
                     AVDELMTG"
     gene            4192..4569
                     /locus_tag="PL000_03465"
     CDS             4192..4569
                     /locus_tag="PL000_03465"
                     /inference="COORDINATES: protein motif:HMM:NF026926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm10 family immunity protein"
                     /protein_id="HNC87983.1"
                     /translation="MKFTAIYIEPYANTVTANVMFADPETDPDQPSVLSFSRPIEPGF
                     ENSSYYFEINDQSYGSYGGLETVQVSRNSLVVRVSPEKVQEFGEEDFREIQVDFQVDD
                     ETYGNVLAQLKRIFEGFDILQIQ"
     gene            4627..5007
                     /locus_tag="PL000_03470"
     CDS             4627..5007
                     /locus_tag="PL000_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87984.1"
                     /translation="MPWKISYLEDTKIIKTIYTEPITLDELKECAMANFSMAAEKQTG
                     LFLGDCSSFTQQGSTMDIYELGKFLESISLELRIHIKEALIEPSNIQRVSSDLRFFET
                     VTNNRMIKARIFQDTESALEWLLN"
     gene            5141..5584
                     /locus_tag="PL000_03475"
     CDS             5141..5584
                     /locus_tag="PL000_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87985.1"
                     /translation="MPISYDISNTFNLLVYICTDSWTGTAFFKTADKVPEDPRYCSNM
                     KIIIDIFSAEIETSLSDMHLAIEKNRQLKQKGQKLGQTAVFTKSTSLMFLADAFKLMS
                     PEAPSNFGVFNNKQDVVRWLGFTDAEKEVIRFWEGAKTSPPEKVP"
     gene            5629..6759
                     /locus_tag="PL000_03480"
     CDS             5629..6759
                     /locus_tag="PL000_03480"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC87986.1"
                     /translation="MSNQNIDTIIIGAGPAGLAVGACLKQANHSAIILEQDNAVGPVW
                     RRHYDRLHLHTDKKNSELPFAPFPKEYPRYLSRDQLVDYLEAYAKKFELNILFNQQVT
                     SARRINDRWEIQTNNAQYHASQLVIATGYAREPYIPDWSGKDGFKGKIIHSSEYKNGS
                     EFKGQRVLVIGFGNSGGEIAIDLYEHGARAGIAVRNAVNVIPKELAGIPILSIGIVQN
                     LMPVWLADAINAPILRAVIGDITQYGLRKLPYGPATQIRNDKHIPLIDIGTMKLIRAG
                     HIDVYSGVENFAGNRIKFTDGKEADFDSIILATGYQPRVNSFLQEQSVYDENGTPIST
                     GREAGVTGLYFCGYYVSPTGMLREIAMEARQISAAIARKRSL"
     gene            6907..7266
                     /locus_tag="PL000_03485"
     CDS             6907..7266
                     /locus_tag="PL000_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC87987.1"
                     /translation="MPKIMIVDDDPQVITLLKRYLSVEGYEVIATTHSAKAVPLANTE
                     HPDLFILDLMMPPPDGFTLCSMLRADPAFRLTPILIITAMADSNSKATSFGANDYLAK
                     PFNLDELSLRISTLLNG"
     gene            7402..7761
                     /locus_tag="PL000_03490"
     CDS             7402..7761
                     /locus_tag="PL000_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC87988.1"
                     /translation="MSKILIIDDDMETTKLLESLVKMNGHSPTSINNSADAIETANSI
                     IPDLILLDIMMPRINGIQLCKMFQSTQTLKHIPIIIVSALNDIGSKKDAFNAGAKDFI
                     TKPFLPKELAQKINALT"
     gene            7902..8273
                     /locus_tag="PL000_03495"
     CDS             7902..8273
                     /locus_tag="PL000_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC87989.1"
                     /translation="MTKILIVDDDKQATDLLQKVLTVEGYETTAVNDSSKAVQIADSV
                     NPHLIILDLMMPDPDGFKVCRMLRQTPRFIFTPIIIVTALDDSDSKVVAFGAGADDYL
                     TKPFHIDELSQRVKELLGRTE"
     gene            8419..9018
                     /locus_tag="PL000_03500"
     CDS             8419..9018
                     /locus_tag="PL000_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC87990.1"
                     /translation="MPKAFSEQEKDTIRLQMREKGKKLFEKHGLKKTSVDELTEAVGI
                     SKGAFYLFYESKEELFLEILEQFENEIQTQILEFTIAPKANAQKNVSEMLKGLLLTWD
                     AYPLLKNFSKTDFDYLVRKIPSERVMQHASNDEAFTTEFIKKIKREGINVKAPPRVVS
                     NLIKSLFFMGLHRDDMGETAYQETIGVLIDLVAGYIAGE"
     gene            9020..9928
                     /locus_tag="PL000_03505"
     CDS             9020..9928
                     /locus_tag="PL000_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC87991.1"
                     /translation="MEYVIETNNLSKTYGSINAVNSVNLRVKQGEIYGFLGLNGAGKT
                     TTIRMLLGMIRPSEGNVKVLGQVLGPGRSGPWAQVGHLVESPSAYPDLTVRENLDIAR
                     RLHGLKNSNAVDDVMESLSIASYSKRKAGALSLGNLQRLGLARAMIHHPSLLILDEPA
                     NGLDPAGVVEIRELIANLAKNGVTVFMSSHILTEVDKLATRIGIIHKGKMIEDLDANK
                     LEQFRARRLKIKVRDFARAHAVLTRENYSATITEDSIFVSDTRALETPEKIACLLVNA
                     DTPPTHLAVEQQNLEEHFMQLTGSTK"
     gene            9925..10713
                     /locus_tag="PL000_03510"
     CDS             9925..10713
                     /locus_tag="PL000_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC87992.1"
                     /translation="MNAYLAALQTESLKAFRSKVPLFTLLGFLIAPLIGGLFMVILKD
                     PEAAKSMGLIGTKAQLSAGVADWPTFFNLLAQATAIGGAIIFAIVTTWVFGREFSDHT
                     VKELLSLPTSREAIIAAKFTVIGFWTAALTLLSYGLGLIIGNAVAIPGWTNELFRSAT
                     IDILGAGILTILLLPFVAFIASIGRGFMAAFGWAIFTVAIAQVAVVMGWGDWLPWSVP
                     ALFSGAIGAHSETLGMHSYLIVAIASAVGFFITFYWWRNADQAK"
     gene            complement(10748..11911)
                     /locus_tag="PL000_03515"
     CDS             complement(10748..11911)
                     /locus_tag="PL000_03515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC87993.1"
                     /translation="MAKILYTAFDIVPSPKGASTHILHNLRGLVNGGHSAHLITPNDG
                     LLPTEDTIEGAQLTRISQDLSQNFLARAMHFGRSVAAHLALHQGYDVVHFRNIWDGLS
                     IAQNKKRLGYKTLFEVNGLPSIELKYHYPGLDPELLLKIKEQEIATLHLSDAIICPSN
                     VTRDYIASLGLDRKLVTVIPNGVSPSDFSPSSLPARDGRIPVLLYIGTLADWQGLDIV
                     VKALPKILEKQPVHLKIVGRGRSRQRKVLAKQIRKLGIEEHVTVQPAVPHHEIPALIA
                     SADICVAPLGLNDRNVTQGACPIKVLEYMAAERPLLASNMPIVRELVREDMDALLFSP
                     SDPEDLARQTITLLNDFELSKRLARSASERALTKFTWHESQKKLLKVYEKLLA"
     gene            11985..12929
                     /locus_tag="PL000_03520"
     CDS             11985..12929
                     /locus_tag="PL000_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC87994.1"
                     /translation="MPALCVLGVVGLVVVSLFYYLLNTARKKVKAPTNIAGKKEYAPV
                     YVSIADKPDSIMRGMERFKAEVQKTETAGDKWRWIPLMIFFVGLGMMAVDGVLLLFGY
                     FSLVFTSGGLLLWIAAFIMARSLRRSDTLDFPPRYKGTKDILYTLRDDLKPGSTFLGH
                     LDLTGAMLKNKVARETHDTQNRTTHHFRDEWLSLKAKLYDGNVLRISAIQKTKKRASY
                     WKRSRISGKSKLKPEKYKGTSHDLKVRIVVNPEAYQIVPNKEFRPGVNIGKYTISQLS
                     TDGGMINMVASSPFDEVDNEQILAILKSTYSLLQRKAA"
     gene            12930..14489
                     /locus_tag="PL000_03525"
     CDS             12930..14489
                     /locus_tag="PL000_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNC87995.1"
                     /translation="MSRKIIPFIIALVLSCLVMVVAGVFAFSGAVTAEKFGSDPVWSS
                     EYPTAESMKIIDLTGDGQDELFIQNAFGIRLYDPNGNVISDFTYDKPKTTLGDVNGDG
                     VEDIVVYHSGNFFSVDTIINGQQKTIASELPVFVPARVALIRFEGGSQIVLGDVSGQI
                     ASITTDGKIVWESDFASEEIRGMDDAKIGGQTYAAIASHDGTVGVFAANGSAVWQLEQ
                     EQLRRMRAFDLNADGNSEIITGGELGAFYIYSAVDGSTIFSKSLGQAVSEVREVELNG
                     DPSSREIIVGGKDGGIWAFSYNGTTATQMWTASLSDKVTEISGLDINEDGKEEAVIGD
                     DSGKVAVFTENGTRNNLPSHNTGITRIDIGKLGNERYVVIADGSHVQTNKVNFNSIPG
                     FQFTPLIVGLVVSAIMLVIAGVLASIPPKPELKVSFQDKSKESLEAQRRMLKESIADV
                     ERLRKAGEVTGDAYLARLKRLRSDLAENETAYKTAGYTIKVETFQCPHCGGTLELGMD
                     KCEYCGHVILS"
     gene            complement(14582..15556)
                     /locus_tag="PL000_03530"
     CDS             complement(14582..15556)
                     /locus_tag="PL000_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC87996.1"
                     /translation="MEYRKFGKTGLKVSPLCMGTMQFGWSLGEAESHKILSATFEAGI
                     NFFDTADIYSKWVDGNPGGISEAYIGTWMKQNKIPRDQIVIATKVRGEMGNGPNNEGL
                     SRVHIMNAVEGSLRRLQTDYIDVYQSHWTDESTPIEETMRAFDDLVRQGKVRYVGASN
                     YAAWELMQALWVSDKLNLARYDSLQPHYNLIYRDEFERELRDVCKTYEIAVIPYSPLA
                     GGFLTGKYRRNEPLPESKRAEGRKRAMTEKNFSLLYEMESIGLRHKASIAQVSLAWML
                     SDAVITSPIIGATTIEQLQENLGALNVHLMDDEVATLNKLTAWKTDED"
     gene            complement(15731..16138)
                     /locus_tag="PL000_03535"
     CDS             complement(15731..16138)
                     /locus_tag="PL000_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC87997.1"
                     /translation="MKLKNLSLLLVVAVLFASVLACGSTAPAGVSNVYMANDEEGNNK
                     TTTFAPTDVIYVFFDVNGIDEGAQFQVKWYALTVDGQDPNEAFTTTDYTYTDEATIYA
                     NIESTDGGFPAAQYKVEIYLNGAKVGEQQFSVQ"
     assembly_gap    16193..16292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16366..17091)
                     /locus_tag="PL000_03540"
     CDS             complement(16366..17091)
                     /locus_tag="PL000_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNC87998.1"
                     /translation="MTELLYQTDAYLKEFSATILSVDSENRSVILDRSAFYPGGGGQP
                     CDFGTLEVAGVVYPVEKVKKQGDDVLHFLGGTEPLPTSNSASRCAGTLDWVRRHQLMR
                     THTALHILCGTVFRDYGAKVTGGDMDPLKGRMDFEFETMRGELVRDIEAAVNLEVQNR
                     RDIRVKILPREEAFQIPDLIRTKINLLPEGILQVRTVEIVGLDLQADGGTHVANTAEV
                     GRVKVVDYKSKGAINKRLYIEIE"
     gene            17277..18206
                     /locus_tag="PL000_03545"
     CDS             17277..18206
                     /locus_tag="PL000_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC87999.1"
                     /translation="MIKVSGLTKDYGARRAINNLKFDAQQGEIVGFLGPNGAGKTTTM
                     RILTGYMPPTDGEAIVAGYNVVEESLEVRKRVGYLPETVPLYTDMTAFDYLKFMGELR
                     KIPNIDDRVEEVLDMVGLIDRADSYIGNLSKGMRQRVGLAQALIHRPEVLILDEPTIG
                     LDPGQVVEVRELIREIGKERTVLLSTHLLHEAQNICDRVLIINKGKIVTEDTTQNLQS
                     RLIGGERVVVRVRGEADANLVNLIKAVKGVEHAVLTKDNSVEFDFVSGKDARPEVAKQ
                     VINSGYDLLELHPLGMSLEEIFLELTGSETKNN"
     gene            18218..18958
                     /locus_tag="PL000_03550"
     CDS             18218..18958
                     /locus_tag="PL000_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88000.1"
                     /translation="MRNIWTIAKREYDNYFNSPLAYVVAFAIVLPIGIYFALIMFVSA
                     QQTMMGGPAPDSTPINWLFVFLLIFLSPALTMRLLSDEARMGTLELLLTAPVRDFELV
                     AGKFLGAFMFVLTIALFTLIFPIIIDNYVTPGVDWMIILSSYIGLILISAALLSLGTG
                     ISAIFTNQFAAFFVTFGLFFFLWFMVNLPASYLQQGGDVYNYLSLSQHFSSMNRGSIA
                     LGDIVYYISLIALGLFAGTTAIEIRRWR"
     gene            18958..20571
                     /locus_tag="PL000_03555"
     CDS             18958..20571
                     /locus_tag="PL000_03555"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNC88001.1"
                     /translation="MIDNKNNSPAKYAFIGLIVAGLACVSSGLTGIAKGMVSQQIFTL
                     EETLTKNIDLAFNISLALIVIGLAAYAIMVPDQIRRFLTGRQARYGSNSLILIVAFLG
                     ILITANVLVFRNPDFLKWDMTEDKSNTLAPETLQALETLPGDVTATAYYSVNLDNASA
                     EELLTKFKNNSNGKFTYKFIDPDQDPVSAREAGITGDGKILLQMGETKEIVSFASESE
                     LTQALIRLINPGERTVYFLQGHGEPSLEAGGGQLSFSIAKSTLESKNYTVSPLNLIAT
                     NKIPEDALAIIIAGPLKPISSGEMVLLKDYMEAGGSLVVMEDPTIVTEFGDTFDPLAK
                     YLDSDWGIKLNEDMVIDLVNTQNPLQAVSSQIVDHPITQNLTENYIVILPQARSISVS
                     QEPIENVSTTPIILTTDQSWGETEMSAETQPEFSPEKDTPGPLNLAAVAENSATGGRV
                     VVFGNSIFATDEIFDAYGNGNMFINSVDWAAEQEDLINITPREQTQRMFTPPTNGVFI
                     IMIIISIFVLPGLIVFSGVSSWIARRKKG"
     gene            20575..21315
                     /locus_tag="PL000_03560"
     CDS             20575..21315
                     /locus_tag="PL000_03560"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNC88002.1"
                     /translation="MPRKETKPTAKASPRRKKTNVVTESTFTRESTGTQIQKPVIGIG
                     TWIALVLFAGLVTFAIFLNKKEETAVEATPTSGTVPLFPATEGTPSSIEVKPAEGEAV
                     RVLRNEENVWVLELPFETEADPGLSEAAATQVSALQEISTVQGDLADFGLENPAFVIT
                     VGFASGKTHTLEIGDMTPSNRGYYVRVDEDRLVVVAVSGLDALLQLQFSPPYLNTPTP
                     TATTPPPTETPTPAPPTETPAASVTPTP"
     gene            21486..21731
                     /locus_tag="PL000_03565"
                     /pseudo
     CDS             21486..21731
                     /locus_tag="PL000_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            21798..22700
                     /locus_tag="PL000_03570"
                     /pseudo
     CDS             21798..22700
                     /locus_tag="PL000_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            22773..23972
                     /locus_tag="PL000_03575"
     CDS             22773..23972
                     /locus_tag="PL000_03575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNC88003.1"
                     /translation="MKINIDLSRTAITAILISLALVYPLLWLKMLADPTQHTGADFIA
                     FYAAGRIADQEGPSHAYDLILQKKYQEGVVQREIELQETFPYIHPPFVIPLAQIVSNT
                     DYLKSFQRWALLMLLLFTAGIPFLIAPARKFFSQPQTLIFAAGIFLFFPIFQSLLLGQ
                     DNAIIFLGLSLLMWGTYAKKDWAAGLGLSLATVRPHFAFFLLLPFLFQRRAIFGWFLL
                     FVFALTLFSFGVVGTDGLKGFLHILTVSGSGEGYRTSEENMVNLIGLLRRLFPFLSAA
                     LIRSAGWAFYGLAFFSTGVYFWRNHTSPSGEEISLAVVAALFFSPHTHAQDLVLLIAP
                     IMAVILILLERQRIQPTQAVLIPLAISLLLLFSYYSPILTHSIPYLLMAGLFFAVKQY
                     KALPAKP"
     gene            23969..25423
                     /locus_tag="PL000_03580"
     CDS             23969..25423
                     /locus_tag="PL000_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88004.1"
                     /translation="MTRTERFFITIVFLLVATIAINPPVDNDMWWHLRAGDEMWHQGR
                     ILLTDQFSYTKQGADWVNAFWVSDLALYGLWQVGGFFAITLTTALLAIFTLWIIFKRM
                     TNAIIPRGLLVILAMMGIIANWTPRPQLLSFLLLAWLDSFLYEHIHTKRQPLWKLVLL
                     FALWGNLHGGFIWGALLLFATLAGESFDLLFGNSPKLVFKEIGHLALWSLAAFLAVAF
                     NPNGFELWKLPFQQVDVSLAIQEWLSPDFHQIYAHPMLWLMFLLVFGLGFSGKRLSFT
                     DLFKGLGFAYLFFFAQRNLVAYTIIILPIVEKFLAPAWANLTEAPQVKAFLERFSRQA
                     SKGQFSPGVAGVINNAVIGMFTILLVGYAWSVSMPASIAKEVPAQAVAWVKEHQPSGP
                     IFNSYNWGGYLTWSLREYPVFIDGRADLYGNDLIQEWVEISNGTETGLERLESRGINL
                     IFLEPYHTLLEKLDPQTWVQVYSDSQIVIYQRRP"
     gene            25420..26949
                     /locus_tag="PL000_03585"
     CDS             25420..26949
                     /locus_tag="PL000_03585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC88005.1"
                     /translation="MKQAQSHEKDVTGFFLLLAVVFGLAVRLYPILKTDFPLVDGGMF
                     YTMVNDLRAEGFSLPLVTSYNQADIPFAYPPLGFYLAGLINLLTGISVLNILRWLPFL
                     ISMLNIPLFYFMAERLIESKPKAALAALIFALTPSAYWWNIVGGGLTRSLGVFFFTAV
                     AFCVHQMFQRRSWSWVVAAGFSAAGCVLSHPAWAVQAAVASLVLWYFFGRDRQGVFYA
                     VIVAISVLLLTSPWWFNVIRHHGLETLLHAGQGSYSRLKIWTVFFTLSFTDEYTPVIA
                     VFALCGIFMHAARKDYLPIAWAAMGLLAEPRGGLPASTLPFSIMAMTVLSDGIATRLT
                     PSTEWMDSLKQHSGRSFFGFFILLFTYNAFQVSNTLSYQVLTKEERTAIEWVRSNTDP
                     ADRFLVLDDEGNPLHSPLTEWFPALSERRSVATIQGTEWLSGKSSYANQYDVVKNIQQ
                     CLYADMNCIHNLENLLTDEYSYILISTAGGQSPLGSDLNTQANAALVYSSTSVKVYRV
                     K"
     gene            26946..28445
                     /locus_tag="PL000_03590"
     CDS             26946..28445
                     /locus_tag="PL000_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC88006.1"
                     /translation="MKSQKAKLILVLVIFLLTRLAGLVTRPIWYDEAFSILFSEKGLN
                     AMIYGTLSQSGSGAADIHPLGYYVFLWAWMRVFGTSILAARLLSIFISLAALVLVYLI
                     ARELFDETTAWISALLFSILPFQVHFAQEIRMYMLLSFWLLAATYAFLRGRSGEWKWW
                     LLFGVCSVLAQYTHHLASVYLIPLALTPLWQKDWKTLKSVMAGALLAVLLYIPWLIHL
                     PAQLSKVQSSYWVERPGLEKIFTLILFYLPHLPLPNGLLLPGLLIAMLVIVLAVFQTI
                     LAKRNLIANTGKATWLATLAFLPPLLLWVISQFVPVYIERALLPSHAIFCIWLAWAFT
                     QTRLPNPVRIFTFTLIGIAAVIGLSQHFFYQGFPYGPFREINQSIQSRLENGDAIIHS
                     SKLSYLPSFYFNRKLVQTFIIDPPQSNVDTLAPATREVLQIRSAEDIETASANATRIW
                     FIIYQQSVDEYTAQGYKTHPHLEGLEENYELEFIENWDDVRIYLYVKKP"
     gene            complement(28481..29797)
                     /locus_tag="PL000_03595"
     CDS             complement(28481..29797)
                     /locus_tag="PL000_03595"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HNC88007.1"
                     /translation="MKYSLRSSFARLTLSLFIMVFLLTIVGRIVTVTGAWAYCLGWPL
                     CVPSEPIGYLKLAHLILVGLAMVLMFWLLRKAWREQRHQQVILPLTTVTGVLFFGQAF
                     IGAIEVTRSYPTHLVVLHALTAVALWISLGSLVYLSGALAVDSNEVVKFDYRQRAKDF
                     FILSKPIIVLLLLVTTYSGLVAGSKAWPSASLTFWTLLGGTLAAAGSSALNQYIDREL
                     DKNMQRTSKRPLADGRLTAAEGLSYGLALCLTSYYVMAGFVNLLAALLSLAGIFYYVF
                     FYSVWLKKATVQNIVIGGGAGAIPPMVGWAAATGQLGLAAWILFAIVFMWTPPHFWAL
                     AIVRMKDYEKAGVPMMPVVHGEEKTRRHILVYTIELIAVTLLLPIFNLAGMVYAVSAI
                     VLGGFLLYAAWRVFKVGGNKVAWTMYRWSSMYLAFIFLALMIDAVV"
     gene            complement(29803..30537)
                     /locus_tag="PL000_03600"
     CDS             complement(29803..30537)
                     /locus_tag="PL000_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HNC88008.1"
                     /translation="MMVLRKAISRKWFFASLLVLLGTAFCIRLGFWQLDRLEQRRAFN
                     AQVESMRAAEMLDLNQAIPQDIAAMEWRAVTVTGEYDFENQVAIRNQYNGDQYGYHLI
                     TPLLSNGTAVLVDRGWIPADGNATPADWRQYDEQGTLTVTGQIRLGHGKPAIGGIADA
                     LPAAGTRLEVWNNLDVENMSAQFPYPILGVYIQPNVEPADEVPPIPYQPIIELTEGSH
                     FGYALQWFTFATILFVGYPFYLRKQE"
     gene            complement(30506..31030)
                     /locus_tag="PL000_03605"
     CDS             complement(30506..31030)
                     /locus_tag="PL000_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88009.1"
                     /translation="MNFSIKRIVISFLLGLVLAAITTEAAYFFLKKENRAPGLIELVI
                     PAGTADSIRAGNAPVEIPRDMRFVVGDTLRVINQDDENHQLGPLWIPAESSASLKLET
                     AENLIFECTFQADNNFGVTVMEPVTWRTRVSGIFFAGFPLGMMFAVYGGLIGEKKKKD
                     NEKKDDGSSQGNLA"
     gene            complement(31027..31638)
                     /locus_tag="PL000_03610"
     CDS             complement(31027..31638)
                     /locus_tag="PL000_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNC88010.1"
                     /translation="MDKRITFVGLASLGIVALAFVLNIFFLKPASFRGALYGEPYPRA
                     SEIELPMANGQTFRLTEQHGKVILLFFGYTSCPDVCPTTLAEMKMVMDQLGDDGSRVQ
                     VVFVSVDPERDTPEKIQTYVEHFNPAFIGLSGSSEQLEPVWQAYSIFREAVQSDSAFG
                     VIINHTARLFLVDSQGNLRLSYAYQTPVDDIVHDIKLLLEREP"
     gene            complement(31741..32526)
                     /locus_tag="PL000_03615"
     CDS             complement(31741..32526)
                     /locus_tag="PL000_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HNC88011.1"
                     /translation="MSHTQTKSAAYGQGVVIFIYLAILTALEYFVAITFNAVSILVVV
                     AVIKAALVMYYYMHIYKLNEDSEGDEHSYDYKTGTNRIGLWLFLLSDGFVFAGLLVTR
                     INLLGLTRPHLNQTLGLAVTAVLLVSSFFMNRGETAMAHGDKKGFMNNTLITFVLGLG
                     FLLGVVMVEWRLAAEEGLTASFGNPAVGPMGGVFYIMTGMHAFHVLTGLIFLLIVWNN
                     ARKDVYSAEKHWGVEAAAVYWHFVDLVWIFFYPALYLIGKLVN"
     gene            complement(32541..34436)
                     /locus_tag="PL000_03620"
     CDS             complement(32541..34436)
                     /locus_tag="PL000_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC88012.1"
                     /translation="MKRFISSALAHGLLGQLIGTVVGGGLVTGIRALMGLSVTDRFFF
                     TEPAWVLGGFIGAIAFLFSSGVTSDWVKWARGIDTPEHHEDHHSGWEKFVNVSLDHKV
                     IGIQYTIVALALISIGGLFALIFRTELAASELQFLTTDMQLFGQNGPQLYNTLMSLHG
                     MIMIVSILLGIAGIINYVVPLLLGAQDMAFPRLNAFSFWIAVPAAVLLLMSLFLGGFD
                     TGWTGYPPLSARAPVGMQMFFLGVFVAGWSSILGALNVIVTVLRMRAKGMSAMRMPIF
                     VWAAIATSLIALTATQFIGLSFQLVMFQRLFGMGFFDPAKGGNPVLFQHLFWFYSHPA
                     VYVFILPGLGVISELLPVFVRKPLFGYRWIAMSSLGIALVGFVVWAHHMFTSGMNEYL
                     RVPFMYSTLLVAIPTGVKFFSWVATLWGGKISTPTPMLFVLGGIVVFLMGGLSGPPNA
                     IVSTDLHLHDTYFIVGHFHDTIFGGFVFPFFAALYYWFPKATGRRMNETLGKVHFWLM
                     TPSFFVLTFGMMYVGLLGMRRRIVDYDPALGLDATHLVLTIAGYLIALSVVIFIYNFF
                     SSIKNGEKAEGNVWNSRSPEWQVPSPMPVHNYDQPFEVISEPYDYGLEGGHAYIKFSA
                     PESGAHK"
     gene            complement(34457..35473)
                     /gene="coxB"
                     /locus_tag="PL000_03625"
     CDS             complement(34457..35473)
                     /gene="coxB"
                     /locus_tag="PL000_03625"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HNC88013.1"
                     /translation="MRHFVIVGILVIAMAVLTYVGLDAAGFATQMNPVAASAQAQTID
                     NMWHWEIVAMSFLFALIVAPMAYSLVVFRKRKGDDTDAQHVEGNTALEIGWTVGPLIL
                     VVIFAYMGAYSLGEVRRVDPEAVVIKVKAQQFAWTFEYPEYGVVSNELHLPANKQVLL
                     KMESADVIHSFWVPEFRVKQDVVPGRVTEYRITPTLIGVYKVRCAELCGTSHAYMEGM
                     IVVSEEANYEAWIAEQIAIAAAAQTPEGKGQLLTVSKGCVGCHSIDGTAMTGPTWFGL
                     FGADVKLADGSTVTADEAFISESILDPNATIVEGFPSPSVMPPFALTEEEIANIIAYL
                     KTLK"
     gene            complement(35649..36113)
                     /gene="bcp"
                     /locus_tag="PL000_03630"
     CDS             complement(35649..36113)
                     /gene="bcp"
                     /locus_tag="PL000_03630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HNC88014.1"
                     /translation="MPIPSGIPAPEFEMLDDTNALRKLSEFRGKNVVLYFYPKDDTPG
                     CTKEACNFRDDYSAYEKAGVVILGVSPDDVASHVKFKKKFQLQFPLLADDGHKVCDAY
                     GVWGPKKFMGKAYEGVLRTTFLIDGEGMIKKVYENVRPAEHSAELLKEFGVS"
     gene            complement(36217..36615)
                     /locus_tag="PL000_03635"
     CDS             complement(36217..36615)
                     /locus_tag="PL000_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC88015.1"
                     /translation="MKKVLFAVLVLAALALAACGGGSNAVATLAPVPAEYAGQANPLA
                     ADAAAAGAEVFKTNCESCHGPQGHGDGPAGVALDPAPKNLPELAAQAGDDYLYWRINT
                     GKEGTSMVAWKGVLTDEQIWQAIAFIRTLK"
     gene            36786..37283
                     /locus_tag="PL000_03640"
     CDS             36786..37283
                     /locus_tag="PL000_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88016.1"
                     /translation="MNALTLVLRLIHIVAGVFWVGAAWMLTLYIVPSATAVGDAGQQF
                     LRQMMGKTNFQKIMTTSSLSTVIAGTILYVRNPPTWFSSNAGIGFGIGAFFALIGLVF
                     GFLIGKTNMAMMQIGSQIKGQPTPDQIAQIQDLQKRQRTFSTINIYALLAAVVFMAIS
                     RYLFI"
     gene            complement(37392..37616)
                     /locus_tag="PL000_03645"
     CDS             complement(37392..37616)
                     /locus_tag="PL000_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88017.1"
                     /translation="MAENKFLEADMLALQLLSEGGHGPDTSLSWLLYIGIAFFVMIIV
                     FGWPEGSKKQGQAEGPREAPKGKKKADDLD"
     gene            37722..38600
                     /locus_tag="PL000_03650"
     CDS             37722..38600
                     /locus_tag="PL000_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNC88018.1"
                     /translation="MKLKTWQKILGILLIATILLRVTSRPAVDHPYYAADLNYPLVIA
                     HQGGDGLWPGDTMFAFQNAVDLGADVLEMDVHITKDGTLVLMHDETVDRTTNGTGEIE
                     SMTLEELKQFDAGYDWTPDEGATFPFRGQGVTIPTMEEVFAAFPDKHMTIEIKKTNAS
                     MAGPFCELIRKYNMEDKVLIASFHDDRLKEFRKECPEVATSSAKNETTVFVLLTKPFL
                     GSFYSPKFFSLQVPEESGGITVMTPAFVKAAHERNLAVEPWTINDEETMKKLIAWGVD
                     GIITDRPDIMIKVLGR"
     gene            38607..39422
                     /locus_tag="PL000_03655"
     CDS             38607..39422
                     /locus_tag="PL000_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNC88019.1"
                     /translation="MTSYEDSIENHRIQRDEKTAANQLHWLNLAGLFWLEKGENSFGN
                     GTDNKICLPAFPSAHCGSFHLENGIVTLHPAKGADITVNGKTPNASPLRTDREKDPDL
                     ISIGSLTLKIIIRGGEPLVRIWDRESPALKQFDGFHYYPPKPEYRVIAKYVRYDPPKP
                     IKITEVIGTEVESAFLGQARFTLNGVECVLEAEKSDDELLFNFTDGTNKDTTYGGGRK
                     FYLPIPKGDEIVLDFNLTENWPCAYTPYATCPIPPRENRLPIKVEAGELKYHD"
     gene            complement(39486..40046)
                     /locus_tag="PL000_03660"
     CDS             complement(39486..40046)
                     /locus_tag="PL000_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNC88020.1"
                     /translation="MELFEAIHGRLTIGKVKPEPLPRDVIEKLLSAGAQAPNHHKVRP
                     WRFVVLTGDGRKKLGDVMAASFLDRNPATPPEGLNKPRSLSLRAPLLIAVGADKPAAD
                     SKTIEVENLSAASAACQNILLAAHALGLGAIWRTGEWARDARVKEFLGFSTDQHIVGF
                     IYVGYPEVTPEPYTRIGFEDRTVWME"
     gene            40093..41130
                     /locus_tag="PL000_03665"
     CDS             40093..41130
                     /locus_tag="PL000_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNC88021.1"
                     /translation="MKIFYSEEHRSHYPPFEVFDGGKRIPYYENPDRMDRILAALGKT
                     DWAELTEPQDFGLDPILAVHDGNYIQFLASAWTEWLASDPQVAASPEQNAFLPATFAL
                     RRKPRVPNSLLGKAGYYIMDLSACIVEATYKAALASANCALSAASSSLILQNASFALC
                     RPPGHHAGKDYAAGYCFINNAAVAANWLSQKGKVALLDIDYHAGNGTQDIFYERADVL
                     TISLHGDPDFEYPSYIGFADETGAGTGLGFHKNFPLPKGTDDVRYLAALDEALTMIRN
                     FAPQHLVLSTGMDTFDGDPLGTFHVTRDGFSEIGKRIAALNLPTAIIMEGGYANEALG
                     TNTVTLLEKFK"
     gene            41127..41900
                     /locus_tag="PL000_03670"
     CDS             41127..41900
                     /locus_tag="PL000_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88022.1"
                     /translation="MRKGLINYLICAVIVFGCSLPSQAPTATPLPPTATETPAFTNTP
                     EFTATPLATLTSTLTLTPTITFTPTITATPTFSFPSVTVNKQAHCRYGPSVAFLHAAD
                     LYPGDTGYVDGRYEYSSWLHVKFDKLKYYCWVAPSVVDVVGDISTLYYNEPDLQSVGY
                     SNYPPPQNIVAIRNGDEVTISWNQVEMTTDHDRGYLIIAFVCQNGAYLWWTFSYPDQF
                     TTSYTVQDEAGCAQPSEGVLYTVEKHGFSKPAQIPWPAP"
     gene            complement(41897..42256)
                     /locus_tag="PL000_03675"
     CDS             complement(41897..42256)
                     /locus_tag="PL000_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88023.1"
                     /translation="MNQQEKTTMAPDEKTGGWKTGCLTGFLSGGVGVVGGVAVALISG
                     YFGFDCFADSQFFIEVFIFPVVGLLAGAITGAAAWLGITLSKGKTSPLVVGGIIYFLF
                     FCILAVIVAAVAKAFSC"
     gene            complement(42362..42730)
                     /locus_tag="PL000_03680"
     CDS             complement(42362..42730)
                     /locus_tag="PL000_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88024.1"
                     /translation="MEDGLIIDDNRQTADALQQMLGVLDLPARVAYGSSPAISMLSSF
                     VPGFICLDINMPGVDGTEVLAFLRREPRLMKIPVIVITSDDQPETRQRVMRGGAQAVV
                     IKPATLDALENAFKRSGVLS"
     gene            complement(42735..44717)
                     /locus_tag="PL000_03685"
     CDS             complement(42735..44717)
                     /locus_tag="PL000_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HNC88025.1"
                     /translation="MSVYLHDIPLAQARARIREALQEANLWRVLGVEEIPLNENALGR
                     VTAEPIWAEISSPHYHASAMDGFAVRAISTNGAMPSSPVTLHVGPEAQYVDTGDPLPE
                     WTNAVIPIENVESLDENDQITKEIRKPASIRIRSAVAPWSHVRPLGEDIVATQLVLPA
                     GHALRPADLGAIAASGHQTIRVALKPKVAILPTGTELVPIGSKLKSGDILEYNSLVLA
                     AQIKQMGGEPTRYPITRDDFGLICERVFEASQSHDLVLLNAGSSAGAEDFSSKVVEKL
                     GTLLVHGVAVRPGHPVILGMVNRQSENLKPETLNLIPIIGVPGYPVSAALTVDIFVEA
                     LIAKWLGRPPMELPTETATLTRKLVSPAGDDDFVRVAVGQVGDKLLAAPLSRGAGVIT
                     SLVQADGLALIPSGVQGMEAGEPVKVHLYRSKAQIEKTIFCIGSHDMTLDLLAQFLAE
                     HDRRLASANVGSQGGLVALRRGEAHMAGSHLLDPSDGSYNIAYIRQYMPNIPVKVVSL
                     VGRDQGLIVKKGNPKGIKSLGDLTSHEVQFVNRQRGAGTRVLLDYHLNLMGMSSESIS
                     GYTQEEYTHLGVAAAVASGRADCGLGIAAAAQALELDFVPLFQERYDLVMPKVFAESE
                     LLAPLFDLLTDPVFRNSVSKLTGYDVNVMGRIIMED"
     gene            complement(44795..46132)
                     /locus_tag="PL000_03690"
     CDS             complement(44795..46132)
                     /locus_tag="PL000_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC88026.1"
                     /translation="MTNQETQNWIEKDKRQLHPTYHPKSHANPLVIERGDGVWLYTTD
                     GQKILDGMAGLWNVSAGYGREELAKAAYEQMKVMSFTSNFSGMTNLPSITLADKLAGF
                     AYEGLNTTFFTSGGSEANDSAFKTARYYWKRKGKPGKYKVIARKGSYHGITLAATFAT
                     GLEKYHTMFGPAMDGFVHIPAPNPYRYEGQLKAGETVGQAAARELEEAILREGEDTVA
                     AFIAEPVMGVGGVIVPPAEYFPLVRQICDKYGVLFIADEVITGFGRTGEWFALKHWNV
                     KPDILSFAKAVTSGYAQLGGIQISDEIRETMENTADTEAYMHGYTYSGHAMACAVGLK
                     NIEIMEREEFPKRARELGKRLYDGLQSLKEFSFVGDVRGLGLVCGVEIVSDKEARTGD
                     AAKTAQIFKAAQDRGLRTRPLGSTLAFAPPLSINEDEVDEIIKRLGAAMDASA"
     gene            complement(46240..47679)
                     /gene="guaB"
                     /locus_tag="PL000_03695"
     CDS             complement(46240..47679)
                     /gene="guaB"
                     /locus_tag="PL000_03695"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNC88027.1"
                     /translation="MKILQDTALTYDDVLLVPQYSDVDSRRKLSTQTKLTKEITLQIP
                     IVSANMDVVTESEMAIAMAREGGIGIIHRFLSIDEQARQIQRVKKAESFIVDNPISMS
                     TAHTVGDIKKLVDETGAGGILILDAEEKLVGIVTTRDLLFENDDKKSVTDLMQKKVIT
                     ASPDISLKEAEGLLHEHRIEKLPLVNADGRVAGLVTLKDIMKITQYPKATKDSRGRLI
                     VGAAVGVRDKEMRRVESALKAGADCIVVDIAHGDSNLEIDMIKNIRKHFPAAQIIGGN
                     VATADGTKRLIDAGVDAVKVGVGPGSICITRMVAGSGVPQLTAVIECAEAARSAGIPI
                     IADGGIRQPGDVAKAIAAGAQTVMIGSMLAGTDESPGMVMTRRGHRYKASRGMASREA
                     NIDRNRKEGNDLTQEEIEEYVAEGVEAAVPYRGRAREVLTQLVGGLQSGMSYSGAHNF
                     EEFQKKAIFVRMTGAGLKESGPHDVEVLG"
     gene            47872..49149
                     /locus_tag="PL000_03700"
     CDS             47872..49149
                     /locus_tag="PL000_03700"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNC88028.1"
                     /translation="MTVTAVIGSQWGDEGKGKVVDYLAEQVDYVARFNGGNNAGHTVI
                     NEFGTFKIHLVPSGIFAKNTVGLIGGGVVIDPAVLIEEIDMLNKAGVGVDGRFWISPR
                     SHVIMPYHKILDGLYEEAKGAGATGTTRRGIGPVFADKVSYNGIRWTDFASDAFENRL
                     KVQLELKNKIIVALGGGALNYESVRDEYRGYYSKLKPYIKELFSLVQDGLKANKHFLL
                     EQAMGTFLDTDWGTYPFVTASTTIPSAASAGLGIPPRYITNVVGVTKAYTTRVGAGPL
                     PTEILDTKSAEYEQFGEVAATTGRIRRVGWLDLEIVKTAAQLSGVTELCLTKLDTLSG
                     LENLQVCIGYKLNGMDVRYADVDAYGLDQVEKVYRTVAGWKEDISKARSFEDLPVNAQ
                     NYVKMIEEASGVPVRWIGVGPERDATIRIKQDS"
BASE COUNT        12789 a        13093 c        11762 g        11481 t
ORIGIN      
        1 tgtttgtatc tgttcgtgga ttaccccagt ttgcaagagg agttggcggt tgttcattta
       61 aaggtgccga atctaaaccc caagcttgcc caacaagctg tggaattcgt gcagcggctc
      121 cgcaaagcgg acatgcgcaa gtcaccgtcc attagtgaga ctctagattg ggcaaatgca
      181 ctcgtagcgt tgaacgcgac gaatttggat aaggatgttt tggaagatac actttctgtt
      241 ctattgaagc acgaagcaga tttgcaaaaa gcaaagcggc agttgatgaa tccgccgcag
      301 aacccgaagc aacgtccgcc cgcggatgat tttggcaggt tcagcggaaa ctgatagtaa
      361 ccatactaca atgaagaatc cgccaaaaga cggccataat gggcttgccg cgcttctaaa
      421 aaccatattt ctattaatcg catttttgtt gtctctgtta ttcttgaaag aaaatcccga
      481 tttgggcatg gccttccagg aatggaaaca acacaagaga aaaaaggaaa tttccaaatg
      541 atgtaaaaga agcgctggtg catagagtgc cccatgccgc caaagcaatt cattctgatc
      601 tctctatttg ccatccttac cctttcgctg ggcgtcccat cggcagagcc tgtaaccgtt
      661 ccacagaaca tccccacggc aacggtcact gtgccgccat cgccgacgcc cacccgtaca
      721 gcgcttccca cccccattcc gtattacatc accgccacgg tttgggaaag ccaacctgca
      781 gcagccattc ttacctatca caagttcaag accaatcgcc catccactta tagcacagtc
      841 agcaggctgg attttgaaga ggaattagaa agcctgtatc gcagcggcta tgtgtcggta
      901 tcgcttgaaa aatggctggc aggtgacatt cgtgtgcctg tcgggaagcg cccggtcatc
      961 tttagcatgg atgatgtctt cttcagaaac cagatcgcat tgaccgctga agggactccc
     1021 gccaaagata caggccttgg cgtggcatgg gagttctaca aaaagcatcc tgagttcggg
     1081 tttcactggg ctctgttctc caacctggga gataaaccct acggagaggg aaccagcatt
     1141 gaacaaaaga tacaactggc gaatgtgatc gtgtggtgca tcgaacatga tgcaatgatc
     1201 tacaaccaca cgtttcggca tgtaaatctg aaaaacacaa aaggacttgg agtgaccgca
     1261 gaacttcgct ggaatgacga aaccctgcgc gaactgctac aactcgttca tcgcgcggac
     1321 ctgattccaa aactgcgaaa catggtggct ctccccggcg gcagggctcc ccgctgggaa
     1381 gattcacaaa ccgccctata tgggtacacc gacccagacg gaaggaaagt gcaggccatt
     1441 ttcaacattg actacatcac acgccccgat tttctcaagc cgccttatgc gcgaaagttc
     1501 aaccctgcaa acctgccgcg tatcgttgcc aatcttgagg caatcgagta tcttgtggag
     1561 aacaaagaga aataccctgc tgcaaaatcg tgcaggctgg gtccgctgga tcaaggtcaa
     1621 agcgctgatt ctgtttattt ggctgggcag gtttcggcag cggtgcgacg cggtcaatgt
     1681 ccgcatggag tgtacgtaac tgataaattt attttttcag cggaaacaga acaaaccagt
     1741 ttgattcaag aaattcaaaa cgataaccag ccttgatcgt ttgaaaacaa atcccacttt
     1801 agaggtaaaa agatgacaac ccaggaaacg ttcacccgtc cgaccacgat cgcagaaatg
     1861 caagagcgga tgaaaaattt tgcaacacca gaaggctgga aaaaaggcct cgcctataaa
     1921 cctgccccaa ccgatgtgtt catcgtaaca cctccaaaat gcggaaccac ctggatgcag
     1981 cagatcgttc atggactcag atcacggggc tcgatggatt tcgatgagat cacacgcgtc
     2041 atcccgtgga tcaacttggc acatgattca ggcattgata ttgatgcgcc gcaggtcgct
     2101 catccgcgcg catttaaaac gcacagcacc atggatgaag tgccgcaagg cggcaaatac
     2161 attgtggtac tgcgcgaacc cagggatgcc ctgctttctc attattattt ctttgaaggc
     2221 ttcttttttg aaaaaggctc cattgacatt gagacctttg caaaggaata ttacctgccc
     2281 gggcgggccg ttttcaaaca cattgcggca ttatgggaac gacggaaaga taaaaatgtt
     2341 ctgccgctat gctttgaaaa tatgaaagcg gatctaccgc ggaccgtgga acgggttgcg
     2401 gaattcatcg gcatcacact tgatgacgaa ttgaaagaga ttgttgtaag gcaggctgac
     2461 atcaaattca tgcaggcaca tgaagataaa tttgaagacc acatcatccg caaatcgcgc
     2521 agtgcagcca tgcgcctgcc catggacggc cagctttcca aggtgcggga tgggcaggtc
     2581 ggaggtgcga aactatcggt gcctgaaaac atcaagcagg aattggatgc catttgggaa
     2641 agtgagctta tgccaaagat cggcttgaat tcgtatgaag agttgaaaaa agaattaatg
     2701 cattgaaata cgtctgatga attggagtgc ttgaattatg gaatcccgaa tcttacaact
     2761 aatcgcagcc ttgcgcgcca gcggtgtgcg cgtttctttg gcggaatcag ctgaagcttt
     2821 ttcagctgtg gatttgatgg gtattcagga acgcgaagca tttcgattgt cattgcgcgc
     2881 cacgctcatc aaagatgtca aggatattcc caccttcgat aaattgttcc cgctgttttt
     2941 tggcagcgga cagccgccaa tgatgggcgg aaatccaacc gacgatcttt cacctgaaga
     3001 aattcaaatg ctggccgaag cactgcgtca gtttaaagaa agtctccgcc aaaacatgga
     3061 aaggctgatg aatggcgaac cgctgacaaa gtctgaattg gaagcgttgg gtcagcttgt
     3121 ggggctgaat caagctggcg acctcaacta tcagaattgg atggcgcaac ggatgatgcg
     3181 ggcgatggcc tttcctgaga tccagaaagc aatgcgcgag ctgatggaac agcttcagga
     3241 aatgggaatg aaccgcgagc gcattgaaca aatccgcaac atggttcaac aaaacatgca
     3301 ggggatgcaa aaccagatcg agcaatttgc aggccagcgc atcgcagaaa acctgagcga
     3361 aaagccgcgc ggtgaaaata tcgacaactt aatgaaccgt ccatttcagg cgttgtccga
     3421 tgctgacaag aagattctac aacgtgaagt gaaacggctg gcagcggcgc tgcgtacccg
     3481 cattgccttg cggcaaaaaa ggatgaaaag cgggcaaatg gacccaaaag ccaccatccg
     3541 cgcaaatttg aaatatcacg gtgtgccaat ggagatcaaa caccgtgaca aggttcgcaa
     3601 gccaaagatc gtggtgatct gcgatgtaag cacatccatg cgtttttgct ccgaactgat
     3661 gctcagtttc ctttttgcac tgcagggaca ggtacgcaaa acacatgcct ttgcattcat
     3721 tgaccatctc gaatccattt cagaggattt caacggggcg aacgccgacg aagccattca
     3781 atctgttctg tggcgtatgc ccagcggtca ctacaacacc gacctcggct gggcgttgga
     3841 caatttcaac agtgaacaca tggacacgtt gaacagtgga acaaccctca tcatcgtcgg
     3901 cgacggccgc aataactaca acgatccacg cattgacatt ttctccacaa tgacccgccg
     3961 cgctacacgc accatctggc taaatcctga gccgccagcc atgtggcacg gtgacagcga
     4021 tatggcgaaa tacgcccccc tgtgcagcaa tgttttaaag gtcggaaatt tacgggaact
     4081 ggcacaggcg gtggatgaat taatgacggg atagtttttg tggaaaacaa aatgaccgtc
     4141 caaacagcac tgaaaaaaac aagcaggtaa aaaattttag gagatgatca catgaaattc
     4201 accgccattt atatcgaacc gtatgccaac actgtcaccg caaatgtcat gtttgccgac
     4261 ccagagaccg accccgacca accttccgtt ctctctttct cacgtcccat cgaaccgggc
     4321 tttgaaaaca gctcttacta cttcgaaatc aacgaccagt cctatggaag ctatggcggt
     4381 ctggaaactg tgcaggtctc aagaaacagc cttgtagtcc gtgtgtcgcc ggaaaaggta
     4441 caagaattcg gtgaagagga ttttcgcgag atccaggttg actttcaagt ggatgacgag
     4501 acttacggaa atgtcctcgc ccagctaaaa cgcatcttcg aaggatttga tatcctccaa
     4561 atccaataaa caaaaaatct caaattggag gatctgggtt tcaggtcctc caaaaggacc
     4621 acctgcatgc cctggaaaat ctcctatctt gaagacacca agatcataaa aacgatctac
     4681 accgaaccca tcacactgga tgagttaaaa gaatgtgcaa tggcaaattt ctccatggct
     4741 gccgaaaagc aaacggggtt attccttggc gattgttcca gtttcactca acaaggaagc
     4801 accatggata tttacgagtt gggaaaattt cttgaaagca tcagcctcga gttgcgcatc
     4861 catatcaagg aagccctgat cgaaccgtca aacatccaac gcgtatccag cgatctccgt
     4921 tttttcgaaa cagtcaccaa taatcgaatg atcaaagccc gaatttttca agatacagaa
     4981 agcgcgcttg aatggctact taattaaatc gaagtttttc agtccactct ccgccaaact
     5041 acgtagtgag atttacgaga aaatacgttg acaaatttgc gggtaatacg aatgacattt
     5101 tgaatacttg aatttagaat taatctggat gtatttcaaa atgccaatct cctacgatat
     5161 ttcaaacacc ttcaaccttt tagtttatat ctgcacagat tcctggaccg gaactgcttt
     5221 cttcaagacc gccgataagg tccccgaaga tccgcgctac tgttcaaaca tgaagatcat
     5281 catcgatatc ttttcggctg aaatcgaaac ctccctttcg gatatgcacc tggccataga
     5341 aaaaaacagg cagctcaaac aaaaaggcca aaaacttggg caaaccgccg ttttcacaaa
     5401 aagcacaagc ctgatgttcc tggccgatgc cttcaaactc atgtcaccgg aagcaccgtc
     5461 caacttcggc gttttcaata ataaacagga tgtcgtgcgc tggctgggct tcaccgacgc
     5521 agaaaaagag gtcatccgct tttgggaagg cgcaaaaaca agcccgccag aaaaagttcc
     5581 ataaggctgg agtttgaaaa gcagggggtc tgcggtacac ttaaatgcat gtccaatcaa
     5641 aacatcgata ccatcatcat cggcgctggc cctgcagggc tggcggttgg agcctgcctc
     5701 aaacaggcca accattcagc cattattctt gaacaagata acgcggtggg cccggtctgg
     5761 aggcgtcatt atgaccgact gcatttgcac accgataaaa agaattctga actgccattt
     5821 gcgccattcc caaaggaata tccccgctac ctctcacgcg atcaacttgt ggattatctt
     5881 gaagcctacg caaaaaaatt tgagctaaac attctcttca accagcaggt gacatcggcc
     5941 aggagaatta atgacagatg ggaaatccag acaaacaatg cacaatacca cgcatcacaa
     6001 ctggttatcg ccaccggtta tgcgcgtgaa ccatacatcc ctgattggag cgggaaagat
     6061 ggtttcaagg gaaagatcat ccacagttcg gaatataaaa acggatctga atttaaaggg
     6121 cagagggttc tcgtcattgg gtttggcaac tcgggtggag aaatcgccat tgacctgtac
     6181 gagcatggcg ccagggcagg gattgcagtt cgaaacgcag tgaacgttat ccccaaggaa
     6241 ttggctggaa ttcccattct ctccatcggc atcgtgcaaa atttgatgcc tgtctggctt
     6301 gccgatgcaa tcaatgcacc gatcttgcgg gccgtcatcg gggatatcac ccaatatggc
     6361 ttgcgcaaac tgccctatgg ccccgccacc cagatcagaa acgacaaaca cattcccttg
     6421 atcgatatcg gcaccatgaa attgattcgc gcagggcaca tcgacgttta ttcaggggtg
     6481 gagaatttcg caggaaacag aatcaaattt acggatggaa aagaagcgga ttttgattcc
     6541 atcattctgg ctacaggcta tcaacccagg gtcaactcct ttttgcagga acaatcggta
     6601 tatgacgaaa atggcacgcc catctccaca gggcgtgagg ctggtgtgac aggcctgtat
     6661 ttttgcgggt attatgtatc acccactgga atgctccgcg agatcgcaat ggaggccagg
     6721 cagattagtg ctgccattgc taggaaacgg tccctgtgaa aaagttacgg ccgattaatt
     6781 tcgatttcat gccaattttc tgcaggcaga agaaatcagg ttttaatggt ctcacctcta
     6841 tggcttgcgc cgtaaacttt ttaattgtat gattgaggag aaatttcacc atactgggaa
     6901 gatatcatgc ccaaaataat gatcgtagat gatgacccgc aggtcataac gctattaaaa
     6961 agataccttt cagtggaagg ctatgaagtg attgccacca ctcacagtgc caaggcagtt
     7021 cctctggcaa ataccgagca tccagatctg ttcatccttg atcttatgat gccgcctcca
     7081 gatggtttca cgctttgcag tatgctgaga gctgatccag ctttcagact gacacctatt
     7141 ttgatcatca ccgccatggc cgatagcaac agcaaggcaa cttcatttgg cgcgaatgat
     7201 tatctggcga aaccatttaa tcttgatgaa ctttccctca ggatatcaac cctgttgaat
     7261 ggctgagatt tgacacaata attgcaagtg gtatttgtat tattatcgtt attttacagg
     7321 tattcataag tccttttaaa agcttttcgg gcaatgtgga attcgatctg ctgaaaaact
     7381 ccaatcacaa ggaagaatga aatgtcaaaa atattaataa tcgatgatga tatggaaacc
     7441 acaaagcttt tggaaagcct ggtaaaaatg aacgggcact cgcccacatc gatcaataac
     7501 agtgccgatg caattgaaac tgccaattcc atcattcccg atctaattct tttggatatt
     7561 atgatgccca gaatcaatgg catccagttg tgcaaaatgt tccagtcgac ccagaccctc
     7621 aaacacatcc ctatcatcat tgtctcggcg ttgaacgata ttggcagcaa aaaggacgca
     7681 tttaacgcag gggccaaaga ctttattaca aaacccttcc tgcccaagga attagcccaa
     7741 aaaataaacg cactgacctg aagcaacagc ttacaaattt gcgaggaaga ccgcaactgc
     7801 caattttcat cgcaggggcg gtctttcggt taaatatgtc taaggtataa tattaaaaat
     7861 attattcagg aaaattcatc caaatcagtc aggaaaatgt catgaccaaa attttgattg
     7921 tagatgacga taaacaggca acggacctgt tgcaaaaggt tctaaccgtg gagggatacg
     7981 agacaacagc agtgaatgac agttccaaag ccgtgcagat cgccgattca gtaaacccac
     8041 acttgattat tctcgacctg atgatgccag accctgacgg cttcaaagta tgcagaatgc
     8101 tccgccagac accgcgcttc atcttcaccc ccatcatcat tgtcaccgca ctggatgaca
     8161 gcgacagcaa agtggtcgct tttggcgcag gagctgacga ctacctcaca aagccatttc
     8221 acattgatga actatcccaa cgggtaaagg aattactcgg aagaaccgaa taatcagcaa
     8281 tacaaaaaac atctattttt caaggcgtgc aaatttattg cacgcctttc ttttattcac
     8341 ctcaccttga ctccttttcg gttttgaata taatttctgt gaccattttt actaaattag
     8401 tcacagaaag gcaatcccat gccaaaagca ttttcagaac aagaaaaaga caccatccgc
     8461 ttgcagatgc gggaaaaggg aaaaaagctc tttgagaaac acggcctcaa aaagaccagt
     8521 gtggatgaat tgaccgaagc cgtgggcatt tcaaaaggcg ccttttatct attttatgag
     8581 tcaaaagagg aattgttcct tgaaattctg gaacaattcg aaaatgaaat tcagacccaa
     8641 attcttgagt tcacgatcgc gccaaaagca aacgcgcaga aaaatgtcag cgagatgctc
     8701 aagggactat tgttgacatg ggatgcctac cccctgctga agaatttcag caaaaccgat
     8761 tttgattacc ttgtcaggaa aattccatcc gaacgggtga tgcaacacgc cagcaacgac
     8821 gaggcattca caaccgagtt cataaaaaag atcaaacgcg aaggcattaa cgtcaaggcg
     8881 cctccgcggg ttgtaagcaa cctcatcaag agtctgttct ttatggggct gcatcgtgac
     8941 gatatgggtg aaacagccta tcaggaaacc attggtgtgc tgattgatct ggtggctggg
     9001 tatattgctg gagaataaaa tggaatacgt aatcgagaca aataatttat ccaagacata
     9061 tggcagcata aacgctgtaa acagcgtcaa tttacgggtt aagcaaggcg agatatatgg
     9121 cttccttggc ctcaacggtg caggcaaaac caccaccatc cgcatgcttt tggggatgat
     9181 tcgcccaagt gaaggaaatg taaaagtgct gggtcaggtt ttaggtccag gtagaagcgg
     9241 accatgggcg caggtgggac atctcgtgga aagtccatct gcttaccccg atctgaccgt
     9301 ccgcgaaaat ctggatatcg cccgcagatt gcatggattg aaaaattcca acgcagtgga
     9361 tgatgtgatg gaaagtcttt ccatcgcttc ttattccaaa cggaaagctg gtgcgctttc
     9421 attggggaac cttcaacgcc ttgggctggc acgcgcaatg atccatcatc cgagtctgct
     9481 gatccttgat gaacccgcca atgggcttga ccctgctggc gtggttgaaa tccgcgagtt
     9541 gatcgccaat ctggcgaaaa acggcgtgac cgttttcatg tccagccata tcctcactga
     9601 agtggataaa ctcgccacac gaatcggaat tatccataaa gggaaaatga tcgaagacct
     9661 cgacgcgaac aaacttgagc aattccgcgc gagacgcttg aagatcaagg tccgtgattt
     9721 tgcaagagcg catgcagtgc tgaccaggga aaactattcg gcaacgatca ctgaagattc
     9781 tattttcgtc agcgatacgc gggcactcga aacaccggag aaaatcgcat gcttgctggt
     9841 caatgctgac acgccaccca cccaccttgc agtggaacag caaaaccttg aagaacattt
     9901 tatgcaattg acaggaagta caaaatgaat gcttatcttg ctgctttaca aaccgagagt
     9961 ctcaaggcat ttcgttcgaa agtgccgcta ttcaccctgt tgggattttt gatcgcgccc
    10021 ttaatcggcg gcctgttcat ggtcatcttg aaggatcccg aagccgcaaa atccatgggg
    10081 ttgatcggca caaaagcaca attgtccgca ggtgtggcag actggccgac atttttcaat
    10141 ctgcttgcac aagcaacggc cattggcggc gcgatcattt ttgcaattgt caccacctgg
    10201 gtgtttggcc gtgaattttc agatcatact gtaaaggagt tgctctcatt gcccacatcg
    10261 cgcgaggcga tcatcgcggc aaaattcact gtgatcggtt tttggacggc ggccctaacc
    10321 ctgcttagtt acgggcttgg tttgatcatt gggaatgctg ttgccatccc tggctggaca
    10381 aatgaactct tccgctcggc aaccatcgat attcttggcg cgggcatcct gacgatcctg
    10441 ctgttaccgt ttgtagcttt catcgccagc atcgggcgcg gatttatggc cgcttttggc
    10501 tgggcgatct ttaccgttgc cattgcacag gtcgctgtcg taatgggatg gggtgactgg
    10561 cttccgtggt cggtgccagc ccttttcagc ggagcgattg gcgcacattc tgaaacactt
    10621 ggaatgcaca gctatctcat tgtggcgatt gcaagcgcgg tcggtttttt catcacattt
    10681 tattggtggc gcaacgcgga ccaggcaaag tgaaccaatg cacgtccttg tttgggtctt
    10741 ttgctggtta agccagcaac ttctcgtaca ccttcaataa tttcttctgc gattcgtgcc
    10801 aggtgaattt ggtcaacgca cgttcggagg cagatcgggc caggcgtttg gataattcaa
    10861 aatcattcag caaggtgatg gtctgcctgg caaggtcttc aggatcgctt ggagaaaata
    10921 acaacgcatc catatcttcg cggacaagct cacgcacaat gggcatattc gaagccaaca
    10981 acggacgttc cgccgccata tactcgagga ctttgatcgg gcacgccccc tgggtgacat
    11041 ttctgtcatt caaaccgagc ggggcaacac agatatctgc actggcaata agcgcaggga
    11101 tttcatggtg cggtacggca ggctgtacgg ttacatgttc ttcgatgcca agttttctaa
    11161 tttgcttcgc aagcaccttg cgctggcggg aacgtccgcg cccgacgatt tttaagtgaa
    11221 caggttgttt ttcgaggatc tttggcagcg cttttacgac gatatccaat ccctgccaat
    11281 cggcaagagt accgatataa agcagcacgg gaatccttcc atcgcgggca ggcagggatg
    11341 acggggaaaa gtccgaaggg ctgactccgt ttgggatgac agtgaccagt ttacggtcaa
    11401 gcccgagcga ggcgatgtaa tcgcgagtca cattcgaagg acagatgatc gcgtcgctca
    11461 ggtgcagagt cgcaatttcc tgttctttta ttttcaagag caattcaggg tcgagtccgg
    11521 ggtagtgata tttgagctcg atggagggca ggccattcac ttcaaaaagt gttttgtagc
    11581 caagcctttt tttattttgc gcaatggaga ggccatccca tatatttcga aaatggacaa
    11641 catcgtaccc ctgatgcagt gccagatgtg ccgcaaccga cctgccaaag tgcatggccc
    11701 gggcaaggaa gttctgcgag aggtcctgcg aaatgcgggt gagctgcgcg ccctcaatag
    11761 tatcttcggt gggtagaagc ccatcgttgg gcgtgataag atgggcactg tgccccccat
    11821 tgaccagccc gcgcaaattg tgcaaaatat gcgtggatgc gcctttcggg cttggtacaa
    11881 tatcgaaagc ggtgtagaga atcttggcca ttgctgaaat tatatcgctt tcagcaaaaa
    11941 agaattttta gcaatatcct gtaagccact cggaggaatg tttcatgcca gcgttatgtg
    12001 ttttaggcgt agtcggcctt gttgtcgtgt cgctcttcta ttatctattg aatacagccc
    12061 gaaaaaaggt taaggccccc accaacattg cagggaaaaa agagtacgcc cctgtctatg
    12121 tgtccattgc cgacaagccc gattcgatca tgcgcggcat ggagcgattt aaagccgagg
    12181 tgcaaaagac cgaaaccgct ggtgacaagt ggcgttggat tccccttatg attttcttcg
    12241 tcggcctcgg catgatggct gtggacggtg tgcttctgct gtttgggtat ttttcgctgg
    12301 ttttcacttc aggcgggtta ttgttgtgga tcgccgcgtt catcatggcg cgcagtttgc
    12361 gcagaagcga tacgctcgat tttccgccgc gttataaagg cacaaaggac attttataca
    12421 ccctgcgtga cgacttgaaa cccggcagca cctttcttgg tcatctcgac ctgacagggg
    12481 caatgctgaa aaacaaggtg gcccgcgaaa cgcatgatac gcagaaccgc accactcatc
    12541 atttccgcga tgaatggctt tcgttgaaag caaaactata tgacggcaac gttctgcgca
    12601 tttcggcgat acaaaaaacc aaaaagcgcg cctcctattg gaagcgcagc cgaatcagcg
    12661 gaaaatcaaa gttaaagccc gaaaaatata aaggcacatc acatgatctg aaggtacgta
    12721 ttgtggtcaa cccggaagcg taccagatcg tccccaacaa ggagtttcgc ccgggcgtga
    12781 acatcggcaa gtacaccatt tctcaattaa gcacagacgg cggcatgatc aacatggttg
    12841 ccagctcccc ctttgatgaa gtggacaacg aacagatcct tgccatttta aaatcaactt
    12901 attctctttt acaacggaag gctgcataaa tgagtcgcaa aatcataccc tttatcattg
    12961 cattggttct ctcctgtctt gtcatggttg tagcgggcgt gttcgctttc agcggcgcag
    13021 tgaccgcgga aaaattcggc agtgacccgg tctggtccag cgaataccca accgctgaaa
    13081 gcatgaaaat catcgacctg accggggatg gtcaggatga gcttttcatt caaaacgcct
    13141 ttggcataag actttacgac cccaacggta atgtcatcag tgacttcacc tacgataaac
    13201 ccaaaaccac attgggcgat gttaacggtg atggcgtgga ggatattgtc gtttatcaca
    13261 gcggaaactt tttttcggta gacaccatta tcaacggcca gcaaaagacc attgccagcg
    13321 aattgcctgt ttttgtccct gcacgcgtgg cattgatccg ttttgagggc ggctcccaga
    13381 tcgttttagg agatgtctcc gggcagatcg cctccatcac caccgatgga aagatcgtgt
    13441 gggaatctga ctttgcgagt gaggaaatcc gaggcatgga tgatgcaaag atcggcggcc
    13501 agacctatgc cgccatcgct tcgcacgacg ggacagttgg tgtcttcgct gcaaacggat
    13561 cagccgtttg gcagttggaa caggaacagc ttcgccgcat gcgtgccttc gatttgaacg
    13621 ccgatggcaa cagcgaaatc atcactggcg gcgagttagg cgccttctac atttacagtg
    13681 ccgtggatgg ctcaaccatc ttcagcaaat cactcggaca ggcggtcagc gaagtacgtg
    13741 aagtggaact aaatggcgac ccatcctcac gcgagatcat tgtcggcggt aaagacggcg
    13801 gcatttgggc attttcctat aacggcacaa ccgccactca aatgtggacc gcctctctgt
    13861 ctgacaaggt caccgaaatt tcaggcctcg acatcaatga agacggcaaa gaagaagccg
    13921 tcatcggtga cgattcaggc aaggtggctg ttttcaccga aaatggcaca cgcaacaacc
    13981 tgccctctca taacaccggc atcacccgca ttgacatcgg caagctgggc aatgaacggt
    14041 atgtagtgat cgcagatggc agtcatgtcc aaaccaacaa ggtcaatttc aactccatcc
    14101 ccgggttcca gttcaccccg ctgatcgtcg ggctggtcgt ttcagcgatc atgctggtca
    14161 ttgccggggt cctcgcctcc attccgccga aacccgaatt gaaggtctcc ttccaggata
    14221 aaagcaagga atctctcgaa gcccagcgcc gcatgttgaa agaatccatt gcggatgtgg
    14281 aacgtttgcg caaggcaggc gaagtgacag gtgatgccta cctcgcaagg ttgaaacgtc
    14341 tgcgcagcga cttggctgag aatgaaacag cctacaagac agcaggttac accattaaag
    14401 ttgaaacgtt ccaatgcccc cactgcggcg gcacccttga actcggcatg gacaaatgcg
    14461 agtactgcgg gcatgtgatt ctttcgtaaa aaatattttc agtatcacat ttaaaaacat
    14521 gacgcatccc gaaccattcg aggatgcgtc atgttttata ttacaaaaaa caattcaggg
    14581 attaatcttc gtctgttttc caggcagtta gtttgttcag ggtagcaact tcatcatcca
    14641 tcaagtggac attcaatgcc ccgaggtttt cctgcaactg ttcaatggtg gtcgccccaa
    14701 tgatggggct ggtgatcacc gcatcggaaa gcatccatgc cagtgacacc tgtgcaatcg
    14761 aggccttgtg gcgcaaaccg atcgattcca tttcatacaa cagggaaaaa tttttctcgg
    14821 tcattgcccg tttgcggcct tcggcccgct tgctttcggg caaaggctca ttgcggcgat
    14881 atttgcccgt caaaaagccg cctgccaggg ggctgtatgg gatcacggca atctcatagg
    14941 ttttgcagac atcccgcagt tcccgctcaa actcatcgcg atagatcaga ttgtagtgag
    15001 gttgaaggct gtcgtatcga gccagattga gtttatctga aacccacaga gcctgcatca
    15061 actcccaggc tgcataattc gaagccccaa cataacggac ttttccctgc cgtacaaggt
    15121 catcaaaagc ccgcatggtc tcttcgatcg gagtcgattc atccgtccag tgtgattggt
    15181 acacgtcaat atagtccgtc tgcaaacggc gcaacgaccc ctctacggca ttcataatat
    15241 gaaccctgct cagtccttca ttattcgggc catttcccat ctcaccacgt acttttgtgg
    15301 caattacgat ctggtcacgc ggaatcttgt tttgcttcat ccatgtgccg atgtacgcct
    15361 cagaaatacc acccgggttt ccatccaccc atttggaata aatatctgct gtgtcgaaga
    15421 aattgatccc cgcctcaaaa gtggccgaaa gaattttgtg cgattctgcc tcaccgagcg
    15481 accagccaaa ttgcatggtc cccatgcaca aaggagagac cttgagaccc gttttgccaa
    15541 atttcctata ctccatgttt gcctccatta ttgtgaatta tacgacgatg cagtacaccc
    15601 ggggctgcca tatcaataag ggagtgacct cttcttttcc ctgctttttt gtaagcggca
    15661 aaccttctta aaaataaagc gcacctcgat cgtatcgagg tgcgccatct tcaaaagtcg
    15721 gcttcaaaaa ctattgaacc gagaactgct gttcacccac cttcgcgccg ttcaggtaaa
    15781 tttcgacctt gtattgtgcg gcggggaaac cgccatccgt gctttcgata ttggcataga
    15841 tggtggcttc atcagtatag gtgtaatcag tggtcgtgaa cgcttcattc ggatcctgac
    15901 catccacagt caacgcatac cacttcacct gaaactgtgc gccttcgtca ataccgttca
    15961 catcgaaaaa cacatagatg acatcggttg gcgcaaaagt ggtggtcttg ttattgcctt
    16021 cttcatcatt tgccatgtac acattggaaa cgcccgcagg ggcggtagac ccacaagcca
    16081 aaacagacgc aaacaacaca gctacaacaa gcaaaagcga gagatttttg agtttcattt
    16141 taaacattct cctttggtta atttctggca ttataaaaca agagcggcaa cgnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttaaac attctccttt ggttaatttc
    16321 tggcattata aaacaagagc ggcaacgagt attcccctat tgggtctatt cgatttcgat
    16381 ataaagccgt ttgttgatcg cgcctttgct cttgtaatcc acaactttta cgcggcccac
    16441 ttcagcagta ttcgccacat gtgtgccgcc atccgcctgc aggtcaaggc cgacgatctc
    16501 caccgttcga acctgcaaaa taccctcggg aagaaggttg atcttggtgc ggatcaggtc
    16561 tgggatttgg aaggcttctt cacgcggcag aatcttcacc cgaatatcgc gtctattctg
    16621 gacctcgaga ttgaccgctg cttcgatatc acggaccagc tctccacgca tggtttcaaa
    16681 ttcaaaatcc atgcggccct tgaggggatc catatcgccg ccggtgacct tggcgccata
    16741 atcacggaaa accgtgccgc acaggatgtg caatgctgta tgggtgcgca tcaactgatg
    16801 tcgccgcacc cagtccaggg tccccgcgca gcgtgaagcg gaattggaag tcggcaaagg
    16861 ttcggttccg ccgagaaaat gcaggacgtc atcgccctgt tttttcacct tctccaccgg
    16921 atagacaaca cccgccactt ccagtgtgcc aaaatcgcag ggctgaccgc ctccaccggg
    16981 ataaaacgcg gaacggtcaa gaattacgga acggttttca gaatccaccg acagaatggt
    17041 ggctgaaaac tctttgaggt acgcatcggt ttgatagagg agttcggtca tgcgcaaatt
    17101 atagccgaag gcctagaaaa ttagcgttag attagaagtt aattacctgc ttgactttgc
    17161 gactctacct attgtatagt aatcaacggc aaaacagata gcactgcctg cgggtcgtgc
    17221 tttttatttg gcaaaacccc attcttaaaa cataattttt actgcaagga gaggcaatga
    17281 tcaaagttag tggtctgacc aaggactacg gcgcacgccg cgccatcaat aacctgaaat
    17341 tcgatgccca gcagggagag atcgtgggct tccttggccc caacggcgcc gggaaaacca
    17401 ccaccatgcg cattctgact ggctacatgc cccccacaga cggcgaagcc attgtggccg
    17461 ggtacaacgt ggtggaggaa tcgttggagg ttcgcaagcg ggttggctac ctgcccgaaa
    17521 cggttccgct ctacaccgat atgaccgcct tcgattattt gaagttcatg ggcgagcttc
    17581 gcaagatccc caacatagat gaccgcgtgg aagaagtgct ggatatggtc ggtctgatcg
    17641 atcgtgccga tagctacatt ggcaacctct caaaaggaat gcgacaacgt gtgggtctgg
    17701 ctcaggcgtt gatccaccgt cctgaggtgc tgatcctcga tgaaccgacc atcggccttg
    17761 accccggtca ggtggtggaa gttcgcgagt tgatccgcga gatcggcaag gaacgcaccg
    17821 ttctgctatc aacccacctg ctgcacgaag cgcaaaatat ctgcgaccgt gttttgatca
    17881 ttaacaaggg caagatcgtc accgaagaca ccacccaaaa cctgcaatcg cgtttgatcg
    17941 gcggtgagcg tgtggtggtc cgtgttcgcg gcgaggccga tgccaatctg gtcaacctga
    18001 tcaaggctgt gaaaggtgtg gagcacgccg ttctgaccaa ggacaattcg gtggagtttg
    18061 actttgtctc cggcaaagat gcccgccccg aagtagccaa acaggtgatc aactccggtt
    18121 acgaccttct tgaattacac ccactcggta tgagcctcga agaaatcttc cttgagctca
    18181 ccggcagcga aacaaagaac aattaaagag ggattccatg agaaatatct ggaccatcgc
    18241 caaacgcgaa tacgataact acttcaacag cccgctggct tatgtggtgg cctttgccat
    18301 cgtcctgccc atcggcattt attttgcatt gatcatgttc gtcagcgccc agcaaaccat
    18361 gatgggcggc cccgcccctg acagcactcc catcaactgg ctgttcgtct ttttgctgat
    18421 cttcctcagc ccggcattga ccatgcgcct gctttcagat gaagcccgca tgggcacact
    18481 cgaattgctg ctcaccgctc ccgtccgcga ttttgaactc gtggcaggga aatttctggg
    18541 cgccttcatg ttcgtgctga ccattgccct gttcaccctg atcttcccga tcattattga
    18601 taactacgtc acccccggcg tggattggat gatcattctt tcctcctaca tcggcctgat
    18661 cctcatcagc gccgccctgc tgtcgcttgg cacaggcatc tcggccattt tcacaaacca
    18721 gttcgccgcg ttcttcgtaa ccttcggcct gttcttcttc ctgtggttca tggtcaacct
    18781 gcccgccagt tacctgcagc aaggcggtga tgtttacaac tacctgagcc tgtcgcaaca
    18841 tttctcatcc atgaaccgtg gcagcatcgc tcttggcgat attgtttact acatcagtct
    18901 cattgctctg ggcctgtttg ccggtaccac tgcaattgaa attcggagat ggcgctaatg
    18961 attgacaata aaaacaactc tcctgcgaaa tatgctttca ttgggctcat cgtcgccggg
    19021 ctggcctgtg tttcttcagg cctcactggc attgccaaag gcatggtatc gcagcaaatc
    19081 tttaccctgg aagaaaccct caccaaaaac attgacctgg ccttcaacat cagcctcgca
    19141 ttaatcgtca ttggattggc ggcctacgcc atcatggtcc ctgaccagat ccgccgcttc
    19201 cttaccggac gccaggcccg ctacggttcc aattcgctga tcctcatcgt ggcatttctc
    19261 ggcatcctca ttacggccaa tgtgctggtc ttcagaaatc ctgatttcct caaatgggat
    19321 atgaccgagg ataaatccaa cacactcgcc cctgaaacac tgcaggccct tgaaaccctg
    19381 cccggtgacg tgactgccac agcctactac agcgtgaatc tcgataacgc cagtgctgaa
    19441 gaactgctca ccaaatttaa gaacaacagc aatggcaagt tcacctacaa attcattgac
    19501 cctgaccagg accccgtttc agcccgtgaa gctggcataa ccggcgacgg caagatcctg
    19561 cttcaaatgg gcgaaaccaa ggaaattgtt tccttcgcca gcgaatcaga attgacacag
    19621 gctctcatcc gcctcattaa ccccggtgaa cgcacggtgt atttcctgca aggacatggc
    19681 gaaccctctc ttgaagcggg cggcggccaa ctgagcttct ccattgcaaa atccactttg
    19741 gaatcaaaga attacacggt cagcccgtta aacctgatcg ccacgaacaa gattcctgaa
    19801 gatgccctcg ccatcatcat tgcgggtccg ttgaaaccga tctccagcgg cgaaatggtt
    19861 ctgctcaagg actatatgga agcaggcggt tccctcgtag tcatggaaga ccccaccatc
    19921 gtcactgaat ttggcgacac ctttgacccg ctcgccaaat acctcgactc agactggggc
    19981 atcaaactga atgaagatat ggtgatcgac ctcgtcaaca cccagaaccc gttgcaggct
    20041 gtttcctcac agatcgtgga tcaccccatc acacagaacc tgaccgagaa ttacatcgtc
    20101 atcctgccgc aggcacgcag catcagcgta tcacaggaac cgatcgaaaa cgtatccacc
    20161 acccccatta ttctgacgac cgaccaatcg tggggtgaaa ccgaaatgtc tgccgagacc
    20221 cagcctgaat tcagccctga aaaggatacc cccggtccgc tgaaccttgc tgcagtggca
    20281 gaaaactccg ccaccggcgg gcgtgtggtc gtattcggga attccatctt tgccaccgac
    20341 gagatcttcg atgcctacgg caacggcaac atgttcatca attcagtgga ttgggccgca
    20401 gaacaggaag acctgatcaa cattactccg cgtgaacaga cccagcgcat gttcacaccg
    20461 cccaccaatg gtgtgttcat catcatgatc atcatttcca tcttcgtgct gcctggcctg
    20521 atcgtgttct ccggtgtttc ctcctggatc gcacgccgca agaaggggta gaacatgcct
    20581 cgaaaagaaa caaaacccac ggcaaaggca tctccgcgcc gaaaaaagac gaacgttgtc
    20641 acggagtcaa cctttacacg ggaatccact ggcacgcaaa tccagaagcc tgtgattggc
    20701 atcggcacgt ggatcgccct tgtcctgttc gctgggctgg ttacctttgc aatcttcctg
    20761 aacaaaaagg aagaaaccgc agtggaggca acgcccactt caggaacggt acccctgttc
    20821 cctgccacag aagggactcc ctccagcatc gaagtaaagc ctgccgaagg tgaagcggtt
    20881 cgggtcttgc gaaacgagga aaatgtctgg gtgctggaac tgcccttcga aaccgaggca
    20941 gacccggggt tgtctgaagc ggcggcgacg caagtttccg cacttcagga gatcagcact
    21001 gttcaaggtg acctggcaga tttcggcttg gaaaatcccg cctttgttat tacggtgggg
    21061 tttgccagcg gaaaaaccca cactctggaa atcggcgata tgaccccatc gaaccgcggc
    21121 tattatgtgc gtgtggatga ggaccgcctg gtagtggttg ctgtaagcgg attggatgct
    21181 ttgctccagt tgcagttctc tccgccttat ttgaacacgc caacgccaac cgccacgaca
    21241 ccgccgccaa cagagacgcc cacccctgca ccgccaacgg aaacccctgc ggcttccgta
    21301 accccaacgc cttaatgggg taattacaac tgctgaacgc gaacgtccgg aaatactccg
    21361 gacgttcgta atacaaaagc attgcaaagc atctgaaaac agggcaggta ttgattttca
    21421 ggttaaaaaa gtgtattctg atttagcagt ttgctggtaa atataattga aagttggata
    21481 tgtaaatgga tgtagagaag attttgattg tagatgacga agaagagttt tcagctatcg
    21541 cacggctcgt ggaattgggc cgccaaaaat catatgtaac gttggatgac atcctgcatt
    21601 tcttccctga agctgaacag gatgtggaac agttggaaga ggctttttcg gctttgttga
    21661 gcgctggtat cccttttatg gaagatacca tcacccccga gccgaccgaa gatgaattag
    21721 ccgctgttga gcaaacgacc gaaacggaag atctacccga cccctcgctt gacgattatc
    21781 tggcaaacat cgacacagac gatacgattg gtctatacct caaagaggtc agccgcgtac
    21841 cgttgttgac cgcagtggaa gaggtgcaac tggcccaacg catcgagcgt ggccgcgccg
    21901 cccgcgaaga actggcccat ggaaacgtga ccagcaagcg ccgtcttgaa cttcgccatt
    21961 ccatcgaaga cggttgggca ggccgtgaac atctcatcac tgcgaactcg cgtttggtga
    22021 tttcggtcgc gaaaaaatac atgggacgtg gtgttccctt ccttgatctg attcaggaag
    22081 gcaatatcgg tctgatccgc gccacgaaga aattcgatta ccgccgcgga cacaagttct
    22141 ccacctatgc cacctggtgg atccgtcagg ctgtgacccg cgccattgcc gatcagggac
    22201 gcaccatccg tgtgcctgtg catatgggcg accagatcaa caaactcctg cgtgtgcagc
    22261 atcaactcac ccaacgcctt gggcgcgaac caagcgtgga tgaattggcc gtagccctgg
    22321 atgtgccgcc caagaaagtg gaaaacatga ttcaggtggc gcgccgtcca ctttcgctgg
    22381 aaacgccaac cgatgacgaa gaagattcgg tgctcggcga cttcatcgaa gacgatgagg
    22441 ctcccccgcc cgatgaaacc gccacttaca acctgctgcg cgagcatctc ggcgaagtgt
    22501 tgaacggtct tccgccgcgt gaagtgcgta ttttgcaatt gcgctatggt ctgctcgacg
    22561 ggcaggccta caccctcgaa gaagtgggcc gcaaaatggg cgtcacccgc gagcgtgtaa
    22621 ggcagatcga agcgcaggcc ctcagccgcc tgcgtcaccc ctccatccgc aggaaactgc
    22681 gcgactattt aggcgaataa attacaaagc ccttgagaga tcaagggctt ttatattatc
    22741 tgctacaata ccgaaaccca gaccctgcgc ccatgaaaat caacattgat ctttcacgaa
    22801 cagccatcac tgccatattg atctcgttgg cgctggtcta tcccctgctc tggctgaaga
    22861 tgctcgcgga ccccacccaa cacacaggcg cggactttat tgccttctac gcggcaggac
    22921 gcattgcaga ccaggaaggc ccttctcatg cctatgacct gattttgcaa aaaaaatatc
    22981 aagagggggt tgttcagcgt gagatcgaat tgcaggaaac ctttccatat attcacccgc
    23041 catttgtgat tccgcttgcg cagatcgttt caaacacaga ttatctaaaa tcctttcaac
    23101 gctgggcact gttgatgctg ctcctcttca cagctgggat tccgtttttg attgcgcccg
    23161 ccagaaaatt tttttcacaa cctcaaaccc tgatctttgc cgctggcatc ttccttttct
    23221 ttcccatctt tcaaagcctc ctgctcgggc aggataatgc gatcatcttt ctggggctca
    23281 gcttattaat gtggggcacc tacgccaaaa aggattgggc cgcagggctg gggttatctc
    23341 ttgccacagt gcgtccgcat tttgcgtttt ttctgttgct tcccttcctt tttcaacgcc
    23401 gcgctatttt tgggtggttc ctgcttttcg tcttcgcgtt gacattattc agtttcgggg
    23461 ttgtaggcac ggacggtttg aaaggctttc tgcacatcct cacggtcagc ggcagcggcg
    23521 agggctaccg caccagcgaa gagaacatgg tcaacctcat tggccttttg cgaaggcttt
    23581 tcccatttct ctccgccgcg ctgatccgaa gcgcgggctg ggcattttac ggattggcat
    23641 ttttttcaac aggagtttat ttctggcgaa atcacacaag cccttcggga gaagagatca
    23701 gtcttgcagt tgtagcggcg cttttctttt cacctcacac ccacgcgcag gatctggttt
    23761 tgctcatcgc accgatcatg gcggtgattc tcatcctgct tgaaagacaa cgcattcaac
    23821 ccacacaggc ggtattaatt ccgctggcca tatcacttct gcttttattc agttattatt
    23881 cgcccatcct cacccattcc ataccctacc tgcttatggc tgggctgttc ttcgccgtaa
    23941 aacaatacaa agccctcccc gcaaaaccat gacccgaacc gagcgttttt tcatcaccat
    24001 cgtattttta ttggtcgcca ccatcgccat caatccacct gtggataatg acatgtggtg
    24061 gcatttacgc gctggcgacg aaatgtggca tcagggccgc atcctcctca ccgaccaatt
    24121 ttcatacacc aaacaaggcg cagactgggt caatgcattc tgggtttcag accttgccct
    24181 ctacggcctg tggcaggtcg gcggattttt cgccatcaca ctgaccaccg ccctgctggc
    24241 aattttcact ttgtggatca ttttcaagcg gatgacgaac gcgatcattc cgcgcggatt
    24301 gctggtcatt ctcgcgatga tggggataat cgcaaactgg actccccgtc cgcagttgct
    24361 atcctttttg ttactggcat ggctggattc atttttatat gagcacatcc acaccaaacg
    24421 ccagccgctg tggaaactgg ttctgctttt tgcgctatgg ggtaatctgc atggtggatt
    24481 catctggggc gccctgctct tgttcgccac ccttgcaggc gaatcgttcg atcttctatt
    24541 tggaaattct ccaaaacttg tattcaaaga gatcgggcat ctggctttgt ggagtctcgc
    24601 cgccttcctc gccgtggcat tcaacccgaa cggatttgag ctttggaaac tacccttcca
    24661 gcaagtggat gtttcattgg caattcaaga atggctttca ccagactttc accagatcta
    24721 tgcccaccca atgctttggc tgatgttttt gctggtcttc ggactcgggt tttcaggaaa
    24781 acggctttcc ttcaccgatc tattcaaagg attgggattt gcttatttgt tcttcttcgc
    24841 gcagcgcaat ttggtagcct acaccatcat catcctgccc atcgtggaaa agttccttgc
    24901 ccccgcatgg gcaaatctga ccgaagcgcc gcaggtgaag gcgtttttag aacgcttttc
    24961 cagacaggct tcgaaaggac agttttcgcc aggggttgca ggggtgatca ataatgcagt
    25021 gatcggaatg ttcaccatcc tgctcgtagg atatgcctgg tcggtttcaa tgcccgcaag
    25081 catcgcaaaa gaagttcctg cacaggctgt agcgtgggtc aaagaacacc aaccgagcgg
    25141 acccatcttc aattcatata actggggcgg ctatctcacc tggtctttgc gtgagtatcc
    25201 cgttttcatt gatggccgtg ccgacttgta tggaaacgac ctcattcagg aatgggtgga
    25261 gatttcaaac gggaccgaaa ctggtctgga gcggctcgaa tcgcggggaa tcaatttgat
    25321 cttcctcgaa ccctatcaca ccctgttgga aaaactcgac ccgcaaacat gggttcaggt
    25381 gtacagcgac agtcaaattg tcatttatca aagaaggcca tgaaacaagc tcaatctcac
    25441 gaaaaggatg tcactggatt tttcctgctg ctggcagttg tctttgggct ggcagtgcgg
    25501 ctgtacccca tcctcaaaac agacttcccg ctggtggatg gaggcatgtt ttacacaatg
    25561 gtcaatgacc tgcgggcgga agggttttcc ctgccactgg tcacatccta caatcaggcg
    25621 gatattccat ttgcgtatcc tccgcttggc ttttatctgg cagggctgat caatttattg
    25681 acaggcatct cggtgctaaa catccttcgc tggctgccat ttttgatttc gatgttgaat
    25741 attccgcttt tttatttcat ggcagaacga ttgatcgaat caaaaccgaa ggcagcattg
    25801 gctgcattga tctttgcgct cactcccagc gcctactggt ggaacatagt cgggggcggg
    25861 ttgacacgct cattgggcgt tttctttttt acagcggttg ctttttgcgt tcaccaaatg
    25921 tttcagcgca ggtcgtggtc ctgggttgtt gcggcaggtt tcagcgcagc aggatgcgtg
    25981 ttgagtcatc ccgcctgggc tgtgcaggcg gctgtcgctt cccttgtcct gtggtatttt
    26041 ttcggcagag atcggcaggg ggtgttttat gctgtaatag tcgccatcag cgttttgctg
    26101 ctgacatcgc catggtggtt caacgtgata cgacatcacg ggcttgaaac attgctccat
    26161 gcagggcagg gtagttattc gcggttgaag atctggacgg tctttttcac gctgtcgttc
    26221 actgatgaat atactcccgt gatcgcggtc tttgcactgt gcggaatttt catgcacgcc
    26281 gcaagaaaag attatctccc aattgcatgg gcggcaatgg gtttactggc cgaaccgcgc
    26341 ggcggcttgc ctgcatccac tttgcctttt tcgatcatgg cgatgacggt gctttcagat
    26401 ggcatcgcca cgaggttgac cccgtcaact gaatggatgg attccctcaa acaacacagc
    26461 ggacgatcgt tcttcggctt cttcattctg ctgttcacgt acaacgcctt tcaagtgtca
    26521 aacacactct cgtaccaggt cttgacgaag gaagaacgga cagcgatcga atgggtccgt
    26581 agcaacaccg accctgccga ccgttttttg gttttggatg atgaaggcaa tccgctgcac
    26641 tccccgctga cggaatggtt ccccgccctt tcagaacggc gcagcgttgc cacgattcaa
    26701 ggaaccgaat ggctgagtgg taaaagtagt tacgcaaatc aatacgacgt ggtcaaaaac
    26761 attcagcaat gcctgtacgc cgacatgaat tgcatccaca atcttgaaaa cctgctgaca
    26821 gatgaataca gttatatttt gatctccacc gctggagggc aaagcccgtt gggcagcgac
    26881 ttgaacacgc aggcaaacgc ggcactggta tattcctcaa cttctgtgaa agtgtatcgg
    26941 gtcaaatgaa aagtcaaaaa gcgaaactaa ttcttgtgct ggtgatattt ctgctcacac
    27001 gcttggcagg gcttgtcaca cgccccattt ggtacgacga agcgttctcg attttattct
    27061 ctgaaaaggg actgaacgca atgatctacg gtacgctgag tcagagcggt tctggcgcag
    27121 ctgatattca cccgttggga tattacgttt ttctatgggc atggatgcgg gtcttcggca
    27181 cgtccatcct tgcggcacgt ttgctatcca ttttcatcag tcttgccgcg ctggtcttgg
    27241 tctacttgat cgcacgcgaa ttattcgatg aaacgaccgc ctggatttcc gcactgctct
    27301 tctccatcct tccgtttcag gtccacttcg ctcaggaaat ccgcatgtat atgctgctct
    27361 cgttctggct gctggccgcc acctacgcct ttctgcgcgg acgcagcggc gagtggaaat
    27421 ggtggctgct ctttggggtt tgcagtgtgc tggcacaata cacacatcat cttgcaagtg
    27481 tgtacttgat tccacttgcc cttacgccgc tgtggcaaaa ggactggaaa acgctcaaat
    27541 cggtcatggc aggcgcattg ctggctgtcc tgctttacat tccgtggctc attcatctgc
    27601 ccgctcagct ttccaaagta caatcttctt attgggtgga gcgccccggg ctggaaaaaa
    27661 tatttacact gattctgttc taccttccgc atctacccct gcccaacgga ctgcttttgc
    27721 cagggctgct gatcgccatg ctggtgatcg ttctggctgt tttccaaacc atccttgcaa
    27781 aacgaaacct catcgccaat acaggcaagg ccacatggct ggcaacgctg gcatttctcc
    27841 cgccgctgct tttgtgggtc atctctcaat ttgtccctgt ttatatcgaa agagcccttc
    27901 tgccttccca tgccattttt tgtatctggc tggcatgggc gttcactcaa acaaggctgc
    27961 ctaaccctgt gcgaattttt acgttcacgc ttatcggtat cgctgccgtc atcgggctct
    28021 cacagcattt cttctatcag ggttttccct acggcccttt ccgtgaaatc aaccaaagca
    28081 ttcaaagcag attggaaaac ggcgatgcga tcattcactc cagcaaactt tcctatctgc
    28141 cctcgttcta ctttaaccgc aagctggttc aaaccttcat cattgaccca ccccaaagca
    28201 atgtagacac acttgcccca gccacgcgtg aggtactgca gatccgctct gccgaagaca
    28261 ttgaaacagc ctccgcaaat gccacgcgga tctggttcat catctaccaa caatctgtgg
    28321 acgaatacac agcacaaggg tacaaaaccc atccacacct tgaaggcctc gaagaaaatt
    28381 acgaactcga atttattgaa aactgggacg atgtcaggat ctatctctac gtgaaaaagc
    28441 cgtaaaaaaa tcctccgagg ttctcggagg attttcactt ctacaccacg gcatcgatca
    28501 tcaacgccag aaaaataaac gccagataca tgctcgacca gcggtacatg gtccatgcca
    28561 ctttgttgcc gcccactttg aaaacacgcc aggcagcata taaaaggaaa cctcccagca
    28621 cgatcgcaga tacggcatac accatcccag ccaggttgaa gatcggaagc aacaacgtga
    28681 ccgcgatcaa ctcaatggtg taaaccagaa tatgcctgcg ggtcttttct tccccatgca
    28741 ctacgggcat catggggacg cccgcctttt cataatcttt catacgcaca atcgccagcg
    28801 cccaaaaatg cggaggggtc cacatgaaga ctatggcaaa aaggatccag gctgcgagtc
    28861 ccaattgacc agtcgccgcc gcccacccta ccatcggcgg aatcgcccct gcgccaccgc
    28921 cgatcacaat attttgcaca gttgccttct tcaaccagac actgtaaaag aacacataat
    28981 agaaaatacc agccagagag agcagggccg ccaataaatt cacgaaaccc gccatcacat
    29041 aataactggt cagacacagg gcaaggccgt aggaaagtcc ctccgctgcg gtcaggcgcc
    29101 catctgcaag cgggcgcttg gacgtgcgct gcatattctt atccagttcg cggtcaatgt
    29161 attgattcaa cgcgctggag cccgccgccg ccaacgtacc gcccaacagg gtccagaatg
    29221 tcagcgaagc cgaaggccat gcctttgagc cagccaccaa tccgctgtaa gtggtcacca
    29281 gcaacaacaa aacaataatg ggcttggaaa gaatgaaaaa atccttcgcg cgttgacggt
    29341 aatcaaactt caccacttcg tttgaatcca ccgccagggc tcccgaaaga taaaccagcg
    29401 aacccaaact gatccacagg gcaacagcgg tcaaagcatg caaaaccacc agatgtgtgg
    29461 ggtaggaacg ggtcacttcg attgccccga tgaaagcctg cccgaagaac aacacgcctg
    29521 tcacagtggt caacggcaaa atcacctgct gatggcgctg ttcgcgccac gccttgcgca
    29581 gcagccaaaa catcaacacc atggccaatc ccaccagaat cagatgggcc agcttcaaat
    29641 acccaatcgg ctctgaaggc acacacaaag gccagcccaa acaatatgcc catgcacccg
    29701 tcaccgtcac gatccgccct acgatggtca gcaaaaagac catgatgaac agggacaatg
    29761 tcaaacgcgc aaaagatgaa cgaagagaat acttcatacc tgctattcct gctttcgcaa
    29821 ataaaatgga tacccgacaa atagaatggt cgcaaaagtg aaccactgca aagcataacc
    29881 gaagtgcgaa ccttcggtca actcaataat tggctgatat ggaatgggcg gaacctcatc
    29941 ggcaggctcc acattcggct gaatataaac cccaagaatg ggataaggaa actgcgccga
    30001 catattctcc acgtcgagat tattccacac ctcaagcctg gtccccgccg caggcagcgc
    30061 atctgcgatt ccgccgatgg ctggtttacc atgcccaagc cgaatctgcc ctgttacagt
    30121 cagagtgccc tgctcatcat attggcgcca atcggcgggt gtggcatttc catcagcggg
    30181 gatccagccg cgatccacca gaacagccgt cccgttggaa agaagcggcg taatgaggtg
    30241 gtatccatat tgatcgccgt tgtattgatt ccgtatggcg acctgattct cgaaatcata
    30301 ttcacccgtc accgtgaccg cacgccattc catcgcagcg atatcctgcg gaattgcctg
    30361 attcaaatcc agcatttccg ccgcacgcat ggattcgacc tgcgcattaa aagcacggcg
    30421 ctgttcaagg cggtccaatt gccaaaaccc aagacgaatg caaaacgccg tacccagcaa
    30481 aacgagcagg gacgcaaaaa accatttacg cgagattgcc ttgcgaagaa ccatcatctt
    30541 ttttctcgtt atcctttttc ttcttctctc cgatcaatcc gccatatacg gcaaacatca
    30601 tgccaagcgg aaacccagca aagaaaatcc ctgaaacacg cgtgcgccag gtaactggct
    30661 ccatcactgt caccccgaag ttattgtcgg cttgaaatgt gcattcaaag atcaaattct
    30721 cggcggtttc aagtttgagg ctggccgaag actcggcagg gatccacaac gggcccagtt
    30781 gatgattctc atcatcctgg ttgatcacgc gcaaagtatc gcctacaaca aaacgcatat
    30841 ccctgggaat ttccactggc gcattcccag cgcggatcga atctgcagta ccagcgggga
    30901 tcaccaattc aatgagtcct ggtgcgcggt tttccttttt caaaaaaaag taagccgcct
    30961 cggtggtaat ggcagccaat acaagcccga ggagaaatga aattactatt cgtttgatgg
    31021 agaaattcat ggttcccgct ccaatagcag cttgatatcg tggacaatat catcaacagg
    31081 ggtttggtag gcataggaaa gacgcaaatt gccctgtgaa tccacaagga aaaggcgggc
    31141 agtgtgatta attatgacgc caaaagccga gtcgctttgc acggcttcgc ggaagatcga
    31201 gtatgcctgc cagacgggct caagctgctc tgaggaaccg ctcaaaccaa tgaacgctgg
    31261 gttaaagtgc tccacgtatg tttgaatttt ttcaggggtg tcccgttcag ggtcaacaga
    31321 aacgaacaca acctgcacac gcgaaccatc atcccccaat tgatccatca ccatcttcat
    31381 ttctgcaagc gtggtcgggc atacatccgg gcaggaggta tacccgaaaa aaagtagaat
    31441 gaccttccca tgctgttcgg tcaggcggaa tgtttgtcca ttcgccatag gcaattcgat
    31501 ctctgaagcc cgcgggtacg gttcgccata caaagcaccg cgaaaactcg ctggtttaag
    31561 aaaaaaaata ttcaaaacaa aggccagtgc cacgattccg agtgaggcca ggcctacgaa
    31621 ggtgatgcgc ttatccatga tatgttcttg ctccaagtgt aacgaacagg tacgcttata
    31681 tcaaagtttg aagaattttt ccaataaaaa accgccgcct aactcggcgg cggtcctttt
    31741 ttagtttact aacttgccga tcaaatatag cgcagggtag aagaagatcc acaccaggtc
    31801 tacgaagtgc caataaacgg cggcagcctc cacgccccag tgcttttcag ccgagtagac
    31861 gtccttgcgg gcattgttcc acacgatcag caggaagatc aacccagtca acacatggaa
    31921 ggcatgcata cctgtcatga tatagaacac gccgcccatc gggccaacgg cagggttgcc
    31981 aaagctggcg gtcaatcctt cttcagcagc gaggcgccat tccaccatca ctacaccgag
    32041 aaggaagcct agtcccagca cgaaggtgat gagcgtattg ttcatgaaac ccttcttgtc
    32101 accatgagcc atggcagttt cgccgcggtt catgaagaag gaagagacca gcaggaccgc
    32161 cgtcaccgcc agaccgaggg tctggttcaa gtgtggacgt gtcaacccga gcaggttgat
    32221 gcgggtgacc aacaggcctg cgaatacgaa accatccgag agcaggaaca gccataaacc
    32281 gatgcggttc gtccccgttt tgtaatcgta ggagtgttca tcgccttcgc tgtcttcatt
    32341 gagcttgtaa atgtgcatgt agtagtacat gacaagagca gccttaatga cagccaccac
    32401 cacaaggatg gataccgcat tgaaggtaat agcgacgaaa tattccagag cggtcaaaat
    32461 ggcaaggtag atgaaaatca ccacgccctg accgtaagcg gcagatttgg tttgtgtatg
    32521 tgacattgcg ctcctcccct ttacttgtgc gcgccagatt caggcgcgga gaatttgatg
    32581 taggcatgtc cgccttccaa accgtaatca taaggttcgc tgattacttc gaaaggctgg
    32641 tcataattgt gtacaggcat gggagaagga acctgccact cgggtgagcg ggaattccag
    32701 acattgccct cggctttttc gccgttcttg atgctgctga agaagttgta gatgaaaatc
    32761 accacagaaa gggcaatgag gtaacctgcg atcgtgagaa caaggtgtgt tgcatcaaga
    32821 ccaagcgccg ggtcgtagtc cacgatgcgg cggcgcatcc ccaacagacc cacatacatc
    32881 atgccaaagg tcaaaacgaa gaacgagggg gtcatcagcc agaaatgtac cttgccgagg
    32941 gtttcgttca tgcggcgacc ggtggccttc gggaaccagt aatacaaggc ggcaaagaat
    33001 gggaagacaa agccgccgaa aatggtatcg tggaagtggc caacgatgaa gtaagtgtca
    33061 tgcaggtgca ggtcggtgga aacgatcgca tttggcgggc cagaaagacc acccataagg
    33121 aatacgacga taccgcccaa aacaaacagc atgggagtgg gggtcgaaat cttgccgccc
    33181 caaagggtgg ccacccacga aaagaacttg acgcccgtag ggatggcaac aagaagggtg
    33241 ctgtacatga acggaacacg caggtattcg ttcatgcctg aagtgaacat gtgatgtgcc
    33301 caaaccacaa acccaaccaa cgcaataccg agggatgaca tggcgatcca gcgataaccg
    33361 aacaacggct tgcggacgaa tacaggcaac aactcagaga tcacaccaag gccgggcaga
    33421 atgaacacat acacagcggg gtgcgagtag aaccagaaca ggtgctggaa gagaacagga
    33481 ttaccacctt tggcggggtc gaaaaagccc atgccgaaaa ggcgctggaa catgaccaac
    33541 tggaaggaca gaccgatgaa ctgggtggca gtcaacgcaa tgagtgaggt ggcaattgca
    33601 gcccaaacga aaataggcat ccgcattgcg gacataccct tggcgcgcat tcgcaatacg
    33661 gtcacgatca cattcaatgc gcccaaaatg gacgaccaac ctgccacgaa cactcccagg
    33721 aagaacatct gcatgccaac aggggcacgg gcagaaagcg gcgggtaacc agtccaacct
    33781 gtatcaaaac cgccgaggaa gagactcatc aacagcaaaa cggcagcagg aacagcgatc
    33841 cagaacgaga acgcattcaa acgcggaaaa gccatgtctt gagcgccaag caacagcggc
    33901 acaacatagt tgatgatacc ggcaatgccc aacaaaatgg acacgatcat gatcatgcca
    33961 tgcaaagaca tcaacgtgtt ataaagctgc ggaccattct gaccaaacaa ttgcatatct
    34021 gtggtcagga attgaagttc agatgccgca agttctgtgc ggaagatcag ggcgaacaaa
    34081 ccaccaatgc tgatcaacgc caaagccaca atggtatatt ggattccgat gaccttatgg
    34141 tcaagagaaa cattgacgaa cttctcccag ccagagtgat ggtcttcgtg atgttcaggg
    34201 gtatcaatac cccgtgccca cttgacccaa tcgctggtaa ccccggaact gaacagaaag
    34261 gcaatggcgc cgataaaacc acccagcacc caggcaggtt cagtgaagaa aaaacggtcg
    34321 gtaacgctca accccatgag cgcacgaata ccagtgacca gcccaccgcc caccacggtg
    34381 ccgatcaact gccccaaaag gccatgtgcc aaagcacttg aaatgaaacg cttcatataa
    34441 gaaatcctcc gccagattat ttcagcgtct taagatacgc gatgatgttt gcaatctctt
    34501 cctcggtcaa tgcaaacgga ggcatgacag acggggacgg gaaaccttcc acaatggtcg
    34561 cgttcgggtc aagaatggac tcggagataa aggcttcatc agccgtcaca gtggaaccat
    34621 ccgccagttt cacatccgcc ccgaacaagc caaaccatgt cgggcctgtc atcgcagtgc
    34681 cgtcaatgga gtgacaaccg acacaaccct tcgaaactgt caacaattga cccttgccct
    34741 cgggggtttg agctgcggca gcaatcgcaa tttgctcggc gatccaggct tcgtaattgg
    34801 cttcctcgct cacaacaatc ataccttcca tgtaagcgtg agaggtaccg cataactccg
    34861 cacaacgaac tttatagaca ccgatcaacg tcggtgtgat tctgtactcg gtcacacgac
    34921 caggcacaac atcttgcttg acacggaatt caggaaccca aaacgaatga atcacatccg
    34981 cagattccat cttaagcaac acctgtttat tcgcaggcaa atgcaactcg ttgctcacca
    35041 ccccatattc cggatattcg aatgtccacg caaattgctg cgctttaacc ttgatgacaa
    35101 ccgcctcagg atctacgcgg cggacttcac caagcgaata cgcccccata tacgcaaaaa
    35161 taaccacaag gatcaacgga ccaacggtcc agccgatctc caaagctgtg ttaccttcaa
    35221 cgtgctgggc gtcggtatca tcgcccttgc gctttcgaaa cacaaccagg ctgtacgcca
    35281 tcggcgcaac gatcaaggca aacaagaaag acatggcaac gatctcccag tgccacatat
    35341 tatcgattgt ctgcgcctga gcgcttgctg caacagggtt catctgtgtt gcaaaaccgg
    35401 cagcgtcaag acctacataa gtgagaacgg ccatggcaat gaccaaaatt cccacgatca
    35461 caaagtgtcg catatgctct ctcctcaaaa aaattcaaac ggggatgccc gccccaaact
    35521 cacctaattt accaaatttt ggtcaaattg tcactatttt ttgtgggcat tgtacgcatc
    35581 ttccctaatc tgattcatgg ctccgtccga aaaacaggat cagcagagaa gcaaaaatgc
    35641 gaccaacctc agctaacacc aaattccttc aacagttcag cactatgttc cgcagggcgg
    35701 acattctcat acactttttt gatcatcccc tcgccatcaa tcaaaaaggt tgtacgcaaa
    35761 acgccctcat aggccttccc catgaacttc tttggccccc acacaccata ggcatcgcaa
    35821 accttgtgac catcatcagc cagcagcgga aattgaagct gaaatttctt tttgaatttc
    35881 acatgcgaag ccacatcatc agggctgacc cccaaaatga ccacccccgc cttttcataa
    35941 gcagaataat catcgcggaa attacaagct tccttggtgc agcctggtgt atcatccttg
    36001 gggtaaaaat ataaaacaac attcttgccg cgaaactcag acagctttcg caaggcattt
    36061 gtatcatcaa gcatttcaaa ctcgggtgcg ggaattcctg atgggatggg catggtttaa
    36121 tctccttttt tataagggtc gtattataca catgcaaaca aaaaacggca cgctctgcat
    36181 agagcatgcc gttttttgtt taataccctg atctacttac ttgagcgtgc ggatgaacgc
    36241 aatcgcctgc cagatctgct cgtcggtcaa aacacccttc caggcaacca tggatgtacc
    36301 ttctttaccg gtattaatac gccaatacag ataatcatca ccagcctggg cggcaagttc
    36361 gggcaaattc ttcggagcag ggtcaagtgc cacaccggca gggccatcac cgtggccttg
    36421 cgggccgtgg caggattcgc agttggtctt gaatacttca gcgccagccg cagcggcgtc
    36481 agcggcaaga gggttggcct gacctgcata ttcggcagga acaggagcca aagtagctac
    36541 cgcattcgag cccccaccac aagctgccag agcgagcgca gccaaaacta aaacagcaaa
    36601 caaaactttc ttcattacta cctccgtatg tatatttggt tcgacagcgg ggcgtattat
    36661 atacattttg tgaatttaag tacaggattt taatccccct ctcacacacc cttttttcgc
    36721 catttcaaca cgtagacaga aaagtggggt tataacccca aaccacttat aacaaggaga
    36781 aaatcatgaa tgcattgact ttagtacttc ggcttattca tatcgtggca ggtgtatttt
    36841 gggtcggagc cgcctggatg ctgacactgt atattgtgcc cagcgcaacg gctgttggcg
    36901 acgccggaca acaattttta cggcagatga tgggaaaaac caattttcag aaaatcatga
    36961 cgacttcctc cctctcaacc gtcattgcag gaactatcct ctatgtccgc aatccaccca
    37021 catggttcag ctcgaacgcc ggcattgggt tcggcatcgg agcatttttt gcgttgatcg
    37081 gcctggtatt tggtttctta attggcaaaa ccaacatggc catgatgcaa atcggctcac
    37141 agatcaaagg ccagcccacc ccagaccaga tcgcacaaat tcaagacctg caaaagcgac
    37201 aacgcacttt ctctaccatc aacatctatg cattattagc cgcagtggtt ttcatggcaa
    37261 tctcccgcta tctattcatt taataaacca aagcaaacac ctgcctgaag aaacccgggc
    37321 aggtgttttt ttatttcgca tttcaattct tatttatgac cttggcaact tatggaccaa
    37381 ttccctcaaa cttagtcaag gtcatcggct tttttcttcc ctttcggcgc ttcacgcgga
    37441 ccttcagcct gcccctgttt ctttgagcct tcaggccacc cgaagacaat aatcatgacg
    37501 aagaacgcaa ttccaatgta caacagccat gacagggaag tgtctggccc atgcccaccc
    37561 tcagataaca actgcaacgc aagcatatct gcctccagaa atttattttc agccatccga
    37621 aaaacaccat ctacggacag gatttgatac ttataagtga aaaggctgtg catgtcaatt
    37681 ctgagggtaa aatagcacac acttacatcg gagccgtaca gatgaaactc aaaacttggc
    37741 aaaagattct gggcattcta ttgattgcca ccatcctcct ccgcgtcaca tccagacccg
    37801 cagtggatca tccctactac gccgcagacc tgaattaccc cctcgtcatc gcccaccaag
    37861 gcggagatgg cctctggccc ggtgacacca tgtttgcctt tcaaaacgct gttgacctcg
    37921 gagcagacgt gctcgaaatg gacgtccaca tcacaaagga tggcaccctc gtcctgatgc
    37981 atgacgaaac tgtggacaga accacaaatg gcacaggcga gatcgaatcc atgacgctgg
    38041 aagagctcaa gcagtttgac gcaggttatg actggacgcc cgatgaaggc gccacattcc
    38101 cctttcgcgg acagggtgtc acaatcccca ccatggaaga ggtctttgct gctttcccag
    38161 acaagcacat gaccatcgag atcaaaaaga caaatgcttc aatggcagga ccattttgcg
    38221 aattgatccg taaatacaac atggaagaca aggtcttgat cgcatccttc catgacgaca
    38281 gactcaagga attccgcaag gaatgccccg aagtggcaac ctccagcgca aaaaatgaaa
    38341 cgacggtttt tgttctgctc accaaaccgt ttcttggcag tttttactca cccaagttct
    38401 tctccctgca agtgcccgaa gaatctggcg gcatcaccgt aatgacccca gcctttgtaa
    38461 aagccgccca cgagcggaac ctcgctgttg agccgtggac catcaacgat gaagaaacca
    38521 tgaaaaaact aatcgcctgg ggcgtggatg gaatcatcac cgacaggcca gatattatga
    38581 taaaggtatt gggaaggtaa aacgcaatga catcctacga agacagcatc gagaaccacc
    38641 gcatccaacg agacgaaaaa acagcagcga accagcttca ctggttaaat ctggcaggcc
    38701 tgttctggct ggaaaaaggc gaaaactctt tcggaaacgg cacagacaac aagatctgcc
    38761 tgcccgcctt tccaagtgcc cattgcggct ccttccacct cgaaaacgga atcgtcaccc
    38821 tgcaccctgc caaaggcgct gatatcactg taaacggaaa gaccccaaac gcaagtccat
    38881 tgcgcactga ccgtgaaaaa gaccccgatt tgatcagcat cggctcactg accctgaaaa
    38941 tcatcatccg cggcggagag ccattggtgc ggatctggga tcgggaatca cctgcgctca
    39001 aacaattcga tggatttcat tattacccgc ccaaacccga ataccgcgtc atcgcaaaat
    39061 acgttcgcta tgacccaccc aagccgatca aaattacgga agtcattggc acagaagttg
    39121 agagcgcatt tttaggtcag gcacgtttca cattaaatgg cgttgaatgt gtgctcgaag
    39181 ccgaaaaaag cgacgacgaa ttgcttttca atttcaccga tggcacgaat aaagatacca
    39241 cctatggcgg cgggcgaaaa ttttacctgc ccatccccaa gggcgatgaa atcgtcctcg
    39301 atttcaacct cactgaaaac tggccgtgtg cctacacgcc ttatgccacc tgtcccattc
    39361 cacccagaga aaaccgcctg ccgatcaaag tggaggcagg cgaattaaaa tatcacgatt
    39421 aagcaaaaag agagtaacga ggctggcctc attactctct ttttttcatc tcttttcttt
    39481 cttgatcact ccatccaaac agttcgatcc tcaaacccaa tacgggtgta cggttctggc
    39541 gtcacttctg gataccccac ataaataaag ccgacgatat gttggtctgt ggaaaaaccc
    39601 agaaactcct tcaccctggc atcccgtgcc cattcacctg tgcgccagat tgccccaagc
    39661 cccagtgcat gagccgcaag caaaatattc tgacaggccg cagaagccgc cgatagattt
    39721 tcaacttcaa tggtctttga atcagcggcg ggtttatccg caccgacagc aatcaacagc
    39781 ggggcgcgga gcgacaatga tcggggtttg ttcaaacctt cgggtggcgt ggcaggatta
    39841 cggtccagaa aagaagcggc cattacatcg cctaattttt tacgcccgtc cccagtcaac
    39901 acaacaaagc gccacggtct taccttatga tgattgggag cctgcgctcc cgcacttaac
    39961 aacttctcga tcacatcacg cggcaatggt tctggtttca ccttgccgat cgtcaatcgt
    40021 ccatgaattg cttcaaatag ttccatcgct tcctccgccg tcactgacgc ttgcgggtaa
    40081 aattgtctta ccatgaaaat attttattct gaagaacacc gcagccatta tccgccattt
    40141 gaagtttttg acggaggcaa acgcattccc tactacgaaa accctgatcg tatggataga
    40201 attttggctg cacttggaaa aacggattgg gcggaattga ccgagccaca ggatttcgga
    40261 cttgacccca ttcttgctgt gcatgacggg aactatattc aattcctcgc ctctgcctgg
    40321 accgagtggc tggcctctga ccctcaagtt gcagcctcgc ccgagcagaa cgcattcctg
    40381 ccagccacct ttgcattgcg tcgcaagccg cgtgtgccga attcactttt gggcaaagct
    40441 ggctactaca tcatggattt atccgcctgc atcgtggaag cgacctacaa agccgctctc
    40501 gcttctgcaa attgtgcttt aagtgcggca tcttcctccc tcatccttca gaatgcatcc
    40561 ttcgcccttt gccgtccgcc tggtcatcac gctggcaaag attatgcagc aggatattgt
    40621 ttcatcaaca acgcggctgt ggcggcaaac tggctttctc aaaaaggaaa agtcgccctg
    40681 cttgatattg actatcatgc aggcaacggc actcaggata tcttttacga acgcgctgac
    40741 gtgttgacca tctcacttca cggtgaccca gattttgaat acccaagtta tattggcttc
    40801 gctgatgaaa caggcgctgg cacagggctt ggctttcaca aaaactttcc gctccccaaa
    40861 ggcaccgatg atgtccgcta cctcgccgcc ctggatgagg ccctgacaat gattcgaaac
    40921 ttcgcgccgc aacaccttgt cctttccaca ggcatggata catttgacgg tgatccgctt
    40981 ggcacattcc atgtcacacg tgacggtttc agtgaaattg gaaaacggat cgctgcgctg
    41041 aaccttccca ccgccatcat catggaaggc ggctacgcaa atgaagcgct tggaactaac
    41101 acagtcacct tattggagaa gttcaaatga gaaaaggtct tatcaactac ctgatctgtg
    41161 ctgtcatcgt ttttggttgc agcctgcctt cacaagcgcc cacagcaacc ccgctcccac
    41221 caactgctac agaaacaccc gcctttacca acacacccga attcaccgcc accccgcttg
    41281 ccacactaac atccacactc accctcacac cgaccattac attcactccc accatcaccg
    41341 ccacaccgac cttttcgttt cccagtgtca cggtcaataa acaggcacac tgtcgttatg
    41401 gtccttccgt ggcattttta catgccgccg acttgtaccc cggtgacaca gggtacgttg
    41461 atgggagata cgaatacagc tcatggctac acgtaaaatt cgacaaattg aaatattatt
    41521 gttgggtcgc cccatcggtt gtggatgtgg ttggcgatat cagcaccctg tactataacg
    41581 agccagatct gcaatcagtc ggttacagca actatccccc gccgcaaaac atcgttgcca
    41641 tccgcaacgg tgacgaagtc accatcagct ggaatcaagt agaaatgacc accgaccacg
    41701 accgtggtta tctcattatt gcttttgtct gtcaaaacgg cgcctattta tggtggacct
    41761 tttcctaccc tgatcaattt acaaccagct acaccgtaca ggatgaagca ggctgtgcgc
    41821 aaccctcgga aggcgtttta tacacggttg aaaaacatgg cttctccaag ccagcacaaa
    41881 ttccctggcc tgcgccttag caggagaatg cctttgcaac ggcagcaacg atgacggcaa
    41941 ggatacagaa gaacaaaaaa tatatgatcc cgccaaccac cagtggcgat gtttttcctt
    42001 tgctcaatgt aatcccaagc caggctgccg ccccagtgat tgcgcctgcc agcaaaccaa
    42061 ccacagggaa aatgaaaact tcgataaaaa actgcgaatc ggcaaaacag tcaaacccaa
    42121 aatagccgct tatcaaagct actgccactc cgcccaccac accaacaccg cctgacaaaa
    42181 agcccgtcaa gcaccccgtc ttccaaccgc ccgttttttc atctggagcc atcgtggttt
    42241 tttcctgttg gttcatcggc agaatcctga attaattttt taaaaagaaa aagccatgca
    42301 ggaatccata ttcccagaca tggctttttt cgtttcgacc catattacgc aaaatatttg
    42361 attatgaaag aacacccgaa cgtttaaagg cattctccag agcgtcaagt gtagcgggtt
    42421 taatgacaac cgcctgggcc ccgccgcgca ttactctttg acgagtctca ggctggtcat
    42481 ctgatgtgat gacaattacg ggaatcttca tcaagcgggg ttcgcggcgg agaaacgcca
    42541 gaacctccgt gccatccaca ccaggcatat tgatatcaag gcagataaaa cctggcacaa
    42601 aactgctcaa catcgaaatt gccgggcttg aaccatatgc aacacgggcg ggtagatcca
    42661 acacccctaa catttgttga agggcatctg ctgtctgtct gttgtcgtcg atgatcaatc
    42721 cgtcttccat gaaattagtc ctccatgatt attctaccca ttacatttac atcatagcct
    42781 gttaatttcg atacagaatt tcgaaaaaca ggatcagtca agaggtcgaa gagaggcgcg
    42841 agcaattcac tttccgcaaa aaccttcggc attaccagat catagcgctc ttgaaagagc
    42901 ggcacaaaat ccaactccaa cgcctgggca gcggcggcaa ttcccaaccc gcaatcggca
    42961 cgccctgaag ccaccgcagc cgcaaccccc aaatgagtat attcttcctg agtataacca
    43021 gagatggatt cggaggacat ccccattaag ttcaagtgat aatccaacag gacgcgtgtg
    43081 cctgccccac gctggcgatt cacaaactgg acctcgtgac tggtcaggtc tcccaggctt
    43141 ttgattccct tcgggtttcc cttcttaacg atcaacccct ggtcacgtcc caccaatgaa
    43201 acaaccttca ccggaatatt cggcatatat tggcggatgt aggcaatgtt atacgaaccg
    43261 tcgcttggat ccagcaaatg agaccctgcc atgtgtgcct cgccgcgacg aagcgcaacc
    43321 aagccaccct gtgacccgac gttcgctgaa gccagccgcc gatcatgttc ggctaaaaac
    43381 tgggccagca gatccagggt catatcgtgc gagccgatgc agaagatcgt tttctcgatc
    43441 tgtgctttgc tgcgatacaa atgtactttg acgggttccc ccgcttccat gccttgcacg
    43501 ccactgggga tcaacgccaa tccatcagcc tgcaccagtg aggtgatgac ccctgctcca
    43561 cgtgaaagcg gcgccgcaag caatttatct cccacctgcc ccaccgccac gcggacaaag
    43621 tcatcgtcgc ccgcaggtga aacgagtttc cgtgtgagag tggcagtttc agtgggtaat
    43681 tccattggag ggcgtcccaa ccatttggcg atcaacgcct caacaaagat atccacagtc
    43741 agagccgcag agacagggta cccaggaaca ccaatgatgg gaatcaggtt caaggtttca
    43801 ggttttaggt tttctgattg acgattaacc attcctaaaa ttacagggtg ccccgggcga
    43861 accgccacgc catgaacgag gagtgtgcca agcttttcaa caactttgga tgagaaatct
    43921 tccgcgcctg ctgaagaacc agcattgagc agcacaaggt catgagactg tgaggcttca
    43981 aatacgcgtt cgcagatcag gccaaaatca tccctggtga tcgggtagcg ggtcggttca
    44041 ccgcccattt gtttgatctg cgccgccagc acaagggagt tgtattcgag gatatctccc
    44101 gattttagtt tggacccgat cgggacaagt tcagtgcctg ttggcaaaat ggcaactttc
    44161 ggcttaagtg cgacgcggat cgtctgatgt ccagaggcag cgatcgcacc gaggtcggca
    44221 ggtcgaagcg catgcccagc aggtaaaacc agttgcgtgg caacgatatc ctcacccaat
    44281 gggcgcacat gactccacgg cgcaacggca gaacggatgc gaatggatgc gggtttgcga
    44341 atctctttgg tgatctggtc gttctcgtca agcgattcaa cattttcgat cggaatgaca
    44401 gcattcgtcc actcagggag cgggtcgcct gtatccacat attgtgcttc aggccccacg
    44461 tggagcgtaa caggcgacga aggcattgcg ccattggtag agatcgcacg gacggcaaaa
    44521 ccatccatcg cagaggcatg ataatgcggc gaagaaattt cggcccaaat tggctcggca
    44581 gtaacacgtc ccagcgcgtt ttcgttgagc ggaatttcct caacgccaag tacgcgccac
    44641 aaatttgcct cttgcagagc ttctcttatt cgagcccggg cctgggcaag gggaatatca
    44701 tgaagataga cagacatggc aaatcgctgg tttaagattg aaggttgcag gtcgaaagaa
    44761 aatcttcaac ctgcaacctg taatttacaa ccttctatgc agaagcatcc attgcggcgc
    44821 caaggcgctt gatgatctca tccacttcat cttcgttgat ggaaagcgga ggtgcaaatg
    44881 caagcgtgct cccaagcgga cgagtgcgca gcccgcggtc ttgagccgcc ttgaagatct
    44941 gtgcggtctt tgcggcatcg cctgtgcggg cctctttgtc tgaaacgatt tccacaccac
    45001 agaccagccc caatccacgc acatcaccga cgaaggaaaa ttccttcagg ctttgcaagc
    45061 catcatacaa acgcttgccc aattcgcggg cgcgtttggg aaattcctcg cgttccatga
    45121 tttcgatgtt cttcaaacca accgcgcagg ccatagcgtg ccccgaataa gtgtagccgt
    45181 gcatgtaggc ttcggtatca gcggtatttt ccatggtctc gcggatctca tctgaaattt
    45241 gaatgccacc cagttgggca tatccgctgg tgacggcttt ggcaaaggaa agaatatcag
    45301 gcttcacatt ccaatgtttg agcgcaaacc attcgccagt gcggccgaac ccggtgataa
    45361 cctcatccgc gatgaaaagc acaccgtatt tatcacagat ctgacggaca agcgggaaat
    45421 actcggcagg cggaacgatc acaccgccca cacccatcac aggttcagcg atgaatgcag
    45481 cgactgtatc ttcgccttca cgcaggatcg cctcttcgag ttctctcgcc gccgcctgac
    45541 cgacggtctc acccgccttc aactgcccct cgtaccgata cggattcggc gccggaatat
    45601 ggacaaaccc atccatcgcc gggccgaaca tggtgtgata tttctccagg ccggtggcaa
    45661 aggtggccgc cagtgtaata ccatgatagg aacccttgcg agcaatcacc ttgtatttac
    45721 cgggtttgcc tttgcgcttc cagtaatagc gcgcggtctt gaatgcagaa tcattcgcct
    45781 ctgatccgcc ggaagtgaaa aaggttgtat tcaaaccctc ataagcaaac cctgccagtt
    45841 tatcggccaa tgtaatggaa ggcaggttgg tcatacccga gaagttggac gtgaaagaca
    45901 tcaccttcat ctgttcgtag gcggctttcg ccaattcctc acggccatac cctgcgctga
    45961 cattccacag ccccgccatg ccatcaagga ttttctgccc atcggtagtg tacagccaga
    46021 caccgtcacc gcgttcgatc accaacggat tggcatgtga tttggggtga taggtcgggt
    46081 gtaactgtcg cttgtctttc tcgatccaat tctgtgtttc ctggtttgtc atattggcct
    46141 ccaaagaaaa atattaaaca caaaggacac aaaggcctca aagtttaaaa gcgtaatcga
    46201 ctttttcctt attcatcctt catccttcat aattcatcat taaccaagca cttccacatc
    46261 atgcggacca gattccttga gccccgcccc ggtcatccgc acaaaaattg ccttcttttg
    46321 aaattcctca aaattatgcg caccgctgta actcatgcct gattgtaaac caccaaccag
    46381 ttgtgtgagc acttctcttg ccctgccgcg atacggcacg gcggcctcca caccttcggc
    46441 aacatactct tcaatttctt cttgtgtgag gtcgttgcct tccttgcgat tgcggtcaat
    46501 attggcttca cgtgaagcca tgccgcgtga agctttgtag cgatgaccac ggcgggtcat
    46561 caccatgccg gggctttcat cggtgcccgc aagcatcgag ccgatcatca ccgtctgggc
    46621 acctgcggcg atggctttgg ctacatcacc gggctggcgg atgccgccat ctgcaatgat
    46681 ggggattccg gcagagcggg cagcctcggc acattcgatc actgctgtca attgcggaac
    46741 acccgaacca gccaccatgc gtgtgatgca gatcgagccg gggcctacgc ctacctttac
    46801 cgcatccacg cctgcatcga tcaagcgctt cgtgccgtcg gccgtggcga catttccgcc
    46861 gataatttgc gccgcaggaa aatgcttgcg gatatttttg atcatatcaa tttcgaggtt
    46921 tgaatcgcca tgagcgatat ccaccacaat gcaatctgcg cctgctttca gcgcggattc
    46981 tacgcggcgc atttccttgt cacgcacgcc caccgccgca ccgacgatca accgtccgcg
    47041 ggaatctttg gtggctttcg ggtactgtgt gatcttcatg atgtctttca aggtcaccag
    47101 ccctgccaca cgcccatcgg cattgaccaa cggcagcttt tcgatgcggt gttcatgcag
    47161 caggccttcc gcttctttta gcgaaatatc gggcgaggca gtgatgacct tcttctgcat
    47221 caaatcagtg acggattttt tgtcatcgtt ttcgaaaagc agatcacggg tggtgacaat
    47281 gccgacgagt ttttcttccg catccagaat caaaatgccg cctgcgcctg tttcgtccac
    47341 cagttttttg atgtcgccga cggtgtgggc ggtgctcatt gagatcgggt tatctacaat
    47401 gaatgactcc gccttcttta cgcgctggat ctgccgcgcc tgctcgtcaa tggaaagaaa
    47461 acgatggatg atgccaatgc cgccttcacg cgccatggcg atcgccattt cactttccgt
    47521 caccacatcc atgtttgcgg acacgatggg gatttgcagt gtgatttcct tcgtcaactt
    47581 cgtttgcgtg gaaagtttgc ggcgcgaatc cacatccgaa tattgcggca cgagcaaaac
    47641 atcgtcatag gtcagggcgg tatcttgcaa aatcttcata attggctcct ctactccaaa
    47701 atcaaaccgc ccgaattata atgcttgact gttggtaatt ggtaattggg aattaaagtt
    47761 tcagtggagg ggatgattct tgaaacggga tcaggttgca ggtcaggtgc gaagcgttgt
    47821 tgaagcgtgg atcatgttcg agcgttggaa ttcaaatcaa ggagtcaaat catgacggtg
    47881 acagcagtga tcggttcaca gtggggtgat gagggcaagg gcaaggtggt ggattatctc
    47941 gcggagcagg tggattatgt ggcgcgattc aatggaggga acaatgcggg gcataccgtc
    48001 atcaacgagt ttgggacgtt caaaattcac ctggtgcctt cgggaatttt tgcaaagaat
    48061 acggtcggtt tgatcggcgg cggtgtggtg atcgaccctg cggtgttgat cgaagaaatt
    48121 gacatgctca acaaagcggg cgtgggtgtg gatggccgtt tttggatctc gccgcgttcg
    48181 catgtgatca tgccgtatca taaaattttg gatggcttgt acgaagaggc aaaaggcgcg
    48241 ggcgcaactg gcaccacgcg gcgcggaatt ggccccgtgt ttgccgacaa ggtcagttac
    48301 aacggcatcc gctggacgga ttttgcgagc gacgcattcg agaaccgctt gaaggtgcaa
    48361 ctggaattaa agaataagat catcgttgcg ctgggcggcg gggcattgaa ttatgaaagc
    48421 gtgcgcgatg agtatcgcgg atattattcg aagttgaagc catacatcaa ggaattgttt
    48481 tcgctggtgc aggatggatt gaaagcgaat aaacattttc tgcttgaaca ggcgatggga
    48541 acgtttttgg atacggactg gggaacctac ccgttcgtga ccgcttcgac gacgattccc
    48601 tcggcggctt cggcggggct gggaattccg ccgcgctata ttacgaatgt agtgggcgtg
    48661 acaaaggctt acaccacacg cgttggtgcg gggccattgc ccacggaaat tctcgatacc
    48721 aaaagcgcgg agtacgaaca attcggtgaa gtcgccgcaa cgacggggcg aatccgccgc
    48781 gtgggctggc tggatctgga aatcgtgaaa accgccgccc aactcagcgg cgtgacagaa
    48841 ctctgcctga caaaactgga caccctcagc gggctggaga atttgcaagt ctgtatcggt
    48901 tacaagctaa acggcatgga tgtccgctat gcggatgtgg atgcgtatgg gctggatcaa
    48961 gtggaaaagg tctatcgcac cgtggcaggc tggaaggaag atatcagcaa ggcgcggtca
    49021 ttcgaggatt tgccagtgaa tgcccaaaat tatgtaaaga tgatcgaaga ggcttcgggc
    49081 gtacccgtca ggtggatcgg ggtggggcct gagagggatg cgacgataag aattaagcag
    49141 gattcctaaa tctggaacac atgccctgta tgctatactc gccgtatggc aaaacacaaa
    49201 cctaaaccga tcaaattcag ctggg
//
LOCUS       DAOQLC010000012        48740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_186_length_48740_cov_21.839827, whole genome shotgun sequence.
ACCESSION   DAOQLC010000012 DAOQLC010000000
VERSION     DAOQLC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 48740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186_length_48740_cov_21.839827"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..1236
                     /locus_tag="PL000_03705"
     CDS             337..1236
                     /locus_tag="PL000_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNC88029.1"
                     /translation="MDFSWISNPESWIALVTLIALELVLGVDNVIFISILSGKLPQED
                     QQRARTTGISLAVITRVLLLLSLSWIIGLTEPLFHLPFFEGEVAEITGRDLILLLGGL
                     FLLWKSTHEIHDKLEGKEGSASAKVAANFVSVIIQIMLLDIVFSLDSVITAVGIADNI
                     IIMIVAVVAAAVVMIFTAKPIGDFVERHPTIKILALSFLLMIGFTLIAEGFGVEIPKG
                     YVYFAMGFSVMVEMLNLRVRDSHVKPVNLRDPYKEEKVELQPAFVAASKAATVTTEKL
                     TTSPAKKASTKTKAKPKSKTKKS"
     gene            complement(1279..2163)
                     /gene="thrB"
                     /locus_tag="PL000_03710"
     CDS             complement(1279..2163)
                     /gene="thrB"
                     /locus_tag="PL000_03710"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC88030.1"
                     /translation="MLKIRVPATSANLGPGFDCLGLALDLWNEITFEPAEKLTYHVTG
                     EGAEKLNKGTKNLLTKSYSNLYEFCGKKVSPVRICAHNEILMSSGLGSSAAAIVSGLF
                     GANEMLGSPLSQNELLKFASDMEGHPDNVAPALFGGLVVSVMAGEEIISHRYEVPELT
                     IVIVKPIVEWLTKTARAVLPKSVSRADAVYNIGRTSLVVEALRTGNLALLQKVMDDRI
                     HQPYRLRHISGGTAAYKKARQFGAAALSGAGPSIITFVPPDHAELARSEIESVFVERG
                     IETKSVITKPGKQGAHRV"
     gene            2260..3294
                     /gene="thrC"
                     /locus_tag="PL000_03715"
     CDS             2260..3294
                     /gene="thrC"
                     /locus_tag="PL000_03715"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC88031.1"
                     /translation="MTYQGLIHRYGQYLDLPAHTKTISLLEGNTPLIPMPRLGRELGC
                     ELFIKFEGLNPTGSFKDRGMTAAISEAVGRGAQTVICASTGNTAASAAAYAARAGLRS
                     IVLIPEGKVAVGKLAGAIAYGAEVIQIQGSFDDALTLVVEITQKTPIALVNSINPFRI
                     EGQKTASFEICDDLGSAPDWLCLPVGNAGNITSYWAGFKQYKKGLPQVLGVQAAGAAP
                     LVLGHPIEKPETIATAIRIGKPARGEQALQAAEESNGRIIAVSDEQILSTQKILAAEG
                     VWVEPASAAGLAGLLAESASGKFEAKGKRIVVVCTGHGMKDPSIITESFKSPKVISAD
                     YDALISAVGA"
     gene            3382..4386
                     /locus_tag="PL000_03720"
     CDS             3382..4386
                     /locus_tag="PL000_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88032.1"
                     /translation="MFFNEWNILVVDDEPDVLAVTKLALRDVKVYGLPVKIHTASSKA
                     EAIEMLKGPLALQGSTEPIVAVALVDVVMENDQAGLELCKFIREEANGHSVQLFIRTG
                     QPGVAPERSVIDNYDISGYFTKVELSEQKLYTLVKSGVRQWFTSWYALMTEESTNNIV
                     THSGSRKDFLENGIGWFGEPGPQEGEGVTGFIFDRNLIVSDYPTQIKPLYDRLNALKP
                     VIETAEGHKLAMDEMGNLLVKTVKTKTTADYAYVGESSMVMPRLLLELTFKSGLVFST
                     LWKKITDDEKRAAAKKPVAKKSAKKPAKPAKKAVKKVAKKVAAKPAKKTTKKAAKKKK
                     "
     gene            4491..5699
                     /locus_tag="PL000_03725"
     CDS             4491..5699
                     /locus_tag="PL000_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88033.1"
                     /translation="MTKKNPVLNPNILQKFSFFASFSKDQLEEIAKSAPKVACKTGEI
                     VFRQGDRSATMYLILKGAVKIEREDSVGGIVSVGQLSVHQVFGELAMLSKEPRQATVT
                     VTKNAEFLVIDRKMMLEIIAKSDPEEIIEVFSVLSEQTRAANDREFHETLSRRTLEAQ
                     MEVEKQRALTQLVAGVAHEINTPLGIINTAVSIMARELSEPSEITAQRAADIAESLEL
                     MRRNVERAHRLVQDFKKVSASQLMDEIETFDISETIRETVDLMMVGLKSSQIQAKFHD
                     KLTLTQKAWMGYRGGLSQILINLLTNAERHAYPNGAGGLIDITIEMFDEKNYRLTVQD
                     YGQGIPAENQPRIFEPFFTTARARGGTGLGLAIIHNLVVNVLKGEIQLRSEIQKGTAF
                     LVVFPRNLPK"
     gene            5817..7460
                     /locus_tag="PL000_03730"
     CDS             5817..7460
                     /locus_tag="PL000_03730"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88034.1"
                     /translation="MNETPLVVENLTFKYRVRPELAIENISFELKQGELLLIAGSSGC
                     GKTTLARCINGLVPRSYRGERSGRVLLHGKEVTELKIAEMAQVVGTLLQDPERQIVAS
                     NVYNEIAFGPENLGLPHDEIETRIEQALKRLKIEYLRDRETFNLSGGEKQKVALAGLL
                     AMNPSILLLDEPLASLDPASAHDALKIFRSLADEGKTIILVEHRVEDSIEAKPDRLLY
                     MESGRIKYLGGITSLPKEIDHTQVKLPAQWVVQRVKEDKKHADVPPVQKGESGDEPLV
                     VFEDVSFRYDDEYPLILKDVNLTINRGDLIAVLGPNGAGKSTLVKHAIGLLKPTSGRV
                     LVQGEDTRKMSVAQIARVLGFVFQSPTHMLYAPTVQEELEFGPKNLAFDKEAMDKSVT
                     ESLNAVNLKGFEEYPPLGLSFGQQKRTTIAAVLAMQSKIMVMDEPTAGQDFANYTRFM
                     DALCSASNNAKSILSANFDSTLFITHDVDLAVTYANRVLLFGDKHVVADGKPEEVLKD
                     IDLLMKYRVRPTSLLHLNLDLFAKTKKFLPAEALAGFVS"
     gene            7546..8094
                     /locus_tag="PL000_03735"
     CDS             7546..8094
                     /locus_tag="PL000_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNC88035.1"
                     /translation="MNDKSTWEFGTRQVVYGAIGAALYGVLSLATNYFPLPAAGNVTF
                     RPAVAVLIFFGAAYGPWVGLLAGFIGNTLGDAAMGWGFYWNWSLGNGLMGLVAGLAMP
                     MLKDFKARGDIIKAIIYGLIGIAVGMLFASVTEKFIGGLDWNTALVGYFTPAFIGNAV
                     VVVILLPVLMIAFAAVASRRGR"
     gene            8100..8993
                     /locus_tag="PL000_03740"
     CDS             8100..8993
                     /locus_tag="PL000_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNC88036.1"
                     /translation="MLVTWKYRPRDTFIQRLDPRTRFIYMVCIIFALTLPDIWDYRII
                     FPLFISSLTLYLLARIEWKDVRRAWIFIGVFVFFIVGLNALLSGRGGPDTVTNVYSPT
                     IFNLPLKIPFTNIGWDIPVTVAKLWFAVNQITRMLTMAILAFPIPYTVSPTIYGTTFK
                     RMGTSDKFSFTMDLAFRFLPTFARDLMITMDAQRARGYELDKLKGGIAARLRKLAPLI
                     IPVVMQSTVTGEEVIDAMDLRAFGTAPRTWLQSQDLQYKPMDYVMLSLGLAILVVCCI
                     LKWGFGIGGFFVPDFFMNLFV"
     gene            complement(9050..9877)
                     /locus_tag="PL000_03745"
     CDS             complement(9050..9877)
                     /locus_tag="PL000_03745"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNC88037.1"
                     /translation="MTTKTYRQLLNEWRKERDETIRRENGWLSLAGLYWLKLGKNQFG
                     ADPKCEIQLPARVSPVVGYLEYNGKSVSMRTTDGHKVHVNEKLSDFAILHPDISENPS
                     FIKFEDICLVVIQRGNRLGVRMWDNQREERRSFPARSWYDIDEQYRIPATFTPYDRPK
                     MAYFPDITGEKSEFPVEGYLSFEFGGNHYQLDINKEDDGTIFVRFWDPTSKDETYPTG
                     RYLIADIEADGKIFIDFNRAYSPPCAFTDFATCVFAPEQNHLDFKVLAGETYPRHEN"
     gene            complement(9946..10539)
                     /locus_tag="PL000_03750"
     CDS             complement(9946..10539)
                     /locus_tag="PL000_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88038.1"
                     /translation="MKKAFGFLSNEIFLGTMIALLSAFTAIASYQGAMSDSEQNKYEI
                     LGMQTLNDGNAEYLRANQDITQDYNYFDNWYINAGERPEIAEYYQTSFSEQLAAAIER
                     DPETVWDEQYYTDMYTEATVLWDESDTNFDLASQWDERGDQLQLVMLIMALGLALAAW
                     ASLLGAESTMRTLFSLFGLLAFIAGLFIYLTMVPTVG"
     gene            10713..11267
                     /gene="dcd"
                     /locus_tag="PL000_03755"
     CDS             10713..11267
                     /gene="dcd"
                     /locus_tag="PL000_03755"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNC88039.1"
                     /translation="MGLKPDKWIRKMALEQKMIEPFVDSQVREGVISYGVSSYGYDVR
                     VANEFKIFTNVFSATVDPKQFDTKSMVDFVGDVCIVPPNSFALARTVEYFRIPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGLAQVLFFEADE
                     ECDVSYADKKGKYQKQQSIVLPKL"
     gene            11320..12591
                     /locus_tag="PL000_03760"
     CDS             11320..12591
                     /locus_tag="PL000_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNC88040.1"
                     /translation="MADSNEAQTMQKQMDDMNKMLLRVISHLARSTDGQTSLILRDLT
                     QISSTMAALKQSITGYLSTYERQVGALVGVGSAINSSLGLKRVLEMVMDSLIALMGAE
                     RGLLMLREPNGELKVRVARGGDREDLAQEVFSISNTVVQQVAETGNAILTTNAQDDPR
                     FGRQVSVAAYRLRSILCAPLKIKDNLIGVIYVDNRAHTGIFGNNDLSLISAFSNQAAV
                     AIDNASLFDDLQESNAELEIAYQATLEGWVRALDLRDKETEGHTQRVTALTQRLAQAM
                     GVDDDALLHITRGALLHDIGKMAIPDGILLKPGSLTDEERAHIQKHPVYAYEMLSPIK
                     FLHPALDIPYCHHEKWDGTGYPRKLKGEEIPFAARIFSVVDVWDALISDRPYRKGLPP
                     SEIRERIQKDSGSHFDPKVVETFLSLDNLAV"
     gene            complement(12700..15498)
                     /locus_tag="PL000_03765"
     CDS             complement(12700..15498)
                     /locus_tag="PL000_03765"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNC88041.1"
                     /translation="MTSIVSLDIETTGLDENREAIIEIAAVKFNGRRIESEFNTLINP
                     GRHIPDFITGLTGIDDAMVRQAPRLRDIANDLLAFVGDAPILGHNVKFDIGFLRKAGL
                     FEYQQTIDTYELASVLMPTASRYNLGSLGQQLNILLPATHRALDDARVTQAAYVRLFE
                     MASELPLETLNEIVQLGDFVDWDAGWVFQQALKQRAKEAPKAKRARLASFSGPMFDSA
                     KRESASIQKTEEPLPLDPEEIAAVLEYGGPFSQYFESYEHRPEQVEMLKAVTNALSTG
                     NHLMVEAGTGVGKSFAYLVPAAYFAVQNSTRVVVSTNTINLQDQLIKKDIPNLKAALN
                     LENLNATVMKGRGNYLCPRKLEYMRSHGPKDANEMRVLAKIMVWMLDNASGDRNELNL
                     TGPTEREAWARLSAEDDNCTTETCMGRMGGSCPFQRAKQAALNSHILIVNHALLLSDV
                     ASGSKVLPEYDYVIIDEAHHMESAVTGALSFRMTQNDLERMMKELGGSSAGILGRILT
                     DLHASLRPSDFGLLQQKIKRATDQAFRIEQLSKQFFMNLSDFIAIQREGQPQSNYSWQ
                     MRVMPATRTLPGWDDVEMGWGQVGETMSLLLKTLDELYKGLGDLMSEGHDSVEDIMGS
                     LSSLMRRMTEAEAASSGMMHKPSNELIYWVEVNPRGERLSLNAAPLRVGPLMQKHLWH
                     EKASVIMASATLTTHGEFQYIRNTLSAEEVDTVALGSPFDYESSTLLYVANDIPEPNI
                     HGYQQMLDKTIIATAKATGGRMLVLFTSYAALKKTAQAITGPLAREDIFVFEQGEGAS
                     PNALLESFKSTDRAVLLGTRSFWEGVDVPGDALSVVFITKLPFDVPSDPIIAARSELY
                     EDSFNEYYLPESILKFRQGFGRLIRSASDRGIVAILDRRVLTKQYGRLFLESLPQCTA
                     RQGAAAGLAKMAGQWLGT"
     gene            complement(15607..16956)
                     /locus_tag="PL000_03770"
     CDS             complement(15607..16956)
                     /locus_tag="PL000_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88042.1"
                     /translation="MKVSEKIGVTLLIWVILAGMAFFRLKAYGSINLSVANADTPSYI
                     DEAAPPLFSRDMLTRSRLFTTNLVYHLAKVQECELTAISHPALGLETERAVQPCFTRI
                     ALLQNLLSILAWSLLALTVASKLTGGFEKLFAAISITAFGFTPAIADWDSILGSESLT
                     FSLFAISLALLLKICFTAIEEDQRPTSILLTILTLVSLALWTFTRDSNVYALAVLFAF
                     LAVSLVIFPRLRKNKSVLVISAVILVLTVVGLQSAMESRRWKKPLTNVFAEFILPYPA
                     RAEFMTGIGMPDPKSAEYPTWFRENAPRAYARFLLAHPGYTLTSFISNLGGIFSENTQ
                     PYFFSEQTGTRKVLNALNDILHPNTHLVFILDLLLLMGLAVSAIARRNGEFGAWAWLS
                     LWLIASASAMMVINFFADSIGVTRHTMLAVEIFRLMLWIFLAILFDQANRPLKSEKG"
     gene            complement(16981..17673)
                     /locus_tag="PL000_03775"
     CDS             complement(16981..17673)
                     /locus_tag="PL000_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88043.1"
                     /translation="MKIIKNEKLIERNGKIGQWMSLASLIVLGLGLYISFTVPEYFIY
                     SIVCLVIGFTMTQISIYMGNRWGRSPRADEKFDAGLKGLHSDFVIYHFSSPVSHLLIG
                     PSGAWVLHPLHQRGKISYQKNRWKLSNGGFLQAYMSVFGQEGLGRPEIDAENEVQALR
                     KFLAKKMDESTIPEIKPILVFTNDEIEVDAGDSPIAAMRLKQLKEFMRQGGKNRSLSN
                     DQIKALSDALES"
     gene            complement(17674..18690)
                     /gene="plsX"
                     /locus_tag="PL000_03780"
     CDS             complement(17674..18690)
                     /gene="plsX"
                     /locus_tag="PL000_03780"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNC88044.1"
                     /translation="MARIVVDAMGSDSYPVPDVEGAVMAAREYGAEIILVGDEARIKP
                     VLATQNTEGLKIRIVNTPEMLTMEDKGENLVLKARAKDAKTSMAVGIDLVKNGEADAF
                     VTAGNTGAGMVTALFRLGRIRGVDRPALAPIFPTATGTCVVLDIGANPDCKAENLYQF
                     GILGSIYAEKVRGVKSPKVGLVSNGEEEGKGNELVKAATPLFKASKLNYFGNVEGKEV
                     IGGKVDVAVTDGFTGNVMLKSSEAVAKLITDKIREIIKNGSILTKIGGMLVRPALGAI
                     KKLLDPSEQGAAPLLGVNGLVFIGHGRSDAIAIKNAVRVAKHAVDVKVLDAMKSAIEE
                     SLKS"
     gene            18756..18837
                     /locus_tag="PL000_03785"
     tRNA            18756..18837
                     /locus_tag="PL000_03785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18790..18792,aa:Leu,seq:gag)
     gene            complement(18874..20199)
                     /locus_tag="PL000_03790"
     CDS             complement(18874..20199)
                     /locus_tag="PL000_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88045.1"
                     /translation="MDDKSGYKEPLSSQLRRRVYGNEEQIAETVRWVAYSSLFILLVL
                     VINQLLKGRYIQTLVILAGTLPIIISLLMIKSGKLSLPISIFATGTIILLTYISTVGN
                     GIHDVANMAFPIILIVTGLILRGRVLPYLTGGIILCLGWLVFGDMLGLYKADTELASL
                     PEDFLFASAIILVTSNAVHLLVENIRRSLIRAREEIAEREKAEMEREELVRQLKLKNQ
                     ELDRFAITVSHDLKTPLITITGYLGYLERDALAGNMDRVHKDISQVNEAAKNMGKLVD
                     EILDLSRIGRIMNPPREVDFAEIVGEALKASSGLLQEKQVRVKVGSEFPIVYCDFARI
                     VQVIQNLVTNAAKFMGEQPVPVIEIDTEVRNNERVFFVRDNGMGIDPKYHEQIFGLFN
                     KLNAKADGLGIGLGIVKRIIEVHEGRVWVESETGNGASFYFTLPDKPLL"
     gene            complement(20215..21864)
                     /locus_tag="PL000_03795"
     CDS             complement(20215..21864)
                     /locus_tag="PL000_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88046.1"
                     /translation="MKKTKSASDEKQNAPEEKLPRRSDVLLDAIAQSAVRLLEAPDWK
                     TEINDLLKLLGEATHASHAYLFENHLDENGNLVTSQKYEWTGPGQTSELDNPDFQNVP
                     VLEEGLAAWYSVVSQGKPFYNTTKIFEEVWQDTPSRQDIKTLLDVPIFVDGKWWGVIG
                     FDDCIREMAWTKAEVHAMQVTASMLGSAIKRQKIDTALRASEEKFQTTFHHTFVPMVI
                     GRLSDKSIVDINEAFSQEIGFSREEAINHKASDLNLWADPDEHNKHHQLLQEQGFIRE
                     FKARYRRKSGEAGVALLSVAPITVNNEACVLYTLYDITKIDELLIELQNKNNELESFT
                     YTVSHDLKAPLITIGGFVGYLEKDILNGNTAKAQKDIQRVMDAVIKMQRLLNELLELS
                     RIGRFVNKPENANIGDIAREASRLLHEQVTSNKVKVIIEKDLPSMEVDRHRLVEVYQN
                     LIDNAIKFMGNRSNPEVKVGSFQQENEQIFFVKDNGIGIDPLYHERIFGLFNKLDAGT
                     DGTGIGLALVKRIIEFHGGRVWVESESGKGTTFCFTLTART"
     gene            complement(21947..22705)
                     /locus_tag="PL000_03800"
     CDS             complement(21947..22705)
                     /locus_tag="PL000_03800"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNC88047.1"
                     /translation="MPIAITRKISPRFNECEVTHIERTPIDLNVARAQHQEYVHALAA
                     LGCQVIELPEEPDLPDSVFVEDTAFILPEVAVITRPGADSRKPETESIIRALSSYRPL
                     VHVTEPGTVDGGDVLVLGKNIYIGNSTRSNADAVRQIQQLLDHYGYTVTAVDMHDCLH
                     LKTALTKVDDKTLLINPNWVDTSHFKGFDWIEVDSAEPFAANCLPVGDGIIFPTAFPK
                     TSNKLEARGYKIQAVNVAELAKAEGAVTCCSLIV"
     gene            complement(22706..23458)
                     /locus_tag="PL000_03805"
     CDS             complement(22706..23458)
                     /locus_tag="PL000_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC88048.1"
                     /translation="MVKISNLDKYFGRLHVLKNVSLEVPERQVVCLIGRSGSGKSTLL
                     RCLNFLEEPSHGTIEVDGVRVEGGEKGSRHRQLVHDVRLKTGMVFQEFNLFPHMTVLE
                     NVIEGPVIVKGMDRKKAIELGEQNLDSVGMLFKKDEYPMRLSGGQKQRVAIARALTME
                     PRVMLFDEPTSALDPELIGEVLNVMKKVALEGMTMIVVTHEMGFAREVADRVIVMAEG
                     ELIEDATPDDLFKNPKDPRTKALIDRYRSGEN"
     gene            complement(23472..24290)
                     /locus_tag="PL000_03810"
     CDS             complement(23472..24290)
                     /locus_tag="PL000_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HNC88049.1"
                     /translation="MNLFAKAESGAPKTQAELLFEAQQRKERHFRANVALTWGVLLLL
                     LVFLFSGPINVDTNFIKENMLFIAGGVGETIRISILSIILATFLALLAALGRLSTFPP
                     IYALSTFYVSLIRGTPLYLQIMFFFLALPQLDIRLPGLWAGVIALGLNYGAYMSEIFR
                     AGLASVGKGQREAAAALGMTPGQTMKRVILPQAMRFAIPPTGNEFIAMTKDSALVSAT
                     GFVHELTWRAVKVGRAQFHNLEALIMAAIFYWVMTIILSYVQGVIETRLSKGDR"
     gene            complement(24364..25266)
                     /locus_tag="PL000_03815"
     CDS             complement(24364..25266)
                     /locus_tag="PL000_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNC88050.1"
                     /translation="MKKLLTMASLLVVASLMLTACGGGGSTATDLLGAIKERGYILIS
                     TDPNYEPQSFLNTEGSRPSDTKCPSDALTTAEMQGFDVDVAIAVGDALGVETCFATPG
                     WDTLTAGNWADKWDMSVGSMTITTDRQKVLDFSVPYYYTPAVVAVLGDSEFQALEDLS
                     GQAICAGVSTTYETWLNGGEIGLPASSIYAKPPSGITVVPLESDQECAQALAAGRTDF
                     AAYSTSATVVNANIAAGIPVRVLGSAVYSEDLAAAFDKSSTLPTASLIAEVNKIFEAM
                     HGDGRLTELSMKWFSEDLTQAPNK"
     gene            25424..25834
                     /locus_tag="PL000_03820"
     CDS             25424..25834
                     /locus_tag="PL000_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNC88051.1"
                     /translation="MSVIFPSAEWLAELEAKLNSDEKYREIAKNWEGDIFFNIEPEGN
                     LKEPLTFYLDLWHGACRKVDYNPAAATYPKPTFILNSSYGNITAVLLGKMNPMTAMMT
                     MKLKVQGNMGYMMRNVPTVLDFVRCAQEVTTDIL"
     gene            25891..27702
                     /locus_tag="PL000_03825"
     CDS             25891..27702
                     /locus_tag="PL000_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNC88052.1"
                     /translation="MLPILKINLTTNTTEEFVTPKGWERDFLGGASLAARILYSTLTK
                     ELDPLSPESPLLFINGPLTGTNGPTTGRFVICGKSPATQLWAESHIGGFWGPELRKAG
                     YDGLWITGKAQSPIYLWIEEGRFEVRDAARVWGKDVYEAQEIVKEEVGVKAARVAVIG
                     EAGERGVYYSSICCDHGRMAGRTGLGAVMGSKNLKAVAVHGKNKIEVTAQYAQARSEA
                     NRTLKQDNEAKILHELGSSGVANYAEYLGSMPAKYYHKGSFEEVDHISGSKMAESILV
                     STSACQGCVIACGRVVNLGDGAKRKGPEYETIAGFGPNLLIGDLNDVVRLGEMCDKYG
                     MDAISASNTIGLAFHLFEMGKITLQDTGGLDLTWGSFLAARQLLRMIARREGIGDLMA
                     QGSRALGKAFDAEDEAIQVNGLETAYHDPRGVSGMALSYATSPRGACHNQSDYFFVDF
                     GHTYDEIGITYFDRHAQAEKAANVAKHQDWRTHFNAVLICIFANVPPETQVQLLNAQL
                     DLNWTIADLMRSGERGWNLKRVINNRMGLTRANDKLPKAFLEPHQEGGAAGFVPDIEG
                     MLSAYYETRGWDVRTGRPTKQKLIELGMSDIANDLWG"
     gene            complement(27726..28922)
                     /locus_tag="PL000_03830"
     CDS             complement(27726..28922)
                     /locus_tag="PL000_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88053.1"
                     /translation="MIRILDIALKDLLQLMRDFKTFMFLLIMPILFTFLFGYAFGGFG
                     GNESDSRVPVGYLSLDDSWVTEELHDLLKNSEVIRLDENVFRSASDLEKLVANGELAA
                     AIIVPDGYGRAVLKDKTIRLTVLADTNTQAWMTIEAEIFTAASRVDGAVRTAGIMEQF
                     KMEGVPFNYAFDEILAAWDEPPIAIEETSSLVIKEGEENNALAHTSPGMMLQFAIAGL
                     LTAAQVIVTERKSRTLQRLLTTDTRRIQIIVGHFLAIFSLIFTQFLILICFGQFALKV
                     DYLRLPSATLLVAFTAALCISALGLLIGILARTEEQAITFSLVPMFVFSGLGGAWVPL
                     EFTGETFQAIGHISPVAWGMDGFENIVARGLGFESVVIPCLALAGYALVFFVLAVWRL
                     NATEEK"
     gene            complement(28932..30236)
                     /locus_tag="PL000_03835"
     CDS             complement(28932..30236)
                     /locus_tag="PL000_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88054.1"
                     /translation="MKAFDIALKDMTRSFRSTFAVVFMFGVPLLVTGMFYFMFGNIAD
                     EGGFTLPKTNVIIANLDEGGPKFQVNPKNIPGGKEADSMGDLIVSILQSDDMADLIEV
                     SLAPNAESARSAVDRQESQVAIIIPADFSHQFADVDGKAVIEFYQDPTLTLGPAVMRS
                     ILNRFMDGMSGVKIAINFFMDEAQPEQLVLTGQVVDQYLEVSLAESDDPEAELLEVRN
                     PANGPEKEADESQNLLLGIIGPIMGGMMVFYAYYTGTSTAQSILREEEERTLPRLFTT
                     PTSQATILTGKLLSVFMTVSVQIIVLLIAANLIFGIQWGEFTSVALMAVGIIVSASSF
                     GIFVNSFLKDTKQGGVLFGGILTVTGMMGMIKIFAMNSAIANKMGDTISLLVPQGWAV
                     RGLMQAMSGESFNSIALTVLVSFLWGAAFFAIGVWRFNKRYA"
     gene            complement(30248..31195)
                     /locus_tag="PL000_03840"
     CDS             complement(30248..31195)
                     /locus_tag="PL000_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88055.1"
                     /translation="MNTIVPAIEVQGLRKSFGENHAVQGVSFKVDQGEIFSLLGPNGA
                     GKTTTISMLSCLLHPDEGDAQIMGHSISTNQRGVKSVLGVVPQDIALYEDLTARENLV
                     FWGKMYGLRGTALKSRVDEVLDVIGLRDRASERVGKYSGGMKRRVNIGVALLHKPKVI
                     YMDEPTVGIDPQSRRNILDSVILLKNEGMTVLYTTHYMEEAQEISDHIGIMDHGQLIA
                     HGTHDELVKMVGQQTRIDLTLNGNMDTVIPAWKELADVSRITLNGELISVLVQDSNLT
                     LPHLFETATKYSARITSVNISEPNLEAVFLHLTGRALRD"
     gene            complement(31334..31765)
                     /locus_tag="PL000_03845"
     CDS             complement(31334..31765)
                     /locus_tag="PL000_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88056.1"
                     /translation="MQELYYIITISSGIKTGREAAMNWKMYYDEAEKIIFIKTNGVLE
                     IKSATLMRNEGVELIRKHNCMRCLLDHRDISEDALATMDIYELPKRYAELGIPRQFKI
                     ALIVPKKLETNLKFYETVCRNNGFFASLFVDYESALDWLKK"
     gene            31894..33435
                     /locus_tag="PL000_03850"
     CDS             31894..33435
                     /locus_tag="PL000_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNC88057.1"
                     /translation="MKLVTVAQMRAIEKEADANGVSYAQLMQNAGRGLAEVVHALGQE
                     NGWEEVTALVGAGNNGGDALVALTWLAQAGWTTHAYLVRRDSANDDLVTAYLAAGGEV
                     ADFEDDRALAVFLNESDVLLDGLLGTGIRLPLKKEAALVLSNAKEMISEMEIPPFVVA
                     VDCPSGVDCDSGEIAEETLPADLTLTMAAAKYGLLKLPAFEFIGELQVVDIGLPDGLS
                     SWQEVNTEAADDELVAVLLPERTPASHKGTFGTAFVVAGSTSYSGAALLAATAAYRIG
                     AGLVTLAVPEPLHIALAGRLLEATWVLLPHENGAISEKARDPVLKNLERATSVLIGPG
                     LGDFASTGKFVAGILPAIKLPMVIDADGLRHIARIKDWHKKLFDVAVLTPHPGEMSAL
                     TGLSTEKIQGNREEVAVSYAKMWGHVVVLKGAFTIVAAPDGQRTVIPVATPALARAGT
                     GDVLAGLVAGLRAQGLSAYDAAVAGAYIHARAGILAADTLGTTASVLAGDVLDMVSDV
                     LAELE"
     gene            complement(33553..34593)
                     /locus_tag="PL000_03855"
     CDS             complement(33553..34593)
                     /locus_tag="PL000_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC88058.1"
                     /translation="MARIFDVIEYPNEMNDEIVHRFPEEGFGDFRIGSQVIVRESQSA
                     VFFRDGQALDVFKPGRHTIATANIPQLIDFIGKAFNDRTPFPAEVYFVSRKEFANKKW
                     GTSSSILVPTPGTGLGWQVLTGYGTYSFQIKDPQQFVTQIVGATGSYRSSEIEGRLRD
                     MLVSKLGDLLGETAQTTNLTVKLSSLFEELSAGVRAKAKDDFEAIGLTLKTFYIGSLK
                     PSERAVQELRERAVLDSNLYAQLQAADAMREAANNANGGAGLTAGIGAGMGIGNLMGQ
                     ALQGGMQNTSGGGAAGAKIADIMTPAEAAQVMKVTEDDILEAIKDGSLKAKKVGKAYR
                     ISKDNLESFMNS"
     gene            complement(34761..35570)
                     /locus_tag="PL000_03860"
     CDS             complement(34761..35570)
                     /locus_tag="PL000_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HNC88059.1"
                     /translation="MTASIHQALFELEKNNGSAALCTVVKSEGSTPRHVGSKMLVYPD
                     GNFIGTVGGGDLEHRVLDEAWMALGDGKPRYLHYNMADPSRGDPGVCGGQVEVFVEPI
                     LPPPLLVVIGAGHVGKAVVHLAKWLGFRVAVCDDRAEFCTPEATPGADIYHPITMDKL
                     PEHIKIDSRTVLILTTRGSSVDAVGMKPLLDSTAGYIGVIGSRRRWATTVKALKEKGV
                     SEEKIARVHSPIGLELQAETPEEIAVSILAEVMMVKAQATGMPMKAKQATG"
     gene            complement(35571..36029)
                     /locus_tag="PL000_03865"
     CDS             complement(35571..36029)
                     /locus_tag="PL000_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88060.1"
                     /translation="MIQKPDVFSILKNDSATASSMGGLIFTIAVTGIIYVTKALTGIV
                     PANVNPLNFIWLTLAALIYSIIVIAVRVPYVTATFEHGIEVNAQILKSSVFRTVWTLH
                     LHYIHLGQTHDVKLKQLITEKTKLMLEKKELALIVDQRNPKNILIRDVYL"
     gene            complement(36029..37372)
                     /locus_tag="PL000_03870"
     CDS             complement(36029..37372)
                     /locus_tag="PL000_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNC88061.1"
                     /translation="MPDQIYVIGHVNPDTDSIAAAMGYAWLLRERDGANTIAARAGAL
                     NAQTSWVLKTVGLETPVLLTDASPRFESVMRRLDTIRPDSQLGMAWTLASKTGGVAPV
                     VNEDGKPFGIINGMSLFKYFSETLGPRPGDTTVREMMAAQCKDAADTSVPKYAANAHI
                     RDALNKILRDEFNDYWVVDEHGVYVGIANQREVLNPPRLKIILVDHNEPRQAIAALEE
                     AELLEILDHHRLGNPYTHQPIRFTVDTVGSTSTLVAEMTAEAGLSMPPALAGTLLAGL
                     CSDTLILTSPTTTQRDKVAADRLSRWAFVGGSPLKGETIESYGKAVLSAGAGLSNRDP
                     KDVVSTDIKPFEGGGFKFAVAQAEVTDILQLTEHLEPLQNALNDLRDKRGLHFAMLLV
                     TDVVRGTSRLLISANAPTILEDLPYPPLSDGTRDAQGVVSRKKQLLPAVLGLLER"
     gene            complement(37473..39143)
                     /locus_tag="PL000_03875"
     CDS             complement(37473..39143)
                     /locus_tag="PL000_03875"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC88062.1"
                     /translation="MTRSEIPHLIPYLVSLAVSIAVFVYTWSRRKARGAFAFSFYGLG
                     QSLWLTGFICELTSSDISTKIFWDSFQWFAGFFILISLPIFLVQYTEYKLNYPRLLFG
                     LSFIVPILFSSVLFLDQNLHWVYRNPHLSTQGFFTELLYDFTPLVYGYAFYALLILAW
                     GLRILFQRITHSHSLYQTQMIMISLGLLIPILGMALTLFDIRITPQRDMAPFTFVIGN
                     LFVVWGLFQFRMFNVTPIARDTVFEAMVEPVVILNNQNDIVDINLSMLALLGKTADDV
                     IGEPAKKIFADFPIPIKQYMQTSYARAEATFDLGGVHVYYELTVWPLYNSNKEMTGRI
                     YISHDITAQKELERELRKLNAELEDRVRARTWELADAYDTTLEGWARALELRDKETEG
                     HSRRVTDMTLKIAVAMGVAGDDLEHMRRGAILHDIGKMAVPDEILHKPDKLTDNEREI
                     IKKHPEIATKLLSPIPFLKKALDIPYSHHEKWDGSGYPQGLKGEEIPIAARIFAVADV
                     WDAVTSDRPYNKAWPREQALKYFGEQTGKHFDPRVVNIFLKLVEQGEI"
     gene            complement(39275..42244)
                     /locus_tag="PL000_03880"
     CDS             complement(39275..42244)
                     /locus_tag="PL000_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC88063.1"
                     /translation="MNITLKVNGIEHAIDTAPATTLLAALRGLGYHGVKFGDEGNLSG
                     ADTVLLNGKPVNAGALLAAQAEGHDIATIEALGEHPEQGWKTTDGLHPLQKAFVETGA
                     IQCGYCTPAQILAAKALLEKNSNPSEAEVREAISGVLCRCTGYLKPVQAVMKAAAEMR
                     GDTPLDWNSINWDFGTPKDDSPQNDAPASNLTGVQVRPKVVVTPETQTWQTVGKPEIK
                     LDAVKLVQGKPAFAADFEKRGLLHAKVLHSPHAHALIKNIDASKALALEGVAAVLTWK
                     DLPRVVYSTAGQSDPIPGPLDAFSLDKKVRFVGDRVAFVAAESPEIAEKALSLIEVEY
                     EVLTPILDSRQAMGSPIRIHDEPEYVNFADSNPEKNLAAHIRIDIGDVEKGFAEADEI
                     FEAEYEVPKVQQAHIEPHVCVTYWDEDDRLVIRTSTQVPFHVRRMLAPVLGLPVKRIR
                     VIKPRIGGGFGGKQEVLMEDVAAHLTIATKRPVIYEYTREEEFIAARSRHPMRVRMKT
                     GVKKDGTITANSMYALSDTGAYGCHALTVTGNTGHKAMALYVGDGEYRKAPNIRFYAD
                     VVYTNTPPAGAFRGYGVPQGYWPLDRHMEKIARAMGFDPIDFRLKNAIHPGEYHPFST
                     AWNEGREPRPEIIHTVGLEQCVAQGRAAINWDDKFGNQGWRSSVSGSKRKGIGVAMVM
                     QGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTGSDTVLAQMAAEVLGVPVEDIICY
                     SSDTDFTPFDKGAYASSTTYISGTAATKAAQIAAERIKIRAATMLGVEDHNAIQLRNR
                     HAHAPNGKSVSLAEIALDTLHKNNQEQIMGVASYVSPVSPPPFAAQFAEVTVDTETGA
                     VTVDRLVMAVDSGVIVNPLTASGQIEGGMMQALGYAVCEEMRYDEKGYAIERDFDRYH
                     LFRANEMPELETIFVETFEPSHPFGVKAVAEIPMDGVAPAVGNAILDAIGANVDDNPV
                     TPEKVWKALRAQTSAPS"
     gene            complement(42317..45229)
                     /locus_tag="PL000_03885"
     CDS             complement(42317..45229)
                     /locus_tag="PL000_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNC88064.1"
                     /translation="MSKAFTLIKEEQVPELNSIAKLYVHKRTGARLLSVINDDENKVF
                     SINFRTTPKDSTGVAHILEHSVLGGSEKYPVKEPFVELLKGSLATYINANTFPDKTCY
                     PIASPNLQDFYNLIDVYVDAVLHPLITEQTLMQEGWHFEINDPAEPLTYKGVVFNEMK
                     GAYSSPEGVLETRIIESLFPKHIYSVDSGGDPRNIPDLTYADFKAFWENYYHPSNSFI
                     FFYGNDDPDTRLKLMEGYLKSYKRKKVKSSVKPAKPFKRAKKVESSYDAGSDTDIEAK
                     NYLVVNWTLPETTDPVLNFSLRILGSILIGTPASPLKKALLDSGLGEDLAGLGLETEL
                     RHLIFSTGLKGTRARHARKIERLILDTLETLVRDGLDPDMITAAVNTAEFRLRENNTG
                     SFPRGIALMRRVLTTWIHDDEDPFKLLGFEATLNEIKARLASDPRYFERLIQTHLLEN
                     QHRTTLRLKPDPELARRLEEEESARLAKIRESLDAKQIAEMVENTRKLKLMQETPNAP
                     EAVATLPVLKLKDLDKENKPIPIEIVQLQDTQVLYHDLFTNGIVYLDLGFDLHAMPQE
                     YLPLTEIFARALLEMGTETEDYVKLSQRIGKSTGGIYGDALSTTAYGSRENVSKLFIR
                     GKATMSQAPEMLAILKDILLTTKFDNRERLKQIVLEEKSGVESGLVPGGHIFANRRVR
                     AQFGGSGWVNDQMSGIGYLFALRELAKDIDKKWPSVLKKLEAMRDALINSRSLICNVT
                     LDAANWAAFKPRLENFIFALPDKEVTLKPFDIKAHPEKEGLTIPAQVNYVGKAANLYD
                     LGYEFDGSADVVTGYLRMAYLWEKIRVLGGAYGGFCVFDENSGVFSFLSYRDPNLDTT
                     LENYDNAAGYLKNLDPNLFNDNELTKAIIAAIGDLDTYQLPDAKGYTSMMRHLTGKTD
                     AMLQKKRDEILSTNGEDFISFGEVLEKAAKSEAVAVIGSQAAIEGSKAGLKVTKVL"
     gene            complement(45278..46372)
                     /locus_tag="PL000_03890"
     CDS             complement(45278..46372)
                     /locus_tag="PL000_03890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNC88065.1"
                     /translation="MTRLPVNQILQGNCIEVLNALPENSVDLVFADPPYNLQLQQDLY
                     RPNLSKVDAVNNKWDKFSSFAAYDEFTSEWLGAARRVLKENGTIWVIGSYHNIYRVGA
                     IMQNLGFWILNDVIWLKSNPMPNFRGVRFTNAHETMIWAQKTKGAKYTFNHKAMKALN
                     EDLQMRSDWHIPLATGKERIKSNGTKAHPTQKPEALLYRVLLSSTKAGDLVLDPFFGS
                     GTTGAVAKKLGRDFIGIERDRKYIQVAQKRIAAVTKSPAEALTLPEKRNQARVPFGAL
                     LEAGFLSPGQKLFFKNDPSIRAVILSNGHLKYQEQVGSIHALAKALANGPVNGWDLWL
                     YKEHGKMKAIDELREKTRKLTGSKVGKLPG"
     assembly_gap    46485..46584
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            46663..47340
                     /locus_tag="PL000_03895"
     CDS             46663..47340
                     /locus_tag="PL000_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88066.1"
                     /translation="MNEKSSVSAPINRLSPSELTDKIAQANVFDLSALERNRMGRLSV
                     SQFFVSLAVVGNAVFNVAGFFMLNYFIPVGFMSLRSNSFLKAAACFGVSIAVIAIFLW
                     KGKKGASIGFDRYDIKDITQFPLKSLLIIDLLLWRVAEYRGSVSSDKATGIVRTKDYR
                     DPDNPTRVNPFSEEQTVSYYSYKAGRDKFPVSFEAYKALQGKLQDCRLYYLPLSKIMI
                     NMEVLQL"
     gene            47756..48007
                     /locus_tag="PL000_03900"
     CDS             47756..48007
                     /locus_tag="PL000_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88067.1"
                     /translation="MRFFIKSMEDLKKTGFKLEPTALLTTKKSLEIFIKSLELLVDDD
                     IPTHLAFASLSEALVWLDEVENTQAIQRIRDELLGKLRS"
     gene            complement(48086..48538)
                     /locus_tag="PL000_03905"
     CDS             complement(48086..48538)
                     /locus_tag="PL000_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC88068.1"
                     /translation="MMERIARCQTIVLQKGQILLICHANHQKGTIYWWLPGGGLEEGE
                     TFEQAAIRETREETGLLVRVERLLFEIEDRERTYHYQKYLTFLATPIGGELGLGFEGQ
                     AGGTNRLKEVRWFPLWDESKWESGFYEPHIHPLLVKAQQELISEKTKA"
     gene            complement(48540..>48740)
                     /locus_tag="PL000_03910"
     CDS             complement(48540..>48740)
                     /locus_tag="PL000_03910"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNC88069.1"
                     /translation="PDFVCIEKKLIIELDGSQHLEQQEYDEERTKYLNSISYKIIRFW
                     NSEVMKNMDGVIAAIIHALEDK"
BASE COUNT        11469 a        11663 c        12765 g        12743 t
ORIGIN      
        1 gctgtgttgg ataccgcacg gaataaaatt cttaagtaag catgctcttg ttgaggataa
       61 ttttattaag ccggttttcc ctttgaatcc gagacctatg gcatgttcgc tcatgtatcc
      121 atgccgaagc tgacaattgg caaaaaggtg tacgtcaagt tcgacccgaa caaacctgaa
      181 atttcggctc tgatgcgggc ggtagatccg taacttgctt gagtgatatg ggacaattcg
      241 ccccgaaata ccgtggcatg gaactgcctc agtttgagat aatctgttat aaaatacggg
      301 taaaattaca tatcattttc atcaggagtt gtaactgtgg attttagctg gatttcaaac
      361 cccgaatcgt ggattgcgtt ggtaacactt atcgccttgg agttggtact tggcgtggac
      421 aatgtgatct ttatttccat cctttcaggc aaattgcccc aggaagacca gcagcgggcc
      481 agaacaaccg gtatttcgct tgcggttatt accagagttt tgttattgct ctctctatcg
      541 tggattatag gtctgacaga gccgcttttc caccttccat tttttgaagg tgaagttgcc
      601 gaaatcaccg ggcgtgacct gattctcttg ttgggtggtc ttttcctcct ttggaagagc
      661 acccacgaaa ttcatgataa attggaaggg aaagagggat ccgcttctgc aaaggtggcg
      721 gcgaattttg tcagtgtgat cattcagatc atgcttttag atattgtctt ctcactcgat
      781 tcggttatta cggctgttgg tattgcagac aacattatta ttatgatcgt tgcagttgtt
      841 gccgctgcag ttgttatgat attcactgcc aagcccattg gggattttgt agaaagacat
      901 cctaccatca agatactggc actaagcttc cttcttatga tcggttttac cttgattgct
      961 gaaggatttg gtgttgaaat tcccaaggga tatgtctact ttgcaatggg cttttctgta
     1021 atggtggaga tgctcaacct gcgtgtacgg gactctcatg tcaagccggt caacctgcgt
     1081 gatccctata aagaggaaaa ggttgaattg cagcccgctt ttgttgctgc gtcaaaagct
     1141 gcaacagtaa caacagaaaa actaacaacc tcgcccgcta aaaaggcttc taccaaaaca
     1201 aaggcaaagc cgaaatcaaa aactaaaaag agctgagaac aaaagtggac ttctaaagaa
     1261 gtccactttt tgtttgatct atacccgatg ggcaccctgt tttccaggtt tcgttatgac
     1321 agatttggtt tcaatgcctc gctctacaaa aacactctca atttctgatc tggccaattc
     1381 cgcatgatca ggcggaacaa aggtgatgat cgagggccct gcaccggaga gtgccgctgc
     1441 accaaactgt ctggcttttt tgtatgctgc tgtccccccg gagatatgcc ttaaccgata
     1501 gggttgatgg atacggtcat ccattacttt ttgaagaagt gcaaggtttc ctgttcgtaa
     1561 tgcttcaact acaagcgatg tgcgcccgat gttgtacaca gcatcggcac gtgaaacgga
     1621 ttttggcagg accgcccgcg cggtttttgt cagccattcc acgatgggtt tgacaatgac
     1681 aatcgttaat tcaggcactt catatctgtg cgaaatgatt tcttctcctg ccattactga
     1741 aacgaccaat ccgccaaaca aggcaggggc aacattgtcg gggtggcctt ccatatcaga
     1801 tgcaaacttg agaagttcgt tctggctgag tggagacccc agcatttcat tcgcaccaaa
     1861 tagccccgaa acgatggctg ctgcactcga accaagcccg ctgctcatta agatttcgtt
     1921 gtgagcgcaa atgcgtacgg gactcacttt tttgccgcaa aattcataaa gattcgaata
     1981 cgatttggtc aagaggtttt ttgtgccttt attaagtttt tcggcaccct caccagtgac
     2041 gtgataagtc aacttttcgg caggctcaaa tgtgatttcg ttccaaagat ctaaagcaag
     2101 gcccagacaa tcaaagcctg gacccagatt ggcggaggtg gcggggactc ggatttttag
     2161 catggcgaca ttatataata cctgccgcca aaaattgctt ttttcgtaaa tttcagtgtc
     2221 gataggtata atcgtgtcat cacttaacct ggagttgcta tgacctatca aggcttgatc
     2281 catcgctatg ggcagtatct tgacttgccc gcccacacaa agacaatttc cctgctcgag
     2341 ggaaatacgc ccctgattcc catgccgcgt ttgggcaggg aacttggctg tgaattattc
     2401 atcaaattcg aaggcttgaa tcccacaggt tccttcaaag atcgtgggat gaccgctgcc
     2461 atcagcgagg ccgtaggccg tggtgcacag accgtgattt gtgcttctac aggcaataca
     2521 gcggcgtctg cggctgcata tgctgctcgg gctgggctcc gttccatcgt cctgattcct
     2581 gaaggcaagg tggctgttgg gaagttggct ggagccattg cctatggggc agaggtcatc
     2641 caaattcagg gctcttttga tgatgcacta acccttgtgg tggaaattac acaaaaaacg
     2701 cccattgctt tggtcaattc gatcaaccca tttcgcattg aagggcagaa aactgcgtct
     2761 tttgaaattt gtgacgatct tggttcggcg ccggattggc tatgcctgcc tgttggcaat
     2821 gcggggaata tcacttcgta ttgggcgggt tttaaacaat ataaaaaagg gttgccccaa
     2881 gtgctgggag tgcaggcagc aggggccgct ccacttgtgt taggtcatcc cattgaaaaa
     2941 ccagagacaa tcgctaccgc cattcgcata ggcaagcccg cgcgcggcga gcaagcttta
     3001 caggcggcgg aagaatccaa tggaaggatc attgctgttt cagacgaaca gattctttcc
     3061 acacagaaga tattggccgc ggaaggggtt tgggttgagc ccgcatctgc tgcggggctg
     3121 gctggtttgt tggcagagtc agcttccggt aagttcgaag cgaaaggcaa acgaatcgta
     3181 gtggtttgca caggccatgg gatgaaggat ccatccatca tcaccgaatc tttcaaatcc
     3241 cccaaagtga tctcggcaga ttacgatgcg ttgatcagtg ctgtgggtgc gtagtactgg
     3301 tgtaccattc gtatggcggc gggcctttat ataataaggc ggatttaccg atcaatccaa
     3361 atttgctaaa aggagccaaa catgtttttt aatgagtgga atattcttgt tgttgatgat
     3421 gaacccgacg tccttgctgt gaccaaactg gctttgcgcg atgtgaaggt atatggtttg
     3481 cctgtaaaga ttcatacggc atccagcaag gccgaagcga ttgaaatgct caagggtccg
     3541 ctggctttac agggctctac agagccgatt gtggctgtgg ctttggtgga tgtggtaatg
     3601 gaaaacgatc aggctggtct cgaactgtgc aaattcatcc gtgaagaggc gaatggacat
     3661 tcagtgcagt tgttcatccg tactgggcag cctggtgttg ctcctgaacg ttcggttatt
     3721 gataactatg atatttcagg ctacttcacc aaagttgaat tgagcgagca gaagttgtac
     3781 acgttggtga agagcggtgt gcgtcagtgg tttaccagtt ggtatgcgtt gatgactgag
     3841 gaatcgacaa ataacattgt tacgcactct ggctctcgca aagacttttt ggagaatggc
     3901 attggttggt ttggtgagcc tggtccgcag gaaggcgagg gtgtcactgg ttttattttt
     3961 gatagaaacc ttattgtgag tgactatccc acccagatca agccgttgta tgatcgtttg
     4021 aatgcattga agcccgtcat tgaaacggca gagggccaca agctcgccat ggatgaaatg
     4081 ggcaatcttc tggtcaagac ggtcaagacc aagacgacag cagattatgc ctatgtaggt
     4141 gagtcgtcca tggtcatgcc gcgattgctg cttgaattga ccttcaagag tggtttggtc
     4201 ttttccactc tatggaagaa gatcaccgat gacgaaaaga gagccgctgc taaaaagcct
     4261 gttgcgaaaa aatccgctaa aaaacctgcc aagcctgcca aaaaggctgt aaagaaggtc
     4321 gcaaaaaagg ttgcagccaa gcctgctaaa aagacgacga agaaagccgc aaagaagaag
     4381 aaatagtggt tttcttgttg aaacgttctt gaaaatgtaa cccaaggcgg atgctgtgct
     4441 gagtaattga taagccatgc atccgcttgt gttttgaagg tccatgcaaa atgacgaaaa
     4501 agaatcctgt gctcaatcca aatattctgc aaaagttttc gttctttgct tctttttcca
     4561 aagaccaatt ggaagagatc gcaaagtcgg cgccaaaggt ggcctgtaaa actggcgaga
     4621 tcgtatttcg gcagggtgac cgctctgcga cgatgtattt gattctcaaa ggtgcagtga
     4681 aaattgagag agaagacagc gtgggtggga tcgttagtgt gggccagtta tctgtgcatc
     4741 aggtatttgg cgagctggcg atgttgagca aggagcctcg tcaggcgact gttaccgtga
     4801 cgaaaaacgc cgaattcctt gtgattgacc gtaaaatgat gttggagatc attgccaaat
     4861 cagaccccga ggagatcatt gaagtttttt ctgtattgag tgagcagact cgtgctgcaa
     4921 acgaccgcga atttcatgag accctctcgc ggcgtaccct tgaagcacag atggaagtgg
     4981 aaaaacaacg cgctctgacg cagctggtgg ctggtgtggc gcacgagatc aatacacccc
     5041 ttggaataat taacacagcg gtcagtatta tggctcgtga attatcagag cccagcgaaa
     5101 tcactgccca gcgtgcggca gatattgccg aatcgctcga attgatgcgc cgcaacgtgg
     5161 agcgggctca tcgtttggta caggatttca aaaaggtctc cgccagtcag ttaatggacg
     5221 agattgaaac ctttgacatt tctgaaacca ttcgtgaaac tgttgacttg atgatggttg
     5281 gcttgaagag tagtcaaatt caggccaaat tccacgataa attgacactc acgcaaaaag
     5341 catggatggg gtatcgaggc gggctatctc agattctgat caatctgttg accaatgccg
     5401 aacgacatgc ctatcccaat ggagcgggcg gattgatcga cattaccatc gaaatgttcg
     5461 acgaaaagaa ttatcgcctg accgttcaag attatgggca gggtattcca gctgaaaacc
     5521 aacctcgtat tttcgaaccc tttttcacca cagcacgtgc tcgtggcggc actgggttgg
     5581 gattggccat tatccataac cttgttgtca acgttttgaa gggtgaaatt caattaaggt
     5641 cagaaataca gaagggcaca gcctttttgg tggtcttccc aaggaatttg cccaaataaa
     5701 ctcttttcag cctgttcaag ggattcaatc tcctacttga cattcccatt taatgtcttg
     5761 ataataggat ggatgcaatt gaattcatta aaaaccgaga gtgctgaaac ctgtccatga
     5821 acgaaactcc attggttgtt gaaaacttaa cgtttaagta ccgtgttcgc cctgagcttg
     5881 cgatcgaaaa catttcgttc gagctaaaac agggtgagtt gcttttaatt gcaggttcaa
     5941 gcggctgtgg caaaaccaca ctggcccgtt gtattaatgg gcttgttcca cgcagctacc
     6001 gtggcgagcg cagtggacga gtccttttgc atggcaagga agtgaccgaa ctaaagatcg
     6061 ccgaaatggc gcaggtggtt ggcaccctgt tgcaagaccc tgagcgtcag atcgtagcca
     6121 gtaatgttta taacgagatc gcttttggcc ccgaaaacct tggcctgccg catgatgaga
     6181 ttgaaacacg cattgaacag gccctgaagc gtttgaagat cgaatatttg cgcgaccgtg
     6241 agaccttcaa tctttctggg ggcgaaaagc aaaaagtggc actggctggt ttgcttgcaa
     6301 tgaacccttc catacttctg ctggatgaac cccttgcttc cctggacccc gcctcagcac
     6361 acgatgccct caagatattc agaagcctcg cagatgaggg aaaaacaatc attctggtgg
     6421 aacatcgtgt ggaagattcc atcgaagcga aacccgaccg cctgctttac atggagagcg
     6481 gtcgtatcaa gtatttgggt ggcattacca gcctgccaaa agaaattgac catacccagg
     6541 taaaactccc cgctcaatgg gtggtgcaac gggtcaagga agataaaaaa catgccgatg
     6601 ttccgcccgt tcaaaaaggg gaaagtggtg atgagccgct ggttgttttt gaagatgtca
     6661 gttttcgata cgacgatgaa tacccgctca tcttgaaaga tgtcaacctc accatcaatc
     6721 gcggtgacct gatcgctgtc cttgggccaa acggcgctgg aaaatccacg ttggtgaagc
     6781 atgccattgg gttgttaaaa cccacctcag gccgtgtgtt ggtgcagggt gaagataccc
     6841 gaaaaatgag cgtcgcccaa attgcgcgcg tgttggggtt tgtctttcaa agtcccactc
     6901 atatgttgta cgctcccacg gttcaagagg agttggaatt cgggcctaag aatctcgctt
     6961 ttgacaaaga ggcaatggac aaatcggtaa ctgaaagcct caacgcggtc aacttgaaag
     7021 gttttgaaga atacccgcct ttggggctgt cctttggtca gcaaaagcgc acaactattg
     7081 ccgccgtgct tgccatgcaa tcgaagatca tggtaatgga tgagcctact gccggacagg
     7141 attttgccaa ttacacgcgt tttatggatg cattgtgcag cgcctccaat aacgcaaaat
     7201 cgatcctctc tgccaatttt gactcaaccc tcttcattac acatgatgtt gatctggcag
     7261 tgacctatgc aaaccgtgtg ctgctttttg gagacaagca cgttgttgca gatggaaaac
     7321 cagaggaggt cttgaaagat attgacctgt tgatgaagta tcgtgtgcgc cccacttcgc
     7381 ttcttcacct gaacctggac ctgtttgcaa aaacgaagaa gtttttaccc gctgaagcat
     7441 tggccgggtt tgtgtcatga tcttccctga aacgcattga cggattcagg gtttatctag
     7501 gtatagggtc agctcgtatc gaataccaat caaaaggaga gaatcatgaa cgacaaaagt
     7561 acttgggaat ttggaacccg ccaggttgta tatggtgcaa tcggcgcggc attgtatggt
     7621 gtgctgtcac tggccaccaa ttacttcccc cttccggcgg ctggtaatgt aaccttccgc
     7681 ccggcggtcg ccgttttgat cttttttggc gcggcgtatg gcccctgggt tgggttgctt
     7741 gcggggttca tcggcaatac ccttggtgat gctgccatgg ggtggggctt ctattggaac
     7801 tggagcctcg gaaatggtct gatgggtttg gtcgctggct tggcgatgcc aatgctcaaa
     7861 gatttcaagg ctcgtggcga tattatcaag gccattatct acggtttgat cggtattgcc
     7921 gtgggtatgt tgtttgcttc agtcactgaa aaattcattg gcggccttga ttggaataca
     7981 gccctggttg gatacttcac accggccttt attgggaatg ccgtagtcgt ggtgatcctg
     8041 ctgcctgtgt tgatgatcgc tttcgccgca gtcgcttctc gtagaggccg ctaaaacata
     8101 tgttggtcac ctggaagtac cgtccccgcg atacatttat tcagcgattg gatccgcgca
     8161 caagatttat ttacatggtg tgcattattt tcgcgctgac cctgcccgat atctgggact
     8221 atcgcatcat ctttcctctg ttcattagct cgctgaccct gtatttgctt gctcgaatcg
     8281 agtggaagga tgtccgacgt gcctggatat tcatcggtgt cttcgttttc tttatcgtcg
     8341 gcttgaacgc cctgctatcc ggccgaggcg gtcctgacac ggtgaccaac gtttattctc
     8401 caaccatatt caacctgcct ctgaaaattc cctttaccaa tatcggctgg gatatcccag
     8461 ttacagttgc aaaattatgg tttgccgtca accagatcac gcgtatgttg accatggcca
     8521 ttctggcgtt tcccattccc tacacggtca gccccactat ttatgggacc acttttaaga
     8581 ggatgggtac ctcggataag ttttccttca cgatggacct ggcttttcgt tttctgccga
     8641 cctttgcccg tgatttgatg atcacaatgg atgcccaacg ggcgcgtgga tacgaattag
     8701 acaagttgaa gggcggcata gcggcccgtc ttcgaaaact tgcaccattg atcattcccg
     8761 tggtgatgca atctacggtg acaggggaag aggtgattga cgccatggat ttgcgggctt
     8821 ttggtacggc tccccgcaca tggctgcaga gtcaagactt gcaatacaaa cccatggatt
     8881 atgtgatgct cagtttgggt ttggcgattc ttgtagtgtg ttgtattttg aaatgggggt
     8941 ttggcatagg cggtttcttt gtgccagatt ttttcatgaa cctttttgtg taaaacaaaa
     9001 acgcatcgcc aaaagcggtg cgttttttat aaggcttttt gacacattcc tagttttcgt
     9061 ggcgtggata ggtttctccg gccaatactt tgaaatcgag atggttttgc tcaggggcga
     9121 aaacgcaggt ggcaaaatct gtaaatgcgc agggcggaga gtaggctcgg ttgaaatcaa
     9181 tgaaaatttt tccgtcggct tcgatatctg cgatcaagta gcggcctgtg ggataggtct
     9241 catctttgct ggtggggtcc cagaagcgga caaaaatcgt tccgtcgtct tccttgttga
     9301 tatccagttg gtagtgattt ccaccgaatt cgaaagataa ataaccttcc accgggaatt
     9361 cggatttttc gcctgttata tctggaaagt aagccatctt ggggcgatca tacggcgtaa
     9421 atgtggcagg gatacgatat tgctcatcga tgtcgtacca gcttcgggcg gggaaagatc
     9481 gtctctcttc gcgttggtta tcccacatgc gtacgcccag tctgttgccg cgttgaatca
     9541 ccacgaggca gatatcctca aatttaataa agcttgggtt ttcagaaata tcggggtgaa
     9601 gaatggcaaa atctgaaagt ttttcgttta catgcacttt atgaccatcg gtggtgcgca
     9661 tgctcactga ttttccgttg tattcaagat accctacaac aggtgaaaca cgggcgggca
     9721 attgaatttc gcacttgggg tcggcgccaa actggttttt gccgagtttg agccagtaga
     9781 gcccggcaag cgagagccat ccattttctc tgcggatggt ttcatcgcgt tccttgcgcc
     9841 attcattgag cagttgccga taagtttttg tggtcatgtt tgggattata ccaacaaaaa
     9901 aaggcgggcc gttgccgacc cgcctttctg atttgttatg ctgttctacc caactgtggg
     9961 gaccatggtg aggtagataa agaggccagc aatgaatgca agcaggccga acagtgagaa
    10021 gagggtgcgc atggtgcttt ctgcgccaag cagggacgcc caagccgcga gtgcaagacc
    10081 aagggccatg atcaacatga cgagttgtag ttgatcgccg cgctcatccc attgtgacgc
    10141 aaggtcaaag ttggtgtcgg attcatccca gagaacggtt gcttcggtgt acatatctgt
    10201 gtaatattgc tcatcccaga cggtttcagg gtcacgttcg atggctgcgg caagttgctc
    10261 tgagaaactg gtttggtagt attctgcgat ttcggggcgt tctccggcgt tgatatacca
    10321 gttgtcgaag tagttgtagt cctgggtgat gtcctggttg gcgcgcaagt actcggcgtt
    10381 gccgtcattc agggtttgca tgccaaggat ctcatacttg ttctgctctg aatcagacat
    10441 ggcgccctga tagctggcga tggcggtaaa tgcgcttagt aaagcaatca tcgtaccgag
    10501 gaagatttca ttgctcaaaa aaccaaaagc ctttttcatt tctctcactc cttatgaatt
    10561 ttggataaat tacttggtgg aactacggca gagattataa aacatgattt tgcattgcat
    10621 atttacaaaa ttgttataaa atcatgccag tttgcaagaa agtatattca tgcctttgtc
    10681 aaaagactga cagggcccca aggagaatcg gtatgggttt gaaaccagat aagtggattc
    10741 gcaagatggc gcttgaacag aaaatgattg aaccctttgt agacagtcag gtgcgtgaag
    10801 gggtgatttc ctacggtgta tcctcgtatg ggtacgatgt tcgagtggcg aacgagttca
    10861 agatttttac gaacgttttt tcagccacgg tcgatccgaa gcaatttgac acaaaatcaa
    10921 tggtggattt tgtgggtgac gtttgtatcg tgccgccaaa ttcttttgcg ctggcgcgca
    10981 cggttgagta tttccgcatt ccgcgcaaag tgctgactgt gtgtctgggc aagtccacat
    11041 atgcccgttg tggcattatt gtcaatgtga caccgtttga gccggagtgg gaagggtatg
    11101 tgacgctgga aatttcgaat accacaccgt tgcctgccaa gatttacgca aacgaagggt
    11161 tggcgcaggt cttgttcttc gaagctgatg aagaatgtga cgtttcctac gccgataaaa
    11221 aaggaaagta ccagaaacag caatcgattg tgttacccaa attatagtat tgtgataatc
    11281 tgatactgat tgaaattggt tttatcaagg agagcggtta tggctgattc caatgaagca
    11341 cagacaatgc aaaagcaaat ggatgacatg aacaagatgt tgttgcgtgt catctcgcat
    11401 ctggcacggt ccacagatgg tcagacttcg ttgattctga gggaccttac acagatttcc
    11461 agcaccatgg ccgcactcaa gcaaagtatc actggctatt tgagcacgta tgaacgtcag
    11521 gtgggggcgt tggtgggcgt aggaagcgca attaattctt cattggggct gaagcgagtg
    11581 ctggaaatgg tgatggattc gctgatcgcc ttgatgggag cagagcgcgg tttgttgatg
    11641 ctgcgcgagc cgaatggcga attgaaggtt cgtgtagccc gtggtggtga ccgtgaagac
    11701 ctggcacagg aagttttttc gatcagcaat acagttgtgc agcaagtggc tgagaccggg
    11761 aatgcaattt tgacaaccaa tgcgcaggat gatccgcgtt tcggcaggca ggtcagtgtg
    11821 gcggcgtatc gtttgcggtc aattttatgt gcgccgctca agatcaaaga taacttgatc
    11881 ggtgtgatct atgtggataa ccgtgctcat accggtattt ttggcaacaa cgatctctct
    11941 ttgatttcag cgttttccaa tcaggctgct gtggccattg ataatgccag cctcttcgat
    12001 gatctacagg aaagcaatgc tgaattggag atcgcgtatc aggccacact ggaaggttgg
    12061 gtgcgcgcac tggacttgcg tgacaaggag acagaaggcc atacgcaaag ggtgactgcg
    12121 ctcacacagc gcctcgctca agccatgggt gtggatgatg atgccttgct ccacatcaca
    12181 cgtggagcgc ttttgcacga tatcggtaaa atggcaatcc cagatggcat tctgctcaaa
    12241 cctggcagtc ttaccgatga ggaacgggcg catattcaga aacaccctgt atatgcctat
    12301 gagatgttaa gcccgatcaa gttcctacac cctgcattgg acattcccta ctgtcatcat
    12361 gaaaaatggg atggtacagg gtacccgcgc aaactaaaag gagaggagat tccatttgca
    12421 gcgcggatct tctcggttgt ggatgtgtgg gatgcgctta tttcggatcg cccataccgc
    12481 aaggggttgc cgccaagtga gattcgtgaa cgcattcaaa aagattcggg ttctcatttt
    12541 gaccccaagg tggtagagac tttcctaagc cttgataatc ttgctgtctg aatttctctt
    12601 gtgaatccag caaccataaa gcacaacgac atggaattag tttttctcca tgtcgttgtg
    12661 cttttaagat cttttattgt agtttgctgt tgtgaactgt tacgtcccga gccattgacc
    12721 tgccatcttg gccaaccctg cggcagctcc ctgtcttgca gtgcattgcg gcagggattc
    12781 gaggaacaaa cgcccgtatt gttttgtcag tacgcggcgg tctaaaatgg cgacgatgcc
    12841 gcgatcagag gcagaacgga tgaggcggcc gaatccctgg cggaatttca gaatggattc
    12901 gggcaggtag tattcgttga acgaatcttc ataaagttct gagcgtgcag caatgatggg
    12961 atcagagggg acatcaaatg ggagttttgt gatgaaaacg accgaaagcg catcaccggg
    13021 cacatccaca ccttcccaga atgagcgcgt gccgagcaac actgcgcggt cggtggattt
    13081 gaacgattct agcagggcat tgggtgaagc gccttcgcct tgctcaaaaa caaaaatatc
    13141 ctcgcgtgcc aatggaccgg tgatggcctg tgctgttttc ttcagcgcgg cataggatgt
    13201 gaacaatacg agcatccgcc cgcctgtggc tttggctgtg gcgatgatgg ttttatcgag
    13261 catttgctga tagccatgga tatttggttc agggatgtcg tttgccacgt aaagcagtgt
    13321 gctggattcg taatcgaagg gcgagccgag cgcaaccgta tccacttctt cagcggagag
    13381 ggtgttgcgg atgtattgaa actctccatg tgtggtgagt gtggcggaag ccatgatgac
    13441 actggctttt tcatgccaca ggtgtttttg catcagtgga ccaacccgca gtggagccgc
    13501 gttgagcgaa agcctttcgc cgcgcggatt gacctccacc cagtagatca gttcattgga
    13561 tggtttgtgc atcatgcctg aggatgcggc ttccgcctcg gtcatccgac gcatgagact
    13621 gctcaagctt cccatgatat cttccacgct gtcatggcct tccgacatca gatcgccgag
    13681 gcctttgtac agttcatcga gggttttcaa taacagtgac atggtttcgc ccacctgccc
    13741 ccagcccatc tctacatcgt cccaaccggg cagggtgcgt gtggcaggca tgacgcgcat
    13801 ctgccatgaa taattgcttt gcggctgtcc ttcacgctga atggcaatga aatcgctcag
    13861 gttcataaaa aactgtttag agagttgctc aatgcgaaat gcctggtcgg tggcgcgttt
    13921 aattttttgt tgaagcaggc caaagtcgga ggggcgaagc gaggcatgca gatcggtcaa
    13981 aatacggccg agaattcctg ccgaagatcc gcccaattcc ttcatcatgc gttcgaggtc
    14041 gttttgagtc attcggaaag ataatgcgcc tgttactgcc gattccatgt ggtgcgcttc
    14101 atctatgatg acgtaatcat attcgggcaa tactttgctt ccagatgcca cgtctgacag
    14161 cagaagcgca tggttgacga tcaagatatg agaattgagc gcggcttgtt tggcacgctg
    14221 gaatggacag gatcccccca tgcgccccat gcaggtttcg gtggtgcagt tatcgtcctc
    14281 tgcagaaagc cgtgcccatg cttcgcgttc agtggggccc gtgaggttga gttcgtttcg
    14341 gtcgccgctg gcgttatcga gcatccacac catgattttg gcgagcaccc gcatttcatt
    14401 ggcatccttc ggcccgtgcg agcgcatgta ttcgagcttg cgcggacaga gataattgcc
    14461 gcggcccttc atcaccgtgg cgttcaggtt ctcaaggttg agcgcggctt tcaagttggg
    14521 gatatctttt ttgatcaact gatcttgcag gttgatggtg ttggtggaga caaccacgcg
    14581 ggtgctgttt tgaaccgcga aataagccgc aggtacaaga tacgcgaagg atttacccac
    14641 accggttccc gcctcgacca tgagatggtt gcccgtggaa agggcattgg tcacagcctt
    14701 gagcatttcc acctgttcag gacgatgctc gtatgattcg aagtattgcg agaagggccc
    14761 gccatattca aggacggcag cgatctcttc agggtcaagc ggaagcggct cttcggtctt
    14821 ttgaatggag gctgattctc gttttgctga atcaaacatc ggtccagaaa aagaggcgag
    14881 cctggctcgt ttggctttgg gtgcttcctt ggcgcgttgt ttcaatgcct gttggaagac
    14941 ccagcccgca tcccaatcca caaaatcgcc gagctgcacg atctcattca aggtttcgag
    15001 cggaagttca ctagccattt caaacaaacg gacataagcc gcctgcgtga cgcgggcatc
    15061 atccaatgcg cggtgagtgg cagggagcaa aatattgagt tgctggccca gcgagccgag
    15121 gttgtaacgg ctggcggtgg gcatcaacac cgaggcgagt tcgtaggtat ctatggtttg
    15181 ctgatattcg aataaccccg ccttgcgaag aaaaccgata tcgaatttca cattgtgacc
    15241 caaaatgggt gcgtcaccaa caaaggcgag taggtcgttg gcgatgtcgc gtaaacgcgg
    15301 tgcctgccgt accatggcat cgtcaatgcc tgtcagcccg gtaatgaaat caggaatgtg
    15361 cctgccgggg ttgatcagcg tgttgaattc actttcaatg cggcggccat tgaactttac
    15421 ggcggcaatt tcaataatgg cctcgcggtt ttcgtcgagg ccagtggttt cgatatccag
    15481 agagacaatt gaggtcatat tagaacaagt gtaccatgaa aagtttgttt cgcttgccgt
    15541 gcgggtttat gagagtggct gacaagaata gtttgtgact tcaccatctt tttgccgctc
    15601 ccgcattcat cccttttcag atttcaaagg gcggttggct tgatcgaaga ggatggctaa
    15661 aaatatccac agcattaatc ggaagatttc cactgccagc atggtatggc gtgtgacgcc
    15721 aatggagtcg gcaaaaaaat taataaccat cattgccgat gcgctggcga tcagccaaag
    15781 actcaaccag gcccaggcgc cgaattcccc atttcttcgg gcgatcgctg aaacggccaa
    15841 tcccatcaat aacagcagat ccaggataaa aacgagatgg gtgtttggat gcagaatatc
    15901 gttgagcgca ttcaaaacct ttcttgtgcc tgtttgctcc gagaagaaat agggttgtgt
    15961 gttttcagag aaaatgcccc ccaaattcga gatgaatgag gtaagtgtgt agccagggtg
    16021 agcaagcagg aagcgggcat aggcccgtgg tgcgttttct ctgaaccagg ttgggtattc
    16081 agcagatttg gggtctggca tgccgatgcc cgtcataaac tcagcgcggg ctgggtatgg
    16141 cagaatgaac tctgcaaaca cattggtgag cggttttttc cagcgcctgc tttccatggc
    16201 gctttgcaac cctacaactg tcagaacaag aatgaccgca ctgatgacga gtacgctttt
    16261 gtttttgcgc aggcgcggaa aaatcaccag tgacacggcc aaaaacgcga aaagcacagc
    16321 aagggcatac acgttcgaat ctcttgtaaa agtccacagg gccagtgaaa ccagggtcag
    16381 gatggttagc agaattgagg tcggcctttg atcttcttca atggcagtga agcaaatttt
    16441 cagcaacaag gccaggctga tggcaaacag cgaaaaggtc aatgactcag agccgaggat
    16501 gctgtcccag tctgcgatgg caggcgtaaa accaaatgcg gtgatggaga ttgccgcgaa
    16561 cagcttttca aatccgcctg tcagtttgct ggctacggtc aatgccagca ggctccaggc
    16621 aaggatggaa agcaggttct gcaacagggc aatccttgta aagcacggct gtaccgcccg
    16681 ctcggtttca agcccaaggg cggggtggct gatggcggtt aattcgcact cctgaacctt
    16741 tgccagatga taaacaaggt ttgtggtgaa aagacggctt ctggtgagca tatcccttga
    16801 aaagagggga ggtgcggcct cgtcgatata actgggcgta tccgcattgg ccacagaaag
    16861 attgatactt ccgtacgcct tcagccgaaa gaaagccatg cctgccagaa tgacccagat
    16921 gagaagggtc accccgattt tttcagatac cttcatggaa tcctctgtag caggatgtga
    16981 ctaggattcc agcgcgtcac tcagcgcctt gatctgatcg ttggaaagtg accgattctt
    17041 gccgccctga cgcatgaatt ccttaagctg tttcaaacgc atggccgcga tgggagaatc
    17101 gcccgcatcc acttcgatct catcattggt gaagaccaaa atgggtttga tctcagggat
    17161 ggtggattcg tccatttttt ttgcgagaaa ttttctcaac gcctgcactt cattttcagc
    17221 atctatttca gggcgaccca aaccctcctg cccaaagacg ctcatgtagg cctgtagaaa
    17281 ccctccattg ctcagtttcc agcgattctt ttggtaagag atcttccccc gctggtgcag
    17341 cggatgcaac acccatgccc ctgaaggccc aatcagcagg tgtgaaacag gcgatgaaaa
    17401 atgatagatc acaaaatcgc tgtgcaagcc ttttagccct gcgtcaaact tctcatccgc
    17461 gcgtggcgac ctgccccagc ggttgcccat gtaaatgctg atctgggtca tcgtaaaccc
    17521 gatcacaagg cagacgatcg aatatataaa atattctggt acggtgaaag aaatgtacaa
    17581 gcccagccca agcactatta gagaggcaag gctcatccat tggccgattt ttccattgcg
    17641 ttcgatcaat ttctcgtttt tgatgatttt catttagctt ttcaaactct cttctatggc
    17701 agatttcatt gcgtccagca cttttacatc cacggcatgt ttagccacgc ggacagcatt
    17761 cttaatggcg atggcatccg aacggccatg accgatgaac accagcccgt tgacgcccaa
    17821 taaaggcgcc gctccctgct cactgggatc caacagtttc ttgatggctc ccaaagcagg
    17881 ccgaacaagc attcctccga tcttcgtcaa aatgcttcca ttcttgatga tctctcggat
    17941 tttatcggtg atcaacttgg cgacagcctc cgaggatttc aacatcacgt tccctgtaaa
    18001 accgtcagtc acggccacat ccactttgcc gccgatcact tccttgcctt ccacatttcc
    18061 aaaataattt aattttgaag ccttgaataa aggcgtggct gcctttacca attcattgcc
    18121 cttgccttct tcttcgccat ttgaaaccag gcccaccttc gggcttttta caccacgcac
    18181 cttctcggcg taaatacttc caaggattcc aaattgatag agattctcag ctttgcaatc
    18241 ggggttcgcg ccgatatcaa gcacaacaca cgtgcctgtt gctgttggaa agatgggggc
    18301 cagggcaggg cggtctacac cacggatgcg ccccaagcga aatagcgcgg tcaccatacc
    18361 cgctccagtg ttgcctgcag ttacaaatgc gtcggcctcg ccattcttca cgaggtcaat
    18421 gcccactgcc atcgaagtct tcgcgtcctt tgcgcgtgcc ttcaatacca aattttcacc
    18481 tttgtcttcc atcgtcaaca tttcgggtgt gttgacgatg cgtatcttca acccttctgt
    18541 attttgtgtg gccaatacag gtttgatcct ggcttcatcg cccactaaaa tgatctccgc
    18601 cccatattca cgtgcggcca ttaccgcccc ttccacgtct ggaacagggt aactgtcact
    18661 tcccattgcg tctacaacga ttcgagccat agaaatctcc tgtgataatt atgcttgaca
    18721 agaaaagcaa gccgattata atacgccctg cttacgcgct ggtgctggaa ctggcagaca
    18781 ggcatggttg agggccatgt gtcgcaagac gtggaggttc aagtcctctc cagcgcacac
    18841 ctcgtcccca ttatcaaaat ggggacgttt tgtttacaaa aggggcttat ctggcagggt
    18901 aaagtaaaaa ctcgctccat ttccagtttc cgactcaacc caaaccctgc cctcatgaac
    18961 ctcgataatg cgtttgacga tccccagccc aattcccaac ccatcggctt ttgcatttaa
    19021 tttgttgaac aacccaaaga tctgttcatg atattttggg tcaatcccca tcccgttatc
    19081 ccgcacaaaa aagacacgct cgttattcct cacctccgtg tcaatctcga tgactggcac
    19141 aggctgttcc cccatgaatt tggcggcgtt tgtcaccaga ttttggatga cttgaacgat
    19201 tcgtgcaaaa tcacaataca cgatggggaa ttcgcttccc accttaaccc tgacctgttt
    19261 ttcctgtaat aggccactcg atgccttgag cgcttctcct acgatctcgg caaagtccac
    19321 ctcccgcggt ggattcatga tgcgcccaat tcgcgataag tccagaattt catccaccag
    19381 tttccccatg tttttcgccg cttcattcac ctgactgatg tctttatgaa ccctgtccat
    19441 attccctgcc aaggcatccc tctccagata tcccaaatat cctgtgatcg taataagcgg
    19501 cgtctttagg tcatgcgata cagtgatcgc aaagcggtcc aattcctggt ttttcagttt
    19561 caactgacgg acaagttctt cacgttccat ttccgctttt tctctttcag cgatttcctc
    19621 cctggctcga attaaactgc gcctgatgtt ttccactaaa agatgcactg cattgctggt
    19681 aaccaggatg atcgcgctgg caaaaagaaa atcctcgggc aggctggcca attctgtgtc
    19741 ggctttgtaa agtcccagca tatcgccaaa gacaagccac ccgaggcata aaatgattcc
    19801 tcctgtcagg tagggcagca ctctgccgcg cagaatcaac cccgtcacaa tcagaatgat
    19861 cggaaaagcc atattcgcca cgtcatggat cccgttgcca actgtggaga tgtaagtgag
    19921 cagtattatg gttccagtgg caaaaataga gatcggcaga gaaagctttc cgctcttgat
    19981 catcagcagg gatatgatga tgggcagggt gcccgccaga attaccaggg tttggatgta
    20041 cctgcccttg agtaactggt taataaccaa taccagcaga atgaaaaggg aggaataggc
    20101 cacccatcgc accgtttcag cgatttgctc ttcattgccg tagacgcgtc ttctcaattg
    20161 cgaggaaagc ggttccttgt agccagattt atcgtccata tcacacctgt tgtgttacgt
    20221 gcgtgccgtc aatgtaaagc agaaggtcgt gccttttcca gactctgatt caacccaaac
    20281 cctgccccca tgaaattcaa tgatgcgttt tacgagtgca agcccgatcc ctgttccatc
    20341 agtcccagca tccagtttgt tgaaaaggcc aaagatgcgt tcgtgataga gcgggtcaat
    20401 gccaatgcca ttgtctttta cgaaaaatat ctgctcgttc tcttgttgaa aagaaccaac
    20461 cttgacctcg gggtttgagc ggtttcccat gaacttgatg gcattgtcga tcaggttttg
    20521 gtacacctcg accaggcgat ggcggtccac ttccatgctg ggaagatctt tctcaataat
    20581 gaccttgacc ttgttgctgg tcacttgttc gtgaagcagc cttgaggctt ctctggcaat
    20641 atcaccgatg ttcgcgtttt caggcttgtt aacgaatcgg ccgatccttg aaagttcaag
    20701 cagctcgttg agcagacgct gcattttgat gactgcatcc attacgcgct gaatatcttt
    20761 ttgggccttg gcagtgtttc cattcaggat gtctttttcg agatagccta caaacccgcc
    20821 aatggtgatc aacggggctt tcaggtcgtg tgagacagtg taggtgaagc tttcaagctc
    20881 attattttta ttttgtaact cgatcaataa ttcatctatt tttgtgatgt cgtagagcgt
    20941 gtagagtaca catgcttcat tattgactgt aatcggtgca accgataata aggctacccc
    21001 tgcttcgccc gatttgcgcc tgtatctcgc cttgaattca cgaatgaagc cctgctcttg
    21061 aaggagttgg tggtgtttgt tgtgttcgtc agggtcagcc cagaggttta ggtcgcttgc
    21121 tttgtggttt atggcttctt cgcggctaaa gccgatttcc tgactgaacg cctcgttgat
    21181 atccacaatt gacttgtcgg aaaggcggcc gatcaccatg ggtacaaatg tgtggtgaaa
    21241 ggttgtttgg aatttttctt ctgatgcgcg caatgcggtg tctattttct ggcgtttaat
    21301 ggctgacccc aacatgctgg cggtaacctg catggcatgt acttcggcct ttgtccacgc
    21361 catctcacgg atgcaatcat cgaatccgat caccccccac catttgccat ctacaaaaat
    21421 gggtacatcc aaaagcgttt ttatatcctg gcggctggga gtgtcctgcc atacttcttc
    21481 aaatatcttt gtggtgttgt agaaaggttt gccctgactc accacggaat accaggctgc
    21541 cagtccttct tcaaggacgg ggacgttttg aaagtcggga ttatcaagct cggaggtttg
    21601 tcctgggcca gtccattcgt atttttggct ggttaccaga ttgccgtttt cgtcgaggtg
    21661 gttttcgaaa aggtaggcat ggctggcgtg tgtagcctct ccaagcaatt tgaggaggtc
    21721 attgatctcg gttttccaat caggggcttc cagcagcctt acagcagact gggcaatcgc
    21781 atcaaggagt acatcgcttc gccttggaag tttttcctcg ggggcatttt gcttttcatc
    21841 tgatgcggat tttgtctttt tcatggatat tcaagccgct tttttgaatt gggataaaaa
    21901 aatctctacc actaaatgat agagatttta gcagataaac ttgaccttac acgatcaaac
    21961 tgcaacaggt gacggcccct tccgcttttg ccagttcagc cacgttgaca gcctgaattt
    22021 tgtatcccct tgcttcaagt ttgttacttg tttttggaaa ggctgtaggg aagataattc
    22081 catcgccaac aggcaggcag tttgccgcaa acggttctgc ggaatccacc tcgatccagt
    22141 caaagccttt gaagtgggag gtgtccaccc agttggggtt gatgagcagg gtcttgtcat
    22201 ccactttggt gagggcggtt tttaggtgca gacaatcatg catgtccaca gcggtaacgg
    22261 tgtagccata atgatcgagc agttgctgaa tttggcgtac agcatctgcg ttgctgcgag
    22321 tggagttgcc gatgtagatg ttttttccca aaacgagcac atcaccgccg tctacggtgc
    22381 ctggctctgt tacatggaca agcgggcggt acgacgataa tgcacggatg atggattctg
    22441 tttcgggttt ccgagaatca gccccaggtc gggtgatgac cgccacttct ggcaggataa
    22501 aggctgtgtc ttccacaaat accgagtcag gcaggtcggg ctcttcgggt aattcaatca
    22561 cctgacaacc cagggcggcc agcgcgtgaa catattcctg atgctgggca cgagccacat
    22621 tcagatcgat gggggtgcgt tcaatgtggg ttacttcgca ttcgttaaag cgcggactga
    22681 ttttgcgggt gatcgctatg ggcatttagt tttctccgga gcggtagcgg tcaataagag
    22741 cttttgtgcg cgggtccttc ggattcttga agaggtcatc aggggtggcg tcttcgatca
    22801 gttcaccttc tgccatgacg atgacacggt cggcaacttc acgggcgaag cccatttcat
    22861 gtgtgacgac gatcatggtc atgccttcga gtgccacctt tttcatcacg ttcaacactt
    22921 cgccaatgag ttcggggtca agggctgaag tgggttcatc gaagagcatg acacgcggct
    22981 ccatggtgag tgcgcgggca atggccacac gttgtttctg gccgcccgaa agccgcatgg
    23041 ggtattcgtc ttttttgaaa agcatgccga cgctgtcaag gttttgctcg ccaagctcaa
    23101 tggctttttt gcgatccatt cctttgacga tgaccgggcc ttcgatcacg ttttccaaaa
    23161 cggtcatgtg ggggaacagg ttgaattcct gaaagaccat gcctgttttc aggcgcacat
    23221 catgaacaag ctggcggtga cgggacccct tttcgccgcc ttccacacga acgccatcca
    23281 cctcaatggt gccgtgagag ggttcttcaa ggaaattaag acagcgcagg agcgtgcttt
    23341 ttcctgaacc gcttctgcca atgaggcata cgacctgtct ttcaggaacc tcaaggctga
    23401 catttttcag aacgtgcaaa cggccaaaat atttatcaag gtttgatatt tttacaatgg
    23461 aggatgtcat gttatctatc tcccttggaa aggcgggttt caatgacacc ttgaacatag
    23521 gaaaggatga tggtcatgac ccagtaaaaa atggcggcca tgatcagggc ttcaaggttg
    23581 tggaattgtg cgcgtccaac tttgacggcg cgccaggtca actcgtgaac aaagcccgtg
    23641 gctgaaacca gcgcggaatc tttggtcatg gcgatgaatt cgtttcccgt gggtggaatg
    23701 gcgaaacgca tggcttgagg gaggatcaca cgcttcatcg tctgacctgg ggtcatgccc
    23761 agcgctgctg cggcttctct ctgccctttg cctacagaag ccagccccgc gcggaaaatt
    23821 tcagacatgt acgcgccgta gtttaatccc agcgcaatga cgcccgccca aagcccaggc
    23881 aggcggatat caagctgcgg aagggccagg aagaaaaaca tgatctgcaa atacagcggg
    23941 gtaccgcgga tcaatgaaac ataaaaggtg ctgagcgcat aaatcggcgg aaacgtggaa
    24001 agcctgccga gcgctgccaa taaagccaga aaagtggcga gaatgatgga aaggatggaa
    24061 atacgaatgg tttcgcccac gccgcccgca atgaacagca tgttttcctt gatgaagttt
    24121 gtatctacat tgatggggcc gctgaataaa aatacaagca acagcaataa caccccccag
    24181 gttagagcca cgtttgcacg gaaatgacgc tctttacgct gttgcgcttc aaacaacaat
    24241 tcggcttgtg ttttaggcgc gccagattct gctttggcga aaaggttcat gagaaaattc
    24301 ctccacaggg aattaatagc aaggaagcgg catcacatgc cgcttccttt tgttttgaac
    24361 agtttacttg ttcggtgcct gggtcaggtc ttcgctgaac catttcatcg agagttcggt
    24421 caggcgtccg tcaccgtgca tggcttcgaa gattttgttc acttcggcga tcaggctggc
    24481 tgtgggcagg gtggaggatt tgtcgaacgc tgcggcaagg tcttcagagt acacagcact
    24541 gccgagaaca cgaacgggga tgccggcggc aatattcgca ttcacaacgg tggctgaggt
    24601 ggagtaggcg gcaaagtcgg tgcggccagc ggcgagcgcc tgggcgcatt cctggtcaga
    24661 ttccaacgga accacagtga tgcctgaagg aggcttggcg tagatggaac tggcgggcaa
    24721 gccaatttcg ccgccattca gccaggtttc ataggtggtg gaaacacctg cacaaattgc
    24781 ctgaccagag aggtcttcaa gagcctggaa ctcagagtcg ccaagtactg caacaaccgc
    24841 gggggtgtag tagtacggaa cgctgaaatc gagcactttt tggcggtcag tggtgatggt
    24901 catggagcca acgctcatat cccatttgtc agcccagttg ccagcggtga gggtgtccca
    24961 accgggggtg gcaaaacagg tttcaacacc gagcgcatcg ccgacggcaa tggccacatc
    25021 cacatcgaag ccctgcattt cagcagtggt caatgcatca gaggggcatt tggtgtctga
    25081 agggcgtgag ccttcggtgt tcaggaagga ctggggttca taattcgggt cggtcgaaat
    25141 gaggatatag ccgcgttcct tgatggcgcc aagcaggtcg gttgcggttg agccgccacc
    25201 accacaagcg gtgagcatga gagatgccac tacaagcagg ctggccattg tcaaaagttt
    25261 tttcattatc tcctccaaga gaaataaaaa tgattggcaa tgtttcattg ccacggaacg
    25321 ttttattccg cgggttaagc atagactatt tttagcattt gtgcaaggat tttgtgtaaa
    25381 tccgtaatgg tacaattgtg tatactattt ttcggaggca ggcatgtctg taatttttcc
    25441 cagcgcagaa tggctcgcgg agttggaagc caaattgaat tctgatgaga agtaccgtga
    25501 gatcgccaaa aattgggagg gcgatatttt tttcaatatc gaaccagagg gaaatttgaa
    25561 ggaaccgctc acgttctatc tggacctgtg gcatggtgcc tgcagaaaag tggattacaa
    25621 ccccgcggcc gccacctacc caaaaccgac ttttatcctc aattcttcct acggcaatat
    25681 cacggctgtg ctgttgggta agatgaaccc catgaccgcc atgatgacca tgaagttgaa
    25741 ggttcagggc aatatgggct acatgatgcg caacgtgccg actgtgcttg attttgtccg
    25801 ctgcgcgcag gaagtgacga cggatattct gtaatttttc ttgccagctt cttttttcac
    25861 ttgtgagaaa aaagaagaaa gaattgatat atgctgccga tattaaaaat caacctgact
    25921 accaacacaa ctgaagaatt tgtcacccca aaggggtggg aacgggattt tctcggcggt
    25981 gcatctctcg cggcgcggat tttatactca accctgacaa aggaacttga cccgctttcg
    26041 cccgaatctc ccttgttgtt catcaacggc ccattgactg ggacaaacgg ccccaccaca
    26101 ggccgttttg tgatctgtgg aaaaagcccc gccacacagt tatgggcgga atcgcatatc
    26161 ggcggttttt ggggacccga acttcgcaag gctggttatg acgggttgtg gattacagga
    26221 aaagctcaaa gccccatata tctctggatc gaagaaggca ggttcgaggt ccgcgatgcg
    26281 gcgcgtgttt ggggcaaaga tgtgtatgag gcacaggaga tcgtcaaaga ggaagttgga
    26341 gtcaaagctg caagagtcgc ggtgatcggc gaagcaggtg aacgtggagt ttattattcc
    26401 tcgatctgtt gcgaccacgg gcgcatggca gggcggacgg gtctgggcgc tgtgatgggg
    26461 tctaagaatc tcaaggcggt tgcggttcac ggaaaaaaca agatcgaagt gaccgcgcaa
    26521 tacgcacagg cgcgttctga ggccaaccga accttgaaac aggataatga ggcaaagatc
    26581 ctgcatgaac taggttcatc cggtgtggca aactacgccg agtatttggg ctccatgcca
    26641 gcgaagtatt atcacaaagg ttcgttcgaa gaggtggatc atatttcggg ttcgaaaatg
    26701 gccgaaagta ttttggtcag caccagtgcc tgccagggat gtgtgatcgc ttgcggacgt
    26761 gttgtaaatt taggcgacgg agcaaaacga aaaggccccg agtatgaaac gatcgctggg
    26821 tttggcccca atcttttgat cggcgacctg aatgatgtgg ttcgtttagg ggagatgtgc
    26881 gataaatatg gaatggatgc catcagcgca tccaatacca tcggtcttgc attccacttg
    26941 tttgaaatgg gcaagataac gcttcaggat acgggtgggc tggacctgac atggggaagt
    27001 ttccttgctg cgcggcagct gcttcgaatg attgcccgcc gcgaggggat cggcgacctt
    27061 atggcgcaag gctctcgggc tttgggcaaa gcctttgacg ctgaagatga agccattcaa
    27121 gtgaatgggc ttgaaacggc atatcacgat ccgcgcggcg tttcagggat ggcgctttct
    27181 tatgccacca gtccgcgtgg tgcctgtcac aaccaatcag attatttctt tgtggatttc
    27241 ggccatacct acgacgagat cggcatcacc tattttgatc gccatgcgca ggccgaaaaa
    27301 gccgccaatg tggcaaagca tcaagactgg cgtactcatt tcaatgccgt tttgatctgt
    27361 atctttgcca atgtgccgcc agaaacacag gtgcagttac tcaatgcgca attggatttg
    27421 aattggacca ttgctgattt gatgcgttca ggcgagcgcg ggtggaatct gaaacgcgtc
    27481 atcaacaatc gcatggggct cacccgtgca aatgataaat tgcccaaggc ctttcttgag
    27541 ccgcatcaag aaggtggcgc agcgggattt gtccctgata ttgaaggcat gctttcggct
    27601 tattatgaaa ctcgcgggtg ggatgtaagg acaggtcgcc cgaccaaaca gaaattgatt
    27661 gaattgggaa tgtcagatat tgcaaacgac ctgtgggggt gacaggtcgt tttgtgatcc
    27721 aaattttatt tttcctcggt tgcgttcaac cgccagacgg ccagaacaaa aaagacaagt
    27781 gcataccctg ccagtgcgag gcagggtatt acaactgatt caaacccaag cccgcgggca
    27841 acgatgtttt caaaaccgtc catgccccag gctacgggtg agatgtgacc aatggcctga
    27901 aaggtttcgc cagtaaattc caatggcacc catgcgccgc ccaagccaga gaagacgaac
    27961 atcggtacca gcgagaaggt aatggcctgt tcctcggtgc gtgcgaggat cccgatcagc
    28021 aggcccagcg cggagataca gagcgccgcg gtaaaggcta caagcaatgt tgccgaaggc
    28081 aggcgcagat aatccacttt caaagcgaat tgaccgaaac agatcaagat gaggaactgg
    28141 gtaaagataa gcgagaagat cgccagaaaa tgacccacta tgatttgaat acggcgcgtg
    28201 tccgtggtca gtaacctttg cagtgtgcgg gatttgcgct cggtaacaat gacctgagcc
    28261 gcggtcagca ggcctgcaat ggcaaattgc agcatcatgc ctggtgaagt atgtgccaat
    28321 gcgttgttct cttcgccctc tttgatcaca agactggacg tttcttcaat ggcgatgggc
    28381 ggctcatccc atgcggctag aatttcatca aaggcgtaat taaaaggcac gccttccatc
    28441 ttaaattgct ccatgatgcc agcggtacgc actgctccat ccacacggct ggctgcggtg
    28501 aatatttcag cctcgatcgt catccaagcc tgggtgtttg tatccgccag cactgtgagg
    28561 cggatcgttt tatccttcaa cacagctctg ccgtacccat cggggacaat gatggcggcg
    28621 gcaagttccc catttgcaac cagtttttcc aggtcagagg cggaacggaa cacattttca
    28681 tccagacgaa tcacttcaga gtttttgagt agatcgtgca attcctcggt cacccagctg
    28741 tcatccagag agagatatcc aacaggcacg cgcgaatcgg attcgttgcc gccgaaccct
    28801 ccaaaggcgt atccgaacaa aaacgtgaaa aggatcggca tgatgagcag gaacatgaaa
    28861 gtcttgaagt cgcgcatcaa ttgcagcagg tcttttaaag caatgtcgag aatgcggatc
    28921 atgtcgggct cctatgcgta ccgcttgttg aaacgccata cgccgatggc aaagaatgcc
    28981 gcaccccaca aaaacgagac cagcaccgtc agggcaatgc tgttgaagga ttcgccgctc
    29041 atggcttgca tgagtccgcg aacggcccag ccctgcggca ccagcagtga aatggtgtcg
    29101 cccattttgt tggcaatggc cgagttcatg gcaaagatct tgatcatgcc catcatgcca
    29161 gtcaccgtca aaataccgcc gaataacact cccccctgtt ttgtatcttt caaaaatgaa
    29221 tttacaaaaa taccaaacga tgatgcgctg acaataattc caaccgccat caatgccact
    29281 gatgtaaatt caccccattg gattccaaag atcaaattcg cggcgatcaa cagcacgatg
    29341 atctgcaccg aaacggtcat gaacactgaa agaagtttgc ccgtcaaaat ggtggcttgt
    29401 gaagtggggg tggtaaagag gcgcggaagt gtgcgctctt cttcctctct aagaatgctt
    29461 tgcgcggtgg atgtccctgt gtagtaggca tagaaaacca tcatcccacc catgattgga
    29521 ccaataatgc ctaataatag attctgactt tcatccgctt ctttttctgg accgttggct
    29581 gggttgcgca cctcaagcag ttccgcctct ggatcatcgc tttctgccag tgaaacttcg
    29641 agatattggt caaccacttg gcctgtcaaa acaagctgtt cgggctgtgc ttcatccatg
    29701 aagaagttga tggcaatctt cactcccgac atgccgtcca taaaacgatt gaggatggat
    29761 cgcatcacag cgggcccgag ggtgagcgtt gggtcttgat aaaactcgat cacagctttt
    29821 ccatccacat cggcgaattg atgcgagaaa tcggctggaa taatgatggc tacctgtgat
    29881 tcctggcggt ccaccgcact gcgggcggat tctgcgtttg gtgccagaga gacttcgatc
    29941 aagtctgcca tgtcatcgct ctgcagaatg ctgacaatga gatcgcccat cgagtcggct
    30001 tcttttccac cagggatgtt tttcgggttc acttggaact tcggcccgcc ttcatcaagg
    30061 ttggcaatga tcacattggt tttcggcagg gtaaatccgc cttcgtcggc gatgttgcca
    30121 aacataaaat aaaacatgcc agtgaccagc agcggcacgc cgaacataaa cacaacggcg
    30181 aatgtgctgc gaaaggaacg ggtcatgtct ttcagggcga tgtcgaatgc tttcatgaca
    30241 aaattcctta atcgcgaagt gcgcgccctg tcaggtgcag gaaaaccgcc tcaaggttcg
    30301 gctcgctgat gttgaccgaa gtgatccgtg cagaatattt tgtagccgtt tcgaagaggt
    30361 gaggtaaggt caggttgctg tcttgaacca ggacgctgat caactctccg ttaagggtga
    30421 tgcgggaaac gtcagccagt tccttccagg cggggatcac ggtatccatg tttccgttca
    30481 gcgtcaggtc aatgcgtgtt tgctgaccga ccattttgac cagttcatcg tgggtgccgt
    30541 gtgcgatcag ctgaccatga tccataatgc cgatgtggtc agaaatctcc tgtgcttctt
    30601 ccatgtagtg cgtggtgtac agcacggtca tgccttcgtt tttcaacaat atcacgctgt
    30661 cgaggatgtt gcgccgcgat tgcggatcaa tgcccacggt gggctcatcc atataaatga
    30721 ctttgggttt gtgcaaaagc gccacaccga tgttgacacg ccgtttcatg ccgcctgaat
    30781 atttcccaac ccgttcgctg gcgcggtcac gcaggccaat gacgtccaac acttcatcaa
    30841 ctcgagattt gagggcggtt ccacgcaggc cgtacatctt tccccaaaag accagatttt
    30901 cgcgcgcggt caggtcttca tacaaggcga tatcctgcgg caccactccc agcactgatt
    30961 tgactccgcg ctggttcgtg gaaatggaat gccccatgat ttgtgcgtca ccttcatcgg
    31021 ggtgaagcag gcaggagagc atggagatgg tggtcgtttt gcctgcacca ttggggccaa
    31081 gcaggctgaa aatctcacct tgatctactt tgaagctgac accttgaact gcatgatttt
    31141 ccccaaagga tttgcgtagt ccttgaactt cgatcgctgg cacaatggtg ttcattagag
    31201 gctctcgttt tcagcgatat atccttatcg cggaatttgg gagtggctaa aaactgggtt
    31261 ttgatttcca cggaatcagc cactcgtcgg aagtaaaact gttctttcta tacgcagatt
    31321 tgcggaattc gtttcacttt ttcagccaat ccagagcact ttcgtaatca acaaagagcg
    31381 aggcgaaaaa gccgttattg cggcagacag tttcgtaaaa cttcaggttg gtttccagtt
    31441 ttttaggcac aatcagggct attttaaatt ggcgcggaat cccaagctcg gcatagcgtt
    31501 tgggaagctc gtaaatatcc atggtggcga gcgcatcttc cgaaatgtcg cgatggtcga
    31561 gcaggcatcg catacagttg tgttttctga taagttcaac tccttcgttg cgcatcaaag
    31621 tagcggactt gatctcaagt acgccattgg ttttaatgaa aatgatcttc tccgcctcgt
    31681 catagtacat tttccaattc atggctgcct ctctccctgt ttttattcct gacgatattg
    31741 ttattatata atagagttct tgcaaaagtc catttttgcc agatggtctc aaagacttta
    31801 caagaggtcg gctggtttat cgcggcaatt cccaattaac cttggccgat ttggggcaac
    31861 tcactgatcg cctgaaataa aattggaggt tgaatgaaac tcgttactgt ggctcagatg
    31921 agagccattg aaaaagaagc agatgccaat ggggtttcgt atgcacagtt gatgcaaaat
    31981 gcagggcgcg gccttgcaga agtggtccat gctttggggc aggagaatgg ctgggaggaa
    32041 gttacagcct tggtgggagc aggcaataat ggcggtgatg cacttgtcgc tctcacttgg
    32101 cttgcgcagg caggctggac cacgcatgca tatctggtta ggcgtgattc ggccaacgat
    32161 gacctggtca cggcgtattt ggcggctggg ggagaagtgg cggattttga agacgaccga
    32221 gcgctggcgg tttttcttaa tgagtcagat gttcttttgg atggcctgct tgggacgggg
    32281 atcaggcttc cgcttaagaa agaggcggcg cttgtccttt caaatgcaaa agagatgatc
    32341 tctgaaatgg aaattccgcc ttttgtcgtg gcggttgatt gtccatctgg cgttgattgt
    32401 gacagcggcg aaatcgccga ggagaccctc ccagccgacc tgacgcttac gatggcggca
    32461 gccaaatacg gattgctcaa attacctgca ttcgaattta tcggcgagtt gcaagtcgtg
    32521 gatattgggc tgcctgatgg tttgagttcg tggcaggagg tcaacaccga ggccgcggat
    32581 gatgaacttg tcgctgtgct tttgcctgaa cgtacccccg cttcgcacaa agggacattt
    32641 ggtacggctt tcgttgtggc tggttccacc tcatacagtg gggcggcttt gttggccgcg
    32701 acagccgcat accgtatcgg tgctggcctt gtgaccttgg ccgtcccgga gcccttgcac
    32761 attgcccttg ctggccgatt gttggaagct acctgggtgt tgctgcccca tgaaaatgga
    32821 gcgatttctg aaaaagcacg cgaccctgtg ctgaaaaatc tggagcgtgc cacttctgtt
    32881 ttgatcggac ccggccttgg tgattttgct tcaacaggga aatttgttgc aggtattctg
    32941 cctgccatta agttacccat ggtcatagat gcagatgggt tgagacacat tgcccgcatc
    33001 aaggactggc ataaaaagtt gttcgatgtg gctgtgctta ccccgcatcc gggtgaaatg
    33061 tctgcattga ctgggttatc cacggaaaaa attcaaggca accgtgaaga agttgccgtc
    33121 agttatgcaa aaatgtgggg gcatgtggtt gttttgaagg gtgcatttac cattgtcgct
    33181 gccccggatg ggcaacggac cgtcatccct gttgcaacgc ctgcattagc tcgtgcaggc
    33241 acaggagatg tgctggctgg gttggttgct ggccttcgcg cgcagggact ttccgcctat
    33301 gatgctgcag ttgccggagc atacatccac gcccgtgcag gaatacttgc cgctgacaca
    33361 ttgggcacca ccgcctctgt actggctggc gatgttttgg atatggtttc agatgtcctg
    33421 gctgagttgg aatgacaaag tggtaaagac ccatgaactt atcttgccaa tggcaaaaag
    33481 ccaggcatta aaacaaactc accccgaaaa attatttttt cggggtgagt ttgttttttt
    33541 attgttactt ttttagctat tcatgaaact ttcaaggttg tctttgctga tgcgataagc
    33601 cttgccaacc ttcttggcct tcaggctgcc atccttgatg gcttcgagga tgtcatcctc
    33661 ggttactttc atcacctggg cagcttcagc gggggtcata atgtcggcga tctttgcacc
    33721 agcggcgccg ccaccagagg tgttctgcat gccgccctgc aaagcctgac ccatcaaatt
    33781 gccgatcccc attccagcac cgatgccagc ggtcagacca gcaccgccat tggcgttgtt
    33841 ggcagcttcg cgcattgcat cggccgcctg caattgagca taaaggttgg agtcgagtac
    33901 ggcgcgttcg cgcagttcct gtaccgctct ttcgcttggc ttgaggctgc caatgtagaa
    33961 ggtcttcaaa gtcaaaccaa tggcttcaaa atcatccttc gccttggcgc gaacacccgc
    34021 gcttaactct tcaaacaaac tgcttaattt caccgtaagg ttggtggttt gcgcagtctc
    34081 gccaagcaag tcacccagtt ttgagacaag catgtcgcgc aaacgaccct cgatctctga
    34141 agaacgatac gatcctgttg cgccaacgat ctgggtaaca aactgttgcg ggtctttgat
    34201 ctggaaggaa taagttccgt acccggtcaa tacctgccag ccgagaccag tgcccggggt
    34261 cggcaccaaa atggaagatg aggttcccca ttttttgttc gcaaattcct tgcgcgaaac
    34321 aaaatacact tcagcaggga agggggtgcg gtcgttaaac gcctttccga taaaatcgat
    34381 cagctgtgga atgtttgccg tggcaattgt atggcgcccg ggcttgaaca catccaaagc
    34441 ctgaccatcg cggaagaaca ccgcgctctg gctttcacgc acgatcacct gtgaaccgat
    34501 gcgaaaatca ccgaaccctt cttcggggaa gcggtggacg atctcgtcgt tcatttcatt
    34561 tgggtattca atgacatcaa aaattctagc catgtttctc tccttttagt gaataacgtt
    34621 cagattatag tccaacagac tttcggctac aagaaccgtt tttcctattt gttttacgaa
    34681 ttccaacctg aaacgtttca gggcttaagc acatttttca acgcccatgc atacccaact
    34741 caaaagcaga cgcctctctc ttaacctgtg gcctgcttcg ccttcattgg cattcccgtc
    34801 gcctgggctt ttaccatcat cacctcagcc agaatgctga cggcgatttc ctcaggggtc
    34861 tccgcctgca actccaaacc gatcggcgag tggactcgtg cgatcttttc ctctgaaacc
    34921 cctttttcct tcaatgcttt cacggtggtt gcccaacgtc gcctggatcc gatcacacca
    34981 atatacccag cggtcgaatc cagcaatggt ttcatcccga ccgcatccac actggaacca
    35041 cgggttgtca gaattaagac agtgcgagag tcgattttga tgtgttcagg gagtttgtcc
    35101 atggtaatgg ggtggtagat atcagcccca ggtgtggctt caggggtgca aaactcggca
    35161 cgatcatcgc acactgccac acggaaaccc agccacttgg ccaaatgcac aacagctttg
    35221 cctacatgcc cggcgccgat aaccacaagc aaaggcgggg gaaggatcgg ttccacaaaa
    35281 acctccactt gaccgccgca taccccgggg tcaccgcgtg aagggtcggc catgttgtaa
    35341 tgtaaatagc gtggctttcc atccccgagt gccatccacg cctcatcaag gacacgatgt
    35401 tcaagatctc cgccacccac ggtgccgata aaatttccgt cagggtacac cagcattttg
    35461 cttccaacat gtcttggtgt agagccttcg ctctttacga ccgtgcagag cgcggcagag
    35521 ccgttgttct tttcgagttc aaataaagcc tggtgaatgg atgcagtcat ttataaatac
    35581 acgtcccgta tcaggatatt ttttggattc ctttggtcaa cgatcagagc cagttctttt
    35641 ttctcgagca tgagtttggt cttttcagtt ataagctgct tcaatttcac atcgtgggtt
    35701 tgaccaaggt gtatgtaatg caagtggagt gtccagacgg ttctgaaaac agagcttttg
    35761 agtatttggg cattcacttc aatgccatgt tcaaaggttg ctgtaacata gggtacgcgt
    35821 accgctatga caatgatgga atagatgagg gccgccaggg tcaaccagat aaaattcagt
    35881 ggattgacgt tggctggcac gatgcctgtc aatgccttcg tcacataaat gattcctgtg
    35941 acggcaatcg tgaaaatgag accgcccatg ctggaggctg ttgctgaatc attttttaga
    36001 atgctgaaga catctggctt ttggatcatt atctttccaa tagacctaac acagctggta
    36061 aaagttgttt ctttctggat accactccct gggcatcgcg agtaccatcg gagagcggcg
    36121 gataggggag gtcttccagg atggtgggcg cattggccga aatgagcagg cggctggtgc
    36181 cgcggactac atcggtaacg aggagcatgg caaagtgtaa accacgtttg tcgcgcaggt
    36241 catttaaggc gttttgcagg ggttcaaggt gttcggtcag ttgcagaata tcggtgactt
    36301 ctgcttgagc cacggcaaat ttgaatccgc cgccttcgaa gggtttgatg tctgtactga
    36361 ccacatcctt cgggtcacgg tttgaaagcc ctgcgcctgc gcttagaaca gccttgccat
    36421 aagattctat ggtttcgcct ttgagcgggc tcccgccgac aaatgcccag cgggataatc
    36481 ggtctgctgc cactttgtca cgctgcgtgg tcgttggtga tgtcaggatg agagtatcag
    36541 aacaaagtcc agcaagaagc gtacctgcca atgcaggggg catggagagg cccgcttcag
    36601 cggtcatttc ggccaccaaa gtggaagttg atcccactgt atccaccgta aaacggattg
    36661 gttgatgggt atagggattg ccaaggcggt gatggtcaag aatttcaagc aattcggctt
    36721 cttcaagtgc ggcaatagcc tggcggggtt cgttatgatc caccagaatg attttcaggc
    36781 gcggcgggtt aagtacctcg cgctgattgg caatgcccac gtatacaccg tgttcgtcca
    36841 ccacccaata atcgttgaat tcgtcacgca ggattttgtt gagcgcatcg cggatgtggg
    36901 cgttggctgc gtatttgggt acgctggtat ctgcggcatc cttgcattgc gccgccatca
    36961 tctcacggac agttgtgtcg ccggggcgtg ggccaagggt ctcgctgaaa tatttgaaga
    37021 ggctcatccc attgatgatg ccaaacggct ttccatcttc attgactacg ggtgcaacgc
    37081 cgcccgtttt gctggcaaga gtccatgcca ttccaagctg cgaatcaggg cggatggtgt
    37141 ccagtcggcg catgaccgac tcgaagcggg gcgatgcatc tgtaagtaaa acgggtgtct
    37201 cgaggccgac tgttttcagc acccaggagg tttgggcatt aagagcgcca gcgcgtgctg
    37261 cgattgtgtt tgcaccatcg cgttcgcgca agagccaggc gtagcccatt gcagcggcaa
    37321 tggaatcggt atcggggttg acgtgtccta tgacataaat ttgatcaggc attttttctt
    37381 ttccaatgaa tttagagatt aagttgttgt tccctgtagt tggggattat tgtgggcaga
    37441 ttttgaagaa agtttcgaag aacgcaaggt aattatatct cgccttgttc aaccagcttt
    37501 aagaagatat taacgacccg tgggtcaaag tgttttcctg tttgctcgcc aaaatatttt
    37561 agtgcttgtt cacgcggcca ggctttattg tatgggcgat ctgatgtgac tgcatcccaa
    37621 acatcggcaa cggcaaagat gcgggctgcg atgggtattt cctcgccttt caagccttgc
    37681 gggtagccac ttccatccca tttttcatga tgagagtacg ggatatccag tgcctttttt
    37741 agaaatggga tcgggctcaa gagtttggtg gcaatttcag ggtgtttttt tatgatctcg
    37801 cgctcattat ccgtcaattt gtcgggtttg tggagaattt catctggaac tgccattttc
    37861 ccaatatcat gcaaaattgc gccgcgtcgc atgtgttcaa ggtcatcacc agcaaccccc
    37921 atggctactg cgattttcag ggtcatatct gtaacgcggc gggaatgacc ttcggtttcc
    37981 ttgtctcgta gttccaatgc gcgcgcccag ccttccagtg tggtgtcgta ggcatcggcc
    38041 aactcccagg tacgggcgcg gacacggtct tccagttctg cattcagttt gcgaagttcg
    38101 cgctcaagtt ccttttgggc ggtaatatca tgcgaaatgt agattcggcc tgtcatctct
    38161 ttgttggagt tgtagagcgg ccagacggtt aattcataat aaacatgtac accgcccagg
    38221 tcaaaggtgg cttcggcacg ggcataggag gtttgcatgt attgtttaat cggaatgggg
    38281 aaatctgcga atattttttt ggctggttcc ccgatgacat catcagcagt cttgcccagc
    38341 agggccagca tggacaggtt gatgtccaca atgtcgtttt ggttgttgag aataaccaca
    38401 ggctcgacca tggcctcaaa tactgtgtca cgtgcaatgg gggtgacgtt gaacatgcgg
    38461 aactggaata gcccccatac aacgaaaagg ttgccaatga caaaggtgaa tggagccatg
    38521 tctcgctgcg gcgtgatgcg tatgtcaaac agggtcagcg ccatgccgag gatgggaatg
    38581 agcagcccca gcgagatcat tatcatttga gtttgataca ggctgtgaga atgggtaatg
    38641 cgctgaaaca aaatacgaag tccccatgcc aaaatcaaaa gtgcgtaaaa ggcatatccg
    38701 taaacgagcg gcgtgaaatc gtacagcagt tctgtaaaaa aaccctgggt ggaaaggtgt
    38761 ggattcctgt aaacccaatg caggttttga tccagaaaca gaacactgga aaacaggatg
    38821 gggacaataa aggacaaccc aaaaagcagg cgtggataat tgagcttata ttctgtgtat
    38881 tggaccagaa atatcggcag cgagatcaaa ataaagaatc cagcaaacca ttggaaggaa
    38941 tcccaaaaga tctttgtaga gatatcgctg gaggttagtt cgcagatgaa acctgtcagc
    39001 cagagtgact gcccaagacc gtaaaatgag aatgcaaaag cccctcgagc ttttcgcctg
    39061 ctccaggtgt agacgaagac ggcaatcgaa acagccagcg agacaaggta tgggataagg
    39121 tgaggaattt cagaacgggt catgaggatg atagctttat tgtatcaacc caatgagttt
    39181 catgggtgct ttgttttacc gcggtttgga aacccttttt aatataataa aaaagcagat
    39241 gcaaatccag cgtgcaattt gcatctgctg ctgtttagct gggggctgat gtttgagccc
    39301 tgagggcctt ccaaactttt tcaggggtga ccgggttatc gtccacgttt gcaccgatgg
    39361 catcgagaat ggcattgccg acagcgggtg cgaccccgtc catggggatt tccgccacag
    39421 ccttcacacc aaatggatga ctcggctcga aagtttcgac aaagatggtt tccaactcgg
    39481 gcatttcatt cgcgcggaag agatgatagc ggtcaaaatc ccgctcgatg gcatagccct
    39541 tttcgtcgta ccgcatctct tcacagactg cataaccaag agcctgcatc ataccgcctt
    39601 cgatctgccc ggaggccgtt agcggattca cgatgactcc agaatccact gccatcacga
    39661 gtcgatccac tgtaacggcg cccgtttcag tatcaacggt cacttcagca aattgagcag
    39721 caaacggagg cggcgaaacg ggtgagacgt agcttgccac acccatgatc tgttcttgat
    39781 tgtttttgtg cagtgtgtcg agcgcaattt ctgccagcga aacggacttt ccgttcggcg
    39841 cgtgtgcgtg acggttgcgt aattgaatgg cgttatgatc ttccacaccc agcatggtcg
    39901 ccgcacgaat cttgatccgc tctgctgcga tctgtgcggc ttttgtggcg gcagtgcctg
    39961 agatataggt tgtggaggat gcgtaagcgc ccttgtcgaa tggggtgaaa tcggtgtcgg
    40021 atgaatagca gataatgtct tccacgggca cgcccagtac ttcggctgcc atttgcgcca
    40081 ggaccgtatc ggagccagtt cccaggtctg tggcgccgac gagtagattg aacgacccgt
    40141 catcattcat cttgatggag gcaccgccca tatcaaggta cggaatggct gtgccttgca
    40201 tcaccatcgc aacgccgatg ccttttctct ttgaacctga aactgagctg cgccaacctt
    40261 gatttccaaa cttgtcatcc cagttgatgg cggcgcggcc ctgggctaca cattgctcaa
    40321 gacccacggt gtgaatgatc tcgggtcttg gttcgcggcc ttcattccat gcggttgaga
    40381 acggatgata ctcaccaggg tgaattgcat tcttgaggcg gaaatcgatc gggtcgaagc
    40441 ccatggcacg cgcgatcttt tccatgtgac ggtcgagcgg ccagtagcct tgcggcacgc
    40501 catacccgcg gaatgcgccc gcaggcggtg tgttggtgta aaccacatct gcataaaaac
    40561 ggatgttggg cgctttgcga tattctccat cgccgacata caaagccatc gccttgtgac
    40621 ctgtgttacc tgtcacggtc aaggcatggc agccgtatgc gcctgtatct gaaagcgcgt
    40681 acatcgaatt ggctgtgatg gttccatctt ttttcacgcc tgtcttcatg cgcacacgca
    40741 tggggtgacg tgaacgcgcc gcgatgaatt cctcttcgcg ggtgtattcg taaatgacag
    40801 gccgttttgt ggcaatggtc aaatgcgcgg ctacatcttc catcaggact tcctgtttgc
    40861 cgccgaagcc gccgccgatt cgcggcttga taacgcggat gcgtttgacg ggcaaaccca
    40921 gtacaggtgc caacattctg cgcacatgga aaggaacctg tgtggacgtg cgaatcacca
    40981 atcggtcatc ctcgtcccaa taggttacac agacatgcgg ctcaatgtgg gcttgctgca
    41041 ccttcggtac ttcgtattcg gcttcaaaaa tttcatcggc ttcggcaaag cctttttcaa
    41101 catcgccgat atcaatgcgg atatgtgccg caagattttt ctctggattg gaatccgcaa
    41161 agttgacata ctctggctcg tcatgaatgc ggatcggact ccccatggcc tgcctcgaat
    41221 caaggatcgg agtcagcacc tcgtattcga cctcgatcaa cgaaagagct ttttctgcaa
    41281 tttcaggtga ctcggcggcg acaaaggcca cacggtcgcc cacaaagcgg acctttttat
    41341 cgagtgaaaa cgcatccaac ggcccgggga tcgggtctga ttggcctgct gtggaataga
    41401 ccacgcgcgg caggtctttc catgtcaata ccgcggcgac cccttcgagc gcgagggctt
    41461 tgctggcgtc aatgtttttg atcagcgcgt gggcgtgcgg cgagtgtaaa acttttgcgt
    41521 ggagcaatcc gcgtttttca aaatcggcgg caaaggcagg tttgccttgc accagtttga
    41581 ccgcatctaa tttaatttcg ggtttgccca ctgtttgcca ggtctgtgtt tcgggtgtga
    41641 ccactacttt gggccgaacc tgcacacccg tcaggtttga ggcgggggcg tcattttgcg
    41701 gagaatcatc tttgggcgtt ccgaaatccc agttgatgga attccaatcc agcggcgtat
    41761 cgcccctcat ttctgcagcg gctttcatca cggcttgtac aggtttgaga taacccgtac
    41821 agcgacacaa cacacccgaa atggcttcac gtacttccgc ctcacttgga tttgaattct
    41881 tttcaagcag ggctttcgca gcgaggattt gcgcaggggt gcaatacccg cattggatcg
    41941 cgcctgtttc aacgaaagcc ttttgaagcg gatgcaaacc atcggtggtc ttccagcctt
    42001 gttcgggatg ttcgccaagt gcctcgatgg tggcaatgtc gtggccttcg gcttgtgcgg
    42061 cgagaagcgc ccccgcgttg acaggtttgc cgttcagcag aaccgtatct gcgcctgaga
    42121 ggttgccctc atctccaaac ttgactccgt gatatcccag cccgcgcaaa gcggcaagca
    42181 gggtggtggc tggggcggta tcaatggcgt gctcaattcc gttgactttg agtgtgatgt
    42241 tcatgtgaac tccttgattc accgcagaca aaaagatatc ttcaatgcat ccgtatttat
    42301 ctgtggttga aaatgtttac aaaacctttg taactttcaa gccagctttc gagccttcga
    42361 tcgcagcctg tgacccgatg actgcaaccg cttcagattt ggcagctttt tcaagtacct
    42421 caccaaaaga gatgaagtct tcgccgttgg tggagaggat ctcatcacgt ttcttttgaa
    42481 gcatggcatc ggttttgccc gtgaggtggc gcatcatgga tgtgtaacct ttggcgtcgg
    42541 gcagctgata ggtatcgagg tcgccaatgg cggcgatgat ggccttggtg agttcgttat
    42601 cgttgaagag gttcggatcc agattcttca agtacccagc cgcattgtcg taattttcga
    42661 gggtagtgtc gaggttgggg tcgcggtacg agaggaagga aaatacgcca gagttttcgt
    42721 cgaaaacgca gaatccgcca taggccccgc ccagtacgcg gatcttttcc caaaggtagg
    42781 ccatgcgcag gtagcctgtg accacatcag cagagccatc gaattcgtag ccaaggtcgt
    42841 agaggttggc agccttgccc acgtaattga cctgtgcggg gatggtgagg ccttcctttt
    42901 cggggtgggc tttgatgtcg aacggtttga gcgtcacttc cttatcaggc agggcaaaga
    42961 tgaagttttc aaggcgcggt ttgaaggcgg cccaatttgc cgcatccagg gtgacgttgc
    43021 agatgagtga cctgctgttg atgagggcgt cgcgcatggc ttccagtttc ttcaaaaccg
    43081 acggccactt cttgtcgatg tctttggcga gttcgcgcag ggcaaagaga tagccgatgc
    43141 cgctcatctg gtcgttgacc cagcctgagc cgccaaactg tgcgcggacg cggcggttgg
    43201 cgaagatatg cccgcctggc accagtcctg attccacgcc cgatttttcc tccaacacga
    43261 tctgtttgag gcgttcacgg ttgtcgaatt ttgtggtgag cagaatgtct ttcaggatgg
    43321 cgagcatctc aggcgcttga ctcatcgtgg ccttgccgcg aatgaacaac ttgctgacgt
    43381 tctctcttga cccgtaagct gtggtgctga gggcgtcacc gtagattccg cctgtgcttt
    43441 tgccgatgcg ttgcgagagt ttgacgtaat cttcagtctc ggttcccatt tctagcaggg
    43501 cgcgggcaaa gatctcggtc agcggcaggt attcctgcgg catggcgtgc aggtcaaagc
    43561 cgagatcgag ataaacaatg ccgttggtga acaggtcatg gtacagcacc tgtgtatcct
    43621 gtagttgcac gatttcgatg gggatgggtt tgttctcttt atcgaggtct ttcaatttca
    43681 acacaggcag ggtggccacc gcttcgggcg cattgggtgt ttcctgcatg agcttgagtt
    43741 ttctggtgtt ttccaccatc tcggcgatct gttttgcatc caacgattcg cggatctttg
    43801 ccaatcgcgc tgactcttct tcttcgaggc ggcgggcaag ctcggggtcg ggtttgaggc
    43861 gcagtgttgt gcggtgttga ttctccaaca ggtgagtctg aatcagcctt tcgaaatagc
    43921 gcgggtcgga ggcgagtcgc gctttgattt cattgagagt ggcttcgaag ccaagcaatt
    43981 tgaagggatc ttcgtcgtcg tggatccagg tggtgaggac gcggcgcatg agcgcaatgc
    44041 cgcgcgggaa tgagcctgtg ttattttcgc gcaggcggaa ttcggccgtg ttgactgccg
    44101 cggtgatcat atctgggtca aggccatcac gcacgagtgt ttcgagggtg tcgagaatca
    44161 atctctcgat cttgcgcgca tggcgggcgc gtgtgccttt cagacccgtg gagaagatca
    44221 ggtgacgtaa ttcggtttcg aggccgaggc ctgcaaggtc ttcgccgagg cctgaatcga
    44281 gcagggcctt tttgagcggg gaagcaggag tgccgatcaa aatgctgccg agaatgcgca
    44341 gactgaagtt caaaacgggg tcggtggttt cgggcagggt ccaattgacc acaaggtaat
    44401 tcttggcttc gatatcggtg tcgctgcctg cgtcgtagct ggattccact tttttggcgc
    44461 gcttgaacgg cttggctggc ttcacgcttg acttcacttt tttgcgcttg tacgatttta
    44521 ggtagccttc catcaatttc aagcgggtgt cggggtcatc atttccgtaa aagaaaatga
    44581 acgaattgga tgggtggtaa taattttccc aaaaggcttt gaaatcggca taggtcaggt
    44641 cggggatgtt gcgcgggtca ccgcctgaat cgacgctgta gatgtgtttc gggaaaagcg
    44701 attcgatgat gcgggtttca agcacgcctt cgggtgaaga gtaggccccc ttcatttcgt
    44761 tgaagaccac acccttgtag gtaagcggct ccgcaggatc gttgatctca aagtgccagc
    44821 cttcctgcat cagggtttgt tcagtgatga gcggatgcag aaccgcatcc acatacacat
    44881 cgatcaggtt gtagaaatcc tgcaggttcg ggctggcgat cgggtaacag gttttatctg
    44941 gaaatgtgtt cgcgttgatg taggttgcca gcgacccttt gagcaactcc acaaagggtt
    45001 cttttactgg gtatttctca gaaccgccca gcacagaatg ttcgaggatg tgtgccacgc
    45061 ctgttgagtc ttttggcgtg gtgcggaagt tgatgctaaa taccttgttc tcgtcatcat
    45121 tgatgacgga aagcaaccgc gcacctgtgc gcttgtgtac gtacagcttt gcgatggaat
    45181 ttaattcggg aacctgttct tctttgataa gggtaaacgc tttagacatt atttctcctg
    45241 tgtgatagtt tactggtttg aaggtttgaa cgtttaatta acctggcaac tttccaactt
    45301 tcgaacctgt caactttctg gttttttctc gtaattcgtc gatcgctttc atttttccat
    45361 gttctttgta caaccataaa tcccacccat tgacggggcc gtttgcaagg gcctttgcca
    45421 gagcgtggat cgagccaacc tgttcctgat atttcaaatg cccatttgac aaaatgacgg
    45481 cgcgaatgga tggatcgttc ttaaagaaca acttttgccc cggtgacagg aatcccgcct
    45541 caagcagcgc tccaaacgga acccgagcct gattccgttt ttcaggcaga gtcagcgcct
    45601 cggcagggga tttcgtcact gcggcgatgc gtttctgcgc gacctgaatg tatttcctgt
    45661 cgcgttcgat gccgatgaaa tcacgtccaa gctttttggc gactgcgcct gtggtgccgc
    45721 tcccaaaaaa tgggtcgagt accaggtcac ctgccttggt gctggaaagc agaacccgat
    45781 acaaaagggc ctctggtttt tgggtcgggt gagcctttgt cccgttggat ttgatgcgct
    45841 ctttccccgt cgccagtgga atatgccaat cgctgcgcat ttgcaagtct tcattcaggg
    45901 ctttcatcgc cttgtggttg aaggtgtatt ttgcgccctt tgtcttttgc gcccagatca
    45961 tggtttcgtg tgcattggta aaacgcacac cgcggaagtt tggcattggg ttcgatttga
    46021 gccagatcac atcgttgagg atccaaaagc caaggttctg catgatcgcg ccgacacggt
    46081 agatgttgtg atacgagccg atgacccaga tcgttccatt ttccttgagc accctgcggg
    46141 cagcgccaag ccattcggat gtgaattcat cataggcggc aaagctggaa aacttgtccc
    46201 acttgttgtt gaccgcgtcc actttgctga ggttggggcg gtaaagatct tgctgtagct
    46261 gcaggttgta tggggggtct gcgaatacca aatccaccga attctcaggg agcgcgttga
    46321 gaacctcgat gcagttgcct tgcaaaatct ggttaacggg tagcctggtc actgctcaaa
    46381 ttatactccg cccgccccat ctggatggat ttttgaggct tttcaggctc tccctcgtcc
    46441 aaaattacca ttgtggtaaa ccccgatctt gtggaaaaat ttatnnnnnn nnnnnnnnnn
    46501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46561 nnnnnnnnnn nnnnnnnnnn nnnnctccct cgtccaaaat taccattgtg gtaaaccccg
    46621 atcttgtgga aaaatttatc atattccata tagaggccct ccatgaacga aaaatccagt
    46681 gtgtctgcac cgatcaaccg actttcgcct tctgagttga cagataaaat tgcacaagcc
    46741 aatgtctttg acctcagcgc tttggagcgg aatcgcatgg ggagactttc tgtcagtcag
    46801 ttttttgttt ctcttgccgt tgtgggaaat gccgttttta atgtggctgg cttttttatg
    46861 ttgaattatt ttattccggt ggggttcatg tcgctgcgat ccaattcatt cttgaaggct
    46921 gctgcctgct ttggagtttc catcgctgtg atcgccattt ttttgtggaa gggaaagaaa
    46981 ggcgcttcca ttggatttga ccgttacgat atcaaggaca ttacacaatt ccctctcaag
    47041 agtcttctga ttattgacct gttgctttgg cgggtagcgg aatatcgcgg gtctgtttcc
    47101 agtgataaag cgacgggcat cgttcgcaca aaggattatc gcgatccaga taatccaacc
    47161 agggtcaacc ctttttctga ggaacaaacg gtcagttatt actcgtacaa agcgggcagg
    47221 gataagttcc ctgtttcatt tgaagcgtac aaagctttgc agggaaaact gcaagattgc
    47281 cgcctttatt acctcccctt aagcaagatc atgatcaata tggaagtttt gcagttgtga
    47341 ttcttgaccg taaatataaa atcggtcgcc tgttttagat tggttaattt tccaaattat
    47401 gttcagaaat ccctgctttt gcaatcagta cattaggact tcactcattt tgggagtttt
    47461 gaaatgcagg acaatatatg atggggaagt tgtggcagtg ccccctgtta tacttgtctc
    47521 atgttgtcac ttggtaaact tggaaagcat gaacatggag tagacattca ttatgcccta
    47581 tgcataccga cttgacaaag atgtcaaaat tttctatctt gtcacatacg gccattgtac
    47641 ttttggtgat attttcagtc cctcgctgac accagaactc aacccggcaa atcggccgag
    47701 agtaaagttt attatcgaca ataccctggg cgatcttgaa gtggatatgg agggaatgag
    47761 atttttcatc aaaagcatgg aagatctgaa gaagacgggt tttaagctgg agcccaccgc
    47821 tttgttgacc accaagaaaa gcctcgaaat tttcattaaa tcccttgaac tgcttgtgga
    47881 tgacgatatt ccgacccacc tggcgtttgc ttcgctaagt gaagcgcttg tctggttgga
    47941 tgaagttgaa aatacccagg cgattcagcg tatccgtgat gaattgctgg gaaagttgcg
    48001 ctcctagagc gcccacccgc aaaaaaattc gtcaataacg cctcagacca ctcttcgacg
    48061 tctcgatcaa actcacaaag atcatctacg ccttcgtttt ttcacttata agttcctgct
    48121 gtgctttcac caacagagga tgaatgtggg gttcataaaa gccgctctcc cacttgctct
    48181 catcccagag cggaaaccaa cgtacttctt ttagccgatt tgttcctcct gcttggccct
    48241 caaaaccaag gcccaactct cctccgatcg gcgtggcaag aaacgtcaga tatttttgat
    48301 agtgatatgt tctttcgcga tcttctattt caaacagtaa tcgctccacg cggacaagaa
    48361 gccccgtttc ttcacgtgtt tctcgaatgg cggcttgctc aaatgtctcg ccttcttcca
    48421 gtccgcctcc aggcagccac caatagatag ttcctttttg gtggtttgcg tgacaaatta
    48481 ggagaatttg gcctttttgc aacacgatgg tctgacaacg tgcgatgcgc tccatcattt
    48541 cacttatcct ccagtgcatg gatgatggct gctataacac cgtccatatt tttcattact
    48601 tcactgttcc aaaagcggat gattttgtaa ctgatcgagt tcaggtattt tgttctttct
    48661 tcgtcatact cttgctgttc caaatgctga cttccgtcca attcgatgat gagtttcttt
    48721 tcgatgcaga cgaaatcggg
//
LOCUS       DAOQLC010000013        47936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_200_length_47936_cov_25.220046, whole genome shotgun sequence.
ACCESSION   DAOQLC010000013 DAOQLC010000000
VERSION     DAOQLC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_47936_cov_25.220046"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..1014)
                     /locus_tag="PL000_03915"
     CDS             complement(163..1014)
                     /locus_tag="PL000_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNC88070.1"
                     /translation="MNKVTQTYLRFPLARRIEHWVMMLSFTLLGITGLAQKFSTSPTG
                     LSIIFAFGGIESLRSIHHIAAIVMMFGTAWHILVMGYSVFVLRDQMSMLPTLQDAKDG
                     LQALFYNIGFAKTYPQMGRYTFEEKMEYWAFVWGAAVMGITGFIMWNPITATSFLPGE
                     FVPAAKAAHGGEAVLAVLAIIIWHMYGVHIKRFNKAMFTGHQTEAEMLHEHPLELADI
                     KAGITDRRPDAATIRKRQMIYYPIAAILTIAMLGGIYGFINAEETALTTIPPIEETVE
                     APPTVQP"
     gene            complement(1011..2183)
                     /locus_tag="PL000_03920"
     CDS             complement(1011..2183)
                     /locus_tag="PL000_03920"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNC88071.1"
                     /translation="MEDFRRKIHPPQFRTLSLFLGIGLLIFAFTALIIPAQAAPAYQP
                     VHQEEKPGDDFCLACHQEEGIDKTLGNESLPVTINPTQFGLSVHSEEGIACVDCHTDI
                     REYPHPDIKSKSIRDFQITFTEACKDCHEDQYEKAHDSVHQTAFDEGNQNAPLCADCH
                     NPHTQARIIGQESGLLTIAARKEVPSTCATCHTEIYDAYKASAHGNALADGNTDVPTC
                     TDCHGVHSIGDPTTDSFHNNTPALCADCHDNEEVMGKYGLSTEVLETYVSDFHGTTTT
                     LFEQQFPGQQVNTAVCSDCHGVHDIGRVKDSETGIAMQENLLSKCQRCHPDATANFSA
                     AWMGHYQPSPEHYPIVYYVNLFYKFFIPAVLGGMIFFVLTDIYRRIVNRIKGVKHA"
     gene            complement(2378..4552)
                     /locus_tag="PL000_03925"
     CDS             complement(2378..4552)
                     /locus_tag="PL000_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNC88072.1"
                     /translation="MNFKKMLVLLGVMIVVGAILAACGGTPTTAPSPEATEAPATAPL
                     PETPYLADWQGSGHNDVAGEPFRHWDDAAENPDGVPTTCAKCHTSAGYQDYLGLDGSE
                     AGKVDAAVPAAEAQGIQCVTCHNAGTISKTTVVFPSGVEIKAGDDVRCMECHQGRESK
                     VSVDGLIAKFGENVDPDAVPAPVKDDQGKDVVLGFRNVHYYAAAATLYGGMTHGGYEY
                     DGMAYDAKNSHVEGYDSCIGCHDPHTLEVKVDQCAFCHEDVATADDLKNIRMISSASD
                     YDGDGNVEEGMYFEIEGLQEILMAEITKYATGTAGAEIKYDAATYPYFMGADGKAYPN
                     WTPRLLKAAYNYQVSMKDPGAFAHGNKYIVQLLVDSIADLGGDTSKLAREDAGHFAGN
                     TEAFRHWDGEEEGNGTVPFGCVKCHTATGLPTYIKDGGTTVVTGSGSTAITGLSPMPA
                     SNGFLCSTCHNEEAWPERYAIASVTFPSGKTVSYGGKDADGKFVADDSNLCIQCHQGR
                     ESTTSMNNALRGKDLDVVDPAVRFKNIHYFAAGATLFGGEVLGAYQYEGKEYVGMNMH
                     ADPEGKANKCADCHDVHALEPKTESCETCHDTTDPLAVRESDVDYDGDGDVTEGIKGE
                     IDTLAEALYAQMQAYGEANGGAITYDSHAYPYFFGADGKGYATWTPRLVKAAFNYQYV
                     QKDTGAFVHNPKYVIQILIDSIEDLGGNISTYTRPEVPAPAQ"
     gene            4907..5713
                     /locus_tag="PL000_03930"
     CDS             4907..5713
                     /locus_tag="PL000_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HNC88073.1"
                     /translation="MALRGNLRDFTITQLLNLINLASKTGTLILDGPSEQAYVSFRDG
                     KLAYARIGKEDGSLALVLHKANKINVNQYRAIADRAGQMTDKELGLLLINAGYVTQED
                     ILLNLQGYFTEVVRKLFTWVEGIFRFENEMLPPDDRINVRLDLENIIIEGSRQLRELE
                     QLQDEIPSLDMALKFTERPLTNVNLSIEEWKVVKFVDPKNTMRQIASTNKLSDLDIRR
                     IVYGLLQAGLVELVRPQGAVPPPNPKQFPVQNKEEQKSLINKLIGRIRTM"
     gene            5740..6279
                     /locus_tag="PL000_03935"
     CDS             5740..6279
                     /locus_tag="PL000_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HNC88074.1"
                     /translation="MQTVKMVVTGPFSAGKTQFIQSVSEIDVVATERRISSDEERSVK
                     SATTVAMDFGRITVDEDLVLYLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETFR
                     EARSILETFRAYAPTPFVVAANKQDVDDAWDLEDVRHALRLDGDTKLLPCVATNRDTV
                     KTVLLELLYSILKEMDSEK"
     gene            6300..7097
                     /locus_tag="PL000_03940"
     CDS             6300..7097
                     /locus_tag="PL000_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88075.1"
                     /translation="MSSITTDQGIVHYEVYGRGRPVILLHGYQGSWGLWQQTMGYLGA
                     FYRTYALDFWGFGESGTKRETYAVKDFVGLVNQFMEQLGIIRAPLVGHSMGGTVSLLT
                     AMQYPDRVEKVVVIGSPISGSSLYFFPRVFGYRAFGWLTYHNLWIYKSFYRILAPYYS
                     KDKNWAAMMDRDVSRTTLEAFFASIGSLRRTDLRPSLHTIQVPVMGMYGDNDIVVNPN
                     QWKPLQEGVAKSHIERFPKAGHFIMLDEPLEFTQKLKAFLDQENPAS"
     gene            7094..7714
                     /locus_tag="PL000_03945"
     CDS             7094..7714
                     /locus_tag="PL000_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNC88076.1"
                     /translation="MSPQKYYPQKMGRIILQGMEEVMGVNGVETTLHLGSLDHIIQNR
                     PTATSDRTFPFEVVSAILASLEMVYGPHGGRGVALRVGRSTFRYGLKEYGSMLGLTEM
                     AFRLLPLRTKLRTGARVFAELFNKHTDQKVRVEEVENKIYWIIESCPLCWDRKADEAI
                     CHLAVGVLQEALYWLSGGKVFSVEETECIARGNATCTIVIDQIPLS"
     gene            7921..9066
                     /locus_tag="PL000_03950"
     CDS             7921..9066
                     /locus_tag="PL000_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88077.1"
                     /translation="MELKQGERLQPVREQAIVYRDNLFFDKYYIQAFMDEAIRLNRPV
                     RAAFRSDDPAFREHALPLSTSYTPAGSLYLADLWYYPNGPVAGAEPLVIDMQAREIGY
                     YHVPTYMGDRSGDLVFQVPLRAMMAIDSWVHVFVADMVFGQFAHGARFEKRLNEELGF
                     KLKIIGKAIYEGRQVLECSELVKVGKGCVIDPSAVIHGPAIIGDNVTINAGAVIENSI
                     IGNNVNISQDVQVMLSVVGDGAFLPFRAALFMTTIMENSMLAQNTCLQMCVVGRNTFI
                     GAGSTWTDYNLIPAPIRARDGNGKLSLSNRPVMGGCVGHNCRIGSGMIVYPARTIESD
                     VVLAASKERRVIDRDIAFDDSDHHHLKLAHLHQTPYHMQSKGDSESW"
     gene            9094..10182
                     /locus_tag="PL000_03955"
     CDS             9094..10182
                     /locus_tag="PL000_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88078.1"
                     /translation="MDSFAFIIHPIDPKRDVSRKFPFLGRVLTERQIDFFSTFFPPVY
                     ISEIEGITSQSSGKTIKGWFLACPYTPRRMMELPERTVYRKIIQTGRMAEKLGANILG
                     LGAFTSVVGDAGVTIANKLDIPVTTGDSFTVAMAVQAIREAAQVMDIKMESATVAVVG
                     ATGAIGRVCAELLAGEAERVLLVARDEKKLEVLRDRLKVQASSELCISTKMDVLKDAD
                     LILTVTSSIHDVIRPEHLKPGSVVCDVARPRDVSAMVAAVRDDILVIDGGMVDVPGAV
                     DFHFNFGFPDGKAYACMAETIALALEGRFEDYTVGKDITLERVQDITAIAEKHGFRMS
                     GFRSFEREVTIEQIEAVRQNARKGLRRA"
     gene            10195..11100
                     /locus_tag="PL000_03960"
     CDS             10195..11100
                     /locus_tag="PL000_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88079.1"
                     /translation="MGNARLLVVEDDVDIANMLKIYFTSLQYDVDVANRGRDALDKTK
                     QVLPHLIVLDIMLPDIDGYEVCRNLRTSTRTSHIPVIFLTQKDERSDKLQGLELGADD
                     YITKPFDIEELKLRVQGAIRRSERESLTDPRSGLPAGRLIEEQLRRIIREKDWALLDV
                     RVNNFEAFKDVYGFVAGDDVVRFAAMMIGEVVDELGTTSDFIGHAGGDNFIIITTNDA
                     APGIRQKLKDRFANEVQSHYNFIDRQQGFIQAPKAEGGVEKVGFMYLSAGLVSPSLQS
                     FADIREITELAAEARRQDTAATSAA"
     gene            11118..18557
                     /locus_tag="PL000_03965"
     CDS             11118..18557
                     /locus_tag="PL000_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88080.1"
                     /translation="MSSGLGLNLLIAGLIFGALVLVILRVLARTPSAVAPNIAAANSY
                     TIPEFSRSNDNDAIIILAPGGRVEYISALARTFFDLRENEPYDLERLSRRARPSDDFL
                     DLCVSSGYKRLSINGKLVEVASYEVPGTFPVMLISLRSKEFAPALEQSDGASEEILRV
                     VTEFSQSIATSLDLETTAQSILDNVGRLVPSDVLELKLWNSEDQDLDLYRSQYANLGS
                     NGSGRMMTSYFGRLTRQLVESRTPILFGDVRSRPDMNANEEVLPIQSYLGIPLIAGGE
                     LVGSLEAGQTGSGAFGQYDLNLLLLVSGQAAVAIRNARLYVNEQKRASELAGLANLNQ
                     TLGAVRDMQELFTRLVDSVAPLFQAEIIGFLLYDEEKHTMEGKSPFRGLPAHLVDIYR
                     VTVPAESLAEKVITGQQPILTLNAAGDENWRALGLSDIAMAASLRDIALMPLLSSNRM
                     LGYLQIGHHTRGASNFGVEEVRLMSIVANQAAAIIENVLLVQQARGRAQRSDALRRIA
                     SLSGSSATLEEILKFSIRELSNLFQADAGAIFLMDEARGELRLRREATFGLPKDISSS
                     FIQIFMDDPSYRSTVSGSQKPFISGHLSTDRRVLTVYRPLTTTLSLESTIVVPLIVRD
                     RSIGELMLGSFKPDYFNAYDLQVIATAAGQLATAVESSSLLNQTDDSLRRRVDQLSAV
                     TRIGRELGASLDISHLIKTIHEESLHIVQATCSSVFLFRQQGESAGAEIEFSAGCGSR
                     ELSPAETDVLMTREPRIIAEFSQNDQPLPHDGVRSALIVPITYQARLIGLISLHSNKI
                     NAYSASDIELVQILAVQAGISLSNAQRYQSEKRSAELMRRRSDTLVRLTDVSYNLGHD
                     QPLDQALQIIARGIRESTPFRVVLMSMVETETGMLRRLTAVGIPRETMNELMARKQPL
                     ASLQQLMRPEFKISRSYFIPIDLSPVMPSDVHMVTLEVSSSAEKTPNSWDPDDALLIP
                     LENAEGQIVGLISLDDPSNGLRPDQATIESVEVFAAQAALLISNTLRQGELRNRIDSL
                     SAGLQRQQKLIDITQNDLPILLHKDLEQTISLYNLDQRAQRVRAGLAITKSVSRQLDA
                     VSALNALGRETLTQLGMAIALVAENTPDGPRLMHVLGSLPRSTNVESLFGQRNPLRAC
                     LQTGNPILISSLDENDEWRDATLLTSLRAKSVICLPVLVENKTVAAMLAISHEALPDF
                     TDEDSKVYLQISQQASLILQNISLLSQTRRRLDEVNLLLDFSRQLSGMDPDAIVRSLL
                     DSAIRVLQHAHAGVALVWNQQTETLVPRAAAGYADNTTMLAINYRQGEALPGTVFMNR
                     FARRVDEVNFVRDYNLSTENLALYRQATGGRLPVSSLLVPIGTADQNLGLLVLDNFNA
                     TSAFSHEDEALLLSLTQQIALSLDNVRLVQATQERAGQLHALNNAAASLTSSLRSDQL
                     INSLLDQLVPIIQYDTATLWLRDKDRLAVVSARGFPDTEERLGLSVAVQDSALFKEMA
                     QTGQPILVNDVREDSRFMPVDAPRLSWLGIPMISKGEMVGVLAVEKWQAHYYTREQIQ
                     VSLTFASQSAVSLDNARMYEDSVSRATELDQRSQRLATLNRFASALTGLLDTDQILNV
                     TANELLKGLGAKRVSVVTFERGRAYWKISSPRTRIKLPRLLPDSPIFSRLRESLGIFN
                     TDDARNEPDLVPLEEMLGDNPTALLVLPLTSGQNLSALIFAQTSGDARFGFNELEIAK
                     TITNQATVALENARLFQLSVRTAERFAILNETSSLVNSSLNQEDVYLAARKAAERLVP
                     LDSFVITLLDIEKNEIDPVYMIDRGKRISADRVPFGQGMSSQVISTGKPVLISNSEQA
                     NDINTVLAGSSDDEEETRSIVAVPMTLGGKTLGMLSAQSYQEGMYTEEDVQILGTLAN
                     QVVVAIQNGRLFAETQSLASQFEKRVIERTAQLQREQQNTETLLRILTEVSSSLDLDR
                     ALNRTLSLLNEAIGAEQGTIMLLHAEDNLLHYRAGYGYLSDRNDTSGRGFTLRVGEGL
                     AGWVVQNREAVLIDDLHEDPRWVRSSASGMDHRSAIVAPMQVGEDVIGVLMVFQRATN
                     FFSSEMLNLVRAIGGQVAVAINNAHLYELIRDQAERLGVMLRKEQEDASRSQAILEAV
                     ADGVLVTGADNRITFVNTSTERILALDEARLLGNPLDVFGGLFGKAASTWMETIRRWS
                     EDPSSYQSGDSYAEQLELENERIALVHLAPVILQNDFLGTVSIFRDITHEVEVDRLKS
                     EFVATVSHELRTPMTAIKGYVDILTMGAAGALTENQLHFLDIVRNNIERLNILVNDLL
                     DISRIEAGRVTLNPQSVNLREIAEDVIAETLRRSQNENKPMALSLDAPAKMPQVMGDS
                     DRVRQIIGNLVDNAFNYTPSNGTIRVNIHQEKDEVQVDVQDNGVGIALQDQGRIFDRF
                     FRGEHPLVLATPGTGLGLPIVRQLVEMHSGRIWMTSTGVPGEGSTFSFTLPTVHNGV"
     gene            18558..18932
                     /locus_tag="PL000_03970"
     CDS             18558..18932
                     /locus_tag="PL000_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88081.1"
                     /translation="MARILIAEDEPDIRELVAFTLRFAGHEVTATANGEEALQQAGQI
                     IPDIIIMDVRMPRMTGYDACRAMKADPILKDIPVVFLSAKGQDSEIQTGLEAGAEEYL
                     LKPFAPDQLSERVKAILSKFGK"
     gene            18940..19728
                     /locus_tag="PL000_03975"
     CDS             18940..19728
                     /locus_tag="PL000_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88082.1"
                     /translation="MSAIILDGSMVHYEALGRGRPIVFLHDWVGSWRYWVNAMQVAST
                     SFRAYALDLFGFGDTSRDPLRYSLDKQSELVNRFLQEMGIGKVALVGHGLGALVGFTY
                     LKQFPSTVDRMMAVNCPFEYDSVNTRLRTAAMPELVDWLAGRTPEAVSALSDAAKADP
                     QAVSASMASLQGNNYFAAIRDLGVPCLFAYGSEDPAISMPTPDRVDSLPMHMHLVTLE
                     ESSHFPMVDNPTRFNRLMTDFLALDSGASPRELQMKEEWKRRVR"
     gene            complement(19822..20073)
                     /locus_tag="PL000_03980"
     CDS             complement(19822..20073)
                     /locus_tag="PL000_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88083.1"
                     /translation="MEFSMNTGLGGILAFIAGIWVWIKLFQKEGVMKGILGFLCMLYT
                     FIWGLQNMKNEELKLKTPMYVWIAGIVLGIIINAVGSGG"
     gene            complement(20104..20523)
                     /locus_tag="PL000_03985"
     CDS             complement(20104..20523)
                     /locus_tag="PL000_03985"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNC88084.1"
                     /translation="MEHSIKTLWHQPVFSTLLTNRTETTAIIGFGVVHLGLSLAGISF
                     WTCPILAATGVPCPGCGLTRATMQLFHGEVAASLQTHAFAPLFLAALLLMISVLILPE
                     PARKKIISFVNRLETRNGVTAWVFFSLVLYWAFRLIA"
     gene            complement(20578..21624)
                     /locus_tag="PL000_03990"
     CDS             complement(20578..21624)
                     /locus_tag="PL000_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC88085.1"
                     /translation="MAESPKPAHLLIVDDNKVNRMVLSRSLEQQGHIVETAENGREGL
                     DKFRTGTFDLMLLDIEMPEMNGFEVLEACLNDVDLRQIPIIMTSAMEELDAVVKCVEL
                     GAEDYLTKPVNPILLRARVNASLEKKRLRDEQRRLFRTFATKEVAEQLLRSGFSLGGK
                     QVKASIMFADIRSFTSIAENQEPSESIDLLNHYFSLMFDAITNNGGTVNQVVGDGLMA
                     VFGAPIFFEDHSERAVRAALQMLEQLKVFNHNQAAHGKTQIRIGIGIATGQMVAGYTG
                     TQTRAIYTCIGDTVNVASRIEEYTKEALHPILIDNYTRQNLPADILVEDLGKIVFKGK
                     NQAINVFAVMGSSA"
     gene            complement(21630..21962)
                     /locus_tag="PL000_03995"
     CDS             complement(21630..21962)
                     /locus_tag="PL000_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC88086.1"
                     /translation="MSVIDLNTFTNLKETVGADFIGEMIDTFLEDALTQIGQMKSGLA
                     AQDVDLFRRAAHSLKSNAATFGATELAGLARELEMLGREKNLEIGNRLEVLQDAFEQV
                     RGQLLGMR"
     gene            complement(22013..26818)
                     /locus_tag="PL000_04000"
     CDS             complement(22013..26818)
                     /locus_tag="PL000_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88087.1"
                     /translation="MPKTEKAKKTTPKTAKKPTSAASRSGSSERIRKELKQREAELAI
                     IKSVQDGLASKLELQAVYELVGDKIHNIFGAQTTLIASFDLKTETQYFNYYIDRFGRE
                     NLEPTAMSGFTKTIARLKKTFLFNDHVKEQMEAYGAKLVLGPLIPKSALYVPLISGGE
                     VCGVISLQNMDRENSFSPSDVSLLETLASSMSAAMENARLLSETQRLLKETEQHNAEL
                     EAIRKASIDLSSNLEYSTVLDSILKSTSELMPSIENINLFLYENDELVFGTAIANGEI
                     KSTPVSAPRPGGMTDTVAKTGEIMLVEDMRKHPLYKDSPARWTGALIGLPLKFRHRVV
                     GVMNIHFTEARAFANTELRLLQLFADQASIAIENSRLFNESQRLLEESTQRGAELEIV
                     NSVQAKLASQLDGDTLYSLVGEKIAGIFDVQVVMIVSYDAEKNICSFPYLMEMGKRLN
                     QEPLPPSGISGYVLRTHQPVIINANMSNEEERLLGTRSSILGGGEDIKSRMDVPMLVG
                     SQVKGVISLQNVDRENVFKESDLRLLQTLANSMAIALENARLFDETQRRARESSVLNE
                     VGRDISSTLDLKAVMDKIAHHARDLLSASTSAIYLPQGDGSYFKAIAAEGEIAEEIKV
                     DTIYFGQGIIGGLAKTGQGEFINDTAHDPRTIQIPGTPVETEERLMAAPLLTGGKVGG
                     MMAVWRNGGHPFIEADLDFLKRLSLQAAIAIQNASMFDFINKRANELSIINALISGLD
                     PKLDIQTIYDDVGDQIQKIFDAQTVVLTMYDKETNITQFPYIFENGERFYQEPLVLKD
                     GMGGFSGHVIRTGEPIVANRNFDELSQKYNSVLLGANPGDVVVRSGVWVPMSVAGEVR
                     GVISLQNLEHEEAFSEADVRLITTIANSLSVTIENARLFDETQRLLKETKRRASQLEI
                     LNNIAQVLTQQLDVQTIVERVGDKIRELVMEDNVGIGLYDPEARSITAHYVTRKNERI
                     QTPSFPINDSTISASMLGKSLVINRQSPSLWKRLGSNMTALNEVPKSVIMVPMVVGKD
                     LIGGITIQNFERENAYDESIITLMESIASSMATAIQNARLFEDARLARAAAEQANEAK
                     SSFLATMSHEIRTPMNAVIGMSGLLLDTKLDDEQRDYAETIRNSGDALLAIINDILDF
                     SKIEAGRMDIEHHPFDLRDCVESSLDLVSARAVEKGIDLAYIFEGDIPPAIVGDVTRL
                     RQIITNLLSNAVKFTEKGEVVLTVSSQRYRNGGAKEKALLTFSVRDTGIGLSKDGMSR
                     LFQSFSQADSSTTRKYGGTGLGLAISKHLAEMMGGTMWATSDGLGRGSTFIFTVKAEL
                     AEILPAQKRGHSGIQPELQNKHVLIVDDNSTNRYILNAQTSKWGMIPHETASPLTALK
                     WIEQGEAFDVAILDMHMPEMDGLELATKIRAKAKFPLVLFSSLGRREAGDETNVFAAY
                     LTKPIKQSQLFDTLVGLFFRPSAYEEPPTTGRFKPDPQMAVRHPLRILLAEDNAVNQK
                     LALRLLEQMGYRADVASNGIEAIQSIERQQYDVVLMDVQMPEMDGLEATRQIVARWVN
                     KRPFIIGLTANAMQGDREMCLGAGMNHYIPKPIRVVELVDALLKARRVS"
     gene            complement(26990..28183)
                     /locus_tag="PL000_04005"
     CDS             complement(26990..28183)
                     /locus_tag="PL000_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC88088.1"
                     /translation="MEDYKQLIRERILARETFVRATFSGGQKGFSIQWEKVIVRPVEI
                     QGEVQLQFSYFDEKKDTTQNYTFKQAKAKVEDLLALPFRNMFVESNAGNVQVNVSKKG
                     KPIVNEFKPAAVPLTVNLAHNREKHRILSDENARPFLEAVGIMTRDGRIKADMQRKHK
                     QINEFLRLLEETASFSAWSGAPINVVDFGCGSAHLTFAVYYYLRNILNLNARVTGVDV
                     KAELIERHNQTAAAMGWDGLSFQVGYIAEYEPEVKPNVILALHACDTATDDALAKGIQ
                     WESKVIVCAPCCQHELQEQLSHAPTPSQMQPVFHHGILFERMGDILTDTFRATMLRVL
                     GYRTDVTQFVPIEHTGKNLMIRSLKTTQPGDPRWVEDYRNMKSFWQVRPYLEKLLGEA
                     YAQYF"
     gene            complement(28314..28595)
                     /locus_tag="PL000_04010"
     CDS             complement(28314..28595)
                     /locus_tag="PL000_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88089.1"
                     /translation="MQDFPQPTDLGNGLSWTAKFIDWEWARYGRFYFLVDLYQGGTAI
                     KQLKIYTDDYGYGDPNRKQTNAELMERVRATLHAHALSGESNTDAVESG"
     gene            complement(28601..30253)
                     /locus_tag="PL000_04015"
     CDS             complement(28601..30253)
                     /locus_tag="PL000_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC88090.1"
                     /translation="MSTYDVIVIGSGAGGLTAAVALAQAGKKVIVLEQHDRPGGWTHS
                     FTLEGYRFSPGVHYIGSLGEGGGLRRIYDGLGVSQDLTFVELNPDGYDHIFIGEKRID
                     FPKGKENLIERLKSHFPHEAKGIDSYFTDLTNMMEGLRRIGNLSNPLETAKSAGNILK
                     WIRSTGADLINAHISDPVLRGVLAAQSGDHGMPPSQVSAFIHAGITHHYLDGGFYPLG
                     GAFTIPRAFVRALKKSGGEIRLQCRVKRILLEGKKVLGVELDSGEEIRADVVISNADP
                     EVTFGKLIGKENLSARLKRKIDSVQYSTSCLSLFFATDMDLRRDAGLDSGNMWYYDHA
                     DVDKIYTQGLTDAVLNDETPPGMFLTTTTLKDPSKMHHGQHTCESFAFVGYDAFEKWA
                     NSKYGARPADYEAMKEDLAWRMVRGLEKRIPGLSKHITFYSLGTPLTNEHYLNATRGN
                     LYGIDKRPSQVGPLGFTPRTEFEGLYLCGQSTISHGVAGVTQSGIDAAKAVLNCRARD
                     ILTHTGTSPVFLQSENVSAWPEHLKKKMERGEAAKEEEEREV"
     assembly_gap    30368..30467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30474..31349)
                     /locus_tag="PL000_04020"
     CDS             complement(30474..31349)
                     /locus_tag="PL000_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88091.1"
                     /translation="MITIENVTKRYDNTTVVHELNLQIHPGEIVGIIGHNGAGKSTTM
                     KMIAGLIEPTSGVVRVMGEDIQKGNARIKQKIGYLPEESPLYEAMTAQQYLLFFSELY
                     QMPRQKALKRIDDLLNSLGLHETKKLTGEFSKGMKRKTAIARALLHDPDLLILDEPNS
                     GLDPLTSFFIINYLKTLRREGKTILLSAHNLFHVETICDRVGIIKNGKLLIFDTMEAI
                     RARLGKREYQVIFHADQKLDYEEVNGNYIFRTAEVDGIAHMLETVSANKWTLMDLSMR
                     ESALEEIYVKLMTNE"
     gene            31478..33103
                     /locus_tag="PL000_04025"
     CDS             31478..33103
                     /locus_tag="PL000_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC88092.1"
                     /translation="MNQILVIAWREITRLRKRFGGGASPLAVLALLAVLGGSSYFLRD
                     AVSLGSGLYRVGVMGNVPTINDSRFAVMEVNEEQGRVLLEQKAIDLLIIDSQVIARED
                     DKSQFAVRALKQYMEAEELNRVGNSFSEADAFPLRVAINYLGAEQAPVQGEAGGTLPT
                     VKPEEVIIPSLTPPPAPFTQVLVALLYILPITFISIFFTSSFMDEKMNRRLTILLSAP
                     VSPFQIIFGKMLPYAIFALVSTALIAALTKTNIPLALAIFAPTTLFIFAIYLMVPLFY
                     RTFKDTTFIAMLVTTLTTAYLVFPAMFTNVNDLAYISPLTLAVKMYRGEPFGWREYLF
                     PSLPMAAIFGFAMFAGTRMLNEEFLMGYKPLTRKIKDAIYLMMAHAKPSIAVPLWSLI
                     VIPAVYMTQIVFLAFATNLPVGLILGFTLIASALVEEVVKTIGIVVLAERGVVKTWRE
                     IIGLSFLSALGFLIGEKLLLLVSISMVSEAQVSGALFGAGLLLFVPLAAHFVFVTIVT
                     VMAAKWRVPYWLALGVGTILHFIYNVNVMRGGM"
     gene            33112..34134
                     /locus_tag="PL000_04030"
     CDS             33112..34134
                     /locus_tag="PL000_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88093.1"
                     /translation="MLAVAKKELMSVLRDRTIVISILIQLFIASFSSALLLGMLSLYD
                     SDTIMRFSGAGMRIGFVRAPVNVLEQLMGERGLTIVQFPSLGEAQAAYYRGEVLAIVE
                     PPQDANGLTETRIYLPESDTVNSLIRMVIQEPLKQYENHLRGQKGIEVRYADLKGKPS
                     TSYEFVYSVLIPMLMFFPAFVAGSMAVDSLTEEVENKTLQTLLSAPLSVNGMVAGKIL
                     ASVILSVAQCMAWLTLLQLNGIVIQNAGWILTLATIVAGITSTVAALGAVMLQDRERS
                     QFIYSLMLLAAAGISTLLNISPITTLSRLAIGDAYTNGWNVTVFAVFLAGLYFLLIRM
                     SRRLLA"
     gene            complement(34144..35148)
                     /locus_tag="PL000_04035"
     CDS             complement(34144..35148)
                     /locus_tag="PL000_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC88094.1"
                     /translation="MSLQLWHNPEWQKQAHDWIRSEAGKNSIQLTGEIEQPHAYAWST
                     VMRVSSSAGMLFFKATAAETVYEIALTKKMAEWFPDSLPDIISADPARGWMLMRDGGE
                     QLRASIRPTKNIKPWEPVITRYADLQIGLASHVNEVLALGIPDHRLGTLPTLYKQLLK
                     DEDSLMIGKEKGLTETEFQQLKELQPRFENICSDLAAIQIPESVNHGDFHDGNILLKD
                     GRITFFDWGDASVTHPFVSLRTFFVSIEIALDLEDYSFTPAMAALLELYLKPWQAFAS
                     PASLQTAYRLSKPVASIVKTLSWHTTISRLEGSKRDEYAWIVPEVLREFMVYEKALQG
                     "
     gene            complement(35152..35889)
                     /locus_tag="PL000_04040"
     CDS             complement(35152..35889)
                     /locus_tag="PL000_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010081311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC88095.1"
                     /translation="MQILGIDVGGSGIKGAPVDIETGQLAAERLRIKTPKKGEPEPIA
                     EVVKQIAQSFNWSGPIGIGFPAPIKSGVAMMAANVSDKWVGLNADELFSKVTECECTM
                     INDADAAGLAEMTIGAGRGQSGTVIMITLGTGIGTAIFHRGHLLPNTEFGHLEIKGKD
                     AEFRASAFARERDKLSWKKYAKRLNRYLSTMEKLFWPDLFIVGGGISKDSKKFLPLLT
                     IETPVVPAQLLNDAGIVGAAMGAAQKK"
     gene            complement(35984..37072)
                     /locus_tag="PL000_04045"
     CDS             complement(35984..37072)
                     /locus_tag="PL000_04045"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC88096.1"
                     /translation="MQPSAIPTEKLLEFMEAAGVPGLAFSVIQNRQIISTQVIGIKNA
                     EIKDDPINEDTIFQAASLSKPVFTYGVLTLQQEGKLDLDKPLHDYLPLPEANEIPQLK
                     LITARQALTHTSGLQNWRFDIEDKFEFEFEPGTGFSYSGEGFFYVQRVIEQITGQSIE
                     SFLQDRVLRPFGMSNSTYIWRAEHEAMISMGHRDRGQKVNPWNAWQGRKLMEIAAQKN
                     KALDDWLYQDFVEALPQVHANLSPLPNNMIPNVAGSLLTTAPEYARFMLRLLEPKNEI
                     AEQMLKSQPPLNSPLSWGIGIGLEKIGDRNCFWHWGENGCFENFMFGDPETGNGIVIL
                     TNGNRGLKICERIVQAVTGQSLNAFLWV"
     gene            complement(37112..38167)
                     /locus_tag="PL000_04050"
     CDS             complement(37112..38167)
                     /locus_tag="PL000_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HNC88097.1"
                     /translation="MENRKRARELGITPGILPTGKLNAITDVAGVKVGQVTLIEGTDI
                     RTGVTAILPHAGNLFQEKVPAGVAVGNGFGKLMGSTQIMELGEIETPIILTNTLSVPR
                     AAEALILFTLGQAGNGEVRSVNPIVGETNDGTLNNIRRLAITTEHVIQAIESAKDGQV
                     FEGNVGAGTGTICFGWKGGMGTSSRVLPQNLGGHTVGVLVQSNFGGVLQMDGIPVGKN
                     LGQYYLKDKLNDSSADGSIMIVVATDAPLSDRNLSRLARRGLSGLAKTGAAMSDGSGD
                     YVIAFSTAEEVRRTAERRGKIWAYPEVPNAGMSALFQAVIEATEEAIYNSLCMAETMT
                     GHEGRTIHALPLSKLVF"
     gene            complement(38169..38660)
                     /locus_tag="PL000_04055"
     CDS             complement(38169..38660)
                     /locus_tag="PL000_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88098.1"
                     /translation="MSKLKPVALMALLVFVLACQTLQPQPPTATAIPTPPPDLTVNTD
                     EEFVLVAGQSATINDSPLTLKLVGVTDDARCPSQIECAMSGPVSLTLIVTTDQGGRHE
                     VNLQTFTNAQGRAPEMEFEGIQSHVEIEGYSIRVMAVLPYPATLADRGKGVYQITLIV
                     KKV"
     gene            38829..39698
                     /locus_tag="PL000_04060"
     CDS             38829..39698
                     /locus_tag="PL000_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88099.1"
                     /translation="MQLNIQHLGKQYKRDFWGLKDFSLEIKPGILGLLGPNGAGKSTF
                     MRMLATITKPTEGSIKWNEVDVVKQPDALRDVLGYLPQDFGVYPNLTAVEFLEYMAAI
                     KGLDAVTAKKRIDELLQLTNLVQAAKRPLGGYSGGMKQRVGIAQALLNDPQLLIVDEP
                     TVGLDPEERVRFRNLLSDLSGERIVILSTHIVSDVEATATHIALVNKGQLLREAAPEK
                     LLQELEGKVWEWTIHSDDLPALKQKHIISGTIRRSDGVQVRVVSSTQPTAESRNASPN
                     LEDAYLFFIGGKQ"
     gene            39695..41257
                     /locus_tag="PL000_04065"
     CDS             39695..41257
                     /locus_tag="PL000_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC88100.1"
                     /translation="MKSARIIYQLARADFLERVRRYSFLVMLGLVVFLGYQTAIGNMS
                     LELGAYRGEFNSAWVGAMMSLIGTFFIGWFGFYLVKGSVARDRETGVGQIMATTPLTR
                     PLYLLGKWLSNFAVLMSMVGVLALAGIAIQLLQGENTQIQLLEFLDPFVFIVMPLMAL
                     VAALAVLFESISFLQGGFGNIVYFFAFIMFLPLFMENDTLQKYPAFEPMGLGLLAGEM
                     GDAVRAIHPDYDGSFTLGGGIVEQATTTFVWNGIAWTPEYLFARFALIFFAVGLILFA
                     ALFFDRFDPSRSKPRKARSASSSEPTPASTPQAFPAPHLTPLNKSANRFSFFTVLIAE
                     LKLLIKGQRWWWYAISLGIIVACFVSPSKVVREIALPIAWIWPILIWSSIGNREVYNN
                     VQQLTFSSASPLWRQVPAQWLAGWIVTLLVSIGAISRFLMDGDTVGLLALLSGTIFIP
                     SLALASGVWSGTSKLFEILYMVIWYLGPLNNAPGLDFIGSHGNGRPEFFIPLSIALVA
                     FAFFGRARQLQQ"
     gene            complement(41267..41785)
                     /locus_tag="PL000_04070"
     CDS             complement(41267..41785)
                     /locus_tag="PL000_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88101.1"
                     /translation="MHTCPNCTNELSNIGAGAQFVQCAKCASILELADTVTLLTDRPV
                     STPIQWAAHCVLPDGTRFVSDGQILLAASYTGTAEPLPGKALPVETFQRLLNWNVKSN
                     FGIEHLLEDPARGNYYAPFDVLLNGKYIKYLTSLRYKQKLSFHVNAPLEPIVLVDGGK
                     KIGVLMPMKPPK"
     gene            complement(41834..43003)
                     /locus_tag="PL000_04075"
     CDS             complement(41834..43003)
                     /locus_tag="PL000_04075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC88102.1"
                     /translation="MTQTYDAIVIGAGVMGASIAFHLAERGLKTAILERKVTASGATG
                     HSSGLVRMHYDLAAESELTFTSYKNYFANWRERVGGECGFQKTGFLQVAKREHEDKLR
                     GNVANQQKIGINTSVISADEVKKLFPDLVTEHFDFAAYEPDSGYADATLTTNSFLDSA
                     KRNGAALIQNCEVTAIHTAGGKMTGVSTTKGDFAAPIIVNAAGPWAKQVAALAGVDVP
                     LVTWTHDVAFLHRPPSLGKFPAVIDDVINCYFRPEGSALILAAGEDESLRGEAPDAED
                     QTPTPTFVEKLIDAMVRRMPKIEESGLHSIHVGRDGITPDQRAIYSAAGPDGFYLACG
                     LSGTGFKTSPAAGASMVELILDGKPKTVDITPFRFQRFAEGKLIEGEYGYGHIWK"
     gene            43138..43527
                     /locus_tag="PL000_04080"
     CDS             43138..43527
                     /locus_tag="PL000_04080"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC88103.1"
                     /translation="MKKQSISQISEQIAAPLAAISTPQRIAILLAIGTGEACVCHLET
                     ALGWRQAYISQHLMALRKADILQDRREGRYVFYKLRNASFLDLITASATLSGLAAEAV
                     STLVNTRTYPACECPQCVPALIPANSL"
     gene            43535..44575
                     /locus_tag="PL000_04085"
     CDS             43535..44575
                     /locus_tag="PL000_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNC88104.1"
                     /translation="MTTLSSPSQSHKYLPSLVFFSAVIWLIAYNTIQPLADWISYTAL
                     GLARDSRLGESLAFFLYDVPKILLLLSGMVFLISIVRTFFSPERTRALLGGKREGVGN
                     IFAAMLGIVTPFCSCSAVPLFIGFVESGIPLGVTFSFLIAAPTINEVAVILLFGLFGW
                     KVAGLYVASGLVVAIVAGFIIGRLKMERYVADFVWKVQRKVDAFEEKLTWEDRIERAW
                     DSVKEIVGKVWLYVVVGIAIGAGIHGYVPEDAMASILGKQAWWSVPVAVLIGIPLYSN
                     AAGIVPIVGTLIEKGASVGTALAFMMSVIGLSLPEAVILKRVLKMPLLVTFFAVIAVA
                     IMITGYMFNLIL"
     gene            44632..44883
                     /locus_tag="PL000_04090"
     CDS             44632..44883
                     /locus_tag="PL000_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC88105.1"
                     /translation="MLTIKILGSGCENCKRLAWLADRVVNHMGVQAQIVKVTDYAEIM
                     QYPIMSTPGLVINEKLVSSGRIPSETEISVFLTTALETA"
     gene            44915..45988
                     /gene="arsB"
                     /locus_tag="PL000_04095"
     CDS             44915..45988
                     /gene="arsB"
                     /locus_tag="PL000_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNC88106.1"
                     /translation="MKNETRKELSFLDRYLTLWIFLAMAVGVGIGFLFPKAVADFNAA
                     VSIGTTNIPIAIGLILMMYPPLAKVRYDELGDVFRNWKILSLSLVQNWVIGPVLMFVL
                     AIVFLRGYPEYMTGLILIGLARCIAMVIVWNELAKGDNEYAAGLVAFNSIFQVLFYSL
                     YAYVFITILPTWFGMTGNIVNITIGEIARSVFIYLGIPFIAGFFTRFFLVRAKGKDWY
                     HKVFIPKISPLTLVALLFTIVVMFSLKGDIIVQLPLDVVRIAIPLLIYFVIMFLVSFF
                     MGKSIGADYSRTTTLSFTAASNNFELAIAVAVAVFGINSGAAFAAVIGPLIEVPVMIA
                     LVNVAFFFQKRYFGGVVSSAPAR"
     gene            complement(46047..47186)
                     /locus_tag="PL000_04100"
     CDS             complement(46047..47186)
                     /locus_tag="PL000_04100"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC88107.1"
                     /translation="MNNRSKELDGLRGIAILLVIAHHVLKRADYFTANGFLHFITRLS
                     LVGWMGVDIFFVLSGFLITSILLKTREEKNYYKNFYARRVLRIFPLYYTALALFLIAV
                     KFVPAVAETADSFIPYLPTFLLYQQNWLYLFAIYPSAYLSVTWSLAIEEQFYLIWPAI
                     VRIVNKHTLTWISAGIVILSLLIRTAAILLSPDSEQLPVVFFYNTFTRFEGLALGALT
                     AIFFVTPTNWQETAKRFALPVSIITMILFLYLAFIASPAGPHPLYRNIPLGTIGYTLL
                     AFSSAAMIVFLTSGAETSLIRKFFANGGLVFFGKYSYSMYLFHMPVALTLLDIMVKAK
                     VRGWEYYAAYSLLTVAITTVLALLTWNILEDPLLKLKRHFTNEPT"
     gene            complement(47183..47806)
                     /locus_tag="PL000_04105"
     CDS             complement(47183..47806)
                     /locus_tag="PL000_04105"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC88108.1"
                     /translation="MTQPTPSAKQYSSLEELFSAMKRMNDDPLAKAGTNIVISRGNPK
                     ARLLIIGEAPGPEENIQGKPFVGRAGQMLDKILQAADFDPEKDVYITNSVFRMPPGEE
                     GKTFRKPTDAETDFYRPYVFEIIRLIDPLVMLLTGNVASYSILRKTGITSLRGKWTES
                     QGRWIMPIFHPSYLLRNPSKEPGSPKAQMWEDIREVRKKYDELIAAS"
BASE COUNT        10803 a        11917 c        12640 g        12476 t
ORIGIN      
        1 tgaaatacct gcgggtggtg gatcaccgcc tgaccagtga agagtatgaa actgtatcag
       61 tgaaagtagg ggaagccatt actggagatt aagagtcaac caatgcacaa caatcaaaaa
      121 gagccatccc gaaaggatgg ctctttttga ttgtttccaa aattaaggtt gtactgttgg
      181 cggggcttca accgtctctt cgatgggcgg gatggttgtc aatgcagttt cttcggcgtt
      241 gataaaaccg tagattccac cgagcatggc gatggttaga atggcagcaa tggggtagta
      301 aatcatctga cgcttacgga tggtggccgc gtcgggtctt cgatctgtga tgcctgcctt
      361 gatgtcggca agttcgagcg ggtgttcgtg gagcatctca gcctcagttt ggtgtccggt
      421 gaacatggcc ttgttgaaac gtttgatgtg tacaccatac atgtgccaga taatgatggc
      481 caacacggca aggacagctt cgccgccatg tgcagctttt gcggcaggca cgaattcacc
      541 cggcaggaaa gaggttgctg taattgggtt ccacatgatg aaaccggtga tgcccatcac
      601 agccgcgccc cacacaaagg cccagtattc cattttttct tcgaaggtgt agcgccccat
      661 ctgtggatag gttttggcaa aaccaatatt ataaaaaagc gcctgcaaac catcttttgc
      721 atcctgcaaa gtaggcaaca tggacatttg atctctgaga acgaagacgc tataccccat
      781 gacaaggatg tgccaggcgg ttccgaacat catcacaatg gcggcgatat ggtgaatgga
      841 gcgcagtgat tcgataccgc caaaagcaaa gatgatggaa agcccggtgg ggctggtcga
      901 aaacttttgc gcaagccctg tgatgcccaa cagggtgaag gagagcatca tgacccagtg
      961 ctcgattcgg cgtgctagtg gaaagcgcaa ataggtttga gttactttgt tcatgcgtgc
     1021 tttactcctt tgatgcggtt gacaatacgt ctgtaaatat ctgtgaggac gaaaaagatc
     1081 atgccgccaa gcacagcagg gataaagaat ttatagaaga ggttgacgta gtaaacgatt
     1141 ggatagtgtt ccgggctggg ctggtaatgt cccatccatg cggcagagaa gttggccgtg
     1201 gcatcgggat gacagcgctg acatttggag agcaggtttt cctgcatggc gatgccagtt
     1261 tcagaatctt taactctgcc aatatcatgc acgccatgac aatcactaca aacggctgtg
     1321 tttacctgtt gtccggggaa ttgctgttca aagagggtgg ttgtagtgcc gtgaaaatct
     1381 gaaacatagg tttcaagcac ttcggtcgaa agaccatatt tacccataac ttcttcatta
     1441 tcgtggcagt cagcgcagag ggcgggagtg ttgttgtgga aagaatctgt ggttgggtca
     1501 ccgatgctgt gaacgccatg acaatcggta caggtgggca catcggtatt accatcagcg
     1561 agggcatttc cgtgggcgct ggctttgtaa gcatcgtata tttcagtatg acatgtggca
     1621 caggtggaag gcacttcctt gcgggcggca atggtaagca ggccgctttc ctggccgatg
     1681 atgcgggctt gggtgtgtgg gttgtgacag tcagcacata ggggggcgtt ttgattgcct
     1741 tcatcaaatg cggtttgatg aacactgtcg tgtgcttttt cgtattgatc ttcgtggcag
     1801 tctttgcagg cttcagtgaa ggttatttga aaatcgcgga tggatttgga tttaatatct
     1861 ggatggggat attctctgat atcagtatgg caatcaacac aggcgattcc ttcctcgctg
     1921 tgaactgaca gcccaaattg agtaggattg atagtgacgg gcagggattc gttaccgagg
     1981 gttttgtcaa ttccctcttc ctgatggcag gcaaggcaga agtcgtcccc aggtttctcc
     2041 tcttgatgca ctggctggta tgctggtgca gcttgagcag ggattatgag tgctgtaaag
     2101 gcgaaaatta aaagcccaat tccaaggaac aggctgaggg tccggaattg aggcgggtgg
     2161 atttttcttc taaaatcttc cattgcattc tcctattaaa ggatattttg tccaattata
     2221 cgcccgataa aaagtgtcat ggggcgattg ccccatattg tacgagaggt tatcactggg
     2281 atatagtgct gaatatcact gttttggtgt gattaacaag ggattaaaaa gagatggcgg
     2341 acatttctgt ccgccatcct tttttgttgg aattatctta ctgagccggg gctggaactt
     2401 ccgggcgggt gtaggtgctg atgttgccac caaggtcctc aatggagtct atgagaattt
     2461 ggatgacata tttcggattg tgtacgaatg caccagtgtc cttttgaaca tattgatagt
     2521 tgaaggcagc ctttaccaga cggggtgtcc aggtggcgta gcccttacca tcagcgccga
     2581 agaagtaggg gtaggcatgg ctatcgtagg tgattgcacc accgttggcc tcgccataag
     2641 cctgcatttg agcatagaga gcttcagcaa gcgtgtcgat ttcgcccttg atgccttctg
     2701 ttacatcacc atcaccatca tagtccacat cactttcgcg gacagccaat gggtcggtgg
     2761 tatcgtggca ggtttcgcaa gactcagtct tgggttcaag tgcatgaaca tcatgacagt
     2821 cagcgcactt gttggccttg ccttcggggt cggcgtgcat gttcataccc acatactctt
     2881 tgccttcata ctggtaggca ccgagtactt ctccgccaaa gagggttgcg ccagcggcga
     2941 agtagtggat gttcttgaag cgcacagcag ggtctaccac atcaaggtct ttgccacgca
     3001 gggcattgtt catgctggtg gtggattcgc ggccttgatg gcactgaata cataggttgg
     3061 agtcatcggc tacaaacttg ccatcggcat ctttgccacc ataagagacg gtcttgccgc
     3121 tcgggaaggt gacagaagcg attgcatagc gttcgggcca ggcttcttca ttatgacagg
     3181 tggagcacag gaaaccgttg ctggcaggca tggggctgag gcctgtgatt gcagtagagc
     3241 cacttcctgt aactacagtg gttccgccat ctttgatgta ggtgggcaga ccagtggcgg
     3301 tgtggcattt cacacagccg aatggaacgg tgccgttacc ttcttcttcg ccatcccagt
     3361 gacggaaggc ttcggtgttt cctgcaaagt gaccagcatc ttcgcgggcg agtttgctgg
     3421 tgtcaccgcc gaggtcggca atggagtcaa caagcagttg aacgatgtat ttattgccgt
     3481 gagcaaacgc accaggatcc ttcatggata cctgatagtt gtaggcggct ttgagcaagc
     3541 ggggagtcca gttggggtag gctttgccat cggcacccat gaagtatggg taggtggctg
     3601 catcatactt gatctcagca ccagcagtgc ctgtggcata cttggtgatc tcggccatga
     3661 ggatttcctg caggccttcg atttcaaaat acatgccttc ttcaacgttt ccgtcgccgt
     3721 cgtagtcaga ggcggaagag atcatgcgaa tgttcttgag atcatccgcg gtagctacat
     3781 cttcatgaca gaaggcgcac tggtccacct taacttcgag tgtgtggggg tcgtgacaac
     3841 cgatgcagga atcgtaacct tcaacatggg agttcttggc atcgtaagcc atgccgtcat
     3901 attcataacc gccgtgagtc ataccaccat aaagggtggc ggctgcggca tagtagtgaa
     3961 cattgcggaa accaagcacg acatccttgc cctggtcatc ctttacagga gcaggaacag
     4021 catcggggtc aacattttca ccgaatttgg cgataagacc atcaacactg accttggatt
     4081 cgcgtccctg atgacattcc atacagcgga catcatcacc ggctttgatt tctacgccgg
     4141 aggggaatac tacggttgtc ttggaaatgg ttccagcgtt gtgacaagtc acgcactgaa
     4201 taccttgagc ttcagcagcg ggaacagctg catccacttt tccagcttca gagccgtcga
     4261 ggccgaggta atcctggtag ccagcgctgg tatggcactt ggcacaggtg gtgggcacac
     4321 catcaggatt ttcggcagca tcatcccagt gacggaatgg ttcgccggct acgtcattat
     4381 ggcctgagcc ctgccaatcg gccagatagg gggtctctgg caatggggca gtggccgggg
     4441 cttcggttgc ttccggggat ggagcggtgg tgggggtgcc gccgcaggcc gctaaaatag
     4501 cgccgacaac aatcatcaca ccgagcagta ctaacatttt cttgaagttc attttgcatt
     4561 ctcctttatc aaagttttgg aaaacatcta atatttctcg tttatttctg tgccgcctcc
     4621 tttagtgaaa taatgctcaa tctattcttg ggcatttcga gcaaatacct ccgaattcta
     4681 tgttttttga agattaaaaa caagttaatt tgcgccatca tgcacgtgac taatgtcaca
     4741 tatagggctt ttgtaattgc ctaaaattgg cgaggatgtg aataaaacag ttattcagac
     4801 gttgtttcca ccctcacaag aatgataagc aggttaattg acgcaatgcc accttgcatg
     4861 tataattttc atctagtcat ccatggatgg cgaatataga ggatatatgg ccctgcgtgg
     4921 aaatttacgt gattttacaa tcacccaatt acttaatttg atcaacctcg ccagcaaaac
     4981 cgggacttta attttggacg gtccttcgga gcaggcatat gtttcctttc gtgatgggaa
     5041 gctagcttac gcccgtatcg gcaaggaaga tggcagcctt gcgttagtgt tgcataaggc
     5101 gaacaagatc aatgtaaacc aataccgtgc aattgcggat cgtgcaggcc agatgaccga
     5161 taaggaactc ggcctgcttc ttattaatgc cgggtatgta acacaggaag atatcctgct
     5221 taatctgcag gggtatttca ccgaagttgt aagaaaactc ttcacatggg tggagggaat
     5281 cttccgtttt gaaaatgaaa tgcttccccc ggatgaccgc atcaatgtgc gccttgacct
     5341 tgagaatatt attattgaag gatcgcgcca actccgtgag ttggagcagt tgcaggatga
     5401 aattccgagc cttgatatgg ctttgaaatt tacagagcgt ccgcttacca atgtcaacct
     5461 cagcattgag gaatggaaag tggttaagtt tgtggacccg aaaaatacca tgcgccagat
     5521 cgcaagcacg aacaagttga gcgatcttga tatccgcagg attgtgtatg ggttgctgca
     5581 ggcaggcctt gtagagttgg tacgtccaca aggtgcggtt ccgccgccta acccgaagca
     5641 attcccggtt cagaataaag aagaacagaa atcgttgatc aataaattga ttggccgcat
     5701 tcgaacaatg taatcatcag ggctagaagg atagaaatta tgcaaacggt caaaatggta
     5761 gtgacaggtc cattcagtgc tggcaagacg cagttcattc agtctgtcag tgaaatagat
     5821 gtggttgcaa cggaacgcag gatttcttct gacgaagagc gttctgtcaa gtctgctact
     5881 acagtggcga tggatttcgg gcgtatcact gtggacgaag acttggtcct gtacttgttt
     5941 ggtacacccg ggcaaaaacg cttcgacttt atgtgggaga ttctttcaga aggaatgctg
     6001 ggtttcatcg ttatggtgga tagcacccgc cctgaaactt tccgtgaggc tcgctcgatt
     6061 ctggaaacct tccgcgcgta cgccccgacc ccttttgtcg ttgctgccaa taagcaggat
     6121 gtggacgatg cctgggatct tgaagatgtg cgccatgctt tgcgtcttga tggcgataca
     6181 aagttactac cctgtgtggc aacgaaccgc gataccgtca aaacggtttt gctggagttg
     6241 ttatatagca tcttaaaaga aatggactcc gaaaaatagg gtcaggttgt atccttgctg
     6301 tgtcttcaat tactactgat cagggaatag ttcattatga agtatacggg cgggggcgcc
     6361 cggtaatttt gctgcatggc tatcaaggct catggggatt gtggcagcaa accatggggt
     6421 atttgggcgc tttttatcgc acttatgcct tggatttttg gggatttggc gaatctggta
     6481 caaaacgtga gacatacgct gtaaaagatt ttgttggctt ggtcaaccaa ttcatggagc
     6541 aattgggaat tatccgggct ccattggttg ggcatagcat gggtggtact gtcagtttat
     6601 tgacggccat gcaatacccg gaccgggttg aaaaggttgt ggtaattggc tcacctattt
     6661 caggttcctc tttgtatttt ttcccgcgcg tgttcggata tcgtgcattt ggctggctga
     6721 cctaccataa tctttggatc tacaagtctt tttaccgcat ccttgcacca tattattcca
     6781 aggataagaa ttgggctgcc atgatggacc gggatgtatc tcgaacaacg cttgaagcct
     6841 tttttgcaag cattggctcg ctcagaagaa cggatttacg ccccagcctc cataccattc
     6901 aagtccctgt aatgggtatg tatggagaca atgatattgt ggtaaaccca aatcaatgga
     6961 agccattgca agaaggtgtt gcaaagtctc atattgagcg tttcccaaaa gccgggcatt
     7021 tcatcatgct ggatgagccg ctagaattca cccaaaaact aaaagcattc ctagaccaag
     7081 agaatcccgc ttcatgagtc cgcagaaata ctatcctcag aaaatgggga gaatcatcct
     7141 gcaaggcatg gaagaagtaa tgggtgtgaa cggggtggaa acaacccttc atcttggttc
     7201 ccttgaccac attatccaaa acaggccaac agctacgtcg gaccgcactt ttccttttga
     7261 ggtggtcagt gccattttag cgtctcttga aatggtttac ggtccacatg gtggaagggg
     7321 agtggcgctg cgtgtaggcc gatccacttt ccgatatgga ttaaaggaat acgggtcaat
     7381 gcttggattg accgagatgg catttcgcct tttgcctctt cgcaccaaac tgcgcacagg
     7441 tgcgagagtg tttgctgagc ttttcaacaa gcacaccgac caaaaagtgc gcgtggaaga
     7501 agtagagaat aagatctatt ggatcatcga aagctgtccg ctttgttggg atagaaaagc
     7561 ggatgaagca atttgtcatc ttgcagtcgg cgttttgcag gaggctttgt attggttaag
     7621 tgggggtaaa gtgtttagtg tggaagaaac agagtgtatt gcacgaggca atgcgacctg
     7681 taccattgtg atcgaccaaa tcccgctttc ataacaaaat aatatctgat tttaacagtt
     7741 ttgtttgacg ggcattttgc ctcatacttt gatttataat catacattat gcttaaagtt
     7801 gtcctgcatg cacccaatcc aatcatgccg ttttgtgaac cagcacgcga attgcgtatc
     7861 caaaactcgc ctctttggtt gcaccagcgc aacttattgg ctccatatgt aacgcgtgaa
     7921 atggaactta aacaagggga acgtttacag cccgttcgtg aacaagccat agtttaccgg
     7981 gataacctct tctttgataa atactatatc caggctttca tggatgaagc catccgtctc
     8041 aacagacctg tgcgagctgc tttccgctcc gacgaccctg ctttccgtga acatgccctg
     8101 ccgctttcca cttcatacac ccctgcagga agtttgtatc ttgcggatct gtggtattac
     8161 cctaatggcc cggtagccgg ggcagagccg cttgtcattg acatgcaggc acgggaaatt
     8221 gggtattacc atgtaccaac ctacatggga gaccgcagtg gcgaccttgt ttttcaggtg
     8281 cctttgcgtg ccatgatggc cattgattca tgggtgcatg tttttgttgc agatatggta
     8341 tttggtcagt ttgcccacgg tgcccgtttt gagaagcgcc taaacgaaga acttggattt
     8401 aagctgaaga tcattggtaa agccatttat gagggcaggc aggtcctcga atgttcagag
     8461 ttggtaaagg tgggtaaggg gtgcgtcatt gatcccagcg ccgttattca tggaccggct
     8521 atcattggcg ataatgtaac catcaatgcc ggtgcggtca ttgaaaacag tatcattgga
     8581 aacaatgtca atatctcaca ggatgtacag gtaatgcttt cagtggttgg agatggggcc
     8641 ttcctgccct ttcgtgcggc attgttcatg accaccatta tggaaaacag catgttggca
     8701 caaaatacat gcttgcaaat gtgtgttgtt ggcagaaata cattcattgg cgcgggttct
     8761 acatggacag attacaacct tatccctgcc cccattcgtg cccgtgatgg taacggaaaa
     8821 ttaagcctgt ccaaccggcc tgtgatgggt ggttgtgtag gccataactg ccgtatcggt
     8881 tcggggatga tcgtttatcc ggcccgtacc attgaatcgg atgttgtgtt ggcggcttcc
     8941 aaggaacgcc gtgtcattga ccgggatatc gcgttcgacg atagcgatca ccaccatctt
     9001 aaacttgccc atcttcacca aaccccatac catatgcaat caaaaggtga ctcagagtcg
     9061 tggtaaaagc cgaccgagca tcatcgagac aaaatggata gttttgcgtt catcattcac
     9121 cccattgacc ccaagcgcga tgtcagccga aaattccctt ttctaggccg agtattgact
     9181 gagaggcaga tcgacttctt ttccaccttt tttccgcctg tttacatctc agagatcgag
     9241 gggatcacct ctcaatccag cggaaaaacc attaaaggct ggttccttgc atgtccgtat
     9301 actccaagac gcatgatgga gttgcctgag cgcacagtct atcgtaaaat tatccaaaca
     9361 ggccgaatgg cagaaaaact tggagccaat attctcggcc ttggtgcctt cacttctgtg
     9421 gtgggggatg cgggtgtgac cattgcaaac aagctggata tccccgtaac cacaggcgat
     9481 tcctttactg tagccatggc ggtgcaagcc attcgggaag ccgcacaagt gatggatatt
     9541 aaaatggaat cagccactgt ggcagtggtt ggtgcgacgg gtgcaattgg ccgcgtctgc
     9601 gctgaactgt tggctggtga agcggaacgg gttttactcg tggcccggga tgagaaaaaa
     9661 ctcgaagttt tgcgtgacag gctcaaggtc caggcaagca gcgagttatg tatcagcaca
     9721 aaaatggatg ttttgaagga cgcagacttg atcttaacgg ttaccagttc cattcatgac
     9781 gtcatccgcc cggagcattt gaagccggga agtgtggttt gtgatgtggc acgcccccgt
     9841 gatgtttctg caatggtggc agcggtgaga gatgatatat tagtgattga cggtggaatg
     9901 gttgatgtgc caggagccgt ggatttccat ttcaactttg gtttcccaga tggcaaggca
     9961 tatgcctgca tggctgagac cattgctttg gctttggaag gccgttttga agactataca
    10021 gttggcaagg atattacgtt ggaacgagtt caagatatca ctgcgattgc tgaaaagcat
    10081 ggttttcgta tgagtggatt cagatcgttc gagcgcgaag taaccataga acagattgaa
    10141 gccgttcgac aaaatgcccg caaagggtta cgacgtgctt aaaggaggct caacatggga
    10201 aacgcccgac ttttggtagt ggaagatgat gttgatatcg cgaatatgct caagatctac
    10261 ttcacaagtc tgcaatatga cgtggatgta gcgaatcgtg gccgcgatgc actggataag
    10321 acaaaacagg ttttgccgca cttgatcgtg ctggatatca tgttgccaga tattgatggg
    10381 tatgaagtct gtcgtaatct gcgcaccagt acacggacca gccacattcc ggtaatcttc
    10441 ctgacacaaa aagatgagcg cagtgataag ttacaaggcc ttgaattggg cgcagatgat
    10501 tacattacaa agccctttga cattgaagaa ttaaaactgc gtgtgcaggg tgccattcga
    10561 cgctctgaac gcgaaagcct caccgacccg cgctccggcc tgcctgccgg acggttgatc
    10621 gaagaacaat tacgccgcat tattcgcgaa aaagactggg cattgcttga tgtacgtgtt
    10681 aataactttg aagcatttaa agatgtatat ggttttgtag caggcgatga tgttgtgcgc
    10741 tttgctgcca tgatgattgg tgaggtcgtg gatgaactgg ggacgacaag cgattttatc
    10801 ggtcatgcag gtggtgataa tttcatcatt atcacaacca atgatgccgc ccccggcatt
    10861 cgccaaaaat taaaagaccg ctttgccaat gaggtgcaaa gccattacaa ctttatcgac
    10921 cgccagcagg gtttcattca ggctccgaaa gctgaagggg gcgtggaaaa ggttggcttc
    10981 atgtatcttt ctgcagggtt ggtctcgccg agccttcagt ccttcgcgga tatccgcgag
    11041 atcaccgaac ttgcggctga agcacggcga caggatacag ctgccacttc tgctgcctga
    11101 taaaagcgag cgttttcatg tcttccgggt tgggccttaa cctgttaatt gccgggctga
    11161 tttttggagc actggtcctg gtgattttgc gggttttggc tcgtacgccc tctgcggttg
    11221 ccccaaacat cgctgcagcc aattcttata ccatccccga attttcaaga tcaaacgata
    11281 acgacgccat tatcatcctc gcgcccggtg ggcgggtgga atatatcagt gcgcttgcgc
    11341 gtacattttt cgatctgcgc gagaacgagc cgtatgacct tgagcgcctt tctcggcgcg
    11401 cccgtccttc ggatgatttt cttgacttgt gtgtctcttc aggatacaag cgcctttcca
    11461 ttaatggcaa acttgtagaa gtggcttctt atgaggttcc ggggacattc ccggttatgt
    11521 tgatctcact gcgcagcaag gaatttgcac ccgcccttga gcaaagtgac ggcgcttcag
    11581 aagagatttt gcgggtggtg actgaattta gccaaagcat tgccaccagc cttgatcttg
    11641 aaaccacggc tcaatccatt ctggataatg ttgggcgtct tgtgccgtcg gatgtgcttg
    11701 aattgaaatt atggaattct gaagatcaag atttggatct ttaccgttcc caatatgcca
    11761 atctggggtc caatggctcg ggacggatga tgacttctta ttttggaaga ctgactcgcc
    11821 agttggttga aagccgtaca ccgattctgt ttggagatgt tcgttcccgc ccggatatga
    11881 atgcaaatga ggaagtgctt ccgattcaat catatttggg tattccgctt attgcaggcg
    11941 gggaactggt tggctcactt gaagctggtc aaacgggaag cggggcgttt ggccagtatg
    12001 atcttaattt gctgttactg gtttcaggtc aggcggcggt tgcgattcgt aatgcaaggc
    12061 tgtacgtcaa tgagcagaaa cgtgcttctg aattggcagg ccttgcaaat ttgaatcaaa
    12121 ctcttggggc tgtccgcgat atgcaggagc tctttacaag gctcgtggat agtgtggctc
    12181 ccttgtttca ggctgagatt atcggctttc tcctctacga tgaagaaaaa cataccatgg
    12241 aaggtaaatc tcccttccgc ggtttgcctg ctcatctggt tgatatttat cgtgtcacag
    12301 tccctgcaga aagcctcgct gaaaaggtga ttaccggcca gcagcccata ctcaccctga
    12361 atgcagctgg agatgaaaac tggcgcgcgc ttggtctttc tgatattgcg atggccgcca
    12421 gtctgcgtga tattgccctg atgcctttgt tgtcttcaaa ccgtatgctt ggttatttgc
    12481 agatcgggca tcatacccgt ggggcatcca attttggtgt ggaagaagtc cgtttgatga
    12541 gtattgtggc caatcaggct gcagcgatca tcgaaaatgt actgcttgtt cagcaagcgc
    12601 gtggacgtgc ccaacgttct gatgcactgc gccgaattgc cagtctttca ggttcttcag
    12661 ccacattgga agagatcctc aagttttcaa ttcgggaatt atcgaatctt ttccaggcgg
    12721 atgcaggtgc gatctttctg atggatgagg cacgtggaga gttgcgcctg aggcgcgaag
    12781 ctacgttcgg gcttccaaag gatatcagca gttctttcat ccagatattc atggatgatc
    12841 ccagctatcg ttcaacagtt tccggtagtc agaagccttt catttcgggt catctcagta
    12901 cagatcgtcg agtgttgacg gtgtatcgtc cattgaccac tacactttcg cttgagtcga
    12961 ccattgttgt gccattgatc gtgcgcgatc gcagcatcgg tgagttgatg ctgggaagtt
    13021 tcaagcccga ttatttcaat gcctatgatc tgcaagtgat cgccacggca gcaggacaat
    13081 tagccaccgc tgtggaatct tccagcctgc tgaatcaaac ggatgattcg cttcgtcggc
    13141 gggtggacca gctttcggcg gtgacaagaa tcggccgtga attgggcgcg tcgcttgata
    13201 tttcgcatct gattaagacc attcatgagg aaagtctgca catcgtccag gcgacttgtt
    13261 cttctgtttt cctgttccgc caacaaggtg aatccgctgg tgcagaaata gaattctctg
    13321 ccgggtgcgg aagccgtgaa ttatcgccag ccgaaacaga tgtgctgatg actcgggagc
    13381 cccgaatcat tgctgagttt tcgcagaatg accaacccct tccgcacgat ggcgtgcgtt
    13441 ctgctttgat cgttccaatc acgtatcagg cacgcttaat tggtttgatc agcctgcact
    13501 cgaacaagat caacgcatat tcagccagtg atattgaatt ggttcagatt ttggcggtgc
    13561 aggcagggat ttcattaagc aatgcgcaac gttatcaatc tgagaagaga agcgcagagt
    13621 taatgcgccg tcgctcagat acgttggtgc gcctgaccga cgtaagttac aacctcggcc
    13681 acgaccaacc cctggatcag gctttgcaga tcattgcacg cggcatccgt gagtcgacac
    13741 ctttccgagt ggtgttgatg agcatggtgg aaaccgaaac cggtatgctg cgccgtttga
    13801 ccgccgttgg cattccgcgc gaaaccatga atgagttgat ggcacgcaaa caaccgttgg
    13861 ccagccttca acagttgatg cggcctgaat tcaagatcag ccgctcctac ttcattccga
    13921 tcgatctgtc tcctgtgatg ccttccgatg tgcatatggt tacattggaa gtttcgtcgt
    13981 ctgcagaaaa gacacccaat tcatgggatc cggacgacgc gcttcttatt ccgctcgaaa
    14041 atgcggaagg gcagatcgtt ggtttgatca gcctcgatga cccatccaat ggactgcgcc
    14101 cagaccaggc aaccattgag tctgtggaag tttttgccgc ccaagctgca cttttgatca
    14161 gcaataccct acggcagggt gagttgcgca atcgcatcga ttcgctgtcg gcgggtttgc
    14221 agcgccagca gaagttgatc gatatcaccc aaaacgatct tccgattttg cttcataaag
    14281 accttgagca gaccatttct ctttacaacc tcgaccagcg tgctcaaaga gtgcgtgctg
    14341 gccttgcgat caccaaatcc gtcagccgcc agttggatgc ggtgtctgcg ctcaatgcgc
    14401 tgggccgcga aacattgacg cagttgggca tggcgatcgc cctggtagcc gaaaacaccc
    14461 cggacggtcc gcgtttgatg catgtacttg gcagcctgcc gcgctccacc aatgtggagt
    14521 cgttgttcgg tcaacgcaac ccgctgcgtg catgcttgca gacaggaaat ccaattctca
    14581 tttcaagcct tgatgaaaat gatgaatggc gcgatgccac cttgctcacc tccctgcggg
    14641 caaaaagcgt catctgttta cccgtccttg tagaaaacaa aactgtggct gccatgctgg
    14701 ctattagtca tgaggcattg cctgatttca ccgatgaaga cagcaaggtc tatttgcaga
    14761 tctcgcagca ggcctctctc attttgcaga acatttcatt gctcagccag acgcgccgcc
    14821 gccttgatga ggtgaatctg ctgctcgatt tcagccgcca gctttcgggt atggatccag
    14881 atgcgatcgt cagatccctg ctcgatagtg ccatccgcgt tctccaacat gcacatgcgg
    14941 gtgtggcgct ggtttggaat caacagactg aaaccttggt tccgcgcgcg gctgctggtt
    15001 acgctgataa caccaccatg ttggccatca actatcggca gggtgaagcc ctgccgggca
    15061 ccgtgtttat gaatcgcttt gcccgccgcg tggacgaagt gaattttgtt cgcgactaca
    15121 acctgagcac ggaaaatctg gctttgtatc gtcaggccac aggtggacgt ttgcccgtat
    15181 ccagtctgct tgtcccgatc ggtactgctg accagaatct gggcttgctg gtgttggata
    15241 atttcaacgc aacctctgct ttctcacatg aagatgaagc cctgctgctc tcgcttaccc
    15301 agcagatcgc gctctccctt gataatgttc gtctggtgca agccacacag gaacgcgccg
    15361 ggcagttgca tgccttgaac aatgctgcag cttcactcac ctccagcttg cgcagcgacc
    15421 aattgatcaa tagcctgctc gatcaactgg tccccatcat tcaatacgat acggccacat
    15481 tgtggctgcg tgataaggac cgcctcgcag ttgtttccgc gcgcggtttc cccgatacag
    15541 aagaacgcct tggcttatca gtggccgttc aagacagtgc ccttttcaag gaaatggcgc
    15601 agacaggcca acccattctt gtaaatgatg tgcgtgaaga ttcgcgcttt atgccggtgg
    15661 atgctccccg gctttcatgg cttggcattc ccatgatctc caaaggtgaa atggtgggag
    15721 tgctcgccgt tgaaaaatgg caggcacact attacacacg tgagcaaatt caagtgagcc
    15781 tgacctttgc aagtcaatcg gcggtttcgc tcgataacgc ccgcatgtat gaagacagcg
    15841 ttagccgtgc cacagaactc gaccagcgtt cacaacgcct tgccacgctt aatcgttttg
    15901 cctctgccct gaccggcctg ctcgatactg accagattct gaatgtgact gccaacgaat
    15961 tgcttaaagg gttgggcgca aaacgcgtct ctgttgtgac ctttgaacgg ggtcgggcgt
    16021 actggaagat ttcctccccg cgaacaagga tcaaactgcc gcgcctgctg cccgattcac
    16081 ccatcttcag ccgcctgcgc gaatcgctgg gtattttcaa caccgatgat gcccgcaatg
    16141 aacctgatct ggttccgctt gaagaaatgc ttggcgacaa cccaactgca ttattggtcc
    16201 tccccctcac cagcggacaa aacctctcag ccctcatctt tgcccaaaca tcaggcgatg
    16261 cgcgctttgg gttcaacgag cttgaaattg caaagaccat taccaatcaa gccactgtcg
    16321 cgcttgaaaa tgcccgtttg ttccaattat ctgtccgtac ggcagaacgg ttcgccatcc
    16381 tcaatgaaac cagttccctg gtaaattcaa gcctgaatca agaagatgta tatctggctg
    16441 cccgtaaagc ggcagaacgt ttggtgccgt tggattcctt cgtcatcacc ctgctggata
    16501 ttgagaagaa cgaaatcgac cccgtttaca tgatcgaccg tggaaaacgc atttctgccg
    16561 accgtgttcc ttttggtcag gggatgagca gtcaggtcat ttcaaccggc aaacctgttc
    16621 tgatctcaaa tagcgaacag gcgaatgata tcaacaccgt actggctggc agcagcgatg
    16681 acgaagaaga gacccgctcc attgtggctg tgccaatgac cttgggcgga aaaacacttg
    16741 gcatgctctc cgctcaaagt tatcaggaag gcatgtacac cgaggaagac gtccagatcc
    16801 tcggaacact tgcaaatcag gttgtggtcg ccattcaaaa cggacggctc tttgcagaaa
    16861 cacagagcct ggcttcccag ttcgagaaac gcgttattga gcgtaccgcc caattgcagc
    16921 gtgaacagca gaatacagaa acccttttgc gcatcctcac agaggtttcc tccagtctcg
    16981 atctggaccg tgcccttaac cgtacattgt cattgctaaa cgaagccatc ggcgcagaac
    17041 agggtaccat tatgttattg catgccgagg acaacctttt gcattaccgc gcaggctatg
    17101 gctacctttc agatcgcaac gataccagtg gacgtggctt caccttgaga gtcggtgagg
    17161 gtctggctgg ctgggtcgtg caaaaccgtg aagccgtttt gatcgacgat cttcacgagg
    17221 atccgcgctg ggtgagaagc tcagcctccg gtatggatca tcgcagcgcc attgtggccc
    17281 ccatgcaagt gggtgaggat gtgatcggtg tgctcatggt tttccagcgg gccaccaatt
    17341 tcttcagttc agaaatgctc aatctggtcc gtgccattgg cgggcaggtg gcggttgcga
    17401 tcaacaatgc tcatctctat gagttgatcc gtgaccaggc agaaagactc ggtgtcatgc
    17461 tccgcaagga acaggaagat gccagccgct cacaagccat cctcgaagct gtggcagatg
    17521 gtgtgctcgt taccggtgcc gataaccgca tcacctttgt caacacttcc actgagcgca
    17581 tccttgcatt ggatgaagca cgcctgcttg gcaatccgct tgatgtgttt ggtggtctgt
    17641 tcggaaaagc cgccagcacg tggatggaaa ccatccgccg ctggtcggag gatccgtctt
    17701 cctatcaatc tggtgactcg tatgccgagc aattggaatt ggaaaatgaa cgcatcgcgc
    17761 tcgtccacct tgcgcctgtg attttgcaaa atgattttct cggtaccgta tccatcttcc
    17821 gcgatatcac gcatgaagtg gaagtggacc gcctgaaatc ggaatttgta gccacggtca
    17881 gccacgaact ccgcaccccc atgacagcca tcaaaggcta tgtggatatt ctgaccatgg
    17941 gtgcggcagg cgctctcacc gaaaaccaat tgcacttcct cgatattgtc cgtaacaata
    18001 tcgaacgtct caatatcctc gtcaacgacc tgttggatat ttcccgcatt gaagctggac
    18061 gagtgacctt gaacccgcaa tccgtcaacc tgcgcgagat cgccgaagat gtcatcgcag
    18121 agacgttgcg ccgttcacag aacgaaaata aacccatggc cctctcactg gatgccccgg
    18181 cgaagatgcc ccaggtcatg ggtgatagcg accgtgtccg tcagattatc ggtaacctgg
    18241 tggataacgc gttcaattac acgcccagca atggcaccat ccgcgtgaac attcaccagg
    18301 aaaaagatga agttcaggtg gatgtgcagg acaacggtgt gggcattgca ctgcaagatc
    18361 aaggccgtat ctttgaccgc ttcttccgcg gtgaacaccc ccttgttctt gccacacccg
    18421 gaacagggtt gggattaccc atcgttcgcc agctggtcga aatgcatagc ggacgcatct
    18481 ggatgacaag cacaggtgtg ccaggcgaag gcagcacatt ttccttcacc ctgcccaccg
    18541 tacataatgg agtttgaatg gcaaggatct taatagcaga agacgaacca gatattcgtg
    18601 aacttgtcgc gttcaccctg cgctttgcgg ggcatgaagt gacggcaacc gccaatggag
    18661 aagaagccct gcagcaagcc gggcagatca ttcccgatat catcatcatg gatgtgcgca
    18721 tgccgcgcat gaccggctac gacgcctgcc gtgccatgaa agctgacccc attttgaagg
    18781 atatccctgt ggtctttctt tcggccaaag gacaggattc agagatccaa actggcctgg
    18841 aagcaggcgc tgaggaatac ctgctcaaac ccttcgcccc cgaccagctt tcagaacgtg
    18901 taaaggcgat tctttcgaaa ttcggaaaat aacgattcaa tgagcgcaat cattctcgac
    18961 ggttcgatgg tgcattatga ggcattggga cgcggacgcc ccattgtctt tctgcacgat
    19021 tgggtcggct catggcgata ctgggtcaat gccatgcaag tcgcatccac atcgttccgc
    19081 gcctatgcgc tcgacctctt tggttttggc gatacttcac gcgacccatt gcgctattcc
    19141 ctcgataaac aatccgaact cgtcaatcgc tttttgcagg aaatgggtat cggcaaagtg
    19201 gcattggtgg ggcatggcct cggcgccctc gttggtttta cctacctcaa acaattccca
    19261 tcgaccgttg accgtatgat ggcagtcaac tgtccgtttg aatatgattc ggtcaatacc
    19321 cgcctgcgga ccgctgccat gcctgaactg gtcgattggc tggcaggccg cacccccgaa
    19381 gctgtatccg cactttccga tgcggccaaa gccgacccgc aagctgtttc tgcttcgatg
    19441 gccagtttgc agggaaataa ctacttcgcg gccattcgtg atcttggtgt tccctgcctg
    19501 ttcgcctatg gctcggaaga ccccgccatc agcatgccca ccccagaccg ggtcgattcg
    19561 cttccaatgc acatgcatct ggtcacgctc gaagaatcaa gtcacttccc gatggtggat
    19621 aaccccaccc gcttcaaccg cctcatgacc gatttcctcg ccctagattc aggggccagt
    19681 ccgcgcgaac tgcaaatgaa agaagaatgg aaacgcaggg tgcgttagga agaaaagaaa
    19741 cttgaattga catcccgccc tgagtataaa aaaacagcct cccgaaaata gggaggctgt
    19801 ttttgaacca gacaaagccg actagccgcc gcttcccacg gcattgatga tgatgcccaa
    19861 aacgatacca gcgatccaga cgtacattgg ggtcttgagt ttgagttctt cattcttcat
    19921 attttgaaga ccccagataa aggtgtagag catacaaagg aagcccaaaa tgcccttcat
    19981 cacaccttct ttctggaaca acttgatcca aacccaaatg cctgcaataa acgcaaggat
    20041 tccaccaaga ccagtattca ttgaaaattc catttcacac tctcctctta aagttgatgg
    20101 gatttatgca atcaggcgaa acgcccaata aagcactaat gagaaaaaca cccatgcagt
    20161 aaccccgtta cgtgtttcaa ggcggtttac gaaactgata attttttttc ttgctggttc
    20221 gggcaatatc aaaactgaaa tcatcaataa cagagcagca agaaagagcg gcgcaaaagc
    20281 gtgggtttga agtgatgcag cgacctcacc gtgaaatagc tgcatggtgg cacgggttaa
    20341 cccgcagccc gggcagggaa cccccgtagc ggcaaggatg gggcaggtcc aaaacgagat
    20401 tcccgccagg cttaacccca aatgcacaac cccaaacccg attatggctg tggtttcagt
    20461 gcgattggtt aatagagttg aaaaaacagg ttgatgccag agtgttttga ttgaatgttc
    20521 catgagagtg gtaacacatg gacacccaaa aggatgtcat gaccaaaaaa acacaaatca
    20581 agcagatgaa cccatcacag cgaaaacatt gatggcctga ttcttgccct tgaaaacgat
    20641 cttccccaaa tcctccacca gaatatcggc aggcaggttt tggcgggtgt agttatcgat
    20701 caagatcggg tgcagggctt cctttgtgta ttcttcaatt cttgaggcca catttaccgt
    20761 gtcaccgatg caggtgtaaa tggcacgcgt ttgagtcccc gtgtagcctg ccaccatctg
    20821 acctgtggcg atcccaatgc cgatgcgaat ttgggtcttg ccgtgagccg cctgattgtg
    20881 attgaaaacc ttcaactgtt ccagcatttg cagggcggcg cgcacagccc tttcagaatg
    20941 gtcttcaaaa aagatcggtg caccaaaaac agccatcaat ccatcgccca ccacctgatt
    21001 gaccgtgccg ccgttgttcg tgatcgcatc gaacatcagg ctgaaataat gattaagcag
    21061 gtcaatggat tcagatggct cctgattttc agcaatgctg gtaaacgagc ggatatcggc
    21121 aaacatgatg ctggctttta cctgcttgcc tccaagtgaa aaacctgaac gcagcaattg
    21181 ttcggccact tccttggtgg caaaagttct aaacaggcgt ctttgttcat cacgcaggcg
    21241 ttttttttcg aggctggcat tcacccgcgc acgcagcaag atcgggttga cgggtttcgt
    21301 aaggtaatcc tctgcaccga gttcaacgca tttaaccacg gcatccagct cttccatcgc
    21361 tgaagtcatg ataatgggga tctgccgaag gtccacatcg ttcaaacagg cctcaagcac
    21421 ctcaaacccg ttcatctcgg gcatctcaat atccaacagc atcaaatcga atgtgcccgt
    21481 gcggaattta tccaacccct ccctgccgtt ctcagcggtt tccacaatat gcccctgctg
    21541 ctcaaggctg cgcgaaagca ccatgcggtt gaccttgttg tcatccacga tcagcaaatg
    21601 ggcgggtttt ggagattcag ccattggatt tacctcattc ccagcagttg acccctgacc
    21661 tgttcaaacg catcctgcag gacctcgagc ctgttcccga tttcgaggtt tttttcacgc
    21721 ccgagcattt ccaactcgcg tgcgagtccc gcgagctcgg tggcaccaaa tgtcgccgca
    21781 ttcgatttaa gcgaatgtgc cgcacgccga aatagatcca catcctgtgc agccaggccg
    21841 ctcttcattt gcccgatctg tgtgagggca tcttccaaaa atgtatcgat catttcaccg
    21901 atgaaatcgg cgcccaccgt ctctttcaaa ttagtaaatg tatttaaatc aatgactgac
    21961 atttttctcc atttgtttga ttatcacatc agaaagcctt tacggcctga agttagctga
    22021 ctcttcttgc tttcagtaac gcatccacca attccaccac gcggatgggt tttggaatgt
    22081 agtggttcat gcccgcacca aggcacattt cacggtcgcc ctgcatggca ttggccgtca
    22141 acccgatgat gaagggacgt ttatttaccc agcgtgccac gatctgacgg gtggcctcaa
    22201 gaccgtccat ttcaggcatt tgcacatcca tcaagaccac gtcatattgc tggcgttcga
    22261 tggattgaat ggcttcaatg ccattcgagg ccacatcggc acgatagccc atttgctcaa
    22321 gcaggcgcag cgccagcttt tggttgacag cattatcttc agcgaggaga attctaagcg
    22381 gatgccgcac agccatttgc gggtcgggtt tgaagcggcc tgtcgtcggc ggctcctcgt
    22441 aggctgaggg tctgaaaaag agtccaacca atgtatcaaa aagctgtgat tgcttgatcg
    22501 gcttggtaag gtaggcggca aagacgttgg tttcatcgcc cgcttcacgc cgacccagtg
    22561 aactgaaaag caccagcggg aattttgcct ttgcacgaat ctttgttgcc agttcgaggc
    22621 cgtccatttc gggcatgtgc atatcgagga tggcaacatc aaaggcttca ccctgttcga
    22681 tccatttcag cgcggtgagg ggcgaggcgg tttcgtgcgg gatcatgccc catttggaag
    22741 tttgtgcgtt gaggatgtag cgattggtgg aattgtcatc cacaatgagt acgtgcttgt
    22801 tttgcagttc gggttgaatg cccgaatgtc cgcgcttttg ggcggggaga atttctgcga
    22861 gttcagcttt gacggtaaag atgaacgtgg agcctctgcc caatccgtca ctggtggccc
    22921 acatggtgcc gcccatcatt tcagcgagat gtttgctgat ggccaatccc aaccctgtgc
    22981 cgccatactt gcgtgtggtg gatgaatcgg cctgtgaaaa tgattgaaag agtcggctca
    23041 tgccgtcttt tgaaaggccg atgcctgtat cgcggacgga gaatgtgagc agggcttttt
    23101 cttttgcgcc gccgttcctg tagcgctgac tggaaaccgt gagcaccact tcaccttttt
    23161 ctgtgaattt gaccgcattg ctgagcagat tggtgatgat ctggcgcagg cgggttacat
    23221 cgcccacaat ggcgggtgga atgtcgcctt cgaaaatgta ggcgaggtcg atcccttttt
    23281 cgacggcccg agcagaaacc aggtccagtg atgattcgac acaatcgcgc aggtcgaagg
    23341 ggtgatgttc gatatccatt ctccctgctt cgatctttga gaaatccaaa atgtcattga
    23401 tgatggccag cagggcatcg cctgaattgc ggatggtttc ggcataatca cgttgttcat
    23461 cgtcgagctt ggtgtcaagc aggaggccgc tcatgccgat gacggcattc attggggtgc
    23521 ggatctcatg actcatggtg gcgaggaagg aactcttggc ctcgttggcc tgttcggcgg
    23581 cggcgcgagc taggcgggca tcctcaaaga ggcgtgcatt ctgaatggct gtcgccatgc
    23641 tggaggcgat ggattccatc agggtgatga tggattcatc gtaggcgttt tcgcgctcaa
    23701 agttttgaat ggtgattcca ccgatgaggt ctttgccgac caccatcggc accatgatca
    23761 cggatttggg gacctcattg agcgccgtca tgttggagcc gaggcgcttc cacaatgaag
    23821 gggattggcg gttgataacc agcgatttac ccagcatgga tgcgctgatc gtggaatcgt
    23881 tgatggggaa tgagggcgtt tgaatgcgtt cgttcttgcg ggtgacataa tgtgctgtga
    23941 tggagcgcgc ctcggggtca tataacccga tgcctacatt gtcttccatc accagttcgc
    24001 ggatcttatc gccgacacgt tcgacaatgg tttgcacatc caattgctgg gtgagcacct
    24061 gtgcaatgtt attgaggatc tccaattggg aggcgcggcg tttggtttcc ttgagcaggc
    24121 gctgggtttc atcgaagagg cgcgcatttt caatggtcac gctcaatgag ttggcaatgg
    24181 tggtgatcag gcgtacatcg gcttcgctga aggcttcttc atgctcaagg ttctgtagag
    24241 aaatgacccc gcgtacctca cccgccaccg acattggcac ccacacaccc gaacgcacaa
    24301 ccacatcccc ggggttggct cccagcaaaa ccgagttgta cttttgcgaa agttcgtcga
    24361 agttgcggtt ggctacgatg ggctcacctg tgcggatcac atgtccgctg aacccgccca
    24421 tgccatcttt caataccagt ggctcctgat aaaaacgctc gccgttctcg aaaatgtacg
    24481 ggaattgcgt gatgttggtt tccttgtcgt acatggttag cacaacggtt tgggcatcga
    24541 atattttttg gatctggtcg ccgacatcgt cgtagatggt ttggatatcg agtttggggt
    24601 caagacctga gatcagcgcg ttgatgatgg aaagctcatt ggcacgcttg ttgatgaaat
    24661 cgaacatgct ggcgttttga atggcaatgg ccgcctgcag ggaaagccgt tttagaaaat
    24721 caaggtccgc ttctatgaag gggtgaccgc cattgcgcca tacggccatc atgccgccga
    24781 ccttgccgcc ggtcagcagg ggggctgcca tcaaacgttc ctcagtctca accggggtgc
    24841 ctggaatctg tatggtgcgg gggtcatggg cggtatcgtt gatgaattca ccctgccccg
    24901 ttttggcgag tccgccgatg atgccctggc cgaagtaaat ggtatccacc ttgatctctt
    24961 cggcgatctc tccttcggcg gcaatggctt tgaagtatga gccgtcacct tgcggtagat
    25021 aaatggcgct ggtggatgcg gagagcaggt cacgcgcatg gtgggcgatc ttgtccatca
    25081 ccgccttcag atcaagcgtg gatgaaatat cgcgccctac ttcattcagc accgaacttt
    25141 cacgtgcgcg gcgctgggtt tcatcgaaaa gacgggcatt ctcaagtgca atggccattg
    25201 agttggccag ggtttgtagc agacgcaggt ctgattcttt gaaaacgttt tccctatcta
    25261 cattttgcag tgaaatgaca cccttcacct gacttcccac cagcatgggc acatccatgc
    25321 gcgatttgat atcctctccg ccgccaagga tgctggaacg cgtgccaagc aggcgttcct
    25381 cttcattgga catatttgcg ttgatgataa cgggttggtg ggtacgaagc acataccccg
    25441 aaataccgct ggggggtaaa ggttcctggt tgaggcgttt gcccatttcc attaaatagg
    25501 gaaaggagca gatgttcttc tcagcatcgt aggaaacgat catcacgacc tgtacatcga
    25561 agatccctgc gatcttttca ccaaccaggc tgtagagcgt atctccatcc agttgtgaag
    25621 ccagtttggc ctgcacactg ttgacgatct ccaactcagc cccacgctgg gtgctttcct
    25681 ccagcagccg ctgcgactcg ttgaaaagac gcgagttttc aatggcgatc gaagcctgat
    25741 ctgcgaataa ctgcaaaagg cgtagctcgg tattggcaaa ggcacgcgct tcagtgaagt
    25801 ggatgttcat cacacccact acacggtggc ggaatttgag cggcaggccg atcaacgcac
    25861 ccgtccaacg cgctggtgaa tccttgtata gcggatgctt acgcatgtct tccaccaaca
    25921 tgatctcgcc tgtctttgcc acggtgtcgg tcattccacc gggacggggt gctgaaacag
    25981 gagtgctttt gatctcaccg ttggcgatgg ccgtgccaaa taccagttca tcgttttcgt
    26041 acaggaacag gttgatgttt tcaatgctcg gcattaattc agatgtgctt ttcaggatcg
    26101 aatccaacac cgtggaatat tcaaggttcg acgaaaggtc aatgctggcc tttcgaattg
    26161 cttcaagctc ggcattgtgc tgctcggttt ccttgagcag gcgctgggtc tcgctcaaca
    26221 ggcgcgcatt ttccattgcg gcactcatcg aacttgccag agtctccaac aggcttacat
    26281 ctgatggact gaaggaattc tcccggtcca tgttttgcag tgaaatgacc ccgcatactt
    26341 caccgccgct gatcagcgga acgtacaggg cagattttgg aatcaatggc cccagcacga
    26401 gcttcgcccc atacgcctcc atctgttctt tgacatggtc gttgaacagg aaggtttttt
    26461 tcaaccgtgc gatcgttttt gtaaaaccgc tcatggcagt gggttccagg ttttccctgc
    26521 caaagcggtc aatgtaataa ttgaaatact gggtctcggt tttcaggtca aatgatgcaa
    26581 taagggtggt ttgtgcgcca aagatattat gaatcttatc ccccacaagt tcatacacag
    26641 cctgcaattc cagctttgaa gccaggccat cctgcacact cttaatgatg gccagctcag
    26701 cttcacgctg ctttaactcc ttgcgaatgc gctcacttga acctgatctt gaagccgcag
    26761 aagttggttt ctttgccgtt ttgggcgttg ttttttttgc tttttcggtt ttaggcatgc
    26821 gcacctctaa gacagaattc agtttacata cgggcgggaa aatcgtcaat agcggaaata
    26881 aaacaaacag gcgaatcggc ctgtttgtca ttgttttgaa acggattttg cgcttcagac
    26941 tcagggttgg cctgcctctg tcacccaggc aggaatcggc cctgacggat caaaaatatt
    27001 gggcgtaagc ctcacccagc agcttttcca gatacggcct gacctgccag aacgatttca
    27061 tgttgcggta gtcttccacc cagcgcggat cgccaggttg ggtggtcttc aaagagcgga
    27121 tcatcagatt cttgcccgtg tgttcaatgg ggacgaattg agttacatcg gtgcgatagc
    27181 ccagcacacg cagcatggtg gcacggaagg tgtctgtcag gatatctccc atgcgttcga
    27241 acaaaatgcc gtgatgaaaa acaggctgca tctggctggg cgttggcgcg tgggataact
    27301 gctcctgtaa ttcatgctgg cagcatggcg cacagacgat gaccttgctt tcccattgga
    27361 ttcccttggc gagcgcgtcg tcggtggcgg tgtcgcaggc gtgcagtgca aggatgacat
    27421 tcggcttcac ttcaggttca tactccgcga tataaccgac ctgaaaagat aaaccgtccc
    27481 accccatggc ggcggcggtt tggttgtgac gctcgatcaa ctcggctttt acatccacgc
    27541 ctgtaacgcg agcgttgaga tttaaaatat ttcgcaggta ataatagacg gcaaaggtga
    27601 ggtgggcact gccgcagcca aagtccacta cgttgatggg tgccccgctc caggcggaga
    27661 aagaagcggt ctcttccagc aatcgcaaaa attcattgat ctgtttatgc ttgcgctgca
    27721 tatccgcttt gatgcgccca tccctggtca tgatgcccac tgcctccaaa aatggacgcg
    27781 cgttttcatc ggagaggatg cgatgctttt cacggttgtg tgcaaggttg accgtgagcg
    27841 gcactgcggc aggtttgaac tcgttgacaa tgggtttgcc ttttttcgaa acattgacct
    27901 gcacattgcc tgcattgctt tccacaaaca tgttgcggaa gggcagcgcc agcaagtctt
    27961 ccactttggc tttggcttgt ttgaaagtgt agttttgggt cgtgtctttt ttctcgtcga
    28021 aatacgagaa ctgcagttga acttcacctt gaatttcaac agggcgtacg atgacctttt
    28081 cccactggat ggaaaacccc ttttgcccgc cgctaaatgt ggcgcggacg aaggtttcgc
    28141 gggcaaggat gcgttctcga atcagttgct tgtaatcttc catgccgcga agtatatcat
    28201 ttgcgcgtta aacagaatgg gggtggctaa aagagtggga tttgattgcc acgaagccac
    28261 aaagactcga agccacaaag aattcttggc gtctttgtgt ttagcccgca gatttagcca
    28321 ctctcaacag catcagtgtt cgattcgccg cttaaggcat gggcatgcag ggtggcacgt
    28381 acccgctcca ttaattcggc gttggtttgt ttgcggttcg ggtcgccgta gccgtaatca
    28441 tcggtataaa ttttgagctg ctttattgct gtgccgcctt gatacaggtc aactaaaaaa
    28501 taaaaacggc cataacgcgc ccattcccaa tctatgaatt ttgctgtcca cgaaagtccg
    28561 tttccaagat cggtcggttg gggaaaatcc tgcatgtctc ctacacttcc ctttcctctt
    28621 cctcttttgc agcctcgccc cgttccattt ttttcttcaa atgctcgggc cacgcgctga
    28681 cattttctga ctgcaagaac acagggctgg ttcctgtgtg ggtcaaaata tcccgtgcgc
    28741 ggcaattgag cacggctttt gccgcatcaa tgcctgattg cgtcacccct gccaccccgt
    28801 gactgatggt gctttgcccg cacagataca agccttcgaa ttcggtgcgc ggtgtaaacc
    28861 caagcggacc cacttgcgat ggacgtttat caatgccgta taaatttccg cgggtggcgt
    28921 tgaggtagtg ttcattcgtc aatggcgtgc cgaggctgta gaaggtgatg tgcttgctca
    28981 ggccagggat tcgtttctca agtccgcgca ccatgcgcca ggcgaggtct tctttcatgg
    29041 cttcgtaatc ggcagggcgt gcgccgtatt ttgaattcgc ccatttctca aaggcatcgt
    29101 atcccacaaa cgcaaacgac tcgcaagtgt gttgtccgtg atgcatcttc gaggggtctt
    29161 tcagcgtcgt tgtggtcagg aacatgccgg gcggagtctc atcgttcaga acggcatcgg
    29221 tgaggccctg ggtgtagatc ttgtccacgt cggcatggtc gtaataccac atgttgcccg
    29281 agtcgaggcc cgcatcgcgc cgaaggtcca tgtcggtggc gaagaaaagg ctgaggcagg
    29341 atgttgaata ttggacagaa tcaattttcc gtttgagtcg agccgagagg ttttccttgc
    29401 cgatcaattt tccaaaggtc acttccgggt cggcgttcga gatgacgaca tctgcgcgga
    29461 tctcttcacc cgaatccaat tccacgccca gaactttttt cccttcgagg agaatccgct
    29521 tcaccctgca ctgaagcctg atctcacccc cagacttttt cagcgcccga acaaaagcac
    29581 gcggaatggt gaacgcgccg ccgagcggat agaagccgcc gtcaagatag tggtgggtga
    29641 tgccagcatg gatgaacgcc gagacctgcg aaggcggcat gccatgatcg cccgattgcg
    29701 ccgcaagtac gccgcgcaac acggggtcgc tgatgtgtgc gttgatcaaa tccgcgcctg
    29761 tggaacggat ccatttcagg atgtttcccg cgctttttgc ggtttcaagc gggttgctca
    29821 aattgccgat gcggcgcagg ccttccatca tattggtgag atcggtgaaa tatgagtcga
    29881 tcccctttgc ctcatgcgga aaatggctct tgagccgctc gattaaattt tctttacctt
    29941 tggggaaatc aatgcgtttt tcgccgatga agatatggtc atatccatcg gggttgagtt
    30001 ctacgaaggt caggtcttgt gaaacgccca gtccgtcata gatgcggcgc aacccgccgc
    30061 cttcacccag tgaaccaatg taatgcacac ccggactgaa gcgatagcct tcaagcgtga
    30121 atgaatgagt ccagccgccg gggcgatcat gctgctcgag gacgatcact tttttacccg
    30181 cctgcgccaa tgcaacggcg gcggtcaacc cacctgcacc cgagccaatt acgatgacat
    30241 catacgtgga catgttgcct ccagatcggt tatctattta caggaatttc atttatcagg
    30301 ggagagattt atcttacttc actcattcgt catcaacttg acgtaaattt cctccagcgc
    30361 cgattcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt acttcactca
    30481 ttcgtcatca acttgacgta aatttcctcc agcgccgatt cgcgcatgga gaggtccatc
    30541 agggtccatt tgttggcgga gacggtttcg agcatgtggg cgattccatc cacctcggca
    30601 gtgcggaaga tgtaattgcc gttgacttct tcgtaatcca gtttttggtc ggcgtgaaag
    30661 atcacctgat attcgcgctt cccgagcctg gcacggatgg cttccattgt gtcgaagatg
    30721 agcagtttgc cgtttttgat gatgcccacc cggtcgcaga tggtttcgac gtgaaagagg
    30781 ttgtgcgcgc tgagcaaaat ggttttgcct tcgcggcgca gggttttgag gtaattgatg
    30841 atgaagaacg aggtcaatgg gtcgaggcct gaattgggct cgtccagaat cagcaggtcg
    30901 gggtcatgga gcagggcgcg ggcaatggcg gtcttgcgtt tcatgccctt ggagaattct
    30961 cccgtcagct ttttggtttc gtgcaggccc agagagttga gcaggtcgtc aatacgcttc
    31021 aatgcctttt ggcgcggcat ttgatacagt tcggagaaaa acagcaggta ttgctgggcg
    31081 gtcatggcct catacagcgg actttcctca ggcaggtagc cgatcttttg cttgatcctg
    31141 gcattgcctt tttgaatatc ttcccccatc acacgcacca cgcccgaggt cggctcgatc
    31201 agccctgcga tcattttcat ggtggtgctt ttccccgcac cgttatggcc gatgatgcca
    31261 acgatctcgc ccggatgaat ttgtaagttc aactcgtgga cgactgtagt attatcgtag
    31321 cgcttggtga cattttctat ggttatcatg ttgtaatttt atcctattat gaataatgtc
    31381 attgcgaggg cgatagcccg aagcaatctc cgacagtgaa agagattgct tcgggcaaag
    31441 aacaagagcg ccctcgcaat gacataacga gagtcttatg aatcaaattc ttgtcattgc
    31501 ctggcgtgag atcacacggt tgcgtaaacg ctttggcggc ggggcgagtc cgctggcggt
    31561 gctggctttg ctggcagtgt tgggcgggtc atcttatttc ctgcgcgatg cggtttcgct
    31621 tggcagcggc ctgtaccgtg tgggcgtgat gggcaatgta cccacgatca acgacagccg
    31681 ctttgccgtg atggaagtga atgaggagca ggggagagtc ctgctcgaac aaaaagccat
    31741 tgacctgctg atcattgatt cgcaggtgat cgcgcgtgaa gatgacaagt cacagtttgc
    31801 ggtgcgggcg ttgaaacaat acatggaggc cgaggaattg aaccgtgtgg ggaattcctt
    31861 ctctgaagct gacgcttttc cactgcgggt ggcgatcaac tacctcggag ccgagcaggc
    31921 tccggtgcag ggtgaggcgg gcggaacgct tcccacggta aagcctgaag aggtcatcat
    31981 cccttcgcta acgccgccgc ctgcgccgtt tacgcaggtg ctggttgccc tgctttatat
    32041 tttgccgatc accttcatca gcattttctt cacgagcagt tttatggatg agaaaatgaa
    32101 tcgccgcctg acgatcttgc tttcggcgcc tgtttcgccg tttcagatca tcttcggcaa
    32161 gatgctgcct tatgcgattt ttgcactcgt ttccaccgcg ctgatcgctg ctctgaccaa
    32221 aacgaacatt cccctcgccc tggccatttt cgcgccgacc acacttttta tctttgcgat
    32281 ctatttgatg gtgccgttgt tctaccgtac tttcaaagac acaaccttca ttgccatgct
    32341 ggtgacaaca ttgacgacgg cataccttgt gtttcctgcg atgttcacaa acgtgaatga
    32401 ccttgcctac atttcgcctt tgaccctggc ggtgaagatg tatcgtggcg agccgtttgg
    32461 ctggcgtgag tatctttttc catccctgcc gatggcggcg atctttggct ttgcgatgtt
    32521 cgcagggacg cgcatgttga acgaggaatt tttgatgggc tacaagcccc tcacacgcaa
    32581 gatcaaggat gcgatctatt tgatgatggc ccacgcgaaa ccgtccattg ctgtgccgtt
    32641 gtggagtttg atcgtgatcc ctgctgtgta tatgacgcag atcgtttttc tggcattcgc
    32701 caccaacctg cctgtggggc tgatcctcgg ttttacattg atcgcttcgg cgctggtgga
    32761 ggaggtggtg aagacgatcg gcattgtggt gctggcggag cggggtgtgg tgaaaacatg
    32821 gcgcgagatc attggtttgt cgtttttatc ggcgctggga tttttgatcg gtgaaaagct
    32881 tttgcttttg gtttcgatca gcatggtttc ggaggcgcag gtttcaggcg cgttgtttgg
    32941 tgcgggtttg ttgttgttcg tgcctttggc ggcacatttt gtgttcgtca ccattgttac
    33001 cgtgatggcg gcgaagtgga gggttcctta ctggctggct ctgggagtgg ggacgatcct
    33061 gcattttatt tacaatgtga atgtgatgcg aggtggaatg tgattgctcc gatgttggcg
    33121 gtggctaaaa aggaactcat gtcggtcctt cgcgaccgta cgatcgtgat ctcgatcttg
    33181 atccaattgt tcatcgcttc attttcttct gcgttgttgt tggggatgct ttccctgtat
    33241 gattccgata cgatcatgcg tttcagcgga gcagggatga ggatcggctt tgtgcgtgcg
    33301 ccagttaatg tgttggaaca gttgatgggt gaacgcggtc tgaccattgt tcaattccca
    33361 tccctggggg aggcgcaggc cgcatattac cgtggtgagg tgctcgcgat tgtggaacct
    33421 ccacaggatg cgaacggcct gaccgaaaca aggatctacc tgcctgagtc agatacggtc
    33481 aattcactga tccgcatggt gattcaggag ccgctcaaac aatatgaaaa ccacctgcgc
    33541 ggacagaagg ggatcgaggt gcgctatgcc gatttgaagg gcaaaccctc aacctcgtat
    33601 gaatttgtgt attcggtttt gattcccatg ctgatgttct tccctgcttt cgttgcaggc
    33661 agcatggccg tggattcgct cacggaagaa gtggaaaaca agaccttgca aaccctgctc
    33721 tctgcgccgt tgagtgtcaa tggcatggtt gcgggcaaaa ttctggcttc ggtgattctt
    33781 tcggtggcgc aatgtatggc gtggctgacc ctgcttcagt taaatgggat cgtgattcaa
    33841 aatgcaggct ggattttgac cctggcaacc atcgtggcgg gcatcacatc cactgttgcg
    33901 gctttgggtg cggtgatgct gcaagaccga gagcgttcac aattcattta ttcgctcatg
    33961 ctgctggcgg cggcgggtat cagcaccttg ttaaatattt cgccgatcac cacgctttcg
    34021 cggctggcaa tcggcgatgc ttacacaaat ggctggaatg tgacggtctt tgcagtgttt
    34081 ctggcggggt tgtattttct tttgatcagg atgtcgcgga ggctgctggc ttgatctctg
    34141 cggtcagcct tgcaatgctt tttcatacac catgaattcc cgcaatactt cgggtacgat
    34201 ccatgcatat tcatcgcgct tcgaaccttc cagccgtgaa atggtggtgt gccacgaaag
    34261 tgttttgaca atggatgcca ctggttttga aaggcggtag gcggtttgca ggctggctgg
    34321 cgaggcgaaa gcctgccagg gttttaggta gagttcgagc aaggcggcca ttgcgggggt
    34381 gaaggaataa tcttcgaggt cgagagcgat ctcaatgctg acgaaaaagg tgcggagcga
    34441 aacgaatgga tgggttacgc tggcatcgcc ccaatcgaaa aaggtgatgc gcccatcttt
    34501 cagcaggatg ttaccgtcgt gaaaatcacc atgattgacc gattcaggga tttgaattgc
    34561 ggcaaggtca gagcagatgt tttcaaagcg tggctgcaat tccttcagtt gttgaaactc
    34621 ggtttcggtc agtccctttt ctttgccgat catcaaactg tcttcgtctt tgagtaactg
    34681 tttatacagg gtgggcagtg tacccaggcg gtgatcgggg atcccgagcg caaggacttc
    34741 atttacatga ctggcaagcc cgatctgcag atcagcataa cgggtgatga caggttccca
    34801 gggcttgatg ttttttgtcg ggcggatgga agcgcgcagt tgttcgccgc catcacgcat
    34861 caacatccaa ccgcgtgcgg ggtctgcact tatgatgtcg ggcagggaat cggggaacca
    34921 ctccgccatt ttttttgtca atgcaatttc ataaaccgtt tcggcagcgg tggctttgaa
    34981 gaagagcatc cctgcgcttg atgaaacgcg catcacagtg gaccatgcat aggcatgggg
    35041 ctgctcgatc tcacctgtca actggatcga attttttcct gcttcagacc gtatccagtc
    35101 gtgggcttgt ttttgccatt cggggttgtg ccagagttgc aggctcattt tttacttctt
    35161 ttgcgcggct cccatggccg cgccgacaat gccagcatcg ttcaataatt gagcggggac
    35221 gacgggggtt tcaatggtga gcaaaggcag gaatttttta gagtctttgc tgatgccgcc
    35281 gccaacaatg aacagatcag gccagaaaag tttttccatt gtgctcaagt atctgttcaa
    35341 ccgctttgcg tattttttcc atgaaagttt gtcgcgttca cgggcaaatg cggaggcgcg
    35401 aaactcggca tccttgcctt tgatctcaag gtggccaaac tctgtattgg ggagcagatg
    35461 tccgcggtga aagatggcgg tgccgattcc tgttcccagt gtgatcatga taacggtccc
    35521 agattgaccg cgccccgctc cgatggtcat ctctgccagg cctgccgcat ctgcgtcatt
    35581 gatcattgtg cattcacatt ctgtcacttt ggaaaaaagt tcatctgcgt tgaggccaac
    35641 ccatttatct gatacattgg ccgccatcat cgccacaccg cttttgatcg gggcagggaa
    35701 accgatgccg atcggcccgc tccagttgaa ggattgcgcg atctgtttga ccacttctgc
    35761 aatgggttcg ggctcccctt ttttgggagt cttgatgcga agccgttccg ccgctaattg
    35821 ccccgtttca atgtccactg gtgcgccttt gatacctgag ccgcctacgt caattccgag
    35881 gatttgcatg atctgttcct gcctttaagt ttatagtggg gagatttaat cacatatgtc
    35941 tgacaaagac aatgtttaat ttaaagcttt cattttcttc ttcttacacc catagaaaag
    36001 cattgaggct ttgcccggtc acggcttgca cgatccgctc gcagattttc aggcccctgt
    36061 tgccgttggt cagaatgacg atcccatttc ctgtttcagg gtctccgaac atgaagtttt
    36121 caaaacagcc attctcgccc caatgccaaa aacagtttct atcgccgatc ttttcaagcc
    36181 cgatcccgat tccccatgag agcggactgt tcaatggcgg ttgagatttg agcatttgct
    36241 ccgcgatttc atttttgggt tcaagcaaac gcagcataaa tcgagcgtat tcgggcgcag
    36301 tggtcaacaa actgcccgcc acgttgggaa tcatgttgtt tggcaggggc gatagatttg
    36361 catgaacctg tggcagggct tcgacaaaat cctggtacaa ccagtcatcc aacgccttgt
    36421 tcttttgcgc ggcgatttcc attaatttcc tgccctgcca tgcattccac ggattgactt
    36481 tttgtccgcg gtcacgatgc cccatgctga tcatcgcctc gtgctccgct ctccagatat
    36541 aagtgctatt gctcatccca aatgggcgca aaacccgatc ctgtaaaaat gattcaatgc
    36601 tttggccagt gatctgttcg atgacacgtt gtacatagaa gaaaccttca cctgaatagg
    36661 agaagcctgt ccctggctcg aattcaaact caaatttgtc ttcgatatca aaacgccaat
    36721 tctgcaggcc tgatgtatgt gtcagcgcct gacgggctgt gatcagtttt agttggggga
    36781 tctcgttcgc ttcaggcagg ggaagataat cgtggagagg tttgtccagg tccagctttc
    36841 cctcttgctg caaggttaga actccatagg taaatactgg tttgctcaaa gatgcggcct
    36901 gaaaaatagt gtcttcgttg atcgggtcat cttttatttc tgcattcttt atgccaataa
    36961 cctgagtgga tattatctgt cggttttgaa tcacactaaa tgccaggcct ggcacaccgg
    37021 cggcttccat gaattccaag agcttttcag ttggtatggc tgatggctgc atgtttgctt
    37081 ctcctgataa agtttatcaa cacctgtcca gctaaaatac cagcttactt aagggtaatg
    37141 catgaattgt acgcccttcg tgtcctgtca tggtctctgc catgcacaat gaattataga
    37201 tggcttcttc ggtggcttca ataacggcct ggaacaatgc cgacatccct gcattgggaa
    37261 cttcgggata tgcccaaatt ttcccgcgcc gctccgcggt tctgcgtact tcctccgctg
    37321 tggaaaatgc aatgacataa tctcccgagc catcgctcat ggctgcgcct gtctttgcca
    37381 atccgcttag tccacgccgt gccagccgtg agaggtttct atctgaaagc ggcgcatcag
    37441 tggccacgac gatcatgatc gaaccatcag cactgctgtc attgagtttg tcttttaaat
    37501 aatattggcc gaggttttta ccaacgggga tgccgtccat ttgcagcaca ccgccaaaat
    37561 ttgattgcac caacacacct actgtatgcc cgccaaggtt ttgaggtaac acccgcgaac
    37621 tcgtgcccat gccgcccttc cacccaaaac agatcgtccc cgtacccgca ccgacgttgc
    37681 cctcaaaaac ttgtccatct tttgcgcttt cgattgcctg aatgacgtgt tccgttgtaa
    37741 ttgccagcct gcggatgtta ttcaacgtcc catcattggt ctcgcccaca ataggattga
    37801 cgcttcgcac ttcaccgtta cctgcctgcc ccaacgtaaa caggatcagc gcctcagccg
    37861 cgcgcggcac agacagcgtg ttggtcaaaa tgatgggtgt ttcgatctcg ccaagttcca
    37921 tgatttgcgt ggaccccatc aacttgccga aaccatttcc caccgccacc cccgcaggga
    37981 ctttctcttg aaacagattc ccagcatgcg gcaggattgc agtcacacct gtgcgaatat
    38041 ctgtcccttc aatgagggtg acttgtccca ctttcacgcc tgctacatcg gtgatggcgt
    38101 tcagctttcc cgttggcaga atgcctgggg tgatccccaa ttcccgtgcg cgtttgcggt
    38161 tttccattct aaaccttctt cacgatcaat gtgatttgat acacaccttt gcctcggtct
    38221 gccagtgtgg cggggtaggg cagtacggcc atcacccgaa ttgaatatcc ttcgatttcc
    38281 acatggcttt ggatcccttc gaattccatt tcgggtgcgc ggccttgtgc attggtaaaa
    38341 gtttgcaaat tgacttcatg ccgcccgccc tggtctgttg tgacgatcag cgtgagtgac
    38401 acaggtccgc tcatggcgca ttcgatctgg cttgggcagc gggcatcatc cgtcacgccg
    38461 accagtttca gggtcagggg tgaatcgttg atcgtggctg attgtcccgc aacgaggacg
    38521 aattcctcat ctgtatttac ggtcaggtct ggcgggggag tcgggattgc ggttgcagtt
    38581 ggcggctggg gttgcagggt ttggcaggcc agtacaaata ccaacaaagc catcagagcc
    38641 acaggtttga gtttggacat gggaacctcc attgagtatg gcgattatat atcgccgatt
    38701 ttgtcaggga cggatggcca ggcacacagg ttccagaatc agtaccagag tacctgttga
    38761 cctagtggaa aatcactagt attatttact tatcaatagt aaaaacctga tactacaaag
    38821 aggacgttat gcaattgaac attcaacatc ttggcaaaca atataaacgt gatttctggg
    38881 gcttgaaaga cttctcgctg gagatcaaac caggcatttt aggcctgctc ggcccgaacg
    38941 gtgcgggcaa atccaccttt atgcggatgc tggcaaccat taccaagccg accgaaggca
    39001 gcattaaatg gaacgaggtg gatgtcgtca aacaacctga tgcattgcgc gatgtgcttg
    39061 gatatttgcc gcaggatttc ggcgtgtacc caaatttgac cgcagtggaa ttccttgaat
    39121 acatggccgc cattaagggg cttgatgcgg tcactgcgaa gaaacgcatt gatgaattgc
    39181 tgcaattgac caaccttgtg caggccgcga aacgtccgct gggcggatat tcgggcggta
    39241 tgaaacagcg cgtgggaatc gcacaggcct tgctcaatga tccgcaattg ctgattgtgg
    39301 atgaacccac tgtggggttg gatcctgaag agagggtccg cttcaggaat ttgctctcag
    39361 acctgtcggg agaaaggatc gtgatcctgt ccacgcacat cgtttcagat gtggaagcga
    39421 ccgccacaca catcgcactc gtcaacaaag gtcaactgct gcgtgaggct gcgcctgaaa
    39481 aactgttgca ggagcttgaa ggcaaggttt gggagtggac gattcacagc gatgatctgc
    39541 ccgcattgaa acaaaagcac atcatcagcg gtacgatccg cagaagcgac ggggtgcagg
    39601 tgcgtgttgt aagcagcacc cagcccacag cggagtctag aaacgcgagt ccgaatcttg
    39661 aagacgcata tttgtttttc atcggcggta aacaatgaaa tcagcacgga tcatttatca
    39721 acttgcccgc gccgattttc tggagcgtgt gcgccgttat agttttcttg tgatgttggg
    39781 gctggtggtt tttctcggtt atcaaaccgc catcggcaat atgtctcttg agttgggagc
    39841 gtatcgcggt gagttcaatt cggcctgggt cggcgcgatg atgtcgctga tcggtacatt
    39901 tttcatcggc tggtttggct tctatctcgt caaaggctcg gtggcccgtg accgcgaaac
    39961 gggcgtcggc cagatcatgg cgaccacacc gctcacgcgt ccgctgtatc tgcttggtaa
    40021 atggcttagc aacttcgccg tgttgatgtc gatggttggt gtgctggcgc tggctggcat
    40081 cgccattcaa ctgctgcaag gtgaaaatac gcagatccaa ttgctggaat ttcttgaccc
    40141 atttgttttt atcgtcatgc cgctcatggc gctggtggct gcgctggccg tgttattcga
    40201 gtcgatctcg ttcttgcaag gcggctttgg caacatcgtt tactttttcg cattcatcat
    40261 gttcctgccg ctcttcatgg aaaacgacac gttgcaaaaa taccccgcct tcgaaccgat
    40321 gggccttggc ctgctggcag gcgaaatggg tgatgctgtc cgtgccattc atcccgatta
    40381 cgatggcagt ttcactctcg gcggcggcat tgtggaacag gccaccacca cctttgtctg
    40441 gaatggcatt gcctggaccc ccgaatatct ttttgcgcgt ttcgctttga tcttctttgc
    40501 cgttggcttg attctctttg ctgcgctttt cttcgaccgc ttcgaccctt ctcgctcgaa
    40561 accgcgcaaa gcgcggtccg cttcatcctc cgagccgaca cctgcttcaa cacctcaggc
    40621 attccctgcg ccgcatctga ctccgctcaa caagtcggcg aaccgcttca gctttttcac
    40681 cgttctcatc gcagaactta aactcctgat caaaggtcaa cgctggtggt ggtacgcaat
    40741 ttccctggga atcatcgttg cttgttttgt gagcccatcc aaagtcgtgc gtgagatcgc
    40801 cctacccatc gcatggatct ggcccatttt gatctggtct tccattggca accgcgaggt
    40861 gtacaacaat gtccagcagt tgaccttctc ttcggcctcc ccgttgtggc ggcaggtccc
    40921 tgctcaatgg ctggctgggt ggatcgttac cctgcttgtc agcattggag ccatctcccg
    40981 cttcctcatg gatggagata ccgtcggttt gcttgccctg ctttcaggca cgatcttcat
    41041 tccatccctt gctcttgcct caggcgtgtg gagcggaacg agcaaactct tcgaaattct
    41101 ctacatggtc atctggtatc ttggcccgct taacaacgcc cccggcctgg actttatcgg
    41161 ttcacatggc aatggccgtc ctgaattttt cattccgctt tcaattgcgc tggttgcctt
    41221 tgccttcttc ggcagggcaa ggcagttgca gcaataatgc agtcccttat ttcggcggct
    41281 tcattggcat cagaaccccg atctttttgc ccccatcaac caacactatc ggctcaagcg
    41341 gagcattgac atggaaggac aatttctgct tgtaacgcag cgaggtcagg tatttgatgt
    41401 atttgccgtt gagcagcacg tcaaagggcg cgtagtaatt tccacgcgct gggtcttcca
    41461 gtaaatgctc aattccgaaa ttcgatttga cattccagtt caacaagcgt tgaaaggttt
    41521 caacaggcag agccttgccg ggcaaaggct ctgccgttcc tgtgtaggat gcagccagca
    41581 agatttggcc gtcacttacg aagcgtgttc catcaggcag gacacaatga gctgcccact
    41641 ggattggggt tgacacgggc ctgtcggtga gcagggttac ggtatctgca agttcaagga
    41701 tgctggcaca cttggcacac tggacgaact gcgccccggc accgatattg gaaagttcgt
    41761 ttgtacaatt gggacaggta tgcatgagga cgctcctttt gtgggtgggg aagtaagtgg
    41821 ggcgaatgtt ttcctatttc caaatatgcc cgtacccata ttcgccttcg atcaacttcc
    41881 cttcagcaaa tcgttgaaag cggaacgggg tgatatccac agtttttggc tttccatcca
    41941 gaatcaattc caccatgctt gcgcccgcgg cgggggaggt cttgaagcct gtgccgctca
    42001 acccgcaggc gagataaaaa ccatcaggtc ctgctgcgct gtaaatggca cgctgatcgg
    42061 gggtgatgcc atcgcgcccc acatgaatgg aatgtaaccc gctctcttca attttcggca
    42121 tccgccgcac cattgcatcg atgagttttt ccacaaaagt gggtgtggga gtctgatctt
    42181 cggcatcggg tgcttcgccg cgcagagatt catcctcacc cgcagcaagg atcagcgcac
    42241 tgccctcagg acggaaatag caattgatca catcgtcaat caccgcaggg aatttaccca
    42301 atgaaggcgg gcgatgcagg aatgccacat catgagtcca tgtcaccagc ggcacatcca
    42361 cgcctgccag cgccgcgacc tgcttcgccc acggccccgc cgcattgacg atgatcggcg
    42421 cagcgaaatc gcctttcgta gtgctgactc ccgtcatttt tccgcctgct gtatggatgg
    42481 ctgtcacttc gcagttttga atcagggccg cgccgtttcg tttcgcagaa tcaagaaacg
    42541 agttggtcgt gagcgtcgcg tcggcgtatc ctgaatcggg ttcgtaggcg gcgaaatcaa
    42601 agtgttctgt cacgagatcg gggaaaagtt tcttcacttc atccgctgaa atcaccgatg
    42661 tgttgattcc aattttctgc tggttggcaa cattgccgcg cagtttatct tcatgttcac
    42721 gtttggcaac ctgtaaaaag ccagtcttct gaaacccaca ttccccgccc acacgttcac
    42781 gccaattggc gaaataattt ttataactgg tgaaagtcag ttctgattcc gcggcgagat
    42841 cgtaatgcat acgcaccaat ccactggagt gaccagtggc gcccgaagca gtgactttgc
    42901 gttcaaggat ggcggtcttg agtccgcgct cggcgaggtg gaaggcaatg cttgcgccca
    42961 ttaccccggc gccgataaca atggcatcat aggtctgtgt catgtgaggt tccttttgca
    43021 ttgggattgc cgcccattat ataacctttg gctcgggatg tctggcgcga taatttatga
    43081 caaacattac attcgtaaac ctgaatgtgt tgctacaata tattcaggtt tatgaatatg
    43141 aaaaaacaat ccatttctca aatttccgaa cagatcgccg cgccgctggc tgccatttcc
    43201 acaccacagc gcattgccat tctgcttgcc attggcaccg gcgaagcctg cgtctgtcat
    43261 cttgaaaccg cgctgggatg gcggcaggct tacatttccc agcatctgat ggcccttcgc
    43321 aaagccgata ttttgcagga tagacgcgaa ggccgttacg ttttttataa attaagaaac
    43381 gcctcttttc tggacctgat cactgcttca gcaaccctaa gcggactcgc cgccgaggca
    43441 gtttccacgc tcgtcaatac ccgcacgtat ccagcatgtg aatgcccgca atgtgtccct
    43501 gcgttaatcc cagcaaatag tttgtaagga aacaatgacc actctttcat ccccatcgca
    43561 atctcacaag tatctcccgt cccttgtgtt tttctctgct gtgatctggt tgatcgccta
    43621 caacaccatt caacccctcg cagattggat ctcttacaca gccctcggac tggcgcgcga
    43681 ttcgcgcctt ggcgaatcac tggctttctt tttgtacgat gtgccaaaga ttttgctcct
    43741 gctttcgggc atggtcttcc tcatttcaat tgtccgcact tttttcagcc ccgaacgcac
    43801 ccgcgccctg cttggcggaa aacgtgaagg ggttggcaac atctttgccg ccatgctggg
    43861 cattgttacg cctttttgtt catgctctgc ggtgccgctt tttattgggt ttgttgaatc
    43921 aggcatcccg ttgggtgtga ccttttcctt cctcatcgct gcacccacga tcaatgaagt
    43981 cgctgtgatc ttgttattcg gtctctttgg ttggaaggtg gctgggttgt atgttgcatc
    44041 tgggcttgtc gttgccattg tcgctggctt catcatcggg cggctcaaaa tggaaagata
    44101 tgtcgccgat tttgtctgga aggtccagcg caaagtggat gcctttgaag aaaagttgac
    44161 ctgggaagat cgcatcgaac gcgcctggga ctcggtgaag gaaattgtcg gcaaggtctg
    44221 gttatatgta gtggttggca tcgccatcgg tgcagggatt catggctacg tgcccgaaga
    44281 tgcgatggct tccattttgg gcaaacaggc ctggtggtcg gtgcccgtgg cggttctaat
    44341 cggcatcccg ttgtattcca acgcggcggg tattgtcccc attgtgggca ccttgatcga
    44401 aaaaggcgca tcggttggga ctgcgctggc gttcatgatg tcggtcatcg ggttgagcct
    44461 gcctgaagcc gtgatcctaa agcgcgtgct caaaatgccg ttgcttgtga cgttcttcgc
    44521 tgtgatcgca gtggcgatca tgatcacggg atatatgttc aatttgattt tgtaaatgac
    44581 aggccccagg gtttctgaaa acctctgggg tctttatata aaggagcaaa aatgttaacc
    44641 atcaaaatcc taggttcggg ctgtgagaat tgcaaacgcc tcgcatggct tgccgaccgt
    44701 gtggtcaacc acatgggtgt gcaggcgcag attgtcaaag tcactgacta tgccgagatc
    44761 atgcagtatc ccatcatgtc caccccgggg ctggtcatca acgaaaaatt ggtctcttcg
    44821 gggcgtatcc ccagcgaaac agagatctcc gtttttctga cgaccgctct tgaaaccgca
    44881 taaatgtttc ttgcaaattt aatatggagg caaaatgaaa aacgaaacgc gcaaagaact
    44941 ttcctttctt gaccgctatt tgaccttgtg gatattcctt gccatggcag tcggtgtagg
    45001 catcggcttt ctcttcccga aagccgtcgc ggacttcaac gcggcggttt ccattggcac
    45061 gaccaatatc ccgatcgcga tcggactgat cctgatgatg tacccgccgc tggcgaaggt
    45121 gcgctatgac gaattgggcg atgttttccg taactggaaa attctcagcc tttcactcgt
    45181 gcagaactgg gtgatcggtc ccgttttgat gtttgtactg gcgatcgttt tcttgcgtgg
    45241 ttatcccgag tatatgacgg gtctcattct cattggcctt gcgcggtgta ttgccatggt
    45301 cattgtgtgg aacgaactgg caaaaggcga taacgaatat gcagcgggac tggttgcatt
    45361 caatagcatc tttcaggttt tgttctacag cctgtacgcc tacgtattca tcaccatcct
    45421 gccgacatgg ttcggcatga caggcaacat cgtgaacatt accatcggtg agattgccag
    45481 aagtgtgttc atctatctgg gtattccatt catcgcaggc tttttcaccc gcttcttcct
    45541 tgtccgcgcg aagggcaagg attggtatca caaggtcttt attcccaaga tcagcccgtt
    45601 gaccctggtg gcgttgctct tcaccattgt ggtcatgttt tcgcttaagg gtgacattat
    45661 tgtgcagttg ccgttggacg tggtcagaat cgccattcct ttgctgatct actttgtgat
    45721 catgttcctg gtcagtttct tcatgggtaa atccatcggc gcggattatt cgcggacaac
    45781 cacactttcg ttcactgccg ccagcaacaa ctttgaactg gcaatcgcag tggctgtggc
    45841 ggtctttgga atcaactccg gcgcagcgtt tgcagcggtc attggcccat tgatcgaggt
    45901 gcctgtgatg atcgccctgg taaatgtggc cttcttcttt caaaagagat attttggcgg
    45961 cgtggtgagt tctgccccag ccagatagac gatatccccg acataaaaaa acagtggtca
    46021 accttgaatt gggttgacca ctgtttttat gtgggctcat ttgtaaagtg acgtttgagt
    46081 ttcagcaatg ggtcttcgag aatgttccag gttagtaatg ccaatacggt tgtgatggca
    46141 actgtcagca ggctgtaggc ggcgtagtat tcccatccgc gtactttggc tttgaccatg
    46201 atgtcgagca gggtcagggc cacgggcatg tggaagaggt acatcgagta gctgtatttg
    46261 ccgaagaaga ccagcccgcc attggcgaaa aattttcgaa tcaacgaagt ctctgcgccg
    46321 ctggtgagga aaacgatcat ggcggcggag gaaaatgcca gcagggtgta tccaatggtg
    46381 ccgaggggaa tattcctgta taacggatgc ggccctgcgg ggctggcaat gaacgccaga
    46441 tacaggaaca ggatcatcgt tatgatggag acgggcaatg caaatcgttt ggctgtctcc
    46501 tgccagtttg tgggtgtgac gaagaagatg gcggttaatg cgccaagggc caggccctca
    46561 aaacgggtga aggtgttgta gaaaaagaca acaggcaatt gctccgaatc tggcgagagc
    46621 aggatggccg ccgtgcgaat cagcagggag aggatgacaa tgcctgcgct gatccatgtc
    46681 aatgtgtgtt tgttgacaat gcgtacgatg gcgggccaga tcaaatagaa ctgttcttcg
    46741 attgccagtg accaggtaac actcaaatag gcggaaggat agatcgcgaa caggtacagc
    46801 cagttctgct gatagagcag gaaggtcggc aaataaggga tgaacgaatc ggcggtctct
    46861 gccacagcgg ggacgaattt cactgcgatg aggaaaagcg caagagccgt gtagtacaac
    46921 ggaaaaatgc gcagcacccg ccgcgcatag aagttcttgt aataattttt ttcctccctt
    46981 gtcttcagca ggatggacgt gatcagaaag ccagagagta cgaagaaaat atccacgccc
    47041 atccagccga ccagtgacag gcgtgtgatg aaatgtaaaa acccattggc ggtgaagtaa
    47101 tcagcccgtt ttagaacgtg gtgcgcaatg acgagcagga tcgcgatacc ccgcaatcca
    47161 tccaattcct ttgagcggtt gttcatgatg cggcgatcag ctcatcatat tttttgcgca
    47221 cttcgcgaat gtcttcccac atttgggctt tgggcgaacc aggttccttc gagggattcc
    47281 tcaacaagta ggaggggtga aagatcggca tgatccagcg gccctgtgat tctgtccact
    47341 tgccgcgcag ggaggtgatt ccagtcttgc gcaggatgga atagctggct acgttcccgg
    47401 tcaacagcat cacaagcgga tcgatcagtc ggatgatctc gaaaacataa ggacggtaaa
    47461 aatcggtttc ggcatcggtc ggtttgcgga aggtcttgcc ttcctcaccg ggcggcatgc
    47521 ggaaaacgga gttggtaatg tacacatcct tttcggggtc gaagtctgct gcttgcagga
    47581 tcttgtcgag catctggcct gcacgcccta caaaaggttt gccctgaata ttttcctcgg
    47641 ggccgggtgc ttcaccgatg atcaataatc gggctttcgg gttgccacgg ctgataacga
    47701 tattggtgcc agcctttgcc agcggatcgt cgttcatgcg tttcatcgcg ctgaataatt
    47761 cttcgagcga tgaatattgt ttggcgctag gggtgggttg ggtcatggtc tgctcctcgc
    47821 aaggggatga gttaatttga ttctacccta tgaggcatct cataacgtgc tcttttcttt
    47881 tacttctttt ggtgtttgta gtgaacatct gacaatgaac gcaatcgttc tgtcgc
//
LOCUS       DAOQLC010000014        47323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_206_length_47323_cov_23.669353, whole genome shotgun sequence.
ACCESSION   DAOQLC010000014 DAOQLC010000000
VERSION     DAOQLC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47323
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206_length_47323_cov_23.669353"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PL000_04110"
     CDS             complement(<1..511)
                     /locus_tag="PL000_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC88109.1"
                     /translation="MNVSEAIRLKRAVRKFQDKPLPDDVVHAILNAGRRSQSSKNNQA
                     WRFIAVREKETLKALSQCGEWAGHIAGAALCVAILTPEPTEKFQTMFDAGQAAAFMQL
                     AAWELGIGSCPASLYDFERTRQILTFPPEWHLRIALSFGYALDEEKISAAPKKGGRVS
                     LDEIVHWEKW"
     gene            668..2824
                     /locus_tag="PL000_04115"
     CDS             668..2824
                     /locus_tag="PL000_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNC88110.1"
                     /translation="MTLPTVSETTLLKEFAYTLRPVERGYANRTLYINLNDNTILEKP
                     VTQQMKDLFTGGRGFALKLLWDAVTPETKWDDPQNELVIANGPICGITAYPGSGKSTV
                     VTLSPLTENVIDSNVGGYFAPFLKFSGFDALEIQGKAAKDVIIFIDGDTGRVTIEADS
                     LEAVDSHQIGNVLTERFGGDEKGKRAISVLSAGQAAEHTRIAMINSTWYDVRRKEARF
                     KQAGRGGPGRVFRDKKIKAIVVRYSDMGGDKNGVADMPLIRKAGQRINKEIADLDDKQ
                     NQMRKVGTANIVEVMDHFDLLPTHNFRYGSHPDTHKIDSKVWKEKFTQGLPDGCWLGC
                     TMSCSHGVDHFPLQTGPYAGDCVLVDGPEYENAAGLGSNIGNFDPQKVLELNFYCDTY
                     GIDSISFANCVAFAMECYEEGIITKEQTGGLELKFGNGSAALDLIHQMARGEGFGVIV
                     GQGVRYMKELFIRQYGADPKFIHDIGMEIKGMEISEYLTKESLAQQGGYGMATKGPQH
                     DEAWLIFMDQVQNLLPSFKDKAEALHYFPMWRTWFSLHGLCKLPWNDISPENNKQTKE
                     PAKVEEHVENYTWIHQGVTGKPTVAEDLLHQSERVYNFQKVFALRMGRVGRKHDYPPY
                     RAMGPVTAAEYESRQDRYDEQLKRLVEVDPANLTTQEKMAHLRKYREEQYEQLMDAVY
                     QRRGWDANSIPTLEKLHQLGIDLPEVVEVVKQAQKK"
     gene            2989..3387
                     /locus_tag="PL000_04120"
     CDS             2989..3387
                     /locus_tag="PL000_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88111.1"
                     /translation="MDSQITFRQYLFSTLALLLIGWGGLALLLIYSVPFVWARWGFYV
                     CGIMALTGTALPVVYFLHRRFPTTPQAEPGVIVRQAMWVGVYGATLAWLQLGRLVTLY
                     VILGLAGGLIAIEYFIRLREKANRRLPDIQ"
     gene            3384..>4738
                     /gene="selA"
                     /locus_tag="PL000_04125"
     CDS             3384..>4738
                     /gene="selA"
                     /locus_tag="PL000_04125"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNC88112.1"
                     /translation="MTTLRDLPSIDQILQTHLAAQLIARYGRPLTLSAIRFTLDEIRA
                     RFKSGQITALPLRDLILAQADATLTAWTKPTLIPVINASGVILHTNLGRAPLSDDTIR
                     AMDVASRGYSNLEYDLEKGARGSRLIHAESVLQRLTGAEAAMVVNNNASAVLLVLSAL
                     ANKKRVIISRSQLVEIGGGFRIPDVMKQSGARLVEVGTTNKVHLRDYEEALLGANAIV
                     MRAHPSNFKIIGFTEEPELKDVVALAHAADTILVDDLGSGTFVDTARFGLSHEPTVQE
                     SLAAGADIVCFSGDKLLGGPQAGIIIGKRDLIEKIRKHPLARAVRADKTCLAGITATL
                     LHYLKDEAEREIPIWKMISLTPKQVKGRAEAWAANLGQGTVVEGESTVGGGSLPTESF
                     PTFLLSLSVSSADKFLKKLREQNPPIIARTADNKVLLDPRTVLPEQEGALLVGLKNVM
                     K"
     assembly_gap    4739..4838
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4943..5383
                     /locus_tag="PL000_04130"
     CDS             4943..5383
                     /locus_tag="PL000_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNC88113.1"
                     /translation="MDEIYIVEKELSYLIMQVAFEVHNTLGPGFPEVIYEEAMNRELA
                     RHGVNLERQKHIKVFYKNEPIGEFVLDNVANGRVILEYKAVAEIAKIHVQQALSYLKA
                     TGLELAIVINFGSTRVQSSRVVFKNGKSNFPSQTPRSRNVLRND"
     gene            5446..6699
                     /locus_tag="PL000_04135"
     CDS             5446..6699
                     /locus_tag="PL000_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNC88114.1"
                     /translation="MKSELDTLMQAKDMDAILIFGNAEHNPPMYYLTGGGHVSHATLI
                     KKRGGDATLFFGDMERDEAAKSGLKLIPYSTYDFEEIYKKANRNPTLAGAMRLELMLK
                     DVGVESGRVGVYGTYDIGPVFGLLMQLQKLMPNLEFIGEPREDSIFLRAMEKKDEAEV
                     ERIRKMGKVTTTVVGRVADFLTSCEVREDEVLLNENGSALTVGDVHSKIRLWVSELGA
                     ELPSGFIFAIGRDAGVPHSAGNPADLMKLGQTIVFDIYPAEAGGGYYYDFTRTWSLGY
                     ATPEAQELFDQVKEIYDQLNENFDLNVPFKDYQKMTCEYFGGKGHKTPMNSKSPVDGY
                     VHSLGHGVGLNIHERPFSGINTSDEERLAPGVVITSEPGLYYPEKGMGFRIEDTIWVR
                     PDGRMEILADYPYDFVLPMKKWKKK"
     gene            6699..7724
                     /gene="tsaD"
                     /locus_tag="PL000_04140"
     CDS             6699..7724
                     /gene="tsaD"
                     /locus_tag="PL000_04140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC88115.1"
                     /translation="MDLKPARILALETSCDETACAIVENGRALLSSVVASQMEIHARY
                     GGVYPEVASRQHVLSVTPVVEQALAQAHLTLKDIDALAVTQGPGLAGSLVVGMNMAKG
                     LALGSGLPLIGVNHLEGHLYSAWVHDSGELVPPAPQFPLMALLVSGGHTELNLMTDHL
                     TYQRLGSTLDDAAGEAFDKVSRLLELGYPGGPAIQKSAEEGDPNRFHFPRAWLEGTWD
                     FSFSGLKTAVLYEIRELQKRTHTLPVPDLAASFQKAVVDVLFKKTINAAREFGAKEIL
                     VAGGVSANRMLRQTFRAQTEFPVHIPPLSLCTDNAAMIASAGYHRYALGHLSQMDMDV
                     LPTWPLP"
     gene            complement(8015..8632)
                     /locus_tag="PL000_04145"
     CDS             complement(8015..8632)
                     /locus_tag="PL000_04145"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC88116.1"
                     /translation="MTTKIISTSKSHSTEDALLTLQQGGLVAFPTDTVYGLGALAFDN
                     AAIENIYIAKNRPIEKAIPILIGNMDMLHLVTDHIPDMAYTLMARFWPGPLTCVLPKK
                     QTLPPAVSATSTVAVRIPNHPDALTLLTAAGPMAVTSANMSGHPSPTTAQEVYTQLHD
                     RIPLILNGGQTPGGIPSTLVDCTGETPVILREGPITLDELLNALR"
     gene            complement(8604..9785)
                     /locus_tag="PL000_04150"
     CDS             complement(8604..9785)
                     /locus_tag="PL000_04150"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNC88117.1"
                     /translation="MNRYNGIVPKLNSLYYQVTDTDAVEPSEQRRGRLMAMYLIMMVV
                     ILVHVTISNLAYLIIAKEGMYFNYIIQDVIVLSVLFLFWHLNRRGYTKLAAHACINIS
                     IFGAVLLYEPHDLEFTMVILALPIITSSFVLHPIASFFYALLTTLGYTYSSYYYDSLI
                     SYNFTGVLALFAIAFFTFFTAWQLNHTLNKNQFLITDLRSSYETTLDSWSRALDLRDK
                     ETEGHTQRVTKLTYRIAKAMRLTEEELLHIRRGSLLHDIGKLGVPDEILHKNGPLTDL
                     ELKVMRQHPQFAYDLLYPIEYLRPAMDIPYSHHERWDGSGYPRGLKGLEIPLSARIFA
                     VADVYDALTSDRPYRKAWPPEKVLEYIKNESGAHFDPNIVEIFLHEMKNLNDNKDHLN
                     Q"
     gene            complement(9942..10790)
                     /gene="prmC"
                     /locus_tag="PL000_04155"
     CDS             complement(9942..10790)
                     /gene="prmC"
                     /locus_tag="PL000_04155"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNC88118.1"
                     /translation="MNAGEYLNNHKAALDGLDAQILLAHVIKRPRTWLLAHLETPLTP
                     PQLVEAEQAFARLIAGEPLPYIIGHWEFFGLDFDITHDVLIPRPETELLVEKALAWLN
                     RHPEARSVADIGTGSGAIAVSIAANIPDAHILATDLSPAALNVAKGNAEKFHVQNQIQ
                     FLECDLLPASSPNLHSSSFNLLCANLPYIPTSTLRQLPIFGREPTLALDGGPDGLDVY
                     RRLFKAAPAWLKPGGMMLFEIESTLGVQAAGLAFDSFSNAVIHLHQDLAGKDRLLEII
                     PSPDAN"
     gene            complement(10839..11915)
                     /gene="prfA"
                     /locus_tag="PL000_04160"
     CDS             complement(10839..11915)
                     /gene="prfA"
                     /locus_tag="PL000_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC88119.1"
                     /translation="MLDKLQVIEERYEQLGSELLEVGSDYQRAGEINKERVELEPLYN
                     KIREYRQALKSLEEAKSIIISENDSELIALAKADVDDLEPKIEALEKEIKGMLVPKDP
                     RDERNVIFEIRAGAGGDEAAIFAADLFRMYTRYVDGKRWKVELMSESAIGVGGYKEVI
                     FEVKGKGAYSKLKYESGVHRVQRVPATESQGRIHTSTATVTVLAEVDDVEVDIPESDI
                     EIEVYRSSGAGGQNVQKNSTAVRLYHKPTGMIVTCQDERSQLQNKLRALSILRARLYE
                     IEEQKRRAELEADRRSQVGSGDRSEKIRTYNYPQSRVTDHRVGFSSYNLSAVMDGAID
                     QFIDELSTRDESERLAATGVDDDE"
     gene            complement(12057..12203)
                     /locus_tag="PL000_04165"
     CDS             complement(12057..12203)
                     /locus_tag="PL000_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88120.1"
                     /translation="MAGKTSKTKKQGKPADQGNSNQRAAQILFAVFAVILILSMVLSA
                     VANY"
     gene            complement(12212..12592)
                     /locus_tag="PL000_04170"
     CDS             complement(12212..12592)
                     /locus_tag="PL000_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88121.1"
                     /translation="MKQVWIVDDDEEMGHAISLMLKLLDCETKHFINPRPAAQALLAG
                     GRPDLFVLDINMPEVSGLDMLEFLRRRKEWKDLPVIMLSSEAADVMVDKALEMGADGY
                     VMKPVTIDELERVMAQAFYRHITK"
     gene            complement(12589..14466)
                     /gene="uvrC"
                     /locus_tag="PL000_04175"
     CDS             complement(12589..14466)
                     /gene="uvrC"
                     /locus_tag="PL000_04175"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC88122.1"
                     /translation="MEISEKLQGILATLPSKPGCYLYRNVEGKIIYVGKAINLKNRVR
                     SYFHADSSHDNKTRRLVRDIVDIEWIVVGSELEALILEMNLIKKHRPKYNIRLKDDKR
                     YPYIKVHWHEPFPRVTVTRNMDEDGSRYFGPYTSAWAVYQTLDVLRKIFPYLTCDREI
                     TGLDKRACLYFDIKLCLAPCIGAVDQAGYRQMISDLMDFLSGHSEEIIKRLEAEMLKA
                     SEEMRFEKAAAVRDQLKAISSIIERQKIIFATDYKDSDVLAMARSDGEACVQIFFIRG
                     GKLIGREYFILEGTEDTADTEVMAEFVKQFYTEAATIPDQVMIPEEIQVEEAKIIGQW
                     LRSKRGGSKVELFVPKEGQPHELVQMAAENASETLQALRAQWQADAHKQEQALSELQN
                     ALKLSAPPNRIECYDVSHTQGVATVGAMIVFEQGVPAKNLYRKFNIDSTSIGAPDDFA
                     SMEEMLTRRFKRWLSSQETEAGPGSKKDASFSFLPDLIIIDGGKGQLGRVVRVLEEFE
                     LYDKVPVVGLAKQEEEIFFPHNSKPLLLPRHSQGLYLVQRIRDEAHRYGITAHRARRS
                     KQGLASQLDSIPGIGPARRKALLKHFGSLDKIKEASVTDLAAVKGMNETAAESIKAHL
                     E"
     gene            14665..14970
                     /locus_tag="PL000_04180"
     CDS             14665..14970
                     /locus_tag="PL000_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88123.1"
                     /translation="MEPSFSNAPIQNGPTGASGGNEKIMAIASLVIGVINLCAWLLPI
                     CGIPLALVGVVLGFLGMKDESQKMLAIAGIGLSAFSLLLACGNAIYGVYIATQGMSF"
     gene            complement(15026..17137)
                     /locus_tag="PL000_04185"
     CDS             complement(15026..17137)
                     /locus_tag="PL000_04185"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC88124.1"
                     /translation="MPSWNGKTLGKVQVGELIARGGMAEVYLGEHTTLNRKVAVKIMR
                     DHVDSDPDNHVRFEREARVVAGLRHPNIIQVYDYELVDGQPCLIMELVTGASMGNYLK
                     ALHKRGEKLPLETVALILSKLSSAVDYAHNQNIIHRDIKPANVLLRSPSSPIDLNKPL
                     PADVEPILTDFGLVRLLDSSIQTSTGTVSGTPAYMSPEQARGEKVNSKTDIYSLGVML
                     YEMLAGVVPFDAESSFGILMKHLNDPPPPIFGISSDLQAIINRALSKDPELRYDSAKA
                     MADEFLAVFNGQTISADTARIAKIPQRVVKPAAEAAAEGKKSTRNNPTLMWVGVALGV
                     VAITALGLVFFKPQTPQATPAPEATLPPPTPTVDPNEILGRVVFTDQNGLMDKAALSF
                     DDLPIPEEGTHYDVWFLGQGGEFVRKAGSITKESLAQGQLVYTNPEQNNVLSLFDQIE
                     ITLEVDNDPNPDTSSGNVVASSVFPPMSLIHVRHVVVSFVTAPEGVALIQGLWGATES
                     LNTTTTELGEAYKAGDETLTRQKLEEIANLIAGNANTVQYIDWDGDGVVSDSGDGFGL
                     LQNGEPGYTDQGYIAQSISHAQFAARAPDSTANIKLQSANVVICLQNMQGWTEQVLEK
                     VLRLQEMPFGPEMEPIITEILTLSDQIISGVDSNNNQLIEPIVGEGGATTAYEYAYYM
                     AEMPLLLGIHRVPPPAPTPGP"
     gene            17188..17673
                     /locus_tag="PL000_04190"
     CDS             17188..17673
                     /locus_tag="PL000_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNC88125.1"
                     /translation="MTHALEETLREHLRTQGLTVVTAESCTGGLVSDRITNISGSSEY
                     FPGGIVAYSYEAKASLLGVSWDTLNTHGAVSEETVLEMARGVRKLFKSNIGISVSGIA
                     GPTGGTPEKPVGLTWFGLSTDKGEWARHFIWDGDRLQNKHHSSEAALQFVLDYLDGKL
                     E"
     gene            17670..18350
                     /locus_tag="PL000_04195"
     CDS             17670..18350
                     /locus_tag="PL000_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNC88126.1"
                     /translation="MKAVVIVSANAEWRVVKELYPNLEIKNSPYGEYAHLTLGTWNLE
                     LFHGGWGKISAAATAQYVIDHFQPDLLINLGTCGGFAGRIETGTIILVERTLVYDIIE
                     QMGDGDEAIEFYASSLDLNWLPSNLPDSTRRGLLVSADRDIVVEDIPMLIKKYDAVAA
                     DWESGAIAWVAQRNNLRVLILRGVTDLVGGDGGEAYGNLGLFHERTKSVMGELFRQLP
                     DYLNLIKI"
     gene            complement(18517..19800)
                     /locus_tag="PL000_04200"
     CDS             complement(18517..19800)
                     /locus_tag="PL000_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNC88127.1"
                     /translation="MKSITFQIIALCVMLSMILAACTPSVSLSVAESNKSRDKNPQIA
                     EGDLSTLVDGNNAFALDIYNALRSENGNLILSPYSMSLALAMTYAGARGDTESQMAQA
                     MHYQPQAQLHPAFNALDLALEKKPINLDKDQEPLQINIANSVWAEKTMPILPDYLDTI
                     AVNYGAGIHLADFLNKPNEERVAINNWVSAETEDRIKDLLAEGSITPDTRMVLVNAIY
                     FKADWLHKFDANSSYDSTFHLLDGTEATVKMMGENIHDIPYMQGDGYQVVELPYAGET
                     AAMDIILPEEGNFEAFEASFNKDVYDEIVNGLQVVPSARVNLPKFEFTKDFSLSDALI
                     KLGMVNAFDRGLADFSGMTGEKDLFIGDVIHKAFVAVDEEGTEAAAATAVVMEATGAP
                     VQDIIFIADRPFLFVIRDTVNGQILFVGRVLNPAQ"
     gene            complement(19851..20318)
                     /locus_tag="PL000_04205"
     CDS             complement(19851..20318)
                     /locus_tag="PL000_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88128.1"
                     /translation="MQTQHIKDAKMVITAVAQRIYMPEKTVQYFWDILEAEGELSDVD
                     DFQHAYHENHGLFLVVLDEEKVVGTGAVKKINADTAELKRLWLLEEYHGQRIGFQVVM
                     RLMEFARAQGYRRLRLQTSHKQIRAIHFYKRLGFYEIPSYRESMDNVSMEIML"
     gene            20397..21623
                     /locus_tag="PL000_04210"
     CDS             20397..21623
                     /locus_tag="PL000_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88129.1"
                     /translation="MMNTPEQKEIQKRNFRYVQIDAVGVSIANVAAPFLPVFLTRLGA
                     SNFQVGLLTSMPGITGLVLAILVGRFLQTRPNIVPWYSVSRLLVISCYALTGLLTFVL
                     SKEYAVISTLAIWAFATLPQTALAVAFSVVMNAVAGPEGRYALLSRRWAIFGITTVVM
                     TFFVTRVIDLVEFPRNYEMMFLGLSVGGFISYYFSKQIKLPDQTPPTPAPSSSVFDSA
                     RNYFSLIRGEPAFVSFAFKKFVYVSAITLSQPILPLFLVREVKATDSQIGTITMAMSL
                     VMLVGYFIWPWSSRKYGGRFVLLATTFGMILYPALTAATPQVPLIIIYAGITGLFQAG
                     LDLVFFDELMKTVPPEHGATFVSIVQSMQYLSAIAAPLIGTWLADFIGLGGALWVSAG
                     LRLLGFLLFLRKDTRR"
     gene            complement(21626..23722)
                     /locus_tag="PL000_04215"
     CDS             complement(21626..23722)
                     /locus_tag="PL000_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC88130.1"
                     /translation="MPEWIGRTIGKVRVDKYLARGGMAEVYLGTHLSLERPVAIKVLH
                     SFIEEDPELLERFQREAKAVAGLRHSNIVQVFDFDTHDGHPYIVMEYLKGPSLASYLQ
                     TLHKNKQRLSFEQIGQLFKSIIAGMDYAHSQGIVHRDIKPANIILHSRNRDFEQDTPL
                     TKSVEAIITDFGLARMAHSGQQTASGFVSGTPTYMSPEQARGSKVDHRTDIYSLGIIL
                     YELLAGRVPFEADTAITIIFKHINEAPPPIEDIYPELQSVIDKALTKFPEDRYNSGHD
                     LLVDYYKAVGLHLEVETIHATRAQTPPKPATVIQKKKSTLNPLWVGTGIFLCACVSFF
                     LLSGVVGTGLFVLPRLNSTEAPTLPPTEIHTMATEAPVVNTETPVGILRFQDGAAIAD
                     QITISANLETLPEGSQYEAWMIEDSGEQRRSIGILSAEENGTFSLTYVDEQSRNLLSL
                     YSHMEITLEPIPDTSPNPSGDVKYSTGIPGRALAHIRHLLVSTEETPNQIGMMDGMRI
                     NAVLVNQTAEAMLLAFEENKQKDLRNNAEALINLIVGKQDPAYSDWNGDGTINDPGDG
                     YGILLNGEQTGYVGGVHHHSSYSADADDSTADIRMHNEHVEISIHNIEEWAIELRDLT
                     EKILQAPADANVEAEVRKAVALANQILNGLDVNGNEAIEPINGEGGALTAYQHAYYMA
                     DMPILIGKDQMPVTGK"
     gene            23979..24422
                     /locus_tag="PL000_04220"
     CDS             23979..24422
                     /locus_tag="PL000_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88131.1"
                     /translation="MSQNAQGTQSGGQETIIQRIRTSLTQADEYTRRQQRLNSTLTIS
                     TTVGSALTAFVTALTAAQGPTVIPGVLDWQGACTIGAIFSFITTVCSGLNQQMNTSQK
                     FLLGSQCVGRLRALELVATAQSRAMDEITNEYAGILREYAEALRK"
     gene            24519..24854
                     /locus_tag="PL000_04225"
     CDS             24519..24854
                     /locus_tag="PL000_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC88132.1"
                     /translation="MEITIQEFKHCDLFKVKGRVDSASAPEFSKALEKANNDGHFKIA
                     LDMSELEYMSSAGFRALLATQRNCKRYNRGELVLVSVPERIREALELAGFTELFKTFN
                     DSIEAVGSF"
     gene            24934..25344
                     /locus_tag="PL000_04230"
     CDS             24934..25344
                     /locus_tag="PL000_04230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88133.1"
                     /translation="MPKITFPARFEYLDEIREFVGQVAREGGFNDKEVYSLQLASDEA
                     ASNIIEHAYEGVSNASFDVTCDVRGDTIIITMRDDGTPFDPTRVKQPNLKADLSERQI
                     GGLGVYLMRKLMDDVKYESNPRTGNLLIMTKRRG"
     gene            25352..28648
                     /locus_tag="PL000_04235"
     CDS             25352..28648
                     /locus_tag="PL000_04235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88134.1"
                     /translation="MRSSTRNGHPDSWDWRQLASLGEQLRNEDSLSAQRDRIASMTSD
                     LVAGVVDVWLNESVFRLPDWEDGSVFPEQPPKGGMRLAIKAGKITTQKTNRKQKKDAS
                     RKTQPPASRVTFAAVPLADHGITMGAFQVTRSKGPAFTTAELNMLQGIGQIVATSLFA
                     SHRAEVEQFRLRQLNLVREVSTQIANVLNVDELAARVTSLIQKTFNYYYVAIFTLNPN
                     SKFLRFRSSAVAPRKGKKKASIALEVEIGQGLIGESAKSGEQIVCDDVRSDARYRFID
                     SLPETQSEVVIPLKIEDRILGVLDVQSNQPHAFHPNDLLILHALADNIARAVEGARLY
                     GSLRRRADQLTLVSEVGRSVTSTLELSKLMRDAATLIHEKFKYPHVSLFTVHPNRRLI
                     VYEAGSGKRSHKLEGFTISLDDADGIMPWVARTGRTVLANDVSKHDRYLPSPLPPKNT
                     RSELCVPLIFNDDVLGLLDIQSDKLNAFTEDDQMMFEAVADTMAAAIRNADLYRSEQW
                     RRQVSDSLREVAGLVSNNVSVDEVLETILSELDRNLPIDVAAIWLLEDDDLCLSAVHG
                     CDPRLLESVCITNPDSTYAMAEALMSDAPIIRRADESLWPAGLALGYEQNYSSIAAPL
                     RVGDRSLGVLTLSHRTAGRYGHEAQAITTTFASYASVAIENARLFDAAQEQAYASAAL
                     LQVAQAVVSLNDLNEILASITRILPILVGVHRVSLYRWDSERESFITSHEFGFTEMEE
                     TTMTEKEFRLGDFPLLDFAREQNYMVAHPLDLGADPASWLQIQPEAEGEADLVNSERL
                     LMAVPLSIKHDLFGILLVEEAENGRRFRARRIEIINGIAQQAALAIQNDLLQQEMVVR
                     ERLETEVQLARQIQQTFIPHALPAHAAWQMAARWRTARQVGGDFYDVIELPNGKIGLF
                     IADVADKGMPAALFMALTRTLVRAAVIEMDSPAGVLGRVNDQLLPDTQQGMFVTAVYG
                     VLDVESGEFTYVNAGHNPPFWVNQNGNVEKLTRTAVALGVMEQPNMEERTVCLGFGDT
                     LLLYTDGLTEAFSPLGELFGDVRLMTALQAIQASTADEVLAKVEEHLNEFIESTPLGD
                     DLTMLALRRV"
     gene            28760..29224
                     /locus_tag="PL000_04240"
     CDS             28760..29224
                     /locus_tag="PL000_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNC88135.1"
                     /translation="MFDELQTRIADKNVLLLGVGNRQREDDGVGSYLVRRLQKKIRIP
                     SLDAGDVPENYIKQIENSGADFLLIVDAADFGASPGEIALLELGDLKKMGGTTHSANL
                     PLLLEIIPKNKRPETLLVAIQPGSTGPGKGLSEPVRNSLDGLDAMLVGMFKK"
     gene            complement(29248..29931)
                     /locus_tag="PL000_04245"
     CDS             complement(29248..29931)
                     /locus_tag="PL000_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88136.1"
                     /translation="MKLSVIIPVYNEVESIKVILERVQAQNLAEEIIVVDDGSKDGTR
                     DVLATLDGKNGVHIILHEKNKGKGAAVRTGMTAAQGDILLIQDADLEYDPRDYPQLLQ
                     PITEGVADVVYGSRFLGRAHRVTMFWHQVANQMLTLMTNILYNTILTDMETGYKVFRR
                     EVLNGMKIHANSFDFEPEFTAKILKRKFRIFEVPITFNPREYSQGKKIKLHDAFEAVW
                     TLIKYRFVE"
     gene            30039..31373
                     /locus_tag="PL000_04250"
     CDS             30039..31373
                     /locus_tag="PL000_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC88137.1"
                     /translation="MTRQPQQNPAPVGGFSPPQINNLEDTGLSTLWLQDLVLKVLYFR
                     GYLTGFKIAEEITLPFAGVVDQLLAALKQEKFIEVRSSQGGLGEGAYTYGITGAGIIR
                     AREAMERSQYAGPAPVPFAVYNEAIRRQKSGRLTVTTRTMRQILSQMVISENTFQRLG
                     PALNSGSSIFMYGPPGNGKTSIARAFGNLVLSQSMYIPYALYLDGQVIKVYDAVSHTV
                     APDNESGNGSGGTTSNLRSNTRRDPRWIKIRRPFIVVGGELTLEGLDLVFDDTTKFYE
                     APFQVKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRVDYLRLHTGRKVEVPFDVLI
                     VFSTNLPPKDLVDEAFLRRLRHKIEIGDPSFEEYREIFKRVAQDKRVEYNDQGLAYLL
                     QEWYIKRNRKLRASHPRDLCDQILDISSYLAVPATMSREMIDHAGKAYFVDI"
     gene            31406..32158
                     /locus_tag="PL000_04255"
     CDS             31406..32158
                     /locus_tag="PL000_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNC88138.1"
                     /translation="MFEHYPSPIILAHRGDLAHAPENTLPSFQQALQKGADGVELDAK
                     LTADGHVIVIHDMTVDRTTNGKGRVASFKLEDIRKLDAGSWFNEKFRGTQVPKLEEVF
                     ELIGKDKLINIELTNYSTPRDGLVVKVCELIKRHNIHKQIIFSSFLPSNLKIAAQTLP
                     EIPRGLLAMPGLVGLWARSFGFMFGEYQALHPHISSASREQIQRVHRLNRRVHVWTAN
                     SPEEIVRLKDWGVDGLFTDDPETAVRALGRSR"
     gene            32247..34985
                     /locus_tag="PL000_04260"
     CDS             32247..34985
                     /locus_tag="PL000_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNC88139.1"
                     /translation="MKITRILFLLTLLVTLVPVPVQAQTSTPPAAVSAILNSMTPEER
                     VGQLFLVTFTGTDTSVDSQINDLISRYHVGGVVLLAENDNFIAAPDTASGTHALIDGL
                     QQLEWQASINPSEDATAGQEDTDSAYIPLFVGIAQEGNGYPTDQILNGLTPLPSEMAI
                     GATWNTDFAMRVGAVRGQELSALGFNLYLGPSLDVLEVPAVSGGDLGIRVFGGDPYWV
                     GEMSRAYVTGLHTGSDNQLLVVAKHFPGVGGSDRLPEEEVSTVRKSLEQLKQIELYPF
                     FAVTGNAITPESTADGLLVSHIRYQGFQGNIRATTRPISFDPQALKQILALPQFSTWQ
                     TNGGLIISDDLGTQAVSEFYASGGGQFSARTAARDAFLAGNDMLYLGNITSSDFPDTF
                     TTTTRIIEFFAQKYREDPAFAQRVDESAARILAVKARLYGNFTLQNTLVPAFRLTNIG
                     TAAEVPFEVARNAATLISPELQDLAAVMPLPPQVDESMVFITDTANIKQCTDCLEQPT
                     LAVDALQQAVLQLYGPQSGNQTNNFRLASYSLQNLETLLNGEETPFIEDDLTRAGWVI
                     FSLTDAAQGQANLISRFLRERPDLLREKRVILFAFGAPYYFDTTTISKFTAYYALYSK
                     QPQFVDVAARLLFQELSPVGASPVSISAVGYELISVMAPNPNQIIPLFLDLKPVATGT
                     ESPLTPEPTPIPLFKIGDTIAIRTGVIQDRNGHPVPDGTVVQFSMKLTGEGAGILQQE
                     DAVTTQGVARAAFGLDKPGLLEISVVSEPAVISEVVQLDVSQSGAVAVTVVVPELTEA
                     IEPTPEATPVEVVDAYISKEGYPRFSAWLVAMLFMVFSIWVVYSTGIRIIDRHSALRW
                     SLGIALGGLLAYNYLAFGLFNSMSWLTVSGLSGVVLFVFIGELMGFVAGWVWSRRR"
     gene            35129..36139
                     /locus_tag="PL000_04265"
     CDS             35129..36139
                     /locus_tag="PL000_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88140.1"
                     /translation="MAAQSTKNKEKQPPILFDKTQAIIKQVNKMLGGTLVTYFNNPRG
                     SVCHDDVLALHELLEKIGHQPKIFLFLKSSGGNGQASLRLVNLLRQYCDEVIAVIPLE
                     CASAATMITLGANEIQMGPMAYLTPVDTSLTHSLSPIDRDNDRVSVSLDELTRVVRLW
                     QGQNADARENPYKELFQHVHPLVIGAVDRAESLSIMLCKELLAYHIKDESEAERIASS
                     LNAKYPSHGYPILFEEAKRIGLKVKRLDPEINILLLELNELYSEMGQRATTDFDDTHA
                     HGNEILNIWESTSVLVYYQQDKDWFYRTEERRWISLNDVSRWRRMIQVGNKVEKSILH
                     IS"
     gene            36253..36852
                     /locus_tag="PL000_04270"
     CDS             36253..36852
                     /locus_tag="PL000_04270"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HNC88141.1"
                     /translation="MANQPQLKFNDPAPDATLLDANGKPIQLSSLWKDRVVVLAFTRH
                     FGCPQCKEMVDQLIDAHQALTEKGMLLAIISHASAESARQFCEQRAPSATCLADPDRS
                     AYHAYGLYRGTFWQTLLSPRIWLSNRKLARTKGYKPELPSPGQDAYQMSGTFIIGPDG
                     RIRLPYYYEDIADHLPVDLILHGIMGVDWKRPYDSKPVV"
     gene            36858..38018
                     /locus_tag="PL000_04275"
     CDS             36858..38018
                     /locus_tag="PL000_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88142.1"
                     /translation="MTSFFLATYLAIKEVFRNRGRFLLVALVIALITVLVLFIAALGE
                     GLSDANRQYVANLDAQLIVFLEKSDYSITASRLETNTAKAVRRVDGVADAGPIYTSNS
                     EIVSLPEPLKISMLGVEAGRPGMPSLLEGRYFRGGAAREAVMDMSVAQRTNLKIGDEI
                     EIRSTQGTEDQFYTLKIVGLVDKQAYFFQPTIFVPASTWELMRPQSESDLNSDTPFPN
                     IVAVKLTDPTQVEEMKARLEDQVSNIEVADIETTINNIPGYTAQQGTVQTQGAFTLLI
                     GILVIGGFFQIQILQKVPQIGVLKAIGSSNFVVGLSAVIQIIIVTAMGVGIGAGLNYL
                     FSLGLPPNIPLAFNGTRSLVAIVLLLFIGPLGGMVSIIYAVRIEPLKALRLG"
     gene            38026..38757
                     /locus_tag="PL000_04280"
     CDS             38026..38757
                     /locus_tag="PL000_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88143.1"
                     /translation="MSNIALEVRDLVKSFTLDGATINAVDHVSFQVRSGEFVALVGPS
                     GSGKTTLLSILAALLTPTSGQVLIDGQDLAQMNEKKRVKLRREKIGFTFQSNNLIPFL
                     TAQENVEFMLRLNGSLGRAGKVRSGELLARLGLAERLHNLPAQMSGGQQQRVAIARAL
                     IHQPAVVLADEPTASLDTERAFQVVETFAHLIHENERAGIMVTHDLRMCQYVDRVLQM
                     QDGKLVREYSTKEEIMELALGTVKH"
     gene            complement(38829..40823)
                     /locus_tag="PL000_04285"
     CDS             complement(38829..40823)
                     /locus_tag="PL000_04285"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HNC88144.1"
                     /translation="MKIHIKFSLALIVSLLVISNISPSHSITGIARAQANFATVTYDP
                     VNTIITNPERGFLHTNSPCESDIFDLATLQNYRSAEHISLVLCNFYLTSFKSSAISQA
                     TLNTLQAQLNTIRSAGLKVVLRFSYTSTDNVDASLSQILSHIDQLAPILQSNSDVIYV
                     WQAGFIGQWGEWYYTNHFGDQGVISAQQWADRKAVVEKMLQVLPSTRMVSLRAPFYKR
                     TMYQAAAITPDQAFNGSSISRIGQFNDAFVSSPDDMGTYTNIPEEYPWLEAETAYLPM
                     GGENNTWAPPRTNCTTALEEMDRFHWSYINIDYLGTTISNWNSQGCLDTMKKKLGYRF
                     TLIEGTYPDTAQTGSGMDVHISIRNDGFAAPINPRPGELILRNSSSGEIYHLPLKSDP
                     RFWTAGATTTIQETIIVPTNIPAGSYELLLNLPDAEDSLSSRPEYSIQLANANTWESA
                     TGFNNLLHTISISAGIPTTFADVPFDHPLHDYIEALYQAGYTAGCSTDPLMYCPDTIL
                     DRAQSAVFMLRGQMGSTYSPPPAPWDTFADDWTGFEWAEPWAEGMWQEGLTAGCQPSP
                     LMYCPATQLPRVEASVFGLRMKYGVNYTPPAGTGTLFADMTDTSYWGIGWAEQAYQDG
                     LLPACGTDSGTGKPLFCPSELLDRAWGAYLIVKAKNIPLP"
     gene            complement(40963..42303)
                     /locus_tag="PL000_04290"
     CDS             complement(40963..42303)
                     /locus_tag="PL000_04290"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNC88145.1"
                     /translation="MDNKDLLKRVKEDNVKFLSLQFTDVMGAVKSVDMPIRHLEDVLK
                     DGAWFDGSSVEGFARIQESDMRLKIDPETYAVLPWSAADSKRARVFCDIYTPGGDPFD
                     GDPRGALKRILKKIADRGWKLNIGPEPEFFLFKASDGSGVHPVPHDTGGYFDFSSFDE
                     AVVVRTALMEALDAMGLDVEVGHHEVALGQHEIDFRYADALKAADNVLTLKYTVKAIA
                     AQHGLTASFMPKPVYGINGSGMHCHQSLFDIKTGDNLFFDAKDEAHLSPLAYSFIAGQ
                     LEHARALAGVVAPTVNSYKRLVPGYEAPVYIGWAQQNRSALIRIPRYTEGRDKAVRAE
                     LRFPDPSSNPYLAFTVMLAAALDGIDKNMKAPKALNNINLYHLDKAERTKLGVTELPG
                     SLAESLKELEKNEVLKSALGEYLFEAYLRAKWEEWDEFRLRVTDYEITKYLETA"
     gene            complement(42365..43294)
                     /locus_tag="PL000_04295"
     CDS             complement(42365..43294)
                     /locus_tag="PL000_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC88146.1"
                     /translation="MKSKIWLPLLALYIVWGSTYLGIKVSIETIPPFFHAAVRFLISG
                     MILVVWQRSAGHQMPTRKQWVSAGIIGTFLLLGGNGLVAWAEQFIPSGIAALIIGSMP
                     MFLVIGEAIRPNGVKPTWQGIVGLLVGFAGIFILVGPSEISGSTEKLNPLGVIALLSA
                     CLFWATGSLYSKTADLPKSSLMNTGAQMLVGSVSLLIVSLISGELNGWDVSAVSTRSL
                     VGLAYLIFIGSLVGFVSYGWLLQNAPISLVSTYAYVNPIVAVLLGVWFANEALEPRIW
                     LATAIIIGSVIFINSTKPKVKHEAKVMTAEQGH"
     gene            complement(43335..44201)
                     /locus_tag="PL000_04300"
     CDS             complement(43335..44201)
                     /locus_tag="PL000_04300"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC88147.1"
                     /translation="MESTTELSQHYQLIEQAIQYIEINVHRQPELDEIASAIGLSEYH
                     FQRLFTRWAGISPKRFMQFLTKENAKDLLTRSENLLETTHQVGLSSLGRLHDLFVNTE
                     AVTPGEFKSHGAGLIIRYGLHLTPFGKCLIAATERGICHLGFVESSEGNAIDNLVADW
                     NQAKMIEDYKTTAPLIDRIFSTPTPDTPLNLHLRGTNFQIKVWEALLKIPAGALTTYE
                     QIAAQIGNPNALRAVGTAVGHNPIAVLIPCHRVIRKSGEFGNYRYGPARKKALLAKEF
                     AQSRLTSDFQGM"
     gene            complement(44454..44631)
                     /gene="ssrS"
                     /locus_tag="PL000_04305"
     ncRNA           complement(44454..44631)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PL000_04305"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            44770..45780
                     /locus_tag="PL000_04310"
     CDS             44770..45780
                     /locus_tag="PL000_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNC88148.1"
                     /translation="MATVPGIDVSYWDAGIDWPKVRATGQRFMFAKATEGISYKDLTF
                     DDNWFGAKSAGLLRGAYHFFRCNVDAKKQADYFIDYVRTVKDDGELPPVLDLETNDGM
                     TKDKIVPAVKVWLDRVESAFGKKPIIYSGQYFLQDYVVIAGGGPPTWAKEYPLWLAQY
                     PNQYQEGMKPFLPRGWFSWTFWQYSDKGVVNGINAKVDMNIYNGTLEELYKFAGAKIP
                     DQTPKTHTVKKGDSFESIANSYGVTVRELVMANPQLLVTGSKLTVPVAIAIPQEGDSS
                     STDTGSGDTGTVSTRTYTVKAGDTLSVIAVKYGTTVAMIASANNITNINNIKVGQVLV
                     IP"
     gene            complement(45867..47210)
                     /gene="asnS"
                     /locus_tag="PL000_04315"
     CDS             complement(45867..47210)
                     /gene="asnS"
                     /locus_tag="PL000_04315"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNC88149.1"
                     /translation="MTEHVSVSKIANYVGQDIILKGWVYNRTDKGKLAFLLVRDGSGF
                     VQCVAFKGDLTPELFDQIAHLPQESSVIITGSVREDKRAPGIPGGFEVGIKDLQVVQA
                     ANLDYPMSLKDHGVDFALDNRHLWIRMQSQWAILRVRVRVMSAVREYLDGQGFFNLDT
                     PILTPAAAEGTTTLFETEYFDEGSAFLAQTGQLYNEANIFAFGKVYCYGPTFRAEKSK
                     TRRHLTEFWMLEPEIAFCDLDQLMDIEEGLITHIAQSVLKDCSAELKFLGRDLTKLQN
                     IVGPFPRMSYDDAAKYLIDLYEKETDPERKELLKFEWGMDFGAPHETELTKLYDKPVF
                     VYGYPSAVKAFYMEPWPDRPEVCKSVDLLAPEGYGEICGGSERMSDPDKLLARIQKEG
                     LPEEAFKWYLELRKYGSVPHSGFGMGTERVTAWMCGIEHIREAIPFPRTIKRIYP"
BASE COUNT        11041 a        11714 c        12462 g        12006 t
ORIGIN      
        1 accacttctc ccaatgtacg atctcatcca gcgatacccg tccgcctttt ttgggggcgg
       61 cggagatttt ctcttcgtcg agggcatagc cgaaggagag ggcgatacgt aaatgccatt
      121 cgggagggaa tgtcaaaatt tggcgagtcc gttcaaagtc atagagagaa gcggggcatg
      181 atccaatgcc caattcccat gcggcgagct gcataaaggc ggcggcttgc cctgcgtcaa
      241 acatcgtctg gaatttttcg gtgggttcgg gggtgagaat ggccacgcac agggccgcgc
      301 ctgcaatatg gcccgcccac tctccgcatt gtgacaaagc ctttagcgtt tccttttcac
      361 gaacggcaat gaaacgccag gcctgattgt tcttcgaaga ttgcgacctg cgccctgcat
      421 tgagaatggc gtggaccaca tcatctggca ggggtttgtc ttgaaatttt ctcacggcgc
      481 gttttagacg gatggcttca gatacgttca tggttcctcg cttttcgagg attatagcct
      541 accgttgaaa atggatgttg ctcttggcac tatcaaaaag ggaagatagt caataggcag
      601 tttgacctca ttttgtgata atgaaactgg taatttgtca atttttatat aaaaacagga
      661 gccccctatg accttaccca ctgtttcaga aaccacccta ttgaaagaat ttgcctacac
      721 cctgcgcccc gtagagcgtg gatatgcaaa ccgtacgctg tacatcaacc tgaacgacaa
      781 taccatcctg gaaaagcccg tcacccaaca aatgaaagac ctgttcaccg gcgggcgcgg
      841 atttgcactc aaattactgt gggatgccgt aacccccgaa acgaaatggg atgacccgca
      901 aaatgaactg gtcattgcca acggcccgat ctgtggaatc acagcctacc ccggcagcgg
      961 caaatccaca gttgttaccc tgagcccgtt gaccgaaaat gtgatcgaca gcaacgtggg
     1021 cggatatttt gcccccttcc tgaaattctc tggttttgac gcgctcgaga ttcaaggcaa
     1081 ggcagccaaa gatgtgatca ttttcattga cggcgatact ggacgagtga ccatcgaagc
     1141 agactcactc gaagctgtcg actcacacca gatcggcaat gtcctcaccg aaagatttgg
     1201 cggtgacgaa aaaggcaaac gcgccatatc cgtgctttcg gcaggtcagg cggcggaaca
     1261 tacccgcatc gccatgatca attccacctg gtacgatgtg cgccgcaagg aagcccgttt
     1321 caaacaggca ggccgcggcg gtccggggcg tgtcttccgc gataagaaga tcaaagccat
     1381 cgtggtacgc tactccgata tgggcggaga caagaacggt gtggccgaca tgccgctcat
     1441 ccgcaaggct ggtcagcgta tcaacaagga aatcgccgac ctcgatgaca agcagaatca
     1501 aatgcgaaaa gtgggcacgg ccaacatcgt cgaagtaatg gaccatttcg atctgctgcc
     1561 cacacataat ttccgctatg gatcacatcc cgatacgcat aaaatcgatt cgaaagtttg
     1621 gaaggaaaaa ttcacacagg gattacccga cggctgctgg ctgggctgca ccatgtcatg
     1681 ttctcatggt gtggatcact tcccgcttca aactggacct tacgcaggcg actgcgttct
     1741 ggtggatggc cctgaatatg agaatgccgc gggactgggt tcgaacatcg gcaatttcga
     1801 cccgcaaaaa gttttggaac tcaatttcta ttgcgatacc tacggcatcg acagcatctc
     1861 atttgcaaac tgtgttgcct ttgcgatgga atgttatgaa gaaggcatta tcacaaagga
     1921 acaaacaggc ggtttggaat tgaagttcgg caacggctcg gctgctttgg atctcattca
     1981 ccaaatggca cgcggcgaag gctttggtgt gatcgtgggg cagggtgttc gctatatgaa
     2041 agaactattt atccgtcaat acggcgctga cccgaaattc atccatgata ttggcatgga
     2101 gatcaagggc atggaaattt ccgaatacct caccaaagaa tcgctggctc agcagggtgg
     2161 ctacggcatg gccaccaaag gtccacagca tgatgaagcc tggctgatct tcatggatca
     2221 agtgcaaaac ctgctgccct ctttcaaaga caaagccgaa gctttgcatt acttccccat
     2281 gtggcgcaca tggttcagcc tgcacgggtt gtgcaaactg ccgtggaacg acatctcacc
     2341 cgaaaacaac aagcagacca aagaacccgc caaagtggaa gaacacgttg aaaactatac
     2401 ctggatccat cagggcgtca ctggtaagcc aacggttgcc gaagacctgc tgcatcaatc
     2461 ggagagagtg tacaacttcc aaaaggtctt tgccttgcgc atgggtcgtg taggacgcaa
     2521 acacgattac ccgccttacc gtgcaatggg acctgtcaca gctgctgaat acgaatcccg
     2581 tcaggatcgt tatgacgaac aactcaaacg gctggttgaa gttgaccccg caaacctgac
     2641 cacacaggaa aagatggcgc acctccgcaa ataccgcgaa gaacaatatg aacaattgat
     2701 ggacgcggtc tatcaaagaa ggggctggga cgcgaacagc attcccaccc tcgaaaaact
     2761 tcaccaactc ggcattgatt tgcccgaagt cgtagaggtc gtcaaacagg cgcagaaaaa
     2821 gtaaatcacg aaatcactgt tttacagaac cagaaaattt aacgccaggg cgcggagatg
     2881 caaagccatt gcaatccccg cgccctggcg gctttgtgtt aaaaattcga gcggatggtt
     2941 ttgtatcatc gcaaatggcc ttttccctct gcggttataa ttcgtgcaat ggattcgcaa
     3001 atcacatttc gacaatatct tttttcaacg cttgccctcc tgctcatcgg ctggggcgga
     3061 cttgccctgc tgttgattta ttctgtgccc ttcgtatggg cccgttgggg attctacgtc
     3121 tgcggcatta tggcgcttac aggcacggcc ttgcctgttg tctattttct acaccgccgc
     3181 tttccgacca ctccacaagc cgagccaggc gttattgtgc ggcaggccat gtgggtgggg
     3241 gtgtatgggg ccactttggc atggcttcaa cttgggcgtc tcgtcacact ttatgtaatt
     3301 ctcggtttgg cgggcggcct gatcgcaatt gaatatttca ttcgcctgcg tgaaaaagcc
     3361 aaccgccgcc tgcccgatat ccaatgacaa cgcttcgcga tctaccttcc attgatcaaa
     3421 ttctccaaac acatttggct gcgcagctca ttgcccgcta cggcaggcca ttgaccttaa
     3481 gcgccattcg tttcacattg gatgaaataa gagcccgctt caaatctgga cagataactg
     3541 ccctgcctct acgagatttg atcctcgctc aggcagatgc caccctcacc gcatggacaa
     3601 agccgaccct catcccggtc atcaacgctt cgggggtgat tctgcatacg aacctcggcc
     3661 gtgccccgct cagcgatgac accatccgcg ccatggatgt ggcttcacgc ggctattcca
     3721 acctcgaata cgatcttgaa aaaggcgcgc gcggttcacg gctcatccat gctgaatctg
     3781 ttttgcagag actcactggg gctgaagcgg cgatggtggt caacaacaac gcttcggcgg
     3841 ttttgctggt tctttctgca ttggcgaata aaaagcgggt catcatttca cgttcgcaat
     3901 tggtggagat cggcggcggc ttccgcattc ctgatgtgat gaagcaatca ggtgcaaggc
     3961 tggtggaagt gggcacgacc aacaaagtcc atttgcggga ttatgaagaa gccttgctgg
     4021 gtgcgaatgc cattgtcatg cgggcacacc catcgaattt caagatcatt ggttttaccg
     4081 aagagcccga attgaaggat gttgttgcgc tggcacatgc cgccgacacg attctggtgg
     4141 atgatctcgg ttctggcaca ttcgttgaca ctgccaggtt tggcctcagc catgaaccca
     4201 ccgtacaaga atcccttgcg gctggggcag atattgtctg tttttctggt gataaactgc
     4261 tcggcgggcc acaggcaggg atcatcatcg ggaagcggga tttgatcgag aagattcgca
     4321 agcatccgct ggcgcgtgct gtccgcgcgg ataagacctg ccttgcgggt atcaccgcca
     4381 cccttttgca ttacctcaaa gatgaagcgg agcgtgaaat tcccatctgg aagatgatct
     4441 ctcttacgcc gaagcaggtc aaaggtcggg cggaagcgtg ggctgccaat ttggggcagg
     4501 gaacggtcgt ggagggtgaa tccacagtag gcggcggaag cctgcccacg gaatccttcc
     4561 caacattttt attgtcatta tcggtctcat ctgccgataa attcctgaaa aagttgcgcg
     4621 aacaaaaccc gccgatcatt gcacgcacgg cagataataa agttttgctt gacccgcgca
     4681 ccgttttgcc cgaacaggaa ggggccttgt tggttggttt gaagaatgtg atgaaaaann
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt ttgcccgaac aggaaggggc
     4861 cttgttggtt ggtttgaaga atgtgatgaa aaaccttaac gcgaatgctg cgaataagcg
     4921 aatgtaacga atggagtttg ggatggacga aatttatata gtagagaaag aactgtctta
     4981 tttgattatg caagtggctt ttgaggtaca taatacgctc gggccaggtt ttccagaagt
     5041 aatttatgaa gaggctatga atcgtgaact cgcaagacac ggagttaatt tggaacgtca
     5101 aaaacatatc aaagtttttt ataaaaacga accaattggt gagtttgtcc ttgataatgt
     5161 agctaatgga cgtgtaattc ttgaatacaa ggctgttgcg gagattgcga aaattcatgt
     5221 gcaacaggcg ctttcatact tgaaagcaac cggtcttgaa ttggcgattg tcattaattt
     5281 tggatcgaca cgggttcaat ccagtcgtgt tgttttcaaa aatggtaaat ctaatttccc
     5341 atcgcagacg ccacggtcgc gtaatgttct gcgcaacgat taaaaattcg ttttattcgc
     5401 acattcgctc tattcgcgtt aaagactctt ataaaaagga accttatgaa atcagaactt
     5461 gataccctta tgcaagccaa agacatggat gccattctca tctttggcaa tgccgaacac
     5521 aacccgccca tgtattacct gacaggtggt ggacatgtca gccatgccac tctgatcaaa
     5581 aaacgcggcg gcgatgcgac ccttttcttc ggtgatatgg aacgtgacga agctgcaaaa
     5641 agcggattga aattgattcc ctacagtact tatgattttg aagagatcta caaaaaggcg
     5701 aatcgaaacc ccacgctggc gggcgccatg cgccttgaac tgatgctcaa agatgtgggt
     5761 gtggaatcgg gtcgggtggg ggtgtacgga acatatgaca tcggtcccgt ttttggatta
     5821 ttgatgcagt tgcaaaagct gatgccgaat ctcgaattta tcggcgaacc gcgggaagat
     5881 tccattttcc tgcgcgcaat ggaaaagaaa gatgaagccg aagtggagcg tatccgcaaa
     5941 atgggcaagg tcaccacaac cgtggttggt cgtgttgctg attttctgac gtcctgcgaa
     6001 gttcgtgaag atgaggttct tttgaacgaa aatggatccg ctctgaccgt gggcgatgtc
     6061 cattcgaaga ttcgtttatg ggtatctgaa ctcggcgcag aattaccttc tggattcatc
     6121 tttgccattg gccgcgatgc aggcgtgccg cattccgcag gcaacccagc cgatttgatg
     6181 aaacttgggc agacgatcgt gttcgatatt tatcctgctg aggcgggtgg cggctactac
     6241 tacgatttca cccgcacctg gagtttgggt tacgccacgc cagaggcaca ggaattgttc
     6301 gatcaggtta aagagatcta cgaccagttg aacgagaact ttgacctgaa tgtcccgttt
     6361 aaagattacc aaaaaatgac ctgtgaatat ttcgggggca agggacataa aacccccatg
     6421 aactccaaat cacctgtcga tgggtatgtt cacagcctgg gtcacggcgt ggggctcaac
     6481 attcacgaac gtccattcag cggcatcaat accagcgatg aagagcggct tgcgcccggg
     6541 gtggtgatca cctctgagcc aggcttgtat tatcctgaaa agggtatggg cttccgaatc
     6601 gaagatacaa tatgggtgcg ccccgatggg cgcatggaga tccttgcaga ttatccgtat
     6661 gattttgttt tgccgatgaa aaagtggaag aaaaaataat ggacctgaaa cccgcccgaa
     6721 tccttgccct tgaaacctcc tgcgatgaaa ccgcctgtgc catcgttgaa aatggccgcg
     6781 cattgctttc ttctgtggtc gcctcacaga tggagattca tgcgcgctat ggcggggtgt
     6841 atcccgaggt ggcttcacgc cagcatgttt tgagcgtaac gcctgttgta gagcaggccc
     6901 ttgcacaggc tcacctcacg ctcaaagata ttgacgcgct ggccgtcaca caagggcctg
     6961 gccttgcggg ttcgttggtt gtggggatga acatggcaaa gggacttgcc ttgggatccg
     7021 ggcttccatt gatcggagtg aaccatcttg aagggcattt gtattcggcc tgggtgcatg
     7081 attcgggaga actcgtaccg cccgcaccac aattcccctt gatggctttg ctggtttcag
     7141 gcgggcatac ggaactgaat ctcatgaccg atcatttgac ctatcaaagg ctcggttcaa
     7201 ccctcgacga tgcagctggt gaagccttcg acaaagtttc ccgcctgttg gaacttggct
     7261 accccggcgg ccctgccatc caaaaatcag cggaagaggg cgacccgaat cgtttccatt
     7321 ttcctcgggc atggctcgaa gggacatggg atttttcgtt cagcgggttg aagaccgccg
     7381 tattgtacga gatccgcgaa ttgcaaaagc ggacacacac cctgcccgta cccgatctgg
     7441 ctgcatcttt tcaaaaagct gtggtggatg ttcttttcaa gaaaaccatc aatgccgccc
     7501 gtgaatttgg cgcgaaggaa attttggtgg ctggtggcgt ttcagccaat cgtatgctgc
     7561 gccagacctt tagggcccaa acggaatttc ctgttcatat cccgccgctg tcgttgtgta
     7621 ccgacaatgc cgccatgatc gcctcggcag ggtaccatcg ctatgcactt ggtcaccttt
     7681 cgcagatgga catggatgtt ctgccgacct ggccgctgcc gtaacgataa aatcatgcat
     7741 taaaaccaga aggccgatga cttgcccaag tcatcggcct tctggtttag ggagaatgta
     7801 actgtatttt agaatcgtca cctaaactga acctgagcat tgattaagag ttggatgaga
     7861 gaaaatgagc ctgaaattta aaacatcgag gccccgtttc aaacttgccc aagtctgaaa
     7921 aatcggggcc cgcgataagg agaatgtaac tttattatat tcagtgagct aaaaacagcc
     7981 tgaatgggaa atgaacacag gattagagat tcggtcaacg gagtgcgttg agaagttcgt
     8041 ccaaagtaat gggaccttcc cgtagaatga cgggtgtttc acctgtgcaa tccaccagtg
     8101 tggatgggat tccgcctggg gtttggccgc catttaaaat gagcggaatg cggtcgtgga
     8161 gttgtgtgta aacttcttgt gctgtggttg ggctgggatg tcctgacata ttggcagagg
     8221 taactgccat ggggcctgcg gcagttaaca aggtgagggc atctgggtgg tttgggattc
     8281 tgacggctac ggtggaggtg gcagatactg ccggggggag agtctgcttt ttggggagca
     8341 cacaggtgag gggtccgggc cagaagcggg ccatgagggt gtaggccatg tctggaatgt
     8401 ggtcagtgac aaggtggagc atatccatgt tcccgatcaa aattggaatg gctttttcga
     8461 tgggacggtt cttggcgatg tagatgtttt cgatggcggc gttatcaaag gcgagcgcgc
     8521 caaggccgta gactgtatcg gtggggaagg ctacaaggcc gccttgttga agggttaaca
     8581 gggcatcttc ggtggaatga gatttactgg ttgagatgat ctttgttgtc attcaggttc
     8641 ttcatttcgt gcaaaaagat ttccacgata tttgggtcga agtgtgcgcc cgactcattt
     8701 ttgatgtatt cgagtacttt ttcaggcggc catgctttgc ggtaggggcg gtctgaggtg
     8761 agcgcatcgt atacatcggc aacggcaaag atgcgggcag agaggggaat ctccagcccc
     8821 tttaatccgc gcggatatcc gctgccatcc cagcgttcgt gatggctgta gggaatatcc
     8881 atggcggggc gcaggtattc gatggggtat aacaggtcgt aggcgaattg cgggtgttga
     8941 cgcatgacct tcaattcaag gtctgtcagg ggaccatttt tgtgcagtat ctcatcgggg
     9001 acgccaagtt ttccaatatc gtgcagcagc gatccgcggc ggatgtggag cagttcctct
     9061 tccgtaagcc tcattgcctt ggcaattcta taggtaagtt tggtgacgcg ctgcgtgtgg
     9121 ccttcggttt ccttgtctcg caggtccagg gcgcgcgacc agctatcaag tgtggtttca
     9181 taggaagagc gcagatcggt gatgaggaat tgatttttat tcagggtgtg attaagctgc
     9241 caggcagtga agaaggtgaa aaatgcgatg gcaaaaaggg cgagtacccc ggtgaaatta
     9301 tagctaatca gggagtcgta gtaatagctg gagtaggtgt acccaagggt ggtgagcagg
     9361 gcataaaaaa aggatgcaat cgggtggagg acaaaactgg aagtaatgat ggggagagcc
     9421 agaatgacca tcgtaaattc aaggtcatga ggttcgtaca ggagcaccgc cccaaaaatg
     9481 gaaatattaa tgcaggcatg ggcggccagt tttgtgtagc ctctgcggtt gagatgccaa
     9541 aaaaggaata aaaccgacag aacgatcaca tcttgaataa tgtagttgaa gtacattccc
     9601 tctttagcta tgatcaaata ggcaagattg gagatcgtga catgtaccag gatgaccacc
     9661 atcatgatca ggtacattgc catcagacgg cctcgtcgct gttcgctggg ttccactgcg
     9721 tctgtatctg tgacctgata gtataggcta tttaatttgg ggactattcc gttgtagcga
     9781 ttcatagact gtctgttact caattggaga agtattacat ggtttttcag gttgaaatct
     9841 tggtgtttgc ttgcagtttc gcgcctggac aattttgcac ggatttttgt cgatgcgtgg
     9901 tgctgagtat ggcatgattt tggaagggcg gcaacctgcg cttaatttgc atcgggagat
     9961 gggatgatct ccagcaggcg gtctttgcct gccagatcct gatgcagatg aataaccgca
    10021 ttggagaagg aatcgaaggc aagaccggcc gcctgcaccc ccaatgtgga ttcgatctca
    10081 aaaagcatca tgcctcctgg tttgagccag gcaggcgcag ccttgaaaag gcggcggtac
    10141 acatcaagcc catcagggcc gccatcaagg gcaagggtgg gttcacggcc aaagatggga
    10201 agctgccgca atgtgctggt ggggatgtag ggcaggttgg cgcagagaag gttgaaagat
    10261 gaagagtgaa ggttgggtga tgaagcaggg agcaggtcgc attccaggaa ctgaatttga
    10321 ttttgaacgt ggaatttttc ggcattgcct tttgccacat tcaaagcggc gggcgaaaga
    10381 tcagtggcaa ggatatgggc atccgggatg ttcgcggcga tggatacggc aattgcccct
    10441 gaaccagtgc cgatatcagc cacgcttcgt gcttcggggt gcctgttcag ccatgcgaga
    10501 gctttctcta ccagaagttc tgtctcaggg cgtggaatga ggacatcatg ggtgatgtca
    10561 aaatcaagac cgaagaattc ccaatgaccg attatgtagg ggaggggttc acccgctatc
    10621 aggcgggcaa atgcttgttc tgcttcgaca agctggggcg gggtaagagg cgtttcaagg
    10681 tgggcaagca gccatgttcg cggacgttta ataacatgcg caaggagaat ctgcgcgtca
    10741 agcccatcca gagcagcctt gtgattgttg agatactctc ccgcattcat gagttgaacc
    10801 caaagaaaat gtggcgacca tgcagccgcc acattcgatt attcgtcatc atccacaccc
    10861 gtggcggcga ggcgttcgga ctcatcccgg gttgacaact catcgatgaa ttggtcaatg
    10921 gccccatcca tcactgcaga aagattgtag ctagaaaagc caacgcgatg atccgtaaca
    10981 cggctttgcg ggtagttgta agtgcggatc ttttccgaac ggtcgcctga acccacctgt
    11041 gaacggcggt cggcttcaag ttcggcacgg cgtttttgtt cttcgatctc gtacaggcgt
    11101 gcccgtaaaa tgctgagggc gcggagtttg ttttgcagtt gcgagcgttc gtcctgacag
    11161 gtgacgatca tgcctgtggg tttgtgatac aaacgcaccg cagtggagtt cttttgcacg
    11221 ttttgtccgc ctgcgcccga agagcgatag acttcgatct cgatatcgct ttcgggaatg
    11281 tccacttcca catcatctac ttcggccagt acagtgacgg tagccgtgga ggtgtggatg
    11341 cgtccctgcg actctgtggc tggcacgcgc tgcacacgat gcacccccga ctcatatttc
    11401 agttttgagt acgcgccctt gcccttgact tcgaagatta cttccttgta cccgccgacg
    11461 ccgatggcac tctcagacat gagttcaacc ttccagcgtt tgccatccac atagcgggtg
    11521 tacatgcgga acaggtcagc ggcaaaaatg gcggcctcgt caccgcccgc acctgcgcgg
    11581 atttcaaaaa tgacattgcg ttcatcgcgc gggtcttttg gcaccagcat gcctttgatc
    11641 tctttttcga gcgcttcgat ctttggctcc agatcatcca catcggcctt ggcaagcgcg
    11701 atcaattccg aatcgttttc agaaatgatg atggacttgg cctcttccag gcttttcagg
    11761 gcctggcgat attcgcgaat cttgttgtag agcggttcca attcaacacg ctctttgttg
    11821 atctccccag cgcgctggta gtcgctgcct acttcgagca gttcacttcc cagttgttcg
    11881 tatcgttctt caatgacttg tagtttatcg agcatgattt gattccagtt ttttgtatat
    11941 taaacctctt gggtatcgag gtttggaatg ttttatattc tcttccaatg tgcgaaataa
    12001 aaggcggcac acaaagctgt gccgcacagg cgaaacgaaa acaaccgtga acgtacctag
    12061 taatttgcga ctgccgaaag caccatcgaa aggatcagaa tgaccgcaaa caccgcgaac
    12121 aggatctgtg cggctctctg gtttgaattc ccttggtcag cgggtttccc ttgttttttt
    12181 gttttggatg tttttccagc catctttcca ctcacttcgt aatatgtctg taaaaggctt
    12241 gcgccattac tctttcaagt tcgtcaattg taacaggttt catcacgtag ccgtctgcgc
    12301 ccatttcaag ggctttatcc accatcacat cagccgcctc agaggaaagc atgatgacag
    12361 gcaggtcttt ccactccttg cggcggcgca gaaactcgag catatccaac ccgctcactt
    12421 caggcatgtt gatatcgagg acgaacaaat caggccgccc gccagccagc aaagcctggg
    12481 cggcagggcg gggattaatg aaatgtttgg tttcgcaatc cagcagtttg agcatcaaac
    12541 tgattgcgtg tcccatttcc tcgtcatcgt ctacgatcca tacttgtttc attccagatg
    12601 tgccttaatg ctttctgccg ctgtttcgtt catgcctttc acagcagcca agtcggtaac
    12661 actcgcttcc ttaatcttat ccagagaacc gaaatgtttc aagagtgctt ttctacgggc
    12721 agggccaatg ccggggatgg aatccaactg tgaggccagt ccctgcttcg atcttcgtgc
    12781 gcgatgggcc gtaatgccat aacgatgcgc ttcatcacgg atgcgctgca caaggtacag
    12841 tccctgcgaa tgacgcggca acagcaacgg cttgctgtta tgcgggaaga agatttcctc
    12901 ctcctgtttg gccagcccca ccacgggcac cttatcgtat agttcaaact cttccagaac
    12961 tctcaccaca cggcccaact gccccttgcc gccatcgatg atgatcagat caggcaggaa
    13021 ggagaacgac gcgtcttttt tcgaccccgg gcctgcttct gtttcctggc tggatagcca
    13081 ccgtttgaat ctacgggtta gcatttcctc catcgaagca aaatcatcag gcgcgccgat
    13141 gctggtgctg tcaatgttga atttgcgata cagattcttg gcaggcacac cctgctcaaa
    13201 aacgatcatc gcgccgacgg ttgccacacc ctgtgtatgg ctgacatcgt aacactcaat
    13261 tcggtttggc ggcgcagaga gtttcaatgc gttctgcaat tcgctgagcg cctgttcctg
    13321 tttgtgcgca tcggcctgcc attgggcacg caatgcctgc agggtttcag aggcattttc
    13381 agcggccatt tgcaccaact catgcggttg accttccttc ggcacgaaca attccacttt
    13441 gctgccgccg cgcttggatc tcagccattg gccgatgatc tttgcctcct ccacttgaat
    13501 ttcttcaggg atcatcacct gatcggggat ggtggcggct tcggtgtaaa actgcttgac
    13561 gaattccgcc attacctcgg tatcagcggt atcttcagtg ccttcgagga tgaaatactc
    13621 gcggccgatc agctttccgc cgcggatgaa gaagatctgc acacaggctt cgccgtctga
    13681 acgagccatt gccagcacat ccgaatcttt gtaatcggtg gcaaagatga tcttctgccg
    13741 ttcgatgatg gaactgatgg cttttagctg gtcgcgcacg gcagcggcct tttcgaaacg
    13801 catttcttca gacgccttga gcatttcggc ttcgagacgc ttgatgatct cttcactgtg
    13861 cccgctgagg aaatccatca ggtcggagat catttggcgg tatcctgcct ggtccacggc
    13921 tccgatgcag ggggcaaggc acagtttgat gtcaaagtaa agacacgcac gtttgtcgag
    13981 gcctgtaatt tcacggtcgc atgtcaaata cggaaagatc ttccgcaata catccagcgt
    14041 ttgatacact gcccatgccg aggtgtaggg accaaaataa cgtgaaccgt cttcatccat
    14101 gttgcgggtg acggttaccc tggggaaggg ctcatgccag tggactttga tataggggta
    14161 gcgcttgtca tctttgaggc ggatgttgta tttggggcgg tgcttcttga tgaggttcat
    14221 ttcaagaatc aacgcctcaa gctccgaccc gaccacgatc cactcgatat ccacgatgtc
    14281 gcgcacaagg cgtcgggttt tgttatcgtg gcttgaatcg gcgtggaagt aggaccgtac
    14341 gcgatttttc aggttgatgg ctttgccaac gtaaatgatc ttcccttcaa cattgcgata
    14401 taaataacag cctggctttg agggaagtgt ggcaagaatg ccttgaagtt tttctgaaat
    14461 ttccattggg cgctattata taatacccac ggtcacaaat tgcgctttcc ttcttttgaa
    14521 aatagggcaa tttgtgaccg agtgcggtat aaactcacct tgcatcgaga ggcgtatttt
    14581 tgaatagcat gaccccaatg tcctgtttga aaaagatttc ttttctctta gaattaccga
    14641 aatcaaacat taaggagaat gtaaatggaa ccgtcttttt caaatgcccc cattcagaat
    14701 ggccctactg gtgcgagcgg tggaaatgaa aagatcatgg cgatcgcctc gctggtgatc
    14761 ggtgtgatca atttgtgcgc atggctttta cccatttgtg gaattccact ggctttggtt
    14821 ggggttgtcc tcggttttct gggtatgaag gatgagtcac aaaagatgct tgcgatcgct
    14881 ggtatcgggc tttctgcttt tagcctgttg ctggcctgcg gcaatgccat ctatggtgtt
    14941 tacatcgcta ctcagggtat gtctttctaa ctctgttgta taaaaagcag tcctccgcca
    15001 aattgcggag gactgctttt tttatttagg gaccaggtgt gggggcgggg ggcggcactc
    15061 ggtgtatgcc gagaaggagt ggcatctccg ccatgtaata tgcgtattca taggcggttg
    15121 ttgccccacc ttcaccgaca atgggttcga taagctggtt gttgttcgaa tcaaccccgg
    15181 agatgatctg gtctgagaga gtgaggatct cggtgatgat cggttccatt tccgggccga
    15241 agggcatttc ttgcagccga agcacctttt ccaacacttg ttcggtccag ccttgcatgt
    15301 tttgcaggca gatgactaca tttgcacttt gcaattttat gttggcagtt gaatcggggg
    15361 cacgggcggc aaattgtgca tgtgaaatgc tctgggcaat gtagccctga tcggtatatc
    15421 caggctcgcc gttttgcaga aggccaaacc cgtcaccact gtcactcaca acaccatccc
    15481 catcccagtc tatgtactgg acggtattgg cgttgccagc aatgaggttg gcgatttcct
    15541 ccagcttttg gcgggtgagg gtttcatcgc cagccttgta agcctctccc aattcggtgg
    15601 ttgtggtgtt caggctttct gtggctcccc acaagccctg aattaatgca acaccttcag
    15661 gcgctgtgac gaatgagacc accacatgcc gcacatggat caggctcatg gggggaaaga
    15721 ccgaggatgc cactacattt ccagatgatg tgtctgggtt cgggtcgtta tccacttcca
    15781 gagtgatttc gatctggtcg aacagtgaaa ggacattatt ttgttcaggg ttggtgtaca
    15841 ccaattgacc ttgtgcaagg ctttctttgg tgattgaacc tgccttacgg acaaattccc
    15901 cgccctggcc gaggaaccag acatcgtaat gggtaccttc ctcgggaatg gggagatcat
    15961 cgaatgaaag ggcggcctta tccatcaacc cgttttgatc ggtgaagacc acacggccga
    16021 ggatttcatt gggatcaaca gttggtgttg gcggaggaag agttgcttca ggcgctggag
    16081 tagcctgcgg tgtttgcggt ttgaagaaga ccagtccgag ggctgtgatc gcaaccacac
    16141 cgagtgcaac gccgacccac atgagggttg ggttgttgcg tgttgacttc tttccttctg
    16201 cggcggcttc ggcagcgggt ttgacaaccc tctgtggaat tttggcaata cgggcggtgt
    16261 cggcagaaat ggtctgtccg ttgaacacag ccagaaattc atcggccatt gccttggcag
    16321 agtcatagcg caattcgggg tcttttgaaa gtgcgcggtt gataatggct tgcaggtctg
    16381 atgaaatgcc gaagatgggc ggcggcgggt cattgagatg tttcatgaga atgccgaaag
    16441 atgattcggc atcaaagggc accacacccg ccagcatttc atacaacatc acaccaagcg
    16501 agtagatatc tgttttggag ttgacttttt cgccgcgggc ctgttcaggg ctcatgtagg
    16561 cgggcgttcc agagaccgtt cctgttgagg tttggatgga tgaatctaga aggcggacca
    16621 gcccaaagtc ggtgagtatc ggttccacat ccgcgggtaa gggtttgttg aggtcaatgg
    16681 ggctgcttgg tgagcgcaag agcacattgg cgggtttgat atcccgatgg atgatgtttt
    16741 ggttgtgggc gtaatcaacg gcagaagata gtttggaaag gatgagtgcc acggtttcga
    16801 gcggaagttt ttctccgcgt ttgtgcaggg ctttcaagta attccccatg gatgcgcccg
    16861 tcaccagttc catgatgagg cagggttggc catctacaag ttcataatcg tacacttgaa
    16921 tgatgtttgg gtgacgaagc cccgccacca cccgcgcttc acgttcgaac cgcacgtggt
    16981 tatcaggatc agagtcaaca tggtcgcgca tgatcttgac agccactttt cgattaagtg
    17041 tggtgtgctc gccaagataa acttccgcca tgcccccgcg ggcgatcaat tctccgactt
    17101 gtaccttacc taaagttttt ccattccaac ttggcattga cgcattatac ccgatagtat
    17161 aatgcgaagg attacaagga gattttaatg acacatgcac ttgaagaaac cctgcgcgaa
    17221 catttgcgca cacaaggttt aacagtagtc accgccgagt cctgcacggg cgggttggtt
    17281 tcagatcgta ttaccaatat ctcgggttcg tcggagtatt ttcctggcgg gatcgtggct
    17341 tattcctatg aagccaaagc cagcctgctc ggtgtttcgt gggatacgct caatacgcat
    17401 ggtgcggtca gtgaggaaac cgtgctcgaa atggcgcgtg gtgtgcggaa attgttcaag
    17461 tcaaatatcg gcatttcggt cagcggaatt gctgggccaa cgggcggcac acccgaaaag
    17521 ccagtggggt tgacatggtt cggcctttcc accgacaaag gggaatgggc gcgtcacttt
    17581 atttgggatg gcgaccgcct gcaaaacaaa caccattcct ctgaagccgc tttgcaattt
    17641 gtgctcgatt atctggatgg aaaactggaa tgaaggcggt tgtcattgtt tcagccaatg
    17701 ccgaatggcg cgtggtaaag gaactgtacc cgaacctcga aataaaaaac tcgccttacg
    17761 gcgaatacgc ccacctgaca cttggaacct ggaacctgga actcttccac ggtggttggg
    17821 gcaagatctc tgccgcagca acagcacaat atgtaattga tcatttccag cccgatctgc
    17881 tgatcaacct cggtacctgt ggtggatttg caggccgcat tgaaacgggg acgatcatcc
    17941 tcgtagagcg caccctggtc tatgacatta ttgagcagat gggagacggc gacgaggcga
    18001 ttgaattcta cgcatcttct ctggacctga actggcttcc ctctaattta cctgattcca
    18061 cccgccgcgg actcctcgtc tctgccgacc gtgatattgt ggtggaagat attcccatgc
    18121 tcatcaaaaa atatgatgca gttgccgccg attgggaatc aggggcaatt gcctgggttg
    18181 cacagcggaa taatctccgc gttttgattt tgcgcggtgt gacagatctg gtcggcggtg
    18241 atggcggcga agcctatgga aatctggggt tgttccacga gcggacaaag agcgtgatgg
    18301 gagagttgtt ccgtcaattg cctgattatc taaatttaat aaaaatttga ggtgtagcgc
    18361 gagatactat ctcgcgctac agacattatt atttattttc tcccatcacc gaagtaggct
    18421 ttgttgcagc gttgtttcca tgcaggcttg aaatcaacac atcccccgca tgcaagcgcg
    18481 ggggatgtgt ttttcagagg cagataattt gagtttttac tgggcagggt tcagcacacg
    18541 gcctacaaat aaaatctgtc cgttgaccgt atcgcggatg acaaaaagga agggcctgtc
    18601 tgcgatgaag atgatgtctt gcactggcgc gcccgtcgct tccattacca cggctgtagc
    18661 ggcggcggct tcggtgcctt cctcgtccac tgctacaaag gccttgtgaa tgacatcgcc
    18721 gatgaacagg tctttttcgc ctgtcattcc tgagaaatca gccaaaccac ggtcaaaggc
    18781 gttgaccatg cccagtttga tcagggcatc ggaaaggctg aaatctttgg tgaattcaaa
    18841 cttgggcagg tttacccgcg ccgaaggaac aacctgtaga ccattgacga tctcatcgta
    18901 aacatcctta ttgaaggatg cttcaaaagc ttcgaaattt ccttcctcgg gcaggatgat
    18961 atccatggcg gcggtttcac ctgcataggg aagttcaaca acctgatacc catctccctg
    19021 catgtagggg atgtcgtgta tgttttcgcc catcattttt actgtggctt ctgttccatc
    19081 caaaaggtgg aatgtgctgt cgtatgatga gttggcgtcg aatttgtgaa gccagtctgc
    19141 tttgaagtaa atggcgttga cgagtaccat gcgggtatcg ggggtgatgc ttccttcggc
    19201 gagcaggtct ttgattctgt cttcggtttc agcgctgacc caattgttga tcgccacacg
    19261 ctcttcgttg ggcttgttca aaaaatcagc gaggtggatg cccgccccgt aattaacggc
    19321 aatggtatcg aggtaatcgg gcagaatggg catggttttc tcagcccaaa ccgaattggc
    19381 gatgttgatc tgtaaaggtt cctgatcttt atccaggttg atgggctttt tctccagtgc
    19441 aagatcgaga gcgttgaagg cgggatggag ttgggcctgc ggctggtagt gcatggcctg
    19501 tgccatttgc gattctgtat cgcctcgggc gccagcgtac gtcatggcca gggccaaaga
    19561 catgctgtac ggagagagga tcagatttcc gttttcggag cgaagcgcgt tgtaaatatc
    19621 cagcgcaaag gcgttgttcc catccacaag ggtagacagg tcaccttcgg cgatctgcgg
    19681 atttttgtcg cggcttttgt ttgattcagc cacacttaat gataccgatg gggtgcaggc
    19741 ggccagtatc atgctcagca tgacacataa tgcgatgatt tgaaaagtga ttgatttcat
    19801 ggtttctcct tcgaactaaa gacgaattcg ttttaattat gttcctgctg ttacagcata
    19861 atttccatgg aaacgttgtc catgctttcg cgatagcttg gaatttcgta aaacccgagc
    19921 cgtttgtaaa aatgaatggc gcggatctgt ttatggctgg tttgcaggcg aaggcggcga
    19981 tagccctgtg cgcgggcaaa ctccatcagc cgcatgacca cttgaaaccc gatgcgctgg
    20041 ccgtgatatt cctccaaaag ccagagtctt ttgagttcag ccgtgtctgc gtttattttt
    20101 ttcactgcac ccgtaccgac cactttttct tcgtcgagca ccaccaaaaa caagccgtgg
    20161 ttttcgtgat aggcatgttg aaaatcatcc acatcactta attcaccctc cgcttcgaga
    20221 atatcccaaa aatactgcac ggttttttca ggcatgtaaa ttcgctgagc cacggcggta
    20281 atgaccattt tggcatcttt gatatgttgt gtttgcatga ggtcgatacg aatcatgtcg
    20341 tgattctaac ccgcttcgtg gagcgttata atcccagaac gtcacccata cctcctatga
    20401 tgaacactcc agaacaaaaa gaaattcaaa aaagaaattt tcgttacgtg caaattgatg
    20461 cggtgggtgt aagcattgcc aatgtcgccg cgcctttcct gccagttttt ctcacccgcc
    20521 tcggtgcatc taattttcag gtaggcctgc tcacctccat gccgggtatc accgggttgg
    20581 tgctggccat tcttgtcggg cggtttttgc aaacccgtcc caatattgtg ccatggtaca
    20641 gcgtttcgcg tttgctggtc atttcctgtt atgcgctgac aggcctgctg accttcgtgc
    20701 tttcgaaaga atacgcggtc atatccacgc ttgccatttg ggcctttgcc acacttccac
    20761 aaactgcact ggccgtggca ttctcagttg tgatgaacgc cgtggcgggt cctgaaggcc
    20821 gttatgccct gctcagccgc cgttgggcga ttttcggaat caccaccgtg gtaatgacct
    20881 ttttcgtcac ccgtgtcatt gatctggtgg aatttccgcg caactatgaa atgatgttcc
    20941 tggggttatc agtgggcgga ttcatcagct attacttttc gaagcagatc aaactgcccg
    21001 atcagacccc gccgacgcct gctccatcct catcggtatt tgattcagcc cgaaattatt
    21061 tctccttaat tcgcggcgaa ccagcctttg tatccttcgc attcaagaag tttgtgtacg
    21121 tttctgccat cactttgagc cagcccattc tgccgttgtt tttggtgcgc gaagtgaaag
    21181 ccactgattc acagatcggt accattacaa tggcgatgtc gctggtgatg ctggttgggt
    21241 acttcatctg gccctggtct tcgcgcaaat atggcggacg gtttgtcctg cttgccacca
    21301 ctttcggcat gattctctac cccgcactca cggctgccac cccgcaggtc ccgttgatta
    21361 ttatttacgc gggcatcacc gggctatttc aggcaggtct tgatcttgta ttctttgatg
    21421 agttgatgaa aaccgttccc ccggagcatg gcgccacctt tgtttcgatt gtgcagtcca
    21481 tgcaatattt atctgccatt gccgcaccgt tgatcggcac atggctggcg gatttcattg
    21541 gcttgggcgg cgcgttgtgg gtgagtgccg ggttgaggtt gttgggcttt ttgctttttt
    21601 tgcgcaaaga tacgcgcaga taactttact tccccgtgac gggcatttgg tctttgccga
    21661 tcaaaatggg catatcggcc atgtagtagg catgttgata tgctgtgagc gctccacctt
    21721 caccgttgat gggctcaatg gcttcgttgc cgtttacatc caacccgttg aggatttggt
    21781 tggcaagcgc cacagccttg cggacctctg cttcgacatt ggcatctgca ggggcttgta
    21841 atatcttttc agtcaggtcg cgcagttcaa tggcccattc ttcaatgttg tgaatggaaa
    21901 tctccacatg ctcgttatgc atacggatat ccgctgtaga gtcatcggca tcggcggaat
    21961 agctggagtg atgatgcact ccgcctacat aaccggtctg ttctccattc aagagaatgc
    22021 cataaccatc accggggtcg ttgatcgttc catcaccgtt ccagtctgaa taggcggggt
    22081 cctgcttccc gacgatcagg ttaatgagcg cttctgcatt gttacgcagg tctttttgtt
    22141 tgttttcttc gaaggcaagc aacatggctt ctgcggtctg attgaccaaa acggcattga
    22201 tacgcattcc gtccatcata ccgatctggt tgggtgtttc ttccgtgctg accagcaggt
    22261 gacggatgtg cgccaatgca cggcccggta tgccggtgga gtatttcaca tctcctgatg
    22321 ggttggggct ggtatcgggg atgggttcca gcgtgatttc catgtggctg tacaggctga
    22381 gcaggttgcg gctttgttca tccacgtagg tgaggctgaa ggttccattc tcttcggcgc
    22441 tgaggatgcc aatactgcgc cgttgttctc cgctgtcttc gatcatccat gcttcgtatt
    22501 ggctgccttc tggcagggtt tcgagattgg cgctgatggt gatctggtct gcaatggctg
    22561 ctccatcttg aaagcgcaga atgccaaccg gggtttcggt gttgacgacg ggggcttcgg
    22621 tggccatggt gtggatttct gttgggggaa gggtgggcgc ttctgtggag tttaatctcg
    22681 gcagcacaaa aaggcctgtg ccaacgacac cgcttagcaa aaagaaggaa acgcaggcac
    22741 aaaggaagat tcctgtgccg acccaaagtg gattcagagt ggattttttc ttttgaataa
    22801 cagtagcagg tttgggcggg gtttgagcgc gagtcgcgtg gatggtttcc acttcgaggt
    22861 gtaatccaac tgctttgtaa taatccacca acaggtcgtg tccgctgtta tagcggtctt
    22921 cggggaattt ggtgagggcc ttgtcaatga cagattgcag ctcagggtaa atatcttcga
    22981 tgggcggggg cgcctcgttg atgtgtttga agatgatcgt gattgccgta tcggcttcaa
    23041 aaggaacgcg gcctgcaagc agttcataca ggataatgcc gagtgaataa atatcggtgc
    23101 ggtgatccac ctttgatccg cgggcttgtt ccgggctcat gtaggtgggc gtaccgctga
    23161 cgaatcctga tgcggtttgt tggcctgagt gtgccatgcg ggccaggcca aaatcggtga
    23221 tgatggcttc cacacttttg gtaagcgggg tgtcctgctc aaaatccctg tttctgctgt
    23281 ggaggatgat gtttgcgggt ttgatatcgc gatgaacgat gccctgagaa tgggcatagt
    23341 ccatgcctgc aatgatggat ttgaacaact gaccgatctg ctcaaaggat aatctttgtt
    23401 tatttttgtg aagggtttgg agataggaag caagcgatgg gcctttgagg tattccatca
    23461 cgatataggg atgcccgtca tgtgtgtcga aatcaaatac ctggacaatg ttggaatgac
    23521 gcaggcctgc cactgctttt gcttcacgtt gaaaccgttc caaaagttca gggtcttctt
    23581 ctatgaagct gtgtagaact ttgatggcta cgggacgttc aagtgacagg tgggtaccaa
    23641 gataaacctc cgccatgccc ccccgagcga gatatttatc tacccggact tttccgatgg
    23701 tacggccgat ccattcaggc atgagttgat tatacatctg aatttggagt gttgtagtct
    23761 gaaatgcagg tgaaatgatc gttgatggag gtgtctgggt gttactcaaa gtttgctgta
    23821 atctggctca aagccgccgt cctcggcgaa gggagtaaat ttttaacaca ccccaagtgc
    23881 ctgttcgaaa aattttggag ggggttttgc cttataattc gacgcttaca ggtaaaagaa
    23941 ttttttcaaa cttttttatg aacaaggatg gaggaaacat gtctcaaaat gcgcaaggca
    24001 cccagtctgg cgggcaggag accatcattc aaaggattcg taccagcttg acacaggcgg
    24061 atgaatacac tcgccgtcaa cagcgcctta actccacatt gaccatatcc actacggttg
    24121 ggtctgcgtt gacagctttt gtcactgcgt tgactgccgc tcaaggtcca acggtcattc
    24181 caggtgtttt agattggcag ggcgcttgca ccattggcgc gatcttcagt tttattacga
    24241 cggtctgctc cgggttgaat caacagatga acaccagcca aaaattcctg cttggaagtc
    24301 aatgtgtggg gcggttacgg gccttggaac tggttgcaac agcccaatca cgcgccatgg
    24361 atgaaattac aaatgagtat gcagggatcc tgcgggaata tgccgaagca cttcggaaat
    24421 aggccgcgat ttttataaac accctgtaat gccccataat agaacgtgtt ataattttca
    24481 accgcaactc gaaaatccgt cttgaaagag gagtatatat ggaaattaca atccaggaat
    24541 ttaaacattg tgatttgttt aaagtaaagg gcagggttga tagtgcatct gcaccggaat
    24601 tttccaaagc tctcgaaaaa gccaataatg atggccattt caaaattgct cttgatatga
    24661 gcgaacttga atatatgtcc agcgccgggt tccgcgcttt gctcgccacc cagagaaatt
    24721 gcaaacgtta taatcgcgga gaactggttc tggtaagtgt tcccgagcgc attcgtgaag
    24781 ccttggaatt ggctggtttt accgaactct tcaagacctt caatgactcc attgaagcgg
    24841 ttggcagctt ctaatcgacg cctggggcgt cgcaattaaa aataatcgcc gcctctttgc
    24901 cgaaaggacg gaggcggcta ttaattttta cccatgccaa agatcacttt tccagccagg
    24961 ttcgagtact tggatgaaat ccgcgagttt gtagggcagg ttgcccgcga aggcggtttc
    25021 aacgacaagg aagtttactc cttgcagttg gcttcggatg aagccgcttc caatatcatt
    25081 gaacatgcat atgaaggcgt atcaaacgcc agtttcgatg tcacctgtga cgtgcgtggc
    25141 gatacgatca tcatcaccat gcgtgatgat ggcacgccgt ttgaccccac ccgggtgaag
    25201 cagcccaacc tgaaagcgga tctgtctgag cgtcagatcg gcgggttggg tgtatatctc
    25261 atgcggaaac tcatggatga cgtgaaatat gaatccaatc cgcgcacggg caatttattg
    25321 atcatgacca aacggagggg ataacctcct catgcgttcg tcaactcgta acgggcatcc
    25381 agactcctgg gattggcgtc agcttgcaag tttgggggaa caacttcgca acgaagactc
    25441 cctcagcgca caacgtgacc gcatcgcctc catgaccagt gaccttgttg ctggggtggt
    25501 ggatgtctgg ttaaacgaga gtgtgtttcg cctgccagat tgggaggatg gaagcgtatt
    25561 tcctgagcag ccgcccaaag gcggaatgcg gcttgcgatc aaagctggga aaatcaccac
    25621 ccaaaagacc aatcgaaagc aaaaaaaaga tgcatcccgc aaaacacagc cgcccgcttc
    25681 ccgcgtgact tttgcggctg tcccgcttgc ggatcatggt atcaccatgg gggcttttca
    25741 agtcacccgt tccaaagggc ctgccttcac caccgctgaa ttgaatatgc ttcagggtat
    25801 cggtcagatc gtggcgacca gcctgttcgc ttcccatcgt gccgaggtgg agcagtttcg
    25861 cctcaggcag cttaatctcg tccgcgaggt cagcacgcag atcgcgaatg tgctgaatgt
    25921 ggatgaactt gcggcacgcg tcaccagcct gattcaaaag acattcaatt attactatgt
    25981 tgccattttc accctgaacc cgaattcgaa attccttcgt tttcgttcca gtgcggttgc
    26041 gccgcgcaag ggaaaaaaga aggcttccat tgctcttgaa gtcgaaatcg gccaggggtt
    26101 gatcggcgaa tccgccaaaa gtggtgaaca gatcgtttgt gatgacgttc gttctgatgc
    26161 gcgttaccgc ttcattgaca gcctgcctga gacacaatct gaagtggtca ttccgctcaa
    26221 gatcgaagac cgaatcctcg gcgtgctgga tgtgcaaagt aatcagccgc atgccttcca
    26281 tcccaatgac ctgctgatct tgcatgcgct ggccgataac attgcccgtg ctgtggaagg
    26341 cgcccgtttg tatggcagcc tccgccgccg tgccgaccag ttgacccttg tctctgaagt
    26401 agggcggagt gtcacttcca cgcttgaact atctaaactc atgcgtgatg ccgccactct
    26461 gatccacgaa aaattcaagt acccccatgt ttccctgttc accgtccatc ccaaccgccg
    26521 cttgatcgtg tacgaagcag ggagcggtaa acgcagccac aaactggaag gctttaccat
    26581 ttcattggat gatgccgatg gcatcatgcc ctgggtggcg cggactggcc gcacggttct
    26641 tgcaaatgat gtttccaaac atgaccgcta cctgccttca cctttgccgc caaagaacac
    26701 caggtctgaa ctttgtgttc cgctgatctt caacgacgat gtgctcggct tgctggatat
    26761 tcaatccgat aaattgaatg cctttacgga agacgatcaa atgatgtttg aagccgtggc
    26821 cgataccatg gctgccgcca ttcgcaatgc agatttgtat cgctccgaac aatggcggcg
    26881 tcaggtttct gacagtctgc gcgaagtggc gggtttggtc tccaacaatg taagcgtgga
    26941 tgaagtgttg gaaaccattc tctctgaact ggaccgcaac ctgcccattg atgtcgctgc
    27001 catttggctt ttggaagatg atgacctgtg cctttcggct gtgcatggtt gtgacccgcg
    27061 cctgttggaa agcgtttgca tcaccaatcc cgattccact tatgccatgg ctgaagccct
    27121 gatgtctgat gcgcccatca ttcgccgtgc tgatgagtcg ttgtggcctg ctggcctggc
    27181 attgggctac gagcagaact attcgtccat tgcggctccg cttcgggtgg gtgatcggtc
    27241 tttgggcgtc ttgaccctgt cacatcgcac tgcgggtcgt tacgggcatg aagcacaggc
    27301 catcacaacc acgttcgcaa gttatgcctc ggttgccatt gaaaatgccc gcctgttcga
    27361 tgccgcccag gaacaagcct acgcatcggc ggccttgttg caagtggcgc aggcggtcgt
    27421 cagtttgaac gacctcaacg agattctcgc gtccatcacc cgcatcctgc caattttggt
    27481 gggcgtgcat cgtgtttcgc tgtatcgctg ggattcagag cgggagtcgt tcatcacctc
    27541 gcatgaattc ggtttcactg aaatggaaga aacgaccatg accgagaagg aattcagact
    27601 cggcgatttt ccattgctcg attttgcccg tgagcaaaat tacatggtcg cgcatcctct
    27661 cgacctgggt gctgatcctg cctcctggtt acagattcaa cccgaggcgg agggcgaagc
    27721 ggatcttgtc aactctgagc ggctgttgat ggccgtccct ttatccatca agcatgacct
    27781 gttcggcatt ttgctcgttg aagaagccga aaatggacgc cgcttccgcg cgcggcgtat
    27841 tgagatcatc aatggcattg cacaacaggc ggcgcttgcc attcaaaacg acctgttgca
    27901 acaggagatg gtggtacgcg aacgccttga aaccgaggtg cagcttgccc gccagatcca
    27961 acagacgttt attccgcatg cactgcctgc acatgcagcc tggcaaatgg cggcacgttg
    28021 gcgcacggcg cggcaggtgg gcggtgactt ttacgatgtg atcgaattgc caaacggaaa
    28081 gatcggcctt ttcatcgcgg acgtggccga taaagggatg cccgccgctt tgttcatggc
    28141 gctcacccgc accctggtcc gtgcggctgt gatcgagatg gattcgcctg ccggtgtgct
    28201 gggacgtgtg aacgaccaac ttttgcccga tacccagcaa ggcatgttcg taacggctgt
    28261 gtatggcgtg ttggatgtgg aaagcggtga gtttacttat gtgaatgcgg gacacaaccc
    28321 gccattttgg gtgaatcaga atgggaatgt ggaaaaactc acacgcacag cggttgcgtt
    28381 gggtgtgatg gaacagccca atatggagga gcgtacggtt tgccttggtt ttggcgatac
    28441 cttgctgctc tatacagatg gacttaccga agcattctca ccactgggtg agttgtttgg
    28501 cgatgtgcgt ttgatgacgg cgctgcaggc cattcaagcc agcactgcgg atgaagtcct
    28561 tgccaaggtg gaggaacacc tgaatgaatt catcgagtcc acgcctttgg gcgatgatct
    28621 gaccatgctg gcgttgcgtc gggtgtaaat tcatccctat agtgggggat cgtgtggtgg
    28681 gtgttgagtt atatatttgt tatgtaatca acaaatctgt aaactaaaaa cactcaaatc
    28741 caacttaaaa taactcatca tgtttgatga gctccagact cgtattgcag ataaaaatgt
    28801 gcttctcctt ggggtgggta accgccaacg cgaggatgat ggtgtggggt cttaccttgt
    28861 ccgcaggctg caaaagaaaa ttcgaattcc atcccttgat gcgggcgatg tgcccgaaaa
    28921 ttatattaaa cagatcgaga actccggggc agattttctg ctgatcgtgg atgcagccga
    28981 ttttggcgcc agtccgggcg aaattgccct gcttgagctt ggtgatttga aaaaaatggg
    29041 cggcaccacc cattcggcaa acctgccgtt gttattggag atcatcccaa agaacaaacg
    29101 gccagagacc cttctcgtgg cgatccagcc agggtccacc gggccaggca aagggttgtc
    29161 ggagccggtg cggaattccc tcgacggcct ggatgcgatg ctggtcggga tgtttaaaaa
    29221 ataagacggg ctgcacgccc gtccttttta ttctacaaac ctgtacttga tcagggtcca
    29281 cacggcttca aaggcatcat gtaatttgat cttcttgccc tgcgaatact cacgagggtt
    29341 gaaggtgatc ggcacttcaa agatcctaaa tttgcgtttc aagatcttgg cggtgaattc
    29401 aggctcaaag tcaaaactgt ttgcgtgaat tttcatgcca ttcaacactt cgcggcgaaa
    29461 aaccttgtac ccggtttcca tatccgtgag gatggtgttg tagagaatat tcgtcatcag
    29521 cgtcaacatt tggttggcaa cctgatgcca gaacattgtg acacggtggg cgcggcctaa
    29581 aaagcgcgac ccatagacca catccgcaac gccttcggtg atgggttgaa gcaactgcgg
    29641 gtagtcacgt gggtcatatt cgaggtcggc atcctgaatc aggaggatat cgccttgggc
    29701 ggcagtcatt cctgtgcgga cagctgctcc cttgccttta tttttttcat gcaggatgat
    29761 gtgtacgccg ttctttccat ccaatgtggc taaaacatcg cgcgttccgt ctttggaacc
    29821 atcatccaca acaatgatct cttcggcaag gttctgggct tgcacacgtt ctagaatgac
    29881 cttaatgctt tcgacttcat tgtaaacagg gattatgaca gatagtttca tatgaactgg
    29941 attataaacg aaaagaagta taatcagctg gaaagacaaa ttttttccac cgctgggatt
    30001 gcaagaaaaa tttttatcaa agtctggatt gcgccattat gacaagacaa ccgcaacaaa
    30061 accccgcgcc cgttggcgga ttttccccgc cacagatcaa caaccttgag gatacaggtc
    30121 tttcaaccct ttggcttcag gaccttgtcc tgaaggtttt gtatttccga gggtatctga
    30181 ctggtttcaa gatcgcagaa gagatcacgc tgcccttcgc aggcgtggtg gatcagcttc
    30241 tggccgccct gaaacaggaa aaattcatcg aagtgcggtc atctcaaggc ggcttgggtg
    30301 aaggtgcata tacctatggc attaccgggg cgggcatcat ccgcgctcgt gaagccatgg
    30361 aacgcagtca atacgccggg cccgcccccg tcccctttgc ggtgtataac gaagccattc
    30421 gccgccaaaa gagcggccgc ctcaccgtga ccacccgcac catgcgtcag atcctttcgc
    30481 agatggtgat ctcagaaaac accttccaga ggctcggacc cgccctcaac tcgggttcat
    30541 ccatttttat gtacggcccg cctggaaatg gtaaaaccag cattgcccgt gcctttggca
    30601 accttgtgct cagccaaagc atgtacattc cctatgctct gtacctcgat gggcaggtta
    30661 tcaaagtgta cgatgccgtc agccatactg tggcacccga taacgaatca gggaatggca
    30721 gcggcggaac caccagcaac ctgcgctcca acacccgccg cgacccgcgc tggatcaaaa
    30781 ttcgcagacc gtttatcgta gtcggtggtg aattgactct cgaaggtctc gaccttgtgt
    30841 ttgacgacac cacaaaattc tacgaagcac catttcaggt aaaagccaac ggcggcattt
    30901 tgctcattga cgactttggc cgtcagcagg tccgtccgcg tgacctgctt aaccgttgga
    30961 tcgttccgct cgaaaaccgc gttgattacc tccgcctgca caccggccgc aaagtggaag
    31021 tccccttcga tgtgctcatc gtattctcca ccaacctgcc gccgaaagac cttgtggatg
    31081 aagccttcct ccgccgtctg cgccacaaaa tcgagatcgg cgatccctct tttgaggaat
    31141 accgcgagat cttcaaacgg gttgcccagg ataaacgcgt tgaatacaac gaccagggat
    31201 tggcatatct gctgcaggaa tggtacatca aacgcaaccg taaattgcgc gcctcccacc
    31261 cgcgtgacct ttgcgatcag atcctcgata tttcatccta tcttgccgta cccgccacca
    31321 tgtcgcgtga aatgatcgac cacgccggca aagcctattt tgtggatatc taaccgcatc
    31381 acccctgacc ctgaacctga ttccgatgtt tgaacattat cccagcccaa tcattctcgc
    31441 gcatcgcggc gaccttgccc acgcaccgga aaacaccctt ccatccttcc aacaggcgct
    31501 gcaaaaaggc gccgacggtg tggaactgga tgccaaactc accgccgatg gccacgtgat
    31561 cgtcatccat gacatgaccg tggaccgcac cacaaacggc aaaggtcgag tggcctcgtt
    31621 caaactggaa gacatccgca aactggatgc aggcagttgg tttaacgaga aattccgcgg
    31681 cacccaggtg cccaagctcg aagaggtctt tgaactcatc ggcaaggata aactcatcaa
    31741 cattgagttg accaactact ccaccccgcg tgatgggctt gtcgtaaaag tatgcgaact
    31801 gatcaaacgg cacaacatcc acaagcagat cattttttca tcttttctcc cgtccaattt
    31861 aaagatcgct gctcaaaccc tgcctgaaat tccacgcggc ctgttggcca tgccggggct
    31921 ggttggttta tgggcgcgtt cctttgggtt tatgtttggg gaatatcaag ccctgcatcc
    31981 gcacatttcc agcgcgagtc gtgagcaaat tcagcgtgtg catcgtctca accgccgtgt
    32041 ccatgtttgg acggcaaact cccccgaaga gatcgttcgc ctcaaagact ggggcgtgga
    32101 tggcctgttt accgacgacc cggaaacagc cgtgcgagca cttgggagga gcaggtgagt
    32161 ttgtgcaaaa aacgatttca caagacgccg cccttaaagc ggcgtcagtt ttattttaaa
    32221 agcaagttgt gttataaggg gatgcgatga aaataacccg tattttgttt ttattgaccc
    32281 tgctggtcac gcttgtgcct gttcccgtac aagctcaaac ctccacacct ccggcggcgg
    32341 tctctgccat tttgaattcc atgacccccg aagagcgcgt tggtcagctt tttttggtta
    32401 cgtttactgg tacggatacc agcgtcgact cgcagatcaa tgacctgatc tcccgttacc
    32461 atgtgggtgg tgtggtcttg cttgccgaaa atgataattt tattgccgct cccgataccg
    32521 cctctggaac acatgcgttg attgacggtc tgcaacaatt ggaatggcag gcctccatca
    32581 atccttcaga agatgccact gccggtcagg aagataccga ttccgcctat attccgcttt
    32641 ttgttggcat cgcccaggag gggaatggat atcccaccga ccaaattcta aacggtctca
    32701 cccctctccc aagcgaaatg gccatcgggg ccacctggaa caccgatttt gccatgcgtg
    32761 tcggcgcagt gcgcggacag gaactttcag cgcttggctt taacctatac cttggcccat
    32821 ctttggatgt gctcgaagtg ccagccgtat ccggtggcga ccttgggatt cgtgtttttg
    32881 gcggtgaccc ctattgggtg ggggagatga gccgcgccta tgtgacaggc ctgcatactg
    32941 gcagcgacaa tcaattgctg gtcgttgcaa agcatttccc cggtgtaggc ggctccgatc
    33001 gcctgccaga agaggaagta tccacggttc gaaaatctct tgagcagttg aaacagatcg
    33061 agctttatcc cttctttgca gttaccggca atgccatcac ccccgaatcc actgcagatg
    33121 ggttgctggt ttcccatatt cgttatcaag gatttcaagg aaacatccgc gccaccacgc
    33181 gtcccatcag tttcgatccg caggctttga agcagatcct ggccctgccg caattctcca
    33241 cctggcagac caatggcggc ctgatcatca gcgacgactt gggcacgcaa gctgtcagcg
    33301 agttttatgc atctggtggc gggcagtttt cggcgcgtac cgccgcgcgg gatgccttcc
    33361 tcgccgggaa cgatatgctg tacctgggca acatcacctc cagcgatttt ccagatacgt
    33421 tcaccacgac cacacgcatt attgaattct ttgcacaaaa atatcgtgaa gaccctgctt
    33481 ttgcccaacg cgtggatgaa tcagctgcgc gcattctggc cgtcaaagcg cggctgtatg
    33541 gaaatttcac cctgcaaaat acccttgtac cggcttttcg ccttaccaat atcggcacag
    33601 cagctgaagt gcctttcgaa gtagcgcgaa atgcggccac tctcatcagc ccggagttgc
    33661 aagatctggc agcggtcatg ccgcttccgc ctcaggtcga tgaaagcatg gtctttatta
    33721 ccgatacagc caatatcaaa caatgcaccg actgtttgga acaaccaacc ctggcggtgg
    33781 atgcgttgca acaggcggtc ttgcagcttt acggcccaca gagcggaaat caaacgaata
    33841 attttcgcct cgcttcttat tcgctccaaa atttggagac cctgctcaat ggagaagaaa
    33901 ctcccttcat tgaagatgat cttacccgtg caggctgggt cattttttca ctgactgacg
    33961 ccgcacaagg tcaggcaaac ctgatcagcc gtttcttgcg cgaacgcccc gatctgttgc
    34021 gtgaaaagcg ggtcatcctt tttgcatttg gtgcaccgta ttatttcgat accaccacga
    34081 tctcaaaatt caccgcctac tacgcacttt atagcaaaca accgcaattc gtagatgtgg
    34141 ctgcccgttt attgtttcag gaattatcgc cagtcggcgc ttcccccgtc tcaatttctg
    34201 cggtcggata tgaattgatc tccgtcatgg cgccgaatcc gaatcagatc attccgctct
    34261 ttctggacct gaagcctgtt gccacaggaa cggaatcacc tctcaccccc gagccgactc
    34321 cgattccgct gttcaaaatt ggcgatacca tcgcgatccg cacgggcgta attcaggata
    34381 gaaatgggca tcccgtccct gatggaacgg tcgtgcaatt ctccatgaaa ttgacagggg
    34441 agggtgctgg tattttgcag caagaggatg cggtcactac acaaggtgtg gcccgcgccg
    34501 catttggttt ggataaaccc ggcttgttgg aaattagcgt ggtcagtgaa cctgctgtga
    34561 tctcggaagt ggtgcaattg gatgtttcgc agtctggggc cgtggcagtg acagtggtgg
    34621 taccggagct gaccgaagcc attgagccga caccggaagc tacccccgtt gaagtggtgg
    34681 atgcctacat cagcaaggaa ggctatccgc gtttttcagc ctggttggtg gccatgcttt
    34741 ttatggtatt cagcatctgg gtcgtgtaca gcactgggat ccgcatcata gaccgccatt
    34801 ctgcgttgcg ctggtctttg ggtattgctt tgggcggctt gctggcctac aactaccttg
    34861 cctttggcct gttcaacagc atgagctggc tcacggtcag cgggctttcc ggtgtggttt
    34921 tatttgtatt cattggagaa ttgatggggt ttgtcgcagg ttgggtgtgg tcgcggcgga
    34981 gatgaagttg tgaagttata tggcttaagc cgccgttttt ctggcggctt ttttgttgca
    35041 ccaaaccagt acatttgtag cgatttcact tttcacaaag catgttatga tgatttttaa
    35101 tcactttgct tcaggaggct agttatccat ggcagcccag tcaaccaaaa ataaagaaaa
    35161 gcaaccaccc atcttgttcg ataaaaccca ggcgatcatt aaacaggtaa ataaaatgct
    35221 tggcggcacg ttggtaacct atttcaataa tccgcgcgga agtgtgtgcc acgatgatgt
    35281 ccttgccttg catgaattac tggaaaagat cggccaccag ccgaagattt ttcttttttt
    35341 gaaatccagt ggtggcaatg ggcaggcttc actgcgcctt gtaaacctgt tgcgccaata
    35401 ttgtgatgag gtcatcgcag tgatccccct cgagtgcgcc tccgccgcca ccatgatcac
    35461 ccttggcgcg aatgaaattc aaatggggcc aatggcctac ctcacccctg tggatacttc
    35521 cctgacccac tcactgtcgc cgattgatcg tgacaatgac cgtgtcagcg tcagcctcga
    35581 tgaattgact cgcgttgtcc gcttatggca ggggcaaaat gcagatgcca gggagaatcc
    35641 ctacaaggaa ttattccagc atgtgcatcc gttggtcatc ggcgcagtgg atcgggcaga
    35701 gtcactctcc atcatgctgt gcaaggaact tttggcgtac catatcaagg atgagtccga
    35761 agcggagcgt atcgccagtt ccctgaatgc gaagtatccg tcgcatggtt atcccatttt
    35821 gtttgaagaa gccaagcgca ttgggttgaa ggtcaagcgg cttgaccctg agatcaacat
    35881 actcctgctt gagcttaatg aactgtacag cgaaatgggc cagcgtgcca caaccgattt
    35941 tgatgatact cacgcccatg gcaacgagat tcttaacatc tgggaatcaa ccagtgtgct
    36001 ggtctattac cagcaagata aagattggtt ctaccgcacc gaagaacgcc gctggatctc
    36061 gctcaatgac gtcagccgtt ggcggcgaat gattcaggtg gggaataagg tggaaaaatc
    36121 cattttgcac atctcttgat aaaacctgtg caaaacctgt gcaatctgat agaatctgag
    36181 agtctcagta tggcaaaatt ccgttgctga acaagcgcgg aatttttgca aatattgaaa
    36241 accaaggact ctatggcaaa tcaacctcaa ctaaagttta acgaccctgc acctgacgca
    36301 accctgctcg atgcaaatgg caagcccatc cagctttctt ccctttggaa ggatagggtg
    36361 gtggtgcttg cctttacccg ccatttcgga tgtccgcaat gcaaggaaat ggtggaccaa
    36421 ttgatcgatg cgcatcaagc cctgaccgag aaagggatgc tgctcgcaat catttctcac
    36481 gcctctgcag aatctgccag gcagttctgt gagcagcgtg ctcccagcgc cacctgcctt
    36541 gccgaccctg accgaagtgc ctaccatgct tatggtttgt atcgcggcac cttctggcag
    36601 acattgcttt cgcctcgcat ctggctttca aatcgcaagt tggcacgaac caaaggttat
    36661 aaacctgaat tgccatcccc cggacaggat gcctatcaaa tgtctggtac gttcatcatt
    36721 ggccccgatg gacgtattcg cctgccgtac tactatgaag atattgctga tcacctgcct
    36781 gtggatttga tcctgcacgg cattatgggt gtggattgga aaaggcccta tgacagcaaa
    36841 cctgtggtgt aaatagaatg accagttttt ttctcgctac ctatcttgct ataaaagaag
    36901 tgtttcgcaa ccgtggccgt tttttgctgg tggccctggt gatcgcgttg atcacggttc
    36961 ttgtcctatt cattgccgcg ttgggtgaag ggttgtccga cgccaatcgg caatatgtgg
    37021 ccaaccttga tgcacagttg atcgtctttc tggagaaatc agattacagc atcacagcca
    37081 gcaggttgga aacgaacact gccaaagcgg ttcgccgcgt agatggggtg gcagatgcag
    37141 gcccaatcta tacctccaac tcagagatcg tttcactgcc cgagccgctc aagatttcca
    37201 tgcttggcgt ggaagcaggc cgccccggaa tgccctcact tttggagggg agatatttcc
    37261 gcggtggcgc cgcccgtgaa gcagtgatgg atatgagtgt ggctcagcgc accaatttga
    37321 agatcggtga tgagatcgag atccgttcca cacagggcac tgaagaccag ttttacacat
    37381 tgaagatcgt tgggctggtg gataagcagg cctacttctt ccagccgacc atctttgtcc
    37441 cagcctctac atgggagttg atgcgcccac agtctgaatc ggatctgaac agcgacaccc
    37501 catttccaaa cattgttgcc gtgaagctga ccgatccaac ccaggtcgaa gagatgaagg
    37561 cgcgtctgga agatcaggtg tcaaatatcg aggtggctga tattgagacc accatcaata
    37621 acatccctgg gtacaccgcc cagcaaggga cggtacagac tcagggtgcc ttcaccctgt
    37681 tgatcggcat cctcgtcatc ggcggctttt tccagattca gatcctgcaa aaggttccgc
    37741 agattggcgt tttgaaggcg atcggctctt ccaattttgt ggtggggctt tcagcggtca
    37801 ttcagatcat catcgtcact gccatggggg ttggcattgg cgctggcttg aattacctgt
    37861 tctctttggg gttgccgcca aacattccat tggcctttaa tgggacacgc tcgctcgttg
    37921 cgatcgtcct gctcctgttc attggccctc ttggcggtat ggtatccatc atttatgcgg
    37981 ttcgtatcga gccgcttaag gctcttcggt taggctaggt gcaaggtgtc gaatatagct
    38041 cttgaagttc gtgatctggt gaaatccttt acgcttgatg gcgcaaccat caatgcagtg
    38101 gatcatgttt catttcaggt gaggtctggt gaattcgttg cgttggtcgg ccccagtggt
    38161 tcaggtaaga ccacattgct ttccattctg gcggctttgc ttacccccac cagtggacag
    38221 gttttgatcg acgggcagga ccttgcacag atgaacgaga aaaaacgggt gaaattgcgc
    38281 cgcgaaaaga tcggcttcac ctttcaatcc aacaatttga ttcctttcct gaccgcacag
    38341 gaaaatgtgg aattcatgct gcgcctgaat ggttcgctgg gtcgtgcggg gaaggtccgc
    38401 tccggggaat tgctggcaag actcgggttg gcagaacgtt tgcataacct tcccgctcag
    38461 atgtcaggcg gtcagcagca acgtgtggcg atcgcacgtg ccttgatcca tcaacccgcc
    38521 gtggtgctgg ccgatgaacc cactgcatcg ttggataccg aacgtgcttt tcaggtggtg
    38581 gaaacttttg cccatctcat ccacgaaaac gaacgtgcgg gcatcatggt gactcacgac
    38641 ctgcgcatgt gccagtacgt ggaccgtgtt ctgcaaatgc aggatggcaa actggtgcgc
    38701 gagtattcta cgaaagaaga gattatggaa ctggcgcttg gaactgtgaa gcattaaatt
    38761 tgtgccagcc attcatgaaa aagagacagt cattttttga agtgactgtc tctttttcgt
    38821 ttgttgattt atggcaatgg aatgtttttt gccttcacaa tgaggtacgc gccccaggct
    38881 cgatcaagca attcagaagg acagaacaac ggtttgcctg ttccactgtc tgtcccacag
    38941 gcggggagta atccatcctg atacgcctgc tctgcccaac cgatacccca atagcttgta
    39001 tcggtcatat ctgcaaataa ggtaccagtc cctgctgggg gagtgtaatt tacaccatac
    39061 ttcatgcgta aaccaaagac actagcctca acgcgcggaa gttgtgtggc aggacaatac
    39121 atcaacgggc tgggttggca tccagcagtg agaccctcct gccacatgcc ttcagcccag
    39181 ggttcagccc actcaaaacc agtccagtca tcggcgaagg tatcccacgg ggcgggtgga
    39241 ggggaatagg tggaacccat ttgtccgcgc agcatgaaga cagccgattg agcgcggtcg
    39301 aggatggtat cgggacaata cattaaaggg tcagtgctgc atcctgccgt gtatccagcc
    39361 tgatacaagg cttcgatata gtcatggaga ggatggtcga agggtacatc tgcaaaggtg
    39421 gtggggattc cagcagaaat tgatattgtg tggagcagat tattgaatcc tgtagcagac
    39481 tcccatgtgt ttgcgtttgc caactgtatc gaatactctg gccttgaact cagggaatct
    39541 tccgcgtcag ggaggtttag caaaagttca taactgccag cggggatgtt ggttggtact
    39601 atgatggttt cttgaattgt agtggttgct ccagccgtcc aaaaacgagg gtcgcttttc
    39661 aggggaagat gataaatttc gccagaagag gagtttcgca agatcaattc gccaggtcgt
    39721 ggattgattg gtgcggcgaa tccatcattt ctaatggaaa tatgaacatc cattccactg
    39781 cctgtctgtg ctgtatcggg atatgtgcct tcgataagcg tgaagcgata tcccagtttt
    39841 ttcttcatgg tgtctaaaca cccctgacta ttccaattgg agatggttgt gcccagatag
    39901 tctatattga tatatgacca atgaaaacga tccatctctt ccaatgctgt tgtgcaattt
    39961 gtgcgtggag gtgcccatgt attgttttca cctcccattg ggaggtaggc ggtttcagct
    40021 tccagccagg gatattcctc aggtatattg gtataggttc ccatatcgtc agggctggag
    40081 acaaaagcgt cattaaattg ccctatccgt gatatggaac tgccattgaa tgcctgatct
    40141 ggggtgatgg ctgctgcttg atacatggtg cgtttataga atggtgcgcg caatgaaacc
    40201 atccgcgttg atggcaggac ttgcagcatt ttttcgacga cggcttttcg atctgcccat
    40261 tgttgggctg aaataacacc ctgatcgccg aagtgattgg tgtaatacca ttctccccac
    40321 tgaccgataa aacccgcctg ccagacatag atcacgtcgc tgttcgactg aaggattggc
    40381 gcgagttggt ctatgtgcga caggatttga gaaaggcttg catcaacatt atctgtgctg
    40441 gtgtatgaaa aacgaagcac aaccttcaag cctgcggagc gaattgtgtt caattgtgcc
    40501 tgcagggtgt ttaaggttgc ctggctgatc gcggaagatt taaagctggt gaggtagaag
    40561 ttgcaaagta ccaggctaat atgctcagca gagcggtaat tttgaagagt ggcaaggtcg
    40621 aaaatgtcac tctcacaggg ggaattggta tgtagaaaac ctctttctgg attggtgatt
    40681 atggtgttta cagggtcgta cgttactgtt gcaaaatttg cctgtgctcg tgcaattcct
    40741 gtaatcgaat ggctgggact gatgtttgaa attaccaaca gtgaaactat aagggccaga
    40801 ctgaatttta tgtgaatttt cataagactc tctccatgtg agtaccaata agattgagaa
    40861 taagccacga cacatatttt gttaattcat tatataggaa aacaaaggct cggaaggata
    40921 ccaaaatccc tccgagcctt tgtttaattt gtactacgtt tactacgcgg tttccagata
    40981 cttcgtgatc tcgtaatctg tcacgcgcag gcggaactca tcccactctt cccatttcgc
    41041 gcggaggtag gcctcgaaca ggtattcgcc gagcgctgac ttcaacactt cgttcttttc
    41101 cagttctttc aaggattcgg ccagtgaacc aggcaattcg gtcacaccca gcttggtgcg
    41161 ttcggcctta tcgaggtgat acaggttgat gttgttcaac gccttcggcg ccttcatgtt
    41221 cttgtcaatg ccatcgagag cggcggccag catcacagtg aaagccagat acgggttgga
    41281 ggatgggtcg gggaagcgca actcagcacg gacagccttg tcacggcctt cggtgtagcg
    41341 cggaatgcgg atgagcgccg agcgattctg ctgagcccag ccgatgtaca caggggcttc
    41401 ataaccgggc accaaacgct tgtaggaatt cacggtcggg gctactacgc cagcgagcgc
    41461 gcgggcatgt tccaactgac ccgcgatgaa ggaataagcc agcggagaga ggtgcgcctc
    41521 atccttcgca tcgaagaaca gattatcgcc tgtcttgata tcgaagagag actggtggca
    41581 gtgcatccca gagccattaa tgccgtacac aggcttcggc atgaaagatg cggtcaatcc
    41641 gtgttgggcg gcgatcgctt tcacggtgta cttcaaggtc agcacgttgt cggcggcctt
    41701 gagcgcatct gcatagcgga agtcaatttc atgctggccc aatgcaactt cgtggtgacc
    41761 cacttccaca tcgagtccca tggcatcaag ggcttccatc agtgcggtgc gaacaaccac
    41821 ggcttcatcg aaggatgaga aatcgaaata cccacccgta tcgtggggga cgggatgaac
    41881 gccgcttcca tcagaagcct tgaacaggaa gaactcaggc tcagggccaa tgttgagttt
    41941 ccaaccgcgg tcggcgatct tcttgagaat gcgcttcaac gcgccgcgcg gatcgccatc
    42001 gaagggatcg ccgcctgggg tgtaaatatc acagaacaca cgcgcgcgtt ttgaatctgc
    42061 agccgaccag ggcagcactg cataggtctc tgggtcaatc ttcaggcgca tgtcactttc
    42121 ctgaatgcgc gcaaaacctt ccaccgatga tccatcgaac catgcgccgt ctttcaacac
    42181 atcctcgagg tggcggatcg gcatatccac gctcttgacc gcacccatca catctgtgaa
    42241 ctggagcgaa aggaacttca cattgtcttc ttttacacgt ttgagcagat ctttgttatc
    42301 cattttgttt ctcctttttt gatgtttgac cactgatcat tttctactga tcactgtttt
    42361 ctgattaatg accttgttct gccgtcatca ctttcgcttc gtgcttgacc tttggcttgg
    42421 tactgttaat aaaaatcact gacccgatga tgatggctgt tgccagccag atacgcggct
    42481 caagtgcttc attggcaaac caaaccccaa gtaaaaccgc aacgatcggg ttgacataag
    42541 catacgtcga aacaagcgag atgggtgcat tttgaagcag ccagccatat gaaacaaacc
    42601 cgaccagtga acctataaag ataagatatg ccaatccaac cagcgagcgg gtggatacgg
    42661 ctgaaacatc ccagccattc aactcgcccg aaatcagcga aacgatcaac aggctgacac
    42721 tcccgaccag catttgcgcg cccgtattca tcaacgaaga ttttggcagg tctgctgttt
    42781 tgctgtacag agagcccgtg gcccaaaata gacaagccga cagcagggcg atcacaccca
    42841 acggattcaa cttttcggtg cttcctgaaa tttctgaagg accaacaaga ataaatatac
    42901 ccgcaaaacc gaccaacaga ccaacaattc cctgccaggt gggtttgact ccgttggggc
    42961 gaatggcttc accaattacg agaaacatgg gcatggagcc aatgatgagc gccgcaatgc
    43021 ccgatggaat gaattgctca gcccaggcaa ccagaccatt cccgcccagc aaaaggaatg
    43081 tcccaatgat accagcagaa acccactgct tgcgcgtagg catttggtgg cctgcactgc
    43141 gctgccagac gaccagaatc atcccagaaa taagaaaacg taccgccgca tggaaaaacg
    43201 gagggatggt ctcaatggat actttaattc caagatacgt tgagccccaa acgatgtaaa
    43261 gtgccaataa tggcaaccag attttcgatt tcattttttc ctttttttca tcctgaagga
    43321 atgaagtcct tcgactacat tccttgaaag tcacttgtta gtctgctttg ggcgaattct
    43381 tttgcaagca aagccttctt cctcgcaggg ccgtagcgat aatttccaaa ctcgccgctc
    43441 ttgcgaatca cccgatgaca tgggatcaag accgcgatcg ggttgtgtcc aacggctgtc
    43501 cccacggcac gcagggcatt ggggttgccg atttgtgcgg cgatctgttc gtaggtggtc
    43561 aatgccccag cgggaatttt gagcaatgcc tcccaaacct tgatctggaa attcgtcccg
    43621 cgtagatgaa gattgagagg cgtatcaggt gtcggcgtgg aaaagatgcg gtcaatcagt
    43681 ggggcggtgg ttttgtagtc ttcgatcatc ttcgcctgat tccaatctgc taccaggttg
    43741 tcgatggcat tgccttcact cgattccaca aatcccagat gacaaattcc gcgctcggtg
    43801 gctgcgatca ggcattttcc aaacggtgtg agatgcaagc cataacggat gatgaggccc
    43861 gctccatgcg atttgaactc gccgggagtc actgcttcgg tgttgacgaa gagatcgtgc
    43921 aggcgtccca ggctggagag gcccacctga tgggtggtct ccagtaaatt ctctgatcgg
    43981 gtgagcaggt ctttggcgtt ttccttggtg aggaattgca tgaaacgttt ggggctgatg
    44041 cccgcccagc gggtgaataa acgctggaaa tgatactcgc tcaagccgat ggcagaggcg
    44101 atctcatcca actcgggttg gcggtgaaca ttgatttcga tgtattgaat ggcctgttca
    44161 ataagctgat aatgctgtga aagttcagtt gtggattcca taaattaatc tccttgatgc
    44221 aggagattgt attggtcttt actcacggaa accacccgat tcttgctcat tttctccttg
    44281 ctttcaaaag acaaatcagg ttgatctgta aaataaaaaa cagacatgcc cgggcacacc
    44341 tgtttcaata cgcatgggta ttaaaaaaga aacgtcctct caacaaatac aagtgatcaa
    44401 cgacttatat ccgccacgtc cgctaatcgg gtttcatgta ttttcatgca aaaaattacc
    44461 ccgcttatgc cgtgtatcac caggttttga acctgcattt gcaggtgggt ccctacctag
    44521 aaacgccgcc ttggctcagg cctatcgcgt tatttctaat taagttaagg acccttttag
    44581 tgggtattgg ctcaaaacgt ggaggcaata tcacgaacac agcaggggtg cagttcttat
    44641 accataatca atggtacaag ggtagataat cattaaaccc ccgcgcctgg aaattgattg
    44701 atttaaaata aaaattgtgc taccctctgt atgttgtatc cgaataattt atcgatgacg
    44761 gaggaaagca tggcaacagt tccaggtatt gatgtttcct attgggatgc agggatcgac
    44821 tggccaaaag tgcgggccac aggccagcgg tttatgtttg ccaaggccac cgaaggtatc
    44881 agctataaag acctcacctt tgatgacaat tggttcggcg cgaaatcggc ggggcttttg
    44941 cgcggcgcgt atcatttttt tcgatgtaac gtagatgcga agaaacaggc ggattatttt
    45001 atcgattatg tccgtacagt aaaagacgac ggtgaactcc cacccgtatt ggatctcgag
    45061 accaatgatg gcatgaccaa ggataagatc gtgcctgcgg tcaaggtctg gcttgaccgt
    45121 gttgagtctg cttttggcaa aaagcccatc atttattcag gccaatactt tttgcaggat
    45181 tatgtcgtta tagcgggcgg cggccctccc acctgggcga aggaatatcc gctctggttg
    45241 gcacaatatc ccaatcaata tcaggaaggc atgaaaccct tcctgccgcg cggctggttc
    45301 agttggacct tctggcaata cagcgataag ggcgtggtga atggcatcaa tgccaaagtg
    45361 gatatgaaca tctacaatgg cacacttgag gaattgtata aatttgcagg cgcaaaaatt
    45421 cccgaccaaa ctccaaagac ccacacggtc aaaaagggag acagttttga atccattgcc
    45481 aatagctatg gtgttaccgt ccgtgaactg gtgatggcca acccgcaact tcttgttact
    45541 ggatcgaaat tgaccgtccc tgtggcaatc gccattccac aagaaggcga ttcatcttca
    45601 accgacacag ggtccggcga taccgggaca gtatcaaccc gtacctacac agtgaaggca
    45661 ggtgacacgc tctccgtcat tgccgttaaa tatggaacta ctgtggcaat gatcgcctct
    45721 gcaaacaaca tcacaaatat caacaacatc aaagtgggac aggtgttggt cattccgtaa
    45781 aacgaaaagg taatatataa aaagagctct ggaagaattt tccagagctc ttttgcattt
    45841 gaaaccaacg atctgtttct gctttcttac gggtaaattc tcttgatcgt gcgcgggaag
    45901 ggaattgcct cgcggatgtg ttcgatgccg cacatccatg cggtgacacg ttccgtgccc
    45961 atgccaaagc cagagtgtgg gacggagcca tacttgcgca gctccaaata ccatttgaat
    46021 gcttcttcgg gcaggccttc tttttggatg cgtgccagca acttatcggg gtcgctcatg
    46081 cgctcagatc cgccgcagat ctcgccgtaa ccttcgggag caagcaggtc cacagatttg
    46141 cagacttcgg gtctatcggg ccagggttcc atgtaaaagg ctttgaccgc acttgggtag
    46201 ccgtagacga atacgggctt gtcatagagt ttggtcaatt cggtctcgtg cggggcgccg
    46261 aaatccatac cccattcgaa cttgagtaac tccttgcgtt cggggtcggt ctctttttcg
    46321 tagaggtcga tcaggtattt cgcagcatca tcgtaagaca tgcgcgggaa cgggccgacg
    46381 atattttgca gcttggtcag atcgcgcccg aggaacttca attcggcgga gcaatccttc
    46441 aacactgact gggcgatgtg ggtgatgagt ccctcttcga tgtccatcaa ctgatcgagg
    46501 tcgcaaaaag cgatctcggg ttcgagcatc cagaattcgg ttaagtggcg acgcgtcttg
    46561 gatttttccg cgcggaaggt gggaccgtag caatagactt tcccgaaggc aaaaatattg
    46621 gcctcattgt agagttgccc cgtctgcgcg aggaaggcgg aaccttcgtc gaagtattcg
    46681 gtctcgaaca gggtggtggt cccttcggct gcggcagggg tgaggatggg cgtatcaagg
    46741 ttgaagaagc cctgaccatc caaatactca cgtacagcag acatgacccg cacgcgcacg
    46801 cgcagaattg cccattgact ctgcatccga atccacaggt gacggttgtc cagcgcaaaa
    46861 tccacgccgt gatctttgag cgacatgggg taatcgagat tggcggcctg cacgacctgc
    46921 agatccttaa tgccaacttc aaacccaccg ggaatgccag gggcgcgttt atcttcgcgg
    46981 acggagcctg taataatgac agacgattcc tgtggcaagt gggcgatctg atcgaacaac
    47041 tcgggagtca agtcaccttt gaaggcgaca cactgcacaa agcccgatcc atcgcggacg
    47101 agcaaaaagg ccagtttgcc cttgtctgtg cggttgtaga cccagccctt gaggatgatg
    47161 tcttgtccta catagtttgc tatttttgaa acgctgacgt gttcggtcat gtggtttcct
    47221 tcttttgaca aattttccct attatatttg aaacttcata tatctaggtt acttctttcg
    47281 ttcttccagg ccctttatca tcaattcctt tgctacattc ggg
//
LOCUS       DAOQLC010000015        47158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_209_length_47158_cov_22.321742, whole genome shotgun sequence.
ACCESSION   DAOQLC010000015 DAOQLC010000000
VERSION     DAOQLC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 47158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_209_length_47158_cov_22.321742"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1244
                     /locus_tag="PL000_04320"
     CDS             81..1244
                     /locus_tag="PL000_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC88150.1"
                     /translation="MENLKKYFYLDPSVTFLNHGSFGATPKPVFEDYQNWQLRLEQQP
                     VLFLGREYDQLLFDARAKLGVYLNADPQDLIYVPNATHGVNVVARSLHLEPGDEILTS
                     DHEYGACDYTWDFVCRKLGAKYIHQPIPLPAYTEDEIVDQLWQGVTPRTKVIYLSHIT
                     SPTALRMPVEKISRRAKAAGILTVIDAAHSPGQIHVDLQKVDADFVFGNCHKWMLGAK
                     GSAFLYVRRELQHLIDPLIVSWGYNATPETTTGSRFIDILQWTGTKDPAAALTVPSAI
                     QFMEENNWDEVRKESHDLLRQTLEQICEMVKMESLYPLDSDFYNQMGIAPLPQSNLAT
                     LKSRLYDEYKVEVPLIQWQNRQFVRISVQGYNSQEDLNKLVKALQILLPQVAV"
     gene            1286..2362
                     /locus_tag="PL000_04325"
     CDS             1286..2362
                     /locus_tag="PL000_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC88151.1"
                     /translation="MIVEILNLKKSRSRNLSRWFSFLLFSVLVCAVLLPTPGKAESLP
                     RQTINTPSQLIDAVNNLRIANGLPALAVHSVLMQSSQSQADYMASVGYATHERPGGIS
                     FTQQLLSLGFPLAGDLSLGGFRAENVIQSSNPLVWNGVPPAWQDSQHMNTMLSQNFTH
                     IGAGISQGPSGYFYALDCAAATGSGEMQDGVTTLLTSVPGGAQVGVSQYMVPVMLSTA
                     NANGDVFHKVQYGQTLWSIAIEYGTTIKAIQALNGLGEDLVVYQGQELLVLKGVTPPP
                     AATPTMVQVMETPTSAELITTEMPIPPVTPTSIPQVVTVPATDMPVEEEGSSSNSSGL
                     LVGILVVAAFVGGGVAVWLIRDPN"
     gene            2533..3414
                     /locus_tag="PL000_04330"
     CDS             2533..3414
                     /locus_tag="PL000_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC88152.1"
                     /translation="MEITLALGGGGAKGNSHLGVLRRLLKEGFLIRSVAGTSFGGLVA
                     VLYAIGYSPDGIQEIFETVDQSRLYGRHPEDGPSLLGLAGVRKLLDEVVGQRTFKDLR
                     IPCAVTAVDVKSGCEVTISEGPLKDAILATIALPGIFPAQRINDWELVDGGVLNPVPV
                     SVARMLSPKLPVVAVMLNDPMDKPVPAYTIPMPQILPRSIAERITRMHFAQSLDIFLR
                     AVDVSSRAVAHFRLELDKPDVLIRPNVHDINLLDKVVVSDVIKLGERATEEVLPQLKR
                     AVSWPARVNRRIFGAIQ"
     gene            3411..3638
                     /locus_tag="PL000_04335"
     CDS             3411..3638
                     /locus_tag="PL000_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88153.1"
                     /translation="MSRDLKEYEKQTNRRLIIGAMVLLLVVGVGLIWLIYGGGAASMG
                     FFCLMVGLVPVVLIVGIFYAADWIMKNAGRK"
     gene            3622..4281
                     /locus_tag="PL000_04340"
     CDS             3622..4281
                     /locus_tag="PL000_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88154.1"
                     /translation="MPDANNSTLITFNDWTLRVRESNSDSPRLLVMLHGFTGDENSMW
                     VFARRISTDYHLMAPRAPHPTEPSGFSWRPSQGGTFGRPSLEMLQPAAEALIHLIDEY
                     ADSVKLDASQFDLMGFSQGAAMTNVVGLLYPQRVRKMGVLAGFMPSGLDEQIAQKVLA
                     GRNIFVAHGTQDQMIPVDRARASIELLEQAGAQVTYCEDEVGHKLSANCLSALENYLN
                     N"
     gene            4379..5515
                     /locus_tag="PL000_04345"
     CDS             4379..5515
                     /locus_tag="PL000_04345"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC88155.1"
                     /translation="MKQNFSRRDFLKVAGVGLGALAFKPYFSETYYEKLYTPKRLPQF
                     PAGDIIGRLVDQTDVRSRPDNNPILNNVIGAMPADYLMPWGREVIGSVIGLTNQKYLE
                     TPQGYVWASRVQPTRNLPNTPITEMPAGQPGFWAEVTVPYVDLTLETAPISPWIQSLI
                     QYNFPPRVYYGQVLWIDQVRTTNGFAEYRWNESPGHGYGFGDIYWLDGAGLKVLTDVD
                     VAPISPEIDPNEKKILADLTYQTLSCYEGNNEVFFCRISSGAKFDATGALSEERATPV
                     GELLTHWKIISLNMSGGGSASGYSTPAVPWSTVIHGDGIAIHGAFWHNDFGERRSHGC
                     INVTPEDAKWIFRWTTPYITLAQCETRMTWPDHGTVVTTKELKL"
     gene            5571..6296
                     /locus_tag="PL000_04350"
     CDS             5571..6296
                     /locus_tag="PL000_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNC88156.1"
                     /translation="MEFSQISVGLAFLAGLASFLSPCVFSLVPAYVGYLGGRAAGGES
                     NSKNRWITFSHGLAFVLGFSFVFVFLGVAAAFAGGVLFDLRFWLTKIGGVVVIAFGLH
                     MIGVFHIPFLAYDTRVQQAPDPKMGYLSSALMGVFFSAGWSPCVGPVLGAILTVVFNG
                     GSISQGAVLLTSYSIGLGIPFLFAALGIGWVTNILRNYAKTMRYVEIVMGVILLVVGV
                     MLLTGVFETLALQGQGFYIDFGL"
     gene            6401..7411
                     /locus_tag="PL000_04355"
     CDS             6401..7411
                     /locus_tag="PL000_04355"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNC88157.1"
                     /translation="MLNVTLYTRKNCKLCDEVKHELQELQSKYPHRLVEVDIESDTAL
                     IDKYGIEIPVVEVGPYTLKAPISRQNLQMTLGAAADRKNQLEQVGDPDYQIRVKKGKT
                     VTTGDRVSFWIAKRYLLLLNLFMLFYVGLPLLAPTLMKLGAELPARVIYRIYSPLCHQ
                     FGFRSFFLYGEQAFYPLAEANVAGVKTFEEVTGIPNLDDPYSFTRFEARAYIGDETVG
                     YKVALCERDVAIYLAILLFGITYGLTGRRIKSLHWVLWLLIGIAPIGFDGFSQLFSQF
                     NWTWLATVVPYRESTPFLRALTGALFGLATAWFAYPNIEESMAETRQYYIKKFAVNEA
                     SQ"
     gene            7411..8193
                     /gene="pgeF"
                     /locus_tag="PL000_04360"
     CDS             7411..8193
                     /gene="pgeF"
                     /locus_tag="PL000_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNC88158.1"
                     /translation="MPFQEHDGLRYYRFDIFSNAVTQAVFTRRGGVSPAPWHSLNLGG
                     SVGDDPAHVAANRIRVFQAMGCEPESIHDVWLVHGTDIVYADAPRPLDEPSAKADIIF
                     TDNPRVSLFMRFGDCVPILFHDPKKKVIGIAHAGWMGTLRGVVGAAVDGMRSHYGSDP
                     KDIVAGIGPSIGVDHYEVGAEILAQFREKFNSHADAMFEFREGRIYLDLWKSNSMQLQ
                     QAGVEQIQVSEVCTACHLDDWFSHRAEKGKTGRFGALMALQA"
     gene            8225..8962
                     /locus_tag="PL000_04365"
     CDS             8225..8962
                     /locus_tag="PL000_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC88159.1"
                     /translation="MSDLVKSIRERYLATLDKIASAARSAGRDPDSVRLVVVTKAQPV
                     EVVRAVIEAGAQVLGENYPEESVKKIQYLGQFSAVEWHMIGHVQSRKAQLVAANFNYL
                     HSLDSLKLANRLDRFCSEAGRVLPVLLEFNVGGEESKSGWSAWDEAQWPALRDELGVV
                     MSLPNLKVHGLMTMPPLGETAEFSRPFFQKLKRLQAYLSTQLPKSDFSELSMGTSSDY
                     EVAVQEGATLVRVGTAIVGPRQYKTEV"
     gene            8962..9228
                     /locus_tag="PL000_04370"
     CDS             8962..9228
                     /locus_tag="PL000_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNC88160.1"
                     /translation="MDFQILILLIRNLSTLFIWVVIAYSLLSFFVPPYHPVREALERI
                     VSPFLDPIRRILPAAGPMDFSPVVLILAIDFLSRILIAFLISLS"
     gene            9237..9545
                     /locus_tag="PL000_04375"
     CDS             9237..9545
                     /locus_tag="PL000_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNC88161.1"
                     /translation="MTRKFVLHSGQRGSALAVRITPRASRNQIVGVLSDGTVKVHLAS
                     EPADDVLNVELVGYLSEVLGVPKTRVEVVAGENGRDKLVSILDMDVETAHQRILSHME
                     "
     assembly_gap    9641..9740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9808..12285)
                     /locus_tag="PL000_04380"
     CDS             complement(9808..12285)
                     /locus_tag="PL000_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC88162.1"
                     /translation="MNFTRPSTRRIDSDTRRSRLRTGLRFFLGLWLATAITACSIFPN
                     FPTLPPGWTLTPSTTPTPEATSTPTITPTPLPIARVDKGDHALFNGDYESAMLHYQTA
                     LSDSPDPLVQAAAKWGEARVRFAEERYEETLLSTQELISQFPESPNLGHAYFLQGLAN
                     YRLGNYQAAADSWQTYLVVRPGHLDAHVQELRGDALFEARDFPSALSAYTAAIQASAL
                     GDDTLLDLKVASTQVAIGDYNSALALYDGVFARASNDYIKAQTAYESGLAYQAIGQNE
                     EALGRFRLAVEQYPLSYYSYLSLVALLEAGGTVSQLDRGLVDYFAGQYDVAVAAFNQY
                     LVENPTNDGTAYYYRALSHRDLGNYDAALQDYKKFIEGYPTHPRWVDAWGEKAFIEWY
                     NLGSYTVGIKTLLDFVAAVPGSETSADYLMSAARIYERNGKYEEAIQIWARVANEYPG
                     SEQASTATFLTGIIHYRNGNTVPALDAFNRSLNLSVLAVDQSRAYLWLGKAQQKLGDG
                     EAAVNAWRAAQDRDPGGYYSERARDLLLERQPFQAPISVNLTPNLESERKDADSWVRL
                     TFNLPLETDLSGLGSLASDPRAIRGTEFMELGMYEDARLEFENLRAELETNMDAVGSY
                     RLMNYLVDIGLYRSAIFAARQVLTIAGLDEHTESMMAPPYFSHVRYGLYYSDLVLPDA
                     DSNRFDPLFVFSVIRQESLFEGFVKSSAGARGLMQVIPSTGAQIVSQLGWPVNYTDDD
                     LYRPDVSIAFGTHYLGNNRDLLGGDLYAALAAYNGGPGNAIEWRDLGQGDPDLFLESV
                     RFEETRNYIRNIYEIYTIYRRLYGLSE"
     gene            12409..13554
                     /locus_tag="PL000_04385"
     CDS             12409..13554
                     /locus_tag="PL000_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC88163.1"
                     /translation="MNIHHKIYHFGRLILSAFMLLSIAACGARPQESTEHVTSSGFVC
                     PEPSPRVEFESKEINIFTWTEYVPADMIDCFGLVYGVDVNVDYFSSNEELYTKMSFGE
                     SVNPYDVVHPSDYMIDVLIREGLLQKLDAGKLANTKNLDGNLISAYGNSLDFLVPYQM
                     GTQAIVYNSETVKTPPTSWADLWSPEYEGRIVAVDDNRVVIGMALLTLGYDVNSTDEA
                     QLEEAKQKLIELMPNIRTFDSDSPKTPLVAGDVDLGIVWNGEAFLARQENAAFEYAFP
                     KEGSIIFFDGMGIPTNAPHPDISYAWFNYMMQGDVFWLTLVDYPYTIPNQAALDFAKA
                     NHAEVYEAYMASPITNTPAEIFAQGHKVEDLGNALSLYDKIWTEIKE"
     gene            13564..14229
                     /locus_tag="PL000_04390"
     CDS             13564..14229
                     /locus_tag="PL000_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNC88164.1"
                     /translation="MLNFSPTASEAELRLAIIECGRIAYERHLVTSNDGNISARMLDG
                     NILITPSGISKGRLNPDDMLVVDLEGKVISSRPDRKPSSETPMHLEVYKQRPDVRGVL
                     HAHPIYATTLTVSGLQFPVDVLPEVLLTLGDVPITAYATPSSHEDAEVIRPFVKTHNA
                     MLLCQHGSLTYGKHLDEALIHLERIEHVSEIYWRAKMLGEVKRVPPEAQAKLIELHEK
                     YFS"
     gene            14258..15298
                     /gene="mtnA"
                     /locus_tag="PL000_04395"
     CDS             14258..15298
                     /gene="mtnA"
                     /locus_tag="PL000_04395"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNC88165.1"
                     /translation="MRTVFWENNELKMIDQRILPARFEILSYRGHKEVAHAITDMVVR
                     GAPAIGAAAAFGLALAGYESASTSTEGLLADLRASAAVLKASRPTAVNLAWAVDRLMR
                     VAEQMKDEGNADAVRAAVLTEAQNLADEDVEINKRMAEHGAALINDGDTIIHHCNTGA
                     LATVDWGTALGVIRTAHEQGKRIHVLVDETRPRLQGARLTAWELEQYGIPYEIISDNM
                     SGYFLKAGKAQKVFFGADRVAANGDVANKIGTYMLSLAANDNNVPAYAVVPTSTVDLS
                     LAHGGLIPIEERNTAEVLGIQFQGEPVAPKNAKARNPAFDVTPNRLLSGIVTENGVVY
                     PPFDMNLKKVVK"
     gene            15299..16057
                     /locus_tag="PL000_04400"
     CDS             15299..16057
                     /locus_tag="PL000_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88166.1"
                     /translation="MLFPYSPPTRFDLRFSIAGIPIRVQPLFWLIAVLLGSTSGGIIS
                     LVIWVLVVFISIIVHELGHAFALRRHGISSNILLHASGGLTVPESMRWGGGIASVALT
                     PNQEIFVSLAGPFAGFGLALLVSVLTFIMGGSIVMGTILFVIPVPVHASLPFNLYLLD
                     LFVTDLLWVNIFWGVFNLVPVYPLDGGQVTRYILLIADPWDGLRKSLWLSFIAGIVIA
                     AIAFFLLGSTYMAVLFGFLAFQSYQALQGGLNKL"
     gene            16312..17091
                     /locus_tag="PL000_04405"
     CDS             16312..17091
                     /locus_tag="PL000_04405"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNC88167.1"
                     /translation="MKRVFSMLAGFCLLVLLGGCGPSAEQISTMTASAWTPTSPPTNT
                     PVPTNTPLPTETPAPTAKPPFLFRDDFDGQLADGWVWLGEDTTHWSLAEVPGFMRIIT
                     QGTNIGSDAEPINFLVHAAPAGDFEIETYVQFEPTTNFQFAGLLIYEGQGQALGFGRA
                     FAQCNFPPCKGNALYFDNPTQQNGPNFATPITSPSEVYLRLLREGNTYTAYYSEDASN
                     WTQIGQHTSDITPAFVGLIASQAYEEEKPADFDYFSIRAIP"
     gene            17228..18184
                     /locus_tag="PL000_04410"
     CDS             17228..18184
                     /locus_tag="PL000_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNC88168.1"
                     /translation="MDILLTGSVAYDYLMTFPGLFKEQILPERLASISLSFLVESMSK
                     QRGGIAPNIAYTMTLLGEKPRVMATVGEDFGDYRAWLDSKGVDTELMKVVPGLFTASF
                     FATTDQASAQIASFYPGAMGHSATQSIKELPKKPDLVTVSPSAPDAMMKFPAECRELG
                     IPYLYDPSQQVLRLEGHELARDMEGAHFLFCNDYEFGLISKKTGWSLDQILGHVKVLV
                     ITRGKDGADLYAGGESVHIPTVPEDEIVDPTGVGDAFRGGFLAGYANGFDWKLCGEIG
                     SLSAVYCLEQRGTQNHSYTKQEFIQRFRDHFDDSGRLDALLK"
     gene            18254..19585
                     /gene="ahcY"
                     /locus_tag="PL000_04415"
     CDS             18254..19585
                     /gene="ahcY"
                     /locus_tag="PL000_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC88169.1"
                     /translation="MSNYDIKDIQQAEGGRRRIEWAEREMPVLRSIRERFAKEKPLKG
                     MRLSCCLHVTTETANLMRTLHEGGADIVLTASNPLSTQDDVAAALVNQYEIPVFAIKG
                     EDKVTYFKHIRAALEHKPHMTQDDGADLVSSLFFIEMGRFERLEPSLAEWAKTLSEEE
                     RKQMLKNVIGGTEETTTGVIRLKAMEKDGVLKFPVIAVNDALTKHMFDNRYGTGQSTI
                     DGIIRATNVLLAGKTFVVAGYGWCGRGLASRARGMGAHVIVTEIDPMKALEAVMDGFQ
                     VMPMFDAAKVGDIFCTVTGDLNVIDGKHFEVMKDGALVANSGHFNVEINIPALEKMSV
                     GAPNLVRPFVDQYLTKDGRKINILGEGRLINLASAEGHPASVMDMSFANQALSAEYMA
                     KNADKLEKKVYSVPTEIDNEIARLKLEAMGVKIDILSDEQLHYLNSWEEGT"
     gene            19675..20505
                     /locus_tag="PL000_04420"
     CDS             19675..20505
                     /locus_tag="PL000_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88170.1"
                     /translation="MHARINHIHFVEDSQKSPVVFVHGFPDSPAMFAAYHTEAERRQA
                     WLQGRSIYAYAFPNRHDNPNFPSLGELAGGVMDREFNALMDELARNSPTGKLVIVAHD
                     WGATHTWRWAREKKHPPIEKLIALSVGSSFRYDVFEHGLNAFTWLYGLWFCAAWYLPF
                     LRKTVAASIVSRAGYHSETAHELWKDAYHYWDRPSLLITIFLQALFFLFYKPEYLDFD
                     FPILYMRTKLDRIASTARFEQALRTRPDARFVLFEQFNHWFPEQHSDLVLAEIRSFVD
                     "
     gene            20559..20828
                     /locus_tag="PL000_04425"
     CDS             20559..20828
                     /locus_tag="PL000_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88171.1"
                     /translation="MDKKIKPSEVIAARFQVSNETAKFFLGRVQKSFKTERPPHQLVV
                     DFMSGQSYDALPKPYQIAKALNEKGIWAYPLNSEPAEPVDEDELY"
     gene            complement(20825..21655)
                     /gene="rsmA"
                     /locus_tag="PL000_04430"
     CDS             complement(20825..21655)
                     /gene="rsmA"
                     /locus_tag="PL000_04430"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNC88172.1"
                     /translation="MTDYRSIPPLDAHAVLKRFQLRADKSLGQNFLQDSSALEKIARA
                     AEIQGDDCVLEIGPGLGSLTRYLAASAQKVTAVELDPDLLAPLRAVLSPYQNVNVIHG
                     DILKLNISEIIEQPDYLVAANIPYNITSAIIRHLLESTPKPRRVVLTIQKEVAERICA
                     APGDLSLLALSVQVYGRPSIAAKIPAGAFHPVPKVDSAILRIDIHKEPLIPAELLNTF
                     FKLIKAGFSQKRKTLRNSLSSGLHIPPHEAESMLTAAGIDFMRRAETLSIEEWRRLCG
                     "
     gene            complement(21652..22779)
                     /locus_tag="PL000_04435"
     CDS             complement(21652..22779)
                     /locus_tag="PL000_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC88173.1"
                     /translation="MTQSQKRILAILSFVAFVLVGTAVFFAFRSYRAFVTQPLGPSLP
                     IVAQTMPPTWTPAATTSPNMITLAPTLSFTTPTPYAGCGGTTSVMHVVAIGADSRSDN
                     YQYGLGDVIRLVRIDFVTPKVSVLEFPRDLWVEIPYISDNLNGQDHEKLNQAYLYGQP
                     GDGFHYWDDPSAGPGLLSLTINLNFGAQVDHYVAVNMRTFVNVVNAVEGIDINIPDET
                     AAENTGLTIGKHHLNGDQALKVARNREEGVFERASNQNRVLCALRQKLASPAVVTKIP
                     ELIDSFQDNILTDFTPEQIAQLACLGTKLPPENIVFASFPQELFKESRIYDPVFKKNV
                     FYWDVDFNILRDYTAKFQAGTWPEPNTITTDEEDEEEIICE"
     gene            complement(22891..24108)
                     /locus_tag="PL000_04440"
     CDS             complement(22891..24108)
                     /locus_tag="PL000_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNC88174.1"
                     /translation="MKFLRWLALAATMLLSACQSNPSPSITIIDSGQIQVVASDSRIP
                     LVLLTQNGITPQQFDRVLVNGVPFPIDQPLPSVDSVQLQLRRAVDLTLTTPQGTETFQ
                     TSALNVGQALAEVGYQIGAADVLDPPAETPITTAMNVTFTPARDLTVQVGESVVHIRS
                     AAGTVGEALAKAGIPLIGLDTSFPLSGEALPADGQIQVVRVQEIIDVAIEPIPFETKK
                     ISSIDVPLGQEELVQSGVNGIAMTRTRIRYENGQEVSRILEEEIVAREPQPRIVKSGE
                     QIVMSPLPGVSGEYWTAVEMYATVYSPCESGTGGCSYSTASGARAGYGIVAVDYSIYS
                     YLAGMRVYIPGYGTATIGDTGGGPIIETAFGVPRTKWIDLGFDDGAIQDMTGWVTVYF
                     LAPAPAEVPYFLK"
     gene            complement(24135..24797)
                     /locus_tag="PL000_04445"
     CDS             complement(24135..24797)
                     /locus_tag="PL000_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNC88175.1"
                     /translation="MNTLVQKSNTAVYWQYALWLAIFTVFYNIAEGLVSISFGVSDEA
                     LTLFGFGVDSFIEVISGAGILAMVLRIRQNPNTSRTRFEVTALRITGTSFYLLAVGLG
                     VSAIYNLVIGHKPDTTLPGIVISVISIAVMWALVMGKRKTGRMLNSMPILADANCTMV
                     CIYMSVILLISSLVYAVSGFGFIDSIGALGLIYFSYKEGREAFEKANGLECTCEDEHC
                     KD"
     gene            24935..25333
                     /locus_tag="PL000_04450"
     CDS             24935..25333
                     /locus_tag="PL000_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HNC88176.1"
                     /translation="MKKIVVVFLVLVFLLTACGGNKPSKNLRVDMLEFTFTPAEFVVP
                     AGEEITITVANNGAVLHEFVIMKFGQTVGDDFGDEDEGNIYWEVEVEPGKTVTTTFTA
                     PADSGEYQVVCGTAGHFMAGMAGTLVVAAP"
     gene            25402..26172
                     /locus_tag="PL000_04455"
     CDS             25402..26172
                     /locus_tag="PL000_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC88177.1"
                     /translation="MEMITSLANPLVKHARALRQKKTRLETGLFLVEGIHHVGEAIEA
                     DWKIETVLYAPDLLTSSFARNLLTRLKTPPQPVSVAVMESLADKENPQGIIALVRSRQ
                     TQLTDLKKFSSAVALVSPQDPGNVGTILRTMDAVGAEILFLLEGGVELYHPTVVRASM
                     GTLFWKPIVQASFDDFIAWARKGGYQLIGTSAKADVDHHTLTPHTPWILMLGNEQKGL
                     TTEQSAACDVTVSLPMKGRVSSLNLAVAAGVLLYQFGE"
     gene            complement(26200..26577)
                     /locus_tag="PL000_04460"
     CDS             complement(26200..26577)
                     /locus_tag="PL000_04460"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC88178.1"
                     /translation="METTKSKFASIDEYIATFPKETQKLLNDLRAAIKASAPNAEEKF
                     SYGIPTFFLNGNLVHFAAFKTHIGFYPAPSGIEAFKKELSMYKQAKGSVQFPLDAPLP
                     LKLVGKIVKFRVAENLKKTSKKK"
     gene            complement(26650..27132)
                     /locus_tag="PL000_04465"
     CDS             complement(26650..27132)
                     /locus_tag="PL000_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88179.1"
                     /translation="MSRKTIIYSISVIMICTGLVQIWRFFSPLFGAGKSMNLTLILSG
                     AFVLSAGWNLLKLNTTGRELAFWILFMNLLGGLLVLGFILPVNGIVSINVQFLDKPVF
                     NSVDERLFSIVFLAVFLVIDLSALVFLEQEKTKKLFAPDLVENLTSKADAESKSTSEK
                     "
     gene            complement(27327..27560)
                     /locus_tag="PL000_04470"
     CDS             complement(27327..27560)
                     /locus_tag="PL000_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88180.1"
                     /translation="MPRIKCHYIDCVFVDEGYCSAAAVEFDPDTGCNTYSAADGVAPK
                     DDDWDEEDETEEWDDDAEEEEDDDWLDDEEDEF"
     gene            complement(27572..28000)
                     /locus_tag="PL000_04475"
     CDS             complement(27572..28000)
                     /locus_tag="PL000_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC88181.1"
                     /translation="MDKWLYRASVALVVLGLLVSIYMTIYKYSGNDNMCLGSGDCHTV
                     NASRYSEVNGIPVAVFGIGGYLAILAVLYFENRNRFFRENATLMVFGMALTGFLFTVW
                     LIYVEIALLKAICPFCVTSQVSMTLIFILAVWRLIRQPQS"
     gene            complement(28007..29230)
                     /locus_tag="PL000_04480"
     CDS             complement(28007..29230)
                     /locus_tag="PL000_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNC88182.1"
                     /translation="MYSVIQARERILSHFESTPHESVPLVACSGRVLAVDVTAAHDLP
                     PFDNSSMDGFAIRSADSANAAVQGLTLSVVADIPAGSAPKVTLATGQAARIMTGAQLP
                     AGADAVIPVEDTDFHVRDAGTTPPETVSFSRKVIAGENIRPRGMDLHAGDVVLKKGRK
                     LKPQDLGLLAMLGIAKVDVHKKPRVALLSSGDELLEVDSPLSEGKIHDSNSYALAAAF
                     ESVGAEVIRLGVAKDTRESVESLLKKSTLEHADLIVSSAGVSVGAFDFVKDVIETNGK
                     LDFWRVNMRPGKPLAFGSYKGLPFIGLPGNPVSAFVGFEVFVRPALEKLSGLLDGTRL
                     TVKVRCGEVIESDGRESYLRANIRLENGIQTAFLTGHQGSGNLLSLVQADALLIIPAG
                     VKCVPVGQEVEALLL"
     gene            complement(29273..31030)
                     /locus_tag="PL000_04485"
     CDS             complement(29273..31030)
                     /locus_tag="PL000_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88183.1"
                     /translation="MKRQKFNPVTMTILLLVFASVAFRVQAIPNSNRDMAVYNLRWYQ
                     EISQKGIGNALGTNFSNYAPPYTYLLALATLTRDLIPPLTAIKLIPIFFDLLGAYFIY
                     RIVRLKYPQGNLSMLAAAIYFSAPTVVLNSAYWGQADSIYTSALLACLYFLLTEKPLP
                     AMLAFGAAFSVKAQAVFLLPFLGVMVLRKKIRWWVMGVIPLVYLLAIAPVVLLGRPLM
                     DALLVYAKQSDTFNMLSMNAPNIYSLFQREWYTVVLPFGLVLTAALTLVWMYSSWQAK
                     TDLDDKHIVLLAFVSAMLIPFLLPKMHDRYFYPADVLSIVLAFYWPALWFMPILLQIS
                     SGGAIAVFLFGADPAFTVYGFLLNSIALTIAMHTQRVSEKRQTHPLVSSSLAWLAAFL
                     VPLALFGIGARLLLTPTFIRAEYALPYTQEAQGLSRSERFQQASQTLEYMGSDRRPQF
                     LQRLRLDNGAPVFTEQEITTIESVKQSLRTTFSIWSLSLAVLFLLTLLAQAGEWVSKF
                     RHGVQRGGWITVAVAVISAAAVIFVPIKVFEGEMLSRLFPNTIWRDAVLILCLFMAAS
                     GFLLARSLSTSGGNTASSQ"
     gene            complement(31032..32348)
                     /locus_tag="PL000_04490"
     CDS             complement(31032..32348)
                     /locus_tag="PL000_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88184.1"
                     /translation="MKSNFWEKSADILRRNAALFAFLLALGLYGLSAGIKLTQQSKTP
                     QYVYLAYSFLQGHTDLVELPESRYDLIEFKGKWYVPGGITPALLSLPFVILFGKGFSD
                     VLFGVLVGATNAALMAVLLGQIVEDFSTRIWLIALFIAGTVHWWLASVGSVWFNAQIV
                     ALLFMLLYARDAIRNRPWLAGLWLGLAFLARPPTIFSALFFLLLVVLRERSITPVLKK
                     MIPFGVMLAASVAVMLTYNQLRFGNPVDFGYGYVRGSNALIQTFARSGGFNVQYMPCN
                     IHVSIFGLPNLNTGIFPSVNEACSYLEPISHDFGKLSKFFNPLGMSIFLATPAFLLIF
                     RAKRRDDWVIPAWAGMIGILIPLWMYHATGWVQFGYRYTTDFIVFLFILLASAIKEIR
                     WMEKSLIGMSVFMGGTGMYLMYYMTFGLVWPEMFMELARKIYHFIF"
     gene            complement(32370..34601)
                     /locus_tag="PL000_04495"
     CDS             complement(32370..34601)
                     /locus_tag="PL000_04495"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC88185.1"
                     /translation="MKSSNTAIPLEKLIEQLPEQYTVADRELVQRAYRVAEEAHREQK
                     RNSGEPYINHCIAVASILSDLKVPPEVIAAGLLHDTVEDTTITLADIRRDFGDTIKIL
                     VDGVTKLTHLPRVSRGDQHPEKDENGNHTEEKPIEPALLGRKEDIVSETLRKTFLAMG
                     DDVRVILIKLADRLHNMRTLGHMPEHKRRRIAQETLDIFAPLANRLGIWQIKWELEDL
                     GFRYVNPEKYKEIAELLTEKRPDREAQLEAIKENLVKLLEKNNIKAEVSGRPKHIYSI
                     FKKMQKKGKPFDMVRDVRAVRLIVPDMPSCYAALGVIHTTWRPIPGEFDDYIAAPKDN
                     FYQSLHTAVIYDDKRPLEVQIRTNEMHLNAEYGIAAHWRYKEGTKHADRNYEQRINSL
                     RTMMEWRSDVKDAAEFVESMRSDVFQDRVYIFTPRGDIIDLPAGSTPIDFAYHVHTDI
                     GHCCRGARVNGKLVPLYQELKTGDQVEILTAKRGAPSRDWLNPNLGLVRTQRARSKIK
                     VWFKKQEDEQNLAQGRDALEKELQRLGLQDINFERMARDLEYKTPDEMFIALGNGDLS
                     VSKVIKQFSQQEAASDIFEVNPSTTPSTSTNAIEVVGLKGMLSTMARCCNPMPGDQVV
                     GFITRGRGATIHRQDCPNILILQTKERERLLQVGWGHVERTYPVPIKIKAYDRQGLVS
                     DISNLLADENINIADVKVNVNRSMADLRLIIEVKDLTQLSRVLTRIENLPNVMEAHRV
                     NSG"
     gene            complement(34701..35153)
                     /locus_tag="PL000_04500"
     CDS             complement(34701..35153)
                     /locus_tag="PL000_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC88186.1"
                     /translation="MTTASVLSQFGLFQDLPQQLLEEIAAISDEVEMKQGEFVFREGE
                     KADKLHFLLNGSVALRVKLTSRPESLTVSFVSTPYQSFGWSGIVAPNHYTSSAECDED
                     SKLLITPAQPFLKLLEKYPEAGFKVMQRIAEIIADRLRNSRQALIKTL"
     gene            35288..35764
                     /locus_tag="PL000_04505"
     CDS             35288..35764
                     /locus_tag="PL000_04505"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HNC88187.1"
                     /translation="MTVTNNVTRFLDARKVKYEAHELPPEKLGAVEAAQHMGVPPEQV
                     FKTIVVTREKKKPLLTVIPGPRVVDLKKLAAFLNEKKVSLPTEREAEALTGLQAGGIS
                     PLALINKGFQVVIDSAAQGFEKIYISGGQRGLDIQLPVADLASLTNAKFGDVSRDE"
     gene            complement(35870..36772)
                     /locus_tag="PL000_04510"
     CDS             complement(35870..36772)
                     /locus_tag="PL000_04510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNC88188.1"
                     /translation="MTKLITLKQAISDFVKDGDVVYAAGFTHLIPFAAGHEIIRQGRK
                     NLTLARATPDLIYDQMVAAGCAKKVIFSYMGNPGVGSLRIVRAALEKGELEWEEYSHF
                     GMITRLQAGAAGLPFLPMNQTGATDLERSNPLIKRIPDPYGGKDVIVVPALNPDVAIV
                     HVQRADKNGNAHLWGIIGEQKEAAFAAKKVILTAEEIVEESVIRSDPNRTMIPANIVD
                     AVCHVPFASHPSYSQGYYDRDNEFYLAWDKISESQDSVKAYLDEWVFGVKDREEYWQK
                     LGSEKREQLKVVSALSEPVNYGKY"
     gene            complement(36774..37037)
                     /locus_tag="PL000_04515"
     CDS             complement(36774..37037)
                     /locus_tag="PL000_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88189.1"
                     /translation="MDDDFVFRIGMFFYVIGGGSFVLFVTSDIADKVDFDFLFVALLM
                     FGIGAYLRRGMAPPPSAGRFASFKKWRENAKNKKQQKQEAKKK"
     gene            complement(37062..37814)
                     /locus_tag="PL000_04520"
     CDS             complement(37062..37814)
                     /locus_tag="PL000_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC88190.1"
                     /translation="MPWDPTQYHKFQAERSAPFFDLLKLVNIRPNLQAVDLGCGTGEL
                     TKHLTEALPEGTVLGLDSSLEMLNKARQHESPSLRFEQGDQSELQGDWDLIFSNAALQ
                     WSENHEALIPRLFGRLKENGQLAVQVPSNHFHISHQIIRETAREEPFQTALGGFNRQA
                     PVLSIERYAEILFECGAKDITVFEKVYPHVLENSDAIVEWIRGTALVPYLERLGQQKE
                     AFTEAVRTKLKSAVPGSPVFYPFKRTFLSAVK"
     gene            complement(37885..39459)
                     /locus_tag="PL000_04525"
     CDS             complement(37885..39459)
                     /locus_tag="PL000_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC88191.1"
                     /translation="MADFKLTYATMFNPPEELHTGFDKAVEKVKKNMGQEYTMLINGK
                     EVKADDKFDDFSPVNTDWKLAVMQKGNAKHAQEALAAARAAFPMWSHTPWQKRVELLR
                     KAASLIEERIFDLGAAMALEVGKNRMESLGDVQETADLIYYSCYQMEKNDGYIVEMGK
                     DPLVGYDARNVSVLRPYGVWLIVSPFNFPFALTGGPAGAALVTGNTIVIKPATDTAWI
                     VRLLVDCFRDAGIPDGVVNFVTGPGSTLGQALVDSSEVDGVTFTGSFDVGMKMFQDFG
                     KRNYVRPIILELGGKNPAIVSKNADLERATIGIIRSAFGLQGQKCSAASRVLVEDSVY
                     DDLVGRLKEAASKLVLGDPTDRNTYNGPVVNKSSYNDFKNFCEEINQGGGKFLVGGNV
                     KTGGMFDKGYYCESTFVVDLPFSHRLWKHEMFLPITTIGRVKNLDEAMSIANDVNYGL
                     TAGFYGSTEETEWFFDKIEAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNAGGH
                     YYLPLYMHEQIRTLVK"
     gene            39684..40016
                     /locus_tag="PL000_04530"
     CDS             39684..40016
                     /locus_tag="PL000_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88192.1"
                     /translation="MRPHFPDRIQRHGSFLTVVLVAVFAASACAPAAATNPPPAAELP
                     TATTAPAADAIPTATGEVAAPVVPTQAVAEVLPVATSRGPNLEATDPTTVNLAAGQLQ
                     LVEFFRFT"
     gene            40032..40256
                     /locus_tag="PL000_04535"
     CDS             40032..40256
                     /locus_tag="PL000_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88193.1"
                     /translation="MAPMVHGLEAEYFGKVKFSYLDADDPRTDGFQRALGFYYQPELY
                     LLDANGNVLKKFIGLTSREDLVAEFEKYLQ"
     gene            40365..40997
                     /locus_tag="PL000_04540"
     CDS             40365..40997
                     /locus_tag="PL000_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88194.1"
                     /translation="MRIGKSSLFLVFALVVLTLPTACNLGVVQPEASPPPVAIVETPT
                     FVIPPPVEPTATPTLIPTIVPTATVTLFHSSIPTLTPTSQWTGCPGIVVKVDDTKKGD
                     ILNILRCDDGLEYQLGPLAKGVYAVGPNNNFLIYVSFDGIVYAARIGDRYLNPLYNLG
                     REHIFTVFNKKVAPDFQLSFTGEGPIYRLVILEKNYDQKRMYELPIGLTE"
     gene            40998..42095
                     /locus_tag="PL000_04545"
     CDS             40998..42095
                     /locus_tag="PL000_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88195.1"
                     /translation="MGIFIKVFVFIAGLVVVALTVFSALSTFVLPRAARSQLNRLVFG
                     VMRRVFEFILRFFPTYEQRDSIMAYYSILGVMMLVPVWYFLVAAGYAAMYWSLGVGDL
                     YADFRFSGSSVFTLGFATSDNPIVTFLSFSEALIGLVLVALLMAYLPTMYAAFSRREQ
                     AVNMLEVRAGNPPNPYDMLSRFHRIHGLDKLGDLWKNWEIWFADLEESHTTLPALVFF
                     RSPRADNSWVTSAGAVLDTAALTLSSIDIPNEMSAALCIRAGFIAFRRIADYFDITHP
                     QNPTYPETPISVSRAEYDEVIRKLEAAGVPIKPDREKAWRDFAGWRVNYDRVLLVLCT
                     LVMAPQVPWSSDRAPKFKMPPLFVPKKKLKH"
     gene            complement(42154..43512)
                     /locus_tag="PL000_04550"
     CDS             complement(42154..43512)
                     /locus_tag="PL000_04550"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88196.1"
                     /translation="MAKEGAMVNDRREIFGWAMYDWANSAFSTTIGTVFLGPYVAGLA
                     RSAAEAAGTTNVSFLGIPVAPDSFLPYCISFSVGMQVLFLPILGAIADYSHLRKRMMQ
                     VFATIGAIATLLMFLVTGGLWWLGGLLFIVANLAFGAAIVFYNAYLPDIASPDETDRV
                     SSYGWAMGYMGGGILLALNLAFFTFAEDLGVPSELAVRINLASAGIWWLGFSLITWSR
                     LRPRHAARQLPAGDTYVSIGFKQLRKTFSEVKHFPETLKFLLAYFLYNDGIQTVIAVA
                     STFAAAPLIQGGLELEQSTLIAVILMIQFVAFFGALFWGKLAGWIGAKQSVVVSLVIW
                     AGVVIYAYGGLKGESATAQFFVLGVFIAIVMGGSQAISRSLFAQMIPNGKEAEFFSFY
                     EISERGTSWIGPLVFGLANQIFGNLRYAILSLIFFFIMGLILLPFVNVSKAVEDAKKY
                     EG"
     gene            43676..45007
                     /gene="ffh"
                     /locus_tag="PL000_04555"
     CDS             43676..45007
                     /gene="ffh"
                     /locus_tag="PL000_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNC88197.1"
                     /translation="MFETLTGRLNQIFDNLRRRGKLSEADVDAAMKEVRLALIEADVH
                     FSVVKTFIARVRERAVGAEVSKALNPGQQVIKIVNEELIASLGEPVGLNLTGPKPRVI
                     MMVGLQGAGKTTASGKLARLMKAKGERVLLVAGDPYRPAAVQQLQQLGERLDVEVEAD
                     LSLTPPQLAKRAVDKAEKGGFSIVIVDTAGRSQMDAALMDELKAIAGNINPVDILLVV
                     DSMIGQEALNIAQGFKDAINLTGLILTKMDGDSRGGAAISIRSVTGVPIKFIGTGEKL
                     DALETYDPSRLSSRILGMGDLIGLIEKAEAAYDAKDMDAQARQAEKMMKGHFSLEDWL
                     DQMKQMKKMGPLAQIMEMLPGQMGQAARGIDPSLVEKSFKQSEAIINSMTLKERRNPD
                     ILNASRRRRIAAGAGMEVQDVNRLIKQFREAQKMMKMFQKTGGKGLGKLFG"
     assembly_gap    45057..45156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(45191..46186)
                     /locus_tag="PL000_04560"
     CDS             complement(45191..46186)
                     /locus_tag="PL000_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88198.1"
                     /translation="MKTKNPTAIFSIILILSVLACNLPGSATPQATPVPILPTETPAA
                     SASATPTTVPTETEIPSPTVVPPTPTIAVFHSLVPATNIRAGDLIHDVVSVDTAAENR
                     APYGDSYNINLFERPFTQDMTYIPDVDIVSYNLSHDEKFYYVSIELVGKNPNNEIGIN
                     YAVELDLDADGFGDYIIAARPPYNAEWSTDGVVVAKDSNRDTGGLSAEKSDAPYPGDG
                     YNEIIFDSGLGSVNDSDLAWVRINAGRYATVQFAFKLWVAENKFMYGVLADAGPRSIA
                     DLDYVDRYTEEEAGSPVRSDKFYPLKALFGVDNVCREVQGFTGTGQEPQRCLPKK"
     gene            46306..47073
                     /locus_tag="PL000_04565"
     CDS             46306..47073
                     /locus_tag="PL000_04565"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNC88199.1"
                     /translation="MDYSSAELMIINAARLLRDGDVVFVGVGQPNLACNLAKRTHAPN
                     LVMVYEAGVIGAEPARLPLSIGDPTLVSGSVSVVSMYDIFANYLQRGNVDVGFMGGAQ
                     IDKHGNINATVIGGYDHPKVRLPGSGGSQEIAAWANRCYIMTPHQKRRFPEKVEFMTS
                     AGFIGGKGDREKAGLRGKGMVGVVTDIGMLEPDENGEMVLTALHPGKTVEDAKANTGW
                     DLKVAAEVRVTEPVTENELRILREELDPTGIYLKSGG"
BASE COUNT        11074 a        11902 c        12405 g        11577 t
ORIGIN      
        1 attctgttta gtgtttgtgg tttaggctgt taaaaaatgg tttgtttttt aatgtgcctg
       61 agttaattta taatttgcta atggaaaatc taaaaaaata cttttacctt gatccttcag
      121 ttacatttct caatcatggc tcttttgggg ccaccccaaa acctgttttt gaggattatc
      181 aaaattggca attacgactt gaacaacagc ctgttctttt ccttggaaga gaatatgacc
      241 agttgttgtt tgatgcgcgg gcgaaattag gtgtctactt aaacgctgat ccacaggatt
      301 taatttacgt gcctaatgca acgcatgggg taaatgttgt tgcccgctcc ctgcatcttg
      361 aacccggaga cgaaatcctc acatctgacc atgagtatgg tgcctgtgac tatacctggg
      421 actttgtttg tagaaaactt ggggccaaat acattcatca acccattcca ctgccagctt
      481 atactgagga cgagattgtc gatcagttgt ggcagggagt cactccgcgg acaaaggtca
      541 tttacttaag ccatattact tctccaacgg ctttacgcat gcctgtggaa aagatctccc
      601 gtcgggcaaa ggcggcgggt atattgacag ttattgatgc cgctcattcc cccgggcaaa
      661 ttcatgttga ccttcagaaa gttgatgcag attttgtgtt tggaaactgc cataaatgga
      721 tgttgggcgc aaagggatcg gcatttttat atgttagaag ggagctgcag catctgattg
      781 atcctttgat cgtgagctgg ggatacaacg cgactccaga aacaaccaca ggatctcgtt
      841 ttattgatat tctgcaatgg acaggtacca aagacccagc cgcggctttg accgttccta
      901 gcgccattca atttatggag gaaaacaatt gggatgaggt gcgaaaagag agccatgatt
      961 tgttgcggca aactcttgag caaatttgtg aaatggtgaa aatggagtct ctatatccgc
     1021 tcgactctga tttttataac caaatgggca ttgctccgct tccgcaatcc aaccttgcca
     1081 cacttaagag ccgtttgtat gatgaataca aggtggaagt gccattgata caatggcaaa
     1141 acaggcagtt tgttcgtatt tctgtacagg ggtacaattc tcaggaagat ttgaataaac
     1201 tggtcaaggc tttacagatt ttgttgcctc aagtggcggt ttagttttgc atgtaaaaca
     1261 atacatgcat tgacttatac cgcccatgat agtggagatt ctaaacttga aaaaatcaag
     1321 atcgcgaaac ctatctcgtt ggttttcctt tcttttgttt tccgtgctgg tttgtgctgt
     1381 gctgttgcca accccgggta aggcggagtc tttgcctcgc cagaccatta acacaccatc
     1441 tcagttgata gatgctgtaa acaatctgcg tatagccaat ggattgcccg cgctggctgt
     1501 ccattcggtg ttaatgcagt cttcccaatc tcaggcagat tatatggctt cagttgggta
     1561 tgccacccat gagcgtccag gtgggattag ctttacccaa caattgcttt ctttggggtt
     1621 cccgcttgcc ggtgacctgt cactcggcgg ttttcgtgct gagaatgtga tccaatcttc
     1681 caatccgttg gtatggaatg gtgtgccgcc tgcctggcag gatagtcaac acatgaacac
     1741 catgctttcg cagaatttta ctcatattgg agcaggaatc tcgcagggcc caagtgggta
     1801 tttttatgcg ctagattgtg cagctgccac cggtagtggg gaaatgcagg atggtgtcac
     1861 tacattgcta accagtgtgc ctggtggggc acaagttgga gtcagccaat atatggttcc
     1921 tgtgatgttg agtacggcaa atgccaatgg agatgtgttt cataaagtgc aatacgggca
     1981 aactttgtgg agcattgcca ttgaatatgg aacgacgatc aaagcgattc aggcattaaa
     2041 cggcctcgga gaagacttgg tggtatatca gggacaggaa ctattggtcc taaaaggagt
     2101 gactccgccc ccagcagcta caccgaccat ggttcaagtt atggaaactc ccacttctgc
     2161 ggaattgata acaactgaaa tgcccattcc acctgtcaca ccaacatcaa ttcctcaggt
     2221 ggtaaccgta cccgcaacag atatgcctgt ggaggaagaa gggtcatcga gtaattcttc
     2281 tggtttgctg gtggggatct tggttgtggc tgcctttgtc ggtggtgggg tggcggtctg
     2341 gttgattcgc gacccgaatt aaagtgggca atgatccatg ttttgtgcag gctgtgcttc
     2401 gagtgcggcc tgtttgtttt tgtaagatcc cagtaatagg tatcaaatga ctgtaaactg
     2461 gcaaaaggta taattgctct tcgcaatgca gtctaataga ctgccaaaat acaaaactac
     2521 gaaaagaaaa ctatggaaat aacactggca ttaggcggtg gcggggccaa aggcaattca
     2581 catcttggcg tattgcgccg tcttcttaaa gaaggatttt tgatccgctc tgtggcaggg
     2641 accagttttg gtggtctcgt cgcagttttg tacgccattg gatactcacc agatggcatt
     2701 caggaaattt ttgaaacagt agatcaaagc cgtttgtatg gacgccaccc tgaggatggg
     2761 ccatctttgc ttggcctggc aggtgttcgg aaattattgg acgaggtggt tggtcagcgt
     2821 accttcaaag atctgcgcat tccatgtgcg gttactgcgg tggatgtcaa atctgggtgt
     2881 gaagttacga tttctgaggg gccgttgaaa gatgcgatcc ttgccacgat tgccctgcct
     2941 ggtatttttc ccgcacaacg cataaacgat tgggaattgg tggatggcgg tgttttgaat
     3001 cctgtgcctg tttcagtagc gcggatgctt tcaccgaaac tgcctgtggt ggcagtgatg
     3061 ctcaacgatc ccatggataa acctgtacct gcctacacca ttcccatgcc gcagatcctc
     3121 cctcgctcca tcgccgaacg gatcacacgc atgcatttcg cccagtcgct cgatatcttt
     3181 ctgcgtgctg tggatgtaag cagccgtgcg gtggcgcatt ttcgtcttga gttggataag
     3241 cccgatgtgc ttatccgccc gaatgtacat gatatcaacc tgctggataa agtggtggta
     3301 tccgatgtca tcaaacttgg tgaacgggcc actgaagagg ttctgccaca attgaagcgt
     3361 gcggtttcat ggccagcaag agtaaacaga agaatttttg gagcgataca atgagtagag
     3421 atttaaagga atatgaaaag caaaccaacc gccgccttat tattggcgca atggttttat
     3481 tgcttgtcgt gggggttggc ctgatatggt tgatctatgg cggtggcgca gcttcaatgg
     3541 gatttttctg cctgatggtc gggctggttc ccgtggtgct gatcgtcgga atcttttacg
     3601 cggcagattg gatcatgaag aatgccggac gcaaataatt cgaccttgat cacgtttaac
     3661 gactggacct tgcgagtccg cgaatcgaat tctgattctc cgcggctgct tgtgatgctg
     3721 catggtttta ctggcgatga aaattcaatg tgggtatttg cccgcaggat ttcgacagac
     3781 taccatttga tggcgccacg cgctccacac cccaccgaac caagcggatt ctcctggcgc
     3841 ccgtctcaag gcgggacctt tggcaggccc agcctcgaaa tgctgcaacc agcggctgag
     3901 gcgctgattc acctcattga tgagtatgca gattcggtta agttggatgc gtcgcaattc
     3961 gatttgatgg gattcagtca gggggcggca atgaccaatg tggtcggctt gctctatccg
     4021 cagcgtgtcc gcaagatggg tgtgttggca ggattcatgc cttcagggtt ggacgaacag
     4081 atcgcacaaa aagtgctggc aggcagaaat atttttgtgg cacacggcac gcaggatcaa
     4141 atgatccccg ttgaccgtgc ccgtgcttca attgaattgc ttgaacaggc aggcgcgcag
     4201 gtgacctatt gtgaagatga agtggggcat aagttaagcg caaattgcct ttctgcgttg
     4261 gaaaattact tgaataatta aggatttctc acaaggctgt tgacgctgat tttgtttgca
     4321 ggtatccttg ctgaatgcga accaaagtcc gcgattcatt tttatacaaa ggtaaagtat
     4381 gaagcaaaat ttttcccgtc gtgattttct aaaagttgca ggtgttgggt tgggtgctct
     4441 ggcattcaag ccatactttt ctgaaaccta ttacgaaaaa ctttataccc cgaaacgcct
     4501 gcctcaattc cctgcgggcg acattatcgg ccgccttgtg gatcagaccg acgtacgcag
     4561 ccgcccggat aacaacccga tcttgaacaa tgtgatcgga gccatgcccg ccgattattt
     4621 gatgccgtgg ggacgtgagg tgattggcag tgtgatcggc ctgaccaacc aaaagtatct
     4681 tgagaccccg cagggatatg tgtgggcctc acgcgtgcaa ccgacgagga atttaccaaa
     4741 taccccgatc actgaaatgc cagcgggtca gcctggcttt tgggcggaag taaccgtccc
     4801 atatgttgac ttgaccctcg aaacagcccc catctcgccg tggatccaaa gcctgatcca
     4861 atacaacttt ccgccgcgtg tctattatgg gcaggtgttg tggatcgatc aggttcgcac
     4921 tacgaatggc tttgcggaat atcggtggaa tgaatcgcct gggcatgggt atgggtttgg
     4981 cgatatttac tggctggatg gcgcgggttt gaaagtgctc acagatgtgg atgtggcgcc
     5041 gatcagcccg gagatcgacc ccaatgagaa aaagatcctt gcagatctca cctaccagac
     5101 cctttcctgt tatgaaggca ataacgaggt tttcttctgc cgcatttcca gcggtgcaaa
     5161 attcgatgcc acaggcgcgc tttctgaaga acgggcgacc ccagtgggtg agttgttgac
     5221 ccattggaaa atcatttcac tgaatatgag cggcggcggt tcggcttcgg ggtattccac
     5281 acccgccgtg ccgtggagca ctgtaattca tggcgatgga attgccattc acggtgcgtt
     5341 ctggcacaac gattttggtg aaaggcgttc gcatggatgt atcaacgtga cacccgaaga
     5401 tgccaagtgg atcttccgtt ggacaacgcc ttacattacg ctggcacaat gtgaaacccg
     5461 catgacctgg cccgatcacg gcaccgtggt caccaccaag gaattgaagt tgtaatcaag
     5521 atccttcgtc ctgagcgaag ggcgaaggat ttaatttatg aggtaatcaa atggaatttt
     5581 ctcaaatcag tgttgggctt gcctttcttg ccgggctggc ttccttcctt tctccgtgtg
     5641 tgttttcgct ggtccctgct tacgtagggt atctgggcgg gcgtgccgca ggtggtgaaa
     5701 gcaatagcaa aaaccgctgg atcacattca gtcacgggct ggcttttgtg ttgggattta
     5761 gttttgtctt cgtttttctt ggtgtggctg cggcttttgc aggtggggtg ctgtttgacc
     5821 tgcgtttctg gctgaccaag atcggcggcg ttgttgtgat cgcctttggc ctgcacatga
     5881 tcggcgtttt tcatattccc ttcctggcgt atgacacccg tgttcaacag gcaccagacc
     5941 cgaagatggg ttatctttcc tcggcgctga tgggtgtatt tttctcggcg ggttggtctc
     6001 catgtgtggg gccagtgttg ggagccattc tcaccgtggt tttcaatggc ggctcgattt
     6061 cacaaggcgc agttttgctg acctcttatt ccatcggcct cggaattcca ttcctgttcg
     6121 cggcattggg cattggctgg gtaaccaaca ttttgaggaa ctacgccaag accatgcgtt
     6181 atgtggaaat tgtcatgggt gtgattttgc ttgtggtcgg cgtcatgctt ttgacaggtg
     6241 tgtttgaaac ccttgcgttg cagggtcaag gcttctatat tgattttggt ttgtaattcc
     6301 atcccccttt gacagatata aagcatcatc aaacccgcgc ggatccttca catccgtgcg
     6361 ggtttgatga tgaaacaagc gataatgaga ggcctttccc atgcttaatg taaccctcta
     6421 cacccgcaag aattgcaaat tgtgcgacga agtaaaacac gaattgcagg aactgcaatc
     6481 aaaatatcca caccgccttg tggaagtgga tatcgaatct gataccgcgt tgatcgataa
     6541 atacggcatc gaaattcccg tggtggaagt tggtccttac acattgaagg ctcccatttc
     6601 acgacagaat ttacaaatga ccctcggcgc cgctgcagac aggaaaaacc aattggaaca
     6661 ggtcggcgac cctgattatc aaatccgcgt caagaaagga aagaccgtga ccactggaga
     6721 ccgtgtttcg ttttggattg ccaaacgtta tctgttgctt ttgaatctct ttatgttgtt
     6781 ttatgttggc ctgccgctcc ttgcaccaac attgatgaag ttgggcgcag aactcccagc
     6841 cagggtcatt tatcgaatct acagcccgct ctgccatcaa tttgggttca ggtctttctt
     6901 cctgtatggt gagcaggcgt tttatccgct ggcagaggcg aatgtggcgg gggtaaagac
     6961 tttcgaagaa gtgacaggca ttccaaatct cgacgatccc tacagcttca cccgttttga
     7021 agcgcgtgcc tatatcggcg atgagacggt tggctataaa gtggccttgt gtgaacgcga
     7081 tgtcgccatt tatcttgcca tccttctttt cggaatcacc tatggtctta cgggccgccg
     7141 catcaaatca ctgcattggg tgttgtggtt gctcatcggc attgcgccca ttggctttga
     7201 cggcttctcg cagcttttca gccaattcaa ctggacctgg cttgccactg tcgttcccta
     7261 ccgcgaaagc accccattcc tgcgcgcact cacgggcgcc ctctttggcc ttgccactgc
     7321 atggtttgcc taccccaaca ttgaagaatc catggcagaa acccgccaat attacatcaa
     7381 gaaattcgcg gtcaacgagg cttcgcaata atgccctttc aagaacacga tggtcttcgg
     7441 tattaccgtt ttgatatttt ttcaaacgct gtaacacagg ctgtctttac ccgccgtggc
     7501 ggggtcagcc ctgcgccgtg gcattcgctt aaccttgggg gatccgtagg tgacgaccct
     7561 gcgcacgtcg ccgcgaaccg catccgtgtt tttcaagcca tgggatgtga acccgaatcc
     7621 atccacgacg tgtggctggt ccacggaaca gacattgtct acgcggatgc acctcgtccg
     7681 cttgacgagc cttccgcgaa ggccgatatc attttcaccg acaatcccag ggtttcattg
     7741 ttcatgcgct ttggcgattg tgttcccatc cttttccacg acccgaagaa aaaagtcatc
     7801 ggcattgccc atgcagggtg gatgggaacc ctgcgcggtg tggtgggtgc cgctgtggat
     7861 gggatgcgct ctcactatgg cagtgatccc aaggacattg tggctggcat tggcccttcc
     7921 atcggcgtgg accattacga ggttggcgct gaaattctcg cgcagtttcg tgagaaattc
     7981 aacagccatg ctgatgccat gttcgaattc agggaaggcc gtatttacct cgatctgtgg
     8041 aagtcgaact cgatgcagtt gcagcaggca ggcgtggagc agatccaggt ctcagaggtt
     8101 tgcaccgcct gtcatttgga tgactggttt tcgcatcggg cagagaaagg taaaacgggt
     8161 agattcggcg cattaatggc gttgcaggcc taacgttagg aaagatgcgg ggtaaaatta
     8221 aggcatgagc gatctggtca aatccatccg agaaagatat ctggcaacgc tggataaaat
     8281 tgcatccgcc gcccgcagtg ctggccgaga ccctgattcg gtccgcctgg tggtggtgac
     8341 gaaggcccag cctgtggaag tggtgcgcgc tgtgatcgaa gcgggcgcgc aggtgctggg
     8401 agagaattac cccgaagaaa gtgtaaagaa aatacaatat ttgggtcagt tttctgcggt
     8461 agaatggcat atgatcggtc acgtgcaaag ccgaaaggct caactcgtgg ctgcgaactt
     8521 taactaccta cattcgcttg atagcctgaa attagccaac cgacttgacc gtttttgcag
     8581 cgaggcgggg cgtgtgctgc ctgtgttact ggagtttaac gtgggcggcg aagagagtaa
     8641 atcgggctgg tctgcatggg acgaagcgca atggcctgcc ctgcgggatg aacttggtgt
     8701 ggtgatgtct ctgcccaact tgaaagtaca tggactcatg accatgccgc cgcttggcga
     8761 aactgccgaa ttctcccgtc cgttttttca aaaattgaag cggctgcagg cttacctttc
     8821 cacgcaactt cccaaatccg atttttcgga gctttcaatg gggaccagtt ccgattacga
     8881 agttgccgta caggaaggcg ctaccctggt gagagtcggc actgccatcg ttggtccgcg
     8941 acaatataag acggaggttt gatggatttt cagattttga ttttgttgat aaggaatctt
     9001 tccactcttt tcatttgggt cgtgatcgct tattcattgc tttctttttt tgttcccccg
     9061 tatcaccctg tgcgtgaggc gcttgagcgc attgtaagcc cgtttcttga cccgatccgc
     9121 cgtattctgc ctgctgctgg cccgatggat ttcagccctg tggttttgat tcttgccatt
     9181 gattttttgt ctcgtatcct gattgccttc ctaatttctt tgagttgagg tgaaccatga
     9241 ctagaaaatt cgttttacat agcggtcaac gtgggtctgc tttggcagtg cggattacac
     9301 cgcgtgccag ccgcaaccag attgtgggtg tgttgagcga tggcactgtg aaggtacacc
     9361 ttgcctctga acccgctgac gatgttttga atgtggaatt ggttgggtat ctttccgagg
     9421 tgttgggagt gcccaagacg cgcgttgaag tcgttgctgg cgaaaatggc cgcgacaaat
     9481 tggtctcgat tttggatatg gacgttgaga ccgcgcatca acgcattctt tcacacatgg
     9541 aataaacctt tttgtttttt cttcgcggcc atgcaaatga caacttgaac cgcgaagttc
     9601 gcgaagaccg cccttcgcgg taaaaaattt ttgaaatata nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn atataaagac gcgaagccgc cagaattctc tgcggtcttc
     9781 gcgtctttga gttaatgcct tattgcctta ttctgaaagc ccgtacaagc gccgatagat
     9841 ggtgtaaatc tcgtaaatat tgcggatgta attgcgcgtt tcctcaaagc ggacggattc
     9901 aaggaacaag tcggggtcac cctgcccaag gtcacgccat tcgatcgcgt tgccagggcc
     9961 gccgttgtat gctgccagtg ctgcatacag gtcgccaccc agcaggtcgc gattgtttcc
    10021 gaggtagtgc gttccaaatg cgatgctcac atcggggcgg tacaggtcgt catctgtgta
    10081 gttgacaggc cagccaagct gcgaaacgat ctgcgcacca gtggagggaa tgacctgcat
    10141 caatccgcgc gcacctgcgc tggatttgac aaacccctcg aacaggcttt cctgccgaat
    10201 cacgctaaaa acaaacagcg ggtcaaagcg gtttgaatct gcatcgggga gtaccaggtc
    10261 tgaatagtac aacccatagc gcacatggct gaaataggga ggagccatca ttgattcggt
    10321 gtgttcatcc agccctgcga tggtaagcac ctgccgtgcg gcaaagatcg ccgagcgata
    10381 caaaccaata tccaccagat agttcatgag ccgataactt cccacggcgt ccatgtttgt
    10441 ttccagttcg gcgcgcaggt tttcgaattc aaggcgggcg tcttcgtaca ttccaagttc
    10501 catgaattcg gtgccgcgaa tggcccgtgg atcgctggcc aatgacccca atccgctcag
    10561 gtctgtttca agcggaagat tgaaagtgag ccgcacccaa gaatcggcat ccttgcgttc
    10621 agattcgaga ttgggtgtca ggttgaccga aatcggggct tgaaagggtt gtctttcgag
    10681 caaaaggtcg cgggcgcgtt cgctgtaata accgccaggg tcacggtctt gcgctgctcg
    10741 ccacgcattg accgctgctt cgccatcacc aagtttttgt tgggccttgc caagccacag
    10801 atacgcacgt gattggtcca ccgccagcac agagagattg aggctgcgat tgaacgcatc
    10861 caacgcaggg acggtgttcc cattgcggta atggatgatc cccgtcagga aagtggccgt
    10921 ggaagcctgc tctgaacctg ggtattcatt tgcaacccgc gcccagattt gaatggcttc
    10981 ttcgtacttg ccgttgcgct cgtaaatgcg cgctgcgctc atcaaataat ctgctgaagt
    11041 ttcgctgcca gggacagccg caacaaaatc caaaagagtt ttgatgccca cagtataaga
    11101 gccaaggtta taccactcaa taaaagcctt ttcgccccac gcatccaccc agcgcggatg
    11161 cgtgggatac ccttcgatga atttcttgta atcctgcagg gcggcatcgt aattgccaag
    11221 gtcgcggtgc gacaaggcgc ggtaataata ggctgtccca tcgttggttg gattctcaac
    11281 aaggtactga ttgaaggccg ccacagccac atcatattgc cctgcaaaat aatccaccaa
    11341 accgcggtca agctgactga ccgtgccgcc tgcctccaac agcgcaacca gactcagata
    11401 cgaataatac gaaagcggat attgctccac tgccagacga aaacgtccca gcgcctcttc
    11461 gttctgaccg atggcctgat acgccaaccc tgactcgtac gccgtttgag ctttgatgta
    11521 atcattggat gcgcgtgcga aaactccatc atacaaggcc agcgcggagt tgtaatcgcc
    11581 gatggcaacc tgggttgaag caacttttag atcgagaaga gtgtcatcgc caagtgcaga
    11641 ggcttgaatg gcggcagtgt aagccgaaag cgcactgggg aaatcccgtg cttcaaacag
    11701 ggcatcgccg cgcaattcct gcacatgtgc atccaggtga ccaggccgca caacgagata
    11761 ggtctgccac gaatctgcag ccgcttgata atttcccaaa cggtaatttg ccaatccctg
    11821 cagaaaataa gcgtgaccga ggttgggaga ttcgggaaat tgtgagatca attcctgtgt
    11881 ggaaagcagg gtttcttcgt agcgctcttc tgcaaaacgc acacgcgcct ccccccattt
    11941 tgccgccgcc tgcaccagcg gatcgggcga atcgctcaac gcagtttgat aatggagcat
    12001 cgcgctttcg taatctccat tgaacagggc gtgatcgccc ttgtccacac gggcgatggg
    12061 cagcggagtg ggggtgatgg tgggggtgga ggtcgcttct ggtgtgggag tggtgcttgg
    12121 ggtcagggtc cagcctgggg gcagggtggg aaaattcgga aagatggaac atgccgttat
    12181 cgccgtggca agccaaagtc caagaaagaa gcggagacct gtgcgcaggc ggctgcgtcg
    12241 cgtgtcagag tcgattcgtc ttgtggatgg tcgggtgaaa ttcattccgt ttttatactg
    12301 gtaaaagaca ggtgcgtcaa gcggggtttc ggggtttgtg tgtgatatat taaccgctgc
    12361 catgatttgt ggcacatcaa aataaaaaga ggaaaaaaag aaaagaaaat gaacatacac
    12421 cacaaaattt atcattttgg acggttgatt ttgtctgcgt ttatgttgtt gagcattgcc
    12481 gcatgtggag ccaggccgca ggaatccacc gaacacgtaa cctccagtgg atttgtctgc
    12541 cccgaaccca gcccgcgcgt ggaatttgaa agcaaagaga tcaatatttt tacatggaca
    12601 gaatatgtgc ctgccgacat gatcgattgc ttcggcctgg tctacggcgt ggatgtgaat
    12661 gtggattatt tttcctcgaa cgaagaactt tatacgaaga tgtcttttgg cgaaagcgtg
    12721 aatccctatg atgtggtcca ccccagcgat tacatgatcg atgtgctgat ccgtgaaggt
    12781 ttgctgcaga aacttgatgc aggcaaactt gcaaatacga aaaacctgga tggcaatttg
    12841 atcagcgcgt atggtaattc gcttgatttc cttgtgccgt atcaaatggg aacacaagcc
    12901 atcgtgtaca acagcgaaac cgtcaagacc ccgccgacct cctgggcaga tttgtggagc
    12961 cctgagtacg aaggccgcat cgttgccgtg gatgataacc gtgtggtgat cggcatggcc
    13021 ctgcttacat tggggtacga tgtcaattcc accgacgaag cccaacttga agaagcaaaa
    13081 caaaaactta tcgaactcat gccgaacatc cgcacttttg atagcgacag ccccaaaact
    13141 ccgcttgttg ctggcgatgt ggaccttggc atcgtttgga atggcgaagc ctttttggca
    13201 aggcaggaaa atgccgcttt tgaatatgcc tttcccaagg aaggttcgat catcttcttc
    13261 gatggtatgg gcattcccac caatgccccg caccccgata tcagctacgc ctggtttaat
    13321 tacatgatgc agggcgatgt cttttggctg acactggtgg attatcccta caccattccc
    13381 aatcaagccg cgctcgattt cgcaaaagcg aatcatgcag aggtgtacga agcctacatg
    13441 gcttcaccca ttaccaatac ccccgctgaa attttcgcgc agggacataa agtggaagac
    13501 cttggcaatg cgctttcact gtacgacaag atctggacgg aaatcaagga gtagcctgaa
    13561 atcatgctga atttttctcc cactgccagc gaggcggaat tacgcctcgc catcattgaa
    13621 tgcgggcgca ttgcctatga gcggcatctg gtcacttcca acgatgggaa catttccgcg
    13681 cgcatgctgg acgggaatat tctcatcaca ccttcgggta tttcaaaagg ccgcttgaac
    13741 cccgacgata tgcttgtggt tgatctggaa ggcaaagtta tttcctcccg ccccgaccgc
    13801 aaaccttctt ctgaaacgcc catgcacctt gaggtgtaca aacagcggcc cgatgtgcgc
    13861 ggtgtgctgc atgcgcatcc catctatgcg accacgctta ccgtgtcagg tcttcaattc
    13921 cctgtggatg tattgcccga agtcctgctc acacttggcg atgtgcccat tacggcctat
    13981 gccaccccct cttcacacga agatgccgag gtcattcgtc cttttgtcaa aactcacaat
    14041 gccatgctgc tttgtcaaca tggcagcctc acctacggca aacatctcga tgaggctctg
    14101 attcatctcg aacgcattga acacgtctct gaaatttact ggcgcgcaaa aatgctgggc
    14161 gaagtgaaac gtgttccgcc cgaggcacag gcgaaattga tcgaattgca cgaaaaatat
    14221 ttttcatagc atcgtttcaa gcgataaagg ataaaccatg cgtacagttt tttgggaaaa
    14281 caacgaattg aaaatgatcg accagcgcat tctgcctgcg cgttttgaaa ttctctccta
    14341 tcgcgggcac aaggaagtgg cgcatgccat caccgatatg gtggtgcgtg gcgcgcctgc
    14401 gattggagcc gccgccgcct tcggcctggc tcttgctgga tatgaatccg catccacctc
    14461 aaccgaaggc ctgctcgctg acctacgggc atctgccgcc gttttgaagg cttcccgtcc
    14521 aaccgcggtc aaccttgcgt gggccgtaga ccgtttgatg cgtgtggcgg aacaaatgaa
    14581 agacgaagga aacgcagacg ccgtgcgtgc ggctgttttg accgaagcgc aaaacctcgc
    14641 cgatgaagat gtggaaatca acaagcgcat ggcggagcac ggcgcagccc tcatcaacga
    14701 tggcgacacc atcatccatc actgcaacac gggtgcgctc gccactgtag attggggcac
    14761 cgcattgggc gtgatccgca ccgcgcacga acagggaaaa cggattcacg tcctcgttga
    14821 tgaaacccgt ccgcgcttgc agggtgcccg ccttaccgcc tgggaattgg aacaatacgg
    14881 cattccctac gagatcatca gcgataacat gtcggggtat tttctcaagg ctggcaaggc
    14941 gcaaaaagta ttttttggcg ctgaccgcgt ggccgccaat ggtgatgtag ccaacaagat
    15001 cggcacctac atgctctctc tggctgcaaa cgataacaac gtccctgctt atgcggtcgt
    15061 gcccacctcc accgttgacc tgtctctcgc acacggcggc ttgattccca tcgaagaacg
    15121 caataccgct gaggttcttg gcattcaatt tcaaggtgaa cctgttgcac cgaaaaatgc
    15181 caaagcccgc aaccccgctt tcgatgtcac accgaatcgc ctgctcagcg gcatcgtcac
    15241 tgaaaatggt gtagtgtatc cgccgtttga tatgaatctc aaaaaggttg tgaaataaat
    15301 gctctttcca tattcccctc ccacccgttt tgacctgaga ttttccatcg ctgggatccc
    15361 gattcgtgtc cagcctttgt tttggctgat cgcggttctg ctggggtcca cctctggcgg
    15421 gatcatttcc cttgtgatct gggtgctggt cgtcttcatt tcgatcatcg tccatgaatt
    15481 ggggcatgct tttgccctgc gccgccatgg catctcttcg aacattctcc tgcatgcttc
    15541 aggcgggttg accgtacccg agtcaatgcg gtggggaggg gggatcgcca gtgtggcact
    15601 cactccaaat caggaaattt ttgtttcgtt ggcaggtccg tttgcagggt ttgggttggc
    15661 gctgctggtc tctgttttga cctttatcat gggtggctcc atcgtaatgg gtacgatcct
    15721 ttttgtgatt ccagttcctg tgcatgcctc cctgccgttc aatttgtacc ttttagacct
    15781 gttcgttaca gacctgcttt gggtcaatat cttttggggt gtgttcaacc ttgtgcctgt
    15841 ctatccgctt gatggcgggc aagtgaccag atacattctg ctcatcgctg atccctggga
    15901 cggcttgcgt aagtcattgt ggctttcgtt catcgcaggg attgtcattg ctgccattgc
    15961 tttctttttg ctgggcagca cttacatggc ggtattgttt gggtttctgg cgttccaaag
    16021 ttatcaagcc ttgcaaggcg gcttgaataa actgtaggtg aaataagtta ggtttgaagc
    16081 ccaagaggtc accctgcacg acagggtgac ctcttggaaa gtgattgaat cttgaggata
    16141 atttccgtat aataaaattg aaggtgggta tggggtggtt atgaattttg caaacgggga
    16201 tgggtcggga aaatatttaa cggttcaaat cttctttttg tgaacgatca attttataaa
    16261 ctgcctgacg ttgccgttcg ttttgattcc acttgatgaa aggagtgaaa aatgaaacgt
    16321 gtattttcca tgttggcagg attttgtctg cttgttttgc tgggtgggtg tggaccgtct
    16381 gcggaacaaa tatccacgat gacggcatcg gcgtggacgc cgacatcgcc tcctacgaat
    16441 accccggtgc cgaccaatac accactgccc acagaaacac ctgcgcccac agccaagcca
    16501 cccttcctgt tccgtgatga ttttgacggt caacttgctg atggttgggt gtggttgggg
    16561 gaagacacca cacactggag cttggccgaa gtgcctggct ttatgcgaat catcacccag
    16621 ggaacaaaca tcgggagtga tgccgaaccg atcaattttc ttgtccatgc cgcacctgct
    16681 ggggattttg aaattgaaac ctatgtgcaa ttcgagccga cgaccaattt tcagtttgca
    16741 ggcctgttga tctacgaagg ccaggggcaa gctcttggtt tcggacgggc attcgcccag
    16801 tgtaatttcc ctccctgcaa aggcaatgcg ttgtacttcg acaacccaac tcagcaaaac
    16861 ggcccgaatt ttgccacacc cataaccagc ccctcagagg tctatcttcg attgctccgt
    16921 gaaggcaata cctatacggc ctattacagt gaagatgcct caaattggac ccaaatcggc
    16981 cagcacacaa gcgatatcac acctgcgttt gtcgggctga tcgcgagcca ggcttatgaa
    17041 gaagagaagc ccgcagactt tgattacttc tccattcgtg ccattccgta atcgtgaaat
    17101 ggataagagc agcggcggtg tgatacacat ccttttcttt gacaatcaca ttatcccgtg
    17161 ataaactcac gtttgtaagt aaatccagcg cactcgcaag agtgcgcctt tttctgcgag
    17221 taaatcaatg gacattcttc tcactggttc tgttgcctac gattatttga tgaccttccc
    17281 cggtctgttc aaggaacaga ttctccccga acgcctcgcc tcgatcagtt tatcgttcct
    17341 cgttgaatcc atgtcgaagc agcgtggcgg cattgcgccg aacattgcat acaccatgac
    17401 gttgcttggt gaaaaaccgc gcgtgatggc aaccgtcggc gaggactttg gcgactaccg
    17461 tgcctggctc gactcaaaag gtgtggatac ggaactgatg aaagtggttc ccggtctgtt
    17521 caccgcctcg ttctttgcca cgaccgatca ggcttcggcg caaattgcat cgttctaccc
    17581 cggagcgatg gggcactccg ccacccaatc catcaaggaa ttgccgaaga aacccgatct
    17641 cgtcaccgtt tctcccagcg cacccgatgc aatgatgaaa ttccctgccg agtgccgcga
    17701 gctgggcatc ccttatttgt atgatcccag ccagcaggtg ttacgcctcg aagggcatga
    17761 acttgcccgt gatatggaag gcgcgcattt cctcttctgt aacgactatg aattcggtct
    17821 gatttcaaag aagacgggct ggagcctcga ccagattctc ggacacgtca aggtgctggt
    17881 cattacccgt ggcaaagatg gcgctgactt gtacgctggc ggtgaatcgg ttcacatccc
    17941 aactgtccct gaggatgaga tcgtggatcc cacaggcgta ggcgatgcct tccgcggagg
    18001 attcctcgca ggctacgcca acggtttcga ttggaagttg tgcggcgaga tcggttcgct
    18061 ttcggcagtg tactgccttg aacagcgcgg tacccagaat cacagttata ctaaacagga
    18121 atttatccag cgtttccgcg atcactttga tgattctgga aggttggatg cattattgaa
    18181 gtaattggta gttggtaatt ccgccaatct ccaattacaa gttgccaatt accaaaatta
    18241 gaaggagcaa tacatgagca attacgatat taaagatatt caacaggctg aaggtggacg
    18301 ccgccgtatc gaatgggcgg aacgcgaaat gcccgttctc cgttcgatcc gcgagcgctt
    18361 tgccaaggaa aaacccctca agggcatgcg tctctcctgc tgtttgcatg tgaccaccga
    18421 aaccgccaac ctgatgcgca ccctgcacga aggcggcgca gacatcgtcc tcaccgcttc
    18481 caacccgctc tccacacagg atgatgtggc cgctgcgctg gtgaaccagt acgaaatccc
    18541 cgtattcgcc atcaaaggcg aagacaaagt gacctacttc aaacatatcc gcgccgcgct
    18601 ggagcacaag ccccacatga cgcaggatga tggcgccgac ctcgtttcct ccctgttctt
    18661 tatcgaaatg ggacgtttcg agcgccttga accctccctc gctgaatggg ccaaaacctt
    18721 gagcgaggaa gagcgcaagc agatgttgaa gaacgtcatc ggcggcacag aagaaaccac
    18781 cacgggtgtc atccgtttga aggcgatgga aaaagacggc gtgttgaaat tccctgtgat
    18841 cgcagtgaac gacgcgctca ccaagcacat gttcgataac cgctacggta cgggtcaatc
    18901 caccattgac ggcatcatcc gcgccacaaa tgttttgctt gcgggcaaga ccttcgtcgt
    18961 ggctggctac ggctggtgtg gtcgtggtct cgcttcccgc gcacgcggta tgggcgctca
    19021 tgtcatcgtg accgaaatcg atccgatgaa ggcactcgaa gcagtgatgg atggcttcca
    19081 ggtgatgccg atgttcgatg ctgccaaagt tggcgacatc ttctgcaccg tcactggtga
    19141 cttgaacgtc atcgatggca agcacttcga agtgatgaaa gacggcgctt tggtggccaa
    19201 ctccggtcac ttcaatgttg aaatcaacat ccccgcactc gaaaagatga gtgttggcgc
    19261 tcccaacctt gtccgaccgt ttgtggatca atacctcacc aaagatggcc gcaagatcaa
    19321 catccttggt gaaggccgcc tcatcaacct cgcttctgcc gaaggccacc ctgcttccgt
    19381 gatggatatg tcctttgcca atcaggctct ctccgccgaa tacatggcaa agaatgccga
    19441 caagctcgag aagaaggtgt actccgtacc gaccgagatc gacaacgaga tcgcccgcct
    19501 caaacttgaa gcgatgggtg taaagatcga catcctcagc gatgaacaat tgcactacct
    19561 caactcctgg gaagaaggca cctagatcac agggaaagtt tgaagtagaa atgaaagatg
    19621 aaaacagcct cgtgatattt cgcgaggctg ttttttgaaa tgagatcatt tcccatgcat
    19681 gccagaataa atcatattca ctttgtggaa gattcacaaa aaagcccagt tgtctttgtg
    19741 catgggtttc ccgattcgcc tgccatgttc gcggcgtatc acaccgaggc cgagcgccga
    19801 caagcctggc tgcagggtcg cagtatttac gcctatgcat ttccaaaccg tcacgacaac
    19861 ccgaattttc catcgctggg tgaattggct ggcggtgtga tggaccgtga attcaatgca
    19921 ttgatggatg aacttgcccg aaacagcccg acggggaagc tggtcattgt tgcccatgat
    19981 tggggcgcca cccatacatg gcgctgggcc cgcgagaaga agcatccgcc cattgaaaaa
    20041 ttgatcgcgc tctcggtcgg ctcctctttt cgttatgatg tttttgaaca tgggctcaat
    20101 gcgtttacat ggctttacgg actgtggttc tgtgcggcgt ggtatctgcc gtttttacgc
    20161 aaaaccgttg ccgcttccat cgtttcacgg gcggggtatc attctgaaac cgcgcacgaa
    20221 ctctggaagg atgcctatca ttattgggat cgcccaagcc tgctgatcac catttttctg
    20281 caagccttgt tctttctttt ttacaagcca gagtatcttg attttgattt ccccatcctg
    20341 tacatgcgga caaaactgga ccgcatcgcc tccacggcga ggtttgagca ggcgttacgg
    20401 acacgaccgg atgcgcgatt tgtcctcttc gaacaattca accactggtt tcccgaacag
    20461 cacagcgacc ttgtccttgc ggagatccgc tcttttgtgg attgatctcg ggaacagtgt
    20521 gtttttctca acaaggtaca atcaggcagg agaatctcat ggataaaaag atcaaaccct
    20581 ccgaggtcat tgccgcacgt tttcaggtct caaatgagac tgccaagttc tttctggggc
    20641 gcgttcagaa gagtttcaaa actgagagac cccctcatca actggtcgtg gattttatga
    20701 gcgggcaaag ctatgatgcc ctgcccaagc cgtaccagat cgcaaaagcc ttgaacgaaa
    20761 aaggcatctg ggcctatcca ttgaattcag aacccgccga acccgtggac gaggatgaat
    20821 tgtattaacc acataacctt ctccattctt caatggaaag ggtctctgcg cggcgcatga
    20881 aatcgatccc tgccgcggtg agcatggact cggcttcatg cggcggaatg tgcaggccag
    20941 aggatagaga attgcggagg gttttccgct tttgagaaaa acccgccttg atcagtttga
    21001 agaaggtatt taacaattca gcagggatga gcggttcctt gtgaatgtca attcgcagaa
    21061 tggcagaatc cactttgggg acggggtgaa atgccccagc aggaattttt gcggcaatgc
    21121 tgggcctgcc gtacacctgc acacttaatg ccagcaaact caggtcgccg ggtgcagcac
    21181 agatgcgctc ggcgacttcc ttttgaatgg tcagcaccac gcggcgtggt ttgggggtgc
    21241 tttcaagcag gtggcggatg atggctgagg taatgttgta ggggatgttg gcagcgacga
    21301 gataatcggg ttgttcgatg atctctgaga tgttcagctt gagaatatcg ccatggatca
    21361 cgttgacatt ttgatacggg gagagaactg cccgtagcgg ggcaagcagg tcagggtcca
    21421 gttcgaccgc tgtcactttt tgggcagagg ctgccaggta acgggtcaaa ctgccaaggc
    21481 caggaccaat ttcaaggaca cagtcatcgc cctgaatttc agcggcgcgg gcgatctttt
    21541 caagagcgga ggaatcctgc agaaagtttt gcccgaggct tttatcggcg cgcagttgaa
    21601 agcgtttgag aacggcgtgg gcatccagcg gcggaatgga acggtaatcg gtcattcgca
    21661 gatgatctct tcttcgtctt cttcatcggt ggtgatggtg ttgggttcgg gccatgtgcc
    21721 tgcctgaaac tttgcggtgt agtcgcgcag gatgttgaaa tccacatccc agtagaaaac
    21781 gttctttttg aaaacgggat cgtagatgcg gctttctttg aaaagctcct gcgggaaact
    21841 ggcaaagacg atgttttcag gcggcagttt tgtgccgagg caggcgagct gggcgatctg
    21901 ttcgggggtg aaatcggtga ggatgttgtc ttgaaaagaa tcgatcaatt ctggaatttt
    21961 ggtgacgacg gcagggctgg cgagcttttg acgcaacgcg cacaaaactc ggttttggtt
    22021 actggcccgt tcgaagacgc cttcttcacg gttgcgtgcc accttcagcg cctgatcgcc
    22081 attgaggtga tgtttgccga ttgtgaggcc tgtattttct gcggcagttt catcggggat
    22141 gttgatgtca atcccttcaa cggcgttgac cacgtttaca aaagtgcgca tgttgacggc
    22201 cacatagtga tcgacttgcg cgccgaagtt gaggttgatc gtgagagaga gcaagcccgg
    22261 gcctgcgctg ggatcatccc aataatgaaa gccatcgcca ggctggccat aaaggtaggc
    22321 ttgatttagt ttttcgtgat cctgcccatt gaggttatca gagatgtatg ggatttccac
    22381 ccacaaatca cgtggaaatt cgagcaccga aactttgggg gttacgaaat caatgcgtac
    22441 aaggcggatg acatccccaa gaccgtattg ataattatcg gagcgtgagt ctgcgccgat
    22501 ggctaccaca tgcataacgc tggtggttcc gccgcagcca gcgtatggag tgggggtggt
    22561 gaaggaaagg gttggggcca gcgtgatcat gttgggtgat gtggtggccg ccggggtcca
    22621 ggtgggtggc atggtctgtg caacgatcgg cagtgacggg ccaagcggct gggttacgaa
    22681 ggcgcggtaa cttctaaatg caaaaaaaac tgcagtgccc accaacacaa acgcaacaaa
    22741 cgaaagaatg gcgagtatcc ttttttggct ttgggtcatg ttctgttttc ttctctctgc
    22801 ctttaagtgg gaatttcaaa ttgccagtga gtaaaaaata acacgccgat cctccaagag
    22861 aagaatctgt gtcatttgct ggcacaaggt ttacttcaaa aaatacggca cctcggctgg
    22921 tgcgggtgca aggaaataca ccgtcaccca gcctgtcata tcctgaattg cgccatcgtc
    22981 aaatccgagg tcgatccatt ttgtgcgcgg cacaccgaat gcggtttcga tgatcggccc
    23041 gccgcctgta tcgccgatgg tggcggttcc ataaccgggg atgtaaacgc gcatccctgc
    23101 aagataactg tagatcgaat aatccactgc cacaatgccg taccctgcgc gcgcgccgct
    23161 ggcagtgctg taagaacatc cgcctgtgcc agattcgcag ggtgaataca ccgttgcgta
    23221 catttccact gccgtccaat attcgcctga aacaccgggc aaaggcgaca tgacgatctg
    23281 ttcaccgctt tttacgatgc gcggctgcgg ttcacgcgcc acgatctctt cttcaaggat
    23341 gcggctcact tcctgcccat tttcataccg aatccgtgtg cgcgtcattg caatgccatt
    23401 gacgccagat tgcaccaact cctcctgccc gagcggcaca tcaatggaac tgatcttttt
    23461 tgtttcgaac gggatcggct cgatcgccac atcaatgatt tcctggactc gcaccacctg
    23521 aatctgacca tcggcgggca gcgcttcacc acttaacggg aaactggtat ccaaaccgat
    23581 gagcggaatc cccgctttgg caagggcttc gcccaccgtc ccagccgccg agcggatgtg
    23641 taccacgctt tcgccgacct gcaccgtcaa atcgcgggct ggtgtaaagg tcacattcat
    23701 tgcggttgtg atgggagtct ctgcaggcgg gtcaagcaca tctgctgcgc cgatctggta
    23761 tccaacttcg gccagagcct gccccacatt cagcgcggag gtttggaagg tttccgttcc
    23821 ctgcggcgtg gtcaaagtca aatccactgc gcggcgtaac tgcaattgga cagaatcaac
    23881 ggatggcaat ggttggtcta tgggaaatgg aacaccgttc accagcacac ggtcaaattg
    23941 ctgcggggtg atcccgtttt gggtgagcag caccagcggt atccgtgaat cagatgcaac
    24001 gacctgaatc tgaccactgt caatgattgt gatggatggg gaagggtttg actggcaggc
    24061 gctcaacagc atcgtggccg caagcgccaa ccatcgtaga aatttcatgc agataattat
    24121 aaaccaccct taacttaatc cttgcagtgt tcatcctcgc aggtgcattc gagcccgttg
    24181 gccttctcaa aggcttcgcg tccttccttg tatgaaaagt agatcaaacc caaagcgccg
    24241 atactgtcta tgaaaccgaa cccgctcacg gcatagacca gactcgagat gagcagaatg
    24301 accgacatgt aaatgcaaac catcgtgcag ttcgcatccg ccagtatcgg catcgaatta
    24361 agcatccgac ctgttttgcg tttgcccatc accaaagccc acatcactgc aattgaaatg
    24421 accgaaatga cgatgccagg cagggtggta tctggcttgt gcccgatgac gaggttgtaa
    24481 atggcgctga cgccaagccc cactgccagc aggtaaaaag aggtgcctgt gatccgcagg
    24541 gcagtgactt caaaacgggt gcgcgatgta ttcgggtttt gccgaatgcg caataccatg
    24601 gcaaggatcc ctgccccgga aatcacctca atgaaagaat ccacaccgaa accgaaaagg
    24661 gtcagtgctt catcgctgac accgaatgaa atggaaacca gcccttctgc aatgttataa
    24721 aaaacagtaa atatggcaag ccataaggca tattgccagt acacggctgt attcgatttt
    24781 tgcaccagag tattcatttg gtctcctttt tgaaaataca ctttgcagtt tactcaatac
    24841 ggcatgaaca gggaagacaa atattcccat tgaaaattgt cgggaaagta tgtaaactga
    24901 cttcaacctg gataccaaag aggagagctt taatatgaaa aagatcgttg ttgtttttct
    24961 tgtgcttgtt tttctgctca cagcctgtgg cgggaacaag ccatccaaaa atttaagagt
    25021 ggatatgctg gaattcacct ttaccccggc tgaatttgtc gtcccggcag gggaggagat
    25081 caccatcaca gttgcaaaca atggcgcggt gctgcatgaa tttgtcatca tgaaatttgg
    25141 gcaaaccgtg ggcgatgatt ttggcgatga ggatgaaggc aatatttact gggaggtcga
    25201 ggttgagcct ggcaaaaccg tgaccaccac attcactgca cccgccgatt ctggcgaata
    25261 tcaggttgta tgtggcactg cgggtcattt tatggcaggg atggctggca cactggtcgt
    25321 tgccgccccg tagatgatca gcacttcaaa cagcggatgg ctcctgccat ccgctgtttg
    25381 gttttcggat acaatcggcg catggaaatg atcaccagcc ttgccaatcc gcttgttaaa
    25441 catgcccgag ctctgcgcca aaaaaagacc cgccttgaaa cgggtctctt ccttgtggaa
    25501 ggcattcatc atgtgggtga agccatcgaa gcggattgga agatcgaaac ggttttgtac
    25561 gcccctgacc tattgaccag ttcttttgcg cgcaacctgc ttacacgcct caagacgccg
    25621 ccccagcctg tttctgttgc cgtcatggaa tctctggcgg acaaggaaaa cccgcaaggc
    25681 atcattgcgt tggttcgctc ccgccaaacc cagctgaccg acctcaaaaa attttccagc
    25741 gctgttgccc tcgtctctcc gcaggaccct ggcaatgtcg gcaccattct gcggaccatg
    25801 gatgcggtcg gtgcagaaat tctcttcctg cttgaaggcg gcgtggaact gtatcatccc
    25861 accgtggtac gtgccagtat gggtaccttg ttttggaagc ccattgtcca agcctccttt
    25921 gatgatttca tcgcctgggc acgcaaaggc gggtatcaac tcatcggcac atctgccaag
    25981 gcagatgtgg atcatcacac ccttaccccg cacaccccat ggatcttaat gctcggcaat
    26041 gagcaaaaag gactcaccac agagcaatct gccgcttgtg atgtgaccgt ctccctgcct
    26101 atgaaaggcc gtgtcagttc gctcaacctt gctgtggctg cgggagtttt gttatatcag
    26161 tttggggagt agaaaagcgg gaagctggcg gggggcaagt cattttttct tgcttgtctt
    26221 ttttaggttt tcagcaaccc tgaatttgac aatttttccg accagtttca acggcagggg
    26281 ggcatccaat ggaaattgca cagacccctt tgcctgtttg tacatggata attccttttt
    26341 gaaagcctca attccgcttg gcgctggata aaagccaata tgtgttttga aagcggcaaa
    26401 atgaaccagg ttcccgttta ggaaaaaagt gggaatccca tagctgaatt tttcctccgc
    26461 gtttggagca gaggctttta tggctgcgcg taaatcattc aatagctttt gcgtttcttt
    26521 ggggaatgtc gcaatgtatt catcaatgga agcgaatttt gatttggtgg tttccatgaa
    26581 attattatac gcgctggcac atagctggtc aaatttctct gctctaattt ccgagtgcaa
    26641 cacagatcat tatttttctg atgttgattt tgattcggca tctgcttttg aggtgagatt
    26701 ttccacaagg tcgggcgcaa atagtttttt cgttttctcc tgttccagaa aaacgagggc
    26761 tgataaatcg atgacaagaa atacggccag aaaaacgatt gaaaagagac gttcatccac
    26821 agagttgaaa acgggcttat ctaaaaactg tacattgatc gaaactatac cgttgactgg
    26881 aagaataaag cctagcacca gcaaaccgcc aagcaggttc atgaacaaaa tccagaaagc
    26941 caattctctt ccagttgtgt tgagttttaa caagttccac cccgcggaca aaacgaaagc
    27001 gccgcttaag ataagagtta aattcattga cttgcccgct ccaaaaagag gtgaaaagaa
    27061 cctccaaatt tgtacaagcc ctgtgcatat catgattact gatattgaat aaattattgt
    27121 ttttcttgac attctgaatt tcctccagtg gttactttat ttgttttgtg aaatttccct
    27181 ttcaaataaa gtcaagccga ttatattgca caggcgcaag tcaggtcaac gcagccatgg
    27241 aaatctcctg tctgaaaaca aaaagcttcc tcgcgcagag aaagcttttt gttcaagttt
    27301 caattttcag ttaagcaaga agcggactaa aactcatctt cctcgtcatc gagccagtca
    27361 tcgtcttctt cttcttcggc gtcgtcgtcc cactcttcgg tttcatcttc ttcgtcccag
    27421 tcgtcatcct ttggggctac accatcggcg gcggaatagg tgttacaacc tgtatcggga
    27481 tcaaactcca ccgccgcggc agagcaatac ccttcgtcca cgaacacaca atctatgtag
    27541 tgacacttaa tacggggcat ttcgcttcct cctaagattg gggttgtctg atcaggcgcc
    27601 agacagctag aatgaaaatc agcgtcattg aaacctgcga agtaacacag aacgggcaga
    27661 tggctttcaa caatgcgatc tcaacataga tcagccagac cgtaaaaaga aaaccagtaa
    27721 gtgccatgcc aaagaccatc aatgtggcat tctctctaaa gaaccgatta cggttttcaa
    27781 aatacaacac agccaggatc gccagataac caccgatgcc gaacaccgca acgggaattc
    27841 cgtttacctc ggaatacctg ctggcattca ctgtatggca gtccccggag ccaaggcaca
    27901 tattgtcatt cccgctatat ttatagatgg tcatgtagat cgaaaccagc agccccagca
    27961 ccacaagcgc aacagatgcg cggtataacc atttatccat ttttcctcac aaaagtaatg
    28021 cttcaacttc ctggccaaca ggcacgcatt ttacaccagc gggaataata agtaaagcat
    28081 ccgcctgtac caacgaaagc aggttccccg aaccctggtg tcccgtcaaa aatgcggttt
    28141 ggattccatt ctcaagacgg atattggcgc gcagataact ttcacggcca tctgattcga
    28201 tgacttcccc gcatcgcact ttaactgtga gtctggtccc gtccaataaa ccgctcagct
    28261 tctcaagcgc gggccgcaca aaaacctcga accccacgaa cgccgaaaca gggttgccgg
    28321 gcagtccaat gaacggcagt cccttgtaag agccgaaagc cagcggcttg cccgggcgca
    28381 tgttcacgcg ccagaaatcg agtttgccgt ttgtttcgat cacatctttt acaaaatcaa
    28441 aagcgcctac gctgaccccg gcagacgaca cgatcaggtc tgcatgttca agagttgatt
    28501 ttttcaacag ggattccaca gattcgcgcg tatccttcgc caccccaagc cgaatcactt
    28561 ctgcccccac agactcaaaa gcggcagcca gtgcatacga atttgaatcg tggatcttcc
    28621 cttcgctcag cggagagtcc acttccaaca gttcgtcgcc tgaagaaagc agggctactc
    28681 tcggtttttt gtgaacatcc actttggcga tgccaagcat ggccagcaaa cccaggtcct
    28741 gtggtttgag tttgcgtccc ttttttagaa ccacgtcccc ggcatgcaaa tccatgccgc
    28801 gcggacggat attttcacct gctataacct tccgtgaaaa cgatacggtt tcgggtgggg
    28861 tggtgccagc atcgcgcaca tgaaagtcgg tgtcttccac ggggatgaca gcatcggcac
    28921 cggcgggcag ttgcgccccg gtcatgatgc gtgccgcctg gcctgtggcg agagtcacct
    28981 tcggcgctga gcctgccggg atatcagcaa ccacgctcaa ggttaagccc tgaacggcgg
    29041 cgttagccga gtctgcagag cggatggcga atccatccat ggatgagtta tcgaatgggg
    29101 ggaggtcatg cgccgcagtc acatccacgg ccagcacacg gccagaacac gccaccagcg
    29161 gaacactttc atgcggggtg ctttcgaagt gtgagaggat gcgctcgcgc gcttgaataa
    29221 ctgagtacat ggctaaaagc cacggcatta taacgcattc aaacttatgc catcattgcg
    29281 aagacgctgt atttccaccc gatgtggata gtgaccttgc aagtagaaat ccgctcgccg
    29341 ccatgaacaa acagagaatt aaaactgcat cccgccaaat cgtatttgga aatagccttg
    29401 aaagcatttc gccttcaaaa acctttatgg gaacaaaaat caccgccgca gcagaaatga
    29461 ccgcaactgc aaccgtgatc cacccgccgc gctgtacacc atgacgaaat tttgaaaccc
    29521 attccccagc ttgagccagc agggtgagga gaaagagaac tgcgagcgaa agagaccaga
    29581 tcgagaatgt cgttcgcaag gactgcttca cgctttcaat cgtggtgatc tcctgttcgg
    29641 taaaaacggg tgcgccgtta tcgagcctga gcctttgcag aaattgcggt cttctatcgc
    29701 tgcccatgta ttcaagcgtt tgcgaagcct gttgaaaacg ctctgacctg ctcaatccct
    29761 gcgcttcttg cgtgtacggc agagcatatt ccgcgcggat gaaggttggt gtgagcagca
    29821 gccgcgcccc gattccgaac aaggcaaggg gtacaagaaa cgcagccagc catgccagcg
    29881 aagatgaaac gagcggatgc gtttgtcgtt tttcgctgac ccgttgggtg tgcatggcaa
    29941 tggtcagcgc gatggagttc aacaaaaaac catacaccgt gaaagctgga tcagccccaa
    30001 acaggaagac cgcaatcgcc ccgcctgaac tgatctgcaa gagaatgggc atgaaccaca
    30061 aggcgggcca gtaaaacgcc agcacgatgg aaagcacatc ggctggataa aaataacggt
    30121 cgtgcatctt tggcagcaaa aagggaatca acattgccga aacgaatgca agcaaaacaa
    30181 tgtgcttgtc gtccaagtct gtttttgcct gccaggagga gtacatccag acgagagtga
    30241 gtgctgctgt caataccagc ccgaaaggca gaaccaccgt gtaccactca cgctgaaaca
    30301 ggctgtatat attcggcgca ttcatcgaaa gcatgttgaa tgtatctgac tgttttgcat
    30361 acaccagtaa agcgtccatc aatggtctgc cgagcaacac aactggagcg atggccagca
    30421 gatacaccag cggaataacg cccataaccc accagcggat tttttttcga agcaccatca
    30481 ccccgaggaa gggcagcaaa aaaacagcct gagccttcac tgagaacgcg gcgccaaatg
    30541 ccagcatggc aggcagcggt ttttcggtca acaggaaata taaacaggcc agcagggccg
    30601 atgtgtaaat tgaatctgcc tgcccccaat aggcgctgtt caataccacg gtcggcgcgg
    30661 aaaaatagat cgctgccgcc agcatggaca gattcccctg cggatatttc agcctgacga
    30721 tcctgtaaat gaaatatgca ccgagcaggt cgaagaaaat ggggatgagt ttgatcgccg
    30781 tcaacggcgg aatgagatca cgggtcagcg tggcgagggc cagcaaataa gtatatggcg
    30841 gcgcataatt ggagaaattc gtccccagtg cattgccgat gcctttttga cttatctcct
    30901 gataccagcg caggttgtac actgccatat cacgatttga gtttggaatt gcctgtacgc
    30961 gaaatgccac tgatgcaaac accagcagca aaatcgtcat cgtaactggg ttgaattttt
    31021 gtcgcttcat atcagaaaat gaaatgataa atttttcgcg caagctccat gaacatttca
    31081 ggccatacca gcccgaatgt catgtaatac atcaagtaca tccctgttcc acccatgaac
    31141 acagacatgc caatcagtga tttttccatc cagcggattt ccttgatggc gctggccagc
    31201 aggatgaaaa gaaagactat gaaatcggtt gtgtaacgat acccaaattg cacccagccc
    31261 gtggcatgat acatccaaag cgggattaag atcccgatca tccccgccca ggcagggatc
    31321 acccagtcat cacggcgctt cgctcgaaaa atcagcaaaa aagcgggtgt ggcgaggaaa
    31381 atgctcatcc ccaatgggtt aaaaaatttg gataactttc caaagtcatg gctgatcggc
    31441 tcaagatacg agcaggcttc attgacgctg ggaaaaatgc ctgtattcag attcggtaaa
    31501 ccgaaaatcg aaacatggat attgcaaggc atgtactgca cattgaatcc gccgctgcgc
    31561 gcaaaagttt ggatcaacgc gttggaaccg cggacatacc cataaccaaa atccactgga
    31621 ttgccgaagc ggagttggtt gtacgtgagc atcacagcga cgcttgcggc cagcatcacc
    31681 ccgaagggaa tcatcttttt tagcacgggg gtgatggagc gttcgcgcag aacgacgagg
    31741 agcaaaaaga acaatgcaga aaagatcgtc ggcgggcggg caaggaaggc gagacccaac
    31801 cacaagccag ccaaccaggg tctgtttcga atggcatccc gagcatataa cagcatgaac
    31861 aacaaggcca cgatctgtgc gttgaaccag accgatccca cactcgcaag ccaccagtga
    31921 acggtgcctg cgatgaataa cgcgatcaac cagatgcgcg tggagaagtc ttccacaatt
    31981 tggccaagca ggacagccat cagcgccgca tttgttgcgc cgaccaatac accgaaaagc
    32041 acatcactga aacccttgcc gaataagatc acaaacggaa gcgaaagaag cgcaggcgtg
    32101 attcccccgg ggacgtacca tttacctttg aattcgatca ggtcatatct gctttctggt
    32161 agttcaacca ggtcagtatg tccttgcagg aacgagtaag ccagatatac atattgcggc
    32221 gtttttgatt gctgtgtgag ttttattcct gcgcttaatc catacagccc caacgcaagc
    32281 aggaatgcaa agagggcagc gttacgcctc aggatgtctg ctgatttctc ccaaaaattg
    32341 cttttcatct gcaacaggaa caggtttact taccccgagt tgacgcgatg tgcttccatc
    32401 acgttgggca ggttttcaat gcgggttaac acgcggctga gttgagtgag gtctttgacc
    32461 tcaatgatga ggcgcaaatc tgccatgctg cggttgacgt tcactttcac atcggcgatg
    32521 ttgatgtttt catccgccag caggttggaa atgtcactga ccagaccctg cctgtcgtag
    32581 gccttgatct tgatcggcac agggtaggtc cgttctacat gaccccagcc aacttgcagc
    32641 aggcgttcac gttccttcgt ttgcaggatc aaaatattcg ggcaatcctg cctgtggatg
    32701 gtggcgccgc gcccacgggt aatgaatccc accacttgat cgccaggcat cgggttgcag
    32761 caccgcgcca ttgtggagag catccctttc aagccgacca cttcaatggc atttgtggaa
    32821 gtggaaggtg tggtggatgg attgacctcg aagatatcgc tggccgcctc ttgctgtgag
    32881 aactgcttga tcaccttgct gacggatagg tcaccgttgc caagcgcgat gaacatttca
    32941 tcgggcgtct tgtattcgag gtcgcgtgcc atcctttcga agttgatgtc ttgcaggccg
    33001 aggcgctgca gttccttttc gagagcgtca cgtccctgag cgagattttg ctcgtcttcc
    33061 tgcttcttga accagacctt gatcttcgag cgggcgcgct gggtgcgtac cagccccaga
    33121 ttcgggttca accaatcacg gctgggtgcg cctcgttttg cagtgagaat ctccacctga
    33181 tcgcccgttt tcaattcctg atacagcgga accagtttgc cgttcaccct tgccccgcgg
    33241 cagcaatgac cgatatcggt gtggacgtga taggcaaagt caatcggtgt ggagcctgcg
    33301 ggcaggtcaa tgatgtcgcc gcgcggagtg aagatgtaca cgcgatcctg gaacacatca
    33361 ctgcgcatgg attcaacgaa ctcggcggcg tctttcacgt ctgagcgcca ttccatcatg
    33421 gtgcgcagtg aattgatgcg ttgttcgtaa tttctgtctg cgtgcttggt gccttccttg
    33481 taacgccagt gcgcagcaat gccgtactcg gcattgaggt gcatctcatt ggtgcggatc
    33541 tgcacctcga gcggacgctt gtcatcgtag atcacagctg tgtgcagcga ttgataaaaa
    33601 ttatccttcg gtgcggcaat gtaatcatca aactcgccag ggatgggccg ccatgtggtg
    33661 tggatgacac ccagcgccgc atagcaggat ggcatatcgg gcacgatcaa acgcaccgcg
    33721 cgcacatcgc gcaccatgtc gaacggcttg cccttctttt gcattttctt gaagatcgaa
    33781 taaatgtgtt tgggccgccc gctgacttcc gctttgatgt tgttcttttc aagcagcttg
    33841 accagatttt ccttgatggc ttccagctgc gcttcacggt cagggcgctt ttcggtcagc
    33901 agttctgcga tctctttata tttttcgggg ttcacatacc gaaagccgag gtcttccaac
    33961 tcccacttga tctgccagat gccgaggcgg tttgccagcg gggcaaaaat atcgagcgtt
    34021 tcctgtgcaa tgcgtctgcg cttgtgctca ggcatatggc ccagcgtgcg catgttgtgc
    34081 aggcggtctg ccagtttgat caaaatgacg cgcacatcgt cgcccattgc cagaaaagtc
    34141 ttgcgcaggg tctctgaaac aatatcttcc ttgcggccaa gcagggcagg ctcgatcggt
    34201 ttttcttcag tgtgatttcc gttttcgtct ttttcaggat gctggtcacc gcgtgaaacc
    34261 cgcggcaggt gagttaactt tgttacgcca tccaccaaaa ttttgatcgt atccccaaag
    34321 tcgcggcgga tgtccgcgag cgtgatggtg gtatcttcca ctgtgtcgtg cagcaacccc
    34381 gccgcgatca cttcgggcgg cactttcagg tccgataaaa tactggccac tgcaatgcaa
    34441 tgattgatgt acggctcgcc cgagtttcgc ttctgctcgc gatgcgcctc ttcggccacc
    34501 cgatatgcgc gctgaaccag ttcgcggtct gcaacggtat attgctctgg taattgttcg
    34561 atgagttttt caagtggaat ggcagtattc gacgatttca tgcgctttat tgtactacgc
    34621 ggaattaata aaaaacagga cctgcccgct tcccccttaa ccgaggcatg gtcctgcttt
    34681 cttttggcga atcagcgatt ttagagggtc ttgatcaagg cctgtctgct gttgcgcaaa
    34741 cggtctgcga tgatctcggc gatgcgctgc atcactttga agcccgcttc gggatatttc
    34801 tcgagcaatt tgaggaaggg ttgggcgggt gtgatgagca gtttcgagtc ttcatcacat
    34861 tcggcactgg aggtgtaatg atttggcgca acgatgcccg accagccaaa actttgatac
    34921 ggcgtggata cgaacgaaac ggtcaggctt tcgggtctcg aagtcagctt gacgcgcagg
    34981 gctacactgc cattaagcag gaagtgcagt ttgtccgctt tttcgccttc acgaaagacg
    35041 aactcgccct gtttcatttc gacttcatct gaaatcgcgg cgatctcttc cagaagctgc
    35101 tggggtagat cctgaaataa gccgaactgg gataacacgc ttgctgttgt cataactggc
    35161 ctccaataat ttgaatttgc ccgagggctg aaaatatcaa tttcgcagga gcaggtgtac
    35221 ggtcagggtt tgaagcggcg gttcgttcat ccaatttatg cttataattg tacaggattt
    35281 tttgattatg acagtcacca ataatgtcac aagatttttg gatgcgcgca aggtgaagta
    35341 cgaggcgcat gaactcccgc ctgagaaact cggcgcagtg gaggcggcgc agcatatggg
    35401 tgtgccgccc gaacaggttt tcaagaccat cgtggttacc cgtgaaaaaa agaaaccgct
    35461 attgacggtg atccccgggc cgcgggtggt ggatctaaaa aaactggcag cgttcttgaa
    35521 cgagaaaaaa gtttccctgc ccaccgagcg cgaagccgaa gccttgacgg gcctgcaggc
    35581 gggcggcatt tctccgctgg cgctcatcaa caagggcttt caagtggtga tcgattctgc
    35641 ggcgcagggg tttgagaaga tctatatttc aggcggtcag cgtgggttgg atattcaatt
    35701 gcccgtggcc gatctggcaa gtttgacaaa tgcaaaattt ggagacgtaa gcagggacga
    35761 ataaaacgac gctgggttgt cgatgaacac aaaaaaacgc agaccttgca catgacagca
    35821 atgatctgcg tttttgtttt tggagcgcgg acttgagtcc gcgtttattt tagtattttc
    35881 cgtaattcac tggctcactc aacgcgctga ccactttcaa ttgttcgcgt ttttcagagc
    35941 caagtttttg ccagtattct tcgcggtcct tcacaccaaa aacccattca tccaaatagg
    36001 ccttgaccga atcctgtgac tcgctgatct tatcccaggc gaggtaaaac tcgttgtcac
    36061 ggtcgtagta accctgtgag taggatgggt ggcttgcgaa cggaacatgg cagaccgcgt
    36121 ccacaatatt ggcggggatc atcgtgcggt tcgggtcgga ccgaatgacg gattcttcga
    36181 cgatctcttc cgcggtgagg atgacctttt tcgcggcaaa ggcggcttct ttttgttcgc
    36241 cgatgatgcc ccataaatgg gcattgccgt tcttatccgc tcgctgcacg tggacaatgg
    36301 caacatcggg attaagcgca ggcacgacga tgacatcctt gccgccatac gggtcgggaa
    36361 tgcgtttgat gagcgggttc gatctttcga ggtcggttgc tcccgtttga ttcatgggga
    36421 gaaatggcag cccggcggcg cccgcctgca ggcgggtgat cattccgaaa tgggaatatt
    36481 cctcccattc cagttcgcct ttttcaagag cggcgcgcac aattcgcagt gacccaaccc
    36541 cgggattgcc catgtaagaa aagatgactt ttttggcaca gcctgccgcc accatttggt
    36601 cgtagatcag gtcgggggtg gcgcgggcga gggtgagatt tttcctgccc tggcggatga
    36661 tctcatgccc cgctgcaaat ggaatcaggt gagtgaaccc cgccgcgtat accacgtcgc
    36721 catcttttac aaaatctgaa attgcctgtt tgagtgtgat gagtttggtc atgttatttt
    36781 ttcttggcct cttgtttttg ctgtttttta tttttcgcat tttcgcgcca ctttttgaag
    36841 gaggcgaacc gcccagccga tggcgggggt gccattccgc gcctgaggta ggcgccgatt
    36901 ccaaacatca gcagggcgac gaatagaaag tcaaaatcca ccttgtcggc gatatcggag
    36961 gtgacaaaga gaacaaagga cccgccgccg atgacataga agaacatgcc gatgcgaaag
    37021 acaaaatcat cgtccatggg ggtattctac caaaggctgc ttcacttgac cgcagaaaga
    37081 aaggtgcgtt tgaaggggta gaaaacggga ctgcccggta cagccgactt caatttcgtg
    37141 cggacggctt cggtgaaagc ttctttttgc tggcccaatc tttcaaggta gggcaccagt
    37201 gccgtgccgc gaatccactc tacgatggcg tcggagtttt caagtacgtg ggggtatacc
    37261 ttttcgaata cggtgatgtc tttcgcgccg cattcaaaga ggatctctgc atagcgttcg
    37321 atggaaagga cgggcgcctg acggttaaat ccgccaagcg cagtttggaa gggttcttcc
    37381 ctggctgttt cgcggatgat ctggtgggag atatgaaaat gattggaagg cacctgcacg
    37441 gcaagttgac cgttttcctt caacctgcca aacaaacgcg gaatcagcgc ctcgtgattt
    37501 tcagaccatt gcagggcggc attgctgaaa atcagatccc agtcgccttg caattcggac
    37561 tggtcaccct gctcgaatcg cagggaaggg ctttcatgtt gtctggcttt gttgagcatt
    37621 tccaacgaag aatcgaggcc aaggacggtg ccttcaggca gggcctctgt gaggtgtttg
    37681 gtgagctcgc cggtgccgca gccgaggtcc acggcttgca ggttggggcg gatgttgacc
    37741 agtttcaaca ggtcaaagaa aggcgcggag cgttccgctt gaaacttgtg atattgagtg
    37801 gggtcccagg gcatgatgaa tacctataga taattgaaaa cccctttctc gaaaagttga
    37861 gaaaggggtt tgtgtttatg cttcttactt aacgagtgta cggatctgtt cgtgcatgta
    37921 aagcgggaga tagtagtggc cgccagcgtt cttgccgctg gaaccagaac ccttccaacc
    37981 gccgaagggt tggaagccag gccatgcacc agtggttgca ccctgcggac gattggcgta
    38041 ggtgacgccc gcttcgatct tatcgaagaa ccattcggtt tcttcggttg agccgtagaa
    38101 accagcggtg aggccgtagt tgacatcgtt ggcgatgctc atggcttcat ccaaattctt
    38161 gactctgcca atggtggtga tgggcaggaa catttcatgt ttccacaagc ggtgcgagaa
    38221 gggcagatca acaacgaagg tgctttcaca gtaatagccc ttgtcgaaca tgccgccggt
    38281 cttcacgttg cctccgacga ggaacttgcc gccgccctgg ttgatctctt cgcagaagtt
    38341 cttgaaatca ttgtaggatg acttgttcac aacggggccg ttgtaggtgt tgcggtcggt
    38401 ggggtcaccg aggacgagtt tggaagcggc ttctttcaaa cggccgacca ggtcgtcgta
    38461 aacagaatcc tctaccagta cgcgcgaagc ggcggagcat ttctgtccct gtaaaccaaa
    38521 ggccgagcgg atgatgccaa tggtggcgcg ttccagatcc gcattcttcg aaacaatggc
    38581 ggggttcttg ccgccaagtt ccaaaatgat cgggcgcaca tagttgcgtt tgccgaaatc
    38641 ctggaacatc ttcatgccca catcgaagga gcctgtgaag gtgacgccat ccacttcgga
    38701 gctatccaca agcgcctgcc cgagtgtgga gccaggcccc gtgacgaaat tgacgacgcc
    38761 atcggggatg cctgcatcgc ggaaacaatc caccagcagg cggacgatcc aggcggtgtc
    38821 ggttgcaggt ttgatgacaa tggtattgcc ggtcaccagc gccgcgccgg cagggccgcc
    38881 tgtcaacgca aatgggaagt tgaacggaga aacgatgagc cacacaccgt acggtcgcag
    38941 cacagaaaca ttgcgggcat cgtatccaac cagcgggtct ttgcccattt ccacgatgta
    39001 gccatcgttc ttctccattt gatagcagga gtagtaaatg aggtcggcgg tttcctgcac
    39061 atcgccaagg gattccatgc gattctttcc aacctcaagc gccatggctg cgccgagatc
    39121 gaaaatgcgt tcttcgatca gggaagcggc cttgcggagc aactccacgc gcttttgcca
    39181 gggggtgtgg ctccacattg ggaaagccgc acgagccgcg gccagcgctt cctgcgcatg
    39241 tttggcgttg cctttctgca tgacagcaag tttccaatcg gtattgacgg gggagaagtc
    39301 atcgaatttg tcgtcggctt ttacctcctt gccgttgatg agcatggtgt attcctgacc
    39361 catgttcttc tttacttttt ccacggcttt atcaaagccg gtatgcaatt cttcaggtgg
    39421 gttgaacatg gttgcgtatg taagtttaaa gtctgccata aagatcctcc ggggttggat
    39481 tgatggtcaa cggtttttag ctggctgact gtgaccatca tttaacagcc agccaatgaa
    39541 tgaactaata ttagtatagc gcagcccgat gcaatcgtca aagtagagcc attttcaggc
    39601 gcggctaaga atttcatgag attttgtaag aaggcctgaa atcacgctat catattgaac
    39661 ataccatcag gagacattta ttcatgagac cacactttcc agatcggatt caaagacacg
    39721 gttcgttcct gaccgttgtg ctggttgccg tttttgcggc atccgcctgc gccccggcag
    39781 cagcgaccaa cccgcccccg gcggcagaac ttcctacagc aacaaccgcc cccgccgcgg
    39841 acgcgattcc caccgccaca ggtgaagtgg ctgcccctgt tgtgcccact caggccgttg
    39901 cagaagtgct gcctgttgcc acctcccgcg gacccaacct cgaagccact gaccccacca
    39961 cagtcaattt ggcggctggt cagttacagt tggtggaatt tttccgcttc acctgagcga
    40021 cctgccgatc catggcgccc atggttcatg ggctcgaagc agagtatttt ggcaaggtca
    40081 agttttcata tctcgatgct gatgaccccc gcacagacgg attccagcgc gcgctcggtt
    40141 tttattacca gcccgaactc tacctgttgg atgcaaatgg caatgtcttg aaaaaattca
    40201 ttggcctgac ctcccgcgaa gacctggtgg ccgaattcga aaaatatctc caataagttt
    40261 ttgtgtgtaa aattgaagca ggctcaaccc aaagttgagc ctgctttttt gatgctataa
    40321 ttttttgcaa tgaatctgct gaataaaaag aaagaggttg tgtcatgcga attggcaagt
    40381 cttctctgtt tttagtattt gcattggtgg tgttgacctt gccaaccgcc tgcaacctgg
    40441 gtgttgttca gccagaggcc agcccgccgc cagttgccat cgttgaaaca ccaacttttg
    40501 tcattcctcc ccctgttgaa ccgacagcca cccccactct gattcctacg atcgtgccca
    40561 ctgcaacggt cacacttttt catagcagca ttcccacgct cactcccacc tcacagtgga
    40621 cgggctgccc cggtattgtc gtcaaagtgg atgacaccaa aaagggagat attctcaaca
    40681 tcctgcgctg tgacgatgga ttggaatatc aacttggccc gctggccaag ggggtgtatg
    40741 ccgttggccc caacaataac ttccttattt acgtctcgtt cgatggtatt gtctacgccg
    40801 cacggatcgg ggacagatat ttgaatccct tgtataacct tggccgtgaa catatcttta
    40861 ccgtgttcaa taaaaaagtt gcgcccgatt tccagctttc ctttacgggc gaaggtccga
    40921 tctacagact ggtcattctc gaaaagaatt acgatcaaaa acggatgtac gaactgccaa
    40981 tagggttgac ggaataaatg ggtatcttca tcaaggtgtt cgtttttatt gcagggctgg
    41041 tcgtcgtagc cctgacggtt ttctcggctc tttccacctt tgttttgccc cgcgctgccc
    41101 gcagccagtt gaatcgcctg gtgtttgggg tcatgcgccg cgtctttgaa ttcatcctga
    41161 ggttttttcc aacctacgaa cagcgtgaca gtatcatggc ttattactcc attcttgggg
    41221 tgatgatgct ggtgccggtc tggtattttc ttgtggctgc ggggtacgcc gccatgtatt
    41281 ggtcgcttgg cgtgggagat ctgtatgcag attttcgttt cagcggttcc tccgtcttca
    41341 cgcttgggtt tgcaacttcc gataatccca ttgtcacctt tttgtctttt tccgaggcgt
    41401 tgatcgggct tgtgctggtg gcgcttttga tggcctacct gccgaccatg tatgcggctt
    41461 tttcacggcg tgagcaggct gtgaacatgt tggaggtccg cgcagggaat ccgcccaatc
    41521 cctacgatat gctttcacgc ttccaccgca ttcacgggtt ggataaactg ggcgatctgt
    41581 ggaagaactg ggaaatctgg tttgccgatt tggaggaaag ccacaccact ctgcctgctt
    41641 tggtcttttt ccgctcaccg cgcgcggata attcgtgggt tacctctgcg ggggccgtgc
    41701 tcgataccgc cgccctgacc ctgtcttcga tcgatattcc aaacgaaatg tctgctgcgt
    41761 tgtgcatccg cgcggggttt atcgcctttc ggcgcattgc cgattatttc gatattacac
    41821 atccacagaa tcccacctac cctgaaaccc ccatcagtgt ttcccgcgca gaatatgacg
    41881 aggtcatccg taaattggaa gcggcggggg tgcccatcaa acccgaccgt gaaaaagcat
    41941 ggagggattt tgcgggctgg cgcgtcaact atgaccgtgt gcttttggtt ttatgtaccc
    42001 tggtcatggc gccgcaggtt ccctggtcca gcgaccgtgc gccgaagttc aaaatgccgc
    42061 cattgttcgt cccgaaaaag aaactcaaac attaaaccaa aagcggactg gcgttagcca
    42121 gtccgcttac tgattaccga ttactgtttt ccactatcct tcatacttct ttgcatcttc
    42181 cactgctttg ctcacattca cgaacggcag caggatcaag cccatgatga agaagaagat
    42241 caacgacagg atcgcatagc gcaaattccc aaagatctgg ttggcaagac cgaacaccag
    42301 cggaccgatc catgaggtgc cgcgttcgct gatctcgtag aaagagaaga actccgcttc
    42361 cttgccgttg gggatcatct gggcaaagag actgcggctg atagcctgtg acccgcccat
    42421 tacgatcgca atgaaaacgc caaggacaaa gaattgcgcg gttgcacttt cacctttcaa
    42481 accgccgtag gcgtagatca ccactccagc ccagatcacc agacttacaa ccaccgattg
    42541 cttggcgccg atccaacctg ccagcttgcc ccagaacaag gcaccgaaga acgccacgaa
    42601 ctggatcatc aaaatcacgg cgatcaaagt cgattgttca agttccagcc cgccctgaat
    42661 cagaggcgcg gcggcaaatg tcgaagcgac ggcgatcacc gtctgaattc catcgttgta
    42721 gagaaaatat gccagcagga atttcaaggt ctcagggaaa tgcttcactt cgctgaaagt
    42781 ctttcgaagt tgtttgaaac caatgctgac atacgtatcg cctgcgggca gttgtcgcgc
    42841 cgcatggcgc gggcgcagtc tgctccatgt gatgagcgaa aacccaagcc accagatacc
    42901 cgccgacgca agattgatcc gcaccgccaa ctcactgggc acgcccaaat cttcggcaaa
    42961 ggtaaagaaa gccaggttga gcgccagcaa aatgccgccg cccatgtaac ccattgccca
    43021 gccataggaa gaaacacggt cggtttcatc ggggctggca atgtctggca gataggcatt
    43081 gtaaaaaacg atcgccgcgc caaaggcaag gttggcaaca ataaagagca acccgcccag
    43141 ccaccacaat ccacctgtta cgaggaacat caacaaggtt gcaatcgcac cgatggtcgc
    43201 aaagacctgc atcatgcgtt ttcgcaaatg cgaataatct gcaatggcgc cgaggatggg
    43261 caggaacaaa acctgcatcc ccacggaaaa cgaaatgcag tacgggagga acgaatcagg
    43321 cgcgacggga atccccaaaa acgaaacatt ggttgtgcct gctgcttccg ccgcggagcg
    43381 cgccagcccc gccacatacg gcccaaggaa taccgtaccg atggtcgtgc tgaacgctga
    43441 attcgcccag tcgtacatgg cccagccaaa gatctcccgc ctgtcattta ccattgcgcc
    43501 ttcttttgcc ataactcctc catgagttaa aaagtggatg caagcaagaa cacaggcgag
    43561 tataaggagt ttcggtttca atgtaaagaa atatatgcag atgaaacccc gcctcctgct
    43621 tgaccttgaa cctgctacgg gctaaaatgc tgttcctact ctggagcatt tacccatgtt
    43681 tgaaaccctc actggacgac tcaaccaaat cttcgacaat ctccgccgcc gcggaaaact
    43741 ctccgaagcg gatgtggacg ccgcgatgaa agaagtgcgc ctcgcgctca tcgaggcgga
    43801 tgtgcatttc agcgttgtca aaacctttat cgcccgcgtg cgcgaacgcg ccgtcggtgc
    43861 ggaagtttca aaggcgttga accctggcca gcaggtcatc aagatcgtca acgaagaatt
    43921 gatcgcatcg ctgggcgagc ccgtcggatt aaatctcact ggcccgaaac cgcgcgtcat
    43981 catgatggtc ggtttgcagg gcgctggtaa aacgaccgcc tccggtaaac tcgctcgctt
    44041 aatgaaagca aagggcgaac gtgttttgct tgtggctggt gacccatacc gccctgcggc
    44101 tgttcagcaa ttgcagcagc tcggcgaacg gctggatgtg gaagtggaag ctgatctttc
    44161 gctcacacct ccccagcttg cgaaacgcgc cgtagataaa gctgaaaaag gcggattcag
    44221 catcgtgatc gtggatacgg ctggccgctc tcaaatggat gcggcattga tggatgaatt
    44281 gaaggccatc gccgggaaca tcaaccctgt ggatatcctg ctcgtggtcg attccatgat
    44341 cggtcaggaa gcgttgaaca ttgcgcaagg cttcaaggat gcgatcaacc tgaccggctt
    44401 gatcctcacc aaaatggacg gtgactcacg cggcggcgct gcgatctcca tccgctcggt
    44461 aacaggggtg ccgatcaagt tcatcggtac aggtgaaaaa ctggatgcgc ttgaaaccta
    44521 cgacccttcg cgtttgtcct cgcgcatttt gggcatgggc gatctgatcg gcctcattga
    44581 aaaagccgaa gccgcctatg acgccaaaga tatggatgcg caagcgcggc aggctgaaaa
    44641 gatgatgaag ggtcacttct cgttggagga ttggctcgac cagatgaagc agatgaagaa
    44701 gatgggtccg cttgcacaga tcatggagat gctgcccggt cagatggggc aggccgcccg
    44761 tggcattgat ccgtctttgg tggaaaaatc cttcaaacag tcggaagcca tcatcaactc
    44821 gatgactttg aaggaacgcc gtaatcccga tattttgaac gcttcgcgcc gccgaagaat
    44881 tgccgctggc gcaggcatgg aagtgcagga tgtgaaccgc ctcatcaagc aattccgtga
    44941 agcacaaaag atgatgaaga tgttccaaaa gacgggcggc aaggggttgg ggaaattgtt
    45001 cgggtagatc atttcattgg aaaatgtagg gacacagcgc cgctgtgtcc ctttttnnnn
    45061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    45121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatca tacgcaacgt aaacgttgcg
    45181 gtacgaaatt ctacttcttc ggcaggcacc gttgcggctc ctggcctgtg ccggtgaagc
    45241 cttgaacttc gcggcagacg ttatccacgc cgaagagggc cttgagcgga tagaacttgt
    45301 cactgcggac gggtgagcct gcttcttctt ctgtatagcg gtctacgtaa tcgaggtcgg
    45361 cgatgcttct tgggcctgca tctgcaagta cgccgtacat gaatttattc tcagcgaccc
    45421 acaatttgaa ggcgaattgc accgtggcat acctgcccgc gttgatgcgg acccacgcca
    45481 aatcagagtc gttgaccgac ccgaggccgc tgtcgaagat gatttcattg tagccatcac
    45541 cggggtaggg agcatctgat ttttcggcgg agaggccgcc cgtgtcgcga tttgaatctt
    45601 tggcgaccac cacaccatct gtggaccatt ctgcattgta gggcggtctg gcggcgatga
    45661 tgtagtcacc gaaaccgtcg gcatccagat ccagttcgac ggcgtagttg attccgatct
    45721 cgttgttcgg attcttgccg acgagttcga tcgagacgta ataaaatttt tcgtcgtgac
    45781 tcaggttgta gctgacgatg tccacatcag ggatgtaggt catgtcctgt gtgaaggggc
    45841 gttcgaagag gttgatgttg tacgaatcac catagggggc gcggttttcg gcggcggtat
    45901 ccacggaaac cacgtcgtgg ataaggtcac ctgctctgat gttcgtggcg gggacgaggg
    45961 aatggaaaac agcaatggtc ggcgtggggg ggaccactgt cggggaggga atttctgttt
    46021 cggtggggac agttgtcggt gtggcagagg cggacgccgc aggcgtttcc gttggcagaa
    46081 tgggaacggg tgtggcttgt ggggtggcag agccaggcag gttgcaagcc agcacggaca
    46141 aaataagaat gatgctgaag atggcagtgg gattcttggt tttcatatgt ctctcctcgt
    46201 aatccatttt atccctccct tgtcagacgg gcaatcttcg ggcacaatag ttcctgacaa
    46261 ccgtaagacc gacttaagtc ggtcttacaa aaacatagag gaaccatgga ctattcatcc
    46321 gctgaattaa tgatcattaa cgccgcgcgt ttgctgcgcg atggcgatgt tgtttttgtt
    46381 ggggtgggac agccgaattt ggcttgtaat ttggccaagc gcacacacgc gccgaatctg
    46441 gtgatggtgt acgaagcggg tgtgatcggc gcggagcccg cccgtctgcc gctttccatc
    46501 ggtgacccga ccctcgtgag cggttcggtt tcggtggtga gcatgtacga tatttttgca
    46561 aattacctcc aacgcggcaa cgtggatgtg ggtttcatgg gcggcgcgca aattgacaag
    46621 catggaaata tcaatgccac ggtgatcggc ggatatgacc atccgaaagt gcgtctgcct
    46681 ggttcgggcg gttcgcagga gatcgcggcc tgggcgaatc gctgttacat catgaccccg
    46741 catcaaaaac gccgcttccc tgaaaaagtg gaatttatga cctcggctgg attcatcggc
    46801 ggaaaaggcg accgcgaaaa ggcgggcttg cgcggcaagg gcatggtcgg cgtggtgacg
    46861 gacattggca tgttggaacc cgatgaaaat ggcgaaatgg ttttgaccgc gctgcatcca
    46921 ggcaagaccg ttgaagacgc caaagccaac acaggctggg acttgaaagt cgcggcggaa
    46981 gttcgtgtca ctgaacctgt gaccgaaaat gaattgagaa tcctgcgcga agaacttgac
    47041 ccgacgggga tttatttgaa gagcggcgga tagatctgtg gtaaatattt tgtctcgtaa
    47101 ccagcaggta taatcggcaa aatgaaatct ttgaaaatca aactgccagt tgggcagg
//
LOCUS       DAOQLC010000016        43992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_240_length_43992_cov_21.291463, whole genome shotgun sequence.
ACCESSION   DAOQLC010000016 DAOQLC010000000
VERSION     DAOQLC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 43992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43992
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_240_length_43992_cov_21.291463"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..613)
                     /locus_tag="PL000_04570"
     CDS             complement(122..613)
                     /locus_tag="PL000_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88200.1"
                     /translation="MSMNKPVSASTPVDTAPYSAQNQNAPRVYVGLTLTFVGLFIFTI
                     GSKPDWFGWDRSAVVGFVQIAVFLVGLALICLGGYISLLTLWHGQERTILADIGTRLV
                     GTGYVIAVFAGLADVFGMGSQSFPKVPYFGPWQATGVLIGQGVIALGFLLFIPFFPAR
                     KKN"
     gene            complement(610..1707)
                     /locus_tag="PL000_04575"
     CDS             complement(610..1707)
                     /locus_tag="PL000_04575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNC88201.1"
                     /translation="MPIIVDAHADIAWNMLKYGRDYTRSAVETRQIEAGSDVVKQNDD
                     TLLGWPEYQNGQVAVIFSTLYATPIRWSKSENKAQVYKTYDEAHQLYREQLLTYHRMA
                     DAMPDKFRLIASNRDLDLILENWQSLPATASSGHPVGMVVLMEGAEGIRTLSELEEWH
                     EMGVRLIGPAWVGTRYCGGWNEPGPLTPDGRKLLAAMADYNFILDLSHMDEQAAVEAL
                     DIYRGPIVGTHANCLSLMPNSPSNRHFSDRIIEGIIERDGVIGVVPFNGYLKVGWVAG
                     KSERSEVSLDHVANHIDHICQIAGDSLHAGIGSDFDGGFGLQSVPPEIETVADLQKLS
                     ALLLARGYNDVDVSNILGGNWLNRLRRDLPS"
     gene            complement(1756..2976)
                     /locus_tag="PL000_04580"
     CDS             complement(1756..2976)
                     /locus_tag="PL000_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNC88202.1"
                     /translation="MDQNEAQTGQGAVNPQGDQPHNQTMESLLESESLSVELPQVGEI
                     RKGMIASIGPSQILISVGAKSEGVVAGRELEQLTAEERAALKVGQEVNVYVLNPEDQN
                     GNVVLSFKRAQEEMSWENVEKMIADETVIDTKIVGFNKGGLIAAVGNLRGFVPSSQIS
                     AVRRAQSSGDTPEQRWQKMVGQAISVRIIEVDRERRRLILSERSTNTETRSSMKDRVI
                     SELEEGKVYTGKVTSLADFGAFVNVSGADGLVHLSELSWDRLQHPKEILEVGQEVKVK
                     VINIDRDKKRIGLSIRALQDDPWKSRVEKFSVGQLVEGVITRLTKFGAFARLEGDIEG
                     LIHISELSENRVEHPKEVLKEGEVKTLRVIRIDGEQHRIGLSLRKVDSAAFADKDFKM
                     LTQGFQDPDEENKE"
     gene            3119..4219
                     /locus_tag="PL000_04585"
     CDS             3119..4219
                     /locus_tag="PL000_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88203.1"
                     /translation="MPAIFPFTAIVGQERMKRALILNAVDTRIGGVLIRGERGTAKST
                     ASRSLAALLPHVKIVQDCRFGCDPDKSSTWCTECKERYAASKKLPIAERATPFINLPV
                     SATEDRVVGTLDIEKAIQKGERHFEPGVLAGANRGLLYIDEVNLLDDHVVDILLDSAA
                     MGVNTVEREGISFAHPARFILVGTMNPEEGDLRPQLLDRFALSVDIVGIRDARERVTI
                     MERNLSYEKDSEAFRKQWLPKEEELSSKIARARELVDQVTHTSRDLLSIAALTASLNV
                     DGHRADLVILKAARAEAAFEGRTKINDHDIALAAELALPHRIKRTPFQQAELTTEQLQ
                     ERIEQLAGQSMPEDSDEKSESKQDGEGEEKKT"
     gene            4270..5181
                     /locus_tag="PL000_04590"
     CDS             4270..5181
                     /locus_tag="PL000_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC88204.1"
                     /translation="MQQQEVFASSNANWWEGGQKVKAGETFQPRKLNTPLDKLTRKQA
                     GRRSLTKTERKHGRYIKARPAGDKLNDIAFDATFRAAAPFQKQRTEERKKLAFSIKKS
                     DLQRKIRVKRVANLVLFLVDASWSMAVAERMNATKGAILSLLTDAYQRRDRVGLIVFQ
                     KDRATLVLSPTNSVQLAQRALMDIPVGGKTPLSAGLFMAHDVLTHEKHTHPDVEPLLI
                     VLTDGAGNVSLGTLPPQEESHKFAELIANEKIRSVVINMEHAAFDQGLAQELANNLKA
                     PCYALSELKAESLYNTVIDEMSKPAKK"
     gene            complement(5453..6868)
                     /locus_tag="PL000_04595"
     CDS             complement(5453..6868)
                     /locus_tag="PL000_04595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC88205.1"
                     /translation="MIFSNTPRPETLKSLADVKHVPFWLDDPARPEPASALTESITAD
                     LVVIGAGFTGLWTALLAKQADPSREVVLLEAGETGIGASGRNGGFVASSLTHSFQNGL
                     NRWPKELKKIIELGHKNLDGIEATIKQFNIDCDFIRSGEINIANEEYQIEELREEAEH
                     SAEYGEHVQFLDREQIRARVNSPTYLAAIHDSSVAMVNPARLVWGLRRACLEVGVRLF
                     EGTQVTSLEEKRDSILVKTSYGQVESKRVALATNAFPPLLKRLSFYVVPVYDYVLMTE
                     PLSKEQRDAIGWHGREGVSDNGNQFHYYRTTADGRILWGGYDAIYYQNNGVAPHLENR
                     PETFARLAEHFFQTFPQLTGLRFTHAWGGVIDTCSRYTAFWGQAYGGKLAYAMGYTGL
                     GVGASRFGAQVMLDLLDGKKTERTELQMVKSKPFPFPPEPFRSPIVNFTRWSLNRADD
                     NGGQRNLWLKILDALGLGFDS"
     gene            complement(6979..8043)
                     /locus_tag="PL000_04600"
     CDS             complement(6979..8043)
                     /locus_tag="PL000_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC88206.1"
                     /translation="MKNQTFLITVFAAFVLSLGFLSVSDAFAHPSAQSEETPTPSSDR
                     PPVPAEIMEGVNNLRLSHGIAPLNAHPVLMQIGQMEADGIAAGREGHWRPEGITLGQW
                     LLSLGYPLSGDISLDGYRSENWGFAHNADEAVAMWLSDDEHENTMLSLERSDIGVGIA
                     KMPDEDVYVIVLETALQTKSGQMQYDAIVYLTGIPMTQAAYSSMATLAAENGLLPQNV
                     APVFLNTAMPDGNVYHEVKYGQTLWSIAISYGTTIKQIQQLNGLTDTNLRTGQKLLVL
                     KGATQPAPGGASTAPTLAQSPQTFPTPTMVPAKTAEPEPTPTLTPEQRRENMMGIAAI
                     GVAAIFLGGLFTAMTRKKPL"
     gene            complement(8063..9931)
                     /locus_tag="PL000_04605"
     CDS             complement(8063..9931)
                     /locus_tag="PL000_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88207.1"
                     /translation="MKRFLLGTLIFVLAACSGAVPTSAPAEPSFFEVTPAPADLKLDS
                     ASEEIQRALLESAAQWTTLQMDGTITTYLPDGTTQTFREQVWLEPLKSRFKVEVNGIT
                     DPAQHGLKLIDGVNLYDANLSLAQVQIAPVPDFIRVGQYIPPLVPGQVNTNPIWTQLG
                     TPLSQLAFASDFSQNGGIFKPLAAESIAGREALVVEWTYDANSLPSLKMWVDTQTGIL
                     LKLQEFTKGNSSQLAGERVVDHISFDVTFDETLFTPPAGLQQAGLPNAEAEAVPVVTA
                     SGSPSSEDAGELYFFLQPRQAGQSIQLARVSGICVFDSANCPPMQIVTVPFAFNFTIN
                     ALSWSPDGKLAAFSYSDSSNGTPTKLWIFDPAANTWTSIAQFAYIDPPFWSPDGTWIA
                     FRTLDGLGGEDVYVVRRDGSELKSVSLNLPVEGRPYIMDGWYTDHILMRSALPSNAGN
                     IFLVRASDGAAKPMFETQQSKSQFVASPDASLLAYDEIDYNTQGHVIKVMEPDGANAV
                     ALTSFAGGSIYPLVWSPDSSLIAFNHYSNSADGSPIAEVHVISRDGKTQSLVYTGTTI
                     GRLLFSPNGRTLLVEETTSISGGHLFLIDLATLEQKILQAPGLSTDFDWYAPSWRP"
     gene            complement(10034..12241)
                     /locus_tag="PL000_04610"
     CDS             complement(10034..12241)
                     /locus_tag="PL000_04610"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC88208.1"
                     /translation="MKFTSKFAAVLLLLVFMFSVVPSAATQPAQAAACYWAQFIADVT
                     IPDGTNFAPGTAFKKTWRLKNIGTCAWNAGDVSLIFDSGEKMGAPASSALPTTVNPGQ
                     TVDITVDMVAPSAAGHYFGYYKFKSTQGGVFGIGSTANKSFWVEINVTSSSGTGYDFT
                     SNAASATWSSGAGTLTFPGTDGSANGFALKLDKPKFENGIESAQAGLLFVPNNVNNGY
                     IQASYPAFKVQSGDRFQATIGCEYGATSCYVAYRLDYQIGSNPVKTFWTFREKYEGLT
                     YNANLDLSSLAGQDVKFILVISAYGSQVGDRALWGNPVIARAGGTVATNTPTVTGTLP
                     TTVASKTPTPGPNACDRAQFIKDVTVADGTTFQPGTTFAKTWRIKNVGTCTWTNYSLM
                     FDSGEKMGGPDSALIPTTVAPGQTVDITINLTAPANAGTYRGYWKLKSSTGVPFGIGS
                     DGTKSFWVEIKVAGTPVTPSTPATPVTPGTPVTPATPISGTSYDFVSNVCAATWYSGA
                     GTLPCPGTDNDAKGFVLITNPSKLENGTTDNRSGLITFPQNINNGYIQGIYPSYKVKS
                     GDKFRAVVNCESGATSCYVVFRLDYSVSGSSSIQTFWAFVERYEGQYYTADIDLTPLA
                     GQDLKFILTVLSTGSATGDRALWVSPIIYNASSGSGSSSASPTPTFTVSPTLQATISA
                     TATTPSPTSTVTVTVPAATATSTPGTPTPPTPTLTLTPDVTATPIPPSATPTP"
     gene            12422..13585
                     /gene="dinB"
                     /locus_tag="PL000_04615"
     CDS             12422..13585
                     /gene="dinB"
                     /locus_tag="PL000_04615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNC88209.1"
                     /translation="MPRTILHLDLDAFYCSVEETQNPALRGKPFAVGGKPDERGVVAS
                     CSYAARALGIRSAMPMSRAVRLCGNLIIIPGRHKLYGEYSEKVMDKLRNLTPLLEQIS
                     IDEAFLDISDIRESPARIALDLQAGIKNELHLPSSIGIASNKLVAKIATEVGKKSSKK
                     KNEPPFGLTIVPAGEEAAFLAPLPADMLWGVGPKTSARLTELGIRTIGDIANWPEKDL
                     VHLFGENGRDLWRHAHGIDNRPIVTEYETKSISQETTFNVDVRDDKTLEKTLREQAKE
                     VARQLRKNDLAGKTVKIKIRWPDFTTLTRQVTLPTATDSEDDIIKAALKLLQAIRKPN
                     QAVRLLGVGVSGIGAPVRQLSLWDAGSEKSRKLQEVVDQLQEKYGRNVIHRGE"
     gene            13672..14637
                     /locus_tag="PL000_04620"
     CDS             13672..14637
                     /locus_tag="PL000_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNC88210.1"
                     /translation="MSNVATRLITLILTLQSQPNQKASDLANKLGISVRTLHRYFSML
                     DEMGIPVYAERGPYGGFSLVRGYKLPPLIFSVEEAVAVYLGTNLISEAWGKLYKDSAQ
                     SAMAKIENILPNEQRDEIHWARRSLVTTSLYRADLASLSPIMEKLRSAARELRQVNIT
                     YQGQTNAKAEKRRIDPFALVFRAGWWYLVGYCHLRNAPRTFRVDRIQKLSVLEKSFEA
                     PADFNIHAYLENEFKNQPSIRAKLRFIPEAAHMVKANPSMWETVTENSDGSMDVTLTA
                     PDLPWLASMALSFAHWTRVLEPQELQVMIREWAQATADLYSEITR"
     gene            14646..15140
                     /locus_tag="PL000_04625"
     CDS             14646..15140
                     /locus_tag="PL000_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNC88211.1"
                     /translation="MSKPDSKDQMLKAAQEERDALEALLNTLTTEQMTQPNIVGEWAI
                     KDVLSHLMEWEKMVMSWYVNGLKGKPTFVPSREFNWGQLPQLNHAIYLKHRDKNLADV
                     QKEFKASYKKIIKTIEGIPEKELFTRKIYAWTKNNLLAAYFISCTSSHYRWARTVIRK
                     ATKS"
     gene            15155..15799
                     /locus_tag="PL000_04630"
     CDS             15155..15799
                     /locus_tag="PL000_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNC88212.1"
                     /translation="MKTLDLKKQFKYLYQPSAKKIEAVQVPNLQFAMIDGAIEKGSEP
                     GKSPTFAEATQALYSLSYTLKFMLKKRKTNAVDYPVMALEGLWGVEDGFFDIAIKDNW
                     TYTLMIMQPDIITSEDFEEAREQVRKKKGDSEMLSKIRLAHFEEGLCVQTMHIGPYAT
                     EPATIDRMKEYMAANKLKDNVGPNGMHHEIYLSDPRKASPDKMKTVLRHPVIAL"
     gene            15845..18232
                     /locus_tag="PL000_04635"
     CDS             15845..18232
                     /locus_tag="PL000_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88213.1"
                     /translation="MKNRFALFILIAVLIGSCAPTPSNTATASLPSSTVIADSPTATI
                     APSATPTPVPITLPVDRFNPIPELNPITTENIDALEEVAFFDNSPISTHITEDKSRLT
                     VWFESGLQTFDMPTLTPYPFIRMNMSIFSSNDAISEDGRLFARAYPGVKAEFWTPTIF
                     IWDTETGEEVCKIKLSEHRGNFFYTNFHLEQNTFSIHGDLGPNGKHKVIIYGLNTCQR
                     IFDRDSDSAMSAISPDGKYAAVTQKGQVVIYDTSTKSHNAIGETRGAQGVAFLPDSGE
                     VAISYSGQTFLYDMTSGEVNGFFDAGISNFYSTISVLDHGEWVVISASEKNYFWNSSE
                     KQVYSVQEFIGRDFKFSNGILTTYKTVFNLRTKAKISIENYGPQASTALSSDQSYLAV
                     SSNIPPFVTDIYETATGKQINSIKGVYGPITVGSDSFIASSHEVGKVFNFITGESDQD
                     LDHSYLSGFLLDEKEIVTWDALGKISLLDIKTGKAVTAANLPVFPSSFLNYTRYKNAT
                     PLWDELSQSDFASLPPSNTMVSNDRTIGIQENNGQLQIFALSDGNFIPATDDPIAAYN
                     LTNLGFQFKFSPDDTIIAGANYNSLTLWDGQTGDQLERIGLPNGAVTDIRFSADGTKI
                     VISKNDGPPQINAFGNLSLIIFDVLEKKIVQDYKLNQQLNKTGCNTTLPFALTSDGGR
                     VVTLTQDCKIGVYDSLTFEELSSFGSPYSDSNPAFSISPDNNLLVVVDRKNIEIWDIK
                     TEKRIRKMDIPELAGTFDFYAHKAAFTSDGKMIIVNSTFWSQSSVTTFWAVPSIQ"
     gene            18273..18632
                     /locus_tag="PL000_04640"
     CDS             18273..18632
                     /locus_tag="PL000_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88214.1"
                     /translation="MNCFYHPDQPAVGLCKYCQRGLCLSCATVVDDVLACKDRHEDEV
                     HQLEQLTARNLFQSKRVASGYMRNAIFYGLVGAAFTGFGLWQLKWLGLQAVVFIMLGV
                     FLFYAAGANYLEARKHK"
     gene            18634..19380
                     /locus_tag="PL000_04645"
     CDS             18634..19380
                     /locus_tag="PL000_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNC88215.1"
                     /translation="MGTWICHLRIAEKLLTHFPELDKTLFTFGNLAPDSGIPNETWTE
                     FDPPKEVTHFLQKGEGEDLIRDLVYYREYLENLHLHDDPQKYSFRLGYFTHLICDILW
                     MKYIGSTTKIAFHELFEQDKQKAVDLVKNDWYGLDQLYNRAHPENLFWQVIYKTPNPP
                     SYLPFVQEKALHQQYAHIRKFYGEPDSEWFIERPYPYLSESSMTRVVEDTVQATLIIL
                     EKIKYTDINKTQSSTEILPQEFMRPYSTPL"
     gene            19414..20670
                     /locus_tag="PL000_04650"
     CDS             19414..20670
                     /locus_tag="PL000_04650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC88216.1"
                     /translation="MNEFENFFNLSTDPMTIADANGKIIHANSVYTAITGWSVDELKA
                     TSYWDLLHPDDHPKARAALQTVKAGHPVFALEYRFLCKDGTYKTFIANLSGNKKSEHL
                     YAISRLKEGQIVPYIMVANAAPVAVIIINTSGTIIHANDLATTLFGYEAGELQEKSIE
                     DLIPMRLRKKHQAHRGEYQSNPVNRPMGSHYNLKGLQKNGLEIRVDIALNPLYEQNEL
                     YVACSILDITEKITTAELALNLERENLRLARLAQRDPLTQAYNRRTMEEMFPKIAEEC
                     KKQNRSVSAIMLDLDNFKTFNDSYGHQTGDQLLKELAYIAQNHIRENDILVRYGGEEF
                     FIIMPNCTPHQASEVAERIRSSLEKDMTSGHKITASLGISTYGFSNGPGEPLDEILEK
                     LIEQSDQALYQAKDKGKNRVQHFSSI"
     gene            20728..20919
                     /locus_tag="PL000_04655"
     CDS             20728..20919
                     /locus_tag="PL000_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC88217.1"
                     /translation="MNEVKTLTRSKSNRMIAGVCAGLADYLNMDPTVVRLLFVIGFFT
                     LNGGMLVAYLIMAIVTPEQ"
     gene            complement(20954..21859)
                     /locus_tag="PL000_04660"
     CDS             complement(20954..21859)
                     /locus_tag="PL000_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88218.1"
                     /translation="MTEAVITVQNLTRRFGDFIAVDHINFEVQAGEVVGYLGPNGSGK
                     TTTIRMLLGLLKPSDGKATVLGYDVFKESEEIRMRVGYMSQKFAIYDDLTTLENLTFY
                     GGVYGITDKTRIQRTLELVGLNGHEHTLTRDLSTGWRQRLSLGIALVHEPKLLFLDEP
                     TSGVDPTARRAFWDLIYELAESGVTILVTTHYMDEAEYCERVGVMRDGKLLAMDTPTA
                     LKHSVITGDVWEVYAYPLEKGLEILSSVQGVERVGLAGDHLRVISSQVEKEAMQSLLS
                     AKSLRVEKIERGEATLEDVFLSLAR"
     gene            complement(21852..22823)
                     /locus_tag="PL000_04665"
     CDS             complement(21852..22823)
                     /locus_tag="PL000_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88219.1"
                     /translation="MNDYLVQANGLVRHFGSVHAVDGVDLKIKPGEIYGLVGSDGAGK
                     TTTMRLLVGALLPSQGEITICGYDVLKQAEQARSTIGYLSQRFSMYEDLTVLENIRFF
                     AEIRGLSTTEWLPRSMEILEFVGLEKFKDRRAGQLSGGMKQKLGLASALVTRPRLLLL
                     DEPTTGVDPVTRQDFWQLVIKLVSQPVSSQSETLQSEGVSVIISTPYMDEASRCHRVG
                     FMKKGKIIAEDTPSSLRSRLNGRILELRGNPINTLRHVAHQDSGVEDVAAFGDKLHVR
                     VLEGQADLIMKRLPEEIKAAGGTLTDLRIIPPALEDVFIALSEVSND"
     gene            complement(22883..24187)
                     /locus_tag="PL000_04670"
     CDS             complement(22883..24187)
                     /locus_tag="PL000_04670"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC88220.1"
                     /translation="MNHKSIPVPVRIVLALVVLGAIGYLAFLTLNTNGNGELTASGSI
                     EAVIVNVSPELAGKVTGVDVAEGALVNANDPLLHLDPSLLTAQRAVAASALDSAKAAL
                     TTAQTKFDQTLQAALSAQDAQRAKDLRVSAPDEFNQPLWFIDQTDQLAAAQTEVDAAK
                     IALDEANSNLEKVISDLGNAQYLDAEKKLSEARAAFLIADKVKVQAENAVEAGAVQDA
                     AYDYYNDVLDELQRAQDDFNALTNTEAEDDIKYARGQVVIAQQRYDAAYTRLLSLQTG
                     TESPAVISAKNTLDQAQTAVDQAQANLDLLDAQIAKLDVYAVMSGVILSRNVEPGEFV
                     QPGAVALTMADLTNLTITVYVPEDRYGQVSLGQTAEVSVDSFPGLTFTATVVHIADQA
                     EFTPRNVQTVEGRSSTFYAIKLQVSDPDGKLKIGMPADVVFK"
     gene            complement(24239..25366)
                     /locus_tag="PL000_04675"
     CDS             complement(24239..25366)
                     /locus_tag="PL000_04675"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88221.1"
                     /translation="MLNSRISSIIRKEFLQILRDPRTLAMIIIMPIMELFLLGYAATT
                     DVKNISMAVWDQSKTTQSRALLDAFRAADYFILEYSVGSTDEYQALIESGDTRVVLVI
                     PADYDRRLIEGNAQVLMVLDGSDASVGATALSTARLVGQAYSTKILSEQTLQSGRGAP
                     SAPLEVRTQVWYNPDLDGAYFNIPGVIGMILSFITSILTATTIVRERERGTIEQLIVT
                     PIRSWELIAGKLLPYVILGFVETFEVLLIGHWWFGVPIRGSAVLIIVTSGLFLMSSLG
                     IGLFASTMANTQQEAMMTVMMFNLPMIFLSGFFFPIAAMPRFLQIVSYAIPLRYYLII
                     IRSLLIKGVGLVAIQNEVIALVIFGSVIMGAAALRFRKRLD"
     gene            complement(25376..25993)
                     /locus_tag="PL000_04680"
     CDS             complement(25376..25993)
                     /locus_tag="PL000_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC88222.1"
                     /translation="MSAKSKDAPKKGEVTRLAIEDAAIELFMEHGYHATSMRQIADQA
                     GLALGGIYNHFASKEEIFEGIIIDKHPYKKILPLILEAKGDTAEEFLRNAMRIAITEL
                     SKEPYYIKLMMIEFVEFNGGHGAAMLKEVAPKVLPVFEQIVKIRKNLRVTNPAMLMRS
                     FFGLVVSYFITEMVVSNSIVGSLMPKNPMDVYVDVYLHGILKPES"
     gene            26094..27377
                     /locus_tag="PL000_04685"
     CDS             26094..27377
                     /locus_tag="PL000_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88223.1"
                     /translation="MLPFTTLEKQIRADLKYNIKVNLWDGALFGVALGFASFGTVLPL
                     FVASMTNSALLIGLVPAIHSVGWQLPQLFTASYVSRLRRYKRSVMMMTIHERVPFLGF
                     GIIALLLPMIGLKVGLVITFLLLIWQGLGGGFTANSWTSMISKIIPPENRGTFFGFQA
                     GLANLFISGSAVAAGYLLDYLDSPLDFAACFFIASIFFTLSWFALALTREPEDTEKII
                     PEEKTHFWDDSKKILKKDFNFNWFLVARFISQFATMGFSFYIIYALRRFNMDAITAGY
                     LTATLTIAQTIANASMGWLGDKWGHRSMLIIGAAAALASATLAWLATSIFWFYPIFLL
                     TGFANVSIWTIGMAMTVDFGTEAERPLYIGLSQTLTAPATILAPLLGGWIVDAAGFIP
                     TFSISIILSIAMIGILVFLVKDPRMHHSRKMKEVK"
     gene            27424..28614
                     /locus_tag="PL000_04690"
     CDS             27424..28614
                     /locus_tag="PL000_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HNC88224.1"
                     /translation="MSLIKEKVNQAIEILKEQQIDMWLTFVRETSGVRDPALDFLIGE
                     NDLTWPSALILTRKGEKIAIIGNLEKESLQRLDVFDEILGYDTAVSTLFKETITRLNP
                     DRIAVNTSRNNVHADGLTHAMYEFLREYLAGTPYADRLVSAEPVISAMRGRKTATEQA
                     RVKKAIEITEEIFAKTFDFIKVGMTEIEVGAYMHQLAKDYNVDLAWPAENCPAVNSGP
                     NSPVGHNGPTDIKIERGHLVHFDFGVKYEGYCSDIQRIAYVLREGETEAPIEIQRGFI
                     TIRTAIEKSREAMKPGVTGNVIDTISREIVADAGYPDYKYALGHQLGRVAHDGGALLG
                     PLWEKYGDSPNQKLEIGQIFTIEPGLPVNGYGYLGLEEDVVMTKSGAEYFHEPQKEIV
                     LLKG"
     gene            28801..30354
                     /locus_tag="PL000_04695"
     CDS             28801..30354
                     /locus_tag="PL000_04695"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC88225.1"
                     /translation="MTQEDPRILELRKMRAKALEGGGEERIAKQKAKGKMTARERVIA
                     LLDPGTFNEIEPFITHQGDELDLLKDKFYGDGVITGYGQINGRTVYLYSQDFTIYGGT
                     LSEMQSHKICRVMDLAVRNGVPFISLIDSGGARIQEGVRSMGGYAEIFRRNAQYSGVV
                     PQISVMLGPCAGGAAYSPALTDLVIMVEKQSFMFLTGPEVIKAVTGEMIDAESLGGSG
                     VHTSVSGVAHLSVQSEKAALEMARHFLSYLPSNNVENPPFTPTTDDLARMDEELNHMI
                     PLEATEPYVMHQVIEKIIDQGSFLEIQPDWARNAIVGLARIGGHSVGIVGQEPAIMAG
                     VIDIDAADKMTRFVRMCDCFNIPLVTFVDSPGFLPGIAQEHGGIIRHGAKLLYAYSEA
                     TVPKICIITRKAYGGAYVVMSSKYLGTDVTYAWPSAEIAVLGAEGAANILFKKQIETA
                     NDPTAERKKLANEYREKFNNPYYAASTGYVDDIIEPRESRPKLIATLSALRDKYAPAP
                     PRKHGNIPV"
     gene            30409..30774
                     /locus_tag="PL000_04700"
     CDS             30409..30774
                     /locus_tag="PL000_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HNC88226.1"
                     /translation="MTTDLLLSLQITALGMGLVFGAILLLWLMMALLTRFTAEKESAA
                     ETDSTAQTGASDSPEPAPVSTSGFKAQAAALAVALALAEQELSSAHPLLEPPTAIVSA
                     WQLGMRTSQRSEKGNFRRH"
     gene            30803..31264
                     /locus_tag="PL000_04705"
     CDS             30803..31264
                     /locus_tag="PL000_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNC88227.1"
                     /translation="MKYKVTVNNQVYEVEIENINTRPVIAHVDGERFEIMPENAGQAE
                     TGKDSSIPTEKKAFNSSPVTAAKPSPNPALSGNILTAPLPGTIVEVFVKPGEKVEAGQ
                     VVVIIEAMKMKNSIRSVYSGTVNEVFVSAGQSVAHKQALVKFADLGEASWM"
     gene            31305..32441
                     /locus_tag="PL000_04710"
     CDS             31305..32441
                     /locus_tag="PL000_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNC88228.1"
                     /translation="MDLTSLLPELLKGFTHFTLGNGVMIFAALILVYLAVFKEIEPVL
                     LLPIGFGCLLANIPLAGMTAAEGMSKVLYDAGIKTELFPLLIFIGVGAMIDFSPLLAQ
                     PRMVLLGAAGQFGIFGTLMLAIALGFPLNQAASIGVIGAIDGPTSIFVATKLAPELLA
                     PIAVAAYSYMSLIPIIQPPLMKLLTTKKERMIQMDYAPTPISKKTLIFFPVVLTIVVG
                     LLVPEATPLISMLMLGNLLKVSGVVDRLSNAAQNEIINIATLFLGLAIGSTMSAASFL
                     NLDTGKIMILGLFAFALDTVAGLLFGKLMAVVTGGKINPLIGAAGISAFPMAGRLAAK
                     TANDEDPNNFILMHAMGANTAGQLGSVIAGGILLSVVSKILGIG"
     gene            complement(32577..33038)
                     /locus_tag="PL000_04715"
     CDS             complement(32577..33038)
                     /locus_tag="PL000_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88229.1"
                     /translation="MNNTKTTAISGCVIWFLLISVIGSCVMPVFFMVGSFTSFSEYAI
                     KTTGGFLCPAGTTPKSYSYSSTSFDEFGNPENGTAYELHCVDASGTVVKSDPIFYAFL
                     WIGIWALIGAVVSGLLTFVFAVPGGMLVTKLLNKLKRPSTNITSSDYPKFD"
     gene            complement(33040..33423)
                     /locus_tag="PL000_04720"
     CDS             complement(33040..33423)
                     /locus_tag="PL000_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88230.1"
                     /translation="MKAMKFWWLIFVFPVVIALMVTSIALVPNNPLRKVAGMLVCPSG
                     SILKVTASDTTWYEDGFIREGTGYSFDCVNLSGAPVENNIDTVFTVLLLATILSPFVI
                     VLARVFAPQKQVKHESMYDGKMKVG"
     gene            33578..35044
                     /locus_tag="PL000_04725"
     CDS             33578..35044
                     /locus_tag="PL000_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC88231.1"
                     /translation="MTTIDLTVHKDRRARAIQRAKERNIIIPTYAQMKDPSKIPAKIK
                     EELTKIGLWDIHPRNLFRINWHNQPTASGGTFGGVNYLELPSSLTGVKARIIAVVGKW
                     FPTGAHKVGAAFSCLVPRLVTGQFDPTTQKAVWPSTGNYCRGGAYDSALLGCESIAIL
                     PEGMSKERFEWLAKVAGETIKTPGSESNVKEIFDKCKELATSGQDLMIFNQFEEFGNY
                     LWHYEVTGHAMEEVFKAVAGKHGRFRGIASATGSAGTIASGDYMKQLFPESKIVASEA
                     LQCPTLLENGFGAHRIEGIGDKHVPWVHNTKNTDIVTAIDDNAVVNISRLFNEENGRA
                     YLAEKGVPESVISNLDLLGFSGISNVLSCIKTAKYYEMDENDVMITMLTDSMELYRSR
                     LHEMHQEFGEYSEKDAAADFARYLHGQSTDNMLELTYPERRRVHNLKYYTWVEQQGKT
                     YEEIQNQWYQPDYWTSVQKQANEIDELIVEFNKEVGLV"
     gene            complement(35222..36289)
                     /locus_tag="PL000_04730"
     CDS             complement(35222..36289)
                     /locus_tag="PL000_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC88232.1"
                     /translation="MELLWPGFLYLLALIPLLVLVYIFVLRRRKPYAVRYSSLSLVRE
                     ALGKQSQVRRHLPFVLFLLAMSSLVVAMGRPVSTITVPASNATIILAIDVSRSMCSTD
                     ISPNRLEAAKTAAWDFIAAQESNVQIGLVAFAGYAVVIQEPTTERSLLETAIQNITTA
                     RRTAIGSGLLKSLEALSEIDDSMPSPFAGTEPEPMPAGQYAPYIVVLLTDGVNTTGPE
                     PVFAAEFAKTRGVRVYTIGFGTAQEDAPMNCGGFQGGGNPNDPNQFFFGGGGGGGGGG
                     GGGFRRGIDEETLKQVADMTGGEYFAASSTGELQNVFNNLPVQLMTVTETTEVSVFFA
                     AIGAVLILLALILSMLWHPIA"
     gene            complement(36421..37437)
                     /locus_tag="PL000_04735"
     CDS             complement(36421..37437)
                     /locus_tag="PL000_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC88233.1"
                     /translation="MSFLWPTLLFSLLAIPLLALLYLRMQNQRKQLAVRYGSLGLVQQ
                     TSGRAASSRRHLPAILFLIGLAILFFSLARPQMVASLPKVEGMVILAFDVSGSMTAAD
                     FEPTRMEAAKAVGRDFVQRQPETVKIGIVSFSDNGFSVQLPTNDKEAILASIDRLRPE
                     RGTSLANGIIISLNTIANVTGQPPILGMDVPQPDASSPAPTPAPVSSADDSAVIVLLT
                     DGENNMDPDPLEAAQFAADRGVRIHTIGVGSSAGVQLEVNGFSVFTQLDEATLRQIAE
                     ITDGNYYNAQTEEDLQTIYENIDPQLVIVEEKTEVTAIFAGASILILLVAGMLSLLWF
                     SHVP"
     gene            complement(37440..38339)
                     /locus_tag="PL000_04740"
     CDS             complement(37440..38339)
                     /locus_tag="PL000_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC88234.1"
                     /translation="MNTSTSTPTPERILHRLDWQVIRRLDGLLQGDYRSLFRGFGVDF
                     ADLREYQPEDDIRYIDWNVSARMNTPYVRQYMEDREVIAWFLLDLSPSVDFGTLQNQK
                     RNVLIDFVATLARLLTRHGNRVGAMMFGSAIQRTVPARSGRLQVLRIINDLLNQPRLP
                     HSSMTDLSALLKGGLHAIKKRSLVFVVSDFISTPGWERTLNLLSQHHEVIAIRLWDPR
                     EMDLPDIGPIVMEDAETGEQLYVDTHDKKFRERFRAAAQQRETALKDSFKRAGVDVLP
                     LSTEEDLIHAIARFVMMRRQHKR"
     gene            complement(38308..39318)
                     /locus_tag="PL000_04745"
     CDS             complement(38308..39318)
                     /locus_tag="PL000_04745"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC88235.1"
                     /translation="MEQTSNRENKLPMERVLYEVKRVIVGQDHLLERMIVALLARGHI
                     LVEGVPGLAKTMAIKTLADSIGGEFKRIQFTPDLVPADLIGTRIYNQKSGEFNTSLGP
                     VFTNLLLADEINRAPAKVQSALLEVMQEKQVTIGRETHKVPNPFLVLATQNPIETEGT
                     YALPEAQVDRFMLKVLVGYPSPTEEFVIVERMTGAWTEVQKVLTTDQLVELQKRTDQV
                     YVDPALIEYAVKMVNATRNPKEVGLGEVQRYIMFGASPRASINLILTAKALAFVRGRS
                     YVLPQDVLDMALDVIRHRLVLSYEALSDNISSDDILKKILERIPLPVVPLHEHFNVHA
                     NA"
     gene            complement(39426..40265)
                     /locus_tag="PL000_04750"
     CDS             complement(39426..40265)
                     /locus_tag="PL000_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC88236.1"
                     /translation="MNESSASKMDKWKGRMREVIARLRRGLPFASGVFGALLVLFFYN
                     LLVVKPNQLTVEDVNASVAMVMASATPAPSYSAQVYQAIRPSLILIQVEEKHQNAESD
                     FGLGSGVVVDSFGNILTSLHVVDGASVITVTFADGTQSEAAIVSEMPERDIAVLQAYD
                     TPLSVVPAVLGNPNSMRVGDEAFVVGNPFGLYSSMSSGVISGFDRVFQMEGTGLEIPG
                     MIQIDAAVNPGNSGGPLLNRNGHVIGIVTGIINPTDESFFVGIGFAVPITVAVGGMGS
                     PPY"
     gene            40474..41595
                     /locus_tag="PL000_04755"
     CDS             40474..41595
                     /locus_tag="PL000_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC88237.1"
                     /translation="MNGHCFISYSTADALDFARKLADELEGGHPYIRVWFDKRDLRVD
                     EDWDDQISSAIETCTCLIFVLSNDSTSQTSVCKEEWSWALKYKKPIILIRIQTNVQPP
                     FRLGERKIIDFSADFIIGLAKLRKQLAYLDSPEGILSELKHSLVDAERDLHRTKGNEE
                     NLIKSEISELKKQIEVQQKILDNPQALQKQIQKNVNAELEGEHQHLKSERSVNRIFLS
                     YRRADSADVVGRIYDRLIQVYKKEFVFKDVDSIPLGLDFREYLHRMVSISTVFIAVIG
                     KNWESVQDEQGNRRLNNPADYVRIEIASALSRNIPVIPVLVQNSSMPLVDDLPDDLKN
                     LCYRNGISVRPDPDFHNDMDRLISGLSKLEDINNKNFFK"
     gene            41666..42340
                     /locus_tag="PL000_04760"
     CDS             41666..42340
                     /locus_tag="PL000_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88238.1"
                     /translation="MLIYFIFPLIVVLIGLLIEYWVIQPLRKNGIPISFQTTVSSIIQ
                     RMTSFIKPDKINEESSPSEKKTSLNRILKSKLGIGITLIIFLLLLTASYFLIKANQKP
                     VYTYPTPIRYSIAIGDNLNKVIDIDQTHEYVFTSSSNKIVAITIRRIRTTDEAKRNLR
                     VNLYDENYVYLFEESILADTTDPAEKTIEFAPEIGVNYYLEIYLGSSPSNDYFGANYI
                     ISLEYK"
     gene            42440..42826
                     /locus_tag="PL000_04765"
     CDS             42440..42826
                     /locus_tag="PL000_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88239.1"
                     /translation="MIYHNASALHGIIASRQGEQGDEVAARGAFVRAIGQADEILSKT
                     AEYYSALDAKGLAICGLVLCRGAAPLRPYAQDIDNAIETFKKARRIAPHAGVVKSVLR
                     LFDELAKCDEKDVLKGTREVLEGKGT"
     gene            42829..43533
                     /locus_tag="PL000_04770"
     CDS             42829..43533
                     /locus_tag="PL000_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88240.1"
                     /translation="MFGLNAHEINSIVFTSLTSALITGVIVYLIQKSIENSFNKKMEE
                     FRASIQYTFFEQQTKFTRTFQKKIETFEVLHQKLNLLGQSMNSLANLIKNEITASSQK
                     KSEEYDKATETVSTSYLDFLHYYTENRLYLPDQSIETIEKISTKVGMLSMLIGLSGMI
                     STAPLESVTWATSWANREIKKIDLDIKSINPKKPNVPLFIDQIIKEMKYQIEALEKHY
                     KFTTMDDSTSISTRKF"
     gene            43704..>43992
                     /locus_tag="PL000_04775"
     CDS             43704..>43992
                     /locus_tag="PL000_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88241.1"
                     /translation="MNPIILHADHLVLMDSNNTVLQNGAVLIGADGRIQSVGQADAVM
                     AANPSVEVKRLENRLLMPGLVNTHMHSGLLRGTAEGLPVWEWLQQFIDPMHR"
BASE COUNT        11856 a        11239 c        10625 g        10272 t
ORIGIN      
        1 ctaggacgcg gccctttcaa ggccacgacc ggggttcgaa tccccgtggg ggcaccatag
       61 tgaacaaaaa acgctggata acccagcgtt ttttgttttt ttgcacttta aatggctgat
      121 tttagttttt tttccttgcg gggaaaaaag ggataaataa aagaaatccc aatgcaatca
      181 cgccctggcc gatcaaaacg cccgtggcct gccacggccc aaaataaggc actttcggaa
      241 aggattgtga acccatgcca aagacatcag ccagacccgc aaaaacagca atgacatagc
      301 cagtaccaac aagccttgtt ccaatatcgg cgaggatggt gcgttcctgc ccatgccaca
      361 aggttagcag actgatgtat ccacccaggc agatcaaagc aagacccact aaaaagacgg
      421 cgatttgcac aaagccaacg accgcactac gatcccaacc gaaccaatca ggtttggaac
      481 cgatcgtgaa aataaacaaa ccaacaaagg taagcgtcaa cccgacatac actcgtggtg
      541 cattctggtt ttgagcggaa tatggcgctg tatctacggg ggtggatgct gaaacaggtt
      601 tattcatact catgatggca aatcccttct taggcggttg agccagttac cgccaaggat
      661 attggagaca tcgacatcgt tataaccacg tgccagcaac aaagccgaaa gtttttgcag
      721 atcagcaact gtctcaattt cagggggaac agattgcaac ccaaagccgc cgtcaaaatc
      781 ggaaccaatg cctgcgtgaa gggaatcacc tgcgatctgg cagatatggt caatatgatt
      841 ggcaacatgg tcgagtgaaa cttcactccg ctcgcttttg ccagccaccc accccacctt
      901 gaggtatcca ttgaatggca ccactccaat aacaccgtcc cgttcaatga tcccttcaat
      961 gatgcggtct gaaaaatgac ggttcgaagg agagttcggc atcaatgaca gacagttggc
     1021 gtgcgtgcct acaatcggtc cacgataaat atccaaggct tccactgcag cttgttcatc
     1081 catgtgactg aggtcgagga taaagttata gtctgccatg gcggcgagca gtttacggcc
     1141 atctggagtc aatgggccag gttcattcca accaccacaa tagcgggtac caacccaggc
     1201 agggccgatg aggcgtacgc ccatctcgtg ccactcttcc aattcagaaa gggtacggat
     1261 tccttctgcg ccttccatga gcacaaccat gcccacgggg tgtccgctgc tggctgtggc
     1321 ggggaggctc tgccagtttt ccagaatcag gtccaagtca cggtttgatg cgatgagtcg
     1381 gaacttgtcg ggcattgcat cggccatccg atggtaggtc agcagttgtt cgcgataaag
     1441 ctgatgggct tcatcgtaag ttttgtatac ttgggctttg ttttcgcttt tgctccagcg
     1501 gataggggtg gcgtagaggg ttgaaaagat gaccgcaacc tggccatttt ggtattcggg
     1561 ccaaccaagc agagtatcat cattttgctt tacaacatca ctgcctgctt caatctggcg
     1621 cgtttccacg gctgaacgag tgtagtcacg tccatatttg agcatgttcc aggcaatatc
     1681 tgcatgtgca tcaactatga taggcatggg agattagtga agagcgcacc tttcggtgcg
     1741 ctctggatta tttttctact ctttgttctc ttcgtcgggg tcctggaacc cttgtgtaag
     1801 catcttgaag tctttgtcgg cgaaggcggc cgagtcaacc ttgcggaggc taagaccaat
     1861 gcggtgctgt tcaccgtcaa tgcggatgac gcgcaacgtc ttgacttcac cttccttaag
     1921 aacttccttg ggatgctcaa cacgattctc gctcaactca gagatgtgga tgagaccttc
     1981 aatatcgcct tcaagacggg cgaatgcacc aaacttggtc aggcgggtaa tgacaccttc
     2041 aacaagctgg ccgacgctga atttctcaac acgactcttc caggggtcat cctgtaaggc
     2101 gcggatggac agaccaatgc gtttcttgtc gcggtcaatg ttgataacct tgaccttgac
     2161 ttcctgcccg acttcgagaa tctccttggg atgctggagg cgatcccatg aaagctctga
     2221 gagatgtacc aagccatcgg cgccgctgac attgacgaat gcgccaaaat ctgcgaggct
     2281 tgtgactttg ccggtgtaca ccttgccttc ttccaactca ctaatgacac ggtccttcat
     2341 cgaggagcgg gtttcagtgt tggtggagcg ttcggaaaga ataaggcggc ggcgttcgcg
     2401 atccacttca ataatgcgga cggaaattgc ctgaccaacc atcttctgcc aacgctgttc
     2461 aggggtatca cctgaagact gcgcacggcg tacggcgctg atctgtgaag aaggtacaaa
     2521 accacgtaga tttccaacag cagcgatcaa accgcctttg ttgaaaccaa cgatcttggt
     2581 atcgatcacg gtttcatctg cgatcatctt ctcaacattt tcccaagaca tctcttcctg
     2641 tgcgcgcttg aacgataaaa cgacattgcc gttttgatct tcaggattga gaacgtaaac
     2701 gttcacttcc tgtccaacct tgagcgcggc gcgttcttca gctgtgagtt gttccaactc
     2761 acggccagct acaacgcctt cggattttgc accgacactg atgagaattt ggcttggtcc
     2821 aatgcttgcg atcatgcctt tgcgaatttc gccgacctgc ggcaactcga cactcaaaga
     2881 ttcagattcg agcagtgact ccattgtctg attgtggggt tggtctccct ggggatttac
     2941 agccccttgc ccagtttggg cttcattttg atccatcacg cgtgactcca attaaaactg
     3001 aaatatgatt tcgattatac aagtgattac agaacttgac aaccccgata tacgcattaa
     3061 aacaacagat tcttacccga aagaaaaaac tcgcttataa tcacgctctg gagaaaaaat
     3121 gcccgctatc ttcccattta cagccattgt tggacaggag cgcatgaaac gcgccctcat
     3181 tctcaatgcc gttgataccc gtatcggcgg cgtattgatt cgcggtgaac gtggtacggc
     3241 aaaatcaacc gcatcgcgtt cgcttgccgc tttgttgccg catgtcaaga tcgtacagga
     3301 ttgccgcttc ggttgcgacc cggataaatc atcgacatgg tgtaccgaat gtaaagaacg
     3361 ttatgccgcc agcaaaaaac tccccatcgc agaacgcgcc actccattta tcaatctgcc
     3421 tgtttctgcc acggaagacc gtgtggtcgg aacgctcgat atcgaaaaag cgattcaaaa
     3481 aggtgaacgc cattttgaac ctggtgtact tgcaggtgcc aatcgtggac ttctctacat
     3541 tgatgaagtc aacctgcttg atgaccatgt agtggatatt ctgctcgact ccgctgccat
     3601 gggtgtgaac accgttgaac gcgaaggcat ttcttttgcg caccctgccc gtttcattct
     3661 cgttgggacg atgaatcctg aagaaggaga tctccgcccc cagttgctgg atcgtttcgc
     3721 cttatccgtg gacattgtcg gtattcgtga tgctcgtgaa cgcgtgacca ttatggagcg
     3781 caatctttcc tacgaaaagg attctgaagc cttccgcaaa caatggcttc ccaaagaaga
     3841 agaactttcc agcaagatcg cccgtgcccg cgaactcgta gaccaggtca cccacaccag
     3901 ccgtgacttg ctctccatcg ctgccttgac cgcctccttg aacgtggatg gtcatcgtgc
     3961 cgaccttgtc attctcaaag ccgcccgcgc tgaagccgcc ttcgaaggcc gcaccaagat
     4021 caatgaccac gatattgctc ttgccgcaga actggcgctt ccccaccgca tcaaacgcac
     4081 gcccttccaa caagccgaat tgaccaccga acagttgcag gaacgcatcg aacaactggc
     4141 ggggcaatcc atgcctgaag attcggatga aaaatccgaa agcaaacagg atggcgaagg
     4201 cgaggaaaaa aaaacttaaa gcttgaagat gagccacagg aaggtcccca gcagggcaac
     4261 cctgaaccca tgcagcagca ggaagttttt gcaagttcca atgcgaattg gtgggaaggc
     4321 ggacaaaaag taaaagctgg tgagaccttc cagcctcgta aactcaatac cccactcgat
     4381 aaactaaccc gcaagcaagc tggccgccgt tcgctcacaa aaacggagcg aaaacatggg
     4441 cgctatatca aggcgcgacc cgcaggcgac aaactcaacg acatcgcctt cgatgccacc
     4501 ttccgtgctg ctgcaccatt ccaaaagcaa cgcaccgaag aacgcaagaa gctcgcattc
     4561 tccatcaaga aaagcgacct tcaacgcaag atccgtgtga agcgggttgc aaatttggtt
     4621 cttttcctag tggatgcatc ctggtcaatg gcagttgcgg aacgcatgaa tgcaaccaaa
     4681 ggcgccatcc tctcgttatt gaccgatgcc tatcaacgcc gcgaccgtgt aggcctgatc
     4741 gtctttcaaa aggatcgcgc caccctggtc ctctccccta cgaactccgt tcaactcgca
     4801 caacgcgccc tgatggatat ccccgtcggc gggaaaactc cgctctcggc tgggttgttc
     4861 atggcgcacg atgtactcac ccacgaaaag cacacccacc ccgatgtgga accgctcctc
     4921 atcgtgctga ccgatggtgc tggcaacgta tcccttggca ccttgccacc gcaggaggag
     4981 tctcataaat ttgccgaact catcgccaac gaaaagatac gctccgtcgt catcaacatg
     5041 gaacacgccg ccttcgatca gggacttgcg caagaactcg caaacaacct caaagcgccc
     5101 tgctatgcgc tcagtgaatt gaaagctgaa agcctgtaca acactgtcat tgatgaaatg
     5161 tcgaagccag cgaagaagta atcaacacac aacagacctc ttgtaaaatc tgaaagttta
     5221 accactgagc cacggagaca cagagaacca aattaaaaac tccgtggctc agtgtctttg
     5281 tggttcaaaa tggtatttca aagacattcg ttacagcata cgcaggatat caaataaata
     5341 agacacagaa acatagagga aatcaataaa ctccatgttt ttgtgtcttt gtgatttaaa
     5401 agtttttagt tgcctacgtg ttcaaaaaaa caactaaaca tccaaactgc ggttacgaat
     5461 caaaccccag cccgagtgca tccaagatct tcaaccatag atttctttgc ccgccattat
     5521 cgtcagcgcg attcaaagac catcgtgtga agttgacgat cggggaacga aatggctcag
     5581 gcgggaacgg aaacggtttc gatttcacca tttgcaattc ggtgcgttcg gtctttttgc
     5641 catccaacag atccaacatc acttgcgcgc caaagcgtga tgcgccaaca cccagacccg
     5701 tgtagcccat ggcataggcc aacttcccac cataagcctg accccaaaac gcagtgtagc
     5761 gtgaacaagt atcgatcact ccaccccagg cgtgtgtgaa acgcagaccg gtcaactgcg
     5821 ggaaggtttg gaaaaaatgc tccgccaaac gagcaaaagt ttcggggcgg ttttccaagt
     5881 gcggagctac tccgttgttc tggtaataaa tggcatcgta tccgccccac aaaatcctgc
     5941 catcggcagt cgtgcggtag taatggaatt gattcccatt atcgctcaca ccttcgcgtc
     6001 catgccagcc gatcgcatcg cgctgttcct ttgagagcgg ttcggtcatc agcacataat
     6061 cgtaaacagg aaccacataa aaagaaagcc gtttcagcaa aggcggaaac gcattcgtgg
     6121 caagcgccac acgtttggat tcgacctgtc cgtatgatgt tttgacgaga atcgaatcgc
     6181 gcttctcttc cagactcgta acctgtgtgc cctcaaacaa gcggacacca acctccaggc
     6241 acgctcgcct caaaccccaa accaatcgtg cagggttgac cattgccaca gacgaatcat
     6301 ggattgcagc aagataagtc ggtgaattaa cccgggcgcg gatctgctca cgatcgagga
     6361 attgaacatg ctctccatac tctgcagaat gttcggcttc ctcgcgcaac tcttcgatct
     6421 ggtattcttc attggcaata ttgatctcgc ctgaacgtat gaaatcacaa tcaatattaa
     6481 attgcttgat cgtggcttcg attccatcca gatttttatg ccccagctca atgatctttt
     6541 ttagctcttt gggccagcgg ttcaacccat tttgaaagga atgggtcagt gatgaggcga
     6601 cgaaaccgcc atttcgtccg ctggcgccga tgccagtttc ccctgcttcg agaaggacca
     6661 cttcacgaga tgggtcggct tgctttgcta gaagggcggt ccacaagcct gtgaagcctg
     6721 cgccgatgac aacaagatcg gctgtgatgc tttcggtcaa ggccgaagca ggctccggac
     6781 gtgccgggtc atccaaccag aacggcacgt gtttcacatc cgcaagagat ttcaaggtct
     6841 cagggcgcgg agtgttcgag aaaatcatta ggtctccatt ttcgcggcat ggccgcctcg
     6901 cagtgagtat gcaggccatg ctcatagatt tgttgtagtt tacgctgtag atggagagga
     6961 gtcaaatatc aaattatttc acaagggttt cttgcgggtc atcgcggtaa acaaaccgcc
     7021 taggaaaatt gcggccacgc caatggcggc gattcccatc atattttccc gtctttgttc
     7081 gggggtcagg gttggggttg gttctggctc tgcagtttta gcaggcacca tggtcggggt
     7141 ggggaatgtt tggggtgact gggcaagtgt cggtgcggtg gatgcgccgc cgggggcggg
     7201 ttgcgtcgcc cctttcagca ccagcagttt ttgtcctgtg cgcaaattcg tgtcggtcaa
     7261 tccatttaac tgctgaattt gcttaatggt ggtgccgtag gaaatcgcaa tgctccacaa
     7321 ggtttggccg tatttgactt cgtgatagac attgccatca ggcatggcag tgttgagaaa
     7381 gacgggagca acattttgcg gaagcaatcc attttcagcc gcaagggttg ccatgcttga
     7441 ataggcggcc tgggtcatgg ggatgcctgt gaggtaaaca atggcatcgt attgcatctg
     7501 tccgcttttg gtttgaagcg cagtctcaag tacaatgaca tacacatctt catcgggcat
     7561 ttttgcgatg cccacgccaa tatcgctgcg ctcaagagac agcattgtgt tttcgtgttc
     7621 gtcatcgctc agccacatgg ctacggcttc gtcggcattg tgggcgaatc cccagttttc
     7681 agaacggtag ccatcgagtg aaatatcacc cgaaagcgga tatccgagcg aaagcagcca
     7741 ttgtccaagc gtaatgcctt cgggccgcca atgaccttcc ctgcctgccg caatgccatc
     7801 tgcttccatc tgaccgattt gcatcaaaac agggtgtgca ttcaacggtg cgatgccgtg
     7861 tgaaaggcgc aggttgttga ctccttccat gatctcagca ggcacaggcg ggcggtcaga
     7921 agagggagtc ggtgtttcct cgctttgagc cgagggatgt gcgaatgcat ctgaaaccga
     7981 gagaaagcca agtgatagaa caaaggctgc aaagactgtg atcaaaaagg tttgattttt
     8041 catatgcgcg agtttacaga actcatggtc tccatgaagg ggcgtaccag tcaaaatcgg
     8101 tggaaaggcc cggggcttgc aatattttct gctccagtgt ggcaaggtca atgaggaaca
     8161 aatgcccgcc tgaaatggaa gtggtctcct cgaccaacag ggttctgcca tttggagaaa
     8221 aaagcaaacg accaatggtg gtgcccgtat aaaccagcga ctgggtcttt ccatctctgc
     8281 tgatcacatg cacttcggca atcggcgaac catcggcgga attgctgtaa tgattgaagg
     8341 cgatcagcga actatcaggc gaccacacca acgggtagat cgatccgcct gcaaaactgg
     8401 tgagggcaac ggcattggca ccatcaggct ccatcacctt gatgacatgg ccttgagtat
     8461 tgtaatcaat ttcgtcgtag gccagtaaac tggcatcggg tgaagcaacg aattgcgatt
     8521 tggactgctg ggtttcaaac atcggtttgg cggctccgtc actggcgcgc acgagaaaaa
     8581 tattccccgc attggagggc aatgccgagc gcatcaaaat atgatcggtg taccagccat
     8641 ccataatata aggccgtcct tcaacaggca ggttcagcga tacacttttc agttctgagc
     8701 catcacggcg cacgacatac acatcttcac cgcctaatcc atccagcgtg cggaatgcga
     8761 tccaggtgcc atcaggcgac cagaacggcg ggtcaatgta tgcgaactgc gcgatcgaag
     8821 tccacgtgtt ggcggcgggg tcaaagatcc atagtttcgt tggagtgcca ttggacgaat
     8881 cggaatacga aaaggcggca agttttccat cgggcgacca ggacaaagcg ttgatcgtaa
     8941 aattaaaagc aaaaggaact gtcacaattt gcatcggcgg acaattggcc gaatcaaata
     9001 cacagatgcc tgaaacgcgt gccaattgaa tggattgtcc tgcctgccgc ggttgaagga
     9061 agaaatacaa ttcacccgca tcttcgctgg acggggaacc tgatgctgtt acaacaggca
     9121 cagcctccgc ttcagcattt ggcaaaccag cctgttgcaa accagcagga ggcgtgaaca
     9181 aagtttcatc aaaggtcaca tcaaaagaga tgtgatccac aacccgctcc cctgccaact
     9241 gactcgaatt gccttttgtg aattcctgta attttagaag gatgcccgtt tgggtatcca
     9301 cccacatttt cagagaggga agtgaattgg catcataggt ccattccacg acgagggctt
     9361 cgcgacctgc gatactttcc gctgcaaggg gtttgaaaat cccgccgttt tgtgaaaaat
     9421 cggaagcaaa tgccaattgc gaaagcggag tacccaactg cgtccagatc gggtttgtat
     9481 tcacctgccc gggaaccaac ggaggaatat attgtccgac ccgaatgaaa tctggcacag
     9541 gagctatctg cacctgcgca agcgagaggt ttgcatcgta gagatttaca ccatcaatta
     9601 atttcaaccc atgttgggcg gggtcggtaa ttccattcac ctctactttg aatctgcttt
     9661 tgagcggctc caaccagact tgttcgcgga aggtttgtgt tgttccatca ggaagatagg
     9721 tggtaatggt tccatccatt tgcagggtgg tccattgtgc ggcactttcc aaaagcgccc
     9781 gctgaatttc ctcggaagcc gaatcaagtt tcaggtcggc gggagcgggg gttacttcaa
     9841 aaaaagaagg ctcggcaggg gcggaagtcg gtacagcacc cgagcaggca gccagcacaa
     9901 agatcaacgt acccaacaaa aatcgtttca tgtattctcc aaaacaaagg tgtgaccagt
     9961 tgacgactac ggaaaacaaa agttcccgcc gccttattgt gggcgacggg aacaattacg
    10021 aaagtggatg tttttagggt gtcggtgttg ctgagggcgg gatgggagta gccgtcacat
    10081 ccggtgtaag ggtaagggtt ggggtcggcg gagttggggt gcccggagtg gatgtagctg
    10141 tggcggctgg gacggtaaca gtcacagtgg aggtcggtga aggagttgtg gctgtggctg
    10201 agatggtggc ctgaagggtc ggtgaaactg taaaggtggg tgttggagaa gctgaggaag
    10261 agcctgaacc cgatgaagca ttgtagatga tcggggaaac ccacaacgca cggtcaccag
    10321 tggccgaacc agtggaaagg acggtaagaa tgaacttgag gtcctgccca gccagcgggg
    10381 tcagatcaat atcagcggtg taatactggc cttcataccg ttccacaaaa gcccaaaagg
    10441 tttggatgga tgaactgccc gatacggaat aatcgagacg gaaaaccacg tagcaggagg
    10501 tagctccgct ttcgcaattg acgacagcgc ggaacttgtc acctgatttc actttgtaag
    10561 aggggtaaat accctgaata tagccgttgt tgatattctg cgggaaggtg atcaaccccg
    10621 agcggttatc ggtggtgcca ttttccagtt tggatggatt ggtgatcaac acaaatccct
    10681 tggcatcatt atctgtaccg gggcagggca atgtaccagc gccgctatac caggtcgctg
    10741 cacaaacatt ggatacaaag tcatagctgg tgccagaaat gggcgtagct ggagttacag
    10801 gcgtgcccgg agtcaccggc gtagcaggtg tggatggggt gacaggcgtg ccagccacct
    10861 tgatctcaac ccagaaggat ttcgtaccat cagaaccgat tccaaacgga acgccagtgc
    10921 tgctcttgag tttccaatag ccgcgatagg tgccagcgtt ggcaggggca gtcagattga
    10981 tggtgatatc aacagtttgt ccaggcgcga cagtggtggg gataagcgca gagtcgggtc
    11041 cgcccatctt ttcgccagaa tcaaacatca ggctgtagtt ggtccaggtg caggtaccca
    11101 cgttcttgat acgccaggtc tttgcaaagg tggtgcccgg ctggaaggtc gtaccatccg
    11161 caacggttac atctttgatg aactgggcgc ggtcacaggc attcggcccg ggagtgggag
    11221 tctttgaagc cacggtcgtc ggcaatgtac cggtgactgt gggagtgttg gtcgcaacag
    11281 tgccgccagc gcgggcaatg acagggttgc cccacaacgc gcggtcaccc acctgcgaac
    11341 cataggcaga gatcaccagg atgaatttca catcctgccc agccaacgaa ctcaggtcaa
    11401 gattggcatt gtaggtcagg ccttcatact tttcgcggaa ggtccagaaa gtcttaacag
    11461 ggttcgagcc gatctggtaa tcgaggcgat atgccacata acaagaagta gcgccatact
    11521 cacaaccaat ggttgcctgg aagcggtcac cactctgcac tttgaaagca ggatagctcg
    11581 cctgaatata gccgttatta acattgttcg gaacgaagag cagcccagcc tgcgctgatt
    11641 cgattccatt ctcgaatttt ggcttgtcca gtttcagggc aaagccattg gcactgccgt
    11701 cggtaccggg gaaggtcaaa gtacccgcac cgctcgacca ggtggcagaa gcggcgttcg
    11761 aggtaaagtc atacccagtg cccgaagacg aagtcacatt gatctcaacc cagaacgatt
    11821 tgttcgcggt tgaaccaatg ccaaacacac caccctgtgt gctcttgaat ttatagtaac
    11881 caaagtaatg acccgccgca cttggcgcaa ccatatccac agtgatatcc acggtctgtc
    11941 cggggttcac agtggtcggc agggcagatg aagcaggcgc gcccatcttt tcaccgctat
    12001 cgaagatcaa agaaacatcg ccggcattcc acgcacaagt gccgatattc tttaatcgcc
    12061 aggtcttctt aaaggctgtt cccggggcaa aatttgtccc atcaggaatg gtcacgtcag
    12121 cgatgaactg cgcccaataa caagctgcag cctgcgctgg ctgtgttgcc gcagagggca
    12181 caactgaaaa catgaacacg agcaaaagca acacagcagc aaatttagat gtgaatttca
    12241 tttcttcctc catgtaaagc aaaagcttta ataatgacgg taattttctt gaaaggttcc
    12301 atttgactat acgccatccc tagtataact ttactcataa gaatgttcaa tatattaaca
    12361 aagcaatttc ataaccattt ttcctaccgc cgcaaccacc cagtacacta taatctcaat
    12421 catgccacgc acaatcctcc atttggacct tgacgcattc tactgttccg tggaagaaac
    12481 gcagaacccc gcactacgcg gcaagccctt tgctgtgggg ggcaaacccg atgaacgagg
    12541 cgtggtggca tcgtgttcat atgcagcacg cgctctcggc atccgcagcg ccatgcccat
    12601 gtcacgtgca gttcgattgt gcggtaatct catcatcata ccagggcggc ataaattgta
    12661 cggagaatat tccgaaaaag tgatggacaa attgcggaac ctgactccgc ttttggaaca
    12721 aatttccatt gacgaagcct ttctcgatat ttcagatatt cgcgaatccc ccgcccggat
    12781 cgcgctggac ctgcaagcgg gtatcaaaaa cgaactgcat ttacccagct cgatcggaat
    12841 cgcgtcgaac aaacttgtag caaagatcgc caccgaggtc ggtaaaaaat cgagcaaaaa
    12901 aaagaacgaa ccgccatttg gtttgaccat tgttcccgct ggagaagaag ccgcctttct
    12961 cgctcccctg cccgcagaca tgctttgggg tgtcggcccc aaaacatccg cacggctgac
    13021 cgagcttggc atccgcacca ttggcgatat tgccaattgg cctgaaaaag accttgtcca
    13081 tctcttcggt gaaaatggcc gtgacctctg gcgtcatgca catggtattg ataatcgccc
    13141 catcgtcacc gaatacgaaa ccaaatccat cagccaggaa accaccttca atgtggacgt
    13201 gcgtgacgac aaaacccttg aaaagaccct gcgcgaacaa gccaaagaag tcgcccgcca
    13261 actccgcaaa aatgaccttg ctgggaaaac cgtcaaaatc aaaatccgct ggcctgattt
    13321 caccacactc acaagacagg tcaccctgcc cactgcaact gacagtgaag atgacattat
    13381 caaagccgcg ctgaaactat tgcaggcgat tcgcaaaccc aatcaagccg tgcgcctgct
    13441 cggagtcggc gtcagcggaa tcggtgcccc agtacgccaa ctcagtttgt gggacgcagg
    13501 aagcgagaaa tcccgcaaat tgcaggaagt ggtcgatcag ctccaggaaa aatatggaag
    13561 gaacgtcatc caccgcggag aataagcgga aaatccaaaa caatcagcaa agcggatata
    13621 tgtcataatt tgacatatat ccgctttata ctttttatgc aggaacaagc catgtctaat
    13681 gttgccaccc gtttgatcac gctcattctc actttgcaaa gccagccaaa ccagaaagcc
    13741 agcgaccttg caaacaagct cggtatttcg gtgcgcacct tgcaccgcta cttcagcatg
    13801 cttgatgaga tgggcattcc tgtctatgcc gagcgtggac cttatggcgg tttttcgctc
    13861 gtccgcggat ataaacttcc gccgctgatc ttttctgttg aagaagctgt ggcagtttat
    13921 ttgggaacca acctgatcag cgaagcctgg gggaagttgt ataaagattc agcgcaaagt
    13981 gcaatggcaa agatcgaaaa catactgccg aacgaacaac gggatgagat ccactgggca
    14041 cggcgttctc ttgtaacgac cagtttgtat cgcgccgacc tcgccagcct ctcacccatc
    14101 atggaaaaac ttcgttccgc cgcacgcgag ctgaggcagg tcaatataac ttaccagggg
    14161 caaaccaatg caaaagccga aaaaagaaga attgacccat tcgcattggt gtttcgtgca
    14221 ggctggtggt atttggttgg atattgtcac ctgcgcaatg cgccacgcac tttccgcgtg
    14281 gatcgaatcc agaaattgag cgttctggaa aaatcatttg aggcacctgc cgatttcaat
    14341 atccatgcat atttagaaaa tgagttcaag aaccagccaa gtattcgcgc aaaactgcga
    14401 tttattcctg aagcagctca tatggtgaaa gcgaatccat ccatgtggga aacggttact
    14461 gagaattcgg atggcagtat ggatgtaacc ctgaccgcac cagatttgcc gtggctggct
    14521 tccatggcgc tgagtttcgc ccattggaca agggttctgg agcctcaaga actgcaggta
    14581 atgattcgag aatgggcgca ggcaaccgca gatttataca gcgaaatcac ccgataggag
    14641 gaatcatgtc aaaaccagac tccaaagatc aaatgctcaa agccgcacaa gaagagagag
    14701 atgcattgga agcacttctc aatacgctca ccacagagca aatgacccag cccaacatcg
    14761 ttggtgagtg ggcgatcaaa gatgttttga gtcatcttat ggaatgggaa aaaatggtca
    14821 tgtcctggta tgtgaatggc ttgaaaggca aacccacatt tgtgccatcc agagaattca
    14881 actgggggca acttccgcaa ctcaaccatg ccatctacct aaagcatcgc gataagaatc
    14941 ttgccgatgt tcaaaaggaa ttcaaagcct cctacaagaa aatcatcaaa accattgaag
    15001 gcattcctga aaaggagctt ttcacacgaa aaatctacgc atggacgaaa aacaatctac
    15061 ttgcagcata cttcatctct tgcaccagca gccattaccg ctgggcacgg accgtgatcc
    15121 gcaaagcaac gaaaagctaa taaaaggaga aaccatgaaa actctcgatt tgaagaaaca
    15181 attcaaatat ctgtatcaac cttcggcaaa aaagatcgag gctgtgcagg tgccgaatct
    15241 gcaatttgca atgatcgatg gcgccattga aaaaggctct gaacctggaa aatcgcccac
    15301 attcgcagaa gcgactcagg ccctgtacag cctgtcttac acgctcaaat tcatgctaaa
    15361 aaagcgcaag acaaacgctg ttgattaccc cgtcatggca ctggaaggat tgtggggtgt
    15421 ggaagatgga tttttcgata tcgccataaa ggataactgg acctacacac tgatgatcat
    15481 gcagcctgac atcatcacat ccgaggattt cgaggaagct cgtgaacagg ttcgcaaaaa
    15541 gaaaggcgat agtgaaatgc tctccaaaat acggctcgct cacttcgagg aaggcctgtg
    15601 tgtacaaacc atgcatatcg gcccttacgc caccgaacct gccaccatcg atagaatgaa
    15661 agaatacatg gctgccaata agttgaagga caatgtcggc ccaaatggca tgcatcacga
    15721 gatttacttg agcgatccac ggaaagcgtc tcctgacaaa atgaaaaccg tattaaggca
    15781 tccagtaatt gcgctataat ttcgtttgca aaaaacttca caaaagaagt ggaaacgaat
    15841 tgaaatgaaa aacagattcg cgttatttat actcattgca gttttgatcg gcagctgcgc
    15901 ccctacacca tcgaacactg caactgcttc actaccatca tccactgtaa ttgcagattc
    15961 ccctactgca acgatcgcgc cctcggcaac tccgacccca gtgccaataa cccttcctgt
    16021 tgatcgtttc aacccgatcc ctgaacttaa cccaattacg acagaaaata tcgacgctct
    16081 ggaagaagtg gctttttttg acaattctcc catatccaca cacattacag aagacaaaag
    16141 caggttgacc gtctggtttg aatctggctt acaaactttt gatatgccaa cactgacacc
    16201 atatcccttt atcaggatga atatgtccat ttttagttct aatgatgcga tctctgaaga
    16261 tggacggctt tttgccaggg catacccggg agtcaaagcc gaattctgga cgcccaccat
    16321 ttttatctgg gatacagaaa caggggaaga ggtatgcaaa atcaaactat cagagcatcg
    16381 agggaatttt ttctacacaa attttcactt ggaacagaat acattttcaa ttcatggaga
    16441 cctgggtccc aatggaaagc ataaagtcat catctacggg ctgaatactt gtcaaaggat
    16501 ctttgaccgg gattctgatt cagccatgtc tgcgatctca ccagatggca aatatgctgc
    16561 agttactcaa aagggacagg ttgttatcta cgacacgagc accaaaagcc ataacgccat
    16621 tggagaaaca aggggagccc agggagtggc attcttgcct gatagcggag aagttgccat
    16681 ctcctactcc gggcaaactt ttctatatga catgaccagc ggagaggtga atgggttctt
    16741 cgatgcaggt atatcgaatt tttattcaac aatttccgtt ttagatcacg gtgaatgggt
    16801 tgttatctca gcttccgaaa aaaattactt ctggaattca tctgaaaaac aggtgtattc
    16861 cgtacaggaa ttcatagggc gcgattttaa attcagcaac ggcatcctga ccacctacaa
    16921 aaccgttttc aacttaagaa ccaaggcaaa aatcagcatc gaaaattacg gacctcaggc
    16981 ttctacggcg ctttcatccg atcagagcta ccttgccgtc agttcaaata ttccaccctt
    17041 tgtaaccgat atttacgaga ccgctacagg caaacagatc aactcaatta aaggggtata
    17101 tggtccgatc accgtaggct ccgattcatt tatagcaagc agccacgaag ttgggaaggt
    17161 tttcaacttt attaccggag aatccgatca agaccttgac cacagctacc tgagcggctt
    17221 ccttttggat gaaaaggaaa tcgtgacttg ggatgcacta ggcaaaattt ccctgctaga
    17281 cataaaaaca ggcaaggctg tgacagcggc aaacctgccc gtctttccct caagtttctt
    17341 aaattacaca cgatacaaaa atgcaacgcc attatgggat gaactatcac agtcagattt
    17401 tgccagcctg ccgccttcaa acaccatggt ttcaaacgat cggaccatag ggatccagga
    17461 aaataatgga cagctacaga ttttcgccct ttcagatggg aatttcatcc cagcaactga
    17521 cgatccgatt gctgcttaca atctgacaaa ccttggtttt caattcaagt tcagcccgga
    17581 cgatacgatc atagcggggg caaattacaa ctcattaact ttgtgggatg gccaaaccgg
    17641 agatcagttg gaaagaatcg gacttcccaa tggcgccgtc acggacatac ggttttcagc
    17701 cgacggcaca aagatcgtca tcagcaagaa cgatgggccg cctcaaataa atgcatttgg
    17761 aaatctgtcc ttgatcattt ttgatgttct tgagaaaaaa atcgttcagg attacaaatt
    17821 aaatcagcag ctaaataaaa caggctgcaa cacaacatta ccctttgcat tgacatcaga
    17881 cggaggccgc gtcgtcacat tgacccagga ttgcaagata ggagtttacg acagtctcac
    17941 gtttgaagag ctttcatcct tcgggtcacc atattcagat tccaaccctg ctttttcaat
    18001 ctcgccggat aacaacctgc tggttgtagt tgacagaaaa aacattgaaa tttgggatat
    18061 aaaaaccgaa aaaagaataa gaaagatgga cattcctgaa cttgcaggaa catttgattt
    18121 ctatgctcac aaggctgctt ttacatctga tggtaagatg atcattgtca attcaacctt
    18181 ttggagccaa agttctgtaa caacattttg ggcagttcca agcatacaat aatattttcc
    18241 cttaaaattt gaaaaaggaa acaaacagcg ccatgaactg tttttaccac ccagatcaac
    18301 ctgctgtcgg cttgtgtaaa tactgccagc gcggactttg cctcagctgt gccactgttg
    18361 tagatgatgt actagcctgc aaagaccgtc atgaagacga agtccatcaa ctggaacaac
    18421 tgacagcacg gaacttattc cagtccaaac gtgtagcgtc aggctatatg cgaaatgcta
    18481 ttttttatgg cctggttggg gctgccttta caggttttgg gctatggcaa ttgaaatggt
    18541 tgggactgca agctgttgtt ttcataatgc tgggtgtatt cctgttctat gcggcaggtg
    18601 caaactatct cgaagcgaga aagcacaaat aaaatgggaa cctggatctg tcacctccgt
    18661 atcgccgaaa aactactaac tcacttccca gaactcgaca aaaccctctt tacctttggt
    18721 aacctcgccc cagattcggg catccccaac gaaacgtgga cggaatttga cccgcccaaa
    18781 gaagtgacac atttcctgca aaaaggcgaa ggcgaagacc tgattcgcga tttggtatat
    18841 tatcgtgaat atcttgagaa tcttcatttg catgatgatc cacaaaaata tagtttccgt
    18901 ctcgggtatt ttacccacct catctgcgat atcctctgga tgaaatacat cggctcaaca
    18961 acaaagattg cattccacga attatttgaa caggataaac aaaaggctgt cgatcttgtc
    19021 aaaaatgact ggtacggact cgatcaactc tacaatcgtg cgcaccccga aaacctgttc
    19081 tggcaggtca tttacaaaac accaaacccg ccatcctacc tgccattcgt tcaggaaaaa
    19141 gccctgcacc aacagtacgc gcacatccgt aaattttacg gcgaaccaga ctctgaatgg
    19201 tttattgaac gcccctaccc ctatctaagc gagtcaagca tgacgcgtgt tgttgaagac
    19261 actgttcaag ccactcttat tattttggaa aaaatcaagt acactgacat caataaaact
    19321 caaagttcaa ctgaaatcct cccacaggag tttatgcgtc cctactcaac acccctctaa
    19381 attatcgttt tcttaaatcc attttacagg tacatgaacg aattcgagaa ttttttcaac
    19441 ctctccaccg atcccatgac cattgcagat gcaaatggga aaattattca tgcgaacagc
    19501 gtctacacag ccattacagg gtggagcgtg gacgaactga aagcaacctc ttattgggat
    19561 ctgcttcatc ccgatgatca cccaaaggca cgcgccgcat tacaaactgt caaagctggc
    19621 caccctgttt tcgccctgga atacagattc ctctgcaaag acggcacata taaaaccttt
    19681 atcgcaaacc tcagtggaaa taaaaaaagc gaacatcttt acgccatcag caggctaaag
    19741 gaaggacaga tcgttccata tatcatggtt gcaaatgccg caccggtagc tgtgatcatt
    19801 atcaacacca gcggcacgat cattcatgcc aacgatctgg caaccacctt gtttggatac
    19861 gaagcggggg aattacagga aaaatccatt gaagatctaa tacccatgcg attaagaaaa
    19921 aaacatcagg cccaccgcgg agaatatcaa tccaacccgg tcaaccgccc aatgggttca
    19981 cactacaatc tgaagggttt gcaaaaaaac ggactggaaa ttcgggtgga tattgcattg
    20041 aaccccctgt acgagcagaa cgaactatat gttgcctgct ccattctcga tatcaccgaa
    20101 aagatcacca ccgccgaact cgccctaaac cttgaaaggg aaaacctgcg cctcgcccgc
    20161 cttgcccaac gcgatccgct cacgcaggcc tacaaccgcc gcaccatgga agaaatgttt
    20221 ccaaagatcg cggaagaatg caaaaagcaa aacaggagtg tctctgcaat tatgctggat
    20281 ttggataatt tcaaaacatt caacgacagc tacgggcatc aaacaggtga ccaactgcta
    20341 aaagagcttg cctacatcgc ccaaaaccac ataagggaaa atgatatcct tgtaagatac
    20401 ggcggcgaag aattcttcat catcatgcca aattgcaccc cgcatcaggc cagcgaagtg
    20461 gcggaacgga ttcgatcttc gctggaaaaa gacatgacct caggccacaa aatcacagcc
    20521 agcctcggga tcagcaccta tggtttttca aacggccccg gcgagcctct ggatgaaatt
    20581 ttggaaaaac tcattgaaca atccgatcag gcactatatc aggccaagga caagggcaaa
    20641 aaccgcgtac aacatttttc ctctatttaa tttgcaactc tttttatttg cctgcgtaaa
    20701 gcaagtagac aaacgaaagg agtaaaaatg aacgaagtca aaaccctcac ccgaagcaaa
    20761 tccaaccgca tgatcgcggg cgtgtgcgca ggccttgcag attatctcaa catggacccc
    20821 accgttgtgc gtttattatt tgtgattggt ttcttcacgt tgaacggcgg catgctggtg
    20881 gcctatttga tcatggccat cgtcaccccc gagcaataaa acaacaggga cacggcatgc
    20941 cgtgtccctg tgattatcga gccagtgaga ggaagacatc ctccagagtt gcctcgcctc
    21001 tttcgatttt ttccacccgc aggctttttg cacttaacag gctttgcatc gcctcttttt
    21061 cgacctgtga gctgatcacc cgtaaatgat cacccgccaa cccgacccgt tccacaccct
    21121 gaacggaaga aagtatctcc aatccctttt caagcgggta agcatacact tcccacacat
    21181 ctcctgtaat gaccgaatgt ttcagcgcgg tcggtgtatc catcgccagt aattttccat
    21241 cgcgcatcac ccccacccgt tcgcaatatt cagcttcatc catgtaatgc gtggtgacga
    21301 ggatcgtcac accgctttcc gccaattcat agatcaaatc ccaaaacgca cgacgggcag
    21361 tggggtctac accagaagtg ggttcatcaa ggaacaacaa tttcggctcg tggaccagcg
    21421 cgatgccaag tgacaaccgc tgccgccatc ctgtagacag gtctctggtg agggtatgtt
    21481 catgcccatt caggccgacc agttcaaggg tccgttggat gcgggttttg tcggtgatcc
    21541 cataaacgcc gccgtaaaaa gtcaggtttt ccagagtcgt caagtcatca tagatcgcga
    21601 atttttgcga catataccca acacgcatac ggatttcttc gctttccttg aagacatcat
    21661 aaccaaggac agtggccttt ccgtcgctgg gtttgagcaa acccaataac atgcgaatgg
    21721 tggtggtctt tcccgagccg ttcgggccaa gatacccgac cacctcaccc gcctgcactt
    21781 caaagttgat gtgatccacg gcaatgaaat cgccgaagcg acgggtgagg ttttgaacgg
    21841 tgattacagc ctcagtcatt gctcacctcc gataaagcaa taaatacatc ttccaatgca
    21901 ggtggaatga tacgcaggtc ggtcagtgtg ccgccagctg ctttgatctc ttcaggcagg
    21961 cgtttcataa tgaggtcagc ctgcccttcc aacacacgaa catgtaattt gtcgccgaaa
    22021 gcagccacat cctccacacc agaatcttga tgggcaacat gccgcagtgt gttgatgggg
    22081 ttgccgcgca attcaagaat gcggccatta agccgtgacc gcaaactgga cggggtatct
    22141 tcagcgatga tttttccttt tttcataaac ccaacccgat gacagcgtga agcttcatcc
    22201 atgtaagggg tggagatgat gacggacacc ccttcagatt gcaaagtttc agactgcgaa
    22261 ctgacgggct gggaaaccag cttgatcacc aattgccaaa agtcctgacg ggtaacggga
    22321 tccacccctg tggtgggttc atcaagcaaa agcaatcgcg gacgtgtgac aagcgcagaa
    22381 gccagcccaa gtttctgctt cataccgccc gagagctgcc ctgcgcggcg gtctttgaat
    22441 ttttcgaggc cgacgaattc aaggatctcc attgaacgcg gcaaccattc tgtagtggaa
    22501 agcccacgga tctcggcaaa gaagcggatg ttctcaagaa cggtaaggtc ttcgtacatg
    22561 gaaaaacgct gcgaaagata accgatcgtg gagcgggctt gttcagcctg cttgagtaca
    22621 tcgtatccgc aaatggtgat ctctccctgg ctgggcaaca atgcaccaac aagcagacgc
    22681 atggtggtgg tcttccccgc gccatctgaa ccgacaaggc catagatctc accaggtttg
    22741 atcttcaaat caaccccatc cactgcgtga acactgccaa agtgcctcac caatccattt
    22801 gcctgaacca aataatcatt cataaaaatc tgcctttaat ttattttgca catgtcttgc
    22861 gggtacatcc tgacgccaga atttatttga aaaccacatc ggcgggcatg ccgatcttca
    22921 acttgccatc agggtcagat acctgtaatt tgatggcgta aaaagtggag ctgcgtcctt
    22981 ccacggtttg gacattgcga ggggtgaact ccgcctgatc ggcaatgtga acaacagtgg
    23041 cagtgaaggt aagcccgggg aaagaatcca cgctgacttc agccgtttgc ccaaggctga
    23101 cctgcccata gcgatcttca gggacataaa cggtgatggt caggttggtg agatctgcca
    23161 tcgtcagagc aactgctcca ggctgcacaa attcaccagg ctccacattg cgggagagga
    23221 tgactccgct catgaccgca taaacatcca attttgcaat ttgcgcatca agcaggtcga
    23281 ggttggcttg cgcttgatct acggcggtct gggcctgatc gagcgtgttc ttagctgaaa
    23341 tcacagcagg agactccgtg ccagtttgca gggagagcag gcgggtgtag gccgcatcat
    23401 aacgctgctg ggcaatcacc acctgtccac gggcatattt gatgtcatct tctgcttcag
    23461 tattggtgag ggcattgaag tcatcctgtg cgcgttgcag ttcatcaagg acatcgttgt
    23521 agtaatcgta ggcggcatcc tggacggcac cagcttcaac agcattttca gcctgcacct
    23581 tgaccttatc tgcaatgagg aaggcggcgc gtgcctcaga gagtttcttc tcggcatcaa
    23641 gatactgtgc attgcccaga tcagagatca ctttttcaag attggaattc gcttcatcga
    23701 gagcgatctt tgcagcatcc acttcggttt gagcagcggc gagttggtct gtctggtcaa
    23761 taaaccataa aggttgattg aattcatcag gtgcagaaac tcgcaggtcc tttgcgcgtt
    23821 gggcatcttg cgcagagaga gcggcctgca gggtttggtc aaatttggtt tgggcggttg
    23881 tcagggcggc tttggcagaa tcaagcgcgg aggcggccac tgcccgttgg gcagtcagca
    23941 ggctggggtc gaggtgcaga agcggatcgt tcgcatttac caatgcgcct tcggccacat
    24001 ccactccggt gacttttcca gcaagttcgg gtgaaacgtt cacaatgaca gcttcaatgg
    24061 aacctgatgc ggtaagttcg ccgtttccat tggtgttaag tgtgaggaat gcaagataac
    24121 cgatggcacc aagtacaaca agagccagga cgatacgaac gggtacggga attgatttgt
    24181 gattcatggt ttaactccaa tgtttttaat aaaaagactg attatgagat cctctacatt
    24241 aatcgaggcg tttgcggaag cggagtgcgg cagcacccat gatgactgag ccaaatatga
    24301 ccagtgcaat tacttcgttt tgaatggcca ccaatccgac tccctttatc aagagtgagc
    24361 gaatgatgat gaggtaataa cgaagcggta tggcatagga aacgatctgc aaaaatctcg
    24421 gcatggccgc aatgggaaaa aagaaaccag aaaggaagat catgggcaga ttgaacatca
    24481 tcacggtcat catggcttcc tgttgagtgt tggccatggt cgaggcaaac aacccaattc
    24541 caagactcga catgaggaac aacccggatg tgacgatgat cagcacagcg cttccacgga
    24601 tgggcacgcc aaaccaccaa tgcccgatca acaaaacctc aaaggtttcg acaaaaccca
    24661 aaataacgta aggcagcaat ttaccggcaa tcagctccca cgaacggatg ggggttacga
    24721 tcaactgttc aatggtgccg cgttcgcgtt cgcgcacgat ggtggtggcg gtaaggatgg
    24781 aagtgatgaa ggaaaggatc atgccgatca caccgggaat gttaaagtag gctccatcca
    24841 ggtctgggtt gtaccaaacc tgcgtgcgca cttccaaagg tgcggagggt gctccacgcc
    24901 cgctttgcag ggtttgttca ctgaggatct tcgtggagta ggcctgccca acaagccgag
    24961 ccgtggaaag tgcagtggcg ccaacactgg catctgaacc atccagcacc atgagcactt
    25021 gtgcgtttcc ttcaattaaa cggcggtcgt aatcggctgg gatgaccagt acaacccgtg
    25081 tatcacccga ctcgatcaat gcctgatatt catccgttga gccgactgaa tattcaagga
    25141 tgaagtaatc ggcagcacga aaggcgtcca gcaaggcacg ggattgcgtg gttttgcttt
    25201 ggtcccaaac cgccatggaa atatttttta cgtcggtggt tgccgcatag cccagcagga
    25261 acaactccat aatgggcatg atgatgatca ttgccagggt acgcgggtcg cgaaggattt
    25321 gcaaaaactc cttgcgaata atggaagaga tgcgggaatt gagcataaag gtttcctagc
    25381 tttctggttt caagataccg tgtaaataga catcaacata cacatccatg gggtttttgg
    25441 gcatgaggct gcccacgatc gaattggaga caaccatctc tgtaataaag taggagacca
    25501 ccaacccaaa aaaggaacgc atcagcatgg cagggttggt cacacgcagg tttttcctga
    25561 tcttcacgat ctgttcgaac acaggcagca ccttgggagc aacttccttc aacatggccg
    25621 ccccatgccc cccattgaat tcaacaaact cgatcatcat caacttgatg tagtacggct
    25681 ccttgctgag ttcggtgatc gcaatccgca tggcgttgcg caggaattcc tcggcggtat
    25741 ccccttttgc ctccaggatc aacggcaata tctttttata aggatgtttg tcgataatga
    25801 tgccttcgaa aatttcctct ttactggcaa agtggttgta aattccacca agtgccagcc
    25861 cggcctgatc ggcaatctga cgcatggatg tggcgtgata gccatgctcc atgaataact
    25921 caatggcggc atcttcaatg gcaaggcggg tgacctcgcc ttttttaggt gcatcttttg
    25981 atttcgctga catggcttcc tttcaagacc aaaaatgaac gaacgttcgt ttttatttta
    26041 tgcaaaagta gaaatctgtc aataggcaaa ttgcatcggg taaaatcatc tacatgcttc
    26101 cctttaccac acttgaaaaa cagattcgtg cagacctgaa atacaacatc aaggtcaatc
    26161 tttgggatgg cgcattattc ggtgttgcac ttggctttgc ctcctttgga accgtgctgc
    26221 cactcttcgt cgcatccatg accaactccg ccctgcttat tggtcttgtg cctgccattc
    26281 attcggttgg ctggcaattg ccgcagcttt ttaccgccag ttatgtttcg cgcttgaggc
    26341 gctacaaacg cagtgtaatg atgatgacca tccatgaacg ggttccattt ttagggtttg
    26401 gaatcatcgc gctcctgctg cccatgattg ggttgaaggt cgggcttgtc atcacttttc
    26461 ttttgctcat ctggcagggg cttggcggcg gattcactgc caactcgtgg accagcatga
    26521 tctcgaagat cattccgcct gaaaaccgcg gcaccttctt tggatttcaa gcagggctcg
    26581 caaacctctt catcagcgga tcagccgtgg cggcaggtta tcttttagac tacctcgatt
    26641 cacccctgga ctttgcggcc tgcttcttca tcgcaagcat ctttttcacc ttgtcatggt
    26701 tcgcacttgc cctcacacga gaacccgaag ataccgaaaa gatcatccct gaagaaaaga
    26761 cccacttctg ggacgattcg aaaaaaatcc tgaaaaaaga tttcaatttc aactggttcc
    26821 tcgttgcaag attcatttcc caatttgcaa cgatgggatt ttccttctac atcatttacg
    26881 ccctccgccg cttcaacatg gatgccatca cagcaggata cctcactgcc acgctcacca
    26941 tcgcccaaac cattgccaat gccagcatgg gctggctcgg tgacaaatgg ggtcaccgtt
    27001 ccatgctcat cattggggct gcggctgcgt tggcaagtgc cacccttgca tggctggcaa
    27061 catccatttt ctggttctat cccatcttcc ttctcacagg attcgccaac gtatccatct
    27121 ggaccattgg aatggcgatg accgtggatt ttggcaccga agccgaaaga ccgttgtaca
    27181 tcggcctttc gcaaaccctc actgccccag ccaccattct cgctcccctg ctcggcggtt
    27241 ggatcgtgga tgcggcaggc ttcatcccga cgttttcaat ttccatcatt ctatccatcg
    27301 ccatgatcgg aattctggtc ttccttgtga aagacccgcg catgcatcac tcaagaaaga
    27361 tgaaggaagt gaaatagttt tctttcatcc catatcaatt ttcaccattt tacgggagcc
    27421 tccatgtcac tcataaaaga aaaagtaaat caagccattg aaatcctcaa agaacaacag
    27481 atcgatatgt ggttgacctt cgtccgcgag accagcggtg tacgcgaccc tgcccttgat
    27541 ttcctcatcg gcgaaaacga cctcacctgg ccttcggcgt tgatcctcac tcgcaaaggc
    27601 gaaaagatcg ccatcattgg caacctcgaa aaagaatctc tgcaaaggct cgatgtcttt
    27661 gacgagattc tcggatacga caccgccgtc agcacattgt tcaaagagac gatcacccgc
    27721 ctcaaccctg accgtattgc ggttaacacc tcccgaaaca acgtccacgc agacggattg
    27781 acccatgcca tgtacgaatt cctcagggaa tatctggcag gcacgcccta tgctgaccgt
    27841 cttgtcagtg ccgagccagt catctctgcc atgcgcggac gcaaaaccgc cacagagcag
    27901 gcccgtgtca aaaaagccat tgagatcaca gaagaaattt tcgccaagac ctttgatttc
    27961 atcaaagtcg gcatgaccga gatcgaagtt ggcgcataca tgcatcaact ggccaaagac
    28021 tacaacgtgg accttgcctg gcctgctgaa aattgtcctg ctgtcaactc agggccgaat
    28081 tctcccgtcg ggcacaatgg ccccaccgat atcaagatcg aacgcggaca tcttgtccat
    28141 tttgattttg gcgtaaagta cgaagggtat tgctccgata ttcaacgcat tgcctatgtg
    28201 ctgcgcgaag gagaaaccga agcccccatc gaaattcaac gcggattcat caccatccgc
    28261 accgcgatcg agaaatcgcg tgaggcgatg aaacctggag tgacaggcaa tgtcattgac
    28321 accatctcac gtgagatcgt tgccgatgca ggctaccccg attacaaata tgcccttggg
    28381 catcaacttg ggcgtgtggc tcacgatggc ggcgcattgc tgggtccgct ctgggaaaaa
    28441 tatggcgact cgcccaatca aaaactggag atcggtcaga tcttcacgat cgagcctggc
    28501 ttgcctgtaa acggctacgg ctatcttggc cttgaggaag atgtggtcat gaccaaaagc
    28561 ggcgcggagt attttcacga accgcaaaaa gagatcgttt tgttgaaggg ataagagaat
    28621 gaagcgcgag agagtatctc gcgcttttga ttcggagaga aaaatgtcac ttaatcagag
    28681 tcaggacagg ggttggaatg aagcggttct tcatcccaat tgtatgacct ccatcactgg
    28741 cagttaagtc gcgtaatgct atacttctga ttgtgaaatt tatcaccaaa aggataaaat
    28801 atgactcagg aagatcccag aattttggaa ttgagaaaga tgcgcgccaa ggctttggaa
    28861 ggcggcggcg aggaacgaat tgccaagcaa aaagccaaag gcaaaatgac agcgcgcgaa
    28921 cgtgtgattg ccctgctcga ccctggcaca ttcaatgaga tcgaaccgtt tattacacat
    28981 caaggcgatg aattggatct gctgaaggat aagttttatg gcgatggcgt catcacgggg
    29041 tatggtcaga tcaacgggcg gacggtttat ttgtattcac aggatttcac aatttatggc
    29101 ggcacactca gtgaaatgca atcgcacaaa atttgccgcg taatggatct ggcggtacgc
    29161 aatggcgtgc cgttcatttc gttgatcgac tcaggcggtg cgcgcattca ggaaggtgta
    29221 cgttccatgg gcggctatgc cgagattttc cgacgcaacg cacaatactc aggtgtggtg
    29281 ccgcagatca gtgtgatgct tgggccttgc gcaggcggcg cggcctactc ccctgccctc
    29341 accgatctgg tcatcatggt cgaaaaacaa tccttcatgt tcctgactgg gcctgaagtg
    29401 atcaaagctg tgacaggtga gatgatcgac gcagagtcac tgggcggttc gggtgtgcat
    29461 acctcggtaa gcggtgtggc ccatctgagc gtacaaagtg aaaaagcggc gcttgaaatg
    29521 gcgcgccact tcctttcgta cctgccttcg aacaatgtgg aaaatccgcc gttcacccca
    29581 acgaccgatg acctggcccg catggatgaa gagctgaatc acatgattcc gctcgaagcc
    29641 actgagccgt atgtgatgca ccaggtgatc gaaaagatca ttgaccaggg gtcattcctt
    29701 gaaatccaac ccgattgggc gcggaatgcc attgtaggcc tcgcccgcat cggcggacac
    29761 agtgtgggca ttgtcgggca ggaacctgcc atcatggcag gcgtgatcga tatcgatgcc
    29821 gccgacaaaa tgacccgctt cgtgcgcatg tgcgactgtt tcaatattcc gctcgtcacg
    29881 tttgttgact cacctggttt tctgccaggc atcgcacagg aacatggcgg catcattcgt
    29941 catggcgcaa aacttttata cgcctattca gaagccaccg taccgaagat ctgcatcatc
    30001 acccgcaaag catacggcgg ggcatacgtt gtcatgtcca gcaagtatct cggcaccgat
    30061 gtcacctatg catggccttc ggctgagatc gccgtgttgg gcgcggaagg cgcagcgaat
    30121 attttgttca agaagcagat cgaaaccgca aatgacccaa ccgcggaacg caaaaaactt
    30181 gcgaatgaat accgcgaaaa attcaacaat ccgtactatg ccgcttccac aggctatgtg
    30241 gacgacatca tcgaaccgcg tgaaagccgc ccgaaactca tcgcaaccct gtccgcactc
    30301 cgcgacaagt acgcacccgc accgccgcgc aagcatggga atatccctgt gtagaaaaca
    30361 gaaagtggaa agcaaattca ttactttcca ctttcaaggc aaatacccat gaccactgat
    30421 ctactcctct cccttcaaat caccgccctt ggtatggggc ttgtgttcgg cgcgatcctt
    30481 ttattgtggc tgatgatggc cctgttgacc cgcttcaccg cagaaaaaga atcagcggct
    30541 gaaacagact ccaccgcaca aacaggcgca tctgactcac ccgaacctgc tcccgtttca
    30601 acaagcggat tcaaagccca ggccgccgca ctggctgtgg cgctggcgct cgccgaacag
    30661 gaactatctt cagcgcatcc gctgcttgaa ccgcccacgg ccattgtctc tgcctggcaa
    30721 ttgggcatgc ggaccagtca acgctctgaa aaaggaaatt ttagaagaca ctaaccagac
    30781 ctgaaaggtc ttcggagaat gtatgaaata taaagtcaca gtcaacaatc aggtttatga
    30841 agttgagatc gaaaacatca acactcgccc cgtcattgcg cacgtggacg gtgaacggtt
    30901 tgagatcatg cctgagaatg ctggtcaggc cgaaacagga aaagactcga gcatcccaac
    30961 tgagaaaaaa gcctttaact cgtccccagt cacagccgca aaaccatctc ccaaccccgc
    31021 attgagcgga aatatcctca ccgcaccgtt gcctggaacc attgtagagg tttttgtgaa
    31081 gcctggtgaa aaagtggaag ctgggcaggt ggtggtgatc atcgaggcga tgaaaatgaa
    31141 gaacagcatc cgctcggtat acagcggcac ggtgaacgaa gtgttcgtga gtgcaggcca
    31201 aagtgtggcg cacaaacaag cgctggtcaa attcgcagac ctgggcgagg cctcgtggat
    31261 gtaacaatgt ttacaggttg caagtttcag gtttaaggat tcttatggat ctaacttccc
    31321 tacttcccga actactaaaa ggttttaccc atttcaccct cggcaatggg gtgatgatct
    31381 tcgccgcgct gattctggtg tatttggctg tattcaaaga gatcgaaccc gtcttgttgc
    31441 tgccgatcgg ttttgggtgc ttgcttgcaa atattccgct ggcaggcatg accgccgcag
    31501 aaggcatgtc aaaagttttg tacgatgcag gcattaagac agaattattc cccctgttga
    31561 ttttcatcgg cgtgggagca atgatcgact tttcaccgct gctcgctcaa ccgcgcatgg
    31621 ttctgctcgg cgccgcaggt caatttggaa tttttggcac actgatgctg gcaattgcac
    31681 tgggatttcc gctgaaccag gcagcatcca tcggtgtcat tggcgccatt gacggaccaa
    31741 cttcgatttt tgttgcgacc aaactcgctc ctgaactgct cgcaccgatc gctgtggcgg
    31801 catactcata catgagtttg atccccatca tccaaccgcc gctgatgaag ctgctcacaa
    31861 caaaaaagga acgcatgatc caaatggatt atgcgccgac accgatctcg aaaaagacgt
    31921 tgatcttctt tcctgttgtg ctgaccattg ttgtcggcct gcttgtgcca gaagccaccc
    31981 cgttgatctc gatgctgatg cttggaaatt tactgaaagt atcaggcgta gtggaccgtt
    32041 tgagcaatgc cgctcaaaat gagatcatca atattgcgac attatttttg ggtctggcaa
    32101 tcggctccac catgagcgca gccagtttcc tcaatctcga tacaggcaag atcatgatcc
    32161 tcggcctgtt cgcttttgcg ctggatacgg tagctgggct gttgtttgga aaactgatgg
    32221 cggtcgtcac aggcgggaag atcaatccgc tcatcggcgc ggctggaatt tccgcgtttc
    32281 cgatggcagg acgcctcgct gccaaaactg ccaacgacga agacccaaac aatttcattc
    32341 tcatgcatgc gatgggagca aacacagcag gccagcttgg aagcgtgatc gcgggcggta
    32401 ttctgctctc ggtcgtcagc aagatactgg gaattggata atcccactta aattaaattg
    32461 aggatgcaga aaacgctgat caaaagcaac aatcagcgtt ttctgcgtcc tacttgttct
    32521 taaaatacga acttacccgc gaaaccttaa tttcggggta agtcatgttt aatggttcaa
    32581 tcaaacttcg ggtaatcgct ggatgtgata ttggtggaag ggcgtttcag tttattcagt
    32641 aattttgtca ccagcatgcc gccgggtacg gcaaagacaa aggtcaacaa acctgaaaca
    32701 accgcaccga tcaatgccca gatgccgatc caaagaaagg catagaagat cgggtcgctt
    32761 ttcacaacag ttccgctcgc atccacacaa tgcagttcgt atgcagtgcc attttctgga
    32821 ttgccaaact catcgaaaga agtgcttgag taggaataac tcttcggtgt tgtgccagca
    32881 gggcacagaa aaccacccgt tgtcttaatg gcatattcac taaacgatgt aaaactcccc
    32941 accataaaga acactggcat cacacaactg ccgatgacgg aaatcagtaa aaaccagatc
    33001 acacacccgg aaatggcggt cgttttggtg ttgttcatat caacctactt tcattttgcc
    33061 atcatacatt gattcgtgct ttacctgttt ctgcggagca aacacccggg caagcacaat
    33121 cacaaacggt gaaaggatcg ttgcaagcaa caacacagtg aaaaccgtat cgatattatt
    33181 ttccacggga gcacccgaga gattcacgca atcgaaagaa tatcctgtgc cttcccggat
    33241 gaaaccatct tcataccagg ttgtgtcgct cgcagtcacc ttgagtatgg aaccagatgg
    33301 gcataccaac atccctgcaa ctttccgcag cgggttgttg ggaacaagcg caatggatgt
    33361 aaccatcaag gcaatcacaa caggaaacac aaagattaac caccaaaact tcatagcctt
    33421 cattaattca cctcaataaa ttatttttgc aagccagatt atagactgat gaaacccaaa
    33481 caaaatcacc tgtttgtacc aagtgtgcag atagttgtat tgaactcact tgtgcgctat
    33541 aatcaaagtt gtctgacaac tacttcagga gaaccccatg accacgattg acctgaccgt
    33601 tcataaagac cgccgcgccc gcgccattca acgcgcaaaa gagcgcaaca tcatcatccc
    33661 cacctatgcc caaatgaaag acccttccaa aattcctgcg aaaatcaagg aggaattgac
    33721 caagatcggg ctatgggata ttcacccccg caaccttttc cgcatcaact ggcataacca
    33781 acccaccgct tcaggcggaa cctttggcgg ggtcaactat cttgagcttc cctcttcgct
    33841 gacaggagtc aaagcccgta tcattgccgt tgtcggcaaa tggttcccca ccggcgcaca
    33901 caaagtcggg gcggcttttt cctgccttgt gccgagactg gtcacaggtc agtttgaccc
    33961 gacgacccaa aaagcggtgt ggccttccac aggcaactac tgtcgcggcg gtgcatatga
    34021 ttccgccctg cttggctgcg aatcgatcgc cattttgccc gaaggcatgt caaaagagcg
    34081 ttttgaatgg ctggccaaag ttgcaggcga aaccatcaaa acccctggct ctgaatccaa
    34141 cgtaaaagaa atttttgata aatgcaagga actggctacc tcaggtcagg acttgatgat
    34201 cttcaatcaa tttgaagaat ttggcaatta tctctggcac tatgaagtga caggccacgc
    34261 catggaagaa gtcttcaaag cggttgcagg caagcatgga cgtttccgcg gcatcgcctc
    34321 tgcaacaggt tcggctggca ccatcgccag cggcgactac atgaaacaac tcttccctga
    34381 aagcaagatc gtggcgagcg aagccctgca atgccccacc ctgttggaaa acggcttcgg
    34441 cgcgcatcgc atcgaaggca tcggcgataa gcacgtgccc tgggttcaca acaccaagaa
    34501 cacagacatt gtcaccgcca ttgacgataa cgccgtagtc aacatctccc gtctgttcaa
    34561 tgaagaaaac ggacgcgcct acctcgctga aaagggtgtg cccgaatccg tcatctccaa
    34621 ccttgacctg ctcggtttct ctggcatttc aaacgtcctt tcctgcatca agaccgccaa
    34681 gtattacgaa atggacgaga acgacgtcat gatcaccatg ttgacagact cgatggaact
    34741 ctatcgctca cgcctgcatg aaatgcacca ggaattcggc gaatacagcg aaaaagacgc
    34801 cgccgccgat ttcgcccgct acctgcatgg acaatccact gacaacatgc ttgagcttac
    34861 ctaccccgaa cgccgcaggg ttcacaactt gaaatactac acatgggttg aacagcaggg
    34921 caaaacctat gaagagattc aaaaccaatg gtatcagcct gattactgga ccagcgtcca
    34981 aaagcaggcc aatgaaattg acgaattgat cgttgagttc aacaaggaag ttggattggt
    35041 gtaagacaaa aaatcctccg agttttcagg ctcggaggat ttttttattt gacctcttgt
    35101 ataagtaaag tgttatagac cacaaagtca ccaaggcact aagtcactaa gatttttttc
    35161 tttgagtctt cgagactttg tgccttcgtg gtaaaaaaat ggacttatgc aagagttcta
    35221 tttatgcaat cggatgccaa agcatcgaaa ggatgagagc caataatata agcacggcac
    35281 cgatcgcagc gaaaaacaca ctgacctccg tcgtttcagt gacggtcatc aattgcacgg
    35341 gcaggttatt gaaaacattc tgaagctctc cggtgctgga agccgcaaaa tattccccgc
    35401 ctgtcatatc ggctacttgt ttcagggtct cttcgtcaat tcccctgcgg aaaccaccgc
    35461 cacccccacc tccgcctcca ccaccgcccc cgaagaagaa ctgattgggg tcattcgggt
    35521 tgcctccacc ttgaaagccg ccgcaattca tgggggcatc ttcctgagcg gttccaaacc
    35581 cgatcgtata aactcggacc ccgcgggtct tggcaaactc cgccgcgaag acgggctctg
    35641 gcccagtggt gttgacgccg tccgtcaaca ggacaacgat gtacggcgca tattgccctg
    35701 caggcatggg ctctggctct gtgcctgcga acggacttgg catgctatcg tcaatttcgg
    35761 aaagcgcttc aagagatttg agcaagcccg agccgatggc tgtcctgcgg gcggtggtga
    35821 tgttttgaat ggcggtttca agcagactgc gttcggtggt tggctcctga atgacgaccg
    35881 cataccccgc aaatgccacc agcccaattt gcacgttcga ttcctgcgcg gcgataaaat
    35941 cccatgccgc tgttttggct gcttcgaggc ggttcggaga gatatctgtc gagcacatgc
    36001 ttctggaaac atcaatggca aggatgatgg tggcatttga agcgggcaca gtgatcgtgg
    36061 aaacgggccg ccccatcgcc accaccaggc tcgacattgc cagcaaaaac aacacaaacg
    36121 gcagatgacg ccgcacctgt gattgttttc caagcgcctc acgcaccaaa gacaggcttg
    36181 agtaacgcac cgcatacggt tttcgtcggc gaagaacaaa gatgtaaacc agcaccaaca
    36241 aggggatcag tgccagcaaa taaaggaatc caggccagag caattccatg tcagcctcca
    36301 atctcaccac ggagcacaca gagaccacgg agaatttcaa gaggcttttc tctgtggact
    36361 ccgtgatctc tgtggtgaaa aaatagaaaa ctaaaccaaa taccagagag tctaaaaaag
    36421 ctatggcaca tggctaaacc acaacaggga gagcatccct gcaaccaata aaatgagaat
    36481 gctcgcaccc gcaaaaatgg cggtgacttc cgtcttttct tccacgatca ccaattgcgg
    36541 atcaatattt tcgtagatgg tttgcagatc ttcctcggtt tgggcattgt aataatttcc
    36601 gtctgtgatc tcggcgatct gccgcaacgt ggcttcatcc aactgcgtga agaccgaaaa
    36661 gccattcacc tccagttgaa cgcctgcgct gctgccaacc ccaatggtgt ggatgcgcac
    36721 gccgcggtcc gcggcgaatt gagcggcttc gagcgggtct gggtccatgt tgttttcgcc
    36781 atcggtgagc aacacgatca cagcggaatc atctgcactt gaaacaggag caggcgtggg
    36841 cgcaggagaa gaagcgtctg gttgggggac atccatgccg aggatgggag gttgtcctgt
    36901 tacgttggca atcgtattta acgagatgat gatcccattg gcaagcgaag taccgcgctc
    36961 ggggcgcaaa cggtcaatgg aagcgaggat ggcttccttg tcgttggtgg gcaattgcac
    37021 cgaaaaaccg ttgtcgctga aagagacaat gccgatcttc accgtctctg gctggcgttg
    37081 tacaaaatca cgccccaccg ctttggcggc ctccatgcgg gtcggctcga aatcggcggc
    37141 ggtcatactg cctgaaacat caaaagcaag tatgaccatg ccttccacct tcggcaggct
    37201 tgccaccatt tgagggcgcg ccaatgaaaa aaacaaaata gccaggccaa tcaaaaacaa
    37261 aattgcagga aggtggcggc gcgaactggc agcgcgtccg ctggtttgct gcaccaggcc
    37321 caaactgcca taacgcacag ccagttgttt gcgctggttt tgcatgcgaa gatacaacaa
    37381 agccagcagc ggaatcgcca gcaaagaaaa aagcaacgtg ggccagagaa aagacatggt
    37441 taccttttgt gttgacgccg catcatcacg aaacgcgcaa tggcgtggat cagatcttcc
    37501 tctgtggaaa gcggaaggac atcgacccct gcgcgcttga aagaatcttt caacgcagtt
    37561 tcacgttgtt gcgcggcggc gcggaaacgt tcgcggaatt ttttatcgtg cgtatccacg
    37621 tataactgct cgcccgtttc tgcatcttcc atcacgatcg ggccgatgtc gggcaggtcc
    37681 atttcgcgcg ggtcccacaa gcggatggca atgacttcgt gatgctggct caacagattc
    37741 aacgtgcgct cccagccagg cgtgctgatg aaatcagaca ccacaaaaac gagcgagcgt
    37801 tttttgatgg catgcaaacc gcctttgagc aatgcgctca gatcggtcat ggaagaatgc
    37861 ggaagacgcg gctgattcaa caggtcgttg atgatgcgca gcacctgcag ccgcccgctt
    37921 cttgctggga ccgtgcgctg gatcgcgctc ccaaacatca tcgccccgac gcgattcccg
    37981 tgacgggtca atagccttgc aagcgtggcg acaaaatcaa tcagcacatt gcgcttctga
    38041 ttttgcagcg tgccaaaatc caccgatgga ctcaggtcga gcaaaaacca tgcaatgact
    38101 tcgcggtctt ccatgtattg acggacgtac ggcgtgttca tgcgcgcgct gacgttccaa
    38161 tcaatgtagc ggatgtcatc ttcaggctgg tattcgcgca ggtcggcaaa atccacgccg
    38221 aagccgcgga agagactgcg gtaatcgccc tgcagaagtc catccaacct gcggatgacc
    38281 tgccaatcga gccgatgcag aatgcgctca ggcgttggcg tggacgttga agtgttcatg
    38341 cagtggcacc acagggagcg gaatgcgttc gaggatcttc ttcaaaatat cgtcgctgct
    38401 gatgttgtcg gaaagcgctt cgtacgaaag caccagcctg tggcggatga catccagcgc
    38461 catgtcgagc acatcctgcg ggaggacgta gctgcgtccg cgcacgaagg cgagcgcctt
    38521 ggcggtgagg atcaaattga tggatgcacg cggactggct ccgaacatga tgtagcgttg
    38581 aacctcgccc aggcccacct cttttggatt gcgcgtggcg ttgaccattt tgaccgcgta
    38641 ttcaatgagg gctgggtcca catacacctg atcggtcctc ttttgcaatt ccaccaactg
    38701 gtcggtggtc agcacttttt gcacttccgt ccacgcgcct gtcattcgtt cgacgatgac
    38761 aaattcttcc gttgggctgg ggtagcccac caacaccttg agcatgaagc ggtccacttg
    38821 cgcttcgggg agtgcgtacg tgccttcggt ttcgatcggg ttttgagtgg ccagcaccaa
    38881 aaacggattc ggcactttgt gggtctcgcg gccaatggtg acctgttttt cctgcatcac
    38941 ttcgagcagg gcgctttgca ctttggcggg cgcgcggttg atctcatcgg ccaacaataa
    39001 attcgtgaat acaggcccaa gcgaggtgtt gaactcgccg ctcttctggt tgtaaatgcg
    39061 cgtgccgatc agatccgcgg ggacgaggtc aggtgtgaat tgaatgcgtt tgaattcgcc
    39121 gccgatggaa tcggcgaggg tcttgatcgc catcgtcttt gccaggcctg gcacgccttc
    39181 cacgaggata tgtccgcggg cgagcagggc cacgatcatg cgctcgagca aatggtcctg
    39241 ccccacgatg actcgcttca cttcgtacaa cacgcgttcc attgggagtt tgttctcacg
    39301 gtttgaggtt tgttccatgc ctgtgctcct tttgtctctc agtaaatatg taggggcgac
    39361 ccttcaatag ggttgaagtc aactgtcttc cagaggcggg tcgcccctac agtccagaaa
    39421 atgaattaat acggcggcga gcccatgccg cccaccgcca cggtgatcgg caccgcaaac
    39481 ccgatgccga cgaaaaaact ttcatcggtg gggttgatga tgccagtgac aatgccaatg
    39541 acatgaccat ttcgattcaa cagaggcccg cccgagttcc cagggttgac cgctgcgtca
    39601 atttggatca tgcctgggat ctccaaacca gtcccttcca tctgaaagac tcggtcaaag
    39661 ccagagatca cgcccgagct catcgagctg tacaaaccaa aggggttgcc cactacaaat
    39721 gcctcatccc ccacccgcat cgaattgggg ttgcccaaca cggcaggcac caccgaaagc
    39781 ggcgtatcgt atgcctgcag caccgcaata tcacgctcag gcatttcaga gacgatcgcc
    39841 gcctctgact gcgtgccatc ggcgaaggtg accgtgatca cgctggcccc atccaccaca
    39901 tgcagactgg tgagaatgtt gccgaaggaa tcgaccacca cgccgcttcc caacccgaaa
    39961 tctgattcag cgttctgatg cttctcttcc acctgaatca ggatcagcga cggccgaatg
    40021 gcctgataca cctgcgcgga ataggacggg gcaggcgtcg ccgaagccat gaccatcgcc
    40081 accgaagcgt tgacatcttc cacggtcagt tgatttggtt ttacaacaag cagattgtag
    40141 aaaaacagga caagcaaagc accaaagacc cccgaagcaa aaggcagccc gcgccgcaaa
    40201 cgcgcaatga cctccctcat gcgacctttc catttatcca tttttgaagc agacgattcg
    40261 ttcatgcaca gttcctgatc aagtaagctg gcgtacacga catttgccaa tataaggact
    40321 ttattttaga taaagatgag aaaagttgtc acattcttac agatttacaa aacccacata
    40381 attacacccc caggtgccga ttttcaatac ggggtgggta taattgccgc atcacattca
    40441 aactgtaagc gattcgaaac atgagggctt tccatgaacg gtcattgctt catcagttat
    40501 tcaacagctg acgctttgga ttttgcacgt aaacttgccg atgaactcga aggggggcat
    40561 ccatatatcc gtgtctggtt tgataagcgt gatcttcgtg tggatgaaga ttgggatgac
    40621 caaatttcaa gtgcaattga aacctgtaca tgtttaatat ttgttttatc aaacgatagt
    40681 acatctcaga cctcagtttg caaagaggag tggtcgtggg ctttgaaata caagaagccg
    40741 attatcctca tacgtattca aacaaatgtt caaccaccct ttcgtttggg ggaacgtaaa
    40801 atcattgatt tttctgcaga ttttattata ggattagcaa aactccgcaa gcaattagct
    40861 tatcttgatt caccagaagg aattctcagt gaactaaaac acagtcttgt tgatgcagaa
    40921 agagatctgc accgcacaaa aggcaacgaa gaaaatctta tcaaatctga aatcagtgaa
    40981 ttgaagaaac aaattgaggt tcagcaaaaa attttggata atccacaagc cttacaaaag
    41041 caaattcaaa agaacgtcaa tgccgaactt gaaggagaac atcagcacct gaaaagtgag
    41101 agatccgtta atcgtatttt tctttcatat cgcagagcag acagtgcgga tgtggtaggc
    41161 cgaatctatg acagactaat ccaagtatac aaaaaagaat tcgtattcaa ggatgtagat
    41221 tcaattccat taggattgga ttttcgagaa tatttacata gaatggtttc aatttcaacg
    41281 gtattcatcg ctgttattgg aaaaaattgg gaaagtgtgc aagatgaaca aggaaatcgc
    41341 cgactgaata atccggccga ttatgtaaga attgaaattg cctcagcgct ttctcgcaac
    41401 atcccagtta ttccagttct tgtacaaaac tcttctatgc cattagttga cgatttacca
    41461 gacgacttaa agaatttatg ttacaggaat ggtatttccg tacgacctga cccagatttt
    41521 cacaacgata tggatcgtct tattagtgga ttatcgaagc tagaagacat caataacaaa
    41581 aactttttta agtagtgatt gcagtagaaa gaaaatgtaa tgaaaaatca ttgcttcatc
    41641 agatattcaa cataggtgtc cataaatgtt aatttatttt atttttcctt taattgtcgt
    41701 gttgattgga ttgcttatcg aatattgggt tatacaacca ttacgcaaaa acggaattcc
    41761 cattagcttc caaacgacag tttcaagcat aattcagaga atgacgtcat ttataaagcc
    41821 tgacaaaata aacgaagaaa gttcgccttc cgaaaaaaag acttccttga ataggatact
    41881 aaaatctaag ttgggcattg gtatcacttt aattatcttt ttgttattat taactgcaag
    41941 ctatttttta attaaagcaa atcaaaagcc agtctacacc taccctactc ccataagata
    42001 ttcaattgca attggcgata acttgaataa agttatagat atcgatcaaa ctcatgaata
    42061 tgtttttaca tcgagctcca ataaaattgt ggctatcaca attagacgaa ttagaaccac
    42121 tgatgaagcg aaaaggaacc tgagagtcaa tctatacgac gaaaactatg tctatctatt
    42181 tgaagaatca atacttgcag ataccacgga cccagctgaa aaaacaattg agtttgcacc
    42241 agaaataggg gtcaattatt atcttgaaat ttatttaggc tcctctccca gcaatgatta
    42301 ttttggcgcg aactacatta tcagcctaga atataaatag cagtaaaggc actacataat
    42361 atgcacatgc tctgttgtaa acataggttg aatatgttaa gtgagtattt atgcatcaac
    42421 gagaaagccg cattgcaaca tgatatacca caatgccagt gctctgcacg gaatcatcgc
    42481 ctcccgtcaa ggggagcaag gggacgaggt cgcggcgcgg ggagcctttg tccgcgcgat
    42541 cgggcaggcg gatgagatcc tgtccaaaac ggcggagtat tattccgcgc tggatgcgaa
    42601 ggggttggcg atttgcgggc tcgtgctctg tcggggcgca gcgccgctgc gcccgtacgc
    42661 ccaagatata gacaatgcca tcgaaacatt caagaaggcg cgaagaatcg cgccgcacgc
    42721 gggggtggtc aagtccgtgc tgcggttgtt cgatgaattg gcaaaatgcg acgagaagga
    42781 tgttttgaaa gggacacgag aggttttaga aggaaaggga acataaccat gtttggtcta
    42841 aacgcacatg aaattaattc aatagtcttt actagcctta caagtgcctt aattactgga
    42901 gtcattgttt atttaattca aaaatctatc gaaaattcgt ttaataaaaa aatggaggaa
    42961 tttcgggcaa gtatccaata tacttttttt gaacaacaga caaaattcac gagaacattc
    43021 caaaaaaaga tagaaacatt cgaggtttta caccaaaagc taaatcttct tgggcaaagc
    43081 atgaacagtt tagccaattt aataaaaaac gaaatcacgg catcgtccca aaaaaaatca
    43141 gaagagtacg acaaagcaac tgaaacggtt tcaacgtcat atttagattt tttacattat
    43201 tacaccgaaa accgccttta tctacccgac caatcaattg agacaattga aaaaattagc
    43261 acaaaggttg gaatgctaag tatgctaata ggtttatcag gcatgatctc tacagccccg
    43321 cttgaatcag taacatgggc aacatcctgg gcaaatcgcg agataaaaaa gattgacttg
    43381 gatattaaaa gtataaatcc aaagaagcca aatgtacctt tgtttattga ccaaataata
    43441 aaagaaatga aataccaaat agaagcttta gaaaagcact acaaattcac gacaatggat
    43501 gatagcacaa gcatttcaac gagaaaattt taacgcgcaa aatcgcttcg tatgtggaaa
    43561 tcgtcaagtc catactgcgg ttgttcgatg aattggcaaa atgcgaccag gatggcgtgt
    43621 tgaaggatgt ccgaaacgcc gccgaaggaa aagaataaat tcggtagggg cagtcgtgcc
    43681 cctacatttt taacggagac cccatgaacc caatcattct tcacgcagac catctggttt
    43741 tgatggattc aaacaacacc gtcctgcaaa acggcgctgt gcttatcggc gcggatggca
    43801 ggattcagtc cgtgggacag gcggatgcgg tcatggccgc caaccccagt gtcgaggtca
    43861 agcggctgga gaatcgtctg ctcatgccgg ggctggtcaa cacgcacatg cattcgggtc
    43921 tcctgcgcgg cacggccgaa ggtctgcccg tgtgggaatg gcttcagcaa tttattgacc
    43981 caatgcatcg cg
//
LOCUS       DAOQLC010000017        43684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_247_length_43684_cov_23.912375, whole genome shotgun sequence.
ACCESSION   DAOQLC010000017 DAOQLC010000000
VERSION     DAOQLC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 43684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_247_length_43684_cov_23.912375"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="PL000_04780"
     CDS             complement(<1..2313)
                     /locus_tag="PL000_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88242.1"
                     /translation="MNQTNTQLKRVSRPRWSIRFTSLRTRLLVTSILLVLLPVSITGA
                     VATYISSQGLTADVFDQLESVVTLKESQVHDWLASLQTNLDMVFVNVDSLKRVSELVL
                     NQEPDEQQKDLVRTELTNNSQRSGYFTEIFVMDKDGQVIVSTESNQEGKILTTQSFFH
                     EGIKGRYITPPSYELSLSNYTIVISQPLVSNTGKTIGVVAGRVNLNTLDEIMQQRTGL
                     GETGETYMVSSNFAVLTKLRFGETVLGQTYIHTDGVTNAIKSKANGSGSYPDYRNTAV
                     AGVYHWVPELQVAIIAEQDESEALSAASRVFQVTLGLIVITVLAAGFAAFLTTQSITA
                     PVTKLATAAENIAKGNLDQHVDIIQQDEIGVLANSFNSMTSRLRELITTLEQRVADRT
                     KALATSSEVSRRLSTILDQRQLVTEVVEQVKNSFNYYHAHIYMYDEEHGELVMAGGTG
                     EAGATMLANGHKLSKGQGLVGRAAESNEPILVNDTSHDPNWLPNPLLPETKSEVAMPI
                     SIGNKVLGVLDIQHNIVDGLKREDVDSLQSIANQVAIALQNIGSVETMTKRAKELASV
                     AEISTVSSREADINRMLENVVYLTQRRFGLYHAHVFLYDRNANHLQIIACGWKEGDEH
                     EGTHGTTIIPLLQEQSLVARAARTKQAVIINDVHNEPGWLPNPLLPDTASELAVPLLV
                     GDQLIGVLDVQSDRLNAFTEEDANIQTTLASQIAVTVQNARTLIQSKRQAERETLVNI
                     ITQKIQNATTVEDALQVAARELGHAMGMKST"
     gene            complement(2427..3437)
                     /locus_tag="PL000_04785"
     CDS             complement(2427..3437)
                     /locus_tag="PL000_04785"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC88243.1"
                     /translation="MKTLNRFSKLAVFTTWITILSLALSGCGNMNFSAPDKVTVQLSW
                     FPSVEYAGFYAAIENGYFKDENIEVTLIPGGPEVNPLAEVESGKSQFGITTGDSLIIA
                     RTNNQNYVAVATIFRQNPLVVMTLDSSSINKPEDLAGKKVGVIAPDLSTTYDIQFLAL
                     LRTLNIDQNSMTFEAIDDYNGASELITGGMDAMSGSFATNEPIQATMAGNDVSLIYYK
                     DYGVDVYANVLFTSEEFLQNNSDLITRMVRATMKGYQFSIENPDKAAEFALKYDPTLD
                     LAFQVNVMTAQIPIIDTGDAPIGAMDGDVWETTQQILLDFNLITEPIELNTVYTNQFV
                     GQ"
     gene            complement(3477..5825)
                     /locus_tag="PL000_04790"
     CDS             complement(3477..5825)
                     /locus_tag="PL000_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88244.1"
                     /translation="MPWMIPPETTPTSTNPVRILVVDDHPNTATTLARALSQLGTGVS
                     VISASSGHDALEKVKTNGVDILFTDMIMPEMTGLELIEKMQNHPAGRPSYTYLVTAYD
                     VPGLKVTAHRLKVNEVIVKPVRPERICQIATNAIEEMKHSTHPIKQSSVLRKKFKILV
                     ADDRPDNVTLLTRYLEYEGYEHIAASDGLDTLDKVRAELPDLVLLDVNMPHKDGFAVL
                     EEIRSDPSVQHIPVIILTAARLDPSDVQSGLNLGADDYVTKPFDRHELMARIRTKLRV
                     KETEDIIRRRNRELNLLPEIGKELSARTDIKDLANILIKRTVETLGAIQGNMIIFGTN
                     GGPAQNYQVPAASPDSANSMIAIPKNIIKHVEESHQGLIIADARNNSLWQLDNQDSNI
                     HSAVISPLFGRMELLGLLVLTHEQVNYFNTDHLLLLQAITSQAAIAIENARLYSIAMQ
                     EQNRLSAVLKHAAEAILVFDAQGKLSLLNPEGEKLFTDFSTNLNQPLPHDHGYDVFIH
                     MLDDARTTHGAKSGDITWPDQRTFNALITPIEDGGQVAILHDVTRFKDLEQVKNEFIA
                     TASHDLKNPITTIAGFSTLLEHAGPLNQQQKDFVTRIQTATHTMSELVQNMMSLAQMD
                     LDATQKHEVVDMDKVMTEVVNEFIPQANIKNQRFSYTPLEQPVTISGDPLQLRQLLRN
                     LLGNAIKYTPTDGEITFKTQASDSQLLIEVKDTGCGIPAADLPFIFDRFYRVRSGHNI
                     EVEGNGLGLAIVKSIVEHHHGQVNVESEVDKGTSFTLSLPLS"
     gene            complement(5987..6778)
                     /locus_tag="PL000_04795"
     CDS             complement(5987..6778)
                     /locus_tag="PL000_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88245.1"
                     /translation="MSNGHILIIDDEATLRQSLARILQQAGFEATTAENADQGLAFIE
                     TTLFDLVFMDLRMPGIAGLDALKLIHANYPTLPVVLFTAQPDLNSAVEALRSGATDYL
                     LKPLKPQIVIENAQKIIASRQKEKRKLEIMQQIENLQAELKGLNNGDTGPLPATRVDA
                     NRYIKRGTLVLDLHKRCLNINEHAVNLPPTSFEYLLVLARHSPDVVSYQTLVTEAQGY
                     QADLHEAQELSKWHIHQLRQAIEPDIHSPTYLINIRGTGYRLVAD"
     gene            complement(6775..9366)
                     /locus_tag="PL000_04800"
     CDS             complement(6775..9366)
                     /locus_tag="PL000_04800"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88246.1"
                     /translation="MESSQAKWLFTGLSAVIYLIGSILLFNYFGVAITTLSGLPVVVS
                     AYFFGIPGGIIASLLTILVNTVLLVNVQKIPWAEINGYDYGLGAILITLLGISVGWIK
                     YKYEEYRSILSQLRSRERFLELLNDMAHAITTAQNFESMANTLTNDITTLLDADSCYI
                     TRWDPVKEQVFPVATNNLKNTAFLTMEYPKGEANLTITALQENRIIVVEDPHHSTYVS
                     ATLIHLFPEKSFISIPLNYGDHKLGAVIVGFAEPHQFTEEELNRAEQAGNQIALAIWN
                     LQQDNELQRGLREMQSLADISLALSETERIGLSNVLHLIVKSAKDLIANSEQAVIHLL
                     NEKDQILVSEAVVGYTELEGGKRNIRLGEGVAGQAILSGETINIADVRTDPRFLRLGV
                     EPAYRSLLVTPVRSGEQKLGTISIQSSRPYAFTPSDLKLLSQLGTQAAIAIENANLLE
                     NTKQALKEANALYRVNKGLVASLDPHDLLQDTVELLQKNFGYHYVQIYVANPDTGNFI
                     MRAGSGKVGALLKAREHQLAPGEGIVGYTAETGTAFFTNNVDDVVSFVRHPLLPDTKS
                     ELAVPVKVGKQILGLLDIHQVPPKYLSQRDLQLVSAVADQLAVALQKADLYETLQISL
                     QQEKTIRNQLMQNERLTVMGRLLATVSHELNNPLQAIQNALFLLKEETNVSPQGKQDL
                     DIVLAESERMANMIARLRDTYRPTHAEDFQPTQLNDIVNDVYALVSTHLRHNQISFEF
                     TPDPAIPFIPALADQIRQVVLNLLMNASEAMQNGGRIKVCTEYQKDTGEVVMIMSDNG
                     PGIPPKILPTIFEAFVTDKQSGTGLGLTITYDIIMKHRGRITAENNHDQGATFRVWLP
                     VTSGEIL"
     gene            complement(9558..11084)
                     /gene="rny"
                     /locus_tag="PL000_04805"
     CDS             complement(9558..11084)
                     /gene="rny"
                     /locus_tag="PL000_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNC88247.1"
                     /translation="MSPLVLIIDLIVLIVGVSAGYFFHRYQAEQAAKARQEKADDILK
                     AANSQARLIESGARENAAKITKAAEAEVKERRIELNKETERLDKRRSELDSRFDKMEQ
                     REQTLNKRQSQVDKRANEIDKLYDDQMKKLEFIAQMTQDEARKELFALVEKEARGDMA
                     RIIRQIEAEAREEGEKRARKLIADAIQRVASEHVAEVTRAVVTLPSEEMKGRIVGRNG
                     RNIKAFEQAAGVDVIVDDTPDSVTISCFDSVRREIGRRALSKLILDGRIHPAHIEKIV
                     EDETKAVEKIINEAGEQAAYEANVTGLHTEVLRMMGRLKFRTSYGQNQLAHAVEVSKL
                     AGILAAELGANIELAKQGGFLHDIGKAMDHNQDGTHAGLGAEYCKRYGVHPTVVNAIA
                     AHHHEVDQETVEAVIAEAADAISGARPGARREDLEAYIKRIRTLEEMSMSFEGVQQAF
                     AIQAGREVRILVKPDTIDDLSSARLARDIAKKIEETMQYPGQIRVTVIRETRATEYAK
                     "
     gene            complement(11081..11746)
                     /locus_tag="PL000_04810"
     CDS             complement(11081..11746)
                     /locus_tag="PL000_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HNC88248.1"
                     /translation="MKKITAISVQKKTPNRVNIHLDGEFAFGVARITAAWLKVGDELS
                     DEKIASLLSEDSRERAYQQAMLFLSYRARSEKEIRQNLHKHEYSEDVVETTIERLRDA
                     GLVNDDEFAQAWVENRSTFRPRSRRALAMELRQKGLNDETVQTAVSAVDEETLAYEAA
                     QKRLGRLKGLEWNDFRKKLSEFLARRGFPYSIIAPVVTRAWSETHPTGEQKIFDDENE
                     EIT"
     gene            12247..14214
                     /locus_tag="PL000_04815"
     CDS             12247..14214
                     /locus_tag="PL000_04815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC88249.1"
                     /translation="MTEQQQEATGGDIGRIRRFIAFLGILFILVSQFLIFSQPVDHTL
                     VFPPYTWLAIVGVLVLALGLFIRPTAFFQRISDWFVFQERTFWVLAAFLMSALVTMAT
                     ANLMLFARINYIPVLTIWLLSAWAYIYAFSNFSISGSAIFDWFKKNRNELLAVLAVTL
                     LAAGLRFYRLGGIPRILDGDEGLIGLAAQTTTSGLLANPFSLWENFGAMYLHLINTAI
                     SFFGVNAFALRLWPAIGGTLAIPSLYLFVRWIGGRRIALITALIIAFSHSHIHFSRIV
                     SVAYIHGTWLAPLELYLMLSGLEKRQSWRAALGGVLIAIHFSIYLTAQVIIALAFVFM
                     VLAFLFYRPWFKERFAQAMAFWGGFLIIILPSAFYIYRNPNEFFNRLAQNGTFQSGWL
                     ELTMQSTGQSAAEILFGRFVHAFLSLTYYPAIDFYGSSSPMMSMISSTMFLVGLLVAL
                     WRIRNPAYLLLNGYFWGATFSVGLFAIPPNSDSYRMLMALPSALTMAAMGLDQILELL
                     GLGWNKARTSYTLSASAILVSLLIFNLWTYYGDFAGKCRFGGDLVGRFASYLGSYAHS
                     VDNELPIYLLSNGIYFYGSHASTDFLSESRVITNFPDPIDTLQPVSGETIIANPERIP
                     ELEAWARLNPGGELHYRYDCSNTILLAYRVP"
     gene            14222..14956
                     /locus_tag="PL000_04820"
     CDS             14222..14956
                     /locus_tag="PL000_04820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC88250.1"
                     /translation="MDPREYELMYQVEDRHWWYRGMETITRALLERWAPARPQRILDA
                     GCGTGAAMTTYLTDYGKVTGIDLYAEALKFCRERNAPRLARASVLDLPFAPASFDMVT
                     SFDVLYERAVSDELTALKEFFRILTPNGCVLLRLPAYDWLRGQHDRNVHTNRRYTKKL
                     VRHLLEQSGFTVEHLSHANTFLFPLAVMKRMGERVFPPKDNQSDLAMNAGVFNDALKI
                     ILSAEAPLVSSIGLPYGLSVIAIGRK"
     gene            14965..15708
                     /locus_tag="PL000_04825"
     CDS             14965..15708
                     /locus_tag="PL000_04825"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88251.1"
                     /translation="MKHERESQQLPSISAVFPAYNDGGTIASMVAAAWIALNQVTSDY
                     EIIVVNDGSSDYTATMLDEISVRYPELRVITHPYNQGYGAALRTGFSAASKDWVFYTD
                     GDSQYNPLELVNLVGALYEGVDVVNGYKLTRNDSWMRIVIGRAYHYLVKFLFGIHIRD
                     VDCDFRLIPRRILEEIELKSVSGAICLELVKKIEDAGYVFAEVPVNHYSRKYGVSQFF
                     VPWRIARTLYQLADLYWKLVIRKEHLIKR"
     gene            15705..16703
                     /locus_tag="PL000_04830"
     CDS             15705..16703
                     /locus_tag="PL000_04830"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC88252.1"
                     /translation="MTIMDLAAAYFRQKVLITGGAGFIGSNLAHKLATLGADVTIVDS
                     LDPDSGANLFNLNGIEKTVRLIQADINDERQMAQAVRGQKFMFNLAGQTSHLGSMQDP
                     FKDLDVNSVGQLRLLEVCRQNNPEISIVFAGTRQVYGRPKYLPVDENHLLTPLDNNGV
                     SKRAGEMYHIVYNRVHGMATCVLRMTNVYGPRMRIKDDLLTFIGWWFHQLLMGENIQI
                     FGDGRQVRDINYVDDVIDAMLLCAAHPSANGKIYNLGGAPINLLDLARLMIQVNGKGG
                     YTLVPFPENRKRIDIGDYYGDYSLIQKDVGWRPQVELETGISNLLAFYHMYKEHYL"
     gene            16709..17815
                     /locus_tag="PL000_04835"
     CDS             16709..17815
                     /locus_tag="PL000_04835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC88253.1"
                     /translation="MEEIPVIDLKKQYQSIRPELDAVFARVMEKGTFILGSEVSAFER
                     EFAEYCGAAHAIGVASGTDALQLALMACEIGENDEVAVPAHTAVATVSAVEAGGAHPL
                     LVDIDSTNYTLDPNLLHRILMPHTRAVIPVHLYGCPVDMEPVLQFARENHLFVIEDCS
                     QAHGASYRGRKVGTWGDIAAFSFYPTKNLGAFGDGGMVVTNNDALAEKVRLLRQYGWG
                     EHYISSIRGINSRLDELQAGILRVKLRHLDGWNARRRQLAELYHHLLSGTDLVLPAQN
                     AGHVYHQFVIRHAQRDSLREYLKENGISTLIHYPVPVHFQPAYADLGYTAGSLPFTER
                     ASREVLSLPLYPELTEEKVVRICQTILDFFTHSM"
     gene            17997..20264
                     /locus_tag="PL000_04840"
     CDS             17997..20264
                     /locus_tag="PL000_04840"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNC88254.1"
                     /translation="MAKITREDALEYHRLKGKPGKVAIVPTKPMDTQRDLSLAYSPGV
                     AEPVLEIEKNPDDAYEYTSKGNLVAVISNGTAILGLGDRGALAGKPVMEGKGVLFKKF
                     ADIDVFDIELNSHDPDEIIKVVAAISPTFGGINLEDIKAPECFYIEEELKKMLNIPVF
                     HDDQHGTAIISSAGLANALEIVGKRHDEIRLVISGAGASAISCAELAISWGVKRENIM
                     LCDSKGVVYKGRKEGMNKYKERFLVETDARTLTDALRGADVFYGLSVANVMTPEMVKL
                     MAKDPIIFAMANPDPEIKPELAREARKDVIIATGRSDYANQVNNVLGFPFIFRGALDV
                     RAKQINEEMKFAASKALAALAKEDVPDSVIRAYGGESIKFGREYIIPKPLDPRVLLWE
                     APAVAEMAMKTGMARKTIDIEEYKEQLAFRQGKGERIRYFFQNKARTSGGKKRVAFAE
                     GEEQKVIRAAYQIKEEGIATPVLIGNAGVIDEQLKVLGLDYKPVIVDPNKFEKLEAYA
                     KSYYEMRQRKGVMLSDAHNKVRDPNILGPLMVKMGDADAFVSGLTFDYPEVIRPALQI
                     HHTAPGATRAAGVYIMIVDDKVFLFTDATVNIDPTAEDLAEIASLAADFAVKLEIDPH
                     VAFLSFSNFGSTPHPLSDKVRKAVELTKKRRPDLRVDGEMQADTAVVPEISEERYPFS
                     AVKDANVLVFPSLESANIAYKLLSRLGNAKAIGPILLGMGAPIHVLQTGDDVNAIVQM
                     AAVAVMDVMGREANG"
     gene            complement(20376..20792)
                     /locus_tag="PL000_04845"
     CDS             complement(20376..20792)
                     /locus_tag="PL000_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88255.1"
                     /translation="MNSNDIFVIEWQDPRTKTVLSVKYLKPWQWSDVVNALKEAEHYF
                     KSIDHKAIIIHDQSKFPPEQSTIGAVKDLWGKLPLPPANLRACFVVNQSKNYMLEVAF
                     DIVEKAFFRRTVTHFVYTMGEVEKMLEAFDLNSRNR"
     gene            complement(20896..21303)
                     /locus_tag="PL000_04850"
     CDS             complement(20896..21303)
                     /locus_tag="PL000_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88256.1"
                     /translation="MKNGPIIVYVEDDAPSIVVMKAIMNHVLGIVDNLIVIENTSDFL
                     EKVREIGSIPDIFLLDIHIQPYDGFELLKMIRSDNRLRYCKVVALTASVMNEEVEKLR
                     ESGFDGAISKPLDRYAFPKLIEKILDDQSVWHI"
     gene            complement(21306..23684)
                     /locus_tag="PL000_04855"
     CDS             complement(21306..23684)
                     /locus_tag="PL000_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88257.1"
                     /translation="MKLNKLSVRELLLLGFMAILGFNLLGMGISLINFNSFQSALKAT
                     SQESSQIRELSLEIQTEFLLARQDESAFLANWRLLGFDNARKEYGEQIQFHLDAARTH
                     LNELQTLSINSKNKNFREIANSTVQLSALLGDYENSFNATLGKIQTRIGANGLEKSLA
                     SGIEEMQFETRGLDNQFFFDTLIQIQKNEQGYFSTYRQEYIDYVRINTIELVDLTQAT
                     EEGGLETLNAEQLSTNLLEHYDTFTKIVALDQDIIKNTTIFRDITIDINLITKTIGEV
                     SRNGLILANQELDSTYRTTLLIQLTAGSLSLLSVIGVFIILARQTLRPLNQLTLAAER
                     LGQGDFEKNLDIHGLKEFSTLSQTFNSMSAQLSDLIESLEQHVAERTRDLQMATDVSR
                     QITQELKLEKLLPNLVKQTREGFDLYSTSIFLYQPETDELQLEADDVKENGVKTEPKS
                     FHINTRPSLTAEVARTRKTEVLNNIDQSSFVPTSRLPSIKSHAAFPMIVGDDLVGVLD
                     LQAETIGRFKDNDIRIFSTLAEQIAIAVRNAQLYRNQEKVTEELKRTDIMKSQFLASM
                     SHELRTPMNAIINLIDLVASEMVGPINTEQQAFLNQSLRSSHHLLHLINDVLDISKIQ
                     AGQLTLNLEQDVNIYEEIDTALKMSTPLIKESVEVIQDIDSDIPLIEADRRRIRQVLL
                     NLLSNAAKFTDKGSITLILKKRADYLLFAVADTGPGIPITAQTIIFEPFTQTEDGRKR
                     VEGTGLGLPISKNLVEAHGGKLWVESQPNEGSVFYFSLPYPQIPTYPILSQR"
     gene            complement(23723..24817)
                     /locus_tag="PL000_04860"
     CDS             complement(23723..24817)
                     /locus_tag="PL000_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC88258.1"
                     /translation="MSTKILKPVLCLSILTLMALLSACQGWSQQQTPEAKVSPEEINF
                     YNWDNYMDPNILSNFEKEYNIKVNYGTYTSSRDLRKRLKENPEAFDLFIDSDYAVSIL
                     QWDGLLAPLNKANIPNMKNLDPLFVSPQYDPDNRYCVPYQWGTTAIAYNRKATGRDLD
                     SWGDLFDPQFKGRVSFVESNRTAVGGVLLYLGFSPNTTNESEIYQARDYLIEHIDQFY
                     TLRGDDGQDLLVNGIVDVAVEYSGDVMQIAAENPDIKYVIPKEGAVLWTDNMCISVAS
                     PHKEQTEKLINYMMDPHIGAQLSNYIRYASPNQAALPFIEQSDLTNPIIYPPTNIRYR
                     LFYVVDVGQEANNIYDAAFEMILAAHDALK"
     assembly_gap    24955..25054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25055..26725)
                     /locus_tag="PL000_04865"
     CDS             complement(<25055..26725)
                     /locus_tag="PL000_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNC88259.1"
                     /translation="MNASSLPTLPANSIGYQYLKWVSLLMVGFTPFVTILPIYIYSQS
                     PSWQLLCFFTAGMVYGVGGWLSYRQAANNNHYIALRVITTFYWFAPGLGCFFLSEMPL
                     LMLTAAIYHIILFITLYPERLPFRNILIHLGIYLSLCFTAIFLDFSVAIPKWGFWIIG
                     GTVIASDIAYSIPFFLNFKQIQLAGKILVVQIQLTLIGFTAFAFIANIPEELHMILLT
                     IEGVIWLTTVWVLRYLTTPLIQLTENAEMIKQGDFSRRTKIEMNDEVGQLGIAFNQMV
                     EQLYNTLNGLESIVASRTRDLNVASQVSRDITRELNLENLLPNLVEKTRVAFNLYAVS
                     LFLFNPQTQELLFEAGSGASGEHMKGQAKAFHISAQPSLVSKSAREQKAEIVNDTSRS
                     DSHFFNPLLPNTRAEAVFPMVVGEELVGVLDLQSEYPNRFAESDIQIFSTLAEQIAIA
                     IRNAQLYRNQEKVAEELKRTDLMKSQFLASMSHELRTPMNAIINLVDMVASEMIGAIN
                     EEQKTFLNQSMQSSRHLLHLINDVLDISKIQAGQLTLYIEQDVNIYEEI"
     gene            complement(26764..27186)
                     /locus_tag="PL000_04870"
     CDS             complement(26764..27186)
                     /locus_tag="PL000_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88260.1"
                     /translation="MENTNLPLLNTRIFVVEDDVLNLSVISRPLSVSGASVFYNYNSI
                     GISLHIEQHLPIDIILLDIMLKRGVNGYDTFKEIKQNPKTAHIPVIAITSLDPETEIP
                     KAKAMGFSGFISKPIRVHGFAQHIADIIAGQKKWISSQ"
     gene            complement(27282..27914)
                     /locus_tag="PL000_04875"
     CDS             complement(27282..27914)
                     /locus_tag="PL000_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88261.1"
                     /translation="MSPNTIRVVLVDDHDYIHRIVTTILNQTTDIKLVGHAKNGEEAI
                     WVCEKLHPDVVLMDVLMPGINGLEATKALQAKMPNSKILILTSLQDQDMLFEMLRSGA
                     AGYVLKTTLKDDLAETINMIHRGKMVFSPEAFKTTPSDREQTGSSLGLTKRELDVLRA
                     MADGMNMPETAARLKISATTAKFHMENICGKLGVRTRSQALIIAVKLKLI"
     gene            28212..29456
                     /locus_tag="PL000_04880"
     CDS             28212..29456
                     /locus_tag="PL000_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88262.1"
                     /translation="MTNLESIARKITSILFAQQSLASAGFIAAATLNSIVGKELSGHS
                     DWAGVPSATYLLAGAFAAFMWGYVYDMIGRRNGLTIGLGIGAVGSGIAFYAIAIHSFA
                     IFLGGMVLMGIANAAVQLGRFAAAEVNPPEHRGRAISNVVIGGTVGSVIGPFVAGPAG
                     DLIGSWGIDELAGAYLVSLALFVIAAIVVFIGLRPDPREIGKQVAEKYPDTIVRSRVV
                     RSMMQIFSQPAALVALTSMVLGQMVMVLVMVITSLHMRGHHHDLTDISLVISSHTFGM
                     YAFSIISGRLSDQWGRGPVIITGSLTLVIACIAATISPDVLPLGVALFLLGLGWNFCF
                     VGGSTLLADQLSPDERARTQGFNDLLVGLASAIGSLSSGFIFAALGYNPMALISAIFA
                     LIPLLLASFWTLKRARKPRAIA"
     gene            complement(29518..30228)
                     /locus_tag="PL000_04885"
     CDS             complement(29518..30228)
                     /locus_tag="PL000_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88263.1"
                     /translation="MSMEDILKVLINSRQQGNSAQEADPMTSLVGSLLGGGQSQGGIN
                     LSDGVDAGDVAGLVGSLLGAQQAQNTPSQPQTQGLGGMMGALEGIMGGGQQANDPIMA
                     MLQPFIEPLAKKVNISPEIAMVIVSFVAHKLLAHHPTSGRDSNTFDLDEMLGQMGAGQ
                     VDSSLLHNSGMVKEISKRTGLDEATAEKSLNAAFTLVGNRVGLGNASPKPKMPGNSQP
                     NPSGPKTGMNSKSKSGKK"
     gene            complement(30297..30599)
                     /locus_tag="PL000_04890"
     CDS             complement(30297..30599)
                     /locus_tag="PL000_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC88264.1"
                     /translation="MPVYTYRCEACGVQFERHQSFTDTPLKTCPECRKKALKKVITPT
                     KIIFKGSGFYATDHKSPSGESSSTSSTKKTSKKEKDSAPKESASTESKSPSGGDSE"
     gene            30799..30987
                     /locus_tag="PL000_04895"
                     /pseudo
     CDS             30799..30987
                     /locus_tag="PL000_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            31026..32012
                     /gene="trpS"
                     /locus_tag="PL000_04900"
     CDS             31026..32012
                     /gene="trpS"
                     /locus_tag="PL000_04900"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC88265.1"
                     /translation="MAKKGRVFSGARPTGRQHLGNYLGAIKNYVAMQADYECVYCIVD
                     VHALTTVETTQELKQNTFEMALDWLAAGIRPEDSILFVQSHVPEVMELHTYLSMVTPL
                     GKLTDLPTFKEKVRQQPENINYGLVGYPVLMTADIVLYKTDVVPVGIDQAPHIEFARE
                     IVRSFNYRYKTKALVEPQVKHTEVLKVVGLDGKDKMGKSLNNHIELASTPEETVKRVR
                     EMVTDPARQRKTDPGNPDVCNVFTMHKLFSAQEEVDMINTECRKAGIGCVDCKLRFAG
                     NLNKHLEPFRAKRAELASNPNHIQEILNDGAKRARTIAQQTMAEVREAMQLP"
     gene            32092..32529
                     /gene="dtd"
                     /locus_tag="PL000_04905"
     CDS             32092..32529
                     /gene="dtd"
                     /locus_tag="PL000_04905"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNC88266.1"
                     /translation="MRLLIQRVSQASVTVEEQTISKIGKGLLILLGIGHGDGEEQAAF
                     LAEKTANLRIFEDDQGKTNLSVLDVKGEAIVVSQFTLYADARKGRRPSFIDAALPDVA
                     APLVERFVELLRSHGVPTQTGRFGAHMDVEIHNDGPVTIWLEK"
     gene            32526..33014
                     /locus_tag="PL000_04910"
     CDS             32526..33014
                     /locus_tag="PL000_04910"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNC88267.1"
                     /translation="MTIKPNFFQKFVHRFLMMRWVSAFLAVSLHRLDAIVLKLSRGRH
                     TVTELVGLPIIQITTIGAKSGEPRTMPLVSLFDGEKIALIGSNFGRQHNPGWYYNLKA
                     NPQCVVQTHGREQKFIARQAVGEEREKYWQMAVSYYKGYELYKVRAAHRQIPVMVLEP
                     MK"
     gene            33188..33397
                     /locus_tag="PL000_04915"
     CDS             33188..33397
                     /locus_tag="PL000_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC88268.1"
                     /translation="MSDRVIGTVKWFNGTKGFGFIEREGGPDVFVHFSAIRGEGFKNL
                     QEGQKVEFTIEKGPKGLQAADVVVL"
     gene            complement(33533..>34300)
                     /locus_tag="PL000_04920"
     CDS             complement(33533..>34300)
                     /locus_tag="PL000_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNC88269.1"
                     /translation="MKRDIYFSKPIINAAGSLGFSPDPRAGIPFDRFGAFITNPISLR
                     PRLPTPQPALIEFPGGFLLHTGLPNPGLPAVIKKHSARWNRADLPIIPHLMADRPEET
                     AQMVRMLEGIENVMAVELGFAPLLSNDILLLTLDMCLGEVPLIFSLPMEQVLSLGSKV
                     IEKGAVAISIACPRGALVASRDKNKLTTGRVYGPSIFPQALNTVRAAAMSGIPIIGAG
                     GVWTEKDAADMLSAGAMAVEMDASLWSPKEAGAAPQI"
     assembly_gap    34301..34400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34401..35567
                     /locus_tag="PL000_04925"
     CDS             <34401..35567
                     /locus_tag="PL000_04925"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNC88270.1"
                     /translation="GVFMQPLQGIRVLDLSRVLAGPYCTMVLGDLGAEVIKVEPPEGD
                     ETRAWGPPFAEGESAYYLCVNRNKRDIVINLKTDEGKKVLRDLALQSDVLVENFRPGT
                     LEKFGLDFATLSQSNPKLIYCSITGFGQTGPMRDKPGYDFMIQALGGLMSITGEPEGE
                     PMKTGVAVVDLFAGQNAVIAILAALQARTLTGRGQQLDIALFDSQLGWLANVASNYLI
                     SGKLPKRHGNAHPNIVPYQSFQASDGWFAIAVGNDRQFGRLCEVIGRLDLANDERFAK
                     NSARVQNREELIGLLKSIFLMRPVSEWLSALDEAEIPCGPINTFEQVFSMPQVQEREM
                     LVKMDHPTIGELPLVGSPLKFSDTPVQYRLPPPLMGEHTEEVLRELRDRGTKGE"
     gene            35600..35857
                     /locus_tag="PL000_04930"
     CDS             35600..35857
                     /locus_tag="PL000_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88271.1"
                     /translation="MKWLIPFFLILLGVSIFCCGFMYDAVFAGIPYQDPTPELVAKYN
                     FHSEVASVIRWGGAGTCAFGVVVAIGLALAGIIRPRRQKNT"
     gene            complement(36083..36667)
                     /locus_tag="PL000_04935"
     CDS             complement(36083..36667)
                     /locus_tag="PL000_04935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC88272.1"
                     /translation="MKLSTPEFRAMQSLPRKLGQRFFEMPVFRHMGLDLQNKDVLEIG
                     CGSGYGADLLNQLNPKSYIGLDVMEEQIELARVKYPQFEFLLQDATDLSQFSTTSKDV
                     VIIFGVLHHIPDWRKAIDEIVRVLKPGGSLFLEEPRGVDIKAFDFLFRWGHPDTDFGL
                     KVLDEHLTKRGLTILRKQWSPLLTMYHIKKRNDP"
     gene            complement(36709..38019)
                     /locus_tag="PL000_04940"
     CDS             complement(36709..38019)
                     /locus_tag="PL000_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC88273.1"
                     /translation="MSNKFFVAVIGAGPAGLFGARELATLGAHVAVFNRDVKAGGLAE
                     YGIYPDKHTMKEGLRKQFRQVLDLANIDYYGNVTIGANGDLTLDELRALGFDAVLVTA
                     GAQGTKSLGLPGENLEGVYHAKDVVFSYNKLPPFSQKKFHFGKRCAIIGAGNVMVDIA
                     RHLLHVQNVDEVTAIVRRGPAEVNFTKEEMKHLISYLDLDDFNREMERVRPAVEAVKQ
                     DLETGRQKILDSLAKADPKTAHGKFRFDFLASPTAMYGENGLLTKLEVEDNILTERDG
                     KTSAKGTGAKRTLDVDTVIFAIGDKVDESFGLPTEWNEFIKNNSPRFPVDNLTFESAV
                     EGIFVGGWSRKASEGLVGYARKDGTNAAKAVWQYLQTKQPANANAEVVAEKMKNLGKP
                     IILKEDIKRLEAVEAEEARKRGLEEFKFASNEEMLQAMGFMETA"
     gene            38150..38986
                     /locus_tag="PL000_04945"
     CDS             38150..38986
                     /locus_tag="PL000_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88274.1"
                     /translation="MAEDNKKDPAQKMRYLLNGGKPSPVARLPKKDGGVASFLPKLKK
                     DDAPTETPAAVEVDVAPNKDQPEAAHQTPPKPAPFQGALSSLKGIKLNIGPAFWTIAS
                     VISMTVNVVLLIVLLALLPNLTKLNLGSALDIGNYLLGGLYSNFEKMDRAHITTSIPV
                     ETTIPVKFDLQLNQQTNVVLSQDVTITNALVTVNTGGLNITRALTTIVLPQGTNLPVV
                     LNLVVPVDTTVPVVLNVPVDIPLSQTQLHEPFAGLQDVVRPFYCMLSPNALGIDGQPV
                     CK"
     gene            39054..40319
                     /locus_tag="PL000_04950"
     CDS             39054..40319
                     /locus_tag="PL000_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC88275.1"
                     /translation="MTNQITQVTLKNGMKVMLKEIHTAPIISSWIWYRVGSRDEPTGK
                     TGISHWTEHMQFKGTKKFPGNVLDKAISREGGRWNASTSRDSTRYFETMPAAKIDLAL
                     RLESDRMKNSLFDEKEVASERTVIMSEREGLENEPTFRLSEAVQQTAFHVHPYHHEII
                     GDMADLRTITRDDLYEHYRRYYVPNNSILTIAGDFQTKAMLKRIQELFESIPMGKTPP
                     RLNRPEPEPKGEVRLTVEGPGETTFIQVAYRFPNAAHPDYFPLQVLDSLLGGASGLSY
                     KTSRLYRALVDKEFAVDVSSWPEATIDPFLYRIIVTNHPKRKTDQTLAALDAEIQKLQ
                     EKRVSKDEITRAVKQARAVFAYGSENITNQAFWMGYAEIFSSYRWFTSHLSNLAKVTP
                     QDVQRVAQKYFQPANRVIGIYTPNGRRSK"
     gene            40316..41602
                     /locus_tag="PL000_04955"
     CDS             40316..41602
                     /locus_tag="PL000_04955"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC88276.1"
                     /translation="MTKPVSVKKKSSLPGPEDIHRETLPNGITVLARSNFNSPSISMA
                     GYLPAGAIFETDDKLGLADFVSASLMRGTQTKSFDAIYNELESVGAGLGFDSGVHNAS
                     FGGRSLVDDLPLMLKLLSESLRTPVFPKNEIEKLRAQILTGLAIREQDTSDMAAMAFD
                     RLLFDGHPYSRPSDGFVETIQSITQKDLVNFHRDHYGPRGMVIAIVGAIDPKKAVAAV
                     ERVLGGWQVPGQVVPPLLPPYKPIKKTVRHHHTLANKSQSDLVIGMIGPKRKDPEFYS
                     ASLGNSVLGQFGMMGRIGESVREKSGLAYHASSSLSAGVGPGTWEVNAGVSPKNLKKA
                     IDLIEEELRKFVKSGVTKRELEDSQANYIGRLPLSVESNGGVANALLNIERYDLGLDY
                     YQRYEGLVRGVTRDQVLATAQKYIDPNRLVISTAGP"
     gene            41615..41980
                     /gene="acpS"
                     /locus_tag="PL000_04960"
     CDS             41615..41980
                     /gene="acpS"
                     /locus_tag="PL000_04960"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNC88277.1"
                     /translation="MKLATGVDLIEVSRVREAIERHGERFLARIFTEIEQRECGGKFP
                     SLAARFAVKEATAKALGTGIGDVSWLDIEVRGDENRAPHLYLHGAGEELAQKLGLVTW
                     SVSISHTETQAIAFVVAIG"
     gene            complement(42074..42298)
                     /locus_tag="PL000_04965"
     CDS             complement(42074..42298)
                     /locus_tag="PL000_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88278.1"
                     /translation="MLWFDNDPRTTLSIKIQKAMDFYRKKFGHIPDLCLVHPSMMENG
                     QRQVELGKLIIRPYKPVMPGHLWIGVEDQK"
     gene            complement(42343..43134)
                     /locus_tag="PL000_04970"
     CDS             complement(42343..43134)
                     /locus_tag="PL000_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88279.1"
                     /translation="MDNSNHFTLMINALTELAKEEQSGHTSRMPEIILPTLNSVLADI
                     TPLPEEALFLGIAEDGLPVLLNLDDPIPGPILIVGDKACGKTGLLQTIAQAAELLHSP
                     SDIQYCVVTSHAEEWNNFQDRESNMGVYTTQDNGAQELIQSLVEWAHQNKNDERTFLL
                     LIDDLEFLTKLDQQTEQCLRWLLLRGTSRKVWPIVTLNASRAHHIETWLGFFRTRLFG
                     NIQNLQDAHLVANASNDILGTLATGSQFAMREGQNWLRFLAPALD"
     gene            43276..43348
                     /locus_tag="PL000_04975"
     tRNA            43276..43348
                     /locus_tag="PL000_04975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43309..43311,aa:Thr,seq:tgt)
     gene            43395..43477
                     /locus_tag="PL000_04980"
     tRNA            43395..43477
                     /locus_tag="PL000_04980"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43429..43431,aa:Tyr,seq:gta)
     gene            43511..43583
                     /locus_tag="PL000_04985"
     tRNA            43511..43583
                     /locus_tag="PL000_04985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43544..43546,aa:Thr,seq:ggt)
BASE COUNT        10202 a         9935 c        11363 g        11984 t
ORIGIN      
        1 tgtggatttc atgcccatgg catgccccaa ttcacgtgct gcaacctgca aggcatcttc
       61 cacagtggtg gcgttttgaa ttttctgggt aatgatgttt accagggtct cacgttccgc
      121 ctgtcgcttg gattggatga gggtgcgggc gttctgcacg gtcacagcga tctgagaggc
      181 aagcgtggtt tggatgttgg catcttcttc ggtgaaggca ttgaggcggt ccgattgtac
      241 atccagtacg ccgatgagtt gatcgcccac cagcaatgga acagcgagct cggatgcggt
      301 atctgggagc agcgggttgg gcagccagcc cggttcattg tgaacgtcat tgatgatgac
      361 ggcttgtttt gtgcgggcgg cacgtgctac aagggattgt tcctgcaata atggaataat
      421 ggttgtgccg tgtgtgccct catgttcatc gccttccttc caaccgcagg caatgatttg
      481 aaggtggttt gcgttcctgt cgtacaggaa gacgtgggca tggtacaacc caaagcgtcg
      541 ttgggtcaga tacaccacat tttccaacat acggttgata tccgcttcac gggatgagac
      601 ggtactgatc tcagccaccg aggcgagttc cttggcgcgt ttggtcatgg tttccaccga
      661 gccgatgttc tgcaaggcga tcgcaacctg gttggcaatg gactgcagtg agtcaacatc
      721 ttcacgtttc aggccatcta cgatgttgtg ctgaatatcc aaaacgccca acactttatt
      781 accgatggaa ataggcatgg ctacttctga ttttgtctct ggcaaaagcg gattgggcag
      841 ccagtttgga tcatggctgg tgtcatttac caggatcggt tcattgcttt cggcggcacg
      901 accgacaaga ccctgacctt tggaaagttt atgtccattg gccagcatgg ttgcacccgc
      961 ttcacctgta ccacccgcca tgaccaattc gccatgttcc tcatcgtaca tgtaaatgtg
     1021 ggcatgatag taattgaagg agttttttac ctgctccaca acttctgtta ccaactgcct
     1081 ttgatcgaga attgtagaaa gacggcggct gacttcggaa gaggtggcca gcgcttttgt
     1141 acggtctgca acgcgttgtt caagagtcgt gatcaactcg cgtaaacggg atgtcatgga
     1201 gttaaaagag tttgccaata caccgatctc atcctgttgg ataatatcca catgttgatc
     1261 cagattccct ttggcgatgt tttcagcggc ggtggccagc tttgttaccg gggccgtaat
     1321 gctttgagtg gtgaggaagg ccgcaaaacc tgcagcaaga actgtgatca cgatcaaccc
     1381 aagggtgact tggaacaccc tgctggcagc gctcaacgct tcgctttcat cctgttcagc
     1441 gatgattgcc acttgaagct caggtaccca gtgatataca cctgccactg ccgtattccg
     1501 gtagtctgga taactgcccg agccattggc cttgcttttg atcgcgtttg taactccatc
     1561 ggtatgtatg taggtttgac ccagaacggt ctcaccaaaa cgcaatttgg tcagcacagc
     1621 gaaattggaa ctgaccatat aggtttcacc agtttctcca aggcctgtgc gttgctgcat
     1681 gatttcatca agcgtattta agttgacccg ccctgccact acaccaatgg tttttccagt
     1741 attgctgaca agcggttggg atatgacgat agtgtagttg gagagcgaga gttcataaga
     1801 aggcggtgtg atatatcgcc cctttatacc ctcatgaaag aaggattgtg tcgtcaggat
     1861 ctttccttcc tgattgcttt cagtggaaac aatgacctgt ccatccttat ccatcacaaa
     1921 gatttcagtg aaataccctg aacgctggct gttattcgtc aactctgttc gcaccagatc
     1981 tttctgttgt tcatcgggct cttgattaag cacaagttcc gaaacgcgtt ttagcgaatc
     2041 cacattgaca aaaaccatgt caaggttggt ttgcagagat gccagccaat catgaacctg
     2101 agactccttc agagtgacaa ccgattccaa ttggtcgaaa acgtctgctg tcagaccctg
     2161 agaactgatg taggtagcca cagcaccggt aatggatacc gggagcagca ccagcaggat
     2221 ggatgtgaca agcagacgcg tacgtagcga tgtgaatcgg atcgaccaac gcggacggct
     2281 gacgcgtttt aattgagtgt tggtttgatt catgagcgcc ctccggcaag tgagcaaaag
     2341 acagagctat atattgtgtt gacgaatttt aagcctatca tacggtatat cttcacgagc
     2401 atgccttgca ttgtgcgtac aagtttttac tgaccgacaa actgatttgt gtacactgtg
     2461 tttagttcga tgggttcggt gatcaggttg aagtccagca ggatttgctg ggtggtttcc
     2521 cagacatcac catccattgc gccgatgggg gcgtcacccg tatcaataat ggggatttgg
     2581 gcggtcatca cattgacttg aaatgcgaga tccagggtcg ggtcatattt caaggcgaat
     2641 tcagcggctt tgtcggggtt ttcaatggag aattgataac ctttcatggt ggcccgcacc
     2701 attctggtga tcaggtcgga attgttttga agaaattcct cagacgtgaa aagtacgttt
     2761 gcgtacacat ccaccccgta atccttgtaa tagatcaggc tgacatcatt cccggccatc
     2821 gtggcctgaa tgggttcatt ggtggcgaat gagccgctca tggcatccat gccgcctgtg
     2881 atgagttcgc tggcaccgtt gtaatcgtca atggcttcaa aggtcatact gttttgatca
     2941 atgttcaggg tgcgcaataa agccaggaat tgaatgtcat aggttgtgct taaatccggg
     3001 gcaatgacgc ctactttttt gccagccagg tcttcaggct tgttgatgga gcttgaatcc
     3061 aaggtcatta caaccagtgg gttttgtctg aaaatggtgg caaccgcaac gtagttctgg
     3121 ttgttggttc tggcgatgat gaggctatcc cctgtggtga tgccaaattg tgactttccg
     3181 ctttccactt cagccagtgg attgacttcc ggtccgccgg ggatgagggt tacttcgatg
     3241 ttttcatctt tgaaataacc attttcaatg gcggcgtaaa accctgcgta ttccacactg
     3301 gggaaccagc ttaattgcac ggtcacttta tcgggggccg aaaagttcat gttgccgcac
     3361 ccagacaagg cgagggagag aatcgtaatc caggttgtga agacggctaa tttcgaaaat
     3421 cgatttaatg ttttcatgat cgcgtgctcc tttggtgtta aagtcttgca agatggttat
     3481 gaaaggggta acgacaaggt gaaactggtc cctttatcta cttcgctttc cacgttgacc
     3541 tgtccgtggt gatgctcgac aatggatttg acgatcgcca gaccaagacc gttgccttcc
     3601 acttcgatat tgtgtccgct gcgtacgcgg tagaaacgat caaagataaa tggcaggtct
     3661 gcggcaggga ttccgcagcc tgtatctttc acctcgatca aaagctgcga atcactggcc
     3721 tgggttttga aagtgatttc cccatcggtg ggggtgtatt tgatggcatt tccaagcagg
     3781 ttgcgcagca attgacgcaa ttgcaaggga tcgccactta tggtgacggg ttgctcaaga
     3841 ggggtgtagg aaaaccgctg gtttttgata tttgcctgcg gtatgaactc gttgaccacc
     3901 tcggtcatta ccttgtccat gtccactact tcgtgctttt gggtcgcatc caggtccatt
     3961 tgtgcgagcg acatcatgtt ttgcaccagt tcgctcatgg tgtgggtggc ggtttggatg
     4021 cgggttacaa agtctttttg ctgctgattg agcggccctg catgttcgag cagggtgctg
     4081 aacccggcaa tggtcgtgat cggatttttt aggtcgtgtg aggctgtggc gatgaattcg
     4141 ttcttgacct gttcaaggtc tttgaagcgg gttacatcgt gcagaatggc aacctgcccg
     4201 ccatcttcga tgggggtgat gagggcattg aaggtgcgtt ggtcgggcca ggtaatatcg
     4261 cctgattttg ctccatgcgt cgtgcgcgcg tcatccagca tatggatgaa gacatcgtag
     4321 ccgtggtcat gggggagggg ctggttgagg ttggtgctga aatcggtgaa cagcttttcg
     4381 ccttcagggt tgagcaggga aagcttgccc tgtgcatcga acaccagaat ggcttcggcg
     4441 gcatgcttca gaaccgccga aaggcgattc tgttcctgca tggcaatgga gtacaggcgg
     4501 gcattttcaa tggcgatggc agcctgactg gtaatcgcct gcaaaagcag taaatgatcg
     4561 gtgttgaaat aatttacctg ttcgtgggtg aggacaagca ggccgagcag ttccatacgc
     4621 ccgaacagcg gactgatcac cgcagaatgg atgtttgagt cttggttatc aagctgccag
     4681 agtgaattat tgcgtgcatc ggcaatgatc agaccctgat gcgattcctc gacatgtttg
     4741 atgatgtttt tgggaattgc gatcatgctg tttgcagaat ctggtgaggc ggcaggcacc
     4801 tgataatttt gtgcaggacc gccgttggtg ccgaagatga tcatgttgcc ctggattgcc
     4861 cctaacgttt ccacggtgcg cttgatcagg atgttggcaa ggtctttaat atcggtgcgg
     4921 gcgctcaatt ccttgccgat ctcgggcagc aggttcaatt cgcggttgcg gcggcggatg
     4981 atgtcttccg tctctttcac acgcagtttg gtgcgaatgc gtgccatcag ttcatggcgg
     5041 tcgaaaggct tggtcacata atcgtctgcg ccgaggttca aacccgattg gacatctgaa
     5101 ggatcgaggc gggctgcggt gaggatgatg accgggatat gctgcaccga aggatcactg
     5161 cgaatctctt cgaggacggc aaagccatcc ttgtgcggca tattcacatc caaaaggacc
     5221 agatcgggca attcggcccg gactttgtcc aaagtatcca gcccatcgct tgcggcaatg
     5281 tgctcgtacc cttcgtattc cagataacgc gtcaacaggg tgacattgtc cggcctgtca
     5341 tctgccacaa ggatcttgaa cttcttccgc agcaccgacg attgtttaat ggggtgcgtg
     5401 gaatgtttca tttcctcgat ggcatttgtg gcgatctggc aaatgcgttc aggacgcacc
     5461 ggcttgacga tcacttcatt gaccttcaag cggtgagcgg tgaccttcag ccctggcacg
     5521 tcatatgctg tgacgagata agtgtatgat ggcctgccag cagggtggtt ttgcattttc
     5581 tcaatgagtt caagcccggt catttcgggc atgatcatat cggtgaacaa aatatccacg
     5641 ccgttggttt ttactttttc cagggcgtcg tgcccgctcg aggctgagat gacactcaca
     5701 cccgtaccaa gctgggaaag cgcgcgagcc agcgtggttg cggtgttcgg gtggtcatca
     5761 acaacgagga tgcggacagg gtttgtactg gtgggggtgg tttccggagg tatcatccat
     5821 ggcattttcg ttcaaaattg cacattcaac gatagtcatc tggatgattt ggttggggaa
     5881 agttggcaac cctgattcgg ggagattcgg tctgggaatc aaaaacccgc ttccggctaa
     5941 gctggaagcg ggtttaaatc caattaacag ggtcggcgcc gatactttaa tctgccacca
     6001 gacggtagcc cgtacctcgt atgttgatca ggtaggttgg ggaatggatg tcgggctcaa
     6061 tggcctgacg cagttgatga atgtgccact tgctgagctc ttgcgcttca tggagatcgg
     6121 cctgataccc ctgggcttcg gtcacaaggg tttgataact gacgacgtca ggggagtgcc
     6181 ttgccaacac cagcaggtat tcaaacgaag tgggcggcag gttgactgcg tgttcgttga
     6241 tgttgaggca gcgtttatgc aggtcgagca ccagtgtgcc gcgtttgatg tagcggttcg
     6301 catctacacg ggtggctggg agcgggccag tatcgccatt attgaggcct tttagttcgg
     6361 cttgcaagtt ttcgatctgt tgcatgatct cgagcttgcg tttttctttt tggcggctgg
     6421 cgatgatttt ctgggcgttt tcgatgacga tctgcggttt gagcggtttt agcaggtagt
     6481 ctgtggctcc gctgcgcagg gcttctacgg ctgaatttag atcaggttgg gcggtaaaga
     6541 gtaccaccgg caaggttggg tagttggcat ggatgagttt gagggcatcc agacctgcaa
     6601 tgcccggcat ccgcaagtcc atgaagacga ggtcgaagag ggtggtttcg atgaaggcga
     6661 gtccctggtc agcgttttcg gcggtggtgg cttcgaaacc cgcttgctgc aaaatgcgtg
     6721 ccaggctttg gcgcagggtg gcttcgtcgt caatgatgag gatgtgcccg ttgctcatag
     6781 aatttctcca ctggtgacgg gcagccagac acggaaggtt gcgccctgat cgtggttgtt
     6841 ttcggcggtg atacgcccac ggtgtttcat gatgatgtca taggtgatgg tgaggccgag
     6901 acctgtgccg ctctgtttat cggtgacaaa ggcttcgaag atggtgggca atatcttggg
     6961 tggaatgccc ggaccattat ccgacatgat catgacaact tccccggtgt ctttttggta
     7021 ttcggtgcag actttgatcc tgccgccatt ttgcatggct tcactggcat tcatcaatag
     7081 attaagcacg acctgacgta tctggtctgc aagcgctggt atgaacggga ttgcagggtc
     7141 gggggtgaat tcgaagctga tctggttgtg gcgaagatgc gtggagacga gggcgtacac
     7201 gtcgttcaca atgtcgttga gttgggtggg ctgaaaatct tccgcgtggg tgggacggta
     7261 ggtgtcacgc aggcgtgcga tcatgttggc catgcgttcc gattctgcaa ggacgatgtc
     7321 gaggtcttgt ttgccttggg gcgagacgtt ggtctcctct ttgaggagga acagggcgtt
     7381 ttgaatggcc tgtaatgggt tgttcaactc gtgtgagacg gtagccagca ggcgtcccat
     7441 cactgtgaga cgttcatttt gcattaactg gttgcggatg gttttttcct gttgcaggga
     7501 tatttgcagg gtttcgtaca agtccgcttt ttgcagtgcc acggccagtt ggtcggctac
     7561 ggcgctgacc agttgcaggt ctctttgcga gaggtatttg ggcggtactt gatgaatatc
     7621 gagcaggcca aggatctgtt taccgacctt taccggcact gcaagttcag attttgtatc
     7681 tggcaggagc gggtggcgga caaaagacac cacatcatct acattattgg tgaaaaaggc
     7741 tgttcccgtt tctgcggtgt agccgacaat accctcgccg ggcgcgagtt gatgttcgcg
     7801 tgctttgagt aaggcgccaa ccttgccgct gcctgctcgc atgatgaaat tgcccgtatc
     7861 cgggttggca acataaattt gaacgtagtg atagccgaag tttttttgca ggagttcaac
     7921 tgtatcttgc aacaggtcgt gggggtcaag tgaggctacc aatcccttgt tgacacggta
     7981 cagggcgttg gcttccttga gggcctgttt tgtattttcc aacaggtttg cgttttcaat
     8041 tgcgatggcg gcttgtgtgc caagctggct gagcaatttc agatcgctgg gagtaaaggc
     8101 gtaggggcgg ctgctttgaa tgctgatggt gccaagcttt tgttcgccgc tgcgcaccgg
     8161 tgtgaccaac agggaacggt aggcgggttc cactccaagc cttagaaagc gcgggtctgt
     8221 gcggacatcg gcgatattga tcgtctcacc actgagaatg gcctgcccag ccaccccttc
     8281 tcccagtcgt atgtttctct ttccaccttc aagctctgtg tacccaacca cggcctctga
     8341 aacaaggatt tggtcctttt cgttcaatag gtggatgaca gcctgttcgg agtttgcgat
     8401 caagtccttg gcagatttta cgatcagatg caatacattg ctcaagccaa tgcgttcagt
     8461 ttcgctcaaa gccagagaaa tatccgccaa agattgcatt tcccgtaacc cgcgttgaag
     8521 ttcgttatcc tgttgtaaat tccaaatggc cagggcgatc tggttgccgg cttgttcggc
     8581 gcggttcaat tcctcttcgg tgaattgatg gggctccgca aaccccacaa tgactgcccc
     8641 aagtttgtga tcaccgtaat tcaaagggat gctgataaat gatttttcag gaaagaggtg
     8701 aataagggtt gcacttacat aggtggaatg atgcgggtct tccacaacga tgatgcggtt
     8761 ttcctgtaag gcggtgatgg tgaggttggc ttcccctttt ggatactcca tggtgaggaa
     8821 ggccgtgttt ttcaggttgt tggttgctac aggaaatacc tgctccttga ccgggtccca
     8881 acgggtgata tagcaactat cagcatccag cagggtggtg atgtcattcg tcagcgtgtt
     8941 tgccatgctt tcgaagttct gtgctgtggt gatggcatgg gccatgtcgt tcagtagttc
     9001 gagaaaacgc tcacgggagc gtaattgact taagatgctt cggtattctt cgtatttgta
     9061 tttgatccaa ccgacgctga tgcccagtag tgtgataaga atcgcaccaa gcccatagtc
     9121 atatccattg atctctgccc acggaatctt ctgaacgtta accaacaata ctgtgttgac
     9181 aagaatcgtc aacaagcttg caataattcc accgggaatc ccaaaaaaat aggcgctgac
     9241 caccaccggt aagccgctca acgtagtgat ggcaacgcca aagtagttaa acaacaaaat
     9301 ggaaccgatc aggtaaatta ctgctgaaag gccggtaaac agccattttg cctgagatga
     9361 ttccaagtat cgattcgttg ttggttttgc gttcgggagg gccataaact tggtttatta
     9421 taatgcgcct gtttggggcg gcacattgtg aaatccttgt aaatgaagcc tccacctttt
     9481 aggtgggagg cttgaaactt cccaatcaat caaaaagccc aaagaaatat cctttgggct
     9541 ttccattttt gttttgttta ttttgcgtat tcggttgccc gtgtttcgcg tatgaccgtg
     9601 accctgatct gaccgggata ttgcatggtc tcttcgatct tcttggcgat atcgcgtgcc
     9661 aatctggcgg atgagaggtc atcaatggta tctggtttga cgaggatgcg aacttcgcgc
     9721 cctgcctgaa tggcgaaggc ttgttgtaca ccctcaaaag acatggacat ttcttcaagg
     9781 gtgcggatgc gtttgatgta ggcttccaag tcttcacggc gggcaccggg gcgggcgcca
     9841 gaaatggcat cggctgcttc agcgatcaca gcttcgacag tttcctgatc cacctcatgg
     9901 tggtgggcgg cgatggcatt gaccacggtg gggtggacgc catagcgttt gcaatattct
     9961 gcgccgaggc ctgcatgggt accatcctgg ttatgatcca tggctttgcc aatatcatgc
    10021 aggaagccgc cctgtttggc gagctcgata ttcgcaccaa gctcggctgc gaggatgcct
    10081 gcaagtttgg aaacttccac ggcatgggct agctggttct gcccgtagga cgtgcggaat
    10141 ttcaggcggc ccatcatgcg gagaacttcg gtatgtaagc cggtgacatt ggcttcatag
    10201 gcggcttgct cgcctgcttc gttgatgatc ttttcaacgg ctttggtttc atcttccacg
    10261 atcttttcga tgtgggcggg atgaatacgg ccgtccaaaa taagcttgga aagggcgcgg
    10321 cggccaattt cacggcggac agagtcgaag caggagatgg tgaccgaatc aggtgtgtca
    10381 tccacgatca catctacacc ggcggcctgt tcgaaggcct tgatgttgcg tccattacgg
    10441 cccacgatgc ggcctttcat ttcctcgctg gggagggtga ccactgcacg ggttacttcg
    10501 gcgacatgtt cagatgccac acgttgaatt gcatcggcaa tgagtttgcg ggcgcgtttt
    10561 tcgccttctt cgcgtgcttc ggcttcgatc tggcggatga tgcgggccat atcaccgcgc
    10621 gcttcctttt caaccagggc aaacaattcc ttgcgggctt cgtcttgagt catctgtgcg
    10681 atgaactcaa gttttttcat ttggtcatcg taaagcttgt caatctcgtt tgcgcgtttg
    10741 tcaacctgtg actggcgctt gttcagcgtt tgttcgcgct gttccatttt gtcgaaacgg
    10801 ctatccagtt cgctgcggcg cttgtcaagg cgttcggttt ccttgtttag ctcgatgcgg
    10861 cgttccttga cctcagcttc ggcggctttg gtgatctttg cggcgttttc gcgtgcgccg
    10921 ctttcgatca ggcgggcctg tgagttggcc gcctttaaaa tgtcatctgc tttttcctgc
    10981 ctggctttcg cagcttgttc ggcttgatat cggtgaaaga aatatcctgc agaaacaccc
    11041 acgatgagca caattaggtc aataattagg acgagcgggc tcatgttatt tcctcatttt
    11101 catcgtcaaa aattttttgt tccccagttg gatgagtttc actccatgcc ctggtgacga
    11161 cgggggcgat gatggagtag gggaatcctc gtcttgcgag gaactcggat aatttttttc
    11221 taaagtcgtt ccattccaga cctttgagcc tgcccagcct tttttgggca gcttcgtaag
    11281 ccagggtttc ttcgtccact gctgatactg cggtttggac ggtttcgtca ttgaggcctt
    11341 tttggcgtag ttccattgcc agagcccgcc gacttcgtgg gcggaaggtg ctgcggtttt
    11401 ccacccacgc ctgtgcaaat tcatcgtcat tgaccagacc tgcatcacgg agacgttcaa
    11461 tggttgtttc aacaacgtct tcggaatatt cgtgcttgtg caggttctgt cgaatttcct
    11521 tctcagaacg ggcgcggtag ctcaaaaaga gcatggcttg ttgatatgcc cgttcacgtg
    11581 agtcttctga aagcaggctg gcgatctttt catcgctgag ttcgtcgcca actttcaacc
    11641 atgcggccgt gatacgcgcc actccaaagg cgaactctcc atcaagatgg atgtttaccc
    11701 ggttgggggt cttcttttga accgaaatgg cggttatctt tttcatttgt taaaaatcta
    11761 cagccaaggt ctccgaccag tggaaggtcg gccttggctg tggaaaatgt ctgggttgac
    11821 tgagcaggcc caagtccgtt tgaggcggat aaattgggat aggtcagagt acgtgagcaa
    11881 tcgtcacgtt tggcatgggt tgtggggggt gaagccagtc tagggtgttg gtaatattca
    11941 tttagaaaga cttcacaaaa taactcccaa acgcttgggg cgggggaggg atcgagcgcc
    12001 aaatttccag tcagatttca agcaaggccg cgaacggcgg agttttcaat gtcaaaccct
    12061 tacggatttc cttaattata catgaatttg aagatttgtt aacatttgga atccatatta
    12121 aatcacccgc tggccttgaa atgatgcctg ttttattgag tataataaaa tttcttccca
    12181 acctggttta tgaccatgtt ttggcctttt taactgaaag cagtttctca aatactggag
    12241 gaatcgatga ctgaacaaca acaagaagcg actgggggag atattgggag gatccgacgg
    12301 ttcattgctt ttctgggaat cctttttatt ttggtgagcc agtttttgat attctcccag
    12361 cctgttgatc atacgttggt gtttccgcct tatacgtggc tggcgattgt gggggtgctt
    12421 gttttggctt tgggtctttt tatccgccca acggcgttct ttcaacggat ttctgactgg
    12481 ttcgtttttc aagagcggac cttttgggtg ctggctgctt ttttgatgtc ggctttggtg
    12541 accatggcaa cggccaatct gatgttgttt gcccgtatta attacattcc cgtcctcacc
    12601 atttggttat taagcgcctg ggcttatatt tatgcctttt cgaatttttc cattagtgga
    12661 tctgcgatct ttgattggtt caaaaagaac cgcaatgaac ttttggctgt tttggctgta
    12721 accctgttgg ctgcaggact tcggttttac aggctgggtg gtattccgcg tattttggat
    12781 ggtgatgaag gtctgatcgg tcttgcggct caaaccacaa ccagcgggtt gctggcgaat
    12841 cccttttcgc tgtgggaaaa cttcggggcc atgtatctgc atttgatcaa taccgccatc
    12901 agtttttttg gggtgaatgc ctttgctttg cggttatggc ctgccattgg tggaacgctt
    12961 gccatcccgt ctttgtatct tttcgttcgt tggatcgggg ggcgaagaat tgctttgatc
    13021 accgccttga tcattgcttt ttcacattcc catattcatt tcagtcgaat tgtgtctgtt
    13081 gcgtatatcc acgggacgtg gcttgctccg ctggaactgt atctcatgct tagcgggttg
    13141 gaaaaacgcc aatcatggcg ggctgcgctg ggtggggtgt taattgccat tcatttttcc
    13201 atttatttga ccgcacaggt gatcattgct ctggcttttg tgtttatggt gttggcattt
    13261 ttgttttatc gaccctggtt caaggagcgt tttgctcagg caatggcttt ttggggtggt
    13321 tttttaatta tcatcctgcc ttctgctttt tatatttatc gaaatcccaa cgagtttttt
    13381 aaccgtctgg ctcagaacgg cacatttcaa tcgggctggc ttgagttgac catgcaatct
    13441 acggggcaaa gcgctgccga aattttattc ggccgctttg tacatgcctt cctttcacta
    13501 acttattacc ctgccattga tttctatggt tcatcctcgc cgatgatgag catgatttca
    13561 agcaccatgt tcctggtggg gttgctggtt gctctatggc gaattcgaaa tccagcctat
    13621 ttgcttttga acggttattt ttggggggct actttctcgg ttggtttgtt cgccattcca
    13681 cccaattcag attcctatcg tatgttgatg gcgcttccct cagcgttgac tatggctgcc
    13741 atggggcttg accagatcct tgaattgctg ggcctggggt ggaataaagc ccgcacctct
    13801 tacacacttt ccgcatcggc gattcttgtg agcctgctta ttttcaacct ctggacgtat
    13861 tatggcgact ttgcaggtaa atgccgcttt ggtggtgatc tggtggggcg atttgcttcc
    13921 taccttggca gttacgcaca cagcgtggat aacgaacttc ccatctattt gctgagtaat
    13981 gggatttatt tctacggcag ccacgcctca accgattttt tgagcgagag cagggtgatc
    14041 acaaactttc ctgacccgat tgatactttg cagcccgtct ctggagaaac aattatcgcc
    14101 aaccccgaac gcatccccga gttggaagca tgggcacgtc tgaaccctgg cggcgaattg
    14161 cattatcgct acgattgctc aaataccatc ctgttggctt atcgggttcc ctagaggaga
    14221 cttggatccg cgcgaatacg aacttatgta tcaagtcgaa gaccgtcact ggtggtatcg
    14281 tggaatggaa accatcacac gggctctgct tgaacgctgg gctccagcac gccctcaaag
    14341 aattttggat gcaggctgcg gtaccggcgc cgccatgaca acctacctca cagattatgg
    14401 aaaagtgacc ggtattgact tgtatgccga agccctgaaa ttttgccgcg aacgaaatgc
    14461 tccacgcctc gcacgcgcct ctgtgctgga cctgccattt gctcctgctt cctttgacat
    14521 ggtcaccagt ttcgatgttc tttacgagcg ggccgtctcc gatgaattga ccgccttgaa
    14581 ggaatttttc cgtatcctga ccccgaacgg gtgtgttctg cttcgcctgc ctgcctatga
    14641 ttggctgcgt ggacaacatg acagaaatgt gcataccaat cgccgctata caaagaaatt
    14701 ggtcaggcat ctgcttgaac aaagcggatt tacggttgaa catctttccc atgccaatac
    14761 atttcttttt ccccttgccg tgatgaaaag aatgggggag agggttttcc ctcccaaaga
    14821 caatcaatct gaccttgcca tgaatgcagg tgtattcaac gatgctttga agatcatcct
    14881 ttctgctgaa gcccctcttg tctcaagcat tggcctgcct tatgggttga gcgtgatcgc
    14941 aatcggtaga aaatgacaaa cctgatgaag catgaaagag aaagccagca acttcccagt
    15001 atcagtgcgg ttttcccagc atacaatgac ggcgggacaa ttgccagcat ggttgctgct
    15061 gcctggatcg ccttgaatca ggtcacttct gattacgaga tcatcgtcgt aaatgatggc
    15121 agttccgact atacagccac catgttggat gagatatcgg ttcgatatcc tgagctgcga
    15181 gtgattacac atccgtataa tcaaggctat ggcgcggcat tgcggactgg gttttccgcc
    15241 gcatccaaag attgggtttt ttacaccgat ggcgattcgc aatacaaccc gcttgaactt
    15301 gtgaatttgg ttggcgcttt gtacgaaggc gtggatgtgg tcaatggata caaactgacc
    15361 cgtaacgact catggatgcg gatcgtgatc gggcgggcct atcattacct cgtcaaattt
    15421 cttttcggca ttcatattcg cgatgtggat tgcgattttc gattgattcc ccgccgcatt
    15481 ctcgaagaga ttgaattgaa aagtgtcagc ggcgcgatct gcctcgaact ggttaaaaaa
    15541 attgaggatg cggggtatgt ctttgcggaa gttccagtca accattattc acgcaaatac
    15601 ggagtctcgc aattttttgt cccctggcgc attgcccgta ccctgtatca actggcggat
    15661 ttatattgga aactggtgat tcgaaaagag catttaatta aacgatgacc attatggatc
    15721 ttgctgctgc gtattttcgc caaaaagtat tgatcacggg gggagcaggt tttattggaa
    15781 gtaacctggc gcataaactg gcaaccctcg gtgcagatgt caccattgtg gatagccttg
    15841 accctgattc tggcgccaat ctctttaact tgaacggcat tgaaaagaca gtccgcttga
    15901 ttcaggcgga tatcaatgac gagaggcaga tggctcaagc agttagagga cagaaattca
    15961 tgttcaacct tgcggggcag accagccacc tgggaagcat gcaggaccca ttcaaggacc
    16021 tggacgtcaa ctccgtgggg cagttgcggc ttttggaagt ttgcaggcag aataaccctg
    16081 aaatcagtat tgtgtttgcg gggactcgtc aggtgtatgg gcgcccaaaa tacctgcctg
    16141 tggatgaaaa ccatttgttg acccctcttg ataacaatgg cgtcagcaaa cgggcgggag
    16201 agatgtatca cattgtctat aaccgtgtgc atggaatggc aacctgtgtt ttgaggatga
    16261 ccaatgtgta tggcccacgc atgcgcatca aagatgatct cctgaccttt attggctggt
    16321 ggtttcacca acttctgatg ggagaaaata ttcagatatt cggcgatggc aggcaggtca
    16381 gggatatcaa ttatgtggat gatgtgattg atgccatgtt gttgtgcgcg gcgcatcctt
    16441 ctgcaaacgg caaaatatac aatctgggcg gggcaccgat caatcttttg gatctggctc
    16501 gtttgatgat tcaggtaaat ggcaaaggtg gatacacact tgtgcctttt cctgagaatc
    16561 gcaaacgcat tgatattggc gattattacg gcgattacag cctcattcaa aaagatgtcg
    16621 gctggcggcc gcaggtggaa cttgaaactg gcatttcaaa tttgctggcc ttttaccaca
    16681 tgtataaaga acattatttg tgagtgaaat ggaagagatt cctgtcattg acctgaaaaa
    16741 acaatatcag tccattcgcc ccgaattgga tgctgttttt gcccgtgtga tggaaaaagg
    16801 cacttttatt ttgggttcgg aggtttctgc ttttgaaagg gaattcgccg aatattgcgg
    16861 cgcggctcat gcaattgggg ttgcctctgg tacagatgcc ctgcaattgg ctttgatggc
    16921 ttgtgagatc ggcgaaaatg atgaggtggc tgtcccggct cataccgctg tggcaactgt
    16981 cagcgcggtg gaagcgggcg gagcccatcc gcttttggtg gatattgatt ccacaaatta
    17041 cacgcttgac ccaaatctgc tccatcgaat tttgatgccg cacactcgtg cggtcatccc
    17101 tgtgcatttg tatggctgcc cggtggatat ggagcctgtg ctccaatttg cgcgcgagaa
    17161 tcatttgttt gtgatagagg attgctcgca ggcccacggc gcttcgtacc gtgggcgcaa
    17221 agttggtacg tggggtgata tcgccgcttt tagtttttac ccgactaaaa acctcggcgc
    17281 gtttggtgat gggggaatgg tggtgacgaa taacgatgcg ttggcagaga aggtgcgttt
    17341 gctgcgtcag tatggctggg gtgaacatta catcagttcg attcggggaa tcaacagccg
    17401 ccttgatgaa ttgcaggcgg ggattttgcg cgtcaaactg cgtcatttgg atgggtggaa
    17461 tgcacgccgc aggcagcttg ctgagttgta tcaccatcta ctttcaggga cagatctggt
    17521 tttgcctgcg caaaatgcgg gccatgttta tcatcaattt gtgattcgcc atgcacaacg
    17581 ggattcgttg cgggaatatt tgaaggaaaa tggtatttcc acactgatac attatcccgt
    17641 gcctgtccat tttcaacctg cttatgctga tctgggatat acagcgggga gtctaccgtt
    17701 cacggaacgg gcctcccgtg aagttttgtc tctgcctctt tacccggaat tgaccgaaga
    17761 aaaggtggtg aggatttgtc agaccatatt ggattttttc acgcactcaa tgtgacattt
    17821 ttcatgcctg catgacagaa cttttggctc gattggggta agtatagata agctttgttt
    17881 gtggctctcc aaagttgtga caaaaggaac tattctcttt aggcaagtta tgcgataatt
    17941 tacattaact gtcgtatttg aaactcttcg actttcaaaa tttcactgga ggcactatgg
    18001 caaaaatcac gcgcgaagat gcacttgaat atcaccgttt aaagggcaag cccggcaaag
    18061 tggcgatcgt tccaaccaag ccgatggata cacagcgaga cctgagtttg gcatattctc
    18121 ccggcgttgc cgagccagtt ttagagattg aaaagaatcc cgacgacgct tatgagtaca
    18181 cttccaaggg taatttggtg gctgtgatct ccaacggaac agccattctc ggcctgggtg
    18241 accgcggcgc attggctggc aaacctgtaa tggaaggcaa gggcgtctta tttaagaaat
    18301 ttgcggatat tgacgttttc gacatcgaat tgaattcaca cgacccggat gagatcatca
    18361 aagtagtagc ggcgatctca ccgacctttg gcgggatcaa tctcgaggat attaaagccc
    18421 ccgaatgttt ttacatcgaa gaagaactga agaagatgct gaacatcccc gtcttccacg
    18481 atgaccagca cggcacggcg atcatttcct ccgctggttt ggccaatgcg ttggaaattg
    18541 tcggcaagcg gcatgacgag atccgactcg tcatctccgg ggcgggtgct tcggcgatct
    18601 cctgtgccga attggcgatc agctggggcg tgaagcgtga aaatatcatg ctttgcgaca
    18661 gcaaaggtgt ggtgtacaaa ggccgcaaag agggcatgaa taaatacaag gagcgtttcc
    18721 tggtggaaac cgatgcccgc acactgactg acgccctgcg tggggcagat gtattctacg
    18781 gcttgtctgt tgcgaatgtg atgactcccg aaatggtcaa gttgatggca aaggatccga
    18841 tcatctttgc catggcgaac cctgacccgg agatcaagcc tgagctggcg agagaagcgc
    18901 gcaaagacgt catcatcgca actggtcgtt cagattatgc caatcaggtc aacaacgtgc
    18961 ttggcttccc cttcatcttc cgcggcgcgt tggatgtacg tgccaaacaa attaatgaag
    19021 aaatgaagtt tgcggcttcc aaggctttgg ctgcccttgc taaagaggat gtgcctgatt
    19081 cagtcatccg tgcgtatggc ggcgagagca ttaaatttgg gcgcgagtac attatcccca
    19141 agccgttgga tccgcgtgtt ttgttgtggg aagcgcctgc cgttgctgaa atggcaatga
    19201 agaccggcat ggcccgcaag accattgaca ttgaagaata caaggaacag cttgccttcc
    19261 gtcagggcaa gggtgaacgg atccgctact tcttccaaaa caaggcccgc acctcgggcg
    19321 gcaagaagcg tgtggccttt gctgaaggtg aagagcagaa ggtcattcgt gcggcctatc
    19381 agatcaagga agaagggatc gccactcctg tcttgatcgg gaacgcgggt gtcattgatg
    19441 aacaactcaa ggtccttggt ctcgactata agcccgtgat cgtggatccc aacaaattcg
    19501 aaaaattgga agcctacgcc aagtcctatt acgaaatgcg ccagcgcaag ggtgtgatgt
    19561 tgagcgatgc acataacaaa gtgcgcgatc ccaatattct tggtccactg atggtgaaaa
    19621 tgggcgatgc agatgctttt gtctcaggtt tgacctttga ttatcctgaa gtgatccgtc
    19681 ctgcattgca gattcaccat actgctcccg gcgcaacacg tgcggcgggc gtgtatatca
    19741 tgatcgtgga tgataaggtc ttcctgttta cagatgccac cgtcaatatt gacccaaccg
    19801 ctgaggatct tgcagagatc gcctctcttg ctgctgattt tgcagtgaaa cttgagatcg
    19861 atccgcatgt tgccttcctc tccttctcga acttcggctc caccccgcat ccgctttcag
    19921 acaaggtccg caaagctgtg gaattgacca agaaacgccg ccccgatctg cgtgtggatg
    19981 gtgaaatgca ggccgatacc gcggttgtgc ctgagatcag cgaagaacgt tacccattca
    20041 gcgctgtgaa ggatgcaaat gtactggtct tcccctcgct cgaatcggcc aatattgcct
    20101 acaaactgct ttcacgcctt ggtaatgcca aagccattgg cccgatcctg ttgggcatgg
    20161 gcgcacccat ccacgtattg caaactggcg atgatgtgaa tgccattgtc caaatggctg
    20221 cggttgccgt catggatgtg atgggacgtg aggcgaatgg ttaaagaaag tggttttacc
    20281 tggccatatt gaaaagtgaa agttgactac aaaaacactc tcaggatatt ttttcctgag
    20341 agtgtttttg taggcgattg ggaatagatg gaacattatc tgtttcttga attaagatca
    20401 aaagcctcca gcattttttc aacttctccc attgtgtaaa caaaatgagt gacagttctt
    20461 cgaaaaaaag ctttttctac aatgtcaaac gcgacctcaa gcatataatt tttgctttga
    20521 ttgacaacaa aacaggcacg taaattagct ggcggcagcg gaagtttccc ccacaaatct
    20581 ttaacagcgc caatggtgga ttgttcagga ggaaatttag actggtcatg aatgatgatc
    20641 gccttatggt caattgattt gaaataatgc tctgcctcct ttagcgcatt tactacgtca
    20701 ctccactgcc agggctttaa atactttacg cttaacactg tctttgttcg tgggtcttgc
    20761 cactcaatta cgaaaatatc attagagttc attgcaattt ttcctgtagt ctacctggtt
    20821 caggtaaaaa atctgtgttt ttctgtgtct caaaaaaaca aactgacggg tgactggagt
    20881 tttttgccac cccaattaaa tgtgccagac actttggtca tctaatattt tttcaatgag
    20941 ttttggaaaa gcgtacctgt ccaatggttt tgaaatggct ccatcaaaac cgctttcccg
    21001 taatttttcc acttcctcgt tcattacact tgcagtcaat gccactactt tacagtaacg
    21061 caatcgatta tcagatctga tcattttgag aagttcgaag ccatcataag gctgtatgtg
    21121 gatatcgagc aaaaagatat caggaatact gccaatctcc ctgaccttct ctagaaaatc
    21181 acttgtgttt tcaatgacga tcaagttgtc aacgatgcca agtacatggt tcataatagc
    21241 tttcataaca acgatgcttg gggcgtcgtc ttcaacatat acaatgatgg gtccgttttt
    21301 catgattacc tttgcgataa tattgggtat gtggggattt ggggataagg gagggagaaa
    21361 tagaatacag aaccttcatt aggctggctt tcaacccata gcttacctcc atgcgcttca
    21421 accaggtttt tcgaaattgg aagcccaagt cctgttcctt caactctctt acgaccatct
    21481 tctgtttggg tgaagggttc gaagatgatc gtttgtgcgg taatgggaat gccgggcccc
    21541 gtgtctgcta cggcaaaaag cagatagtcg gctctttttt tgagaataag agtgatgctc
    21601 cctttatctg taaatttagc tgcatttgac agtagattaa gcaacacttg acgaatgcga
    21661 cggcggtctg cttcgatgag tggaatatcg ctgtcaatat cctgaatgac ttcaacagac
    21721 tctttgatta atggggtgga cattttgagg gcagtatcga tctcttcgta aatgttgacg
    21781 tcttgttcaa ggttgagcgt gagttgtcct gcttgaatct tgctgatatc cagaacgtca
    21841 ttgatcaagt gcagaagatg gtgggagctt cgcagggatt gattgagaaa agcctgttgt
    21901 tcagtgttga ttgggccaac catttcagac gcaaccaggt cgatcaggtt gatgatggcg
    21961 ttcatggggg tgcgcaattc atgactcatg ctggcaagga attggctttt catgatatcg
    22021 gtccgtttga gttcttccgt gactttttcc tgatttcgat aaagttgggc attgcgtacg
    22081 gcaattgcga tctgttcggc aagtgtggag aatatccgaa tatcgttatc cttgaagcgg
    22141 ccgatggttt cagcttgtaa atccagtacg ccaacaagat catcaccaac aatcattgga
    22201 aatgcagcat gtgattttat ggatggaagg cgtgatgtgg gcacaaagga ggattgatct
    22261 atgttgttta aaacttctgt tttgcgagtg cgtgccacct ctgcggtaag gcttggccga
    22321 gtattgatgt gaaatgactt tggttccgtt ttcactccat tttcttttac atcgtcagct
    22381 tcgagctgca gttcgtccgt ttccggctgg tataagaata tcgaagtgga atacagatcg
    22441 aaaccctccc gtgtctgctt gaccagattt ggcagtagtt tctccagttt caattcctgt
    22501 gtgatctggc gcgacacatc agttgccatc tgcagatcac gagtacgttc ggcgacatgc
    22561 tgttcgagag attcgattaa atcgctcaac tgagcgctca tggagttgaa ggtttgtgag
    22621 agcgtggaaa attctttcaa gccgtggata tcgagatttt tttcgaaatc gccctgcccc
    22681 agacgttcgg ccgccagggt gagttgattt aatgggcgta aggtttggcg ggccagaatg
    22741 ataaatacac caatgaccga cagaagggat agactgcctg ctgtcagttg aattagcaat
    22801 gtggttctgt atgttgagtc gagttcttga ttcgccaata taaggccatt tctgctgacc
    22861 tctccaatgg ttttcgtgat caaatttatg tcaatggtga tatctctgaa aatggtagtg
    22921 ttcttaataa tatcctggtc tagggcaacg atctttgtga atgtatcgta gtgttcgagt
    22981 aggtttgtgg aaagttgttc ggcgtttagc gtctccaaac cgccttcttc agtggcttgg
    23041 gttaaatcca ccagttcaat ggtgttgata cgtacataat ctatatactc ctgcctgtaa
    23101 gtgctgaagt atccctgctc gttcttttgg atctgaatca atgtgtcgaa aaagaattga
    23161 ttgtcgaggc cgcgtgtctc aaattgcatt tcctcgattc cactggcaag ggatttttcc
    23221 agcccattgg ccccaatgcg tgtctgaatt tttcccaagg ttgcattgaa tgagttttca
    23281 taatcaccca atagagcaga taattggaca gtggaattgg cgatttccct gaagttttta
    23341 tttttagaat taattgaaag ggtttgcaat tcattgaggt gggtgcgagc tgcatcaaga
    23401 tggaactgaa tttgctcgcc atattctttg cgggcattat caaagccaag aagccgccag
    23461 tttgcaagaa aagcagattc gtcctgacgg gccagcaaaa attccgtctg gatttccagg
    23521 ctgagttccc tgatttgact gctttcctgc gaagtggctt taagtgcaga ttgaaaactg
    23581 ttgaaattta tcaggctaat gcccatacca agcaggttga atcccaatat tgccataaag
    23641 cccagaagca ataactcacg aactgataac ttatttaatt tcatagacct ggcctttgcc
    23701 aaaatttaaa aggcaacatg gattatttaa gcgcatcatg agcggcaagg atcatttcaa
    23761 atgctgcgtc gtagatattg ttggcttcct gcccaacgtc tacaacatag aacaaacgat
    23821 aacggatgtt ggtgggtgga taaatgatgg ggtttgtcag gtcgctttgt tcaataaatg
    23881 gcaatgcagc ctggttgggg gatgcgtatc gaatatagtt tgaaagttga gctccgatat
    23941 gtgggtccat catatagttg atcagctttt cggtttgttc tttgtgcgga gaagcaacag
    24001 agatgcacat attatccgtc cataagacag ccccttcttt ggggataaca tacttgatgt
    24061 cggggttttc tgctgcaatt tgcataacgt ctccgctgta ttcaaccgcg acatctacaa
    24121 tgccattgac aagcaaatcc tgcccgtcat caccacgcag tgtatagaat tgatcaatat
    24181 gttcgatcaa ataatcgcgg gcctgatata tctcactttc attggtggtg ttcgggctaa
    24241 agcctaaata taaaagcacc ccacccacag ctgtacggtt gctttccaca aatgaaacgc
    24301 ggcctttaaa ttgaggatca aataaatcgc cccagctgtc gaggtctctc cctgtcgctt
    24361 ttcgattata ggcaattgcc gtagttcccc attgatatgg aacacaatag cggttgtcag
    24421 ggtcatattg tgggcttacg aaaagaggat caaggttctt catattcggt atgttggctt
    24481 tgtttaaagg agcaagcagg ccatcccatt gtaagataga gactgcataa tctgaatcaa
    24541 taaacaagtc aaatgcttcg gggttttctt taagcctttt tcttaaatcg cggcttgagg
    24601 tatatgttcc ataattgacc ttgatgttgt attccttttc gaagttggaa agaatattgg
    24661 ggtccatata gttgtcccaa ttgtagaagt ttatttcttc cggggaaact tttgcttcag
    24721 gcgtttgttg ttgtgaccag ccttgacatg cagaaagtag tgccatcaag gtgagtatgc
    24781 taagacagag aactggttta agaatctttg tgctcatatt tggcttttac tctcttccat
    24841 aagatagggc aatgtgaaat aaaaagcggt cccttcacca agttgacttt ccacccacag
    24901 cctgcctcca tgtgcttcga caagcttttt tgaaataggc agtcccaaac ctgtnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatttc ttcatagatg ttaacatcct
    25081 gctcaatgta gagggtcagt tgtccagcct gaattttgct gatgtccagg acgtcgttga
    25141 tgagatgcaa aagatgacga gaactttgca tggattgatt gaggaatgtc ttttgttcct
    25201 cattgatggc acctatcatt tcagaagcaa ccatgtcaac aaggttaata attgcgttca
    25261 tgggagtgcg tagctcatga ctcatgctgg ccaggaactg gcttttcatc aggtcagtac
    25321 gtttgagttc ttcagcgact ttttcctgat ttctataaag ttgggcgttg cgaatggcaa
    25381 ttgcaatttg ttctgccaat gtggagaaaa tctgaatatc gctttcggca aagcgatttg
    25441 gatattctga ttgaaggtcc aatactccta caagttcctc tccaaccacc atggggaaga
    25501 cagcttctgc tctggtgttt ggcaggagtg ggttgaaaaa atgcgaatcc gatctggatg
    25561 tgtcgtttac gatttcagct ttttgttctc gtgcagattt ggaaaccagg cttggttgtg
    25621 cagagatatg aaaggctttt gcctgacctt tcatatgttc cccggatgca ccagaacccg
    25681 cctcaaacaa taattcctgg gtttggggat tgaaaagaaa cagagaaaca gcatacagat
    25741 taaaggctac ccttgttttt tccacaagat tagggagcag gttttctaga tttaattctc
    25801 gggtgatatc ccgcgaaact tgagaagcta cgttcaaatc tcgcgtacga gaagcgacta
    25861 tgctttcaag tccattgaga gtgttataga gttgttcaac catttgattg aatgcaatcc
    25921 ccagttgacc aacctcatcg ttcatttcaa tttttgtacg cctggaaaaa tccccctgct
    25981 tgatcatttc tgcgttttca gttaattgta tgaggggggt cgtaagatat cttaacaccc
    26041 aaacagttgt cagccagatc accccttcta tggtcaggag aatcatgtgt aattcttctg
    26101 gaatattggc aataaacgcg aaagcagtaa aaccaattaa agtaagttgg atctggacta
    26161 ccagaatttt ccctgcaagt tggatttgtt tgaaatttag aaagaagggt attgaatagg
    26221 ctatatctga tgcaataacc gtaccgccaa tgatccagaa accccatttt ggaatggcta
    26281 ccgaaaaatc caaaaaaatg gcggtaaaac acagtgaaag atagattccc agatgaataa
    26341 ggatattcct gaaagggagt ctttctggat acaaagtaat aaataaaata atgtgataaa
    26401 ttgcagctgt taacatgagc agcggcattt cggagagaaa aaagcaccct agaccagggg
    26461 caaaccagta gaatgtggtt ataacccgaa gtgcgatgta gtggttgttg tttgcagctt
    26521 gtctgtatga aagccagcct ccaaccccat aaaccatccc tgccgtgaaa aagcaaagta
    26581 gttgccacga gggtgattga ctataaatat aaataggtaa aatggttaca aaaggtgtaa
    26641 aacccaccat tagtaatgaa acccatttta gatactgata cccaattgaa ttggcaggta
    26701 atgtaggtaa agaagaagca ttcatacttg aattttgctt ttagtgaaag ttgtagaagg
    26761 caattattga ctgctgatcc atttcttttg gcctgcgatg atatctgcaa tatgttgtgc
    26821 aaacccatga acgcggatgg gcttgctgat gaatcccgaa aatcccatgg cttttgcctt
    26881 cggaatctct gtttcgggat ccaacgaagt gatggctata acaggaatat gtgctgtttt
    26941 ggggttttgc ttaatttctt tgaaagtatc gtatccattg acacctcttt ttaacatgat
    27001 atccaataag atgatatcaa ttggaagatg ctgctcaata tgcagagata ttccaatgct
    27061 gttgtagtta tagaagactg atgccccgga aaccgacaaa ggcctggaaa tgacactcag
    27121 gtttaataca tcgtcttcaa ctacaaatat tcttgtgttg agtagtggca ggttggtatt
    27181 ttccatcgcc gcgctcctta aaaccttgaa tttagtaaat gcgcgggcat tttatgtggt
    27241 aatcaggtag agcgctactc tctaaaaaga ggggaaaagg gtcagatcag cttgagcttt
    27301 acagcaatga taagtgcttg agagcgggta cgtaccccca gcttcccgca gatgttttcc
    27361 atgtggaatt tggcagtggt tgcactgatt tttaatcgag cggcagtttc tggcatattc
    27421 attccatctg ccatggcgcg tagaacatcc aattctcttt tcgtcaaacc cagagaactg
    27481 cctgtttgtt ccctgtctga aggggtggtt ttaaaggctt caggcgaaaa aaccattttc
    27541 ccccgatgga tcatattaat ggtttcggcc agatcgtctt taagtgtggt ttttaataca
    27601 tatcctgcag caccacttcg aagcatctca aacagcatgt cttgatcctg caaactggtg
    27661 aggataagaa tttttgagtt gggcattttg gcctgcaaag cttttgttgc ttcaaggcca
    27721 ttgataccag gcattagaac atccatcaaa accacatcag gatgcagttt ttcgcatacc
    27781 caaatagctt cctccccatt ttttgcatgt ccaaccagtt taatatctgt tgtctgattt
    27841 aagatggtcg taacgattcg atggatgtaa tcatgatcat ccactaaaac gacgcgaatt
    27901 gtgtttgggc tcatggtgaa attataaatg aagtctctaa aaattctcct tacagaataa
    27961 taatcctaac gcttgaacca gcgcccgaga gtaataagca aagaataaat agaaaaaacc
    28021 ttaaccccag cgtccttgtg gtcaaaagcg ggacatactc agttaccaaa agccgcaatc
    28081 tgggttactt attgtgtgtc tcacgcccat gatcacttca ctcactgcga gtggaaaacc
    28141 ttgaaaatcg gctcttttaa tagctataat ctctcgtttt caaatttgtt tcgttcacaa
    28201 caaggagttc catgaccaac ctcgaatcca tcgcacgaaa aattaccagc attctttttg
    28261 cccagcaatc ccttgcttcg gcagggttca tcgccgccgc caccctgaat tccattgttg
    28321 gcaaggaact cagcggacat tctgactggg cgggcgtccc ctctgcaacc tatcttttgg
    28381 cgggtgcatt tgccgccttt atgtggggct atgtttacga catgatcggc cgccgcaacg
    28441 gcttgaccat cggtctcgga atcggcgctg ttggttcagg gattgctttt tatgccatcg
    28501 cgatccattc atttgctatt tttctcggcg gcatggtgct gatgggcatt gcgaatgccg
    28561 ctgttcagct tggtcgtttt gccgctgcag aagtgaatcc tcccgagcat cgtggacgtg
    28621 ccatctcgaa cgtggtgatt ggcggaacgg ttggttcagt gatcgggccg tttgtcgctg
    28681 ggccagcagg agacctgatc ggttcatggg gtattgatga attggcgggc gcgtatctgg
    28741 tcagccttgc cttgttcgtc atcgctgcga tcgtcgtgtt catcggcctt cgccccgacc
    28801 cgcgtgagat cggaaaacaa gtcgccgaaa aataccccga taccatcgtc cgctcacgtg
    28861 tggtgcgcag catgatgcag atcttcagtc agcccgccgc cctcgtggca ctcaccagta
    28921 tggtgctcgg acaaatggtg atggtgttgg tcatggtcat cacttctctg cacatgcgcg
    28981 gacatcatca tgacctgacc gatatttcac ttgtcatttc gtcacacact tttggcatgt
    29041 atgccttttc catcatttca ggccgtcttt cagatcaatg ggggcgtgga cctgtcatca
    29101 tcactggttc gctcacactc gtcattgcct gtatcgctgc caccatttcg cctgatgtgt
    29161 tgccgcttgg tgtcgccctg tttttgcttg gcctcggctg gaatttctgc tttgtcggcg
    29221 gttccaccct acttgccgat caactttctc ctgatgagcg agcgcggaca caaggtttca
    29281 acgatctgct tgttggtctc gcttctgcca ttggaagtct cagcagtgga ttcatcttcg
    29341 ccgcacttgg gtataacccg atggcattga tcagcgctat ttttgcactc atccccctgt
    29401 tgctcgcttc tttctggacc ttgaaacggg cgcgaaaacc ccgggcaatt gcctagcctc
    29461 agcagttcgt cttaatcaaa aaagtagagc gaggtgttat ctcgctctac tttttttcta
    29521 cttctttcct gatttggact tactgttcat cccagttttt ggtccacttg gatttggttg
    29581 tgagttgcct ggcatctttg gtttaggtga ggcatttccc aaccccaccc gatttccaac
    29641 caaagtgaat gctgcattga ggcttttctc agcggttgct tcatccagac cggtgcgctt
    29701 cgaaatctct ttaaccatgc cgctattgtg taacaggctt gagtctacct ggcctgcgcc
    29761 catttgtccc agcatttcat caagatcaaa tgtattcgag tcgcggcccg aggtggggtg
    29821 gtgggcgagt agtttatgtg ccacgaaaga gacaatgacc attgcgatct ctggcgaaat
    29881 gttgaccttc ttggccagcg gttcaatgaa tggctgcagc attgccatga tcgggtcatt
    29941 ggcttgctgc ccgccgccca tgatcccttc aagggcaccc atcatcccgc ccaagccctg
    30001 agtttgcggc tggctcggcg tgttttgagc ttgttgtgcg ccaagcaaac taccgaccaa
    30061 acctgccaca tcacctgcat ccactccatc tgagaggttg atgccacctt gagattgccc
    30121 gccgccaagc aaactaccaa ccagactggt cattgggtcg gcttcttgag ccgaattgcc
    30181 ctgttggcgt gagttaataa ggaccttgag gatatcttcc attgacatgt tgttcttctc
    30241 ctttttataa caaaaagcgc agcgcgattt gcgctacgct tgcaaaaacg gctgagttat
    30301 tccgagtcgc cgcctgaggg tgacttgctt tcagtggacg cgctttcttt cggtgcgctg
    30361 tctttttcct tcttgctggt cttctttgta cttgaagtac tcgaagattc tcccgaaggc
    30421 gacttgtgat cggttgcata gaaccccgaa cctttaaaga tgatcttggt gggggtaatc
    30481 accttcttga gcgccttctt acggcattcc ggacaggttt tgagaggggt gtccgtgaag
    30541 gattgatgac gttcgaactg cacaccacaa gcttcacatc ggtaggtata aactggcatt
    30601 tgtttttctc cttgctgttc tttctcgaca acgccgcgga ttatagcccg attgataggg
    30661 tggggcaagc cttgaatgcc tcatgttata attttctaac aattaatggc cagaccttga
    30721 tggtcatggc cttttgcgga gggcgctgtg ttaaagatcg agatcgtcta ttgtgcggtt
    30781 tgacattaca ccaaccgggc cgtgagcctg gccgacgatt tgttgaagaa ctttgagcat
    30841 gtcattgaat cggtggcgtt atttccctcg gacggtggca ggttcgaggt cagtgtgaac
    30901 gggcagttga tctattcaaa attacaggcg aaacgtcatg cagaagcagg cgaggtcgtc
    30961 aatctcatct caaaaatcgt atcataaagc aggataataa tcccgctcaa cgaagaggat
    31021 aaagaatggc aaaaaaaggc agagtgtttt cgggggcgcg cccaacgggg cgccagcatc
    31081 ttggcaatta tctgggtgcg atcaagaatt atgtcgccat gcaggcggat tatgagtgtg
    31141 tgtactgtat tgtggatgtc cacgcgttga cgacggtgga gaccacacag gaattaaagc
    31201 agaacacctt tgagatggcg ttggattggc tggcagcggg cattcgcccc gaagactcga
    31261 ttctgttcgt gcaatcgcat gtgcctgagg tgatggaatt acacacctac ctttcgatgg
    31321 ttactccgct tggtaagttg accgacctgc cgaccttcaa agagaaggtt cgccagcagc
    31381 ctgagaatat caactatggg ttggtgggct acccggtgct gatgaccgcc gatattgttt
    31441 tgtacaaaac ggatgttgtg cccgtaggca ttgaccaggc tccgcacatt gaatttgcac
    31501 gcgagatcgt gcgctcattc aattaccgct acaagaccaa ggctttggtt gagccgcagg
    31561 tgaagcatac ggaagtgttg aaggtggtgg gcctggatgg caaggacaag atgggcaaga
    31621 gtttgaataa ccacatcgaa ctggcttcca cccctgaaga gacggtcaag cgtgttcgtg
    31681 aaatggtgac cgaccccgcc cgacagcgta agaccgatcc tggcaaccct gatgtgtgca
    31741 atgtgttcac catgcacaag cttttctccg cacaggagga agtggatatg atcaacacag
    31801 agtgccgtaa ggcaggcatt ggctgtgtgg attgcaaact gcgctttgcc ggcaacctca
    31861 acaaacatct tgagccattc cgcgccaaac gcgccgaact tgcttcgaat ccgaatcata
    31921 ttcaggagat tttgaacgac ggtgccaaac gtgcccgcac cattgcccaa cagacgatgg
    31981 ccgaagtacg cgaagcaatg cagttgccgt aatttactga acaagccata aagtgagaag
    32041 taaaaagtat aaaatgcttt tgcttctcac tttttatatc caatttaatc catgcgttta
    32101 ttaattcaac gagtttccca ggccagtgtc accgtagaag agcagaccat ttccaaaatt
    32161 ggaaagggtc tgctgatttt gttgggaatt ggccatggcg acggtgaaga gcaggcagcc
    32221 tttctggccg aaaaaacagc aaacctgcgt atctttgaag atgaccaggg caagaccaac
    32281 ctttcagtgc tggatgtgaa gggggaggcc atcgttgttt ctcaattcac cttgtatgct
    32341 gatgcccgaa aaggccgccg cccatctttt attgacgcgg cattgcccga tgttgctgct
    32401 ccgctggtgg agcgttttgt tgaactgttg cgcagccatg gtgtgccgac ccaaacgggc
    32461 aggtttggcg ctcacatgga tgttgaaatt cataacgacg gtcccgttac catctggttg
    32521 gaaaaatgac cattaaacct aatttttttc aaaaattcgt ccatcgcttt ttaatgatgc
    32581 gctgggtctc ggcttttttg gcagtcagtc tgcatcgttt ggatgcgatc gtgctgaagc
    32641 tttcaagagg ccgccacacg gttaccgagc tcgtcggcct gccgatcatc cagatcacca
    32701 ccattggggc aaagagcgga gagccgcgta ccatgccgct ggtaagtttg tttgacggtg
    32761 agaagatcgc gttgatcggc tcaaactttg gccgtcagca caaccctggc tggtattaca
    32821 acctgaaggc caacccgcaa tgcgtggtgc agacccatgg tcgtgagcaa aagttcattg
    32881 cccgtcaggc tgtaggcgaa gaacgcgaaa aatactggca gatggcggtt tcatattaca
    32941 aaggctacga gctttacaaa gtccgcgctg cacatcgcca aatccctgtg atggttttgg
    33001 aacccatgaa ataattgtca agattagaat ttcatgataa agttgttttg taggatgcaa
    33061 gtagcttttc gttgtggaag tatgaagatg atggcccaaa gcattgcctt tgagtcgttt
    33121 tgtttaaccg cccgatgaaa gacatactgc gtcaccttat tttttttatt ttgctaggag
    33181 gcaaaaaatg tctgatcgtg taatcggtac ggttaaatgg ttcaacggca ccaagggctt
    33241 tggtttcatc gagcgcgaag gcggccccga tgtctttgtt cacttctccg ctattcgcgg
    33301 cgaagggttc aagaacctgc aggaaggcca gaaggtggaa ttcaccatcg aaaaaggccc
    33361 caagggtttg caggctgctg acgtcgttgt cctgtaattc gtaaccaacc aaaaagagtc
    33421 tcgctgaaaa gcgggactct ttttttgttt gatgggggaa tgggcgcatt gcgctcgacg
    33481 agtagattga ttctaattaa caagcagaat cttcccgctt ggatttttct gattaaattt
    33541 gcggcgccgc acccgcttct ttcggtgacc ataaactggc atccatttcg accgccattg
    33601 cccccgctga cagcatatcg gcggcgtctt tttccgtcca cacaccgcct gcgccaatga
    33661 tgggaattcc gctcatggca gcggcgcgga ccgtatttag cgcttgtgga aatatggagg
    33721 gtccgtatac cctgcccgtg gtcaatttgt ttttgtcacg cgaagcgacc aatgccccgc
    33781 gtggacaggc aatactgatc gctacggcgc ctttttcgat cacttttgac cccagcgaaa
    33841 gcacctgttc catgggtaga gagaagatca atggtacttc acccaggcac atatcaagcg
    33901 taagcaggag aatatcattt gaaagcaggg gggcaaatcc cagctcaacg gccattacgt
    33961 tttcgatgcc ttcgagcatg cgcaccattt gagcggtctc ttcggggcgg tcagccatga
    34021 gatgggggat gatgggtagg tcggcgcggt tccaacgggc actgtgtttt ttgatcacgg
    34081 ctggtagccc ggggttgggc aggccggtgt gtagcaggaa tccgccgggg aattcgatca
    34141 aagctggttg gggagtgggc aggcgcgggc gcagagagat cggattcgtg atgaaggctc
    34201 cgaacctgtc aaatggaatc cctgcacggg ggtcgggtga gaatcccagt gatcccgccg
    34261 cattgatgat tggtttgcta aaatagatgt cacgtttcat nnnnnnnnnn nnnnnnnnnn
    34321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34381 nnnnnnnnnn nnnnnnnnnn ggagtattta tgcaaccctt gcaaggaatt cgtgtgctgg
    34441 atttaagccg tgtgttggct ggcccatatt gtacgatggt tctcggtgac ctgggtgcag
    34501 aagtgatcaa agtagagccg cctgaagggg atgagacacg cgcctggggg ccgccgtttg
    34561 cagaggggga gagtgcgtat tatttgtgtg tcaatcgtaa caagcgtgat attgtcatca
    34621 acttgaagac cgacgaaggc aaaaaggttt tgcgtgatct ggctttgcaa agcgatgtgc
    34681 tggtggaaaa tttccgaccg ggtacgctgg aaaaattcgg cttggatttt gcgactttga
    34741 gtcaatcgaa tccgaagttg atctattgtt cgatcactgg tttcgggcag accgggccaa
    34801 tgcgtgataa gcccgggtac gattttatga tccaggctct gggcgggttg atgagcatca
    34861 ccggggagcc tgagggcgag ccgatgaaga ctggggtggc ggtggtggac ctgtttgcgg
    34921 ggcagaacgc ggtcattgcc attttggctg ccttgcaagc gcggaccttg actgggcgag
    34981 gtcagcagtt ggatattgcc ctgttcgatt ctcaattggg ctggctggcg aatgtggcca
    35041 gtaattattt gatctcagga aaattgccaa agcggcatgg caatgcgcac ccgaatattg
    35101 tgccatatca aagttttcaa gccagtgatg gatggtttgc cattgcggtg ggaaatgacc
    35161 gtcaatttgg gcgattgtgt gaagtcattg gcagattgga tttggcaaat gatgaacggt
    35221 ttgcgaaaaa ttcggcgcgt gtgcaaaacc gcgaggagtt gatcggtttg ctgaaatcca
    35281 tttttttgat gcggcctgtg agtgaatggt tgtcggcttt ggatgaggcg gagattccct
    35341 gcgggccgat caacaccttt gagcaggtct tttccatgcc acaggtgcag gagcgggaga
    35401 tgctggtgaa aatggatcac ccaactattg gcgaattacc gctggttggc tcgccgttga
    35461 aattcagcga tacgcccgtg cagtatcgcc tgcctccgcc tttgatgggg gagcatacgg
    35521 aggaagtgtt gagggagtta cgagatagag ggacgaaagg agaataggcc atacactaac
    35581 gtgctggcaa aatttaaaaa tgaaatggct gatccctttt ttcttgattc tgcttggcgt
    35641 atccattttt tgctgcggat ttatgtatga cgccgtattt gctgggatac cctaccaaga
    35701 cccaaccccc gagttggttg ctaaatacaa ttttcattcc gaggttgctt ctgtgatccg
    35761 ttggggtggg gcagggactt gtgctttcgg cgttgtggtt gcgatcggtc ttgccctggc
    35821 aggaatcata cgaccgcgca ggcagaagaa tacctgaaat gccgaacttc atcatcaagt
    35881 cattagactt gatcagaaat aaaatctgga cgctgattca cacagaaacc gcagattttt
    35941 ctttttgtat cagcgttttt cagcgtccaa ataggttctt tgcttattac cgatgaagtc
    36001 tattggataa atccactcgg cccgattttg cacaattggt gaaagtggta atcattaatt
    36061 gatgtcggta acttggggta atctacgggt cgtttctttt tttgatgtga tacatcgtca
    36121 atagcggact ccactgcttg cggaggatgg tcaagccgcg tttggtgaga tgctcatcca
    36181 acaccttcag cccgaaatcg gtgtcgggat gtccccagcg gaaaaggaaa tcgaaggctt
    36241 tgatatccac cccgcgcggt tcttcgagga agagactgcc cccaggtttc agtacgcgga
    36301 cgatctcgtc aatggctttg cgccaatcgg ggatgtggtg aaggacgccg aaaatgatga
    36361 caacatcctt gctggtagtg gaaaattgac tcagatcggt ggcatcttgc aacaaaaatt
    36421 cgaattgcgg atatttcacc cgtgcaagtt cgatctgctc ctccatgaca tcgaggccga
    36481 tgtagctctt cgggttgagt tggttaagca ggtctgcgcc atagcctgaa ccgcagccga
    36541 tttcaagcac atccttgttt tgcagatcca atcccatgtg acggaagact ggcatttcaa
    36601 agaagcgttg ccccaacttg cggggaagtg attgcatggc acggaattcg ggggtggata
    36661 gtttcataaa aaatctcccc ttccttgatg aaagaaaggg gagaaacctt acgctgtttc
    36721 catgaaaccc atggcttgca gcatctcttc gttgctggca aacttgaatt cctcaagtcc
    36781 gcgttttctg gcctcttctg cttccacagc ttcgagccgt ttgatatctt ccttcaagat
    36841 gatgggtttg ccgagatttt tcattttttc agcgacaact tcggcgttgg cattggcggg
    36901 ttgtttggtc tgcaaatatt gccagacggc cttggcggcg tttgtcccat ctttgcgggc
    36961 ataaccgacc aaaccctcac tggctttacg agaccagccg ccgacgaaaa tcccttccac
    37021 tgcggattcg aaggtgagat tatctacagg gaaacgcggt gaattatttt tgatgaactc
    37081 gttccattct gtgggcaggc caaaggattc atccacttta tcgccgatgg caaagatgac
    37141 cgtatccaca tcgagggtgc gtttggcgcc tgtgcctttg gcgctggtct tgccgtctct
    37201 ttcggtgagg atgttatcct ccacttcaag tttggtcagc aagccgttct ccccatacat
    37261 ggcagtgggg gaggcgagga agtcaaagcg gaatttgccg tgggcggttt tggggtctgc
    37321 tttcgcgaga gaatccaata ttttttggcg accagtttca aggtcctgtt tgaccgcttc
    37381 aacggcaggg cggacccgtt ccatttcacg gttaaaatca tcaaggtcaa gatatgagat
    37441 caaatgcttc atctcttcct tggtgaaatt cacctcggct gggccgcgcc ttacaatggc
    37501 tgtcacctca tccacatttt gcacgtggag cagatgacgg gcaatatcca ccatcacatt
    37561 ccctgcacca atgatggcac agcgttttcc aaagtggaat tttttctggc tgaagggcgg
    37621 gagcttgttg tatgaaaata caacgtcttt ggcgtgatac acaccttcaa ggttttcacc
    37681 tggcaagccc agtgattttg tgccttgtgc ccctgcagtg acgagcacgg catcgaagcc
    37741 aagcgcgcgt aattcatcga gggttaagtc tccattggcg ccgatggtga cattgccata
    37801 gtaatcaata ttggcaaggt caagcacctg acggaattgc ttgcgcagcc cttctttcat
    37861 tgtatgtttg tctgggtaaa ttccatactc ggcgagaccg cctgccttca catcgcggtt
    37921 gaaaacagcc acatgcgcgc ccagtgtggc cagctcacgg gccccaaata aaccggcagg
    37981 ccccgcgcca attacggcta caaaaaattt attactcact tgcacgtacc tcctgaattt
    38041 gacaaaaaca gtcgtattat atttgaaaaa attcccatcc acaagcagtc tcatcctatg
    38101 ctaagattac agagtgctga gcaattggaa tcgtgctaaa ggagatcaca tggctgaaga
    38161 taacaaaaaa gaccccgcgc aaaaaatgcg atacctgctc aatggaggaa aaccctcccc
    38221 agtggcgcgc cttcccaaaa aggatggcgg tgtggcatcg ttccttccaa aattgaagaa
    38281 ggatgatgcc ccaacggaaa cccccgccgc tgtggaagtg gatgttgctc ccaacaagga
    38341 tcaacccgaa gcagcgcatc agactccgcc gaaacctgct ccatttcaag gggcattatc
    38401 gagcctgaag ggaatcaaac tcaacatcgg cccagctttc tggaccattg cgagtgtcat
    38461 ctcaatgacc gtcaatgtgg ttttgctgat cgttctactg gccctgcttc caaacctcac
    38521 caaattaaac ctcggcagcg cgttggatat tggtaattat ctgctgggcg gcctgtacag
    38581 caattttgaa aaaatggatc gcgcccacat caccacatcc attccagttg aaaccaccat
    38641 tcctgtgaag ttcgatttgc aactcaatca acagaccaat gtggtcttaa gtcaggatgt
    38701 gacgattacc aatgccctgg taacggtcaa cacaggcggt ttgaacatca cccgcgcatt
    38761 aaccaccatc gtcttgccgc aaggaacaaa cctgcccgtt gtgctaaact tggtcgttcc
    38821 agtggatacg actgtgcctg tggtattgaa tgtgcctgtg gatatcccgc tttcgcaaac
    38881 tcaattgcac gaaccctttg caggcttgca ggatgtggtc aggccgtttt attgcatgct
    38941 cagccccaat gcccttggca ttgatggaca gccagtttgt aaataacgcc aggtatcctg
    39001 gtggattagg ctgaacaaca gcgatcagaa tagattaaac ctatcggaga cagatgacaa
    39061 atcaaatcac gcaggtcaca ctgaagaacg gcatgaaggt catgttaaaa gagatccata
    39121 ccgcgcccat catctcttca tggatctggt atcgtgtagg ctccagagat gaacccacgg
    39181 ggaagacagg catctctcat tggaccgaac atatgcaatt caagggaacg aagaaattcc
    39241 caggcaatgt attggataaa gccatttcgc gcgaaggagg acgttggaat gcgtccactt
    39301 ctcgcgattc aacgcgctat ttcgaaacca tgcccgccgc taaaatcgat cttgccttgc
    39361 gcctggaatc tgaccgcatg aagaacagcc tgtttgatga aaaggaggtc gcctccgaac
    39421 gtacggtcat catgtcggag cgggaaggct tggagaatga accaactttc agattaagtg
    39481 aggccgtgca acagaccgcc tttcatgtgc atccctacca ccacgagatc atcggcgata
    39541 tggctgacct gcgcacgatc acccgtgatg acctgtacga acactatcgc aggtattacg
    39601 tcccgaacaa ttccattttg accattgcag gcgatttcca aacgaaagca atgttgaagc
    39661 gcattcagga attgtttgag tccattccga tgggcaaaac gcctccacgc ctaaatcgcc
    39721 ctgaacctga gccgaaaggg gaggtgcgcc tcactgttga agggccaggt gaaactacat
    39781 ttattcaggt ggcctatcgc ttcccaaatg cagcgcaccc cgattatttc ccattgcagg
    39841 tgcttgacag cctgcttggc ggtgccagcg gcctttccta taaaacatcc cgcctgtacc
    39901 gtgccttggt ggataaggaa ttcgcagtgg atgtatccag ctggccggaa gcgaccattg
    39961 atccgttttt gtatcgcatc attgtcacca accatcccaa acgaaaaaca gaccagacac
    40021 ttgccgccct tgatgccgag atacaaaaat tgcaggaaaa acgcgtatcg aaagatgaaa
    40081 ttacccgtgc tgtaaaacag gcacgcgccg tttttgcgta tgggagcgaa aatatcacca
    40141 atcaagcttt ctggatgggg tatgccgaga tcttttcatc ctatcggtgg tttaccagcc
    40201 atttgagcaa ccttgccaaa gtgactccgc aagatgtgca gcgtgtggcg cagaaatatt
    40261 tccagcctgc caatcgggtc atcggcatct acacacccaa tggacggagg agcaaatgac
    40321 caaacctgtt tctgtaaaaa agaaatcctc cctgcctggg cctgaggata ttcaccgcga
    40381 gacactacca aacggcatca cagtgttggc gcgctccaac ttcaacagtc catccatcag
    40441 catggctggg tacctgcccg cgggcgcgat ctttgagacc gacgataaac tcgggttggc
    40501 agattttgtc tctgcttcgt tgatgcgtgg cacacagacc aaaagtttcg atgcgatcta
    40561 taacgagctc gaatctgtcg gggctgggtt gggctttgat tctggagttc acaatgcaag
    40621 tttcggtggt cgttccttgg tggatgacct gccattaatg ttgaagttgt tgtctgagtc
    40681 gctgcgcaca cctgtttttc ctaagaacga aattgagaaa ttgcgagcac aaattctgac
    40741 cggccttgcc atccgcgaac aagatacatc tgatatggct gcaatggcct ttgaccgctt
    40801 gttattcgat ggacatcctt atagcaggcc gtcagatgga tttgtggaaa cgattcaaag
    40861 catcacccaa aaagacctgg tcaactttca tcgcgatcac tatggaccca gaggcatggt
    40921 cattgcaatc gtcggtgcga tcgacccgaa gaaggctgtt gcagcggtgg agcgtgttct
    40981 cggagggtgg caggttccag gtcaggttgt gccgccgctt cttcctccat acaagccgat
    41041 caagaaaacg gtcaggcatc accatacact tgcaaacaaa tcccagtctg atttggtgat
    41101 cggtatgatt ggtccaaagc gaaaggatcc ggagttttat tctgcttcgc tgggtaattc
    41161 ggttttgggt caatttggca tgatggggcg cattggcgaa tcggtgcgtg aaaaatcagg
    41221 gctggcctac catgcatcat caagcctgag tgcgggagtg gggcctggta cctgggaggt
    41281 gaatgccgga gtgagcccaa agaacttgaa gaaagcaatt gatttgatcg aagaagagtt
    41341 gcgtaaattt gtaaagagcg gtgtgacaaa acgggaactt gaggatagcc aggcaaacta
    41401 cattgggaga ttgccgcttt ctgttgagtc aaatggtggt gtagccaatg cgttgttaaa
    41461 tattgagcgt tacgacctcg gcttggatta ttatcagcgc tatgaagggc tggtgcgtgg
    41521 tgttacgcgc gatcaggtgt tggctacggc acaaaaatat attgacccga atcgcctggt
    41581 gatttcaaca gctgggccgt aattgatttc gtatatgaag cttgctactg gagttgattt
    41641 aattgaagtc agccgtgtcc gtgaggcgat tgagcgccac ggggagcgtt ttcttgcgcg
    41701 gatctttacg gaaattgagc aacgcgaatg tggcggaaaa tttccatcac ttgctgcgcg
    41761 ttttgccgtg aaggaagcaa cagccaaggc cttggggact ggcattggtg atgtcagctg
    41821 gctggatatc gaagtgcggg gcgatgagaa tcgtgctccg cacttgtatt tacatggggc
    41881 aggggaagag ttggcccaaa agctgggatt ggtgacctgg tctgttagca tcagtcatac
    41941 cgagacacaa gccattgctt ttgttgttgc cattgggtaa tgaaaacatc ccgcgaccag
    42001 tattcgcggg atgttcgtaa ggacggttga gatgtgggaa tggcgaagga cttggagtcc
    42061 accacatctc aattcatttt tggtcctcaa cgccgatcca aaggtgccca ggcatgacgg
    42121 gtttgtaggg gcggataatt aatttcccaa gttcaacttg tctttgcccg ttttccatca
    42181 tgctagggtg gacaaggcat aagtcgggaa tgtggccgaa tttcttgcga tagaaatcca
    42241 tggctttttg aattttaata ctcaaagtgg ttcgtggatc attgtcaaac cataacattc
    42301 cagtatgcat gatgaagtcc tttctcaaat tgcgtttgtg cgtcaatcca gtgcgggagc
    42361 taaaaacctg agccagttct gtccctcacg cattgcgaat tgtgagcccg ttgccagtgt
    42421 gccaaggatg tcgttggatg cgttcgcaac caaatgcgcg tcttgcaagt tctggatatt
    42481 gccaaacagg cgtgtgcgaa agaatccaag ccatgtttca atgtgatgtg cgcggcttgc
    42541 gttgagagtg acaattggcc agactttacg ggaggttccc cgtaacaaaa gccagcgcaa
    42601 acattgttca gtctgctgat cgagtttggt tagaaactca agatcgtcaa tcaaaagcaa
    42661 aaaagtgcgt tcatcgtttt tgttttggtg tgcccattca acaagagact ggataagttc
    42721 ttgtgcgcca ttgtcttgcg tggtataaac tcccatattg ctctcacgat cttgaaagtt
    42781 attccactct tctgcatgtg atgtgacaac acagtattga atatctgaag gagagtggag
    42841 taattcggca gcttgagcga tggtttgcaa gagtccggtc tttccacaag ctttgtcacc
    42901 aacaatcaag attggccctg gaatcggatc gtcaagatta agtaaaactg gcaatccatc
    42961 ttctgcaatg cctaaaaaaa gagcttcttc aggcaaagga gtaatgtctg caaggacact
    43021 gtttaatgtt ggcaggataa tttcaggcat gcgagaagtg tgaccagact gttcttcttt
    43081 tgccagttca gtcaatgcat tgatcattaa ggtgaagtga ttggaattgt ccattattag
    43141 aacgcctgtt ctaataatac aaccaaagag tttgaatgtc aaggcgcaaa gttgctgtcc
    43201 aatttggaaa taatgaaagt gcaataatct tgacggtatg cgttgttttg gtataatccg
    43261 cctgcttaat cacttgccga cgtagctcaa tcggcagagc acccgccttg taagcgggcg
    43321 gttacgagtt caattctcgt cgtcggctca atggttgctg aaaaaggcgt cggtgatcag
    43381 taacaattta tcaagggcag ttacccaagt ggccaaaggg gactgactgt aaatcagttg
    43441 tcagaagact tcggaggttc gaatccttca ctgcccacta ccggtgtttc attaccggtc
    43501 agcacgttaa gccctcatag ctcagtcggc agagcacacc cttggtaagg gtgaggtcac
    43561 gggttcgaat ctcgttgagg gctcagttgc tgaaaagcat tcatgtaaat ataaaaagga
    43621 gaccgagaga tggcgaagga aaaatttgat cgcagcaaac cgcatctaaa tgtagggacc
    43681 atgg
//
LOCUS       DAOQLC010000018        42881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_250_length_42881_cov_21.681572, whole genome shotgun sequence.
ACCESSION   DAOQLC010000018 DAOQLC010000000
VERSION     DAOQLC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 42881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42881
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_42881_cov_21.681572"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1811)
                     /locus_tag="PL000_04990"
     CDS             complement(201..1811)
                     /locus_tag="PL000_04990"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNC88280.1"
                     /translation="MTTKYLFFTGGVVSSVGKGVTAAAMGLLLKERGFNVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDNRLTRVSNFTTGQVYAETIAK
                     ERRGDFLGGTIQVIPHITNEIKRRIGLAAKETGAEIMILEVGGTVGDIESQPFLEALR
                     QLRNEVGRENCLFVHVTWLPTIGATGEIKTKPTQHSVAALRSIGISPNIIIARADQDV
                     DKGLCEKIALFCDVEKDAVIPMKTADVLYEVPLLLEKLGVGELFLEKLGLKAKRKPNM
                     RPWKKLVEDVRKPKPTVKVALVGKYVELQDAYMSVREALKHAALANDVEVEINWVHSA
                     DLEKDKGWDIVKKSDAVLVPGGFGSRGIEGKVMAARFARENKVPYLGLCLGMQVMCID
                     FARHVLNLEDSNSSEFDRGTEHPIIDLMIDQRSITDMGGTMRLGLYPCLLQKGTKAAT
                     AYGVAKVDERHRHRFEFNNSYRADFEKHGMVFSGLSPDSRLVEIVEISDHPYMVASQF
                     HPEFLSRPMRPHPLFVGLLKAAKERAKK"
     gene            complement(1937..2311)
                     /locus_tag="PL000_04995"
     CDS             complement(1937..2311)
                     /locus_tag="PL000_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNC88281.1"
                     /translation="MAKSRSDQMVERLRNMQAAAPDIEASAVVSVDGLIMASALQQGV
                     EEDRVSAMSAAMLSLGERISSELGRGGLDQVYIKGDKGAIVLTAIGDEAVLTAMSRPD
                     GKLGMIFLEMRRAAEDLVKLVG"
     gene            2510..3115
                     /locus_tag="PL000_05000"
     CDS             2510..3115
                     /locus_tag="PL000_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88282.1"
                     /translation="MLKLPVIFALFFWMQSTTAAITSPQPGDTLRGQVEILGTMDVPN
                     FASAELAFSYDAPSPAESWFTIQTFPQPVQNPTLAVWDTTTVTDGDYTLRLRVFLTDG
                     SSQDVVVSGLKIRNDEPLTTATPTSTAQADIQLFETAAPPTLTPRPQPTLQTFPSPTP
                     LPPNPASVTSSSIYVNFGRGALIVLVLFIFVGIIFRFRSRA"
     gene            3112..>3351
                     /locus_tag="PL000_05005"
     CDS             3112..>3351
                     /locus_tag="PL000_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC88283.1"
                     /translation="MTRPRACAAIVQNKKILMVLHRSSSREYWTLPGGGIEPGETPEQ
                     TAVREVKEETGLDVTMGNLLFFEPSGEYCFSAELAN"
     assembly_gap    3352..3451
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3491..4075
                     /gene="pth"
                     /locus_tag="PL000_05010"
     CDS             3491..4075
                     /gene="pth"
                     /locus_tag="PL000_05010"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC88284.1"
                     /translation="MTDTTYLIIGLGNPGREYKDTRHNIGFMLVDQLAVRLDARGMKL
                     QSKAIVISAHHEERKIILAKPQTYMNLSGQSVQGLLHFYKIPIENLLIAHDDMDIPFG
                     TIRIRPGGGPGGQRGMASTIDHLGTKEFPRLRLGIGRPPGRMDPKDYVLQMFSKEDMK
                     LLPDVLSRGADAALEFVLKGLNSAMNKFNGEVGK"
     gene            4133..7525
                     /gene="mfd"
                     /locus_tag="PL000_05015"
     CDS             4133..7525
                     /gene="mfd"
                     /locus_tag="PL000_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNC88285.1"
                     /translation="MQPLLSNIRTLPAYQQLLVELNAGKTIAGLGLSRSARLPVLAAL
                     QADLNRPILLVTDRADHALSIFDELDFWVKSPRLLFAEPNPLFYEEAAWGVSTRRSRL
                     QTLTTLSTYHLPFSKKPETPPIIVASVRSLMTRTLPRRDYLKACKKLSVNQRIQPDGL
                     IREWARIGYQRVNTVLEPGQFSHRGGLLDIWPPTELNPVRLDFFGDEIETIRQFDPAT
                     QRTVEKLENILITPAREYLQPEGEPSENLSEFHIPLLHSQPASVLDYLPPQALVLMDD
                     ISLVETMANEIEEQAVKFRGECIAEGTLAAAFPVPYVPWSDLNDDIHHMPCLELGHST
                     ELLESESSVDLASNFSHDERFGGRLKPFIDYLASILERDEQVYIVSRQTPRLRELWAE
                     HYPNSEPENLSFLEASLSEGFTFQTSHLITDSEIFGWERPQPRIRQKQVAETPESIYA
                     DLQTGDYVVHVDHGIGRFNGLIQRQLDGHEREYLTVEYDRGDILYVPVHQADRLTRYV
                     GADGGAPSLDHLGGQAWQETKAKVKEAVQQVAEDLLDLYARRQVVEGYSFGADTQWQK
                     ELEDSFPYVETEDQKRAILDIKRDMERARPMDRLLCGDVGYGKTEVALRAAFKAVMSG
                     KQVAVLVPTTVLAQQHFETFSQRLAAFPVKVEMLSRFRTHREQTEILYKLALGEVDIV
                     IGTHRLISSDVEFKDLGLVVIDEEQRFGVTHKEHLKKLRTEVDVLTLTATPIPRTLYM
                     ALTGVRDISNLNTPPEERLPIVTHVGPYSPRLVRQSILRELERGGQVFFVHNRVNTID
                     AMKAHLNKLVPEARVDIGHGQMPEGQLAAVMHRFTTGEIDILLSTTIIESGLDIPNAN
                     TLIVDRADTFGLAQLYQLRGRVGRGAMRAYAYFFRHNKMSPTQEGQQRLEVIAENTQL
                     GAGYSIAMRDLEIRGAGELLGTRQHGYIQSVGFHLYTRMLADSVRRIRVLMKDGLNID
                     VPSALLPSAFSLPLSMPVNVDLPVAVGIPSDYISDQDLRLRLYRRIADLRDETELDAL
                     GSEFRDRFGHLPEMVQNLLYQMRVKLRAEKAGLSSVSWEAGQILLKYPAPAEGVEPKR
                     LPDLGEGVRGGKSAYWVTVAKEEAWTIRLLDVLDRLLKSPAS"
     gene            complement(7592..9634)
                     /locus_tag="PL000_05020"
     CDS             complement(7592..9634)
                     /locus_tag="PL000_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88286.1"
                     /translation="MKRIRSVLQNILSQEKYTPLAFLVVAFIGFGLLANRLGFYQDDW
                     PYIFYAFNKGIPSLTLELYYDSRPNAAWLYIVMFNLLGFKPIAWHLAAVIFRWLTGTL
                     LWYLLRRIWPSHNKQVTFATYLFIVHPFFLIQPYAVNSILYWAGFLFFSASLLIMARN
                     ATESKHRISLKLLAVFLEAVHLFTSEYFVGMVMVRPLILYWVLNSPELPTRQRIMTAI
                     KQWIPYLAALAAYIIWRMFLYVPPPIGDRNAPEVLFALFSKPLPTIAYLIRIALQDSV
                     IITFTSWYRTLVPELFAFSSIFSWFVILVSIFAFVAVFIHLKQITEKEERGSWFAQPL
                     TLGLFLLLLGMAPIWVIGQDIVTHKNQFAGTRFGIGSTLGAALILAVLIDRLIDGRKK
                     IVVVSACIALAISMHLTNEKDFAYSWEKQERFAQQLLWRAPKIEAGTALVTDEEILGY
                     MGSYSVSYSIITTYQPGDIQTPPHWYFPFYYTNPNVNDFLAGIPLEDSKLTMEFNGNS
                     DKMILFTFNPEMQRCLWVLRPEDTNLRLISTDMRQLSAGSDVELIQQGEKSPPEQIYG
                     KQNTDTWCYFFEKADLARQYKDWDSVVKLWKESQAIGEQADNGFEIIPFIEGYGHLED
                     WEQVKTLTKSAKRITAGLEPSLCGMLDRLALDTPASQERDDTLLKLKEDLNCSNYQ"
     gene            9775..11814
                     /gene="uvrB"
                     /locus_tag="PL000_05025"
     CDS             9775..11814
                     /gene="uvrB"
                     /locus_tag="PL000_05025"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNC88287.1"
                     /translation="MDFKLTAPFIPMGDQPEAIRGLVDGVRKGMKHQVLLGATGTGKT
                     FTIASVIQELQKPALIMAHNKTLAAQLYAEFKEFFPENAVSYFVSYYDYYQPEAYVPR
                     HDLFIEKETQINEEIERLRLAATTALVSRRDVIIVASVSCIYGLGNPEEFNKGSVNLA
                     VGQLVRRNALLRRLIESQYQRNDMELRTGTFRVRGDTLEIIPAYEEKRAYRITFFGDE
                     VERIMQFSPLTGEVLEEPREISIYPAKQYLTDAEKTKEAMADIESELEERLAQYKQAG
                     RILEAQRLEQRTRYDLEMLREVGYCSGIENYSRHFDRRAAGTHPWTMIDFLPSDYLLV
                     LDESHMTVPQIRGMYNGDRARKETLVEYGFRLPSAMDNRPLKFDEFEEVMGSTIYTSA
                     TPSAYEMDHAEQVVEQIIRPTGLIDPEVDVRPTRGQVDDLVGEIKQRVERRERILVTT
                     LTKRMAEDLTTYMKELGIKVQYLHSEVETLERVSILRDLRLGTFDVLVGINLLREGLD
                     LPEVSLVAVLDADKEGFLRSDTALIQTIGRAARNVNGRVIMYADRMTDSMKRAIDETN
                     RRRAKQMKYNEENGIVPISIHKEIHDLTEEMSAKAVGEMKGEYKVKGSQGMPRGELRK
                     IVDEVEKQMKEAAKNLEFERAAALRDELYELKTLLAEDESLKPWERIKLMTGEDD"
     gene            complement(11880..12308)
                     /locus_tag="PL000_05030"
     CDS             complement(11880..12308)
                     /locus_tag="PL000_05030"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNC88288.1"
                     /translation="MYSPRHLEWTNALSTGDASLDSQHRYLVDTLNKLGDVINEGHGG
                     EELARILGVLRFYAGWHFGKEEDCMETYHCPAAEKNKHAHAVFVEKFDKYHAEFTQSG
                     GSNEMALKIHEEISDWIVNHILIVDGELYPCIHHKPKPSR"
     gene            complement(12434..12691)
                     /locus_tag="PL000_05035"
     CDS             complement(12434..12691)
                     /locus_tag="PL000_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HNC88289.1"
                     /translation="MDELTYEKIGEANSRAEAEVIESFLEAEGIDVELVEESISRTAY
                     TLATARVDIFIPKDEVKRALKLLQPFDDIQPDDEEEGNDQA"
     gene            complement(12725..13882)
                     /locus_tag="PL000_05040"
     CDS             complement(12725..13882)
                     /locus_tag="PL000_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC88290.1"
                     /translation="MQKNTPLLLAGAGPFGLSLAAYLKHEKIEHIHVGASMDYWKNFM
                     PLKMFLRSACDWHLDPHNEATIVNYLKTLNLQPADVEPLSLDFYLTYIEWFQRQKGIS
                     PLPAWITKIDQAGNGFDVTFADGSSTLAEKLVMAVGLRYFKNIPEPLNSLFPSGRFHH
                     TAEYIDFSNLKGKRVLIVGGRQSAFEWAALIHEEGAQNIHMTYRHDTPAFTPSDWTWV
                     NPLVESMADRPEWFRNLAQEEKDAIIKRMWGEGRLKLEPWLAKRITQETIHLHPQTQV
                     ADCKELPAGELQITLNDGSTFPIDHVILATGYKMDIAQIPMLANGTLAAKIKTQNGFP
                     VLDESFQSSVPNLYFTGLPAAQDFGPFFGFTAAANTSARIIGRALSMKQNS"
     gene            14032..14523
                     /locus_tag="PL000_05045"
     CDS             14032..14523
                     /locus_tag="PL000_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88291.1"
                     /translation="MKKTILIVGLLVLALGVLGVGVVFAQGTNPPYAGHGPMRNGEGP
                     LHTFMVIEFAKKLDLNVNDINTRIAAGETMYDIALSAGVTAEEFPTVMTEVRTAALDA
                     AVKANVITQEQADWMKSRGFGQGGMGTGTCDGTGMQGAQGQGMGRGMMGQGRGWQNQQ
                     VAP"
     gene            complement(14591..15430)
                     /locus_tag="PL000_05050"
     CDS             complement(14591..15430)
                     /locus_tag="PL000_05050"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HNC88292.1"
                     /translation="MTQAAKAQSLLALHNNGKLLILPNVWNPIGARMLEVKGFPAVAT
                     ASAAIAESLGYNDGEQLKLESMLEMVRRIANSVEVPVTADFEAGYSETVAGLQQNISN
                     LLDTGAVGINFEDSWDDGARLRPIPEQVERIQAIRETAEKRGISLVINARTDSFFAEG
                     FASTEERIEDAVTRANAYLKAGANCIYPIGAGDEKTLKILRQRIHAPINVLASAKTAS
                     LKQLQEIGINRVSFGPYIFRSLLAKASAMYDEIKELGGYEVVTDGIFTGGDVQKYLLT
                     GKE"
     gene            complement(15440..16378)
                     /locus_tag="PL000_05055"
     CDS             complement(15440..16378)
                     /locus_tag="PL000_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC88293.1"
                     /translation="MSHSHNHSHFGDLATQTTKRLSLSLSLTAAFVLVEVAAGIFGNS
                     LALLTDAAHNFTDVIALGLSWYALKIALKPANAGKTFGYHRVGILVALVNSTTLILIA
                     LGIFYEAWQRFLSPPEVDSAILMGVGALAFFINAVTAWLVQKGSEHDLNLRSAFLHLM
                     GDVISTLGAVIAGVVIYFTKWNWLDPFVSVLIGAFIVWNAWGILRQTIHILLESTPEN
                     IDMPTMISDIRKVHGVRDVHDLHVWSISEGLRMLSAHVVTDEISLRDGTFIQNNINEM
                     LAHEYHIQHATLQLECEGCQSSDLFCSIAEHHHAHH"
     gene            complement(16422..17825)
                     /locus_tag="PL000_05060"
     CDS             complement(16422..17825)
                     /locus_tag="PL000_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNC88294.1"
                     /translation="MATELKPNDILSKAESLRNNLSSGFRDEIVKSLYSEAEAIAKRA
                     VKTADNKRYDLDQKIDRLVTSPITGLPIMLILLSLIIWLTVSGANVASDAIANVLFAF
                     GEIGRNFFNSLGFIPWWITGFIWDGVYLGLAWVVSVMLPPMAIFFPAFTFLEDLGYLP
                     RVAFNMDWMFKKAGAHGKQSLTMAMGFGCNAAGVIATRVIDSPRERLIAILTNNFVPC
                     NGRFPTLIMLGTVFVAAGFSGLTSSVIAAGSVVGVVLLGVVFTFLMSWILSRTVLKGE
                     ASAFTLELPPYRKPNFGRILYTSIIDRTIFVLWRAMQTAAPAGAIIWILANVHIGDTS
                     LARAIADWLNPFGLLLGLDGVILLAYIIAIPANEIVVPTMMMMYMGAGMMIDGPSSNT
                     AIYDLLVHGNGWTMLTAVNLMLFALLHNPCATTIMTIYKETKSLKWATLSVVMTLGTA
                     FLVTFLTASIARLFGWA"
     gene            complement(17827..18663)
                     /locus_tag="PL000_05065"
     CDS             complement(17827..18663)
                     /locus_tag="PL000_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNC88295.1"
                     /translation="MTNTKTVLPTEHCETCPAFTQLEQMGIKVGDFDRVIALAGNPNT
                     GKSTLFNALTGLKQHTGNWPGKTVTRAEGGFEFNRMRYKLVDLPGTYSLLSASQDEEV
                     ARDFILFGQPNCTIVVTDATALERNLNLVMQVMEITENVVVAVNLMDEARRKGLEVDT
                     RSLSRDLGVPAIPITARTGEGIHTLLGVVAEVIEGKVEIKPLRVNGTPEFQKAVNELV
                     PMIEQVAPGIPNARWLAIRLLDGDARVQKALTSGELADIVSRQQRMDVQFSRKMSVEG
                     NQ"
     gene            complement(18692..19696)
                     /locus_tag="PL000_05070"
     CDS             complement(18692..19696)
                     /locus_tag="PL000_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC88296.1"
                     /translation="MNPIILLILGAALSALIFLPRVGLIAVYKTYREAKEREHVEDAL
                     KHLLDREQQGRHASPVSLAGTLDLPLAKTTQLIENMETQELLETRGNDLHLTSKGERW
                     ALHVVRAHRLWERFLADEARMPLEKVHDEAQRREHTLTDAQLNELDAALGHPTRDPHG
                     DPIPTREGTLPKAEGMPITAWQADRPARIVHLEDEPAIAYQQILAAGLRLGQVIRIIE
                     KTQGRYLLSDGETEYKLAPAVASNVHVTALPEIESLKSEAVPLAELAHDQRGEIVMLD
                     DAVQGFTRRRFLDLGLTPGTLIYPELKNFFGDPRAYRIRGTLIALRKDQAAQIWVKPL
                     "
     gene            complement(19693..20298)
                     /locus_tag="PL000_05075"
     CDS             complement(19693..20298)
                     /locus_tag="PL000_05075"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNC88297.1"
                     /translation="MNETIFRGIAAIVLISGMAISSYHRIKADRHTGEKVSRKTDGSV
                     MMGIIKLFGLLLWLSPFVYLIAPQWMAWSKIGLPEWARWLGVGLGVASDGLIYWLFSS
                     IGDGISPASATRKEHKLSTSGPYRWVRHPLYTVGTSLFISFGLMADNWFIALLGVLAF
                     IVMAIRTPKEEANLIAKFGDEYREYMKTTGRFLPKLGGKPS"
     gene            complement(20325..20993)
                     /locus_tag="PL000_05080"
     CDS             complement(20325..20993)
                     /locus_tag="PL000_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC88298.1"
                     /translation="MEQNLTISIQDYLKHIYELTENGTSASTNALAAKLKVKPASVTG
                     MVQKLASAKPALVTYQKHQGVTLTDDGKKAALEVIRHHRLLEAWLVQTLGYTWDEVHE
                     EAEKLEHVISEDFERRIAAAMGHPLRDPHGELIPTADLEMPFEDSTPLSSLRPECIVA
                     IQCVKAADTDLLRYLENLGLVPGAEIKIKAYSPYDHNLTIQIEKESIVLGLNITSKIY
                     VKEI"
     gene            complement(20995..21681)
                     /locus_tag="PL000_05085"
     CDS             complement(20995..21681)
                     /locus_tag="PL000_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88299.1"
                     /translation="MNSIQVTGLLAIVGATLYAIGDVLLLANKVNIDDYPKLKPFQKL
                     LSDAEKFVSIPSEKMMWGALIGVFSTPLVLAGFWQVYQGLADINPILKTATALLFVCS
                     SVIGAFVHGSFFYAGEYVKALNEVSEDSQNIFVELFTRHRKVLVVTYAPLLVMVVIAS
                     VLFSVAVATQSTMFPAWMAAINPVTMTIAWLLSKRILPAFITDALEGAGFNVAYFIFF
                     TCTTITLWNA"
     gene            21841..22992
                     /locus_tag="PL000_05090"
     CDS             21841..22992
                     /locus_tag="PL000_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNC88300.1"
                     /translation="MKKATHQQTKQHNRDLVLRTIFLQESISRADIARLTNLTRTTVS
                     DVVAGLIAEGLVCEVGHGASIGGKSPILLSVIADARYVVGLNLAQDKFIGALVNLKGE
                     IKETIELDVHESDGEKGLQFVYQIMDQLFKKKLKPIIGIGIGTPGLVNTREGVVLNAV
                     NLDWQDLPLGALLEKKYKVPVSVLNDSQAAAIGEFVYGGDHRPDENLIVVNARYGIGA
                     GILINGRLFQGDGGGAGEIGHVVVQENGETCRCGKRGCLETVASVRAVLRQMNADSLE
                     AVSEAFLAGEARAVDIVTKAAHFLGVSLANLIGTLNIQKIVLTGDMTRFGPAWLSEVE
                     ASMRAAALTRMSGATKLELGRLDYRACILGAAAFLLLDDYSLLFNEENL"
     gene            22989..24365
                     /locus_tag="PL000_05095"
     CDS             22989..24365
                     /locus_tag="PL000_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC88301.1"
                     /translation="MTFQLIPPRVVPPLDEGFRPAVLANRFFYQAATSPLLLGLERNG
                     GEVSRFETKILPPDHPDFEDNFLYVERIVKFLLWQRGGHTLHVGNSPRVAEYLQTLYS
                     AQGERKFDWEFMGEQVYERPLKILACALDEVPASRETGKLVGRNLQGCRIGFDLGASD
                     RKVSAVVDGTPIYSEEVIWEPRKNSDPEYHYREIVTAIRTAASKMPRVDAIGGSSAGI
                     YIDNRPMVASLFRSVPRERFGEIKEMFSKIQKEMNVPLEVINDGDVTALAGSMSIEDD
                     GILGIALGSSEAAGYVNMEGHIMGWLNELAFAPIDYSPSAPVEEWSGDMGCGSSYFSQ
                     QCVFRLAPKAGIDIPSEVTDAEKLKFVQEKLEAGHEGAVNIWRSMGIYLGYGIAHYAD
                     FYDMKHVLILGRCTSGRGGDLLIEGAKKVFEVEFPELLQKVELHLPDEKIRRVGQSVA
                     AASLPVIN"
     gene            24397..25248
                     /locus_tag="PL000_05100"
     CDS             24397..25248
                     /locus_tag="PL000_05100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC88302.1"
                     /translation="MKFHLDTAEIFVPDLEPVEKALARTTHLCLAAHQDDIEIMAAQP
                     IIECFQQKEKWFTGVVVTDGRGSPRNSLYENYTDDEMRLVRFKEQRKAAVVGEFAAQV
                     MLDIPSKVIKDVSRTEPVEDILKLLRATRPRYVYTHNLADKHDTHVGVALRVIAALRM
                     LDQSERPERIVGCEVWRALDWMVDSDKVLMDVSNHENLQAALLGVFDSQIVGGKRYDL
                     ASMGRRRANATYFESHGVDATTGLSYAMDMTPLMNDAKMDPAEFAQEFIQRFAVDVKS
                     RIGRMNA"
     gene            25245..26282
                     /locus_tag="PL000_05105"
     CDS             25245..26282
                     /locus_tag="PL000_05105"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC88303.1"
                     /translation="MTLFSEINEQPERIKALLASQRKNVERIAAEIKKRDIEYVFLAA
                     RGTSDNAGRYANYLLGAMNGLPLALATPSLFTYYKRPPKLKNALVIGVSQSGKSPDIV
                     SVLEEGRKQGCQTLSITNEPDSPLAQASDFVLDIHAGTEKAVAATKTYTTELMSVAML
                     SAALSGNKAQWNDLSKVAAWMRQSLKQNDFLAQAAQRYRYIDQTVVLGRGFNYATAFE
                     WALKLKELTYIIAEPYSSADFAHGPIAMVESGYPVFAVAPKGKVFNSMLEMLNRLRSD
                     IAAELIVISNDKRALALAQVPLSIPVDVPEWLSPLVNILPAQLFAYHLTVAKGYNTEQ
                     PRSIRKVTETK"
     gene            26433..27404
                     /locus_tag="PL000_05110"
     CDS             26433..27404
                     /locus_tag="PL000_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88304.1"
                     /translation="MRVCVLSDEIIDDFNPSPYLKDYDWEMVTLTAPVEEKIRALSES
                     KKFDVFLNVCEGYEFEDEEDHEQGYQAIEVVEALEKLGLPFTGADSNCFDPTREEMQA
                     IAEAHGVSFVKGYHVTGEDEARELVKNLRYPIMVKHPKSYGSTGMTRESRAETLEQVL
                     AQVKRICSEFGAARMEEFIVGKEYNVLVVENANDLSQPFAYPPAELVFPPNEDFWHVD
                     VKWNYDVPFDFNQVKDPALIEKLHDIAKRMYLAMGVSGYGRCDIRMNDQGELFILEIN
                     PNGGIMFKPEEYGPADYMILYDPDGYKGFFDRIFQAALARQKMRSKK"
     gene            complement(27493..28257)
                     /locus_tag="PL000_05115"
     CDS             complement(27493..28257)
                     /locus_tag="PL000_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88305.1"
                     /translation="MNKNTRIMVSSVAAIFVLACACPATSLPTINTQPTTPPFVVPPT
                     QAIPPTQDGFEVPTLPPLEDTNILMSDDFNEFSSRIETTSDETGSSEIKDGVYILRAN
                     GDVWNWGKSNSEFSDTVIEVDASMTVGPANNNAGIGIICRMQTREDTSIDGYLLAISA
                     DGYYSIRSILAGSMEPLVDWTYSDTIVQGSGHNIIRATCDGNSLILEVNGEQVATAAT
                     ISGGPESGPMAFSVVSFESAEPTVEVHFDNLVVTAP"
     gene            complement(28306..28716)
                     /locus_tag="PL000_05120"
     CDS             complement(28306..28716)
                     /locus_tag="PL000_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88306.1"
                     /translation="MENHEFDFWLGEWDVTWGEGVHGTNRVESILDGAVIQENFNGDG
                     LIGISVSVFSREDSRWHQTWVDNSGSYLDFVGEFVDGKMILSRNGIAEGKPIKQRMVW
                     FDISANQFRWNWERSDDDGANWRALWEIQYQRKA"
     gene            28859..29815
                     /locus_tag="PL000_05125"
     CDS             28859..29815
                     /locus_tag="PL000_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88307.1"
                     /translation="MRVCILSDEAIEDYNPSVFFRAHEWDFFTVTPPVSDFIRQIVEQ
                     KNYDVFLNICEGADDNDADSGFSVVQTLESLGVPFTGADSKFYNPTREQMQTAAEARG
                     LNFARGFNARSVQDLSQAHALRFPLIVKHPNSFGSAGLTPHSRVDSFEELEEQFQRNF
                     SEYGSARVEEFIEGREVSCLVADNPDDLANPFAYLPVEVKFPVGETFMHMEVKWLNWD
                     TFIVPLEGEGLVRSVQEVSRQMFQVMGGTGYARVDIRVRPNGELVILEINPNCGILYY
                     GPDDRSMSDLPISWDKDGHDGFLDRIFRSAILRRDLRSGREK"
     gene            complement(29833..29997)
                     /locus_tag="PL000_05130"
     CDS             complement(29833..29997)
                     /locus_tag="PL000_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88308.1"
                     /translation="MINKRRASTIFLILGLVFLVIGIGMDNTTFSYAAIAFVLISLVL
                     GGRWLRPRKR"
     gene            complement(29994..31406)
                     /locus_tag="PL000_05135"
     CDS             complement(29994..31406)
                     /locus_tag="PL000_05135"
                     /inference="COORDINATES: protein motif:HMM:NF015368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88309.1"
                     /translation="MRPFEIVIPILLAIYLFWPNPRPLLIRLAPAFALAATLIHFTFE
                     GYRWQMIPLYVLSPILAASGLIKFTKVDSPSFSSVWKERIGRGLTLGLLAVSTVIPAL
                     LPVPQIPTPSGSLKIGTVIYEFTDTSRTELYSDDKSEPRRFMVQAWYPATPQKSDQHA
                     QWMSNAETFAPAISGYLNLPSFFLDHLALVNVPAYENAQAAKTDTGYPIIVFSHGWNG
                     FNAQNTGQALELASRGYVVIAVQHTYGAVVTVFPDGTVAPNNPQALPEDANDPNYEVV
                     ARKLVNQWAGDISFTLDQFAGQNQDSESIFFQTLDLSRVGVYGHSTGGGAAIQFCGTD
                     PRCKTILGMDPFMRPVSEEVLQNGLSQPSFFMFSQGWADVTDSKNNQLFNQFQPNLGD
                     NRGIIVISGTKHFDFSDLPLLSPIAPQLGLKGPLNGGRVVEIVNSYLLGFFEMTLKNQ
                     PSELFNGNFADFNEVKERNQ"
     gene            31474..>32720
                     /locus_tag="PL000_05140"
     CDS             31474..>32720
                     /locus_tag="PL000_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88310.1"
                     /translation="MILFSLSIFISAFLLFQIQPMIGKLILPWFGGTPAVWSTAMLFF
                     QVLLTGGYAYAYWLVKRPKQGWIHLAVLGGTIAILTTLGLVWSSPITPPANIHFAMTG
                     LPVLDVFLILTASVGLPYFVLAANGPLMQAWYSRIFPERSYARLYALSNIGSLLGLLA
                     YPLFVEPAFSVFYQGWGWAIVFFVFSVIVGILSWRTRNESEFGRNALRTETPALALHV
                     LWILLSGTASLFLLSVTNQVSQEVAVIPFLWILPLGVYLLSFILAFSDSRWYERRIYA
                     LLFSLVSVAMLWVLTKTESLNVLVQIIVYNLLLFLACMICHGELYRLRPQADHLTSFY
                     LMVSLGGAAGGLFVNLAAPYLFSGYWEFYLGWLLVVVFLVALLWPGLHSRMERQAQIM
                     VAAFVLGAVLLALGLNREDGPLFV"
     assembly_gap    32721..32820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32821..33495
                     /locus_tag="PL000_05145"
     CDS             <32821..33495
                     /locus_tag="PL000_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNC88311.1"
                     /translation="PTTYYVEDSGVGLLLLNHPRRGHGLRVGVLGLGAGTLAAYGETG
                     DLYRFYEINPTVVQLAEGAGGYFSFLSDSRAEVVTVTGDARISLEQELAKGQRQDFDV
                     LVLDTFSSDSIPVHLVTREAFALYLEHLAKDGVIAAHISNRHLDLRPILWLMAQEYDL
                     SMIQIDRPASGGDNGFPSSWVLLARDSSLLEIPAIKSHSADFESYVTAIKPWTDDYSN
                     LFQILK"
     gene            complement(33597..34358)
                     /locus_tag="PL000_05150"
     CDS             complement(33597..34358)
                     /locus_tag="PL000_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HNC88312.1"
                     /translation="MFFGFDLKEILMFPFKDQESRKYFLIGCLVALAAFFIPIVPYLV
                     LFGYAVRIARQVMNNESPRMIAWDDWGGMVKDGVKVFGIRLILVSPILILLIPLIISS
                     IAFPIVMNSVNSSEIESVATVFSLLVFASLCCIIPLSIPLGLIIPGAEMHVIEKDDFM
                     AGLRFKEWWPIFRANLSGFIAALAITYVVSMAVTLVIQILGATLILACLVPFLLLGMT
                     LHLTILMFVLGAQAYKVGKEKLAQQPEDPAPAPAE"
     gene            complement(34393..35031)
                     /locus_tag="PL000_05155"
     CDS             complement(34393..35031)
                     /locus_tag="PL000_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNC88313.1"
                     /translation="MLKKLREPVNSLTHWGGAILALAGLIALLIVGWSTPAKIISLTI
                     YGVSLIAMFSASATYHMVRVKDKALEIFRKIDHAAIFLLIAGTYTPFCINAFTGFWKW
                     GMMSIVWSLALIGIGVKIFYIRSPRWLNAGIYVLMGWISVIAGGQMLAALPTWVFVWL
                     LIGGVVYTLGAVVYATKIFNFIPGVFGFHEVWHIFVLLAAAAHFVAVMGVAL"
     gene            complement(35088..35600)
                     /locus_tag="PL000_05160"
     CDS             complement(35088..35600)
                     /locus_tag="PL000_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88314.1"
                     /translation="MENEQQQVLRKEALGNNVIRIIGILSFLFTLFSCAGAVMLDLVG
                     QKAIGKVSNAAVNCPPGKTCWTGRVDFTTEQGEQVTFHPLTAPMLFDFDPFLSGRSYA
                     DYGNYQVRYFAPFPKFAKVKLAYFLEYSTQVTGCCFGVFMLLIASAFSTKPGKPHKPL
                     VIDLSKFRNK"
     gene            complement(35653..36270)
                     /locus_tag="PL000_05165"
     CDS             complement(35653..36270)
                     /locus_tag="PL000_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC88315.1"
                     /translation="MLMKNFMRVFFHLLYHPFAFTYDLVAAVVSFGQWKNWGRSILPF
                     IEGTRILELGHGPGHLQRFLLDLKLDPVAIDESAQMGKLARKRIGPTHKLTRGLAQTL
                     PFQTASFDCVISTFPTEYIFDPRTLSEIKRILKPAGRLVVVPAAFPSNRFLKWLYKVT
                     GENPQALDDNLKKRLVAPFTQIGFLTEVQIKEVKSSRVVILVAKK"
     gene            complement(36325..36681)
                     /locus_tag="PL000_05170"
     CDS             complement(36325..36681)
                     /locus_tag="PL000_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNC88316.1"
                     /translation="MSDRDEFGAFLVGFVVGGLTGAVVALLFAPQSGEETRALIKDKS
                     IELRDKAQMSAEEAYARAEQLAADARARAEELSKEARARAAELASEVRERGKGALEAV
                     RSKAGKKADGSEAAAG"
     gene            complement(36717..37178)
                     /locus_tag="PL000_05175"
     CDS             complement(36717..37178)
                     /locus_tag="PL000_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88317.1"
                     /translation="MATLPPTEQPTPPPLTPEQLAEIREQERKQKQMMYGIVAVIVLL
                     VALLGVAIYFLLQPNTPTDKIRDVFIIVVALETLVIGVALVVLIVQLASLINLLQNEV
                     RPILHATSETVNTLRGTAEFLGENVVEPVIKLNGYLAGLKRMLELMGIKPK"
     gene            complement(37235..37861)
                     /locus_tag="PL000_05180"
     CDS             complement(37235..37861)
                     /locus_tag="PL000_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88318.1"
                     /translation="MPNSFSIRPAVQADTLALSRLVIQLYAAELPGALTGPIDGQEAL
                     FRFTLEANGENALKNRYVMCDEKGNILATGMIQFSDEPTFERAPDGTVSNAFRLLGTP
                     AALKLFLTVARSLIGVQKHEDMESALIHSVVVDQNLRGKGIGVQLMTHLEERICEHGV
                     KKSRLQVLEDNISAVPFYQKLGYKQISKSPWWVSLLSWSSLGMEKNLA"
     gene            complement(37864..41043)
                     /locus_tag="PL000_05185"
     CDS             complement(37864..41043)
                     /locus_tag="PL000_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNC88319.1"
                     /translation="MISRIRAALNLREGEERTVFLMLAQYFFMGAAMLFVQSASFALF
                     FTAWDTTAMPYIYLGIAVIVSSITALFLKISARTSLAQFLILSILFLLFGTIALRIGL
                     AITTSKWLMLILPIWSQTLVNISVTAFWTLAGNLFDVRQGKRIFGLMNAGSWLAYVAM
                     GPFTTPLVNAIGTENLYTVIALCVLISFVLQQITLKKNPRTYATPESPEGETQKTSIL
                     HYLRNKYIFLIFALITTWRVSYFILDNIFYDRAALRYPSTAELAGFIGGFFGLVGLLG
                     FVTDMFLTGRIISRFGLRAGLLATPALTVLCTAALAVTGSINPNLILVLFWLAVAGKF
                     ANEGVGFSLDQTASALLYQPIAESDRIRAQTITEGIVQPLAIGLAGLLLLLFNTILKF
                     DAIQLSFIYLLSGTIWIGICLALIRAYPNALTEALHKHRFGDKGIPLADLVSKEIVIN
                     ALKSPHPNEALYALELLEKSGSENFTEILVQQLRSPYPEVRIYAAKSIERLKINEALS
                     EIEVLFQKDAVPNVRQIAACTWSALSKNAKDALKLVNSPDTNTSHGVVVGLLRTRKIN
                     EVQEAYQHLRSLAGSEHAHEKLQAANLLVEIANPQMVEVLTPLLQDESIEVKKAALRA
                     AAKTHHPDVYPLVITALASPQTRSLAFNALVAGRETALPEIIRSIKDESLHRRIHLRL
                     VRACAHIKGDAVHVLESWINHHNVSLRSRVLTALHECRYKPEGRSMQIVQNQVEVELR
                     RAAWLLSCLGDLQSTPKTEAIIRAFNDDLAETRDRLFYLFGFLHDPHIIHRARSAIAR
                     NDENRTAYAIEVMDATLSQSHKAAFIPLLENFPAAERLQKMGTRYKQDKPRTSRIIDI
                     LTNALAHENPWLVANAIEIAREVGIAESQVIIESLTGSKESLIVRIARQKRTEKNMLS
                     TVERVIILKSLRMFADTPDEALAELADLLQEMSVMPGETVIKEGETGESLYIIVDGKM
                     EVVDDNRVLNQLGARAVFGELSLLDSSPRSATVRALEETSLLRLDQTPFYEIMSDYVE
                     VAMGTIHMLTRNLRARTGDVLELSRILGQ"
     gene            complement(41155..41886)
                     /locus_tag="PL000_05190"
     CDS             complement(41155..41886)
                     /locus_tag="PL000_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC88320.1"
                     /translation="MNHKKFLQVFILLAVLVSSLGFTSGVSAYSCGTSYTVQSGETLS
                     SIAQTCGTTVAALQLANPKANPYIFVGQILVLPGAFWDNGNGYATYVVSRGDTLKSLA
                     ARFGTSMDTLASLNSIANYNLIYEGQRMTVPSGVAVPVPPPAAGGTYTVQQGDTLKKI
                     ATRINVTVNDILLVNPQITDANLIYVGQVISLPASASYYTVQSGDTMKKIAARFGTTL
                     DSLIVLNPQITDINKIYVGQVIRIW"
     gene            42024..42584
                     /locus_tag="PL000_05195"
     CDS             42024..42584
                     /locus_tag="PL000_05195"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC88321.1"
                     /translation="MTRDDVLDAAAQVFRQKGFHGASMSDIASALDVQKASLYHHVTS
                     KQEILLALLERALGMLTEHIASFAARTIPADQKLRLMIRGYLSALAENADLTSVLLFE
                     HRSLDKKSHTRHVPQRDRFEGLWHDVLDEGVREKVFDLKDTGLAVRAIMGTLNWTLTW
                     YRPDGPKSIEQIADEYSDFILKGLLK"
     gene            42625..>42881
                     /locus_tag="PL000_05200"
     CDS             42625..>42881
                     /locus_tag="PL000_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC88322.1"
                     /translation="MYSFEPNEEQQMLIDAVGKFASNDLRPAAHDAEESHEMPQKLIN
                     KGWELGLLQASTPEAYGGFGERSAVTTVLAVEEMAFGDLAGA"
BASE COUNT         9909 a        10431 c        11669 g        10672 t
ORIGIN      
        1 taatgttttg gtttctgatt tctgggttac tggtttctga atcttatccc gcccgcgaag
       61 cgaggcggga atttttttct gtgaaaatgg gtgcgtcgca cctcggataa acaaaacagc
      121 gcggaagcct ccccaaagac ctccgcgcca ctatccactt tctaatttcc acttttcact
      181 ccccactttc tttctgctca ctacttcttc gcccgttcct tcgccgcctt caacaacccc
      241 acaaacagcg gatgcggccg catgggacgg gacaaaaact cagggtggaa ttgacttgcc
      301 accatgtatg ggtggtctga aatttcaacg atctcaacca gtctgctgtc gggtgagaga
      361 ccagagaaaa ccatgccatg tttttcaaaa tcagcgcggt aggaattgtt gaattcaaag
      421 cggtggcgat gacgctcgtc cactttggcg accccgtaag cggtggcggc cttcgtccct
      481 ttttggagca ggcacgggta aagtccgagg cgcatggttc cgcccatgtc ggtgatcgag
      541 cgttggtcga tcatcaggtc aatgatgggg tgttccgttc cgcggtcgaa ttcggatgag
      601 ttggaatctt cgaggttgag tacatggcgg gcgaagtcaa tgcacatcac ctgcatcccc
      661 aaacacagac cgaggtacgg caccttgttt tcgcgggcga agcgtgcggc catcaccttg
      721 ccttcaatgc cgcgcgaacc gaaaccgcca ggcaccagca ccgcatccga ttttttgacg
      781 atgtcccagc ccttgtcttt ttcaaggtcg gctgagtgga cccagttgat ttccacttcc
      841 acgtcgttgg cgagagcggc atgtttcagc gcctcacgca ccgacatgta ggcatcctgc
      901 aattcgacgt atttgcccac gagagcaacc ttcacggtcg gttttggctt gcgcacatcc
      961 tcaaccagtt ttttccacgg gcgcatgttg ggcttgcgtt ttgccttcag cccaagtttt
     1021 tcaaggaata gttcgcccac accgagcttt tcgagcagca ggggaacttc atacaaaacg
     1081 tcggctgttt tcatggggat gactgcatct ttttccacat cgcagaaaag tgcaatcttt
     1141 tcgcatagac ctttgtccac gtcctggtcc gcacgggcaa tgatgatgtt cggcgaaatg
     1201 ccgatggaac gtaacgcggc aacagaatgt tgggtgggtt tggtcttgat ctcaccagtg
     1261 gcgccgatgg tggggagcca ggtcacatgc acgaacaggc agttctcgcg gcccacttcg
     1321 ttgcggagct ggcgcagggc ctcaaggaac ggctgcgatt caatatcacc gaccgtgccg
     1381 cccacttcga ggatcatgat ctcggcgcct gtctcttttg cggcgagccc gatgcggcgc
     1441 ttgatctcgt tggtgatgtg cggaatgact tgaatcgttc cgccgaggaa atcaccgcgg
     1501 cgttccttgg cgatggtttc cgcatagacc tgccctgtgg tgaaattgct gactctggtc
     1561 aggcggttgt caatgaatct ttcgtagtga cccagatcca ggtcggtttc tgcgccgtca
     1621 tccagcacgt acacttcgcc atgttggtat ggcgacatcg tgccagggtc aacgttgata
     1681 tacgggtcca gcttttgtac ggcgacattg aagccgcgtt ccttgagcag caaacccatt
     1741 gcggcagcgg tgacgccctt gcctacagat gaaacaacac cgccggtgaa aaataaatat
     1801 ttagtggtca tgatttttcc ttttctcgtc cttctcccat ggtgtggggg tgaggttaac
     1861 aaagagaatg gggagggctt taagccctcc ccattctggg gttacgaaca atttgggtga
     1921 tgaaatttct ttcggctcat ccgaccagct ttacgaggtc ttccgcggcg cgccgcattt
     1981 cgaggaagat cataccgagt tttccgtcag gacgggacat ggcggtgagc acggcttcat
     2041 cgccgatggc ggtgagcaca atggcgccct tatcgccttt gatgtaaacc tgatccagcc
     2101 cgccgcggcc caattcgctc gaaattcgct cgccaaggct taacatagcc gccgacatgg
     2161 ccgaaacacg atcttcttcc acaccctgct gcaacgcaga tgccataata aggccgtcga
     2221 cggatacgac ggcagatgct tcgatatcgg gggcggctgc ctgcatattt cgcaggcgtt
     2281 ccaccatctg gtcagaacgt gattttgcca taactcagtc tcctgggccg ttttgtgatt
     2341 gattggctcg tagtgtacca gaaaaataat tattcgagaa ttgcttgcac atttcaataa
     2401 tgtcattgca aggcagcggg taaaaaatgc cctgatttgg gtcttcacct cgcaaattca
     2461 caacaagatt tgaccctgcc cctccttcat gttaaacttg ccgattgcca tgctgaaact
     2521 gcccgtcatt ttcgcgctct ttttttggat gcaatccacc accgcggcca taacttcgcc
     2581 gcaacccggg gataccctgc gcgggcaggt ggaaattctg ggaacgatgg atgtgccgaa
     2641 ctttgcctcg gcggaactgg cattttccta cgacgccccc agccccgccg aaagctggtt
     2701 caccatccag acattccctc agcctgtgca aaaccccacc ctcgccgttt gggatacgac
     2761 caccgttacc gatggagatt acaccctgcg tttgcgcgtc ttccttacgg atggttcctc
     2821 acaggatgtg gtcgtttcgg ggttgaagat ccgcaatgat gagccgctca caactgcaac
     2881 gccgacctcc accgctcagg cagatatcca gctttttgaa accgcagccc ctcccacgtt
     2941 gacgccccgt ccgcagccaa ctttgcaaac cttcccttca ccgacgcctt tgccgcccaa
     3001 ccccgcctcg gtgacctcct cttcgatcta tgtcaacttc gggcgcggcg cattgatcgt
     3061 gttggttttg ttcatttttg tcgggatcat cttcagattc aggagcaggg catgacccgt
     3121 ccgcgtgctt gtgcggccat tgttcaaaac aaaaaaattc tgatggtcct gcatcgttcc
     3181 tcttcaagag aatattggac cctgcccggc ggcggaattg agccaggcga aacgcccgaa
     3241 caaaccgctg tccgcgaggt gaaagaagag acaggtcttg atgtgacgat gggaaattta
     3301 cttttctttg aaccatctgg tgaatattgc ttttcggcgg aattggcaaa cnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacaggtatc gaaagtgatc tccgcacttg
     3481 gaattgaatt atgacagaca ccacttatct catcatcggt ctcggtaacc cgggccgcga
     3541 atacaaagac acaaggcaca acatcggctt catgttggtg gaccagcttg cggtgcggtt
     3601 ggatgcgcgc ggaatgaaat tgcaatccaa ggccatcgtc atctctgctc atcacgaaga
     3661 gcggaagatc atccttgcga agccgcagac gtacatgaac ctttcaggcc aatcggtgca
     3721 gggacttctc cacttttaca agatccccat cgaaaacctg ttgatcgctc acgatgacat
     3781 ggatattccc ttcggcacga tacgcattcg cccgggtggc gggcctggcg gtcagcgcgg
     3841 aatggcatcc accattgatc atctcggcac gaaagaattt ccgcgtttgc gcctcggcat
     3901 tggccgcccc cccggccgca tggacccgaa agactacgtt ctgcaaatgt tctcaaagga
     3961 ggatatgaaa ctgctgcccg atgttctcag ccgtggagca gatgctgcgt tggaatttgt
     4021 gctgaagggc ctgaattcag cgatgaataa attcaacgga gaagtgggca agtagggatt
     4081 gaaaagtaaa catatcccag ttcaatttcc acacttcgat ctttctcaaa ccatgcaacc
     4141 ccttctttcc aacatccgca cgcttcctgc ttaccagcaa ttactggttg agcttaacgc
     4201 aggcaaaacc atcgctggcc ttgggctttc gcgttctgcg cggttgcctg tcttggctgc
     4261 cctgcaagcg gacttgaacc gccccatttt gcttgttacc gatcgcgccg atcatgcgct
     4321 ttccattttt gacgaacttg atttctgggt taaatcaccg cgtctgttgt tcgcggaacc
     4381 gaatccgctt ttttatgaag aagccgcctg gggagtttcc acccgccgtt cacgcttgca
     4441 gaccctgacc acgctctcca cgtatcattt gccattttca aaaaaacctg aaacgccgcc
     4501 gatcatcgtt gcttcggttc gttcgctgat gacacgcacc ctgccgcgcc gtgactatct
     4561 caaagcgtgt aaaaaacttt cggtgaacca gcgcattcaa cccgatggat tgatccgcga
     4621 atgggcgagg atcggttatc agcgggtgaa tactgtgctt gaaccaggtc aattttcaca
     4681 tcgcggcggt ctgctcgata tctggccgcc caccgagttg aatcctgtgc gcctcgattt
     4741 tttcggtgat gaaattgaga ccatccgtca atttgaccct gccacgcagc gcacggtcga
     4801 aaaacttgaa aacatcctca tcacccctgc gcgtgaatac ctacagcccg aaggggaacc
     4861 ctccgaaaac ctgagcgaat ttcatattcc gcttttacat tcgcagcctg cttcggtgct
     4921 cgattatctc ccgccgcaag cgctcgtgtt gatggacgac atcagcctgg ttgagaccat
     4981 ggcaaatgag atcgaagaac aggcggtgaa atttcgaggc gaatgtatcg ccgaaggtac
     5041 gcttgcggcg gctttccctg tgccgtatgt gccgtggtcc gatttgaacg acgacattca
     5101 ccacatgccc tgtcttgagt tgggtcattc cactgaatta ctcgaaagcg aaagcagcgt
     5161 tgaccttgct tcaaatttca gccacgatga acgttttggc ggcaggctta aaccattcat
     5221 tgattacctt gccagcatac ttgaacgcga cgagcaggtt tacattgtct cgcgccagac
     5281 gccgcgcttg cgtgaattgt gggcggaaca ttatccgaac tcagagcctg aaaatctttc
     5341 tttccttgag gcttcgcttt ctgaagggtt cacgtttcaa acctcgcatc tcatcaccga
     5401 ctctgagatc ttcggctggg aacggccgca gccgcgcatt cgccaaaagc aggtagccga
     5461 gacgcccgaa tcgatctatg ctgacttgca aacgggtgac tatgtcgttc atgtagatca
     5521 cggcattgga cgattcaacg gtctgattca gcgacaactg gacggccacg aaagagaata
     5581 cctcaccgtt gaatatgacc gcggtgacat tctctacgtc cccgtccatc aagcagaccg
     5641 actcacgcgt tacgtcggcg cggatggcgg ggcgccctcc cttgaccatc tcggtggaca
     5701 ggcctggcag gaaaccaaag ccaaagtcaa agaggcggtg cagcaggttg ctgaagacct
     5761 cctcgatttg tatgcgcgcc gtcaggttgt ggaaggttat tctttcggcg ccgatacgca
     5821 gtggcaaaaa gaacttgaag acagtttccc ctatgtggaa accgaagacc aaaaacgtgc
     5881 catcctcgat attaagcgcg acatggaacg tgcccgtccc atggatcgcc tgttgtgcgg
     5941 tgatgtgggc tacggtaaaa cggaagttgc tcttcgcgcc gccttcaaag ccgtgatgag
     6001 cggcaagcaa gtggctgtgc tggtgccgac cacggttctg gcgcagcagc atttcgaaac
     6061 cttttcgcaa aggctggcgg cttttcccgt caaagtggaa atgctctcgc gtttccgaac
     6121 ccaccgcgag cagactgaaa ttttgtacaa actcgccctc ggtgaggtgg atattgtcat
     6181 cgggacgcat cgcctgatct cttctgatgt ggagttcaaa gacctcggtc tggtcgtcat
     6241 tgatgaagaa cagcgttttg gtgtcacaca caaggagcat ctcaagaaac tccgcaccga
     6301 agtggatgtg ctgacgctca ctgccacacc gattccgcgc actttataca tggcgttgac
     6361 gggtgtacgc gatatttcca atttgaatac gccgcccgaa gaacgcctgc cgatcgttac
     6421 gcatgtcggg ccatattctc cgcgtttggt gcggcaatcc attctgcgcg aactggagcg
     6481 cggtggacag gttttcttcg tccacaaccg cgtgaacacc attgacgcga tgaaggcaca
     6541 cctcaacaaa ctcgtccccg aagcgcgtgt ggatatcggt cacggtcaaa tgcccgaagg
     6601 tcaactggct gccgtgatgc atcgcttcac cacgggtgaa attgatattt tgctttccac
     6661 gaccatcatc gaatcaggtc ttgatattcc caatgccaac accctcatcg tagatcgtgc
     6721 cgataccttt ggccttgccc aactgtatca actgcgcgga cgggtcggcc gcggcgcgat
     6781 gcgggcctat gcttacttct tccgccacaa caaaatgtca ccgacacagg aaggccagca
     6841 aaggcttgag gtcattgctg aaaataccca actgggcgca ggctactcca ttgccatgcg
     6901 cgatctggaa atccgcggcg caggcgaatt gctcggtaca cgccaacatg gatacatcca
     6961 gtcggttggt tttcatcttt acacacgaat gctcgccgat tcggtccgcc gaattcgagt
     7021 gttgatgaaa gatggattga atatcgatgt gccatctgcg cttttgcctt ctgccttcag
     7081 cctgccgctt tccatgccag taaatgtgga tctgcctgtt gccgttggta ttcccagcga
     7141 ttacatctcc gatcaggatc tgcgcctgcg tttgtatcgc cgcatcgccg acttgcgcga
     7201 tgaaaccgaa ctcgatgcgc tcggctctga attccgcgac cgttttggtc atttgcctga
     7261 aatggttcaa aacctgctgt atcaaatgcg ggtgaaactc cgcgctgaaa aggcaggact
     7321 ttcttcggtg agctgggaag cggggcagat cctgctcaag taccccgcac ccgctgaagg
     7381 cgtagagccg aagcgtctgc ctgatttggg cgaaggtgtc cgtggcggca aatccgctta
     7441 ttgggtgacg gtcgcaaaag aagaagcctg gaccatccga ttgcttgatg tgttggatcg
     7501 tttgctgaaa agccccgcct cttgatttcc cgaggcccga ttctgtttca ataaaaattc
     7561 ctgccccgac aatttgtcgg ggcaggcttg cttactggta gttgctgcaa ttcagatcct
     7621 ctttcaattt cagcagcgtg tcatcgcgct cttgtgatgc gggcgtgtcg agcgccagcc
     7681 gatccagcat gccgcataga ctgggctcca gccccgccgt gatgcgcttc gcagacttgg
     7741 tcaaggtttt gacctgctcc caatcttcaa gatgaccata gccttcgata aacggaatga
     7801 tctcgaagcc gttatcggcc tgctcgccaa tggcttgtga ttctttccaa agtttcacaa
     7861 ccgagtccca atctttgtat tgacgggcga ggtcggcttt ttcgaaaaag taacaccatg
     7921 tgtcggtgtt ttgttttccg tagatttgtt ctggcgggct tttctctccc tgctgaatca
     7981 attccacgtc cgagcctgcg ctgagttgac gcatgtccgt gctgatcaat cgcaggttgg
     8041 tgtcttcggg ccgcagcacc cacaggcatc tttgcatttc agggttgaag gtgaacagga
     8101 tcatcttgtc gctgtttccg ttgaattcca tcgtcaactt gctgtcttca agcggaatcc
     8161 ctgccagaaa atcgttcaca ttggggttgg tgtaataaaa agggaaatac caatggggcg
     8221 gtgtttgaat atcgcctggc tggtaggtgg tgatgatcga atacgagacc gagtagctgc
     8281 ccatgtagcc gaggatttct tcatcagtga ccagggccgt gcctgcttcg atcttcggtg
     8341 cgcgccagag gagctgctgt gcaaaacgct cctgtttttc ccaggaatag gcaaaatcct
     8401 tttcgttggt cagatgcatc gagatcgcca gcgcaatgca tgccgaaacg accacaatct
     8461 tttttctccc gtcgatcagg cggtcgatca acacagccag aatcaatgct gcgcccagtg
     8521 tagagccaat tccgaaacgt gtccctgcga attgattctt gtgcgtcacg atatcctgcc
     8581 cgatgaccca gatgggggcc atgccaagca ggagcagaaa caggcccaag gtcaacggct
     8641 gcgcgaacca gctgccacgc tcctcttttt cggtgatctg tttcaggtgg atgaacaccg
     8701 ccacgaaggc aaaaatcgaa acaaggatta cgaaccaact aaaaatggaa ctgaacgcaa
     8761 acaactctgg cacgagagtc cgataccagc ttgtaaatgt aatgatgacc gagtcttgca
     8821 atgcgattcg aatcaggtag gcaatggttg gcaggggctt gctaaacaac gcgaacaaaa
     8881 cttcaggcgc gttgcggtct ccgatgggcg gcggcacata caggaacatc cgccagatga
     8941 tgtaagccgc aagcgcagcg agataaggaa tccactgttt gatggcggtc atgatgcgct
     9001 ggcgggttgg gagttcgggc gaatttagaa cccaatagag gatcagcggg cgcaccatca
     9061 ccatgccgac aaaatattca ctggtgaaga gatgaactgc ttcgagaaaa accgccagca
     9121 actttaacga aatgcgatgc ttggactctg tggcattccg tgccatgatg agcaaactgg
     9181 ccgagaaaaa taaaaaccct gcccaataca aaatcgaatt gaccgcatac ggctggatca
     9241 aaaagaaagg atggacaatg aaaaggtagg tcgcaaaggt cacctgcttg ttgtggcttg
     9301 gccaaattcg gcgaagcaaa taccaaagca gtgtgcccgt caaccagcga aagatcaccg
     9361 ccgccagatg ccaggcgatc ggtttgaatc ccagcaggtt gaacatgacg atgtacagcc
     9421 acgctgcatt cgggcggctg tcgtaataca attccagcgt caggctcgga atgcctttgt
     9481 tgaacgcata aaagatatac ggccaatcat cctgatagaa gccgagtcga ttcgcaagca
     9541 aaccgaaacc gataaaggca acaacgagaa acgccagcgg cgtgtatttt tcctgactta
     9601 agatgttttg taaaacggat cggattcttt tcatgacggc gtgattatac tttcactccc
     9661 gcctcaaccc tgaccttgaa cctgtctctg tttcactggc ctttcccccg cctttgcagt
     9721 aaaatagaac actcgttccc ctaatcttca agaccccata taccccaaaa aaacatggac
     9781 ttcaaactaa ccgctccctt tatccccatg ggtgatcaac ccgaagccat ccgcggactt
     9841 gtggatggtg tgcgcaaagg catgaaacac caggttctgc tcggcgccac tggcacgggt
     9901 aaaaccttca cgatcgcctc cgtcattcag gaattgcaga aacctgccct tatcatggcg
     9961 cacaacaaga cccttgcagc gcagttgtat gctgagttca aggaattctt ccctgaaaac
    10021 gcggtgtcgt atttcgtttc gtactacgat tactaccagc ctgaggctta cgtcccgcgc
    10081 catgacctgt tcatcgaaaa ggaaacccag atcaacgaag agatcgagcg cctgcgcctt
    10141 gcagccacca ctgcattggt ctcccgccgc gatgtcatta ttgtggcttc ggtctcatgt
    10201 atttatggtt tggggaatcc tgaagagttc aacaagggtt ctgtgaattt ggcagtgggg
    10261 cagttggtcc gcagaaatgc attgctgcgc cgtttgatcg aatcgcagta ccagcgcaac
    10321 gacatggaac tgcgtacagg caccttccgc gtgcgcggcg atacgctgga gatcatccct
    10381 gcttatgagg aaaaacgtgc ctaccgcatc accttctttg gtgatgaagt ggaacgtatc
    10441 atgcagttca gcccgctgac aggcgaagtg ttggaagagc caagggaaat ttccatttat
    10501 cccgccaagc aatatcttac cgacgccgaa aagaccaaag aagcgatggc ggatattgaa
    10561 tcggagttgg aggaacgact ggcgcaatac aaacaggctg gccgaatact cgaggcacag
    10621 cggcttgagc aacgcacccg ctacgacctg gaaatgctcc gcgaagttgg gtattgctca
    10681 ggaattgaaa actattcccg tcacttcgat cgccgtgccg ccggcaccca cccgtggaca
    10741 atgatcgact ttctgccaag tgattatctg ctggtgttgg acgagagtca catgaccgtg
    10801 ccgcagatcc gcggcatgta caacggtgac cgcgcacgca aggaaacttt ggtggagtac
    10861 ggcttccgcc tgccctccgc aatggataac cgtccgctca agtttgatga atttgaagag
    10921 gtgatgggtt caacgatcta cacatcggcc acgccttcag cttacgaaat ggatcacgct
    10981 gagcaggttg ttgagcagat cattcgcccc actggtttga tcgaccccga agtggatgtc
    11041 cgccccactc gagggcaggt ggatgacctg gtcggagaga tcaagcaacg ggtcgagcga
    11101 cgcgaacgta tcctggtcac gaccctcacg aagcgcatgg cagaggatct gaccacgtac
    11161 atgaaagaac tgggaatcaa agtccagtat ttgcattcgg aagtggaaac gttggagcgt
    11221 gtgagcattc tgcgtgacct gcgcctgggg accttcgatg tgctggtggg aatcaacttg
    11281 ttgcgcgagg gccttgacct gcccgaagtt tcactggtgg cagtgttgga tgccgacaag
    11341 gaagggtttc tgcgttcaga cacggcgttg atccagacca tcgggcgtgc ggcccgtaat
    11401 gtcaacgggc gcgttattat gtacgcagac cgtatgaccg attcaatgaa gcgcgccatc
    11461 gacgagacga atcgccgccg tgccaagcaa atgaaataca acgaagaaaa tggtattgtg
    11521 ccgatcagta ttcacaaaga aattcatgac ctgaccgagg aaatgtcggc aaaggcagtt
    11581 ggcgaaatga agggtgagta caaagtgaaa ggttcgcagg gcatgccgcg cggcgaactt
    11641 cggaagatcg ttgacgaagt ggaaaagcaa atgaaggaag ccgcaaagaa ccttgaattc
    11701 gaaagagccg cggcgttgcg tgatgaactg tatgagttga aaaccctgct ggctgaagat
    11761 gaaagcttga aaccgtggga gcgtatcaaa ctgatgactg gtgaggacga ttagttctgg
    11821 aaataaaacc agccgcctcg ttacaacggg acggctggtt tttgttttag tcagtgggtc
    11881 taacgggacg gctttggttt gtgatggata caggggtaaa gttctccatc cacgatcaaa
    11941 atgtgattga cgatccagtc tgatatttct tcgtggatct tcaaggccat ttcatttgag
    12001 ccgcccgatt gggtgaattc ggcatggtac ttatcgaatt tctcaacaaa caccgcatga
    12061 gcatgtttat tcttttcggc ggcagggcaa tgataggttt ccatgcagtc ttcttctttt
    12121 ccgaagtgcc agcctgcata aaaccgtaaa acacccagta ttctggcgag ttcctcgccg
    12181 ccgtgacctt cgttgatcac gtcgccaagc ttgttgaggg tgtcaaccag atagcggtgc
    12241 tgactgtcga gtgaagcatc cccggtgctg agtgcgttgg tccattccag atgtcttggt
    12301 gagtacattt tgcctccatg gcttcatgta acaaggatta taaatacatt ctggtttaca
    12361 taccttgcaa gctctgctta attttgtttg gttctagctt atcctgaacc tctggctatc
    12421 agaaaagtaa atgtcaggcc tggtcgttgc cttcctcttc gtcatcgggt tgaatatcgt
    12481 caaatggctg gagaagtttg agcgcccttt tcacttcatc cttggggatg aagatatcca
    12541 cacgtgcggt ggcaagggtg taggcagtac gagaaatgga ttcctcgacc agttccacgt
    12601 cgattccctc ggcttcaaga aagctctcga taacttccgc ttctgcgcgg ctgttggctt
    12661 caccgatctt ttcgtaggtt agctcgtcca ttttggcggt ctcctgatta aagtatatgc
    12721 caatttagct gttttgtttc atcgaaagcg cgcgtcctat gatcctggca gatgtgtttg
    12781 cggcggccgt aaacccaaag aacggaccaa aatcctgtgc ggcgggcaga cctgtaaaat
    12841 acaggttcgg aacgctgctt tggaaggatt cgtccaatac ggggaatccg ttttgggttt
    12901 ttattttggc ggccaaggtt ccgttggcga gcatggggat ttgcgcaatg tccattttgt
    12961 agcccgtggc aaggatgaca tggtcaatgg ggaaggttga accgtcattc agggtaattt
    13021 gcaattctcc cgcaggcagt tctttgcagt cggctacctg agtttgagga tgcaggtgga
    13081 tggtctcctg agtgatacgt ttggcgagcc atggctccag tttcagcctg ccttcgcccc
    13141 acatccgttt gatgatggcg tctttttctt cctgtgcgag attgcggaac cattcggggc
    13201 ggtcagccat ggattcaacc agtggattga cccatgtcca gtctgagggg gtgaaggcgg
    13261 gtgtgtcgtg gcggtaggtc atgtgaatgt tctgtgcgcc ctcctcgtgg atcaaggctg
    13321 cccattcaaa ggcgctctgc ctgccgccaa caataagcac tcgtttgcct ttcaggttgc
    13381 taaagtctat atattcggca gtgtgatgaa acctgccaga agggaatagc gaattcaacg
    13441 gttcggggat gtttttgaaa taacgcaagc cgaccgccat gacgagcttt tctgcgagtg
    13501 tcgaactgcc atctgcaaaa gtgacgtcaa agccattgcc ggcctggtcg attttcgtga
    13561 tccatgcggg tagtggggaa atgccttttt gacgctgaaa ccattcgatg taggtcaggt
    13621 aaaagtcgag gctgagcggt tccacatccg ctggttgaag gttgagcgtt ttcaaatagt
    13681 tgacaatggt ggcttcgttg tgcggatcaa ggtgccagtc gcaggcggag cgaaggaaca
    13741 ttttgagggg catgaaattc ttccagtagt ccatgcttgc gccaacatga atgtgttcga
    13801 tcttttcatg ctttaaatag gctgccagcg agaggccaaa tgggccagcg cctgccaaaa
    13861 gtaaaggggt gtttttttgc atatctgcct ccgtttggga cgattttttc agagataagg
    13921 cttattgtac tctttgtgat ttctttatag agtctttaca ttggggttgt atcctgtggt
    13981 tgtaatcaaa tcaaataagc ggaagaattc cgcagaaaac aggagataaa aatgaaaaag
    14041 acaattttga tcgtaggttt gctggttttg gcgctgggcg tgttgggagt aggtgtggtc
    14101 tttgcgcaag ggacgaaccc tccgtacgcg ggtcacggcc cgatgcgaaa cggtgaaggc
    14161 cctcttcata ccttcatggt gatcgagttc gccaagaagc ttgatctgaa cgtgaacgat
    14221 atcaacaccc gcattgccgc tggcgagacc atgtacgaca ttgccctttc ggctggtgta
    14281 actgccgaag aattccccac agtcatgacg gaagtccgca cggctgcgct ggatgcggct
    14341 gtgaaagcca atgtcattac gcaggaacag gctgactgga tgaagtctcg tggatttgga
    14401 cagggcggca tgggaactgg tacctgtgac ggcactggca tgcagggtgc tcaaggacag
    14461 ggcatggggc gcggtatgat gggtcaggga cgcggctggc agaaccaaca ggttgctccg
    14521 taatacatcg aataaatcaa aaaggagtcg gctgcggctg actccttttt gattcttgtt
    14581 ggcttggagc ttattctttt cctgtcagta aatacttttg cacatcgccg cctgtgaaga
    14641 ttccgtctgt gacaacttcg tagccgccga gctctttgat ctcatcgtac atggctgagg
    14701 ctttcgcaag cagcgagcgg aagatgtatg gaccgaagct gacgcggttg atcccgatct
    14761 cctgtaattg tttcaggctg gcggtctttg cagaggcgag gacgttgatc ggggcgtgga
    14821 tgcgctggcg caggatcttg agcgtttttt cgtcaccagc gccgatgggg tagatgcaat
    14881 ttgcgcctgc tttcaggtag gcatttgcgc gggtgacagc atcttcgatg cgctcttctg
    14941 tggaggcaaa accctctgca aagaagctgt ctgtgcgggc gttgatgacg agggagattc
    15001 cgcgtttttc ggcagtttct cggatggctt gaatgcgttc gacctgttca ggaatggggc
    15061 ggagacgggc gccgtcatcc cagctgtctt caaagttgat gcccacagcc cctgtatcaa
    15121 gcagattgga aatgttttgc tgtaatcccg ccacggtttc ggagtaacct gcttcaaaat
    15181 cagcagtgac agggacctct acgctgtttg cgatcctcct gaccatttcc agcatcgatt
    15241 ccagtttgag ttgttcgcca tcgttgtagc cgagagattc ggcaatggcg gcgctggcgg
    15301 ttgcgacggc tggaaagcct tttacctcca acatccgcgc gccgatggga ttccatacgt
    15361 tggggaggat cagtaatttg ccgttgttgt gcaaggcaag cagggattga gctttggcgg
    15421 cctgggtcat ggggtctcct taatggtggg cgtggtgatg ttcggcgatg ctgcaaaaca
    15481 ggtcgctgga ttgacagcct tcacattcca gttgcagtgt ggcgtgttgg atgtggtatt
    15541 cgtgggcaag catttcgttg atgttgtttt gaatgaaggt tccatcgcgg agtgatatct
    15601 catcggtcac tacatgggcg gaaagcatgc gcaggccttc gctgatgctc cagacgtgca
    15661 ggtcgtgtac atcgcgcaca ccatggactt tgcgaatgtc tgagatcatg gtcggcatgt
    15721 cgatgttttc gggggtgctt tccaaaagga tgtggatggt ctgccgcaaa attccccaag
    15781 cgttccagac aatgaaagca ccgatcaaaa cgctgacaaa tgggtcgagc cagttccatt
    15841 ttgtgaagta gataaccacc cctgcaatga ccgcgccaag tgtggaaatg acatcgccca
    15901 tcaagtgcag gaatgcgctg cgcaggttga gatcgtgctc acttcctttt tgcacgagcc
    15961 aggcggtgac ggcgttgatg aaaaatgcca gggcgccgac tcccatcaga atcgctgaat
    16021 caacttcggg cggggaaagg aagcgctgcc aggcttcgta gaaaattcca agggcgatca
    16081 atatcaaagt ggtggaattg accagcgcaa cgagaatccc gactcgatga tagccgaagg
    16141 tcttgcctgc attggcgggt ttgagtgcga tcttgagcgc gtaccaactc aagccaagag
    16201 cgatgacgtc ggtgaagtta tgggcggcat cggtgagcag ggcaaggctg ttgccgaaaa
    16261 ttccagccgc cacctcaacg aggacgaaag cggcggtcag tgaaagggaa agcgacaatc
    16321 gttttgtagt ttgcgtggca aggtcgccga agtgtgaatg attgtgcgaa tgtgacatgc
    16381 tgtaattata gtagagacac ggcagcgccg tgtctctttt tttatgccca gccaaacaat
    16441 ctggcaatcg aggcggtgag gaaggtgacc agaaacgctg tgcccaaagt catcacgacg
    16501 ctgagggtgg cccatttgag cgacttggtt tctttgtaga tcgtcatgat ggtcgtggcg
    16561 catggattgt gcagcaacgc gaacagcatg aggttgactg cggtcagcat tgtccagccg
    16621 ttgccgtgaa cgagcagatc gtaaatggct gtgtttgaag aaggcccgtc gatcatcatg
    16681 cctgctccca tgtacatcat catcatggtc ggcacaacga tttcgttggc agggatggca
    16741 atgatgtatg ccagcagaat gacaccatca agaccgagca gcaagccaaa tggattgagc
    16801 caatcggcaa tggcacgggc gaggctggta tcgccaatgt gaacgtttgc caaaatccag
    16861 atgattgcgc cagcaggggc ggcggtttgc atggcgcgcc acaacacaaa aatggtacgg
    16921 tcgatgatgg atgtgtagag gatgcgccca aagttcggtt tgcggtaggg cggcagttca
    16981 agcgtaaaag cggatgcttc ccctttgagg acggtgcggg acaggatcca cgacatgagg
    17041 aaggtgaaca caaccccgag taacaccaca ccgaccactg atcctgctgc gatgacggat
    17101 gaggtcaacc cgctgaagcc tgccgccaca aaaaccgtac caagcatgat gagtgtgggg
    17161 aagcgaccgt tgcatgggac gaagttgttt gtgaggatgg cgatcaaccg ttcacgcgga
    17221 gagtcgatca cccgtgttgc gatgacgcct gccgcattgc agccaaatcc cattgccatg
    17281 gtcagcgatt gcttgccatg cgctcccgcc tttttgaaca tccaatccat gttgaaagcc
    17341 acgcggggaa gatagccgag gtcttcaagg aaggtaaagg ctgggaagaa aatcgccatt
    17401 ggcgggagca tcacactcac cacccaggcc aatccgagat agacaccatc ccaaatgaag
    17461 cctgtgatcc accaggggat gaatcccagt gaattgaaaa aattgcgccc gatttctcca
    17521 aaagcgaaca aaacatttgc aatggcatcg gaggctacgt ttgcgcctga aacggtcagc
    17581 cagatgatca gggaaagcag aatcaacatg atcggcagcc ccgtgatcgg cgaggtgacg
    17641 aggcggtcta ttttttggtc gaggtcatag cgcttgttgt ccgcagtttt tacggcgcgt
    17701 tttgcaatgg cctctgcttc agagtacagc gatttgacga tctcatcgcg aaaccccgat
    17761 gagagattgt tacggagtga ttccgctttt gagagaatgt catttggttt taattcggta
    17821 gccatattac tgatttcctt ccacagacat tttcctgctg aactgcacgt ccatgcgctg
    17881 ctggcgcgag acgatatcgg ccagttcgcc gctggtcaat gctttttgta cgcgggcatc
    17941 tccatcaagc aagcggatgg ccagccagcg tgcattcggt attcctggtg caacctgttc
    18001 gatcatgggc accagttcgt ttacggcttt ttgaaactcg ggtgtgccgt tcacgcgcaa
    18061 gggtttgatc tcgaccttgc cttcgatcac ttcagctaca acgccgagca gggtgtgtat
    18121 gccttccccg gtgcgggccg tgatcggaat cgcaggcacg ccaaggtcac ggctgagaga
    18181 acgtgtatcc acttcaagac ctttgcggcg ggcttcgtcc atcaaattga ccgcaacgac
    18241 aacgttctcg gtgatttcca tgacctgcat gacaaggttt aagtttcttt ccaacgcggt
    18301 ggcgtcagtt acaacgatcg tgcagtttgg ctggccgaac aggatgaaat cacgggccac
    18361 ttcctcatcc tgcgaggcag ataaaagcga gtatgtgcct ggcaggtcta ccagtttgta
    18421 gcgcatgcgg ttgaattcaa aaccgccctc tgcacgggtg acggtttttc caggccagtt
    18481 gcctgtgtgc tgtttgagcc ctgtcagcgc attgaataaa gtggatttgc ccgtgttcgg
    18541 gttgcccgca agtgcgatca cgcggtcgaa atctcccact ttaattccca tttgttccaa
    18601 ctgtgtaaag gcggggcagg tttcgcaatg ttctgtgggg agtacagttt ttgtgtttgt
    18661 catggtttct cttttctaaa atatgcactt cttaaagcgg cttcacccag atctgtgcag
    18721 cctgatcttt gcgcaatgcg atcaaagtgc cgcggatgcg ataggcgcgc ggatcgccga
    18781 aaaaattttt taattcaggg tagatcagtg tgccgggggt cagaccaagg tccaggaatc
    18841 tgcggcgcgt aaaaccttgc acagcatcat cgagcatcac gatctcgccg cgttggtcgt
    18901 gtgcaagttc tgccagcggc acggcttcgc ttttcagcga ttcgatctct ggcagtgcgg
    18961 tcacatgcac attcgaagcc acggcaggtg ccagtttgta ctcggtctcg ccgtcactca
    19021 gcagatagcg gccttgtgtt ttttcaataa tgcggatgac ttgtcccagg cggagccctg
    19081 cggccagaat ctgttgatac gcaatggcag gttcatcttc aagatgtacg attcttgcgg
    19141 gacgatcggc ctgccaggcc gtaatgggca ttccctccgc cttaggcaga gtcccctcac
    19201 gagtcggaat ggggtcgccg tgtgggtcgc gggtcgggtg gccgagtgca gcatcaagct
    19261 cgttgagttg ggcatcggtc agggtatgtt cgcggcgctg ggcttcatcg tggactttct
    19321 ccaaaggcat tcgcgcttca tctgcgagaa atctctccca aagcctgtgc gcgcggacga
    19381 catgcaaggc ccagcgttct cctttggagg tcagatgcag gtcattgccg cgggtttcaa
    19441 gcaattcctg tgtttccata ttttcgatca gctgggtggt ttttgccagc ggcaggtcaa
    19501 gtgtccctgc aagagaaacg ggggaggcgt gtcgtccctg ttgttcacgg tcaagcagat
    19561 gcttgagagc atcttccacg tgttcacgtt ctttggcttc gcggtaggtt ttgtacacgg
    19621 cgatcaaccc cacgcgcggt agaaagatca acgcgctcaa ggcagcccca aggatcaaaa
    19681 ggatgatcgg attcatgatg gtttacctcc aagctttggt agaaaacgac ctgtggtttt
    19741 catatattca cgatattcat cgccgaattt tgcaatgagg ttggcctcct cttttggcgt
    19801 acgaattgcc atgacaatga atgccagaac tcccaaaaga gcgatgaacc aattgtcggc
    19861 catcaatcca aacgaaatga ataacgatgt cccaactgtg tacagcggat gccgaaccca
    19921 gcgatatggt ccgcttgtgg aaagtttatg ttccttgcgg gtggcgctgg caggggaaat
    19981 gccgtcgcca atgctgctaa aaagccaata aattagtccg tcactcgcaa caccaagccc
    20041 caccccaagc cagcgcgccc attcgggcag gccgatcttt gaccaagcca tccattgcgg
    20101 ggcgatcagg tacacaaacg ggcttagcca taaaagcagg ccgaacagtt tgatgattcc
    20161 catcatgaca gatccatctg ttttgcggga aaccttctcg cctgtgtgac ggtcggcttt
    20221 gatgcggtga taacttgaga tggccatgcc gctgatcaat acaattgcgg ctatccctcg
    20281 gaaaatggtt tcattcatat ttttttacct ctatttctaa atggtcaaat ttctttcacg
    20341 tagatcttgc ttgtaatgtt caacccaagc acaatggatt ctttttcgat ctggatggtc
    20401 aggttgtggt cgtagggaga gtacgccttg attttgattt cagccccagg gaccagtccc
    20461 agattctcga gataacgcag caggtcggta tctgctgctt tgacacattg aatggcaacg
    20521 atacattctg gccgcagtga agagagcgga gtcgaatctt caaagggcat ttcaagatcg
    20581 gcggtgggga tcagctctcc gtgtgggtca cgaagcgggt gtcccatggc ggcggcgatc
    20641 cgcctttcga aatcctcaga aatgacatgc tcaagctttt cggcttcttc atggacctca
    20701 tcccatgtgt acccgagggt ttgaaccagc cacgcttcca acaagcggtg atgacgaatg
    20761 acttccaatg cggctttttt gccgtcgtcg gtcagggtga cgccctggtg tttttggtag
    20821 gtcacaaggg cgggtttggc tgaagcaagt ttttgcacca tgccagttac agaagctggc
    20881 ttgaccttga gtttggcggc cagtgcattt gtgctggcgc tggtgccgtt ttcagtgagt
    20941 tcataaatgt gcttgagata atcttgaatg gagatggtca ggttttgttc catgttatgc
    21001 gttccagagt gtgatggtgg tgcaggtgaa aaagatgaaa tacgccacgt tgaatcccgc
    21061 tccttcaagc gcgtctgtga taaaagcagg caaaatacgc ttggaaagca gccaggcgat
    21121 ggtcatggtt acggggttga tcgccgccat ccatgcgggg aacattgtgc tttgtgtggc
    21181 tactgcaaca gaaaataaaa ctgatgcgat cacgaccata accagcaaag gcgcgtaggt
    21241 cacgacgagt actttgcggt gacgggtgaa taattccaca aaaatatttt gcgaatcttc
    21301 gctgacctcg ttcagggctt tgacatattc acctgcgtaa aagaaagacc catgtacaaa
    21361 ggcaccgatc acgctgctgc agacaaatag cagggcagtg gcagttttga ggatcgggtt
    21421 tatatccgcc agaccttgat agacctgcca aaacccagca agcaccaatg gcgtagagaa
    21481 gaccccgatc agtgcgcccc acatcatttt ttcagatggg atggaaacga acttctcggc
    21541 atctgaaagt aatttttgaa aaggttttag tttgggatag tcgtcgatgt tcaccttgtt
    21601 tgcaaggagt aaaacatctc caatggcata caaggttgcg ccgacaattg ccaacaaacc
    21661 tgtgacttgt attgaattca taaatactcc taaaattagt caagcctaaa tatattttag
    21721 gcttgactaa tttatttgtc aagggcaggg ttgacttttc gtgggtgaat ctgtacaatg
    21781 cccttagttt gtccattgaa tttacaaagt cctttgcctg gattttcaca cggataacca
    21841 ttgaaaaaag cgacgcatca acaaacgaag cagcacaatc gcgacctggt cctgcgcacc
    21901 atatttttgc aggagagcat cagccgcgct gatattgcac gcctcacgaa cctgacccgc
    21961 accacggttt cagatgtagt ggctggtctg atcgccgaag gtttggtttg tgaagtgggg
    22021 catggtgctt ccatcggcgg caaatcgccg atcctgctga gtgtgatcgc cgatgcacgg
    22081 tatgtcgttg ggttgaattt ggcgcaggat aaattcatcg gtgcgcttgt caatttgaag
    22141 ggggagatca aggaaaccat cgaattggat gtacatgaaa gcgatggtga aaaaggcctg
    22201 caatttgtgt atcaaatcat ggatcagctc ttcaaaaaga aattgaaacc catcattggc
    22261 attggcattg gcacaccagg gttggtaaac acccgcgagg gcgtggtgct caacgctgtc
    22321 aatctcgatt ggcaggatct gcctctgggt gctttgcttg aaaaaaaata caaggttccc
    22381 gtctcagtat taaatgacag ccaggcggct gccataggag agtttgtgta tggcggcgat
    22441 catagacctg atgagaattt gatcgttgtc aacgcacggt atggcattgg ggcgggcatt
    22501 ctaattaatg gacgattgtt tcaaggggat ggcggtggtg caggtgagat cgggcatgtt
    22561 gtcgtacagg agaacggtga aacctgtcgg tgtgggaagc gcggctgcct tgaaaccgtt
    22621 gccagtgtcc gcgcggtgtt gcggcaaatg aatgctgatt cgctggaggc ggtgagtgaa
    22681 gcgtttctcg ctggtgaggc gcgtgcagtg gatatcgtaa ccaaggctgc gcattttctc
    22741 ggtgtttccc ttgccaacct gatcggcacc ttgaatattc aaaagatcgt cctcactggc
    22801 gatatgaccc gttttggccc tgcatggctt tctgaagttg aggcgtccat gcgtgcggcg
    22861 gcattgactc gcatgtcagg tgccaccaaa cttgaacttg gcagactgga ttatcgcgcc
    22921 tgcattctgg gcgctgcagc gtttctgttg ttagatgatt attcactttt gttcaacgag
    22981 gaaaacttat gacctttcaa ttgatccccc cgcgtgttgt cccgcctttg gatgaaggtt
    23041 ttcggcctgc cgtgctggca aatcggtttt tttatcaggc ggcaacttcc cctttgcttt
    23101 tggggttgga gcgtaatggc ggcgaagtct cgcgttttga aacgaagatc ctcccgcctg
    23161 accacccaga ttttgaggat aattttttgt acgtcgaacg cattgtcaag tttttgcttt
    23221 ggcagcgcgg cgggcataca ttgcatgtcg gcaactctcc acgggtggct gaatatctgc
    23281 aaacgcttta ttccgcacag ggtgagcgta aattcgattg ggagttcatg ggtgagcagg
    23341 tgtatgagcg gcctttgaaa attcttgcat gtgcattgga tgaagtgccc gcttcgcgcg
    23401 aaacggggaa actggtcggc cgtaatttgc aggggtgccg cattgggttt gatcttggtg
    23461 cctccgaccg caaagtcagc gccgtggtgg atggaacacc catttacagc gaagaggtca
    23521 tttgggagcc gcgaaaaaat tccgatcccg aatatcacta tcgcgaaatt gtgactgcca
    23581 tccgaaccgc cgccagcaaa atgccccgcg tggatgcgat cggcggcagt tcggctggga
    23641 tttacatcga caaccgccca atggttgctt cgttgttccg cagtgttccg cgtgaacgat
    23701 ttggcgagat caaggagatg ttttccaaaa tccaaaaaga aatgaatgtc ccgttggaag
    23761 ttatcaacga tggcgatgtg accgcactgg caggctccat gtccattgaa gatgacggga
    23821 ttttgggcat tgccctcggt tccagtgagg ctgcaggtta cgtgaatatg gaaggtcata
    23881 tcatggggtg gctgaacgaa ctggcttttg cgccgatcga ttacagcccg agcgcacccg
    23941 tggaagaatg gtcaggtgac atgggttgcg gttcctcata tttttcacag cagtgtgtat
    24001 ttcgccttgc gccaaaagct ggcattgaca ttccctccga agtgaccgat gccgagaagt
    24061 tgaagttcgt gcaggaaaaa ctggaggcag ggcatgaggg cgctgtcaac atctggcgga
    24121 gtatgggcat ttaccttgga tatggcattg cccactatgc tgatttttac gacatgaagc
    24181 acgtgttgat tctgggacgc tgcacctcag gccgtggcgg tgacctgctt atcgaaggcg
    24241 caaagaaggt cttcgaggtt gaattccctg agttgttgca gaaagtggaa ctgcatttgc
    24301 ccgatgagaa aatccgccgt gtggggcagt ctgtggctgc ggctagtttg cctgtgatta
    24361 attgatatca tttcagctat ccaataaagg agtcctatga aatttcatct tgatactgcc
    24421 gaaatcttcg tccctgacct tgagcctgtg gaaaaggcgc tggctcgcac aacacacctg
    24481 tgccttgccg cgcatcaaga cgacattgag atcatggctg cccagccgat catcgaatgt
    24541 tttcagcaga aggaaaaatg gttcacaggc gtggtcgtga ccgatggccg cggttctccg
    24601 cgcaacagcc tgtacgaaaa ctacaccgac gatgaaatgc gccttgtccg cttcaaggag
    24661 cagcgcaaag cggctgtggt gggcgagttt gccgcgcagg tgatgcttga tattcccagc
    24721 aaggttatca aggatgtctc gcgcaccgaa cctgtggagg atattctcaa gttgcttcgg
    24781 gcaaccagac ccagatacgt ctacacccat aaccttgccg ataaacacga tacacatgtg
    24841 ggagtggctc tcagggtgat cgctgccctt cgaatgctgg accagtccga acgtccagaa
    24901 agaatcgtgg ggtgtgaggt ctggcgcgca ctggattgga tggtcgattc cgacaaggtg
    24961 ttgatggatg tctccaatca tgaaaatttg caggccgctt tgttgggtgt gttcgattcg
    25021 cagatcgtgg ggggcaaacg atatgacctc gcttcgatgg gtcgccgccg tgccaatgcc
    25081 acgtattttg aatcgcacgg tgtggacgcc acaactggcc tttcctacgc aatggatatg
    25141 accccgttga tgaacgatgc aaagatggat cctgcagaat tcgcccagga attcattcaa
    25201 cggttcgcag tggatgtgaa aagccgcatc ggcaggatga acgcatgaca ctattttctg
    25261 aaatcaacga acagcctgaa agaatcaagg cgcttttggc ttcgcagcgg aaaaacgtgg
    25321 agcggatcgc tgctgagatc aaaaaacgcg atatcgagta tgtgttcctt gcagcacgcg
    25381 gtacctccga taatgcaggg cgctatgcaa actatttgct tggggcaatg aacggtcttc
    25441 cgcttgcgct ggctacgcct tccttgttca cctattacaa gcgcccgccg aagttgaaaa
    25501 atgcgctggt gatcggtgtg tcacaatcgg gtaagtcccc ggatattgtc agcgttttgg
    25561 aggaaggcag aaaacagggt tgccaaaccc tttccattac caatgagccc gattcgccgt
    25621 tggcgcaggc ctcggatttt gttttggata ttcacgcagg tactgaaaaa gcggtggcgg
    25681 caacgaagac ctacactaca gagttgatgt ctgtggcgat gctttctgcg gcattgagcg
    25741 gaaacaaggc tcagtggaat gacttgagca aggtggcggc ctggatgagg caatcgctaa
    25801 agcaaaatga ttttctcgct caagctgcgc agcgttaccg ctacattgac cagaccgttg
    25861 tccttggccg cggattcaac tatgccactg cattcgagtg ggcgttgaaa ctcaaagagt
    25921 tgacctacat cattgcggaa ccatattcct ctgcggattt tgcgcacggc ccgatcgcga
    25981 tggtggaaag cggatatcct gtttttgcag tggcgccaaa agggaaggtg ttcaattcca
    26041 tgcttgaaat gctcaaccgt ctgcgctcgg atattgccgc cgagttgatc gtcatctcga
    26101 acgataagcg ggcattggca ctggcgcagg ttccgttaag cattccagtg gatgtgcctg
    26161 aatggctttc gccgttggtg aatattttgc cagcacagtt gtttgcctat catctgacgg
    26221 tggcgaaagg ctacaacaca gagcagccgc gcagtattcg taaggtgact gagacaaagt
    26281 aatgcaagga cctggggggg aggtcaaccc gtggaagaca tgatgcatct tcggaaagaa
    26341 aatttcgaga tgtgtcatgt ctttttttgt aaaaacattg tatgaatact ggttctatat
    26401 gataatgaca aaaaaaacga agaggtgacc tcatgcgtgt gtgtgtactt tcggatgaaa
    26461 ttatcgatga cttcaaccca tctccctatt tgaaagatta cgattgggaa atggtcaccc
    26521 tgaccgcgcc cgtggaagaa aaaatacgcg cgctctctga aagtaaaaaa tttgatgttt
    26581 ttttgaacgt ctgcgagggg tatgagtttg aagatgaaga ggatcatgag cagggctatc
    26641 aggccatcga agtggtggaa gctctggaga aactgggtct gccgtttaca ggggctgatt
    26701 caaattgctt tgacccgacc cgtgaagaaa tgcaggccat tgccgaggcg catggcgtca
    26761 gttttgtgaa agggtaccat gttacgggtg aggatgaagc gcgggaattg gtcaaaaatc
    26821 tgcgctatcc catcatggtc aagcatccaa aaagctatgg cagcacaggc atgacccgcg
    26881 aatcgcgcgc cgagaccctt gagcaggttc tggcgcaggt gaagcggatc tgttccgagt
    26941 ttggtgcggc tcgcatggag gagttcattg tcggcaagga atacaatgtg ctggttgttg
    27001 aaaatgcaaa tgacctcagc cagccatttg cctacccgcc tgcagagttg gtcttccctc
    27061 ccaatgaaga tttctggcat gtggatgtga aatggaatta cgatgtcccc tttgatttca
    27121 atcaagtgaa agaccctgca ctcattgaaa aactgcacga tattgccaag cggatgtatc
    27181 ttgcgatggg agtttctgga tatggccgtt gcgatatccg catgaatgat cagggagagt
    27241 tgttcattct tgagatcaac cccaatggcg gcatcatgtt taagcccgaa gaatacggcc
    27301 ctgctgatta catgatcctg tatgatccag atggatacaa aggttttttt gaccgaatat
    27361 ttcaggcggc attggctcgc cagaaaatgc gttcaaaaaa gtaaccttga gaaaaaaatg
    27421 aaaggcaggc aaacgcaata ttgcaatgtt gcgtttgcct gcctttttga attgttgttc
    27481 aggctgtcac gatcatggcg ctgtgacaac caaattgtcg aagtggacct ccaccgttgg
    27541 ctcggctgac tcgaaggaaa caactgaaaa tgccattggg cctgattcgg ggccgccaga
    27601 gatggttgcc gctgtggcta cctgttcgcc gttgacttcc aaaatcaagc tgttcccgtc
    27661 acaagtggcg cggatgatgt tatgtccgct tccttgaacg atcgtgtccg aataggtcca
    27721 gtccaccagc ggttccatcg aacctgccag gatgctgcga atggaatagt aaccgtctgc
    27781 gctaatggca agaaggtacc cgtcaatgct tgtgtcttcg cgggtttgca ttctgcagat
    27841 gatcccaatc cctgcattgt tattggctgg gccaaccgtc atggaagcat ctacttcgat
    27901 gacggtgtcg ctgaattccg agttggactt accccagttc cacacatcgc cattggctcg
    27961 caaaatgtac accccatcct tgatctcgga tgaacccgtt tcgtcactgg tcgtttcgat
    28021 gcggctgcta aattcgttga agtcatcact catcaagatg ttggtgtctt ccagaggcgg
    28081 cagggttgga acttcgaagc cgtcttgtgt tggggggatg gcttgagtgg gtggcaccac
    28141 aaagggtggc gtggtgggtt gggtattgat tgtgggcagg ctggtggccg gacaggcaca
    28201 ggcaagcaca aaaatggctg caacactgct gaccataatg cgggtatttt tattcatctc
    28261 attctccttg ggtttcccgc attataggtg actcgaaaaa taagcctaag ccttacgttg
    28321 gtactgtatc tcccagagcg cgcgccagtt tgcgccatca tcatcggagc gttcccagtt
    28381 ccatcgaaat tgattcgctg agatatcgaa ccagaccatg cgttgtttga tcggttttcc
    28441 ttccgcgatc ccgttgcggc tgaggatcat tttcccgtcc acgaattcac cgacgaagtc
    28501 gagataactg cccgagttat ccacccaggt ctgatgccaa cgcgaatctt ccctgctgaa
    28561 aacggatacg ctgatcccga tcaatccatc cccgttgaaa ttttcctgaa taacggcgcc
    28621 atccagaatg ctttccacgc gatttgtgcc atgcactcct tcgccccagg tcacgtccca
    28681 ttcaccaagc cagaaatcga attcgtggtt ttccataaaa ctcctccaat tgatttggtt
    28741 tgtaattttt cgagtgtatt gaattctttt acatctgaca acctaccaga gaatgagatt
    28801 tcaagtacga caggagcgaa tataatggga ctaccagttt ataagtgagt gaggccttat
    28861 gcgagtttgc atcttaagtg acgaagccat tgaagactac aatccatctg tgttctttcg
    28921 tgcccatgag tgggattttt ttacagtaac accgcccgtg agtgatttca tcaggcagat
    28981 tgtggagcaa aaaaactacg atgtcttttt gaatatttgc gaaggcgcag atgacaacga
    29041 tgcagattcg ggcttttccg tagtgcagac tttggaatcg ctgggcgtgc cttttactgg
    29101 agccgattca aagttctata accccactcg tgaacagatg caaaccgccg ccgaagcacg
    29161 cgggctgaat tttgcccgcg gtttcaacgc caggtctgtg caagatttga gccaggcgca
    29221 tgcattgcgg tttccattga tcgtcaaaca ccccaacagt tttggcagtg caggtttgac
    29281 cccgcactca cgcgtggact ctttcgaaga attggaagaa cagtttcaga gaaatttttc
    29341 cgagtacgga tcggcgcggg tggaggaatt tatcgaaggt cgggaggttt catgtttggt
    29401 ggccgataat cccgacgatc tggcgaatcc gtttgcatac ctgcctgttg aggtcaaatt
    29461 ccctgttggc gaaaccttca tgcacatgga ggtcaagtgg ctgaattggg ataccttcat
    29521 cgttcctttg gagggtgaag gccttgtccg ctcagtgcag gaagttagca ggcagatgtt
    29581 tcaagtcatg ggcggaacag ggtatgcacg ggtggatatc cgtgtgaggc caaatggaga
    29641 gcttgtgatt ctggagatca accccaattg cggaatcttg tattacggcc ccgatgatcg
    29701 cagcatgtct gacctgccca tttcgtggga taaagacggt cacgacggat ttctcgaccg
    29761 cattttccgt tctgccattt tgcggcgtga cctgcgttct ggaagggaaa aataataccg
    29821 cttgtgtgtg ggttatcttt tacgcggtcg cagccagcgt ccgcccaata ccagagagat
    29881 caacacaaag gcgatggctg cgtatgaaaa cgttgtattg tccatgccga tgccgatgac
    29941 aagaaacacc aatcctagaa tcaaaaagat ggtcgaagct ctgcgcttgt ttatcattgg
    30001 tttctctcct ttacctcgtt gaaatctgcg aaattcccat tgaataactc tgacggctga
    30061 tttttcaaag tcatttcgaa gaaacccagc aggtacgaat tcacgatctc aaccacacgc
    30121 ccaccgttga gcggaccttt caacccaagc tgcggtgcga tgggggaaag caggggcaaa
    30181 tcgctgaagt caaaatgctt cgttccagaa atgacgatga tgccgcgatt gtcacccaga
    30241 ttcggctgga actggttgaa caactggtta tttttgctgt cggttacatc ggcccagccc
    30301 tgactgaaca taaagaacga cggttgcgat aaaccatttt gaagcacctc ttcagaaacg
    30361 gggcgcatga atgggtccat gccgagaatg gttttgcagc gtggatcggt tccgcagaat
    30421 tgaatcgccg ctccgccgcc tgtggaatgt ccatacacac cgactcggct taggtccaag
    30481 gtttggaaga aaatactttc cgaatcctgg ttctgacctg caaactggtc cagcgtaaaa
    30541 gagatatcgc cagcccattg atttaccagc ttccgcgcca ccacttcgta atttgggtca
    30601 ttggcatctt caggcagggc ttgtgggtta ttgggggcaa ctgttccatc aggaaacact
    30661 gtgacaaccg cgccgtaggt gtgctgcact gcgatcacca catacccgcg gcttgccaat
    30721 tcgagtgcct gccccgtgtt ctgggcgttg aagccattcc agccatgcga aaacacaatg
    30781 atggggtagc ccgtatccgt tttggccgct tgcgcatttt cataggcagg gacattcacc
    30841 agcgcaagat gatcgaggaa aaatgaaggc aggttgaggt aacccgaaat ggcaggggcg
    30901 aaggtctccg cgttggacat ccactgagcg tgctggtctg atttttgggg tgtggcaggg
    30961 taccaggcct gaaccataaa acggcggggt tcgctcttgt cgtcagaata tagttctgtc
    31021 cgcgaagtgt cggtgaattc gtagatcact gtgccaattt tgagtgaccc gctgggggtg
    31081 gggatctggg gaacaggcag cagggcaggg attaccgtcg aaacggcgag caggcccagg
    31141 gtgagtccgc gaccgatcct ttccttccaa accgaagaaa aagagggcga gtcaactttg
    31201 gtgaatttga tcaatccgct tgccgcgagg atcggggata aaacgtaaag cgggatcatc
    31261 tgccagcgat agccttcaaa cgtaaagtga atcaaggtgg cggccagtgc aaatgcgggc
    31321 gcaaggcgta tcaaaagcgg acgtggattg ggccagaata aataaatggc aagcagaatt
    31381 ggaatgacga tttcaaatgg acgcatggaa tttcctcgtg aatttattat cccgttttaa
    31441 gcgtggttga atacaataaa acgcggaggc gagatgattt tattttctct gagcattttt
    31501 atttcagctt ttcttttgtt tcagattcag ccgatgatcg ggaaattgat tttaccgtgg
    31561 ttcggcggca cgcctgcggt gtggtctacg gccatgttgt tttttcaagt gctgctcaca
    31621 ggcggatatg cctacgccta ctggctggtg aaacgcccaa aacagggatg gattcatctg
    31681 gccgtgctgg gcgggacgat cgcgattctg acgacccttg ggctggtgtg gtcgtcgccg
    31741 atcactccac ctgcaaacat acactttgcc atgacgggcc tgccagtgtt ggatgttttt
    31801 ttgatcctga cggcctcggt cggtttgccg tattttgtgc tggctgccaa cgggcccttg
    31861 atgcaggcat ggtacagtcg cattttccct gagcggtctt acgcgcgttt gtatgccttg
    31921 tcgaatattg ggtcgctgtt ggggttgctg gcatatccgc tatttgtgga gcctgctttt
    31981 tccgtgtttt accagggatg gggttgggcg atcgtgtttt tcgttttttc ggttattgtg
    32041 ggaatcttaa gctggcgaac ccgcaacgag tctgaatttg gcaggaacgc tctacggact
    32101 gaaacacctg cgcttgcgtt gcatgtcttg tggattcttc tgagtggaac ggcctcgctc
    32161 tttctgcttt ctgtgaccaa ccaggtttcg caggaggtgg cggtgattcc ctttttgtgg
    32221 atcctgccgt tgggggtgta tctgctttcg ttcattctgg ctttttctga ttcgcggtgg
    32281 tatgagcggc gtatctatgc attgctgttt agtttggttt cagtggcaat gctctgggtg
    32341 ttgaccaaaa ccgaatcttt gaacgtgctg gttcagatca ttgtgtataa cctgctgttg
    32401 tttctggctt gcatgatctg ccatggagaa ttgtaccgtt tgcgcccaca agccgatcac
    32461 ctgaccagtt tttacctgat ggtttcgctt ggtggtgcgg cgggcggctt gtttgtgaat
    32521 ttggcggcgc catatctttt tagcgggtat tgggagtttt atctgggttg gctgcttgta
    32581 gtggtttttc tggtggcgct gttgtggcct ggtctgcact cgcgcatgga acggcaggca
    32641 caaataatgg tcgctgcatt cgtgcttggc gctgtcttgc tggcgttggg gttgaatcgt
    32701 gaggatggtc cgctttttgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32821 cccaccacgt attatgtgga ggatagcggt gtggggctgc tcctgctcaa ccatccccgc
    32881 cgtgggcatg gtttgcgggt gggggtgctg ggtttgggtg cagggaccct ggctgcttat
    32941 ggagaaacgg gcgacctcta tcggttttat gagatcaacc caacggtggt gcaactggcc
    33001 gaaggagcag gcgggtattt ttcttttctg agcgacagca gggctgaagt ggtaaccgtg
    33061 acaggtgacg cgcgaatctc tcttgaacag gaattggcca aaggtcagcg gcaggatttt
    33121 gatgtccttg ttttggatac tttcagcagc gattcgatcc ccgttcattt ggtgacgcgt
    33181 gaggcattcg ccttgtacct tgagcacctt gcgaaggatg gggtcattgc cgctcatatt
    33241 tcaaaccgac accttgatct gcgtccgatc ctttggttga tggcgcagga atatgacttg
    33301 agcatgatcc aaattgacag acctgcatca ggcggggaca atggctttcc ttcctcctgg
    33361 gtcttgttgg cacgcgattc gtcgcttctg gagattcctg cgatcaaatc acattcagcc
    33421 gatttcgagt cttatgtcac cgcgatcaaa ccctggacgg atgattacag taatttattc
    33481 cagatactga aataatagcg ggtttgaagt ttgcgtacta tctacacaaa aagcaggtgg
    33541 atgttgcttt ttaacaacat ccacctgctt tgatttcacg atattaactg ccgagtttat
    33601 tccgctggtg ctggagcagg atcttcgggt tgttgagcca gtttttcctt accgacctta
    33661 taagcctgcg cgcccaacac aaacatgagt atcgtgaggt gaagggtcat gccaagcagg
    33721 aggaacggta ccagacaggc aaggatgagt gtggcgccga ggatctggat caccaaagtc
    33781 acagccatgg ataccacata ggtgatcgca agggcggcga tgaatccgct caggttggcg
    33841 cggaatatcg gccaccattc cttgaagcgg aggcccgcca taaagtcatc tttttcgatg
    33901 acgtgcattt ccgcaccggg aatgatcagg cccagtggaa ttgacagcgg aatgatgcag
    33961 cagaggctgg caaatacaag caaagagaaa accgtggcga cagattcgat ttccgagctg
    34021 ttgacggaat tcatcacgat cggaaatgca attgacgaaa tgatgagagg aatgagcaga
    34081 atgagaatcg gagagacgag aatcaggcgg atgccaaata ctttcacccc gtctttgacc
    34141 atgccgcccc agtcgtccca tgcgatcatg cgaggcgact cgttgttcat aacctggcga
    34201 gcgatgcgca cggcgtaacc gaacaaaacc aaatacggca cgatgggaat gaagaatgcc
    34261 gccaatgcaa ccagacagcc tatcaggaaa tatttgcgcg attcctggtc tttgaacggg
    34321 aacatcaaga tttctttcaa gtcaaatccg aaaaacatgg tcaattcctc ctgaaaatat
    34381 tattgcgcga ttttataatg ccacacccat cacagcgaca aaatgcgcgg cggcggccag
    34441 caaaacaaaa atatgccaga cttcatggaa gccgaacaca cccggtataa agttgaatat
    34501 ttttgtagca tacaccacag cgccaagcgt atacaccacg ccgccgatga gcaaccacac
    34561 aaagacccag gttggcaggg cggcgagcat ttgtccgcct gcaatgacgc tgatccaacc
    34621 catcaacaca taaatccccg cattcagcca gcgcggcgag cggatataga agatcttcac
    34681 gccaataccg atcagggcca acgaccagac gatgctcatc atgccccatt tccaaaagcc
    34741 tgtaaaggcg ttgatgcaga atggggtgta ggtgcctgcg atcagaagaa aaatggcggc
    34801 atggtcgatc ttgcggaaga tttccagcgc cttgtctttt acgcgtacca tatgataagt
    34861 ggcactggct gagaacatgg cgatcaggct gaccccataa atggtcagcg aaatgatctt
    34921 ggcaggtgtg ctccagccga cgatgagcag ggcgatcaaa cccgccaggg caaggatggc
    34981 tccaccccaa tgggtgagac tattaacagg ttcacgtaat tttttcaaca taaggctttc
    35041 tccaattttt gttcggctga ttttacttga attcagcgcg gaacaaacta cttgtttctg
    35101 aatttgctta aatcgatcac caaaggtttg tgcggtttgc ctggctttgt ggaaaaggcg
    35161 gacgcgatta agagcatgaa aactccaaaa caacatcccg tcacctgagt actgtattca
    35221 agaaaataag ccagctttac cttcgcaaac tttggaaagg gggcaaagta tctcacttga
    35281 taatttccat aatcggcata cgacctgccg ctgaggaagg gatcaaagtc aaataacatg
    35341 ggggcggtca gtgggtggaa ggttacctgt tcgccctgct cggttgtgaa atctaccctc
    35401 cctgtccagc aggtttttcc tggcgggcag ttgaccgccg cattcgacac cttgccgatg
    35461 gctttttgcc caacgagatc aagcatcacc gcaccagcgc aggaaaacag ggtgaataga
    35521 aaactgagga tgccaattat gcggattacg ttattcccga gcgcttcttt tcgcaagact
    35581 tgttgttgtt cgttttccat ttttaagttc ctttcaggct acttctaaag atggggaaat
    35641 gacggcctgc ctttattttt tggcaaccag gatgaccact cggctggatt ttacctcttt
    35701 gatttgtact tcggttaaaa aaccgatctg tgtaaatggt gcaacaagac gttttttcaa
    35761 attgtcgtca agggcttgcg gattttcacc agtgactttg taaagccatt tcaagaatcg
    35821 gttcgaggga aatgccgctg gcaccaccac cagtctgcct gcgggtttga ggatgcgttt
    35881 gatctcggag agtgttcgag ggtcaaaaat atattctgtg ggaaaggtgg aaatgacaca
    35941 atcgaaggat gcggtttgga atggcagggt ttgggcaagt ccgcgggtta gtttgtgggt
    36001 tgggccaatg cgttttctgg cgagttttcc catctgtgct gattcgtcaa ttgcaacagg
    36061 atcaagtttc aggtccagga ggaagcgttg caaatgcccg ggaccatgac caagctcaag
    36121 gatgcgagtc ccttcgataa atgggagaat gcttcggccc cagtttttcc actgcccaaa
    36181 agaaacgaca gcggcaacaa ggtcgtaggt gaaggcgaat ggatggtaaa gcaggtgaaa
    36241 gaacacccgc atgaaatttt tcataagcat ggataaaaaa agggtttgac tgcgcagtca
    36301 aacccttttc attttgcaaa tgctttagcc tgcggcggct tcgcttccgt cagccttttt
    36361 gccagccttg ctgcggacag cttcgagggc acctttgccg cgttcgcgga cttcactggc
    36421 caattcggcg gcgcgggcgc gggcttcctt gctcagttct tcggcgcggg cgcgggcatc
    36481 agcagcaagc tgttcggcgc gggcgtaggc ttcttcagca gacatctgag ccttgtcacg
    36541 cagttcgatt gacttgtctt tgatcagggc gcgggtttct tcgccagatt gcggagcgaa
    36601 gagcagggca acgacggcgc cagtaaggcc gccaactacg aaacctacga gaaaagctcc
    36661 aaattcatca cggtcagaca tgggaatact cctttgttga ttggttagtt gtttttttat
    36721 ttgggcttga tgcccattaa ttctaacata cgcttcaaac cagccagata gccatttagt
    36781 ttgataacag gctccaccac gttttcgcca aggaattccg ctgtgccgcg cagggtgttg
    36841 acggtttcgc tggtggcatg cagaatgggg cgcacttcat tttgtaacag gttgatgagg
    36901 ctggccaact ggacgatgag gacgaccagt gccacaccga tgaccagtgt ttcaagggcc
    36961 acgacgatga tgaagacatc gcggatcttg tctgtggggg tgttgggttg aagcaggaag
    37021 tagatcgcca cgccaagcag ggcgacaagc agtacaatga cggcaacaat gccgtacatc
    37081 atttgttttt gcttgcgttc ctgttcacgg atttcagcca gttgttcagg agttagggga
    37141 gggggagttg gttgttcagt ggggggaagc gttgccatgt ttttattata gcagattcaa
    37201 gattcgaaaa aaagccaatt ggaaacacat ccattcaggc gaggttcttt tccatgccga
    37261 ggctgctcca ggaaagcaaa gagacccacc agggtgattt cgatatctgt ttgtagccta
    37321 atttttgata gaacggaact gcggatatat tgtcttcgag tacctgcagt cgggattttt
    37381 tgaccccatg ctcgcagatt ctctcttcaa ggtgggtcat caactgcact ccgatccctt
    37441 ttccgcgcag gttttgatct acaacaacgc tgtggatgag ggcactttcc atgtcttcgt
    37501 gtttttgcac accgatgagg ctccgcgcca cggtcaggaa caatttcaaa gctgcaggtg
    37561 tgcccagcag ccgaaatgca ttcgaaaccg ttccatccgg ggcacgttca aaggttggct
    37621 catcactgaa ctggatcatg cctgttgcga ggatgttccc cttttcgtcg cacatcacat
    37681 agcggttttt cagcgcattt tccccgttcg cttcaagggt gaatcgaaaa agtgcttcct
    37741 gtccgtcgat gggacctgtc aacgcaccgg gtaattcggc ggcgtagagt tgtattacca
    37801 gccgtgaaag ggctaaagtg tcagcttgaa cagcggggcg aatggaaaaa gaattgggca
    37861 tggctactgt ccaaggatgc ggctgagttc gagcacatca ccagtgcggg cgcgcagatt
    37921 gcgggtgagc atgtggatcg ttcccatcgc tacttccaca tagtcgctca tgatctcgta
    37981 aaagggtgtc tggtcaaggc gcaggagcga ggtctcttcc aaagcgcgga ctgtggctga
    38041 gcgcggcgaa gaatcgagca gtgaaagctc gccgaaaacc gcccgcgccc ccaactgatt
    38101 taagacacgg ttgtcgtcca ccacttccat ttttccgtcc acgataatgt acaggctttc
    38161 gcctgtttct ccctccttga taacagtttc accaggcatc acactcatct cttgcaggag
    38221 atcggccaat tctgcgagcg cttcatcggg tgtatctgcg aacatgcgta gtgattttag
    38281 gataatgact cgttcgacgg tggatagcat atttttctcc gttctctttt gcctggcaat
    38341 acggacgatc aacgattctt ttgaacccgt cagggattct atgatcacct gactttccgc
    38401 aatccccact tcgcgggcga tctcgatcgc gtttgcaacc agccatgggt tttcatgtgc
    38461 aagggcgttg gtgaggatgt caatgattcg gctggtgcgt ggtttatcct gtttgtagcg
    38521 cgttcccatt ttttgcagtc gctctgcagc ggggaagttt tcaaggagtg ggatgaatgc
    38581 agctttatga gattgtgaaa gtgtggcatc catcacttcg atggcgtagg cggttcggtt
    38641 ttcgtcattg cgcgcgatcg ctgagcgggc acgatggata atgtgcgggt catgtaaaaa
    38701 accgaaaaga taaaacaagc gatcgcgcgt ttcggccaag tcatcgttga aagcgcggat
    38761 gatggcttcc gtctttgggg tggattgcag atcccccagg caggataaca accatgcggc
    38821 gcgtcttaat tcaacctcaa cctgattctg cacgatctgc atggatcgcc cctcgggctt
    38881 gtatctgcac tcgtgcaaag cagtcaacac ccgtgagcgc aagctgacat tgtggtggtt
    38941 gatccagctt tcgaggacat gaaccgcatc gcctttgatg tgcgcacagg cgcggaccaa
    39001 tctcaggtgg atgcggcgat gcaggctttc atccttgatg ctgcggatga tctctggcag
    39061 ggcggtttct cttcctgcaa ccagagcgtt gaatgccaga ctgcgggttt ggggtgaggc
    39121 aagggctgtg atcaccagcg gataaacatc tggatgatgt gtttttgcgg cggcacgtaa
    39181 cgcagctttt tttacttcaa tggattcgtc ttgtaaaagc ggagtcaaga cctcgaccat
    39241 ttgtgggttt gcgatctcaa ccaaaagatt ggcggcttgc agcttttcat gagcatgttc
    39301 cgaccctgca agtgacctca ggtgttgata tgcttcctgc acttcgttga tttttcgtgt
    39361 cctgagaagg ccaactacaa cgccgtgtga agtgttcgtg tcggggctgt tcaccaattt
    39421 caaggcatct tttgcattct tcgacaaggc agaccacgtg caagctgcga tctgccgtac
    39481 gttgggaacg gcatcttttt ggaacaaaac ttcgatctcg ctgagggctt cattgatttt
    39541 caatcgttca atgcttttgg cggcgtagat ccgcacctcg ggatagggag accgaagttg
    39601 ttgaacgaga atctcagtga agttttcact accgcttttt tcaagcaatt ccaatgcgta
    39661 cagtgcttca tttggatgcg ggcttttcaa tgcgttgatg acgatttctt tgctcaccag
    39721 gtcggcaagt ggaatgcctt tatctccaaa gcgatgtttg tgcagggctt cggtcaatgc
    39781 attggggtag gcgcggatca aagcgaggca gatgccgatc caaattgtgc ctgagagaag
    39841 atagatgaat gaaagttgaa tcgcgtcgaa ttttagtata gtgttgaaca gcaggagcaa
    39901 taggcctgcc agaccaatgg caagcggctg aacgatccct tctgtaatgg tttgggcgcg
    39961 aatacggtca ctttcggcga tgggttgata caacaacgcc gaagccgttt ggtcgagcga
    40021 gaagccgacc ccttcgttgg caaatttccc tgctacggcc agccagaaga gaacaaggat
    40081 aaggttcggg ttgatgctgc ccgtcacggc cagggcggca gtgcataaaa ctgtcagcgc
    40141 tggggtggcc aatagacccg cacgcaggcc aaagcgcgag atgattcgcc ccgtcaaaaa
    40201 catgtcggtg acgaaaccga gcaatcccac cagtccaaag aatccgccga taaaccccgc
    40261 cagttcggct gtggaggggt atcgcaatgc ggcgcggtca taaaaaatat tatcgagaat
    40321 gaaatatgaa acgcgccagg tcgtgatgag ggcgaaaatg agaaagatgt atttgttgcg
    40381 aaggtaatgc aggatggatg ttttttgtgt ttcgccttca ggactctcag gggttgcgta
    40441 tgtgcgcgga ttctttttta gtgtgatctg ttgcagaacg aacgaaatca gcacgcagag
    40501 cgcgatgacg gtatacaggt tttctgtgcc gatggcgttg accagcggtg tggtgaatgg
    40561 tcccattgcc acgtaggcaa gccatgagcc tgcattcatt aatccaaaga tgcgttttcc
    40621 ctgccgcaca tcgaaaaggt tgcctgccag agtccaaaat gcggtaacgg aaatgttgac
    40681 cagtgtttga gaccagatgg gcagaatgag catcagccac tttgaagttg tgatggcaag
    40741 cccgattctc agggcgatcg tgccgaacag gaggaaaagt atggagagta tcagaaattg
    40801 cgccaatgaa gtgcgcgcgg aaattttcaa aaacaacgcc gtgatggatg aaacaatgac
    40861 cgcaatgcca aggtaaatat atggcattgc ggttgtgtcc caggctgtga agaacagggc
    40921 gaatgaagct gattgaacga acaacatggc tgcacccatg aaaaaatact gggcgagcat
    40981 gaggaagaca gttcgttctt ccccttcccg aaggttgagg gcggcgcgga tgcgggaaat
    41041 cataaaatgg tttgcaaagt ttaccagcgg gattttcctc cgtccatgat gggggtggaa
    41101 tccaaaagtc ccacgagatc atctcgtggg acttttggat gggcaggtct tcaattacca
    41161 aattcggatg acctggccga catagatctt gttgatatct gtgatttgtg ggttgagtac
    41221 gatgagggag tccagggtcg tgccaaaacg tgccgcgatt tttttcattg tgtcaccaga
    41281 ttggacggtg tagtaggagg cgctggcagg caggctgatg acctgtccaa cgtagatcaa
    41341 attcgcatcg gtgatctgcg ggttgaccag cagaatatcg ttgacggtta cattgatgcg
    41401 ggttgcgatc tttttcaacg tgtcgccttg ttgaacagtg taggttccgc ctgcggcagg
    41461 gggtgggaca ggaactgcaa ctccgctggg tacggtcatg cgctggccct cgtagatcag
    41521 gttgtaattg gcaatgctgt ttaacgaagc cagcgtgtcc atgcttgtgc cgaaacgggc
    41581 tgccagtgat ttcagcgtgt cacctcgtga gacgacgtaa gtagcgtagc cattgccgtt
    41641 atcccaaaat gcaccaggta gtaccaggat ctgtccgaca aaaatgtatg gattggcttt
    41701 ggggtttgca agttgaaggg ccgcaacggt ggttccacac gtttgcgcga tgctgctcaa
    41761 tgtttcgccg ctctgcactg tgtagctggt tccgcagcta taggcagaca ccccgcttgt
    41821 gaatcccagc gaagagacca gtacggcaag aaggataaag acttgtagaa attttttgtg
    41881 attcatgtta cctctcattc attacagtag ttcgatatga atgagacgaa ggcggatgaa
    41941 aaaaagttcc cgaagttccc ttgactcttg aaaagcggag gagtaaactt tccctaccta
    42001 ccgttcggta ggaaaggaga aatatgactc gagacgatgt tcttgatgca gccgcacagg
    42061 tttttcggca aaagggcttt catggggcct ccatgtctga tatcgcatct gccttggatg
    42121 tgcaaaaggc cagtttgtat catcatgtga cctcgaagca ggagatattg ctggcgcttt
    42181 tggaacgtgc cctgggaatg cttacagaac atattgcctc gttcgctgcc cgaaccattc
    42241 ctgccgatca aaaattacgc ctgatgatcc gcgggtatct ttctgccctc gccgaaaatg
    42301 ccgacctgac gtcggttttg ctttttgagc atcgttctct cgataaaaag tctcatactc
    42361 gccatgttcc acaaagggat cggttcgaag ggttgtggca tgatgtgctc gatgaaggag
    42421 tacgtgaaaa ggtttttgat ttgaaagata cggggctggc tgtccgcgcc attatgggta
    42481 cattaaattg gacgctcacc tggtatcgtc ccgatggtcc caaatccatc gagcaaattg
    42541 ctgatgagta ttctgacttt attttgaagg gtttgttgaa atagttcacg gattgtcata
    42601 agccgaagga aaaggagtaa gcaaatgtat tcgttcgaac ccaatgaaga acaacaaatg
    42661 ttgatcgacg cggtggggaa gttcgcttcc aacgacctgc gtccagccgc acatgatgcg
    42721 gaagagagtc atgaaatgcc acagaagctc atcaacaaag gctgggagct tggcctgctg
    42781 caagcatcca cgcctgaagc gtatggtggc tttggtgaac gcagtgctgt gaccaccgtc
    42841 ctcgctgtgg aagaaatggc cttcggcgat ctcgctggcg c
//
LOCUS       DAOQLC010000019        40860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_266_length_40860_cov_26.340277, whole genome shotgun sequence.
ACCESSION   DAOQLC010000019 DAOQLC010000000
VERSION     DAOQLC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 40860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40860
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_40860_cov_26.340277"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PL000_05205"
     CDS             <1..492
                     /locus_tag="PL000_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNC88323.1"
                     /translation="RVLTSLFVDRGVKIDRTYQLNFGGNTDFLNMLERERLESKKISK
                     TNAVTSMIPYEIDPSNVHVGPSDHVPWLTDRKWCYIRMEGTTFGNVPLNMELKLEVWD
                     SPNSAGVMIDAVRCAKIALDRELAGPIVGPSSYFFKTPPKQFKDEIAHQKVEDFIAGR
                     SDE"
     gene            548..1702
                     /locus_tag="PL000_05210"
     CDS             548..1702
                     /locus_tag="PL000_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC88324.1"
                     /translation="MIKKRLFPTLILLAILLAGCQPAAPTADSVAIQTAAFETALAQI
                     SIPTQTPAPTDTAIPTATVPRTPPALPSIYQSPILKPEDLPRTYVADTCTYLQSKWNP
                     NNAAPGTVVMPIMIHAIDQDANSSQLLKISGKDFRQMMNDLHEFGFQAINMQQMADFM
                     YNNARIPQRSVLLIVDDRAYAEKFNTHFRGYFEDWGWPVVNAYIGLDERPDLWAENAA
                     LSAEGWVDYQSHGYIHNVPITDSSTDEYITAELSGAITSIQKYMNKTPIAYIWPGGGF
                     SRRAVELAPQLGYQLGFTVNPRGPVMYNWVPQADAFNASNPYAIPEVPAGNPLMTLPR
                     YADINARSELDNVRQISEQAAAYAEQNKATELEYYDIMCAPTYGPIPPAQ"
     gene            1929..3032
                     /locus_tag="PL000_05215"
     CDS             1929..3032
                     /locus_tag="PL000_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88325.1"
                     /translation="MKPFPESLRTILYRLTTLLIIVFILISCEPFTTSQQIEAQSISS
                     TDTALAQANHTQAPPRTPPALPAVFQSPYLNPLDTPRTYIQNPCQYLHNKWNPLSADP
                     GTVVMIVMFNDILMEDFDRIMEQLHEQGFEAINTKELLAFMERNVKIPQRSVVIIQND
                     SQQADDLTRNYREYYDRWKWVVVNGWTSNPDAPEYLWQDNIALENEGWVDHQAQGMIP
                     GTVLSDESSKTVIQRELENPITAFIKLYNKNPIAIIWPGGGFGMRPVEAARQLGYQLG
                     FTANTRGPVMYNWVPLADEVDPERPTYIPEGQINDPLMTLPRYWPEQIMDAIDEVRLT
                     GNEASAYAKANKKAELEYYDIVCADTYGPMPTP"
     gene            3057..3401
                     /locus_tag="PL000_05220"
     CDS             3057..3401
                     /locus_tag="PL000_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNC88326.1"
                     /translation="MSDCIFCKIIKGEIPSTGVYRDEQVTAFRDINPAAPTHVLIVPN
                     KHIDSVNMMSAEDEPLLGHLFMAAKTIAAQEGVAEGGYRLVVNTNANAGQTVFHIHLH
                     LIGGAPLKLQMG"
     gene            3586..>4304
                     /locus_tag="PL000_05225"
     CDS             3586..>4304
                     /locus_tag="PL000_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNC88327.1"
                     /translation="MPEQEIYLLSPRALSAETIAVAFAKTSRAPESFREIAAELSDEK
                     SAQFHEKWVVGYGHASVAEHAVLHIAFENVSRVAIETIESSRLASYTEKSTRYQKWGP
                     DDFTIPPELQEVGHPLRDEFIQTVRLLFSTYAESLEPVKSLVVERTPRRDNESDEAYD
                     RRIRSQYVDRCRFILPAAANANVGMTANARIIEMVIRKMLSHPLAEVRQIGEKVKEVS
                     KAETPTLVKYADAVQYVVETV"
     assembly_gap    4305..4404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4405..5058
                     /locus_tag="PL000_05230"
     CDS             <4405..5058
                     /locus_tag="PL000_05230"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNC88328.1"
                     /translation="DWCALIDYDKDGEKKILAAALYRFNEMPYKEALAYVKTLKKKER
                     EGLAEALLGKLGKYDVPLRELEYATYTFDLVMDQGAYAEFKRHRMMTQTPQRLTTRLG
                     FTIPLLVTEAGFGSRYEAAMQSASDMYEKLHAFNPDVAQYIVPNGFNRRVLAQFNLRE
                     AFAFCQLRSAANAHFSIRRVAQRIYEEMTRVHPLLTKYMKLREETWQQVEENYFTQV"
     gene            complement(5130..6113)
                     /locus_tag="PL000_05235"
     CDS             complement(5130..6113)
                     /locus_tag="PL000_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC88329.1"
                     /translation="MKYRHLGKTGIQVSELSFGSWVTFSNQADVKAAVDMMAAAYDAG
                     VNFFDNAEVYASGKSEEVMGAALKQLNWRRGSYLVSTKLFWGLHDGINEKNTLNRKRL
                     IEGIRGSLQRLQLEYVDLIYCHRPDKTTPIEETVWAMHNIIEWGHALYWGTSEWAASE
                     IVEAIQIAERHHLHKPVVEQPQYNMFVRNRLEGDYERFYKEYNYGTTIWSPLASGLLT
                     GKYKGGIPKDSRGALKGYDWLQNHLTDKQKLAKVDALEPIAKELGCSLSQLALAWCAK
                     NPFVSTVITGASRVDQVHENMKAMEVIEKITPEIMARIDEIFEIKKDEDDD"
     gene            6186..7094
                     /locus_tag="PL000_05240"
     CDS             6186..7094
                     /locus_tag="PL000_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC88330.1"
                     /translation="MQKTLILCAMLLSACASSNGQPSDGPLQPYLTRTPVNTSTPNTI
                     VIINTPIPTATPFTYIIQQGDTFSELAEKFKVTQDDLQAANPDVSPNSMSVGTTLLIP
                     GSFLTAADASTPTPVPAPVTQSICHPAADGGLWCFAMIQNNSAGFLENVSAQITLDPS
                     NTATAFTPLDIIPPNSSMPVYVFFPNTPAALGANPQVQVLSAIQLSANPPRYLKATLN
                     NAIAQIDWDGRSAQLSGEILLPAESTAATQVWVAAVAYDQLGQVVGISRWEGEAIQAG
                     GSLKFNFAVSSLRSTIDAVDFFIEAR"
     gene            complement(7153..8778)
                     /locus_tag="PL000_05245"
     CDS             complement(7153..8778)
                     /locus_tag="PL000_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC88331.1"
                     /translation="MANPFSLKFRHGSFAIRTLLLVLVCSLILSASARDVFAFQVDIP
                     FDQALVYSGGESTNLRDYDPATTYSSGDKLVFSGLVSFDPHLNLTPDLAETWDVSDDG
                     TVYTFHIRANAKFHSGRFVTAQDFIYSWERAVSPELASDTALTYLGDIVGVREMNTGQ
                     AEHISGLAAIDERTLQVTIDAPKPYFLLKLTYPTAFVVDQSNVESGEEWVRDPNGTGP
                     FRLAEWVSNEYKVYAANEDFYLGMPSIPYVIVKLYAGDDVRLFETGDVDVAGVGLYSV
                     DRMLDPEEPLHNQLVTGVNLCTGYVVFDTTQPPFDDVNVRKAFSMAFDRQRYIDVVLR
                     GRALPAFGPFPPGLPGFNYQLKGLPYDPQQARELLKQSKYGGPEGLPPIVYTNGGIGS
                     YISSSVAATVEMWEQNLGVNLTVENIEYNFYNQQIYSGNHGQIFGGGWCADYPDPENF
                     ADVLFHSGSKQNNSGYSNPALDALLEQARIEQDVTKRIAMYQQAEQMIVDDAPVLFTT
                     HSLSYELVQPYVKGYVFTPIAVPLERYLWIDGK"
     gene            complement(8771..10135)
                     /locus_tag="PL000_05250"
     CDS             complement(8771..10135)
                     /locus_tag="PL000_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNC88332.1"
                     /translation="MHLRKILSVLLLLAVVPVQSVFAAAGAEVQDDAVVFDFPNTATF
                     SATLKSAAAITAVTLEYGNEQQTCGEVIAKAFPQFDPSTTVQVEWTWDMRQSGSLPPG
                     STIWWRWRYTDESGAEFVSDQKTAIWLDDVHNWQTLSSDELRVHWYDNDKAFAQTMLD
                     AGTEGLRRNREQAGLSIEDVVDIYVYPNYDDMRDAILYEPSWTGGMAFSDHNIVIMGI
                     SASDSNWDKNTVIHELTHVLVGHFTFSCIGTVPTWLNEGLAMFSEGELDSYMQSQLDD
                     AIKNDTLLTIRSLNGGFSELPDKANLSYSQSHSIVEFLISTYGQDKMTEILAALRDAK
                     PIDDALIEVYGFDTDGLEDAWREAIGAAPRTVSAQPTAQPTPTFVPTYVPVSGAPLAV
                     TPTPYSVPTSSFVNTTPPIAKGPPIALTIALICMCGVILLILGVMVIGILARSQNQKA
                     GKNG"
     gene            10434..10916
                     /locus_tag="PL000_05255"
     CDS             10434..10916
                     /locus_tag="PL000_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNC88333.1"
                     /translation="MQKNKFILGGLLILAAVVYLIFSSTQASAEYFMTVDEIHAEGDT
                     AIGKSLRLSGAVLGDTIQYDADTLTLTFEVAHVSGDNAEIEAQGGLAVVLKDAVNDPS
                     RQRITVVYVGPKPDLLRGEAQAIMTGTLGDDGIFYADELLLKCPTKYEEAVPEQAATN
                     "
     gene            11000..13066
                     /locus_tag="PL000_05260"
     CDS             11000..13066
                     /locus_tag="PL000_05260"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HNC88334.1"
                     /translation="MFVEFGYGVLLVSFLVAIYSIFAAVYGVSNKSTAMVESARRAML
                     LTFPLLSISAAVLVYLLVNNHFEVSFVYEVTSRSMPTYLKVTAWWGGQAGSLLFWSWL
                     LSAFASAVTIRKWERDIEFLPWVIVICSTTLVFFVGMVAFYENPFTRFWLVGGNVEPH
                     LFAPLNATLFTADDGNGLNPLLRHPGMVIHPPMLYLGFVSFVIPYAFAMAALITGRTD
                     DRWIRLTRRWSLWAWLFLSFGLVLGGRWAYDVLGWGGYWGWDPVEIAAFMPWLTGTAF
                     LHSVMIQEKRGLLKHWNMILIILTYALVVFGTFLTRSGVLSSVHAFANSPIGPLFMGF
                     VSISLIVSIALVIWRWPELRSETEMKSMLSREALFLLNNLLFMSVLVVCFWGVIFPLI
                     SELFTGQKVTVGPPFYERATAPLFAALMFLMGVAPLSAWGHSTVTTLGKAMWKSAVAA
                     VVVTGILLATYTQNVIALVGFFLIAFVIFVTLQEFWRAARARQRAQNENFFTSLTRLM
                     GRNRRRYGGYIIHISMMLMAIGILGIEIFQTETQGSLAKDEQLSISGYTLQYRELASW
                     DDRGEGVNYTRAVVDVYKDGEYIGELNPRIDYYFDSQQNMTIPGNRSTLKDDLYVLLV
                     DWQPVSNTGATFKIFVNPLVNWLWIGSLVFMLGVIFAAWPDRDPAEEPVRSGAARTAR
                     QTSAAD"
     gene            13138..13632
                     /locus_tag="PL000_05265"
     CDS             13138..13632
                     /locus_tag="PL000_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNC88335.1"
                     /translation="MKKLFYSFTFAVLMAALFTGISFAQDTTPPTDDEVNAVARQLYC
                     PVCESTPLDVCPTEACKDWREQIRVMLSEGKSEEEILKYFEAQYGARVLAEPPKKGFY
                     WLIYLLPPAIILLGAVILLRSMKQWTTKPVAESTGMDEAGDASSAVKDDYVARLEEEL
                     KKQK"
     gene            13681..14250
                     /locus_tag="PL000_05270"
     CDS             13681..14250
                     /locus_tag="PL000_05270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC88336.1"
                     /translation="MTEETTNTKRGVPVWVQVVIWAFLVGLLGLVGFGLNRARNPMAE
                     IGKPVPDFDIVYYEGYEYDSQPQMKLSDLQGKIVLINIWASWCKPCEQEAPELEEAWQ
                     FYKNSNDVVFIGVDYVDTPAGANEYLTKFNITFPNAPDLQSTISSILNRQMGVPETYI
                     IDREGVLRHIKIGPFASVDEIKSIIQGIE"
     gene            14263..14775
                     /locus_tag="PL000_05275"
     CDS             14263..14775
                     /locus_tag="PL000_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC88337.1"
                     /translation="MELGSIFLVLAVIVVVFAYLYAPFTARARRLRNGETHQVSSLKA
                     ERDRVINSLQELDFDFNLGKIPEGEYPEQRAALLQKGADILRKLDELAPVSSSALNAE
                     ARIEKATAAGRADSAAVSAVLDSDDDIEAMIAERRKSHKSKSAGFCPKCGKPVLSADK
                     FCPSCGKALK"
     gene            14817..16679
                     /locus_tag="PL000_05280"
     CDS             14817..16679
                     /locus_tag="PL000_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC88338.1"
                     /translation="MKLRHIVFITAIAILLTACNFTLAADVTPPPGYASPTPMPTLGP
                     LYPASNPDIHNGAVIYMEKCAPCHGNAGLGDGPQGKELPVTVAAIGLPATATKAKPSD
                     WYTQVTQGNLERFMPPFSSLSDQERWNVVYYAFTLHILPEQGDLGQTLFEENCADCEK
                     YFSDLKTMSALSEDEIVQIIKNGSGEIPAFGKDFSDDEALAVAAYLRTVIFAPPPAQP
                     TPVPASETPLSAEAGTPSAVETPNGTEQAQTTPEADNSFLTTFGNISGTVDNQTGAAL
                     PSNLTVKLRAFEHGGDPSAGPEEVDSTEVTINADGSYQFENMEIPESRIFVAELELEG
                     ISYQTDFAIVEAGMTSLVLPPLTVYATTEDLSTLKINEAQMYFDFANTEDVQIFTVYS
                     FTNTGNKTILVNMGADQVVPFIAFPKDAKALGYEAMQNSAAFVPTDGGFAMPPSELPY
                     GLIAFASVPKAREVAISQSAVLDIESISLYLPQGMEAEGATLSDAGLQNMETMSFQVY
                     SATGLKKGESIEFTVSGEPSATSETADLTQNQTLLIGIGAFGLVLIIAGGWLYIRDRK
                     KEEDLDDEDGEFEDTESTMDAIIALDDLHREGKISDEAYQKRRAELKSALKKQK"
     gene            16684..17463
                     /gene="ccmA"
                     /locus_tag="PL000_05285"
     CDS             16684..17463
                     /gene="ccmA"
                     /locus_tag="PL000_05285"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HNC88339.1"
                     /translation="MKRLKVASGRHQIPPATFNLPPSNPTHMIEVKKLVKRFGLKVIL
                     RGLDFQVQPGEFVALLGPNGAGKTTFLRILASLSRPSLGEVRVAGYKLPHEAAQVRAR
                     LGVVSHLPLLYPDLTAEENLRFYARMYGIEDQEARITEVLGMVGLEPRRRDLVGAFSR
                     GMQQRLAIGRAVLHDPEVMLFDEPYTGLDQDASEMLDEVLRSVAAKGRTVVMTSHDLA
                     RAEELATRFDILSRGVIATSAKREDLKSTNLLAFYKQSLES"
     gene            17463..18149
                     /locus_tag="PL000_05290"
     CDS             17463..18149
                     /locus_tag="PL000_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNC88340.1"
                     /translation="MKRSSPSLLKATLAIVQKDLAAEFRSRELLSAMLVFSLLVILIF
                     NFALELDVKVRQSVTAGVLWTTFAFAGTLGLNRSMAVEKDRGCIDGLLLAPVDRSAIY
                     FGKAISNLAFMLIVEAIVLPVYSMLYNINLFQLDLLGVILLGSIGYTAVGTLLSAMAV
                     QTRTRDVLLPILLFPIAVPVLLAAVKASGGMLAGAEFAEILVPLNILIVYDIVFIAVA
                     FMVFDFVVEE"
     gene            18229..18924
                     /gene="ccsA"
                     /locus_tag="PL000_05295"
     CDS             18229..18924
                     /gene="ccsA"
                     /locus_tag="PL000_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC88341.1"
                     /translation="MQPKPTALKVLDIASIIVLAISTYLALFFAPTELVMGDVQRVFY
                     FHIGTAWVGLLGFIIAAGSGIIYLITKDLKWDRFEVAAVEVSTVFFFLTIVLGSIWAR
                     PAWNTWWTWDPRLTTAAVTELIYVAYFMLRQGIDDPEKRARFGAVYTLLGGISAPVTF
                     MVIRLFRSIHPVVIGAANANQEKMSMTPDMRVAFFFALFAFTVIFIDLIWHRLRLGSM
                     EEKVEQLKLKVSM"
     gene            18939..19109
                     /locus_tag="PL000_05300"
     CDS             18939..19109
                     /locus_tag="PL000_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88342.1"
                     /translation="MESTPDTSSYMIAGYVISFITMGIYVASLYIRNNNILRDIETLE
                     SMQEEKPKATKK"
     gene            19161..20807
                     /locus_tag="PL000_05305"
     CDS             19161..20807
                     /locus_tag="PL000_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88343.1"
                     /translation="MNMTRRWMLIFLALTVACSGAVFTRDISEALGNGFQPVSANNPP
                     PGPDRYTYITVQYTAYEWYLATWKDQVPLCSLITDHEGMPLPGEVYRDCGETIYEKWI
                     VQKPCMATNKRSCTGFYISPVKNYPAEKEVPAELEPATVWVSLEGCETVLSTSTNFCE
                     VAPTLVFTGQEPLPGETIIRVEGTYDGAPFKCEGTNVCKFEIPSTDEDGAKIEFWAFS
                     SYGDSSLIYSAQIRVQKVDEGDPDQLYWYADVLSTQWTGEAVATCSGAWGVFPPVGGP
                     PTWLTTPKYSEELSSDIPYTYLAANLIRQGVVDASSCADGGLTPEGGVNQCGLEAARP
                     AVTQWQNQFDELILTTAQQTAVPARLLKNLFARESQFWPGSFQGMDDTGLGQLTENGA
                     DTTLFWNSSFFEQFCPFVLSEETCGNGYVNIKEEERAELRRALVGSVNATCENCPLRI
                     DLPQANYSVGVFAHSMIANCEQTGQVIKNYTGQNAGEVASYEDLWKFTLVNYNAGGGC
                     LAEALTYTLGSSLPLTWENVSPFLAGACSGAVDYVNDISK"
     assembly_gap    20904..21003
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21016..22242)
                     /locus_tag="PL000_05310"
     CDS             complement(21016..22242)
                     /locus_tag="PL000_05310"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNC88344.1"
                     /translation="MPAPRISRRQFLQGCSAAIASMAGARLTNLAFAKQDNPSDTLVV
                     VFLRGGWDALNVVPPLQGDDRGFYEKARPDIKISNLLSLNDQFGLHPALAPLHELYQA
                     GKMGVVHAVGLNHDTRSHFDAQEYIELGTPGSKNTTSGWITRHLQETGVSSILPVVST
                     TGQPTSLLNFVPTVNVSTPEDFSQWGNDLIGSQQTALRQMYQGETLLHRAGLRTLDAL
                     DIVSPLVSQEYQPANGAFYNDDEFGQQLKTIARMIKLDAGMRVAAVDFGGWDTHEYET
                     DGNGGYIGDLLGQLASGLSNFYLDLDSGYTDKLSVVVISEFGRRLVQNESYGTDHGHG
                     SVMLALGGNVNGGRVYGQWPGLNNDQLYDHADLAITTDYRQVLSELLSTRLGNSNLEG
                     VFPGFSPSDNPLGIFR"
     gene            complement(22249..23979)
                     /locus_tag="PL000_05315"
     CDS             complement(22249..23979)
                     /locus_tag="PL000_05315"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HNC88345.1"
                     /translation="MNSKQFSRRDFLKLGGSLATASLLAACQPGEETSTAPLSPTTSA
                     NDISIPEPAVSLPPVSLAILALGRMTFGIRPGDIEAFNALGSTDDERLRAFVQQQLNP
                     DSLDDSDFESRYSAAGFQALHKSHEQLYSDHIANNAYDANDDAYWEWYSLPAYELVDA
                     TLLRAVFSKKQLVELLADFWHNHFNIFFWQDDGVPLLVSYNRDVLRRHMLGNFRQMLE
                     AVASHPSMLYYLNQNNSSDAGPNENFARELFELHTLGAENYLGVRDPNTVEKDANGIA
                     IGYVDNDVYEAARCLTGWRIDDDLGEWEEGVGITGNFIYYKPWHDRFNKLVLGKYIPA
                     DQPDMQDGKDVLDLLAAHPGTARHIARKLCRRFIADLPPDSVVEKVAAAFTEFRDAPD
                     QLKRVMEVLLLSDEFKQSWGQKIKRPLEACISTLRSLNSDFTRVPGGVPWMLSLMGQP
                     LFERRSPDGYPDVKEAWANSMSLLYRWNFAVGVAENWLNDDDKGLTLHTDLLNQTPAE
                     IRTAESLADFWIQRILGRPLSSDADRNSVIAVMAQEYGTQEPLPEDHVAYVLPAMVEV
                     ILMSPDFQWK"
     gene            complement(24002..24709)
                     /gene="lipB"
                     /locus_tag="PL000_05320"
     CDS             complement(24002..24709)
                     /gene="lipB"
                     /locus_tag="PL000_05320"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNC88346.1"
                     /translation="MFPFTIEDLGLIEYEKAWKLQDDYAAEIAEGKRPPTLLLLEHPH
                     VYTFGRKGHAENLLWNEEQLKQKGIATHWVDRGGDVTYHGPGQLVGYPLLSLGKLNNE
                     NKLPEADYVGYVRKLEETLITALMHFGIVSGQRSGLTGVWIQSDVHSRCPRCSPEDRK
                     KPAKIAAIGVKVDARGITRHGFALNVEPDMEYWNGIIACGLNEPVVSLADLLDPAPSM
                     QQVKAEVTRAFREVFAL"
     gene            complement(24765..26150)
                     /locus_tag="PL000_05325"
     CDS             complement(24765..26150)
                     /locus_tag="PL000_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC88347.1"
                     /translation="MNNTFTQLENFGHSLKAPSYLIKPQKSEEIYEAFRSAKQMGLTV
                     AARGGGRSYNDAALNGGGIVLDTSGMNQILEWNPATGQVRCEPGVTLKQLWQKVLPDG
                     WWPPVVSGTMFTTLGGCLSANIHGKNNFRMGTIGEHVLEFTAVLPTGAEVTCSPNKNG
                     DLFHSMISGLGMLGIFTSVTMQMKKMHSGLLEVHAWPSHTLKDHLASLEDGAPNYDYI
                     VGWLDTINTIGHGQIHAANYLHEGEDPEAQKTMQLSYQHLPDRLFGVMPKSMLHYFMT
                     PFANNLGWGLVNTAKYISSLRKHTFRQSHAAFHFLLDYVPLWELSYGKGGLIQYQSFL
                     PKETAHQAWTEMLALAKKRGLPSYLGVTKRHRPDKFLLTHAVDGFSLALDFKVTNRTR
                     VRLSAMLQDFDKIVLNAGGRFYFAKNSETSAETARAFYGEETVEKFRKLKKRCDPNGL
                     LESDLYRRIFS"
     gene            complement(26197..26985)
                     /locus_tag="PL000_05330"
     CDS             complement(26197..26985)
                     /locus_tag="PL000_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC88348.1"
                     /translation="MSNSPKPAQATPLNPRRRGIIVGASDGMGAALARKLAKEGYTLA
                     LIARRQDKLASLCAEINAAEGEARALAYAHDVSNYDEVPNLLRKITAELGGLDLLVFM
                     AGVNFPPGLNSYNFEGDRKMIEVNLIGAMAWLSPVAEMFQSAKAGQIVGISSVAGDRG
                     RVGNPGYNTSKAALTTYLEALRNRLTRHGVNVLTVKPGFVKTEMLKAAQGGTPFAISP
                     EQAAEDIYKAMRKRKQLIYTASIWRWIMLAIQHVPSVIFRRLSF"
     gene            27209..28480
                     /locus_tag="PL000_05335"
     CDS             27209..28480
                     /locus_tag="PL000_05335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNC88349.1"
                     /translation="MAGITEKQKNIFHHAREIIKQDESIPEEEKEGIFKLIRVLVEVA
                     DLAPSKLPNQEESPTAFTQEIISRHALMAAVKQQIDELESLKKLSLNVTSSLDLQTVL
                     DTVVAEAMRLVKNSRAAHIFLYSHGKLEFGSSLTAEGLKNMPVAMPRKEGLTATVVAS
                     GVPIIVEDITDHPLYTNVSQNWRGSIIGLPLKYNKSIVGVMNLSRSTVGGFTRAELRL
                     IGLVADLAAVAISNASLHQQVAAQANTDSVTGLPNRRALDERIQEEIRFARRMNSEFS
                     VVMMDLDGFKAVNDTYGHVVGDELLYSLFNYLAENIRPTDFLARYGGDELTLVSREAN
                     QDMAESVTQKIIDLVRGYKPAFAEKKNIQLGITAGIAIYPTHSKTAGDLLRAADAALY
                     QAKKHHRGSYSIAKITTGPLDPITLPRRKPE"
     gene            28575..29498
                     /locus_tag="PL000_05340"
     CDS             28575..29498
                     /locus_tag="PL000_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNC88350.1"
                     /translation="MPRKVKLILNPMADMGRAWKTANDLRPIAQEFKGELTWSGTVYP
                     THAIELARQAAEEGYDLVIAMGGDGTAHEVMNGLMQVPAEKRPVMGVVPIGSGNDFAY
                     SMGITQKSAHALAHMLKAETIQSIDIGQMTDEHNRKEYFDNTLGIGFDAVVTIRSHKL
                     PIVKGFLMYLTAVIQTIILNHNPAHIKIETESEQWETSTLMVTLCNGPREGGGFMLSP
                     NSKNDDGKMEYVIVNKVSRGMMFRLVPEFMNGTHMRFKQIRMGEFKKCSITSDRPLYI
                     HADGEIFTSFGSNLRKVNFEILPQALKVVRG"
     gene            29552..31168
                     /locus_tag="PL000_05345"
     CDS             29552..31168
                     /locus_tag="PL000_05345"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNC88351.1"
                     /translation="MPSLYQTFLKHDLGHLRIVADLWGLELESQDLDSAAEELSASLL
                     DLEAVTETIDILSPESRAALDAIVESNGKMEWAAFTRAHGEIRGMGAAKRDRERPHLK
                     PISPAEHLFYRGILARAFFESSKGSQEFAYIPDDLLEVIEMLVVEDDEAPTPAPHGEP
                     LGRPATPVEKAVELPATDHILDDATTYLAALRIGSRAAEGVSTRRRELHELLEAAHLI
                     KDDALQPEAVKNFLGASRAEALNILYKAWFVSNTFDELRLLPGILCEGEWSNQPQVTR
                     EFLTNLINDIPQNKWWSLPALLRDIKTKYPDFQRPAGDYDSWFIKRESDGQYLRGFAY
                     WDQVDGALIKYFVQTLHWLGRVNLAAPEEGKESTAFFLLSSFVERNEEKGKIVVSSNG
                     KITISRYFSRAVRYQIARFCEWDEEKGNEYFYRVTAGSLKHANEQGLKSEQLLALLVK
                     YTNGNVSPALVKALKRWDTSGVEARVESLLLLRVSRPEIMEEIRKSKAGKYLGEVLSP
                     TAVIVKSGAIQKVMAELAELGLLAEVTTHE"
     gene            31161..31400
                     /locus_tag="PL000_05350"
     CDS             31161..31400
                     /locus_tag="PL000_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88352.1"
                     /translation="MNRGRRIILIVAVILALIPVPMTCGHMFYGCATGPDMSGNYYTY
                     YEVEPLGITLIETLIMTNLKIYYWSGKEAHFIGIP"
     gene            31466..32644
                     /locus_tag="PL000_05355"
     CDS             31466..32644
                     /locus_tag="PL000_05355"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNC88353.1"
                     /translation="MSKTDWIQQEIDNLKSQGLYNNIRTIGSPQGAWLTVDGKKVLNF
                     CSNNYLGLANHPTLTAAAKKATDEMGVGPAAVRSIAGTMTLHVELEKRLAQFKGVEAA
                     ITFQSGFTANLATVPALVGKEDVIFSDRLNHASIIDGCRLSGAKIIAYEHNDVKSLEE
                     QIKANLSQYRRALIITDGVFSMDGDIAPLPDIYEVAKKYDILLMVDDAHGEGVLGKGG
                     RGIVDHFGLHGKVDVEVGTMSKAFGVVGGIVAGKSVIIEWLRQRGRPFLFSSAVTVPD
                     AAACLAAVDLLEDSTQLVDKLWDNAKYFKAEMKNLGFNTGVSETPITPIMLGEAPLAQ
                     QFSRELFEAGVFAMAIGFPTVPQGKARIRVMISAAHAKDDLDKGLEAFKTVGKKLGVI
                     "
     gene            32770..33393
                     /locus_tag="PL000_05360"
     CDS             32770..33393
                     /locus_tag="PL000_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88354.1"
                     /translation="MNVPIFPDNINLSRPFHFTTENADSKARIGGNMPEELSISLNEN
                     AQYFGTFPLYDNESPLYFSLFINCSFKEFVVDWHGRIKDDSKVVVALHEYRPRSKSMQ
                     YASRLKSQHSIEILKTREDIYQSDTGELVVREGHKFGGAPYCIQEPKLIGWEELQAQG
                     YRQILQIDFPGLEDGAVSGTWPFGDGIFNLFWKYPFEGEKYFWCVQS"
     gene            33554..33853
                     /locus_tag="PL000_05365"
     CDS             33554..33853
                     /locus_tag="PL000_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNC88355.1"
                     /translation="MSTDPTFRMTIQDVFSIKGRGTVLTGRIESGTITVGDEIIIKGR
                     NSSKKAIVAGVEVRRKVTTSARSGDEAGIRLKDTEEDVQQGDILTGSEHDFTWKP"
     gene            34038..34790
                     /locus_tag="PL000_05370"
     CDS             34038..34790
                     /locus_tag="PL000_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC88356.1"
                     /translation="MSGHSKWSTIKRKKGVADAKRGAVFTRLAREVTIAAREGGDPDT
                     NFRLRLAVDKARAENMPKDNIERAIKKGTGEDKEGTTFEELTLEGYGPHGSALMVTCV
                     TDNRNRTVPDMRHAFSKSGGNMAEPGAVAWQFDRKSYFAFPASKLSFDKAFELGIEAG
                     ADDVVDGGDTIEIYAPVESFKTIADALHKVHVQPEEASLRMIPKQEIELDVESTLKFM
                     KMVETIEDLDDVQDVFHNVKVSDEALAALEAA"
     gene            34839..35321
                     /gene="ruvC"
                     /locus_tag="PL000_05375"
     CDS             34839..35321
                     /gene="ruvC"
                     /locus_tag="PL000_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC88357.1"
                     /translation="MTLALGIDPGTATTGYGLVRLMPNGELVTVSYGIISTPKDATPP
                     ARLEMLFDDLNKLLKKHKPETAAVEKLFFSKNVTTALAVGQARGVIMLCLQKAGIEPF
                     EYTPNEVKQAVAGYGSADKRQVQEMVRALLQLDSIPKPDDAADALAIAITHLNTKRYE
                     "
     gene            35403..35978
                     /gene="ruvA"
                     /locus_tag="PL000_05380"
     CDS             35403..35978
                     /gene="ruvA"
                     /locus_tag="PL000_05380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNC88358.1"
                     /translation="MIATLRGEITQIEENAIVVEVGGVGMRVFVPAPLRTNAKAGEIL
                     FMFTHLVVREDALTLYGFESQADRDLFNILLGVDGVGPKVALSVLSTLTIDAIQRAIF
                     ADEADLLNSVPGVGKKTAQKMALHLKDKLKPTDALGKIAAMADYDSEVLAALTALGYS
                     VVEAQAAIQSLPKDAPKEVEERLRTALGYFQ"
     gene            36124..36972
                     /gene="proC"
                     /locus_tag="PL000_05385"
     CDS             36124..36972
                     /gene="proC"
                     /locus_tag="PL000_05385"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNC88359.1"
                     /translation="MLKKKIAFIGPGVMAEAMIAGLLRKKLADAKNITASGPREERGV
                     ELNKKYGIKATTDNAAAIHEADVVVLSVKPQRLTEVMKGLKGIRSDALVVSIIAGATM
                     KKISSGLKHKTIVRSMPNTPGQIGEGITVWTASKEVTDEQQEMTRAILGALGEEVFVE
                     DESYLDMATALSGSGPAYVFLFTEALIDAGVHMGFPRRIAEQLVLQTIKGSASYYQGA
                     SRHPATLRNQVTSPGGTSAEALYYLEKAGFRTAISRAVWAAYQRSLELGKEKPGHIDT
                     HDEESA"
     gene            37037..38299
                     /locus_tag="PL000_05390"
     CDS             37037..38299
                     /locus_tag="PL000_05390"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC88360.1"
                     /translation="MKKTITLLTSGTRGDVLPYIALGEGLLDAGYQIRIAAPLGFANL
                     VQQSKLTFAPFEGNPSDLLIEQGDSTPMTLGTNPIRSIRATQKFIGQARPLYRRMLHT
                     AAEACRGSDMLIHGLPTIWGAHIAEGLGIPAVRAVLQPLAPTREFPSALLPFRSSFLG
                     IGNWLTHWAVTQATWLSWRREINHARHSDFGLGAAHWLDPSLRADSNQPLTLNGFSTQ
                     IVPRPKDWNDKQVVTGYWRKNELTNNHVDRPDLQHFIDSSPNPIAIGFGSPGTRGYLP
                     MLEEALQHANAQAILTIPAKWHGEIKSKNIFPIEYAPHDWLYRRVKVAVHHGGAGTTS
                     ASLHAGIPTVTLPLAIDQFFWGEQIHKIGIGPRPIPQRKLNAENLSQAITQALTDHAM
                     NAKAKTVSAELNKENGIQAAVSQIRKLL"
     gene            complement(38316..39503)
                     /locus_tag="PL000_05395"
     CDS             complement(38316..39503)
                     /locus_tag="PL000_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88361.1"
                     /translation="MNSSLVFIFITIFVDLLGYGIMLPLLPFYVQAQDGGAAIAGALS
                     SLYAGTQLFAGPILGALSDRFGRRPVLLVCLFGTSLGYFMLGLADTLPLIFLAVFIDG
                     LTGANLTTAYAYIADVTTSENRARGMGMVGAAFGLGMMAGPALGGLLSKYGLYVPAFT
                     ASAIALGNVIFGYFVLPESLPLEKREARSVSEMFNWMKHFRGLFNQANIQKLLVTMFL
                     LNLAFAGLQSNFPLYSNFRFHWTPTQNSFFYLYVGVCAVFIQGFLFGKLQPRFGEKRL
                     ILFGLSLMVIGLAGMALAPQAWMLYPAVTVVALGTGTSIPSLTALVSLRVSESEQGRL
                     MGGTQTLLSLTNIFGPMLAGVSFEVIAFSAPYWVGSIFSLTALGVAYMSLRGNASHQT
                     VLD"
     gene            complement(39588..>40860)
                     /locus_tag="PL000_05400"
     CDS             complement(39588..>40860)
                     /locus_tag="PL000_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC88362.1"
                     /translation="MTTKSDVQQHDVLIIGGGPVGLSCAYYLLKSGRKVTILDSKEIG
                     KGSGSGNAGHVVPSHIIPLAAPGVVTSALKWMLDPAHSPFGMKISLDPKYLMWLLRFV
                     LACSESNVQRGIPPLNSLGQLSAKNFAQMIAEEKFDCHYQEKGLLFLYKTEEAFSGGR
                     HEGDLMQKHGIPVSVYDKAKVHEIEPAALDDVIGGVHFTGDSHMNPALFLKMLSDRVR
                     DMGAEMLENTPVTGFESAQGKVRVVRTAVGDAKHEAEHVILAAGALTPSMARDLKLNL
                     PIQPARGYSLTMSATKQMPRHALILGERRVAVSPMGDLLRFTGRLEVGNGSMTPDPRW
                     IQRIENSAREYLRIDETLDIKETWAGLRPTTPDGVPIIGRSPNHSNLVLATGHAMLGL
                     SLGPGTGQVVAELVNGKETAFDLSPLRLERF"
BASE COUNT        10195 a        11230 c        10128 g         9107 t
ORIGIN      
        1 cgtgtgctga ccagcctctt cgtagatcga ggcgtgaaga tcgaccgcac ctatcaactc
       61 aatttcggcg gcaataccga cttcctcaac atgttggagc gcgaacgtct tgaaagcaaa
      121 aaaatctcca agaccaatgc agtgaccagc atgatcccct acgagatcga ccccagcaat
      181 gtacacgtcg gccccagtga ccatgtaccc tggctgaccg accgcaaatg gtgttacatc
      241 cgcatggaag gcaccacctt cggcaatgtg ccgctcaaca tggaattgaa gctcgaagtg
      301 tgggacagtc ccaactcagc aggtgtcatg atcgacgcgg tacgctgtgc caagatcgcc
      361 cttgaccgcg aactggctgg ccccattgtt ggcccatctt catacttctt caaaacaccg
      421 cccaagcaat tcaaggatga gatcgcccat caaaaggtgg aagacttcat tgcaggcaga
      481 agcgacgaat aaataaagta gggacacggc ggcgccgtgt ccctacttta ttggtagaat
      541 tcggcgcatg ataaaaaaac gactctttcc taccctcatc ctgcttgcca ttctactggc
      601 aggatgccaa cccgcagccc ccaccgctga ctctgttgcg atccaaaccg ccgcattcga
      661 aaccgcattg gcacagatca gcatccccac ccaaactccc gcccccaccg acacagccat
      721 ccccactgcc actgttccgc gtacaccacc tgccctgccc tccatttatc aatcacccat
      781 ccttaaacca gaagacctcc cgcgcaccta cgtggcagac acctgcacct acctgcaaag
      841 caaatggaat ccgaacaatg ccgcgcccgg cacagtggtt atgccgatca tgatccacgc
      901 cattgatcag gatgcaaact cctcacaatt attgaaaata tcgggaaaag atttcaggca
      961 gatgatgaac gacctgcatg aatttgggtt tcaagccatc aacatgcagc aaatggccga
     1021 tttcatgtac aacaatgcca ggatccccca gcgctccgtc ctgctgatcg tggatgaccg
     1081 tgcctacgcc gaaaaattca acactcactt ccgcggatat ttcgaagact ggggctggcc
     1141 cgtcgtcaat gcctacatcg gtctcgatga acgtcctgac ctgtgggctg aaaatgcggc
     1201 gctctctgcc gaaggctggg tggattacca atcgcatggc tacattcaca atgtccccat
     1261 caccgattcc tccaccgacg aatacatcac cgccgaatta agcggggcga tcacctccat
     1321 tcaaaaatac atgaacaaaa cacccatcgc ctatatctgg ccgggcggcg gcttctcgcg
     1381 ccgcgccgtg gaattggctc cgcaattggg ctatcaattg ggattcaccg tcaacccgcg
     1441 tggaccggtc atgtataact gggtgccgca ggcggatgct tttaatgcct ccaatcccta
     1501 cgccattcct gaagtacctg ctggcaaccc gctgatgact ttgccgcgtt acgcagatat
     1561 caacgcccgc agcgagttgg acaacgtccg ccagatcagc gagcaggctg ctgcctacgc
     1621 agaacaaaac aaggccaccg aactcgaata ctacgacatc atgtgcgcgc cgacctacgg
     1681 gcccattccg cctgcacaat aaaacgaacc gtgatgttaa tgtacgctga ctctgccgat
     1741 cgctaaccga atcagtttcc ccgtcggtac gatacgccct gtccgaattt cgtttttggc
     1801 tcaagccacc gtcctcggcg gcgttctgcg aataaaccct tcgccgggga cggcgaaggg
     1861 agcaactcaa ccagtttccc cgtcggtacg atacgccctg tccgaatttc gtttacaata
     1921 aagaagacat gaaaccattt cctgagtctc tcagaaccat actttaccgc ctaaccaccc
     1981 tcctcatcat cgtttttatt ctgatttcct gcgagccttt caccacttcc cagcagatcg
     2041 aagcccaaag catcagctcc actgatacag cccttgcgca agcaaatcac acccaggcac
     2101 caccccgtac accgcccgca ctgcccgcgg tgtttcaatc tccatacttg aatccgctgg
     2161 atacgccgcg cacatacatt caaaatccat gccagtatct gcacaacaag tggaatccgc
     2221 tcagcgcaga ccctggtacg gtggttatga tcgtgatgtt caacgatatc ctgatggaag
     2281 atttcgacag gatcatggaa caacttcacg agcagggatt tgaggccatc aacacaaaag
     2341 aattgctggc attcatggaa cgaaacgtca aaatccccca gcgttcggtg gtcatcattc
     2401 aaaatgacag ccagcaggca gacgacctga cccgcaacta ccgcgaatat tatgaccgtt
     2461 ggaaatgggt ggtggtcaac ggctggacca gcaacccaga cgcacccgaa tatttatggc
     2521 aggataacat cgcactggaa aatgaaggct gggtggacca tcaggctcaa ggcatgattc
     2581 caggcacagt gctttcagat gaatcttcca agacagtgat ccagcgcgaa cttgaaaacc
     2641 cgatcactgc gtttatcaag ttgtacaaca aaaatcccat cgcgatcatc tggcctggcg
     2701 gcggattcgg catgcgcccc gtagaagcgg cacgccagct tgggtatcaa cttggcttca
     2761 ccgccaacac ccgcggacct gtgatgtata actgggttcc cctggccgat gaagttgacc
     2821 ctgaacgccc cacctacatt cctgaaggcc agatcaacga tccattgatg accctgccgc
     2881 gctactggcc cgaacagatc atggatgcca ttgacgaagt ccgcctgact ggcaacgaag
     2941 ccagcgcgta cgcaaaagcc aacaaaaaag cggaactcga atactacgat atcgtttgtg
     3001 ccgataccta cggcccaatg ccaaccccgt aaccaaccat ctttcggagg tctcccatgt
     3061 ctgactgtat tttttgtaag atcatcaaag gcgaaattcc aagcacgggt gtgtaccgcg
     3121 acgagcaagt gactgccttc cgcgatatca accctgcggc gccaactcat gtgttgatcg
     3181 tgcccaacaa acacattgac tcagtcaaca tgatgagtgc cgaagacgag cccttgctgg
     3241 gtcacctgtt tatggcagcc aaaaccatcg ccgcacaaga aggcgttgcc gaaggcgggt
     3301 atcgtctggt ggtcaacacg aacgccaacg caggtcagac cgtttttcac atccacctgc
     3361 acttaatcgg tggagcgcct ctgaaactcc aaatgggata gggttctgtt taagataaaa
     3421 tacccagcga tcagcaaagc ctgctggtcg cttttttata acccaggcca ttgaccgagg
     3481 acttaaattc gtccatttga cctcttacat aagttgcaag gaatgtcttt gaaatgcctt
     3541 tttgaaccac gaagacactg aatcacggag tttttaggag acccaatgcc cgaacaagaa
     3601 atttatctgc tttccccgcg cgcgctcagc gcagagacca tcgccgtggc gtttgcaaaa
     3661 acctcacgcg cgcccgaatc ctttcgtgag atcgccgcag aactaagcga cgaaaaatca
     3721 gcacagttcc atgagaaatg ggtggtcggg tatggtcatg cctcggtcgc cgaacatgcg
     3781 gtcttgcaca tcgcatttga aaatgtttcg cgtgtggcca tcgaaaccat cgaaagcagc
     3841 cgcctcgcct catacaccga aaaatccacg cgctaccaga aatggggacc tgatgacttc
     3901 accattccgc ctgaactgca ggaagtgggg cacccattgc gcgacgaatt catccaaacc
     3961 gtgcgcctcc ttttctcgac ctatgctgag tcattggagc ctgtgaagag tctcgtagtg
     4021 gaaaggaccc cgcgccgcga caatgaaagc gacgaagcct acgaccgccg catccgctcg
     4081 caatatgtag atcgctgccg attcattctg ccagcagccg ccaacgccaa cgtaggcatg
     4141 accgccaacg cccgcatcat agaaatggtc atccgcaaga tgctctcaca tccgcttgcc
     4201 gaagtgcgcc agatcggcga aaaggtaaaa gaggtgtcaa aagccgaaac acccaccctg
     4261 gtgaaatatg ccgacgcggt tcagtatgtg gtggagacgg taaannnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnngattgg tgcgctttga tcgactacga caaggacggc
     4441 gaaaagaaaa ttctcgcagc ggcactgtat cgcttcaatg aaatgcccta taaagaagcc
     4501 ctggcttacg tcaaaacatt gaagaaaaaa gaacgtgagg gcctcgccga agcattgctt
     4561 ggaaaactcg gaaaatacga cgtccccctg cgcgaactgg aatatgccac ctacaccttt
     4621 gatctggtca tggatcaggg cgcgtacgcc gagttcaaac gccatcgcat gatgacgcaa
     4681 accccacaac ggctgacaac ccgactcggg tttacgattc cgctgctcgt aacagaagca
     4741 ggtttcgggt ccaggtatga ggcggcaatg caatctgcaa gcgacatgta tgagaagctg
     4801 catgcgttca atccagatgt cgcacagtac atcgtcccca atggattcaa ccgccgcgtg
     4861 ctggcacaat tcaacctgcg tgaagcgttt gcgttttgtc aattgcgcag tgcggcgaat
     4921 gcgcatttct cgatccgccg tgtggcacaa aggatttacg aggaaatgac gcgtgtccac
     4981 ccgttgttga cgaagtacat gaagctgcgc gaagagacct ggcagcaggt tgaagaaaat
     5041 tacttcacac aggtataaac gcaggggtac agcaatgctg tgcccctgaa aaatcaaaag
     5101 gcggagcatc tcatgctccg cctttgtttt taatcgtcgt cttcgtcttt cttgatctca
     5161 aagatctcat cgatccttgc catgatctcg ggggtgatct tttcgatcac ttccatcgcc
     5221 ttcatgttct cgtgaacctg atccacacgg cttgcccctg taatgaccgt actcacgaac
     5281 ggattcttcg cgcaccatgc cagcgcaagc tgagaaagtg aacagccaag ctctttcgca
     5341 atcggctcca gggcatccac ttttgcgagc ttttgcttat cggtcaaatg gttttgaagc
     5401 caatcatagc ctttgagcgc accacggctg tctttgggaa tgccgccttt gtatttcccc
     5461 gtcaacaacc cggaagccag cggactccaa atggtggtgc catagttgta ttccttgtaa
     5521 aaacgctcgt agtctccttc aaggcggtta cgcacaaaca tgttgtactg cggctgctcc
     5581 accacaggct tatgcaagtg atgacgttcc gcgatctgga tcgcttccac gatctcagag
     5641 gcagcccatt ctgaagtacc ccaatacagg gcgtggcccc actcaataat attgtgcatg
     5701 gcccaaacgg tttcttcgat gggtgtggtc ttgtcggggc ggtgacagta gatcaggtct
     5761 acatattcga gttgtaatct ttgcagtgaa ccgcgaatcc cttcaatgag gcgcttacgg
     5821 ttgagcgtat tcttttcgtt tatgccatca tgcaggcccc agaaaagttt tgtagaaaca
     5881 aggtaactgc ccctgcgcca gttcaattgc ttaagcgccg cgcccatcac ctcttccgat
     5941 ttaccgctgg catacacttc agcgttatca aaaaagttca cacctgcatc gtaggctgca
     6001 gccatcatat ccacagcggc tttcacatcc gcctgattgc taaatgtaac ccacgaacca
     6061 aacgaaagct cacttacctg aatacctgtc ttgccaagat gacgatactt catgatgtct
     6121 ccattgattt tagaaactcg caacgctcca acattgcggc acattccgtc cattataatc
     6181 atcccatgca aaaaacactg attctttgcg ctatgcttct cagcgcatgt gcttcttcaa
     6241 atggtcaacc atcagacggg ccgctgcaac cataccttac ccgcaccccc gttaatacat
     6301 ccacgccaaa cacgatcgtc atcatcaaca cgcccatccc aaccgcaacc ccattcacct
     6361 acatcattca acaaggcgac accttcagcg aactggctga aaaattcaaa gttacacaag
     6421 acgatctgca agctgcaaat cctgatgtct ctccgaacag catgagcgtc gggacaacgc
     6481 tcctcatccc gggcagtttc ctcaccgcag cagacgcctc caccccgacc cctgtgcctg
     6541 ctcctgtcac ccaaagcatt tgccaccctg ccgcagatgg cggcctgtgg tgttttgcca
     6601 tgatccagaa caactctgcg ggcttccttg aaaatgtctc tgcccagatc accctcgacc
     6661 caagcaacac cgccaccgcc ttcaccccat tggatatcat cccgccgaat tcctccatgc
     6721 ctgtgtatgt cttttttcca aatacacctg ctgccttggg ggcaaatccg caggtgcagg
     6781 tgttgagcgc cattcaattg agcgcaaatc cgccgcgcta tctcaaagcg accttgaaca
     6841 acgccatcgc ccaaattgac tgggatggcc gcagtgcgca actcagcgga gagatccttt
     6901 tgcccgcgga atcaacagcc gccacacagg tttgggtggc ggctgttgcg tacgatcagt
     6961 tgggtcaggt ggtgggaatc tcccgctggg aaggcgaagc gattcaggct ggggggagtc
     7021 taaaattcaa tttcgccgta tccagcctca ggtccacgat tgacgcggtg gattttttta
     7081 tcgaggcaag gtagatgaac tttctttcct ctttcttctt tcgtcatgca aagaaagagg
     7141 aaagaagcaa aattacttcc catcaatcca taaatatctt tcaagcggaa cggcaatggg
     7201 cgtaaacaca tagcccttta cgtatggctg caccaactca taggaaagcg aatgggtggt
     7261 gaacaacacg ggggcatcat ccacgatcat ttgctcggct tgctggtaca tggcgatgcg
     7321 tttggtcaca tcctgttcga tgcgtgcctg ttcgagcagg gcatccaacg cggggtttga
     7381 atagccgctg ttattttgct tcgacccgct gtggaataaa acatcggcaa aattttcggg
     7441 gtcgggataa tcagcgcacc acccgccgcc aaagatctga ccatgattcc cggaatagat
     7501 ctgctggttg taaaagttgt attcgatatt ctccacggtg agattcacac caaggttttg
     7561 ctcccacatt tccactgtgg cggccacgct ggaactgatg tagctgccaa tgccgccgtt
     7621 ggtatacaca atgggaggca aaccttccgg gccgccgtat ttggattgtt tgagtaattc
     7681 acgtgcctgc tgggggtcat agggcaggcc tttcaattga taattgaatc caggcaggcc
     7741 aggcgggaaa gggccgaagg cgggcaaagc ccgtccgcgc aaaacgacat caatataacg
     7801 ctggcggtcg aatgccattg aaaatgcctt gcgcacattg acatcgtcaa aaggcggctg
     7861 tgtcgtgtca aagaccacat agcctgtgca aagattgaca cccgtcacca gttgattgtg
     7921 gagcggttct tcaggatcga gcatacggtc cacgctgtag aggcccacac ctgccacatc
     7981 cacatcgcct gtttcaaaga gacgcacatc gtcgcctgca tacaatttga cgatcacata
     8041 gggaatggac ggcatgccga ggtaaaaatc ttcattcgcc gcatacactt tgtattcgtt
     8101 ggacacccat tcagcaaggc ggaacgggcc tgtgccgttc gggtcgcgca cccattcttc
     8161 gccgctttcc acattggact ggtccacaac aaaggcagtg gggtaggtaa gtttcaaaag
     8221 gaaatatggc ttgggcgcat caatggtcac ttgcagtgtg cgttcatcaa tggcggccaa
     8281 cccgctgata tgctctgcct gacctgtgtt catttcacgc acgccgacaa tatcgccaag
     8341 ataggtcaat gccgtgtctg aagccagttc agggctgaca gcccgttccc aggaatagat
     8401 gaaatcctgg gcagtgacga aacgtccgct gtggaatttg gcgtttgcgc gaatgtgaaa
     8461 tgtgtatacc gtgccatcgt cgctgacatc ccatgtttcg gcgaggtcag gtgtgaggtt
     8521 gagatgcggg tcaaaagaaa ccaatccact gaagacgagt ttatcgcctg agctgtatgt
     8581 ggtggcgggg tcgtaatcgc gcaggttggt tgattcgccg ccgctgtaca ccagggcttg
     8641 atcgaaggga atatccactt gaaatgcgaa cacatccctt gcgctggcgg agaggatcag
     8701 agagcagacg agaaccagca agagagtgcg aatcgcaaac gaaccatgcc tgaatttgag
     8761 tgaaaatgga ttagccattt ttacctgcct tttgattttg agaacgagca aggatgccga
     8821 taaccattac acccaagatg agcagaatca caccgcacat gcaaatcagg gcaatggtca
     8881 atgcaatggg cgggcctttg gctatgggtg gagtagtatt gacgaaagaa gaggtgggaa
     8941 cagaataggg tgtgggcgta accgccagag gcgctcctga aacgggcaca taagtgggga
     9001 caaaggttgg ggttggctgt gctgttggtt gggcagaaac agttcgaggc gcagccccga
     9061 tcgcttctct ccaggcgtct tccaatccat cagtgtcgaa tccgtacact tcgatgagcg
     9121 catcgtcaat cggcttggca tcgcgcaagg ccgccaggat ctcggtcatc ttgtcctgcc
     9181 cataggtgct gataagaaat tcgacgatgc tatgcgactg actgtaagaa aggtttgcct
     9241 tgtcgggaag ttctgagaag ccgccgttca acgaacggat ggtgagcagg gtgtcatttt
     9301 tgatggcgtc atccaactgc gattgcatgt aagaatccag ctcgccttcg ctgaacatgg
     9361 ccagaccttc gttcaaccat gtgggcactg ttccaatgca agagaaagtg aaatgaccga
     9421 cgagcacatg cgtcagttca tggatcactg tgttcttatc ccaatttgaa tcagaagcgg
     9481 agattcccat gatgacgatg ttgtgatctg agaacgccat gccgccagtc catgatggtt
     9541 cgtagagaat ggcatcacgc atgtcatcgt agttggggta gacataaata tccaccacat
     9601 cttcaatgga caggcctgcc tgctccctgt tgcggcgcag gccttccgta cctgcatcaa
     9661 gcatggtttg ggcaaaggct ttgtcgttgt cataccagtg tacgcgcagt tcgtcactgg
     9721 aaagggtctg ccagttatga acgtcatcga gccagatggc ggtcttttgg tcactgacaa
     9781 attcggcacc agattcatct gtgtatcgcc agcgccacca gatcgtggaa ccaggcggca
     9841 gcgaaccgct ctgacgcata tcccaggtcc actccacttg caccgtggtg gatgggtcaa
     9901 actgtggaaa cgccttcgcg atcacttcac cacaggtttg ctgttcattt ccgtattcca
     9961 atgtgaccgc tgtgatggcg gcggccgatt tgagtgtggc tgaaaatgtg gcggtattcg
    10021 gaaaatcaaa aacaaccgca tcatcctgaa cctcggcacc tgccgctgca aaaacagact
    10081 gcaccggtac gacagccagc agcaataaaa ccgacagaat ttttcttaag tgcatctttc
    10141 caactccttg gtttggggca attttcttct ttcaatgtct gcccaaaata taacattaac
    10201 cgcttttgcc ctgcgagggt accctggtca aaggtcaatc atcaaggcaa aacaaaatat
    10261 cacatccaaa taagacaaat tcgggaaaga ttcatgacat ccatcagccc tcaaaactaa
    10321 gggtcgttta atgtcatcat gtaaaatgga tgtagattgt gatattcata attattcaac
    10381 tcaaaaaagc ctgatcggtc tttaccgaaa aagcgattcg ttcggaggaa tccatgcaaa
    10441 aaaacaaatt cattctcggg ggcttgctca tacttgcggc agtggtatat ctgatcttct
    10501 catccaccca ggcgagcgcc gaatacttca tgaccgtgga cgaaatccac gcagaaggcg
    10561 ataccgccat tggcaagagc ctccgccttt caggcgcagt gctcggggat acgatccaat
    10621 acgatgcaga tacgctcacc ctcacctttg aggtggcgca cgtttccggt gacaacgccg
    10681 aaatcgaagc gcagggcggg ctggctgtcg ttttgaagga tgcggtcaat gacccaagcc
    10741 ggcagcgcat cacagtggtt tatgtaggcc ccaaacctga cctgctccgc ggtgaagcac
    10801 aagccattat gactggtacg ctcggagatg acggcatctt ctatgcagac gaattgctcc
    10861 tcaaatgccc caccaaatat gaggaagcgg ttccagaaca ggccgcaacg aactaaaaga
    10921 acaacctgac gggtggatgc ctgtcaggtt ttgtttctaa cacaaacttt cactgcacac
    10981 aaaaaacaaa ggaaacctta tgttcgtaga attcggatat ggcgttttat tggtaagttt
    11041 cctcgtcgca atttatagta tttttgcggc ggtatatggc gtttccaaca aatccaccgc
    11101 catggtggaa agtgcaaggc gcgccatgct gctcaccttt cccctgctct ccatttcagc
    11161 cgctgtgctg gtctatttgc tggtcaataa tcacttcgaa gtctccttcg tttatgaagt
    11221 gaccagccgc agtatgccaa cctacctcaa ggttacagcc tggtggggcg gacaggccgg
    11281 ttcgctcctc ttctggtcgt ggctgctttc agcgttcgct tctgcggtca ccatccgcaa
    11341 gtgggaacgt gatatcgaat ttctgccgtg ggtgatcgtg atctgctcca ccacgctggt
    11401 ctttttcgtt gggatggtgg ccttctatga gaatccattc acccgtttct ggctggtcgg
    11461 tggaaatgtg gagccgcacc tctttgcccc gctaaatgcg actctcttca ccgctgacga
    11521 tggaaacggc ctgaacccgc ttcttcgcca cccgggcatg gtcattcatc cgcccatgct
    11581 gtatcttggt ttcgtatcat tcgtcatccc ctacgccttt gccatggccg ccctcatcac
    11641 cggccgcacc gatgaccgtt ggattcgcct cacccgccgc tggtctttgt gggcctggct
    11701 cttcctgtcc tttggcctcg tactcggtgg acgctgggct tacgacgttt tgggctgggg
    11761 cggctactgg ggctgggacc cggttgagat cgcagccttc atgccgtggt tgacgggtac
    11821 cgccttcctg cattctgtga tgatccagga aaaacgcggc ctgctcaagc actggaatat
    11881 gattctcatc attctcactt atgccttggt ggtgtttggc accttcctca cacgctctgg
    11941 cgtactttcc tcggtccatg cctttgccaa cagccccatt ggcccgttgt ttatggggtt
    12001 tgtttccatc tcgttgatcg tatcgatcgc gctggtgatc tggcgctggc ctgaattacg
    12061 cagtgaaaca gagatgaagt caatgctttc acgcgaagct ttgttcctgc tcaataactt
    12121 gttgttcatg agcgtgttgg tggtctgttt ttggggtgtg atcttcccgt tgatctccga
    12181 actgtttaca ggtcagaaag tgaccgttgg ccctccattc tatgaacgtg ccaccgcccc
    12241 gctcttcgcc gctttgatgt tcctgatggg cgttgctccg ctttctgcct ggggacattc
    12301 cacggtgacc acccttggca aggcgatgtg gaaatctgcg gtcgctgccg tggtggtgac
    12361 gggtatcctg cttgccactt acacacaaaa tgtcatcgca ctggtgggtt tcttcctcat
    12421 tgcgtttgtc atcttcgtca ctttacagga gttttggcgc gccgcccgtg ccaggcagcg
    12481 ggcacagaat gaaaacttct tcacttcact caccaggctg atgggccgca atcgccgccg
    12541 ctatggtggt tacatcatcc acatcagcat gatgttgatg gccattggca tccttggcat
    12601 tgagatcttc cagaccgaaa cacagggttc ccttgcaaaa gatgaacaac tgagcatttc
    12661 cggatacacc ctgcaatacc gtgaactggc ttcatgggat gaccgtggcg aaggcgtaaa
    12721 ttacacccgc gccgtggtgg atgtgtacaa agatggtgaa tacatcggcg agttgaaccc
    12781 gcgtattgac tattacttcg actcacaaca aaacatgacc atccccggca accgttccac
    12841 acttaaggat gatttatacg tcctgctggt ggattggcag cctgtttcca acacgggagc
    12901 cacctttaag atattcgtga atccgctggt gaattggttg tggatcggca gcctggtctt
    12961 catgcttggc gttattttcg ccgcctggcc cgaccgtgac cctgccgaag aacccgtgcg
    13021 aagcggagcg gcacgcacag cccgccaaac cagcgcagca gattgatcgc aagttaggtt
    13081 tcaggttaca agttacaggt tgcaagaaaa atcaacctga aatttccagg agcatcgatg
    13141 aaaaagcttt tttattcttt caccttcgca gttttaatgg cggccctgtt taccggcatt
    13201 tcatttgcgc aggataccac cccgcccacc gatgatgaag tgaatgcggt tgcccgccag
    13261 ttgtattgcc ctgtttgcga aagcaccccg ttggatgttt gtccgaccga agcttgcaaa
    13321 gattggcgtg aacagatccg tgtgatgctc tcggaaggca aatccgaaga ggaaatcctc
    13381 aagtatttcg aagcacaata tggcgctcgt gtactggctg aacccccgaa aaaagggttc
    13441 tactggctga tctatttgct gccgcccgcc atcatcctgc tgggtgccgt catcctgctg
    13501 cgctcaatga aacagtggac gaccaagccc gttgctgagt caacaggcat ggacgaggca
    13561 ggagacgctt cgtcggctgt gaaagatgat tatgtggcac gtctcgaaga agaattgaag
    13621 aaacaaaaat aaattcctct catctacggc cttgccggga taaggatcat ggagaatgtt
    13681 atgaccgaag aaacaacgaa cacaaagcgc ggcgtaccag tttgggtgca agtcgtcatt
    13741 tgggcttttc tggtgggcct gttggggctg gtgggctttg gcctgaaccg tgcccgcaac
    13801 cccatggctg agatcggcaa gcccgtgccc gatttcgaca ttgtgtatta tgaaggctac
    13861 gaatacgaca gccagccgca gatgaaactc tctgacctgc aaggcaagat cgtcctcatc
    13921 aacatttggg cttcgtggtg caaaccctgc gaacaggaag cgcccgagtt ggaagaggca
    13981 tggcaattct acaaaaacag caatgacgtg gtcttcatcg gcgtggatta tgtggatact
    14041 cccgccgggg ccaacgaata cctcaccaag ttcaacatca ccttccccaa tgcgcctgac
    14101 ctgcaatcca ccatttcaag catactcaac cgccagatgg gcgtacccga aacatacatc
    14161 attgaccgcg aaggcgtttt gcgccacatc aagatcggcc cctttgcttc agtagatgaa
    14221 atcaagtcca tcattcaagg catcgaatag ggggaatgac acatggaact aggctcaatc
    14281 tttctggtac tcgctgtcat cgtggtggtc tttgcctatc tgtatgcgcc attcacagcc
    14341 cgtgcgcgcc gacttcgcaa cggagagact catcaggtct cctcgctcaa agccgaacgt
    14401 gaccgtgtca tcaattcact gcaggaactc gatttcgatt tcaacctcgg caagatcccc
    14461 gaaggggaat accccgaaca acgtgcagca ctcctgcaaa aaggcgcaga cattctccgc
    14521 aaactcgatg aactggcacc tgtctcctcc tctgcactca acgccgaagc acgcatcgaa
    14581 aaagccacgg ctgcaggccg cgccgattct gccgcagtat ccgccgtttt ggatagcgac
    14641 gacgatatcg aagccatgat cgctgaacgc cgaaaaagcc acaaaagcaa atcggctggc
    14701 ttttgcccga aatgcggcaa acccgtttta tccgcagata aattctgccc ttcctgcggc
    14761 aaagctttga aataactggt gatcggcaag caatcaccaa cccatcgagg aacttaatga
    14821 aactacgtca tatcgttttt attactgcaa tcgcgatctt actgaccgcc tgtaacttca
    14881 cactcgcagc cgatgtcacc ccgccgcccg gttatgcctc ccccaccccc atgcccaccc
    14941 tgggtccgct ataccctgcc agcaatcctg atattcacaa cggcgcggtc atctacatgg
    15001 aaaaatgcgc accgtgtcac gggaatgcag gtttgggcga cggcccgcaa ggcaaggaat
    15061 tgcccgttac agtagccgcc atcggcctgc ccgccacagc tacaaaagct aaaccctccg
    15121 actggtacac ccaggttaca caaggcaacc tcgaacgctt catgccgccc ttctccagcc
    15181 tgagcgatca ggaacgctgg aacgtggtct actacgcgtt cacgcttcac attctgcccg
    15241 agcagggcga tctcggccag accctcttcg aagagaactg tgccgattgc gaaaaatatt
    15301 tcagcgacct caagacgatg tcggcacttt ctgaagacga aattgtgcag atcatcaaaa
    15361 atggatcggg cgaaatcccc gcctttggaa aagatttcag cgacgacgaa gcccttgccg
    15421 ttgcagccta cctgcgcact gtgatcttcg ccccgcctcc cgctcaaccg acacctgttc
    15481 ccgcctccga aacgcctctc agcgccgaag cagggactcc gtccgctgtg gaaacgccca
    15541 atggcaccga gcaggcccaa accacgccag aagcggataa ttcattcctc accacgtttg
    15601 ggaatatcag cggcaccgtt gataaccaaa ccggggcagc gttgccatcc aacctcacgg
    15661 tgaaattgcg cgccttcgag cacggcggcg accccagcgc cgggccagag gaagtggatt
    15721 caaccgaggt caccatcaac gccgatgggt cctatcaatt cgaaaatatg gaaattccag
    15781 aaagccgcat cttcgtagcc gagttggagc ttgaaggcat cagctatcaa accgatttcg
    15841 ccattgtgga agctggaatg accagtttgg ttctgccgcc gctcaccgtg tatgcaacca
    15901 cggaagatct ttccacattg aagatcaacg aagcgcagat gtactttgat tttgccaaca
    15961 ccgaagacgt acagatcttc acggtttatt cgttcaccaa cactggcaac aagaccatcc
    16021 tcgtcaacat gggcgccgat caggtcgtgc cgttcatcgc cttccccaaa gatgccaaag
    16081 cccttggcta tgaagccatg caaaattccg ccgcttttgt acccactgat ggcggttttg
    16141 ccatgccccc cagcgagcta ccctacggcc tgatcgcatt tgcctctgtg cccaaggcca
    16201 gggaagtcgc catttcacaa tctgccgtgt tggatatcga gagcatttcc ctctacctgc
    16261 cgcaaggcat ggaagccgaa ggcgcaaccc tcagcgatgc tggcttgcaa aacatggaga
    16321 ccatgagttt ccaggtctat tctgcaacgg ggctcaagaa gggtgaaagc atcgaattca
    16381 ctgtcagtgg agaaccctcc gctacatctg aaactgccga cctcacccaa aaccaaaccc
    16441 tgttgatcgg aatcggcgca ttcggtcttg tcctcatcat tgcaggcggg tggctgtaca
    16501 tccgtgaccg caagaaggaa gaggatcttg acgatgaaga tggcgaattc gaagacaccg
    16561 aatccaccat ggacgccatc atcgccctcg acgacctgca ccgcgaaggc aagatctcgg
    16621 atgaagccta ccaaaaacga cgcgctgaat tgaaaagcgc attgaagaaa caaaaataaa
    16681 aaaatgaaac ggttgaaagt tgcaagtgga aggcatcaaa ttccacctgc aactttcaac
    16741 ctgccacctt caaaccctac tcacatgatc gaagtaaaaa aactcgtcaa acgctttggg
    16801 ctaaaagtca ttctgcgcgg actcgatttt caagtgcagc ctggcgaatt tgtggcattg
    16861 cttggcccca atggtgccgg caaaaccacc tttttgagaa tcctcgcctc actttcccgc
    16921 ccttcgctgg gtgaagtgag agtggcaggc tacaaacttc cgcatgaggc cgctcaagtg
    16981 cgtgcgcgcc tgggtgtggt ctcacatttg ccgctgctct acccagacct caccgcagaa
    17041 gaaaacctga gattttacgc gcggatgtac ggcattgaag atcaggaagc ccgcatcacc
    17101 gaagtgttgg gcatggtggg gcttgagccg cgccgcagag accttgtcgg tgccttctca
    17161 cgcggcatgc agcaacggct cgccatcgga cgcgcggtct tgcatgaccc tgaagtgatg
    17221 ctgttcgacg aaccctacac cggcctcgat caggatgcct ctgaaatgct cgatgaggta
    17281 ctgcgttcgg tcgctgcgaa aggccgcaca gtggtgatga cctcacacga tctcgcccgc
    17341 gccgaagaac tggccacccg cttcgatatc ctttcgcgtg gagtgatcgc cacctcagcc
    17401 aaacgggaag acctcaaatc caccaacctg ctggcttttt acaaacaatc actggaatca
    17461 tgatgaaacg ttcatcccca tccctcctca aagccacgct ggccatcgta caaaaagacc
    17521 ttgccgcaga attccgctcg cgtgagttgc tctccgccat gctggtcttt tcgcttctgg
    17581 tcatccttat tttcaacttt gccctggagt tggatgtcaa agtacggcaa tccgtcaccg
    17641 ccggtgtgct gtggaccaca ttcgcttttg caggcacgct tggccttaac cgttccatgg
    17701 ctgtggaaaa agatcgcggc tgtattgatg gcctgttgct cgccccggta gaccgctccg
    17761 ccatttattt tggcaaggcc atcagcaacc tcgccttcat gttgattgtc gaagccatcg
    17821 tgctgcctgt gtacagcatg ctatacaaca tcaacctctt tcaactggat ctgctcggcg
    17881 tgatcctgct gggctccatt gggtacacgg cggtcggcac cctgctttca gccatggcgg
    17941 tacaaacccg cacccgtgat gtgcttctac ccatcctgtt attccccatc gctgtaccgg
    18001 tgctgctggc agcggtgaaa gcgagcggtg gaatgctggc cggagccgag tttgcagaga
    18061 tcctcgttcc gctgaacatt ctgatcgtat atgacatcgt cttcatcgcg gtggcgttca
    18121 tggtgttcga tttcgttgta gaagagtgaa cgagacagca aatcagagat tcagcgagcc
    18181 aaccatggat tggcattcgt aaatcgcaaa ttttagcaag gagttcatat gcaaccaaaa
    18241 ccaactgctc tcaaagtact cgatattgca tccattattg tgctggcaat atccacctat
    18301 cttgcgcttt tcttcgcccc cactgaactg gtgatgggag atgtacagcg tgtcttctat
    18361 ttccacattg gcacagcctg ggtcggtctg ctgggcttca ttatcgcagc aggatcaggc
    18421 atcatctacc tcatcaccaa agacctgaaa tgggaccgtt tcgaagtggc cgctgtggaa
    18481 gtgagcacgg tcttcttctt cctgacaatt gttcttggtt ccatttgggc ccgccccgcc
    18541 tggaacacct ggtggacttg ggatccgcgt ctgaccactg ccgccgtcac tgagctgatt
    18601 tatgtagcct actttatgct ccgtcagggc attgacgatc ctgaaaaacg cgcacgtttc
    18661 ggcgcggtat acaccctgtt gggcggaatc agtgccccgg tcaccttcat ggtcattcgc
    18721 ctgttccgct ccatccaccc tgttgtcatc ggcgctgcca atgcaaatca ggaaaaaatg
    18781 agcatgaccc ccgatatgcg tgtggccttc ttctttgccc tgtttgcctt taccgtcatc
    18841 tttattgacc ttatctggca ccgccttcgc cttggaagca tggaagaaaa ggtcgaacaa
    18901 ctcaagctca aagtttcaat gtaaacggag aaaccacaat ggaatccaca cccgatactt
    18961 ccagctatat gatcgcaggc tacgtgatct ccttcatcac catgggaatc tacgtcgcca
    19021 gcctgtatat ccgcaataac aacattctgc gggatatcga aaccctcgaa tccatgcaag
    19081 aagaaaagcc aaaagcaacg aaaaagtaac agccaggaat gacagagtcc gcttataata
    19141 taggcggact ctgttttttg atgaatatga cgcgccgctg gatgctcatt ttccttgcct
    19201 tgaccgttgc ctgttcaggc gcggttttta cgcgcgatat ttccgaagcg ctcggcaatg
    19261 gcttccagcc tgtttctgcc aataatcccc cgcccggccc tgaccgctat acctatatta
    19321 ccgtccaata cactgcctac gaatggtatt tggcaacctg gaaagatcaa gttcccctct
    19381 gttcactgat caccgaccat gaagggatgc ccctgccagg cgaggtgtac cgggactgcg
    19441 gtgaaaccat ctacgaaaag tggattgtgc agaagccatg catggcgacc aataaaagat
    19501 cctgcaccgg cttctatatt tccccggtaa agaattaccc tgcagaaaaa gaagttcctg
    19561 cagaacttga acctgccacg gtttgggtct cgttggaagg ctgtgaaact gtattaagta
    19621 catcgacgaa tttttgcgaa gtggcaccca cgttggtctt tacgggtcag gaacccctgc
    19681 ccggcgaaac catcatccgc gtagaaggga catacgatgg ggcgcctttc aaatgcgagg
    19741 ggacaaacgt atgtaaattc gaaatcccct ctacagatga ggatggagcg aaaatcgaat
    19801 tttgggcctt ttccagttat ggagatagca gcctcattta cagtgcccag atccgagtcc
    19861 aaaaagtgga tgagggtgac cctgatcaat tgtattggta tgccgatgtg ctttccacac
    19921 aatggacagg tgaagcagtg gcaacctgct ccggagcctg gggggtcttc ccacccgttg
    19981 gcggaccgcc cacctggttg accacaccca aatatagcga agaactcagc agtgatattc
    20041 cctacaccta tcttgccgcc aacctgattc gtcagggggt tgtggatgcc agttcctgtg
    20101 cagatggtgg tctcacgccc gaaggtggag tcaaccaatg cgggttggaa gcagcccgcc
    20161 ctgcagtgac acaatggcag aaccagttcg atgagttgat cctcactacg gcgcagcaaa
    20221 cagctgtccc tgcgcgattg ctgaaaaatc ttttcgcaag agaaagccag ttttggccgg
    20281 gcagttttca gggtatggat gatacgggtc ttggtcaatt gacagaaaat ggcgcagata
    20341 ccaccctgtt ctggaatagt tccttcttcg agcaattttg tcccttcgtt ttatcagaag
    20401 aaacctgcgg caatggttat gtcaacatca aggaagagga acgggcggaa ttacgccgcg
    20461 cactggttgg cagtgtgaac gcgacctgcg agaattgtcc attgcgcatt gacctgccac
    20521 aggcaaatta ttcggtgggc gtcttcgctc attcgatgat cgccaactgc gagcagacgg
    20581 gacaagtcat caaaaattac acaggccaga atgccggtga agtggcctct tatgaagacc
    20641 tatggaagtt tacgttggtc aactacaatg caggcggggg ctgtctggca gaagcgctta
    20701 cttacaccct tggctcaagc ctgccgctta cctgggaaaa tgtatcgccg ttccttgcag
    20761 gggcttgttc aggcgcagtg gattatgtaa acgacattag caagtaaaaa aacagatata
    20821 aaacaccccg aaattaatct ttcaattctt tggccgcgaa tttacacaaa aaagccgatc
    20881 cattttacag ggtcggcttt tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnntctttgc ttaaactatc gaaatattcc cagcgggttg tcacttggag aaaaccccgg
    21061 gaagacacct tcgagatttg aatttcccaa acgtgtagat agcaactcac tcaaaacctg
    21121 acggtaatcg gtggtgatcg caagatcggc atggtcgtac agttgatcgt tattcaaccc
    21181 gggccactga ccatacaccc gcccgccgtt gacgtttccg ccaagcgcca gcattaccga
    21241 gccgtgaccg tgatcggtgc cataggattc gttttgcaca agccgccgcc caaactcgct
    21301 gatgaccacc acagaaagtt tatcggtgta acctgaatca aggtcgagat aaaaattgga
    21361 aagccccgaa gccaactgcc caagcagatc gccgatgtag ccgccgtttc catcggtttc
    21421 gtattcgtgg gtgtcccagc cgccaaaatc cacggctgcc acacgcatcc ctgcatcaag
    21481 tttgatcatg cgggcgatcg ttttcaattg ctgaccgaat tcatcgtcgt tgtagaatgc
    21541 gccgttcgcg ggttggtatt cctggctgac caacgggctg acaatatcga gcgcatcaag
    21601 cgtgcgcaag cctgcgcggt gcaaaagcgt ttcgccctga tacatctgcc gcagggcggt
    21661 ttgctgtgaa ccgatcaaat cattgcccca ttgagaaaaa tcttcaggcg tgctcacgtt
    21721 gacggtcggg acaaaattca acagcgaagt gggctgcccg gttgtggaaa ccacaggcaa
    21781 aatggaggaa acccctgtct cctgcaagtg gcgcgtgatc caacctgagg tggtgttctt
    21841 cgagcccggc gttcccaact cgatgtattc ctgcgcatcg aagtgactgc gcgtatcgtg
    21901 gttcagccca acggcatgaa ccacacccat tttgccagcc tgatacaact catgcagcgg
    21961 ggcaagtgca gggtgcaaac cgaactggtc gttgagcgaa agcaaatttg agatcttgat
    22021 atcggggcgg gctttttcgt aaaagccgcg gtcatcgcct tgcaatggcg gcaccacatt
    22081 gagcgcatcc caaccgccgc gcaaaaacac aaccaccagc gtatccgacg gattatcctg
    22141 ttttgcaaag gcgaggttcg tcaaacgcgc gcctgccatc gaggcaatgg cagcggaaca
    22201 accttgtaaa aactgtctgc gggagattct aggtgcgggc atggtttctc acttccactg
    22261 aaaatcgggg ctcatcaaaa tcacttccac cattgcaggc agcacatacg ccacatggtc
    22321 ttcgggcaac ggttcctgcg tcccgtattc ctgtgccatc acagcgatga ctgagtttct
    22381 atcggcatcg gaggaaagcg gacgaccgag gatgcgttga atccaaaaat cagccagcga
    22441 ctcggcggtt cggatctccg ccggggtctg gttcaacaaa tctgtgtgca gggtcagccc
    22501 cttgtcatcg tcattgagcc agttttcagc cacccccaca gcgaaattcc agcgatacaa
    22561 aagggacatg gaatttgccc acgcctcctt cacatcgggg taaccatcgg gtgaacggcg
    22621 ttcgaagagc ggctgaccca tcaggctcag catccacggc acgccgccgg gaacacgggt
    22681 gaaatcggaa ttcaacgacc gcagggtcga aatacaagcc tccagcgggc gcttgatctt
    22741 ctgtccccaa ctctgcttga attcatcaga gaggagcaac acttccatca cacgcttcaa
    22801 ctggtcgggc gcgtcgcgga attctgtaaa ggcggcggcc actttctcta caacagaatc
    22861 aggcggcagg tcggcgatga agcggcggca taatttccgc gcaatgtggc gggcagtgcc
    22921 cggatgcgcc gcgagcaagt ccagcacatc ttttccatcc tgcatatcgg gttgatcggc
    22981 agggatatat tttccaagca ccagtttgtt gaaacggtcg tgccagggtt tgtaatagat
    23041 gaaattgcct gtgatgccaa caccctcctc ccattcgccg aggtcatcat caatgcgcca
    23101 gcccgtgagg cagcgcgcgg cttcgtacac atcgttatcc acatagccga tggcgattcc
    23161 gttcgcatct ttttctaccg tattcgggtc gcgcacaccg agataatttt ctgcgccgag
    23221 ggtgtgcagt tcaaaaagtt cacgggcaaa attttcgttt gggccagcat cactgctgtt
    23281 gttttgattc aggtaataca acatggaggg atgggatgcc accgcttcga gcatctgacg
    23341 gaaattgcca agcatgtgtc ttcgcagcac atcgcggttg tatgaaacca gcaatggcac
    23401 gccatcatcc tgccagaaga aaatgttgaa atggttgtgc cagaaatcgg ccagcaattc
    23461 aacaagttgt tttttgctga acacagcacg gagcagggtt gcatccacaa gttcataggc
    23521 aggcagactg taccactccc aataggcatc gtcattggca tcgtaggcgt tgttggcaat
    23581 atggtcggag taaagttgtt cgtgtgattt gtgcaatgcc tgaaagcccg ccgcactgta
    23641 acgggattca aaatcgctgt cgtcgagcga atcgggattc agttgctgtt gcacaaacgc
    23701 ccgcagacgc tcatcgtccg tgctgccaag cgcgttgaag gcttcgatat ccccagggcg
    23761 aatcccgaag gtcatgcgcc caagcgcaag gatcgccagc gaaacaggcg gcaatgaaac
    23821 cgcaggctca gggatggaaa tatcatttgc gctggtggtg ggagagagtg gagcagtgga
    23881 ggtttcttcc ccgggttggc aggcggcaag cagggaagct gttgcaagtg agcctcccaa
    23941 tttgagaaaa tctctgcgtg aaaattgttt tgaattcaag acgcctcttt tctctttcgt
    24001 tctacaaagc aaacacttca cgaaatgcgc gggtcacttc ggctttcacc tgttgcatcg
    24061 aaggcgcggg gtcaagcagg tcagccagcg agacaacagg ctcattcaag ccgcaggcga
    24121 tgatgccatt ccaatactcc atatcgggct ccacattcaa cgcaaaaccg tgacgggtga
    24181 tcccgcgtgc atccactttg acgccgatgg cggcgatctt tgcaggcttt ttgcgatctt
    24241 ccggcgaaca gcgcgggcag cgcgaatgca catcagattg gatccaaacc ccggtcaacc
    24301 ctgaccgttg gcctgataca atgccaaaat gcatcaacgc cgtaatgaga gtctcttcca
    24361 acttgcggac gtagccgaca taatctgctt caggcagttt attttcatta tttaattttc
    24421 cgagtgataa caggggatat cccaccaact gcccggggcc gtgataagtc acatcgccgc
    24481 ctcgatccac ccaatgtgtg gcgatgcctt tttgtttcag ttgttcttcg ttccaaagca
    24541 gattttcggc atgtcccttt ctgccgaatg tataaacgtg agggtgctca agcagcaaga
    24601 gcgtgggcgg gcgtttcccc tcagcaattt ccgccgcata atcatcctgc aacttccagg
    24661 ctttttcata ttcgatcagc cccaggtctt cgatggtaaa agggaacata taagaatgaa
    24721 aacaataaat aaaaagttga gaatgatttt gaacgaattc ggaattaact gaatatgcgg
    24781 cggtataaat ctgattcgag caggccgttc gggtcgcagc gttttttcag tttcctgaat
    24841 ttttccacgg tctcttcgcc atagaaggcc cgtgcagttt cggcactggt ttcgctgttc
    24901 ttggcaaaat agaaccgacc acccgcattt agcacgatct tgtcgaaatc ctgcagcatg
    24961 gcactcagcc ttacgcgagt ccgattcgtc actttgaaat cgagggccag ggagaatccg
    25021 tccacagcat gggtgagcag gaatttatcg gggcggtgcc gcttggtgac accgaggtag
    25081 gaggggagtc cacgcttttt ggcgagggcc agcatctcgg tccatgcttg atgcgcggtt
    25141 tccttcggca ggaaggattg atattggatg agtccgcctt tgccatagga aagttcccag
    25201 agcgggacat aatccaaaag gaagtgaaag gcggcgtggc tttgacggaa ggtatgtttg
    25261 cgcaggctgg aaatatattt ggcggtgtta accagacccc agccaagatt atttgcaaac
    25321 ggggtcatga aataatgcag catggatttc ggcatcacgc caaacaaacg atctggcagg
    25381 tgttgataag aaagctgcat ggtcttttgc gcttcaggat cttcgccttc gtgcaggtaa
    25441 tttgcagcat ggatctgacc atgcccgatg gtgttgatcg tatcaagcca gccgacaatg
    25501 taatcgtaat tcggggcgcc atcttcaagc gaggccagat gatccttaag tgtgtgtgaa
    25561 ggccaggcgt gaacttcgag caggcctgaa tgcatcttct tcatttgcat tgtgaccgaa
    25621 gtaaaaatac ccaacatacc cagcccgctg atcatcgaat ggaacaggtc gccatttttg
    25681 tttggggagc aggttacttc tgcgccggtg ggaagcacgg cggtaaattc caagacgtgt
    25741 tcaccgatcg tacccattct aaaattattc ttgccgtgga tattggccga aagacagccg
    25801 cccaaagtgg tgaacatggt gccagagacc acaggcggcc accagccatc aggcagaacc
    25861 ttctgccata attgtttgag cgtaacccca ggctcgcacc gcacctgacc tgtggcaggg
    25921 ttccattcca gaatctgatt cataccggaa gtatccagca caattccacc gccatttaaa
    25981 gcagcgtcat tgtagctgcg accaccgccg cgtgctgcaa ccgtaaggcc catctgtttt
    26041 gcagaacgga aggcttcgta aatttcttcg gatttttgag gtttgatgag ataggaggga
    26101 gctttgagcg aatgcccaaa gttttcgagt tgggtaaaag tattgttcat attttctttg
    26161 aatacaccct caaagtgaag ggccctttgg ggtcggctaa aaggacaggc ggcggaagat
    26221 caccgatggc acatgttgaa tggcaagcat gatccagcgc cagatggatg cggtgtagat
    26281 caattgcttc cgcttgcgca tggctttgta aatatcttca gcagcttgtt caggagagat
    26341 cgcaaaggga gtgcccccct gtgcggcctt gagcatttcg gttttgacaa agcccggctt
    26401 gacggttaat acattcacac cgtggcgcgt caggcgattg cgaagcgctt cgaggtaggt
    26461 ggtcaatgcg gctttggagg tgttgtaacc ggggttgcca acacggccac ggtcaccagc
    26521 cactgaggag attcctacga tctgtcccgc tttcgcgctt tggaacatct cggctaccgg
    26581 gctgagccag gccatggcac caatgaggtt gacctcgatc atcttgcggt caccttcgaa
    26641 attgtaactg ttcaaaccgg gggggaagtt gacacccgcc atgaatacga gcagatccaa
    26701 cccgccgagt tcggcggtaa tttttcgcaa aaggttggga acctcgtcgt agttggaaac
    26761 gtcatgggcg tacgcaagtg cacgggcttc gccttcagca gcattgattt cggcacaaag
    26821 gctggcaagt ttgtcctgtc tgcgggcgat cagggcgagg gtgtatccct ctttggcaag
    26881 tttacgggcc agagcagcgc ccattccatc agaagcgcca acgatgattc cccgtcggcg
    26941 cggattaagt ggtgttgctt gagcaggctt gggtgagttt gacacgcgag tcctcataaa
    27001 aacaatatgg atgttattct aacacaatcc aaatctgaga aaaaaaggct atataatcgg
    27061 gatattggcg gtcattttag aggataccag ccatgaaaga aacggtcttc tctgaatatg
    27121 aactggatac atgcaggttt gtcttgcgaa ttccagtaca aaaggacggt tgccccccgc
    27181 ccatcgtcat gataaaaagg tgttcactat ggctggaata acggaaaaac aaaaaaatat
    27241 ctttcaccat gcacgggaga tcatcaaaca ggatgaatcg atcccggaag aggagaaaga
    27301 gggcatcttc aagttgatac gggtgcttgt agaggttgca gacctggcac cttccaaact
    27361 gccaaaccag gaagaatccc ccaccgcctt cacacaggaa atcatttccc gccatgcatt
    27421 aatggctgct gtaaaacagc agatcgatga gcttgagtct cttaaaaaac tcagcctgaa
    27481 tgtgacttca agccttgacc tgcaaaccgt gctggatacg gtggtcgcag aagccatgag
    27541 gctggtaaaa aattcacgtg cggcccacat ctttttgtat tcacacggaa aactggaatt
    27601 cggttcttca ctcactgctg aaggcttaaa aaacatgcca gtggccatgc cacgcaaaga
    27661 ggggttaacc gcaactgtgg tggcgagtgg agttccgatc attgtggaag atatcaccga
    27721 ccatcctttg tacaccaacg tttcccaaaa ttggcgcggt tccatcatcg gcctcccgct
    27781 taaatacaac aaatccattg tgggtgtgat gaacctctca cgctccacag tcggtggttt
    27841 tacacgggcg gagttacgcc tgatcgggct ggtagctgac ctggccgccg tagccatctc
    27901 caatgccagt ttgcatcagc aggtggccgc gcaagccaat acggatagtg ttacaggcct
    27961 gcccaaccgc cgcgcgttgg acgaacgtat tcaagaggag atccgttttg caaggcgaat
    28021 gaacagcgaa ttctccgtag tcatgatgga cttggatggt ttcaaggctg tgaacgacac
    28081 ctacggccat gtggttggcg atgaattgct gtactctctc tttaattatc ttgcagagaa
    28141 catccgcccc actgattttc ttgcccggta cggcggtgat gaactgaccc tggtcagccg
    28201 tgaggccaac caagatatgg cggaaagcgt aacacaaaag atcattgacc tcgtcagagg
    28261 gtacaaaccc gcatttgctg aaaagaagaa cattcaactc ggaattacag caggcattgc
    28321 catttatccc acccactcca aaactgcagg tgacttgctc cgtgccgccg atgccgccct
    28381 gtatcaagcc aagaaacacc atcgtggttc ctattccatt gcgaagatca ccaccggtcc
    28441 cctggatccc atcaccctgc cccgcagaaa gcctgaataa aaagatccaa accgaccgac
    28501 tcacaaatcg tcctcctgca accaaacaga taaagatggg gttatattgg taaatccaat
    28561 cacgggaggc agatatgccc cgcaaagtaa aactcatcct caaccccatg gccgacatgg
    28621 gacgcgcctg gaaaaccgcc aatgacctgc gcccgatcgc acaggaattc aaaggcgaat
    28681 tgacctggag tggaacggtc tacccgacgc atgccatcga gctcgcccgt caggccgctg
    28741 aagaaggtta tgacctcgtc atcgccatgg gtggcgacgg cacggcacat gaagtgatga
    28801 atgggctgat gcaagtcccc gcagaaaaac gacccgtgat gggagtcgtc cccattggct
    28861 cgggcaacga cttcgcctac tccatgggca tcacccaaaa atcggcacac gccctcgcgc
    28921 acatgctcaa agccgaaacc attcaaagca ttgatatcgg ccagatgacc gacgagcaca
    28981 accgcaaaga atatttcgac aacacactcg gcatcggctt cgacgccgtg gtcaccatcc
    29041 gctcgcacaa actgcccatc gtcaaaggct ttttgatgta tctcacggca gtcattcaaa
    29101 ccatcatcct caaccacaac cccgcacaca taaagatcga aaccgaatca gagcaatggg
    29161 aaaccagcac actcatggtc acgctctgca acgggccgcg tgaaggcggc ggctttatgc
    29221 tttctcccaa ctccaaaaac gacgacggca aaatggaata cgtcatcgtc aacaaggtct
    29281 cacgcggcat gatgttccgc ctcgtacccg aattcatgaa cggcacacac atgcgcttca
    29341 aacaaatccg catgggcgaa ttcaaaaaat gcagcatcac ctcagatcgt ccgctttaca
    29401 tccatgccga tggagaaatc ttcaccagct ttggcagcaa cctgcgcaaa gttaatttcg
    29461 aaattctgcc gcaggcattg aaggtggtac gcggttaatc actcgctact ttccactttc
    29521 tcctttagaa tatgaaaatt ggaaagtaga aatgccaagc ctttaccaaa cctttctcaa
    29581 acacgacctc ggacatcttc gcatcgtagc tgatctgtgg ggactcgaac tcgaatcgca
    29641 agatctggat tcagctgccg aagaactctc cgcatctttg ctggacctgg aagctgtcac
    29701 tgaaaccatt gacattctct cccctgaatc acgcgccgcg ttggatgcca tcgttgaatc
    29761 caacggcaaa atggaatggg cagccttcac ccgtgctcat ggcgaaatcc gcggcatggg
    29821 cgccgccaaa cgtgacaggg aacgtccgca cctgaaaccc atctcccctg ctgaacatct
    29881 cttttatcgc ggcatccttg cccgcgcatt tttcgaatcg agcaaaggct cgcaggaatt
    29941 cgcctacatt cccgatgacc tgttggaagt gatcgaaatg ctcgtggtgg aagatgacga
    30001 agcgccgaca cccgccccgc atggcgaacc gcttggccga cccgccacgc ccgtggaaaa
    30061 agccgttgaa ctccccgcca ccgatcacat cctcgatgat gcaaccacgt atcttgctgc
    30121 cttgcgcatc ggcagtcggg cagccgaagg cgtatcaacg cgcagacgcg aactgcatga
    30181 actgctcgag gcagcccatc tcatcaaaga tgacgccctg cagccagaag cggtaaaaaa
    30241 ctttctcggc gcatcccgtg cagaagcatt aaacattctc tacaaagcat ggtttgtctc
    30301 aaacacattc gatgaattgc gcctgctccc gggaattctc tgcgagggtg aatggagcaa
    30361 ccagccgcag gtgacacgcg agtttctgac caacctgatc aacgacatcc cgcaaaacaa
    30421 atggtggagc ctgcccgccc tgctgcgcga tatcaaaacc aaatatcccg attttcaacg
    30481 ccccgcaggc gattacgact cgtggttcat caaacgcgag tcagacggtc aatacctgcg
    30541 cggattcgcc tattgggatc aggtggatgg cgcattgatc aagtatttcg tccaaaccct
    30601 gcattggttg ggcagagtaa atctggccgc gcctgaggaa gggaaggaat ccacagcctt
    30661 ctttcttctt tcttcttttg ttgagaggaa tgaagaaaaa ggaaagatcg ttgtctcctc
    30721 caatggcaag atcaccatct cgcgttattt ctcacgcgca gtgcgttacc aaattgcccg
    30781 cttctgcgaa tgggacgagg aaaaaggcaa cgaatatttt tatcgcgtca cggcaggttc
    30841 actgaaacat gccaatgaac agggattgaa atcggagcag ttactggctt tgctggtgaa
    30901 atacaccaac ggcaatgtgt cgcctgcgtt ggtgaaggcg ctcaaacgct gggacacgag
    30961 cggggtcgag gcccgggtgg agagtctgct tttgctaaga gtcagcaggc ccgagattat
    31021 ggaagagatc cgcaagtcga aggcggggaa atatctgggc gaagtcttaa gcccgacggc
    31081 ggtgatcgtc aaaagcggag ccatccaaaa agtaatggcg gagttggccg agctggggtt
    31141 actggcagag gtgactacgc atgaataggg gcaggcgaat cattttgatc gttgctgtaa
    31201 ttctggcatt gattcccgtc ccgatgacat gcgggcacat gttttacggc tgtgccaccg
    31261 ggccagatat gagcgggaat tactacacct attacgaagt tgagcctttg ggaatcacgt
    31321 tgatcgaaac cctcattatg accaatttga aaatctatta ctggagcgga aaagaggcgc
    31381 atttcatagg gattccatga cacatgacgc gggggaaacg catataataa aaatgtcatt
    31441 tcacaagcaa tcaggagaaa aacaaatgtc taaaaccgac tggattcagc aggaaattga
    31501 taatttgaaa tcacaggggt tgtataacaa catccgtacc atcggctcac cgcaaggcgc
    31561 atggttgacc gtggatggaa aaaaggtttt gaacttctgc tcgaacaatt atctgggatt
    31621 ggccaatcac cccacactga ccgccgccgc aaaaaaagcg accgatgaaa tgggcgttgg
    31681 ccctgccgcc gtgcgttcga tcgcagggac catgaccctg catgttgagc tggaaaaacg
    31741 gcttgcacaa ttcaagggcg tggaagcggc gatcacattt caaagcggtt tcactgccaa
    31801 tttggccacc gtgcctgcgc tggtcggcaa agaggatgtg atcttttcag accggttgaa
    31861 tcatgcttcg atcatcgatg gctgtcgttt gtctggcgcg aagatcattg cctatgaaca
    31921 taacgatgtg aaatctttgg aagagcagat caaggcgaac ctgagtcaat atcgccgcgc
    31981 tttgatcatc accgacggcg tcttcagcat ggatggtgac attgcgcccc tgcccgatat
    32041 ttatgaagtg gcgaagaaat acgacatttt attgatggtg gatgacgcgc acggcgaagg
    32101 cgtgcttggc aaaggcgggc gcggcattgt agatcacttc ggcctgcacg gcaaagtgga
    32161 tgtggaagtc ggcacgatgt cgaaggcatt tggtgtggtc ggcggaatcg tggcgggcaa
    32221 atcggtcatc atcgaatggc tgcgccaacg cggacgtccc ttcctgttct cgtcggcggt
    32281 cactgtgcct gatgcggcgg cctgtctggc ggctgtggat ctgctcgaag attccacgca
    32341 actggtggat aaactttggg acaacgccaa gtatttcaag gccgagatga agaacctcgg
    32401 cttcaacacg ggagtcagcg aaacacccat taccccgatc atgctcggtg aagcgccgct
    32461 ggcccagcaa ttcagccgtg agttgttcga agcgggcgtg ttcgccatgg cgatcggatt
    32521 ccccaccgtg ccgcaaggca aggcgcgaat ccgtgtgatg atctctgccg cgcacgcgaa
    32581 ggacgatctc gataaaggtc tggaagcgtt caagaccgtt ggaaagaagt tgggtgtgat
    32641 ctaatcacaa tggatgcaaa gagaccgcaa agtgctttgc ggtctctttt ttttcaggtg
    32701 acgtataatt tggaggatgt gggtatcaga ctgacataaa tggtcagcga ttatttggag
    32761 gtaaataaaa tgaatgttcc catcttccct gataatatca atctctcccg tccatttcac
    32821 ttcacaaccg agaatgcaga cagtaaagcg aggattggtg gcaatatgcc cgaagaattg
    32881 tccatctccc tcaatgaaaa tgcccaatat ttcgggacgt tcccccttta tgacaacgag
    32941 tcacctttat atttttccct gtttatcaat tgttcgttta aagagttcgt tgtagattgg
    33001 cacggtagaa tcaaagatga cagcaaagta gtggtcgctt tgcatgaata tcgtccacgc
    33061 agcaagtcca tgcaatatgc ttcgagattg aaaagccagc attccattga aattctgaaa
    33121 acacgcgaag atatctatca aagcgacaca ggagagcttg tcgttcggga aggtcacaaa
    33181 tttggcggtg caccttactg tatacaggaa ccaaaactta tcggctggga ggaactacag
    33241 gcacaggggt acaggcaaat cctgcagata gattttccgg ggttggagga tggcgccgtt
    33301 tcaggtacct ggccctttgg cgatggcatt ttcaacttat tttggaaata tccattcgaa
    33361 ggcgagaagt atttttggtg cgtacaaagc tgagactcaa tcttcaatcc ttttattgca
    33421 tcatatccag ataggattta catccgtcag caaaaattca aaggagtttt ctgccttttc
    33481 tttttagacg cgatgctcaa accaaaaaaa ccttcaaatc acaattccaa gctgaaagga
    33541 tgcacaaaaa aacatgtcca ctgacccaac attccgcatg accatacagg atgttttttc
    33601 catcaaagga cgagggaccg ttctcacagg ccgaatcgag agcggaacaa tcaccgtcgg
    33661 agatgaaatc atcatcaaag gcaggaattc aagcaaaaaa gcgatcgtcg ctggtgttga
    33721 agtgcgccgc aaggtcacaa ccagcgcccg gtctggcgat gaggcaggca ttcgcttgaa
    33781 agacaccgaa gaagacgttc aacaaggtga catcctgact ggctccgagc atgatttcac
    33841 ctggaagccg tagagcagat tcaggttaat ggtaaacgtt acaccactac aaataaaaat
    33901 tctgggatta cggtacggtt ttctctcctt ttggccatgc ctgccgtccc cgccctccgt
    33961 caaacctcca atggtataat cccgacgaaa atatataatt ccccacaaaa gtggttgaaa
    34021 aattgaagga gaacgtcatg tcaggtcatt caaaatggtc aacaattaaa cgcaaaaagg
    34081 gcgttgccga tgcaaaacgc ggcgccgtat ttacacggct ggcccgtgag gtcaccatcg
    34141 ccgcacgtga aggcggcgac cccgatacca acttccgcct gagattggca gtggataagg
    34201 cccgcgccga gaacatgccc aaggataata tcgaacgcgc catcaaaaaa ggcacaggcg
    34261 aagacaagga aggcacaacc ttcgaagaac tcacccttga aggctacggc ccgcacggct
    34321 ccgccctcat ggtcacctgc gtcaccgaca accgcaaccg caccgtcccc gatatgcgcc
    34381 acgccttcag caaatcaggc ggtaacatgg ccgaacctgg cgcagtcgcc tggcagtttg
    34441 accgcaaatc ctattttgcc ttccctgcca gcaaactctc cttcgacaaa gccttcgagc
    34501 tcggcatcga agcaggcgcc gacgatgtcg tggatggcgg cgacaccatc gaaatctatg
    34561 cccccgtcga atcattcaag accatcgccg atgccctcca caaggttcat gttcagcccg
    34621 aagaggccag cctgcgcatg atcccgaaac aggaaatcga actcgatgtg gaatcgaccc
    34681 tcaaattcat gaaaatggtc gaaaccatcg aagacctcga tgacgtgcag gatgtcttcc
    34741 acaacgtgaa ggtttcagac gaagccctcg ccgccctcga agctgcataa tcaaaatcat
    34801 ggtagggaca cagcggcgct gtgtccctat attatttaat gaccctcgcc ctcggaattg
    34861 accccggcac cgccacaacc ggatacggac tcgtgcgcct catgcccaac ggggaactgg
    34921 tcacggtttc ctacggcatt atctccaccc caaaagacgc caccccgccc gcacgcctcg
    34981 aaatgctttt tgatgacctc aacaagctgt tgaaaaaaca caaacccgaa accgccgcag
    35041 tcgaaaaatt attcttctcc aaaaatgtca ccaccgccct ggccgtaggg caggcccgcg
    35101 gcgtgatcat gctctgcttg caaaaagcag gcatcgaacc ttttgaatac acccccaacg
    35161 aagtgaaaca agccgtggca ggctacggca gcgcagacaa acgccaggtg caggaaatgg
    35221 tccgcgccct gctgcaactg gattccatcc ccaaacccga tgacgccgcc gatgcattgg
    35281 caattgcgat cactcattta aatacaaaac gatacgagta gggacacggc gctgccgtgt
    35341 ccctttttca aatggcataa cggcgttgtg cccctaccca cacaatacgg taaaatacaa
    35401 atatgatagc aaccctgcgt ggagaaatca cccaaatcga agagaacgcc atcgtggtcg
    35461 aagtcggtgg tgtgggcatg cgcgtctttg tccccgcccc attgcggacc aatgccaagg
    35521 ctggcgaaat tctttttatg ttcacccatc tcgtcgtccg cgaagatgcg ctgaccttgt
    35581 acggattcga atcacaagcc gaccgcgacc tgttcaacat cctcctcggt gtggatgggg
    35641 ttggccccaa agtggcgtta tccgtcctct ccaccctcac cattgacgcg atccaacgcg
    35701 ccatcttcgc cgatgaagcc gacttgctca acagcgtacc cggcgttggc aaaaagaccg
    35761 cccaaaaaat ggcgctgcat ctcaaagaca aactcaaacc caccgacgca cttggcaaaa
    35821 tcgccgccat ggccgattac gacagcgaag ttctcgccgc gctcaccgca ctgggttact
    35881 ccgtcgtcga ggcccaggct gcgatacaat ctctgccaaa ggatgcgccc aaagaagtgg
    35941 aagaacgctt acgcaccgcg ctgggatatt ttcaataaaa aacgccaagc ctccgagata
    36001 aaactcggag gcacttttat aaacacaaga gtaaaataac caacaccaat tttcaacttc
    36061 tccctttccc tttcttcatc attcatactt catcattcat cctttgacct taggagcctt
    36121 ctcatgctaa aaaagaaaat cgccttcatt ggccccggtg tcatggccga agccatgatc
    36181 gcaggcctgc tgcgaaaaaa actcgccgat gcaaagaaca tcaccgcctc cggtccgcgc
    36241 gaagaacggg gcgtggaatt gaacaagaag tacggaatca aagccaccac cgataacgcc
    36301 gccgccatcc acgaagcgga tgtggtcgta ctctcggtca agccgcaacg actcaccgaa
    36361 gtgatgaagg gtctcaaagg catccgctcc gatgcgttgg tcgtgtccat tattgcgggc
    36421 gcaaccatga aaaagatcag cagcggactc aaacacaaga ccattgtgcg ctccatgcca
    36481 aatacgccgg ggcagatcgg cgaaggcatc accgtatgga ccgcatccaa agaagtgacc
    36541 gacgaacagc aggaaatgac ccgagccatc cttggcgcac tgggcgaaga agtcttcgtc
    36601 gaagatgaat cctatttgga tatggccacc gcgctttcag gttctggccc tgcctacgtc
    36661 ttccttttca ccgaagcatt gatcgatgca ggcgttcaca tgggctttcc gcgccgcatt
    36721 gccgaacagc ttgttttgca aaccatcaaa ggttcggcat cgtattatca aggcgcaagc
    36781 aggcaccctg ccacactgcg caatcaagtc acatccccgg gcggcacttc ggcagaagcc
    36841 ctgtattatc tggaaaaggc gggcttccgc acggcgatct cacgcgcagt atgggcagcc
    36901 tatcaacgtt cgctcgaact cggcaaggaa aaaccaggtc acattgatac gcacgacgaa
    36961 gaaagcgcgt aatctttatc aaccgcaaag gacgtaaaga tttaaaatat tttcttcgcg
    37021 atcctggcgg ttcaaaatga aaaagacaat caccctctta accagcggca cacgcggcga
    37081 tgtgctgccc tacattgcgc ttggcgaagg cctgctcgat gcaggttatc aaatccgcat
    37141 tgctgctccg cttggttttg cgaatctcgt tcaacaaagc aaactcacat ttgcgccctt
    37201 cgaaggcaat ccttccgacc tgctcatcga gcagggtgac tccacgccga tgacgctcgg
    37261 cacaaatccc atccgctcca tccgtgctac gcaaaaattc atcgggcagg ctcgtccgct
    37321 gtatcggcgc atgctccata ccgcagccga agcctgccgc ggttccgata tgcttattca
    37381 tggcctgccg accatttggg gcgcgcatat tgccgaaggg cttggcatcc ccgccgtccg
    37441 cgctgtgttg cagccgctcg cgcccacccg cgaatttccc tccgcgcttt tgccgtttcg
    37501 ttcttctttt ttggggatcg gaaactggct cacccattgg gcagtgactc aagccacatg
    37561 gctttcgtgg cgccgtgaga tcaaccacgc gcgtcactcc gactttgggc tgggtgccgc
    37621 tcactggctt gatccttcgc ttcgggctga ttcaaaccaa cccctcaccc tgaacggatt
    37681 cagcacacag atcgttccac gcccgaaaga ttggaatgac aagcaggtgg ttacagggta
    37741 ttggagaaaa aacgagttga ccaataatca cgttgacagg ccagaccttc aacattttat
    37801 tgattcttcc cccaacccca tcgccattgg gtttggctct ccaggcacac gcggatattt
    37861 accgatgctc gaagaagctt tgcagcatgc caatgcgcag gcgattctga ccatccctgc
    37921 caaatggcat ggcgaaatca aatcaaaaaa tatttttcct atcgaatatg caccacacga
    37981 ctggttatat cgccgcgtga aggtcgccgt ccatcacggc ggggcaggga caacttctgc
    38041 gagtttacat gcgggcattc caacggtcac actgccgttg gcgatcgacc aattcttttg
    38101 gggggaacag atccataaaa tcggcattgg gccgaggccg atcccccaaa gaaaattgaa
    38161 cgcggaaaat ctttcgcaag ccatcacaca ggcattgaca gatcatgcca tgaacgcgaa
    38221 agcaaaaact gtcagcgccg agttgaacaa agaaaatggg attcaggccg cggtgagtca
    38281 gatacggaaa ttactatgac gtcgcctcaa tgggattaat ccaaaactgt ctggtgagat
    38341 gcatttcccc gcaatgacat atacgccacc cccaaagcgg tcaaagaaaa aatactcccc
    38401 acccaatacg gcgcagaaaa agcaatgacc tcaaacgaaa cacccgcaag catggggccg
    38461 aaaatatttg taagtgaaag caaggtttgc gtaccgccca tcaaacgtcc ctgttcgctt
    38521 tcagagacgc gcaacgaaac caatgccgtg agggatggaa tgctcgtgcc tgtgcccagt
    38581 gcaacgacgg tcacagcggg gtaaagcatc catgcctgcg gggcgagcgc catacctgca
    38641 agaccgatga ccatgagcga aaggccaaag aggataaggc gtttttcacc gaaacgcggc
    38701 tggagtttgc cgaacaaaaa tccctgaatg aaaacagcgc acacgccgac atacaggtaa
    38761 aaaaagctgt tctgtgtggg tgtccaatga aaacgaaaat tggaatacag cgggaagttg
    38821 gattgcagcc cggcaaaggc aaggttgagc agaaacatcg tcacaagaag tttttgaatg
    38881 ttggcctgat tgaacaaacc gcgaaaatgc ttcatccagt tgaacatttc tgaaaccgat
    38941 ctggcttcgc gtttttccag cggaagcgat tcgggcagca cgaaataacc aaagatgaca
    39001 ttgcccagcg cgatcgcaga tgcagtgaat gcgggaacgt acaagccgta tttgctcagc
    39061 aatccaccaa gcgcgggtcc tgccatcatc cccaacccaa acgccgcacc gaccatgccc
    39121 atgccgcgcg cgcggttttc ggaggtggtc acatcggcga tgtaggcata ggcagtggtg
    39181 aggtttgcgc ctgtcaatcc gtcaatgaaa acggcgagaa aaatcagcgg aagtgtatcg
    39241 gccaggccaa gcataaaata ccccagcgaa gtgccgaaca ggcaaaccaa tagaacaggt
    39301 ctgcgcccaa accgatcgga gagcgcaccc aggatgggcc cagcaaataa ttgcgtacct
    39361 gcatagagcg aactcaacgc acctgctatt gcagcgcctc catcttgagc ctgcacgtaa
    39421 aaaggcagca acggcagcat gatgccatag ccgagcaagt ccacgaaaat ggtgatgaag
    39481 ataaaaacga gggaggaatt caaattgtta tcaaccgata aaaagcaggg aaaagtggaa
    39541 agtggtgagt ggaaagtaat cactttctac tttccacttt ccatctccta aaatctctcc
    39601 aaccgcagcg gactcagatc gaacgccgtc tctttcccgt tgaccagttc cgccaccacc
    39661 tgccctgttc caggtccaag ggacagaccg agcatggcat ggcccgtggc aagcacaagg
    39721 ttggaatgat tcggagagcg gccaatgatc ggcacgccat cgggcgtcgt cgggcgcaac
    39781 cctgcccagg tttccttgat gtcgagcgtt tcatcaatac ggagatattc gcgtgcagaa
    39841 ttctcaatcc gttggatcca gcgcgggtcg ggcgtcatac tgccattgcc aacttcaagt
    39901 cggcctgtaa aacgcagcag gtcacccatc ggcgaaaccg ccacacggcg ctcgccaagg
    39961 atcaatgcat gacgcggcat ctgcttcgtt gcagacatgg tcaggctgta tccacgcgca
    40021 ggctggatgg gaagattcaa tttcagatcg cgcgccatgg atggagtcaa cgcacctgcg
    40081 gcgagaatga catgctccgc ctcgtgcttc gcatcaccga cagctgttct tacaacccga
    40141 acttttccct gcgccgattc aaacccagtg acaggcgtat tctcaagcat ctccgcgccc
    40201 atatcgcgga cacggtcact gagcattttg agaaataacg cggggttcat atgtgaatcg
    40261 cctgtaaaat gcacgccgcc gatgacatca tccaatgcgg caggctcaat ctcgtgaact
    40321 ttggccttgt catacacgct gacggggatg ccatgcttct gcatcaaatc accttcgtgt
    40381 cttccgccgc tgaaggcttc ttccgtttta tacaaaaaca gcagaccctt ctcctgataa
    40441 tgacagtcga atttttcctc ggcgatcatt tgggcaaagt tcttcgcact caattgaccc
    40501 aggctgttca atggcggaat tccgcgctgg acgttcgatt cactgcaagc cagcacaaac
    40561 cgcagcagcc acatgagata ttttggatcg agactgatct tcatcccaaa cgggctgtga
    40621 gcagggtcaa gcatccactt caacgcggaa gttaccacac ccggcgctgc caacggaata
    40681 atgtggctcg gcaccacatg ccctgcattg cctgaaccgc tgcctttgcc gatctctttt
    40741 gaatcaagaa tggtcacttt tctgccagat ttcaaaaggt aatacgcgca gcttaaccca
    40801 acgggtccgc cgccgatgat gagaacatcg tgctgttgca catctgattt tgtggtcatt
//
LOCUS       DAOQLC010000020        40426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_278_length_40426_cov_11.822868, whole genome shotgun sequence.
ACCESSION   DAOQLC010000020 DAOQLC010000000
VERSION     DAOQLC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 40426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_40426_cov_11.822868"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PL000_05405"
     CDS             <1..551
                     /locus_tag="PL000_05405"
                     /inference="COORDINATES: protein motif:HMM:NF019123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HNC88363.1"
                     /translation="ESDRYLFKFADGATFTILDKWTSKSTTVWGDPHVDVDDVQGNSN
                     GDFKDLKASDEFTTFMLSDGTRVTFKARDAGIIEQVDIFKGSQHVRGIGQAAEGYSPE
                     SGLFLQTVLQDGKTAAISTPSGDTVLSGGDGNDWYDSRGNLVWGKTTGPVVTQRPNAT
                     LEFMYRQTVSQSVSVKTISKDA"
     gene            711..920
                     /locus_tag="PL000_05410"
     CDS             711..920
                     /locus_tag="PL000_05410"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNC88364.1"
                     /translation="MIFLTRTNGAIFYINPELIQTVEATPDTVVTLVNNKKLIVKDTP
                     REIAERFIEYRRKTLAPFDAPTSGR"
     gene            970..1803
                     /locus_tag="PL000_05415"
     CDS             970..1803
                     /locus_tag="PL000_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HNC88365.1"
                     /translation="MDIATISGLVLALTAIITALILDGGSPAELFAAPAAIILIIGGS
                     IGATVVTSPLKIALKLPQFIMQAFKTNKFNSKGDIELLTKLADKARREGLLALEEDSR
                     KLTDKFLKKGLMMVVDGAEPEQVSAIMENTIDQMRTRHKQGIGFFTAAGGFAPTFGII
                     GTVMGLISVLKQLDNPSALGEAIASAFLATLWGLLTANLVYLPVAGKLKAKSEEEAHN
                     RYMQLEGILSIQAGENPRIVRDKLMAYLAPSEAQGEKEGDKAKGADKKAPAANTGKAQ
                     A"
     gene            1806..2576
                     /locus_tag="PL000_05420"
     CDS             1806..2576
                     /locus_tag="PL000_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNC88366.1"
                     /translation="MAHRKEDHEEHHDERWLVSYADFITLLMVLFVVLYSMGQVNVEK
                     YKQLAESMRTAFTLGGASQVVDSQINQAGGTSEDGSSKPIVVPGIPEGPTQSEEVAGQ
                     LTSMLSSQNLGNQVSVQTNIEGVLISLSERLIFKNDQTELPAESLQVLDTIVEMLRPI
                     DNKVRLVGHTNNTPPTNPAYPGNWELSLARATSVAKYLINSGISPERLIISGQGEYAP
                     IFPNDTEQHKELNARVEIIIIYKVDSNIIDITSPVIIP"
     gene            2604..3191
                     /locus_tag="PL000_05425"
     CDS             2604..3191
                     /locus_tag="PL000_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNC88367.1"
                     /translation="MLDKILKILNVITQVILVITAIISLITAYVIFAPDELPKPFRLV
                     YDYQTTLTTPLAPATEAAVEATEAPQETHEFTPGEGIMLNMSTKIINLLDPGGRKYIR
                     VTIVVEFAPDNPEYESLPEEEKTAYLTAFEEKLNSRLPIMDDTVITLLSTKTYEDLYT
                     AEGKEKLRMEIQQTLSEKLTDLHIISIYFTEFVVQ"
     gene            3194..4237
                     /gene="fliM"
                     /locus_tag="PL000_05430"
     CDS             3194..4237
                     /gene="fliM"
                     /locus_tag="PL000_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNC88368.1"
                     /translation="MLAQSEIDALLSGAIDFDQKNGGDNVNLTDKVNPSAESLGPDGK
                     REKKIQPYNFWSPARFSKEQMRAVELVHEDLSERLTTSLPTFLRTNVRPRLVHIEQGR
                     FHDFIKDFPQNTLFHLISLAPLPGHMVLTMSLNVSYLILEQRLGGKIEGRFTERALTE
                     IDQSLLRGLVEHMLGDMKGAWSKVVSLEPSLEDSTVNQHWVQMMIGNERVLLLTIEIN
                     IQGVTGTMSVFIPFNTLKPITEILNPHIWIAGRKEHQQDPVARQMAIQATMKAIVPVT
                     VILGAVDLPLKELIHLAPGDVIELNTNIDSPLVVQVANKKQFYARVGKSRKRLGVQIT
                     RVYREQDEAQMGF"
     gene            4252..4605
                     /gene="fliN"
                     /locus_tag="PL000_05435"
     CDS             4252..4605
                     /gene="fliN"
                     /locus_tag="PL000_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNC88369.1"
                     /translation="MNNINTETEEMPVNKTAEPIAERPAAKSDSVNESNIELLLDVSM
                     RVTVELGRTRMKLAQILELQHGSVVELDRLAGDPVDIFVNDRMVAHGEVVVVDDKFGV
                     RITNMVSVNSEKASF"
     gene            4605..5117
                     /gene="fliO"
                     /locus_tag="PL000_05440"
     CDS             4605..5117
                     /gene="fliO"
                     /locus_tag="PL000_05440"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNC88370.1"
                     /translation="MTDLPAKIRQWIATSSPKQKLLASLATLSLLATAALINMGGASS
                     VSQDPLDSTPFYFLSAFVKLIVVLLLILGSAVIFKRWSQANPLTKKTRQLHLVESIRL
                     SPKQALHLVMIGDQKLLIGATDQSIALIAPIEGDPSLPEQETQPAASLDFGSVMRSFN
                     IPTLNAHSKE"
     gene            5126..5938
                     /gene="fliP"
                     /locus_tag="PL000_05445"
     CDS             5126..5938
                     /gene="fliP"
                     /locus_tag="PL000_05445"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNC88371.1"
                     /translation="MLNTSLPLITSPEQKKSHRPLKRPVLLIVLAFSVVLLASGCSAV
                     PGVSLTVDGQSPDPKQVTSGVQLLVLMTVLSLAPTVLILGTSFTRIVIVLSMVRNAIG
                     TPSIPPNQVIIGLSLLLTFFIMAPVYKQMDEQAIQPYINQQIDQKTALKLAQAPIRDF
                     MFKQTREKDIQLFIDMGNETQPATLDDIPSVSLFPAFVISELRTAFTMGFVIYIPFLV
                     IDLVVSSILLSLGMMMLPPSLVSLPFKLLLFVMVDGWYLLASSLVLSFAGGS"
     gene            5947..6216
                     /gene="fliQ"
                     /locus_tag="PL000_05450"
     CDS             5947..6216
                     /gene="fliQ"
                     /locus_tag="PL000_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNC88372.1"
                     /translation="MTEAYVLTLGQNAIVMALILAGPILLVSLLIGSLVSLVQAATQI
                     NESTLIFIPKIIGIILVLVLLGSWMLQHSITFTHTLFTSLPELVH"
     gene            6239..7075
                     /locus_tag="PL000_05455"
     CDS             6239..7075
                     /locus_tag="PL000_05455"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNC88373.1"
                     /translation="MPAISVEDIIRSIELLKPMPTSVSHILREVDRQDVTIDSLVGMI
                     GLDQVLTALVLQISNSASLGYSRTCSNLHDAVMHIGLRRLKSILLTSSATSMMGRSLK
                     GYRLGAGELWRHALVVGMASEWLAQVFHYPDPEQAYVAGLIHDIGKLLLDQAVLSNYS
                     RIADYVEKYQMPLWQVEEKLIGIDHARVGSLIAEHWNFPVILVDAIRFHHAPSFARGN
                     QRLPAIVNLANAFAVDYQLENSVILDSQIHPESLNILKLTPHKLEELKVSMKASGVFP
                     TR"
     gene            7080..7463
                     /locus_tag="PL000_05460"
     CDS             7080..7463
                     /locus_tag="PL000_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88374.1"
                     /translation="MKRERASILILEPNALQCDLIKLALTRHQMTPIICAQADHLRQQ
                     LAQHLPDALLIDTQLPGVNGLDLIQRLNSEVLLNRTKIFFLSSLAFPEVVQKAARMGA
                     SGFLVKPLDPDLLAARIQQSLDRPN"
     gene            7576..8706
                     /locus_tag="PL000_05465"
     CDS             7576..8706
                     /locus_tag="PL000_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNC88375.1"
                     /translation="MPDTSPNPPTRKMLLIDDDPKLLLGLKAVMTIQGFEVFSTIDGN
                     EGIRMAREQLPDIIICDVMMPKPDGFSIKRFLAGNPQTATIPFIYLTARTFTADKIAG
                     LQLGADDYVSKPFNADELVSRVEAILRRGNIGHQRGLREMEDRLEQMRNSISTNLAHE
                     LRTPLTVIMANLTMAMKEKFHGRADDMNWYLESMLSSTQKLFKLTEDMILLNDIDQGK
                     LSSKRIPINLNDGLLKPIRDVTTRYQVKNLDVQVSVQEDMLLYASENDFSRAVFHLVD
                     NACKFSPDGARIIIQLRRNGFGGCMLSVENSGSSISVELREKVFERYYQIQQGDDRPF
                     EGLGIGLTIVRAVAEVCGGSATIKDSDMGCRVLFTLPPMFEK"
     gene            8898..9656
                     /gene="fliR"
                     /locus_tag="PL000_05470"
     CDS             8898..9656
                     /gene="fliR"
                     /locus_tag="PL000_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNC88376.1"
                     /translation="MTISVAQAQLFFLAFTRIMAILVHVPVFGGQNIPSQVRIGLGLV
                     LAFVLLPWQPLPPEAAAIGTFAYSLAIGREILIGTLIGFAADLAFGAIQMAGAAMGTG
                     SGFESSRIFNPALGESGSAFDQIFVMTATMIFLVINGHHLVLIAIQKTFEAVPLNGSL
                     PFDGMETLLKTTSVFIAAGIHMAMPIIAALVLTDLTLGLLSRVAPQVQVYFLGLPVKV
                     VVAMIAMSLTFSVIFPYVSSLFKNMAESMLLFVE"
     gene            9665..10732
                     /gene="flhB"
                     /locus_tag="PL000_05475"
     CDS             9665..10732
                     /gene="flhB"
                     /locus_tag="PL000_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNC88377.1"
                     /translation="MADKTEAPTPHRLQEAREEGQVVRSQEVITAVVILSGAFLLRGP
                     GLQLGLKFQSLLVETITQLPTTEVSVESVRGMFFLYGAAILPTLAFLLFGLMLAGVAV
                     TAGQTQFLWAGKKIGFDAKRLNPIEGFKRIFSTHGLIELLRSLLKLGWVTWVAYSFLR
                     SRFFELVSLTQFDLRTSVNDFVDICLSLAIRVGSMYLVLAVADYAYQRWELYKNLRMS
                     KDEIKQEMKRSEGDPLLKSRIRGMQRRMARGRMMANVSKATVVVTNPTHLAIAIEYNE
                     GMKAPKVLAKGPYHVAEKIVQIARENHIPVVQNIPLARAIYKTIEIGQEISSDLYLAM
                     AEVLAYVYKLRGKTAFAEKGI"
     gene            10892..12991
                     /gene="flhA"
                     /locus_tag="PL000_05480"
     CDS             10892..12991
                     /gene="flhA"
                     /locus_tag="PL000_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNC88378.1"
                     /translation="MTTNTITPPAPNPIIAALQNLLRSKDIVMALSLVILVGMLLLPL
                     PAFIIDLAVALSIAVSIGIVLLTMFIKQPMEFSVFPTVLLLVTLFRLGINVSVSRLIL
                     LSGEAGQIITTFGNLIVGGNYVVGVVIFLILMIIQFVVINSGAGRVAEVAARFTLDAM
                     PGKQMAIDADLNAGLIDENQARQRRKMIEAEADFYGAMDGASKFVKGDAIAAIIIMLV
                     NIIGGFVIGAVQRGMDLTTALQTYVLLTIGAGLAIQVPSLLVSAAAGLIVTRSTSESS
                     LGTEVSAQVSNFNVLAVGALVVGLMTLVPGLPTLPFVLVSVGLGVAAYFVNREQAKAV
                     AVAEAATLLPARPTEPETPEQMLEMVVVDPIELEIGYSLIPIIDEDNQDNLLRRITSI
                     RRQLMSEMGFILPVVRIRDNLRLSPQSYRIKIRGQEVAASEIMLDRLMAIPGSDIEDP
                     LQGIPTSEPAFGLPAIWISSAEQNQAEARGYTVVTPLSVICTHLTEVVRGNAAELLSR
                     QLVQEMLNQLRQKAPASVEGVTPELLNLGEIQSVLRNLLRERVPIRDLSGILEVLANN
                     APMTRDPHILAEAVRQSMAHTISSQYKDEANTLHVFTLAPQLETALRASLVNSEGSPG
                     FGIDAGMAQKVLLKTGEQMEALAQLGHFPILICPRELRLAFRRLVDQSLPNLIVMAFS
                     EVGHGTRVKAHGMVKLD"
     gene            13000..14142
                     /locus_tag="PL000_05485"
     CDS             13000..14142
                     /locus_tag="PL000_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88379.1"
                     /translation="MVTKTFKADSTLQTLQLVQQEFGSDAIVVSMREVPEGPIWNPWK
                     KSAVEVVAAAKTPQPETVEERPEIEWELPAVKKPATLPPPLKLNLNPLPNPSAPVADP
                     VQKTESAAPTNERYLPPSLKKIQQQLTHQGVDVSLVEGLLNLALETLSPVTLADAEAC
                     KRSIVQLMGAELRVQKGAGNFISNNIVTVVGPSGSGKTSVVAKLALFFRQGLHKSVTW
                     VCADTVRTGAIAEAKAYTDALGLELKLVYTPQDLNEAFNTSQASDLYLVDTPGYNPCN
                     EKQMIELGALLSEMPSRCTYLVAPATTKEADLFQLSASLGVFNLDGVIITKLDETYHF
                     GGLYNFARKSSVPLSYFTNGRETARHLETADPQRLVSALFAKEWNK"
     gene            14157..14516
                     /locus_tag="PL000_05490"
     CDS             14157..14516
                     /locus_tag="PL000_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88380.1"
                     /translation="MADNEISLERQDPAEEEKKKADSSKQAAANAPAPVLVEFTITVS
                     VIFLVLVFFTVSGVLLVKGSTLLDFVFRTSVSLVVIGGLLAIIARQISSATTSQRKDA
                     QPEETSGLDPSSPSEVK"
     gene            14521..15273
                     /locus_tag="PL000_05495"
     CDS             14521..15273
                     /locus_tag="PL000_05495"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNC88381.1"
                     /translation="MVMQMEATGILEEFIANRSPELREKLVLQSVPLVHYLLGRMGVT
                     RERGSDYEDLAHQGLIGLIEAVDHFDPKFNTKFSTYASLRIRGKIMDYLRTTDWMPRA
                     ARKRSRLIQKSITTLWSENFREPTEDEIASYLGTNVEDVQQGLADTNRVLVSLDMMME
                     GDHEGEVPLHEKYGDEKQANPLDLLEEKDMAEDMAEAIKSLDEREQLVLSLYYNEELT
                     FKEIGKVLDITESRVCQLHARALVSLKAMMES"
     gene            15270..15662
                     /locus_tag="PL000_05500"
     CDS             15270..15662
                     /locus_tag="PL000_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88382.1"
                     /translation="MSNISKVSLEDSLYVLQLARETALAQNRQAQAKRMAPLVEEMRG
                     LVANPPQAAASPSTGVMGQSDFKALLNISQEKTSQNQTVDSVSSVLERNRLIGAMSEA
                     NMSDVEIARQFSMSREEVQLVLSFQQRK"
     gene            15678..16436
                     /locus_tag="PL000_05505"
     CDS             15678..16436
                     /locus_tag="PL000_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNC88383.1"
                     /translation="MIKGLYSAVSAMVMNANRQQVISHNIANLQTPGFKQILTTAQDF
                     MPEQAVYTNGNLQTSPIEFVGTLGLGTQVGQEFIDFGAGAMQNTNNPLDFALQGSGFF
                     TVKTPDGNRYTRDGRFLRDAENTLVTVDGYQVLDDAGQAIELPDGEVSVSPEGILSVN
                     GTEVATLGIGVFADPEAELEHTEGNLFTGPAASTGEEVPQVVQGYLETSNANPSQLMT
                     QLVEVGRSYEAAQKLVQNQDELLGKTIASLGRIG"
     gene            16449..17192
                     /locus_tag="PL000_05510"
     CDS             16449..17192
                     /locus_tag="PL000_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNC88384.1"
                     /translation="MPSSLFHTLNISRQDILSHMLDLDVTSSNLANVNTAGYKATRSN
                     FQEMLNAQLKEGTQMRSSQLLTMQGSLRATSNPLDWAIQGDGFFSVTLPDGTIGYTRD
                     GQFSLDAERTLVNANGYPLVWDGEIAEGMTNISISSTGVVTATDADGATVEVGTVQLT
                     RFPNPSGLTDNGNNILLESEVSGAAQTGDPGAENFGTLSGFKVENSNTDLAQELTSLT
                     ILQRNLSMSLKAFQQTDTMISEAINLRKA"
     gene            17258..17554
                     /gene="flgM"
                     /locus_tag="PL000_05515"
     CDS             17258..17554
                     /gene="flgM"
                     /locus_tag="PL000_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNC88385.1"
                     /translation="MKIEPTGIQPLSTKATENANPIEKRDEAGEVESTRGGQDKVEMS
                     ENARLLAKARAALGNTEEVESERLAMLKQQVQSGDYTVQVGELARKLLAKLFPK"
     gene            17578..18099
                     /gene="flgN"
                     /locus_tag="PL000_05520"
     CDS             17578..18099
                     /gene="flgN"
                     /locus_tag="PL000_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNC88386.1"
                     /translation="MVNEEELTKQRYELEQVLVLLFRALQDLLALSKKERISLLNEPN
                     QILQIVEDKEALLDKISMLEDKCRQVVQALSLALDVQLAETSIQALLPFLKPEDAARI
                     KNLTDGIYSLTSQARELNHASQALALTKLDWLKATQSFLIDIFQPEAGYRSPKDASKR
                     QEPAAGLGVERRA"
     gene            18109..19509
                     /gene="flgK"
                     /locus_tag="PL000_05525"
     CDS             18109..19509
                     /gene="flgK"
                     /locus_tag="PL000_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNC88387.1"
                     /translation="MPTISSGISIALQAVLTHSQVLEITEHNIANASTPGYRRQSAVL
                     TAETPSSINGAEYYTGAGQRGAGVTIDRIQRFNISYFDGRYRAVSSEAGNWETQSGIL
                     SQLESVLAETSADGLLPKLDQFWAGWQSLSNDPTNTSLRTVLLDNAASLATAFNRRAT
                     QIMQLRSDQNLTVSSQVEEINTLAGDIARLNENISRVISVGEQPNDLLDKRDVALDRL
                     AKLTGAVSTEQKNGEVLVSVGGHVLVVGHDAFTLETRANPSDTSILDVYWSDNQQLTP
                     PSGELKGLLEVRDRVLVDQLNGLNTLAGGLISQVNTIHNSGYGLNNATGLNFFSGSDA
                     LSISVNPAVDAASIATSSAANQAGNNDIALQIAGLKTVKGMKAGTATLNEFYNSQITE
                     LAVLTKRATDHSYQHGLVTDALGNQRESMAGVSLDEEAANMAKAQKAYQAAARVLTAY
                     DDLLDLVINRMGRVGL"
     gene            19525..20481
                     /locus_tag="PL000_05530"
     CDS             19525..20481
                     /locus_tag="PL000_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88388.1"
                     /translation="MRITHQMMTDGAIQNMADNLEKVSKLQNRIASGKNFQVASEDPT
                     HASASLSLRSHLQTLESYTDTAELTQNWMTATDNAFDQLEQLAVRANNLILRGLNDSL
                     SGRERAESLATEMQTLINQAVELGNTSVNGQYIFSGYQVNQKAFALSDAAATLPDYQG
                     NPFTPKVITYLGDQGNIQRSLGPDQSVTLNVRGDQAILGFLQNMILASNALQQNNIQD
                     TGDPVTDPLTLESTLSALKSSLETLDQYRTSNGARMRQVDSAANFLETVKLETRSLLS
                     KNEDTNMAEGIALLANQQTTYEAVLEVSQRAISALSLFDYLR"
     gene            20507..20800
                     /gene="csrA"
                     /locus_tag="PL000_05535"
     CDS             20507..20800
                     /gene="csrA"
                     /locus_tag="PL000_05535"
                     /inference="COORDINATES: protein motif:HMM:NF002469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNC88389.1"
                     /translation="MLVISRKINESITIGDNITVTVLDIEGDRIKIGIDAPRELIILR
                     QEIFQAVQDQVKIQELLAEESKPESLDQLRKLLVSETEEALAEPAQPPTPPSK"
     gene            20924..22927
                     /locus_tag="PL000_05540"
     CDS             20924..22927
                     /locus_tag="PL000_05540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNC88390.1"
                     /translation="MEIIFDINEEELPIFLAEVDEHLQALDDSLLRLEHGEADPELVQ
                     TVFRSAHTIKGMSGMIGHQRMTDLTHAMETVLDGVRKNDIQITSKLINVCLEAVDHLR
                     LLRDEVCDSQVCDVDMKEIVFALKGLIGEETPRQEARFTPEPETGADLPDGIFHIQAV
                     IDVNSMASAARAFQLMMALQDAGEIQTMTPSQAEIESAASVREFSATVASSQSFEQVS
                     AALTRLSGIEEISVNGRLVLSGGKGILQQEQASVKVGGDRSKAGGVERRSHNPFGRRS
                     TDMAIRMNVERLDNLVNLVGELITDRNHLRQIHSRMTRMNSNYDIITETITHLGRITD
                     ELQEEVMHIRMLPIASVFGKFPRMVHDMSLKVGKQIDIAIHGEKTEMDRSMLEEINDP
                     LIHLVRNSVDHGIETPEERIAAGKPARGKITLTARHEQGRIILTVEDDGGGINTDKLR
                     KSAVQKGLISEEEAASLNEEQALDLMFMAGLSTASKVTDISGRGVGLDIVKTNIQRIN
                     GSMQVETNPGQGTRFHVFLPLTLAIVPALLVRVQHSTFAIPLVMITETFRLDESEIKH
                     VYRKPVTMLRESVLSLIDLSEIFNLPRSREQKKSMYAVVVQWGRQRVGVIVDSLLGEE
                     DIVVKPLGSFIGDIPGISSAAILGEGQVALIVDVFGLFKLAGI"
     gene            22937..23554
                     /locus_tag="PL000_05545"
     CDS             22937..23554
                     /locus_tag="PL000_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNC88391.1"
                     /translation="MQETAQFDEKLLEMLNTIASEGIKNAADGFSGMVGHKIEVSDPV
                     LELVPLLTIPKVVGDPENDAVGVYLRFEGDMVGQIMMIIPHHKALELADLLMGVPQGS
                     TQHLGTLERSALGELGNLCGSFFLNSVAKTVGASFRPSPPAIMVDMVGAILDIVVATV
                     GGVNEHVLLVHANFMDGTRSVETDFWVIPDMKALQNLMTAKKTDV"
     gene            23551..24048
                     /locus_tag="PL000_05550"
     CDS             23551..24048
                     /locus_tag="PL000_05550"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNC88392.1"
                     /translation="MSDPISLGLGELAASHNPEDVLIAYGLGSCVGIAMTDPLAHVAG
                     LLHAVLPESANGTENDEASTAGKYVDTGIANLIEAMIKAGANKSRLIVRMVGGANMLV
                     APGMTSTFDIGTRNVEKARTTLQRLNMKITAEEVGGHTGRTLRVYVADGRVTVRVIGQ
                     KEHEI"
     gene            24104..24460
                     /locus_tag="PL000_05555"
     CDS             24104..24460
                     /locus_tag="PL000_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88393.1"
                     /translation="MSKILIVDDAEFLRVRLTKMLSTDGYEVFQAENGLKAVNSYKEL
                     HPDIVLMDVTMPEMDGLTALKEIVGFDPKARVIMLTALGQESVVLEAVKSGAKDFVVK
                     PFEQDRVMKAITKLLA"
     gene            24467..25564
                     /locus_tag="PL000_05560"
     CDS             24467..25564
                     /locus_tag="PL000_05560"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNC88394.1"
                     /translation="MTDNGISPKSLRVMVVDDSAFMRFSIIQQLNEDPEIQVVGSASN
                     GREALRLIPQLQPDVITLDVEMPHLDGLSTLREIMKDFPCPVIMVSSLTKEGASETIQ
                     ALMLGAVDFIAKPDNRLDIRIAMTEIITKIKLAAKARVKQTLPNLLKIAPSTAASQPE
                     KAIRPYSRQAPVILIGSSTGGPGALSEILPMLPSSLPSPIVIVQHMPVGFTRSLSERL
                     NTISQLKVKEAEPGDSLLAGQVLVAPGGFHMTFDEDERVALNQKPTVHGVRPAVDVTL
                     NSMNRHFGKNMVGVILTGMGSDGAIGMSILHSAGGYVIAEHESTCVVWGMPRSVVEAG
                     AADAVLPRPQIAKAIESVVREMSKQDAKHGT"
     gene            25554..26342
                     /locus_tag="PL000_05565"
     CDS             25554..26342
                     /locus_tag="PL000_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNC88395.1"
                     /translation="MEPEIYTQIKTSIKQALNIDLESYKEEQMKRRLDSWLVRTRINT
                     WKEYFSLLKNDASELEKFRNYLTINVTEFFRDPNRWESVRTEVLPHLLKTCNQASGLK
                     LWSAGCSTGAEAYTLAILMEEAAPHQRYSILATDLDRGALLKARSRGPYSVEDVRNLS
                     SAQKERHITPAAPYFANEKLQRNIRFQEQNLLEDPFEGNFDLIVCRNVVIYFTTATKD
                     LLYAKFCKALRPGGVLFVGGTEIISGAAKFGFQNFGISFYKKME"
     gene            26413..26964
                     /locus_tag="PL000_05570"
     CDS             26413..26964
                     /locus_tag="PL000_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNC88396.1"
                     /translation="MKFLNPFVQAAVEVLKTEVGANAVRGEVSLHKSSLTSDDITVLI
                     NLIGDVYGVVMYGMSTVTCLNMVSKIMGQEFTEFNTLAQSGVAELGNVISGQATIRFS
                     ESGYKSNISTPTVLNGNGVEISTLDFPRIVVPLETQFGMLTVHLALREKKNGEAHLVE
                     SFISSIKAPVPQSDSQVPPAATG"
     gene            26978..27502
                     /locus_tag="PL000_05575"
     CDS             26978..27502
                     /locus_tag="PL000_05575"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNC88397.1"
                     /translation="MNVKFLNPFVSSAHDILRMELHETVQRGELRLENGSYHTDDATV
                     IISLIGAVDGTVFYGMSKEAAIQFASALMGEKFEALNELAQSGIAELGNVITGQASMR
                     LAEAGFESNISTPSLLIGKGASISTLEYPRLVVPLMTSIGSLTIHLSLRENPQANLKT
                     PQLAVPAAFIPPRP"
     gene            complement(27508..27903)
                     /locus_tag="PL000_05580"
     CDS             complement(27508..27903)
                     /locus_tag="PL000_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88398.1"
                     /translation="MFTNLSDGTKTALQVVFVLALVGAAAAFLISMGLFQTSAESHLV
                     SFEVNASGGYALITLQAGSESIEKPATVTVPWSKTIRIKSGTEVYLTASNPSQTGELA
                     CRILLDKADWKMETVNAPKNGVACAGIVP"
     gene            28235..29806
                     /locus_tag="PL000_05585"
     CDS             28235..29806
                     /locus_tag="PL000_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNC88399.1"
                     /translation="MATVDITRIASNIGALNSLNSLMNINAKLATHQTRLSTGRRINS
                     AADDPAGLTIATKMLARSEGLKVTLDNIGDAKNMLSVAEGGLSKMTDIITQMRNKAEQ
                     AASDTLGSSERATIQTQLSSYAQQLQDIIDQTKWNGVKLLDNSTGTKLFQTGVDEGES
                     TTWTLPNALDPATLSLSKVVATAQATTSVLGASFGANAAQSGTLNNMSELSTGDYSFE
                     VLDKATDATHGNFNLDTSSKLVTGLSGVTGSASPASADKMTSGHYKLTIDAATSATST
                     SYTLTNLDDSTWGTNGSIAVSGVDLSATGSSGALTDGTGTNAAAQLGVTLAFTGDAAD
                     LVTGQSMTFEFIAMNEVKLELNDSSGQAMQINRVAADGTTNYSASSAYVAGAGTYQSG
                     RGASATLAAFGTVSSTVGNKLAFTYEQANNFSVNVSNAAKAGAYLTTTNNALDKVTSA
                     LSDLGSLMSRLTFKEESISTAQVNVEAGYNRIMNANMAEEQMNASKYTILQQTAIAML
                     AQANQAPQNLLSLFR"
     gene            29901..31616
                     /gene="fliD"
                     /locus_tag="PL000_05590"
     CDS             29901..31616
                     /gene="fliD"
                     /locus_tag="PL000_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNC88400.1"
                     /translation="MATTITSIDASIRNLTPSFQTAIKTTIEAESAPLKRVQTLKDSM
                     EVRRGVYTDVKSNFDALQSAIQALISTQATYGLSTVAKASVTPAISGSTVLTVSKTSE
                     TAPVTDYDFTVTKLAKAQSRATVAVANPDLALNKSGTFWMGGTGSAALQTETAPGEYG
                     DFVASTSLTAATVSTIASGQKELGSGDYTLQVRDSSGVRQFRLVNADGNAVSIRQTDG
                     STNYTADWQTMTDGAFDTGRGQTLTLDKSGDLESTTFHYTARGVSITINSSDTQRNIL
                     NAINAANQPEGHDFKASIVASQLVITANQTGTNHTMLYTDGAGLGFNSLLQDAQNAQF
                     TVNGMSVSRASNNNLTDVVEGLTLSLAGDAEGKSAQLSINANTDKAAGLMNAMVSKFN
                     TTITHLKDKMASTAKTENGKTTYTRGALSGETVFSGFRSEMLYRMSRSYTNSGSFKRF
                     EEVGLTFDKDMKLTFDSAKFGEALKNNASDVTALLDAGMGEINTTLSRYTGTSGILTK
                     TLTTLESQGKEYDQRIARYNTFLETRKQTLYKQYMEYQTQLVDLGNTAIMFGINLGSS
                     VDTSG"
     gene            31691..32026
                     /locus_tag="PL000_05595"
     CDS             31691..32026
                     /locus_tag="PL000_05595"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNC88401.1"
                     /translation="MNQSSIKPISGIQSSDGSALTKALNTAQLEMTALQPRPATTARQ
                     EKTQAETRQESTELGSLANVSIHFRVDDKTSNVTVFLVDRKSKKVLRSIPASELQKLQ
                     IGDLLKLTV"
     gene            32155..32526
                     /locus_tag="PL000_05600"
     CDS             32155..32526
                     /locus_tag="PL000_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HNC88402.1"
                     /translation="MYQSAYRNQYRQQDVMSASPLRLVIMTYDLAIRSCEQQDFGKAV
                     KTISALRDALDLDYPEVAAGLFRLYQWCLDCIRKGDYTSAITTLTELRGAWVATEEKI
                     AARQTTTTVQTYSTSFGNILT"
     gene            32694..33140
                     /gene="flgC"
                     /locus_tag="PL000_05605"
     CDS             32694..33140
                     /gene="flgC"
                     /locus_tag="PL000_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNC88403.1"
                     /translation="MSFWTSLRIGLSGLTAQRLRLDLISNNIANAQTTRTEKGGAYQR
                     QDVIFMPEEQNDFLPKLVAARRGQEGSLQGGVRVAEVTTDTEAGPRVYDPTHPDADAD
                     GYVTYPNVDVVVEMTNMLSATRSYEANLASVEASKRMALKALEIGR"
     gene            33146..33451
                     /gene="fliE"
                     /locus_tag="PL000_05610"
     CDS             33146..33451
                     /gene="fliE"
                     /locus_tag="PL000_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNC88404.1"
                     /translation="MALPPINSRPIPAIPEINSTQKPSASSNTQQITQSFEDMLTSLN
                     QSQQNSDNLMEQLARGEDVDLHTVMIGLEENNVNFNVALGIRDKLVEAYREVMRMQV"
     gene            33472..35148
                     /gene="fliF"
                     /locus_tag="PL000_05615"
     CDS             33472..35148
                     /gene="fliF"
                     /locus_tag="PL000_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNC88405.1"
                     /translation="MLTTVQQQFMTVWKKQSTGKQITIVALILAMLILTPVLVNWANT
                     PSYSVAYTGLSEADAAQIVQKLDENNIAYQLKSSGTIEVPSEQVYTTRLLMAREGLPQ
                     SSTVGYELFSGSSMLGMTEFSQKVNYQRAIEGELERTIGSIEAVKAVRVHLVIPEKSL
                     LTSEQSLTTASVTIQVNPGHLLDASQVRAITHLVASSVEGLKPESVVVVDSDGNLLAS
                     GTDSETELNLTIKDDQRAIEMAYATEVQKRVQSILDKILGPNRSVVQATVEMDWTQRE
                     ITSNTFSPTESAIRSSQTINESSTSGDTTGGVPGAASNLPTPVATAAGTGSSTYQRSE
                     ETLNYEVSQVQAHEVTAPGEIKRISVSVMVDGITDQAQMDIIRSAVQVAAGIDSTRGD
                     QIAVESFDFDRTALDELSAELEKQQQQELYMQIGMAVAAAIVVLALLFFILRLINNTR
                     NASKDSWKAVMRPVGEVAALTDTHYKEAALKLQDDLSTALSRLAEKEKTNTPRVETNE
                     DVAVQLRSRNQSAKAIEDEQNARIISRLTEENPATVAEIIQIWLNEGKKS"
     gene            35145..36146
                     /gene="fliG"
                     /locus_tag="PL000_05620"
     CDS             35145..36146
                     /gene="fliG"
                     /locus_tag="PL000_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNC88406.1"
                     /translation="MTTGIEKASALLLGLGVDRSSRVLQYLNEGEIEQVLMVLGKPGT
                     VAPEVRHEALREACELSLSDTAEMRGGIEYTRQLLARAVGPRRGTEILERISVHQQLS
                     SFEMLKNANTTEVATLLQEEMPQTIALVLSYLEAKVAADILTSMPKEIQIEVTLRLAA
                     MDRVSPQVVQQVEKSLKSKLSSVINEADFRATGGVTFLVKVLNQVDRGAQKSIFDALE
                     TTNAKLVEEIRANMFTFDDVIKLDDRAMQRVLRDINKSDLALALKGAPDRLREKVFAN
                     LSERARENLKEEIDILGPQLAKNVYTAQRKIVDVCRSLEESGEIVIAGGGGENEVIA"
     gene            36130..37086
                     /locus_tag="PL000_05625"
     CDS             36130..37086
                     /locus_tag="PL000_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNC88407.1"
                     /translation="MKSLHKAKPNSALEAWSPPELDADASVNAQANRKEQVLAIFQKE
                     EEGKASSQENSILRGGTAGKNLTTWQPGLLDDHNASTRADNWSFIEVTDTPFDRSWKV
                     QSPGVFVKETSQRDMFFSEKEIATALERARLQAEEIILSAQAEADDLLLQAQSEIDHQ
                     KKEGYLQGKNEARAEIETAAQAIQTMVTEIETWKAEFLSQGEQILLEMLKDMARKMFG
                     EGAKLDAPALHANLNRIIGNAHGLGMLKIFLNPIDARQLDPFWSEQQMLILGEQVKIV
                     PSNNILPGGCLIKGNMGTVDARVETQLDSILNTFDDPQATAE"
     gene            37090..38421
                     /locus_tag="PL000_05630"
     CDS             37090..38421
                     /locus_tag="PL000_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018035956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNC88408.1"
                     /translation="MQNTNLNLARFRNTLSQVNPLAITGRVVQVVGLTVEIMGLNCQI
                     GEICEIQTSNKTSLMAEAIGFRNNRMLLMPLGSMEGIQPDSTVRSVSQVFKAPVGLSL
                     VGRVLNGLGEPIDGKGPLKINNWASINQSPPHPLQRAAIEEPLVTGVRVIDGMLTCGK
                     GQRMGIFAGSGVGKSTLLGSIARNSESDISVIALIGERGREVREFLERDLGPEGLARS
                     VIIVSTSDQPALMRLKAASVAMTIAEYFRDEGMDVMFMMDSVTRFAMAQREIGLSIGE
                     PPASKGYTPSVFALLPRLLERAGKGETGSITGFFTVLVEGDDFNEPICDAVRGILDGH
                     IILSRTLAARNHYPAIDVLNSVSRVMPAITTKEHLQLAAFARKNLAVFEKFRDLVNIG
                     AYVKGSDPEADTALLVMPDINRFLQQNRSEVSPFQDTLQTLNEISQTAPAS"
     gene            38439..38888
                     /locus_tag="PL000_05635"
     CDS             38439..38888
                     /locus_tag="PL000_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88409.1"
                     /translation="MAPKFSLQNVLDLRHGKVELLEIELGKLTTIHRETQTLHASLQQ
                     YQASLLNQLNDAQTGELDMVKLNLLRLNILQLNRHIELVVLELKKQTMEIDAKRAELV
                     KAKQSEETLEILKRKRNEVYMAEQVQLEAQAQDDIYIARAFRNQRGA"
     gene            38895..39476
                     /locus_tag="PL000_05640"
     CDS             38895..39476
                     /locus_tag="PL000_05640"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC88410.1"
                     /translation="MDNSFLTGRIQSVMQNLMLTMINKLLTRLESKSQANGTTGTKST
                     AQTTSTSLASTSSAGQAASGNFASLINQASQKYDVDPNLIQAVIKAESNFNPNAVSSV
                     GAQGLMQLMPGTARGLGVTNSLDPAQNIDGGTRFLSQLLDRYNGNVRLAVAAYNAGPG
                     AVDKYNGIPPYQETQTYVTRVLGYFNSSNEWQA"
     gene            39478..39876
                     /gene="flgB"
                     /locus_tag="PL000_05645"
     CDS             39478..39876
                     /gene="flgB"
                     /locus_tag="PL000_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNC88411.1"
                     /translation="MSDSLFSDISTRTAKAALDGLSLRQQAISRNIANIDTPGYQAQT
                     VDFQETLTRLVENDDLLPMSTTHEAHLTTAASNLTLSTSLRPGGTQRADQNNVDIDVE
                     MADMSEAGIQYQAISQAISKKLLLLKTLAK"
     gene            39876..>40426
                     /locus_tag="PL000_05650"
     CDS             39876..>40426
                     /locus_tag="PL000_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88412.1"
                     /translation="MMPLGRQPTVTPLSSDGLPAQPQLRDSGAAQAPSFKEVLKVEEK
                     KLQQTTNTSNAAALLATLQLPAIPAIEITQLVGTNKPTDPSALTIDSIQTETASPKQD
                     SPLSNPQNGTNTNQSKPAYTPPISSIPTTTKAEQTPDAPTETPAAKITTKPTNTAKAD
                     TVDIEAVKVKSQPTPVVTKESPKA"
BASE COUNT        10385 a        11620 c        10004 g         8417 t
ORIGIN      
        1 ccgaatcaga ccgctatctt ttcaaattcg cagatggggc aacattcacc atccttgata
       61 aatggaccag caaaagcaca accgtgtggg gcgacccgca tgtggatgtt gatgatgtgc
      121 aggggaacag caatggagat ttcaaagacc tcaaggcaag cgatgaattc accacattca
      181 tgctaagcga tggaacacga gtgactttca aggccaggga tgcgggcatt atcgagcagg
      241 tggatatttt caaaggatct caacatgtaa gaggaattgg gcaggcggct gaggggtatt
      301 cacccgagag cggtcttttc cttcaaaccg tattgcagga tggaaaaacc gcagcgatct
      361 ccaccccgtc tggagatacc gtcctctcag gcggcgatgg aaatgactgg tacgacagcc
      421 gcggcaattt ggtgtggggc aaaaccacag ggcctgtggt cacccagcgc cccaacgcca
      481 cgttggaatt tatgtaccgc cagaccgttt cacaatcggt ttctgtaaaa acgatctcaa
      541 aagacgccta gcacaagccg atcaacacct gttttcaaga ggggaaattc ttcaggctaa
      601 atatttttag ccaatcttta tattcctttc acggcctatt tacaggagat atggggacgc
      661 tatgctaaat ttatgccgaa aaagcctccc aaccgaaaag gacgataccc gtgatttttc
      721 taacccgcac caatggagcc atcttttaca tcaaccctga attaattcag acggtggaag
      781 caactccgga tacggttgtc accctggtga acaataaaaa attgatcgtt aaagatactc
      841 cacgggaaat cgccgagcgt ttcatcgaat atcgaaggaa gactttggcc ccttttgacg
      901 ccccaacttc aggcaggtag gaagcgatcc tttttcgaag gcaaaaagaa acaaacttag
      961 gagcagcata tggatattgc gaccatttcg ggtctggttt tggccctcac agcgatcatc
     1021 accgccttga ttctggatgg tggttcccct gcggaattgt tcgcagcccc ggcagcgatc
     1081 atcctcatca tcggcggatc catcggcgcc acggtcgtta cttccccgtt gaagatcgcg
     1141 ttgaaactgc cgcaattcat catgcaggca ttcaagacga ataagttcaa ttcaaaaggc
     1201 gatatcgagc ttttgacgaa actggccgac aaggcacgcc gcgaaggatt gctcgccctc
     1261 gaagaagaca gccgcaaact taccgataaa ttcctcaaaa aaggactcat gatggtggtg
     1321 gatggcgcgg aaccggaaca ggtctctgcc atcatggaaa acaccattga ccagatgcgt
     1381 acccgccaca aacaaggcat tggcttcttt acagcggcgg gcgggttcgc ccccaccttc
     1441 ggaatcatcg gcaccgtgat ggggctcatc agcgtattga aacaattgga taatccatcg
     1501 gcattgggtg aggcgatcgc ttctgcgttc cttgccacac tgtggggtct gttgaccgcc
     1561 aatctggttt atctgcctgt ggctggaaaa ctcaaagcca aaagcgaaga ggaagcccac
     1621 aaccgctaca tgcagttgga aggcatcctt tccattcaag cgggagaaaa cccgcgcatt
     1681 gtccgcgata agttaatggc ctacctggct ccatcagaag cacagggtga aaaagaaggt
     1741 gacaaggcca aaggtgccga caagaaagca cctgcagcga acaccgggaa ggcacaagca
     1801 tagccatggc acaccgcaag gaagaccacg aagagcatca cgacgaacgc tggctcgtca
     1861 gttacgcaga tttcatcacc ctgctgatgg tgttgttcgt tgtgctgtac tccatggggc
     1921 aggtcaatgt ggagaaatac aagcagctgg ccgaaagcat gcgcacggcc ttcacccttg
     1981 gcggcgcatc acaggtcgtg gacagccaga tcaatcaagc gggcggcaca agcgaagatg
     2041 gttcatccaa accgatcgtg gtaccgggca ttcctgaagg tcccacccaa tcggaagaag
     2101 ttgcaggtca attgacttcc atgctgtctt cgcagaacct gggcaaccag gtcagcgtcc
     2161 agaccaatat tgaaggtgta ttgatctcat taagtgagcg gttgatcttc aaaaacgacc
     2221 aaacggaatt acccgcagaa tcactgcaag tgctcgatac catcgtggaa atgctgcgcc
     2281 cgatcgacaa taaagtgagg ctggtcggcc acacgaacaa cacgccgccg acgaaccccg
     2341 cctacccggg caactgggag ttgtcgctgg cgcgggccac ctcagtggca aagtatctga
     2401 tcaactcagg catctcgccc gaaaggctga tcatttccgg gcagggtgaa tatgcgccga
     2461 tcttcccaaa cgacaccgaa caacacaagg aattgaatgc acgcgtggag atcatcatca
     2521 tttacaaggt tgacagcaac atcatagaca tcacctcacc tgtcatcatt ccataaccca
     2581 ggcaacttct gaagcggaga cctatgttag acaaaatcct aaaaatcctc aatgtgatca
     2641 cccaggtcat tctggtcatt acggcgatca tctcgctcat cacagcttac gtgatctttg
     2701 ccccagatga actccccaag cccttccgcc ttgtctacga ctatcaaact actttaacca
     2761 ccccgctggc acccgccaca gaagccgcag tggaagccac cgaagccccg caggaaaccc
     2821 acgaattcac cccgggcgaa ggcatcatgc tcaatatgtc caccaagatc atcaatctgc
     2881 tggaccccgg cggacgcaaa tacatccgtg tgaccattgt ggtcgaattt gcccccgaca
     2941 acccggaata cgaatccctg ccagaagagg aaaaaacagc ctacctgacc gcatttgaag
     3001 aaaagctcaa ctcaaggctg cccatcatgg atgacaccgt catcaccctg ctttccacca
     3061 aaacatacga agacctctac accgccgaag gcaaggaaaa attgcgcatg gaaattcaac
     3121 agaccctttc tgaaaaacta accgaccttc atatcatctc gatttacttt accgaatttg
     3181 tggtccagta accatgttag ctcaatctga aattgatgca ttactctccg gcgccattga
     3241 cttcgaccag aagaacggcg gcgataacgt caacctgacg gataaggtga atccgtccgc
     3301 tgaatcattg ggaccggatg ggaaacgtga gaaaaaaatc cagccctata acttctggtc
     3361 acctgccaga ttttcgaagg agcaaatgcg tgccgtggaa ttggtacatg aagacctgag
     3421 cgaacgcctc accacctcac tgcccacctt tctgcgcacc aacgtccgcc cgcgtctcgt
     3481 acacatcgag cagggacgct tccacgactt catcaaggac tttccgcaaa acaccctctt
     3541 ccatttgatt tcactggccc ccctgcccgg tcacatggtg ctgaccatga gtctcaacgt
     3601 cagctatttg atccttgagc agcgcctcgg cggcaaaatt gaaggccgct ttaccgaacg
     3661 cgccctgacc gagatcgacc aatccctgct ccgcggattg gtggaacaca tgctcggcga
     3721 tatgaaaggt gcttggagca aggtagtctc gcttgaaccc tcactcgaag acagcactgt
     3781 gaatcaacac tgggtacaaa tgatgatagg caatgaacgg gtattgctgt taaccatcga
     3841 gatcaacatt caaggtgtca ccggcacaat gagcgttttc atcccattca acaccttgaa
     3901 acccatcacc gagatcctca accctcacat ttggatcgca ggacgcaagg aacatcagca
     3961 ggaccctgta gcccgccaga tggcaatcca agccaccatg aaagccattg tcccggtcac
     4021 tgttatttta ggagccgtag acctgcctct gaaagaattg attcaccttg ccccgggcga
     4081 tgtcattgaa ttgaacacca acattgacag tccgctggtg gtgcaggttg ccaacaaaaa
     4141 acagttctat gcccgcgttg gaaaatcccg caagcgtttg ggcgtgcaga tcacccgcgt
     4201 ttaccgtgaa caagacgaag cccagatggg attttgataa ggaaaactaa aatgaacaac
     4261 atcaacactg aaaccgaaga aatgcccgtc aacaaaactg ctgaaccgat cgctgaaagg
     4321 ccagccgcca aatccgattc cgtgaacgaa tcgaatatcg aactgctgct ggatgtctcc
     4381 atgcgtgtga ccgttgagtt gggacgcacc cgcatgaaac tcgcccagat ccttgaactg
     4441 cagcacggct ccgtcgtcga attggatcgg ctcgctggcg acccggtgga catctttgtc
     4501 aacgaccgca tggtggcaca cggtgaagtc gttgtggtgg atgacaaatt cggcgtccgc
     4561 atcaccaaca tggtttcagt caattcagaa aaggcatcct tctaatgacc gacctccctg
     4621 ccaaaattag acagtggatc gcgaccagca gcccgaaaca aaagctgctc gcctctctcg
     4681 cgaccctcag cctgcttgcc accgctgcgc ttatcaacat gggcggcgcc tccagcgttt
     4741 cgcaagaccc gcttgattcc accccctttt attttctcag tgccttcgta aaactgatcg
     4801 tggttttgct gctcattctg ggcagtgctg tgatcttcaa acgctggtca caagccaacc
     4861 cgttgacgaa gaagacccgt caactgcatc tggtggagag cattcggttg tcaccgaagc
     4921 aggcccttca cctggtcatg atcggcgacc aaaaactgct gatcggcgcc accgaccaaa
     4981 gtatcgcact cattgccccg attgaaggcg atccttccct gcctgagcag gaaacccaac
     5041 ctgccgccag tttggatttc ggctctgtca tgcggtcttt caacattccc accttgaatg
     5101 cacattcaaa agaataaaac cagccatgct caacacatcc cttcctctca tcacatcacc
     5161 agagcaaaag aaatctcacc gcccgcttaa aaggcctgtg ctgttgatcg tccttgcgtt
     5221 ttcagtggtc ctgcttgcca gcggatgctc cgccgtgcct ggtgtctcac tcaccgtgga
     5281 tggacaatct cccgacccca aacaggtgac ttccggtgtg caactgctcg tgctgatgac
     5341 cgtgctgtca ctggcgccga cggttctgat ccttggcact tcatttaccc gcatcgtcat
     5401 cgtgctttca atggtgcgca atgccatcgg cacaccgtcc attccgccga accaggtcat
     5461 cattgggttg tcacttttgc tgaccttttt catcatggcg cctgtgtaca aacaaatgga
     5521 tgaacaggcg atccagcctt acatcaacca acagatcgac cagaaaactg cgctgaaact
     5581 ggcccaagcc ccgatccgcg acttcatgtt caagcagacc cgcgaaaaag acattcaact
     5641 cttcattgat atgggaaatg aaacccaacc cgccacactg gatgatatcc cctccgtttc
     5701 cctcttccca gcctttgtca tcagcgaact cagaacagcc ttcacaatgg gctttgtgat
     5761 ctacattcca tttttggtga tcgatctggt tgtttcaagc attttgcttt cattgggcat
     5821 gatgatgctg cccccatccc tggtttccct gccgttcaaa ctgctcctgt tcgtcatggt
     5881 ggatggctgg tatctgctgg ccagcagcct ggttttgagt tttgctggcg gttcatgagg
     5941 taaacaatga ctgaagccta cgtactaacc ctcggacaaa atgcaattgt catggcgttg
     6001 attctggcag gacccatttt gctggtcagc ctgttgatcg gtagtctggt aagcctggtg
     6061 caggcagcga cccagatcaa cgaatccacc ttaatcttca ttcccaagat catcggcatc
     6121 attctcgtgc tggtactgct cgggtcatgg atgctgcaac actccatcac gttcacacac
     6181 accctgttca ccagcctgcc cgagttggtt cactaggaag ccatcagaaa gcaccgatat
     6241 gccagctatc agcgttgaag atatcatccg ttccattgag ttgctcaagc ccatgccgac
     6301 cagcgtctcg catattttgc gggaggttga caggcaggat gtgacgatcg attctctggt
     6361 gggtatgatc gggctcgatc aggtattgac cgcgctggtc ctgcaaattt ccaattcggc
     6421 ctcactcgga tactcccgca cttgttcgaa tctgcatgat gcggtcatgc acatcggcct
     6481 gcgtcgactc aaatcgatcc tgctgacatc ttctgcaacc agcatgatgg gacggagttt
     6541 gaaaggctac cgcctgggtg cgggggaatt atggcgccat gcactggtgg tcggtatggc
     6601 ttctgaatgg ctggcccagg tctttcatta tccagacccc gaacaggcat atgtggcagg
     6661 cttgattcat gatatcggca aactattgct cgatcaggcg gtgctatcca attactccag
     6721 aattgccgat tatgtggaga aatatcaaat gccgttatgg caggtggaag aaaagctgat
     6781 cggcatcgac cacgcccggg tcggcagttt gatcgccgag cattggaatt tccccgtgat
     6841 ccttgtggat gcgatccgct tccaccatgc gccctcgttt gcgcggggca accagcgcct
     6901 gcctgccatc gtcaatctgg caaacgcttt cgctgtagat tatcaactgg aaaattcagt
     6961 cattcttgat tcacagatcc accctgaatc tttgaatatt ctcaaactga ctccccacaa
     7021 actggaagaa ctcaaagtca gcatgaaggc ttcgggggta ttcccaacaa ggtgacaaaa
     7081 tgaaacgcga aagagcatcc attctcatcc tcgaaccgaa cgcactgcaa tgtgacctga
     7141 tcaaactggc actcacccgt caccagatga ccccgatcat atgtgcccag gccgatcatt
     7201 tgcgccagca attggcgcag catcttcccg atgccttgtt gatcgatacc caattaccgg
     7261 gagtaaacgg tttggatttg atccagcgcc tgaattcaga ggtgcttttg aacaggacga
     7321 agatcttctt cctttcgtcg ctggcttttc ctgaagtggt gcaaaaagcg gccagaatgg
     7381 gcgccagcgg cttcctcgtc aaacccctgg atccagatct tttggcggcg cgtattcaac
     7441 aaagcctcga ccgcccgaat taacgatgta atcacagtgc tgactctttt caaatggaag
     7501 gagcagtcag gtttcgggtc cgttttgagg cgggcttcca gcttcgaagc gaaactgaaa
     7561 caggaaacac aaaccatgcc agatacttct cccaatcctc caacaaggaa aatgctcctg
     7621 atcgacgatg atcccaaact gctgttgggt ttgaaggcgg tgatgacgat acagggcttt
     7681 gaggttttct ccaccattga cggcaatgaa gggattcgca tggcgcggga acaactgccc
     7741 gatatcatca tttgcgatgt gatgatgccc aagcccgatg gtttcagcat taagcggttt
     7801 ctggcaggca acccacagac tgccacgatc cccttcattt atttaactgc gcgcacgttc
     7861 acagcggata agatcgcggg gctgcaatta ggcgccgatg attatgtttc gaagcctttc
     7921 aatgccgatg agttggtctc gcgggttgaa gcgattctgc gccgcggcaa tatcggccac
     7981 cagcgcggcc tgcgggaaat ggaagatcgg ctcgaacaaa tgcgcaacag catttcaaca
     8041 aatctggcgc atgaacttcg cacaccgctc acggtcatca tggccaactt gaccatggca
     8101 atgaaggaaa agtttcacgg cagggccgac gatatgaact ggtatctgga aagcatgttg
     8161 agcagcacac aaaaactgtt caagttgacc gaggatatga tcctcttgaa tgacatcgac
     8221 caggggaaac tcagttcaaa acgcattccg atcaatttga acgatggtct tttgaaaccc
     8281 atccgtgatg tgacgacccg ttaccaggta aagaatttgg atgttcaagt ctcagtgcag
     8341 gaagatatgc tgttgtacgc atctgaaaat gatttcagcc gtgcggtctt tcatttggtg
     8401 gataatgcct gcaagttcag ccctgatggt gcgcgcatca tcattcaact gcgcagaaat
     8461 ggatttggag gctgcatgtt gagcgtggaa aattcagggt catccatctc cgtggaactg
     8521 cgcgaaaaag ttttcgaacg atactatcag atccaacaag gcgatgaccg tccatttgaa
     8581 gggctgggga tcgggttgac cattgtccgg gcagtggctg aggtctgcgg tggaagcgcc
     8641 accatcaaag actctgatat gggttgtcgt gtgctgttca ccctgccgcc catgttcgaa
     8701 aaataagcct tgccttgtga attgaaaatc gctcgtggcg gccaaaatgc attccttcac
     8761 cgcccttttt caggaggtca aataatcccc tttttgggga ttatttattt acgccgtctt
     8821 tagttttgct tcaccctaca tttacggaca taaaaagggg gttcaagtat tatcacagca
     8881 aggatacacg acttgtcatg accatttctg ttgcccaggc acagttattc tttttggctt
     8941 ttacccgcat catggccatt ctggttcacg tgcctgtgtt tggcggacaa aacattccct
     9001 cacaagtgcg catcgggctc ggattggtgt tggcgtttgt gctgctcccc tggcagcccc
     9061 tgccgccaga ggcagccgcc atcggtacct tcgcatacag ccttgccatc ggcagggaaa
     9121 ttttgatcgg caccctcatc ggctttgcag cagacctcgc cttcggtgca attcaaatgg
     9181 cgggtgcggc catgggcaca ggcagcggct ttgaatccag ccgcattttc aaccccgcac
     9241 ttggcgaatc aggctcagcc ttcgaccaga tctttgtaat gaccgccacc atgatcttcc
     9301 tggtcatcaa cggtcatcac ctcgttttga tcgccattca aaaaaccttt gaggctgttc
     9361 cgctgaatgg cagcctgccc ttcgacggaa tggaaaccct gctcaaaacg acttccgttt
     9421 tcatcgccgc gggcattcac atggccatgc ccatcatcgc ggccctagtc ctcaccgacc
     9481 tgaccctggg cctgctttca agggtggcgc cacaggtaca ggtttatttt ctgggcctac
     9541 ctgtgaaagt ggtggttgca atgatcgcca tgagcctgac cttttctgtc atctttccgt
     9601 atgtgtcgtc ccttttcaag aacatggctg aaagcatgct tttatttgtg gagtgacctg
     9661 acccatggca gataaaaccg aagccccaac cccccatcgt ttacaggaag cccgtgaaga
     9721 aggccaggtt gtccgcagtc aggaagtgat cactgctgtg gtgatcctct caggtgcttt
     9781 tctgctgcgc ggaccagggt tgcagcttgg gttgaaattc caatctctgc tggtcgaaac
     9841 catcacccaa ctccctacaa ctgaagtcag tgtcgagtcg gtgcgcggca tgttcttcct
     9901 ttatggcgcg gccatcctgc ctaccctggc ctttttgctc ttcgggttga tgctggctgg
     9961 ggtcgccgtc accgcagggc aaacccaatt tttatgggca ggcaaaaaga tcggattcga
    10021 tgcgaagcgg ctgaatccca tcgaaggctt caagcgaatt ttttccacac acggattgat
    10081 cgaactgctt cggtctttac ttaaattagg ctgggtgacg tgggtggcat acagtttcct
    10141 acgctcgagg tttttcgaac ttgtctcact cactcaattt gacttgagaa cctcggtcaa
    10201 cgattttgtg gatatctgcc tctcactcgc catccgtgtc ggcagcatgt atctcgtcct
    10261 tgccgtggct gattatgcct accaacgctg ggaactgtac aagaacctgc gcatgagcaa
    10321 ggatgagatc aaacaggaga tgaagcgctc cgaaggagat ccgctgctga agagccgcat
    10381 ccgcgggatg cagaggcgca tggcccgtgg acgcatgatg gcaaatgtat ccaaggccac
    10441 tgtcgtcgtc accaacccaa cccaccttgc gattgccatc gaatacaacg aaggcatgaa
    10501 ggcacccaag gtgctggcaa agggacccta ccacgtggca gaaaagatcg tgcagatcgc
    10561 gcgcgagaac cacatccccg tggtgcagaa tattccgctt gcccgcgcca tttataaaac
    10621 catcgaaatc ggccaggaga tctcctcaga tctgtatctg gccatggcag aagtactggc
    10681 ttacgtttac aaactgcgag gcaaaacagc cttcgcagaa aaaggaattt gacatgaatt
    10741 caattgcaaa ccacggagac acagaggcac ggagattcct ttttggtttc ctccatgttt
    10801 tcgtggcttc gtggttcaaa tccgtgcctc caaactgcgc ggcaaaacag ccttcgcaga
    10861 ctccaattac taattaccac ttaccaatct aatgaccaca aacaccatca ccccgcccgc
    10921 tccgaatccg atcattgccg cactgcaaaa cctgttgcgt tcaaaagata ttgtcatggc
    10981 gctcagcctg gtcattctgg ttggcatgct gcttctgccg ctgcccgcct tcatcattga
    11041 ccttgccgtg gcattaagca ttgcagtttc catcggcatt gtgttgttga ccatgttcat
    11101 caaacagccg atggaatttt cggtcttccc cacggttttg cttttggtca cattgttccg
    11161 cctcggaatc aacgtctcgg tcagccgcct gattctgctc agcggcgaag cgggacagat
    11221 catcaccacc tttggcaatc tcattgtcgg cggcaattat gtggtcggtg tggtcatctt
    11281 cctcattttg atgatcattc aattcgtggt catcaacagc ggcgcaggcc gtgtggctga
    11341 agtggctgcc cgctttaccc tcgatgccat gccaggcaag caaatggcca ttgatgccga
    11401 cctcaacgca gggctcattg acgaaaacca ggcccgtcaa cgccgcaaga tgatcgaagc
    11461 ggaagcggat ttttacggcg caatggacgg tgccagtaaa tttgtcaaag gcgatgccat
    11521 cgccgccatt atcatcatgc tggtcaacat catcggcgga tttgtcatcg gtgccgttca
    11581 acgcggcatg gacctgacca ctgccctgca aacctatgtt ctgctcacca tcggcgcggg
    11641 gctggccatc caggtacctt cgctgctggt ctctgccgcc gcaggtctca tcgtcacccg
    11701 ctccacctct gaatcatcgc ttggcaccga agtttcggca caggtttcaa acttcaacgt
    11761 gttggcagtc ggtgcgctgg tggtggggtt gatgaccctt gtaccaggcc tgcccaccct
    11821 gccttttgtg ctggtcagtg ttgggctcgg agtggccgcc tatttcgtca accgcgaaca
    11881 ggccaaagcc gttgctgtgg cagaagcagc caccctgctg ccagcccgac ccaccgaacc
    11941 cgaaaccccc gaacaaatgc tggaaatggt cgtcgtggac ccgatcgaac tggaaatcgg
    12001 atacagcctg attcccatca tcgatgaaga caatcaggat aatttactgc gccgcatcac
    12061 cagcatccgc cgacagttga tgagcgaaat gggtttcatc ctccccgttg tccgcatccg
    12121 cgacaacctg cgcctgtcac cgcaatccta tcgcatcaag atccgcggac aggaagtagc
    12181 cgccagcgag atcatgctcg accgtctgat ggcaattcca ggctccgata tcgaagaccc
    12241 gctgcagggc atccccactt ctgaaccagc ctttggcctg cccgccattt ggatcagcag
    12301 cgccgaacag aatcaagccg aagcaagagg ctacaccgtg gtcactccgc tttcggtgat
    12361 ctgcacccat ctcactgaag tggtgcgcgg aaacgccgca gaattactca gccgccaact
    12421 ggtgcaggag atgttaaatc aactccgcca gaaagcgccc gcctcggtgg aaggtgtcac
    12481 tcccgaattg ctcaacctcg gcgaaattca aagcgtcctc cgcaacctgt tacgcgaacg
    12541 tgtgcccatc cgcgacttga gcggaatttt ggaagtgctc gccaacaacg cccccatgac
    12601 ccgcgacccg cacattctcg ctgaagccgt tcgccagagc atggcgcaca ccatctcaag
    12661 tcaatacaaa gatgaggcca acacactgca tgttttcacc cttgcaccgc aattggaaac
    12721 cgccttgcgc gcctcgctcg tcaactcaga aggcagccca ggctttggca ttgatgcggg
    12781 catggcgcag aaggttttac tcaaaacagg cgaacaaatg gaagccctcg cccaactcgg
    12841 tcactttccc atcctcattt gcccgcgcga attgcggctg gccttccgca ggttggtgga
    12901 tcaatccctg cccaacctga tcgtgatggc attttctgag gtcgggcatg gcaccagggt
    12961 gaaagcgcac ggcatggtca aactcgatta gaggtccaca tggtcacaaa aacattcaag
    13021 gcagattcaa ctcttcaaac cctgcaactg gttcaacagg aattcggttc tgatgccatt
    13081 gttgtttcga tgcgcgaagt gcccgaaggc cccatttgga acccgtggaa aaaatcggca
    13141 gtggaagtgg tggccgcagc caaaacgccg caacccgaaa cagtggaaga aagaccagag
    13201 atcgaatggg aactccccgc cgtaaaaaag cccgcgactt tgcccccgcc cctgaaattg
    13261 aacctgaacc cgctgccgaa tccatccgcc ccggtggctg atcctgttca aaaaacagaa
    13321 tcagccgccc cgacgaatga acgctacctt cctccatcgc tgaaaaagat ccagcagcaa
    13381 ttgacccacc aaggcgtgga tgtttccctc gtcgaaggtt tgctaaacct ggcgctggaa
    13441 accctcagcc ctgtcacact tgcagatgcc gaagcctgca aacggtccat cgtgcaattg
    13501 atgggcgcgg aattgcgcgt gcaaaagggt gcgggcaatt tcatctcgaa caatattgtg
    13561 acggtggtcg gccccagtgg aagcggcaag accagtgtgg tggccaaact cgctttgttc
    13621 ttcaggcagg gactgcacaa gtcggtcaca tgggtatgtg ctgacaccgt gcgcacgggc
    13681 gccattgccg aagcaaaagc ctacaccgat gcactcgggc ttgagttgaa actcgtttac
    13741 accccgcagg atttgaacga agccttcaac acctcacaag ccagcgacct gtatctggtg
    13801 gataccccag gctacaaccc ttgcaatgaa aagcaaatga tcgaactcgg cgcactgctc
    13861 tcggaaatgc cctcgcgctg cacctatctg gttgcgcctg ccaccaccaa ggaagcagac
    13921 ctgttccagt tatctgcttc gctgggcgtg ttcaaccttg atggcgtgat catcaccaag
    13981 ttggatgaga cctatcactt cggcggctta tacaattttg cgcgcaagag ctcggtgccg
    14041 ctcagctact ttaccaatgg cagggaaaca gcccgccatt tggaaaccgc cgacccacag
    14101 cggctggtct ctgcgttatt cgcgaaggaa tggaataagt aaaaaggacc aaccccgtgg
    14161 ccgataacga gatctcactg gaacggcagg accctgccga agaagaaaag aaaaaggcag
    14221 actcatccaa acaggcggct gcaaacgctc ctgcccctgt tctggtcgag ttcacgatca
    14281 cggtcagtgt gatttttctg gtgcttgttt tttttacggt gtctggcgtt ttgctggtga
    14341 agggctccac cctgctcgat tttgtatttc gcacaagcgt ttcactggtg gtcatcggcg
    14401 gcctgctggc catcatcgcc cgtcagatca gctcggccac cacgtcacag agaaaagacg
    14461 cacaacctga agaaaccagc ggtcttgacc cttcaagccc gtcagaggtg aagtaaaagc
    14521 atggtcatgc aaatggaagc cacaggaatt ttggaggaat tcatcgcaaa ccgctctcct
    14581 gaattgcggg agaaactggt cctgcaatcc gtgcccctcg tccattattt gctggggcgc
    14641 atgggtgtga cccgtgaacg cgggagcgat tacgaagacc tcgcccatca gggtttgatc
    14701 ggcctgatcg aagcggtgga tcacttcgac ccgaaattca acaccaagtt cagcacctat
    14761 gccagcctga gaatccgcgg caagatcatg gattatctcc gcaccactga ttggatgccg
    14821 cgcgcggccc gcaaacgctc gcgcctgatc cagaaatcca tcacgacgct ctggtcggaa
    14881 aatttccgcg aacccaccga agatgagatc gcctcgtatc tgggcaccaa tgttgaagat
    14941 gtgcagcagg gcctggcaga tacgaaccgt gtgctggttt cactggatat gatgatggaa
    15001 ggcgaccatg aaggtgaagt gcctctccac gaaaaatatg gcgatgaaaa acaggccaac
    15061 ccgctggatc tgctcgaaga aaaagacatg gccgaggata tggcggaagc catcaaaagc
    15121 cttgatgaac gcgaacagtt ggtactctcc ctgtactaca acgaggaatt gaccttcaaa
    15181 gagatcggca aggtgctcga tatcaccgaa tcgcgagtgt gtcagcttca tgcccgcgcc
    15241 cttgtttccc tgaaagcgat gatggaatca tgagcaatat ttccaaagta tccctcgaag
    15301 attcattgta cgtgctgcaa ctggcgcgag agaccgccct ggcgcagaac cgtcaggcac
    15361 aggcaaaacg catggctccg ctggtggagg aaatgcgcgg actggtcgcc aaccctccgc
    15421 aggctgcagc gtcaccctca accggtgtga tgggacaatc tgatttcaag gctttattaa
    15481 atatttcaca ggaaaaaacc agccaaaacc agaccgtcga ttcggtttcg tcggttttgg
    15541 agcgcaaccg cctgatcggt gcgatgtcgg aagccaatat gtcagatgtc gagatcgccc
    15601 gtcagttcag catgagccgt gaagaagttc aactggtatt gagctttcag cagagaaaat
    15661 aaaatggaga gaagaccatg atcaaaggac tatattccgc cgtttccgcc atggtgatga
    15721 atgccaatcg ccagcaggtg atcagtcaca acatcgccaa cctgcaaacc ccgggcttca
    15781 aacaaattct gaccaccgca caggatttca tgccggagca ggcggtgtat accaatggga
    15841 atttacaaac cagcccgatc gaattcgtcg gcacgttggg gcttggcaca caggtcgggc
    15901 aggagtttat tgactttgga gcgggtgcca tgcagaacac gaacaatcct ttggattttg
    15961 cactgcaagg cagcggtttt ttcacggtca aaacccccga tggcaatcgc tacacccgtg
    16021 atggccgctt ccttcgagac gctgaaaata ccctcgtaac agtggacggg tatcaggtcc
    16081 tggatgatgc ggggcaggcc attgaacttc ccgatggaga ggtgtccgtt tcgcccgaag
    16141 gcatcctcag cgtgaatggc accgaagtag ccaccctcgg cattggcgta tttgccgacc
    16201 ccgaagcgga gttggagcac accgaaggca acctgttcac aggtcctgcc gcttcgacag
    16261 gtgaggaagt accgcaggtg gtgcaggggt atctggaaac ctccaatgcc aatccatctc
    16321 aattgatgac gcaattggtg gaggtcgggc gttcttatga agccgcgcaa aaactggttc
    16381 aaaaccagga tgaattgctc ggcaaaacca tcgcttcgct tggacggatc ggataaggag
    16441 tccatcacat gccatcctcc ctttttcaca cgctcaatat ttcacggcag gatatcctca
    16501 gccatatgct cgatctggat gtgaccagca gcaatctggc caacgtcaac acggcaggct
    16561 acaaagccac acgctcgaac tttcaagaga tgctcaatgc ccaactcaag gaagggacac
    16621 aaatgcgcag ttcccaactg ctgaccatgc aaggctccct acgggccacc tccaatccgc
    16681 tcgattgggc cattcagggc gatggatttt tcagcgtcac cctgcccgat gggacgatcg
    16741 gctacacccg cgatggacaa ttctcgctgg atgcagaacg gaccctcgtc aacgccaacg
    16801 gctacccgct cgtttgggat ggcgagatcg ccgaaggcat gaccaacatc agcatttcgt
    16861 ccacgggtgt ggtcactgcc accgatgcgg atggagccac agtagaggtc ggcacagtgc
    16921 aattgacgcg cttccccaac cccagcgggt tgaccgacaa cggcaacaac atcctgctcg
    16981 agtcggaggt ctctggcgcg gcacaaacag gtgaccctgg cgcggagaat ttcggcacct
    17041 tgagcggatt caaggtcgaa aattccaaca ccgaccttgc ccaggaactc accagcctca
    17101 cgatcctgca gcgcaatttg agcatgtcgc tcaaggcctt tcaacagact gacacgatga
    17161 tttcagaggc aattaatctt cgcaaggcat aatgttttta gaaaaagtgc cgataatggt
    17221 attgtgagac cattaacctt gcaggagaac cctacccatg aaaattgaac ccactggaat
    17281 tcaaccgctt tccacgaaag ccaccgaaaa cgccaacccc atcgaaaaac gcgatgaggc
    17341 cggcgaggtg gaatctacgc gcggcggaca ggacaaagtg gaaatgtctg aaaatgcgcg
    17401 cttgctggcg aaagcccggg ccgccctcgg caacaccgaa gaggtggaaa gcgagcggct
    17461 ggcaatgctc aaacaacaag tgcagagcgg cgattacacc gtgcaagtcg gtgaactcgc
    17521 ccgcaagctg ttagcaaaac tatttcccaa gtaagccaac catgaaggac tcctgcgatg
    17581 gtcaatgaag aagaactcac aaaacaacgc tatgaactgg aacaggtact ggtgctgctc
    17641 ttccgcgcac tgcaagacct gctggcactc tctaaaaaag aacgcatttc cctcctgaac
    17701 gaaccgaatc agatcctgca gatcgtggaa gacaaggaag ccctgctcga caagatcagc
    17761 atgctcgaag acaagtgtcg tcaggtcgtt caggcattat cccttgcact tgatgtccaa
    17821 ctggcggaaa cctccattca ggcattattg cccttcctca aacccgaaga cgccgcccgt
    17881 atcaaaaacc tcaccgatgg catttacagc ctcaccagcc aggcacgcga gctaaatcac
    17941 gccagtcagg ccctcgccct caccaagctc gattggctga aggcgaccca atccttcctc
    18001 atagacatct tccaacccga ggcgggctac cgctctccca aagatgcgag caaacgtcag
    18061 gaacctgcag caggtttggg cgtggaaaga cgcgcttgag gcttcaccat gccaaccatc
    18121 tcctccggta tctccatcgc cttgcaagcc gtcctcaccc attcgcaggt gttggaaatc
    18181 accgagcaca acattgccaa tgccagcaca ccgggttacc gccgtcaatc ggcggtgctc
    18241 acggctgaaa caccttcctc catcaacggc gcagaatatt acaccggagc aggacagcgc
    18301 ggcgcgggcg tcaccattga tcggattcaa cgcttcaaca tttcgtattt cgacgggcgc
    18361 tatcgcgccg tttcctccga agctggaaac tgggaaaccc aaagcggcat tctctcacaa
    18421 ctggaatcgg tcctcgccga aacctcagcc gatggattgc tcccaaaact tgaccagttc
    18481 tgggctggct ggcaatccct ttccaacgac cccaccaaca ccagcctgcg gaccgtcctt
    18541 ctggataatg ccgcctcgct ggcaaccgcc ttcaaccgcc gcgccaccca gatcatgcaa
    18601 ttgcgcagtg accagaatct cacggtctca agccaggtgg aagagatcaa cactttggca
    18661 ggcgatatcg cccgcctgaa cgaaaacatt tcgcgggtga tttcggttgg cgaacaacct
    18721 aacgacctgc tggacaagcg cgatgtggcc cttgaccgtc tcgccaaact cacgggggcg
    18781 gtttccaccg agcaaaagaa cggagaggtg cttgtctcgg tcggcgggca tgtacttgtg
    18841 gtggggcacg atgcgttcac gctcgaaacc cgcgccaacc ccagcgacac cagtattctg
    18901 gatgtgtatt ggagcgataa ccaacaatta accccgccca gcggcgaact gaaaggcctt
    18961 ctcgaagtgc gcgaccgtgt attggtggat caattgaacg ggcttaacac tctggcaggc
    19021 gggctgatct cacaagtgaa caccattcac aacagcggct acggcttgaa caatgccaca
    19081 ggattaaatt tcttcagcgg cagcgatgcg ctcagcattt cggtcaaccc agcggtggat
    19141 gcggccagca ttgccacttc cagtgcggcg aatcaggctg gcaacaatga cattgccctg
    19201 cagatcgcag gcttgaaaac ggtcaaaggg atgaaggcag gcacagccac attgaacgag
    19261 ttctacaatt cccagatcac cgaactggcc gtactcacca aacgcgccac tgaccattcc
    19321 taccagcatg ggcttgtcac agatgccctc ggcaaccaac gtgagtcgat ggctggggtt
    19381 tcgttggatg aagaagccgc caacatggcc aaggcccaaa aagcctatca agccgccgcc
    19441 cgggtattga ccgcctacga tgacctgctc gatctggtca tcaatcgcat ggggcgggtt
    19501 gggctgtaag cccagggagc tgatatgaga atcacacacc aaatgatgac cgatggcgcc
    19561 attcaaaaca tggcagataa tctggaaaag gtaagcaaat tgcagaacag aatcgcttca
    19621 ggcaaaaatt ttcaggtcgc ttctgaagac cccacacacg cctcggcgag tctgagtctg
    19681 cgctcccatt tgcaaacgct cgaatcgtac accgacacag ccgaactcac ccaaaactgg
    19741 atgaccgcca ccgacaacgc cttcgaccaa ctggaacagc ttgccgttcg ggccaacaac
    19801 ctcatcctgc gcggattgaa cgactcgctc tcaggcaggg aacgcgccga gtcactggcc
    19861 accgaaatgc aaaccctcat taaccaggcg gtggaattgg gcaacacctc agtcaatggg
    19921 cagtacattt tttcaggtta tcaggtcaac caaaaagcct ttgccctgag cgatgccgcg
    19981 gccaccctgc ccgattatca gggcaaccct ttcaccccca aggtcatcac ctatctcggt
    20041 gaccagggca atattcagcg cagcctcggc ccggaccaat cggtcacctt gaatgtgcgc
    20101 ggcgaccagg ccattctggg cttcctgcaa aatatgatcc tcgcttcgaa cgccttgcag
    20161 caaaacaaca ttcaggatac aggcgacccc gtaacagacc cgctcacttt ggaatccacg
    20221 ctgagcgcgt tgaaatcttc gcttgaaacc ctcgaccaat accgcacttc aaacggtgcg
    20281 cggatgcgtc aggtcgattc ggcggcgaat ttcctcgaaa ccgtaaaatt ggaaacccgc
    20341 agcctgctct ccaaaaacga agacaccaac atggccgaag gcattgccct gcttgccaat
    20401 cagcagacca cctacgaagc cgtgctggag gtcagccagc gcgcgatctc ggcgctcagc
    20461 ctcttcgact atctgcgtta acatcccacc cactggagca tttcttatgc tggtcatatc
    20521 acgcaagatc aatgaaagca tcaccatcgg agacaacatc accgtcaccg ttcttgacat
    20581 cgaaggagac cgcatcaaga tcggcattga tgccccgcgt gagttgatca tcctgcggca
    20641 ggagatcttc caggccgtgc aggatcaggt caaaatacag gaattactgg cagaagaaag
    20701 caaacccgaa tcgctcgacc aactgcgaaa acttctcgtt tcagaaacgg aagaagccct
    20761 cgcagaaccc gcccagcccc caacgcctcc ttcaaaatga aggaggcgtt ttattttcgg
    20821 gcgtaaagaa tttacccgtt ttttggtttc tctttacggc tgctttacag gaaaaaacag
    20881 agggctgtgt taactttgac ggtgagacat tggagcccca tttatggaaa tcatttttga
    20941 tatcaacgaa gaagaactgc ccatttttct agctgaagtt gatgaacatc tacaagccct
    21001 ggatgacagc ctgctccgtc tggaacatgg agaagcagac cctgaattgg tgcagacggt
    21061 tttccgctcg gcacacacca tcaagggcat gtcgggcatg atcgggcatc aacgtatgac
    21121 cgatctcacc cacgccatgg aaaccgtgct cgatggcgtc cgcaaaaatg atattcagat
    21181 cacaagcaag ctgatcaacg tctgtcttga agcggtggac catttgcggc tgctgcgcga
    21241 tgaagtctgt gattcgcagg tctgtgatgt ggatatgaag gaaatcgttt ttgcgctgaa
    21301 gggattgatc ggcgaagaaa ctccccgaca ggaagcccgc ttcacccctg aacctgaaac
    21361 tggtgccgac cttcccgatg gcatcttcca catccaggca gtgatcgacg tgaacagcat
    21421 ggcatctgca gcgcgtgcct ttcaattgat gatggccttg caggatgcag gcgaaatcca
    21481 aacgatgacc ccgtcacaag cggagatcga atcagcggcg agtgtgcgcg agttctcagc
    21541 cacggtggca tcttcgcaat cgttcgaaca ggtcagcgcg gcgttgacga gactttcagg
    21601 tattgaagaa atttcggtca atggcaggct cgtgctttcg ggtggaaaag gcattttgca
    21661 gcaggagcag gcgtcggtta aagtgggagg cgatcgctcc aaggcgggtg gtgtggagcg
    21721 gcgcagtcac aatccatttg gcaggcgcag caccgatatg gccatccgca tgaatgtgga
    21781 acgccttgat aatcttgtca atctggtggg ggaactgatc acagaccgaa atcacttaag
    21841 acagatccac agccgcatga ctcgcatgaa cagcaactac gacatcatta cagaaaccat
    21901 cacgcatttg ggccgcatca ccgatgaact gcaggaagaa gtgatgcaca tccgtatgct
    21961 gccgatcgca agtgtgttcg gcaagttccc gcgcatggtg catgatatgt cgttgaaggt
    22021 tgggaaacag atcgacattg ccattcacgg tgaaaagacc gaaatggacc gctccatgct
    22081 ggaagagatc aacgacccgc tcattcatct cgtccgcaat tctgtggatc acggcatcga
    22141 aacccctgag gagcgcattg cagcgggcaa accagcccgg ggcaagatca ccctcaccgc
    22201 ccgccatgaa cagggacgca ttattttgac cgttgaagat gatggcggcg ggatcaatac
    22261 agacaaatta cgcaagagcg ctgttcaaaa aggcctgatc tcggaagaag aagctgccag
    22321 cctgaacgaa gagcaggcac ttgacctgat gttcatggca gggctttcca ccgcctccaa
    22381 ggtgaccgat atctccgggc gcggcgtggg gctggatatt gtgaagacca acatccagcg
    22441 cattaacggg tccatgcagg ttgagaccaa ccccgggcag ggaacacgtt tccatgtctt
    22501 tttgccgctc acccttgcca tcgtgcccgc tttgctggtg cgcgtgcagc attccacctt
    22561 tgccattccg ctggtgatga tcaccgaaac cttccgcctt gatgaatctg agatcaagca
    22621 tgtctatcga aaacctgtca ccatgctgcg ggagagtgtg ctttcgttga tcgacctctc
    22681 tgaaattttc aatctgccgc gcagcaggga acagaaaaag agcatgtacg ccgtggtggt
    22741 gcagtggggc cgacagcggg tgggtgtgat cgttgattcg ttgttggggg aggaggatat
    22801 tgtagtgaag ccgttgggga gtttcatcgg cgatatccct gggatttcca gcgcagccat
    22861 ccttggagaa ggtcaggttg cgttgatcgt ggatgtgttt ggcctgttca aactggccgg
    22921 aatatagtga ggatgtatgc aggaaacagc acaatttgat gaaaaacttt tggaaatgct
    22981 caacaccatc gcaagtgaag ggatcaagaa tgccgctgat ggtttttcag gaatggtcgg
    23041 gcataagatc gaagtcagcg acccggtgct tgaactggtc cccctgttga cgatacccaa
    23101 ggtcgttggt gaccctgaaa atgatgccgt aggagtgtac ctgcgtttcg aaggggatat
    23161 ggtggggcag atcatgatga tcattccgca ccacaaagcc ctcgaacttg ccgatctgct
    23221 gatgggagtg ccgcagggtt ccacccagca tctgggcacc ctcgaacggt cggcactggg
    23281 tgagttggga aacctgtgcg gctccttctt tttgaattct gttgcaaaga cggtcggtgc
    23341 cagtttccgc cccagccccc ctgccatcat ggtcgacatg gtgggagcga tcctcgatat
    23401 tgtggtggcc acggtcggcg gtgtcaatga gcatgtgctg ctggtacatg ccaacttcat
    23461 ggatggtacg cgcagtgtcg aaaccgattt ctgggtcatc ccggacatga aggccttgca
    23521 aaatttgatg acagcaaaga aaactgacgt atgagcgatc caatcagctt gggattggga
    23581 gaactggccg caagccataa tcccgaagat gtactcattg cctatggtct gggctcctgt
    23641 gtcggcatag cgatgaccga ccccctggcc catgtggcag gtctgctgca tgccgttctg
    23701 ccagaaagtg caaatggcac agaaaacgat gaagcatcta cggctgggaa atatgtggac
    23761 accggcattg caaatctgat cgaagccatg atcaaggcag gcgcaaacaa aagccgactc
    23821 attgtccgca tggtgggcgg tgcgaacatg ttggtcgcgc ccggcatgac aagcaccttc
    23881 gatatcggaa cgcgcaacgt ggaaaaagcc cgtaccaccc tgcaacgctt gaacatgaaa
    23941 atcacagcgg aggaagtcgg cgggcacacc gggcgcacgc tccgtgttta tgtggcagat
    24001 gggcgggtaa cggtgcgcgt catcggacag aaggaacatg aaatataaaa acctcaatgg
    24061 aacaacattg gcagacaacc aataaactaa aggagaaaat cgtatgtcga aaatattgat
    24121 cgtggatgat gcagaatttc tacgggtgcg cttgaccaaa atgctcagca ccgacggcta
    24181 tgaagtcttt caagccgaga acggccttaa agccgtcaac agctacaagg aactccaccc
    24241 cgatatcgtt ttgatggatg tgaccatgcc cgaaatggac ggcctgaccg ctctgaaaga
    24301 gatcgtcggg ttcgacccca aggcgcgggt catcatgctc acggcactcg gccaggaatc
    24361 ggtggtgctt gaagcggtca aatctggcgc caaggatttc gtcgtaaagc cttttgagca
    24421 agaccgtgtg atgaaggcca tcaccaaact actggcttaa atgtatatga ccgacaacgg
    24481 catttctccc aaatcgctgc gggtaatggt ggtggacgat tctgcgttca tgcgtttttc
    24541 cattattcaa cagctcaacg aagaccctga gatacaggtt gtcggttcgg caagcaacgg
    24601 cagggaagcc ctgcgtctga tcccccaatt gcagcccgat gtcatcacgc ttgatgtgga
    24661 aatgccgcac ctcgatggcc tctccacctt gcgcgagatc atgaaggatt tcccctgccc
    24721 ggtcatcatg gtcagcagcc tcacaaaaga gggcgcctcc gaaacgatcc aggcattgat
    24781 gcttggcgca gtggatttta ttgcaaaacc cgataaccgc ctcgatatcc gcattgcgat
    24841 gacggagatc atcaccaaaa taaaacttgc ggcgaaagcc cgcgttaagc agactcttcc
    24901 caacctgttg aagatcgccc cttcaacagc agccagccaa cccgaaaaag cgattcgtcc
    24961 gtactcgagg caggctcctg ttattctgat aggttcatcc acgggtgggc cgggcgcgtt
    25021 gagtgagatc ctgccgatgc tgccatccag tttgccatct cccattgtca ttgtgcagca
    25081 catgcctgtg ggctttaccc gctcgctttc agaacggctc aacaccattt cgcagctcaa
    25141 ggtaaaagaa gccgaacccg gcgactcgct tttggcggga caggttctgg ttgcccccgg
    25201 tggatttcac atgaccttcg atgaagatga acgggtggcg ctcaaccaaa agccgaccgt
    25261 gcatggtgtg cgccccgcag tggatgtgac ccttaattcg atgaaccgcc attttgggaa
    25321 aaacatggtc ggagtgatcc tgaccggcat gggcagtgat ggagccatcg gcatgtccat
    25381 tttacacagc gcaggcggat atgtgatcgc cgaacacgaa agcacctgtg tggtgtgggg
    25441 aatgccgcgc agcgtggtgg aagccggggc cgccgatgcc gttctgccgc gcccgcagat
    25501 cgccaaagcc atcgagtcgg ttgtgcgtga aatgagcaaa caggatgcaa agcatggaac
    25561 ctgaaattta tacacagatc aagacctcga tcaaacaagc gctcaatatt gaccttgaaa
    25621 gctacaagga agagcagatg aaacgccgcc tcgattcatg gctggtgcgc acacgcatca
    25681 acacatggaa ggaatatttc agcctgctca aaaacgatgc cagtgagctc gaaaagtttc
    25741 gcaattattt gaccatcaat gtcactgaat ttttccgcga ccccaaccgc tgggagagtg
    25801 tccgcaccga ggtgctgccg catttgttga aaacatgcaa tcaggccagc ggattaaaac
    25861 tgtggagcgc agggtgctcc accggggcag aggcctacac gctcgcgatc ctgatggaag
    25921 aggccgcacc ccatcagcgc tattccattc tggcaaccga cctcgaccgc ggcgcactgc
    25981 tcaaagcccg ctcccgtgga ccatacagtg tggaggatgt tcgcaatctt tccagcgcac
    26041 aaaaggagcg gcacatcacg ccagcggctc catactttgc caatgaaaaa ctgcaacgga
    26101 atatccgttt tcaagaacag aaccttttgg aagacccatt cgaaggtaat ttcgatctga
    26161 tcgtctgtcg caatgtcgtg atctatttca ccacagccac caaagatctg ctgtacgcaa
    26221 aattctgcaa agccttaagg cccggcgggg tgttgtttgt gggcggcaca gagatcattt
    26281 caggcgcggc caaattcggt tttcaaaatt ttggaatttc tttttacaag aaaatggagt
    26341 gaacatgcct gctcaactca atgaaatcat ggttggggaa atccccgacc tttatggagg
    26401 tggaaagtgg atgtgaaatt tctaaatccg ttcgttcagg ccgccgtgga agtattgaaa
    26461 acggaggttg gagccaatgc ggtgcgcgga gaagtatcgc tgcataaatc atcgttgact
    26521 tctgatgaca tcaccgtctt gatcaacctg atcggggatg tgtatggcgt ggtcatgtat
    26581 ggcatgtcca cggtcacctg tttgaacatg gtctcaaaga tcatgggaca ggagttcact
    26641 gaatttaaca cgctggccca aagcggtgtg gccgaactgg ggaatgtcat cagcgggcag
    26701 gcaaccattc gcttttcaga atcgggctac aaatcgaaca tctcgacccc caccgtgctc
    26761 aacggcaacg gagtggagat ttcaacgctt gattttccac ggatcgtggt gccgttggaa
    26821 acccaatttg gcatgctcac tgtccacctg gcgctgcggg aaaagaaaaa tggagaagcg
    26881 catctggtgg agagttttat ctcttcgatc aaagcgccgg tccctcaaag tgactcgcaa
    26941 gtaccgcccg ccgccacagg atgaattgga gcaggaaatg aatgtcaagt tcttaaaccc
    27001 ttttgtaagc tcggcacatg acatcctccg aatggaactg catgaaaccg tccaacgcgg
    27061 agaattgcgg cttgaaaacg gttcgtatca caccgacgat gccacggtca tcatttcatt
    27121 gatcggcgcc gtggatggga ccgtttttta tggcatgtcg aaggaggcgg ccattcagtt
    27181 cgcttccgct ttgatgggtg aaaaattcga ggcgttgaac gaactggcgc agagtggtat
    27241 tgcagagttg ggaaatgtca ttaccgggca agccagcatg aggttggcag aagcggggtt
    27301 tgagtcgaat atttccacgc cttcgttgct gatcggcaag ggggcatcca tttccacgct
    27361 tgaatatccg cgtttggtgg ttccattaat gaccagcatc ggctcgttga ccattcacct
    27421 gtcactgcgt gaaaacccgc aggcgaactt aaagaccccg caacttgcag ttcctgcggc
    27481 gttcattccc cccaggccct gaaaatttca ggggacgatc cctgcacagg caactccatt
    27541 tttgggggcg ttgactgttt ccatcttcca atcggcctta tccagcagaa tgcggcaggc
    27601 cagttcgcct gtctgtgagg gattcgaggc ggtcaggtaa acctctgttc cgcttttgat
    27661 ccgaatcgtt tttgaccagg gcacggtcac tgtggctggt ttttcaattg attcgctgcc
    27721 cgcctgcagg gtgatgagcg cgtacccgcc agaggcgttc acctcaaaag aaaccaggtg
    27781 actctctgcc gaggtctgaa aaagccccat cgatatcagg aaagccgccg ccgcacccac
    27841 aagtgccaaa acgaacacca cttgaagtgc cgtctttgtt ccatctgaca ggtttgtgaa
    27901 catgtttttt ctcctgttcc ataaataatt atataacagc gttcctgctt tgacttcgaa
    27961 tgcagggtgg gtgcaaggaa aaattaatgc cttatttacg aggaaactgt caatttgcct
    28021 caagtaaggg gccgccctgc cgaaactata ggtgtgacaa aaaaactgaa tacccaaatc
    28081 ctggcaagac ggaggcgcgt atcgaatacc ccacactttt cacctcaatc gacaagtctg
    28141 tacctgaaac aaaaagcaag aaaaacatcg ttcaaaaatc aaattcaaat aggggaagga
    28201 tcccctacct ataattcaaa catggaggtt tgaaatggct actgtagata tcacccgcat
    28261 tgcatcgaac atcggcgctc tcaactccct caattccctg atgaacatca acgccaaact
    28321 ggcgactcat cagacccgcc tttccaccgg cagacgcatc aacagcgctg ctgacgatcc
    28381 cgctggcttg accatcgcca ccaagatgct tgcccgctct gagggtttga aggtgacgct
    28441 cgacaatatc ggcgatgcca agaacatgct ctccgtcgct gaaggcggtt tgagcaagat
    28501 gaccgacatc atcacccaga tgcgcaacaa ggctgaacag gctgcttcgg atacgctcgg
    28561 ttcttcagaa cgcgcgacca tccaaaccca gctttcatcc tatgcccaac agctgcaaga
    28621 catcattgac cagaccaagt ggaacggcgt caaattactc gataactcca ccggtacgaa
    28681 actgttccaa actggtgtag atgaaggcga atccaccacc tggactctgc cgaatgcgtt
    28741 ggaccccgcc acactgagcc tttccaaggt tgtagcaacc gctcaggcca ccaccagcgt
    28801 tttgggtgcc tcattcggtg cgaacgccgc tcagtctggc acgctgaaca acatgtccga
    28861 actgtccact ggcgattact cctttgaagt gctcgacaag gccaccgacg ccacacacgg
    28921 caacttcaac ctcgacacga gcagcaaact cgtcaccggc ctcagcggcg tgaccggctc
    28981 cgcctccccc gcttcagccg ataagatgac ctctggtcac tacaaactga cgattgatgc
    29041 cgccaccagc gccaccagca ccagctacac cctcaccaac ctcgacgaca gcacctgggg
    29101 caccaatggc tccatcgcag tatctggtgt tgacctgtca gccacaggct ccagcggcgc
    29161 gctcaccgac ggcactggca caaatgctgc tgcacaactt ggcgtcacat tagccttcac
    29221 aggcgatgcc gcagacctcg tcaccggcca atcgatgacc ttcgaattca tcgccatgaa
    29281 cgaagtcaaa cttgagctga acgactcctc aggtcaggcc atgcagatca accgcgttgc
    29341 cgccgatggc accacgaact actcagcaag ctccgcttac gtcgccggtg ctggcaccta
    29401 ccagtctggc cgcggcgcta gcgcaacact cgccgcattc ggaacagttt cctccacagt
    29461 cggcaacaag ttggccttca cctacgaaca agcgaacaac ttctcggtga acgtctccaa
    29521 cgctgccaag gctggcgcct acctcaccac caccaacaac gccctcgaca aagttaccag
    29581 cgccctctcc gacctcggct cactgatgtc ccgcctgacc ttcaaggaag aatccatctc
    29641 caccgctcag gtgaacgtgg aagcaggcta caaccgcatc atgaacgcca acatggccga
    29701 agaacaaatg aatgcctcga agtacaccat cctgcaacag acggcgatcg caatgcttgc
    29761 gcaggccaac caggccccgc agaacctgct cagcctcttc cgctaggatc aacggaacct
    29821 ttactgaacg gcacgaaatc catggggagg cgccagtggc gcctccccag caaagtttca
    29881 aaggaaactg aaggtgaacc atggctacaa caatcacatc cattgatgct tccatcagaa
    29941 atctgacacc ctcttttcag accgcgatca aaacaacgat cgaggctgaa agcgcaccgc
    30001 ttaaacgggt ccagaccctg aaagacagca tggaagtgcg ccgcggcgtc tataccgatg
    30061 tcaaatccaa cttcgatgca ttgcaaagcg caattcaagc cctcatcagc acacaagcca
    30121 cctatggatt aagcacggtc gccaaagcca gcgtcacccc tgccatcagc ggcagtaccg
    30181 ttctcaccgt cagcaaaacc agcgaaaccg ccccagtgac cgattacgat ttcacggtca
    30241 ccaaattggc gaaagcccaa agccgcgcca ctgttgcggt agcaaaccca gacctcgcct
    30301 tgaacaagag cggaacattc tggatgggag gaacaggcag cgccgccctg caaacagaaa
    30361 cagcccccgg cgaatacggg gactttgtcg cctccacctc actgacagct gctactgttt
    30421 cgacaatcgc cagcggccag aaagaattag gcagcggcga ctacaccctg caagtccgcg
    30481 attcatccgg tgtacgccag tttcggctgg tcaatgccga tggcaatgcc gtctcgatcc
    30541 gtcagaccga tggctcaacc aactacaccg cagactggca aaccatgacc gacggcgcct
    30601 tcgacaccgg acgcggccaa accctcaccc tcgataaaag tggagacctc gaaagcacca
    30661 ccttccacta cacagcgcgg ggcgtctcca tcaccatcaa cagcagtgac acccagcgca
    30721 acatcctcaa tgccatcaat gccgccaacc aaccagaagg acacgacttc aaagcctcca
    30781 tcgtcgccag tcaattggtc atcaccgcca atcaaacagg cacaaatcac accatgctct
    30841 acaccgatgg cgccgggctg ggattcaatt ccctgctgca agacgctcaa aacgcccaat
    30901 tcaccgtcaa tggcatgagc gtttcccgcg ccagcaacaa caacctcact gatgtggtgg
    30961 aaggcctcac actctctctt gcaggagatg ctgaaggcaa gagcgcgcag ttgagcatca
    31021 atgccaatac cgataaagcc gcagggctga tgaatgccat ggtctcaaag ttcaacacaa
    31081 ccatcaccca cttgaaagac aaaatggcat ccactgccaa gaccgaaaac ggcaagacca
    31141 catatacccg cggcgccctt agcggagaaa cggtcttcag cggttttcgc tctgaaatgc
    31201 tgtatcgcat gagtcgcagc tacaccaata gcggcagttt caaacgtttc gaagaggtcg
    31261 gtctcacctt cgataaggac atgaaactga ccttcgacag cgccaaattt ggtgaagcgc
    31321 tcaagaacaa cgcctccgat gtcaccgccc tgctggatgc aggcatgggt gagatcaaca
    31381 ccacgctctc tcgctacacc ggcaccagcg gaatcctcac caaaacatta accacccttg
    31441 aatctcaagg caaggaatac gaccaacgca tcgcccgata caacacattc ctggaaacac
    31501 gcaaacaaac cctttacaaa caatacatgg aatatcaaac ccagttggta gaccttggca
    31561 acactgccat catgttcggc atcaacctcg gctcaagcgt ggacacctcc ggctaaccag
    31621 ccgaatggtt ttctggtcca accgtcgaac ggcctgtact tctcaaggtc agcgataagg
    31681 atcgtatctc atgaaccaat catccatcaa acccatcagc gggatccaat catcggacgg
    31741 gagcgccctc accaaagcat tgaacaccgc ccagttggaa atgaccgcgc ttcaaccccg
    31801 ccctgccaca actgcgcggc aggaaaaaac gcaggctgaa accaggcagg aatccaccga
    31861 gctgggcagc ctggcaaatg tatccattca tttccgtgtg gatgacaaga ccagcaatgt
    31921 caccgtcttt ctggtggatc gaaaatccaa aaaagttttg cgctccatcc ctgccagcga
    31981 attacaaaaa ctccagatcg gtgacctgtt gaaactcact gtttaaggat cgctcctgca
    32041 gaatttatgc agaattaatg gctcaacgct tcagttcaaa aaaagccagc cgaaactgat
    32101 tgtatggaac aaaagaaatc tttttggaaa aaatcaaaat agaggaatca agccatgtac
    32161 cagtcagcat atcgcaatca atatcggcaa caggatgtca tgagcgccag ccccttacgc
    32221 ctcgtaatca tgacctacga tctggccatc cgctcttgtg aacagcagga ttttggcaaa
    32281 gcggtcaaga ccatcagcgc tctccgcgac gcccttgatc tggattatcc tgaagtggcc
    32341 gctggcttgt tccgccttta tcagtggtgt ctcgattgca tccgcaaagg cgattacacc
    32401 tctgccatca ccaccctgac cgagctgcgt ggagcctggg tagccacaga agagaagatc
    32461 gccgcccgcc aaaccaccac caccgttcaa acctactcca cctcttttgg caacatcctc
    32521 acctgatttt gcccgtttgc atcaaaaaaa cagcccaaaa catacagaag cgcctttccg
    32581 aaaggaagct ggcgctttgt ttttattcac acaaaattta ttttaagttc acactgcttt
    32641 tactgtgcgg atttacctcc tttgataata tcgaaccgac gaaggagaaa cgtatgagct
    32701 tctggacttc cctccgaatt ggtttatccg ggttgacggc acaacgcctg cgccttgacc
    32761 tgatctcaaa caacatcgcc aacgcgcaaa cgacccgcac cgaaaaaggc ggagcatacc
    32821 aaaggcagga tgtcatcttc atgcctgaag agcagaacga ttttctcccg aaactcgtgg
    32881 ctgcccgccg cggacaggaa ggcagtttac agggcggtgt tcgggtggcg gaagtcacca
    32941 ccgatacaga agcaggcccc agggtgtacg acccgaccca tcccgatgca gatgctgatg
    33001 gatatgtcac ctatcccaat gtggatgtgg tggtcgagat gaccaacatg ctttctgcaa
    33061 cacgctccta cgaagccaac ctggcttcgg tagaggcatc caaacgcatg gccttgaagg
    33121 cccttgagat cggcaggtaa tcatcatggc ccttccgccc attaattcca ggcccatccc
    33181 ggccatcccc gagatcaact ccacacagaa accttcggcc agcagcaaca cccagcagat
    33241 cacgcagtct ttcgaagaca tgctgacctc cctcaatcaa tcgcagcaga actccgacaa
    33301 cctgatggag caactggcac gcggcgaaga cgttgacctg cacacggtga tgatcggtct
    33361 ggaagagaac aacgtcaact ttaacgtggc gcttggcatt cgcgacaaac tcgttgaagc
    33421 ctatcgtgaa gtcatgcgca tgcaagttta aattcacacg ggacttttac catgctcaca
    33481 accgttcaac aacaattcat gaccgtatgg aaaaaacaaa gcactgggaa acagatcacc
    33541 attgtggcat tgatccttgc catgttgatc ctcaccccgg tgctggtcaa ttgggcgaac
    33601 acccccagtt attccgtggc ctacaccggg ctcagtgaag cggatgccgc ccagatcgtc
    33661 cagaaacttg atgagaacaa cattgcctat caactcaaaa gcagcggcac catcgaagta
    33721 cccagtgaac aggtgtacac cacccgcctg ctgatggccc gtgaaggcct gcctcaatca
    33781 agcacagtag gttatgaatt gttcagcggc agttccatgc tgggaatgac cgaattctcc
    33841 caaaaggtca attatcaacg cgccatcgaa ggggaattgg aacgcaccat cggaagcatt
    33901 gaagctgtga aggcagtacg ggtccatttg gtgattcctg aaaaaagcct gctcacatcg
    33961 gagcaatcac tcacgaccgc ctccgtcacc attcaagtca acccggggca cctgctggat
    34021 gcctcacaag tgcgggcgat cacccatctc gtcgccagca gtgtggaagg gctcaaaccc
    34081 gaaagcgtgg tcgtagtgga tagtgacggg aatctgctcg cctccggcac ggattctgaa
    34141 actgaactga acctcaccat caaagacgac cagcgcgcca tcgaaatggc ctacgcaact
    34201 gaagtgcaaa aacgggtgca gtccatactg gataagatcc tcggtcccaa ccgctctgtg
    34261 gtacaagcca ccgttgaaat ggattggacc cagcgcgaga tcacatccaa taccttttcg
    34321 ccgaccgagt cagccatccg cagttcacaa accataaatg aaagctccac gagcggcgac
    34381 acaaccggcg gagtaccggg ggcagcctcc aacctgccaa cgccggtagc aactgccgca
    34441 ggcacgggct catccaccta tcaacgcagc gaagagacct tgaactacga agtcagccag
    34501 gtgcaggcgc acgaagtcac cgcacccggt gagatcaaac gcatctcggt ttcggtcatg
    34561 gtggatggca tcacagacca ggctcaaatg gacatcattc gctcggccgt gcaggtggcc
    34621 gccggtatcg attcaacacg cggtgaccag atcgccgttg aatcctttga cttcgaccgt
    34681 accgccctcg atgaattgag cgccgaactt gaaaagcagc agcagcagga actttacatg
    34741 cagatcggca tggccgtagc cgccgccatt gtggtgctgg cactgctctt cttcattctg
    34801 cgcctgatca ataacacccg caatgcttca aaagattcat ggaaagccgt catgagacct
    34861 gtgggagaag tggccgcact caccgataca cactacaagg aagccgcact caaactgcag
    34921 gacgatctct ccacagcatt gtcacgcctg gctgagaagg aaaagacgaa cacacctaga
    34981 gttgaaacca atgaagatgt cgccgtacaa cttcgttcaa ggaatcaatc tgccaaggcc
    35041 attgaagatg agcagaacgc ccgcatcatc tcgaggctta cagaagaaaa ccccgcaacc
    35101 gtggctgaga tcattcagat ctggctgaac gagggcaaga agtcatgacc actggcattg
    35161 agaaggcttc cgcgcttcta ctcggacttg gagtggatcg ctcatcccgg gtgcttcagt
    35221 acctgaatga gggtgagatc gaacaagtgc tgatggtgct cggcaaaccg ggcacggtcg
    35281 caccggaagt gcgccatgaa gccctgcggg aagcctgtga actcagcctt tcagacacag
    35341 cagaaatgcg cggcggaatt gaatacaccc gccagcttct ggctcgcgca gtgggtccgc
    35401 gccgcggcac ggaaattctg gaacgcatct cagttcacca gcagctttcc tccttcgaaa
    35461 tgctaaagaa tgccaacacc accgaagtgg ccaccctgct tcaggaagaa atgccacaga
    35521 ccattgcact cgtcctttcg tatctcgaag cgaaagtcgc tgccgatatt ttgacgagca
    35581 tgccaaagga aattcagatc gaggtgaccc tgcgtctcgc cgcgatggat agagtctcac
    35641 cgcaggtggt tcaacaggtt gaaaaaagcc tgaagagcaa actttccagc gtgatcaacg
    35701 aagccgattt tcgtgccaca ggcggcgtga cctttttggt gaaggtgctc aaccaggtcg
    35761 atcgcggcgc acaaaaatcc atcttcgatg cgttggaaac gaccaacgcc aaactggtgg
    35821 aagagatccg cgccaacatg ttcacctttg acgatgtcat caaactcgat gaccgcgcca
    35881 tgcagcgtgt gttgcgcgac atcaacaaat cagaccttgc tttggcgctc aaaggcgcac
    35941 ccgaccgcct cagagaaaaa gtattcgcca atctttcaga acgtgcccgc gaaaacctca
    36001 aggaagaaat tgacatcctc ggaccgcagc tcgccaaaaa cgtgtacacc gcacaacgca
    36061 agatcgtgga tgtctgccgc tcgctggaag aatctggcga gatcgtcatc gctggcggag
    36121 gaggggaaaa tgaagtcatt gcataaggcc aaaccaaatt ccgcattgga agcctggtca
    36181 ccgcctgaat tggatgccga tgcatcggtc aacgcacagg ccaaccgcaa ggaacaagtg
    36241 cttgccatct ttcaaaagga agaagagggc aaagcctcat cgcaggaaaa ttccattctt
    36301 cgaggaggaa ccgcgggaaa aaatctgaca acatggcagc ccggtcttct tgacgatcac
    36361 aacgcctcca cccgcgccga caactggtcc ttcatcgaag tgaccgatac gccctttgac
    36421 cgctcttgga aagtgcaatc gcccggcgtt ttcgtaaaag aaacgtctca acgagacatg
    36481 ttcttttcag aaaaagaaat tgcaaccgcc ctcgaacgcg cacggcttca agcggaagaa
    36541 atcatccttt cagcccaggc cgaagccgat gacctgcttt tacaggctca atctgaaatt
    36601 gaccatcaaa agaaggaagg ctatctgcag ggaaaaaatg aagcccgcgc tgaaatcgaa
    36661 accgctgccc aggccattca aaccatggtg acagagatcg aaacatggaa agccgaattc
    36721 ctttcgcaag gcgaacagat cctgctcgaa atgttgaaag acatggcgag gaaaatgttc
    36781 ggcgaaggcg ccaaattgga tgcccctgcc ctgcatgcca acctcaaccg cattattggc
    36841 aacgcccacg gcctcggaat gctcaagatc tttctcaacc ccatcgatgc caggcagttg
    36901 gaccccttct ggagcgaaca gcaaatgctg attttgggcg agcaagtgaa gatcgtccct
    36961 tccaacaaca ttctgccggg cggctgcctc atcaaaggca acatgggcac cgtggatgcg
    37021 cgtgtggaaa cacaactcga ttccattcta aacactttcg acgacccgca ggcaaccgct
    37081 gagtaagcca tgcaaaacac caacctcaac ctcgcacgtt ttcgcaacac cctgtcccag
    37141 gtcaacccgc tggcgatcac tggccgtgtc gtgcaggtcg tcggattgac ggtggaaatc
    37201 atgggcttga attgtcagat cggcgaaatc tgcgagatcc aaaccagcaa caaaacctca
    37261 ttgatggccg aagccatcgg cttccgcaac aaccgcatgc tgctgatgcc gctgggaagc
    37321 atggaaggca ttcaacccga tagcaccgtg cgttcggtct cgcaggtctt caaagcaccg
    37381 gttggactct cattggttgg ccgagtattg aacggcctcg gagaaccgat tgacggaaaa
    37441 ggtccattga agataaacaa ctgggcctcc attaatcagt cacctcccca cccgcttcaa
    37501 cgcgctgcca tcgaagaacc actcgtcacc ggcgtccgtg tcattgacgg aatgttaacc
    37561 tgtggaaagg ggcagcgcat gggcatcttt gcaggcagtg gtgtcggaaa aagcaccctg
    37621 ctgggctcca tcgcgcggaa ttcggaaagt gatatctccg tcattgcctt aatcggcgaa
    37681 cgcggacgtg aagtgcgcga atttttggaa cgcgacctcg gacccgaagg cctggcacgt
    37741 tcggtcatca tcgtctccac atccgaccaa cccgcgctca tgcgtttgaa agccgcatcg
    37801 gtagccatga ccatcgccga atacttccgc gatgaaggca tggacgtcat gttcatgatg
    37861 gactcggtca cccgctttgc catggcccag cgtgagatcg gcctttccat cggcgaaccg
    37921 cccgccagca aaggttacac gccatctgtg tttgccctgc tgccccgcct gctggaacgc
    37981 gcaggcaaag gtgaaacagg ctccatcacc ggcttcttca ccgttctggt ggaaggggat
    38041 gatttcaacg aacccatctg cgatgctgtc cgcggcattc tcgatggaca catcatcctc
    38101 agccgaacgc tggcagcccg caaccactac cccgccatcg acgtgctgaa cagtgtcagc
    38161 cgtgtcatgc cagccatcac caccaaggaa catttgcagc tcgctgcctt tgcccgcaaa
    38221 aacctcgccg tcttcgaaaa attccgcgat ctggtcaaca tcggcgccta cgtcaaaggc
    38281 tccgaccctg aagccgatac agccctgctc gtcatgcctg atatcaaccg cttcctccaa
    38341 cagaatcgct cagaggtgag tccgtttcag gacaccctgc aaactttgaa cgagatcagt
    38401 cagaccgccc ccgcaagtta aattgaggaa aaaagccaat ggcacccaaa ttctcactgc
    38461 aaaacgtttt agacctgcgc cacggaaaag tggaactgtt agagatcgaa ctgggcaaac
    38521 ttacgaccat ccaccgcgaa acccaaaccc tgcacgcctc actgcaacaa tatcaggcca
    38581 gcctgctcaa ccaactgaac gatgcccaaa caggcgaatt ggacatggtc aaactcaacc
    38641 tgctgaggct gaacatcctg caactcaaca ggcatatcga actcgtcgtc cttgaattga
    38701 aaaaacagac catggagatc gatgccaaac gcgccgaact ggtcaaggca aaacaatctg
    38761 aagagacgct cgagatcctc aaaagaaaac gcaacgaagt gtacatggca gaacaggttc
    38821 aactcgaagc acaagcccag gacgatatct acatcgcgcg ggcattccga aatcaaagag
    38881 gagcttaacc catcatggat aactcattcc tgacaggccg catccaatcc gtcatgcaga
    38941 atctcatgct caccatgatc aacaaactgc tcacacggct tgaatccaaa tcacaggcca
    39001 atggcacaac aggcacaaaa tcgacggcac agaccacatc cacttcactg gcatcaacca
    39061 gttcagcggg gcaggctgcc agcggaaact ttgccagcct catcaatcaa gcctctcaaa
    39121 aatatgatgt ggaccccaac ctgatccagg cggtcatcaa agcagaatca aacttcaacc
    39181 ccaacgccgt ttcatctgtc ggcgcacagg gattgatgca gctcatgcca ggcaccgccc
    39241 gcggactcgg agtcaccaac tcgctcgacc cagcccaaaa catcgatggc ggcacccgct
    39301 tcctcagtca attgttggat cgctacaacg gaaatgtccg cctcgccgtc gccgcctaca
    39361 atgcaggccc aggcgcagtc gacaaataca acggcatccc gccctatcag gaaacccaaa
    39421 cctacgtcac acgcgtactg ggctacttca actccagcaa cgaatggcag gcgtaagatg
    39481 agcgactcac ttttttcaga tatctccaca cgcacagcca aagctgcact cgacggactc
    39541 agcctgcgtc aacaagccat cagccgcaac atcgccaaca tcgacacccc cggatatcag
    39601 gcacaaaccg tcgattttca agaaacactc acccgccttg tcgaaaacga tgacctgctg
    39661 ccaatgtcca ccacgcacga agcccacctc accaccgccg caagcaacct caccctatcc
    39721 acgtcattgc ggccaggcgg cacccaacgc gccgaccaaa acaatgtgga tatcgatgta
    39781 gaaatggcag acatgtctga agctggaatt caatatcaag ccatcagtca ggccatctcg
    39841 aaaaaactgc tcctcctcaa aacactggcg aaataatgat gcctctcggt cgtcaaccca
    39901 ccgtaacacc cctttcatca gatggattgc ccgcgcaacc ccaattacgg gattctggcg
    39961 cagcacaagc tccatcgttc aaagaggtgt tgaaggttga agaaaaaaaa ctgcaacaaa
    40021 ccaccaacac ttcaaacgcc gccgcattgc ttgccacact gcaactgccc gccatccctg
    40081 caattgagat cacacaactt gttggaacga ataaacccac cgatccctcc gcgctgacca
    40141 ttgattctat tcaaacagaa acagcctccc cgaaacagga ttcgccatta tccaatccgc
    40201 aaaacggaac aaacacaaac caaagcaaac ccgcctacac cccacccatc tcgagcatcc
    40261 caacaacaac caaagctgag caaactcctg acgcgccaac ggaaacccca gccgcaaaaa
    40321 taacgacgaa accaaccaac acagcaaaag cggacacagt tgatattgag gctgtcaaag
    40381 tcaaatccca gccaacacct gtggtgacaa aggaatcgcc caaagc
//
LOCUS       DAOQLC010000021        39873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_282_length_39873_cov_23.277437, whole genome shotgun sequence.
ACCESSION   DAOQLC010000021 DAOQLC010000000
VERSION     DAOQLC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39873
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_282_length_39873_cov_23.277437"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /locus_tag="PL000_05655"
     CDS             complement(<1..1598)
                     /locus_tag="PL000_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88413.1"
                     /translation="MVKKKSDITKNKRTKNTDDQALTHIRQLAWTGQHAAAIDSATEA
                     LENVGAGSPRPGKGRGNRAPTMMSLLDLRAESYIAIGKLDLAMKDAKAMMKMGSKVAG
                     LKVQALNRLALVQMRTGDLKGAVKTAKSTVGARFPRPGRGDPAPTALRAESLYRLAEA
                     QWRARHLDAALETAQKAIDAYQELGDTSGTGRAYWVLSGVFFRSDRAEESRRASQTAL
                     ELCKQAGDSYGIGNALLQLANTDVDIAEQIQHYQQAKSAFETVGYVDRQNAVIANLAN
                     TYSYLGLYAHVRRLRRESIAQGRAMGAKIALIVGLGNLIGTETIFGEVEAARLHLREL
                     ETLVPDLGDPSLEAGLFNQRADLAFATGDILTAIRHQKSALKLIKESQISGEHTTLTA
                     LATYYLAASNPAAALKATSKATALHRAQNFARPEGTGSQYIWWWHAQALIANGKTKEA
                     REALDRTYDFLLEGITNIRDAGLRRNALNKVEDNRKLLQYWVKDGAQRKLPKERLFAH
                     LNIESNLREPFQRLADTGLRLNAL"
     gene            complement(1606..1962)
                     /locus_tag="PL000_05660"
     CDS             complement(1606..1962)
                     /locus_tag="PL000_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88414.1"
                     /translation="MKTILVVEDTELNIDLITQLLEDDYNLLVARDGIEGVSMATEHT
                     PDLILMDISLPILDGYEATRTIRKTLPNLPIIGLSAHAMDGHEQKAKDAGCTDYLTKP
                     VNDDLLFKMLKKYLGE"
     gene            complement(1982..5311)
                     /locus_tag="PL000_05665"
     CDS             complement(1982..5311)
                     /locus_tag="PL000_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88415.1"
                     /translation="MAKKASPVRKAVKKGAKMQATESGQRLKSHPPSAPVTDPSSPIT
                     LAHNLAWMGQHTQAIKLTSLELSKDEHSLATRIGLLNQRVESQLAQGRFDLAAKDADT
                     MMELTEADQGELKVQTLNCKTLVQIHSGDVQDAIKTATLALDIASTNAIRNKRLLAMS
                     HFRMSEAYKNVSQFEAAVTEAQKAMEIFLAEGDLSGAGRASYIMAPSYNVLGFAQKSR
                     HAAQTALELCQQVNDQLGIGNALNGLSSTDPDLAERIHHYQQAIQAFEAVGHLERQST
                     STGNLGFVYAALGLYTHARRLTRKAFEIDKAIGAKIGMAYWSENLFDTELRMGLIESA
                     REHLNEMGRLAAEFGDPRLSASFNTNQGLMLLAEGKTQQAVQCFQDGVQTAREAEIGN
                     ENVHLSYLAIAHLKNNDPVAALAATTAATELHRSQDYKLPDSYSSHEIWWTHARALFA
                     NGKEVEARKALARAHEFLLEAIANVRDEGLRRNCLNKVKENRELLKFWISDNLNASGR
                     RKLSKKKIFAHQYLESNTREPFKRLTDTALRLNALHTTSEMHTFIVEEATELSGGERV
                     LFILEKNGVCEAAESILPRGENVQEVLNLIDPYLTQARLTRTVQLILPANDSDSTLSR
                     IIAPLIAQNQIIGYIYADMDSLYGTFTEVDRDMLGMLANQAAVALDNVNWAQGLELKV
                     QERTAELHARVDELAILNSVQAGLASQLDVNAIYELIGEQLRSLFDSQAISIISFDLK
                     KGTRHYHYMLEQGQRHDIPDAAIPPLSQFLIQNKRPILINENFKERLASIGITSQTVP
                     GTKPTKSLLRVPIMDGDEVRGVIGLDNIDRENAFNNADVRLLTTLASSMSVALEKARL
                     YQQAQEARAAADAASAAKSAFLANMSHELRTPLNAIIGFTRIVRKKSEGVLPEKQIEN
                     LDKVLTSGEHLLGLINAVLDIAKIEAGRMEVHAANFNINALIEQCYNIALPSMKPAVK
                     FDRRASEQLTLIHSDQEKIKQIMLHLLSNAAKFTHEGQVILAVEKVDASAEVPVPCFR
                     ISVSDTGIGISEEALGFVFGEFQQADNSTTRQYGGTGLGLTISRNLANLLGGELTATS
                     ELGKGSVFTLTLPIQFQPKKN"
     gene            5748..6689
                     /locus_tag="PL000_05670"
     CDS             5748..6689
                     /locus_tag="PL000_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88416.1"
                     /translation="MTTIFFATDIHGSDICWSKFLNAGKFYGADKLVLGGDMTGKAVV
                     PFVHQGGKNYRITLLEQVFETTNDEELADMTKRVRSRGYYPYLTNPDEIAELEKDPDR
                     VSKIFLQQVLKVVQQWMDLADKKLDGTDMMVYCSPGNDDMDEVDEIIRSSKRVMLVEG
                     LVSQLDENHEMIASGWSNITPWKTHREENEDQLAVRYEAMYSRLKDPRNSIFNIHVPP
                     HKSGLDEAPELDENLRPVLAGNSMIPVGSTALRKAIEKVQPLLSLHGHIHEGRGATRI
                     GKTLCINPGSMYEQGTLLGAIVKLGKNKIENYVLTTG"
     gene            6742..8388
                     /locus_tag="PL000_05675"
     CDS             6742..8388
                     /locus_tag="PL000_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC88417.1"
                     /translation="MSNLFVRKATGMVRSWSVFDAFIYAFFSINFVTLGFYAFSQMYY
                     FGGGMINALIISAIFLVFEIIVYASLIAVMPRSGGDYVWMTRIFGGSIGFILAITGWW
                     FTLWLWTPVYGDMFRQVVLVPIMGVLGMRDTAVWFAGTGTPLFVSSLITLAFVSLVII
                     LGMKTYARIQKFSFYAGVLGLLIVIVLLFTGSPDKFKAGFEKNAAAIFGSQPGVYDAT
                     VQTGTDAGAATPLTGGSFSLIMVTLPWLVFFNLWPNWGATLYGEVRGATDYKRNFSGM
                     AWALGTVTVLGIVFFLGVAKTIGWDFYMQANGSWWNYYWGYVTDVPPLPVWPYPAMLA
                     TFLTESRALQLIVMVLMSFWWFGWAGTLFLSSTRVVFAAAFDRLLPEKVAELNERGTP
                     VNAMLLMVIPAVIVSYMYAYNVGGFISVTLAATQAIAIMYFGTAIAAIVLPYKKKDLF
                     EASPIAQMKVAGIPLITIAGVIFASFLGFLLVEWFFDPWLNTGGEGAGLYGISFKNTN
                     SVIFLLVTYGLAAAIYYGFKSYRKKSGIDMDKVQTEIPVE"
     gene            8464..9363
                     /locus_tag="PL000_05680"
     CDS             8464..9363
                     /locus_tag="PL000_05680"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNC88418.1"
                     /translation="MPVIDEVVSKMEAWKGRDVSIEHLSGGLTNTNYKVHVDGRPFFI
                     RVPGEGTELLAIDRNNEYHNSRAAANAGVAPQILHHFPEYSVMVLEFLNGKTMSKDSL
                     NQKGMPARMAQSIKKLHAGERFLTDFNMFRLTEYYLRLCKERDIKIPDGYLDRLETVA
                     LIEKAMSVNQLATVPCNNDLLAENYIDDGNQLWLIDYEYSGNNDPTFELGNTCQEMQF
                     NDEQIREVCAAYFGDVSPAMIARMKLNMIMSDVGWGLWAAIQEKISTIEFDFWGWALE
                     RWGRAQEKMDSPDFDIWLKDVRQ"
     gene            9453..10193
                     /locus_tag="PL000_05685"
     CDS             9453..10193
                     /locus_tag="PL000_05685"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC88419.1"
                     /translation="MDIIQEMHRLIDAANGKNIQLRAIGGLAVQSHNKKNHPLFLREF
                     ADLDFVVAKKQRREFEAFMPEVGYAPHRQFNVLNGDQRQIYYHGETDMKIDIFVGDFM
                     MCHKIPLENRLKADPVTIPLAELLLSKAQIVHLNRKDALDIASLLLNIEVGTGNDNII
                     NLAVIAQLCGSDWGLYKTTSINLERVKEVVSADDVALTSDEKSLIVSRVDKVLDAFAA
                     MPKTLAWQMRDKVGTRVRWYEEVEEVAR"
     gene            10190..10327
                     /locus_tag="PL000_05690"
     CDS             10190..10327
                     /locus_tag="PL000_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88420.1"
                     /translation="MSKHLRLFFAVLILTLSISLLVWGYMPNPHETRIQWIAPTEMQL
                     P"
     gene            10324..11070
                     /locus_tag="PL000_05695"
     CDS             10324..11070
                     /locus_tag="PL000_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88421.1"
                     /translation="MKEVLKPLYRLVVFPVFMVVFISLACVGNGNAPSSASEPTAISA
                     LATQTTNEVFPEPAPAIPETRRLTLEFPPKMRAGVEGDIVRLTLEVDDLGNITPTAQF
                     EGNVVTGEVIEIPDLYETHNVTAEARLDMAGMQIQPSGAIYEPLKRGQSATFFWSIRP
                     QDTGVYRGTVWLHLNFTDISTGEQSRIAVSAHTVEIEAVDFFGLSVNVARTTGVVGSV
                     VGGILGFPFLEDIIKFLLKFRRKKQSPLAN"
     gene            11176..11529
                     /gene="ndhC"
                     /locus_tag="PL000_05700"
     CDS             11176..11529
                     /gene="ndhC"
                     /locus_tag="PL000_05700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC88422.1"
                     /translation="MNEWLYVGLFFIVGLIIPVGAIGVGWLLSPKKPNPIKQSTYECG
                     IEPVGEGWVQFKAQYYIFALVFLVFDVEAVFLFPWAVKLNQLGLFAVVEGIIFILILV
                     AGLVYTWRKGMLEWA"
     gene            11652..12845
                     /locus_tag="PL000_05705"
     CDS             11652..12845
                     /locus_tag="PL000_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNC88423.1"
                     /translation="MNFWNDPLKIAADWLTGIFVGWGMNDTAAHVLVAFLGVLILISI
                     LMVVDIFLVWIERKVVARFQDRIGPNRLGPFGLIQPFADIIKLMIKEDTTPDGADKVV
                     YNLAPMLSMMSVLILWAVVPLAPVMLGVDLNAGALFIIAAGSIGTLSIIMAGWSSNNK
                     FSVIGAFRQVAVMVSFEIPMLATLLIPTIFAGSMGMNAIIQGQDIWFVLLSPLAALIF
                     LIAAIAELGRAPFDLAEGESELVSGYNIEYSGMKFGMFYAGELLHAFTFGGFWAIFFF
                     GGYRFFGLEKVSPFLAIAIILLKALVGYWIIMWVKYTVMRIRIDQMLAFNWKFLTPVA
                     FVLVMVTALMNALLREASSGLYITGMILSNIIVGWVSLEIVRSMSRKERERVEGVKQV
                     VEAHH"
     gene            12922..13431
                     /locus_tag="PL000_05710"
     CDS             12922..13431
                     /locus_tag="PL000_05710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC88424.1"
                     /translation="MTAEQIIFLIVAGVTLASALLVVTTRNLVHAALWLVATLFGVAI
                     IYAILNAGFIAVVQVVVYIGAIAILFIFAVMLTRRELRDNTPQLNRNWWSGALVAVLT
                     FAGLASLLSRWSGFSKTAAALPSGFDAVAELGNQLVSPDAFVLPFEVASVLLLAAMVG
                     AVYVAFTRK"
     gene            13444..13752
                     /gene="nuoK"
                     /locus_tag="PL000_05715"
     CDS             13444..13752
                     /gene="nuoK"
                     /locus_tag="PL000_05715"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC88425.1"
                     /translation="MIPLSWYLIFAAALFSVGLFGALSRKNAVAILLGIELMLNAINV
                     NLVAFWRYGDVAVMAGQVFAVIVFAVAAAEVSVGLALVISVYRRRNTVIADELDMLKW
                     "
     gene            13762..15915
                     /gene="nuoL"
                     /locus_tag="PL000_05720"
     CDS             13762..15915
                     /gene="nuoL"
                     /locus_tag="PL000_05720"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC88426.1"
                     /translation="MTHEFVTNLVWLIPLPPVIAFALIVLFTNKSKALSHTVAISAAF
                     LSWLGAMIVFWNAVKVEHFAEEPFAASVNWLPTGETWLKVGVLIDPLSATILFFVAWT
                     VLMIFLYSVGYHNFGQPAGDHDHKGLPPHGATVTDDLGHKHTVLSIEPMYSRFFAFIS
                     LFAFGMLTLVVSDNILTLFVGWEIMGLCSYLLIGFWYGKESARNAAIKAFMTTRIGDV
                     FMLLGISFLYSVTGTLSFREIFTEEILHTLASVPSGVFGLSAAGLIGLLLFIGTVGKS
                     AQFPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYAVIRMFPIISLDEKTMAVVAFIGA
                     FTALFAATIAVAQNDIKRVLAYSTISQLGFMIAALGIHAYVAAVFHLVTHAFFKALLF
                     LGSGSVIHGMEHGVMHTGNHKVDPQDMFNMGGLRKKMPITFWTFLIGGFALSGFPIVT
                     AGFWSKDEILADAFGHGHTVIFGTLAFAAFLTAFYTMRQITLTFLGEPRTKEAEHAHE
                     TPWTMTTPLVVLSVFAVTYGWVGIPEHFPLLGGLVPNWFHEFVGSTLHEHPEALEFSI
                     VPLLTSLGVALGGLGLGWLVYRNVNSPAEDRLQIGLLKNKYYFDEIYDVVFVKPAYWL
                     AENVIYKFMDQGVIDGILHFFGPATGGVGSTIRNYFDIPVINHLIGDGSADATYWIGK
                     NLRPIQTGRIQQYLILSLILLFVVGGLVYYILTLA"
     gene            15925..17448
                     /locus_tag="PL000_05725"
     CDS             15925..17448
                     /locus_tag="PL000_05725"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC88427.1"
                     /translation="MDFLNSHLLSLILFLPTVAAVIMLFLPSEEAKLQRWFAFTASLI
                     PFGLTLYLWNAFDPNRTGFQFQEQYTWYEAIGSNFHLGVDGISLSMVLLTTLLTPLAI
                     LASFSVSDKVKAYFMLFLFLETGMLGVFMALDLLIFFVFWEIGLVPMYFLINQWGSAN
                     RNYASLKFMIYTMAGSLGLLLAVQMLGVLFGTYDLVTLYERWNSLDAGSVLLGMPVHT
                     VKTIAFWAFVVAFAIKVPVWPFHTWLPDAHTEAPTAGSMILAGVLLKLGAYGFIRLVL
                     PFYPLEAKIFAGALAFLAVAAIVFGAFSSYAQTDFKRLVAYSSVNHMGFVMLGIAAAG
                     LASGTQDAHIAMSGAVLQMFNHGLSAAGMFFLVGVIYERTHTRNLNDFGGLFPLVPVY
                     GGILIFTSMASLGLPGLNGFVSEFMVVRGAYPVLTTYTAISMLGLLFTGAYILKGIKS
                     VLHGPLNEHWAHGEHKLTEINTREILVMVPLMVLILVIGVWPMWLLDVINKAVTALF"
     gene            17459..18976
                     /locus_tag="PL000_05730"
     CDS             17459..18976
                     /locus_tag="PL000_05730"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC88428.1"
                     /translation="MTNFPILSVITFLPILAAAIILMMPANRKNEVRAVALAAATLDL
                     LLSGWLYLQYLLGDMTGYQFIEQYNWLPALGISLKFGVDGMSTPLVLLTGIVMFTGVL
                     ISWGDEDPHVKAGIQDRPREFFAFLFLLAGGVFGVFVSLDLFMLFFFYEIAVFPMYLL
                     IAIWGWVKTREYAAMKLTLYLFIGSVVALVGALAMYWTQYQNTGVLSFDMLLMENAKF
                     SPTFQYVWFLPVFLGFAVLGGIWPFHNWSPDGHVAAPTAVSMFHAGVLMKLGAFAALR
                     VGVMLLPEGAKDWAWLLMLFAGVAVVYGAFIAFVQTDMKYMIGFSSVSHMGLVVLGVS
                     TLNHDGLVGAGVQMFSHGVMTALFFAVTGMIYDRAHTRMIPELGGIAKVMPFATVGFI
                     IGGLVSMGMPGFSGFVAEFPIFMGVWGRQWLVAVIASISIVITAAYIMINIRKVFFSE
                     MPENLHGHIGDVTMLDKIAITTLCLFMIVIGMFPAVMVPMVEKGVENVLRLLGGA"
     gene            18976..20424
                     /locus_tag="PL000_05735"
     CDS             18976..20424
                     /locus_tag="PL000_05735"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC88429.1"
                     /translation="MFTSTTFASILPETLILILGIILLIVEPFWKAENRRNVGWLTAV
                     GLFIAMLVSLLFGQPGEPTSVLGGMIRFDWLGFFFKMLFMFAGAATALLLMDHEKAGL
                     RGEAYLMLLASIIGMNLMAVSSDLVMMYLAIETASIPLYILSGFLLGDERSTEAGFKY
                     LLFGTLASTVMLYGFSLIFGFSGTTNIYKLSEMLAVGELPLFVNFGLIGLVVVGLGFK
                     IAAVPLHFWAPDVYQGAPTPVAGFLSTASKAAGFAVIIRLFFVAFPEIANYWTVILAA
                     LAAVTMTAGNLLALSQTNIKRLLAYSSVGHAGYALIGVVAFSQLGAASVVFYLAAYIA
                     TNLLAFGIVMAFSRVAGLEDITDYAGLSRRNPLMGLMMLAAFLSLAGMPPFGGFVAKV
                     FVFAAGVQANYTWLVVVGILNSIIGVYYYLNVLKYVYLYRMPNEDEENHPIPLTRPYT
                     IALVVLALGVILVGTVFAPWFSWSDAAALNLF"
     gene            20668..20946
                     /locus_tag="PL000_05740"
     CDS             20668..20946
                     /locus_tag="PL000_05740"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNC88430.1"
                     /translation="MTQSKQTNRQNMVYTLLVVMVGQVGCLTIAVILASVFGGLWLDN
                     TFGTKPVFTLVLLFAGIPLSVFLMIFAARKTLARLKEKYETEEQKTTA"
     gene            20956..21960
                     /gene="atpB"
                     /locus_tag="PL000_05745"
     CDS             20956..21960
                     /gene="atpB"
                     /locus_tag="PL000_05745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC88431.1"
                     /translation="METQKRTPWRRWIVLVLMIVGFILGGIFVPVQPEITVAAEKLIE
                     EPLIENFLGQPFYLVNTLPTLFVTVFIILLPLAWSAGRSLKKSQETDLVPRGVGNTME
                     AILEMLYNMTESSAGVKWAKTIFPWFATIMIYVLVANLLKLLPGFESIGVIHHAHGEG
                     HLTQELGNFLGFRWELLSPDKVEEGGYILAPFFRGISVDLNFTLSLALISFFAIQIIG
                     VRSQGIGYFSKFFNTKRMFKVPFFGAMDFLVGLLEIISELAKILSFTFRLFGNMFAGI
                     VLVAVVASLLGKISILPAMIMMFELFVGLIQAFVFGMLTMVFMAMATQGHGDEEHAEH
                     "
     gene            22014..22253
                     /gene="atpE"
                     /locus_tag="PL000_05750"
     CDS             22014..22253
                     /gene="atpE"
                     /locus_tag="PL000_05750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNC88432.1"
                     /translation="MEGNILEAMRFVGAGLAMIGAAGAGVGIGLSVQGALTGMARNPD
                     TYGNLLTNMILGVAFSESIAIYCLVIAFLMLFKVV"
     gene            22272..22991
                     /gene="atpF"
                     /locus_tag="PL000_05755"
     CDS             22272..22991
                     /gene="atpF"
                     /locus_tag="PL000_05755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNC88433.1"
                     /translation="MEALGINLGLLIVQLIAFAIVFLTLNAWVYQPMLDMMESRKQKI
                     SQGLEDARVAAEARANAEKEAAKVIAEAQAEASKVVREATERAAAAGKDVKAAAEAEA
                     AKARETAMAEAELERTRILGDLRGQVSALAIAAANKLVGEALDEKKQHALIDEFFSGV
                     KAGKVVVVDGEFKGDAAEVTSALPLNNQEQDAVKKSFGAKEVSFKVNPAILGGLVVKI
                     GDKVLDGSVAGKLEGLRQTLK"
     gene            23067..24638
                     /locus_tag="PL000_05760"
     CDS             23067..24638
                     /locus_tag="PL000_05760"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNC88434.1"
                     /translation="MLLKKLFVDFPLSMPEVIPDVEISGIAIDSRAVKPGHLFVAMKG
                     GFADGHAYIQKAIENGASAVVGERELDGLSVPYLRLENPRQALTWLAAAFYNWPARKL
                     TVIGVTGTDGKTTTSNLLHKILLAAGIKAGMISTVNAVIGDEVLDTGFHVTTPDAHDV
                     QAYLARMVEAGLTHVVLETTSHGWSQHRVDACEFDIGVVTNITHEHMDEHGSYENYRA
                     AKARLFSSLEWTHPKPAGNPRLAVINRDDSKSFGFLDEFIKVNKVNYGLGKDADVCGM
                     DIEYNPAGIRFVATSKDFRVAASSRLVGAYNASNCLAALSAAVYGLGIKPEVAAQGIA
                     ALEGIPGRMERIDLGQNFTAIVDFAHTPNALKVSLEAGRTMTRGRVISIFGSAGLRDK
                     EKRRMMAETSAELADLTILTAEDPRTESLDGILEEMAAGAIGKGGREGETFWRVPDRG
                     EAIKFALRLAREGDIVLSCGKGHEQSMCFGKTEYLWDDRTAMRAALADFLGVDGPAMP
                     YLPSRDMNESDWLKG"
     gene            24658..25119
                     /locus_tag="PL000_05765"
     CDS             24658..25119
                     /locus_tag="PL000_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC88435.1"
                     /translation="MIHTLHREQIIPTSVEVLWDYFSAPQNLNDMTPPDMNFQIVMGG
                     DVKMYEGQIIEYRVEFIRGIRSLWLTEIAHVREKEYFVDEQRVGPYRFWYHEHKFEPV
                     AGGVKMTDHVTYAAPFGFLGDLVNAVWIRRRLDAIFDFRHRRIIELFGSVK"
     gene            25116..25712
                     /locus_tag="PL000_05770"
     CDS             25116..25712
                     /locus_tag="PL000_05770"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC88436.1"
                     /translation="MTKPWATPVVLQGKHVRLEPLSEVHVDGLAEIGVGQDFWDFMLY
                     GRMETKEDMRNWVKDIMGRGEKGTDLPFTVIHLDSGRVAGATRYLNIMPNDRGLEVGG
                     TWYGLDFQRTPVNTECKYLLLSHAFEVLKCIRVQIKTDSRNIRSQKAIERIGAVKEGV
                     LRNHMILPSGIYRHSVFYSIIDTEWEQVKKRLEAFLEG"
     gene            complement(25802..27256)
                     /locus_tag="PL000_05775"
     CDS             complement(25802..27256)
                     /locus_tag="PL000_05775"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNC88437.1"
                     /translation="MNLPKLIQGGMGVAISDWRLAKAVSNLGQLGVVSGTGISRVLAS
                     RLMDGDISGNVRRALSNFSLPEPVQQIIDRYYTPGGKSPQTPYKTPAAYTIKPPKFLD
                     QVTAIANYVEVFLAKEGHNGLVGINLLEKVQMPTLASLYGAMLAGVDVVLMGAGIPTQ
                     IAGILDKLSNHEPVSYRLDVHGAGKDDDHRIHFDPEAIFPGIAKLVGKLKRPKFLPII
                     SSVVLAQALIKRSEGSVDGFVIEGPTAGGHNAPPRGTVNYNEKGEPIYGEKDAVDLEK
                     MKALGLPFWLAGGYGNPKQVQSALEAGAAGVQVGTAFSLCEESGMRPDLKQRLLEKVR
                     AEEAQVFTSPLVSPTGFPFKVAQLEGTLSDPEAYSARNRICDLGFLRILYKREDGSVG
                     YRCASEPVDVFVKKGGNLEDTVNRSCLCNNLTATAGFPQVRKDGYVEEPIITSGDDLV
                     AVGQFLKEGKTSYTAEDVVRYLLGPFMNVGQATA"
     gene            complement(27351..27770)
                     /locus_tag="PL000_05780"
     CDS             complement(27351..27770)
                     /locus_tag="PL000_05780"
                     /inference="COORDINATES: protein motif:HMM:NF026937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm63 family immunity protein"
                     /protein_id="HNC88438.1"
                     /translation="MFEPSFEKIQAELREYCRRMDPAYAHVKIQTIRGDDGTSHLEIR
                     DNSYHYIATERGLILSEKQTSDKNELLYWLVDEIAWSKACAYEFKHRVKGQSFRRLLF
                     AKNIEYLMMVKPAWAERKQKEFEAILAKHPYDDAAEG"
     gene            complement(27798..29051)
                     /locus_tag="PL000_05785"
     CDS             complement(27798..29051)
                     /locus_tag="PL000_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88439.1"
                     /translation="MASKLKILFNQYPRQFWLMVAGIVISTAGASMIWPFLLIYAGGK
                     LNLPLSTVATLISINAGTGLLASFLAGTLTDKIGRKAVMVFSLVVNGIAYLFLMQAET
                     YPQFIALMIFIGLCNPLYQVGADAMMADIIPTEQRTDAFAINRIAHNAAFALGPAIGG
                     FLAASSYDLAFYGAAVGFFAHSLLMFLFAHETLHKTVGATNGHSLADQPSVMEGFVRV
                     FRDGDFMAFVVLTGIGLIAPTMVWILMPVYAKTNFSLSEALYGWIPTTNAVMCVFIQY
                     AVTRITRHYKTLPVAAAGMFIYAISVGSVALMSGFWGFWLSMVMLTFGELTLVPTASK
                     YVADTAPADLRGRYMSVYWLAWGLARTLSPLIGGFINDSISPRAIWIGGLLIGMTSTA
                     GLLILNKRTLAQAALRPQPKPDSAS"
     gene            complement(29166..30860)
                     /locus_tag="PL000_05790"
     CDS             complement(29166..30860)
                     /locus_tag="PL000_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88440.1"
                     /translation="MNDLKDKPSFFSNPRLRITALVIILAALLLYYIDVFRTAGPTTF
                     DDAYMFIRYADNFIHGYGFAWNPDGVQTYGATSILYLGLVIIARIIFQGLGSGKLLVA
                     LSAGLGLPAIVLTAYTVSRYAESKLLKSSFLWLTVLLVGYLVTAPVYLFHMKSGMDTT
                     LSFLCNALLIFSTLGWVYNENRYRLFSTLTVIAGYLAFLARPDNLLYTFFFPVLAILF
                     LAPEGRRKRLVHFLGWLGVLLALDTLVKFIVFSDPLPLPFYAKSSGFYEGYIGTHTWN
                     PIEYLFAFGTLALPFLVLIVLSSTKQTLKLLAVFLLPIALTFTYYFTVVQIMGFEARY
                     YFPALPYLIVLSILMLDRSLSSGDTEGESHGKRLMRVLILLLIVALFSNSTLKTITSD
                     AYKNSQIAATKLYSPHTEYTTPAAKELPERGTWPMVLAVAKFSKSLPEGIIFAQSEYG
                     FTGAESPHLYIVDIIGLHDSYFAHNGFSVDEFLRRKPDLIWFPHFDYTKITAEIIDSQ
                     TFWEQYEFYPGAFDFGLAIRKDSLNYQAIHKAIQPVWREVYETRQMEDYLATPISK"
     gene            complement(30973..33231)
                     /locus_tag="PL000_05795"
     CDS             complement(30973..33231)
                     /locus_tag="PL000_05795"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC88441.1"
                     /translation="MKPESKRYSAVVGTRTFTFETGKLAAQAGGAVTFGVEDSIVFAA
                     ATMGGVRDGIDFFPLSVDFEERMYAGGKIPGSFFRREGRAATDAILTGRLTDRPLRPL
                     FAHGMRNEVQVIMYTFSSDGVNPLDILAINAASAAIMISDIPWGGPVGAVRVGRINGE
                     FVLNPTFDEMNASDLDLRIAGTKDAILMVECGANEIPEDVMVEALELGHKSIQPIIDL
                     QLQMQAEIGKPKREATIVTANEDLQKKVLDAVNARMNELLDKPLSKAEFYGGMDELQT
                     AIIAEFCVAGDENAPSEKDVKSAFSEAEHKIVRERILSMGKRPDGRTPTEIRPIWCEV
                     GLFPRAHGTGLFTRGETQVLSFATLGTLGEAQELDNLSPEKSKRYMHHYSFPPFSTGE
                     VKPLRGQSRREVGHGALAERALEPVIPSEETFPYAIRVVSEALSSNGSTSMGSVCGST
                     LALMDAGVPIKAPVSGVAMGLITDETGRYKILTDIQGTEDHLGDMDFKVAGTAQGITA
                     LQMDIKISGLSAQMMKEALAQANTARMSIMEKMLAVLDAPRAELKPHAPRIITVKIPV
                     DKIGALIGPGGKNIRALQEETGTKIDIEEDGTVYIASTDSVGAKIAQERVEGLGESVI
                     VGNIYTGKVVRIEAFGAFVNLLPGVDGLVHISQLASERVNTVEDVCVLGDELTVMVTD
                     IDPQGKIRLSRQAVLEGWSAEEAREKDKGGRGGGGGGRGGDRGGRGGDRGGRGGDRGG
                     DRRGGGDRGGRR"
     gene            complement(33408..33680)
                     /gene="rpsO"
                     /locus_tag="PL000_05800"
     CDS             complement(33408..33680)
                     /gene="rpsO"
                     /locus_tag="PL000_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC88442.1"
                     /translation="MPLAKEVKTKAIEDFHRHDKDTGSPEVQIAILTNRINQLTEHLR
                     SNKQDQSCRRGLLKLVGQRRRLLTYLRSSSYQSYLSVTDRLQLRRK"
     gene            complement(33870..34652)
                     /locus_tag="PL000_05805"
     CDS             complement(33870..34652)
                     /locus_tag="PL000_05805"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC88443.1"
                     /translation="MKELKFLFAIWKTNLQSAMEYRAAFLTQAIGMMVNNFIYFAIWI
                     IFFDRFKDVRGWGVNDMYVTFGVLASAFGLVSLFFGNAFNLSDIIAKGRLDYYLSLPR
                     PVLLHTIASRTIASGMGDFLYGFVSYGLSGYFTWDGLLRYVLAMLLAATVFAAFLILT
                     QSLAFWFGMMTNFSNLVLNAMLTFGIYPITLFDSYAKLILFTLIPAALMGAIPAEFIR
                     AFTWQTLGELLLGAIGFLTLAVFTFRMGLRRYESGSAIQIEV"
     gene            complement(34694..35494)
                     /locus_tag="PL000_05810"
     CDS             complement(34694..35494)
                     /locus_tag="PL000_05810"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC88444.1"
                     /translation="MKSISKYWSIFQITLLNSLAYPGELVGRSLMIIPFMWIFYQLWK
                     VTFSAAGAVDGMINGLTLRDTLWYLMLAETIELGRPPIARTMSENVKDGSIAYILNKP
                     YDFLLYQFSTAMGETIFRAMLNAVFGGLTIWWLVGTPNHPEGFLIALPAILGAWVLHF
                     AINAMIGLSAFVVEDVSAFTWIYQKLAFIFGGMLIPLDFYPQWLQNLAKLLPFSSAIY
                     GPARLFVTPTYDLFVSVMTLQVTWIVALVVALTIVYRRGLTQLTVNGG"
     gene            complement(35579..36403)
                     /locus_tag="PL000_05815"
     CDS             complement(35579..36403)
                     /locus_tag="PL000_05815"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HNC88445.1"
                     /translation="MDKNFWHTIKENKYAFPQGHDLTALTDELFSYIPSTDPDLRDGI
                     GYEIFANWIETEPYQAEQLRGYLKRLLENLQVGLGQQDDDSVFGRAFSVLYLAEVIHR
                     DNQQPYLESHEVKETLTRVLAYLAAERDPRGYVPVKGWAHALAHTADMLMVFARSPHL
                     GATELEHILSAVSEKMRAAKNWIYIHGEDDRLARAIVTALAREELSLEQVKAWFENLT
                     TDWKGAWDHEEQSRAYLNARNLLRAIHLRILQMKELPRQEELSALALEAAASMRPF"
     gene            complement(36418..37407)
                     /locus_tag="PL000_05820"
     CDS             complement(36418..37407)
                     /locus_tag="PL000_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC88446.1"
                     /translation="MLSIDVSNLQKTFQTKRKAAGLSGSIRSLFKPEYSLVEAVHNLS
                     FQMGTGELLGFIGPNGAGKSTTIKMLTGILHPSGGEAKVLGFTPWVDRQKLAYHIGTV
                     FGQRPQLWYHLPAIDTFMLFGKIYELDDREIKKRIDFLTEAFEIKDLLDVPVRKLSLG
                     QRMRCEVAASLLHRPKLLLLDEPSIGLDVVAKQHIRDAIKRMNQEEGVGVLLTSHDAG
                     DLEALCKRVIIVNHGQIVYEDKVSNLKRKFLTTKLVEVRYALEVSKDFAIQGVETLKV
                     GHYGVKLKFDTTQTPVDTVISHLSSAGDIVDITISDPPLEEVISKIYQEAGNA"
     gene            37466..38119
                     /locus_tag="PL000_05825"
     CDS             37466..38119
                     /locus_tag="PL000_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88447.1"
                     /translation="MRRLSWVIVLAFLLQACAGFSLNPPTSTPTITSTPTITYTPTIT
                     PTITITPTRTALPTIVRIPTWDPNQPTPTFMAIPIFIGSVTATPIVSPTPTRPGIGFV
                     SVDVSEQKIFWGSCKHNKAKIIAKVQNPDEVFSVVIFVRVKALLKEDYTPWTTGDVMT
                     KHGDGTFSYTLVGSEIEGHNHYRDSWVYFQLVATNEEGEIVGRTMIYTNSLSLSPCK"
     gene            38135..38833
                     /locus_tag="PL000_05830"
     CDS             38135..38833
                     /locus_tag="PL000_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88448.1"
                     /translation="MKKFYALSLCTIMLLSCSLNLLAPSISSTPVPTLTNTPTDLPSA
                     TPTVPTPTFTPTPPLVGLRTHTLTPTITATSTQPTETSIFLATLDTPTPSVEMKGFVS
                     VNVSTKEFFKDAECQPTSVKFVAQVADSAGTAFVVLFVRFKSKQTGTTSEWTSIGMST
                     IGAGTYTYELKPEEMKGVDSFKNPWVQYQFVATNADANEIGRTGIFSERLSLLECEPT
                     PSPSPSPEPTVLRP"
     gene            38830..39567
                     /locus_tag="PL000_05835"
     CDS             38830..39567
                     /locus_tag="PL000_05835"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNC88449.1"
                     /translation="MMEFPDDLIRFLTKATRVSVLTGAGASQESGLRTFRDAQSGLWA
                     QYKPQDLASPEAFARDPKLVWDWYAWRREAIKGVRPNAGHYALVDMEKKVEVFTLITQ
                     NVDGLHRMAGSSNVLELHGNILKVRCSECRTFTEVWGDDSESVPLCDKCGGMLRPDVV
                     WFGEALPREELEAAVNAARSCQVFFSIGTSGVVQPAAALAHAARNKGAVVVEVNAEPT
                     PLTPKTDFAFHGKSGEILPELVRAVWG"
     gene            complement(39583..>39873)
                     /locus_tag="PL000_05840"
     CDS             complement(39583..>39873)
                     /locus_tag="PL000_05840"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HNC88450.1"
                     /translation="MTIKFKGKIFHWRGPAPFLFVAVPEKPSRDLKAISSSVTYGWGV
                     IPVQVRIGSTEWKTSLFPKDGRYLVPIRMSVQKSENIGVDDNVTIYLEVAWP"
BASE COUNT         8666 a         9708 c        10948 g        10551 t
ORIGIN      
        1 tgcagggcgt tgaggcgcag acccgtatcc gcgaggcgct ggaagggctc gcgcagattg
       61 gactcgatgt tgagatgcgc gaataaccgt tccttcggta acttgcgttg cgccccgtcc
      121 ttcacccaat attgcaacag tttgcgattg tcttcgacct tgttgagcgc gttgcggcgc
      181 aagcccgcat cgcggatgtt ggtgatgcct tccagcaaga aatcgtaagt gcggtcgagg
      241 gcttcgcgtg cttcttttgt tttcccgttg gcgatcaatg cctgcgcatg ccaccaccag
      301 atgtattgcg aaccagtgcc ttcgggtctg gcaaagtttt gggcgcggtg cagggctgtg
      361 gctttgcttg tggctttgag cgcggcggcg gggttgctcg cggcgagata gtatgttgcc
      421 aacgcggtga gggtggtatg ctcaccgctg atctgtgact ctttgatcag tttcaacgca
      481 gatttctgat ggcgaatagc agtcaggatg tcgcctgtgg caaatgccag gtctgcccgt
      541 tggttgaaaa gacctgcttc caaacttggg tcgccgaggt cgggaacgag tgtttccaac
      601 tcccgaaggt gcagacgcgc cgcctccacc tcaccgaaaa ttgtttctgt cccgatgaga
      661 tttcccagcc cgacgatcaa agcaattttt gcccccatgg cgcgaccttg tgcaatggat
      721 tctcttcgca aacggcggac atgcgcatac aaaccaagat aactataagt attcgccaag
      781 ttggcgatga cagcgttttg gcgatcgaca tagcccacgg tttcaaaagc gctctttgcc
      841 tgttgatagt gttggatttg ttcggcgata tctacatcgg tatttgccaa ctgaagcagg
      901 gcattgccaa taccatacga gtcgcccgcc tgcttgcata gttccagtgc ggtctgcgag
      961 gcgcggcgcg attcttcggc gcggtctgag cgaaagaaaa cacccgataa cacccaatac
     1021 gcgcgtcccg ttcctgatgt gtcgccaagt tcttgatagg cgtcaatagc tttttgagct
     1081 gtttcaagcg cggcgtcgag atgtcttgcg cgccattgcg cttcggcgag gcggtagagg
     1141 ctttcggcgc gtaacgcggt aggggcgggg tctccccgcc ctgggcgcgg aaaccgcgcc
     1201 cctacggtgg attttgccgt tttgacagcg cccttcaagt cacctgtccg catttggacg
     1261 agggcgaggc gattcaaggc ttgaaccttc aaacctgcaa cctttgaacc cattttcatc
     1321 atcgccttgg cgtctttcat cgccaggtcg agtttgccga tggcgatgta ggattccgcg
     1381 cgcaggtcga gcagggacat catcgtaggg gcgcggtttc cgcgcccttt gccagggcgg
     1441 ggagaccccg cccctacgtt ttccaatgcc tctgtcgctg aatcaattgc cgccgcgtgt
     1501 tgacccgtcc acgccagttg gcggatgtga gtcagggcct ggtcatctgt attttttgtt
     1561 cttttgtttt ttgtgatgtc tgattttttc ttcaccataa aaagcctatt cacccaggta
     1621 ttttttcaac atcttgaaca acaaatcatc attgacaggc ttggtcaggt aatcggtgca
     1681 gcctgcatct tttgcttttt gttcgtggcc gtccatggca tgagccgaaa ggccgataat
     1741 gggtaaattg ggcagggtct tgcggatggt gcgtgtggct tcgtacccgt ccagaatggg
     1801 cagggagata tccatgagga tcaggtcggg ggtgtgttcg gtggccattg aaacgccctc
     1861 gataccgtct ctggcgacga gcaggttata gtcatcttcg agtaattgag tgatgaggtc
     1921 aatgttcaat tcggtgtctt ccacaacaag aatcgttttc ataaaaggtc tcctaataac
     1981 gtcagttttt cttgggttga aattgaatgg gcagggtgag cgtaaaaacg gatcccttgc
     2041 cgagctcgct ggttgcggtc aattctccgc ccagcaggtt tgcaaggttg cggctgatgg
     2101 tgaggccgag acccgtgccg ccatattgac gggtggtgct gttatcggcc tgttggaatt
     2161 ctccgaacac aaatcccagc gcttcttcgc tgatgccgat gccagtgtca ctgaccgaga
     2221 tgcggaagca gggtacggga acttccgctg aggcatccac tttttcgacg gccagaatga
     2281 cctgtccttc gtgtgtgaac tttgcggcat tgctgagcaa atggagcatg atctgtttga
     2341 tcttttcctg atcagagtgg atgagggtga gttgttcgct ggcgcgcctg tcgaatttca
     2401 cggcaggttt catggaaggc agggcaatgt tgtagcattg ttcgatgagt gcgttgatgt
     2461 tgaagttggc ggcatgcacc tccatgcggc ctgcttcgat cttggcgata tcgagcacgg
     2521 cattgatcaa ccccagcaga tgttctccgc tggtgagaac cttatcgagg ttttcgatct
     2581 gtttttcggg gagcaccccc tcggattttt tccgtacaat gcgggtgaat ccgataatgg
     2641 cattgagcgg ggtgcgtagt tcgtggctca tgttggcgag gaaggcactc ttggcggcgc
     2701 tggcggcgtc ggcggcggcg cgggcttcct gtgcctgttg atacagacgg gctttttcaa
     2761 gtgccacgct catgctgctc gccagtgtgg tcagcaagcg cacatcggca ttgttgaagg
     2821 cgttttcgcg gtcaatgtta tcgaggccga tcacgccacg cacttcgtcg ccatccatga
     2881 tcggtacgcg caggagcgat ttggtcggtt ttgtgcctgg cacggtttgt gatgtaatgc
     2941 cgatggaggc cagccgttcc ttgaagtttt cgttgatcag gatcggccgt ttgttttgaa
     3001 tcagaaattg tgaaagcggg ggaattgcgg catcggggat gtcgtggcgt tgtccttgtt
     3061 cgagcatgta gtgataatgg cgcgtgcctt ttttcaaatc gaacgagatg atggaaatgg
     3121 cctgcgaatc gaacaggctg cgcaattgct ctccaatcaa ctcgtaaatg gcgttgacgt
     3181 ccaattgaga tgccagcccg gcctgcacac tgttgaggat ggcgagttca tccacgcggg
     3241 catgaagttc agcggttctt tcctggacct tgagctcgag tccttgtgcc caattcacat
     3301 tatccaaggc gacggcggct tgattcgcca acatgccgag catgtcacgg tccacttcgg
     3361 tgaaggtgcc gtagagtgaa tccatgtcgg catagatgta gccgatgatc tggttttgcg
     3421 cgatgagcgg ggcgatgatc ctgctcaaag tgctgtcact gtcgttcgca ggcagaatca
     3481 gttgcacggt ccgcgtgagg cgcgcctggg tgaggtaggg gtcgatcagg ttgagcactt
     3541 cctgcacatt ctcgccgcgc ggaagaatcg actcggcagc ttcgcaaacc ccgttctttt
     3601 cgaggataaa cagcacccgc tctccgccgc tcaactcggt ggcttcctcc acaatgaagg
     3661 tgtgcatttc actcgtggtg tgcagggcgt tcaatcgcag ggcggtatcg gtcagccgtt
     3721 tgaaaggctc gcgtgtgttc gattcgagat actgatgggc gaagatcttt ttcttcgaaa
     3781 gtttacgcct gccgcttgca ttcagattgt cgctgatcca aaacttcagg agctcgcgat
     3841 tctctttgac cttgttgagg cagttgcggc gcagtccttc atcacgcacg tttgcgatgg
     3901 cctcaagtaa aaattcatgc gcccgtgcca gggccttgcg tgcttcaacc tctttgccgt
     3961 tggcgaataa tgcccgggca tgggtccacc agatttcgtg agaggagtag ctgtcgggta
     4021 gtttgtaatc ctgactgcgg tggagttcag tggctgcggt ggtggcggca agggcggcaa
     4081 cagggtcgtt gtttttcagg tgtgcaattg caaggtagct gagatggaca ttttcattcc
     4141 cgatctcggc ttcacgtgcg gtttgtactc catcttgaaa acattgaacg gcttgttggg
     4201 tttttccttc tgccagcagc atgagaccct gatttgtgtt gaaactggcg ctcaaacgcg
     4261 ggtcaccaaa ttcagcagcc agcctgccca tttcgtttaa atgttcgcgg gctgattcga
     4321 tcagtcccat tctcaattca gtgtcaaaca ggttttcaga ccagtacgcc atgccgatct
     4381 tggcaccgat cgccttgtct atttcaaagg ctttgcgggt caggcggcgg gcatgtgtat
     4441 aaaggccaag ggctgcgtac acaaaaccca aattcccggt agaggtggat tgacgttcca
     4501 gatgccccac tgcttcaaaa gcctgaatgg cctgctgata gtgatgaata cgctcggcga
     4561 gatcggggtc ggtggaactt aatccattca aggcgttgcc aatcccaagt tggtcgttaa
     4621 cctgctggca caactccagt gccgtttgag cggcatgacg ggatttttga gcaaacccca
     4681 gaacattata ggagggtgcc atgatgtaac tggcacggcc tgccccggag agatcgcctt
     4741 cagccagaaa tatttccatg gctttttgcg cttcggtaac tgccgcctca aattggctga
     4801 catttttata ggcctcgctc attcggaagt ggctcattgc cagcaggcgc ttgttgcgga
     4861 ttgcatttgt cgaagcgata tccagagcca gcgtggctgt tttgatggcg tcctgcacat
     4921 cgcctgagtg gatctgaacg agggttttac agttgagcgt ctgtaccttc aattccccct
     4981 ggtcggcttc ggtcaactcc atcatggtat ctgcatcttt ggcagccagg tcaaacctgc
     5041 cttgcgcaag ctgcgattcg acgcgttgat tgagcagacc aatcctggtt gcaagtgaat
     5101 gttcgtcttt gcttaattca agtgaagtga gtttgatcgc ctgggtatgt tgccccatcc
     5161 aggcaaggtt atgggcgagt gtgatgggtg aggaggggtc ggtaacaggg gcggatggag
     5221 gatgactttt cagcctttgc ccactttcag tcgcctgcat tttcgccccc tttttaacag
     5281 cctttcttac gggtgatgct ttttttgcca tgcccgtttc tcctcaacct ctggtttgat
     5341 taaatggaag tttatcgggc ggagcacctt aagtcaatgg gagaagttgg gttggatatg
     5401 tctgcaatgt gaatttaatc cgatcttaac caaccgacaa ctggttctta aatcccgact
     5461 cttatactac ccgatcatga catttcaaaa ccgtgttctc tggcttatgt atattcttct
     5521 ggtccttgca caatgggtgt gggctttgtt tctacggcga agggagcggg ttgagtatct
     5581 gaaatatctt ttacaagatt ctcttgcata attccctggt cacctgattg aatcattctt
     5641 cacaagtctg ctgcaaaacg gttgcattat attttgcggg gagtataatg ggcgaaatct
     5701 attccgcacc acctctgcaa gttatcccat ttttcaagga tttgcatgtg actaccatat
     5761 tttttgctac agatatacat ggttcggata tttgctggag caagtttctt aatgcgggga
     5821 aattttacgg cgccgataaa ctggtactcg gcggcgatat gacgggcaag gcggttgtcc
     5881 cgtttgtgca tcagggtggc aaaaattacc gcatcacttt gcttgaacag gtgtttgaaa
     5941 caacaaatga tgaagagctt gcggatatga ccaaacgtgt ccgcagtcgc ggctattatc
     6001 catatctgac caaccccgat gaaattgcgg aattggaaaa agaccctgac cgtgtcagca
     6061 agatcttttt gcagcaggta ctcaaggttg tgcaacaatg gatggacctt gccgacaaaa
     6121 agttggatgg caccgacatg atggtctatt gctcgcctgg caatgatgac atggacgaag
     6181 tggacgagat tatccgttca agcaaacggg tgatgctggt cgaaggtctg gtctcccagt
     6241 tggatgaaaa ccacgagatg atcgcctcgg gctggagcaa tatcacaccc tggaagacgc
     6301 accgtgagga gaacgaggac cagctggcag tcaggtatga agcgatgtac tcgaggctca
     6361 aagacccgcg caattccatc ttcaatatcc acgtgccgcc tcataaatca ggtctggatg
     6421 aagcgcctga actggacgaa aatctgcgcc ctgtgctggc tggaaactca atgatccccg
     6481 ttgggtccac tgctttgcga aaggccatag aaaaggttca gccgttattg agtttgcatg
     6541 gtcacatcca tgagggccgc ggcgcaacaa ggatcggcaa gacactttgc atcaatccag
     6601 gttccatgta tgagcaggga accctgcttg gcgcaattgt caagttagga aagaacaaga
     6661 tcgagaatta cgttctgaca acaggttaac cgtaccgcaa aatttatttg cagtactaat
     6721 aaaaccaaag aggagagagt tatgagcaac ttgtttgtcc gtaaggcgac tggcatggtt
     6781 cggtcctggt cagtcttcga cgcatttatt tatgcgttct tttccatcaa ttttgtgacc
     6841 ctggggtttt atgccttcag ccagatgtac tactttggcg gcggcatgat caatgctctc
     6901 atcatcagcg ccatcttcct cgtatttgag atcatcgtct acgcttcgtt gatcgcggtc
     6961 atgccccgtt caggcggtga ctatgtctgg atgacgcgta ttttcggcgg cagcattggc
     7021 ttcatccttg ccattacggg ttggtggttc accctgtggc tgtggacccc tgtttatggc
     7081 gatatgttcc gccaggtggt attggtgccc atcatgggtg tgcttggaat gagagatact
     7141 gcggtgtggt tcgcgggtac tggaacaccc ctctttgtca gttcactcat tacccttgcc
     7201 ttcgtttccc tcgtcatcat ccttggcatg aaaacctatg cacgcattca gaagttttcc
     7261 ttctatgcgg gtgtgcttgg tttgttgatt gtcattgtcc tgctttttac aggttccccc
     7321 gataaattca aagctggctt tgagaagaat gccgccgcca ttttcggttc ccagcccggt
     7381 gtgtatgatg ccaccgtgca gacaggcacc gatgccggcg cagccacccc gcttacgggc
     7441 ggctctttca gcctcatcat ggtgaccctt ccgtggttgg ttttcttcaa cttgtggccg
     7501 aattggggcg caaccttgta cggcgaagtt cgcggcgcca cggattacaa gcgtaacttc
     7561 tcaggcatgg cttgggcgct tggcacagtg accgtgcttg gtattgtctt cttcctcggc
     7621 gtggcgaaga ccatcggctg ggatttttat atgcaagcca atggctcctg gtggaattac
     7681 tattggggct acgttaccga tgtgcccccg ttgcccgtat ggccttaccc tgccatgctg
     7741 gccacattcc tcactgaaag ccgcgccttg cagctcatcg taatggtgct gatgagcttc
     7801 tggtggttcg gttgggctgg taccttgttc ctttcctcta ctcgcgtggt gttcgctgca
     7861 gcgtttgacc gcctgctccc cgaaaaagta gctgaattga atgagcgcgg tacccccgtc
     7921 aacgccatgt tgttgatggt tatccctgca gtgatcgttt cctacatgta tgcctataac
     7981 gttggcggtt ttatttccgt tacccttgcc gctactcagg ccattgcgat catgtacttt
     8041 ggaacggcca tcgcagctat tgttctgccc tataagaaga aagacctctt tgaggcttcg
     8101 ccaatcgctc agatgaaggt tgctggcatt ccgctcatca ccattgcagg tgtgatcttt
     8161 gccagtttcc ttggcttcct gctcgtggag tggttcttcg acccatggct caacacgggt
     8221 ggtgaaggcg cgggcctgta tggcatcagc ttcaagaata cgaactccgt catcttcctg
     8281 ctggttacct acggcctggc tgccgccatt tactacgggt tcaagtccta ccgtaagaag
     8341 agcggcattg atatggacaa ggtccagacc gaaattcctg tcgagtaagt aatacaaact
     8401 gggtgacagc cgccttgcat gggtggctgt cacctttttg atttccaatg ggagtgcaga
     8461 atcatgccag tgatagatga agtggtctcc aaaatggaag catggaaggg gagagatgta
     8521 tccatcgaac acctttcagg cggattaacc aacaccaatt acaaggtcca tgtagatggc
     8581 cgtccgtttt ttatccgcgt gcctggggaa ggcaccgaat tactggccat tgaccgcaac
     8641 aacgagtatc acaacagcag agccgcggcc aatgcgggtg tcgcaccgca gatcctgcat
     8701 cacttcccgg aatacagtgt gatggtgttg gaattcctaa atggtaagac catgtcgaag
     8761 gattccctaa atcagaaggg gatgcctgca cgcatggccc agtccatcaa gaaactccat
     8821 gcgggcgaac gcttcttgac cgacttcaac atgttccgcc ttacggaata ttatctgcgc
     8881 ctgtgcaagg aacgcgatat caaaatccct gatgggtatt tggacaggct ggagaccgtt
     8941 gccctcattg aaaaagcgat gtcggtgaat caacttgcga cggttccgtg taacaatgac
     9001 ctgcttgcag aaaactatat agacgatggc aatcaacttt ggctgatcga ctacgaatat
     9061 agcggcaata acgacccgac ctttgagctc ggcaatacct gtcaggagat gcaattcaac
     9121 gacgagcaga tcagggaggt gtgtgctgca tactttggcg atgtctctcc cgccatgatc
     9181 gcacggatga agctgaatat gatcatgtct gatgtgggtt ggggattgtg ggcagccatt
     9241 caggagaaaa tatccaccat cgagtttgat ttttggggtt gggctttgga gcgttggggc
     9301 agggcccagg agaaaatgga ctcgcctgat tttgatattt ggctcaagga tgtgcgacag
     9361 taacaagata aacacacagg cgcgcaagcg cggaagcatg tatacttaat taacctgtgt
     9421 tcgcgtttcc aattttcaaa ggatagaatc acttggatat catacaagaa atgcaccgcc
     9481 tcattgatgc ggccaatgga aagaatattc aactgcgtgc catcggtggg ctggccgtgc
     9541 agtctcacaa caagaagaat caccccttgt tcctcaggga gtttgccgat cttgattttg
     9601 tggtcgccaa aaaacagcgc cgtgaatttg aggcattcat gcccgaagta ggatacgccc
     9661 ctcatcggca gttcaatgtg ttgaatgggg atcaacggca gatctattac cacggtgaga
     9721 cggatatgaa gatcgatatc ttcgtgggcg actttatgat gtgccataaa atcccgcttg
     9781 aaaatcgctt aaaagccgac ccggtcacca ttccattggc agagctgttg ctctcgaaag
     9841 cccagatcgt ccaccttaac cgcaaagacg ctttggacat tgcctccctt ctgctgaata
     9901 tcgaagtggg tactggcaac gataacatca tcaacttggc cgtcattgca caattatgtg
     9961 gcagtgactg ggggttgtat aagactacgt ccataaacct ggagcgggtc aaggaagtgg
    10021 tctctgcgga tgatgttgcc ctgacctctg atgaaaaatc attgatcgtt tctcgtgtgg
    10081 ataaagtgct tgatgctttt gccgccatgc caaagactct ggcctggcag atgcgtgaca
    10141 aggtggggac acgtgtacgc tggtatgagg aggttgagga ggtcgcacgg tgagtaagca
    10201 cctgcgtttg ttctttgccg tcctcatcct tacactctcc atttcgttgc tggtttgggg
    10261 atacatgccg aatccgcacg aaacacggat tcaatggatc gcgccgacag aaatgcagct
    10321 tccatgaaag aggtactcaa gccgctgtat cggctggtcg tttttcctgt ttttatggtc
    10381 gtattcattt cccttgcttg tgttggcaat ggaaacgctc cgtcctctgc tagtgagccg
    10441 accgcgatct ctgcacttgc tacgcaaact accaacgaag tttttcctga acctgcacca
    10501 gccatccccg aaacccgccg tcttacgctt gaattcccgc cgaaaatgcg ggcaggtgtg
    10561 gaaggtgata ttgtccgctt gactttagag gtggatgacc tgggaaacat cactccaacc
    10621 gctcaatttg agggaaatgt agtgacgggt gaggtgattg agattcccga tttatatgaa
    10681 acgcataatg tcaccgctga agcccggctt gatatggctg ggatgcagat tcagccctca
    10741 ggcgcgattt acgagccgct aaaacgcgga caatctgcca catttttttg gagtattcgc
    10801 ccgcaggaca caggggttta tcgcggtacg gtctggctgc atttgaattt tacggacatc
    10861 tcaacgggag aacaaagccg aattgcagta tcagcacaca cggtcgaaat tgaagcggtg
    10921 gattttttcg ggctgtctgt caatgtggcg cgaacaaccg gggtggtcgg ttctgtggtc
    10981 ggtggcatcc tcggattccc gtttttagag gatattatca agttcctgct gaagtttcgg
    11041 agaaaaaaac agtctccatt ggcaaattaa atatatgacg aaaatcacct aaaatattga
    11101 catgcagtta tagggggtga tttgttctat atggtacaat tttcccatac aagattttcg
    11161 ctatggagca cacaaatgaa tgaatggctt tacgttggat tgttttttat tgttggtctg
    11221 atcatccccg tgggcgcaat tggtgttggc tggcttttga gccccaagaa gcccaatccc
    11281 atcaagcaat caacctatga atgcggtatt gaacctgtgg gcgaaggttg ggtgcagttc
    11341 aaggctcagt attatatttt tgcactggtc ttcctcgtgt tcgatgtgga agcagtgttt
    11401 ctttttccct gggccgtgaa gttgaatcag ttggggttgt tcgcagtggt cgaaggtatt
    11461 attttcattc tcattctcgt tgcaggcctg gtttatacct ggcgcaaggg gatgttggag
    11521 tgggcttagt tttcaacaca ataacgagaa ggctgaacgg atttttcctt tcagcctttt
    11581 ctttgtgtaa agcaggtaaa ttttcgtaga gcgaactggc acttcgctct acagatttac
    11641 aggagtgact tatgaatttt tggaatgacc ctcttaaaat agccgcagat tggctgacag
    11701 gtatctttgt tgggtggggc atgaatgaca ccgccgccca tgtactggtt gcctttctcg
    11761 gcgtgttgat tctcatctcc attttgatgg tggtggacat tttccttgtg tggatcgaac
    11821 gcaaggtggt ggcgcgtttt caggatcgca ttggcccgaa tcgccttgga ccttttggcc
    11881 tgatccagcc attcgcagac atcatcaagc tgatgatcaa ggaagacacc acgcccgatg
    11941 gcgcggacaa ggttgtgtac aaccttgccc caatgctttc gatgatgtcg gtgttgatcc
    12001 tgtgggcggt ggttccgctt gcgcctgtga tgctcggcgt ggacctgaat gcgggtgctt
    12061 tgttcatcat tgccgcaggt tccattggca cgctgtccat cattatggcg gggtggtcgt
    12121 cgaacaacaa gttctcggtc atcggcgcat tccgtcaggt ggctgtgatg gtttcgtttg
    12181 aaatcccgat gttggcgaca ttgctcatcc cgaccatctt cgctggttcg atggggatga
    12241 acgcgatcat ccagggacag gatatctggt ttgtgctgct ttctccgctg gcggccttga
    12301 tctttttgat cgcggccatt gcagagttgg gacgtgctcc tttcgacctt gctgaaggcg
    12361 aatcggaact ggtctctggt tacaacattg aatattctgg catgaaattt ggcatgttct
    12421 acgcaggcga gcttttgcat gcctttacgt ttggtggttt ctgggcaatt ttcttctttg
    12481 gtggttatcg cttcttcggt ttggaaaagg tcagtccttt ccttgcgatc gcgatcatcc
    12541 tgttgaaggc gctcgttgga tactggatca tcatgtgggt caaatacaca gtgatgcgta
    12601 tccgtattga ccagatgctg gctttcaatt ggaaattcct cacccctgtt gcgtttgtgc
    12661 tggtgatggt aacagccctg atgaatgcgc ttctgcgcga ggcttcaagc gggctttata
    12721 tcacgggcat gatcctctcg aacatcattg tcggctgggt ctcgcttgag atcgtccgtt
    12781 ccatgagcag gaaagagcgc gagcgggtgg aaggtgtaaa gcaggtggtg gaggctcacc
    12841 actaaatcct tcgcatgagc gcggttttga tgatcgggtc caaaaacggg acccttcgct
    12901 cagtgcgaaa caggagattt tatgacagca gaacaaatca tttttctcat tgttgcgggc
    12961 gtcaccctgg cttcggcttt gttggtggtg accacacgca accttgtcca tgcggcgttg
    13021 tggctggtgg ccacactttt tggtgtggcg atcatttacg ccattctcaa cgcaggcttc
    13081 atcgcggtgg tgcaggtggt tgtgtatatc ggcgccattg ccattctttt catttttgcg
    13141 gtcatgttga cccgcagaga gttgcgtgac aataccccgc agttgaacag gaattggtgg
    13201 tcaggtgcgc ttgtggcggt gttgaccttt gcaggcctcg cctcgctgct ttcgaggtgg
    13261 agcggcttct cgaagaccgc agcagccctc ccgtcaggtt ttgatgcggt ggccgagttg
    13321 ggcaaccagc ttgtttctcc cgatgcattt gtcctgccgt tcgaagtcgc atccgttctt
    13381 cttctcgcgg cgatggtggg cgcagtttat gttgcgttca cgcgcaaata ggaggctgaa
    13441 gagatgatcc cactttcctg gtatcttatt tttgcggcag cgttgtttag tgttggtttg
    13501 tttggcgcat tgtcgcgtaa aaatgccgtt gcaatcttgc tcggcattga gttgatgttg
    13561 aacgcgatca acgtcaacct tgtggctttc tggcgctatg gggatgtggc ggtgatggct
    13621 gggcaggttt tcgccgtcat cgtgtttgct gtggccgcag cggaagtttc cgtcggtctg
    13681 gctttggtca tttctgtgta tcgccgccgc aatacggtga tcgcagatga actcgatatg
    13741 ttgaagtggt agggacgtat tatgacacat gaatttgtga cgaatttggt ttggcttatt
    13801 cccttgccac ctgtgattgc ttttgcgttg atcgtgttat tcacgaacaa gagcaaggct
    13861 ttgagccata cagtggcgat cagtgcggcg ttcctctcct ggctgggtgc catgatcgtg
    13921 ttttggaatg cggtaaaggt tgagcatttt gcagaagaac cttttgcggc ttccgtcaac
    13981 tggctgccga caggtgaaac ttggttgaag gtcggtgtgt tgatcgaccc gctttctgcg
    14041 accattttgt tcttcgtggc atggactgtt ttgatgatct tcctgtacag tgttggttat
    14101 cataacttcg gccagcctgc aggcgaccac gaccacaagg gcttgccccc gcatggcgca
    14161 actgtgactg acgacctcgg ccacaagcac actgtgcttt caattgagcc gatgtactcg
    14221 cgcttctttg ccttcatcag cctgtttgcc ttcggtatgt tgaccctcgt ggtttccgat
    14281 aacatcctga ccctttttgt gggttgggag atcatgggct tgtgctcata tctgctcatc
    14341 ggcttctggt acggaaaaga atccgcgcgc aatgccgcca tcaaagcctt catgaccacc
    14401 cgtatcggcg atgtcttcat gcttttgggg atttcattcc tgtacagcgt aacgggtacg
    14461 ttgtccttcc gtgaaatctt caccgaagaa attctgcaca cccttgcttc tgttccgagc
    14521 ggtgtgtttg gcctctctgc ggcaggtttg atcggtttgc tactcttcat cggtacggta
    14581 ggcaaatcgg cgcagttccc gctgcacgtt tggctgcccg acgcgatgga aggcccgacc
    14641 cctgtcagcg ccatgatcca tgcggcgacg atggtttcgg cgggtgtgta tgcggtcatt
    14701 cgcatgttcc cgatcatcag cctcgatgaa aagacgatgg cggttgttgc tttcatcggt
    14761 gcatttaccg cgctcttcgc ggcgaccatt gctgtggcac agaatgacat caagcgcgtt
    14821 ctggcttact cgaccatttc acaacttggt ttcatgatcg cagcacttgg catccatgcc
    14881 tatgttgcgg ccgtcttcca tcttgtaacc catgccttct tcaaggcttt gctcttcctt
    14941 ggttctggct ctgtcattca cggcatggaa catggcgtga tgcacacagg caatcacaaa
    15001 gttgacccgc aggatatgtt caacatgggc ggcttgcgca agaaaatgcc catcacattc
    15061 tggaccttcc tgatcggcgg ctttgccctt tctggcttcc cgatcgtaac cgcaggtttc
    15121 tggtcgaaag atgaaatcct tgcggatgcc ttcggccatg gacacaccgt tattttcggc
    15181 accctcgcct ttgcagcttt cctgaccgcc ttctacacca tgcgccagat tacgctgacc
    15241 ttcctcgggg aaccccgcac gaaggaagcg gaacacgccc atgagactcc ctggacgatg
    15301 acaactccgc ttgtagtgct ctcggtcttt gctgtgacct acggctgggt cggcattccc
    15361 gagcatttcc cactcctcgg cggactcgtg ccaaactggt tccatgaatt tgtcggctct
    15421 acgttacatg aacaccctga agccctggag ttcagcatcg ttcctttgct cacctcgctc
    15481 ggtgttgctc ttggcggctt ggggctgggc tggctggttt atcgcaacgt caattctcct
    15541 gctgaagaca ggctgcagat tggtctgctc aagaacaaat actattttga cgagatttac
    15601 gatgttgtct tcgtcaagcc tgcctactgg cttgcagaga acgttatcta caaatttatg
    15661 gatcaaggtg tcattgacgg cattctccat ttctttgggc cagccacagg cggagttgga
    15721 agcacgatcc gcaattactt tgacattccc gttatcaatc atctgattgg tgatggttcc
    15781 gccgatgcga cctactggat cggcaagaac ctccgtccca ttcaaacagg gcgtattcag
    15841 caatatctca ttctctccct gatattgctt ttcgtggtgg gcggcctggt ctactacatt
    15901 ctcacactgg cgtaggagca tacgatggac tttctcaatt ctcatctcct tagcctgatc
    15961 ttgttcctcc cgaccgttgc ggcagtcatt atgctcttcc tgccgagtga agaagccaaa
    16021 ctccagcgct ggtttgcatt tacggccagc ctcattccgt ttggtctcac cctctatttg
    16081 tggaacgcgt ttgaccccaa cagaacaggc tttcaatttc aggaacaata tacgtggtac
    16141 gaagccatcg gctccaactt ccaccttggc gtggacggca tatctctttc gatggttttg
    16201 ctcaccaccc tccttacacc attggccatc ctcgcttcct tctcggtcag cgacaaggtc
    16261 aaggcgtatt tcatgctttt cctcttcctt gagacaggta tgctcggcgt cttcatggcg
    16321 cttgacctgc ttatcttctt tgtcttttgg gaaatcggcc tcgttccgat gtacttcctc
    16381 atcaatcagt gggggagtgc caatcggaat tatgcctcgc tcaaattcat gatctacacc
    16441 atggcggggt cacttggcct tttgcttgca gtgcagatgc tgggcgttct cttcgggacc
    16501 tatgacctgg tcacccttta cgaacgctgg aattcgctgg atgctggttc tgtcttactc
    16561 ggtatgccag tgcataccgt caaaaccatc gcgttctggg cgtttgttgt cgctttcgcc
    16621 atcaaagtcc ctgtttggcc cttccatacc tggctgccag atgcacatac tgaagcccca
    16681 actgcgggct caatgatttt ggcgggtgta ctgctcaagc tcggcgcgta tgggtttatt
    16741 cggctggtac tgccttttta tccgcttgaa gcgaagatct ttgcaggtgc gttggccttc
    16801 cttgctgtag cggccattgt ctttggtgca ttttcatcct atgcgcagac agacttcaaa
    16861 cgattggttg cgtattcctc agtcaatcac atgggattcg tcatgctcgg catcgctgcg
    16921 gcggggctgg cttctggcac gcaagatgcg catatcgcca tgagcggtgc ggtgctgcag
    16981 atgttcaatc atggcctttc ggcggcaggc atgttcttcc tcgtcggtgt tatttacgag
    17041 cgcacacata cccgcaacct caatgatttc ggcgggctct tcccgcttgt gcctgtgtat
    17101 ggtggcattt tgatcttcac cagcatggcc tcccttggcc tgcccggact taatggcttc
    17161 gtttctgagt tcatggtggt tcgaggcgct taccccgtgt tgaccacata cacggccatt
    17221 tccatgcttg gcctgctttt cacgggcgcc tacattctca agggtatcaa gagtgtttta
    17281 cacggcccct tgaacgaaca ctgggcgcat ggtgaacaca aactgactga aatcaacacc
    17341 cgcgaaatcc tggttatggt tccgttgatg gttctgatcc ttgtcattgg cgtctggccc
    17401 atgtggcttt tggacgtgat caacaaagcc gtcacggctt tgttctaaga ggtgatggat
    17461 gacgaatttt cccatcctaa gtgttattac cttccttccg atccttgcgg ctgccattat
    17521 tttgatgatg cctgcaaatc ggaagaatga agtccgcgca gtggcgcttg ccgctgccac
    17581 attagacctg ttgctctctg gctggctcta cctgcaatac ctgctgggag atatgacagg
    17641 ctatcagttc attgagcaat ataactggct tcctgcgctt ggtatcagcc tgaaatttgg
    17701 tgtggacggt atgagcaccc cgttggtgtt gctcacgggt attgtcatgt tcacgggtgt
    17761 tctcatttcc tggggtgacg aagacccgca tgtcaaagct ggtattcaag atagaccgcg
    17821 cgagttcttc gccttcctgt tcctgctggc aggcggtgtg ttcggcgtat tcgtttcgct
    17881 cgacctgttc atgttgttct tcttctatga aattgccgtg ttcccgatgt atttgctcat
    17941 tgccatttgg ggttgggtga agactcgcga atatgcagcc atgaaactga ccctgtacct
    18001 gttcatcggt tcagtggtcg cccttgtcgg cgcactggcc atgtattgga cccaatatca
    18061 gaatacaggc gtactctctt ttgacatgct tctgatggaa aatgcgaaat tttcgccgac
    18121 cttccagtat gtctggttcc tgcctgtctt cctcggtttt gcggttttgg gtggtatctg
    18181 gcctttccat aactggtcac cagatggtca cgttgcagcg ccgacggcgg tttccatgtt
    18241 ccatgcgggt gtgttgatga aactcggtgc ttttgccgct cttcgcgtgg gggtgatgct
    18301 tctgcctgaa ggtgcaaaag actgggcctg gctcctgatg ctttttgcag gcgttgcggt
    18361 ggtttatggc gcgttcatcg cctttgtcca gaccgatatg aagtatatga tcggcttctc
    18421 gtctgtttcg cacatgggtt tggtggtgtt gggtgtttcc acactcaatc atgacggact
    18481 tgtaggtgcg ggtgtgcaga tgttctcaca cggtgtgatg acggctcttt tctttgcggt
    18541 tacaggcatg atctatgacc gcgctcacac ccgtatgatc cccgaacttg gcggcattgc
    18601 caaagtgatg ccatttgcca cggtcggctt tatcatcggc ggtctggtct cgatgggcat
    18661 gcctggcttc tcaggttttg ttgcggaatt tcccatcttc atgggcgtgt ggggacgcca
    18721 atggctggtg gcagtgatcg ccagcatttc aattgtcatt accgcggcgt acatcatgat
    18781 caatattcgc aaggtgtttt tcagcgagat gcctgaaaac ctccacggtc acatcggtga
    18841 tgtcaccatg ctggataaaa tcgccattac aaccctttgt ctcttcatga tcgtcatcgg
    18901 tatgttccct gctgtcatgg ttcccatggt ggaaaaaggc gttgagaatg tgcttcgcct
    18961 gttgggaggt gcataatgtt tacatctaca actttcgctt cgatcctccc tgaaaccttg
    19021 atcctcatcc tgggaatcat cctgttgatc gttgagccgt tctggaaggc tgagaaccgc
    19081 cgcaacgttg gctggcttac cgctgtcggg ttgtttattg ccatgctggt gagtttactt
    19141 ttcggccagc ctggcgaacc cacctctgtt cttggcggca tgatccgctt tgactggctt
    19201 ggcttcttct tcaaaatgtt gttcatgttc gcgggcgcag ctacagccct gcttctgatg
    19261 gatcatgaaa aagcaggtct gcgcggcgag gcttacctca tgctgctcgc ttccatcatt
    19321 ggcatgaacc tgatggctgt ttcttccgat ctggtgatga tgtacctcgc cattgaaacg
    19381 gcctccattc cgctctatat tctttcgggt ttccttttgg gcgatgagcg ctctacagaa
    19441 gcgggcttca aatacctgct attcggcaca ctggcctcca cggtcatgct gtatggattt
    19501 agcctgatct tcgggttttc cggcacgacg aacatttaca aactctctga aatgctcgcc
    19561 gttggagaac ttccgctctt tgttaacttt ggcctgatcg gccttgtggt ggtgggtctt
    19621 ggcttcaaga ttgcggctgt gccccttcac ttctgggctc ccgatgtgta tcagggtgcg
    19681 ccgactcctg tggctggttt tctttccacg gcttccaagg ctgcgggttt tgcagtgatc
    19741 atccgcctgt tctttgtggc gtttcctgaa attgccaact attggacggt cattttggcg
    19801 gcccttgccg cggtgaccat gacggctggt aacctgctgg ctctctcaca aacgaatatc
    19861 aaacgcctgc ttgcgtattc ttcagtcggt catgcggggt atgcgttgat cggggtggtg
    19921 gccttcagcc agcttggcgc tgcaagcgtg gttttctacc ttgcggctta tattgcgacc
    19981 aatttgctgg cctttggtat tgtcatggct ttcagccgtg tcgctggctt ggaagacatc
    20041 accgattacg ctggtttgag ccgccgcaat cccttgatgg gtttgatgat gctggcggcc
    20101 ttcctttcgc tggcgggtat gcctccgttc ggtggtttcg tggcgaaggt cttcgtcttt
    20161 gccgcgggtg ttcaggccaa ttacacatgg ctggttgtgg tgggtattct caactcgatc
    20221 atcggtgtgt attactacct gaacgtgctg aagtatgtgt atctctatcg catgccgaat
    20281 gaagacgaag agaatcatcc cattccgttg actcgtccat acaccattgc gctggtggtt
    20341 ttggctctgg gcgtgatctt ggtgggtacg gtctttgctc cgtggtttag ctggtctgat
    20401 gcggcggctt tgaatctgtt ctaggaatta ggctttggac gatgggcagt agactgcggt
    20461 ttattgtcca tcgttttgat taggtgtaga aaaaaacata gaaagcagag tcaggtcaaa
    20521 ggtccagaca gatgcgctgt ttgggcggct ggaaaggcag gttggcggaa gtccgcctgc
    20581 ggtgttgaaa agggccgttc ggacgggaaa tcccaatttg gatttctgtt tgacagccct
    20641 ttgacctgtc agttataatc gtgcgacatg acacaatcaa agcagaccaa tcgtcaaaat
    20701 atggtgtata ccctgctcgt ggttatggtt gggcaggtgg gttgtttgac cattgctgtg
    20761 attttggcct cggtatttgg cgggttgtgg cttgataata cttttggaac gaaaccggtg
    20821 tttacactgg tgttgttgtt tgctggaatc cctctctcgg tcttcctgat gattttcgcg
    20881 gcgcggaaaa cgctggcgcg gctgaaggaa aaatacgaaa ccgaagagca aaaaactact
    20941 gcttaggagg cgggcttgga aacccaaaaa cgtaccccct ggcggcgctg gatcgtcctt
    21001 gtgctgatga tcgtgggctt tattctgggc ggaattttcg tccctgtaca gcctgagatc
    21061 acggttgccg ccgaaaaatt gatcgaagaa cccttgatcg agaactttct cggtcagccg
    21121 ttctatctgg tgaacacact cccaaccctt tttgttactg tgttcattat ccttttgccg
    21181 cttgcctggt ctgctggacg ctctttgaag aagagtcagg agaccgacct tgtgccgcgt
    21241 ggtgtgggca acacaatgga agccattttg gaaatgttgt acaacatgac cgaaagctcc
    21301 gctggcgtga agtgggccaa gaccatcttc ccgtggtttg ctaccattat gatctatgtt
    21361 ttggttgcga acctgctcaa actgcttcct ggctttgagt cgattggtgt gatccaccac
    21421 gctcatggcg aaggtcattt aacgcaagaa ttgggaaact tcttaggctt tcgatgggaa
    21481 ttgctttccc ctgataaagt tgaagaaggt ggctatatcc tggctccgtt cttccgcggt
    21541 atttccgtgg atctgaactt caccctctcg ctggcgttga tttccttctt tgccattcag
    21601 atcatcggcg tccgctcaca gggcattggt tatttcagca agttcttcaa cacgaagcgc
    21661 atgttcaagg tgccgttctt cggcgcgatg gatttcctcg taggtttgct ggaaatcatc
    21721 tctgaactcg ccaagatcct ctcgtttaca ttccgtcttt tcggtaacat gttcgcgggt
    21781 atcgtgttgg tggcagtggt ggcttcattg cttggcaaga tcagcattct gcctgcaatg
    21841 atcatgatgt ttgagttgtt cgtgggtctc attcaggcct ttgtgttcgg tatgttgacc
    21901 atggtattca tggcaatggc gacacagggt catggcgatg aagaacacgc tgaacactaa
    21961 gtcaaaaaat tcaattcata aattattagg atacccctaa ggaggcttgt aacatggaag
    22021 gaaatattct cgaagcaatg cgctttgttg gcgcaggtct ggcgatgatc ggcgctgcag
    22081 gcgctggtgt tggaatcggt ttgagcgtgc agggcgccct cacaggcatg gcccgcaacc
    22141 ccgataccta tggtaacttg ttgaccaaca tgattctcgg cgtggcgttc tccgaatcca
    22201 tcgctattta ttgtctggtc atcgcgttcc tcatgctctt caaagttgtg taattcaagg
    22261 agcaacgaaa catggaagct cttggtatta atctcggatt gctcattgtc cagttgatcg
    22321 cgttcgcaat tgtgttcctc acgctgaacg cctgggtgta ccaacccatg ctggacatga
    22381 tggaatcccg caaacaaaag atctcacagg gcctcgaaga tgcccgtgtt gctgctgaag
    22441 cccgcgcaaa tgctgaaaaa gaagccgcca aagtcatcgc tgaagctcag gctgaagcaa
    22501 gcaaggtggt tcgtgaagct actgaacgtg ctgccgccgc tggcaaggat gtcaaggctg
    22561 ctgctgaagc tgaagctgcc aaggcccgcg aaaccgcgat ggccgaagcc gaactcgaac
    22621 gcacccgcat tcttggtgac ctgcgcggac aggtttctgc cctcgccatt gccgctgcca
    22681 ataaactggt cggtgaagct ttggatgaga agaaacaaca cgccctcatt gatgaattct
    22741 tctctggcgt gaaggctggc aaggtggtgg ttgtggatgg tgaattcaag ggcgatgccg
    22801 ctgaagtcac cagcgcactt ccgctcaaca accaggaaca ggacgctgtt aagaagagtt
    22861 ttggcgccaa ggaagtatcc ttcaaggtca accctgccat tctcggcggt ttggtcgtga
    22921 agatcggcga taaggttctt gatggctccg tcgctggcaa actcgaaggt ctgcgccaga
    22981 ctttgaagta agcctgtata aatgaacacc gaaagggttt tggtaaaatt accaaaaccc
    23041 ttttttgttt aagcaatcgg agtgacatgc ttctaaaaaa actgttcgtt gattttccac
    23101 tttccatgcc tgaggttatc cccgatgtgg aaatttcagg cattgccatt gatagccggg
    23161 cggtaaaacc tggtcatctt tttgttgcca tgaagggtgg ttttgccgat gggcatgcct
    23221 acattcagaa agccatcgaa aatggcgctt cggcagttgt gggtgagagg gaattggacg
    23281 ggctttctgt cccatatctt cgactcgaaa acccgcgtca ggccctgacc tggctggctg
    23341 ctgcgtttta caattggcct gcccgcaagc tgacggtgat cggtgtgaca ggcacagatg
    23401 gcaaaacaac cacaagtaat ttgctccaca agattttgct cgcagcggga atcaaagcag
    23461 ggatgatctc cactgttaat gcagtgattg gggatgaagt gttggatacc gggtttcacg
    23521 tcaccacgcc cgatgcgcat gatgtgcagg cttacctcgc ccgtatggtg gaggctgggt
    23581 tgacgcatgt ggtgcttgaa accacctctc atggctggtc acagcatcgt gtggatgctt
    23641 gtgaattcga tatcggcgta gtcaccaata tcacgcacga acacatggat gaacacggta
    23701 gttatgaaaa ttaccgcgct gccaaggcgc gtttgttctc cagcctggag tggactcatc
    23761 ccaagcctgc tggaaatcct cgcctggcgg tcatcaaccg cgatgattcc aagtcatttg
    23821 gatttttgga tgagttcatc aaagttaaca aggtgaatta tgggttgggg aaggatgcag
    23881 atgtttgtgg gatggatatc gagtacaacc ccgctggcat ccgttttgtg gcaacctcca
    23941 aagattttcg tgtagccgcg tccagccgtt tggttggggc gtacaatgct tcaaactgtt
    24001 tagccgcttt atcggccgct gtgtatggat tgggtatcaa acctgaggtt gctgcacagg
    24061 gaatcgctgc tttggagggt atccccgggc gtatggaacg cattgatctg gggcagaact
    24121 tcactgccat tgtagatttt gctcataccc ccaatgcact caaagtgagc ttggaggcag
    24181 gcaggacgat gacacgggga agggttatat ctatttttgg ttccgcaggc ttgcgggata
    24241 aagagaaacg tcgcatgatg gcagaaacgt ccgcagaatt ggcagacctg accatcctga
    24301 cagctgaaga tccgcggacc gagtcgctgg atggcattct tgaagaaatg gcggcaggcg
    24361 ctataggaaa aggcgggcgt gaaggggaga ccttttggcg cgtacccgac cgcggcgaag
    24421 ccatcaaatt tgccttgcgc ttggctcgtg aaggggatat tgtcctttcg tgcggcaagg
    24481 ggcatgaaca gtccatgtgt tttggcaaaa ccgaatactt gtgggatgac cgtaccgcta
    24541 tgcgtgcggc gcttgcggac tttttaggtg tggatggtcc agcgatgccc tacctgcctt
    24601 cacgggatat gaatgagtcg gattggttga agggatgaac gctgcggctg tgagattatg
    24661 atccatacat tgcatcgtga gcagatcatt cccacttctg tggaagtgtt gtgggattat
    24721 ttttcggccc ctcagaattt gaacgacatg acgccacctg atatgaattt tcagatcgtg
    24781 atgggtggcg atgtgaaaat gtacgaaggg cagatcattg aatatcgcgt ggagttcatc
    24841 cgcggaatcc gctcgttgtg gctgactgaa attgcccatg tccgcgagaa ggaatatttt
    24901 gtcgatgagc agcgtgttgg gccttatcgg ttttggtatc acgaacacaa gtttgagcct
    24961 gtggcgggcg gcgtgaagat gaccgatcat gtcacttatg ctgcgccgtt cggatttttg
    25021 ggtgatttgg tcaatgctgt ttggattcgt cgaagattgg atgccatttt cgattttcgg
    25081 catcgaagga tcatcgaatt atttgggagt gtgaaatgac aaaaccttgg gcaactccag
    25141 tagtattaca gggaaaacat gtgcggctgg agccgttgag cgaagtgcac gttgatggcc
    25201 tggctgagat cggtgtggga caggattttt gggatttcat gttgtatggc cgcatggaaa
    25261 cgaaagaaga tatgcggaat tgggtcaagg atattatggg gcgtggtgaa aaagggaccg
    25321 atttgccgtt tacggtcatc catttggatt cagggcgggt ggctggtgca acccgatatt
    25381 tgaatatcat gcctaatgac cgtgggcttg aggtgggcgg tacctggtat ggacttgatt
    25441 ttcaacgtac gcctgtgaat accgagtgta aatatttatt gttatcccat gcgtttgaag
    25501 ttttgaaatg catccgcgta cagatcaaaa ccgattcacg aaatatccgt tcacagaagg
    25561 ccattgaaag gattggtgcg gtaaaagaag gagtgttgcg gaaccacatg attctcccca
    25621 gcggcatata tcgccattca gttttttatt ccattattga taccgaatgg gaacaggtaa
    25681 agaaaaggct ggaagcattc cttgaggggt gattttggcg attcgaagaa aaatccccgt
    25741 gtttatttca cggggatttt tgtattattg attaatgcct tttttgacgg ctttttttgt
    25801 gttatgcggt tgcctggccg acattcatga acgggccaag cagatacctg accacatctt
    25861 ccgctgtata actggttttg ccctctttga gaaactgccc gactgcaacg aggtcatcgc
    25921 ctgaggtaat gatgggttct tccacgtaac catccttgcg gacttgtgga aagcctgcag
    25981 tggctgtcag attgttgcac agacaggagc ggttgaccgt atcttcaagg ttgccgccct
    26041 ttttgacaaa aacatccaca ggttcggagg cgcagcgata gcctacagaa ccatcttcgc
    26101 gtttgtacaa gatgcgaagg aaaccgaggt cgcagatgcg gttgcgggcg ctgtaggcct
    26161 cagggtcaga cagtgtgcct tcgagttgtg ccactttgaa agggaatccc gtaggagaga
    26221 ccagcggact ggtgaatacc tgcgcctctt cagcgcggac cttttcgagc aaccgctgtt
    26281 tcaggtcggg gcgcatccct gattcttcac atagggaaaa ggcggtgcca acctgcacac
    26341 cagccgcgcc cgcttccaag gcagactgta cctgcttcgg atttccatag ccgccagcca
    26401 gccagaaagg caggccgagc gccttcattt tttccagatc cacggcgtct ttttcaccgt
    26461 agatcggctc gcctttttca ttgtagttga ccgtgccgcg aggtggggca ttgtgtccgc
    26521 cagcggtggg gccttcgatc acgaaaccat ccactgagcc ttcgctgcgt ttgataaggg
    26581 cctgtgccag taccaccgag gaaataatgg gcaggaactt gggccgctta agtttgccga
    26641 ccagcttggc gatgccaggg aagatggcct cagggtcaaa gtgaatacgg tggtcatcat
    26701 ccttgcctgc gccgtgaaca tcaaggcgat acgaaacagg ctcatggttt gaaagtttgt
    26761 cgaggatgcc agcgatctgg gtgggaatcc ctgctcccat caacaccacg tctacacctg
    26821 cgagcatggc gccgtagagc gaggccaggg ttggcatctg cactttttcg agcaggttaa
    26881 tgccaaccag gccgttgtga ccttctttgg caaggaaaac ttccacgtaa ttggcgatcg
    26941 ccgtcacctg atcgaggaat ttgggcggtt tgatggtgta ggccgcaggg gttttgtatg
    27001 gggtttgcgg tgattttcca cctggggtgt aataccgatc aatgatctgt tgaacgggct
    27061 ctggtaaaga gaagttggac agggcgcggc gtacatttcc gctgatatcg ccatccatga
    27121 ggcggctggc taaaactcgg ctgatgcctg tgccagagac cacccctaat tgcccgaggt
    27181 ttgaaacagc tttggcaagc ctccaatcag aaatggcaac acccataccg ccttggatta
    27241 attttggtag attcattcat tgactccagt gattagtatt aaatctggtg cagggttaca
    27301 tgttaataac ccctttgggc ggttatggtt ctgcgtttat gaaaacattc tcagccttca
    27361 gcggcatcgt cgtagggatg ttttgcgaga atggcctcga attctttttg ttttcgctca
    27421 gcccaggcag gcttgaccat cattaaatat tcaatattct tggcgaacag caggcggcgg
    27481 aaactctgtc ctttgaccct gtgtttgaat tcataggcac atgccttgct ccaggcaatt
    27541 tcatctacaa gccagtagag caattcgttt ttgtcgctgg tttgcttttc gctgaggatg
    27601 agtccgcgct cggtggcgat gtaatgatag gagttgtctc tgatctcgag gtgcgaagtc
    27661 ccatcatcgc ctctgatggt ttggattttg acatgggcat aggctgggtc catgcggcgg
    27721 caatactcgc ggagctcggc ctggattttt tcgaaggatg gctcgaacat ggtcaactct
    27781 ttcggggtgg agatgtttca ggaagccgaa tcaggtttcg gttgcgggcg aagcgctgcc
    27841 tgggcgaggg ttcttttatt aaggatgagc agtcccgcag tgctggtcat gccgatgagc
    27901 agacctccga tccagatggc acgaggcgag atgctgtcat tgatgaatcc accgataagc
    27961 ggcgagagcg tgcgggcaag gccccaagcc agccaataca ccgacatgta tcttccgcgc
    28021 aggtcggcgg gggcggtgtc agccacatat ttgcttgcag tggggacaag tgtaagttca
    28081 ccaaaggtga gcatcaccat gcttaaccag aatccccaaa agcccgacat gagcgccacg
    28141 cttcccacac tgatggcgta gataaacatg cctgccgcgg ccacgggcag ggttttgtag
    28201 tgcctcgtga ttcgcgtgac cgcgtattga atgaaaacgc acatgaccgc attggtggtg
    28261 gggatccagc catacagggc ttcagacaaa ctgaaatttg tctttgcgta cacgggcatg
    28321 aggatccaga ccatggtggg ggcaatgagg ccgatgccag tgaggacgac gaaggccata
    28381 aaatcgccgt ctctaaaaac gcggacgaat ccttccataa cggagggttg gtcggctaaa
    28441 gaatggccat ttgttgcgcc gacagttttg tggagtgttt catgtgcaaa gagaaacatc
    28501 agcaggctat gggcgaaaaa gcccacagcg gcgccataaa aagcgagatc gtatgaggaa
    28561 gctgccagaa atccgccgat ggctgggcca agggcaaacg ctgcattgtg agcgatgcga
    28621 ttgatcgcga atgcgtcggt gcgttgttcg gtagggatga tatctgccat catggcatcg
    28681 gcgcccactt gatagagcgg gttgcataag ccgatgaaga tcatcaaagc gatgaactgt
    28741 ggataggttt cagcctgcat aagaaacagg taggcgatgc cgtttacgac caggctgaac
    28801 accatcactg cttttcggcc gattttgtct gtaagagtgc cagcgaggaa ggaggcgagc
    28861 aggcctgtcc ctgcgttgat ggaaatgagg gtggctacag tgctgagggg taggttgagt
    28921 tttccgcctg catagataag cagaaagggc cagatcatgc ttgcgccagc cgtgctgatg
    28981 acgatgcctg ccaccatcaa ccagaactgg cgtggatatt gattgaagag gattttgagt
    29041 ttggaagcca tgggaagtcg agtctaacat atcctcactg tcgggtgagg aggtggaatg
    29101 gggcctgaga tcaaaggtga atggcggtgt ttgttggcgc gatttttgga tccaggtgtt
    29161 tttatttatt tggagatcgg tgtagcaagg tagtcttcca tctggcgggt ttcgtagacc
    29221 tcacgccaga ctggttgaat ggctttatgg atggcttgat aatttagact gtcttttcgg
    29281 atggctaggc caaaatcgaa ggcgccagga tagaactcgt actgctccca aaatgtttga
    29341 gaatcaatga tctctgcggt gatcttggtg taatcgaaat gaggaaacca gatgaggtca
    29401 ggtttgcggc gcaggaattc gtctacggag aaaccgttat gggcgaagta ggaatcgtgc
    29461 agtccaataa tgtcaacaat gtagaggtgc ggtgattccg cgcctgtaaa tccatattcc
    29521 gattgggcaa aaatgattcc ttcaggaagt gattttgaga atttggctac tgcgagaacc
    29581 atgggccatg ttccgcgttc aggaagttct tttgcggcgg gcgtggtgta ttctgtgtga
    29641 ggagagtaca actttgtggc agcgatctgt gagtttttgt atgcgtcact ggtgatcgtt
    29701 tttagagtgg agttggaaaa gagtgcgaca atcagaagga gaatcaggac ccgcatgagt
    29761 cttttgccgt gactttctcc ttccgtatct cctgaagaaa gcgagcggtc aagcatgagg
    29821 atgctcagaa cgatcaggta cgggagggca gggaaataat atctggcttc aaaacccatg
    29881 atctgaacaa ctgtgaaata ataggtgaag gtcagggcaa tggggagcaa aaatacggcc
    29941 agtaatttca gagtctgttt tgtggatgac aggacgatca aaacaagaaa aggcagggca
    30001 agggttccga aggcgaatag atattcgatc gggttccagg tgtgcgtgcc gatataacct
    30061 tcataaaagc cgctggactt ggcataaaac ggcaggggta gagggtcaga gaagacgatg
    30121 aacttcacca acgtatcgag tgcaagaaga acgcccagcc aacctaaaaa atggacaagt
    30181 cgttttctcc tgccttcagg agcaagaaat aggatcgcca atactggaaa gaaaaatgta
    30241 taaagcaggt tgtcggggcg tgccagaaag gcaagatacc ctgcaatgac cgtcagggtt
    30301 gaaaaaagcc tgtaccgatt ttcgttgtaa acccagccaa gcgtggagaa gataagcagg
    30361 gcgttgcaca aaaaagaaag cgtggtgtcc attccgctct tcatgtggaa caggtataca
    30421 ggtgctgtaa ccagatagcc aacaagcagt acggtcaacc aaagaaaaga gcttttcagg
    30481 agttttgact cggcgtacct gctgactgta taggcggtca acacaatggc gggcagccca
    30541 agaccagcgg aaagggcaac aagcaatttg cctgatccca atccttggaa aatgattcgc
    30601 gcaataatga cgagacccag atacaggata cttgtggctc cataggtttg aacgccatct
    30661 gggttccagg caaagccgta tccgtggatg aaattatccg cgtaacgaat gaacatgtat
    30721 gcatcatcaa atgtggttgg ccctgctgtt ctgaaaacat cgatgtaata tagcagcaaa
    30781 gcagcgagta tgattaccag agcggtaata cgcagtctgg ggtttgaaaa gaaggagggt
    30841 ttatccttga ggtcgttcac agcattgact ccaattgctt ttacagaaat agcacatgta
    30901 aaattatagc actgtcataa aaaaggacgg gtgaatgctt tcacccgtcc ttttgattcc
    30961 tgagaaaatc ttttatctac gtccgccgcg gtccccgcca cctcgacgat ccccaccgcg
    31021 atcgccgcca cggcctcccc tgtctccgcc gcgtccgcca cggtcaccac cgcgaccccc
    31081 acctccgccg ccgcgcccac ccttgtcttt ttcacgggct tcttcggctg accagccttc
    31141 gagtacagcc tgacgcgaaa ggcggatctt gccctgcggg tcgatatctg tgaccattac
    31201 ggtcaattcg tcgccgagca cacaaacatc ctcgactgtg ttgacgcgtt cactagccag
    31261 ttgcgaaata tgaaccagac catccacacc gggcagcagg ttgacgaacg caccaaatgc
    31321 ctcaatgcgt acgaccttgc ctgtgtaaat attgccaaca attacagact cgccaagacc
    31381 ttccacgcgt tcctgggcga tctttgcgcc cacgctgtcg gtcgaagcaa tgtaaactgt
    31441 gccatcttct tcgatgtcga tctttgtgcc agtctcttcc tgcagggcgc ggatattctt
    31501 gccgcccggg ccgatgagcg caccgatctt gtccacgggg attttcacag tgatgatgcg
    31561 aggagcatgc ggcttcaatt ctgcccgtgg ggcatccagc actgccaaca tcttttccat
    31621 gattgacatg cgcgctgtgt tggcttgcgc caaagcttcc ttcatcattt gcgcgctcaa
    31681 gccgctgatc ttgatatcca tttgcagagc ggtgatgccc tgtgcagtgc ctgccacttt
    31741 gaagtccatg tcgccgaggt gatcttcggt tccctgaata tcagtcaata ttttgtagcg
    31801 tccagtttcg tctgtgatga ggcccatggc tacaccggaa acaggcgcct tgatcggcac
    31861 acctgcatcc atcaaggcaa gggttgagcc gcagaccgaa cccatcgaag tggagccgtt
    31921 ggaagacaag gcctcagaca ccacacggat cgcatagggg aaggtctctt cggatgggat
    31981 gactggctca agtgcgcgtt cagccaacgc tccgtgacca acctcgcgcc tgctctggcc
    32041 gcgaaggggt ttcacttccc cggtggagaa tggcgggaag gaatagtggt gcatgtatcg
    32101 cttgctcttt tcaggcgaca ggttgtcgag ttcctgtgct tcacccaggg tgccaagtgt
    32161 ggcaaaggaa agtacctggg tttcaccacg ggtaaacagg ccggtgccat gcgcacgcgg
    32221 gaagagaccc acttcacacc agatcgggcg gatctcggtg ggggtgcggc catcggggcg
    32281 cttgcccatg ctcaaaattc gctcgcggac gatcttatgt tcggcttcac tgaacgccga
    32341 tttgacatct ttttcagagg gggcattctc atcgccagcc acgcagaatt cggcaatgat
    32401 cgcggtttgt aattcatcca tgccgccgta gaactcagct ttgctcaggg gcttatcgag
    32461 caattcgttc atgcgggcat tgaccgcatc taagaccttc ttttgcaggt cttcgttggc
    32521 tgtgacgatc gtagcctcac gcttgggttt accgatctcg gcctgcatct gaagctggag
    32581 gtcaatgatc ggctgaatgg atttgtgtcc cagttccagc gcttcaacca tcacatcctc
    32641 gggaatttcg tttgcaccgc attccaccat gaggatggca tctttggtgc ctgcaattct
    32701 cagatcgagg tctgaggcgt tcatttcatc gaaggtgggg ttgagcacga actcgccgtt
    32761 gatgcgtccc acgcgaacag cgcccaccgg tccgccccac ggaatgtctg agatcataat
    32821 ggcggcggag gcggcattga tggcaagaat atccaacggg ttgacaccgt cggatgagaa
    32881 ggtgtacatg atgacctgca cttcgttgcg cattccatgg gcaaagagcg ggcgcagggg
    32941 gcgatctgtg agtcggccag tgaggatggc gtctgttgca gcgcggccct cgcgacggaa
    33001 gaaggaaccg gggatttttc caccggcata catgcgttct tcaaaatcca cagaaagggg
    33061 gaagaaatca atgccgtcac gtacaccacc catggtggct gcggcaaaaa cgatcgagtc
    33121 ttccactccg aaagtgaccg caccacccgc ctgagcggcc agtttgccgg tctcaaaggt
    33181 gaaggtgcgc gtgcctacaa cggctgaata gcgttttgat tcgggtttca ttattttatt
    33241 gtctcctgtt gcgcgagata cgatctcgct gtactaattt tccgccaagg gtcagggact
    33301 gaagagtggg aacaacctcg gttgttcgcg ctattcaatt cctgacggcg gtgcctattt
    33361 acaatatagg gacacggcgt cgccgtgtcc ctacgggtta aaacttttta cttgcggcgg
    33421 agctgtaagc ggtcggttac actgaggtag ctctggtaac tgcttgaacg caagtacgtg
    33481 agcaagcggc ggcgttgacc taccaacttc agcaaaccgc ggcggcagga ttgatcctgc
    33541 ttgttgcttc gcaagtgctc agtgagctga ttgatgcgat tagtcaggat ggcgatttgc
    33601 acttcaggcg aaccggtatc cttgtcgtgg cgatgaaaat cttcgatcgc ctttgtcttg
    33661 acttcttttg caagcggcat gacgtatgct tcctttcttt ttagattacg caggtctcca
    33721 tgcccacagc ttgagaccat agggcaggac cagtcacgaa gcgggattat accagaaaac
    33781 cactcccttg gatgaaaaaa acgcctcttt tcgacctgaa acctgcttgc ccttctggaa
    33841 gcagggtcag cgccgaaggt gagtctgttt tacacctcga tctgtattgc actcccgctt
    33901 tcatagcggc gcagacccat gcgaaaggtg aaaactgcca atgtaaggaa cccgatggcc
    33961 cctagaagca gttccccgag ggtttgccag gtaaaggcac ggataaactc tgcagggatg
    34021 gctcccatca gcgcagcagg gataagggta aaaaggataa gtttcgcgta gctatcgaaa
    34081 agggtgatcg ggtagatgcc aaaggttagc atggcattga gtacgaggtt actgaaattg
    34141 gtcatcatgc cgaaccagaa cgccagactc tgtgtcagga tgagaaatgc cgcgaaaaca
    34201 gtagcagcta acagcatggc aagaacgtag cggagcaatc catcccaggt gaaatatcca
    34261 gaaagtccat aactgacgaa gccgtagaga aaatctccca ttccgctggc gatggttcgg
    34321 ctggctatgg tatgcaacag aaccgggcgg ggcagggaga ggtagtaatc aaggcgaccc
    34381 ttggcgataa tatcgctgag gttgaaggcg tttccgaaaa aaagactcac gaggccaaag
    34441 gcactggcca gcacaccaaa ggtgacgtac atgtcgttga cgccccaacc gcgcacatct
    34501 ttgaaacgat cgaaaaagat gatccagatt gcgaagtaaa tgaaattatt gaccatcatg
    34561 ccaatggctt gcgtcaggaa ggccgcacgg tattccatgg cagattgcag gttggttttc
    34621 caaatggcga aaaggaattt cagttctttc atatgacgat atcctgctag cttgtacgat
    34681 gagaccagtt ttttcagccg ccattcactg tcaattgtgt caacccgcgg cgatagacaa
    34741 tggttagcgc aacgaccaac gcaacgatcc atgtgacttg cagggtcatg acgctgacaa
    34801 aaagatcata ggttggggtg acaaaaagcc gcgcaggccc ataaatggcg ctggagaagg
    34861 gcagcaattt tgcgaggttt tgcagccact gagggtaaaa atccagcgga atcaacatgc
    34921 cgccgaaaat gaacgccagc ttttggtaga tccacgtaaa tgcggaaaca tcctccacga
    34981 caaaagcgga gagtccgatc atggcattga tggcaaaatg caaaacccag gccccgagaa
    35041 tggcaggcag tgcgatcaga aatccttctg ggtgattggg tgtgcccacc agccaccaaa
    35101 tggtcaaccc gccaaatacg gcattgagca tggcacggaa gatggtttcg cccatggcag
    35161 tgctgaattg gtagagcaaa aaatcgtatg gcttgttgag aatgtaggca atggaaccat
    35221 ctttgacgtt ttcagacatg gtgcgggcaa tgggtggacg tcccagttcg atggtctctg
    35281 ccagcatgag ataccagagc gtgtcgcgca gggtcaatcc attgatcatg ccatcaacag
    35341 ccccagccgc gctgaaggtg accttccata gttgatagaa gatccacata aacgggatga
    35401 tcatcaaact gcgcccaacc aactcgccag gataggcgag cgagttcaaa agcgtgatct
    35461 gaaagatgga ccagtatttg gaaatggatt tcatggaaaa gacaggaaat agtttgtttt
    35521 gatcacagcc gaagaacact atggaaaaaa cactcttcga ctgtgcccaa gaatgtttct
    35581 aaaatggccg catgcttgcc gccgcttcga gggcgagtgc tgataactcc tcctggcggg
    35641 gcagttcttt catctgaagg atacgcaggt gaatggctcg cagcaggttg cgggcgttga
    35701 gataggctcg ggattgttcc tcatgatccc atgcaccttt ccagtcagtt gtgaggtttt
    35761 cgaaccaggc cttgacctgc tcaagtgata gttcttcgcg ggcaagggcc gtgacaatgg
    35821 cacgggcaag gcggtcatct tcgccatgga tgtaaatcca atttttggcg gcgcgcatct
    35881 tttcagagac ggcagacagg atgtgttcca attctgttgc acccaggtgc gggctgcggg
    35941 caaataccat cagcatatct gcggtatggg ctaatgcatg tgcccaacct ttgacgggaa
    36001 cgtatccacg cgggtcacgt tctgcggcga gataggcaag gacacgagtc agggtttctt
    36061 ttacttcatg cgactccaga tatggttgtt ggttgtcgcg gtggatcact tctgccagat
    36121 aaagcacgga aaatgcgcgc ccaaatacac tgtcgtcatc ttgctgaccc agaccaactt
    36181 gcaggttttc gagcagacgt ttcaggtaac cgcgtaattg ctcagcctgg tagggctcgg
    36241 tctcgatcca gttggcaaag atttcgtagc caatgccatc gcgtagatct gggtcggtgc
    36301 tggggatgta ggagaataat tcatcggtca atgctgtaag gtcgtgaccc tgagggaagg
    36361 catatttatt ttccttgatc gtgtgccaga aatttttgtc cataattgat tgaaacgcta
    36421 ggcgttccct gcctcctgat aaatttttga aatgacctct tccagcggcg ggtcggagat
    36481 ggtgatatcc acgatgtcgc ctgcgctgga gaggtgagag atgaccgtat ccacaggtgt
    36541 ttgggttgta tcgaatttca acttgacacc ataatgaccc actttcaagg tttccacacc
    36601 ctgaatggca aagtctttgg agacctccaa ggcatagcgg acctcgacca attttgtggt
    36661 gaggaatttc cgcttcaggt ttgagacctt gtcttcgtac acgatctgtc cgtgattgac
    36721 gatgatgacg cgtttgcaca gggcttcgag atcgcctgca tcgtgtgagg tgagcaaaac
    36781 gcccacacct tcctcctgat tcatgcgttt gatggcatcg cggatgtgtt gtttggcgac
    36841 cacatcaagg ccgatggacg gctcatcgag caggagcagt ttcgggcgat gtagaagcga
    36901 ggcagccact tcgcaacgca tccgctggcc gagtgagagt ttccgcacgg ggacatccag
    36961 caaatccttg atctcaaagg cttcggtgag gaagtcgatg cgttttttga tttcacgatc
    37021 atccagttcg taaatttttc cgaatagcat gaaggtgtcg atggcgggca ggtgatacca
    37081 aagctggggc ctttgcccaa aaaccgtgcc aatgtgatag gccagttttt gacgatcgac
    37141 ccagggtgtg aaaccgagta cctttgcctc accgccgctc gggtgcagaa tccccgtcag
    37201 cattttgatg gtggtggatt tgccagcgcc gttcggacca atgaaaccca acagttcgcc
    37261 tgtgcccatt tgaaaagaca ggttgtgtac cgcttcaacc agactgtact ctggcttgaa
    37321 aagcgaacga atcgaaccgc tgagccctgc ggccttgcgt ttggtttgaa aggttttttg
    37381 aaggttgctt acatcgattg agagcatgtt attataattg gcagtagaat cggattgata
    37441 attatagcgt gaaagaggta ttttaatgcg aaggctatcc tgggtgattg tgttggcgtt
    37501 tttgttgcag gcatgcgcgg gttttagttt aaacccgcct acttccacgc caacgattac
    37561 ttcaacacca accattacgt atacaccaac catcactccg accattacca ttacacccac
    37621 ccgcacagct ttgccaacca ttgttcgaat cccaacctgg gatccgaatc agccgacacc
    37681 cacttttatg gcgattccca ttttcatcgg cagtgtcact gctacgccga tcgtttcgcc
    37741 gacaccgacc cgcccaggga tcggatttgt ttctgtggac gtttctgagc agaagatctt
    37801 ttggggaagc tgcaagcata acaaggcgaa gatcattgcg aaggtacaaa accccgacga
    37861 agttttcagc gtggttattt ttgtgcgtgt aaaagcattg ttgaaggaag attatacgcc
    37921 ctggaccact ggcgatgtca tgaccaaaca tggtgatgga acattctcct acacccttgt
    37981 gggcagcgag atcgaaggcc ataaccatta cagggattcg tgggtgtact ttcaactggt
    38041 tgcgaccaat gaagaaggtg aaattgttgg ccgaacgatg atttacacca attcactttc
    38101 gctctcgccc tgcaaataaa caaaggatgc gatcatgaaa aagttttatg ccctttctct
    38161 ttgcaccatt atgctgttat cttgttcgtt gaatttgttg gcgcccagta taagttccac
    38221 gcctgtgcct acgctgacaa acacacccac cgacctgcct tccgccacac ccacagtgcc
    38281 cacacctact ttcaccccta cacctccatt ggttggcttg cggacccata cgctaacccc
    38341 aaccatcacg gcaacatcca cacagccaac tgaaacatcc atctttttgg ccacacttga
    38401 tacgccaaca cccagcgtgg aaatgaaggg ttttgtttcc gtcaatgttt caaccaaaga
    38461 gtttttcaaa gatgcagaat gtcagccgac ttcagtgaag ttcgttgccc aggtggctga
    38521 ttcggcgggc accgcttttg tggttctttt tgtacgtttt aagagcaagc aaacaggaac
    38581 gactagcgaa tggaccagca tcggcatgtc cacgatcggc gcgggcacat atacctacga
    38641 attgaaacca gaagagatga aaggtgtgga ttctttcaag aacccatggg tgcaatatca
    38701 atttgtggca accaatgccg atgcgaacga aatcggacga acgggaattt tcagcgaacg
    38761 cttatcgcta ttggaatgtg aaccgacacc cagcccaagc ccatcacccg aaccgacggt
    38821 gttgcggcca tgatggaatt ccccgatgac ctgatccgct ttttgacgaa ggccacccgc
    38881 gtctctgtct taacgggagc tggtgcctca caagaaagcg gactccgcac ttttagagac
    38941 gctcaatctg gactttgggc acagtacaag cctcaggacc tggcctcacc tgaagccttt
    39001 gcccgcgacc ccaaactggt ttgggattgg tacgcatggc ggcgtgaagc cattaaaggc
    39061 gtacgtccaa atgcggggca ttatgccttg gtggatatgg agaaaaaggt tgaggtgttt
    39121 acgctcatca cacaaaatgt ggatgggctg caccgcatgg cgggcagttc gaatgtgctc
    39181 gaactgcatg gaaatatctt aaaagtacgc tgttctgaat gcagaacatt tacggaagtt
    39241 tggggtgacg attcggaatc tgtgccgctc tgtgataagt gcggcggaat gctgcgcccc
    39301 gatgtcgttt ggtttggaga ggcgcttcca cgcgaagaac ttgaggccgc ggtcaatgct
    39361 gcgcgttcgt gtcaggtctt cttttccatt ggaacatcgg gggtggttca gcctgcggca
    39421 gccttggccc atgcggcgcg caataaaggg gccgtggtgg tggaggtgaa cgcagagccg
    39481 actccgctga ccccgaaaac cgattttgca tttcacggta aatccgggga gattctgcct
    39541 gagttggtta gggcagtttg ggggtagttt gagtcgggct attcacggcc aggcgacttc
    39601 caggtaaatg gtcacgttgt cgtccacgcc tatgttctcc gatttttgaa cactcattcg
    39661 aatggggaca aggtagcggc catcttttgg aaatagggag gttttccatt ccgtgcttcc
    39721 gatccgcacc tgaactggga tgacacccca gccatatgtg acgctggagg agatggcttt
    39781 caaatcacgg ctgggttttt ctgggacagc cacaaagaga aagggggcag ggccacgcca
    39841 gtgaaaaatt tttcctttga atttgatggt cat
//
LOCUS       DAOQLC010000022        39820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_283_length_39820_cov_21.164567, whole genome shotgun sequence.
ACCESSION   DAOQLC010000022 DAOQLC010000000
VERSION     DAOQLC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39820
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_283_length_39820_cov_21.164567"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2593)
                     /gene="mutS"
                     /locus_tag="PL000_05845"
     CDS             complement(<1..2593)
                     /gene="mutS"
                     /locus_tag="PL000_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC88451.1"
                     /translation="MPSNDDLTPVRQQYLEIKREHPDRIVFFRLGDFYETFDEDAEIT
                     ARELDIVLTSRPVGGGVRVPLAGIPYHAVENYLARLIEKGYHVAICEQVGEQPVKGIF
                     PRKVVRVVTPGTVTEPGLLPGDANNYLASVILDTTTAVNSNPITASVSYVDITTGEFA
                     VTELPLEALRAELTRLHPAEIIHPDSQVLPDGITGHITALPAWKFEPGKCSEVLISHF
                     KASTLAGFGLKDNSLSARAAGAIVQYLKETQPDALNLLTSLRAYSLNEFMTLDASTRR
                     NLELDETLRGDPANPTRGMRKGSLLGTLDFSVTPMGKRLMHQWVSQPLLHVAKITQRQ
                     NGVQYFVENGMVRAELRATLKPLADLERLVNRVLAGQAQPRDLVAMRTTLTELPKIKE
                     VISGQKAVVSDQWSVCTQELSLLQNAIDDDPPATLQNTGVIRPGYSSELDGVIDASKH
                     ARDWIANLEGVEREKTGIKTLKVGYNKVFGYYIEISRGAADKAPESYIRKQTLVNAER
                     FITPEMKEYETLVLNAEERIKEIETRLFREVCAELVKAANQILATARAIAELDVLSAL
                     GEAAALGGYTRPEVHEGSGLEIHEGRHPVVEQLLKSDRYIPNDVIFEKGEVVRVITGP
                     NMSGKSTYLRQTALIVLMAQMGSFVPAASAKIGLVDRIFTRIGAQDEIHAGQSTFMVE
                     MVEAANILHHATPRSLLILDEIGRGTSTYDGLSIAWGIIEYIHNHPQLRAKTLFATHY
                     HELTQLADLLPGIRNYNVAVSEADNKVVFLHKIIAGGADRSYGIHVGQLAGLPAPVIQ
                     RATEIMAELEKTSGRAVKIDPNAAQQVALFPETNPLLDELKELDVNSLSPIEALNKLF
                     EWRMRFTK"
     gene            2704..3231
                     /locus_tag="PL000_05850"
     CDS             2704..3231
                     /locus_tag="PL000_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88452.1"
                     /translation="MFNHRLPKLNDVLSVYAVIATMLFAWSALLFFWYLPSWMHFMLL
                     GEIAATFSYVVIASFLEGLSFLLFLLVLCFFLPPEYLRDEFAARGTALTLPIIGMIMI
                     YFRITNENIARIIFSVTGLVISLVVILLFYFSANNPRIKSAFAALAERFTVFLYILIP
                     IFVLSLLGIALRNIF"
     gene            3241..4773
                     /locus_tag="PL000_05855"
     CDS             3241..4773
                     /locus_tag="PL000_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC88453.1"
                     /translation="MKSLSRRDFLKTAAAFSAGSLLSMAPALQNIKAQSNKPNIIVMV
                     FDAMSARNLSVYGYPRSTTPGLERIAERATVYHSHYSAGNFTTAGTASMLTGLYPWTH
                     RGINLSGMVNKNHVDHNIFNLIGDEYHRTAFTQNYLADVLLGQFQSGLDRHIPAPTFY
                     DQLDHPLIGQYFPRDPIPAYYGLDDYLFSGGIDRVGSIPSAPIFGFLNKLARRNQDFN
                     KSLPDYPYGIPNNTFYSYKHEVVYAGVLDEIKNLASEQNPFLAYFHLFSPHEPYRPRK
                     QFTDIFPELEVPFKAPTKFSTLDIDNDHILWRRKTYDEFVADVDSEIEKLVNALDESG
                     ILENTYLILTADHGELFERGEYGHVTRLLYDAVVHIPLIVIAPGQKSRVDIHHPTSNI
                     DLLPTLLHIAGKTAATDLEGRILPGLGGIEESRSLFSMEAKECSAFGNLKDGATVSLV
                     QGEHKLIYYTGYPKRPDTFELYNLKDDPDEMKDLFTVDTTTAAKMKEEMLDTFESNRK
                     LK"
     gene            4845..5006
                     /locus_tag="PL000_05860"
     CDS             4845..5006
                     /locus_tag="PL000_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88454.1"
                     /translation="MYIDPNTGGILFQALAAVFVAGSGVLFFFSRNIREGIAKLRRRF
                     RKEDEEQNQ"
     gene            5016..6839
                     /locus_tag="PL000_05865"
     CDS             5016..6839
                     /locus_tag="PL000_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC88455.1"
                     /translation="MKTIILGFDSFDPAVFEDMAGQNKLPNFSKFMDAGGYSRLEVCS
                     PPQTEVSWTSIATGADPGGHGIFDFVHRDPATYAPYVSILPMKKSAMGEQFVPPYIAK
                     TLFEEAASMGYPATALWWPAMFPARPELPVNTLPGLGTPDIRGQLGVGTLFTTEDEKK
                     SKTAVTRFESTGKGRYSALLNGPQVQGKNGPQPATLPIQLEALDQTSARVTIGDQQIQ
                     LRVGEWSEIVEVKFKAGLFLSIHAITRMILTSLNGVVRLYTLPLQIHPLHALSHYSSS
                     KSFAKDLWQKVGPYLTLGWPQDTTGLEDGCITDEQFIALCDLIFERRKQIFFHLLGNF
                     KEGVLGSIFDDLDRVQHMFFHNRLDVAQAWYQKLDAFVGEVSQRVEKWGGKYNYLIMS
                     DHGFTTYQQKIHLNRWLAENGYLATSNGSAEGDLSNMDWQKTKAYAVGLNSIYLNVAG
                     REGKGVVGANEIESLLAEIQQKLMGWNGPDGRPVMQRVRFKHEVYNGAYTRLGPDLVI
                     GYAPGYRASSETGLGKVPQSLVEANHDHWGADHCVDSDVVPGVIFANRDLQNFGGVSF
                     RDIPFLAIGKHLDQSHIKPPSQVSGGSQKDLEDRLKGLGYL"
     gene            6994..7602
                     /locus_tag="PL000_05870"
     CDS             6994..7602
                     /locus_tag="PL000_05870"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC88456.1"
                     /translation="MKNLFSRQTPKKTNEKIVVVSGLPRSGTSMMMKMLAEGGLPTVV
                     DSLRQADEDNPNGYFEIEQVKALKDGEKNWIYDARGKVVKVISYLLEYLPNDLEFNVI
                     FMDREIHEVLASQKKMLEHRNEVSAISDEDMEKQYREHLKAVRYWLPRQPNMRVLYVK
                     YDQMVKSPQTLVPSIIEFLERPLNAEAMAAVPSPTLYRNRKP"
     gene            7653..8399
                     /gene="gpmA"
                     /locus_tag="PL000_05875"
     CDS             7653..8399
                     /gene="gpmA"
                     /locus_tag="PL000_05875"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNC88457.1"
                     /translation="MYKLVLVRHGQSTWNLENRFTGWTDVDLTDLGRSEAREAGKLLR
                     EGGYDFDIAYTSVLKRAIKTLGLVQEEMDREWLPVVRAWQLNERHYGSLQGLNKSEMA
                     EKFGEEQVKIWRRSYDVPPPALELTDERHPKFDRRYASLTPEQLPATESLKITLDRVL
                     PFWHSTLAPEIKSGKRVLIVAHGNSIRAMVKYLDNISEAEITELNIPTGLPLVYELDE
                     ALKPIKHYYLGDAEEAAKKAAAVANQGKAK"
     gene            8490..9047
                     /locus_tag="PL000_05880"
     CDS             8490..9047
                     /locus_tag="PL000_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88458.1"
                     /translation="MKLLKLISERMPDSKNILAVYAFIVMLVYSWTLFTSFYKLPSWL
                     FYLSIGQILSVYAYAFSFDLLESLIALTVILLLDYTVFLFLKNKEEFQSRSMILAGIL
                     LGTSMTRLALFQNYEDITAFLSGELIWWAIAIPLGLVLAAFAPRIKLVRTIFEGFAER
                     ATVFVYIYIPLSLISLVVVLIRNIN"
     gene            9056..10597
                     /locus_tag="PL000_05885"
     CDS             9056..10597
                     /locus_tag="PL000_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC88459.1"
                     /translation="MTNFSRRDFLKLLGAASVGALASERVLSLKAADNSKPNIIIILW
                     DAFSARHLSLYGYPRQTTPNIDAFAEYSTVYHNHYSGGNFTTCGTASMLTGMIPWKHR
                     AINYDGLVRAEYIHNNPYTLLGSDYKRLMFAQNPWPDRLVGQYYEDVDRFLPPTAFSL
                     LEDSPVFFENDRAIASVAMSSFLMPIQGGNAPVGSSILGYMNKSRVLNFADGQKSPRY
                     PKGIPEAMNMGYMIPYLNEDVYEGLFSEMTQLEKNGDPFFSYFHLYSPHYPYRPRNDY
                     LKMFRDDGFAPVSKPIHPLSVGLQQDYLNGQRDMYDRQIAQIDEEFGRLIPRLEESGV
                     LENSYIIFTADHGELFERGFAGHGFQFMYESVLHIPLIIHAPGQTKRQDIHALTSNTD
                     ILPTILSIAGKETTPDMDGRILPGLGGEVDEDRPIISLVGVDNSAFGVIKKAVIAMRK
                     REFKLIAYLGYEDSYKKSFELYNIEADPEELDDLSDRDTATLSAMKDELLTYLDEANK
                     PFRKS"
     gene            10605..12146
                     /locus_tag="PL000_05890"
     CDS             10605..12146
                     /locus_tag="PL000_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC88460.1"
                     /translation="MAQITRRDFLKLAGGALAGSLVSSKLTSLAQGDKDSPNIIIILC
                     DALSARHMSLYGYPRETTPYISAFADRATVFHQHISGGNFTSTGTASLLTGMFGWKHR
                     AINQGGLVLPEYAKTNPYSLLSTDYFKFAFSQNPWSDRLIGQNSRAVDKFLPVTSYSL
                     REENPISRIFKNDRTLASIALDDFMIPVQGDTPGSSIISAYYKKKAMDTFLKDKNKSA
                     RYPDGLPEILIGGYVAPYLNESVYNGVYEEVSQLEASSQPYFAYFHLFSPHFPYSPRR
                     EYLKLFRDDFSPPLKPIHPLSPRLHKDFIASRRTAYDRQVAQIDEEFGRLIAKLDKLE
                     ALANSYLIFTADHGELFERGFSGHGFQFMYEGALHTPLLIHAPGQASRVDIHTPTSAV
                     DLLPTLLRITGKDIPTDIDGKVLPTFGGDEDAERPIFSVYAVDNSAFGPLKKCVIAMR
                     KGAHKLIAYLGYKNYDEVFELYNIETDPEELFDLAAKELDLLSKLKQEFKAHLEEANK
                     PFAKT"
     gene            12216..15170
                     /locus_tag="PL000_05895"
     CDS             12216..15170
                     /locus_tag="PL000_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC88461.1"
                     /translation="MTLSPDFLHELKKRFTGDLRLDPASRTLYSTDASMYQIEPLGIA
                     IPKTQDDLQAAVELAAKYAIPILPRGAGSSLGGQAIGEALILDCSRHLDSILKIDPDS
                     QTATVEPGVVLSTLNAASAKFGLTFGPDPASAERATMGGVIGNNATGAHSILYGMSAD
                     HLVSADVIMADGSLAVWNKIVLEEREKRKDDSLFSSFLSTALDIREKYSKAIKQNFPK
                     TWRNSAGYRLNYLLPWSPSAPPQWIGDSYPANLQPSIFNLATLLAGSEGTLAVMQRAT
                     VNLVKKPKHTILGVLAYQSIADACDDVPRLLEFKPSAIELIPQMIIQLARGVPSFARQ
                     MGWMTGNPAAVLVIEFSGDQPSALKEAVRRVGNVITIAESPEEQARVWNIRKMGLGIL
                     DSRPQAARPAAFIEDCAIPVERLGEFVREIERILRDHHTEGGIYAHASGGCLHIRPIL
                     NLHLGEGVRALRSIGGQVLQLALRLGGSMSSEHGDGIATGEWIEKTYGAEVTKAMRTL
                     KHAADPRNILNPKKMFDAPPMDTNLRYGENYQAKTWVPSLHLDHERGLAGAIEHCNGQ
                     GVCRKTNGVMCPSFQATRDEANSTRGRANLLRGLITDYHLPSAENARQKELMQATYQA
                     LDLCLACKGCTSECPSGVDMPKLKYEFMNEYYKTHRRHLRDYLFGYFHVVSKWLAPAA
                     PLANAFMQMSWAQKLIARTTGITEKRPFPLFAKRSSAQKAYPNAWKNGKAVIFLSDVF
                     SRYLEPEVEQAAFELLHAAGYEVKTLPIIGSGASLLSKGFVDAARKHAEKVLSEIHAL
                     DPDSTLSVVGCEPPEVYCLKHEYSALLPNRRTELESISRRVWLVDEFLLRDENLERLV
                     QMGETAPHSEVIHFHPHCHQRAEGLSEDGLPNGTAATTELLRRFGFQVNLLDTGCCGM
                     AGTFGYDANHYELSMQVGELKLFPKIRELGLGNGDLASSGSACRLQIKQGVGKTAEHP
                     LVVIAKRISPNG"
     gene            complement(15231..15671)
                     /locus_tag="PL000_05900"
     CDS             complement(15231..15671)
                     /locus_tag="PL000_05900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC88462.1"
                     /translation="MANGGEFKIELFADKAPITVNSFVFLTCKGFFNGLTFHRVLEGF
                     MAQGGDPTGSGMGGPGYEFVNEYSDLTFDKPGILAMANAGPDTNGSQFFITFGPAEWL
                     NGGYTIFGQVVSGMDVVNSLTLRDPQQNPSFIGDAMESVTISQE"
     gene            complement(15980..16462)
                     /locus_tag="PL000_05905"
     CDS             complement(15980..16462)
                     /locus_tag="PL000_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC88463.1"
                     /translation="MDEERSKPKEMKAVLLDNIRSAWNVGSILRSADGFGFSHAYLCG
                     ITPTPEVDAVRKTALGADEFVTWSHHKDSVKLVTGLKLEGWKVVALEEDERSIPISRY
                     AEFQFPSPVVMLLGSEVTGVDPELLELADAVFHIPMHGQKRSFNVAVAFGVAGFSLSS
                     "
     gene            16785..20627
                     /locus_tag="PL000_05910"
     CDS             16785..20627
                     /locus_tag="PL000_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC88464.1"
                     /translation="MFVDLSGFSTMTDALSAHGTYGAEVLANMMRVVFEPLVNAVYEQ
                     GGFVIGYAGDAFNAVFPTEQDAGQTMRRCLAAAVQMQAHAKANQLASTPYGDFSIRIK
                     VGIGYGQVTWQIFKSINGKRASYWFRGESLTGAVLGEERAYAGEIVADPISYKLISDI
                     VEVRPVQECFLIEKVIADLPAALPVPTPEPADDLASIFLADTIAHLPISGEFRQAMNL
                     FIDIPVNISDQALITPFMETVYLLQEQYGGVFLRPDLGDKGFNLLMFWGAPIAYENDV
                     DRALNFIIELATRTRITLRAGITYHMAYAGFIGSSKREDYTAYGWGINLAARMMEHAG
                     QGEYWMSEEVARRAEKHFNLNFLGEHHFKGFKQDQKVFSLLGRKNIVETVYQGQLVGR
                     EKEMENLRSFTEPIHRGNFAGVMVLQGEAGMGKSRLIHSFQFSEYFTEHPARWVVCQA
                     DEILRLPFNPFKDWLKKRFELLEGQPDEINAIAFNRNLQELANITPDPELAAELIRTR
                     SVLSALINITQPDSLYESLDAKGRYDNTLIALSALLRAESLQRPLVLFLEDTHWMDED
                     TGNFLSYFMRTLLAESEKQYPIAIITTQRPEGNANWELDGTYAHHIKLEKLPAASIAH
                     LAKNIVDHPISASLLELLNSRTDGNPFFAEQVLRYLTEHNALSLNEEGEYIANQAENI
                     SLPTDVRAVLVARLDRLTKQVKEAVQTASVLGREFEIRVLGLMLKNDKRLLSHVAQAE
                     SANIWTPLSEIEFIFRHALLRDAAYSMHLVARQRELHGLAVSAMETIYHEDLEPHYGE
                     LAYHAEKAALNEKALHYLNLAADSASQAYQNAQVIDYLTRAHNLLPQNSQHERFEVLY
                     KRSIAYSIIGNRSSEDMDLTALEETASALRDNTKLARVLMRRTYYFATQGENFMAISF
                     GERAATLAQSVNDMETLGEVYNFLPSAFTRVGSLKEAFECAQNALKLSQSTNNRLGEG
                     NALTSLGLIALENKGPSEAYQYQANALKIARELGNRDLEAKVLNNLANLIGLSNGDYS
                     TARDYFEQAQVIYQEQGRMYPSGLALTNLGWVSNMLGDYETALTYHTRALTIAREVGN
                     KTQEMYTLINLSASTFGKGNNPNAAIYWAEKALWLSEKIADQIGTAWSFFYLGYGYML
                     GKQYPAARQAFESSLKIREGLDAKVLTAESQAAIGQTLYELMDIEAAFIEVEKVLEYM
                     QENPNLEGAEEPLRVMLTCYLVLKEKRDPRHKNILQNANLLLDTQVSKLRSDEARHVY
                     VENVPWRRMLRKYYKETQ"
     gene            complement(20631..21017)
                     /locus_tag="PL000_05915"
     CDS             complement(20631..21017)
                     /locus_tag="PL000_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88465.1"
                     /translation="MQNKVVVPGKVKKDKDNNKKYKIEKDKVSESDITIDIVDDGDYD
                     VEKLSVEGLPANMVDGNPITWINNFSIKKNGQYINQRFFVTIPNIGSSKLIIFDGNGN
                     PYYYTGEIKNNRFELTDGDPAIGHWP"
     gene            complement(21134..21970)
                     /locus_tag="PL000_05920"
     CDS             complement(21134..21970)
                     /locus_tag="PL000_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88466.1"
                     /translation="MMRNSQLDSANPPISSLLRSITWLEVLVLIVTGGGLFFLPALAR
                     EQWPWVIAPFNGRFVGTVYLGSFVSAGFMAFYGRWSPGRIVLPMIFIFTTIVLGVSLF
                     HLDQFDLQRWQPWIWFLLYVVLPLNSAYHLWLYRAWAPAESTPTPATWRNYALGLSAL
                     FGLYGLALLIAPTAVSAFWPWPIDAFHGQMYSVVFITPAVGIFLITRQASKLEWKTLG
                     LTLLVIGACAIIGLLLVDAVVPPEKKVNWATAETWIWMALMTLYGASGAAMILRSGGK
                     NP"
     gene            complement(22039..22278)
                     /locus_tag="PL000_05925"
     CDS             complement(22039..22278)
                     /locus_tag="PL000_05925"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HNC88467.1"
                     /translation="MNSDRPKNIPDGITLEMIVTQLSEYYGWAELGRRIEIKCFTNDP
                     SIKSSLKFLRKTPWARKKVEDLYLDSLHKMNPRSG"
     gene            complement(22291..23103)
                     /locus_tag="PL000_05930"
     CDS             complement(22291..23103)
                     /locus_tag="PL000_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC88468.1"
                     /translation="MGKKAIVFCVIGLFVVSCAAPQSLVTSTPDVTPTVAASFTPSPV
                     PTITATAYLTPTPPAALVTSGDWTLPYVALTFDMCQDPLYPAGYDAGIVDVLQRYNVP
                     ATFFMGGDWMRTHPDETRLLGANPNFELGNHSWSHADFTTLTAEQMGQEIDMTEDMMF
                     QLTGKRSHLFRFPSGFYNDTALQIVSEHGLYAVQWDSVTGDPDPTFDAATILAEVQRT
                     VQNGSIIIMHGNGRGWHTAEALPLVIDYLQSKGYTLVTVSQLINLEPLPAAE"
     gene            complement(23153..23941)
                     /locus_tag="PL000_05935"
     CDS             complement(23153..23941)
                     /locus_tag="PL000_05935"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HNC88469.1"
                     /translation="MKTTKSLIGIFVFIAVLLVTGACSGNATTTSTPLEITPPDVSTN
                     TPTAAPVLQTTETPIVPTVDPVYFRDDFDDILQPGWTWLREIPGQWSLDKAPGSLRIA
                     GGRGSVNAETMTNILLRPAPAGDFQIETILTFEPDQNFQFAGLVVYQDNNNFIQAGQG
                     YCRGGGCVSRGLYMNYYGRGLIVPPNFAQSYKNNAVVLRLTRKATSYIFEISGDGKVF
                     YKVGAHESDMTPLQVGVLAGQNVDGISTTIPALFDYFEMSSPAP"
     gene            complement(24055..25014)
                     /locus_tag="PL000_05940"
     CDS             complement(24055..25014)
                     /locus_tag="PL000_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HNC88470.1"
                     /translation="MLRSFFIYLSKAAWAQNLITNWSFAWRAASRFVAGTNLSDAVRV
                     VKELNAKGINATLDHLGEHTNTPEEAQQATDDIFATLDAIGAEATLRGNVSIKLTQIG
                     LGLDENLCAENLERILARARQNNTFIRVDIEDTPYTDKTINLYYMMREKGYTNVGMAV
                     QSYLYRAEADTRRLTQDQITIRLVKGAYKEPPEKAFPRKADVDANYDLLTKILIDASL
                     AFQTKLSADGRFPAIPAIASHDEKRIAFAKQYADKVGLPKDGLEFQMLYGIRRDLQER
                     LVKEGYPVRVYVPFGTHWYPYFMRRLAERPANIWFFISNFFKG"
     gene            25124..26614
                     /locus_tag="PL000_05945"
     CDS             25124..26614
                     /locus_tag="PL000_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNC88471.1"
                     /translation="MRNRNTNTILRGVSILFLTAALVLSIISLITYSRQRNNYPASMT
                     IAGVPVGGLTPVEASQRLLEVYTSPVELLYNDAIIHIEPAVVGFEPDIETMLAAADLS
                     RTGGSFWGGYWDFLWNRTPSEIAIPLSPKFSEERLREYLQNEIAARYDVPPSPAVPVP
                     GGTDFLPGQPGQTLDLERAVLLVNDALRSPTNRSVALTFTRGSVARPTIDNLTILLKQ
                     IVQVSDFDGLIGLYMMDLQTGQEIHFAINNKEEISVTPDIAFTASSTMKIPVVASYLI
                     NRGSNLSPDIANSISQTLGKSSNDATDVVLSAIEPNRGPVIVTENMRSIGLESTFLAG
                     FFTSPAYLLPAIPITPANSRTDVTTEPDSYSQTTPSEMGSLLADIYQCAQSNGGALIA
                     AFPDKVSPQTCQLLIDFMAQDKLGSLIQGGVPDGTLVPHKHGYVPAADGVVHDTSDVG
                     IVYTPGGNFVLSVYTYHPVTNIWDITNPLIGDITKAIYNYFNVTSQ"
     gene            26700..27398
                     /locus_tag="PL000_05950"
     CDS             26700..27398
                     /locus_tag="PL000_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88472.1"
                     /translation="MSASLGLFRLQQVDRKIDRARSQLETIRKTLENDTELRECLTHA
                     ETAKASHHHAHHNLKSAEMEVETQKIKIEQADSSLYGGRVQNPKELQDLQKEIVSLKK
                     HLATLEERELEAMLAAETAEQNLQAASTQLELIQARLGSEHTKLLADQNALVAELEQL
                     IEEREATLSPLDTSLLQIYEDLRQQKRGIAVTEITDNSCAACGGTLNSAIQQSARSQK
                     LANCPTCGRILYSN"
     gene            27408..29957
                     /locus_tag="PL000_05955"
     CDS             27408..29957
                     /locus_tag="PL000_05955"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC88473.1"
                     /translation="MSRFAIDPYSFFVGFIAASIFWLLVSRARPLWAELVAGIKEQRE
                     IAQTRKSSTVEENHRRITLRRAQGMHLAAPLFSLDEIILEPLLIAPPQVVQPGTPAKF
                     EDVVSQTLPYMPMWPEIAAAYHAQTLTLTQALSGNANIVIMGQPGVGKTVALAHLASL
                     AANRSAKLETLQNAVPFLYHVANLKLPLPDPKKDVLAPLIEAASENAPLMDLNRLPGF
                     FEGAFKSGEALLLIDGFDEITAEYQQLVTDYFKAIMQAYPKTRIVTTGAPEYLDGLIP
                     LGFAPLALMTWSQRENQKFIEHWGELWSQTVAMEAWAQTGPEQVDPLLLNTWLGTDNL
                     NLSPLELTLKAWGAYAGDSLGPHVLEAIATHIRRIAPANTPLAALETLAMQAMVNTQP
                     IFDPRVARAWVKSFEVAEETPTGEATEQAAAGEETEEKAEDTKQLKGKKVEKVSTPTS
                     GLLGKMAASGLLVVYPNNKMRFTHSVFAGFLAGRALMSFKADETILNQADWSGKYLVM
                     RYFAAHGDASKLVQSLMEFSRLPMHRPLFAAARWLRDAPRNAPWRGKLFGTLAAIMQS
                     EGLPLNLRGQAMAAFVVSNDSSITTLFRQLAGTLSFELVHLAVLGAGAMRDAKALEVL
                     ERALQAPSTAVRRAACMALVSVGTNDALETVAHTLLSGEEDIRRAAAEALANDPHEGY
                     AMLRDGVTIDDILLRRAAAYGLSRVSEEWAIELLKKIQIDDKEWVVRNAATEVLDSKT
                     SAGSLAPHKLKAPSESPWLIEFAAKQGMGISPGVPATDILLLALKSDDAETRLAALPY
                     LKYTPNEGVISQLYAAMYKDDHELREAAYNTLWELGASGVKLPDPSQYGFG"
     gene            29960..31312
                     /gene="ssnA"
                     /locus_tag="PL000_05960"
     CDS             29960..31312
                     /gene="ssnA"
                     /locus_tag="PL000_05960"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNC88474.1"
                     /translation="MLITNAKIITWETTNQVLENHAILIEGDRIKEIGTTKALTAKYP
                     KAKTLDARGQYVMPGSICAHTHFYGAFARGMAIPGPAPKDFPEILQKLWWPLDRSLDA
                     EAVRFSALVCLVDAIKHGTTTLIDHHASPNAIDGSLDIIADAVDKSGLRGVLCYEVTD
                     RDGSEKANAGINENIRFLKRLASNPHPRLAGTFGLHASLTLSGITLQACRAAVPEGTG
                     FHVHTAEHESDEYDSLGKSQMRVIDRLQKHGILGPNTITAHGIHFDAREMEILKETGT
                     WLTHQPRSNMNNGVGVAPVESMLRLGVKVCLGNDGFSNAMWEEWKAAYLLHKIHHRDP
                     RRMGGYDVTQMAVYNNAALANQFFPAAPIGQITQGAFADLIFVDYHPYTPLTAGNLPW
                     QIIFGFQQSMVTTTIAAGKVLMKDRELLTLDEAEIAARAREIAPRIWKRYEEEVAKVM
                     "
     gene            31349..32029
                     /gene="npdG"
                     /locus_tag="PL000_05965"
     CDS             31349..32029
                     /gene="npdG"
                     /locus_tag="PL000_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNC88475.1"
                     /translation="MNDSNLLLSIAVLGGTGKEGKGLAFRWARAGYHVHIGSRSAEKA
                     IAAANEIREMLGDGISISGMSNPEAARDSNIIVLTVPYSAHRDTLESVKEELKGKLLV
                     DVTVPLVPPKVATVQMPAAGSAAQEAKQIVGEEVQVCAAFQNISHEHLLDNSEVECDV
                     LVTGTSKEARAETIKLVEAAGLIGWDAGPLENSVVVEGLTSVLIGINKKYGSTHAGIK
                     ITGAKRNT"
     gene            32084..32851
                     /gene="cofE"
                     /locus_tag="PL000_05970"
     CDS             32084..32851
                     /gene="cofE"
                     /locus_tag="PL000_05970"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNC88476.1"
                     /translation="MSLSITPLQNIPLIRQGDNLADILVASIQNSTIQLENDDILVLA
                     QKIVSKSEGRMVNLATVTPSSQALELAQKTEKDPRVVQLMLQESNEIVRTRIGAIIVE
                     HKLGFVCANAGIDHSNVAGDGSENEEYVLLLPEDPDASAKALRDQIRQKTGLNVGVMI
                     IDSHGRAWRNGTVGICIGLSGIPAMIDERGWKDLFGYTLKITVVGVADELAAAASLMM
                     GQASEGIPAVHVRGFPYPLGEGSLKELIRPKEQDMFR"
     gene            32907..33299
                     /locus_tag="PL000_05975"
     CDS             32907..33299
                     /locus_tag="PL000_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HNC88477.1"
                     /translation="MKNFPPEYLDLLKDETRAYLFLATTMSDGSPQVTPVWFNMDDEY
                     ILINTNEGRTKDRNMKARPKVAFVIQDPETPYRYIQVRGEVAEFTTQGADEHINQLSL
                     KYDDRPWKYREGQKRIIFKIRPTHFDRH"
     gene            33241..33915
                     /locus_tag="PL000_05980"
     CDS             33241..33915
                     /locus_tag="PL000_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC88478.1"
                     /translation="MKDKSGSSSKSDQLTLTATDLHTFLRTRRSIRRFKTDPVPDTVI
                     ESILSTATFSPSAHNRQPWRFAVVTASAVKEKLADAMAVEFQRDLEADAISPENVQRK
                     VSRSRERIVAAPVVIILSVDMTDMDTYPDTRRKKAEYIMATQSTANAGMQMLLAAHAE
                     GLGSVWVCSPLFAQETVQNVLGLPRSWEPQAMYFLGYPSETPEVRERKSIKEIVKRVD
                     VQKSTP"
     gene            33926..34831
                     /gene="cofD"
                     /locus_tag="PL000_05985"
     CDS             33926..34831
                     /gene="cofD"
                     /locus_tag="PL000_05985"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HNC88479.1"
                     /translation="MKITALAGGVGGAKLAHGLAQILPSQDLTIIVNTGDDFEHLGLS
                     ICPDLDTVCYTLAGLANPETGWGRVNETWNTIANIEKLGGPNWFRLGDQDIATHLERT
                     RRLKGGETLSQITKDFCKAWGIQPTILPMTDAPVRTIVDSDEGELAFQEYFVHRQCGP
                     KVKGFRFDGVEAAEPAFGVREAVESADAIIICPSNPWVSVDPILRVIPLPKKKIVAVS
                     PIIGGKTVKGPAAKMYAELGIEPSALAVAEHYRDLLSGFVLDNVDAHLSDKIRTRTLV
                     TDTLMNSLTDRARLAMDVLHFIGSL"
     gene            34834..35439
                     /gene="cofC"
                     /locus_tag="PL000_05990"
     CDS             34834..35439
                     /gene="cofC"
                     /locus_tag="PL000_05990"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HNC88480.1"
                     /translation="MTLWAIVPVKPLRRGKSRLAGMLTEDERTALNQELLERTLKTLS
                     TLKELDQVLVVSRDPAALTIARNHGAKTVQEDGQPHLNTALMRATVVAQVHATQGVLI
                     LPADLPLLTPDDVLTLIDRAAKPPVVVIAPDRHGKGTNALLMVPAGQIEYDFGEGSFQ
                     RHCERTKQSGARLEIVELPSLGLDLDLPEDLEMVRKMEALK"
     gene            35505..36503
                     /locus_tag="PL000_05995"
     CDS             35505..36503
                     /locus_tag="PL000_05995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC88481.1"
                     /translation="MTERVALYLQDSHDLRDGLDYAKYAEEKGFEAVWQAESRLVRDA
                     IVPMAGYAAVTNKIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRVICGLGAWWDPLA
                     KNVGIDRRKPLTAMKETVIVMKKLLNMERVSFDGEFIHVNGIELDVVYGRREPRNVPI
                     MIGATGDQMMELTGEVADGAVLNYCVAPEYNDRAMELLEKGLRKSDRKMENFDRPQLI
                     VCSVDEDHDKAINYSKMLLCQYIAQQPHIAKASNVSDDVVHQIQSILGWPATKEQVMK
                     AKDLVPDELVHKITASGTPSEARAKVAEYTKRGCTCPILYPVGGNFKLLIDTFAQS"
     gene            36609..37223
                     /locus_tag="PL000_06000"
     CDS             36609..37223
                     /locus_tag="PL000_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC88482.1"
                     /translation="MKPNSPTLSPQETRERILEAAVTVFASKGYHDTKVDDIVSESNT
                     SKGSFYFHFPSKQDIFLALVDTFADLLETRLKERLSASTSGLERVELALRVCLETFGQ
                     YRALAKIALVQATGLGAAFEEKRRTVNERFIQFIKENLDAAVADGSIPALDTEVTASA
                     WIGALNEVVMRWIYTGQPDPERSLPALRKIFLLSLEIPIERINQ"
     gene            37306..38262
                     /locus_tag="PL000_06005"
     CDS             37306..38262
                     /locus_tag="PL000_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC88483.1"
                     /translation="MRRIRSLLAFVIIALLAAACAPATTAPQETVTLKIAVLPIIDTL
                     PMYVAQQEGLFAKHGVNVEFVPVASAPERDQLVGAGQADGMVNETISVMLFNKEKVQV
                     QAVRYALRPTENAGHFFIIASAKSGITDAQGLKGIEIGVSQGTIIEYVTERLLQAEGL
                     TSDEIKTIAVPKIPDRMALLASGELNAGVLPDPLGALAVQQGAVIVLDDSRHPEYGFS
                     VISFRKEVIDANPAAIKAFLAAIEEATTMLNSDSKKYTSVLSDQKLVPPPLLASYAVP
                     PFPTAGVPTEAEWSDALAWVKEKGMLTTDVSYADSVNATFLP"
     gene            38333..39088
                     /locus_tag="PL000_06010"
     CDS             38333..39088
                     /locus_tag="PL000_06010"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88484.1"
                     /translation="MITVSSLTFNYANNTPIFQDFNWQVQRGETWAILGSSGCGKTTL
                     LYLLAGLRFPSKGQIEIDGERLTRPRPHSGLILQDYGLLPWATVQENAGLGLKVREFY
                     GEDGKHAPKNFQPENNVAHWLTRLGLSEVADKYPSQISGGQRQRTAIARTLALSPDLL
                     LMDEPFSSLDAITREDLQNLTLSLCEEQKLTLVIVTHSIEEAASLGGKILLLGSSPRV
                     FENPTVRQGDHRNSQEYRDLCNLLWKEMRNETA"
     gene            39075..39815
                     /locus_tag="PL000_06015"
     CDS             39075..39815
                     /locus_tag="PL000_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88485.1"
                     /translation="MKRRDLLFSAAGLVLIWQVAAMLIQRPILPAPWEVLVVFVQELG
                     NGLVTHFFYSLWRVTAGMALSVLIAAPVGLAVGGSKRLDAIFSPIIYLLYPIPKVVFV
                     PVVLLFLGIGDVAKITIIFLILFFQIVVLVRDQAAGLAPQLIQSLQSLGAGRRALFRF
                     VYLPASLPAILTALRQSIGTAVAVLYITELFATKYGLGYYIYYNGSTLFHYPAMYAGV
                     IAMSLLGLGMYYTVDWLERKWCKWKFVS"
BASE COUNT        10033 a        10853 c         9780 g         9154 t
ORIGIN      
        1 ttttcgtaaa ccgcatccgc cactcaaaca gtttattcaa tgcctcaatt ggcgaaagtg
       61 aattcacatc caattccttc aactcatcca aaagcggatt cgtctcaggg aaaagcgcca
      121 cttgttgcgc ggcattgggg tcaattttca cagcgcggcc tgatgtcttt tcaagctcag
      181 ccatgatctc tgtggcacgt tggatcactg gtgcaggcaa ccctgcaagc tgtccgacgt
      241 ggattccata cgaacggtct gcaccgcctg cgatgatctt gtgcaggaac acaaccttgt
      301 tgtcagcctc acttactgcc acgttgtagt tgcgaatccc aggcagcagg tcggcgagtt
      361 gggtcaactc atgataatgc gtagcaaaca gggttttcgc acgcaattgc ggatgattgt
      421 gaatatactc aataatgccc cacgcaatgg aaagcccatc ataggttgaa gtgccacggc
      481 ctatctcatc aagaatcaac aagctgcgcg gcgtggcgtg atgcagaata tttgccgctt
      541 ccaccatctc caccataaat gtagactgcc ccgcatgaat ttcatcctgt gcgccgatgc
      601 gggtgaagat tcgatccacc aacccgatct ttgcagatgc ggcaggcacg aacgaaccca
      661 tttgcgccat caacacgatc aacgcggtct gccgcaaata ggttgattta cccgacatgt
      721 tcggccccgt gatcacccgc accacttcac ctttttcgaa gatgacatca ttggggatgt
      781 acctgtctga tttcaacaac tgttccacaa caggatgcct cccctcatgg atctccaatc
      841 cgcttccttc gtggacctcg ggtctggtgt agcctccgag agcagcagcc tcccccaaag
      901 cagacagcac atccaattcg gcgattgccc gggcagtggc aaggatttga ttcgcggcct
      961 tcaccaattc agcacacacc tcacggaaca aacgtgtttc gatctccttg atccgttcct
     1021 cggcgtttag aaccagcgtt tcgtactcct tcatttcagg cgtgataaac cgttctgcat
     1081 tgaccagtgt ctgtttgcgg atgtaactct caggggcctt atccgctgcg ccgcgtgaaa
     1141 tctcgatgta atagccaaac actttgttgt agccgacctt gagggttttg atgcctgttt
     1201 tctcgcgctc cacgccttcg aggttggcaa tccaatcacg ggcgtgcttt gaagcgtcta
     1261 tgacgccatc caattcagaa gaatagccag ggcggatcac acccgtattt tgcagcgtag
     1321 caggcgggtc atcgtcaatg gcgttttgca gcagagaaag ctcctgggta cacacagacc
     1381 attgatcgct gaccactgct ttttgtccgc tgatgacttc ttttatcttt ggcaactcgg
     1441 tcagagtggt gcgcatcgca accagatcac gcggttgagc ctgccctgcc aacacacggt
     1501 tgaccagccg ctccaggtct gcaagtggct ttagtgtcgc ccgcaattcc gcacggacca
     1561 tgccattttc cacaaaatac tgcacgccgt tctgccgttg tgtgatcttc gccacatgca
     1621 gcaaaggctg actcacccac tgatgcatga gacgctttcc catcggcgtc accgagaaat
     1681 ccagcgtacc cagcaaagac cctttgcgca tgccccgtgt ggggttggcg gggtcgccgc
     1741 gcaaggtttc atccaattcc aaatttctgc gcgtggaggc atccaacgtc atgaattcat
     1801 tcaggctgta ggcacgcagc gaagtaagca aattcaatgc atcgggctgg gtttctttga
     1861 ggtattgcac aatggcaccc gccgcccttg cggagaggct gttatctttc aacccaaatc
     1921 cagccaaggt ggaggccttg aaatgtgaga tcaaaacctc gctgcatttg cctggctcga
     1981 acttccatgc aggcagggcg gtgatgtgac ctgtgatccc atcgggtaaa acttgcgaat
     2041 cggggtgaat gatctcagca gggtgaagac gggtcaactc ggcacgcagg gcttcaagcg
     2101 gcaattcggt aacggcgaat tctccagtgg tgatatctac ataggaaaca gaagcagtga
     2161 tcgggttgga attgaccgct gttgtcgtgt cgagaatgac cgaggcaagg taattattcg
     2221 catcgccggg cagcaatcct ggttcggtca ccgttcctgg tgtaaccaca cgcacaacct
     2281 tgcgcggaaa aatccccttg acgggttgtt cgccaacctg ctcacagatg gcaacgtgat
     2341 aacctttttc gatgagacgt gccagataat tttcaaccgc atggtatgga atgccagcca
     2401 acggcacacg cacgccgccg cccacggggc gcgaggtgag aacgatatcg agttcgcggg
     2461 ctgtgatctc ggcatcttca tcgaaagtct cgtaaaaatc gccgaggcga aagaaaacga
     2521 ttcggtcggg gtgctcgcgt ttgatctcaa gatattgctg gcgtacgggg gtcaggtcgt
     2581 cgttactggg catgggcgct gctctgaatt aagtttaatg tgtgatttga cttattatta
     2641 taactggagt ataagccttt attccaaccc taaatacaac ccatccatat ctcaaaacgg
     2701 cccatgttca atcaccgact tccaaaacta aacgatgtac tgtctgtgta cgcggtcatt
     2761 gcaaccatgc tcttcgcgtg gtcggctttg ctgttctttt ggtacctgcc atcctggatg
     2821 cattttatgt tgctcggcga aattgcggcc actttttcgt acgtggtcat tgcaagtttt
     2881 ttggagggat taagctttct gctctttttg ctggtgctgt gtttttttct gccgcccgaa
     2941 tatctgcgtg atgaattcgc cgcgagaggg acagccctca ccctgcccat catcggcatg
     3001 atcatgatct acttccgcat tacgaatgag aacattgcac gcatcatctt ttcggttaca
     3061 gggttggtca tttcgctggt ggtcatactc ctgttttatt tttcagcgaa caacccgcgg
     3121 ataaaatcag cttttgcggc gctggctgag cggtttacgg tttttctata catcttgatt
     3181 ccgatctttg ttttatcgct gctgggaatc gccttgcgaa atatcttcta agagggaaag
     3241 atgaaaagtt taagcagacg tgactttttg aaaactgccg cggccttttc ggctggctcg
     3301 ctgctatcca tggctcctgc gcttcaaaac atcaaagcgc aaagcaacaa gccgaatatc
     3361 atcgtcatgg tctttgatgc catgtcagcg cgcaacctgt cggtgtatgg ctacccgcgc
     3421 agcaccaccc ccgggcttga gcggatcgcc gaacgggcaa cggtctacca ctcgcattat
     3481 tcggcaggga atttcaccac ggctggcacg gcttccatgt taactggctt gtatccatgg
     3541 acacatcgcg gaatcaattt gagcggcatg gtcaacaaga atcacgttga tcacaatatt
     3601 ttcaacctca tcggggatga atatcatcgc actgcattca cgcaaaatta tcttgccgat
     3661 gttctgcttg ggcaattcca atcaggtctg gatcgtcaca tccctgcgcc aactttttac
     3721 gaccaattgg atcatccgtt gatcgggcaa tactttcccc gagacccgat ccctgcttat
     3781 tacgggctgg atgattacct tttttctggc ggcattgacc gtgtgggcag cattccttcc
     3841 gcgcccattt ttggtttttt gaacaaattg gcgcgcagga atcaggactt caacaaatca
     3901 ctgcctgact atccgtatgg gattccaaac aacacgttct atagctacaa acatgaagtt
     3961 gtgtacgcgg gcgtgctgga tgagatcaag aatctggcca gcgaacaaaa cccgtttctg
     4021 gcgtactttc acctgttctc accacatgag ccgtatcggc cccgtaaaca attcaccgat
     4081 atcttccctg agttggaagt cccattcaaa gcgccgacga aattctccac actggatatt
     4141 gacaacgacc atatcctctg gcgccgcaag acctacgatg aattcgtggc ggatgtggat
     4201 tctgagatcg aaaaactcgt gaacgcgctg gacgaatcgg gtattctgga aaatacctat
     4261 ctaattctga ccgccgacca cggagaatta ttcgaacgcg gagagtatgg tcatgtcacc
     4321 cgattgctgt acgatgcagt ggtgcatatt ccgctcatcg tcattgcccc aggacaaaaa
     4381 tctcgtgtgg acatccacca ccccaccagc aacattgacc tgctgccgac gctgctgcat
     4441 atcgctggca aaacggccgc aactgacctc gaaggcagaa tcctgccagg gctgggcgga
     4501 atcgaagagt cacgcagttt gttttcgatg gaagccaagg aatgttccgc atttggaaat
     4561 ctcaaagatg gtgctactgt ttcgctggtc caaggcgagc acaaactgat ctactacact
     4621 ggctacccga aacgcccaga cacattcgaa ctctacaacc tgaaagatga ccccgatgag
     4681 atgaaagacc tgttcacggt tgacaccacc actgctgcaa aaatgaagga agaaatgctg
     4741 gatacttttg aatccaacag gaaattaaaa tagtctaaac ctctcaaatg gacaagggtt
     4801 gtgatgtcag atataatctt caaccgaaaa aaggagtgtc tcaaatgtat attgacccaa
     4861 acacaggcgg catcttattc caggctcttg ccgcagtatt cgtagcaggc tcgggcgtat
     4921 tgttcttctt ctcccgcaac attcgtgaag gtatcgcaaa gctccgccgc cgtttccgca
     4981 aggaagacga agagcaaaat cagtaattcg gctgcatgaa gacgatcatt cttggctttg
     5041 actcttttga ccccgctgtg ttcgaagaca tggcggggca aaataaatta ccgaactttt
     5101 cgaaattcat ggacgccggg gggtattcac gtcttgaagt atgttcacca ccacaaaccg
     5161 aagtttcatg gacgagcatt gccacggggg cagaccccgg ggggcacggc atttttgatt
     5221 tcgtgcatcg tgacccagcc acatacgccc catatgtttc gatcctgccg atgaaaaaaa
     5281 gcgcgatggg agagcaattt gtccctccat acatcgcgaa gacccttttt gaagaagccg
     5341 ccagcatggg ctacccagcc actgctttat ggtggcctgc catgttccca gcacggcccg
     5401 aattgcccgt caacacactg cccggcctcg gcactcccga tatccgcgga cagttgggag
     5461 tcggcacgct tttcaccacc gaggatgaaa agaaaagcaa gactgcggta acgcgttttg
     5521 aatccacggg caagggcagg tactctgctc tgttgaatgg accgcaagtg caaggcaaga
     5581 acggtccgca gcctgccacg ttgccgatcc agcttgaagc gctggaccag acttcggcac
     5641 gggtaacgat cggcgatcag cagatccaac tccgcgttgg tgagtggagc gagatcgtgg
     5701 aagttaaatt caaagcgggg ttattcctga gcattcacgc catcacccgc atgatcctca
     5761 ccagcttgaa cggtgttgtg cgtttgtaca ccctgccgtt gcaaattcat ccactgcacg
     5821 cgttatctca ttattcttct tcgaaatcat ttgcgaaaga cctgtggcag aaagtggggc
     5881 cgtatctcac tttgggttgg ccgcaggaca ctactggtct tgaagatggc tgcatcaccg
     5941 atgaacagtt cattgccttg tgcgacctga tcttcgagcg ccgcaaacaa attttcttcc
     6001 atttgcttgg caacttcaaa gagggcgtgc ttggcagcat cttcgatgac ctagaccgtg
     6061 ttcagcacat gttctttcat aaccgtctcg acgtggcgca ggcctggtac caaaaactgg
     6121 atgcgtttgt cggcgaagtc agtcaacgcg tggaaaaatg gggcggtaaa tacaactacc
     6181 tcatcatgtc tgaccatggc ttcacgacct accagcaaaa gattcatctc aatcgttggc
     6241 tggctgaaaa tgggtacctc gccacaagca atggcagcgc cgaaggcgac ctatccaaca
     6301 tggactggca aaagaccaaa gcctacgcag tcggcttgaa cagcatctac ttgaatgtgg
     6361 ctgggcgcga gggcaaaggc gtggtcggcg cgaacgagat cgaatccctg ctggctgaaa
     6421 tccaacagaa attgatgggt tggaatggcc ccgatggaag acccgtcatg cagcgtgtgc
     6481 ggttcaaaca cgaagtgtac aacggagcct acacccgcct cggccctgac ctggtcattg
     6541 ggtacgcacc tgggtatcgc gcctcctccg aaacaggact cggcaaagtg ccgcaaagtt
     6601 tagtcgaagc caatcacgat cattggggtg ccgaccactg tgtggattcg gatgtcgtcc
     6661 ctggtgtgat cttcgccaac cgcgacctgc aaaactttgg cggcgtttcc ttccgcgata
     6721 ttcccttcct cgccattggt aaacacctcg accaatcaca catcaaaccg ccgtcgcaag
     6781 tcagcggtgg cagccaaaaa gacctggaag accgtttgaa gggtctcggc tatctttaga
     6841 tcattcgtag tagcgagtat tactgatcaa taaatagacc tggcaatccg tagtcggttt
     6901 tgagccattg agatgcggag ttttagcggc aaaacaggtt tgctggatta atttagaaaa
     6961 gtacaaaagt cgctgctata ttcaggtaat cctttgaaaa atctattctc acgccaaaca
     7021 ccgaaaaaga ccaatgaaaa gatcgtcgtg gtttctggtc tgccccgctc gggcacatcc
     7081 atgatgatga aaatgctcgc tgaaggcggg ttgcccaccg ttgtggattc acttcgtcag
     7141 gccgatgaag acaaccccaa tggttacttt gagatcgagc aggtcaaggc gctcaaagac
     7201 ggcgaaaaga actggatcta tgatgcgcgc ggcaaggttg tcaaagtgat ttcgtatttg
     7261 cttgaatacc tgccgaacga cctggaattc aacgtaatct tcatggacag ggaaatccac
     7321 gaagtgctgg cttctcaaaa gaagatgctt gaacacagaa atgaagtttc cgccatttca
     7381 gatgaagata tggaaaagca atatcgcgag catttgaaag ccgtccgtta ctggcttcca
     7441 cgccagccca atatgcgcgt gctgtacgtg aaatacgacc aaatggtcaa atcgccgcaa
     7501 acgcttgtgc catccatcat tgaatttctt gagcgcccgc tcaacgcaga agccatggca
     7561 gctgtcccga gcccaaccct ctaccgcaac cgcaaaccat aacctgcaat gcccgacctg
     7621 taacatgcaa tcttaaattc atggagtcca tcatgtataa acttgttctc gtccgtcacg
     7681 gacaaagcac ctggaatctc gaaaaccgct tcactggctg gaccgatgtt gaccttaccg
     7741 accttggccg ctccgaagcc cgcgaagcag gcaaacttct gcgtgaaggc ggctatgatt
     7801 tcgatatcgc ctacacctcc gtattgaaac gcgccatcaa aacactcggc ctcgttcagg
     7861 aagaaatgga ccgcgagtgg ctgcccgtgg ttcgcgcctg gcaattgaac gaacgtcact
     7921 atggttcttt gcagggcttg aacaaatctg agatggctga aaaattcggc gaggagcagg
     7981 tcaagatctg gcgacgttcc tacgacgtgc cacccccagc cctggaactg accgacgaac
     8041 gccacccgaa atttgaccgc cgctacgctt ctttgactcc cgaacaactg ccagccaccg
     8101 agtcgcttaa gatcaccctt gaccgtgttc tccccttctg gcattccacg cttgcgcctg
     8161 agatcaaatc gggcaaacgt gtgctgatcg tggcgcacgg caattccatc cgcgcgatgg
     8221 tgaagtacct cgataacatc tcagaagccg agatcaccga actcaacatt cccactggcc
     8281 tgccgctcgt ctacgagttg gatgaagccc tcaaacccat caagcactac tatctcggcg
     8341 atgcagaaga agccgcaaag aaagccgccg cagtggcaaa tcagggcaaa gccaagtaaa
     8401 acccgtttta aaactaaaaa tcaaagcagg catggatgga gcacaacacc tgtccatgcc
     8461 ttttttatcc agtcagcgag agttagccca tgaaactact gaagctcatt tctgaacgaa
     8521 tgccagactc gaaaaacatt ttggcggtgt atgccttcat cgtcatgttg gtctactcgt
     8581 ggaccctgtt cacatcgttt tacaaactgc cttcgtggct gttctacctt tccattgggc
     8641 aaattctttc ggtgtacgcc tacgcgtttt cttttgacct tctcgaaagc ctgattgcat
     8701 tgacggtcat cctgcttttg gattacacgg ttttcctctt cttgaaaaac aaggaagagt
     8761 ttcagtcgcg gtccatgatt ctggcgggta ttttgctggg cacatccatg acgcgactgg
     8821 cgttattcca aaattatgaa gatatcaccg cctttttgag cggcgaattg atctggtggg
     8881 ccattgccat tccgctgggg ttggtcctcg ctgccttcgc gccgagaatc aaattggtca
     8941 ggaccatttt tgagggattc gcagagcgcg ccacggtctt cgtctacatt tacattccgc
     9001 tgagtctgat ctcactggtt gtggttttga tccgcaacat caactaggtg aaccgatgac
     9061 aaacttttcc cgcagggatt tcctcaaact cctcggagcg gcatcggtcg gcgcgcttgc
     9121 ctctgaacgc gtgctttcgc tcaaagccgc cgacaacagc aagccgaaca tcatcatcat
     9181 tttatgggat gcgttttctg caagacattt atcgttgtac ggctatccac gccagaccac
     9241 tcccaacatt gacgcctttg ccgaatattc caccgtatat cacaaccact acagcggcgg
     9301 aaacttcacc acctgcggca ctgcctccat gttgacaggc atgatcccct ggaagcatcg
     9361 cgccatcaac tacgatgggc tggtacgtgc agagtacatc cacaacaatc catacaccct
     9421 gcttggttcc gattacaagc gattgatgtt tgcacaaaac ccctggcccg atcgtttggt
     9481 gggacagtat tacgaagatg tagaccgctt ccttccgccc accgctttca gcctgctcga
     9541 ggattcgccc gtcttttttg aaaatgatcg cgccattgca tctgtggcga tgagcagttt
     9601 tctgatgcca atccaaggcg ggaacgcccc cgtgggttca tccattctgg gttacatgaa
     9661 caaaagcaga gttttgaact tcgctgatgg gcaaaaatct ccacgctacc cgaagggcat
     9721 tcccgaagcg atgaatatgg gctacatgat cccctatctc aatgaggatg tgtatgaagg
     9781 gttgttctca gaaatgaccc agcttgaaaa gaatggcgac ccttttttct catactttca
     9841 tttgtattcg ccgcactacc cctaccgtcc gcgcaatgat tatttgaaga tgttccgtga
     9901 tgacggtttt gcccctgtct caaaaccgat tcatccgcta tcggtcggcc tgcagcagga
     9961 ttacctcaac ggccaaaggg acatgtatga caggcagatc gcccagatcg atgaagaatt
    10021 cggcaggctg attccacgcc ttgaggaaag cggcgtgctt gagaacagtt acatcatttt
    10081 taccgccgac cacggtgaat tgttcgaaag aggctttgca gggcatggat tccaattcat
    10141 gtacgagtcg gtcctgcata ttccgctcat catccacgcc ccagggcaga caaaacggca
    10201 ggatattcac gccctcacca gcaacacaga catccttccg accatcctgt ctattgcagg
    10261 caaggaaacc acaccagaca tggatgggcg catcctgcct gggctgggcg gcgaagtgga
    10321 cgaagaccgc cccatcatct ccctcgtcgg cgtggacaac tccgcatttg gtgtcatcaa
    10381 aaaggccgtc atcgcaatgc ggaaacgcga attcaaactg atcgcatacc tgggctacga
    10441 agacagttac aaaaagtcat ttgagctgta caacatcgag gccgaccccg aagagctcga
    10501 cgacctctca gaccgcgata ccgccaccct ttctgccatg aaggatgaat tattgaccta
    10561 tctcgacgaa gccaataaac cgttcaggaa gagctagcga ttccatggca cagatcacga
    10621 gaagagactt tctaaaattg gcgggcggtg cgctggcggg gtcgctggtt tccagtaaat
    10681 tgacttcgct tgcacagggc gacaaagact ctcccaacat catcatcatt ctttgtgatg
    10741 ccttatccgc acggcacatg tcgttgtacg ggtatccgcg tgaaacgaca ccgtacatca
    10801 gcgcctttgc cgaccgcgcc acggtcttcc atcaacacat cagcggcgga aatttcacca
    10861 gcacaggcac agcctcgctg ttgacgggca tgtttggttg gaagcatcgc gccatcaatc
    10921 aaggcggatt ggtgctgccc gaatacgcaa aaacgaatcc ctattctctt ctcagcacag
    10981 attatttcaa attcgctttc tcccaaaacc cgtggtcaga ccgtttgatc gggcagaaca
    11041 gccgcgccgt ggataaattc ctgcctgtca cctcatacag tctgcgcgag gaaaatccga
    11101 tctcgcgaat tttcaaaaat gaccgcacac tggcctcgat cgccctggat gactttatga
    11161 tcccagtgca gggcgacacc ccaggctcat ccatcatcag tgcctactat aaaaagaaag
    11221 caatggacac tttcttgaaa gacaagaaca agtccgcacg ctaccctgat ggacttcctg
    11281 aaattctcat cggcggatat gtggcgccat atttgaacga gagtgtgtac aacggtgtgt
    11341 acgaagaagt ctcacaactt gaagcgagtt cacaacctta ttttgcctac ttccatttat
    11401 tctcgccgca ttttccgtac agcccgcgca gagaatatct gaaattattc cgcgatgatt
    11461 tttcgccgcc gctcaaaccc attcatccgc tttcacccag gctgcacaaa gattttattg
    11521 cttcacgcag aaccgcctac gaccgacagg tggcgcagat cgatgaggaa ttcggcaggc
    11581 tgatcgccaa actcgacaag ctcgaagccc tcgccaacag ttacctgatc ttcaccgccg
    11641 atcatggcga attgttcgag cgcggctttt cagggcacgg gtttcaattt atgtacgaag
    11701 gcgctctgca cacgccctta ttgatccacg cgcctgggca agcctcccgc gtagacatcc
    11761 acacccccac cagcgcagta gatttgctgc ccaccctgct ccgcatcaca ggcaaagaca
    11821 tcccaacaga tatcgatggc aaggttctgc caacgtttgg cggtgatgaa gacgccgagc
    11881 gccccatctt ctccgtgtat gcggtggaca actcggcttt tggtccgctg aaaaaatgtg
    11941 tcatcgccat gagaaagggc gcacacaaac tcatcgcgta tctgggttac aaaaattacg
    12001 atgaggtctt cgagctgtac aacatcgaaa ccgaccccga agaattgttc gacctcgccg
    12061 ccaaagaatt ggacctgctt tcgaagttga agcaggaatt caaggcccac ctcgaagagg
    12121 cgaacaaacc gtttgctaaa acataagtcg tttcatcaaa atagaagcaa cgtgaacgtt
    12181 gcatcacgaa atcaagtctg cgatagaatc accgcatgac cctctcccct gattttcttc
    12241 atgaactaaa aaagcgcttc actggcgacc tgcggcttga tcctgcgtca cgaaccttat
    12301 attccaccga cgcatccatg taccagatcg agccgttggg gattgcaatt cccaaaacgc
    12361 aggacgatct acaagccgct gttgagctcg cggcgaaata tgccattccc attttgccgc
    12421 gcggtgcagg gtcatccctc ggcgggcagg ccatcggcga agccctgatc ctggattgct
    12481 cacgccatct ggattccatc ctcaaaattg accctgactc gcaaactgcg actgtggaac
    12541 caggcgtcgt tctttccacg ctgaatgctg cctctgccaa atttggcctg accttcggcc
    12601 ccgaccctgc ttctgccgaa cgcgccacga tgggcggggt cattggcaat aacgccacgg
    12661 gtgcgcattc cattctgtac ggcatgtctg cagaccattt ggtttcagcg gacgtgatca
    12721 tggcagatgg gagtcttgca gtttggaaca aaatcgtttt ggaggaaagg gaaaagagga
    12781 aagacgattc acttttttcc tctttcctct ccacagcact tgatattcgc gaaaaatact
    12841 caaaggctat caaacaaaac ttccccaaaa cctggcgcaa ttcggcgggt tacaggctga
    12901 attatctttt gccgtggagt ccatccgccc cgccgcagtg gatcggcgat tcatatccag
    12961 cgaacctgca accttccatc ttcaaccttg caacgcttct cgctggttcc gaaggcacgc
    13021 tggcggtgat gcagcgtgcc acggtgaatc tggtcaaaaa accgaagcac acgatcctgg
    13081 gagtgctggc ttatcaaagc attgccgatg cctgtgatga tgttccgcgc ttgctggaat
    13141 tcaaacccag cgccattgag ttgattccgc agatgatcat tcaactggct cgcggtgtcc
    13201 cttcatttgc ccgccaaatg ggttggatga cgggaaaccc tgccgcggtg ctggtcattg
    13261 aatttagcgg ggatcaaccg tcggctttga aagaggcggt tcgtcgtgtc ggcaatgtca
    13321 ttacgatcgc cgagtcgccc gaggaacagg ctcgtgtatg gaacatccgc aagatgggat
    13381 tgggaatttt ggattcgcgc ccgcaagccg caaggcctgc cgcattcatc gaagattgcg
    13441 cgatccccgt ggaacgattg ggcgaatttg tgcgtgagat cgagcggatt ctgcgcgacc
    13501 atcacaccga aggtggaatt tacgcgcacg cttcgggcgg ttgtttgcac attcggccga
    13561 ttctaaatct gcatcttggc gagggagtgc gggctttgcg aagcattggc ggacaggttt
    13621 tgcaattggc gctgcgactt ggcggctcga tgagcagtga acatggcgat gggatcgcaa
    13681 caggcgagtg gatcgaaaaa acatacggtg cagaagtgac caaagccatg cgcaccttga
    13741 aacacgccgc cgaccccaga aatattttga acccgaagaa aatgttcgat gcgccgccca
    13801 tggatacaaa tttacgatat ggcgaaaatt atcaggcgaa gacctgggtt ccatctttgc
    13861 atctcgatca tgagcgcggg ctggcaggtg caatcgagca ctgcaacggt cagggcgtat
    13921 gcagaaaaac caacggggtg atgtgtccct cttttcaagc gactcgcgac gaagccaatt
    13981 caacacgcgg cagggcaaat cttcttcgcg gattaattac cgattaccac ttaccttccg
    14041 cagaaaacgc aaggcagaaa gaactcatgc aggccaccta ccaagccctt gatctttgcc
    14101 ttgcctgcaa aggctgcacc tccgaatgcc ccagcggcgt ggatatgccg aagttgaaat
    14161 acgagttcat gaacgagtat tacaaaaccc atcgcagaca cctgcgtgat tatttgttcg
    14221 gatatttcca tgtggtttcg aaatggcttg cgcctgcggc accgctggca aatgcgttca
    14281 tgcaaatgag ctgggctcag aaactgatcg cccgcacaac ggggatcaca gagaagaggc
    14341 cgttcccatt atttgcaaag agaagcagcg cacagaaagc ataccccaat gcatggaaaa
    14401 atggaaaagc cgtcatcttt ttatcggatg tgttctcgcg ctatcttgaa cctgaagtgg
    14461 aacaagctgc ctttgaactt ttgcatgcag cagggtacga ggtgaaaaca ctgcccatca
    14521 tcggctcggg ggcatctctg ctttcaaagg gattcgttga tgcggcgcga aagcatgccg
    14581 aaaaggttct cagtgaaatt catgcgcttg atcccgattc aacgttgagc gtggtcggat
    14641 gcgaaccgcc agaagtgtat tgcctcaagc atgaatattc ggctttgctg ccaaatcgcc
    14701 gcacggaact cgaatccatt tccaggcggg tgtggctggt ggatgagttt ttgttacggg
    14761 atgaaaacct cgaaagattg gtgcagatgg gggaaactgc cccccacagt gaggtcatcc
    14821 attttcatcc gcactgccac caacgcgctg aagggctttc agaagacggc ctgcccaatg
    14881 gaaccgccgc caccacggaa ttgctgcgcc gcttcggctt tcaagtgaat ctgctggata
    14941 caggctgctg cggcatggcg ggtaccttcg gctacgacgc aaaccactat gagctttcaa
    15001 tgcaggtcgg tgaattaaag ctgtttccaa agatcaggga attgggattg ggaaatggag
    15061 acttggcttc ttctggctcg gcttgcagat tgcagatcaa gcagggagtt gggaagaccg
    15121 cggagcatcc gttggtggtg attgcgaagc gcatctctcc aaacggataa agaaaatccc
    15181 gccccgtaga aatctacagg gcgggatttt tgtattatca attttttcgt ctactcttgc
    15241 gagatcgtga cgctttccat cgcatcacca ataaagctgg gattttgctg gggatcgcga
    15301 agcgtgagtg aattgacaac atccatgccg ctgacaacct gaccaaaaat ggtgtagccg
    15361 ccgttcagcc attcagcagg gccaaaggtg atgaaaaact ggctgccatt tgtgtcagga
    15421 ccagcgttgg ccatcgccaa aatgccaggc ttgtcaaagg tcaggtcgct atattcgttg
    15481 acaaactcat aaccaggtcc gcccatgccc gagcctgtgg ggtcaccgcc ctgcgccatg
    15541 aatccttcca aaacacggtg aaaggtaagg ccgttgaaaa atcctttgca ggtgaggaac
    15601 acaaaactgt tcacagtgat cggggcttta tcagcaaaca actcgatctt gaattcgccg
    15661 ccgttcgcca ttttgaaggt ggcgaaatat ttggccgtgg tatcgatcaa aatttcaggt
    15721 gcggaattgt attgggctgc cgcagggatc gaatcgaaca ctgcgcaggc agaggaagat
    15781 ccgcctgcag gtggaacaac cgcaggagcc gttgcttcaa cgacggctgg ttcagtggca
    15841 agcggtgcag aggtcgctgg agcagacgga gagagggaac aggcgagcga ggcgatcacc
    15901 aaaacagcga ccgcaaattg gatcttgata tttttgtaca tgaaaacact ccttgagaat
    15961 tgaattacag ggggctattt tatgaagata atgaaaatcc cgccacccca aatgccacag
    16021 ccacattgaa cgagcgtttc tgtccgtgca tggggatgtg aaaaaccgca tcagcaagct
    16081 caagcagttc ggggtcaacc ccagtcactt cactgccaag cagcatcaca acgggggatg
    16141 ggaattgaaa ctcagcataa cgcgaaatgg gaatcgagcg ctcgtcttct tccagagcaa
    16201 caactttcca accttccaac tttagacctg taaccaactt gaccgaatct ttgtgatggc
    16261 tccaggtcac aaattcatct gcgccgaggg cggtcttgcg aacggcatcc acttcgggcg
    16321 tgggggtgat tccacacagg taggcatggc tgaacccaaa tccatccgcg ctgcgcagga
    16381 tcgaaccaac attccatgcc gagcggatgt tatccaacaa cacagctttc atttccttcg
    16441 gtttacttct ttcttcatcc acaacagcct ctttttggat ctgtctcccc aacacgacgc
    16501 gagtctctcc caggcaatgc gggcagcgca ccccaaagga atgtccaatt gtgattgggt
    16561 aacgcaatcc gcaactttgg cacactcgaa cttcaaaaga tacatatgac atgccccgat
    16621 tatattcgct tgtacaccaa accccagcct gataattttt gactggcaat tcagacaaca
    16681 agcggtaaaa ttaaaagatg cagacaacta gacagctggt gccagaattt atctatgata
    16741 aatttcgcaa taacgagata agcggggtct ttcaaggtgc cgccatgttt gttgaccttt
    16801 caggcttttc aaccatgaca gacgcccttt cagcacacgg tacatatggc gccgaagttc
    16861 tggcgaatat gatgcgtgtg gtcttcgagc cgttggtgaa tgccgtgtac gaacagggcg
    16921 gatttgtgat cggatatgcc ggggatgcgt tcaatgctgt ttttcccacc gagcaggatg
    16981 caggtcagac catgaggcgt tgtctggcag cggctgtcca aatgcaggct cacgccaaag
    17041 ccaatcaact tgcatccaca ccttatggag atttctccat ccgcatcaag gtggggattg
    17101 ggtatggaca ggtgacctgg cagatcttca agtccatcaa tggaaaacgg gccagttatt
    17161 ggttccgtgg tgaaagcctt acgggggctg ttcttggtga agaacgcgca tacgcaggcg
    17221 agatcgtagc cgacccgatc tcttacaaac tcatcagcga tattgtggaa gttcgacccg
    17281 tgcaggaatg ttttctcatc gaaaaggtga ttgccgacct gcctgcggcg cttcctgttc
    17341 caacgcccga gcctgccgac gacctcgcaa gcattttcct cgccgacacc attgcccacc
    17401 tgcccatttc tggcgagttt cgtcaggcca tgaacctttt cattgatatt cctgtcaaca
    17461 tttcagatca ggctttgatc acgcctttta tggaaacggt ttatcttttg caggaacaat
    17521 acggcggcgt tttcctgcgc cccgatcttg gcgacaaagg tttcaacctg ctcatgttct
    17581 ggggcgcgcc catcgcctac gaaaacgatg tggaccgggc gttgaatttc atcatcgaac
    17641 tggccacccg aactcgcatc acattgcggg ctggcatcac gtatcacatg gcttatgcag
    17701 ggtttatcgg ttcaagcaaa cgcgaagatt acaccgccta tggatggggc atcaatctgg
    17761 ctgcccgcat gatggaacat gcaggtcagg gcgagtattg gatgtctgaa gaagtggccc
    17821 gccgtgctga aaaacatttc aacctcaact tcctggggga acatcatttc aaaggtttca
    17881 agcaggatca aaaggtcttt agcctgctcg gaagaaaaaa cattgttgaa accgtgtatc
    17941 agggccaact ggttgggcgc gaaaaagaaa tggaaaattt gcgctctttt acagaaccca
    18001 tccatcgtgg aaactttgca ggtgtgatgg ttttgcaagg cgaggcaggc atgggcaaga
    18061 gccgcctcat ccattctttt cagttttcag aatattttac tgaacacccc gcccgttggg
    18121 tcgtttgcca ggccgatgag attctgcgcc tgccattcaa tccattcaaa gactggttga
    18181 aaaagcgttt tgaattactg gaaggccagc ccgacgaaat caacgcaatt gccttcaacc
    18241 gcaacctgca ggaacttgcc aacatcaccc cagaccccga actggccgcg gaactcatcc
    18301 gtacccgttc tgtgctttcc gccctcatta acatcaccca gcccgactcc ctgtatgaaa
    18361 gccttgatgc aaaaggccgc tacgacaaca ccctgatcgc cttaagtgct ttgttgcgcg
    18421 cagaaagtct gcaaaggcca ctggtcctct tcctcgaaga tacacattgg atggatgaag
    18481 atacaggcaa tttcctttcc tacttcatgc gcaccctgct ggcagaatct gaaaaacaat
    18541 accccatcgc catcatcacc acccaacgcc ctgaaggcaa tgcaaattgg gaattggatg
    18601 gaacttacgc tcaccacatc aaattagaga aacttccagc cgccagcatt gcccatctgg
    18661 cgaaaaacat cgtagaccat ccgatctcag catccctgct tgagcttttg aattcccgca
    18721 ccgatggcaa tccttttttt gccgagcagg ttttgcgcta tctcacggaa cataacgcat
    18781 tgagcctgaa tgaagaaggg gaatatattg ccaatcaagc cgagaatatc tccctgccaa
    18841 ccgatgtacg cgccgtgctg gtggcacgcc tcgaccgttt gaccaagcag gtgaaagagg
    18901 cagtacaaac cgcttcagtg cttggccgcg agttcgaaat ccgcgtgttg ggtttgatgc
    18961 tgaaaaatga taaaaggctt ctatctcatg tggcacaagc cgaaagcgcc aacatctgga
    19021 ccccgctcag cgaaatcgaa ttcatcttcc gccatgccct attgcgggac gccgcctact
    19081 ccatgcattt ggtcgcacgt caacgcgaat tgcatgggct ggcagtttct gccatggaaa
    19141 cgatctatca cgaggatctt gagccacact acggagaatt agcctatcac gcagaaaaag
    19201 cggccctgaa cgagaaagcg cttcactacc ttaatctggc agcggattca gcgtcacagg
    19261 catatcaaaa cgcccaggtt attgattatc tcacccgcgc ccataaccta ctgccgcaaa
    19321 atagccaaca tgaacgtttt gaagttctgt acaaacgctc cattgcatat tccattatcg
    19381 gcaatcgaag cagcgaggat atggacctca ccgcccttga agaaacagct tctgcgctta
    19441 gagacaacac caaactggcc agagtattaa tgcgacgcac ctattatttt gccacgcaag
    19501 gtgaaaactt catggcgatc tcatttggtg agcgcgctgc cacactggcc caatcagtca
    19561 atgatatgga gacattggga gaggtttaca acttcctgcc cagtgcattt acacgcgtgg
    19621 gaagtttgaa agaagccttt gaatgcgctc agaatgccct gaagctttca caatccacaa
    19681 acaaccgcct tggggaaggc aatgccctga cttcgctggg attaattgca ctcgaaaata
    19741 agggaccctc cgaggcttat caatatcaag ccaacgcctt gaagatcgcc cgcgaattgg
    19801 ggaatcgtga cctggaagca aaagtactta ataatctggc taatttaatc ggcttgtcta
    19861 acggtgacta ttccaccgcc cgtgactact tcgagcaggc gcaggtaatt taccaggaac
    19921 agggaagaat gtatccttcc gggcttgcgc tgaccaacct tggctgggtt tccaacatgc
    19981 ttggagatta tgaaacagcc ctaacctacc atacgcgtgc ccttaccatt gcacgtgaag
    20041 taggaaataa aacccaggaa atgtacacac tcatcaacct gagcgcatcc acatttggca
    20101 agggaaacaa tcccaacgct gcaatctatt gggctgaaaa agcgctgtgg ctttcagaaa
    20161 aaatcgccga ccaaataggc accgcatggt cattttttta tcttggctat gggtacatgc
    20221 tgggcaaaca gtaccctgcc gcccgtcagg catttgaaag ctcgctaaaa atcagggaag
    20281 gacttgacgc aaaggtacta acagccgaat cccaagctgc gattgggcaa actctgtacg
    20341 aattaatgga cattgaagcc gccttcatcg aggttgagaa agttttggaa tatatgcaag
    20401 aaaatccaaa ccttgaaggc gcagaggaac ccctacgtgt aatgctcaca tgctatctcg
    20461 tcttaaaaga aaaaagagac ccacgccata aaaacatttt gcaaaacgca aatctgcttt
    20521 tggatacgca ggtctcaaaa ttacgttcag atgaggcacg tcacgtctac gtggaaaatg
    20581 tcccctggcg acgcatgctc aggaaatatt acaaggaaac gcagtaactc ttaaggccaa
    20641 tgaccgatcg cgggatcgcc gtctgttaat tcaaaacggt tgttcttgat ctcacctgta
    20701 tagtagtagg gatttccatt cccgtcaaaa atgatcaact ttgaagagcc aatattcgga
    20761 atggtgacaa aaaatctctg attgatgtac tgtccattct ttttgatgga gaagttgttg
    20821 atccaggtaa tggggttacc gtccaccatg ttggcaggca ggccttcgac actgagcttc
    20881 tcaacatcgt aatcgccatc atccacgata tcaatcgtga tgtctgattc agagacttta
    20941 tccttctcga tcttgtattt tttattatta tccttatcct tcttcacctt gcctggcaca
    21001 acgactttgt tttgcatggg gactctcctt gggaatggga tgatatgtca aaatccacct
    21061 ttggggtggc gtatttcaaa gacgggcata ttatatcgcc tgtcattact aaccaataaa
    21121 ccaaataagc gttctatgga tttttgccgc cagatctcaa aatcatggct gcgccggagg
    21181 ctccatagag agtcataagc gccatccaga tccaggtctc tgcagttgcc caattgacct
    21241 tcttttcagg cggcaccact gcgtccacta gaagcaggcc aatgattgca caggcaccaa
    21301 ttaccagcag agtcagaccg agggttttcc attccagttt ggaagcctga cgggtaatca
    21361 gaaatatccc aacggcgggc gtaatgaaaa caacgctgta catctgtccg tgaaatgcat
    21421 caatgggcca gggccaaaac gcagatactg cggttggggc gatcaacagt gcaagtccgt
    21481 atagcccgaa caaggctgac aaccccaagg cataattccg ccacgtcgca ggggttgggg
    21541 tggattctgc tggcgcccac gctcgataaa gccacaaatg ataggcactg tttagaggca
    21601 aaacaacata caggagaaac cagatccacg gctgccatct ctgcagatca aactggtcaa
    21661 gatggaacag cgaaacgccc agaacaatcg ttgtgaaaat aaaaatcatc ggaaggacga
    21721 tccgtccggg cgaccatcgc ccataaaatg ccatgaaccc cgctgaaaca aaggaaccaa
    21781 gatacactgt cccgacaaaa cggccattga acggagcaat gacccagggc cactgctccc
    21841 gtgcaagggc tggcaaaaag aaaagtccgc cgcccgttac gatcaggacg aggacttcca
    21901 accaggtaat gctgcgcaac aaagacgaaa taggtgggtt tgcagaatcc aactgtgaat
    21961 ttctcatcat atgctccaat caataattta ggttctacaa cagcggcagg gtcaaccgca
    22021 caattaattg ggccgctgtt atccgcttct gggattcatc ttatgcaagg aatccaagta
    22081 caggtcttcc actttttttc tggcccaggg ggtctttcgc agaaacttca aacttgattt
    22141 tatgctgggg tcattggtaa agcacttgat ctcgatacgc ctgcccaatt ctgcccagcc
    22201 ataatattcc gacaattgag tgacgatcat ttcaagggtg attccatcag ggatgttttt
    22261 agggcggtct gaattcattc tacagtcctt ttactctgct gcaggcagag gttcaagatt
    22321 aatgagctgc gagacagtta ccagggtata tcccttgctc tgcagataat caatcaccaa
    22381 aggcagggct tcagccgtat gccagcccct cccatttcca tgcataatga tgatcgaacc
    22441 gttttgcact gtccgttgaa cctcagccag gatcgttgcc gcatcgaagg tcggatcagg
    22501 gtctccggtg accgagtccc attgcactgc atacaaccca tgttcagaaa caatctgcaa
    22561 ggcagtatcg ttataaaacc ctgatggaaa gcgaaacagg tgcgaacgtt tccccgtcaa
    22621 ttgaaacatc atatcctcgg tcatgtcaat ttcctgcccc atttgttccg cggtcaaggt
    22681 tgtaaaatcg gcatgactcc atgaatggtt acccaattca aaattcgggt tggcccccaa
    22741 caaccgcgtt tcgtcaggat gggtacgcat ccaatcaccg cccataaaaa aagtggcagg
    22801 gacgttatac cgctggagaa catccacaat ccctgcatca taccctgccg ggtataaggg
    22861 atcctggcac atatcgaagg ttaaagccac gtatgggaga gtccagtcac cgctggtgac
    22921 gagagcagca ggcggagttg gggttaggta tgcagttgcc gttatcgtag ggaccgggct
    22981 gggcgtgaaa gaagctgcga ctgtaggtgt gacatcagga gtggacgtta caagcgattg
    23041 aggcgcggcg caggaaacta caaacaatcc gatcacgcaa aatacaatgg ctttttttcc
    23101 catttccact actcccaagt ccacttgttg tgaccacgcc cagagaaaac ttctaaggcg
    23161 ctgggctgga catttcgaag taatcaaaca aagcagggat ggtggtgctg atgccatcca
    23221 cattctggcc tgccaaaaca cccacttgga ggggagtcat atcactttca tgtgccccga
    23281 ctttataaaa caccttgcca tcaccgctga tctcaaaaat ataactggtc gccttgcggg
    23341 tcaagcgcaa gactaccgca ttgttcttat aagattgggc aaaattgggc ggcacgatca
    23401 atccccgtcc gtagtaattc atgtagagcc cccggctcac acacccgccg ccgcgacaat
    23461 atccctgccc ggcctgaatg aagttgttgt tatcttgata aacgaccagc ccggcaaatt
    23521 gaaaattctg atcaggttca aaggtcagga tggtttcgat ctggaaatcc cccgccgggg
    23581 caggcctgag gagaatattc gtcatggttt cagcgttgac cgatccgcgc ccgccagcga
    23641 tgcgcaacga tcctggagcc ttgtccaaac tccattgtcc gggaatctcc cgcagccagg
    23701 tccacccagg ttgaagaatg tcatcgaaat catctctaaa atagacaggg tcaacggtcg
    23761 gcactatggg tgtctctgtt gtttgaagca ctggagcagc agttggggta tttgtggaaa
    23821 cgtccggagg ggtaatctcc aacggagttg atgttgttgt ggcattacca ctgcacgcac
    23881 ctgtcacaag caacacagca atgaagacaa aaattccgat aaggcttttc gtggttttca
    23941 tcctgtaaat tataaagccg attgtatgga aaccccaacc caccttgttt ttacgtgttc
    24001 ataaaaaaac agcggatacc atttcggcat ccgctgtttt ggaagaggct caaatcatcc
    24061 cttgaagaag tttgaaataa agaaccagat attggcagga cgttccgcaa ggcggcgcat
    24121 gaaatacggg taccaatgcg tcccaaatgg aacatacacg cggaccgggt atccttcttt
    24181 cacgagtctt tcctgcaagt cacggcgaat gccatacagc atctgaaatt caaggccatc
    24241 tttgggaagg ccgactttat cagcgtattg ttttgcaaag gcaatgcgtt tctcgtcatg
    24301 tgaagcgatc gctgggatgg cagggaaacg gccatctgct gaaagtttcg tttgaaaggc
    24361 aagggaggca tcgatcagta ttttggtcaa gagatcgtaa ttcgcatcca catctgcttt
    24421 tcttggaaaa gccttctcag gcggttcttt ataagcacct ttcacaagac ggatggtgat
    24481 ctgatcctgt gtcaggcggc gggtatcggc ttcagcgcga taaagatagg actgaaccgc
    24541 catgcccaca ttggtataac ccttctcacg catcatgtaa tataggttga tggttttatc
    24601 agtatagggc gtatcttcaa tatccacgcg gatgaatgtg ttgttctgcc gtgcccgggc
    24661 caggattctc tcaaggtttt cagcacacaa gttttcgtcg aggcccagac cgatctgtgt
    24721 cagcttgatg gatacgttac cgcgcagcgt cgcttcggcg ccaatggcat ccagtgtggc
    24781 aaaaatatca tctgtggctt gttgtgcttc ttctggtgta ttcgtatgtt cccccaaatg
    24841 atcgagcgtg gcgttaatcc cctttgcatt taattccttg acaacccgta cggcgtcgct
    24901 cagattggtg ccagcaacaa aacgggatgc ggcacgccag gcaaaactcc agttcgtaat
    24961 caggttttgt gcccaggctg ctttggagag gtagatgaag aaagaacgga gcatttcggt
    25021 cctttttgcg agaaatatag agcagttaat gtattttagc acaagcgcat ggtttggtgt
    25081 gttatacttt tttccgtgat atttgtcatt tcttggaggt tctgtgagaa atcgtaatac
    25141 caacacaatc cttcggggtg tgtcgattct atttttgact gccgcgctcg tactcagtat
    25201 catctccctt attacctaca gccgccaaag gaataattac ccagccagca tgaccattgc
    25261 cggcgtaccc gtcggcgggt tgaccccagt tgaggcatcc cagcgcctgc tcgaagtgta
    25321 cacctcaccc gtggaattgc tctacaacga cgccatcatc cacattgagc ctgccgtggt
    25381 gggtttcgag cccgatattg aaaccatgct ggccgctgcc gaccttagcc gaacgggcgg
    25441 ttcattttgg ggcggctact gggatttcct ctggaaccgt accccatctg aaattgccat
    25501 tccactcagc ccgaaatttt cagaagaacg cctgcgagaa tatctccaaa atgagattgc
    25561 cgcacgttac gatgtccccc cctccccggc tgtccccgtt cctggcggga ccgatttcct
    25621 gcccggccaa cccgggcaga ctcttgacct tgagcgcgcc gtcctgctcg tcaacgatgc
    25681 acttcgctcg ccgaccaacc gctctgtagc cctcaccttc acccgcgggt cggtcgcacg
    25741 tcccaccatc gacaacctga ccatcctgct caaacagatc gtgcaggtct ccgatttcga
    25801 cggtttgatc ggcctgtata tgatggacct gcaaaccgga caggaaatcc acttcgccat
    25861 caacaacaag gaagaaatca gcgtcacacc ggatatcgcc ttcactgcct ccagtacgat
    25921 gaaaatccca gtggtggctt cctatttgat caaccgtggc agcaatcttt cgcccgatat
    25981 tgccaacagc atttcacaaa cgctcggcaa atcaagcaat gacgccactg atgttgtcct
    26041 ctctgccatc gaacccaacc gtggacccgt gatcgtgaca gagaacatgc gcagtatcgg
    26101 cctcgaaagc accttcctgg ctgggttctt cacctccccg gcctacctgc ttccagccat
    26161 tcccatcacc ccggccaact cacgcactga tgtcaccact gagccagatt catattcaca
    26221 gaccacccct tctgaaatgg gctccctctt ggccgatatc taccaatgcg cccaaagcaa
    26281 tggcggtgca ctaatcgccg cattccccga caaggtcagc ccgcaaacct gtcaactgct
    26341 gatcgacttc atggcccagg ataaactcgg ttcgctcatt caaggcggcg ttccagatgg
    26401 aactctcgtc ccacacaaac acggctacgt tcccgccgca gacggtgtgg tccacgatac
    26461 cagcgatgtg ggcatcgtat acacacccgg tggaaacttt gtcctctctg tctacaccta
    26521 ccatcctgtc accaacattt gggatatcac caaccccttg atcggggata tcacgaaagc
    26581 catctacaat tatttcaacg taacaagcca gtaaaaataa ccttacagtt caataactct
    26641 ccgacccgtc ttccaacgaa aacgggtctt tttttcgcac atcgtatata attcgaccca
    26701 tgagcgcatc gttgggattg ttccgtctac agcaagtgga tcgtaaaatt gaccgtgcgc
    26761 gttcgcaatt ggagaccatc cgcaaaacac ttgaaaacga caccgaattg cgagaatgcc
    26821 tcacccatgc tgaaaccgcc aaagcttctc accatcatgc gcatcacaac ctcaaaagcg
    26881 cagaaatgga agttgaaact caaaaaataa aaatagagca ggccgattca agtttatatg
    26941 gcggcagggt gcaaaacccc aaggaattgc aagaccttca aaaagagatc gtttcgctaa
    27001 agaaacacct tgccaccctt gaagaacgcg aattggaagc catgcttgcc gcagagaccg
    27061 ccgagcaaaa cctgcaagca gccagcacac aactcgagtt gatccaggcc cgccttggga
    27121 gtgaacacac aaaactgctc gcggaccaaa acgcactggt agccgaactt gaacaactga
    27181 ttgaagaacg cgaagcaacc ttatcgccgc tggacacttc cctgctccaa atctatgaag
    27241 acttgcgcca gcaaaaacgc ggaattgcag taacagagat cacagacaat tcctgtgctg
    27301 cctgtggcgg cacgctcaac tccgccattc agcaaagcgc ccgttcacaa aaactcgcaa
    27361 actgtcccac ctgcggacgg attttatatt caaattaaac gtctcccatg tctcgatttg
    27421 cgattgaccc ctactccttc ttcgtcggct ttattgcagc atccattttt tggctgctgg
    27481 tatcccgagc gcgtccgcta tgggccgagt tggttgcggg tatcaaagaa caacgtgaaa
    27541 tcgcacaaac ccgcaaatcc agcaccgtgg aagaaaatca tcgccgcatc accctgcgcc
    27601 gcgcacaagg gatgcatctc gccgcaccgc ttttctcgtt ggatgagatc atcctcgaac
    27661 ctttgctgat agccccgcct caagttgtac aacccggtac accagccaaa tttgaagatg
    27721 tagtttcaca aaccctgccc tacatgccca tgtggccaga gatcgccgct gcatatcatg
    27781 cgcaaaccct gaccctgaca caagccttgt ctggcaacgc caacatcgtc atcatgggcc
    27841 agcccggcgt cggcaaaact gtagcacttg cacaccttgc atccctcgct gcaaatcgaa
    27901 gcgcaaagct ggaaacgctc caaaacgccg ttccatttct ctatcacgtt gccaatctaa
    27961 aactcccgct gcccgacccc aaaaaagacg tccttgctcc gctcattgaa gcagcatctg
    28021 aaaacgcccc gctcatggac ctaaaccgcc tgccgggctt tttcgaaggc gccttcaaaa
    28081 gcggtgaagc ccttttattg atcgatgggt ttgacgaaat cacagccgaa taccaacagc
    28141 ttgtcaccga ctatttcaaa gccattatgc aggcctaccc aaagacacgc attgtcacta
    28201 cgggtgcgcc cgaatatctt gatggattaa ttccgcttgg ctttgcccca ttggcattaa
    28261 tgacctggtc tcaacgggaa aatcagaaat tcatcgaaca ctggggggaa ctttggtcgc
    28321 aaaccgtggc gatggaagcc tgggcacaga ctggtcctga acaggtggac ccgctcttac
    28381 ttaacacctg gcttggtact gacaatttga atttatcgcc ccttgagttg accctcaaag
    28441 cctggggcgc ctacgcaggc gatagcctcg gcccgcacgt gctggaagcc attgccacac
    28501 acatccgccg cattgcacct gccaacacac cacttgccgc tcttgaaacc ctggcgatgc
    28561 aagcgatggt caacacccaa cccatttttg atccacgggt ggcccgggca tgggtgaagt
    28621 cttttgaagt tgcagaagag actcccaccg gcgaagcgac tgaacaagcc gcggctggag
    28681 aagagaccga agaaaaagca gaagacacca aacaactcaa aggcaaaaaa gtcgaaaaag
    28741 tctccacacc cacttcaggt ctgcttggca aaatggcagc cagcggattg ttggtggttt
    28801 atccgaataa caaaatgcgc ttcacccatt cagtctttgc aggctttttg gctggccgcg
    28861 cattaatgag cttcaaagcc gatgaaacca tcctcaatca agcggattgg tctggcaagt
    28921 atctggtgat gcgatatttc gccgcacatg gtgacgccag taaactggtg caatctctca
    28981 tggaattttc acgcctgccc atgcaccgtc cgctgtttgc agcggcgcgt tggctgcgtg
    29041 atgccccgcg caatgccccc tggcgcggaa aattattcgg cacactggcg gccattatgc
    29101 aaagtgaagg cctgcccctt aatctgcgtg gacaagccat ggcggctttc gtcgtcagca
    29161 acgattcaag cattacaacc ctcttccgtc agcttgcggg cactctctct tttgaacttg
    29221 ttcatctggc cgtgctaggt gcaggtgcca tgcgtgatgc gaaagccctg gaagttttgg
    29281 agcgtgcatt gcaggcacca agcacagccg tacgcagggc ggcctgcatg gctttggtct
    29341 cggtgggtac caatgatgcg ttggagactg ttgctcacac cctgctcagc ggagaggaag
    29401 atatccgccg cgccgccgca gaagcactgg ccaatgatcc gcacgaaggt tacgccatgc
    29461 tgcgtgatgg ggtcaccatt gacgatattc tcctgcgccg cgctgccgct tatggcctga
    29521 gccgtgtcag tgaagaatgg gccattgaac tgctgaaaaa gatccagatc gacgacaagg
    29581 aatgggttgt tcgcaacgcg gccaccgaag ttctggactc aaagaccagt gcaggctcac
    29641 ttgcaccgca caaacttaag gcaccatccg aatctccctg gctgatcgaa tttgccgcca
    29701 aacagggaat gggtatttcc cccggagtcc ctgccaccga tattctttta ctggctctca
    29761 agagcgatga tgcagaaacc cgccttgcgg ctctgcctta tctcaaatac acccccaacg
    29821 aaggcgtgat ctcacagctt tatgccgcaa tgtacaagga tgaccatgaa ttgcgcgagg
    29881 ctgcctacaa tactttatgg gaattgggag cctccggtgt gaagctgcca gaccccagcc
    29941 aatatggatt tggataaaca tgctcataac aaacgcaaaa atcatcacgt gggaaaccac
    30001 caatcaagtc cttgagaacc atgcaattct gatcgaaggg gatcgtatca aagagatcgg
    30061 caccaccaag gcgttgacgg caaaatatcc aaaagccaag accctcgatg cccgtgggca
    30121 gtatgtgatg ccgggcagca tctgcgcgca cacacatttt tacggcgcct ttgcacgcgg
    30181 catggccatc cccgggcctg cacccaaaga cttccctgag attctgcaga agttgtggtg
    30241 gcctcttgac cgttcgttgg atgcggaagc agtccgcttt tcggcgctgg tttgtttggt
    30301 ggatgcgatc aaacacggca caacaactct gatcgaccat cacgcctcac ccaacgccat
    30361 tgacggctca ctcgatatca tcgccgatgc tgtggataag agcggattgc gcggcgtgct
    30421 gtgctacgaa gtaaccgacc gtgatggcag tgagaaagcc aatgctggca tcaacgaaaa
    30481 tatccgtttt ttgaaacgac tggccagcaa cccgcatccg cgccttgctg gaaccttcgg
    30541 cctgcatgcc agcctgacgc tttcgggcat caccctgcaa gcctgccgcg ccgccgttcc
    30601 cgaagggacg ggattccatg tccacactgc agaacatgag tcagatgaat atgacagcct
    30661 cggcaaaagc cagatgcgtg tgatcgaccg cctgcaaaaa catgggattc ttggtccgaa
    30721 caccatcaca gcacatggca tccactttga tgcacgtgaa atggaaattc tcaaagagac
    30781 tggcacctgg ctcacccacc agccgcgttc gaacatgaac aatggtgtgg gcgtggctcc
    30841 cgttgaatcg atgctgcgac tgggcgtcaa agtttgtttg gggaacgatg gtttctccaa
    30901 cgccatgtgg gaggaatgga aagccgcata cctcctgcac aagatccacc accgcgatcc
    30961 acgccgcatg ggcggatatg atgtgacgca aatggcggtt tataacaatg ccgccctggc
    31021 aaatcaattc ttccccgccg ccccgatcgg gcagatcaca caaggtgcgt tcgccgactt
    31081 gatctttgtg gactatcatc cctacactcc gctgaccgct ggcaacctgc catggcagat
    31141 catctttgga tttcagcaaa gcatggtcac cactactatt gcagcaggca aagtgttgat
    31201 gaaagaccgt gagttactca cattggatga ggcggagatc gccgcccgtg cccgcgaaat
    31261 tgctccgcgc atctggaagc gctatgaaga agaagtagca aaagtaatgt agccagaacg
    31321 aaatcgtatt tccatacttg gagacttaat gaacgactca aatcttttat tatccatcgc
    31381 cgtattgggc ggcacaggca aagaaggcaa gggactcgct ttccgttggg cacgcgcagg
    31441 gtatcacgtt cacattggat cgcggtctgc cgaaaaagcg attgccgcag cgaatgagat
    31501 cagggaaatg ctcggggatg ggatctccat ctcagggatg agcaacccgg aggcggcgcg
    31561 cgattcgaat atcatcgtgt taaccgtccc atattccgca catcgcgata ctttggaatc
    31621 cgtcaaggaa gaactcaaag gcaagttgct ggtggacgta acggtaccgc tggtaccgcc
    31681 caaagttgcc accgttcaaa tgcccgctgc tggctctgct gcacaggaag caaaacagat
    31741 cgtcggcgaa gaagtgcagg tctgtgccgc gtttcaaaat atctcgcacg aacacctact
    31801 ggataactcc gaggttgagt gtgatgtgtt ggtcacaggc acaagcaagg aagcacgtgc
    31861 tgaaaccatc aaactggttg aggcggctgg cttaatcggc tgggacgcgg ggccgcttga
    31921 aaactctgtt gttgtcgaag gacttaccag cgtgttgatc ggcatcaaca aaaaatacgg
    31981 ttccacccat gctggcatca agatcacagg cgcgaagcgc aacacgtaat tcgtaactca
    32041 aaattcggcg tttcgaattt tgggccgcgc gttacagaat tttatgtctc tttccatcac
    32101 cccattacaa aatattcccc ttatccgcca aggcgacaac ttggcggata ttcttgtcgc
    32161 ttcaattcag aattcgacta ttcaacttga aaacgacgac atcctggtcc ttgcacaaaa
    32221 aatcgtcagc aaatcagaag gccgcatggt gaaccttgcc acggtcacac catcgtcaca
    32281 ggcgctcgaa ctggctcaaa aaactgaaaa ggatccgcgc gttgtacaac tcatgctgca
    32341 ggaaagcaac gagatcgtac ggacacgcat cggcgcgatc attgtggagc acaaacttgg
    32401 ttttgtgtgt gccaatgcag gcattgacca ttccaacgtg gccggggacg gcagcgagaa
    32461 tgaagagtat gtcctcctgc tcccggaaga ccccgatgca tcagccaaag ccctgcgcga
    32521 ccagatcagg caaaaaacag gcctcaacgt cggtgtgatg atcatcgact cacatggccg
    32581 cgcatggaga aacggcacgg tcggcatctg catcggctta agcggaatcc ctgccatgat
    32641 cgatgaacgc gggtggaaag acctgttcgg ctacaccttg aaaatcaccg tcgttggcgt
    32701 ggccgatgaa ctcgccgctg ccgcatctct aatgatgggg caggcctcag agggaatccc
    32761 cgctgtgcat gtgcgcggtt ttccataccc cctcggtgaa ggctcactca aggagttgat
    32821 ccgccccaaa gaacaggata tgttccgcta aaaaactaac acaaatctta tccgcagttg
    32881 atacactcgg tttgagaatc aacatcatga aaaattttcc accagaatat ctcgacctgc
    32941 tcaaagacga aaccagggct tacctttttc ttgcaaccac catgtccgac ggctcgccgc
    33001 aagtgacacc agtctggttc aacatggatg acgaatacat cctcatcaac accaacgaag
    33061 gccgcaccaa agaccgcaac atgaaagccc gtcccaaagt ggcatttgtc attcaggacc
    33121 ccgaaactcc ctaccgctat attcaggtcc gcggcgaagt tgccgagttc acaacacaag
    33181 gcgcagatga acacatcaat cagctatcgc tcaaatacga tgacaggccc tggaaatatc
    33241 gtgaaggaca aaagcggatc atcttcaaaa tcagaccaac tcactttgac cgccactgat
    33301 ctccacacct ttctgcggac tcgacgctcg atccgccgct tcaaaacgga ccctgtgcct
    33361 gatacggtga tcgaaagcat tctctccacc gcgacatttt ccccgtctgc gcacaaccgc
    33421 cagccctggc gttttgccgt cgtaacagca tcagccgtta aagaaaaact cgccgacgcc
    33481 atggccgttg aatttcaacg cgatctcgag gcggatgcga tctccccaga aaatgttcaa
    33541 agaaaagtca gtcgctcgcg tgaaaggatc gttgccgccc ctgtggtcat catcctcagc
    33601 gtagacatga ccgatatgga tacctacccc gatacccgtc gcaaaaaagc tgaatacatc
    33661 atggccaccc aatccaccgc gaatgcgggc atgcaaatgc tccttgccgc ccacgccgaa
    33721 gggttgggga gtgtctgggt gtgcagcccg ctgtttgcac aggaaactgt tcaaaatgtt
    33781 ttggggctgc ccaggagctg ggagccgcag gcaatgtatt tcctgggtta cccatctgaa
    33841 acccctgaag tacgtgaaag aaaatccatc aaggaaattg taaaacgtgt tgacgttcaa
    33901 aagtcaacac cgtgaagctc aatccatgaa aatcactgcc ctcgcgggcg gagtcggtgg
    33961 cgcgaaactg gcgcatggac tcgcccaaat cctcccttct caagacctga ctatcatcgt
    34021 caatactggt gacgatttcg aacatttggg gctatccatc tgccccgatc tggacacagt
    34081 ctgctatacc ctggctgggt tggcaaaccc cgaaactggc tggggacgtg tcaacgaaac
    34141 ctggaacacc atcgcaaata tcgaaaaact gggcggaccg aactggttcc gcctgggcga
    34201 tcaggatatc gctactcacc ttgaacgaac cagaaggcta aaaggaggtg aaaccctttc
    34261 tcagatcaca aaagattttt gcaaagcgtg gggaattcag ccaacgattc tgccaatgac
    34321 cgacgcaccc gtacgcacga tcgtggattc agatgaaggc gagttggcct ttcaggaata
    34381 ttttgtccac aggcaatgcg ggccgaaggt caaaggtttt cggtttgacg gagtcgaagc
    34441 cgctgaacca gctttcggcg tgcgcgaagc ggtcgaatca gccgatgcga tcatcatttg
    34501 cccgtcgaat ccatgggtga gtgtcgaccc aattttgcgg gtcattccgc ttccaaaaaa
    34561 gaaaatcgta gcggtttccc cgatcattgg tggaaaaacc gtgaaaggcc ctgcggcaaa
    34621 gatgtacgcc gaattgggca ttgagccttc cgcgctggcg gtggcggaac attatcgaga
    34681 ccttttaagt ggttttgtgt tggataacgt ggatgcacat ttatcggata aaatcaggac
    34741 aaggactttg gtaacagata ctcttatgaa ttcacttaca gatcgcgccc gattggcgat
    34801 ggatgtgcta cactttatcg ggagtttata aacatgacac tttgggcaat tgtccctgta
    34861 aaacctttac gccgggggaa atcccgcctg gcgggcatgc tgacagaaga tgaacgaact
    34921 gctttaaatc aggaactgct ggagcggact ctaaaaacgc tctcaaccct gaaagaattg
    34981 gatcaagtgc tggtggtcag ccgcgaccct gccgcactga ccattgcgcg caaccatggc
    35041 gccaagaccg tgcaggaaga tggacagccg cacctcaata cagctttgat gcgtgcaacc
    35101 gttgtggcac aggtccatgc cacgcagggg gttttgattc tgccagccga cctgcccctg
    35161 ctcacccctg atgatgtttt gaccttgatc gaccgcgcag ccaaaccgcc tgtggttgtg
    35221 atcgcgccag acaggcatgg caagggcaca aacgccctgt tgatggtccc tgccggccaa
    35281 attgaatacg actttggcga gggttcattc cagcgccact gcgaacgcac caaacagtcg
    35341 ggagcacggc tggagatcgt agaactcccc tcgctcggcc tcgaccttga cctgcccgaa
    35401 gaccttgaaa tggtacgaaa aatggaagct ttaaagtgat cggaaattaa ttggcccaga
    35461 aggtcaatga cacccaaatc tatttttctc aaggagaaaa acccatgacc gaacgtgttg
    35521 ccctgtacct gcaagattcg cacgacctgc gcgatggttt ggattacgcc aaatacgcag
    35581 aagaaaaagg attcgaagcc gtttggcagg cggagagtcg tttggtgcgc gatgccattg
    35641 tgccgatggc gggttacgct gctgtaacca acaagatcaa agtcggctcg ggtgtcatca
    35701 acaactggac ccgcaacatt ggcctgctgg ccgccacttt tctgacactg gatgaccttg
    35761 ctccaaatcg tgtcatctgt ggattgggcg catggtggga cccgctagcc aaaaacgtag
    35821 gcattgaccg ccgcaagccg ctgaccgcga tgaaagaaac ggtcatcgtg atgaagaagc
    35881 tgctcaacat ggaacgcgtt tccttcgatg gtgagttcat tcatgtcaat ggaattgaat
    35941 tggatgtggt ctacggccgc cgcgaaccgc gcaatgtccc catcatgatc ggcgccacgg
    36001 gcgaccagat gatggaactg acaggcgagg tggcagacgg tgctgtgctc aactattgtg
    36061 tagcccccga atataacgac cgcgccatgg aactccttga aaaaggcctg agaaaatcgg
    36121 accgcaagat ggaaaacttc gaccgcccgc agctcatcgt ctgctcggtg gatgaagacc
    36181 acgacaaagc catcaactat tccaagatgt tgctctgcca gtacatcgcc cagcagcctc
    36241 acattgccaa ggcgtctaat gtctccgacg atgttgttca tcagatccag tccattttag
    36301 gctggcctgc caccaaggaa caggtgatga aagccaaaga cctcgtcccc gatgaactcg
    36361 tccacaagat cacagcttca ggtacaccct ccgaagcccg cgccaaagtc gctgaataca
    36421 cgaagcgcgg atgcacctgc cccattctct accctgtcgg cggcaatttc aaactcctca
    36481 tcgacacctt cgcacaaagc tagcccgcag gcaaatgtca taagcaacac atgagccacg
    36541 tcacttgacg tggctttttg attcaactat cctacccgcc gatagactga ccagtcggtc
    36601 tagggatcat gaaaccaaat tcacccaccc tctccccgca ggaaacccgt gaacgcatcc
    36661 ttgaagcggc cgttacagtc tttgccagca aaggctatca cgacaccaag gtggatgaca
    36721 ttgtcagcga atccaacacc tccaaaggct cgttctactt tcacttccca agcaaacagg
    36781 atattttcct cgcgctcgtg gatacctttg ccgacctgct ggaaacgcga ctcaaagaac
    36841 gcctctctgc ttcgacctct ggcctggaac gtgttgaact ggcattacgc gtctgtttgg
    36901 agaccttcgg ccaataccgc gccctggcaa agattgccct tgttcaggcc actggccttg
    36961 gtgctgcttt tgaagaaaaa aggcgcactg taaacgaacg gttcatccaa tttatcaagg
    37021 aaaacctaga cgcagccgta gcggatggaa gcatccccgc gttggatacc gaagtgaccg
    37081 ccagcgcgtg gatcggcgcc ctgaacgaag ttgtcatgcg ttggatctac acaggccagc
    37141 ccgaccctga gcggtcgctg ccagccttga gaaaaatatt tttgttaagt cttgaaatcc
    37201 caatagagag aatcaatcaa taagatgtaa cgcggacttc agtccgcaat tcacaaaaaa
    37261 aagcggacta aagtccgcgt tacaaaacac cttaggagaa aaagaatgag aagaatcaga
    37321 tccttgttgg cattcgtcat cattgcatta ttggctgcgg cctgcgcgcc cgctaccaca
    37381 gcaccgcagg aaacagtgac cttgaagatc gccgtcctgc ccattatcga cacactgccg
    37441 atgtacgttg cgcagcagga aggtctattc gccaaacatg gcgtcaatgt agagttcgtg
    37501 cccgtggcct ctgctccgga gcgtgatcaa cttgttgggg ctggtcaggc cgacggtatg
    37561 gtcaacgaaa cgatttcggt catgttgttc aacaaggaaa aagtgcaagt acaagcggtc
    37621 cgctacgcat tgcgccccac cgaaaatgca ggccatttct tcatcatcgc ctccgccaag
    37681 agcgggatca ccgatgcgca aggcttgaag ggaattgaga tcggtgtttc gcaaggcaca
    37741 atcattgaat atgtcaccga aagactcttg caagctgaag ggttgacgag cgacgagatc
    37801 aaaaccatcg ccgttccaaa gattcccgac cgaatggccc tgctcgcatc aggtgaattg
    37861 aacgcaggcg tgctcccaga cccgctaggc gcactggcag tgcagcaagg cgcagtcatt
    37921 gtccttgacg actccaggca ccccgaatat ggtttcagcg ttatctcctt ccgcaaagaa
    37981 gtcattgatg caaatcccgc tgccatcaag gcgtttcttg cagccatcga agaagcgaca
    38041 accatgctca acagcgactc aaaaaaatac accagcgttt tgagcgacca gaaacttgtc
    38101 ccaccgccgc tgcttgccag ttacgcagtt ccgcccttcc ccaccgcagg tgtgcccacc
    38161 gaagcggaat ggagcgacgc actggcatgg gtgaaggaaa agggaatgct gaccacagat
    38221 gtttcctatg cagattcggt caacgcaacg tttcttcctt aaccgataac tgaacccgtt
    38281 ataatgaatt gacagtcacc cgtggaggtg actgtcaatt tttgaagcag acatgatcac
    38341 ggtttcttca ctcactttca attacgccaa caacaccccc attttccagg atttcaactg
    38401 gcaggttcaa cgcggggaga cttgggccat cctcggctct tcgggctgcg gaaaaacaac
    38461 cttgctttat ctattggctg gcctgcgttt tccctccaaa ggccaaattg aaattgacgg
    38521 agaacgcctc acccgtccgc gcccgcacag tggattgatc ctgcaggatt atggtttact
    38581 tccatgggcg accgtgcagg agaatgcagg tcttggatta aaggtccgcg agttttacgg
    38641 agaagatggg aaacacgcgc caaaaaactt tcagcctgaa aacaatgtgg cgcactggct
    38701 cacgcggctg ggattaagcg aagtcgccga caaataccca tctcaaatct ctggtgggca
    38761 gcgtcaacgc actgcgatcg cgcgcacact ggcactttcg ccagacctgc tattgatgga
    38821 cgagccattt tcatcacttg atgccatcac ccgcgaagac ctgcaaaact tgaccctctc
    38881 tctgtgtgaa gaacaaaaat tgactctggt catcgtcacg cattccatcg aagaggctgc
    38941 atcactgggt gggaaaatcc tcctgcttgg gtcatcacca cgcgtttttg agaatccaac
    39001 cgtgcggcag ggcgatcacc gtaacagtca ggaataccgc gatctgtgca atttgttatg
    39061 gaaggaaatg cgcaatgaaa cggcgtgacc ttcttttctc tgcggcaggc cttgtactca
    39121 tctggcaggt tgccgccatg ttgatccagc gtcccatttt gcctgcaccg tgggaagtgc
    39181 tggtcgtttt cgtgcaggaa ctgggtaacg ggttggtcac tcactttttc tacagcctgt
    39241 ggcgtgtcac ggctgggatg gcgctgtcgg tgctgatcgc tgcccctgtc gggctggcag
    39301 ttggcgggtc gaaacgactc gatgcaattt tttccccgat catttatttg ctgtatccga
    39361 ttcccaaggt ggtctttgtc cccgtcgtgt tgttattcct cggcatcggt gacgtggcca
    39421 agatcaccat catctttttg atcctgttct ttcagatcgt ggtgctcgtg cgcgatcaag
    39481 cggctgggct tgcgccgcaa ttgatccaaa gcctgcaaag tctcggggcg ggccgcaggg
    39541 cgctgttccg ttttgtttat ttgcccgcca gcctgcccgc gatcctgacc gcgctgcggc
    39601 aatccattgg cacagcggtg gcggtgttgt acatcacaga attgtttgca accaaatatg
    39661 ggcttgggta ttacatctac tacaacggca gcacgctgtt tcattaccct gccatgtacg
    39721 caggtgtgat cgcgatgagt ctgctcggcc ttggaatgta ctacacagtt gattggctgg
    39781 aaagaaaatg gtgtaaatgg aagtttgtga gttgaaggtt
//
LOCUS       DAOQLC010000023        39029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_298_length_39029_cov_22.666419, whole genome shotgun sequence.
ACCESSION   DAOQLC010000023 DAOQLC010000000
VERSION     DAOQLC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_39029_cov_22.666419"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..793)
                     /locus_tag="PL000_06020"
     CDS             complement(17..793)
                     /locus_tag="PL000_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HNC88486.1"
                     /translation="MSTQNFMPHDGVNQILSALLEKAKTILGDQFVGMYLYGSLSSGD
                     FHPDASDIDFLIVTCEALPEEAISKLETMHNELWKSGMEWASKLEGSYIPQKPLRRYE
                     ANGEAYPSINESKFYVAPHGSDWIIQRHIIREYGKALEGPDPKTLIDPVSPDEIRKAV
                     TGILKEWWFPMLKDPSWLREHDSHYHSYAILTMCRALHAFEHGTIVSKPVAAKWTQEK
                     LGGKWVQVIQSALDQRIDAGHFELIDDAMDLIKITKELTS"
     gene            complement(843..2459)
                     /locus_tag="PL000_06025"
     CDS             complement(843..2459)
                     /locus_tag="PL000_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNC88487.1"
                     /translation="MLSIHNLSKSYGIQPVLQNINFNISAGERIGLIGPNGSGKTTLM
                     RILAKLEQPDAGRGDVSSTRPNLRIGYLAQGMDLDPRQTLHTALGLDPATQTDPAAEI
                     ESLALALSQNPNDSALQTQYDHALTKLSALNLQPETILAPLGLDHLPADTPIAHLSGG
                     QKTRLMLARVLLEEPQLLLLDEPTNHLDIEMLEWLEEWLNQFQGAALIVSHDRVFLDN
                     TVTSILELNPITHGIRMYAGTYSDYMEAKQKEQDKQWDEYRDQETEIRRMKDDINRTK
                     QQALHVELTTTPRQPGVRRIAKKVAVKAKSREKKLDRYLRSDERVDKPLQGWHMKLEL
                     GEPEHQSKIVLETDSLSIGYALESPLLENINLFIRAGQRIVLTGPNGAGKTSFIRTIV
                     GRIPPLAGSFRLGGATKLGYMAQEQELLNPKFSALQTIQSVSAFNETDARNFLHYFLF
                     KGDAALRASEELSYGERARLQLATLVAQGCTFLVLDEPINHLDISSRERFEEALENFK
                     GTILAVVHDRSFIDRFASDVWVAKDGKISK"
     gene            2713..3558
                     /locus_tag="PL000_06030"
     CDS             2713..3558
                     /locus_tag="PL000_06030"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC88488.1"
                     /translation="MTKTLLAVLAHPDDESFGLGGTLALYASRGYDVYYVCATRGEVG
                     TVDAEHLKGFKDTAEMRTDELMRAGKELGLKEIFFLGYRDSGMPGTEDNKHPDAQINA
                     PIDEVAGRVVKHIRTLKPEVVLTFDPIGGYKHPDHIHIHKATVLAFEKADDASFHPEA
                     GAAFKPKALYFQVFPKGLLKAAVRLMPLFGKDPTKFGRNGDINIKELAEVTFPIHVRL
                     NVASAAEAKQKASACHASQGGGQMRRGLMGFVTRMFGENEDYMQAYPPVAGSSKRKTD
                     LFEGI"
     gene            3639..4049
                     /locus_tag="PL000_06035"
     CDS             3639..4049
                     /locus_tag="PL000_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88489.1"
                     /translation="MKHLRLLFVSLVLVALLAGTVTTPSLAYPDSTTTIVKVPVKPNR
                     SLPNADTYDCTKATLYNRNGSYAATFTNPPASNGCTFIFNNVPVNQWYTVEFLMKYKL
                     GSPPYIVIRTSQSVYIAKPTLTTITTATLLYRPK"
     gene            4186..4932
                     /locus_tag="PL000_06040"
     CDS             4186..4932
                     /locus_tag="PL000_06040"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNC88490.1"
                     /translation="MTESNPPLPTRRFNFARILEVLLHPKRAFAEIAGETVSSWLTPM
                     LALSISSSLVVIVSGFLKARAAAMGEIQLPPDWQYWTPDMQNNFMQAQQATQGATFVY
                     IIPLLSALAGLWLGWLILSGLLHLGSTLMGGRGTMGSALNVVGWGGLPFLLRDVLRVV
                     FMLIVGHTIVSPGLSGFAGGAVFLSKMLSRVDIFVIWNAILLILGFGTADGLTRNKAI
                     LVVIVILVVILLALAGAGTLVSSFSSGAAF"
     gene            4947..5684
                     /locus_tag="PL000_06045"
     CDS             4947..5684
                     /locus_tag="PL000_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88491.1"
                     /translation="MTSPLIQIRDLKKHYQMGGTTVRALDGVSLDIEPHTFTVVMGPS
                     GSGKSSLLYLLGGLDRSTSGEITISGERLDQMDENTLALFRRRTMGFIFQSYNLIPSM
                     TALENVSFPMQFSGIPVSERNSRSRQLLEQVGLGERSHHRPTELSGGQQQRVAVARAL
                     VNDPSLVLADEPTGNLDTASGVTVMQLLSDLHRSGRTVLVVTHDPRMTRFATHKIFLL
                     DGHVVTEEEYQSASMDMMQTLSDSPSA"
     gene            5705..7420
                     /locus_tag="PL000_06050"
     CDS             5705..7420
                     /locus_tag="PL000_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNC88492.1"
                     /translation="MKFQSKYFVSILLAAAFLASWATPVYADPIISSIDPNLVFNDAP
                     STVTITGSGFESGAVVSLKNYGDLTTSFDGPNQLRAVVRPGLPAGSYTVVVTNPDATT
                     FEYPNGLTVADTSPLPTPTSAPFDRPQMVIGSYEVNVQRVRSGDDFKLNIDFGNAGTV
                     AAYNVQAVFTSADLIATKTGGVIFLGTIRAGDHNDATQPFIALDSIYGKSVVTTDVTL
                     TYYDSAGTEYTDKFTLSIPVGGSNVPYPTATPTGVNTAQLVITSYGSTVDPLQPGEQF
                     QLGLTVQNMGNTSAKNVTMIVGGGSSGDSSGTPQPGGVSGSGGEFTNFAPVNSSNIQS
                     LGDLPAGGQLQVFQSLIVNVSTNPGAYPMKISFSYINNKGEVVNDDQVITLLVYSLPN
                     VDVSFYRPPDPFFAGQPGALPIQIVSLGKRASVLGNMKIETTNGMVDPATTLVGSLDP
                     GGYFTFDTTLIPDVAGPMELTVTIEYTDDFNQPRTITRKLQVTVEEAFVDPALDPSLD
                     PSLGGGGGEFIPMEEETLLQKIWRGILGLLGLDSASPANNFPTEGPALEGEPLPMPAN
                     GGKGG"
     gene            7427..8764
                     /locus_tag="PL000_06055"
     CDS             7427..8764
                     /locus_tag="PL000_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88493.1"
                     /translation="MKFLDLLRLIFGNLGRRKARVALTAIGVVIGTAAVVILVSLAVG
                     LQKSATDQLYGIGDLTLIDVMPAYGVFYGGGYGAPMMGGGGGGGGGLDEPALITNSVL
                     EQFRAIPGVHTVIPRDYLNASTIISYRKLEAGMGFIGIDTDDLANIGLEASEGTTILS
                     RGSVVIGAMTPNNFYDPNLRPGQEPPLPPNLYNEQIQMVVMKWDQEGNVSQKIISLRV
                     AGVLTETGGEADYSIYLPLDLVRSLNEWANGVRINYNKTGYNQVIVKVDDPNQALDIA
                     DQITAMGYQAITPQAYLESINGYFLVLQIIFGGVGTIALLVAAIGIANTMAMSILERT
                     REIGLMKAVGATNRDVLSIFLGEAAGIGFIGGAGGVIIGWLAAQGINVLAIIYLAGQA
                     SEQGGPPPSVAVYTPVWLPVFALIFATIIGMLSGLYPALRAATMIPVQALKYE"
     gene            8945..9286
                     /locus_tag="PL000_06060"
     CDS             8945..9286
                     /locus_tag="PL000_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC88494.1"
                     /translation="MPERRTTPRKKFDYYLRVVDDDTQGILGHMVEVSPIGLQLETTA
                     PCPVEKDYYLRVELTPELADRPFIIFIARSKWCRIDEIEPNLYHVGFAMAEIMPDDRE
                     IFLRILARFGR"
     gene            9409..9774
                     /locus_tag="PL000_06065"
     CDS             9409..9774
                     /locus_tag="PL000_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC88495.1"
                     /translation="MSKRNIVHVEIPAANVEGAGKFYQELFGWKLQHMPEMNYTMWEA
                     GDGSGGGFPAVSAENPAGQVLVYIDSDDIEADLKKAEKLGGKVIFQKTEIPGMGWFAV
                     FHDPTGNAIGLYTSLNPAN"
     gene            9791..10087
                     /locus_tag="PL000_06070"
     CDS             9791..10087
                     /locus_tag="PL000_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88496.1"
                     /translation="MATGDFAFVTSLEPAQKDVFLNREKYDIVYASILEILRMHGPMT
                     FTQLGHRVEERLQDGFDGSVLWYFTIVKLDMEACGEIQRVVNSKPQLFSLNVNV"
     gene            complement(10161..11471)
                     /locus_tag="PL000_06075"
     CDS             complement(10161..11471)
                     /locus_tag="PL000_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88497.1"
                     /translation="MFKNPNHRNIFGIWLGWALIMLAYHNLLPARLTVQPPDRALEWT
                     VTETMPGSQYGKIYLNEPFLNRHVSWDSEYYLAIAVGGYEDPSPTRIRSNTGETTESG
                     GFWPFVVPPQSAERPGISLSYAFFPFYPMMIRLFALPLSLLGMNAIATASLAGVIVSM
                     LGTLAAMIALYEFARTELDESSGLRAAFYLLIFPSGFFLAEIYTEGLFVGLAFSSLLL
                     LRRGHRGWAALVAVLATFTRAVGVTLAIPLFISWIKDAEWLELDMEWKQIYYRGLPWR
                     ILVNGLIATAPIIAFFLWRISYYGLAFSLVEEEFFGRGLLSLGYTFIAWKDGFMEIFG
                     NNPNAAAYYFVEWLGIIIGFAACIAGFKRHPDLAIFGFLVVFLSFTSGPAQGMYRYVL
                     AAPPVFLFLSRLGKNPIFDRIWTIASMLLMSMMCIMYMFDMWAG"
     gene            complement(11476..12552)
                     /locus_tag="PL000_06080"
     CDS             complement(11476..12552)
                     /locus_tag="PL000_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC88498.1"
                     /translation="MKTKSRHKWLEPHNYLSLQLWETLGSALFNLFIFFMLFVYLFPM
                     LFMVATSLMEGIQLRDRNAPAYPSQQVRFEYQGEEYLVYNVPINGGVRQLALVEPKQT
                     SSKFIDPQNPEDGLIPWKGSWRQLKGVYRFHVTFDNFKILFSSLRIPEMVKNTFMMTI
                     ISEIGVLLSSIVVAYGFSRFPLPGGNFLFYVLIATILIPEKVTLIPTYFFFARVLKWQ
                     GTWLPILIPFFFGNAVYIFLLRQNFKSIPKDLDEAAMLDGAGPLRILFQVILPQSWPT
                     VLTVSLLHFYYMWNETRQAALYLSTRRDLAPISFGVQSFQSLAPIQNQLQAGALILMI
                     VPVIVLIISQRYFMRDMVVTGMEK"
     gene            complement(12555..13460)
                     /locus_tag="PL000_06085"
     CDS             complement(12555..13460)
                     /locus_tag="PL000_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC88499.1"
                     /translation="MSTGNTKRLRYDRRFGLLLIAPWVIGLLFFKLLPILASLGISFT
                     NFQLLTPEQTQFVGLENYARLFQDEAVGYVLFETIALGISTIPLQLAASIVLASMLAN
                     PRLKFGNTMRTLFFLPSIIPGVAIFFMWIGFADPTTGWLNKLILHPLGLTGFNNLYSD
                     GAVALLFSISSLWSVGPGMLIMLGALQGLPPEIQEAARVDGAGPLVRFFYITLPLISP
                     AIFFSIIINMIAVFGGVVLLDRGSVFSGSFSPYDGYVSYILFDKMDLGYAASLAWFFF
                     ILVMIVVVALFSSARNWVYYPDREA"
     gene            13531..14727
                     /locus_tag="PL000_06090"
     CDS             13531..14727
                     /locus_tag="PL000_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88500.1"
                     /translation="MKPEQKSRFNDLFFARLYYFCFMGGWGFVLPFINLFYVSIGFNG
                     KQIGVISSVSAIVGMLVSPLWVSEVKKRPQARNILQAALILGGIGYYAIGLQSQFLWV
                     VVIIFLHALAASGIAPLSDSMAVTVSQEAGSGYGSVRVWGSLGWIFTVLSSGWLTARF
                     GFIAAFIGVALMWLTAASLVFLIQPRFFSAQPASAQVRPNLSAALQYIWHDKVLRGFA
                     LALIIFGIFNNGVVQFENVFLAELGATKQVISVAGIMSALVEIPFMLFSDRIVQRVGA
                     HRLLMVALTMTIFMRLSVLTFPAILTIMAVRFIGGVAFSFYTISFIKLVSSRTQPTET
                     GTVLAILSVTITGLVNIIASPISGAIFDVIGARWLYALSATGYVISIACLWWTRPEKQ
                     PAGSAG"
     gene            complement(14724..16085)
                     /locus_tag="PL000_06095"
     CDS             complement(14724..16085)
                     /locus_tag="PL000_06095"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNC88501.1"
                     /translation="MANKITFPENFIWGAATASYQIEGAWNKHGKGESTWDRFTHTPG
                     KIRNNDTGDVANDHYRLWKKDIALMKKLGLKAYRFSVSWPRVLPTGRGKVNQKGLDFY
                     SKLVDGLLNAGITPFVTLEHWDLPQALEDKGGWTARATTEAFVEYADVMSRALGDRVH
                     QWITHNEPAVVAWLGYETGVHAPGIKDLSASLRAAHHLLLSHGMSVPVIRNNSKDAEV
                     GITLNIGWKVPASNSKMDMDAARLDDGKWFRWFADPLYGRGYPSDMTKYFTQLGGAPN
                     GLDFVQSGDLETIAVPTDFIGLNYYGRNLHRVEDPNNGPQLFFPHPKTPEHWTEMDWE
                     NYPEGLTGILGRVYFDYTPLKIYITENGASYSTAPDENGNVPDVHRTNYLRTHFAAAH
                     RAMQAGVPLGGYFVWSLMDNFEWSFGYAQRFGIVWVNYETQERILKDSAKWYKTVIRK
                     NGF"
     gene            complement(16089..16952)
                     /locus_tag="PL000_06100"
     CDS             complement(16089..16952)
                     /locus_tag="PL000_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88502.1"
                     /translation="MAESHLPIASIENDFLRLDFFTTTGPRIIGLYARGANGNLLAET
                     PDIHWPTPHGEFFLRGGHRLMTAPEDLFFMGPEDGLQMERSHNTVILKSPVDASSLER
                     EITIRLDENRAHLSHRITWHGKDEIRFAPWAITQMRMGGMAIVPQSNADGLQPNRNLV
                     LWPYTRIHDPRLELNDDLILLHGKVSAEACKVGTHTPLGWLACAMGDALFIKSFEVTA
                     GDFPDQGCNVEVYVKDTCIELETLGPLKTLKHGHSVTHSETWQVLTGSYPATLETART
                     ILRDHSNQDGA"
     gene            complement(16955..19426)
                     /locus_tag="PL000_06105"
     CDS             complement(16955..19426)
                     /locus_tag="PL000_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HNC88503.1"
                     /translation="MKFGYFDDKAREYVITQPDTPLPWINYLGSEAYFGLISNTAGGY
                     SFYKDARLRRLTRYRYNNSPLDMGGRYLYLRDNGTGKFWSPSWMPTRTKLDEYECRHG
                     QGYTIITSKFNGIKSSTRYFVPLGESLEIWQMTLTNESGSDSDLSLFSAIEFCLWDAN
                     DDATNFQRNYSIGQMEIVDEVIYHKTEYRERRDHFAYFACSEKLAGFDTIREDFLGHY
                     RSWNEPQVVESGRSSNTEAHGWQPIGSHQVKVNLKAGESKQIVFILGYVENPKDEKFD
                     PPQSQVINKKRAKIALEKYLKPENADQAFNTLREYWTGLLGKLQVNTPDEHTNRMVNI
                     WNAYQCMVTFNMSRSASYFESGIGRGMGFRDSNQDLLGFVHMIPERARERILDLSATQ
                     LKNGGAFHQYQPLTKRGNNDVGSGFNDDPLWLVLGVSAYIKESGDWSILDEMVQYENE
                     AGTETPLYEHLQRSIQYTLDRLGPNQLPLIGRADWNDCLNLNCFSDTPGQSFQTTTNK
                     EGKVAESVFIAGLFVLACKEMADLAGKMKEERGDSFVFHLSSDDYLKFATDMESTIWK
                     SGWDGDWFRRAYDDFGHVLGSKENSEGQIFIEPQGICIMAGLGLENGNAAKALDSVAQ
                     HLATPHGIVLQQPAFSQYYLHLGEISSYPPGYKENAGIFCHTNPWIMIAEALVGRGDA
                     AYDYYKRINPSAREEISEMHKCEPYVYAQMIAGKDAATHGEAKNSWLTGTAAWNYAAI
                     TQHILGIRPTLDGLEISPAIPASWDGFTATRQYRGVTYNITVENPRHVCKGVETVTVN
                     GRQVSGGLIRAENGETEVDVRVVMG"
     gene            complement(19450..20250)
                     /locus_tag="PL000_06110"
     CDS             complement(19450..20250)
                     /locus_tag="PL000_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNC88504.1"
                     /translation="MKRLIINSDDYGRTPEVSRGIREAHLHGVVTSTTCMMNIPTTAA
                     DIQVALREAPLLGLGVHLVLTADRPIGPHESVRSITDEAGNFLNLSVLTQRIHSIEVS
                     EVKAEWRRQIEAFIQAAGKKPTHLDSHHHSSYFSAALFRAMLELAKEFDCAIRLPFME
                     GNDAIKEYAPALLAEFNPRRANTFYASFYDELATRAELQRIIASIQDGTCEIMVHPGY
                     ADEALFKASSYARQRETELEILTDPAIRQAIADRGIELIHFGSVEQDT"
     gene            complement(20279..21511)
                     /locus_tag="PL000_06115"
     CDS             complement(20279..21511)
                     /locus_tag="PL000_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HNC88505.1"
                     /translation="MSQPFTLGVNYWPRRKAMYWWSNFDAGEVREEFSLIKEIGLNVV
                     RLFLLWDDFQPDPASVAKDKIENLVKVADIAAEHGLGLDITFFTGHMSGPNWSPRWLL
                     GGDLPPKAHNWIRDIVSEGKKTDKGYRNMFHDEMALNATRLLLKTVVTTLKDHPAVWM
                     WNLGNEPDLFAWPNSSEEGAAWVKEMVGLIKSIDPNHPVTIGLHGDGLHRDNGLRIDK
                     VYAHTDVAVMHSYPMYTPWARQPLDPDFVPFTCALTAALAGKPVLMEEFGGCTALPGE
                     TTYTMKWTETNGREREQFMASEEDFAEFLRLTIPKLQHSGATGAMLWCYADYVPELWD
                     MPPCQNSQHERFFGLVRPDGSLKPHARVIQEFAKTNPQVLPIPDYAKLTVDADEFYKE
                     PLPHLVDLYQKYLTDLSK"
     gene            complement(21541..22401)
                     /locus_tag="PL000_06120"
     CDS             complement(21541..22401)
                     /locus_tag="PL000_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HNC88506.1"
                     /translation="MTQKPSFRIVAYATDAITPSAIPYHQLTHINYSFLIPKADGSFV
                     NINNAWALEEIVAAAHENNVRVNIAVGGWGWDNEFEAMAAVPEARSAFVSNLAAFVEQ
                     YQLDGVDIDWEYPDQGQSAQNFLALMQELRAALPEKEISTAVISFGDDIGLGVPNEAF
                     ALVDFVNVMTYDGEIHGSLVEFKKGLEYWSARGVPKEKINIGVPFYSRPSEVTFAKLV
                     QFDPAAAQADAFEYNGSLENYNGIPTVQAKTELALQNAGGIMFWALDHDAQGQYSLVN
                     TIYQTIHTNP"
     gene            complement(22479..23444)
                     /locus_tag="PL000_06125"
     CDS             complement(22479..23444)
                     /locus_tag="PL000_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNC88507.1"
                     /translation="MTYFLGIDIGATKTAALITNANGEPLGAGQGGPGNHETVGFDGM
                     EKAIREALNDALNTANIRMDAIAGAGFGIGGYDWPAEETAMRSAITNVGITAPFKMVN
                     DVTLGLLAGTSEGWGVAVVSGTGCNCRGWDRDHKREGRVSGYGTLMGEGAGASELIYR
                     TMQIIGYSWTKRLPPTLLSDAFIKHFNAKNLEDLIEGYTNGYYPIGADAAPLVFQTAE
                     SGDETAKKLVHWAGIELGELANAVIRQLEFESLEFEVALIGSMFKSGEALITPMRETV
                     HALAPGAKLIPAEVKPVIGAVLLGMEASGLKLTNSERTKIKAVLA"
     gene            complement(23459..24757)
                     /locus_tag="PL000_06130"
     CDS             complement(23459..24757)
                     /locus_tag="PL000_06130"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HNC88508.1"
                     /translation="MLKVAVIGGGSTYTPELINGFLARLEKFPVNELWLMDIDKERLD
                     IVGGFAQRMVEAKGSPFKVVLTTDQRAAVKDADYVTTQLRVGHMEARKRDEYLGLRHG
                     LIGQETTGVGGMGKALRTIPVILKIAKDMQELSKPGAMLVNFTNPAGLVTQALSMYAK
                     DTPSVGVCNVPITAKMSILEGLEQATGRKIEPERAELKTLGLNHLSWHRGFTVDGEDV
                     WPQVIQGYIDELKKEEHPEWEPRTVEVLRMIPNYYLQYFYHTDKKLKAQEAWPPSRAE
                     EVIEVEKDLLRQYADPALNEPPADLMKRGGAYYSTVATQLLNAHYNDLGETHVVNIKN
                     NGAVKEWPAEWVLEMPSTVKKSGITPIAAEPLPTSCFGLISAVKAYELLTVEAAVHGN
                     RDAAYQALLVHPIGPKADKVQAVLDDMLETHREHLPQFFK"
     gene            complement(24902..25948)
                     /locus_tag="PL000_06135"
     CDS             complement(24902..25948)
                     /locus_tag="PL000_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC88509.1"
                     /translation="MHSKPLSPLQKRISIFTVTAFTFAILFIYLSPFAFMVLTSLKTQ
                     DQISIVGAPFYPAAPPKFEYNGDKLDVFVVPFPDGTTRELAMLKKGQKESTFIDPQNP
                     TGSEIVWQGSWRTLDRPWTFSPTWENYAEVWNNIDFPRLLWNTTFYAIMTEIGVLFSC
                     TLVAFGFSRFRFPGRDFLFVILIATIFLPAFVTVIPTYTFFAKIGWVGTWLPLIVPSF
                     FANAYDVFLLRQYFMTLPRELDEAAMIDGASPLRVLWSVIIPQSYPALMAVTVFHIVF
                     AWNDYFGPLIYLSTNRDNWPISVALSTFNGIYGQKPQLIQAGALMALILPLILFAVAQ
                     RFFVQGIVVTGVEK"
     gene            complement(25961..26986)
                     /locus_tag="PL000_06140"
     CDS             complement(25961..26986)
                     /locus_tag="PL000_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC88510.1"
                     /translation="MFEELANFRKYIPSIKSFRNASPLARRELKQGMAFLSPWLIGFF
                     AFTFLPIIASLIFSFLDLNITDGILNTPKFVGLANYIQLFNDRQAGVDPRTWITWLAG
                     GTPSSMGVTISFGLIALPVGILVPLGLAVLMNNPNLKGQMLFRSLFYMPFIVPFVASV
                     FLWGGMLNSETGWINRALIGLGVPKEVTPDWANSTTWVYPTYVIMGIWGVGNAMLIML
                     AGLQGVPTELYDAAKVDGANGWQTFWKVTFPMISPVIFYNLTLNIVGLFQYFLVPLVV
                     NQGTGRPGGATMFYSLYLFKNFFAFQDMSYGSTLAWFLFIVILLITFILFGTAKYWVY
                     YAGDSRS"
     gene            complement(27058..28449)
                     /locus_tag="PL000_06145"
     CDS             complement(27058..28449)
                     /locus_tag="PL000_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC88511.1"
                     /translation="MKKHLLALFSLMIVASMVLTACGGSAPAEKVKIRWFVGLGTGTD
                     PAQVTVQEEVVKDFNASQSDIELVLQIVPFDSARDTLSTQIASGEGPDIVGPVGWGGS
                     NAFYGQWLDIAPYIEETKFDTSVFDPALLKFYQTEEGQVGLPFAVFPGAMYYVPSMFD
                     EAGLAYPPQKYGEKYTLDGKEVDWNWETVTEVAKRLTIDVNGVNATEEGFDRTQIVQV
                     GYSAQWQHPNSVATFYSGAAKIYSGEKKGEYASALPDGWKEAWHWYYDGMYGDQPFIP
                     TGPLANSPEFGTGNVFNSGKAAMGLTQTWYTCCLGDFAKAGNEFQLGIQPMGSDGVVH
                     GRIDADTFRIWKGTKHPKEAFQVLAYLITTGGDKLLPAYGAMPAIASKTQAFFDKKAA
                     DYPFVTKESWDVFNQGLAYPDTPSAEQYQPNWKEAFDRQQVFMDLMNNTEGLDFDAEF
                     QKLVDDLNVIYNK"
     gene            complement(28541..29575)
                     /locus_tag="PL000_06150"
     CDS             complement(28541..29575)
                     /locus_tag="PL000_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC88512.1"
                     /translation="MTRKSDSVTIRQVAEKAGVSVATVSRYINHNAPISREVSDRLKK
                     VMAELRYVPHAAARHLASKKTRVIGLLLTNMHNDFFAPLLSGIESVVREHKYNLIVAT
                     NRADEETVVSPPVGSHNTDGLLVFADSLSDKVLIDLYNSEYPVVLIHRSSPPSVNIPS
                     VTVENKAATFHLMEHLIHEHKKRRILLMRGPIHQEDSYWRELGYKSALEANGIPFDDS
                     LVLQGDFERDVAYRSLNQFLGNGKRVAFDSVFAGDDDAAVGVIASLKEHGFRVPEDIS
                     VAGFDDVKISAYLTPPLTTVSAPTESVGRIAAEQLFSIFDKQLPEKVTLLPTELIIRH
                     SCGCSHEVNP"
     gene            complement(29778..30995)
                     /locus_tag="PL000_06155"
     CDS             complement(29778..30995)
                     /locus_tag="PL000_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88513.1"
                     /translation="MKRNLVILWAVWFTLLYGFQWLVTTRLQLKQPDEAVVWSANETL
                     PSSNDGKIYLLEPFMNRQVAWDSEYYVGIAVGGYDDPEAGYVRNPATGLPIAKNYSFF
                     PLYPYLMSAVAFPLRIFGLSAIGTATLAGSIITLLGTLAGLFAVWDLTQGLFDEDSAW
                     RAVFYMLVFPSAFFFAQIYTEGLFVGLAFSALMLTKRKQWVWASILGFLAAWTRAHGA
                     AMALPLLLAWIINTDWKSNLRSQLTWKWFLQGLLAFLPLIGYFIWRTSPLGKGWEELQ
                     AFYFGRGLMTIESSLQSWKDAYFYARYVSQEGLIYFGIEVFTILLGLICSIWLIRRDP
                     IVALFSLAVVVLSVFSGSAQSMARYMLIAPSIFITLGYWGKNKVVDKAWTLISILLMG
                     MSVLLFSYDMWVG"
     gene            complement(31005..32348)
                     /locus_tag="PL000_06160"
     CDS             complement(31005..32348)
                     /locus_tag="PL000_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88514.1"
                     /translation="MKPPLFTALLVIVFLVTACAQSPAPLATATIVPTPYPNVLFVDP
                     GISLGQISPLVYGTNYGPWIALSVEGLQPAYDSGVSIVRFPAGSWGDHNNVQTYQIDQ
                     LMSFIEKVGATAMINVRLLGGTPEQAAEMVRYVNKEQKYGVVYWGIGNEPSLYDGELK
                     NRGESYDIERFNQEWRAFAKAMKKVDSSIKLVGPETHQFSFDYNGASTNFSEANELWM
                     REFLKSNGDLVDIVSFHRYPFPRSFTSGPASIEELRVNAREWDKIIIHLRELIHTETG
                     RDIPIAVTEFNSDYSKSVGGESTPDSHYNAIWLGDVLGQMIKNGVFMANHWMLTSKGG
                     NGGWGLIAQTDVNPSYFTYQMYKKFGDELVYSSSDIADLTVYASLRDDGALTVLVVNL
                     SLEEQIQSLRIAGQAAVKGEAWLFDPVHQAENIGTVDLSGNHSFPAQSITLFIIK"
     gene            complement(32370..32999)
                     /locus_tag="PL000_06165"
     CDS             complement(32370..32999)
                     /locus_tag="PL000_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88515.1"
                     /translation="MKKFLILIFLLASCTPPAKTTTQPQIVTAYSTSAANPWLVELFS
                     CAESLNTAINISADSPDILLRVGEPEPLVSPAYQVGKDELLVIVHQDSPLQDLTLEEV
                     QSLFSAQENSTTQVWVYASDADMQVLFEQAVMEGRIVTSFAKVAVDSQRMLEAVESES
                     NSVGFILRGSVNSGQAYREVYSAGDVPVLALTQVEASGIIRDLISCISK"
     gene            complement(33030..34124)
                     /locus_tag="PL000_06170"
     CDS             complement(33030..34124)
                     /locus_tag="PL000_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNC88516.1"
                     /translation="MMKNLLKLLVETESPSHDKAAVDRVGKIVAEECRKLNAQVEILP
                     NEQTGDHVLARWGGTGKGGPLLLCHMDTVFPKGTTQTFTYKEVDGKIHGPGTLDMKAG
                     IVIALGGISALLSKGTAVPHPITALFTSDEEIGSFTSREYIEKLASEASIVFVMEGAL
                     MDGSLKTWRKGVGEFRVKVKGRAAHAGGDHKEGRNAIEEMAHQVIAIQKLTDYEKQTT
                     LNVGIIHGGTVSNVVPEEASIVVDVRVMQPGEWERLEGEMKKLKPVLDGTSIEVSGEL
                     NRPPLPFNEINQAAFKKAAQIAKETLGLELKAGGTGGASDGNFVAPLGIPVLDGMGAI
                     GEGYHSEREYIFADSLEERAKLLSVLIENW"
     gene            complement(34121..34954)
                     /locus_tag="PL000_06175"
     CDS             complement(34121..34954)
                     /locus_tag="PL000_06175"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC88517.1"
                     /translation="MSLFDVFILGIVQGLTEFIPVSSTAHLLVAQNLLKIPATDAAFS
                     FSVLVQMGTVVSLFFFFWKDLLSIALDTLKNLGNLKNFEQMPQNAKLGWYIVIATIPA
                     LIAGFFLRDSVQALFKTPLIEAAIRLFTASFLLAMAEFLDKKTRGLETMTWLDALIVG
                     LFQIIAVFPGASRSGTTIAGSMVRGFTREDSARFAFLMSIPVMLAAGSYEMLSVIERS
                     GTRAFLPYLLAGFLTSAAVGWLAIKWLMSYLHNNKHSLYVFSAYCGVAGIAVLFIQKL
                     P"
     gene            complement(35203..35940)
                     /locus_tag="PL000_06180"
     CDS             complement(35203..35940)
                     /locus_tag="PL000_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88518.1"
                     /translation="MSKEKSFFRPIFYVVVVIYFILGFCTLALGEKWGDKLFLEDTYF
                     ENVGALSLGIASVISFYIFQQALKARTTANIHWFKLLVYLGLAMLYFFGAGEEISWGQ
                     RIFNIPEPENLPIENVQEELNVHNLAVFENSDILKADNIFTVFWVGFAAMLPALSLAS
                     KPFKAFAEKFTPIVHWDIGLLFIFNYLAAQVAKPIFRSFYSYQTISFVQAVQEVKESN
                     YEFLFIFLSIAVLWDLRQLIGKSGTSK"
     gene            complement(36097..37221)
                     /gene="tgt"
                     /locus_tag="PL000_06185"
     CDS             complement(36097..37221)
                     /gene="tgt"
                     /locus_tag="PL000_06185"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC88519.1"
                     /translation="MFSFQITSKNNRARTGIFSTPHGDLVTPVFAPVGTQATVKTLTP
                     EHLKDIQASLVLSNTYHLYLRPGDELVRDMGGLHKFMQWPNPMLTDSGGFQVFSLSNT
                     RKIDDDGVTFKSHIDGSTHRFTPEKSIAIQENLGADIIMAFDECSDPNDLAYSKIAMD
                     RTHRWAERSLKAKTRPDQALFGIVQGGVNADLRAESAQFIASLNTPGIAIGGLSVGET
                     KQEMHDTLDVVMPLLPENKPRYLMGVGTPEDLINGVLRGVDVFDCVLPTRLARHHSAF
                     APEGRLNLMNATFARDERPIDETCDCYACKTFTRAYIRHLIVAKELLAGTLISIHNLR
                     ALIRLMEKIRLYIAEGTFESRAPELLDQWSNNAKRAGSGA"
     gene            complement(37251..38549)
                     /locus_tag="PL000_06190"
     CDS             complement(37251..38549)
                     /locus_tag="PL000_06190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNC88520.1"
                     /translation="MDSTFHTAEGIELYNASIMEFNNSLSQLPNPSAKRIALRISPPA
                     ERALRQGHPWIFDQSITEQSHQGAPGDLAVIFDDKRRFLAIGLYDPTSPIRVRILQSR
                     DPATINTEWFQNKLKIAAQTRAHLEKEKTTGYRLVHGENDGLPGLVIDRYADTLVLKL
                     YTPAWIPHLKSFCDALAQVNTASHLILRLNRSLQKQTEFLHHLNDGMTITDQTSPSLI
                     LFQENGLTFESDPIHGQKTGFFLDQRENRTRVEKFSKNKTVLNVFAYTGGFSVYAARG
                     GAKEVTSVDISPPAIEAAIRNMKHNQHFPTVASAQHETFAEDAFEVLARMQSQKRLFD
                     VVIIDPPMFAQSEKQTAGALSAYRKLTQLGLGVLRRGGMLVQASCSSRVDSDSFFESI
                     HRSAQEAGRSLIEIERTGHALDHPIRFIEGAYLKCLFATA"
     gene            complement(38542..>39029)
                     /locus_tag="PL000_06195"
     CDS             complement(38542..>39029)
                     /locus_tag="PL000_06195"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNC88521.1"
                     /translation="GFVAWRLCVYPKDLATPTKFYRDIIPSMDVTLTTPALLFPTVSL
                     LMLAYTNRFLTLASIIRNLHEKYKTDHNENLLGQIANLRYRVYLIRNMQIVGVLSLLF
                     CVVSMFALFAGWTLGGNWSFGIALILMIISMLISLRELQVSVGALDLLLMDMEEHEKF
                     G"
BASE COUNT         9125 a         9484 c        10689 g         9731 t
ORIGIN      
        1 tttttcctcg cgatttttat gaagttaatt cttttgtaat tttgatcaga tccatcgcat
       61 cgtcaattaa ttcaaaatga cctgcatcga ttctttgatc gagcgcggat tgaatcacct
      121 gcacccactt gccgcccagt ttctcctgtg tccatttcgc tgcgacgggt tttgatacga
      181 tggttccgtg ttcgaacgcg tgtagtgcgc ggcacatggt gaggatggcg tatgaatggt
      241 agtggctgtc atgctcacgc agccaggatg ggtctttcaa catggggaac caccattcct
      301 tgagaatgcc tgtcaccgct tttcgaattt cgtctgggga gacggggtcg atcaaagttt
      361 ttgggtctgg cccttcgagc gctttcccat attcgcggat gatatgccgc tggatgatcc
      421 agtcgctgcc atgtggcgcg acgtaaaatt ttgattcgtt gatgcttggg taggcttcgc
      481 cattcgcttc gtaccgccgt aagggcttct ggggaatata tgagccttcg agtttggacg
      541 cccattccat gccgcttttc cagagttcat tgtgcatggt ttccagtttg gaaattgctt
      601 cttcgggaag cgcctcgcag gtgacaatga gaaagtcaat gtcgctggca tcggggtgaa
      661 aatctccgct ggaaagcgag ccgtataaat acataccgac gaattgatcg cccaaaatgg
      721 tttttgcttt ttcgagcaaa gcagacagaa tttgattaac gccatcgtgt ggcatgaaat
      781 tttgtgttga catgaatttc tccatttaca aaacgcggac tgaagtccgc gttccgattt
      841 ttttatttcg atatctttcc atcttttgcc acccaaacat cggaggcgaa gcgatcaatg
      901 aagctgcggt catggacaac ggcaaggatg gttcccttga agttttcaag ggcttcttcg
      961 aagcgttcac gtgaggaaat gtcgagatgg ttgatgggtt catccaaaac aaggaaggtg
     1021 catccctgtg caacaagggt tgccagttgc agccgtgcgc gttcgccata ggaaagttct
     1081 tccgaggctc gcagggcggc atcgcctttg aagaggaagt aatgcaggaa gttgcgcgca
     1141 tccgtctcgt tgaaagcaga cacagactga atggtctgca gcgcgctgaa tttcgggttg
     1201 agcaattcct gctcctgtgc catgtaaccg agctttgtcg cgccgcccaa acggaacgaa
     1261 cccgctagtg ggggaattct gccgacgatg gtacggatga aggaggtttt ccctgcgccg
     1321 ttgggccccg tcaacacaat gcgttgacct gcgcgaataa acaggttgat attttcaagc
     1381 agtggacttt ctagcgcgta gccaatagat agcgaatcgg tttccaatac aattttcgat
     1441 tgatgttcag gctcgccgag ttcgagtttc atgtgccagc cttgcaatgg cttgtccaca
     1501 cgttcgtctg aacggaggta gcgatccaat ttcttttctc gtgattttgc tttaacagcc
     1561 actttctttg cgattcttct cacgccgggt tggcgcgggg tggtggtcaa ttccacgtgc
     1621 agggcttgtt gtttcgtgcg gttgatgtcg tctttcatgc ggcgaatttc agtttcctga
     1681 tcgcgatact cgtcccattg tttatcctgc tctttttgtt ttgcctccat ataatcgctg
     1741 taggtacctg catacatgcg aatgccgtgt gtgatcggat tcaattcaag gatggaggtg
     1801 actgtgttat cgagaaatac gcggtcatga gagacgatca atgccgcgcc ttgaaattga
     1861 ttcagccatt cttcgagcca ttcgagcatt tcgatatcga ggtggttggt gggttcatcc
     1921 agtagcagca attgtggttc ttcgagcagc acgcgcgcga gcatgaggcg ggtcttttgt
     1981 ccaccgctga ggtgggcgat gggtgtatcg gcggggagat ggtcaaggcc gagcggggcg
     2041 aggatggttt caggttgcag gtttaaggct gaaagttttg taagtgcgtg gtcgtattgt
     2101 gtttgcaggg ccgagtcatt tgggttttga gataaggcga gggcgagcga ctctatttca
     2161 gcggcgggat ctgtttgggt ggcaggatca agcccgagcg cagtatgaag cgtctgcctg
     2221 gggtcgaggt ccatgccttg ggcgagatat ccgatcctca agttggggcg tgtcgaagag
     2281 acatccccgc gccctgcgtc aggttgttcg agttttgcga ggatgcgcat gagggtggtc
     2341 ttgcccgatc cgttcgggcc gatcagtccg atgcgctcgc cagcgctgat gttgaagttg
     2401 atattttgta aaacgggttg gataccgtag cttttggata ggttgtgaat actcagcata
     2461 atgacgttcc tgtagttaaa aagaaaaccg agggcatgcc tcggctgatg cttttccaat
     2521 agatcgatgt catcggaatc cacagtgggc gattggatgt cgccactgct aacagcgggt
     2581 tggtgccctg aaaaaactgg ttgtttacag aatatccgtc attcgctggg tgctccttga
     2641 taggacaaaa ttttatcata ctaaggtaaa ctttggcgtc tggtggacgt ctatgcacga
     2701 gaggaaaatg agatgacaaa aactttactt gcggttctgg ctcaccctga tgatgagtct
     2761 tttggtttgg gcggcacact ggccttgtat gccagccgtg gctatgatgt gtattatgtc
     2821 tgcgcgacgc gcggcgaagt tggcaccgtg gatgcggagc atttgaaggg tttcaaagat
     2881 accgccgaga tgcgcaccga tgaattgatg cgcgcaggca aagaacttgg tttgaaggaa
     2941 atttttttcc tcggttatcg tgattcgggt atgcctggca cggaagataa caagcacccc
     3001 gatgcacaga tcaatgcgcc gatcgatgag gtagcggggc gtgtggtgaa acacatccgc
     3061 acactcaagc ccgaggtggt tttgaccttt gacccgatcg gcggatacaa acatcctgac
     3121 cacattcata ttcacaaggc gacggtgctt gccttcgaaa aggcagacga cgcatctttc
     3181 caccctgagg ctggtgcggc tttcaagccg aaggccttgt actttcaggt ttttccgaaa
     3241 ggcctgctga aggcggctgt gcgtttgatg ccgctctttg gaaaagaccc gaccaaattt
     3301 ggccgcaacg gcgatatcaa tatcaaggaa ctggcggaag tgaccttccc gatccatgtc
     3361 cgtttgaatg tggcttctgc ggctgaggcg aagcaaaagg cgagcgcgtg tcatgcctcg
     3421 cagggcggag gccagatgcg gcgcggattg atgggctttg taacgcgcat gtttggcgaa
     3481 aacgaagatt atatgcaggc ctatccgcct gtggctggga gttcaaagcg caaaacggac
     3541 ttgttcgaag gtatttagtt tcgttgcatt ttcaaaaatg taggttgttt gtacctcttg
     3601 atgggtttat acttttcgta cacaacaagg aggttgttat gaagcacctg cgtttgctct
     3661 ttgtttccct ggtccttgtg gcgcttttgg ctggcacagt gaccacccca tctttggctt
     3721 atcccgatag tacaaccacg atcgtgaagg ttcccgttaa gcccaatcgg tcactgccga
     3781 atgcagatac ctacgactgt accaaggcaa ccctgtataa caggaatggc agttacgcgg
     3841 ctacctttac aaatccgcct gcgagcaacg gctgcacctt cattttcaac aacgttcctg
     3901 tcaatcagtg gtacacggtt gagttcctga tgaagtacaa attgggttcg ccgccttata
     3961 ttgtgatcag gacctcgcaa tcagtttata ttgcaaagcc tacacttacc accatcacaa
     4021 cagcgacatt gctttatcgt ccgaaatgat tttgaaaaaa tagacatgtt tggtagtcaa
     4081 aacgactggg aaccatttcc agtcgttttt cgttgattcg ggtgagaatc cctttttgga
     4141 aagcatgata gaattttctc aactccaaac tacaaaaaaa acactatgac tgaatcaaat
     4201 cctcctcttc caacgcgccg attcaatttt gcgcgcattc tcgaagtttt gcttcatccc
     4261 aaacgcgcct ttgcagaaat tgcaggagag accgtatctt catggctaac tccgatgctg
     4321 gcgttgagca tttcgtcttc cctggtcgtc attgtgagtg gatttttgaa agcgcgtgca
     4381 gcagcgatgg gggagatcca actgcctccc gattggcagt attggacccc tgatatgcag
     4441 aataacttca tgcaggccca gcaggccaca cagggagcga cttttgtgta catcattccg
     4501 ctgctttcag cgctggctgg tttgtggctg ggatggttga ttttgagcgg gctgctccac
     4561 ctcggctcca ctttgatggg cgggcgcggt acgatgggca gtgccctgaa tgttgttggc
     4621 tggggtggtt tgccattttt gctgcgtgat gtgctgcggg tggttttcat gctgatcgtt
     4681 gggcatacca ttgtcagccc ggggttatcg gggtttgcgg gtggggctgt gttcctgagc
     4741 aaaatgcttt cacgggtgga tattttcgtt atctggaatg ccatcctctt aattcttggg
     4801 tttggcacag cagatggcct tacaaggaat aaggccatcc ttgtagtgat cgtcattctg
     4861 gtggtcattc ttcttgcatt ggctggggca gggacattgg tttccagttt cagcagcggg
     4921 gcagcgttct aattccattt ttgcctatga cctcgcctct cattcaaatt cgtgatctga
     4981 aaaaacatta ccagatgggt gggacgaccg tccgcgcatt ggatggggtt agcctcgata
     5041 ttgagccgca cacctttacg gtggtgatgg ggccttcggg gtcgggtaag agcagtttgt
     5101 tgtacctgct tggcgggttg gaccgttcca cttcgggcga gatcacgatc agtggtgagc
     5161 gcctcgacca aatggatgaa aacacgctcg cgcttttccg ccgccgtacc atgggattta
     5221 tctttcagtc gtataactta attcccagca tgaccgcatt ggaaaatgta tcttttccca
     5281 tgcagttttc gggcattccc gtttcagagc ggaatagccg ctcgcgtcag ctgctggaac
     5341 aggtgggctt gggggaacgc agtcatcatc gtcccaccga gctttcgggt ggtcagcagc
     5401 agcgtgtggc tgtggcaagg gccctggtga atgatccgag cctggttctc gccgatgagc
     5461 cgacaggaaa tcttgataca gccagcgggg tgaccgtgat gcagttgctt tctgatctgc
     5521 atcgttcagg gcgtaccgta ctggtggtga cgcatgaccc gcgcatgacc cgttttgcta
     5581 cacacaaaat attcctgttg gatggtcatg tggtgacgga agaggaatat caatctgctt
     5641 cgatggatat gatgcagact ttgtcagatt caccttccgc gtaaattgaa tcatgggaga
     5701 gtacatgaag ttccaaagta aatattttgt ttccattctt ctggcagccg cttttttggc
     5761 aagttgggct acgcctgttt acgcagaccc gatcatcagc tcgatcgacc ccaatcttgt
     5821 gttcaatgat gctcccagca ccgttacgat cacggggtcg gggtttgaat ctggtgcggt
     5881 ggtttcgttg aaaaattacg gtgacctcac cacctcgttc gatggtccca accaattgcg
     5941 ggctgtggtg cgcccggggc tgcctgcggg ttcttatacg gtcgtagtca ccaacccaga
     6001 tgcgaccacc tttgagtacc ccaacggact gaccgtagcc gatacatccc ctttacccac
     6061 accgacctcg gcgccttttg accgtccaca aatggtcatt ggttcctatg aggtcaatgt
     6121 gcagcgtgtc cgttcaggag atgatttcaa acttaacatt gattttggca atgctggcac
     6181 tgtggctgcc tacaatgttc aggctgtttt cacgtctgct gatttgattg ccaccaaaac
     6241 gggcggtgtc atctttttgg gcacgatccg tgcgggtgac cataatgacg ccacccagcc
     6301 attcatcgcc ctcgactcta tttatgggaa atctgtcgtt accacagatg taacgctgac
     6361 ctattatgac agtgccggca ccgagtacac cgataaattt acgctcagca tccccgtggg
     6421 aggttccaat gtgccgtacc ccacggcaac ccccacaggg gtgaacactg cccaattggt
     6481 catcaccagt tacgggtcca cggtggatcc gttgcagccg ggagagcagt tccagcttgg
     6541 gctgacagtc caaaatatgg ggaacaccag tgcgaagaac gtcaccatga tcgttggggg
     6601 cggttcctcc ggtgatagca gcggcactcc ccagcctggt ggtgtttcgg gcagcggcgg
     6661 tgaatttacc aactttgcgc ctgtcaattc ttcaaacatt caatcgcttg gcgatcttcc
     6721 cgcaggcgga cagttgcagg tcttccaaag tttgatcgtt aatgtatcca ccaacccggg
     6781 cgcctacccg atgaagatct cgttttcata cattaacaac aagggcgaag tggttaatga
     6841 cgatcaggtc atcacgttgt tggtgtacag ccttccaaat gtggatgtga gtttctaccg
     6901 cccgcccgat ccgttttttg cggggcagcc cggggcgttg ccgattcaga tcgtcagcct
     6961 cggaaagcgt gcttctgtgc tgggaaatat gaaaattgaa acgacaaatg gaatggtcga
     7021 tcctgccaca accttggtcg ggtcgttgga cccgggcggt tacttcacct ttgatacgac
     7081 cctgatcccc gatgtcgctg gcccgatgga attgacagtc accatcgaat acaccgacga
     7141 tttcaaccaa ccgcgcacga tcacccgcaa attgcaagtg accgtggagg aagcctttgt
     7201 agaccccgcc cttgaccctt cgcttgatcc ctcactcggc ggcggaggcg gcgaatttat
     7261 tcccatggaa gaagaaaccc tcctccaaaa gatttggcgt ggaatccttg gcctgctcgg
     7321 tttggattcc gcttcacctg caaacaattt ccccacagaa ggccccgccc tggaaggtga
     7381 gcccctgccc atgcctgcaa atggcggcaa aggcggttaa tcggatatga aatttctcga
     7441 cctgctccgc ctgatcttcg gcaatcttgg ccgccgtaaa gctcgcgtag cgttgaccgc
     7501 catcggcgtg gtcatcggta cggcggcggt ggtcatcctt gtttcattgg ctgttggcct
     7561 ccaaaagagt gccaccgacc aactctacgg catcggtgac ctgaccttga ttgatgtcat
     7621 gcctgcctac ggtgtatttt atggcggcgg atatggcgcc ccgatgatgg gcggcggcgg
     7681 ggggggaggt ggagggttgg atgaaccagc tctcattaca aactcggtcc tcgaacagtt
     7741 tcgcgccatc cccggcgtcc atacggtgat cccacgtgat tacctcaatg cttccaccat
     7801 catctcttat cgtaaattgg aagcaggcat gggcttcatt ggcatagata cggatgacct
     7861 tgccaatatt ggtctggagg ccagcgaagg cacaaccatc ctttcaagag gttctgtggt
     7921 gatcggcgcc atgacgccca acaacttcta cgatccaaac ctgcgccccg ggcaggagcc
     7981 gccccttcca cccaatctgt acaacgagca aattcaaatg gtcgtgatga agtgggatca
     8041 ggaagggaac gtatcgcaaa agatcatcag tttgcgcgta gcgggtgttc tcaccgaaac
     8101 gggcggcgaa gcggattatt ccatctatct tccgctggac ctcgtgcgaa gtttgaacga
     8161 atgggcaaac ggggtgcgca tcaactacaa caagactggc tacaaccagg tcattgtcaa
     8221 ggtggatgac cccaaccagg ccctcgatat tgctgaccag atcaccgcaa tggggtatca
     8281 agccattacc ccgcaagcct acctcgaaag catcaatggc tacttccttg ttttgcagat
     8341 catctttggc ggcgtaggga ccattgcatt gctggttgca gccattggca ttgccaacac
     8401 aatggccatg tctattttgg agcgtacgcg tgagatcggc ctgatgaaag cagttggcgc
     8461 aaccaaccgc gatgtgctgt ccatttttct tggggaggcg gcaggtatcg gctttattgg
     8521 aggcgcgggt ggcgtcatca ttggctggct ggctgcacaa ggcatcaacg tgcttgcgat
     8581 catctatctg gctgggcagg ctagtgaaca gggcggcccg cctcccagtg tggcggtcta
     8641 cacccccgta tggctgcccg ttttcgccct catcttcgcc accattattg gaatgctttc
     8701 aggcctgtat ccagccctgc gtgcagcgac gatgatccct gtgcaggctt tgaaatacga
     8761 ataaaaccga cgcttcacac tccgccccaa cctgcctcgc tttcgaggga aattttctcc
     8821 ctgtaaaatt tccccgccat tctgcagatg tttcttgaaa aattaatata aaacgtatat
     8881 ttttcacctt catccataat ggtaggatta tgcaataatc attgccttat gaaggagaat
     8941 ttccatgcca gagcgtcgaa ccaccccccg aaaaaagttt gattattatt tgcgagttgt
     9001 agatgatgac acacagggca ttcttggcca catggtcgag gtcagcccca ttggtctgca
     9061 gcttgaaaca acagctccct gcccggttga aaaagactat tacctgcgtg tagagctcac
     9121 ccccgaactg gcagacaggc cgttcattat ttttattgcc cgctcaaaat ggtgccgaat
     9181 tgatgagatc gagccgaatc tgtatcacgt gggctttgcc atggcagaga tcatgcctga
     9241 tgacagggaa attttcctgc gcatccttgc gcgttttggt cgataaacga ccaaagtaca
     9301 tctggggggc acattcgaaa agtccccaaa tatggcgctt gacattttag aacacgcgtt
     9361 ctaaaataaa catacaatta cccactttaa ccaaaggaga atattgctat gtccaagcga
     9421 aatattgttc atgttgagat tcctgctgcg aatgttgaag gtgcaggcaa gttctaccag
     9481 gaattgtttg gctggaagct tcaacacatg cctgaaatga actacaccat gtgggaagca
     9541 ggcgatggct ctggcggcgg tttccctgct gtttcggctg agaacccggc ggggcaggtt
     9601 ctggtttaca ttgacagcga tgatatcgaa gccgacctga aaaaggccga aaaattaggc
     9661 ggcaaagtga ttttccaaaa gacggagatc ccgggtatgg gctggtttgc ggtttttcat
     9721 gacccaaccg ggaatgccat tggtctttac acaagtttga atcctgcaaa ttagcgaggc
     9781 gtacaagcac atggctaccg gcgattttgc ttttgtgacg agcctcgaac ctgctcagaa
     9841 agatgtgttt ctcaacaggg agaagtatga cattgtgtac gcctccatac ttgagatctt
     9901 gcgcatgcac ggccccatga cctttaccca acttgggcat cgtgtggagg aacggctgca
     9961 ggatggtttt gacggctcag tgttgtggta ctttaccatc gtaaaattgg atatggaagc
    10021 ttgcggcgag attcagcgcg tggtgaattc aaaaccgcaa ttgttctctc taaatgtcaa
    10081 tgtctgatcg ttcttgatga ataaaacatc ccgcggatga aatctgcggg atgttttgat
    10141 tcttgagact gctgaaaatt tcagcctgcc cacatatcga acatgtacat gatgcacatc
    10201 atactcatga gcagcatgct ggcaatggtc cagatgcggt caaagatggg gtttttcccg
    10261 agcctgctga gaaagagaaa aacaggcggt gcggcaagaa catagcgata catgccctgc
    10321 gcgggtccgc tggtgaaaga gaggaagacc acaagaaagc cgaagatggc gaggtctggg
    10381 tgtcttttga aacctgcaat gcaggcagca aacccgatga tgattccaag ccattccacg
    10441 aaatagtagg cggctgcgtt ggggttgttg ccgaagatct ccataaagcc atctttccag
    10501 gcaatgaagg tgtatccaag cgagagcagg ccgcgcccga agaattcttc ttccacaagg
    10561 ctgaaggcca gcccgtagta cgatatccgc cagaggaaga aagcgatgat cggagcagtg
    10621 gcaatcaacc cgttgacaag gatgcgccag ggcaggccgc ggtagtagat ctgcttccat
    10681 tccatgtcga gttccagcca ttccgcatct ttgatccatg aaatgaaaag gggaatggcg
    10741 agggtgacgc ccacggcacg tgtaaaggtg gcgaggacag ccacaagagc cgcccagccg
    10801 cgatgtccac gccgaagcag gagcagactg ctgaatgcca accccacgaa caagccttca
    10861 gtatagattt cagcaaggaa gaagccgctc ggaaaaataa gcagataaaa ggcggcgcgc
    10921 agaccgctgc tttcatccag ctcggtgcgg gcgaattcgt agagtgcgat catggcggcg
    10981 agtgtgccga gcatggaaac gatcacccct gccagggagg cggtggcgat ggcattcatg
    11041 ccaagcagcg aaagcggaag cgcgaacagg cggatcatca tggggtagaa cgggaagaag
    11101 gcgtaactca atgatatgcc cgggcgttcg gcagattggg gagggacgac gaacggccag
    11161 aatcctccgc tttcggtggt ttcgccagtg ttggagcgaa tccgcgtggg gctggggtct
    11221 tcatagccgc cgacagcgat ggcgagataa tattccgaat cccatgaaac gtggcggttg
    11281 aggaaaggtt cattcaggta gatctttcca tattgactgc ctggcatggt ttcggtgacg
    11341 gtccattcga gggcgcggtc tggcggttga acggtgaggc gggcagggag caggttgtga
    11401 tacgccagca tgatcaatgc ccatcccagc cagattccga agatatttcg gtgattcgga
    11461 tttttgaaca tgggtctact tttccattcc tgtcaccacc atgtcgcgca tgaagtaacg
    11521 ttgtgaaatg atcaacacaa tgacggggac gatcatcaaa atgagcgcgc ccgcttgaag
    11581 ctggttttga atgggggcga ggctttgaaa actttgcacc ccaaaggaaa tgggagcaag
    11641 gtctctgcgg gtgctgaggt acagggctgc ctgtcgggtt tcgttccaca tgtagtagaa
    11701 atgcagcagc gacacggtga ggacggtagg ccacgattgc ggcaggatga cctgaaacaa
    11761 aatccgcaat ggaccagcgc cgtcaagcat ggcagcttca tcgaggtctt ttggaatgct
    11821 cttgaagttt tgtctcagca aaaagatgta aacggcattc ccaaagaaga acggaatcaa
    11881 aatgggcagc caggtgccct gccatttgag aacgcgcgca aaaaagaaat aggttgggat
    11941 gagcgtgacc ttctcgggaa ttaatatggt ggcgatcagg acataaaata gaaaattccc
    12001 tccaggcaat gggaagcgcg aaaaaccata agccactacg attgatgaga gcaggacccc
    12061 gatctcgctg atgatggtca tcatgaaggt gttttttacc atctcaggga tgcgcaggga
    12121 actgaacaga attttgaaat tatcgaatgt tacgtgaaaa cgatagacgc ctttcaactg
    12181 gcgccaactg cccttccagg gaatgagacc atcttcgggg ttttgcgggt cgatgaattt
    12241 gctggaggtt tgttttggct ccaccagagc cagctgacgc actccgccgt tgatgggaac
    12301 gttgtacacc agatattcct cgccctgata ttcaaaacgg acctgttgcg atgggtaggc
    12361 gggggcattg cggtcacgga gttgaatccc ttccatgagc gaagtggcaa ccatgaaaag
    12421 cattggaaaa agataaacga acaacatgaa aaagatgaac aggttgaaca aggcactgcc
    12481 cagcgtttcc cacaattgca gcgatagata attatgcggt tcaagccatt tgtgtctgga
    12541 ttttgttttc atgcctaggc ttccctgtct gggtagtaga cccaattgcg ggcgcttgaa
    12601 aacagcgcca cgaccacgat catgacaaga atgaaaaaga accacgccag actggcggca
    12661 tagcccagat ccattttgtc gaagaggatg taactgacat acccatcgta aggtgaaaaa
    12721 ctgccgctga atacgctgcc gcgatcaagc agaaccacac cgccaaatac tgcgatcatg
    12781 ttgatgatga tcgaaaagaa aatggcagga gaaatgagcg gcagggtgat gtagaaaaaa
    12841 cgcaccagcg gacccgcgcc atccacccgt gcggcttctt gaatttcagg cggaagccct
    12901 tgcagggcac caagcatgat gagcatgcct ggccccaccg accagagtga gctaatgctg
    12961 aagagcagtg ccacggcgcc atcggagtac aggttgttga atcctgtcag cccaagcgga
    13021 tgcagaatga gcttgttcaa ccatcccgtc gttggatcgg cgaagccaat ccacatgaag
    13081 aaaatggcta caccggggat gatgctcggc aggaagaaaa gagtccgcat ggtgttgccg
    13141 aatttcaaac gcgggttggc aagcatggat gccagtacga tggatgccgc caattgcaga
    13201 gggatggttg aaatgcccaa tgcaatggtt tcaaacaata catacccaac ggcttcatcc
    13261 tgaaacaggc gggcgtaatt ttcgagcccg acgaactgtg tttgctcggg ggtaagcagt
    13321 tggaaattgg tgaatgaaat tcccagcgaa gccagaattg ggagtaattt gaaaaatagc
    13381 agaccgatga cccagggagc gatcaatagc aggccaaacc gtctgtcata tctcaagcgt
    13441 tttgtatttc cagtggacat ggtttccttt gcagatcttc cgataaattt agcgtgggct
    13501 ttgagtgata tactcctgcc cgatcaaact atgaagcctg aacaaaaaag ccgattcaac
    13561 gatctcttct ttgcgcgttt gtattatttt tgtttcatgg gaggttgggg atttgttctc
    13621 ccattcatca atctctttta tgtcagcatt ggcttcaatg ggaaacagat cggggttatt
    13681 tcatctgtca gcgccattgt gggtatgctg gtttcaccgc tgtgggtgag tgaggtcaaa
    13741 aagcgcccac aagctcgcaa tattttgcag gccgctttga tccttggcgg tattggctac
    13801 tatgccattg gtttgcagag ccagttcctg tgggtcgtgg tcatcatatt tttacatgcg
    13861 cttgcggcat cgggcattgc gccgctttcc gactcgatgg ctgtgaccgt atcacaggag
    13921 gcaggcagcg gatacggaag cgtgcgcgtg tggggatcgc tgggatggat cttcacggtc
    13981 ctttcttcgg gatggctgac agcccgcttc ggctttatcg ccgcttttat cggagtggcc
    14041 ttaatgtggc tgacagccgc cagcctggtc tttttaattc aaccgcgctt tttcagcgca
    14101 cagcctgcat ctgcacaggt cagaccaaac ttatctgctg cacttcaata tatctggcat
    14161 gacaaagtcc tgcgcggatt tgcgctggcg ctcattatct tcggcatttt caacaacggt
    14221 gttgtgcagt tcgaaaatgt tttcctggcc gaactcggcg ccaccaaaca ggttatttct
    14281 gtggcgggca tcatgagcgc attggttgag atcccgttca tgttgttctc tgaccgaatc
    14341 gttcaacgag tgggggcgca ccgtctgctg atggttgctc tcaccatgac gatttttatg
    14401 cgcctttcgg tgctgacctt ccccgccatt cttacgatca tggcggtgcg ttttattggc
    14461 ggggtggctt tcagctttta caccatctca tttatcaaac tcgtcagcag ccgcacacaa
    14521 cccacagaga caggtacggt gctggcgatt ctaagcgtga ccattacagg gctggtgaat
    14581 atcatcgctt cgccgatctc gggcgcgatc tttgatgtca ttggcgcccg ctggttatat
    14641 gccttgtctg ccacaggcta tgtgatcagt atcgcctgcc tgtggtggac tcggccagag
    14701 aaacagcccg cagggtcggc aggctaaaat ccgttcttgc ggatgacggt tttgtaccat
    14761 ttggcgctgt ctttgaggat gcgttcctgt gtttcataat tgacccagac aatgccaaag
    14821 cgttgggcat acccaaacga ccattcaaag ttatccatca acgaccagac gaaatatccg
    14881 cccagcggta cacctgcctg catggcgcga tgcgcggcgg caaaatgggt gcgcaggtag
    14941 ttggtgcgat gcacgtcagg gacattgcca ttttcatcag gcgctgtgga ataactggcg
    15001 ccgttctctg tgatatagat ctttagcggg gtgtaatcga aatacacacg tcctaaaatg
    15061 ccagtgagac cttcagggta gttctcccaa tccatctctg tccaatgttc aggggttttg
    15121 gggtgtggga agaagagctg cggaccatta ttcgggtctt caacgcggtg caaattgcgg
    15181 ccgtaataat taagaccgat gaaatcggtt gggactgcga tggtttccag atcgcctgac
    15241 tgcacaaaat ccaatccgtt gggggcgccg cccaactggg tgaaatattt ggtcatgtca
    15301 gatgggtaac cgcgcccgta gagcggatcg gcaaaccagc ggaaccattt gccgtcatca
    15361 aggcgggccg cgtccatgtc catcttgctg ttggaggcgg gcaccttcca gccgatgttg
    15421 agcgtgatgc caacttccgc atccttgctg ttgttgcgga tgacaggcac cgacatacca
    15481 tgcgagagca gcagatgatg cgcagcacgc agcgaagcag acaggtcttt gataccaggc
    15541 gcatgaaccc ccgtttcata acccagccag gctaccacgg cgggttcgtt gtgggtgatc
    15601 cactgatgga cacggtcacc gagtgcgcgt gacatgacat cggcatattc cacaaaggct
    15661 tcggtggtgg cgcgggctgt ccagccgcct ttgtcttcca aggcttgcgg cagatcccag
    15721 tgttcgaggg tgacgaaagg ggtaatgccc gcattgagca ggccatccac cagcttgctg
    15781 taaaaatcga gtcctttttg attgaccttg ccgcgtccgg ttggcagaac acgcggccat
    15841 gagaccgaaa aacggtaggc tttcagcccg agctttttca tcaacgcaat atcttttttc
    15901 cacagacggt aatggtcgtt ggctacatcg cctgtgtcgt tgttgcggat cttgcccgga
    15961 gtgtgggtga agcgatccca ggtggattca cccttgccat gtttgttcca tgcgccttcg
    16021 atctggtatg aggcggttgc cgcaccccag atgaaatttt cagggaaggt gattttgttt
    16081 gccattttct atgctccatc ttgatttgaa tgatcccgaa ggatggttcg tgcggtttcg
    16141 agcgtggctg ggtaactgcc tgtcagtacc tgccatgttt ctgagtgagt cactgagtgg
    16201 ccatgtttca atgtcttcag cgggccaagc gtttccagtt cgatgcatgt gtcttttacg
    16261 taaacttcca cattacagcc ctgatcgggg aaatctcccg cggtcacctc aaaacttttg
    16321 atgaacaacg catcgcccat tgcgcaggcc agccatccca atggggtgtg cgtgccgacc
    16381 ttgcatgctt cggcgctgac ctttccatga agcaggatca ggtcgtcgtt aagttcgagg
    16441 cgcggatcgt gaatccgcgt gtagggccaa agcacgagat tccgattcgg ctgcaaccca
    16501 tcggcattgg attgcggcac gatcgccatg ccgcccatgc gcatttgtgt gatcgcccac
    16561 ggcgcgaatc gaatttcatc tttgccgtgc caggtgatgc ggtgtgaaag atgcgcgcgg
    16621 ttttcatcca gccgaatggt gatttctctt tcaagggacg aagcatccac agggcttttc
    16681 aaaattaccg tgttgtggct gcgctccatt tgcaggccgt cttcgggacc catgaaaaaa
    16741 aggtcttcgg gcgcagtcat caaacgatgc ccgccgcgca gaaaaaactc gccgtgcggg
    16801 gtgggccagt gaatatcggg tgtttctgcc agcaggtttc cattcgctcc ccgcgcatac
    16861 aaaccgatga tgcgcggccc tgtcgtcgtg aaaaaatcaa gacgcagaaa atcgttttcg
    16921 atggatgcga tcggaagatg cgactcggcc atgactatcc catgaccacc cgaacatcaa
    16981 cctcggtctc accattttcc gcgcggatca aaccacccga tacctgcctg ccgttgactg
    17041 tgacagtttc cacccctttg cagacgtgcc tggggttttc cacggtgatg ttgtacgtga
    17101 cgccgcgata ttggcgggtt gcggtgaatc catcccaact ggcggggatg gcgggtgaaa
    17161 tttccagacc gtcgagtgtg gggcgaatac cgaggatgtg ttgtgtgatg gcggcataat
    17221 tccaggcggc agtgccggtg agccaggaat tcttggcttc accatgtgtg gcggcatctt
    17281 tgcctgcgat catctgggca tagacgtagg gttcgcactt gtgcatttcg ctgatctctt
    17341 cacgcgccga tggattgatg cgtttgtaat aatcataggc cgcatcgccg cgcccgacca
    17401 atgcttcggc gatcatgatc cacgggttgg tgtggcagaa gatgcccgca ttttccttgt
    17461 agccaggcgg gtaggacgag atttcaccga gatgcaaata atattggctg aaggcaggct
    17521 gttgcagcac aatgccgtgc ggagtggcga gatgctgggc cacagaatcc agggctttgg
    17581 cggcgttgcc gttttcaagc cccagcccag ccatgatgca gatgccctgc ggttcgatga
    17641 aaatttgccc ttcgctgttt tccttcgacc cgagcacatg cccaaaatca tcgtacgcgc
    17701 ggcggaacca atcgccatcc cagccagatt tccagatggt cgactccatg tcggttgcaa
    17761 atttgagata gtcgtccgaa gaaagatgaa agacgaaaga atcccctctt tcttctttca
    17821 tctttcctgc caggtctgcc atttctttac aagccagcac aaacaacccc gcgatgaaca
    17881 cgctttctgc caccttgcct tccttgttgg tggtggtttg gaaggattgc cccggggtgt
    17941 cggagaagca attcaaattc aggcagtcgt tccaatcggc gcggccaatg agggggagtt
    18001 gattggggcc aaggcggtca agggtgtatt gaatggagcg ttgtaaatgt tcgtagagcg
    18061 gggtttcggt gcccgcttca ttttcatatt gcaccatttc atcgagaatg gaccagtcgc
    18121 cgctttcttt gatgtaggcc gaaacaccca gcaccagcca aagcgggtca tcgttgaacc
    18181 ctgacccgac atcgttgttt ccgcgtttgg tcagcggctg gtattgatgg aaggcacctc
    18241 catttttcaa ttgcgtagcc gatagatcaa ggatgcgctc gcgtgcccgt tcgggaatca
    18301 tgtggacaaa gccaagcagg tcctgatttg aatcgcggaa gcccatgccg cgcccgattc
    18361 cgctttcgaa gtaggaagcc gagcggctca tgttgaaggt caccatgcat tgataggcat
    18421 tccagatatt gaccatgcgg ttggtgtgtt cgtcgggcgt gttgacctgt aacttgccga
    18481 gcagccccgt ccaatattcg cgcagggtat tgaaggcctg atcggcgttt tcgggtttga
    18541 ggtatttttc gagcgcgatc tttgcgcgct tcttgttgat gacctgcgat tgcggcgggt
    18601 cgaatttttc gtccttcggg ttttccacat acccaaggat gaaaacaatc tgtttggatt
    18661 cgcctgcctt caaattgact ttgacctgat gcgagccgat gggctgccag ccatgcgctt
    18721 cggtattgga cgatcggcct gattccacga cctggggttc gttccagctg cggtagtgcc
    18781 cgagaaaatc ctcgcggatg gtgtcgaagc ctgccagttt ttcggagcag gcaaaatagg
    18841 caaagtggtc gcggcgttcg cggtattcgg tcttgtggta gatgacctca tccacgatct
    18901 ccatttgacc gatggagtag ttgcgttgga aattcgtggc gtcgtcgttt gcatcccaca
    18961 gacaaaactc aatggcagag aacaggctca agtcggagtc tgatccgctt tcgttggtca
    19021 gggtcatctg ccagatctca agcgattctc caagtgggac aaaataacgg gtggaggatt
    19081 tgatcccatt gaatttagag gtgatgatgg tgtagccctg cccgtgtcgg cattcgtatt
    19141 catccagctt ggtgcgggtg ggcatccatg atggcgacca gaatttgcct gtgccgttat
    19201 cgcgcaggta gagatatcgt ccgcccatat cgagcgggga gttgttgtag cggtaccgtg
    19261 tgaggcggcg cagacgcgca tccttgtaaa aggaataacc gccagccgtg ttggagatca
    19321 gaccgaagta ggcttcactg ccaaggtagt tgatccacgg caagggagta tcgggttggg
    19381 tgataacgta ttcacgtgcc ttgtcgtcga agtagccgaa tttcataatt caaaaatcct
    19441 tttcactttc taggtatcct gctccacact gccaaagtgg atcaactcaa tgcctctgtc
    19501 tgcaatggcc tgccgaatgg ctggatcggt gagaatttca agctcggttt cgcgctgcct
    19561 tgcgtaggac gatgctttga ataacgcttc atctgcatac ccggggtgga ccatgatctc
    19621 gcaagtccca tcttgtatgg aggcaatgat gcgctgcaat tcggcgcggg tggccagttc
    19681 atcatagaaa ctggcgtaga aggtatttgc cctgcgcgga ttgaactcgg ccaacagggc
    19741 gggtgcatat tccttgatgg cgtcgtttcc ctccatgaac ggtaaacgga tggcgcaatc
    19801 gaattccttt gccagctcga gcatggcgcg gaaaagggcc gcactgaaat aggaactgtg
    19861 atggtgcgaa tccaagtgtg tcggcttttt gccagcggct tgaatgaagg cttcgatctg
    19921 tctgcgccac tcggctttta cttcactgac ctcgatgctg tggatgcgtt gagtcagcac
    19981 cgagaggttg aggaaattcc cagcttcatc ggtgatggag cgaaccgatt cgtgcggacc
    20041 gatgggcctg tctgctgtta gaacgaggtg gacgcccagc ccaagcaggg gtgcttcgcg
    20101 cagggccact tgaatatccg ctgcggtggt ggggatgttc atcatgcagg tggtggatgt
    20161 gaccacacca tgcagatgcg cttcgcgaat ccctcgagag acttcggggg ttcgtccata
    20221 atcgtcggag ttgatgatga gtcgtttcat aaggcgggag tgaaaatcag ggtcatggtt
    20281 atttgctcag gtctgtcaga tatttctggt acaggtcaac gagatggggc aggggttctt
    20341 tgtaaaattc gtccgcatcc acggtcaatt tggcgtaatc ggggatcggc aatacctgcg
    20401 gatttgtttt cgcaaattcc tgaatgaccc tggcatgagg cttgagtgaa ccatcagggc
    20461 ggacgaggcc gaagaatctt tcgtgttgcg aattctggca ggggggcata tcccacaact
    20521 cagggacata atcggcgtag caccagagca ttgcacccgt ggcgccgctg tgttggagct
    20581 tgggaattgt gagccgcagg aattcggcaa aatcttcttc gctggccatg aattgctcgc
    20641 gttcgcggcc attggtttcg gtccatttca tggtgtaggt ggtttcgcca ggcagggctg
    20701 tgcagccgcc gaattcttcc atcaaaacag gtttgccagc cagagcggcg gtcagtgcgc
    20761 aggtgaaagg gacaaaatca ggatcgagcg gttggcgtgc ccacggcgtg tacatggggt
    20821 aggaatgcat cacggccaca tcggtatgag cgtacacttt gtcaatgcgc aggccgttat
    20881 cgcggtgcaa gccatcgcca tgcaggccga tggtgacggg gtgattcggg tcaatggatt
    20941 tgatgaggcc gaccatttcc tttacccagg ccgcgccttc ttctgatgag ttgggccagg
    21001 cgaaaaggtc gggttcgttg ccgaggttcc acatccacac ggcggggtgg tctttcagcg
    21061 tagtgacgac ggttttcaac aacaggcgcg tggcgtttag ggccatttca tcatggaaca
    21121 tgttgcgata gcctttgtcg gtctttttgc cttcgctgac aatatcgcgg atccagttgt
    21181 gcgctttggg gggcaggtcg ccgcccagta accagcgcgg actccagttg ggtccgctca
    21241 tgtggcctgt gaagaaggtg atatcgagtc caagcccatg ttcggcggcg atatcggcaa
    21301 ccttcacaag gttttcgatc ttgtcctttg ccacactggc ggggtcgggt tgaaaatcgt
    21361 cccacagcaa aaacaggcgg acgacattta atccgatctc tttaataagg ctgaattctt
    21421 cgcgtacttc gcctgcatcg aaattactcc accagtacat ggctttgcgg cgcggccagt
    21481 agttgacacc gagtgtgaag ggttgtgaca tggttggctc cgaagaaggg tgagaggtga
    21541 ttaggggttc gtgtggatgg tttggtaaat ggtgttgacg agtgagtatt ggccttgtgc
    21601 atcgtgatcc aaagcccaga acatgattcc gcctgcattt tgcagggcga gttcggtttt
    21661 ggcttgcaca gtgggaatgc cgttgtaatt ttcaagagag ccgttgtatt cgaacgcatc
    21721 ggcctgtgcg gcggcgggat cgaactgcac cagctttgcg aaagtgacct cactaggccg
    21781 cgagtagaac ggtacgccga tatttatttt ttcctttggc acaccgcgcg cgctccagta
    21841 ttccaatcct tttttgaatt caacgagact gccgtgaatt tcgccgtcgt aggtcatcac
    21901 gttgacgaaa tcgacaagtg caaatgcctc gttgggaacg cccagcccaa tgtcatcacc
    21961 aaatgaaatg acggcggtgc tgatctcttt ttcgggcagg gcggcgcgca gttcctgcat
    22021 cagcgcgagg aagttttgtg ccgattgccc ctggtctgga tattcccagt caatatccac
    22081 gccatcgagt tgatattgct ccacgaacgc agcgagattg ctgacgaatg cgcttcttgc
    22141 ctcagggacg gctgccatgg cctcgaattc attgtcccag ccccagccgc ctacggcgat
    22201 attcacgcgg acgttatttt cgtgtgcggc agcgacgatc tcttcaagag cccaggcgtt
    22261 gttgatgttc acaaaactcc catccgcttt ggggatgagg aacgagtagt tgatgtgagt
    22321 caactggtgg tagggaattg cagagggggt gatggcgtca gtggcgtagg cgacgattcg
    22381 gaaggagggt ttctgggtca tggccgaatt ttgcggggcg caaaatgtca atgaaagggt
    22441 aagcaggcac aaggttagga aagaagcgcg gaatttcatc aggcaagtac ggcctttatt
    22501 ttggtccgct ctgaattggt cagtttcaat ccgctggctt ccattcccaa taaaaccgcg
    22561 ccgatgacgg gtttcacttc tgcaggaatt aattttgccc caggtgcaag agcgtggaca
    22621 gtttcacgca tgggggtaat gagggcttcg ccgcttttga acatggagcc gatgagcgca
    22681 acttcgaatt cgagggactc aaattcaagt tggcggatga ctgcgttggc gagctcgccg
    22741 agttcaatgc ctgcccaatg caccagcttt tttgcggttt catctccgct ttcggcggtt
    22801 tgaaagacga gcggggcggc atcggcgccg atggggtaat agccgttggt gtaaccttca
    22861 atgaggtctt cgaggttttt ggcgttgaag tgtttgatga aggcgtcgct gagcagtgtg
    22921 ggggggaggc gttttgtcca cgagtagccg atgatctgca tggtgcggta aatgagttca
    22981 ctggcgcctg cgccttcgcc catgagtgtt ccatagcctg aaacacggcc ttcacgtttg
    23041 tggtcacggt cccagccgcg gcaattgcag cctgtgccag aaacgacggc cacgccccag
    23101 ccttcgcttg tgcctgccag cagcccaagt gtgacatcgt tgaccatttt gaagggggcc
    23161 gtgatgccaa cattggtgat ggcgctgcgc atggcggttt cttcggcggg ccaatcgtag
    23221 ccgccgattc caaaccctgc cccggcaatc gcatccatgc ggatgttggc agtgttgagt
    23281 gcatcgttca atgcttcgcg aatggctttt tccatgccgt caaagccgac ggtctcatgg
    23341 ttgcctggtc cgccctgacc tgcgccaagc ggttctccat ttgcgttggt gatgagtgcg
    23401 gctgttttgg ttgcgccgat gtcaattcca agaaagtaag tcattcagtc agtgtcggtt
    23461 atttgaaaaa ttgaggcagg tgttcacggt gggtttcgag catgtcgtcg agcacagcct
    23521 gaaccttgtc ggctttcggg ccgatgggat gaacaagcaa agcctggtag gctgcatcgc
    23581 ggttaccatg aacggcggct tccacggtca gcaattcgta ggctttgacc gctgaaatca
    23641 acccaaagca ggaggtggga agtggttcag ccgcaatggg agtgataccg ctctttttga
    23701 ccgtggaggg catttcgagc acccattctg cgggccattc tttcactgcg ccattgttct
    23761 tgatgttgac cacgtgggtt tcacccaggt cgttgtaatg ggcgttgagc agctgggtgg
    23821 ctacggttga gtaataggcg ccgccgcgtt tcatcagatc ggcaggtggt tcgttgagtg
    23881 cagggtcggc gtattggcgc aacagatcct tctccacttc gatcacttct tcagcgcgtg
    23941 agggcggcca ggcttcctgt gccttgagct ttttatcggt gtgatagaaa tactgcaaat
    24001 aatagttggg gatcatgcgc agcacttcca cggtgcgcgg ttcccattca ggatgttctt
    24061 cctttttgag ttcgtcaatg tatccctgaa tcacctgcgg ccacacgtct tccccatcga
    24121 ctgtaaaccc gcgatgccat gaaagatgat tcaatccgag cgtcttcaat tcggcacgct
    24181 caggctcgat ctttctaccc gtggcctgct ccagcccttc gaggatgctc atcttcgctg
    24241 taatagggac attgcataca ccaaccgagg gcgtatcctt ggcatacatg ctcaatgcct
    24301 gggtgaccaa ccctgcagga ttggtgaagt tcaccagcat tgcgcccggc ttggaaagtt
    24361 cctgcatgtc tttggcaatt ttgaggatga cgggaatggt acgcaacgcc ttgcccatac
    24421 cgccaacacc tgtggtttcc tgtccgatca atccatggcg caggccaagg tattcatcgc
    24481 gtttgcgtgc ttccatgtgg cccacccgca attgggtggt gacgtaatcc gcatctttca
    24541 ctgcggcgcg ctggtcggtg gtgaggacga ccttgaaggg tgatcccttg gcttcgacca
    24601 tgcgttgggc aaatccgccg acgatatcga ggcgttcctt gtcgatatcc atcagccaga
    24661 gttcgttgac ggggaatttt tcgaggcggg cgaggaatcc attgatgagt tcgggcgtgt
    24721 acgtggaacc gccgccgatc actgcgactt ttaacataga tatatccttg gtttgttgga
    24781 gatggaaaac tgacgattac ttaaaaagcg aaggacactg ctggcacacg aaagggagct
    24841 tttcatcctc ttttcccttt tcagcgccca ttgtgtcctt cgcttttgtt tggtgtatta
    24901 attatttctc tacgcctgtc accacaatac cctgtacgaa gaaacgctgg gcgaccgcaa
    24961 acagaatcag cggcaggata agcgccatca atgcacccgc ctgaatcaat tgaggctttt
    25021 ggccatagat gccgttgaag gtggaaagcg caacagagat gggccagtta tcgcggttgg
    25081 tggaaagata gatcagcggg ccgaaataat cgttccatgc gaagacgatg tggaacactg
    25141 tcactgccat caatgcaggg taggattgcg ggatgatcac cgaccaaaga acacgcaggg
    25201 ggctggcacc gtcgatcatg gcggcctcgt ccaattcacg cggcagggtc atgaaatatt
    25261 ggcgcagcag gaagacgtcg taggcgtttg cgaaaaacga tggaacaatg agcggtaacc
    25321 atgtgccaac ccaaccgatc ttggcaaaga acgtgtaggt ggggatgacg gttacgaaag
    25381 cgggcaggaa gatggttgcg atcaggatga cgaataaaaa gtcacggccg gggaagcgga
    25441 aacgtgaaaa accgaatgca accagcgtgc aggagaacag aaccccgatc tctgtcatga
    25501 tggcataaaa ggtggtgttc caaagcaggc gcgggaagtc aatgttgttc caaacttcgg
    25561 catagttttc ccaggtggga gagaatgtcc acgggcggtc gagggtgcgc catgatccct
    25621 gccagacgat ttcgctgcca gttgggtttt gcgggtcgat aaaggtactt tccttttgac
    25681 cctttttcag catggcaagt tcgcgggtcg tcccatcagg aaagggaacc acaaaaacat
    25741 ccagtttgtc gccgttatat tcgaactttg gcggggcggc cgggtagaaa ggcgcaccga
    25801 cgatggagat ctggtcctgg gtcttgagcg aggtcaacac catgaaggca aatggggaga
    25861 ggtaaatgaa gaggatggca aaggtgaaag ccgttactgt gaaaatgctt attcttttct
    25921 gaagggggga aagtggtttg ctgtgcattt gtatctctcc ttaagaacgg ctgtcgcctg
    25981 cgtaatacac ccagtatttc gctgtgccaa acagaataaa agtgatcaat aggatgacga
    26041 taaagaggaa ccaggccagt gttgacccgt aggacatatc ctggaaggcg aaaaagtttt
    26101 tgaatagata aagactgtag aacatcgtcg caccgcctgg cctgcctgtc ccttgattga
    26161 caaccagcgg cacaagaaag tattggaaaa gaccaacgat attcaatgtg aggttataaa
    26221 agatgaccgg ggaaatcatt gggaaggtga ctttccagaa agtctgccat ccatttgcgc
    26281 catccacttt ggcggcatca taaagttcag tgggtacgcc ctgtaaaccc gccagcatga
    26341 tcaacatggc atttccgaca ccccatatac ccatgatcac ataggtagga tatacccagg
    26401 tggtgctatt ggcccaatca ggggtgacct ccttgggaac gcccaaaccg atcagggcgc
    26461 gattgatcca gcctgtttca gagttgagca taccgcccca gaggaaaact gaagcaacaa
    26521 agggaacgat aaaaggcatg taaaaaaggc tgcggaataa catttggcct ttcaggtttg
    26581 gattgttcat aagaacagcc agtcctaaag gcaccagaat tcctacaggc aaagcgatca
    26641 acccgaaact gatggtgaca cccattgaac tgggagttcc acctgcaagc caggtgatcc
    26701 aggtccgtgg gtcaacgcct gcctgacggt cgttaaacaa ctgaatatag tttgccaacc
    26761 ccacaaattt cggcgtattt aagatgccgt ctgtaatgtt gaggtcgaga aaactgaaga
    26821 tcagtgatgc gataatcggg aggaatgtga aggcgaagaa gccgattaac cagggggaaa
    26881 gaaaagccat tccctgcttt agttcccgtc gcgctaatgg agatgcattt cgaaatgact
    26941 ttatggaagg aatgtatttt ctgaaattgg caagttcttc aaacatagtc tctcccgggg
    27001 ttattatgtt ggcgggccaa cagagaagtt ggcccgccaa aagtatatca tgcaggatta
    27061 tttgttatag ataacgttga ggtcatctac gagcttctgg aattcagcat caaaatcgag
    27121 gccttcggtg ttgttcatca agtccatgaa gacttgctgg cgatcaaagg cttccttcca
    27181 attcggctgg tactgttcgg cgctgggggt gtcaggatag gcgagaccct gattgaagac
    27241 atcccaactt tccttggtca cgaacggata gtcagcagct ttcttgtcga agaatgcctg
    27301 ggtcttggat gcaatagcgg gcatggctcc ataggcgggc aggagtttgt caccaccggt
    27361 ggtgatcagg taagccaaaa cctggaaagc ttccttcggg tgtttggtgc ccttccaaat
    27421 gcggaaggtg tcggcatcaa tacggccatg gactacacca tcggaaccca tgggctgaat
    27481 accaagctgg aattcattgc cggctttggc gaaatcacca agacagcagg tgtaccaggt
    27541 ctgggtcaga cccatggcag ccttgccact gttaaagacg ttgccggtgc cgaattcggg
    27601 ggagttggcc agaggaccag tggggatgaa gggctgatcg ccatacatgc catcatagta
    27661 ccaatgccag gcttccttcc aaccatccgg aagagcggaa gcgtattcgc ccttcttttc
    27721 gccagaatag atcttggctg cgccggaata gaaggtggcg acggagttcg ggtgctgcca
    27781 ctgagcgcta taaccaacct gaacgatctg ggtgcggtca aaaccttctt cagttgcatt
    27841 cacaccattg acgtcaatgg tgaggcgttt ggcaacttcg gtaacggttt cccagttcca
    27901 gtcaacttct tttccatcga gggtgtattt ttcaccatac ttctggggcg ggtaagccaa
    27961 tccggcttca tcaaacatag agggtacata gtacattgca ccagggaata cggcgaacgg
    28021 caaaccaacc tgaccttctt cagtctgata gaacttcaag agcgcaggat caaagacgga
    28081 ggtgtcgaat ttggtttctt cgatgtaggg agcaatatcc agccactggc catagaaagc
    28141 attggaacca ccccagccga ccgggccgac gatatcgggg ccttcgcctg aagcgatctg
    28201 ggtggagagg gtatcacgag cgctgtcgaa aggaacgatc tggagtacga gttcgatatc
    28261 gctttgtgat gcattaaagt cttttacaac ttcttcttga accgtcacct gggcggggtc
    28321 ggtaccagta ccaagaccaa caaaccaacg aatctttact ttttcagcag gagcagatcc
    28381 gccgcaagcc gtgagaacca tcgaagcgac gatcatcagg ctaaatagtg caagtaaatg
    28441 ctttttcatg gtgctatttt ctcctctagt agaatttgaa atgacgattt atggaatata
    28501 gggttggtgg ctatttgtgt aggttgttcc tgcctccttc ttaaggattc acttcatgcg
    28561 aacaaccgca tgagtgccga atgatcaact ccgtgggtaa aagcgtgact ttttcaggga
    28621 gttgtttatc gaatatggaa aataactgtt ccgctgcgat ccgtccaact gattctgtag
    28681 gggctgaaac agtcgtcaat gggggggtga ggtatgcgga gatctttaca tcatcgaaac
    28741 ctgccactga aatatcttca gggacacgga aaccatgttc tttgagtgaa gcaatcaccc
    28801 caacggcggc atcatcatcg cctgcgaata cagaatcaaa agcgacgcgc tttccatttc
    28861 ccaaaaactg atttagggat ctgtaggcaa cgtcgcgttc aaaatcgcct tgaagcacaa
    28921 ggctatcatc aaaggggatt ccattcgctt ccagagcgga tttgtaaccc agttcgcgcc
    28981 aataagagtc ttcttgatga ataggaccac gcataaggag gattcgtcgt tttttgtgtt
    29041 cgtgaataag atgttccatc aggtgaaagg tggcggcttt attttccacg gtcaccgatg
    29101 ggatattgac agatggggga gaggagcggt gaatcagcac aaccggatac tcactattgt
    29161 acaggtcgat caatacttta tctgaaaggc tgtctgcaaa cacgagcaaa ccatcggtat
    29221 tatgggaacc aactggaggg gagaccacgg tttcttcatc tgctcggttg gtggctacga
    29281 tcaagttgta tttgtgctcc cgcacaacag attcaatgcc gctcagtaga ggtgcaaaaa
    29341 agtcattgtg catattggtc agcaaaaggc ctatgacgcg tgttttttta gaggcgagat
    29401 gacgtgctgc cgcatgggga acatatcgca gctcagccat gacctttttc aggcggtctg
    29461 aaacttccct tgaaatgggc gcattgtggt tgatatagcg gctaacggtt gcaaccgaaa
    29521 cacctgcttt ttcagccact tgcctgatag taacggagtc agattttcta gtcatagctt
    29581 cgtataaccg tatgtaacca gttacagtaa ccggttacat tatttcatac ttgcaaagct
    29641 aggtcaaggc acaaattctt aaaaaatcga ggtgtttttg gctctcaaat atagaatggt
    29701 atgaagacaa aaaagtggca atttagattt ttgatatcca aagttgccac ttttttgtta
    29761 cttgagtctg cagataatta ccccacccac atgtcataac taaataacag cacggacata
    29821 cccattaaca agatgcttat caaagtccat gctttatcaa cgaccttgtt tttaccccaa
    29881 taccccaaag taatgaaaat ggaaggtgcg atcaacatat atcttgccat gctttgtgct
    29941 gatccgctaa acacagaaag caccaccaca gccagactga ataaagccac aatgggatca
    30001 cggcgaatta accaaatgct gcagatcaac cccagcaaga ttgtaaatac ttcaatacca
    30061 aaatagatca acccctcctg actcacatac cttgcataga aataagcatc cttccaactt
    30121 tgaagcgaac tctcaatcgt catcagaccg cggccaaaat aaaatgcctg taattcttcc
    30181 cagcctttgc ccagtgggga agtgcgccag atgaagtagc ctatcaaagg caggaaggcg
    30241 agcaatcctt gcagaaacca cttccaggtt aattgcgatc tcaggttcga tttccaatct
    30301 gtattgataa tccatgccaa caacaaaggc agagccatgg ccgctccgtg tgcgcgcgtc
    30361 catgcagcca gaaatcccaa tatagatgcc caaacccatt gctttctttt ggtcagcatc
    30421 agcgccgaaa aagccagccc aacgaacaat ccctctgtat aaatttgtgc aaagaaaaat
    30481 gcagaaggaa atacaagcat gtagaacaca gcccgccagg cgctgtcttc atcaaagagg
    30541 ccttgagtaa gatcccagac ggcgaacaat ccagccaatg ttccaagcaa ggtgatgatg
    30601 cttcccgcca aagtggcagt gccaattgca gaaaggccaa atatcctcaa tggaaacgcc
    30661 actgcactca taagatatgg atacaacgga aagaacgaat aattcttcgc aatcggaagc
    30721 ccggtggcag ggtttcgtac ataccccgct tcagggtcat cgtacccgcc gaccgcaata
    30781 cccacataat actcagaatc ccatgccacc tgccgattca tgaaaggctc cagtaaatat
    30841 atcttgccat cattgctgct tggcagggtt tcattggcac tccaaaccac agcctcatct
    30901 ggctgtttta attgcagacg ggttgtcacc agccattgaa atccgtacaa cagagtaaac
    30961 cacacagccc ataaaataac caagtttctt ttcatagtat tcctttattt gatgatgaat
    31021 agagtgatgg attgcgcagg gaaagaatgg ttccctgata gatcaacagt gccgatgttc
    31081 tctgcctgat gtacaggatc gaacaaccac gcttcgccct taacggctgc ctgaccagca
    31141 attcgaaggg attgaatctg ctcttcaagt gacaggttga ccacaagaac ggtcaatgca
    31201 ccatcatcac gaagggaggc atacaccgta aggtcagcga tatcagacga tgaatagacc
    31261 agctcgtcgc cgaatttttt atacatctga tacgtgaaat aacttggatt cacatcggtc
    31321 tgggcgatca acccccagcc gccgttccca cccttggacg tgagcatcca atgattggcc
    31381 atgaacacgc cattctttat catttgccca agcacatcac cgagccagat ggcattgtaa
    31441 tgtgaatcgg gtgtcgattc cccgcctact gacttgctgt agtctgaatt gaattctgtc
    31501 accgcgatcg ggatatcacg ccctgtttcc gtgtggatca actcccgcag gtgaatgatg
    31561 atcttgtccc actcccgcgc attgacacgc aattcctcga tcgacgcagg tcccgatgtg
    31621 aatgaacgtg gaaagggata gcgatgaaag gaaacgatat ctacaaggtc gccattgctt
    31681 ttcaggaact ctctcatcca aagttcattg gcttcactaa aattggtgct tgcgccgtta
    31741 tagtcaaaac taaattgatg cgtttcaggg ccaaccaatt ttatacttga gtctactttt
    31801 ttcatggcct tggcaaatgc ccgccattct tgattgaagc gctcaatatc gtaactttcc
    31861 cctctgttct ttaactcgcc atcatataaa gaaggctcgt tgccaatacc ccaatacacc
    31921 actccatact tttgttcctt gttcacatac cttaccattt ctgcagcctg ctcgggcgtt
    31981 ccacccagca ggcgtacatt gatcatggct gtagccccca ctttttcaat aaagctcatg
    32041 agttgatcga tctgatatgt ttgaacattg ttgtggtcgc cccatgaacc agcaggaaaa
    32101 cgcacaatgc tgacccccga atcgtacgcg ggctgcaacc cttccactga aagcgcgatc
    32161 cacggaccgt agttggtgcc gtacaccagt ggactgattt gcccaaggga gatgcctgga
    32221 tcaacaaaca atacattggg gtatggagtt ggcacaatgg ttgccgtggc aagcggggct
    32281 gggctttgcg cgcaggcagt aacaagaaac acaataacca gcaaagcggt aaagaggggg
    32341 ggcttcatag cgatgctcca atgtagattt tattttgata tgcaagagat caggtcacga
    32401 atgatacccg atgcctccac ctgggtcagt gccagcacag gaacatcacc cgcagaatac
    32461 acctcgcgat atgcctgccc cgaattcacg gagcctcgca aaatgaatcc cacactattg
    32521 gattcagact ccaccgcttc aagcatccgt tgagaatcga ctgccacctt tgcaaatgac
    32581 gtaacgattc tgccttccat cacggcctgc tcgaacaaaa cctgcatatc cgcatctgat
    32641 gcatagaccc agacctgcgt cgtggaattc tcctgcgcgg aaaataaact ttgcacctct
    32701 tcaagggtga ggtcttgcaa tgggctgtct tgatgaacga tcacaagcag ttcatccttt
    32761 ccgacctgat atgctggtga tacaaggggc tcaggttcgc ccacgcgcag aaggatatca
    32821 ggtgaatctg ctgaaatgtt aatggctgtg ttcaggctct cggcgcagga aaacaactcc
    32881 accagccagg gattggctgc agatgtggaa taggcagtaa cgatctgggg ttgagtggtg
    32941 gtctttgcag gtggggtaca ggatgcgagc aggaatatca atatgaggaa ctttttcatt
    33001 ttttgatttt actgcttccc ataccgaact caccagtttt cgatcagcac ggacagtaac
    33061 tttgcccgtt cttcgaggct atcagcaaaa atgtattcgc gttcggagtg ataaccttca
    33121 ccgatcgcgc ccattccatc caacacgggg atgcccaatg gggcgacgaa atttccatct
    33181 gaggcgccgc ctgtgccgcc cgctttcaat tccagcccca acgtttcctt tgcgatctgg
    33241 gctgcctttt tgaaagccgc ctgattgatt tcgttaaacg gcagcggcgg acggttcaat
    33301 tctcccgata cctcaatgga ggttccatcg agtacgggtt ttagtttttt catttcccct
    33361 tcgagccttt cccattctcc gggctgcatt acgcgcacat ccactacaat gctggcttct
    33421 tcgggcacca catttgagac cgtaccgccg tgaatgatgc ccacgttgag ggtggtttgt
    33481 ttttcataat cagtcaattt ttgaatggcg atgacctgat gtgccatttc ttcgattgca
    33541 ttgcggcctt ccttgtgatc tccacctgca tgagcggcac gccctttgac cttgaccctg
    33601 aattccccca cccctttgcg ccatgttttg agcgaaccat ccatcagcgc accttccatt
    33661 acgaagacga tggaggcttc actcgccagc ttttctatgt attcacgcga agtaaaactt
    33721 ccgatttctt cgtcagaggt gaacagggct gtgatgggat gaggaacagc cgtccctttg
    33781 gaaagaagcg cggagatccc gccgagggcg atgacaatgc ctgctttcat atccaatgta
    33841 ccggggccgt gaatcttccc atccacctct ttgtaggtga aggtttgtgt agtgcctttt
    33901 ggaaagacgg tatccatatg gcagaggagc agagggccgc ctttgccggt accgccccat
    33961 ctggcgagga catggtcgcc tgtttgttca ttgggcagga tttcaacctg cgcgttgagt
    34021 ttgcggcatt cttcagcgac gatctttccg acgcgatcca ctgcggcttt gtcgtgagag
    34081 ggtgattcgg tttcgacgag tagtttgagt aggtttttca tcatgggagt ttttgaataa
    34141 ataggacggc gatgcctgca accccgcaat atgcagagaa gacgtagagg gagtgtttgt
    34201 tgttgtggag ataactcatc agccatttga tggcaagcca gccgacggcg gcggatgtga
    34261 ggaagcctgc cagcaaatag gggaggaagg cgcgggttcc gcttctttcg atcacgctga
    34321 gcatttcata ggagcctgcc gcaagcatga ctgggatgga catgaggaat gcaaatcggg
    34381 cggaatcttc gcgggtaaat ccgcggacca tgctgccagc aatggtggtg cccgaacggg
    34441 acgccccagg gaaaacagca atgatctgga acaaaccaac aatgagggcg tcgagccagg
    34501 tcatggtttc gaggccgcgt gtttttttat caaggaattc ggccattgcc agcaggaaag
    34561 atgctgtgaa taggcggatg gcggcttcga tcaaaggcgt tttgaacaag gcttgtacag
    34621 agtccctcaa gaaaaacccc gctatcagcg cgggaatggt tgcgatgaca atgtaccagc
    34681 caagttttgc attttgcggc atttgttcaa agtttttcag attgccaaga tttttgagcg
    34741 tatcaagggc gatcgacaac aggtctttcc agaagaaaaa gaacagagag accacggttc
    34801 ccatctgtac aagaacgctg aaggaaaatg ctgcatctgt tgcagggatt ttgagcaggt
    34861 tttgggcaac cagcaggtgg gctgtggagg agactgggat gaattcggtt aatccctgga
    34921 cgattccaag gatgaacacg tcgaagagag acattggttt tccttaattg tttgatattt
    34981 gggctaagtg gttagctcaa atgaatttac ggtctattct ggcgggtaaa gatttgtgta
    35041 tttatgcctc ccccacactg tgtttgtgca atgtggacga ggcataaata ttgcagattc
    35101 aaagttgggc ctggacttta cccataaaat gtcaaaattt cagacttgca tgtgtttaca
    35161 tttacgagtg actgagcttt gttctggctg gagtttactc tcttatttcg atgtgcctga
    35221 cttgccaatt aattggcgaa gatcccataa tacagcgatg gagaggaaga tgaaaagaaa
    35281 ttcgtaattg ctttctttta cttcctgcac agcctgaaca aaggaaatgg tttgatacga
    35341 atagaaagag cggaagatgg gttttgcaac ctgagcggcc aggtaattaa aaatgaacag
    35401 caagccaatg tcccaatgga cgatcggggt gaatttttcg gcgaaagctt tgaatggttt
    35461 ggaggccagg ctcagagcag ggagcatcgc cgcgaagccg acccagaaaa cggtgaagat
    35521 gttatcggct ttcagaatgt cgctgttttc gaagaccgca aggttgtgga cgtttaattc
    35581 ctcctggaca ttttcgatgg gcaggttttc tggttcaggg atgttgaata tcctttgtcc
    35641 ccagctgatc tcttcgcctg cgccaaagaa gtaaagcatg gccagaccga gatagaccag
    35701 taacttaaac cagtgaatat tggcggtggt gcgggccttc aatgcctgtt ggaaaatata
    35761 gaatgatatg accgaggcga tgcccaacga tagtgccccg acattttcaa agtaagtgtc
    35821 ttcgagaaag agcttgtcac cccatttctc tccgagggcc agagtgcaaa agcccaaaat
    35881 gaaatagata acgaccacaa cataaaagat gggacggaag aacgattttt ctttgctcat
    35941 tttctgattc cttttttctg gaatttgttt ctttgtttgg agggttggat gtcttattct
    36001 gggatcgaca tcttggcacc cgttaattat acaaacgtca tgataccttg agttgaccca
    36061 aataggattg cctttgcatt tcaaggcctt gtgtttttac gcgccgctcc ctgcccgttt
    36121 ggcattattc gaccattgat cgagcaattc aggcgcacgt gattcgaacg tgccttcggc
    36181 aatgtaaagc ctgatctttt ccatcaagcg gatcagggcg cgcaggttat ggatggagat
    36241 caacgtgcca gcaagcagtt cctttgccac aatgaggtga cggatatagg cgcgagtgaa
    36301 ggtcttgcag gcatagcaat cgcaggtttc atcaatgggg cgttcatctc ttgcaaaggt
    36361 tgcgttcatc aggttgagcc gcccttcggg cgcaaaggcc gaatgatgac gcgccaggcg
    36421 ggtgggtaac acacagtcga aaacatccac gccgcgtaaa acgccattga taaggtcttc
    36481 gggagtgcca acgcccatca ggtagcgcgg tttgttttca ggcagcaatg gcatcactac
    36541 gtcaagcgtg tcgtgcattt cctgtttggt ctcgcccacc gaaagtccgc caatggcgat
    36601 cccaggtgta ttgagagaag cgataaattg cgctgactca gcccgcaagt ctgcattgac
    36661 gccgccttgc acgataccga acaaagcctg atcggggcgg gtctttgcct tgagggaacg
    36721 ttctgcccag cgatgggtgc ggtccatggc gatcttgctg taggcaaggt cattggggtc
    36781 ggagcattca tcgaaagcca tgatgatatc tgcgccgagg ttttcctgaa tggcgatcga
    36841 tttttcgggt gtaaagcgat gtgtggagcc atcaatatga ctcttgaagg ttacaccgtc
    36901 atcgtctatt ttgcgggtgt tcgagagtga gaaaacctga aagccgcccg agtcggtgag
    36961 catggggttg ggccattgca taaatttatg cagtccgccc atatcgcgca caagttcgtc
    37021 gccggggcgc agatacaaat gataagtatt gctcaaaacc agcgaggctt gaatatcctt
    37081 gaggtgttcg ggggtgagcg tcttgaccgt agcctgcgtg ccaacagggg caaaaacggg
    37141 tgtaacaagg tctccgtgtg gggtggagaa aatgccggtg cgggcgcggt tgttttttga
    37201 tgtgatttga aaagagaaca tgcagcgatt ataagcgatg aaaatgacag ctatgccgtg
    37261 gcgaacaggc acttgaggta tgcgccttct ataaagcgga tcgggtggtc gagggcgtgc
    37321 cccgtgcgct cgatttcgat taagctgcgc cccgcttcct gtgcggacct gtgaatggat
    37381 tcgaaaaacg aatcagaatc tacgcggctg gagcaggaag cctgcaccaa catgccgccc
    37441 cgccgcaaaa cgcccagccc aagctgggtc agtttgcgat aggctgataa tgctccagcg
    37501 gtttgtttct cactttgggc aaacatgggc gggtcaatga tgaccacatc gaaaaggcgt
    37561 ttttgcgatt gcatccgtgc aaggacttca aaggcatctt cggcgaaggt ttcgtgttgg
    37621 gcagaggcca cagtggggaa gtgctgattg tgtttcatgt tgcggatggc cgcttcaatg
    37681 gcgggcgggc tgatatccac actggtcact tctttggcac cgccccgcgc agcatagacg
    37741 gaaaacccgc ctgtgtaggc gaatacattt aacacagttt tatttttgga aaatttctcc
    37801 acccgtgtac ggttttcgcg ctgatcgagg aagaaacctg tcttctgccc atgaatgggg
    37861 tcagattcaa acgtgaggcc attctcttga aacaggatca ggcttgggga agtctgatcc
    37921 gtgatcgtca tcccatcgtt gaggtggtgt agaaattccg tctgcttttg aagagaacgg
    37981 ttgaggcgca gaatcaaatg actggccgtg ttgacttgtg ccaatgcgtc gcaaaaactt
    38041 ttgaggtgcg ggatccatgc gggggtgtaa agttttagga cgagcgtatc ggcgtaacga
    38101 tcgatcacca gcccgggcag gccgtcgttt tcaccgtgga cgagccgata gcctgtcgtt
    38161 ttttcttttt ccaggtgggc gcgggtttgg gcggcgattt tgagtttgtt ttgaaaccac
    38221 tctgtgttga tggttgcagg atcgcgcgat tgcaaaatgc gcacgcggat cggcgaggtg
    38281 gggtcgtata acccgatggc aagaaagcgg cgtttgtcgt cgaagatcac agcgaggtcg
    38341 ccaggtgcgc cctggtggct ttgctcggtg atcgattgat cgaatatcca cggatgcccc
    38401 tgccgcaacg cgcgttctgc gggcgggctg atgcgcagcg cgatccgctt tgcagacggg
    38461 ttggggagtt gcgagaggga attgttgaat tccatgatgc tggcattata cagttcaatc
    38521 ccctctgctg tatggaatgt gctatccaaa tttttcgtgc tcttccatat ccattaataa
    38581 cagatccaga gcgccgacag aaacctgcag ttcacgcagc gagatcaaca tggagatgat
    38641 catcaaaatt agagcgatgc cgaaactcca attgccgcct agggtccagc ctgcaaacag
    38701 ggcgaacatg ctgaccacgc agaacagcag gctgagcacg ccgacgatct gcatattgcg
    38761 gatgaggtaa acgcgatatc gcaggttcgc gatctgaccg agcaggtttt cattgtggtc
    38821 tgttttatat ttttcgtgca ggttgcggat gatgcttgcg agggttaaaa atcggttggt
    38881 gtaggccagc atcaacaagg atacggttgg gaaaagcagg gcaggggtgg taagggtaac
    38941 gtccatgctg ggaattatat ctctatagaa tttcgtggga gtggctaagt ctttggggta
    39001 aacacaaaga cgccaagcca caaaaccac
//
LOCUS       DAOQLC010000024        39011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_299_length_39011_cov_24.918344, whole genome shotgun sequence.
ACCESSION   DAOQLC010000024 DAOQLC010000000
VERSION     DAOQLC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 39011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_299_length_39011_cov_24.918344"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1807)
                     /locus_tag="PL000_06200"
     CDS             complement(137..1807)
                     /locus_tag="PL000_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88522.1"
                     /translation="MNTLKLNRRPLILPLWSGLIGILPLTFLFDPPRASLKSLLELSF
                     VNLFTVGVLVFFIWFYFVRIKKELKYRIVFTSEAIEENQNKGFYSSRPRRILYSDVLS
                     VQYLFPGVIVVRSKNEEDFKFVLADIEGGADRVMELLKTHLPGTVIDEKLPEKMEKNS
                     ILVKINDAGMVLFLPLITIPVLGVYGFFQGGWNVHRLPWNEFLLDFSITSKNSPWIAS
                     TDFNGDNLIVHFDDQEQRWPLPLQNPRGTIILSDSNGQPTLMTGEINYTWQDGKWVQW
                     KYFGVYKAAIYRKRFVSTTEQAWLVLYGDNKPNVLVGATAGRPGVDVIQLPESAVQKG
                     YEPYSLSILADETVLVHAGNENEARIFLLKDGQWLEKSYDVPFDFLQVKDFIVDDVGR
                     LFIFYTTQTEEVESYFVQIQDDEKALITEIPLLGEFSDYDSLLVDSHDRLWILDDSYG
                     GGVTVLRPVWSGVAELVVQYNKENSNYPRSGRSSFVLGADGKVWVSSEDLYWIDSTAS
                     ELPRPMPDWLMDIGYSDTGYLKYIAILAGVFLGGYGILYLLSTKELKK"
     gene            complement(1865..2224)
                     /locus_tag="PL000_06205"
     CDS             complement(1865..2224)
                     /locus_tag="PL000_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC88523.1"
                     /translation="MAVDLTQFEKEQYCYLTTRGRKTGDPHEIEIWFCVHEGALYLLS
                     GNMDRSDWVRNLLKDPNVTVRIAGQTFPAVAKLLDLKDGQTETLVRMKMAIKYNEWEG
                     RSPSEWARTALVVRVEV"
     gene            complement(2227..2784)
                     /locus_tag="PL000_06210"
     CDS             complement(2227..2784)
                     /locus_tag="PL000_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88524.1"
                     /translation="MTNSSRISSGFPPFQQGGLDSLCGLYSIVNAERFINHSSDEETQ
                     QLFDDLIHYLSRRGLLGKFLIGGILHHQMLLILNKVVGKKRISNVQIPWRGVPNPDLT
                     TFWHSMQAFLDGTPGRSIILGLEGYHDHWTVIEKITSRSILLYDSSLIKRLPRGRCTT
                     VYATEKRVHILLPAQTYFLSNEKEA"
     gene            complement(2806..3960)
                     /locus_tag="PL000_06215"
     CDS             complement(2806..3960)
                     /locus_tag="PL000_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88525.1"
                     /translation="MTSFLTDISSEMLVYLIPLFLSNVLGVRMAFIGLIDGIAETTAS
                     LLKVYSGALSDKLGQRKGLAVLGYAISTFAKPFLYFANSWGWVLGVRFSDRVGKGIRT
                     APRDALVAASTDETNRGLAFGLHRAGDTAGAFLGIAIAALIVWLTQQGALQITREAFQ
                     TAVLASIIPAVLAVIVLGVGAADVAASGKGAEKKRLSFKEIDPRFGKFLLITILFTLG
                     NSSDSFIILRAQERGLNVLQTLFMLMTFTAIYTILSGPLGALSDKVGRRRLIIGGWMA
                     YGLVYLGFAFAGAGWQIWALFGLYGIYYAATEGVAKAFVADLVPEAQRGAAYGWFNAA
                     IGITALPASLLAGLLWQGLGSWAGFGASAPFFFGAALALAAGILLWRLVE"
     gene            complement(4087..4539)
                     /locus_tag="PL000_06220"
     CDS             complement(4087..4539)
                     /locus_tag="PL000_06220"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HNC88526.1"
                     /translation="MKTLLEKSKYLAIVGVLSLLIAAVAAFAWGTLKTAAAVLLVFES
                     MGKDAAITIEFIEIVDSFLIATAILIFAVSLYELFISPLELPEWMLAHNLYELKAKLS
                     SMIVLVMGVKFLQKLLEVKDTNDLLRTGVATALMSAVLIGFGYFGKKD"
     gene            complement(4550..5380)
                     /locus_tag="PL000_06225"
     CDS             complement(4550..5380)
                     /locus_tag="PL000_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC88527.1"
                     /translation="MHRERVSENVFWFQSEVYAQVTAGVIVGPQWAVVIDTLALPEET
                     LGMREFIEHELGVQVRYVINTHYHADHTWGNCFFPGATVIGHAKCRELLIEHGIPSLE
                     GARKQNPNLRQVKIIPPHLTFDSGELTMRVGKKNLVFSQSFGHSEDGISVLVEEDRVL
                     FAGDAFMSLPYIVDGDIDEMISSVKRIGRMGLENIIQGHGDIILRGEIDAAVKENMNY
                     LNNIKKLVKAAGKRKNADEYLDAITIEECGKSRVYLGGLAETLHRRNLRALYRQMYGE
                     "
     gene            5536..7239
                     /locus_tag="PL000_06230"
     CDS             5536..7239
                     /locus_tag="PL000_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC88528.1"
                     /translation="MPADILVVTSSSQLGESIRRILEETDLYRIHVVTNKSSAIVRAD
                     EVGAPLAMLDLALGEGVIREIGQSLRTIRPGINLIILCDENITPPPFDELRPWILVRK
                     PFKMSNFMTAISQPQTTTATPPTPATTTSTPAAAPQNTSTPTLPWLSDPNRAAQHLTR
                     LTLESSAQAALITRKSDLWAYAGSLSQGAAKEVAQTITRNWDGQKGFDLLRFIRLEST
                     KAEHMLYATRLAADTVLALVFDAETPFSTIRAQASQLVTNLDNETAEPQKPSQPFNAE
                     KQVVEADADDGDAPNIPLISDILNNVPTPNPEPATTRDFNLPRKKETQEAFDPNQTRI
                     SESLSNASVFKRDPSPAVHYGSIPVLNKKPAPSIPEVQVQPQNQAVLNEVDVTAPSRI
                     QQRPETPVAKPAPGEQEVTRESPTTEAARKLVVEPTTAGLYHLTYACLLVPRFSAHYL
                     TGDLADQLGEWLPNICIAFGWRLEFLSVRPEYLQWVVNVQPNTSPGYLMRIMRQQTSE
                     KIFTDFTRLKRENPSGDFWAPGYLIMGGTQPHPQQLVRDYIRQTRQRQGQELPSEPKR
                     K"
     gene            7421..10213
                     /gene="polA"
                     /locus_tag="PL000_06235"
     CDS             7421..10213
                     /gene="polA"
                     /locus_tag="PL000_06235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNC88529.1"
                     /translation="MPPTLYLIDGHALAYRMYFALTAGGGSQRWQNSKGEPTAGIYGF
                     ARELVRVLEQEKPEYLAVAFDVGKTFRDDIFPEYKGTREKMPDDLRPQIERIRQMVDM
                     FNIPRLEMDGYEADDVLGSAARIAAKKGLGVKIITGDRDLLQLVNERTAVYLAGDDQT
                     YITDADVIKKLGVRPTQVVDYKAIVGDTSDNIPGVKGVGEKTAIALLEKFGTLDAIYA
                     NLDQVENRWKGKFEASKDAAYMSYKLAKIEVDLKIDLNLDHAAVRPFDSSALAAFFQE
                     MEFKTLLNKLPAISGSAPSVEIAAAPKKGKAGEQMSMFVNAPVIVSTPSKIEVTVVDT
                     DEKLGALKNELTRAKVISFDTETTSTEEVTAELVGISLAVKEGHGYYIPVGHVSGNNL
                     EIKKVIAALTPAMTDSKIGKVAHNAKYDYIMLARYGLVISPLTFDTMLAEFIVDPSSR
                     NLGLKNLSEYRLGEEMTHIEELIGKGKKQISMAEVAIESVANYAAADAETTLRLMPIM
                     QKELQRVNGTKLLDEIDMPLTPVLAEMEMTGALIDTKFFAAMSQELGQRLAEIEKEIF
                     QLAGKTFNLNSPQQLSDVLFNHLRLEPPDKGRKTTTGFYSTSADVLEALRGKHPALDF
                     ILEHRELSKLKSTYVDALPAAMDPNTGRVHTSYSQIGAVTGRLSSNNPNLQNIPIRTE
                     TGRRVRNGFIADKGNVLLSVDYSQIELRIVAHMAQDEAMLKAFRAGEDIHATTAAAIY
                     DIELDNVTKDMRRHAKAINFGLIYGMSAFGLTRSTDLTLAEAEEFVKAYFTKFPGVKK
                     YLDGMRRQAAEIGYVETLLGRKRYFPALQGKINMQLKNREEREAINAPIQGTAADIMK
                     LAMLKIPSALQAAGLKGKMLLQVHDELVLECPKKEVEKTAKVVQETMAEAYPMSIPLE
                     TEARAGASWGEMTVLK"
     gene            10305..12620
                     /locus_tag="PL000_06240"
     CDS             10305..12620
                     /locus_tag="PL000_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC88530.1"
                     /translation="MPGREDVFQKAMNDGHSAAWDQEWDKACAAYRRALEEFPDHPKA
                     LNSLGLALYQLGNFEEALQIYRNVVRLSPDDPIPMEKVSQISERIGDLKTAVEAAMRA
                     GDLFLQQRDTEKALENWVRVTNVNPENAIAHSRLAQVHERLGHMQQAVTEYLAIASVI
                     QRAGNAEKTQEMVSKALSLLPDSPEAKQAQTSLKMGQLLPKPMRGKGGTGPIRMAMVK
                     QLQKPTVSQSASGLDPIAEARQKALTQLAELLFEFSDDNPSAQEKRGLSAIMKGTGGL
                     SLQQSEQTKAVLHLGLAIDAQTKGNETQAAEEMEGALEAGFKHASLYFTLGLLRFKGN
                     RLESAQRFLQNSVKHNDYALGTRLLLGQLLVKKSLYDEAAIEYLEALKLADSITVPAE
                     KADEVSQQYEPLIEAHQSQKDENVLRKVCENINGLLMRPDWREQLHKTREQMPRQEGE
                     MLAPLADVILEAQSSSVLESMNRINQLARIGSLRSAMDEAYHAVQYAPTYLPLHALMG
                     DLLVQENRDADAIAKYSIVAHLYGVRGEVNQATKLLRRIIQLAPMDLGSRNRLIDQLV
                     ARGHADEAVREYLELAAIHYRLAELDLARKTYTTALRLVQQGNANRDWNAHIMQRMAD
                     IDLQRLDWKQALRIYEQLRSITPDDEATRRQLVDLNLRMAQPDKAMSELENYITYLES
                     QGKNDLSITFIQDLLQEHPDQLLLKRVLAARLHFAGRTEEAISILDVLGENLMQTGDK
                     HGAMEVINQIILMNPPNVSDYRQLLIQMQGN"
     gene            12781..13809
                     /locus_tag="PL000_06245"
     CDS             12781..13809
                     /locus_tag="PL000_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HNC88531.1"
                     /translation="MLKTQNTAVMKWLYVFAFVVAFLVVFGGFVRLTRSGLSIVEWNP
                     ISGAMPPSGQQAWEEEFAKYQETPEYKLINTGMTLPEYKFIFYMEWFHRNVARLAGLF
                     YAFPVFYFLFKKTIPFKEFGIYFVMGILFISQAVAGWIMVASGLDKRPAVSHFNLTIH
                     LLLALTLFSLALWTAFGHKYGFHDTSKKAAWSLPSKLAVFSFVILAIQVSYGGFTAGL
                     KAGHVSDTWPLMLGKLIPPNLFNSWINIFESPQTIVFIHRWFAWLGLILVPAVYYLVK
                     KQNYPADIQNGLKWLIGVVALQITLGVLTILSYVNIVIALIHQANAIVLVGLAVFFIH
                     RFRAVDRK"
     gene            complement(13885..14079)
                     /locus_tag="PL000_06250"
     CDS             complement(13885..14079)
                     /locus_tag="PL000_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88532.1"
                     /translation="MRSPRFLLTLGLFLMILGIALPFMMVIHVIESTFLINFFSWASS
                     VVGLALGMIGATFMAARRRE"
     gene            complement(14076..15080)
                     /locus_tag="PL000_06255"
     CDS             complement(14076..15080)
                     /locus_tag="PL000_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88533.1"
                     /translation="MTVNFEEPKETLLSIRDLHVWYELRRFGFGHAGYVKAVDGVSFD
                     LAEGETVAVVGESGCGKSSLMKTILGLNIPTSGEVIFGGVNLAGLKGNLLRQYRFEVG
                     YVQQDPYGALPPFMTIQQILEEPLIISGVKDKTERLDRIRKAMDEVKMYPANDFLPKF
                     PHMLSGGQQQRIVIARAMILGPKLIVADEPVSMLDASVRVEILKLMRTLQEQHQLSVI
                     YITHDLSTVKYFSSRIFVMYAGNLIEKADTRRLLDNPLHPYTMALLAATSDPDARNAD
                     TYKEVPPGEPPSLVNPPKGCRFHPRCARAIAGLCDQKEPVDFEPEPEHFVACWLYQPQ
                     "
     gene            complement(15132..15713)
                     /locus_tag="PL000_06260"
     CDS             complement(15132..15713)
                     /locus_tag="PL000_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC88534.1"
                     /translation="MSPQALYDVLYMFKDIHKVVLDFASRLDDDQMHWRPRGYSTSIG
                     FHLWHLARESDYLKAIILERTPQLVPEFGTPLEIWVKEGLAQKWGFPTGLNATVGTGL
                     SDEAVETLPIPRKEELVDYLQRSYDAVEEFVERLDERYPNFDDVDDELKKKLQNIRLN
                     LLVFLTHDCRHLGMMECLKGLQTGFGSATENRK"
     gene            complement(15732..16694)
                     /locus_tag="PL000_06265"
     CDS             complement(15732..16694)
                     /locus_tag="PL000_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88535.1"
                     /translation="MNAAPEKLLHIKNLVLHFKTANGSVQAVDGVDFDLDSNRAVVIL
                     GESGCGKTSLSKALLRLLPRNVDKYSGHVYLQGTDVMALDNEEYRQNVRWVGMSLVPQ
                     AAMNSLNPVLRVGDQVAEPAMVHLGLSKVDAYKLVYKMFQHVGVPADFVERYPFELSG
                     GMRQRVALAMALVTSPSLIVLDEPTSALDLLTQANIMNVLKRIKHELGTSFILITHDI
                     ATSSELADEVAIMYAGQIVETGDAKDFFPAPLHPYSQMLMASVPRLRTDSDPMFITGQ
                     PPSLMNPPKGCRFAARCPYRFEKCSEEPPVFQKANRKVKCWLHA"
     gene            complement(16691..18028)
                     /locus_tag="PL000_06270"
     CDS             complement(16691..18028)
                     /locus_tag="PL000_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88536.1"
                     /translation="MNNFRSSFQKLVQYPSAVAGIFVVFALVFVAAYAMITIPYTEAV
                     RLWRGGEEVWYQNPRFAAPAWINYFSSKQYSESFAVNTKDGQMTKTVTPGNEGISTFD
                     MAYEFDYSYDVYPQDLILYVTTTYKEKQPFISVELLTPDERTIRIGNFSVSNEFTYRF
                     SQDEKLLTKLRADEVIPALFSLPGSDQPLKGKYKLIITGTTFEPDSDLNVEFVSHGQV
                     FGWAGTDHARRDLVLPLLWGVPVALAFGLIASMGTSILTMVIAAIGAWYAGWVDELIQ
                     RITEVNLVLPFYALLIMIGTFYSRSIWVILGATIILSIFTGSIKAYRAIFIQVKESMY
                     IEAAKAYGASSPRIIFLYLIPRMIPLLIPSLVQSVPAFVFLEASLAVIGLGDPVLPTW
                     GKIIQDANSNGALYKGYYYWVLEPAMLLMITGLAFAFLGFALDRVFNPKLRDV"
     gene            complement(18034..19152)
                     /locus_tag="PL000_06275"
     CDS             complement(18034..19152)
                     /locus_tag="PL000_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88537.1"
                     /translation="MSATAQPLENVVVKKSFLNSSFARILRYSAKRLLTLFITVVIGI
                     YLTILIANMGGFVDEIIKGQIRDSVTQRIANNPTALQMAPDVRKKLIEDQIALEEKRR
                     GLDTPIAVRNFRYLSNALQLDLGRAINLTSDSGSKQVRLILLERLPATLLLMGTSQLF
                     LFFSSIFIALSLSRKYGSFLDKLVIALSPTSAVPPWFYGIFLILIFAAALKVLPFGGM
                     VDSPPPDNTFDYSMSVLKHLTLPCLALILSSFFLSVYNYRTFFLIYSSEDYVDMAKAK
                     GLPTRDIERTYILRPTLPTIITNFSLLIITLWTGALFTETVFLWPGLGRTLFQAIGQY
                     DTPIIVGSSIIFAYMLAMTVFLLDFIYALVDPRVKIGG"
     gene            complement(19212..21800)
                     /locus_tag="PL000_06280"
     CDS             complement(19212..21800)
                     /locus_tag="PL000_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC88538.1"
                     /translation="MKLKKLYALFSLLVVFSMILAACAPAATEAVEEPAPTDAPAATE
                     PPTTAATEAPAAPATDRRGGWLDEIDFSVVAADSAISQLQAGTIDFYSFALASDAFPA
                     IKEAGLGYTQSFGGSYAISMNPAVFTDTAVLNPFSNRKIREAMNWLIDRNYINQEIYG
                     GGSLPKLFSITTQLVEYTNLVETARALETKYAYNLDQAKEVISAEMVGMGAELGADGK
                     WQYNGAPITLAFLIRSDGDGTRQPMGDYVSNQLEAIGFTVDRQYKTSSEAFPIWQGTD
                     AKDGQWNLYTAGYLPSGLTRDERANFQQYYLNTSVQAAEPFLSNVSDPEFQKLGDDLA
                     LGNFSTKEERDAMMAEALRLSLEDSLFVFVIDQQTYAPFNSNVSVTYDLAAGYESTFM
                     GNYNLRFKDQEGGTMKIGTNDLFTQPWNTIGGSNWVWDSNVMRATTQGADSITGTGGI
                     MGDPYTGLAWPQRIESASLTYQKGLPITHNLDWLSVEAVDQIDVPEDAWVDWDAANQK
                     FITAGEKFPDGTTAKVKSVVTFPADLYDTVKWHDGSSISVADFVMSIIIFFDRAKPES
                     YVYDEAAVPGLAGFLPSFKGYRIVSTDPLTIEAYSDSYNADAELNILTLWPNSPFGLS
                     GENSWQILAVSNLAEAAGELAYSSDKADVAQIEQMSWVGGPSLEILEKYMDQAQSENL
                     IPYEPTLGQYLTAEEITERYANMKKWYEDHGHFWVGTGPYYLDQVFTTEKSLVLKHNA
                     EYVDASDRWSRFSAPKLASTILDGPGQVTAGQEAVFDVTVNLGDAAYANADIKLVKYI
                     LYDTTGAVVSVGEAQAVGEGQFQVTLDAEATSKLQTGSAKLEVAVVPIPVAIPSFTSF
                     DFVVIK"
     gene            22180..25938
                     /locus_tag="PL000_06285"
     CDS             22180..25938
                     /locus_tag="PL000_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNC88539.1"
                     /translation="MRKYFVYAIFLLTLLGNSCVAAPDTLKPPLPVVFDEPQSGLNSS
                     RNSRSTPSYSQNIRFEHFSLEEGLSQSTITVIFQDSKGFLWIGTEDGLNRYDGYTFKI
                     YKPNPEDPNTLTDRWVTALAEDPEGNLWIGTRLGGLNRYDPETGQFSHLLYSNTNSAG
                     ISHNHITALSADKDGIWIGTKNGLDYLSYGSQRIEHFRASSVNPNSLSGNWISAIIQD
                     ANGNLWVGTQDSGLNFYDKTKKSFSVFKHSDDVFTSISNNHITSILIDNRGVLWVGTA
                     NGLNNSESAGRYFTHYVNVDGDSDSLSGDVVYAIYQDRSLGIWIGTDNGLNRYDPQRE
                     SFVHHNHQPTTPNSLANDTVISIFEDNSNVLWVGTDGGGLNKYNRQQDHFAYYRANPD
                     DPTSLSGNFIMPIVVGENGTVWVGAYGTGLNRLNAISNQFRRYVHDPNNSNSISTNDV
                     TALKIDSSGNLWIGTSIGLDRLNADTGQFTHFVPDINNPESLSAAPVNVIYEDHSNTI
                     WVGTSRGLDQFDPQTQVFIKYDTEDNDPNSFDNNQITSMLEDENLNFWIGTFDDGIKR
                     FNPSNGEITSYTNNPDNPSSLGSNAVLCLHIDRAGTLWIGTAGGGLNRYNPETDSFVR
                     FTEKDGLPNDVIYGILEDKNGFLWASTNFGITRFDPKTNTFRNFTASDGLQSNEFHQN
                     AFAIDLKGNLYFGGNNGFNVFTPDAIDSDTVAPKIVLTSLTRDRTPISNNIPSESIKE
                     ITLQWPQNSFEFEFAALAYEQPSKNQYAYILENFEANWNMIGTQRNGRYTNLPGGSYT
                     LHLLGANSDGVWNKEGVSIRVTVIPPIWETWWFRSSLLVFLAASVFLGYRWRVKRIEA
                     RNRELERLVQRRTGDLEKRTREIEALYQADERILRNVTLIQVFQTLVDVSVSMLKADR
                     SMVFIWNEEKNKITPRVSYGFRTETLYALHFDQGEGLVGQTMKTGETLIVMDIKRGTL
                     RGDVETVIRREGIQSFAHFPIVVDGNVIAIFNVAYTRPNAFTDDTIRLFTSLVNRAAL
                     SIANMQLFEQTKDLAVMEERNRLARDLHDSAKQKAFAALAQLGTANGILKTKPNDVKV
                     HLTEAETLVFEVIQELTFLIQEIYPIALQEKGLPTTLREYIFEWENRNDTEVKLTIRN
                     ERPLPLEIEQAVYRIIQEALANVARHSKAKRADVSLVYNSDSLQIMVADDGHGFDMNQ
                     RAKGMGFRSMRERISSVRGTIQIQSAPGQGTRLIAQLPIKSPGKEAST"
     gene            25935..26606
                     /locus_tag="PL000_06290"
     CDS             25935..26606
                     /locus_tag="PL000_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88540.1"
                     /translation="MKHHRTSILIADDHEVVRNGIRSYLETLSDFQVVGEASSGEEAL
                     LMVAELIPDIVLLDLIMPGMDGIETTRRIKQTSPRTQVVVLTSYHEDVHIFPALKAGA
                     IAYILKDMKMEKLSEVLHRAVQGEVTLHPRVAARVLQNIRGENGEEQPLFTDLTDRET
                     DVLKLIANGLTNSQIAEKLVISENTVKGHVSNILSKLHLADRTKLAVYAWQQGIVNRD
                     SAPRE"
     gene            complement(26669..27439)
                     /locus_tag="PL000_06295"
     CDS             complement(26669..27439)
                     /locus_tag="PL000_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNC88541.1"
                     /translation="MNNPKELSKRQARREQIRRQSQRQRFIGIGLVVLGALAVAFLII
                     WPNLKPITGIISVEPNPRPQADANNMGDPNAPVKLVEYSDYQCPFCERFASETEAKLV
                     EAYVSTGKLYFTYRSAGNWVSDNIRGGKTESEDAAKAAYCAGDQNLYWEMHDMLFANV
                     LGEDVDSFTDRRLAAIAEQTGVDMTQFNECYSSGKFDDQVFQDGKDALTAGVRGTPGF
                     VLSYVNASGEEVTQLIEGAQPFDVFAQAIDAALAAAGQ"
     gene            complement(27529..28368)
                     /locus_tag="PL000_06300"
     CDS             complement(27529..28368)
                     /locus_tag="PL000_06300"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNC88542.1"
                     /translation="MKHFETSWKAHDGLEVFAQGWEPTVLQPKGVVCLVHGLGEHTSR
                     YAHVAEAFSREGLILFGPDLRGHGRSGGKRGHINSIEDFMTVIDQLLEQARNRYPNLP
                     IILYGHSLGGVLVLHYTLLRKPNIKGVIATSSGLRTALEDQKVKVMLAKVLGSVAPSV
                     TIPSGLDPKTISRDEKVIQAYINDPLVHDQMSFGFGKIMIGVTSWTLAHASEFPLPLL
                     LMHGKEDHLTFSAGSSEFAAPLKERCTLVLWDGAYHELHNEPEKAEVFKTMALWMDAR
                     LRE"
     gene            complement(28365..28976)
                     /locus_tag="PL000_06305"
     CDS             complement(28365..28976)
                     /locus_tag="PL000_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88543.1"
                     /translation="MTEIGFPVHILKRFVETLSAEMGHETLTGALTNAGLPAEWAHSA
                     HFSALDDQRAANVYSRLQSALRKYYGRGARSTLLWIGVKLWEQLLNDSAFGIKAQAAI
                     IQRLPKGLRRKPALELLARILSATSGNVSVHSLDLDLLFVDHASPTAMEQTDTAPICF
                     VTLGLIRECLFWAVGQEHDIEERACRAMGARQCEFKISIGGEA"
     gene            complement(28984..29841)
                     /locus_tag="PL000_06310"
     CDS             complement(28984..29841)
                     /locus_tag="PL000_06310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88544.1"
                     /translation="MININLQARRMVAEDYNQVSSLVYYEANNHRHLDWHSPLERINL
                     PNCWVLAEGASIHAVLICPEDPPNVAWVSFFGYSPSLSGAEAWSLLWEKARSDLASEN
                     RPLKVAAIVVRQWFQPLLLSSGFINQQNIVLLELRNEDIRYFLTPHGVRIRPMTDADI
                     PMVAKLDLAAFGDFWHNTASTLQRARLQCASATVAEDDSGVIGYQLSTRNPLGAHLAR
                     LGVSPEAQGRGVGAALVSDLIQSFGGAQLHRLSVNTQSDNEASLALYKKMGFVRTGEY
                     FPVLVYPLG"
     gene            complement(29838..30359)
                     /locus_tag="PL000_06315"
     CDS             complement(29838..30359)
                     /locus_tag="PL000_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88545.1"
                     /translation="MTIEPAQLAEQGKQAFRNKQFDESAELFRQAAEGFSLGRNGLLA
                     AEMKNNMSVALLQAGKAQESLDAALGTDLVFAGAKDIKSQGMALGNQAAALEALNRFD
                     EAIAAYERSAELFAEVGEGDLRAMVMKSAAAIKLKTGKVTESAFKMMGSLDAKDNPGF
                     FERILKFFLRFIK"
     gene            complement(30414..31088)
                     /locus_tag="PL000_06320"
     CDS             complement(30414..31088)
                     /locus_tag="PL000_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC88546.1"
                     /translation="MNNAQIIIREKKLGLLIRDARMAERRSIKECADAIGVKPGLFRA
                     YEEGRRSPSLPELETLVYYLKLPITHFWGRETMSETSSPAETLDTAQLIALRQRMIGA
                     LLRQERNKINMSLRQLAVDTGIKSARLNLYELGERPISVPELESILAVLGSRIETFFD
                     QNGPVGQWMTSQRAMQKFLELPEEIQNFVCQPVNRPYLELAMKLSDMSKEKLRSVAEG
                     LLDITL"
     gene            complement(31218..32531)
                     /locus_tag="PL000_06325"
     CDS             complement(31218..32531)
                     /locus_tag="PL000_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNC88547.1"
                     /translation="MTTVTPSSRLTGQDLSRLKKYLADNLMSAMGSERVPAAERSAFV
                     KKNIVHVYEQTQLKLPEDLRKQIFEQVLNDVLGYGPIQSLLDDEDVSEIMVNGPKKVF
                     VEKKGQLTKSTVTFDDDDHVMRIIDRIILPLGRHVDADSPTVDARMPDGSRVNAVVRP
                     VAIDGPSITIRKFRKDKLKPEDLINFGSLTRQMADFLQACVKARFNIIISGGTGSGKT
                     TLLNVLSGYIPENERIITIEDAAELQLQQDHVMRMETKVANAEGLHAVTIRELVKNSL
                     RMRPDRIVVGEVRGGEALDMLQAMNTGHDGSLTTVHANTPRDAISRIETLVLMAGMDL
                     PIKVVRQQISSAVDLIVQQTRLKDGQRKVTAVTEVAGMEGEIIVLTDVFKFIQTGVTT
                     EGKVLGEISATGIRPNFTPRLEAAGFKLTADIFAPKASANQGSRR"
     gene            complement(32534..32824)
                     /locus_tag="PL000_06330"
     CDS             complement(32534..32824)
                     /locus_tag="PL000_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88548.1"
                     /translation="MTIEYDEKGKFYTEVVTKVPTAVVIQTTTHLIRGLVHVRQGERL
                     KDELEMDERFIAVTNFTIQDANEKVLFSGAFLAILRAQIIWITPADESSQGG"
     gene            complement(32952..34106)
                     /locus_tag="PL000_06335"
     CDS             complement(32952..34106)
                     /locus_tag="PL000_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88549.1"
                     /translation="MSEKKILIVDADVASRNFISRNLQAQKYEIIQTGSGKEGLIHAW
                     RDRPDLMVIDPTVPDITGEEVARKLKQDARTTNMPLIALSSDLGVARIKSCLDAGFSE
                     YIAKSGQAMATLTDAINRLLGISSALVKEGGLLMAFISAKGGVGTSSLCANLGLTVCQ
                     NQPEARVAVVDLVLPIGSIGSIVGYEGPLNIVTASDMPEEKITPDYFRDQLKEAGIWR
                     FHLLSGSPDPESSNHLKVGRIWNVIAALKASYDYVLIDIGRALSKISLPILQHADLVA
                     LIISTDVSALPLTKTVLDYMKTKGVEPATIFPILNRASGLEGLSKPDAEKALDIQIKA
                     AMPYLGSSFAFASSHHQPFSLKFPNDTASFVFLETAKEMAVLAQKLRTGQ"
     gene            complement(34150..34845)
                     /locus_tag="PL000_06340"
     CDS             complement(34150..34845)
                     /locus_tag="PL000_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88550.1"
                     /translation="MSKRILIVDDEPRYLRLLEANLRTEGYEVATAQDGVQALDVFSV
                     QPIDLVLLDVMMPRLDGFGVCQRLREFSNVPIVILTARGEEQDRVRGLDLGADDYLVK
                     PFSATELLARVRAVLRRAQAPTESGQARFFTHDDLKIDFARAEVWRGEEPVSLSATEY
                     RLLLQFTHNVGKILTSEDLLTSVWGVEYKNDKEILWVSIARLRQKLEADAHSPRHIVT
                     RSGLGYLMPPTEI"
     gene            complement(34879..36066)
                     /locus_tag="PL000_06345"
     CDS             complement(34879..36066)
                     /locus_tag="PL000_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC88551.1"
                     /translation="MDLATLTFLHKTFQEVQIQQDWRAALDALFVSMRGSFVFDNAAI
                     YLEDPRTKGLEVVYARAVGRGKAAEADSMWGEGIANEVLSKGRMILKEPRKNLNYANR
                     LTEAHLLGFPMYVGGKLNGALVFIRFGGPEYSNLHIQIASLQSFWATALIERRDLQEA
                     RTELDSVQRQMRLQDDFVSTISHELRTPLGFIKGYSTSLLRQDTTWDETTQREFLNII
                     DEEADRLARLIENMLESARLQSKTLQFKFSPIRIDALARDVAMRIHTHHPELKVNFET
                     DSLPSIRGDGMRIAQVFENLFSNALKYAPNSEITIAMRMQGDKIRVSFADKGEGIPED
                     YLPFLFERFYRVPGERTVTGTGLGLYICKQIVMAHHGNIWVESELDKGTTFIIELPVD
                     STL"
     gene            complement(36069..>37106)
                     /locus_tag="PL000_06350"
     CDS             complement(36069..>37106)
                     /locus_tag="PL000_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88552.1"
                     /translation="LPRNPMSQNLPKLTPEMLIPRMGEYIVQKGLITEADLQRALTYQ
                     QDQIAKGNHYLLGQSLMDLNLLDRDTLDQVVTEQILQLRSALQSANRNLEQRVKERTA
                     ELNEALQRLSELSQLKSNFVSNISHELRTPLTHVKGYIELLVAGSLGEISEEQRHALQ
                     VSQRATAKLEGMIEDLIMFSLASKGEMSMKLQRVDIRRIVSLAAKSALPKAEERGVQV
                     LVSALENIPSVQADPEKIGWVVSQLLDNGIKFTPSGGSVVISLKEEGHNLVVLTVTDT
                     GIGIPSNKIKEIFEPFHQLDGSSTRHFGGTGLGLSLVRQIVEAHGSLLDVQSAEGKGS
                     VFKFPLLALGD"
     assembly_gap    37107..37206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(37315..38904)
                     /locus_tag="PL000_06355"
     CDS             complement(37315..38904)
                     /locus_tag="PL000_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC88553.1"
                     /translation="MKLLYNANIHTLDSSHPQASAILIEGGRIVAVGEKDTLEAIAHG
                     RVEKQDMGGRTILPGLTDAHLHLQHYGLSLQKVDCETKTKAECLRRVAERAQKAKPGA
                     WILGHGWNQNEWTDDNAFPAASELDAIAPNNPVYLTAKSLHMGWANTAALQRASITND
                     TPDPKNGAIQRDNTGRATGILLETAMSLVEKTIPEPTISEIESAIENAQSILWKMGLT
                     GVHDFDRRDSFMALQHLHAQKKLKLRVNKNIPVESVDHANALGLRTGFGDDWLWIGSV
                     KAFMDGALGPHTAAMFQPYQDEPENRGILNMDGEELFEHCRKAADVGLSMTVHAIGDR
                     ANHEVLNAYQQLRAYETEKGLPHLRHRIEHVQVIHPDDAPRFAQLNVIASMQPIHAPS
                     DMFAADRFWGDRSAHAYAWRTQLNHGATLAFGSDAPVESPNPFLGLHAAVTRRRADGS
                     PSAEGWYPEQKLTLAEALSAYTVGAAYAANAENRLGKLAQGYLADLIVLEENIFSADP
                     QSLLTIKAKSTMINGEWVLED"
BASE COUNT         9908 a        10754 c         9448 g         8801 t
ORIGIN      
        1 tttatctttt atccgcacac gaaagcggat ttcaagcagg tattttaatg gggtttacag
       61 aaggaggagg tggacgaaag tcctaatctt cgatctcaca actcttcatt caggtgcaaa
      121 cacttcagca caaccgttac tttttgagtt cttttgtact cagcaaataa agaataccat
      181 aacctcccaa aaacactcca gccaagatcg caatgtattt caagtatcct gtatcggaat
      241 aacctatatc catcagccaa tctggcatag gacgcggcaa ttcgctggcg gtgctgtcga
      301 tccagtacag atcctcactt gaaacccata cttttccgtc ggcaccaagc acaaaagaag
      361 agcggccaga tctggggtag ttagaatttt ctttgttgta ctgcacaaca agttctgcaa
      421 caccgctcca aacaggccgc agtactgtaa ctccgcctcc atatgaatca tccaaaatcc
      481 ataacctgtc atgcgaatca accagcaagg aatcataatc agaaaattcc cctaaaagcg
      541 gaatttcagt gatcaaagcc ttttcatcgt cttgaatttg aacaaaataa ctttcaactt
      601 cttccgtctg cgtagtatag aaaataaaga gacgccccac atcgtccacg atgaagtctt
      661 ttacctgaag aaaatcgaag ggcacatcat aagacttctc cagccattgt ccatctttca
      721 acagaaatat tctggcttca ttctcattcc cagcatgaac caaaactgtc tcatccgcca
      781 aaatggaaag ggaatagggc tcatatcctt tttgaactgc agattctggg agttggatca
      841 catcaacacc aggcctgcca gctgttgcac caaccaaaac attgggtttg ttatctccgt
      901 acaaaaccag ccaggcttgt tccgtcgtac tcacaaatct cttacgatag atggcagcct
      961 tataaacccc gaaatacttc cattgcaccc atttgccatc ctgccaggtg taattgattt
     1021 caccagtcat cagagttggc tggccattag aatcggacag aataatcgtt cctcgagggt
     1081 tttgcagcgg aagcggccat ctctgctcct gatcatcaaa atgaacaatc agattgtcac
     1141 cattaaaatc agtagaagca atccaaggtg agttcttaga agtgattgaa aagtcgagca
     1201 aaaactcgtt ccagggaagt ctatgaacat tccatccgcc ctgaaaaaaa ccgtatacac
     1261 caagaactgg aatcgttatg agggggagaa acaataccat ccccgcatcg ttaatcttta
     1321 caaggatgga atttttttcc atcttttctg gcaatttttc atctatcact gttcctggca
     1381 ggtgggtttt taggagttcc atcactctat ctgccccacc ttcgatatct gccaaaacaa
     1441 atttaaaatc ctcttcattt ttagagcgta caacaatgac ccctggaaat aaatactgca
     1501 ctgataatac gtcactgtac agaatccgcc gcgggcggga tgagtaaaaa cccttatttt
     1561 ggttttcctc aatcgcctcg cttgtaaaca caatacgata tttaagttct ttttttatac
     1621 ggacaaaata aaaccatata aaaaaaacaa gcactccaac agtaaaaaga ttaacgaaac
     1681 tcaactccag cagggatttc aacgatgccc gtggcggatc aaacaaaaaa gttaacggaa
     1741 gaattccaat caatccagac caaagcggaa gaattagcgg acgacgattt aactttagcg
     1801 tattcatttt cctccttaca ctcaatgcaa catagttttc aaattaattt taccttctgt
     1861 cccgctaaac ctccaccctc accaccaacg ccgtcctcgc ccactcactc ggacttctgc
     1921 cttcccactc gttgtacttg atcgccatct tcatccgcac caacgtctcg gtctgtccat
     1981 ctttcagatc cagcagcttc gccaccgcag ggaaggtctg tcccgcgatt cgcactgtca
     2041 cattcgggtc tttgagcaaa ttcctcaccc aatcagacct gtccatattg cccgataaaa
     2101 gataaagcgc cccttcatgc acacagaacc aaatctcgat ctcatgcgga tcccctgttt
     2161 tgcgtccgcg ggtggtgagg tagcaatatt gctctttttc aaattgcgtc aggtcaacgg
     2221 ccatggtcaa gcctccttct cgttcgaaag gaaatacgtc tgtgcaggga gcaggatatg
     2281 cacccgtttt tcagtggcat acaccgtggt acagcgtccg cgcggcaggc gtttaatgag
     2341 cgaagaatcg tacaacaaaa tggaacggct cgtgatcttc tcgatcaccg tccaatgatc
     2401 gtgataaccc tccaacccca aaatgatgga acgtcccggc gtgccatcca gaaatgcctg
     2461 catcgaatgc caaaacgtgg tcaggtcggg gttcggcacc ccgcgccacg ggatctgcac
     2521 attcgaaatc cgcttcttgc ccaccacctt gtttaagatc aacagcatct ggtgatgcaa
     2581 gatccccccg atcagaaact tacccagcaa accgcgccgc gaaagataat ggatcaagtc
     2641 atcgaacaac tgctgcgtct cttcatcgga agaatgattg ataaaccgct ccgcattgac
     2701 aatgctgtac aacccgcaca acgaatccaa cccaccctgt tgaaaaggcg gaaaccccga
     2761 actaatgcgt gaagaattcg tcataaacaa taaccctaaa tcagatcact caaccaaccg
     2821 ccacaacaaa atacccgccg ccaaagccaa tgccgcccca aagaaaaaag gagccgaagc
     2881 gccgaatcca gcccatgaac ccagaccctg ccacagcagt cccgcaagaa gcgaggcagg
     2941 cagagccgtg atcccgatcg ccgcattaaa ccatccatac gccgccccgc gctgcgcctc
     3001 tggcaccaga tccgcaacaa aggcctttgc cacaccctct gtcgccgcgt aataaattcc
     3061 atacaaacca aacagggccc agatctgcca gcccgcaccc gcaaacgcaa accccaaata
     3121 caccaaccca tacgccatcc acccgccgat gatcaaccgc ctgcgcccca ctttatctga
     3181 aagcgccccc aacggccccg aaagaatcgt ataaatcgcc gtgaaggtca tcaacatgaa
     3241 caacgtctgc agcacattca acccgcgttc ctgtgcgcgc aaaataatga aagaatcgga
     3301 tgaattcccc agcgtgaaaa gaatcgtgat caacaggaat tttccaaacc gtggatcgat
     3361 ctccttaaaa gacagacgct tcttctccgc gcctttgcct gatgcagcaa catcggcagc
     3421 ccccaccccg agaacgatca ccgccaacac cgcagggatg atgctcgcca acaccgccgt
     3481 ttgaaacgct tcgcgtgtga tctgcaatgc accctgctgg gtcagccaca cgatcaaagc
     3541 ggcaatggcg atccccaaaa aagcccccgc cgtatcgcca gctcggtgca aaccgaaagc
     3601 caatccgcgg ttggtttcat cggtactggc ggccaccaac gcatcacgcg gtgccgtacg
     3661 gatccccttc cctacacggt ctgaaaaccg caccccgagc acccatcccc acgaattcgc
     3721 aaaatacaaa aagggcttgg caaaagtgga gatcgcatat cccagcaccg ccaatccctt
     3781 gcgctgtccc agcttatccg aaagcgcccc cgaatacacc ttcaacaaac tcgccgttgt
     3841 ttccgcaatt ccatcgatca gcccaatgaa cgccatccgc acccccaaca cgttcgaaag
     3901 aaaaagcgga atcaaataca caagcatctc cgaagaaatg tctgtgagaa aagaggtcac
     3961 ggtcacgatc caaatatttg gatgtaattt tcgaagtgta ctccgcggtt tcattttttc
     4021 ctttttattg ttcgttcatc atccatcact cccccatcaa aacctcaagc atccatcatt
     4081 gccatatcaa tccttcttgc caaaataccc aaacccgatc aacaccgcag acatcagcgc
     4141 cgtggcgacc ccggtccgca acaggtcatt cgtatccttc acttccaaca gcttctgcag
     4201 gaacttcacc cccatcacca aaacgatcat actgctgagt tttgccttca attcatacag
     4261 gttgtgggcc agcatccact ccggcaattc caacggagaa atgaacaatt catacaaact
     4321 tacggcaaag atcaaaatgg cagtggcgat caaaaaactg tccacgatct caatgaactc
     4381 gatggtgatc gccgcatcct tgcccattga ctcaaacaca agcagcaccg ccgcggcagt
     4441 tttcagggtc ccccacgcaa aagccgccac cgccgcgatc aacagtgaaa gcacacccac
     4501 aattgcaaga tatttggatt tctcaagtaa ggttttcatg gtgtccgttt tattcgccgt
     4561 acatctgacg atacaaagcg cgcaggtttc gtcggtgcaa agtctctgcc aggccgccaa
     4621 ggtacacgcg gctcttcccg cattcttcga tggtgatcgc gtccagatat tcatctgcgt
     4681 tcttgcgctt gcccgcagcc ttcaccagtt ttttgatatt gttcaggtag ttcatattct
     4741 ctttgaccgc cgcatcgatt tcgccgcgca aaatgatatc gccatgaccc tgaatgatat
     4801 tctccagtcc catacgcccg atccgcttga ccgaggagat catctcgtca atatctccat
     4861 ccacgatgta aggcagggac atgaaagcat ccccggcaaa aagcacgcga tcctcttcca
     4921 ccaaaaccga aatgccgtct tcgctatgcc caaaagactg cgaaaacacc agattcttct
     4981 tgcccacccg catggtcaat tcccccgaat cgaaggttaa atgcggcgga atgatcttca
     5041 cctgccgcaa attcgggttt tgtttacgcg caccctccaa agaaggaatt ccatgctcga
     5101 tcagcagttc acgacatttc gcatgcccga tcacagtggc accggggaag aaacaattgc
     5161 cccaggtatg gtcggcatgg taatgtgtat tgatcacata acgcacttga acgccaagtt
     5221 catgttcaat aaactcgcgc ataccaagtg tctcttcagg cagggccagc gtgtcaatca
     5281 caacggccca ctgcgggcca acaataaccc cggcagttac ctgagcataa acttcgcttt
     5341 gaaaccagaa cacattttcc gaaactcgtt cacgatgcat aaatcaccct tgtatggata
     5401 tattttcatt attaatagca tactcccgcc caaattacaa gttgattaga caaattgaaa
     5461 gattttcatt aatgtaaacc gccctaaaaa atttgacttg attagccctc ttataagtga
     5521 taaactctac tgcaaatgcc tgcggatatt cttgtagtca cctcttcttc acaacttgga
     5581 gagtccatcc gtcgcatcct ggaagagacg gatctctacc gtatccatgt tgtcaccaat
     5641 aaatcctctg ccatcgttcg cgcagatgaa gttggtgcgc ctcttgccat gcttgatctt
     5701 gcccttggag aaggtgtgat tcgagagatc ggacaatctc tccgcaccat tcgtcccggc
     5761 atcaacctca tcatcctttg cgatgaaaac atcacgcccc ctccgtttga cgaattgcgc
     5821 ccttggattc ttgttcgtaa acctttcaag atgtcaaatt ttatgaccgc gatcagtcaa
     5881 ccccaaacca caacagcaac accccccacc cccgctacta caacgtcaac cccggcagcc
     5941 gcgccgcaaa atacctccac gcccactctg ccctggctca gtgacccgaa ccgggcggcg
     6001 cagcacctta cccgcctcac gcttgagtct tctgcacagg ccgcattgat cactcgtaaa
     6061 agtgacctgt gggcctatgc gggcagtctt tcccagggtg cagcaaagga agtggcacaa
     6121 accatcaccc gcaattggga tgggcaaaaa ggatttgacc tgttgcgctt catccgcctc
     6181 gaaagcacca aagcggaaca catgctttac gccacccgcc tcgctgccga caccgtgctt
     6241 gctcttgttt tcgacgcaga aactcccttt agcaccatcc gcgcacaggc cagtcaactt
     6301 gtcaccaatc tcgataacga aaccgcggaa ccgcagaaac catctcagcc gttcaatgcg
     6361 gaaaaacaag ttgttgaagc agatgccgat gacggtgatg cgccaaacat cccgcttatt
     6421 tcagacatcc tcaacaacgt accgacaccc aaccccgaac ccgccacaac ccgcgatttc
     6481 aacctgcccc gtaaaaaaga aacacaggaa gccttcgacc cgaatcagac ccgaatttca
     6541 gaatcgcttt cgaatgcatc cgttttcaaa cgagacccgt ctcctgcagt ccattacggc
     6601 agcataccgg tcctgaataa aaagccagcc ccctccattc cagaagttca ggttcagcct
     6661 caaaaccagg ctgtgttgaa cgaagttgac gttacagccc catcacgcat tcaacaaagg
     6721 cctgaaaccc cggtggccaa acccgctccc ggcgaacagg aagtcacccg cgaaagcccc
     6781 accaccgagg ccgcacggaa actcgtcgta gaaccgacaa cggcaggcct gtaccatttg
     6841 acctacgcct gtctgcttgt accgcgtttt tcagctcact accttaccgg tgatctggct
     6901 gaccagttgg gagaatggct gcccaatatt tgcatcgcct tcggctggcg actggaattc
     6961 ctctcggtac gccccgaata tttgcaatgg gtcgtcaacg tacagccgaa tacctctcca
     7021 ggttatctca tgcgcatcat gcgccagcaa acctctgaaa agatattcac cgacttcacc
     7081 cgtttgaaga gggaaaatcc ctccggtgat ttctgggcgc ctggctatct catcatgggc
     7141 ggaacccagc cccacccgca gcaattggtg cgtgattaca tccgccagac ccgccaaaga
     7201 cagggacagg aactcccatc ggaaccgaaa cgcaagtaag ttttccgaca caaacgaagc
     7261 gcagagtata atcactctgc gcttttattt ttcagctgtt tcaaaaaatg gacctcttgc
     7321 aaaagcctaa aatcttaaag agaaaaacga tgaaggctct gcctaaaagt tttttcagtt
     7381 acccgcttat gtaagaagtc aaacaaaaag agaatcccac atgcccccta ccctttatct
     7441 cattgacgga cacgccctcg cctatcgcat gtactttgca cttacagcag gcggaggcag
     7501 tcaacgctgg caaaactcca aaggcgagcc cacggctggc atttacggct ttgcgcgtga
     7561 actggtgcgc gtgctcgaac aggaaaaacc agaatacctc gctgttgcat ttgatgtagg
     7621 caagaccttc cgcgacgata ttttccccga atacaagggc acccgtgaaa aaatgcccga
     7681 tgaccttcgt ccgcagatcg agcgcatccg ccagatggtg gatatgttca atattccgcg
     7741 cctcgaaatg gacggctacg aagccgatga tgttctcggc agcgccgccc gcattgccgc
     7801 taaaaaaggg ctcggggtga agatcatcac aggtgacaga gacctgcttc agttggtcaa
     7861 cgagcgcacg gctgtctatc ttgctggtga cgaccagacc tacatcaccg atgcagatgt
     7921 gatcaaaaaa ttaggcgtgc gcccgactca ggtggtggat tacaaagcca tcgtcggcga
     7981 tacttccgat aatattcccg gggtgaaggg tgtgggtgaa aagaccgcca ttgccctgct
     8041 cgaaaaattc ggcacgcttg atgccatcta cgccaacctc gatcaggttg aaaatcgctg
     8101 gaagggcaag ttcgaagcaa gcaaagatgc ggcctacatg agctacaaac ttgcaaagat
     8161 cgaggtggac ctgaagatcg atctcaacct tgaccatgcc gcggtcaggc cgtttgattc
     8221 gtctgcattg gcggctttct ttcaggaaat ggaattcaag accctgctca acaaactgcc
     8281 cgccatcagt ggaagcgcac cctcagtgga gatcgctgct gcgccgaaaa aaggcaaggc
     8341 aggcgagcag atgtccatgt ttgtaaatgc acctgtcatc gtttcaacac catcgaagat
     8401 cgaagtgacg gttgtggaca cagacgaaaa actgggcgca ctaaaaaacg aactcacccg
     8461 ggcgaaggtc atctcttttg acacagaaac aacttcaacc gaggaagtga ccgctgaact
     8521 cgtcggcatt tcgctggcgg tcaaagaggg tcatgggtat tacattcccg ttggtcatgt
     8581 ttctggaaac aaccttgaaa tcaaaaaagt gatcgctgcg ctcacgcccg ccatgaccga
     8641 ttcgaaaatc ggcaaagtgg cgcacaatgc aaaatacgat tacatcatgc tggcgcggta
     8701 tgggttggtc atctcgccgc tcaccttcga tacgatgctg gctgaattca ttgtggatcc
     8761 atcatcgcgc aacctcggct tgaagaattt atcagaatac cgccttggtg aagagatgac
     8821 tcacatcgaa gaactgatcg gcaaaggcaa aaaacagatc agcatggcag aagtggcaat
     8881 tgaaagcgtc gccaactatg ccgccgccga tgcagagacc accctgcgcc tgatgcccat
     8941 catgcaaaag gaattacagc gtgtgaacgg cacaaaactg ctcgatgaaa ttgacatgcc
     9001 gctcacccct gtcctcgcgg aaatggaaat gacgggcgcg ctgatcgaca cgaaattttt
     9061 cgcggcgatg tcgcaggaac tgggtcagcg tttggcagag atcgaaaagg agatcttcca
     9121 gctggcgggc aagaccttca acttgaattc gccgcagcaa ttatcggatg tgctcttcaa
     9181 ccatttgcgc ctcgaaccac ccgacaaagg ccgcaaaaca acaacaggct tttactccac
     9241 ttcggcagat gtgctcgaag ccttgcgcgg aaagcacccc gcactcgatt tcattcttga
     9301 gcaccgtgaa ctctccaaac tcaaatccac ctatgtggat gccctgcccg ccgcaatgga
     9361 cccgaatacc ggccgcgtgc atacctcata cagccagatc ggcgcggtga cggggcgcct
     9421 ttcttcgaac aaccccaacc tgcaaaacat ccccatccgc accgaaacag ggaggcgcgt
     9481 ccgcaacggc ttcattgcag acaagggcaa tgtgctgctt tcagtggatt attcgcaaat
     9541 tgaattgcgc atcgtggcgc acatggcaca ggatgaagcc atgctcaaag ccttccgcgc
     9601 tggtgaggat attcacgcca ccaccgccgc cgccatctac gacatcgagt tggacaatgt
     9661 gaccaaagat atgcgccgcc atgccaaggc catcaacttc ggtctcatct acggcatgtc
     9721 ggcatttggc ctcacccgct ccaccgacct gaccctggcc gaagccgagg aattcgtcaa
     9781 agcctatttc acgaaattcc ccggtgtgaa aaaatatctc gatggcatgc gccgtcaggc
     9841 cgccgagatc ggatacgtgg aaaccctgct cggacgcaaa cgctacttcc ccgctttgca
     9901 gggcaagatc aacatgcagt tgaagaaccg tgaagaacgc gaagccatca atgcccccat
     9961 tcaaggcact gcggcagaca tcatgaaact cgccatgctg aagatcccct ccgcgttgca
    10021 ggcggcgggt ttgaaaggca aaatgctttt gcaggtacac gatgaactcg tgctcgaatg
    10081 ccccaaaaag gaagtggaaa agaccgcgaa ggtcgtgcag gaaaccatgg ccgaggccta
    10141 cccgatgagc attcccctcg aaacagaagc aagagcgggc gcgagttggg gcgaaatgac
    10201 cgtactaaag tagcccgccc cgttgcttca tccagtctgg agagacggtt ataattgcgt
    10261 gataattttt cattcagcgc acatgcgaaa gtagaataaa cactatgccc ggacgagaag
    10321 atgtcttcca aaaagcaatg aacgacggcc attcagcggc ctgggatcaa gagtgggata
    10381 aagcctgcgc tgcctatcgc cgcgctttgg aggaatttcc cgaccatcca aaggcgttga
    10441 acagtctggg gcttgcgctt taccagctcg gcaattttga agaagccttg cagatctaca
    10501 ggaatgtggt gcggctctcc cccgatgacc ccatccccat ggaaaaggtt tcgcaaatct
    10561 cagaacgcat cggcgacctg aaaaccgccg tggaagccgc catgcgcgcc ggtgacctct
    10621 tcctgcaaca gcgcgatact gaaaaggcac tcgaaaactg ggtgcgtgtc acaaacgtca
    10681 acccggaaaa tgccatcgcc cattcacgcc ttgcacaagt ccatgaaaga ctcgggcaca
    10741 tgcaacaggc cgtcaccgaa tacctcgcca tcgccagcgt tatccaacgt gcaggcaatg
    10801 ccgagaaaac acaagagatg gtcagcaagg ccctctccct tttgccagac agccccgaag
    10861 ccaaacaggc acagacctca ttgaagatgg gacaattgct ccccaaaccc atgcgcggaa
    10921 aaggcggaac gggccccatt cgcatggcga tggtcaagca gctgcaaaaa ccaaccgtat
    10981 cgcaaagcgc ctccgggctg gacccgatcg ctgaggcgcg ccaaaaagca ttgactcaac
    11041 tggcagagct tctcttcgaa ttctccgacg acaacccctc cgcacaggaa aaacgcggac
    11101 tgtccgccat catgaaaggc acaggcggcc tctccctgca acaatccgaa caaacaaaag
    11161 cggttctaca cctcggcctt gcaattgatg cccaaaccaa aggcaatgaa acccaggccg
    11221 ccgaagaaat ggaaggcgcg cttgaagcag gcttcaaaca tgcctcgctt tacttcacac
    11281 ttggtctgtt gcgtttcaaa ggcaaccgcc tcgaaagtgc ccaacgcttt ttgcaaaact
    11341 ccgtcaaaca caacgattac gccctgggca ctcgtctgct gttgggacaa ctgctcgtta
    11401 aaaaatcgct ctacgatgaa gccgccattg aataccttga agcgttgaaa ctcgccgact
    11461 cgatcaccgt ccccgccgaa aaagccgacg aggtcagcca gcaatacgag ccgctgatcg
    11521 aagcgcatca aagccagaaa gatgagaatg ttctgcgcaa agtctgcgaa aacatcaatg
    11581 gcctgctgat gcggcccgac tggcgcgaac aacttcacaa aacccgcgaa caaatgccga
    11641 ggcaggaagg cgaaatgctt gccccgctgg ccgatgtgat tctcgaagcg caatccagct
    11701 ccgtgcttga atcgatgaac cgcatcaatc aactggcccg catcggcagc ctgcgctccg
    11761 caatggacga agcctatcat gccgtgcaat atgcgcccac ctatcttccc ctccacgcgt
    11821 tgatgggtga tctgctcgtt caggaaaacc gcgatgcaga tgcaattgca aaatacagca
    11881 tcgttgcgca tctttatggg gtacgcggcg aagtaaatca agctacaaaa ctcctgcgcc
    11941 gcatcatcca acttgcgccc atggacctcg gttcacgcaa ccgcctgatc gaccaactcg
    12001 tcgcaagagg tcatgccgat gaagccgtgc gcgaatacct tgaactggca gccattcact
    12061 accgcctcgc cgaactcgac ctggcccgca aaacctacac caccgcactc agactcgtac
    12121 aacagggaaa tgccaaccgt gattggaatg cccacatcat gcagcgcatg gccgatattg
    12181 acctgcagcg gctggactgg aaacaggcac tccgcatcta cgaacaactc cgcagcatca
    12241 cccccgatga cgaagccacc cgccgccaat tggtagacct caacctgcgc atggcccaac
    12301 ccgacaaagc catgagcgaa ctcgagaact acatcaccta tctcgaaagc cagggcaaga
    12361 acgacctctc catcaccttc attcaagacc tcctgcagga acatcccgac caactcctgc
    12421 tcaaacgcgt actggcggca cgtctgcact ttgcaggccg caccgaagaa gccatctcca
    12481 tcctcgatgt cctcggcgaa aacctcatgc agacgggcga caaacacggt gcaatggaag
    12541 tcatcaacca gatcattttg atgaacccac ccaacgtatc agactaccgt caactgctca
    12601 tccaaatgca aggcaattag atttcatccc cgtcagataa aaatggcggg gatttttttc
    12661 gcctctcact cggaccgttg aacctgatcc tgcttcccat tttccaaaga acccccagca
    12721 gaaacattct cacagcgaag tgctataatc cgtgcgattt caacaacgga gaacaataaa
    12781 atgctaaaaa cccaaaacac cgccgtgatg aagtggcttt acgtttttgc cttcgtcgtg
    12841 gcttttctcg tcgtcttcgg gggatttgta cgccttaccc gctcgggcct ttccattgtg
    12901 gagtggaatc ccatcagcgg ggccatgccc cccagcggac agcaagcctg ggaagaggag
    12961 tttgcaaaat atcaggaaac ccctgagtac aaactcatca acaccggcat gaccctgcct
    13021 gaatacaaat tcatcttcta catggaatgg ttccatcgca atgttgcgcg tctggcgggt
    13081 ttgttctacg ccttccctgt gttctacttc ctcttcaaaa agaccattcc cttcaaagaa
    13141 tttggcatct actttgtaat gggcattctt ttcatttcac aggcggttgc aggctggatc
    13201 atggttgcca gcggactgga caaacgccct gccgtcagtc acttcaacct caccattcac
    13261 ctgcttctgg cgctcacatt gttcagcctc gccctgtgga ctgccttcgg gcacaagtac
    13321 ggttttcacg ataccagcaa gaaggcggcc tggtcacttc cctccaaact tgctgtattc
    13381 tcttttgtca ttctcgccat tcaggtttca tacggcgggt ttacagcagg gctcaaggct
    13441 gggcatgttt cagatacctg gccattaatg ctcggcaaat taattccccc caacctgttc
    13501 aactcgtgga tcaatatctt tgaatccccg caaaccatcg tcttcatcca ccgctggttc
    13561 gcctggctgg ggttgatcct cgtccctgct gtgtactatc tcgttaagaa gcaaaactat
    13621 cccgccgata tccaaaacgg acttaaatgg ttgatcggtg tggtggccct gcagatcacg
    13681 cttggcgtgc tcaccatcct gtcctacgta aatattgtga tcgcgctgat ccatcaagcc
    13741 aacgccatcg tactggttgg gctggcagtg ttcttcatcc acaggttcag ggcagtggac
    13801 aggaagtaaa agaaaaaaaa gtgaaaataa aaaagcgatg gcagattcat ctgccatcgc
    13861 ttttttattt aacagcccga attgttactc acggcggcgc gcggccatga aagttgcgcc
    13921 gatcatgcca agggcaaggc caaccaccga agaagcccac gaaaagaaat tgatcaagaa
    13981 cgtagactcg atcacgtgga tgaccatcat aaagggcagg gcaatgccca ggatcatcag
    14041 gaacaggcca agggtcaaaa ggaagcgtgg agatctcatt gcggttgata cagccaacag
    14101 gccacaaaat gttcgggttc aggttcaaaa tccacgggtt ctttttgatc gcacaaaccc
    14161 gcaatggccc gcgcacagcg tggatgaaaa cgacagccct tcggcgggtt caccaaactc
    14221 ggcggttcac cgggcggcac ttccttgtat gtatcggcgt tgcgggcatc ggggtcggag
    14281 gtggcggcga gcaatgccat ggtgtaggga tgcagcgggt tatcaagcag gcggcgggta
    14341 tcggcttttt cgatcaggtt gcctgcatac atcacaaaaa tacgcgagga aaaatatttc
    14401 actgtggaaa ggtcatgcgt gatgtaaatg accgagagtt gatgctgctc ctgaagggtt
    14461 cgcatcaact taaggatctc aacccgcacc gacgcatcca gcatggatac gggttcatcg
    14521 gccacgatca atttcggccc aaggatcatt gcccgcgcaa tgacaatgcg ttgctgctgg
    14581 ccgccgctca acatgtgcgg gaattttggc agaaagtcat tggcaggata cattttcact
    14641 tcatccattg ccttgcggat gcggtcaagc cgttccgttt tatccttcac accgctgatg
    14701 atgagcggct cttcaaggat ctgttgaatg gtcatgaaag gcggcagcgc gccatacgga
    14761 tcctgctgaa cataaccgac ctcgaaacga tattgccgca gaagattccc ctttaaacca
    14821 gccagattga ctccgccaaa aatgacttcg ccgctggtgg ggatattcaa cccgaggatg
    14881 gtcttcatca aactggattt tccgcatccg ctctcgccga caactgccac ggtttcccct
    14941 tcagccagat caaagctgac gccatccacc gccttgacgt agcccgcatg gccgaacccg
    15001 aagcgccgca gttcgtacca cacatgcaga tcacgaatgg atagcaaggt ttccttcggt
    15061 tcttcaaaat taactgtcat cttgttcacc tttcgtaatt cagtgatcat gtctgcattc
    15121 atggggatcc gtcactttct attttcagtt gccgagccga agcccgtttg caagcctttc
    15181 aaacattcca tcatgcccag atggcggcaa tcgtgcgtta gaaaaaccag caaattcaag
    15241 cggatattct gtaatttctt tttcaattca tcatccacat cgtcgaaatt tgggtaacgc
    15301 tcgtcgagcc gctccacaaa ttcttccact gcatcgtagg aacgctggag gtaatccacc
    15361 aattcctcct tgcgtggaat gggcagggtt tccaccgctt catcactcaa tccggtgcca
    15421 accgtggcat tcaaccccgt tgggaagccc cacttctgcg ccaaaccttc cttcacccaa
    15481 atctccagcg gggtgccaaa ctcgggcacc aattgaggtg tgcgttccaa aatgatcgct
    15541 ttgaggtaat ccgattcacg cgccagatgc cagagatgaa aaccgatgga ggtgctgtat
    15601 ccgcgcgggc gccaatgcat ctggtcatca tccagtcggg aggcaaaatc aagaaccact
    15661 ttgtgaatat ctttgaacat gtagagaacg tcataaagag cctgtggaga catattcttc
    15721 ctttttgtgt tctacgcgtg cagccagcac ttgaccttcc gattcgcttt ttggaaaaca
    15781 ggcggctcct cgctgcattt ttcaaaacga tacgggcagc gcgccgcaaa acggcagcct
    15841 ttcggcgggt tcatcaaact tggcggctgc cctgtaatga acatcgggtc tgaatcggtg
    15901 cgcaaacgcg gaacacttgc cattaacatc tgcgaatacg gatgcagagg ggcgggaaaa
    15961 aaatcttttg catcgcctgt ctccacgatc tggcccgcat acataatggc aacttcatca
    16021 gcgagttcgc tggaagtggc tatatcatgc gtaatgagaa taaaggaggt gccaagctca
    16081 tgcttgatgc gtttcaacac gttcatgatg ttcgcctgtg tgagcaggtc cagcgctgaa
    16141 gtcggttcat cgaggacaat gagactgggc gaagtgacga gggccatggc caacgccaca
    16201 cgctgacgca tcccaccgct caattcaaag ggatagcgtt ccacaaaatc ggcaggcaca
    16261 ccgacatgtt gaaacatttt gtaaaccagt ttgtaggcgt caactttgct caggcccaaa
    16321 tgcaccatgg caggttcagc cacctgatcg cccaccctca agacggggtt caatgaattc
    16381 atggcggctt gaggcaccaa tgacatgccc acccagcgca cattctgtcg gtactcctca
    16441 ttgtccaggg ccattacatc ggtaccctgc aaatagacat gcccggagta tttatccaca
    16501 ttgcggggga gcaggcgcaa caaggccttt gagagcgagg tcttcccaca gcccgattca
    16561 ccgaggatga ccacggcgcg attggaatcg aggtcaaagt caaccccatc cacggcttgc
    16621 acggagccat tggcggtttt gaaatgcaag acaaggttct tgatatgaag aagtttctca
    16681 ggcgcagcgt tcatacatcc ctcagttttg gattgaagac ccggtccagt gcgaaaccga
    16741 ggaaggcaaa ggccaacccg gtaatcatca ggagcatggc aggctccagg acccagtagt
    16801 aatacccttt gtacaatgcg ccattggaat tggcatcctg aataatcttg ccccaggtgg
    16861 gcagcacagg gtcacccagc ccgatcacgg ccagtgaagc ctctaaaaat acaaaggcgg
    16921 gcaccgattg aaccaaactt ggaatcaaca acgggatcat gcgcggaatc aaatacaaga
    16981 aaatgatacg cggactgctc gccccatacg ccttggccgc ttcgatgtac atcgattcct
    17041 ttacctgaat gaagatggcg cgatatgcct tgatggaccc tgtgaagatg ctcaagataa
    17101 tcgtcgcacc gaggatgacc cagatactgc gcgaatagaa agtgccgatc atgatgagca
    17161 gcgcatagaa tggcagcacc agattcactt ctgtgatgcg ttggatcaac tcatccaccc
    17221 agcccgcgta ccaggcaccg atcgcggcga tgaccatcgt caaaattgat gtgcccattg
    17281 aggcgatcaa cccgaaggcc aatgccacgg ggacacccca caacaatggc aggaccaggt
    17341 cacggcgcgc atgatccgtt cccgcccagc cgaagacctg tccatgtgaa acaaattcca
    17401 cattcaagtc agagtcaggt tcgaaggtcg tccctgtgat gatgagtttg tatttgcctt
    17461 tcaagggctg gtcgctgccg ggcagagaga acagggcggg gatgacctca tccgcacgta
    17521 atttggtcag cagtttttca tcctgcgaga agcggtaggt gaactcattg ctgacagaga
    17581 agtttccaat gcggatggta cgttcatcgg gcgtgagcaa ttcaacagag ataaaaggtt
    17641 gtttttcctt gtatgtggtc gtgacgtata aaatcagatc ctgcggatac acgtcgtagg
    17701 aataatcaaa ctcataggcc atatcgaagg ttgaaatgcc ttcattgccc ggggtgacgg
    17761 tcttcgtcat ctggccgtct ttggtgttga cggcaaacga ttcggaatat tgtttgctgg
    17821 aaaaataatt gatccaggca ggcgcggcga accgcggatt ttgataccag acctcctccc
    17881 caccgcgcca caaacgtacg gcctcggtat agggaatggt gatcatggca taggcggcaa
    17941 caaacaccag ggcaaacact acaaaaatgc ccgccactgc cgatggatat tgaaccagtt
    18001 tctggaacga acttctaaaa ttattcatat tcctcagccg cctatcttca cccgtggatc
    18061 aaccagggcg taaataaaat caagcagaaa aacggtcatg gcaagcatgt aggcaaagat
    18121 gatggatgag ccgacgatga tgggcgtatc gtattgaccg atggcttgaa aaagcgtacg
    18181 acccagcccc ggccagagga atacagtttc tgtgaaaagg gcacccgtcc acagggtgat
    18241 gatgagcagg gaaaagttgg tgataatggt cggcagtgtg gggcgcagga tgtaggtccg
    18301 ctcgatatcg cgggtgggca ggccctttgc ctttgccata tccacataat cttcgctgga
    18361 gtagatgagg aaaaaggtgc ggtagttgta aacgctcaaa aagaacgaac ttagaatcag
    18421 cgccaggcaa ggcagggtca agtgcttgag cacactcatt gaataatcaa aagtgttgtc
    18481 aggcgggggc gaatccacca tgccgccaaa gggcagcact ttcaaagccg ctgcaaagat
    18541 caggatcaga aagatgccat aaaaccaggg cggtacggcg gacgtaggtg aaagcgcgat
    18601 gaccagctta tccaggaaac tgccgtactt gcgcgaaagc gaaagggcaa taaagatgct
    18661 ggaaaagaac aggaataatt gcgaggtacc catcaacaac agggtggcag gcagccgttc
    18721 gagcaggatc aaacgaacct gtttggagcc gctatcgctc gtcagattga tggcgcggcc
    18781 aaggtccaat tgcagggcat tcgagagata acggaagtta cgcactgcaa tgggggtgtc
    18841 caatccgcgg cgtttttctt cgagggcgat ctggtcttcg atcaatttct tgcgcacatc
    18901 gggagccatt tgcaaggcgg ttggattatt cgcgattctt tgcgtcaccg aatcgcggat
    18961 ctgtcctttg atgatctcat ccacaaaacc gcccatgttg gcgatcaaga tggtcaggta
    19021 gattccgatc accacggtga tgaaaagcgt caacaaacgc ttcgctgaat aacgcaggat
    19081 gcgtgcaaag gaactgttga gaaatgattt tttgacgacc acattctcca aaggttgagc
    19141 ggtagctgac atgactgcct ccacaatcga gggttcaggg cggagcgggt gccccgccct
    19201 gaacaaatca cttacttgat gacaacgaaa tcgaacgatg tgaaggatgg gatcgccaca
    19261 ggaatcggga cgacagccac ttccaatttc gctgaaccag tttgcagttt ggatgttgct
    19321 tccgcatcca acgtcacctg gaactgcccc tcgccaacgg cctgcgcctc cccgacggag
    19381 accaccgcac cagtggtgtc gtagagaatg tacttcacca gcttgatatc ggcatttgcg
    19441 taggcggcat cgccaaggtt gaccgtcaca tcaaagacgg cttcctgacc agcagtcacc
    19501 tgcccggggc catcgaggat ggtgcttgcc agtttgggcg cgctaaagcg tgaccagcga
    19561 tcggaggcat ccacatactc ggcgttgtgt ttcaacacga gcgatttctc ggtcgtaaag
    19621 acctgatcga gatagtacgg tccggtgcca acccagaagt gaccatgatc ttcgtaccac
    19681 ttcttcatgt tggcgtaacg ctcggtgatc tcttcggcgg tgaggtattg acccagagtg
    19741 ggttcgtacg gaatcaggtt ttcagactgt gcctgatcca tgtacttctc aaggatttca
    19801 aggctcggcc cgcccaccca gctcatttgc tcgatctggg ctacatcggc tttgtcggag
    19861 gagtacgcga gttcaccagc ggcttcagca aggttcgaaa cagccaaaat ctgccagcta
    19921 ttttcaccgg aaagaccaaa gggagaattc ggccaaaggg tgaggatgtt caattccgca
    19981 tcggcattgt aggaatccga gtaggcttca atggtcaacg gatcggtcga aacgatgcgg
    20041 tatcccttga aggatggcag gaagcccgcc agaccaggga cagccgcttc atcgtaaaca
    20101 tagctttcgg gtttggcgcg gtcaaagaaa atgatgatcg acatgacaaa gtcggctaca
    20161 gagatggaac tgccatcatg ccatttcaca gtgtcataaa ggtcggcagg gaaggtgaca
    20221 acactcttca ctttggccgt ggtgccatcg gggaatttct cgcccgcggt gatgaatttc
    20281 tggttggcgg cgtcccaatc cacccaggca tcttcgggca catcaatttg gtccacagct
    20341 tccacgctca accagtcgag gttgtgggtg atgggcaggc ccttttgata ggtcaacgaa
    20401 gcgctttcga tgcgctgcgg ccaggccagg cccgtgtatg gatcgcccat gatgccgcca
    20461 gtgccggtga tgctatctgc gccctgcgta gtggctcgca tgacattcga atcccatacc
    20521 cagttactgc cgccgatggt gttccacggc tgggtgaaga gatcgttggt gccgatcttc
    20581 atggtgccac cctcttgatc tttgaagcgc aggttgtagt tccccataaa ggtcgattcg
    20641 tagccagcgg ccagatcgta ggtcacagaa acattgctgt tgaaaggtgc ataggtctgc
    20701 tggtcaatga cgaagacgaa gagagaatct tccagagaaa gccgcaaagc ctccgccatc
    20761 atcgcatcac gttcttcctt cgtagagaaa ttacccaaag ccaggtcatc gcccagtttc
    20821 tggaattcag ggtcagaaac gttggagagg aaaggctcgg cagcctgcac actggtgttc
    20881 aggtaatatt gctggaagtt ggcgcgctca tcgcgggtga gacctgaagg caggtaccca
    20941 gcggtgtaca gattccattg gccgtctttc gcatcggtgc cctgccagat cgggaaggct
    21001 tcggaagagg tcttgtactg acgatcaacg gtgaaaccaa tggcttccaa ttgattggaa
    21061 acataatcac ccatcggctg gcgtgtgcca tctccatcag agcggatcag gaaggccagt
    21121 gttatgggtg cgccgttgta ttgccacttg ccatcggcac ccaattcagc acccatcccg
    21181 accatttcgg cgctaatcac ttctttggcc tgatcgaggt tataggcata tttggtttcc
    21241 aatgcacggg cggtttccac gaggttggta tattccacca actgggtggt gatcgagaac
    21301 aatttcggca gagagccgcc gccataaatt tcctggttga tgtagttacg gtcgatcagc
    21361 cagttcatcg cttcgcggat cttgcggttc gagaacggat tcaacactgc ggtatcggta
    21421 aatacagcag ggttcatgct gatggcataa cttccgccga acgactgggt atagcccagc
    21481 ccggcttcct tgatggctgg gaaagcatca gaagcaaggg caaaggagta aaaatcaatt
    21541 gtccctgcct gcagctgcga gatcgcagaa tcagccgcaa ccacagaaaa atcaatttcg
    21601 tcgagccagc cgccccgtcg gtcagtggcg ggcgccgcag gcgcttccgt ggcagcggtg
    21661 gtgggaggtt cggttgcggc aggcgcatcg gtgggcgctg gttcttcaac tgcttcagta
    21721 gcagcgggtg cgcaggctgc caggatcatg ctaaaaacca caagcagact gaacaacgca
    21781 tataattttt tcagtttcat tctcttttct ccttggtttg gttttgaaca aatgaaattg
    21841 catacaatgg aagatctaca accaaccacc tcctttgtct tttattcagc agatagaatg
    21901 ggaggcggag ttcagtgcgt aattctgaaa tccgcctcgc ctgtcaaaga gcggtatgct
    21961 ttcgtgagga gaaggctgcc cgccaaactt gaactcatga tacgaattcc cgctgcaact
    22021 gtcatgcacc tgcataagca tctttgccaa tacacttgaa ccgttcgtca accatgccaa
    22081 accacccgca ccctgctccc aaggcaataa aaaaaccctc ccagaggagg gtattcaaac
    22141 aggtggggcg ggcccaaact gcatataatt gaactgacca tgcgaaaata cttcgtctac
    22201 gcgatttttt tactcaccct gctgggcaat tcctgtgttg cggcgcccga caccttgaag
    22261 cccccactgc ccgttgtatt cgacgagcca cagtcgggct tgaattcatc gcgcaacagc
    22321 cgctcaaccc catcctacag ccagaacatc cgcttcgaac atttcagcct tgaagaagga
    22381 ctttcgcaaa gcacgatcac ggtcatcttt caagacagca agggcttcct ttggatcggc
    22441 acggaagacg gattaaaccg ctacgatggc tatactttca aaatctacaa accaaacccg
    22501 gaagacccga ataccctcac agaccgctgg gtcacagcac tggcagaaga ccccgaaggc
    22561 aacctgtgga tcggcacacg cctcggcggt ttgaatcggt acgaccccga aacgggacaa
    22621 ttctcacatc tgctgtatag caacacgaac agcgcaggca tcagtcacaa tcacatcacc
    22681 gctcttagcg ccgataaaga tggcatctgg atcgggacaa aaaacggtct ggactacctc
    22741 tcatatggtt cacaaagaat cgagcatttc cgcgcctcat cagtcaaccc caacagcctg
    22801 agcggcaact ggatcagcgc cattattcaa gatgccaatg gcaacctgtg ggtcggcaca
    22861 caagactcag gcctgaactt ttacgataaa accaaaaaat cattttccgt tttcaaacac
    22921 agtgacgacg tctttaccag catcagcaac aaccatatca ccagcatttt gatcgacaac
    22981 cgtggagtgc tttgggttgg caccgccaac ggattaaaca actccgaatc ggcgggcaga
    23041 tacttcaccc actatgtcaa tgtcgatggc gactcggaca gcctgagcgg tgacgttgtt
    23101 tacgcgatct atcaagaccg ttccctcggc atctggatcg gaaccgacaa cggactcaac
    23161 cgctacgacc ctcagcgcga aagttttgtg catcacaacc atcagcccac cacacccaac
    23221 agccttgcaa acgatacggt gatttccatc tttgaagaca acagcaacgt gttatgggtc
    23281 ggcaccgatg gcggcggctt gaacaaatac aaccgtcagc aggaccattt tgcttattac
    23341 cgcgccaacc ccgatgaccc caccagcctg agcggaaact tcatcatgcc catcgttgtc
    23401 ggcgaaaatg gcactgtttg ggtgggcgcc tatggaacag ggctgaaccg cctcaatgcc
    23461 atttccaatc aattcaggcg atatgtgcat gaccccaaca actccaactc gatcagcacc
    23521 aacgatgtga ccgcccttaa aatagacagc agcggcaacc tgtggatcgg aacctccatc
    23581 gggttggatc gcctcaatgc ggatacaggt caattcactc attttgtccc cgatatcaac
    23641 aaccctgaga gcctctcggc ggcccccgtc aatgtcattt atgaagacca ctcgaacacc
    23701 atctgggtcg gcaccagccg tggcctcgac cagtttgatc cgcagacaca ggtctttatc
    23761 aaatacgata ccgaagataa cgacccaaac agtttcgaca acaatcagat caccagcatg
    23821 ctcgaagatg aaaatctgaa tttctggatc ggcaccttcg atgatggcat caaacgcttt
    23881 aatccgagca acggcgaaat caccagctac accaacaatc cagataatcc ctccagcctg
    23941 gggagcaacg ccgtcctatg cctgcacatt gaccgggcag gcaccttgtg gatcggaacc
    24001 gcaggcggtg gattaaaccg ctacaacccc gaaaccgact cgtttgtccg ttttaccgaa
    24061 aaagacggcc tgcctaatga tgtcatctat ggcattctcg aagacaaaaa tggttttttg
    24121 tgggccagca ccaatttcgg tatcacccgc tttgacccca agaccaacac cttccgtaat
    24181 ttcacggcca gcgacggcct gcaaagcaat gagtttcatc aaaatgcctt tgcaattgac
    24241 ctgaaaggca acctctactt cggcggtaac aacggcttta atgtctttac acccgatgcc
    24301 attgacagtg ataccgtggc gccaaagatc gtactgacct cactcacgcg cgaccgcacc
    24361 ccgatctcca acaacatccc ttctgaaagt atcaaggaga tcaccctgca atggccgcaa
    24421 aattcctttg aattcgaatt cgccgcgctg gcttatgagc agccttcgaa gaatcaatat
    24481 gcctacatcc tcgaaaactt cgaagccaat tggaacatga tcggcaccca acgcaatgga
    24541 cgctacacca acctgcccgg cggctcctac accctgcacc tgcttggcgc caacagcgat
    24601 ggggtctgga acaaggaagg cgtctccatc agggtcacgg tcatcccacc catctgggaa
    24661 acctggtggt tccgaagttc cctgctggtt tttctggcag catccgtatt tttaggatac
    24721 cgctggcgtg ttaaacgaat cgaagcacgc aaccgcgaat tggaacgtct cgtccaaagg
    24781 cgcaccggcg atcttgaaaa acgcacccgt gaaatcgaag ccctttatca agcggacgaa
    24841 cggattttac gtaacgtcac cctgattcag gttttccaaa ccctggtgga tgtatccgtt
    24901 tccatgctta aggcagaccg cagcatggtt ttcatttgga acgaggaaaa gaacaagatc
    24961 accccgcgcg tcagttatgg tttccgcact gaaacattgt atgcccttca tttcgaccag
    25021 ggcgaaggac tggtcggcca aaccatgaaa acaggcgaga cattgatcgt aatggatatc
    25081 aaacgcggca ccctgcgcgg cgatgtggaa accgtcattc gcagggaagg gatccaatca
    25141 tttgcccact tccccatcgt ggtagatggc aatgtcatcg ccatcttcaa tgtagcctac
    25201 acccgtccaa acgcattcac cgatgacacc atccgcctgt tcacatcgct ggtcaatcgc
    25261 gctgcattat ccatcgccaa catgcagctc ttcgaacaga ccaaggatct ggctgtgatg
    25321 gaagaacgta accgcctcgc acgggacctg catgatagtg ccaaacaaaa ggcatttgcc
    25381 gcccttgcgc agttgggtac cgccaatggc atcctgaaaa caaagccgaa tgatgtgaaa
    25441 gttcacctca ccgaagcaga gactcttgtt ttcgaagtca ttcaagaatt gaccttcctc
    25501 attcaagaaa tctacccgat cgccctgcag gaaaaaggat tgcccaccac attgcgtgaa
    25561 tacatctttg aatgggaaaa ccgcaacgat accgaagtca agctgacgat tcgcaacgaa
    25621 cgtccgctgc cgctggagat cgaacaggcc gtttaccgca tcattcagga agcactggcc
    25681 aatgtggccc gtcacagcaa ggccaaacgt gcagatgtat cgctggttta taattccgac
    25741 tccctgcaga tcatggtggc cgatgatggt cacggcttcg acatgaatca gcgggccaaa
    25801 ggcatgggct tccgttccat gcgcgaacgg atcagcagtg ttcgaggaac cattcaaatt
    25861 caaagcgcgc ccggccaagg gactcgtctt attgcacaat tacccatcaa atcaccgggt
    25921 aaggaggctt caacatgaaa caccatcgca cgagcatctt aattgcagat gaccatgaag
    25981 tggtccgaaa tgggatccgt tcctatctcg aaacactttc agattttcag gtcgtcggcg
    26041 aagcctcttc aggagaagag gccctgctca tggtcgcaga attgattccc gatatcgttc
    26101 tgcttgacct catcatgcca ggcatggacg ggatcgaaac cacccgccgc atcaaacaga
    26161 ccagcccgcg tacacaagtg gttgtgctga cctcctacca tgaggatgtg catatcttcc
    26221 ctgccttgaa ggccggggcc attgcctata ttttgaaaga catgaaaatg gagaaactct
    26281 ctgaagttct acatcgcgcc gtacagggtg aggtgaccct gcatccgcgg gtggcggcac
    26341 gtgttcttca gaacattcgc ggtgaaaatg gagaggagca gcccctgttt accgatttga
    26401 ccgaccgcga aaccgatgta ctgaaactca tcgccaacgg cctgaccaac agccagatcg
    26461 ctgaaaaact tgtcatcagt gaaaacaccg tcaaggggca tgtgagcaac atcctaagca
    26521 agttacacct ggcagaccgt acaaaactgg cggtgtatgc ctggcagcaa ggcattgtca
    26581 atagagacag cgccccacgg gaataaaccc gcctgaaaca aaaaaggaca cggtctttcg
    26641 accgtgtcct tttttgtttt tagaaatcct attgacccgc tgcagccaaa gccgcatcga
    26701 tcgcttgcgc gaatacatca aatggctggg cgccttcgat cagttgggtc acttcttcgc
    26761 cgctggcgtt gacatagctc aagacgaagc caggggtacc gcgcacaccg gcggtcagcg
    26821 catccttgcc atcctggaag acctggtcgt caaacttgcc gctggaatag cattcattga
    26881 attgggtcat atccaccccg gtttgttcgg caatggcagc caaacggcgg tcggtaaagg
    26941 aatccacatc ttcaccaagc acattggcaa agagcatatc atgcatttcc cagtacagat
    27001 tttgatcgcc tgcacaatag gcagccttgg cagcatcttc agattcggtc ttgccgccgc
    27061 ggatgttgtc acttacccag ttccccgcgg aacgataggt aaagtacagc ttgccagtgg
    27121 aaacataagc ctcgaccagt tttgcttctg tctcagaagc gaatcgttca cagaacgggc
    27181 actgataatc ggaatattcc acgagtttga ccggagcatt tgggtcaccc atgttgttgg
    27241 catcagcttg cgggcgcgga tttggctcaa cagagataat gccagtaatc ggtttcaaat
    27301 taggccaaat gatcaaaaaa gccacagcca aagcaccaag tacgaccaag ccgatgccga
    27361 taaaacgctg gcgctgagac tgacggcgta tctgctcacg gcgcgcctgc cgtttgctca
    27421 attctttcgg gttattcata ttctcttact ctcctattgt ttagatggat gcgaattttc
    27481 ccatgctgaa atcaatttgt ccagtacagg gttaatgggc tcttattttc actcacgcag
    27541 gcgggcatcc atccacagcg ccatggtttt gaaaacttcg gccttttcag gctcgttgtg
    27601 cagttcgtga tacgcgccat cccacaggac gagggtgcag cgttctttca gcggggcggc
    27661 gaattctgaa ctgccagcgg aaaaggttag gtgatcctcc ttgccatgca tgagcaggag
    27721 gggcaatggg aattcactgg catgtgccag ggtccagctt gtaacgccga tcatgatctt
    27781 tccaaaccca aaagacatct gatcatgcac caacgggtcg ttgatatagg cctgaattac
    27841 tttttcatcg cgggagatgg ttttcggatc gagtccactg ggaatggtca cagatggggc
    27901 cacagacccc agcacctttg ccaacatcac cttgaccttt tgatcctcga gggcggtacg
    27961 cagaccagaa ctggtggcga tcacaccctt gatattgggc tttcgcagca gggtgtagtg
    28021 caaaaccagc accccgccca ggctgtgacc atacaaaatg atgggaaggt tggggtagcg
    28081 gttgcgggcc tgttccagca gctgatcgat gacagtcata aaatcttcga tggagttgat
    28141 atgtccacgt tttccaccag aacggccatg tccgcgcagg tctgggccaa acaggatcaa
    28201 cccttccctg ctaaaagcct ctgccacatg agcataacgc gaggtatgct ctccaagacc
    28261 atgaacaaga caaaccacgc ccttgggctg cagcacggtt ggttcccaac cctgtgcaaa
    28321 aacttccaaa ccatcatgag ctttccagct cgtttcaaag tgcttcacgc ttctcctcca
    28381 atgctgatct tgaattcaca ttgccgtgcg cccatggctc gacaggcgcg ttcttcaata
    28441 tcgtgttcct gccctaccgc ccaaaacaaa cattcacgga tcaggccgag ggtaacaaaa
    28501 cagatcgggg ctgtatcggt ttgctccatc gccgtggggg atgcatggtc aacaaagagc
    28561 aggtcaaggt ccagagaatg aaccgagaca tttcccgatg tggcgcttaa gatccgtgcc
    28621 agcagttcca aagcaggttt acgccgcaat ccttttggaa gcctttgtat aatggcggcc
    28681 tgcgctttaa ttccaaaggc ggaatcgttc aagagttgtt cccaaagttt taccccgatc
    28741 cataacaggg tgctgcgcgc accgcggcca taatacttgc gcaaagcaga ttgcaggcgt
    28801 gagtacacat tggcggcacg ctggtcatcc aatgcagaaa aatgcgccga atgcgcccac
    28861 tctgcaggca ggcctgcatt cgtcaatgca ccggtcaatg tttcgtgacc catttctgca
    28921 gaaagggttt ctacgaatct tttcaagata tgaacgggga aaccgatctc tgtcatcagg
    28981 cacctatccc aatgggtaaa ccaacactgg aaaatattca ccagtgcgga cgaagcccat
    29041 ctttttgtaa agtgcaagtg aagcttcgtt atctgattgt gtgttgacgg aaagacgatg
    29101 caattgggca ccgccaaacg attggatcag gtcgctgacg agggcagccc ccacccctct
    29161 gccttgcgct tcaggactca ccccaagcct ggccaggtgt gccccaagcg gattcctcgt
    29221 actgagttgg tacccgatca cccctgaatc atcttcagcg accgtggcac ttgcgcattg
    29281 caatcgtgcc cgttgaaggg tgcttgctgt gttgtgccag aaatctccga aagcggccag
    29341 atccaatttg gcgaccatgg gaatatcggc atcggtcatc ggtctaatac gaaccccgtg
    29401 cggggttaga aagtatcgaa tgtcttcgtt tcgcaattca agcaatacga tgttctgttg
    29461 attgataaat ccactggaaa gcaaaagcgg ttgaaaccat tggcggacaa cgattgcggc
    29521 aaccttgagc ggcctgttct ccgatgcaag atcactgcgg gctttttccc aaagcaatga
    29581 ccacgcttct gccccagaga gggaggggct gtatccaaaa aaactgaccc aggccacgtt
    29641 aggcgggtct tcagggcaaa ttaaaacagc atggatgctt gcaccttcag caagcaccca
    29701 gcaattcggc aggttgatcc gttccagcgg tgaatgccaa tccaaatggc ggtggttgtt
    29761 ggcttcgtag tagacaaggc tggagacctg gttgtaatct tcagcaacca tgcggcgggc
    29821 ctgcaagttg atattgatca tttgataaag cggagaaaga atttaagaat gcgttcgaag
    29881 aagccaggat tgtccttggc atcaagagac cccatcattt tgaaggccga ttctgttacc
    29941 ttgcctgttt tgagcttgat tgccgcggca gatttcatga ccatggcacg gaggtcgccc
    30001 tccccaactt cggcaaataa ttctgcagaa cgttcatagg cggcgatggc ttcgtcaaaa
    30061 cgattgagcg cttcgagggc ggcggcttga ttccccaatg ccattccctg acttttaatg
    30121 tcctttgccc ctgcaaagac caaatcggtt cccaatgcgg catccagcga ctcttgcgct
    30181 ttgcccgcct gcaacaatgc cacactcatg ttgtttttca tttcagcggc gagcagccca
    30241 ttgcggccaa gtgaaaaccc ctccgcggcc tggcggaaca attcggcgga ttcgtcaaat
    30301 tgtttgtttc tgaaggcctg cttgccttgt tctgcgagtt gggcgggttc gattgtcatg
    30361 gtcatttccc aaaatcaaga atgttttgca acgatgcagg gaatggtaat ccgctaaagc
    30421 gtgatatcca agagcccttc ggcaacagag cgcaattttt ccttggacat gtcgctgagt
    30481 ttcatggcca attccaggta ggggcggttg accggctgac agacaaaatt ttgaatctct
    30541 tctggcaatt ccaaaaactt ttgcatggcc cgctgactgg tcatccactg gcctacgggg
    30601 ccattctggt caaagaaggt ttcaatgcgg ctgcccaaaa ccgccagaat gctctccaac
    30661 tcagggacgg agatcgggcg ttctcccaat tcatacaggt tcagccgtgc ggatttgatc
    30721 cccgtatcca ctgcaagctg ccgaagcgac atattgatct tgttacgttc ctgtcgcaac
    30781 aaggcaccga tcattctttg acgtaatgcg atcaactgtg ccgtatcaag agtctccgcc
    30841 ggggaggatg tctcggacat ggtttcccgt ccccagaagt gagtgatcgg caacttcaga
    30901 taataaacaa gcgtttccaa ctccggcagg gatggggaac ggcgcccttc ttcataagca
    30961 cggaataacc ccggctttac accgatggca tccgcacatt ccttgatgct gcgtcgttcg
    31021 gccatacgcg catcacgaat gagtaaacca agcttttttt cacgtatgat gatctgggcg
    31081 ttattcatta ttcctcgtaa atagggttac aggatatttt ttaatttggg tcaagggtca
    31141 ttttcgccag caatgctcaa cgtggaattt attttgctgc atggcgggcg aacaggccta
    31201 gattatagca gagtgtttca gcgccgagag ccctgattgg ccgacgcttt tggcgcaaag
    31261 atatcagcag tgagcttgaa cccagccgct tcaaggcggg gtgtgaagtt gggacgaatg
    31321 cctgtggcac tgatctcgcc gagcactttt ccttcggtgg tcacgccggt ttgaataaac
    31381 ttgaacacat cggtcaacac gatgatctca ccttccatac ctgccacttc agtgaccgct
    31441 gtcaccttgc gctgcccatc ctttaagcgc gtctgttgaa cgatgagatc aacagcagat
    31501 gaaatttgct ggcgcaccac tttaattggc aggtccatgc ctgccatgag caccaaggtt
    31561 tcaatgcgcg agatggcatc ccgcggggtg tttgcatgta cggtggtgag tgaaccgtcg
    31621 tggccggtgt tcatggcttg caacatatcc agggcctcgc cgccacgcac ctcccccacc
    31681 acgatccggt cggggcgcat acgcagggag tttttcacca gttcacgaat ggtcaccgca
    31741 tgaagtcctt ctgcattggc tacctttgtt tccatccgca tgacatggtc ctgctggagt
    31801 tgtagttcag cggcgtcttc aatggtgatg atacgttcgt tttcgggaat ataccccgaa
    31861 agcacattca acaatgtggt tttgccagac cccgttccac cagaaatgat gatgttgaag
    31921 cgggctttca cacaggcttg caaaaaatca gccatttggc gggtcaatga accgaaattg
    31981 atcagatcct cgggctttaa cttgtctttc ctgaatttac gaatggtaat ggaaggccca
    32041 tcgatggcca ccgggcgcac cactgcattc acacgcgacc catcgggcat gcgggcatcc
    32101 acggtgggag agtctgcgtc tacatggcgg ccaagcggca ggatgatacg atcgatgata
    32161 cgcatgacgt ggtcatcgtc atcgaaggtg accgtggact tggtcaactg tcccttcttt
    32221 tcaacgaaca ctttcttggg gccattgacc atgatctcgg aaacatcctc atcatccaac
    32281 agggattgga tgggaccata tccgagcacg tcgttcaata cctgttcaaa gatctgtttt
    32341 cgcagatcct ctggcagttt caattgcgtc tgttcataca catggacaat gttctttttg
    32401 acaaaggcag agcgttcagc agcgggaacc cgctcggaac ccattgcact catcaagtta
    32461 tcggcgagat atttctttaa gcgggagaga tcctgtccgg tcaatctgct ggatggggta
    32521 acggttgtca tgatcaacct ccctggctgg attcatctgc gggggtgatc catatgatct
    32581 gtgcgcgcaa aatcgccaga aacgccccgg agaaaagcac cttctcgttt gcatcctgaa
    32641 tagtgaaatt ggttacggca ataaaccttt catccatttc caattcatct ttgagccttt
    32701 ccccctgacg gacatgtacc agcccacgta tcaggtgtgt ggtggtttgt atcacaactg
    32761 cggtggggac tttggtcacc acttcggtgt agaacttgcc cttctcatcg tattcaattg
    32821 tcatgatgct tttccttgtg accgcccgca atgcaagcgg tgataaaaag aggctttggt
    32881 gctcgcctta catggatgca ggtgtaacgg atgtacacct gtatgatcga tgaagcacgt
    32941 ctattataag tctattgacc tgttcgtagt ttttgagcaa gaaccgccat ttccttcgcg
    33001 gtctccaaaa agacaaagga agccgtatcg ttcgggaatt ttaaagaaaa aggttgatga
    33061 tggctggagg cgaacgcgaa actgctgcca aggtagggca tggccgcttt gatctgaata
    33121 tccaatgcct tttcagcatc gggcttgctc aatccctcca atccgctggc tcggttcaag
    33181 atcggaaaga tcgtggcagg ctccacaccc ttggttttca tataatccaa cacggttttt
    33241 gtgagcggca gggcactgac atcggtactg atgatcaggg cgaccagatc ggcgtgctga
    33301 aggatcggaa ggctgatctt cgaaagtgcg cggccgatat cgatcaacac ataatcatag
    33361 gaagctttca aagcagcgat cacgttccag atgcgcccca ctttgagatg attgctgctt
    33421 tcagggtcag gcgaacccga aagcagatga aaacgccaga tgccagcctc tttcaattga
    33481 tcgcggaaat aatcgggggt gatcttttct tcgggcatat ctgaagctgt gacgatgttc
    33541 aacggtcctt catagccgac aatggatcca atggacccaa tgggcagaac caaatccacc
    33601 acagccacac gggcttcggg ttgattttgg cagacggtca aaccgagatt tgcacacaac
    33661 gaagaagttc caactccacc cttggcgctg ataaaggcca ttaacaaacc gccttccttt
    33721 acaagcgcgg acgatattcc cagcaggcgg ttaatcgcat cggtgagggt ggccatggcc
    33781 tggcctgatt ttgcgatgta ttcgctaaac cccgcatcaa ggcacgactt gatgcgggcc
    33841 acccccagat cactgctcaa tgcaatgagg ggcatattcg tggtgcgggc atcctgcttg
    33901 agttttctgg caacctcttc cccggtaata tccgggacgg tggggtctat taccatcaag
    33961 tcaggacgat cccgccatgc atggatcaat ccctccttac ctgaaccagt ctgaatgatt
    34021 tcatattttt gagcctgcag attacgtgaa ataaaattac gactggcgac atctgcatca
    34081 acaataagga tcttcttttc agacatgatt caaattcctt tgcgcgtgtg ggtcacgcgc
    34141 accgccgggt caaatttctg tgggcggcat caagtatccc agaccggagc gggtcacaat
    34201 gtggcgcgga gaatgcgcat ccgcctccag tttctggcgt aaacgggcga tactgaccca
    34261 taagatttcc ttgtcgttct tgtactccac accccataca ctggttaaca ggtcttccga
    34321 ggtgaggatc tttcctacgt tatgcgtaaa ttgcagaaga aggcggtatt ctgtggcgga
    34381 aagcgaaact ggttcttcac cgcgccatac ttcggcacgt gcaaaatcga tcttgaggtc
    34441 atcatgtgta aagaagcggg cctgccccga ttcggtgggc gcttgtgccc ggcgtaaaac
    34501 agcccgcaca cgtgccagca attcggtggc agagaaaggc ttcaccaaat aatcatctgc
    34561 accaaggtcc aacccacgga cacggtcctg ctcttcgccg cgtgccgtca aaatcacaat
    34621 tgggacgttc gagaattccc gcaacctttg gcatacccca aacccatcca accgaggcat
    34681 catcacatcc aaaagcacaa ggtcaatggg ttgtactgaa aacacatcca aagcctgcac
    34741 accatcctgc gctgtggcta cttcataacc ttcagtacgc aggtttgcct ccaacaggcg
    34801 caaataacgg ggctcatcgt caacgatcaa tatacgcttg gacataacga tctccttgag
    34861 gaaagagttg aaggttggtc aaagcgttga atcgacaggc agttcaataa tgaacgttgt
    34921 gcctttatcc agctctgact ctacccaaat attaccatga tgcgccataa caatttgctt
    34981 gcaaatatac aaccccaaac cagttcccgt cacggtacgc tcaccgggca cacgatagaa
    35041 acgttcaaaa agaaagggga gataatcttc cgggatgccc tcacctttat cggcaaaaga
    35101 gacgcggatc ttatctccct gcatgcgcat ggcgatcgta atttcggaat tgggcgcata
    35161 tttcaaggcg ttgctaaaca gattttcaaa cacctgtgca atgcgcattc catcaccgcg
    35221 aatggagggc agtgaatcgg tttcaaaatt gaccttcaat tcagggtgat gggtatggat
    35281 gcgcatggct acgtcacgag caagcgcatc gattcggatg ggtgaaaatt taaattgcag
    35341 ggtcttgctt tgcaggcgtg ccgactcgag catgttctca atcaatcgtg ccaggcggtc
    35401 ggcttcttca tcaatgatgt tgagaaactc gcgctgagtg gtttcatccc aggtggtatc
    35461 ctgcctgagc aggctggtgc tgtaaccttt gatgaacccg agcggggtgc gaagttcatg
    35521 tgaaatggtg gagacaaaat catcctgcaa acgcatctgc ctttgcactg aatccagttc
    35581 ggtgcgcgcc tcctgcaggt ctctgcgttc gatcagggcc gtggcccaaa acgactgcaa
    35641 agatgcgatc tgaatatgca ggttggaata ttcagggccg ccaaaacgga tgaagaccag
    35701 agcgccattc aattttccgc ccacgtacat ggggaaaccc aacaaatgag cctcggtcag
    35761 gcggttggca taattgagat tctttctcgg ctccttgagg atcattctgc cttttgaaag
    35821 cacctcattt gcaatgccct cgccccacat tgagtctgct tcggcggctt tgccgcgccc
    35881 cactgcacgg gcatacacaa cttcgagacc cttcgtgcgc gggtcttcca gatagatggc
    35941 ggcattgtca aatacaaaag aaccgcgcat ggatacaaac aatgcatcca acgcggctct
    36001 ccaatcctgt tgtatctgaa cctcttgaaa ggtcttgtgc aaaaatgtta gcgtagccag
    36061 atccatattt agtcacccag ggcaaggagc gggaatttga aaacagagcc tttgccttct
    36121 gctgattgta catccaataa cgatccgtgg gcttcaacga tctgccgcac cagtgaaagc
    36181 cccaaccccg tgccgccgaa atgccgcgtt gaagaaccat ccaattgatg gaagggttcg
    36241 aagatctctt ttattttatt ggatggaatt ccaatgcctg tatcggtgac cgtaaggaca
    36301 acgagattgt gaccttcctc tttgagggag atcaccacgc ttccacctga cggggtgaat
    36361 ttgatcccgt tatcgaggag ttgactcacc acccagccga tcttctcagg gtcggcctgc
    36421 acagatggaa tattctccaa agctgagacc agcacctgta caccgcgttc ctcggctttg
    36481 ggcagggccg atttcgcagc cagcgaaacg atgcggcgga tatccactct ttgcagtttc
    36541 atgctcatct cgcccttcga agccagcgag aacatgatga ggtcttcgat catgccttcc
    36601 agttttgccg tggcccgttg actcacctgc agggcatggc gctgttcttc tgagatttca
    36661 cccagcgacc cagccaccag caattcgata taacctttta catgagtgag cggtgtgcgc
    36721 aattcatgtg aaatgttcga aacaaaattc gatttcaact ggctgagttc cgaaagcctt
    36781 tgcagggctt cattcaattc tgccgtgcgt tccttcacgc gctgttcgag gttgcgattg
    36841 gccgactgca aagccgagcg caattgcagg atctgctcag tgaccacctg atcgagcgtg
    36901 tcacgatcca gcaggtttag atccatcaaa gactggccga gcagataatg attgcctttt
    36961 gcgatctggt cctgctgata cgtgagcgcc cgttgcaggt ctgcttcggt gatcagccct
    37021 ttctggacga tgtactcgcc catacgggga atcagcattt cgggggtaag tttcggaagg
    37081 ttttgtgaca tggggtttct cggtaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37201 nnnnnntcag catttcgggg gtaagtttcg gaaggttttg tgacatgggg tttctcggta
    37261 aaaatatact gccgtagtgc aaggtttacc ttgcgggtga tgtgggcaag gccatcaatc
    37321 ttccagcacc cattcaccgt ttatcatcgt tgattttgct ttaattgtca acagagattg
    37381 cgggtcggcg gagaagatgt tttcttcgag cacgatcagg tcggcgaggt agccttgtgc
    37441 aagtttcccg aggcggttct ctgcattggc ggcgtaggcg gcgccgacgg tgtaggcgga
    37501 taatgcttca gccaaagtca atttttgttc ggggtaccag ccttcggcgg agggggatcc
    37561 gtcggctctt cttcgagtga ctgcggcgtg caatcccaaa aatggatttg gggattcgac
    37621 cggggcatcc gatccaaatg cgagggttgc accatgattc agttgtgtac gccaggcgta
    37681 ggcatgtgcg gagcgatctc cccagaagcg gtcggcggcg aacatatcgg agggtgcgtg
    37741 aatgggctgc atggatgcga tgacgttgag ttgggcaaaa cgcggcgcgt catcggggtg
    37801 aatgacttgc acgtgttcga tgcggtggcg cagatgcggc agtccttttt ctgtttcgta
    37861 ggcgcgcagt tgttgatagg cgttgagcac ttcgtgattg gcgcggtcac cgatggcatg
    37921 gacggtcatg ctcaggccga catcggcggc cttgcggcag tgctcgaaca attcctcgcc
    37981 gtccatgttg aggatgccgc gattttcggg ttcatcctga tacggttgga acatggcagc
    38041 ggtatgcgga ccgagtgcgc catccataaa agctttgaca gagccgatcc acagccagtc
    38101 atcgccgaag ccggtgcgca ggccaagggc attcgcatgg tccacacttt cgacggggat
    38161 gtttttgttc acgcgcaatt ttagtttctt ttgcgcatgt aagtgttgca aagccatgaa
    38221 agaatcacgg cggtcaaaat catgaacgcc tgtgaggccc atcttccata aaatggattg
    38281 ggcgttctca attgcagatt cgatttcgga aatggtcggt tcgggtatgg tcttttccac
    38341 caggctcatc gctgtttcga gcaggatgcc cgttgcgcgg cctgtgttgt cgcgttgaat
    38401 ggctccgttt ttggggtcgg gggtatcgtt ggtgatgctg gctcgctgca atgcggcggt
    38461 gttcgcccag cccatgtgca gtgattttgc tgtgagataa acggggttgt tcggggcaat
    38521 ggcatcgagt tcggaggcgg cagggaaggc gttgtcatcg gtccattcat tttgattcca
    38581 gccgtgaccg aggatccacg cgccgggttt ggctttttgg gcgcgctctg ccacacggcg
    38641 cagacattct gccttggtct ttgtttcgca atctaccttt tgaagggaaa gcccgtagtg
    38701 ttgcagatgc agatgcgcat cggtgagtcc cggcagaatg gttctgccgc ccatatcctg
    38761 tttttccacc ctgccatgcg cgatggcttc aagcgtatct ttctccccta ccgccacaat
    38821 gcgcccgcct tcgatcaaaa tggcggaggc ctgcgggtgc gatgaatcga gtgtgtgaat
    38881 atttgcgttg tagaggagtt tcatgggatg tacctttgat ttttgattct atcatacgca
    38941 tcatgacaaa atttgaagag atcttttcag caatcacctt ccttttcctt aattcggtct
    39001 tcacacacgt t
//
LOCUS       DAOQLC010000025        38447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_305_length_38447_cov_27.445926, whole genome shotgun sequence.
ACCESSION   DAOQLC010000025 DAOQLC010000000
VERSION     DAOQLC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 38447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_305_length_38447_cov_27.445926"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PL000_06360"
     CDS             complement(<1..232)
                     /locus_tag="PL000_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC88554.1"
                     /translation="MLKIYNTLTRKTEEFQTLEPNLVKMYVCGVTVYNDAHVGHAMSA
                     LVFDIIRRYLEYRGYAVKHVMNYTDVDDKIINR"
     gene            complement(345..1430)
                     /locus_tag="PL000_06365"
     CDS             complement(345..1430)
                     /locus_tag="PL000_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNC88555.1"
                     /translation="MSFEFILRILAMFALAIGGGMWGFNFTKANPETDPIVTTTLSTV
                     VGALAGIILTPYFTTRPARYLRSLLGRLAAESLFAALTGLIVGLLIAALLGFPLSLLP
                     EPFGQILPFLGVLVFSYLGISLFTMRQGDIMGLLSALSGRGEGGGSSSWTNLNRNILL
                     DTSVIIDGRVADIAKTGFLPGTLLIPRFVLNELQYIADSPDGMRRQRGRRGMEVLSEL
                     QKLPNVLVRISDINVEGVREVDDKLIVLGKQLKSPVLTNDYNLNRIAELQGVTVLNIN
                     ELANAVKSVVLPGETLRINIMQEGKEYGQGVGYMDDGTMVVVENGKDYIGEYMEVNIT
                     KVLQTAAGRMIFGRVDEENSGKKGKGK"
     gene            1544..2524
                     /gene="moaA"
                     /locus_tag="PL000_06370"
     CDS             1544..2524
                     /gene="moaA"
                     /locus_tag="PL000_06370"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNC88556.1"
                     /translation="MAIDRFGRNIHYLRISLTDHCNLRCVYCMPEDMTFRPNADLMQD
                     DEILTFARLFASLGFDKIRLTGGEPTVRANIVDIVRGISSTEGIRSVSMTTNGLLLKK
                     LAQPLADAGLQRVNISIDTLNPNKFKRLTRWGKVEEVWEGILAAERAGLTPVKLNAVV
                     VKGYNEEDVIDLAALTIDHPWQMRFIEMMPFGGATDLQINQVITMKQIQDNIESEFGK
                     LESANDGKLDGEAQVFHIRGAKGNVGFISSVTHPFCSACTRARLTSDGKLRLCLLREK
                     EVDLLTPMRAGASLEELRRLLLDGVWEKPWGHGLADGVIPLNRVMSEIGG"
     gene            2547..3239
                     /locus_tag="PL000_06375"
     CDS             2547..3239
                     /locus_tag="PL000_06375"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC88557.1"
                     /translation="MFDLDGTLRHHLPSGGEVFVEFVRSMGFKVSEEDRIRAEHWEHF
                     YFASSLEIRKDSETYKDDRSGFWVNFGRRRLIALGLKASDALEVAPKASAHMNEAYKP
                     QVHVPPDAHTQLTALKEAGYILGVVSNRETPYQDELKELKLDSYFKFSLAGGEVNSYK
                     PDKIIFERALELAGTSAAETMYIGDNYFADVMGAHRSGLKPVLYDPTNLFPDTGGFAF
                     IRSFAELPELLK"
     gene            3337..4686
                     /locus_tag="PL000_06380"
     CDS             3337..4686
                     /locus_tag="PL000_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNC88558.1"
                     /translation="MAWERKLIRTVRVRNSQKTGVLARLLTAIAEAGGNTGSLVLLTE
                     TSQNVVRDITITAESEAHFETILDAMRSNEGTRIIEIRDEVLELHQKGKIAIRSRYPI
                     ESLTTLRRVYTPGVAEVCRKIAKDPSQARLYTSISHMVAIVTDGTAVLGLGDIGPLAG
                     MPVMEGKAMLMETLVGLSGIPILLNTKDTEEIISTVAAISPTFAAIQLEDISAPRCFE
                     IEERLQAMLDIPVLHDDQHGTAVVCTAALLVATRETGTDLNKAVIGQIGLGAAGHAIG
                     VMMMRLTGNPVYGADLSPEALERFEKAGGKKSNLREVMEKCDIVIATTGAPNLIKPEM
                     VRKGQIILALSNPNPEIDPEVAMEHGAAFAADGKSVNNVLGFPGIFRGAVDANAPRIT
                     HEMLLAAAHVIAEMTPPGELVPSPLDKKVHRAVARAVAETAIKQGIARAEYVPYVEE"
     gene            complement(4759..5988)
                     /locus_tag="PL000_06385"
     CDS             complement(4759..5988)
                     /locus_tag="PL000_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNC88559.1"
                     /translation="MQTPWEYRYAHRTQKMGSSVIRELLKFTEQPDIISFAGGLPAPE
                     VFPLKEFQEACNQVLTDHGPQALQYSTTEGYLPLREMIARHNARYSVRVTSDNIMITS
                     GSQQALDFIGRLFINRGDYVVVESPTYLGALQAWNAYGAQYISVPSDEDGMIVDQLEE
                     ALRIGPKFIYVLPNFQNPSGSTMSLERRKRLVLLADQYGVPIVEDDPYGQLRYDGEHI
                     PSVATLDSRYRNEDNGEYTGNVIYLSTFSKLLAPGLRLAWVIAPPQVIRRLVMTKQAA
                     DLHTSSFNQHVAYEVAKGGFLDEHVKVIRATYKERRDVMLELMDELFPSDVRWTKPQG
                     GMFLWGMMPENVDAAEVLKVAIDRKVAFVPGAAFHPNGGGKNTMRLNFSFSSPENIRE
                     GISRLALTLKEALKNGK"
     gene            complement(6163..6582)
                     /locus_tag="PL000_06390"
     CDS             complement(6163..6582)
                     /locus_tag="PL000_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC88560.1"
                     /translation="MADQIFPEPTVGVFIFNKKGELLLLQSHKFPGKYVVPGGHVELG
                     ERLEEAAIREAKEETGLDIYDLKFINFQQFIYDPSFWKRRHFIFFDYACRTDSESVQL
                     NDEAEDHVWVSPSEALHLPLDSYTRTSVEHIIAGNLS"
     gene            complement(6614..7468)
                     /locus_tag="PL000_06395"
     CDS             complement(6614..7468)
                     /locus_tag="PL000_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC88561.1"
                     /translation="MPILALVLLFFSAAMHATWNFLLKSAEEKYIAMGWQVILSGVFS
                     TVLIFFTGLPPRSMWTFAVISMILEAIYFILLCIAYSDHDFSLVYPIARGAAPALLVL
                     WTALFLHEQLTTGGYIGLAMITGGMILIGATTLLQNHEEKPHLRGILTALSVALIISI
                     YTYIDGNAVKHGPALPYGLSMFVMVPFVTTPYLVQRYGWSHFSAMWRQKRGYLLMGGL
                     LGLVAYMLALFAYTFAPLSYSGAIREVSAVIGAFLGWRFLKEEMGGIRVVGSAIVFAG
                     VMVIAVFG"
     gene            complement(7472..8335)
                     /locus_tag="PL000_06400"
     CDS             complement(7472..8335)
                     /locus_tag="PL000_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88562.1"
                     /translation="MQSQFVKTNSINLHVMTDGPENGTPVILLHGFPEFSYGWRKQIP
                     ALTAAGFRVIVPDQRGYNLSDKPKGVSSYAVDVLARDVLGLFDHFGIEKAFLVGHDWG
                     AVVAWTVALNHPERLKKLAILNVPHPDVMTEFVLKNAEQRKKSWYVFFFQIPLFVEWM
                     LSKNDFHNLGRMLVGSGRKSTFTLQDLAEYKKAWAQPGALTGMLNWYRAVFRRSLWHV
                     FGKKISARRITIPTLMLWGKRDVALSHEMVAPSIALCDDGKTVLFEKSTHWVQHDEAD
                     EVNKNLIEFLR"
     gene            complement(8340..9011)
                     /locus_tag="PL000_06405"
     CDS             complement(8340..9011)
                     /locus_tag="PL000_06405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC88563.1"
                     /translation="MKTIVITGATGVLGNLVAKTFAADGHNIALLDKDETKLESLVRE
                     LNLPSERLHAQTIDLLNGQAVQDSAEAVRSKFGSVHALIHLVGGWTGGKTIPESNAAD
                     LDAMINQHVWTTYHLLKSFAPHIANSREAWGRVIAVSMPVTVNPSAKMALYAAAKSAQ
                     ESLVLTLAQEFKENGLTANVIHVKSIDVKNEGKGTSPAEIVSAMQYLFSEEANKVTGA
                     RIPLY"
     gene            complement(9066..9824)
                     /gene="ribA"
                     /locus_tag="PL000_06410"
     CDS             complement(9066..9824)
                     /gene="ribA"
                     /locus_tag="PL000_06410"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNC88564.1"
                     /translation="MTTFTHEKIQLLMEEDCCHECEGYGSDKICVHIEAIAELPSRFG
                     DFHIVAFSNNRDGKEHVAIIKGEVLGAEDVPVRLHSECLTGDVVGSLRCDCRDQLEAA
                     LQKIGEMDKGIVLYLRQEGRGIGLTNKIRAYSLQDQGLDTVEANLALGFRDDERDYAV
                     AAHMLHSLKIQSIRLMTNNPKKIAQLEQHGVKVNERLPHILPSNEHNRFYLETKAAKS
                     GHMIDFHGKPHLVEQSDPPIVAGMTEDQIQALYE"
     gene            complement(9858..10544)
                     /locus_tag="PL000_06415"
     CDS             complement(9858..10544)
                     /locus_tag="PL000_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC88565.1"
                     /translation="MNRPFTFINVAMTADGKIDTFERKGSAISSKRDKTRVDELRAAA
                     DGILVGGRTLLDEAPKLTVKSEALREGRVQRGLSPNPIKVGVVTRIANADIPPDSDFI
                     KAGPSRVVIFTTNQTSKDKLAELTACGVEVFVHDAPRVDLNSMMLTLKKIGVDHLMVE
                     GGGTMNFELMRLGLVDELMIFVAPMIFGGANAPTPADGVGLPRDVAMGLKLIDVEKWE
                     DGGVLLKYRL"
     gene            complement(10575..11345)
                     /gene="ygiD"
                     /locus_tag="PL000_06420"
     CDS             complement(10575..11345)
                     /gene="ygiD"
                     /locus_tag="PL000_06420"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HNC88566.1"
                     /translation="MTEKMPVLFVGHGSPMNAIEDNEFSRAWRDAGKALPKPRAILCV
                     SAHWVTRGTLVTAMEQPRMIYDMYGFPKELYEVKYPAPGSPDLAEQVRRIIKKTEVYA
                     DQEWGLDHGTWSVLNQMFPEANVPVIQMSLNANLSINDHYEVAKQLKELREEGVLIIS
                     SGNIVHNLSMVQFRDFAYDWATEFDTQVKNWILANDHDPIINFQKQGQAAQLAINSGE
                     HYVPLLYSLALKDDDDKVSFFAEKVWGGSVSMRSVRIG"
     gene            complement(11355..11777)
                     /locus_tag="PL000_06425"
     CDS             complement(11355..11777)
                     /locus_tag="PL000_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC88567.1"
                     /translation="MNTNQRFAISIHALTLLASSNDPLTSEMIASSVDTNPVVIRRTM
                     ASLREHGLVESKSGAKGGWRLLREPKEIGLCEVYRSLDEVNVLAVHSHPNRHCPIGGN
                     IGGVLQSVFADAQSEMEKALGNYTVADILKDVRRKANH"
     gene            complement(11881..13482)
                     /locus_tag="PL000_06430"
     CDS             complement(11881..13482)
                     /locus_tag="PL000_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HNC88568.1"
                     /translation="MNPIRSRLTRIASVVLLFSFLASCATPTPAPTPQVFAPFILATR
                     AATSPTITPFQPVAQTDTSVPLLPTSTPTQQPTVPPSATFTQFPTQTQVPPTNPPPAV
                     TFTSPPPSAPSRTNYILYATMDFASRTLDVEETIRYYNGTGLTLSDIVLSVQPNLYGG
                     AFVLNTISQDSVALTTYTLNGQALTMNLPQPLPAGSATTLTLSFSLNVPAKGAGLFGY
                     DFNQINLVDWYPFVVPYNGGWVLHDPMPFGEHLVYDAADIELNIKTEAGVVIAAGAPS
                     EPNGEWTRYRLYGARTFSISASDEFLVTESAVGSVVIRSYYFDGYGAAADGILYGAVQ
                     AVSLFDVKFAPYPYQSLAVVQTDIHDGQEYDGLVFLSTDFYSQYGGGAKNNLTTIGVH
                     EIAHQWWFGLVGNDQALEPWVDEAMAVYSERIFYEYNYPRYGDWWWQFRVDYFGAGGY
                     VDASIYNFGDQRSYVNAVYLNGAHFFEDLRIRMGDDNFFRFLKDYAAIHSRGRASSFD
                     LFAAIRANTSADISDLIAAYFSQSY"
     gene            complement(13482..14972)
                     /locus_tag="PL000_06435"
     CDS             complement(13482..14972)
                     /locus_tag="PL000_06435"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNC88569.1"
                     /translation="MRKNIQSSISTLFVFIFLLSSCSTPSPSPTALPTSTAIPFSTST
                     PLPTASNTPLPTATTVPAPERARYVLDTLIDYDAHTVTVDETIYYPNHTGNQLNALVI
                     AIVPNLWEGSFNLNGLSINGTPITTYNINGQRLDISLPFFFKPEEVMVINIQFTLNLP
                     FAEQEDPSVSRPRIYGYTRRQLNLTNWYPFVVPYINGDWVLHEPWYYGEHLVYDAADY
                     QVDVRFADPANVPIVAASGAPEQQADSTRYTITAARTFALAASPEFQVSSLQVGDVIV
                     ASYYFPLSKIAGRAALQASAEALQIYSQKYGPYPHKTLSMVMGDFNDGMEYSAFFYLS
                     RDFYSLYDETPANYLIFVAVHETSHQWWFDQVANDQAQQPWLDESLATYSELVYYETL
                     HPDLVSWWWAYRIDFYNPQGFVDIPIYDGQGFRPYTNAAYFQGAHFLSELRARIGDDA
                     FFAFIQDYLLQGRNKIMTTSDFFRILGEHTLTDTSDIVRQYFQNIY"
     gene            complement(15069..15512)
                     /locus_tag="PL000_06440"
     CDS             complement(15069..15512)
                     /locus_tag="PL000_06440"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC88570.1"
                     /translation="MTIIEASIKINKSVEDVFEHLTNIDNMKKMSEYITAIESDGPLK
                     LGAKYKITTTSFGKSTTITNEIIAFEKNKLISTKTYGTPPAPDLVNTSILEADGSGTK
                     LTGQMDAVLLPPGMPNMPGMEDMMKKQLITGIENTLASYKKAIEG"
     gene            complement(15559..16374)
                     /locus_tag="PL000_06445"
     CDS             complement(15559..16374)
                     /locus_tag="PL000_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNC88571.1"
                     /translation="MNWLLEPLAYEFMQHGLLASVIVGVLCAVMGTYVVLRGMAFLGD
                     AMAHAILPGVAIAYIFKGDLLIGAGIAAVAVALIIGLFTKDGAVKEDTAIGILFAAAL
                     SLGVALISTTQTYTVDLSHILFGNVLGVSVSDLWLIGGLSFAILLTVALLFKPFLVIS
                     FDPVLAATLRLPVELLRNLMLVLLALTVVVSLQTAGVSLAAAMLVTPAATAYLLTRRL
                     LPMMFVSALIGALSSVVGLYLSYYFNIVSGSAIVLTATAIFLLGFLWKRIKSV"
     gene            complement(16371..17177)
                     /locus_tag="PL000_06450"
     CDS             complement(16371..17177)
                     /locus_tag="PL000_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNC88572.1"
                     /translation="MTHTPHRLEVQNISIGYGDKIIMHDLSFQVPHGSRVAVVGPNGA
                     GKSTLFKALVGLLPLKSGQIFIHGEALGVHKDCVAYVPQREEVDWRFPVTVNDVVMMG
                     RFGQTGWLNRASAYDRGMVHKSLEQMGIADLAEHSIGQLSGGQQQRAFLARAIAQEPH
                     ILLMDEPFTGVDATTQETTLRLLDHLQEREVTAIVSTHDLNLAASRFDLVLLLNHRLI
                     AFGKPQEVFVKENLAQAFGNSLLVMEGGMMLVDECCPPDEEHHHHHVEAE"
     gene            complement(17239..18177)
                     /locus_tag="PL000_06455"
     CDS             complement(17239..18177)
                     /locus_tag="PL000_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNC88573.1"
                     /translation="MKKIFNTIIVMLTLAALFLTSCGSASTGSDGTLSVLASTSFLAD
                     IAQNVAGDRTQVQSLLPIGADPHAYQPAPADVAKIAKSTVLIVNGVEYEHFIESLLEN
                     AGGERLVIEATAGLTPLQMEEHTDEAESGDSHGNEAGDPHMWLDPNLVITYVENIRDG
                     LSKADPAGAETYASNAEAYITQLKELDAFITEQVETIPADRRLLVTNHEAMGYFATRY
                     GFSIVDSILPSFSSEASASAQEIAAAVDAVKNSGAPAIFLGEVENADLANQIASETGV
                     KVVDGLYLESLTDGGPASTYIEMMKNNVIQIVNALK"
     gene            complement(18230..18646)
                     /locus_tag="PL000_06460"
     CDS             complement(18230..18646)
                     /locus_tag="PL000_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNC88574.1"
                     /translation="MSSENWLQQLHDNGYRVTSARRAVVDTVYGNSHALTPIEVYDQA
                     RKKYRALGLVTVYRTLEKLEELHLIQRVHQPIGCQAFISAGVGHQHLLLCQNCGQVEF
                     FEGDDLDTLTKSIARKTGYQIQEHWLQLFGLCANCK"
     gene            complement(18767..21283)
                     /locus_tag="PL000_06465"
     CDS             complement(18767..21283)
                     /locus_tag="PL000_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNC88575.1"
                     /translation="MKKTKLTLSLLLLLLVFTQAVPVLAHPADVYTHHIYVNIAQGGV
                     FIKWEIRPGPLLIQSIWFNADADQNGTVSDEEGLAWANDHLSKITSLLNGVKMPLTLD
                     GVKFPSSVDLFQAGQEFIVINLSASPIDSDAAYQLIFRNGFEEQKSLNWYYITTTDGM
                     KFETPQQKNAQIALKIFPPSTQDSAQVKLLDAWDSSMPSLSGWQGGTPPATPEPAAPT
                     PEQNANLERLLNLVRAKEFSLSFYIFALGISLILGALHALTPGHGKTVVAAYLVGSRG
                     TVWHAVVLGSVVTLTHTGSVFLLGIITLAASKYIVPTDIIPWLEILSGLLIVGLGLYL
                     LWQRFVYWWKSRQPAPVEEKAARKISLTPSAAPKKVTGVTGIQKPSANMHHHGDGKLH
                     SHDVPEAITWRSLIALGVSGGLVPCPDAIAILLVAVAINRIFLGLALIVSFSLGLAVV
                     LIAIGLLMVSSRRLFDKVSFLDKVAPIMPIVSAVVVLALGFALTWGAYVRAKDSLGLG
                     EAGTGGVEDARIVYQLEGQDRIKQLFISDIRGSQPEALTSGPNSAVDFAPSPDGSRIA
                     YIAQTGDLKNEIWLVDVNSGENKKLTDCTEAMCSQPVWSPDGSRVLYEHISLEAANAT
                     GLATLWWVDVNTGTARSLFQEEQLPGANPRWSPDGKWLSYATSENIQLYNLESGETRT
                     VASALGASAAWAPDGKSVLYRDVIIQSNQFITQLFIYDLETDAAVNISPDLGFENILA
                     AWSPDGAWIAVVRRDLSIPRGDQIWVMRRDGSEARAVTDSPNSLHGTLSWSGDGQSIL
                     YDLYDLDAFPLASKLQMVEAATGKVTDLGIAGYNPKWLWP"
     gene            complement(21291..22652)
                     /locus_tag="PL000_06470"
     CDS             complement(21291..22652)
                     /locus_tag="PL000_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88576.1"
                     /translation="MKPRITLDTVALSLIALLVVAAGLVILFSGQIGVRVTATLPERS
                     LIGPFQKIKLTFSESVDHELAESLFSIQPKVDGTIEWLDARTMQFVPTRPFERGASYK
                     LTLSPGRLTQNGSEIKSSQSWDMRVRDPLIVYLVANNDQSILWTVELDGSSPQPISPE
                     EIKVIAFDASADGESIVFSSANSQGGIDLWRMSRAGGDAAIMLDCGRDRCTTPAIAPN
                     GVLIAYTREAAGVGPGLPYGSPRIWVLDLQNGETGPVYEDQQVLGYKPSWSPDSSKLI
                     SFDGLADQFRLFNFTNNEQILFQSNTGGAVTWSPDGTKFLYTDIDLSENGLVTRLRLA
                     DLALNDTSTLLGNSDSRDYAYNSMAWSPSEDSVVVSLRNNEGEPAQVFWLFDPGLLDG
                     IIIADQPGYTYNSPLWDPWGTALVFQQFKLRGEFTPEIGLWQHGFREPLTIAEGIMPH
                     WLP"
     gene            complement(22649..23398)
                     /locus_tag="PL000_06475"
     CDS             complement(22649..23398)
                     /locus_tag="PL000_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HNC88577.1"
                     /translation="MPKTLYRSFQALLLLGLCIFFASKAASGQLTYYINLRFVPLTIF
                     GIILLAILAQTLFTEIKRSRQRKEGDHDHDHDHEHEPVSANLWFMLLPLLIGVLIPAR
                     PLDSSALATKGFNTNAPLVSADSSAQLFETDSEERTILDWIKLFNYQSDLEQFVGQKA
                     SVIGFVYFTEELGENQFFVSRFVVSCCAADGFAIAMVGEWPQASTLEKDAWVLVKGPI
                     KIITLNDRPVPLIQAESVQSVPVPEQPYLFP"
     gene            complement(23408..24394)
                     /locus_tag="PL000_06480"
     CDS             complement(23408..24394)
                     /locus_tag="PL000_06480"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNC88578.1"
                     /translation="MSRTSIVTIALVVLVLVVASMLPAGFFAVVWERLNVFATVFLGI
                     FVEAIPYLLLGTLASGLVEVFLDRDQMSRWISRRPVAAAVGGALMGMVFPVCECGVVP
                     LTRRLFNKGLPLSAGIAFLLAAPVLNPIVVLSTASAFGWGQMLLWRMGVSLLIAVVVG
                     LVFSVEQDASAVLRPVLTSAHEHDHSMDASASFNEKMRRALLVTADEFFEMGRYLIIG
                     AMLAAALQTFIPQSSLLAIGSGPVTSVLVMLALAILLSICSTVDAFVALGFFGTFSFG
                     SVLSFLVFGPMVDIKSVIMYLQVFRRRSVAYIVAIPFLMSLLAGILFNYLQP"
     gene            24578..25705
                     /locus_tag="PL000_06485"
     CDS             24578..25705
                     /locus_tag="PL000_06485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC88579.1"
                     /translation="MFKRLFGQILLHLGEMEEEFRLFHLRDDIATFIITAVCLLLANA
                     IMLRVDFILYRSSMDLFYLIIPLRSLQVFLSGAFLFLVYRTTQVQKFDMFVFTWLLLN
                     VIYFSLFNFTRPADYMATTFDLLFIFGLYLLSPLQLKQTLALSVSLSLGSLYVTYYYK
                     SGIPYFIVGSTTGAHLFIHMLGLASALQIQSFKRRAFQAYARERKAHEITQDLLRIDV
                     LTKALSRRYFFEQAQWEFERSKQLNLPLSVLIIDLDHFKKINDSNGHHAGDEVLSRFG
                     HFILHQKRSQDLFGRLGGEEFSIVLPEAKTKEAQNAARRFQEAWVDTPVEVDSKIIHS
                     TMSIGIAELSQSDRNFDELLNRADSMMYKAKQRGRNRVAHK"
     gene            complement(25716..25928)
                     /locus_tag="PL000_06490"
     CDS             complement(25716..25928)
                     /locus_tag="PL000_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88580.1"
                     /translation="MKKIDRRGKLQENPFTFKETADGKVFIYWHGRQVKTLKNGAAEK
                     FLQGLTDENAQLRMARVTGNFKRGNE"
     gene            complement(25940..27484)
                     /locus_tag="PL000_06495"
     CDS             complement(25940..27484)
                     /locus_tag="PL000_06495"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC88581.1"
                     /translation="MQLSPPSITYSSFLLIASITCLFVAVIVSRSRKNAAGANSLIAL
                     LLALAWWDATYGLFWAGTPGPTEYFWLDITYVGAVIVPGALFSFALQITNRQEWLKRP
                     FTLILYLEPLLVLTCLFTDPYHGLFFAGKRAENSAVILDAGPVFWANVAYSYTLILVA
                     TALIVGGFIRASGIYRKQLGLVAVGIGVTWLNSIVFIVGLNPLPGADNTPFSFTVAAV
                     AFAFAVSRYHLLDLIPVARDILIEKMRDGVLVIDSHNRIVDMNPAAQNLLNINSDKIG
                     KPVEEVIKKWQHYEKNTLPQIEMEIRRKTKRYVDMQTSFIVDDKGRNIGRLIILHDIT
                     KLKKVQSELHLLANSDSLTGAANRGYFMSLARKEIRRALRYKRKLSLVLMDMDFFKKV
                     NDTYGHASGDHALIALRNICINHIRKEDTFARLGGEEFALLLPETSQKTALHLAERLR
                     EALASTVINVDTLKFKVTISMGVTEFGIQEQDTFEGLFHRADKALYKAKADGRNRVVK
                     WHKGLG"
     gene            complement(27621..28037)
                     /locus_tag="PL000_06500"
     CDS             complement(27621..28037)
                     /locus_tag="PL000_06500"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNC88582.1"
                     /translation="MKSYRASISINASPEKIWKILSDASSYPAWDLSMDHIDGRLALG
                     ETVTFFTKLSAQSFPVKVTVFEPGKKMVLTGGLPLGLFKSERTHTLTPSKDGGTDFHT
                     EEIFSGLLLPLFGRNIPDLTSNFEGFVSALKRQAEK"
     gene            complement(28050..28409)
                     /locus_tag="PL000_06505"
     CDS             complement(28050..28409)
                     /locus_tag="PL000_06505"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC88583.1"
                     /translation="MKHAKSKNDIVNEYIGELDVEIAPLFLEIRACVLAAGPVLNESI
                     KWKNCLVYGTTKNHIQTVVGKGRVSLIFFEGASLHDKYGLLEGDGSKTRTMRIASMDF
                     NKAALKDYIKQIMKNEK"
     gene            complement(28432..29217)
                     /locus_tag="PL000_06510"
     CDS             complement(28432..29217)
                     /locus_tag="PL000_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88584.1"
                     /translation="MKISFQKKMGDPSLGILFFWKMTMESPMSVMSVEHFIPEPFFDY
                     LFIQKGQVHIVDGGAGRKSALPHQSLRNLYTRPVKLFFSTPLVLFGARFSLKFAESFS
                     GEIKANCFLKQDWVKRDTNDLEAFTLQVMEHVRMQQHPKLPHPILSLNFKESDWLDNF
                     SARHKRRLYQKVFGLSRKDLQNIQNVHVFLGQVCDFSARNPRIIEHVTPDVFYDQPHL
                     NRSFKKMTGLAPVEYFQNSSILQDNLMSASYNEVLPAVDKIGI"
     gene            complement(29214..29813)
                     /locus_tag="PL000_06515"
     CDS             complement(29214..29813)
                     /locus_tag="PL000_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88585.1"
                     /translation="MDLEAQKALQVELDAADVRWAASAAHASIEKWSQRDGYYNNRLA
                     SHLTGKLGELATEKYLLENRFKLDSHFRFAERENLCDIVVKVKNYSQVCRLEVKTWSA
                     SYWAELGRCIAVDQYPTLKKKADLVIWCVLAPVDMDQLTASPSPLVVSLAGWSTMAEI
                     SKAPIHATGSGGMRKVTNYQLEEKSLHPFVEFGDELEHR"
     gene            complement(29833..30834)
                     /locus_tag="PL000_06520"
     CDS             complement(29833..30834)
                     /locus_tag="PL000_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88586.1"
                     /translation="MTTTHQRKNLGYWLSRIGRILLILLIIPLPIGWVWQVIAQRVEG
                     RQNLPLGQLTNVDGHMMHIYCTGEGSPTVILEGGVPEWSIHWQKVQPEISKFTRVCSY
                     DRAGYGWSEAGPNPRTAEVIVKELHTLLRNSGETGPFVYVAHSFWGPAALLYQNNYPD
                     EVVGMVLIETWSPDLFIPVPDVITQSLPLAQTLKAIAPLGQVRLVGELGILPLSEMLQ
                     AGLLPEELQPAYKAAYYDDGMWSAVAGEYSAMEQSGIQTQGLVSLADLPLVVVKAGIR
                     PADDYPPDEIWDTTQDYLASFSSRGRLVVVENSGHFVQLEQPALIVDLIRQVVEETK"
     gene            31025..33013
                     /locus_tag="PL000_06525"
     CDS             31025..33013
                     /locus_tag="PL000_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88587.1"
                     /translation="MHGYGWFMAMRQGDEKPKVTRELLLRVLNYAKPYWWHITGMLVT
                     ILLSTGLSLLTPLIFRNMIDVVLPAKDTQRLIWLGLALLFIPALNGAISVVQRRLNST
                     VGEGVIYDLRASLFSRLQRMSLRFFTNTKTGELMSRLNSDVVGAQNAISNTIVNIVTN
                     LIEAIALFAVMISLEWRLTLVSILILPLFIITAQRLGTVLRDIARQAMDTNAQMNAHM
                     NETLNIGGALLVKLFGRTREEEKRFRERAANVRDIGIRRAVIGSTFFVIFGLVTAVGT
                     ALVYGLGGYFVITDVFTVGTIVAFGSYLGQLYGTLQGLASAPVDFSTSMVSFERVFEI
                     VDLPQDIAEKENAIELQDVQGELEFKDVTFNYKVDESILLKDVKRYGSMDNVKAVISG
                     QPSVDGKNSQGGNGSSSRIKNREERNAASDSPEPDSTSQARDIALENISFVARPGQLI
                     ALVGPSGAGKTTVTYLIPRLYDPTDGIIRIDGHNLKDVTLDSLAHAIGMVTQETYLFH
                     DTIRTNLTYAKPEATQAEIESAAKAANIHNFIMDLPEGYDTIVGERGYRLSGGEKQRI
                     ALARVILKNPRILVLDEATSSLDSESEALIQDALKRVMAGRTSIVIAHRLSTILAADL
                     ILVMDRGKIAERGTHEELLAAGGIYSQLYETQFRGERV"
     gene            complement(33043..33495)
                     /locus_tag="PL000_06530"
     CDS             complement(33043..33495)
                     /locus_tag="PL000_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC88588.1"
                     /translation="MHPFFSDYLNLLQKCHNDIHAVLNDLPAEALDWGPGQEMNSICV
                     LVYHLTGAERYWIGDVAVGESSNRDRAAEFKVRGLGMDFLKKRLDDSFDYAASTLERL
                     SLQDLDRLCVSSKGECTVAWALLHALEHSTLHLGHLQITRQLWEKLAG"
     gene            complement(33497..34315)
                     /locus_tag="PL000_06535"
     CDS             complement(33497..34315)
                     /locus_tag="PL000_06535"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNC88589.1"
                     /translation="MKHISLDHPLARGRTADVYKWDDGYVLKLFHHWFPLEDIEYELR
                     IARAVHASGIKSPVAHELIRVNGRNGLVYDRVNGESMAAMLQHKPWKLFACAKTLAQL
                     HARMHDFVFGADMPKLRGKLQRKIENAPALPTPLKTALLTVLHSLPESDCVCHGDFHF
                     DNVLLTESDPVIIDWMDTSRGNPLADVARTSIILLGIVENREMHNPALKFLVRVFHSI
                     YLREYFRLRPQGRAEYIRWLPIVAGARLSEQIPELEGWLTAKAEEIYQYIGMEQ"
     gene            34389..34961
                     /locus_tag="PL000_06540"
     CDS             34389..34961
                     /locus_tag="PL000_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC88590.1"
                     /translation="MVKIENSKKTILNLAEALLQDKGFNGFSYAHIASELGVKNAAIH
                     YHFPTKEDLGQAVIQRYRDRFKLWVNNSRVKDLAPQEKLDWFFSIYTAMRSDQGKVCL
                     VGSMEVEFNSIPEGLQAEVQAMHKELLAWLEGTLTEGRAAGVFRFNGEPANKAALILS
                     TLQGGLQMARALGTKKFRDVIEQLKLDLLA"
     gene            35014..35826
                     /locus_tag="PL000_06545"
     CDS             35014..35826
                     /locus_tag="PL000_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88591.1"
                     /translation="MTTLESTATKYQGIVKKAYQAFFSPSQYEVKSADREIIARGNNY
                     RISFEGGELAVTTWGESGPAVLLMHGWGGARAQMTGFVEPLLAAGYRVVAYDQPAHGE
                     SDGKMTNILEITPTMDLVEKREGKFDAIIAHSFGTLITSYALVHRNFPPPTRLVYLGA
                     FNRLLDSLPRFQVLANLPDEIIEGMRNMIYDNFGRGELDSIINESMVQQIHIPALMFH
                     DASDNVTPVDDSRAIASTWKSAQFIETSGLGHRGALQSKEVHAQIVDFLQKK"
     gene            35950..36282
                     /locus_tag="PL000_06550"
     CDS             35950..36282
                     /locus_tag="PL000_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HNC88592.1"
                     /translation="MKTALIFFFAVVSEVTATTALKFSEGFTKFVPSLIVVVGYGLSF
                     YLLSLSLKVIPIGTAYALWSGIGIVLTVIVGIVFWRETMDWARGIGIFLIMLGILVIN
                     LFSNSTAH"
     gene            36378..37349
                     /locus_tag="PL000_06555"
     CDS             36378..37349
                     /locus_tag="PL000_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HNC88593.1"
                     /translation="MNLRDLLRITSRTFAIGIERLPRVLCDATTIAYLLLRVSDYLED
                     NEEMEADKKVELLNLWVNILREEEAVETLTIQLTEANTENPDAIVAQHSKEILQHLRS
                     LPYEVQEIIVHHVINSTQGMARWTQHGSHLTDEKDLDDYMFEVAGRVGYLVTQLFAWY
                     SIAIRIKEKEIMPLAREFGLGLQTVNVIRGLREDFNRGWVYVPQKFLSEMGLTKEQLF
                     DPQHSEQALKVLDMMTDKAERHLRNALQYVKSLPWWLHGIRLGCIFPLMFAIRTLAVC
                     RHNTQVFESEAKMTRNEVKKIVMDSTLWGWSNLWLDRYYQELNVVGE"
     gene            37471..37929
                     /locus_tag="PL000_06560"
     CDS             37471..37929
                     /locus_tag="PL000_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC88594.1"
                     /translation="MRLSKRGEYGLRAMIALAEPAEKGGAPQMMQIKEISLREQISPK
                     FLEQILLTLKNAGLLHSKMGVGGGYYLARSASEITLGQIFRVLDGPVAPIKCVSQMAY
                     EPCGCPDEETCGLRLAMGDVRNAIADILDNTSLADVTKRQSTVRKKLGIK"
     gene            38032..>38447
                     /locus_tag="PL000_06565"
     CDS             38032..>38447
                     /locus_tag="PL000_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC88595.1"
                     /translation="MRKQVLTWLSVFLVLAVTLTACGTAASEATAVPQPETGTSSEEP
                     LSGTISISGAFALYPMMTVWAEEFTKLHPDVEFDVQGGGAGKGMTDTIAGAVDIGMIS
                     RSIKEEETAQGIFWVSVTKDAVFPIINTENPVAAQIL"
BASE COUNT         9805 a        10218 c         9506 g         8918 t
ORIGIN      
        1 cgcggttgat gatcttatcg tccacgtcgg tgtagttcat gacgtgtttg acggcgtagc
       61 cccgatattc gaggtagcgg cggatgatgt cgaagacaag cgccgacatg gcatggccga
      121 catgagcatc gttataaacc gtcactccgc acacgtacat tttgacgagg ttgggttcga
      181 gggtttggaa ttcttcggtt ttgcgggtga gagtgttata gattttcaac atggttctat
      241 tttaaccaca gatgaaccca gatgaaaggg attaataagg gcaaaataaa acagggcgcc
      301 tctcgacgcc ctgttaccga ccactttcac caactgcttg ttatttactt gcctttgccc
      361 tttttgccac tgttctcttc atccacgcgt ccaaagatca tacggcctgc ggcggtttgc
      421 agcaccttgg tgatgttcac ttccatgtac tcgccgatgt aatctttgcc gttctccacc
      481 acaaccatcg taccgtcatc catatagccc acaccctgac catattcctt gccttcctgc
      541 atgatgttga tgcgcagggt ttcgccgggc agcaccaccg atttgacggc attcgcaagt
      601 tcgttgatat tgagcaccgt cacaccctgc aattcggcaa tgcggttgag gttgtagtca
      661 ttggtcagca ccgggctctt gagttgtttg ccgagcacga tgagtttgtc gtccacttca
      721 cgcacgcctt ccacattgat atccgagatg cggaccagca cgttcgggag tttctgcaat
      781 tcagagagca cttccatgcc gcggcgtccg cgctgacggc gcatgccatc gggggagtct
      841 gcaatgtatt gaagctcgtt caacacaaaa cgcggaatga gaagcgtgcc gggcaggaat
      901 ccagttttgg caatatcggc cacacgacca tcaatgatga cactggtatc aagcaggatg
      961 ttgcggttga gattcgtcca cgaggaagaa ccgccgcctt caccacgtcc acttaatgca
     1021 ctgagcagtc ccataatgtc accttgacgc atggtgaaca gggaaatgcc gagataagaa
     1081 aagaccagca ccccaagaaa cggcaggatc tgaccaaacg gctccggcaa taacgaaagc
     1141 ggaaagccca acagcgccgc gatcagcagt cccacgatca gtccggtgag ggccgcaaac
     1201 aaactctctg ccgcaagcct gccaagcagt gagcgcaaat aacgggccgg gcgcgtggta
     1261 aagtatggag tgagaataat gccggccaaa gctcccacca ccgtggaaag cgtcgttgtt
     1321 acaattgggt ctgtttcagg gtttgccttc gtaaaattga acccccacat cccccctccg
     1381 atcgccagcg caaacattgc caaaatacga agaataaatt caaaactcat aataactcct
     1441 ttataatgaa tgcagaataa taaaggataa tagcacgggt accacccacc gtcaatcacc
     1501 cccgggaaga ttcatatatt attgttataa tctggaaaat accatggcaa tagaccgttt
     1561 tggacgaaat attcactacc tgcgcatcag cctgacggat cactgcaatt tacgctgtgt
     1621 atattgcatg ccggaggata tgaccttccg ccccaatgca gacctgatgc aggacgacga
     1681 gattctaacc ttcgcccgcc tgtttgccag cctcggcttc gataaaatcc gcctgaccgg
     1741 tggcgaaccg acggtccgag ccaacatcgt ggacattgtc cgcgggatct cctccaccga
     1801 gggcatccgc tccgtttcca tgaccacgaa cgggctcctg cttaaaaagc tggcccaacc
     1861 cctggcagac gccggactgc aacgcgtcaa catcagcatt gacacactca accccaataa
     1921 attcaaacgg ctcacccgct ggggcaaagt ggaggaagtc tgggaaggca tccttgccgc
     1981 agagcgggca ggcctcaccc cagtcaaact caacgcagtg gtggtcaagg gctacaacga
     2041 agaagatgtc attgacctgg cagcactcac cattgaccac ccctggcaga tgcgctttat
     2101 tgagatgatg cctttcggcg gtgcaaccga cctgcagatc aatcaggtca tcaccatgaa
     2161 acagatccaa gacaacatcg aatctgaatt tggaaagctt gaatccgcca acgacggcaa
     2221 actcgacggc gaagcgcagg tcttccacat ccgcggcgca aaaggaaacg tcggttttat
     2281 ttcctcggtc acgcacccat tttgttccgc atgcacccgt gcccgcctca cctctgacgg
     2341 aaaactgcgc ctgtgcctgc tgcgcgaaaa agaagtggat ctgctcacac ccatgcgagc
     2401 tggagcaagc cttgaagaat tacgcagact cctcctggat ggggtctggg aaaaaccctg
     2461 ggggcatggc ctggcagatg gagtcattcc actgaatcgg gtcatgagcg aaatcggagg
     2521 atagcattaa caacatcaaa gcaatcttgt tcgatcttga cggaacactt cgtcatcacc
     2581 tcccctctgg cggggaggtt tttgtggaat ttgtccgcag catgggcttc aaggtctcag
     2641 aagaagatcg catccgcgcc gaacactggg agcatttcta tttcgcctcg tcactggaaa
     2701 ttcgcaaaga tagcgaaacc tataaagatg atcgcagcgg tttctgggtc aattttggga
     2761 gaaggcgtct gatcgcattg ggcttgaaag ccagcgatgc ccttgaagtg gcaccgaaag
     2821 catctgctca catgaacgaa gcctacaaac ctcaggtgca tgtcccgccc gatgcgcata
     2881 cccaattgac ggcattgaaa gaagcgggct acattctcgg cgtggtatcc aaccgcgaaa
     2941 cgccctatca ggatgagttg aaggaattga aactcgattc ctatttcaaa ttctcgcttg
     3001 cgggcggcga ggtgaattcc tataaacccg acaagatcat cttcgaacgc gcattggaac
     3061 tggcgggaac ctccgcagct gagacgatgt acatcggcga caattatttt gccgatgtga
     3121 tgggagcgca tcgctccggg ctgaagcctg tcctctacga cccaaccaac ctcttccccg
     3181 atactggcgg cttcgcgttc atcaggtcat ttgctgaact acccgagtta ttgaaataat
     3241 caaaccaccc aacaccgcga atccacaaac gattcgcggt gttggcgtta aaatgaagat
     3301 ggaacacttg aaagacaaat gacaaaggag tccaacatgg cttgggaacg aaaactcatt
     3361 cgaaccgtgc gcgttcgcaa ttcacaaaaa acgggggtgc tcgcccgatt attgaccgcc
     3421 attgccgaag caggcggaaa cacgggcagt cttgttctat taactgaaac atcacagaac
     3481 gtggtgcgcg acattaccat cactgcagaa agcgaagcgc atttcgagac cattctggat
     3541 gccatgcgaa gcaatgaagg cacccgcatc atcgaaatac gcgatgaagt gctggaactc
     3601 catcaaaaag gcaagatcgc catccgttcg cgttacccca tcgaatcgct gaccaccctg
     3661 cggagagtct acacccccgg tgtggctgag gtctgccgca agatcgccaa agacccttca
     3721 caagcccgtc tgtacaccag catcagccac atggtggcca ttgttacaga tggaacggcc
     3781 gtgctcgggc tcggtgacat tggcccgctg gcagggatgc ccgtcatgga aggcaaagcc
     3841 atgctgatgg aaacgctcgt cggtctttcc ggaattccca ttttgctcaa caccaaagac
     3901 accgaagaga tcatctccac agtggctgcc atttcaccga catttgccgc catccaactg
     3961 gaggatattt ccgcaccacg ctgcttcgag atcgaagaac gattacaagc catgctcgat
     4021 atccccgtcc tgcacgatga ccagcatggt actgccgtcg tctgtacggc ggccctgctg
     4081 gtggccaccc gcgaaacagg cacagacttg aacaaggctg tcatcgggca gatcggcctt
     4141 ggagccgctg gtcatgccat cggtgtgatg atgatgcgtc tgacgggtaa tccagtgtac
     4201 ggtgcagatc taagtcccga agcgttggag cgctttgaaa aggcgggcgg gaaaaaatcc
     4261 aacttaagag aagtcatgga aaaatgtgac atcgtgatcg ccaccaccgg ggcgccgaat
     4321 ctcatcaaac ctgaaatggt gcgcaaggga cagatcatcc ttgccctctc aaaccccaac
     4381 ccggagatcg acccagaggt tgccatggaa cacggcgctg catttgccgc agacgggaaa
     4441 tcggtgaaca atgtgttggg cttccccggc atcttccgcg gcgcagtgga tgcaaacgcc
     4501 ccgcgcatca cacatgaaat gctgctggct gctgcacatg tcatcgcgga gatgacaccg
     4561 cccggcgaac tcgtccccag cccgcttgat aaaaaggtac atcgggccgt ggcacgcgcc
     4621 gtggcagaaa ccgccatcaa acaaggcatt gcccgtgccg aatacgtgcc ctatgtggaa
     4681 gaataaagat caaagataaa gacaaaagac ggcctgcctt tcgagcaagc cgtcttttat
     4741 ttttccactt tctatttttt acttcccgtt cttcaacgcc tctttcaagg tcaaggccag
     4801 gcgtgaaatt ccctcgcgga tgttctccgg gctggaaaat gagaaattga gacgcatggt
     4861 attttttcca ccgccattcg ggtgaaaggc cgcaccgggg acaaaagcca ctttacggtc
     4921 aatggccacc ttcaacactt cagccgcgtc cacattttcg ggcatcatcc cccacaggaa
     4981 catgccgccc tggggctttg tccaacgcac gtcagaaggg aacaattcat ccatcaactc
     5041 aagcatcaca tcgcggcgtt ccttgtaagt ggcgcggatc actttaacat gttcgtctaa
     5101 aaatccgcct ttggctactt cgtaggccac atgttgattg aacgatgacg tgtgcaggtc
     5161 tgcagcctgc ttcgtcatca ccagcctgcg gatcacctgc ggcggagcga tcacccatgc
     5221 caggcgcagg ccaggtgcaa gcagcttcga aaaggtgctc aggtaaatga cgttccccgt
     5281 gtactcgcca ttgtcttcat tgcgataacg gctgtcgagg gttgccacag atgggatatg
     5341 ttcgccatcg taacgcagct gaccataggg atcatcttcc acgatcggca caccgtactg
     5401 gtctgccaat aacaccagcc gtttgcggcg ctcaaggctc attgtagagc ccgatggatt
     5461 ttggaaattt ggaagcacgt aaataaattt cggcccgatt cgcagcgcct cttccaattg
     5521 gtccacgatc atgccgtctt catccgaagg caccgaaata tattgggcgc cgtaggcgtt
     5581 ccacgcctgc aacgccccca aatatgtagg cgactccacc actacataat cgccgcgatt
     5641 aatgaacaac ctgccgataa aatccagcgc ctgctgtgaa cccgaagtga tcatgatatt
     5701 gtcagaggtg acccgcacac tgtaacgcgc attgtgcctt gcgatcatct cacgcaatgg
     5761 tagatatccc tcggtggtgc tgtactgcaa tgcctgcggc ccatgatcgg tgagtacctg
     5821 attacaggcc tcctgaaatt ccttcaacgg gaagacctcg ggggcgggca atccgcctgc
     5881 aaacgaaata atatcgggct gctcggtaaa cttcaacagt tcgcggatga ccgaacttcc
     5941 cattttttgt gtacggtggg cgtagcgata ctcccagggt gtctgcattt tttctccttg
     6001 atgaacggaa taaaaaagcc tgacaggtac gtgtgtaccc gtcaggctgg ctgacttaag
     6061 gcattaatat gcccaaaagg tcgaaggccg ctattctttc catggcacct ttatcttaac
     6121 cattttcagg cgggactgca acctcatttt gtatgaaacg ccttatgaca aatttcctgc
     6181 aatgatatgc tcaaccgaag tccgcgtgta agaatcaagg ggcagatgaa gagcctcgct
     6241 cggactcacc caaacatgat cctccgcctc atcatttagc tgcaccgact ctgaatccgt
     6301 cctgcaggcg taatcgaaga agataaaatg ccgccgcttc caaaacgaag ggtcgtaaat
     6361 gaactgctga aaattgatga acttcaggtc gtaaatatcc agcccagttt cctccttcgc
     6421 ctcgcggatc gccgcctcct ccagccgctc acccaattcc acgtgcccgc ccgggacgac
     6481 atacttgccc gggaatttat ggctttgcag caaaagcagt tcccctttct tgttgaaaat
     6541 aaacacacct accgtcggtt ctggaaagat ctggtctgcc attattcacc tatgaaatct
     6601 tcgcaagggc gaatcacccg aacactgcaa tcaccatcac gcctgcaaaa acgatcgccg
     6661 accccaccac acgaatgccg cccatttcct ctttcaaaaa tctccagccg aggaaggcgc
     6721 cgatcacggc actcacttca cgaatcgccc cggaatagct cagcggggca aaggtgtacg
     6781 caaacaaagc cagcatgtaa gccaccaacc caagcagccc gcccatcaaa agatatcctc
     6841 gtttttgccg ccacatcgct gaaaaatgac tccatccata ccgctgaacc agatacggcg
     6901 ttgtcacgaa cggaaccatc acaaacatgg acaacccgta cggcagggct ggtccatgct
     6961 ttacggcatt gccgtcaatg taagtgtaga tcgagatgat caaagccacc gaaagcgctg
     7021 tgaggattcc acgcaagtgg ggcttttcct cgtggttttg caataaggtc gttgcgccga
     7081 tcaggatcat tccgcctgtg atcatcgcca ggccgatata cccgcccgtg gtcaattgct
     7141 catgcaaaaa gagcgccgtc cacaacacga gcagggcagg ggccgctccg cgtgcaatgg
     7201 gatacaccag cgaaaaatca tggtcgctat aggcgatgca gagcaaaatg aaatagatcg
     7261 cttcaaggat catgctgatg acagcaaagg tccacatcga acgcggcggc aggccggtga
     7321 agaagatcaa caccgtggag aagacaccgc tcaggatcac ctgccacccc atggcaatgt
     7381 atttttcctc cgcgcttttt aacaaaaaat tccacgtagc atgcatcgcc gcggaaaaga
     7441 acagcagcac aagggcaagg atcggcatga attacctcag aaactcgatc agatttttat
     7501 tcacttcatc ggcttcatcg tgctgaaccc aatgtgtgga cttttcaaaa agcacggttt
     7561 ttccatcatc gcacaatgca attgacggtg ccaccatttc gtggctcagc gccacatccc
     7621 gttttcccca cagcatcaaa gttgggatag tgattcgacg ggcggaaatc tttttaccaa
     7681 acacatgcca cagactacgg cgaaacacgg cacggtacca attcagcatc cccgtcagcg
     7741 caccgggctg tgcccatgct tttttatatt ccgccaggtc ttgtaatgtg aatgtgcttt
     7801 ttcgccccga ccccaccaac atccgaccga gattgtggaa gtcgtttttg ctcaacatcc
     7861 actccacaaa aagcggaatt tgaaagaaga acacatacca cgattttttg cgctgttccg
     7921 cattcttcaa cacaaattcc gtcatcacat cggggtgcgg cacattgagg atggcaagtt
     7981 ttttcaatcg ctcagggtga ttcagtgcca ctgtccatgc gacaacggca ccccagtcat
     8041 gcccgacgag gaaggccttc tcgatgccaa aatggtcgaa caagccaagg acatctctgg
     8101 caaggacatc cacagcatag gaggaaactc cctttggttt gtccgaaagg ttgtaaccgc
     8161 gctgatctgg cacgatgact cgaaatccgg ccgccgtcaa cgcggggatt tgttttctcc
     8221 aaccgtagct gaattcggga aagccatgca ataaaatgac cggcgtccca ttttcgggtc
     8281 cgtcggtcat tacatgcagg ttgattgaat tggtttttac aaattgactt tgcatcgcgc
     8341 tagtataacg gaattctggc tcccgttact ttattggctt cctcagagaa caggtactgc
     8401 atggcagaga cgatctcagc gggggaggtg cctttgcctt catttttcac gtcaattgac
     8461 tttacgtgaa tgacattcgc agtcaacccg ttttccttga attcctgcgc cagcgtcagc
     8521 accaaacttt cctgcgcgga ttttgcggcg gcgtacagtg ccatcttcgc agaaggattc
     8581 accgtcaccg gcatggatac ggcgatgacc cgtccccacg cctcgcggct gtttgcgatg
     8641 tgtggagcaa atgatttcag caggtgatag gtcgtccaca cgtgctggtt gatcatcgcg
     8701 tcaaggtcgg ctgcgtttga ttcagggatg gtcttgccgc ccgtccaccc gccgacgaga
     8761 tggatcaagg cgtggacact gccaaatttg gaacgaacag cttcggctga gtcctgaacc
     8821 gcctgcccat tgagcaggtc aatggtttgg gcgtggagtc tttcagaagg caggttcaat
     8881 tcacggacga gggattccag tttcgtttcg tctttatcaa gcaacgcaat gttgtgccca
     8941 tccgccgcaa atgttttcgc aacaaggttt cccaacacac ccgtcgcgcc tgtgatcaca
     9001 attgttttca tcttcaacct ttaatttcaa cgttcaaacg ttttaacgtt gaaacgtttg
     9061 aacgtttact catacagtgc ttgaatctga tcttcggtca tgccagccac aatcggcggg
     9121 tcgctctgtt ccacaagatg cggcttgccg tggaaatcga tcatgtgacc cgatttggcg
     9181 gctttggttt cgaggtagaa gcggttgtgt tcgttgctcg gcaaaatgtg cggcaggcgt
     9241 tcattgacct tcacaccatg ctgttcaagc tgcgcgatct tcttcgggtt gttggtcatc
     9301 aaacggatcg actggatctt gagcgagtgc agcatgtggg cggctacggc ataatcgcgt
     9361 tcgtcatcac ggaagcccaa tgcgagattc gcttccaccg tatcgaggcc ctgatcctgc
     9421 aaactataag cgcggatctt gttggtaagg ccaataccgc ggccttcctg gcgcaggtag
     9481 agcacaatgc ccttgtccat ttcgccgatc ttctgcagcg cggcttcgag ctgatcgcgg
     9541 caatcacagc gcagcgaccc aaccacatca ccggtcaaac attcggaatg caaacgcacg
     9601 ggcacatctt cagcgccaag cacttcgcct ttgatgatgg caacatgttc cttgccatcg
     9661 cggttatttg aaaacgccac aatgtgaaaa tcgccaaagc gtgaaggcag ttcggcaatc
     9721 gcttcgatat gaacgcaaat cttatccgag ccgtatcctt cacattcgtg acagcagtct
     9781 tcttccatga gaagctggat tttttcgtgt gtaaaagtag tcatggtttc ctccgtaacg
     9841 caagttgatt tctcacgcta caatctgtat ttcagaagga ccccgccgtc ctcccatttt
     9901 tcaacatcga tcaacttcaa acccattgcc acatcacgtg gcaggccgac cccatctgca
     9961 ggggtgggag cattcgcgcc gccaaagatc atcggcgcta caaaaatcat caactcatcc
    10021 acaagtccga ggcgcatcaa ctcaaaattc attgttccgc ccccctccac catcaaatgg
    10081 tccacgccga tctttttcag cgtcagcatc atcgaattca agtccacgcg cggggcatca
    10141 tgcacaaaga cctccacgcc acaggcggtc agttccgcga gtttgtcttt agatgtctga
    10201 tttgttgtaa atattacgac ccgtgaggga cctgctttaa tgaaatctga atcaggcggg
    10261 atatctgcat ttgcgatgcg tgtgaccacc cccaccttaa tcggattcgg gctcagccca
    10321 cgctgaaccc tgccctcacg cagcgcttcg ctcttaactg tgagcttggg tgcctcgtcg
    10381 agcagcgtcc gccccccgac gaggattcca tctgccgcgg cgcgcagctc atccacacgg
    10441 gttttatcgc gtttggatga gatggccgag ccttttcttt caaaggtgtc tatctttcca
    10501 tccgccgtca ttgcaacgtt gatgaatgta aatggacgat tcatggaata acaatgggtg
    10561 atagaccgtt atctttaccc aattcttacc gagcgcattg aaactgaacc accccaaacc
    10621 ttttctgcaa agaacgacac tttatcgtca tcatccttca atgccagcga atacagcagc
    10681 ggaacataat gctcaccgga gttgatcgcc aattgcgcgg cctgcccctg cttttggaaa
    10741 ttgatgatcg ggtcatggtc gttggcaaga atccagttct tcacctgtgt gtcgaattcc
    10801 gtggcccagt catacgcaaa atcacggaat tgcaccattg agaggttgtg tacgatgttt
    10861 ccagagctga tgatcagcac gccctcttcg cgcaattcct tcaactgctt cgccacctcg
    10921 taatgatcgt tgatcgaaag gttggcattc aaactcatct ggatcacagg cacattcgct
    10981 tcagggaaca tttggttcaa aacagaccat gtgccatggt ccaaacccca ttcctgatcg
    11041 gcgtagactt cggtcttctt gatgatgcgc cgcacctgtt ccgccaaatc gggcgaaccc
    11101 ggcgcggggt atttcacttc gtacaattcc ttcggaaaac catacatgtc gtagatcatg
    11161 cgcggctgtt ccatggcggt gaccagcgta ccgcgtgtaa cccagtgcgc ggaaacacac
    11221 aaaatcgcgc gaggcttggg cagcgccttg cctgcatcac gccaggcgcg cgaaaattca
    11281 ttatcttcaa tggcattcat cgggcttcca tgcccaacga acaggactgg cattttttcg
    11341 gtcatatgac ctccttagtg attggctttt ctgcgaacat ctttcaaaat atcggcaacc
    11401 gtgtaattgc ccagcgcctt ttccatttca gattgggcat ctgcaaatac agattgcaac
    11461 accccgccaa tgttcccgcc gatggggcag tgtctattcg ggtggctgtg gaccgccaaa
    11521 acattcacct catccaaact gcgatagacc tcgcacaagc cgatctcttt cggctcgcgc
    11581 aacaaccgcc agccgccttt tgcgcctgat ttggattcca ccaatccatg ctcgcgcaaa
    11641 ctcgccatcg tacggcgaat gaccacgggg ttggtatcca cactgctggc gatcatctct
    11701 gaagtcagcg gatcattgct tgaggcaagc aaggtcaaag catgaatgga aatggcaaaa
    11761 cgttggttgg tgttcataat tgtaaccatt atagttacga atacaaaaga gtcaatggtc
    11821 aggacagaat tcttaggaaa gattaatcac ctgacacaag gactccccca tactcaacgg
    11881 ttaatacgat tgggaaaagt aagccgcgat caaatcgctg atatctgcac tggtgttggc
    11941 acggatcgct gcaaacaaat cgaacgaaga tgcacgtccg cgggagtgaa ttgcggcgta
    12001 atccttcaaa aagcggaaga aattatcgtc acccatgcga atacgcaggt cttcaaagaa
    12061 gtgtgcgcca ttcaaataca cagcattgac atacgaacgc tggtcaccga aattataaat
    12121 acttgcatcc acataaccac cggcgccgaa ataatccacg cggaattgcc accaccaatc
    12181 gccatagcgc ggatagttgt attcataaaa gatacgctca ctgtaaaccg ccatggcttc
    12241 atccacccac ggttcgagtg cctgatcgtt gcccacaagc ccaaaccacc actgatgcgc
    12301 gatctcatgc acaccgatgg tcgtcagatt gtttttggcg ccgccgccat attggctgta
    12361 aaaatctgtg gaaagaaaaa ccaacccgtc atattcctgc ccatcgtgaa tgtcagtctg
    12421 caccactgca aggctttgat acggataggg cgcaaacttc acatcaaaga gactgaccgc
    12481 ctgcactgcg ccgtacagga ttccatctgc cgctgcgcca tagccatcaa aataatacga
    12541 gcggatcacc accgacccga cagctgattc agtcacgagg aattcatcac ttgctgaaat
    12601 ggagaatgtg cgcgcaccgt aaaggcggta acgggtccat tcaccgttcg gttctgaagg
    12661 agcacctgcc gcgatgacca caccggcttc ggttttgata ttcagttcga tgtcagcggc
    12721 atcataaaca agatgttcac caaaaggcat ggggtcgtgc agcacccagc ctccgttata
    12781 cggcacaaca aagggatacc aatctacaag attgatctgg ttgaaatcat agccgaacaa
    12841 acccgccccc ttagcaggga cattgagact gaaactgagc gtcagcgtgg tggctgagcc
    12901 tgccgggagt ggctggggca ggttcatggt cagagcctga ccgttgagtg tgtaagtggt
    12961 caacgcaaca gagtcctgcg aaatcgtatt caatacaaat gcgcccccgt ataaattcgg
    13021 ttgaaccgac aaaacgatat ccgaaagggt caaacctgtg ccattgtagt agcggatcgt
    13081 ttcttccaca tccagtgtgc gtgaggcaaa gtccattgtg gcatataaaa tgtaattcgt
    13141 acgcgaaggc gctgaaggcg gcggggatgt gaaagtgact gccgggggag ggttggtggg
    13201 cggcacctgc gtttgggtgg ggaattgggt aaacgtcgcc gaaggaggga cggtgggctg
    13261 ctgcgtcggt gtggatgttg gcaacaaagg gacgctcgta tcggtctgcg ccacgggttg
    13321 aaagggtgtg atggtgggcg atgtcgcggc gcgtgttgcc aaaatgaatg gcgcaaagac
    13381 ctgcggcgtg ggggcaggtg taggcgtggc acaggatgcg agaaaagaaa acagcaatac
    13441 tacagacgca atgcgtgtga gacgggaacg aatggggttc attaatagat attctggaaa
    13501 tattggcgca cgatatcaga agtgtcggtg agggtgtgtt cgccaagaat gcggaagaaa
    13561 tcgctggtgg tcatgatctt gttgcgcccc tgcaagagat aatcctgaat gaaggcgaag
    13621 aaggcatcat ccccgatccg ggcgcggagt tcgctgagga aatgcgcacc ttgaaaataa
    13681 gccgcgttgg tgtatggacg gaatccctgg ccatcataga tgggaatatc cacaaagccc
    13741 tgcgggttat agaaatcaat gcggtaagcc caccaccaac tgacaaggtc cgggtgaagg
    13801 gtttcgtagt agacgagttc cgaataagtg gcgagtgatt catcgagcca gggttgttgg
    13861 gcctggtcat tggccacctg atcgaaccac cattggtgcg atgtctcatg taccgcgacg
    13921 aagatcagat aattggccgg ggtttcatcg tacaggctgt aaaaatcacg ggaaagatag
    13981 aagaaggcgg agtactccat gccatcgttg aaatcgccca ttaccatcga aagcgttttg
    14041 tgcggatacg gcccgtattt ttggctgtag atctgcaagg cttcggcact ggcttgtaaa
    14101 gcggcacgcc cggcgatctt gctcagcgga aagtaataac tggccacgat cacatcccca
    14161 acctgcaggc ttgaaacttg aaactccggg ctggcggcaa gggcaaaggt ccgcgcggcg
    14221 gtgatggtat aacgggtgga gtctgcctgt tgttcaggtg ccccgctggc ggctacgatg
    14281 ggaacgttgg ctgggtccgc aaagcgcaca tccacttgat aatccgcagc atcataaaca
    14341 agatgttcgc cgtaatacca gggttcgtgc aatacccaat caccattgat gtaaggcaca
    14401 acaaacggat accagttcgt caggttcaat tgccgccgcg tgtacccata aatgcgcgga
    14461 cgtgaaacgc tggggtcttc ctgctcggca aaaggcaggt tgagagtaaa ttgaatattg
    14521 atgaccatca cctcttcagg cttgaaaaaa aacgggagcg aaatatcaag ccgctgtcca
    14581 ttgatattgt aggtggtgat gggcgtgcca ttgatggaga gaccgttcag gttgaaacta
    14641 ccctcccata aatttggcac aatggcaatg accaatgcat tgagctggtt ccccgtatga
    14701 ttggggtagt agatggtttc atccactgtg acggtgtggg catcatagtc aatgagcgta
    14761 tcaaggacat atcgcgcgcg ttccggcgca gggacagtgg ttgccgtggg aagcggcgtg
    14821 ttcgatgcag tgggtaaagg ggtgctggtg gaaaaaggaa tggctgtgga ggtgggcaat
    14881 gcagtgggcg acggagaagg tgtcgaacag gatgaaagca ggaagatgaa aacaaaaagg
    14941 gtggaaatgg atgattgaat attttttcgc atgggcagta ttctaccgct tttgagctgt
    15001 gcattgaaat aaaaactccc gccgtggatg cggcgggagc agatgtctac cagtaaagta
    15061 atgaggaatc agccttcgat cgctttcttg tatgaagcaa gcgtattttc aataccggta
    15121 ataagctgct tcttcatcat atcttccatg ccgggcatgt tgggcattcc aggcgggagc
    15181 agtacggcat ccatctgacc ggtcaacttg gtaccggagc catccgcttc gaggatggag
    15241 gtgttgacca agtccggtgc cgggggtgtg ccataggttt ttgtggaaat cagtttattt
    15301 ttctcaaatg cgatgatttc attcgtgatg gtggttgatt ttccaaaact ggtggtggtg
    15361 atcttgtatt tggctcccag tttaagcggc ccgtctgact cgatggcggt gatgtattca
    15421 ctcatctttt tcatattatc aatgttggtg agatgctcaa acacatcttc gacggatttg
    15481 ttgatcttga tggatgcttc aataatagtc attgtcaata ctccttgaat tggtctggtg
    15541 gataacaatg aaattatttt atacagactt gatccttttc cacaagaacc caagtaggaa
    15601 tatggcggta gccgttagta ctattgcaga gcctgacacg atattgaagt aataactcaa
    15661 atataaaccc accacagaag acagggcacc aatcaatgca gagacgaaca tcatcggcaa
    15721 caatcgacgg gtgagcagat atgccgttgc agcgggtgtg accagcatcg cagcagccag
    15781 actgacgccc gctgtttgaa gcgagaccac caccgtcaac gccagcagga cgagcataag
    15841 gttgcgaagc aattccacgg gcaggcgcaa ggtggcagcc agaacggggt caaaggagat
    15901 gactaaaaaa ggtttgaata gcagggccac cgtaagcaaa atggcaaaac tcaaaccgcc
    15961 gatcaaccaa aggtctgaaa cactgacccc aagcacattg ccgaacaaaa tatgtgaaag
    16021 gtccactgta taggtctgcg tggtgctgat gagtgccacg cccagtgaaa gcgccgcagc
    16081 aaaaaggatg ccaatggcag tatcttcctt gactgctccg tctttggtga acaacccaat
    16141 gatcaaagcc accgcaacag cggcaatccc cgcaccgatc agcaagtcac ctttaaaaat
    16201 gtaggcgatc gccacacccg gcagaattgc gtgtgccatt gcatctccca gaaaagccat
    16261 gccgcgcaaa acaacatagg ttcccatcac agcacacaac acaccgacaa taacggaggc
    16321 cagcaatccg tgctgcatga attcataggc aagaggctca agtaaccagt tcattccgcc
    16381 tcaacatgat ggtgatgatg ctcttcatct ggcgggcaac actcatcaac aagcatcatg
    16441 ccgccttcca tcacaagcag ggaatttcca aaggcctgcg caaggttttc cttcacaaat
    16501 acttcctggg gttttccaaa tgcaataaga cgatgattca gcaacaggac caggtcgaaa
    16561 cgtgaagcgg ccagattcag gtcatgggtc gaaacgatgg cggtgacttc ccgctcttga
    16621 aggtgatcga gcaggcgcaa ggtggtttcc tgggtggtcg catccacccc ggtgaaaggt
    16681 tcatccatca acaggatgtg cggctcctgg gctatggcac gcgccaaaaa tgcgcgttgc
    16741 tgctgcccgc ccgaaagctg accaatcgaa tgctctgcaa gatcggcaat gcccatttgt
    16801 tcaaggcttt tatgaaccat gccgcggtca taggctgaag cacggttcaa ccacccggtc
    16861 tgtccaaaac gccccatcat caccacatca ttgaccgtga cagggaagcg ccagtccact
    16921 tcttcacgct gggggacata tgcaacacaa tccttatgga caccaagcgc ttcaccgtga
    16981 atgaagattt gtccgctttt caggggcagc aaacccacca atgctttaaa caaggtggat
    17041 ttacccgcac cattcggccc cacaacggca acacgtgatc cgtgtggaac ttgaaagcta
    17101 aggtcatgca tgatgatctt gtcaccatag ccgatactga tgttttgaac ttcgagacga
    17161 tgaggtgtat gtgtcattgg attttcgatt cacgatttac ggatttcgag tgaaagacgc
    17221 gtttatttct ctcccatctt acttcaaagc gttcacaatt tgaatgacat tattcttcat
    17281 catttcaatg taggttgagg cagggccgcc atccgtgagc gactcaaggt acaaaccatc
    17341 cacaaccttc acacctgtct ccgaagcgat ctgattcgca aggtcggcat tttccacttc
    17401 accgaggaag atggcaggcg cacccgaatt cttcacagca tccaccgccg ccgcgatctc
    17461 ctgtgccgat gcactggctt cggaactaaa actcggcaaa atcgaatcta cgattgaaaa
    17521 cccatatcgg gtcgcgaaat aacccatcgc ttcatgattg gtcaccagca aacgccggtc
    17581 ggcagggatc gtctccacct gttccgtgat aaaagcatcc aactctttga gttgcgtaat
    17641 gtatgcctct gcattggagg cataggtctc tgcaccagcg ggatccgctt tgctcaaccc
    17701 gtcgcggata ttctcgacat aggtgatgac gaggttggga tcgagccaca tatgcggatc
    17761 tcccgcttca ttgccgtgac tgtcgcctga ttctgcttca tcagtatgtt cttccatttg
    17821 aagtggggtt aggcccgcag ttgcctcgat caccagccgt tcgccgcctg cattttcaag
    17881 caacgactcg atgaaatgtt catactccac gccattgacg atcaacacgg tgctttttgc
    17941 gatcttcgcc acatcggctg gagcaggctg ataagcatgc gggtctgcac cgatggggag
    18001 cagggattgc acctgggtgc ggtctcccgc cacgttctgg gcaatatctg cgaggaagga
    18061 ggtagaggcg agcacactca aagtgccgtc actgccagtg gaggccgacc cgcacgaggt
    18121 taagaaaaga gccgcaaggg ttaacatcac tataatggta ttgaaaatct ttttcattta
    18181 gagctcctat tttttatccc ccgccaatga cgagggatgg attaacgttt tacttgcagt
    18241 ttgcgcacag gccgaacaat tgcagccagt gttcctggat ctggtagcct gtttttctgg
    18301 cgatggattt cgtcagggta tcgaggtcgt caccttcaaa aaactccacc tgcccacagt
    18361 tctgacataa aagcagatgc tgatgcccca cacccgctga aatgaaagcc tggcaaccaa
    18421 tgggctgatg cacgcgttgg atgagatgaa gttcctcaag cttttcgagc gtacgataca
    18481 cagtgaccag acccaaagca cggtatttct tgcgcgcttg atcgtacacc tcgatgggcg
    18541 tcagggcatg ggaattgcca tacaccgtat ccaccaccgc gcgccttgcc gatgtaacgc
    18601 gatacccgtt gtcgtgcagt tgttgcagcc agttttcaga ggacatgttc accgtatttc
    18661 ttactgaaaa tcgttttcaa taagtatagt agcaaaaaat acgggtgtca acccattgtg
    18721 aaatgaaaat cgaaactctg cagtgaataa aattcgccga gcctgcttaa ggccagagcc
    18781 atttggggtt ataccccgca atccccagat ccgtcacttt gccggtagcc gcctccacca
    18841 tctgcaattt tgatgccagc ggaaaggcgt cgagatcgta caagtcatac aaaatggact
    18901 gcccatcgcc agaccagctc aatgtgccgt gcagagagtt cggcgagtct gtcactgcgc
    18961 gtgcttcgct gccatctcgt ctcatcaccc agatttggtc acctcgtgga atggaaaggt
    19021 cgcgccgtac cacagcgatc cacgctccat cgggcgacca tgccgcaagg atattctcga
    19081 aaccaaggtc gggactgatg ttgactgcgg catcggtctc cagatcataa ataaataact
    19141 gtgtgatgaa ctgattcgat tgaatgatga catcacgata taaaacagat tttccatccg
    19201 gtgcccaggc tgcagatgcg cccagtgcgc tggcaacagt gcgggtttct ccgctttcaa
    19261 ggttatacag ttgaatattt tcagaggtgg cgtaactcaa ccactttcca tccggtgacc
    19321 agcgcggatt cgctcccggc aactgctctt cctgaaaaag tgatcgagcc gtgccggtat
    19381 ttacatccac ccaccacagg gttgccaggc cggtggcatt tgcggcctcg aggctgatat
    19441 gctcataaag cacacgcgaa ccatccggcg accagaccgg ctgtgagcac atcgcttcgg
    19501 tgcaatcggt cagtttttta ttttcaccgc tattcacatc caccagccag atttcatttt
    19561 tcaggtcgcc tgtctgtgca atatatgcga tacgcgaacc atcaggcgaa ggagcaaaat
    19621 ccaccgcgct gttggggccg ctggtcaatg cctctggttg agacccgcgg atatctgaaa
    19681 tgaacaactg tttgatgcgg tcctgtcctt caagttgata gacgatccgc gcatcttcga
    19741 ctccgcccgt gcctgcttca cccaaaccaa gagaatcctt cgcccgtaca tatgcgcccc
    19801 aagtgagcgc aaaacccagc gccaacacca ccaccgcaga aacgatcggc atgatcggtg
    19861 caaccttatc gagaaagctg accttatcga acagtctgcg gctcgatacc atcaacaagc
    19921 cgatggcgat caacaccacg gccagaccca gactgaacga aacgatcaac gccaatccga
    19981 ggaaaatacg attgatggcc actgccacca acagaatcgc gatcgcatcc gggcagggga
    20041 caagtccgcc gctgacgccg agggcaatca gcgagcgcca ggtgatggct tcgggcacat
    20101 catgcgagtg cagtttgcca tcgccatggt gatgcatatt tgcagaaggt ttttgaatgc
    20161 cggtcacccc ggtcactttc tttggcgcgg cagacggggt gagcgaaatt tttctggcag
    20221 ccttttcctc caccggggca ggttgtcttg atttccacca gtacacaaat ctctgccaga
    20281 gcaggtacag accaagccca acgatgagca ggccggaaag aatctccagc cacggaatga
    20341 tatccgtcgg cacgatgtat tttgaagcag cgagagtgat gatgccgagc aaaaaaacgg
    20401 agccggtatg cgtcagcgtg acaactgacc cgaggaccac cgcatgccag acggtcccgc
    20461 gtgaaccgac gaggtaggcg gcgaccacag tcttgccgtg gccgggtgtc aatgcatgga
    20521 gcgctcccaa aatcagggaa attcccagcg caaaaatata aaacgaaagc gaaaactcct
    20581 tcgcgcggac aaggtttaga agccgctcaa ggttggcgtt ctgttcgggg gtcggtgcgg
    20641 caggctcggg cgtggcagga ggcgttccgc cctgccatcc tgaaagtgaa ggcatactgc
    20701 tatcccatgc gtcgagcaat ttcacctggg ctgagtcttg tgtggaaggc ggaaaaatct
    20761 tcaacgcaat ctgggcattt ttttgctggg gcgtttcgaa cttcatcccg tctgtggtgg
    20821 tgatgtaata ccagttaagc gatttctgtt cctcaaagcc gtttcggaag atcaattgat
    20881 atgcggcatc cgagtcgatg gggctggcag aaaggttgat aacgatgaat tcctgccctg
    20941 cttgaaacag gtctacgctg gagggaaatt tcacaccgtc gagagtcaac ggcatcttca
    21001 ccccattcaa cagcgatgtg atcttcgaaa gatggtcatt ggcccatgca agaccttctt
    21061 catcgctgac cgttccattc tgatcggcat ctgcattaaa ccaaatggat tggatcaaca
    21121 aaggccccgg gcggatctcc cattttatga aaacgccccc ctgggcgatg ttgacataaa
    21181 tatgatgagt gtacacatca gcaggatgtg caagaacggg gaccgcctgc gtaaatacga
    21241 gtaaaagcaa caacagggaa agggtgagtt ttgttttttt cattttcatt tcagggcagc
    21301 caatgcggca tgattccttc agcgatggtt agcggttcgc gaaaaccgtg ctgccataat
    21361 ccgatctcgg gtgtaaattc accacgtaat ttaaattgtt gaaaaaccag cgccgtaccc
    21421 cacgggtccc agagcggcga gttgtaggtg taccccggtt ggtcggcaat gatgatgcca
    21481 tcgagcaggc cggggtcaaa cagccaaaac acctgcgcag gttcgccttc gttgttgcgc
    21541 aaactgacca ccaccgaatc ttcagaaggt gaccacgcca tggagttgta ggcgtaatca
    21601 cgcgagtcgc tgtttcccag cagtgtggaa gtatcgttca aagccaggtc ggccaggcgc
    21661 aaacgggtga ccaatccatt ttcagaaaga tcaatgtcag tgtagaggaa ttttgtgcca
    21721 tccggcgacc atgtgaccgc gccgccggta ttggattgaa aaaggatctg ctcgttgttc
    21781 gtaaaattga acaggcggaa ttgatcggca aggccatcaa aggagatcag tttggatgaa
    21841 tcgggagacc acgacggctt ataccctaaa acctgctggt cttcgtacac cgggcctgtt
    21901 tctccatttt gcagatccaa cacccagata cgcggagacc catagggcaa ccccgggccc
    21961 acaccggctg cttcacgtgt ataggcaatg agcacgccat tcggcgcaat cgctggtgtg
    22021 gtgcagcggt cacgtccgca atcgagcatg atggcggcat cgccacctgc acggctcatc
    22081 cgccacaggt caatgcctcc ctgcgaattc gccgatgaaa agacaatgct ctcgccatca
    22141 gctgaagcat cgaacgcaat gaccttgatc tcttcgggtg aaatgggctg gggcgaactc
    22201 ccatccagtt ccaccgtcca caagatgctt tggtcgttgt tggcaaccag atacacgatc
    22261 agtgggtcac gcacacgcat gtcccaactt tggctgctct ttatttcgct tccattttgg
    22321 gtgagtctgc ccgggctgag agtcagcttg taactggcac cccgttcaaa cggtcgggtg
    22381 ggaacgaact gcatggtacg cgcatcaagc cattcgatgg tcccatccac tttgggttga
    22441 atcgaaaaaa gggactcagc cagctcatga tcgacggatt cggaaaaggt caatttgatc
    22501 ttttggaacg gcccgatcag actccgctcg ggcagggttg ctgtcacccg aacaccgatc
    22561 tgccccgaaa aaagaatcac cagaccagcc gccacaacca ataaagcgat caggctaagc
    22621 gcaactgtgt caagcgttat acgtggtttc atggaaagag gtagggttgt tcaggaacag
    22681 gaaccgactg caccgactcg gcctggatca gcggcacagg gcgatcattc agggtgatga
    22741 tcttgatcgg tcccttgaca agtacccacg catctttttc cagtgtggag gcctgcggcc
    22801 attcgccgac catggcaatg gcaaatccat cggctgcaca gcatgaaacg acaaaacggc
    22861 tgacaaaaaa ttgattctcg cccagttctt ccgtgaaata cacaaagccg atcaccgatg
    22921 ctttttgccc tacaaattgc tcaaggtcag attgataatt gaagagtttg atccaatcga
    22981 ggatggtgcg ttcctctgag tctgtttcaa acaactgtgc cgaagagtcg gcgctgacca
    23041 acggcgcatt ggtgttaaac cctttggtgg caagcgcaga agaatcgagc gggcgcgcag
    23101 gaattagcac accgatcaac agcggcagga gcatgaacca caggttcgcc gagacaggtt
    23161 catgttcatg gtcatgatcg tgatcatgat cgccctcttt acgctgacga gagcgtttga
    23221 tctcggtgaa cagggtctgt gccagaatgg caagcaggat gattccgaag atcgtcagcg
    23281 ggacaaaacg tagattaatg taataggtta attgtccgct ggcggctttc gatgcaaaaa
    23341 aaatgcacag acccaacaaa agcagggctt gaaaagagcg gtaaagtgtt ttaggcatgg
    23401 ggaattccta tggctgtaag taattaaaga ggatgcccgc caacaggctc attaaaaatg
    23461 ggatggcaac gatgtaagcc accgagcggc ggcggaagac ctgcaaatac atgatgacgc
    23521 ttttaatatc caccatcggg ccaaagacca ggaacgaaag caccgaaccg aaactaaatg
    23581 tgccaaaaaa gccaagagcc acgaaggcat ccactgtaga gcaaatggaa agcagaatgg
    23641 cgagcgccag catgaccagc acagaggtga caggtccgct gccaatggca agcagactgg
    23701 attgcggaat aaaggtttgc aaagccgctg caagcatggc accgatgatg aggtaacgtc
    23761 ccatttcaaa gaactcgtcg gcagtgacca gcagggcgcg gcgcatcttt tcattgaaag
    23821 aggcggatgc atccatggaa tggtcatgtt cgtgcgccga ggtcaacaca gggcgcaata
    23881 cagcggatgc atcctgttcc accgaaaaga ccagccccac cacgacagcg atgagcaaac
    23941 tgactcccat acgccagagc agcatttgtc cccacccaaa ggctgaagcc gtgctaagca
    24001 ccacaatggg attcaacaca ggcgcagcca acaggaaggc aatcccagcg gagagcggca
    24061 gacccttatt gaagagtcgc cgggtgaggg gcacaactcc gcactcgcaa acggggaaga
    24121 ccatgcccat gagggctcca cctaccgcgg cggcaacagg gcggcgcgag atccacctgc
    24181 tcatttggtc gcggtcaaga aagacttcca ccaaccctga agcaagggtt cccaacagca
    24241 aatagggtat ggcttcaaca aaaataccaa ggaataccgt ggcaaagaca ttaagccttt
    24301 cccataccac agcaaagaat cctgcaggca gcatgctggc aacaaccagc acaagaacta
    24361 caagcgcgat cgtaacaatg gatgttctgg acaataatcg tggggatgtt ttattttgca
    24421 caacaggatt atacccactg gccgccggtt tgttttgaat cgcaaaagat ttttttcacc
    24481 gcataatttc gataattctg ctcgtaaatt cacacttttg aaagcctgat ttacagcacg
    24541 tctttcaatt ccaccataag gtatgattgc gaaaaccatg ttcaaaagat tgtttgggca
    24601 gatcctcctg caccttggag aaatggaaga ggagttccgt ctcttccatt tacgtgatga
    24661 catcgccacg ttcatcatca cggctgtttg cctcctgctg gccaatgcga tcatgctgcg
    24721 tgtggatttc atcctatacc gctccagcat ggacctgttt tatctgatca tccccctccg
    24781 ctcacttcaa gtttttcttt caggcgcctt tttattcctg gtgtatcgca ccacccaggt
    24841 gcagaagttc gatatgttcg tcttcacatg gctgcttctc aatgtgatct atttctccct
    24901 gttcaacttt acacgcccgg cagattacat ggcgaccacc tttgatctgt tattcatctt
    24961 cgggttatat ctgctatccc cactgcaatt aaaacaaacc cttgccttga gtgtcagtct
    25021 atcccttggg tctttatatg taacctatta ttacaaatcc ggcataccgt attttattgt
    25081 ggggtcaacc acgggggcac atctcttcat ccacatgctg gggctggcct ctgcattgca
    25141 gatccaatct tttaagcgca gggcttttca ggcgtatgca cgcgaaagaa aagcccacga
    25201 gatcacacaa gaccttttgc ggattgatgt ccttaccaag gctttatcac gcaggtattt
    25261 tttcgagcag gcccagtggg aattcgagcg ttcgaaacaa ctcaacctgc ccctctcggt
    25321 attaatcata gacctggacc atttcaagaa gatcaatgac tcgaacggcc accatgcagg
    25381 ggatgaggta ttgagccgtt ttgggcattt catcctccac caaaagcggt ctcaagacct
    25441 gtttggcagg ttgggcggcg aggaattttc cattgtcctg cctgaagcca aaaccaaaga
    25501 ggcccaaaac gcggccaggc gttttcaaga agcatgggtg gatacaccgg tcgaagtaga
    25561 ctccaaaatt attcattcga ccatgagcat tggcattgcg gaactttccc aaagtgaccg
    25621 taacttcgac gaactactca accgtgctga ctcgatgatg tacaaggcca agcaacgtgg
    25681 acgaaatcgt gtagctcaca aataacatct tcttttcatt cattcccccg cttgaagttc
    25741 cctgtcacac gggccatgcg caattgggca ttttcatctg tcaggccttg caaaaatttc
    25801 tcagcagccc catttttgag ggtcttgacc tgcctgccat gccagtagat gaaaaccttc
    25861 ccatctgcag tttctttgaa agtaaacgga ttttcctgta attttccacg cctgtctatt
    25921 ttcttcattg agacttttgt taccccaatc ctttatgcca tttgacaacg cgattacggc
    25981 catctgcctt ggctttgtaa agagctttat cggcgcgatg aaagagccct tcaaaagtat
    26041 cctgttcctg aattccgaac tcagtcactc ccatgctgat cgtcaccttg aatttgaggg
    26101 tatcaacatt gataacggtt gaagcaaggg cttcacgcag acgctctgcc aggtgaagcg
    26161 cggtcttttg cgaagtttca ggcagcaaca gagcaaactc ctcgccgccc aatcttgcaa
    26221 aagtatcttc ttttcggatg tgatttatgc agatattccg aagcgcgatg agggcatggt
    26281 ccccgctggc atggccgtag gtgtcattca cctttttgaa gaaatccatg tccatgagaa
    26341 ccagggacaa ttttctttta taccgaagcg cacgccgaat ttccttgcgg gccaggctca
    26401 taaaataacc gcgattggca gcacccgtca gcgaatcact gttggccagc aaatgcaatt
    26461 cactttgcac cttcttaagc ttggtgatat cgtgcaggat gatcaaacgt ccgatatttc
    26521 tgcccttgtc atccacaatg aacgatgtct gcatatccac gtaacgtttt gtcttccgcc
    26581 ttatttccat ttcaatttgc ggcagggtgt tcttttcata atgctgccat ttcttaatca
    26641 cctcctccac cggcttgcca attttatctg agttgatgtt gaggaggttt tgtgcagcgg
    26701 ggttcatatc gacaatacgg ttgtgagaat ctatgaccag aacaccatca cgcatttttt
    26761 cgatcaaaat atcacgcgcc acggggatca aatcaagcag gtgatatcgg ctcacggcaa
    26821 aggcaaaagc cacagcggca acggtaaagg aaaaaggcgt attatcggcg ccaggcagtg
    26881 gattcaaccc aacgataaag acgatactgt tgagccaggt cacaccaatt cccaccgcaa
    26941 caagccccaa ttgttttcgg taaatcccag aggcccgaat aaaaccaccg acaatcaatg
    27001 ccgtagcgac caatatcaag gtgtaggaat aggccacatt cgcccaaaaa acaggcccgg
    27061 cgtccagaat gaccgcactg ttttcagcac gtttccctgc aaaaaagaga ccatgatatg
    27121 gatcggtaaa caggcaggtc aatacgagga gcggttcaag atacagaatt aaagtaaaag
    27181 gccgtttcag ccattcctgc cgatttgtga tctgtaaagc aaaggaaaac aatgcccccg
    27241 gaacgatcac tgcgccaaca taggtaatat caagccagaa atattcggtc ggccccggag
    27301 tccctgccca aaagaggcca taggtggcat cccaccaggc aagggccagc aacagagcga
    27361 tcaacgagtt tgcacccgcc gcatttttcc gcgaccgcga aacaatcaca gccacgaaca
    27421 aacaggtaat gctagcaatg agaaggaatg aactgtacgt aatgcttgga ggggataatt
    27481 gcatgagcaa ccttgcccct attgtacatc tttgcacgtt caaaaaacat gagtagttta
    27541 ttcttggaga taaacacagg gcagttgctt taacacaact gccctgccca catttctgat
    27601 taatggtgat ttggttgcag ctatttttca gcctgccttt tcaatgcaga gacaaaaccc
    27661 tcaaagttcg aagttagatc gggaatattc ctgccaaaga gcggcagcaa cagaccactg
    27721 aatatctctt cggtgtgaaa atccgtccct ccatcttttg acggagtcag tgtgtgggtg
    27781 cgctcggatt taaacaatcc caacggcagg ccgccggtca ataccatctt tttaccgggc
    27841 tcgaagaccg tcaccttcac cggaaaggat tgggcgctca gcttggtaaa aaatgtcact
    27901 gtttcgccca gagccagcct gccatcaatg tggtccatgc ttaaatccca ggctgggtaa
    27961 ctacttgcat ctgaaagaat cttccagatt ttttcaggcg aggcattgat cgaaatggat
    28021 gcgcgatatg atttcatttg taaagtctcc tatttttcgt tcttcataat ctgtttaatg
    28081 tagtctttca acgcggcttt attgaaatcc atcgacgcaa tccgcatcgt ccgtgtttta
    28141 ctgccatcac cttccaacaa gccgtattta tcatggagag aggctccttc aaaaaagatc
    28201 aaagaaaccc tgcctttccc aaccacggtt tgaatatggt tctttgttgt tccatataca
    28261 aggcagttct tccatttgat actttcatta agaacaggcc ccgctgccag aacacaagcc
    28321 cgtatctcca aaaagagcgg agcgatttca acatccagct cgccaatgta ctcattcaca
    28381 atatcatttt ttgatttggc gtgtttcaca ataatctcct ttgagcattt ttcatatccc
    28441 tattttatcc acggcaggca aaacctcatt gtacgaagcg gacatcaggt tatcctgcaa
    28501 gatcgaactg ttttgaaaat actccacagg cgcaagtccg gtcatctttt tgaacgaacg
    28561 attcaaatgc ggctggtcat aaaacacatc cggcgtcaca tgctcaatga tacgcggatt
    28621 ccgtgccgaa aaatcgcaga cctgccccag gaacacatga acgttttgga tgttctgcag
    28681 gtcttttcta ctcaacccaa acactttttg atacaaacgc cgtttatgcc ttgccgaaaa
    28741 attatccaac caatccgatt ctttgaaatt cagcgacaat atgggatgtg gcaatttcgg
    28801 gtgctgctgc attcgcacat gttccatcac ctgcagtgta aacgcttcaa ggtcgttcgt
    28861 gtctcgctta acccaatcct gctttaaaaa acaattggct tttatctccc cggaaaaaga
    28921 ctcggcgaac tttagcgaaa aacgcgcacc aaacaaaacc agtggtgtcg aaaaaaacag
    28981 ctttacaggg cgtgtgtaaa ggttccgcag tgactgatga ggcagggctg attttctccc
    29041 cgctccgcca tccactatgt gaacctgtcc tttttggata aaaagataat cgaagaatgg
    29101 ctccggtata aagtgctcca cactcatcac cgacatggga gactccatcg tcattttcca
    29161 gaaaaataaa attccaaggg agggatctcc cattttcttt tgaaaagaaa tcttcatcgg
    29221 tgttccaact catccccgaa ttcaacaaat ggatgcaggc ttttctcttc caactggtag
    29281 ttggtcacct tgcgcattcc accgctccct gtagcatgga tcggtgcttt ggatatttcc
    29341 gccatagtgg accagcctgc aagagaaacc acaagcggtg atggtgatgc ggtcaactgg
    29401 tccatgtcca ctggcgcaag cacacaccaa atgaccaggt cagctttttt ctttagagtc
    29461 ggatattgat ccacagcaat acagcggccc aattcagccc agtaacttgc gctccaggtc
    29521 tttacctcaa gcctgcacac ctgcgaataa tttttgacct tcacaacgat atcgcacaag
    29581 ttctcacgct ccgcaaaacg gaaatgcgaa tcgagtttga atctgttttc gagcagatat
    29641 ttctccgttg caagctcgcc cagtttcccc gtcaggtggg aggcgaggcg gttgttgtaa
    29701 tatccatcgc gttgtgacca tttctcaatg gaggcatggg ctgccgatgc agcccatctc
    29761 acatcagcgg catccaattc aacctgaagg gctttttgcg cttccagatc catcgcagat
    29821 tccaagcccg ggttattttg tttcttccac gacctgacgg atcaggtcca cgatcagggc
    29881 gggttgttcg agttgaacaa aatgcccgct attctctaca acaacaagcc gcccgcgcga
    29941 agaaaagctg gcaagataat cctgggtggt gtcccaaatt tcgtctggtg gataatcgtc
    30001 ggcgggacga atgcccgctt tgacaacgac cagcggcagg tcggcgagag aaacgaggcc
    30061 ctgggtctgg attccgcttt gttccatggc agaatattcc ccggcgacag cgctccacat
    30121 gccatcgtcg taataggcgg ctttgtaggc aggctgaagt tcttcaggca atagaccggc
    30181 ttgcagcatt tcactcaacg ggaggatccc caattcaccc acgaggcgca cctgccccaa
    30241 tggtgcgatc gctttcaagg tctgcgcaag tggaagtgac tgcgtgatga catccggcac
    30301 agggatgaac aggtccggac tccatgtttc gatgagcacc atgcccacaa cttcatcggg
    30361 ataattattt tgatacaaca aggcggctgg tccccaaaaa gaatgggcca cataaacaaa
    30421 tgggccagtt tcacctgagt ttctcaacaa ggtatgcaat tccttgacga tcacctctgc
    30481 agtgcgcgga tttggccccg cctcactcca cccataccct gccctgtcgt aggagcagac
    30541 tcttgtaaat tttgatatct ccggctgaac cttctgccag tgaatggacc attcaggaac
    30601 gccgccttcc aaaatgacgg ttgggctccc ctcgcctgtg caatagatat gcatcatatg
    30661 cccatctaca ttggtcaatt gaccaagcgg cagattttgt cttccttcca ctctctgggc
    30721 aatcacctgc cacacccacc cgatcggcaa ggggatgatc aacaagatca gcaatatcct
    30781 tccgatgcgt gaaagccagt aacccaaatt ctttcgttga tgcgttgttg tcatatggtc
    30841 tccttgcagc cagggcactt acaaaaattt aaacaatggg attatatctc ccacatcaga
    30901 ctttcattaa cctccccctt cgtccagttg cgaatttggt cttcctgtct tatagtccgc
    30961 tggtctgctg gtcaaagtgt ttactgccca acaaccagca gacccaggac aggagactat
    31021 ttttatgcac ggatacggct ggttcatggc catgcgtcag ggcgacgaaa agcccaaagt
    31081 gactcgcgag cttttattgc gcgttctcaa ttacgccaaa ccctactggt ggcatattac
    31141 aggcatgctt gtaacgatcc tgcttagcac gggtctatct ctgctgacac cgctcatctt
    31201 ccgcaacatg atcgatgtgg tgttgcccgc caaagacacc cagaggctca tctggctggg
    31261 gctggcgttg ttgttcatcc ccgcactcaa cggagccatc agcgtcgttc aacggcggct
    31321 caactccact gtgggtgaag gtgtgatcta tgacctgcgc gccagtttgt tctcgcgcct
    31381 gcagcggatg tcactgcgtt tcttcaccaa caccaaaaca ggcgaactca tgtcacgcct
    31441 caacagcgac gtggtcggcg cgcagaatgc catcagcaac accatcgtca acatcgtcac
    31501 caacttgatc gaagccattg cattattcgc agtgatgatc tcgcttgaat ggcgcttgac
    31561 ccttgtcagc attttgatcc tgccgctttt tatcatcaca gcccagcggc tgggaacggt
    31621 actgcgcgat atcgcccgac aggcaatgga tacaaacgcc caaatgaacg cccacatgaa
    31681 cgagaccctc aatatcggcg gcgcactgct cgttaaactg tttgggcgca cacgggaaga
    31741 ggaaaaacga ttccgcgaac gcgctgccaa tgtccgcgat attggcattc gccgcgctgt
    31801 gatcggctca accttctttg tcatctttgg gttggtcacc gcagttggca cggcgctggt
    31861 ttacgggttg ggcggctact tcgtcatcac agatgtcttc accgtgggca ccatcgtcgc
    31921 ttttggttcg taccttggtc aactgtacgg taccctgcaa ggcctcgcca gtgcccctgt
    31981 ggatttttca acatccatgg tcagcttcga acgcgtcttt gaaatcgtgg accttccgca
    32041 ggatattgcc gaaaaagaaa acgcgattga actgcaagat gtgcaaggcg aacttgaatt
    32101 caaagacgtc accttcaact acaaagtgga cgagtcgatc ctgctcaagg atgttaaacg
    32161 ttatggcagc atggataatg taaaagcggt catcagcgga caaccgtctg tggatgggaa
    32221 aaactcacag ggaggaaacg gttcttcgtc cagaatcaag aatcgggagg aaaggaacgc
    32281 cgcttccgac tcacccgagc ctgattccac atcccaagcc agagacatcg ccctcgaaaa
    32341 tatctccttc gtcgcccgcc ctggacagtt gatcgccctt gttggcccat cgggcgcagg
    32401 caaaaccacg gtgacctatc tcattccacg cctgtacgat cccacagatg gcatcatccg
    32461 cattgacggt cacaacctga aggatgtgac tcttgattcg ctggctcatg ccatcggcat
    32521 ggtaacgcag gagacctatc tctttcacga taccatccga accaatctga cctatgccaa
    32581 acccgaagcc acccaggcag agatcgaatc cgctgccaag gcagccaaca tccacaattt
    32641 catcatggac ctgcccgaag gctacgacac catcgtcggc gaacgcggct accgactcag
    32701 cggcggcgaa aaacaacgca ttgcgcttgc gcgtgtcatt ttgaaaaatc cacgcattct
    32761 tgttttggat gaagccacca gttcactcga tagcgaatct gaagcgctga tccaggatgc
    32821 gcttaaacgc gtgatggctg gcaggaccag cattgtcatt gcacatcgtt tatccaccat
    32881 ccttgcggcg gatctcatcc tggtgatgga tcgcggcaaa atagccgaac gcggcacaca
    32941 tgaagagttg cttgccgctg gcggcattta cagtcaattg tacgaaacgc aattcagggg
    33001 agagcgggtt taaattgctg tggagcattg gcaggcgatt attcagcctg ccaatttctc
    33061 ccacagttga cgtgtgatct gcaaatgccc aagatgaagt gtggaatgct ccaaagcatg
    33121 gagcagcgcc caggccacgg tacattctcc ctttgaagat acacacagtc tatcaaggtc
    33181 ttgtaaagag agtctttcaa gggtggaggc ggcgtaatca aaactgtcat caagccgctt
    33241 tttcaggaaa tccattccca acccacggac tttgaattcc gctgcgcggt cacggttcga
    33301 tgactcgccg actgccacat caccgatcca atatcgttcg gcaccggtaa ggtggtacac
    33361 caaaacacag atcgaattca tctcctgccc ggggccccaa tccaacgcct ctgcgggcag
    33421 gtcgttcaac acggcatgga tatcgttgtg acatttttgt aacaggttca ggtaatcaga
    33481 aaaaaagggg tgcatctcat tgctccattc caatgtattg atagatttct tccgcttttg
    33541 cggtcagcca gccttccaat tcagggatct gctcgcttaa ccttgcgcct gccacgatcg
    33601 gcagccagcg gatatattcc gccctgccct gcggacgaag gcgaaaatat tcacgcagat
    33661 agattgagtg aaagacccgc actaaaaatt tcaacgccgg gttgtgcatt tccctgtttt
    33721 cgacaatgcc aagcaggata atactggtcc gcgccacatc ggcaagtgga ttaccgcgtg
    33781 aggtgtccat ccaatcaatg atgacggggt ctgattctgt cagcaacaca ttgtcaaaat
    33841 gaaaatcgcc atgacagaca cagtcacttt cggggaggga atgcaaaacg gtcaacaaag
    33901 cagttttgag cggagtcgga agcgcaggtg cattttcgat cttgcgctgc aattttccgc
    33961 gtagtttggg catatccgca ccgaaaacaa aatcatgcat ccgagcatgg agttgggcaa
    34021 gggttttggc gcaggcaaat aacttccacg gtttatgttg aagcatggcc gccatcgact
    34081 cgccgtttac acgatcataa accagcccat tgcggccatt cacccgaatc aattcatgcg
    34141 ccacaggaga ttttatgccg cttgcatgca cggcccgcgc aatgcgcaat tcatattcta
    34201 tgtcttcaag cgggaaccag tgatgaaaca atttgagaac atagccatca tcccatttgt
    34261 aaacatcggc ggtacgtcct cgggccagtg ggtggtcaag agaaatgtgt ttcatactct
    34321 tgcgttcatt atagggtatt ttgacaacgc ttgacaactt actgactagt aagtaaaata
    34381 cggtcacaat ggtcaaaatt gagaactcca aaaagacgat tctgaatctg gcggaggccc
    34441 tgttgcaaga caagggtttc aacggtttta gttatgcaca tattgcatcg gaacttggtg
    34501 taaaaaatgc cgccattcat tatcacttcc ctacaaagga agacctgggg caggcggtga
    34561 tccaacgcta ccgcgaccgc ttcaaattat gggtcaacaa ttcccgtgtc aaagaccttg
    34621 ctccacagga aaagctggat tggtttttca gcatttacac cgccatgcgt tccgatcagg
    34681 gcaaggtctg tctggttggt tcaatggaag ttgagttcaa ctcgatcccc gaaggtttgc
    34741 aggcggaagt tcaagccatg cataaagaat tgctggcctg gcttgaaggt acgttgaccg
    34801 aaggcagggc ggcaggtgtg tttcgcttca acggtgaacc agccaacaag gccgcattga
    34861 tcctctccac tttgcaggga gggttgcaga tggcccgcgc gctgggaaca aaaaagttcc
    34921 gcgatgtcat cgagcagctt aaattggatc tgctcgctta atttttttat catcaaaact
    34981 tactggttag taagtaaatc acaaggagaa tcaatgacaa ctttggaatc aaccgcaaca
    35041 aaatatcagg ggatcgtgaa gaaggcgtat caggcatttt tcagtccatc tcaatacgaa
    35101 gtgaaatccg cagaccgtga gatcatcgca cgcggaaaca attaccgcat ctcttttgaa
    35161 ggcggcgaat tggcggtcac cacctggggc gaaagcggcc ccgctgtttt gctgatgcac
    35221 ggctggggcg gcgcccgcgc gcaaatgacg ggctttgtcg aacctttgct ggctgcggga
    35281 tatcgtgtcg tggcatacga ccagccagca catggtgaat ccgatggaaa gatgacgaac
    35341 atccttgaga tcacccccac catggatctg gtcgaaaagc gtgaaggaaa attcgatgcg
    35401 atcatcgccc attcgttcgg gactctgatc acatcttatg cactggtcca tcggaatttt
    35461 ccgcccccca ccagactggt gtatctgggc gctttcaatc ggcttttgga ttcccttccg
    35521 cgttttcaag tgcttgcaaa cctgcccgat gagatcatcg aaggtatgcg aaacatgatc
    35581 tacgataatt ttggaagagg cgaactggac tccatcatca atgagtccat ggtgcagcag
    35641 atacatattc ctgcattaat gttccacgat gcttcggata acgtcacccc cgtggatgac
    35701 agccgcgcca ttgccagtac ctggaaatct gcgcaattca tcgagacctc agggctggga
    35761 catcgaggtg cgctgcaatc aaaggaagtc cacgcgcaga ttgtggattt tttacagaaa
    35821 aagtaatctg atttccaccc aagaaacaaa gccgcaaagt ggcggggaat ttttccctgt
    35881 cactttgcgg ctttgcacaa gaaatacaag tacaatccct tcagccaaat gaccaaaagg
    35941 gatgagctta tgaaaactgc gttgatcttc ttctttgccg ttgttagtga agtgacagcc
    36001 actacagccc tgaaattttc tgaaggattt acaaaattcg tccccagcct gatcgttgtt
    36061 gtgggatacg gcctatcctt ctatttgctt tcgctcagct tgaaggttat tcccatcggc
    36121 acggcatacg cgctttggtc tggaatcggc atcgtgttga ctgttattgt cggcattgtg
    36181 ttctggcgcg aaaccatgga ctgggcacgc ggcatcggca tttttctgat catgttgggc
    36241 attttggtga tcaacctgtt ctcaaactca acagctcatt aaaggtctga cgaagcacat
    36301 tgccgctgtt ttatgtcaat accaaaatcg gacaaggtgg gttataaatg cctatcttcg
    36361 tgaaaaagga taaatgcatg aacttacgcg accttctcag aatcaccagc cgcacatttg
    36421 ccatcggtat cgaacgcctg cctcgtgttt tatgtgatgc cacgacgatc gcctatctgc
    36481 tcttgcgagt ctctgattat cttgaagata atgaagaaat ggaagccgac aagaaggtgg
    36541 aacttttgaa tctttgggtg aacattctgc gggaggagga ggcggtcgaa accctgacca
    36601 ttcaattgac cgaagccaat actgaaaatc ccgatgcgat cgtggcgcaa cactcaaaag
    36661 agatcctgca gcatctacgg tcattgccct acgaagtaca ggagatcatc gttcatcatg
    36721 tcattaacag cacccaaggc atggcgcgct ggacacaaca cggatcacac ctgaccgatg
    36781 aaaaagacct cgacgattac atgttcgagg ttgcaggccg tgtcggctac ctggtgacac
    36841 agttattcgc atggtattcc atcgccattc gcatcaagga aaaagagatc atgccgctcg
    36901 cacgcgagtt tggactgggg ttgcaaacgg tcaatgtgat tcgcggcctg cgcgaagact
    36961 tcaaccgcgg ctgggtctat gtgccgcaaa agttcctctc cgaaatggga ctgacaaaag
    37021 agcagttgtt cgacccgcaa cacagtgaac aggcgctgaa agtattggac atgatgaccg
    37081 acaaggccga gcgtcatttg cgaaacgccc tgcaatatgt caaatcactg ccatggtggc
    37141 tgcatggcat tcggctgggg tgcatcttcc cgctgatgtt cgccatccgc acactggcgg
    37201 tctgccgcca taacactcag gtcttcgaaa gcgaagccaa gatgacccgc aatgaggtta
    37261 aaaagatcgt catggattcc actctgtggg gttggtccaa tctctggctg gatcggtatt
    37321 atcaggaatt gaatgtggtg ggggaatgac ccaaaccatt cagcgttcaa tctcgcgcaa
    37381 aatggtcggg aatctccttg acataattca gcggctgaat tatgattgag gtcaataaag
    37441 tctattggat tactaggagt tatagataaa gtgagacttt ccaagcgcgg cgagtacggc
    37501 cttcgggcaa tgatcgctct ggcagaaccc gctgaaaaag gcggcgcccc acagatgatg
    37561 cagatcaaag agatctctct tcgtgagcag atctcgccca aattccttga gcagatcctt
    37621 ctcacactca agaatgcagg gctgcttcac agcaaaatgg gcgtcggcgg cgggtattac
    37681 ctggccaggt cagccagtga gatcaccctt gggcagatct tccgcgtatt ggatggcccc
    37741 gtggcaccga tcaagtgcgt aagccagatg gcatacgaac catgcggctg ccccgatgaa
    37801 gagacttgcg gtctgcgcct cgcaatgggc gatgtgcgca atgccatagc cgatattctg
    37861 gataacacca gcctggctga tgtaaccaaa cggcaaagca cggtccgcaa aaagctcggc
    37921 atcaagtaaa gtgaatgaaa ctgcgtgcgg gggaacgcat ttttatttac ccaaaaagta
    37981 tactagtact atagacttta taatcattat gatctgtata ggagaaacct tatgaggaaa
    38041 caagttttaa catggctctc tgtgtttttg gttttggcgg tcactcttac agcgtgtgga
    38101 actgctgcgt ctgaggcaac agccgtccct cagcctgaga cgggcacttc atccgaagaa
    38161 ccgctcagcg gaaccatttc catctccggc gctttcgccc tctaccccat gatgaccgtc
    38221 tgggctgaag aattcacaaa gcttcaccct gatgtcgaat tcgacgtaca aggcggcggt
    38281 gcagggaaag gcatgaccga caccatcgcg ggcgcagtgg atattggcat gatctcccgc
    38341 tccatcaagg aagaggaaac cgcacaggga atcttctggg tctctgtgac aaaagatgcg
    38401 gtcttcccga tcattaatac cgaaaacccg gtggccgccc aaattct
//
LOCUS       DAOQLC010000026        38054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_308_length_38054_cov_23.139898, whole genome shotgun sequence.
ACCESSION   DAOQLC010000026 DAOQLC010000000
VERSION     DAOQLC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 38054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_38054_cov_23.139898"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PL000_06570"
     CDS             <1..918
                     /locus_tag="PL000_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNC88596.1"
                     /translation="FFSILSISWGILFSDKRGKIRPMKLNLALAQINTRLGDVEANLA
                     KHLEYIEQAKAEKTDLLVFPELSLTGYVLQDLVASAAHKPTEDDPIFKHLLKASHDLD
                     LVIGFVDEDSRHRFYIASAYLSGGKAIHVHHKVYLPTYGLFDEGRFFAWGDSVRAFNT
                     RFGRMGMLICEDFWHASPPYLLWLDGADIMLFSSASPGRGLNDKEKLESARWVERVTK
                     AYASMFTSFVAHANRVGYEDGLHFWGGATAFDPNGEELAHGPYNEEAITYTQLDLNQL
                     RRTRARLPLLRDERTALIRNELDRILSRD"
     gene            911..1738
                     /locus_tag="PL000_06575"
     CDS             911..1738
                     /locus_tag="PL000_06575"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNC88597.1"
                     /translation="MTDLNLTINADLAREILTGFIKSEITRVGLTRAVVGLSGGVDSA
                     LSCALAAEALGPQNVLAVRMPYKASSKDSLEHAQLLIDQLGVQSKTIEITDMVEPLFK
                     LHPDISNMRKGNIMARERMIVLYDQSEVFKGLVVGTSNKTEILLGYSTLFGDSANALN
                     PIGDLFKTQVRQLSRAMNIPAPILEKPPSADLWADQTDEKELGFTYEEADKLLYLLVD
                     QRYSPQEAIEVGFAEDFVKTVTTRIRRNQFKRMQPPIAKVSNRTIGYDFLYLRDWGT"
     gene            1812..3098
                     /locus_tag="PL000_06580"
     CDS             1812..3098
                     /locus_tag="PL000_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88598.1"
                     /translation="MHIPPSLKHKKFFYLWLGQLISITGTQMQLWALFWHVSNLDKNP
                     IALGGIGLARILPVIVFSLIGGALADSVDRRKVMFVTQSAAALLALTLGLLTQFGQVT
                     IWYIYVITAIQAIAIAFDGPSRQALIPNLVPKEDLPNAFSMTFTSFQFGSVLGPALSG
                     FVIAWAGQEAVYYFNAISFGAVLFALFMIGDVPQSFAEKSAGISMDAIREGIRFILGK
                     PIILSSMLLDFVATFFASANTLMPIIATTVLKVGVVEYGYLSAASAVGAVTVALAISQ
                     VKVIRRQGPMFMGAVIVFGIATVIFGLTRSFWVAWLAIATTGAADGVSTIIRNTIRQL
                     QTPDHIRGRMTSINQIFFQGGPQLGEMEAGSVAQFFGASFAVISGGIGCIIGTLLIMW
                     KWPQLMHYDGHEPIEGEIAPSPSSGASIVSNRVRRP"
     gene            complement(3129..3983)
                     /locus_tag="PL000_06585"
     CDS             complement(3129..3983)
                     /locus_tag="PL000_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88599.1"
                     /translation="MDQFETLTQINLDDLVSSFGWQNHPLAAKILRFLFRKPAETFAR
                     HMMEYDDTTGMHGIAEGGRNLLNRYVKELRVLHADRVPDSAFLALSNHPGMTDTVALF
                     AALNRKDLHIIALDRPFLNALPHTTRQLFYVHDDPAKRMTLVRQVSSHLKNGGAALTF
                     PAGHIEPDPEVYPGAVDSLQAWTDSVGVFVRMAPEAAILPVLVRGVVSAKYADHWLLK
                     IKKTQEEKEKLATALQLLGMVMFNEKPVIVTVQIGNPIYVKALGTNDAAAIHQAVLAE
                     MKNLIESS"
     gene            complement(3985..4581)
                     /locus_tag="PL000_06590"
     CDS             complement(3985..4581)
                     /locus_tag="PL000_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC88600.1"
                     /translation="MAGMTRGKGLLEPMLADLRAQRANKLIPSNLRNGRILDIGCGSF
                     PYFLAHTAFAEKFAIDQIPLPQQTASELKIESFTLDLEIEPRLPFENDYFESVTLLAV
                     VEHLDPALMAKLFKEIYRVLKPEGMVILTTPAAWSDGLLKFMARIGLVSAEEIHEHAY
                     AYTLPLLGWYFGQAGFEMTKTKFGYFEFMLNMWATAKK"
     gene            complement(4877..5182)
                     /locus_tag="PL000_06595"
     CDS             complement(4877..5182)
                     /locus_tag="PL000_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNC88601.1"
                     /translation="MNNQIAPEIEIIEITETELEPLYRILIHNDDVTPMDFVVHALTT
                     FFYLGTPTAAEIMLKAHITGMAYVQTVAKSEAERRINKAHFAAGLEGYPLHFSMEPE"
     gene            complement(5199..6020)
                     /locus_tag="PL000_06600"
     CDS             complement(5199..6020)
                     /locus_tag="PL000_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC88602.1"
                     /translation="MKFETLPNLTLPKIGFGTWKIGGESTPDPSLDSVSMAALRSALE
                     VGYTHFDTAEMYSSGHAEELIGRVVRETKTKREDLFITTKVSPEHLNPKDVFSSCEKS
                     LRRLQMEYVDLYLIHWPSTGMKLEETFTALNKLVRDGKVRHLGVSNFKLRDLKKSQEL
                     SETPLLTNQVPYRLPDRAYVENGVVEYCQKNDILITAYSPVKFRNLPVNKTLQEIAEA
                     HSATAYQIALAWLVSQPRVITIPMSFNPQHILENFEAAEIVLTSQEVEQLNRLYI"
     gene            complement(6028..6915)
                     /locus_tag="PL000_06605"
     CDS             complement(6028..6915)
                     /locus_tag="PL000_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNC88603.1"
                     /translation="MTNKITINIINAFTDAGEGGNPAGVVYPADEFTKEDKQKIAAKV
                     GLSETAFVSRSQSAEFKLDFFTPNKQIAHCGHATIATFSYLSQQGIVKGPKSSKETID
                     GNRDIYLKGEVAYMQQLAPKYFPLPYKGISEQDVLASLHLQKTDLLAGHNPMPVNTGN
                     SFMMIPVKDEATLKAIHPDLNAIHQISEALDLIGYYPFTLETQVRGRDAATRMFAPYY
                     GIPEEAATGMAAGPLACYLFDQININKETLILEQGRLMPTPSPSEILVELKLEDGSIK
                     SLLAGGRAKVTRSIEIELD"
     gene            7026..8534
                     /locus_tag="PL000_06610"
     CDS             7026..8534
                     /locus_tag="PL000_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC88604.1"
                     /translation="MQNMLKNKRNWWIAAILVVAGVLVWAGVAATGSANAAEAQTEQV
                     IAMELAQTVETSGTLESQPFASLNWKTGGIVESVHVQPGDFVKAGDVLLTLQPESTSA
                     SLASAQADLETAQANLRALTFPDGSSVGEARENASSAFDAWSDARVDLMDALAYNRGG
                     GDDDLYSDVVTARDDLVNALEEYPLAANVDAQFYYWAARADALGYHGEYDHAALVPVF
                     RAELNADDAELVDEILTAQTEFETLAKAFAESMEDQDDAVDAMKAFGAYEQSADALLD
                     AMEAEYGVLVQPSESDLASAQAKVDAAQASVNNLQIVAPFDGQVLSVDDLVGDTVSAG
                     DLSVNMADMNNLYVEAQVDESDVANVRVGNQVEVTLDALPDLVLTGSVTVVNPVGEVV
                     GGLVKYSIRVELDPVKDDFLPLGATANVVIKVSDTQASLAVPIMAIQNDNEGEYVWVL
                     RDGEAVRVNVTGGALVDGYVVVTGDLQEGESLQLVERESGFTPPNPFSGGAK"
     gene            8531..9247
                     /locus_tag="PL000_06615"
     CDS             8531..9247
                     /locus_tag="PL000_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88605.1"
                     /translation="MIQADGLTKVYQMGDSEVRALDGASFTIKKGEMVAIMGPSGSGK
                     STLMSIIGCLDVPSEGSYSLDGEAVQTLDETKLADVRGRKIGFVFQQFNLLARTSALE
                     NVMLPLTYAGISGKEREDRAKKALERVGLGGRTSHAPNELSGGQQQRVAIARAIVNEP
                     SILLADEPTGALDSKTGVEIMDLFQRLHRENGQTVILVTHDSYVARHTERIIKISDGR
                     IVSDEVNPNPIKAGTGRGEE"
     gene            9250..10488
                     /locus_tag="PL000_06620"
     CDS             9250..10488
                     /locus_tag="PL000_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88606.1"
                     /translation="MLYSENLKIALRALRANKMRSVLTILGIVIGVATVVALLSIGNG
                     ATASITDQIQSGGSNLLTLSPGRMQTPMSSLGQSQQASYLYYSDYETLSKTLTGEITS
                     IVPVYQASYTVKFGDESFSTSVSGVMEDHAQARSFTISAGRFVTDGDNDALARVAVLG
                     ATVAEDLFGTLSPIGQTISLNGVKFEVVGLLESEGASIGSPDDIIFVPLETGYIKLFG
                     DTANHNSKKTVNSILISVTDSKDMDAISAQAEYILRRAHELKPTEEIDFNIMSQADTL
                     ETLNSVTQTLTVFLGAIAAISLLVGGIGIMNIMLVSVTERTKEIGLRKAVGATKNQIL
                     TQFLIETVTLSLLGGLVGILLGVGIALAFSLSGLINSVITADSILLAFFFALAIGIFF
                     GLYPAQRAASLHPMVALRYE"
     gene            10599..11264
                     /locus_tag="PL000_06625"
     CDS             10599..11264
                     /locus_tag="PL000_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88607.1"
                     /translation="MPKKILIVDDEPQIVEICRDYLKAAGYETVTASNGSQALALARR
                     EKPDLIVLDLMMPEMDGLDVTREIRRASDVPIIMLTARVEETDKLIGLELGADDYITK
                     PFSPRELVARIRVVLRRVVNSPDGDTIRAGSVELDRAHYEVHLPDKTVSLTATEFEIL
                     ATLMSQPGRIFSRAQLLTATHGVTFESLERAIDSHVRNLRRKLEPADLIVTVHGVGYK
                     FEG"
     gene            11266..12597
                     /locus_tag="PL000_06630"
     CDS             11266..12597
                     /locus_tag="PL000_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88608.1"
                     /translation="MHSSVHEIRTRLLSLLVRAFVFVLFLALIFFLAVVGAVLTSQTS
                     PIPIPFGRALEGYYIGHGNWDGVEKVFDTLEELKTLDVLLLDEGQRIVLDHDSDPASV
                     VGSSYEIRPNDFLINLTVEGQVVGRLAFTSITPWSRLGIARAVVIPVGIISLLLSIFL
                     LIVATVLVRRFVNPLADVIYAAREVTNGKLTTRISSEGPQDMRSLSESFNEMTAALER
                     NDRERRDLLADIAHELRTPLSVIRGRLEGIVDGIYTADDGQVSLALEQTYLLERLVDD
                     LRLLTLAETRQLHFEKKKVDFVQLSQHTIEMFSAEAREKNISISLEMGKGNYTAELDP
                     QRMEQVIGNLIGNSLRYIPEGSKIWLALEETSKEVKLFVNDNGPGVAQDELPYLFDRF
                     WRKDKSRTRSAGGTGLGLAIAKQLVEAQGGTIHASNLREGGLQIAIEFRKM"
     gene            complement(12584..13756)
                     /locus_tag="PL000_06635"
     CDS             complement(12584..13756)
                     /locus_tag="PL000_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNC88609.1"
                     /translation="MEDISLVFIELGAAIIGLALLTRLASRLGLSAIPFYLLAGLAFG
                     NGGLLPIQFSEDFVHIGAEIGVILLLFMLGLEYSGEELGTNLRSGFPAGIVDFLLNFP
                     PGLILGFVLGWSPLAAVLLGGVTYISSSGVIAKVLSELGRMNNRETPTIISILVLEDL
                     IMAVYLPIVAVLLIGQEISQAVLSVSIALATVGTVLFVAVKYGKKFSKLIAHQSDEVI
                     LLTTFGLMLFVAGMAQYLQVSAAIGAFLMGIAISDSIAERTHKLLGPLRDLFAAIFFL
                     FFGLQIDPASLPPVLLLAVSLGLFTTLTKILTGWWAARRAKSNPLEAFRAGAALVARG
                     EFSIVIAGLGVSAGLEPQLGALSAAYVLFLAILGPILARFIEPIYRAIHKKQITSS"
     gene            complement(13808..14206)
                     /locus_tag="PL000_06640"
     CDS             complement(13808..14206)
                     /locus_tag="PL000_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC88610.1"
                     /translation="MDKRINISSGAKWEDIVGYSRAVRVGNMIEVAGTTAVDERGEIV
                     GLNDPYEQTKYALAKIEKALIEAGASMQDVVRTRMFVTDISKWEEVGRAHGEFFRTVK
                     PAASMIEVKGLIDPKLLVEIEVTAIIAEAK"
     gene            complement(14266..14649)
                     /locus_tag="PL000_06645"
     CDS             complement(14266..14649)
                     /locus_tag="PL000_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC88611.1"
                     /translation="MSTYPIDILNEYCPSRKVMELIGDKWTLLTLNAIARGINRHNAL
                     LREVGGISQKMLSQTLRALERNGIVTRHVFPVVPPRVEYELTLLGKSLNEPVVALGKW
                     AQEHYAQVEKAREKYDIEFKNDEEE"
     gene            14749..15078
                     /locus_tag="PL000_06650"
     CDS             14749..15078
                     /locus_tag="PL000_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNC88612.1"
                     /translation="MNTWLTAIHIKPEHVEKFLAASTPFAGESLRESGLVSFALLQRV
                     DDIAQFSMFDVYHDAAARENHLTSAHYLAWLTIVTPLLAAPMTSLPFNPIFPPPEDWE
                     RHLETES"
     gene            15131..15568
                     /locus_tag="PL000_06655"
     CDS             15131..15568
                     /locus_tag="PL000_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC88613.1"
                     /translation="MEQKEVALSVSKAILNGEWDKLNGILSDDFTYTGDGMFLNKAQY
                     MDFMKGMRAAFSNMNFEFSHVISEGDAVSIRFVVTAKHTGSFMGGAATNKNLNFSGIF
                     IRKVKGDKVVQEWQATDLLGMMTQMGVGTLLGYAIFGVWLKKK"
     assembly_gap    15639..15738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15761..16321)
                     /locus_tag="PL000_06660"
     CDS             complement(15761..16321)
                     /locus_tag="PL000_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNC88614.1"
                     /translation="MQKEIIIAGFGGQGVLFGGQVLAYAAMDTGRDVTWIPSYGPEMR
                     GGTANCTVVIADHEIGSPLVKNPPLAIALNLPSFDKYEPMLASGGTLIVNQSMVDHSA
                     RRSDIHTIFVPCNEIAEEIGDKKLMNMVAIGAMLTALPEIKIEDVEKALEAHLPARHK
                     HLLPKNYEALKRGFEHAQKEWDKVPA"
     gene            complement(16393..17160)
                     /locus_tag="PL000_06665"
     CDS             complement(16393..17160)
                     /locus_tag="PL000_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC88615.1"
                     /translation="MLAENVVCVRPEGFTESPTHYCPGCTHGVAHRLVAEVLQEMNVL
                     DKTIGVAPVGCSVFAYNYFDTDFVEAPHGRAPAMATGIKRVLPDRIVFTYQGDGDLAS
                     IGMGEIVHAAARGENLTVIFVNNANFGMTGGQMAPTTLPGMKTTSSQNGRDVETQGYP
                     IKMAEMLATLDGVGFCARRSLHDAKNIRLAKKAIRMAFETQARGLGFAMVELLSTCPT
                     NWGMTPVNSLKFVENSMVPAYPLGDYKISEAIKQIKI"
     gene            complement(17173..18285)
                     /gene="vorB"
                     /locus_tag="PL000_06670"
     CDS             complement(17173..18285)
                     /gene="vorB"
                     /locus_tag="PL000_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNC88616.1"
                     /translation="MPKELWKGNEAIAEAAVRAGLEAYFGYPITPQTEILEYLSRRMP
                     ELGRAFVQAESELGAINMVYGAACTGVRVMSSSSSPGVSLMMEGISYIAGTEVPAVLV
                     NVMRGGPGLGNIAPAQADYNQAVHGGGHGDYQPIVLAPASVQEAIDLMVLSFDLAEKY
                     RSVCMVILDGSVGQMMEPAQMPEMRPVQRANWDWATDGRMGKRERRVLSSIYIEPAEE
                     EKMNLRLLNRWRTIQANEVRYKEYFLDDAEIVIIGFGTAGRVALSAVREARAQGIKVG
                     LLRPVTVSPFPSAVIEQLAGKVDAFLVTEMNSGQMLEDVRLAVKGRVPVEFYGRLGGV
                     VPFPDEILNEIRRMAKSKMAVNANPRDAWLERMAAG"
     gene            complement(18366..18617)
                     /locus_tag="PL000_06675"
     CDS             complement(18366..18617)
                     /locus_tag="PL000_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC88617.1"
                     /translation="MAAKGWIEVSDTYCKGCEVCISACPQGVMEINMSHLTPKGYHPA
                     HTFKDGCTGCAICALVCPDAAITVYREITKNAAPVTVTA"
     gene            complement(18741..19364)
                     /locus_tag="PL000_06680"
     CDS             complement(18741..19364)
                     /locus_tag="PL000_06680"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88618.1"
                     /translation="MDCPPNLIPAPGQYLLAHASGSTSPLPVSVYFYDSAPKGFRFAP
                     PLPPTWTIGTQLNLRGPLGHGFSVPANTRKLALIALESSSARLNGLIPIALKQGAEVV
                     LVCDSAPSGLPEVVEVQPLRSLEEICKWADFVAVDSARETLPQVKKLFEKLNQVSAVR
                     EAQVLIQAPLPCGGLAECGVCAVSSHHPHEWKMACKDGPVFEMNFFI"
     gene            19459..20475
                     /locus_tag="PL000_06685"
     CDS             19459..20475
                     /locus_tag="PL000_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNC88619.1"
                     /translation="MNIAGVIQAVGSFAWLVFVGLLVMIGVRSSRNQPSKGFGTGVVA
                     SLVVAILLTTLGAGIVFIQPEERGIVISAFSSKGYREQSLQPGLRWIIPFAEQVTLYP
                     ISRQTYTMSSSPTEGQIVGDDSIRARTNDGQEILIDASVIYSIDPSKIIELHINWQSR
                     YQDELVRPASRNTIRDAVSQYSVEEIVSTKRAEMEEEITRSLTEAFKENDLVLVSFLL
                     RDIHFSEEYAAAVEQKQIAEQQAQQAKLVVEQKKQEAEQARQVAQGQADAAVIAAQGA
                     AEARLIQAQAEAQANQLLAASLTPTLLQYQYILKLAPGVQTIFIPSGNQFILPLPDTT
                     LQTQ"
     gene            20616..21674
                     /gene="selD"
                     /locus_tag="PL000_06690"
     CDS             20616..21674
                     /gene="selD"
                     /locus_tag="PL000_06690"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20676..20678,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNC88620.1"
                     /translation="MSIPQSGREIRLTTLSACAGUASKLSADALTQVLRPIKNVFDPA
                     AYPDLLVGLASPDDAAVWRLSEDKAIVVTTDFFTPVVDTPYEYGSIAAANSLSDVYAM
                     GGQPFLALNVAALPDHLPIEISSEIMRGGAEKAREAGVVIAGGHTVKDKEPKFGLVVI
                     GFVDPRKMISKGGLRVGDVLVLTKPLGGGVTTTALKQEKAEERHIAEAIEWMSRLNKT
                     AGQLALEFDLRGGTDITGFGFLGHGLEMAEASGVRLSIENSKVPLLSGARGYAEKGTF
                     PGGAFDNKGHVGENVRFDVGVDEPAQMLLFDPQTSGGLLLGVPREKLSAFETRARELG
                     QPVWVVGEVLDGQGITVQ"
     gene            22019..23314
                     /locus_tag="PL000_06695"
     CDS             22019..23314
                     /locus_tag="PL000_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC88621.1"
                     /translation="MSIINEIVLILILILINALLAMSEAALVASRKVRLQQQANDGNK
                     SSELALKLLKDPNIFLSTVQIGITLIGVLAGAVGGATISEALALALQKIPYLGEFSES
                     ISLAIVVLTITILTIWLGELVPKRLGLTSPEKIAQVVAGPMLFTSKAFSPLIKLMSKA
                     TNFALSLMGVDPSSEPPVTEEEIHLLIDQGTQAGVFEEAEQDMVEGIFSLGDSRVYSL
                     MTPRTEVVWLDINDSIEDIREKIAECPYSRFPVRQDSLETIVGIVKSRDLLVESLSGK
                     AVDLKSLLKPAHFIPETMFASRALELFKEKNTELLLVVDEFGGLQGLLTINDILEEIV
                     GAMEFEEPQATQRQDGSWLLDGMLEVDEFKEIFDFESLPHEDEYETLSGFVMTSLGRV
                     PQAADHFEWNNFRFEVIDMDGRRVDKVLVTTLPKPQPPQ"
     gene            23441..23728
                     /locus_tag="PL000_06700"
     CDS             23441..23728
                     /locus_tag="PL000_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNC88622.1"
                     /translation="MTAYVIVDIEVSDPEGYKEYVKLAPEAVKLYGGKYIARGGYNET
                     LEGDWQARRLVILEFPSVEQAKNWVNSPEYAPARALRHKYARSNMVVVEGV"
     gene            23755..24924
                     /locus_tag="PL000_06705"
     CDS             23755..24924
                     /locus_tag="PL000_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC88623.1"
                     /translation="MSQESNPNQKTKVCPTCGTRLSENAMRCLVCGTEFNAKPEPKAA
                     KKVEKSVQASRMPVITMSLPAAVGALAVVVIVAAAIVFFSLRASAPPGTFETAETATP
                     SATPTETQPATATLIPTQVPTSTNIPPFDYTVSAGDGTCSQIAFNFNISVQSIIIANN
                     LPSTCPISVGQVLKIPYPTPTVPPAATNTPEAAEATRISCETVPITVQDGDTLSTIAL
                     NYAVPPEAIKEFNGLPTDNVFIGSSLLIPLCKRAATPGPTPTATIPPPYPAPNLLLPA
                     DGAAFTLANDVVTLQWASVGVLRDGEAYQITIEDVTSDQTRRLTDYVTDTKYIVPTSF
                     RPKDNVAHVLRWWVTPVRQAGTDEQGQPIWVASGAVSDKHVFTWVGVAVEGTPNP"
     gene            25084..26184
                     /locus_tag="PL000_06710"
     CDS             25084..26184
                     /locus_tag="PL000_06710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC88624.1"
                     /translation="MSPFELNLEKTRTETSLVGLEPALNAFGSMLLISKAEDEPGIHE
                     WVSKTRGEMSAEEKFRHGLVTIGFYYSILPQKGGISFEAYLDGLEATPPSVFRERLLK
                     AYEMICITEEAKANQGESVNWEETLSSAENYVDFLKYRFGEDLTNVEMETRAYQYVID
                     PAALKQLVTGHIRWFWKNHLQAEWARVRPMLEESARAFNKLDTDDMTRLEQLRFVTGK
                     DAQETKWADTLENADRVTFVPNAHIGPYMHTTRVGNDLYIYFGAHLPEGSDVQIPELD
                     RAEIVSKLSALADETRLQILQMIAENGKMRSNEVMDATRLSQPSVSRYLGQLTASGFL
                     QERRTGGAKEYSLNRERIEKTLKAVSAFLLDR"
     gene            26205..26444
                     /locus_tag="PL000_06715"
     CDS             26205..26444
                     /locus_tag="PL000_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88625.1"
                     /translation="MNPLLEEYLVEEHQREIEREMNSAQLLGQTVKAGVYRPTLSTRL
                     LRGFGQWMIEKGEDLVKRHEIPAQNRRTADQRYAH"
     gene            26530..27768
                     /locus_tag="PL000_06720"
     CDS             26530..27768
                     /locus_tag="PL000_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88626.1"
                     /translation="MLARLKSIYHEYPKAFWVYNIIVFIDRFGGFMLYPFFALYLTKK
                     FEIGMATVGILFGIFSISGLVGSALGGAITDRMGRKGVIIFSLLLSALSALGMGYAPT
                     LGIFVAVSVIVGTLSSIGGPAHEAVVADLLPENKRAEGYGIIRVIFNMAVIIAPATAG
                     LLIAKSYILLFIIDAVISVVAAGIVLFALPETKPKAHPDAKHETIAQTFAGYGKVFKD
                     IPFMGFVFVTILMALVYMNMNTTLGVYLRDSHGLPELNYGYLLSMNALIVVFFQFWVA
                     RKLEKYKPMLMMALGTALYAIGFAMYGFTSTYLLFVVAMIIITIGEMVVSPFQQALVA
                     SFAPEEMRGRYMAISGLSWGIAFAAGPYMAGWVMENINPNWLWVACGVIGGVAMLGYI
                     VLDRIHHSPVSMAVEPAAAD"
     gene            complement(27833..28750)
                     /locus_tag="PL000_06725"
     CDS             complement(27833..28750)
                     /locus_tag="PL000_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88627.1"
                     /translation="MRKYLFITLLTLFALSCGTPAAAAGESLTAADLPVETVTQPAVK
                     NESPKSATTSTTMLYVSATMHIESQPDSWPKDVDAFLAFIQQLTDAGVRLSIGADVGW
                     LTGGIRVQEIIQRTSAMGVQWDVHAHKQSDRAKAAYLLAKYGATPTSVISGMQISEFD
                     KARKPSSYKGYTWTPKVVWGGVACIGHRPGCDDTSIVLYRPTSSAKYTVHDPKGNIVR
                     VGNTDHQLATAESLLAQIEAGAYSVPMLSFTIMIEPETLLIVNSDDGLTEILAFVEQV
                     KENPNLRFATIEETAQAWVAAGSVPIQIP"
     gene            28848..29621
                     /locus_tag="PL000_06730"
     CDS             28848..29621
                     /locus_tag="PL000_06730"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC88628.1"
                     /translation="MDANHDVRSYNRAAWDNQVRSGKNPWTVPVSPEVIANARKGDWS
                     VLLTQNIPVPKKWFPPMQGLKILGLASGGGQQGPVFAAAGAEVTIFDNSPAQLERDRE
                     VADRDGLNIRTVEGDMRDLSVFADESFDLVFHPVSNLFIHEIRPVWKEAFRVLRKGGT
                     LLAGFMNPLFYLFDWEDMDNGILNVRHKIPFSDIETYGMERLKQEDRPAEFGHSLTDQ
                     LTGQMEAGFHLIDMYEDHHTGLRISEFTPTYIATRALKP"
     gene            29630..30451
                     /locus_tag="PL000_06735"
     CDS             29630..30451
                     /locus_tag="PL000_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNC88629.1"
                     /translation="MKILIAADMEGITGVVHWDQVNPKHPEYARFRKLMTADVNAAIR
                     GAFAGGATSVVVTDGHNSGRNILIEELDARAMLNAGSPAPLSMVQGVDDGVDGVMFIG
                     YHGRTGAADAILDHTWSESVSGVWVNDRAFGEVGLNGAVCGHFDVPIIMASGDQTLCA
                     EVREFFGSEVETAQVKKAVGRMAAECLTPAASASLIEEAAKRAVINLRSGRKTKPFKV
                     SKPIKMTLEFAQSEMADKAALMPGAVRTVDRRVEFSAEDMVTVYLAFRVLMTLAG"
     gene            30515..30697
                     /locus_tag="PL000_06740"
     CDS             30515..30697
                     /locus_tag="PL000_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88630.1"
                     /translation="MAKNVVKGLAVLFFLAGGTWVLQGINVLPGSFMTGDPQWAINGG
                     ITMAVSAGVFWFASRK"
     gene            30723..31142
                     /locus_tag="PL000_06745"
     CDS             30723..31142
                     /locus_tag="PL000_06745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HNC88631.1"
                     /translation="MSDSKVKLERKAKKMIKNEYVIWLTTVDAKHVPQPRPVWFIWDE
                     DSFLIFSQPKAHKVQHIRENGSVSLHFNTDEKGDQDVVIYIGMATLDAKTSPAHKVPA
                     YLRKYRKGIKELGMTLEQFSSEYSTAIRVKPTSLRGW"
     gene            31152..31379
                     /locus_tag="PL000_06750"
     CDS             31152..31379
                     /locus_tag="PL000_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88632.1"
                     /translation="MAESIQIGDRVKIYLDSKFGEKEGWYNGTVFKVDPYSAHRSFYW
                     VELTTEAQTLLGIRQISVFNLKNIQKLQPDS"
     gene            complement(31471..32268)
                     /gene="arsM"
                     /locus_tag="PL000_06755"
     CDS             complement(31471..32268)
                     /gene="arsM"
                     /locus_tag="PL000_06755"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNC88633.1"
                     /translation="MTQSPTPIHETVREHYAERIKSSASCCGPSDCCTPNNILYPEQL
                     LTTVPSDVASTSYGCGDPITLASLKPGQTVLDLGSGAGLDCILAAQKVGESGHVIGVD
                     MTPEMIERATANAKKVHLTNVEFRQGYLEDLPVESNTVDVIISNCVINLSPDKEKVFT
                     EAFRVLAPGGKLAVSDIVTAGILPEPVRQSLSAWAGCVAGAVQAEEYIAMMNSVGFTD
                     ISITPVFFDKEFVESAIAEMNLDVSNYPREEIYKAIFSAKITAYKPA"
     gene            complement(32289..33254)
                     /locus_tag="PL000_06760"
     CDS             complement(32289..33254)
                     /locus_tag="PL000_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNC88634.1"
                     /translation="MDFPSLLQYTFGKVVETFSHNWFLLLLSIIASAALKLYVDEDAV
                     ARFLRRNTKNSVLMSTGVAVATPLCSCGTTAIVLGMMASTIPWAPIVAFLVASPLTSP
                     QELFYSAGLFGWDFAIAFFVASIVLGLAGGAIASFAESRGWLANQARMAAPKFAATSM
                     GIMGGPVPLDLPVIMQPVAKTEKPTVTFQMFIQEMWTVSKRLLPLFLGFTFIGYMLNG
                     MIPAAWITSLFGSGHVYSIPLAATLGLPFYINTEASLPLVRAMIDSGMSQGAALAFLI
                     TGAGTSLGALGGALTIARWRVIGIVVGTLWVGSIILGFAYNLFIM"
     gene            complement(33512..33823)
                     /locus_tag="PL000_06765"
     CDS             complement(33512..33823)
                     /locus_tag="PL000_06765"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC88635.1"
                     /translation="MKFNPVLFAKAISDETRQKIMNICCCSSLSVSEIVEKLDVSQPT
                     VSHHLAILREADLVTVREDGKQTFYSLNQDNVAYCCGQLMVKFAPESVATENLIKTIK
                     A"
     gene            complement(33977..35701)
                     /locus_tag="PL000_06770"
     CDS             complement(33977..35701)
                     /locus_tag="PL000_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88636.1"
                     /translation="MRKNPLFLLLSLFVILSLFLASCGGEEGEYYTEEATNDEYLTEE
                     PFATEAPASESEGGAPAATNLPGEVVYDLGFNPVDNGFSFSNYGDDIPATNLTADEVR
                     RMFGDQVCARLDGDTCTLTPPAEQWMEQINGAMAGGHCEGMAVLSLMMYTDQVSPSNF
                     GGSTASELDINDEALQREIAYWWATQAVAPTNTSIIKGTPVEILEIVQQMDAKGETYT
                     IGIYKEDGSGGHAITPIGVEDKGNGLYAILVYDNNYPGETRELYVDTRDNTWLYEASI
                     NPQVQSELYTGNADTQTLDLTPTSARLNTQQCPFCNGSGVSSFTGGKLTSPSLQGSQI
                     NQIFLDGEGHILITDENGNRLGYVDGKIVNEIPGATYSAYRMEASGNAPEPIYSLPAD
                     LDVTVSIDGSALTEETLTDLIMIGAGFSIGVEGIYLEPGQVDTAYFYPSEETIAYETE
                     ADESPFIVIGVENPDAEADYYFEVQGADIQGGGIITVYLDTKAGDLLINAEKLNNEGF
                     FDFYLTRITDELEEEFAAEEIALQEGAIVYVNYAEWNDANPEGMYFGVDLDGDGTIDD
                     EYVVDDSQ"
     gene            complement(35783..36625)
                     /locus_tag="PL000_06775"
     CDS             complement(35783..36625)
                     /locus_tag="PL000_06775"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88637.1"
                     /translation="MERLRFLHIPKTAGTTFTTCLDYQYSGKGHFVFKGAVQYDVMRF
                     KELPEAEKQKVVLFTGHAPILTGLEEADTAKIITFLRNPISRVKSFIQHISEGKSPEY
                     LKGPFNLDEFLASGTKDLENLQTKMLINKGPFALSTLIDSMSKSEARDLALNNLFNKV
                     EEYGLQEYFDESLVLFASALNWSTPFYTSLNKKDSSRLLEFKQHHLDRIAELNSIDIE
                     VYNTAKARFLQTLESPKFDKIKLEKLRYKNSLASPHKHAWNLWYSFYRLKRRVRRYLG
                     DESS"
     gene            36908..>38054
                     /locus_tag="PL000_06780"
     CDS             36908..>38054
                     /locus_tag="PL000_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC88638.1"
                     /translation="MKNISFFRSICLRVFLSIIFLTSINAVRMNDGPQSPAQVDEAAA
                     TIGTCPTFPQDNFWNTPVTGLPVHPMSDAWVSAIGTNTGFHMDFGSGTWDGGPIGIPY
                     NIVSGSATTKYTVDFYYPDESDPGPYPIPNSPNREWGSDHHILIVDTDDCTLYELYDA
                     SQTTNGWAAGSGAIWDLNSNALRPDGWTSADAAGLPILPGLARYEEVATGIIEHALRF
                     TTNCTADYYIWPARHVAQSGSCANPPPFGARFRLKADYDISGFSPQAQVILQAMKTYG
                     IVLADNGSPWYVSGAPNEGWDNDVLHEMDVLTGASFEAVDTSGMMTTTTFADVPESHW
                     AYQYIEQLYVAGITGGCSTNPLMYCPDSTVTRAQMAIFILRGMHGSAY"
BASE COUNT         8872 a         9345 c        10252 g         9485 t
ORIGIN      
        1 tttttttcaa tcttgtccat ctcttggggc attttatttt cagacaagcg tggtaagatt
       61 cgtccaatga aactcaacct tgccctcgcc cagatcaaca cccggcttgg agatgtggag
      121 gcaaaccttg ccaagcatct cgagtacatc gaacaggcca aagctgaaaa gaccgattta
      181 ttggtattcc ccgaactttc gttaactgga tatgtattgc aggacctggt cgcttcggcc
      241 gcacacaagc cgacagagga tgacccgatc ttcaaacatc tcctcaaagc gtctcacgat
      301 ctggacctgg tcattggatt cgtagacgaa gactcccgtc accgctttta cattgcctcg
      361 gcataccttt caggtggcaa agccattcat gtccatcaca aagtctacct acctacctac
      421 ggcctttttg atgaaggccg cttttttgcg tggggtgact cggtccgtgc gtttaacaca
      481 cgttttgggc gcatgggcat gctcatctgc gaggacttct ggcatgcctc gcctccgtac
      541 ctgctttggc ttgatggtgc agatatcatg ctgttctcgt ctgcttcacc ggggcgtggc
      601 ttgaacgaca aggagaaact cgaatctgcc cgctgggtgg aacgtgttac caaggcctat
      661 gccagcatgt tcacttcctt tgtcgcccat gccaaccgtg tcggctatga agatgggctt
      721 catttctggg gcggagccac cgcttttgac ccgaatggcg aagaacttgc tcacggcccc
      781 tacaatgaag aagccatcac ctacacccag ttggatctga atcaactccg ccgtacgcgt
      841 gcgcgccttc cgcttttacg cgatgaacgc accgccctca ttcgaaacga attggacagg
      901 attctctccc gtgactgacc tcaacctcac catcaatgca gaccttgcgc gtgaaatcct
      961 tactggtttc atcaaatctg agatcacgcg agtgggactc actcgtgcag tggttggtct
     1021 ttcaggtggt gtcgactcgg cgctttcgtg tgcattggct gccgaagccc ttggcccgca
     1081 gaatgtcctt gccgtgcgca tgccctacaa agcctccagt aaagattcgc tggagcatgc
     1141 ccaacttctc attgaccagc tcggtgtgca gagcaagacc atcgagatca ccgacatggt
     1201 tgaaccgctc ttcaaacttc atccagatat ttcgaacatg cgcaaaggca acatcatggc
     1261 ccgcgaacgc atgatcgtgc tgtatgacca gagcgaagta ttcaaaggat tggtggttgg
     1321 caccagcaac aaaactgaaa ttctcctggg atacagcacc ctgtttggcg attctgccaa
     1381 tgccctcaac cccatcggtg acttgttcaa aacccaggtc cgccagcttt cacgggctat
     1441 gaacatccct gctcccatcc ttgaaaaacc cccgtcagcc gacctgtggg cagaccagac
     1501 cgatgaaaaa gaactcgggt tcacctatga agaagccgat aaacttctct acctgcttgt
     1561 agaccaacgc tacagtccgc aggaagccat cgaagtgggc ttcgccgaag atttcgtcaa
     1621 aaccgtcacc acacgcattc gccgcaatca attcaaacga atgcagccgc cgatcgccaa
     1681 agtcagcaac cgcaccattg gctacgactt tttatatctc cgcgattggg gaacataaat
     1741 gaacaggagc cagtgtgcag tgatacctga aaactgttca ctgccttctg tgctgatcac
     1801 tggaaataca aatgcatatc ccgccttctc ttaaacacaa aaaatttttc tacctctggc
     1861 tcggacaact gatctcgatc acaggaaccc agatgcaact ctgggcttta ttttggcatg
     1921 tcagcaatct ggataaaaac ccgatcgccc ttggcggaat cggccttgca cgcattctcc
     1981 ctgtgatcgt tttttcgctg atcggcggcg cactggccga ttcagtggac aggcgcaaag
     2041 tgatgttcgt gacccaaagt gctgcggcgc ttctcgccct gaccctcggc ctgctcaccc
     2101 aatttgggca ggtcaccatc tggtacatct acgtcatcac cgccattcaa gccattgcca
     2161 tcgccttcga tggcccctca cgtcaggctc tgattccaaa cctcgtgccg aaagaggatc
     2221 tgcccaacgc tttcagcatg accttcacct cattccagtt tggctctgtg cttggacccg
     2281 ccttgagcgg gtttgtcatt gcctgggctg ggcaggaggc ggtttactat ttcaatgcga
     2341 tctcgttcgg cgcagtgctg ttcgccctgt ttatgatcgg agatgttccg caatcgtttg
     2401 ccgagaaatc ggcaggcatc agcatggatg cgattcggga aggcattcgg ttcattcttg
     2461 gcaaacccat catcctctct tcgatgctgc tggattttgt ggcaacgttc tttgcttccg
     2521 cgaatacgct gatgccgatc attgccacga ccgttttgaa ggtgggggtg gtggaatatg
     2581 gctatctctc tgcggcttca gcggtgggtg ctgtgacggt ggcgctggcc atctcacagg
     2641 tcaaggtgat tcgcaggcag ggtcccatgt tcatgggtgc tgtcatcgtg tttgggattg
     2701 ccacggtcat tttcggcctg accagatctt tctgggtggc gtggctggcc attgccacca
     2761 cgggcgcagc ggatggagtc agcacgatca tccgcaatac gattcgacaa ctgcaaacgc
     2821 ccgatcacat ccgcggacgc atgaccagca tcaaccagat attttttcag ggtgggccgc
     2881 agcttggtga aatggaagca ggttcggtgg cgcagttttt cggcgcatcg tttgcggtca
     2941 tcagcggcgg cattggctgt atcatcggca cactgctgat catgtggaag tggccgcagt
     3001 tgatgcatta cgacggccat gagccaatcg aaggcgagat cgccccatcg ccttcttcag
     3061 gggcatccat tgttagcaac agggtgcggc ggccgtaata aaaaaagcga cacggcagcg
     3121 ccgtgtcgct acgatgattc gatcaaattc ttcatctctg ccagaacggc ttgatgaatg
     3181 gctgcagcat catttgtccc caatgctttg acataaatcg gatttccgat ctgcacagtc
     3241 actatgactg gcttttcatt gaacatgacc atgcccagca attgcagggc ggtggcaagt
     3301 ttttcttttt cttcttgtgt ctttttgatt ttcaacagcc agtgatcggc gtattttgca
     3361 gagaccacgc cgcgaaccag cacgggcagg atcgccgctt caggagccat gcgcacgaaa
     3421 acgccgacac tatctgtcca tgcttgcagg gagtcaactg cgcctgggta tacctcgggg
     3481 tcgggttcga tgtgacctgc agggaaggtg agcgccgcgc cgccgttctt caaatgggaa
     3541 ctgacctgac gcacaagggt catacgcttg gcggggtcat cgtggacgta gaatagctgt
     3601 ctggttgtgt gggggagcgc attcagaaaa gggcgatcaa gggcgatgat atgcaggtct
     3661 ttgcgattta aggcggcaaa gagtgcgacc gtgtcggtca tgcctggatg attggataat
     3721 gccagaaacg ctgaatcagg cacacggtcc gcgtgaagga cgcgcaattc ttttacatag
     3781 cgattcaaca ggttgcgtcc gccttcggca atgccgtgca tgccggtggt gtcgtcatat
     3841 tccatcatgt gacgggcaaa ggtttcggca ggtttgcgga aaaggaatcg caaaattttg
     3901 gcggcaaggg gatgattttg ccagccaaaa gaggagacga ggtcgtcgag gttgatctgt
     3961 gtgagtgttt cgaactggtc caatttattt ctttgcggtg gcccacatgt tgagcatgaa
     4021 ttcaaaatag ccgaattttg ttttggtcat ttcaaaacca gcctggccga aataccagcc
     4081 cagcagcggc agggtgtagg cgtaggcatg ttcatggatc tcttcggcgc tgacaaggcc
     4141 gatccgcgcc atgaatttca gcagtccgtc ggaccatgct gcgggggtgg tgaggatgac
     4201 catgccttcg ggttttagca cgcggtagat ctctttgaaa agttttgcca tcaaggcggg
     4261 gtcgaggtgt tcaacgacgg ccagcaaagt gaccgattcg aaataatcat tttcaaatgg
     4321 caggcgcggt tcgatctcga gatcaagggt gaaactttcg atcttcagtt cggaggctgt
     4381 ttgttggggg agcgggatct ggtcgatcgc aaatttttca gcaaaggccg tgtgtgccaa
     4441 aaaatatgga aaagagccgc aaccgatatc gaggatacgg ccgttgcgta gatttgaagg
     4501 gatgagcttg ttcgctcgtt gtgcgcgcag gtctgcgagc atgggttcga gcagaccttt
     4561 tccgcgggtc atacctgcca tgaaaatgtt ctccaatttt tgaattatcg ctttttgcgt
     4621 aaattacatt tggggcataa ttagtcgtct gaaagagttt acaaaacttt tctcgctcaa
     4681 gccgccgtcc tcggcggcgg ggacgccgcc gagagcatgt ttttatgaaa tttatttcgg
     4741 cagactacct gatctttgcc tggtagatta tgcgatacca tgaaattcgt gcaactatat
     4801 cccacattta ttttccatgc aatctacgga tttgttgaga ggcggggacc cagcgattcg
     4861 ctgcaatgaa agcccgttat tctggttcca tcgaaaaatg gagcggatat ccctccaacc
     4921 ctgcggcgaa gtgggctttg ttgatgcgcc gctccgcctc agatttggcc acggtctgca
     4981 cgtaggccat gcctgtgatg tgggccttga gcatgatctc ggcggcggtg ggtgtgccga
     5041 gataaaagaa ggtcgtcaac gcatgcacga cgaaatccat cggggtcaca tcatcgttgt
     5101 ggatgaggat gcggtagagg ggctcgagtt ccgtttcggt gatctcgatg atctcaattt
     5161 cgggggcaat ttggttgttc atgaaaagga tgaatttttt atatataaag gcggtttagt
     5221 tgttccacct cttgtgaggt gaggacgatc tcggctgctt cgaaattttc caggatgtgc
     5281 tgcgggttga acgacatggg aatcgtgatg acgcgcggtt gggagaccag ccatgccagc
     5341 gcgatctgat aggctgtggc ggaatgtgct tcggcgatct cctgcagggt tttgttcaca
     5401 ggcaggttgc ggaatttcac gggtgaatat gccgtgatca gaatatcgtt tttctggcaa
     5461 tattccacca cgccattttc cacgtaggca cggtctggca ggcggtaggg tacctgattg
     5521 gtaagcaggg gtgtttcaga aagttcctgc gacttcttga ggtctctgag tttgaagttg
     5581 ctcactccca aatggcgcac tttcccgtcg cggacaagtt tgttcagcgc ggtgaaggtc
     5641 tcttccagtt tcatgcccgt tgaaggccag tggatgagat acagatcgac atattccatt
     5701 tgcaggcggc gcaggctttt ttcacaggat gaaaaaacat ccttggggtt gaggtgttcg
     5761 ggtgaaacct tggtggtgat gaacaggtct tcacgtttgg tctttgtctc gcgcaccaca
     5821 cgcccgatca attcttcagc gtgcccgctc gaatacattt ccgcggtgtc gaagtgggta
     5881 tagccaacct ccagggcaga gcgcagagcg gccatcgaaa cagaatcaag actcgggtcg
     5941 ggggttgatt cgccgccgat cttccaggtg ccgaatccga tcttggggag ggtcaggttt
     6001 gggagggttt caaatttcat cggtgtatta atccaattca atttcgatcg agcgtgtcac
     6061 tttggccctg ccgccagcga gcaggctttt gatgctgcca tcttccagtt tcaattcgac
     6121 cagaatttcg ctgggtgatg gcgttggcat gagcctgcct tgttcgagaa tcagcgtttc
     6181 tttgttgatg tttatttggt cgaataaata acatgccagc ggtcccgccg ccatgcctgt
     6241 ggccgcttct tcgggaatgc cgtaatacgg tgcaaacatg cgtgttgcgg catcgcgccc
     6301 gcggacttgt gtttcgagcg tgaagggata atagccgatt aaatccaacg cttcgctgat
     6361 ctggtgaatg gcgttcaggt cggggtggat cgctttgagc gtggcttcat ccttcacggg
     6421 aatcatcatg aacgaattgc ctgtgttgac gggcatgggg ttatgaccag ccagcaggtc
     6481 ggtcttttgc aggtgcagtg aggcgagaac atcctgttcg ctgatgcctt tgtaggggag
     6541 cggaaaatac tttggcgcaa gttgttgcat gtacgccact tcacccttta aataaatatc
     6601 gcgatttcca tcgatggttt cttttgagct tttggggcct ttcacaattc cctgctgcga
     6661 caaataggag aacgtggcga tggtggcgtg tccgcagtga gcgatctgtt tgttgggcgt
     6721 gaagaaatcc agcttgaatt ccgcgctttg tgatctggaa acaaatgccg tttcagaaag
     6781 cccgaccttt gcggcgatct tttgtttgtc ctctttcgta aattcatcgg cagggtaaac
     6841 aacgccagcg ggattgccgc cctctcccgc gtctgtgaat gcattgataa tgttgatggt
     6901 gatcttgttg gtcatgggtc ctctgtaatt gtatggctat tgtagcatcc atttctctcc
     6961 cgatattctg cacaaattct gcacatctaa aaagtatcct ttggaaaatt tgaaggagaa
     7021 caatcatgca aaatatgcta aagaacaaaa gaaattggtg gattgccgcc atcctcgtgg
     7081 ttgctggtgt gttggtttgg gcgggtgtgg ccgccacagg ttctgcaaat gcggcagaag
     7141 cacaaacaga acaggtcatt gcgatggaac tggcacaaac tgttgaaact tcaggcacac
     7201 ttgaatccca gccctttgcc agcctcaatt ggaagacagg cggaattgtt gaatcggtcc
     7261 atgtgcagcc tggggatttt gtaaaagcag gcgatgtcct gttgactttg cagcctgaga
     7321 gcacatccgc ttctctggcc tcggctcaag cggacctgga aactgctcaa gccaacctgc
     7381 gggcattgac cttccctgat ggttcatccg ttggggaagc ccgtgagaat gcatccagcg
     7441 cgttcgacgc gtggagcgat gcacgggtgg atctgatgga tgcacttgcc tacaatcgcg
     7501 gcggcggcga tgacgatttg tacagcgatg tggtgactgc ccgcgacgac cttgtcaatg
     7561 cgctggagga atatccgctg gcggcgaatg tggatgcaca attttattac tgggcggccc
     7621 gtgccgatgc actgggatat cacggtgagt atgatcatgc cgcccttgta cctgttttcc
     7681 gcgctgagtt gaacgccgac gatgctgaac ttgtcgatga gatcctcaca gcccagaccg
     7741 aatttgagac tttggccaaa gccttcgccg aatcaatgga agatcaggat gatgcggtcg
     7801 acgcgatgaa agcctttggc gcctacgaac aatcggctga tgccttgttg gatgctatgg
     7861 aagcggaata cggtgtgctg gttcagccca gcgaaagtga cctggcttca gctcaggcaa
     7921 aagtggacgc cgctcaggcc agcgtaaata atttgcagat cgttgctccc ttcgatgggc
     7981 aggtgctttc agtggatgac ctggtgggcg ataccgtgag tgctggcgat ctttcggtga
     8041 acatggctga tatgaataac ctgtacgtgg aggcccaggt ggatgaatcg gatgtggcca
     8101 atgtcagggt tgggaatcag gttgaggtca cgttggatgc gctgcctgac ctcgtgttga
     8161 caggttctgt gacggtggtc aatcctgtgg gtgaagtcgt gggcggtttg gtaaagtatt
     8221 ccatccgtgt ggagcttgac cccgtgaaag atgatttcct ccccctgggc gcaacggcaa
     8281 atgtggttat caaggtcagt gatacacagg cttctctggc agtgccgatc atggcaattc
     8341 aaaatgacaa tgaaggcgag tatgtgtggg tgctgcgaga tggtgaagcg gtgcgtgtga
     8401 atgtgacagg cggcgctctg gtagatgggt atgtggtggt aactggtgac ctgcaggaag
     8461 gtgagtcttt gcagcttgtg gaacgcgaaa gcggctttac tccgcccaat ccattttctg
     8521 gtggtgcgaa atgattcaag ccgatggttt gaccaaggtt taccaaatgg gtgacagtga
     8581 agtgcgcgcc cttgatgggg cgagtttcac catcaaaaaa ggcgagatgg tggcgatcat
     8641 ggggccttct gggtcgggca aaagcacgtt gatgtcgatc attggctgtc tggatgtgcc
     8701 cagcgaaggg agttactcgc tcgatggtga agccgtgcag acattggatg aaacaaagct
     8761 tgccgatgtg cgcggacgga agatcgggtt tgtgtttcaa cagtttaatc tgctggcacg
     8821 gacgagcgcc ctggaaaatg tgatgctgcc actgacctat gcgggcattt ctggcaaaga
     8881 acgcgaagac cgtgctaaaa aggcgctgga acgtgttggg cttggcgggc ggaccagtca
     8941 cgcgccgaac gaactctcag gcgggcaaca gcagcgtgtg gcaattgcac gagccattgt
     9001 caacgagccg agtattttgc ttgcggatga accgacaggc gcgttggata gtaaaactgg
     9061 cgtggagatc atggacttgt tccaacgcct gcacagggaa aatggtcaga cggtgatttt
     9121 ggtgacacat gactcgtacg tagcccgtca taccgagcgg atcatcaaaa tctccgatgg
     9181 aaggatcgtt tcagatgaag tgaacccgaa tcccatcaag gcgggtacgg gcaggggaga
     9241 ggagtaacca tgttgtattc agaaaatctc aagatcgccc tgcgtgccct gcgcgcgaac
     9301 aagatgcgtt ctgtgctgac cattttgggt attgtcatcg gcgtggctac ggtggtggca
     9361 ctgctttcga tcggcaacgg cgcaaccgcc agcatcaccg atcagattca aagcggcggc
     9421 tccaacctgc tgaccctttc tccaggcagg atgcaaactc ccatgtccag tttggggcag
     9481 tcacaacagg cgagctactt gtattattca gattatgaaa cgctctcaaa aacattaacg
     9541 ggtgagatca cctcgatcgt gcctgtttat caggcgagct acaccgtgaa attcggcgac
     9601 gaaagttttt caacctcggt ttcaggtgtg atggaagacc atgcgcaggc acgttcgttc
     9661 accatcagtg ctgggcgctt cgtaaccgat ggagataacg atgcactggc gcgggtggct
     9721 gtgctggggg ctactgtggc ggaagacctg ttcggcacac tttcgccgat tgggcagacg
     9781 atctctttga acggggtcaa gtttgaagtg gtggggctgc ttgagtcaga aggagcctcc
     9841 attggctccc ccgatgacat catctttgtg ccgctggaaa cgggttacat taaacttttc
     9901 ggtgatacgg caaaccacaa cagcaagaag accgtgaaca gcattttgat ctctgtcacc
     9961 gattcaaaag acatggatgc catctctgcc caggcggaat acattctgcg ccgcgcccat
    10021 gaattaaaac ccacggaaga gatcgatttc aatatcatga gtcaggctga tacgctcgaa
    10081 accttgaatt ctgtaaccca aaccctgacc gtcttcctcg gagcgattgc cgcaatttcc
    10141 ctgctggtgg gcggcattgg catcatgaac atcatgctgg tttcagtgac cgaacgcacc
    10201 aaggagattg gcctgcggaa ggcagtcggt gcaacgaaga atcagattct gacccagttc
    10261 ctcatcgaaa cggtcacgtt gagtttgttg ggaggtttgg tggggatttt gctcggggtg
    10321 ggcattgctc tggcgtttag cctctctggc ctgatcaact ccgtcatcac cgcagattcg
    10381 attttgctgg cctttttctt cgcgcttgcc attggcatct tttttggcct ctacccagcc
    10441 cagcgggccg catccctgca cccgatggtg gcattgaggt atgaataaac tcaaatcatt
    10501 ggtaatgtct ggaaagtgga aagtaggatg tcggtatact ttccactttc cactcccaaa
    10561 aaacacaccc tcttcacata gtctcgtata atcgtcccat gccaaaaaaa atcctcatcg
    10621 tggatgatga accccagatc gtggaaatct gccgcgatta cctcaaagcg gcaggttatg
    10681 aaaccgtcac agccagcaac ggttcacagg cgcttgcact ggcacgccgt gaaaagccag
    10741 atttgatcgt gcttgatttg atgatgccag aaatggacgg tctggacgtc acccgcgaaa
    10801 tacgccgtgc aagcgatgtg cccatcatca tgcttaccgc ccgcgttgag gaaaccgaca
    10861 aactcatcgg ccttgaactc ggcgcagatg attacatcac caaaccgttc agcccgcgtg
    10921 aattggtggc gcgcatccgt gttgttttgc gccgcgttgt aaattctccc gatggcgata
    10981 ccatccgtgc aggcagtgtg gagctggatc gtgcgcacta tgaagttcac ctgccagata
    11041 aaacggtttc gctcactgcc actgaatttg aaatcctcgc cacgctcatg agtcagcctg
    11101 gccgtatttt ttctcgtgcg cagctgctca ccgccactca tggcgtcact tttgaaagcc
    11161 tcgaacgtgc catcgattca catgtccgca acctgcgccg taaactggag cccgctgact
    11221 tgatcgtcac cgtgcatggc gttgggtata aatttgaggg ctaagatgca ttcatctgtc
    11281 cacgagattc gcacccgttt gttgtccctg ctggtcaggg cttttgtatt cgttctattt
    11341 ctggcgttga ttttctttct tgcagtagtg ggggctgttt tgacctcgca aacttcgccg
    11401 attcccattc cgttcgggcg ggcattggaa gggtattaca tcggccacgg caattgggat
    11461 ggggtcgaaa aggtttttga caccctcgaa gaattgaaga cccttgatgt gcttttactg
    11521 gatgaagggc aacgcatcgt tctggaccat gattctgatc ctgcatcggt ggtgggttcc
    11581 agctacgaaa taagacccaa cgatttcctg atcaatctga cggtggaggg acaggtggtg
    11641 gggcggcttg ccttcacttc gatcaccccc tggagcaggc tgggcattgc gcgtgcggtg
    11701 gtcattcctg tcggcattat ttcactgttg ctttccattt ttcttttgat cgttgccacg
    11761 gtattggttc gtcgtttcgt caatccgctg gcagatgtca tctatgctgc ccgtgaggtg
    11821 accaacggga aactcaccac ccgcatctcc agtgaaggtc cacaggacat gcgcagcctg
    11881 tctgaaagct tcaacgaaat gaccgccgcc cttgaacgca acgaccgtga acgccgtgac
    11941 ctgcttgcag atattgccca cgaactgcgc acaccgctct ctgtcattcg cggccgcctc
    12001 gaaggcattg tggatggaat ctacactgcc gatgatggac aagtatccct cgcccttgag
    12061 caaacctatc tcctcgaacg ccttgtggat gacctgcgtc tgttgaccct tgccgagacc
    12121 cgtcaattgc atttcgagaa aaagaaagtt gatttcgttc aactctcaca acacaccatt
    12181 gaaatgttct ctgccgaagc gcgggagaaa aatatttcga tttctcttga gatggggaag
    12241 gggaattaca cagcagaact tgatccgcag cgcatggaac aggttattgg taatttgatt
    12301 gggaactcac tgcgctatat cccagagggg agcaaaatat ggcttgcgct tgaggaaaca
    12361 tcaaaagagg tgaagctctt cgtcaatgat aacgggcctg gtgtggctca agatgaattg
    12421 ccctaccttt tcgatcgttt ctggcgcaaa gacaaatccc gcacacgcag tgcgggcggc
    12481 acgggtttgg gccttgccat tgcgaagcag cttgtcgaag cgcagggtgg gacgatccac
    12541 gccagcaatt tgcgtgaggg tggtttgcaa attgcgatcg aatttaggaa gatgtgatct
    12601 gctttttgtg gattgcccga tagatcggtt cgataaacct tgcaaggatc gggccaagaa
    12661 tggcaaggaa caaaacataa gcagcggaaa gcgcgccgag ttgaggctcc aagcctgcac
    12721 tgactcccag cccagcaatg acgattgaaa attctccgcg tgccaccaat gctgcacctg
    12781 cacgaaacgc ttccaacgga tttgatttgg cacggcgggc tgcccaccag cccgtcagga
    12841 tctttgtcag cgtggtgaaa aggcccagag aaacagccag cagcaaaacg ggaggtaaag
    12901 aagcggggtc gatctgaagt ccaaagaaaa ggaagaagat ggcggcaaat aagtcgcgta
    12961 atgggccaag tagtttgtga gtccgctcgg cgatggaatc ggagatggcg atgcccatca
    13021 aaaatgcacc gatggccgca gagacttgca ggtattgcgc catgcctgcc acgaagagca
    13081 ttaacccgaa tgtggtgagc aggatgactt cgtctgattg atgcgcgatg agcttgctga
    13141 attttttccc gtatttgaca gcgacgaata aaactgtgcc gacggttgca agtgcaatgg
    13201 aaacggagag aaccgcttgc gagatctcct gcccgatcag cagcacagcc acaatgggca
    13261 gatagaccgc catgatgagg tcttcgagca cgaggatgga aatgatggtg ggcgtttcgc
    13321 gattgttcat acgccccaac tcggataata cttttgcgat gacaccagaa gatgagatgt
    13381 aggtgacgcc gccaagcaat actgcggcaa gtggtgacca cccaagcacg aaaccaagaa
    13441 tgagaccagg cgggaagttc agcagaaaat ccacgatccc agctgggaat cctgaacgca
    13501 gattcgtgcc gagctcttca cctgaatact caagccccag catgaaaagc agaagaatga
    13561 caccgatctc tgcgccgata tgaacaaagt cttcgctgaa ttgaatgggg aggaggccgc
    13621 cattgccgaa ggcgaggcct gccagtaaat aaaaggggat ggctgaaagc cctaaacggc
    13681 tggcaaggcg tgtgagcagg gcaaggccaa tgatggctgc tccaagctca ataaaaacaa
    13741 gggagatgtc ttccatactt gattttacac aagaaagctc cccgtgttca attccatgtg
    13801 aattttctta tttagcttct gcaatgatcg cagtgacttc tatttcaacc aataatttag
    13861 gatcgatcaa ccctttcact tcgatcatgg atgcggcagg tttgaccgtt ctgaaaaatt
    13921 caccatgtgc cctgccgacc tcttcccatt ttgaaatgtc ggtcacgaac atgcgggttc
    13981 gcaccacatc ttgcatggat gcgccagctt caattaaagc tttttcgatc ttcgcgaggg
    14041 cgtatttggt ctgttcgtaa ggatcattca aaccgacgat ttcgcctcgc tcgtcgaccg
    14101 cagttgtccc tgccacttcg atcatattgc caaccctgac cgctcgcgaa tagccaacaa
    14161 tatcctccca ctttgcacct gaagagatgt tgattctttt gtccatggtg tcaactccat
    14221 tttgatacaa aataaaaaac cccgcttttc aggcgggatc tttgcctatt cctcttcatc
    14281 gtttttgaat tcgatgtcgt atttctcgcg tgctttttcc acctgtgcgt aatgttcctg
    14341 tgcccatttt ccaagtgcca ccacaggctc attgagcgat ttgccaagca gggtcaattc
    14401 gtattccacg cggggtggta cgacaggaaa tacatggcgt gtgacgatgc cattgcgttc
    14461 aagtgctcgc agggtttgag aaagcatttt ctgtgagatg cccccaactt cacgcaataa
    14521 ggcgttgtga cggttgattc cgcgtgcaat ggcgttcaat gtgagcagag tccatttgtc
    14581 tccgatcaat tccatcactt tgcgcgaagg gcaatattcg ttgaggatat ctattgggta
    14641 tgtgctcatg atacacacct tttggtaact accatacaaa ttagtgggct cttggaaggg
    14701 tttgacccgc cacttatact gattgtaaca gaacaggaaa agatgacaat gaacacatgg
    14761 cttactgcaa ttcacatcaa accagagcac gttgaaaaat ttctggcggc atcgactcct
    14821 tttgcgggag agagtctgcg cgagagcgga ttggtcagct ttgcgctttt acaacgagtg
    14881 gatgatattg cacagttttc gatgttcgat gtctatcatg atgctgctgc ccgcgagaat
    14941 cacctgacct cggcgcacta tctggcatgg ttgaccattg tcacaccgct tttggcggca
    15001 ccaatgacct ccctgccatt caacccgatc tttccgccgc ctgaagattg ggaacgccat
    15061 ctggaaacag aatcgtgatc ttcgattagg gttttcgtaa aaaatcaaat taaaaacagg
    15121 agtaaataaa atggaacaaa aagaagttgc actcagtgtt tcgaaagcca tcctcaacgg
    15181 agaatgggat aagttgaatg ggattttgtc cgatgatttt acctatacgg gcgatggaat
    15241 gttcctcaac aaagcgcagt acatggattt catgaaaggt atgagagctg catttagcaa
    15301 catgaacttt gaattctcac atgtgatcag cgagggcgat gcagtaagca tccgctttgt
    15361 tgtgaccgca aagcacacag gcagtttcat gggtggggcg gcgacgaaca aaaacctcaa
    15421 tttcagcgga attttcatac ggaaggtgaa gggtgataaa gtggtgcagg aatggcaagc
    15481 caccgacctg ctcggcatga tgactcaaat gggtgttggt accttgctcg gctacgccat
    15541 ctttggtgtg tggctcaaaa agaaataaca agataattgt ccccaaacaa aagcgtccat
    15601 ttcaacacga aatggacgct ttaccaatta ccaattacnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15721 nnnnnnnnnn nnnnnnnntt accaattacc aattactgct tcacgctgga accttatccc
    15781 attccttctg tgcgtgctca aagcctctct tcagggcttc atagttcttt ggcagcaagt
    15841 gcttgtgcct tgcaggtaaa tgagcctcca gcgctttttc cacgtcttcg atcttgatct
    15901 caggcagggc ggtgagcatt gctccgatgg ccaccatgtt catcaatttc ttgtcgccga
    15961 tttcttcagc gatttcattg cagggaacaa aaatcgtgtg gatatcgctg cgccgcgccg
    16021 aatgatccac catggactgg ttcacgatca acgttccgcc gctggccagc atgggttcat
    16081 acttatcgaa ggatggcagg ttgagggcaa ttgccaaagg tggattcttc accaatggcg
    16141 aaccaatttc atggtccgcg atgaccacgg tgcaattggc tgtgccgccg cgcatttcag
    16201 gtccatagga tgggatccaa gtcacatctc tgcctgtatc catggctgca tacgccagca
    16261 cctgaccgcc gaacaaaaca ccctgtccgc caaagcctgc gatgatgatt tctttttgca
    16321 tgttgctcct ttggagcaga gaatagtgaa tagataatgg agtgccgatc actattcact
    16381 aatcactact cactagattt ttatctgttt gatcgcttca ctgatcttgt aatcaccaag
    16441 tggataggcg gggaccatgc tgttttccac gaacttcagc gagttgacgg gagtcatgcc
    16501 ccaattcgtc gggcaggtgg aaagcaattc gaccatcgca aaaccaagtc cacgggcctg
    16561 ggtctcaaac gccatgcgga tcgccttttt cgccaggcgg atgttcttcg catcgtgtaa
    16621 tgaacggcgc gcacagaatc cgaccccatc cagtgtggcg agcatttcag ccattttgat
    16681 cgggtatccc tgcgtttcaa catcgcggcc attctgtgaa gaagtggtct tcatgccagg
    16741 cagtgtggtc ggcgccattt gtccaccggt catgccgaag ttggcattat ttacaaagat
    16801 cacggtcagg ttctcgcccc gtgccgccgc atggacaatt tcacccatgc cgatcgaagc
    16861 cagatcgcca tcgccttgat aggtaaaaac aattcggtca ggcagcacac gcttgatgcc
    16921 cgttgccatc gcgggcgcac gcccgtgcgg cgcttctaca aaatcggtat cgaaataatt
    16981 gtaggcaaac accgaacagc ccaccggcgc aacaccgatc gttttatcca aaacgttcat
    17041 ttcctgcagc acttctgcaa caagccgatg tgccacccca tgcgtgcaac ccgggcaata
    17101 atgggtgggt gattcggtaa acccttcagg tcttacacaa acaacatttt cagccagcat
    17161 tctgtctctc ctttaccctg ctgccatgcg ttcaagccac gcatcacgcg gattggcatt
    17221 cacggccatc ttgctttttg ccatgcggcg aatctcgttc agaatttcat cggggaaggg
    17281 aaccacgccg cccaatcttc catagaattc gaccggcaca cggcctttta cagccaggcg
    17341 gacatcctcc agcatttgac cggaattcat ctcagtgaca aggaacgcat ccactttacc
    17401 cgcaagctgc tcgatcaccg cagaaggaaa cgggctgaca gtgaccgggc gcaacaaccc
    17461 caccttgatt ccctgtgcgc gggcctcacg gaccgctgaa agcgccacac gtcctgctgt
    17521 gccaaagccg ataatcacaa tttcggcatc atcaaggaaa tattccttgt agcgtacttc
    17581 atttgcttga atggtccgcc agcgattgag caggcgcaga ttcatctttt cttcttcggc
    17641 aggctcaata taaatggatg aaagcacacg cctttcgcgt ttgcccatcc tgccatcggt
    17701 agcccaatcc caattggccc gctgcacggg tcgcatctca ggcatttggg caggctccat
    17761 catttgtccc accgaaccat caagaataac catgcacaca gagcggtatt tttctgccag
    17821 atcaaaggaa agcaccatca ggtcaatggc ttcctgcact gaagcaggag ccagcacgat
    17881 cggttgataa tccccatgcc cgccgccatg gacagcctgg ttgtaatcgg cctgagcagg
    17941 ggcaatgttc ccgaggcctg gtccgccgcg catcacgttg acaagcaccg cgggcacttc
    18001 cgtccctgcg atgtaggaga tgccttccat catcaaactc acccccggac ttgaagaaga
    18061 tgacatcaca cgcaccccgg tgcaggccgc gccatagacc atattgatcg cgccaagttc
    18121 cgattctgcc tgcacaaaag cacgtcccag ctcaggcatg cggcgggaga ggtattcaag
    18181 gatttctgtt tgtggggtga tcgggtaacc aaaatacgcc tccagccccg cgcgtacggc
    18241 agcttcggca atggcttcgt tccctttcca taattctttt ggcatttatt tctccattgg
    18301 tttttgtagt tcaaaagaga aagatgaaag aagaaagatg taaatttcct tcttctttca
    18361 tctttttatg cagttactgt aacgggggcg gcgttcttcg ttatttcacg atacacggtg
    18421 atcgctgcat ccgggcagac cagcgcacaa atagcacacc ccgtgcaacc gtccttgaac
    18481 gtgtgcgcgg ggtggtagcc cttcggggtc aggtgtgaca tgttaatttc catcacaccc
    18541 tgcggacatg cactaatgca cacctcgcat cctttacaat aagtatcact gacttcgatc
    18601 cagccttttg cggccattgc aactccttga tttatatgag aagtcggaca taatttagcg
    18661 ggattgcccg ccatgttgat tatgtcgttt tcctacaata aagagtagtg ccaagcgtca
    18721 cgaaggccaa agtacaaaca tcatataaag aaattcattt caaatacagg cccatctttg
    18781 caagccatct tccattcatg tggatgatgg gatgaaaccg cacacacgcc gcattctgcg
    18841 agacctccac acggcagtgg cgcctggatt aaaacctgcg cttcacgcac cgccgacacc
    18901 tgattcagtt tctcgaacag tttttttacc tgtggcagag tctcacgggc agaatcgact
    18961 gcgacgaaat cagcccattt gcaaatttct tcaagtgaac gcaacggctg cacttccacg
    19021 acttcgggca aaccactcgg cgcggaatcg cataccagca caacttcggc accctgtttc
    19081 aatgcaatgg gaatcaaccc gttcaggcgg gcggatgagg attcaagcgc gatcaacgcc
    19141 agtttgcgtg tattggcagg gacggaaaac ccatgcccaa gcgggccgcg taagttaagc
    19201 tgcgttccga ttgtccaggt agggggcagg ggcggcgcga agcgaaaccc tttcggcgct
    19261 gagtcgtaga agtaaacaga aacaggcagc ggtgaggttg atccgctggc gtgggcgagg
    19321 agatattgcc ccggcgctgg aatcagattt ggaggacagt ccatttgggc ggagccatcc
    19381 agatatattt cggtgattag tcctgtgcct gtgtgcatgg gatgattata atcaaccaaa
    19441 caataactcg gaggagtcat gaatattgca ggtgtaattc aagcggttgg ttcttttgca
    19501 tggctggttt tcgttggttt gctggtgatg atcggtgttc gttcgagccg caaccagcct
    19561 tcgaaggggt tcgggacagg ggtagtggca tcgctcgttg tagcgatttt gctgacgacc
    19621 cttggtgcag ggatcgtttt cattcagccc gaagaacgcg gcattgtgat ttctgcgttt
    19681 tcttccaaag ggtatcgtga acagtcattg cagcctggct tgcgctggat cattcctttt
    19741 gcagagcagg tcactttgta cccgatctcg cgccagacct atacaatgtc ttcatcgcct
    19801 acggaaggtc agattgtcgg cgatgattcg atccgtgccc gtacgaacga tgggcaggaa
    19861 attttgattg atgcgtcggt gatctactcc attgatccat cgaagatcat tgaactgcat
    19921 atcaactggc agagccgata tcaggatgaa ttggtgcgcc ccgcttcacg aaacaccatc
    19981 cgcgatgctg tttcgcaata cagcgtcgag gagatcgtca gcaccaagcg cgctgaaatg
    20041 gaagaagaga tcaccagatc gttgacagag gcattcaaag agaatgacct ggttttggtg
    20101 agtttcctgc ttcgtgatat tcattttagc gaagagtatg ctgccgctgt cgaacaaaag
    20161 cagattgccg agcaacaagc ccaacaggca aaattagttg ttgaacagaa aaagcaggaa
    20221 gccgaacaag cccgtcaggt tgcgcaagga caggcagatg ctgcggtgat tgcggcgcaa
    20281 ggcgcggcag aggcgcggct tattcaggcg caggccgagg cacaagccaa tcagttgttg
    20341 gcggcttcgc tcacgcccac gcttttacag tatcaatata ttttgaaact tgccccgggt
    20401 gtgcagacca ttttcattcc gagcggtaat cagtttattt tgccgctgcc cgataccact
    20461 ttgcagacac agtaaaatcg atcgcgcttc attggtacaa tatgaccgtc ggttgttttg
    20521 ccgatggtca tattgtttat aaggggcgca gactttagtc tgtgtgctga aagcggaata
    20581 aaatcttcat cccgctgatt cgaaatagga gcacaatgtc gattccacaa tctgggcgcg
    20641 aaattcgcct gaccacgctt tcggcgtgcg ctggctgagc gtctaaacta agcgctgatg
    20701 cgctgacgca ggttctgcgt ccgatcaaga atgtttttga tcctgctgca tatccagacc
    20761 ttttggtggg gcttgcttcc ccggatgatg cggctgtgtg gcgtttaagt gaagataagg
    20821 cgatcgtcgt cactacagat tttttcacac ccgtggtgga tacaccttac gaatatggtt
    20881 cgattgcagc cgccaactct ctttcagatg tatatgccat gggcggacag cccttccttg
    20941 ctttgaatgt ggccgccctg cccgatcatc tgccgattga aatctccagt gagatcatgc
    21001 gcggcggtgc ggagaaggcc cgtgaagcag gggtggtgat tgcaggcggg cataccgtca
    21061 aagacaagga accaaaattc gggttggtgg tgatcggttt tgtcgatccg cgaaagatga
    21121 tcagcaaagg cggtctgagg gtgggtgatg tgctggtgct gacaaaaccc ctcggcggcg
    21181 gtgtgacgac aactgcgctc aagcaggaaa aagctgaaga aaggcatatc gcggaagcca
    21241 ttgaatggat gtcgcgtttg aataaaacag cggggcagct tgccttggaa tttgatctgc
    21301 gcgggggcac ggatatcacc ggctttggtt tccttggtca tggcctggaa atggcggagg
    21361 cttccggggt acgtttgtcc atcgaaaatt cgaaggtgcc gctattgagc ggcgcacgtg
    21421 gttatgctga aaaagggacc ttccccggcg gcgcatttga caataaaggc catgtcggcg
    21481 aaaatgtccg cttcgatgtg ggtgttgatg aacctgccca aatgcttttg tttgacccac
    21541 agaccagcgg cgggcttttg cttggtgttc cgcgtgaaaa gctgagtgcc tttgaaactc
    21601 gtgccagaga attaggtcag cccgtttggg tggtcggtga agtgctggat gggcagggga
    21661 ttacggttca ataaagatgt ttcgcataag cattttggaa ttggctttca cctgtgctct
    21721 tattctgctg gttcttgtgg taccgattat ggtggcaagg tttcaggccc gtatggatgg
    21781 ccgtctgaaa gacattgaga agaagctcga taagaaaaaa caacaataag ttacaataga
    21841 agtagagaaa acgacctgaa aaaccccatg tcagaaattc ccatagaaat tttgatagac
    21901 ctttttgcgt ctccatttat caagttttat tcaaatcaat tgacgaaaga tgacttttgc
    21961 tgcgaggttt tttcgcggct ttaagttttt aagcctgtaa ccggtggaca aggaaaccat
    22021 gagtattatt aacgagatcg ttttgattct aattctgata ttgatcaatg cattgctggc
    22081 aatgtctgaa gcagcattgg tcgcttcccg caaggtgcgt ttgcagcaac aggcaaatga
    22141 cgggaataaa tcgtcagagt tggcgcttaa actgctcaaa gatcccaata tatttttatc
    22201 cacggtccag atcggcatta cgttgatcgg tgtgctggcg ggagcggtgg gtggtgccac
    22261 tatttcagag gccctggccc tggcgcttca gaagattccc tatcttggtg aatttagcga
    22321 atcaatttca ctggcaatcg ttgtgttgac catcaccatc ctcaccattt ggctgggaga
    22381 actggtcccc aagcggcttg gacttaccag cccggaaaag attgcgcagg ttgtggcagg
    22441 gcccatgctc ttcacttcga aggcgttttc tccgttgatt aaattaatga gcaaagccac
    22501 caactttgct ttgagcctga tgggagtgga cccttcttcc gaaccgcctg tgacggaaga
    22561 agagatccac ctgttgatcg atcagggtac gcaggcaggg gtgttcgaag aagcggaaca
    22621 ggatatggtt gagggcattt tcagcctcgg tgattcccgt gtttactcgc tgatgacccc
    22681 gcggaccgag gtggtttggt tggatatcaa cgattccatt gaggatatcc gtgagaagat
    22741 cgcagaatgt ccttactccc gttttcctgt tcggcaggat tcactggaaa ccatcgtcgg
    22801 catcgtcaaa tcgcgtgatc tactggtgga aagcctttcc ggtaaagctg tggatttgaa
    22861 gtctctgctc aagccagcac atttcatccc tgaaaccatg ttcgcttcac gcgcattgga
    22921 gctgttcaaa gagaaaaaca ccgaactgct cctcgtggtg gacgaattcg gtgggttgca
    22981 gggcttgctc acgatcaatg atattttgga ggagatcgtc ggtgccatgg aatttgagga
    23041 accgcaggcc acccaacgtc aggatggatc ctggcttttg gatggcatgt tggaggtgga
    23101 tgaattcaag gaaatcttcg atttcgagtc gctgccccat gaagatgaat acgaaaccct
    23161 gagcggattt gtgatgacct cgctcgggcg cgtcccgcag gcggctgacc atttcgagtg
    23221 gaataacttc cgctttgagg tgatcgacat ggatggccgc cgtgtggaca aggtgcttgt
    23281 taccacattg cccaaaccgc aaccgccaca ataaattccc ctgcaataaa tccctgctca
    23341 atgcagtttt acacggattt tcgcgaattt gatctgaaaa ttcgcggtca aaacttgtgc
    23401 ttttccgtca aaacggtgga gccataaaag gaaaaaaaca atgacagcct atgtaatcgt
    23461 agatattgaa gtttcagatc cagaggggta caaggagtat gtcaaattgg cgccggaagc
    23521 ggtgaaattg tatggcggaa agtacattgc acgtggcggc tataacgaaa ccctggaggg
    23581 tgattggcag gccaggcggc ttgtcattct tgaattccct tctgtagagc aggcaaagaa
    23641 ctgggtgaac tctcccgagt atgcaccagc ccgtgctttg cgtcataaat acgcccgcag
    23701 taacatggtc gtcgttgaag gcgtgtaatt gttcgatggt ataatttttt tcgtatgagc
    23761 caagaatcca accccaatca aaagacaaaa gtctgtccta catgcggtac gcgtttgagc
    23821 gaaaatgcaa tgcgctgtct cgtgtgtggc acggaattca acgccaaacc tgagccgaaa
    23881 gccgccaaga aagtggaaaa atcggtgcag gccagccgta tgccggtcat cactatgagt
    23941 ttaccggcgg ccgtgggcgc acttgcggtg gtggtcatcg tggccgctgc catcgtattc
    24001 ttctctctaa gggccagcgc accgcctgga acctttgaaa ctgctgaaac tgccacgccc
    24061 agcgccaccc ccaccgagac ccaacccgcc actgcgacgt tgattcccac ccaggtgcca
    24121 acctccacca atattccgcc tttcgattac accgtttctg cgggtgatgg aacctgttcg
    24181 cagatcgcgt tcaacttcaa tatttccgtt cagagcatca tcattgccaa taatctgcct
    24241 tccacctgtc cgatcagtgt ggggcaggtc ttgaagatcc cgtaccccac gccgaccgtt
    24301 ccgcctgctg caacaaatac ccccgaagca gccgaggcaa cccgcatcag ttgtgagacc
    24361 gttccgatca ccgtacagga tggggatact ctttcgacca ttgcgctgaa ttacgcggta
    24421 ccgccagagg ctattaagga attcaacggt ttacccaccg ataacgtctt tattggatcg
    24481 tcgctgctga ttccgctttg taagcgggca gccacccccg ggccgacccc aactgcgacg
    24541 attccgcctc cttaccccgc cccgaacctg cttctgcctg cggacggagc agccttcacc
    24601 ctggcaaacg acgtggtgac cctgcaatgg gcttccgtgg gtgttttgcg tgatggggaa
    24661 gcctaccaga tcaccattga ggatgtcacc tcagaccaaa cccgccgcct gaccgattac
    24721 gtgacggata ccaagtacat tgtcccaact tctttccgcc cgaaagataa tgtggcacat
    24781 gtcctgcgct ggtgggtgac ccccgtccgc caggcgggga ctgatgaaca ggggcagccg
    24841 atttgggttg cttccggtgc cgtcagcgac aagcacgtct tcacctgggt tggagtggcc
    24901 gtcgaaggca cgcccaatcc ctaggtccag accaagaagc ggattttcat tcccaaactc
    24961 cctgccaatt tcggtgggga gtttttcttt tccttgtgga attcccttga caaattcatc
    25021 cgcgtgatta taatatgtgt attattcaat aaattgaata aacagaataa agcggagcac
    25081 ccaatgtcac cttttgaact caacctcgaa aaaaccagaa ccgaaacttc acttgtaggt
    25141 cttgagcccg cgctcaatgc ttttgggagc atgctcctta tttcgaaggc agaggatgag
    25201 ccggggattc atgaatgggt ttcaaagacc cgtggagaga tgtcggcgga ggagaagttt
    25261 cgtcatggtt tggtgaccat cggtttttat tattcgattt tgccgcaaaa gggcggaatc
    25321 agcttcgaag catatttgga tggtttggag gcaacaccgc cttcggtatt tcgtgaacgc
    25381 ctgctcaagg cttatgaaat gatctgcatc acagaagagg caaaggccaa tcaaggcgag
    25441 tctgtgaact gggaagagac gctttcttct gctgaaaact atgtggactt tctgaaatat
    25501 cgttttggtg aagatctgac caatgtggaa atggagacgc gtgcctatca atatgtgatt
    25561 gaccctgccg cgctaaagca gctggtgacg ggtcacatcc gttggttttg gaagaatcac
    25621 ctgcaggcag aatgggcgcg tgttcgcccc atgttggaag aatcggccag ggcttttaat
    25681 aagctcgata ccgacgacat gacacgcctt gaacagttgc gatttgtgac cggcaaggat
    25741 gcgcaggaaa caaaatgggc agacaccctt gaaaatgccg accgggtgac ctttgtgccg
    25801 aacgctcata ttggcccgta tatgcatacg accagggttg gcaacgatct ttacatttat
    25861 tttggagcac acctgcccga aggctcagat gtgcagatac ctgaattgga ccgtgcggaa
    25921 attgtcagca aactttcggc tcttgccgat gagacacgtt tgcagatctt acaaatgatc
    25981 gcagaaaacg ggaaaatgcg ttccaacgag gtgatggatg caacccgact cagccagccg
    26041 agtgtttcgc gttacctcgg tcaactcaca gccagtggat ttttacagga aaggaggaca
    26101 ggcggcgcga aagaatattc tttaaatcgt gaacgtatcg agaagacact taaggcagtt
    26161 tcggcctttt tactggatcg gtagtcattg gaaggaggct taacatgaac ccgcttttgg
    26221 aagagtattt ggttgaagaa catcagagag agatcgaacg tgaaatgaac agcgcacagt
    26281 tgctcgggca gacggtgaag gcaggcgtat atcgcccgac attatccacc cgcctcctgc
    26341 gcggatttgg tcagtggatg atcgaaaaag gtgaagattt ggtgaaacgc catgagatcc
    26401 ctgctcaaaa tcggcgcacg gcagatcaac gctacgctca ttaatatttt tacggctttt
    26461 tcttcggaag cctgttgtta atgaaccacg aagacaggct tccgaagttt ttattctcaa
    26521 ggagagagaa tgttagcaag actcaagtct atttaccatg aatacccaaa ggcgttttgg
    26581 gtgtacaaca ttatcgtttt cattgaccgc ttcggcggat tcatgctcta tccgttcttt
    26641 gcgctgtacc tgacgaagaa gtttgagatc ggtatggcca cagtcgggat tttgtttggg
    26701 atcttttcca tttccgggtt ggtgggcagt gcgttgggcg gcgcaattac cgaccgaatg
    26761 gggcgcaagg gcgtgatcat tttcagcctt ttgctttcgg cattgagcgc acttggcatg
    26821 ggctatgccc ccacgctggg aatctttgtg gcggtttcgg tcatcgtggg caccctttcc
    26881 agcattggcg gacccgctca tgaggctgtg gttgccgacc tgctccctga aaacaaacgt
    26941 gccgaaggat atggcatcat ccgcgtgatc ttcaacatgg cggtcatcat tgctcctgcc
    27001 acggctgggt tgctcattgc aaaatcctac atcctgcttt ttatcattga tgcagtcatc
    27061 agtgtggtgg cggcggggat cgtccttttt gccctgccag agaccaagcc caaggctcac
    27121 cctgatgcga agcacgaaac cattgcccaa accttcgctg gctacggaaa agttttcaag
    27181 gacattccgt tcatgggatt cgtttttgtc accattttga tggcgctggt gtatatgaac
    27241 atgaacacca ctctcggtgt gtacttgcgc gatagccacg gcttgcctga attgaattat
    27301 ggctacctgc tcagcatgaa cgccctcatt gttgtgttct ttcaattttg ggtggcacgg
    27361 aaactggaaa aatacaaacc catgctgatg atggcgcttg gcaccgcgtt atacgcgatc
    27421 ggctttgcca tgtatggatt tacctcaacc tacctgctct ttgtggtggc gatgatcatc
    27481 atcaccatcg gcgaaatggt cgtttcacct tttcagcagg cgcttgtggc aagctttgcc
    27541 cccgaagaaa tgcgcggacg ttacatggcg atctcaggtc tttcatgggg catcgccttt
    27601 gcggctggcc cttacatggc aggctgggtc atggaaaaca tcaatccaaa ctggctgtgg
    27661 gttgcatgtg gcgtgatcgg cggcgttgcc atgctcggtt acattgtttt ggatcggatc
    27721 caccattccc ccgtatctat ggcggttgaa ccagccgctg cagattagca agttgttgtt
    27781 cccctcacaa gagaggcggg tctacgcagc ccgcctctca ccatttaatg ggctacggaa
    27841 tttgaattgg cacacttccc gccgcaaccc aggcctgtgc tgtttcttca atcgttgcaa
    27901 agcggagatt tggattctct ttcacctgct ccacgaaggc aagtatctca gtgaggccgt
    27961 cgtccgaatt gacgatcagc aaggtctcag gctcgatcat gattgtgaaa ctgagcatcg
    28021 gcacagaata tgctccagct tcgatctgtg caagcagcga ttcggctgtt gccagttgat
    28081 gatcggtatt ccccacacgc acaatgttac cctttgggtc atgcacggta tatttggcac
    28141 tgctggtcgg gcggtacaac acaatcgatg tatcgtcaca gcccgggcga tgacctatgc
    28201 aagccacccc accccaaacc actttcggcg tccatgtata acccttgtaa ctcgaaggtt
    28261 tccgtgcctt gtcgaactcg ctgatctgca ttccactgat aacgctggtg ggtgtggctc
    28321 cgtactttgc gagcaggtag gccgctttgg cgcggtcact ttgtttatgc gcgtgaacat
    28381 cccattgcac acccatggcg ctggtgcgct ggatgatctc ctgcacacgt atgccgcctg
    28441 tcaaccagcc cacatcggcc ccgatggaaa ggcgcacacc ggcatcggtc aattgctgga
    28501 tgaaggccag aaaggcatct acatccttcg gccaggaatc aggttgtgat tcaatatgca
    28561 tcgtagccga gacgtacagc atggttgtgg aagtggtggc agactttggc gattcattct
    28621 taaccgcagg ctgggtgacc gtttcaacgg gcagatccgc cgctgtcagt gattcgcctg
    28681 ccgctgctgc gggcgttccg caagataacg cgaacagggt gagaagggtg ataaatagat
    28741 acttgcgcat ggtggtctcc tatgttgtaa ttgtacggat aggttttatg cccccattgc
    28801 caccatgtaa aatctttgta acaaaaattc gaacaaggtg aggttttatg gatgcaaatc
    28861 atgatgtccg ttcttacaac cgcgcagctt gggataatca ggtccgttcc ggtaaaaacc
    28921 cgtggacggt gcctgtttct cctgaagtga ttgccaatgc ccgcaaaggg gattggtctg
    28981 ttttgctgac gcagaatatt cccgtaccga agaaatggtt cccgcccatg cagggattga
    29041 aaatcctggg gctggcgagc ggcggcggtc agcagggacc tgtctttgcg gcggcagggg
    29101 ccgaggtcac catttttgat aattctcccg cccaactgga acgcgaccgc gaggtagccg
    29161 accgcgatgg gttgaacatc cgcacagtgg aaggggatat gcgcgacctc tcggtatttg
    29221 ccgatgaaag ttttgacctg gtctttcacc ccgtctcaaa tttgttcatt cacgagattc
    29281 gccctgtgtg gaaagaggcg tttcgcgtgt tgcgcaaagg cggaacatta ctggcaggat
    29341 tcatgaatcc attgttctat ctttttgatt gggaagacat ggataacggc attttgaacg
    29401 tgagacataa aattccgttt tcagatatcg aaacctatgg catggaacga ttgaagcagg
    29461 aagaccgtcc cgctgagttt ggccattcgc tcaccgacca gttgacaggt cagatggagg
    29521 cggggtttca tctcattgac atgtacgaag atcatcatac gggcctgcgg atcagcgaat
    29581 tcaccccgac ctacatcgca acacgggctt taaaaccgta gggagtttca tgaaaatatt
    29641 aattgcagca gatatggaag gtattacagg agtggtacat tgggatcagg tcaaccccaa
    29701 gcaccctgaa tatgcccgtt ttcgcaaatt gatgactgcg gatgtgaacg ccgccattcg
    29761 cggggcgttt gctggtggag ccacatcggt ggtggtgacg gatggtcaca acagcggcag
    29821 aaatattttg atcgaggagt tggatgcacg tgccatgttg aacgctggtt cgcctgctcc
    29881 gctttcgatg gtgcagggtg tggatgacgg cgtggatggc gtgatgttca ttggctatca
    29941 cggacgtacg ggggcggccg atgcgattct cgaccatacg tggtcggaga gtgtctctgg
    30001 agtttgggtc aatgacaggg cttttgggga agtgggtttg aatggtgcgg tgtgcgggca
    30061 tttcgatgtc ccgatcatca tggcgtcggg cgatcaaacc ttgtgtgcag aagtgcgtga
    30121 attttttggc agtgaggttg aaaccgcaca agtgaagaag gctgttgggc gcatggcggc
    30181 tgaatgtttg acccctgcgg catctgccag tttgatcgag gaagccgcca aacgggcggt
    30241 gatcaacctg cgcagcggaa gaaagacaaa gccgttcaaa gtttctaaac ccatcaaaat
    30301 gacattggag ttcgctcaat ctgaaatggc ggataaagcc gcgctgatgc ctggtgccgt
    30361 gcggacagtg gatcggcggg tggaattttc cgcagaggat atggtgactg tgtatcttgc
    30421 atttcgagtg ttgatgacgt tggcagggta gggtggaaaa tagtaagtgg tgagtagtga
    30481 atagtgaata gtgaatagag agagaggatt atctatggcg aagaatgttg taaaggggtt
    30541 ggcggttttg ttttttctgg cgggcgggac ctgggttttg caaggcatca atgtgctgcc
    30601 aggcagtttc atgacgggtg atccgcagtg ggcgatcaat ggcggtatta ccatggctgt
    30661 cagtgcgggg gtgttttggt ttgcaagtcg aaaataatca tccaatttca caggaggcaa
    30721 tgatgagcga ttcgaaggtg aaacttgaac gcaaggcgaa aaagatgatc aagaatgaat
    30781 atgtcatctg gctgaccacg gtggatgcaa aacacgttcc ccaacccagg cctgtgtggt
    30841 tcatttggga tgaagattcc tttttgatct tcagccagcc gaaggcgcac aaggttcagc
    30901 acattcgtga aaatggcagt gtttccctgc atttcaacac agatgagaaa ggcgatcagg
    30961 atgtggtcat ttatatcggc atggccacat tggatgcgaa aacgtcgccc gcacataaag
    31021 tccctgctta tttgcggaaa tatcgcaaag gcatcaaaga gttggggatg actctggaac
    31081 agttcagcag tgaatattcg acggcgatcc gtgtcaaacc gacatcgctg cgcggctggt
    31141 gatgggctga aatggcagag tcgattcaaa taggtgatcg ggtaaagatc tatctcgatt
    31201 cgaaatttgg cgaaaaggaa ggctggtaca acggcaccgt cttcaaggtc gatccgtatt
    31261 cagcccatcg tagtttttac tgggtggaat tgaccacgga ggcacagacc ttgcttggaa
    31321 tcaggcagat ctctgtgttc aacttgaaga atatacagaa attacagccc gattcataaa
    31381 tgggcgggac gaccacagcg ggcagcggag ttcttctaaa gcactccgtg cccgctgtgg
    31441 tgaaaagaaa cggctaacga ttttttgctt ttaggcaggt ttgtaggctg tgatcttggc
    31501 gctgaagatg gctttataga tttcttcgcg tggatagttg ctcacgtcca agttcatttc
    31561 agcaatggct gattcgacga attccttgtc aaagaagacg ggggtgatgg aaatatcggt
    31621 gaagccgacg gaattcatca tggcgatgta ttcttctgcc tgcacggcgc cagctacaca
    31681 gccagcccag gcgctcaaac tttggcgcac aggttcaggc agaatacctg ctgtgacgat
    31741 atcagagact gccagtttgc cgccgggggc gagtacgcgg aaggcttcag taaaaacctt
    31801 ttccttatct ggtgagagat taataacgca attggaaatg atgacatcca ccgtgttgct
    31861 ttcaacggga aggtcttcga gatatccctg tcgaaactca acgttggtca aatggacctt
    31921 tttggcattg gctgtggcgc gttcgatcat ttcgggcgtc atatccacgc cgatgacatg
    31981 tccgctttcg ccgacttttt gtgcggcgag gatgcaatcg aggcctgccc cggagccgag
    32041 gtcgagcacg gtttgaccgg gcttcaatga ggcgagtgtg atcgggtcac cgcagccgta
    32101 gctggtgctg gccacgtcgc tgggaacggt ggtgagcagc tgttcggggt agagaatgtt
    32161 gtttggtgtg cagcagtcag aaggtccaca gcaggaagcg ctgcttttaa tccgctctgc
    32221 gtagtgttcg cgaacggttt cgtgaatggg agtgggggat tgggtcattt gagtctcctt
    32281 gttgaaaatc acataatgaa aaggttgtag gcaaagccga ggatgatgga accgacccac
    32341 agtgtgccga cgacgatgcc gatgacacgc cagcgggcaa tggtcagtgc tccgcccaac
    32401 gcgccgaggg atgtgcctgc tcctgtgatc aggaaggcaa gcgccgcacc ctgactcatg
    32461 ccgctatcga tcattgcgcg gacgagcggc agggatgctt cggtgttgat gtaaaacgga
    32521 agcccgagtg tggcggcaag tggaatgctg tagacatgcc cgctgccgaa caagctggtg
    32581 atccatgcgg cggggatcat tccgttgagc atgtaaccaa tgaaagtgaa gccgaggaag
    32641 agaggcagta agcgcttgct cacggtccac atttcttgta tgaacatttg aaaggtgacg
    32701 gttggctttt ctgtttttgc gacaggctgc atgatcaccg gcaggtcgag cgggaccggg
    32761 ccgcccatga ttcccattga ggtggcggca aatttgggag cagccatgcg ggcctggttc
    32821 gcaagccagc cacgtgattc ggcaaaactg gcaatggctc cgcctgcgag gccaagcaca
    32881 atggatgcga caaaaaaggc gatggcaaaa tcccagccaa aaaggccagc gctataaaaa
    32941 agctcctgcg gagaggtgag cggtgaggcc accaaaaagg ccacgatggg cgcccagggg
    33001 atcgtggaag ccatcattcc cagcacgatg gccgtggtgc cacacgagca aagcggtgtg
    33061 gcgacggcaa cgcctgtaga cataagcacg ctattctttg tgtttcggcg cagaaaacgc
    33121 gcaacggcat cttcatcaac ataaagtttc agcgcagcgc tggcgatgat gctcagcagc
    33181 agcaggaacc aattgtgaga aaaggtttcc acaactttgc cgaacgtgta ttgaagtaga
    33241 gatggaaagt ccattgtcag atcctttgta tagaagaatg tctatttgtt tttgtaaaaa
    33301 aatttaccga taaatgcgcc ctattcacca tgcggtgaac agggttcgta cactagcagc
    33361 agccgcaacc gcagctttca cagccgcctt cacagcattc attgtttaca actggtaatt
    33421 cgttcatggg gtttctcctt tcatatagaa gtttgtctat ggaatttgcc aaaaaaaacg
    33481 ggacgatgcc tcggccgaag acatttcccg atcaggcctt gatggtcttg atcaaatttt
    33541 ctgtggcgac agactccggt gcaaacttga ccatgagctg gccgcaacaa taagcgacat
    33601 tatcctggtt gagagagtag aacgtttgct tgccatcttc acgcacagtg accaagtccg
    33661 cttcgcgcaa aatggcgagg tggtgtgaca ccgtaggctg ggaaacatcg agcttctcaa
    33721 cgatctcgct gaccgaaagt gaactgcaac agcagatgtt catgatcttc tggcgggttt
    33781 cgtctgagat ggctttggca aagagaacag gattgaattt catttttgat ggctttctgt
    33841 tgtttattat atagacaaac gtctatttgt caagagttga aactgcccac ccggttttgc
    33901 tggggtgggc agtttttctg gtcgagagat atttggcttt gctcaatacg actttgcgca
    33961 gccagtcttt ccgaatttat tgcgaatcgt ccaccacgta ttcgtcgtca atggtgccat
    34021 cgccatcaag gtcaacacca aagtacatgc cttcggggtt ggcgtcattc cattcggcgt
    34081 agttgacgta gacaatggcg ccttcctgca aggcgatctc ttcggcggca aattcctctt
    34141 cgagttcgtc ggtgatgcgg gtgagataga aatcgaagaa gccttcgtta ttgagttttt
    34201 cggcattgat gagcaggtca ccggctttgg tgtcgagata gaccgtgatg atgccgccgc
    34261 cttgaatatc agcgccttgt acttcgaagt aatagtctgc ttcggcatcg ggattttcca
    34321 caccgatcac aatgaacggg gattcgtccg cttcggtttc gtaggcaatg gtttcttcag
    34381 acgggtagaa gtaagccgta tccacctggc caggttcgag gtagatgccc tccacaccga
    34441 tgctgaaacc ggcgccgatc atgatgaggt cggtgagtgt ttcttcggtt aaggcagagc
    34501 cgtcaattga aacggtcaca tccaaatcgg ctgggagcga gtaaatgggt tcgggtgcgt
    34561 ttccgctggc ttccatgcga tatgcagagt aggttgcacc gggtatctcg ttgacgatct
    34621 ttccatccac atagccgaga cggttgccgt tttcatcggt aatgagaatg tgaccttcgc
    34681 catcgagaaa gatctggttg atctggctgc cctgaagact cggtgaggtg agtttgccgc
    34741 ctgtaaaact tgaaactcca ctgccattac agaagggaca ttgttgggta ttgaggcggg
    34801 cggaagtggg ggtgaggtcg agggtttggg tatcggcatt gccagtgtaa agctcagact
    34861 gaacctgtgg attgatcgag gcttcgtaca gccaggtgtt gtcacgggta tccacataca
    34921 gttcgcgggt ttcgccgggg taattgttgt cgtatacgag aatggcgtac agaccattgc
    34981 ccttgtcttc cacaccaatg ggagtaatgg catgaccacc agaaccatct tctttgtaaa
    35041 ttccgatggt gtaggtttct ccttttgcat ccatttgttg gacgatctca aggatttcaa
    35101 caggagtgcc tttgataatg ctggtgttag taggcgctac agcttgagtt gcccaccaat
    35161 aggcaatttc gcgctggaga gcctcatcat tgatatcaag ttcgctggct gtggagcctc
    35221 caaagttaga tggtgagacc tggtcggtgt acatcattaa actgagtaca gccatgcctt
    35281 cacaatgccc gcctgccatg gcgccattga tctgttccat ccattgctcg gcaggaggtg
    35341 tcagggtgca ggtatctcca tcgaggcggg cgcagacctg gtcgccgaac atgcggcgca
    35401 cttcatccgc tgttagattt gtggcgggga tgtcatcgcc gtaattgcta aagctgaatc
    35461 cattgtccac tgggttaaag ccgagatcgt agacaacttc accagggagg ttggtggcgg
    35521 caggtgcccc accttctgat tctgaagcgg gtgcttctgt tgcaaagggt tcttcggtga
    35581 ggtattcatc attggtggct tcctctgtgt aatattcacc ttcttcgccg ccacacgagg
    35641 cgaggaagag gctgagaatc acaaaaagag agagcaggag gaacagggga ttttttctca
    35701 tgtgaacaga tcctttttga aaaaattttc tcccagtgtt attgggagaa tggtaaacgg
    35761 ggtgatgagg ttgccgtgct ggttaactgg attcgtctcc aagataccgc cttaccctcc
    35821 gcttgaggcg atagaaggag taccagagat tccaggcatg tttatgcggt gatgccagcg
    35881 aattcttata acggagtttt tcaagtttga ttttatcgaa ctttgggctt tcaagagttt
    35941 gcaaaaacct tgcttttgct gtgttataca cttcgatgtc aatggaatta agttctgcga
    36001 tgcggtcgag gtgatgctgt ttgaattcca gcaaacggct ggaatctttt ttgttcagcg
    36061 atgtgtaaaa tggtgtggac caattcagcg cagaggcaaa aagcaccagg ctttcgtcaa
    36121 aatactcctg caaaccgtat tcttccacct tgttgaacag attattcagc gccagatcgc
    36181 gcgcctcaga tttagacatc gaatcgatca acgtggaaag ggcgaatggt cccttgttga
    36241 tgagcatctt ggtctgtaaa ttctcaaggt cctttgtacc gcttgcgaga aactcatcaa
    36301 ggttgaaagg ccccttcaaa tactcaggcg attttccctc ggaaatatgc tgaataaaag
    36361 atttaacacg gctgatggga ttcctcaaaa aagtgatgat cttcgccgta tcggcttctt
    36421 ccagcccggt caagatcggc gcatggcctg tgaacagaac gaccttctgt ttttccgcct
    36481 cagggagttc cttgaatcgc atcacatcgt attgaaccgc acccttgaaa acaaaatgtc
    36541 ccttccccga atattgataa tcaaggcagg tggtaaatgt ggttcccgca gttttgggaa
    36601 tgtgcaggaa tctcaaacgc tccatgaagg tctccataaa agatttgccg cctgcaaggc
    36661 gggcaggatg gcaattataa gccaatgagt atcgcccttg caaaactgtc aataacacga
    36721 ttttttttag tacctacatc atggttgtta tccctgataa gttttataaa atttatgcag
    36781 agagagtgac tgggatcgtg ccccatatgc tttcgatttt gtcggttttt cccctccgtt
    36841 tagagaggtg acatgttttt tcagtaccca taaaattcca ccccaaggaa gaaaaggaaa
    36901 caaacaaatg aagaatatca gttttttccg ctctatttgt ttaagagtat ttctcagcat
    36961 tattttcctg acatccatca atgccgtccg catgaacgat ggccctcaaa gccccgccca
    37021 ggttgatgag gccgccgcca ccatcggaac ctgcccaacc ttcccgcagg ataacttttg
    37081 gaacacaccg gtcacaggct tgcccgttca ccccatgtct gatgcatggg tcagcgccat
    37141 tggcacaaac accggcttcc atatggattt cggttcaggc acatgggatg ggggaccgat
    37201 cggcattccc tacaacattg tttcgggttc cgctacaaca aaatatacag tggatttcta
    37261 ctacccggac gaatcagacc ccggaccgta tcccattcca aacagtccca accgtgaatg
    37321 gggttcggat catcatatcc tgatagtgga tactgacgat tgtacattgt acgaacttta
    37381 tgatgcttct caaaccacca atggatgggc cgctggctct ggcgcgatct gggatctgaa
    37441 ctccaatgcc ctgcgccccg atggctggac atccgccgat gcggctggtt tgccgatctt
    37501 gcccgggctg gcacgctatg aagaagtagc cacggggatt atcgaacatg ctttgcgttt
    37561 taccaccaac tgcaccgctg actattacat ctggcctgcg cggcacgtag cgcaatcagg
    37621 aagttgtgcg aacccgccgc cctttggtgc gcgcttccgc ctgaaagcgg attatgacat
    37681 cagcggcttt tcaccacagg cacaggtcat cttgcaggcg atgaagacct acggcatcgt
    37741 actcgcagac aatggctctc cctggtatgt gagcggtgcc cccaacgaag gttgggataa
    37801 tgatgtcctg cacgaaatgg acgtcttgac cggggccagc ttcgaggctg tggatacctc
    37861 tggcatgatg accacaacca cctttgccga tgtccccgag tcgcattggg catatcaata
    37921 tattgagcag ttatacgtcg caggcatcac ggggggctgt tcgaccaatc cattgatgta
    37981 ttgccccgat tcaaccgtaa cccgcgcaca aatggcgatt ttcattttgc gtggaatgca
    38041 tggcagtgcc tatg
//
LOCUS       DAOQLC010000027        37118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_332_length_37118_cov_23.227289, whole genome shotgun sequence.
ACCESSION   DAOQLC010000027 DAOQLC010000000
VERSION     DAOQLC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 37118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_37118_cov_23.227289"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PL000_06785"
     CDS             complement(<1..815)
                     /locus_tag="PL000_06785"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC88639.1"
                     /translation="MATVVEPTIFELSSPGRLGVTMPASDVPETALPPNHLLRSDLPL
                     PELAEVDVVRHYMRLSKFNYSVDDGFYPLGSCTMKYNPKINEDTCRLPGFLFTHPLQP
                     IETVQGNLALMFEMQEWLKEISGFAGVTLQPAAGAHGEFTGVLMMAAYHKARNDTKRT
                     KMLIPDAAHGTNPASVGMLGMSVVTIPSDSRGNVDLKALEAACDDTVVGMMLTNPNTL
                     GMFDENIEQVVKMVKDCGGLMYGDGANLNALLGIVRPGDLGFDVMHFNLHKTFS"
     gene            complement(815..2164)
                     /gene="gcvPA"
                     /locus_tag="PL000_06790"
     CDS             complement(815..2164)
                     /gene="gcvPA"
                     /locus_tag="PL000_06790"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNC88640.1"
                     /translation="MTYIPISPKERDAMLKTVGVKSLDDLFEAIPQKHRFPELDLPPA
                     LTEMEAATLLSEMAQTNENVREHMISFLGAGAYNHYVPSVVDHMLRRGEFYTAYTPYQ
                     PEISQGTLQAIFEYQSLMTNLTGMEVSNASHYDGATATAEAVNLAYAQFRGKRKKIVM
                     SPTVHPQYREVIRTYSQGMGLEEAGDDVAADLEAGPEELMSLVDDKTMLVVVQYPDFF
                     GRIHDYTKLIEDAHAKGALVCIVANPTALLMLKTPASMGADIVVGEAQPFGIPLWFGG
                     PYLGFFTTKKSYVHKMAGRLIGETVDARGQRSYVLTLTAREQHIKRERATSNICTNQG
                     LLALASAVYMSTLGKTGLQQVANLCYQKAHYAASELSKIDGFGLCFTEPFFHEFVLCC
                     PKPASEVNDYLLDNGIFGGYDLGKDYPSLKNHLLVAVTEMNSKQEIDTLVEVLKEME"
     assembly_gap    2180..2279
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2422..2799)
                     /locus_tag="PL000_06795"
     CDS             complement(2422..2799)
                     /locus_tag="PL000_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system H protein"
                     /protein_id="HNC88641.1"
                     /translation="MNAPSNLKYTKSDEWFDPASGAAGITDYAQNQLSDIVFVELLVD
                     EGETVEVGKPIASVESVKASAEIYAPASGTVSAVNKGLSEKPETLNSDPFGEGWMIKI
                     AGGSAGEVMDSAAYTTYCDGRAH"
     regulatory      complement(3029..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3268..3807)
                     /locus_tag="PL000_06800"
     CDS             complement(3268..3807)
                     /locus_tag="PL000_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88642.1"
                     /translation="MNTSYNNQKPGLLTAIIWMTLASGITNLFWGFVASATALGTIIG
                     VICLPITILPTILGVFEIIYAAKLLSAQPEPTKPSQTIAIFQILMILYGNVFSVVVGI
                     LALIFYNDQNVKDYFSRLNGVQVTTPDPMPVSPLRVEPEPLPEISPAEPVVPEIPALE
                     AEADPPVKPKRTRKSSTGK"
     gene            complement(3878..4774)
                     /locus_tag="PL000_06805"
     CDS             complement(3878..4774)
                     /locus_tag="PL000_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88643.1"
                     /translation="MRSSNRNSILYIVLALLTGLGLGLAYSWLLSPVTYVDATPAMLR
                     ADFKDQYRIVVAASYASSHDLARARARLQLLGDVDPVGQLSALAQNMVAGNASLEQVQ
                     PIAQLATDLQQGFSSSPVTDTPFVVVVNTPTEMDTPSAESLATETQIVLEEETPIPTL
                     EFDQTPLAPIVTSTFVPRPTFTPVPVSGDVFNLVGQDTVCDPGLQPGLLQFIIMDSRR
                     RQVAGIEIIVSWSEKEDRFFTGFKPELGDGYADFVMQPDVIYSVHVVEGGAIVPNIAA
                     PTCTDPNGISYFGGLLLTFQQP"
     gene            complement(4771..5130)
                     /locus_tag="PL000_06810"
     CDS             complement(4771..5130)
                     /locus_tag="PL000_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88644.1"
                     /translation="MKSSNWIMIIAAAIIGIGIGLTYGWVINPVVYTDVTPDALRADY
                     RADYVLMVAEAYQSDQNPETAARRIAMIGSASPDQIVEESLDYATANNFSQNEILLLQ
                     GLLTAMQTYQPQPVNAP"
     gene            complement(5161..5259)
                     /gene="ffs"
                     /locus_tag="PL000_06815"
     ncRNA           complement(5161..5259)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PL000_06815"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5325..6704)
                     /locus_tag="PL000_06820"
     CDS             complement(5325..6704)
                     /locus_tag="PL000_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC88645.1"
                     /translation="MDDRINILLIEDEDPHAELIQRAFEDQDARFQIFRVRSLTEARA
                     QLAEKDPALIIADWRLPDGESMELLPDHHDPLSTPIILMTSYGNERIAVEALKSGALD
                     YVVKSPESMLDMPHLAERAIEQWGARAERAKMQKALLESEAQFRLLAENASDMISRLT
                     TDGRVLYVSPACETILGYTPEELTGNSAFDIVHEDDRTFVRNLFKGNRYDATYTIAYQ
                     ALHKLGHYVWLESSAKAILDRKTNTISEIQIASRDITERKKSEKALQAAHEGLQEAYQ
                     RTIEGWVRALDLRDRETEGHTQRVTELTVKVARRLGFSEEELTHIRRGALLHDMGKMA
                     IPDEILQKPGPLNETEWERMRRHPLYAYEMLSPISYLHPALEIPLCHHERWDGSGYPR
                     GLKGEEIPLVARFFAIVDVWDALCSDRPYRKKMPQQEVMNYLREKSGILFDPKLVDVF
                     LSVVESEEK"
     gene            complement(6732..7175)
                     /locus_tag="PL000_06825"
     CDS             complement(6732..7175)
                     /locus_tag="PL000_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88646.1"
                     /translation="MNGEPVLVMLVEDNIDHAELVIRTLEDHRIANKIKHFMDGQTAL
                     DYLFRRGEYEDPKSSPRPHMILLDLRLPRVDGLEVLHQIKERDDLKNVPVIILTTSEA
                     EKDVARAYDNHVNSYLVKPVGFDEFSKLMNDLGFYWLGWNTHPRI"
     gene            7415..8167
                     /locus_tag="PL000_06830"
     CDS             7415..8167
                     /locus_tag="PL000_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88647.1"
                     /translation="MNNFFGVNLFAQLRNRIKAVFGVFTASISVFACGAFMTFVLAPN
                     QAILAYKVSQIPLMTAQSVESIAPGGDVLITGIINGNAPTPHLPEFIAYTEERWDVTV
                     SKNENGNDNTPSGSWTRVQTVVPLLTLDMDGTSVSIQASNTATLNGALHEKLVDGTGS
                     LQAVYEGQSLHDGAMRYKGLANGDLTTVFGKKASVGGVVPSQLFAGDRVAFEKYQRDL
                     ASSFLFMGITFMLCSPVVLVIGILGVLFGRKR"
     gene            complement(8172..9290)
                     /locus_tag="PL000_06835"
     CDS             complement(8172..9290)
                     /locus_tag="PL000_06835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC88648.1"
                     /translation="MLRNFLADLKDVSPACREYFLKADSLLSALAIGISAAVIPPTAV
                     LDYLYYGFTEKFFATVLFEGVFVALCLLAMVAVRHAHTVAVYDWAVFIWTFMAAIGFS
                     IMGFDQPERVTENILVVVIFTTVIYTLVTNRLLFRLIPAWMMMISYLVIFGSDLPTAI
                     IPQQEIFFSASLLVVNIGGLLIVGSSNRHKQDDYQARQSEKEARQQLETLASIDPLTN
                     IFNRRHFMEIFNHELDRGKRYGARFSLVLMDLDHFKQVNDTYGHLAGDEALKAFVRII
                     QENKRKTDIFGRLGGEEFGLLMMETSGADALTTLQRFLSKARQEHIVMDDSTFHFTFS
                     AGITETLPKDRSIDEIIHRADEALYKAKESGRNHCVLV"
     assembly_gap    9385..9484
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9568..10416
                     /locus_tag="PL000_06840"
     CDS             9568..10416
                     /locus_tag="PL000_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC88649.1"
                     /translation="MKSVVVTGVSTGIGWGVTKVLIQNGFRVFGSVRKTQDAERLKAE
                     FGDAFSPLIFDVTDEEKVHAAARQVREALNGETLFGLVNNAGIAIPAPMAHMPIAEFR
                     SQIEINLTGQLIVTQAFLPLLGMDHSLKGQPGRLINMSSTSGKRGAPFVGAYVTSKHA
                     LEGLSETLRIELMLYGIDVIIIGPGAVATPIWDKGEQADLTKYEQTEYIEAAKKFQRY
                     MVEDGRKGLPSEKIGEVVLHALTTPKPKVRYPVVPNMLKNWILPMLLPKRVFDNLIAK
                     GLGFRK"
     gene            10507..11244
                     /locus_tag="PL000_06845"
     CDS             10507..11244
                     /locus_tag="PL000_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88650.1"
                     /translation="MPNPLIELNNVSKTYSTAAGDFVALKEVNLKVEMGEFLGIVGKS
                     GAGKSTLLNMINGVDQLTSGEVIVSSNGKNVSIHDMNEDALALWRGKNMGVVYQSFQL
                     LPMLTLIENITLPMDLSNNFHPRRSKERAMELLRLVELEEHAYKLPAFISGGQQQRVA
                     IARALANDPPILVADEPTGSLDSITADHIFDVFEHLVSEQDKTIIMVTHDMGLASRFS
                     RSLTITDGEIATEDPEPQLDKTFRRRR"
     gene            11241..12380
                     /locus_tag="PL000_06850"
     CDS             11241..12380
                     /locus_tag="PL000_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC88651.1"
                     /translation="MNKTPLRTQPKSGPAQVADFAPESMIELRSVVKKFSNAAGEFTV
                     LKGVDLTINRGEFVSIVGKSGSGKSTLLNMITGIDHPTDGLVVVNGTDIYTGVSESQR
                     SKWRGKNLGIVFQFFQLLPMLTLLENVMLPMDYVDMYDFDERPTRAMELLKLVGLEKF
                     ANKLPVLVSTGQQQLAAIARAMACDPPLLVADEPTGNLDSRSADTIIRLFELFVSQGK
                     TIVMVTHDPSLTSRTNRNIIISDGELINETISRALPQLRHRHMLEFTRLAEERMVSPN
                     ETILQRGQAVEHFFMIRKGEVDLHGQTALRLRPRQFFGEIELLQGGGALTEVRAGNEP
                     VELLTIPRADFTRVIQESPITAEVLLKVAQEKMEALKEDKLAAKP"
     gene            12413..14311
                     /locus_tag="PL000_06855"
     CDS             12413..14311
                     /locus_tag="PL000_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88652.1"
                     /translation="MPDPRRTPQILYIEDSDETRSLVRRLLSKKYVVLEASDPLDGFQ
                     LAEETHPSLILLDNSLPHMSGIEAATRLSKMLPNTPIVVVSGDLNPGVRERALAAGAV
                     GFISKPIDADFVDQVDAYLNGRVEKLDNAEEHLQAYQQELVERLEGNIRQLTNTVEKN
                     KHLLQQNERMFSMLERRHKLLETAARVGQMVTSILDLDDLLKHTVNIICSEFSFYYSG
                     IFLVSEDGHWAVLRAGYAAAGRKMMAENYRLPVDEKSMIGKSIIQQQAQIALDVAGEE
                     SRFKNPFLPNTRSEMALPLIVKSRSLGGLTVQSDELNAFTEEDIAALQTMADQIAIAI
                     NNAQLMFKLEDATAELVRSKTFEAIATATGEAIHWVGNKAAPIPGSIKRAREDIRYIL
                     ALAAEYAPRDLDAKNVSLMEAVETIREEAQAMGIDLIQILAEMSAMKPNRLRALVSVE
                     SLMEDLDIAENSAETILSIKEGLIGPARQRNDAPVSLRDMIADTVANMGLPDGVVEMD
                     WADNVPNAFVDARQVEQVFNNLIKNAWEAMSQTPEPHIHVKGQRDSDPGFVLVTVKDN
                     GPGIPKEIQEKIWVSFFTTKGGSGGTGLGLSAVMQIVNQHGGSISLESEAGKGATFSV
                     RLPVEKKT"
     gene            14392..14781
                     /locus_tag="PL000_06860"
     CDS             14392..14781
                     /locus_tag="PL000_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC88653.1"
                     /translation="MTTVLLVDDEKLILRSLEKTLLRAGFDVITASDCKSGSETFAQN
                     MGEINIAVLDLNMPGFDGMPKTGAGLDLLADLKKQKASLPVIILTAYDEVTKAKDAVS
                     GGASAFFVKGREQGLVDLIQKTLQATG"
     gene            14836..16905
                     /locus_tag="PL000_06865"
     CDS             14836..16905
                     /locus_tag="PL000_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88654.1"
                     /translation="MTNDIHRHATLLKAAARAAKNITTILDPYDLLQRTVDIICDEFG
                     FYYAGVFFLDDAKQYAVLKAGRGEAGREMIHAGHRLAVGGNSMIGACIANRQGRIALD
                     VGAEAVFFENPHLPKTRSEMALPLVVGEDVIGALTVQSTEEAAFHDEDIAALQTMADQ
                     LAIAIQNSNLHRQAERRSRLLKAANRVGKEVTSILDLEKLLPQTVNIICESYGLYYAG
                     VFLVDEAGEYAVLRAGYGKAGKAMVADGHKLKIGTDSMIGACIAMSEARIALDVGEER
                     VHFKNPHLPHTRSEMALPLIYGGKVLGAVTIQSSEERAFSEDDITTLITMAEHLAVAI
                     NNARLIDELKEAHDEILRNKVFEALTTASTEAIHWIGNKTLPISLTVSRLQQEIAEGQ
                     VDVDSLREDLEMIAESAAQIIQVKEQLIGAVREQKPRPVLFADVVQTAAHQRDISSDM
                     LKVKIDPAAAYVIADSTQLVRAVGNILQNAAEAGAKSIRITAHETEERGFLEIMVDDN
                     GSGMDRDVMEKAWSPFFTTRGVDHHGLGLPAAMHVISQAQGRIAIVSEQGKGSTVAMY
                     LPKGILADEPLKTGSVKNLLLIDDNDDWANHFSNMLKGSNVKLTQTTDLKKLPAADLI
                     LVDENITSLPLAEVLDAIVKAGLAAKTVVLTSAINPERVTQFMHNGMKDVNVKPYSAG
                     EVSELLK"
     gene            16960..19392
                     /locus_tag="PL000_06870"
     CDS             16960..19392
                     /locus_tag="PL000_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88655.1"
                     /translation="MRPRWRKVLHDLWDNKARTLLVVFSIAVGVFSIGVIAGAYQIIS
                     NDMGISYAANNPSNIELRTANFDQDVLDNIRNMRGIEDAEARRVINFRARTIDSNAWV
                     SLDVIAFKSFKDNKVNLLKPVAGASEAKKDEILLDKRALEKFDFEIGDYLILELPDGT
                     SKQLRIAGIVQDSSSSAGDFLAPSLGFISMKTMPTLRQPDDLFNRAYITLSNGVDDSD
                     YIHEAGADIKDKLERNGTDVIRTRFSEKHKYPLADIITAILSILLALGILIVFLSSSL
                     IANTLDALLSQHLRHIGVIKLVGGRGRQVFAMYIALIMAFSFLALLVAVPSGGWGAYR
                     LAEFMAEKLRFDLLGYRIVPMALVIQVVIGLLVPLIAGIVPVVGGSRITVLRALSGDV
                     AGDENKPHKQTDRLNWFDWMLVRLTNMFARRGIHFPRPFIISIRNTFRRRGRLILTLF
                     TLTMGGAIFIAVFNVRVTLHNYMNDIGKYFRADVTLDFDKPYRLREVEQFALQIPNVR
                     SVEGWQFLSFELLHPDGAVAENVNVFAPPSDSTLVEPILVSGRWLEATDVRKLLISEK
                     VLDTYPGLQPGDPIFLKVNGHEETWEVIGLFKFVGQEGVFAYIPYEYAVQITNLPNQS
                     FSFRVVTLQHDRISQDQMAEQLDRFFRDEGFKVRKAESGLASLDSALESLDILVAFLL
                     IMAVLTAIVGAMGLTGTMGMNVLERTREIGIMRAIGADDRAVMRTIIAEGFVIGTISF
                     VLAIGVSIPFTYGLSYIVSLAVFETPIDVVFTYLGYAIWFGLVLFLSMLGSILPARNA
                     ARLTIREVLAYE"
     gene            19440..20567
                     /locus_tag="PL000_06875"
     CDS             19440..20567
                     /locus_tag="PL000_06875"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNC88656.1"
                     /translation="MKKQTLNIAAILVIFSLALAACGTQPATTPAPAEDVVTSNEVVA
                     EGRLEPIQGTNLSFQARGVVEEILVKAGDSVKQGDVLARLTNAGVAEAQVVIAQNAYD
                     TLLRNESGDRAKLWQAYMVAQEERGKAEQKWDDLNVDNIEDNIEDDKATVEDRKEDLQ
                     DALDEFEKYKDLDEDNSNRKAAEDDLERAQEDLNQAIRNLEEQIRERDEVRAKYDAAL
                     AVEAEAKYQYEISLDGPNADQLTLAKANLDSAKDTLSNYVIAAPFDGIVADVNIKAGE
                     QVTVDTRVLSIANFDSWVIETTDITELEVVKLSEGQPVTFVPDALSDVVLTGTVTEIS
                     EAFTQQGGDILYTVRIAVKETDPRLKWGMTVEVTFMEIVQE"
     regulatory      20689..20776
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      20868..20964
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            21118..22503
                     /locus_tag="PL000_06880"
     CDS             21118..22503
                     /locus_tag="PL000_06880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC88657.1"
                     /translation="MDINSPNFLFLFLPWFMLGYFFAPQRGKLIIGIAGSLLFYTWGN
                     LIYVPLMVTLTIFTYFLARGIEHWRGQKTAQILLWFGILINIALLVIFKLWTGIKYPL
                     GLSYVTFQVIAYLLEVYNKTSTSEKDLLAFSFYLLLFPKIPVGPITRYRQLREQIQNI
                     QVDPIEVAEGLRRYIRGFAKKALIADTLARVVTPIFKLASPTIAPSFAWLVIISYSLQ
                     LYFDFSGYTDMAIGLGRMMGFRFLENFNFPYISKSIGDFWRRWHISLSNWFRDFVFYP
                     LERRRLKWLGQPLNILIVFALTGLWHGLTRNFLIWGLIHGMALIFEATTLGKKLRTAW
                     APVQHLYALGVILVGWVFFRSPSPDFAFDFLRRLAGNMSGIQALPFETTSPLPFIEPT
                     FLLALAAGIIFSLPVGGWLQAKVDILTSTRPATRTPFQLAYDLGLFFLFLAALASTVS
                     AAFQPGIYGTF"
     gene            complement(22500..23645)
                     /locus_tag="PL000_06885"
     CDS             complement(22500..23645)
                     /locus_tag="PL000_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC88658.1"
                     /translation="MDRVRALVLSGGGGRGAFHAGVYKYLMESGKPKVDSAHSAQWDP
                     QVIVGTSIGAVNGAAIAQGMPADELVRVWELLEEKDIQGLPPGMRGLARWVAHKIFGT
                     IMDANLPQVSPQDATSPMPKEFWPPLPITSKWWGERLVGRWINLLDTGPLRKTLHTHF
                     KFDPREIASSQKTLLIAATNVQTGERMMFSNRAIQRRDTGEARQDVVTSITADRILAS
                     CSIPLVYPWTFDPETNAHYWDGALVANTPIGVALDAMRDVPIDVPAEIVVVLMTPWWQ
                     SGDPIPYTARKLPESFGDAITWTLDWMLLASFRERLRLIKAFNDLAVRERAEGAGPYK
                     YREVKVIIAAPDDFLPAERIIDYDGDVSRDLIQQGYAAAKKAFEAAF"
     gene            23889..25730
                     /locus_tag="PL000_06890"
     CDS             23889..25730
                     /locus_tag="PL000_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNC88659.1"
                     /translation="MKKMIRPFISSILGLLLLASCSFGSSDTQAAVAAQATSALPAVV
                     TLELTVQADTSVTYNTVGQVIKLTQNVRNSGSVSAPGPMIVTGAICPDMTTIGNLDAN
                     FDANETITCNSSYTITQADLDKGSVTITTMATVNNVNSNQVTTSIATVPPVVLKLTKT
                     AAPTTYSQAGQTITYSYVITNVGAATLGPAQFTVTDAAFAAPFNCGEATATLASNATV
                     TCTATYTITQADMGVASISTNATATGGGAPVSQAVGATVTKSDAAQPSGGQRTHTVVE
                     GEWLWQIARCYGVDPNKLAADNKDKIPDPSKIKPGVILTVNNPGGYSKFYGPPCITFV
                     SHTLQAGDTWATLGQKYSADATVLQLANPDTSLSAAGTVIKLRIPNYTMGTVTTPPAT
                     QSKTLSVTVAANPTAYNQTGQVITFTYTVKNSGTENLGPAQFTVSGGLLGSTPLNCGA
                     ASSTLAAGATLTCTSTYTVTQEDMTVNSISHQVTASGGGAGPSQAASVTVNKSTALLT
                     LTIAANPLTYTQAGQIITFTYAIKNSGSTNLGPAQFTISGGLHGATPINCGDAAATIL
                     PGGTLTCTASYTITEADLAVPTLSHLVIAAGGGAAPSQPASITLTKQ"
     gene            complement(25788..27584)
                     /gene="metG"
                     /locus_tag="PL000_06895"
     CDS             complement(25788..27584)
                     /gene="metG"
                     /locus_tag="PL000_06895"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNC88660.1"
                     /translation="MPENILIAVAWPYANAEIHVGNLTGSHLPGDIAARYHRLKGNKV
                     LMITGTDSHGTPVTMSADKLGKPVEEVYKSYHEGFIDLFQKIGISYDLYTTTHSENHF
                     KVSQTIFLALQKNGYLFKKTEPQWFSPGLNRFLPDRYIEGTCYICGTEGARSDQCDTC
                     GNVLDPMKLVNPKSKVEGDNSSPELRDTEHFYIDLSKLEPDVVKYLKDRADHMRDTVL
                     GESLRKVEAEGLKPRAITRDLDWGIPVPVDEAGWDTKKLYVWFEAVIGYLSAPIEWSQ
                     VSGNGEAWRDWWTNPNAKQFHFIGKDNIFFHTSLWPAELMGVGSAFMELFAGEEVPLI
                     LPYDVPANQFMNLEGKKISGSRSWAVWGRDALTRFDPDAIRYYLTINMPETKDSDWDW
                     AEFVARNNNELVATWGNLANRVLAFCYKNWEGHVPDVDLNTLRPADLELLAAIENGFN
                     TVGTELEAVHLRSAVGEAMKLATVVNQYLDVNAPWSAIKVDKVEAGKTIYTALKAIDS
                     LKVIFAPFLPFTSEKLHGFFNYETPLFGEQYTQEVSDSLGTHTVLRYKPVEGMQWKPS
                     DLKPGAKLNQPGPLFKKLDEIVSVEERARLGK"
     gene            complement(27826..29049)
                     /locus_tag="PL000_06900"
     CDS             complement(27826..29049)
                     /locus_tag="PL000_06900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNC88661.1"
                     /translation="MNSSIQKTLGFIVTLVLIGGMIFLRARLSYNTFDFQSSNFTFFW
                     LSGRMLIEGENPYDEKQYLAGHETYGIKWQPNRIFPYPLPLAIFFIPLGFLSLPAAYI
                     TWQVITLLIVATTIFLLLAHWQESAQRLLLVPVFAAMFFFGPMYLTLHAGSVGAIALL
                     ALLAAILLLEKDKSLLAGIVLSLTVLKPPQGVPILFLAGIWFLARRDWKAIYGMVIGG
                     IALLLIGMIQDPMWVVKFRGASEAVMDRTQGVHSNVWAFAYLACGGTSPCWTLLGAAL
                     SLLLLGAAGFFLWKNHPQGAAPAKWSAWEAFNLIIPISFVSTIYLWAYDQIPYLIPIV
                     WIIGVLVEKSRNFIYAFLFLIVLVLYSLFALVQQAGTDKDLWSLGTTLIVLGSLWLAS
                     RMKQKPPIDKPSSTA"
     gene            complement(29119..29628)
                     /locus_tag="PL000_06905"
     CDS             complement(29119..29628)
                     /locus_tag="PL000_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNC88662.1"
                     /translation="MPSPKKPFRINVGFIIYEEVGNNYDFPFEFNKIVLGDDLELRNF
                     EGIVNVGKTPQGLVVQADFSAETTLGCVRCLSDFDHELDWSFTELYAFDKRSETESAL
                     ILPEDAHIDLGELLREYALLEVPISPICKPDCKGLCTECGQNLNEKDCGHHEDLSDSP
                     FAKLKDLLK"
     gene            complement(29738..30703)
                     /locus_tag="PL000_06910"
     CDS             complement(29738..30703)
                     /locus_tag="PL000_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC88663.1"
                     /translation="MNAVTFLSPVTQEIKLVEERMRMQADESHPDLRAALEHLLVAGG
                     KRVRPTLGLLVGNMLNAPLDKLVTLGAAVELLHTATLVHDDLIDGALLRRGMPTLNAR
                     WSPPATVLTGDFLFARAAKLAAEADHLALMKLFSETLAIIVNGELTQMFTSRGLINRE
                     NYYKRIYAKTASLFEMTSRASAMVSPVDDGIIESMRDFGYQVGMAFQIVDDILDFTGE
                     QAAVGKPLGSDLHNGLVTLPAIYYAEANPDDADVLSLPNGGWTNNENMTRLVDNIRAS
                     DSSRLAMREAERHVDRALTILTTFENCAEREALENLAKYIVDRKV"
     gene            complement(30705..31526)
                     /locus_tag="PL000_06915"
     CDS             complement(30705..31526)
                     /locus_tag="PL000_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC88664.1"
                     /translation="MQLTDTHCHLDYHKFNPDRAEVIQRATDAGLIRLLVPGLHHRSS
                     KEAVKLAESNPSVYAAVGFHPTDLDEFTEKTFEKVKDLARHPKVVAIGEIGIDYYWVK
                     EMEKRAIQREVLKRQLQFAQEVGKPVVIHMREEGDAWTGDASVDLLAILSEWTSGLSG
                     TLKERPGVLHSFNGDLETAQKALDLNFFLGITGPVTYKTADKKREIIGQIPLDRILIE
                     TDSPFLSPVPHRGERNEPAYVRHIADKIAEIHLKSPAEIASITTANAARLFAWGD"
     gene            complement(31673..33352)
                     /locus_tag="PL000_06920"
     CDS             complement(31673..33352)
                     /locus_tag="PL000_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC88665.1"
                     /translation="MPRRSLVRLYRDEFANYSPAKFQQDLLAGLTVAAVALPLALAFG
                     VASGASAAAGLVTAVLAGFIMGLLGGAPYQISGPTGAMSAVLIVLVTRYGLEGIWVAG
                     LFSGFLLLLIGILRLGRFIAFIPAPVISGFTSGIALIIFIGQIDNFFGIKTAAAETAA
                     QKFIGYFTTPFSFDWHSVCLALVVIGAMLFWPARWNARFPASLLGIILATLLNWILSW
                     PVSMIGAIPQTLFLEQRLSFASIPWGNLSDFIAPTLTITALGAVESLLCGAVGSNMTG
                     VRLQANQELIAQGIGNMVIPFFGGVPATAAIARSSVGIKSGGQTRLVSVIHAVGILLS
                     MFLLTVFMERIPLAALAGVLMVTAVRMNEWDAIKFIFGRRFKTDIIAFTITMFATIVL
                     DLTQAILIGSFLAGAVFLNKIASIDITVQDVDIKRLQEKGIKTDGRCRHVRVAFLTGP
                     LFFAATGQFNEAFSNLKETHALILSMRGVSLIDTAGIEAIHRLHERLHKQGGTLMFAG
                     LHDNARQMMERGGLVETIGERNFFWSSDQAIVEAERRGCKFCRTRHKFHTV"
     gene            complement(33374..35098)
                     /locus_tag="PL000_06925"
     CDS             complement(33374..35098)
                     /locus_tag="PL000_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNC88666.1"
                     /translation="MPRRSLARLYKDEFSGYSFAVFQKDLIAGLSVAAVALPLALAFG
                     AGSGAGAAAGLITSIIGGLVIGALGGAPYQVSGAKAVTSVVLIVLVNRYGLEGMWFAS
                     LFSGLLMLTIGLLKLGRYIAFIPAPVIRGFTLGIALIIIIRQMDNLLGIHTPTTETAA
                     QKLIGYFNEDFIINPQAILLGLVVIVTMLLWPKKWNLYFPSTLAGLILATLLNQTLGW
                     SVTTIQSIPRTLILEQHLDLTKVPWTNLSDFIAPALTLTALGSIEAFLCGAVGSNMTG
                     IRLQVNQQLIGQGLGNITLPFFGAIPTTSAMIRTRVAIQSGARTRLVSIIHSLVLLIV
                     MFLFAPFLEQIPLAALAGLLMVVAIRTNEWDSIKRIFARRFKTDMIAFSIAMIATVVL
                     SLTDAILLGTFLAGAVFLNKIASIDISLQDVDVDRLKQRGIETAGKCKHIRVAFLTGP
                     LFFAATGQFEEAFANLKDTHALILSMRGVPLIDTEGLEEIHRLHERLRKQGGTLMFAG
                     IHDNARAMMERDGLVEAIGAENFFWSSDQAIVEAERRDCAFCAEEIAESPENAAAEDP
                     ITNNSSWR"
     gene            35333..36988
                     /locus_tag="PL000_06930"
     CDS             35333..36988
                     /locus_tag="PL000_06930"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88667.1"
                     /translation="MAAHIPSSMLLELGQAFSSMPELDPVLLSSLSQIQNIVASEGAA
                     LWLLNEAEVDIICTHATGADASKIMGSVMRARKFVAAYRTSTGRLTQTEDPLQSKWMD
                     SRAYRTYFNMSVRNMIGIPLVSRGKLLGEINIINKAGETAFTHNDYDFIHEVTQHIAL
                     AIQNTQLYERNNRTTGRQKLLNQISRHLHQTLDIDELIPRIFIEVNKAINAEAQSIWL
                     VDEEAGVIRCRFARGASAETLKGLSVPLNAPSIVGTSVSKQESIIIKDAQNDPRHARS
                     VDERTGFVTRSLMTVPLVLEDKSIGAIQAVNKRDGQLFTRDDLDLFRSIADSAALAVN
                     NAQLVADLQTSYDLTLDALSAALDLRDRETEGHSRRVVEYTTCLAQQIGLDKESIKNI
                     RRGALIHDIGKIGVPDAVLHKPGSLDETERNIINLHPLAGYNMLAGIPYLREEIQIVI
                     CHQEKWDGTGYPHGLRGEEIPIGARLFAIADTFDALTSDRPYRLGCSYETARGIIIEE
                     SGRQFDPRAVEAFLAIPEHEWMHIRAKVMEEIAHRRSLQKRVF"
BASE COUNT         9244 a         9850 c         9348 g         8476 t
ORIGIN      
        1 gagaaggtct tgtgcaggtt gaaatgcatc acatcgaagc cgaggtcacc gggacggaca
       61 atgccgagca gggcgttcag gtttgcccca tcgccataca tcaggccgcc acaatccttc
      121 accatcttca caacctgttc gatattctca tcgaacatgc cgagggtgtt ggggttggtg
      181 agcatcatcc ctacgacggt atcgtcgcag gcggcttcga gggctttgag gtccacattg
      241 ccgcgcgaat cagacgggat ggtgaccacg ctcataccca acatgccaac ggatgcaggg
      301 tttgtgccat gcgcggcatc ggggatgagc attttggtgc gtttggtatc gttgcgcgcc
      361 ttgtgataag ccgccatcat caacacgccg gtgaattctc cgtgcgcgcc tgcggcaggt
      421 tgaagggtga cgccagcaaa tccgctgatc tctttgagcc attcctgcat ttcgaacatc
      481 aatgcgaggt tgccttgcac agtttcgatg ggctgcaaag gatgcgtaaa cagaaagccc
      541 ggcaaacggc aggtatcttc attgatcttc gggttgtatt tcatggtgca ggaacccaaa
      601 ggataaaagc catcgtccac gctgtagttg aatttgctga gccgcatata gtggcggaca
      661 acatccactt cggcgagttc gggcaggggc aggtcagagc gcagtaaatg attgggaggc
      721 agggcagttt cgggtacgtc tgaagcgggc atggttacgc cgagtcgccc tggggaggat
      781 agttcaaaga tggtaggttc aacaacggta gccattattc catctccttt aagacttcca
      841 cgagggtgtc aatttcctgt ttgctgttca tttcagtgac ggcaaccaga aggtgattct
      901 tgagcgaggg ataatctttg ccgagatcgt agccgccgaa gatgccgtta tctagcaggt
      961 aatcgttgac ttcgctggca ggtttcggac aacacaaaac aaactcatgg aagaacggtt
     1021 cggtgaagca cagaccaaaa ccgtcgatct tgctcagttc gctcgcggcg tagtgggctt
     1081 tttgatagca aagatttgca acctgctgca agccggtctt cccaagcgtg gacatgtaaa
     1141 cggcggacgc gagtgcaagc aaaccttgat tggtgcagat gttggaagtg gcacgctcgc
     1201 gtttgatgtg ttgttcgcgc gcggtgaggg tgagcacgta ggagcgttgt ccacgggcat
     1261 ccacggtttc accgatcagg cggccagcca ttttgtgaac gtatgatttt ttggtggtga
     1321 aaaagccgag gtagggtccg ccaaaccaaa gcggaatgcc gaacggctgc gcttcaccaa
     1381 ccacaatatc cgcgcccatg gaggcaggtg tcttaagcat gagcaatgcg gttggattcg
     1441 caacgatgca aacgagtgcg cctttggcgt gcgcgtcttc gatcaatttt gtgtaatcgt
     1501 ggatgcggcc aaagaaatcg ggatattgca ccacgacgag catggtcttg tcatccacga
     1561 gcgacatcaa ctcctcgggg ccagcttcga ggtcggctgc tacgtcgtcc ccggcttctt
     1621 ccaggcccat accttgtgaa tatgtgcgga tgacttcgcg gtattgcggg tgtaccgtgg
     1681 gcgacatgac tatctttttg cgcttgccgc ggaattgagc ataagccaaa ttcactgctt
     1741 ctgcggtggc ggtggcgcca tcgtagtgtg aagcattcga cacttccatg ccagtgaggt
     1801 tggtcatcag cgattgatat tcaaaaatcg cctgcagggt gccttgtgaa atttcaggct
     1861 ggtatggagt ataggcggta tagaattcgc cgcgtcgcag catgtgatcc acaacagaag
     1921 ggacataatg attgtatgca cctgcgccga ggaaggagat catatgctcg cgtacatttt
     1981 cattggtctg cgccatttca ctgagcagcg tggcagcttc catttcagtg agcgcggggg
     2041 gcagatccaa ctctgggaaa cgatgttttt gcggaatggc ttcgaacaaa tcatccagtg
     2101 atttgacgcc gaccgttttg agcatcgcat cccgttcttt gggggaaatt gggatgtagg
     2161 tcatggtgcc tctttatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2281 cgcatcccgt tctttggggg aaattgggat gtaggtcatg gtgcctcttt atgcagttgt
     2341 ttgttgggag tttgtagttg ggagtgagtg gttggtcact tgctgccaac catcaaccac
     2401 ccaccatcaa ccgtcaacta attaatgagc ccgtccgtca caataagtgg tgtaggcagc
     2461 ggagtccatc acttcaccgg cagagccgcc agcgatcttg atcatccagc cttcgccaaa
     2521 cggatcggaa ttgagggttt cgggtttttc ggaaaggcct ttgttcacgg cgctgacggt
     2581 acccgaagca ggggcgtaaa tttcagccga agccttcaca gattcaacgg aagcgatggg
     2641 cttgccaact tccaccgttt cgccttcatc cacaagcaat tcaacgaaga caatatcaga
     2701 aagctgattc tgggcgtaat cggtaattcc cgccgcacca ctggcaggat cgaaccattc
     2761 atcagatttg gtgtacttta agttggaagg ggcattcatt gtcaatttct cctttgtgaa
     2821 atattttttt cagcgaatca aaaaaggacg cacgattcta caatcgtgcg tcctctgtaa
     2881 tggaacctga gagattagtc ggctggtcgc tttgtcccta gtctattcgt cgctttgtcg
     2941 caatgaccag cagactattc aactattgac catttgacca accaaacttt acaccgtcgg
     3001 tatcccgtgg gggattttcc agagttgcat cggaagcagg gtccttttgc ctgagagttt
     3061 catctggttt cgcctgtttg ccagatttgc accttcggcg ctcaactaca atgccgactg
     3121 aagtcgacgc tactgttgag tctcttccca aacatccgtt gaattatgct tccattttat
     3181 gagtgagggg aatgctagtc aaggtgtaaa acatcctgaa tttggataaa gaaagccgcc
     3241 tttttgaaag ggcggcttat tttcaagtta tttaccggtg gatgatttgc gcgtacgctt
     3301 cggcttgacg ggtgggtctg cttctgcttc gagggcagga atctcaggga caacgggttc
     3361 ggctggcgag atctctggca gaggttcagg ctcaacacgg agcggagaaa cgggcatggg
     3421 gtctggagtg gtcacctgca ccccgttcaa tctggaaaaa taatctttca cgttttggtc
     3481 gttgtaaaag atcaaagcaa ggatccccac caccacagaa aagacattgc cgtaaaggat
     3541 catcagaatt tgaaaaatcg cgatcgtctg tgatggcttt gtcggctcag gctgcgcgct
     3601 taagagttta gccgcataaa ttatttcaaa aacaccgaga atggtcggca ggatcgtaat
     3661 tggcaaacag ataaccccaa taattgttcc aagcgcagtg gctgatgcca caaatcccca
     3721 aaagagattg gtaattccgc tggcaagggt catccagatg atggccgtca gcaaacctgg
     3781 tttttgattg ttataagatg tgttcattcg tactccttga tcattttatg taagatataa
     3841 cgaatgaagc agggattggt tgcaaagcca gcctgcatta aggctgttga aaagtaagca
     3901 gcaatccgcc gaaataactt attccattcg ggtcggtgca ggtgggagcg gcaatattcg
     3961 ggacaatggc ccccccttca acaacatgga cactgtatat aacatcaggc tgcatgacaa
     4021 aatctgcgta tccatctcca agctcgggtt tgaagccagt gaagaaacgg tcttcttttt
     4081 cactccatga aacgatgatc tcgatgcctg ccacctgtct gcggcgcgaa tccatgatga
     4141 tgaattgcag caagcctggc tgaagcccgg ggtcgcagac cgtatcttga ccgacaagat
     4201 tgaagacatc accgctgact ggaacaggag tgaacgtggg gcgcggcaca aaagtggagg
     4261 taacgattgg cgcgagcggg gtctggtcaa attcaagggt gggaatggga gtttcttctt
     4321 cgagaacgat ctgggtttca gtggccagcg attctgccga aggggtatcc atttcagtgg
     4381 gggtgttgac cacaaccacg aaaggtgtat ctgtcactgg tgaacttgaa aatccctgtt
     4441 gcaagtcagt ggcgagttgg gcaatgggct gcacttgttc gagagaagca ttgccagcca
     4501 ccatattttg cgccaaggca ctgagctgcc caacggggtc aacatcacca agcaattgca
     4561 ggcgggcacg cgcacgggca aggtcatgcg atgaagcata ggaggcggcc accacgatgc
     4621 gatattgatc tttgaaatcg gcacgcagca tggcgggcgt tgcatccaca taggttacgg
     4681 gggagagaag ccacgagtac gccagaccaa gtccaagtcc cgtaagaagc gcgaggacga
     4741 tgtacaggat actgttacgg ttagatgatc tcatggggcg ttgaccggtt gcggttgata
     4801 cgtctgcatg gcggtgagca agccttgcaa aagcagaatc tcgttctggc taaagttgtt
     4861 tgcggtggcg taatccaaag actcttcaac gatctggtct ggggaggcac ttccgatcat
     4921 ggcgatgcgg cgggcggctg tttcggggtt ttgatcgctc tgataggctt cagcaaccat
     4981 gagcacataa tcggcgcgat aatctgcgcg caacgcatcg ggggtgacat cggtgtacac
     5041 cactgggtta atgacccagc cataggtcaa cccgatgcca atgccaatga tggcggcggc
     5101 aatgatcatg atccagttcg atgatttcat aagtgggagt gaaatgatgg ggacaaaata
     5161 tccgggcagg ctaccggcgg cacccgaaga ctcttcctta ccgttgctat ctttcgatcc
     5221 tggcggggtt cgaaaggctt cgccgcgccg ggcctgcccg gacgggggca aggatacccg
     5281 atgtactggg gcgtgtcaac atgagccatt caatgagtaa gatattattt ctcttcgctc
     5341 tccaccacag agaggaaaac atcaaccaat ttggggtcga acaggattcc tgatttctca
     5401 cgcaggtagt tcatcacttc ctgttggggc atttttttgc ggtaggggcg gtcactgcaa
     5461 agggcatccc aaacatccac aatggcaaag aaacgcgcaa caagtggaat ttcctcccct
     5521 ttcaatccac gcggatagcc gctgccatcc caacgttcgt ggtgacatag cggtatttca
     5581 agtgcaggat ggagatagga aatcggggaa agcatttcat atgcatacag cggatgacgg
     5641 cgcatccgct cccattccgt ttcattgagt gggcctggct tttgtaaaat ttcatcgggg
     5701 atggccattt tgcccatgtc gtgcagcaat gccccacggc ggatgtgcgt cagctcttct
     5761 tcagagaaac caagccttct ggccaccttc acggtgagtt ctgtgactcg ttgcgtatga
     5821 ccttctgttt cacggtcacg cagatccaat gctctcaccc agccttcaat ggtgcgttga
     5881 taggcttcct gcaaaccttc atgtgcggct tgcaatgctt tttcagattt cttgcgctcg
     5941 gtgatatcgc gggatgcgat ctgaatttcg ctgatggtgt tggtcttgcg gtccagaatg
     6001 gctttcgccg atgattccaa ccagacgtaa tgtccgagtt tatgcagagc ctgataggca
     6061 atggtgtagg tggcatcata acgattgcct ttgaataggt tcctgacgaa ggtacggtca
     6121 tcttcatgga cgatatcaaa ggcgctattg ccagtcagct cttccggggt atagccgaga
     6181 atggtttcgc aggcgggcga aacgtaaagc acacgtccat ccgtcgtgag gcgcgagatc
     6241 atgtcactgg cgttctcggc aagcaggcgg aattgcgctt cgctttcgag caaagccttc
     6301 tgcatcttgg cacgttcggc gcgggctccc cactgctcaa tggctcgttc tgcgagatgg
     6361 ggcatatcaa gcattgactc gggtgatttg accacatagt caagcgcacc agatttgagc
     6421 gcctcgaccg cgatgcgctc attgccataa cttgtcatta aaataatggg agtggaaagc
     6481 gggtcatgat gatcgggaag caattccatg ctttccccat cgggcaggcg ccaatctgca
     6541 atgataaggg cggggtcttt ttcggccagc tgggctcggg cttctgtcaa cgagcgcacg
     6601 cggaaaattt gaaaccgggc atcctggtct tcgaaggcgc gttggataag ttctgcgtga
     6661 gggtcttcgt cttcgattaa aaggatgttg atcctgtcat ccatatattg tgctttcaaa
     6721 attgagtaca gttaaatgcg agggtgcgta ttccaaccta accaataaaa tccgaggtca
     6781 ttcataagct tgctaaattc atcgaatcca actggtttaa ccagatagct gttgacatgg
     6841 ttatcgtagg cgcgggcgac atctttctcg gcttccgatg tggtcaggat aatcacagga
     6901 acgttcttca gatcatctcg ttccttgatt tgatggagaa cctcaagacc atccacacgt
     6961 ggcaggcgga ggtcaagtag tatcatgtga gggcgagggc tggatttggg gtcttcatac
     7021 tcaccgcgcc tgaaaagata atccagcgca gtctgcccat ccataaagtg tttgatcttg
     7081 ttggcgatcc ggtgatcttc caaagtcctg atcaccagtt cggcatggtc aatgttatct
     7141 tccaccaaca ttaccaacac aggttcaccg ttcatggttt tctccatagg tcttgccaat
     7201 tgtaaaccat ttgactcaaa ttggcatggg gctttcattt tcccaacttt gtcggttgga
     7261 aggatatctt tgctaggtca cagggcattc cataatttgg tacccaccgt ttttttctca
     7321 ctgcaatttt caaaaaagtt tactttatta cgaaacaagc gatagaattg cgaaaattta
     7381 tctgcaaaac atatcaattt tgggagccga tccaatgaat aacttttttg gagtcaatct
     7441 gttcgctcaa ttacgaaatc gcatcaaggc agtattcggg gtctttactg catccatttc
     7501 tgtttttgcc tgcggcgcat ttatgacctt cgtgttggcg cccaatcaag caattctggc
     7561 ctacaaggtc tcgcaaatac cgctgatgac cgcccaatct gtggaatcca ttgccccggg
     7621 cggtgatgtg ttgatcaccg gcattatcaa cgggaatgcg ccaacccctc acctgcctga
     7681 attcattgcc tatacagaag aacgctggga tgtgaccgtt tccaaaaatg aaaacggaaa
     7741 cgacaacacc ccatccggat cctggacccg tgtgcaaacg gttgtgcctc tgctaaccct
     7801 ggatatggat ggcacatctg tttccattca ggcatccaat acggccacac ttaacggggc
     7861 tttacacgag aaactcgtag atgggacagg ctccctgcag gcagtatatg aaggccagtc
     7921 tctacacgat ggggccatgc gctacaaagg tcttgccaat ggcgatctca ccaccgtgtt
     7981 cggcaaaaag gcctccgtcg gcggggtggt tcccagtcag ctttttgcag gtgacagggt
     8041 tgccttcgag aaatatcaac gggatcttgc ttccagtttt ctatttatgg gtatcacatt
     8101 catgctgtgc tccccagtgg tattggtcat cggcatttta ggtgtgttat ttggccgcaa
     8161 acgataatcg actacaccag aacacagtgg ttacgaccgc tttctttcgc cttatacaag
     8221 gcttcatcgg cacggtgaat gatctcatca atggagcggt ctttgggcag tgtctcggtg
     8281 atcccagcgc tgaaggtgaa atggaaggtc gaatcatcca taacgatatg ttcctgcctt
     8341 gctttggaga gaaatcgttg aagtgtagtc agcgcatccg ctcctgaggt ttccatcatc
     8401 aacaagccga actcctcacc tcccaaccgc ccaaagatat cggtcttgcg tttgttctcc
     8461 tgaatgatgc gcacgaatgc ctttaaagct tcatcccctg ccagatgacc ataggtatcg
     8521 ttgacttgtt tgaaatggtc gaggtccatt aaaacaaggg agaaacgtgc cccatagcgt
     8581 ttaccgcggt ccaactcatg gttgaaaatt tccatgaaat gccgacggtt gaaaatattc
     8641 gtcaacgggt cgatactggc gagggtttcc aattgctgac gcgcctcttt ttcgctttgg
     8701 cgcgcctggt aatcatcctg cttgtggcgg ttggaactgc cgacaatgag cagcccgccg
     8761 atattcacca caagcaaaga tgcactgaaa aagatctcct gctgcggaat gatcgccgtt
     8821 ggcagatctg aaccgaagat gaccagatac gagatcatca tcatccaggc agggatgaga
     8881 cgaaataaca ggcggtttgt aacaagggtg tagatcaccg ttgtaaaaat gacaacaacc
     8941 agaatatttt ccgtaacgcg ttcaggctga tcgaacccca tgatggaaaa accgatcgcc
     9001 gccatgaatg tccagataaa tacggcccaa tcgtaaacgg cgacagtgtg ggcgtgcctg
     9061 acagccacca tggcaagcag gcacaaggca acgaacaccc cctcaaacaa aactgttgca
     9121 aagaattttt ctgtaaaccc ataatacagg taatccaaaa ccgcagtggg gggaatgacc
     9181 gccgcagaaa taccaattgc caatgcagac aacagggaat ctgccttgag aaaatattct
     9241 ctgcaggcag gcgatacatc ctttaagtca gcaagaaaat tcctgagcat gggttgttga
     9301 tcggttaatt cgttctctgt tgtttaacct tttttctatt ttacctccct tgccctgctt
     9361 ttaaaccaaa cgatcagcct ggtgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnaggcaa gattataatc atttgactca attgaaattt tctctttccg tttatcgaac
     9541 gagttaaaaa atgaaaagga gacgcaaatg aaaagcgttg tcgtaacagg tgtttcaaca
     9601 ggaatcggct ggggagtgac aaaagtcctc atccaaaacg gattccgcgt gttcggaagt
     9661 gtgcgcaaaa cacaggatgc tgaacggctt aaagccgaat tcggagatgc attttcccca
     9721 ttgatcttcg atgtaaccga tgaagagaaa gtccatgccg cagccaggca ggtacgtgaa
     9781 gcactgaacg gggaaaccct ttttggcctg gtgaacaatg ccggcattgc catccccgct
     9841 cccatggcgc acatgccgat cgccgagttt cggagtcaaa tcgaaatcaa cctcacaggg
     9901 cagctcatcg tcacgcaagc cttcctcccc ctgctcggca tggaccattc attgaaaggc
     9961 cagccaggca ggctcatcaa catgagttcc accagcggca aacgaggcgc gccttttgtg
    10021 ggcgcctacg tcacttccaa acatgcgctg gaagggcttt ctgaaacact gcgcatcgaa
    10081 ttaatgctgt acggaattga cgtcatcatc attgggccag gcgccgtagc caccccgatc
    10141 tgggataaag gcgaacaggc cgacctcaca aaatacgaac aaacagaata catcgaggct
    10201 gccaaaaaat tccaaagata catggtcgaa gacggcagaa aaggcctgcc atcagaaaag
    10261 atcggcgaag tcgttttaca tgccctcacc acaccgaaac caaaagtccg ctaccctgtg
    10321 gtaccgaaca tgctcaaaaa ctggattctc cccatgctgc tgcccaaacg ggtttttgat
    10381 aaccttatcg ccaaaggttt ggggtttcga aaataggaaa attaccaaaa caatcaattg
    10441 aaaacccttt gcaaacccgt tatttgctac caattgcaca atagcggtaa aatcaagccg
    10501 aaaattatgc caaacccttt aattgaatta aataatgtat caaaaaccta ctccaccgcc
    10561 gcaggtgact ttgttgcgct caaagaagtc aaccttaagg tggaaatggg cgaatttctg
    10621 ggcattgtcg gtaaatcggg tgcaggaaaa tccaccctgc tgaacatgat caatggtgtg
    10681 gaccagctta cgagcggcga agtgatcgtc agttccaacg gaaagaacgt ctccattcat
    10741 gacatgaacg aagatgcact ggcactctgg cgcggaaaaa acatgggcgt ggtttatcaa
    10801 tcctttcaac ttctgcccat gctcacactc atcgaaaaca tcaccctccc catggatctc
    10861 tccaacaact tccacccgcg ccgttcaaag gaacgcgcca tggaattact gcgcctcgtc
    10921 gaactggaag aacatgccta caaactgcca gcctttattt caggcgggca gcagcagcgc
    10981 gtcgccattg cgcgcgcact cgccaatgac ccgcccatcc tcgtcgccga tgaacccaca
    11041 ggcagcctcg attccatcac tgccgatcac atcttcgatg tctttgagca tctggtcagc
    11101 gaacaagaca aaaccatcat catggtcaca cacgatatgg gcttggccag ccgtttttca
    11161 cgcagcctca ccatcaccga tggagagatc gccacagaag accccgaacc acaactggat
    11221 aaaaccttca ggaggcggag atgaacaaaa ccccgcttcg cacccagccg aaatctggtc
    11281 ccgcgcaagt agcagacttt gcgcctgaat ccatgatcga actacgcagt gtcgtcaaaa
    11341 agtttagcaa cgctgcagga gaattcaccg ttctcaaagg tgtagacctc accatcaatc
    11401 gcggtgaatt cgtttccatc gttggaaaat cgggcagcgg caaatcgaca ctgctcaaca
    11461 tgatcacagg catagaccac cccaccgatg gtctggtggt ggtgaatggc accgatattt
    11521 acacaggcgt ctctgaaagc cagcgctcga aatggcgcgg aaaaaacctg ggcatcgtct
    11581 tccaattctt tcagttactg cccatgctca ccctgcttga aaatgtcatg ctcccgatgg
    11641 attatgtgga catgtacgat tttgacgaac gccccacccg tgccatggaa ttgctaaaac
    11701 tggtgggcct tgaaaaattt gcgaacaaac tgcctgtgct ggtttccacg ggccagcagc
    11761 aactggccgc catcgcccgc gccatggcct gtgaccctcc cctgctggtg gccgatgaac
    11821 ccacaggcaa ccttgactca cgttccgccg ataccatcat ccgactgttc gagttgttcg
    11881 tgtcacaggg caaaaccatt gtcatggtca cccatgaccc gtcgctcact tcacgcacca
    11941 accgcaacat catcatctca gatggtgaac tgatcaacga aaccatttca cgcgcactgc
    12001 cgcaactgcg tcaccgccac atgcttgaat tcacccgcct tgccgaagaa cgcatggtat
    12061 cgccaaacga aaccatcctt caacgcggtc aggctgtgga gcattttttc atgatccgca
    12121 aaggtgaagt ggatctgcat ggacaaaccg ccctgcggct gcgccctcgt caatttttcg
    12181 gcgagatcga acttttgcag ggtggcggcg ccctgaccga ggtccgcgca ggaaacgagc
    12241 ctgtggaatt gctcaccatt ccacgcgccg acttcacacg ggtgatccaa gaatccccca
    12301 tcacagccga agtcctgttg aaagtggcac aggagaaaat ggaagccctg aaagaagata
    12361 agctcgcagc aaaaccataa agcatttcag gttttgaggc ttttttatga acatgccaga
    12421 cccacgtcgt accccgcaaa tcctttatat agaagacagc gacgagaccc gctcgctggt
    12481 aaggcgtttg ctttccaaaa aatatgttgt tcttgaggcc tctgacccac tggatggttt
    12541 tcagcttgct gaagaaacac accccagcct gattctgctc gataacagcc tgccgcatat
    12601 gtctggcatt gaagcggcca cccgtttgag caaaatgctg cccaacacac ccatcgttgt
    12661 ggtttcaggt gacctgaacc ctggcgtacg tgaacgggct cttgcagcgg gcgcggtggg
    12721 tttcatctcc aaacccattg atgccgattt tgtggatcaa gtggatgcct acctcaatgg
    12781 acgggtggaa aaacttgata atgctgaaga gcacttgcag gcgtatcagc aggaactggt
    12841 ggaacggctc gaaggcaaca tccgtcagtt gaccaacacg gtcgaaaaga acaaacacct
    12901 gctccagcaa aacgagcgca tgttctccat gttggagcgc aggcacaaat tgctcgaaac
    12961 cgcggcgcgt gttggacaaa tggtcacctc cattttggat ttggatgacc tgttgaaaca
    13021 tacggtcaac atcatttgta gtgagttcag cttctattac tctggcattt ttctggtttc
    13081 cgaagatggc cactgggcgg tgttgcgggc aggctacgcc gcagctggcc gcaagatgat
    13141 ggcagaaaat taccgcctgc ccgtcgacga aaaatccatg atcggaaaat ccatcatcca
    13201 acagcaggca cagatcgcgt tggatgtggc aggtgaagaa tcgcggttca agaatccatt
    13261 tttgccaaat acccgttctg aaatggcatt accgctgatc gtcaaatcac gctcccttgg
    13321 cggtttgacc gtacaaagcg acgaattgaa tgcattcaca gaagaagata tcgccgccct
    13381 gcaaaccatg gccgaccaga tcgccatcgc catcaacaat gcccagttaa tgttcaagct
    13441 ggaagatgcc accgctgaat tggtgcgctc gaagactttc gaagccattg ccaccgccac
    13501 aggtgaagcc attcattggg tgggcaacaa agccgccccg atcccgggca gcatcaaacg
    13561 tgcgcgtgaa gatatccgct acatccttgc gttggcggca gaatatgccc cgcgtgatct
    13621 ggatgcaaag aacgtgtcat tgatggaagc cgttgaaacg atcagggaag aagcgcaggc
    13681 aatgggcatt gacctgatcc agattctggc tgaaatgtct gccatgaaac cgaatcgtct
    13741 gcgggcactg gtcagcgtag aatcgctgat ggaagatttg gacatcgccg aaaacagtgc
    13801 ggagaccatc ctcagcatca aggaaggttt gatcggcccc gcccgtcaaa ggaacgacgc
    13861 gcccgtatca ctgcgcgaca tgatcgctga tacggtagca aacatgggcc tgcccgatgg
    13921 cgtagtggaa atggattggg ccgacaacgt gccgaatgcc tttgtagatg cgcggcaggt
    13981 ggagcaggtt ttcaacaacc tcatcaagaa cgcctgggag gccatgtctc aaacgcccga
    14041 gccgcatatc catgtaaaag gtcagcgcga ttctgaccct gggtttgttc tggtcactgt
    14101 aaaagataat ggccctggca ttccaaagga aattcaggaa aagatctggg tttcattctt
    14161 caccaccaaa ggcggcagcg gcggtacagg gctgggcctt tcagcagtca tgcagatcgt
    14221 gaaccagcac ggcggcagca tttcacttga aagcgaagca ggcaaaggcg caaccttttc
    14281 cgtgcgcctg cccgttgaaa agaaaaccta acggagtgca gacttcagtc tgcgtactga
    14341 tggtcgcggg ctaaagtccg cacttcgtga ataaattatt ttggaggacg aatgacaact
    14401 gtacttttag tagacgatga gaagttgatt ctacgaagcc tggaaaagac ccttttgcgc
    14461 gcaggcttcg atgtgatcac agcctctgat tgcaagagcg gaagcgaaac ctttgcccaa
    14521 aacatgggcg agatcaatat cgccgtgctg gatttaaaca tgcccgggtt cgatggtatg
    14581 cccaaaaccg gtgccgggct ggacctgtta gctgatctca aaaaacaaaa ggcatctctg
    14641 cccgtcatca ttctgactgc gtacgatgaa gtgaccaagg ccaaggatgc cgtttccggc
    14701 ggagcaagcg cgttcttcgt caaaggtcgc gaacagggtc tggtggattt gatccaaaag
    14761 acattacaag ccacaggcta gtcctttaac tttcaacttg taaaccttca aacacttaat
    14821 ccaaaagagg aaattatgac aaacgatatt caccgacatg caaccttgtt gaaagctgct
    14881 gcgcgagctg caaagaacat caccaccatc ctcgacccct acgacctgct ccagcgcacc
    14941 gtagatatca tctgcgatga gtttgggttt tattacgcag gcgtattctt cctggatgat
    15001 gccaagcaat atgctgtcct caaagcaggg cgcggcgaag caggcaggga aatgatccac
    15061 gccgggcatc ggctcgcagt cggcgggaac tccatgatcg gagcctgtat cgcaaaccgc
    15121 caaggacgca tcgcgctgga tgtgggcgcg gaagcggtgt tctttgagaa cccccacctt
    15181 ccgaaaacac gttcagaaat ggcacttcca ttggtcgtgg gtgaagatgt catcggtgcg
    15241 ctgacggttc aatccaccga agaagctgcc ttccatgatg aagatatcgc cgccctgcaa
    15301 accatggccg accagttggc gattgccatt caaaactcca acctgcaccg tcaggccgaa
    15361 cgccgttccc gtttgctaaa agccgccaac cgcgtcggca aggaagtcac atccattctc
    15421 gatctggaaa aacttcttcc gcaaaccgtc aatatcattt gcgaatccta cggcttgtac
    15481 tacgcgggcg tcttccttgt ggatgaggcc ggtgagtatg ctgttctgcg cgctggctac
    15541 ggcaaggcag gcaaagccat ggtggcagat ggtcacaaac tcaagatcgg aaccgactcg
    15601 atgatcggtg cctgtatcgc catgagcgaa gcccgtatcg cattggatgt gggtgaggaa
    15661 cgggtacatt tcaaaaatcc gcacctgccg cacacccgct cagaaatggc tctgcccctg
    15721 atttacggcg gcaaagtcct tggcgcggtg accattcaaa gctccgagga acgtgccttc
    15781 agtgaagacg atatcaccac attgatcacc atggccgaac atctggcggt ggcgatcaac
    15841 aacgcccgcc tgattgacga actcaaagaa gcacatgatg aaatccttcg caacaaagtc
    15901 tttgaagcac tcaccaccgc ctccactgaa gccattcatt ggatcggaaa caagaccctg
    15961 ccgatctctt tgaccgtttc ccgcctgcag caggaaattg ccgaaggcca ggtggatgtg
    16021 gactccctgc gagaagacct tgagatgatc gctgagagcg ccgcacagat cattcaggtc
    16081 aaggaacaat taatcggtgc cgtgcgcgaa cagaagccgc gccctgttct gtttgctgat
    16141 gtagttcaaa ccgcggccca ccaacgtgat atttccagcg acatgctcaa agtgaaaatt
    16201 gaccctgccg ccgcatatgt cattgccgat agcacccaac ttgtccgcgc tgtgggtaat
    16261 attttgcaaa atgccgccga agctggcgcc aaatccatcc gcatcaccgc acacgaaacc
    16321 gaggaacgcg gtttcctcga gatcatggtt gacgacaacg gttcaggcat ggaccgtgat
    16381 gtcatggaaa aggcctggtc tccattcttc accactcgcg gcgtagacca ccatggtctt
    16441 ggcctgccag cggccatgca tgtcatttca caggcgcaag gccgcattgc catcgttagt
    16501 gaacagggca agggttcaac ggtcgccatg tacctgccaa aaggcattct ggcagatgaa
    16561 ccgctcaaga ccggcagtgt gaaaaacctg ttgttgatcg acgacaacga tgattgggcc
    16621 aatcattttt caaacatgct caaaggctca aatgtcaaac tcacccagac cactgacttg
    16681 aagaaattgc ccgctgccga cctgattctg gtggatgaaa acatcacatc ccttccgctg
    16741 gccgaagtat tggatgccat cgtcaaagcc gggcttgctg caaagaccgt agtcctcacc
    16801 tcggcgatca accccgaacg ggtaacacaa ttcatgcaca atggcatgaa agatgtgaac
    16861 gtaaaaccct actccgccgg ggaagtaagc gaattattga agtaatttat atcaagacct
    16921 gacaggcctt tcacgacctg tcaggtctct gtgagtaata tgcgaccaag atggcgtaaa
    16981 gttttacatg atttatggga taacaaagcc cgcacactgc tggtggtatt ctccatcgca
    17041 gtgggcgtat tttccattgg ggtgatcgca ggtgcgtacc agatcatcag caatgacatg
    17101 ggtatttcct acgcagccaa caatccgtcc aacattgaac tgcggactgc gaatttcgac
    17161 caggatgtat tggacaatat ccgcaatatg cgtgggattg aagatgccga agcaaggcgg
    17221 gtaatcaact tccgggcacg caccattgac agcaacgcct gggtatcact ggacgtgatt
    17281 gcgtttaaaa gtttcaagga caataaagtc aatttattga aaccagttgc aggggcgtct
    17341 gaagcgaaaa aggatgagat cctgctcgat aaacgggccc ttgaaaaatt cgattttgag
    17401 atcggcgact accttatcct agaattgccc gatggcactt caaagcaatt gagaatcgct
    17461 ggcatcgtcc aggattcaag ctccagcgcc ggggactttc tggcgccttc gcttggtttc
    17521 atttccatga aaaccatgcc caccctgcgc caacccgatg acctgttcaa ccgcgcatac
    17581 atcacccttt ccaacggcgt ggacgattcg gattacatcc atgaagcagg cgcagatatc
    17641 aaagacaaac tggaaagaaa tggcacagat gtcattcgca cccgcttttc tgaaaagcac
    17701 aagtacccgc ttgcagatat catcactgcc attctcagta ttttgctggc actcggaatc
    17761 ctgatcgttt tcctttcaag ttctctgatc gccaacacac tcgatgcact gctaagtcaa
    17821 cacttgcgcc atatcggtgt gatcaaactg gtgggcggac ggggcaggca ggtctttgcc
    17881 atgtatatcg cgctcatcat ggcattcagt tttctcgcct tactggttgc cgttccttca
    17941 ggtggatggg gtgcataccg tctggccgaa ttcatggctg aaaaattaag gtttgacctg
    18001 cttggttatc gcattgtgcc aatggcactt gtgatccagg tcgtcattgg cttgctcgtg
    18061 ccattgatcg cagggattgt gcccgttgtg ggcggatcgc gcatcacggt cctgcgcgcc
    18121 ttgagcggag atgtcgcagg cgacgagaac aaacctcaca aacagactga ccgcctcaat
    18181 tggttcgact ggatgctggt acgcctcaca aacatgttcg ccagacgcgg cattcacttc
    18241 cctcgcccct ttatcatctc catccgcaac acattccgcc gccgcggacg tttgatattg
    18301 accctcttca ctctgaccat gggtggcgca atttttatcg ccgtgttcaa cgtgcgcgtc
    18361 actttgcaca attacatgaa cgacatcgga aagtatttcc gtgccgatgt cacactcgat
    18421 ttcgacaagc catatcgcct gcgggaagtg gaacaattcg ccctgcaaat cccaaatgtc
    18481 cgctcagtgg aaggttggca attcctgtcg tttgaactcc tgcaccccga tggagcagtg
    18541 gcggaaaacg tgaatgtctt tgctccccca tcagacagta cattggttga gccaattctg
    18601 gtttcagggc gatggctcga agccacagat gttcgcaaat tgttgatcag cgagaaagtg
    18661 ctcgatacct acccaggcct tcaacccggt gaccctattt tcttgaaggt caacggccat
    18721 gaggaaacct gggaagtgat cgggctgttc aagtttgtcg gtcaggaagg tgtatttgcc
    18781 tacatccctt atgaatatgc cgtccagatc accaacctcc ccaaccagtc tttttccttc
    18841 cgcgttgtca ccttgcagca tgaccgcatt tcccaggatc aaatggccga acaactggac
    18901 cgtttcttcc gtgatgaagg cttcaaagtc cgcaaggctg aatcagggct tgcatcactg
    18961 gatagcgcgc ttgaaagcct cgacattctt gttgcatttt tgctcatcat ggccgtactt
    19021 accgccattg tcggcgccat ggggttgaca gggaccatgg gcatgaatgt ccttgagcgc
    19081 acccgcgaaa ttggcatcat gcgcgccatc ggcgcagatg accgtgccgt catgcgcacc
    19141 attattgcgg aaggttttgt gatcggtacg atctcatttg ttctggcaat tggggtttcc
    19201 atcccattca cctacgggct ttcctatatc gtcagtctgg cggtgtttga aacccccatt
    19261 gatgtggtct tcacttatct gggctatgcc atctggtttg ggctggtgtt attcctttcg
    19321 atgctcggct ccattttgcc cgcccgcaac gccgcccgcc tgaccattcg agaagtgctg
    19381 gcatacgaat aaaaaaagtg ccgcaccaac ggcatccata ataagaaggt aaataaataa
    19441 tgaaaaaaca aactctaaac attgcagcaa tacttgtcat tttttccctc gcccttgcgg
    19501 cgtgtggaac ccaacccgca accacccctg cccctgcaga agatgtagtt acctccaatg
    19561 aagtggtggc agaaggccgc cttgaaccca ttcagggaac caacctttcc tttcaagcac
    19621 gcggcgtagt ggaagagatc ctcgtcaaag ccggagactc tgtcaaacaa ggtgatgtcc
    19681 tcgcgcggct gacaaatgcg ggcgtggctg aagcgcaagt cgtcattgca caaaatgcct
    19741 acgacaccct gctccgcaac gaaagcggtg accgggccaa actctggcag gcttacatgg
    19801 tcgcgcagga agaacgcggc aaagctgaac agaaatggga cgacctgaac gtggataaca
    19861 tcgaagacaa tattgaagat gacaaagcca ctgtggaaga ccgcaaggaa gacctgcaag
    19921 acgcattgga tgaattcgaa aaatacaaag acctcgacga agataattcc aaccgcaaag
    19981 ccgctgaaga cgatctcgaa cgggctcagg aagatctcaa ccaggccatt cgcaacctcg
    20041 aagaacaaat ccgcgaacgc gacgaagtcc gtgccaaata cgatgccgct ctcgctgttg
    20101 aagccgaagc aaaatatcaa tacgagatca gccttgatgg ccccaacgcc gaccaactca
    20161 ctcttgccaa agccaatctc gattctgcca aagacaccct ttccaattac gtaatcgctg
    20221 cacccttcga tggcatcgtg gccgatgtca acataaaggc aggcgaacaa gtcaccgtgg
    20281 acacgcgtgt gttgagcatc gcaaacttcg attcttgggt catcgaaacc accgacatca
    20341 ccgaactcga agttgtaaaa ctgagcgaag gccaacccgt cactttcgta cccgatgccc
    20401 tgtccgatgt cgtcctcaca ggcacggtca ctgagatcag cgaagccttc acccagcaag
    20461 gcggcgacat tctctacacc gtccgcattg cggtaaagga aaccgatccg cgcctaaaat
    20521 ggggcatgac ggtggaagtc accttcatgg aaatcgttca ggaataaata tttacaaagc
    20581 cagattcaaa atcattccgc cgccatttct ggcggcggaa tgtcgtttaa ataaatagaa
    20641 catctgagtg attgaaaata tttttcgagc gtgcaataat ttaattgctc cgaaaaaagg
    20701 caaaaccggg gaaaccccgg tcacgcaaag tcatgggcct aaagctgaac tgagtcatgg
    20761 cagccagact accgataaaa gcaaaaccgc caaaagacgg cgacgcagag tcagagcgtc
    20821 taaaatcagc aagtacgcac gttgtacagt tgatcaagac cgaccgaccc tccgaaacgc
    20881 aaaatcagga aaacctgatg acgcaaagcc acgggtctaa aatcacaact ttatttttgt
    20941 gatcatgatc gccaggctgc cgaagagcaa atcttttctc agggatgacc ataatttggg
    21001 tcatccctgt ttttttgcgg atgtaaaacc gcctcccact tacccgaaaa ctgggaggcg
    21061 gtttattgtt ataatcgcgc cgacacattg tcccctccac agggcaggcc aacttttatg
    21121 gacattaatt cccccaactt tctttttcta tttctgccct ggttcatgct gggttacttc
    21181 ttcgcccccc agcgtggaaa acttatcatc ggcattgcag gcagtttgtt attctataca
    21241 tggggcaacc tcatctacgt cccattgatg gtgacactga ccatattcac ctattttctg
    21301 gcacgcggca tcgagcattg gcgtggacaa aaaacagcgc aaatcctgct atggtttggg
    21361 attctgatca atatcgcatt gctggtcatt ttcaaattat ggacgggcat caaataccca
    21421 ttggggcttt cctacgtcac atttcaagtg atcgcctacc tgcttgaggt ctacaacaaa
    21481 acatccacaa gtgaaaaaga cctgcttgct ttttcctttt accttttgct cttccccaaa
    21541 attcctgttg gcccgatcac ccgttaccgt caactccgtg aacagataca aaacattcag
    21601 gttgacccga tcgaggtggc agaaggtctc cgccgctaca ttcgcggatt cgccaaaaaa
    21661 gccctgatcg ccgataccct cgcaagggtg gtcaccccga tcttcaagct ggcatccccg
    21721 accatcgcac catcgttcgc atggctggtc attatcagtt attccctgca gctatatttc
    21781 gatttttcag gatacaccga tatggccatc ggcctcgggc gcatgatggg ctttcgcttt
    21841 ctggaaaact tcaattttcc ctacatttca aaaagcatcg gcgatttctg gcgccgctgg
    21901 cacatttcac tctcaaactg gttccgtgat tttgtttttt atccgctcga acgccgccgc
    21961 ctgaaatggc tcggccagcc gctcaacatt ctcatcgtct ttgcgctgac cggcctctgg
    22021 cacggattaa ctcgcaactt cctcatctgg ggtctgattc atggcatggc attgatcttc
    22081 gaagccacca cccttggaaa aaaactgcgc actgcctggg cgcctgttca acatttgtat
    22141 gcgttgggtg tgatcttagt aggctgggta ttcttccgtt ccccttcgcc tgacttcgcc
    22201 tttgattttc tgcgccgtct tgcaggcaat atgagtggaa tacaagccct cccctttgaa
    22261 acgaccagcc ccctgccatt cattgaaccg accttccttc tggcgctcgc tgcaggcatt
    22321 atattttcgc tcccagttgg aggctggctg caagccaaag ttgacatcct caccagcaca
    22381 agacctgcaa caagaacccc ttttcaattg gcgtatgatc tgggtctctt tttcctgttt
    22441 ctggcagcac ttgcatccac agtgagtgcc gcctttcaac caggcattta cggaaccttt
    22501 tagaacgctg cttcaaaggc ctttttcgca gcagcatatc cctgctggat caggtctctg
    22561 gaaacatcgc cgtcataatc aataatgcgc tcggcaggga gaaaatcatc gggcgcggca
    22621 atgatcactt ttacttcgcg atatttgtac ggcccagcac cttcagcacg ttcacgcact
    22681 gccagatcat tgaaggcttt gatcaaccgc aaacgttcgc ggaatgaagc cagcagcatc
    22741 caatccaggg tccaggtgat ggcatcgcca aaagattcag ggagcttgcg tgccgtgtat
    22801 ggaatggggt caccgctctg ccaccacggt gtcatcaaaa ccactacgat ctcggcaggg
    22861 acatcgatcg gaacatcgcg catggcatcc aatgcaacgc cgatgggggt gtttgccacc
    22921 agtgcgccat cccaataatg ggcgttggtt tcggggtcaa aggtccacgg gtagacgagt
    22981 ggaatcgaac aggaagcaag tattcggtcg gcagtgatcg aggtcactac atcctgccgc
    23041 gcctctccgg tgtcgcgtcg ctggatcgcg cgattgctga acatcatccg ctcccccgtt
    23101 tgcacattgg tggcggcgat caacagggtc ttttggctgc tggcgatctc acgtggatcg
    23161 aatttgaaat gagtgtgcag ggtcttgcgc agcggacctg tatcgagcaa gttgatccag
    23221 cggcccacca aacgctcgcc ccaccatttg gatgtgatcg gcaggggcgg ccaaaattct
    23281 ttcggcatgg gtgaggtggc atcctgtggt gagacttgtg gaaggttggc atccatgatc
    23341 gttccgaaga ttttgtgtgc cacccaacga gccagaccgc gcattcccgg gggcaggccc
    23401 tggatatctt tttcttcgag caattcccaa acacgcacca attcatcggc aggcattccc
    23461 tgtgcaatgg ccgcaccatt caccgcaccg atggaggtgc ccacgatcac ctgcggatcc
    23521 cattgggcgc tatgcgctga atccactttt ggcttgccag attccatcaa gtacttgtaa
    23581 acccctgcat gaaatgcacc acgtccaccg ccgccagaaa gcaccaatgc ccgtactctg
    23641 tccataattc tgctcctttg ttgtattgtt tatagggcaa atcaagacgt atgtcaattg
    23701 cgagttttgg gcggggttac acctcaatgt aaatcaatca ccacaaaccc gcatggcaac
    23761 tttgggggag cggctggctg ttctcacagc cccatcatta atgctatact cccctgcaac
    23821 aaacgactac gcagaaatat cctctacagt ttttacccgc aaccttcttt tggagagagg
    23881 aaatcgaaat gaaaaaaatg attcgtccgt tcataagttc catacttggc ctgttgcttc
    23941 tggcatcctg ctcatttggc agcagtgaca cgcaggccgc cgtggcggcg caagccacat
    24001 ctgcattgcc cgctgtggta acgcttgaac tcacggtaca ggccgacacc tcggtcacct
    24061 acaatacggt tggacaggtc atcaaattaa cgcaaaatgt cagaaacagc ggctcggttt
    24121 ctgcgcccgg tccgatgatc gtcaccggtg ccatctgccc tgatatgacc accatcggca
    24181 acctcgatgc caatttcgac gcgaatgaaa cgatcacctg caattcctcc tacaccatca
    24241 cccaggccga cctcgataag ggcagtgtca cgatcactac catggcaacg gtgaacaatg
    24301 ttaattccaa ccaggtcaca accagcattg ccaccgtacc tcctgtggtt ttaaagctga
    24361 ccaaaaccgc cgcacccacc acatacagcc aggctgggca aaccattact tatagttatg
    24421 tcatcaccaa tgttggagca gcgactttgg gtcccgccca atttacagta acagacgccg
    24481 cctttgccgc tccattcaac tgcggagaag cgaccgcaac cctcgcttcc aacgcgactg
    24541 tgacctgcac agccacttat acgatcaccc aggctgacat gggagtcgca tccatttcaa
    24601 ccaatgcaac cgcaacgggc ggcggcgccc cagtttcgca ggctgtgggg gccaccgtca
    24661 ccaaaagcga tgccgcacag cccagcggcg gacaaagaac tcataccgtc gttgaaggtg
    24721 aatggttgtg gcagatcgcc cgctgttacg gcgtggaccc caacaaactg gcagccgaca
    24781 acaaagataa aattcctgac cccagcaaga tcaagcccgg ggtgattctg accgtgaata
    24841 atcctggtgg ttattccaaa ttctatggtc cgccttgtat cacgtttgta tcccataccc
    24901 ttcaggcagg agatacctgg gccaccctcg gacaaaaata cagcgccgat gccactgtgc
    24961 tccaattagc caaccccgat accagcttga gtgcggcagg aacggtcatc aaactgcgca
    25021 ttccaaatta caccatgggc acagtcacca ccccgcccgc gacacaaagc aaaaccctct
    25081 cagtaactgt cgccgccaac ccaaccgcct acaaccagac agggcaggtc atcaccttta
    25141 cttataccgt caaaaacagt ggcaccgaaa atttggggcc tgctcaattt acagtcagcg
    25201 gcggattgct tggctccact ccattgaatt gcggcgctgc ttcttcaacc ctcgccgccg
    25261 gtgcaacact gacttgtacc tccacttaca ccgtcactca ggaagacatg accgttaatt
    25321 cgatctcgca ccaagtcacc gcttcaggcg gcggagcggg accctcacaa gcggccagtg
    25381 tcaccgtcaa caaatccacc gcactcctga ccttgacgat cgcagcaaat cctctcacgt
    25441 acacacaggc tggtcaaatt atcaccttca cctacgccat taagaacagc ggcagcacaa
    25501 acctcggccc cgctcaattc accatcagcg gcggattgca cggcgcaacc ccgatcaact
    25561 gcggagatgc cgcagctacc atcctgcccg gcggcacgct cacctgtacc gcctcctaca
    25621 ccatcaccga agctgacctt gccgttccaa cgctctcgca tttggtcatt gccgcaggcg
    25681 gaggcgcagc gccttcacaa cctgccagca ttacgctgac aaagcagtaa caaaagcgtg
    25741 ttgcacacat aaaaaaatcg gatgtcattt cgacatccga ttttcattta ctttcccagc
    25801 cttgcccgtt cttccacaga tacgatctca tccaacttct tgaacagcgg acctggttga
    25861 ttgagctttg caccgggctt caagtcgctg ggtttccatt gcatcccttc aacaggcttg
    25921 tagcgcaaaa cggtgtgtgt acccaatgag tcgcttacct cctgggtgta ttgctcgcca
    25981 aaaagcggag tttcgtaatt gaagaagccg tgcagctttt cactggtgaa cggcagaaac
    26041 ggcgcaaaaa tgaccttgag cgagtcaatc gctttgagcg cagtgtagat ggtctttcct
    26101 gcttcgacct tgtccacttt gatggcggac catggcgcgt tgacatcgag atattgattg
    26161 accacggtgg caagtttcat cgcctcacca acggctgagc gcaggtgtac ggcttcgagt
    26221 tccgtgccca cagtgttgaa tccgttttcg atggcggcga gcagttcgag gtccgcgggg
    26281 cgaagcgtgt tcaaatccac atcaggcaca tggccttccc aattcttgta acaaaaagcc
    26341 agcacacgat ttgcaaggtt gccccatgtg gcgaccagtt cattgttgtt gcgagccaca
    26401 aactcagccc aatcccaatc actgtccttc gtttcgggca tgttgatggt caggtaatag
    26461 cggatggcat cagggtcgaa gcgtgtgagc gcatcacgtc cccacacagc ccaactgcgt
    26521 gaaccgctga tcttcttgcc ttcgagattc atgaattgat tggcaggcac atcgtacggc
    26581 aaaatgagcg gaacttcctc acccgcaaac aactccatga acgcactgcc aacgcccatc
    26641 aattcagcgg gccagaggga tgtgtggaag aagatgttgt ccttgccgat gaaatggaat
    26701 tgcttcgcgt ttggattcgt ccaccaatcg cgccacgctt cgccattacc cgatacctga
    26761 ctccactcaa tgggagcaga aagatacccg atcacagcct cgaaccacac gtagagcttc
    26821 ttggtatccc accccgcttc atccaccggg acagggatgc cccaatcgag gtcacgggtg
    26881 atggcgcgcg gcttaagccc ttcggcctcc accttgcgca gcgactcgcc caacacggta
    26941 tcgcgcatgt gatcggcacg gtctttgaga tatttgacaa catcaggctc aagttttgaa
    27001 aggtcaatgt aaaaatgctc tgtatcgcgc agttcagggg atgagttgtc accctcaacc
    27061 ttcgatttcg ggttgaccag tttcatgggg tcaagcacat ttccgcaggt atcgcattgg
    27121 tcagagcgtg cgccttccgt accgcagatg taacaggtgc cttcaatgta acgatcgggc
    27181 aggaagcgat tcaatccagg agaaaaccat tgcggctcag tcttcttgaa caggtagcca
    27241 ttcttttgca gtgcaagaaa aatggtctgc gaaaccttga aatgattctc actgtgtgtg
    27301 gtcgtgtaca ggtcatacga aatgccgatc ttctggaaca ggtcaatgaa cccttcgtga
    27361 taggatttgt acacttcttc cacgggcttg ccgagtttat cggcgctcat ggtgacaggc
    27421 gtgccatgcg agtcggtgcc cgtgatcatc aacaccttgt tacccttcaa acgatggtaa
    27481 cgtgcggcaa tatcaccggg caggtgtgac cctgtcaggt tccctacatg gatttcagca
    27541 ttggcatacg gccaggcaac tgcgatcaag atattttcag gcataagaga ctccacaagg
    27601 aattggatga ttggtcagca aggatgggat tgtgagatgg aaacatccca tatggtttcc
    27661 taatttgcaa taaaaaaggc cgtccgcgaa ttggacagcc cgttttcgtt caggtataca
    27721 gacctaacgt gctttccact cgcggcaaaa catctccatt tgcattcgca tggccatggt
    27781 cgttgtcacc attgggaatt cgggtctgaa tttcatgagc gtattttagg ccgtggaaga
    27841 tggtttgtca atgggtggtt tttgtttcat gcgtgaggcc aaccacagcg acccaagcac
    27901 gatgagtgtt gtgcccaagc tccacagatc cttgtcggtg cctgcctgct gcaccagcgc
    27961 aaacagcgag taaagtacca gcacgatcaa aaacaggaac gcatatatga aattgcgcga
    28021 tttctctacc aacacaccga tgatccacac aatgggaatg aggtatggaa tttggtcgta
    28081 agcccaaaga tagatcgtgc tgacgaaact gatcggaatg atgagattga acgcctccca
    28141 tgccgaccat ttggctggcg cagccccctg tgggtgattc ttccaaagga agaatccagc
    28201 cgccccaagc aggagcaggc tcaaggcggc gccaagaagc gtccagcacg gtgaagtccc
    28261 gccacaggca aggtatgcaa acgcccacac attcgagtgg actccctgtg tccgatccat
    28321 tacggcttcg ctcgcaccgc ggaatttgac cacccacatc gggtcttgaa tcatcccgat
    28381 caacaacagt gcaattccac cgatgaccat cccgtaaatg gctttccagt cgcgtcgcgc
    28441 caaaaaccag atacccgcca aaaaaagaat gggaacaccc tgcggaggtt taagcacggt
    28501 caacgaaagg acaattcccg ccaggaggga tttatctttt tcgagcagca aaatcgccgc
    28561 caggagtgcc agtaaggcaa tcgcaccgac cgaacccgca tgcagggtga gatacattgg
    28621 cccaaagaaa aacattgctg caaaaacagg aaccaacagc agacgctggg cagattcctg
    28681 ccaatgcgca agcagcaaaa agatcgtcgt tgcgacgatc aacaaagtaa tgacctgcca
    28741 tgtgatgtag gcggcaggca gggaaagaaa acccagtgga atgaaaaaga tcgccaacgg
    28801 caggggatac gggaaaattc gattgggctg ccatttgatc ccgtacgttt catgccccgc
    28861 cagatattgt ttttcgtcgt agggattttc gccttcgatc agcattcgcc ctgaaagcca
    28921 aaagaaggtg aaattggaac tttgaaaatc aaaggtgttg taactgagcc gtgcgcgcag
    28981 aaagatcatc cccccgatca aaaccagtgt aacgataaag ccaagcgttt tttgaatgga
    29041 tgagttcatt ttcttcgcag cgcagacttc attctgctgg tatcaaaatg cggactgaag
    29101 tccgcattcc aacatgcctc acttgagcaa atccttcaat ttggcaaacg gcgaatcaga
    29161 cagatcttca tgatggccgc agtctttttc attgaggttc tggccgcatt cggtacacag
    29221 acctttgcaa tcgggcttgc aaattgggct gatgggcact tcgagcaggg catactcgcg
    29281 taacaattca ccgagatcaa tatgggcatc ctcgggcagg atcagcgcag attcggtttc
    29341 ggagcgtttg tcgaaggcgt acaactcggt gaatgaccaa tcgagttcat gatcgaaatc
    29401 gctaagacag cgcacacacc ccagcgtggt ttcagcggaa aaatccgcct gtaccacaag
    29461 cccctgcggc gttttgccta cattgacaat gccttcaaag ttgcgcagtt cgaggtcatc
    29521 accaaggacg atcttgttga actcgaaggg aaagtcatag ttattgccga cttcttcata
    29581 aatgataaag ccgacgttaa tgcggaaggg tttcttggga ctgggcatga gggaaaggat
    29641 gaattatgaa ggatgaaaga tgaaaagaaa aaagaaagaa gaagcaactg aaggaaacca
    29701 ctttctactt tctattcact gctctctgtt ctctaactta cacttttcta tccacaatat
    29761 attttgcaag gttttcgagc gcttcgcgtt cggcgcaatt ctcaaaggtg gtcaaaatgg
    29821 tgagggcgcg gtcaacgtgg cgttcggctt cgcgcatggc gaggcgtgaa gaatcgctgg
    29881 cacggatatt atccacaagg cgggtcatgt tctcgttatt ggtccagccg ccattgggca
    29941 gcgagaggac atccgcatca tctggattgg cttcagcgta gtaaatggct gggagtgtca
    30001 ccagcccatt gtgaaggtct gagccgaggg gtttccccac ggcagcctgt tccccagtga
    30061 aatcgagaat gtcatccacg atctgaaaag ccatgccaac ttgatagcca aaatcgcgca
    30121 tggattcgat gatgccgtca tccacagggc tgaccatggc cgaggcgcgg gaggtcattt
    30181 cgaacagcga tgcggtcttg gcataaatgc gtttgtaata attctcgcgg ttgatcaacc
    30241 cgcgtgaggt gaacatttgc gtcaactcac cgttgacaat gatcgccagc gtttcggaaa
    30301 ataatttcat caacgccaag tgatcggctt cggcggcaag tttggcggcg cgtgcaaaga
    30361 ggaaatcgcc tgtcaataca gtggcgggcg gtgaccagcg cgcattcaag gtgggcatgc
    30421 ctctgcggag caaagcgccg tcgatcaggt catcgtggac gagggtggca gtgtgcaaca
    30481 attcaacggc agcgccgagg gtgacgagtt tgtcgagggg cgcgttcagc atgtttccaa
    30541 cgagcaggcc aagggtaggt cggactcgtt ttccacctgc cacaagcaaa tgctccaatg
    30601 cggcacgcaa gtcaggatgg ctttcatccg cctgcatccg cattcgttct tcaacgagtt
    30661 tgatttcctg tgttactggt gagagaaaag ttactgcgtt caaatcagtc tccccatgca
    30721 aagagccgtg ccgcgttggc ggttgtgata ctggcgatct ctgcagggct ttttaaatgg
    30781 atttctgcaa ttttatcagc aatatgacga acataggcag gttcattacg ttcgccgcga
    30841 tgaggtaccg gggagagaaa aggcgagtcg gtttcgatga ggattctgtc aaggggaatt
    30901 tgtccaataa tttcgcgctt tttgtccgca gttttatagg tcaccgggcc tgtgattccg
    30961 aggaaaaagt tcaggtcgag ggctttttgg gcggtctcaa gatcgccatt aaaagaatgg
    31021 agtacgccag ggcgttcttt gagtgtgccg cttaaaccac tcgtccactc gctcaaaatc
    31081 gcaagcaggt caaccgaggc atcgcccgtc caggcgtccc cttcttcgcg catgtgaatg
    31141 accacaggct tccccacttc ctgcgcgaat tgcagctgcc gcttgagcac ctcgcgttgg
    31201 atcgcccgtt tttccatttc cttcacccag taatagtcaa ttccgatctc accgatggca
    31261 accactttgg gatggcgggc aaggtctttc actttttcga aggttttttc tgtgaattca
    31321 tccaaatcag tgggatgaaa cccgaccgcc gcatacacgc tgggattgga ttctgccaac
    31381 ttcaccgcct ctttgctgga cctgtggtgg agtccgggca cgagcagccg aatcagcccc
    31441 gcatcagtcg cacgttgaat gacttccgcg cggtcggggt tgaatttatg ataatcgagg
    31501 tggcagtgtg tgtcggtgag ttgcatgagg ggatttcagg tttcaggttg aaggttacag
    31561 gttttgctgc cttcatgatg acgtgttgga gggtgtcaat ctttcaggat tgtacccaaa
    31621 aaccgccgcc gattttctgg cgacggtctt ttttgctgac gggttgaaat gattataccg
    31681 tgtggaattt atgacgcgtt cggcagaact tgcagccccg ccgctccgct tccacaatcg
    31741 cctgatcgct cgaccagaag aaattccgct cgccgatggt ttccaccaac ccaccgcgct
    31801 ccatcatttg cctggcattg tcgtgcagac ctgcgaacat gagtgtaccg ccttgtttat
    31861 gcagccgctc atgcaaacgg tgaatggctt caatgcctgc tgtatcgatc agcgacacgc
    31921 cgcgcattga aagaatgagg gcatgagtct ccttcaggtt tgaaaaagcc tcgttgaatt
    31981 gacccgtcgc cgcaaaaaag agcggacccg tgagaaaggc cacgcgcaca tgcctgcaac
    32041 gcccatcggt tttaataccc ttttcttgca gacgtttaat atccacatcc tgaacagtga
    32101 tgtcaatgct ggcgatcttg ttcaggaaga ccgccccagc gaggaaggaa ccaatcagga
    32161 tggcctgggt caggtccagc acaatggtgg cgaacatggt gatggtgaag gcgattatgt
    32221 ccgttttgaa gcgcctgcca aagatgaatt tgatcgcatc ccattcgttc atgcgcacgg
    32281 cagtgaccat caacacacca gccaaagccg caagcggaat ccgctccatg aatacagtga
    32341 gcaggaacat cgatagcaaa attccaacag cgtggatcac actcaccaga cgagtctgtc
    32401 cgcctgattt gatgcccaca ctcgaacgcg cgatcgccgc cgtggcgggc accccgccaa
    32461 agaacgggat caccatattg ccgatgccct gcgcaataag ttcctgattt gcctgcaggc
    32521 gcacacccgt catattcgag ccgaccgcac cgcacaacaa cgattcaact gcgcccagcg
    32581 ctgtgatcgt caacgtgggg gcaatgaaat cagaaaggtt tccccaggga atactggcaa
    32641 aggataaacg ctgttcgagg aacaaggttt gcggaatggc gccgatcatc gagacgggcc
    32701 agctcaatat ccaattaagc agcgtggcga ggatgatgcc cagcaacgac gcagggaaac
    32761 gcgcattcca tctggcaggc cagaacaaca tcgccccgat cacaaccagg gcaagacaaa
    32821 cagaatgcca atcaaatgaa aatggcgtgg tgaaatatcc aatgaacttt tgcgcggcgg
    32881 tttcagcagc cgcggttttg attccaaaaa aattatcgat ctggccgata aagatgatga
    32941 gcgcaatacc ggaggtgaat ccgctgatga ctggtgcagg gatgaacgca atgaatcggc
    33001 ccaaacgcaa aatgccgatc aataaaagca aaaagcccga aaacaacccc gccacccaaa
    33061 taccttccaa gccataacgc gtaacgagca cgatcaacac cgcagacatc gcccccgtcg
    33121 gtccgctgat ctggtatggt gcgccgccga gcagccccat gatgaaacct gcaagaacgg
    33181 cagtgaccag tcccgccgct gcactggccc ccgaagcaac gccaaatgcc agcgccaatg
    33241 gaagcgcaac tgccgcaacg gtcaaaccag caagcaggtc ttgttgaaat ttggcaggcg
    33301 aataattcgc aaactcatct cgatacaatc gcacaaggct tcttcgcggc atggttattt
    33361 ctccaatgaa atcttatctc caactgctgt tgtttgtaat gggatcttct gccgccgcat
    33421 tttcaggcga ctcggcaatt tcctccgcac aaaatgcgca atcacgcctc tctgcttcaa
    33481 cgatggcctg atcgctggac caaaagaaat tttcagcgcc gatggcttcc accaaaccat
    33541 cacgctccat catcgcacgc gcattgtcat ggatgcctgc aaacatgagg gtgccgccct
    33601 gtttacgcag tctttcgtgc agacggtgga tctcctccaa gccttctgta tcgatcagcg
    33661 gaacaccgcg catggacagg atcaaagcat gggtgtcttt caagttggcg aaggcttctt
    33721 caaattgacc tgtggccgcg aagaacaagg ggccagtgag aaacgccaca cggatatgtt
    33781 tgcatttgcc cgccgtttca atgccgcgct gcttcaacct gtcaacatcc acatcctgca
    33841 ggctgatatc aatgctggcg atcttgttga gaaaaacggc gcccgcaagg aaggttccga
    33901 gcaaaatcgc atcggtgagg ctgaggacaa ccgtggcgat catggcaatg ctgaaggcga
    33961 tcatatcggt tttgaaacgc ctggcaaaga tgcgtttgat ggaatcccac tcattggtgc
    34021 ggatggcaac caccataagc aaccccgcca aagccgccag cggaatctgc tcgagaaatg
    34081 gggcgaagag gaacatgacg atcaacaaaa caagggaatg aatgatgctg accaaacgag
    34141 tccgtgcgcc tgattggatc gccaccctgg tgcggatcat ggctgaggtg gtgggaatgg
    34201 cgccgaagaa aggcagggtg atattgccca atccctgccc tataagctgc tgattcacct
    34261 gcaggcggat acccgtcata ttcgagccga ccgcaccgca caggaaagcc tcaatggagc
    34321 caagcgccgt gagggtgagg gcgggagcaa tgaaatcgga aagattcgtc cacgggactt
    34381 tggtgaggtc gagatgttgt tcgaggatca aggttcgggg gatggactga atggttgtga
    34441 ccgaccagcc cagggtttga ttaagaagcg tggcaaggat gagtcctgcc aatgtggagg
    34501 gaaaatataa attccatttt ttcggccaga gcagcatggt cacgatgacg acaagcccca
    34561 acaggatggc ctgtgggttg atgatgaagt cttcgttgaa atatccaatc aatttttgcg
    34621 cggcggtttc ggtagtgggc gtgtggatgc caagcagatt gtccatttgg cggatgataa
    34681 taataagagc aatgccgagg gtgaagccgc ggataacggg agcaggaatg aatgcaatgt
    34741 aacgtccgag tttaagcaga ccgatggtaa gcatgagcaa cccagagaaa agactggcaa
    34801 accacatgcc ttcgagtccg tagcggttga cgagcacgat caacaccaca ctggtgacag
    34861 cctttgcacc gctgacctgg tagggtgccc cgcccaaagc gccgatgaca agcccgccga
    34921 taatggacgt gatcagccca gccgctgccc ccgcgcccga acccgcccca aaggccagcg
    34981 ccaacggcaa agccacagcc gcaacagaca ggcctgctat taaatccttt tgaaaaacgg
    35041 caaatgaata ccctgaaaac tcgtctttat atagacgagc gagacttctt cgaggcatgg
    35101 attgttctcc gaaacgaaaa tttgtttaca aaggctcacg ccgaggcaat tgctcggcac
    35161 aggtgctccc ccaaaaggcg tcctctgttg attggcggtt attttatcac tgttgatttt
    35221 ttttggatgg tcgtaacgaa gaaaatactt cgcccgctat ttttttgatt taaatcgcag
    35281 tatactgaaa aagcaccgat gtcgtcctat ttactggttt ggagaatttt ttatggccgc
    35341 gcacatccca tcctcgatgc tccttgaact aggccaggcg ttctcatcca tgcctgagct
    35401 tgaccccgta ttactctcca gtctttcaca aattcagaat attgtcgcca gtgagggggc
    35461 agccctctgg ctgctcaacg aggccgaagt ggatatcatc tgcacccatg ccaccggggc
    35521 ggatgcctcc aagatcatgg gcagtgtgat gcgtgcccga aaattcgttg cagcgtatcg
    35581 cacctccacg ggcaggctga cacaaacaga ggacccgctc caatccaagt ggatggattc
    35641 aagagcctat cgcacctact tcaacatgtc cgtccgcaat atgatcggga ttccgctcgt
    35701 ctcccgcgga aaactgcttg gcgagatcaa tatcatcaac aaggctggcg agaccgcctt
    35761 cacccacaac gactacgact tcatacatga ggtcactcaa cacattgcgc tcgccattca
    35821 aaacacacaa ctttatgaac gaaacaaccg caccactggg cggcagaaat tgctcaacca
    35881 gatcagccgc cacctgcacc agaccctgga tattgacgaa ctcatccccc gcatttttat
    35941 cgaagtaaat aaagccatca acgctgaagc ccaatccatc tggctggtgg atgaggaagc
    36001 gggcgtcatt cgatgccgct ttgccagggg cgccagcgcc gaaacgctca aaggattgtc
    36061 tgtgccgctg aatgccccca gcattgtcgg caccagcgtc tccaaacagg aatccatcat
    36121 catcaaagat gcgcagaatg acccgcgcca tgcccgttct gtggacgaac gaacgggctt
    36181 tgtcacccgc tcgttgatga ccgtgccgct ggttctggaa gataaatcca tcggcgcgat
    36241 tcaagccgtg aacaaaagag atggtcagct tttcacaaga gacgaccttg acctgttccg
    36301 ctccattgcc gatagtgcgg cattggcagt caacaacgcc caactcgtcg cagacctgca
    36361 aaccagttac gacctcacgc tggatgcgct ttccgccgcg ttggatctgc gcgaccgcga
    36421 aaccgaggga cattcacgcc gcgtggttga atacaccacc tgccttgcac aacaaatcgg
    36481 tctggataag gagagcatca agaacatccg tcgcggggca ctcatccatg atatcggcaa
    36541 gatcggcgtc cccgatgcgg tgttgcacaa accaggctcc ctcgatgaaa cggagcgcaa
    36601 catcatcaac cttcatccgt tggcaggcta caacatgctg gcagggattc cctacctgcg
    36661 cgaggaaatt cagatcgtga tctgccatca ggaaaaatgg gacggcacgg gatatccaca
    36721 cggcctgcgc ggagaggaga ttcccatcgg tgcgcggtta tttgccattg cagatacctt
    36781 cgacgcactc acctccgatc gtccctaccg actgggatgt tcctacgaaa ctgcgcgagg
    36841 gatcatcatc gaagaatcag gcaggcagtt cgatccacgc gccgtggagg ccttcctcgc
    36901 cattcccgaa cacgagtgga tgcatatccg cgcaaaggtg atggaagaga tcgcccatag
    36961 gcgcagcctg caaaagcgcg ttttttgaaa acagggagtg tctaaaagaa tgggttttgg
    37021 ttgccacgaa gtcacaaaga ctcgaaggca caaaggattc ttggtggttt tgtggcttgg
    37081 cgtctttgtg tttaccccaa agacttagcc actcccac
//
LOCUS       DAOQLC010000028        36132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_349_length_36132_cov_22.254012, whole genome shotgun sequence.
ACCESSION   DAOQLC010000028 DAOQLC010000000
VERSION     DAOQLC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 36132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_349_length_36132_cov_22.254012"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..735
                     /locus_tag="PL000_06935"
     CDS             121..735
                     /locus_tag="PL000_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88668.1"
                     /translation="MKYDLQIHHRRSIRLKGYDYTQAGAYFITICSYQREHVFGEVVN
                     GEMKLSAWGEIARAEWFKTAALRDNVELYDEEFVVMPNHIHGIVWLHEVGTRRRRVPT
                     EKFGKPVAGSIPTIIRAYKSAVTYAINGLEKSRGGAIWQRNYYEHVIRNEKELNTIAR
                     YIYYNPFNWQLDRDNANNIRNIPLPETIDDYLLDAEKMMGIKAL"
     gene            complement(766..2328)
                     /locus_tag="PL000_06940"
     CDS             complement(766..2328)
                     /locus_tag="PL000_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HNC88669.1"
                     /translation="MTKIHFSKIQTESKIITARELQIELPSTPVKYYRHGWQSWSLAA
                     WTSLSPLPVQKPAIFHPLQVDAVTVNESRPNGSWLGAVEFADGNVLLLGALATDTTVL
                     LEQDQLTGRSEAGDVEWFMAFGREDVIFAEYAKALEHRFGSIQKNDTPRVWCSWYSLY
                     HAIDEPALHKIFDELTDLPFEVLQVDDGWQMDIGDWTVNEKFPSGMTALAEKIKSTGR
                     RAGLWLAPLIATKTSQLFRTHADWFLRDEHGQLVSAGFNWGQPLYALDTTHPDVTPWL
                     VALMKQVRAWGFDYLKLDFLYGGALKGKRHNDIPREAAYRESLRVMREAMGADAFFLT
                     CGTPILPALGLCDAMRIGPDVGAHWEKHRDSVLFYNFAIPGTRNAIRTVINRLWLDPL
                     VRIDPDVVYFESNENSLTPEQKSLLQDLALICKFKATSDLPQWMTTDEYEHLRAYLNL
                     TPAIKQTGRYTFEIDNRNVDFTPATELPQIPTGLAALWGSLLSWLGDFHFVLRIFKWL
                     DDEALRKRRASL"
     gene            complement(2377..3354)
                     /locus_tag="PL000_06945"
     CDS             complement(2377..3354)
                     /locus_tag="PL000_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC88670.1"
                     /translation="MKNRNLLLYVISGILSAVLLAYLLLNLDWEILRVAFSDLHWGWL
                     GMAFLAYLLNIFLRALRFTNLIYSRPVKWIELVPVSALHNILMYLMPAKTGDVAYIFL
                     AKDRLDLSLTEGTATLLAARYYDFSVVALVLAILLPFSKKDMPVWIFESSVIFCLLIL
                     LGTIGILLFLRFSKPATDNQPVGEKTLITRIRAAWTKFIAGLREIQNHGAHGRVALLT
                     AGIWLCVYSNFYFATRSMGLPVAFYHIAIISIVMIPLTLLPLQGFANIGTHEVGWTSV
                     LVAFGYPYKTALAIAAGSHLVLLFSVLISGGVAFFGAQIISKSIKHEGN"
     gene            complement(3359..4177)
                     /locus_tag="PL000_06950"
     CDS             complement(3359..4177)
                     /locus_tag="PL000_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC88671.1"
                     /translation="MKDTQPILIISTGRTGTIFFSRLFADLYPDSASYHERGASRPVQ
                     ILTNLHFSHLFPKSGLKTAWKLLKGNEIETCEAKFHIDANCFLYGLAPLAPELYPNLK
                     VLHIVRDPRSYVTSHLNFSRQKGTSFIANYFVPFWQPNPFLVGELPLSKAIGFTRFEK
                     YCWIWDFKNRVMEGLENTSTPYMRVRFEDLFNTDNPEDLFGRITDFLGLPRVTGIREK
                     FRQPANTSSKTDFPEWPDWTPKQAAQLQALCGARMAKYGYGGESEWEKKVIGNW"
     gene            complement(4174..5094)
                     /locus_tag="PL000_06955"
     CDS             complement(4174..5094)
                     /locus_tag="PL000_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88672.1"
                     /translation="MPTEKKTSPKITVIIPAFNEGEVIGGVIGRIRAVVNTLDPDNEI
                     IVIDDGSKDNTAQSARDAGARVIEHPYNIGNGAAVKTGIRNARGQILVTIDGDGQHDP
                     NDIIKLVNRIGPYDMVVGSRNRESDTAAHRDVANMVFNSLATYVSGRKIEDLTSGFRA
                     IKAHIARQFVYLLPNQFSYPTTITLSVVRAGYSLGYESIRFARREGKTKSKIKPLRDG
                     LRFLMIILKIAVFYAPLKIFVPASIFTFVLGVAYGLMRIFILNAPYGQTSALLMSTAA
                     LIFMVGLVSEQIAQLRFDRSESNTPLHKDE"
     gene            5266..7224
                     /locus_tag="PL000_06960"
     CDS             5266..7224
                     /locus_tag="PL000_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88673.1"
                     /translation="MPKNKSVLIVISIIFWLFLSLYPWQSWLEWTGVLRFLIGLFIYF
                     VPGVLTFMRVSRDGNFSPRIFLGGFVVSVFVTGLLGLLARLLQLNFTFIRWGFALWGA
                     AAVLLFFLMDVKFTFQFEKFTWWEIVSLLLAAGGAIYFASIARPPLIHDDAFTYNALL
                     YYYQHAPALEFQFPVSLDRLEIPRFWIAYWPLVEALISGFSKVDGLIIAGAYLPPALA
                     CFSFLGIYSLGRTLGLPRAAAGIAILAQGFSLMRLTKWNQPGNLFYQRLTEDKVVAAF
                     FISYIFILLAVEYLEKPEVRKLILVGVAGLAMVFTHPIQFGMACMIVGVYGLPKLLDK
                     DIRWKYATMIGILALVVLIPYTFRFGGGEYSQTLSFSLDDVAENDEFARFGVRRVDII
                     EGTNFYGISHYLTQGLPYEVSLVSALLCLFFFWKYKHARYVLAAFLVLGVSMFPYTGW
                     IVGMFTTPFQLWRLTWLMPFGMAFAFLIWAGFEISQRFEFVRRLQWLQSVYYLGMTVA
                     MLGLVVYVRPWAMENLPPNGIDMVDLYSNYVSAARQMNEMDVDAPIIIGGPDAATNSI
                     IPSLTLKFEPLVFRVQSGGEQTQLWKPLIGENLSPSERLTRFQENKIEYLLIRDEPAW
                     VVQFQQSFPENVSLVFKDRRFSLYRITP"
     gene            7361..7873
                     /locus_tag="PL000_06965"
     CDS             7361..7873
                     /locus_tag="PL000_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNC88674.1"
                     /translation="MYEFDKTDIKIVNLLLEDGRMSASEMSRRMGDISERSIRYRVDR
                     MIDEGVIQISAVVSPEALGFTIKADVWLEVESDLIFEVAKKLASFENVTYVACGIGQN
                     DISIQVVAKDTAEIYYFVTEVVRKVQGVRKTTTSIVPIVLKDVYQWRVPERIAGELSD
                     KESTSTVVSK"
     gene            7892..9190
                     /gene="gntE"
                     /locus_tag="PL000_06970"
     CDS             7892..9190
                     /gene="gntE"
                     /locus_tag="PL000_06970"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HNC88675.1"
                     /translation="MFGSKTQALQQRAQKVLPLGVNSNFRFWGDGITPYVDKTKGGYL
                     WDVDGKKYIDYRMAFGPIILGHAYPEVDSKVIEEIQRGVLFAMTGELEVAVAEMIVEM
                     SPAIEMVRFACSGTEATMHAIRVARAYTGRDIIVKFEGNYHGMHDHTLWSTYSPVEAY
                     GNRRSPIAVPASSGIPKSMREFIITLPFNDFEGFERVMRTHGEQIAAVITEPCQGNCA
                     AINPQDGFLQLIRKKTEEYGCVFILDEVKTGFRIANGGAQEYYNINPDMVTYAKALGN
                     GYPIAAFGGKKEIMSIIGHGVAQGGTYTNNKPGVAGAFATLSLLKSKPILKTIEKRGQ
                     RLMDGLKEIFEENEIPAVFTGYPAMFSFSLNVDKVTCQRDWQESDRDLYLKLVEKAVA
                     KGVMPDHDAREPWFLCYEHSDADIEETLNVYAEIVKEVKK"
     gene            9279..10604
                     /locus_tag="PL000_06975"
     CDS             9279..10604
                     /locus_tag="PL000_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC88676.1"
                     /translation="MTKLTSKEIVDLSKEYTFFSWSVQGQANPIPVEKAEGIYFWDTD
                     GKRYIDFSSQLMNTNIGHQHPKVVKAIQDQAAKLCFVHPGNTTDVRAQLGKKLSEVTP
                     GNLKKTFFSLGGAEANENAIKIARFYTGRHKILARYRSYHGATHGSIALTGDYRRNAV
                     EPAMPGGVHFLDPFCYRCPFGQKKESCKRECISHLEEVIRYEGPDKIAAIIMEGVVGS
                     NGLIVPPDDYWPRVREICDKYGILLISDEVMSGWGRTGNWFAVDNWGVTPDIITTAKG
                     ITSGYVPLGAVIVSDKIADYFEDKYLYAGLTYNGHALACAAALATIEVYEEDNLIENA
                     RTMGNYLGECLEDIKSRHPSVGDVRYIGLFTTIELVANRETKEIVAPAVMAEVGKVLR
                     ENGLFTFIMANNMGSMVFVVPPLCITKEQLDEGLALVEKALEVADGKIS"
     gene            10671..12137
                     /locus_tag="PL000_06980"
     CDS             10671..12137
                     /locus_tag="PL000_06980"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HNC88677.1"
                     /translation="MEILNYINGEWVKPSVKDYFDVINPATGQVIAKTPLGTRADVDA
                     AAKAASDALPAWRRTPVNDRVQYLFKLRNLMRENGDEIAKLITNEAGKTFEEAKAEMV
                     RAYENIEVACGMPHMSKGEFVEDIAPGIDEIMIRQPVGVCATIAPFNFPGMIPFWYLP
                     YALATGNTYIVKPSEKVPMTMQFIFKLIEQVGFPKGVVNMVNGAKETVDGILEHPAIR
                     AITFVGSTNVAKYIYSTAAAHGKRVQAQGGAKNPVIILPDADMEMATKIIADSAFGCA
                     GQRCLAVSLAVTVAESKNHFTELICDAAASRTVGYGMDSGVQMGPVINTASQQRVEQL
                     IALGAKEGAGVPVDGRGTVVKGYEAGSFVRPTILSDVKAGSEIARTEIFGPVLSLMHV
                     NTIDDAIQLVNSGQYGNQASLFTTSGNAARRFRYEADAGNIGINIGVAAPMAFFPFSG
                     WKDSFFGDMHGQGTDAVEFFTQKKVVVERWPKEWSRKF"
     gene            12174..13535
                     /locus_tag="PL000_06985"
     CDS             12174..13535
                     /locus_tag="PL000_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC88678.1"
                     /translation="MSVDYEDALDYSSRWIDIIQKNEFPSPDEAHKIIDESKNNFAEH
                     YNRNWLEYRKSVTEAGDWAAVEWGGSGAVFKDVLGREYLDFLGGYGMLDLGWSHPDVV
                     NTVKAQLERSPMPSQELIDPLRGVLAKLLASITPGDLKYSWFAASGTEANEAAMKIAK
                     LYTGKTAFIVGVKAFHGKTMGSLSLMGKSDYRAPMGAMYGGQVYHVPFGDAEAVEKQL
                     EICDKVGIGVAAVMFEPIQGESGAIVPPDDFWPRIRAATKKYGVLLIADEVQTGLGRT
                     GKLWGVEHWNVVPDILTVAKSLGGGVMPVSAVTTTEEIFKPMMYPNPFMHTTTTGGGA
                     LACSAAISAIHVTLRERLWEKAAEKGSYLIPQLEKFAAQYPQIYEKITGKGLLIGMHF
                     QSPEIGYKVASGLFKRGVLVAGTLTSAQTIRIEPPLVVTMQQIDMLLDRLGDVLSEIS
                     KTI"
     gene            13565..14680
                     /locus_tag="PL000_06990"
     CDS             13565..14680
                     /locus_tag="PL000_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88679.1"
                     /translation="MTSEFAVELRDVVKRFITPEGNEMAAVDHVTMQIKNGEFFSMLG
                     SSGCGKTTSLRMIAGFEWPTEGEVFIEGKPMGHTPPFQRRVNTVFQSYALFQHMTIYQ
                     NVAFGLEMEGAKDEEIKKRVGRALDMVQLTGMDRRKPKQLSGGQQQRVALARALVKTP
                     EVLLLDEPLGALDLKLRKEMQLELKALQQQLGITFIYVTHDQEEALTMSDRIAVMSKG
                     KVQQMGTPVEVYERPANKFVADFIGESNFLEGKIRSISQNEATVFIPGLNAELTGMPV
                     SEGLVKGEEVHVSIRPEKVRVAEKNALNQNMFHAKVTNTVYIGADTHIYVEAHGVKLK
                     VLEQNRISRLDPKSFYSVGQEVWLMLMPENTLILKKD"
     gene            14686..15609
                     /locus_tag="PL000_06995"
     CDS             14686..15609
                     /locus_tag="PL000_06995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88680.1"
                     /translation="MLQRLRESKSAQGTLLALPTTVWLFVLLIIPLILTLIVSFGRRS
                     PDGDVIYTFSLENYIRLSGFSTDCPDGQASCFDPLYLGILGRSLSLAFNTTLFVITLA
                     YPLAYFIARAHPKQRNMYLFMVLIPLWTNFVIRVYAWMMLLRKEGAINVIIGWVMNLL
                     NIPFTPLEMLYTPGAVLVGMIYEFLPFMILPIYTSLEKIDNSLYEAAADLGANSVKTF
                     LRVTLPLSMPGVVAGTILVFIPVMGTFIVSDILGGRQVILVGNLIQRQFLDARDPTFG
                     SAASLILMVLTLIVTYFYTRKFGFGEEIVAA"
     gene            15606..16502
                     /locus_tag="PL000_07000"
     CDS             15606..16502
                     /locus_tag="PL000_07000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88681.1"
                     /translation="MNPFRRSPFVIAAATLVYVFLYAPILVLILYSFNASRTNVTFEG
                     FIPSFSDRLEMDGSLVKASPCGPFHWYCDLANNDDVAEAAGNTLTIAFTATIVATIIG
                     TMAALALQRYNFKMKPFSQLSLYIPIVIPEIVMGIGILTLFSQFFGWANEAFNLQGDA
                     RLSLGIVTVTVSHIAFMVPFVTLVVQARLQGFDKSFEEAAMDLGANEWITFRRVTFPM
                     ILPGVLSGALLAFTLSLDDFVITFFTNGPGSTTLPIYVYGLLRRIITPQVNALSTVWI
                     LVVFTAVLLLQILQNRESKQHS"
     gene            16572..17735
                     /locus_tag="PL000_07005"
     CDS             16572..17735
                     /locus_tag="PL000_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC88682.1"
                     /translation="MSRKNLYGLLGVLVIASMILTACGGSAAPANDSGSGSEGPKVTS
                     TGFECPEPSPRMEVTSTELNIFVWTEYFPQDMLDCFELVYGIKLNRDEYSSNEEMYAK
                     ISAGGSSYDLVQPTDYIIGLMIRQQLLQELDHSKLPNMANMDSGWMDQSFDPGNKYTL
                     PYLGGTDAIVVNTATVKTVPTSWADLWNPEYAGRMIFLDDSRAVIGLTLLTLGYDVNT
                     TDPAQLEEAKAKLAELVPNIKVFDSDSPKTALIAGDVDLGMTWTGEAFIANQENPDIQ
                     YVYPSEGPILWQDNWAIPVGASHTDAIYAWLNYTMQGDVFWLTIRDFQYTNPNKAALE
                     YAKANQPDVYKAYADSPITNPPAEAVANGHGIEDVGDATPLFDDIWVEVKGGQ"
     gene            17999..18469
                     /locus_tag="PL000_07010"
     CDS             17999..18469
                     /locus_tag="PL000_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNC88683.1"
                     /translation="MESLIGSGNPSSRLDNIDQYIIDAMRKDGREAFAQIAEKLNVSP
                     GMIRQRYNRLVELGYLKVVAVTNPLMMGIRTMALIGIHTDGHKMLQVAEEISKIDEVV
                     YLVVVSGRYDIVCEVFCRDHEDLLKFLTEKLAQVDGIRETESFIHLKIMKEIYF"
     gene            18788..19996
                     /locus_tag="PL000_07015"
     CDS             18788..19996
                     /locus_tag="PL000_07015"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNC88684.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKPRAWMEQMVLADYNVKRAKEVQKK
                     LGEKKRFPVEFIDASKKSEIVKLAKKYKVDLIMNAVDPIFNEEIFDAAYSAGVMYMDM
                     AMTLSKPHPKDPFNKCGVKLGDYQFAKAKDWKKKKQLALVGIGVEPGMADVFARYAAE
                     HLFDEIEELGIRDGANIVIEGYEFAPNFSIWTTIEECLNPPVIWEEGKGWFTTAPFSE
                     PEVFDFPEIGPVEVVNVEHEEVLLMPRWVKTKRATFKYGLGDQFIGVLKTLHMLGLDN
                     KEKINVKGVQVAPRDVVAACLPDPAHLGDKMSGKTCAGTWVKGTKDGKPRQVYLYQVA
                     DNKACMKAWGCQAVVAQTAFSAVIAMDLLEHGIWDGVGVLGPEAFPPDPFMEKMADYG
                     FPYGIKEMAA"
     gene            20076..21671
                     /locus_tag="PL000_07020"
     CDS             20076..21671
                     /locus_tag="PL000_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC88685.1"
                     /translation="MKNNSGEGNVISAETKKLKRELTLLPLFGLIYFTVCGGAFGAEP
                     MVGWSGPGLALLLFILTPLFFSIPNMLMVREMQSMMPVEGGYYHWLKVAFGPFVGYMG
                     GWMNWVVSWVDVSIYPVLAAYYLGYFIPALREGAVIGGVFVDSTWLSFGVAVVLIWAV
                     SALNIRGARFSGLLTNWLGVVMLIPLIALTVIGFMAWLKSGETIHLPFLPGGAEVNFS
                     NTIGALSTGIFVAMWNFMGWELPAAAGDEIVNPKKTYPLAMVLVLVAAIATYSLPTLA
                     GLYGGAGEDGRYQMWGIEEYESGEGIGPVLDEYGVPAEQAQSWGVDTASSVGWEFPDI
                     AHVVADKLTGQTNGTLALVMGGLVTFSAVLSMIGLFIGNGLGGTRVPFAMAADGMMPK
                     FMVKVHPRYGTPWVAILFCGLIYSIFSLSAFAALVVIDVFLNMLVLMAEFFAMWKLRF
                     TMPHLERKEVPGGYFGLTLVTIAPAMIIGLAIYSQYVEEGMASISWALGAMAVGAILY
                     FPIRKWIKPGVPDVNPFVAGEED"
     gene            21921..23828
                     /locus_tag="PL000_07025"
     CDS             21921..23828
                     /locus_tag="PL000_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNC88686.1"
                     /translation="MPNGYNGKILHVDLTKGSLSVEEPNEAFYRKYLGGSAMGMHYIL
                     RDMPKGADPLGPDNVLTLFTGVATGAAISGQSRLNANAKSPISGGIGDSQSGGFFPAE
                     LKFAGFDGIVVKGKSSKPVYLAIIDGNYELRDAAHLMGKLTGEVDDIIHKEVDPKAEI
                     LQHGIGAENGVLFSSLCSMSNRHNGRTGMGLVMASKNLKAVVVRGTKKVQLANQKALT
                     ELNRIGPKAIPENGDMDGLAKFGTAVVVLFNNTIGTLPTRNYNEGQFEGCEPISGEKM
                     AETVLKERDTCYACVVKCKRVVEIKDGAYKVDPRYGGPEYETLGTFGSYCGIDDLAAV
                     SLANQICNEYAVDSIAAGATIAFAMECYEKGVITKEQTAGLDLKFGNADAMIKALYML
                     VKGEGEFGKTLGMGSERAAKKWGNGAEDNLITVKGAEAPAHMPQAKRSLALIYAVNPF
                     GADHQSSEHDPYYEEGVGDFNLDRLKQIGLGSPQPAYSLTEEKVRFAYETEVFYSMLD
                     SAELCQFVYGPTWTLYDGKDTANMIGSVTGWDITVEELMEVGRRRLDLFRVFNAREGL
                     TRNADKLPKKFFKQLQGTGPTAGFALTHEEVDSAIDTYYKLAGWTNDGVPTRETLKKH
                     DVEWAAEYLPA"
     gene            23938..25215
                     /locus_tag="PL000_07030"
     CDS             23938..25215
                     /locus_tag="PL000_07030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC88687.1"
                     /translation="MNQLYQDNFSHMTPAWSRIFSFVSERAEGAYIYTDDGKKLLDFT
                     SGIGVTNTGHCHPKVVEAIREQAGLFLHAQANIVVHKPMLQLIDELRKIVPPSLDSFF
                     FANSGAEALENAVKIARVATGRPNVIVFNGSFHGRTHATMALTTSKTIYRTGFAPLPS
                     GVYVTPFPYAFALKMTEEQASQYALDRLEYLLASQTAPKETAAILIESVLGEGGYVVP
                     PTSFMKGLREICDKHGIMLIFDEVQSGFGRTGKWFALEHFDVTPDIITAAKGIASGMP
                     LSGVFSRTDIMKKVETGSIGGTYGGNAVACAAGVATIRAMREEKMLENASERGIQLMT
                     GLRKLQEEYPQIGDVRGKGLMIGTEFIVDGTQAKAKPLVKEIIHKAEEKGMLLLSCGT
                     YDNTLRWIPPLNVSSQQINDGLKIFGEALKESV"
     gene            complement(25275..26372)
                     /locus_tag="PL000_07035"
     CDS             complement(25275..26372)
                     /locus_tag="PL000_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC88688.1"
                     /translation="MENTATGTLLDTFNQDKRFRVPAYYTVCFGSLSSGLRFSVFTDR
                     TRALVDDIIDCLDLERYSFITIFKNWATEDFKNARPIDDYPYEYLFKSDSGQMLVHLI
                     AREGFITVDFYYDVNRPDCEKWVVETNQNLRERFSPAKQPKFKVLMQQEGMFGTEDVN
                     TNDFESINLAEYYNDDFLEIDATISDFMAKKESGIILLHGEPGTGKTTYIKHLISRFH
                     DKNFIFVQNDFVRDLLKPSFVSFLLQNKNASLIIEDAEKVVVSRDNASEDSVVSTILQ
                     LTDGLFSDFLNIKIICTFNTDIERIDKALLRKGRMVAKYNFRALSAEKTAALAKKLGH
                     DDVKGSLTLADIFEFDKRDFKDKSKKVIGFV"
     gene            26483..27841
                     /locus_tag="PL000_07040"
     CDS             26483..27841
                     /locus_tag="PL000_07040"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC88689.1"
                     /translation="MTKSLDRSKLETLLKQEEELFHKNHPRSYEMYQRARKSLHGGVP
                     MLWMIRWAGSFPVFVREAKGAHFTDVDGNDYIDFCLGDTGAMTGHAPEATVKAITEQI
                     QKGITLMLPYEDVIWVGEELQRRFKLPYWQFALTATDANRFALRMARLITGRSKVLVF
                     NYCYHGSVDETFITLDEEGTPISRPNNMGPQIDPRETSKVIEFNDIAALETALSARDV
                     AVVLAEPAMTNIGIIHPDPGYHKALRELTRKYGTYLIIDETHTICAGPGGYTASHSLE
                     PDFLTLGKPLAGGVPAAIYGFTEEVSKAFAAKLAMDDADVGGIGGTLSGNALSIAAMK
                     ATLQHVLTDEFYAKATALQEQFTAGVQSVIKEFELPWIVKQLGNRSEYWFRPNPPKNG
                     GEAHAAVDPELDRYMHLYALNRGILMTPFHNMALISPETTQADVDHHTRVFREAVASL
                     FA"
     gene            27923..29650
                     /locus_tag="PL000_07045"
     CDS             27923..29650
                     /locus_tag="PL000_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNC88690.1"
                     /translation="MAEYKSQIWRVNTRTQELKREPIPETWQRLGGRGLIARILVDEV
                     DAKCDPLGADNKLIFTPGLLVGHMLSSTDRISVGGKSPLTGGIKEANAGGRTGYHMAF
                     MGIHALIIEDMPKEDGYWVLHLSLNGAKWERADDLAGLGVYATAPKLLEKYGDKVAIS
                     MIGPGGEMRMKSAGIQNLDKDKIPSRIAARGGLGAVMGSKGLKAIVFDHAGGQKPPIA
                     DAETFKVAQKDYTQSVMNHPQSITYRDYGTQAMPQMTQRFAALPTHNFSRGTFYDVEK
                     ISGETMREFILNRGKPSDPSHACMAGCTIKCSNVFGGEDGKIIVSPLEYETVGLMGTN
                     LDIDSLDSIGRMNWQVNDLGLDTIEVGGALGVAAEAGLMRWGSEEDGLKLIDEIRQGT
                     ELGRVLGDGAVAVGKKYGVERVPAVKGQAMSAYEPRSIKGTGVTYATTPQGADHTCGL
                     TIRAQVNHLDPTQQKDASLNAQLNMAGYDTIGACIFAGFGYAATPDGVVKRLLKSRYG
                     WEDVPDNILQALGRETIKLEREFNKRAGFTKEDDRLPKWMTEEAIPENGSVFDVSEDV
                     LDHIFDGIE"
     gene            complement(29671..30516)
                     /locus_tag="PL000_07050"
     CDS             complement(29671..30516)
                     /locus_tag="PL000_07050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC88691.1"
                     /translation="MNKTIRNIIIVSLFTIGGGWLGIWLNNVTGNTQPPMQSLGVLVW
                     LTTPAFSGFLLRAFGGDGWKDSGFGLNLISGWKWYLLGILVYPLASLLTFGLGALFGI
                     VSAEGFAAQGFNAYLSAAGMIFVGSLMKNFFEEFAWRGYLTPRFEAAKLHPMLNHFIV
                     SLLWMTWHLPYYYYYLDRATLNSAITTSIPVFLLTGYLVMFPTAILFGEIRLLSKSVW
                     PVFILHNIINALSMPLLINGFIEVKGALAPVFTPTNEGILVSLVFGVVGWFLMQYRLK
                     KQVTP"
     gene            complement(30517..31083)
                     /locus_tag="PL000_07055"
     CDS             complement(30517..31083)
                     /locus_tag="PL000_07055"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC88692.1"
                     /translation="MSLKHAILGFLSFAPFTGYDLKKAFDNSVQHFWPANQSQIYRTL
                     AELEENGFVEKEVIAREERLDMKVYHITATGREELHQWLAAPLPERDDREPFLIQVYF
                     GGQLNDTELLKVFQDKLKKIEEQLALYEAVFQMTQNTPSKVSDPRTVFLSMLTLEAGY
                     INTQSIAAWLRSAIARIENKNYTINMGK"
     gene            complement(31193..33385)
                     /locus_tag="PL000_07060"
     CDS             complement(31193..33385)
                     /locus_tag="PL000_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88693.1"
                     /translation="MNKYISEILKIKTRSFNARELVLATLITGVVGAALMAAININRA
                     EMIPLSLLLFIALVLGYFGYLNAARWVSLFTTLAIISSLVYKNNGIRDPAILGLVVVL
                     IAAGLLAGRRGTIIVGALILAEVILFGFLETQGYITNKFSHFNTFADYFSTSLCVVLV
                     TVFQWLVIARLNENIKTAELEIRERETIEKQLRDAETRYRNLVEQIPAVVYISEPGAA
                     GIWHYVSPQIEALTGFTPEEWTLNKDLWYRLIHPDDRERTVEAELSSLNSGQMPRTEY
                     RLQTRSGNYVWVQDQSIRSMENNVLQGFLVDITARKMAEDQLQKRLAELNAVRGVSET
                     LIAQTDLKELIQQTGDQIKVTFNVDNLFISLLNATTSLIHFPYYVEKGQQIQAPSLTF
                     GKGMTSSVLRTKKAFLINTNWAQVSAEYGVIYYDNSPARSSLTVPLMIGEKGIGAISV
                     HDMEKENAFSENDLRLLSTIAANLAVAIENTRLQESLKRELTIQEKLVTELEQKNAEL
                     ERFTYTASHDLKSPLITIRGYLGYLEKDARNGNFDRLQQDIGRISEAAEKMHNLLNDL
                     LELSRVGHIINEPRNISFSEVVEEGLKRVESQLKEKQIQVKIGSDLPQINGDKERLIE
                     VIQNLVENSCKFMSDQPKPIIEIGVEKRDGENIFFVKDNGPGIRQDFHERVFGLFDKL
                     DPTSSGTGIGLALVKRIVEVHGGRIWIDSYGYGSGTTFYFTLTDKSSS"
     gene            complement(33468..33540)
                     /locus_tag="PL000_07065"
     tRNA            complement(33468..33540)
                     /locus_tag="PL000_07065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33505..33507),aa:Thr,seq:cgt)
     gene            33635..34921
                     /locus_tag="PL000_07070"
     CDS             33635..34921
                     /locus_tag="PL000_07070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC88694.1"
                     /translation="MLRIAMLSYHTCPLATLGGKDTGGMNVYVRDLTCELGRMGIHVD
                     VFTRSQDEHVPHVVHELGFGNRVVHIPAGPEEPKSKSDIAKFIPEFVEGIKQFAAEKN
                     IHYDVIHSHYWMSGLAAEALSDAWGGTPIVHMFHTLGEMKNRVARSEDERAGEDRLKG
                     ERQVLGRADRIVVATLAELTQLRFLYRANARKLVVIPPGVDTSHFYPIPADEAKQYIG
                     LTPDSRMVLFVGRIEPLKGVDTLIQAMSCLGVNEIHKPVHLAIIGGEPDVIPEKMSAE
                     MARLQKLCDDLCMGGMVVFLGKRGQDTLPYYYSAAEVLVMPSLYESFGMVALEAMACG
                     TPVIASEVGGLGYLVQDGETGYTIPDSDPEALCNKLSGLLGDAHLRETMGLRAAEYAL
                     DYAWSNIAAQIVDVYKGLVEKRSVGVLLTGSDAVRS"
     gene            34973..35449
                     /locus_tag="PL000_07075"
     CDS             34973..35449
                     /locus_tag="PL000_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88695.1"
                     /translation="MRLLSMRPLYIFLLSSVFVFVAGCSAPQTTVNNVPVSEPTSNDL
                     PPSSVAFVDARFAAEATAIQLLETLDGANWLEGPRVLYVEEMSYEQANQKIGLGEGDF
                     VTWPQGTRVWFVIASGRWQVTQFGPNPEIYEGCLFMVYSVSGANIIAMGDSVCPTN"
     gene            complement(35468..>36132)
                     /locus_tag="PL000_07080"
     CDS             complement(35468..>36132)
                     /locus_tag="PL000_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88696.1"
                     /translation="LSTFVSTPTPVADCPPPLIQTLSNNGNATPAPYPLPTATPAAPV
                     VLPASPTPLLFTDPSIPISERIVHYYFITPAENPPPDGSIVVISYILAPTPSDRTYGP
                     DTATNIRASLENVLRDERNGWTSTNVAIVDVTFNDGHTNVILQGEYFGVGDVTLIAAR
                     MQILMTVFANPFVQTATITLNGDTIGNMGISNSLEAKPAGYIFTRSEIETFQRDHVYG
                     TP"
BASE COUNT         8520 a         8632 c         9830 g         9150 t
ORIGIN      
        1 cgttggtggc cgcgggcaga aaagtattgc gtgtgaagtt gaattcggtg gaggatttga
       61 agaagttgtt gtaaattttt gttggggcac ggcggcgccg tgccccaacc gagttgatgg
      121 atgaaatacg atctccaaat tcatcatcgg cgttcgatcc gtttgaaggg atatgattac
      181 acacaggcag gtgcgtattt catcacgatt tgttcgtatc aacgggagca tgtgtttggt
      241 gaggttgtga acggggaaat gaaattgagc gcatggggcg agatcgcccg cgcggaatgg
      301 ttcaagactg cggcattgcg tgataacgtg gaattgtatg acgaggaatt tgtcgtcatg
      361 cccaaccata ttcatgggat tgtttggttg catgaggtag ggacacggcg ccgccgtgtc
      421 cctacggaga aattcggaaa acccgttgcg ggttcaatac caaccatcat ccgcgcatac
      481 aaatctgcag tgacctatgc cattaacgga ttggaaaaat cgcgcggcgg tgcgatctgg
      541 caaaggaatt attacgagca tgtcatccgc aacgaaaaag aactgaatac catcgccaga
      601 tacatttact acaatccctt caactggcag ttggatcgag acaatgcaaa taacatccgc
      661 aacatcccct tgcccgaaac cattgacgac tatctgctgg acgctgaaaa gatgatgggg
      721 ataaaagcct tataaatgat cgtttttata aaatcggggt gttttctaca aactggctct
      781 gcgtttcctc aacgcctcat catccaacca tttgaaaatc cgcagcacga aatgaaaatc
      841 tcccagccaa ctgagcaggg atccccacaa cgccgccagc ccagtgggga tttggggtaa
      901 ttctgtggcg ggggtgaaat ccacgttacg gttgtcaatt tcgaaggtgt agcgtcccgt
      961 ctgtttgatc gcgggagtca gattcaagta tgcgcgcaga tgctcgtact catcggttgt
     1021 catccactgc ggcaggtctg aagtggcttt gaacttgcag atcagcgcga ggtcttgcag
     1081 cagggatttt tgttcggggg tcagcgaatt ttcattcgat tcaaaataca ccacatcagg
     1141 gtcaatgcgc acgagcggat cgagccacaa gcgattgatc accgtgcgga tggcgttgcg
     1201 cgtgccagga atggcgaagt tgtaaaacag caccgagtcg cggtgttttt cccagtgtgc
     1261 gcccacatcg ggtccgatgc gcatcgcatc gcacaagccg agcgcaggca ggatcggcgt
     1321 gccgcaggtc aggaagaacg cgtccgcgcc catcgcttcg cgcatcacgc gcaggctttc
     1381 gcgatacgcg gcttcgcgcg gtatgtcgtt gtgccgtttg cctttcaacg cgccgccgta
     1441 cagaaagtcc agttttaggt agtcaaagcc ccaggcgcgg acctgcttca tgagcgcaac
     1501 cagccacggg gttacgtcag gatgcgtggt gtcgagcgcg tagagcggct gtccccagtt
     1561 gaatcccgcc gagaccagtt gtccatgctc gtcgcgcaga aaccaatctg catgggtgcg
     1621 aaagagttgg gatgtttttg tcgcgatcaa cggcgcgagc cataaccccg cccgcctgcc
     1681 cgttgatttt attttctccg ccagcgccgt catgcccgac ggaaattttt cgttcaccgt
     1741 ccaatcgccg atgtccatct gccagccgtc atccacttgc agcacttcaa aaggcaggtc
     1801 ggtcaactcg tcgaaaattt tatgcagcgc gggctcatcg atggcgtgat acaggctgta
     1861 ccacgaacac cacacgcgcg gtgtatcatt tttttgaatg gacccgaagc gatgttccaa
     1921 cgctttggca tactcggcaa agatgacatc ttcccgacca aaagccatga accactcgac
     1981 atcgcccgct tcgctccgcc ctgtgagttg atcctgttca aggagcacgg tcgtgtctgt
     2041 ggcaagcgcg cccaacaaaa gcacattgcc atctgcaaat tccaccgcgc ccagccacga
     2101 cccgtttggc cgtgactcat tcaccgtcac cgcatccacc tgcagcggat gaaagatggc
     2161 aggcttctgc acaggcagcg gactcaaact cgtccatgcc gccaacgacc acgattgcca
     2221 gccgtgacga taatacttca caggcgttga aggcagttcg atctgtaact cgcgtgccgt
     2281 gatgattttt gattcagttt ggattttcga gaagtgaatt ttggtcatat taaccttgca
     2341 aaaacaaagg ctacgaaggg gaaatccttt gtgtacttag tttccttcgt gtttaatgct
     2401 tttggaaatt atctgtgcgc caaagaatgc caccccgccg ctgatcaaca ccgaaaacag
     2461 cagcacaagg tgagaaccag ccgcaatcgc cagcgcggtt ttgtaaggat agccaaatgc
     2521 caccagcacc gaagtccagc cgacctcatg cgtgccgatg ttggcaaagc cttgcaaggg
     2581 gagcagggtg agggggatca tcacgatgga aatgatcgca atgtgataaa aggccacagg
     2641 cagccccatg ctgcgcgtgg caaagtagaa attggaataa acacacagcc agattcccgc
     2701 tgtgagcaga gccacccgtc catgtgcgcc gtggttttga atctcgcgca atcccgcgat
     2761 gaattttgtc cacgccgcac ggatgcgggt gatcagcgtt ttttcgccaa ctggctggtt
     2821 gtctgttgca ggtttgctga atctcagaaa gagcaaaatg ccgatggtgc cgagcaaaat
     2881 caacagacag aaaatgaccg atgattcaaa tatccaaacg ggcatatcct tttttgaaaa
     2941 cggcagcaag atcgccaaaa ccaacgccac gaccgaaaag tcatagtaac gcgccgccag
     3001 cagggtcgct gtcccttctg tgagggaaag gtccaatctg tctttcgcga ggaagatata
     3061 tgccacatcg cctgttttgg cgggcatgag atacatcaaa atattgtgca gcgccgaaac
     3121 ggggaccaac tcgatccact tcacagggcg ggaatagatc aaattcgtaa agcgcagcgc
     3181 ccgcaagaaa atgttgagca aatatgccag aaaagccatg cccagccagc cccagtggag
     3241 atcagaaaac gccacacgca aaatttccca atcaaggttg agcagcaaat aagcaagtaa
     3301 aacagcagag agaattccgc tgatgacgta aaggaggaga ttacggtttt tcatccaatt
     3361 accaattacc aattaccttt ttttcccatt cactctcccc gccatagcca tacttcgcca
     3421 tccttgcccc gcacaacgcc tgcaactgcg cagcttgttt cggcgtccaa tcgggccatt
     3481 cgggaaaatc ggttttggat gaggtgttgg ctggttgacg gaatttctcg cggatacccg
     3541 tcacacgcgg caggccgaga aaatctgtga ttctgccgaa tagatcttcg gggttgtcag
     3601 tgttgaacaa gtcttcgaag cgtacacgca tgtagggcgt ggatgtgttt tcgaggcctt
     3661 ccatcacacg gttcttgaaa tcccagatcc agcagtattt ttcgaagcgg gtgaagccaa
     3721 tagctttgct gagcggcagt tcgcccacca aaaacggatt cggctgccag aacgggacga
     3781 aatagttggc gatgaaactc gtcccttttt ggcgggagaa gttcaaatgt gaggtcacgt
     3841 aactgcgcgg atcgcgcacg atgtgcagaa ctttcaggtt tgggtaaagt tcaggcgcga
     3901 gcggggccag cccatacaaa aagcaattcg catcaatgtg gaatttcgct tcgcaggttt
     3961 caatctcgtt tcctttgagc aatttccacg cggttttcag tccgcttttg gggaagagat
     4021 gcgaaaaatg caaattggtg aggatctgga ccggtctcga agcgccgcgt tcatgataag
     4081 aagcagaatc gggatacagg tcggcaaaga ggcgtgagaa aaaaatcgtc cccgtgcgcc
     4141 cagtggagat gatcagaatc ggttgggtat ctttcattcg tctttgtgga ggggggtatt
     4201 cgattcgctg cggtcaaagc gcaattgagc gatctgttca gagaccagcc ccaccatgaa
     4261 gatcagcgcc gcggtggaca tcagcagggc ggaggtctgc ccgtaggggg cgttgaggat
     4321 gaagatgcgc atcaacccgt atgccacgcc gagcacaaaa gtgaagatgc tggcagggac
     4381 gaagatcttc aacggcgcgt agaaaactgc gatcttcaaa atgatcatca aaaagcgcag
     4441 accgtcgcgc agtggcttga tcttgctttt tgtttttccc tcgcgccgtg caaagcggat
     4501 cgattcgtac ccgaggctgt accccgcgcg gacgactgac aatgtaatgg tggtgggata
     4561 cgaaaattga ttcggcaaaa gataaacgaa ttggcgcgca atgtgcgctt tgatcgcgcg
     4621 gaaacctgag gtcaggtctt cgatcttgcg ccccgaaaca tacgtggcga gcgaattgaa
     4681 aaccatgttg gctacatcgc gatgcgcagc cgtgtctgat tcacgattgc ggctgccgac
     4741 gaccatgtcg taggggccga tgcggttcac gagtttgata atgtcattgg ggtcatgctg
     4801 gccgtcaccg tcaatggtga cgaggatctg tccgcgtgca ttgcgaatcc ctgttttgac
     4861 cgcggcgcca ttgccgatgt tgtagggatg ttcgatcact cttgccccgg catcgcgcgc
     4921 actttgggcg gtgttgtcct ttgaaccgtc gtcaatgacg atgatctcgt tgtctgggtc
     4981 cagtgtattc accaccgcgc gaatccgccc gatcacgccg ccgattacct cgccctcatt
     5041 gaaggcaggg atgatgaccg tgatcttcgg tgatgtcttt ttttctgtag gcattcattg
     5101 ctccataaaa gccgattact cggagcgcgg acttcagtcc gcgttgtgtt atcgtggcgg
     5161 actgaagtcc gcattccaat catgatttgc ttccccaaaa agcgcgctgg gataataatc
     5221 gaaacaagta taattggcta ggtatcccca attttaggaa gaatcatgcc caaaaacaag
     5281 tctgttctta ttgtcatctc cattattttc tggttgttcc tctcgttata tccctggcaa
     5341 tcctggctcg aatggacagg cgtcctgcgt tttctgatcg gcctgttcat ttattttgtc
     5401 cctggggtgc ttacatttat gcgtgtcagc cgcgacggaa atttttctcc gcgcattttt
     5461 ctaggcgggt tcgtcgtgtc ggtttttgtg acggggctgc tcggtctgct ggcgcgtttg
     5521 ttgcagctca acttcacctt tatccgctgg ggatttgcgt tgtggggtgc ggctgccgtt
     5581 ctgctatttt tcctgatgga tgtgaaattc acttttcaat ttgaaaaatt cacatggtgg
     5641 gagatcgttt ccctgctttt ggcggcgggt ggcgcaattt actttgccag catcgcccgt
     5701 ccgccgttga tccacgacga cgccttcacc tacaatgccc tgctttatta ctaccagcat
     5761 gcacccgcac ttgaatttca attccctgtt tcactggacc gtttggaaat cccccgcttt
     5821 tggatcgctt attggccgtt ggtcgaagcc ctgatctctg gattcagcaa agtggatgga
     5881 ctcatcatcg cgggggcgta tcttcccccc gctctggcct gcttctcctt cctgggcatt
     5941 tactccctcg ggcggactct cggcctgccc cgcgccgcgg cagggattgc catcctcgca
     6001 caaggcttca gcctgatgcg cctgacgaag tggaatcagc ctggcaacct tttttaccag
     6061 cgccttaccg aagataaggt cgttgcggca tttttcattt catatatttt tattctgctg
     6121 gcagtggaat atctggaaaa gcccgaagta cggaaattga ttctcgtcgg ggtggcgggg
     6181 ttggcgatgg ttttcacgca tcccatccaa tttggcatgg cttgtatgat cgtcggggtt
     6241 tatggcttgc cgaaactgct tgataaagat atccgctgga aatatgcaac gatgatcgga
     6301 atccttgcct tggtggttct gattccgtat accttccgct ttggcggggg cgaatattcg
     6361 cagaccctca gcttctcgct cgacgatgtc gccgagaacg acgaattcgc ccgtttcggt
     6421 gtccgccgcg tggatattat cgaagggacg aatttctacg gcatttcaca ttatctgact
     6481 caaggtctgc cgtatgaggt cagccttgtt tcagctttgc tatgtctttt cttcttttgg
     6541 aaatataaac atgcccgcta tgtactggcg gcatttctcg ttttgggcgt gagcatgttc
     6601 ccgtacacag gctggatcgt cggcatgttc accacgccgt ttcagttgtg gcgcctcacc
     6661 tggctgatgc cgtttggaat ggcgtttgca tttctgatct gggctggttt tgaaatttca
     6721 caaagatttg aattcgtccg ccgtttgcaa tggcttcaat ccgtttatta tttgggtatg
     6781 accgtagcca tgctggggct ggtcgtttac gtccgcccgt gggcgatgga aaatctcccg
     6841 cccaacggca ttgatatggt ggatctgtat tccaattacg tcagcgcggc acggcagatg
     6901 aacgaaatgg atgttgatgc gccgatcatc atcggtgggc ctgatgccgc caccaattcc
     6961 atcattccca gcctgacgtt gaaattcgag ccgctggtct tccgtgtgca atcgggaggg
     7021 gagcagaccc agctctggaa accccttatc ggggagaatc tttccccgtc agaacgcctg
     7081 accagatttc aggaaaacaa gatcgagtat ctgttgatcc gcgatgagcc tgcgtgggtg
     7141 gtccaattcc agcaaagttt ccccgaaaac gtctcccttg ttttcaaaga ccgcaggttt
     7201 agtttgtatc gaattacgcc ctaaacagga acacgggata ttatctcgta ttactaactc
     7261 ggccgtcacc ttgtgtggcg gcttttttat tcccccttga cgattaaaac gccacaaggt
     7321 atattctgcg cgtaactttc ggaaggtata aagtgaatct atgtatgaat tcgacaaaac
     7381 agatataaaa atcgtcaact tgttgcttga agatggccga atgtctgctt ctgagatgtc
     7441 ccgtcgtatg ggtgatatct cagagcggtc aattcggtat cgtgttgacc gcatgatcga
     7501 cgaaggggtt attcagatca gtgcggtggt cagccccgag gcgctgggtt ttaccatcaa
     7561 ggccgatgtc tggctggagg tggaatcgga ccttattttc gaagtggcga agaaattggc
     7621 ctcttttgaa aatgtcacct acgtggcttg cggcatcgga cagaacgaca tcagtattca
     7681 ggtggttgcc aaggacacgg cggaaatcta ttattttgtg actgaagtgg tgcgcaaggt
     7741 gcaaggggtg cgcaagacca ccacgtccat cgtgccgatc gtcctcaaag atgtgtatca
     7801 atggcgggtg cctgaacgga ttgcaggcga attatcggat aaagaatcga catcgaccgt
     7861 ggtatccaaa taaatcagta aaggagagtg tatgtttggt tcgaagacgc aagctttaca
     7921 acagcgcgcg caaaaggttt tacctttggg ggtgaactcc aattttcgtt tttggggtga
     7981 cgggatcacc ccgtatgtgg ataagaccaa gggcggatat ttatgggatg tggacggaaa
     8041 gaaatatatt gattatcgta tggcttttgg gccgatcatc ctcggtcatg cttaccccga
     8101 ggttgattca aaagtaattg aagagattca gcggggagta ttgtttgcca tgacgggcga
     8161 gcttgaggtg gctgtcgcag aaatgatcgt ggaaatgtca cctgcgatcg aaatggtgcg
     8221 ttttgcctgt tcaggtaccg aagccaccat gcacgccatc cgcgttgccc gcgcgtacac
     8281 tgggcgcgat attattgtga aatttgaagg caattatcat ggtatgcacg accatacctt
     8341 gtggtccacc tactcgcctg tggaagcgta tggcaatcgc agaagtccca ttgcagtgcc
     8401 ggcctcttcg ggtattccca aatccatgcg cgaattcatc atcaccctgc cttttaatga
     8461 ttttgaaggt tttgagcgtg tgatgaggac acacggcgag cagatcgccg cggtcatcac
     8521 cgaaccgtgt cagggaaatt gcgctgcgat caatccgcag gatggatttt tacagcttat
     8581 ccgcaaaaag accgaggaat acggttgtgt tttcattctt gatgaagtga agacgggctt
     8641 ccgcattgcg aacggtggtg cgcaggagta ctacaacatc aaccctgaca tggtcaccta
     8701 cgccaaggcg cttggaaatg ggtatcccat tgcggctttt ggcggaaaaa aggagattat
     8761 gtccattatt gggcatggcg tggcgcaagg cggcacgtac acgaacaaca agcctggtgt
     8821 ggctggggcg tttgccactt tgagcctgct caaatccaag ccgattctta agaccatcga
     8881 aaagcgtgga caacgcctga tggacggttt gaaggaaatc ttcgaagaga acgaaatccc
     8941 tgctgttttt acggggtatc ctgccatgtt ctctttctcg ttgaatgtgg acaaggtcac
     9001 ctgtcagcgc gattggcagg aaagcgaccg tgacctgtac ctgaaacttg tggagaaggc
     9061 tgttgccaaa ggcgtcatgc ctgaccacga tgcccgcgaa ccctggttcc tttgctatga
     9121 acattccgat gcagacatag aagaaacctt gaacgtgtac gcggaaatcg tgaaggaagt
     9181 gaaaaaataa ttgtttaaag gtttgaaggt tgaaaagttt gaaggtttcg acttgttaac
     9241 tcttaaacct gtgtacttgt taaacctatg gagaatccat gacaaaacta acatccaaag
     9301 aaatcgttga ccttagcaag gaatacacct ttttctcgtg gtcggtacag ggccaggcca
     9361 atcccatccc cgtggaaaag gcggagggca tttatttttg ggatacggat ggaaaacgct
     9421 atattgattt ttcatcccaa ctcatgaata ccaacatcgg tcatcagcac cccaaagtgg
     9481 tcaaagccat tcaagatcag gcggcgaagt tgtgttttgt gcatccgggg aacaccactg
     9541 atgtccgtgc tcagcttggt aaaaaactct cggaagtgac cccgggaaac ttgaaaaaga
     9601 catttttctc cctcggcggt gcagaggcga atgagaacgc catcaagatc gcacgttttt
     9661 acacgggccg tcataagatt ctggcccgct atcgttcgta tcacggcgcg actcacggct
     9721 ccattgcact gacaggcgat taccgccgca atgccgttga acctgccatg cccggcgggg
     9781 ttcatttcct tgatccgttc tgctatcgct gtccgttcgg gcagaagaag gaatcctgca
     9841 agcgcgagtg tatttcacat ctcgaagagg tgatccgtta cgaaggtccc gacaagatcg
     9901 ccgcgatcat tatggaaggc gtggtcggct cgaatgggct gatcgttccg cctgatgatt
     9961 attggccgcg cgtccgcgag atctgtgata aatacggcat tttgctgatc tcagacgaag
    10021 tgatgagcgg atggggccgc accggtaact ggtttgccgt ggacaattgg ggcgtcactc
    10081 ccgatatcat caccaccgca aaaggcatca ccagcggata tgttccgctc ggcgcagtga
    10141 tcgtttcaga caagatcgcc gattattttg aagataaata tctttacgct ggacttacgt
    10201 ataacggcca tgctttggct tgtgccgcag cgcttgccac tatcgaggtg tacgaagagg
    10261 ataacctcat cgaaaacgca cggacgatgg gtaactacct tggcgaatgt cttgaagata
    10321 tcaaatcacg gcatccctcg gtgggtgatg tgcgttacat tggcctgttc accaccattg
    10381 agttggtcgc caatcgggaa accaaagaga tcgttgctcc tgccgtcatg gccgaggtgg
    10441 ggaaggtact gcgcgaaaac gggcttttca cctttatcat ggcgaacaat atgggcagta
    10501 tggtgtttgt cgttccaccg ttgtgcatca caaaagaaca actggacgaa ggcctggcat
    10561 tggttgaaaa agcgttggaa gttgcggatg gaaaaatcag ttaacaagtt gacaagttgg
    10621 aaggtctgaa cgtttcaacc ttccaacttt caaacgtgaa tggagaactc atggaaatcc
    10681 taaattacat taacggcgaa tgggtcaaac cctcggtcaa agattatttt gatgtcatca
    10741 accctgccac gggacaagta attgcaaaga cgccgcttgg cacaagggca gatgtggatg
    10801 cagcggcaaa ggccgccagc gatgccttgc cagcatggcg cagaaccccg gtcaacgatc
    10861 gggtgcaata cctgttcaaa ctccgcaatc tcatgcgtga aaacggcgat gagattgcca
    10921 agctcatcac caacgaagca ggcaagacct ttgaagaagc caaagctgag atggtgcgcg
    10981 cctacgaaaa cattgaagtc gcctgtggta tgccgcacat gagcaagggc gaatttgttg
    11041 aagatattgc ccccggcatt gatgagatca tgatccgcca gcctgtgggc gtatgtgcca
    11101 ccatcgcgcc atttaatttc ccgggcatga tcccgttctg gtatttgccg tacgcacttg
    11161 ccacgggcaa cacgtacatc gtcaaacctt ctgaaaaagt acccatgacg atgcagttca
    11221 ttttcaaact cattgagcag gttggcttcc cgaagggcgt ggtcaacatg gtcaatggcg
    11281 cgaaggaaac cgtggatggc atattggaac accctgccat tcgcgccatt actttcgtcg
    11341 gctcaaccaa cgtggcgaag tatatttact ccactgctgc cgcacatggc aaacgtgtgc
    11401 aggcgcaagg cggagcgaag aaccctgtca tcattttgcc tgatgcagat atggaaatgg
    11461 cgacgaagat catcgccgat tctgccttcg ggtgtgctgg tcagcgctgt ttggcagttt
    11521 ccctggctgt gacagtggct gagtcaaaaa atcatttcac cgagttgatc tgcgatgccg
    11581 cggcatctcg caccgtcggc tacggcatgg attctggtgt acagatgggc ccggtgatca
    11641 ataccgcgtc acaacaacgc gtggagcaat tgatcgcatt gggtgcaaag gaaggcgctg
    11701 gtgttccggt ggatggacgc gggacggtgg tgaaaggcta cgaggctgga tccttcgtca
    11761 gaccgacgat cctctccgat gttaaagcag gaagcgaaat cgcacggacg gagatcttcg
    11821 gaccggtgct cagtctcatg catgtcaata ccatcgacga tgccatccaa ttggtcaaca
    11881 gcggacagta tgggaatcag gccagcttgt tcaccaccag cggcaatgcg gcacgccgtt
    11941 tccgctatga agcggatgca ggcaacatcg gtattaatat cggtgtggct gctccgatgg
    12001 cgttcttccc gttcagcggc tggaaagatt ctttcttcgg cgatatgcac ggacagggca
    12061 ccgatgcggt ggaattcttc acccaaaaga aggttgtggt cgaacgttgg cccaaggaat
    12121 ggtcgagaaa attttaatgt aactcaaacc taagtttgcg ttacggagga tgtatgtcag
    12181 tagattacga agatgcattg gactattcat ctcgctggat cgatatcatt caaaagaatg
    12241 agtttcccag cccggatgag gcgcacaaga tcattgatga atcaaagaat aattttgccg
    12301 agcactacaa ccgtaactgg ttggaatatc gcaaatcagt gacagaggcc ggggattggg
    12361 ccgctgtgga gtggggcggt tccggggctg tattcaagga tgtcctcggg cgcgagtatc
    12421 tggatttcct cggcggctac ggcatgttgg acttgggctg gagccacccg gatgttgtaa
    12481 atacggtcaa ggcgcagttg gaacgttcgc cgatgccgtc acaggaatta attgatcccc
    12541 tgcgcggtgt gctggcaaaa cttttggctt ccatcacccc cggtgacctg aaatatagct
    12601 ggtttgctgc cagtggcact gaagccaatg aagccgcgat gaagatcgcc aagttgtaca
    12661 caggcaagac ggcattcatc gttggtgtga aggcatttca cgggaagacg atgggctcac
    12721 tttccttgat gggtaaatcc gactaccgtg cgccgatggg agcaatgtat ggcggccagg
    12781 tttatcacgt tccatttggc gatgcggagg ccgtggaaaa acaactggag atttgcgaca
    12841 aggtcggtat cggtgtggcg gcggtgatgt ttgaaccgat ccagggtgag tcgggggcga
    12901 ttgtgcctcc cgatgatttt tggccgcgca ttcgcgccgc tacaaagaaa tatggtgtgt
    12961 tattgatcgc cgacgaagtg cagacgggtc ttggccgcac cgggaaattg tggggcgtgg
    13021 agcattggaa cgtcgtcccc gatattttga cagtggcgaa gtcgcttggc ggcggtgtga
    13081 tgcccgtcag cgcggtgacg accacagaag aaatattcaa gcccatgatg tatccgaatc
    13141 cttttatgca taccacaaca acgggcggcg gcgctttggc ttgttctgcg gcaatttcgg
    13201 ccattcatgt aaccctgcgt gaaagattgt gggaaaaggc ggctgaaaag ggttcgtact
    13261 tgattccaca attggaaaaa ttcgcggcgc agtatccgca aatttatgag aaaataacag
    13321 gcaaaggcct tctgatcggg atgcatttcc aaagcccgga gatcggttat aaagttgctt
    13381 cggggctttt caagcgtgga gttctggtgg ctggaacgtt gacaagcgcc caaaccattc
    13441 gcattgaacc gcctctggtc gttacaatgc agcaaatcga tatgctgctg gaccgccttg
    13501 gcgatgtttt aagtgaaata agcaagacaa tttgatattc catactcatc tgaggagaag
    13561 atagatgacc agtgaatttg cagttgaatt gcgcgatgtt gtaaaacgtt ttattacccc
    13621 cgaagggaac gaaatggctg ccgtggacca tgtgaccatg cagatcaaga atggtgagtt
    13681 cttttccatg cttggctctt cagggtgcgg caagaccacg tccttgcgta tgatcgcagg
    13741 gttcgagtgg cctaccgaag gggaggtgtt cattgaagga aaaccgatgg ggcatacacc
    13801 gccttttcag cgcagagtca atacagtatt tcagagttat gctttgtttc agcatatgac
    13861 catttaccag aacgtcgctt ttggtctgga aatggaaggt gcaaaagacg aagagatcaa
    13921 aaaacgtgtc gggcgtgcct tggatatggt tcagctcact ggcatggatc gccgcaagcc
    13981 caaacaactc tctggtggtc aacagcagcg tgtggcgctt gcccgtgcct tggtgaaaac
    14041 ccccgaagtg cttttgttgg atgagcctct cggcgcgttg gatttgaaat tacgcaagga
    14101 aatgcagctt gaattaaaag cactgcaaca acagcttggc atcaccttta tctacgttac
    14161 gcacgatcag gaagaagccc tgaccatgtc ggaccgtatt gcagtgatga gcaaggggaa
    14221 agtacagcag atgggtacac cggtggaagt gtacgaacgc cctgccaaca agttcgtggc
    14281 agacttcatc ggggagtcaa acttcctcga aggcaagatc aggtccatct cacagaatga
    14341 ggcgacggtt ttcatccccg gtttgaacgc cgagttgacc ggtatgcctg tctctgaagg
    14401 ccttgtcaaa ggcgaagagg ttcatgtaag cattcgtcct gaaaaggtgc gtgtggctga
    14461 gaaaaatgca ttgaatcaga atatgttcca tgcgaaggtt acgaacacgg tttacattgg
    14521 tgcagatacc catatttacg tggaagcaca tggtgtgaag ttaaaagtgc tcgagcaaaa
    14581 ccgtatttca cgccttgacc ccaaatcgtt ttactcggtg gggcaggagg tttggttgat
    14641 gctcatgccc gaaaatacgc tgattttgaa aaaggattag cagccatgct tcagcgatta
    14701 cgtgaaagca aatctgcaca gggcacgctt cttgcattgc ccaccacggt gtggttgttt
    14761 gtgttgttga tcattccgtt gatcttgacc ctgatcgtca gttttggcag gcgcagcccc
    14821 gatggagatg tgatctacac cttctcgttg gaaaactaca tccgcctgtc aggttttagc
    14881 accgattgcc ccgatgggca ggcttcctgt tttgacccgc tgtatttggg tattttaggc
    14941 cgttcacttt cattggcgtt caacaccact ttgtttgtga tcactcttgc ctacccgttg
    15001 gcgtatttta ttgcccgtgc tcacccgaag cagcgcaata tgtacctgtt catggtgttg
    15061 attcccttgt ggaccaactt tgttatccgt gtgtatgcat ggatgatgct gttgcgcaag
    15121 gaaggcgcga tcaatgtcat cattggctgg gtgatgaatc tgcttaatat tccattcacg
    15181 ccgcttgaaa tgctttacac cccaggcgcg gtattggtgg gaatgatcta cgaattcctg
    15241 ccgttcatga tcctgccgat ttacacctcg cttgaaaaga tagacaactc tttgtacgaa
    15301 gccgccgccg atctgggcgc gaattctgtc aaaacctttt tgcgggtcac tttgccgctg
    15361 tccatgccgg gcgtggtggc agggacaatt ctcgtcttca tcccggtgat gggaactttc
    15421 atcgtatccg atattctcgg cgggcgacag gtgattctcg tcggtaactt gatccagcgt
    15481 cagttcctcg atgcacgcga cccgacattt ggttccgccg cttcgcttat tttgatggtg
    15541 cttaccctga tcgtgaccta cttctacact cgtaaattcg gatttggtga ggagattgtg
    15601 gccgcatgaa cccttttcgc cgttccccat tcgtgattgc cgcggcaacc ctggtatatg
    15661 tctttttgta cgcgccaatc ctggttttga ttttgtactc cttcaacgca tcgcggacaa
    15721 acgtcacctt tgaagggttt atcccttcct tcagtgatcg ccttgaaatg gatggcagcc
    15781 ttgtaaaagc gagtccctgc ggaccgttcc actggtattg tgatctggcc aacaatgatg
    15841 atgtggcgga agctgctggc aacaccctga ccattgcttt cacagcaacg atcgttgcga
    15901 ccatcattgg cacaatggct gccctcgccc ttcagcgcta caacttcaaa atgaagccgt
    15961 tttcgcagtt gtcgctttat atccccatcg ttatccctga gatcgtgatg ggtattggta
    16021 ttttgacctt gttcagccag ttctttggtt gggcaaatga agccttcaac ctgcagggtg
    16081 atgcgaggtt gtcccttggt attgtgacgg tgacggtgag tcacattgct ttcatggtgc
    16141 ctttcgtgac actggtggtg caggcccgtc tccagggctt tgacaaatcc tttgaagaag
    16201 ccgccatgga tcttggcgcc aacgagtgga tcaccttccg ccgtgtgact tttcccatga
    16261 tcctgccggg ggttctctcg ggcgccttgc tggccttcac cctttcgctg gatgactttg
    16321 tgatcacctt ctttaccaat ggaccaggtt ctacaaccct gccgatctat gtctatggtt
    16381 tgttgagaag gatcatcact ccgcaggtca atgcgctttc aaccgtttgg attttggtcg
    16441 tttttacggc tgtattgctg ttgcaaattt tgcaaaaccg tgaatcgaaa caacattcct
    16501 aaacttaatt gggtattccg tgcttcgaaa agcgcgtgcc aaatgacttc aatacttcta
    16561 aggaggaaga gatgagtaga aagaacctat atggattact gggggtgttg gtcatcgcca
    16621 gcatgatctt aactgcctgc ggcggttcgg ctgccccagc caacgactct ggctctggat
    16681 ctgaaggtcc aaaagtaact tcgacaggct ttgaatgccc cgagcccagc ccgcgcatgg
    16741 aagttacctc tactgagctg aatatttttg tatggaccga atactttccg caggacatgc
    16801 ttgattgttt cgaattggtc tacggcatca agctgaaccg cgatgaatat tcaagcaacg
    16861 aagaaatgta tgccaagatc tctgctggcg gctcttcgta cgaccttgtt caacctactg
    16921 actacatcat tggtctgatg atccgtcagc agcttttgca ggaacttgat cactccaaac
    16981 tgcccaacat ggccaacatg gattcgggct ggatggatca atctttcgac ccggggaaca
    17041 agtacaccct tccatacttg ggcggcacag atgcgattgt ggtgaacact gcaaccgtca
    17101 agaccgttcc cacttcatgg gcagacctct ggaatcccga atatgcgggc cgcatgatct
    17161 tcctggatga ctcccgcgct gtgatcggcc tcaccctgtt gacccttggc tacgatgtca
    17221 acacgaccga ccctgcccag cttgaagaag caaaagccaa actggccgag ctcgttccca
    17281 acatcaaagt gttcgatagc gacagcccca agaccgccct tatcgctggt gatgttgacc
    17341 tcggcatgac ctggaccggc gaagccttca ttgccaatca ggaaaacccc gatattcagt
    17401 acgtctatcc gtctgaaggc cccattcttt ggcaggataa ctgggctatt cccgtaggtg
    17461 cttctcacac cgatgccatt tatgcctggt tgaactacac catgcaggga gatgtcttct
    17521 ggctcaccat ccgcgatttc cagtatacca accccaacaa ggctgcactg gaatacgcca
    17581 aggccaacca gcctgatgtt tacaaggcct acgccgattc tccgatcacg aacccgcctg
    17641 ctgaagcggt tgcaaatggc cacggcattg aagatgttgg cgatgccacc ccgctctttg
    17701 atgatatttg ggtggaagtc aaaggcggac aatagaataa tttggcggga ttttcccgtc
    17761 gccatgcagt acaataacct caatgggtag tttgcccatt gaggttattt tttataactc
    17821 cctgttgtat aatccctttc cccgtgctct gaaaaattca aggatcaggc tgaaaacccc
    17881 acggattttg cgcatagtcg ctgaaaacga cgtcttttac gttacaaaaa ataatgtttt
    17941 tatgacaaat agttataaaa ccattgactt ttatgcaaaa aaggaataga ataaatcaat
    18001 ggaaagttta atcggtagtg gtaatccttc cagccgcctt gataacattg accaatacat
    18061 tatcgatgca atgcgaaaag atggccgcga agctttcgct caaattgccg agaaactcaa
    18121 cgtctcaccc ggcatgattc ggcaaagata taaccgcctt gtcgagttgg gttatctcaa
    18181 agtcgttgca gtgaccaatc ccctcatgat ggggatccgc accatggccc tcatcggcat
    18241 ccataccgac ggccacaaaa tgctgcaagt tgccgaggaa atttcgaaaa tcgatgaggt
    18301 tgtctacctc gtcgttgtca gcggcagata cgacattgtc tgcgaagttt tttgccgcga
    18361 tcatgaagac ctgctcaaat tcctcaccga aaagcttgcc caagtggatg ggatccgtga
    18421 aacggaatcc ttcatccatt tgaagatcat gaaagaaatt tatttctaat gggaatcccg
    18481 cctcagtacg gacctggtgc ctgctgcggc gttccaaaac tttgtctgcg ctggaaatga
    18541 gtccagaccg cagagccaac tgaaaggttc taatgaaagc cctagttcgt gtattaaaga
    18601 ctgtggggga taccttcttg aaagtgtata tgacctccct ctccatttcc attttccctg
    18661 gaaatgaatc gcctcgacaa tatcggtagt cacagtctat cccgccgtca taaaaacggc
    18721 gggattcttt ttcggtcaat ctaactcgca gatgcgggtt ataaaacaaa atggagaatc
    18781 agatctcatg aaagtattgc tagttggtgt tggtggagtg ggtgaggcaa ttgccgcaat
    18841 tgcaaagccg cgcgcctgga tggaacaaat ggtgctggct gattacaacg tcaaacgtgc
    18901 aaaggaagtt cagaagaaac tcggtgaaaa gaagcgtttt cccgtagagt tcattgatgc
    18961 cagcaagaag tccgaaattg tcaaactggc aaagaaatac aaggtcgatc tcatcatgaa
    19021 tgcggttgac ccgatcttca acgaggagat ctttgatgcc gcctattccg ccggggtgat
    19081 gtacatggat atggcaatga ccctctccaa accgcacccc aaagacccgt ttaacaagtg
    19141 cggtgtcaag ctgggtgact atcaatttgc caaagccaaa gactggaaga aaaagaaaca
    19201 gcttgcactc gttgggatcg gtgtggaacc cggcatggcc gatgtgttcg cccgatatgc
    19261 cgctgaacac cttttcgatg agatcgaaga acttggcatc cgtgacggtg caaatatcgt
    19321 catcgagggc tacgagtttg ctcccaactt ctcgatctgg acaaccatcg aagaatgttt
    19381 gaatccgccc gtgatctggg aggaaggcaa gggttggttt accactgctc ctttctctga
    19441 gcctgaagtc tttgatttcc ccgaaatcgg ccctgtggaa gtggtcaacg tggagcatga
    19501 agaagtgttg ctcatgccgc gctgggtcaa gaccaaacgt gccaccttca aatacggcct
    19561 cggcgaccag ttcatcggtg tgctcaagac cttgcatatg cttggtcttg ataacaaaga
    19621 gaagatcaac gtgaagggtg tacaggttgc gccgcgtgat gtagtggcgg cctgcctgcc
    19681 tgaccccgct catcttggcg acaagatgtc gggtaaaacc tgcgctggga cgtgggtcaa
    19741 aggcaccaag gatggcaaac cccgtcaggt ctatctctac caggtggctg ataacaaagc
    19801 ctgtatgaag gcttggggtt gtcaggctgt tgtggcacag accgccttca gcgcagtcat
    19861 tgccatggat ttgttggagc acggcatttg ggatggggtt ggtgttcttg gccccgaagc
    19921 cttcccgcct gatccattca tggaaaaaat ggccgattac ggcttcccgt acggcatcaa
    19981 ggaaatggcc gcataaagta aacaaagcgg acaatcgtga gagcgattgt ccgctaattt
    20041 ttttttcagt gcaataaaaa atttcggagg gcaaaatgaa aaacaattct ggggagggaa
    20101 atgtgatcag cgctgagacg aagaaactca agcgcgaact gacgctgttg ccgcttttcg
    20161 ggttgatcta tttcacagtg tgcggtgggg catttggagc agaacccatg gtcggttggt
    20221 cgggaccggg gttggcgctc ctgctgttca ttttgacccc gcttttcttc agcatcccca
    20281 atatgttgat ggtacgcgag atgcaatcca tgatgcctgt ggagggaggc tattaccact
    20341 ggctgaaggt tgcctttggt ccttttgtcg gctatatggg tgggtggatg aattgggtgg
    20401 tttcatgggt ggatgtatcc atttatcccg tactggcggc gtactatctt ggttatttca
    20461 tccctgcttt gcgtgagggt gcggtaatcg gcggtgtttt tgtggatagc acctggcttt
    20521 cgtttggggt ggctgttgtg ttgatctggg cggtctctgc tttgaatatt cgcggtgcgc
    20581 gtttctcggg tctgctgacc aactggcttg gggtggtgat gttgatcccg ttgattgccc
    20641 tgactgtgat cgggttcatg gcgtggctga aaagcggtga gaccattcac cttccctttt
    20701 tgcctggcgg tgcagaggtg aatttcagca ataccatcgg cgcgttgagt acgggcattt
    20761 ttgtagccat gtggaatttc atggggtggg aattgcctgc agccgctggc gatgagatcg
    20821 tcaatccgaa gaagacctat ccgctggcga tggttttggt gttggtggct gccattgcta
    20881 catattccct gcctactttg gcgggtttgt atggcggtgc tggtgaagat ggccgctatc
    20941 aaatgtgggg tatcgaggag tatgagagcg gcgagggtat tggtccagta ttggatgagt
    21001 acggtgtccc tgctgagcag gcccaatcct ggggcgtgga tacagccagc tcggtcggat
    21061 gggaatttcc cgatatcgcc catgtcgtgg cggataaatt gacgggtcaa accaacggga
    21121 cgcttgctct ggttatgggt gggttggtca ccttttcggc agtgctgagc atgatcggtt
    21181 tgttcattgg caatgggctg ggtggaaccc gcgttccgtt tgcgatggca gcggatggga
    21241 tgatgccgaa gttcatggtc aaggtgcatc cgagatatgg cacaccctgg gtggcgattt
    21301 tgttttgcgg gttgatctat tcgatctttt cactttcagc gtttgcggct ttggttgtga
    21361 tcgatgtgtt cctcaatatg ctggttttga tggccgaatt tttcgccatg tggaagctgc
    21421 gtttcaccat gccgcatttg gaacgaaagg aagtaccagg cgggtatttt ggcctgaccc
    21481 tggtgacaat tgctccggcg atgatcattg gacttgccat ttacagccag tatgtggaag
    21541 agggaatggc gtccatttca tgggcgttgg gagcaatggc agttggcgcg attttgtact
    21601 tcccaattcg aaaatggatc aagccgggcg ttccagatgt gaatccgttt gtggcgggcg
    21661 aggaagattg aagcaggtaa gacgtgctca aacgcaggga aatgcccctc gaaaccgaat
    21721 caacttccag ttaagcgtga ttcggcgcgg gtcgtgtaag aaaaaactta tttgtgttga
    21781 atctctgtcg ctttcggaaa tttcccgttg tgcgaacctt tgagcgcttg ttataatcca
    21841 acaaacaaaa tgatcggcgc attgaacatg cgctttgatg acgttctaca cggaacaccc
    21901 atattcatac ggaggagctc atgcccaacg gttataacgg aaagattttg cacgttgatc
    21961 ttacgaaagg ttcgctttcg gtggaagagc cgaacgaggc tttctatcgc aaataccttg
    22021 gcggcagcgc gatgggaatg cactacattc tgcgcgatat gcccaaaggt gccgacccgc
    22081 ttggccccga taatgtgttg accctgttca ccggggttgc cacgggtgcg gctatttctg
    22141 gtcagagccg tttgaatgca aatgccaagt cgccgatcag cggcggtatt ggcgattcgc
    22201 aaagcggtgg tttctttcct gctgaattga aatttgctgg ttttgatggg atcgtggtca
    22261 agggaaaatc ttccaaacct gtttaccttg ccattattga tggaaactat gaactgcgtg
    22321 atgcggcgca tttgatgggt aaactgacgg gagaagtgga tgacatcatc cacaaggaag
    22381 tggacccgaa ggctgaaatt ttgcagcatg gcattggtgc tgaaaacggt gttttgttct
    22441 cctcgctgtg ttcaatgtcc aatcgacaca atgggcgcac ggggatgggg cttgtgatgg
    22501 cttcaaagaa tttgaaagcc gtggtcgtgc gtggcacgaa gaaggttcag cttgccaacc
    22561 agaaagcatt gaccgagttg aatcgcatcg gccccaaggc cattcctgaa aatggagata
    22621 tggacggcct tgcgaaattc ggtacggcgg ttgtggtgtt gttcaacaat accatcggca
    22681 cattgcccac ccgtaactac aacgaaggtc aatttgaagg ttgtgagccg atcagcggcg
    22741 aaaagatggc tgagaccgtt ctgaaggaac gtgatacctg ctatgcttgt gtggtcaaat
    22801 gcaagcgcgt ggtggagatc aaggacggcg cgtacaaagt ggatccgcga tatggcggcc
    22861 ccgaatacga aacccttggc acttttggtt cctactgtgg tattgatgat ctcgcggcgg
    22921 tttcgcttgc gaatcagatc tgtaacgaat acgccgtgga ctccattgcg gctggcgcaa
    22981 ccatcgcttt tgccatggaa tgttacgaga agggtgttat caccaaagaa caaactgctg
    23041 gcctcgacct caagtttggc aatgccgatg caatgatcaa ggctttgtac atgctggtca
    23101 aaggcgaagg tgagtttggc aagacccttg gcatgggttc tgaacgtgcg gcaaagaaat
    23161 ggggcaatgg cgcggaagat aacctgatca cggtcaaagg cgcggaagct cctgcgcaca
    23221 tgccgcaggc caagcgttcg cttgcgctca tttacgcggt caatcctttc ggcgcggatc
    23281 accaatccag cgagcacgat ccctactacg aagaaggtgt tggcgatttc aacctcgacc
    23341 gcctcaagca gatcggtctc ggttcgcccc aacccgcata cagcctcacc gaggaaaagg
    23401 tccgctttgc gtatgagacg gaggttttct actccatgct cgattccgct gaactgtgtc
    23461 agtttgtgta cggccccacc tggaccctct atgacggcaa ggatactgcc aacatgatcg
    23521 gctcagtcac tggttgggat atcaccgttg aagaactgat ggaagttggc cgccgccgtt
    23581 tggatctttt ccgcgtgttt aacgcgcgcg aaggtctcac ccgcaatgcg gataaactgc
    23641 cgaagaaatt cttcaagcag ttgcagggga ctggccccac ggctggcttc gctctgactc
    23701 acgaagaagt tgattctgcc atcgacactt attacaagct cgcaggctgg accaacgacg
    23761 gcgttcccac ccgtgagacc ttgaagaagc atgatgttga gtgggctgcg gaatacctgc
    23821 ccgcatagaa agcgaatagt agtaactggc aatttgtaat tagagattgg agatgggatg
    23881 atgagtctca tctccaattt tcggtaattg caaaattttg ctttagaagg aacgtccatg
    23941 aaccaactct atcaagataa tttctctcat atgacccccg cctggagccg tattttcagt
    24001 tttgtatcag aacgtgctga gggcgcctat atttatacag atgacggcaa aaagctgctc
    24061 gatttcacca gcggcattgg tgtcacgaac acaggccact gccatccgaa ggtcgtggaa
    24121 gccatccgtg agcaggctgg gttgtttttg catgcacagg ccaatattgt tgttcataaa
    24181 cccatgctgc agttgattga cgaactgcgc aaaattgtcc cgccttcgct cgatagtttc
    24241 ttttttgcca attcaggtgc agaggcattg gagaacgcgg tcaagatcgc gcgtgtggca
    24301 acgggccgac ccaacgtcat tgttttcaat ggctctttcc atgggcgcac acatgccacc
    24361 atggctttga ccacatccaa gaccatctac cgcacaggct ttgctcccct gccttcgggt
    24421 gtatatgtca ccccatttcc ctatgctttc gccttgaaaa tgacggagga acaggcttct
    24481 caatatgcgt tggatcgcct ggagtatttg ctggcttccc aaacagcgcc aaaagagacg
    24541 gccgccattc tcatcgaatc tgttttggga gaaggtggtt atgttgtccc gccgacatcc
    24601 tttatgaagg ggctgcgcga gatctgtgac aagcatggaa ttatgctgat cttcgatgag
    24661 gtgcagtctg gttttgggcg cacaggcaag tggtttgcgc tggaacattt tgatgtaacc
    24721 cccgatatca tcaccgccgc caagggaatt gcctctggaa tgccgctttc gggtgttttc
    24781 agccgcaccg acattatgaa gaaagttgaa acaggatcca tcggcggcac gtacggcgga
    24841 aatgcggtcg cttgtgcggc tggcgttgcc accatccgtg ccatgcggga agaaaaaatg
    24901 ctcgagaacg cctccgaacg cggaattcag ttaatgacag gcctgcgaaa gttgcaggaa
    24961 gagtatccgc aaattggcga tgtgcgcggc aaggggctca tgatcggtac ggaattcatt
    25021 gttgacggta cgcaggccaa ggcgaaaccg ctcgtcaaag agatcattca caaggctgaa
    25081 gaaaaaggga tgctcctgct ctcctgtgga acctacgaca atacccttcg ttggattccg
    25141 cctttgaatg tctccagcca gcagatcaat gatggcttga agatcttcgg cgaggccttg
    25201 aaggaatcgg tgtaaaaaaa aggagatggg ccgtacattc ccatctcctt cttctggtga
    25261 cttggttcta aaagttagac gaacccgatc actttctttg atttgtcttt gaagtcgcgc
    25321 ttgtcgaatt cgaagatatc tgccagggtt aggcttcctt ttacgtcatc atgtccaagc
    25381 tttttggcga gggcggctgt tttctcggca gacagggctc taaagttgta ttttgcgacc
    25441 attcggcctt ttcgcagcag agccttgtcg atgcgttcaa tatccgtgtt gaaggtgcag
    25501 atgatcttga tgttgaggaa atcgctgaac aaaccatcgg tcaactgaag aatcgtcgag
    25561 acgaccgaat cctctgatgc gttatcgcgc gatacgacca ccttttctgc gtcttcaatg
    25621 atcagggatg cgttcttgtt ctggagcagg aaggaaacaa aagacggttt cagcaggtct
    25681 cggacgaaat cgttttgaac gaaaatgaaa ttcttgtcat ggaaccttga aatcaaatgt
    25741 ttgatgtagg ttgtctttcc agtgccaggt tcaccatgca gcagaatgat gcccgattcc
    25801 ttctttgcca taaagtctga gatcgttgcg tcgatctcca gaaaatcatc gttgtaatac
    25861 tctgcaaggt tgatggactc gaagtcgttg gtgttcacat cttcagtgcc aaacatgccc
    25921 tcttgctgca tcaacacctt gaattttggc tgcttggcag ggctgaagcg ttcccgcagg
    25981 ttttggttgg tttcaacgac ccacttctcg cagtccgggc ggttcacatc gtaataaaaa
    26041 tccacggtga tgaacccttc acgggcaatg agatggacca gcatttggcc gctgtcgctt
    26101 ttgaaaagat actcgtatgg ataatcgtca atgggacgcg cgttcttgaa atcttctgta
    26161 gcccagtttt tgaagatcgt aatgaacgag tatctttcaa ggtccaggca gtcaatgata
    26221 tcgtccacaa gcgctctggt cctgtcagta aagacggaga agcgcaggcc ggatgacagg
    26281 ctgccaaaac agaccgtgta gtaggctggc accctgaaac gtttgtcttg attgaaagta
    26341 tcgagcaagg ttcctgtagc agtgttttcc atgttttcct cggtagaagg tgcgggtttt
    26401 tgcttaatac ccgcccatac tgaaatataa agttgattcg atatattggc aatcctaaaa
    26461 attcatttgt aatggacaca aaatgacaaa atcacttgat cgctccaaac tcgaaacact
    26521 tctcaagcag gaagaggaac tatttcacaa gaatcacccg agatcctacg aaatgtacca
    26581 gcgtgcgcgc aagtcgctgc atggtggcgt gccgatgttg tggatgatcc gttgggcggg
    26641 ttcctttcct gtctttgtca gggaagccaa aggcgcccac tttaccgatg tggatggcaa
    26701 cgactacatt gacttttgcc ttggcgatac aggtgccatg acaggtcatg cgcccgaagc
    26761 cacggtcaaa gccatcactg aacagatcca aaaagggatt accctcatgc ttccatatga
    26821 agatgtgatc tgggtgggtg aggaattaca acgccgcttc aaactgccct attggcaatt
    26881 cgccctcacc gccaccgatg cgaaccgatt cgcgcttcgc atggctcgtc ttatcactgg
    26941 ccgatccaag gtgttggttt tcaactattg ctatcatggc tccgtggacg aaacctttat
    27001 cacgctggat gaggaaggta cgcccatctc acgtcccaac aacatggggc cgcaaattga
    27061 tccgcgcgag accagcaagg ttattgagtt caacgatatc gccgctttgg aaaccgccct
    27121 ctccgcccgc gatgtggctg ttgttctggc agaacctgcc atgaccaaca ttggcatcat
    27181 tcaccccgac cctggttatc acaaagccct gcgtgaattg acgcgcaaat acggcaccta
    27241 tctcatcatt gacgagacgc acaccatctg tgctggcccg ggaggataca ccgcttcgca
    27301 ctccctcgag cctgatttct taacccttgg aaagcctctt gctggaggtg tccctgccgc
    27361 gatctatggc ttcaccgaag aagtctccaa ggcgtttgcc gccaagctcg ccatggacga
    27421 tgccgatgtg ggcggaattg gtggcacact ctctggtaac gccttgtcca ttgcggcgat
    27481 gaaagccact ttacagcacg tgttgaccga tgagttttac gcaaaagcaa ccgccttgca
    27541 ggagcaattt accgcagggg tgcagtccgt gatcaaggaa ttcgagttgc cgtggatcgt
    27601 gaagcagttg ggcaaccgtt ctgaatactg gttccgcccc aacccgccga aaaatggtgg
    27661 agaagcccat gctgccgttg atcctgaact cgaccgttac atgcatctct atgccttgaa
    27721 ccgcggaatt ctcatgaccc ccttccacaa catggctttg atttctccag aaaccaccca
    27781 ggccgatgtg gatcatcaca ccagggtgtt tcgagaagca gtggcctcgt tatttgctta
    27841 accctacctc gttactggta taacgggttt tgcaaacttt ttgaaaataa atgcgaatat
    27901 aatcctgctc acaggagtca aaatggctga atataaatct caaatttggc gggtgaacac
    27961 ccgaacccag gaactcaaac gtgaaccgat cccagaaaca tggcagcgtt tgggcgggcg
    28021 tggtcttatc gcgcgcatcc ttgtcgatga ggtggatgca aaatgtgatc cgcttggtgc
    28081 tgacaataaa ctgatcttca cgccaggttt attggtgggg cacatgcttt cttccacaga
    28141 ccgtatttcg gtgggcggaa aatctccgtt gacgggcggt atcaaggaag ccaatgcagg
    28201 tgggcgcaca ggctatcaca tggccttcat gggcatccat gcgctcatta ttgaagatat
    28261 gccaaaggaa gacgggtatt gggtattgca cctctcgctg aatggcgcaa aatgggaacg
    28321 cgcggatgac ctggctgggc tgggcgttta cgcaaccgca ccgaagcttt tggaaaaata
    28381 tggcgacaaa gtggccattt ccatgatcgg gcctggtggt gaaatgcgta tgaagtcggc
    28441 agggatccaa aacctggaca aggacaaaat cccttcgcgt atcgcggctc gtggcggcct
    28501 tggcgcagtg atgggttcaa agggattgaa ggcgatcgtt ttcgatcatg caggcggtca
    28561 gaaacctcca attgcagatg cagaaacatt caaggtggcg caaaaggatt acacccagtc
    28621 tgtgatgaat cacccgcaat ccattacgta tcgtgattat ggcacccagg ccatgccgca
    28681 gatgactcag cgttttgcag cgttgcccac ccataatttc tcacgcggca ctttctacga
    28741 tgtggaaaaa ataagcggcg agaccatgcg tgaattcatt ttgaatcgcg gcaagccatc
    28801 tgacccgtct catgcttgta tggcaggttg taccattaaa tgctcaaacg ttttcggcgg
    28861 agaggatggc aagatcatcg tttcaccgtt ggagtatgag acggttggtt tgatgggcac
    28921 caacctggat attgattcgc tcgactcgat cgggcgcatg aactggcagg tcaatgatct
    28981 tggtctggat accattgagg ttggcggggc tttgggcgtt gccgctgaag caggcctgat
    29041 gcgttggggc agcgaagagg atggcctgaa gttgatcgat gagatccgtc agggcacgga
    29101 acttggacgg gtccttggcg atggcgccgt ggcggttggc aaaaaatatg gtgtggagcg
    29161 tgtccccgct gtgaagggcc aagccatgtc ggcgtatgag ccgcgttcca tcaaggggac
    29221 tggtgttacg tacgcaacca ctcctcaagg tgctgaccat acctgcggcc tcaccatccg
    29281 tgcgcaggtc aatcacctgg acccgaccca gcaaaaagat gcttcgctga atgcccaatt
    29341 gaatatggca gggtacgaca ccatcggtgc gtgtattttc gcgggctttg gttatgccgc
    29401 cacgcccgat ggagtggtaa aacgactgct caaatcacgt tatggctggg aagatgtgcc
    29461 cgacaatatt ttgcaggcct tgggcaggga aaccatcaag ttggagcgtg agttcaacaa
    29521 gcgggctggt tttacgaaag aagatgatcg cctgcccaag tggatgacag aagaagccat
    29581 ccctgaaaac ggatcggtct ttgatgtgag cgaagatgtg cttgatcaca ttttcgatgg
    29641 cattgagtaa aagttgttta caggtcacgc ttaaggcgtg acctgctttt taagccgata
    29701 ttgcatcaag aaccagccga cgacaccgaa caccaacgaa acaagaatcc cttcgttggt
    29761 gggggtgaag acgggagcca atgcaccttt gacctcgata aatccgttga taagcagggg
    29821 catgctcaag gcgttgatta tgttatgcag gatgaacaca ggccaaaccg atttgctcaa
    29881 caacctgatt tcaccgaaga gaatcgctgt ggggaacatg acaagatagc ctgtgagcag
    29941 aaaaacagga atgctggttg taatggcgct gttgagggtg gcacggtcaa ggtaataata
    30001 gtaataaggc agatgccagg tcatccagag caggctgacg atgaaatgat tcagcatggg
    30061 gtggagtttt gcggcttcga agcggggagt gagataacca cgccaggcaa attcttcgaa
    30121 aaagtttttc atcagcgaac ccacaaatat cattcccgct gcagaaagat aggcgttgaa
    30181 gccctgggcg gcaaaccctt cggcgctgac gatgccaaat agggcaccca gaccaaaggt
    30241 caggagtgaa gcgagcgggt agacgaggat accgagaagg taccacttcc agcctgagat
    30301 gagatttagc ccaaagccag aatccttcca gccatcgcct ccgaaagcgc gcagtaaaaa
    30361 gcctgaaaag gctggtgtgg taagccaaac cagtacgccg aggctttgca tgggcggttg
    30421 ggtgttgcct gtgacgttgt tgagccagat gcccagccag ccgccgccga ttgtaaagag
    30481 tgaaacgatg ataatgttgc gaatggtctt gttcatttat tttcccatgt tgatcgtgta
    30541 atttttattt tcaatgcgtg caatggctga gcgcagccat gccgcgatgg attgcgtgtt
    30601 gatgtatcct gcttcgaggg taagcatgct caggaaaacg gtccgtgggt cgctgacctt
    30661 tgaaggtgtg ttctgggtca tttgaaaaac cgcttcgtac agagccagtt gctcttcgat
    30721 ttttttgagt ttgtcttgaa agactttgag caattctgtg tcgttgagtt gaccgccaaa
    30781 atagacttga atgaggaaag gttcacggtc atcgcgttcg ggcagggggg cggcaagcca
    30841 ttgatgaagt tcttcgcgcc ctgtggcggt gatgtgataa accttcatat ccagccgttc
    30901 ttcgcgggcg atgacctctt tttctacaaa tccattttct tccaattcgg ccagagtgcg
    30961 gtagatttgg ctttggttgg ctggccagaa atgctgtaca gagttgtcga aggctttttt
    31021 caggtcatag cctgtgaacg gggcaaaaga taaaaaaccg agaatggcat gctttagaga
    31081 catttttgtt ccttgcgtgt agttttatat tacatatact ataatactag tattatagta
    31141 tatgtaatat atttgtcaat aggaaaaaaa taccctgaaa aatcagggta ttttatgaag
    31201 atgatttgtc tgtcagtgtg aagtaaaagg tcgttcctga gccgtagccg tacgagtcga
    31261 tccaaatgcg cccgccatgt acttcaacga tccgtttcac cagcgccagc ccgatgcctg
    31321 ttccactgct ggtggggtcg agcttgtcga acagcccaaa cacacgttca tggaaatcct
    31381 ggcgaatacc ggggccattg tcttttacga agaagatatt ttcgccatcc cgtttttcga
    31441 caccgatctc gatgatgggt ttcggctggt cgctcatgaa tttacaggaa ttctctacaa
    31501 gattttgaat gacttcgatg agacgttcct tgtcgccgtt gatctgaggc aggtcgcttc
    31561 cgatcttgac ctgaatctgc ttctctttca attggctttc cacgcgcttc aagccttctt
    31621 ccacgacttc tgaaaatgag atgttgcgcg gttcgttgat gatgtgccca acccgcgaaa
    31681 gttcgagcag gtcattgagc aggttgtgca ttttttcagc ggcctcggaa atgcggccaa
    31741 tatcctgttg cagcctgtca aaattcccgt tgcgggcatc tttttcaaga tagcccagat
    31801 aaccacggat ggtgatcaac ggagatttga ggtcatgtga agctgtgtaa gtgaaacgtt
    31861 ccaactctgc gttcttttgc tccaattccg tcaccagctt ttcctgaatg gtcaattccc
    31921 gtttgagcga ttcttgcagg cgggtatttt cgattgcaac ggcaaggttg gcggcgatgg
    31981 tggaaagcag acgcaggtcg ttttcggaga aggcattttc tttttccata tcgtggacgg
    32041 aaatggcgcc aatgcctttt tcgccgatca tcagcggaac ggtcagggaa gaacgggcgg
    32101 ggctgttatc gtagtaaatg acgccatact ccgccgagac ttgtgcccag tttgtattga
    32161 tcaagaatgc ctttttcgtc cgcaggacac tggaggtcat ccctttgccg aaagtcagag
    32221 agggagcctg aatttgctgg cctttctcga cataatatgg gaaatggatc aggcttgtag
    32281 tggcattcaa cagggaaata aagaggttat ccacattgaa tgtgaccttg atctggtcgc
    32341 ctgtttgttg gatgagttct ttcaggtcgg tttgggcaat gagagtctca ctcacaccgc
    32401 ggacggcatt caattcggcc agccgttttt gcaattgatc ttcggccatt ttgcgggcgg
    32461 tgatatccac cagaaatccc tgcaggacat tattttccat ggagcggatg ctttggtctt
    32521 gcacccagac ataattgccg ctgcgggttt gcaggcggta ttcggtgcgg ggcatttgtc
    32581 cgctgttgag cgaggagagc tccgcttcca cggtccgttc acggtcatcg ggatgaatga
    32641 ggcgatacca aaggtctttg ttgagggtcc attcttcggg agtgaaacct gtcaacgcct
    32701 cgatctgcgg gctgacataa tgccaaatgc ccgccgcccc aggctcggaa atgtaaacca
    32761 ccgctgggat ttgctcaacc aggttgcggt agcgcgtctc ggcatcacgg agctgctttt
    32821 caatggtctc gcgttcgcgt atttccaact cggcggtttt gatattttca ttcaggcggg
    32881 cgatgacaag ccactgaaag acggtcacca gcaccacaca caggcttgtg gagaagtaat
    32941 cggcaaaggt gttgaaatga ctgaatttgt ttgtgatgta tccctgtgtt tcgaggaagc
    33001 cgaacaaaat cacttcggca aggataaggg cccccacaat aatggtccct ctgcggcctg
    33061 ccagcagccc ggctgcgatc aaaacaacca ccaaacccaa aatggcagga tctcggatgc
    33121 cattattctt ataaaccagg ctcgaaatga tggcgagtgt ggtgaatagc gaaacccacc
    33181 gagcggcatt taagtatccg aaatacccca gaaccagagc gatgaacagc aagaggctga
    33241 gcgggatcat ctcagcacgg ttgatgttaa tggcagccat gagcgccgca ccgaccacac
    33301 cagtgatgag ggtggccagc acgagctcgc gcgcattgaa cgatcgcgtc tttattttga
    33361 gtatttcgga gatgtattta ttcatggagc cagatatggt aattctactt taaaagaaat
    33421 aacccgtaag gtctgagcct tgcgggtcgc agggggtgat gcacttgagc caccagtggg
    33481 atacgaaccc acgacctaac gattacgaat cgtttgctct accagctgag ctatggtggc
    33541 ttattttcaa gcggcgggga ttataaagga ttaggcttaa tctcgcaagt tttcgaggtt
    33601 tcgcgtcgtc tgtaccgtgg agaattgaga aaatatgctt cgtattgcca tgttatcgta
    33661 tcacacctgt cctttggcca cactcggcgg caaggataca ggtggaatga atgtatatgt
    33721 ccgtgatctg acctgtgaat tggggcggat ggggatccat gtggatgtgt tcacccgctc
    33781 tcaggacgag catgtgccgc atgttgtgca tgaattggga tttggcaatc gtgtggtgca
    33841 tatccctgcg gggcctgaag agccgaaaag caagagcgat attgcaaaat tcatccctga
    33901 gtttgttgag ggcatcaaac aatttgccgc tgagaagaat attcactacg atgtcattca
    33961 cagccattat tggatgtctg ggttggcggc ggaggccttg agcgatgcct ggggcggtac
    34021 gcccattgtc cacatgtttc atacgttggg ggagatgaag aatcgggtgg cgcgttcgga
    34081 ggatgaacgt gcaggggaag accgactcaa aggcgagagg caggttctcg gtcgggcgga
    34141 tcggatcgtt gtcgccactt tggctgagtt gacccagttg agattcctgt accgtgcgaa
    34201 tgcccgcaaa ctggttgtca ttccgccagg ggtggatacg agtcattttt atcccattcc
    34261 tgccgatgag gcgaagcagt acattggcct gacacccgat agccgcatgg tgctttttgt
    34321 ggggcgtatt gagccgttga aaggtgtgga cacgttgatt caggcgatgt cttgtttggg
    34381 ggtgaatgaa attcataaac ctgtgcatct ggcgattatt ggcggtgaac ctgatgtgat
    34441 ccctgaaaaa atgtcggctg aaatggcgcg cctgcaaaaa ttgtgtgacg acctgtgtat
    34501 gggcggcatg gtggtttttc ttggcaaacg cgggcaggat acgctgccat actattactc
    34561 ggcggcggag gtgctggtga tgccttcgtt atacgagtct ttcggaatgg tggcattgga
    34621 ggccatggcc tgcggtacgc ccgtcatcgc ttctgaagtg ggcgggctcg gttatcttgt
    34681 gcaggatggg gagacaggat acaccattcc cgatagcgac cctgaggcat tgtgtaataa
    34741 attatctgga ttattaggtg acgcgcatct gcgcgaaacg atgggcctgc gtgcggcgga
    34801 atatgcattg gactacgcct ggtcaaatat tgccgcccag atcgtggatg tgtacaaagg
    34861 gctggtggag aagcggagtg ttggtgtttt gctcactggc tctgatgccg tacgttctta
    34921 agaactttta ggccagtttt cgtgccgtcg catggaacaa acgccgtatt cattgcgtct
    34981 gctttccatg cgacctcttt atatattttt gctttcaagt gtatttgtct ttgtggcagg
    35041 ttgttcagcg cctcagacta ccgtaaacaa cgtccccgtt tcagaaccaa caagcaatga
    35101 tttgccgcct tcttctgttg ccttcgttga tgcaaggttt gcggctgaag ccacagcgat
    35161 acagcttctg gagacattgg atggtgcgaa ctggctcgaa ggcccccgtg tcttgtatgt
    35221 tgaagagatg agttatgaac aggccaacca aaagattggc ttgggggagg gcgattttgt
    35281 tacctggcct cagggaacaa gggtgtggtt tgtcattgcc agtggaagat ggcaggtgac
    35341 acaatttggt ccgaatcctg aaatttatga agggtgtctg ttcatggtgt attcagtcag
    35401 tggggctaat attattgcta tgggtgattc ggtttgtcca accaattaat tcttatttcc
    35461 agcgcggtca tggtgttcca tacacatggt cgcgctggaa agtctcaatt tcagaacggg
    35521 taaatatata tccagcaggc ttggcttcaa ggctgttgga tatgcccatg ttgccgatcg
    35581 tgtctccgtt gagtgtgatg gtggctgtct gtacaaaggg gtttgcaaat accgtcatga
    35641 gaatttgcat acgtgcggcg atcaaggtga catcgccaac accaaaatac tcgccctgta
    35701 aaatcacgtt tgtatggccg tcgttgaaag tgacatccac tattgccacg ttggtgctcg
    35761 tccagccatt tctttcatcg cgcagaacat tttcaagcga tgctctgatg tttgtggccg
    35821 tgtcaggtcc gtatgttctg tcagagggtg ttggcgcaag gatgtaggat atgactacta
    35881 tgcttccatc gggaggtgga ttttcagcag gtgttatgaa gtagtagtga acaatccgct
    35941 ctgatattgg tatggatggg tctgtaaata gcaatggcgt ggggctggca ggtaaaacga
    36001 ccggtgcagc gggtgtagct gtgggaagtg ggtagggggc tggggtcgca ttgccgttat
    36061 ttgaaagggt ttgtatcaat ggtggcggac aatccgctac tggcgttggg gtggagacaa
    36121 aggtggatag ac
//
LOCUS       DAOQLC010000029        35946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_352_length_35946_cov_25.277173, whole genome shotgun sequence.
ACCESSION   DAOQLC010000029 DAOQLC010000000
VERSION     DAOQLC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 35946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35946
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_352_length_35946_cov_25.277173"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PL000_07085"
     CDS             <1..495
                     /locus_tag="PL000_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88697.1"
                     /translation="QNQWFERRASSMFAQIGQSKEANLTAQEMLEGEMLLDEAYLNAY
                     DKIADEDVKKYGYVKGDVEKRARDSIKDDETKIFDRSVYRMRQLEEKEMGDRKLQANV
                     GKGVESGTPLTLEMARNYIKKYGSTEKALEIAKKNGYTVPTKSELEFYQMSPQQVRAY
                     YGSR"
     gene            482..1846
                     /locus_tag="PL000_07090"
     CDS             482..1846
                     /locus_tag="PL000_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88698.1"
                     /translation="MAAAKSQKEFEEQLAFMDLAGEDESNESGDVSSRNSSEVAQPSL
                     LNDIKNYGKNAAKALAIGTHRLGRALGPTFEGDLSTKGEKGVERDFRSYLDEVFPSEP
                     GGAVTETTERFLQNAPTYLASPGASVGGTLARTAAGASAAQGVKELGGGETAQEFADL
                     LFYLTPDIGKKIIGSKKSQEWIRWARGKGLSDKEIAPLIRDGKLGNLFSHFSKKGQRS
                     RNALQNTKGAISDLYSSMRSSPEGTKVLSKPDELFVRLKEDISHMPSHVRKAINDDVN
                     KLIAKPSPTNADLLEFWQDVNAAVATDPENLRRLVNVKEPIYRHLLENSPQLAKDFEM
                     TNALYGKYSNAASKLKSGIKDQLIDAGEHGAFLYGLTTGNLPLLISVVGEGAARRLAT
                     EMLINPRFQQLIPKTVSALNQNKVSVLKHLVNLIQDEIIEEAPEAAKELASIDWDQWG
                     AGAS"
     gene            2057..3145
                     /locus_tag="PL000_07095"
     CDS             2057..3145
                     /locus_tag="PL000_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88699.1"
                     /translation="MATPSTLHAMGLEKPLIPVFPFPLYMDRAPTSSDKNFNIGQIWF
                     YKIDPDTCELYAFAGLDSTQSAIWLTGSVGPAVLSSLEAEAGDTSVSGSSGTILLAGT
                     ANQIVTTGDNTAHSITFSLDPDVVITTSLDVPLIVPTGGDLSVQLGDNIGANYLAVEN
                     ALGSRKIILDSAGVITAPQMQTTTDLTLKLSDGAGASSLYVVDDTDVQKFKVISDGQV
                     EVSNRIVAASGNLEVAGADVITTLGDSGGVNKLSIQTVASVEVASIGSDGRITGSLYS
                     FTSTYGMSGQVTLVAGTATVVNANIATTDVVILERTDINGSTALGVPVVVKNAGVNFV
                     ITAVQAGTPAATETNDVSIFNYVIMRRA"
     gene            3483..4040
                     /locus_tag="PL000_07100"
     CDS             3483..4040
                     /locus_tag="PL000_07100"
                     /inference="COORDINATES: protein motif:HMM:NF027516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-activating E1 FCCH domain-containing
                     protein"
                     /protein_id="HNC88700.1"
                     /translation="MSSSTAPWDNRPFKFGSRDRREFIVSDTEIALRAINDTNGNPIF
                     LAWAKAGSAESSAIWQLRKIQYDSNQGVTSVTWAENPDGIPTTNYEFVYASTSDLTVT
                     GVTNANPAVVTVSSIGTLQNGDLVIFQGISGMTELNFDGSNIYTVANIAGSTFELSGI
                     DSSAYGVYVSGGTANFSEVVNYTYG"
     gene            4043..5452
                     /locus_tag="PL000_07105"
     CDS             4043..5452
                     /locus_tag="PL000_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88701.1"
                     /translation="MAYKLNPFTGNLDRVDAGGSSSVTITFDADSGSATTLSNTVNLL
                     SDVTASSGTPFESTASSHTLTYTLQRSASTSTSDATLVGLAAFDSAAFDVDANGFVQL
                     NGGGIATTQYAPNSGTNPVVPTAAGLLNIVGTGSTTVVGSLNTVTIELTGLTDHNVLV
                     GAGTSTVTNVVPSATSGVPLISNGASADPSFGTAVVAGGGTGSTSFTAYAPVCGGTTS
                     TNPLQSASTGLSTSGFVLTSNGASALPSFQSVSASGAITTITGNTGGAESPSSGNFNI
                     VGTGSITVAGSANTETVQLTGLTNHSLLVGAGTATITNLGAATNGQLPIGSTGADPVL
                     ATITASTGVSITNGAGSITIAATGAGMATVEVTGTSQSMAVDTFYIANNAGLVTLTLP
                     TTSALGSVIEIAGKGAGGWKIAQNSGQQIYLGSTATTSGIGGSLASSNFADTIKIRCI
                     TANTLWTVVGAPVGNITYV"
     gene            5462..6196
                     /locus_tag="PL000_07110"
     CDS             5462..6196
                     /locus_tag="PL000_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88702.1"
                     /translation="MVTTNGINNYTPENFQVGTLSVNTNAQTGILLSKITVKGAGGSS
                     DCYISVDANSSTAHTGYGFVNSGDSTYWSWYRENTSNVMKLDYQAVNKWQVDSSGNVT
                     VANGLIIGSGSTLSSYVTGSWTPSISFGTGTTGITYSTQAGTYTRIGDFVTAYFYIVL
                     THKGSDTGNVRVNGLPYTVSSGNNAGAVATSNITYTGTYIICQGIASQTYGNLQYCTT
                     AAAGALLTNSNFANNTKIQGSFIYKV"
     gene            6419..6580
                     /locus_tag="PL000_07115"
     CDS             6419..6580
                     /locus_tag="PL000_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88703.1"
                     /translation="MDLKELQKDLHEHLDVFKKSYEDMVEVLKEKICNIICHDLPRDD
                     EPKDPPAAP"
     gene            6795..7022
                     /locus_tag="PL000_07120"
     CDS             6795..7022
                     /locus_tag="PL000_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88704.1"
                     /translation="MLEDTEDLLYEALEIRNEMAKFLEDLGADAVGALLGSNFLCMDI
                     LIQYGVPLGHFLDAQPDFYRMCLRAKRLNEL"
     gene            complement(7061..7237)
                     /locus_tag="PL000_07125"
     CDS             complement(7061..7237)
                     /locus_tag="PL000_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88705.1"
                     /translation="MKNVIKFFGGLMACGIVCAAPLSAAVNHDPVGEYSVVNTDGTVT
                     FPSGSGSDVYSVNR"
     gene            complement(7286..7972)
                     /locus_tag="PL000_07130"
     CDS             complement(7286..7972)
                     /locus_tag="PL000_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88706.1"
                     /translation="MIKKLFLLLLAPLCVLVGSAYANQRIAAVAVINEDCQDLVQWIE
                     RNERIGIGTFFLYPCGEVESYLPLLESYIEEGVVQILDNPFQMAGQDIDHPMHRHMAF
                     EQALAACFYYSVLYCDEEAYFDWLIMLEQHDLPNIPTRPLNVSLDLVPQQVSVIRLDW
                     FRMGENSFPAIFFDDDYIDDHEDDLKDAIFGSRSWNVRHKFAVRTQEMPKYFPKKKPK
                     EPKPKHPFPR"
     gene            complement(8708..9028)
                     /locus_tag="PL000_07135"
     CDS             complement(8708..9028)
                     /locus_tag="PL000_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88707.1"
                     /translation="MEINSTPSPEGAPDDTIEVESIYWQPYTDENRPADKSKCLYMIF
                     TEMEGIYDAADAHFIPDMSRVPNSHIAIFRELKDAGCTGEESSGENPEIEGKHSTKEA
                     SEEV"
     gene            complement(9028..9327)
                     /locus_tag="PL000_07140"
     CDS             complement(9028..9327)
                     /locus_tag="PL000_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88708.1"
                     /translation="MKCEFLGFDSCEYGQMIGVASVRLDGNIIIKYKVTMRKDGKNFF
                     ISVPSIKISAPTDPAEYISCVLMDRMTEELIDKCIRDNLKDIVTLKSDNYNEVPF"
     gene            complement(9353..9976)
                     /locus_tag="PL000_07145"
     CDS             complement(9353..9976)
                     /locus_tag="PL000_07145"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HNC88709.1"
                     /translation="MDTDGRYLHLFFDEPPIPARGVRFSNSSNSSFAYNPDTKQKERL
                     KLLAYWQLPKDGATFFPVGPLFFFMLAKFELPKSLSKKRKQELLGQPHTHKPDCDNLE
                     KIYFDALKGLLYKDDAQIAMSLTRKVWWKDSGVSIFVGNIDVYYEVLMDHFMMCTMCK
                     KIIPVNEIGICLGCQTGTPNLYEEMLLNNEIHENENRPVYPYRAYDE"
     gene            complement(10030..10590)
                     /locus_tag="PL000_07150"
     CDS             complement(10030..10590)
                     /locus_tag="PL000_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC88710.1"
                     /translation="MESQKPNERTNPSVNNKKMGRPAGTYTHIPHYPGFVTVREAAEI
                     LKIQESSVRMLIHTGRFQRYTVEGKRWVYLKKDDILDHKLWKYSRVHAAIKQAGIKDA
                     YSVEETALILSDLLQLHIDQTVIRHYINKGMLPAYRLGRYIAVKYAHIVELKKSIEEE
                     MRWREKQKAAVLERMAQADQSNTKFG"
     gene            10712..11278
                     /locus_tag="PL000_07155"
     CDS             10712..11278
                     /locus_tag="PL000_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC88711.1"
                     /translation="MPQLITHQEKNYIRISSIGDKGHYDNIHPHILDRAKMRGSQVHQ
                     AIEDELMGTFGLCEPIWMGYVESWRKWKSHTDFVPYKIEERYFNDELMITGKIDLIAD
                     IDGIKYLFDWKTASTRSSQWEIQQGLYALLLREAQIELSPYLRVIQLSKDGTLPKVHT
                     FEMNDSLEKRCLREVKIAWDKVRKREEI"
     gene            11595..12173
                     /locus_tag="PL000_07160"
     CDS             11595..12173
                     /locus_tag="PL000_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88712.1"
                     /translation="MSNKISPISSYVPYGLEDQFSPDLLEFLRGLESITSSMHTFAST
                     TEIKDQGIAQASVDMWADARTIQETLEVARKKALQKYEAEVKKINALAELLEKPLDEI
                     QQIFSRKLSDFVVEFEEVNNLLGTDADKIKGTKGALYQKTTESYEVEDIEKVPDEYFM
                     LDEKKLKKVVGAGVVIPGIKPKKVKSLIMRRI"
     gene            12173..13060
                     /locus_tag="PL000_07165"
     CDS             12173..13060
                     /locus_tag="PL000_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88713.1"
                     /translation="MALPNTHSLQTIDDLKKNLEVMDRLSGIALSTRLFPNYTKEQLT
                     LLLLTAAELGISPMRALNGGLVIVKGKVSMSATMQCERIRQAGHSIKTEKCTDHECIL
                     VGKRKDTGDEMRVNFTIQQAHRAGLTKNPTWLAYPQIMLQHRCVSLLARFLFADIFGS
                     IYCMDEGREIAEAEGVEFPIAIDDQIAEIAEEAQVLESNVSQDVQDSGDFLHDIQEPQ
                     QEPQPAPETLLGEFADELHGSHFSYTDKEKVLDYLRHLFAIKKMEISLFIEALRASIS
                     TPAGQESLKRLFDNWAEKA"
     gene            complement(13071..13859)
                     /locus_tag="PL000_07170"
     CDS             complement(13071..13859)
                     /locus_tag="PL000_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88714.1"
                     /translation="MFFSGHHRILNHAQRNLDSICHLLYDAPFISGRRWDFEDIPGSS
                     NQDEWTNDQEIACIPQVFTKEKSSKKKTAKKKQLDTRAELILPDRDLFSILDEEKKFF
                     SSRTSDTDEHVKQCTQAQFKTMSVTTRHQKLKIRALENYKITKRITHDEFLDMLGLCL
                     KRHVPTDPEIAFMDYLLGLYGIDDYCTVAFKTPWTKKFLKSRALEDGEAHIVPDEIEE
                     KINTASEIKSKLKYEEELSRWKQKQTQPLFLESAPNRKAAKLRP"
     gene            complement(14029..14283)
                     /locus_tag="PL000_07175"
     CDS             complement(14029..14283)
                     /locus_tag="PL000_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88715.1"
                     /translation="MRYGYGHEAPSEWGYDGYESELGILREDLEKEILGKREECQQCV
                     QSAVDLLFDEQPLKLSALEQVKRDLAYMASFYGIEVPRAV"
     gene            15252..15773
                     /locus_tag="PL000_07180"
     CDS             15252..15773
                     /locus_tag="PL000_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88716.1"
                     /translation="MNSKEEDRAYHETLANIEMPSNILLSLRERVAIFERCNRSLEMF
                     GELCSRYSSWKERARERPKNDFGVMYAWKLDNMEREMSEKKLDDQRFAENIEFAKRAY
                     RLCPKSGSGWTVDMSLAVEEPYFELRSGSYSKTFLIKEPRFVDELGEMLERAGLATKS
                     EPQYYHQDDRKHA"
     gene            15997..16260
                     /locus_tag="PL000_07185"
     CDS             15997..16260
                     /locus_tag="PL000_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88717.1"
                     /translation="MSKKPSVEEQFTVDEDKRAKLVAHLLDQLEEEAPPEDNKKRSNF
                     KISVAELFLTMVIHEEVDPQSYIKLIDRIIERVHKHLESLNKT"
     gene            17248..17538
                     /locus_tag="PL000_07190"
     CDS             17248..17538
                     /locus_tag="PL000_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88718.1"
                     /translation="MTEEEMSYEKTERVEKLFRDTAQELSNIISYYLKKTGTTHEGTA
                     LLFQLQVFRCLVADTIYRIVPPHLCDQVVDGLADDIKKEIKFLVENKGTKDD"
     gene            17531..17926
                     /locus_tag="PL000_07195"
     CDS             17531..17926
                     /locus_tag="PL000_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88719.1"
                     /translation="MTEGESSHPITIEPTVLTYCQNVLHSIAGWPEFVDSKYECDRHI
                     FLSNLGIPDMQWDVDNQSFNEALKIMGHALKGIYDTYEKRYDFSEPELTFCSMFSYFT
                     IKVGMYKKNKQRVLEIAGLVKLCDPEEDE"
     gene            17919..18179
                     /locus_tag="PL000_07200"
     CDS             17919..18179
                     /locus_tag="PL000_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88720.1"
                     /translation="MSDYPSKKYKTVHVSKKDFIDQLRAVFLTLPSEHIASHLAETLL
                     IIQKMTVKDLLRPEVKKELEEDIERYSDFVTEIIRELETTYD"
     gene            18316..18645
                     /locus_tag="PL000_07205"
     CDS             18316..18645
                     /locus_tag="PL000_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88721.1"
                     /translation="MNFPSIKQRGIREVVFKRSFRRLIERKNKMDSSAKKALSQITRI
                     LENSDLDFPQKNYVITELFVQMVMEWGIDDYAVDDLFDYMYEGVVDARLKFKKAQATS
                     SIGLEND"
     gene            18638..18904
                     /locus_tag="PL000_07210"
     CDS             18638..18904
                     /locus_tag="PL000_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88722.1"
                     /translation="MTDPAYLHRSIFLDVSGDELVEYIAEGFLDKGAENLALSLAKIM
                     VEIYMTVDNELLGDAKLESLDKILAEYANFVKKSALDLIARKDA"
     gene            complement(18917..19738)
                     /locus_tag="PL000_07215"
     CDS             complement(18917..19738)
                     /locus_tag="PL000_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88723.1"
                     /translation="MANIYEERLGKILDFKHYREIAEALRSAKSGREDSIFKSMGQQR
                     EYYCSHFRKFKRLKFFICIPQIIALYDLKCAGASVKEIIGVLPKNKLLRILEVGEWIT
                     KNYRPSVDTYPLLDPRDFSIDQAKSWIIRELKDRDIPPQIHNALLSLLNALDHGVWTR
                     GSGVKGGLGLPRRFLVTHLNRILCDELGGRLDAETPQDLTALLGRMRENHEKTLLAQE
                     PESPIKNLPASPPYERERSSYLVSNALEDLFRELGEIKAELRSINEKLKRLRLAN"
     gene            complement(19731..20429)
                     /locus_tag="PL000_07220"
     CDS             complement(19731..20429)
                     /locus_tag="PL000_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNC88724.1"
                     /translation="MEDIQEEVQIYVTKDYARFSLSPTNRDICAKHVTRLVRSISERN
                     LLSFNPIIVDKDLCVIDGQHRLKAAEILQIPIYYRIISNASNDCARLMSSNSKTWTLW
                     DTLKYYSQIGAEAYVQWKQLVDDHPLLKTTVLAIACVNSQDRVVFREGRVNPPEMAEV
                     TYILNGIEAVRSKLRDLNTRFSENDLRFMISIFKRLRSEGFREEDVKKRIRNIVPEMV
                     LYRDSVKIYEVLHG"
     gene            20704..21168
                     /locus_tag="PL000_07225"
     CDS             20704..21168
                     /locus_tag="PL000_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88725.1"
                     /translation="MAEPKKEKKRPGRPRKPVPAHYLDPSIRRPGPAPKDVDKELFES
                     LCGIWCTIMEIESIMKTDAATIDKWCFRTYGVGFSSAYERLCNSGKASLRRNQLNLSS
                     TNAAMAIWLGKQKLGQSDTPAESAMIEGLLAKYVATLKQGAENTLKRSEIDD"
     gene            21168..22415
                     /locus_tag="PL000_07230"
     CDS             21168..22415
                     /locus_tag="PL000_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HNC88726.1"
                     /translation="MQPMSVKQCHALIESDARLNVFEGPVRAGKSFVSLVRWIDFCLN
                     GPPGAMIVVGRTDKTIKRNIIVPLQELAGKIVQYSIGKGELRVGSRLIYVVGANDDRA
                     EAKIRGSEFAGALIDEATLIPESFFKMLLSRLSIPGAKLFCSTNPDSPYHWLKTDFID
                     RAKELDMKVFSFDIHDNPSLTPEYIENISKEYKGLWYKRYIEGKWVMAEGAVFDFFDE
                     RVHVIEHPPGVPDGYIVGIDYGTTNPCAFVLIGYSGSTYPNMWLEKEYYYDSRKELRQ
                     KSDYEYVDDLCAFLDGYNPKAIYIDPAAASLKQEMLRAGIGRIVDAKNDVLPGIRYLG
                     QLLSAGTYKICANCHNSIKEYANYLWDSKASLKGKDVPIKASDHAIDAQRYALFTHFF
                     GTSTKGMTEADALEMEQRYFSSI"
     gene            22691..22873
                     /locus_tag="PL000_07235"
     CDS             22691..22873
                     /locus_tag="PL000_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88727.1"
                     /translation="MAKKWIQGAIEHPGVLHKELGVKPGKKIPESKLKKAEHSKNPTL
                     KKRAVLAETLKSFKKK"
     gene            23250..25310
                     /locus_tag="PL000_07240"
     CDS             23250..25310
                     /locus_tag="PL000_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88728.1"
                     /translation="MDGISIVNEFNDFYEEAYYAWNPYYPLADADIRAYLGDQWTEQE
                     KQKLFQEGRSNWSFNLIRKNINLVDGYQRSHRLSSIVLPQHPKDQEGADELSDLVQKV
                     FSSGDGYKHLSDAFSGGLKTAWNLATVWMDYRDDPVDGDIRFGREPYSGFITDPYLTQ
                     LDFSDCSYVLRRKYMSPERAASLLPEQADEVFAVHTEGWSRDDKFTWLPYQQQPNGQD
                     FIAYNEFYKQMWRQVPVLVDTQTGGAWDIKLDKTSLGEVKRFVKNNPTYALIYRSKRF
                     IECHVILNNVYFKTMENQFGLDEYPFTPVAAVFEPESEFWGLKLQSLVRVCQNPQRLL
                     NQFLVQMDDIVKSQVNSGWIAEEDSVVNPQSLFQTSQGRVIWKKKGASPAALERLQAA
                     LIPESFFQMVDIFSKGISEIMGVNDAAFGIPESGNESGVMMMLRQGAAISNLQSVFDN
                     LRYAQKQLTRKVIKLIQTWSPGKVERMLGRKPTEQFYSKEFVKYDISIQEGMMTDTQR
                     QMHFRQLVDLKQLGAPVTGAMLAKAAPIQGASSYLKELEQSEQQQAQQAQQVQQVQAQ
                     LLEGQMQLQKASAVEKMAGAKERFTRSVANVGLSDERAARAVDDRASAALDRAKAIKE
                     LEAIDDERIIKYLDIVHMMEKLNQSKEKEIKSDDLAIAAEGQTLGEIQASTMSRELTG
                     KEPV"
     gene            25827..26081
                     /locus_tag="PL000_07245"
     CDS             25827..26081
                     /locus_tag="PL000_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88729.1"
                     /translation="MGQIFIGQLSTLAMDNVNNQPPHEVNKAQEEQTKEDDVDISATS
                     KSKTQQLEEMMGQQALGPMAQGNQNINPTQEVENGTPKPF"
     gene            26128..26388
                     /locus_tag="PL000_07250"
     CDS             26128..26388
                     /locus_tag="PL000_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88730.1"
                     /translation="MKESYASRRHESEGMRHEMKEDRRMERREESRERSGMGRDFNLK
                     DNEQAWMVKPIPTDSENYDLSRIKPYNDGSKGYPAEAWNYRY"
     gene            26483..26854
                     /locus_tag="PL000_07255"
     CDS             26483..26854
                     /locus_tag="PL000_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88731.1"
                     /translation="MAQETGETRDAIIEESNKRLQDIIYANRHRQEKYWVVLYAKPTR
                     NTVNGFPALAQHIKAYGEKPKSQVGMVIGEVDPLKSTISWEVNMPQAPFDFDALRALG
                     AKAGDTVVTETTTIPGAYVTQ"
     gene            26884..27528
                     /locus_tag="PL000_07260"
     CDS             26884..27528
                     /locus_tag="PL000_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88732.1"
                     /translation="MEDIQTGVTQEAAALTDSQPLEPAEQVHQVPLAALESERSKRQQ
                     YEEENRLMREHLALLQRQSQVQQAPRDDFGGLQDSDVMTVGDFKKMATGMARQMESSI
                     EELKMQQKNPDYSSVVTKYLPGVLKENPRIAEHLKQTQDYELAYYLAKNSDEYKSEHK
                     RAQRSAEAERIVKNAEHSSTLSGVGNTVPVAGTKTYKSMSDSEFRELMSRNLGY"
     gene            27553..27927
                     /locus_tag="PL000_07265"
     CDS             27553..27927
                     /locus_tag="PL000_07265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HNC88733.1"
                     /translation="MSNMTTVAVLPPAVRDYYDRLLLMTAYPTLIYTKFAQKRVLPEK
                     MGDRIVFRRYARLPTVPIPLVDGITPPGSPLSVTDIKAQVAFYGNFVTITNQVQLTVE
                     DRVNIQALYKLDLIDLELLNAA"
     gene            27917..28597
                     /locus_tag="PL000_07270"
     CDS             27917..28597
                     /locus_tag="PL000_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HNC88734.1"
                     /translation="MQLKDNKGEGRKATLNDLTIEPHKGMRKSELRIYMKSQREIRRG
                     FPAWKQVTKVTDCLNESSRLLAQNLAQTMDQVTRDCLASTSSVQLCQNGINGNTPTEL
                     TKQDIDSAVKTLLGNDAEMISEVVIGTNQISTTPIRPAFWGYIDTDLLSDLEAVSNFV
                     SSSQYPGNQNIVLDSEWGATGNVRWLYTSVGSVSSAATPVYNNFIVGRQFADVKSSLI
                     DLELLEAA"
     gene            28653..28937
                     /locus_tag="PL000_07275"
     CDS             28653..28937
                     /locus_tag="PL000_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88735.1"
                     /translation="MKIRSDSLNSAVTQRGRSEEVFPPRKLGHKSNRLKEAYAVVNLR
                     SETGDFYVKPLGSAGAADPLNQRGSVGWQHPFTARILNDAFMLNLMATAS"
     gene            28948..29544
                     /locus_tag="PL000_07280"
     CDS             28948..29544
                     /locus_tag="PL000_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88736.1"
                     /translation="MAQIYTTSWTNPATAVAKNVSVGFEVVRVQTIDVTNGGSWMWVY
                     GMPNGYYINVATGAVTTSNGWTPLAENTLFAAPITAVTKGADTVFTCSYLDQFSFAVG
                     DTVFATAMADDLTGTTLNGTYTVKTVSATQITCEESTASGYSVYVSGGFLSQVSDADG
                     KPYPQANVAIEGGTVGTGMVGANSAAMVAIFEGSNPVV"
     gene            29617..29985
                     /locus_tag="PL000_07285"
     CDS             29617..29985
                     /locus_tag="PL000_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88737.1"
                     /translation="MTKVLNESMSNEALNALPIVPIDGRQPANEKEEKYLREIGVYEF
                     QNLEDPGLQITFPYGGTKKSQKFILMHGGRYKLPRHVANWIESRGEPIYKWKPNGEGR
                     MESTYSGRKPRFSLRPVFGS"
     gene            29988..30884
                     /locus_tag="PL000_07290"
     CDS             29988..30884
                     /locus_tag="PL000_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88738.1"
                     /translation="MAATWTLSQIQTKVRQLIGRFSSAEISNAELITRINQYYTLTFP
                     AEVKLETKHVYYQFTTSANQPYYDIPQTTYTNFEPPATCNNLLMYWFQDPAYFFQANP
                     LQYTFLTPWTGDGTTTAFNTTITGFPIYPGTTTIYDGVELFEDTNQTYTTSNVTVTGS
                     LGGSMVLNYSTGVISVTFNTAPANGQNINLNYVVFAAQRPQAILLYNNQFQLWPVPDQ
                     AYIIKMRAWGVVDALVNATDTPQLNEWGPTIAFGTARQIAADYGEMDAYAQLTVLYKE
                     QLGYILTRTMQEMMSSRAPPTF"
     gene            30911..31477
                     /locus_tag="PL000_07295"
     CDS             30911..31477
                     /locus_tag="PL000_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88739.1"
                     /translation="MAWDITQPTDNTKIRDLGVVIRPNWQAIQTADSSFTPDAMNLTN
                     RTVAALPVDPTAIADAYVMYCKTDAAGNSELFGIDPNSVISQFTSTDYTLAQDGYAVI
                     PPGILIQWGRDLMVGGTSTLAVSFGKAFSSTAWVVVSTPYDNPITGSTPREWGVNNIT
                     AAGFTANSFNGVVPGGGVNFGWFAIGPM"
     gene            31485..33380
                     /locus_tag="PL000_07300"
     CDS             31485..33380
                     /locus_tag="PL000_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88740.1"
                     /translation="MTQPQSSGLVPVLLAPFSTGLVNNREPWLMPADAFQEVDNFNIH
                     HGWLEKRQGYRFWGNLGSSLRVMGLVYYLENDGSKTNLAFDTKYGYIYNTLTNVYDDL
                     DSGAQTLDGGEYDYIWSVNWQSSNIDNRLYYTNGLPYDGTAYNGIRFYPKDAAHLTDT
                     DLLKPSLGGGLKLYGAKLLFTIGQRLVCLYTYENNGASTSSYPQRARWCARQDPSNWD
                     DTVGGGGAYADAATGDQIISAQALQNQIIVFFTNSVWTLTPTSDPNKAFKWSRLNNFR
                     ACDGKMASVAYDRYSTAVGIRGITSTDATETRRSDTQIPDFAVDEINFAEFKKVFCFR
                     DYASLRWWTLYASEDSTENNSALIYDDESQAYTTYSIALNCLGFGNSSLDYKLSDFTS
                     ANGLDFSLNDMSEETLQSFYLEAATDTLLGGTTNGFVYQLDISSQDAGTDFGASFTTA
                     AWNPFQIQGLESQMSYVDFYVDTDPFTLGTVEFYKNDQVSPYASQPLVFLPNLDYLTS
                     IAQIDKTSPCAVNAPQHGLTTGDVVFIYGVQGMVQVSGEYTVTVVDENNITLDGINAT
                     GYTTYVGGGALYRREFYQTKTWIRAYGGGIGYLHWIKVTINEGKTPMRFHAIKPYFRP
                     IGTRTIN"
     gene            33402..33536
                     /locus_tag="PL000_07305"
     CDS             33402..33536
                     /locus_tag="PL000_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88741.1"
                     /translation="MPFKSKAQRRWMYANDPKMAAEWQEETPKGKKLPEKVKKKKPKK
                     "
     gene            34084..34650
                     /locus_tag="PL000_07310"
     CDS             34084..34650
                     /locus_tag="PL000_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88742.1"
                     /translation="MKKIDELRWVRAFSPDVIPKYLIEQIKKRDFEVDDFLRYHKVAC
                     MRETQDGFTLNPLSQLWVLANPDNLVKGVLWFVVDPLCKAIVIQTYSVDPEYWKDGGA
                     MEKCVSHVKDIYRKTGMKKIYWITDYPKHNKRHGFHPSKSILMEYREEAPEDLKDPEE
                     IEDGQNTYGGREPQRERTTPDTPATTEL"
     gene            34571..35074
                     /locus_tag="PL000_07315"
     CDS             34571..35074
                     /locus_tag="PL000_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88743.1"
                     /translation="MGKTLMGGASHSGNAQLLTPQQQQSFNSLLSALGGLGQQAYSDF
                     LQPYSPQQYQDFYRQSFIEPAQQALKQDIIPALKESMLGLDESGSSDLNRALSRASVD
                     LSSLLGQGILNQYNQSNANRLSALSGYSGLLGAKTFEPVISQSSGILGPLIGLGGQLG
                     APSLMRR"
     gene            35078..>35946
                     /locus_tag="PL000_07320"
     CDS             35078..>35946
                     /locus_tag="PL000_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88744.1"
                     /translation="MAIVVNKDAGLIQGLQTAGNALAATLTHNALMRAQENQQKKQQA
                     QYAQLASKLQGKPIDQNLLFSLLAEPGGMEALKTLGPLLGGVLKENARSAGAGNWLQN
                     AVLQATGGHPQNPQNPEAPQMPYGINQGVQSAGQGILDSVMGSLTPIDQPMNDGYHLN
                     KDFYERNGLAKPDISDLQEWRSGTLNAPAVSGVPSAPPLPDENRVGPREQTKFQMGGQ
                     SGMDLHPPQENFRQVPIKQPIPQISDLNADQQEKLAQILSGSPYQEHRDLAKNIQDQV
                     KQNTRNFHEDRKY"
BASE COUNT        10143 a         8436 c         8424 g         8943 t
ORIGIN      
        1 caaaaccaat ggtttgaacg acgagcctcc agtatgtttg ctcaaatagg acaatctaag
       61 gaagcaaacc tcaccgccca agagatgctt gagggagaga tgctgctgga tgaggcttac
      121 ctgaatgctt atgacaagat cgcagacgaa gatgtaaaaa aatatggata tgtgaaaggc
      181 gacgttgaga aaagggcgcg agactccatc aaggatgatg agaccaaaat atttgaccgc
      241 agcgtctata ggatgcgtca gctagaagaa aaagaaatgg gcgataggaa gctacaggcc
      301 aatgtaggca aaggtgtaga atcaggaacg ccccttactt tagaaatggc acggaattat
      361 attaagaaat atggctctac agagaaagct cttgagatcg ccaagaaaaa tggctacaca
      421 gtacctacta aatccgaact agaattctat caaatgtctc ctcagcaagt aagggcgtat
      481 tatggcagcc gctaaatctc aaaaagagtt tgaagaacag ctagctttta tggatctcgc
      541 gggagaagac gaatccaatg aatcgggtga tgtgtcctct agaaattcct cagaggtggc
      601 ccaacccagt ctccttaatg atattaaaaa ttatggtaaa aatgctgcca aggctctggc
      661 tattggaact catcggttag gtcgcgcttt gggcccgact tttgaaggtg atttgtccac
      721 aaagggagaa aaaggggtcg aacgggattt cagaagttac ctggacgaag tatttccttc
      781 tgaacctggt ggtgctgtta cagaaactac ggaacgattt ctccagaatg cccctaccta
      841 tcttgcaagt cctggcgcat ctgtgggcgg tactctggcc agaacggccg cgggtgctag
      901 tgctgctcaa ggtgtcaaag agttgggtgg tggagagact gcacaagaat ttgccgatct
      961 actattttat ctaacccccg atattgggaa aaaaataatt ggatctaaga aaagtcaaga
     1021 gtggatacga tgggcacgcg gaaaaggact gtcagataaa gagatagcgc ctctcatcag
     1081 agacggcaag ttgggaaatc tcttctccca cttctccaaa aaaggccaac gatctagaaa
     1141 cgccctccag aatacaaaag gggcgataag tgacctttat tcatctatga ggtcttctcc
     1201 agaggggact aaagttctaa gtaagccaga cgaactcttt gtaaggctca aggaagatat
     1261 tagtcatatg ccaagccatg tgcgtaaggc tattaatgat gatgttaaca agctgattgc
     1321 caaaccttct cctacaaatg ctgatcttct ggaattctgg caagacgtca atgctgctgt
     1381 cgcgacagat cctgaaaatt tgcgtagact ggtgaacgta aaggagccta tttataggca
     1441 tttattagaa aactctcctc agttagctaa agattttgag atgacaaatg cgctttatgg
     1501 aaagtattca aatgccgcat ctaagcttaa atctggaata aaagatcaac taatcgatgc
     1561 aggagaacat ggcgccttct tatatgggct gactacggga aatcttccct tgctgatttc
     1621 tgtagtagga gagggtgcag cacgccgttt ggccactgag atgttaatca atccgaggtt
     1681 ccagcagtta atacccaaga ccgtaagtgc gctcaaccag aataaagttt cagtgctaaa
     1741 acaccttgta aatcttattc aagatgagat catagaggaa gctcccgagg cagccaaaga
     1801 gttggcatca atcgattggg atcagtgggg agcgggagcc tcataattaa ccgtcgatcc
     1861 attctaggaa tccccatcct actgcggcaa gcactcccat aaaaaagtaa ctcatctcct
     1921 acctccatac tggtatgatt tcgtgctacc ataagtataa cattatcatg cttttacatc
     1981 aaccatcttg ctgctggaga aataaacccc tatctgcata atagccataa aagtttttac
     2041 ttaacttagg tgctttatgg ccacgccttc taccctgcat gcaatgggac tcgaaaaacc
     2101 gctaattcct gttttcccct ttcctctgta tatggatagg gcgcctacaa gctcagataa
     2161 aaactttaat attggccaaa tctggttcta caagattgac cccgacacct gcgaactcta
     2221 tgcctttgcc ggactcgata gcacccagtc ggccatctgg ttaacaggaa gtgttggccc
     2281 agcggttctc agctctctag aagctgaagc tggtgatact tccgtctctg ggtcatcggg
     2341 taccatattg ctggctggga cagcaaacca gatcgtcact actggcgaca atacagccca
     2401 tagtattacc ttttctctgg atccagatgt tgttatcacc acttcgctag atgttccact
     2461 gattgttcct actggcggtg atttatccgt acagcttggc gacaatatcg gagctaatta
     2521 tctggcagtc gaaaacgcat taggctctag aaaaattatt ttagattctg ctggcgtaat
     2581 cacagcccct cagatgcaaa ccactactga cctcactctt aagctgagtg acggagcggg
     2641 cgcctcgtct ctctatgtag tagatgacac cgatgtacaa aaatttaaag tcatatctga
     2701 tggacaagtg gaagtctcta ataggattgt agccgcttcg ggtaacttag aagtcgctgg
     2761 cgcagatgtg attaccaccc tcggcgactc aggtggcgtt aataaactct cgatacaaac
     2821 cgtcgcatcg gtcgaagtag catcgatagg ttcagacggt agaataacgg gatctctcta
     2881 tagtttcact tcaacctatg gaatgagtgg ccaagttacg ttagtggctg gtactgcaac
     2941 cgtagttaac gcaaacattg ctaccaccga tgtcgtgatt ctggaaagga ctgatatcaa
     3001 tggttcaact gcattaggag ttccagtagt cgtcaaaaac gcaggtgtca actttgtaat
     3061 cacagcagta caagctggca caccagcagc cactgagacc aatgacgtgt ctatattcaa
     3121 ctacgtcatt atgcgcaggg cataaagatg agcttacttt ctaaatgtga gctaagggtg
     3181 ttcgactcgg catctatttc tggctcctgg cagacgtttg gagcagtaat tggagacccc
     3241 gcctatcaag tggtggtctc caatgagtcc gacgtcagcg tccagatagg catagacaat
     3301 agcacagcga tcatccgtgt atctcctgga caagtacttc ctcttacgta ttacagccgc
     3361 cacaataccc aaaacttggg ctcgtacctc ttcaaaaagg gcacccagtt ttatatcaag
     3421 caagtatcgg cagctggaac gggggatatc attctcaata tcttagaaac ccaggcgcct
     3481 tcatgagtag ctccacagcc ccttgggata atcggccttt caagttcggt agtcgcgatc
     3541 ggcgagagtt catcgtttcg gacacagaga tcgccttacg ggctatcaat gacaccaacg
     3601 gcaacccaat ttttctggcg tgggcaaagg cgggatcagc ggaatctagt gccatatggc
     3661 agctgcgaaa gattcaatac gattccaatc aaggcgtcac cagcgtaaca tgggcggaaa
     3721 acccagatgg gatacccact acgaactatg aatttgtcta tgcttcgacc tctgatctca
     3781 ctgttacggg tgttacaaat gctaatcccg ccgttgtgac cgtttcctcc atagggaccc
     3841 ttcaaaatgg agacctcgtt atcttccaag gtatctctgg aatgacagaa ctcaatttcg
     3901 atggctccaa catctatacc gtggcgaaca tcgcgggatc taccttcgag ctttcgggta
     3961 tcgactcgag tgcctatgga gtctatgtga gcggaggaac agccaatttc tccgaggtcg
     4021 tgaactatac gtatgggtga ccatggctta taagctcaat ccctttacgg gcaacttaga
     4081 tcgtgtggac gctggtggaa gttcaagcgt caccataacc tttgatgcag atagcggcag
     4141 tgctaccacc ctctcaaaca ctgttaacct tctatcggac gtcacggcat catctggtac
     4201 tccttttgag tccacggcat catcacatac cctcacttat acgttgcaaa ggtcggcctc
     4261 tacttctacg tcagatgcta ctctcgttgg tctagcagcc tttgattctg ccgctttcga
     4321 tgtagatgcc aatggcttcg tgcagcttaa cgggggtggt atagctacta cccaatatgc
     4381 tccaaattcc ggtaccaatc ccgtagtgcc tacagcagct ggcttactta acatagtggg
     4441 gacgggaagt actacggtag tgggaagctt aaatacggtc actatcgaac tcacaggtct
     4501 taccgatcac aatgtcctgg taggcgctgg aacgtctaca gttaccaacg ttgttccctc
     4561 tgctacatca ggagtacctt tgatttccaa tggtgcgtcg gctgacccct cttttgggac
     4621 ggcagttgtt gctggtgggg gtacaggctc tactagcttt acagcctatg ctcctgtgtg
     4681 tggaggaact acttccacaa acccacttca aagtgcatct actggacttt ctacctctgg
     4741 atttgtcctt acaagtaatg gggcatccgc attgccttcc ttccagagcg tatctgcatc
     4801 tggggccatc actaccatca cgggaaatac ggggggagct gaatctcctt cttctggcaa
     4861 cttcaatatc gtagggactg gaagtataac agtagcgggc tctgcaaata cagagactgt
     4921 ccaacttacg gggcttacta accattcgct cctggtggga gcaggcacag ctacaattac
     4981 caatcttgga gcagctacta acgggcagct tcccataggg tctacgggcg ctgatccagt
     5041 tctagccact attactgcat ctacgggtgt ctcgataacc aacggggcag gctccatcac
     5101 gattgctgct accggagcag ggatggccac tgtagaagtt actgggacat ctcaatcgat
     5161 ggctgtagac actttctaca ttgccaacaa cgctgggtta gttactctca cactccctac
     5221 gactagcgct ttaggatcgg ttatagagat tgccggaaaa ggtgcaggtg ggtggaagat
     5281 agctcaaaac tctggacaac agatctacct tgggtctact gctactacct ctgggatagg
     5341 cggttcattg gcctcttcta actttgcaga tacgatcaag ataagatgta tcacggctaa
     5401 tactttatgg acagtggtag gtgcccctgt aggcaacatc acatacgtat aataggtaac
     5461 aatggtcaca actaacggca tcaacaacta cactcctgag aactttcagg taggcactct
     5521 atctgtaaac accaacgctc agactggtat tcttctcagc aagatcacgg tgaaaggcgc
     5581 tggtgggtcg tccgattgct atattagtgt cgatgcaaat agctccacag cgcatacggg
     5641 atacggattt gtaaactcgg gggacagtac ttattggtca tggtatcgag agaacacttc
     5701 taacgtgatg aagttagact atcaggcggt taacaaatgg caggtagatt cctcaggtaa
     5761 tgtcactgtt gctaatggtc ttatcatcgg atcgggaagt acccttagct catatgtgac
     5821 gggatcttgg actccctcta tctctttcgg cactggaaca acaggcataa cttacagcac
     5881 ccaagcaggt acttatacgc ggattggcga tttcgttacg gcatatttct acattgtatt
     5941 gacccacaaa ggaagcgata ccggcaacgt tcgtgtcaat ggacttccct acactgtttc
     6001 aagtggcaac aatgctgggg ctgtagcgac ttcgaacatt acctacactg gtacctacat
     6061 catttgccaa ggtatcgcta gccagactta tggcaactta caatattgca cgaccgcagc
     6121 cgctggggct cttctcacca actccaactt tgctaacaat accaagatcc agggatcatt
     6181 catctataag gtctaatatg gcactcacta aatgcacaaa aacaagatat gagatcgagc
     6241 tgaatgaaaa aggggagatc ataaactgtt tccaagagga caagaactgt atcgccgaaa
     6301 atggcgtaga aattgcctct tataaccacc gagcacccat agatttttac gcgcttcaga
     6361 aatccctcag cgttgccaaa gtctcttctc tcgttgacgc tatcatttag gagattgtat
     6421 ggatttaaaa gaattgcaaa aagatcttca tgaacattta gatgtgttca aaaaatctta
     6481 tgaagacatg gttgaagttc taaaagagaa gatttgtaac atcatatgtc atgatctccc
     6541 ccgagatgat gagcctaaag atccccccgc ggctccatag gtaaccgatg ggtattttac
     6601 tgataggtgc cttggtgacc gtcgttgtca tgggaggagt aggatggtgg aaaagtatgc
     6661 atcatcccaa aagcgagcta gagtccattg ttgaggaagt catcgacgag caaaaaggcg
     6721 tagaaatctc cataacccct aagaaatcgg acgattgaag actatcccat aatgtgtaca
     6781 caagggaaaa gtcgatgctt gaagatacgg aagaccttct ctacgaagct ctcgagattc
     6841 gtaacgagat ggcgaaattt ctggaagacc ttggagcgga tgccgtagga gcacttctgg
     6901 gctctaattt cctgtgtatg gacatcttga ttcagtatgg agtgcctctc ggccatttcc
     6961 tggatgctca gcccgatttc tatcgtatgt gcttacgagc aaaacgcctt aatgagctgt
     7021 aagaagccca ctatttctag ggggacaaca actcttaaag ttagcggtta acgctataga
     7081 catcggatcc gcttccgctt gggaaagtga cggttccatc agtgttgacg acagaatatt
     7141 cgcccactgg atcgtgattc actgctgcgc ttaagggggc tgcacagacg attccgcaag
     7201 ccattaatcc gccaaaaaat ttaattacgt ttttcactat tttctccggt tttcggtttc
     7261 gttagttcct tctctagcta tttatttatc taggaaaagg atgtttaggt ttcggttctt
     7321 ttggtttttt ctttgggaaa tatttaggca tttcctgagt tctaacggca aatttatgcc
     7381 gcacgttcca gcttcttgat ccaaagatag catctttcag gtcatcttca tgatcatcga
     7441 tgtagtcgtc gtcaaagaag atggcaggga agctgttttc tcccatgcgg aaccagtcga
     7501 gtctgatgac actgacttgt tgggggacca ggtccaaaga cacgttcaaa ggtcttgtcg
     7561 ggatgttggg cagatcatgt tgttcgagca tgatgagcca atcaaaatat gcctcctcat
     7621 cgcaatacag gacggaatag tagaaacaag ctgctagcgc ttgttcaaaa gccatatgtc
     7681 ggtgcattgg atgatcgata tcttgtccag ccatttggaa ggggttatcc agaatctgta
     7741 ccactccctc ttctatataa gactctagga gtggaagata agactccacc tctccacaag
     7801 ggtacaagaa gaaagttccg ataccgatgc gttcgtttct ttcgatccat tgtacaagat
     7861 cttgacagtc ttcgttgatc actgcaacgg cagctatgcg ttgatttgca tacgctgatc
     7921 cgaccagaac acaaagagga gcaagcaaaa gtaaaaacaa ttttttaatc atgacatgtc
     7981 cggagggtat ctatcaggtt taggttcttg gggatgagga ggatgaggaa ctaagtcggg
     8041 aggccagaag cttcccccga atgctagaaa gctggcttcc gctaaattag gtgggatgat
     8101 gcttttgggt ttatcttggc agctgttcag cgcaacgcac agggctccca gtgcaaaaat
     8161 acacggtaat aatcttttca tttgataata cacagggtta aaatagtaat tacgattgta
     8221 atataaaaat acaatctgaa cgtattgatg agtttcatca tattcagctc catgcaatat
     8281 ttttatttag gccaagtaga cccaaaagca ctatagatca ctgccagaag tgactgctcc
     8341 cccactaaat gctgcgcatt atagtggggg cttctaggga tcaacccaaa gactccagcc
     8401 cgagtcttgt gcgagaaagt atctaataaa ggggcaattc atctccacct aaattgcatg
     8461 ctaggctgaa attttagatg gagaattctt gcctccaatg ttaaagggaa agattttctg
     8521 aacaaaaccc cgtgatctca tctctaggag gatccagacc acgggaaacc catagggaca
     8581 ggtttaaaat tttatttaat cacatccaga tcacatacaa gacttgatca ggacaagccc
     8641 aatcattgcc ttaatgacca ggtcagtgat taaaaaccat gaaggcaggt cgagcccaca
     8701 agcgggttta gacttcttca ctggcttctt tggtagaatg tttaccttcg atttctggat
     8761 tttctcctga agattcttct ccagtacacc cggcatcttt aagctctcta aaaatggcga
     8821 tgtgggagtt aggaactctg gacatatctg ggataaagtg ggcgtcggca gcatcgtata
     8881 taccttccat ttctgtaaag atcatgtaaa gacatttgga cttatctgcc ggcctatttt
     8941 catccgtata gggctgccag tagatggatt ccacttctat agtgtcatcg ggagcgcctt
     9001 ctggcgaggg agtcgaatta atttccatta gaatggcacc tcgttgtaat tatcgctctt
     9061 cagcgtaacg atgtctttta aattgtctct gatgcacttg tcgatcagct cttctgtcat
     9121 ccgatccatg agcacgcaag aaatatattc ggccggatct gttggagcag agatcttaat
     9181 cgatgggacg gagataaaaa agttcttgcc gtctttgcgc attgtaactt tatattttat
     9241 tatgatgttg ccatctagcc ggacagatgc tacgccgatc atttgtccgt attcgcagct
     9301 atcaaaaccc agaaattcac atttcatttt ttaacccttt ttcaatttaa ctttattcgt
     9361 cataagctcg gtaaggatat acaggtcggt tttcgttttc gtggatttcg ttgttcagta
     9421 gcatctcttc gtagagatta ggagttccgg tctgacaccc gaggcatatg ccgatctcat
     9481 tgacggggat gatctttttg cacatcgtgc acatcatgaa atgatccata aggacctcgt
     9541 aatagacatc gatattgccc acaaaaatag aaaccccaga atctttccac cacaccttcc
     9601 ttgtcaggct catagcgatc tgggcgtcgt ctttgtagag aaggcctttt agggcgtcga
     9661 aatagatctt ttctaaattg tcgcagtcag gcttatgagt atggggttgc cctagtagct
     9721 cttgtttgcg ttttttagag agggacttgg ggagctcgaa cttagcgagc atgaaaaaga
     9781 acagtggtcc tacagggaaa aaggtcgccc catctttagg cagttgccaa tacgcaagca
     9841 gcttcagacg ttctttttgt tttgtatctg ggttgtaggc gaaggagcta ttggaagaat
     9901 tagaaaaacg aaccccacgt gcaggtatcg ggggctcatc aaagaagagg tgtaagtaac
     9961 ggccgtcggt gtccaagatg tcacgtctaa agataaaaaa ctatctatag acgtggcaag
    10021 attaagatgt caaccgaatt ttgtatttga ctgatcggct tgagccatgc gctccaggac
    10081 agcggccttt tgcttttctc tccatcgcat ttcctcttct atggatttct tgagctcgac
    10141 gatgtgagcg tattttacgg cgatatagcg ccctagacga taagccggta gcatgccttt
    10201 gttgatatag tgcctgatga ccgtctggtc tatatgtaac tgtaggagat ccgataggat
    10261 gagagcggtt tcttctacgg agtacgcatc ttttataccc gcttgtttga tagcagcatg
    10321 cacgcgagag tacttccaca atttgtgatc taatatatca tcctttttaa gatataccca
    10381 tcgttttccc tcgacggtat acctctggaa acgtccggtg tggattaaca tacggacact
    10441 actttcttgg atcttcagga tctcggcagc ttctcgaacg gtgacaaaac cgggatagtg
    10501 gggtatatgt gtgtaggtac cagctggtct gcccattttt ttattgttga ccgatggatt
    10561 agttcgttcg ttcggttttt ggctttccat gttttcgact cttatgtata ttaaacttgt
    10621 gaatttttcc tttcactgta cagagtgatc tcccattata gtaaaagtca agattttaaa
    10681 ccaccaaaac taaaataaaa gcaccccaag tatgccccaa ttaataaccc atcaggaaaa
    10741 aaactatata agaatatctt ctattgggga caaggggcac tatgataaca tccatcccca
    10801 tattttagac agggcaaaga tgcgtggatc tcaagtccac caggccatag aagatgagct
    10861 catggggact ttcgggttat gtgagcctat ctggatgggg tacgtagaat cctggaggaa
    10921 gtggaaatcc cacacggatt tcgtacccta taagatagaa gaacgttatt tcaacgacga
    10981 gctcatgatc acgggaaaga tcgacttgat tgccgacatc gacgggatca agtacctttt
    11041 cgattggaaa acagcctcca cacgtagctc tcagtgggaa atccaacaag gcttgtatgc
    11101 tcttttgttg agagaagccc aaatcgagct ctcaccctat ctaagggtca ttcagctcag
    11161 caaagacggg acgttgccca aagtgcatac ctttgagatg aacgattccc tagaaaaaag
    11221 atgtcttcgt gaagtaaaga ttgcatggga caaagtacgc aaaagagaag aaatctagaa
    11281 gaatttaatt taatcgcaaa ccactgataa ttagttgcca ttaaatcaga atagtaatac
    11341 aattagcggc gtaacaaaaa cgcccgcgtg cagatagtag ctgccgcggg acctacaaac
    11401 ccacaacgca tggaaggcta tgggatttgc aacgcgcaca actatacgtg aagacgtatc
    11461 aaacgtcaac gacttcttcg ctgactcgtc atcttcacta gaaaaaacat ctagacaaga
    11521 gtatctggaa atcctaaaag ggctccttga gtcgtcgctc aacagctgca ccttaaacat
    11581 ccaggaggct cagaatgtct aataagattt cccctatttc ttcttacgtc ccttacggcc
    11641 tagaagatca gttttcccca gatctcttgg aattcctaag aggccttgag tccatcactt
    11701 ccagcatgca cacctttgcc tctacaacag agatcaaaga ccaaggcatc gcccaggcat
    11761 ctgtagatat gtgggccgat gcaagaacca tccaagagac tttggaagtc gcccgtaaaa
    11821 aggcccttca aaagtacgaa gcggaagtca aaaagatcaa cgctttagcg gaactgctgg
    11881 agaagccttt agacgagatt caacaaatct tctctcggaa gctgagtgat tttgttgtcg
    11941 aattcgagga agttaacaat cttttgggaa ccgatgcgga caagataaaa ggcaccaaag
    12001 gagccctata tcaaaaaacc acagaaagct atgaagtgga ggacatcgaa aaagttcccg
    12061 acgagtattt catgctggat gagaaaaaac tcaagaaagt tgtcggcgca ggcgtcgtca
    12121 tcccaggaat aaaacccaaa aaagtaaaat ccctaattat gaggcgcata taatggcatt
    12181 acccaacacc cactcattac aaaccattga cgatctgaaa aagaacctag aggttatgga
    12241 tagactatcg gggatcgcgt tatccacccg tctcttcccg aactatacaa aagagcagtt
    12301 gaccttgcta ttgctgacgg ctgcggaatt ggggatttct cccatgagag ccttgaatgg
    12361 tggcctcgta atagtaaaag gaaaagtatc catgtcggcc acgatgcaat gcgagcgcat
    12421 ccgacaggca ggccacagca taaagacaga gaagtgtacg gatcacgagt gcattttggt
    12481 cgggaagcga aaagatacgg gcgatgagat gcgtgttaat ttcactatcc aacaagccca
    12541 ccgcgctggg ttaaccaaaa atcctacatg gttagcttat ccccaaatca tgctccagca
    12601 ccgttgtgtt tctcttctgg ctcggtttct ttttgccgat atcttcgggt ctatttattg
    12661 catggatgag ggccgcgaga tcgcagaagc agaaggtgtg gaatttccca tcgcaatcga
    12721 tgatcagatc gcagaaattg cagaagaagc gcaagtgcta gagagcaatg tatctcaaga
    12781 tgtacaagac tctggagact ttttgcacga catccaagag ccccaacaag agcctcaacc
    12841 agctccagag accctactag gagagtttgc cgatgagcta catggttccc atttttctta
    12901 caccgacaaa gaaaaagttc tggattatct aagacatctc tttgccatca agaaaatgga
    12961 aatctctctt ttcatcgagg ctttacgggc atccatctca actccagctg gtcaagaatc
    13021 actaaaaaga ttgtttgaca actgggcaga aaaagcctaa agcccaaagc ctaaggtctc
    13081 agcttggccg cctttctatt gggagcactc tcaaggaaaa gcggctgagt ttgtttctgc
    13141 ttccaccgac ttagctcttc ttcgtatttt aacttgctct tgatctcaga ggccgtattt
    13201 attttttctt cgatctcatc ggggactata tgagcctctc catcttcaag agccctggat
    13261 ttcaaaaatt tctttgtcca aggggtcttg aaggcgactg tacagtagtc gtcaataccg
    13321 tacagcccca acaaataatc catgaaagcg atctcgggat ctgtaggaac atgacgcttt
    13381 agacacaatc ccaacatgtc caggaactca tcatgagtta tacgttttgt gatcttataa
    13441 ttttccaacg ctctgatctt aagcttttga tgacgcgtcg taacagacat cgttttgaat
    13501 tgggcttgcg tgcactgctt gacgtgctca tccgtatctg aggtccgtga tgaaaaaaac
    13561 tttttttctt catccaaaat agagaacaaa tcacgatctg gcagaattaa ttccgctctt
    13621 gtatcgagtt gttttttttt ggcggtcttt ttctttgagg acttttcttt tgtgaagacc
    13681 tggggaatac aggctatctc ttgatcattt gtccattcat cttgattgct tgatccggga
    13741 atatcctcga aatcccaacg acggccactg ataaaaggag catcgtataa aagatgacag
    13801 atactatcga gattgcgctg ggcgtgattg agaatacgat ggtggccact gaaaaacatg
    13861 tctctcaccc tcgtagaatt tgtagaattt tctacgtggg aagttacggc agaagaagac
    13921 gtcgaattat acataagatt caacagctga gcaaattttg agtcatttgc ccagcaatct
    13981 tatctcaaga aacattccga cgcaacactt ttcgtcgtga ccatccgttt agaccgctct
    14041 cggcacctcg atgccgtaaa aagaggccat ataggccaga tctcttttga cttgctcaag
    14101 agcgctcaac ttcagaggct gctcatcaaa gagcaaatcc actgctgact gcacacactg
    14161 ctggcactct tctctcttgc ccaagatctc tttttcgagg tcttctctta gaatgcccag
    14221 ttccgactcg tatccgtcat aaccccactc agaaggggcc tcgtggccat atccgtatct
    14281 catctctcaa ctccatgctg ttgattatta atgacttaaa gatatgtcct aaatataact
    14341 gacttagcaa taaaagtcaa atcaaaagaa aaaaaaaaga ggcattgctt tttacggcaa
    14401 cgccccttaa aaacaaggga ctaattttta acgtcccata ctactgaaaa ccgattgaaa
    14461 attagtcctc aacaccttac tctagaaagg agagtaaaat gcacctcagc tcacaagtaa
    14521 gaaatgttaa ccgagttaac aaaactcacc gaaaaccgag acactactat aaaatcatgt
    14581 atctggattt tatgcaagac ccttccatct cttttgagat aaaagggctc cttctccatc
    14641 ttttttcgct accagaaaca tggcaaatcc gactggatca gatctgcgcg cactatcgca
    14701 acgtgatggg ccaagacaag attacacgtt tggtagccca agcagcggag actcgttacc
    14761 tcagccggga aaagagacgg cgaccggacg gtcggtttga gcaagtatac actcgctatc
    14821 cgatgggaga tcgtcccggg ttcgaggaca aggtcccaga caaaaaggtg gaaatcctct
    14881 ataccgacga cgagcaagag aacggacggt atgaggagcc caatcccaaa gtcttaagct
    14941 tgccccaagt ccggccagta gtccaacgcc cagcccaaat cccagcccca atccaggctt
    15001 cccagccacc ccccaaatcc acccccccag atccccccaa atccaaggct tccgacccgt
    15061 cttctccctc tgatcagacc ccttcaccac ccccccattt tcacagggtg gtggaactaa
    15121 ggtcggccac atatataaag aaactagata aagataaaga taatcaatcc atcccaaacc
    15181 cttcctttcg gccgcttcgc gacctccagt cgggcccggg ccccaggatt gatcagattg
    15241 atagattttt tttgaattcg aaggaagaag atagggctta tcacgaaact ttagccaata
    15301 tcgagatgcc gtccaacatc ttgttgagtc tccgagagag agtagccatc tttgagcgat
    15361 gcaaccgctc gttggagatg ttcggcgagc tgtgctccag gtacagcagc tggaaagaga
    15421 gagctcggga gcgccccaag aacgacttcg gggtgatgta tgcttggaaa ttagacaaca
    15481 tggaacgaga gatgagcgag aagaagctag acgaccagcg atttgcagaa aacatcgagt
    15541 tcgccaagag ggcctacagg ctttgcccca agagtggctc agggtggaca gtcgacatga
    15601 gcttggccgt tgaggagccg tacttcgagc tcaggagtgg gtcttattca aaaactttct
    15661 tgataaaaga acccagattc gtcgacgagc taggggaaat gctggaaaga gctgggctgg
    15721 caaccaaaag tgaaccgcaa tactaccacc aggatgatcg aaaacatgct taaaaccgct
    15781 caggatcgac gatcgaccca tcagggctat ggagactcat ctgggtgtga gataagcgat
    15841 tctagatgcc tttctgttag tttttggaaa acgcctctat atccgcaaat gatcttgcta
    15901 tcaatccaag agataaccaa gcttggttgg actaccgaaa cggccgggtc agtggggttc
    15961 ggcaaatatt aagaaattgt taagaagagg ctcgagatgt ccaaaaaacc ttccgtagaa
    16021 gaacaattca ccgttgacga agacaaaaga gctaaactag ttgcgcactt attagatcaa
    16081 ttagaagaag aagcaccacc agaagataac aaaaaacgat ctaatttcaa aatctcggta
    16141 gccgaactct ttttaacaat ggttattcat gaagaagtag atccacaatc ttatatcaaa
    16201 ctaatagata gaataataga aagagttcac aaacacttag aatcattaaa taaaacgtaa
    16261 tgaaaaccga cactttaaat cacccgtttt tgttttttta tcttttttcg gcaattaact
    16321 tgcgttaaat cagcataggt attaaactga tggcatatac aacgcaaaac ccaaaaaagg
    16381 aaaaaaaatg accaaaagaa tggatctcga caaactcttc cccccccaga atctcgtcaa
    16441 cattttgaaa gacctgggca aagacccccg aataatccta gaggacgact acggatggtg
    16501 gactccacaa gagttcttgg cgcgatatcg tcccaaatcc aaaaatcgtc cgcgtggggc
    16561 gctcaacggg ggcgcgcaca tctataacgg gtcgctaatc atggataatg attggggagg
    16621 tcactgtttc atccccacgg ctcgactgcg ttgttgcgtt gtggacgata agggtcaaca
    16681 tgttctagat gaggatgatc aatacataga gctcgactac ggcacacgag actatattgt
    16741 agattgtttg gaggacctgt ggcctagata atattaacgg attatggtat acacacctcg
    16801 tgtcgtccct ctaacgaggg gcgtggattg aaactggttt tataaaaatt tagtgagtaa
    16861 actctcctat tttaggggac tagtcacaga cttgtccttc agactgccgc acacgcagtt
    16921 cccgcccctt cctagcagtg ggagggacgg tttttttctt gcaaatatct cgcgattggt
    16981 ccataatttt tgattaatgt gcgcgttaaa acaaatccgt cggaaatatt taattaaatt
    17041 gttagttttc tgggctattt gtaattaaaa attactaacc gagggaaata aagatgattg
    17101 acgacctaga caaaaaagcg gatactaata acccaacaac cgatccctat ctctttcaac
    17161 aagtcagaga tcttatctat cagagacaag acaactgtat tcaggacata ctactacata
    17221 ccgtcagtga gtgtccggga gagaaagatg actgaagaag aaatgagcta cgaaaagaca
    17281 gagcgtgtag aaaagttatt ccgtgacacg gcacaggaat tatctaacat tataagctat
    17341 tatctgaaga aaacaggaac gacgcatgag gggaccgctc tgctattcca attacaggtt
    17401 tttcggtgcc tggtggccga cactatctat cgtattgttc ccccccactt gtgtgatcaa
    17461 gtagtagatg ggctggccga tgatattaaa aaagagatta agttcctagt tgaaaacaag
    17521 ggcaccaaag atgactgaag gcgaaagttc ccacccaatt actatagagc ctactgtctt
    17581 aacttattgc caaaacgtct tgcattctat agccggatgg cccgaattcg tggatagcaa
    17641 atatgaatgc gatcgacaca tctttctttc taacctgggc attcctgaca tgcagtggga
    17701 tgtggataac cagtctttca acgaagcatt aaagattatg ggccacgcac tgaaaggcat
    17761 ctatgacact tatgagaaaa gatatgattt ctccgagcct gaattaacat tctgttcgat
    17821 gtttagctat tttacgatta aagttggtat gtataaaaaa aataaacaga gggttctcga
    17881 aatagcgggt cttgtgaaac tctgtgaccc agaagaagat gagtgactat cctagcaaaa
    17941 agtacaaaac cgtacatgtc tcaaaaaaag actttattga tcagcttagg gcagtatttc
    18001 tgactctgcc tagtgaacat atagcaagcc acctggcaga gaccctccta attattcaga
    18061 agatgactgt taaggacctc ctgcggcccg aagtaaaaaa agaattggaa gaagatatag
    18121 aaagatactc agatttcgta acggagataa ttagggaatt agaaacaaca tatgactgaa
    18181 aaaaccagag atgcagatta catcgtcgag tactccttga aaacaggagg cagtagtgag
    18241 agaaaaggaa gcaatgttca aaattttgta ctgggaaaac gaatttagat tctttggagc
    18301 ttgtgaactc acaccgtgaa ttttccatcc ataaaacaaa gaggcataag agaggttgtt
    18361 tttaaaagat cctttagaag acttattgaa agaaaaaata aaatggattc ttctgcaaaa
    18421 aaagcgttgt cgcagataac gagaatttta gaaaacagtg acctagactt tccccagaaa
    18481 aactacgtta tcaccgaact ctttgtgcag atggtaatgg agtgggggat tgatgattac
    18541 gcagtggatg acctctttga ttacatgtat gagggtgtgg tagatgcaag gttaaaattt
    18601 aagaaagcac aagctacgtc tagtataggg ctagaaaatg actgatccag catacttaca
    18661 ccgcagtatt ttcctcgacg tctccggcga tgagctggta gagtacattg ccgagggatt
    18721 cttggacaaa ggtgccgaaa acctggcact cagtcttgcc aagatcatgg ttgaaatcta
    18781 catgactgtc gataacgagc ttttgggcga cgctaagcta gagagtctgg acaagatact
    18841 ggcggaatac gcaaactttg tgaagaagtc cgctctagat ctgatcgctc gcaaggacgc
    18901 ctagcccgtt agccgcctag ttggccagtc tgagcctttt cagcttttcg ttgatgctcc
    18961 tgagctctgc ctttatctcc ccgagctcgc gaaataagtc ctccagggcg tttgagacca
    19021 ggtaactaga cctttctctt tcgtatggtg gggatgctgg cagattcttg atcggggact
    19081 ctggttcttg ggccaacaag gtcttttcat ggttttctct cattcgtcct aaaagagcgg
    19141 ttagatcttg gggggtctct gcatccagtc ttccgccgag ctcgtcgcac agaatccggt
    19201 tgaggtgggt cactaagaag cgtctaggga gaccgagccc acctttcaca ccactccctc
    19261 ttgtccatac tccgtggtcc agggcgttga gtagggacaa tagagcatta tggatctggg
    19321 ggggaatatc tcgatctttc agctcgcgga tgatccagga tttggcttgg tcgatggaga
    19381 aatcacgtgg atccagcaag ggataagtat caacggaggg tctatagttt tttgtaatcc
    19441 actcccctac ctccaatatt cttaataact tgttcttagg caatactcct atgatttctt
    19501 tcacagaagc gccggcgcac tttaggtcgt aaagcgctat tatttgtgga atacatataa
    19561 agaatttgag tctcttaaac ttccggaagt gagaacagta atattcgcgc tgttgaccca
    19621 tgcttttaaa aatagaatcc tcgcggccac tttttgcaga tcttagagct tctgcgatct
    19681 ccctgtaatg tttaaaatct aaaatctttc caaggcgctc ttcgtagatg ttagccatgc
    19741 agcacctcat aaatttttac cgaatctctg tacaggacca tttcggggac aatatttctg
    19801 atccgttttt ttacgtcctc ttctctaaac ccttcagatc ttagcctttt aaaaatcgaa
    19861 atcataaatc tgaggtcatt ctcggagaac cttgtattaa gatctcttag tttactgcgt
    19921 acagcctcta tgccgtttaa gatgtacgtc acttcagcca tttctggagg gttcactcgt
    19981 ccttcgcgga acaccaccct gtcttgtgag ttcacacagg cgatcgcgag gacagtcgtt
    20041 tttaacaaag ggtgatcgtc gactagttgc ttccattgta cgtaagcctc tgcgccgatc
    20101 tgcgagtaat acttgagtgt gtcccacagg gtccacgtct tggagttact gctcatcagg
    20161 cgtgcgcagt cgttagaggc attagagata atgcgataat agatagggat ttgcaaaatc
    20221 tcagcagctt ttagccggtg ctggccgtct atgacgcaga ggtctttgtc gacgatgatg
    20281 gggttgaaac ttagaagatt tcgctcgctg atcgagcgca caagtctcgt gacatgtttt
    20341 gcacagatgt cgcgattggt tggagaaagg ctgaatctcg catagtcctt ggtaacgtag
    20401 atctggactt cttcttggat gtcttccatt tttatttttc tcgtttatag gttctgtcga
    20461 tggcttgggt gttatcgact ttttcgtggt ttggctacaa ttatgcacac aatataaact
    20521 acttgtacac attctgtcta catctgtttc tcttcgcaaa agtgtcagtt atcaccactt
    20581 ccacaccccc agaagaagaa gaacataatt attttctata ttcttaatag agcggagtgg
    20641 attttgttga caattttctc ctggcggttg agaataaatt ttacttgtca attggaggct
    20701 cctatggcag aaccaaagaa agagaaaaaa cgccccggaa ggccccgtaa gcccgttccg
    20761 gctcattacc tggacccctc tattagacga ccaggaccag cgcccaaaga cgtcgataaa
    20821 gagcttttcg agtcgctgtg cggcatctgg tgtacgatca tggagatcga gagcattatg
    20881 aaaaccgatg cggcgacgat cgacaagtgg tgctttagga cttatggtgt tggcttctcc
    20941 agtgcttacg agcggctttg caacagcggc aaggcatccc taagacgcaa tcagcttaac
    21001 ctaagctcga ccaacgccgc tatggccatt tggcttggca agcagaaatt ggggcaatcc
    21061 gacaccccag cggaaagcgc gatgatcgag ggactcttgg caaaatatgt ggccactctc
    21121 aagcaaggcg cagaaaacac cttaaaacgc tctgagatcg acgattaatg cagccgatga
    21181 gcgtgaagca gtgtcacgcc cttattgagt ctgacgcgcg tttaaacgtg tttgaaggcc
    21241 ctgtgcgtgc gggcaagtcg tttgtctctt tggtgcggtg gatagatttc tgcttgaatg
    21301 ggcccccggg ggcgatgatt gtggtaggtc gaaccgataa gaccatcaag cgcaacatca
    21361 tcgtccccct ccaggagctc gcaggaaaga tcgtccaata cagcattggc aagggagagc
    21421 tcagggttgg tagtaggctc atctatgtcg taggcgccaa cgatgaccgc gcagaagcca
    21481 agatccgagg ttccgagttt gcgggagcgc tgatcgatga ggccacgctg atccctgaga
    21541 gctttttcaa gatgctattg tccaggctgt cgattcctgg cgccaagctg ttttgctcga
    21601 cgaaccccga ttcgccctat cactggctca agaccgattt tattgatcgg gccaaagagc
    21661 tggatatgaa ggtcttttcg ttcgatatcc atgacaaccc ctctctgact cctgagtaca
    21721 tcgagaacat cagcaaagag tacaaaggcc tttggtacaa gcgttacatc gagggcaagt
    21781 gggtgatggc agaaggtgcc gtctttgact ttttcgacga gcgtgtccac gttattgaac
    21841 atcctcctgg tgtgcctgac ggctatattg tgggcatcga ctacgggacg acgaacccct
    21901 gtgcctttgt gctcattggg tattcgggga gcacttaccc aaatatgtgg ctcgaaaagg
    21961 agtactacta cgactcgcgc aaggagctaa gacaaaagtc ggactacgaa tatgtcgacg
    22021 atctatgcgc ctttcttgac ggctacaatc caaaagccat ttacatcgat cctgccgccg
    22081 cgtctctcaa gcaagagatg ttgcgagctg gaataggtcg aatcgtagac gccaaaaacg
    22141 acgtactccc cggtatccgc tatcttggac agctcttgtc ggccggtaca tacaagatct
    22201 gcgccaattg ccacaactcc atcaaagagt acgccaacta tttgtgggac tccaaagcgt
    22261 ctctcaaggg caaagatgtc cccatcaagg catcggatca cgctattgac gcccaaagat
    22321 acgcgctttt tacgcacttc tttggcacca gcaccaaagg catgacggag gccgacgctc
    22381 ttgagatgga gcaaagatat ttctcttcaa tttaactttt tatttgtata aaagacgtca
    22441 actgctaaat tgatgcaatg gtaatattag gagattgtcc gtgaaatctt cgtctagtaa
    22501 atcgtgtaaa gccgaaaaat ctgaagtccg aaaacacgtg aaaagcgaca tcggggatta
    22561 caaagaggaa atccgaaaag acaagtctct cttaaagagt ctcaagcctt ccaaggccaa
    22621 acgcaaaaaa taacaccgtc tccccacagc ccaaaaccga atgtaaaccc gctttacaaa
    22681 caggtctgtc atggcaaaaa aatggattca aggcgctatc gaacatccag gagttcttca
    22741 caaggaactc ggtgtaaagc cagggaaaaa aatccccgag tctaagctga aaaaagccga
    22801 gcactctaaa aatccgacgc taaaaaaacg cgctgtcctt gcagaaactt tgaagtcttt
    22861 caagaagaaa tagggactac atgagcagca aaacaggagc tccagccgta aaaagaatac
    22921 ggcgccctta tcttaggcca gctcctgtaa agccagacga agacgatgcc aaaaaccatg
    22981 taaagcggcc ttacaagacc cgagaaccca tacccaagcc cattgtagtc cctaagcagc
    23041 cgatcctgtt gggaaaagag acgtggtata gatgctgtgg cggtcctcct ccgccctttg
    23101 agctagtcac cttgaaaaag tctctcttag acaggggaat gattgggtgg tgggacggcg
    23161 taaaatggga tggcctgcac atacgaccta ccgacgagtt tgaaatctgg aaaccgcacc
    23221 gcgctcttta atcttgtctg tgggtggata tggacggtat ttctatcgtc aacgagttca
    23281 atgatttcta tgaagaggcg tactacgctt ggaacccgta ttacccgttg gcggatgcgg
    23341 acatacgcgc ttatcttggc gatcagtgga cggagcaaga aaagcaaaag cttttccaag
    23401 aggggcgctc caactggtcg ttcaatctaa ttaggaaaaa catcaacctg gtggatggtt
    23461 accaaagatc ccacaggctt tcctccatcg tgttgcccca gcatcccaaa gaccaagaag
    23521 gcgccgatga gctgtccgac ctagtccaga aggttttttc ctcgggagat gggtacaaac
    23581 atctctctga cgccttttct ggtggactaa agaccgcatg gaacttggca accgtttgga
    23641 tggactatcg agatgacccg gtggacgggg atatccgatt tggaagagag ccatattcag
    23701 gctttattac agatccctac ctgacgcagc tggatttttc ggactgctct tatgttctac
    23761 gtagaaagta catgtctcca gagagggccg cttccctgct tcctgagcaa gccgacgagg
    23821 tttttgctgt ccacacagaa ggatggtcaa gagacgacaa gttcacatgg cttccctacc
    23881 agcagcagcc caacggccag gacttcatcg cgtacaacga gttctacaag cagatgtggc
    23941 ggcaagtccc tgttttggtg gacacccaga cgggcggtgc gtgggacatc aagctggata
    24001 agacctctct tggagaagtc aagcgtttcg ttaagaataa tcccacctat gccctcatat
    24061 accgctctaa gcgctttata gagtgccatg tgatcttgaa caacgtgtac ttcaagacca
    24121 tggaaaacca attcggcctg gacgagtacc ccttcacacc cgttgctgcc gtgtttgagc
    24181 ctgaaagcga gttctggggt ctaaaactac agagtctcgt cagagtgtgc caaaatccgc
    24241 agcgcctttt gaaccagttc ctggtgcaaa tggatgatat cgtcaaaagt caggtcaaca
    24301 gcggatggat tgctgaagaa gactctgttg taaacccaca atcgctcttc cagacctcgc
    24361 aaggccgcgt catatggaag aaaaagggag caagtccggc agctctggag agattgcaag
    24421 ccgctctcat acccgaaagc tttttccaga tggtcgatat cttctccaaa gggatttcgg
    24481 agattatggg cgtcaacgat gcagcttttg gcatacccga gagcggcaat gagtctggcg
    24541 tcatgatgat gttacgtcaa ggggcggcga tatccaacct acagagcgtt ttcgacaacc
    24601 ttaggtatgc tcagaagcaa ctcacacgca aggtcatcaa gctgatacag acgtggagcc
    24661 ctggaaaagt agagcgcatg ctgggaagaa agcctacaga gcagttttac tccaaagagt
    24721 tcgtaaagta tgacatctcc atccaagaag gcatgatgac ggacacgcaa cgtcagatgc
    24781 acttccggca gctcgtggat ctcaaacagc tgggagcacc cgtcactggg gcaatgctgg
    24841 caaaagccgc ccccatacaa ggcgcctcat cgtatctcaa agagctcgaa cagtccgaac
    24901 aacaacaagc ccagcaggcc caacaggtac aacaagtcca ggcccaactg ctcgaaggcc
    24961 aaatgcagtt acagaaggcc tctgcggtag aaaagatggc tggcgccaaa gaacgcttta
    25021 cgagatccgt cgccaacgtt ggactttctg acgaaagagc cgctcgcgct gtcgatgacc
    25081 gcgcctccgc tgcactggac agagctaaag cgatcaaaga gctcgaggcg atcgatgatg
    25141 aaagaatcat caaatacttg gatattgtcc atatgatgga gaaattaaat cagagtaaag
    25201 agaaggaaat aaagagcgac gatcttgcta tagccgctga aggtcagacc ttgggagaaa
    25261 tacaggcctc aacaatgtcg agagagctca ccggtaagga gcctgtatga atccttctat
    25321 gacagctagc acaaagaatc ctaagattgc ttacctcgtt catctgatgg gaaggatcaa
    25381 tgtggtgaac atgaagtttt ttgtcggttt ctttacaaac aaagcactca tggggataaa
    25441 agttaaaagc tctctgtcta taaaccctag aaccttttct aaagtttgga ttccttttgt
    25501 cactaccctt gaacctatga aaagcagatt gtttgcattc ataagaacag tacttagatt
    25561 tacgagatgt tttagagact ttgtgagaac cacactcaca acacgtaatc ttttctttcc
    25621 cttcgctgtg agtgcaccat ttcaaaaaac acttcctaga gcaaaaacta atgcgcttgt
    25681 ttttacatct agatacatcc gacggtcttt taacaaatat aatttggcac tctttacatc
    25741 ttatcgtgat ttttttattc ctagagagat cgtagcaaga ccgagaacag tatttggctc
    25801 catatctgga gaaggaattt tgacatgtgg ggcagatttt catcggccaa ttgtcgacat
    25861 tagccatgga taatgtcaac aatcaaccac cacacgaggt gaacaaggcg caagaagagc
    25921 agaccaaaga agatgacgtt gatatctcgg cgacatccaa aagcaagacc caacagctag
    25981 aagaaatgat gggtcaacaa gctttgggcc cgatggccca agggaaccaa aatataaacc
    26041 caacccaaga ggtggaaaat ggcacgccaa agccgttctg accgtatgga cgagtcccta
    26101 gggatgcgcc gtgggccaga aagagagatg aaagagtctt atgcttcccg ccgtcacgaa
    26161 agtgaaggta tgcgtcatga gatgaaagaa gatcgtcgta tggaacgccg agaggagtcc
    26221 cgcgaacgtt ctggcatggg ccgcgatttc aatcttaaag acaacgaaca agcttggatg
    26281 gtcaaaccca ttcctactga ttcggaaaat tacgatctaa gtcgcattaa gccctacaac
    26341 gatggctcta agggatatcc cgcagaagct tggaactacc gctactagat atcacatcta
    26401 agcacccatc acctttcccc gcctcctcac taccgactct caaagcaact caaagcaagt
    26461 taaccaataa cgagagtgaa ccatggctca agagacggga gagacccgcg acgcaatcat
    26521 tgaagagtcc aacaaacgcc tccaggacat catctatgcc aataggcaca gacaagaaaa
    26581 atactgggtc gttttgtacg cgaagccgac aagaaatacg gtcaacggat ttccagctct
    26641 tgcgcaacac ataaaagctt acggagaaaa gcccaagtca caagttggca tggtaattgg
    26701 agaagtagac cccttgaaat ccacgatctc ttgggaagtc aacatgcctc aagcaccttt
    26761 cgactttgac gcacttaggg ctcttggagc taaagcaggg gataccgtcg tcactgaaac
    26821 gacaaccatc cccggagctt atgtaacgca gtagcgccgc cagcttatgg gcgaagggaa
    26881 attatggaag acatacaaac gggcgtaaca caagaggccg ccgctcttac ggacagccaa
    26941 cctttagaac ccgcagaaca ggtgcatcaa gttccgcttg ctgcccttga gtcagagagg
    27001 tctaaacgcc agcagtacga agaagaaaac cggctgatga gagaacactt agcgctttta
    27061 cagagacagt ctcaagtcca acaagctcct agagacgatt tcggcggtct tcaagattcc
    27121 gatgtcatga cggttggtga cttcaagaag atggccacag gtatggcccg tcagatggaa
    27181 agctctatag aagagctcaa gatgcagcaa aagaaccccg actactcctc ggtcgttact
    27241 aagtatctgc caggagtcct gaaagaaaac cccagaattg ctgagcacct taaacagact
    27301 caagactacg agcttgcata ttatttggcg aagaacagtg acgaatataa atccgagcac
    27361 aagagggcgc agagaagcgc tgaggcggaa cgcatcgtaa agaatgcaga acactcttcg
    27421 acgctttcgg gggttggaaa taccgttcct gtagctggca caaagacata caagagcatg
    27481 tcggattctg aatttaggga actcatgtca agaaatctgg gctattaaga gtagcttata
    27541 gacgaggttt ttatgagcaa tatgaccact gtagccgtgc tgcccccagc agtgcgtgac
    27601 tattacgacc gcctcttgtt gatgaccgcc tacccaaccc ttatttacac aaagtttgcc
    27661 cagaaacgtg ttctgcccga gaaaatgggg gatcgcatcg tcttcagacg ttatgccaga
    27721 ctgcctacag tgcccattcc tttggtcgat ggcatcacgc ctcccggatc tcctctatcc
    27781 gttacggata ttaaggccca ggtggcgttc tacggtaact ttgtgacgat caccaatcag
    27841 gttcaactta ctgttgaaga cagggttaac atacaggccc tgtataaact cgatctaatt
    27901 gacttggagc tccttaatgc agcttaagga taacaagggc gaaggccgta aggccacgct
    27961 gaacgactta acgattgagc cccacaaggg gatgcgaaag tctgaactgc gaatatacat
    28021 gaaatcgcag agggagatcc gaagaggttt ccccgcctgg aaacaggtca caaaagtaac
    28081 agattgctta aacgaaagtt cgcgtttact cgcgcagaac ctcgcacaaa cgatggacca
    28141 ggtcaccaga gactgtttag cctcaacaag ttccgtacag ctatgccaga atgggatcaa
    28201 cgggaacacg cctaccgagc tgacaaaaca agatatcgac agcgcagtga aaaccctctt
    28261 gggcaatgat gcggagatga tttctgaagt ggtcataggc accaatcaga tatcgacaac
    28321 tccaattcgt ccggccttct ggggctacat tgataccgat ttgcttagcg acttggaagc
    28381 agtatccaac ttcgtatcgt cctcacaata tcctggcaac cagaatatcg tgttggattc
    28441 cgagtggggt gctacgggca acgtccgctg gctttacacc tccgtaggaa gcgtaagttc
    28501 agcagctacg ccagtttata acaactttat cgtcggtaga caatttgctg acgttaaatc
    28561 ttctctgatt gacttggaac tccttgaggc agcttgagga caacaagggc gaaggccgtg
    28621 aggccgcgct gaacgactaa gtgagaggac ttatgaaaat aagaagcgat agtctgaact
    28681 ctgcggtaac gcagagagga agatccgaag aggttttccc gcctaggaaa ctaggtcaca
    28741 aaagtaacag attgaaagaa gcctatgccg tggtgaacct gagaagcgaa acgggagatt
    28801 tctacgtcaa gcctcttgga tctgctggag cagctgaccc gcttaaccag cgtggctccg
    28861 ttggctggca gcacccattt acagcaagga ttttgaacga tgcgttcatg ctcaacttga
    28921 tggcaaccgc gtcataagga gaaaattatg gcccaaattt atacaacttc ctggactaac
    28981 cccgcgacag ccgttgccaa aaacgtctcc gtgggttttg aagtcgtgcg cgtacagacc
    29041 attgacgtta ccaacggtgg gtcgtggatg tgggtgtacg ggatgcccaa cggctattac
    29101 atcaacgtag ccacgggagc cgttacaacc tcgaatggtt ggacacctct tgccgaaaac
    29161 acacttttcg ctgcccccat caccgctgtg acgaagggcg ccgatacggt gtttacgtgt
    29221 tcgtatcttg atcaattcag ctttgctgta ggagacaccg tcttcgctac tgccatggct
    29281 gatgatctta cagggacgac tcttaacggt acatacaccg tgaagacagt ctcggcaaca
    29341 cagatcacct gtgaagagag cacagcatct ggatatagcg tgtacgtgag tggaggcttc
    29401 ctctctcaag tcagcgatgc agatggcaaa ccatatcctc aggctaacgt agccatcgag
    29461 ggcggtactg tcggtactgg aatggtaggc gctaactccg ctgctatggt cgcaatcttt
    29521 gaaggtagca acccagtagt ttaacgaatg taaaaccaga acctgtggtg ggggtcttct
    29581 tggttcgccc ccaccccttt aacaagtggt atgacgatga caaaagttct caatgagtct
    29641 atgtctaacg aagcactgaa tgccctaccc atcgtaccta ttgacggccg ccagcccgct
    29701 aacgaaaaag aagaaaaata cctaagagag ataggcgtct atgagttcca aaatctagaa
    29761 gatccagggc tccagatcac cttcccttac ggtggtacca agaaatcaca aaagtttata
    29821 ttaatgcacg gtgggcgcta taagttgcct agacacgtgg caaattggat agaaagccgt
    29881 ggtgagccta tctacaagtg gaagcccaac ggcgaaggcc gtatggaaag cacttattcg
    29941 ggtaggaagc ctcgcttcag cttaagacct gtgtttggga gctaattatg gcagcgacgt
    30001 ggacattgag ccagatacaa acgaaggtgc gtcagctcat agggagattt tcttccgctg
    30061 agatctccaa tgctgagctg atcacacgca tcaaccagta ttacacgctt accttcccag
    30121 cagaagttaa gcttgagacg aagcacgttt actatcagtt caccacgtcc gccaaccagc
    30181 cctattatga catcccgcag actacctaca cgaactttga gcccccagct acatgcaaca
    30241 atttgcttat gtactggttc caagatccag cctatttctt ccaggcaaac cccctccagt
    30301 atacctttct aacgccttgg acgggtgacg gaactacgac agcgtttaac accacgatca
    30361 ccggcttccc gatatatccc gggacgacga cgatttatga cggtgtcgag ctgtttgaag
    30421 ataccaacca gacctatacg acgtccaacg ttacggtaac tggaagtctt ggcggatcga
    30481 tggtcttgaa ctattcgacg ggtgtcattt cggtgacttt caacacagct cccgccaacg
    30541 ggcagaatat caacctgaac tatgtggttt tcgccgcgca gaggcctcag gcgatcttgc
    30601 tgtataataa ccaattccag ctttggccgg tgccagacca agcctacatc atcaagatgc
    30661 gtgcttgggg cgttgtagat gccctggtaa atgctacgga cacccctcag ctcaacgaat
    30721 ggggccctac gatagctttt ggcacagcgc gccagatcgc tgctgactat ggggagatgg
    30781 acgcatatgc ccagctgacc gtactttata aggagcagct cggatacatc ttgacgcgca
    30841 cgatgcaaga gatgatgagc tccagagctc ctccaacttt ttgatgtaaa accgctttac
    30901 aaggtgcact atggcctggg atatcacaca gcccacagat aatacaaaga tccgagattt
    30961 gggagtcgtc atccgaccaa actggcaagc tattcaaacc gctgactcta gctttacacc
    31021 ggatgccatg aacttaacca acagaacagt agcggcttta cctgtagatc caacggctat
    31081 tgctgacgcc tacgtcatgt actgtaagac agacgctgcc ggtaattcag agctttttgg
    31141 catcgatcca aactctgtga tcagccaatt cacatccacc gattacacgc tggcacaaga
    31201 cggatacgcc gtcataccac ctgggatctt gatccaatgg ggtagagatc tgatggtggg
    31261 gggaacgtct acattggcag tgtcgtttgg gaaggctttt tcgtcgactg cgtgggtagt
    31321 ggtgtcaact ccctatgaca accccattac gggaagcact ccaagagagt ggggtgttaa
    31381 caatattaca gctgctggct ttacagctaa ttccttcaat ggagtagtcc caggtggagg
    31441 ggtaaatttt ggatggtttg caataggccc aatgtaggtg ctcgatgact caacctcaat
    31501 cttctggttt ggtgcctgtt ttgttggcgc ctttttctac gggcttggtg aacaaccgag
    31561 aaccgtggct tatgcccgcg gatgccttcc aggaagtgga caacttcaac atccaccatg
    31621 gatggctaga aaagcgccaa gggtaccggt tttggggaaa cttgggctct agcctgcgtg
    31681 tcatggggct cgtgtactat ttggagaatg atggatccaa gaccaacctg gctttcgaca
    31741 caaagtacgg atacatctac aacacgttga caaacgttta cgacgatctg gactcagggg
    31801 ctcagacact cgacggtgga gagtacgatt acatttggtc ggtcaactgg caaagttcca
    31861 acatagacaa caggctctat tatacgaatg gcctgccgta tgatggcaca gcctacaatg
    31921 ggattcgatt ttaccctaaa gacgccgcac accttaccga cacggatctt cttaaaccca
    31981 gtttaggggg aggattgaaa ctatatggtg ccaagttgct ctttaccatt gggcaacgac
    32041 ttgtttgcct ctatacctac gaaaataatg gagcgtctac tagttcatac ccacaaagag
    32101 cgcggtggtg tgcccgtcaa gacccaagca actgggatga tacagttgga ggcgggggtg
    32161 cgtatgccga tgcggcaaca ggcgaccaga ttatttctgc ccaagctcta caaaaccaga
    32221 ttatcgtttt ctttaccaat tctgtctgga ctctgacacc gacttccgat ccaaataaag
    32281 ccttcaaatg gtcacgcctt aacaacttcc gagcttgtga tggcaagatg gcctctgttg
    32341 cttatgatag atactcgaca gctgtgggta ttcgtggcat tacttccacg gatgcgacgg
    32401 agacccgtag gtctgacacg cagatccccg attttgccgt cgatgagatt aactttgcgg
    32461 agttcaaaaa ggtattttgc tttagggatt acgcatcttt aagatggtgg acactatatg
    32521 cgtcagaaga ttccacggag aacaattcgg cccttatata cgacgacgaa tcccaagcct
    32581 atactacgta ctccatcgcc ctcaactgtc tgggctttgg caatagttct cttgattaca
    32641 agctttctga ctttacttct gctaatggac tagacttctc gttgaacgat atgagcgagg
    32701 aaaccctaca gtctttttac ctagaagcgg caaccgacac attacttgga ggcactacta
    32761 atggcttcgt ctatcagctc gatatcagct cacaggatgc tggaacagac tttggagcct
    32821 ctttcacaac agcggcttgg aaccctttcc agattcaggg actcgaaagc cagatgtcct
    32881 atgtggactt ttacgtggat accgacccct tcacacttgg aaccgtggag ttctataaga
    32941 acgaccaggt ctccccctat gcttcacaac cccttgtctt tttgcctaac ctggattacc
    33001 tcacctccat tgcccaaata gataagacca gtccttgtgc tgtaaatgcc cctcagcatg
    33061 ggcttacaac gggggatgtc gtcttcatct acggtgttca agggatggta caagtctccg
    33121 gcgaatacac agtgaccgta gtcgacgaaa acaacattac tttagatggc atcaacgcaa
    33181 cgggatatac cacctatgtg ggaggaggag cgctctatag aagagagttc taccagacga
    33241 agacgtggat aagagcttat ggtggtggta taggatatct gcattggata aaggtaacca
    33301 tcaatgaagg gaaaacgccc atgcgtttcc atgccatcaa gccgtacttc cgaccgatcg
    33361 gcactcgaac gattaactaa tcgaattaag gaatggtcac aatgcctttc aaatctaaag
    33421 cacaacgccg atggatgtat gccaacgacc ccaagatggc ggcagaatgg caagaagaaa
    33481 cgcccaaagg gaaaaagctc ccagaaaaag taaaaaagaa gaagcctaaa aaatgagcct
    33541 acccacagat tccaatctgc ctctgcccta tgactatgac atagccaaag agcccgataa
    33601 atatttaaac gacctcgtat ttcgcctcca ggagatgtac gaagagcttt cagaagtagc
    33661 tggagggtcg ttccgttctt atgctacggt agatggatct cagtgggtgc ccacattgaa
    33721 tggtacaacg tcaagcggct ttacatacac caaccagtat gggtggtcat gtcggtcggg
    33781 aatcatgaca gaggtttggg cggatgtgga atggagtgcc actacggcta ctggagacat
    33841 ctattttgag ctgccttatg aggtcacgct tagctcagga atgccctttg ttggggtttg
    33901 ccaagcgtcg ggcatagctg ctaccacggg tacaggtttc gtcatcaatg ccattccagg
    33961 cacttaccgt ggcgagatat ggaacgttgg aagcggagta gcaacgggta atcaagccgt
    34021 tgttgcatct ggtcgtctca tagtctactg tagatacata ggaatttcag acggttaacc
    34081 acaatgaaaa aaatagatga actaagatgg gtgcgtgcct tttcccctga cgttatcccc
    34141 aaatacctta tcgagcagat caaaaagcgc gactttgaag tagacgactt cttgcgatat
    34201 cacaaggtcg catgtatgcg ggaaacgcaa gacggattta cattgaatcc cctctcgcaa
    34261 ttatgggttt tagccaaccc tgataacttg gtgaaaggcg tcctttggtt tgtcgtcgac
    34321 cccttatgca aggccatcgt catccagaca tactccgtag atcccgagta ttggaaggac
    34381 ggcggagcaa tggaaaaatg tgtgtcccat gttaaagata tttatagaaa aactgggatg
    34441 aaaaaaatat actggataac agattacccc aagcataaca agcgtcacgg cttccatccc
    34501 tctaagagca ttttgatgga atatcgagaa gaggcgccag aagatctaaa agatccagag
    34561 gagattgaag atgggcaaaa cacttatggg gggcgcgagc cacagcggga acgcacaact
    34621 cctgacaccc cagcaacaac agagctttaa ttccctattg tcggccttag gtggacttgg
    34681 ccagcaagct tattcggact ttttgcagcc gtatagccct cagcaatacc aagatttcta
    34741 taggcaaagt tttattgagc ccgcgcagca agccctcaag caagacatca ttccagcgct
    34801 aaaagagagt atgttgggcc ttgatgagag tggatcttct gatctcaata gggcactttc
    34861 ccgtgcttct gttgatcttt cgtcgctctt gggacaagga atactcaacc agtacaacca
    34921 gagcaatgct aacagactgt cggcgctttc ggggtatagt gggcttctgg gagccaagac
    34981 ttttgagccg gtgatctcac agagttcggg gattcttggg ccattgattg gcttgggtgg
    35041 tcagctgggt gccccttctt tgatgaggag atagttcatg gcaatagtcg tcaacaaaga
    35101 tgccggactg attcaaggat tacaaactgc tggcaatgcg ttggcggcca ctctgaccca
    35161 taatgcgctt atgcgggctc aggaaaacca gcagaaaaaa caacaagctc aatacgctca
    35221 actggcctct aaactccagg gcaagcccat cgaccagaat cttctgttct cgttgttggc
    35281 cgagcctggt ggcatggaag ctctcaaaac cctagggcct cttttgggag gagttctcaa
    35341 agaaaatgct agatccgctg gcgcaggaaa ctggttgcaa aatgctgttt tgcaagccac
    35401 tggtggtcat ccccaaaatc cccaaaatcc agaagcgcct cagatgccat atggcattaa
    35461 tcagggcgtc caaagtgctg gacaaggcat cctagactct gtgatgggat cgcttacccc
    35521 catagatcag cccatgaatg atggatacca tctaaacaag gatttctacg agcgcaatgg
    35581 attggcaaag cccgatatca gcgacctaca ggaatggcgc agtggcacct tgaatgctcc
    35641 cgctgtctcg ggagtgccct cagcacctcc tttgcccgat gaaaatcgcg tcggccccag
    35701 ggaacagaca aaattccaga tgggcggaca gagtggcatg gatctacatc cacctcaaga
    35761 gaatttccgc caagttccca tcaagcagcc catcccacag atctcggatt taaacgcgga
    35821 tcaacaggag aaattggccc aaatattgtc cggaagccct tatcaagaac atcgggatct
    35881 agccaaaaat attcaagatc aagtcaaaca gaacacaagg aatttccacg aagacaggaa
    35941 atacca
//
LOCUS       DAOQLC010000030        35715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_360_length_35715_cov_22.668929, whole genome shotgun sequence.
ACCESSION   DAOQLC010000030 DAOQLC010000000
VERSION     DAOQLC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 35715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_360_length_35715_cov_22.668929"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3877)
                     /locus_tag="PL000_07325"
     CDS             complement(<1..3877)
                     /locus_tag="PL000_07325"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HNC88745.1"
                     /translation="MFEKKEYFYKVALFIGMAVILFLVSGIFQVDAASAALGQITATP
                     TPVVTTLPPNGSNVQNSFGPSGVGPFVSTIYYVNISGGNDGNTCTTIAAPCQHIQEAV
                     NKAVAGDVINVATGTYTFSSNGTPNVVIINKNLKLSGGWNSGFTTQTGASVIDGANAN
                     NGILAVSGTVNVENFVVKNSKSYNGGAVYIVNGSFTLTRSTLMNNVATQHGAGIFLDN
                     GTLNVVNSTVTGNKATSGGGGIYVSNNAAGSAFIQNSTVAYNTAGTGGGIRRSNGTYN
                     ITNSIIANNSSSNSGPDCAGTIAVANYNIIEDVTGCSITGGSNNLNVDPAIENSLSGS
                     ILSHALMLGSPAIDAGTSVGCPSTDQLGLSRPQGSACDIGSIEYMDFPPTVLEVTRAG
                     LNNTSAALVAFAVNFSENVTGVDANDFALTTTGVSGAVISSVSGSGSIYSVTVNTGSG
                     NGTIRLDVLSNGGIVDAGANSLSSAFTSGEVYTIVKTGGGGPSLIAYSDLVVDEGRNR
                     LYGADKNGNKIDVVDLTDLSVIGSYTLAYGTSPISLDLSPDGNELAVAQSGTGYVAFV
                     NLSTSSISQIPSALSGSSTKVTDVIYGRTGILYALSSNGLHVIDLTLSPHSEIVTQYL
                     YDTEVYEQRFGAISSDKNTLYIVTGTCCSGYDSLGKYNISNGLAKPVLLDRTHLYATG
                     YVKNIRLNLINDENLLVSNFAVYNTSNLTPRARNSLYSLVTPAATLPGKDYYVVLYDN
                     GNLETDTLNFHDVDGSYPLSSASTNVVGTPGAIVANNNGNTLFVSSTGGMAKFNLGVT
                     PPGDAVPLPTSLHHYRDFVFDIPRGVAYGTDESGRVDVVSLSTSSIVKSYLLPSGANP
                     IGIDLSPDGSELAIALNGLEKLLFIDPVTGATIAEVTPDLSDSIYYVNRPFDVIYGRS
                     GRLYSDGDPVGGTDYLHVINTVTHSWVSKSPYPVLLRTGPELEISADNQYVYANEIFS
                     PNNIFVLDVRTDNITKLYSGPHGPVSAPKFTIAPDGSKIFTSSGQVWDGIMQSQLGAL
                     EGAPGNLIEFIPGQNMLVLSDIDTGSDVLKFISAVDYHLISTYTPSSPGGSITEMEVS
                     PDGNMLIMNINNEVRVMSLDPTAPAGAWTVGGDGQATTPGTQFPAPLVLLIENYLGQP
                     IVGRNVTFTAPASGPSGTFQGTNSRIATAVTDANGHAISPMFVANNQQGTYNIIASVN
                     GWEIPFELINVDFAPYVTSVYRISPDPTAAASVDYTVTFSEAVTGVDKDDFTLTTSGV
                     TGASIATVTGSGSSYTVKVNTGSGNGTIRL"
     gene            complement(4152..4919)
                     /locus_tag="PL000_07330"
     CDS             complement(4152..4919)
                     /locus_tag="PL000_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88746.1"
                     /translation="MPITHETQKTLINDKAETGDALCMQVRDLHFSYPDGHSALHGVS
                     LKLCNGDKVALVGPNGAGKSTLMLHLNGILSGNGEIEIRGQRLTRDNLPAIRAMVGLV
                     FQNPDDQLFSPTVFEDVAFGPLHMGLPEVEVRARVDAALEAVKMTAYKDRLSHHLSVG
                     EKKRIAIATVLSMNPSLLILDEPSAGLDPRARRTLINLLRELPITMLVSTHDMKLVQE
                     LFPRTIVMDEGRVVADGLTTEILENEELLTAHGLEKP"
     gene            complement(4919..5710)
                     /gene="cbiQ"
                     /locus_tag="PL000_07335"
     CDS             complement(4919..5710)
                     /gene="cbiQ"
                     /locus_tag="PL000_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HNC88747.1"
                     /translation="MHADAFDRYHDKESFVHRLDPRVKTIVTVVFIVSNALLPDGAWL
                     AFVLAWVFLLGINLLSNLGFMYTFKRSFVALPFALVAVTVLFSIPGNPVSSFHFLMWD
                     FTITDAGLLRFVSILIRSWLSVQMAIMLVAVTRFPDLIHALEHLHVPAILTTIIAFLY
                     RYLFVLVDEVYRLLRARESRSAAVSGQRSGGSVVWRAKVAGNMAGQLFLRSYERSDRI
                     YNAMLSRGYTGHLYTLNPHEMKSRDYLVTALVVVLIFVMQILGRL"
     gene            complement(5726..6667)
                     /locus_tag="PL000_07340"
     CDS             complement(5726..6667)
                     /locus_tag="PL000_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNC88748.1"
                     /translation="MFYSPAPLHIPDGFLSVLVSIICWVITVITLGIAISKTNKSLGE
                     RQVPLMGVMAAFIFAAQMINYPVLGGTSGHLLGGALAAITLGPWAGILVMTAVITVQG
                     LLFQDGGLLAMGANILNMGLLTAGVGYGLYRSVLGKGRGVQLAVAGVAAWLSVMAGAL
                     ATSLQLWLSGTSDLRLVIPAMLSVHALIGLGEALVTVAALGFIFRTRPDLLDEKSASA
                     QGGRGWVVAGVLITLAVLLLSPFASADPDGLERVAINMGFIESGQSAPYEIIPDYAIP
                     FLGETPISTIVAGGVGVLVVVAVAFLLGRSLQQRNAG"
     gene            complement(6672..7097)
                     /locus_tag="PL000_07345"
     CDS             complement(6672..7097)
                     /locus_tag="PL000_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNC88749.1"
                     /translation="MTCADLYTPELRARGYRMTPQRMAILHTLHHAGTHLSPTEIYEA
                     AKKDLPGLTEPTVYRTLEFLAENGLARPAHMGNGHLVYEIARHEHHHLVCRICGDEME
                     VEHALLKSLYQKLESTSGYKLTDSHVTFFGLCPSCQKGK"
     gene            complement(7367..8074)
                     /locus_tag="PL000_07350"
     CDS             complement(7367..8074)
                     /locus_tag="PL000_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88750.1"
                     /translation="MDFKKLFYLLVLITLTACTLPVDEAPPPTALTTATPQPALTLPA
                     TFTPDPTPTYSPTPTLAINEIRTPVQFYDFCADNRGYILIEELIQAITQENGTAFEKL
                     VSPAIGVDIRYYRNDKVVNYTLDTASIFQDTAEVDWGISFGSGQPTVGTFREIVLPSL
                     QKVFTPASLIVCGQLQIGNASYFPEWPYPDMNFYSIYFPGTDEFGGMDWQTWAVGMDT
                     ITGKPYLAALVHYVWEP"
     gene            complement(8145..8555)
                     /locus_tag="PL000_07355"
     CDS             complement(8145..8555)
                     /locus_tag="PL000_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88751.1"
                     /translation="MTQKNKKEGLSAGDYGIVIGGNVNNSNIVMGDGNTVSLSATSLD
                     SFFKDMEQMVDEQPNLKPDDKEDIKAELQEIQVALKAKEPDETFLARRFRNLKRMAPD
                     ILEVAIETLKNPIGGVAEVIKRVAKKMAEDAAAS"
     gene            complement(8602..11235)
                     /locus_tag="PL000_07360"
     CDS             complement(8602..11235)
                     /locus_tag="PL000_07360"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC88752.1"
                     /translation="MTEKKQPSKKTSKPSQPEQEIQAGDQSIAVGGSVTDSAIIHGET
                     IVLADRFWRDLKPALPPEKLREATAAYLAYLTDRHYYLSLKGMGVSDRVPLKLKLLDL
                     YVPLKARMELPEGETWKRDVSLAGRNVSAEEEEMFNLGEPVPLLKVMQKHSGVIILGD
                     PGAGKTTFVKYLALRLARGEGKKIGLNDYLPILLPLAAFANAITARDVRLDDFIAEYF
                     DGIGADLPVGPMLKEALKAGRALILLDGLDEVRDISLRNTVVERVVDFFAFHRREGNK
                     FVLTSRVVGYRAVRPSAEDLVECTIVDFEEDEIEEFVKYWTTALEKQAQGNTAVARAD
                     AETDRRELLDAIQQNPGVRQLASTPLLLTILALMKRQGVSLPERRVQLYDQYVNTLLS
                     TWNRARSLSGRAPGRDIDELQTVRILAPLALWMHEVSPGVGLVGREDMRRKLEELFLE
                     RGDVSPHQAARQFLIDVREHAALLLERGPGEYGFIHLTFEEYLAAVALALMGQGNPAP
                     IIEVLSAHVGEQAWREVTLLTIGYLGIRQQLPKVAGEVVEALVNNQPGPPGEAVVLAG
                     DAVLDTWPGGVSPQNREYVIQALVDTMQDGAIRPDLRRHASLLLGRLGWRPGDLDRFA
                     EIPEGEYQAGIKKEPKTIPYNYFIGKYPITNIQFARFVKEDGYHTQEYWSYDGWEWRT
                     GKYDSRTLEAVAHDWLEHRPLAKRNIPYYWHNIELSNPIVPVVGVCWFEAEAYCNWLA
                     KKIVAVPEGYTIRLPREGEWERASRGEDGREYPWGEGFDKAAANTWDSDTKGSGLGGT
                     TAVCTFPAGVSPNGVWDTSGNVWEWTSSWYDEDRKYRVVRGGSWIGYQWFARSSFCNW
                     SIPLMFNDDLGFRVVIAPKDL"
     misc_binding    11436..11751
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11796..11948
                     /locus_tag="PL000_07365"
     CDS             11796..11948
                     /locus_tag="PL000_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88753.1"
                     /translation="MNPVTEQDEQTTYMQERAIETPTDDSLPAAQVIEEQNKEAEQNY
                     TPRFIP"
     gene            11970..13337
                     /locus_tag="PL000_07370"
     CDS             11970..13337
                     /locus_tag="PL000_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNC88754.1"
                     /translation="MENTTRFLLNETDLPKFWYNIAADSPVAPNPVLHPGTLEPVTPD
                     FLSVLFPMDLIMQEISTERYIEIPEEVREVYKLYRPTPLLRARRLEKALDTPAHIYYK
                     YEGVSPSGSHKPNTAIAQAYYNKIAGTKALTTETGAGQWGSSLAFACSVFNMELEIYM
                     VKVSYHQKPYRRNLMEVYGATVYASPTDRTNYGRSVLAENPDNKGSLGIAISEAVEAA
                     ATSNGTKKYSLGSVLNHVLLHQTVIGEEALKQMDMANEYPDVVIGCAGGGSNFAGIAF
                     PFLRENLKNGKRARLVAVEPTATPSLTKGVFTFDYGDTAKMAPIVKMHTLGHDFMPPG
                     IHAGGLRYHGMAPSVSALVDAGHIDAVAVKQRETFEAAIQFANAEGIVPAPESAHAVR
                     AAINEAMDAKAKGEKRVILFNLSGHGNFDMAAYESYLGGKLEDFEYPAEAVQEAMKKL
                     PQVSL"
     gene            complement(13396..14556)
                     /locus_tag="PL000_07375"
     CDS             complement(13396..14556)
                     /locus_tag="PL000_07375"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNC88755.1"
                     /translation="MALFASVGSAQALDGREAGLQAAHQALNHLGANSANFAIVVASH
                     QYQAREVLNGVTSLLGELPMVGFSSPAGLTSEGHFQHSVVVALLSGDFQADAMWLPGY
                     AQSGRETAAKVADHAAAREERQSLLVFADGFNGDAEQFCGAMPPGLNILGALSSGDLN
                     TGNTYQMTGTQTGTGGMVAAFLRGEIKTGVGYGHGWDQVGKQFRVTRSRGFWLRTLDG
                     RPASEAYAALFGYPARDWAFPPLSYLARLYPLGVEVGDDLITRSPIRVEADGSFRMNA
                     SIRDGIDAYLLVGSRAACERAARDATQQALLQLGDSKPVFALVLVDVAWQMLLKAHPG
                     VEIEAVRDILGTNVPIAGGYTLGQVVSSENVTPKFLNQHIVVVVFGEPVHNS"
     gene            complement(14566..18627)
                     /locus_tag="PL000_07380"
     CDS             complement(14566..18627)
                     /locus_tag="PL000_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC88756.1"
                     /translation="MAARNFQRSAQINLPRNGTLFNQILQWTVFAYQAIAVIIFLVGM
                     YLSVRWFQQPFLGALYEHTMVFNGTGPSEAMPEWALFKQVVVGDQMVAIDGTPVRNTR
                     EVRSILQGHVPGENVTVTVKSESGQSRDVSVTLHNFPASSRTTYFIVPSILGLVFIIA
                     SWWIFGLRRNEPAGRAFSLFTSSLAIVTGAYFNLVTTHEFTVFWTFALAIMGGALFDL
                     GLSFPLEPRVVINRPYLRWVGLIIGIVLAVFNLTTLFNFERPTAYIPIWQYIYGFNAI
                     SIIFYIGLSLYQAVSAQSPVVKTQARAILLGAMLAFVPAGIWLGLNAFQLLWKETGSS
                     ASQPLNFSPYLFLPLAFFPLTIGYTILRFRFLRTDDFVRRGLMYFFLTAFVMGGYGLV
                     VAGVGLLVNSSSSDALVSNPYLVGGFMLLLALLLEPFRVRLQSVVDNTFFRGERALAE
                     KLEDFSHKLATALDLNSISTILREQIASTLTPSRVHIYTYDSLNDFYSALPGEDRRPT
                     SDIRFTTTSPFVRYFEKEHLPLYLDTTAALPSDLQSEQSRLALLGARLFVVLPGKNRI
                     NGWLALGPRLSGQPYNPRDLRFLENICDQASVAIERMQTVARLERQIQEMNALTRVSQ
                     GVNVTLTFDDVLELIYAQTAQIIPTSHFHITLYNEASDYYYYGFCVENNERISERENL
                     PFPVTAGLSPEVIAKSRQIMTQDYIHECQARSLTPIVENVFAWMGVPLNAGAKSIGAL
                     SIGSRDGATAYTRGQLELLQAIADQTAGAIVKARLLEETQQRAHQLSTLNEINRQLTS
                     TLELSPLLQNILENAVGILNCEAGSLFLVDEQTKELVFKVTVGPVAANLIGQRLPPGT
                     GIVGRAVQSRGPVIENEENRSASRFRGVDQQTGFVSRSLMAVPLQVKDRVIGVIEVIN
                     RRDGLPFVTGDQDLLTAFAGQAAVAIENARLYTLTDQELAARVEELSVMQRIDRELNA
                     SLEMDRAMHITLEWALRQSDAEAGLIGMLEEGKLRVMAHQGLDDRMANLPEDQAMKIE
                     LPAMIQSIENGQPQSLSVATSNDKLLSSAHTQMIIPIRRETTVIGLLHLESISDSQVD
                     IGFLTRLTDHAAIAISNAQLFGEVQRANLEKSRFVSFVAHELKNPMTSIKGYTDLMAA
                     GAVGPISDMQANFLNTIKTNVIRMDTVVSDLNDISKIEAGRLSLAYKVTEVPPLIDDT
                     VRSLKRQIEEKKQTLEIVLPEKLPVIWADPNRITQVLVNLLSNSYKYTPESGILQIGA
                     EEASNLGETNSSMRVVHIWVKDNGLGMTPEDQKKVFQQYFRTEYSKEVASGTGLGLNI
                     TKNLIEMQGGSIWFESEFHVGTTFHITIPVAEE"
     gene            complement(18795..20726)
                     /gene="gyrB"
                     /locus_tag="PL000_07385"
     CDS             complement(18795..20726)
                     /gene="gyrB"
                     /locus_tag="PL000_07385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNC88757.1"
                     /translation="MAEKEKKINYDVSSIQALEGIEHVRKRPGMYVGGTDIKALHHLV
                     YEVVDNSIDEALAGVCNRINITIHPDSSVTIEDNGRGIPVGPHPSKKDANGKPMETLD
                     VVMTVIGAGGKFGGGGYKVSGGLHGVGVSAVNALSEWMVTEIKRDGKLWRQEYKRGVP
                     QGSIKQIGKVKDETGTKQTFRFDKQIFTEDIDFRFDTLVQRFREMSFVARGITIKFVD
                     ERADREMTFYFEGGLTSFVRYLNRNRENLHPVVHVEKEIENIGIEAAIQYTDAYTESV
                     YSFANTINTVDGGTHLTGLRSSLTRVINDYARKNGLLKDADPNFSGDDTREGLTAIVS
                     VKHPDPQFESQTKVKLMNPEAQTYVTQVVGETFGTFLEENPQAAKAIIAKCLTSARAR
                     DAARKARDLVIRKSALESLTLPGKLADCSERDSSKTELYIVEGDSAGGSAKQGRDRHF
                     QAILPLRGKILNTERARLDKILGNNEVRSLISALGTGVGDNFDLEGLRYGRVIIMTDA
                     DVDGSHIRTLLLTFFFRYMPLLIDEGHLYIAQPPLYRVAYKNQVKYAYSDKEKDKLVK
                     EIGSDKVGIQRYKGLGEMNPEQLWETTMNPTNRTLLLVTVDDAAEADRTFDMLMGDAV
                     DPRKKFIQTHAKSVRNLDI"
     gene            complement(20771..21760)
                     /locus_tag="PL000_07390"
     CDS             complement(20771..21760)
                     /locus_tag="PL000_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88758.1"
                     /translation="MEVSRTENLPTPPGIINSIKAGFDMIASHITAILLPLLLNLFLW
                     LGPRLRMDALFNSIKDDVVSIWRTGGVPAEDIQRILTWYETTIPNINLFWIVRTLPIG
                     ISSLLFPQQPAATPLGNPLVLQVDAVNLMGWVFLLNLLGWVGGGLYFRSVAWLTVADK
                     ENQPIQISRAIVQTVLTSIIWSILSVIILVPAFLVLAVVLQFSAFFANLLILFMSLIS
                     MWVIVPFFFWPHGIFLRRQNFLTSILSSVQMTRFTLPTSSMFVLTVFLLSVGLNFLWS
                     IPPEDSWMTLLGIFGHSFVTTALLASSFVYYRDMTAWLQAVIEKLRPNAVTKA"
     gene            21823..22638
                     /locus_tag="PL000_07395"
     CDS             21823..22638
                     /locus_tag="PL000_07395"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88759.1"
                     /translation="MQERHSYLPSADRVGVLIASVLLTFALTRLIQSPQFTLTIELPG
                     FYFAYPLTLGTAMTILAAALAAAGMDWLTRGHPNLGEKPNIEHLMLPTLTTFVIGSPL
                     ALLPESIAWWVALAFAASLLVIVFLAEYITIDPSAPSYAFARAGLTALAYALFLILAM
                     SLRFSGVRMFLLFPVVLVVAGLISLRILHLDGTDRWDFPWALGIGIVCAQIGAGLHYW
                     PLTPIQFGLALTGPLYALTMLSASLTENTPLRRAAVGPILILGLSWVSAFLVK"
     gene            22655..23053
                     /locus_tag="PL000_07400"
     CDS             22655..23053
                     /locus_tag="PL000_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNC88760.1"
                     /translation="MNSLTLTNEQLQKMIDHVDSHAPLEACGLLAGKGSTVEAVLVVR
                     NQAQSPVRYVMDPIEQLHAFEWIESNGLDLIGIFHSHPTGPETVSPTDIAEAAYAVTY
                     VILARVNDAWRTRGFWVEHGGFREVDLQIL"
     gene            23109..23801
                     /locus_tag="PL000_07405"
     CDS             23109..23801
                     /locus_tag="PL000_07405"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC88761.1"
                     /translation="MQIVFDIALILLAYVFGSIPFGLLIVKLKTGKDIREVESGRTGG
                     TNAMRAAGFWAGFATAMLDILKGAVGVWVARWLSPDQTWVHVLAPIAAILGHNYSVFL
                     IERDENGKIIRFRGGAGGAPSVGGAMGLWPGSILIILPLGMLTFFSIGIASITTMAVA
                     LFAIIVFAIRASQGLLPWAYVWYGVGAELLLIWALRPNLKKLFEGNERVVKYSLNGWL
                     RARREAKTAAGK"
     gene            23933..24469
                     /locus_tag="PL000_07410"
     CDS             23933..24469
                     /locus_tag="PL000_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88762.1"
                     /translation="MKSIDQNDISLRRWSKNDLPLLEGLLGDPAMTKHIGGPETPEKI
                     RARLERYCVDSSISKIHMFVIVLQAKQLGIGSIGFWERQWKNQTVWESGWRILPAYQG
                     RGIASKAIALVMERARAEQKHRYVHAFPSVENDSSNALCKKTGFVLLEEVDFEYPPGH
                     TMRSNDWQLDLFSHMSED"
     gene            complement(24557..25336)
                     /locus_tag="PL000_07415"
     CDS             complement(24557..25336)
                     /locus_tag="PL000_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HNC88763.1"
                     /translation="MKRIAFIILIAVLSSCNSTPPASLPTESPTPPVVTGEQIIQFSG
                     YDWIVRDGGLSGPGPNTWNGNNVWVDENGDLHLKITHTESGWTCAEVTTTERLGFGIY
                     QFQVIGPIDQLDANVVFGMFNYPTEDVGPNATNEIDIEYAHWGNPEWPIGNFTVWPAQ
                     SGVEQTSESFPVALNGSYTTHRFVWESQKILFQALHGHRDDNENEIVNWLYKPDEPLV
                     HISQQAMPVHINLWLFQGQPPVDGNEVEVVIHSFTFASLGK"
     gene            complement(25333..25632)
                     /locus_tag="PL000_07420"
     CDS             complement(25333..25632)
                     /locus_tag="PL000_07420"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNC88764.1"
                     /translation="MYAMCGRLIAKPNQRGQLVEILLRAAQMVGQLSSCHLYAVTEDL
                     ADEVTVWVMEIWDDKEAHDASLKDEHVRALIAEAMPLMGGAPSGSELKVAGGHGV"
     gene            25912..27078
                     /locus_tag="PL000_07425"
     CDS             25912..27078
                     /locus_tag="PL000_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNC88765.1"
                     /translation="MRKSVYLVVTVAILFALLTTVTGAAQAAPAAQAKWTVMVYISGD
                     NNLEDYVVKDLELELAPTGSSANVQVVALADRGPGYDISRGDWQTTKLFHVTTGMIAD
                     SASAVADWGERNMGDKQTLIDFVSWSKTNYPADHYALYFWGHGWNWHPGYVMEDVTNA
                     DTLDLDELKAALPSLGFIDVVGYDGCNMASIEVFNAWHGYATAVSSSQEYVGWDGIEY
                     DVVLAQLAANPNMTADQVAIATSQSASNDKTWSAVAVDSRFNALYTAVDQWSVALKNG
                     LAANRKKYDRAFGATQDFWQAPMDKDLYDMAYEIKRNVADTNIKNKSQAVMDAIAGVV
                     LNEHHVNGYPDAHGITIYYVSSAAGKDSNYTYYRSTVDFALTTGWDEFLDAYAK"
     gene            complement(27571..27954)
                     /locus_tag="PL000_07430"
     CDS             complement(27571..27954)
                     /locus_tag="PL000_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC88766.1"
                     /translation="MCPVRMSKVESAVRVVIEFNEAFNRHDVEGMMRLMGDDCVFENT
                     SPAPDGTVYSGREAVTQFWQDFFRESPQAHIEIEEIFGYGIRCVMRWKYSWVDVTGNK
                     GHVRGVDIFQLKDGLISQKLSYVKG"
     gene            complement(27988..28857)
                     /locus_tag="PL000_07435"
     CDS             complement(27988..28857)
                     /locus_tag="PL000_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC88767.1"
                     /translation="MNTIILSILLGIGGMFGWGIYDFLGGVFAKQIGSFRSLFWSQLA
                     GLISVLILAVVARLTVDIPILVVALSLIAAILYSAGYLFFFKGFETGNVSIIAATMNL
                     WAVFTMLFAFVFMGQRLSALKTLGVLMIICGATVASLNWNEIGNRKFQLSAGVREAIL
                     GAFFFGVFWNVSEIISEKVGWLLTTLLIKAGIIIFLSIFSFLTKQGISLTHISANTKS
                     IIFLMGVIEAGAVALVNYGLTIGDAILITPIASALSIVTITLAIIFLKDKINKIQGLG
                     IVAAIVGIIVTAF"
     gene            complement(28941..29627)
                     /locus_tag="PL000_07440"
     CDS             complement(28941..29627)
                     /locus_tag="PL000_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88768.1"
                     /translation="MNLTVDQLAIIDKQLLDGNLVAAVRELRQFTGVQLAEAKIFVDD
                     RLAYLRMTRPSQFQTAQKASPLPQPWVLQQIKPLPDEALREFRALLSHIDATLCGLNY
                     VTTLSVHAATVPASDLPIPELMMTFFPKSTPFEAKIRSAQPDELVEYVTECFRYVGGG
                     SAGPQLIDALLSEYWRHLNLLVPLETSTLYYYGSDVGLPGHYILWRFAYLLHDSKTNR
                     CVVITGNSTD"
     assembly_gap    29750..29849
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(29874..30887)
                     /locus_tag="PL000_07445"
     CDS             complement(29874..30887)
                     /locus_tag="PL000_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC88769.1"
                     /translation="MSDKQVLVTGASGEIGQALVQGLAARGGYRIVTSDFMPLPDSIK
                     HLSAEHVQGDLVYKIKTFYDYDFDIIFHLAASLSSKAEVATEEAHRINVEGTMQLLML
                     AAYRSEKYGKAVKFLFPSSIAAYGMPTLDAKRKAGLVKEDEWDYPHTMYGCNKLYCEK
                     LGMYYGKFYGQKHLDEKPPVMLDFRAIRFPGLISAFTLPSGGTSDYGPEMLHAAAQGK
                     PYSCFVRADTKISFMAMPDAIKSLLMLMDVPREKLSNTVYNIAAFALTAEEFRQRALK
                     AFPDAKINFEPNPRRQGIVDSWPEDVDDSRARKDWNWTPDYDVDKFFDEYFLPEIRKR
                     YGK"
     gene            30965..31774
                     /locus_tag="PL000_07450"
     CDS             30965..31774
                     /locus_tag="PL000_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88770.1"
                     /translation="MMFLRFQIPAKKFLLTALTTLMLTSSCAVVDAMLTTPTPQIPTE
                     TPIPTATIVWFPPSATPSAQPFSTQLPTPEMRPGLGSIVLTDNFSNEEDWNTATSDQA
                     SAAIENNQLILASQSKVYMLSLRRDLALDDYYAEITARINLCRGDDSYGILVRANAVA
                     YYRFALTCNSTVSAERVSVGTRQLLQSPLPSGDAPPGAPGEVRIGIWAVGTEMRLFLN
                     GRYQFSISDTNYPSGTIGVFVNSAGDTGTVISFTELTVRQVNAALPAESSP"
     assembly_gap    31847..31946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31947..33080)
                     /locus_tag="PL000_07455"
     CDS             complement(<31947..33080)
                     /locus_tag="PL000_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC88771.1"
                     /translation="MDWLTGSKQGEARKLISQLADSTKRDAASQSLLKLGADAVPALM
                     EALQTQDLNLLLLYQHLLARISPAQPQLIKVLSTAHPVIRGRIIEVFGFSKDKALIPV
                     LLDALKSEYFTVRAKALWALAAIGEERTVSDLLPLLKDPEDEVRIAACKAVAKFRDPS
                     TFDEITNVLLDDLKIEVRQAAAKALGDTRHPAAIPFLMEALRDSYWWYEKEQAVSVLL
                     TAIENMGEAVVYPLIDALADKEGTVRKFAAMILSKLGDIRAIEELGMALYDLHHEVGE
                     AAAEALTKFGSQAVDILIESLNHPEAAIREHAVTALGKIQDVRVAPVLIEMLHDPDRV
                     VQKKAMASLANLRDERAIPALRQIAENRADREMSALAKQLLNDI"
     gene            33302..34261
                     /gene="pdhA"
                     /locus_tag="PL000_07460"
     CDS             33302..34261
                     /gene="pdhA"
                     /locus_tag="PL000_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNC88772.1"
                     /translation="MKKEQQLDLYYQMVLIRRAEERGAELYQQGKIGGFMHLYIGQEA
                     VSTGLIAAREPRDRVITAYRDHGVAINCGISTNEVMAELLGKATGMSKGKGGSMHMAS
                     TEKNMWGGHAIVGGHLPVAAGLALGDQYAKNDNITICMFGDGATNIGYFHEALNLAKT
                     WGLRVLWVCENNQYGMGTAVERASAVTEIRQKAEGYGMKNGQVDGMDVMKVYEAAKEA
                     LDFIRKEGQPYLLEIDTYRFRGHSMGDPERYRKAEEVKQWQENDPIGIYNKYLLDKKI
                     ATRKQLDEIESRADEEVNKAVEFAEASPEPTMEDLYADIYADN"
     gene            34353..35327
                     /locus_tag="PL000_07465"
     CDS             34353..35327
                     /locus_tag="PL000_07465"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNC88773.1"
                     /translation="MAKITMREAISQALMEEMDRDPAVFIMGEEVGVWGGTYAVTKGF
                     FDKYGADRIKDTPIAENAILGAAIGAAMVGQRPIAELMTINFAFSAMDYIVNQAAKLR
                     YMFGGQFMLPLVIRAVGGGGRQLGATHSQTPDAIFAHFPGLKVVSPGTPEDAKGLLKS
                     AIRSNDPILFIEHATMYQVRGEVPEGEYTIPIGKSKIQRPGKDLTIVTYCKGLELSMK
                     AADDLSKQGIEAEIVDLRTLRPLDMEPVLESFKKTNRAIVVEEGWKSYGVGSEIVSRI
                     YEEAFDYVDAPIIRVAQKEVPLPYNRTLEQAALPQVHDIVAAAKEVLK"
     gene            35327..>35715
                     /locus_tag="PL000_07470"
     CDS             35327..>35715
                     /locus_tag="PL000_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88774.1"
                     /translation="MAETINMPKLGFDMAEGLLVRWVKQVGENINKGDVLAEIETDKA
                     TVEVESSASGVVLSHIVDQGSMVPVNAPIAVVGAAGEKVDAPAAAPKAEAKAEEKPAA
                     QTQPTAASAPSGQASAPADNGPVKASPL"
BASE COUNT         9357 a         9400 c         8398 g         8360 t
ORIGIN      
        1 ccaggcggat cgttccattc ccgctgcctg tgttgacctt aacggtatag ctactacccg
       61 aaccagtgac tgtggcaatg ctcgcccccg tcacaccgct ggtagtgagc gtaaagtcat
      121 ccttgtccac accagtaaca gcctcagaga aggtgacggt gtaatccaca ctggccgccg
      181 ctgttgggtc tggcgaaatg cggtagacac tagttacata tggagcaaaa tcaacattta
      241 taagctcaaa aggaatttcc cagccattta ccgacgcaat gatattgtaa gtgccttgtt
      301 ggttattcgc aacgaacatt ggtgaaatag catgtccatt ggcatctgtg acggcagtgg
      361 caattcttga atttgttcct tgaaaagtac cactgggacc acttgcgggt gcagtaaagg
      421 ttacattcct gccaacaatt ggctggccta aatagttttc aatcagtaac acaagaggcg
      481 ctggaaattg ggtcccgggt gtagtcgctt gtccatcgcc accaactgtc catgctccag
      541 caggagcagt aggatccagg ctcataacac gaacttcatt gttgatattc ataatcaaca
      601 tattgccatc aggtgaaacc tccatttcgg tgatactccc tccaggagat gatggcgtat
      661 aagtggatat caagtgataa tcaacagcac tgataaattt caatacatca cttccagtat
      721 ctatatcact aagaacaagc atgttttgac cgggaatgaa ttcgattaaa ttcccgggcg
      781 ccccttcaag tgcgcccaac tgagactgca ttatgccatc ccagacctgt ccactggatg
      841 taaatatttt gcttccatcc ggagctattg tgaatttggg tgctgacaca ggtccatgtg
      901 gccctgaata aagtttagta atgttatccg tgcgaacatc taacacgaaa atattgttag
      961 gcgaaaaaat ttcattggca taaacatatt gattatccgc cgaaatttcc agttctggcc
     1021 ctgtccgcaa aaggaccgga taaggtgatt ttgaaaccca ggaatgtgtg actgtattga
     1081 tgacatgcaa gtaatccgta ccgccaacgg ggtcgccatc tgaatataaa cggccgctac
     1141 gtccataaat cacatcaaat gggcggttta catagtagat actgtccgag aggtctggag
     1201 taacctcggc aattgttgca cctgttaccg ggtcaataaa gagcaatttc tcaagcccgt
     1261 tcagcgcaat agccaattca cttccatcag ggcttaagtc aattccgatt gggtttgcac
     1321 ctgagggaag caaataactt ttaacaatac tggatgtgct taggctgaca acatccaccc
     1381 tgccagattc atcagttcca tatgccaccc cgcgcggtat gtcaaaaaca aaatccctgt
     1441 agtgatgcag gcttgtcgga agtgggacag catcaccagg aggagtaacc cccaaattga
     1501 atttagccat cccacctgtt gagctgacaa acaatgtatt cccattatta ttagcgacaa
     1561 tagctcctgg agttccaacg acattagtac ttgctgaaga aagcgggtaa gagccatcca
     1621 catcatggaa atttaacgtg tctgtttcca gattaccatt gtcatacaaa accacataat
     1681 agtcttttcc aggcagggtt gctgccggtg taaccagtga atacaaagaa tttctagccc
     1741 ttggcgtcag gttggatgtg ttgtagacag caaaatttga aaccaacaag ttttcatcat
     1801 taatcaggtt taatctgatg ttttttacat aacctgtagc atacaaatgt gttctatcaa
     1861 gcagaacagg cttcgccaag ccgttggaaa tattgtattt accaaggctg tcatatccgc
     1921 tgcaacatgt tccagtaaca atatataggg tgtttttatc tgaagaaatg gcaccaaatc
     1981 tttgctcata cacttcggta tcatacaggt attgagtaac aatttcggaa tgtggactga
     2041 gagttaaatc aataacatgc aagccattac ttgaaagtgc ataaagaatc ccggtacgac
     2101 cataaataac atcagtaact ttcgtgcttg atccactcaa agcagatgga atttgcgaaa
     2161 tggaacttgt agacagattt acaaaggcca cataccctgt tccagattgt gctacggcaa
     2221 gttcatttcc atcagggctt aggtccaaac taatgggtga agtcccgtag gcaagcgtat
     2281 acgaaccaat aacagaaaga tctgtcaggt caacaacatc aatcttgttg ccatttttat
     2341 ccgccccata taacctattt cttccctcat ccactaccaa gtcagaatat gcaataaggg
     2401 aaggtccacc accaccagtc tttactattg tatatacttc tccactcgta aaagccgaag
     2461 aaaggctatt ggcacccgcg tctacaattc ctccattact caaaacatcc agacggatcg
     2521 tgccatttcc actacctgta ttgaccgtaa cactataaat gctaccagag ccgctcacac
     2581 tgcttataac cgcacccgaa actccagttg ttgtcaatgc aaaatcgttt gcatccacac
     2641 ctgttacatt ttcagaaaag ttaacagcga aagcaacaag cgccgctgac gtattattca
     2701 aaccagcacg tgtgacctcc aaaactgttg gaggaaaatc catatactca atcgaaccaa
     2761 tatcacaagc actcccttgt ggacgagata atcccaattg gtctgtcgaa ggacatccaa
     2821 ctgaagttcc agcatcaata gccgggctac ccagcattaa tgcatgagac aaaatgctcc
     2881 cgctcaagct gttttctatt gccgggtcaa cgtttaagtt gttactacca ccagtaatcg
     2941 aacagcctgt gacatcttca ataatattgt aattcgccac tgcaatggta ccggcacaat
     3001 ctggtccgga attcgaactt gagttattgg caatgatcga attggtgata ttgtatgttc
     3061 cattacttcg acgaataccg ccacctgtac cagccgtatt ataagcaacg gttgaattct
     3121 gaataaaagc agaaccggcg gcattgttcg atacataaat tccaccacca ccgctggtcg
     3181 ctttattccc agtaactgtg ctgttgacta cattaagcgt cccattatcc aggaatattc
     3241 ccgccccatg ctgggttgca acgttgttca ttaaggtact tcttgtcaga gtaaaactgc
     3301 cattgacaat gtaaacagcc ccgccattgt acgatttgga atttttgact acaaagtttt
     3361 caacattcac agtcccagaa actgccaaaa ttccgttatt agcattggcg ccatcaataa
     3421 cagatgcacc agtttgggtg gtaaatcctg agttccatcc cccggaaagc ttcaggtttt
     3481 tattgataat tacaacattg ggtgtgccat tcgaggaaaa cgtatacgtc cctgttgcca
     3541 cattaatgac atccccggct acagctttat taaccgcctc ttgaatatgc tgacatggag
     3601 ctgcaattgt tgtgcatgta ttaccgtcgt taccgccaga tatatttaca taatatatgg
     3661 tactaacaaa cggacctaca ccagatgggc caaatgaatt ttgaacatta cttccattag
     3721 gtggcaaagt tgttaccaca ggtgttggag tggcggtaat ttgacccaat gcagcacttg
     3781 ctgcatcaac ctgaaaaatt cccgaaacca aaaacaaaat gaccgccatc cctataaaca
     3841 aggcgacctt gtaaaaatat tctttttttt caaacatgaa aacctccttt ttattttcca
     3901 gcccattaag aagtggtatt aaaccatttt tcaccgtttt atgtacatta ccaccttaca
     3961 ggttttaaaa ccaatttaac cgctaatcta tcagattccc ttcaaaattg ccagtgattt
     4021 tagtcatcat attaaggggg aacacccaaa aatcttgcag aatcaaaagg actccagagt
     4081 tttgtaagcc tggagtcctt tctgttttta ttactctaca catttgtcag cgattttttt
     4141 cgtctattct cctatggttt ctccaaacca tgggccgtca atagttcttc attttccaaa
     4201 atctctgttg tcaagccatc agcgacaacc ctgccttcgt ccatcacgat cgtgcgcgga
     4261 aacaattcct gcacaagttt catatcatgt gtggagacca gcatggtaat cggcagttct
     4321 cgcaacaggt tgatcaaagt ccgtcgtgcg cgcggatcca gccccgccga tggttcgtcc
     4381 aaaatcaaca aacttggatt catggaaagt actgtggcaa ttgcaatccg cttcttctca
     4441 cccacactca aatgatgcga gagtctgtct ttgtaggcag tcatcttcac tgcttcaagc
     4501 gcggcgtcca cacgagcacg gacttccact tcaggcaagc ccatatgtag tggaccaaac
     4561 gccacatctt cgaacaccgt cggcgaaaaa agttggtcat ccgggttttg gaacacgagt
     4621 cccaccattg cacggatggc aggcaaattg tcacgagtga ggcgttgacc gcggatctcg
     4681 atttcgccat tcccgctcag gattccgttc aaatgcagca tcaaagtgga tttccctgca
     4741 ccgttcggcc caaccagggc caccttatct ccattgcaca atttcaaaga aacaccatgc
     4801 aaggcagaat gaccatcagg ataggagaaa tgcaaatcac gaacctgcat acaaagtgca
     4861 tcgcctgtct cggctttgtc attgattaaa gttttttgag tttcatgtgt aattggcatt
     4921 agagtcttcc aagaatttgc atcacaaaaa taagcacgac aaccaaagca gtgacaagat
     4981 aatctctaga cttcatctcg tgcggattca aagtatacag atgcccggta taaccacggg
     5041 aaagcatcgc attatagata cggtcactgc gctcgtagct ccgcaaaaag agttgacctg
     5101 ccatattacc cgcgaccttc gcccgccaaa caacgcttcc acccgacctc tggcctgata
     5161 ctgccgccga acgagattcc cttgcccgca gcagacggta cacctcatcc accaacacaa
     5221 acaagtagcg atacaaaaac gcgatgatcg tggtgaggat ggcgggcaca tgcaaatgct
     5281 ccaacgcgtg gatcaaatcc gggaagcgtg tgacagccac caacatgatc gccatctgca
     5341 ccgaaagcca ggagcggata agaatactta caaaccgcaa caggcccgca tctgtgatcg
     5401 tgaaatccca catcaaaaag tgaaaagatg aaacaggatt tccaggaatt gaaaataaaa
     5461 cggtaaccgc caccaatgca aagggcaatg caacaaatga ccgcttgaac gtatacatga
     5521 agcccaggtt tgagagtaag ttgattccaa gcagaaaaac ccaggcaagt acaaaagcca
     5581 gccacgcccc atctggcagt aaggcattgg agacaataaa tacaacggtc acgatcgttt
     5641 taactcgagg gtcaagacgg tgaacaaagc tttccttgtc atgataacgg tcaaaggcgt
     5701 ctgcgtgcat atgtccctgt ttctcctaac cagcattccg ctgttgaagg gagcgcccaa
     5761 gcaaaaaggc gacagccaca acaaccagca cccccacccc tccagcaaca atggtggata
     5821 ttggagtttc tccaagaaat ggaattgcat aatcggggat gatctcatag ggtgcagact
     5881 gaccactttc aatgaagccc atattgatgg ctacacgctc taatccgtcg gggtccgccg
     5941 aggcaaatgg agaaagaagt aaaacggcca gagtaatcaa aaccccagcc accacccaac
     6001 cacgcccacc ctgagccgaa gcggattttt catccagcaa atcgggtcgg gtgcggaaga
     6061 tgaatcccaa agcagcaacg gttacaaggg cctcgccaag accgatcaaa gcatgaacac
     6121 tgagcatggc tggaataact aaccgcaaat ctgaagtacc gctcaaccat aattgaagtg
     6181 aagtggccaa agcaccagcc attacagaaa gccacgcagc cacaccggca acagcaagct
     6241 gaacgccacg ccccttaccc aacacactac ggtacaggcc gtatcccacg cctgccgtaa
     6301 gcagacccat atttaaaata tttgccccca tggcaagcag gcctccatct tgaaatagaa
     6361 gtccctgcac ggtaataacc gctgtcatca ccaagatccc agcccagggg ccgagggtaa
     6421 tggctgccaa tgcaccgcca agtaagtgtc cggaggtacc gcccaatacg ggatagttaa
     6481 tcatctgagc cgcaaaaatg aaggcggcca tcacacccat caatggaacc tgtctttcgc
     6541 caagagattt attcgttttt gaaattgcaa ttccaagggt gatcacagta atcacccagc
     6601 aaatgatgga aaccaaaaca ctaagaaagc catcgggaat atgcaaaggt gcaggagagt
     6661 aaaacatatt attattttcc tttctgacag ctgggacaaa ggccaaaaaa agtgacatgg
     6721 ctatcggtca gcttatagcc gctggtggat tcaagttttt ggtaaaggct cttcaacaag
     6781 gcatgttcaa cctccatttc atctccacag atacgacaaa ccaaatgatg atgttcatgt
     6841 cgggcgatct cataaaccag atgaccgttg cccatatggg ctgggcgcgc cagtccgttt
     6901 tcagcaagaa actcaagcgt gcgataaaca gtgggttcgg tgagaccagg caggtctttt
     6961 ttcgcagctt cataaatttc ggttggggaa agatgagtac ctgcatgatg aagcgtatgc
     7021 agaatcgcca ttctttgcgg ggtcatgcgg tatccacggg cacggagttc gggggtatat
     7081 agatcggcac aggtcatagg tgtccttatt gcaacttttt gcaataatgt atagaggagt
     7141 acaagaaagg aattcttacc accctaaaaa atccgctctc tttcatttat tgccacactg
     7201 ttcaaggaaa attggcgaaa atccgcacaa cacctgcact gtgccacatt gcgttcgcgg
     7261 aaaaaattct tgaggcagaa gtagttgaac ctcaagaaac ttgacctaca ctgcaggtaa
     7321 cgggaaaact acctccagcc ttctaaaaat acttttacca gagccgttac ggttcccaaa
     7381 catagtgaac tagtgcggcc agatatggct tgcctgtgat ggtatccatg ccaacggccc
     7441 aggtttgcca atccattccg ccaaattcat cggtaccggg gaagtagatc gaataaaaat
     7501 tcatatccgg gtagggccac tcgggaaagt aggacgcatt cccgatctgt aattgaccgc
     7561 acacaatcag ggaagcaggt gtgaacacct tttgcagaga cggcaaaacg atctctcgaa
     7621 acgtgccaac tgtcggttgg ccgctgccga atgaaatgcc ccaatcaact tcagcggtat
     7681 cttgaaaaat ggatgccgta tccagtgtgt aattgacaac cttgtcattg cgatagtagc
     7741 ggatatccac accaattgcg ggggagacaa gcttttcaaa agcggttcca ttttcctggg
     7801 taatggcttg tatcaattct tcgatcagga tgtagccgcg attatcggca cagaaatcat
     7861 aaaattggac aggggtgcgg atttcgttga ttgccagtgt aggggtggga gaatatgtcg
     7921 gtgtagggtc gggggtgaat gttgcgggga gggtcagggc tggttgaggt gtggcagttg
     7981 tcagcgcggt gggaggcggg gcttcatcca caggaagagt gcaggcggta agggtgatga
     8041 gcacaagcaa gtaaaaaagt ttcttgaagt ccattctgtt tcctttttcg atggtttagc
     8101 tcaagacgaa aaactggcgg ggggtccgcc agttgattgt atttttatga agccgctgcg
     8161 tcctcggcca ttttttttgc aaccctcttg atcacttctg ccacaccgcc gatggggttt
     8221 ttgagggtct caatcgccac ttcaaggata tcgggggcca tgcgtttgag gttgcggaaa
     8281 cggcgggcca gaaaagtttc atcgggttct tttgctttga gcgcaacttg aatttcctgc
     8341 aattccgcct tgatatcttc cttatcatct ggtttcaagt tgggctgctc atccaccatt
     8401 tgttccatgt ccttaaagaa agaatccaga ctggttgcag aaagtgaaac tgtattgcca
     8461 tcgcccatga caatattgct attattgaca ttgccgccaa tgacaattcc ataatcacca
     8521 gcgctcaggc cttctttttt atttttttga gtcatgctgc ctccatcatc atcaataaac
     8581 actttgtgga cacagggtct attataaatc cttcggagca atgacaacac ggaagccgag
     8641 atcatcattg aacatcaacg ggatggacca gttacagaaa gaggaacgtg caaaccattg
     8701 atatccgatc catgagccgc cacgcacgac tcggtatttt ctatcctcat cataccagga
     8761 ggaggtccac tcccagacat tgccgcttgt atcccacaca ccattcgggc ttacacctgc
     8821 agggaaggta caaactgcgg tcgtaccgcc aaggcctgaa ccttttgtat cgctatccca
     8881 ggtattggcc gccgctttat cgaaaccttc tccccacgga tattcgcggc catcttcacc
     8941 gcgtgaagcc cgctcccatt caccttctcg cggcaagcgg atggtgtacc cctcaggcac
     9001 agcaacgatc ttctttgcca gccagttgca ataggcttcc gcttcgaacc agcacacccc
     9061 caccacaggg acaatgggat tgcttaactc aatgttgtgc cagtagtatg ggatattacg
     9121 ctttgcaagc ggacgatgtt ccaaccaatc atgcgccacg gcttcgagcg tacgagaatc
     9181 atatttaccg gtgcgccact cccagccatc gtagctccaa tattcctgtg tatgatagcc
     9241 gtcttcttta acaaagcgtg caaattgaat attggtgatg ggatacttcc caatgaagta
     9301 attgtacggg atcgtcttcg gttccttctt aatccctgcc tgatactcac cttcaggaat
     9361 ttccgcaaac cgatccaggt caccgggccg ccagcccagc ctgccgagca aaagactcgc
     9421 atgtcttctc aggtcaggcc gaatggcgcc atcctgcatg gtatccacca aagcctgaat
     9481 gacgtattcc ctgttttgtg gggagacccc gcctggccag gtatccaata cggcatcgcc
     9541 tgccagcacc accgcttcac cgggaggccc gggttggttg ttgaccagtg cttcaacgac
     9601 ctcacctgcc accttcggca attgctgacg aatgccaagg tacccgatgg tcaacagagt
     9661 tacttcgcgc caagcctgct cgcccacgtg cgctgacaaa acttcgatga tcggagcagg
     9721 gttgccttgc cccatcaatg ccagtgcgac agcggccaga tattcttcaa aggtcaggtg
     9781 gataaaacca tattcaccgg ggccacgctc aagcaaaagc gcagcatgtt cgcgcacgtc
     9841 aataaggaat tgacgcgccg cctgatgagg tgaaacgtcg ccgcgctcta aaaacagttc
     9901 ttccaatttg cggcgcatgt cttcacgccc caccaaccca acaccagggc tgacctcatg
     9961 catccacaaa gccaacgggg ccaaaatacg gacggtctgc aattcatcaa tgtcacgacc
    10021 gggggcacgt ccgcttaaac tacgggcacg gttccaggtt gaaagcaggg tgttgacata
    10081 ttggtcgtac agttgaacac ggcgctcagg cagggaaacg ccctgccgct tcatcaatgc
    10141 cagaatggta aggagcaagg gtgtggaagc cagctgccta acgccgggat tctgctgaat
    10201 ggcatcgagc agttcacggc ggtcggtttc cgcatctgca cgggcgaccg cggtatttcc
    10261 ctgagcttgt ttttcgagag cggtcgtcca atatttgacg aattcctcga tctcatcctc
    10321 ttcgaaatcc acaatggtgc attcgacaag gtcttcagca gaagggcgga cagcacgata
    10381 ccccaccaca cggctggtta agacaaactt attaccttca cgacgatgga aggcgaaaaa
    10441 gtccaccaca cgctccacaa ccgtattgcg caaactgata tcacgcactt catcgaggcc
    10501 atccaataaa atgagggcgc ggccagcctt caaagcttct ttcagcatcg ggccaacggg
    10561 caggtctgca ccaatgccgt caaaatattc tgcaataaaa tcatcaaggc gaacatcacg
    10621 ggcggtgatg gcattggcaa aagccgccaa cggaaggagg atgggcaggt aatcgttcag
    10681 gccgatcttc ttcccttcgc cgcgtgcaag gcgcaacgcc agatatttta caaaagtggt
    10741 tttgcctgcc ccgggatcac caaggatgat cacccccgaa tgtttttgca taactttcag
    10801 cagcggcacc ggctcaccga ggttgaacat ttcctcttct tcagcggata cgttacgtcc
    10861 agccaggctc acatcacgct tccaggtttc gccttcgggc aattccatgc gggccttcag
    10921 cggaacataa agatcgagca gtttaagctt gaggggaacg cggtcagaaa ctcccatccc
    10981 tttcaggctg agatagtaat gacgatccgt tagataagcg agatatgctg ctgtggcttc
    11041 acgtaatttt tcgggaggca gggcaggttt caggtcacgc cagaaacggt ctgcgagaac
    11101 gatggtttcc ccatgaatga tggcactatc ggttaccgat ccccctactg caatgctctg
    11161 gtctcccgcc tgaatttcct gctctggttg tgatggcttt gatgtctttt tagagggttg
    11221 ctttttttcg gtcattgccg cctcgttgat ttatcgcttt attccaaatc gagtaaacac
    11281 cgcatggaaa ataattcaag ctttccgttt accaatttat gcgatacttt gtaaaattca
    11341 gtatacagta attcgaaaga tttctgcttt accaaattgc aaggtgaccc actttattga
    11401 cattttcact gttctgaata taatcagcca aaatattgaa ggcgttgacg aggaaaagta
    11461 ggcttgtacg acggttcaga gagcaggtca gaacatggtg aaagacccgc ccgaaacaac
    11521 aaagccgaat ggtcctccga gctgcaaagg caaaaaatca gacagtttga gtagcctttg
    11581 acggaatgct cccgttatag agctagggcc tggtttttat aaaccgtgcc cgaaagagtg
    11641 aggctggctt cccgttgcat cataacggga ttttttgtaa ggtaatgcca ctatgtcagc
    11701 ctaatttggg tggcaccacg gatgcgagac catgcagcgt tcgtcccagt tgggatgaac
    11761 gctgtgtttt tttaacccga ccgaaggaga gaaaaatgaa ccccgtgacc gagcaagatg
    11821 agcaaaccac atacatgcaa gaacgtgcca ttgaaacccc cactgacgac tcccttcccg
    11881 ccgcacaggt gatcgaagag cagaataaag aagcagagca aaactatacg ccgcgcttta
    11941 tcccataatt cattttttgg aggaacccca tggaaaatac cacccgcttt cttttgaacg
    12001 aaaccgacct gccaaagttc tggtacaaca tcgcggcgga tagcccggtc gcaccgaacc
    12061 cggttctgca tcctggaacc ttggagcccg tcaccccgga ctttttgtct gtgcttttcc
    12121 ccatggacct gatcatgcag gagatcagca ccgaacgcta tattgagatc cctgaagagg
    12181 tgcgcgaagt ttataaactc taccgcccca ccccgctcct gcgcgcccgc aggttggaaa
    12241 aggcgcttga taccccggcg catatttatt acaaatatga aggcgtctcc ccttcaggaa
    12301 gccataaacc aaataccgcc atcgcacagg cctattacaa caaaatcgct gggaccaagg
    12361 cactgaccac tgaaacaggt gcaggccaat ggggttcctc gcttgccttt gcctgcagtg
    12421 tcttcaacat ggaacttgag atctacatgg tgaaggtgtc gtatcaccaa aagccctatc
    12481 gccgcaacct gatggaagta tatggtgcca ccgtgtacgc cagcccaaca gaccgcacca
    12541 attatggccg ctctgtgttg gcagagaatc cagacaacaa aggctcgctt ggcatcgcca
    12601 tttcggaagc tgttgaagcc gcagccacct cgaatggcac aaagaaatac agcctggggt
    12661 cggtcctcaa ccatgtgttg ctgcatcaaa ccgtcatcgg tgaagaagcg ttgaaacaga
    12721 tggacatggc aaacgaatat ccagatgtgg tcatcggctg tgcaggcggc ggctcgaact
    12781 ttgcaggcat tgccttcccc ttcctgcgtg aaaacctgaa gaacggaaag cgcgcccgcc
    12841 tcgttgcagt ggagccaaca gccactccgt cactgaccaa gggtgttttc acctttgatt
    12901 acggcgatac ggccaagatg gcacccatcg tcaagatgca tacgctcggc cacgatttca
    12961 tgccccctgg cattcatgca ggtggattgc gctatcatgg catggcgcca agtgtcagcg
    13021 ctctggttga tgcgggacac atcgatgcag tagcggtcaa acagcgcgag accttcgaag
    13081 ccgccatcca atttgccaat gcagaaggca ttgtgcccgc ccctgaatct gcgcacgctg
    13141 tccgtgcggc gatcaacgaa gccatggatg ccaaagccaa gggtgagaaa cgcgttattc
    13201 tgttcaacct ttccggacat ggcaatttcg acatggccgc atacgaatcc tatctcggtg
    13261 gaaaactcga agatttcgag tatcctgccg aggcggtgca ggaagcgatg aagaaacttc
    13321 cgcaagtgtc actctaatcc gttaaaaaaa catccccatg actttttgag tcatggggat
    13381 gttttcgttt tgcgtttaac tattgtgaac aggttcgcca aataccacta caacaatatg
    13441 ttgattaaga aatttcggcg ttacattttc gctggaaacc acctgcccaa gggtatatcc
    13501 acccgcaatg gggacgttcg tcccaagaat gtcgcggact gcctcaatct ccaccccggg
    13561 gtgtgctttc aatagcatct gccaggcaac atccaccagt acaagagcaa agacaggttt
    13621 tgaatcacca agttgcagca gggcttgttg ggtggcatca cgcgctgcac gttcacaagc
    13681 ggcacggctc ccaaccaaca agtatgcatc gatcccatca cggatggatg cattcatgcg
    13741 aaaacttcca tcagcctcaa cgcggatggg agaacgtgta atcaaatcat cgcccacttc
    13801 cacaccaagc ggatatagac gcgccagata acttaaagga gggaaagccc aatcacgggc
    13861 cgggtatcca aagagagccg cataggcttc tgacgctggc ctgccatcca atgtccgcag
    13921 ccagaaacca cgcgaacggg tcactcgaaa ttgttttcca acttgatccc aaccatggcc
    13981 atacccaaca ccggtcttga tttcaccgcg taagaaagcg gccaccatac cgccggtacc
    14041 cgtttgtgtc ccggtcattt ggtaggtatt gccagtattc aaatccccgc ttgagagtgc
    14101 tcccagtatg ttcaaaccag gcggcattgc tccgcaaaat tgttccgcat ctccattgaa
    14161 accatctgca aaaaccagga gggactgacg ctcctcccgc gctgcggcat gatcagccac
    14221 tttagcagcc gtctcacgac cggattgagc ataccccgga agccacatgg catctgcttg
    14281 aaaatcgccg ctcaacaaag caaccactac agaatgctga aaatgcccct cactggttaa
    14341 ccctgcaggt gaactaaacc ctaccatggg aagctcaccc agcaggctgg tgacgccgtt
    14401 caaaacttca cgcgcctggt attgatggga tgccaccacg atggcaaagt tggctgaatt
    14461 tgcgccaagg tgattcaacg cctgatgcgc cgcctgtaac ccagcctcac gcccatccaa
    14521 tgcctgggca gatccaacgg atgcaaacaa tgccataacg cctcgctact cttccgcaac
    14581 cgggatggta atgtggaatg tagtcccaac atggaattcg ctttcaaacc atatactacc
    14641 gccttgcatc tcgatcaggt tctttgtgat attcaatcca agccctgtgc cggatgccac
    14701 ttccttcgaa tactcggtgc ggaagtattg ctggaagacc tttttctgat cttccggggt
    14761 catccccaat ccgttatcct tcacccagat atgcacgacg cgcatggaac tgtttgtctc
    14821 tccgagatta ctcgcctctt ctgcgccaat ctgcaagata ccgctttcag gcgtatattt
    14881 gtaggagttg ctgagcaaat ttacaagcac ctgggtaatg cggttggggt ccgcccaaat
    14941 gacgggaagt ttttcgggca gcacaatttc cagggtttgc tttttctctt cgatctggcg
    15001 cttgagagag cggactgtat catcaatcaa aggcggtact tctgtcactt tataagcaag
    15061 gctcaaacgt cccgcttcga tcttcgagat gtcgttgagg tcggacacca ccgtatccat
    15121 gcggatcaca ttggtcttga tcgtattgag aaaattcgcc tgcatgtcac tgattggacc
    15181 taccgctcca gccgccatca gatcggtgta ccccttgatg gaagtcatgg gattcttcaa
    15241 ttcatgtgcc acaaatgaaa caaatctgct cttctcaagg ttggcacgct gaacctcacc
    15301 aaacagctgc gcattcgaaa tggcgatcgc ggcatggtcg gtaagacggg ttaggaagcc
    15361 aatatccact tgtgaatcgc tgatactttc caaatgaaga agaccgatca ccgtcgtttc
    15421 gcggcggatc gggatgatca tttgggtgtg cgcgctggaa agcagtttgt cgtttgaggt
    15481 ggcaacagaa agactttgag gttgtccatt ttcaatggat tggatcatgg caggcagttc
    15541 gattttcatc gcctgatctt cagggaggtt ggccatacga tcatccaaac cctgatgagc
    15601 catcacccgc agtttgcctt cttccaacat accgatcaac cctgcctctg catccgattg
    15661 acgcaaagcc cactccagag tgatgtgcat ggcgcggtcc atttcaagac tggcattcaa
    15721 ttcacggtca atgcgctgca tgactgaaag ttcttccacg cgggccgcaa gttcctgatc
    15781 ggtcagggtg tataaacggg cattttcgat cgccaccgcc gcctgacctg caaacgcggt
    15841 cagcaaatcc tgatcgccgg ttacaaatgg aagtccgtcg cgacggttga tcacttcaat
    15901 gacgccgatg acgcggtctt tcacctgcag aggcacagcc atcaaagaac ggcttacaaa
    15961 tcctgtttgt tgatccacac cgcggaatct cgaagcggaa cgattttctt cattttcgat
    16021 cacaggtccg cgtgattgca ccgcacgacc cacaatcccc gtccctggcg gcaggcgttg
    16081 accaataagg tttgcagcca ccgggcctac ggttactttg aacaccaatt ctttggtctg
    16141 ttcatccacc aggaataaac tacccgcttc acagttcaaa attccaacag cattctcaag
    16201 gatgttttga agcagcgggc tcaactcaag cgttgaggtc aactggcggt tgatctcatt
    16261 cagcgttgaa agttgatggg cgcgttgttg ggtctcttcc agcaaacggg ctttgacgat
    16321 cgcacccgcc gtctgatcgg caatggcttg caaaagttca agttgtccgc gcgtataagc
    16381 tgtagcccca tcacggctgc cgatgctgag cgcgccgatg gattttgcac ctgcattgag
    16441 agggacgccc atccaggcaa agacattttc cacgatcggg gtaaggctgc gtgcctgaca
    16501 ctcgtgaatg tagtcctgtg tcatgatctg acggcttttc gcgatcactt ccggactaag
    16561 gcctgctgtg accggaaacg gcagattttc tctttcggaa atacgctcat tattttccac
    16621 acaaaagcca tagtaataat aatcgctggc ttcgttatac aaagtaatat gaaaatgtga
    16681 agtagggatg atctgcgcag tttgggcgta gatcagttca agtacatcat cgaacgtgag
    16741 ggtcacgtta acaccctgcg aaacgcgggt cagggcattc atttcctgaa tctgacgctc
    16801 aaggcgggcc acggtctgca tacgctcaat cgccaccgag gcctgatcgc aaatattttc
    16861 aaggaatctc aagtcgcgcg gattgtaagg ctgccccgat aaacgcggtc cgagtgcaag
    16921 ccagccattg atgcggttct tgccgggtaa aaccacaaag agacgcgcac ccagcaaagc
    16981 caggcgcgat tgttctgatt gcaaatccga tggaagtgcg gcggttgtat ccagatacaa
    17041 aggcagatgt tctttttcga aataccgcac aaatggactg gtcgttgtaa aacgaatatc
    17101 gctcgtaggg cgacggtctt caccaggtag agcagagtaa aagtcgttga gagagtcgta
    17161 tgtataaatg tgaaccctgc tcggggtcag tgttgaagcg atctgctcgc gcaggatcgt
    17221 gctgatcgag tttagatcca gcgcagtggc aagcttgtgg gaaaaatctt caagtttttc
    17281 agccagcgca cgctcacctc ggaagaaggt gttatccacc acagattgca aacgcacacg
    17341 aaaaggctcc agcaataaag ccagcaacaa cataaaccca cctacaaggt agggattgct
    17401 cacaagcgca tcagaactgc tggagttaac taaaagcccc accccggcca ccaccaaccc
    17461 atacccgccc atgacaaatg cggtcaggaa aaagtacatc agtccgcgcc gcacaaaatc
    17521 gtcagtgcgc aggaagcgga aacgtaaaat ggtatagcca atggtcagcg gaaagaaagc
    17581 aagcggaaga aacaggtagg gtgaaaaatt caaaggctgg gatgcagatg agccagtctc
    17641 tttccacagc agttgaaaag cattcaagcc aagccaaatg cctgccggaa caaacgccag
    17701 catggctcct aaaaggatgg cacgcgcttg agttttcaca actggagatt gtgcagaaac
    17761 agcctgatac aggctaaggc caatgtaaaa aataatgctg atggcattga agccataaat
    17821 gtattgccag atcggaatat aggctgtggg tctttcaaaa ttgaacaagg ttgtgaggtt
    17881 gaacacagcc aacacaattc caataataag cccgacccag cgcaagtatg gacgattaat
    17941 caccacacgc ggttcaagcg ggaaggaaag gcccaggtca aatagagcgc cgcccatgat
    18001 cgccagagcg aaagtccaga aaactgtaaa ttcgtgggtg gtgaccaggt tgaaatatgc
    18061 ccccgtcaca atggcaaggg atgaagtaaa aagagaaaaa gcgcgtcctg ccggttcatt
    18121 gcggcgcaaa ccaaagatcc accagctggc aatgataaaa acaagaccta gaatggaagg
    18181 gacaataaaa tacgtggtac ggctggaggc agggaaatta tgtaaagtga cacttacatc
    18241 acgagactga cccgattctg atttcaccgt tacagtgaca ttttcaccgg gcacatgccc
    18301 ctgcaaaatg gaacgaacct cacgtgtatt acgcactggc gttccgtcaa ttgcaaccat
    18361 ctgatcgcca actacaacct gcttgaataa agcccactcc ggcattgcct cacttgggcc
    18421 tgtgccattg aacaccatgg tatgttcata caatgccccc aaaaatggct gttgaaacca
    18481 ccgaaccgac aggtacatgc caaccaaaaa aataatgaca gcaatcgcct gatatgcaaa
    18541 cacagtccat tgcaggatct ggttgaagag ggtgccattc cgcggaaggt tgatctgcgc
    18601 actgcgctga aaatttcgag ccgccatagt gaacgaattt tactctacaa cggctttgac
    18661 agtatatggt actttcataa caaagaagaa aggctgataa gaaaagagac cgctaaatgc
    18721 ggcctctttt tgaaagatca cttctttaat tgagattaaa acacccgctt caccccgacc
    18781 ccgaaacggg tacgctaaat atcgagattc ctcacgctct tggcatgggt ctgaataaat
    18841 ttcttacgtg gatccaccgc atcacccatg agcatatcaa aggtgcggtc tgcttcagcg
    18901 gcatcatcca cagtgacaag aagcagggtg cggttggtgg gattcatagt cgtctcccac
    18961 agctgctcgg gattcatttc acccaaaccc ttataacgtt gaatgccaac cttatcgctg
    19021 ccgatctctt tgaccaattt atctttttcc ttgtcgctat acgcatactt gacctgattc
    19081 ttataagcca cacgatatag cggaggttga gcaatataca aatgcccttc gtcgatcaaa
    19141 agcggcatat agcggaagaa gaaggtcaac aacaaagtgc ggatatggct tccatctaca
    19201 tcggcatcgg tcatgataat gacacgacca taacgcaaac cttcaaggtc aaagttatca
    19261 cccaccccgg ttcctaaagc agaaataagg gagcggactt cattgttacc taaaatttta
    19321 tcgaggcggg cgcgttcggt gttgaggatc tttccgcgca atgggaggat tgcctgaaag
    19381 tggcggtcac gtccttgctt ggcggagcca cctgccgagt caccttccac aatgtaaagc
    19441 tcggtcttgg aagaatcacg ttcagagcaa tcggccaatt taccgggcag tgtcagcgat
    19501 tcaagtgccg atttgcgaat aacaagatca cgcgccttac gggctgcatc gcgtgcgcgc
    19561 gccgaggtga gacattttgc aatgatggcc ttcgccgcct gcggattttc ttcgaggaag
    19621 gtgccaaaag tttcgccgac cacctgagtc acataggtct gtgcctcggg attcatcagc
    19681 ttgaccttgg tctgcgattc aaattgtgga tcggggtgtt tgaccgaaac gatggccgtt
    19741 aacccttcac gagtatcatc gccagagaaa ttcgggtcgg catccttgag caggccattc
    19801 ttacgggcat aatcgttgat gacgcgtgtc agcgaggaac gcagccccgt caaatgagta
    19861 ccgccatcca cagtattgat ggtattggcg aacgaataca ccgattcggt gtaggcatct
    19921 gtatattgaa tagctgcttc aataccgatg ttctcgatct ctttttccac gtgtaccacg
    19981 gggtgcaggt tttcacggtt gcgattcaaa tagcggacaa atgaagtcag accgccttca
    20041 aagtagaagg tcatttcacg gtcagcacgc tcatccacaa acttgatggt gatgccacgg
    20101 gccacaaaag acatttcacg gaaacgctga accaatgtat cgaagcggaa atcaatatct
    20161 tccgtaaaaa tctgtttatc aaagcggaag gtttgcttgg tgccggtctc gtccttcact
    20221 ttgccgatct gtttgatgga tccttgcggc acgccacgct tatattcctg gcgccacaat
    20281 ttgccatctc gtttgatctc ggtaaccatc cattcagaga gggcgttcac agcactcacg
    20341 cccacgccgt gcaaaccgcc agagaccttg tacccgccac cgccaaattt accgcccgca
    20401 ccgatcacgg tcatgaccac atccaacgtt tccattggtt tgccgttcgc atcttttttg
    20461 gaggggtgcg ggcctacagg aataccacgg ccattgtctt caatggtcac actgctatcg
    20521 gggtgaatgg tgatattgat tctgttgcag accccagcca gcgcttcgtc gatggagtta
    20581 tccaccactt catatacgag gtgatgcaac gctttgatat cggtaccgcc cacatacata
    20641 ccggggcgct tccgcacatg ctcgattcct tcaagtgcct gaatggaact aacatcataa
    20701 ttaattttct tttctttttc agccattaca acctccaaca atttttattg cagaacggaa
    20761 tgccgtcgaa ctatgccttc gtaacggcat tcggacgtaa tttctcgatc acagcctgta
    20821 accaggccgt catatcacga taatagacaa aactcgaagc cagcaatgcc gtggtcacaa
    20881 atgagtgacc aaagatgcca agaagggtca tccacgaatc ttcggggggg atgctccaca
    20941 gaaaattcaa cccaacggac aaaaggaaaa cggtcaatac gaacatgctg ctggtgggca
    21001 gggtgaagcg tgtcatctgc acgctgctca aaatggaagt gaggaagttc tgacgcctga
    21061 gaaaaatccc atgcggccag aaaaagaaag gcacgatcac ccacatcgaa atgaggctca
    21121 tgaacaggat cagcaggttg gcaaaaaagg cgctaaattg caaaaccact gccagcacca
    21181 aaaaggcagg caccaaaatg atcacggaga gaatgctcca gatgatcgaa gtcagcaccg
    21241 tttgtacaat ggcacgagaa atctgaatgg gctgattttc tttatcggca acggtcagcc
    21301 aggcaacact gcgaaaatac aagccgcctc caacccaccc aagcaggttg agcagaaaaa
    21361 cccagcccat caggttgaca gcatccacct gtaaaaccag tggatttccc aatggtgtgg
    21421 cggctggctg ttgtggaaat aggaggcttg aaatcccgat gggaagcgtt cgaacgatcc
    21481 aaaacaggtt gatgttcgga atcgtcgtct cataccaggt gaggatgcgt tgaatatcct
    21541 ccgcagggac accaccggtg cgccagatcg acacgacgtc atccttgatc gagttgaaca
    21601 gggcatccat gcgcaggcgt ggaccaagcc acaggaacag attcaacaac agcggcaata
    21661 gaatggccgt gatgtgcgag gcgatcatat cgaagcccgc tttgattgaa ttgatgattc
    21721 cagggggcgt gggcagattt tctgtacgtg aaacttccat aacaggttga ttctaccata
    21781 cccctgcttg cgcttgcgtg cccccacagg tacaatcttc acatgcaaga gcgccattcc
    21841 tacctgccat cagcggaccg tgtgggcgta ttgatcgcct cggtcctgct gacttttgcc
    21901 ctcacccgcc tcattcagtc gccgcaattt acgttgacga tcgaactgcc tggcttttat
    21961 tttgcatatc cgctcaccct tggcacagcc atgaccatcc tcgctgctgc actggccgcc
    22021 gcaggcatgg attggttgac tcgtggtcac cccaaccttg gggaaaaacc gaacatcgaa
    22081 catctcatgc tgcccaccct cactacgttt gtgatcggct cgccgctcgc gctgctgccg
    22141 gaaagcattg cctggtgggt ggcgctggca tttgcagcaa gcctgctggt gatcgtgttc
    22201 ctggcagagt acatcaccat tgacccatcc gcgccctcat atgcgttcgc tcgagccggt
    22261 ctgacagcac tggcctacgc cctgtttctc atccttgcca tgtcgctgcg tttttccggg
    22321 gtgcgcatgt tcctgctttt cccggtggtg ttggttgtgg cagggctgat cagcctgcgg
    22381 attttgcatc ttgatggcac tgaccgttgg gattttccgt gggcgcttgg cattggcatt
    22441 gtctgcgcgc agatcggagc agggttgcat tactggccgc tgaccccgat acagtttggg
    22501 cttgcactca cgggtccgct gtatgcattg acgatgctat ctgccagttt gaccgagaat
    22561 acgccgttac gccgtgccgc agtagggccg atcttaattt taggcctgag ctgggtttcg
    22621 gcttttttag tgaaatgacc accacacatt tcaaatgaac tccctcaccc tgacaaatga
    22681 acaacttcaa aaaatgatcg accatgtgga ttctcatgcg ccgctggagg catgcggtct
    22741 tttggcaggg aaaggctcca cggtggaggc tgtgcttgtt gtgcggaatc aggcccaaag
    22801 tccagtgcga tacgtgatgg atcccattga gcagttacat gcctttgagt ggatcgaatc
    22861 caacgggttg gatctcatcg gaatctttca ttcgcatcca actggacctg aaactgtttc
    22921 gcccaccgac atagcagagg ccgcctatgc agtgacctac gtcatcctcg cacgcgtgaa
    22981 tgatgcatgg cgcacccgcg gattttgggt ggagcatggc ggttttcgtg aagtggattt
    23041 gcaaatcctc tagcaacctt atttcatttt ttgtgtttta tgattacaac ctaattggag
    23101 gagtttcaat gcaaattgtg tttgatatcg cgcttattct gctggcttat gtttttggtt
    23161 cgatcccctt tgggcttttg atcgtgaagc taaaaactgg aaaagacatt cgtgaggtgg
    23221 aaagtggccg cactggcggc accaacgcga tgcgtgcggc aggtttttgg gcggggtttg
    23281 caaccgccat gctcgatatt ttgaagggtg cagttggtgt gtgggtggct cgctggctaa
    23341 gcccagacca aacctgggtg catgtgctgg cgccgatcgc agctattctg gggcacaatt
    23401 attctgtctt tctcattgaa cgcgatgaaa atggaaagat catccgcttt cgcggtggcg
    23461 caggtggggc gccttcggtg ggcggcgcca tggggttgtg gcctggttcg atcctcatca
    23521 ttttgccact cggcatgttg acctttttca gcatcggcat tgcctcgatc acaacgatgg
    23581 cggtggcatt gttcgccatt atcgtttttg cgatccgcgc ttcgcaggga cttttgccat
    23641 gggcttatgt gtggtacggg gtcggcgcgg aacttctgct gatctgggcg ttgcggccaa
    23701 acttgaaaaa attatttgaa ggcaatgaac gcgtggtgaa gtacagtttg aatggctggc
    23761 tgcgggcaag gcgtgaggcg aagacagcgg ctggaaagta gaaagtggaa ggtagacact
    23821 ggaatcaaaa acgtcctttt ctgaagggcg ttttttgttg caaggggtat gctatacttt
    23881 ttatcgccta ctgggaaata atcggactaa ttttattcaa ggaatgaaca agatgaaatc
    23941 cattgaccaa aatgatatca gtctccgtcg ctggtcaaaa aacgacctgc ctttgcttga
    24001 ggggttgttg ggcgacccag ccatgaccaa acacatcggc ggaccagaga ctcctgaaaa
    24061 aatccgcgcc aggcttgagc gatattgtgt agatagcagc atcagcaaga tacacatgtt
    24121 cgtcattgtg ctgcaagcca aacaattggg catcggttcg atcggatttt gggaaaggca
    24181 atggaagaac cagacagtgt gggaaagcgg ctggcgcatc cttccagcct atcaaggcag
    24241 gggaattgcc tcgaaggcca ttgctttggt catggaacgt gcccgtgccg agcagaagca
    24301 tagatatgtt cacgccttcc cctcggttga aaatgactcg tccaatgctt tgtgcaagaa
    24361 aacaggcttt gttctattgg aagaagttga ttttgaatat cctccgggcc atacgatgcg
    24421 cagcaatgac tggcagctgg atctcttttc gcatatgtct gaagattgaa gcgtgtaccg
    24481 aactgggtca gcttctgaaa agcacaaatt tatccttcaa gacgataaac gagcagtacc
    24541 gtttcttaca ccactgctac ttccctagtg aagcgaatgt aaaactgtga atgacaactt
    24601 caacttcatt tccatccacg ggcggttgac cttgaaacag ccacaggttg atgtgaacgg
    24661 gcatggcttg ctgggaaatg tgaaccaagg gttcatctgg tttgtacagc caattgacga
    24721 tctcgttttc attgtcatca cggtgaccgt gcagcgcttg aaaaagaatc ttctggcttt
    24781 cccacacaaa gcgatgagtg gtatatgacc cgttcaatgc cacagggaag gattctgagg
    24841 tttgctccac accgctttgg gcgggccaga ccgtgaagtt tccgatcggc cattcggggt
    24901 tgccccaatg ggcatattcg atgtcaattt cattggtggc attgggacca acatcttcag
    24961 ttgggtagtt gaacatgcca aagactacgt tggcatccaa ctggtcaatc ggcccgatga
    25021 cttgaaactg gtagatgcca aatccaagcc gctcggtggt tgtgacctct gcacaggtcc
    25081 agcccgattc ggtgtgggtg atcttcaaat gcaggtcacc gttttcatcc acccaaacat
    25141 tgttcccatt ccaggtattt ggcccgggtc cgctcaggcc gccatcacgg acgatccaat
    25201 cgtagccaga aaattgaatg atctgctccc cagttacaac aggcggagtt ggagattcag
    25261 tgggaagcga ggcaggcggg gttgaattgc acgagctcaa cacggcaata agaatgatga
    25321 atgcgattct cttcataccc cgtgtccacc agccactttc aactctgagc cgctgggagc
    25381 accgcccatt aacggcatgg cttccgcaat caacgctctg acatgctcat ctttcagcga
    25441 ggcatcatgc gcttccttgt catcccaaat ttccatgacc cagaccgtta cttcatcagc
    25501 caggtcttct gtaacggcgt ataaatgaca actcgagagt tgaccaacca tctgtgcggc
    25561 gcgcaagagg atctcgacga gttgcccgcg ttggttgggt ttggcgatca atcttccaca
    25621 cattgcatac atttttttac tcctgatgtt aaagccagca atcgcattgc aaataacgcg
    25681 agggccattt taaacctagt gggcacctat tcagatcaaa accaaagtat aagccaaata
    25741 caatataacc agcaacaatg ccatttatta agggttgaaa tattgaattg acatggcgct
    25801 gtatgctaca ctcagcctta atcgccaaag gagatgcctg acatattatt ttcaaactgc
    25861 aaccaccccc acgagcacgc aaaccttatc accaactcag gagaaaacaa aatgcgtaag
    25921 tcagtatatc tggtcgtgac cgttgcaatc ttgttcgcct tgcttactac cgttacaggt
    25981 gccgcccagg ctgcccccgc tgcacaagcc aagtggactg tgatggttta catttcggga
    26041 gataacaatc tcgaagatta tgttgtaaaa gatttggaac tcgaactcgc acccaccggg
    26101 tccagcgcca acgtgcaagt ggttgcactt gcagaccgtg gtcccggcta tgacatcagc
    26161 cgcggagact ggcagaccac caagttattc catgtcacca ctggcatgat cgccgattct
    26221 gccagcgccg ttgcagattg gggtgaacgc aacatgggcg ataaacaaac gcttattgat
    26281 ttcgtttcat ggtcaaagac caattaccct gccgaccatt atgcgctcta tttctgggga
    26341 cacggctgga attggcaccc cggttacgtg atggaagatg tcacaaatgc cgatacactt
    26401 gacctcgacg aattgaaagc ggccctcccc tcactcggct tcattgacgt tgtcggctac
    26461 gacggatgca acatggcgtc cattgaggtt ttcaacgcct ggcatgggta tgcaaccgcc
    26521 gtctccagtt cacaggaata tgtcggctgg gatggcattg aatatgatgt cgtcctcgca
    26581 caactggcag ccaacccaaa catgaccgcc gaccaggtag cgatcgccac gagtcagagc
    26641 gcatccaacg acaagacctg gtctgctgtg gccgtggaca gccgcttcaa cgccttatac
    26701 acagccgtgg atcaatggtc agttgccttg aagaacggac ttgccgccaa ccgcaaaaaa
    26761 tatgaccgcg cttttggcgc cacccaggat ttctggcagg ctccaatgga caaagacttg
    26821 tacgacatgg cctacgagat caagcgcaac gtagccgata ccaacatcaa gaacaagagt
    26881 caggctgtga tggatgccat tgcaggtgtg gtgctgaacg agcaccacgt caacgggtat
    26941 ccggacgcac atggcatcac catctattac gtttccagcg ccgcaggcaa ggactcaaat
    27001 tacacctact atcgttccac ggtcgatttc gctttgacca ccggttggga tgagttcctc
    27061 gacgcatacg ccaaatgatc acttcggcaa gttaatacaa tcaggcctcc agaaatctgg
    27121 aggcctgaat tttttcgacc aacccaaaac tagcgcgatt gcgggctgtg attcggaagc
    27181 gcccttggct gacggggcaa cccacagtgc gggcagttca tcaatcggat ggaaaccgtg
    27241 taatcacgtg gacactcgca atacttcttt ttctccaccc gctttccctc atggtcaacc
    27301 cacttaccgg tatcatccaa ttcctggcgc ttatggcaca tctcacacca acggccacat
    27361 tcgtcactgg tataaaccca tttatgaaga ccaactttgc aaaatttcat agtaccttcc
    27421 tctggcggac cattcgccca acggtttacg ctaactgaca gttagacgga ctcggcgcgc
    27481 ccatcgcaaa ggggatgagc cgcagccaag gccgtagtat aaaccaattt ttacaggctg
    27541 ttcccatttc tttcattttt tcaaggtggg tcatcccttt acataggaca atttttgcga
    27601 gatcaaaccg tcctttaact gaaaaatatc cacaccacgt acgtgcccct tgttcccagt
    27661 cacatccacc caactgtatt tccaacgcat gacacaacgg attccatacc cgaagatctc
    27721 ttcgatctca atatgagcct gaggcgactc gcgaaagaaa tcctgccaga attgcgtaac
    27781 cgcctccctg cccgaataga ccgtcccatc cggggctggg ctggtattct caaaaacaca
    27841 atcatcgccc atcaaccgca tcatcccttc cacatcatga cggttgaagg cttcattgaa
    27901 ttcgatcaca acacgcacgg ctgattccac ttttgacatt cgcacagggc acatggcagg
    27961 acctcctaca tataaaacgc ataagcatta aaacgctgtt acgattatac cgacaattgc
    28021 ggcaacgatc cccagtccct gaatcttgtt gatcttatcc ttcaagaaaa taatggccag
    28081 ggtgatcgtc acaattgaca atgccgaagc aataggcgtg atcaaaattg catctccaat
    28141 ggtcaagccg taatttacga gcgccactgc accagcctca atcaccccca tgaggaaaat
    28201 gatggatttc gtgttcgcgg aaatgtgagt caggctgatc ccctgttttg tcaaaaacga
    28261 aaagatggac aggaatatga taatccctgc tttgatcaac agggttgtca ataaccatcc
    28321 gaccttttca gaaataatct cgctgacatt ccaaaaaacg ccaaagaaaa aagcacccaa
    28381 aatagcttcc ctgacccctg ccgaaagttg aaactttcgg tttccaattt cattccaatt
    28441 caaagacgcc acagtggcgc cacaaataat catcaagacg cccagggttt tcaaagcaga
    28501 gagtcgctgc cccatgaaaa cgaaagcaaa caacatggta aacacagccc ataggttcat
    28561 ggtcgctgcg atgatcgaaa cattacctgt ctcaaagcct ttaaagaaaa ataaatatcc
    28621 agcagaataa agaattgccg cgatcaggga tagcgcaacc accaaaatag ggatgtcaac
    28681 ggtcagcctg gcgaccacgg caaggatcaa aacggatatc aaacccgcca attgtgacca
    28741 aaacaaagac ctgaaagacc cgatctgttt ggcaaacaca ccgcctaaaa agtcatatat
    28801 cccccaccca aacataccgc cgatacccaa taaaatgctc aagatgattg tattcatgac
    28861 tcttttttac ccgtgaaatg tccaagccca tctgttgaga ttacgaatac actgcacata
    28921 cacttctaat acttactact tcagtccgtt gaattgccag tgatgacaac gcagcgattg
    28981 gttttcgagt cgtgcaacag atatgcaaaa cgccagagaa tgtaatgacc gggaaggcca
    29041 acatcacttc cataataata gagagtcgac gtttcaagcg gcacaagcag attgaggtgg
    29101 cgccagtatt ccgaaagaag tgcatcgatc aattgcggcc cggcagaacc tccacctaca
    29161 tatctgaaac attccgtaac gtactcaaca agctcatcag gctgggcgga ccgtattttt
    29221 gcttcaaagg gggtcgattt cggaaaaaac gtcatcatca actcagggat cggcaggtca
    29281 gatgcgggaa ctgtcgcagc atggacggaa agagttgtca catagttgag gccgcacaga
    29341 gttgcgtcaa tatgggagag caatgcccga aattcacgca aggcctcatc aggcaggggc
    29401 ttgatctgct gcaaaaccca cggctgcggt aatggtgaag ctttttgcgc tgtctgaaat
    29461 tgtgagggcc gcgtcatgcg cagatatgca aggcggtcat ccacaaagat ttttgcttct
    29521 gcaagttgaa cacccgtaaa ctgacgcagt tctcttaccg ccgcaacaag attcccgtca
    29581 agtagttgtt tatcaatgat cgcaagttgg tcaacagtta aattcaccga ggtttcgccc
    29641 tttgtatgat ttgatcgtat tattcccatt ttagtcattc agggtgtttt ccaacaccct
    29701 gaatgttcgt ggtcttcgtt acttcccgta tcgtttacgg atctctggcn nnnnnnnnnn
    29761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc accctgaatg ttcgtggtct tcgttacttc
    29881 ccgtatcgtt tacggatctc tggcaggaaa tactcgtcga aaaacttatc cacatcgtaa
    29941 tccggcgtcc agttccaatc tttgcgggcg cgggaatcat ccacatcctc tggccacgaa
    30001 tcgacgatcc cctgtcggcg cgggttgggt tcaaagttga ttttcgcatc gggaaaagcc
    30061 ttcaacgccc gctgacggaa ttcctctgcg gtgagtgcga acgctgcaat gttgtacacc
    30121 gtattggaca gtttctcgcg cggcacatcc atcagcatca acaaagactt gatcgcatca
    30181 ggcatggcca taaaagagat cttcgtatcg gcacgcacaa agcaggaata tggcttccct
    30241 tgcgccgccg catgcagcat ctcggggccg taatcgcttg tgccgccgct cggcaatgta
    30301 aaagcgctga tcaagcccgg gaagcggatt gcccgaaagt ccagcatgac cggtggtttt
    30361 tcatccaaat gtttctgacc gtagaatttt ccgtaataca tccccagttt ctcacaatac
    30421 aacttgttgc atccgtacat cgtgtgtgga taatcccact catcttcctt caccaacccc
    30481 gcttttcttt ttgcatccag cgttggcatg ccatacgccg caatcgaact tgggaacaag
    30541 aacttgacag ccttgccata cttttccgaa cgatacgccg ccagcatcaa caactgcatt
    30601 gtgccttcca cgttgatgcg gtgcgcctcc tccgttgcca cttcagcctt ggaagaaagc
    30661 gaagccgcca gatggaagat gatatcgaaa tcgtaatcgt aaaaggtctt gatcttgtaa
    30721 accagatccc cctgcacatg ttcggccgat aaatgtttga tcgaatccgg cagcggcata
    30781 aaatctgaag ttacgatccg atacccgcct cttgccgcca acccctgcac cagcgcctgc
    30841 ccaatttcac cagatgcacc ggttacaaga acttgcttat cgctcattgt caactcctta
    30901 ttatgctaga atgaagttat cttattttac gccacaatca aaatttccct gtcataaatt
    30961 ctccatgatg tttttacgtt ttcaaattcc cgccaaaaaa ttcctattga ccgccctcac
    31021 caccctgatg ctgaccagtt cctgcgccgt ggtggatgcg atgctcacca cccctacacc
    31081 gcaaatcccg acggaaaccc cgattcccac tgcgaccata gtctggttcc caccatcggc
    31141 cactccctcc gcccaaccat tttccaccca actccccacc cccgaaatgc gccccggcct
    31201 tggcagcatt gtcctcaccg acaacttctc aaacgaagaa gattggaaca cagccacatc
    31261 tgaccaggcc agcgccgcca ttgaaaacaa tcaactcatc ctcgcatcac agagcaaggt
    31321 atacatgctc agcctgcgcc gtgaccttgc cctcgacgat tactatgccg aaatcacggc
    31381 ccgtatcaac ctctgccgcg gtgacgatag ttatggcatc ctcgtccgcg ccaacgcagt
    31441 cgcttattac cgctttgcgc tgacttgcaa cagcaccgtc agtgcggaaa gagtcagtgt
    31501 aggcacacgc cagttattgc aatctcccct gcccagcgga gatgctcccc ccggagcccc
    31561 gggcgaagtg cgtatcggca tttgggctgt cggcacagaa atgcggctgt tcttgaacgg
    31621 acgctaccaa ttcagcatca gcgacacgaa ctaccccagc ggcaccatcg gtgttttcgt
    31681 caactccgca ggagatactg gcacggtcat ttcatttact gaactaacgg ttcgccaggt
    31741 caacgccgct ctccctgcag aatcatctcc gtaaatcacg cagatttaaa agaaaaatcc
    31801 gcaaaactct gcggatttat ttcttcacct tatctctggt cataaannnn nnnnnnnnnn
    31861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31921 nnnnnnnnnn nnnnnnnnnn nnnnnnaata tcattcaata actgtttcgc caacgcagac
    31981 atctcgcgat ctgcccgatt ttcggcgatc tgccgcaacg cagggatggc acgttcatcg
    32041 cgcaagttcg caagtgatgc cattgccttc ttttgcacaa cgcgatctgg atcatgcagc
    32101 atttcgatca atacaggtgc tacgcgcaca tcttgaattt ttcccagcgc ggtcaccgca
    32161 tgttcgcgta tggcggcttc ggggtgattc aaagactcga tcaaaatatc cacagcctgc
    32221 gacccaaatt ttgtcaacgc ctcggcggcg gcttcaccca cttcatggtg caggtcgtat
    32281 aaggccatgc ccaactcttc aatggcgcga atatccccca acttcgagag aatcatcgcc
    32341 gcaaacttgc ggaccgtccc ttccttatcg gcaagtgcat cgatcaatgg atacaccacc
    32401 gcttcgccca tattctcaat ggcggtcaac agcacagaaa cggcctgttc cttttcatac
    32461 caccagtagg aatcgcgcag cgcttccata agaaaaggga tcgcagcagg atgacgggta
    32521 tctcccaggg ctttcgcggc ggcctgtcgc acctcgatct tcaaatcgtc gagcaagaca
    32581 ttggtaatct catcaaaagt ggaagggtcg cggaattttg caacagcctt gcaggctgca
    32641 atgcggactt cgtcctcggg gtctttgagc aagggcaaca aatctgaaac ggttctttct
    32701 tcaccgatgg ctgcgagggc ccaaagggct tttgcccgca ccgtaaaata ttcgctcttc
    32761 aacgcatcca gcaggacggg tatgagcgcc ttatctttgc tgaagccaaa aacttcgatg
    32821 atccgtccgc ggatgacggg atgcgcggtg gaaagtacct tgatcaattg aggttgggca
    32881 ggagatattc gggcgagaag atgctgatag agcagaagca ggttcaagtc ttgggtttga
    32941 agcgcttcca tcagggccgg cacggcatcg gcgccgagtt tcaagaggct ttgtgaggcg
    33001 gcatcacgtt tcgtcgaatc cgcaagctgc gaaatcaact tccttgcttc tccctgtttc
    33061 gaacctgtga gccagtccat gtttttattc taatctaaaa tttatcagtg aagaaaagtt
    33121 caaaggaagt atctttcaaa aactctcagg ccagaaatcg gccaattttt ctctgatata
    33181 atccccgatt cgatttgcgg gaatcataac gccagagggc ttttggtgtt cttttcctaa
    33241 gcatcacact aattttcagt gcgctttggc gcacgataaa ccttatcgga gttcccaact
    33301 aatgaagaaa gaacaacagt tagacctcta ttaccagatg gtactgatcc gtcgtgcaga
    33361 agaacgcggc gcggagttgt accagcaggg caaaatcggc ggcttcatgc atctttatat
    33421 cggtcaggaa gccgtttcga ctggtttgat cgcggcacgt gaaccacgtg accgtgtcat
    33481 cactgcctac cgtgaccatg gcgtggccat caattgcggc atttccacca acgaagtaat
    33541 ggcggaactg ctcggcaagg ctacgggcat gtcgaagggc aagggcggtt caatgcacat
    33601 ggcaagcacc gagaagaaca tgtggggcgg acacgccatc gtcggcggcc acctccctgt
    33661 tgcggcagga ctcgcccttg gcgaccagta cgccaagaac gacaacatca ccatctgcat
    33721 gtttggtgat ggcgccacca acatcggcta cttccatgaa gccttgaacc tcgccaagac
    33781 atggggattg cgggtgttgt gggtttgtga aaacaaccaa tacggcatgg ggaccgcggt
    33841 tgagcgcgcc tcggctgtga ccgagatccg ccaaaaggct gaaggctatg gcatgaaaaa
    33901 cggccaggtg gacggcatgg atgtgatgaa ggtttacgaa gccgcaaaag aagccttgga
    33961 ctttatccgc aaagagggcc agccctacct gcttgaaatt gacacctacc gcttccgcgg
    34021 tcactccatg ggcgaccccg aacgctaccg caaagctgaa gaagtgaagc agtggcagga
    34081 aaatgacccc atcggcatct acaacaagta tctgcttgat aaaaagatcg ccacccgcaa
    34141 gcaattggat gaaatcgaat cgcgcgcgga cgaagaagtg aacaaggctg ttgagtttgc
    34201 agaagccagc cccgaaccca caatggaaga cctgtacgcg gatatttacg cggataacta
    34261 aaaccactaa gacacaaagt cacggaggca caaagaagta aaaatcttgg tgtcttgggg
    34321 tcttcgtggc ttcgtggtta aggaataaca caatggcaaa aattacaatg cgcgaggcga
    34381 tctcgcaagc tttaatggaa gaaatggacc gcgaccccgc cgtctttatc atgggcgagg
    34441 aagtcggtgt ttggggtggc acttatgccg tcaccaaagg tttttttgac aagtacggtg
    34501 cagaccgcat caaagacacc cccattgcag aaaacgccat cctcggtgcg gccatcggcg
    34561 cggcgatggt tggtcagcgc ccgatcgctg agttgatgac catcaacttc gccttctcgg
    34621 caatggacta catcgtcaat caagcggcca aactgcgcta catgttcggt ggacagttca
    34681 tgttgccgct cgtcatccgt gccgtcggcg gcggcggacg ccaacttggc gctacccact
    34741 cgcagactcc cgacgccatc ttcgcgcact tccccggtct gaaagttgtc agccccggaa
    34801 cgccagaaga tgccaagggt ttgctcaaat ccgctattcg gtcgaatgat ccgattctct
    34861 tcatcgaaca cgccaccatg taccaggtcc gcggcgaagt tcccgaaggt gaatacacca
    34921 tcccgatcgg caaatccaaa attcagcgcc ccggcaaaga ccttaccatc gtcacgtact
    34981 gcaaaggtct tgagctttcc atgaaagccg ccgacgatct cagcaagcaa ggcatcgaag
    35041 ccgagatcgt tgacctgcgc accctgcgcc cgctcgatat ggaacccgtg ctcgaatcct
    35101 tcaagaaaac caaccgcgcc atcgtcgttg aagaaggctg gaagtcttac ggcgtcggca
    35161 gcgaaatcgt cagccgcatc tacgaagaag ccttcgatta tgtagacgca ccgatcatcc
    35221 gcgtggcaca aaaagaagtg cccctgcctt acaaccgcac cctcgaacaa gccgcactcc
    35281 cgcaagttca cgacatcgtc gccgcggcaa aggaggtctt gaaataatgg ctgaaacaat
    35341 caacatgccc aaacttggct tcgacatggc ggaaggtctt ctcgtccgct gggtgaagca
    35401 agttggcgaa aatatcaata agggcgatgt cctcgccgag atcgaaaccg acaaagccac
    35461 cgtcgaagtg gaatcttccg ccagcggtgt tgtgctctca cacatcgtcg atcaaggcag
    35521 catggtgccc gtcaatgcac cgattgccgt cgttggcgct gccggtgaaa aagtggatgc
    35581 gcctgcggcg gctccaaaag cagaagccaa agcggaagaa aaacctgcgg ctcagactca
    35641 gccgacagct gcttccgctc cctctgggca ggcctctgcc cccgctgaca atggtcccgt
    35701 gaaagcatct ccgct
//
LOCUS       DAOQLC010000031        35058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_375_length_35058_cov_24.573322, whole genome shotgun sequence.
ACCESSION   DAOQLC010000031 DAOQLC010000000
VERSION     DAOQLC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 35058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_35058_cov_24.573322"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..376
                     /gene="rpsJ"
                     /locus_tag="PL000_07475"
     CDS             68..376
                     /gene="rpsJ"
                     /locus_tag="PL000_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNC88775.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERTGAQVVGPVPLPS
                     KIERFTIRRSAFIDKDSHEHFEIRTHNRLIDVLDPDSKTIDMLMRLNLPAGVDIEIKI
                     "
     gene            561..1193
                     /gene="rplC"
                     /locus_tag="PL000_07480"
     CDS             561..1193
                     /gene="rplC"
                     /locus_tag="PL000_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNC88776.1"
                     /translation="MLKGLIGKKIGMTQIFDEQGVAYTVTLIEAGPCYVTQVRTPEKE
                     GYSSVQLGFGEVNPKRLTGGQLGHFKANEISPVRFLREFRAKDHGLKVGDKVTVVDAF
                     AVGERVDVVGVSKGKGFAGGVKRYHFHGMNATHGTSDRNRAPGSRGSGTTPGRVYKGA
                     RGAGHMGVDRVTVQNIKVVMVDAERNLLAINGAVPGGKGSLVMIKESRKQ"
     gene            1222..1851
                     /gene="rplD"
                     /locus_tag="PL000_07485"
     CDS             1222..1851
                     /gene="rplD"
                     /locus_tag="PL000_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNC88777.1"
                     /translation="MKVDVFDLKGQKVREMELPANVFEAPVNVDLMHQAYVRQMANAR
                     LGTHETKVRGEVRGGGRKPFKQKGTGRARQGSTNAAQWVGGGRIHTPHPRSYEQRMPR
                     KMRLAALRSVLSAKAADAGIVVVDDLDIKEPKTRVMAEALGNLVGSSTALVLMPVKDQ
                     SYDNVMRSASNIESAKILLASYLNVRDVLNFDKVVLPLKTIDALVAHLG"
     gene            1861..2166
                     /gene="rplW"
                     /locus_tag="PL000_07490"
     CDS             1861..2166
                     /gene="rplW"
                     /locus_tag="PL000_07490"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNC88778.1"
                     /translation="MTTIHDVLVRPLVTEKSSFQSGKLGQYVFVVADKATRTMVKDAV
                     ETLFDVTVARVNILNAPAKRGRRTRSRRLLVRRPSFKKAIVTLAEGSKSLEIFEGVQ"
     gene            2166..3002
                     /gene="rplB"
                     /locus_tag="PL000_07495"
     CDS             2166..3002
                     /gene="rplB"
                     /locus_tag="PL000_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNC88779.1"
                     /translation="MAVKKYKPVTPGMRDMTGYTFEEITKSTPERSLLVKKTARGGRN
                     AQGRVTVRHRGGGARRYIRIVDFKREKHGIPAKVAAVEYDPNRTARLALLFYADGEKR
                     YIISPLDLKVGDAVMSGPNAEIRPGNALPISNIPVGTLIHNIEVKEGKGAQLVRSAGG
                     AAQLLAKEGDFAQIRMPSGEVRLIHQVCYATIGQVGNLDHGNVKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPVGLPGPKSPWGKPTLGKKTRRNKKTDQYIVRRRSKT
                     NR"
     gene            3025..3297
                     /gene="rpsS"
                     /locus_tag="PL000_07500"
     CDS             3025..3297
                     /gene="rpsS"
                     /locus_tag="PL000_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNC88780.1"
                     /translation="MSRSLKKGPYIEPKLLKRIEAMNQKNEKKVIRTWSRPSVIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRTFRGHQTKEAAA"
     gene            3309..3656
                     /gene="rplV"
                     /locus_tag="PL000_07505"
     CDS             3309..3656
                     /gene="rplV"
                     /locus_tag="PL000_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNC88781.1"
                     /translation="MSDIHAKLSHLSLSAQKVRIVIDMVRGKSANEALEILRFVNKRA
                     ALPVQKLVASAVANAEENFGVSRDDLYVAKITADEAPTRKWRRFGARGRFKPILRRSS
                     HVTVVLRERGREA"
     gene            3666..4415
                     /gene="rpsC"
                     /locus_tag="PL000_07510"
     CDS             3666..4415
                     /gene="rpsC"
                     /locus_tag="PL000_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNC88782.1"
                     /translation="MGRKVHPVGFRLGINQPWGGRWFAEGSTYRQQLHQDLAIRSLLL
                     GKFSKGGAAASEVVRGLRKQMPDAVKDGKAGIARVDVERTPGKVRVSIHTAKPGILIG
                     RKGEGVKKIRQALETLAGKKVELDIKEISSPDTDAMLVANNIADQLERRIAYRRAMKR
                     AIQQAIKQGAEGIKILVGGRLGGAEMSRDVWLREGRVPLQTLRANLDFATTEALTTYG
                     QIGIKVWIYKGESAPEVEEKAEATEGVYVSE"
     gene            4447..4866
                     /gene="rplP"
                     /locus_tag="PL000_07515"
     CDS             4447..4866
                     /gene="rplP"
                     /locus_tag="PL000_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNC88783.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKALRGAEVSFGEYGLQALEPGWVTA
                     RQIEAARRSIVRGMKRRGKVWIRIFPSKPFTHKPPETRMGSGKGNVEYYVAVVKPGRV
                     MFEVGGLPGDIAVAALKSAMYKFSIKTKVVGRHAEGE"
     gene            4869..5084
                     /gene="rpmC"
                     /locus_tag="PL000_07520"
     CDS             4869..5084
                     /gene="rpmC"
                     /locus_tag="PL000_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNC88784.1"
                     /translation="MKSMKIEDIRKMAAEEIRKKLTDTRDELMKLRFQQVSGQLTDTS
                     SLPALRRNIARMETVLRELENAVVEGA"
     gene            5084..5356
                     /gene="rpsQ"
                     /locus_tag="PL000_07525"
     CDS             5084..5356
                     /gene="rpsQ"
                     /locus_tag="PL000_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNC88785.1"
                     /translation="MNNRRRMTGVVTSNKMTKTVVVEITRKFRHPLYKKVVYSSARVK
                     AHDEIGCQIGDEVQIVETRPLSRDKRWAVETIVKRETRTADQGVGE"
     gene            5360..5731
                     /gene="rplN"
                     /locus_tag="PL000_07530"
     CDS             5360..5731
                     /gene="rplN"
                     /locus_tag="PL000_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNC88786.1"
                     /translation="MIQQESRLKIADNTGAREILVIQVLGGSRRKYGAIGDIVVATVK
                     AAAPQGSVKKSEVVKAVIVRCKKEWRREDGSYIRFDDNAAVILDADGENPRGTRIFGP
                     VARELRDKGYMKIVSLAPEVL"
     gene            5741..6070
                     /gene="rplX"
                     /locus_tag="PL000_07535"
     CDS             5741..6070
                     /gene="rplX"
                     /locus_tag="PL000_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNC88787.1"
                     /translation="MKVKIRKGDTVEVISGRLEDKGKRGEVINVILSDRRVVVQGVNI
                     RTKHQKQVQAGNRQMNPGRIQFEGPVDISNVMLVCPKCSEATRVGIQRDDDGAHRVCK
                     NCDALID"
     gene            6088..6642
                     /gene="rplE"
                     /locus_tag="PL000_07540"
     CDS             6088..6642
                     /gene="rplE"
                     /locus_tag="PL000_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNC88788.1"
                     /translation="MNKMQELYNNEVAPALFKTFGFKNVMQVPRIEKVVLNIGLGEAM
                     DNPKALEAATADLMQITAQKPVQTKARKSIANFKLREGRLIGAKVTLRGPRMWAFIDR
                     LFNIALPRVRDFRGVSANAFDGRGNYTLGLKDQLVFPEIEYDKIDKLRGMEITIVTSA
                     QNDDHARALLRLLGMPFSSNKKDA"
     gene            6646..6828
                     /locus_tag="PL000_07545"
     CDS             6646..6828
                     /locus_tag="PL000_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNC88789.1"
                     /translation="MAKKCMQYRELRRRHAVQVRNRCQRCGRPRGFIRRFGLCRICFR
                     ELALTGKIPGVVKASW"
     gene            6848..7261
                     /gene="rpsH"
                     /locus_tag="PL000_07550"
     CDS             6848..7261
                     /gene="rpsH"
                     /locus_tag="PL000_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNC88790.1"
                     /translation="MSFTDPIADMLTRIRNAVLAGHAQVAMPNSKIKVEIAKILKDEG
                     FLEGFEVVEGEREAQKVLRVKLKYVGERRQRKAVLSGLERVSKPGRRIYTKKTDIPWV
                     LSGIGVAIISTPKGVMTGARARQLGVGGEIICKVW"
     gene            7272..7820
                     /gene="rplF"
                     /locus_tag="PL000_07555"
     CDS             7272..7820
                     /gene="rplF"
                     /locus_tag="PL000_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNC88791.1"
                     /translation="MSRIGRLPIDIPSGVQVSVNGLDVHVKGPKGEMKRSFSRMVGIK
                     MENNQLNITRNSEEPAERAMHGTARAVIANMIHGVSKGFEVVLQVEGVGYRAEMEGKN
                     LALFVGYSHPIKMEPPVGVTFETDAKTRQIKVSGYDKELIGQVAANIRKVRPPEPYHG
                     KGLRYLGERVRRKAGKAGKGAK"
     gene            7824..8189
                     /gene="rplR"
                     /locus_tag="PL000_07560"
     CDS             7824..8189
                     /gene="rplR"
                     /locus_tag="PL000_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNC88792.1"
                     /translation="MANKSRSLARERRHGRVRKHVYGTSDRPRLNVFKSLSGIYAQII
                     DDNEGNTLVSASTVDKELREKMKGLKKTEQAKAIGKAVAERAKSKGISAIVFDRGGFR
                     YVGRIKALADGAREGGLQF"
     gene            8235..8783
                     /gene="rpsE"
                     /locus_tag="PL000_07565"
     CDS             8235..8783
                     /gene="rpsE"
                     /locus_tag="PL000_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNC88793.1"
                     /translation="MAEKQFTEKQQFETQDAFEERVIEIARVAKVVKGGRRFRFRVTV
                     VVGDKNGTIGMGIGKANAVPDAMRKASERARKDLRVVNLSNTTIPHESIGKVAGAKVF
                     LKPASAGTGVIAAGGVRAVLELAGVRDILTKSMGSANVLNVVMATFGALEGLRSPANE
                     AARRGKRAEELVPFWERRKQHA"
     gene            8776..8973
                     /gene="rpmD"
                     /locus_tag="PL000_07570"
     CDS             8776..8973
                     /gene="rpmD"
                     /locus_tag="PL000_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNC88794.1"
                     /translation="MPKQTTSGKTLRVTWVKSDIGFPKDQKATLKALGLRRLHQTVEH
                     KDTPALRGMLNKVVHLLKIEE"
     gene            8982..9437
                     /gene="rplO"
                     /locus_tag="PL000_07575"
     CDS             8982..9437
                     /gene="rplO"
                     /locus_tag="PL000_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNC88795.1"
                     /translation="MKLHDLVPNAGSKKNRKRVGRGISAGQGKTAGRGTKGAGARSGE
                     GGHAYRQGGNLPFFRRLPFIRGEGFTPPNQVFYNEVNLDQLAQAFQANADVTPESLEQ
                     AHLLRDSRNPIVVLGRGEMTVALKVRVHRVSAGAKAKIEKAGGSVELIA"
     gene            9518..10846
                     /gene="secY"
                     /locus_tag="PL000_07580"
     CDS             9518..10846
                     /gene="secY"
                     /locus_tag="PL000_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNC88796.1"
                     /translation="MIVTFAILAIYRLAANVPAPGVNFDVLAQFRASSAGSGGFLGFL
                     DLLSGGTVSNFSLLSMGVYPYITAQIILQLLIPIIPALQRRMEEDPREARRWQEKWTY
                     YLAVPMAALSAIGQINIFNSLSAQAIGQTILPFGLFSSDLWLPSLTVLLSMTAGTMFA
                     IWLGELISEYGIRGQGLSLVIFAGIVARMPANLVSLLSDESTRWFMLIFTIIIIILTV
                     FAIVYVQQGRRNVPVMYPGRRMGNRMSMPVKGTLPLMVNLSGMIPLIFASAILQFPAI
                     IASYFTSSENAGVAKFFTDVQNFFGATSTTTGNWGYWLLYFFMVVVFTFFYTAVLFDQ
                     QNYGENLKKVGATVPGVHTGAPTQKYLSTVQRRITLVGAVFLGVVAIMPFLLGLLLRA
                     LSLNAASSQAGLFLVSSSGLLIVVGVVRDTFQNIDAELKLHGYKDSLLVR"
     gene            10856..11506
                     /locus_tag="PL000_07585"
     CDS             10856..11506
                     /locus_tag="PL000_07585"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC88797.1"
                     /translation="MTTFIVLLGPPGVGKGTQAKILADATGLAHISSGDLFRENLKNQ
                     TDLGKLAKSYMDKGELVPDDVTINMIRDRLKRDDCKSGAILDGFPRTPAQADALEKML
                     SEFNGHVDSVPYITAAEDILIERTGGRFTCRASGHIYHEKFNPPSKVGICDVDGSELY
                     QRDDDKKETVTKRIRVYLDQTMPLVEYYRTRGRLVEIDGDQPVEQVTQRLLAALKK"
     gene            11564..12334
                     /gene="map"
                     /locus_tag="PL000_07590"
     CDS             11564..12334
                     /gene="map"
                     /locus_tag="PL000_07590"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNC88798.1"
                     /translation="MSWERQIKVKSPAELKIMREAGHINASVLAAVRELLQPGVTTAD
                     LNAAAEEVLKSHGCVSPFKNYGHPPFPASITVSINDEMVHGIPHPKRKLKDGDIVSID
                     CGTTYNGFVADSAFTAGVGEISPQATKLLEITENALYAGIDKMRKGNRTGDVSAAIQK
                     YVEDNGFHVTREYTGHGVGRKMHEGPQVPNIGIPGTGLQLRPGLTIALEPMVLVGTHE
                     TRVLPDQWTVVSADGSLTAHFEHTVAVTEGEPLILTVL"
     gene            12435..12548
                     /gene="rpmJ"
                     /locus_tag="PL000_07595"
     CDS             12435..12548
                     /gene="rpmJ"
                     /locus_tag="PL000_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNC88799.1"
                     /translation="MKVSPSIRKRCAKCRIIRRKGIVYIICENPKHKQRQG"
     gene            12560..12946
                     /gene="rpsM"
                     /locus_tag="PL000_07600"
     CDS             12560..12946
                     /gene="rpsM"
                     /locus_tag="PL000_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNC88800.1"
                     /translation="MARIEGVDLPRNKRVEVGLTYLYGIGPTRAQAILANTKVNPDTR
                     IKDLSEADVAAIRDYISKHYKVEGDLRREVQMSIKRLIEIGSYRGLRHRRNLPVRGQR
                     TRTNARIRKGTKKTVAGRGRRKGAKK"
     gene            12972..13376
                     /gene="rpsK"
                     /locus_tag="PL000_07605"
     CDS             12972..13376
                     /gene="rpsK"
                     /locus_tag="PL000_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNC88801.1"
                     /translation="MAQSVRSTRRPSGAKKGKRTLSYGQVHIFASFNNTIVTVTDTEG
                     NTVSWGSAGSAGFKGSRKSTPFAARLAAEQALKAAQQLGLQDVDLFVKGPGPGRENAI
                     RAVQSMGLKVRSISDITPVPHNGCRPPKKRRV"
     gene            13397..14062
                     /gene="rpsD"
                     /locus_tag="PL000_07610"
     CDS             13397..14062
                     /gene="rpsD"
                     /locus_tag="PL000_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNC88802.1"
                     /translation="MAKTLSPVCKLCRREGEKLFLKGERCFTPKCSFEKRDFAPGQHG
                     RTAGRGGGKGKGMGTGRASDFARQLRAKQKARRIYGVMERQFRRYYDTAKSGQSLTGL
                     TLLQILESRLDNVVFRLGFASSRSQARLLVAHGHFKVNGRRTDVPSMLLSEGDVVSVR
                     EGSSKLTYFKDLSAYTEKRTAPSWLNRDIKAMAGTLARMPERAEIDGSLDEQLIVEYY
                     SRR"
     gene            14160..15110
                     /locus_tag="PL000_07615"
     CDS             14160..15110
                     /locus_tag="PL000_07615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNC88803.1"
                     /translation="MVMPKIEREAEARNYGKFTIGPLEGGYGVTLGNALRRVLLSSLE
                     GTAITSIRFADVLHEFSDVPGVREDVIQIMLQVKQIRIKMDGVDSARMHLEVRGEGTV
                     TAADIVTPAEVEIVNPDTYLFTVDGAKTKHDIEFVVERGRGYSPANERAGHMPIGELP
                     VDAIYSPVKRVNWEVSSARVGQSTNYDKLILEIWTDGTISPERALSSSARILIDHLRY
                     IAGINEEMLAVAVERESSGSRLSSELAETPVEALDLSVRVFNSLKRTGITNVGDVLDL
                     LEKGETAVMSIRNFGEKSLDELRQKMQEKGFMKDDTSSES"
     gene            15114..15491
                     /gene="rplQ"
                     /locus_tag="PL000_07620"
     CDS             15114..15491
                     /gene="rplQ"
                     /locus_tag="PL000_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNC88804.1"
                     /translation="MRHSVAGYILGRTKSSRIALRRNLIKQLFTHERIKTTEAKAAAV
                     RGDAERLITLARNSANGTDAEKLSARRLAISKLGDNQLIKRLFDEIAPRFATRPGGYT
                     RVTKLGPRLGDAAEMVVLELVEE"
     gene            15542..16288
                     /gene="truA"
                     /locus_tag="PL000_07625"
     CDS             15542..16288
                     /gene="truA"
                     /locus_tag="PL000_07625"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNC88805.1"
                     /translation="MARYKVILAYDGTGFTGSQRQANSRTVQGEFEKALRRLGWSDKS
                     IVMAGRTDTGVHATGQVAAFDFDWAHTEEKLLRALNSDLPSDLVIKSLITAPADFHPR
                     FDAASREYRYRLFCGTIRDPLREKYFWRVWPALDEDALKQAAAIFLGTHDFATMGSPT
                     TPKGTTVRTVTKAEWKRMPDDEWQFEVRADAFLYRMVRRLVFVQVSVAQGKAPLEKVR
                     KALDEKGKLPSGLAPAQGLTLVEVTYGSHD"
     gene            16311..16754
                     /gene="rplM"
                     /locus_tag="PL000_07630"
     CDS             16311..16754
                     /gene="rplM"
                     /locus_tag="PL000_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNC88806.1"
                     /translation="MQQKTFYPKAAEIQRDWVVIDAEGQTLGRLAARVAHVLLGKHKP
                     TFTPGVEMGDFVVVVNTERVSVTGTKTSSKLESKIYYSHSGYPGGLKSISLRDQLATH
                     PDRALRDAVWGMLPHNRMGRAVLKRLKIYAGPNHPHAVQTAKAAK"
     gene            16770..17162
                     /gene="rpsI"
                     /locus_tag="PL000_07635"
     CDS             16770..17162
                     /gene="rpsI"
                     /locus_tag="PL000_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNC88807.1"
                     /translation="MAQYYEGIGRRKASTARVRVSTGSGKFTVNEKEGAAFFTRMGDV
                     EDIMRPFSAVGQEAVKYDVSAVVNGGGTSGQTESVRLGIARALQLINAEWTAALRKKG
                     LLTRDARVKERKKPGLKKARKAPTYTKR"
     gene            17285..18388
                     /gene="mazG"
                     /locus_tag="PL000_07640"
     CDS             17285..18388
                     /gene="mazG"
                     /locus_tag="PL000_07640"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNC88808.1"
                     /translation="MDFSLVSQTFETLRIAPPSKLTLLEAHTLSPEFAHVPPFPPDIP
                     ALLTGIASRELALQVRTVLRTVYPQEHEVFVVEAGVAREHSLSELGTGEFSEQTCLYL
                     PSLAAGTSFESFAEIVAHLRAPDGCPWDKEQTHQTLRKHLLEEAYETLSAMDENNSDE
                     MREEFGDLLLQIVLNSQIANEAGEFTMNGVIKGIYDKIVRRHPHVFGDVKLDGVDGVL
                     QNWEKLKEQERKEKGEDGGKKKGLLDGVSAALPALTQAQEYQDRAARVGFDWPEIEGV
                     LDKIREEIEEIKKAQNLDEVTAELGDLFFVLVNLARWRKVDAESALRGTNQKFKKRFA
                     YVEQGAKQQGRNLSDMTLEEMDILWNEAKRVGI"
     gene            18482..19465
                     /locus_tag="PL000_07645"
     CDS             18482..19465
                     /locus_tag="PL000_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88809.1"
                     /translation="MESQLGTPSIPQKPKWDLPDVWAKSGHCPACGATNLKITHLPDY
                     PDYLSCAHCQVSFEVENGGRHIRLKYIPDQFEYADAMLRGRWVAAADLPAILRKPQPV
                     VKEADEPQLPKQPLSDAEIWKRAVGMYKLGNKPRMIQLTLMQSGVPQAQADAILEKLK
                     RLAEEDTRKMNQKFWILAGVALLLIFSLAGGWLYGSGNLAVLMGDATPVPTQSGPSAV
                     DLLLDLVPDEAKPAIIDLPKTSVDVGRGPGQASCPPTSGMAAELFGGDAAMWQQAGAF
                     AAWQMIDPINSATITVPTDMTAAYVDNRTRQFLSVHGPATIYNVNFLVISC"
     gene            complement(19473..20009)
                     /locus_tag="PL000_07650"
     CDS             complement(19473..20009)
                     /locus_tag="PL000_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88810.1"
                     /translation="MPPSETDFTIRHINPADLQVFRELRLEALKNHPSAFGSDYEETL
                     LQPQSYWEKRLTFQADEQALFFSEHHGQPIGMAGIVRNNSKKNHHSATIISVYVKPEW
                     RGRRIAEMLIHACLGWAAEHDVVIVKLAVVTDNLSAIHTYERCGFKIYGTEPKALRIN
                     GIYYNEHLMAVEIPHPSK"
     gene            complement(20036..20671)
                     /locus_tag="PL000_07655"
     CDS             complement(20036..20671)
                     /locus_tag="PL000_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88811.1"
                     /translation="MKKTALLFPNLCLILLLMAACSPKTTLPTLDPNIQIQQAVAETL
                     AAIPTATPYTPPTPFPSATPISLAGVFCEYQFCIGHPNNMAFYDHMAALNQLTPSAYN
                     NGFLAAYQIPTMVINFIWLQAPGTSDPQFLLETLLDDQADTKNGTFDVKLIRGMNVMY
                     VPITTTISEVPFGAIAAWTCGDRVFAWKVYTPQAETAGPLFEEALARFTCN"
     gene            complement(20737..22500)
                     /gene="nirK"
                     /locus_tag="PL000_07660"
     CDS             complement(20737..22500)
                     /gene="nirK"
                     /locus_tag="PL000_07660"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HNC88812.1"
                     /translation="MKTFLKFLVAAIFLGILVSACTGTSFQPVDKEYVLTTDLREGRL
                     VFLGVSDEINGLENPTLSARPGSTITVTLINGGEGQHNLTFPEVQASTAVLTEKGEEA
                     SVTFTVPNTHGEMEYYDDVANHAELGMRGKLMVSETDQPMPVAAEQSNGDPQVLAAFQ
                     KGACGSCHAIDGIPNAVGVIAPNLSDINTAAEEHLKSDAYTGTATTTEEYIRESIADP
                     NLFVAPTCPTGACAPNVMPATLKDALTEEEINSIVNYLSGLPEGAYSTSTEAAAPQAP
                     TTGADVVRDPTDLPAPLAVREPQTVRVDLETIEVEGQLADGTTFTYWTFNGAVPGPFI
                     RVRVGDTIEVHLKNSGSSTMNHSVDFHAVTGPGGGAAVTQTEPGKETTFTAKAINPGL
                     YVYHCATPMVANHIANGMYGLILVEPEEGLPPVDREFYVMQGDIYTTGAYGDHGMQTT
                     DVTKLLNEDPEYVVFNGAVGALTDQKPLKANVGETIRIFFGVGGPNLTSSFHVIGEIF
                     DRVFDQASITSAPLTDVQTTLVPPGGATMVEFKLEVPGRFILVDHALSRLQRGLAGFL
                     IVEGDPNPEIFDGTPAPGSGH"
     gene            complement(22514..22747)
                     /locus_tag="PL000_07665"
     CDS             complement(22514..22747)
                     /locus_tag="PL000_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88813.1"
                     /translation="MRYSIVTMNESFEISEASVEDILRSIPGAARLFLNWRTGCVGCG
                     FARFCTWKDVVKTYQLDGQKALEELKQANVQNP"
     gene            complement(22803..23501)
                     /locus_tag="PL000_07670"
     CDS             complement(22803..23501)
                     /locus_tag="PL000_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC88814.1"
                     /translation="MNLSPKDLKQVIVFQNATDDDLRLILQNSVERSMEESGFFFFHG
                     DPAHYLYILTSGQVKLMQNSVNGQQVNLRIIYPWQMFGALGAVRAEATYPASAQALEN
                     STALAIKSEFLHEMIQTRPQLSFDLMTLMTTYIQEMQARYRELATERVEQRVANALVR
                     LAGQAGIRSEKDAAIELSFSRQDVAEMTGTTLYTVSRLFSDWERQGIITTGRERIRII
                     KPHDLVRIADGLEK"
     gene            23590..24636
                     /locus_tag="PL000_07675"
     CDS             23590..24636
                     /locus_tag="PL000_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HNC88815.1"
                     /translation="MKKIFVVLGVVFMMAVLAIWKYQQVELYQFHGSVFREPKLAAEI
                     VLRSASGPVRLADYRGKITLLYFGYTSCPDVCPTTLANLKLALENLAPADASQVQVIF
                     VSVDPDRDTPERLGKYVQLFNSGFVGATGTRQEIDLMTDAFDVYYKINNSESKENYSV
                     DHSGFVTVIDRHGYAVMSWPHGTAPRDVSEDLQYLIAHDMPVSAQILAGPTYTPVVCA
                     VTLAPAHIQGGEWLYQHHCAQCHGADLGGNASWQVELADGTHLPPPLDGNGKAWQYSE
                     TELIEIIKNGRNLDKPLFMPAFKTTLADWEISFILTYLKSHWSINQLNYQHGFMTLTP
                     QATPSTLPTNTALP"
     gene            24790..25062
                     /locus_tag="PL000_07680"
     CDS             24790..25062
                     /locus_tag="PL000_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNC88816.1"
                     /translation="MMENDNTLRRLKTVEGHLRGVIRMVEEDAYCIDVIRQIQAIEAA
                     LNKVSSKILEDHLNSCVITAIQGSDKKERERVLKEITEVFEMSTKV"
     gene            25154..25372
                     /locus_tag="PL000_07685"
     CDS             25154..25372
                     /locus_tag="PL000_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNC88817.1"
                     /translation="MTTVTYTVPAISCGHCTRTIETEVSDLQGVQTVKAEIESKKVTI
                     TFDAPADETKIKALLAEINYPAEGLLTL"
     gene            25430..27901
                     /locus_tag="PL000_07690"
     CDS             25430..27901
                     /locus_tag="PL000_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HNC88818.1"
                     /translation="MTDTKQLTLPITGMTCANCVATVERNLKKLDGVQSAVVNLSSER
                     ATVDFDAAKLGLTDVIARVNRAGYGVATGEADLIIKRLSDGNDAARLEKALVKLEGVL
                     EAQVTFANEKARVKYIPTIITQAELRRAVSSAGFEALELGGEAEDAEALAREHEINEQ
                     RRLLIIGLVFTVPLFLLSMSKDFGLLPDFFYTGTTMDGMRDAQPWFGWLMLVLALPVQ
                     FYVGWQYYVGAFKALRNRSANMDVLIVMGSSAAFFYSLPITFGWLMGHVYYETAAVII
                     TLIKLGKFLEARAKGRTSEAIKKLMGLRAKTARVIRDGVEAEVSIDDVRVGDMVLVKP
                     GEKIPVDGAVVEGRSAVDESMLTGESLPVEKKPGDAVIGATLNKLGMLKFEATKVGRE
                     TALAQIIKLVEDAQGSKAPIQKMADQVSAVFVPIVIGIAFLTFLAWYFFGPALPINSD
                     IDNFTRALIYMVAVLVIACPCAMGLATPTAIMVGTGKGAEAGILFRNSEALERAGRVS
                     VVVLDKTGTITKGQPAVTDIVTNNESPMTGDELLRLAASVEKGSEHPLGESIWAEATT
                     RGLVLVEPAGFKAEAGHGVQAEVEGRSVMVGNRRMFEARGIQLGNLESEVNRLQSEAK
                     TAMLVAVDGLAVGIIAVADTIKDTSKDAITNLHEMGLKVAMITGDNQKTADAIAKQVG
                     IDMVLAEVLPEGKSAEVKKLQSDQSPTTHVVAMVGDGVNDAPALAQADVGLAIGTGTD
                     VAMAAAPVTLMSGDLRGVSRAILLSRKTLSTIKQNLFWAFIYNVILIPAAALGYLNPM
                     LAAGAMAFSSVFVVTNSLRLRSVKI"
     gene            27953..28456
                     /locus_tag="PL000_07695"
     CDS             27953..28456
                     /locus_tag="PL000_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88819.1"
                     /translation="MKKLTLLLLAVLFLTACAPKAAEMPKAELPAGKAWADGKEIYFI
                     HTEASDAGVAEKLTNMMKSPVVLVPSLANVPAESLANVYVFTNGLAGTGPFGFQADVF
                     DNPPGTDGYTPLRSLHLITWTDETKARELTSAADVLAAEAAGELTIEQPGVVINMPFV
                     VWDGGKR"
     gene            28492..29664
                     /locus_tag="PL000_07700"
     CDS             28492..29664
                     /locus_tag="PL000_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC88820.1"
                     /translation="MKKNKNKPFDPVIIITVGVTFLAVAFMLLVKPQSLAGDSSAMPQ
                     LMVAFVTGLTTGGLSCLAVQGGLLASSLAHQIEQDYVAGQAAKKAASPRTNSALPILL
                     FLISKIAAYTLLGALLGILGSYLTLSPLTRAMLMILIGIFMIGNALRMFNVHPIFRYF
                     SIEPPSSIKRYIRKTAKGTDTATPVFLGALTVLIPCGVTQAMMATALGTGSAGMGAAL
                     MFAFTLGTSPVFFIVAYLTTELGSKLEKFFMRFVAVVVLILGFVTLDGGLNLMGSPLS
                     FQNLTRNLLPSENNSTTQAVSSLPAAEGEIVLEVQNSGYFPTTLKAPAGKELTLNLIT
                     DQTYSCARDFVIPSLDYYQLLPETGTTQVNIPAQEQGSTLFFTCSMGMYTGQIVFE"
     gene            29683..29895
                     /locus_tag="PL000_07705"
     CDS             29683..29895
                     /locus_tag="PL000_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNC88821.1"
                     /translation="MLKKTFKVEDMTCANCAMKLEALEDTLEGVKEINASYHRLEMVI
                     EYDEAKLTDEQIVAAVKKKGYTAVVK"
     gene            30028..30411
                     /locus_tag="PL000_07710"
     CDS             30028..30411
                     /locus_tag="PL000_07710"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HNC88822.1"
                     /translation="MPKYYTLHEANKTLTIIEPMLQEMMEIAMKIRENQPELWDLVRK
                     SAGNGGNPHLSRLLVEFDHLDALFHQLQDMGVVVKDLIIGLIDFTALHNGREVYLCWK
                     YGEGAIQFWHEIEAGFAGRQRIDWE"
     gene            30480..31313
                     /locus_tag="PL000_07715"
     CDS             30480..31313
                     /locus_tag="PL000_07715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC88823.1"
                     /translation="MTVSTIGRYQVESEIAQGGMGVIFLARDPYIQRQVVVKVLMYSR
                     TLEDVYREFFQQEAEVIAALEHPCIVPIYDFGWHGEQPYIVMRHMSGGSLDDHLRKAE
                     VKLTDMAHILKRVSDALDAAHARNIIHRDVKPSNILFDSTGEAFLSDFGIAKSQKISD
                     EDGEEYMVGTPAYMSPEQIKGGRLDGRSDVYAMGVVLYRLLTGQLPFSSNSTTGLINA
                     HVNMPVPDVRLVKANIPAVWQEVISKAMAKDPSDRYATAGDFARDVNEVVSGKWFLRK
                     L"
     gene            31494..32393
                     /gene="rarD"
                     /locus_tag="PL000_07720"
     CDS             31494..32393
                     /gene="rarD"
                     /locus_tag="PL000_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HNC88824.1"
                     /translation="MKKGILYGIGAYLLWGFFPIYWKFLHVVPALQVIGHRIAWSFLV
                     LGVYILLTGQWRDFRKVASNAKTIGIYAIAGVLLSINWLIYVWGVNAGYIVETSLGYF
                     INPLISVLLGVLFLRERLRPAQWLPVFIAAVGVFYLTFVYGRLPWIALSLAFSFGFYG
                     LVKKLAPLGSLYGLTLETGIVFPAALVYLIIAQAGGSGAFMHDGLQVDLFLMGAGIVT
                     TIPLLMFASAAKEIPLSMVGILQYIAPTLQFLIGVFLYHEPFDQSHLIGFGIVWVALV
                     IFWVENYIANRNPVEPIPELGEG"
     gene            32556..33749
                     /locus_tag="PL000_07725"
     CDS             32556..33749
                     /locus_tag="PL000_07725"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNC88825.1"
                     /translation="MANKKGVIGILTGGGDVPGLNPAIRAVTIRALREGYQVVGIRRG
                     WAGLVDIIRDKNADNSNNYQFLSEEIVNRAGRTGGTFLHTSRTKPSSVKKASVPEHLQ
                     GQYAEEKNDLTPEVLKNLAFLGVDYLIPIGGDDTLSYGVRLYKEGVKVIAIPKTMDND
                     VPGTDYCIGFSTCVTRTISLTNSLRTIAGSHERFLVLEVFGRYAGFTAMLPTMAGAAN
                     RCVIPEYKFNIEHLTELMSKDRDANPSRYSVLLISEGAMYEGGEMVFEDRTTDAYGHA
                     KLGGIGDLVSSQIKEISPKFNKGKTVDVVNQKLGYMVRGGDPDAIDSIVPMAFGNLAL
                     DLLLKGVHGRLVVLKNGRYDNMPLDVVTASKKVVEVKHFYDTDRYRPIYENFEMKPLF
                     IMTSE"
     gene            33980..35005
                     /locus_tag="PL000_07730"
     CDS             33980..35005
                     /locus_tag="PL000_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNC88826.1"
                     /translation="MNFAMWKKALNVIPEVSKEEWDQLDVISRWLISTRAAVLIMTFI
                     SAALAGLFAWRDGSFSFIPWIALVFGLIMAHASNNIFNDYTDFVRGVDKDNYYRTMYG
                     AQPIASGLMTQRQHLTYFAVTGFLALVAGLYLIFSNANDPIIWMLLAAGAFFVLFYTW
                     PLKGLGLGEVAVLVVWGPLMIGGGYYVLAHEWNWSVAIASLPYVLGVTTVIFGKHIDK
                     ISVDKAKNIHTLPVVIGEKAARYAVIAMMVLPYIFTGYLIATKFFTPVMLIVLLAIPS
                     LRESLPPLSKPKPDTRPEGFPDGQGGWPLYFAPIAFRNNRSFGSLFMLGLLVDVALRI
                     FLPAFWR"
BASE COUNT         8354 a         8600 c         9494 g         8610 t
ORIGIN      
        1 ttcgtgaagg cggtctcacc gtcggcgcgg gtgtcgttac caagatcatc gagtaggtga
       61 gatgatgatg gctaaacaga aaatccgaat tcgcctcaaa gcatacgatc atcgcgtgct
      121 tgaccagtct gctaagcgta ttgttgaaac cgccgagcgt acgggagccc aagttgttgg
      181 gcccgtaccc ttgccaagta agattgaacg ctttacaata cgtcgctctg cattcattga
      241 taaggattcg cacgagcact ttgaaatccg gacccataac cgtctaattg atgttctgga
      301 tccagattcc aagacgattg atatgttgat gcgtttgaac ctgcctgcag gtgttgatat
      361 cgagattaag atctaatatt ttgtcagtaa ctgtagaata atttctacag attatttgag
      421 acagcgcaag agtaggtctg tgttgtagaa ccgacacaca ccgcttcgca ccagaccttc
      481 tgactgcgct gcacggagat gatgcggtcg ttgccccgac tggcagtctc gtcaatatct
      541 tctaaaggag taaattgagt atgttgaaag ggctaattgg taagaagatc ggcatgaccc
      601 agatcttcga tgagcagggc gttgcctata cggtaaccct catcgaagct gggccatgct
      661 acgttaccca ggtacgcaca ccggagaagg aaggttattc atcggtgcag ctgggttttg
      721 gcgaagtgaa tcccaaacgc cttacgggtg gacagttggg tcattttaaa gccaatgaaa
      781 tttcccctgt gcgctttctg cgcgaatttc gagcaaaaga tcatggtttg aaagtcggcg
      841 acaaagttac agttgtcgac gcttttgccg ttggcgagcg tgttgatgtg gttggcgtta
      901 gcaaaggcaa gggctttgcc ggtggcgtca aacgctatca ctttcacggt atgaacgcaa
      961 cccacggtac atctgaccgt aatcgcgccc ctggttctcg tggttcggga actacaccag
     1021 gccgtgttta caaaggtgca cgcggcgctg gtcacatggg cgtggatcgt gtcaccgttc
     1081 agaacatcaa agttgtcatg gttgatgcgg aacgcaatct gcttgccatt aatggtgcag
     1141 ttccgggcgg caagggtagt ctcgtcatga tcaaggaatc gcgcaagcaa tagcgcgtag
     1201 gaaacgggag ccagattaac catgaaagta gatgtttttg atttgaaggg ccagaaagtt
     1261 cgcgaaatgg aattgcccgc gaatgttttt gaagcccctg taaatgtcga cctgatgcat
     1321 caggcatatg tccgtcagat ggcaaatgcc cgtcttggca cacatgaaac aaaggttcgc
     1381 ggagaagtcc gcggtggtgg tcgcaagccg ttcaagcaaa agggaaccgg tcgtgctcgt
     1441 caaggttcca ctaacgctgc tcagtgggta ggtggtggtc gtattcatac cccccatcct
     1501 cgcagctacg aacagcgtat gcccagaaaa atgcgccttg ccgcccttcg ctctgtttta
     1561 tctgccaaag cggccgatgc cggtattgtt gtagtggacg atctcgatat caaagaaccc
     1621 aagacgcgtg tcatggctga agcgttgggt aatcttgtcg gttcatcaac cgcattggtt
     1681 ttgatgcccg tcaaggatca gagctatgac aatgtcatgc gttctgccag caatattgag
     1741 agcgccaaga tcttactcgc aagttacttg aatgtgcgtg atgtccttaa tttcgacaag
     1801 gtcgtcctgc cgctcaaaac aattgatgcg ctcgtggcgc acctcggata ggagagagac
     1861 atgaccacga ttcatgatgt cctcgtccgc cctttggtga ctgaaaaatc cagcttccag
     1921 tcgggcaaac ttggtcaata cgtttttgtt gtcgctgaca aggcgacccg caccatggta
     1981 aaggacgcag tggaaaccct gtttgatgtt accgtagcgc gtgtaaacat tctgaatgca
     2041 cctgccaaac gtggccgccg aactcgtagc cgccgcttgc tggtgcgccg tccttcgttc
     2101 aagaaagcca ttgttacttt ggccgaagga agcaagtccc ttgaaatctt tgaaggggtg
     2161 caataatggc agttaaaaag tacaaaccag taacccctgg catgcgcgac atgaccgggt
     2221 atacctttga ggaaattacc aaaagcactc ctgaacgttc ccttttggta aaaaagacag
     2281 cccgtggtgg tcgtaacgcc cagggccgcg tcactgttcg gcatcgtggc ggcggcgctc
     2341 gtcgctacat ccgtatcgta gatttcaaac gcgaaaaaca tggcattccc gccaaagttg
     2401 ctgctgttga atatgatccg aaccgcaccg cgcgtcttgc attgcttttt tatgcggatg
     2461 gtgaaaaacg ctacatcatt tcgccccttg acctgaaggt cggtgatgcg gtcatgtctg
     2521 gccccaatgc cgaaattcgc cccggcaacg cccttccaat tagcaacatt cctgtgggta
     2581 ctttgatcca caatatcgaa gttaaggaag gcaagggtgc ccagcttgtc cgctcagccg
     2641 gtggagctgc tcaattgctt gcaaaagaag gcgattttgc gcaaatccgc atgccctctg
     2701 gtgaagttcg cttgatccat caggtttgtt atgcaaccat cggtcaggtt ggcaatcttg
     2761 atcacggtaa cgtcaaactc ggcaaagccg gacgcaaacg ccacctgggt atccgcccaa
     2821 ccgttcgtgg aactgcaatg agcccccgcg accatcctca tggtggtggt gaaggccgcc
     2881 agcctgttgg tctccctggt cctaagagtc cgtggggtaa acccactctc ggcaagaaga
     2941 cccgccgaaa caagaagact gaccagtaca tcgtgcgccg tcgcagtaag acgaaccgct
     3001 aagaattaga cgaggtacgt atatatgtca cgatcattaa aaaaaggtcc ttacattgag
     3061 ccaaaactgc tcaaacgtat tgaggccatg aaccagaaaa atgaaaagaa agtcatccgc
     3121 acttggagcc gcccatctgt gattttccct cagatggttg gacacaccat tgcggttcat
     3181 gacggacgcc gccatgtacc gatctacatt accgaaaaca tggtcggtca ccgcttgggc
     3241 gaattcgccc cgacacgtac cttccgcggt caccagacca aagaagctgc ggcataggaa
     3301 gttgagccat gagcgatatt cacgcaaaac tcagccacct ttccctctct gcgcagaagg
     3361 tccgcatcgt aattgacatg gtgcgcggca agagcgccaa tgaagccctg gaaatccttc
     3421 gcttcgtaaa caaacgtgcc gcattgcctg tgcaaaaact ggtcgcttct gcagttgcca
     3481 atgctgaaga aaactttggc gtcagccgcg acgacctgta tgttgccaag atcacagccg
     3541 atgaagctcc cactcgcaag tggcgtcgct tcggtgcccg tggccgtttc aaaccgatat
     3601 tacgccgtag ttcacatgta actgttgtgt tgcgtgagcg cggacgcgaa gcgtaaggag
     3661 attttatggg tcgtaaagta catcctgttg gttttcgttt agggatcaat cagccctggg
     3721 gtggtcgttg gttcgctgaa ggtagcacct atcgtcagca actacaccag gatctggcca
     3781 ttcgctccct gttacttggt aaattctcca agggtggcgc ggcagctagt gaagttgtgc
     3841 gcggtctccg caagcagatg cccgatgctg tcaaagacgg caaagcaggt attgcccgtg
     3901 tagacgttga gcgcaccccc ggcaaggtcc gcgtttcaat ccacaccgcc aaacccggca
     3961 ttttgattgg ccgcaaaggc gaaggtgtga aaaagattcg ccaggctttg gaaacccttg
     4021 ctggcaaaaa ggtcgagctg gatatcaaag agatttcctc ccccgacacc gacgccatgc
     4081 tggtggcaaa taacattgct gatcagcttg aacgccgcat tgcatatcgc cgcgccatga
     4141 agcgcgccat tcagcaggcc atcaaacaag gcgctgaagg catcaagatt ttggtcggtg
     4201 gtcgcttggg cggcgcggaa atgtcccgcg atgtgtggtt gcgtgaaggc cgcgtgcctt
     4261 tgcagaccct gcgcgccaat cttgatttcg ccactacaga agcattgacc acctatggtc
     4321 agatcggtat caaggtctgg atctacaagg gtgagtctgc acctgaagtt gaagaaaaag
     4381 ctgaagcgac ggaaggcgtg tacgtcagcg agtaatcgcg ctgtcgcatg agatgaggta
     4441 attgatatgt taatgccgaa gcgtgtaaag tggcgcaagc agatgcgcgg tcgtatgagg
     4501 ggcaaggctc tccgcggtgc ggaagtttcc tttggcgaat atggtctgca ggcgctggaa
     4561 ccgggctggg tcaccgcccg tcaaattgaa gcggcccgcc gctccattgt ccgtggaatg
     4621 aagcgccgtg gaaaggtctg gattcgcatt ttcccatcca agccttttac ccacaagccg
     4681 cccgagactc gtatgggttc cggtaaagga aatgtggagt actacgtggc agtagttaaa
     4741 cctggccgcg ttatgttcga agtgggtggc ttgcctggtg atattgctgt ggccgccttg
     4801 aagagcgcaa tgtataaatt ctccattaag accaaggtcg tgggtcgcca cgcggaagga
     4861 gaataaagat gaagtctatg aaaatagaag acatccgcaa aatggctgcg gaggaaatcc
     4921 gcaaaaaact cacagatacg cgcgatgaat taatgaaact gcgctttcag caggtgagtg
     4981 gtcagttgac cgataccagc agcctgccag ccctgcgccg caacatcgcc cgcatggaaa
     5041 cggtcttgcg cgagcttgag aacgctgttg tggaaggtgc ataatgaata atcgtcgtcg
     5101 tatgactggt gtagtgacca gcaataaaat gaccaagact gttgtggtgg agatcacccg
     5161 caagtttcgt caccctcttt ataagaaggt ggtgtactca agcgcacgtg taaaagcgca
     5221 tgatgaaatc ggctgccaga ttggtgatga ggttcagatc gtggaaaccc gtcccttgtc
     5281 tcgtgataag cgctgggcgg ttgaaaccat cgtcaagcgt gagacacgca ccgctgatca
     5341 aggtgtagga gaataagcca tgatccagca agagtctcgt ttaaagattg ccgataatac
     5401 cggcgcccgc gaaatacttg tcattcaggt gttgggcggt tccagacgca agtatggagc
     5461 cattggtgat attgtggtgg caacagttaa ggctgccgca ccccaaggct ctgtgaagaa
     5521 gagtgaagtt gtaaaggctg tcattgtgcg ctgcaagaag gaatggcgtc gcgaagatgg
     5581 ctcatacatc cgctttgatg ataacgccgc ggtcattctt gacgctgatg gcgaaaaccc
     5641 gaggggcaca cgcatctttg gacctgtggc acgcgaattg cgtgacaagg gttacatgaa
     5701 gattgtgtcg ctggccccgg aagtgctgta ggagcgaaga atgaaagtta agattcgtaa
     5761 aggcgataca gtagaagtga ttagcggccg ccttgaagat aaaggcaaac gcggtgaggt
     5821 tattaacgtg atccttagtg atcgccgcgt ggtcgtgcag ggtgtgaaca tccgcaccaa
     5881 gcatcagaag caggtccagg caggtaatcg ccaaatgaac cctggccgta ttcagttcga
     5941 gggaccggta gacatctcaa atgtgatgct ggtttgcccc aaatgcagtg aagccacccg
     6001 tgttggaatt caacgcgatg atgatggtgc ccaccgcgtt tgcaagaact gcgatgcttt
     6061 gatcgattag gccgtggagc agataatatg aacaagatgc aagaactcta taacaatgaa
     6121 gtagccccgg ccctgttcaa gacgttcggt ttcaagaatg tgatgcaggt cccccgcatc
     6181 gaaaaggttg tgttgaacat tggtctcggc gaagcgatgg ataacccgaa agctttggaa
     6241 gccgccaccg ctgatttgat gcagatcacc gcccagaagc ccgtgcagac aaaggcgcgc
     6301 aagagcattg cgaacttcaa gttgcgcgaa ggacgcctga tcggtgcgaa ggtcaccctg
     6361 cgtggacccc gcatgtgggc ttttattgac cgtcttttca acattgcact gccgcgcgta
     6421 cgcgatttcc gcggtgtttc ggcgaacgct tttgatggtc gtggaaatta cacattgggt
     6481 ctcaaggatc agctcgtttt tcctgagatc gaatatgaca aaattgacaa attgcgtggc
     6541 atggaaatca ccattgtgac ttctgcccaa aatgatgatc acgcccgcgc attgttgcga
     6601 ttgcttggaa tgccgttcag cagcaacaag aaggatgctt aatctatggc aaagaaatgt
     6661 atgcaatatc gagaactgcg ccgaagacat gctgttcagg tgcgcaatcg ttgtcagcgt
     6721 tgtggtcgcc cgcgtggttt tattcgccgc tttggtctgt gccgtatttg tttccgtgaa
     6781 ttggcactga cgggcaaaat ccccggcgtc gtaaaggcat cctggtagcc ccaggtggag
     6841 gaagatcatg tcatttactg atccgatcgc tgatatgttg acccgcattc gcaatgctgt
     6901 gctggctggc catgctcagg ttgcaatgcc caactcaaag attaaagtag agatcgccaa
     6961 gatcctcaag gatgaaggct ttctcgaagg tttcgaagtg gtggaaggtg agcgtgaagc
     7021 ccagaaggtt ttacgcgtga agctgaaata tgttggcgaa cgtcgccagc gtaaggcggt
     7081 actttccggt cttgagcgcg taagcaagcc gggccgccgc atctacacca agaagacgga
     7141 cattccgtgg gtgctttctg gtattggcgt agccattatc tccactccca agggtgtcat
     7201 gaccggcgca cgtgcccgac agttgggtgt gggcggcgag atcatctgca aggtgtggta
     7261 ggaggtttat cgtgtctcgc attggtcgat tgcctattga tattcccagc ggcgtacagg
     7321 tgagcgtgaa cggtttggat gttcatgtga agggcccgaa gggtgagatg aaacgatcgt
     7381 tttcccgcat ggttggcatt aagatggaaa acaatcagtt gaacatcact cgcaactcgg
     7441 aagagcccgc cgaacgcgcc atgcatggta ctgcccgagc cgtgattgca aatatgattc
     7501 acggtgtcag caagggtttt gaggttgttc ttcaggtgga aggcgtgggt taccgcgctg
     7561 aaatggaagg caagaatctg gctctgtttg ttggttattc acacccgatc aaaatggaac
     7621 ccccggtggg cgtgacattt gaaacagatg caaaaacccg tcagattaaa gtttcgggtt
     7681 acgacaagga attaattggt caggttgcag cgaatattcg caaggttcgt ccccctgagc
     7741 cttatcacgg caagggtttg cgctacctcg gtgaacgtgt tcgccgcaaa gctggtaaag
     7801 ctggaaaagg agccaagtaa gctatggcta ataaaagtcg ctctctcgct cgcgaacgcc
     7861 gtcatggacg tgtacgcaag catgtttatg gaacttcaga tcgcccgcgc ttgaatgtgt
     7921 tcaagagcct ttctggaatc tatgcccaga tcattgatga taacgaggga aacaccctgg
     7981 tttcagcatc cacagttgat aaagaactgc gtgagaagat gaaggggttg aagaagaccg
     8041 aacaggccaa ggccattggt aaagcagtgg ctgaacgtgc caagtccaaa gggatttctg
     8101 caattgtgtt cgaccgcgga ggtttccgct atgttggacg cattaaggcc cttgctgacg
     8161 gtgcccgcga aggcggtttg caattctaga agttgtaaca acgggttccc gttgttgcgt
     8221 tactggagaa agatatggca gaaaagcaat ttactgaaaa gcagcaattt gaaacacagg
     8281 atgcctttga agagcgcgtt attgaaattg cccgcgtcgc taaagtagtc aagggtggcc
     8341 gtcgcttccg cttccgcgta acagtggttg tgggtgataa gaatggcacc attggtatgg
     8401 gcattggaaa ggcaaatgct gttccggatg ccatgcgtaa ggcatctgaa cgggctcgta
     8461 aagatttgcg tgtagtaaat ctttccaaca ccaccattcc gcacgaatcg atcggcaaag
     8521 ttgcaggtgc aaaagtgttc ctcaagcctg cctcggctgg tacgggcgta atcgccgctg
     8581 gtggcgtacg tgctgttttg gaactggccg gtgttcgtga tattctcacc aaatccatgg
     8641 gcagcgccaa cgttttgaac gtagtgatgg cgacctttgg tgctcttgaa ggtctgcgtt
     8701 cacctgcaaa tgaagcggcc cgccgtggca aacgtgccga agagttggta cccttctggg
     8761 agcggaggaa gcaacatgcc taagcaaacc acctctggca aaaccctgcg tgtgacttgg
     8821 gtgaaaagtg atattggctt tcccaaggat caaaaggcaa cccttaaggc gcttgggttg
     8881 cgcagattac atcaaactgt tgagcataag gatacacccg cgttgcgcgg catgttgaac
     8941 aaggttgtcc atttgctcaa gatcgaagag taggttgaat tatgaaactt cacgatctcg
     9001 tacccaatgc tgggtcaaaa aagaatcgaa aacgtgttgg tcgtggtatt tcggcagggc
     9061 agggtaaaac tgctggccga ggcaccaagg gtgcaggcgc acgttctggc gagggcggtc
     9121 acgcttatcg tcaaggtggt aacctgcctt tcttccgtcg gttgccgttt atccgcggtg
     9181 aaggttttac cccgcccaat caggttttct acaatgaagt gaaccttgat caacttgcac
     9241 aggccttcca ggccaatgct gatgtgacgc ccgaatccct cgaacaggcg catcttctcc
     9301 gcgattcccg caaccctatc gttgtgcttg gtcgtggtga gatgaccgtc gctttgaagg
     9361 ttcgcgtcca tcgcgtgagt gctggtgcca aagccaaaat tgaaaaggct ggcggctcgg
     9421 ttgagttgat cgcctaaccc attaaaggat actttgcatg aagagcactt tttcaggctg
     9481 gcattacctt tggaaatcgg atgatatccg acgcaagttg atcgttactt ttgctatttt
     9541 agcgatttat cgtcttgcgg cgaatgtccc tgcccctggt gttaattttg atgtattggc
     9601 ccaattccgg gcttcatctg cgggcagcgg aggcttcctt ggtttccttg atctactctc
     9661 gggcggaaca gtctccaact tttcactgtt gtcgatgggt gtgtacccat acattacagc
     9721 acagattatt ttgcagctgt tgattccaat cattcccgct ttacagcgca gaatggaaga
     9781 ggatccacgt gaggctcgcc gttggcagga aaagtggaca tattaccttg ccgtgccgat
     9841 ggctgctctt tccgcgattg gtcagatcaa tatttttaat tctctgtccg ctcaggcaat
     9901 tgggcagaca attctcccct ttggattgtt ttcatctgac ctctggcttc cttctctgac
     9961 agttttgctg tctatgacag ctgggaccat gtttgctatc tggctgggtg agttgatttc
    10021 agaatacggc attcgcggac agggactttc ccttgtcatc tttgcaggta tcgttgcacg
    10081 catgcccgcg aatcttgtct cccttctttc agatgagtca acccgttggt tcatgttgat
    10141 atttaccatc attatcatta tcttaacggt gttcgcaatt gtgtacgtgc agcagggacg
    10201 ccgtaatgtt ccggtaatgt atccaggccg ccgcatggga aatcgtatgt ccatgccggt
    10261 caagggaaca ctacctttga tggtgaattt gtcaggcatg atccccttga tttttgcaag
    10321 tgccattttg cagttcccag ccattatcgc aagctatttc actagcagcg aaaatgcagg
    10381 tgttgccaag ttttttactg acgttcagaa cttttttggc gctaccagca caaccactgg
    10441 aaattgggga tactggcttc tgtatttctt catggttgta gttttcacct tcttctacac
    10501 tgctgtattg tttgaccagc aaaactatgg tgaaaacctg aagaaggtgg gagcgactgt
    10561 tcctggcgta cacactggtg cgcccacaca gaagtatttg agcacagttc agcgacgtat
    10621 tactctggtt ggagccgtct tccttggtgt ggttgcaatt atgccatttc tccttggatt
    10681 gcttcttcga gcattgagtc tgaatgctgc cagttctcag gctggtttat tcttggtttc
    10741 ctcttcaggc ttgctcattg tggtcggtgt ggttcgagac accttccaaa acattgatgc
    10801 tgaacttaaa ttgcacggct ataaagattc attgcttgtc cggtaaggag ttgtgatgac
    10861 caccttcatc gtcctgcttg gtcctccagg tgttggtaaa gggactcagg ccaagatttt
    10921 ggctgatgca acaggcctgg cgcatatctc ttcgggagat ttgttccgcg aaaaccttaa
    10981 aaaccaaacc gatctgggta agctggcaaa atcctatatg gataagggcg aattggtacc
    11041 ggatgatgtg accatcaata tgattcgcga ccgcttgaag cgggatgact gcaaaagcgg
    11101 cgcgatcctt gatggtttcc cacgtacacc cgcgcaggct gatgcattgg aaaaaatgct
    11161 ttcggaattc aacggccatg tggattcagt tccttatatc actgccgcag aagacattct
    11221 tattgagcgc actggtggac gatttacatg ccgggccagt ggacatattt atcacgaaaa
    11281 gtttaatccg ccatccaaag ttgggatttg tgatgtagac gggtccgagt tatatcagcg
    11341 tgatgacgat aagaaggaaa ccgttacaaa gcgtatccgt gtttatcttg accaaaccat
    11401 gcccttggtt gagtactatc gtacaagagg caggttggtt gaaattgatg gtgatcagcc
    11461 ggtagagcag gtaacacaaa ggctcctggc tgctctgaaa aaataaattg ttgttattgt
    11521 gcagagcgac ttattagctc tgctgaaatg caggttttat tcaatgagtt gggaacgcca
    11581 gatcaaagta aaaagccccg cagagttgaa gatcatgcgc gaagctgggc atatcaatgc
    11641 atcagttctg gctgcagtaa gagaactttt acaacctggt gtgacgaccg ccgacctcaa
    11701 cgcggctgct gaggaagtgc tgaagtcaca cggatgtgta tcgccattca agaattatgg
    11761 tcatccgcca ttccccgcgt ccattaccgt ttcaatcaat gatgagatgg tgcacgggat
    11821 ccctcatcca aaacgaaagt taaaggatgg ggatattgtt tcaattgatt gcggaacaac
    11881 ctacaatggc tttgtcgcag actccgcttt cactgccgga gtaggggaga tatctcctca
    11941 agcgacaaaa ctgctcgaaa tcaccgaaaa cgccttgtat gcaggtattg ataaaatgcg
    12001 caagggaaac cgtacaggtg atgtctcagc agcgatacag aaatacgtgg aagataacgg
    12061 ctttcacgtg acacgtgagt acacagggca tggtgtcgga agaaaaatgc atgaaggccc
    12121 ccaggtacca aatattggta tcccagggac cggtttgcaa ctccgcccgg gattgacaat
    12181 tgccctggaa cccatggtac tcgttggaac gcatgaaacc cgcgttctgc ccgatcaatg
    12241 gacggttgtc tctgctgatg ggtctttgac agcacacttt gaacatacgg ttgctgtcac
    12301 cgaaggagaa cccctcatcc tgactgtcct gtaaccttgc aggcaaccga aaaagtatgg
    12361 acggttcgta aaacaaccag tataattaag tctttggtcg tcagaaggcg accccaagta
    12421 aggagattga attcatgaaa gtatcaccct ccattcgcaa gcgttgtgcc aagtgccgca
    12481 tcattcggcg caaaggtatt gtttacatca tttgcgaaaa tccaaaacac aagcagagac
    12541 agggttagtg tggtgaccta tggcgagaat tgaaggtgta gatctacccc gcaacaagcg
    12601 ggttgaagtt ggactgacat acctttatgg tattggccca acccgcgccc aggcgatttt
    12661 ggcgaacacc aaagtcaacc ccgatacgcg catcaaagat ttaagcgaag cggatgttgc
    12721 cgctattcgc gactatattt ccaagcatta caaagttgaa ggcgatttgc gccgcgaagt
    12781 gcaaatgagc atcaagcgtt tgatcgaaat tggatcatac cgcggtttgc gccatcgccg
    12841 taacttgccg gtgcgcggcc agcgcacacg caccaatgca cgtattcgca aaggaaccaa
    12901 gaaaacggtt gccggtcgcg gtcgtcgcaa gggcgccaag aagtaattct gtccgaaagg
    12961 tataaataaa aatggctcag agtgttcgtt ccacccgccg cccatcaggc gctaaaaaag
    13021 ggaagcgtac cctgtcctac ggacaggtgc atatctttgc ttccttcaat aacacaattg
    13081 ttactgtaac cgacacagaa ggtaatactg tttcgtgggg ttccgccggt tccgctggct
    13141 tcaagggctc ccgcaagagc accccttttg ctgcgcgcct tgctgctgaa caggccttga
    13201 aagccgcgca acagcttggt ttgcaggatg ttgatctttt cgtaaaaggg cctggccccg
    13261 gccgcgagaa tgcgatccgc gccgtccagt ccatgggttt gaaagttcgt tctatttcgg
    13321 atatcacccc ggtgcctcat aacggctgcc gccctccgaa aaagcgacgc gtgtaattta
    13381 atgaggttgt taccatatgg ctaaaacatt aagtccagtt tgtaaacttt gcaggcgcga
    13441 aggcgaaaaa cttttcctca aaggtgagcg ttgctttacc cccaaatgtt cctttgaaaa
    13501 gcgtgatttt gcccccggcc agcatggacg taccgccggt cgcggcggtg gtaaaggaaa
    13561 agggatgggc actggacgcg cttcagactt tgcccgtcag ttgcgcgcca aacagaaagc
    13621 ccgtcgtatt tacggtgtga tggaacgtca gttccgccgc tactacgata ctgctaaatc
    13681 tggtcagagc ctcacaggtt taaccctgct tcagattttg gaatcccgcc ttgataatgt
    13741 ggtgttccgt cttggttttg cctcgagccg ttcacaggcc cgcctgctcg ttgctcatgg
    13801 tcatttcaaa gtaaatggcc gccgcaccga cgttccatcc atgcttctta gtgaaggtga
    13861 tgtagtcagt gtgcgcgaag gctccagcaa gttgacctat ttcaaggacc tgagcgccta
    13921 caccgaaaaa cgcactgctc ccagttggtt gaatcgcgat atcaaagcga tggctggtac
    13981 cttggctcgc atgccagaac gtgctgaaat tgacggaagt ctcgacgaac aattgatcgt
    14041 cgaatactat tccagacgct aatcttccat tttcgctcca ggctggacct gtaagctggt
    14101 acccagcctt gagccttata taaagggaaa ggtgaaccgt gactggtatc atcacgaaca
    14161 tggttatgcc aaaaattgag cgcgaagctg aagctcgaaa ctatggcaaa tttacaatcg
    14221 gcccgctgga gggtgggtat ggtgtgacgt tagggaatgc cctgcgccgc gtcctgcttt
    14281 cctctcttga aggcaccgcc attacatcca ttcgttttgc ggatgtattg catgagttta
    14341 gcgatgtccc tggcgtgcgc gaagacgtta tccagattat gttgcaagtc aaacagattc
    14401 gcatcaaaat ggacggcgtg gatagtgccc gtatgcacct cgaagtgcgt ggagaaggaa
    14461 ccgtaaccgc ggctgacatt gtcaccccgg cagaggttga gatcgtcaac ccagatacct
    14521 acctcttcac tgtggacggc gcaaagacca aacacgacat tgaatttgtt gtggaacgtg
    14581 gtcgcggtta ttccccggct aacgaacgcg ccggacacat gcccattggc gaacttcctg
    14641 tagatgcgat ttacagccct gtaaagcgtg tgaattggga agtttcctct gcccgtgtgg
    14701 gtcagagcac aaattacgat aaattgattt tggaaatctg gacagacgga accatttcgc
    14761 ccgagcgcgc cctcagctcc tccgcccgca ttctgattga ccacctgcgc tacattgccg
    14821 gtatcaacga agaaatgctt gcggtcgcag tggaacgcga atcctctggc agccgtttga
    14881 gcagtgaact ggccgaaacg ccagtggaag ccctcgatct ttctgtgcgt gtcttcaact
    14941 cattgaagcg cacaggcatc accaacgttg gtgacgttct cgaccttctt gaaaaaggcg
    15001 agaccgcggt catgtccatc cgcaacttcg gcgaaaagag tctcgacgaa cttcgccaga
    15061 agatgcagga aaagggcttt atgaaagacg acacatcctc ggagagttag aagatgagac
    15121 attcagtagc aggttatatt ttaggacgca ccaaaagctc acgtattgcc ttgaggcgca
    15181 acctgatcaa acagcttttt acacacgaac gtatcaagac caccgaagcc aaagccgcag
    15241 cagttcgcgg tgacgctgag cggctgatta cgcttgcccg caatagtgcc aatggtaccg
    15301 atgcggagaa gctcagcgcc cgccgcctcg ccatttccaa acttggtgac aaccaactca
    15361 tcaaacgtct gtttgatgag attgccccac gctttgcaac ccgcccaggc ggctacactc
    15421 gtgtgacaaa actcggaccc agacttggcg atgccgctga aatggtcgtc cttgaattgg
    15481 ttgaagaata aagaaacatc tggcgctcag ctaaagccac tcgctgaaag ctgatagcta
    15541 tatggcacgt tacaaagtga tccttgccta tgacggcacc ggttttaccg gaagtcaaag
    15601 acaggcaaac tcccgcacag tgcagggcga gttcgaaaaa gcgctccgca gactgggatg
    15661 gtctgataag tccatcgtta tggctggcag aacagatacg ggagtgcatg cgacaggaca
    15721 ggtggccgca tttgattttg attgggctca taccgaggaa aaactccttc gggcgctcaa
    15781 ttccgacctt ccttctgatc ttgtcataaa aagcctgatc acggccccgg ccgattttca
    15841 cccgcgcttt gacgccgcct cacgcgaata ccgttatcgg ttgttttgcg gaaccatccg
    15901 cgatccgtta cgggaaaagt atttctggcg ggtatggccc gctctcgacg aagatgccct
    15961 taagcaagcc gccgccatct tcctcggcac acatgatttc gccaccatgg gttcgcccac
    16021 aaccccaaaa gggacgacgg tacgcacggt gacgaaagcc gaatggaaga gaatgccgga
    16081 tgatgagtgg cagttcgaag tcagggcaga tgcgttttta tatcgcatgg taagacggct
    16141 ggtttttgtg caagtctctg tcgctcaggg aaaggccccc ctcgaaaaag tccgcaaggc
    16201 tctggacgag aaaggcaaac tgccttcagg actggctccc gcgcaaggtt tgacgctggt
    16261 tgaagtaacg tatggatcac atgactagat tatgaaacgg agtgacgaaa gtgcaacaga
    16321 aaacatttta tccgaaagcc gctgaaattc agcgcgactg ggttgtaatt gatgccgaag
    16381 ggcagacgct gggccgcctt gctgcgcgcg ttgcgcatgt tctgctcggc aagcacaaac
    16441 ccaccttcac acccggtgtg gaaatgggag actttgtcgt agtggtgaac accgaacgcg
    16501 tcagcgtaac cggcaccaag accagcagca aactggaaag caagatctac tacagccatt
    16561 ccggttaccc gggtggtttg aagagcatct ctctgcgcga ccagcttgcc acgcatcccg
    16621 accgcgccct gcgcgatgct gtgtggggca tgcttccgca caaccgcatg ggtcgtgccg
    16681 ttttgaagcg cctgaagatt tacgcgggtc ccaaccaccc gcatgctgtg caaaccgcga
    16741 aagccgcgaa ataaaagagg taagagaata tggctcagta ttacgaaggc attggtcgcc
    16801 gcaaggccag caccgcccgt gtgcgtgttt ccaccggcag cggaaaattc acagtgaacg
    16861 aaaaggaagg cgccgccttc ttcacccgca tgggcgatgt cgaagacatc atgcgcccgt
    16921 tcagtgcggt ggggcaggaa gctgtcaagt atgatgtgag cgccgtggta aacggtggcg
    16981 gcacctccgg acagaccgaa tctgttcgcc ttggcattgc ccgcgccctt cagctcatca
    17041 acgccgaatg gacagccgcc ctgcgcaaga agggcctgtt gacccgagac gcccgcgtga
    17101 aggaacgcaa gaaaccaggt ctcaagaaag cccgcaaggc accgacctac accaagcgct
    17161 aaacctgtgc tgtggtgtgt cacgtatcaa gaattacgtg gcttattcga tcaattaaaa
    17221 cgtaatgcgt gacggctaac gcctgtttcg cattacgttt tctttgttaa ccaaggaact
    17281 taaaatggac ttttcccttg tttctcaaac atttgagact ctccgcatcg caccaccctc
    17341 gaaactgact ctgctcgagg cgcatacgtt atccccggag tttgcgcatg tgccaccctt
    17401 tccacctgat attccagctt tactgacggg gatcgcctcc cgtgaacttg ccttacaggt
    17461 ccgcaccgtg ttacggaccg tttatcccca ggaacatgag gtctttgtcg ttgaagccgg
    17521 agtagccaga gaacatagcc tgagtgagct tgggactggt gaattttccg agcaaacctg
    17581 cctgtatctt ccttcattgg cggcaggtac gtcgttcgaa tcttttgctg agatcgtggc
    17641 gcatctgcgt gcgccggatg gctgtccatg ggataaggag caaacccacc aaacactccg
    17701 aaaacatctg ttggaagaag cctacgaaac cctgtctgcc atggacgaga acaattccga
    17761 cgagatgcgt gaggaatttg gcgacctgct tctgcagatt gtgctaaatt cacagatcgc
    17821 aaatgaggct ggcgaattta ccatgaatgg ggtgattaaa gggatttatg acaagatcgt
    17881 acgacgtcat ccgcatgtgt ttggagatgt caaactcgat ggcgtggatg gcgttttgca
    17941 gaactgggaa aaattgaagg agcaggaacg caaagaaaaa ggggaggatg gcggcaagaa
    18001 aaagggtctg ttggatggtg tctctgcggc attgcccgcc ttgacccagg cacaggaata
    18061 tcaagaccgc gctgctcgtg ttggttttga ctggcccgag atcgaagggg tgttggataa
    18121 gatccgcgaa gagatcgaag agatcaaaaa agcgcagaac ctcgatgaag tgacagccga
    18181 acttggcgac ctattctttg tccttgtgaa tcttgcccgc tggcgcaaag tggatgctga
    18241 atccgctctg cgcggaacca accagaaatt taagaagcgc tttgcatatg ttgaacaagg
    18301 cgcaaaacag cagggtagaa atctttccga catgacgttg gaagagatgg acatcctttg
    18361 gaacgaagcc aaacgtgtgg gaatatagtt tccttatcag actgtataag cccgaattaa
    18421 ccgcttgcct gtagatgatt tgtaaggatt cacggcaggc aagcggtata aaatctctat
    18481 tatggaatct cagctgggaa caccttccat acctcaaaaa cccaaatggg atctgcccga
    18541 tgtttgggca aaaagcgggc attgtcctgc atgcggggct acgaatttaa agatcacaca
    18601 cttgccggat tacccggatt atctgtcttg cgctcattgt caggtttcgt ttgaagtgga
    18661 aaatgggggg cgccatatcc gtttgaagta cattcccgat caattcgaat acgccgatgc
    18721 catgcttcgt ggacgttggg tggcggcggc agatttaccc gcgattttgc gcaaacctca
    18781 acccgttgtg aaggaagcgg atgaacctca actgccaaag cagcccctct cagatgcaga
    18841 aatctggaag cgggcggttg gcatgtataa attgggtaac aagccgcgta tgatccaact
    18901 gacgttaatg cagtctggcg taccccaggc gcaggcggat gccattcttg aaaaattgaa
    18961 gcgactggca gaagaagaca ctcgaaagat gaaccagaaa ttctggatac tggccggtgt
    19021 cgcgcttttg ttgatcttct ctcttgctgg tggatggctg tatgggagtg ggaatctcgc
    19081 cgtgttgatg ggagatgcga cccctgtgcc cacccaaagc ggtccttcag ccgtggacct
    19141 gttgctggat cttgttcctg atgaagccaa gcctgccatc atcgacctgc caaaaacatc
    19201 tgtggatgtc ggccgcggac cgggacaggc atcctgtccg ccgacatcgg ggatggcggc
    19261 agaattattc ggcggggatg ctgccatgtg gcagcaggcg ggcgcttttg cagcctggca
    19321 gatgatcgac ccgatcaact cagccacgat cactgtgcca accgatatga cagccgccta
    19381 tgtggataac aggacgagac aattcctttc ggtgcatggt ccggcaacga tctacaacgt
    19441 gaattttttg gtgatctcct gttgaggctg tatcattttg agggatgggg aatttcaacc
    19501 gccattagat gttcattgta ataaatacca ttgatgcgaa gcgccttggg ttcagtgccg
    19561 tagattttga atccacagcg ctcgtaggtg tgtatggcag agaggttgtc tgtaacgact
    19621 gccagtttga caatgactac atcatgttcg gctgcccaac caaggcaggc atgaatgagc
    19681 atttcggcaa ttcgcctgcc gcgccattca ggttttacgt agacactgat gatcgttgca
    19741 ctatggtgat tcttttttga gttgttgcga acgatcccag ccatgccgat cggctgtccg
    19801 tgatgttccg aaaaaaacaa ggcttgttca tctgcttgaa atgtcaggcg cttttcccag
    19861 tagctttgcg gttggagcag ggtttcttca taatctgagc caaatgcgga ggggtgattc
    19921 ttcagggcct caaggcgcaa ttcacgaaag acttgcaggt ctgcggggtt tatgtggcgg
    19981 atggtgaaat cagtttcgct tgggggcatt aagtttgttt cccaggtgat cggctttagt
    20041 tgcaggtgaa tcttgccagt gcttcttcga atagcggacc agcggtttcg gcttgcgggg
    20101 tatatacctt ccatgcgaag acacggtcgc cacaggtcca ggcggcgatg gcaccgaagg
    20161 gcacttcgct gatggtggtg gtgatgggga catacatcac gttcatgccg cggatcagtt
    20221 tcacatcaaa ggttccgttt ttggtatcgg cctggtcatc gagcagggtt tcgagcagga
    20281 attgtggatc agatgtgccg ggggcctgca gccagataaa gttaatgacc atggtcggga
    20341 tctgataggc ggcgaggaat ccgttattgt aggcgctggg ggtgagttga ttgagtgcgg
    20401 ccatgtggtc ataaaatgcc atgttgttgg gatgcccaat gcagaattga tattcgcaaa
    20461 acacacctgc gagcgaaatg ggtgtggccg aggggaacgg ggtcggtggg gtgtagggag
    20521 tggcggttgg gattgcggcg agagtttcgg ccacagcctg ttgaatttgg atgttagggt
    20581 cgagggtggg gagagtcgtt ttcggggagc aggctgccat tagaagcagg atcaggcaga
    20641 ggtttgggaa aagaagcgct gtttttttca tcccaaaatt ataaccgccg catttgaaaa
    20701 acagccccgt ccatttctga acggggctgg gtgtttttag tgtccgctgc cgggggcggg
    20761 tgtgccatca aagatttcgg gattgggatc gccttcgacg atgaggaatc cggcgaggcc
    20821 gcgctgcaat cttgagaggg catggtctac gagaatgaag cgacccggga cttcaagttt
    20881 gaattcgacc atggttgctc caccgggcgg gacaagggtg gtttgtacgt cggtgagcgg
    20941 agcagaggtg atggaggctt gatcgaagac acggtcgaag atttcgccga tgacatggaa
    21001 ggaggaggtg aggttggggc cgccgacacc gaagaagatg cggatggttt caccaacgtt
    21061 ggctttgagc ggtttttggt cggtgagtgc gccgaccgcg ccgttgaaga cgacgtattc
    21121 ggggtcttcg ttcaacaact tggtgacatc ggtggtttgc attccgtggt cgccgtaggc
    21181 tccggtggtg tagatatctc cctgcatgac gtagaactcg cggtcaacag ggggaaggcc
    21241 ttcttcgggt tcaacaagga tgagaccata catgccgttg gcaatgtgat tggccaccat
    21301 cggtgtggcg cagtggtaaa catacaaacc cgggttgatg gctttggcag tgaaagtggt
    21361 ttctttgccg ggttcggttt gggttacggc agcaccgccg ccaggacctg tgacagcatg
    21421 gaaatcaacg gagtgattca tggtgcttga gccgctgttc ttcaagtgga cttcgatggt
    21481 atctccaacc cgcacgcgga tgaaaggccc gggcactgca ccgttgaagg tccagtaggt
    21541 gaaggtggtt ccatcagcca gctggccttc gacttcgatg gtttcaagat caacacggac
    21601 ggtctgcggt tcacggactg cgagtggggc gggcaaatca gttgggtcac ggacaacatc
    21661 tgcaccagtg gttggggctt gtggagctgc tgcttcggtt gaagtgctgt acgcgccctc
    21721 tggtaatccg ctcagataat taacaatgga attgatttct tcttcggtga gagcgtcttt
    21781 caaagtggca ggcatgacat tgggcgcgca agcgccagtg gggcaggtgg gggccacaaa
    21841 gagattcggg tctgcaatgg attcgcggat gtattcttca gtggtggtgg ctgtgccggt
    21901 gtaggcatcg cttttcaaat gttcttcagc ggcggtgttg atatcggaaa gattcggtgc
    21961 gatcacgccg accgcgttgg ggattccatc aatggcatgg cagcttccgc aggcgccttt
    22021 ttggaaggca gccaatactt gtgggtcacc gttgctttgt tcggctgcca cgggcatggg
    22081 ttgatcggtt tcactcacca tcaacttgcc gcgcattccc aattcagcgt ggttggctac
    22141 atcgtcataa tattccattt ctccgtgggt gtttggcacg gtgaatgtca cggaggcttc
    22201 ttcacctttt tctgtgagga cagcggtgct ggcctgcact tcggggaagg tgaggttgtg
    22261 ctgaccttcg ccgccattga tcaatgtgac ggtgatggtg ctgccgggac gggcgctcag
    22321 ggttgggttt tcaagtccgt tgatctcatc gctgacgcca agaaagacca gcctgccttc
    22381 gcgcaggtcg gtggtcagca cgtattcctt gtccacgggt tgaaaacttg tccctgtaca
    22441 ggcagagacc aaaataccaa gaaaaatggc tgcaacaaga aacttcagaa atgttttcat
    22501 tttttactcc ttattaaggg ttttgaacgt tggcttgttt cagttcttcc agtgcttttt
    22561 gaccatcaag ctggtaggtt ttgaccacat ctttccaggt acaaaaacgg gcaaaaccac
    22621 agcccacaca acctgtgcgc cagtttaaaa aaagcctggc ggccccgggg atggatctca
    22681 aaatgtcttc aacactggct tcagaaattt caaaactttc gttcatggtg acgatagaat
    22741 atcgcactcc cccaaacctg tctttgcgct ggcgcaaatt ggcgtaagta aaaaattacc
    22801 tcctactttt cgagtccgtc tgcaatgcgc accagatcgt gcggtttgat gatgcggatt
    22861 cgctcacggc cagtggtgat gatgccctgc cgttcccagt cactgaacag acggctgacg
    22921 gtgtacagcg tggttccagt catctctgcc acatcctgtc tggaaaagga taattcgatc
    22981 gccgcatctt tttcggacct gatgcctgcc tgccctgcca ggcggaccag cgcattggcc
    23041 actctctgct caacacgttc cgtggcgagt tcgcggtagc gtgcctgcat ttcctgaatg
    23101 taggtggtca tgagggtcat cagatcgaaa gacaactgcg gacgtgtttg gatcatttca
    23161 tgcagaaatt cacttttgat ggccaatgcc gtgctatttt ccagtgcctg cgcactcgct
    23221 gggtaggtgg cctccgcacg gaccgctccc aacgcaccaa acatttgcca gggataaatg
    23281 attcgcaaat tgacctgctg cccgttcaca gaattttgca ttaactttac ctgtccgctg
    23341 gtaaggatgt aaagataatg agcggggtcg ccatgaaaaa agaagaaacc actttcttcc
    23401 attgaacgtt ccacgctgtt ttgcaaaatg agtcgcaggt catcgtctgt tgcattctgg
    23461 aaaacgatca cctgtttgag gtcttttggt gaaagattca tatttccatt ttacacccca
    23521 ttctctttga tgcagcgcaa agagaaaaaa ggcccttttg agtatattga tgttgaaaaa
    23581 ggacttctta tgaaaaaaat ctttgttgtt ttaggcgttg tattcatgat ggctgtactg
    23641 gccatttgga aatatcagca ggttgaactg tatcaattcc atgggtcggt ttttcgggaa
    23701 cccaaacttg ccgctgagat cgttttacgc tcggcaagtg gcccggtgcg tttggcggat
    23761 tatcgcggaa agataaccct gctttacttt ggttacactt cctgcccgga tgtatgcccc
    23821 accacgctgg caaacttgaa attggcactg gagaatctgg ctcccgcaga tgcatctcag
    23881 gtgcaggtta tttttgtctc tgtggaccca gaccgcgata cccccgaaag gctcggaaaa
    23941 tatgtccagt tgttcaattc aggctttgtc ggcgctacag ggactcgtca ggaaattgac
    24001 ctgatgaccg atgccttcga tgtgtattac aagatcaata attccgagtc aaaagagaat
    24061 tacagtgtgg atcattcggg ttttgttacc gtgatcgaca ggcatggcta tgctgtcatg
    24121 agttggcctc atggaaccgc tccccgcgat gtaagcgaag atctgcaata cttgatcgcg
    24181 catgatatgc cagtcagtgc gcaaattttg gcaggaccaa catacacccc ggtagtgtgt
    24241 gccgttaccc tggctcctgc acatattcaa ggcggagagt ggctgtatca acatcactgt
    24301 gcgcaatgtc atggcgctga tttgggcggc aacgcctcct ggcaggtgga attggcagac
    24361 ggcacccacc tgcctccacc tttggatggc aatggaaagg cctggcaata ttctgaaacc
    24421 gagttgatcg agatcataaa aaacgggcgt aatctggata agccgttgtt tatgccagca
    24481 tttaaaacca cgcttgcaga ttgggagatc agttttattc tcacttatct caagagccat
    24541 tggagcatta atcaactgaa ctaccagcac ggctttatga ccctaacccc gcaggcaacc
    24601 ccatccactt tgccgaccaa tacagcactc ccataatagg aattttttct gttagaccac
    24661 acacggggaa attggttgcc gtgttggatt gcatggataa aaagtgacat tcctctcctt
    24721 gacagaacct ctgctgcaat ttatcatttg aacaccctac ccccctgggg ggggtagaaa
    24781 gcagaggtga tgatggaaaa cgacaacaca ctacgaagat taaagaccgt tgaaggccat
    24841 ttacgcggag ttatccgcat ggtggaggaa gatgcttatt gtatcgatgt catccgccag
    24901 attcaggcga tcgaagcggc tttgaacaag gttagctcga agatattgga agatcatctt
    24961 aactcatgtg tcattaccgc cattcagggc agtgacaaaa aggaacgtga gcgtgtgttg
    25021 aaagagatca ccgaagtgtt cgaaatgtcc accaaagttt aagagttgac aagttgctgg
    25081 cagccgcttt ttgcccaaac cctgcaaact tgaaacccgt aaatccgaaa caaataaaaa
    25141 ggagtaaata ttcatgacca cagttaccta taccgttccc gccatttcct gcggacattg
    25201 cacccgcacc atcgaaaccg aagtctctga cttgcagggt gttcaaaccg taaaggctga
    25261 gatcgaaagc aagaaagtga ccatcacttt cgatgccccc gcagatgaaa caaagatcaa
    25321 agctttgctg gcagagatca actaccctgc cgaaggcctg ctgacactgt aaacctcaga
    25381 cctgccgggc tttgaagcct gacaggtcat tttttttcaa ggaatttcaa tgaccgacac
    25441 caagcaactc acccttccca tcaccggcat gacttgcgcc aattgtgtgg caacagtcga
    25501 acgtaatttg aaaaagctgg atggtgtaca aagtgcggtg gttaatctct cctctgagcg
    25561 tgccactgtg gattttgatg ccgccaaact ggggctgacc gacgtcattg cgcgggttaa
    25621 tcgtgcaggt tatggcgttg ccacaggcga agcggatttg atcatcaagc gcctttccga
    25681 tggcaacgat gccgcacggt tggaaaaagc cctcgtcaaa cttgaagggg tgctcgaagc
    25741 acaggtgacc tttgccaatg aaaaagcccg tgtgaaatac attcccacca tcattacgca
    25801 ggccgaattg cgccgcgcgg tttcatcggc aggttttgag gccctggaat tggggggtga
    25861 ggcggaagat gcggaagcac tggctcgcga acatgagatc aacgaacagc gccgcctgct
    25921 tatcattggt ctggtgttca ccgttccact tttcctgctt tccatgagca aggattttgg
    25981 cttgctgccc gatttctttt acaccggcac caccatggat gggatgcgcg atgcccaacc
    26041 ctggttcggt tggttgatgc ttgtgcttgc actgcctgtt caattttatg tgggctggca
    26101 gtattatgtt ggggcattca aagcgctgcg gaacagatct gccaatatgg atgtgctcat
    26161 cgtaatgggc tcttccgctg catttttcta ctccctgccc atcacctttg gctggctgat
    26221 ggggcacgtt tattacgaga ccgccgcggt catcatcacc ctgattaagc tcggcaaatt
    26281 tctagaagcc cgtgccaagg gacgcacctc tgaagcgatc aaaaaactga tgggactccg
    26341 cgccaagacc gcccgcgtca ttcgtgacgg ggtggaggct gaggtctcga ttgacgatgt
    26401 gcgtgtcgga gacatggttc tggtaaagcc tggcgaaaaa attcctgtgg atggcgccgt
    26461 tgtggaagga cgcagcgcag tggatgaatc catgttgacg ggtgaatcgc tgccagtgga
    26521 gaaaaagcca ggtgacgcgg tcatcggggc caccctcaac aaactgggca tgctcaaatt
    26581 tgaagccacc aaggtgggca gggaaaccgc ccttgcacag atcatcaaac tggtggagga
    26641 tgcccaaggc agcaaggctc ccattcagaa aatggccgat caggtttcag ccgtatttgt
    26701 tcccatcgtc atcggcattg ccttcctgac gtttttagcc tggtactttt tcgggccagc
    26761 cctgccgatc aactcagaca ttgataattt cacccgcgct ctcatttaca tggtggctgt
    26821 gttggtcatt gcttgcccgt gtgccatggg gcttgccacc ccaactgcca ttatggtggg
    26881 cacaggcaaa ggcgccgaag cagggattct gtttagaaac agcgaagccc ttgaacgggc
    26941 gggcagggtt tcggtggtgg tgctcgacaa aacgggaacg atcaccaaag gtcaacccgc
    27001 tgttacagac atagttacca ataacgaatc accaatgact ggtgatgaac ttctccgcct
    27061 cgctgcttcc gtagagaagg gctctgaaca cccgttgggc gaatccattt gggccgaagc
    27121 caccacccgt ggattggttt tagtggaacc cgctggtttt aaagcagaag ccgggcatgg
    27181 cgtgcaggcc gaggttgagg gaagaagcgt aatggttggg aacaggcgaa tgttcgaggc
    27241 gaggggaatc cagttgggga atctcgaatc cgaagtgaat cgtctgcaat ctgaagcaaa
    27301 gacagcaatg ttggttgcag tggatggcct tgccgtcggc atcattgccg ttgcagatac
    27361 cataaaagac acttcgaaag acgccattac caatttgcac gaaatgggtt tgaaggtggc
    27421 aatgatcaca ggtgacaacc aaaagaccgc cgatgccatt gcgaaacaag ttggcattga
    27481 catggtgctg gctgaagtgt tgcctgaagg taaatctgct gaagtaaaga aattgcaaag
    27541 tgaccaatct ccaaccaccc atgtggttgc catggtgggc gatggggtga acgatgctcc
    27601 tgcgctggcg caggcagatg tgggccttgc cattggcaca ggcaccgatg tggctatggc
    27661 ggccgcccct gtgaccttga tgagcggcga tttgcgcggc gtgtcacgtg ccattttgct
    27721 ctcacgcaaa accctttcca ccatcaagca gaatttgttt tgggcgttca tttacaacgt
    27781 tatcctcatc cctgccgccg cgcttggata tttaaacccg atgttggccg ctggcgcaat
    27841 ggcttttagc agcgtctttg ttgtgacgaa tagtttgcga ttgcgaagcg tcaagatata
    27901 gcccataggg cagtactctc ccgctttcta ttttctacag gaaaccatta acatgaaaaa
    27961 acttacactc cttcttctcg cagttctctt tctgactgca tgtgctccca aagctgccga
    28021 aatgcccaaa gctgaattgc ctgctggcaa ggcctgggct gatggcaagg aaatttactt
    28081 catccatact gaagcctccg atgcaggtgt ggctgaaaaa ctcaccaaca tgatgaaatc
    28141 ccccgtggtg cttgtgcctt ctctggcaaa cgtgcccgcc gaatcgcttg ccaatgtgta
    28201 tgtcttcacc aatgggttgg caggcacagg ccccttcggc tttcaggctg atgtattcga
    28261 caacccgcca ggcactgacg gatatacccc tctgcgcagt ttgcatctga ttacatggac
    28321 cgatgaaacc aaggcccgtg aactcacttc cgctgccgat gtgctggccg ctgaagctgc
    28381 tggcgaattg accattgaac agcctggcgt tgtcattaac atgcctttcg tcgtctggga
    28441 tggcggcaag cgctaattcc actttccgcc ttttaccttt attgaggacg aatgaagaaa
    28501 aacaagaaca aaccctttga ccccgtcatt atcattaccg tgggagttac ctttctcgcc
    28561 gtcgctttca tgctgttggt caaaccccaa tcacttgcag gcgactcgag tgccatgcct
    28621 cagttgatgg tggccttcgt tacggggctt actacgggcg ggttgagttg ccttgcagta
    28681 cagggcggcc tgctggcgag ttcacttgca caccagatcg aacaggatta cgttgcaggg
    28741 caggctgcaa aaaaagccgc ctcgccccgc accaatagcg ccctgccgat tctattgttt
    28801 ttgatttcaa agatcgcagc ctataccctg ctcggtgcgc ttcttggcat attgggttcc
    28861 taccttaccc tcagcccgct cacccgcgcc atgttgatga tcttgattgg catctttatg
    28921 atcggcaacg cgctgcgcat gttcaacgtc catcccatct ttcgatactt ttcgatcgag
    28981 ccgccgtcat ccatcaaacg ctacatccgc aaaaccgcca aaggcaccga tactgccacc
    29041 cccgtttttc tgggcgcact cacagtgctt atcccctgcg gggttacaca agccatgatg
    29101 gcaaccgccc ttggcacagg cagtgcagga atgggcgccg cgctcatgtt cgcattcact
    29161 cttggcacca gccccgtctt cttcatcgtg gcctacctca caaccgaact gggctcaaag
    29221 cttgaaaaat tcttcatgcg atttgtagcc gtggtggttt tgattttggg atttgtaacc
    29281 ctcgatggtg gactcaacct gatgggttcg cccctctcgt ttcaaaacct gacccgcaac
    29341 ctgcttccgt ccgagaacaa ttccaccacc caggctgtat cttccctacc cgccgccgaa
    29401 ggagagatcg tcctcgaagt acaaaacagc ggatatttcc ccaccaccct gaaagcccct
    29461 gcaggcaagg aacttaccct caacctcatc accgatcaaa cctattcctg cgcccgcgat
    29521 tttgtcatcc catcgctgga ttactatcaa ctcctcccag aaacaggcac gacccaggtc
    29581 aacattcccg cgcaagagca gggcagcacc ctcttcttta cctgctccat gggcatgtat
    29641 acagggcaga tcgtgtttga ataaaaatga aagagacctc caatgctcaa gaaaaccttc
    29701 aaagttgaag atatgacctg tgccaattgc gccatgaaac tggaggcgct tgaagatacc
    29761 ctcgaagggg tgaaggagat caacgccagt tatcacagac tcgaaatggt catcgagtat
    29821 gacgaagcca aactgaccga cgaacagatc gtggcagccg taaagaaaaa gggatataca
    29881 gcagttgtaa aataggaaaa aaagagataa gaaaacaagc gcacaagtaa gaatcgacgt
    29941 gagcttgttt tcttttttca tccttcatcc ttcatccttc atccttcatc cttcatcctt
    30001 tttctccgtt tccgttatac tccccacatg ccaaaatact ataccctcca tgaagcgaac
    30061 aaaaccttga ccatcatcga acccatgttg caggaaatga tggagatcgc aatgaagatc
    30121 cgcgaaaatc agcctgagtt atgggatctg gtgcggaaat ctgctggcaa tggcgggaat
    30181 ccccacttga gcaggctgct tgtggaattt gaccacctcg atgccctatt ccatcagctg
    30241 caggatatgg gagttgtggt caaggacctt atcattgggc tgatcgattt caccgccctg
    30301 cacaatggca gggaggtcta cctgtgttgg aaatatggag agggggcgat tcagttttgg
    30361 cacgagatcg aagcggggtt tgctgggcgt caaagaattg attgggaata gtaagttgcc
    30421 aactcgctca aattgaccta tactagcccc tatctatctt ataaacagcc cgagggaaga
    30481 tgaccgtatc tacaattggc cgttaccaag ttgagagtga aattgcacag ggcggaatgg
    30541 gtgtcatatt tttggcgcgt gacccgtata ttcagcgcca ggtcgtcgta aaagtattga
    30601 tgtatagccg tactctggag gatgtatatc gggagttctt ccagcaggag gcagaggtga
    30661 tcgccgccct tgagcatccc tgcattgttc ccatttatga ttttggctgg cacggggaac
    30721 aaccatatat cgtgatgcgg catatgtctg gaggcagcct tgacgaccac ctgagaaagg
    30781 ccgaggtcaa gctgacagac atggcacaca tcctcaagcg ggtatcagac gcgctggatg
    30841 cggcccatgc cagaaacatc atccaccgtg atgtgaaacc ctccaatatt ctctttgact
    30901 ccacgggcga agccttcctt tcagactttg gcatagccaa atcgcagaag atttcagatg
    30961 aagatggcga agaatacatg gtcggcactc ctgcatatat gagccccgaa caaataaaag
    31021 gcggccgcct ggatggtcgt tctgatgttt atgccatggg tgttgttttg tatcgtttac
    31081 tcacggggca gcttcctttt tccagcaatt ccaccacggg tcttatcaat gcgcatgtca
    31141 acatgcccgt ccccgacgtt cgcctggtca aggcgaatat tcctgcggtc tggcaggagg
    31201 tcattagcaa ggccatggca aaagaccctt cagatcgtta tgccactgcg ggcgatttcg
    31261 cccgtgatgt gaacgaagtg gtttcaggca agtggttttt gagaaagttg taagagacaa
    31321 cataccgccc ccatcaatga ccttgttgcc agaagaacaa ggtcattttt ttgttgaact
    31381 catccatctg tgtagacatt gttggaagga agccgcattc tgcatcctct actggcagag
    31441 gacctgcaag gcttgccacc gatttaagct aatttgtaag ccaaaggaga aaaatgaaaa
    31501 aaggaatact gtatggtatc ggcgcttatc tgctttgggg gttcttcccc atctattgga
    31561 aatttttgca tgtagtacct gctttgcagg tcatcgggca tcgcattgca tggtcgttcc
    31621 tggtgctggg tgtttacatc cttttgacgg gtcaatggcg cgattttcgt aaagtggcct
    31681 ccaatgccaa aaccatcggc atatatgcga ttgcgggcgt cctgctctcc attaattggc
    31741 ttatctatgt ttggggtgtg aatgcgggct atattgttga gaccagcctg gggtacttta
    31801 tcaatccgct catcagtgtt ttgctggggg ttctttttct gcgtgagcgc ttacgaccag
    31861 cgcagtggct tccagttttc attgcggcgg ttggggtgtt ctatctcacc tttgtatatg
    31921 gccgcctgcc gtggatcgcg ctctcgcttg cgttttcatt cggcttttat gggttggtga
    31981 aaaagctggc gccgcttggg tctttgtatg gcctcaccct tgaaacaggc attgttttcc
    32041 ctgcggctct ggtctatttg atcattgcac aggcgggagg cagcggcgca tttatgcacg
    32101 acggcctgca agtggacctg tttttgatgg gcgcggggat cgttaccacg attccgctgt
    32161 tgatgtttgc ctcggccgcc aaggagattc cgctttcaat ggtgggaatc cttcaataca
    32221 ttgcgcctac ccttcaattt ctcatcgggg tgtttttgta tcatgaaccg tttgaccagt
    32281 cacatttgat cgggtttgga atcgtttggg tggcactcgt cattttttgg gtggagaatt
    32341 acattgccaa ccgtaatcct gtggagccca tcccagaact gggggagggg taggatagga
    32401 agaccgttct tggtgtgccg caccttgtct ttgggtaaag gtgcggcaca ctttcggcgc
    32461 tgattcatca tcgaagcagg ggcaggattc aggttaagaa agaggcgcgt tttttacgtc
    32521 gaagatgtat aatgacttca gtcaaagagg tgaatatggc aaacaaaaaa ggtgtcattg
    32581 gaatcttgac tgggggcggc gatgtgccgg ggttgaaccc tgccattcgc gctgtgacga
    32641 tccgcgcgct gcgagagggg taccaagtgg tggggatccg ccgcggctgg gccgggctgg
    32701 tggatatcat ccgcgataag aacgcagaca atagcaacaa ttaccaattt ttatctgaag
    32761 agatcgtgaa tcgtgctggc cgcacaggcg gaaccttttt gcatacttcg cgcacaaagc
    32821 ccagttctgt aaaaaaggca agtgtgcccg agcatttgca gggacaatac gccgaggaaa
    32881 aaaatgacct gacgcccgag gtgttgaaga accttgcttt tttgggcgtt gattatttga
    32941 ttcccatcgg cggtgacgat accctgagtt atggcgtgcg actgtacaaa gagggtgtga
    33001 aggtgatcgc cattccgaag accatggata acgatgtgcc tggcactgat tattgcatcg
    33061 gctttagcac ctgcgtgacc cgtaccattt cgttgaccaa cagcctgcgc accattgcag
    33121 ggtcgcatga gcggtttttg gtgttggagg tgtttgggcg gtatgcaggt tttaccgcca
    33181 tgctgccgac gatggcaggg gccgcgaacc gttgtgtgat ccctgaatat aaatttaata
    33241 ttgagcattt gaccgagttg atgagcaagg accgtgatgc caaccccagc cgctattcgg
    33301 tgttgttgat ttcagaaggc gcaatgtatg aaggcggcga aatggtcttt gaagaccgca
    33361 ccaccgatgc atatggccat gcaaagttgg gtggtatcgg cgatttggtt tcctcgcaga
    33421 tcaaggagat ttcgccgaaa tttaacaaag gcaagaccgt ggacgtggtg aaccagaaac
    33481 tgggttacat ggtgcgcggc ggtgaccccg atgccattga ttcgatcgtg ccgatggcgt
    33541 tcggcaacct ggcgcttgac ctgttgttga agggtgtgca tggccgtttg gtggtgttga
    33601 aaaacggacg ttacgacaac atgccgttgg atgtggtgac ggcgagcaag aaggttgtgg
    33661 aagtgaagca tttctatgac accgatcggt atcgtccaat ctatgaaaac tttgaaatga
    33721 agccgctttt cattatgacg agcgaataac ggttttggaa aacgatttgc cccgtcaggt
    33781 tcacgtggac ctgacggttt tattttcaaa tggggtgaaa cctttgctgt gaagttctgt
    33841 ggatgggatt tttctttccg agtggctttg tggcgaaaaa aggatcttag actctttcca
    33901 aggtaacaaa accgccaatt ccaaagatgg attcaagacc gaattggtta taattgtcaa
    33961 aattttcaag gagtatcaaa tgaactttgc aatgtggaag aaggccctca atgtgattcc
    34021 tgaggtgtct aaagaagaat gggatcagct tgatgtgatc tcgagatggt tgatctcaac
    34081 ccgcgcagcc gttctcatta tgacctttat ttcggcggcc ctggcgggcc tttttgcctg
    34141 gcgcgacggc tcgttcagct ttatcccgtg gatcgcgctt gtctttggtc tcatcatggc
    34201 tcatgccagt aacaacatct tcaacgatta caccgacttc gtgcgcggcg tggacaagga
    34261 caactattac cgcaccatgt atggcgccca acccatcgct tcggggttga tgacccagcg
    34321 tcagcacctg acctactttg ctgtgacggg attcctcgcg ttggtggcgg gcttgtatct
    34381 tattttctca aatgccaacg accccattat ttggatgctc cttgcggctg gcgcgttctt
    34441 cgtgttgttt tacacctggc cgctcaaggg actcggcctt ggcgaagtgg ctgtgctcgt
    34501 ggtttggggt ccgctgatga tcggcggcgg gtattatgtg ttggcacatg agtggaattg
    34561 gtcggttgcc attgccagcc tgccctacgt gctgggtgtg accaccgtca tcttcggcaa
    34621 gcacatcgac aagatcagcg tggataaagc caagaacatc cacacactgc ctgtggtcat
    34681 cggcgaaaaa gccgcccgct atgcggtcat tgcgatgatg gtgctgccat atattttcac
    34741 tggatatttg atcgccacca aatttttcac gcctgtgatg ttgattgtcc tgctggcgat
    34801 tccgtccctg cgcgaaagcc ttccgcctct ttccaagccc aagcctgata cccgtcctga
    34861 aggttttccc gacgggcagg gaggctggcc gctctacttc gcgcccatcg ccttccgcaa
    34921 caaccgctcc ttcggctcgc tcttcatgct cggcctgctc gtggatgtag ctctgagaat
    34981 cttcctcccc gccttctggc gctaacagct tgacctgatc aaagtccctg aaagagaaat
    35041 ctttcgggga ctttttga
//
LOCUS       DAOQLC010000032        34679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_380_length_34679_cov_21.529199, whole genome shotgun sequence.
ACCESSION   DAOQLC010000032 DAOQLC010000000
VERSION     DAOQLC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_380_length_34679_cov_21.529199"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PL000_07735"
     CDS             <1..1146
                     /locus_tag="PL000_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC88827.1"
                     /translation="EGLPVWEWLQQFIDPMHRVVNEREAEIASFLCYAESLLCGTTTV
                     VDMWRHMHGSANAAAQLGIRAVLVPYVAEHPDHDYFETLKTNEALIQKWHGGADGRVQ
                     VWVGLEHMFYATPEAWGRVTAMAKEYQTGFHTHSNESKFDVEETARRYNIRPIEALEK
                     FGLLDAPKTLLAHCVWLNDSEIDILAARRIGVAHNPISNMKLASGAARVVDMLNKGVA
                     VGLGTDGEKENNNLDMFEELKTASLLAKLSALDAAALDAWSTCRMGTVTGARAIGLDS
                     EIGSIEIGKQADLIAVRTDTPRLTPLLSGTAGNLHHNIVHAVRGGDVDMTMVAGNILV
                     DGGQLLTADIRQLIDEANTAVPPLFKRRAEWLASHKSVNELVKHKDD"
     gene            1215..1520
                     /locus_tag="PL000_07740"
     CDS             1215..1520
                     /locus_tag="PL000_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88828.1"
                     /translation="MISMILGTLTVIALLLVVGALIHLKWTETISERTISRRIKAEYP
                     AEVQAQVYQTYQHMKVKELEGLFLKILDDANGDVLKVQKLASIAESVSWRAFLENHW"
     gene            1602..1862
                     /locus_tag="PL000_07745"
     CDS             1602..1862
                     /locus_tag="PL000_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88829.1"
                     /translation="MATLEQEEIALLKGRISRLEARLEFLYKHLNVTFVEDTRLSDDP
                     KVIAALKANNMLEAIKLYRAATNTSLEAAKAGVEEMRARLGI"
     gene            2035..6000
                     /locus_tag="PL000_07750"
     CDS             2035..6000
                     /locus_tag="PL000_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC88830.1"
                     /translation="MPNIAAFVRKVYFPLVSLLCLIGVIGTGALSWRTSQDQDQRQQA
                     SFEFGSDQAERVATEVNAEMNLQMTIAETIAGEISDGTLAYTEITGRLRDELDAHPNF
                     FGMGVAFLPYVYKSDLKLFAPYYFKNEFGNFTRAQVETDYDYSDRATQDNEWFYSALE
                     DGAYWYASEYDITAQAELVEYITPFYRTNPQTGQQEAVGVVWVDHSVETMKSFIRSID
                     VGEDGYTTLIESAGYIVVHPKAEYINKTLYEVAELVGDTELSAAADRAISGEDFYLQR
                     KPTVNLNSTAWTFFKPLIAEGWSIAVTISDNVFEAPPEELLRTQIWLSIMIMTALISL
                     ALLIFRVDEGAVRNLWIVVGWSTFVLLSGVMWMWQIVNTNPSNITASKNILVSTSVVN
                     ESLKSVDETATINGRPIALRIPTGVLVQNIQIQPNEAVISGYLWQKYPKELPPNFMEH
                     LEHEFIQGVEFPDDTGATAFEESYRFQRADSEIVGWFFTTTLHQSYNTAQYPLDQANI
                     QIRISPQYLDQNIVLVPDFESYDFVAPSSLPGLSPQINIPDWNIQRSFFSYQLQQFNT
                     NFGSQTHIQRNNLPTLTFNISASRNLLSPIIAFCITIIVVAGLMFGSLIVKLDSAYSA
                     LSNAAALFFVVAITHVGLRGSIAAGGTVYLEYFFIVLYIFILGLALDGLMYYNESAIP
                     IISYGENLIPKLCFGPGLAITFLAITIFVFYPRTVAADPLQEYLSINALQATEEPAAS
                     DLTPTASPVTDSGSAANNLTATPIRPSTSPAATTVPVILPEDPITLRYPFGLEPPTLD
                     PSLVSLTSDQDQVEQLFIGLTRLESGTNTPLPNLAASWTTSEDGLTWTFHLRNDIPWV
                     QYNAATNSVSQVTDFNGTPRFVNAYDVVYGIHHALTSENTSAALLYEIKNAEQPDDDL
                     GVKALDEWTLEITLNRPAPYFASIVAYDVSYPVPGWAIEEWDKEWIAPENIVTNGPFV
                     LASWEKGKGITLLKNPLWPEADKIQIERVEEILVQDGEIALKLYRDNQIDTLGGLRSN
                     ILQEVKSDPTLANELSSHSNLCVRYFGFTNTKPPFDDPRVRRAFSAAVNRQEIVEMLK
                     VGNIPAFTFAPPGIFGAVTDPGVGQGFDADLAKQLFQEYLDERGFTIADFNSAYPLVM
                     GQLNGLNNSASDIAIKNWNDILGVTVTTKTVETAEFQSSIAKDTPIKNAFSMFQWAWC
                     ADYPDQNDFLHVLFNSETGTNLVRRNCADETCTAILPNDEFDNLTLMASQINDPEERQ
                     RLYAQAEHILVVEEAALAPIYYGASTSLTKPWLTRNFPNMGAPDFMNWIIDILAQKTT
                     P"
     gene            6019..8079
                     /locus_tag="PL000_07755"
     CDS             6019..8079
                     /locus_tag="PL000_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNC88831.1"
                     /translation="MDKSLTPFFNPRGVVVVGASTSPEKLGYGVARNLIESKYRGAIH
                     FVSQKKGELFGHPLLLSLNEVPDPVDLAILIVPTQSTPQTIEDCARRGIKAAIIVSSG
                     FREVGAEGAALEAQCVELARKHGVRLLGPNCIGTLDTHLPLDTTFLQPPMPVKGGIAF
                     VSHSGAFCAAIIDWAREQGFGFSQIVSLGNQADVNETDVLPAVANDEHTKVIALYMES
                     VSNGVKFVQAASEVTRHKPVIALKVGRFEAGQKAAASHTGALAGSEAAFDAAFAKAGV
                     LRADTAEQMFDWAQALENCPLPRGKRMAILTNAGGPGVIAADSLETNGLLLSQLTEST
                     LKALTSSLPPSASVHNPVDMLASASPDAYATCLNLLLQDETVDGVLVILPPPPMYKTE
                     EVAKKIIRVIGNFEKPVVVALMGSTLVENARKEFHAASVPNYPFPERAASALGALAKR
                     AEFFTTESQRHRELKTPRLGASVVNLSADELVSAYGIPTTPIKLARNENEAAAIADEL
                     GFPVVMKIASPDILHKSDVGGVLLNLTTPQTLLSAYTQMMERIKSAMPAAKIDGVHLQ
                     RQIPEGQEVIIGMVRDPLFGPLMMFGSGGIEVEGLKDVAFALAPLNQAEARKMIRETW
                     AGRKLKGFRSISAVDEESVIDVLIKLSQLAMEQETVSEIEINPLRVLPKGAVAVDVRV
                     KLRA"
     gene            8076..8873
                     /locus_tag="PL000_07760"
     CDS             8076..8873
                     /locus_tag="PL000_07760"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC88832.1"
                     /translation="MIQLSNLTYYPIKACRGFDVAESQVERMGLANDRRMMVVTPSGK
                     FLTQREYPRLALVTPTVKNGMVTLSAPNFDSLQFGIQSTGTPTPVEVWKSKDVSAIDQ
                     GEESAQWLSDWLGVSVRLVHVADGFKRKLNPEYAVHADDHTGFADGYPILIISEESLQ
                     DLNSKLDSAVPMNRFRPNIVVKGCEPFAEETWKRIRIGGVEMALVKPCARCVVTTIDK
                     DTLAKSKEPLKTLSSYRMQELGAIFGVNVIPLNEGVVKVGMSVEVLE"
     gene            8910..9167
                     /locus_tag="PL000_07765"
     CDS             8910..9167
                     /locus_tag="PL000_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88833.1"
                     /translation="MEKTIIDILGWAGTILYLVAYGLISAKKVEGDSWLYQGLNIVAG
                     TFLIINTFYLRAYPSAGLNIAWVGIAIATLGRKAWTKASHD"
     gene            9160..9939
                     /locus_tag="PL000_07770"
     CDS             9160..9939
                     /locus_tag="PL000_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC88834.1"
                     /translation="MTSISRRDFLKLGGAALLGISAGKFIPPQEDEPNFTAPLIWSGS
                     RKFKRMAFTYDDCYLLNKMQDLEELLNEFPDFKVTFFPVGSKLLNLEEQDSGIWKRLV
                     EKGHEIGYHTFDHVNLGVMSPATALMDYDKWHAALNQVLGMEYTVRFVRPPYDIISYT
                     MDVLCQERGLVAAMFSIGGGGEPDIVTRAIQKGKGGDIVQMHIRTQDYKSSVQVFPWL
                     KENSWELVTMSKLYDDYLREQINPEGCELDAGSSLTRVCVE"
     gene            complement(10161..11144)
                     /locus_tag="PL000_07775"
     CDS             complement(10161..11144)
                     /locus_tag="PL000_07775"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNC88835.1"
                     /translation="MKTIPRLNFAHLPTPIEELPHLTAQLGGPRILVKRDDQTGLAFG
                     GNKTRKLEFLVAEAQEQGADLLISAGAIQSNHCRQTAAAAARFGFDCTLVLTGERPAQ
                     SSANFLLDQLFGAEIITVPDRKDRDRILQETFDAALAAGRKPYLVPYGGSSVTGAMAY
                     TFAVQEFMEQNRKVDWIVFGTSSGGTHAGFVLGQRLFGFKGRILGISIDEPEAELKAH
                     VSALASDASEKLGERINFSHDDVLATENYCQAGYGVFGEGEREAIKLFASAEGLLLDP
                     VYTGRAAAGMIDLIRRGFFKKDETILFWHTGGQPALFADKYSQDVVGKLPT"
     gene            complement(11189..11566)
                     /locus_tag="PL000_07780"
     CDS             complement(11189..11566)
                     /locus_tag="PL000_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNC88836.1"
                     /translation="MTETKHNDEVLAIIKAATEVHRELGSGFLEAAYQEAMEIEADLM
                     GIPFVPQQNLQVQYKQHRLKKKYVADMICFDKIVVEFKVLDKLTDKEESQMVHYLKTS
                     GYKTGVLINFGSHPNLEWKQFAD"
     gene            complement(11599..12762)
                     /locus_tag="PL000_07785"
     CDS             complement(11599..12762)
                     /locus_tag="PL000_07785"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNC88837.1"
                     /translation="MNSSLWNQIISALPNPHFLQTYEWGQVKAKYGWVPFYAVWTADG
                     KWKAESDPQALSTLHSPVVAACLILKRTILNRGFAARLSVLYAPKGPLMDWGNASLRA
                     RVLDDLQSFAKKQGAVFLKIDPDVVLGRGIPHSEGDVLDNGGQMVMSELTRRGWGISS
                     EQIQFQNTVLIDLSASEDEMLMRMKPKTRYNVRLAEKKGVTVRTGKLDELGMLYRMYA
                     ETSVRDGFVIRDENYYLTVWKLFMQDRLPDFSLQPVAFPLVAEVDRVPVAAIFLFMFA
                     GRAYYVYGMSRNLHREKMPTYLLQWEAMKRAKAAGCSVYDLWGAPEVFDESDSMWGVY
                     RFKEGLGGEVVRTLGAYDFAPNKLWYKLYADIMPRVLDVMRSRGKEKTKQGLE"
     gene            complement(12762..13754)
                     /locus_tag="PL000_07790"
     CDS             complement(12762..13754)
                     /locus_tag="PL000_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HNC88838.1"
                     /translation="MPEVSLSDWNQFVASHPDAHLLQMGQWGELKNEFGWKPVRLILD
                     RETGAQILFRRLPLGLTLGYMPKPVDSGRLMVDSESFWREVDSICKKNHAIFLKIEPD
                     SWEETFQPSNLPTFKRSNHNIQPPRTVTLSIKEDEEEILARMKPKCRYNIRLAEKKGI
                     TVRAWSDISAFHEMMTVTGGRDNFGVHSKEYYQRAYELFHPIGTCELLVAEFEGRPLA
                     SLMVFSNAKRAWYVYGASNDQERNRMPTYLLQWEAIRWAKARGCEEYDLWGVPDEQEE
                     TLEANFETRRDGLWGVYRFKRGFGGQLKRAAQALDRVYNPLLYWAYVKYMGNRE"
     gene            complement(13756..15639)
                     /locus_tag="PL000_07795"
     CDS             complement(13756..15639)
                     /locus_tag="PL000_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNC88839.1"
                     /translation="MSSRPHIRNRFVLIGDLALIVISVLGSFALRLDVSELPFYFPAA
                     LVMCAVALLVKVPVYFYFGLYRRLWVYASTSELRLITIAVTSASALTSGVMILLSSIG
                     FIQPGMPRSALGIDWLFSLVLIGGSRFALRILAEQSSAPRDALRVGKRALIIGAGDAG
                     ALVVRELQKSSQLNLFPVGFLDDDPAKQNHQIYGVSVIGRVGKLADVLESQRIDEVII
                     AIPSAPGKIIRAVNDACRKKGIPSRTMPGIYELLGGKVSVNRLREVDITDLLRRDHVR
                     VNDEQVGQALEGKRVLVTGAGGSIGRELCRQIARRNPSQLILLGHGENSIFEILLELQ
                     NDYAALKLIPVIADIRNGQRLDSVFAQHHPQVVFHAAAHKHVPLMEANPVEAITNNVL
                     GTRNVVQAALDHNVERFILISTDKAVNPSSIYGATKRLAEAIVLDAAHKNKRAFAVVR
                     FGNVLGSRGSIIPIFKNQIANGGPVTITHPDMFRFFMTIPEAVYLVLQASSMNNGGEV
                     FVLNMGEPVRILDLAEDLIKLSGLEPQKDIEIAFTGIRPGEKLTEELWVEGTSLIQTP
                     HPDIFRLDQDAASALNLDLVQAIEQLSTLSLTTDAQSIARLLDQLIPGSTIFNAPHDY
                     LEV"
     gene            complement(15722..16648)
                     /locus_tag="PL000_07800"
     CDS             complement(15722..16648)
                     /locus_tag="PL000_07800"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC88840.1"
                     /translation="MNYLVTGGAGFIGSHIVQALLKQGDKVRVFDNFSSGKRENLKGL
                     DVEVIEGDLRDASRVAEAVRGMDIVFHEAAFVSVPESMEKPQECFDVNVTGTSILFEA
                     ARKAGVRRAVIASSAAVYGDSTAMPLVEDTPLRQLSPYATSKRVDEKYAQLFTEFFGF
                     DVVALRYFNVYGPRQRPDSMYAAAVPIFIRRILDNKPITIFGDGGQSRDLVNVRDVVQ
                     ANLLASQHPAAPGQIFNVCTGVETRLLDLLDILYELFPNAPRHVHAEPRAGDIYRSIG
                     TPKKIMDTLGFKPQVTLADGLHEAVEEMRGQK"
     gene            complement(16652..18118)
                     /locus_tag="PL000_07805"
     CDS             complement(16652..18118)
                     /locus_tag="PL000_07805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC88841.1"
                     /translation="MADIIKPPLRLRPSEQRTILLAGDLIASVGAMFAALFTWYQFSL
                     VREVARLVERGFSEARAERLAASIIELKIPVWFYILPLVWLLLMVDSYEPHTSASWRK
                     TLRGIAVAPIVGLLGYSLLFTFNQDPESLPRIGVGAFLIFASILTLAWRAIYIRLYTS
                     SGLMRRVLIVGAGKAGHSLADIYRKFSPPPFLLIGYIDDDFRKQSKSYHGFAVLGTSQ
                     KLLEIIETHRISDIVVAINGEIKGETFQTILDAQEQGVEVTRMPIMYEEMTQRVPVEH
                     LESDWVIRSFVDQVRVSGVYELFKRLMDIVGGLLGLLIFAVIFPFTALAIVLESGFPI
                     FYSQERLGKGGRVFMIYKFRSMVQNSEEDGEARPAIVNDPRVTRVGNFLRRTRLDEMP
                     QFLSVLTGEMSLVGPRAERPELVAQFQKQIPFYRARLLVKPGLTGWAQINYGYVATVK
                     ETVVKLEYDLYYIKHRTLNMDFNIVLRTIGTVLRRTGR"
     gene            complement(18111..19121)
                     /locus_tag="PL000_07810"
     CDS             complement(18111..19121)
                     /locus_tag="PL000_07810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88842.1"
                     /translation="MPPFVSIIVPCYNEEATIRHLLNAVFAQTYPRAQMELIISDGMS
                     TDRTREVVEAFQKEHADLEIRVVNNTARAIPSALNQAIREGRGDVFVRLDAHSMPIPE
                     YVERCVAAHEAGKGENIGGVWEIRPGADTAIAKAISFAAAHPLGVGDAMYRLNASAGA
                     VDTVPFGSFRRALIEKIGLFDETLLANEDYEFNTRVRESGGTVWLDPSIRSVYFSRST
                     LGSLSAQYWRYGFWKLKMLKRYPHTLRWRQALPPVFVSSLIFFIVLSLFFGLAKYALA
                     AQLLLYFFVLGLAGLKLAVEKRNAFLIFGLPLAIATMHLSWGAGFLWSFISGFASART
                     HG"
     gene            complement(19148..20527)
                     /locus_tag="PL000_07815"
     CDS             complement(19148..20527)
                     /locus_tag="PL000_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC88843.1"
                     /translation="MFSSLRSRLWLSYALLIVTALGVVAIILFTYLLTNPLLYRQTLE
                     RLRAVQGVVLDNSRLPNAQPISIAAQKASLTFDVRIVQYSREKQVLSDTYSSKEPVLP
                     FPEKRVFARNVPLVRDENGKAWLYSVEILPDKSYLLVAAPRPKLSISNVFTDDFLPLI
                     FRSGLLALLLSLVVAFFFARWIADPLQKVVAAARAMPSAEIKPVEIGGPHEVQELTRA
                     FNSMVARTQASQRSQRDFVANVSHELKTPLTSIQGFAQAMLDGTADSEEARQKAAQVI
                     YDESGRMHRMVLDLLDLARLDSGTADITMSPVKMSALLNAVAEKFAPQSTRAGVRIQV
                     EAAADLPSITADGDRLAQVFTNLVDNALKFTPSGGSILLQASMLDGEMRVAVTDTGAG
                     IPAEAQKHIFDRFYQADPARKGGAKHGAGLGLAIASEIVQAHGGRISVRSRQGEGATF
                     EVFLPLVRK"
     gene            complement(20554..21234)
                     /locus_tag="PL000_07820"
     CDS             complement(20554..21234)
                     /locus_tag="PL000_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88844.1"
                     /translation="MASELILLVDDEPSIIQLSRMYFERDGFRIQEVGDGEAALEAVT
                     KHHPALIVLDVMLPKLDGFEVCRTLRAKGDQTPIIMLTARDEDIDKILGLELGADDYL
                     TKPFNPRELIARVKAILRRSDGKKQADGKPLHRGDLTIDPVSREVSIASQILDLRTQE
                     FDLLFTLAEQPGRVFTREQLLQLAWGFDFYGQTRTVDVHIAHLRKKLEGSNIKIETVT
                     GVGYKLVV"
     gene            complement(21253..21636)
                     /locus_tag="PL000_07825"
     CDS             complement(21253..21636)
                     /locus_tag="PL000_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC88845.1"
                     /translation="MKKKVIQTEKAPKAIGPYSQAIRTDAFIFTAGQIGIDPTTGELI
                     SGAVEEQTRQALSNLRNVIEAAGSNMDNVVKTTVFLKDMADFPKMNAIYAEFFGENPP
                     ARSTIAVAALPKGGLVEVEAVVLAV"
     gene            complement(21841..22377)
                     /locus_tag="PL000_07830"
     CDS             complement(21841..22377)
                     /locus_tag="PL000_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88846.1"
                     /translation="MKTMFKKTILVAIAAALVLAALPFSSAFAMNGSAEGEVTNEKLE
                     QMWARQLQAYERVGKGFEKTDEFTAKVQERIDKAAANGKDVTALQAALDAYEAAVKNA
                     RPAYESIQGIVSAHQGFDANGKVTDAEKAKATVQAMRTKMQEVKTSMNGTFKALRDAI
                     KAFREANQPAEGTSERGT"
     gene            complement(22540..23286)
                     /locus_tag="PL000_07835"
     CDS             complement(22540..23286)
                     /locus_tag="PL000_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNC88847.1"
                     /translation="MINIFMKSPLPNTLAVIVLLALIITWVTSIVLSMRDVPVETEKG
                     WYANLLPVFSILGIPAVLDLLQTKGVTFVFAAIVFTVLLLNIVVPILKIYGKSSHFLV
                     ADWYKWSVLISTVGGLAVAGYLTFVEATNAPVVCGPSDGCAAVQNSKYAVLFGVLPVG
                     VLGLAGYVGILAGWLVLHFGPDAIKRMAALAVWAMCIFGVLFEIYLTFLEPFVIGSTC
                     MWCISSAVLMIILLLVSTPAAQQAFVINDD"
     gene            complement(23343..25832)
                     /locus_tag="PL000_07840"
     CDS             complement(23343..25832)
                     /locus_tag="PL000_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC88848.1"
                     /translation="MKPTLFRIFGYLIIVSLALAACTPTAVPTEAVEEGDFLGAMSQP
                     YELTGSYTVTNDFVLATYYVENAVALTDMHGFVLRDLEWEIPVDSQVLGFMTFDADAL
                     GGTYELSLPAIPQGEFNDVDNNGAADAGVQIFAVAYSPNVYGGPFAEGDDRSRGWPSY
                     LASVKTDSENKDEVIGGKLVVWAEDANQQFPTSFGDDGLLFTADDPVAPISQGYSIVD
                     LDQVPFAVTQDAISDLPLYEPEDIATKDYSTLSYTEAFDKMFEVIRNEYAFADIPGEA
                     PDWDALYAELKPRVEQAEKTSDPNAFYLALRDLTWAFDDGHVSLNGGQYFQQDFATAV
                     SGGYGFAIRELDDGRVIVIFVMDGGPAQAAGIQVGAEVTEFNGLPISDAISGAQSYSN
                     QSSEFAIRYQKSRYLLRALPGTEATVKFANADGIPQVVTLTAIEERQSFSRTSLYFGV
                     DNSSLLPVDLKIIPVNNDNVGYIRINSNYDDLNLVIRLFERALKQFEANEVAGIIIDM
                     RYNNGGAPLGLAGFFTEQEIPLGQLEYYSDKTGKFEPEGLRQKFLPNQNQYRFEKTVL
                     LVGPACFSACEIEAYGFSQVPGMVVVGLTPTSGVEAETARGTFNLPEGFSVTVPTGRF
                     TLPDGSIFLEGQGVPPTLRIPINEETATAIEDIVLQAGIDAVLQPFGAGVTPSAAPKV
                     ASQTEAESALSSGAPFLEDLARESYDPAITFAQPGTATYSISMTDPEPVIWGYSWCAV
                     DAATVASNFEKIDMQFMLDGKEVPTDSFGTLELETGGKVCRLIYTSLSEWTSGEHHLS
                     TTATFTAAINDGSANFEAGEYILEYTVYVKP"
     gene            25902..26231
                     /locus_tag="PL000_07845"
     CDS             25902..26231
                     /locus_tag="PL000_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88849.1"
                     /translation="MDNVTTQLQKEFEKAQQARIAKNEGQARVCARRAAGVAIRDYLT
                     RKGDKIPSMSSYDLLNLLKDNAALPPDLKLAVDNLTVRVTEEFTLPVDADLIAEARKL
                     CNWLLSN"
     gene            complement(26355..26624)
                     /locus_tag="PL000_07850"
     CDS             complement(26355..26624)
                     /locus_tag="PL000_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88850.1"
                     /translation="MMTRRNTSTTLEDLDTLEAHLAGTLKPISPPKELVQRLGARIRV
                     PSRSEITLRFTDWRRMFFVVGGVVSGMLLLITVARALYYLAGRRM"
     gene            complement(26640..27482)
                     /locus_tag="PL000_07855"
     CDS             complement(26640..27482)
                     /locus_tag="PL000_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC88851.1"
                     /translation="MLRIVTDGAADILPAWGQEYGIEVIPVNILFGEKSYLQGVELDN
                     EGFYKLVDDTKRIPKTSQPSPHQFVEFYRKIAQKGDTILSIHITAKLSGTYASAIAAA
                     EELKDEFKVIPIDSAVGSLGIGMMCREARRLERAGKTVDEIVSYLEDIKYKIRVILTL
                     DTLEYAKMSGRVKTLQAALASLLNVKPIAILRNGDLNMAERVRTRKASLDRVIEIAEE
                     EFGKKPVYLAVVHARDAKSGQGLLDDAKSHFNHKETMLSDLSISIAANLGPGTVGLIL
                     YPAE"
     gene            complement(27486..28049)
                     /locus_tag="PL000_07860"
     CDS             complement(27486..28049)
                     /locus_tag="PL000_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC88852.1"
                     /translation="MEYNEEEILLRASQGDRDSFGQLYAHYVERIFNYVYYRTGNTHD
                     AEDLTARVFQRAMKHIVNYTDRGVPFSAWLYRIAHNLVANWHRDRSRKQEIPLDDLPI
                     LPTKGDHPERNLVRSQEQDVLLKMIRTLPAERQNLLILKFVENMSNAEIGAIMGRSEG
                     AVKSLYHRTLLALRDQLEDQNLNFEGE"
     gene            28225..31101
                     /gene="uvrA"
                     /locus_tag="PL000_07865"
     CDS             28225..31101
                     /gene="uvrA"
                     /locus_tag="PL000_07865"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC88853.1"
                     /translation="MSTNVIRVVGARVHNLKNITVEIPRDKFVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFIGQMDKPDVDYIDGLSPAVSIDQKSTSHNPRSTVGT
                     VTEIYDYMRLLFARAGIPHCPICGREVVKQSAQEIVDKIEKLPEGSRVLILAPLVRAR
                     KGTYQAVFEEIRKAGFTRARVDGNTHSLDEEIELDRYKQHTIEAVVDRLVISQEGDKE
                     EKKSARTRLTDSVETALKFGEGYITVNLVDKKEDIQFSEHLACPEHGVSISEIEPRTF
                     SFNTPQGACPDCQGLGSKLEIDPERIIPDPDRSLNEGAISSMEWSGPKEEGGYYWQGL
                     EAAAHAYKIDLNKPVRELTKDQMKIILYGTGEKQIQMNYKNSNGNEFKFTRAFEGVIT
                     NLERRYRETNSEWIREKISEFMSDRPCPTCNGKRLNPAALAVTVDGVNIVEANSWPVL
                     KTLDWVKKISGKGSPLTSKQKTIAERVIKEIHERLNFLVNVGLDYLTLNRSAATLSGG
                     EAQRIRLATQVGSRLVGVLYVLDEPSIGLHPRDNSRLLETLKGLRDLGNTVLVVEHDD
                     ETIRESDWIIDLGPAAGEHGGEVIAEGTPKQILAHPKSLTGAYLSGRKQIEVPKKRRE
                     GNGKALKIVNATANNLKGINVSIPLGKMVCITGVSGSGKSTLMSDILYNSLAAKLMSA
                     RTSAGEHERIEGIEHLDKIINIDQSPIGRTPRSNPGTYTGLFDEIRKLFAELPESKVR
                     GYKPGRFSFNVHGGRCEACQGQGELRIEMQFLPDVYVPCDVCHGKRFNRETLQVLFHD
                     KYSISDILDMTVDHALDEFKNYPPMQSKLKLLQEVGLGYVRVGQPATTLSGGEAQRVK
                     LSKELSRRATGRTLYVLDEPSVGLHTADVHKLIEVLQRLVDSGNSVVIIEHNLDIIKI
                     ADWLIDLGPEGGDRGGQLLAEGTPEQVMKVKESYTGKYLKAHVK"
     gene            31277..32101
                     /locus_tag="PL000_07870"
     CDS             31277..32101
                     /locus_tag="PL000_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC88854.1"
                     /translation="MNNRPPISSSPNPGTISLLRKRKRNPNIVLIAAGVLVLGGIIML
                     VVWLTGDSSPLNALLATETPTPTMTFTPTSTSTPTSTATATETPTITPTPTFSTIFNY
                     TVEAGDSLFSIVEKFNLGENGVALILLLNPYGGIADSGFPIGVDPATLNILPGQSIVL
                     PYPGMPLPTATPLPANLAPGTKIEYIIQSGDSLGSIAAAFNSTIDEIIAANGITDPNA
                     IKVGDTLQIPVNVVTATATRPPTSTPITPGPGTLLPTVTFTPINALPATATATPTP"
     gene            32193..32477
                     /gene="rpsT"
                     /locus_tag="PL000_07875"
     CDS             32193..32477
                     /gene="rpsT"
                     /locus_tag="PL000_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNC88855.1"
                     /translation="MANINSQIKRNRQNEKRRIHNRNFRGAARTAITAARSSLAKSAP
                     ETQEDIMLAVSALDKAAQKGVIHPNNASRRKGRLMKKLAAFLSAKSEKKA"
     gene            32584..34401
                     /gene="argS"
                     /locus_tag="PL000_07880"
     CDS             32584..34401
                     /gene="argS"
                     /locus_tag="PL000_07880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNC88856.1"
                     /translation="MFTKEQQLIEEKIKAYCQANDIALAELKWQPIPFSGEWGFATSF
                     FQTAANEVKLGKGNKLPVPQKAQEIAEQVRGQIGSVDGISHIEAVKGYLNVYFKTSDY
                     AQRVVDEVLASGLNFGRGAAKNERVMVEYAQPNTHHSFHIGHARNTILGEVLARLVEF
                     AGFDTIRASYPGDLGLGVITVMWIYEKFYKGQEPTGIHERGQWLAKLYVEATALLEKK
                     ENETPEQTAQREAYEAERREMYRKWDAGDEAVRKLWHETREWSLEELRDVLRMLDVKM
                     DVWFYESEVDEPSKAIVEELIAKNIADDERPQGGAVIVKIDEKLGLTKEKYRTNVILR
                     RDGTTLYLTKDLALAKVKFEQYHVDRSIYVVDVRQSLHLQQAFAILKLWGFPQSEKCY
                     HLGYGFVSLPEGAMSARRGRVVLFKDVADEAVKRVLSVETEKSADIPAEEREKVAYQI
                     GMGALVYSMLSVDNNKDIVFDINEALSFDGRTGPYIQNAHVRANSILKKAGVSTFERS
                     NVQTFNYDFTKHEIELIEQISRFPKAVQQAAEEYRPLVMASYAYELANTFHSFYHAVN
                     VMQTEDENTKNARLRLVAAAKQTIANALRLLDIQAPDVM"
     gene            complement(34449..>34679)
                     /locus_tag="PL000_07885"
     CDS             complement(34449..>34679)
                     /locus_tag="PL000_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88857.1"
                     /translation="CPSIVAEVGMTIAWENVDTVSLPIKIEFLDENGKVTDTGLSVID
                     PETVFSTSFPEAGVYYVYCTENADIPSTITVK"
BASE COUNT         8851 a         9436 c         8708 g         7684 t
ORIGIN      
        1 gaaggtctgc ccgtgtggga atggcttcag caatttattg acccaatgca tcgcgtggtc
       61 aacgagcgtg aagcggagat cgcatcgttc ctgtgctatg ccgaatcttt gctgtgcggc
      121 acgaccaccg tcgtggacat gtggcgtcac atgcatggca gcgcgaacgc cgcagcgcag
      181 ttgggaatcc gcgcggtgct ggtgccgtat gtggcggagc atcccgacca cgattatttt
      241 gaaacgctca agaccaacga agcgctcatc caaaaatggc acggcggcgc ggatggtcgc
      301 gtgcaggtct gggtcggctt ggagcacatg ttctacgcca cgcccgaagc ctgggggcga
      361 gtcaccgcga tggcgaagga atatcaaacg ggctttcaca cccacagcaa cgaaagcaaa
      421 ttcgatgtgg aagaaaccgc gcgtcgttac aacatccgtc ccatcgaagc gcttgaaaag
      481 ttcggactgc tcgatgcccc caaaaccctg ctcgcgcatt gcgtctggct gaacgattct
      541 gaaattgaca ttctcgcggc gcgcagaatc ggcgtggcac acaaccccat cagcaacatg
      601 aaactcgcca gcggcgcggc gcgtgtggtg gacatgctca acaaaggcgt cgcggtcggt
      661 ctcggcaccg acggcgaaaa agaaaacaac aacctcgaca tgttcgaaga actcaagacc
      721 gcctccctgc tggcaaaact ctccgcgctc gatgccgccg cactggacgc atggtccacc
      781 tgccgcatgg gaacggtcac cggcgcacgt gccatcggtc tggattcgga gatcggctcg
      841 atcgagatcg gcaaacaagc ggatttgatc gccgtccgca ccgacacacc gcgcctcacg
      901 cctctcctct cagggacggc tggcaacctg caccacaaca ttgtccacgc tgtcagaggc
      961 ggcgacgtgg acatgaccat ggtcgcaggc aacatcctcg tggacggagg tcaactgctc
     1021 acagcagaca tccgtcaact gattgacgaa gccaacaccg ccgtgcctcc gctcttcaaa
     1081 cgccgcgccg aatggctggc ttcgcacaaa tcggtcaatg aactggtcaa acacaaggac
     1141 gactagcacg gtccgcactt gaaagcgtgt cctggccttg ctacaatgaa tttggaaaag
     1201 atcgcgaggt aaaaatgatc tccatgattc ttggcacgct tacggtcatt gccctgttgc
     1261 tggttgtggg agccctgatc cacctcaaat ggacggaaac catttcagag agaaccatat
     1321 cgcgcaggat caaggcagag taccccgccg aggtccaggc gcaggtttac cagacctatc
     1381 aacacatgaa ggtgaaggaa ctcgaagggc tcttcctcaa aattctcgat gatgcaaatg
     1441 gcgacgtatt gaaagttcaa aaactggcca gcatcgccga gagcgtgagt tggcgcgcct
     1501 tcctcgagaa tcactggtaa agccgcatag tacctgcgtt tgatatgcac gcccagccgt
     1561 atggctataa tccgatcaaa atcttttgat ggaggcatat catggcaact ttggaacaag
     1621 aggagatcgc cctccttaaa gggcgcatca gcagactgga agcccgcctg gaatttctct
     1681 acaagcattt gaatgttacg tttgtggaag atacccgcct gagcgacgac ccgaaagtga
     1741 tcgcagcgct aaaggcaaac aacatgctcg aagccatcaa attgtaccgg gccgcgacga
     1801 atacaagcct tgaagccgca aaagccggtg tggaggaaat gcgggcaagg ctgggtattt
     1861 aacaaaccgg taaaactatt ttcaacagaa acagggatgc gggcatcgca tccctgtttc
     1921 tgttaaatca acttacagat tagtaaaccg gtgaaatcga agtccctttc acacccatct
     1981 atggtttaat cgcccgaaac acaaaaagaa ttttacgcaa acagggagag caggatgcca
     2041 aacatagccg cttttgttcg caaggtctat tttccattgg tcagtttgct ttgtttgatc
     2101 ggggtgatcg gaacgggggc actttcctgg cgcaccagcc aggatcaaga tcagcgccag
     2161 caggcatctt ttgaatttgg cagcgatcaa gctgaacggg tcgcaaccga ggtcaacgca
     2221 gaaatgaacc tgcaaatgac catcgccgaa acgatcgcag gtgaaatttc cgatggcaca
     2281 ctggcctaca cggagatcac cggccgcctg cgcgatgaac tggatgcgca tcccaatttt
     2341 tttggaatgg gtgttgcctt tctcccgtat gtatataaat cagacctgaa attatttgcg
     2401 ccctactatt tcaaaaacga gttcgggaac ttcacccgcg cccaggtgga aacagactac
     2461 gattacagtg acagagccac acaggataac gaatggttct atagcgccct tgaagacggt
     2521 gcctattggt acgccagcga atacgatatc accgcccagg ctgagttggt cgaatacatt
     2581 accccttttt acagaaccaa cccgcaaaca ggtcagcagg aagccgttgg ggtcgtttgg
     2641 gtggatcact ccgtagagac gatgaagagc tttatccgct cgatagacgt cggcgaagat
     2701 gggtacacca ccctgatcga aagcgccggg tacatcgttg tccaccccaa ggcagaatac
     2761 atcaacaaga ccctgtatga agtggcggaa ctggtgggcg ataccgagtt aagcgcagcc
     2821 gcagaccgcg ccatcagtgg cgaagatttt tacctgcagc gcaaacccac cgtcaacttg
     2881 aacagcaccg catggacatt cttcaaacca ctgatagccg aaggctggtc gatcgcggtc
     2941 accatcagcg acaatgtgtt tgaagccccg ccggaagaac ttctgcgcac acagatatgg
     3001 ttaagcatta tgattatgac tgccctcatt tctctggcgt tgctgatctt tcgagtggat
     3061 gaaggtgcag tgcgtaatct ctggatcgtc gttggctgga gcaccttcgt tcttctcagc
     3121 ggcgtcatgt ggatgtggca aatcgtcaac acgaatccct ccaacattac cgcatcaaaa
     3181 aatatcctgg tcagcacctc ggttgtgaat gaaagcctga aatccgtgga cgagaccgca
     3241 acaataaacg gccgccccat tgccctgcgc atccccaccg gggtcctcgt gcagaatata
     3301 cagatccagc ccaacgaagc tgtgatctcg gggtacctgt ggcaaaaata ccccaaggaa
     3361 ctcccgccca atttcatgga acatctggag catgaattca ttcaaggcgt tgaatttccc
     3421 gacgacacag gggcaacggc atttgaagaa agttatcgct tccaacgcgc cgattccgag
     3481 atcgtcggct ggttcttcac caccacactg catcaatcct acaacacggc ccaatatccg
     3541 ctcgatcagg ccaatattca gatccgcata tcaccgcagt atctcgacca gaacatcgta
     3601 ctggtcccag atttcgaaag ctacgatttc gtcgccccct cgtccctgcc cggtctttct
     3661 ccccagatca acattccaga ttggaacatc caacgtagtt tcttcagcta tcaacttcag
     3721 cagttcaaca caaattttgg cagtcaaacc cacatccaaa gaaacaacct gcccacactc
     3781 accttcaaca tcagtgccag cagaaacctg ctctccccca tcattgcatt ctgcatcacg
     3841 atcatcgtgg tggcggggct tatgtttgga agcctcatcg tcaaactcga ttcagcgtac
     3901 agcgccctct ccaacgcagc cgcgctcttt ttcgtggtag ccatcacaca cgtggggctg
     3961 cgcggttcca tcgctgctgg cggcacggtt taccttgaat actttttcat cgtgctttac
     4021 attttcatcc tcggcctcgc cctcgatggt ttaatgtatt acaacgaaag cgccattccc
     4081 atcatcagct atggcgaaaa cctcattcca aaactttgct ttgggcccgg gcttgccatc
     4141 acgtttttag cgatcaccat ctttgtcttt tatccgcgca cggtcgccgc agacccacta
     4201 caggaatatc tctccatcaa cgcgcttcaa gcgactgaag aacccgccgc atcggacctg
     4261 acgccaaccg cctcccccgt aacggacagt ggatcagccg cgaataacct gacagccacc
     4321 ccaatcaggc cgtccacctc cccagccgcc accaccgttc ccgtcatcct gcccgaagac
     4381 ccgatcaccc ttcgttaccc ctttggcctc gaacctccca cacttgaccc ttcccttgtc
     4441 agcctcacct cagaccagga tcaggttgag cagctcttca tcggcctcac ccgtcttgag
     4501 tctggcacca ataccccgct tcccaacctc gccgccagtt ggacaacctc cgaagacggc
     4561 ctcacatgga ccttccacct tagaaacgac atcccctggg tgcaatataa cgccgccaca
     4621 aacagcgtct cacaggtgac cgatttcaac ggcacgccac gctttgtcaa tgcatatgac
     4681 gtggtctacg gcattcacca tgccctcacc tccgaaaata catcggcggc tctgttatat
     4741 gagatcaaaa acgccgaaca acccgatgac gacctcggcg taaaagccct cgacgagtgg
     4801 acattagaga tcaccctcaa cagacccgcc ccttattttg ccagcatcgt cgcctatgac
     4861 gtcagctacc ccgtccctgg ctgggccatt gaagaatggg ataaggaatg gatcgccccc
     4921 gagaacatcg tcaccaacgg ccccttcgtg ctggcttcat gggaaaaggg aaaaggcatc
     4981 accctgctca agaatcccct ctggccagaa gcggataaaa tccagatcga acgcgtggaa
     5041 gagattctgg ttcaggatgg agaaattgcg ctaaaactgt atcgcgacaa tcaaatcgac
     5101 accctcggcg gcttgcggag caacatcctt caggaagtga aaagtgaccc aacacttgcc
     5161 aatgaattaa gcagccattc caacctgtgt gtacgttatt tcggttttac gaatacgaaa
     5221 ccgcccttcg acgacccacg tgtgcggcgt gcattctccg ctgctgtgaa ccgacaggag
     5281 atcgtggaga tgctcaaagt gggcaacatc cccgctttca cctttgcacc tccaggaatt
     5341 ttcggggccg taacagaccc aggcgttggg cagggattcg atgcagacct cgccaaacaa
     5401 ctcttccagg aatatcttga cgagcgcgga tttacgattg ccgatttcaa ctctgcgtac
     5461 ccgcttgtga tgggacaact gaacgggctc aacaactccg cctccgatat agccatcaag
     5521 aattggaacg acatcctcgg cgtgaccgtt acgaccaaga cagtggaaac cgcagagttt
     5581 cagagcagca ttgccaaaga cacccccatc aaaaatgcgt tcagcatgtt ccaatgggca
     5641 tggtgcgccg actaccccga ccagaacgat ttccttcacg tcctctttaa ttctgaaact
     5701 ggcacgaacc tcgtgcgccg caattgcgcc gatgaaacct gcaccgccat tctgccaaat
     5761 gatgagttcg acaacctgac attgatggcc tcccagatca atgaccccga agagcgccag
     5821 aggctttacg cacaagccga gcacattctg gttgtggaag aggctgcact ggcaccgatc
     5881 tattacgggg catccaccag cctcaccaag ccgtggctga cgcgcaactt ccccaacatg
     5941 ggtgcgccag atttcatgaa ctggatcata gatattttgg cccaaaagac aacaccatga
     6001 gcatggtata accttccaat ggataaatcc ctcactccct ttttcaatcc gcgcggcgtt
     6061 gtcgtcgttg gtgcatccac gtcacctgaa aaattggggt atggggtcgc gcggaatttg
     6121 atcgaaagca aatatcgggg cgcgatccat ttcgtcagcc agaaaaaagg cgaattgttc
     6181 gggcacccgc tgcttctgag tctgaacgag gttcccgatc ctgttgacct tgcgatcctc
     6241 attgtcccga cccaatccac accgcaaacg atcgaagact gtgccaggcg cgggatcaag
     6301 gctgccatca ttgtgtcatc tggttttcgc gaagtcggtg cagaaggtgc tgcgctcgaa
     6361 gcgcaatgtg tggagcttgc ccgcaaacac ggcgtgcgcc tgctcggccc caactgcatt
     6421 ggcaccctcg atacacacct gccattggat acaacttttt tgcagccgcc catgcccgtg
     6481 aagggcggca ttgctttcgt ttcgcattcg ggcgcgtttt gcgccgccat cattgactgg
     6541 gcgcgtgaac agggcttcgg cttttcgcag atcgtcagcc tgggcaatca ggccgatgtc
     6601 aacgagaccg atgttctccc tgcagttgcc aatgatgagc acaccaaggt catcgcgttg
     6661 tacatggaaa gcgtttcaaa cggggtgaaa ttcgtgcaag ccgcaagcga agtcaccagg
     6721 cacaaacctg tcattgcgtt gaaggtcggc cgcttcgaag cgggtcaaaa agctgctgcc
     6781 tcacacacgg gcgcactggc tggctccgaa gcagccttcg atgctgcctt tgccaaagca
     6841 ggcgtcctcc gcgccgacac cgccgagcag atgttcgact gggcacaggc cttggaaaat
     6901 tgccccctgc cgcgcgggaa acgcatggcg atattaacca acgcaggtgg accaggcgtc
     6961 atcgccgccg attctctcga aacgaacggc ctgctcctgt ctcaattaac agagtccacg
     7021 ctgaaggctt tgacgagctc cctgcccccc tctgccagtg tccacaaccc tgtggatatg
     7081 ctcgcctctg cctcacccga tgcctacgcc acctgcttga atcttctcct gcaagatgaa
     7141 accgtagacg gcgtgctcgt catcctgccc ccgccgccga tgtataaaac cgaagaagtg
     7201 gcgaaaaaaa ttattagggt aattggcaat ttcgaaaaac ctgttgtcgt tgccttgatg
     7261 ggttcgaccc tggtggagaa tgccagaaaa gaattccacg cggcaagtgt cccgaactat
     7321 ccatttcccg agcgcgcggc atcggctctg ggagcgctgg caaaacgggc agagtttttc
     7381 accacagagt cacagaggca cagagaatta aaaaccccgc gtctcggcgc ctccgtggtt
     7441 aatttatctg ctgatgaatt agtctctgcc tacggcattc caaccacgcc gatcaaactg
     7501 gcgcggaacg aaaacgaagc cgccgccatc gcagacgaac ttggcttccc cgtggtgatg
     7561 aagatcgcct cgcccgatat tttgcacaaa tccgatgtcg gcggagtttt actcaacctc
     7621 acaacccctc aaacgttatt aagtgcctat acgcaaatga tggagcgaat taaatccgca
     7681 atgccagcgg cgaaaattga cggggttcat ttgcagcggc agatccccga agggcaggag
     7741 gtcatcatcg gcatggtgcg cgatccgctg tttgggccgc tcatgatgtt cggttcaggc
     7801 ggcatcgaag tggaaggtct caaagatgtg gcgtttgctt tagcaccgct gaatcaggcc
     7861 gaggcgcgga aaatgatccg cgagacttgg gcaggtagaa agctgaaagg ctttcgcagc
     7921 atatcggctg ttgacgaaga gtctgtcatc gatgtcctca tcaaactttc acagcttgcg
     7981 atggagcagg aaaccgtgag cgagatcgag atcaacccgt tgcgggtgct gcccaaaggc
     8041 gcggtggctg tggatgttcg agtaaaattg cgcgcatgat ccaactctcc aatctcacgt
     8101 attaccccat caaagcctgc cgcggatttg acgttgccga atcgcaggtg gaacgcatgg
     8161 ggcttgccaa cgaccgccgc atgatggtgg tcaccccctc tggaaagttt ctcacccaac
     8221 gcgagtatcc gcgcctggca ctggtgacac ccaccgtaaa aaacggcatg gtcacacttt
     8281 ctgcaccgaa ttttgattcg ctgcagttcg ggattcaaag cacaggcaca ccgacccctg
     8341 ttgaggtttg gaaaagcaag gatgtcagtg ccatcgatca gggcgaagaa tctgcacagt
     8401 ggctttcaga ttggctcggt gtttcggtgc ggctcgttca cgtggctgac ggcttcaaaa
     8461 gaaaattgaa tccagaatat gccgtccacg ccgatgacca tactggattt gccgacggct
     8521 acccgatcct gatcatctcc gaagaatcgc ttcaagactt aaactcgaaa cttgactcgg
     8581 cggtcccgat gaatcgcttc cgcccgaaca ttgtggtcaa aggctgtgaa ccgtttgcag
     8641 aagagacctg gaagcgcatt cgcatcggcg gcgtggaaat ggcgcttgtg aaaccctgtg
     8701 cccgctgtgt cgtgacaacc atcgacaaag acacccttgc caaaagcaag gaaccgctca
     8761 agaccctgag ttcctatcga atgcaggagt tgggcgccat cttcggcgta aatgtcattc
     8821 cgctgaacga aggtgtggtc aaggttggca tgagcgtgga agttttggaa tagcagaaga
     8881 acgcgttaca ccacgtcaaa tgagtaacca tggaaaaaac aattattgat atcctcggct
     8941 gggctgggac aattttatat ctcgttgcct acgggttgat ctctgcaaaa aaagtggagg
     9001 gtgattcgtg gttgtatcag ggcttgaata tcgttgcggg cacgtttctg atcattaata
     9061 ctttctatct gcgtgcctac ccatcggcag gcttgaacat cgcctgggtg ggaatcgcaa
     9121 ttgccacatt gggccgcaaa gcttggacaa aggcttcgca tgactagcat ttcacgcaga
     9181 gactttttga aactgggcgg agcggcattg ctcggcattt cggcgggcaa attcatcccg
     9241 ccacaggaag acgaacccaa tttcaccgcg ccgcttattt ggagcggcag ccgcaaattt
     9301 aagcgcatgg cgttcacata cgacgattgc tatctgctga acaaaatgca ggaccttgaa
     9361 gagttgttga acgaattccc cgatttcaaa gtgaccttct ttcccgtcgg gtcgaaactg
     9421 ctcaatttgg aagaacagga ttcaggtatc tggaagcgtc tggtcgaaaa gggacacgag
     9481 atcggttatc acacctttga ccatgtcaac ctcggcgtca tgtcacctgc cactgcgttg
     9541 atggattacg acaaatggca tgcagccttg aatcaggtgc tgggcatgga atacaccgtg
     9601 cgattcgtgc gcccgcctta cgacatcatc agctacacca tggatgtact ctgccaggaa
     9661 cgcggattgg tggcggcgat gttctccatc ggcggcggcg gcgaacctga catcgtgacc
     9721 cgtgcgattc aaaaaggcaa gggcggcgac attgtgcaaa tgcacatccg tacgcaggat
     9781 tacaaatcaa gcgtacaagt gtttccgtgg ttgaaagaaa acagttggga gttggtgacc
     9841 atgagcaaac tctacgacga ttacctgcgc gagcagatca accccgaagg ctgtgaatta
     9901 gacgctggaa gttcattgac gcgggtgtgt gtggaataat atatagagaa tagtcagaag
     9961 taaaaaagag gcagccgctt tctacagcgg ctgcctttac catttctcta ttttccaact
    10021 agccacccga cagtttgttt ttttgacccg tgcaacaaga aaaagactaa ccactgaggc
    10081 acggagacac agagaaaacc atcaaaaaac tccgtgcctc ggtgtctccg tggttcaaat
    10141 gtgttcatta actaaaactg tcaggtaggt agttttccaa caacgtcttg agaatattta
    10201 tccgcaaaca gtgcgggctg gccgcctgtg tgccagaaca ggatggtttc atcctttttg
    10261 aaaaatcccc ttcggatcaa gtcgatcatt ccagcggcgg cacggcctgt gtagacgggg
    10321 tccaacagca gaccttcagc gctggcaaat aatttgatcg cctcacgctc cccttctcca
    10381 aaaacaccat accccgcctg gcaataattt tcagtcgcca acacatcgtc atggctgaaa
    10441 ttgatccgct caccgagttt ctcactggca tctgaagcca gcgcagagac gtgtgcctta
    10501 agttccgcct ctggctcatc aatgctgatc cccagaattc gccccttgaa gccaaacagc
    10561 ctttggccca gcacaaaccc cgcatgggtt ccgcctgaag aagtgccgaa gacgatccaa
    10621 tccactttgc ggttttgctc catgaattcc tgcacggcga aggtataggc catcgcccca
    10681 gtgacactcg acccgccata gggaacaagg tagggtttac ggcccgctgc aagggctgca
    10741 tcgaacgttt cctgtaaaat gcggtcacgg tccttgcggt ctggcacagt gatgatctcc
    10801 gcaccaaaaa gttgatccaa aagaaaatta gcagaagact gcgctggccg ttcacctgtc
    10861 agcaccaagg tacagtcaaa cccgaaacgg gccgccgctg cagcggtttg gcggcaatgg
    10921 tttgattgta ttgccccagc cgaaatcagc agatcggctc cctgctcctg tgcctccgca
    10981 acgaggaatt ccaatttgcg ggttttgttt ccgccaaaag ccagcccggt ctggtcatcg
    11041 cgcttgacca aaatccgcgg tccgccaagt tgtgcggtta aatgaggaag ttcttcgatc
    11101 ggtgtgggaa ggtgggcaaa attcaatcgg ggaatggttt tcattggtct cctttatgat
    11161 ccataaaaac acaaaactgc atgaaagttt aatctgcaaa ttgtttccat tcgagatttg
    11221 gatgactgcc gaagttgatc aacacacccg ttttataccc agaagtcttc aggtagtgga
    11281 ccatctgaga ttcttcttta tcggtcaatt tatccagaac tttgaattcg acaacgattt
    11341 tgtcaaagca gatcatatct gcaacgtatt tctttttcag gcgatgctgt ttatattgaa
    11401 cctgcaggtt ttgctgcggc acaaaaggaa tacccataag gtcagcttcg atctccatcg
    11461 cctcctgata cgctgcctcc aaaaaccctg agcccaactc gcgatgtact tccgtggctg
    11521 ccttgatgat cgcaagtact tcgtcgttgt gttttgtctc agtcattttg cctccaaaaa
    11581 attaacgtcc agccgattct attccagccc ctgtttcgtt ttctcttttc ccctggaacg
    11641 catcacatcc aacacgcgcg gcatgatatc ggcatagagt ttgtaccaaa gtttatttgg
    11701 ggcaaaatca tatgcgccaa gcgtccgcac cacttcaccg ccaaggcctt ctttgaagcg
    11761 atacaccccc cacattgaat cactttcgtc aaacacctcg ggcgcacccc acaggtcgta
    11821 cacagaacaa cctgccgcct ttgcgcgttt catggcttcc cattgtaaaa gataggtcgg
    11881 cattttctca cggtgcaaat tgcgcgacat accatataca taatacgccc gccccgcaaa
    11941 cataaacagg aagatcgccg caacgggaac acggtcaact tcggcaacga gggggaaggc
    12001 aacaggttga agactgaagt ctgggagtcg gtcctgcata aacagtttcc agaccgtcag
    12061 gtaataattt tcgtcacgga tcacaaagcc atcgcgcaca ctggtttcgg catacatgcg
    12121 gtagagcatc cccaactcat ccagcttgcc agtccgcact gtaacgcctt ttttctctgc
    12181 gaggcgcacg ttgtagcggg tcttcggttt catgcgcatg agcatttcat cttccgatgc
    12241 ggatagatcg atgagaacgg tattttggaa ttggatctgc tccgacgaaa ttccccatcc
    12301 cctgcgcgtc aattcggaca tcaccatctg gccgccgtta tcgaggacat ccccttcgct
    12361 gtgagggatt ccgcgtccaa ggacaacatc agggtcgatt ttcagaaaga ccgcaccctg
    12421 cttcttcgca aaggattgca agtcatccaa cacacgggca cgcagggatg cattgcccca
    12481 atccatcagc ggacccttcg gcgcgtacaa cacagatagc cgtgcggcga atccgcggtt
    12541 gaggatggtg cgtttgagaa tgagacaggc ggctacaact ggtgagtgta aagtggaaag
    12601 cgcttgcgga tcgctctccg ctttccattt tccatccgct gtccaaacag cgtaaaacgg
    12661 cacccatcca tacttcgcct ttacctgccc ccattcatag gtttggagaa aatgcgggtt
    12721 gggaagtgcg gaaatgattt gattccaaag actactattc atcactctct atttcccatg
    12781 tacttcacat acgcccaata caacagcgga ttgtacactc tgtccagggc ttgtgcggcg
    12841 cgtttgagtt gaccgccgaa tccacgcttg aaacggtaaa cgccccaaag cccgtcacgg
    12901 cgggtttcga aatttgcctc gagggtttct tcttgttcgt cagggacacc ccacaggtca
    12961 tattcttcac agccgcgcgc ttttgcccag cgaatggctt cccattgcaa aagataagtg
    13021 ggcatgcggt tgcgttcctg atcgttggaa gcgccataga cataccaggc acgttttgca
    13081 ttggagaaga ccatcaggga ggccagtggt cgtccctcga attctgcgac gagcaactca
    13141 caagtcccta tggggtggaa caactcatag gctcgttgat aatattcctt tgaatgtacg
    13201 ccgaagttat cccgtccgcc tgtcacggtc atcatttcat ggaaggcgga gatgtcactc
    13261 catgcgcgga cagtgatgcc tttcttttcc gcgaggcgga tgttatagcg gcacttcggc
    13321 ttcatccgtg caaggatctc ttcctcatct tctttgatgg aaagcgtgac ggtgcgtggg
    13381 ggttggatgt tatggtttga acgtttgaaa gttggaaggt tggaaggttg gaaggtttct
    13441 tcccatgaat cgggttcgat tttcaggaag atggcgtgat tctttttgca gatcgaatct
    13501 acttcacgcc aaaaagattc gctgtcaacc atcaaccgtc cactgtcaac tggtttcggc
    13561 atgtagccga gagtcaagcc caagggcagg cggcggaaga ggatttgggc acccgtttca
    13621 cggtcaagta tgaggcggac gggtttccat ccaaactcat ttttgagttc accccattgg
    13681 cccatctgca acagatgcgc gtcaggatga ctggcgacaa attgattcca atcggaaaga
    13741 gagacttcag gcatgtcaga cttcgaggta gtcgtgtggg gcgttgaaaa tggtggaccc
    13801 agggatcagt tgatcgagaa gcctcgcaat ggactgggca tcggtggtga ggctgagggt
    13861 ggataattgc tcgatggcct gaaccagatc aaggttaaga gcagatgcgg catcttgatc
    13921 gagtcgaaaa atatcggggt gcggagtttg aatgagagaa gtgccttcaa cccaaagttc
    13981 ttcggtcagt ttttcgccag ggcggatgcc tgtaaaggca atttcgatat ccttttgcgg
    14041 ttcgaggcct gaaagtttga tgaggtcttc ggcgagatcg agaatgcgga cgggttcacc
    14101 catgttgagg acgaagactt cgccgccgtt gttcatggac gaagcctgta atacaagata
    14161 cacggcttca ggtatggtca tgaaaaaacg gaacatatca gggtgtgtaa tggtaacggg
    14221 gccgccattg gcgatctggt ttttgaaaat agggatgatg ctgccacggc tgccgagcac
    14281 attcccaaag cggacaacgg cgaaggcacg tttgttcttg tgtgcagcat cgaggacgat
    14341 cgcttcagca aggcgtttgg tggcgccata aatactggag ggattcactg ccttgtcggt
    14401 ggagatgagg ataaatcgct caacattgtg atcaagcgcc gcctgaacca cattccgtgt
    14461 gccaagcaca ttgttggtga tggcctcaac agggttggct tccattaatg ggacatgctt
    14521 gtgcgcggcg gcatgaaaca caacctgcgg atgatgctgg gcaaatacag aatcaagccg
    14581 ttgtccattg cggatatcgg caatgacagg gatcagtttc aacgcggcat agtcgttctg
    14641 caattcaagc aaaatttcga aaatgctgtt ttcgccatga ccgagcaaaa tcagttggga
    14701 tgggttgcgg cgggcgatct gacggcacaa ctcacggcca atggagccgc ccgccccggt
    14761 gaccagcacc cgcttccctt caagtgcctg ccctacctgc tcatcgttca cacggacatg
    14821 gtcacggcgg aggaggtcgg taatatccac ttcgcgcaaa cggttgacac tgaccttgcc
    14881 accgagcaat tcataaatcc ctggcatggt gcgcgagggg atgccttttt ttcggcaggc
    14941 atcgttgacg gcgcggatga tcttgccagg cgccgacgga atggcaatga tgacctcgtc
    15001 aatgcgctga gattcaagga catcggcaag ttttccaacc ctgccgatca cagaaacacc
    15061 gtaaatctgg tgattttgtt tggcggggtc gtcatcaagg aaacccactg ggaacagatt
    15121 caactgggat gatttttgca attcgcgcac gaccagagca cccgcatccc ctgcgccaat
    15181 gatgagggca cgtttgccca cccgcagcgc gtcacgcggc gccgacgatt gttcggccag
    15241 aatgcgcaag gcaaaccttg agcctccaat aagcacaagt gaaaaaagcc aatcaatgcc
    15301 aagggcagac cgcggcatgc caggctgaat aaacccgata ctgctgagca ggatcatgac
    15361 gcccgatgtg agcgccgaag cagaggtcac tgcaatggtg atgagccgca attcacttgt
    15421 actggcatac acccataaac ggcggtacag gccaaagtaa aaatacacag gcactttgac
    15481 aagcaaagca accgcacaca tcaccaatgc cgcagggaaa taaaaaggaa gctcgcttac
    15541 atcgagcctt aacgcaaaac tgcccaacac cgaaatcacg atgagggcaa ggtcgccgat
    15601 caagacgaag cgattgcgaa tatgcggacg ggaagacatt ttctaaaggt gaaggaggaa
    15661 gaatgaggga tgaaaagcga tcagttgtca gcgaacatca ctattcacta attactaatt
    15721 actatttctg cccgcgcatt tcttccacag cctcatgaag tccatcagcc agagtcacct
    15781 ggggcttgaa tccgagggtg tccatgatct tctttggagt gccgatggag cggtagatat
    15841 caccagcacg cggctcagcg tggacatgcc tgggcgcgtt cgggaagagt tcgtagagaa
    15901 tatcgagcag gtcaaggagg cgggtttcca ccccggtgca aacgttgaag atctgcccgg
    15961 gcgcagcggg atgctgggaa gccagaaggt tggcctgcac cacatcacgc acgttgacaa
    16021 ggtcacggct ttggccgcca tcaccgaaaa tggtgatggg tttgttatcc aaaatgcggc
    16081 ggataaagat cggaacagcg gcggcgtaca tcgagtctgg gcgttggcgc ggaccgtaga
    16141 cattgaagta gcgcagggcc accacgtcaa aaccgaagaa ctcggtgaaa agctgggcat
    16201 atttttcgtc cacacgtttg gaggtggcgt agggtgaaag ctggcgcagc ggagtatctt
    16261 ccacgagcgg catggcggtg gaatcaccat aaaccgccgc actggatgcg atcacagcac
    16321 gccgaacacc tgccttgcgg gcggcttcaa acaggatgga tgtaccagtc acgttcacat
    16381 cgaaacattc ctgcggcttc tccattgatt cgggcacaga gacaaaagcc gcttcatgga
    16441 aaacaatatc catgccacgt acggcttccg ccacacgcga agcatcacgc agatcgcctt
    16501 cgataacctc aacgtcaagg cccttcaagt tttcacgttt ccctgaagag aaattatcga
    16561 acacgcgcac cttgtcgccc tgtttcaaaa gagcctgcac gatatgagag ccaataaacc
    16621 ctgcaccgcc agtgactaaa taattcattg tttaccttcc tgttcttctt aatacggttc
    16681 cgatggtccg caacacgata ttgaaatcca tgttgagggt acgatgcttg atgtaataca
    16741 ggtcatactc taacttgacc acggtttctt ttacggtagc cacgtagcca taattgatct
    16801 gcgcccaacc cgtcaggcct ggtttcacca gcaggcgcgc acggtagaag ggaatctgtt
    16861 tttggaactg ggctacaagc tcggggcgtt cggcacgcgg ccccacaagg ctcatctccc
    16921 ctgtcagcac gctcaggaat tggggcattt catcaaggcg ggtgcggcgc aagaaatttc
    16981 caacacgggt cacacgcgga tcattgacaa tcgccgggcg ggcttcaccg tcttcttccg
    17041 agttttgcac cattgaacgg aatttgtaga tcatgaacac acggccgccc ttgccgagtc
    17101 gttcctgtga ataaaagatc ggaaatccgc tctccaacac gatggctaat gcagtgaaag
    17161 gaaaaatgac cgcgaagatc agcaaaccca acaagccgcc gacaatatcc atcagccgtt
    17221 tgaacagttc atacaccccg cttacgcgga cctggtccac gaacgagcga atcacccaat
    17281 ctgattcaag gtgttcgacg ggcacacgct gggtcatctc ttcgtacatg atgggcatgc
    17341 gcgtcacttc cacgccttgt tcctgtgcat ccaaaatggt ttgaaaggtc tcccctttga
    17401 tttcgccatt gatggcaacc acaatatccg aaatgcgatg cgtttcgatg atctcaagca
    17461 gtttctggct cgtgcccaac acagcaaaac catgatacga cttggactgc ttgcggaaat
    17521 catcgtcaat gtagccaatg agcaggaagg gcggcgggct gaattttcga tagatatctg
    17581 ccagcgaatg ccccgccttc cctgcgccga cgatcagcac gcggcgcatc aaccctgatg
    17641 aagtgtagag tcgaatatag atggcgcgcc aggccagggt gaggatcgat gcgaaaatca
    17701 aaaacgcacc cacgccgata cgtgggagcg attcagggtc ttgattaaaa gtaaagagca
    17761 gggaatatcc cagcaagccg acaatgggag cgaccgcgat tccacgcagg gtctttctcc
    17821 aactggctga ggtgtgcggc tcgtacgaat ccaccatcag cagcaaccag accagtggaa
    17881 ggatatagaa ccagacggga atcttgagtt caatgatgct ggcggccaaa cgctcggcgc
    17941 gggcctcgga aaatccgcgt tcgaccaaac gcgccacttc ccgcacaagc gagaactgat
    18001 accaggtgaa caaagccgca aacatcgcac cgacagatgc gatcaggtca cccgccaaca
    18061 ggatcgtgcg ctgttcgctg ggtcttaatc gaaggggggg tttgataata tcagccatga
    18121 gtacgtgccg aggcaaagcc cgaaataaaa ctccacagga aaccagcgcc ccatgaaagg
    18181 tgcatggttg caatggcaag cggcagaccg aaaatcagaa aagcatttcg tttttcaacc
    18241 gccagtttca aaccagccag gccaaggaca aaaaaataaa gcagcaactg ggccgccagg
    18301 gcatattttg caagcccgaa aaacaaggat aacacaataa aaaaaatcag ggatgacaca
    18361 aatacgggcg gcaaagcctg ccgccagcgc aatgtgtgtg gatagcgctt aagcattttc
    18421 agcttccaaa acccgtaccg ccagtattgc gccgacaggc tgcccagtgt gcttctcgaa
    18481 aaatacaccg aacgaatcga ggggtcaagc cagaccgtcc cgcctgattc gcgcacacgg
    18541 gtgttgaatt cgtaatcttc atttgccagc aaagtctcat cgaacaggcc gatcttttcg
    18601 atgagtgcac gtcggaacga accgaacggc accgtatcca ccgctcccgc actggcattg
    18661 agccgataca tggcatcgcc cacgcccagc ggatgcgccg ccgcaaacga gatcgctttg
    18721 gcaatggccg tatcagcacc tggccgaatc tcccacaccc ctccgatatt ttcgcccttt
    18781 cccgcttcat gtgccgccac acaacgctcc acatattcag ggatgggcat cgagtgagca
    18841 tccaggcgga caaaaacatc gcctcgcccc tcacgaatgg cctgattcag tgctgaagga
    18901 atggcccttg ccgtgttatt gaccacccga atctcaagat ctgcgtgttc cttctgaaac
    18961 gcctccacca cctcccgcgt ccgatcggtg gacatgccgt ctgaaatgat caattccatc
    19021 tgtgcgcgcg ggtacgtctg cgcaaaaacg gcattcaaca aatgacgaat ggtggcttct
    19081 tcgttataac aagggacaat gatcgagaca aagggcggca ttttaaagga aaatttcccg
    19141 aaattggtta ttttctgacc agcggcagga acacctcaaa ggtggcaccc tccccctgcc
    19201 tactgcggac gcttattcta ccaccatgcg cttgcacaat ctcactggca atggccagcc
    19261 ccagaccagc accatgcttt gcaccaccct tgcgggcggg gtcggcctga taaaaacggt
    19321 caaagatatg cttctgcgct tcggcaggaa tgccagcccc cgtatcagta accgcaacac
    19381 gcatttcgcc atcaagcatg gaagcctgca acaaaatgga acctcccgac ggcgtaaatt
    19441 tgagggcatt atccaccagg ttggtaaaaa cctgcgccaa cctgtcaccg tctgcagtga
    19501 tggatggcag gtctgcggcg gcttccactt gaatacgcac ccccgcacgc gtggattgcg
    19561 gcgcgaattt ctcagccacc gcattcaaca aagcagacat cttcacagga gacatcgtaa
    19621 tatctgccgt gcctgaatcg agccgtgcca gatccaagag atccagcacc atgcggtgca
    19681 tccgccctga ttcgtcgtag attacttgag cagccttctg acgcgcctct tccgaatctg
    19741 ctgtgccgtc aagcattgcc tgcgcaaatc cctgaatgga agtaagcggc gtcttcaatt
    19801 catgagagac attggcaaca aaatctcgct gtgaccgttg acttgcctga gtccgcgcga
    19861 ccatggaatt aaaagcacgc gtcaattcct ggacctcgtg cgggccgccg atttcaacag
    19921 gctttatctc cgctgacggc atggcacgcg cggccgccac cactttttgc agcgggtcgg
    19981 cgatccaacg cgcgaagaaa aaggcgacca ccagagaaag cagtaaggca agcaatccgc
    20041 tcctgaaaat gagcggcaga aaatcatctg tgaaaacatt cgaaatggag agtttcggtc
    20101 gcggggcagc caccaacaag tagcttttat ctggcaagat ctcaaccgaa tacaaccatg
    20161 ccttcccatt ctcatcacgg accaatggga cattccgcgc aaagactcgt ttttcaggga
    20221 acggcaaaac aggctctttg cttgagtagg tatctgatag cacttgcttt tcccgtgagt
    20281 actgaacaat ccgtacatcg aacgtcagag aagccttctg cgctgcaatg gagatcggct
    20341 gggcgttggg cagcctggaa ttgtccagta ccacaccttg cacagcccgc agtctttcaa
    20401 gggtttgacg atacaacaag gggttggtga gcaggtaggt aaacagaatt atcgccacga
    20461 cacccaatgc cgtgacgata agaagtgcat aactcaacca aaggcgggaa cgcagggagg
    20521 aaaacattag ggttgaaggt tacaggttga aggttataca acaagtttgt aaccgacacc
    20581 tgtgacggtt tcgattttta tgtttgagcc ttcaagcttt ttgcgcaaat gggcaatgtg
    20641 tacatccact gtgcgggtct ggccgtagaa atcaaatccc caggccagtt gcaataattg
    20701 ctcacgggtg aagacacgcc caggctgttc tgccaacgta aacagcaggt caaactcctg
    20761 cgtgcgcaga tcgaggattt gcgaggcgat ggaaacttca cgagaaacag gatcaatggt
    20821 caggtcgccg cgatgaagcg gttttccatc cgcttgtttt ttgccgtcac tcctgcgtaa
    20881 aatcgccttg acacgcgcga tcaattcacg cggattgaaa ggcttggtca ggtaatcatc
    20941 tgcgccgagc tcaaggccga ggatcttatc aatgtcttca tcgcgggcgg tgagcatgat
    21001 gatgggcgtc tggtctcctt tggcgcgcag ggtacggcag acctcaaagc catccaattt
    21061 tgggagcatc acatccaaca cgatcaaagc agggtgatgt tttgtaacgg cttcgagtgc
    21121 ggcttcgcca tctccaacct cctgaatacg aaatccatcg cgctcgaaat acatgcgcga
    21181 aagctggatg atggaaggtt cgtcgtcaac aaggaggatg agttcggaag ccataatgag
    21241 cctgatgatt aattagacag ccagcacaac cgcttcaact tcaaccaggc cgcccttggg
    21301 cagggccgcc acagcgatgg tgctgcgggc aggcggattc tcgccgaaga attcggcata
    21361 aattgcgttc atctttggaa aatcggccat gtcttttaga aaaacggtgg tcttgactac
    21421 gttgtccatg ttggagccag cagcctcgat cacgtttcgt aaattactca acgcctggcg
    21481 tgtctgttct tccactgcac ctgagatcaa ctcacccgtt gtcgggtcaa tgccgatctg
    21541 ccctgcggtg aagatgaagg catctgtgcg gatggcttgc gaatatggac caatcgcctt
    21601 gggggctttt tcagtttgaa ttactttctt tttcataagt acctctttga gaaaattttg
    21661 caaataataa cgagtattcg cacgcgttga ttttaataca gatttggctt tgcaggccct
    21721 ttaatgaaaa tgacatgaaa accaaacaca aatgagcgaa tccatctgct gcaaaatatt
    21781 ttcaaagaag acaaaaacag gaccagcgcg atcttttgcg ttggtcctgt caacatcaca
    21841 ctacgtaccg cgttcagaag ttccctcagc gggctggttg gcttcacgga aagccttgat
    21901 cgcatcacgc agggccttga acgtgccatt catggaggtt tttacctcct gcattttggt
    21961 acgcatcgct tgcacagtgg ctttggcctt ttcggcatcg gtcacttttc cgtttgcatc
    22021 aaaaccctga tgcgcactga caatgccctg aatgctttca taagcagggc gggcattctt
    22081 caccgcggct tcgtaggcat ctaacgcggc ctgcaaagcg gtgacatcct tgccgttggc
    22141 ggcggcttta tcaatacgct cctgcacttt ggcagtgaac tcatcggtct tttcgaagcc
    22201 tttgccgacc cgctcatacg cctgcagttg acgtgcccac atctgctcca gcttttcgtt
    22261 ggtcacctcg ccttcagcac tgccattcat tgcaaatgcg ctggaaaatg gaagcgcagc
    22321 caaaacgagg gcggcggcaa ttgccaccaa tattgttttc ttgaacatgg ttttcatcgt
    22381 aaacctcctt tcaggttacg atgaaatttt agatgcggca ggtaaggtgg atatggctgg
    22441 attgtaaaaa gattgtaaat tatggctttg caacgaaaaa gattcaggga aaacttctgc
    22501 aaagtcagta atccgccata ttagccgcca gaccaagctc taatcgtcat ttataacaaa
    22561 tgcctgttga gcagcgggtg ttgaaaccag caagaggata atcatcaaca cagcagagga
    22621 gatacaccac atgcaagtag agccaataac gaaaggctcc aaaaacgtca agtaaatctc
    22681 aaacaacaca ccaaatatac acatcgccca aacagccaat gccgccattc tttttattgc
    22741 atcaggaccg aagtgtaaaa caagccagcc tgcaagaatt ccgacatacc cagccaggcc
    22801 taaaacacca actggcaaaa ccccaaaaag aacagcatac ttactgtttt gaacagcagc
    22861 acacccatca gatgggccac acacaactgg cgcgtttgtt gcttcaacaa atgtcaaata
    22921 gccagccact gccagcccac cgacggttga aattaagact gaccacttgt accaatctgc
    22981 gaccagaaag tgcgaagatt ttccataaat ctttagaatt gggacaacaa tgttaagaag
    23041 taaaaccgta aagacgatcg ccgcaaacac aaaggtaact ccttttgttt gcagcaaatc
    23101 cagcacggct ggtatgccaa gaatactgaa tacagggagc agattggcat accagccttt
    23161 ttccgtttca acaggaacat ctcgcatact taatacgatt gacgtaaccc aggtaattat
    23221 caaggctaac agcacaataa ctgccaaagt atttggcaat ggtgatttca tgaaaatgtt
    23281 gatcataaac ttccattcgc ttaaatatgc caggtcccat tgccatggca ccaacaacca
    23341 gattatggct tgacatacac cgtatattcg aggatatatt ctccagcttc gaaattggcg
    23401 ctgccatcgt taatggcggc ggtgaatgtg gcggttgtgg agaggtgatg ctcgcccgaa
    23461 gtccactcac tgagggaggt gtagatgagg cggcagactt tcccgcctgt ttccagttcg
    23521 agggttccga atgaatcagt aggcacttcc tttccatcaa gcatgaactg catatcgatc
    23581 ttctcgaaat tggatgcgac tgtggcggca tcaacggcgc accaggaata gccccagatc
    23641 acaggttcgg gatcggtcat ggaaatggaa taggttgcag ttccaggctg ggcaaaggtg
    23701 atggcgggat catagctttc gcgggcaagg tcttccaaaa atggagcacc cgaagaaagt
    23761 gcagactccg cttcggtttg cgaggcaacc tttggggccg cactgggggt tacgccagcg
    23821 ccaaacggct ggagcactgc atcaatgcct gcttgtagaa caatgtcttc gatagccgta
    23881 gcagtttcct cgttgatggg gattcgcagc gtgggcggca ctccctgccc ctcgaggaag
    23941 atgctgccat cgggcagggt aaagcgtcct gtgggaaccg tcacgctgaa tccttctggt
    24001 agattgaacg tgccgcgtgc ggtctctgct tcaacgccgc tggttggcgt gaggccgaca
    24061 acaaccatcc ctggtacctg gctgaacccg taggcttcga tctcgcaggc actaaaacat
    24121 gcagggccaa ccaacaggac ggtcttttcg aagcgatatt ggttttgatt gggcaggaat
    24181 ttctggcgca ggccttcggg ttcaaacttg cccgttttat cactgtagta ttcgagttgt
    24241 ccgagtggaa tttcctgctc ggtgaagaaa ccagccagcc cgagcggcgc accgccattg
    24301 ttgtaacgca tatcaatgat gatgcccgcc acttcattcg cttcgaattg tttgagcgca
    24361 cgttcaaaaa ggcggatgac gagattgagg tcatcgtaat tgctgttgat gcgaatgtag
    24421 ccaacattat cgttgttgac aggaatgatc ttcaaatcca ctggcaggag tgaactgtta
    24481 tccacaccga agtacagcga agtgcggctg aaactctggc gttcctcaat ggcagtcagg
    24541 gtaaccacct gcggaatgcc atctgcattg gcaaatttca ccgtggcttc tgtgccaggc
    24601 aaggcacgca acagataacg agatttctgg taacggatcg caaactctga ggattgattg
    24661 gaatacgatt gcgccccgga gatggcatcg gaaatgggca ggccattgaa ttcggtcact
    24721 tctgcaccga cctgaatacc agccgcctgc gcggggccgc cgtccattac gaagatcacg
    24781 atcacacggc catcatccaa ttcacgaatc gcaaaaccgt atccgcctga aacggctgtg
    24841 gcgaaatcct gctggaaata ctgcccgccg ttgaggctga catggccgtc atcgaaagcc
    24901 caggtcagat cgcgcagggc aaggtaaaaa gcattcgggt cgctggtctt ttcggcctgt
    24961 tccacgcgcg gtttcaattc agcgtacaag gcatcccaat cgggggcttc gccgggaata
    25021 tcagcaaagg catattcatt gcgaatcact tcgaacattt tgtcaaaggc ttcggtgtaa
    25081 gacagggtag agtaatcctt tgtggcgatg tcttcaggtt cgtacaaagg cagatctgag
    25141 atggcatctt gagtaaccgc aaaaggaact tgatccaggt caacgattga atatccctgc
    25201 gaaatgggag cgacgggatc atcagcagtg aagagcaagc cgtcatctcc aaaacttgtg
    25261 gggaattgct ggttggcatc ttcggcccac accaccagtt tcccgccaat gacttcatcc
    25321 ttgttttccg agtcggtctt taccgaagcc aggtaggaag gccagccgcg cgagcggtca
    25381 tcaccttcag cgaaagggcc gccgtacaca ttgggtgaat atgccactgc aaagatctgt
    25441 acacccgcat cagcggcgcc gttgttatcc acatcgttga attcgccttg cggtatggca
    25501 ggcaggctca gttcatacgt gccgccgagg gcatctgcat caaaggtcat aaacccaagg
    25561 acctgcgaat caacgggaat ctcccattcg aggtcacgta atacaaatcc gtgcatatcg
    25621 gtcaaagcaa cagcattttc cacataatag gttgcaagca caaaatcatt ggtaacggta
    25681 taagaacctg tcagttcgta gggttgagac attgccccca gaaaatcccc ttcctccacc
    25741 gcttcagttg gcaccgcagt gggtgtacat gctgcaagcg cgaggctcac tataattaaa
    25801 tatccaaaaa ttcgaaacaa cgtgggcttc atgttttctc ctttgagttc ggttcccata
    25861 attatagccc catggttaag cggtagatta taattttcgg catggataat gtaaccacac
    25921 aattacaaaa agaatttgaa aaagcccaac aagcccgcat cgccaaaaac gaagggcagg
    25981 cccgcgtttg tgcccgccgt gccgccgggg tggccatacg ggattacctc acccgcaaag
    26041 gggacaaaat cccaagcatg agttcgtacg atctgctgaa tcttcttaaa gacaacgcgg
    26101 ctcttcctcc tgacctgaaa ctggccgttg acaacctgac agtgcgcgtc actgaagagt
    26161 ttaccctccc tgttgatgcc gacctcattg cagaagccag aaaactgtgc aattggttgt
    26221 tgagcaatta atcgccatct cccaatcttt ttacgaaaac aaaatcaccc acaaaaggca
    26281 gagccttggt gggtgatttt ttcatattct ctattttttc aggcaaagcg gtcggtctct
    26341 ttcagatgac cagcttacat cctgcgccca gccaggtaat acaaggcgcg tgcgacagtg
    26401 atgagcagaa gcatccccga aaccacaccg ccaactacaa agaacatccg ccgccaatca
    26461 gtgaagcgca aagtgatctc actgcggctg ggcacacgga tccgtgctcc aaggcgctgg
    26521 acaagttcct tcggaggcga gatcggcttc aaggtcccag ccagatgcgc ttcaagagta
    26581 tccaaatctt ccagtgtggt ggatgtgttt cttctagtca tcattcgtaa atcacctgtt
    26641 tattctgcgg ggtaaaggat caaccccacc gtgccaggcc caagatttgc agcaatggag
    26701 atggataaat cgctgagcat ggtttcttta tggttgaaat gagatttcgc gtcatcgagc
    26761 aaaccctgac ctgatttggc gtcacgagca tgcaccactg ccagatagac aggtttcttg
    26821 ccgaactctt cttcagcaat ttcaatcaca cgatcgagag atgccttgcg ggtacggaca
    26881 cgttccgcca tattcaaatc gccattcctg agaattgcga ttggcttcac gttaagcaaa
    26941 gaggccaatg ccgcctgcaa agttttcaca cgacccgaca tcttggcata ttccaaagta
    27001 tcaagggtca gaatgacacg gatcttatat ttgatgtctt caaggtaact tacgatctca
    27061 tccacggttt ttcctgcgcg ctcaagcctg cgggcttcac ggcacatcat accgatgccc
    27121 aatgaaccaa ccgctgaatc gatggggata accttgaatt catccttcaa ttcttccgcc
    27181 gcggcgatcg ccgatgcata agtacccgac aattttgcgg tgatatgaat tgaaagaatc
    27241 gtatcgcctt tctgggcgat cttgcggtaa aactcaacaa actggtgcgg ggagggttgt
    27301 gaggtcttcg gaatgcgttt ggtgtcatcc accagtttat aaaaaccctc attgtccaat
    27361 tcgacaccct ggaggtacga tttttcccca aacaggatgt ttactgggat cacttcaatc
    27421 ccatactcct gcccccatgc agggaggata tctgctgcgc cgtcggtcac aattctcaac
    27481 atggtctact ctccttcgaa attaaggttt tggtcttcca gttggtcacg caaagccaga
    27541 agtgtacggt gatacaaact cttcacagct ccctcgcttc tacccatgat tgcgccgatc
    27601 tcggcattgg acatattctc gacaaacttc aagatcagta aattttgtcg ttctgcaggc
    27661 agggttcgaa tcattttcaa caacacatcc tgctcttgtg aacgaacgag gtttctttct
    27721 ggatgatcgc ccttggttgg caaaatgggc aggtcatcaa gcggaatctc ctgcttgcgg
    27781 ctgcgatcac gatgccagtt tgccaccagg ttgtgagcga tccgatacaa ccaggctgag
    27841 aatggcacgc cccggtccgt gtaattcacg atatgcttca tcgcccgttg aaagacgcgg
    27901 gcggtcaaat cttcggcatc gtgagtattc ccggtccgat aatagacata attgaagatg
    27961 cgctctacat aatgggcata cagttgacca aaagaatcac ggtcaccctg ggaggcccgc
    28021 aagagaatct cttcttcgtt gtattccata tccagcaaga ttaaaacctt accggcgggt
    28081 ttactaaaat aacaccgccg gatttgagaa gttgcactga gtataacgcg aaatacaagg
    28141 ccgaacggca agggatgggc aatgaaaaaa acatgcaaag aataaaacat ttgtgctaca
    28201 attgctcccc atccaaggag aaccatgtca accaatgtca ttcgcgtcgt gggcgcacga
    28261 gtccataacc tgaaaaatat caccgtggaa attccacgtg acaagttcgt cgtcattaca
    28321 ggcttgtcgg ggtcgggcaa gtcatcactg gcctttgaca ccatctttgc agaggggcag
    28381 cgccgctatg ttgagtcgct ctctgcgtat gcgcgccagt tcatcgggca gatggataaa
    28441 cccgatgttg attacattga tgggctttcc cctgccgtat ccattgacca gaagtccaca
    28501 tcccacaatc cccgttccac ggtcggcacc gtgaccgaga tctacgatta catgcgtttg
    28561 ttgtttgcgc gtgccggcat cccacactgc cccatctgcg gacgagaggt cgtcaaacaa
    28621 tcggcacaag agatcgtgga caagatcgaa aaactgcctg aaggttcacg cgttttgatc
    28681 ctcgctccac ttgtccgcgc ccgcaaaggg acctatcaag ccgtcttcga agagattcgc
    28741 aaagcaggtt ttacgcgtgc ccgtgtggat ggcaacaccc attccctcga tgaagagatc
    28801 gaacttgacc gttacaaaca gcacacgatc gaagccgttg tcgatcgcct cgtcatttca
    28861 caggaaggcg ataaagagga aaaaaaatcc gctcgcaccc gcctgaccga ctcagtcgaa
    28921 actgctttga agtttggtga aggctacatt accgtcaacc tggtggataa aaaagaagac
    28981 atccaattct ccgaacacct cgcctgcccc gaacacggtg tcagtatttc agagatcgag
    29041 ccgcggacgt tctcctttaa cacgccgcag ggtgcctgcc cggactgtca ggggcttggc
    29101 tcaaaactgg aaatagatcc cgaacgcatc ataccggacc ctgaccggtc tctgaacgag
    29161 ggagcgatct ccagcatgga gtggagtgga ccgaaagaag aaggcggcta ctattggcag
    29221 ggccttgaag ccgccgctca cgcctacaag atcgacctca acaaacccgt acgcgaactc
    29281 accaaggatc agatgaaaat catcctgtac ggcacgggcg aaaaacagat ccaaatgaac
    29341 tacaaaaact caaatggaaa tgaattcaaa ttcacccgcg ctttcgaagg tgtgattacc
    29401 aacctcgaac gccgctaccg cgaaacgaat tccgagtgga tacgcgaaaa aatcagcgaa
    29461 ttcatgtcag accgtccctg tcccacctgc aacggcaaaa ggctaaaccc cgccgcgctg
    29521 gccgttacag tggacggtgt gaatattgta gaagcaaact cctggcctgt tttgaagacc
    29581 cttgattggg tcaaaaagat ctccggcaaa ggttcgccgt tgacctcaaa gcaaaagaca
    29641 attgccgaga gggttatcaa ggaaatacac gaacgcctta acttcctcgt caacgttggg
    29701 ttggattatt tgaccctgaa tcgctccgct gccacgcttt caggcggcga agcacaacgc
    29761 atccgtctgg caacacaggt ggggtcaagg ttggtcggtg ttttgtacgt tttggatgag
    29821 ccttccattg gcctgcaccc tcgcgacaat tcacgattgc ttgaaacgct gaaaggtctg
    29881 cgcgatctgg gcaataccgt tttggtagtt gaacacgatg atgagaccat ccgcgaatcc
    29941 gattggatca ttgacctcgg ccctgccgca ggtgagcatg gcggtgaagt catcgctgaa
    30001 ggtacaccga aacagatttt ggcgcaccca aagtcgttga cgggagccta cctctcgggg
    30061 cgaaaacaga tcgaagtccc gaaaaagagg cgcgaaggaa atggcaaggc gttgaaaatt
    30121 gtcaatgcaa cggcaaacaa tttgaagggc atcaatgtct ccattcctct tggaaagatg
    30181 gtttgcatca ccggagtttc agggtcgggc aaatccactt tgatgagcga cattctgtat
    30241 aactcgctgg ccgcaaaatt gatgtcggca cggacgagcg caggagagca cgaacggatc
    30301 gaagggatcg aacacctcga caaaatcatc aatatcgacc agtcaccgat cggacgtaca
    30361 ccgcgttcca acccaggtac ctacaccggt ctttttgatg agatccgcaa gttgtttgca
    30421 gaattacccg aaagcaaggt acgtggatac aagcccggac gcttctcctt taatgtccat
    30481 ggcgggcgtt gcgaagcctg tcaggggcag ggtgaattgc gcatcgaaat gcagtttcta
    30541 cccgatgtgt acgtgccatg cgacgtttgc cacggcaagc ggttcaaccg cgaaaccttg
    30601 caggtgctct tccacgataa atacagcatt tccgacattc tggatatgac cgttgaccac
    30661 gcgcttgatg agttcaaaaa ttatccaccc atgcagagca agttgaaact gctgcaagaa
    30721 gtggggctgg gatatgtgcg ggtcgggcag cccgccacca cgctttcagg tggtgaagcc
    30781 cagcgtgtga aactctcaaa agaactctca cgccgagcca ccgggcgaac cttgtatgtg
    30841 ttggatgagc cctcggtagg cttgcacact gccgacgtcc acaaattgat cgaagtgttg
    30901 caacggttgg tggattctgg caattcggtc gtcatcatcg agcacaacct cgacattatc
    30961 aagatcgctg actggctgat agacctcggc ccggagggcg gtgaccgtgg cggccagttg
    31021 ttagccgaag gcacccctga acaggtgatg aaggtaaaag aatcctacac gggaaaatat
    31081 ttgaaagccc atgtaaagta ggcagagaaa cccacagaac aggtaaacaa attgtttacc
    31141 tgttctgttt tttatcggtt cgtccgctat ccttgtttga gcctccttac gcgattctcg
    31201 ttttcaggta gaattatttt atatcgtgcc gctgggaccg accaacaaga aggcacttta
    31261 tcaaaaggaa caattcatga ataacagacc tccgatttct tcttcaccca acccgggcac
    31321 catcagcctg ctgcgcaagc gcaagcgcaa ccctaacatt gtcctaatcg ccgcaggcgt
    31381 actggtgtta ggcggcatca tcatgctggt tgtctggctg accggtgaca gcagtccact
    31441 taacgcgctt ttggctactg aaacccccac cccaaccatg acgttcacgc caaccagcac
    31501 atccaccccg acatcgacgg cgaccgcaac agaaacaccc accatcaccc ccacaccgac
    31561 gttctccacg atcttcaact acactgtgga agcgggcgac tcgcttttct caattgttga
    31621 aaaattcaac cttggtgaaa atggcgttgc cttgatcttg ctcctcaacc cctatggcgg
    31681 catcgccgac tcaggcttcc ccatcggcgt ggaccctgca acgctgaaca ttctgccggg
    31741 acaatccatt gtgttgccct atccgggcat gccattgccc accgccaccc cgctcccagc
    31801 caacctggcc ccggggacca agatcgaata catcatccaa agcggtgact cactgggcag
    31861 tattgccgcc gcctttaaca gcaccattga tgagatcatc gcggccaatg gcatcacaga
    31921 cccgaatgcg atcaaagtcg gtgatacgct gcaaattcct gtgaatgtcg tcacagctac
    31981 ggccacacgc ccgcccacca gcacccccat tacaccgggc ccaggcacat tgttacccac
    32041 ggtcacattc acaccgatca acgccctgcc tgccacggcc acagcgactc ccaccccgta
    32101 atttgcccgc aaaattactg gacaaatgta tttccctcat gtaaaatacg gcgctgtcat
    32161 aaaatgaaag ttgtaacatc tggagaataa ccttggctaa cattaattca caaatcaaac
    32221 gcaatcgtca aaatgaaaag cgtcgcatcc acaaccgcaa cttccgcggt gcggcccgta
    32281 ctgccatcac agccgcccgc agctcgctcg caaagagcgc tcccgaaaca caggaagata
    32341 tcatgctggc ggtcagcgcg ctggacaaag cagcccagaa aggcgttatt cacccgaaca
    32401 atgcctcccg ccgcaaaggc cgcttgatga agaaattggc cgctttcctt tctgcgaagt
    32461 ccgaaaagaa agcctagtct ttccttcaac accacaaaac gggaacgccc tcaaaacggc
    32521 gctcccgttt ttgttatctc aatctctccc ctaaatttgg ggatggcgtc tataatcaaa
    32581 ccaatgttta caaaagaaca acaactcatc gaagaaaaga tcaaagccta ctgccaggcc
    32641 aacgacattg cactggcaga actcaaatgg cagcccatcc ccttttcggg cgaatggggt
    32701 ttcgccacgt cgttttttca gaccgccgcc aatgaagtga aactcggcaa gggcaacaaa
    32761 cttccggtgc cgcaaaaggc ccaggaaatc gccgagcagg ttcgaggtca gatcggaagc
    32821 gtggatggga tcagtcacat cgaagcggtg aagggatatc tcaacgtcta tttcaagaca
    32881 tccgattacg cccaacgcgt tgtggatgaa gtgctcgcct ctggcctgaa ctttgggcgg
    32941 ggtgcggcga aaaatgaaag agtcatggtt gaatatgccc agccgaacac acaccactcc
    33001 tttcatattg ggcatgcgcg taatacgatc ctgggtgaag tgctggcaag gctggtggaa
    33061 ttcgcaggct tcgatacgat ccgcgcttca tatcccggtg accttggact cggtgtgatc
    33121 acagtgatgt ggatctacga aaagttttac aagggacagg aacccacagg cattcacgag
    33181 cgcggacaat ggttggcgaa gttgtacgtg gaagccacgg ccttgcttga aaagaaggaa
    33241 aacgaaacgc cagagcaaac tgcccagcgc gaagcctatg aagctgaacg ccgcgagatg
    33301 tatcgcaaat gggatgcagg cgatgaagcc gtacgaaagt tgtggcacga gacccgcgaa
    33361 tggagcctcg aagaattgcg cgatgtactg cggatgctcg atgtaaaaat ggatgtgtgg
    33421 ttctacgaat cagaagtgga cgaaccctcc aaagccatcg ttgaagaatt gatcgcaaag
    33481 aacatcgccg atgacgaacg cccacagggc ggcgcggtga tcgtaaagat cgacgagaaa
    33541 ctgggcctga ccaaggaaaa gtatcgcacc aatgtcattc tgcggcgcga tggcaccacc
    33601 ttgtatctca ccaaagacct tgcgcttgcc aaagtgaagt ttgaacaata tcacgttgac
    33661 cgctcgatct acgtggtgga tgtgcgtcag tcactgcatt tacaacaggc ttttgccatt
    33721 ctcaagttgt ggggcttccc gcaatcagaa aagtgctacc acctcggcta cggatttgtg
    33781 agcctgcccg aaggcgccat gtcggcacgg cgtggacgtg tggtgctctt caaagatgtg
    33841 gcggatgaag cagtgaagcg tgtcctttcc gtggaaaccg aaaaaagcgc cgatatcccc
    33901 gccgaagaac gtgaaaaggt cgcctaccag atcggcatgg gcgcgctggt ttattccatg
    33961 ctttctgtgg acaacaacaa agacatcgtt tttgacatca acgaagctct atccttcgat
    34021 ggccgcacag gtccgtatat tcaaaatgca catgtgcggg cgaattcaat tcttaagaag
    34081 gcaggcgttt caacgtttga acgttcgaac gttcaaacgt tcaactacga ctttactaaa
    34141 catgagatcg aattgatcga acagatttcc cgcttcccaa aggctgtgca acaggccgcg
    34201 gaagaatatc gtccgctggt gatggcgtcg tacgcttatg aactggcgaa caccttccac
    34261 tcgttctatc atgcggtcaa tgtcatgcag accgaggacg aaaacaccaa aaatgcccgc
    34321 ctgcgactgg tcgctgcggc caagcaaacc attgccaatg cgttacgcct gcttgatatt
    34381 caagcgcccg atgtgatgtg atttttgtat tgaagaggct gggtggttga aactacccag
    34441 cctctttttt atttcactgt gatcgttgaa ggtatatcgg cgttttcagt acagtagaca
    34501 taatagaccc cagcctcagg gaagcttgtt gagaaaacag tctcggggtc aatcacagat
    34561 agtcccgtat cagtgacctt gccattttca tccaggaatt cgatctttat cggcagagat
    34621 accgtatcga cattttccca ggctatggtc atcccaacct cagctactat ggaaggaca
//
LOCUS       DAOQLC010000033        34359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_388_length_34359_cov_25.348006, whole genome shotgun sequence.
ACCESSION   DAOQLC010000033 DAOQLC010000000
VERSION     DAOQLC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_388_length_34359_cov_25.348006"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PL000_07890"
     CDS             <1..1044
                     /locus_tag="PL000_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC88858.1"
                     /translation="MSKICIVPKVDGIGGMASFRLKFEQGLRERGVDVTHDLSEDSDA
                     ALVIAGTRNLIPLWRARQRGRRVVQRLDGINWVHRKRNTGLKHYLRAEYGNLVLSFIR
                     RFIATDIIYQSEFSRDWWERKYGKTRVPFAVIHNGIDLNAYSVKRDNIPLYPPFRLLL
                     VEGNLGGGYDMGLDNAIALTETLIEKHNLPVELMAVGKISDDHRQRVEAKTRAPIQWM
                     GSVPRERIPEIMRSAHLFFSADLHPACPNSVIEALACGLPVVGFDTGAVKELVIGDAG
                     RVVPYGSNSWNIEPPDISALAVVAKEILQNQPHFSQAARTHAESALGLDKMVDAYLKV
                     LLTTKGHEGTQRF"
     gene            1108..>1529
                     /locus_tag="PL000_07895"
     CDS             1108..>1529
                     /locus_tag="PL000_07895"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC88859.1"
                     /translation="MDNEIHEFLLKHLHGIMNNDIASYHETTAEDLTLYEWWVTPHRL
                     DGLPFHEFMMNSNASRGSVFGAEDQGQKAQSRFDLANLHIQRYGDTAIASYTLLISTA
                     LPEGVKVAAHNESRVMVKMDGKWKVVHVHKSPAYSAPHV"
     assembly_gap    1530..1629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1630..3051
                     /locus_tag="PL000_07900"
     CDS             <1630..3051
                     /locus_tag="PL000_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88860.1"
                     /translation="YDFLWLGIALFPLLIIAVLLPIQPHDYWWYLRLGKDILQTGSVP
                     VVDTYSSIQAGQPIVYQSWLSAVVLWWVFKAGGVPFTIFLVAVLIGLTYALLWLLMRT
                     SGIGPRMATLLTIIAGLAGSNNWGVRPQLFAYPIFLAVYWLLLKWHHREEKSLWLLIP
                     LSLAWTNLHGSFILFFVLVGIAFLFGAGERKKLLWVALASFAISFLNPRGYVLWGSVI
                     GTFTAPGIRDLSPEWLPPLNQGWQMNIFFAWLILLIPLAAASGTRKRLTAFEWILFLT
                     FSWMALTGIRYVIWDLFLIAVLTAFLMPETLIQTFDQPQEVKIPAMNFALGAIFILLP
                     LMLLPGLRDAWWQESPPALDPVTTPVDAAKWLEEHDQFPGPMWNDVVFGSYLIHALPS
                     RPVWIDTRIQVIFTAEQAHQYLFVQSAQPGWGSLLKEKKVNLLFLAKTQPAIVDAVQN
                     SSEWCEQYHDDVAFIFTRCEPLP"
     gene            3048..4130
                     /locus_tag="PL000_07905"
     CDS             3048..4130
                     /locus_tag="PL000_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC88861.1"
                     /translation="MKNFPHRLGLQQRVLPSYRVPFFDLLAQSCESMSLFAGQARPVE
                     MIAGGRPQVAAYHEAKNIHLFSGAFYLCHQQNFISWLEEWHPHTLIVEANPRYLATSA
                     AVNWMHARGRKVIGWGLGSPPLSGPLAGFRQKSRISFLSKFDALLAYSQRGADEYAAL
                     GFPREKIFVAHNSVSPKPTFNLQPATFKPTPSILFVGRLQARKRVDHLLRACAEMDSK
                     PRLMIVGDGPERAALESLAKDVYPSAEFIGAKHGADLKPYFEQADLFVLPGTGGLAVQ
                     EAMSYGLPVIVAKGDGTQDDLVRDGNGWQIQPEDYGALVSTMKNALSDTARLRKMGTE
                     SFRIVSEEINIQKMVEVFVDALNSVA"
     gene            complement(4163..>5758)
                     /locus_tag="PL000_07910"
     CDS             complement(4163..>5758)
                     /locus_tag="PL000_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88862.1"
                     /translation="AFRFDQEVILKTETERLNILILVFGLLLGAYARFLPASMVGFPV
                     TDGGLFYEMVKAIQAGGYNLPSFVEYNGISIPFAYPPLGFYTAALISDIFNFPLLEVF
                     RWMPAVGSVLFSIAFYPMAAAILKSPLKGSLATVFFALLPRSISWYIMGGGITRVLGH
                     IFLMLILFSAYKLFTAPSRKYLVLTILFGSGVILSHPEASLHTVFLCAVLWLFYGRSK
                     EGIKNASLVALGVIALTAPFFLTVISRHGLQPYLSAAQTGFQNPLAWVAILTGSFADE
                     KFVTVLSALALLGFVVVIARREFLLVVWLFLPAVVDPRSAASISIIPWAMLAAIGFVD
                     IVIPGLYALKHNLQEAGEIESNWSTYFIKSSAIKFALTALIFYAFFGTVLSDQVYPKV
                     SLTSSDRAAMEWVAGNIPPQSRFLILTGETEAFADAVAEWFPVLSGSYGIATIQGYEW
                     MPDKIFLTKLDEYYALQACMDKTSTCVDEWALKEDHPFEYVLISRDGLSGNKNSLLIS
                     LEQSGGYQLIHEETDVYIFEKNP"
     assembly_gap    5759..5858
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5932..6633)
                     /locus_tag="PL000_07915"
     CDS             complement(5932..6633)
                     /locus_tag="PL000_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88863.1"
                     /translation="MKAFHSFVLSIPYLFLKKIPYAWVFVIALWAWPPVVSGIFVGII
                     LLGLWFMVMQQKAWEAEIAREYRVLYRDQPHAPVMYQVRNFVLVCAAGALIGWVADGK
                     LGLSSLQWTLMLIGVMLLYKDAMLFGAGTVYLVTNKGIAVRYIPGHVDYRLFFGYDEI
                     RNVERIGPVKSLPMAWSVLTPQRKILENGFLLKPKKLNGFTKTIEEVVLCPADPEGFL
                     KHMPPSISVKEAVTR"
     gene            complement(6642..7802)
                     /locus_tag="PL000_07920"
     CDS             complement(6642..7802)
                     /locus_tag="PL000_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC88864.1"
                     /translation="MTFQLVYYSQQDPKWKEDILGFGDSRDTIGYVGCALTSVAMLIS
                     GHGFIETPKTLNQKLKNVNGFVSAGIRWDTVNQIYPQINVKSNISCGNTDAPLGLIDA
                     SIAAGQPVIVMVDSTPAPGLLTHWVVLYGREGNDYLMLDPWPYQTDVTKKTYLMPRYS
                     QGNSLERAIMHVIAYECFTASGGITQPIETDQTTPTTGTTTTSTTLARVKADLTWGLN
                     IRSSIDTSSSSNIVAVVSAGTLLTLLGADDISKIGAVNQWVRVNDGKGHEGYCAAWYL
                     EKVQVAAPVVETESGSTSTEEAGGSTSTPPKPAPMIVTVKARVGRLGAKVYKTASAKS
                     EIVSTEKMRAKLTVVEDAAKARAKLGVAGKWVNVKNKEGKKGYVSAELVSEV"
     gene            complement(7900..8391)
                     /locus_tag="PL000_07925"
     CDS             complement(7900..8391)
                     /locus_tag="PL000_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNC88865.1"
                     /translation="MKKPNWILIGILFGVSLFIGFTIFSAAVGAIFPKLHYVSKPLLC
                     SGDFKIETTRYSYKPGQVGWQHNIYCDGREITLLAVFTTGLLFSLGIFMAACIRYWKA
                     LFNPADFGSLAKDLKQADKPARGKKKKSPLERMAELKEMRDQNLISDVEYERKKTEIM
                     NEL"
     gene            complement(8415..9158)
                     /gene="budA"
                     /locus_tag="PL000_07930"
     CDS             complement(8415..9158)
                     /gene="budA"
                     /locus_tag="PL000_07930"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HNC88866.1"
                     /translation="MERTTLRRIRKRKADNKVYLCAPVNSLVEGIYEQNIPFYEIKKH
                     GDFGLGTFNDLDGEMMMVDGEIFRVGSDGRVNQITDDDVCTPFACVTNFQPFSEETLE
                     REVSYVDFLKWMVELMPSPNIFYAFRITGEFGYVKTRSVPKQENYRPLVEVAKEQPTF
                     EFNDVHGTMVGFYTPSFMASLSVPGVHLHFLTDDKSSGGHLLECRPRNVKLELQYLYT
                     LELSLPSNLDYLTWDFQRDIGKDLDKAEK"
     gene            9435..12137
                     /gene="ppc"
                     /locus_tag="PL000_07935"
     CDS             9435..12137
                     /gene="ppc"
                     /locus_tag="PL000_07935"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC88867.1"
                     /translation="MEISQIIHLLGNLLGQVISELESPHIFEIEERIRALAKARRNGD
                     QTSETSLRQEVASLETGEARAVAAAFAAYFDLVNRAEENQRVLILRRYEDETFPEPIR
                     ESIGEAVATLKELGITRGQMEDLLQNLSIELVLTAHPTEARRRTVLSKMERITELITQ
                     ISTGSLSLREQEEATQLLRNEISVLWLTDRARSAKLSVADEVKTGLYFVDSFFWNTIP
                     VIYDDLEKALERNYPGLHPPADWFKLASWIGGDRDGNPNVTAEVTAETLRLHRGLAVE
                     NHRKSFQELARHLSVSSHRIPPPQELMEWIEKRRPFPPHIAYIENRYVTEPYRLALSL
                     LASDLAEASREDMKAHLLQSYAYQARLNTDGLSRPLEMIASAIPFKLALNELQDVHRK
                     LMIFGLQAARLDIREDSSRFNAALGEILRALKIEPEFENLPADSKIELLVRLLKEPAP
                     QLSKHPGATSATAETWSLFQLIGRTHEIYGSDLLGPIIISMTHAASDVLTVLLLAKWA
                     GCKTTPQITPLFESVPDLKDAPRILESLFTLDIYREQLTAHNDEQMVMIGYSDSNKDG
                     GYLMANWSLYEAQEEITRVASEHGIKLTIFHGRGGTIARGGGPANSAIRAQPAGSING
                     RFRLTEQGEIIALRYSNPGLAHRHLEQIASAVILASAPLPHHPISNHWRNAMAQMSEA
                     AQRTYRQMVYETPGFIEFWQSATPLDQIKHLQIGSRPSARSQTGAVNQIRAIPWVFSW
                     MQSRFNLPGWYGLGSGLAMITDRSLLREMYDGWAFFQTLLTNTEMSLLKADMDISALY
                     VDLVPDKALAKEIFSSIRSEYERTREAVLSISRHSTLLELEPATRQAVQLRNPYVDPL
                     NYIQVETLRRLRTLSNPESDEGNALREVMAITINGIASGLRNTG"
     gene            complement(12226..12882)
                     /locus_tag="PL000_07940"
     CDS             complement(12226..12882)
                     /locus_tag="PL000_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNC88868.1"
                     /translation="MIVVSDLMGTLTTGSPFLGLVDWVKHNQSKLSANLYLAAIMPSY
                     LLAKNGLIDWQAWGQKLMVDSLSYIKDADPEKLAHVSEWIVEHDFWKKRREDVVARLI
                     AHREGGAQVYIASSVVEPFIEPFAKRIGAQVSGTPVEIKNGRVQMVGELVASEKKLDQ
                     ILGRLGVKRVDFAYGDTVLDIPLLEHAEHPVAVYPDAKLKSVALERGWEIIGDTPKLG
                     "
     gene            complement(12964..13119)
                     /locus_tag="PL000_07945"
     CDS             complement(12964..13119)
                     /locus_tag="PL000_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC88869.1"
                     /translation="MLFAPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKV
                     NSSLSRV"
     gene            complement(13295..13450)
                     /locus_tag="PL000_07950"
     CDS             complement(13295..13450)
                     /locus_tag="PL000_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNC88870.1"
                     /translation="MLFSPKEKGQGLVEYALILVLVAIVVIAALMILGPLIGNVFSKV
                     NSSLSGV"
     gene            complement(13570..14343)
                     /locus_tag="PL000_07955"
     CDS             complement(13570..14343)
                     /locus_tag="PL000_07955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC88871.1"
                     /translation="MRLKNRTAIITGATSGIGKATAFRFADEGADLVITGRRSGLGKT
                     VEAECRRRGVRCVFVEADHTKQEDCQRVVDVAMKEFGRVDILFNNAGIVPKGTAETTT
                     EEVWQKTLDINITAAWRMSRLVIPHMRAQGKGAIVNNGSDWSVVAGRNVVAYTMSKGA
                     VAMMTKAMALDHAREGIRVNAVCPGDTFVDRWLETGYFEGSDPVTLEEAIKESSSYIP
                     MGRFGKPEEIANAVLFLASDEASFVTGHLLLVDGGNTAQ"
     gene            14403..16817
                     /locus_tag="PL000_07960"
     CDS             14403..16817
                     /locus_tag="PL000_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HNC88872.1"
                     /translation="MDSKTLNVLEYPKILERLAGFCDFSASMDLARTLEPTSSFELAS
                     ARLAETTEARKLLSINSMGVGAAHDIRPQADLAARNGVLDPQALLDVKSTLISCRELK
                     KAFEKRTEEYPRLTQIAMGLPETHGLVDAISRILSDRGEILDSASPKLGDIRRSLRVA
                     HDRLMSRLQKYVTDSKTVSMLQEPIVTQRDGRYVIPLRAEFKGKIKSIIHDQSSSGAT
                     LFIEPLPVVEANNEIRELQLAERDEERRILAEVSRQVGEHATELKYGVENLAVLDLAF
                     AKAKYADELRASEPLMRELKVAGSKSSTFQPSTLKLIEARHPLLDPNTVVPVDVDPAP
                     GTRAIVITGPNTGGKTVSLKTVGLLVVMAQSGLHIPAQSGSELPCFHSVWADIGDEQS
                     IEQSLSTFSGHITNIIRILKKIDSRSLVIFDELGSGTDPQEGAAIARAILTHLLESGA
                     MTLVATHYPELKSFAHSTEGVVNASLEFDIQTLRPTYKLTLGLPGRSNALLIAQKLGL
                     PQTIIESARAEINPLDLRADKLLDDIRKERNRTSREREKLEKARAKLETQTRELEKRL
                     EKIEDERRETLAKARAEGELEVAVLKRNIDSLKGQLKKASQPLQAIKAIEEKMEKMEE
                     KVEAPVERRNEQLSVTGNQAWKLGERVTVSTLNAEGVVTALGESDAEIQIGTLRVRAR
                     LSDLLKGGGQSSSTASKPSPVSIKQKADGGTRTSVAVAKSPGMEVDLRGLMADDALDK
                     MEKYLEQAFLSGLPFVRIIHGKGTGKLRQAVREALRGHEYVSSFEEGGDKEGGEGVTV
                     AKLKSG"
     gene            16894..17136
                     /locus_tag="PL000_07965"
     CDS             16894..17136
                     /locus_tag="PL000_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88873.1"
                     /translation="MSIDDAIKKMTDLIKATCASAEIKSAKMSDEEARLSVYAPAGDM
                     QKIKDATFQPAIDLLNTDGMDVQVFVYDKDAPPLKG"
     gene            complement(17141..17653)
                     /locus_tag="PL000_07970"
     CDS             complement(17141..17653)
                     /locus_tag="PL000_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC88874.1"
                     /translation="MNILAEIHRHENLNIHGRAVCRIAIRGIALHGRKLLMVHSTEVG
                     DYKFPGGGLNAGETHEQALIREVQEECGMSLTCFGAEVGVVVEYLRPQERDCDVFQMT
                     SHYYQCEVDNVFGAQKLDAYEKDLGFKPVWLDVDETIRANKLLLARKSKPGWLKREIL
                     VLEYIRQNLI"
     gene            complement(17751..18875)
                     /locus_tag="PL000_07975"
     CDS             complement(17751..18875)
                     /locus_tag="PL000_07975"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC88875.1"
                     /translation="MNERVKELDGVRGIAIFVVVAFHLLKRADYFTNNEILHFISRLS
                     SIGWVGVDIFFVLSGFLITSILLKTKAEKNYFKNFYIRRALRIFPLYFVFIAIILILI
                     PKLDPDFVPEVPRALPFLLLYQQNWLMLFSKIPLTTYLAVTWSLAIEEQFYLIWPAVV
                     YFTKKETLVKLSIGVILCSILARVLGILFWDDMSAVSTFFYYNTFTRFEQLVLGGLLA
                     VAFTEPFWVQRLKNISVPAFWVAFAAFLALSFTAYPHMVPLDENIWLVLGGYTLVAVF
                     SAALVTILMTHPETSIFRRVFQNKGLIFFGKYSYSIYLLHVPVILILLDVLWRTQLRG
                     WSMYLAYIVLTYAITVVGALLTWNLLEKHMLDLKKYFEYQ"
     gene            complement(18999..21008)
                     /locus_tag="PL000_07980"
     CDS             complement(18999..21008)
                     /locus_tag="PL000_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88876.1"
                     /translation="MMHGPGRAGEFLKNETIKPRNVSETLSRLGGYFGRFWFMLTLAV
                     IFVITATWTQVTSPELIGQATDCFLVPNGSNPFASFAPTEDSDAQTESSCWLASDPAT
                     LTGRRLLLNQLYATGGYTLPDITTATIEQRTEGLVRLIVFIIILFVAGSLLNGMTFFT
                     MSWTGQHVLRQMRVEVFEQLHRLSLSYYAEHEAGDLMSRITNDTTAIEQAFSFALVNV
                     FSGILLLVWVSYNMITANLPFALLSLVVAPLMFIATFYFSEQARKAFRKSREEIGNVN
                     AELQESIAAVREAQAFNRADENIENFKEVNAANRDANVRAVAYTSALAPTLEALSYVS
                     LAIVTGVGGWYLLKGGTFLGSAVTLGLVVTFLAYVQRFNQPIQQIAVLWTNIQNAIAG
                     AERIFTILDEKPAVTDKPNAKEMKEIKGLVEFDKVSHEYEDGVPVLKGVSFTAQPGQT
                     FAIVGPTGAGKTTIINLLPRFYDVTEGSVKIDGVDVRDVAAESIRRQVGIVLQDTFLF
                     SASVMDNVRFGRPDATDEEVMAAIKLANADSFIERLPEKYNTVLGERGSGLSQGQRQL
                     LSIARAALADPRILILDEATSSVDTRTERLIQKAFDKLLSGRTSFVIAHRLSTIRNAD
                     QILVLKAGQIIERGRHDELLEKKGFYYDLYMSQFKKQEEMEAAMA"
     gene            complement(21005..22960)
                     /locus_tag="PL000_07985"
     CDS             complement(21005..22960)
                     /locus_tag="PL000_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC88877.1"
                     /translation="MQQTHVRRIVQKRPERTKSKGGMRRAIKYLTNYKRQAALPYLFL
                     IVATLSQLAVPNMIRRVIDAVTSGYIADQILGALDKIPAQFIGTALPQILDVMKYPAE
                     WTADQLKVQLEADVTNAPQLLTSAIVAILVFAILRGVFAFLQAYWAEKNSQAVAYDLR
                     NDLYSKIQTLSFSYHDKNQTGQLMIRATDDVEKVRLFIGQGLLQLIGAFILLAGTVII
                     LFSTNVSLAWAVMPILPVALVLFIIFAGASRPMFAQVQQKLSALNTVLQENLAGIKVI
                     KAFTRETQQQDKFKARADDTMEQAIKLSRLFTFMFPLVFMIANLGQAAILYVGGKAIM
                     AGTLSLGEWQEFSMYLMYLFMPIMMMGMIITQFGQASASADRIFEILDAKNDIADKPG
                     AVKIPAVKGTVKFEDVTFRYFGGGDPVLSNVTFEAKPGETIALLGATGSGKTTIINLL
                     PRFYDPTEGRITIDGYELRDVTLESLRSQIGIVLQETTLFSGSIRDNIAFGKPDASQE
                     EIEAAAKAAQAHDFIMSFPEGYNTHVGERGTTLSGGQKQRVAIARALLLNPRILILDD
                     STSSVDLNTEAAIQSALDVLMKGRTSFVIAQRISTVMNADKIIVLEKGRVVAIGKHAD
                     LLEDDPIYAEIYNSQILANEHEKEGAL"
     gene            complement(23055..23504)
                     /locus_tag="PL000_07990"
     CDS             complement(23055..23504)
                     /locus_tag="PL000_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC88878.1"
                     /translation="MSKTLPITHPLRTWMDVFMHRSMRGWAHFAKSTGLSMPQFSILM
                     QLHHKGPCGMSTISERFDITAAAASQLVEKLVQGGYLERAEDPNDRRAKLLRLSKEGA
                     KLVSQGMEERYRWMDELVSALNEDEQTQVTEALEILTRAAQSLEDKK"
     gene            complement(23685..24029)
                     /locus_tag="PL000_07995"
     CDS             complement(23685..24029)
                     /locus_tag="PL000_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4377 domain-containing protein"
                     /protein_id="HNC88879.1"
                     /translation="MKKMILIFTLAICFTVFFSACGDGTQVNTREMTLFVGPHQVDCT
                     GGPQGKCFQVKYSAEDSEWVNFGNVIQGFEWEEGYEYELSVKFIETRPKNYDYIIPSF
                     ELIEVLEKTKIE"
     gene            complement(24038..25246)
                     /locus_tag="PL000_08000"
     CDS             complement(24038..25246)
                     /locus_tag="PL000_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC88880.1"
                     /translation="MLEKFFSLRCPDCGGKITEEERYCPHCGVDLEAPISPSIGSNED
                     EHSPARQHFKAAVKGYEKTTKAGLRDALVNCDLALQYDPNFADAHNLRGLVLDEMGRL
                     EEAITAYREALRLNPDLGDARINLEDAVAERANNQSEFGFTNEAIGFAEEEKPRNRGF
                     VKRIPSILALLMIFFVSFLKSDLIKNLANAYLLPKTSVVFIPDVPDGIAVSHADLEEA
                     AQVLSERSQRLGYSNITFEVSTAGEIVGMIPVTMDAVEFSEQVGDVGLLEFVDFGEKP
                     ALAGDIIRTDLESKYLQQVDGHEWHTVMSNEGVLSAASYRSQTDFGFQVSFSLNAEGA
                     QIFLKHTSENVGTYLGIVLDKRVVSVPMINQPITDGQGMISGTFTEEEAKELAVILQT
                     KPLPFPVRLR"
     gene            25446..26843
                     /locus_tag="PL000_08005"
     CDS             25446..26843
                     /locus_tag="PL000_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88881.1"
                     /translation="MSLSEEPIIPTEPTPPSPDPSSLPPRPRRRRATRRDMIPTDAEG
                     QAALISELSRRAYPSIELFVFSLVAGAILGLGFLRDSQAILLLGILITPLMIPWVGFL
                     LASLTGSPRFLFETLMALLISGVIVFIGGLLSGFAARLFLPITLTNVFIHARLWPEEL
                     FVLAIGAITLVASFVRSESKPFLPSVIVAYTFYLPINASGFGLGSGVDGIWPEGILVF
                     ATHFMLASILGLITLLVLRLRPSNRGLILSGVMLLVFTGTLFSLMGNGFPLTTPQTVS
                     TSTPTPSTQPTAIPSLTPSLPPTTTNTVRPSNTPKPTNTPSVDETLSATPTEETENTA
                     TNTSTATKPAATPTKTTSTPAATSTNEPIPTVTMTIEVQTIPGTISADEGGGANLRQT
                     PNGKYLMTLDNNTVVEIYPDFKVVNGITWLHVYVIRNDQQIEGWLLESVVSYATPEPN
                     FEPSSTPEIGITPAP"
     gene            26942..28402
                     /locus_tag="PL000_08010"
     CDS             26942..28402
                     /locus_tag="PL000_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC88882.1"
                     /translation="MPIHFGTDGWRAVISDSFTFDNLRIVTQAIADAVASDHWDKSVN
                     GENGPDKKTIVVGYDTRFLSDRFAGEVARVLAANGFTVLLAQSDSPTPAISFAVKHQR
                     AIAGIMITASHNAPRYNGLKLKGAFGGSALTEQCRRVEVYINDNEEQARGPNLMDFNK
                     ARDAGLIQKFNPLPAYFEHLQKLIRTDIIADNPQRFVVDAMYGSGRGVIKSFLQGTGC
                     EIAEIRGEMNPGFGGVHPEPIAKYLGPLASAVSSGMGNFGVVTDGDADRIGAMDERGN
                     FVDPHKIMALSLKYLVEKRGWSGAVVRTVSTTRMIDRLAKRYGLKLYETPVGFNHIAD
                     YMMKEDVLIGGEESGGISFKGHIPEGDGPIMGLLLVEMIAEAKKSLVEMVDDLLTDVG
                     PALYERVDLRLSRPVAKAEMTEFLTQKAPKEIGGQKVDEISQRDGVKYIMADDSWLLI
                     RPSGTEPVLRVYAEGRNADMVKALMGYGKTVAESVV"
     gene            complement(28522..32247)
                     /locus_tag="PL000_08015"
     CDS             complement(28522..32247)
                     /locus_tag="PL000_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC88883.1"
                     /translation="MKDDNLIPARKIAGIFRDTSRLRRIFYFFIGITSLVLVGGILVV
                     LSTNDVTAALIIGGNLFLMPISTYLVYREKFDVAAGVLAVMLILSNTLLATWGLGIHS
                     INIIAFSTILVMGSLVTRKWMLFSLVILTVLCIGWLVFGELWGLYTPGVLVHSVAGDF
                     FSASILVLTTAILTYKLTEFLFDGFSLLQQEIAERKSVEYDLRERDAIFEAVTFSAEQ
                     FLKSSDWRDSINLVLERLGRTIHASHAYLFEHHNGPDNEMVDSMRYEWTAPGYASDLN
                     NLEFQNSPLNDEGFEDYHQILMNGQPFVGNTSTFQPAERDYFSSLGIKSILEVPLFVN
                     GQWWGTIGFDDYEVERTWNFSELDALKIAGGILSAAIQRQKADSALQESERIYRQAIE
                     AAGAVPYYQDYRQNRYTFIGNGIQRIVGYSPQDFSPDTWNKIVMEAIPLGEGRGQRIE
                     DVVQQVRSGKIRAWQSDVRVTLPNGEERWVNDSAIEMFDDSEVSYASIGILQDVTERK
                     LAEFNLKKHESLLEAVTFAAEQFLKTANWRETIDVVLERLGREFNSSHAYLFQKHAAP
                     NDQVVSSMIHEWTAPGLETDLDTPEFQNMPKNPMGFDRLYKILDSGEPLVGSASFFTT
                     EEKAYLHSMNVAALLEIRIIVNGTHWGTLGVDDIYHEREWTTMEVDVIKVAASVLGAA
                     IERQLTDDALKHELAERRRAEFALRFSEEKFSKAFHTTPVLMTIENSEGRFIDANKSF
                     MDTLGLKREHVIGQSASELGVFQSPQDVHKLRDTFQEKGAIKDLEIGFRRKNGETGVA
                     LLSSERFYVDAGMYTLTSALDITDRKRAEQERERLIADLRLKNAEAETIRETTTIVIS
                     TLDISETVQRILEQLKRVVSYDSASVWLYKGRVAQLMGGIGLPELTEQDKSYIVNETE
                     PDYIFWTQELPYILIGDIQKDYPQFPDFLHGWLSVPLKLRGRLLGFIALDSLTANQFT
                     EADAQMALTYANQVSIALENARLFSDVQNQLNERQKLVNELENKNSELERFTYTVSHD
                     LKSPLVTISGYLGYLEKDALAGKMDRLKIDLQRIQEAVRKMQTLLNELLELSRIGRMV
                     NASQIIPFDELIREAVDIAHGQIQESGATLQTHPNLPAVYGDKPRLVEVMQNLIDNAS
                     KYMGSQPNPQIEIGFRDRQENLLTFFVRDNGIGIEPIHHERIFGLFEKLDAKSEGTGV
                     GLALVRRIVEVHGGKVWVESSPGQGATFYFTLPAADS"
     gene            complement(32308..>34359)
                     /locus_tag="PL000_08020"
     CDS             complement(32308..>34359)
                     /locus_tag="PL000_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC88884.1"
                     /translation="FEGATIGLYRISLKGNILLANTMLARMLGYESVAELLEQDQAGE
                     PIITDYGRKEFLQAIQRDGEMRDFETLWMRSDGTFFSVRENAKLIRDKNGGPLYFEGT
                     VEDITEKKLAEMTLRQNQQRLQAFFNQSLDGFFFCILEKPLPWADAADKEEILLYIFN
                     TQRYTDVNNAMLEQYQITRHHFLSLTSRDIFTHDLQQGLNLRRKLFDEGHLHLETYER
                     KTDGTPVWFEGDYVCMYDERKWITGFFGIQRDITDRKKVEAERETLIQKLAAKNAESE
                     TLRESLAGIVGMFEFLEIVENILDQIRRVIPYDSASVWKWEDNRQKFICGRNLPVTFQ
                     QNEFELDINEDNSALPILLGKVPYVLNNNVQEELKDFRHEPHTYVNSWLAIPLKTRGK
                     IMGLIALDGRSKDQFNEHHTELAVTFANQVAIALENASLFSELQNELGERGKLIKELE
                     LKNAELERFTYTVSHDLKSPLVTINGFLGYLKGDTASGNLDRVRSDIQRIQDAVDKMH
                     LLLRDLLELSRIGRIMNPPETINIEALVNDAREIVHGRLEAQNVTIEIIPPVGTHPGL
                     PAVYGDRQRLTEVLQNLIDNAAKYMGNQPSPRIEIGHQGVENGQLVFFVKDNGMGIDP
                     EHHERIFGLFNKLDAKSEGTGVGLALVKRIIEVHGGRIWVESQAGKGATFFFTLPASP
                     S"
BASE COUNT         8569 a         9377 c         8254 g         7959 t
ORIGIN      
        1 atgtctaaaa tttgcatcgt tccaaaagtg gatgggatcg gcggcatggc atccttccgc
       61 ttgaaatttg agcagggact gcgcgaacgc ggcgtggatg tgacacacga tctttctgag
      121 gattcagacg ctgcgctcgt tattgccggg acccgaaacc tgatcccgtt atggagagcc
      181 cgtcagcgcg gacggcgcgt cgtgcaacgt ctcgacggaa tcaactgggt ccaccgcaaa
      241 aggaacacgg ggctcaaaca ttacctgcgc gccgagtatg gcaaccttgt gttgtcgttc
      301 atccgccgtt ttattgcgac cgatattatt tatcaaagcg aattttcgcg cgattggtgg
      361 gaacgtaaat acggcaagac acgggtgccg ttcgctgtga ttcacaatgg gattgatttg
      421 aatgcgtatt ctgtaaagcg ggataatatc ccgctctacc cgcccttccg ccttttgctc
      481 gtcgaaggca atctcggcgg cggctacgac atgggtctgg acaatgccat cgcactcacc
      541 gaaaccctga tcgaaaaaca caacctgccc gtggaattga tggctgtcgg caaaatatca
      601 gacgatcacc gtcagcgggt ggaagcaaaa acccgtgcgc cgattcaatg gatgggcagc
      661 gtaccgcgtg agcgcatccc tgagatcatg cgctcggcgc atctgttctt ctccgctgac
      721 ttgcaccccg cctgcccgaa ttcggtcatc gaagcactgg cctgcggatt gcccgtcgtg
      781 ggtttcgata caggcgcggt caaggaattg gtcatcggcg atgctggcag ggttgtgccg
      841 tacggaagca actcctggaa catcgaaccg cccgatattt ccgcgctcgc cgttgtcgcc
      901 aaagagattt tgcaaaacca gccgcatttc agccaagctg cgcggactca cgccgaatcc
      961 gcgctggggc tggataaaat ggtggatgca tatttgaaag tcctattaac cacaaagggg
     1021 cacgaaggta cacaaaggtt ttaaaagggt ttcccttcgt gcccctttgt gacacttcgt
     1081 ggttgaatct tttacagagg tcaaatcatg gacaacgaaa ttcacgaatt cctcctcaaa
     1141 cacctgcatg gaatcatgaa caacgacatc gcgtcgtatc atgaaaccac agccgaagac
     1201 ctcaccctgt atgagtggtg ggtcacgccg caccgtctgg acggtcttcc cttccacgaa
     1261 ttcatgatga actccaatgc ctcacgcgga tccgtcttcg gcgcggaaga tcagggacaa
     1321 aaagcgcaaa gtcgctttga cctcgccaac ttgcacattc aacgctacgg cgataccgcc
     1381 attgccagct acacattgct catcagcacc gcgctcccag aaggggtcaa agtggctgca
     1441 cacaacgaaa gccgcgtaat ggttaagatg gacggaaagt ggaaagtagt ccacgtgcat
     1501 aaatcccccg cctactccgc accgcatgtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnt atgatttcct ctggcttggc attgccttgt tcccgctatt gatcattgcc
     1681 gtgctgctgc ccattcaacc gcatgactat tggtggtatt tgcgccttgg gaaagatatc
     1741 ctgcaaacag gttccgtccc cgtcgtggat acgtacagtt ccattcaagc ggggcaaccc
     1801 atcgtctatc aatcctggct ttcggcggtt gtattgtggt gggtgttcaa ggcaggcgga
     1861 gttccattca ccatttttct cgtcgcggtc ttgatcggcc tcacctacgc cctgctctgg
     1921 ttgttgatgc gtaccagcgg aatcggtcca cgcatggcta ccctgctgac gatcatcgca
     1981 gggctggcgg ggagcaacaa ttggggagtc cgcccgcagt tgtttgcata tccgatcttt
     2041 ctcgctgtgt actggcttct gctcaaatgg catcaccgcg aagaaaaatc cctttggctg
     2101 ctcatccccc tcagccttgc atggaccaac ctgcatggtt ccttcatttt gttctttgtc
     2161 ctcgttggca ttgcgtttct cttcggcgct ggcgagcgga aaaaattact ctgggttgcg
     2221 ctggcttcgt ttgcaatctc cttcctcaac ccgcgcggat atgtgctttg gggctccgtg
     2281 atcggcacat tcaccgcccc gggcatccgt gacctcagcc ccgaatggct gccgccgctc
     2341 aaccaaggct ggcagatgaa catctttttc gcatggctga ttctgttgat tccactcgcg
     2401 gccgccagcg ggacccgcaa acgactcacc gcctttgagt ggatcttatt cctcaccttt
     2461 tcatggatgg cactgacagg catccgctat gtgatttggg atctgttcct catcgctgtg
     2521 cttacggcct tcctcatgcc tgaaaccctc atccaaacat ttgaccagcc gcaggaagtg
     2581 aagatcccag ccatgaattt tgcgttgggc gccattttta ttttgcttcc gctcatgctg
     2641 ctccccggcc tgcgcgatgc atggtggcag gaatctccgc ccgcgctgga tcccgtcacc
     2701 acccctgtcg acgccgcaaa atggctggag gagcatgacc agtttccagg tccgatgtgg
     2761 aacgatgtgg tcttcggaag ttatctgatc catgcgcttc cttcacgccc agtgtggatc
     2821 gacacgcgca tccaggtcat cttcaccgcc gagcaggcac atcaatatct tttcgtgcaa
     2881 tcggcgcagc caggttgggg ttcgctgctc aaagagaaaa aagtgaacct gctctttctt
     2941 gccaaaacac aacccgccat tgtggacgcc gtgcaaaatt caagcgagtg gtgcgagcaa
     3001 taccacgatg atgtggcttt tatctttaca cgctgcgagc cgctgccatg aaaaattttc
     3061 cacatcgcct tgggttgcag caacgcgtgc ttcccagtta ccgtgtccca ttttttgatc
     3121 tgctcgcgca atcgtgtgaa agcatgagtc tgtttgcagg ccaggcccgc cccgtggaaa
     3181 tgatcgcggg cggcaggcct caggtcgctg catatcacga agcaaaaaac attcatcttt
     3241 tcagcggcgc gttctattta tgtcatcaac aaaatttcat ttcatggctc gaagaatggc
     3301 atccacacac cttgattgtg gaagcgaacc cccgctatct ggccacatct gccgctgtca
     3361 attggatgca tgcccgtgga agaaaggtca tcggctgggg attgggttcg ccgcctcttt
     3421 ctggacctct ggcagggttc cgtcaaaaaa gcagaatctc gttcctgagc aaattcgacg
     3481 cgctcctcgc gtacagtcaa cgcggcgcag acgaatatgc ggccctcggc ttcccgcgcg
     3541 aaaaaatctt cgtagcacac aactcagttt cgcccaaacc aaccttcaac ctgcaacctg
     3601 caactttcaa acccacgccc tcaattctct tcgtcggccg ccttcaagcc cgcaaacgcg
     3661 ttgaccatct cctccgcgcc tgtgcggaga tggactcaaa accacgattg atgatcgtcg
     3721 gtgacggccc tgaacgcgcc gcattggagt cgcttgcaaa agatgtctac ccttcggcgg
     3781 agttcatcgg cgcaaaacat ggcgctgacc tcaaacccta tttcgaacag gctgatcttt
     3841 ttgtgctgcc gggcaccggc gggctggcag ttcaagaagc gatgagctac gggttgcccg
     3901 tcatcgttgc caaaggggat ggcacgcaag atgaccttgt ccgtgatggg aatggctggc
     3961 agattcaacc cgaagattat ggtgcgctgg tttccacaat gaaaaacgcg ctgtcagaca
     4021 cggcgcgttt gcgaaagatg ggaacagagt cgttccgaat cgtttccgaa gagatcaaca
     4081 tccaaaagat ggtggaagtg tttgtggatg cgttaaacag cgtggcataa aaaatcgcgg
     4141 actgaagtcc gcgatccgat aatcaggggt tcttctcaaa aatataaaca tccgtctctt
     4201 catgaattaa ttgataccca cccgattgtt caagcgaaat caaaagcgaa tttttattcc
     4261 ccgataaacc atcccgcgaa atgaggacat actcaaaggg atggtcttct tttaaagccc
     4321 attcatccac gcaagtggag gttttatcca tgcaggcttg cagggcatag tattcatcca
     4381 gtttcgtcaa aaagattttg tcgggcatcc attcgtaacc ttgaatggtg gcaatgccat
     4441 aactgccgct caacacaggg aaccactccg ccactgcatc ggcaaaggcc tcggtttcgc
     4501 cagtgaggat caaaaaacga ctttgcggcg ggatattccc cgccacccat tccatcgccg
     4561 cacgatctga tgaggtgaga ctcaccttgg ggtacacctg atccgaaagc acagtcccga
     4621 aaaacgcgta gaagatcaat gccgtcaacg caaatttgat cgcagacgat tttataaaat
     4681 acgtgctcca attcgactcg atctcacccg cctcctgcaa attgtgtttc aacgcataca
     4741 acccggggat gacaatatcc acaaagccga tcgccgccag catggcccat ggaatgatcg
     4801 aaatcgaagc cgcactgcgc ggatccacga ccgcaggcag gaagagccac accaccagca
     4861 aaaactcacg ccttgcaatg acgacgacaa accccagcaa agccagtgcg gagagcaccg
     4921 tcacaaattt ttcatcggca aatgaacctg tcagaatcgc cacccacgcc agcggatttt
     4981 gaaacccggt ctgggcggca ctcagataag gctggagtcc gtggcgggag atgacagtga
     5041 gaaaaaatgg cgcggtcagg gcgatcacac ccaaagcgac gagtgaagcg ttcttaatcc
     5101 cctctttgga tcggccataa aacagccaca acaccgcaca cagaaaaacc gtgtgcagcg
     5161 acgcttctgg atggctcaat atcacaccgc ttccaaatag aatcgtcagc accaggtatt
     5221 ttctggaagg cgctgtaaac agtttgtacg cgctgaaaag gatcagcatc agaaagatat
     5281 gccccagcac gcgtgtaatg ccgcccccca tgatgtacca ggagatcgaa cgcggcagca
     5341 gcgcgaaaaa aacagttgcc agactgccct tcaatggaga tttcaaaatc gccgccgcca
     5401 tcgggtagaa cgcaatcgaa aaaagcacac tgcccaccgc gggcatccag cgaaagactt
     5461 caagcagcgg aaagttgaaa atatccgaga tcagggctgc ggtgtaaaac cccaaaggcg
     5521 ggtacgcaaa cgggatcgat ataccgttgt attccacaaa tgacggcagg ttatacccgc
     5581 ccgcctgaat tgccttcacc atttcgtaaa acagcccgcc atccgtcacc ggaaaaccga
     5641 ccatgcttgc gggcaaaaaa cgggcatagg cacccaacaa cagcccgaag accaaaatca
     5701 aaatattcaa gcgttcggtt tctgtcttca agatcacctc ttggtcaaag cggaacgcnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta ttcaagcgtt cggtttctgt
     5881 cttcaagatc acctcttggt caaagcggaa cgcagctcac tgaataaaaa atcaccgcgt
     5941 tacggcttcc ttcacagaaa tcgaaggcgg catgtgtttg aggaatccct cggggtcggc
     6001 ggggcacaaa accacttctt cgatggtttt ggtaaatccg ttcaattttt taggcttgag
     6061 caaaaatccg ttttcgagga tcttgcgctg cggagtgagc acgctccacg ccatcggcag
     6121 ggatttcacc gggccgatcc tttccacatt gcggatctcg tcatagccaa agaacaaacg
     6181 gtaatcaaca tgcccgggga tgtaccgaac cgcaatgccc ttgttcgtca ccagatacac
     6241 ggtgcccgcc ccaaagagca tcgcatcctt gtacaacaac atcacaccga tcagcatcaa
     6301 agtccactgc aaggatgaaa gcccgagctt tccatctgcc acccaaccga taagcgcccc
     6361 tgccgcgcag accagcacaa aattccgcac ctgatacatc acgggcgcat gcggctggtc
     6421 acggtacaaa acacgatact cccgcgcgat ctccgcctcc caggcttttt gctgcatcac
     6481 catgaaccac agcccgagca gaatgatccc tacaaaaatc cccgaaacca caggcggcca
     6541 cgcccacaag gcaatgacaa atacccaggc ataggggatc ttcttcaaaa acaaataagg
     6601 aatggacagg acaaaagaat ggaatgcttt catgtcagca cctacacttc tgaaaccaac
     6661 tcggcgctca cgtatccttt cttgccttcc ttattcttca cgttcaccca tttacccgcc
     6721 acgcccagtt tggcacgcgc ctttgcggca tcttccacca cggtgagttt ggcgcgcatt
     6781 ttttcagtgg atacgatttc cgatttcgct gatgcggtct tgtacacctt tgcacccagc
     6841 ctgcccacac gggccttgac cgtcacgatc atcggtgcag gcttcggcgg tgtggatgta
     6901 gagccgcccg cctcttctgt ggacgttgaa cctgattctg tttcaaccac aggagccgcc
     6961 acttgaacct tttccagata ccatgccgcg caatacccct catgcccttt gccgtcattc
     7021 acccgcaccc actgattgac cgctccgatc ttcgaaatat cgtccgcgcc caacagggtg
     7081 aggagggtac ccgcagaaac aacagcaacg atattgctcg aactggaggt atcaatactt
     7141 gagcggatgt tcaaacccca ggtcaaatct gctttgactc gggccagggt ggtgctggtt
     7201 gtggtcgtac ccgttgtcgg cgtagtttga tccgtttcta tcggttgggt gattccgccg
     7261 ctggcggtga aacattcgta agcaatgaca tgcatgatgg cgcgttccaa tgaattcccc
     7321 tgactgtaac gcggcatgag gtaggtcttt ttggttacat cggtctggta gggccacggg
     7381 tcaagcatca gatagtcatt cccttcacga ccgtagagca cgacccaatg agtgagcagg
     7441 ccgggcgcgg gggtgctatc caccatcacg atgacgggct ggcctgccgc gatggaggca
     7501 tcaataaggc cgagcggtgc atcggtgttg ccgcacgaga tgttgctttt cacattgatc
     7561 tgcggataga tctgattgac cgtatcccag cgaatgcccg cgctgacaaa gccgttgacg
     7621 ttcttcagtt tttggttgag ggtcttgggg gtttcgataa atccatgccc gctgatgagc
     7681 atggccacac tcgtcaacgc gcagccgaca taaccgatcg tgtcacgcga atcgccaaag
     7741 ccaagaatat cttccttcca tttcgggtcc tgctgggagt agtacacaag ctggaatgtc
     7801 atggggcacc tctataaagt tggaatatca cgacagactt attatacagt tcatccgaag
     7861 gagttgtatc accctgaaga agtattttga tctgcctgct cacaactcat tcatgatctc
     7921 ggtctttttt ctttcgtact ccacatcaga gatcagattt tggtcgcgca tttcctttag
     7981 ttcagccatg cgttcaagcg gagatttttt ctttttgccc ctggctggct tgtcggcctg
     8041 tttcaggtcc ttcgccaacg agccgaaatc tgcagggttg aacaacgcct tccaatagcg
     8101 gatgcaggcc gccatgaaaa ttcccagcga gaagagcagg ccggtcgtaa aaacggccag
     8161 cagggtgatc tccctgccat cgcagtagat attgtgctgc cagcccacct gtccgggctt
     8221 gtaggaataa cgtgtcgttt cgatcttgaa atctcccgaa cacagcagcg gcttggaaac
     8281 ataatgcagt tttggaaaga tcgccccgac agccgcagaa aaaatggtga acccgatgaa
     8341 caggctgacg ccaaacaaaa ttccgatcaa gatccagttc ggttttttca ttatttttct
     8401 ccgtagcggt aaaactattt ctcggcctta tccaggtcct tgccgatatc gcgctggaaa
     8461 tcccaggtga gataatcgag gttggacggc agtgacaatt ccagcgtgta caggtattgc
     8521 aattccaact tcacattgcg cggacggcat tccagcaaat gtccgccgct ggatttatcg
     8581 tcggtgagaa aatgcagatg cacaccgggc acgctcaggg aagccatgaa cgagggtgtg
     8641 taaaatccga ccatcgtgcc atgcacatcg ttaaattcaa aagtcggctg ttccttcgcc
     8701 acttccacca gcggacggta attttcctgt ttcggcaccg agcgtgtttt gacgtaccca
     8761 aattcacccg tgatgcgaaa cgcataaaag atattgggtg aaggcatcaa ctccaccatc
     8821 cattttagaa aatccacata ggaaacctcg cgttcgaggg tctcttcact gaaaggttga
     8881 aaattggtca cacaagcgaa gggcgtacaa acatcatcat ctgtgatctg attcaccctg
     8941 ccatcagacc caacgcggaa aatctcaccg tccaccatca tcatctcgcc atcgagatcg
     9001 ttgaaagtgc cgaggccaaa atcgccgtgt tttttgatct cgtaaaaagg gatgttctgt
     9061 tcgtagatgc cttccaccag ggaattgacc ggcgcgcaca aatacacctt gttatcggct
     9121 tttcgttttc taattctccg cagggttgtc cgttccattt tgcagtctcc taaaatgtga
     9181 tgaaggcgat tatacacttg gtacaaatca acgtcagggg agtggctaaa aggatgggct
     9241 aacgactctt ccaccacaga gatcacggag ttcacagaga ttttcaaaag gttttctccg
     9301 tgttctctgt gcgctctgtg gttaaatctt tggttggcaa gcccacgaaa ttagccactc
     9361 cccgcttcag agattcggca aattaaaagt ggcggaagtt ataatcggcg aagcatttca
     9421 aaagaggaaa gcccatggaa atctctcaaa tcattcacct gctcggcaat cttctgggac
     9481 aggtcatttc agagcttgag tcgccgcaca tctttgaaat cgaagaacgc atccgcgcat
     9541 tggcaaaagc ccgccgcaac ggcgaccaga cctccgaaac aagcctgagg caggaagttg
     9601 cttcgctcga aacaggggaa gcgcgggcgg tcgccgctgc gttcgccgcc tactttgatc
     9661 tggtcaatcg cgcggaagag aatcagcgcg tgttgatcct gcgccgttac gaagacgaaa
     9721 cctttccaga gccgatccgc gaatccatcg gcgaggccgt tgccaccctc aaagaactcg
     9781 gcatcacccg cggacaaatg gaagacctgc tccaaaatct atccattgaa ctcgtcctca
     9841 ccgcccaccc caccgaagca cgccgccgta ctgtgctctc caaaatggaa cgcatcacgg
     9901 aactcatcac ccagatcagc acaggctcac tttccctgcg cgaacaggag gaagccaccc
     9961 aactcctgcg caatgaaata tccgttttat ggcttaccga ccgtgcccgc tctgccaaac
    10021 tttccgtagc cgatgaagtg aagaccgggt tgtactttgt cgattctttt ttctggaaca
    10081 ccatccccgt catttacgat gaccttgaaa aggcgctcga acgaaactac cccggcctgc
    10141 acccgcccgc cgactggttc aaactggcct catggatcgg cggcgaccgc gatgggaatc
    10201 ccaacgtcac cgctgaagtg accgccgaaa ccctgcgcct gcatcgcggc ctggcggttg
    10261 aaaatcaccg caagagtttt caggaactgg cccgccattt aagtgtaagc tcacaccgca
    10321 ttccaccgcc tcaggaatta atggagtgga tcgaaaagcg cagaccattt ccacctcaca
    10381 tcgcctacat cgaaaatcgg tatgtcacgg aaccctaccg tctggccctg tctctgctgg
    10441 cttccgacct cgccgaagcc tcacgcgaag acatgaaagc acacctgttg caaagttatg
    10501 catatcaagc ccgcctcaac accgatggtc tctcgcgccc gttggagatg attgcctcgg
    10561 ccattccatt caaactggcc ctgaacgaat tgcaggacgt ccaccgcaaa ttaatgatct
    10621 tcggcctgca ggccgcacgg ctcgacatcc gcgaagactc aagccgcttc aatgccgcac
    10681 tcggcgaaat tctgcgtgcg ctgaagatcg aacccgaatt tgaaaacctg cccgccgatt
    10741 caaagatcga attactggtg cgcctgttaa aggaacctgc tccgcagctt tccaaacatc
    10801 ccggggcaac ctcggccacc gctgaaacct ggtcactgtt tcaacttatc gggcgcactc
    10861 atgaaatcta cggaagtgac ctgctcggcc ccatcatcat ctcaatgacg cacgccgcat
    10921 cagatgtgct caccgttctc ctgttggcaa aatgggcagg ctgtaaaacg accccacaga
    10981 tcacaccgct cttcgaatcc gtgccagacc tgaaagacgc gccgcgcatt ctcgaatcac
    11041 tttttacctt ggatatctac cgcgaacagc tcaccgccca caacgacgaa caaatggtga
    11101 tgatcggcta ctccgacagc aacaaagacg gcggttatct catggcaaac tggtcgcttt
    11161 acgaagcaca ggaagaaatc acccgcgtgg caagcgaaca cggcatcaaa ctcaccatct
    11221 ttcatggccg cggcggaacc attgcgcgcg gaggcggacc cgccaacagc gccatccgtg
    11281 ctcaaccggc aggcagtatc aatggtcggt ttcgcttgac cgaacagggc gagatcattg
    11341 ccctgcgcta ttccaatccc ggcctggcac atcgtcatct tgaacagatc gccagcgccg
    11401 tcatccttgc ttctgcgccg ctgccgcatc accccatttc aaatcactgg cgcaacgcca
    11461 tggcacaaat gtcagaggcg gcacaacgca cctaccgcca aatggtttat gaaaccccgg
    11521 gcttcattga attctggcaa tcggccacac cgctcgacca gatcaaacat ctccagatcg
    11581 gctcacgccc atcggcacgt tcgcaaaccg gtgcggtcaa tcagatccgc gccattccat
    11641 gggtcttctc gtggatgcaa agccgcttca acctgccggg ctggtatggg cttggttcag
    11701 ggctggccat gatcaccgat cgttcgctct tgcgtgaaat gtacgatggc tgggccttct
    11761 tccaaaccct gctcaccaac accgaaatgt ccctgcttaa agcggatatg gatatctccg
    11821 ccttgtacgt ggacctggtt ccagacaaag cgctggcgaa ggaaatcttc tcttcgatcc
    11881 gcagcgaata tgaacgtacg cgggaagctg ttctttccat cagtagacat tccacactgc
    11941 tggagcttga acccgccacc cgccaggccg tgcagctgcg caacccctat gtggacccgc
    12001 tcaactacat ccaagtggaa accctgcgcc gcctgcgtac actctccaac cccgaaagcg
    12061 acgaaggcaa tgccctgcgt gaagtcatgg cgatcaccat taatgggatc gcgtcagggt
    12121 taagaaatac agggtgagcc tatcgaataa tagaaaataa aaaggtcttg aagaaatgta
    12181 aaacttcttc aagaccttca actcttaaac ctgtaacttt caactctaac caagctttgg
    12241 cgtatcgcca atgatctccc aaccccgctc cagcgcaaca gatttcaact tcgcatcggg
    12301 gtagaccgcc acggggtgtt cagcatgttc aagcagggga atatccagca cggtatcgcc
    12361 ataggcaaaa tccacacgct ttacaccgag gcggcccaga atctgatcca gttttttctc
    12421 gcttgcaacc aattcaccca ccatttgaac acggccattc tttatttcca caggcgtacc
    12481 actgacctgc gccccgatcc gttttgcgaa cggttcaatg aatggctcca ccacgctgct
    12541 ggcaatatac acctgtgctc cgccttctcg atgagcgatc aaccgtgcca ccacatcctc
    12601 gcggcgcttc ttccagaaat catgttccac gatccactcg gatacatggg ccagtttttc
    12661 agggtctgca tccttgatat aagacaggct gtccaccatc aacttttgtc cccaggcctg
    12721 ccagtcaatt aatccatttt ttgcaagcag ataagacggc atgatcgccg ccagatacag
    12781 attggcgctc aacttgcttt ggttatgctt tacccaatcc accaacccta aaaacggtga
    12841 ccctgtggtc aatgtgccca ttaaatctga tacaacgatc atggtttcct ctacgcaatg
    12901 catgggcggc tcgttgggaa caacgggccg cccatgcatg attctgacat tctctgtctg
    12961 ctactacaca cgagacaggc tgctatttac tttactaaat acattaccga tgatcgggcc
    13021 caatatcata agcgcagcaa taacaacgat ggctactaaa acaagaatta gcgcatattc
    13081 gaccagacct tgacccttct ctttcggtgc gaataacata tttttttacc tcctttctta
    13141 aaaattcaaa tacggcactg ttattataat taaaaagctg gtcagtttag ttaacaaatc
    13201 agttagttca gtaatttggt gaaaaacaat gggcggccca ttgcatgcaa cgggccgccc
    13261 aaatacggac ttctttgctt cctatctgat gcaattacac accagacaag ctgctgttga
    13321 ccttgctgaa tacatttccg atcaatggtc caagaatcat aagcgcagcg ataacgacga
    13381 tggcaaccaa aacaagaatc agcgcatact caaccaaacc ttgacctttc tctttcggtg
    13441 agaataacat ggtgactacc tcctttcctt tggtatggta cagcaatttc attatagcca
    13501 gaaattatcc ggaatcaacc tacaaatctg ttgttattgg ccccatttgg gctattttgc
    13561 acaaaacatt tattgcgctg tattcccgcc atccaccaaa agcaggtgcc ccgttacaaa
    13621 agatgcctca tctgaagcca gaaaaagaac agcattggca atctcttcag gctttccaaa
    13681 acgccccatc ggaatgtatg aacttgattc tttgatggct tcttccaatg tcaccgggtc
    13741 ggaaccttca aagtaaccgg tctccagcca tcggtccaca aaggtatcgc cggggcaaac
    13801 tgcattcaca cggattccct cacgggcatg atcgagagcc attgccttgg tcatcatcgc
    13861 cacggcacct ttgctcatgg tgtaggccac tacattcctg cctgccacta ccgaccaatc
    13921 tgaaccgttg ttgacgattg cccccttccc ctgtgcgcgc atatgcggaa tgacaagcct
    13981 gctcatccgc catgccgcgg tgatgttgat atcgagggtc ttttgccaga cctcttcggt
    14041 ggttgtctcc gctgtaccct tcggcacaat gccagcatta ttgaacaaaa tatcaactcg
    14101 accaaattcc ttcatggcga catccaccac ccgttgacaa tcttcctgtt tcgtatgatc
    14161 tgcctcaacg aacacgcatc ggactccccg ccgcctgcat tcagcctcga cggtttttcc
    14221 caacccggag cgtctgcctg ttatgaccag atcagccccc tcgtctgcga aacgaaacgc
    14281 ggtggctttt ccaattccgc tggtggcgcc tgtgatgatc gctgttctat ttttgagtct
    14341 catcccgttc tccattcttc accatgctgt attcatcgtt tcaagaagta taattcatca
    14401 ttatggacag caagacactc aacgtactcg aatacccaaa gatcctcgaa cggctggcag
    14461 ggttctgcga tttttccgct tcgatggacc tggcacgcac actcgaacca acctcttcct
    14521 tcgaactcgc ctctgcccgt ctggcagaga ccaccgaagc gcgcaaatta ctttccatca
    14581 acagcatggg tgtgggggct gcgcatgata tccgcccgca agcggacctg gctgcacgca
    14641 acggcgtcct tgacccgcaa gccctgctgg atgtcaaatc cacgctgatc tcgtgccgcg
    14701 aattaaaaaa ggcgttcgaa aaacggacag aggaataccc gcgcctgact cagatcgcca
    14761 tggggctgcc tgaaacccat ggcctcgtgg atgcgatttc gcgcatcctt tccgaccgcg
    14821 gcgaaattct ggactcagcc tcgccaaaac ttggcgacat tcgccgcagt ttgcgtgtgg
    14881 cacatgatcg cttaatgagc cgactgcaaa aatatgtgac cgattcgaaa accgtttcga
    14941 tgctgcagga gccgatcgtc acccagcgcg atgggcggta cgtgattcca ttgcgggcgg
    15001 agttcaaggg caagatcaaa tccatcatcc atgaccagtc ttcgagtgga gccacgcttt
    15061 tcattgaacc gttgccggtg gtggaggcca ataatgagat ccgcgaactg caactggccg
    15121 agagagatga agaacgcaga atccttgccg aggtttccag gcaggtcggt gaacacgcca
    15181 cagagttgaa gtatggtgtg gagaacctcg ccgtgcttga ccttgcgttt gcaaaagcaa
    15241 aatacgctga tgagctccgc gcaagtgaac ccttgatgcg tgagttgaag gttgctggtt
    15301 caaaatcttc aacttttcaa ccttccactt taaagcttat tgaagcccgt cacccattgc
    15361 ttgatccaaa taccgtcgtc cctgtggatg tggaccctgc ccccggcaca cgagccattg
    15421 tcatcacggg gcccaacaca ggcggcaaaa cggtctcgtt gaaaacggtt gggcttttgg
    15481 tggtgatggc gcaaagcggg ctgcacatcc ccgcacaaag cggatctgaa ctaccgtgct
    15541 tccattcggt gtgggctgat attggcgatg agcaatccat tgaacagtca ctctccacgt
    15601 tcagcggaca catcaccaac attatccgta tcctaaaaaa aatagacagc cgttcactcg
    15661 tcattttcga tgaacttggc tcaggcaccg acccgcagga aggcgcagcc attgcgcgtg
    15721 cgatcctaac ccacctgctg gaatcgggtg caatgaccct cgttgcaaca cattaccccg
    15781 agttgaaatc ctttgcgcat tcgaccgaag gagtggtgaa cgcttctctg gaatttgaca
    15841 ttcaaacgct tcgccccacc tacaaactga ctctcggtct cccgggcagg tccaacgcct
    15901 tgttgatcgc tcaaaagctg ggattgccgc aaaccatcat cgaatcagca cgggctgaga
    15961 tcaatccgct ggatctgcgg gccgataaac tgctggatga tattcgcaag gaacgtaacc
    16021 gcacttcacg cgagcgcgag aagctcgaaa aggcacgcgc caaattggaa acccaaacac
    16081 gtgaattgga aaaacgcctt gagaagatcg aagacgaacg ccgcgaaaca ctcgccaaag
    16141 cccgcgcgga aggcgagttg gaagtggcgg tgctcaagcg caacattgac tcgttgaagg
    16201 gtcaattgaa aaaagccagc cagcccctgc aggccatcaa agccattgaa gaaaaaatgg
    16261 aaaagatgga agaaaaggtg gaagcgcctg tggagcgaag gaatgagcag ttatccgtca
    16321 caggcaatca ggcatggaag ttgggtgaac gggtcactgt cagcactttg aatgccgaag
    16381 gtgttgtaac cgctttgggt gagtcggatg cggagatcca gatcgggact ctgcgcgtgc
    16441 gggcgaggct atctgacctg ctgaaaggcg gcgggcagtc atcatcaaca gccagcaagc
    16501 catcccctgt gagcatcaag cagaaggctg acggcggcac gaggacttcg gtggcggttg
    16561 caaaatcgcc gggcatggaa gttgacctgc gcggattgat ggccgatgat gcactcgata
    16621 aaatggaaaa atatctggaa caggcttttc tttcaggact gccttttgtg cgcatcattc
    16681 acggaaaagg gacgggcaaa ctgcgtcagg ctgtacgcga ggccctgcgc gggcacgagt
    16741 atgtaagctc gttcgaagaa ggcggcgata aggaaggcgg cgaaggcgtg accgttgcaa
    16801 agttgaaaag cgggtaggaa attggtcagg tctgcggtgt aacctgctgc aggatggtat
    16861 ataatttttt taaatttaaa cagggagctt tccatgtcca ttgatgatgc cataaaaaaa
    16921 atgacagacc tgatcaaggc cacatgcgca agtgcggaga ttaaatccgc aaagatgtct
    16981 gatgaagaag cgcggctttc agtgtatgcg cctgcgggtg atatgcaaaa gatcaaggat
    17041 gcgacgtttc aaccagccat tgacctgctc aacaccgatg gaatggacgt gcaggttttt
    17101 gtgtacgaca aggatgcccc gccgctgaag ggttgatgtt tcagatcagg ttttgcctga
    17161 tatattcgag aacaagaatt tcccttttca accatccggg tttactttta cgagccagca
    17221 ataatttgtt ggctcgtatt gtttcatcca catcaagcca cacgggtttg aaacccaggt
    17281 ctttttcgta tgcatcgagt ttctgggcgc caaaaacgtt atccacttcg cattggtaat
    17341 agtgtgaggt catttgaaat acgtcgcaat cccgctcctg tggacgaaga tactcaacca
    17401 caacgccaac ctctgcgcca aaacaagtca gtgacatgcc gcattcttcc tgtacttcgc
    17461 ggatcagggc ctgttcatgc gtttcacctg cgttcagtcc gccgccgggg aatttataat
    17521 cgccgacctc agtggagtga accatgagca atttccgtcc atgcaaggct atgccgcgga
    17581 tggcgattcg acacactgcc ctgccatgga tgttgagatt ttcatgtcgg tggatttcag
    17641 caagaatatt cattgaactc cctcaatctt tctatggcga ttggtcaaat gcatgcagtt
    17701 tgcaaagata tttccctgaa actttgacat caaaaagaca acaatttcgc ttattgatat
    17761 tcaaaatact ttttcaggtc gagcatgtgt ttttcaagca ggttccatgt gagcaaggcg
    17821 ccgacgacag tgatggcata ggtcaaaacg atgtaagcaa ggtacatgct ccagccgcgc
    17881 aattgggtgc gccagaggac atccaataaa atcaggatga caggcacatg caaaaggtaa
    17941 atggagtaac tgtattttcc aaagaaaata agacctttat tctgaaatac cctgcggaag
    18001 atggacgttt cagggtgggt catcaaaatg gtgaccaatg ccgctgaaaa aaccgccacc
    18061 aatgtatagc ctcccaaaac cagccagata ttttcatcca gtggaaccat gtgggggtag
    18121 gctgtaaaac tcaaggccag gaacgccgca aacgcaaccc aaaaagctgg gacagagatg
    18181 tttttcaggc gttgcaccca aaacggctcg gtaaatgcaa ctgcaagcaa cccgcccaaa
    18241 accaactgct caaaacgggt aaaagtattg taataaaaga aggttgaaac agccgacatg
    18301 tcatcccaga acaaaattcc caacacacga gcaaggatcg aacagaggat caccccgatg
    18361 cttagtttga ccagcgtctc ctttttcgta aaataaacga cggcaggcca gatcaaatag
    18421 aactgctctt cgatggccag tgaccaggta accgccagat aggtggtcaa cgggatcttg
    18481 ctgaacagca tcagccagtt ttgctgatac agcagcagga agggcagggc gcgtggaact
    18541 tcaggcacaa aatcggggtc aagttttgga atcaggatca gaatgatggc aatgaaaaca
    18601 aagtacaggg gaaagatccg caaggcacgg cggatgtaaa aattcttgaa ataatttttt
    18661 tcagcctttg tcttcagcaa aatggatgtg atcaaaaacc ccgagagcac aaaaaaaata
    18721 tccacgccta cccagccgat ggaagagaga cgtgaaatga aatgcaggat ctcattattg
    18781 gtgaaataat cggcgcgctt aagcaaatgg aacgccacaa cgacaaaaat cgcaattccc
    18841 cgcacgccat ccaactcctt aacccgctca ttcataaaac gaaatatcca ttttctgtgc
    18901 tggcttcgag ttcagatggg gcatggcgct tctcctaaac ccccatttaa ccacaaaaac
    18961 agcagtgaca cggcgctacc gtgtcactgc tacaaaagtt acgccattgc ggcttccatc
    19021 tcttcctgtt ttttgaactg gctcatgtac aggtcgtagt agaagccttt cttttcaagc
    19081 agttcatcat gcctgccgcg ctcaatgatc tgcccagctt tcaacaccag gatctggtct
    19141 gcattgcgga tggtggaaag acgatgcgcg atgacaaagg aggtgcgtcc gcttaaaagt
    19201 ttatcgaatg ccttttggat gagacgctcg gttctcgtat ccacactgga agttgcttca
    19261 tccagaatga gaattcgcgg gtctgccaga gccgcacgcg caatggacaa caactggcgc
    19321 tgtccctggc tcagccctga accacgttct cccaaaacag tattgtattt ttcgggcagg
    19381 cgttcaatga acgaatcagc attggcaagt ttgatcgctg ccatcacctc ttcatcggtg
    19441 gcatcgggtc gtccaaagcg gacgttatcc atgaccgaag cggagaagag gaaggtatct
    19501 tgaagcacga tgccaacctg cctgcgaatg gattcagcag cgacgtcgcg cacatccacg
    19561 ccatcaattt ttaccgaacc ttcggtcaca tcatagaaac gcggaagcag gttgatgatg
    19621 gtggtcttgc ccgctcctgt ggggccgaca atggcaaagg tctgcccggg ctgggctgta
    19681 aatgaaacac ccttcaacac gggcacgcca tcttcatatt catgggagac cttgtcaaac
    19741 tcaacgaggc ctttgatctc cttcatttcc tttgcgttgg gtttatccgt cacagcaggt
    19801 ttttcatcga gaatggtgaa gatacgctcc gcaccagcga tggcattttg aatgttcgtc
    19861 cacaacacag cgatctgctg gatcggctgg ttaaagcgct ggacataagc aaggaaggtg
    19921 accaccaagc caagggttac agcagaaccg aggaaggttc cgcctttgag cagataccac
    19981 ccgcccacac cagtgacgat tgccaacgac acataagaca gggcttccag ggtcggggcc
    20041 agggccgagg tgtaagccac agcacggacg tttgcatcgc ggttggcggc gttgacctct
    20101 ttgaagtttt caatgttctc atctgcacgg ttgaaagcct gggcttcccg taccgcagca
    20161 atggattcct gtaactccgc gttgacatta ccgatctctt cgcgtgattt tctaaaagcc
    20221 ttgcgggcct gctcggagaa gtaaaacgtg gcgataaaca tcaacggcgc aacaaccagt
    20281 gaaagcaggg caaacggtaa attggccgtg atcatgttgt aggaaaccca caccagcagc
    20341 agaatgccgc tgaacacatt gaccagcgcg aacgaaaaag cctgttcaat ggcggttgta
    20401 tcgttggtga tgcggctcat caagtcacca gcttcatgct cggcataata gctgagcgaa
    20461 aggcgatgca gttgttcgaa cacctccaca cgcatctgac gcagcacatg ttgacccgtc
    20521 cacgacatgg tgaagaaggt cataccgttc aaaagggaac ccgccacaaa gagaatgatg
    20581 atgaacacga tcaaacggac caatccttcg gtgcgctgtt caattgtggc agtggtgata
    20641 tcaggcaggg tgtaaccacc cgtggcgtag agctgattaa gaagcaggcg tctgcctgta
    20701 agggtggcgg ggtctgaggc aagccaacag gaagattctg tttgggcatc agaatcctct
    20761 gtcggtgcga atgaagcgaa cgggttgctt ccattcggga cgaggaaaca atccgttgcc
    20821 tgtccgatca attcagggct ggttacttgc gtccaggtgg ctgtgatgac aaagatcacg
    20881 gccagagtca gcatgaacca gaagcggcca aagtatccgc caagacgtga aagggtttcg
    20941 ctcacgttgc ggggtttgat ggtttcgttt ttaagaaatt cgcctgcgcg gcctggtccg
    21001 tgcatcataa tgcaccttcc ttttcatgtt catttgccaa gatctgagag ttataaattt
    21061 ctgcgtaaat ggggtcatct tccagcaggt cggcatgttt gccaattgca accaccctgc
    21121 ccttttcgag cacgatgatc ttgtcggcat tcatcactgt gctgatgcgt tgcgcgatga
    21181 caaaggaagt gcgtcccttc ataagcacat ccagcgcaga ttggatcgca gcttcggtgt
    21241 tgaggtcaac acttgaagtg gaatcgtcga ggatgagaat gcgcgggttg agaagcaggg
    21301 cacgggcaat ggcgacgcgc tgcttttgtc cgccagacaa ggtggtaccg cgctcgccga
    21361 catgtgtgtt atagccttca gggaacgaca tgataaagtc gtgcgcctgt gctgcttttg
    21421 cagcggcttc gatctcttcc tgtgaagcat cgggtttacc aaaggcgatg ttatcgcgga
    21481 tgcttccgct gaacagggtg gtttcctgca acacaatacc gatctgcgaa cgcagtgact
    21541 cgagggtcac atcgcgcagt tcataaccat caatggtgat gcggccttct gtggggtcat
    21601 agaaacgcgg cagcaggttg atgatggtgg tcttgcctga acctgtggcg ccaagcaggg
    21661 caatggtctc gcccggtttg gcttcaaaag tgacattgga aagcacaggg tcgccgccgc
    21721 caaaataacg gaaggtgaca tcttcgaatt tcacggtgcc cttcacagca gggatcttga
    21781 ccgcgccggg cttatcggca atgtcattct ttgcatcgag gatctcgaag atgcggtcgg
    21841 cggatgcaga agcctgaccg aattgagtga tgatcatgcc catcatcatg atcggcataa
    21901 agagatacat caaatacatc gaaaattcct gccactcgcc aaggctcaac gtaccagcca
    21961 tgatggcctt gccgcccaca tacaaaatgg cggcctgccc cagattggcg atcataaaca
    22021 ccagcgggaa cataaacgtg aacaagcgtg aaagtttgat ggcctgttcc attgtgtcat
    22081 cggcacgcgc tttgaactta tcctgctgct gggtttcacg cgtaaaagct ttaatgacct
    22141 tgatacctgc aaggttctcc tgcaacaccg tgttcaaggc agaaagtttc tgctgcacct
    22201 gtgcgaacat cgggcggctt gcgcccgcaa aaatgatgaa caataccaat gccacaggca
    22261 aaatcggcat cacagcccat gccagtgaaa cgttcgtcga aaacaaaatg atcaccgtac
    22321 ccgccagcag gatgaatgcg ccgatcaact gaagcaagcc ctggccgata aagaggcgca
    22381 ccttttccac atcatccgtg gcgcggatca tcaattggcc tgtctgattc ttgtcatgat
    22441 acgagaatga cagggtctgg atcttggaat acaggtcatt gcgcagatcg taagccaccg
    22501 cctgcgaatt tttctccgcc caatatgcct gcaaaaatgc gaagacgccg cgcaagatcg
    22561 caaaaacgag aatggcgaca atggcactgg tcagaagttg tggggcattg gtaacatccg
    22621 cttccaactg gaccttgagc tggtctgccg tccattcagc aggatacttc atcacatcca
    22681 gaatctgcgg cagggctgtg ccaataaatt gcgcggggat cttatccagc gcgccaagga
    22741 tttggtctgc aatgtaaccg ctggtcaccg catcaatgac acgtcggatc atattcggca
    22801 ctgccaattg tgacagtgtg gccacgatca ggaataaata tggcaacgct gcctgacgct
    22861 tgtaattcgt cagatattta attgcgcgcc tcatgccgcc tttggacttg gtccgctcgg
    22921 ggcgtttttg aacgattctt cttacatgag tttgttgcat gttgctccta ttaataattg
    22981 gagattggta attgggacta tgaatatcaa aaaaatgtgc agaaactatg gaaaagaaag
    23041 ccagaaattg cggatcattt cttatcctcc aaagactgcg cggcacgggt caaaatctcc
    23101 aacgcttcgg tgacctgggt ctgctcatct tcgttcaacg cagagaccaa ttcgtccatc
    23161 caccgatagc gctcttccat accctggctg accagttttg cgccctcttt tgagagacgc
    23221 agcaattttg cacgacggtc atttggatct tcagcccgtt caaggtagcc cccctgaacc
    23281 agtttttcca caagctggct ggcggccgcg gcggtgatat cgaaccgttc gctgatcgtc
    23341 gacatcccac aaggcccttt gtggtgcaat tgcatcagaa tgctgaattg cggcatcgaa
    23401 agcccggtgg attttgcaaa atgagcccat ccgcgcatgg aacgatgcat gaatacatcc
    23461 atccatgtgc gcaaggggtg ggtaattggg agggttttgg acatagttaa gccttctttt
    23521 tagttaagcg gactaaacta tatccccgta tgaaccttgg gtcaagggca gaagagttag
    23581 agaagcgtta gaaaatttgg aatcgtccaa acaaggaaaa tctcggcgat atgccctatg
    23641 gacacatcgc cgagattttg aaaaggtgca agaggttgat ttttttactc gattttcgtt
    23701 ttctccagca cctcgatcaa ctcaaaggac ggaatgatat agtcataatt cttcgggcgg
    23761 gtttcgatga atttgacgct caattcatac tcatatccct cctcccattc gaaaccctgg
    23821 atgacattgc caaaattcac ccattcggaa tcctcggcgg aatacttgac ctgaaagcac
    23881 ttcccctgtg gaccacccgt acaatccacc tggtgcggac caacgaacag ggtcatctca
    23941 cgggtgttga cctgagtacc gtcaccgcaa gctgaaaaaa agacagtgaa acaaatggcc
    24001 agtgtaaaaa tcaagatcat ctttttcatg gataccttta tcgaagccgc acagggaaag
    24061 gcagcggttt cgtctgtaat atgaccgcca actctttcgc ctcttcttca gtgaaagttc
    24121 ccgaaatcat cccctgtcca tcggtaattg gctggttgat catcggaaca gagaccaccc
    24181 gtttatccaa aacgattccg agataggtac caacattttc ggaagtgtgt ttcaaaaaaa
    24241 tttgcgcccc ttccgcgtta agtgaaaaac tgacctgaaa accaaaatca gtttgagaac
    24301 gatacgatgc agccgacaga acaccctcgt tcgacatgac cgtatgccat tcatgcccat
    24361 ccacctgctg aagatactta ctctccaaat ctgtgcggat aatatcccca gccaatgcag
    24421 gtttctcacc gaaatccacg aactccaaaa ggcccacatc ccccacctgc tctgaaaact
    24481 ccacagcatc catagtgaca gggatcatcc ccacgatctc acctgccgtg gaaacctcaa
    24541 atgtgatgtt ggaatatcca agccgctggc tgcgttctga aagaacctgt gcagcttctt
    24601 caaggtctgc atggctcaca gcaatgccat caggcacatc ggggataaac acgaccgaag
    24661 tctttggcag cagataagca ttggctaaat ttttgatcag gtcgctcttt aaaaatgaaa
    24721 caaaaaagat catcaacaaa gccagaatgg agggaatacg ttttacaaag ccgcggtttc
    24781 gtggtttctc ttcttcagca aaaccaatgg cctcattcgt aaatccaaac tcagactgat
    24841 tattagcccg ttccgccact gcatcttcaa gattgatgcg cgcatcacca agatcaggat
    24901 tcaaccgcaa tgcttcgcgg taggccgtaa tggcttcttc gagccgtccc atttcatcaa
    24961 ggaccagtcc gcgcaaatta tgcgcatcgg caaaattcgg gtcatattga agcgccagat
    25021 cacaattgac gagcgcatca cgtaaacccg ccttcgtggt cttttcatac cccttcaccg
    25081 cagccttaaa atgctgacgg gcggggctat gctcatcctc gtttgaacct atggaaggtg
    25141 agatcggcgc ttccaagtcc accccgcaat gcggacaata ccgctcctcc tctgttatct
    25201 tcccgccaca atcgggacaa cgcaaactga aaaacttttc caacatggca gccatccttg
    25261 aaataaattt acccgcctta actttatgat tctgaaaaca tttcgtcaag ggattgggct
    25321 ggttctttgg taccttccat ataattacat gacaaatcaa ttcccacctc ccactctcca
    25381 ttcatcccct gtataatgga acatcatgat tctcccgacc atttcagggt acatgtagaa
    25441 acgccatgtc tttatctgaa gaacccatca tccccaccga accaaccccg ccctcaccag
    25501 acccgagcag ccttccgccg cgcccgcgcc gcagacgcgc aacccgccgc gatatgatcc
    25561 ccaccgatgc cgagggacag gccgcgctga tctccgaatt atcgcgccgc gcctatccat
    25621 ccattgaatt atttgtgttt tcattggtgg ctggggccat tctgggcctg ggtttcctgc
    25681 gcgattcgca ggccatcctt ttgcttggta ttctcattac cccgctcatg atcccctggg
    25741 tgggcttttt gctcgccagc cttacagggt cacctcgttt tctttttgaa acactgatgg
    25801 ccctgctcat cagcggcgtc attgtgttca ttggcgggtt gctttcgggc tttgccgctc
    25861 gactcttttt acccatcacc cttaccaacg tttttatcca tgcacgtctt tggcccgaag
    25921 aactctttgt gctcgccatc ggtgccatta cgcttgttgc atccttcgtg cgttctgaaa
    25981 gcaaaccctt cctgccaagc gtcatcgttg catatacctt ctatttgccc atcaatgcaa
    26041 gcggcttcgg cctcggctcg ggtgtggatg gcatctggcc tgaaggtatc ctcgtcttcg
    26101 ccacacactt catgctcgca agcattctcg gcctgatcac tctgctggta ttacggctgc
    26161 gcccatcaaa ccgcggcctg atcctcagcg gcgtcatgct gcttgttttc accggcaccc
    26221 tcttcagttt gatggggaac ggtttcccgc tcacaacacc ccaaaccgtt agcacctcca
    26281 cccccacacc ttcgacgcaa cccacagcga ttccttccct aacccctagc ctgccgccta
    26341 caacaacaaa cacggtccgc ccctcgaaca cgcccaaacc taccaacacc ccaagtgtgg
    26401 atgagaccct ttcggccacc ccaacagaag aaaccgaaaa tacagccacc aacacctcca
    26461 ccgctacaaa acccgccgcc acccccacaa aaacgacctc cactccggcg gccacctcaa
    26521 ccaacgaacc gatcccaacg gtcacaatga ccattgaagt gcaaaccatc cccggcacga
    26581 tcagcgcgga tgaaggcggc ggcgccaacc tgcgccaaac ccccaacggc aaatacctga
    26641 tgaccctcga caataacacc gtcgttgaaa tctaccccga tttcaaagtg gttaacggca
    26701 tcacctggct gcacgtttac gtcatccgca acgaccagca gatcgaaggc tggcttttgg
    26761 aatcagttgt cagttacgcc actcccgaac cgaacttcga accatcttcc acacctgaaa
    26821 tcggaattac acctgcaccg taaaaatagg atgaaatatg aaggatgagg gatatatcaa
    26881 tccttccgcc ttcatatttc atcctttatc tttcatcctt catccttttg gagaccccca
    26941 catgcccatt cactttggaa ccgacggctg gcgcgccgtc atctcagata gtttcacctt
    27001 cgataatctt cgcatcgtta cacaagccat tgcagatgcc gtggcatccg accattggga
    27061 caaatctgtc aatggtgaga acggacccga taaaaagacc atcgtggtcg gctacgatac
    27121 gcgttttctt tccgaccgtt ttgctggaga ggtggcgcgt gtgctggccg ccaacggctt
    27181 cactgtgtta ctggcgcaat ctgattcacc caccccggcg atctcatttg cggtgaaaca
    27241 tcaacgcgcc attgcaggca tcatgatcac cgcctcacac aatgctccgc gctacaacgg
    27301 attaaaactc aagggagcat tcggcggctc agcgctcacc gagcaatgcc gccgcgtgga
    27361 agtgtacatc aacgacaacg aagaacaggc acgcggcccc aacctgatgg attttaataa
    27421 ggcgcgcgat gcgggcttga tccaaaaatt caacccgttg ccagcttatt tcgaacattt
    27481 acaaaaattg atccgcaccg acatcattgc ggataatccc caacgctttg tggtggatgc
    27541 aatgtatggc tccgggcgcg gcgtcatcaa atcctttttg cagggaacgg gctgtgagat
    27601 cgccgaaatt cgcggtgaaa tgaaccccgg cttcggcggc gtacaccctg aaccgatcgc
    27661 aaaatacctc ggccctctgg caagcgcggt cagttcaggg atgggcaatt ttggcgtggt
    27721 caccgatggc gatgccgacc gcatcggcgc gatggatgaa cgcggcaact ttgttgaccc
    27781 gcacaagatc atggcgctct ctttgaaata ccttgttgaa aagcgcggct ggtcgggagc
    27841 agtggtccgc acagtatcca ccactcgcat gatcgaccgt cttgcaaaac gctacggact
    27901 gaaattgtat gaaacccctg taggcttcaa tcacattgcc gactacatga tgaaggaaga
    27961 tgtactcatt ggcggcgagg aatcgggcgg catctctttc aaggggcaca ttcccgaagg
    28021 tgatggtccg atcatgggtt tgctgctggt ggagatgatc gccgaagcaa agaaatcgct
    28081 cgtggaaatg gtggatgacc tgctgacaga tgtaggcccc gccttgtacg agcgtgtgga
    28141 tttgcgcctc agccgccccg tagccaaagc tgaaatgacc gaattcctca cccaaaaagc
    28201 gcccaaggag atcggcggcc aaaaagtgga tgaaatcagc cagcgggacg gcgtgaaata
    28261 catcatggcc gatgactcat ggctgttgat tcgcccttcg ggcacggagc ctgtattgcg
    28321 tgtgtacgca gaaggtcgaa acgcggatat ggtcaaagcg ttgatgggat atggaaagac
    28381 agttgctgaa agtgtagtgt aaacaaattt agtgtgaaac agaaatggac actgaccttc
    28441 gctgcaaaga gcgaaggtca gtgtgttttt aggtaataaa ggccacgatc aaacaccaaa
    28501 gaaattcaag ttgcaaccag ttcatgaatc ggcggcggga agtgtaaagt aaaacgtggc
    28561 tccctgcccc gggctgctct ccacccagac cttgccgcca tgcacttcaa cgatcctgcg
    28621 tacaagagcc aggccaaccc cggtgccctc ggatttcgca tccaattttt caaagaggcc
    28681 aaagatgcgc tcgtgatgta tcggctctat gccaatgcca ttatcacgga caaaaaaggt
    28741 cagcagattc tcctgcctgt cgcggaagcc gatctcgatc tgcgggttgg gctggctgcc
    28801 catgtatttg gaggcattgt caatgaggtt ctgcatgacc tccacaaggc gcggtttatc
    28861 tccatagaca gctggcaggt tcgggtgagt ctgaagcgtc gctccacttt cctgaatctg
    28921 tccgtgggcg atatcaacgg cttcgcggat caattcatca aacggaataa tttgagatgc
    28981 gttgaccatg cgtccgatgc gggagagttc gagcaattcg ttgagcaggg tctgcatttt
    29041 tcggaccgct tcctgaatcc gctgcaaatc gattttgagg cgatccattt tcccagcgag
    29101 ggcatccttt tcaagataac caagataccc gctgatggtg accagtggag atttcaggtc
    29161 gtgcgagacc gtgtaggtaa aacgttcgag ctcgctgttt ttattttcca attcattgac
    29221 cagtttctgg cgctcattta actggttctg cacatctgaa aagaggcgtg cattctcaag
    29281 cgcaatcgag acctgattgg cataggtcag cgccatctgt gcatctgcct cagtgaactg
    29341 gtttgccgta aggctatcca acgcaatgaa gccaagcagc ctgccgcgca gtttcaaagg
    29401 aacagaaagc cagccatgaa gaaaatctgg gaactgggga taatcctttt gaatatcacc
    29461 gatcaaaata tacggaagtt cctgcgtcca aaaaatataa tctggctcgg tttcattgac
    29521 gatataactt ttatcctgct cggtcagttc gggcaggcca atgccgccca ttaattgagc
    29581 gacccgccct ttatacaacc agaccgaggc gctgtcgtaa ctgaccacac gttttagctg
    29641 ttcgagaatt cgctgcacag tttctgaaat atcgagcgtg gaaatcacaa tggtcgtcgt
    29701 ttcgcggatg gtttcggctt cggcattttt caggcgcagg tctgcaatga gtcgttcgcg
    29761 ttcctgctcg gcccgtttgc ggtcagtgat atcaagtgcc gaggtcaggg tgtacatgcc
    29821 cgcatccaca taaaaacgtt cgctggaaag cagggcaaca cctgtctctc cgttcttacg
    29881 gcggaaaccg atctccaggt ccttgatggc acccttttcc tgaaaagtat cgcgcaattt
    29941 atgcacatcc tgcgggctct ggaacacccc cagctcggag gcgctttgcc cgatgacatg
    30001 ctcgcgcttc aggccgagcg tatccatgaa ggatttgttc gcatcaataa aacggccttc
    30061 actattttct atggtcatca ataccggggt ggtgtgaaaa gccttggaaa atttctcttc
    30121 cgaaaaacgg agtgcaaatt cagcgcgcct gcgttccgct agttcatgtt ttagtgcgtc
    30181 atcggtcaac tggcgctcaa tggcagcacc cagcacactt gccgccactt taatgacatc
    30241 cacttccatg gtggtccatt cacgttcatg atagatatcg tccacgccca atgtgcccca
    30301 atgcgttcca tttactatga tgcgaatctc aagcagggct gccacattca tggagtgtaa
    30361 ataggccttt tcttcggttg taaaaaaaga tgcactccca accaaaggtt caccgctatc
    30421 gaggatctta taaaggcggt caaaccccat tgggtttttg ggcatatttt gaaactcggg
    30481 ggtatcgagg tcggtttcca ggccgggggc ggtccattca tggatcatgg atgagaccac
    30541 ctgatcgttt ggagcagcat gtttctggaa caaataggca tgagatgaat taaattccct
    30601 gcccagcctc tcaaggacca catcaatggt ctcacgccaa ttggcggttt ttaaaaactg
    30661 ctcagcggca aaagtaacag cctcaagcaa agactcgtgt tttttcaaat taaattcagc
    30721 gagtttgcgc tcggtcacat cttgaaggat gccaatggat gcataggaca cttctgaatc
    30781 atcgaacatc tcaatggcac tgtcattcac ccagcgctcc tcgccattcg gcagagtcac
    30841 acgaacatcc gactgccagg cacggatctt ccccgaccgc acctgctgaa ccacatcttc
    30901 aatgcgctgt ccacgccctt ccccaagggg aatggcctcc atcacgatct tattccaggt
    30961 atcgggagaa aaatcctgcg gagaatatcc gacgatccgc tgaattccat tgccgatgaa
    31021 ggtataacga ttctgccgat aatcctgata atacggaacc gccccagccg cctcaatggc
    31081 ctgacgatag atccgttctg actcctgtaa agctgaatca gctttctgac gctggatcgc
    31141 cgcactgaga attccacccg cgatcttgag cgcatccaac tcagaaaaat tccaggtcct
    31201 ctccacttcg taatcatcga aaccgatcgt tccccaccat tgtccattta caaaaagcgg
    31261 aacctccaaa atggatttaa tacccaacga ggaaaagtag tcccgttcgg caggctgaaa
    31321 agtggaggta ttcccaacaa agggttggcc gttcatcaaa atctggtgat aatcttcgaa
    31381 gccttcatca ttcaggggag agttctgaaa ctccagattg ttcagatcgc tggcatatcc
    31441 gggcgcagtc cactcatagc gcatggagtc gaccatttca ttatcgggtc cgttgtgatg
    31501 ttcgaacagg taggcatgag aagcatggat ggtccgtccc agcctttcca gcacgaggtt
    31561 gatgctgtca cgccaatcag atgatttcaa aaattgttca gctgaaaatg tgacagcttc
    31621 aaaaatggcg tcgcgttcgc gcaagtcata ttcgacggat ttccgttccg caatttcctg
    31681 ttgcagcagg gaaaagccat cgaagaggaa ttcggtcagt ttataggtga ggatcgccgt
    31741 cgtcagaaca aggatcgagg cagaaaagaa atctcctgcc acgctatgca ccaacacccc
    31801 gggtgtgtaa agcccccaca attcaccgaa aaccagccag ccgatgcaca aaacggtcaa
    31861 gatcaccaag gagaacagca tccactttct ggtgaccagg cttcccatga caagaatggt
    31921 ggaaaaagca ataatgttga tgctgtggat tcccagcccc caagtggcaa gcagtgtgtt
    31981 tgaaagaatc aacatcacgg caagcacacc ggcagccaca tcgaacttct cacgatatac
    32041 cagatatgtg gaaatgggca taaggaacag gttgccgcca atgatgaggg cggcggttac
    32101 atcgttggtg gatagcacaa caagaatccc ccccaccaaa acaagagaag ttatgccgat
    32161 aaaaaaataa aagattctgc gtaaacgact ggtatctcga aatattcctg cgattttccg
    32221 tgctgggatt aagttgtcat ccttcatggc gttcatcctg aataaaattt atgggacatc
    32281 gttctgaaag ggtgtggttt gaacctgtca ggaagggctg gcaggcaatg taaaaaagaa
    32341 tgtggctccc ttacctgcct gtgattccac ccagatgcgc cctccatgaa cctcgataat
    32401 gcgtttgacg agggccagcc ccacacctgt cccttccgat tttgcatcaa gcttgttgaa
    32461 caggccgaaa atacgttcgt ggtgttcagg gtctatgccc atgccgttat cttttacaaa
    32521 gaataccaat tggccatttt caacgccttg atgaccgatc tcaatgcggg gtgagggttg
    32581 gttgcccatg tatttcgccg cattatcgat caggttttgc aggacttcgg taaggcgctg
    32641 acgatctcca tatacggctg gcaggcctgg gtgagtcccg accgggggga tgatctcgat
    32701 ggtcacattt tgtgcttcaa gcctcccatg gacgatctct ctggcatcat tcaccaatgc
    32761 ttcaatatta atcgtttcag gcggattcat gatgcggcca atgcgtgaaa gttccaacag
    32821 atctctaagc aacaaatgca ttttatccac tgcatcctga atgcgttgaa tatctgatcg
    32881 gacacggtct aaattccccg aagcggtatc acctttcagg tagccgagga atccgttaat
    32941 ggtgacaagc ggcgatttca ggtcatgtga taccgtgtag gtgaatcttt ccaactccgc
    33001 gtttttcaat tccagctctt tgatgagctt tccacgttcg cccaattcgt tctgcaattc
    33061 agaaaagagg ctggcatttt ccagcgcaat ggcaacctga tttgcaaagg tgacggcgag
    33121 ctcggtatgg tgttcattga attgatcttt tgacctgcca tccaaagcga tcaggcccat
    33181 gatctttccg cgggttttca atggaatggc cagccatgag ttgacatagg tatgtggttc
    33241 gtgcctgaag tcctttaatt cctcctgaac attgttattc aacacatagg gaaccttgcc
    33301 cagaagaatg gggagcgccg aattatcttc attgatatcc aattcgaatt cattttgttg
    33361 gaatgtgaca ggcaggttac ggccacagat gaacttctga cggttatcct cccatttcca
    33421 cacggaggca ctatcgtaag ggatgacgcg cctgatctgg tcgaggatgt tttcgacgat
    33481 ctccaaaaat tcgaacatac ccacgatgcc tgccaggctt tcacgcaggg tttcagattc
    33541 tgcgtttttc gccgccagtt tctgaataag ggtctcgcgt tcggcttcca cttttttgcg
    33601 gtcagtgatg tcacgctgaa tgccaaagaa accggtgatc cattttcgtt cgtcatacat
    33661 gcagacgtag tccccttcaa accagaccgg cgtgccatcg gtcttgcgtt cataggtttc
    33721 aaggtgcagg tgaccttcgt cgaatagttt cctgcgcagg ttcaaccctt gctgcaagtc
    33781 gtgggtaaaa atatctctgg aggtgagcga gagaaaatgg tggcgggtga tctggtattg
    33841 ttccaacatg gcgttattca catcagtata gcgctgggta ttgaagatat agagaagtat
    33901 ttcttcttta tcagcggcat ccgcccaggg caggggtttc tccagtatgc aaaagaagaa
    33961 gccatcaagt gactggttaa aaaatgcctg caaccgttgc tggttttgcc gcagggtcat
    34021 ttcggccagt tttttttctg taatatcttc aactgtccct tcaaaataaa gcggaccgcc
    34081 gttcttatcc cggatcaact ttgcattctc gcgcacagaa aagaaggtgc catcagatct
    34141 catccaaagc gtttcaaaat cacgcatctc cccatccctt tggatcgcct gcaaaaattc
    34201 cttacgccca taatcggtaa tgatgggttc acctgcctga tcttgttcga gcaattccgc
    34261 cacactctcg tatccaagca tgcgggccag catcgtattg gcaaggagaa tattcccctt
    34321 cagtgaaata cgataaaggc cgatggtggc gccttcaaa
//
LOCUS       DAOQLC010000034        34264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_390_length_34264_cov_27.074980, whole genome shotgun sequence.
ACCESSION   DAOQLC010000034 DAOQLC010000000
VERSION     DAOQLC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_390_length_34264_cov_27.074980"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..512
                     /locus_tag="PL000_08025"
     CDS             114..512
                     /locus_tag="PL000_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HNC88885.1"
                     /translation="MTEKKIRVLVAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQT
                     PEMIAEAALQEDVDVVGLSILSGAHMALTPRLLELLKMNGQSHVKIFVGGIIPDEDLH
                     RLKEMGVTGVYGPGASTEDIIRDVKLAVAG"
     gene            656..1684
                     /gene="meaB"
                     /locus_tag="PL000_08030"
     CDS             656..1684
                     /gene="meaB"
                     /locus_tag="PL000_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNC88886.1"
                     /translation="MTYSQAVLNGERLALSRLLTQVENDAPESRAALIELFPHTGKAH
                     LIGVTGSPGTGKSSLVNQLALHYRKTAQKRVAVIAVDPSSPFTGGAVLGDRVRMRDLS
                     GDPGVFIRSMATRGSLGGLAQSTANMAQVFDAAGYEVIIIETVGAGQSEVDVARLAHT
                     TLVVEAPGLGDDIQAIKAGILEIADILVVNKADRPGVENTEKALKSMLDLAHPTERIF
                     QHHGTAMRISSGPTSGGPDSKQEASSAPIWIPPIQRTVSTEGKGIVELTDAIARHVAH
                     LTQSGDWAVRERARLEVELDALIQETLVSRFREKVSQGQYENALERIFQRKISPWEAV
                     KSLMDGDA"
     gene            1684..2238
                     /locus_tag="PL000_08035"
     CDS             1684..2238
                     /locus_tag="PL000_08035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC88887.1"
                     /translation="MSIIYGKRIRLRAAEREDVATFHKWVNDPEVTRHLVLSLPMSMV
                     DEENWFNSMNQRPQNEKVLVIEIREGEGWRMIGNCGVFAVDSVSRCGELGIMIGEKSE
                     WNKGYGTETIALLAQHCFETLNFNRVFLQVYAENERGKHAYEKAGFVEEGRLREAVYK
                     HGKYDDVFVMSILRSEWNAKKGKE"
     gene            2239..3228
                     /locus_tag="PL000_08040"
     CDS             2239..3228
                     /locus_tag="PL000_08040"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HNC88888.1"
                     /translation="MHHVHRDLKVYGGIKLFAGTGSPDLAQKIADYLDQPVSSREVIE
                     FPNENLFVKLNGSVRGQDVYVIQTTSSPVHRNLMELLIMIQTLRLDSAARITAVIPYL
                     CYGRSDKKDQPRVPITARLVADMLEVAGADRYMTLDPHAGQVQGFFSIPGDVLTASHL
                     LVDHINNNMRSQMKDPVVVSVDLGFAKKGRNYAADMDMPIAFIEKRRHGNDAKAEALT
                     LIGDVKDRDVIIVDDEVDTGGSISQAVNVVKQNGARDVYLAFVHAILSRNGAERLASL
                     PIKHIITTDSAPLTPEKWKFLESRTTVLSVAQLLGEVIRRAHEGRSVGEMFNE"
     gene            3265..4074
                     /gene="mutM"
                     /locus_tag="PL000_08045"
     CDS             3265..4074
                     /gene="mutM"
                     /locus_tag="PL000_08045"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNC88889.1"
                     /translation="MPELPEVETFARSLKPELVGNTILSADLRWARTLATPSPSQFKQ
                     RIIGQRIVDVSRRAKYLIVHLKDYNLLIHLRMSGDLIVRTGKIKPEKHDRLLLHLSNH
                     TALAFNDTRKFGRVWLTDQPEKILGKLGPEPLGNDFTAQWLFENLHTRKRQLKPLLLD
                     QSFIAGLGNIYTDECLHIAKLHPQALSNTVSQEKAQALHKAIRAVLKEGIRRNGASID
                     WVYRGGDFQNHFRVYDQDGKPCPVCGTTIQKLTVGQRGTHVCPKCQAIERK"
     gene            4078..4815
                     /locus_tag="PL000_08050"
     CDS             4078..4815
                     /locus_tag="PL000_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88890.1"
                     /translation="MELQFATVTWPVPAVWGVGLVVLLIGFFIGFLDSNIRTNKKITA
                     AEVQAEAKIREAENKMILASQEESVSQKKDDPGLLRLKKENAHYTLEMDGRVVNKPPS
                     SEEKKRLIELITIFRPWLDNSPAPASERPAAPVQPAPTPAPVQATSPRPMPPIVTVAA
                     QAELDKSIRAMSIVNQIDTVLQARLVGTPLEKAGIRLQESPEGGVEVIVGIETFPTVD
                     DVRDAVIKREIRAAIAEWENKYVPGAR"
     gene            complement(4823..5668)
                     /locus_tag="PL000_08055"
     CDS             complement(4823..5668)
                     /locus_tag="PL000_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC88891.1"
                     /translation="MTQNVLIATNGFKGTWPSIEYGTWLASTLNTNVTLLGVNEKPNP
                     AAIDDHNPLEDVFEAAVELFQKNGVKYELLVENGNAEDVIPQWAKQRDSIVVLGPLGR
                     PQIRRLLTGRSIRHLMEEIEQPILYVPEATLPLKKILICIGGLGYEVTAEHIAMQMAM
                     KSRAEVTLLHIVPPMDLDYPTARIVRENWQNLTETDTPVGRSLREALQVAKDEGLTAS
                     VKARQGNVVEEILAEVKEGGYDLLCMGSPYSTNALRQLYAPNVTAEIAEAVTCPVLSA
                     RYKRE"
     gene            complement(5680..6174)
                     /locus_tag="PL000_08060"
     CDS             complement(5680..6174)
                     /locus_tag="PL000_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNC88892.1"
                     /translation="MFLKVNLLSPSGPASVGLLYKEAVGKIMTEEQFVEAVGFSNVLP
                     GSEALKLAMFVGFAAGGVPGVLTALLGAVLPPTVMMLAVAFVLQKFQQEDWLQGFVKG
                     MSPAIAALIIVVAWELFHVGQTKKIDRRTLFIAAVSAVALWFDLPSPLVLLGAGVLGV
                     FLFR"
     gene            complement(6213..6731)
                     /locus_tag="PL000_08065"
     CDS             complement(6213..6731)
                     /locus_tag="PL000_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNC88893.1"
                     /translation="MNILTELFWTFIKVNLLSTSGPASVGLLYHEAVGRFVTESQFVQ
                     AVGFSSVLPGSDALQLAMYIGYAAGGIPGGLVALLASILPPTLIILGVTMILHRLRRE
                     AWVGHFVEGLAPAISVLMVFTAWKIFEKGGETGPLGWAIGILSLIAMWLKAPERIVVI
                     LAGIAGIILLSP"
     gene            complement(6788..7312)
                     /locus_tag="PL000_08070"
     CDS             complement(6788..7312)
                     /locus_tag="PL000_08070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC88894.1"
                     /translation="MTNLPLKIYLGELNHTPLGDLRIAASDLGLVAVEWAESQPRLFE
                     YLFRLKGTVEHSQKATHRYTKELGEYLKGKRHDFTFEINWGTLKPFQRKALKAVYQIP
                     YGETLTYADIAMQIGHPKASRAVGRANATNPMPLVIPCHRVIGKDGKLHGYGGGDGIP
                     TKEWLLKLEGAVIA"
     gene            7553..8539
                     /locus_tag="PL000_08075"
     CDS             7553..8539
                     /locus_tag="PL000_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HNC88895.1"
                     /translation="MPTQITIIGLGQIGTSMGMALAAHKASIVRVGHDKKAELERDAQ
                     QKDAIDKAEHNLPTAVRDARIVVLTVPVSQVRDTLEFIAPDLKEGTVVLDTSPIKANV
                     AKWAKEILPADRHYVGLVPAINPVYIHDFQFGQAAAKPDLFANGLFLIDAPYGTPESA
                     VTTAVDFVRLLEALPMLTDPAESDGLMSTTHLLPQIVAASLLNATVDQPGWQEARKVA
                     GRPFATVTAGLAYQDEIDSLRLSSLQNRAGMIHALDVMIAALRGFREDIENEDDEGVA
                     ARLEAALEGRQRWLSERVAADWSGATRSEAADMPTFSERFFGGMFVKKHNRK"
     gene            8536..8793
                     /locus_tag="PL000_08080"
     CDS             8536..8793
                     /locus_tag="PL000_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNC88896.1"
                     /translation="MTCYCYIVECSDGTYYTGWTTDPERRVSQHNKGIGAKYTSTRRP
                     VKLVYLEIQPDRTAAMKRELAIKRLKREQKARLIAGDLKPK"
     gene            complement(8854..9405)
                     /locus_tag="PL000_08085"
     CDS             complement(8854..9405)
                     /locus_tag="PL000_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNC88897.1"
                     /translation="MTKHQVHPIEIAGIKRELPLFEVKPGLRIAILNILGDTELVQAC
                     ARELGKKLQAVDYDILVTAEAKSIPLAYALSVETKKPYVILRKTYKPYMGAALEAETL
                     SITTGQPQVLILDEKDRSIIAGKKVVILDDVISTGSTLQGMRMILDKTDAKVVAEAAI
                     LTEGDRAQWMHIISLGHLPLFTG"
     gene            9477..9743
                     /locus_tag="PL000_08090"
     CDS             9477..9743
                     /locus_tag="PL000_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88898.1"
                     /translation="MAIENLCYTLAMPIIRASDIGNYLYCRRSWWYRKQGFESANKAE
                     LAAGTELHSRHGRQALASSLSRTVGLILLLGALILLVAYCTAQL"
     gene            9765..10397
                     /locus_tag="PL000_08095"
     CDS             9765..10397
                     /locus_tag="PL000_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88899.1"
                     /translation="MSAVLSRLAHLQTRRDRTPNLDLARDLAARNDKAGIREIAQNMW
                     SANKNVHWDCIHVMYEIGAIDPALIAPYAEDFVKLLKSKHNNMVWGAMTALAEVAKVK
                     PEVIFKHLDEIKKAKENGSVITVDNSISVLAQTAAANEEYNKAIFPYLLKHLSACRPG
                     EVPQHAERTLPAVNESNRDDFIKTLNRRNDDLSGAAFTRLGRIIRIAENI"
     gene            10416..10928
                     /gene="cas4"
                     /locus_tag="PL000_08100"
     CDS             10416..10928
                     /gene="cas4"
                     /locus_tag="PL000_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HNC88900.1"
                     /translation="MLYLALALFLFALFFFRKSSVQRKEAGLPGGRIIYTDTRGWGKV
                     EKPLFYPALELTGKPDYLVEKNGQIIPVEVKSGRAPQAPYDSHIYQLAAYCLLVEKTY
                     GKRPPYGIIHYENRDFAVDYTHELETALIDLLADMKLDEHKNDVPRSHEQAARCAKCG
                     FRNMCDQSLA"
     gene            10971..11834
                     /locus_tag="PL000_08105"
     CDS             10971..11834
                     /locus_tag="PL000_08105"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNC88901.1"
                     /translation="MPDPFLPKNPVVTAYPKMPEAFTEAEAMVSFLKERGIQAPFGSL
                     YDEELRRRVKNGEFDMLIAVGGDGSVLRAGHLCAPCGVPILGVNLGRLGFLIQIDRKE
                     WREYFEKLFEGEAWIENRMMLRAEHIRAGESINRSNALNEVVVARGQNLRPVRLTASV
                     DNRRLTSYVADGLIASTATGSTAYALAAGGPILPPELRNILLVPIAPHLSVDRAVVLS
                     EGSSVSIVVKSENSVLSVDGQPPTPLMEDDQVKVTAADVTAQFVRFGDPGYFYRNLTA
                     HMNENSLGFPR"
     gene            11831..12406
                     /locus_tag="PL000_08110"
     CDS             11831..12406
                     /locus_tag="PL000_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88902.1"
                     /translation="MTDTEITPTATYCYAHPGRETSLRCKRCEKPICASCAQRTPTGY
                     MCKDCVNQHQKIFDTAVWYDYLTVFFTSAILSGLAAVATIIITSIIWGFFIIGLAPLA
                     GTMIANVTRRFVKNHRSPMLNTTLVVGMILGALPVLLLSGLPGLLTLLGGGGNVMGSV
                     VSFSPVLWQIVYLVIAAPVAYSQFSGLVFRR"
     gene            12410..14146
                     /gene="recN"
                     /locus_tag="PL000_08115"
     CDS             12410..14146
                     /gene="recN"
                     /locus_tag="PL000_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNC88903.1"
                     /translation="MLTELHIQNFAIIDKLDLRFDKGLIILTGETGAGKSIILDAVVM
                     LIGGKADATFVRSDSDAAFVEAVFQLSGPEKEAVTEVLTREELLDDPNYVVLMREVRK
                     EGRSVARVNGRTVNVGLLKELGALLIDIHGQAEHLSLLDPRAHLGLLDRYAEVVKPLG
                     DYRQSYQALLGLRRELVELRKAQSDSERRTEMLTYQAEEIEDARLKAGEDEELRKERD
                     RLANAESLAQNAQEALAILDEGSPDMPAATDLIGQAAQALAALAKIDPEQVELSNQAE
                     LMLDTISDVVHGLQNYLEEIEFNPKRLDEVEERLNLIHSLTRKYGGSISSVLAYGADA
                     RKQLETLTNAADRINELEMQEAKLLDKLAKLGGVLSEKRKSAALTMSKGIEAELGDLR
                     MEAARFSVDFQTKPDVNGIPLADGTRIAFDQNGFDRVEFLIAPNPGEGLKPLAKIASG
                     GETSRLMLGLKNVLARADQVPSLIFDEIDQGIGGRVGMVVGQKLWNLSRSHQVFCVTH
                     LPQLAVFGDQHYQVQKLVDQGRTLTRVERLEGEPRLLELSQMLGEVGEGTLRSAHELL
                     QTARQMVKTPKK"
     gene            complement(14344..14415)
                     /locus_tag="PL000_08120"
     tRNA            complement(14344..14415)
                     /locus_tag="PL000_08120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14380..14382),aa:Arg,seq:cct)
     gene            14453..15901
                     /locus_tag="PL000_08125"
     CDS             14453..15901
                     /locus_tag="PL000_08125"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNC88904.1"
                     /translation="MRHIILLLVLLLTACSSSTAQPIMATLPVETSTPIPPATESPTA
                     TNTPVPIPEREKYIINTFLDYDQHFIDVDQDIIYPNHTGQQLTTLTLAVPPNFWPNCF
                     YLMELQVDGNVVTNYSFNLHRLDIPLPTPLEPDSVSTISLRYSIFLPFMDQVNSQRAR
                     IFGYSDLQMNLVNWYPFVVPFKDGEWIIREPWSHGEYLVYPMADFEVNLKFANPDTAP
                     IIAASGPAEPNGEFTRYTLTGGRAFAISASRDFLVSSTQVGETVVYSYHLPIYQVAGE
                     AAMLTSAQAIQVYSQMFGPYPHKTLSVVVADFQDSMEFSALYFHSRSFYNLYDGTPQS
                     YLVSVAAHETGHQWWFDQVANDQAREPWLDESLTTYSEAIYYEAAHPESLAWWWTNRI
                     DFFQPFGDIDIPVYEGQDADTYKYIVYFNGAHFMRDLRGRIGDAAFFAFLQDYYNQGR
                     GKIVTGDDFFRILDQHTDMDYSDLVSTYFKNR"
     gene            15914..16342
                     /locus_tag="PL000_08130"
     CDS             15914..16342
                     /locus_tag="PL000_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88905.1"
                     /translation="MKSDVIKPMLIIMAAPIFSALLSVFKIVPAPPYQGFASLLYLPI
                     CALSLPVAILLGLQKKLLGVNLLVGLIVSFIFPFALFILINPLWMPSGMSNCKEGTPP
                     SATVAHYECEDSSSDDGSYHRDFIIEGYKGWPLMRVIEDK"
     gene            complement(16427..17380)
                     /locus_tag="PL000_08135"
     CDS             complement(16427..17380)
                     /locus_tag="PL000_08135"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88906.1"
                     /translation="MQRVSFFITLLVISALACGASQSQPSAAVGPVETAPSPQVKEET
                     GVSSSAFDSQKLGVVERDVTYCIMNGIELKMDVYYPSSNDGPWAAAMYIHGGGWSNGD
                     KADGAGAKEIGPLRAAGFLVVAVNYRLAPQNLFPAMIEDVKCAVRSLRAHADEYNLDP
                     DRIGVWGGSAGGHLAAMLGTTDKNAGFDVGEYLDQSSRVQAVVDMFGPADLTVPFEGG
                     YESARRVFDGFDAARASPVTYVSADDPPFLILHGTEDELVPLEQSQILLARLQAAGVP
                     AELVIVANAAHGFTPAPGGISPSRREISQRVVDFFVKYLGG"
     gene            complement(17488..>18442)
                     /locus_tag="PL000_08140"
     CDS             complement(17488..>18442)
                     /locus_tag="PL000_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88907.1"
                     /translation="ISFIINVHDWNHPEESAELLLKLVDLFEQYNIRGDFYFTAEITR
                     QLAEKRPDVIERFKNSNMTISYHVRPPHPLYNGFDAGLQSMSDAELYQTLLDYETYAL
                     NLETGELDRSRPGGYTYVAQVFGRNPVVAPVPNSGRIKDAGQKVYASLGAQMTLLYHE
                     SGTDLEQPFEYVNGLLVRPSDFSITRTTVIDGADNFWWNFMSKPNADLYDPLKMLQTQ
                     LSEWDAHGYNREPFITSLIHENNFYRSGAEAWTSIYFSMQNNKKVQPLSPPYDLNAPD
                     PSMLRTEEDQQAIFAAYEKMVAYAAAHLHVVTSEDILEMAE"
     assembly_gap    18443..18542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18543..18743)
                     /locus_tag="PL000_08145"
     CDS             complement(<18543..18743)
                     /locus_tag="PL000_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88908.1"
                     /translation="MRLIRTLILITLFAVSCTPTSPPPPPTTATVTESSPTFSIQSPI
                     TSPASISFIINVHDWNHPEESAE"
     gene            complement(18831..20903)
                     /locus_tag="PL000_08150"
     CDS             complement(18831..20903)
                     /locus_tag="PL000_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC88909.1"
                     /translation="MRRFIQITLTLLFALALTLGFFSAAPVSPVRAAGVTYYVAVNGS
                     DANPGTLDQPWQTIQHAADSVSAGDTVQVRGGTYNETVSINVSGSDAGGYITFQNYSG
                     ETAILDGSGFSSAAGDIAFTIENQSHISVNGFEIRNYKTNQAGATPMGIFVTGASHHI
                     QILNNYVHHIETNAGANGNAHGIAVYGTAAPASIHDLVIQGNQIRDLKLGSSEALVLN
                     GNVENFIVTQNTVTFCDNIGIDLIGFEGVSPNPSYDQARDGIVSDNTVTAIDTITNPA
                     YGGSQSAAGIYVDGGTRILIERNKIAASNIGIELASEHQGHATSYITVQNNLISYNHI
                     AGLAMGGYDTQRGSTENSTIINNTFFENDQNQDGNGEIWLQYNVNNNTIQNNIVFANS
                     QSWLITNPYTQNQNNVVDYNLYFAPAGGDGSEWQWKNVFYQGFAAYQAGTGNDAHSLF
                     TDPQLTGTTVFDYHLLSTSPAIDIANCANAPTDDFAGDLRPQGAGCDIGADEYTSQLF
                     ADVPADYWARTWIETLYASGITGGCGTNPLTFCPEAPVTRAQMSIFLERGMNGAAYNP
                     PTASGTVFSDVPADYWAAAWIEQLASDGITAGCGNGNYCPEDYVTRDQMAVFLLRAKY
                     GSAYTPPAASGVFSDVPADYWAAAWIEQLANEGITAGCGNGNYCPTQLVSRAEMAVFL
                     VRTFNLTP"
     gene            complement(20961..23054)
                     /locus_tag="PL000_08155"
     CDS             complement(20961..23054)
                     /locus_tag="PL000_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88910.1"
                     /translation="MKKFQNLILLLAFIMTACMPGKPPKQAPQPAPETDSVSTGIPAP
                     QIVPTVQADCVEPICLTASISLLDLQNRSVDENVLKLFSAAVDDENNRVYVAGIMTLD
                     VAVLDGATETWIGTVETGLTERGIKYIYFDSKAQYLYIYDTVRKELRRVDVESGEIVG
                     PVPLAEGGFGQLATVDETHTRFYLTTSKGLVAFDGNTLEQLYTVSGLGESAGPMVYDP
                     AKDVFYLLDTTSKEADRNIFILAAASGEVTGSIHYTAMQGIRSRWLHFDPADQHFIIG
                     MDRSVAIFDMNGTEISAFPLSGEHIVEDQVYDPATKQVFVSSINSPQNGQVAGIGGHM
                     DVYDLSGNRTSSFDFGRKPHHMNLNPANGHIYIPNGDASVVWSVDTHAPTQATSLRLG
                     DSVEQIVSSGDGSTLYFASRLGGSYIGSYNQDSGAFEAFTAGTWPIPIRRDASGEHLF
                     TLNAWDGTLSVYSITPNQTLLGTIPLGTPAGSTDRLPDLAIDSTHKLAYAAYPEFGTI
                     AVVNWETMQPVATIPVEGAETGDTGGGPGQLQVTVNETENRVFVYDSKSRRLWIYDAA
                     NQFSPLGSVDLREASRSIGQSGADLFFFDARTNRIYLGALELDAVTGQPSGRTLSAGD
                     KIFGMDSASNTYWVFDSASNAILAVDRETLSVRFSQPLPESASFLPTLAYNPARNQIH
                     VGYLATAHVYTFTVR"
     assembly_gap    23127..23226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23268..24308)
                     /locus_tag="PL000_08160"
     CDS             complement(23268..24308)
                     /locus_tag="PL000_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88911.1"
                     /translation="MKRISHLLILLVVLSTACNLPGSLTAQAETLPLETATQPGAESE
                     SGEAILLFTIGMHIEPMGETAQGYKSGKGDHHNPVFFDKQVQDIRTVTQIVEAHGGRM
                     TIQAQSPFTTVAIESGNSILADLAASGHEIGLHFHEDAHLGKNSETLTVQTWCDVMSE
                     EIELIEQAASVEKIRFWSGGNLNPNLYEAAECAGLSVNSDWKNRMTQETPLDLVGVNP
                     WRPAGGTDGTDFNLFTRHDPHGAIIFLPEGQYDKTNFASMRRSEDTGGDEAYFEFLKQ
                     SLYASLDAAEAGEVNVFHFTIHPGEFRGDPQHPFEVIEKFLSEVVDPLVASGQVQWAT
                     FSEMADAYAEMH"
     gene            complement(24612..25640)
                     /locus_tag="PL000_08165"
     CDS             complement(24612..25640)
                     /locus_tag="PL000_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC88912.1"
                     /translation="MRYQLLGRSGLRVSEASLGTMTFGETWGWGASKAESKKMFDLFT
                     KAGGNFIDTSFNYTDGTAEEFLGDFLKTDRDYFVLASKYTLTKPDNTDPNGGGNSRKN
                     MMRSVEQSLKRLKTDFLDIYYLHVWDYMTPVDEVVRGLDDLVRQGKVNYVAFSDSPDY
                     IVAEANTRAELMGWSRFIGMQIPYSLLDRAVERSVIPMAAHWEMAVLPWGLLEAGILT
                     GKFLKSIKNSTRLNPKKLRLSEKQLSVVQEVQRISEETGKPMSQVAINWVRQSPKAQM
                     IPILGARTASQLKDNLAAFDWKLDDDQYQRLEKVSAIEMGFPYDFIPGNPYVYGATFD
                     KIDRPIRR"
     gene            25955..26551
                     /locus_tag="PL000_08170"
     CDS             25955..26551
                     /locus_tag="PL000_08170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC88913.1"
                     /translation="METIRGRLSHFLDSTELQARLNPYNRLTLDLGTGDGKFAFCRAD
                     AFPSHFVIGVDSCRENLHEYSRAKLPNLLFIIASAQSLPHELHGLASHITINFPWGSL
                     LESLLNGDDRLLHGLQSVARSPAALDIRLNGGALAEQGWALEDGTERIFENLTSTGWK
                     IRPPEMLDSHALRAFPSTWAKRLAFGRDPRAMQLSGCL"
     gene            complement(26706..27413)
                     /locus_tag="PL000_08175"
     CDS             complement(26706..27413)
                     /locus_tag="PL000_08175"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNC88914.1"
                     /translation="MEVTIKIFRYNPEKDKRGNYVTYKVEADENDRVLDVLEFIKGKT
                     DGTLSFRRSCAHGVCGSDAMRINGRNMLACKTLIRDVGANITVEPVLGLKVKKDLIVD
                     MEPFFDNYRKMLPYFINDSPLPENGRERLQSPEQRARFDETTKCILCAACTTSCPSFW
                     ANEEYYGPAAIVTAHRFIFDSRDEAADKRLQVLAETDGIARCHTAYNCTEACPREIKI
                     TKAIGEVKMAIITGKLD"
     gene            complement(27422..29215)
                     /locus_tag="PL000_08180"
     CDS             complement(27422..29215)
                     /locus_tag="PL000_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC88915.1"
                     /translation="MANVHQFEVVVVGAGGAGLMAGLYASRGAKTAVISKLYPTRSHT
                     GAAQGGISAALGNYEEDKPEWHMYDTVKGSDYLGDQDAIEFMTNEAIDAVLELEHMGL
                     PFDRTPEGRISQRPFGGHTNNVTGKPVRRAAHAADRTGHMILQTLYQQCIKNDVTFFD
                     EYQVLDFIMVDGKAAGVVAIELATGELHTFHAKAVIFATGGHGRIFEVTSNAYAYTGD
                     GAAILLRHGIPLEDMEFFQFHPTGIYKLGILITEGVRGEGGILINGNGERFMPKYAPT
                     VKDLASRDVVSRAIYTEIKEGRGINGSNYVYLDIRPESVNKFAALDGRTNPDGTPYTI
                     TGETVMQKIPDIVDFCRVYLGVDPVTQVMPIQPTAHYTMGGIPTNKFGEVVTDDKGTA
                     LPGLFAAGECACVSVHGANRLGTNSLLDLVVFGKHAGLRAAEYAKSAEWVKMPEDAEA
                     GTRSALEALRKGSGKENAFDIANEMKKVMFADVGIYRNEKDMQSALEKVRELQGRFKN
                     IGVTDTGKIFNTELLNAWELGNMLEIAEVVAASALNRKESRGGHSREDFPDRDDANWL
                     KHTLIAKKNGKLEINYKPVVITKHQPKARVY"
     gene            complement(29296..29730)
                     /locus_tag="PL000_08185"
     CDS             complement(29296..29730)
                     /locus_tag="PL000_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88916.1"
                     /translation="MTTKTRTLSIKQRGLTNFESIMWIFTRLSALAMYALIIFGMIGA
                     LVMGAREQMNFADVMRWAFMPNVTHVQSTDVPDLNPWSSPFWKLTASALLFVAVAHGV
                     HGLVVIADDYITTEGGRKIVRLLSIIMMGSMSLMGLYLIWTS"
     gene            complement(29732..30106)
                     /locus_tag="PL000_08190"
     CDS             complement(29732..30106)
                     /locus_tag="PL000_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88917.1"
                     /translation="MGLKRNVSLSTGLRYKGQGPMLTFVLHRIGGLGMAVFITTHILA
                     SFLGGKGGAFVNSIYENWVFQIFIFFCVLFHAINGLRITILDLFPKLIDHHREAIWIE
                     WAVFIPVYAISVFVIVTTALGG"
     gene            30488..30985
                     /locus_tag="PL000_08195"
     CDS             30488..30985
                     /locus_tag="PL000_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNC88918.1"
                     /translation="MSGSNELSRRDFIKVTTGIVGGLIGVALGLPSVSYLIDPALQEG
                     GKDAWISIGKFEDMQLEKPYPFSFTRVQVNGWERTSSSFGGYAIRKSDDPKDLLILNS
                     RCTHLSCNVNWHEESDAFLCPCHDAKFSRDGNVLDGPPPRPLDVYEEFRLSEDGNIEI
                     FFKAG"
     gene            30989..31648
                     /locus_tag="PL000_08200"
     CDS             30989..31648
                     /locus_tag="PL000_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HNC88919.1"
                     /translation="MWKTVYEWLDERLGLNDLYTNLLDRPEPKGNWWNTLGSASLFLF
                     VLQGLTGIFLTVYYTPSPDHAYDSVQYIMEGVAFGWLIRGIHHWGATLMVIVVFIHLL
                     RVFITASFKFPRELTWLIGIGLFLATLGMGFTGYLLPWNQKAYWATTVGTQIAGSVPF
                     IGEFILKALRGGPDLSALTLQRFFSAHIWIIPAVLAGLIGIHLYLIIKHGESHFPTKE
                     D"
     gene            31678..33066
                     /locus_tag="PL000_08205"
     CDS             31678..33066
                     /locus_tag="PL000_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC88920.1"
                     /translation="MNEETKKKINARYEKELNKGERFWPDSIFKDLVVSLGIFILLVL
                     LASFVGVAPEPKADPSDTSYIPRPEWYFLFLFKFLALYGQIPLLGKIEWLATVLVPGI
                     AIGVLTLLPFIEKSPDRHYAKRALSISVMTIMVVGIVLLTLMSEIPTVSADGSKLLGL
                     LQAMAGLAVPGLAMIALFVAALAFKTTNTRFFIWTTSLTVVSMIILSGTVMALHTPPA
                     VEETEVATTLVDQIFAGQDLYSVHCTECHGDDGSVAVIEGVEGLEGEKITPINSHDVL
                     YTITDSAMYEVIAYGRPNAGMTPFGKAYGGELSKSEIDYIVTYMRYMWDDRFEIPAEA
                     LKPLFPPLAEGEVPSYEVHIQPIVKRYCVSCHRAGKENNNYLMTSYEEMLTTGDNAAN
                     NIIAGDENSYLLQVIQEHAITDANGQELIGVMPPNRALKPNIVDVFIRWIMNGMPQTA
                     VDAAALFTAPAP"
     gene            complement(33151..33501)
                     /locus_tag="PL000_08210"
     CDS             complement(33151..33501)
                     /locus_tag="PL000_08210"
                     /inference="COORDINATES: protein motif:HMM:NF018672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HNC88921.1"
                     /translation="MNIVVNIITGLVAVLHGYFLILEMFLWEKPAGRKSFRTTAEFAA
                     QSKTLAANQGLYNGFLSAGLIWGIFAGDAVKVFFLSCVIIAGLFGAFTVSKRIFYIQA
                     LPAIIGLVILLVAG"
     gene            complement(33605..>34264)
                     /locus_tag="PL000_08215"
     CDS             complement(33605..>34264)
                     /locus_tag="PL000_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNC88922.1"
                     /translation="KLWDTHGDGKWKSKLMLKDSIADVTLQFVLIRPRDYDVIATMNL
                     NGDYLSDALAAQVGGIGIAPGANINYVTGHAIFEATHGTAPKYADLDKVNPGSVILSG
                     EMMLRYMGWGEAADLIVKGMEGAIHAKTVTYDFARLMDKPTEVKCSEFGDAIIHWMDK
                     EIPAPKAEAAAAKAPAKKAVAKKAVAKKPVKKAAAKKPAKKAVAKKTTKKVVKKTGKK
                     K"
BASE COUNT         7801 a         9041 c         8996 g         8226 t
ORIGIN      
        1 tgtgcgctct gtggttaaat ctttggttgg caagcccacg aaattagcca ctccctaata
       61 actgcgcttg tgatacatag tataatttca ctcatcattc cctcggagga actatgaccg
      121 aaaagaaaat ccgtgtgttg gttgccaagc ccggtctgga tggacatgac cgcggcgcaa
      181 aggtggtggc gcgcgccctg cgcgatgccg gtatggaagt gatctacacc ggcctgcgcc
      241 aaaccccgga aatgatcgcc gaagccgctc ttcaagaaga tgtggatgtg gttggcctct
      301 ccatcctttc cggtgcccac atggcactca caccgcgttt gttggaactg ttgaaaatga
      361 acggccagtc tcacgtaaag atcttcgtcg gaggcatcat ccccgatgaa gacctgcatc
      421 gcttaaaaga aatgggcgta acaggcgttt atggccccgg cgcatccact gaagacatta
      481 tccgggatgt caaactggcg gtggcagggt aacctgttca cacatacaca gagcaaaggc
      541 tctgtgtatt ttttcgctga aacaaaccca agatgaacgg agatactgaa aggttttcat
      601 ccccgttcat ctttgattgt gtcgatcatc tcttcactta tttcgaaacc actccatgac
      661 ctactcacag gcagtcctca acggcgaacg actcgcgctc tcgcgcctgc tcacacaggt
      721 ggaaaatgac gcgcctgaaa gccgtgccgc attgatcgaa ttattccctc ataccggcaa
      781 ggcccacctc attggcgtaa cgggttcccc cggcacgggc aaatcttcgc tggtgaatca
      841 actggcgctg cattatcgca aaacggcgca gaagcgggtg gctgtcattg ccgtggatcc
      901 atccagcccg ttcacgggcg gcgcggtcct tggtgaccgc gtgcgcatgc gcgatctctc
      961 cggtgacccc ggcgttttca ttcgctccat ggccacccgt ggatcgctcg gcggtctcgc
     1021 gcaatccacc gcgaacatgg cacaggtctt tgatgccgct ggttatgaag tcatcatcat
     1081 tgaaaccgtc ggcgcaggtc aaagcgaagt ggatgttgcc cggctcgccc acaccacact
     1141 ggtggtcgaa gcccccggcc ttggcgatga tattcaagcc atcaaagcgg gcattctcga
     1201 aattgccgat attcttgtcg tcaacaaggc agatcgaccc ggcgtggaaa acaccgaaaa
     1261 ggctttgaaa tccatgcttg atctggccca ccccaccgag cggatttttc aacatcacgg
     1321 cacagccatg cggatttcat ccggtccaac ttcgggtggg cctgattcaa aacaagaagc
     1381 ctcatcagcg ccgatatgga ttccacccat tcaacggacg gtttccaccg aaggcaaagg
     1441 cattgttgaa ttgaccgatg ccattgcaag gcatgtggca cacttaaccc aaagcggcga
     1501 ctgggccgtt cgtgagcgtg ccaggcttga ggtcgagttg gatgcgttga ttcaggagac
     1561 actggtcagc cgttttcgtg aaaaggtctc acaaggccag tacgaaaacg cgttggagag
     1621 aatcttccag agaaaaattt ctccctggga ggcagtgaaa tctttgatgg acggagatgc
     1681 ctaatgagca tcatctacgg aaaacggatt cgcctgcgtg ctgccgaacg ggaagatgtg
     1741 gcgaccttcc ataaatgggt gaacgaccct gaagtgaccc gacatctggt gctcagcctg
     1801 cccatgtcca tggtggatga agaaaactgg ttcaattcca tgaaccagcg accgcagaat
     1861 gagaaggtcc tcgtcattga gattcgtgag ggcgaaggct ggcgcatgat cggcaattgc
     1921 ggcgtgtttg cagtggactc tgtttcccgt tgtggtgagc tcggcattat gatcggcgaa
     1981 aaaagcgagt ggaacaaggg ctatggaacg gagacgatcg cgctgctcgc gcagcattgt
     2041 tttgaaaccc tgaatttcaa ccgcgtcttt ttacaggtgt atgccgaaaa cgagcgcgga
     2101 aaacatgcct atgaaaaggc cgggtttgtg gaagaaggcc gcctgcgtga ggcggtttac
     2161 aaacatggaa aatatgacga tgtgttcgtc atgagtattt tacgttcaga atggaacgcg
     2221 aagaaaggca aagaatagat gcatcatgtt catcgtgacc tgaaagtgta tggagggatc
     2281 aagttgtttg cgggcacagg ctcgccagac cttgcccaga agatcgcaga ttacctcgac
     2341 cagcctgtca gcagccgtga ggtgatcgaa ttcccaaatg agaacctgtt tgtgaagttg
     2401 aacggcagtg tgcgtggaca ggatgtgtac gtgatccaga ccacgtcctc accggtacat
     2461 cgcaatttga tggaattgct catcatgatc caaaccctgc ggcttgattc tgcggcgcgg
     2521 atcacggcgg tgattccata cttgtgttat ggccgctccg ataaaaagga ccagccgcgt
     2581 gtgccgatca cggcacgcct ggttgccgat atgctcgaag tggccggggc cgaccgttac
     2641 atgaccctcg acccgcacgc cgggcaggta caggggttct tctccatccc cggtgatgtg
     2701 ttgaccgcat ctcacctgct ggtggatcac atcaacaata atatgcgctc ccaaatgaaa
     2761 gaccccgtgg tggtttctgt ggacctgggg tttgccaaga agggccgtaa ttacgccgct
     2821 gatatggaca tgccgatcgc tttcatagaa aagcgccgcc acggcaacga tgccaaagcc
     2881 gaagcgttga ccttgatcgg tgatgtgaaa gaccgtgatg tcatcatcgt ggatgatgaa
     2941 gtggataccg gcggttccat ctctcaagcc gttaatgtgg tgaagcaaaa tggcgcgcgc
     3001 gatgtctatc tggcatttgt tcacgccatc ctttcccgca acggcgcaga gcgtctggct
     3061 tcgctgccca tcaaacacat catcacaact gattcggcgc cgctcactcc cgaaaaatgg
     3121 aaattcctcg aaagccgcac aaccgtcctt tcagtagcgc aattgctcgg tgaggtgatt
     3181 cgccgtgcgc atgaaggccg ttcggttggt gagatgttca acgagtaaaa cggttctttt
     3241 gaaaattccc gcctgagatt tctcatgccc gaactgcccg aagtggaaac ctttgcccgt
     3301 tcgctaaaac ccgagttggt tgggaatacc atcctctcgg ctgatttacg ctgggcgcgc
     3361 acgctggcaa ccccttctcc atcacagttc aaacagcgga tcatcgggca aaggatcgtg
     3421 gatgtttcgc gccgggcaaa ataccttatt gttcacctga aagattacaa tctcctgata
     3481 catttgcgca tgagtggaga tttgatcgta agaacaggta aaattaagcc tgaaaaacac
     3541 gaccgcctgc ttttgcatct ttccaatcac accgccctcg ctttcaacga tacccgcaaa
     3601 tttgggcgcg tgtggctgac agaccagcct gaaaaaattc tcggcaaatt gggccctgaa
     3661 ccacttggaa atgattttac agcgcagtgg cttttcgaaa acctgcatac gcgtaaacga
     3721 caattaaagc ccctgcttct cgatcaatct tttattgcag ggctcggcaa tatttacacg
     3781 gatgaatgcc tgcatattgc aaaacttcat ccgcaggcct tatcgaatac cgtatcacaa
     3841 gagaaggcgc aggccctgca caaggcgatt cgcgccgtgc ttaaggaagg catccgccgc
     3901 aacggcgcca gcattgactg ggtgtatcgc ggcggtgatt ttcaaaacca ttttcgcgtg
     3961 tacgatcagg atggcaagcc ctgtcctgtt tgcggcacca caatacaaaa gttgaccgtc
     4021 ggccagcgtg gcacgcatgt ttgcccgaag tgccaggcaa tcgaaaggaa atgaatcatg
     4081 gaactccaat ttgcaaccgt tacctggccc gtaccagccg tttggggagt gggtcttgtg
     4141 gttttgctga tcggtttttt tatcgggttt cttgattcca atatccgtac gaataaaaag
     4201 atcactgccg cagaagttca agccgaagcc aagatcaggg aagcggaaaa caaaatgatc
     4261 cttgcttctc aagaggaaag cgtctcacaa aaaaaggatg accccggctt gctgcgtctc
     4321 aaaaaagaga atgcccacta cacacttgaa atggacggca gagttgtcaa caagccaccc
     4381 tcttccgaag aaaaaaagcg cctgatcgaa ctcatcacca tctttcgtcc ctggcttgat
     4441 aacagccctg ccccggcttc tgagagaccc gccgctccgg ttcaacctgc gcccacgcct
     4501 gctcctgttc aagcgacatc acctcgtccc atgcctccca ttgttacggt agctgcccag
     4561 gccgagttgg ataaatccat ccgggcgatg agcattgtca atcaaattga taccgtgctg
     4621 caagcccgtt tggtcggaac accgctggaa aaggctggaa ttcgcctgca agaatccccc
     4681 gaaggcggtg tggaagtcat tgtgggaatc gaaacattcc ccacagtgga tgatgtccgt
     4741 gatgctgtga tcaaaaggga aatccgcgcc gccattgcgg aatgggaaaa taaatacgtc
     4801 cccggggcca gatagaaaag ttctattccc tcttgtatcg cgccgataag accgggcagg
     4861 taacggcttc agcgatctca gccgtcacat taggggcgta aagctggcgc agtgcatttg
     4921 tgctgtatgg cgaccccatg catagcaggt cgtatccacc ctcttttact tctgccaata
     4981 tttcttcaac cacatttccc tgacgcgcct ttacactggc ggtcaggcct tcgtctttgg
     5041 ccacctgtaa agcctcgcgc agactccgcc caacgggcgt atcggtctcg gtcaggtttt
     5101 gccaattttc gcggacaata cgtgccgtgg ggtagtccag atccatgggc gggacgatgt
     5161 gcagcagggt gacttcggcg cggctcttca tcgccatttg catggcaata tgctccgcag
     5221 tcacttcgta gccaaggccg ccaatgcaga tcagaatctt tttcagagga agggtggctt
     5281 ccggaacata caggatcggc tgttcgattt cctccatcaa gtgacggatc gatcgccccg
     5341 tcaacagacg gcggatctgc ggacgtccca gcgggccaag tacaacgatg gaatctctct
     5401 gctttgccca ttgcggaatg acatcctcgg catttccatt ttccacaagc agttcgtatt
     5461 tcacgccgtt tttctggaac aattccacag cggcttcgaa cacatcctcc aatgggttgt
     5521 gatcgtcaat ggcggctggg tttggcttct catttacacc gagcaatgtg acgttcgtgt
     5581 taagtgtcga ggcgagccat gtcccatatt cgatggaagg ccaggtgcct ttaaatccat
     5641 tggtggcgat taatacattt tgggtcatat attttccatt caccgaaata agaatacgcc
     5701 cagaacgcct gcacctagta aaaccagcgg agagggcaga tcgaaccata aagctacggc
     5761 gctgacagcc gcaatgaata aagtgcgcct gtcaattttc ttggtttgac caacatggaa
     5821 gagttcccac gccacgacaa tgataagtgc ggcgatggcc ggactcatac cttttacaaa
     5881 tccctgaagc caatcttcct gctgaaattt ttgtagtaca aaggcaacgg ccagcatcat
     5941 cacagtgggg ggcaacaccg cgccaagcag ggctgtcaac acaccaggca caccgcctgc
     6001 tgcaaagcca acgaacatgg caagttttag agcctcactg ccgggcaata cattggaaaa
     6061 accaacagcc tcgacaaatt gttcttcggt catgatcttg ccgaccgctt ccttgtataa
     6121 aaggccaacc gaagcaggcc ctgaagggga aagaagattt actttaagga acatccataa
     6181 caggctgctc caaacggtaa tttttgattg tttcatggcg agaggagaat gattccggct
     6241 atgccggcaa ggatgacgac aatacgctct ggcgctttca accacatggc gatcaagcta
     6301 aggatgccga tcgcccagcc cagtgggcca gtttcaccac ccttttcaaa tatcttccac
     6361 gcagtgaaga ccatgagcac agatatggcc ggggcgagac cttctacaaa atgaccaacc
     6421 caagcctcgc gtctcaaacg gtggaggatc attgttaccc ccagaatgat gagggtgggt
     6481 ggcaatatgg atgcaagcaa tgccaccaga cctcccggta ttcccccggc agcgtagcca
     6541 atatacatgg caagctgcaa agcatcactg ccgggcagaa ccgaggaaaa ccccacagct
     6601 tgtacaaatt ggctttctgt aacgaaacgc cccacagctt cgtgatagag caaccctacg
     6661 gaggctgggc cggaggtgct aagcaggttg actttgatga atgtccagaa aagttcggtg
     6721 aggatgttca aggtgattgg gctttagctg aaatattaaa gtaaggaatt ttccgaagtg
     6781 tatcatatca ggcgatgacc gcaccttcga gtttcaacag ccactccttg gtggggattc
     6841 catccccgcc gccatagccg tggagttttc catccttgcc gatgacacgg tggcagggaa
     6901 tgacaagcgg catgggattg gtggcattgg cgcgccccac ggcgcggctg gcttttggat
     6961 gacctatctg catggcaata tcggcatagg tgagcgtttc gccgtagggg atctgataaa
     7021 cggctttcaa ggctttgcgc tgaaagggtt tcaaggttcc ccagttaatt tcgaaggtga
     7081 aatcatgcct tttgcctttc aggtattccc caagttcttt ggtgtagcgg tgggttgctt
     7141 tctggctgtg ctccacggtc cctttcaggc ggaagaggta ttcaaacagc ctgggctgag
     7201 actctgccca ttcaacagca accagaccca ggtcggaggc cgcgatgcgc aaatccccga
     7261 gaggtgtgtg attcaattcg ccaagataaa ttttgagagg caggtttgtc atcgcgttgt
     7321 cctccgaaaa atattgagaa tgtttgcatt cctgtaattt gtcaaacaat cgtagggtaa
     7381 gcgtaggtca ggcgtcaagt agtttttgga caagcatgat aaatttacgt tcgtaaggtg
     7441 atttctcttc tcctccttta ccaagagaac cgcggtcggc gtccgcggtt ctcgtattta
     7501 acgagatagt ggcagtataa tggttttatc aaactcctgc gaggtatata ttatgcccac
     7561 acaaatcaca attatcggcc ttggtcagat cggtacttcg atgggaatgg cgcttgccgc
     7621 ccataaagcc tccattgtgc gggtggggca cgataaaaag gcggagttgg agcgtgatgc
     7681 ccagcaaaag gatgcgatcg ataaggcgga gcataacctg cccaccgccg ttcgcgatgc
     7741 acggatcgtg gtgttgacag tgcctgtcag tcaggtgcgt gataccctcg aatttattgc
     7801 gccggatttg aaagagggga cagtggtttt ggatacctcc cccatcaaag ccaatgtagc
     7861 caaatgggca aaggaaattc tgccggcaga ccgccactac gtgggattgg tgcccgcgat
     7921 caaccctgtt tacatccatg attttcagtt cggtcaggct gcggccaagc ctgatctgtt
     7981 tgcgaatggc cttttcctca ttgatgcacc ctacggaacg cccgaatcgg cagtgacgac
     8041 cgcagtggat ttcgtccgct tgttggaggc gttgcccatg ctcaccgacc cagccgaatc
     8101 ggatggattg atgtccacca cgcacctttt gccccagatc gtggctgctt cacttttgaa
     8161 cgccacagtg gatcagcccg gctggcagga agcacgcaaa gtggcggggc gcccctttgc
     8221 cacagtaacc gctgggttgg cttatcaaga tgagatcgat tctttgcgtc tttcctccct
     8281 gcaaaatcga gcagggatga tccatgcact tgatgtgatg atcgcggcgt tgcgtggttt
     8341 tcgtgaggat attgaaaatg aagatgatga gggtgtggcg gcgcggcttg aggcggcttt
     8401 ggaaggccgt cagcgctggc tcagtgaaag agtcgctgct gattggtcgg gtgctacccg
     8461 ctctgaagcg gctgatatgc ccacgttttc cgagcgattc tttggcggca tgttcgtgaa
     8521 aaaacacaac cggaaatgac ctgctattgc tacatcgtag aatgttctga tggaacttac
     8581 tacactggct ggaccaccga ccctgagcgg cgcgtatccc aacataacaa aggcattggc
     8641 gcaaaatata caagcacccg ccgccctgtg aaactggttt acctcgaaat acagccagac
     8701 cgcaccgcag ccatgaagcg cgaacttgcc atcaaacgct tgaagcgcga acaaaaagca
     8761 aggctgattg caggggattt gaaaccgaaa tgaacactta aacaaaaacg gcactgggaa
     8821 aacttcccag tgccgttttt gaataaatca aacttaccca gtgaacagcg gcaaatgccc
     8881 cagcgagatg atgtgcatcc attgggcacg gtcaccttcg gtaaggatgg cggcttcagc
     8941 caccaccttt gcgtcagtct tatccaggat catgcgcatc ccttgcaggg tggaacccgt
     9001 gctgatgaca tcatccagga tcacgacctt ctttcccgca atgatggagc ggtctttttc
     9061 atccagaatc aaaacctgcg gctggccggt ggtaattgaa agtgtttcag cttcgagtgc
     9121 tgcgcccatg taaggcttgt aggtcttgcg taaaatgaca tacggtttct tcgtttcaac
     9181 agacaacgca tatgccagcg gaatcgattt ggcctccgca gtgacaagga tatcgtaatc
     9241 aaccgcctga agtttctttc caagttcccg cgcacaggcc tgcaccaatt cagtatcgcc
     9301 aaggatgttc aaaatggcaa tgcgcaaacc aggctttacc tcaaaaaggg gcaattcacg
     9361 cttgatcccc gcaatctcaa tagggtggac ttgatgtttg gtcatgagtg ttcctctgat
     9421 atgaaaggta atttacccgc gaagtttatc ataactcgat attgaaaatg aaatgaatgg
     9481 ccatagaaaa tttgtgctat actctggcca tgcccatcat ccgtgcttcc gatattggaa
     9541 actacctgta ttgccgccgc tcctggtggt atcgcaaaca gggattcgaa tctgcaaata
     9601 aagctgaatt ggccgcaggg actgaactcc attctaggca tggtcgtcaa gcccttgcct
     9661 cgtctctttc ccggaccgtt ggcctgattc tgcttttagg ggctttgatc ctgctggtgg
     9721 cttattgcac cgcccagtta taaattgctt ggagaatcca tttcatgtcc gctgttttaa
     9781 gccgccttgc ccacctgcaa acccgccgcg atcgcacccc aaaccttgac cttgcacgcg
     9841 atcttgcagc gcggaacgac aaggcaggga ttcgagaaat cgcccaaaac atgtggagcg
     9901 caaacaaaaa cgttcactgg gattgcatcc acgtgatgta tgagatcggc gcaattgacc
     9961 ctgctctcat cgccccctac gccgaagatt ttgtcaaatt actgaaaagc aaacacaaca
    10021 acatggtctg gggtgccatg acggcactcg ctgaagtagc caaagtaaaa cccgaagtca
    10081 tcttcaagca tttggatgag atcaagaaag ccaaagagaa tggttcggtc attaccgtgg
    10141 ataattccat ttcggtcctg gcgcaaactg ccgccgcaaa tgaagagtac aacaaagcca
    10201 ttttcccata tttattgaaa cacctctcag cctgtcgtcc cggggaagta ccccaacacg
    10261 ctgaacgaac cctgcccgcc gtcaatgaat cgaacaggga cgatttcatt aaaaccctca
    10321 accgcagaaa cgatgacctt tccggggcgg cgtttacccg tctgggtcgc attattcgta
    10381 tcgccgagaa catctaaccg ccccttatcc ttcccatgct ttatcttgcc ctggctctct
    10441 tcctctttgc acttttcttt ttccgcaaat catcggttca gcggaaggaa gcagggctgc
    10501 ctggaggccg catcatctac acagatacac gtggatgggg caaagtggaa aagccgcttt
    10561 tttatcccgc acttgagttg actggcaagc ctgattacct tgttgaaaag aacggacaga
    10621 tcattcctgt tgaagtgaaa tcggggcgtg ccccgcaggc gccttacgac tctcacatct
    10681 accaattggc tgcctactgc ctgttggtgg aaaagactta cggaaaacgt ccgccctatg
    10741 ggatcatcca ctatgagaat cgtgatttcg ccgtagatta cacccatgaa ttggaaaccg
    10801 ccctgatcga tcttctggct gacatgaaac tcgatgaaca caagaatgac gttccgcgtt
    10861 cacatgaaca ggccgcccgc tgtgccaaat gtggtttcag gaatatgtgt gatcaaagcc
    10921 ttgcctgaag gcggggatgc gctgagacat tccaaaggta taatttcgtt atgcccgatc
    10981 ccttccttcc aaaaaatcct gttgtcacgg cgtaccccaa aatgccggaa gcattcaccg
    11041 aagcggaagc catggtgtcc tttctcaaag agagaggcat tcaagcgccg tttggttcct
    11101 tgtatgatga agaattgcgc aggcgtgtca aaaatggcga attcgacatg ctcatcgccg
    11161 ttggtggaga tggaagtgtc ttgcgggcgg ggcatttatg cgcgccctgc ggcgtaccca
    11221 ttctcggtgt aaaccttggg cggcttggct ttttgattca gatcgaccgc aaggaatggc
    11281 gtgagtactt tgaaaagttg ttcgaaggcg aagcatggat cgaaaatcgt atgatgctgc
    11341 gggccgagca tatccgtgcg ggtgaaagca tcaaccgctc caatgctttg aatgaggttg
    11401 tcgtcgcccg tgggcagaac ctgcgcccgg tgcgcctgac cgcttcggtg gataatcgcc
    11461 gacttaccag ttatgttgcc gatggtttga tcgcatccac agcgacaggc tcaaccgcat
    11521 atgcacttgc cgctggcggg cccatcctgc cgccggagtt gcgcaacatt ttgctcgtgc
    11581 ccattgcccc gcacctctca gtggaccgtg ccgtggtttt atcggaaggt tcttccgtta
    11641 gcattgttgt aaaaagtgaa aattcagttc taagtgtgga tggtcaaccc ccaacgcctt
    11701 tgatggagga tgaccaggta aaagtaaccg ctgcagatgt caccgcgcag ttcgttcgct
    11761 ttggagaccc ggggtatttc taccgcaacc tcactgccca tatgaacgaa aactcgttag
    11821 gatttccacg atgaccgata cagagatcac cccaactgcc acctattgtt atgcccaccc
    11881 cgggcgtgag accagcctgc gctgcaagcg ttgtgaaaaa cccatttgtg cctcctgcgc
    11941 ccaacgtaca cctaccgggt acatgtgcaa ggattgtgtg aaccagcatc aaaagatctt
    12001 tgacacggcg gtctggtatg actacctgac cgtgttcttc acctctgcga ttctttcagg
    12061 gctggcggcg gtcgccacca tcatcatcac atccattatt tggggctttt tcatcattgg
    12121 tctggctcca ctggcaggca cgatgatcgc caatgtcaca cggcgttttg tgaaaaacca
    12181 tcgctctccc atgttgaata ccacactggt ggtgggcatg atccttggcg cactgcctgt
    12241 tttgttgttg agcggcctgc ccggcttgct gaccttgctt ggcggtggcg gcaatgtcat
    12301 ggggtcagtt gtatcttttt cgccagtact ctggcagatc gtttaccttg tcatcgctgc
    12361 gccggtggcg tattcccaat tctctggttt ggtctttaga cgataactta tgcttaccga
    12421 actccatatt cagaactttg ccattatcga taaacttgac ctgcgctttg acaagggact
    12481 tatcatcctc acaggcgaaa cgggtgcggg gaaatccatc atccttgatg cagttgtgat
    12541 gctcatcggc ggcaaggccg atgcgacctt cgtgcgctcc gattcagacg ccgcatttgt
    12601 tgaagcggtg ttccagcttt caggtccaga aaaagaagcg gtgaccgagg tcctgactcg
    12661 cgaagaattg ctcgacgatc caaattatgt ggtgctgatg cgcgaagtcc gcaaggaggg
    12721 gcgcagtgtg gcgcgtgtga atggccgtac ggtcaatgtg ggtttgttga aggaattggg
    12781 cgccctgctg atcgacatcc acgggcaggc cgaacatctt tccctgctcg atccacgcgc
    12841 acaccttggc ctgctcgatc gctacgccga agttgtaaag cccctcggcg attaccgcca
    12901 gagctatcag gctttgcttg ggctgcgacg cgaactggtg gagctgcgca aggcgcaatc
    12961 agattctgag cgccgcaccg agatgctgac ctatcaggcc gaagaaatcg aagacgcccg
    13021 cctgaaagcg ggtgaagacg aagagttgcg caaggaacgt gaccgccttg ccaacgctga
    13081 atcgcttgcg caaaatgcac aggaagcatt ggcgattctt gatgaaggct cccccgatat
    13141 gcccgcagca accgacctga tcggtcaggc tgcgcaggcg ctggccgccc tcgcaaaaat
    13201 agatcctgaa caagtggagc tttcgaacca ggccgagttg atgctcgata cgatttccga
    13261 tgttgtacat gggttgcaga attatctgga agagatcgaa tttaatccga aacgacttga
    13321 tgaagtggag gaacgactca acctgatcca ctctctcacg cgcaagtatg gcggcagtat
    13381 ttcatctgtg ctggcttatg gagccgatgc ccgcaaacaa cttgaaaccc ttacaaatgc
    13441 cgccgaccgc atcaatgaat tggaaatgca ggaagcgaaa ctgctcgata aactggccaa
    13501 attgggcggt gtgctttctg aaaaacgcaa atccgccgca ttgacaatga gcaaaggcat
    13561 cgaagccgaa ttgggtgatc tccgaatgga agccgcccgt tttagcgtgg attttcagac
    13621 caagcctgat gtcaacggca ttccccttgc tgatggcacg cgcatcgctt tcgaccaaaa
    13681 tggtttcgat cgcgtagagt tcctgatcgc acccaacccc ggtgaaggac tcaagcctct
    13741 tgccaagatc gcctcaggcg gtgagacctc ccgtttgatg cttggtttga aaaatgtact
    13801 ggctcgcgcc gaccaggttc cctctctcat ctttgatgaa attgaccagg gcattggcgg
    13861 ccgggtgggc atggtggttg gtcaaaaact atggaatctt tcgcgctcgc atcaggtctt
    13921 ttgtgttaca caccttccac aacttgctgt ttttggcgac cagcattatc aggttcaaaa
    13981 actggtggat cagggtcgta ccctcacccg tgtggaaaga ctcgaaggcg aaccgcgcct
    14041 gcttgaactt tcgcaaatgc ttggtgaagt cggcgaagga acgctgcgtt ctgcacatga
    14101 attgctgcaa acggcgcggc agatggtcaa aaccccgaag aagtaattcg atcataaaaa
    14161 cgaaaacatc ccgcaagttt ggttcggatt tccgaaagtc aaaacttgtg ggatgttttt
    14221 ttgtttataa aattaaatga tgagcgccag ggtgaggggg gcaccctggc gctcatcgtt
    14281 ggtaataata atcgcggttc tatttttttg caagactttt ttattagagt ttccttaatg
    14341 ttgtgccccg agtaggaatc gaacctacat ctaagcctta ggagtgcctt gttctatccg
    14401 ttgaactatc gaggcgatga ctggattata cacaaaaggt taaaatagtt ccatgcgcca
    14461 tatcattctt ctccttgtcc tgcttctcac ggcatgttcg tcatccacgg cacaaccaat
    14521 catggctaca ttgcctgttg aaacctccac acccatcccg ccagccactg agagtcccac
    14581 ggcaaccaat acccccgttc caatcccgga gcgggaaaaa tatatcatca acaccttcct
    14641 tgattacgat caacatttta ttgacgtgga tcaagacatc atctacccca atcacacagg
    14701 ccagcagctc accacattga ccctggctgt gcctccaaat ttctggccga attgctttta
    14761 tctgatggaa ttacaagtgg atgggaatgt cgtcacgaat tactccttca acctgcacag
    14821 actcgacatt ccgcttccca ccccgcttga gcctgattct gtttcgacga tttccctgcg
    14881 ctacagcatc ttcctcccgt tcatggacca ggtcaacagc cagcgcgcac gcatcttcgg
    14941 ctacagcgac ctccagatga accttgtcaa ttggtatcca ttcgttgtgc ccttcaagga
    15001 tggtgaatgg atcatccgtg aaccgtggtc acatggcgaa tacctcgtgt atcccatggc
    15061 tgatttcgaa gtgaacctga aatttgccaa cccagacact gccccaatca ttgccgccag
    15121 cggacccgcc gaaccgaacg gcgaattcac acgctacacc cttaccggcg gacgtgcctt
    15181 cgcgatatcg gccagccgcg attttctagt ttccagcacg caggtcggtg aaacggttgt
    15241 gtacagttat caccttccca tttaccaggt tgcaggcgaa gccgccatgt tgaccagcgc
    15301 acaggccatt caggtctatt cgcagatgtt cggaccgtat ccacacaaga ccctttccgt
    15361 ggtggttgcc gattttcaag acagcatgga attttccgcg ttgtatttcc acagccgcag
    15421 tttctacaac ctgtatgatg gaaccccgca aagttacctc gtctcggttg ccgcacatga
    15481 aacgggacat caatggtggt tcgatcaggt cgcaaatgac caggcccgcg aaccctggct
    15541 ggatgaatcg ctcaccacct actcggaagc catctactat gaagccgcac atccagagtc
    15601 tctggcctgg tggtggacga atcgcataga tttcttccag ccttttggcg atattgacat
    15661 ccccgtttat gaagggcagg atgctgatac ctacaaatac attgtctatt tcaacggcgc
    15721 tcactttatg cgcgacctgc gtggacgcat cggcgacgca gccttctttg cctttttaca
    15781 ggattactac aaccaggggc gcggcaaaat cgtaacaggc gatgacttct tccgcatcct
    15841 cgatcagcac accgatatgg attattccga tcttgtgagc acatacttca aaaatagata
    15901 aggagccttc tccatgaaat ccgacgttat caaacccatg ttgatcatca tggcagcgcc
    15961 gatcttttcc gctctgctct ctgtcttcaa aatcgtccct gctcccccat accagggatt
    16021 tgcctccctg ctatatttgc cgatctgtgc cctgagcctg cctgttgcca ttttacttgg
    16081 gctgcagaaa aaactgcttg gtgtcaacct gctggtcggg ttgatcgttt cttttatatt
    16141 cccatttgcc ctctttattc ttatcaaccc gttatggatg ccaagtggaa tgtccaattg
    16201 taaggaagga accccgcctt ctgccacggt cgcacattat gagtgtgaag actcttccag
    16261 tgatgatggc tcctatcacc gcgattttat aatcgaagga tacaaggggt ggccgttaat
    16321 gcgtgtgatc gaggataaat aacctgtatg caagcaaacg accctgctgc ttttgccacg
    16381 ttctacggca ggagcagcag ggtcgggaat ttttcctgcc tgaaaactat cctcccaaat
    16441 acttcacaaa aaaatccacc acccgttgac tgatctctcg tcgcgatggg ctgatgccgc
    16501 ctggggcggg ggtaaatccg tgggctgcgt ttgcaacaat gaccaattct gctgggacac
    16561 ccgccgcttg aagacgcgca agcaggatct gggattgttc aagcggtacc agttcatcct
    16621 ccgtgccatg cagaatcaaa aatggaggat catctgctga aacataagta accgggctgg
    16681 cccgtgctgc atcgaaccca tcgaacacac ggcgcgccga ttcgtatcca ccctcgaaag
    16741 gcacagtcag gtcagcgggg ccaaacatat ccaccacagc ctgcacacgg ctggattgat
    16801 ccagatactc gcccacatcg aaccctgcat ttttatctgt cgtaccgagc atggccgcca
    16861 aatgtccgcc tgcactcccg ccccacacac cgatgcggtc tgggtcaagg ttgtattcat
    16921 cggcatgggc gcgtaacgaa cgcaccgcgc atttcacgtc ttcgatcatt gcagggaaga
    16981 gattctgagg cgcgagacga tagttcactg ccaccaccaa aaaccccgcc gcccgcaagg
    17041 gtccgatctc cttcgcgcct gcaccatcgg ccttatcgcc atttgaccaa cccccgccat
    17101 gaatgtacat cgccgccgcc cacggcccat cattcgaaga tggatagtac acatccatct
    17161 tcagctcgat cccattcatt atgcaatagg ttacatccct ttcgaccacg ccaagcttct
    17221 gcgagtcaaa tgcgctggat gaaacccccg tttcctcttt gacctgtgga ctgggtgccg
    17281 tttcaacagg cccaaccgcc gctgacggtt gactctgcga ggcgccacag gccagtgcag
    17341 aaattaccag taacgtgata aagaaagaaa ctcgttgcat gatcatcctt tgtgtgcttg
    17401 aaaataaagt gacagtcatc gcttatgacc gattatttta tgtattcttg tgctgcggcg
    17461 gtttgaaatt cgatccctgc aagtgactta ttccgccatt tccagaatat cttctgatgt
    17521 gaccacatgc agatgtgcgg cggcatatgc gaccattttc tcatacgccg cgaaaatggc
    17581 ctgctgatcc tcttctgtgc ggagcatgga tgggtctggg gcgtttaaat cgtagggcgg
    17641 cgaaagcggt tggacctttt tgttgttttg catcgaaaag taaatggatg tccaggcttc
    17701 tgctcccgag cggtagaaat tattttcatg aatcagcgac gttatgaaag gctcacggtt
    17761 gtacccgtgc gcatcccatt ctgacagttg ggtttgcagc attttcaagg ggtcgtacag
    17821 atcggcattg ggtttggaca tgaaattcca ccagaaatta tcggcgccat caatgaccgt
    17881 ggtgcgggtg atgctgaaat cgctggggcg caccaacaac ccgttgacgt attcaaacgg
    17941 ttgttcaaga tcggtccctg attcgtgata aagcaaagtc atttgtgcgc cgaggctggc
    18001 gtagactttc tgccccgcat ccttgatcct tccgctgttg ggcacagggg caacgacagg
    18061 attccgcccg aagacctgtg ccacgtaggt gtaaccgccg gggcgggacc tgtccaattc
    18121 accggtttcg aggtttaagg cgtaggtttc ataatcaagc agggtttgat acaactcggc
    18181 gtcgctcatg gattgcaggc ctgcatcaaa gccgttgtaa agcgggtgtg gcggacggac
    18241 atggtagctg atggtcatgt tcgaattttt gaatcgctcg atgacatcgg ggcgtttttc
    18301 ggcaagctga cgggtgatct cggcagtgaa gtagaaatcg ccgcggatgt tgtattgctc
    18361 aaagaggtca acgagtttga gcagaagctc cgcactttct tccgggtgat tccaatcgtg
    18421 gacgttgatg atgaaggaga tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnctccgcac tttcttccgg gtgattccaa tcgtggacgt tgatgatgaa ggagatggag
    18601 gcgggcgatg ttattggcga ttgaatgctg aaggtgggag atgattcggt aactgtggca
    18661 gttgtcggcg ggggcggagg cgatgtgggg gtgcaggaaa ctgcgaacag ggtgatcaaa
    18721 atcaaagtgc ggatgagtcg catttcggag tcctttcgta aaaaaggtgc gttgaagaga
    18781 gcggggcgga atttgccacg ctggtgcaaa atgaattttg ccgtacgatt ttatggtgtg
    18841 aggttgaagg tcctgacgag gaagacggcc atttcggcgc ggctcacaag ttgagtcgga
    18901 cagtaattgc cgttgccgca cccggcggtg atgccttcgt tggcaagttg ttcgatccat
    18961 gcggcggccc agtaatcggc agggacatcg ctgaagactc cgctggcggc gggcggggtg
    19021 taggctgagc catattttgc gcgcaggagg aagacggcca tttggtcgcg ggtgacgtag
    19081 tcttcaggac agtagttgcc attgccgcac ccggcggtga tgccgtcgga ggcgagttgt
    19141 tcgatccatg cggcggccca gtagtcggca gggacatcgc tgaagaccgt gccgctggcg
    19201 gtgggcgggt tgtaggcggc gccgttcatg ccgcgttcga ggaagatgga catttgcgcg
    19261 cgtgtgaccg gggcttcggg gcagaaggta agcgggttcg ttccgcagcc gccggtgatg
    19321 cccgaggcgt agagggtttc gatccaggtt ctggcccaat aatcggcggg gacatcggcg
    19381 aagagttggc tggtgtattc atcggcgccg atatcgcagc ccgcaccctg cggacgaaga
    19441 tcaccggcga agtcatcggt gggagcgttg gcgcagttgg caatgtctat ggcgggtgag
    19501 gtggaaagca ggtggtaatc gaaaaccgtg gtgccggtca attgcgggtc ggtgaacagg
    19561 gaatgggcgt cattgccggt gcctgcctga taggcggcga agccctgata aaaaacattc
    19621 ttccattgcc attcactgcc atcgccgcct gcgggggcaa agtacaggtt gtaatccacc
    19681 acgttgtttt gattttgggt gtaggggttg gtgataagcc agctttgcga attggcgaaa
    19741 acgatgttgt tctgaatggt attgttgttg acgttgtatt gcagccagat ctcgccgttc
    19801 ccatcttgat tctggtcgtt ttcgaagaag gtgttgttga tgatggtgga attctcagtg
    19861 ctgccgcgct gggtgtcgta accgcccatg gcaagcccgg caatgtggtt gtaggatatc
    19921 aggttgtttt gcacggtgat gtagctggtg gcatggccct gatgttcgct ggcaagttcg
    19981 atgccgatgt tcgaggcggc gatcttgttg cgttcaatga ggatgcgtgt gccgccatcc
    20041 acatagatgc ctgctgcgct ttgtgagccg ccgtaggccg ggttggtgat cgtgtcaatg
    20101 gcggtgacgg tgttatcgct gacgatgcca tcccgtgctt gatcgtagga tgggttgggt
    20161 gaaacgcctt caaagccgat gaggtcaatg ccgatgttgt cgcagaaggt cactgtgttt
    20221 tgggtgacga tgaaattttc cacgttgccg ttgaggacca gcgcttcact gctgccgagc
    20281 ttgaggtcac ggatctggtt gccttgaatt accaggtcgt ggatcgaggc tggggcggcg
    20341 gtgccataga cggcgattcc gtgtgcgttg ccattggcgc ctgcattggt ttcgatgtgg
    20401 tggacgtaat tgttgaggat ctgaatatga tgcgatgcgc cggtgacaaa aatgcccatc
    20461 ggtgtggcgc ctgcctgatt ggttttgtag ttgcggatct cgaacccgtt cacgctgata
    20521 tggctttgat tctcaatggt gaaggcgata tccccggcag cggaggaaaa tccgctgcca
    20581 tccagaatgg cggtttcgcc ggaatagttt tgaaaggtga tgtagccgcc tgcatccgaa
    20641 ccagagacgt taatggagac cgtttcattg taagttccac cgcgtacctg cactgtatca
    20701 cccgcgctga cggaatccgc tgcgtgttgg atggtttgcc atggctggtc gagtgtgccg
    20761 gggttggcgt cgctgccatt gacggcaacg taataggtca cccctgcggc gcggaccggg
    20821 ctgacgggcg cggcgctgaa aaagccaagg gtcaatgcca gagcaaagag cagggtaagg
    20881 gtgatttgaa tgaaacggcg catgtgagat tcctttcgcg tgcaattgtt gtgcgtggat
    20941 tctaacaggc ttgggttgcg ttagcggacc gtgaatgtgt acacatgtgc cgtggccagg
    21001 tagccgacat ggatctgatt ccgtgcgggg ttgtaggcga gggtgggtaa aaaggatgct
    21061 gattcgggga gcggctggct gaagcgcacc gacagagtct cacggtctac tgcaaggatg
    21121 gcattgctgg cagaatcaaa cacccagtat gtgttcgaag cggaatccat gccgaagatc
    21181 ttgtcgcctg cgctcagtgt gcgtccgctg ggctggcctg tgaccgcatc caattcaagt
    21241 gcgccgaggt agatgcgatt ggttcgggca tcgaagaaaa acaggtcggc acctgattgg
    21301 ccgatggagc gggacgcttc gcgcaagtcg acactgccaa ggggagagaa ttgatttgcg
    21361 gcatcgtaga tccacagtcg gcgggatttt gaatcgtaga cgaagacacg attctccgtt
    21421 tcgttgacag tcacctgcag ttgaccgggt cctccgccgg tgtcgcctgt ttctgcccct
    21481 tcaacaggga tcgtggcgac aggctgcatc gtttcccagt tgaccacggc aatggtgccg
    21541 aattccgggt aggcggcata ggcgagcttg tgggtcgagt cgatggcaag atcgggcagg
    21601 cggtcggtgg aacctgctgg ggttccaagt ggaattgtgc caagcagggt ttgatttggg
    21661 gtgatcgagt aaacagagag ggttccatcc caggcattca gtgtgaagag gtgttcgccg
    21721 ctggcatcgc gtcggatggg gatcggccat gtgcccgcag tgaaggcttc aaatgcaccg
    21781 ctatcttgat tgtagctgcc gatgtaactg ccgcccaagc ggctggcaaa atacagggtg
    21841 ctgccatcgc cggatgagac gatctgctcc acgctgtcgc cgaggcgcag ggaggtggct
    21901 tgcgtgggcg catgtgtatc cacgctccag accaccgagg catcgccgtt cgggatgtag
    21961 atatgcccgt tggctgggtt gaggttcatg tggtgcggtt ttcgtccaaa atcgaagctg
    22021 gaggtccggt ttcctgaaag gtcgtacaca tccatgtggc cgccgatgcc tgccacctgt
    22081 ccgttttgcg gggagttgat ggaggagacg aagacctgtt ttgtggcggg gtcataaacc
    22141 tgatcttcaa cgatgtgttc accggaaagc gggaaggcgc tgatctcagt cccattcata
    22201 tcgaagatgg cgacgctgcg atccatgcca atgatgaagt gttggtcggc tgggtcgaag
    22261 tgcagccagc gcgagcggat gccctgcatc gcggtgtagt ggatgctccc ggtcacttct
    22321 ccgcttgcgg cggcaaggat gaaaatgttg cggtcggctt ccttgctggt ggtatcgagc
    22381 aggtagaaga catctttggc tgggtcatac accatgggac cggcactctc gccgagtccg
    22441 cttacagtgt agagttgttc gagggtgttg ccatcgaaag caacgaggcc cttggaggtg
    22501 gtgagataga agcgggtatg agtttcatcc actgtggcaa gctggccgaa gccgccctcc
    22561 gccaatggaa cagggccgac gatttcgccg ctttccacat ctacacggcg caattccttg
    22621 cggaccgtat cgtagatgta aaggtattgc gcttttgaat caaagtagat gtatttgatt
    22681 cccctttcag tgagacccgt ttccacggtg ccgatccatg tttcggttgc gccgtcgagt
    22741 acggccacat cgagggtcat gatgcccgcc acatatacgc ggttgttttc atcatccact
    22801 gcggcggaga atagcttgag cacgttttcg tccacgctgc ggttttgcag atcaagcagg
    22861 gatatggaag cggtgaggca gatcggttcg acacaatcgg cttgaacggt ggggacgatt
    22921 tgcggggcgg gaatgcctgt tgaaacagaa tcagtttcgg gcgcgggttg aggcgcctgt
    22981 ttgggaggct tccccggcat gcaggccgtc atgatgaagg ccaggagcag gatcaggttt
    23041 tgaaattttt tcatggtggt ctccgtgttt tattgttttt cgtgcaaaga aaaaatgaac
    23101 cgcgaagctc gcaaagatcg cgaagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnntcgc aaagatcgcg aagataagtt tttagaatct ggcgctctta atgcatttcg
    23281 gcgtaggcat cggccatttc agaaaaggtg gcccattgca cctgtccgct ggcgacgagc
    23341 gggtccacta cttcgctcag gaatttttcg atcacttcga agggatgctg cgggtcgccg
    23401 cgaaattcgc cggggtgaat ggtgaaatgg aaaacgttca cttcgcccgc ttcggcggca
    23461 tcgagcgagg catacaaact ttgcttgaga aattcaaagt aggcttcgtc gccgcctgta
    23521 tcttcgctgc ggcgcatcga ggcaaaattg gtcttgtcat actggccttc aggcagaaag
    23581 atgatcgcgc catgggggtc gtgccgggtg aagaggttga agtctgtgcc gtcggtgccg
    23641 cctgcgggac gccacgggtt cacacctaca aggtcgagcg gcgtttcctg tgtcatgcga
    23701 ttcttccagt cactgttcac gctcagcccg gcacattcgg cggcttcgta caaattcggg
    23761 ttgagattcc ctccgctcca aaagcggatt ttctccacgc tggcggcttg ttcaatgagt
    23821 tcaatttcct cgctcatcac atcgcaccat gtttgcacgg tgagggtctc gctgtttttg
    23881 ccgaggtgcg catcttcatg gaagtgcagc ccgatctcat gtccgctggc tgcgaggtcg
    23941 gcgaggatgg aattacccga ttcgatcgcc accgtggtga acggcgattg agcctgaatg
    24001 gtcatacgcc cgccatgcgc ttcgacgatc tgcgtcacag tgcgaatatc ctgcacctgc
    24061 ttatcgaaaa aaacggggtt gtggtgatcg cccttgcccg atttgtatcc ctgcgcggtt
    24121 tcacccatcg gttcgatgtg cattccgatg gtgaacagca ggatggcttc gcccgattct
    24181 gactcagccc cgggctgggt ggcggtttca agcggcagag tctcagcctg ggcagtgagg
    24241 ctgcccggca ggttgcaggc cgtggaaaga acgaccaata ggatcaaaag gtgggaaatg
    24301 cgtttcatgg tttgcgctcc tttcgcttgt actatacagt ttcaccatga ccgcacagtt
    24361 ttcccccggt aaaatgattg taaaaaaagc cagtcacctg acagtttgtt tttgggagcg
    24421 gctaaaagaa ggtttggtaa tgggtttgtg taaatgatgt agcgcgagat actatctcgc
    24481 gctacggaca ttgtcattta tttcgaccca tcaccaattc tttttagaaa aaacaacaca
    24541 gggtcacaaa ggaaatttcc cttgcaaccc tgtgtcgcgg ctttgtgatg cccccgctca
    24601 aacttccgtg gtcatctgcg gatggggcga tctattttat cgaacgttgc gccgtacacg
    24661 tagggattgc ccggaataaa atcatacggg aaccccattt cgatggcgct gactttttca
    24721 agccgctggt attggtcatc gtccaatttc caatcgaaag cggcaaggtt atccttcaac
    24781 tgactggccg tacgcgctcc aagaatggga atcatctgcg ccttcggaga ttgccttacc
    24841 caattgatcg ccacttgcga catgggcttg ccagtctctt ccgaaatccg ctgcacttcc
    24901 tgcaccacgc ttaattgctt ttcgctcaag cgaagttttt tcggattcaa ccgcgtggag
    24961 tttttgatgc tcttcaaaaa ttttcccgtc agaatccccg cttcgagcag gccccacggc
    25021 agcaccgcca tctcccaatg agccgccatc ggaatcaccg acctttccac ggcacggtcc
    25081 agcagtgaat agggaatctg cattccaata aaacgcgacc accccatcaa ctcggcgcgg
    25141 gtgttggcct ccgccacaat gtaatcgggc gaatcagaaa aagccacata attcaccttg
    25201 ccctgccgca ccaggtcatc gagtccgcgc accacttcat ccactggcgt catgtaatcc
    25261 cacacatgca aataataaat atcgaggaaa tcggtcttca accgcttcag gctttgttcc
    25321 accgacctca tcatgttctt gcggctgttc ccgccgccgt tcgggtcggt gttgtcgggt
    25381 ttggtcagcg tgtatttgct ggccaacaca aagtaatccc gatccgtttt cagaaaatcg
    25441 ccgagaaatt cctcggcagt cccgtctgtg taattgaacg aggtatctat gaaatttcca
    25501 cccgcctttg tgaacaggtc gaacatcttt ttgctttcag cctttgaagc gccccacccc
    25561 caggtttcac caaaagtcat cgtgccaagg ctggcttctg aaacgcgcag cccgctgcgg
    25621 ccaagtaatt gataacgcat cgcgtcctcc tgtaattttt ttcagtatag cataccgatt
    25681 tgcatacaaa attccaacat gcccgccgca gacatcatgc tacaatcggc ctgtgaacgt
    25741 aacccgacca ccctgctaag ttaaaactga atcaacggtt cacaaggccc actttcagcg
    25801 ggcccgtttt acattccccg taaccaaaca taaacccacc cccgatgatc tcgacggtgc
    25861 ctgacgtaca ggcagccgtt tcacttcacg cgcccgtgat cccccaactc caacctgtct
    25921 ctattcaacc cattagacag attggagctt tcacatggaa acaattcggg gcagactgtc
    25981 ccattttttg gattcgaccg aactacaggc gcgactcaac ccttacaacc gcctcacact
    26041 ggaccttgga accggtgacg gcaaatttgc attttgccgc gccgatgcat tccccagcca
    26101 cttcgtcatc ggcgtggact cgtgccgcga aaacctgcac gagtattcac gcgccaaact
    26161 gccaaacctg ctcttcatca tagccagcgc acagtccctg ccgcatgaat tgcacggact
    26221 cgcctcacac atcaccatca actttccgtg gggcagcctg cttgaaagtc tgttgaatgg
    26281 cgatgacaga ctcttgcatg gtttgcagtc tgtggcacgt tcgccagccg cattggatat
    26341 ccgcttgaat ggcggcgcac ttgccgagca gggctgggcc cttgaagatg gcacagaacg
    26401 catttttgag aatctcacct ccacaggttg gaagatccgc ccgcctgaaa tgctcgattc
    26461 gcacgctttg cgggcgttcc ccagtacctg ggccaaacgc cttgccttcg ggcgtgatcc
    26521 acgggcaatg caattgagcg ggtgtcttta ggaaaacaag tcataccgca agtatgattt
    26581 gtcttcttct gattcttgat ctatttcccc atctgacgca aaagacctcc tgtgtttttc
    26641 acaggaggtc ttctctttat ttgaacagaa tcagtttcag ggtgcgtttt gaaacgcggt
    26701 gtactttaat ccagcttgcc ggtgatgatg gccatcttca cttcgccgat ggctttggtg
    26761 atcttgatct cacgcgggca ggcttcggtg cagttgtacg ccgtgtgaca gcgggcaatg
    26821 ccgtctgttt cggcaaggac ctgcaaacgt ttgtctgcgg cttcatcacg gctgtcgaaa
    26881 atgaagcgat gcgcggtgac aatggccgcc gggccgtaat attcctcgtt cgcccagaac
    26941 gacgggcagg aggtggtgca ggccgcacaa agaatgcact tggtggtctc gtcgaaacgc
    27001 gcgcgctgtt ccgggctttg cagtcgttcg cgtccatttt cggggagcgg gctgtcgttg
    27061 atgaaatacg gcagcatttt tcgataatta tcgaaaaacg gttccatatc cacgatcagg
    27121 tctttcttta ccttcaagcc gagcaccggc tccacagtga tgtttgcgcc cacatcacgg
    27181 atgagggttt tgcaggccag catgttgcgg ccattgatgc gcatcgcatc cgaaccgcac
    27241 acgccatgcg cgcacgaacg acggaaggac aacgtgccat cggtcttgcc tttgatgaat
    27301 tcgagcacat ccagcacgcg gtcgttttca tcggcttcca ccttgtaagt tacgtaattg
    27361 ccgcgcttgt ccttctcagg gttgtaacgg aaaattttga ttgtaacttc catgtttacc
    27421 tctaatacac tcttgccttc ggctggtgtt tggtgatgac aacaggcttg taattgatct
    27481 caagttttcc gttcttcttt gcgatcaacg tatgcttcaa ccaattggcg tcatcgcggt
    27541 cggggaaatc ctcgcgtgaa tgtccgccgc gggattcttt gcggttcaat gcgctggccg
    27601 ccaccacttc tgcgatctcg agcatgttgc cgagttccca tgcattcagc aattcggtgt
    27661 tgaagatctt gcccgtgtct gtcacgccga tattcttgaa gcgtccctgc agttcgcgca
    27721 ctttttccaa tgcagattgc atatcttttt cgtttcgata aatgccgaca tctgcgaaca
    27781 tgaccttctt catctcgttg gcaatgtcga acgcattttc cttgccagac cccttgcgca
    27841 gcgcctccaa agcggacctt gtacctgctt cggcgtcctc gggcattttc acccattcag
    27901 cagacttggc gtattccgct gcacgcagac ctgcgtgttt gccgaacacc accaggtcga
    27961 gcaacgagtt cgtccccaaa cgatttgcgc catgcacaga gacacaggca cattcacctg
    28021 ccgcaaacaa accgggaagc gctgtacctt tgtcgtccgt caccacttca ccgaacttgt
    28081 tggtcggaat gccgcccatg gtgtaatgcg ccgtcggttg gatcggcatc acctgggtga
    28141 cagggtccac accgagatat acgcggcaga aatccacaat atctggaatt ttctgcatga
    28201 ccgtttcgcc ggtaatggtg tacggtgtac cgtctgggtt ggtgcgtccg tccaatgcgg
    28261 cgaatttatt cacgctttca gggcgaatat ccagatatac atagtttgag ccgttgatgc
    28321 cgcggccttc tttaatctca gtgtagatcg cacgcgaaac aacatcgcgt gaagcaaggt
    28381 ctttcacggt gggggcgtat ttcggcataa aacgttcgcc gttgccgttg atcagaatcc
    28441 cgccttcacc gcggacacct tctgtgatga gaataccgag tttgtaaatc cctgtcgggt
    28501 ggaattggaa aaattccatg tcttcgagcg gaatgccgtg acgcagcagg atggccgcgc
    28561 catcgccagt gtaggcatag gcattggagg tgacttcgaa gatgcggcca tggccgccgg
    28621 tggcgaagat gacagccttc gcgtggaagg tgtgcaattc accggtggca agctcaatgg
    28681 caacaacgcc cgcagctttt ccatccacca tgatgaaatc gagcacctga tattcatcga
    28741 agaaggtcac atcgttcttg atgcactgct ggtacagtgt ttgcaggatc atgtggcctg
    28801 tgcggtcggc ggcatgcgcg gcccttctca cgggtttacc ggtgacattg ttggtgtgcc
    28861 cgccgaaagg tcgctgtgag atgcggcctt caggagtgcg gtcaaagggc aggcccatgt
    28921 gttccagttc gagcacagcg tcgatcgctt cgttggtcat gaactcgatc gcgtcctggt
    28981 cgcccaggta atcggagcct ttgacagtgt cgtacatgtg ccattcgggc ttgtcttctt
    29041 cgtaattgcc cagcgccgca gaaatgccgc cctgtgccgc accggtgtgc gagcgggtgg
    29101 ggtacagctt gctgataaca gcggtcttgg caccgcgcga agcatacaga cctgccatga
    29161 ggcctgcacc gcctgcgcca accacaacca cttcaaattg atgaacattt gccatgaatt
    29221 actcctgaga aaacctttta ggttttaacc acagataaaa gatttttcac ctgcatttat
    29281 ccgtgatgaa tctttttacg aagtccagat gagatacaag cccatgagac tcatggaacc
    29341 catcatgatg atgctgagca ggcggacgat cttgcggccg ccctcggtgg tgatgtagtc
    29401 atctgcaatg accaccagcc catgcacgcc gtgggcaact gcaacgaaca gcagcgcact
    29461 ggcggttaac ttccagaatg gggaagacca tggattgaga tcgggcacat cggtgctttg
    29521 cacgtgggtg acattgggca tgaaagccca gcgcatcaca tctgcaaagt tcatctgttc
    29581 acgtgcgccc attacaaggg cgccgatcat gccgaagatg atcagggcat acatggccag
    29641 ggcggaaaga cgcgtaaaaa tccacatgat ggattcgaag ttcgttaatc cgcgttgttt
    29701 aatggagagt gttcttgttt tcgtggtcat tctagcctcc cagtgccgtg gtcacaatga
    29761 caaaaacgga gatggcatac acaggaatga ataccgccca ctcgatccag atggcctcac
    29821 gatgatggtc gatcagtttg gggaacaggt cgaggatcgt gatccgcaag ccgttgatgg
    29881 catggaacaa aacgcaaaag aaaatgaaaa tctgaaaaac ccagttttcg taaatgctgt
    29941 tcacaaacgc tccgcctttt ccacccaaaa acgaagccag aatatgtgtg gtgatgaaga
    30001 ccgccattcc cagcccgcca atgcggtgca ggacgaacgt caacatcgga ccctgcccct
    30061 tgtacctcag tcccgtggat aggctgacat ttctcttcag gcccatctat gcctcctcaa
    30121 aagaattttt tcataagagg cctgcttgaa aatggcacaa agcccccatg atcagacaaa
    30181 tcccatgttc atgcgtttat ggtctcttat gatacaaggg cctattttac tcttttaact
    30241 ttgaaaccgc aaaatatggt gacaattgtc atccatattt catttcagct atcctataat
    30301 aaagcggaga aagaccctta taatccaatt cacttaacgc cacatcatct ttaatattcc
    30361 ctgactacag agtatcgcac gatttcccag accaaagctg tgattattac ccgtaaatcc
    30421 atgcaaaaat ctggcgatct ataaggcaag cgacagaagt tatttccaaa agtccaagga
    30481 ggcgtacatg tcaggttcga atgaactttc ccggcgcgat ttcatcaagg tcacaaccgg
    30541 gatcgtcgga ggtttaatcg gggttgcgct cgggttgcct tcggtttcgt atttaataga
    30601 tccagccctg caggaaggtg ggaaagacgc atggatttcc atcggtaagt tcgaagatat
    30661 gcagcttgaa aagccttatc cgttctcgtt tacgcgggta caggtcaatg gctgggagcg
    30721 gacctccagt tcgtttggcg ggtacgccat ccgcaaatca gatgacccca aagacctgct
    30781 catcctcaat agccgctgca cacacctgtc ttgcaatgta aactggcacg aagaatcaga
    30841 tgcatttctc tgcccctgtc atgatgccaa attcagcagg gatggaaacg tgctcgatgg
    30901 ccccccgccg cgcccgctgg atgtatatga agaattccgc ctctcagaag acggcaacat
    30961 cgaaatcttt ttcaaggcag gctaaatcat gtggaaaaca gtttacgaat ggcttgatga
    31021 acgtctcggc ttgaacgacc tttacaccaa cctgctggat cgccctgaac caaaaggcaa
    31081 ttggtggaac acccttggct cagccagcct tttcctcttt gtcctacagg gcctgaccgg
    31141 tatcttcctc accgtctatt acaccccctc ccccgaccat gcctatgatt ccgtgcaata
    31201 catcatggaa ggcgtcgctt ttggctggtt gattcgcggc attcatcatt ggggtgccac
    31261 cctgatggtg atcgtggttt tcattcacct tctgcgggtg ttcatcaccg cttctttcaa
    31321 atttccgcgt gagttgacct ggctcattgg cattggcctg ttccttgcca cacttggcat
    31381 gggcttcaca ggctacctgc tcccctggaa ccaaaaagcc tattgggcca ccacggtcgg
    31441 cacacagatc gcaggcagtg ttcccttcat cggcgaattc atcctcaaag ccctgcgcgg
    31501 cggacctgat ttgagcgcgc tcaccttgca acgcttcttc tcagcacata tctggatcat
    31561 ccccgccgta ctggctggcc tgatcggcat tcacttgtat ttgatcatca aacatggtga
    31621 atcgcacttc cccacaaagg aagattagcg attcttcccc atacaaggca tacaaccatg
    31681 aacgaagaaa ccaaaaagaa aatcaacgca cgatacgaga aggaactgaa caaaggcgaa
    31741 cgcttctggc cagacagcat tttcaaagac ctcgttgtct cgctcggtat cttcatcctg
    31801 ttggtgttac tggcaagctt tgtaggggtg gctccggaac ccaaggccga ccccagcgac
    31861 acctcctaca ttccgcgccc tgagtggtac ttcctctttc tgtttaagtt ccttgccctg
    31921 tacgggcaaa tccccctgct tggaaaaatt gaatggcttg ccactgtact tgtccccggc
    31981 attgccattg gcgtactcac cctgctcccc ttcatcgaaa aaagccctga ccgtcactac
    32041 gcaaaacgtg ccctctccat ctcggtcatg accatcatgg tcgtgggtat tgtccttctg
    32101 accctgatgt ctgaaattcc caccgtctct gccgatggat cgaaattgct tggcctgctg
    32161 caagccatgg caggactcgc cgttcccggc ctggccatga tcgccttgtt tgtcgccgct
    32221 ctcgccttca aaacgacgaa cacccgcttc ttcatctgga cgacgagcct gaccgttgtt
    32281 tcaatgatca ttctctcagg gacagtgatg gctctgcaca ctccgcccgc cgttgaagag
    32341 accgaagtcg ccaccaccct cgtagaccag atcttcgccg ggcaggacct atattccgtc
    32401 cattgcaccg aatgccacgg tgacgatggt tcggttgcgg tcattgaagg tgtggaaggt
    32461 ctcgaaggcg aaaagatcac ccccatcaac agccacgacg tactctacac catcaccgac
    32521 tctgccatgt acgaggtcat tgcctatggg cgccccaatg caggtatgac cccattcggc
    32581 aaggcgtatg gcggtgaact ctccaagagc gaaattgatt acatcgtcac ctatatgcgc
    32641 tacatgtggg atgaccgttt cgagatccct gctgaagcgc ttaaaccgtt gttccctcca
    32701 ttggcagagg gcgaagtgcc ttcgtatgaa gtccacatac agcccattgt gaagcgatat
    32761 tgtgtctcct gtcatcgtgc cggcaaggaa aacaacaact acctgatgac ctcttatgaa
    32821 gagatgctca ctacaggtga taacgctgcc aacaacatta tcgcaggtga tgaaaacagc
    32881 tacctcctgc aagtcattca ggaacatgcc atcaccgatg ccaatggaca ggaattgatc
    32941 ggtgtcatgc cgcccaaccg cgcgctcaaa cccaatatcg ttgatgtctt catccgctgg
    33001 atcatgaatg gcatgccgca gaccgccgtt gatgctgccg cgttgttcac tgcaccagcg
    33061 ccatagatgg tgtttgctaa caagaatcaa aaaagccgtg atgtaatgtc acggcttttt
    33121 tgatttaaag caaagacatt gggagtggtt tcagcctgcc accagcagga tcaccagacc
    33181 aatgatcgct ggtaaagcct ggatataaaa tatccgcttg ctgaccgtga acgcgccaaa
    33241 aagaccggcg atgatgacac aactcaggaa gaaaaccttc acggcatccc cggcgaaaat
    33301 cccccaaatt aaacctgccg agagaaaacc gttgtacagc ccctgatttg ccgccagcgt
    33361 tttggattgt gccgcaaact ctgcagtagt gcggaaggat ttacggcctg cgggtttttc
    33421 ccacagaaac atttcgagga tcaggaaata tccatgcaaa acagccacca aacccgtaat
    33481 gatgtttacg acgatattca ttgtttctcc tttaagttgt gtctgaattt gaatggatga
    33541 taccccataa aaagaaaaag gacggagtcg cagccgactc cgtcctttga ttccaacttt
    33601 tgatttactt cttcttgcca gtcttcttta caaccttctt ggttgtcttt tttgcaactg
    33661 ccttctttgc aggtttcttt gcggcagcct tcttcactgg ttttttcgca acagcctttt
    33721 tggcaacggc ttttttggcg ggtgccttcg cagcggcagc ttccgctttc ggggcgggaa
    33781 tttccttgtc catccagtga atgatggcat cgccgaattc agagcacttc acttcggtgg
    33841 gtttgtccat caggcgggca aaatcgtagg tgacggtctt ggcgtggatg gcgccttcca
    33901 tgcccttcac gatcaagtcg gcagcttcgc cccagcccat gtagcgcagc atcatttcgc
    33961 cggagagaat gaccgagccg gggttcactt tatccagatc ggcgtacttg ggcgcggtgc
    34021 cgtgagtcgc ttcgaagatg gcgtgtccgg tcacatagtt gatgttcgca cccggagcga
    34081 tgccgatacc gcccacctgc gcggcgagcg catcagagag gtagtcaccg ttcaggttca
    34141 tggtggcgat cacgtcataa tcgcgcgggc ggatgagcac gaactgcaag gtcacatcgg
    34201 cgatggagtc cttgagcatg agcttgctct tccacttgcc gtcgccgtgg gtatcccaca
    34261 actt
//
LOCUS       DAOQLC010000035        34205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_391_length_34205_cov_25.609341, whole genome shotgun sequence.
ACCESSION   DAOQLC010000035 DAOQLC010000000
VERSION     DAOQLC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34205
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_34205_cov_25.609341"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PL000_08220"
     CDS             <1..836
                     /locus_tag="PL000_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC88923.1"
                     /translation="RRAFRPYGKTAQAIWRWKRFSFNDAPPIFANSKPKSGSHLLLQV
                     MNGFTQIMPYKYVDADPVRTITYDGRRKNKEEILADLKHTPKGVIGWGYVDATEENAS
                     FLTAAGRVNYFIYRDPRDMLVSQVFFATDMHEEHGMHEYYKSLPDFAARLNVAITGID
                     RDGLKMVSVKQRYEGVFQWLEQQNTMCIRFEDLINNRDATLLKMLDEVEKTGYKIPTP
                     REKALSILVEAIQPKKSHTFRSGKTGGWKQHFTEEHKKLFKEVTGNLLVRLGYETTND
                     W"
     gene            complement(919..1248)
                     /locus_tag="PL000_08225"
     CDS             complement(919..1248)
                     /locus_tag="PL000_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88924.1"
                     /translation="MVIKAVLIGSVLGVLEWVILVGSRQKLGFWIYIQSMLAWFTCGF
                     MIGVVDSPFPAYLLGSVLAVFLNLPWYINISIIPKQYNHLPPLIISSIVLGAIGGGLK
                     ILLSAMF"
     gene            complement(1257..1580)
                     /locus_tag="PL000_08230"
     CDS             complement(1257..1580)
                     /locus_tag="PL000_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88925.1"
                     /translation="MSNQVIELASFRLADGASEATLIAASDAMQEGFLKSQKGFIKRD
                     LVKGADGQWADVVYWDSQESVDAAMQEAMNNPACFKYFELMTGVNHEDVSAGVMHLSV
                     LKSYL"
     gene            complement(1634..2539)
                     /locus_tag="PL000_08235"
     CDS             complement(1634..2539)
                     /locus_tag="PL000_08235"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNC88926.1"
                     /translation="MNRIDRLFAILLELQHKRRLRAVDLARQFEISKRTIYRDMSALN
                     QMGIPIAALPGEGFELVEGFYLPPLMFNENEAVALTLGLRLLTQQAAGSLTQAADSAS
                     AKIKVALPEQVRVKSEALSGIIGFITPSQKFNLDDSQLLLIQKAIQEKQVMHIRYHGY
                     RKDEISERDVEPHQLYYSDGVWYFEAYCRLRKDVREFRLSRVEKLTLLDQHFSKRRKS
                     KAAAARMVEVRIRFDARILRWVRERQHYGFVREKDAVMTYHVHDVSEITSWILGWGAE
                     AEVLAPKDLRKELRETAKKLANLLT"
     gene            complement(2615..4144)
                     /locus_tag="PL000_08240"
     CDS             complement(2615..4144)
                     /locus_tag="PL000_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88927.1"
                     /translation="MSTKYVLHTLTLIWFVLLISGCANPPTATQTSPSTPALPSGWEK
                     KVSNIIWIAYSPPNANPNKGIQASAEDIQADLEVLHNAGFTGLVTYDSKGVMGRDFPT
                     LAKETGFKGVIMGIWDPTSAEEMSNAQAASSNPIVLGFCVGNEGLDVRYDMTVLSSTI
                     EELRKTTGKPVTTAEQLDDYYDEKLLEIGEWVFPNAHPYFHSQIDPVNAVQWTEGAYK
                     DLSSKTDRFILFKEVGLPTEGDAENNLSETAQDQYYQGLEYTDVKFVYFEAFDQPWKT
                     NLSVEPHWGIFFSDRTPKQTAVSFMGGEPVVEFPTATATEIPVETELPISDDIFYIYN
                     DGASPYNHFSPSGYMGDTGDIQIDGASTENPQSGDTSIQVIYTPKGQGPYACAYKPPC
                     KWAGVYWQQPANNWGKEQIWAGTGFDLSAYRTLKFWARSESTCTLEFKVGGITGDYGD
                     SLVYPRGITERLTTEWQEYEIPLEGADLSYIIGGFVWSTSWEKCPGGATFYLDEIRFE
                     K"
     gene            complement(4145..5047)
                     /locus_tag="PL000_08245"
     CDS             complement(4145..5047)
                     /locus_tag="PL000_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88928.1"
                     /translation="MGRKRSTAKNSPKTTKEWAQIIVALIAAVATVSAAVIAIIPVFI
                     KDAPETATQSADAPDASSANVQDGLVGKMPPENADRFLVNRFYKPTQHMGDRGDIDIS
                     GIGDTANPIIFTYSTNGNGPHEWDYKYINKEINPNPARFAGIMFIDSAGPGGTDPNNG
                     FDLSAYKTIRWEARSVQGLVDVKFVIGGVTWTWDEQTKERVVAPYPDSVRSSVLLCEP
                     TLTQEWQSFEYDISGFPPDSFQHVVGVFGWTIDWGDNNITSITNPDSSIVPENPKTLV
                     IEIRNIYYDKAIVEHPATSDCGSK"
     gene            5256..8681
                     /locus_tag="PL000_08250"
     CDS             5256..8681
                     /locus_tag="PL000_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC88929.1"
                     /translation="MRPKLDLAGNSALVESPPRVRIWISALRYLLGKALTIFATIFAG
                     VFLTLLLANQPSPRGLDPARSPFEISLESQIARVIRISIANGTIPRLSDGYPDQHQLD
                     ALEKKLRDDAGLELPFFPRYLWWTLNALQFNWGELTVTSTETTGFSLADPVGKRDLVL
                     EYLPNTLLLIGTAFLLIFLIGMPLSLYLARNYGGWFDRLLTFLSPISSIPSWVVAMLL
                     IALFAVKLRWFPVGGKFDFATPQDPLEYILTLFRHMVLPVSAIVFSMLFQVVYAWRTF
                     FILYSEEDYVDLARAKGLPHKILERQYILKPSLPYVMTSFATIVIGFWQLTVALEAVF
                     QWPGIGWLYIREALPNFWGESMDPGQLMLVIEIVVIFAYLLGAVVFFLDLAYALVDPR
                     IHLLPSRNTTQGRVKTRMQGTDWLARFNAWMKEKTSRKPKQATGPVRPLKFIWEAFAD
                     SLHDFDARSRLFFHELRRYPSAMFGLTVISIMLVGSIYAVIGLPYEKYGMEYNTNRVT
                     GRSYTPRLAVPAWTNLFSSMPRLSTLVINEKSPRAQVSLRSLENGWVEKTITFTFDYA
                     YRETPSDIFLYLEPKYVEKFPFISLTWITPDGRDLDLVAKSINAVTNYDFETGIRVAK
                     LIRQNPEWEKWFVRSGQYPTPAINLLFATPHEPQLHLQSGQYQLVLKSLLFEDESDLT
                     AELVLLGQVYGAAGTDYWRRDLVVPLLWGMPFALIIGLLGTLVTILVAMLLPAIGVWY
                     GGWLDEFVQRLTEINMVLPGLMIAVLAYALFGINIWIVLGLVVMMNAFGSPIKTFRSA
                     LLQAREAPYIEMARSYGATDFRIITRYLVPRILPVVIPQLVSQIPGFIFLEATLGLFN
                     IKSNYPSWGRIIYDGLARNALYGSPFWVLAPISLLLLTGLAFALLGTALERVLNPRML
                     EQVSQAEEKEIAKQRRVSKRMIATITAAALLMFFILVPSIKTLSGMFNRAPSTASDRM
                     AVPVAVTLPSVTAPSPTETPDPVPQASSTLTFSPTPTLTPSPTILPSLTHTSTPLDLR
                     PKTYTLQAGEFPYCIARRFDIDPIDLLALNGFGPAQTFYTGMTLQIPQNANPFPAQRV
                     LRAHPATYIVIQPYESMGGIACQFGDVDPIAVADANQLAVDSPLFVGQQLHIP"
     gene            8728..10353
                     /locus_tag="PL000_08255"
     CDS             8728..10353
                     /locus_tag="PL000_08255"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88930.1"
                     /translation="MLKEFFTQRIKRGLVRRLNNIKVASAARKVARMAPHPNGAPIVF
                     FKASTGVDDLSWNSGFHLLTTWALRLQGIPVAYFACNSGLSKCVLGTNRDNPHKEMPC
                     KSCVMQSKALYKNVETFERSNVSTSSVNWFGFQRHLELENALRDLNIDQLSHFTFQEL
                     PLGPLCLPGLRWVLRIHHLSDDENTRYLLREYILSAWNVAQKFSDFLDQTQPRAVVVF
                     NGQFFPEATARYIAQKRGLRVITHEVGLQPATAYFTEGEATAYPIHIPAEFELSDAQN
                     AKLDAYLARRFQGDFTMAGVKFWADMQGLDESFLKKAAQFKQIVPVFTNVIFDTSQPH
                     ANTVFEDMFDWLDMVLEIARENRDTLFVIRAHPDETRVRKSSRETVEGWASSRKVEEE
                     ANIVFVSPREALSSYELIQKSKFVMIYNSTIGLEASIMGAAVLCAGKARFTQYPTVFF
                     PQTVDEVRIKTKEFLSADVIDVPSEFKRNARKFLYYQLYRTSLPFGDFLEPSVRTTQT
                     RLRSFDLNDLKRSESVQVILDGLLNGGDFLLKE"
     gene            complement(10429..10878)
                     /locus_tag="PL000_08260"
     CDS             complement(10429..10878)
                     /locus_tag="PL000_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNC88931.1"
                     /translation="MIIDRIENSLPYYTLHPKFKAAFEYLSQINIHSIPVGRHEVDGD
                     SIYALVQEYNTKLKEDGKWEAHRRYIDLQYVVQGAEGMGYANIHHLNQGEYDASKDFL
                     PLFGEGDQVSLKSGCFVLLFPEDAHMPGMAIGEPAFVRKIVLKIAVE"
     gene            complement(10905..11411)
                     /locus_tag="PL000_08265"
     CDS             complement(10905..11411)
                     /locus_tag="PL000_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88932.1"
                     /translation="MEHPTLYEKGVLSWKGMVWLLWKHEAYRNLFYYRIKKDYRIFSM
                     IILELAKLIYKPRNTLFIRADYIGEGLFIQHGYATGISAHSIGKNCWINQHVVIGYSD
                     TGKAPTLGDNVRIAMGAKVFGEISIGDNSIVGANAVVAKNVPPNCTVVGVPARIIKRE
                     GKRVDESL"
     gene            complement(11789..12394)
                     /locus_tag="PL000_08270"
     CDS             complement(11789..12394)
                     /locus_tag="PL000_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC88933.1"
                     /translation="MKLTIFNTPIISPLLRFLSNSTMRLAGWRVEGKLPDLPKYIIIG
                     APHTSNWDFLLFLGVIFRLKADVRYMGKAELFRSPFGWFFYWCGGIPVDRKKSTGLVE
                     QMVEACSKSEKFILTIAPEGTRHYVNEWKMGFYHIAKSAGIPIVMAKVDGKNKAMRVG
                     EIFYLTDDMDADLKAIKGLFEGMTGVNPDKKKKKKYITLEK"
     gene            12450..13298
                     /locus_tag="PL000_08275"
     CDS             12450..13298
                     /locus_tag="PL000_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC88934.1"
                     /translation="MALKSSLRSIIYQTEKIAQRLRFASQPASLPVLLGISFPKSGTH
                     LLDQILLGFSNIAPYAKRVHSFYAEYEGESGKKREPEKALAWLDSLRPADVASAHLFA
                     RPEAITRVISPKFAPYFIFRDPRDVVVSHVFYVTDMETRHVHHDFYQSLPDFNARLKA
                     SILGRPDSGIEFPNIADRFAPYLGWLDQKEVLTIHFEDLIHNREATLTSIMDHLLSRV
                     SLPVTRQLILDSLETSINPKKSPTFRSGKTGEWKKHFTDEHKQIFKEVAGDLLVKLGY
                     EKDGNW"
     gene            13340..14272
                     /locus_tag="PL000_08280"
     CDS             13340..14272
                     /locus_tag="PL000_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88935.1"
                     /translation="MISIIIRAYNEEKYIGRLLEGIKQQTIQDVDVILVDSGSTDSTV
                     AIAESYGTRIVRIRPEEFTFGRSLNFGVKEAKREFIVMASAHVYPVYPDWLETLLRPF
                     QDEKVALTYGKQRGYEGSKYSEHQIFHQWYPDDSNADQTTAFCNNANAAIRKSLWLQH
                     PYDEMLTGLEDLEWAKWAKGQGYKIAYAAEAEIIHIHNETPRGVYNRYRREAMALRKI
                     YPEAHFNFYDFLRLTTTNILSDLWHAARERVLLKNISSIFWFRYMQFHGTRMGHRETS
                     LVTPQLRETFYYARERKMKDERKRDVEPIRYNSE"
     assembly_gap    14296..14395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14427..15197
                     /locus_tag="PL000_08285"
     CDS             14427..15197
                     /locus_tag="PL000_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC88936.1"
                     /translation="MKSKTIVLIHGMFMTSLCWEKWIPYYEAKGYRVFAPAWPGREKS
                     VEELRKAHPDSTLAKLKLNHIVDHMETFIKGLDEKPAIIGHSMGGLVVQLLLQRDLAV
                     AGIAIDPAPPAGVFTTEFSFLKANFPAINPFLLNQPVQMSFEHFQYAFVNTLPLDEQR
                     AAYDRYVVPESRGVPTSSLGAAGKIDFSRLHRPLLITAGEKDHIIPASLNRSNYGKYK
                     GPAVTNFKEFAGRDHFLIGSRGWEEVADYCLEWLGKIE"
     gene            15340..15804
                     /locus_tag="PL000_08290"
     CDS             15340..15804
                     /locus_tag="PL000_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88937.1"
                     /translation="MSKLIPMTQTEFDAFLEQVVPDYAADNVKAGYWSEEEALEKSRS
                     EFMRLLPQGLQSENHYLYTLYDEDQAVGRIWMRANFDRPTKSGFVFELWVDEQFRGKG
                     YGKQAMLLLEEKAREMGLKSIGLHVFGYNTVARGLYEKLGYEVSSLNMSKNL"
     gene            15813..16640
                     /locus_tag="PL000_08295"
     CDS             15813..16640
                     /locus_tag="PL000_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC88938.1"
                     /translation="MPSFSFKPASEFSIPQLADLLTRGFEGYFVPINITPPVLLAMMR
                     RDGIDLTESRVLLKDGGEVGISLIARRGWTSRLAAMGIVSSARSGGAGTWAMQNLIQE
                     AKQRGEKEMLLEVIEQNTAGVKLYEKVGFQKIRRLVGYKLENPKIESGEELEEIDIRE
                     LARMVTYHGLNDLPWQLSGTTVMHFTPPSRAFRLNDAYCLISNPEMNDIAIQSVLVKA
                     RSRGAGLSAVLMRSLFAKFPNKVWHVSPIYPEEMGFIFEHVGMTREDISQWQMSLTL"
     gene            16676..17338
                     /locus_tag="PL000_08300"
     CDS             16676..17338
                     /locus_tag="PL000_08300"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNC88939.1"
                     /translation="MKLAALVPMRHHSQRVPGKNYRPLAGKPLFQHIVETLLAVPEIE
                     TVMVDTDSEPVMEGVRRLFPTVKLIQRPEHLRADDMPMNEILLHDTAQVQADFYLQTH
                     STNPLLKPQTVSRAIQSLFTNYPKNDSLFSVTRLQTRLYFQDGRAINHNPLELIQTQD
                     LPPVYEENSCLYIFTRENLERKRHRIGDTPLMFEIDADEAWDIDEELDFEITDFLMRK
                     RM"
     gene            17446..19458
                     /locus_tag="PL000_08305"
     CDS             17446..19458
                     /locus_tag="PL000_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88940.1"
                     /translation="MQTKNEKSNPFWLSALIIFVVSAIAYLPLARKFGYLNDDWYLMY
                     DMWVKDPSFFHVIFSGDRPGRALAMIPLFSLFGFEPLYYHLSAFLFRFLSGVSLYWTF
                     RMLWPWKRFAALAAALLFAVYPGFLSQTNAIDYQSHILGLFLAALSVALTIKSLLTVE
                     RFPRFFFMAASILAGWGYLSQMEYFIGMEAFRFGCIFILMWRTQGASYLQKAGNWFIA
                     SLPFLLVAGGFLIWRLFFFESERKATDIGLQISQLTSSPLTLLWWLNYLVQDMVSVTL
                     VAWGLPVYVLAFPMRLRDVMSAFGWAGLAVALMVLGMNRVDKSKSEAEAGPSLSREAL
                     ILSLISIVGGLLPVILVNRHVTLPDYSRYTLIASVGAALLLVTIIEKISARVALLSFF
                     VAIAVLTHYGNAVRAVDEAQATRDFWWQVAWRVPQFQPGVTLIASYPGSPLSEDYFIW
                     GPANLIYYPEKQDANPVEIKLPAAVLTDDVVLEIATNGGVETPLRRGNYLERDFGNVL
                     VMIQSRSNGCVRMINGDVPELSPLDQHRLLLIAPFSRLENVKTEGEFPTPPAVVFGSE
                     PERGWCYFYQKADLARQRGDWEQIPVLLKEALDAGHYPEDALEWMPFLQASAVLGDVE
                     QVRSTAKLVTVDKFLRLQVCNIMTDFMEKETLNDEVKSVIEHTICK"
     gene            19558..21090
                     /locus_tag="PL000_08310"
     CDS             19558..21090
                     /locus_tag="PL000_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88941.1"
                     /translation="MKKFFSSFFNAPWYPIVISAYPVLALLSSNAGQVQSNTGIRPLL
                     VSVAFGGLLFFIVWVILRRQVHRAAFLTTLLLALFFTYGHAYIAVDEKWPDAGYAQWL
                     AVVWAILLVLAVFWATRPKWTFVSAAANLNTVALALVIMAAAQIDFSNGMGKVHRVAL
                     EKAPIQEDLTLPENPPDVYFFLLDSYGRSDLLYAAYGFDNSDFENQLVDRGFYIASCS
                     QSNYVRTEISLGSSLNMAYLQGLDETFTPDSTARRVLWDALKHNAVRYNFETLGYETI
                     NFASGFAWLEITDVDHFMSPPPASSGMNEFEGLFLRTTLARYAQDLGWVDPDYLMGVS
                     FRDRFNYIFNSMDEITAMPQPTFAYMHLISPHPPFVFDPEGNPTYPPDFWNDQRMYPA
                     DMYQKGYVNQLQYLNKNMLEAVDTILANSDTPPIIIIQSDHGPWLQPKDKRMRNFTAI
                     LMPGHEDVLYPTITPVNIFRVVFNAYFGGKYDILEDVSYFSPVPKLYDFSEIKNPCAN
                     GN"
     gene            21094..22044
                     /locus_tag="PL000_08315"
     CDS             21094..22044
                     /locus_tag="PL000_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC88942.1"
                     /translation="MKKTVLLTAPYMLPFLERFRPEFEKYDLDLIVPDVEERMEEADL
                     LKYAGQFDGAVCGDDRYTARVIEACSPRLKVISKWGTGVDSIDAEACSRFNVILSRTP
                     NAFTTPVADTVLGYMLAFARRGPWMDREMKNGKWEKIPGKALSELTLGIIGIGNIGKA
                     VTRRAKAFGMKVLGTDIVDVDHVFVNESGIEMTDLHSLLSNSDFVSVNCDLNPTSHHL
                     INAKTLSLMKNTAVLINTARGPIVEEPALIAALRSGQVGGAALDVFEYEPLPKDSPLL
                     KMDNVMLAPHNSNSSPTAWERIHWNTIKNLVTGLGLEYKG"
     gene            22052..>22314
                     /locus_tag="PL000_08320"
     CDS             22052..>22314
                     /locus_tag="PL000_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88943.1"
                     /translation="MSKLRSALPYLFIFLLAALVLDLGNPLFDKPARDGGFFLYAGSQ
                     IISGKIPYVDFWDSKGPAIFYINALGLFLGQGSRWGVWGVEFV"
     assembly_gap    22315..22414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22415..23731
                     /locus_tag="PL000_08325"
     CDS             <22415..23731
                     /locus_tag="PL000_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88944.1"
                     /translation="GLRIVLGYGNYTEEYALLFNALAVYLFFVVDENKGWKYFGIGLL
                     FGISFSFRANNIGGLFAVLIALALAGFVGNLAKTPNPNHRDTESRRFNGFFPGVSMSP
                     WSKSFFIRLLFVLAGFAAPLLLWVLFFQIHGAAWEMIFASIIFNFSYASAKSREWLDI
                     FGGFGRYGMLWVGWVAALGYVVNGYHVLRKALHRNPLTLVDWFLFTWFPFEILLSNLS
                     GRNFSHYYISWALAVAVYGAALFTSGTGFSLPKSLTALTTGVRTSFLYDGMVLFFIVG
                     LLLFSTPSIKRYGETVSRSFSGQSLEYSDPLVDAIRANTQQDDLVLTWYPETTLNFMA
                     GRASPVKYVYYPLFLEGTLPPGAETSYIEGLTSRRPEMIVDCSRAVDAIPSLDPAARE
                     DQFSKPGVKKKMYFQPGMEEIFNFVAENYHVETSVDGCLLFRLNLE"
     gene            23735..24496
                     /locus_tag="PL000_08330"
     CDS             23735..24496
                     /locus_tag="PL000_08330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC88945.1"
                     /translation="MDTQKFVLITGAAGGIGRATVNLFFEKGWHVVGIDRSDFGEGFP
                     ANGLFVRSDISRPEDMQAIFEKVSAVTDSLDALVNNAAMQVAKPLVETTVEEWDAVMN
                     ANLRSVFLGVKLAHPLLKARGGAAVVNVSSVHAIQTSANIAAYAASKGGLLALTRAMA
                     IEFAPDNIRANAILPGAVDTPMLRAGLGRGHVGQGDVQDRLDNLARKTVNGRVGTPEE
                     IAHAIYFLADNQQSSFMTGQALVVDGGATARLSTE"
     gene            24581..24985
                     /locus_tag="PL000_08335"
     CDS             24581..24985
                     /locus_tag="PL000_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88946.1"
                     /translation="MKRRTRKTKFLTDRAYVPASRKTLVRILFFINAALWLGYVVYIY
                     YDMAVVNKNLTSADIVSLFVFANSVMMFVSGWQLAKNESWAYYLAMVVIGINLLLTLL
                     NILDLFFLGIFLLDLFILLSLIPIRKHYLSKP"
     gene            24982..25317
                     /locus_tag="PL000_08340"
     CDS             24982..25317
                     /locus_tag="PL000_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88947.1"
                     /translation="MKNIKTVGSLFFVTAFVLAVFGGASFSRAHANPIYNLYAVLMFV
                     DAAVMLVCGLYINRHTQVVYWFAVAALTLNIVLTIFDQIGLVDILFAALNFVTLMALV
                     SSRKEFSHQ"
     gene            25314..26129
                     /locus_tag="PL000_08345"
     CDS             25314..26129
                     /locus_tag="PL000_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNC88948.1"
                     /translation="MKQTIKSLFPESTFNVVRDAYDAVRRIPQLPDAYLHPWRRESIR
                     RLAALKDIHKGQRAFIIGNGPSLKQTDLTKLKNEFTFGMNRIYLLFPELGFHTTYFCS
                     INDLVIEQFSDDILALPMPKFLAWRSHRHFSKNLQLANIPTFLYTTYTGPRFTPDVRR
                     RVWEGATVTNFALQLAFHMGFEKVILIGVDHNFASKGDANKTVVSEGDDPNHFSPNYF
                     AKGFKWQLPDLDTSEIGYTFAREAYRKAGREVLDATVGGKLTIFPKVDYNSLF"
     assembly_gap    26256..26355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26356..27510
                     /locus_tag="PL000_08350"
     CDS             <26356..27510
                     /locus_tag="PL000_08350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC88949.1"
                     /translation="LFWLAAGIALFDLMRRAVSPWAAVVAIAFYFVLPFSVQASRSFQ
                     PDPLMTAAFVAGIYFLYRWSEEQTWKWAILTGVFFGFATFVKIVIAFFVGAAAISLVL
                     FTLKKDFWKSKQVWVMAAIMVVPAFLFYILLNQGRSTEYFFAWTVTLIKLITSADFYS
                     KWLAFVGSIFGLTMIFLSLAGALIATPRLRWLLVSLWVGYVLYGLTLPFQMYTHSYYH
                     IQLIPIIALGLASALNPLIESVAGQGQVGRVGVIALIVVVIGYQSWVARSVLIAEDFH
                     HEPAFWKQVGDAIPANTDVIALTQDYGYRLMLFGWRKVSLWPLSTGLSEARGGNTDVA
                     GNFADLTAGKEYFLVTAQGQFDKQPELKKILSQYPIAAQGEGYVLFDLRK"
     gene            27507..28436
                     /locus_tag="PL000_08355"
     CDS             27507..28436
                     /locus_tag="PL000_08355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC88950.1"
                     /translation="MTLVSIITPSYNQAAYLEQTLLSVLNQDHAPIEYIVVDGASKDA
                     SVEIIKKYSDRLAYWVSEKDGGQAEAINKGFARATGEIIAWINSDDYYLPGAVSAAVK
                     IFESNPDVVLVYGNMLAVDEHGKTFNTLTYKQLTLEDLLCFQIIGQPAVFMRRSALQK
                     TDGLDSTFHFLLDHFLWIQLAQHGKILHADQTWAAARYHAEAKNRAKAAEFGREAFRI
                     LEAVAQDKNLAPVLSKVERRSRASAHRVNARYLLDGGQPAAALSAWVRAFFIHPPTAL
                     ARMNLFVSAILNLFGLGKLRSMVLRMREARHRE"
     gene            complement(28654..29121)
                     /locus_tag="PL000_08360"
     CDS             complement(28654..29121)
                     /locus_tag="PL000_08360"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC88951.1"
                     /translation="MPYLIDGHNLIPKVGLRLDSSDDEVKLTRLLQNFARLKRETVEV
                     YFDGAPPGQAGTRKLSGITAHFVRAGQTADSAIRARLKNMGKTAKQWTVVSSDREVQS
                     AAQTARAQFTSSESFSKLVEQVINSAPPGATDERLSAAEVDEWLEIFQDKSTK"
     gene            complement(29196..29588)
                     /locus_tag="PL000_08365"
     CDS             complement(29196..29588)
                     /locus_tag="PL000_08365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC88952.1"
                     /translation="MEMLIDFPGGLRVDAHFRGHTIATDQPPADSAPMPFEVFLASIG
                     TCAGIYVLGFCRQRNLPTDGIHIIQRNHANPMNGMVSEIDLEIQVPPSFPAQYYDALV
                     RSAELCKVKKTLENPPTFNVMTKVLEPA"
     gene            29746..30108
                     /locus_tag="PL000_08370"
     CDS             29746..30108
                     /locus_tag="PL000_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88953.1"
                     /translation="MTEKKNTNITVSPQSGMIHNLVVRTKLVLRLLGDKRVSSWAKLI
                     PVGSMIYLLSPIDAIMGVPGLDALDDAAIVGLGYYFFIELCPPDVVQEHLQALEGTNE
                     PPQAREDVIDGEATEVKE"
     gene            30109..31347
                     /locus_tag="PL000_08375"
     CDS             30109..31347
                     /locus_tag="PL000_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNC88954.1"
                     /translation="MRRFLILLTLPILACGGLAESVVATITPQAAPTIAAPPQTESPP
                     TVDSATEPPVISPTEVTAANVNTFPNTDNYQWTLIASGLSRPVDIQSAHDGTGRLFIV
                     EKYGVIRVYENGQLLDQPFLDISDRVGDESNEQGLLGLAFVPNNSGVLFVNYTDNNGN
                     TVISRVLWDQVANPLSEKDLLHVEQPYPNHNGGALAIGPDGYLYIALGDGGLAGDPHK
                     NGQNTNSLLGKILRIDISDLNNPNNYSIPADNPFGNEVWAYGLRNPWRISFDRVTGDL
                     WIGDVGQGDWEEIDYLPAGSAGGANFGWSVMEGSYGYDGVAQPGMLLPAAEYSHNEGG
                     CSVTGGYVYRGAMTEWNGIYLYGDYCTGKIWGLILSNGQWQSQVLFEAGETITTFGQD
                     EFGEIYFASDSGNVYRLTKK"
     gene            31459..31677
                     /locus_tag="PL000_08380"
     CDS             31459..31677
                     /locus_tag="PL000_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88955.1"
                     /translation="MAEKIWQVEKIKYCEHVGHEVAIENEVVYPAEHLPEQLPRIVAH
                     RCSNAIECNLIDKATCALCGTNPDLDPV"
     gene            complement(31786..32520)
                     /locus_tag="PL000_08385"
     CDS             complement(31786..32520)
                     /locus_tag="PL000_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC88956.1"
                     /translation="MSETKTTNLIEIRALRVQRNQRDALHVASLDIPRGETLAIVGPN
                     GSGKSTLLLTLAHLLRPASGDVKYGGKSLREWNDLEYRRKIAFVFQDPLLMDMSVEEN
                     VALGLKFRGMDKPESQMRSEKWMRSMGVDSLSKRRAGQLSGGEAQRVSLARAFVLDPE
                     LLLMDEPFSAVDPQTRAQLLQDLSTLLSHDHRTTIIVTHNLKEAAQMGDRVAVIINGE
                     LKQVGTPQEVKNSPADESVERFLKEL"
     gene            complement(32517..33161)
                     /locus_tag="PL000_08390"
     CDS             complement(32517..33161)
                     /locus_tag="PL000_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC88957.1"
                     /translation="MSEIFEITALSLQVSTIATFVSLLIGLPLGTWLALGNFRGRAFI
                     LSIVNTGMALPPVVVGLVVAMTLWRSGPLGSLRLIYTPWAIVIAQTIISAPVVIGLTA
                     AALAALDPRLQQQLLGLGASRLQMIWHLWREARLPLLAALMAGFGSVISEVGASMMVG
                     GNIRGQTRVLTTAIVLETSKGEFERALALSALLLIITYLINLALTWVQQKGQRR"
     gene            complement(33248..>34205)
                     /locus_tag="PL000_08395"
     CDS             complement(33248..>34205)
                     /locus_tag="PL000_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC88958.1"
                     /translation="KMKQTRNILLTLGLILAIALSACGSPAPTAAPATEAPVATEAPA
                     TEAPTEAPTEAPAAAPANPNLILATTTSTQDSGLLDVLVPMFEEQTGYTVQTVAVGTG
                     EALKMGEEGNADVLLVHAPSSEVTFMDGGFGKDRMLVMHNDYIIVGPAADPAGIKGLG
                     PKDAFVAIYNAGAPFVSRGDDSGTHKKEVSFWTKAELDPRTEKPAWFIETGQGMGASL
                     TVASEKGAYILTDRATYLSNKANLQLEILLEGNNALLNVYHVITVNPDKWTAVNYDGA
                     MAFAKFITDPATQAVIAEFGKEKFGQPLFYPDADKTDADLGL"
BASE COUNT         7849 a         8435 c         8576 g         9045 t
ORIGIN      
        1 tccgccgcgc cttccgccct tacggcaaaa ccgcccaagc catctggcgt tggaagcggt
       61 tttcttttaa cgatgccccg cccatctttg ccaactcaaa accgaagagc ggttcgcatt
      121 tgcttttgca agtgatgaat gggttcaccc agatcatgcc gtacaaatat gtggatgccg
      181 accccgtgcg gacgatcaca tatgatggaa gacgaaagaa taaagaagaa atccttgcag
      241 acttgaagca cactcccaaa ggagtgatcg gctggggcta tgtggatgcg acggaagaaa
      301 acgcttcttt cttaactgcg gctgggcgcg tgaactattt tatttatcgc gacccgagag
      361 atatgctcgt ctcacaagtg ttcttcgcga ccgatatgca cgaagagcac ggcatgcacg
      421 aatactacaa gtccctgcct gattttgccg cgcgtttgaa tgttgccatc acaggcattg
      481 accgtgacgg gctgaagatg gtcagcgtca aacaacgcta cgaaggcgtc ttccaatggc
      541 tcgaacagca gaataccatg tgcatccgct ttgaagacct catcaacaac cgcgatgcaa
      601 ccttgttgaa gatgcttgat gaagtggaaa agacgggcta caagattccc actccacgtg
      661 aaaaggcatt gtccattttg gtggaagcga ttcagccaaa aaagagtcac accttccgct
      721 ctgggaagac aggcggctgg aaacaacact tcactgaaga acacaaaaaa ctattcaagg
      781 aagtgacagg gaatctgttg gtgcggctgg ggtacgaaac taccaatgac tggtaaaacg
      841 aaaatctgag gcagaacgcc tcagattttt tatttcaact caaaatcgct tctccagtaa
      901 actttcaaat ggtgtaggct agaacatcgc agataacaga attttcaacc cgccgccaat
      961 ggcacccaaa acgatgctcg aaataattaa tgggggcaaa tggttatatt gctttggaat
     1021 gatgctgatg ttgatatacc acggtaaatt caagaaaacg gcaagcacag acccaagcaa
     1081 ataagcaggg aagggggaat cgacaactcc gatcataaat ccacaggtga accaggctaa
     1141 catgctttgg atatatatcc aaaaccccaa tttttgcctg gagccgacaa ggatgaccca
     1201 ttccaatacc cccaatacgc ttcctatcaa aacggcttta attaccattc catcctctat
     1261 aagtacgact ttagcacact caaatgcatc acacctgcag agacatcttc atggttcaca
     1321 cctgtcatca actcaaaata tttaaagcag gcagggttgt tcatggcttc ctgcatggcg
     1381 gcgtccacac tttcctggct atcccaatac accacatccg cccattgacc atccgcgcct
     1441 ttgacaaggt cacgtttgat aaatcccttt tgtgatttta gaaacccttc ttgcatggca
     1501 tctgatgcgg cgatcaatgt agcttccgat gcgccgtctg ccagccgaaa tgatgcgagt
     1561 tcaattactt gatttgacat ataaatctcc ttgattgaat ttccccgcat tttacaaaag
     1621 aggtgtgaca accctatgtc aacaagtttg ccagtttttt cgccgtctcg cgaagttcct
     1681 ttcgcaggtc tttcggcgca agcacttcag cctccgcgcc ccagccaaga atccacgacg
     1741 tgatctccga aacatcatgg acgtgatagg tcatcactgc atccttttca cgcacaaatc
     1801 cataatgttg gcgctcgcgc acccaacgca gaatccgcgc atcgaagcgg atgcgcactt
     1861 ccaccatccg cgctgccgcc gctttgctct tgcgccgttt tgaaaaatgc tgatcgagca
     1921 gggtcaactt ctccacacgc gaaagacgaa attcacgaac atctttccgc agacggcaat
     1981 aggcttcaaa ataccaaaca ccatctgagt aatacagttg atgcggttcc acatcacgct
     2041 cactgatttc atctttgcgg tagccgtgat aacgaatatg catcacctgc ttttcctgaa
     2101 tcgctttttg gatgaggagc aactgcgagt cgtcgaggtt gaatttctga ctcggcgtaa
     2161 tgaacccgat gatgccgctc aacgcctccg acttaacccg aacctgctcg ggtaacgcga
     2221 ccttgatctt ggctgaggca gagtctgcgg cttgggtgag cgaaccagcc gcctgttgag
     2281 tcaagaggcg cagaccgaga gtcagcgcaa cggcttcgtt ttcgttgaac atcagcggcg
     2341 gcagataaaa tccttccacc agttcaaatc cctcacctgg caaggccgcg atcggaatgc
     2401 ccatctgatt caatgcagac atatcgcgat agatcgtgcg cttgctgatc tcgaactgac
     2461 gcgccaaatc tactgctcgc aggcggcgtt tatgttggag ttcgagcaag atggcaaaga
     2521 gacggtcaat gcgattcatg gatttcccta tgtacgggag tatacccgag gcatgggaac
     2581 tctttgccgt aatgagccaa tatgtaacag tgacctactt ctcgaagcgg atttcatcca
     2641 gataaaatgt tgctccaccc ggacattttt cccagcttgt tgaccataca aaaccgccaa
     2701 tgatgtagga taagtctgcg ccctcaagcg gaatttcata ttcctgccat tcggttgtca
     2761 acctttcggt gataccgcgt ggatacacaa gactatcgcc ataatctcca gtgatgccgc
     2821 caactttgaa ttccaatgtg caggtagatt cagaccttgc ccagaatttc aaagttcgat
     2881 atgcggagag atcgaaacct gttccagccc agatctgttc cttgccccag ttattggctg
     2941 gttgctgcca gtacactccc gcccatttgc aaggtggttt gtatgcacag gcatatggcc
     3001 cctgaccttt aggagtgtat atcacctgaa tggaagtatc gcccgattgc ggattttccg
     3061 tgctggctcc atcaatttga atgtcaccag tatcgcccat ataaccgctt ggactgaaat
     3121 ggttgtaagg ggatgctccg tcgttgtaaa tgtagaaaat atcatcagag atggggagtt
     3181 ctgtttctac aggaatttct gtagctgtcg cagttgggaa ttccacgacg ggttctccac
     3241 ccatgaagct gaccgccgtt tgcttgggag tccgatctga gaaaaagatc ccccaatgtg
     3301 gctccacaga tagatttgtc ttccagggtt gatcaaatgc ttcaaagtac acgaatttca
     3361 catctgtata ttccaggcct tgatagtatt gatcttgtgc tgtttctgac aggttatttt
     3421 cagcatctcc ttcggtgggc agtcccactt ccttgaagag aataaaacgg tctgttttgc
     3481 tgctcagatc tttgtaggcg ccctcggtcc actggacagc gttgacaggg tcaatctggc
     3541 tgtgaaaata tggatgcgca tttggaaaga cccattcacc tatttccaac agtttttcat
     3601 cgtaatagtc atcaagttgt tcggctgttg tgacgggttt acctgttgtc ttgcgaagtt
     3661 cttcaatggt tgaagacaga acagtcatgt catatctcac gtcgaggcct tcattgccca
     3721 cacaaaaccc aagcacaatg ggattgctcg aagcagcctg tgcatttgac atttcttccg
     3781 cgctggtggg gtcccaaata cccataatga cccctttgaa accagtttcc tttgccagag
     3841 tggggaagtc acgccccatc accccctttg aatcgtatgt gactaatcct gtaaaacctg
     3901 cattgtgcaa aacctcaagg tcggcctgaa tatcctccgc acttgcctga atccccttgt
     3961 tggggtttgc gttgggcggg ctataggcga tccagatgat attgcttact tttttttccc
     4021 atccgcttgg gagtgcgggg gtggatggac tggtttgtgt ggcagtgggt gggttggcac
     4081 aacctgatat cagcaggaca aaccagatga gggtaagtgt gtgcagtaca tatttagtgg
     4141 acatttattt gctcccgcaa tcgctggttg ctgggtgttc tacgatcgcc ttgtcgtaat
     4201 aaatatttcg tatttcaatg acaagggttt tgggattttc agggacaatg cttgaatcag
     4261 ggttggtgat cgaggtgatg ttgttgtcgc cccaatcaat tgtccagccg aagaccccta
     4321 caacatgttg aaatgaatcg gggggaaatc ctgatatgtc atattcgaaa ctttgccatt
     4381 cctgggtaag ggttggttcg cacaataaca cggatgatct caccgaatct ggatagggag
     4441 ctacaacccg ctcctttgtt tgctcatccc atgtccatgt gacccctcca atgacaaatt
     4501 taacatccac aaggccttga acacttctgg cttcccagcg tatggtttta taagcgctca
     4561 agtcaaagcc gttattagga tctgttccac ctgggcctgc cgagtcaatg aacatgatcc
     4621 ctgcaaatct ggcggggttg gggtttattt ccttgtttat atatttatag tcccattcat
     4681 gcggaccatt tccattggtg gagtaggtga agataattgg attggcggta tctccaatgc
     4741 cgctgatatc aatatccccg cgatctccca tgtgttgagt tggcttgtaa aaccgattca
     4801 ccaaaaagcg atctgcgttt tcagggggca ttttaccgac caacccatcc tgtacgttcg
     4861 ctgaacttgc atcaggtgca tcggcagatt gtgtggctgt ttctggtgca tctttgatga
     4921 ataccggaat gatggcgatc acggcggcgc tgacggttgc aacagcagcg atcaatgcca
     4981 cgatgatttg cgcccattct tttgtggtct ttggcgagtt ttttgctgtt gaccttttcc
     5041 tgcccaattt ttacctcctt gaaactgtaa ggatgacagg gtggaaaacg aatttattgt
     5101 accttttggt actatcagta taggtcaaat aaacatgaaa aacaacaata ttttctgcct
     5161 gctgtgattt gaagaacaaa acccctaata aagtgtgttc aagtccatac tggggtgggt
     5221 tgaaaacggg tgtgaggtac cgtataaccc attagatgag accgaaattg gatttggcag
     5281 ggaattctgc tttggtggaa tctccgcctc gcgtacggat ctggatcagt gccttgcgtt
     5341 atttgctggg aaaagcactg acgattttcg ccaccatctt tgcgggagtc tttctcacac
     5401 ttttacttgc aaaccagcct tccccgcgcg ggcttgaccc tgcacggtct ccatttgaga
     5461 tcagtttgga atcccagatc gcgcgcgtga tccgtattag cattgcaaat gggacgatcc
     5521 caaggttgtc agatgggtac ccagatcagc atcagttgga tgcgcttgag aaaaaactgc
     5581 gtgacgatgc tggattggaa cttccatttt ttccgcgtta tttatggtgg acattgaacg
     5641 cgcttcagtt caactgggga gagctgactg taacctcgac cgaaacaaca ggatttagtc
     5701 tggcagaccc cgttggaaaa cgggatcttg tgttggaata tttgcccaac accctgcttt
     5761 tgatcggaac cgcatttttg ctgatatttc tgatcggcat gccattgtca ttgtatctgg
     5821 cgcgaaacta tggcggctgg tttgacaggc tgttgacatt cctttcgccg atctcttcca
     5881 ttccgagttg ggttgtggcc atgctgctga tcgctttgtt tgcggtgaaa ctgcgctggt
     5941 ttccagtcgg cgggaaattt gatttcgcca ctccgcaaga cccgcttgaa tatatattga
     6001 cgcttttcag gcacatggtg ttgcctgttt ctgccattgt tttcagcatg ctgtttcagg
     6061 tggtttatgc ctggcgtact ttttttatcc tctattctga agaggattat gttgaccttg
     6121 cacgggcaaa aggacttccg cacaagatat tggaacggca atacatcctc aaaccttccc
     6181 tgccatacgt gatgaccagt ttcgccacga tcgtcattgg cttctggcag ttgaccgtgg
     6241 ctttggaggc ggtttttcaa tggcctggaa tcgggtggtt gtatattcgt gaggcattgc
     6301 ctaatttctg gggcgaaagc atggatcccg gacaattgat gctggtgatc gagatcgtgg
     6361 tcatttttgc gtatttgctt ggcgcggtcg tgttttttct ggatctggct tatgcccttg
     6421 ttgatccacg aatccatttg ctgccatcgc gtaatacgac gcaggggcgg gtgaaaacca
     6481 gaatgcaggg aaccgactgg ttggcccgat tcaatgcctg gatgaaagag aaaacttcga
     6541 gaaaaccgaa gcaggccaca ggtccagtgc gaccgttgaa attcatttgg gaggcgtttg
     6601 cagattcgct ccatgatttt gacgcacgga gcagactctt cttccatgaa cttcgcagat
     6661 atccatctgc catgtttggg ttgaccgtca tctccattat gctggtggga tccatttatg
     6721 ctgtgatcgg gttgccttat gaaaaatatg gcatggagta caacacaaat cgggtgacgg
     6781 ggcgcagtta cacgccacgg ctggctgttc ccgcttggac gaatttgttc agttcaatgc
     6841 cgcggctttc aacgcttgtc attaacgaaa agagtccacg agctcaggta tcgctgcggt
     6901 cattggaaaa tggctgggtc gaaaaaacaa taacattcac ttttgactat gcctaccgcg
     6961 aaacgccgag cgatattttt ttatatcttg agccgaagta tgttgaaaag tttcctttca
     7021 tctcgttgac gtggatcacg ccagacggca gggatcttga tttggttgca aagtccatca
     7081 atgcggttac gaattatgat tttgagacag gtattcgcgt tgcaaaactg attcgccaaa
     7141 atcccgaatg ggaaaagtgg tttgttcggt cggggcaata cccaacgcct gccattaacc
     7201 ttctctttgc aacaccacac gaaccgcaat tgcacttaca gagcgggcaa tatcaactgg
     7261 ttcttaaaag cctgctcttt gaagatgaaa gcgacctgac tgcagaactt gttttgcttg
     7321 ggcaggtata tggcgcggca ggcacagatt actggcgccg cgatctggtt gtcccgttgt
     7381 tgtgggggat gccttttgca cttattatcg gtttgcttgg gacacttgtt accattcttg
     7441 ttgcgatgct tttacccgcc attggggtct ggtatggcgg ctggctggat gagttcgttc
     7501 aacgcctcac cgagatcaat atggtgttgc caggattaat gatcgcagtt ctggcttacg
     7561 cattatttgg aatcaatatc tggatcgtgc ttgggcttgt ggtgatgatg aatgctttcg
     7621 gttctcccat caaaaccttt cgttccgcat tgttacaggc cagggaggcg ccctacattg
     7681 aaatggctcg ctcctatggc gcaaccgatt tcaggatcat tacacgttat ctcgtgccgc
     7741 gcattcttcc tgttgtaatt cctcaattgg tctcccaaat tccaggcttt atttttcttg
     7801 aggcaacact gggtttgttc aatatcaaat ccaattatcc atcgtggggg cgaatcattt
     7861 atgacgggct ggcacgcaat gcattgtatg gttcgccctt ttgggttctg gcgccgatct
     7921 ctttgctttt gcttacaggc cttgcgtttg ctttgcttgg cacggcgctc gaacgtgttc
     7981 tcaatccgcg catgctcgag caagtctctc aagcagagga aaaggaaata gcaaaacaac
     8041 gccgcgtttc aaaaaggatg atcgcaacaa tcactgccgc agccttgttg atgtttttta
     8101 tccttgttcc ttccatcaaa accctctctg gaatgttcaa ccgtgcgcca tcaactgcct
     8161 ccgatagaat ggccgtgcct gtggcagtga ccctgccctc agtcaccgct ccttctccga
     8221 ccgaaacgcc tgatcctgtt ccccaggcat cttccacgct gacattttcc ccgacaccca
     8281 ctctaactcc cagcccgacc atccttccga gcctgacaca cacatccacc ccccttgacc
     8341 tgcgcccaaa gacctacact ttgcaggcag gggaattccc ttattgcatt gcacgtcgtt
     8401 tcgatatcga tcccatcgac ctgcttgcct tgaacggctt cggccctgct caaacttttt
     8461 atacaggaat gaccttgcaa attccgcaaa atgcaaatcc ctttcctgca cagcgggtat
     8521 tgcgggcgca tcccgccacc tatattgtta ttcagccata tgaatctatg ggtggaattg
     8581 cctgccagtt tggagatgtg gatccgattg ccgttgcgga tgccaatcaa cttgccgtgg
     8641 attcccccct gtttgtcggt caacaactgc atattccata gtgccgtaac aaatgatttg
     8701 ctgataagat tgggggcgag aaaatccatg ttgaaagaat ttttcaccca acgcatcaag
     8761 cgcggactcg tccgcaggct taacaatatc aaagtcgcat cggcggcacg caaagtggct
     8821 cgtatggcac cacacccaaa tggtgcgccc attgtctttt tcaaagcatc cactggcgtt
     8881 gacgatcttt catggaacag tggtttccat ttgttgacca catgggcgtt gcgcttgcag
     8941 ggaatccctg tggcgtattt tgcttgtaac tcagggctga gtaaatgtgt gctcggcacg
     9001 aaccgcgata acccgcacaa ggaaatgccg tgcaaatctt gcgtgatgca gtcaaaggcg
     9061 ttgtataaaa acgttgaaac gttcgaacgt tcaaacgttt caacttcaag cgtcaattgg
     9121 ttcgggtttc aacgtcacct tgaacttgaa aatgcccttc gtgatctgaa tatcgatcag
     9181 ttatcacatt ttacatttca ggaacttccc ctcggcccgc tctgtctgcc tgggttgcgc
     9241 tgggtcttgc gtattcatca cctgagcgat gacgaaaata cccgttatct tttgcgcgag
     9301 tacatcctct cggcgtggaa tgtggcgcag aagttttctg actttctcga tcaaacccaa
     9361 cctcgggctg tggtggtttt caatgggcag ttcttccccg aagcaacggc tcgatacatc
     9421 gcccaaaaac gcggactacg cgtcattacg catgaagttg ggttacagcc tgccaccgcc
     9481 tatttcaccg aaggggaagc caccgcctac cccattcaca tccccgctga atttgaattg
     9541 agtgatgccc aaaatgccaa actggatgcc tatcttgcca gacgctttca aggcgatttt
     9601 acgatggcag gtgttaaatt ctgggcagat atgcagggac ttgacgaatc ctttctgaaa
     9661 aaggcggctc agtttaagca gatcgtccct gtctttacca atgtcatctt tgataccagc
     9721 cagccccatg ccaacaccgt ttttgaagac atgttcgact ggcttgatat ggttttggag
     9781 atcgcccgcg agaaccgaga taccttgttt gtcatccgcg cgcaccccga cgaaacgcgg
     9841 gtgagaaaat ccagccgcga aactgttgag ggttgggcaa gcagccgcaa ggtggaggaa
     9901 gaagcgaata ttgtctttgt aagtccgcgt gaggcgttga gttcctacga gctgatccag
     9961 aaatccaaat ttgtgatgat ctacaactcc accatcgggc ttgaggcttc catcatgggg
    10021 gcggcagtat tgtgcgcagg caaggcgcgg tttacgcagt acccgacggt gttttttccg
    10081 cagacggtgg atgaagtaag aataaagacg aaagaatttt tgagcgcaga tgtgattgat
    10141 gtgccatccg aattcaagcg caatgcgcga aagttcctgt attatcagtt gtatcgtacg
    10201 tcgcttccat tcggcgattt tcttgagccc tcggtgcgga cgacgcaaac gcgcttgaga
    10261 tcgtttgatc tgaacgacct taagcgctcc gaatcggtgc aggtgatttt ggatggactg
    10321 ttaaatgggg gagattttct gttgaaagag tagcaactgc caggttttcc ttgaatttgc
    10381 agttgctaca ctatactttt tgattctttg tgtttgcaga atattttatt attccaccgc
    10441 gatcttcaaa acgatctttc tgacaaaggc aggctctcca attgccatgc caggcatgtg
    10501 cgcatcttcg gggaagagta aaacaaaaca accgcttttc aacgacacct ggtcaccttc
    10561 cccaaacaac ggaaggaaat ctttgctggc atcatattcg ccttgattca ggtgatgaat
    10621 gttggcatac cccatgccct cggcgccttg cacaacatat tgcagatcga tgtagcgacg
    10681 atgcgcctcc catttgccat cctctttcag ctttgtgttg tattcctgca caagggcata
    10741 gatcgaatct ccatccactt catgccgccc gacggggatg gaatgaatat tgatctgact
    10801 caggtattca aaagcagctt tgaatttcgg gtgaagcgtg taatatggca gggaattttc
    10861 gattctatca atgatcattt ttatataacc gttggtggat gtgcttataa cgactcgtcc
    10921 actcttttgc cttcacgttt gatgatgcgc gccgggacgc caacaactgt gcaattgggg
    10981 ggaacattct ttgcgacaac agcatttgca cccactatgg agttgtcacc aattgagatt
    11041 tcgccaaaca cttttgcccc catggcgatc cgtacattgt ctcccaaagt gggggctttg
    11101 cctgtgtcac tgtatccgat cacaacgtgt tgattgatcc agcagttttt cccaatgctg
    11161 tgagctgata tgcccgtggc atatccatgc tggataaata acccctcgcc gatgtagtct
    11221 gccctgatga aaagggtgtt ccttggtttg tatatcaatt tggctaattc aaggatgatc
    11281 atgctgaaga tgcgataatc tttcttgatg cggtaataga acaaattgcg ataggcttca
    11341 tgtttccaaa gaagccagac catgcctttc caggataaaa cacctttttc gtacagtgtg
    11401 gggtgttcca tttccatcca ccttgtgata tcggcttcaa tgacatgccg tgatggagag
    11461 gtggtcaaaa gaaaaatgtg gaagatggat ctgaggatat gaaatgcaaa tagtatatcg
    11521 gtggctgttt gaaatagata gctgctctta aatccgttgt ccctgttcat agcatcccta
    11581 tcgcatcaaa gttcttgaga tacatgaact ttgtatgcca gattaaattg ctgtcgaaat
    11641 tctaccatcc ttgcagcgtc acgatcatat gaatatgata ataaatctcc cgcaattatc
    11701 actaatttga tttgaattgt ccgaccagtg atacacaatt ggaaatggcc tgaacttaat
    11761 aattaatcca gcacgaaaga gggaaaaact atttttccaa tgtgatgtat tttttctttt
    11821 tcttcttatc agggttgacg cctgtcatcc cctcaaataa tcccttgatc gctttcaggt
    11881 cggcatccat atcgtctgta agataaaaaa tttctcccac ccgcatggcc ttgttttttc
    11941 catccacttt tgccatcacg atcggaatcc cagccgattt ggcgatgtga taaaaaccca
    12001 tcttccactc gttgacgtaa tgacgagtcc cttcgggggc gatggtcaga atgaactttt
    12061 cagacttgct gcaagcctct accatttgct ccaccaaccc agtggacttt ttccgatcca
    12121 caggaatgcc gccgcaccag tagaagaacc agccaaacgg gctgcggaac aattccgctt
    12181 tgcccatgta gcgcacgtct gctttcaggc ggaagatgac ccccagaaac aggagaaaat
    12241 cccagttcga ggtgtgaggc gcaccaatga tgatgtactt tgggaggtcg ggtaatttcc
    12301 cttccacgcg ccagccagca aggcgcatgg ttgagttgct caaaaaacga agaagcggac
    12361 tgatgatggg ggtgttgaag atggtcagtt tcatggaata taaaaccctg tcaacatgga
    12421 aatggcacgc cttgcagtaa aatacctgca tggctttgaa atcttccctg cgctccatta
    12481 tctaccaaac tgaaaaaatt gcacagcgtt tgcgttttgc atcccagcct gccagcctgc
    12541 ctgtattgct cggcatttcc tttccgaaaa gcggcacgca tttgcttgac cagattttgc
    12601 ttggtttttc caacattgca ccttacgcca aacgcgtaca ttccttttat gccgaatacg
    12661 aaggcgaaag cggaaaaaaa cgcgaacctg aaaaagcgct cgcctggctt gattcgcttc
    12721 gccccgccga tgttgcatcc gcgcatctct ttgcccgtcc cgaagccatc actcgtgtca
    12781 tctcgccaaa attcgcgcca tacttcatct tccgcgatcc gcgtgatgtg gtggtctcgc
    12841 atgttttcta tgttaccgat atggaaaccc gccatgtcca ccacgatttt tatcaatcgc
    12901 tccccgattt caacgccagg ctaaaagcaa gcattctcgg acgccccgat tcgggcatcg
    12961 agtttccaaa cattgcagac cgtttcgccc cgtatctcgg ctggctcgac caaaaagaag
    13021 tgctgacgat tcacttcgaa gatttgatcc acaaccgcga agcgaccctg acctcaatca
    13081 tggatcacct cctcagccgc gtctcgcttc ctgtcacccg acaactgatt ctggattccc
    13141 tcgaaacatc catcaacccg aaaaaatccc cgaccttccg ctcgggcaaa acaggcgaat
    13201 ggaaaaaaca ctttacagat gaacacaaac agatattcaa agaagtggca ggagatctgc
    13261 tcgtgaagct gggctacgaa aaggacggta actggtaaac cgcaattacc aattaccaat
    13321 taccaattac caattaccaa tgatctccat catcatccgc gcctataacg aagaaaaata
    13381 catcggacgg ctgctcgaag gcattaaaca acagaccatc caggatgtgg atgtcatcct
    13441 cgtggattca ggctccacag actcaaccgt cgccattgcc gaatcatacg gcacgcggat
    13501 cgtgagaatc cgtcccgaag aattcacttt cggacgctcg ctcaattttg gggttaagga
    13561 agcgaagcgc gagttcatcg tcatggccag cgcgcatgtg tatcctgtct atcccgattg
    13621 gctggagact ttgcttcgtc cgtttcaaga tgaaaaagtg gctctcacct acggcaaaca
    13681 acgcggatac gaaggctcga aatactctga acatcaaatt tttcatcaat ggtatcccga
    13741 tgacagtaat gcagaccaga cgactgcgtt ctgcaacaat gccaatgccg ccattcgtaa
    13801 aagcctgtgg ctgcaacatc cctacgatga gatgttgacg gggctggaag atttggagtg
    13861 ggcgaaatgg gcgaaagggc aggggtataa gatcgcttac gctgctgaag ccgaaattat
    13921 tcatattcac aacgaaacac cccgtggagt ctataaccgc taccgccgcg aagcaatggc
    13981 tttgcggaag atctaccccg aagcccattt caacttttac gactttctgc gcctcaccac
    14041 aaccaatatt ctgagtgacc tatggcatgc cgcgcgcgaa cgggttttgc tgaaaaatat
    14101 ttcatccatc ttttggtttc gctacatgca atttcacggc acccgcatgg gtcaccgcga
    14161 aacaagcctg gtaacgccac agttgcgcga gaccttttat tatgcgaggg aaagaaagat
    14221 gaaagatgaa agaaaacggg atgtggagcc gattcggtat aatagtgaat agtgaatagt
    14281 gaataggaaa tagtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttttc
    14401 acccgcttta ttgttacagg agaaccatga aatccaaaac cattgttctg attcacggca
    14461 tgtttatgac ctccctttgc tgggagaagt ggattcctta ttacgaagcc aaaggttacc
    14521 gtgtttttgc acctgcctgg cccgggcgtg agaaatctgt ggaggagctc cgcaaagcac
    14581 accctgattc gacgcttgca aagttaaagc tcaatcacat tgtcgaccac atggaaacct
    14641 tcatcaaagg tttggatgaa aagcccgcca tcatcggtca ctcgatgggt ggcctggtcg
    14701 tgcaattgct gcttcaacgt gatctggcag tggcaggcat tgccatagac cctgcaccac
    14761 cggcgggcgt attcaccacc gaattctcat ttctcaaggc gaacttcccc gccattaatc
    14821 catttttgct taaccaacct gtgcaaatga gttttgagca ttttcaatat gcttttgtca
    14881 atactttgcc tttggatgaa caacgcgccg cttatgaccg ttatgttgtc cctgaatcgc
    14941 gcggtgttcc cacctcatct cttggggccg cgggcaaaat tgatttcagc cgcctgcatc
    15001 gtccgctctt gatcacggca ggtgaaaagg atcacatcat ccctgcttca ttgaaccgct
    15061 ccaattacgg aaaatacaaa ggccccgccg tcacaaattt caaggaattt gccgggcgcg
    15121 atcattttct gatcggttcg cgtggttggg aggaagtggc agattactgc ctcgaatggc
    15181 ttggaaagat tgaataaatc catttttgaa acacggaatt cttaattgtt ttctctgtgt
    15241 ctcagtggtt aatctttcag gccttctgta aagatcggcg atttacatgg aatacagtca
    15301 ccttgagggt ggctgtatgt ttttcggtat aatcatctta tgtccaaatt gatccccatg
    15361 acccaaactg aatttgatgc gttccttgag caggtggttc cagactatgc tgccgacaac
    15421 gtcaaagctg gctattggag cgaagaagaa gcgttggaaa aatcacgcag tgaatttatg
    15481 aggcttctac cccagggttt gcaaagcgag aatcattatc tctacacctt gtatgacgag
    15541 gatcaggctg tcggccgaat ctggatgcgc gccaattttg accgccccac aaaaagcgga
    15601 tttgtcttcg aactctgggt tgatgaacaa ttccgcggga agggctacgg caaacaagcc
    15661 atgctcctgc tcgaagaaaa agcccgcgaa atgggcttga aatcgattgg cctgcacgtc
    15721 tttggataca ataccgtcgc acgcggctta tatgaaaaac tgggctacga agtatccagc
    15781 ttgaatatga gcaagaatct gtaattcact ccatgccttc attttccttc aaacctgcct
    15841 cagagttttc cattccccaa ttagccgacc tgcttacacg cggctttgag gggtattttg
    15901 tccccatcaa catcactccg cccgttttgc tggccatgat gcgccgcgat ggtattgacc
    15961 tcaccgaaag ccgtgtactc ctcaaagatg gcggggaggt tggcatttct ctcatcgccc
    16021 gccgcggctg gacaagccga ctcgctgcga tgggaatcgt atcttcggcg cggagtggcg
    16081 gcgcaggcac ctgggccatg caaaacctca ttcaagaagc caaacagcgc ggtgaaaaag
    16141 aaatgctgct tgaggtcatt gagcagaata ccgctggtgt gaagttgtat gaaaaagtcg
    16201 gcttccaaaa aatccgcagg ctggtggggt acaaactcga aaacccgaaa attgaatcgg
    16261 gtgaagaact cgaagagatc gacattcgtg aactggcgcg catggtcaca tatcatggct
    16321 tgaatgatct gccatggcag ctttcgggca caacggtcat gcatttcacg ccgccttcgc
    16381 gcgccttccg cctcaatgat gcctactgcc tcatcagcaa ccctgagatg aacgacatcg
    16441 cgatccagtc ggtgctggtc aaggcccgtt cacgcggggc aggcctgagc gcagtattga
    16501 tgcgctcact ttttgcgaaa ttccccaaca aggtctggca tgtttcaccc atctatcccg
    16561 aagaaatggg atttatcttt gagcacgttg gcatgacgcg cgaagatatt tcacagtggc
    16621 agatgtcact gactttgtaa gacgcagcac ccattatggg tataaaatag ggggcatgaa
    16681 acttgccgcc ctagtcccca tgcgacacca tagccagcgt gtgcctggca aaaattatcg
    16741 cccgctcgcg ggcaaacccc tttttcaaca tatcgttgaa accctgcttg ccgtacctga
    16801 gatcgaaaca gtgatggtgg atactgactc cgagcctgtg atggagggag tgcgccgcct
    16861 ctttccaacc gtaaaactga tccaacgtcc cgagcatctc cgcgccgacg acatgccgat
    16921 gaacgaaatt ttgctgcacg acacagccca ggtgcaggcc gatttttatt tgcagacaca
    16981 ttccaccaat ccgctgttga aaccgcaaac cgtctcacgc gccattcaat cgctgtttac
    17041 caattatcct aaaaatgatt cccttttttc agtgacccgc ctgcaaacac gtttgtattt
    17101 tcaagatggg cgtgccatca accacaaccc gctggagttg attcaaacgc aggacctgcc
    17161 gcccgtgtac gaagaaaatt cctgcctgta tattttcacc cgcgagaatt tggaacgcaa
    17221 acgccatcgc atcggtgata ctccgctgat gtttgagatc gacgccgatg aagcctggga
    17281 tattgacgaa gaactggatt tcgagatcac cgatttttta atgagaaaga gaatgtaatg
    17341 tcattgcgag ccgcgccatt gctctttgcg gcgaagcaat ctccactttg accatgaggt
    17401 tgcttcgggc ggacgatcac cgccctcgca acgacatgaa atcacatgca aactaaaaac
    17461 gaaaaatcca atccattctg gctgagtgcg ctgatcattt ttgtggtctc tgccattgct
    17521 tatctgccgc tggcgcgtaa attcggctac ctcaacgatg actggtacct gatgtacgac
    17581 atgtgggtga aagatcccag tttcttccat gtcattttca gcggtgaccg ccccgggcgg
    17641 gcgctggcga tgattccgct attctcgctg ttcggattcg aaccgcttta ttatcacctg
    17701 agcgctttcc tgttccgctt tctcagcggc gtgagcttgt attggacttt ccgcatgctt
    17761 tggccgtgga agcggtttgc cgcattggcg gctgcgcttt tgtttgcggt gtatcctggc
    17821 ttcctctcac agaccaatgc cattgattat caatcgcata ttttggggtt gttcctggca
    17881 gccctgtcgg tggccttgac cattaagtcg cttttgacgg tcgagcggtt tccgcgtttc
    17941 ttttttatgg cggcttccat tttggctggc tggggctatt tgagccagat ggaatatttc
    18001 atcggcatgg aggcttttcg cttcggttgt atctttattt tgatgtggcg gacacagggc
    18061 gcttcctacc tgcagaaggc ggggaattgg ttcattgcca gtctgccgtt tttgctggtg
    18121 gctggcggat ttttgatctg gcggttgttt ttctttgaat ctgaacgcaa ggccacagat
    18181 atcggtttgc agatctcgca gttgacctct tcgccgctga cgttgttgtg gtggttgaat
    18241 tatctggtgc aggatatggt cagtgtgacg ctggtggcat ggggattgcc cgtgtatgtg
    18301 ctggcgtttc cgatgcggct gcgtgatgtc atgtccgctt ttgggtgggc gggacttgcg
    18361 gttgcgctga tggtgttggg gatgaatcgg gtggataaaa gtaaatcgga agcagaagca
    18421 ggcccgagtt tgagtcgtga agcgctgatt ttgagtctga tcagcatcgt gggcggactc
    18481 ctgcctgtga ttttggtcaa ccgacatgtg acccttcccg attattcgcg ttacaccttg
    18541 atcgcttcgg tgggggcggc cctgcttttg gtgacgatca ttgaaaagat ttcggcgcgg
    18601 gttgcgctgc tttcattctt tgtggcaatt gcggtgctga cgcattacgg caacgcggtc
    18661 cgtgcggtgg atgaggcgca ggcgacgcgt gatttttggt ggcaggttgc gtggcgtgtg
    18721 ccgcagtttc agcctggggt gacgttgatc gcttcgtatc cgggcagccc gttgagcgag
    18781 gattatttca tttggggtcc tgcgaatttg atctattatc ctgaaaagca ggatgcgaac
    18841 ccagttgaga taaaattgcc cgctgccgtg ctgaccgatg acgtggtgct ggagatcgcg
    18901 acgaatggcg gtgtggaaac cccgctgcgc cgcggaaatt atctggaacg tgattttgga
    18961 aatgtgctgg tgatgatcca atcacggtca aatggctgtg tgcgcatgat caatggtgat
    19021 gtgcctgagc tgtcgccgct ggaccagcat cggctgttgt tgatcgcgcc gttctcgcgt
    19081 ttggaaaatg taaaaaccga aggcgagttt ccaactccgc cagcggttgt atttggcagc
    19141 gaacctgagc gtggctggtg ctatttttat cagaaggccg atctggcacg tcagcgcggc
    19201 gattgggagc agatccctgt tttgttgaaa gaggcgttgg atgcaggcca ctaccccgaa
    19261 gatgcgctgg agtggatgcc tttcctgcag gcttcagcgg tgctcggtga tgtggagcag
    19321 gtgcgcagca cagccaaatt ggtcaccgtg gataaattcc tgcgccttca agtctgtaat
    19381 atcatgacgg atttcatgga aaaagaaacg ctgaatgatg aagtgaaatc tgtgatcgag
    19441 catacgattt gcaagtaaat atctgaaaat ccaccacaaa ggagacaaag ggtcacaaag
    19501 ggttttcctt tgtgttcctt tgtgaccctg cgtggtaaaa acgaggtcac tttattaatg
    19561 aaaaagtttt ttagcagttt cttcaacgcg ccgtggtatc cgatcgtgat cagcgcgtac
    19621 cctgttctgg ctttgctttc ttcgaatgcg gggcaggtgc aatcgaatac gggaatacgt
    19681 ccgctgttgg tttctgtggc gttcggcggt ttgttgtttt ttatcgtctg ggtgattttg
    19741 cgcaggcagg tgcatcgcgc cgcgtttttg accaccctgc tgctggcctt gttctttacc
    19801 tatggccatg cctacatcgc cgtggatgaa aaatggcccg atgctgggta tgcccaatgg
    19861 ctggccgtgg tttgggcgat ccttttggtt ctggctgtct tttgggccac ccgcccaaag
    19921 tggacctttg tctctgcggc ggcgaatttg aacacggttg ctctggcttt ggtcatcatg
    19981 gctgcggcgc aaattgattt ttcgaatggc atgggcaagg ttcatcgggt ggctttggag
    20041 aaggctccca ttcaggaaga cctgaccctg cccgagaatc cgcctgatgt gtatttcttt
    20101 ttgctcgatt cctacggccg ctctgacttg ttgtacgccg cctatgggtt tgataacagc
    20161 gattttgaaa accaactggt ggaccgcgga ttttatatcg cctcgtgcag tcagagcaat
    20221 tacgtccgca ctgagatttc gctggggtcc tcgcttaaca tggcatattt gcagggattg
    20281 gatgaaacct ttacccccga tagcacagcg cgccgcgtgt tgtgggatgc gctcaaacac
    20341 aatgcggttc gctacaactt tgaaaccctt gggtacgaaa ccattaactt tgcatctggc
    20401 tttgcctggt tggagatcac cgatgtggac cattttatga gtccgccgcc tgcttcttcg
    20461 gggatgaatg aattcgaagg cttgttcctg cgaaccactc ttgcacgata cgcacaggac
    20521 ctcggctggg tggaccccga ttacctgatg ggcgtgagct tccgcgaccg atttaactac
    20581 atcttcaaca gcatggatga gatcacagcc atgccacagc ccacctttgc atacatgcac
    20641 ctgatctcgc cgcatccgcc gttcgtgttc gaccccgaag gtaaccccac ctatccgccc
    20701 gatttttgga acgaccagcg catgtatccc gccgatatgt accagaaggg atatgtgaat
    20761 caattgcaat atctcaacaa gaacatgctc gaagccgtgg ataccatcct tgcgaattcc
    20821 gatacgccgc cgatcatcat cattcaaagc gatcatggtc cgtggttgca gcccaaagac
    20881 aaacgcatgc gcaacttcac agccattctc atgccaggtc atgaagatgt gctttatccc
    20941 accatcactc ccgtcaatat tttccgcgtg gtcttcaatg cctacttcgg cgggaaatat
    21001 gatattctcg aagatgtcag ttatttttcg cccgtgccaa agttgtacga tttcagcgaa
    21061 ataaaaaacc catgcgcaaa tggaaattga tgaatgaaaa aaacagtcct tctcaccgcc
    21121 ccttacatgc ttccgtttct cgaacgtttt cgacccgaat ttgagaagta tgatcttgac
    21181 ctgatcgtac ccgatgtgga agaaagaatg gaagaagccg acctgctcaa atatgcaggc
    21241 cagtttgacg gcgccgtttg cggcgatgac cgttacacag cgcgcgtcat cgaagcctgt
    21301 tccccgcgtt tgaaagtgat ctccaaatgg gggactgggg tcgattccat cgacgccgaa
    21361 gcctgctccc gtttcaatgt cattctctcc cgaacgccga atgccttcac cacccccgta
    21421 gccgatacgg ttctggggta catgctggcc tttgcccgca gaggcccgtg gatggacagg
    21481 gaaatgaaaa acggcaagtg ggaaaagatc ccaggcaagg cactgagcga gcttacactg
    21541 ggaattatcg gcattgggaa tatcggcaag gcggtcaccc gccgcgccaa agccttcggt
    21601 atgaaagttc tcggcaccga cattgtggat gttgaccatg tcttcgtcaa tgaatcgggg
    21661 atcgaaatga ccgacctcca ctctctgctt tccaattctg atttcgtttc agtcaactgt
    21721 gatctcaacc ccacatctca tcacctcatc aatgcaaaaa cactttcgct gatgaagaac
    21781 accgccgttc ttatcaacac cgcccgcggt cccatcgtgg aggagcctgc ccttatcgcc
    21841 gcactccgtt caggtcaggt tggaggcgcg gcgctggatg tcttcgagta tgagccgctg
    21901 cccaaggata gtccgctgtt gaagatggat aatgtcatgc ttgcgccgca caattccaac
    21961 tcaagcccca cggcctggga gcggatccat tggaacacga tcaaaaatct ggtgacaggg
    22021 ctgggactcg aatacaaggg ctgagttctt catgtccaag ctccgctctg cgcttccgta
    22081 tctttttatc tttctgctgg ccgcgttggt tctcgatctg gggaatccgc tttttgacaa
    22141 accagccagg gatggtggtt tttttctgta tgcgggtagc cagataataa gcgggaaaat
    22201 tccctacgtt gatttttggg attcaaaagg cccagccatt ttttatatca atgcgttggg
    22261 cttgtttctg gggcagggtt ctcgttgggg tgtgtggggc gtggagtttg tcttnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnggcctg cggatcgtgc ttggctacgg
    22441 aaattacacc gaggaatatg ccctgctgtt caacgcactg gctgtgtatt tattttttgt
    22501 tgtggatgaa aacaagggtt ggaaatattt tgggatcggg ttgctgttcg gcatcagctt
    22561 ttcttttcgt gccaataata tcggcgggtt gtttgcggtt ttgattgcac ttgcgcttgc
    22621 gggttttgtt ggaaatcttg caaaaacccc gaaccctaac cacagagaca ctgagtcacg
    22681 gagatttaat ggctttttcc ccggtgtctc catgtctccg tggtccaaaa gtttttttat
    22741 acgacttcta ttcgttcttg ctggtttcgc cgctccgctt ttgctgtggg ttttgttttt
    22801 ccagatccat ggcgcggcat gggagatgat cttcgcttcg atcattttca atttttccta
    22861 tgcctcggcc aaaagcaggg aatggttgga tatcttcggc ggctttggcc ggtatggtat
    22921 gttgtgggtt ggatgggtgg ctgctttggg gtatgtggta aatggatatc acgtgcttcg
    22981 caaggcgctt catcgcaatc cgctcacgct ggtggattgg ttcctgttca catggttccc
    23041 gttcgaaatt cttttgagca atctttcagg caggaatttt tcgcattact atattagttg
    23101 ggcgctggcg gttgcggttt atggtgcggc actttttaca agcggcacag gctttagtct
    23161 gccaaaatca ctgaccgccc tcactacggg tgtccgtaca tcttttttgt atgacgggat
    23221 ggtgttgttt tttatcgtgg ggttgctgct gttttccact ccgtctataa aaagatatgg
    23281 tgagacggtt tcaagatcgt tttcagggca aagccttgaa tattctgatc cgcttgtgga
    23341 tgcgattcgg gcgaacacac agcaggacga cctcgtatta acgtggtatc ctgaaacgac
    23401 tctgaatttt atggctggac gtgcctcgcc tgtgaaatat gtctactatc cgctgtttct
    23461 tgaggggaca ctcccgcccg gggctgagac aagttacatc gaaggcctga ccagtcgtcg
    23521 gccagaaatg atcgtggatt gttcgcgggc ggtggatgcg attccgtcgc tggaccctgc
    23581 tgcgcgggag gatcaattct cgaagccggg agtcaaaaag aagatgtatt ttcagcctgg
    23641 catggaagag attttcaatt tcgtggctga gaattaccac gtggaaactt ccgtggatgg
    23701 ttgtttactc tttcgattga atttggaata aaaaatggac acacaaaaat tcgttctcat
    23761 taccggcgcg gcaggtggaa ttggccgtgc aactgtcaat ctgttctttg aaaaaggctg
    23821 gcatgtggtc ggcattgacc gatcggattt cggcgaaggt tttccagcca acggcctttt
    23881 cgtgcgttcg gatatctccc gcccggagga tatgcaggcc atttttgaaa aggtcagtgc
    23941 tgtgacggat tcgcttgatg cgctggtgaa caatgccgcg atgcaggttg cgaagccgct
    24001 ggtggagacc accgttgaag agtgggatgc agtgatgaat gcaaacctgc gctcggtttt
    24061 tcttggcgtc aaacttgcgc atccgttgtt gaaggcgcgg ggtggggcgg cggtggtgaa
    24121 tgtttcctcg gtccatgcga ttcagacttc ggccaatatt gccgcctatg cagcttcgaa
    24181 gggcggcttg ctcgcgttga ctcgtgcaat ggcgatcgaa tttgcgcccg ataatatccg
    24241 agccaatgcc attctgcctg gtgcggtgga tacgcccatg ctgcgggcag gcctcgggcg
    24301 cgggcatgtc ggtcagggtg atgtgcagga caggcttgat aacctcgccc gcaaaacggt
    24361 caacgggcgg gtgggtacgc ctgaagagat cgcccatgcc atttacttcc tcgcggataa
    24421 ccagcagtcg tcttttatga caggtcaggc attggtggtg gatggcggcg caacagcgag
    24481 attgagcacg gaataaaaag caactgtgca aaggcagtga acgcacgaag attccttctt
    24541 ctttcctctt cgagcctttg caaaaacagg tatcgcaccg atgaaaagac gaacacgaaa
    24601 aacaaaattc ctgaccgatc gcgcctatgt tcccgcatcg cgaaaaacgc ttgtgcggat
    24661 tctgttcttt atcaatgcgg cgctctggct tggttatgtg gtttacattt attatgatat
    24721 ggcagtcgtc aacaagaacc tgacctcggc ggatatcgtg tcactttttg tctttgccaa
    24781 ttcggtgatg atgttcgtca gcgggtggca gcttgcaaag aacgaaagtt gggcctatta
    24841 tcttgccatg gtcgtgattg gcattaatct gcttttgacc ctgctgaaca ttctggatct
    24901 gtttttcctc ggcatatttt tgctcgacct gttcattctc ttgagcctga tccccattcg
    24961 caaacactat ctttcgaagc catgaaaaat ataaaaactg tgggttctct gttttttgtc
    25021 actgcctttg tgctggctgt ttttggcggg gcgtcgtttt cacgtgccca tgccaatccc
    25081 atttacaatt tgtatgcggt tttgatgttt gtcgatgccg ctgtcatgct ggtttgtggt
    25141 ttgtacatca atagacatac tcaggttgta tactggtttg cagtggcagc cctgactcta
    25201 aatatcgttt tgaccatctt cgatcagatc ggtcttgttg acattctttt tgcagcgttg
    25261 aattttgtca cccttatggc gctggtctca tctcgcaagg aattttctca tcaatgaaac
    25321 agacgataaa atcgcttttc cctgaatcga ctttcaatgt cgtgcgcgac gcctacgatg
    25381 ccgtccgccg catcccgcaa ttgcccgacg cgtacctgca tccgtggcgg cgcgaaagca
    25441 tccgccgcct ggccgcgctc aaggatattc acaaaggcca gcgcgccttc atcattggca
    25501 acggtcccag cctgaaacag accgacctca ccaaattgaa gaacgaattc accttcggta
    25561 tgaatcgcat ttatctgctg ttccccgagc tcggattcca caccacgtat ttttgctcca
    25621 tcaacgacct ggtcattgag caattcagcg acgatatcct cgcgctgccc atgccgaaat
    25681 ttcttgcatg gcgttcgcat cgtcactttt ccaaaaacct gcaacttgca aacattccaa
    25741 cgtttcttta caccacgtac acaggtccgc gttttacccc tgatgtgcgc agacgggtgt
    25801 gggagggtgc aaccgtcacc aattttgcgc tgcaactggc ttttcacatg ggcttcgaaa
    25861 aggtcattct catcggtgtg gatcacaact tcgcctcgaa aggtgacgcg aacaaaaccg
    25921 tcgtttctga gggtgatgac ccgaatcatt tttcgccgaa ttattttgcc aaaggtttca
    25981 aatggcagct ccccgatctc gacacctccg aaatcggcta cacctttgcg cgtgaagcct
    26041 atcgaaaagc aggccgcgaa gtgctggatg cgaccgtcgg cggcaaactg accatcttcc
    26101 ccaaagtgga ttacaactcc ttgttttgat ttgcgattga tcccctccat gaaattcatc
    26161 ctgtatcatt cacccttcct cctttgaaaa tgacgcctcc cacccgaccc gcaaactggt
    26221 tcatcgcctt tatcattctc ctcgccttgg gcggannnnn nnnnnnnnnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnctctt ctggctggcg gcgggcattg ccttgtttga cttgatgcgc
    26401 cgtgcggttt ctccgtgggc ggcggtggtt gcgattgctt tttattttgt cctgccgttt
    26461 tcggtgcaag ccagccgctc ctttcaaccc gatccgctca tgacggcggc atttgtggca
    26521 gggatctatt tcctctatcg ctggtccgaa gaacagactt ggaagtgggc catcctcaca
    26581 ggtgttttct ttggatttgc cacattcgtc aaaattgtca tcgctttttt tgtgggtgct
    26641 gctgcgattt cactggtttt attcaccctc aaaaaggatt tttggaagtc gaagcaagtc
    26701 tgggtgatgg ctgcgatcat ggttgtgcca gccttccttt tttacatttt gcttaatcag
    26761 ggccgctcta ctgaatactt cttcgcatgg acggtcacgc ttatcaaact catcaccagc
    26821 gcagattttt attccaagtg gctggcgttt gtcggcagca tttttgggtt gacgatgatc
    26881 tttctcagcc tggcaggggc gttgatcgca actccgcgtt tgcggtggtt gttggtcagc
    26941 ttgtgggtgg ggtatgtgct ttacgggttg accctgccgt tccagatgta cacgcacagc
    27001 tattatcata ttcaactgat tcccatcatt gcgcttggtt tggcttcggc gctgaatccg
    27061 ctgattgaaa gcgtggcagg tcaaggtcag gtgggacgcg tgggtgtcat cgcgttgatc
    27121 gtcgttgtga tcgggtatca atcctgggtg gcgcgttcag ttttgatcgc ggaagatttc
    27181 catcatgaac ctgctttttg gaaacaggtt ggcgatgcga tccctgcaaa caccgatgtc
    27241 attgcactca cgcaggatta tggctatcgt ttgatgctgt ttggctggcg caaggtgagc
    27301 ctgtggccgt tgagtacggg cttgagcgag gcgcgcggcg gaaacaccga tgtggctggc
    27361 aacttcgcag acctcactgc aggcaaggaa tattttcttg tgaccgcaca gggacagttt
    27421 gataaacagc ccgagttgaa aaagattttg tcgcaatatc ccattgccgc gcagggtgag
    27481 gggtatgtgt tgtttgattt gcgaaaatga ccctcgtttc catcatcact ccttcctaca
    27541 atcaagccgc ttaccttgag cagacgcttc tttccgtgct gaatcaggat catgctccga
    27601 ttgaatacat cgtcgttgat ggcgcttcga aagatgcaag cgtggagatc atcaaaaaat
    27661 attccgaccg attggcgtat tgggtttcag aaaaagacgg tgggcaggcg gaggcgatca
    27721 acaaagggtt tgcacgtgca acgggtgaga tcattgcatg gataaattca gatgattatt
    27781 atttgcccgg tgcggtgagt gcggctgtaa aaatatttga atccaatccc gatgtggtgc
    27841 tggtttatgg gaatatgctc gctgtggatg agcacgggaa aaccttcaac acactcacct
    27901 acaagcaatt gaccttggaa gacctgcttt gctttcagat catcggtcag cctgccgttt
    27961 tcatgcgccg ctctgccctg caaaaaacgg atgggcttga ttccactttc cattttctac
    28021 tcgatcattt tctctggatc caacttgccc agcatggaaa aatcttacac gccgatcaaa
    28081 cctgggctgc ggcgcgttat catgccgaag caaagaaccg tgccaaagcc gctgaatttg
    28141 ggcgcgaagc ctttcgtatt ttggaagccg tcgcacagga caagaatctt gcacctgtcc
    28201 tttcaaaagt ggagcgtcgt tcccgtgcct cggcacatcg cgtgaacgca cgctatctac
    28261 tggatggcgg tcagcctgcc gccgcactgt ccgcgtgggt acgcgctttt ttcatccacc
    28321 cgccaacggc cttggcacgg atgaatcttt tcgtctctgc gattttgaat ttgttcggtt
    28381 tggggaagtt gcgctcgatg gttctgcgaa tgcgcgaggc acggcacaga gagtagaaac
    28441 tactgacaag aaaatcggtg acagatgatc atctgtcacc gatttgtctt ttaaagggtc
    28501 ccgccgctca tcagcatggg ggggacatgc aaatcaagcg gcgggggaca cctagatacc
    28561 agtaaaaggg agggcaggtg tcgatggcta gaatatacac ccgaattgtc caaataacat
    28621 taatctaaga ttagaaagtg gagcgacttt gtcctattta gtacttttgt cctggaagat
    28681 ttcgagccac tcatccactt cagcggctga aaggcgttca tccgtcgcgc ctggcggggc
    28741 ggagttgata acctgttcca ccaatttgga aaaagattca gatgaggtaa attgggcccg
    28801 ggcggtttgt gcggcacttt gcacttctct gtcagaggag accacggtcc attgttttgc
    28861 ggttttcccc atatttttca aacgggcacg gatggcgcta tcggccgttt gaccggctcg
    28921 gacaaaatgc gctgtgattc cgctgagctt gcgagttccc gcctgcccgg gtggggcgcc
    28981 atcgaaatac acttccacgg tttcgcgttt caggcgcgca aagttttgca gcagacgtgt
    29041 caactttact tcatcgtcag atgaatcgag tcttaatccc acttttggaa tcaggttatg
    29101 tccatcaatc aaatagggca tagcgtgaat tttacccttg aatcaaggcg ggcacgggag
    29161 taccgtgccc gcatcctttg tgctgtggtt tggcattaag ccggttcaag cactttggtc
    29221 attacattga acgtgggtgg gttttccagg gtctttttta ccttgcataa ttccgccgag
    29281 cgtacgagtg catcgtaata ttgcgcaggg aaggaagggg ggacctgaat ttcaagatca
    29341 atttcgctca ccatcccatt cattgggttg gcgtggttgc gctgaatgat gtgaatccca
    29401 tcggtgggca gattgcgctg gcggcaaaag ccgagtacat aaatccctgc acaggtcccg
    29461 atcgaagcaa gaaacacctc aaatggcatt ggcgcagaat cggcgggcgg ctggtctgtt
    29521 gctatggtat ggcctcgaaa atgagcgtcc actcgtaaac cgccgggaaa atcaattaac
    29581 atctccatgg ggagcctcca aagtagttag tttttgtcac aaataattga caacccgcgc
    29641 acaaaaaata ttacctcttt ttagaccaaa atccgtatat caagtgtcct caaaatccac
    29701 acagcctgag gatgtataat tgcactactc agaaaaggag acacaatgac agaaaagaaa
    29761 aacaccaaca ttacagtttc tccgcaaagc ggaatgattc acaaccttgt tgtgcgtacg
    29821 aaacttgttt tacgcttgct tggtgataaa cgcgtaagtt catgggcaaa acttatcccg
    29881 gttggttcga tgatctacct tctttcgccc atcgacgcga tcatgggagt cccgggtttg
    29941 gacgcattgg atgacgccgc catcgttggc ttgggatact actttttcat tgaactgtgt
    30001 ccgcccgatg tcgttcaaga gcatcttcaa gcgcttgagg gcaccaacga accgccccaa
    30061 gcccgcgaag atgtgattga tggtgaagct actgaagtaa aagaataaat gagacgattt
    30121 ttgattctat taactctgcc aatccttgcc tgtgggggat tggcagagtc tgttgtagcg
    30181 acgattacgc ctcaagccgc gccgacaatt gctgcgcccc cacaaacaga atctccccct
    30241 accgttgact ctgccactga gcctccagtc atttcaccta ctgaagtgac cgcggccaat
    30301 gtcaatactt ttcccaatac tgacaactat caatggaccc tcatcgccag tggtctcagc
    30361 cgcccagtcg atatccaatc ggctcatgat ggtacgggca ggctgttcat tgtcgaaaaa
    30421 tatggtgtca tccgtgtata tgaaaacggg caactgcttg accagccatt tctggatatt
    30481 tccgaccgcg taggcgatga gtcgaatgag caggggctgt tgggtcttgc ttttgtgccg
    30541 aataactctg gggtattgtt cgtcaactat accgataata acggcaacac ggtcatctct
    30601 cgtgttttgt gggatcaagt ggcaaacccg ttgagcgaaa aagatctcct gcatgtcgaa
    30661 cagccctacc caaatcacaa tggcggggcg ttggcgatcg gccccgatgg ctatctctat
    30721 atcgcattgg gcgatggcgg gctggcgggc gatccgcaca agaatggaca gaacaccaat
    30781 tccctgcttg ggaagatctt gcgaatcgat atcagcgacc tgaataaccc caataactac
    30841 agcattcctg cggataaccc ttttggcaat gaggtttggg catacggctt gcgcaatccc
    30901 tggcgtattt cctttgaccg tgtcactggc gatctatgga tcggcgatgt ggggcagggc
    30961 gattgggagg aaattgatta tctccccgct gggtcggcgg gtggcgcaaa tttcggctgg
    31021 tctgtaatgg aaggcagtta cggatacgat ggggtggctc agcctggcat gttgctcccc
    31081 gcggcggaat atagccataa cgaaggcggg tgctctgtta cgggcgggta tgtctatcgc
    31141 ggcgccatga ccgagtggaa tggcatttat ctgtatggcg attattgcac aggaaagatc
    31201 tgggggctga ttctgtccaa tggtcagtgg cagtctcagg tcctgtttga agcgggtgaa
    31261 accatcacca cttttggaca ggatgaattt ggcgaaatct acttcgcaag tgattcgggc
    31321 aatgtctatc gccttacaaa aaaataagga catatcacat ccacaatagg tgttttgtca
    31381 tgggagtttg tgacgcctgt cactggcatg gcaggcgttt ttgttttaaa ctgggttcat
    31441 cagagaacgg aggcagatat ggcagaaaag atttggcaag ttgaaaaaat caaatactgc
    31501 gaacatgttg ggcacgaagt tgcgatcgag aacgaagttg tgtacccagc tgaacatctg
    31561 cctgaacagt tgccgcgcat tgtagcccac cgctgctcaa acgcgatcga atgtaacctg
    31621 atcgataagg caacctgcgc cctgtgtggc acgaatcctg atcttgaccc tgtgtaattg
    31681 agcaatcgca aattgctctt ccaaactctc tcctccctct gagttggtct attggctgca
    31741 ccgcgtgaga acgcggtgta tgccgttaaa aaggggatga catttctaca gttctttcaa
    31801 aaacctctcc acgctttcat cagcagggga gttttttact tcctgtggtg ttcccacctg
    31861 tttcaattcg ccattgatga tcacagccac gcggtcgccc atttgtgcgg cttctttgag
    31921 gttgtgcgta acaatgatgg tggtgcgatg gtcgtgtgac agcagtgtgg aaaggtcctg
    31981 taataactgc gcccgtgttt gtggatcaac cgccgagaac ggttcgtcca tcaacagcaa
    32041 ttcaggatcg aggacaaaag cccgtgccag actcaccctc tgcgcttcac cccctgacaa
    32101 ttggcctgcc cgtcgcttcg acagagaatc cacccccatg gatctcatcc atttttctga
    32161 acgcatctgc gattcaggtt tatccatgcc gcggaatttc aaaccaagag ccacgttttc
    32221 ctccaccgac atatccatca aaagcggatc ttggaaaaca aaagcgatct ttctgcgata
    32281 ttccaaatca ttccattcgc gcagggattt gccgccgtat ttcacatcgc cgctggcggg
    32341 tctcagtaaa tgtgcgaggg tcaacaacaa cgtacttttc ccagaaccat tggggccaac
    32401 gatcgccagt gtttcgccgc gcggaatatc caatgacgca acatggagtg catcgcgttg
    32461 gttgcgctga actcgcaggg ctctgatctc aatgagattt gtcgtttttg tttcgctcat
    32521 cgtctctgcc ccttttgttg tacccaagtc agggcaaggt tgattaggta ggtgatgatg
    32581 agtaacaagg cgctcaatgc cagtgcgcgc tcgaattccc ctttgctggt ttcaaggaca
    32641 atggcggttg tcagcacccg agtctgcccg cggatatttc cgccgaccat catggatgcg
    32701 ccgacttctg aaatgacaga accgaaaccc gccatgagcg cggccaaaag cggcaaacgt
    32761 gcctcccgcc aaagatgcca gatcatttgc aggcgtgagg ctcccaaccc caaaagttgt
    32821 tgctgcaaac gcggatcaag cgccgccagt gcagccgccg tcaacccaat gaccacaggt
    32881 gcggaaatga tcgtttgggc gatgacaatg gcccacggtg tgtaaatgag ccgcaggctt
    32941 cccaacgggc cgctgcgcca gagggtcatc gccacaacaa ggcccaccac aacaggcggt
    33001 aatgccatgc ccgtgttgac aatgctgagg atgaaagccc gaccgcgaaa attgccgagc
    33061 gccagccatg tacccaacgg caggccgatc agcaggctga cgaacgtggc aatggttgag
    33121 acttgcagag agagggcggt gatttcgaag atttcagaca tgggtgaggg gcgggtaaac
    33181 aagtagacag gtaaacagga ttttgtgccc gtttacctgt ctactttatt tatttagcga
    33241 ttgcaaatta tagccccaaa tcggcatcag ttttatctgc atcggggtag aaaagcggct
    33301 ggccgaattt ttccttgccg aactcggcaa taaccgcctg tgtggcgggg tcggtgatga
    33361 atttggcaaa ggccatggca ccgtcatagt tgaccgccgt ccatttatcg gggttgaccg
    33421 tgatgacgtg gtaaacgttc aacagggcat tgttgccttc gagcaaaatt tcaagttgga
    33481 ggttggcctt gttggagagg taggtggcgc ggtcagtaag gatgtatgca cccttttcgg
    33541 aagcgaccgt aagcgaagcg cccatgcctt gacctgtttc gatgaaccag gcgggctttt
    33601 cagtgcgtgg atcgagttcg gctttggtcc agaaactaac ttcctttttg tgtgtgccag
    33661 agtcatcgcc gcgggagaca aaaggcgccc cagcgttgta gatggcaaca aaagcatcct
    33721 tggggccgag gcctttgatt ccagcagggt cggcggcggg gccgacaatg atgtaatcat
    33781 tgtgcatgac gagcatgcga tctttgccga agccgccatc catgaaagtt acttcggagg
    33841 agggggcatg gacgagcagt acatcggcat tgccttcttc gcccatcttg agggcttcac
    33901 ctgtgccgac tgcaacagtc tgtacggtgt agccggtctg ctcttcaaac atgggtacga
    33961 gcacatccag caaccctgaa tcctgagtgg aggtggtggt agctagaatc aggttggggt
    34021 tggcgggtgc ggcagcagga gcttcggtag gtgcttcggt tggggcctcg gtggctggtg
    34081 cctcagttgc aacaggtgct tcagtggcag gggcagccgt tggggcgggc gatccgcagg
    34141 cggataatgc aatcgccagg atcaagccga gggttaataa aatgttgcgt gtttgtttca
    34201 tcttt
//
LOCUS       DAOQLC010000036        34112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_393_length_34112_cov_24.767038, whole genome shotgun sequence.
ACCESSION   DAOQLC010000036 DAOQLC010000000
VERSION     DAOQLC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 34112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_393_length_34112_cov_24.767038"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PL000_08400"
     CDS             complement(<1..604)
                     /locus_tag="PL000_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNC88959.1"
                     /translation="MATKVLVPRLGEGVDEVTVTKWLKQVGDSINELEPLLEVNTDKV
                     DTEIPAPVTGTILQIIAQEGTPAKVGELLAIIGQPGESVDSGLLMVNSGQLTVDSNTP
                     VKPTTNHQPPTTNYTPSTANNLGFISPVVAKIAAEKGVDLSQVNGTGLNGRITKNDVL
                     NYVESGKSKAANQPANLQTFKPSNLTPDTGSHDTLIKHSSI"
     gene            complement(646..1032)
                     /locus_tag="PL000_08405"
     CDS             complement(646..1032)
                     /locus_tag="PL000_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNC88960.1"
                     /translation="MEKEKISAKNYRELIVWQDAIKIAKAVYLLTGKFPKQETYALAD
                     QLRRAAVSVPSNIAEGQARKSPGDFRRFLHIALGSLAEVDTQLILAQEFGYLSKEDID
                     PMDMEIQNLRKKLYALINSLPSATNR"
     gene            complement(1081..3498)
                     /locus_tag="PL000_08410"
     CDS             complement(1081..3498)
                     /locus_tag="PL000_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNC88961.1"
                     /translation="MPNKKKGTIALPLEKDEIIKDYRLAYQSRQASIIGRREVLSGKA
                     KFGIFGDGKEIAQLAIARAFQKGDWRSGYYRDQTWMFMLGVMSIQEFFAQLYAHGDVT
                     YDPASAGRQMNAHFASRNLYPNGTWRPQTQMYNTASDISPTAAQMPRAVGFAYASVMY
                     RNIKELQDQKDFSINGNEIAFATIGNASTSEGLFWEAVNAIGVLKAPAIITVYDDGYG
                     ISVPNQFQMVKENIGALLKGFERDPFTPPDKVQNGYDHYTVRAWDYTALVETYLTAAE
                     IAREYHIPALVHVIDVTQPQGHSTSGSHERYKTPERLKWEEEFDGLKKMRAWMITNRV
                     ISAPELDSVEKEDYATVEGFRKAAWDAYLAPITEERNQVLDMLDEIAPTSNHVYELRL
                     IRDRLATLPSYSRRDIHSIVHEALRLLRDESHPSKQVLIQWKHENDSVNVERYGSHLY
                     SGTAMKVSEVKPEYSESSPTMFGFEIMNAAFDAALTRDPHIIAFGEDVGKLGDVNQGF
                     KGMQEKYGEFRVTDTGIREATIMGQAIGMAMRGLRPIAEIQYLDYVLYALQTMSDDLA
                     TLHWRTAGGQKAPVIVRTRGHRLEGIWHSGSPMSGILNLTRGMYVLVPRDMTHAAGFY
                     NTLLKSDEPAIVVEVLNGYRVKERLPENIGEITLPLGVPEIIRDGKDVTIVTYGACCR
                     VVLDAADKLSKVGIDVEVIDVQSLMPFDIHGKIVESLKKTNRILFVDEDVPGGTTAYM
                     MQEVIEKQGGYFHLDSPARTLSAKAHRPAYGSDGDYWSKPNAETIFDAVYEMMNEVDP
                     GAYPLFS"
     gene            complement(3616..3966)
                     /locus_tag="PL000_08415"
     CDS             complement(3616..3966)
                     /locus_tag="PL000_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88962.1"
                     /translation="MKSIERRNGYLYLEYAEPYTFENFITISKQVFETCKAEHYQKVL
                     VNVSEMTGKVKALERFEIGVQGALIFRNKVKIAVLYRAEEIDWFAETVSVNRGLNARI
                     FSEMEKAMEWLGVG"
     gene            complement(4039..6024)
                     /locus_tag="PL000_08420"
     CDS             complement(4039..6024)
                     /locus_tag="PL000_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNC88963.1"
                     /translation="MLTLAEKILFVFATLVSLYFTYKGVMKIVGHISSGQGKADWSLL
                     WKRIGDLIVKVGLFQPVFRFRLGPSILHALIGWGFLSFLLINLADLIYAFTNYKLLEH
                     LGIFGDAYRLLADIANVAIIVGILAMAFRRFILRPATLSTRETTLLNPKARTGIMRDS
                     AIVATFIFVHNTMRFLGESFGVALAGHSDRWQPSISAAAGLWSGVDASVLVAGEHIAF
                     WLSIGAVVAFLPYFPYSKHIHLFFAPLNFALKPERKSIGELSYINLDDQSIEQFGAAK
                     MKDLGWEQIMDSYACIMCFRCQEVCPAYNTGKLLSPAALEINKRYHFNGGGTTDVAMT
                     ELISEEAVWACTSCGACVDICPVGNEPMRDILDIRRNLSMMESAFPKQLETAFKGMER
                     NQNPWNVSQGDRMKWAEGMNVPTIEQNAEPEILWWVGCAPATDSRAQKTAQAFAKILN
                     TAGVNYAVLGKNESCTGDSARRAGREDIFFGLASQNVEILNEVAPKRIVTTCPHCLHT
                     IKNEYPAFGGNYQVIHHTQLINELVGSGKISMNLEGDNMKITFHDPCYLGRHNKVTDA
                     PRDTLRSAGVETVEMPRNSAKSFCCGAGGAQMWKEEEQGSARVNITRFNEAKATGAST
                     VAVGCPFCLTMMTDAAKGDGGEIQVKDVAEIVAERLK"
     gene            complement(6115..7089)
                     /locus_tag="PL000_08425"
     CDS             complement(6115..7089)
                     /locus_tag="PL000_08425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC88964.1"
                     /translation="MKQLAETITATDAEIILENIHAMIALVEKEGLLISWNRAFEACK
                     KLFPQAKTLGDFFAPDDRDLIISKLNSRAREHWIIKLPLPAGRSEVVIQCDCHLVPLA
                     NGQMVFIAEEIHLDSAFQEVERLNRQVKLFKIESEQSKKLARRKQVEIEGVMAQAREV
                     AETDALTFLPNRRTIIRELQDEFIRAERYQNQFSISIVDVDHFKTINDTHGHMTGDEV
                     LRQIAIHLRDHVRHPDVVGRYGGEEFLILLPNSGQKAAEEQASRLCQLVREMKIVINN
                     GEVLNVTVSIGVAQLKHSEETWDSLLNRADNAMYEAKSKGRDGWAVAE"
     gene            complement(7156..8934)
                     /gene="mutL"
                     /locus_tag="PL000_08430"
     CDS             complement(7156..8934)
                     /gene="mutL"
                     /locus_tag="PL000_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNC88965.1"
                     /translation="MPIRLLSSEVASQIAAGEVVERPASVVKELTENALDAGAKSISI
                     SIEDAGRTLVEIADDGHGIPANELELAAARHATSKLVQSEDLFHIQTLGFRGEALASI
                     GSVSHMTITSRVADAAEGARLKVDGGIAGKVEKVGAPVGTTLRVENLFYNVPARLKFL
                     KTDVTERRAIDALVTRYALAYPNVRFKVTDGKQVTLQTAGDGDRRSILAALYGVDVAK
                     QMLEVMAQDDGLSLMGFISPTSLTRSNRKEITFFVNGRWVQDYSLTTALLQAYHTLLM
                     VGRYPLTTLFLEVDPEDVDVNVHPTKAEVRFRSQDKVFSFIQRSARKALLAYTPVASV
                     SPQLWGGSKPASSEGREMGIDWSIGHDDELQVTGSKLQVENETSSSTQPANLPPSNIQ
                     HATFNTVPLLRLIGQIGSTYIVAEGPDGLYLIDQHAAHERVLFEKLMAQHDKKTIPSQ
                     ALLTPEIVTLPPQSAKALMPQLPFLNHFGFEVEEFGANTFQVRAMPALFAGGDPASAL
                     RALVEDFEEDEAPLQSEVEAKLAARVCKRLAVKGGQTLTTEEQRALLNDLEACASPRT
                     CPHGRPTMIHLSVDALERQFGRRGAR"
     gene            8991..9626
                     /locus_tag="PL000_08435"
     CDS             8991..9626
                     /locus_tag="PL000_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HNC88966.1"
                     /translation="MKFTTIFFDLDDTLYPSTTNLWLAIKQRMNEFMRERMSIPEDEI
                     PALREKYYMQYGTTLKGLEKHHDINVADFLAYVHDLPLGNYLTPNPIQREVIASLPTR
                     NLIFTNADIPHAERVLTALNLRDLFSDIVDVTTVAPYCKPMPESFDIAMKIAGESDPS
                     RCVMIDDLRRTTRAARERGMFSILCDGTWSVDDADAYFTDWQDLKNILEAQ"
     gene            9626..9850
                     /locus_tag="PL000_08440"
     CDS             9626..9850
                     /locus_tag="PL000_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88967.1"
                     /translation="MESKTIAALLFIVVIVGINFVMYGIVRGFMTSGQKGRSMLETLS
                     KSLAPNVKNKDEEFQELRKKIEELEKGGQE"
     gene            9964..11295
                     /locus_tag="PL000_08445"
     CDS             9964..11295
                     /locus_tag="PL000_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC88968.1"
                     /translation="MNNDNTLKYVFAALAVIVLVLGAFAGGFVTGQIAQMAGLPSLPG
                     FEAPVVASAPTASPEQQNATPSEVQTLFAPFWEAWNIIHENYVDQPVDDLALMRGAIN
                     GMMQALGDKHSTYMDPSTYEQANSSLQGEYEGIGAYVDTTTKYLTITSPMPGSPAERA
                     GLEPGDQVVAIDGEDMTDTIAEVARNKILGPAGTTVHLTILREGAEGLLEFDVVREKI
                     TIKSATGKMLENDIAYVQITTFGSKTTSELLETLTDLMAQNPKGIILDLRNNGGGYLQ
                     TSVEVISQFVGDGVALYEQYGDGKRTTYDVLPGGLATDTNIPMVVLINEGSASASEIV
                     AGALQDYGRAKLVGVVSYGKGSVQNWIPLSNDNGAVRITIAKWLTPKEHTIHEIGLTP
                     DVFVEMTAEDYKAKLDPQLDAAIETLLAMINGTPIPTSMPTAVPSAVPTQP"
     gene            11368..12060
                     /locus_tag="PL000_08450"
     CDS             11368..12060
                     /locus_tag="PL000_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNC88969.1"
                     /translation="MFYFNPTYLIYMLPAFILMALTSWYVRSAYSKWSRVPVSSRLSG
                     AQAAQRLMSTGNLYGLQIRGVGGELTDHYDPRDKTLYLSQGVGNGNSVASVAIAAHEL
                     GHALQDAEEYFPLRFRSALVPLVNIGSNLGWILIMIGLFLNFTQLAWLGVIIFAGGAV
                     FALATLPVEFDASARAKRLLAETGIIQTEEEKRGVNAVLNAAALTYVAGLVTAILQLL
                     YYVSLVGGRRRS"
     gene            12151..13155
                     /locus_tag="PL000_08455"
     CDS             12151..13155
                     /locus_tag="PL000_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC88970.1"
                     /translation="MKKLFLVVVLFLGIAVVVLSFGELETILFTLRKAHLGYFLLAIL
                     IQMAWLLTTGRMYKSVYRLLGLDESILALSRIAAAANFINIVAPTAGMGGVALFAAEA
                     RRRGHPAGRVTVAAALFLSLDHAAFLCVLALGLLVLVRRNDLDASEISASLFMLGIAV
                     SYAFILYLGYRSEQRLGNLLAKMARFINGIVRPFLKREYLSEVRAREFAHEVAEGFSG
                     LTGRPAVLVRPILWGLLDKILLMCILVCSFLSFEVPFSTGTIIAGFSIAYLFLIVSPT
                     PSGIGVVEGLMPVALSSLNVNWSQAVVITIAYRTVTFWVPFGLGAWAFRSLHSAGEEP
                     "
     gene            13156..15693
                     /locus_tag="PL000_08460"
     CDS             13156..15693
                     /locus_tag="PL000_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC88971.1"
                     /translation="MNPLNTIQPLLDFWKRDEQTAPNFFAWQTSPPRPAQTHPIPTDV
                     PAPLREALSTRGIDQLYSHQLSAWTWSRAGKHIILSTGTASGKTLAYNLPVLAAMLQN
                     PEARALYLFPTKALAQDQLSTLANLKPETWNISPAIYDGDTPQANRPAIRKTARIVLS
                     NPDMLHTGILPHHANWSDFFSNLKFIVIDEGHTYRGVFGSHVANVLRRLKRVANFYGA
                     RPQFILASATIGNPKELAERLIEEEVELIDNDGSSRGERHFIIYNPPITDAALGLRKS
                     SLLESVRLATDLLNHNIQSVVFARTRRSVEIILTYLQSPIANLQSSTVNQVRGYRSGY
                     LPHQRREIEQGLRDGSVKTVVATNALELGIDIGGLGAAILVGYPGSIASAWQQSGRAG
                     RGDDPAVSVLVASPNPLDQFLAHHPEYFFGRSPERALVNPNHLLILLEHLRCALFELP
                     FQQGEGFGSISDELLDEYLRFLLANGEVHLSNEKYYWMKDQYPAANISLRSASPQGVV
                     LQSITEDGRPLTIGTVDGESAAWMVHPGAIYMHEAQQYFVQEYNIENRIAQLVPVGLD
                     YYTEAQKNSEIEVLGEAEQAIVPGGVKAFGEIQVTTQVVGFRKIRWFTYENLGQEPLD
                     MPPSELQTTGYWLSLSEEAVNSLRDAGVWTNDPNDYGPDWQKIRLAVRKRDQFKCQVC
                     GAVETVREHDVHHKIPFRSFVREGRVDREQANRLENLTTLCPSCHKKVEQNVRIRSGL
                     AGLGFVLGNLAPLFLMCDPRDLGTHTDPAWSAVNGQPSVVLYDLVPAGIGFSQKLFEI
                     HDELMQRACELVEQCACKDGCPSCVGPGGENGTGGKAETLAILKHLV"
     gene            complement(15820..16230)
                     /locus_tag="PL000_08465"
     CDS             complement(15820..16230)
                     /locus_tag="PL000_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88972.1"
                     /translation="MKITRDVITDLLPVYLSGEASQDTKALVDAFLKDDPEFSKLVRE
                     NGAILPESQINLSKENEMDTLLKTQKLLRQRSTYMAFTILFTLLPLSFYIDAQGMHWM
                     WTDTPINAAIFAGFAIIFGFQYWRISQKLKGSDL"
     gene            complement(16227..16721)
                     /locus_tag="PL000_08470"
     CDS             complement(16227..16721)
                     /locus_tag="PL000_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC88973.1"
                     /translation="MVVNFHELYKKYAQDVHRFAYWLCGNEQEAEDITSETFIRALTA
                     AENIRAETVKGYLLTIARNLALKKLKQAKRITDLTTNLPAAFEGLDRTVETRLSLQGV
                     MQFLQSLPEVDRAALLMHIQEDLPYEEIARALGISVASARVKVHRMRIKLNEYRMKAE
                     ESAP"
     gene            complement(16805..17188)
                     /gene="ssb"
                     /locus_tag="PL000_08475"
     CDS             complement(16805..17188)
                     /gene="ssb"
                     /locus_tag="PL000_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC88974.1"
                     /translation="MPTLNRVQLIGRLGRDPESKFTPTGKKVTTFSVAISNRWKDRNG
                     EMKESTEWVNIEAWERLGEVCQEYLHKGSLVFLEGRLKTEKYEEKGETRYYTKVVLQS
                     LEFLDKRSAEEPMMAVEEDPGDYEA"
     gene            complement(17294..17509)
                     /locus_tag="PL000_08480"
     CDS             complement(17294..17509)
                     /locus_tag="PL000_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88975.1"
                     /translation="MAYSHTNSKGTTYFLHQKGKMFFFSKEVKEGALDALPAGYTVSE
                     MKTGMLVLKRIVAEGEAKPAETDQPAS"
     gene            17604..18437
                     /gene="panC"
                     /locus_tag="PL000_08485"
     CDS             17604..18437
                     /gene="panC"
                     /locus_tag="PL000_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC88976.1"
                     /translation="MRTIISLDELKAARLSFNGTVGLVPTMGYLHEGHLSLIRRAKAE
                     CDHVVVSIFVNPTQFGANEDLAKYPRDLERDLSLIEPFGVDLVWTPTPEIMYPTGYQT
                     WVEVEAMTRPLEGAMRPTHFKGVTTVVAKLFNATQAHKAYFGQKDAQQVAVIRQMVWD
                     LNFPLEVVVCSTVREADGLAMSSRNKYLEGESRQAATILFRALTAAKEMYQTGERDAE
                     VLREKMKEVLGTEPLAQVQYVSCADYNTLEELDVVKGKTLLSMAVLIGKTRLIDNFVL
                     G"
     gene            18440..19357
                     /locus_tag="PL000_08490"
     CDS             18440..19357
                     /locus_tag="PL000_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88977.1"
                     /translation="MDLRQQLGFVLKISGRVLLLAGISLLVLGGMGVYDVLHPPRIVP
                     PGYTLVKENIDFRSVDLLTEDGIRLSAWYTPPDDERKGVVILLAHGYGDNRPEWVYAM
                     IAKHGYGVLAWDARAHGESEGTISTIGYREVLDVKAALDFALAQPGVDHVGAWGGSMG
                     GATLIRATASLPQIEALVVDSSFSSLGEEVDFLAPYPFVNPLAKWMLTFVLDHDLYSA
                     SPLEKISKISPRPVYIIQGSADKVASPDSAFKLYNAAQDPRYLWVEEGARHLGTFQEN
                     PRRYERRVIRFFDEAFFGELARLFEQKQP"
     gene            19354..20235
                     /locus_tag="PL000_08495"
     CDS             19354..20235
                     /locus_tag="PL000_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC88978.1"
                     /translation="MKNRRYWRNLVLMLAFSLLLAFFIFLVFVSDQWAQARLHPARVI
                     PNDDLLVENGIPFQEIELNTKDGVTLSAWYTPPKNGAVILVAHGYNSSREAAWHLLFA
                     KHGYGVLSFDFRAHGLSGGEVSTLGYFEQADVEAALEYALSQPEVEHVGAWGGSMGAV
                     TVLLAAADRVEIEAVVSDSAFPTLEDVLAINVPFGLMKPFVMFFGEYRSGVDMDLVRP
                     VDDIARIGPRPVFLIDGWEGGTIIMNSPYRLYDAAREPKQIWVEDGVPHLAMHAYFPE
                     DYENRVVGFFDEYLLAH"
     gene            complement(20301..21530)
                     /locus_tag="PL000_08500"
     CDS             complement(20301..21530)
                     /locus_tag="PL000_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88979.1"
                     /translation="MQKNIRFYSALLALFVVALACANPLGGTSPVEPAGVETAVAATF
                     QALTAPAAESGDAPPASTSDLLPHSIYFLNTDGAGLTQVHRLEKDGKTVTQITFEPAK
                     VEDYAVSPVDGSVVYTSNNQLLNVLADGSNRSLLVDGGKVDENNRYLTYISNPVFSPN
                     GQTIAFGYKGLSFYSIVSGQYNTVLANTFETLDGGYSFPLELFWPETYSADGSKLLIT
                     LGYMEGASAAVYYPNGGALVRLTGGEGAMICCGETEWTSDGSAFYAASPTFGMFSAGL
                     WRVDAATGAVTTLLNGSFDTNPLNLADEPFLAPDGQLYFFHTTMPGGTDMVNRAALQL
                     VRAGADGATNRTVLRPETFERMNEALWAPDASFVIVANADLPEIHQGGRLELYYTDGQ
                     KGMISLLPFGMDMKWGP"
     gene            complement(21597..21857)
                     /locus_tag="PL000_08505"
     CDS             complement(21597..21857)
                     /locus_tag="PL000_08505"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNC88980.1"
                     /translation="MKLIIIIVKDNDADTLTQAFTAGNFRVTRVASTGGFMRSGVVTM
                     LLGVEDPQVDAAIQVVRDSLPSKGDQKRATLFVVPVQHFEQV"
     gene            complement(21910..22245)
                     /locus_tag="PL000_08510"
     CDS             complement(21910..22245)
                     /locus_tag="PL000_08510"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HNC88981.1"
                     /translation="MENPIHLLLVAVVQDQDLDSATKAVQAIGAAVTYLSSSGGFLGR
                     RNATLLIGLPAEKEADALAALQEACRQRIEYMTLPLEGSPMPMPAPVPVTVGGATVFA
                     LPVERFEQI"
     gene            complement(22367..23653)
                     /locus_tag="PL000_08515"
     CDS             complement(22367..23653)
                     /locus_tag="PL000_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HNC88982.1"
                     /translation="MVFPAILSTVIFLGSLILIFWEKINRSIIAIAGSVLMIGIGKVM
                     GFYSEEAAIEAIDFNTLGLLLGMMMLVAMLEPTGFFEYLAVLAARTSKGKPTQLFFLL
                     GSITTVLSMFLDNVTTVVLIAPVTILISEILGVSALPYLVTEALLSNTGGVATLVGDP
                     PNVLIGSAAGLSFNDFLVYSLPIVLVSWAGALFLLKHLFKKELSKRPRRSKAVMKLNP
                     AESFNQPETARKILIVLGGAILLFFVHHLLHIEPSFVALSAAAVALLWVQPDIHEILK
                     KVEWSVLIFFGALFVMVGGLEHAGTLEMAVGLFENASGWSPVLFGVLIIWIVAALSAV
                     VDNVPITIALIPVIKGLGETGIDITPLWWALAFGAGFGGSGTIIGSSANIIVATLSEK
                     TRTPITASLWIKRGLPVMLVTCAIASILYVLIFPWM"
     gene            complement(23653..24114)
                     /locus_tag="PL000_08520"
     CDS             complement(23653..24114)
                     /locus_tag="PL000_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC88983.1"
                     /translation="MNVTLCMKRQVVSIPVTTTIREAAAIFTKKHIGLLPIVDKNNKP
                     VGVIGIRDLLKLEMPDFINFVVDVDFVHDFGAVEDTRPSAKTLNKSVKSLMRPVLTVE
                     EDCGLLRAYALMLQQNLHDIPVVSKDGQLIGIASRVDIGSTILSTWARVEK"
     gene            complement(24185..25270)
                     /locus_tag="PL000_08525"
     CDS             complement(24185..25270)
                     /locus_tag="PL000_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNC88984.1"
                     /translation="MTNSFLLFLAGLILLILGADLLVRGASRLAAAFGISPLVIGLTI
                     VAIGTASPEIAVSLQAAANGQVDLTLGNVLGSNIFNILFILGVTSIVAPIAIAGQLIR
                     KDAPIMLVVSLFVLALSLDGNIGAIDGGLLLLLLAAYTAFALKQSRIESSEVQNEYNK
                     EFAKKEPRTAKGIVINFVSIAIGLGLLILGSNWLVDSAVAIAKSLGVSELVIGLTIVA
                     VGTSLPEVMTSVIAALKNESDIAVGNAVGSNIFNLLGVLGAGAFFSPNGITVAEQVLQ
                     FDFPVMVFAALVTLPIFYIDNRISQLEGALLLGYYSIYLGYIVLQATQSSFLPLFSLF
                     AAFFTPATFVGLVIIAIRTSGLMKKIS"
     gene            complement(25402..27201)
                     /locus_tag="PL000_08530"
     CDS             complement(25402..27201)
                     /locus_tag="PL000_08530"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HNC88985.1"
                     /translation="MFKHSEFTPIHNIIVPFIHEGPGLHALDAARQFDAEITLVGVVV
                     VPQDQSLSVGASAARALRRLLRVFGRDKRTTSKSQVIVSHEPWNELAKLLQDEKPDLL
                     CLEYESHFKAMCVTPSDALSRPPCDIALVRGKISSKPKQVLVPVRGGPHAELALRVGL
                     GLHVQGVTALHLRRTNDPEAGTDAPFKGLERVLKQMPEVQKQFEVTDDSAQFILDHAR
                     QKDVIILGTTIQPLTSSVSLGPVADRILREAPCAVIAVKSGRPIPHAVYDESAGVEAI
                     SILVDKWFAENTFHADEFNQLRELIELKKKQGLSISLALPALNEEETVGRVIKMMKKE
                     LLQRIPLLDEIVLIDSDSTDRTREIAKKEGVPVYIHQQLLGRLGARRGKGEALWKSLL
                     VTKGDIIVWIDTDIVNIHPRFVYGIIGPLLTNPQVQLVKGFYRRPLRVGQKMQAGGGG
                     RVTELTARPLLNLFYPELSGVVQPLSGEYAGRREALERAPFYSGYGVETGLLIDIYER
                     YGLNAIAQVDLLERIHHNQSLEALSKMSFAIIQTVMRKLENRVGRVMLEDVNRSMKLI
                     RYNNKGYSLDVEQIAELERPPMIEVQEYLERQK"
     gene            complement(27217..28431)
                     /locus_tag="PL000_08535"
     CDS             complement(27217..28431)
                     /locus_tag="PL000_08535"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC88986.1"
                     /translation="MNIAILHYSVPPIVGGVESVIGHHARLMSADGHSVRLIAARGES
                     LSDQILLTEIPLADSRHERVTHMKNQLDHGEVTQDFESLRDELVFQLQTALADVDILI
                     AHNVCSLNKNLALTAALYQLHVSKKLPRLILWHHDLAWTTPRYQPELHDGYPWDLLRT
                     DWGGTTHVVVSHLRRRELSTLMKIPPDEIQIIPNGVDAARFYKLESQTQSLLEKTKLL
                     DATPILLLPVRVTPRKNIELALHTLAELRKRFADAALVVTGPLGPHNANNQKYFESLS
                     SLRTQLGLQHSAHFLSELTDSFLPDEVIADFYRVADALLFPSREEGFGIPLIEAAFSH
                     LPAFCADIPPLRELGLNDAAFFSPDEDPVKISAMIAEYFQSSPAARLSMRARAAFRWE
                     AIYRQHIAPLLK"
     gene            complement(28428..29771)
                     /locus_tag="PL000_08540"
     CDS             complement(28428..29771)
                     /locus_tag="PL000_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC88987.1"
                     /translation="MTNPSHHIGFISTRFAGTDGVSLETQKWATVLERLGHRCFYFAG
                     ECDRSSDQSQVTPEAFYRHPEIEKINQQAYSGSWTVTQKARSEHPEIAALQKDFFSIY
                     VRPPQMTRRVHELKEHIKAELYKFARRFDLEILVVENAVTIPLNLPLGLAITEFIAET
                     GYPTIAHHHDFHWERQRFQVNCVGDYLAAAFPPTLPSIRHVVINSLQAQQIATRYGVT
                     SRVIPNVMDFDSPPPAPDAYTQNIRADFGIEQGEYFFLQPTRVIQRKGIEHAVELVRR
                     LGVPAKLVISHASGDEGSDYEHRVREYAHLLDVTVRFEADQVQDERGTTKDGRKIYTL
                     GDVYPHADLITYPSSIEGFGNAFLEAVYYRRPILVNNYSIYEVDIKPKGFRAVWFDGF
                     INVETLKKVREILLNPAEAQAWAEENYQLAKRYFSFTVLERRLQSILADCLGQQV"
     gene            complement(29799..31490)
                     /locus_tag="PL000_08545"
     CDS             complement(29799..31490)
                     /locus_tag="PL000_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HNC88988.1"
                     /translation="MTITLLDHLTFLYGENDSTRLLELTHSLLNNYRTRIEPRDGKST
                     ERDSILITYGDQIQTPTERPLQTLRKFCKTHLTDIVSGIHILPFYPWTSDDGFSVVDY
                     RQTDPSLGNWDDVSSMQSFRLMFDGVINHISSQSAWFQGFLRDDPHYRDYFITVEGDP
                     DLSKVVRPRALPLLTTFKTAAGEKKVWTTFSEDQIDLNFKNPEVLLEILDILLIYAER
                     GASFIRLDAIAYLWKEISTTCIHLPQTHHVIQFLRAALNEVAPHIQLITETNVPHADN
                     ISYFGDGTNEAQLVYNFALPPLTLHTFHTGNAGKLSNWARTLALPTDKTTFFNFLASH
                     DGIGINPVRSILSNEEINALVHKTLEHGGLISYKHNTDGTQSPYEMNINYFDALSDPN
                     AHEPIDLQVNRFIASQAIMLALIGIPGIYFHSLFGSRGWFEGVRQTGRNRTINREKLQ
                     LDELQNELTDEHSLRSKVFTKYSQLLKARSSSSAFHPHGAQTVLDSHPSIFAIERLSP
                     DGASRVLCLHNVCDQIISVSTGHKSAIDLFTGQAIQASDVRMLPYQVQWLKINQV"
     gene            31649..32299
                     /locus_tag="PL000_08550"
     CDS             31649..32299
                     /locus_tag="PL000_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC88989.1"
                     /translation="MPDDKNMISIILADDHQMARQGIRSILEKAPDFEIVGEAKDGDE
                     VRRLVAALRPQILLLDLKMPNLSPAELEKWVRINYPETNTLVLTGHDRDVYLANMMDA
                     GVSGYLDKKLRAGQLISAIRRAARGEIFFDEEQIERARRWREEVGNKWASLSEREKEI
                     LQKLTEGAENLAIAEMLCITTNTVEKHLTNIYKKLGVKSRLEAAIWWLEKSGDFRN"
     gene            32556..33173
                     /locus_tag="PL000_08555"
     CDS             32556..33173
                     /locus_tag="PL000_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88990.1"
                     /translation="MKPLFKITSGFSLDLVMILIVAGILVSCSQKSVVVGSTRNQQLP
                     IVVRSTEVTSIEQHKGFETTYGIIVAVKALCKDDVNTILYLKTNFDASLWHLNEKEFY
                     PFGGAYFETSILFLENGEMFSMYASGKRDEPIFDLQNNMVSTMQTFVFPKIPSEESTF
                     TIKAKVSLHDLPVTYIPPIKVDFLEPGIIEIPMEYIVSAEIGECP"
     gene            33262..33993
                     /locus_tag="PL000_08560"
     CDS             33262..33993
                     /locus_tag="PL000_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88991.1"
                     /translation="MNKKNMLVSAFAATFVVVIAGIVAMVVLYFQPKTVIAKSVEPVS
                     NYAESSTVVNVPSLENSNAAEVKYPLAQEINGIKMEVTGTAMEGEYFTTDVCYDLPAP
                     KTGYEFTLGGQTQKSIALTNAKNETIMIYSWKIIGGFNQDANGNFQGNCARLYFPVLS
                     DTNLDGLTLTISRMSTPVADFPDCEKAQQKLDEAKQKIKVECYENGGFGVSEKPDDMS
                     DQEAREIALEAFIEIVEGPWVFKLK"
BASE COUNT         8215 a         8445 c         8766 g         8686 t
ORIGIN      
        1 ggatggacga gtgtttgatc agggtgtcat gtgagccagt gtcaggtgtc aggtttgaag
       61 gtttaaaagt ttgaaggttg gcaggttgat tcgccgcttt cgactttcca ctttccacat
      121 aattcagtac atcgtttttg gtaatacgtc cgttcagccc tgtgccattg acctgtgaca
      181 agtccacgcc tttctcggcg gcgatttttg cgacgacggg ggagatgaag cccaggttgt
      241 tggcagtgga tggtgtgtag ttggtagttg gtggttggtg gttggtggtt ggtttaacag
      301 gagtgttgct atcaactgtc aactgtccac tgttaaccat caacagtcca ctgtccaccg
      361 attctccggg ctgaccaatg atcgcgagca attctcccac tttggcgggg gtgccttcct
      421 gtgcgatgat ctgcagaatt gttcccgtca cgggcgcggg gatttcggta tccaccttgt
      481 cagtgttgac ttcgagtagc ggctccaact cattgatcga gtcgccgacc tgcttgagcc
      541 attttgtcac ggtcacttcg tccacgcctt cgccgagtct gggtacgagt actttggttg
      601 ccattgggtt tctccagttg gtagtttaca gttggtagtt gatggttaac ggtttgtggc
      661 tgatggtagg ctattgataa gcgcgtaaag ctttttacgc aggttttgaa tttccatatc
      721 catggggtca atatcttctt tgctcaaata tccaaattct tgtgccagta tcaattgtgt
      781 gtccacttcc gccagggagc caagcgcaat gtgtaagaat cttcgaaagt ctccaggtga
      841 tttccttgcc tgaccttctg caatatttga tggaacagaa actgcggcgc ggcgaagttg
      901 atctgcgaga gcataggttt cctgcttggg gaattttcca gttagtaagt aaactgcctt
      961 ggcaatcttg attgcatcct gccagacaat caactcacga taattctttg cagaaatttt
     1021 ttctttttcc ataaagcctc actatcaacc accaactccc aaccaccaac catgttcaca
     1081 tcaactaaac agcgggtacg cccctggatc aacctcattc atcatctcat acaccgcatc
     1141 gaagatcgtc tcggcattcg gcttggacca gtaatcgccg tcgcttccat acgcggggcg
     1201 atgggctttg gcagataagg tgcgggcggg ggagtctaaa tggaagtagc cgccttgttt
     1261 ttcgatgact tcctgcatca tgtaggcggt tgtgccgcca gggacatctt catcgacaaa
     1321 caaaatacgg ttggtctttt tcagcgattc aacgatcttg ccgtggatgt caaagggcat
     1381 cagcgattgc acgtcgatca cttccacatc aatgccgacc ttgctgagtt tgtctgcggc
     1441 gtcgagcacg actctacagc aagcgccgta ggtgacgatg gtgacatctt tcccgtcacg
     1501 aataatttca ggcacgccga gtggcagcgt gatctcgccg atattttcgg gcaggcgttc
     1561 tttgacgcgg tagccattga gcacttccac cacaatggcg ggttcgtcag atttcaagag
     1621 ggtgttgtaa aagccagcgg cgtgagtcat gtcgcgcgga acaagcacat acataccgcg
     1681 agttagattc aaaatgccag acatcggtga gcctgaatgc cagattcctt cgaggcggtg
     1741 accgcgagta cgcacgatga cgggagcttt ttgcccgcca gcggtgcgcc agtgcagggt
     1801 ggcaaggtca tcgctcatgg tttgcagggc gtacagcaca taatcaaggt attgaatttc
     1861 ggcaatcggt cgcagtccac gcattgccat gccgattgcc tgccccatga tcgtcgcttc
     1921 gcggatgcct gtatcggtca cacggaactc gccgtatttt tcctgcatcc ctttgaagcc
     1981 ctgattcaca tcgccgagtt tgcccacatc ttcaccaaag gcaatgatgt gcgggtctct
     2041 tgtcagcgcg gcatcgaagg cggcattcat gatctcgaag ccgaacatgg tcggcgatga
     2101 ttcagaatac tcaggcttga cctcgctcac tttcatcgct gtgccagaat acaaatgcga
     2161 accgtaacgc tccacgttca cgctgtcgtt ctcatgcttc cattggatga ggacctgctt
     2221 cgatggatgc gattcgtccc gcagcaatct cagcgcttca tgcacgatcg aatggatgtc
     2281 acgcctcgaa tacgacggga gagtcgccag ccgatcccga atcagtctca actcgtagac
     2341 atggttcgag gtgggcgcga tctcgtcgag catgtccaat acttggttgc gctcttcggt
     2401 gatgggggcg aggtaggcat cccatgcggc tttgcggaat ccttccacgg tggcgtaatc
     2461 ttccttttca acagaatcaa gctcaggcgc agagatgact cggtttgtga tcatccaagc
     2521 gcgcatcttt ttgagtccgt caaattcttc ctcccatttg agtcgctctg gtgttttgta
     2581 gcgctcatgg ctgcccgatg tggaatgtcc ctgtggctgg gtcacatcaa tgacatgcac
     2641 gagcgcaggg atgtgatatt cgcgggcgat ctcagcggcg gtgaggtagg tttcgacgag
     2701 ggcggtgtaa tcccacgcgc ggacggtgta atggtcgtat ccgttttgca ctttgtcggg
     2761 tggcgtgaat gggtcgcgct caaagccctt gagcagcgca ccgatatttt ccttgaccat
     2821 ttgaaattga ttggggacgg agattccgta gccatcatcg tacacggtga tgatggcggg
     2881 cgctttcaat acgccaatgg cattgactgc ttcccagaac aagccttcag atgtggatgc
     2941 attgccgatg gtggcaaagg cgatctcgtt gccgttgatc gaaaaatctt tttgatcttg
     3001 cagttctttg atgttgcgat acatgaccga ggcatacgca aaccctacgg ctcgcggcat
     3061 ttgcgcggcg gtaggggaaa tatctgacgc ggtgttgtac atctgtgttt gcgggcgcca
     3121 tgtgccgttt ggataaagat tgcgcgatgc gaagtgggcg ttcatttgcc gccccgcgct
     3181 ggcggggtcg taggtgacat cgccgtgcgc gtacagctgc gcaaaaaatt cctggatgct
     3241 catcacgcca agcatgaaca tccaggtttg atcgcgataa tatccagaac gccagtcgcc
     3301 cttttggaag gcgcgggcaa tggcaagctg ggcgatctct tttccatcgc cgaaaatgcc
     3361 gaactttgcc ttgccgctca aaacttcacg cctgccgata attgaagcct gcctgctttg
     3421 ataggcaagg cggtaatctt tgatgatttc atctttttct aggggaagcg caatcgtacc
     3481 cttctttttg ttgggcatga actctctctc ctgtaaagtt tgttgggcgg attataaacg
     3541 ggaacggatg aagaaagaag aaggaagaaa gaaaagggcg gacatttctg cccgcccgct
     3601 gtctctggtt acttttcaac ctacccccag ccactccatt gctttttcca tctcgctgaa
     3661 tatgcgggca ttcaaaccgc gattgacgct gacagtttca gcgaaccagt cgatttcctc
     3721 cgcgcggtat agaacggcga tcttcacctt gttgcggaag attaaagcgc cctgcacccc
     3781 gatctcgaaa cgctccaacg cttttacctt ccccgtcatt tcggatacat taaccagtac
     3841 tttttgataa tgctcggcct tgcaggtttc gaatacttgt ttcgagatgg taatgaagtt
     3901 ctcaaatgta tatggttcgg catattcaag atagagatat ccattcctgc gttcaatgct
     3961 tttcatgctc gaccctcggt tttggggtaa atataaacgg agatgatttt tcaggtcatc
     4021 tccgtttgcg gttacttttt actttaatct ttctgcgacg atctccgcca catccttgac
     4081 ctgtatttct ccaccatcgc cttttgctgc gtcggtcatc atggtgaggc agaatgggca
     4141 tcctactgcc acagtgcttg cgcctgtcgc ttttgcttcg ttgaagcgcg ttatattcac
     4201 acgcgctgag ccttgttcct cctctttcca catctgcgca ccgcctgcac cgcaacagaa
     4261 cgatttggct gaattgcgcg gcatttccac tgtttcaacg cctgctgatc tcaatgtgtc
     4321 gcgcggcgca tcagtgacct tgttgtgccg ccccaaatag cacgggtcat ggaaggtgat
     4381 cttcatgttg tcgccttcaa ggttcatcga gatcttccct gaaccgacca attcattgat
     4441 caattgtgtg tgatggatga cttggtaatt tccgccaaag gcagggtact cgttcttaat
     4501 ggtgtgcaaa caatgcggac aggttgtcac gatgcgtttc ggcgcaacct cattcaatat
     4561 ctcaacgttt tgagaggcca gcccgaagaa aatatcttca cggcctgcac gcctggcaga
     4621 atcacctgtg caggattcat tctttccaag tacagcatag ttgacacccg ctgtattaag
     4681 gatttttgca aaggcttgtg cagttttttg tgcgcggctg tctgttgcag gggcgcagcc
     4741 cacccaccat aaaatttcgg gctcggcatt ttgctcaatg gtgggcacat tcatgccctc
     4801 ggcccatttc atgcggtcgc cttgcgaaac attccacgga ttctggttgc gctccatgcc
     4861 tttgaatgcg gtctccaatt gtttggggaa ggcgctttcc atcatggata ggttgcggcg
     4921 gatatcgaga atgtcgcgca tcggttcatt gcccacaggg caaatgtcca cgcaggcgcc
     4981 gcatgaggtg catgcccaga ccgcttcttc gctgataagt tcggtcatgg cgacatcggt
     5041 cgtgccgccg ccgttgaagt gatagcgttt gttgatctcc aacgcggcag gggagagcaa
     5101 cttgcccgta ttgtatgcgg ggcaaacttc ctgacagcgg aagcacatga tacaggcgta
     5161 gctgtccatg atctgctccc agccgaggtc tttcatcttt gccgcgccga attgctcaat
     5221 ggattgatcg tcgaggttga tgtaacttaa ttcaccgatc gattttcttt caggcttcaa
     5281 agcaaagttc aaaggcgcga agaacaggtg aatgtgtttt gaatacggga aataaggcag
     5341 gaatgcgacg accgcaccaa tggacagcca gaatgcgata tgctcgcctg ccaccaaaac
     5401 actcgcatcc acccctgacc ataaacctgc cgctgctgag atgcttggtt gccatctgtc
     5461 agagtgacct gccaacgcca ccccaaacga ttctcccaaa aagcgcatgg tgttgtggac
     5521 gaagatgaac gtggcaacga tcgcagaatc acgcatgatg ccagtccgtg ctttcgggtt
     5581 gagcaatgtc gtctcacgtg tggacagagt cgcaggtcga aggatgaagc ggcggaacgc
     5641 catcgccaga atgccgacga tgatggcgac gtttgcaatg tccgcgagca ggcggtaggc
     5701 atcgccaaaa attcccagat gttccagtaa tttgtaattg gtaaatgcgt agatcagatc
     5761 cgcgaggttg atgaggagga aggaaagaaa accccagccg ataagcgcat gcagaatgct
     5821 gggtccgagc ctgaaacgaa aaacgggttg gaacagcccc accttgacga tgaggtcacc
     5881 aatccgtttc cataagagtg accaatctgc cttgccctgt ccgcttgaga tatgcccgac
     5941 aattttcatc acacctttgt aggtgaagta caacgaaacg agtgttgcaa agacaaaaag
     6001 gattttttct gcaagggtca gcatggaaac tccaaaatat aagaaatgtt cgttaagttt
     6061 agcacaaatg gggagataaa aaaatagccg cgcgaggcgg cttggtttcg tttgttattc
     6121 tgccacggcc cagccgtctc gtcccttgct cttggcttcg tacatggcgt tatcggcgcg
     6181 gttgagcagg gaatcccagg tttcttcact gtgttttagc tgtgccacac cgatactgac
     6241 cgtcacattc agtacctctc cattattgat cacgatcttc atctctcgga ccaactgaca
     6301 caaccgtgat gcctgttctt cagcagcttt ttgacctgaa ttcggcagca ggatgaggaa
     6361 ttcttcgccg ccgtaacgac cgactacatc agggtggcgt acatggtcac gtaaatggat
     6421 tgcgatttga cggagcactt catcacctgt catgtgtccg tgggtatcat tgatggtttt
     6481 gaaatgatcc acatccacga tcgagatcga aaattgattc tgatatcttt ctgcgcggat
     6541 gaattcatcc tgtaattcac ggatgatggt gcggcggttt ggcagaaaag tcagtgcatc
     6601 ggtttcggcc acttcacgcg cctgagccat cactccttcg atctccacct gctttcggcg
     6661 tgcgagcttt ttgctttgct cactctcgat tttaaataat ttgacctgtc tgttcagccg
     6721 ttccacttcc tgaaatgcgg aatcaaggtg gatttcctcg gcaataaaaa ccatttgtcc
     6781 gtttgccagc ggtaccagat gacaatcgca ttgaatgaca acttcacttc tgcctgcagg
     6841 cagggggagt ttgatgatcc aatgttctct ggctcgtgaa tttaatttcg aaatgatcag
     6901 atctctgtcg tctggtgcga aaaaatcccc caatgttttt gcctgcggaa ataacttttt
     6961 gcatgcttca aatgcgcggt tccatgaaat caacaatcct tctttttcaa ccagtgcgat
     7021 catggcatgg atgttttcaa ggatgatctc tgcatcggtg gctgtgatgg tttccgccag
     7081 ctgtttcata tctccattgt agcccattca ataaatattt atgtccacct tacaaattta
     7141 gcaagcaacc catgattacc tcgcccctct tctcccaaat tgacgttcca gcgcatccac
     7201 cgacaagtgg atcatcgtcg gcctgccatg cggacaggtg cgcggggaag cgcaggcttc
     7261 gaggtcattc aacagcgcgc gctgttcttc ggtggtgaga gtctgcccgc ctttgaccgc
     7321 cagccgtttg caaacccgcg ccgccagttt tgcttccacc tcagattgca ggggcgcttc
     7381 atcttcttcg aaatcttcca ccaatgcccg cagcgcagat gcagggtcac cgcccgcaaa
     7441 caaggcaggc atggcgcgga cttgaaaggt attcgcgccg aattcctcca cctcaaagcc
     7501 gaagtgattc aaaaacggca gttgcggcat cagcgccttt gccgattgcg gaggtagtgt
     7561 cacgatttcg ggcgtgagca aggcctgtga tggaatcgtt tttttatcgt gttgtgccat
     7621 caatttttcg aacaaaaccc gctcatgcgc cgcatgttgg tcgatcaaat acaatccgtc
     7681 tggcccttcc gccacaatgt aggtcgagcc gatctgaccg atgagacgca gtaggggaac
     7741 ggtattgaag gttgcatgtt gaatgtttga agggggcagg ttggcaggtt gcgtgctgga
     7801 cgaagtttcg ttttcaacct gcaacttgga acctgtaacc tgtaactcat catcatgccc
     7861 aatcgaccaa tcgatcccca tctctcggcc ttcagaggac gcgggttttg atccgcccca
     7921 taattgcggg gagaccgaag ccacaggcgt gtaggccagc agggctttac gcgccgagcg
     7981 ttgaataaaa ctgaagacct tgtcctggct tcgaaaacgt acttccgcct ttgtcgggtg
     8041 gacgttcaca tccacatctt ctggatcaac ctccagaaac aatgttgtca gcgggtagcg
     8101 tcccaccatc agcagggtgt gataagcctg caataaggcc gtggtcaatg aataatcctg
     8161 cacccagcgc ccattgacga agaaggtgat ctctttgcgg ttcgagcggg tcagcgacgt
     8221 ggggctgata aagcccatga gagacagccc atcatcctgt gccatcactt caagcatctg
     8281 ctttgccacg tccacaccgt ataacgccgc aaggatcgaa cgcctgtctc catcaccagc
     8341 ggtttgcaat gtcacttgtt ttccgtcggt cactttgaaa cgcacattcg ggtatgccag
     8401 cgcatagcgc gtgaccagcg catcaatggc gcggcgctcg gtcacatcgg ttttcaaaaa
     8461 cttcaaacgt gcgggcacgt tgtaaaaaag attttccacg cgcagggtcg tgcccacggg
     8521 cgcgccaact ttttcaacct tgcctgcaat gccgccgtcc actttcaggc gcgcaccttc
     8581 tgcagcatct gccacccgcg aagtgatggt catgtgcgag acagaaccga tcgaagccag
     8641 cgcctcgcct cggaagccca atgtctggat gtggaacagg tcctcggact gaaccagttt
     8701 cgaggtcgca tgacgcgccg cagcgagttc caactcgttc gcaggaatcc cgtgtccgtc
     8761 atctgcaatt tcaaccagag tccgccctgc atcttcgatc gaaatggaaa tgcttttcgc
     8821 acccgcatcc agcgcgtttt cagttaattc tttaacgacc gaagcggggc gttccaccac
     8881 ttcacccgcc gcaatttgcg aagcgacttc agaggataaa agacgaatag gcatgacgcg
     8941 attataaccg cttgcagttt gcgcaaattg gaggcgggta taatcgccga atgaaattta
     9001 ccaccatctt cttcgacctc gacgataccc tctacccgtc cactacaaat ctgtggctcg
     9061 ccatcaaaca acgcatgaac gaatttatgc gcgaacgcat gagcatccct gaggatgaga
     9121 tccccgcttt gcgcgaaaaa tattacatgc aatatggaac cacattgaaa gggctggaaa
     9181 aacatcacga tatcaatgtt gcggattttc tggcttacgt gcatgacctg cccctcggca
     9241 attacctcac cccaaacccg attcaacgcg aagtcatcgc atcgctcccg acccgcaacc
     9301 tgatcttcac caacgcagac attccccacg ccgaaagagt gctgaccgcc ctcaacctgc
     9361 gcgacctctt ctccgatatt gtcgatgtaa ccaccgtcgc gccgtattgc aaacccatgc
     9421 ccgagtcgtt cgacatcgcc atgaagatcg caggtgaaag tgacccctcg cgctgtgtga
     9481 tgatagacga cctgcgccgc accacccgcg ccgcccgcga acgcggcatg ttcagcatcc
     9541 tttgcgacgg cacctggagc gtggatgacg ccgacgcata tttcaccgac tggcaggacc
     9601 tgaaaaatat tttggaggca caataatgga atcaaagacc atcgccgcgc ttttgttcat
     9661 cgtggtcatc gttggaatca atttcgtcat gtacggcatc gtgcgcggat tcatgacctc
     9721 cgggcagaaa ggccgcagca tgttggaaac cctgagtaaa tcgcttgccc cgaacgtgaa
     9781 aaacaaggat gaggaatttc aggaactgcg caaaaagatc gaagaactgg aaaaaggcgg
     9841 gcaggaataa cttccgttac cgatggggga aaagccctct tacacaaatc gtacaaagat
     9901 ttcagcaaac attaatcccc gagtggtatc tttccactta gttagccaaa tttggaggca
     9961 tctgtgaata atgacaacac tttaaagtat gttttcgctg cattggccgt gatcgtgctc
    10021 gtattgggtg cgtttgcggg tggattcgta accgggcaga tcgcacaaat ggcggggctg
    10081 cccagcctgc cggggtttga ggcgccggtt gttgccagtg cgccgaccgc ttcccccgaa
    10141 cagcaaaacg ccaccccgtc agaggttcaa accctttttg ctccgttctg ggaagcctgg
    10201 aacatcattc atgaaaatta tgtcgatcaa cctgtggatg accttgcatt gatgcgtggt
    10261 gccatcaacg gcatgatgca agctttgggt gacaaacact ccacttatat ggacccatcc
    10321 acctatgaac aggccaattc cagcctgcag ggtgaatatg aaggcattgg cgcctatgtg
    10381 gataccacca ccaaatacct gaccattacc agccccatgc caggctctcc cgccgaacgc
    10441 gccgggcttg aacccggtga ccaggtggtt gccattgatg gcgaagacat gaccgacacc
    10501 attgccgagg tggcgcgcaa taaaattctc ggcccggcag gcacaacggt ccacttgacc
    10561 attttgcgcg agggtgcgga aggcttgctc gaatttgatg tggtacgtga aaagatcacc
    10621 atcaaatcgg ccacaggcaa gatgcttgaa aacgacattg cctatgttca gatcaccacg
    10681 tttggctcaa aaaccacttc agaattgctg gaaacgctca ccgacttgat ggcgcaaaac
    10741 cccaagggaa tcattcttga tttgcgcaat aacggcggcg gatacctgca aacttctgtg
    10801 gaagtgatct cccaattcgt tggggatggc gtggctcttt atgaacaata tggtgacggc
    10861 aaacgcacga cctacgatgt actccccggc ggacttgcca ccgatacgaa cattcccatg
    10921 gttgtattga tcaatgaagg ctccgcctct gcatctgaaa ttgtggcagg cgccttgcag
    10981 gattatggcc gtgcgaaact ggtgggggtg gtgtcttatg gaaagggttc tgtgcaaaac
    11041 tggattccgc ttagcaatga caacggcgct gtgcgcataa ccattgcaaa gtggttgaca
    11101 ccaaaagaac acaccattca cgaaatcggc ctgaccccgg atgtctttgt tgaaatgacc
    11161 gcagaagatt acaaagccaa actcgacccg caattggatg ccgccatcga aaccttgctt
    11221 gcaatgatta acggcacacc gatccccacc tcaatgccca ccgcagtacc cagtgccgtt
    11281 cccacacagc cataatttgg caaagtggtt tcgtactgct ttgaacaact ggtacaactg
    11341 ttttacgtaa taatagcagg agaaaaaatg ttttacttta atccaaccta cttgatatat
    11401 atgcttccag ctttcatctt gatggcgctg acttcatggt atgtccggtc tgcctattca
    11461 aaatggagcc gtgtccccgt cagcagcagg ctttcgggcg cgcaagccgc acaacggtta
    11521 atgtccactg gtaatttgta tggcttgcag attcgtggtg tgggcggaga acttaccgat
    11581 cattacgacc cacgcgataa aaccctgtac ctttcgcaag gcgttggcaa tggaaattcc
    11641 gtggcctcag tggcaattgc cgcacatgaa cttggccatg ccttgcagga tgccgaggag
    11701 tattttccgc ttcgcttccg ctcggcactg gtgccgctcg tcaacattgg ctccaacctc
    11761 ggctggatcc tgatcatgat cggtttgttc ctcaacttca cacagttggc ctggctgggt
    11821 gtgatcatat ttgccggtgg tgccgtcttt gctctggcca ccctgcccgt ggaatttgac
    11881 gcctctgcac gggcaaagag attgctcgct gaaactggca tcatccagac cgaagaagag
    11941 aagcgcggag tcaacgccgt tctgaacgcg gctgccttga cctatgttgc aggccttgta
    12001 accgccatcc tccaattact gtactatgta tcgttggtgg gcggcagacg ccgaagctga
    12061 tcatcttatg taaataaagt gacgaacccc ccttaagtgt gattgggtgg gttcgttttt
    12121 tgttccatcc gaagtgtata atcatttgcg atgaaaaaat tattccttgt tgttgtgctc
    12181 ttcctcggca ttgccgtggt cgtgctgagc tttggcgaac ttgaaaccat cctgtttact
    12241 ttgcgaaagg cccacctggg atattttctt ctggcaattc tcattcaaat ggcctggctg
    12301 cttaccacgg ggcgtatgta taaatcggta taccgcctgc ttgggcttga tgaatccatc
    12361 cttgcactga gccgcattgc cgctgccgcc aatttcatca acatcgtcgc acctactgca
    12421 ggaatgggtg gagtggctct ctttgccgcc gaagcccgca gacgcggcca tcctgcgggc
    12481 cgcgttactg tggcggctgc gctcttcctc tcacttgacc atgctgcttt tctttgtgtg
    12541 ctggcattgg ggttgcttgt gcttgtccgc cgtaacgatc tggatgccag cgaaatttcc
    12601 gcatcacttt tcatgctggg aattgccgtg tcttatgctt tcattttgta ccttggatat
    12661 cgttcggaac aaaggcttgg caacctgctg gccaaaatgg cgcgctttat caatggcatt
    12721 gtcaggccgt tcctcaaacg cgaatacctc agtgaagttc gcgcacgtga atttgcccat
    12781 gaagttgcag aaggtttctc agggttgaca ggcaggccag ccgttctggt cagacccatt
    12841 ctgtgggggc tcctggataa aatcttgctg atgtgcattc tggtctgttc atttctttcc
    12901 tttgaagttc cattttcaac aggtacgatt attgccgggt tttccatcgc atatcttttt
    12961 ctcattgttt cgcccacccc ttccggtatt ggcgttgtgg aaggactcat gccggtggca
    13021 ttgtcttcgc tcaatgtcaa ctggagtcag gcggtcgtca tcaccattgc ctatcgcaca
    13081 gttacatttt gggttccgtt cgggttgggc gcatgggcat tccgctccct gcacagcgca
    13141 ggtgaagaac cataattgaa tcctttgaat accattcaac ctctactcga tttctggaaa
    13201 cgggatgagc aaaccgcccc gaattttttt gcttggcaaa cctcccctcc gcgccccgcg
    13261 caaacgcatc ccatcccgac cgatgtgcct gctccgcttc gagaggcatt atccacccgc
    13321 ggcattgacc aactttactc ccaccaactc tccgcatgga catggtcacg ggctgggaaa
    13381 cacatcatcc tttccacagg cacggccagc ggaaagaccc ttgcctacaa cctgcctgtc
    13441 cttgcggcca tgcttcaaaa ccctgaagcg cgcgcgctgt atctcttccc caccaaagcc
    13501 ctcgcgcagg atcaactatc cacgcttgca aacctgaaac ctgagacctg gaacatatca
    13561 cctgcaattt atgatggtga taccccgcaa gccaaccgtc ctgccatccg caaaacggca
    13621 cgcattgttc tttcgaaccc agatatgctc catacgggga ttcttccgca ccacgcaaat
    13681 tggagcgact ttttctccaa cttgaaattc atcgtcattg atgagggaca cacctatcgt
    13741 ggtgtctttg gctcgcatgt cgccaatgtg ctccgcaggt tgaagcgggt ggcgaatttc
    13801 tacggcgcac gaccgcaatt cattctggct tcggccacca tcggtaaccc gaaagagctg
    13861 gcagagcgtt tgatcgaaga ggaagttgaa ctgatagaca atgacggctc ttcccgcggt
    13921 gagcgtcact ttatcatcta caatccgccc atcaccgatg ccgccctggg gttgagaaaa
    13981 agctccctgc ttgaaagtgt acgcctggcg acagatttac ttaatcacaa catccagtct
    14041 gttgtgtttg caagaacgcg ccgcagtgta gagatcatcc tcacctatct tcaatcgccg
    14101 attgccaacc tccaatcgtc cacggtaaat caagttcggg gatatcgttc agggtatctt
    14161 ccgcaccaac gccgcgagat cgagcaggga ctgcgggatg gctccgtaaa aaccgtcgtg
    14221 gcaaccaatg cgcttgagct gggaattgac attggcgggc ttggcgcggc aatccttgtc
    14281 gggtatccgg gttccatcgc cagcgcatgg cagcaatcgg gtcgggcggg gcggggggat
    14341 gatcctgcgg tatcggtgtt ggtcgcatcc cccaatccgc ttgatcaatt ccttgcacac
    14401 catcccgaat attttttcgg caggtcaccg gaacgggcgt tggtgaatcc gaatcatctg
    14461 ttgattttgt tggagcattt gcgctgcgcc ctttttgaac tgccatttca acaaggtgag
    14521 gggtttggca gcatctctga tgaactgctg gatgaatatc ttcgcttcct gcttgcgaac
    14581 ggtgaggttc acctttccaa cgaaaaatat tattggatga aagaccaata ccctgctgcc
    14641 aatatttcat tgcgttcggc ttcgccgcag ggagtggtat tgcaaagcat aacggaggat
    14701 ggaaggccct tgaccatcgg aaccgtggat ggcgaatcag ccgcctggat ggttcacccg
    14761 ggggcgatct acatgcatga agcccagcaa tattttgtgc aggaatacaa cattgaaaac
    14821 cgtatcgccc aacttgtacc tgtgggtttg gattattaca ccgaggcaca aaagaattca
    14881 gagatcgaag tgttgggcga agcagaacag gcaattgtgc ctggcggcgt gaaagcattt
    14941 ggcgagatcc aagtcactac tcaggtggtt gggtttcgca agatccgctg gtttacgtat
    15001 gaaaatttgg gacaggaacc gctcgatatg ccgccctccg aattacagac cacaggctat
    15061 tggctttcgc tttctgaaga agccgtgaac agcctgcgtg atgccggtgt gtggaccaat
    15121 gaccccaacg attacggccc agattggcaa aagatacgac tcgccgtcag aaaacgggac
    15181 cagttcaaat gtcaggtatg cggcgcggtg gaaactgttc gcgagcatga tgtccatcat
    15241 aaaatcccat ttcgctcgtt tgttcgcgag ggccgtgttg atcgtgagca ggccaaccgt
    15301 ttggaaaacc tcaccacact ttgcccaagc tgccataaaa aagtggagca aaatgtccgc
    15361 atccgcagcg ggttggccgg gctcgggttt gtattgggca accttgcacc gctcttcctt
    15421 atgtgcgatc cgcgcgatct gggtactcac accgaccccg catggtctgc ggtgaacgga
    15481 cagccctctg ttgtcctcta cgaccttgtt cctgcgggca ttggcttcag ccagaaatta
    15541 tttgaaatac atgatgaatt aatgcagcgt gcctgtgagt tggtggagca gtgtgcctgc
    15601 aaagatggtt gtccctcttg tgtgggcccg ggtggagaga acggtaccgg gggaaaagct
    15661 gaaacactgg cgattttgaa acatctcgtg taaatggaaa taaaaatgct ccagtcaggg
    15721 tgaggggggc accctgactg gagcaacgat attttcccac aaatcttgca aaatgcaagg
    15781 gcgaatttct actgaaattg aattcagtac ttacttaggt tacaggtcgg agccttttaa
    15841 tttttgtgag attctccaat attgaaatcc aaagatgatc gcgaagcctg caaagatcgc
    15901 agcgttgatc ggcgtgtctg tccacatcca atgcatgcct tgtgcatcaa tgtagaatga
    15961 gaggggcaga agtgtgaaga ggatggtaaa tgccatatag gtcgaacgct gacgtaacag
    16021 cttttgggtt ttgagaagtg tatccatttc attttccttt gataggttga tttgactttc
    16081 agggagaatg gcgccgttct ctctgacgag ttttgagaat tcagggtcgt ctttcagaaa
    16141 tgcatccaca agcgctttgg tatcttggct ggcttcgcct gaaagatata caggcaggag
    16201 atcggtgatg acatcgcgtg taattttcat ggggcggatt cctctgcttt cattcgatat
    16261 tcgttcaatt tgattcgcat cctgtggacc ttgaccctgg cagaagccac cgaaattccc
    16321 agtgcccggg caatttcctc atacgggagg tcctcttgaa tgtgcatgag cagtgcggca
    16381 cggtccactt ctggcaggga ttgcaggaat tgcatgacgc cttgcaggct gagccttgtc
    16441 tcaaccgttc gatcaagacc ttcaaaggcg gcgggcaggt ttgtggtcag gtcggtgatc
    16501 cgtttggctt gtttgagctt tttgagggca aggttgcggg caatggtcaa caagtaacct
    16561 tttacggttt ccgctcggat attttcggcg gcggtgaggg cacgaatgaa ggtttcagag
    16621 gtgatgtcct ctgcttcctg ttcgtttcca caaagccagt aggcaaagcg atgcacatcc
    16681 tgggcatatt ttttgtacag ttcgtgaaag ttaacgacca tttacatata ttatccccag
    16741 agatcgaaaa tgttacaggt aaaaagaagg acagccgtct tttgacagct gtccttttta
    16801 ttgactaggc ctcgtaatcg ccggggtctt cttccacggc catcatgggt tcctcggctg
    16861 agcgtttatc aaggaattca aggctttgaa gtacaacctt ggtgtaatac cttgtttcgc
    16921 ccttttcttc gtatttttcg gttttcaggc gtccttcgag aaaaaccagg cttcctttgt
    16981 gcaggtattc ctggcaaacc tctccaagac gctcccaggc ttctatgttg acccattcgg
    17041 tggattcttt catttcaccg tttctgtctt tccaacgatt gctgatcgca acgctgaagg
    17101 tggtcacttt tttgcctgta ggggtgaact tgctttccgg gtctctgccc aggcggccaa
    17161 tcaattggac gcggtttagt gttggcatgt catccccgct tttgcttcaa ggcttttaga
    17221 tgcttgttta cgtgtcataa taggctcctt tttatctaaa agaacaatcg gttgtgtcga
    17281 acagagttat actttatgag gcaggttgat ctgtctccgc gggctttgct tcgccttcag
    17341 caacgattct cttgaggacg agcatgcctg tcttcatttc agaaacggtg tatccagcgg
    17401 gaagagcatc cagcgcgcct tccttgacct ctttggagaa gaagaacatc ttgcctttct
    17461 ggtgcaggaa gtaggtggta ccttttgagt tggtgtgtga gtatgccatt ttttctcctt
    17521 atatctaatt tggtaagtag agtgtagaac aaagtttcta gcctgtcaag cccccaaagc
    17581 acaggggaga gtaggataat agtatgagga caataattag tctagacgaa ttgaaagccg
    17641 ctcgcctctc tttcaacggg acggtgggtc tggttccaac gatgggatat ctgcacgagg
    17701 ggcatctttc ccttatccgc cgtgcaaaag cggagtgtga ccatgtggtg gtcagcatct
    17761 tcgttaaccc aacccagttt ggggcgaacg aagacctcgc caagtatccg cgtgaccttg
    17821 aacgcgattt gagcttgatc gagcctttcg gcgtggattt ggtgtggact cccacccctg
    17881 agatcatgta cccgacgggc taccagacct gggttgaggt ggaagcgatg acccgtccgc
    17941 tggaaggggc catgaggcca acccatttca aaggggtgac cacggtcgtg gcgaagttgt
    18001 tcaatgcaac tcaggcgcac aaggcttatt ttggtcaaaa ggatgcacaa caggtggcgg
    18061 tcatccgtca gatggtgtgg gatttgaatt ttcccctcga agtggtggtt tgctccaccg
    18121 tgcgcgaggc agatgggttg gcgatgtcga gccgaaacaa atatcttgaa ggggaaagcc
    18181 gtcaggcggc cacgatctta ttccgcgccc tgaccgctgc caaggaaatg tatcaaacgg
    18241 gtgagcggga tgctgaggtc ttgcgcgaga aaatgaagga agtgctcggt accgagccgc
    18301 ttgcacaggt gcagtacgtc tcctgtgccg attacaacac cctcgaagag ttggatgtgg
    18361 tgaaggggaa aaccctgctt tcaatggcgg tgctgatcgg caaaacgagg ttgatcgata
    18421 attttgtgct ggggtaaaaa tggacctccg tcagcaattg ggttttgttt tgaaaatttc
    18481 aggcagagtg ctgctgctgg ctggtatcag tcttttggtt ttaggcggca tgggtgtgta
    18541 tgatgtcctg catccgccgc ggatcgtgcc tccgggatac acactcgtaa aagagaatat
    18601 cgattttaga agcgtggacc tgctaaccga ggatggaatt cggctttcag cgtggtatac
    18661 cccgccagat gatgagcgca aaggggttgt gatcctgctg gcacatggtt acggcgacaa
    18721 ccgacctgaa tgggtgtatg ccatgatcgc caaacatgga tatggagtcc ttgcctggga
    18781 tgcccgcgcc cacggggaaa gcgaaggtac gatcagcacc attggatatc gcgaggtgtt
    18841 ggatgtgaaa gccgcgctcg attttgcctt ggcgcagccc ggtgtggatc atgtgggggc
    18901 atggggtggt tcgatgggtg gtgcaacgtt gatccgtgca acggccagcc tgccccagat
    18961 cgaagccttg gtggtggata gttccttttc ttcgctgggg gaagaggttg attttttggc
    19021 gccgtatccg ttcgtgaatc cgctggcaaa atggatgctg acttttgttc tcgatcacga
    19081 tttatacagc gccagcccgt tggagaagat cagcaagatc agtccgcgcc ccgtgtacat
    19141 cattcaaggc agtgcagata aggtggcttc gcctgattca gcgttcaagt tgtataacgc
    19201 agcgcaagat ccgcgttatt tatgggtgga ggagggtgcc cgccatttgg ggacatttca
    19261 ggaaaacccg cgcaggtacg aaagaagggt gatccgcttt ttcgatgaag ccttctttgg
    19321 agaattggcg cgtttgttcg agcaaaaaca gccatgaaaa acaggcgcta ctggcgaaac
    19381 ctggttctaa tgcttgcgtt ttcgcttttg ctggctttct ttatttttct cgtttttgtc
    19441 agtgaccagt gggcacaagc gcggctccat cctgcgcgtg tgattccaaa cgatgatctg
    19501 cttgtagaaa acggaatccc gtttcaagag atcgaattga acaccaaaga tggtgtgaca
    19561 ctttcagcat ggtacacacc gcccaaaaat ggagctgtga tactggtcgc acatggatat
    19621 aactccagcc gtgaggcggc ctggcatctt ttgtttgcta aacatgggta tggcgttttg
    19681 tctttcgatt ttcgcgcaca cgggctgagc ggtggagagg tttcaacatt ggggtacttt
    19741 gaacaggccg atgtggaagc cgcgttggaa tatgccttga gtcagcccga ggtggagcat
    19801 gttggcgcat ggggtggatc catgggcgct gtgactgtgt tgctcgctgc ggccgatcga
    19861 gtggaaattg aagcagtggt cagcgacagc gcctttccca ctttggaaga tgtgctggcg
    19921 atcaatgtcc cgtttggttt gatgaagcca tttgtaatgt tcttcggcga atatcgcagc
    19981 ggcgtggata tggacctcgt gcgtcctgtg gatgatattg cacgtatcgg tccgcgcccg
    20041 gtctttctca tcgacggttg ggagggcggc acgatcatca tgaactcccc gtatcggttg
    20101 tatgatgccg ccagagagcc aaaacaaatt tgggtggaag atggcgttcc gcacctggcg
    20161 atgcatgctt actttccaga agattatgaa aacagggtgg tgggtttctt cgatgaatat
    20221 cttttggctc attaggaatg caggtcggat tgtcaatcca acctgcattc ccaactctgt
    20281 gcctcagtag ttcaaaaaaa ttacggtccc cacttcatat ccatgccaaa ggggaggagg
    20341 gagatcatgc ctttttggcc gtcggtgtaa tacagctcaa gcctgccgcc ttgatggatt
    20401 tcaggcaggt cggcattggc tacgatgaca aaacttgcat ctggcgccca caaggcttcg
    20461 ttcatgcgct caaaggtttc agggcgtaga acggttcgat tcgtggctcc atcggcccca
    20521 gcgcggacaa gttgcaacgc tgcgcggttg accatgtctg tgccgccggg catggtggtg
    20581 tggaagaaat acaactgtcc atccggtgcg aggaagggtt catcggccag attgagcggg
    20641 ttggtatcga aactgccatt taacagagtt gtgaccgcac ctgtggctgc atccacgcgc
    20701 cagaggcccg cgctgaacat gccaaaagtg gggctggccg cataaaaagc agaaccatct
    20761 gaagtccact cggtttcgcc gcaacagatc atggctccct caccgccagt caagcggacg
    20821 agtgcgccgc cgttggggta atagaccgcg gcagatgcac cttccatgta gccgagagtg
    20881 atgagcaatt tacttccatc ggcagagtag gtttcaggcc agaataattc aaggggaaat
    20941 gaatagccgc catccagggt ttcgaatgta tttgccagca cagtgttgta ttgacccgac
    21001 acaatggaat aaaaacttaa tcctttgtat ccaaaggcga tcgtctggcc gttcggcgaa
    21061 aaaacgggat tggaaatata agtgaggtag cggttgtttt catccacctt gccgccatcc
    21121 acgagcaggc tgcgattgct tccatcggca aggacattga gtaattgatt gttggaggtg
    21181 tacaccacac tgccatccac aggtgaaacg gcataatcct ccacctttgc aggttcaaaa
    21241 gtgatctggg tgacggtttt gccgtccttt tcaaggcgat ggacttgtgt cagcccggca
    21301 ccgtcggtgt tgagaaaata gatcgaatgc ggaagcaggt ccgaggtcga agcaggaggc
    21361 gcatcgcccg attcggcggc gggagctgtc agcgcttgaa atgtcgcagc gacggcggtt
    21421 tccacaccgg caggttcaac gggcgatgtt ccacccaatg gattggcaca ggcaagagcg
    21481 acaacgaaca aagcaagaag cgcggaatag aaacggatgt ttttttgcat tttgacctta
    21541 tcgttaaaca aaacaaagac gttaaaccag taacgtcttt gttgttgaag tcagcgttac
    21601 acttgctcga agtgttgaac tggaaccacg aacagggtgg cacgtttctg gtcacctttg
    21661 gatggcagcg aatcgcgcac gacctgtatc gctgcatcca cctgcgggtc ttcgacgccc
    21721 aacaacatgg tgaccacacc gctgcgcata aaaccacctg tggaggcaac acgcgtcacg
    21781 cgaaaattcc ctgccgtgaa tgcctgtgtg agcgtgtcgg cgtcgttgtc ttttacaatg
    21841 atgatgatca atttcatgat gacttctcct taagccgaga ggttttataa accttcagga
    21901 ctttgcattt tatatttgtt cgaatctttc aacaggcaat gcaaacacag ttgcgccgcc
    21961 gacggtgacc ggcacaggcg caggcatggg catgggcgag ccttcgagcg gcagggtcat
    22021 gtattcaatg cgttgacggc aggcctcttg cagtgcggca agcgcatcgg cttctttttc
    22081 cgcgggcagg ccgatcaaca aggtggcatt gcgccgccct aaaaagcccc ccgaactcga
    22141 aaggtaggtg acggccgcac cgattgcctg cacagctttg gtcgcagaat caaggtcctg
    22201 atcctgcacg accgcaacca ataagaggtg gattggattt tccatcttcg ttctcctttg
    22261 acaatagacg cttcgccctt aaccgaaaat ggattctggt acgagcggca tttcctcact
    22321 tcgatattat cagatgtatg gagatgcttc aaccttgaaa tcagaatcac atccaaggga
    22381 aaatgaggac atacaaaatg cttgcaatag cgcaagtgac cagcatcact ggcaggccgc
    22441 gttttatcca cagggaagca gtgatggggg tgcgtgtttt ttctgagaga gttgcaacaa
    22501 tgatatttgc gcttgagcca ataatagttc cacttccgcc gaagcccgcg ccgaaagcaa
    22561 gcgcccacca aagaggggtg atgtcgatcc ctgtttctcc caaccctttg atgacgggaa
    22621 ttagggcaat ggtaattggc acattatcca ccactgccga aagagccgca acaatccaaa
    22681 tgataaggac accgaataaa acaggcgacc aacccgaggc attctcaaat aaaccaacgg
    22741 ccatttccag tgttccagca tgttccaaac cgcctaccat aacaaataga gccccgaaaa
    22801 agatcaatac actccattcc acttttttca aaatttcatg aatatcaggc tgcacccaga
    22861 gcagggcaac agccgccgca ctgagtgcta caaatgatgg ttcaatgtgg agcaaatgat
    22921 gcacaaaaaa aagcaaaatg gctccgccaa gtacaattaa aattttacgt gctgtttcag
    22981 gctggttaaa tgactcggca gggtttagct tcataacagc cttactcctg cgcggacgtt
    23041 tggataattc ctttttgaat aggtgtttca ggagaaagag cgccccagcc caggatacaa
    23101 gcacaatggg aagtgaatac accagaaagt cattgaaaga taatcctgct gcagacccaa
    23161 ttaatacatt gggcggatct cccaccagcg ttgcaacacc gcctgtgttc gataataatg
    23221 cctcagtgac aagatagggc agtgcgctga cccccagaat ttcgctgata agaatggtga
    23281 cgggggcaat caagacaacc gtggtgacgt tatccaaaaa cattgaaaga actgtagtga
    23341 tacttcccaa cagaaaaaat aactgggttg gttttccttt tgaagtacgt gccgccagaa
    23401 ccgcaagata ttcgaagaag cccgttggct caagcatggc aacaagcatc atcatgccca
    23461 acaggagacc gagtgtattg aagtcaatgg cttcaatggc agcttcttct gaataaaacc
    23521 ccatgacctt tccgatccca atcatcagaa cagaacctgc aatcgcaatt atggaacggt
    23581 tgattttttc ccagaaaata agaatcaagc tgcctaaaaa aataactgta gagaggatgg
    23641 cagggaatac catcattttt ccaccctggc ccatgtggaa agaatggtcg atccaatatc
    23701 cacccgtgat gcaatgccaa tcaattgacc atctttggaa acaacgggaa tatcatgcag
    23761 gttttgttgc aacatcagtg cataggctcg taaaagacca caatcttctt caactgtaag
    23821 tactggtctc attaacgact tgacagattt gttcagcgtt tttgcagatg ggcgggtatc
    23881 ttccactgca ccaaaatcat ggacaaaatc aacgtctaca acgaaattga tgaagtctgg
    23941 catttcgagt ttgagcagat cacgtatacc aatcaccccc acaggcttat tgtttttatc
    24001 tacaattggc agcaagccaa tatgcttttt tgtaaaaata gctgcggctt cacggatggt
    24061 tgtcgtgacg gggattgaaa caacctgacg cttcatgcat aaagttacat tcatggaatc
    24121 tccagaaaag gatggttgac ctgtgatatt ttgtatgatg tcatttgggg gtaacttttt
    24181 tattttaact tatctttttc atcagacctg acgtgcgaat ggcaataatg accagcccga
    24241 caaatgtggc aggtgtaaaa aaagctgcaa agaggctgaa aagaggcaag aatgaacttt
    24301 gtgtcgcctg taaaacgata taaccaaggt agatgctgta gtaccccaat aacaaggcac
    24361 cttccaattg cgaaatgcga ttatcaatgt aaaaaatcgg cagagtgaca agtgccgcaa
    24421 ataccatcac tggaaaatca aattgcagca cctgttcagc cacagtaatg ccgttgggtg
    24481 agaaaaacgc cccggcaccc aacaccccga gcaggttaaa gatattactt cccactgcat
    24541 tgcctacggc aatatcgctt tcattcttta atgcagcaat gactgatgtc attacttcag
    24601 gcagagaggt gccaactgca acgatggtca atccgataac caattcactg acaccgaggg
    24661 attttgcgat tgcaacagct gaatccacca gccagtttga acccagaata agtaacccaa
    24721 gcccaatggc gatagaaaca aagttaataa caatcccttt ggcggtgcga ggctctttct
    24781 ttgcgaactc tttgttgtac tcattttgaa cttcactgct ttcaatcctg ctctgtttca
    24841 aggcaaaggc tgtgtatgcg gccaggagta ataaaagcag accaccatca atcgcgccaa
    24901 tatttccatc caatgaaagt gcaagtacga ataaggagac gaccagcatg atgggggcat
    24961 ccttgcggat caattgccct gcgattgcga tcggcgctac aatggatgtg accccaagaa
    25021 taaagagaat gttgaatatg ttggagccaa gtacgttccc gagagtcaag tcaacctgtc
    25081 cattggccgc ggcttgtagc gaaacagcaa tttcaggtga tgcggttcca attgccacaa
    25141 tggtcaggcc aataaccagg ggagaaatgc caaaggccgc agccagccgt gatgcgccgc
    25201 gcaccagcag gtctgcgcca agaatcaaaa gaatgagccc agcgaggaaa aggagaaaag
    25261 agtttgtcat gtcaatgtgc ttccaatgga attgtccgaa atttttatac catgactttt
    25321 tccgcctgag attaattccg ctttaaatac agggctggtc aatacaaatt gaatcttgtg
    25381 attctgagtg cagaaaaaaa actatttttg cctttccaaa tattcctgca cctcgatcat
    25441 tggcgggcgt tccagttctg cgatctgctc cacgtcgaga gaataacctt tgttgttgta
    25501 gcggatcagt ttcatggatc gattcacatc ttcgagcatc acacggccaa cccgattttc
    25561 caatttgcgc atcacggtct gaatgatcgc gaaggacatt ttgctcaatg cctccagcga
    25621 ttgattatgg tggatgcgtt cgagcaaatc cacctgtgca atggcgttca ggccgtagcg
    25681 ttcgtaaata tcaatgagca gacctgtttc aacaccatac cctgagtaga acggtgcgcg
    25741 ctccaatgct tcgcgcctgc ctgcatattc acctgaaagc ggctgcacca cacccgaaag
    25801 ctcggggtaa aacaaattca acagcgggcg cgccgtcagt tcggtcacac gcccaccgcc
    25861 gcctgcctgc attttttgtc ccacgcgcag cggcctgcgg taaaagcctt tcaccaattg
    25921 cacctgtggg ttggttaaaa gcggcccgat gattccatac acaaagcgcg gatgaatatt
    25981 gacgatgtcg gtatcgatcc acacaatgat gtcgcctttt gtcaccaaca gacttttcca
    26041 caaggcttcg cctttgccgc gtcgtgcccc caaccgtccc agcaattgct gatggatgta
    26101 aacaggcaca ccttcttttt ttgcgatctc gcgggtgcgg tctgttgaat ctgaatcgat
    26161 caatacgatc tcatccagca gtggaatccg ctgcaataat tccttcttca tcattttgat
    26221 caccctgccc acggtttctt cttcgttaag tgcaggcagg gccaggctga tgctcagccc
    26281 ctgtttcttc tttagttcga tcaattcgcg caattgattg aattcatctg catgaaatgt
    26341 gttttctgca aaccacttgt ccaccagaat ggagatcgcc tccacgcctg cgctttcatc
    26401 gtacacggca tgcggtatcg gccttccgct cttgaccgcg atcacggcac agggcgcttc
    26461 acgcaaaatg cgatctgcca caggtcccag tgaaacggaa ctcgttaaag gctggatggt
    26521 cgttcctaaa ataatcacat ccttctgcct ggcatggtcg aggatgaatt gcgccgaatc
    26581 atccgtaacc tcgaactgct tctgcacctc aggcatttgt ttcagaacgc gttccaaccc
    26641 cttgaatggg gcatccgtcc ctgcttcggg atcatttgtg cggcggagat gcaatgccgt
    26701 gacgccttga acatgcagac ccagccccac acgcagcgcc aactctgcat gcggcccgcc
    26761 tcttactgga acgagaacct gcttcggttt actggaaatt tttccgcgta ccagggcaat
    26821 gtcacatggc gggcggctta gtgcatcgct aggggtgacg cacatggcct tgaaatgaga
    26881 ctcatactca aggcaaagca gatcgggttt ttcatcctgc agcagttttg caagttcatt
    26941 ccacggttcg tgcgatacga tcacctgcga tttgctggtg gtgcgtttat ccctgccgaa
    27001 gacgcgcagc agcctgcgca aggcccgcgc cgccgatgcg cccacgctca acgattggtc
    27061 ctgcggaaca accactacac ccaccagcgt gatctctgca tcaaattggc gtgcggcatc
    27121 caaagcgtga aggcctggcc cttcatggat gaatggaaca ataatgttat gaataggcgt
    27181 gaattcagag tgcttgaaca tgaggtttcc tttttgttat ttcaataatg gcgcaatgtg
    27241 ctgacgatag atcgcctccc agcgaaaggc tgcccgcgcg cgcatggaca aacgcgcagc
    27301 aggggaggat tggaaatatt ccgcgatcat ggctgagatt tttacaggat cctcatcagg
    27361 ggagaaaaat gctgcgtcat ttaatcccag ctcgcggagt ggaggaatat ccgcgcaaaa
    27421 ggcgggtaaa tggctgaatg cggcttcgat caacgggatg ccgaatcctt cctcgcggct
    27481 tggaaataaa agcgcatccg caacacgata aaaatctgcg atgacctcat cgggcaaaaa
    27541 agagtcggtc aactcgctga ggaagtgtgc ggaatgctgc aagccaagct gggttcgcaa
    27601 actggaaagc gactcaaaat atttttggtt gttggcattg tgcggaccca gcggccccgt
    27661 gaccacgagt gcagcatcgg cgaatcgttt gcgtaattct gccagcgtgt gcagagccag
    27721 ttcaatattt ttgcgcggcg tcacgcggac aggcagcaaa aggatcggcg tggcatccag
    27781 taattttgtt ttttcaagca ggctttgtgt ttgtgattcg agtttgtaaa aacgcgccgc
    27841 atccacaccg tttgggatga tctgaatttc atcgggtggg atcttcatca acgttgataa
    27901 ttccctgcgg cgcaagtgtg aaacgaccac atgcgtggtt ccaccccaat cggtgcgcag
    27961 cagatcccag ggatagccat catgtagttc gggttgatag cgcggggttg tccacgcgag
    28021 atcatgatgc cagaggatca agcgcggcag ttttttggaa acatgcagtt ggtacagcgc
    28081 ggcggtgagc gccagatttt tattcaacga acatacgttg tgtgcgatca aaatatccac
    28141 atcggccaaa gcggtttgca gttgaaatac cagttcgtcg cgcaaagatt caaagtcttg
    28201 tgtcacttcg ccatgatcga gttgattttt catgtgagtc acacgttcgt gccgcgaatc
    28261 ggcaagcgga atttcggtga gaagaatctg gtcgctcagc gattcgccgc gtgccgcgat
    28321 caagcggaca gaatgtccat ctgcagacat cagccgcgca tgatgaccga tcacactttc
    28381 cacgccgccg acaatgggcg gaacggaata atgcagaatg gcgatgttca tacctgctgc
    28441 cccaaacaat ctgcaaggat cgattgcaat cgccgctcca agacggtgaa tgaaaagtac
    28501 cgctttgcaa gctgatagtt ttcctccgcc catgcctgcg cttcggcggg attcaacaga
    28561 atttctctca cctttttcaa ggtttcgaca ttgatgaatc cgtcgaacca cacggcgcga
    28621 aatcccttcg gcttgatatc cacctcgtaa atggaataat tgttgaccag aatcgggcgg
    28681 cgatagtata cggcttccaa aaaagcattg ccaaagcctt caatgctgga agggtacgtg
    28741 atcaggtctg catgcgggta cacatcgcca agtgtgtaga tctttctgcc gtccttcgtc
    28801 gtgccgcgtt catcctgcac ctggtccgct tcaaaacgga cggtcacatc aagtaaatgg
    28861 gcatattcgc gcacacgatg ttcataatct gaaccttcat cacccgaggc gtgtgagatg
    28921 accagctttg caggcacacc gagtcggcgc accagttcga ccgcatgttc aatgcccttg
    28981 cgttgaatga cgcgtgtggg ttgcaggaaa aaatactcgc cctgctcaat gccaaaatct
    29041 gcacggatgt tttgggtgta ggcatcgggc gcgggcggcg gagagtcgaa atccattacg
    29101 ttggggatca cgcgtgaggt cacgccataa cgcgtggcga tctgttgtgc ttgcagtgaa
    29161 ttgatcacca catgtcgaat cgacggcagg gtgggcggga aggccgctgc aagataatcg
    29221 ccgacgcagt tgacttgaaa ccgctggcgc tcccaatgaa agtcatgatg atgtgcaatg
    29281 gtggggtatc ccgtctcggc gatgaactct gtgatggcga gaccaagcgg cagattaagc
    29341 gggatcgtca ctgcattttc gaccaccaaa atttccagat cgaatcggcg cgcaaattta
    29401 tacaactctg ccttgatgtg ttccttcaac tcgtggacgc ggcgcgtcat ctgcggcggg
    29461 cgcacataga tcgagaaaaa atctttttgc aaagccgcga tttcagggtg ttcggaacgc
    29521 gctttttggg tcaccgtcca actgcccgaa taagcctgct gatttatttt ttcaatttca
    29581 ggatgacgat aaaacgcctc tggcgtgacc tgagactggt cgctgctccg atcgcattcg
    29641 ccagcaaagt agaaacagcg gtgcccaagc cgttcgagca cggttgccca cttttgcgtt
    29701 tcaagcgaaa cgccgtcggt ccctgcgaaa cgggtggaaa tgaaaccgat gtgatgagat
    29761 gggtttgtca taaaacacct ttaatttttt cagtgcagtc atacttgatt tattttcagc
    29821 cattgcactt gatagggcag catcctgaca tctgaagcct gaattgcctg acccgtaaaa
    29881 aggtcaattg ccgatttatg ccctgttgag acggaaatga tttgatcgca gacgttgtgc
    29941 aggcacaaca cgcgtgatgc accatcggga gaaaggcgtt caattgcaaa aatggacggg
    30001 tgcgaatcca ggacggtctg tgccccatgc ggatggaaag ccgatgaact gcttctggct
    30061 ttcaacagct gagaatactt tgtgaaaacc tttgagcgca atgaatgttc gtctgtgagt
    30121 tcgttttgta attcatccag ttgaagtttc tcgcggttga tcgtgcgatt gcgccccgtt
    30181 tgtcttacac cttcaaacca tccgcgagac ccgaacaggc tgtgaaaata gattccaggg
    30241 atgccgatca gcgcaagcat aatggcttgt gaggcgataa aacggttgac ctgcagatcg
    30301 atcggctcat gtgcatttgg gtcggaaagt gcatcgaaat aattgatatt catctcatac
    30361 gggctttgtg tgccgtccgt gttgtgtttg taggagatca agccgccatg ttcaagggtc
    30421 ttgtgaacca atgcgttaat ctcttcgttc gagaggatgc tgcgcacagg attgattccg
    30481 ataccgtcat gcgaggcgag gaaattgaaa aaggtcgttt tgtcagtagg cagggccaaa
    30541 gtccttgccc agtttgacag tttgcctgca ttgcctgtgt gaaaggtgtg caaggtcagc
    30601 ggcggcaggg caaagttgta aacgagttgc gcttcattcg tcccatcccc aaaataggag
    30661 atgttatccg cgtgcggaac gttggtttcg gtgatgagtt gaatatgcgg agcgacttcg
    30721 ttcaatgcgg cgcgcagaaa ttggatcaca tgatgggttt gcggaaggtg aatacaggtt
    30781 gtgctgattt ccttccataa atatgcgatg gcatcaaggc ggataaaact tgcaccgcgt
    30841 tcagcgtata tgagcaaaat atcaaggatt tccagcagta cttcggggtt tttgaaattg
    30901 agatcgatct gatcttcgct gaaggtcgtc caaacctttt tttcacccgc tgctgttttg
    30961 aacgtcgtca acaatggcaa tgcccgcgga cggacaacct ttgaaagatc agggtcacct
    31021 tcgaccgtga tgaaataatc acgataatgt gggtcgtcac gcaggaatcc ctgaaaccat
    31081 gcactctgag atgaaatatg attgatcacc ccatcgaaca tcagcctgaa actttgcatg
    31141 gacgatacat catcccaatt gccaagtgac gggtcagttt ggcgataatc caccactgaa
    31201 aagccgtcgt ctgatgtcca cgggtagaag ggcaggatgt ggattccgct gacaatatcg
    31261 gttaagtggg ttttgcaaaa tttcctgagc gtttgcaacg gcctttcggt cggtgtttga
    31321 atctgatcgc cataagtgat gaggatggaa tcgcgttcag ttgatttacc gtcgcggggt
    31381 tcgatgcggg tacgataatt gttgagcaaa ctatgtgtga gttcgagcag gcgcgttgag
    31441 tcattttccc cgtatagaaa agtgagatgg tcaagcagtg ttatggtcat cagtcctttg
    31501 tggctggtga ataaaccttg agtcctcgta aatttttata ccatgatttt tgaggcttaa
    31561 gattaaatct cctgtaaaac gaaagtggtt catggagtct caaggcctct gtttaatgaa
    31621 tacggtatac tgcccgtgag gaggaatcat gccagacgat aaaaatatga tttccatcat
    31681 tcttgcagac gatcatcaaa tggcacgcca ggggattcgc tcgattttgg agaaagctcc
    31741 tgattttgag attgtagggg aagcaaaaga tggtgatgag gttcggcggt tggtcgctgc
    31801 tttgcgccca caaatccttt tgctggattt gaaaatgccg aatctttccc cggcggaact
    31861 tgaaaagtgg gtgcggatca actaccccga aaccaacacc ctcgtattga ccgggcatga
    31921 ccgcgatgta tatctcgcaa acatgatgga tgcgggagtt tcagggtatc tggataaaaa
    31981 attgcgcgca ggccaattaa tttcagccat tcgccgcgcc gctcggggtg aaatcttttt
    32041 cgatgaagag cagattgaaa gggcgcgccg ttggcgcgag gaggtgggta acaagtgggc
    32101 gagcctttca gaacgcgaga aggaaatttt gcaaaaactt accgaaggcg cagagaatct
    32161 tgccattgct gaaatgctct gtattacaac aaatacggtt gagaagcatt tgacgaatat
    32221 ttacaaaaaa ctgggtgtta aatcacggct cgaagccgcc atttggtggc tggaaaagag
    32281 tggggatttc cgtaattgat gaggacattc aggaatgcta taacctcctt tgctaatgtt
    32341 gaatgattgt caagctattg ttatattcgg aatattcgct tgccaagaat aatattcaat
    32401 gacaacattg tgtacactcc acgcatacgc agcaacaccg acacgttcct gtgggagtgc
    32461 cttggtttct gggcctattg ctgtcccgat tggtgtatcc aacacatttt gtacaggcgc
    32521 gaattagata aatcgagaaa tagttctgga ggaaaatgaa accattattc aaaataacat
    32581 ctggtttttc tcttgatttg gtaatgatat tgattgttgc aggcatactt gtctcctgtt
    32641 ctcaaaaatc agtagttgtg ggctcaaccc ggaatcaaca attgcccatt gttgttcgat
    32701 caactgaggt cacatcaatt gagcaacata aaggatttga aactacatat ggcatcatag
    32761 ttgcagtaaa agcactgtgc aaagatgatg tgaacactat actctatttg aaaactaatt
    32821 ttgatgcgag cctctggcat ttaaatgaaa aggaatttta cccatttgga ggagcttatt
    32881 ttgagacaag tattttgttt ctagaaaatg gcgaaatgtt ttcaatgtat gcaagtggaa
    32941 aacgtgatga gccaattttt gatttgcaaa ataacatggt cagtacaatg caaaccttcg
    33001 tttttccgaa aataccttct gaagagtcca catttacaat caaggcaaaa gtatctttac
    33061 atgatttgcc tgttacttat attcccccaa taaaagttga ctttttggag cctggaatta
    33121 tcgaaattcc aatggagtat attgtgtctg cagaaattgg agagtgtcca taaagttaca
    33181 gtgacacgat tgtttttagg caagcctggt acggagtggg tgacaggttc actgattaag
    33241 gagaataata aggaatgtct cgtgaataaa aaaaacatgc tcgtttctgc gtttgcagca
    33301 acctttgtag ttgttattgc aggaatagtt gcgatggtgg ttttatattt ccagcccaag
    33361 acagtcatcg ctaaatctgt agagccggtt tcaaactacg ctgaaagttc aacggttgtc
    33421 aacgttccat cgctagaaaa ttcaaatgcc gccgaagtga aatatccgct tgcccaggaa
    33481 ataaacggta tcaaaatgga agtgaccggc acggctatgg aaggggagta tttcaccaca
    33541 gatgtatgct acgatcttcc agccccaaaa acagggtatg aatttacact gggaggccag
    33601 acccaaaaat ccattgcatt gaccaatgct aaaaatgaaa ccatcatgat atatagttgg
    33661 aagatcattg gaggtttcaa tcaagatgca aatggaaact tccaggggaa ttgcgcccgt
    33721 ttatatttcc ctgttttgtc tgataccaat ctggatggtc tgacactcac catttcgcgc
    33781 atgtccactc ctgtggcaga ttttcccgat tgtgagaaag cccagcaaaa gttggatgag
    33841 gcaaagcaaa aaataaaggt ggaatgttat gagaatggcg ggtttggcgt atctgaaaag
    33901 cccgatgata tgagcgatca agaagcgaga gaaattgcat tagaggcttt tattgaaatc
    33961 gtggaaggtc cctgggtatt taagctgaag taatttgaac aaaaagagcg gactgttttt
    34021 agcagtccgc tctttttaca tctccactat acacgctgtt tcttctttta cgatcacgct
    34081 ttccccattg gggaaggtta ccaccgtttg ct
//
LOCUS       DAOQLC010000037        33783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_404_length_33783_cov_29.938360, whole genome shotgun sequence.
ACCESSION   DAOQLC010000037 DAOQLC010000000
VERSION     DAOQLC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_404_length_33783_cov_29.938360"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PL000_08565"
     CDS             complement(<1..698)
                     /locus_tag="PL000_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNC88992.1"
                     /translation="MAYTNVKVPEGGAKISIQDGKLNVPDNPIIPFVEGDGTGRDIWR
                     ASVRVFDAAVEKAYGGKRKINWMEVYAGEKSFKMFQSWLPDETTEAFKEFLVGIKGPL
                     TTPIGGGIRSLNVALRKLLDLYVCLRPVRWYKGVPSPVNHPEFVDMVIFRENTEDIYA
                     GIEFQHGTEDNKRFKEMFKEAFPKEYAKMRFPETSGIGFKPVSEEGTERLARAAIQWA
                     LENKRKSVNFVHKG"
     gene            complement(956..2239)
                     /locus_tag="PL000_08570"
     CDS             complement(956..2239)
                     /locus_tag="PL000_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88993.1"
                     /translation="MSKENSRYRWFVVVVFFFFMLLHQTDKLMIGSMQVDISKDFNLN
                     NQQWGLINSGALIVATILYPIWGYLYDRYSRTKLLSLASFIWGATTWLNAIVKTFGGF
                     LTTRASTGIDDSSYPGLYTLIADYFGPNLRGKIYGILQLAQPIGYLVGMILALMVAPA
                     IGGWRSVFYITGSLGLVIAVLIYFGVREMPRGQAEPEFENMPEMQTFKFSWTEAREIF
                     KKKTMWFVFLQGFAGVFPWNVITFFFFAYLEKERGYDANGILFTMAPVILILASGYFI
                     GGALGDMAFKRSNKGRIVVSSIGVLLGALFMYLAITTPVDAKNQFFILMCLTAVFMPL
                     SSANVIATVYDVTVPEVRSTAQAVEYFIENAGAAFAPILTGYIADLYDLKTAILIICT
                     AAWLLCFVFYLGAIFTIDKDSHDLREQMAARAKSM"
     gene            complement(2306..3535)
                     /locus_tag="PL000_08575"
     CDS             complement(2306..3535)
                     /locus_tag="PL000_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HNC88994.1"
                     /translation="MRVLHFADAHIDMANYGRHDPQTGLPMRVLDFLKSLDTIVDTAI
                     SQKVDLVIFAGDAYKDRSPAPTFQREWGRRIMRLSQAQIPTLLLIGNHDISPAVGRAH
                     ALQEFKTLQVPFVKVLDQPSFLKPEDLWELPMQVIAMPWVTRSGLMANLEMSGTETEQ
                     IYSNIEERIASLVDGWIEEANENLPIIFTAHASIEGAKFGGERLVMLGNDLVLSGSLV
                     KNQRFNYVAMGHIHKPQDVNEGHQPPVIYPGSIERVDFGEAKEDRFFVIAEIESGKET
                     QVEWVQLTGARKFIDRRTVLRSNENVTEALKDALPSLKEMSEAIVRLSVEYPREWDTL
                     IDETALRKYTEDAFEFHLVKRPQSEARVRIPEGQVVSSLSPLDLLAQYFDAAKVKDAD
                     ELNKLAQEIISEDTLEN"
     gene            complement(3627..4985)
                     /locus_tag="PL000_08580"
     CDS             complement(3627..4985)
                     /locus_tag="PL000_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNC88995.1"
                     /translation="MKSKFNKYWLIPIVLLLAVGIYYLPPVHSRLAWRVDALRVQVIY
                     FFNPPDEVVFEPSGETSPLTIETAIATARAEYMLTLTPQYTPTVTPTTGPTARPTATS
                     TPLPEAVNLEGVVYVDQHERWNYCGPANLTMALNFWGWKGNRDDVAKIVKPGSGDTKV
                     NFIDRSKDDKNVMPYELVDFVNENTEYRALMRHGGDENLVKRLLAAGFPVVVEKGYYE
                     HDTTGKISWLGHYLFTTGYDDARGGFIVQDAYTKPGKNLLSDYATYLEGWRAFNYQFI
                     VVYPAEREAEVLTLLGPWVDENWAAQHALELAEKDIQSINDNNLFFAWFNKGTSHVLL
                     KQYVDAAAAYDQAFSTYANWDEKEQERPYRIMWYQTGPYFAYFYSARYADVISLADTT
                     LFDPANKKGRTLEESLLWRGRAYYMSGNTQAAIDDYRAALRIHVNWPPAVQALQDLGL
                     QP"
     gene            5072..5299
                     /locus_tag="PL000_08585"
     CDS             5072..5299
                     /locus_tag="PL000_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC88996.1"
                     /translation="MTTQTKQHIPWYLWPFAALWKLLAVIVELTGRFVAMVLGLVLIL
                     VGGIVSLTVIGAIIGIPLAIIGLLLLLRGIF"
     gene            complement(5344..6021)
                     /locus_tag="PL000_08590"
     CDS             complement(5344..6021)
                     /locus_tag="PL000_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNC88997.1"
                     /translation="MKKFPYTPLQIAVHLYAWSGLALLLLDYFTGNLSPNPIQDLEQR
                     TGRHAITLLVLSLLCTPINTIFKWSEPIKRRRALGVYAFLYATVHMIIFVDLDFGLAW
                     SSVIKEVIEKPRLIVGVIAFILLIPLALTSFDIWKKRLGKNWKRLHQLVYLIAPLVFL
                     HYVWSKKGDLLLLQGEILKPLIYGLIIGIFLVLRIPPIRKAVASFSTRSLALLNKKNP
                     QPEVDTP"
     gene            complement(6087..7040)
                     /gene="msrP"
                     /locus_tag="PL000_08595"
     CDS             complement(6087..7040)
                     /gene="msrP"
                     /locus_tag="PL000_08595"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNC88998.1"
                     /translation="MTDLFKTVPIRSSEITPKSVYLSRRDFMKAATLTTGAALLTACA
                     PSVAGAAEDNAPVPTSNATDELGDPVNSYTDITNYNNYYEFTVDKQQVADEARGFNLA
                     PWTVEVGGLVNNPKTFGIEDLLKLFPQEERIYRLRCVEAWSMVIPWTGFTLASLLEMV
                     EPTSDAKYVRFETVYRPEEMPGQKSPFYPWPYQEGLRLDEAMNDLTLLATGLYGEPNV
                     AQNGAPIRLVVPWKYGFKSIKSIVKIELVAEEPSTLWSNIASNEYGFYANVNPDVDHP
                     RWSQASERRIGELNRRPTLPFNGYGEQVAHLYDGMDLSVNY"
     gene            complement(7182..8405)
                     /locus_tag="PL000_08600"
     CDS             complement(7182..8405)
                     /locus_tag="PL000_08600"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC88999.1"
                     /translation="MKSTFRALRDKKFAALLLGQTFSRIGDFLFQIALAWWVLEKTGS
                     ATAMGTVLFFSTLPMVAFVLVGGVVVDRMRRGLLLFISDTARCFIMVIGAWLAFTDQL
                     VLWEIYVIAILFGFADAFFLPAYNALIQQIVAEEDLPSANSINSLSMQFGRVAGPALG
                     GLIAGFGGTAVAFGFNALSFFIGALTVIPLLNLPVPASSEESGFTLQHFKDDLRDGFN
                     TIFSTPVLWVSIIVFAFANITLAGPYSIAMPFLVQEKLGGDEKMLGFIYSIFPIGYAI
                     ASLVMGGFRKIRYRGILLYVCGAIAGIGLGVFGFGLPLYLLVIAALINGAALEIDGLI
                     WTNLMQEKVPPEKMGRVSSVDSLGSFVLLPIGYAFTGWLIDTIGVSSVFIAAGVVTAL
                     VSLLPLLHPTIRKLD"
     gene            complement(8525..8896)
                     /locus_tag="PL000_08605"
     CDS             complement(8525..8896)
                     /locus_tag="PL000_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC89000.1"
                     /translation="METTLTVTQEKIQAHIPITVFHLQGWLDARGEETLLAAARDAYS
                     EGARHLVLDLAGVETLTSAGMRAMHKVYKLFTPTEERMKRLHVAISNAPSQVNNILGI
                     TGFLLNIPNYRNIQSAVESFK"
     gene            complement(8956..9813)
                     /locus_tag="PL000_08610"
     CDS             complement(8956..9813)
                     /locus_tag="PL000_08610"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNC89001.1"
                     /translation="MANVKITSSLVSVQWLAENLEAENLIILDASMKPVTPVGNASSD
                     QAGCIKGALRFDFDNDIREKNTDLPHMMPTAEFFTEEMQKLGINQNSAIVVYDYVGVY
                     SSPRAWWMFRAMGHEQVAVLNGGLPAWKQAGLPCGEQAETKASRRGDFVAHPQAGLFC
                     DSTHVREALADSAFAVLDARSPGRFKGVEPEPRAGLRGGRMPNSLNLPFADVVVNGSM
                     QPPAQLESIFSKLVKKEQKLIFSCGSGVTACIDALAAELAGYQNISVYDGSWAEWGSP
                     SSGLPVEQG"
     assembly_gap    9929..10028
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10089..11276)
                     /locus_tag="PL000_08615"
     CDS             complement(10089..11276)
                     /locus_tag="PL000_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC89002.1"
                     /translation="MQAASEISNVNRLSKRVAELKPSGIRKFFDIVATMKDVISLGIG
                     EPDFTTPKPILDAGIRSLQNGETHYTSNHGKVELRQGVAENLQKLYNVKYDPLSEVVA
                     TVGVSEALYLTFTAILEPGDEVIIPTPCFVSYQAEVILAGGVPIEIPARLENNFTIDP
                     DDIRKAITPRTKVIFIGYPSNPTGAVAPREVMVEIGRIAEQYNLIVVSDEIYDRLVYD
                     FEHVCFPALDESLRRRTVLLGGFSKAYAMTGWRIGYACGPADIIQGMVRVHQYTIMSA
                     PTTAQDAAIEALKNGEPHVQEMVTEYNRRRRLLVDGLNRLGLSTFEPRGAFYTFPNIQ
                     ATGMDDETFAEALLREEGVAVVPGNAFGPGGEGFVRACYATEYSQIEEALRRMERFMS
                     RHG"
     gene            complement(11286..11495)
                     /locus_tag="PL000_08620"
     CDS             complement(11286..11495)
                     /locus_tag="PL000_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89003.1"
                     /translation="MEAVECRSDTEYAERPLSLLWRGARYEIAEIISRWRGPGEKGFC
                     VKTTDGFTFELTYQEISDDWHVQPI"
     gene            complement(11498..12541)
                     /locus_tag="PL000_08625"
     CDS             complement(11498..12541)
                     /locus_tag="PL000_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC89004.1"
                     /translation="MAETLKIVIPMAGWGTRMRPHTWSKPKPLVSVAGKTSLEHLMDM
                     FKTLPEPENVEYIFIVGPYLGELQVPAFIKEKYPNLKAHYVVQHEMKGQSHALALSRE
                     YLRGPMMVCFSDTLMETDFSFLANEAADGVAWVMPVPDPRRFGVAELDNEGWVTRFIE
                     KPQSMDNNLVVVGCYYFKSAEKLLAAIDEQMQRGIMLKGEYFLTDTITVMIEHGAKVR
                     TNQISTWLDTGTIEATLDTNKTLLESWSAGAFERSNVEALKRSNVNIVEPSFIDPSAE
                     ISNSTIGPYASIGANCKIENCQIAESIIEAGCEIKDAALSRSLIGRQARVKARGDGHV
                     MQLNIGDTSSVEL"
     gene            complement(12789..14507)
                     /locus_tag="PL000_08630"
     CDS             complement(12789..14507)
                     /locus_tag="PL000_08630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNC89005.1"
                     /translation="MLKNLPSTPEAILAMEWPDYEPHFKALQSVELTAANIGEWLSDW
                     SALADRLDEQYTRRFVATTQYTTDEEIEKNFNHFIESIQPNARTEEQKLKEKLLASGL
                     QPENFKMGLKKMRAEADIFRQANLELLTEEQKLNGEYDKILGAQTMMWEGEERTIAQM
                     FPLYQDKDRAVREKAWKLVAEVRVQNREAIHSLWKKYMDLRLKITKNAGMSNYRDYMW
                     AQRFRFDYSPADCKTFHDAIEEVVVPAATRLYEKRRKKLGLETLRPWDQMVDTSNRGQ
                     LKPYQTIEEFKSRSKDVFTKVDPKFGEYFQVMMDNDLLDLESRKNKAPGAYNLSYGVS
                     QLPFIFMSNTGTPMDVDTILHEGGHAFHAFEAAVVPYFHERAENYVPAEFAEVASMAM
                     ELLASPYITREHGGFYTKGEAARARFEHLEGIVTFWPYMALVDAFQHWIYENPQISSD
                     PVQCDKKWGELWDRFLPGVDYSGLEDAKTTYWHRQGHIHTTPFYYIEYGLAQLGAVQV
                     WANALKDQKKAVENYRKALALGSTVSLPELFETAGAKFSLDAKTFKVYVDLIEEQMEK
                     LEQMEE"
     gene            complement(14521..15084)
                     /locus_tag="PL000_08635"
     CDS             complement(14521..15084)
                     /locus_tag="PL000_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNC89006.1"
                     /translation="MELKLLWEIFVLFSRVALFSWGGGPASLALMQREVVTAGWATTE
                     EFADAVAVGNALPGPIAPQVSAYVGYKMSGLWGAVSATMGTVFPTTLLMLMMVIYFFS
                     IKDSPAVAAMLKAVRPIVVGLLLWTAYTMAYSVFSVSKVGWAKGLVQNWDKVGIAIAA
                     FTLLTLTEINPVWLVIAGAVLGFIVYK"
     gene            complement(15086..15547)
                     /locus_tag="PL000_08640"
     CDS             complement(15086..15547)
                     /locus_tag="PL000_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC89007.1"
                     /translation="MPNMYIRFLFLGYRIFCFLFRPVRMGVRVMMIQDSKVTLVRQTY
                     LPGWFMPGGGLKRGETLDQAARREAYEETGAELGEIRLMGAYTSFKDWKTDHNLVFVC
                     EDFKITGQPDSEIAEIQSFPLNALPEGLWPGHVRRLEEYKAGKPNPQFGEW"
     gene            complement(15540..16523)
                     /locus_tag="PL000_08645"
     CDS             complement(15540..16523)
                     /locus_tag="PL000_08645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC89008.1"
                     /translation="MQYTNLGKTGLKVSRLCLGMMTYGSKQWREWMLDEEQAKPFVKR
                     ALDAGINFFDTADVYSLGESEKITGNVLRHFGVKRENVIVATKVFSTMSEDVNDRGLS
                     RKHIMDSIDKSLKRLQMEYVDLYQIHRWDYNTPIEETMEALNDVVRAGKARYIGASSM
                     FAWQFMKALHVSEMNGWSKFVSMQNHYNLVYREEEREMIPLCKDQGIGLIPWSPMARG
                     FFAGNRKRGGGGETTRAQSDPFANELYFREEDFVIAERAAGVAKSHNASASQVALAWV
                     LNKPHITAPIIGSSKIEHLDQAIAALEIKLTADEIQLLEELYQPHPILGHA"
     gene            complement(16555..17130)
                     /locus_tag="PL000_08650"
     CDS             complement(16555..17130)
                     /locus_tag="PL000_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89009.1"
                     /translation="MTFSSSHDPAHLYFITASVIEWKHLFAMPEYAKIPMDSLAWMQE
                     QKRILLFAFVIMPSHIHAIIKPESDSIGNILQQFGSFTAHEIIKMLKAENKPELLNVF
                     HQKRRDERHEHSIWQDIQAKNVYSTEFLWQKIEYIHQNPIAKGWTLVEDRADYAFSSA
                     CHYDNGRKPLIPVTDINEWLSPPPPRTAVGA"
     assembly_gap    17160..17259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17393..19588)
                     /locus_tag="PL000_08655"
     CDS             complement(17393..19588)
                     /locus_tag="PL000_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC89010.1"
                     /translation="MEFLKKLNAQQKSAVTAADGPVLVVAGPGSGKTRVLTQRIAYLI
                     ASEGIRPWQILAVTFTNKAAKEMDQRVKAMLNEQATEGMMLGTFHSICARILRREADH
                     LPLESNFVIYDSDDQERIVKAIIRELNLNEKLYRPASVHASISRAKNELIGAEDYPIN
                     TYRDEVVKRVYVEYQKRLISMNAVDFDDLLVYTARLLEDVPAVRDRYAQRFRHVLVDE
                     FQDTNLAQYALVKHLASHHKNIFCVGDPDQSVYAWRGADYRNIKRFEQDFPDAQVVLL
                     EQNYRSHQNILDAATSVIDRARNRHKKRLFSDRGPGEKIFFYEARDDYGEASFVVDTI
                     AQLVASRQFEPGDCAVMYRTNAMSRLLEEAFLQARLPYKLVGAQRFYGRREVKDVIAF
                     IRLIHNLADEASLDRIINVPPRGIGEKTLTTLHLTARQNNTSPGAILLDLARGSDSPF
                     YKSFTGRAALPLADFGGMLATWRAMAATATPAELFEKIVKDINYKEYIVEDDSDESKD
                     RWENVQELKRLALEYSTRTLDEFLENIALVADQDTITDMNAPTLLTLHAAKGLEFGAV
                     FIVGLDDGIIPHSRSFDEPEQMEEERRLFYVGITRAKDKLYLLRAIQRGGRGMSEETY
                     PSRFLEDLPAELLVGKTRTGKPTTSRGLYGRPDTSASDTRWALPTPPKPAPVQTSTYK
                     AGTRVRHGLWGEGIVLDSKLQDGDEIVDVVFESVGIKRLAASLAKLTII"
     gene            complement(19670..20068)
                     /gene="mscL"
                     /locus_tag="PL000_08660"
     CDS             complement(19670..20068)
                     /gene="mscL"
                     /locus_tag="PL000_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HNC89011.1"
                     /translation="MLKEFKEFVMRGNVLDLAVAVIIGGAFGKIVGSLVNDILMPLIG
                     LVMGGINFSEKSLTVGAAVVKWGVFIQSVIDFLVVAFVIFLVVKAANSMKKTPPPAPA
                     AAPTTKECPHCFSTISIKATRCPNCTSELK"
     gene            20418..21407
                     /locus_tag="PL000_08665"
     CDS             20418..21407
                     /locus_tag="PL000_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89012.1"
                     /translation="MPDLLLDVRGLETQFKTPDGIVHAVNGVSFGLKEGETLGVVGES
                     GCGKSVTMHSVLGLIPSPPGKVTAGEAFFFGQDLLKMTHEEIRHVRGAQISMVFQDPM
                     TSLNPVLTIGRQLQDPLMLHTGMTKNQALERAAELLSLVGIPNAKDRLNDYPHQYSGG
                     MRQRVMIAMALSCSPQILIADEPTTALDVTIQAQIVDLVKRLREELGMSIVWITHDLG
                     VVAGIAQRVLVMYGGFIIEEASVAELFANPSHPYTLGLLGSLPRVDDTEHGRLFSIEG
                     HPPVLYKKPTACPFAPRCKWAMERCWKENPALEQIANEHRVACWVDTKTGRNR"
     gene            21407..22396
                     /locus_tag="PL000_08670"
     CDS             21407..22396
                     /locus_tag="PL000_08670"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNC89013.1"
                     /translation="MNSQNTNEVLLKVDDLKMHFPIYRGVFQRQVGAVHAVDGVSFDV
                     MRGETLGLVGESGCGKSTTGRTVLQLYKPTAGNVVFDGVNLVGLKNEEMRKTRRKIQM
                     IFQDPYASLNPRMTVADIISEPLMVHNVGTGKEIQERVAHLLELVNLNPAFATRYPHE
                     FSGGQRQRIGIARALALQPSFIVCDEPISALDVSIQAQVVNLLEELQEQFNLTYLFIA
                     HDLSMVRHISKRIAVMYLGVIMELAERDELYLKPLHPYTQALLSAVPIPDPVADSKRK
                     RIILEGDVPSPVNPPSGCRFRTRCPIADKVCAESRPEFREVTPGHFVACHLVK"
     gene            22510..24582
                     /locus_tag="PL000_08675"
     CDS             22510..24582
                     /locus_tag="PL000_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89014.1"
                     /translation="MRKMFALLSLLVIASMALSACGGTAPTAAPSTGDTTTEATEAPS
                     TDTGSEAPAAEFKSADPATLVVAESEVGIDTLDPALAYDTASGEIIQNTYETLVFYDG
                     EATDKFVPMLAESWELSDDGMTYTFKIRSGVKFHEGQDMTASDVAYSFQRGLLQGGTS
                     SPQWLLAEPFFGVGVDDVSVVVDPEGTLYDDRELLSAFDAAAVKAACEKVQAAIVADD
                     AAGTVTMTLAQPWGPFLSTIAQTWGSVMSKSWVVENGGWDGSCDTWQNFYGMASADDP
                     FSGIVNGTGPFTLEKFANGEEIILAKNPNYWGEPAKLDRVILKQVPEWGTRFAMMQAG
                     DADLAVVPAANRSQMDELVGIYQVFDLAANAYGPEQEVCGYDDTALAAAKFTACAAGE
                     TGNGGPFRLRIGRPAISMDVLLMNWNIATSEESPNPYIGSGALDGNGIPADFFSDVHI
                     RKAFAYCFDWDTLINDVFSGEAVQSYQLPLAGMPGYFPDTPHYTFDPAQCEAEFKLAD
                     VDKDGIAAGDDPEGDVWTTGFRVQMAYNQGNNTRQTITEILSGNLASVNELFLVETLG
                     LPWPAYLRAQRAKQIPIMTGGWLEDIHDPHNWYQPYTTGTYGGRQSLPDDVKAQFRDI
                     LERGVSETDPAKRAEIYKEANQLYYDLAVGIPLDVTTSHGFSQRWVKGVVLNPIFPGN
                     YFYTMYKD"
     gene            24730..25794
                     /locus_tag="PL000_08680"
     CDS             24730..25794
                     /locus_tag="PL000_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89015.1"
                     /translation="MTTYIIRRLLLVPVLLFGVTILIFAMLQFLSPVERTALYVRDFP
                     KNEKAIEGLIKRYGFDQPFYVQYWTWLVGKVDSTTGEREGGILFGNFGYSRSASQPVA
                     ELIQRRFPNTLDLTIWAVAPMILIGIWLGVQAAVHHNGFIDQAARAFSIVGTSFPSFV
                     FGLLMLLIFYANLHWFSPGRLSDEFNRVVESEAFRQYTTVLTIDALLNGRFDVFLDAL
                     RHMFLPILTLSYINWATFLRVTRGSMLETLRMEYVNTARAMGLPERDVIFKHAQPNAM
                     LPVLTLAGFSVVGLLGGVVITETVYNFPGIGSAAARAATSLDVVAMLGFALFNGFILI
                     VANLVVDVLYAFVDPRVRLS"
     gene            25808..26848
                     /locus_tag="PL000_08685"
     CDS             25808..26848
                     /locus_tag="PL000_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89016.1"
                     /translation="MTAATPSGDNTLLGENVATRKIGRVERFLGPENYRIITGLLKTP
                     ASIAGFFLIGFFIFVAIAAPVIMPPVTPNDPYKIPRDGFRSDPRPMGSAWTRNVPDIP
                     FWYTALTGKDKWVHIMGTSEGQYDIFYGMVWGTRTAFQTGLVVTFSTFLIGIIYGSVS
                     AYYGGVVDNIMMRIVDVLLILPGILAVLILAAVLTPKIGKSLIPAMAALIAFGWMGYS
                     RIIRGDILSVKERDYVLAARVIGVKDSRILFRHILPNAIFPTLVLASLAIGDVVLSFA
                     ALSFLGIGTEVGYADWGQVISFARNWITSLNTYWYIIVWPGVTLVLFVMGWNLVGDAL
                     RDVLDPRMRGKT"
     gene            26897..27550
                     /locus_tag="PL000_08690"
     CDS             26897..27550
                     /locus_tag="PL000_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC89017.1"
                     /translation="MALLFAGLFFDIDTVASQAVATPTIDRLAQPTLPADPTQADQGS
                     QVYWLSCLPCHGDRGQGLTDEFRQTYPVEDQNCWISGCHGKRPYDNGFTLPTAIPAIV
                     GPNTLQKFETAASLRSYIFAAMPYWKPASLTEEETWQVTAYILRESKRLPADMELNAS
                     NAASVLVGPPVATSTPQPGPDSVINLDEIQRPVLIVAVLVVALLLALRIFKTTVNKE"
     gene            27744..29816
                     /locus_tag="PL000_08695"
     CDS             27744..29816
                     /locus_tag="PL000_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89018.1"
                     /translation="MRKMFALLSLLVIASMALSACGGTAPTAAPSTGDTTTEATEAPS
                     TDTGSEAPAAEFKSADPATLVVAESEVGIDTLDPALAYDTASGEIIQNTYETLVFYDG
                     EATDKFVPMLAESWELSDDGMTYTFKIRSGVKFHEGQDMTASDVAYSFQRGLLQGGTS
                     SPQWLLAEPFFGVGVDDVSVVVDPEGTLYDDRELLSAFDAAAVKAACEKVQAAIVADD
                     AAGTVTMTLAQPWGPFLSTIAQTWGSVMSKSWVVENGGWDGSCDTWQNFYGMASADDP
                     FSGIVNGTGPFTLEKFANGEEIILAKNPNYWGEPAKLDRVILKQVPEWGTRFAMMQAG
                     DADLAVVPAANRSQMDELVGIYQVFDLAANAYGPEQEVCGYDDTALAAAKFTACAAGE
                     TGNGGPFRLRIGRPAISMDVLLMNWNIATSEESPNPYIGSGALDGNGIPADFFSDVHI
                     RKAFAYCFDWDTLINDVFSGEAVQSYQLPLAGMPGYFPDTPHYTFDPAQCEAEFKLAD
                     VDKDGIAAGDDPEGDVWTTGFRVQMAYNQGNNTRQTITEILSGNLASVNELFLVETLG
                     LPWPAYLRAQRAKQIPIMTGGWLEDIHDPHNWYQPYTTGTYGGRQSLPDDVKAQFRDI
                     LERGVSETDPAKRAEIYKEANQLYYDLAVGIPLEVATSHGFSQRWVQGIVFNPIFPGF
                     YYYTMYKD"
     gene            29925..31865
                     /locus_tag="PL000_08700"
     CDS             29925..31865
                     /locus_tag="PL000_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89019.1"
                     /translation="MPSSKANVKLFLFFLFLFIMACGFSSATPEPTAIPANTEIPLPT
                     ATPIPSSLTVCLGQEPNTLYPYGGPNTAARSVLAAVYDGPIDMVGYEYQPVILSQLPS
                     IENGDAQIVPTTVQPGGQAVDAEGNLVLLERGKKFRPAGCRGDDCIITYDGSTPLEMD
                     QMVVTFRLREGLTWADGTPMVSDDSVYAYDISRDSNAVANTYLIDRTYTYEATDPLTV
                     QWWGMPGFIDPTYFTNFWTPAPRHLWSQFPADQLPEIDIASRTPVGWGPYVIQEWIAG
                     DHITLTKNPYYSAAADGFPKFDTLSFRFIADPDTALSEMVAGRCDILDPSIRLDNHAS
                     LLLEMEKGEQAQVFFTPGMTIEWLGIGTTPSTYDDGYDTTYQNDRPDFFSDPHTRQGI
                     AYCLDRQSVVNNVLFGLTTIPATYLPVGHPLYDNNLQPLPFDVATGVSLLEQAGWKDT
                     DGDPTTPRQAVNVKNVKAGTPLQLNYYTTTATQRRQVVEILQNSLAQCGIGLNVQYFS
                     QNDLYASGPDGALFGRRFDLIEYAMGVGSIEPPCDWFTSEEIPTSENSWVGTNVTGYR
                     NEAYDTACRAAQLSLPDEQIYVDSYRQTQVLFSSELPAIPLYYRLRVAAARPGLCHFE
                     LDPTANPLWNIEAIATGEACQN"
     gene            31865..>33783
                     /locus_tag="PL000_08705"
     CDS             31865..>33783
                     /locus_tag="PL000_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89020.1"
                     /translation="MRRLFHIGLFLTLLLVSCSPQPARVAEPTSIPTPTLAPVVAHAP
                     EIRFALVGEPQTVNVWQLFDTPGASYADYALRADSWPRLYHLLPPEFTFEPLAASGLP
                     TAFTQENDFYVAAVKLRTDLKWTDGSAFTAEDVAFTVNAALAFELGFDWSEFYPSDDL
                     HHAEALDASTVKYYFKQEPNVGAWQYGALQGPVVQKAFWEGAVNAAANSLPTETLRTE
                     IAKTQADLETVKRDVAELSEQVLALILSGKQNRILDSDLKRKQNEQIFVQSNLDRLLE
                     EYAANIAAAQSALYASSDEKEPLLGSWIPLGMEDGQWVNEANLEFPFIQPGFDRAVYK
                     IFESETEALTAFENGEVDAILTPAGISSNIPGMVYSPTYSARFLVFNPLKTQFADPAF
                     HSALSCMLDRNTLSSDRLQNQAFPFDAFVLSPQWHDPAAQDPCTGLDEATRIGQAVKF
                     MKDAGYFWAKEPDANGAGQGLRYSNGDLFPTAVLLSPTKDEDPLRYAAANYVSERAEY
                     LGVSITVQTASMDDLIYAVYSSQQYDLAITGWRLSEYPAYLCEMFDGRSQHLYTSDRF
                     QSACDALKGESDLERARQGFRQIESALMMELPFIPLFTVARADLYQKIAYPAEKVLNS
                     WGGLYGAPSFAVPLP"
BASE COUNT         7586 a         8875 c         8674 g         8448 t
ORIGIN      
        1 ttgcccttgt ggacgaagtt cacgctcttg cgcttgttct ccagcgccca ctgaatggcg
       61 gcgcgggcaa ggcgttcggt gccttcttca gagaccggct tgaagccgat gcccgaagtt
      121 tcagggaagc gcatcttggc gtattccttg gggaaggctt ccttgaacat ttccttgaag
      181 cgcttgttat cttcggtgcc gtgttggaat tcaataccag cataaatatc ttcggtgttt
      241 tcgcggaaga tgaccatatc cacgaattcg gggtggttca ctggcgaggg cacgccctta
      301 taccagcgca ccgggcgcag acaaacgtac aggtcgagca gtttgcgcag cgccacattc
      361 aaagagcgga taccgccgcc gatgggggtg gtgagaggtc ccttgatacc gactaaaaat
      421 tccttgaagg cctcggtggt ttcatcgggc agccagctct ggaacatctt gaacgatttc
      481 tcgcccgcgt acacttccat ccagttgatc ttgcgtttgc cgccgtacgc cttttctaca
      541 gccgcatcga acacacgcac cgaagcgcgc cagatgtcgc ggcctgtgcc gtcaccctca
      601 acgaagggaa tgattggatt gtcaggtacg ttcaacttgc catcttgaat ggaaatcttt
      661 gccccacctt cagggacttt tacatttgtg tatgccatgt cttcctcttt tggtttgtga
      721 aaatttatgc ggattataac atgcggactt tttccgcttt cagtgacgat tgtcacgaca
      781 tttccgcatc caaagcggac ctgcggccgg ggtgcccacc caggcagaat cgtcgccgaa
      841 gatttgctgc gataaaaaat gcggggagaa actgcccgta aaacagaatc cgcttcaggt
      901 taaggctgaa acggattctc atccaaaaaa gcaggcggtt tggcctgact caggcttaca
      961 tggatttggc gcgggctgcc atttgttcgc ggaggtcgtg tgaatctttg tcgatggtga
     1021 agatcgcgcc gaggtagaag acgaaacaca aaagccaggc tgctgtgcaa atgatcagga
     1081 tggcggtttt cagatcgtag agatctgcga tgtaccctgt gaggatgggg gcaaaggctg
     1141 cccccgcatt ttcaatgaag tattccacag cctgtgcagt ggagcggacc tcgggtacgg
     1201 tgacatcgta aaccgtggcg atcacatttg ccgatgaaag cggcataaaa acggcggtga
     1261 ggcacatcaa aatgaagaat tgatttttgg cgtctacggg tgtggtgatg gcaagataca
     1321 tgaacaatgc gccaagcaaa accccgatgc tggaaacaac gatgcggcct ttgttcgagc
     1381 gtttgaaggc catgtcgccg agcgcacctc cgatgaaata tccgctggcg aggatgagga
     1441 tgacgggtgc cattgtgaag agaatgccgt ttgcatcgta gccgcgttct ttttcaagat
     1501 aggcgaagaa aaagaaggtg atcacattcc acgggaaaac gcccgcaaag ccctgcagga
     1561 aaacgaacca catggtcttc tttttgaaga tctctctggc ttcggtccat gagaatttga
     1621 aggtctgcat ttcgggcata ttctcaaatt caggctcggc ctgtccgcgc ggcatttctc
     1681 tcacgccgaa atagatcaac acggcgatga ccaaccccaa cgagcctgtg atgtagaaaa
     1741 cactgcgcca tccgccaatg gcaggtgcaa ccatcaacgc caggatcatc ccaaccagat
     1801 agccgatggg ctgggccaat tgcaggatgc cgtagatctt tccgcgcagg ttggggccga
     1861 aataatcggc gatcagggtg tacaggccgg ggtaggatga atcatcaatg ccggtggaag
     1921 cgcgcgtggt taaaaatccg ccaaaggttt ttacgatggc attaagccag gtggttgcac
     1981 cccaaatgaa ggaggccagt gaaagcaatt ttgtgcgtga atagcggtcg tataaatatc
     2041 cccagatcgg atacaggatc gtggcaacga tcagagcccc tgaattgatc aaaccccatt
     2101 gttggttgtt gagattaaag tccttgctga tatccacttg catggaaccg atcatcaatt
     2161 tgtcggtctg gtgcaggagc atgaagaaaa agaaaacaac aacgacgaac cagcgatacc
     2221 gtgaattctc ttttgacatg aatacctcgc ggcgatagga tgccctagta tccagtgtca
     2281 ggtgtcaggt gtcaagaggc aggtttcagt tttcaagcgt gtcttctgaa atgatctcct
     2341 gcgcaagttt gttgagttca tcggcatctt tcaccttggc ggcatcgaag tattgtgcca
     2401 gcaggtccag cggactcaaa ctgctcacca cctgaccttc gggaatgcgc acccgcgctt
     2461 cgctttgcgg tctttttacc agatggaact caaaggcatc ctcagtatat tttcgcaaag
     2521 ccgtttcatc gatcaatgtg tcccactctc ttggatattc caccgacagg cggacaatgg
     2581 cctcagacat ttccttcagg ctgggcaatg cgtctttaag agcctcggtc acgttctcgt
     2641 tggacctgag aactgttcgg cggtcgatga attttctcgc ccctgtgagt tgaacccact
     2701 ccacctgcgt ttcttttccg ctttcgatct cggcaatgac aaagaaacgg tcttcttttg
     2761 cttcgccaaa atccacacgt tcaatggagc ccgggtaaat gacaggcggc tgatggcctt
     2821 cgttcacatc ctgcggtttg tggatgtgac ccatcgccac gtaattgaat ctctggttct
     2881 tcaccaaact gcctgaaagc acgaggtcat tgccaagcat cacaagccgc tcgccgccga
     2941 actttgcgcc ttcaatggag gcatgggctg tgaaaatgat cggcaggttt tcattggctt
     3001 cctcaatcca accatccaca aggcttgcga tccgctcttc gatgttcgag tagatctgct
     3061 cggtttctgt tccgctcatt tcgaggttgg ccatcaaccc cgagcgtgtg acccacggca
     3121 tggcaatgac ctgcatgggt agttcccaca ggtcttcagg tttgagaaaa ctgggctggt
     3181 ccagcacttt tacgaacgga acttgcaggg tcttgaattc ctgcaatgca tgggcgcgcc
     3241 ccaccgccgg ggaaatatca tggtttccga tcaacaaaag ggttgggatt tgcgcctgtg
     3301 ataagcgcat gatgcgcctg ccccattcgc gttgaaaggt tggcgcagga gaacggtctt
     3361 tgtatgcatc gcccgcaaag atgaccaggt ccactttttg agagatggcc gtatccacga
     3421 tcgtatcgag tgacttgagg aaatcaagca cacgcatggg caggcctgtc tgcgggtcgt
     3481 ggcggccata gttggccatg tcaatatgtg cgtcggcgaa atgtaagact ctcattcaac
     3541 tccatctaat atgtgtttac aggttggaag gtttgcaggt tacaggttga accttccaac
     3601 ctgtaacctt gccaacttgt aacccattac ggctgtagcc cgaggtcttg aagcgcctgt
     3661 acggcgggcg gccagttcac gtggatccgc aatgcggcgc gataatcatc tatggcggcc
     3721 tgggtattgc cgctcatata gtaggcacgt ccgcgccaaa gcagggactc ttcgagtgtg
     3781 cggccttttt tgttggcggg gtcgaagagt gttgtgtcgg caaggctgat gacatcggca
     3841 tagcgggcgg aataaaagta ggcgaaatag ggtcccgtct gataccacat gatgcggtag
     3901 ggacgttcct gttctttttc atcccagttg gcgtaagtgg aaaatgcctg atcgtaagcc
     3961 gcggcggcat ccacatattg ttttaggagg acatggctgg tgcctttgtt gaaccaggca
     4021 aagaacagat tgttatcgtt gatgctttga atgtcctttt cggcgagttc aagcgcatgt
     4081 tgggccgccc aattttcgtc cacccatggg cccaacaggg ttaacacttc ggcttcgcgc
     4141 tccgcgggat acaccacgat gaattgataa ttgaaagcac gccagccttc cagataggtc
     4201 gcataatcgc tgagaagatt tttaccgggt ttggtgtagg catcttgtac gatgaatccg
     4261 ccgcgggcat catcgtagcc ggtggtgaag aggtaatgcc cgagccagct gatcttgccg
     4321 gtggtgtcgt gttcgtaata acctttttct acaacaacgg ggaaacccgc cgccagcagg
     4381 cgtttgacga ggttttcgtc gccgccgtgg cgcatgagcg cacggtattc ggtgttctcg
     4441 ttgacgaaat ccaccagctc gtagggcatg acgtttttat cgtctttgct gcggtcaata
     4501 aagttgactt ttgtatcgcc cgagcccggt ttgacgatct tggctacgtc atcgcggttg
     4561 cctttccaac cccagaagtt aagcgccatg gtcaggttgg cggggccgca atagttccaa
     4621 cgttcgtgct ggtctacgta cacaacccct tccaaattga ccgcttccgg caagggtgtg
     4681 gatgtcgccg tgggtctggc tgtcggccct gtagtgggag tgacggtcgg tgtatattgc
     4741 ggggtgaggg tcagcatgta ttcagcgcgg gctgtggcaa tggctgtttc gatggtgagc
     4801 ggggaagtct caccactggg ttcgaagacc acttcatcgg gcgggttgaa gaaataaata
     4861 acctgcacac gcagggcatc cacacgccag gcgaggcggc tgtgaacggg cggcaggtaa
     4921 taaatgccaa cggcaagcag cagcacgatg ggaatcaacc agtatttatt aaatttggac
     4981 ttcattttca tgagatggat tataaagcaa attgaatttg caatcttgca tcgtttcggc
     5041 gtgttttgcg taaaacaaag cggaggtact tatgactact caaacaaaac aacacatccc
     5101 ctggtatttg tggccgtttg cggcattgtg gaaactgctt gccgtgatcg tggaattgac
     5161 aggccgtttc gttgcgatgg tgcttggcct ggtgctgatc ctcgttggcg gtatcgtcag
     5221 cctgacggtg atcggtgcga tcattgggat tccgctggca attatcggtc tgctgttgct
     5281 gctgcgcgga attttttaga gaaagagacc ccaaagattt tcaaaacctt tggggtcttt
     5341 gtattaaggc gtatccactt ccggttgtgg attctttttg ttgagcaggg ccaggctgcg
     5401 ggttgaaaaa gaggcgacgg cttttcgaat cgggggaatg cggaggacca gaaaaattcc
     5461 gatgatgagt ccgtagatca gtggtttaag gatttctccc tgtaataaaa gcaggtcgcc
     5521 ttttttgctc caaacataat gaaggaagac cagcggggcg atgaggtaga cgagctggtg
     5581 caggcgtttc cagtttttgc cgaggcgttt tttccagatg tcgaacgagg tgagcgccag
     5641 cggaatgagc aggatgaaag cgataacacc gacgatcaga cgcggctttt cgatgacttc
     5701 tttgataacg ctgctccagg ccagcccgaa gtcaaggtca acgaagatga tcatgtgaac
     5761 ggtggcatat agaaaggcgt acacaccgag tgcgcggcgg cgtttgatcg gttcgctcca
     5821 tttgaagatg gtgttgatgg gagtgcatag cagtgacagc acaagtaatg taatggcgtg
     5881 cctgcctgtg cgctgttcaa ggtcttgaat ggggttgggg gaaagattac ctgtgaaata
     5941 atccagcagc aaaagggcaa gccctgacca tgcatacagg tgaacggcga tctgtaacgg
     6001 tgtgtagggg aattttttca tgaagtgtct accgaagata aacggagatg ctgttgcgat
     6061 ctccgtttat ctctgttcat ctctggttag taattcacgc tcaagtccat gccgtcatag
     6121 aggtgcgcaa cctgttcgcc atagccattg aagggcaggg tgggcctgcg gttgagttcg
     6181 ccaatgcggc gctcggaggc ttgtgaccag cgcggatggt ccacatcagg atttacgttg
     6241 gcataaaacc cgtattcgtt cgatgcgatg ttgctccaaa gtgtggatgg ttcttcggcc
     6301 accagttcga tcttcacgat ggatttgatg gatttgaatc catacttcca tggcactacc
     6361 aggcggatcg gcgcaccgtt ctgtgccacg ttgggttcac catagagacc tgtggcaagc
     6421 agggtgaggt cgttcatggc ttcatcgagc cgcagtcctt cctgataggg ccagggatag
     6481 aatgggcttt tttgtccggg catttcttcg gggcgatata cggtttcaaa gcggacatat
     6541 tttgcatcgg aagtcggctc taccatttcg agcaggctgg ccagcgtaaa acccgtccac
     6601 ggaatgacca tgctccaggc ttccacgcaa cgcaggcgat aaatgcgttc ttcctgcggg
     6661 aataacttca acaggtcttc gatgccaaac gtcttggggt tgttaaccag gccgcccact
     6721 tccacggtcc atggggccag gttgaaacct cttgcttcat cggccacctg ttgtttgtcg
     6781 acggtgaact catagtagtt gttgtaattg gtgatatcgg tataggaatt gaccgggtca
     6841 cccaattcat cggtcgcatt tgatgttggg acgggtgcat tgtcctcggc agctccggcc
     6901 acggaaggag cgcaggcggt caacaaagcc gcccctgtgg tgagtgtggc ggctttcata
     6961 aaatcacgac gtgaaagata cacgctcttc ggcgtgatct cggaagagcg gatcgggacg
     7021 gttttaaata aatcggtcat ggtttaaatc tcctcataag ttggcgcgga taattcatgg
     7081 aaatcaaatt caatacgagt gttcaggtta actcgtatga tgtccccatt ctaaaaaaac
     7141 tttcttatat gcagatgata gtttgcttag gaattgtgcg gctagtctaa tttccgaatc
     7201 gttgggtgca gcaatggcag caggctgacc aacgctgtca ccacacctgc ggcaataaaa
     7261 acggatgaca caccgatggt gtcgatgagc cagccggtaa aggcgtatcc aatggggagc
     7321 aacacgaacg acccaagcga atccacgctg gagacacggc ccattttttc gggaggcact
     7381 ttttcctgca tgaggttggt ccagatgagg ccgtcaattt cgagggctgc accgttgatc
     7441 aatgctgcaa tgaccagcag ataaagcggg agaccgaaac cgaacacgcc caacccgatc
     7501 cctgcaatgg cgccgcaaac gtagagcagg attccgcgat atcgtatctt tcgaaaaccg
     7561 cccatgacca ggctggcgat tgcataccca atggggaaaa tggaataaat aaagcccagc
     7621 attttttcgt cgccgcccaa tttttcctga acgagaaaag gcatggcaat gctgtagggg
     7681 cctgccaggg tgatgtttgc aaatgcgaag acaatgatcg aaacccacaa tacaggggtg
     7741 gaaaagatgg tgttgaagcc gtcgcgcagg tcgtctttaa aatgctggag ggtaaaaccg
     7801 ctttcttctg aagaagcagg caccgggagg ttaagaagcg gaatgaccgt caatgcgccg
     7861 atgaaaaagg acagggcatt gaatccaaat gcgacggcgg ttccaccgaa accggcgatc
     7921 aatccaccga gggccgggcc tgccacgcgc ccgaattgca tgctcaaact gttgattgaa
     7981 tttgcgctgg gcaggtcttc ttcggcaacg atctgttgaa tgagcgcgtt ataggcgggg
     8041 agaaagaagg catcggcaaa cccgaagaga atggcaatga catagatctc ccaaagaaca
     8101 agctggtctg tgaaggccag ccatgcccca atgaccatga tgaaacaccg cgccgtgtct
     8161 gaaatgaaca acaacaagcc gcgccgcatc cgatccacca ccacgccgcc gaccagtacg
     8221 aaagccacca tgggtaatgt gctgaagaac aaaaccgtgc ccatggcggt ggcagagcct
     8281 gtcttttcca gcacccacca ggccagcgcg atctgaaaca gaaaatcacc gatccttgaa
     8341 aaggtctggc ctaaaagcag ggcggcaaat tttttatctc ttaaagcgcg gaaagtggat
     8401 ttcatttcac acctctgatt ctaaaattga tgactcgcct tgtatttaat aaaagcaatc
     8461 tttcttcttg gcattttggc ggcagggaag aaagattttc aaagaaaact atggacgggc
     8521 acggtcactt gaatgattcg accgctgatt gtatgttgcg atagttggga atatttaaaa
     8581 ggaaccccgt aatgccaagg atgttattca cttgagatgg ggcattgctg atggccacat
     8641 gcagccgctt catgcgttct tcggtgggtg taaacagttt gtagaccttg tgcatggccc
     8701 gcatccctgc gctggtgagc gtctccaccc cggcaaggtc gagcaccaga tgacgggcgc
     8761 cttcactgta tgcatcgcgg gcggcggcga gcagggtttc ctctcctctt gcatcgagcc
     8821 agccttgcag atggaacaca gtgatgggaa tgtgcgcctg aattttttcc tgggtgaccg
     8881 ttaatgtggt ttccatgatg tttaagatta tacatctgat gcaagggagt gcgtgcctct
     8941 cccatgacct ttggattatc cctgttccac cggcaatcct gaagatgggg atccccactc
     9001 ggcccatgaa ccatcataga cggaaatatt ttggtagcct gccagttctg ctgcaagcgc
     9061 atcaatgcag gctgtgacac ccgatccgca gctaaagatg agtttctgtt ccttttttac
     9121 caatttcgaa aaaatggatt cgagttgcgc gggcggctgc atggatccat tcactaccac
     9181 atcggcaaat ggcaggttga gcgagttggg catgcgcccg ccccggagac ctgcacgcgg
     9241 ctcgggctcc acccctttga agcgccccgg tgagcgggca tccaacacgg caaaagcgga
     9301 atcggcaagc gcctcacgta catgcgtaga atcgcagaac aacccggcct gtggatgtgc
     9361 cacgaaatct ccgcgccgac tggctttggt ttcagcctgt tctccgcatg ggagcccggc
     9421 ttgtttccat gctggcaggc cgccattgag tacggctacc tgctcatgtc ccatggcgcg
     9481 gaacatccac catgcccgtg gactggagta cacgcccaca tagtcataga ccacgatggc
     9541 gctgttctgg ttgatgccca gtttttgcat ttcctctgtg aagaattctg ctgtgggcat
     9601 catgtgcggc aggtcagtgt tcttttcgcg gatgtcgttg tcaaaatcga atcgcaatgc
     9661 acctttgata cagcccgcct gatctgaaga agcgtttccc accggggtga ccggtttcat
     9721 gcttgcatcc aaaatgatga gattttccgc ctcgagattt tcagccagcc attgcacaga
     9781 gaccaatgaa gatgtgattt ttacgtttgc catgatgcgc cacctgtttt gtcggttttg
     9841 aaaattatac gatacgttat gagtttaaat aaaaatggga caggccgttt tacgaggcct
     9901 gtcccactga tcactactta ctattcacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnaa tgggacaggc cgttttacga ggcctgtccc actgatcact acttactatt
    10081 caccaaatct atccgtgcct gctcatgaac ctttccatgc ggcgcagggc ttcttcgatc
    10141 tggctgtatt cggtggcgta acaagcgcgg acaaatccct ctccacccgg gccgaaggca
    10201 ttgcccggca ccacggccac accctcttca cgcagcaggg cttcggcgaa ggtttcatcg
    10261 tccatgccgg tggcctggat gttagggaat gtatagaaag caccacgcgg ctcaaaggtc
    10321 gaaagaccga ggcggttcaa tccatccacc agcaggcgac ggcggcggtt gtattccgtc
    10381 accatttcct gcacatgggg ctctccgttc ttcaaggctt caatggcggc atcctgcgca
    10441 gtggtcggcg cagacatgat cgtatattga tggacacgca ccatgccttg aatgatatcg
    10501 gcaggcccgc aggcgtagcc gatgcgccag cctgtcatgg cgtaggcttt ggagaacccg
    10561 ccgagcagca ccgtgcggcg gcgcaggctt tcgtccaatg cagggaagca gacgtgttcg
    10621 aagtcgtaca caaggcggtc gtaaatttca tcggagacca cgatcaggtt gtattgttct
    10681 gcgatcctgc cgatctccac catcacttcg cgcggggcca cggccccggt ggggttggat
    10741 ggataaccaa taaagatgac cttcgtgcgc ggggtgatgg ccttgcggat atcgtccggg
    10801 tcaatggtga agttgttttc gagccgcgct ggaatttcga tcggcacgcc gccagccagg
    10861 atcacttccg cctgatagga aacaaaacat ggggtcggaa tgatgacctc atcgcccggt
    10921 tccagaatgg cggtgaaggt gagatacaac gcctccgaaa cacccacagt cgccacaacc
    10981 tcggaaagcg gatcgtattt tacgttgtag agtttttgca gattctccgc aactccctga
    11041 cgtaattcaa ccttgccgtg attggacgtg taatgcgttt caccattttg cagggaacga
    11101 atccccgcat cgaggatcgg tttgggtgtg gtgaaatcag gttcaccgat tcccaaagag
    11161 atcacatcct tcatggtcgc aacgatatcg aaaaattttc taatgccgct gggctttaat
    11221 tcagccacac gtttcgacaa ccgattcaca ttgcttattt cacttgctgc ctgcattgag
    11281 cctccttata tgggttgaac gtgccagtcg tctgagattt cctggtacgt taattcaaat
    11341 gtaaaaccgt cggtcgtttt cacgcaaaaa cctttttcac cggggccgcg ccagcgcgaa
    11401 atgatctctg caatttcata acgggctccc cgccataaaa gagagagcgg cctttccgca
    11461 tattcagtgt cggaacgaca ttccacagct tccatggtta cagttcaacc gagcttgtat
    11521 cgccgatgtt caactgcatg acgtgaccat cgcctcgcgc cttgaccctg gcctgcctgc
    11581 cgatcaacga gcgactcagc gccgcatcct tgatctcaca acctgcttca atgatgctct
    11641 ccgcgatctg gcagttctct attttgcaat ttgcgccgat cgaagcatac ggcccaatgg
    11701 tggaattgga aatctccgcg cttggatcga tgaatgaagg ctcaacaatg ttcacgtttg
    11761 aacgttttaa cgcttcaacg tttgaacgtt caaacgcccc agcgctccaa ctttccaata
    11821 aggttttgtt cgtatccaaa gtcgcttcga tggttcccgt atccagccag gtgctgatct
    11881 gattcgtgcg gacctttgct ccgtgttcga tcatcactgt gatcgtgtcg gtgaggaagt
    11941 attcgccctt gagcatgatt ccgcgctgca tttgttcatc gatggcggca aggagtttct
    12001 cggcgctttt gaagtaataa cacccaacta caacaaggtt gttgtccatg ctttgcggct
    12061 tctcaatgaa gcgagtcacc cagccttcgt tgtccaactc cgccacccca aagcggcgcg
    12121 gatcaggcac cggcatcacc caggccacgc catcggctgc ctcgttcgcg aggaaggaga
    12181 aatctgtctc cattaaagtg tctgaaaaac agaccatcat cgggccgcgc aaatattcgc
    12241 gtgaaagcgc caacgcgtgt gattgtccct tcatttcatg ctgcaccaca tagtgcgctt
    12301 tgaggttcgg gtatttttct ttgataaaag cagggacttg cagttcgccg agatatggcc
    12361 ccacaatgaa aatgtattcc acattttcag gttccggcag agtcttgaac atatccatca
    12421 aatgttcaag tgacgttttg cccgccacac ttaccagcgg cttcggcttg ctccacgtat
    12481 gcgggcgcat ccgcgttccc cagccagcca tgggaatgac gatctttagg gtttcagcca
    12541 agagatttct cctgtgaatg atttcaaggg tattttacca atgaatacct gccagcgata
    12601 taaaaaaact cccgtcctat ctatcgtagt gcgggatatt atcccgcact acgcctcact
    12661 atcaagagcc agttccttca gcggcggttg atgacaactc taagtttgcg gttacaaaaa
    12721 agagacagtc acctcataag tgactgtctc tttaattcac gcaccgatct acaaagaatc
    12781 ctggttattt attcttccat ttgctcaagt ttttccatct gctcttcgat cagatccaca
    12841 tacaccttga acgttttggc atcgagtgag aattttgctc ccgctgtttc aaataactca
    12901 ggcagtgaaa ccgtggaacc gagcgccagc gccttgcggt aattttccac agctttcttc
    12961 tggtctttga gtgcattggc ccacacctgc accgcgccca attgagcgag accatattca
    13021 atgtaataga aaggagtcgt gtggatgtgt ccctgacgat gccagtaagt cgttttggca
    13081 tcctcgagtc cgctgtaatc cacgccgggg aggaagcgat cccacaattc cccccatttt
    13141 ttatcacact gcaccgggtc tgaactgatc tgcgggtttt catagatcca gtgttggaag
    13201 gcatccacca atgccatgta cggccagaag gtaacgatcc cttccaaatg ttcgaagcgg
    13261 gcgcgggcag cttcgccctt ggtgtagaaa cctccatgtt ccctggtgat gtagggagaa
    13321 gccagcaatt ccatcgccat ggatgccact tctgcaaatt cagcagggac ataattctcg
    13381 gcgcgctcat ggaaatatgg aacgaccgcc gcttcgaatg catgaaaggc atgtccgcct
    13441 tcatgcagta tggtgtccac atccatcggc gtgccggtgt tgctcataaa gataaaaggc
    13501 aattggctga ccccatagct caggttgtat gccccgggcg ccttgttctt acggctttca
    13561 aggtcgagca ggtcgttatc catcatcact tgaaagtatt cgccaaactt cgggtccacc
    13621 ttggtaaata catccttcga cctggacttg aattcttcga tggtttgata tggcttgagt
    13681 tgaccgcgat tcgatgtatc caccatctgg tcccacggac gcagcgtttc aagcccaagc
    13741 ttcttcctgc gtttttcata gaggcgggtc gccgcaggga caacaacctc ttcgatcgca
    13801 tcatgaaagg tcttgcaatc ggcgggtgaa taatcgaagc ggaatctttg cgcccacata
    13861 taatcacgat agttggacat accggcattt tttgtgatct tgagccgtaa gtccatgtac
    13921 tttttccaca ggctgtggat cgcttcgcgg ttctgcacac gcacctccgc caccagtttc
    13981 catgcctttt cgcggaccgc acgatccttg tcttgataca gcgggaacat ttgcgcgatg
    14041 gtgcgttcct ccccttccca catcatggtc tgtgcgccga ggatcttgtc gtattccccg
    14101 ttcagttttt gttcctcggt cagcaattca aggttggcct gcctgaagat atccgcttca
    14161 gcgcgcattt tcttcagacc cattttgaag ttttcaggct gcaaaccgct ggcgagcagc
    14221 ttttccttca gcttctgctc ttctgtgcgc gcattgggct ggatggattc gataaagtga
    14281 ttgaaattct tctcgatctc ttcatcggtc gtgtattgcg tggtcgcgac aaaacggcgc
    14341 gtgtattgct catccaatcg gtctgccaag gccgaccaat cggaaagcca ctcgcctatg
    14401 ttggcggcgg tcaattcgac agattgcaag gctttgaaat gtggttcgta atcaggccat
    14461 tccattgcaa ggatggcttc cggtgtagag ggtaaattct taagcatgag aaaactcctt
    14521 ttacttgtaa acgataaatc ccaataccgc cccggctatt accagccaaa ccgggttgat
    14581 ctcggtcaat gtcaacagtg taaacgcagc gatcgcaata cccaccttgt cccaattttg
    14641 taccaaccct tttgcccaac ccaccttgct tacactgaaa acagaatacg ccatcgtgta
    14701 ggccgtccac agcaacaacc ccaccacaat gggtcgcacg gccttcaaca tcgccgccac
    14761 agcagggctg tctttgatgc tgaagaaata gatcaccatc atcaacatca acaacgtggt
    14821 cggaaatacc gttcccattg tcgcagagac cgccccccac aaccccgaca ttttatagcc
    14881 cacataagcc gaaacctgcg gcgcaattgg ccccggcagg gcgtttccca ccgccactgc
    14941 atctgcgaat tcttccgtcg tggcccagcc tgccgtcacc acctcgcgct gcatcaaggc
    15001 cagtgaagcc ggtccgccgc cccatgagaa caaagccaca cggctgaaca acacgaaaat
    15061 ttcccagagc aacttcaatt ccatttcacc actctccaaa ctgcgggttg ggtttgcccg
    15121 ctttgtattc ttccagtctg cgcacatgcc cgggccataa tccttcgggc aatgcattca
    15181 aaggaaacga ctgaatttca gcgatctcac tgtcgggttg gcctgtgatc ttgaaatctt
    15241 cgcagacaaa aacaagattg tgatcggtct tccaatcctt gaagctggtg tacgcaccca
    15301 tcaaacggat ctccccgagt tccgcgcctg tttcttcata ggcttcgcgg cgtgcagctt
    15361 gatccaatgt ttcgccgcgt ttgagtccgc cgccgggcat gaaccagccc ggtagatacg
    15421 tctgccgcac aagcgtcact tttgaatcct ggatcatcat cacacgcaca cccatgcgca
    15481 caggccgaaa caaaaaacaa aagatccgat agccaaggaa tagaaaacgg atatacatgt
    15541 taggcatgtc ccaaaatcgg atgcggctgg tacaactctt cgagcaattg gatttcatcc
    15601 gctgtgagtt tgatctccaa tgcggcaatg gcctgatcca aatgctcgat cttgctcgac
    15661 ccgataatgg gtgcggtgat atggggtttg ttcaacaccc aggccaatgc cacctgtgaa
    15721 gccgatgcat tgtgactctt cgccactccc gccgcacgtt cggcaatgac aaaatcctcc
    15781 tcgcggaaat acaactcgtt cgcaaatgga tcgctttgtg cgcgtgtcgt ttcgccgcct
    15841 ccgccgcgtt tgcggttgcc tgcaaaaaat ccgcgtgcca ttgggctcca cggaatcaac
    15901 ccaattccct gatccttgca caacgggatc atctcacgct cttcctcgcg atatacaagg
    15961 ttatagtgat tctgcatcga aacgaatttt gaccagccat tcatctcgga aacatgcagc
    16021 gccttcataa attgccacgc aaacatcgaa gatgcgccaa tgtaccgcgc cttccccgca
    16081 cggacaacat cgttcaatgc ttccattgtc tcttcgatgg gggtgttgta atcccagcgg
    16141 tggatctggt acaaatccac atattccatt tgcaggcgtt tcagggattt gtcgatggag
    16201 tccatgatgt gtttgcgtga gagaccgcgg tcgttcacgt cctcgctcat ggtggagaac
    16261 accttcgtgg caacgatcac attttcgcgt tttacaccaa agtgacgaag tacattcccc
    16321 gtaatttttt cgctttcacc cagcgaataa acatctgccg tgtcgaaaaa attgattccc
    16381 gcatccaatg cacgttttac gaatggtttg gcctgttctt catccaacat ccactcgcgc
    16441 cattgcttcg agccgtaggt catcatcccc aaacacagcc gcgagacttt caatccagtt
    16501 ttgccgaggt tggtgtattg cattgtgaat ctccttgatg ttggatggtg gattttatgc
    16561 tcccaccgcc gtcctcggcg gtggagggga tagccattca tttatatctg taacagggat
    16621 caaaggcttt ctaccattgt cataatgaca ggcgctggaa aaagcgtaat ctgcccggtc
    16681 ttcgacaagt gtccagcctt tggctatggg gttttggtga atatactcga ttttctgcca
    16741 taaaaattct gttgagtaaa cgttcttggc ttgaatatcc tgccagatgc tatgttcgtg
    16801 tctttcgtcc ctgcgttttt gatgaaacac gtttaacagt tccggcttgt tttcggcttt
    16861 tagcattttg atgatctcat gcgctgtgaa agaaccaaat tgctgaagga tgtttccaat
    16921 tgaatcggat tctggcttga taattgcatg tatgtgagaa ggcatgatta caaacgcaaa
    16981 caacaaaatt cgtttttgtt cctgcatcca tgccagggaa tccattggaa tttttgcata
    17041 ttcaggcatt gcaaatagat gcttccattc tatgacggat gcggttatga aatataagtg
    17101 ggcagggtca tgtgaggaag aaaaggtcat gaagattgct tcctgccacc ccgcgccggn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggtcatgtga ggaagaaaag
    17281 gtcatgaaga ttgcttcctg ccaccccgcg ccggggacgg cgctgtgagc acatttggat
    17341 ggtggatagt ggttggtaaa ccgttttttg taccgtccac tttccagtgt atttatatga
    17401 ttgtcagctt tgcgaggctt gccgccaatc gtttgatgcc taccgattca aagaccacat
    17461 ccacgatctc gtcgccatct tgtaatttgc tatcgagtac gatgccctcg ccccacaaac
    17521 catgacggac ccttgtccca gctttgtagg tgctggtttg aacaggagca ggtttgggcg
    17581 gagtcggaag cgcccatctt gtatcggagg cagacgtgtc ggggcgtccg tataatccgc
    17641 gtgaggtggt gggtttgccg gtgcgtgttt tcccaaccag caattcggcg ggcaggtctt
    17701 cgagaaaacg tgagggataa gtctcttccg acatgccgcg tccaccgcgt tgaatggcgc
    17761 gcaacaggta gagtttatct tttgcgcggg tgatgccgac gtaaaagagg cggcgttcct
    17821 cttccatttg ttcaggttca tcgaaagagc ggctgtgtgg aatgatgccg tcgtcgaggc
    17881 caacaatgaa gaccgcgccg aactcaagtc cttttgcggc atgcagggtg agcagggtgg
    17941 gcgcgttcat gtctgtgatc gtgtcttgat cggcgacaag cgcgatgttt tcgaggaatt
    18001 catcaagcgt gcgtgtggaa tattcaagcg cgaggcgttt gagttcctgc acgttttccc
    18061 aacggtcttt gctttcatca gaatcatctt cgacgatgta ttctttgtag ttgatatctt
    18121 taacgatctt ttcaaagagt tcggcggggg tggcggtggc cgccatggcg cgccaggtgg
    18181 cgagcatccc gccgaaatca gcgaggggta gtgcggcacg gcctgtgaag gatttataga
    18241 aaggcgagtc actgccgcgc gcaagatcaa gcaggatcgc accgggtgat gtgttgtttt
    18301 ggcgggcggt taagtgaagg gtggtcagcg ttttttcacc gatgccgcgc ggcggtacat
    18361 tgataatacg gtcgaggctg gcttcatcgg caaggttgtg gataaggcgt atgaaggcga
    18421 tcacatcttt aacttcgcgg cgtccataaa atctttgcgc gccgacgagc ttgtaaggaa
    18481 ggcgtgcctg caggaacgct tcttcaagca gacgcgacat ggcattggta cggtacatca
    18541 ctgcacagtc gccgggttcg aattgcctgc tggcgacaag ttgggcgatg gtatccacca
    18601 caaaagatgc ttcgccgtaa tcgtcgcggg cctcgtagaa aaatatcttt tcgcccgggc
    18661 cgcggtcgct gaacagtctc ttcttgtggc ggttgcgcgc acggtcgatc acactggtgg
    18721 cggcatccag aatgttttga tgcgagcggt agttttgttc gagcaggacc acctgagcat
    18781 cagggaaatc ctgttcaaaa cgtttgatgt tgcgatagtc ggcaccgcgc caggcgtaca
    18841 cactctgatc ggggtcgcca acgcaaaaga tgtttttgtg atgagaagca aggtgtttca
    18901 ccaaggcata ctgcgcaagg ttggtatctt gaaattcatc caccaacaca tggcggaagc
    18961 gctgggcata cctatcacgc acggcaggga catcctccag caatcgtgcg gtgtacacca
    19021 acaagtcatc aaagtccacg gcattcattg agatcagccg tttttgatat tcaacataca
    19081 cacgtttcac cacttcgtca cggtaggtgt tgatcggata atcctctgca ccgatcaatt
    19141 cattcttcgc gcgtgaaatg gatgcgtgaa cgctggcggg acgatacaac ttttcgttga
    19201 gattcaactc acggatgatc gcttttacga tgcgttcctg atcgtctgaa tcgtagatca
    19261 caaaatttga ctcaagcggc agatggtccg cttcacgcct gagaatgcgc gcgcaaatcg
    19321 agtggaacgt gccgagcatc attccctcgg tggcttgctc gttcagcatg gcctttacac
    19381 gttgatccat ttccttcgca gctttattgg tgaacgtgac cgcaagaatc tgccatgggc
    19441 ggattccctc cgatgcgatc aaataagcga tgcgttgagt caacacccgc gtctttcccg
    19501 accccgggcc tgccacgacc aaaacaggcc catccgccgc tgtgacagcg ctcttttgtt
    19561 gtgcattaag ttttttgaga aattccatgt ccgccattat aaatgctctg gcgggggcat
    19621 tgcccccgcc agagcggtga atatcatttt gaatgttgaa gggaaatgct tacttcaatt
    19681 cagatgtgca gttggggcag cgggtagcct tgatggaaat ggtagagaag cagtgagggc
    19741 attctttggt ggttggggct gcagcgggtg ccgggggtgg ggtcttcttc atgctattgg
    19801 ctgctttgac aaccaggaag atgacaaagg caacgacaag gaagtcgatg accgattgaa
    19861 tgaacacacc ccatttgacc acggcagcgc caacggtcag ggatttctcg ctgaagttga
    19921 tcccgcccat gaccaatccg atcaatggca tcaaaatatc attgaccagc gaaccgacga
    19981 tcttgccaaa ggcaccgccg atgatgaccg ctacggcaag gtcgagcaca ttgccgcgca
    20041 tgacgaactc tttgaattct tttaacatgg gattctcctt gtgatgatgg cttggctttg
    20101 aattctattt taggaagggg ggcttgtcaa caaactgcac ccatgaaatg tctcgggtac
    20161 aaatccctgc ctgtgttttg gaacttctga tctctgattt gtatgacaag cggctttaaa
    20221 caagggttaa aaaaacagtt ctccagtagg gggtggattc cagtcttttg gtgtattttt
    20281 atacatgtac tggactggac aagccagtca taaagtttgt cgtgttgacg gcttaatgga
    20341 attaggtata attcggttcg cctgcagcca gaaaaatgca ggcagttatt atcccaattt
    20401 agaaaaggac ctagcggatg cctgatcttt tattagatgt aagagggctt gagactcaat
    20461 tcaagacccc agatgggatt gttcacgcag taaatggtgt ctctttcgga ctcaaagaag
    20521 gtgagacact cggtgtagtg ggggaaagtg gttgcggtaa gagcgttact atgcactcag
    20581 tactgggttt gattccctca cctccaggaa aagtcacggc gggggaagcg ttcttttttg
    20641 gccaagatct ccttaaaatg acacatgaag aaattcgcca tgtgcgcggt gcgcagatca
    20701 gcatggtctt tcaggatccc atgacctccc tgaaccctgt gttgacgatt ggccgtcaat
    20761 tgcaggaccc gttgatgttg cacaccggta tgaccaagaa tcaggcattg gaacgtgctg
    20821 ccgaattgtt gagtctggtt ggcatcccca atgccaaaga ccggctgaac gattacccac
    20881 atcaatactc aggcggtatg cgccagcgcg tgatgatcgc catggcgctt tcgtgcagcc
    20941 cgcaaatttt gatcgcagac gaaccaacca cggcgttgga tgtaaccatt caagctcaaa
    21001 tcgtggattt ggtcaaacgt ctgcgtgaag agttgggcat gtccattgtc tggatcaccc
    21061 atgatctcgg cgttgtggct ggtatcgctc aacgtgtttt ggtgatgtat ggcggcttta
    21121 ttattgaaga agcctccgtc gctgagttgt ttgcgaatcc atcccatcct tacaccctgg
    21181 gtctcttggg aagcctgcct cgtgtagatg ataccgaaca tggtcgtttg ttctccatcg
    21241 aaggacatcc cccagtcctc tacaaaaaac cgactgcatg tccgttcgcc ccgcgttgta
    21301 aatgggccat ggaaagatgt tggaaggaaa acccggccct tgagcagatc gcaaatgaac
    21361 accgtgtggc ctgctgggtc gacaccaaaa cggggagaaa ccgctaatga actcgcagaa
    21421 tacaaatgaa gtattgttga aggtcgatga ccttaagatg catttcccca tttatcgtgg
    21481 tgtgttccag cgccaagttg gtgctgttca tgctgtggat ggtgtttcct ttgatgtgat
    21541 gcgtggcgaa acattaggct tggtgggtga atcgggctgc ggaaagtcca ctacgggtag
    21601 aacggtttta cagttgtaca agccgaccgc tggcaatgtg gtgtttgatg gcgttaattt
    21661 ggttggcctg aaaaacgaag agatgcgcaa gacccgccgc aagatccaga tgatctttca
    21721 agatccctat gccagcttaa acccccgtat gacagttgcc gatatcatct ccgaaccttt
    21781 gatggttcat aatgtcggta ctggcaagga aattcaggaa cgcgtcgccc atctcctcga
    21841 actggtcaac ctgaatccgg cattcgccac ccgttacccg catgaattct ccggcggaca
    21901 gcgtcagcgt attggcatcg cccgcgcttt ggcgcttcaa ccttccttta tcgtctgcga
    21961 tgagcccatc tctgcgttgg atgtttcgat ccaggcgcag gtggttaacc tgctggaaga
    22021 attgcaggaa caattcaacc tgacatacct gttcattgcg catgaccttt caatggttcg
    22081 ccatatcagc aagcgcattg cagtaatgta tctgggtgtg atcatggaat tggcggagcg
    22141 cgacgaactg tatctcaagc cccttcatcc atacacacag gctttgcttt cggcagtgcc
    22201 cattccagac ccggttgcag attccaagcg caagcgtatc atccttgagg gagatgtccc
    22261 gtcaccggtg aatccacctt cgggttgtcg tttccgcaca cgttgcccaa ttgcagacaa
    22321 ggtttgcgct gaatcccgac ctgaatttcg tgaagttact ccaggccatt ttgtggcctg
    22381 tcatttggtc aaataatctt tcctggtaaa ggaggtgatt gctgcaagta agtgaccgta
    22441 aatgtccaca ctgtcaaaca atgtaagttt tttaaaccaa aatttccaaa ttttgcgagg
    22501 agaagaaaaa tgcgtaaaat gtttgcttta ctgagccttc tcgtcatcgc cagcatggcc
    22561 ctctcggcct gcggcggaac cgcccccacc gcggctcctt ccaccggcga tacgacgacc
    22621 gaagccaccg aggccccttc caccgatacc ggtagtgaag cccccgctgc tgaattcaag
    22681 tctgctgatc ccgccacact tgttgttgct gaatctgaag ttggtatcga cacccttgac
    22741 cccgctctgg cttacgatac cgcttccggt gaaatcatcc agaacaccta cgaaaccctc
    22801 gtgttctacg atggggaagc caccgataaa ttcgtaccga tgctggctga atcctgggaa
    22861 ctgtctgatg atggcatgac ctacaccttc aagatccgct ccggcgtgaa attccatgaa
    22921 ggtcaggata tgaccgcctc tgacgtggca tactccttcc agcgcggttt gctgcagggc
    22981 ggcacctcct ccccgcagtg gctgttggct gagccgttct tcggcgtcgg cgttgatgat
    23041 gtctccgtgg tcgtagaccc cgaaggtacc ctgtatgatg accgcgaact gctctccgcg
    23101 ttcgatgctg ctgctgtgaa ggctgcctgt gaaaaagttc aggctgcgat cgtggctgac
    23161 gatgccgctg gcaccgtaac catgaccctt gctcagcctt ggggtccttt cctctccacc
    23221 attgcccaga cctggggctc tgtcatgagc aagagctggg ttgttgagaa cggtggttgg
    23281 gatggttcct gcgatacctg gcagaacttc tacggcatgg cttcagctga cgatcccttc
    23341 agtggcatcg tcaacggcac cggccccttc acccttgaga aattcgccaa cggcgaagag
    23401 atcattcttg cgaaaaaccc caactattgg ggtgaacccg ccaagcttga tcgtgtcatc
    23461 ctcaagcagg tccctgaatg gggtacccgc ttcgcgatga tgcaggctgg tgatgcagat
    23521 ctcgctgttg tccccgccgc caaccgctcc cagatggacg aattggtcgg tatttatcag
    23581 gtgttcgacc tcgctgccaa tgcttatggc cctgaacagg aagtctgcgg ttatgatgac
    23641 accgctttgg ctgctgccaa gttcaccgct tgtgctgctg gcgaaaccgg caacggtgga
    23701 cctttccgcc tgcgcatcgg ccgcccggcg atcagcatgg acgtgttgtt gatgaactgg
    23761 aatattgcga cctccgaaga aagcccgaac ccctacatcg gctccggcgc tttggatggc
    23821 aatggtatcc ccgctgactt cttcagcgat gtccacatcc gcaaggcgtt tgcctactgc
    23881 ttcgattggg acaccctgat caacgacgtg ttcagcggcg aagctgtgca gtcctaccag
    23941 ctgcccctcg ctggcatgcc tggctacttc cccgatactc ctcactacac ctttgatccc
    24001 gcccagtgcg aagctgaatt caagctcgct gacgtggaca aggatggaat tgctgctggt
    24061 gacgatcccg aaggcgatgt ttggaccact ggtttccgtg ttcagatggc ctacaatcag
    24121 ggcaacaaca cccgccagac catcaccgaa atcctttccg gcaaccttgc ttccgtgaac
    24181 gaactgttcc tggttgagac cctcggtctg ccgtggcctg cttacctccg cgctcagcgc
    24241 gccaagcaga tcccgatcat gaccggtggt tggttggaag acattcacga tccgcacaac
    24301 tggtatcagc cgtacaccac cggtacctat ggtggccgcc agtccctgcc ggatgatgtc
    24361 aaggctcagt tccgtgacat tctcgaacgc ggtgtatccg agaccgatcc cgccaagcgc
    24421 gccgagatct acaaggaagc gaaccagctt tactacgatc tggctgttgg tattccgttg
    24481 gatgttacaa cctcccacgg tttctcacag cgctgggtga agggtgtcgt tcttaacccg
    24541 atcttccccg gtaactactt ctacaccatg tacaaagact agtcttcgga ctggttgatg
    24601 caaaaaattc ggaggcctgg caacaggcct ccgaaccaat agtgtcattc tgttttgcag
    24661 aataaaaaga atggaatatt ctttttattt cggaagccag aatatactga agatgaaaga
    24721 ggttgtttaa tgactacata catcattcga cgtttgttgt tggtgccggt actccttttt
    24781 ggggtgacga tattgatctt tgccatgttg cagttccttt ccccggtaga gcggacggca
    24841 ctttatgttc gcgatttccc aaagaatgaa aaagccatcg aaggtttgat caaacgctat
    24901 ggatttgatc agccgttcta tgtgcaatat tggacctggc tggtgggcaa agtagatagc
    24961 accacaggcg aacgtgaagg cggcatcctt tttggaaact ttggatattc ccgctccgct
    25021 tcacaaccgg ttgctgaatt aattcagcgt cgtttcccca acacacttga tttgaccatc
    25081 tgggcggttg ctccaatgat cttgataggt atttggctgg gggtgcaggc ggcagtgcat
    25141 cacaatggtt tcatcgatca ggctgcccgc gcatttagta tcgttggaac gtcctttcca
    25201 tctttcgtct tcggcttgtt gatgttgctg atcttttatg cgaatcttca ctggttctcc
    25261 ccgggtcgtc tttcagatga attcaaccgt gtggtcgaat cagaggcctt ccgtcagtac
    25321 accactgttc ttaccattga tgcccttcta aacggccgtt tcgatgtgtt ccttgatgca
    25381 ttgaggcaca tgttcctgcc catcctcact ctctcttata ttaactgggc caccttcctt
    25441 cgtgtaactc gcggttccat gctggaaaca ctgcgcatgg aatatgtcaa cacagcccgc
    25501 gccatgggtt tgcctgaaag agatgtgata ttcaaacatg cccaaccaaa cgccatgttg
    25561 ccggtgctga ctctggcggg gtttagtgtg gttggtttgc ttggcggtgt ggttatcaca
    25621 gaaacggttt ataacttccc tgggattggt tcggcagcgg ctcgtgcagc caccagtctg
    25681 gacgtggttg ccatgttggg ctttgccctt ttcaatggtt tcattctgat cgtggcaaac
    25741 ctggttgtgg atgtgctcta cgcgttcgtt gatccccgcg tacgcttatc ataaaggaga
    25801 attaaaaatg acagcagcaa ctcccagcgg cgataacacc ctgttaggcg agaatgtcgc
    25861 caccagaaaa attggacgcg ttgaacgctt tttaggcccc gaaaactacc gcattatcac
    25921 cggcctgctc aaaacacctg catccattgc tgggttcttt ttgatcggtt tctttatctt
    25981 cgttgccatt gcagcaccgg tcatcatgcc cccagtcact ccaaacgatc catacaaaat
    26041 tccgcgtgat ggcttccgtt cagatccccg ccccatgggc tcggcctgga cccgcaatgt
    26101 cccggatatt ccattttggt acacggcatt gaccggtaaa gataaatggg tccacattat
    26161 gggcacctct gaaggccaat acgatatctt ctacggcatg gtttggggta cccgcacagc
    26221 ctttcagacc ggtttggttg taaccttctc caccttcctc attggcatta tctacggttc
    26281 cgtttctgct tattatggcg gagttgtgga taatattatg atgcgcattg tggatgtgtt
    26341 gttgatcctg cccggtattt tggctgtgct cattcttgct gcggtgctca caccaaagat
    26401 cggtaagagt ttgataccag ccatggcggc gctcatcgcg tttgggtgga tgggatattc
    26461 ccgcatcatt cgcggcgata tcctgtctgt gaaggagcgc gattatgttc tggcggcccg
    26521 agtgatcggc gtcaaggata gccgcattct tttccgccac attcttccca acgccatctt
    26581 ccccaccctt gtgttggctt cactggccat tggtgatgtg gtgctctcct ttgcagccct
    26641 ttcattcctc ggcattggta cagaagttgg ctacgctgac tggggtcagg taatttcctt
    26701 cgcccgcaac tggatcacca gtttgaatac ctactggtat atcattgtct ggccaggggt
    26761 gaccctcgta ttgttcgtca tgggttggaa tctggttggc gatgccctgc gcgatgtgct
    26821 ggatccccgc atgcgcggca agacttaatc tgtgcagtac agattctttt ctcaattatt
    26881 gttggtcact attggaatgg ccctgctctt tgcagggcta ttcttcgata ttgacacagt
    26941 tgcttcccag gcagttgcca cacccaccat agatcggctt gcccaaccca ccctgcctgc
    27001 cgaccccaca caggccgatc aaggttcgca ggtgtattgg ctatcctgcc tgccttgtca
    27061 cggtgatcgc ggtcaggggt tgacggatga atttcgccaa acttatccgg tggaagatca
    27121 gaattgctgg atttcaggtt gtcatggcaa acgaccctac gataacggat ttacactgcc
    27181 gactgccatt cctgccattg taggtcccaa cactttgcaa aaatttgaga cggcggccag
    27241 tttaagatct tatatctttg cggccatgcc ttattggaaa cctgccagcc tgaccgaaga
    27301 agagacatgg caggtcacgg catacatact tcgtgaaagt aaacgcctgc ctgcggatat
    27361 ggaactcaac gcctccaacg ccgcctccgt gttggtcggc cctcctgttg caacttccac
    27421 ccctcaacct ggtcctgact ctgtaatcaa tctagatgag atacagagac cagttctaat
    27481 agttgctgtt cttgttgtag cgctgcttct tgctctgcgc attttcaaaa ccactgtcaa
    27541 taaagaataa tgaattaagc cgcccgtaaa ggcggcttaa tcgtataatt gaaacggact
    27601 tgatttgggc aggtacgatc ttttttgata cctgcttgtc tgtaagtaaa aaggaggtgg
    27661 ttgtttgcag atattggtat ttttgaatgt tttatgtccg ccaatgtagt tcgtaaaaca
    27721 aaatctcgtg aggagaacaa aaaatgcgta aaatgtttgc tttactgagc cttctcgtca
    27781 tcgccagcat ggccctctcg gcctgcggcg gaaccgcccc caccgcggct ccttccaccg
    27841 gcgatacgac gaccgaagcc accgaggccc cttccaccga taccggtagt gaagcccccg
    27901 ctgctgaatt caagtctgct gatcccgcca cacttgttgt tgctgaatct gaagttggta
    27961 tcgacaccct tgaccccgct ctggcttacg ataccgcttc cggtgaaatc atccagaaca
    28021 cctacgaaac cctcgtgttc tacgatgggg aagccaccga taaattcgta ccgatgctgg
    28081 ctgaatcctg ggaactgtct gatgatggca tgacctacac cttcaagatc cgctccggcg
    28141 tgaaattcca tgaaggtcag gatatgaccg cctctgacgt ggcatactcc ttccagcgcg
    28201 gtttgctgca gggcggcacc tcctccccgc agtggctgtt ggctgagccg ttcttcggcg
    28261 tcggcgttga tgatgtctcc gtggtcgtag accccgaagg taccctgtat gatgaccgcg
    28321 aactgctctc cgcgttcgat gctgctgctg tgaaggctgc ctgtgaaaaa gttcaggctg
    28381 cgatcgtggc tgacgatgcc gctggcaccg taaccatgac ccttgctcag ccttggggtc
    28441 ctttcctctc caccattgcc cagacctggg gctctgtcat gagcaagagc tgggttgttg
    28501 agaacggtgg ttgggatggt tcctgcgata cctggcagaa cttctacggc atggcttcag
    28561 ctgacgatcc cttcagtggc atcgtcaacg gcaccggccc cttcaccctt gagaaattcg
    28621 ccaacggcga agagatcatt cttgcgaaaa accccaacta ttggggtgaa cccgccaagc
    28681 ttgatcgtgt catcctcaag caggtccctg aatggggtac ccgcttcgcg atgatgcagg
    28741 ctggtgatgc agatctcgct gttgtccccg ccgccaaccg ctcccagatg gacgaattgg
    28801 tcggtattta tcaggtgttc gacctcgctg ccaatgctta tggccctgaa caggaagtct
    28861 gcggttatga tgacaccgct ttggctgctg ccaagttcac cgcttgtgct gctggcgaaa
    28921 ccggcaacgg tggacctttc cgcctgcgca tcggccgccc ggcgatcagc atggacgtgt
    28981 tgttgatgaa ctggaatatt gcgacctccg aagaaagccc gaacccctac atcggctccg
    29041 gcgctttgga tggcaatggt atccccgctg acttcttcag cgatgtccac atccgcaagg
    29101 cgtttgccta ctgcttcgat tgggacaccc tgatcaacga cgtgttcagc ggcgaagctg
    29161 tgcagtccta ccagctgccc ctcgctggca tgcctggcta cttccccgat actcctcact
    29221 acacctttga tcccgcccag tgcgaagctg aattcaagct cgctgacgtg gacaaggatg
    29281 gaattgctgc tggtgacgat cccgaaggcg atgtttggac cactggtttc cgtgttcaga
    29341 tggcctacaa tcagggcaac aacacccgcc agaccatcac cgaaatcctt tccggcaacc
    29401 ttgcttccgt gaacgaactg ttcctggttg agaccctcgg tctgccgtgg cctgcttacc
    29461 tccgcgctca gcgcgccaag cagatcccga tcatgaccgg tggttggttg gaagacattc
    29521 acgatccgca caactggtat cagccgtaca ccaccggtac ctatggtggc cgccagtccc
    29581 tgccggatga tgtcaaggct cagttccgtg acattctcga acgcggtgta tccgagaccg
    29641 atcccgccaa gcgcgccgag atctacaagg aagcgaacca gctttactac gatctggctg
    29701 ttggtattcc gttggaagtt gcgacctccc acggtttctc acagcgctgg gtacagggta
    29761 ttgtattcaa cccgatcttc cccggcttct attactacac catgtacaaa gactagtctt
    29821 ctgactggtc gatgtagact tactatgcaa aaaaagcaag ccgcccgaca aaggcggctt
    29881 gcttgtataa ttggcagatg cgttttttca aaaaggaatt tgccatgccc tcttccaaag
    29941 cgaatgtaaa actgttcctc ttttttctct ttctatttat catggcctgt ggtttttcct
    30001 ctgccacgcc ggaacctaca gccattcctg ccaacacaga aattcccctt cccacagcaa
    30061 ccccgatccc cagttcgttg acagtatgcc tgggtcagga acccaatacc ttgtatccct
    30121 atggcggacc gaacaccgcg gcccgcagtg ttttggccgc cgtttacgac gggccgattg
    30181 acatggtcgg atatgagtat cagccggtca tcctttcgca attgccgtct attgaaaacg
    30241 gcgacgccca gatcgtccca accacggttc agcctggagg tcaggcagtg gatgcggaag
    30301 ggaatctcgt cctgctggaa cgcggtaaaa aattccgccc tgctggctgc cgcggcgatg
    30361 actgcatcat cacctatgat ggctccaccc cgctcgaaat ggaccagatg gttgtcacct
    30421 tccgcttgcg cgaaggattg acctgggcag acggcactcc catggtttcc gatgattcgg
    30481 tgtatgccta cgatatttca cgcgattcca acgcggttgc aaatacctat ctcattgacc
    30541 gtacctatac ctatgaagcc actgacccgc tcaccgtgca atggtggggc atgccgggct
    30601 tcattgaccc aacctacttt actaatttct ggacacccgc tccacgccat ctgtggagtc
    30661 agtttccagc tgaccaatta cccgaaattg atatcgcctc acggacacca gtcggctggg
    30721 gaccatacgt cattcaagaa tggatcgcag gggaccatat caccctgaca aagaatcctt
    30781 attattcagc cgccgcagat ggttttccga aattcgatac cctgtcattc cgttttattg
    30841 ctgaccccga taccgcgctg agcgaaatgg tggctggccg ttgtgacatc cttgatccaa
    30901 gcatccgact cgataaccat gccagtctgt tgctggaaat ggaaaaaggc gaacaggcac
    30961 aggttttctt tacgcccggc atgaccattg aatggctggg cattggcaca accccatcca
    31021 cctacgatga tgggtacgac accacctacc aaaacgaccg ccccgacttt ttctccgacc
    31081 cgcacactcg tcagggcatt gcttattgtc tcgaccgcca atccgttgtc aacaatgttc
    31141 tgttcggcct caccaccatc ccagccacct atcttcccgt tgggcatccg ctgtatgaca
    31201 acaacctgca accgcttcca tttgatgttg ccacaggtgt ttcgttgctt gaacaggcag
    31261 gctggaagga taccgacggc gatcccacca ccccgcgtca ggcggtcaat gtaaagaacg
    31321 tgaaggcggg tacacccctt caactgaatt actacaccac cacagccacc caacgcagac
    31381 aggtggtgga aatcctgcaa aattcactgg cgcaatgcgg aatcggactc aatgtgcaat
    31441 acttctcgca aaacgatttg tatgcctccg gacccgatgg ggcactcttt ggccgccgtt
    31501 tcgaccttat tgaatatgcc atgggtgtag gcagcattga acctccttgc gattggttca
    31561 cctccgaaga aattccaacc agcgaaaact catgggtcgg caccaacgtt accggttacc
    31621 gcaatgaagc ctacgatact gcctgccgcg ccgcacaatt gtccctgcct gatgagcaaa
    31681 tctacgttga ttcgtaccgt caaactcagg tgttattctc ttctgaactt cccgccatac
    31741 cgttgtatta ccgcctgcgg gtggccgctg cccgccccgg cctttgtcac ttcgaactcg
    31801 acccgaccgc caacccgctt tggaatatcg aagcgatagc caccggcgaa gcctgccaga
    31861 attaatgcga cgactcttcc acattgggct ttttctaacc cttttgctcg tctcatgctc
    31921 cccgcagcct gcccgggtgg cagagccaac cagcattccc accccgacgc ttgcccctgt
    31981 tgtggcgcat gcgcctgaga ttcgatttgc attggttggc gaaccccaaa cggtcaatgt
    32041 ttggcaattg ttcgacaccc ccggcgcatc ctatgcagac tatgctttgc gcgccgactc
    32101 ttggccgcgc ctctatcacc tcctgcctcc agaatttact ttcgaacccc tggcagcatc
    32161 tggtttgccc accgcattca cgcaggaaaa cgatttttac gtcgctgccg tcaaactccg
    32221 taccgatttg aagtggaccg atgggtctgc attcactgca gaagatgtcg ccttcacggt
    32281 gaatgccgcc ctcgctttcg aactcggttt tgactggagc gaattctatc cgagtgatga
    32341 tctccaccat gccgaagcgc tggatgcatc cacggtgaaa tattatttca agcaggaacc
    32401 caatgtaggc gcatggcaat acggcgcatt gcagggccct gttgtgcaaa aagctttttg
    32461 ggaaggggcg gtaaatgccg cggccaattc cctgccaacc gaaaccctgc gaaccgaaat
    32521 cgcaaagaca caagccgacc ttgaaacggt caaacgggat gtggccgagt tgagcgaaca
    32581 ggtgcttgca ttgatcctga gcggcaaaca aaatcgaatt ttagacagtg acctcaaacg
    32641 aaagcagaac gaacagatct ttgtgcaaag caatctcgac cgcttgcttg aagaatatgc
    32701 ggcaaatatc gctgcggccc aatctgcttt gtatgcttct tctgatgaaa aagaacccct
    32761 gcttggcagt tggattcctc tcggcatgga agatggtcaa tgggtgaacg aagccaacct
    32821 tgagtttcca tttatccagc cgggttttga tcgtgccgtg tataaaattt tcgagagtga
    32881 gaccgaagcc ctgaccgctt ttgaaaatgg ggaagttgat gccatcctca cacccgctgg
    32941 aataagttcg aacatccccg gaatggtgta tagccccacg tatagtgcgc gtttccttgt
    33001 gttcaatccc ttgaaaacgc aatttgccga ccctgccttt cattctgccc tcagctgtat
    33061 gctggatcga aatacacttt cgtctgatag attgcaaaac caggcctttc cattcgatgc
    33121 ttttgtgctt tctccgcaat ggcatgatcc cgccgcacaa gatccctgca ccgggttgga
    33181 tgaagccaca cggatcgggc aggcagtgaa atttatgaag gatgccggtt atttttgggc
    33241 gaaagagcct gatgcaaacg gcgccgggca gggactgcgc tattcaaacg gtgatttatt
    33301 tccaacagcg gtcttactga gtcccacaaa agacgaagac ccattgcggt atgccgctgc
    33361 taattacgtg tcagaacgag ctgaatattt gggcgtttcc atcacagtac agacagcatc
    33421 catggatgat ttgatctatg ctgtgtacag ttctcaacaa tatgaccttg ccataacagg
    33481 ctggcggctg agtgaatacc cggcttattt gtgcgaaatg ttcgatgggc ggtcacagca
    33541 tttgtatacc agtgacaggt tccagtccgc gtgtgatgcg ctaaaaggag aatctgacct
    33601 ggagcgggcg cggcaggggt tccgtcagat cgagtctgct ttgatgatgg aactgccctt
    33661 cattccgctt tttacggtgg cgcgggctga tctgtatcaa aaaatcgcct atcctgccga
    33721 aaaagtcttg aatagttggg gcggattgta tggagcacct tcctttgcag ttccattgcc
    33781 ata
//
LOCUS       DAOQLC010000038        33293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_410_length_33293_cov_21.737860, whole genome shotgun sequence.
ACCESSION   DAOQLC010000038 DAOQLC010000000
VERSION     DAOQLC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 33293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_410_length_33293_cov_21.737860"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PL000_08710"
     CDS             <1..964
                     /locus_tag="PL000_08710"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC89021.1"
                     /translation="KAVWENSTKHVQTESIYNVEEAKAAIEMSAAIVGGRDKLRERPI
                     LSLMQCTAPPLGHDGGSLDAALLAAEVGIPTGFMTMAACATTGPTTMAGNLAVGNAEV
                     VAATALLQIAHPGAPVFYAAAQTASDLKTGAYTGGGPEDFLFGAATNVLADFYNIPLS
                     MGAFATGAKEPNWQAGLEGAMSSFMASVVMSDMLLGCGFLHGSRIWSFAEMMMDCEIF
                     SIVHKMMQGIEVNDETLAMESIANVGPGGHYLAQKHTRKHMRELFMPQFMDRRPYSEW
                     ETKKDDARDWALAKARKTLKEHQPDMLDERLSQELERIIKSVEK"
     gene            1056..2534
                     /locus_tag="PL000_08715"
     CDS             1056..2534
                     /locus_tag="PL000_08715"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC89022.1"
                     /translation="MQPKLTLLSDEIIARILEEAYELLRCPGVKVQNPEARDLLASAG
                     AIMYPDNNVVGIPEALVKKALESVPREFYLYDYDGNPTVHYGGDSVHFDPGSSGITML
                     NPETLEHDTTETEHLIRLLKVAEQIPQYDAQSTAVICHDVPKDIQDSYRLYLTLLYSK
                     KPIVTGAFTNKTVQEMIDMLALFAGSKEAAREKPRAIFDVCPAPPLIWSNFGAGNLIA
                     LARAGIPAEIVSVPLAGAAAPVTMLGTVTQHTAECFAGIVIHQLAHAGSPIVWGGAAA
                     IFDMRKGATPMGAVETAMLDCSYAQVAKTLNMPTHTYLGATDSKLVDAQAGLESGISA
                     MVGALSGINMISGSGMLDFLACHSAEKLVMDAEGIAMAKRMIGGVKQHTDTLATGFYD
                     DKINFKGGDFLKQKATMQLFQKEQHLPSSVIDRDSTRAWKAAGSLDAYGRAKLRVNEL
                     LASYRRPEIDPVKESRLHAFILDLAQKCGLEELPKLEDFQPA"
     gene            2664..4142
                     /locus_tag="PL000_08720"
     CDS             2664..4142
                     /locus_tag="PL000_08720"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC89023.1"
                     /translation="MQPKLSLLDDQLIERIIEEAYQLMLKPGIKVQNPEARQLLADAG
                     AQVDEETMVVRIPEQIVRTALQTVPRQFFLYDYDGNPAVQYGGDAVQFDPGSSGISIL
                     IPETLEHKTAETADLIRVIKVAESLPQYDAQSTAIVCHDVPKDIHDLYRLYLVMMYSK
                     KPIVTGAFTNKTVHQMIEMLAIFAGSHENLRKYPRAVFDACPSPPLIWSNFGAGNLIA
                     LARAGVPAEIVSMPLAGAAAPVTLLGAVTQHAAECLSGITIHQLACAGSPIVWGGAPS
                     TFDMRKGGTPFGSVETAMIDSSYAQVGKTLGLPTHAYLGATESKILDMQAGLESGMGA
                     MIGALSGINMISGSGMVDSLLCQSPEKLVIDAEGIAMAKRMVQGMKIQTETLATGFYE
                     GVNFKGGDFLKQKITMKLFKEEQHMPSKVIDRDSMRGWKESGSQDTFSRARTRVDEIL
                     ASYRRPELDPAKVAELHAYVLNLAQQAGLEQLPVVEAMEVAG"
     gene            4195..5979
                     /locus_tag="PL000_08725"
     CDS             4195..5979
                     /locus_tag="PL000_08725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNC89024.1"
                     /translation="MLNLAQLLRVPHVDNVLPYDVSPDGQRLIFSWNKSGKWELWEAG
                     ESGTRELMLGLDGAKFSPRFSPDGSMLAFALDLDGSESYHIAIHDFTTNTTTDLTPSI
                     AYAHQPNVSWSPDGKMLAVLSDMKGQFAAYLLPIDGSPVRLLKNIFHPCWDAVWSPDG
                     QWIAIESESTASDRSIHVVPVGRTRKGPKVNTVQLKVNGRVLNAQHPAWSPDSKFLAF
                     CGENVEWHDIGLFNVETQEITWVNESVGDDTQPAWSRSGDTLRVGWVHSEGAQTSFQF
                     KKGSADVAQIQVGAGVHSFPKFTAEGVVILYEDSSRPCDVWMLNLEDGTCKQLTDSLP
                     AELKDVQFVQPEEVWYAGMDGVQVPALLYRGQSERAVIDIHGGPNWHVQSLWSPATSY
                     LVSRGWTVLMPNYRGSTGYGKKWQNASRYDMGGVDTDDCAAGVKYLVENGLAEKNKIT
                     VTGRSHGGFLTMSCLTRYPDLFAGGSAVVPFLNWFKSHKDSREDLQHWNIENMGDPED
                     NKELWHSHSPYFFLDKINAPVQMICGGNDPRCPASDSIAARDKLVELGKEVELLLYKD
                     EGHAFLNIENVIDAEEKSAAFLEMVLKK"
     gene            5999..7417
                     /locus_tag="PL000_08730"
     CDS             5999..7417
                     /locus_tag="PL000_08730"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC89025.1"
                     /translation="MEPLKFLSENDVKAMHEATLRIMSEVGLIWTHKPSLDILLGAGC
                     TIKGNRVCMSPELVMDCIAKANKRPKIRGRNGVTNELGGGNLYFHNLGGARDVFDART
                     GTRKIATDQDVIDATRVLDALPNCHTVTPFFTPQDVPGELMSLYMYRHSLPYTTKPLQ
                     GPGIQFAEEVKYAVEMAQVVGTPPEELTLSLSPVSPLTIHDPAAAAIVEMAKAGVICS
                     NLPAPTGGATSPMTITGSLVQQSAETLALVVLAQIVNPGCGVVYCGRLGMLEPRTGLI
                     WGGVELGLSSAGTVQLGHYYGFSVNVYGFSTNAHTLDAQNAFERGLNAAIPALAGADE
                     LSGIGEMEAGVMGSYAQMVLDNELVGSVMRLRSGLSADVEHLAVEIIGNVMDGTRNYL
                     GQKHTLKHLRAGELALTKLAERNSWETWEDKLERKQMADYAMEESERILREHQVPPLE
                     PQQEKELDRIMAAAEKEMVKKK"
     gene            7452..8609
                     /locus_tag="PL000_08735"
     CDS             7452..8609
                     /locus_tag="PL000_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNC89026.1"
                     /translation="MLKQANAISEELIEWRRDFHMHPETGFDVHRTAGIVADELEKMG
                     YRVQRGVGKTGVVADIGEGGKLVAIRADMDALPIFEQNETDYVSQNTGKMHACGHDSH
                     TAMALGAAQILAKEKFTGRVRFLFQPSEETADEEGKSGAQRMYEEGATKGVDYVIAQH
                     VDPTSPVGTIAIEAGPSGGGVDTWFATINGKGGHGAYPHTTVDPFVLLAHVIMGLNNI
                     SSRRMNPFEPVALSIGSIIGGDAENVIPDQIKLKGTLRFTSLETQKLLRDEMRRVFEI
                     TKTLGGDYELDFLFGGLPMINDGHVSKVIDQVGKDLLGAENVHPMHKSLGAEDFPEFL
                     RDAPGAMYTLGTMIAGREVYELHHPKFDLDERALPIGTAVLVETTLRFLKE"
     gene            8629..9501
                     /locus_tag="PL000_08740"
     CDS             8629..9501
                     /locus_tag="PL000_08740"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC89027.1"
                     /translation="MHTILKSEKKEVVIGIDKPFVMIGEKINPTGIKKLGQALVDKNM
                     DYVKQLAKRQVEWGADVLDVNVGHPQIDEAAIIPMVVEAVKSVVDVPLCIDSNDPKIL
                     EAGLKVAPGKPLVNSVNGEEKQLETVLPIVKDRGAAVIGLTIGDDGIPPTAELRLAAA
                     AKIIERAAKIGIPIEDIIIDPLVMTVGHDSKAATVTLKTIELLVKEFGVNINLGASNV
                     SFGLPDRHSVNAAFLALAMQTGVTCSITDPIKLGSTIKATDLLLGRDANSMRYLKYFR
                     ATEKLREAEAAAKQ"
     gene            9659..10951
                     /locus_tag="PL000_08745"
     CDS             9659..10951
                     /locus_tag="PL000_08745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC89028.1"
                     /translation="MSFLAVLAVLLLTYQYTKREWEYSQKEMISQAGLLLDTISLTLR
                     DPLYNLEIDELKDLAGKIGENPQITMFVVYDKTGRILVDSSKPELEFSQEVDVLGYNL
                     VTTPTHELYISWQGTQLVSGKSIRIGNQTIGAIAIGFSTEKHDQKVKDLTLQGLTLAL
                     VVIFLGGLFTFGTARQITSPLSELARVANKMKAGDLTVRANQPRVNDEIGQLSTAFNQ
                     MAESIQQRQSELRELANGLEKTVNERTAELQEQAKILEEMAITDPLTQAYNRRQFYKL
                     AKIEMERAQQQDGKPLSVIVIDADHYKRVNDTFGHQIGDLVLIKLVEICHNSIRKTDV
                     FARYGGEEFVILMPETDSETARVIAERIRERVESTKLPVDGQLVQITISLGVSTYSNA
                     YSLSFDSLLTQADRALYKSKRLGRNRVTVWADEMLVPG"
     gene            10994..12067
                     /locus_tag="PL000_08750"
     CDS             10994..12067
                     /locus_tag="PL000_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC89029.1"
                     /translation="MSKKLYLYSLVLLLIACSTQSPAISPPTPVATATQTIPPSQTAS
                     PTFASTFTPIPTATPIPYPMSIIAMRNGEYPGSEITIVKELDRGSNYRRYYSYYLSEG
                     LKIYALLTIPNGEPPEGGWPAIVFNHGYIPPDVYRTTERYIAYVDEIAKAGYIVFRID
                     YRGHDASEGEPTGAYGDPGYQIDVLNAVAAIKQLPEVNPDKIGMWGHSMGGYLTLRAM
                     VISQDVKVGVIWAGVVASYQDLLYNWRRTGSFTPSPSSRGVGWRTRWIEEFGTPEQNP
                     VFWDSISATSYLSDLSGPLELHHGTADEDVPLDFSIRLAEMARNAGQTADLYIYEGDN
                     HNISANFSIAMERTIKFFDSYLK"
     gene            12118..12885
                     /gene="fabL"
                     /locus_tag="PL000_08755"
     CDS             12118..12885
                     /gene="fabL"
                     /locus_tag="PL000_08755"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HNC89030.1"
                     /translation="MFQNKIALVTGSGRGIGRAIALHFAKHGADIVVNFFRNRTPAEE
                     TAREVEKLGRRALLIKADVGDLDDLNRMFDEIQAGFGGLDILIHNAASGYNRPALEQK
                     PKGWDWTMNINARALLFAAQRAAALMEIRGGGKIVSISSAGATRVLPDYVVVGASKAA
                     LESLTRYLGVELISKGINVNAVSPGVVETDALHHFASMGNQDNILQNFIAQVPAGRLI
                     TPEEVAEVTAFLCTPAAAMIVGQTIVIDGGYTLPIPQ"
     gene            complement(12889..13737)
                     /locus_tag="PL000_08760"
     CDS             complement(12889..13737)
                     /locus_tag="PL000_08760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC89031.1"
                     /translation="MQKIFALLEKRSPYFWIFTSLVIVGLLGVVDYLTGNEFSFSLFY
                     LIPVSLTAWYVSSAAGAIFSILGAVTWLIADLVIGENYSSKFVYLWNTLIRFGFFAIV
                     SYLMSELHRAQNKMQTLARTDFVTGAFNSRYFHELLKTELDRSSRYKRPFTLVYLDLD
                     NFKQVNDRFGHDEGDKLLTCIAQELKQAVRKSDIVARLGGDEFAVLFPEAGRQEAEAI
                     IAKVHHHISEQLKQTHSYVTFSAGAVTFEASPISPAETIRIADKLMYAVKNSTKNGVR
                     FSTYDG"
     gene            complement(13861..15534)
                     /locus_tag="PL000_08765"
     CDS             complement(13861..15534)
                     /locus_tag="PL000_08765"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNC89032.1"
                     /translation="MRKIPVYLLCLAMLAACVPATNAPAVATNPIPTATASPQPVSTN
                     TPMPAALWIAPAVPAVLHEAAISSGLPITEDPALATQKLDMSDSGSLWIYALVAPFPT
                     LTDDVALQDLLSTWKGASSGPLNGHSVLLAESTLRAFASVWGEPASGSVRSVPAGEIL
                     DTAWKESAWAIIPFEEIQPKWKVLSVDGQSPLRKSFDAEVYPLKIHFSLSQPEEFALP
                     ETNRDPAKLTTVILTGVTALVRATAVTMELRGVTFPGEFVRDELREADITHISNEVTF
                     DKDCPTPSSGYRNFILCSDPKYMDLLLDVGVDVVEVTGDHLRDRGEVALLDTLAMYKA
                     NNLPYYGGGINAEEARQPVLMEVNGNKIAFMGCNGKREGKYPKASATSPGPALCDFDF
                     FGDQMQALKAQGYNIIFTFQHEECYSPGPCYTHEEGFRRAADEGAVIVSGSQAHFPHV
                     MEFHGDAFIHYGLGNMFFDQMTYNLPSGEVIDETRREFHDRHVFYDGRYLGVELLTSI
                     LEDYARPRPMTAAERLQFLSDYFYYSGWMPLLPTPVPQPTVTLTPIALP"
     gene            complement(15616..17427)
                     /locus_tag="PL000_08770"
     CDS             complement(15616..17427)
                     /locus_tag="PL000_08770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNC89033.1"
                     /translation="MPRIIFLERLESYLAGRFTLIKSSFFVRQPNWNGHRATRLGFLL
                     ISILSLVACQPSLWGAPPPPPTFAAATPSAMPRIPTLAPTITPMPPTAVPTPVVLNAA
                     QVWASPATPLALREKLQHWGFQVLTTNQASANLQIDVAQPITGAFDTSTWTYALVAPF
                     PTIIDGLSSQELISVWSGASFGPFAGIPILMEESTMMTFTALWGAPAAGSVQVVPADQ
                     LLDTAWNQMPSWAIIPFEKIEPKWKVLTIDGQSPIQKHFDPVIYPLTVTYRLTCAEPC
                     EVPAELDFSYQNRDPNKLATVIMTGVTAMVRATAKTMDVKGITYPGEEIRDLLREADV
                     THINNEVPFFTGCPSPDPNQEKQIFCSSPRYMELLTDIGTDVIELSGDHFADYEDVAM
                     YETLDIYKQYNLPYYGGGYNVEDGRKPLLMEVNGNKIMFIGCNYKTIYASATDTVPGA
                     VPCDFDYMTEQIAYYRSQGYLPISTFQYHEFDTAKARPQQIIDFRRMADAGAIIVSGS
                     QAHVPQEMEFYNGGFIHYGLGNLFFDQIAKRGPRLTEREFIDRHIFYDGRYLGVELIT
                     AWLTDYARPRYMTELERTRFLTDIFKESEWGFLQVGQ"
     gene            complement(17496..18611)
                     /gene="menC"
                     /locus_tag="PL000_08775"
     CDS             complement(17496..18611)
                     /gene="menC"
                     /locus_tag="PL000_08775"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HNC89034.1"
                     /translation="MKIDSAVLHHLSMPLVAPFETSFGRETDRECVLIELRSEGLVGY
                     GECVASRDPGYNYETTGTAIHILKDFILPLILGVDVKDALDFQTRVAGIRGHHLAKAG
                     VEMAIWDLLGKRSGKSLREMLGGAREKVEVGVSIGIQESAQGLVKTAAEFIEQGYARV
                     KIKIKPGRDVEDAAAVRKAFPDLRLQVDANSAYSMDDAPLLKPLDKMNLLLIEQPLFE
                     DDIWDHHKFQEQFETPVCLDESIVSPRHARYAIEMKACRVINIKAGRLGGISQGVMVH
                     DMCMEKSLPVWCGGMLETGIGRASNLALASLPNFVLPGDVSASDRYYKRDITNERFVL
                     NPDSTITVPNGPGLGMTIDEEALKLFSLATLSIVANS"
     gene            complement(18802..19332)
                     /locus_tag="PL000_08780"
     CDS             complement(18802..19332)
                     /locus_tag="PL000_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC89035.1"
                     /translation="MTFDPVFLGSLTLVLTAFGGAFLAALWISLVVWTYRDIRARARD
                     PLTQTLAALLVAVLNLPGVLVYLILRPPRTLEEEYQRTLEEEALLQALEDLPLCPGCE
                     RRVKEDWQVCPNCHTKLKKSCHNCGKFMELPWNICPFCGTPAPGMRLESTGMDDALRN
                     LKLTDDSTEVKVDSAG"
     gene            complement(19400..20668)
                     /locus_tag="PL000_08785"
     CDS             complement(19400..20668)
                     /locus_tag="PL000_08785"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HNC89036.1"
                     /translation="MILQRRILALLAFFLATSCASTPDPLPASVPLAAPTLTPFQPLP
                     EATSTPDVAAIPAEPTYTPLPRQNTAIEILPTPLLMDPSANGSAGDFSLMNINNPLTG
                     LPVNDPALLERRPMVIKVANAPDTIRPQSGLTLADVVYEYYIEWGDTRFIAVMYGNDS
                     PKVGPVRSGRYFDEHVARMYNAFLVFKFADPREYSYLKNSTLNEFLVVPGGSGCPPFI
                     FGPDKKRDDYNNFFFVTAEWAACAASKGLDNSPQVIRGGFFSDAPMEGTTTASRIFSY
                     YSRQSYNYWEYDVITHKYFRYQEANDMLNGKPEAYAPLSDAETGLPVNAENIVVIFVP
                     HTFANEFNAKDEVYNIDLIDYGNAFVFRDGFVVPAKWERTDINQPLLLTNLDGSPIYL
                     RPGRTFYEVMGFTSTYTQDGSDWHFKFETP"
     gene            20739..22292
                     /locus_tag="PL000_08790"
     CDS             20739..22292
                     /locus_tag="PL000_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89037.1"
                     /translation="MLILISCLLLFLTALILLVLGVTQPNARYTWLVAIGGAMLSLAS
                     VFIWLARIPVDFTLPAWQPLTLFQNPILFRADAISWPYAVAISGITLTILLTEVTRPN
                     STSLYAWAGVLALGGLGILAVTANNPFTLLLIWSALDLAELITQLRSVDGPSNNERVV
                     ISFSTRAFGTFLLLWANVISIAQGSTFDFHSIAADSGLYLVAAAGLRLGVFPLHLPYS
                     SESVLRRGFGTALRLVAAASSLILLAHVPAGSLNSSVTPFLLLFALIAAVYGGWMWLR
                     APDELTGRPYWIIGIAALSITATLNGNPLGAIAWGCALILVGGALFLSSVQHKWLSRF
                     LLVGAWSLSSLPFSLTASVWLGNLNFFVPFGVAAQALLTAGLIRHSLRPGDRGSLDSQ
                     ENWVNRIYPAGIILPAVLQIILGLIGWDGARQIGAWLLAVIASLLILGLVWATPRFRI
                     LNPIRAHWVNPIAANTPSVFYSSFWAVYRVFERINLTITTALEGNGGVMWTLLFLVLF
                     ISLLTQGLP"
     gene            22294..22905
                     /locus_tag="PL000_08795"
     CDS             22294..22905
                     /locus_tag="PL000_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89038.1"
                     /translation="MATDIISWIAVGMILATSAIILISRDWRIPLGALALQYLAAFWL
                     VTRHLPFVMGSAKLITGWMVVAAIGMTRLGLTATEEKAEDTSLQSGRWFRGILIGIVA
                     LVTAGATPRIETSIPGLGLPVIAGSILLIGAGVIHLGVTSDILRVTLGLLTMLTGFEV
                     LYAAVESSILVAGLLALTNLGLGIAGSYLLIAGSIPLEVEDEE"
     gene            22902..24299
                     /locus_tag="PL000_08800"
     CDS             22902..24299
                     /locus_tag="PL000_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNC89039.1"
                     /translation="MSAPIIWILIPFVAGVLILFLLQERISSFVGGVVSMLLALTALI
                     FPIDTALLIGSVSIKISSTVQFFGRSFALNTADGPLLAIIYGLATLWFFGTQASGTAN
                     RFVSLGLMIVALLTASIAVEPFLFAALLIDMAAMLVIPLLVPLHQRPGQGIVRFLIQQ
                     TLAMPFVLFSGWMLAGVEASPGDIGNTIQAGTMLSLGFAFLLAIFPLYSWIPMLTEEA
                     SPYATGFLLWALPTFTIIFLLNFLDRYTWLRSTPQLSNAIQFAGVLMVASGGLFASQQ
                     KHIGRMMGYASIVETGLLVLAAGLKSTEVVNVTFLLLTPRGLELAVWALALSIIKRKV
                     YSLRFSEVRGMARQYPIAVAAIVLAHLSMAGFPLLAGFPSRAALWQELTPESLTMSFW
                     VLIGLLGLLMGAMRTLAVFVMAEENRTWEINESWVQMGMLGVGVIGLFILGIFPQTLQ
                     PFIASLPALFEHLGQ"
     gene            24361..25548
                     /locus_tag="PL000_08805"
     CDS             24361..25548
                     /locus_tag="PL000_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89040.1"
                     /translation="MEFEQIIKRLEWLDKQQRKNQDALTSLDGRLESFETTINAVSKQ
                     MKGFTKQITDVSPMEKRLDQFETLMTRQRAEFVKLLEENEKAQTRTTKENLKYVQTEL
                     TEIQKVIANIKTTTNLTEIKKQLKERADEMQRVLNNVADLKFRVDEATRTKDDAVHAL
                     KAIEETRKNDLKRVADIQGEVTALRKRVDENRDKATITADSIRNVENRFTDLIASELE
                     RKQAQAAFLEQQTLAQIDRDRAWKEWKEKYESFQKEAANLDIHIQNLDEALRGAKKAQ
                     ETYLELNTKLERRINEVTEMQRLAEDRLRQEWVSFKTDDQKRWTGYSLSSEESFRDIR
                     KEVQKLETRITPFDDITQVLQDQMHQTTDATEKQLQEMMNVLHEWMLSYQRIMGHGKK
                     SKK"
     gene            25614..27509
                     /gene="selB"
                     /locus_tag="PL000_08810"
     CDS             25614..27509
                     /gene="selB"
                     /locus_tag="PL000_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNC89041.1"
                     /translation="MRVIGTAGHVDHGKSTLIAALTGTHPDRLKEEQAREMTIELGFG
                     WMNLPNGEEVGIVDVPGHRDFIENMLSGIGGIDAALLVIAADEGVMPQTKEHLAILDL
                     LQIPAGLIVLTKTDLVSDSTWLDLVETDIRGAVSGTVLQDAPILHVSAKNKLGLKELT
                     ASLQTLLENKPARPDLNRPRLPIDRVFTMSGFGTVVTGTLSDGHLTVGDEVEILPSGQ
                     KGRVRGLQTHKKKEEQALPGSRTAVNISGVNTEQIQRGEVVSHPNQYTPTRRIDARLR
                     LLKDISKSIKHNDEVKFFTGASETIATLRLLGAEELRESEEGWIQLELRDPIVAVRGD
                     RYILRRPSPSETLGGGVIVDHQPKGRHKRFDETVLKSLESLAQGSPADILFEAALALN
                     TASVKEMVARSRLESAPAGEALQELLADGRLVLLEEGEATAASDLLAIAIPHWSILQE
                     KTLQLVKAYHQQFPLRRGIPREELKSRLKLTPRVFNATVNFLTRHSALTDLRGSVAQT
                     THEIKFNGQDQARVNGLMRKFEANPFGPPSVKECQSEVGEEAFNALLEMNELVSVSPD
                     VVFKKQDHELIVIKIRAELKNKGTITLAEVRDLLGTTRKYAQAILEYMDAINITIRDG
                     DARKLRN"
     gene            27532..30669
                     /locus_tag="PL000_08815"
     CDS             27532..30669
                     /locus_tag="PL000_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89042.1"
                     /translation="MGNSFKPPTFDSEEKSHQAFLLRAIVWGMILVPIPYLIFIAITN
                     PSGFNRVLAQSAFGEAINLLLLFMLRKGIVRLAAILQIVMLWIFFSITAVSGEGVQSE
                     AYLMGFPLVILIAGVLFGGRAASYVTVASLLTGLVLIYAETQQAILSTNLTRSMNMWV
                     ISLVIFPIITTLQYLSSLSVQNALKRARISERKYMLLSNISTDYVFETKVDEAGNNTL
                     FWVGGAFEKMTGYTLEEYLAAGSWSAHVHPDDIEKDIRDMEALARNEDIKSEIRTINK
                     NGETHWERIYAHPIWDDYNNRLAGIIGAVQDITESKHAEETLIATLSQTAEMVRKIPD
                     MAWLKDVNSKYVAVNEKFASVAGRPMEEIIGKSDDEIWNPEFAKNYREDDLLVIETLK
                     LRRVEEKQMDRLGREYWVETIKTPIFNGKGEVIGTTGVARDINDRKEAELAEKRRREM
                     LEKVIELGKLVTEASDIKGTIKKIWHGVHDDLQFDRLAIFLYNAERNQMDDTLGTDIN
                     GQMTDNYGLSFPVGEDTDGSTFAYLLEKPDGFYFTKDYAKENNIPPGHEMYHVKEYTA
                     VAVWAGNKPVAVICADNLITLRPITNEQLEGLRLFAGYAGLAIENSRLHSAIQNELDL
                     QKSAEQREARRRATLEKVIQLGQSMTEVHDLRATLERIWHGVHDELAFDRLGIYMYNP
                     VRKSMDGTFGTNDRGEMVDEWETWKSIDQGNEEANSFLYLLNNPDSIYLTRDYESEHK
                     SAINDIMLGVKDFAAVAAWVGEKPVAVICVDHKISGRPISDEQLEALRLFAGYAGLAI
                     ENARLNTALQNELTQRKTFIEELEAKNAELERFTYTVSHDLKSPLVTIKGFLGYLEQD
                     ALSGNFDKFKQDVSRIETAVGKMQNLLRDLLELSRIGRLINTPADIQFDEIVKDALEI
                     VHGQIEGKSIHIIHKQTGIMIHCDRVRLTEVLQNLIENAIKFMDNQPSPRIEIGAFEN
                     ERGQAIFYVKDNGAGIAPEFHEKIFGLFNKLSTESSGTGIGLALVKRIIEVHGGRIWV
                     DSQINRGATFYFTLS"
     gene            30685..31356
                     /locus_tag="PL000_08820"
     CDS             30685..31356
                     /locus_tag="PL000_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNC89043.1"
                     /translation="MCGRFTLTVDPAELQDTFSEFTFPTQFSPRFNIAPTQPVLAIPN
                     DAKNKADFFLWGLIPSWAKDPSIANKLINARGETIAEKPSFRGSFKYKRCLILTDGFY
                     EWKSQPGAKTKTPYFIHMKDRKPFAFAGLWDEWHSPDGGSVRTCTIITTEPNELMSTL
                     HNRMPVILDSQNYADWLDAAPRTPDSLLHLIRSFPADKMSAYPVSTMVNAPGNDRPEL
                     VVPVQ"
     gene            31439..32641
                     /locus_tag="PL000_08825"
     CDS             31439..32641
                     /locus_tag="PL000_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC89044.1"
                     /translation="MQNDFNEDSTSSAKPKEAKQAEPVRQRSFFTRWMNTLVQMGLGE
                     SLLRSGTNLFSIAAIVVVILLVQAFNTQRANPASGNSDQASGPAPTVEVDIHSIPALE
                     NPLITGISRSAQIHTNIPSRPRNEITNYTVQEGDTVFGIAEKFGLQPQTILWGNYNIL
                     LDDPHSLKPGQELNILPVDGVYWEWLGGIPFGDWAEFYGVTAADIIEYPSNKIDPNAV
                     GDYANANIKTGTWLIIPNGKREFVNWSAPLGVTRENPASARVLGQGACEPVSGGAVGY
                     GTYVYPTNKHFLSGFDYNEKTNHRGIDLAGSEGEGVYAADAGVVVYSGWNNYGYGNMI
                     MIDHGNGFQSLYAHLSAIYRGCGQSVGQGEAIGAVGSTGRSSGPHLHFELMTATWKVN
                     PWDYLPPP"
     gene            32687..32760
                     /locus_tag="PL000_08830"
     tRNA            32687..32760
                     /locus_tag="PL000_08830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32721..32723,aa:Val,seq:cac)
     gene            32908..>33293
                     /locus_tag="PL000_08835"
     CDS             32908..>33293
                     /locus_tag="PL000_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019112401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNC89045.1"
                     /translation="MKVAILAGGLGTRLSEETTVKPKPMVEVGGKPILWHIMNIYAAY
                     GFKEFVVALGYKGEIIKDYFLNYHYRSRSITVQLKTGRVTAHNIEGEDWTVHLLDTGA
                     DTNTGGRVKRVAEFIGNETFMLTYGDG"
BASE COUNT         8537 a         8537 c         8514 g         7705 t
ORIGIN      
        1 gaaagccgtt tgggaaaact ccaccaaaca cgtgcagacc gaatccattt acaacgtgga
       61 ggaggccaaa gccgcaattg agatgtctgc ggcgatcgtt ggcggcagag acaaactccg
      121 cgaacggcca attctctcgc tcatgcaatg cactgctccc ccgcttggac atgacggcgg
      181 atcgctcgat gcggcactcc tcgctgctga agttggaatt ccaacaggct ttatgaccat
      241 ggcggcatgt gccacaacag gccccaccac catggctggc aatcttgcag tcggcaatgc
      301 agaagtggtt gccgccaccg cgcttttgca aattgcccat cccggtgccc ccgtttttta
      361 tgccgctgcg caaaccgctt ctgatctaaa gactggcgca tacacaggcg gcggacctga
      421 agatttcctt ttcggtgcag cgaccaacgt cctcgcagat ttttacaaca ttccgctttc
      481 aatgggtgcg tttgccactg gcgcaaaaga accgaactgg caggcaggct tggaaggcgc
      541 catgtccagt ttcatggcaa gcgtggtcat gtccgacatg ttgcttgggt gcggattcct
      601 gcacggcagc cgcatctggt cttttgccga gatgatgatg gactgcgaga tcttctccat
      661 cgtccacaag atgatgcagg gaatcgaagt caatgacgag accctggcga tggaatcgat
      721 tgccaatgtc ggccccggcg gacattacct cgcgcaaaag cacacccgca aacacatgcg
      781 tgaattgttc atgccccagt tcatggaccg ccgtccctac tccgaatggg aaacgaaaaa
      841 agacgatgcg cgtgactggg cactggcaaa agcccgcaag accttgaagg aacaccagcc
      901 tgacatgctg gacgaaagat tgagtcagga actggaaaga atcatcaaat cggtagagaa
      961 ataaagagtt aacaagttgg aaggttcaca agttcacagg ttgacaactt gtaaccttcc
     1021 aactttccaa cattccaact cattagaggt gtctcatgca gccaaaactc accctgctca
     1081 gcgatgaaat catcgcccgt attttggaag aggcctacga attattgcga tgtccgggcg
     1141 tcaaggtaca aaaccctgaa gcacgggatt tgctcgcatc ggcaggcgcg atcatgtatc
     1201 ccgataataa tgtcgtcggg attcccgagg cgcttgtcaa gaaggcactc gaaagcgtac
     1261 cgcgcgaatt ctatctgtac gattacgatg ggaatcccac cgtgcattac ggcggtgact
     1321 cggttcactt tgacccgggt tcgtcaggca ttaccatgct caaccctgaa acacttgagc
     1381 atgacactac ggagacagaa cacctgatcc gcctgttgaa ggtcgcagaa cagatcccac
     1441 aatacgacgc acaatccact gcggtcattt gccacgacgt tcccaaagac attcaagatt
     1501 cataccgtct gtacctcacg ctgttgtatt caaagaagcc catcgtcaca ggcgcattca
     1561 ccaacaagac cgtacaggaa atgatcgaca tgcttgcact gtttgcgggc agcaaggaag
     1621 cggcccgtga aaaaccgcgc gccatcttcg atgtctgtcc cgcgccgcca ttgatctggt
     1681 cgaacttcgg cgcagggaat ctgatcgcgc ttgcacgcgc aggcatccca gccgagatcg
     1741 tctctgtccc gttggcagga gcagccgccc ctgtgaccat gcttggcacc gtcacccagc
     1801 acacggcgga atgctttgcg gggatcgtca tccaccaact tgcacacgca ggctcgccca
     1861 tcgtttgggg cggcgcggcg gcgatcttcg acatgcgcaa aggcgcaaca ccgatgggcg
     1921 cggtcgaaac tgccatgctt gattgttcat acgcgcaagt cgcaaagaca ctcaacatgc
     1981 ccacgcacac atatctcggc gcaaccgatt cgaaactggt tgacgcacag gccgggcttg
     2041 aaagtggcat ctccgccatg gtcggcgcgt taagcggaat caacatgatc tctggctcag
     2101 gcatgttgga cttccttgcc tgccacagcg ccgaaaaact cgtcatggat gcggaaggca
     2161 tcgccatggc aaagcgcatg atcggcggcg tcaaacaaca caccgacacg cttgcaacag
     2221 gtttctacga cgacaagatc aacttcaaag gcggcgattt cctcaaacaa aaagccacca
     2281 tgcaactctt tcaaaaggaa cagcacctgc ccagcagcgt gatcgaccgc gattcaaccc
     2341 gcgcatggaa agccgctggc agcctggatg cctacggccg cgcaaaactc cgcgtgaacg
     2401 aattgctcgc atcctaccgc cgacctgaaa ttgatcctgt caaagaaagc agactccacg
     2461 cctttatatt ggacctcgcc caaaagtgcg gtctcgaaga attgcccaag ctcgaagatt
     2521 tccagcctgc ctgaacttct tgtaattcgt aattggcgat tggtaatcaa gagcctgtcg
     2581 caaggtgaaa ttggttttac gattcaaagg tcgttagacc tgattgccat ttaccaatct
     2641 ccaattacac acaaggagta tccatgcaac ccaaactatc cctgctcgac gatcaactca
     2701 ttgaacgcat catcgaagaa gcctatcaat tgatgctcaa gcctggtatc aaagtgcaaa
     2761 accctgaagc gaggcaattg ctcgcggatg caggcgcaca agtggatgaa gaaaccatgg
     2821 tcgtccgcat tccagagcag atcgtcagaa cggccctgca aaccgttccc cgccagtttt
     2881 tcctttatga ctatgacggc aaccccgccg ttcaatacgg cggcgatgcc gttcaattcg
     2941 acccaggctc ctcggggatc tcgatcctga ttcctgaaac cctcgaacac aaaacagccg
     3001 aaaccgccga cttgatccgt gtgatcaagg tcgcggaaag cctgccgcag tacgacgcac
     3061 aatccaccgc gatcgtttgt cacgacgtcc ccaaagatat tcatgacctg taccgtttat
     3121 atctggtcat gatgtattca aagaaaccca tcgtcacggg cgcgttcacc aacaagaccg
     3181 tgcatcaaat gatcgaaatg ctcgccatct ttgcgggcag ccatgaaaat ttacgcaagt
     3241 atccgcgtgc cgtgttcgat gcctgcccgt cgccgccgtt gatctggtca aactttggcg
     3301 cagggaatct cattgcgctg gcgcgcgcgg gtgtgcctgc tgagatcgtc tccatgccat
     3361 tggcgggcgc ggcggctcct gtcacattgt tgggcgcagt cacccagcac gccgcggaat
     3421 gtctctcggg catcaccatt caccagcttg cttgtgcagg ttcgcccatc gtttggggcg
     3481 gagcgccatc cacgtttgac atgcgcaaag gcggcacgcc ctttggctct gttgaaaccg
     3541 cgatgatcga ttcatcgtat gcgcaggtcg gcaagacttt gggattgccg actcatgcct
     3601 acctcggcgc caccgaatcc aagatcctcg atatgcaggc agggcttgaa agcggcatgg
     3661 gtgcgatgat cggcgcattg agcggcatca acatgatctc tggttcaggc atggtcgatt
     3721 cgctattatg tcaaagccct gaaaaactag tcatagacgc ggaaggcatt gcaatggcaa
     3781 agcgcatggt gcagggcatg aagattcaaa ccgaaacgct cgccactggc ttctatgaag
     3841 gagtgaactt caaaggcggc gatttcctca aacaaaaaat cacgatgaaa ctcttcaaag
     3901 aagagcaaca catgcccagc aaagtgatcg accgcgactc gatgcgcggc tggaaggaat
     3961 ctggctcaca ggataccttc tcccgggcgc ggactcgtgt ggatgaaatc ctcgcttctt
     4021 atcgccgccc tgaacttgat cctgccaaag tggcagaact ccacgcttat gttttgaacc
     4081 tcgcccaaca agctggtctt gagcagttgc ctgtggtgga agccatggaa gttgcaggtt
     4141 gaagggttta ggttgaaggc aaaatattaa aaatcgaaag cgaaaaatag gttcatgctt
     4201 aatcttgctc aattgctccg cgtcccccat gtggataatg tcctccccta cgatgtctct
     4261 cctgatggac agaggttgat tttttcgtgg aataaatcag gaaagtggga gttgtgggag
     4321 gctggagaat cagggaccag ggaattgatg cttggattgg acggcgcaaa attttctccg
     4381 cgcttctcgc cagatggttc catgctcgcc ttcgcgctgg acctggatgg cagcgagtcg
     4441 tatcacatcg caatccacga tttcaccacg aacaccacca ccgacctgac gccctctatt
     4501 gcctacgcgc atcagccaaa tgtcagttgg tcacctgatg gaaagatgtt ggcggtgctt
     4561 tcggacatga agggacaatt tgccgcgtac ctgctcccca ttgacggaag ccccgtgcgt
     4621 ttgctgaaaa atatttttca cccctgttgg gatgcggtct ggtcgcctga tggtcagtgg
     4681 atcgccatcg aatctgagtc aacggcgagt gacaggtcga tccatgttgt gcctgtggga
     4741 cgcacccgca aagggccgaa ggtcaacacg gtgcagttga aggtcaatgg cagggtgttg
     4801 aatgcacagc atcccgcatg gtcgcctgat tcaaagttcc tcgcgttctg tggagaaaac
     4861 gttgaatggc atgatatcgg attgttcaat gtggagactc aggaaatcac atgggtgaac
     4921 gaatctgtcg gagatgatac acagcctgca tggtcgcgga gcggagacac gcttcgcgtc
     4981 gggtgggttc attctgaagg cgcacagacc agcttccagt ttaagaaagg aagcgcggac
     5041 gtcgcgcaga ttcaagttgg ggcgggcgtc cattcttttc cgaagttcac tgccgagggc
     5101 gttgtcatcc tgtatgaaga ttcgagtcgt ccgtgcgatg tgtggatgtt gaatttggaa
     5161 gacggcacgt gcaagcagtt gacggattca ttgcctgccg agttgaagga tgttcaattt
     5221 gttcagcctg aggaagtttg gtatgcaggc atggatggtg tgcaggtgcc cgcgttgttg
     5281 tatcgcggtc agagcgaacg ggcggtgatc gatattcacg gcggaccgaa ttggcatgtg
     5341 caatctttgt ggtcacctgc gacgagttat ctggtttcgc gcggatggac ggtgctgatg
     5401 ccaaattatc gcggctcgac gggctatggc aaaaagtggc aaaacgccag ccgctacgac
     5461 atgggtggcg tggatacaga tgattgtgcc gcgggcgtga agtatctggt tgaaaatggc
     5521 ctggctgaaa aaaataagat cacggtcaca ggccgaagcc acggcggctt tttgaccatg
     5581 tcctgcctga cgcgatatcc tgatctgttc gcgggaggtt ctgcagtggt gccgttcttg
     5641 aattggttca aatcgcacaa ggattcacgc gaagatctgc agcactggaa cattgagaac
     5701 atgggcgacc ccgaagacaa caaggaactt tggcattcgc attcgccata tttctttttg
     5761 gataaaatca acgcacctgt gcagatgatc tgcggcggaa acgatccgcg ctgccctgcg
     5821 tcggattcga tcgcggcacg cgataaactt gtggaattgg gcaaggaagt ggagttgctg
     5881 ttatacaaag atgaaggcca tgcttttttg aatatcgaga acgtgatcga tgcagaagaa
     5941 aagagcgcgg cgtttttaga gatggtgctc aaaaaataaa tcttaatgag gagagagaat
     6001 ggagccgttg aagtttctta gcgagaacga cgtgaaggca atgcacgaag ccacattgcg
     6061 catcatgagc gaagttggac tgatctggac tcacaagcca agcctcgata tcctgcttgg
     6121 agctggctgc accataaaag ggaatcgggt ttgcatgtcg cccgaacttg taatggattg
     6181 catcgcaaaa gccaataaac gaccgaagat tcgcggacgc aatggtgtta ccaacgaact
     6241 tggcggcgga aacctgtact tccacaatct cggcggcgcg cgcgatgtgt tcgacgccag
     6301 aacaggcaca cggaaaattg ccaccgatca ggatgtgatt gacgccaccc gggttttgga
     6361 tgcactgcca aactgccaca cagtcactcc attcttcaca ccgcaggatg tgcctggcga
     6421 attgatgtct ttgtacatgt atcgtcattc gctgccatac accaccaaac ccctgcaggg
     6481 tcctggaatt caatttgcag aagaagtaaa gtatgcagtc gaaatggcac aggtggttgg
     6541 cacacctcct gaagaattga cactgtcgct ttcccctgtc agtccgttga ccattcacga
     6601 tcctgccgcc gccgccattg ttgaaatggc aaaagcaggc gtcatctgct ccaatctccc
     6661 cgcccctaca ggcggagcca cttcaccaat gaccatcaca ggcagtcttg ttcagcaaag
     6721 cgccgaaacg cttgcccttg tcgtgcttgc gcagatcgtc aacccgggtt gtggtgtggt
     6781 gtattgcgga cgacttggca tgctggagcc gcggacggga ttgatctggg gcggcgtgga
     6841 acttggccta tcttcggcag gtacggttca actcggacat tattacgggt tcagcgtcaa
     6901 cgtgtatggg ttttcaacga atgcacacac ccttgatgca caaaacgcct tcgaacgcgg
     6961 actaaatgcc gcaatccctg ctttggctgg tgcagatgaa ctttccggta tcggcgaaat
     7021 ggaagcaggc gtgatgggat cgtacgcgca aatggtattg gacaatgaac tggtcggcag
     7081 tgtcatgcgc ctgcgatcgg gcctttctgc cgatgtggag catctggcgg ttgaaatcat
     7141 cggcaatgtc atggatggaa cacgaaatta tctcggtcaa aaacacacac tcaaacacct
     7201 gcgcgctggc gaactggccc tgaccaaact tgctgaacgc aattcctggg aaacatggga
     7261 agacaagctg gaaagaaagc aaatggcaga ttatgcaatg gaggaatccg aaaggattct
     7321 gcgcgagcac caggttcctc cgctggaacc gcaacaagag aaggaacttg atagaattat
     7381 ggccgcggct gaaaaagaga tggtaaaaaa gaagtaagaa gcggatactt tagcgcaaaa
     7441 ggaaaatgga tatgttgaaa caggcaaacg caatttctga agaattgatc gaatggcggc
     7501 gcgattttca catgcaccct gaaacgggtt tcgatgtcca tcgcacggct gggatcgtgg
     7561 cagatgaatt ggagaaaatg ggatatcgcg tacaaagagg ggttggcaaa acaggtgtag
     7621 tggcagatat cggtgaaggc ggcaagctgg ttgcgatccg cgcagacatg gatgcacttc
     7681 ccatcttcga gcaaaatgaa acggactacg tttcacaaaa cacagggaaa atgcacgcct
     7741 gcggtcatga ttcacataca gccatggcgc tgggggcggc gcaaattttg gcaaaagaaa
     7801 aattcactgg ccgtgtgcgc tttctcttcc aaccttccga ggaaaccgcg gacgaagaag
     7861 gcaagagcgg cgcacaacgc atgtacgaag aaggtgcaac caaaggggtt gattatgtca
     7921 ttgcacaaca tgtagacccc accagccctg tcggcacgat tgccattgaa gcaggtccca
     7981 gcggcggcgg tgtggataca tggtttgcca ccatcaacgg caagggcggg catggagcgt
     8041 atccgcacac cacagtggat ccgttcgtac tgctggcgca tgttatcatg gggcttaata
     8101 acatctcttc acgcagaatg aatccgtttg agcctgtggc attgagcatt ggctcgatca
     8161 tcggcggcga tgcggaaaac gtcatccctg accagataaa attgaaaggc actttgcgat
     8221 tcacatcgct tgagacgcag aagcttttac gtgatgagat gcggcgcgtc tttgaaatca
     8281 ccaagaccct tggcggcgat tacgagttgg attttctctt tggcggcctg cccatgatca
     8341 acgatgggca tgtttccaag gtcatcgatc aggtcggtaa agacctgctc ggagcagaga
     8401 atgttcaccc gatgcataaa tcgctgggcg cggaggattt ccccgaattc ctgcgggatg
     8461 cccccggagc catgtatact cttggtacga tgatcgcagg acgagaggtt tacgaactgc
     8521 atcatcccaa gtttgacctg gatgaacgcg cgcttccaat cggcacagca gtgttggtgg
     8581 aaacgacgct gagatttttg aaggaataaa actcaatttg gagagcacat gcacaccata
     8641 ctaaaatcag aaaagaagga agttgtcatc ggtattgaca agccgttcgt gatgatcggc
     8701 gaaaagatca acccgacggg catcaaaaaa ctcgggcagg cattggttga caaaaatatg
     8761 gattacgtca aacagcttgc aaaacgtcag gtggagtggg gcgcggatgt gttggatgta
     8821 aatgtcggcc atccccagat cgacgaagct gcgatcatcc caatggtggt ggaagcggtc
     8881 aaatcggtgg tggatgtgcc gttgtgcatc gattcgaatg acccaaagat cctcgaagcg
     8941 ggactcaagg ttgctcccgg taagccgttg gtaaactctg tcaacgggga ggaaaagcag
     9001 cttgaaactg ttctgcccat cgttaaagat agaggcgcag ccgtcatcgg gctgaccatt
     9061 ggcgatgatg gcatcccgcc cacagcagag ttacgtctcg cggcggcggc aaaaatcatt
     9121 gagcgcgcag ccaagatcgg cattccgatc gaagatatca ttattgatcc cctggtgatg
     9181 acagtcggtc acgactcaaa agcggcgacc gttacactca agacgatcga attgctggtg
     9241 aaggaatttg gcgtcaacat caaccttggc gcaagcaatg tttccttcgg gttgcccgac
     9301 aggcattcgg tgaatgcggc atttcttgca ctcgccatgc agacgggtgt gacctgctcg
     9361 atcactgacc cgatcaagct gggaagcacg atcaaagcca ctgacctgct cctcgggcgc
     9421 gatgcaaact caatgcgcta cttgaaatac ttccgtgcga ctgaaaaact acgggaagca
     9481 gaagcagccg caaagcaata aatacggcct caaacatgga gactcacaag ctttcagacc
     9541 ttgtgagtct cttttcaata ttgtattctc gcccaagagc gcaatctgat acacttcctg
     9601 ttatatgaga actccgtttt ggttgaggcc atctcttgca agacaaatag caacccgtat
     9661 gagtttcctt gccgtgctgg cggttctcct attgacctat caatacacaa aacgcgaatg
     9721 ggaatattca caaaaggaaa tgatctcgca agccgggctg ttgttggaca cgatctcact
     9781 caccttgcgt gacccgctct acaaccttga aattgacgaa ctcaaagacc ttgctgggaa
     9841 aatcggggaa aacccgcaga tcacaatgtt cgtggtgtat gacaaaacgg ggagaattct
     9901 ggtggattca tccaaacctg agttggaatt ttcccaagaa gtggacgtgc ttggttacaa
     9961 cctggtcact acccccaccc atgaactgta tatttcatgg cagggaactc aactggtttc
    10021 aggaaaaagc attcggatcg gaaaccagac cattggagcc attgcaatcg gtttttccac
    10081 ggaaaagcat gatcaaaaag taaaagatct gactttgcaa gggctgacac ttgcactggt
    10141 tgtgattttc ctcggcggcc tgttcacatt tggcacagcc cgacagatca catctccatt
    10201 aagtgagttg gcacgggtgg ctaacaaaat gaaggcaggc gatctcacgg ttcgggcgaa
    10261 tcaaccgagg gtaaatgatg agatcggcca actttccacg gctttcaacc aaatggcaga
    10321 atcgatccaa cagcgccaga gtgaattgcg tgaacttgca aacgggttgg aaaaaacggt
    10381 caatgagcgc accgctgaac tgcaagagca ggccaagatc ctcgaagaaa tggcgatcac
    10441 agatccgctg acgcaagcat acaatcgcag gcagttctac aaactggcaa agatcgaaat
    10501 ggagcgggca caacaacaag atgggaaacc gctttcggtc atcgtgatcg atgccgacca
    10561 ctacaaacgc gtaaacgaca cgttcgggca tcagatcggt gacctcgtgc tgataaagtt
    10621 ggttgagatc tgtcacaatt ccattcgcaa gaccgatgtc tttgcacgtt acggcggtga
    10681 agaattcgtc atcctcatgc cagaaacaga ttcagagaca gcgcgagtga tcgcagaaag
    10741 aatccgtgaa agagtggaaa gcaccaaact gccagtagat ggccagcttg ttcaaatcac
    10801 catcagtctt ggggtgtcca cttactccaa tgcatattcc ttaagcttcg actctctgct
    10861 aactcaggca gatcgcgctt tatacaaatc caaacgcttg ggcaggaacc gcgttactgt
    10921 gtgggcagat gaaatgctgg ttccaggtta gaaaaatctg cttcacctca tcttgaggac
    10981 aggagtcctc agaatgagta aaaaacttta tctgtattca ctggtccttc ttcttattgc
    11041 ttgcagcaca caatcaccag cgatttcacc gccaacccct gttgccaccg ctacacaaac
    11101 cattccccca tcacaaactg cttcaccaac tttcgcatcc acgtttacac cgatccccac
    11161 cgccactcca attccatacc cgatgagcat catcgccatg cggaatggag aataccctgg
    11221 cagcgaaatc accattgtca aagaactgga tcgcggttcg aactatcgcc gctattattc
    11281 ctactacctt tcagagggtt taaaaatcta tgccctgcta accatcccaa acggcgaacc
    11341 acccgaaggc ggctggccag ccatcgtctt taatcatgga tacattccac ccgatgtgta
    11401 tcgcaccact gagcgttata ttgcctacgt ggatgaaatc gcaaaagcag gttacattgt
    11461 ttttcgcatt gactaccgtg gtcatgacgc ctcggaaggt gaacctactg gagcgtatgg
    11521 cgatcccggg tatcaaatcg atgtattaaa tgcggttgca gccatcaaac agctgcctga
    11581 agtcaaccct gataagatcg gcatgtgggg acattccatg ggcggatacc tcacactccg
    11641 tgccatggtc atctcgcaag atgtaaaggt cggtgtgatc tgggcgggtg tggtggcttc
    11701 ttatcaagac ctgctgtaca actggcggcg cacaggctca ttcactccct caccaagttc
    11761 acgtggggtg ggctggcgca caagatggat cgaggaattt ggcactcctg aacagaatcc
    11821 tgtattttgg gattcgatat cggcgacatc gtatctcagc gatctttctg gaccgttgga
    11881 attgcaccat ggaaccgcag atgaagacgt ccctttggat ttttccattc gccttgcaga
    11941 aatggcacgc aatgcaggtc aaactgcgga tttatacatc tacgaaggtg ataaccacaa
    12001 tatctcggcc aatttctcca ttgccatgga gcggacgatc aaatttttcg actcgtacct
    12061 gaaatagcgc tgcaacgtat aataggcagt atctacacag aaaacgagta caccatcatg
    12121 tttcaaaaca aaatcgcact tgtcaccggc tctggccgtg gaattggccg cgccattgcc
    12181 ctgcacttcg ccaaacacgg cgcagatatc gtggttaatt tctttcgcaa tcgcaccccc
    12241 gctgaagaaa cagcacgtga agttgaaaaa ctcggtcggc gcgccctgct tataaaagca
    12301 gatgtaggcg acctggacga tctcaaccgc atgtttgatg agattcaagc ggggtttggc
    12361 gggttggata ttctcattca caatgcagct tcgggatata accgccctgc cctggagcaa
    12421 aagccaaaag gttgggactg gaccatgaac atcaacgcgc gtgccttgtt atttgcagca
    12481 caacgcgccg cagccttgat ggaaatccgc ggcggcggaa agatcgtcag catttcgagt
    12541 gctggggcaa cacgggtact gcccgattat gtcgtcgttg gcgccagcaa agcagcattg
    12601 gagtcgctta cacgctacct gggtgtggag ttgatctcaa agggaatcaa tgtcaatgcc
    12661 gtgtcaccag gcgtcgttga gaccgatgcg ctgcatcatt tcgcatccat ggggaatcag
    12721 gacaatatcc tgcaaaactt cattgcacag gtaccagctg gaaggttgat cacccccgaa
    12781 gaagttgccg aagtgaccgc gttcctctgc accccagccg cagcgatgat cgttggacaa
    12841 accattgtca ttgacggcgg ctacacattg ccaattcccc aataaaactt acccatcata
    12901 agttgaaaat cggactccgt ttttcgtact gttcttaacc gcatacatca acttgtcggc
    12961 aattctaatg gtttccgcag gtgaaattgg tgaagcctca aacgtcactg cccctgcact
    13021 gaaagtgaca taggaatggg tttgtttcaa ttgttcggaa atatggtgat gaactttggc
    13081 gatgatcgct tcggcctcct gccgccccgc ttcaggaaac aggacggcaa attcatctcc
    13141 acccagcctc gccacaatat cagatttacg taccgcttgt tttaactcct gggcaataca
    13201 ggtaagaagc ttgtcacctt cgtcatgacc gaaacgatca ttgacctgtt tgaagttatc
    13261 caaatccaga tacaccagtg tgaacggtct tttatatcgg ctcgaacgat caagctcagt
    13321 ttttaataat tcatgaaaat agcgggaatt aaaagccccg gtaacaaaat cagtacgcgc
    13381 cagagtttgc atcttatttt gcgctcgatg caattcggac ataaggtagc tcacaatggc
    13441 aaaaaaacca aaacgaatga gcgtgttcca caaataaaca aatttgcttg agtaattttc
    13501 accaattaca aggtcggcaa tcagccaggt tacagcgccc agaatagaga atattgcccc
    13561 tgcagcggaa ctcacatacc aggcagtcaa agagactgga attaagtaaa acaaagaaaa
    13621 gctaaactca ttcccagtta gataatccac aacgcctaac agtcccacaa tgaccagact
    13681 tgtgaatatc caaaagtacg ggctccgttt ttcaagtagt gcaaaaatct tctgcatttt
    13741 tcgcgtttct gttatcgcaa cgatgaatat atttctattt tacacccact taaaattaga
    13801 catccgttac cgcatttatg cttgcggcaa cggatggatt ttttcacaaa gaactatata
    13861 ctaaggaagc gcaattggtg taagggttac cgtcggttgt ggaacagggg tgggcaacaa
    13921 aggcatccaa ccgctgtaat aaaagtaatc ggagaggaac tgcagccgtt ccgccgcggt
    13981 catcgggcgt ggacgtgcat aatcctccag tatggaggtt aacaattcaa caccgaggta
    14041 gcggccatcg taaaacacat gccgatcatg gaactcacgt cgtgtttcat caatgacttc
    14101 gccgctgggc aggttatagg tcatctgatc gaagaacatg ttgccgaggc cgtaatggat
    14161 gaatgcatca ccgtgaaatt ccatcacatg cggaaagtgc gcctgacttc cgctgacgat
    14221 caccgcaccc tcatcggcgg ccctgcggaa accttcttca tgtgtgtaac atgggccagg
    14281 tgaataacat tcttcatgct gaaatgtaaa aatgatgttg tagccctgcg ccttcaaagc
    14341 ctgcatttga tcgccaaaaa aatcaaaatc acatagggct ggcccggggc tggttgcact
    14401 ggccttcggg tatttccctt cccgttttcc attacaaccc atgaaagcga tcttgttgcc
    14461 attgacttcc atcaacacag gctggcgggc ttcctctgca ttgatgccgc cgccataata
    14521 gggcaggtta ttggccttgt acattgccag agtatccaac aaggcaactt caccacgatc
    14581 tcgcaggtga tcgcctgtca cctccaccac atccacaccc acatccaaca gcagatccat
    14641 atacttcggg tcactgcaca aaataaaatt tcgatagcct gagcttgggg tcgggcaatc
    14701 cttgtcaaag gtgacttcgt tgctgatgtg ggtgatatct gcctcacgca gttcatcgcg
    14761 cacgaattct ccggggaagg ttacgccacg caattccatc gtcactgctg tggcacgtac
    14821 cagtgcagtg acaccggtca aaatcactgt ggtgagtttg gcggggtcgc ggttggtttc
    14881 aggcaatgca aactcttcag gttgtgaaag gctgaagtga atttttagcg gataaacttc
    14941 tgcatcgaaa ctttttcgca aaggagattg accatccacg ctgagcactt tccatttggg
    15001 ttgaatctct tcaaaaggaa tgattgccca cgcggactct ttccatgctg tatcaaggat
    15061 ctcccctgcg ggaacgcttc tgactgagcc tgaagctggt tcgccccaaa cagaagcgaa
    15121 cgccctcaga gttgattctg ccaatagaac gctgtgcccg ttcaacggtc cgctggatgc
    15181 gcctttccag gtggagagta aatcttgtaa ggccacatca tctgtcagtg tgggaaaagg
    15241 cgcgacgaga gcgtaaatcc acaaagatcc agaatcagac atatccagtt tttgagtggc
    15301 gagagcaggg tcttcggtta ttggcaaccc tgaagatatt gcagcttcgt gcaaaacggc
    15361 agggaccgct ggggcaatcc acaacgctgc aggcatgggt gtattggtgg agacgggttg
    15421 tggagatgcc gttgcagttg gtattgggtt tgtagctacg gcaggcgcat tcgtggctgg
    15481 cacacaagcc gcgagcatgg caaggcaaag taagtaaaca gggatttttc tcattcagat
    15541 tccaatgacg ttgggtaggc tgagcattct acacaaaaag cagaatggac gctaggagcg
    15601 tccattcgag gtttgctact gtcccacttg caaaaagccc cactcacttt ccttgaagat
    15661 atcagtgaga aaacgggtac gttccaactc ggtcatataa cgcggacggg cgtaatcggt
    15721 gagccaggct gtgatcagtt ccacgccaag gtagcggcca tcgtaaaaga tgtgacggtc
    15781 aataaactca cgctctgtga ggcgcggtcc gcgtttggcg atctggtcaa agaacaggtt
    15841 acccaaccca taatggataa atccgccgtt gtagaattcc atttcctgag gaacatgcgc
    15901 ctggcttcca ctgacaatga tcgccccggc atcagccatt ctgcgaaaat caatgatctg
    15961 ttgcgggcgg gccttggcgg tatcaaattc gtggtattga aaagttgaga tcggtaaata
    16021 tccctgcgag cgatagtagg cgatctgctc tgtcatgtaa tcaaaatcac agggaacggc
    16081 accgggcacg gtatcggtgg cgctggcgta aatggttttg taattacaac cgatgaacat
    16141 gatcttgttg ccgttcactt ccatgagaag cggtttgcgg ccatcttcca cgttgtaacc
    16201 gccgccatag tagggcaggt tgtattgttt gtagatatcg agcgtttcat acatggctac
    16261 atcttcatag tcagcgaagt gatcgcctga aagctcaatg acatcggtgc caatatcggt
    16321 caacaattcc atgtagcgcg gtgaactgca gaaaatctgt ttctcctgat ttgggtctgg
    16381 tgaagggcag cccgtgaaaa atgggacttc gttgttgatg tgggttacat ccgcttcacg
    16441 cagcaagtcg cgaatctctt ctccggggta ggtgatgcct ttcacgtcca tcgtcttcgc
    16501 tgttgcacgg accatggccg tcacaccggt catgatgacg gtggcgagtt tgttcgggtc
    16561 acggttttgg taggagaaat ccaattcggc tggcacttcg caaggctcag cacaggtcag
    16621 ccgataggtt acagtcaacg ggtaaatgac gggatcaaaa tgcttttgaa tgggagattg
    16681 accgtcaatg gtcagtacct tccatttggg ttcgatcttt tcaaatggga tgatggccca
    16741 cgatggcatc tggttccagg ccgtatcgag caactggtct gcgggcacaa cttgtacgct
    16801 tcctgctgca ggtgcgcccc acagcgcggt aaaggtcatc atggtggatt cttccatcag
    16861 gatcggaatc ccagcaaagg gtccgaagga agcgccactc caaaccgaga tcaattcctg
    16921 tgatgagagt ccatcaatga tggttgggaa cggtgccacg agggcatagg tccaggtgga
    16981 tgtgtcaaat gcacctgtga tgggttgagc aacgtcgatc tgcaaatttg ccgaggcctg
    17041 attcgtagtc aacacttgaa agccccagtg ctgtaatttt tcacgcaagg ccaatggtgt
    17101 ggcagggctt gcccaaacct gggcagcatt caaaaccacc ggggttggaa cagcggttgg
    17161 cggcatgggg gtaatggttg gcgcaagagt tgggatgcgg ggcatcgccg aaggagttgc
    17221 tgcggcaaag gttggtggcg gcggtggagc cccccacaaa gagggctgac aggcaaccag
    17281 tgacaaaatg ctgatcaaga gaaaaccaag gcgtgtggcg cgatgtccat tccagttggg
    17341 ttgtcgtaca aaaaaggagg atttaattaa agtaaatctc cctgccagat atgattcaag
    17401 tcgttccaaa aaaataatgc gaggcatctt catgtctcgc attatatgaa gcaaacgctg
    17461 tgcgcttcaa aataatatca atacagtttc cttaatcatg aatttgcaac gattgaaagc
    17521 gttgcaagcg aaaacagttt gagcgcttct tcgtcaatgg tcatgcccag gccagggcca
    17581 ttgggcaccg tgatcgtgga gtcggggttc agtacaaatc gttcatttgt aatatctcgt
    17641 ttgtaatagc ggtctgaagc agaaacatcg cctggcaaca caaaattagg cagggaagcc
    17701 aatgccaggt tggaggcacg gccaatgcct gtctccaaca taccgccaca ccaaacaggc
    17761 agggattttt ccatgcacat atcatgaacc atcaccccct gactgatccc acccagcctg
    17821 cccgccttga tattaatgac acgacacgcc ttcatctcaa tggcgtaacg cgcgtgacgc
    17881 ggggaaacga tgctttcatc aaggcagacg ggcgtctcaa attgctcctg gaatttatga
    17941 tgatcccaaa tatcatcttc aaacaagggc tgttcgatta aaagcaaatt cattttatcg
    18001 agcggcttca acagaggggc atcatccatc gaatacgctg aattggcatc cacttgcagg
    18061 cgcaggtcgg ggaatgcttt tcgaacggcg gccgcatctt ccacatccct gcctggtttg
    18121 atcttgatct tcacccgggc atacccctgt tcaatgaatt ccgccgcggt cttgaccaac
    18181 ccctgggctg attcttgaat cccgatcgag actcccactt caactttctc tcgcgctcct
    18241 cccagcatct cgcgcaggga tttgccagag cgcttcccaa gcagatccca gatcgccatt
    18301 tccacgcctg cttttgcaag gtgatgtcca cggatccctg ccacacgcgt ttgaaaatcg
    18361 agggcatcct tcacatccac accgaggatc aacggcagaa taaaatcctt gaggatatga
    18421 atggcagtgc cggtggtctc gtagttgtaa cccgggtcac gagaggccac acattcgcca
    18481 tatcctacca acccttcaga acgcagttcg atcaacacac attcgcgatc tgtttcgcgg
    18541 ccaaaagagg tttcaaaggg ggcaacaagc ggcatgctta agtgatgaag tacagctgag
    18601 tctattttca ttttggaatc ctttattttt atataatgcc ctcggtcaca aattgcactc
    18661 tcccgcttct aaatttgtga ccgtgacggg tataacttca agaccacgaa acctttgggt
    18721 ttttgataca cccgtctgat ttcgtggtct tgaagatttt attattctat taccaaaaac
    18781 agatcaccct cattggccgc ttcaaccagc agaatcgact tttacttctg ttgagtcatc
    18841 cgtcagcttt aagttgcgca acgcatcatc catgcctgtg gattcaaggc gcatccccgg
    18901 ggcgggtgtg ccgcaaaatg gacagatatt ccagggtaat tccataaatt ttccacagtt
    18961 gtgacaggac tttttcaatt tggtatggca attcgggcag acctgccaat cttctttcac
    19021 gcgccgttca cagccagggc agagcggaag gtcttccaag gcctgcaaca gggcttcttc
    19081 ttccaaggtg cgttgatatt cctcttccaa agtgcgcggc gggcgcagaa tcaaatacac
    19141 aaggaccccg ggcaggttga ggactgccac caacaaggcg gcgagcgttt gtgtcaaagg
    19201 gtcgcgggca cgggcacgga tatcccgata cgtccacacc acaagactga tccacaacgc
    19261 ggcaagaaat gcgccgccaa aagcggtcag gacaagggtg aggcttccaa ggaatacggg
    19321 gtcaaaggtc atgtcaactc caaacgaaca cggatgcagc gtattttatc gcttgtcaca
    19381 caaattggta aactttttct catggggttt caaatttgaa atgccagtca ctgccatcct
    19441 gggtgtaggt tgaagtaaaa cccatcactt cgtaaaaggt acggcccggg cgcaggtaga
    19501 tcggactgcc atccaaattg gtaagcaaaa gcggctggtt gatatctgtc cgctcccatt
    19561 tggcagggac aacaaatcca tcccgaaaca cgaaggcatt tccataatcg atcaggtcaa
    19621 tgttataaac ttcatctttt gcgttgaatt cattggcaaa ggtatgcggc acaaaaatga
    19681 caacgatgtt ctctgcattg accggtaaac cggtctcggc atcagagagg ggtgcgtagg
    19741 cttcgggttt gccgttgagc atatcgttgg cttcctgata gcggaaatat ttgtgtgtga
    19801 tcacatcata ttcccaatag ttgtagctct gccttgaata ataggaaaag atgcgtgaag
    19861 ctgtggttgt gccttccatg ggggcatcgg agaagaatcc gccgcgaatg acttgcggag
    19921 agttatcgag tcctttgctg gcagcgcagg cggcccattc ggcagtgaca aaaaagaaat
    19981 tgttgtaatc atcccgtttt ttatcggggc caaagatgaa tggcgggcac cctgaaccgc
    20041 ccggcacaac cagaaactca ttcagagtgg aatttttcag gtaggaatat tcgcgcgggt
    20101 cggcaaactt gaaaaccaaa aaggcgttgt acatgcgggc gacgtgttca tcaaaatatc
    20161 ttcctgaacg caccgggcca acttttggcg agtcgtttcc atacatcacc gcaatgaaac
    20221 gtgtatcacc ccattcgatg taatattcat aaaccacgtc tgccagggtc agcccggatt
    20281 gcgggcggat ggtatctggc gcgttggcta ctttgatcac catcggcctg cgttcaagca
    20341 gggctgggtc gttgacgggc agcccggtca aggggttgtt gatgttcatc aagctgaaat
    20401 caccagcact gccgttggca gatgggtcca tcaatagtgg tgtgggcagg atttcgatgg
    20461 ctgtattctg tcggggaaga ggggtgtacg tgggttcggc tgggatggcg gcaacatcgg
    20521 gagtggaagt tgcttctggc aggggttgaa aaggtgtcag cgttggggcg gccaaaggaa
    20581 cagaagcagg cagagggtcg ggagtggaag cgcatgaagt cgccagaaaa aatgccagca
    20641 gagcgagaat ccgcctttga agaatcatgt ttatgattgt accaaatggg aaacagggaa
    20701 agtacgtcca caataaaact gatacaattg tttactccat gctaatctta atttcctgct
    20761 tgttgctttt tttaaccgca ctgatcctgc ttgttttagg tgtgacccag ccaaacgcgc
    20821 ggtacacctg gcttgtggcg atcggcgggg caatgctttc gttggccagt gtgttcatct
    20881 ggctggcgcg catcccagtg gatttcacgt tgcccgcctg gcagccattg accttgttcc
    20941 aaaacccgat cctgtttcgg gcggatgcga tctcatggcc ttatgcggtg gctatctctg
    21001 gcatcaccct taccatcctg ctgactgagg taacgcggcc caattccacc agtttgtatg
    21061 cgtgggcagg tgtgcttgca ctgggcggtt tgggaattct ggctgttact gcaaacaatc
    21121 cgttcacttt gctgctgatc tggagcgcac ttgacctcgc cgaactcatc acccagcttc
    21181 gttcagtgga tgggccttca aacaatgaac gggtggttat ttctttttca acacgggcat
    21241 ttggcacatt tctgctgctg tgggcaaatg tgatcagtat tgcacaggga agcacattcg
    21301 attttcattc catcgccgca gattctggtt tgtatcttgt ggctgccgcc gggctgcggt
    21361 tgggggtttt tcccttgcac ctgccttact catctgaatc cgtgttgagg cgcgggtttg
    21421 gaaccgcttt gcgtttggtt gccgccgcat cgagtttgat attgctggcc catgtgcctg
    21481 ccggcagtct aaattcaagc gtcacaccgt ttttgctttt atttgccctc atcgccgctg
    21541 tatatggcgg ttggatgtgg ctgcgtgcgc ccgacgaatt aacaggccgt ccatattgga
    21601 tcatcggcat cgccgcgctt tcaattacag caaccttgaa tggcaatcca cttggggcaa
    21661 ttgcgtgggg atgcgcactg attttggtcg gcggggcttt gttcctttct tcggttcaac
    21721 acaaatggct gagtcgtttt ctgctggttg gagcatggag tctttcatcg cttccgtttt
    21781 cacttacggc cagtgtctgg cttggcaatt taaacttctt cgttccgttt ggagttgcgg
    21841 cacaagcctt gttgaccgca ggcctcattc gccattcatt gcgccccggt gacagaggat
    21901 ctctcgactc tcaggagaat tgggtcaacc gtatttatcc agcggggatc atcctgcctg
    21961 cagtgcttca gattattctg ggattgatcg gctgggacgg tgcgcgccag atcggagcct
    22021 ggctgctggc ggtgatcgct tcgctcttaa tcctcgggct ggtttgggcg acaccacgtt
    22081 ttcgcatcct caacccgatc cgtgctcatt gggtgaaccc cattgctgca aataccccca
    22141 gcgtcttcta cagcagtttc tgggcggtat accgcgtctt tgaaagaatc aatctcacga
    22201 tcacgaccgc actggaaggc aacggcggcg tgatgtggac cctgctcttc ctggtccttt
    22261 tcatctcact tctgacacag gggctcccct agcatggcaa ccgatatcat ttcatggatc
    22321 gcagtgggaa tgattctcgc aacctctgca attatcttaa tcagccgtga ctggcgtatc
    22381 ccgttgggcg cactggcttt gcaatacctg gcagctttct ggctggtgac tcgacatctg
    22441 ccctttgtaa tgggctctgc caaactcatc acaggctgga tggtggtcgc ggccatcggc
    22501 atgacccgtc ttgggttgac agccacagag gaaaaagccg aagatacgtc tctgcaaagc
    22561 ggacggtggt tccgcggcat tttgatcggg atcgtagctt tggtcacggc tggggcaaca
    22621 ccgcgtatcg aaacgagcat tccaggcctc ggcctgcctg taatcgcagg cagtatcttg
    22681 ctgatcggag caggtgtgat ccacctcggt gttacctcgg atattcttcg agtcaccctg
    22741 ggtttgctga ccatgctgac aggtttcgag gtactctatg ccgccgtgga aagttcaatt
    22801 ttggtggctg gtttgctggc attgaccaat ttggggttgg gaattgcagg ttcctacttg
    22861 ttgatcgcag gttccattcc gctggaagtg gaggatgaag aatgagcgcg ccgatcattt
    22921 ggatcctcat cccatttgtt gcaggggttt tgattctttt cctgttacaa gagcgtattt
    22981 cctcctttgt tggcggggtg gtatccatgc tgctggcatt gaccgccttg atctttccca
    23041 tcgataccgc tttactaatt ggctctgttt ccatcaaaat ttcttccacg gttcagtttt
    23101 tcgggcgcag ctttgcgctc aacactgcgg atggtccatt gctggccatt atttatggtc
    23161 tggcgacctt gtggtttttc ggcacccaag cctctggcac agccaaccgc tttgtttcac
    23221 tcgggctgat gatcgtagcc ctgctgaccg cttccattgc agtggagcca ttcctgtttg
    23281 cagcactgct gatcgacatg gcagccatgt tggtgattcc actgttggtt ccactgcacc
    23341 aaagaccagg tcaggggatt gttcggttct tgatccagca aacgcttgca atgccgtttg
    23401 tcttgttttc aggttggatg ctcgcaggtg tggaagccag ccccggtgac attggcaaca
    23461 ccatccaggc aggtaccatg ctcagcttgg gattcgcctt tctattggcg atcttcccgc
    23521 tatacagctg gataccgatg ctgactgaag aagcctcacc ctatgccaca ggtttccttt
    23581 tgtgggctct tccaaccttc accatcatat ttttgctaaa ctttttagat cgctacacct
    23641 ggctgcgaag cacacctcag ctttcaaatg ccattcaatt tgccggcgtg ttaatggtgg
    23701 caagcggcgg acttttcgct tcacagcaaa agcatatcgg aaggatgatg ggctacgcct
    23761 ccatcgttga gaccgggctg ctggtccttg ctgcagggtt gaaatccacc gaagtcgtca
    23821 atgtcacctt cttgctgctc accccacgcg gattggagtt ggcggtttgg gcacttgcgc
    23881 tttccatcat aaaacgaaaa gtatattcac tgcgcttcag cgaagttcgc ggtatggccc
    23941 gccaataccc cattgcagtc gcagccatcg ttctggccca tctttcgatg gcaggctttc
    24001 cgctactggc aggcttccca tcccgtgcgg ccctgtggca ggaattgaca cctgaatcat
    24061 tgaccatgtc gttctgggta ttgatcgggc tgttgggctt gttgatggga gccatgcgta
    24121 cgctggcggt ttttgtcatg gcagaagaaa atcgaacctg ggagataaac gaatcctggg
    24181 ttcagatggg catgctcggg gtcggggtga tcgggctttt catcctcgga attttcccgc
    24241 agaccctgca acccttcatc gccagcctgc ccgccctgtt tgaacacctc gggcaataac
    24301 ccgttttacg ggcgtgctat aattttgcaa atttccccac agccatcgga gagataaaaa
    24361 atggaattcg aacaaatcat taaacgcctt gaatggttgg ataaacaaca gcgcaaaaat
    24421 caggatgccc tgacaagcct cgacgggcgg ctggagtcct ttgaaacgac catcaatgcc
    24481 gtctcaaaac aaatgaaagg gttcaccaaa cagatcaccg atgtctctcc catggagaag
    24541 cgccttgacc agtttgaaac cctgatgacc cgtcaacgcg cggaatttgt aaaactgctc
    24601 gaagagaacg aaaaagcgca aacccgcact acaaaagaaa atctgaaata tgtgcaaacc
    24661 gagttgaccg agatccaaaa ggtcattgcg aacatcaaaa caacgaccaa cctgaccgag
    24721 atcaaaaaac aactcaaaga acgcgccgat gaaatgcagc gagtcttgaa caatgtagcc
    24781 gatttgaaat tccgtgtgga tgaagccacc cgcacaaaag acgacgccgt ccatgcttta
    24841 aaggccatcg aagaaacccg caagaacgac ctgaaacgcg tcgctgacat tcagggtgaa
    24901 gtgaccgccc tgcgcaaacg tgtggatgaa aaccgcgaca aagccaccat caccgccgat
    24961 agcattcgca atgttgaaaa tcgtttcaca gacctgatcg cctccgaact cgaacgcaag
    25021 caggcacagg ccgccttcct ggaacaacaa accctcgcac agatcgaccg tgaccgcgcc
    25081 tggaaggaat ggaaggagaa atacgaatcc ttccaaaaag aagccgccaa ccttgatatc
    25141 cacattcaaa atctggacga agccctgcgc ggagcaaaaa aagcgcagga aacctacctc
    25201 gagctcaaca caaaactcga acgccgcatc aacgaagtga ccgaaatgca gcgtctcgcc
    25261 gaagaccgcc tgcgccagga atgggtcagc ttcaagaccg acgaccaaaa acgctggaca
    25321 ggctatagcc tctcctccga agaatctttc cgcgatattc gcaaggaagt gcaaaaactc
    25381 gaaacccgca tcaccccctt cgacgacatc acccaggtat tgcaagacca aatgcaccaa
    25441 accaccgatg ccacagaaaa gcaattgcag gaaatgatga acgttctgca tgaatggatg
    25501 ctttcctatc agcgcatcat ggggcacggc aaaaaatcca aaaaataaaa caactctttc
    25561 ctctttcttc tttcagcatt caaagaaagg ggaaagaaga aagaaccaca ggcatgagag
    25621 tcattggaac ggctggccat gtagaccacg gaaaatccac gctcattgca gcacttaccg
    25681 gcacccaccc cgaccgcctc aaagaagagc aagcccgcga aatgaccatc gagttgggtt
    25741 ttggctggat gaacctgcca aacggcgaag aagttgggat cgttgatgtg cccggtcacc
    25801 gcgactttat cgaaaacatg ctgtcaggca tcggtggaat tgacgccgcc ctgctggtca
    25861 tcgctgccga tgaaggggtc atgccgcaga cgaaggaaca tcttgccatc ctggatctgc
    25921 tgcaaattcc cgctggattg atcgtcctga caaaaacaga cctcgtttct gactcaacct
    25981 ggcttgacct tgttgaaaca gacattcgcg gcgccgtcag tggaaccgtc ctgcaagatg
    26041 cgcccatctt gcacgtttca gcaaaaaaca aattggggct caaagaacta acggccagcc
    26101 ttcaaaccct gctcgaaaac aaacccgccc gccccgacct taaccgtcca cgcctgccca
    26161 ttgaccgtgt attcaccatg agcggatttg gcacagtggt cacgggtacc ctcagtgatg
    26221 gtcacctcac cgtcggcgat gaagtagaaa ttctgccctc gggccagaag ggtcgtgtgc
    26281 gcggcttgca aactcacaag aaaaaagaag agcaggctct gcccggctcg cgtacggcag
    26341 tgaatatctc cggggtcaac actgagcaga tccagcgggg tgaggtggtc tcacacccaa
    26401 accagtacac acccacccgt agaatcgatg cgcggctgcg gttattgaaa gacatctcca
    26461 aatccatcaa acataacgac gaagtaaaat tcttcacagg cgcaagcgaa acgattgcca
    26521 ctctgcgcct gcttggcgcc gaagaacttc gtgaaagtga agaaggctgg atccaactcg
    26581 aattgcgtga ccccatcgtt gctgtgcgtg gtgaccgtta catcctgcgg cggccgtcac
    26641 ccagcgaaac ccttggcggc ggtgtcattg tggatcatca gccaaagggc aggcacaaac
    26701 gctttgacga aactgtgctc aaatcgcttg aatctctcgc acagggctcc ccagcggata
    26761 ttctgtttga agcagcgctg gccctaaata ctgcttcggt gaaagaaatg gttgcccgtt
    26821 cgcgtcttga atctgcacct gctggtgaag cattgcagga gttactggca gatggcaggc
    26881 ttgtcctttt ggaagaagga gaagcaacag ccgcaagcga cctgcttgca atagccattc
    26941 cgcactggag catattgcag gagaaaaccc ttcaactcgt taaagcgtat caccagcaat
    27001 ttccattacg gcgtggaatt ccacgtgaag agctaaaaag caggctaaaa ctcactccac
    27061 gtgtctttaa tgccacggtc aattttctca cccgccattc agcgctgacg gatttgcgcg
    27121 gttcggtggc acaaaccacc catgagataa aattcaatgg acaggatcaa gcaagggtta
    27181 atgggttgat gcgtaaattc gaggcgaatc cgtttggtcc gccaagcgtc aaggaatgtc
    27241 aatcagaagt tggtgaagag gcattcaatg cgctgcttga aatgaacgaa ctggtgagcg
    27301 tgtcaccaga tgtggttttc aaaaaacaag accatgaact gattgtgatc aaaattcgag
    27361 ccgagctgaa gaacaaagga acgattaccc ttgcagaagt gcgcgacctg ctcggtacaa
    27421 ccagaaaata tgcacaagcc atcctcgaat acatggacgc cattaacatc accatccgcg
    27481 acggtgatgc gcgcaaattg aggaactaaa agcatacctc caattttttt tatgggtaac
    27541 tcattcaagc cacccacttt cgatagcgaa gaaaagtctc accaggcatt cttgttgcgc
    27601 gccattgtgt ggggcatgat ccttgtccca attccctatt taatatttat agcgatcacc
    27661 aatccttcgg gattcaatcg ggtgctggcg caatcagcct ttggagaggc gatcaacctt
    27721 ttgcttctgt tcatgcttag aaaagggatc gtccgcctgg cagccatact ccagattgtc
    27781 atgctatgga tcttcttttc catcactgcg gtttccggtg aaggggtgca gagcgaagct
    27841 tacttaatgg gattcccgtt ggtaattttg attgcggggg ttcttttcgg cgggagagca
    27901 gcctcgtacg tgacggtagc ctcgctcttg actgggttgg tattgatcta cgcagagacc
    27961 cagcaggcca ttctttcaac aaatctcacc cgctccatga atatgtgggt gataagtctg
    28021 gttatttttc cgatcattac aacactgcaa tacctttcca gtctttctgt gcaaaatgcc
    28081 ctaaaacgtg cccgcatcag cgaacgaaaa tacatgctgc tttcaaacat cagcacagac
    28141 tatgtctttg aaaccaaagt ggatgaagca gggaacaaca ccctgttttg ggtaggcggc
    28201 gcatttgaaa aaatgacagg ctacacgctt gaggaatacc ttgcagcagg tagctggtcg
    28261 gcgcacgttc acccggacga catcgaaaaa gacattcggg atatggaagc acttgccaga
    28321 aacgaggata taaaatctga aatccgcacc atcaacaaaa atggtgaaac gcactgggag
    28381 cggatctacg cccacccgat ctgggatgat tacaacaaca gactggctgg cattatcggt
    28441 gcagtgcagg atattactga aagcaaacac gccgaagaaa ccttgatcgc aacactcagc
    28501 caaaccgcag aaatggttcg gaagatcccc gatatggcct ggttgaagga cgtgaacagc
    28561 aaatacgtgg ccgtcaatga aaaatttgcg tccgttgcgg ggcggcccat ggaagaaatc
    28621 atcggaaaga gcgatgatga aatatggaac ccggagtttg ccaaaaatta ccgtgaagat
    28681 gacttgcttg tgatcgaaac cctgaaatta aggcgggtgg aagaaaagca aatggaccgc
    28741 cttggcaggg aatattgggt ggaaaccatc aaaaccccca ttttcaatgg caagggagag
    28801 gtcatcggca caaccggggt ggcacgcgac atcaacgacc gcaaagaagc agagcttgcc
    28861 gaaaaacgcc gccgcgaaat gctcgaaaaa gtgatcgaac tcggcaaact cgtcaccgaa
    28921 gccagcgaca tcaaagggac cataaaaaag atatggcatg gcgttcacga cgatctgcaa
    28981 tttgaccgcc tggcgatctt cctatataac gctgaacgca accagatgga tgatactctg
    29041 ggaaccgaca taaacggcca aatgacagat aactacggac tgtccttccc tgtaggggaa
    29101 gacacagacg gctcgacttt tgcctacctg ctcgaaaaac cagatggttt ttacttcacg
    29161 aaagattatg ccaaagagaa taacattcct cccggacatg aaatgtacca tgtcaaagag
    29221 tacacagctg tggcggtttg ggcgggcaac aaacctgtgg cggttatctg tgcagataac
    29281 cttatcaccc tccgccccat cacgaacgaa cagctcgaag gtctgcgttt atttgcaggg
    29341 tatgcagggc ttgccatcga aaattcacgg ctgcattccg ccattcagaa tgaattagac
    29401 ctgcaaaaaa gtgcggaaca gcgtgaggct cgccgtcgtg caaccctgga aaaagtcatt
    29461 caacttgggc aaagcatgac cgaggtccac gacctccgag ccaccctcga aagaatttgg
    29521 cacggagtcc atgacgaact ggcatttgac cgtcttggaa tctacatgta caaccctgtc
    29581 aggaagtcaa tggatggcac gtttggcacc aacgaccgcg gagagatggt tgacgaatgg
    29641 gaaacctgga aatccattga ccagggaaac gaggaagcaa attccttttt gtatctgctc
    29701 aacaatcctg attccatcta tttaactcgt gattacgaat cagagcacaa gtctgccata
    29761 aatgacataa tgctcggcgt taaagacttt gccgctgtcg ccgcctgggt cggggaaaaa
    29821 cccgtggcag tcatctgtgt agaccacaaa atcagcggac gccccatatc tgatgagcag
    29881 cttgaagcct tgcgtttgtt tgctggctac gccgggctcg ccatcgaaaa cgccaggttg
    29941 aacactgcgc tacaaaacga acttacccaa cgcaagacct tcatcgaaga acttgaagca
    30001 aagaacgcgg aactggaaag attcacctac accgtctctc atgacctaaa atcgccgctg
    30061 gtcaccatca aagggtttct aggttatctt gaacaggatg ccctttcggg aaacttcgac
    30121 aaattcaagc aggatgtctc gcgcatcgaa accgcagtag gaaaaatgca aaacctgctc
    30181 agagacctgc ttgagctatc gcgcattggc aggttgatca acacacccgc cgatatccaa
    30241 tttgatgaaa ttgtcaaaga tgcactggaa atcgtccatg ggcagattga aggaaaaagc
    30301 attcacatta ttcacaagca aaccggtatc atgatccact gcgatcgggt acgtttgacg
    30361 gaagtgctgc aaaacctgat cgaaaacgcc atcaaattca tggataacca accctcgcca
    30421 cgcatcgaaa ttggagcttt cgaaaacgaa cgcggacagg ccatcttcta cgtaaaagac
    30481 aacggcgcag gcattgcgcc cgaattccac gaaaaaatat tcggcctgtt caataaactt
    30541 tccaccgaat ccagcggcac cggcattggc ctcgcgctcg taaaaaggat catcgaagtg
    30601 catggcgggc gcatttgggt cgattctcaa attaaccggg gggccacttt ttattttacg
    30661 ctttcataaa aaaaggagct taccatgtgc ggacgattta ccctgacggt tgaccctgca
    30721 gagttacaag atactttttc tgaattcacc tttcccaccc agttttcacc gcgatttaat
    30781 attgccccaa cacagcccgt cctcgccatt ccaaacgacg caaagaacaa ggccgatttt
    30841 tttctctggg ggttgattcc ctcatgggca aaagacccat ccatcgccaa caaattgatc
    30901 aatgcgcgcg gtgaaaccat tgccgaaaaa ccatcttttc gcggtagttt taaatacaaa
    30961 cgctgcctca tcctcaccga cggattttac gagtggaaat cacaacctgg cgcaaaaacc
    31021 aaaacgccct acttcatcca catgaaagac cgcaagccgt tcgcattcgc aggtttgtgg
    31081 gacgaatggc attctcccga tggcggctcg gtccgcacct gcaccatcat caccactgaa
    31141 cctaacgaac tgatgtccac cctgcataac cgcatgcccg tcattctgga ttctcaaaac
    31201 tatgccgatt ggctggacgc tgcgcctcgg actcccgata gcctgctcca cctcatcagg
    31261 tctttcccag ctgacaaaat gtccgcctac cctgtttcga ccatggtcaa tgctccaggc
    31321 aatgaccgcc ccgaattagt ggttcccgtt caataaacac ccgaattctg gttaatacag
    31381 gattagcgtt taaaaggcaa cttgactgtg attttttaag catgttatga tgaacaatat
    31441 gcaaaacgat tttaatgaag attcaacaag ctcggcgaag ccaaaggaag ccaaacaggc
    31501 tgagcctgta agacagcgta gttttttcac acggtggatg aatacactgg ttcaaatggg
    31561 gctgggagaa tcattgttac gcagtggaac aaaccttttc tccattgcgg ccatcgttgt
    31621 tgtgatcttg cttgtacagg cattcaacac ccaacgggcg aatcctgctt caggaaacag
    31681 cgaccaggct tcaggtcccg ccccgaccgt tgaagtggat atccattcga tccccgcatt
    31741 ggaaaatcca ctaattaccg ggatttcccg ttcggcacag atacatacaa acattccctc
    31801 acgaccacgc aatgagatca caaactatac ggtgcaagag ggtgacactg ttttcggcat
    31861 tgctgaaaaa tttggcctgc aacctcaaac cattctttgg ggtaattaca acattttatt
    31921 ggacgatccc cactcactaa agcccgggca ggagttgaat attctgcccg tggacggcgt
    31981 gtattgggag tggctggggg gcattccctt tggcgactgg gctgagtttt acggagtcac
    32041 cgctgcggat attattgaat acccgagcaa caaaatagac ccgaatgcgg tcggcgacta
    32101 tgcaaacgca aatatcaaga ccggcacatg gctgatcatt cccaacggga aacgggagtt
    32161 tgttaattgg agcgcgccac tgggtgtgac acgtgaaaac cccgcttctg cccgtgtact
    32221 tggtcagggc gcctgcgaac cggtcagcgg tggcgcggtt gggtatggca cttatgtgta
    32281 cccaacgaac aaacatttcc tctcaggttt tgactacaac gaaaagacaa atcatcgcgg
    32341 tattgacctg gcaggcagcg aaggtgaagg tgtgtatgct gccgacgctg gtgtggttgt
    32401 gtactctggc tggaacaact acggttacgg caatatgatc atgatcgacc atggaaatgg
    32461 ttttcaatcc ctgtatgccc acttgagtgc gatctataga ggttgcggac agagcgttgg
    32521 gcagggtgaa gccatcggtg cagtaggttc caccgggcga tcttcgggtc cacacctcca
    32581 ttttgagtta atgaccgcca cctggaaggt caatccctgg gattatttgc cgccgccgta
    32641 agtccttgcc aaaggcgcaa ggctatacta taatgcgggt cgttccgggc gcgtagctca
    32701 gttggttaga gcgcatgtat cacacacatg aggtcggggg ttcgagcccc tccgtgccca
    32761 caaaaaaaac gactgccgaa aatggcagtc gttttttttg tgtatatcca tcatatgacc
    32821 aatatttcaa ttacaattct gtgaacaaaa gggtcttcaa gtacatgtat aatcaggcac
    32881 atctttcttt gaaaggattt attcattatg aaggttgcga tcctggcagg tggattgggg
    32941 acgcggttat ctgaagaaac gactgtaaag ccgaaaccga tggtcgaggt tggcggaaag
    33001 cccatcctct ggcatattat gaacatttat gcagcatatg ggttcaaaga attcgtagtg
    33061 gcactggggt ataaaggcga gatcatcaag gattattttc ttaattatca ctacagatca
    33121 cgaagtatta ctgtccaact caaaaccggg agagtaaccg cccacaacat cgaaggcgaa
    33181 gactggaccg tacatctgct ggatacaggt gccgacacca acactggtgg acgtgtcaaa
    33241 cgtgtggcag agttcattgg caacgagacc ttcatgctca cctatggtga tgg
//
LOCUS       DAOQLC010000039        31927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_439_length_31927_cov_24.639088, whole genome shotgun sequence.
ACCESSION   DAOQLC010000039 DAOQLC010000000
VERSION     DAOQLC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 31927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_439_length_31927_cov_24.639088"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..>1114
                     /locus_tag="PL000_08840"
     CDS             159..>1114
                     /locus_tag="PL000_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC89046.1"
                     /translation="MTIDNRQFSSREQETINLLLQGKSNKQIALSLNVSISTVEFHLK
                     NIYRKLQVKSRTEAILRLGKPTGDDESRTQGQSIGSKVDKQRESIVDKISVDAENGGN
                     TNSHRRIPMKQSSYIVGGSLLFLIVLAAIAIFVKVYGHTLSAPPTTQAPLSTATMQPA
                     IVSTTAPATPESITATCPVETVDLKLFTNTNDGYCFLYPIENTAFPPNKVVINPNGIS
                     GGDFLPGDFYVLIQVEAASGRTTTQVANEKIAEAGGVLPITLTSILVDGKEAAVIDGL
                     PSQDSTRHVFIIANDRLYTFFFTPWDPRIEGFSQSLLKLYSTV"
     assembly_gap    1115..1214
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1215..1714
                     /locus_tag="PL000_08845"
     CDS             <1215..1714
                     /locus_tag="PL000_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89047.1"
                     /translation="FMAFLFLAKLIMIPLRGFKIKKEFDKPLYPIVIFTFLFLTASFF
                     DIIVIPKLWVEKFGLSTEGVVVDLRATHFRRRSYVATYEFDKDSLRFVKTQSISSSMY
                     EQLETHPKAEIRYLPINPDISYLRDLEYLKMNTFVSLVSGIGIMILLYSYKPAEDFNH
                     TSDVK"
     gene            1836..2300
                     /locus_tag="PL000_08850"
     CDS             1836..2300
                     /locus_tag="PL000_08850"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC89048.1"
                     /translation="MTKTIVSPKSESAQNAASVKEFIASLDEQTLKDSKALIAMMQGI
                     SGSKPRLWNVGTIGFDSYHYKYDSGREGDSFIIGFYPRKGKITVYLMDGIARYSDLLA
                     RLGKHTTTGYCVYIKHLGDVELPILEQILQRSYENIKSQDGHINQILWKTEK"
     gene            2395..2904
                     /locus_tag="PL000_08855"
     CDS             2395..2904
                     /locus_tag="PL000_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89049.1"
                     /translation="MEFSTIYYELQQSTEMIRALLTGITQEEARVKPNAEAWSILEVV
                     CHLHDVEREDFREHLDCILHRQDEKWHEIDPQSWITSRKYNEQNFAEMQEKFFAERKK
                     SLEWLGGLQNPDWEKVYTTEYHAFSAGEMFSCWAAHDNLHIRQLVELRRLRLEHVTKP
                     YNIEYAGDW"
     gene            3020..3376
                     /locus_tag="PL000_08860"
     CDS             3020..3376
                     /locus_tag="PL000_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HNC89050.1"
                     /translation="MNNKSNARVYAMKFSGVYPLYVQKAERKSRTKEEVDKIICWLTG
                     YSEAELRQQIEQGNDFETFFAQAPALNPNRSLIKGVICGVRVEDIEDTLMQNVRYLDK
                     LIDELAKGKAMEKILR"
     gene            3623..4168
                     /locus_tag="PL000_08865"
     CDS             3623..4168
                     /locus_tag="PL000_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC89051.1"
                     /translation="MTTPTDRDLILQTRRGDADSRTAFGELVTRYQTSVFNVCYRILH
                     ERGEAEDLAQETFIRAYDRIHTFDIEREFGPWIRRVAANLCLNTIESQKVTSELDDER
                     DADSAQLPEAVQEVRERSEQIRHALASLPVHYRAVVELRHFQELSYEEIAAELNIPLS
                     DVKSHLFRARKILAEKLHASH"
     gene            4155..4703
                     /locus_tag="PL000_08870"
     CDS             4155..4703
                     /locus_tag="PL000_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89052.1"
                     /translation="MHLTDEQLNEYLDNETNERMQIETHLSSCGECAARLSALQALFT
                     ELDSLPEVALTKSLSEASPWGAAPFLPTRNLPAPLPKFLTLTVILQAAFALTAIIIAI
                     PFVANLLPAFEIPSLAESLAQLSTFNLQPATFQLPNFQPANLPTFNFQPATFEISSLL
                     MALAGVSVLWILGNGVFLRNQR"
     gene            4755..5246
                     /locus_tag="PL000_08875"
     CDS             4755..5246
                     /locus_tag="PL000_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89053.1"
                     /translation="MNEFLQIMVVILLFTIPLAAYFLVLAALFPNRAAKTQRVLTTMP
                     GRSFGVGLVNFLFFGLIALVLFSVAENAGSVVKTILMIPALVILAALTLALSFGLTGM
                     VNQLGERIFPEHLAWKRTALGTVILGLACLLPAIGWFLLFPYVGFVGIGAFILGLFQR
                     ETS"
     gene            5434..5952
                     /locus_tag="PL000_08880"
     CDS             5434..5952
                     /locus_tag="PL000_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89054.1"
                     /translation="MADISAIFFVLLILGIAFPAMLTAWWLLFPAIISRAQIRVDKTP
                     WGTFWMGLVILIAVSIPVAILLALPFGPAKFIGWLLLAAALAFSSIGSAGIAAHLGAR
                     LASHSGHSPLNGFIRGAVILELTAFFPVLGWFFIWPLALIMAFGATGFAFLNWMPREK
                     TIHAPEPIPSHA"
     gene            6011..7492
                     /locus_tag="PL000_08885"
     CDS             6011..7492
                     /locus_tag="PL000_08885"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HNC89055.1"
                     /translation="MTLSRRDFLKLASLVSASAALSSCAPVYRRITGDLPALPWTALG
                     AGDFLALNRLTFGPRVEERARFSEIGLQNYIEEQLDFESINDFSCDLQISTFKTLKME
                     ANEIEGVSNELFDGYDRERAPRELREATLLRQLYSKRQLYEVMTEFWSDHFNIFMDKE
                     WEFVLKPVDDREVIRKHALGSFRDLVWASAHSPAMLVYLDNQANEQSHPNENYARELM
                     ELHTLGVNGGYTQKDVMELARCLTGWKVKDHFWLGEFTFDKNIHDTGTKHVMGLSIQP
                     AGVEEAEQVIEMLVKHPSTARFVSTKLARRFLADEPPQEIIEKAVQTFLKTNGDIKSV
                     LRVILLDGLPLAQPKYKRPANFVLSAMRMLNVETDAAALHDYLVRMGQVAFNWPTPDG
                     YPDRSELWQGNLMPRWQFAFELIRNEIKNTKHNLQNLLDAASTGILQDDVDAMTSLLL
                     GAPLERITRDGLIDTVRAAGSTEEETLQIITASIIASPAFQWR"
     gene            7546..8727
                     /locus_tag="PL000_08890"
     CDS             7546..8727
                     /locus_tag="PL000_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HNC89056.1"
                     /translation="MLTQTWLPRFSFAPVNTAPRGDTLVVVFLRGAADVLNMVVPHGE
                     EAYYQLRPSLGIARPDDSKRKKEERVLDLDGFFGFHPNLGPLLEAWQSEQLAIIHACG
                     APDESRSHFKAMELMERGVDDERGPASGWIGRHLATLNTGNTSPLRAVGMGTRPQRSL
                     SGTVPVSALRSIADFHLGGDKHALQQMRASLETVYANDVLGLETLSIMDTLEKLDPST
                     YHSPLSTYPDTEFGLALKQTAMLIKAEVGLEVSAIDLGGWDTHFAQGSSTGLMPNLMK
                     DLAQGLAAFHADMADHQNKLTTVTMSEFGRRASENGSLGTDHGHGSMMMVLGGNVDGG
                     KVHGQWPGLEEGQLIGPGDLAVTTDYRDVLSEVLTKRLNNPATNEIFPDYQPKMPNVM
                     K"
     gene            complement(8777..9814)
                     /locus_tag="PL000_08895"
     CDS             complement(8777..9814)
                     /locus_tag="PL000_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC89057.1"
                     /translation="MWKKIFLCSVLILASCVSISVDSNAQPTAQPDFVTSTLMPTQAG
                     YVPPTLTPVPEATITPTRIPVTAPANCTDSAVLVRDVTIPDNTQVKAGEKFTKTWEFI
                     NNGTCPWIGYTLKFSSGDKMNAPLSAPIPDTLAKERVEASVELTAPASNGTFTGYFTL
                     NDANGNALPIGTEKTFWVKIIVGSGAIVPTTSGGNPTQAVGITPVSNGGGANCSFSQN
                     AGYVNQIASLINAERANAGLPQLTINAELSSAAQGHAADMACNSSISHTGSDGSSAYG
                     RVLASGYSGQFYEEIIYGGGGPEAAMSWWMSDKIHRDAILNPNTTEMGVGYAYFSNGA
                     YGDYITVDFGG"
     gene            complement(9831..12062)
                     /locus_tag="PL000_08900"
     CDS             complement(9831..12062)
                     /locus_tag="PL000_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89058.1"
                     /translation="MDFQPYIPGMKPADPGPLSRFLPPLEEGVISGWLSTQPFGAAQS
                     GPGTWLLDPFGFSPRLVLEAARSGTRVLVTANNPITRFLLEMFANPPPESEFIAALAD
                     LGAVKKGDERLAGHLQSLYLTKCEKCNQQIHANAFLWRKGEDAPYARIYECKHCGDSG
                     EHPATEEDIERAKKIAETDTLHRTRAFEKVVALNDEDRFYAEEAIQHYLPRPLYVITT
                     IVNRLDSLNLSAERKRALTALVLIACDAGNTIWAHPAERPRPKQLSTPSQFREHNVWM
                     VLERGLTLWSETGSTVPCEAWPKKIPESGGICIYEGRLKDLAQLVKKEIPISAVIGSV
                     PRPNQAFWTLSALWAGWLWGKEAVEPYRVALRRRRYDWAWNATALHSAFNHLNDLLPD
                     GVPFFAFLPEPEPSFLTSAFTAASASGFSLVNVALRTEHDPVQVIWKSGKKEKPQTAD
                     DSLLQTSIQEYLASRGEPASYLHVHTAGLIALAEANALKQPEHEFDEALRKTNALFES
                     ALKTDERFVHYSTGENVETGTWSLHFGGGAQAKSESLSDQVEVAIVTFLQKNQHAIYL
                     EVEDELYPRFTGLLTPSKGLIYAVLNSYAEKESGGWKLRAEDLASARRSELNDIFALI
                     EAIGRRLEYKTNRTDKTLTWLENGKPIRSFYVMASALFGRALETTAPDTILVIPGGRA
                     ALAAYKLQRDPALAAQLKTRRVVKYRLLRAILEVPILTRDSFEEQIASDPVEKSAGQM
                     MMF"
     gene            complement(12082..13047)
                     /locus_tag="PL000_08905"
     CDS             complement(12082..13047)
                     /locus_tag="PL000_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC89059.1"
                     /translation="MDFSTNGIVTVIPAYRVQDTITTVLKDLPAYIKHIIVVDDASPD
                     SSVKLVTAAAQQDPRIILIRHERNQGVGGAMISGFHKALELGAKIVVKLDGDGQMDSA
                     HIPALVTPLILGKADFTKGNRFRDFVSLRNMPMVRRVGNLGLSFLTKAATGYWNIFDP
                     TNGFFAIRTEVLKQLPLEAIDKRYFFETSLLANLYLVNAFVMDVPMPARYGNETSHLS
                     IRRSLLEFPLKLFSTLIRRILLKYYIYDFSIISLYIVTSIPLLLFGFIFGAIKWIDYA
                     SRNIPAPTGTVMLPTLCVILGIQIMLSAIEIDMKSTPSQPICDPL"
     gene            13097..14248
                     /locus_tag="PL000_08910"
     CDS             13097..14248
                     /locus_tag="PL000_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89060.1"
                     /translation="MATGERILLVESDPDIVDLIVRQALQPLGYRVGTAGDAASAIKL
                     SMQTPPDLILANLNLPGLSGKDMLAALSAQGVKSPVIVIAEKGQETDAIQAFRLGAAD
                     VIFWPVRDAEVVSIVERALRQTQQSRERQKLDRQLSAMNTELQRRLRDLTTLLSTAKA
                     VISITDQRLLFDRILESAMQVAEADYCWLTLREEKGNAYLLRAHRNLPEAWAKKMNQP
                     LDDGISSLVGFSGESLVMNGSPLQKFKIAALGKSVGVIPIKIQGEVIGLLMVARKIDR
                     EIARDSQTMLEAMADFASISLVNARLFRALEQSAETARMNEKQRHAALDLVRTNIRNE
                     IQSAGYPLDLVLTETPGPLTPEQRKALEQVQAALQRLASSSEKTTIPNK"
     gene            14261..15709
                     /locus_tag="PL000_08915"
     CDS             14261..15709
                     /locus_tag="PL000_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89061.1"
                     /translation="MSKQELILLAMDASPVLGLMERTLRSAGFDVAIAHDLVGLNKCL
                     HEAIPSLMIVGEKFVDQGGFAIAKEILERFPTLPIIFYAEQDTTGYAKTALKMGLSGY
                     LYPPLKVSDITDEVHRSLERSSKLGDWLRREVRRTTSSLTERAKLSESERSKLDAIIA
                     NIQDGVIVLDDRKFILLANHAIHDIFNFHDREIVGRPLADAIKNADLTALLARAGDSP
                     LKYHEINFDDGRVFNAQYTPIPKIGGAITMQDISYLKELDRLKSDFIHTVSHDLRSPL
                     TSVLGYTELIERTGPLNANQLEFLHRLQGSIQHITTLINDLLDLGRLEAGFDTRREVV
                     QLSGVLHYSLDMFDGQLKKKNITLVTDVAPDLHPLRANPIRIRQMLDNLIGNAIKYTP
                     DGGRVTINMFMQDQQVVLKVEDTGYGIPPEEQSRVFEKFYRATNAIEDIEGSGLGLAI
                     VKSIVDSHQGRVWVESVVGKGSAFIVLLPAHE"
     gene            complement(15899..16315)
                     /locus_tag="PL000_08920"
     CDS             complement(15899..16315)
                     /locus_tag="PL000_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNC89062.1"
                     /translation="MNIYFACSITGGREFETAYQKIVAALEKDGHEIPTSHLVQSEIA
                     EKERQLTPQDVYERDVNWIRNCDTLIAEVSTPSHGVGYEIAFALGLGKPVLCIYQKDR
                     RVSKMLSGNPDPKITTQAYGTVEEAISQARFFLDSL"
     gene            complement(16312..16932)
                     /locus_tag="PL000_08925"
     CDS             complement(16312..16932)
                     /locus_tag="PL000_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89063.1"
                     /translation="MAKEKGGRRYPLVIYTHMIKRWWTAVFTLGLALLGLAWAVYSWG
                     MDQWRWLALASIGGLHVFIGLGMWLMRKSAYVQPFREYLKLVTPFLRLNISYRRVRRA
                     TSANMGTLFPPNSVSSWRAEIIQPLAKMTAIVIEMNSHPMPQSQLRLFLSPLFFKDKS
                     PHIVILVDDWMKFSSEMDSLRTGTGPISAQQKRNASILSRLPNKDK"
     gene            complement(16952..17599)
                     /locus_tag="PL000_08930"
     CDS             complement(16952..17599)
                     /locus_tag="PL000_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HNC89064.1"
                     /translation="MHKLEQITDGIRKTFDARTSARDHALAQARQLTRACSLAIRAVH
                     RDEADVMNAQLQEARQLADTLRASLAAYPDLFYAGYTQDALKEFVEANVTCALIRNEP
                     LQTPEELVVEPATFLNGMAEVVGELRRRILDILRHGYSPEAERLLSYMDEIYSVLVTM
                     DYPDAITNGLRRQTDLARSIIEKTRGDITFSLRGEHLTQAISRLSAQLNHDQPSD"
     gene            complement(17602..17940)
                     /locus_tag="PL000_08935"
     CDS             complement(17602..17940)
                     /locus_tag="PL000_08935"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNC89065.1"
                     /translation="MTTPPIPSKPTGPILELPADLELVYANLARIAHSPADIVLDFAH
                     LLPGEPRAKIKSRVVMTPLSAKLLVRALTENIARYESAFGEINIPSNNSLADNLFRPF
                     QQPPEPPKEP"
     gene            complement(18003..18434)
                     /gene="ssb"
                     /locus_tag="PL000_08940"
     CDS             complement(18003..18434)
                     /gene="ssb"
                     /locus_tag="PL000_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC89066.1"
                     /translation="MYHTIIIVGNVGKDPEMRYTPSGQAVTSFSVATSRQYTAGNGEQ
                     VKETIWFRVSTWGKTAEVCNQYVKKGSKVLVEGRLTPDKNTGGPRIWSKQDGTAGASF
                     EVTASTVRFLSSRGEAHESGPIAGGGGMEMAELPPEDDIPF"
     gene            18587..19120
                     /locus_tag="PL000_08945"
     CDS             18587..19120
                     /locus_tag="PL000_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNC89067.1"
                     /translation="MSETTPKRYHPLHVAIHWLMALLVFLMLGVGKVVMPGISPEDPQ
                     KVMMLQSHTYIGGAIAVLFVIRLILLFTTRRPAPADAGNAFLNFVGKAVHLLLYLFLA
                     GMAVSGAGLFQMAGLPDVFSAAKPYPSNFFDYLPRMGHGLLSWLLLALIALHFGAAMY
                     HQFFKKDNLLARMWFGK"
     gene            19176..19541
                     /locus_tag="PL000_08950"
     CDS             19176..19541
                     /locus_tag="PL000_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89068.1"
                     /translation="MNWQITYIADENLIYVKTSGVVTSEAANSMLKEIVEVAVCHQCD
                     RQIIDHSGADFALRLFELYERPSINVRIGLSHKWKIAMIFSELTQNTFFMETVFRNRG
                     YNFRQFDNLEKARTWILSE"
     gene            complement(19780..20334)
                     /locus_tag="PL000_08955"
     CDS             complement(19780..20334)
                     /locus_tag="PL000_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89069.1"
                     /translation="MITSEVLNPLILSAFTKEITWLEAASRFSLDELIETFYYTRERT
                     EENLAPLTDAQVAYTSQAHSLWSLSESITHLIYTQGFYHNKLLEISTSQLPHMVEAAR
                     GFGEGARQNVSANDLRVSLATATERIHATIEQTRHAHDPQKTEISSAFGICNYQTWIL
                     LMLAHEVDHLRQMVAMRRLARTST"
     gene            complement(20365..21552)
                     /locus_tag="PL000_08960"
     CDS             complement(20365..21552)
                     /locus_tag="PL000_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC89070.1"
                     /translation="MNKTFVKPRYDSGGFAGTPNRIIEAFASKKYDAVVLFFIDAFGW
                     RFYERFQDAPFIKRIERTGKIEKLTSQFPSTTAAHVTTMHTGLPVGESGVFEWQYYEP
                     QVDHIITPLLFSFAGTRDRDNLKNKKVNPASIYPKGILYPELAKMGVESFNFGLRDYT
                     PSTYSSVVMEGSEPHAFKTLSEALVNVGLLLEKQQNPTYIHLYFDKIDALAHEYGPNA
                     PQTEAEIQTFLLMMEHYFETIFNGKKRILFLMTADHGMCEIDPKTTIFLNTHPEFDGI
                     QRLIRKNRKDRLIVPAGSARDMFLYIKDGFLDEAQDFLARRLEGRADVVKTEALIADG
                     YFGKEISPRFLERVGNLVILSYRYESVWWYEKDKFEQNFYGHHGGLTPQEMETILYSC
                     ETG"
     gene            complement(21593..22078)
                     /locus_tag="PL000_08965"
     CDS             complement(21593..22078)
                     /locus_tag="PL000_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC89071.1"
                     /translation="MPTISRPKLPKGYVDHPIAEVPWEHVEKRLTESINYWLCTVYPD
                     GRPHVVPRWGAFLDGKLYYDGSPETRHARNLEKNPNTTLHLESGNDVVIMEGTSTPAS
                     KPDLELAQRLSAVYCAKYASDGYAPKPDQWDEGGLYIFTPRQCLAWTKFFENPTKFIF
                     E"
     gene            complement(22081..22989)
                     /locus_tag="PL000_08970"
     CDS             complement(22081..22989)
                     /locus_tag="PL000_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC89072.1"
                     /translation="MNRITKIVLGVILGLLVVVAAGLLLKTRSEAYNLLHSPMETRKL
                     PIDTPDDLKLEFESVTVTNPDDMELAGWFIPGENDAVIIMQHGYKSTRSELLNEAEMM
                     HRHGYNVLVTSIRAHDESEGELITLGAYEVQDMEAWYQYLLTRTEIDPEKIGILGNSY
                     GGMLAIQFASQNENIKAVVANSAFSSMQDTVATSVKHFTGLSEFPFVPLIVFWAEQQT
                     GIKIEEINATEWIPLISPRPVFLMQGGRDTVISPVSGERLYNAAGEPKELWFDEKLGH
                     VEFDTRRTFEYENRVAAFFDRYLLGE"
     gene            complement(23023..25356)
                     /locus_tag="PL000_08975"
     CDS             complement(23023..25356)
                     /locus_tag="PL000_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC89073.1"
                     /translation="MTEETPPVEQTPEPQNRGTGALILFLFFALPMPFCLFIYHFVLW
                     STEQSAIISSSTKDFAWAGPLGLLGQAILMSILTALLWYFTKDDRFKPVYAGLLGASL
                     MGFPALALRAIGPNNDQLGFIAQMLLCGIGTGVVILIRKNKLKWDSASLPFGLVVAGI
                     GALPFAIYGSLGLAGDVVLSLFASAAFGLLAAVLIESSTENVFLDGIGIGAVLALLGS
                     SLGYDGAQLLLLAALPSFAFAVAAVMPSRAAAGAAVSMLTFVTLAMFDPTELTFVLGD
                     IGGLAMSAGGWVMVIGWGVSIVALILRFVTATSSRSVTKRAIGWAGAGVAWILVVAVF
                     FIFGNPGSYGDRLFVILKDQADLSDVAKIKDRDERLTEAYTRLTAHANETQSELRAFF
                     DKAGIEYTPYYLENAMEVRGGTLVRLYLMTRPEVDRVIPSPRLRAAPEDAAASEGAMD
                     VESNAQWNIAMIGADQVWNEFGVTGEGIVVGQSDSGVDGNHPAIREQYRGFNSGDDYN
                     WFDPWDGTTSPNDEGGHGTHTTGTILGVNGIGVAPGAQWIGCVNLDRNLANPALYLDC
                     MQFMLAPFPHGGDPLKDGDPTKAAHVLNNSWGCPSIEGCDPNALLYAANNLRDAGIFV
                     VVSTGNDGPNCETVESPLSLYDSVFSVGAIDILGNMADFSSRGPVTADGSGRTKPDIV
                     APGVNILSSIPGGYYAEFSGTSMAGPHIVGVVALIWSAQPELIGDIDATEQLIIDTAK
                     PYTGNISSGCFTGSNPSNAFGYGVVDVYAAVKQALGK"
     gene            complement(25498..26151)
                     /locus_tag="PL000_08980"
     CDS             complement(25498..26151)
                     /locus_tag="PL000_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89074.1"
                     /translation="MKNILKPVRWNTFFRDFIRIEIGFMLFGLAIVLMIQGNLGTSAW
                     AVLEVALAHKIGISIGTMTVMMGFIVLSGALIMREKIGWGTLGNILSIGPWEDLWLSI
                     IPSVKDNLPVQGGMLLLAILLMGIASAIYIGVDAGAGPRDSMMLAIKRTTGISIRVAR
                     AIIEVTVVTIGWLLGGPVGIGTLVYALLVGPSVQWGFKIFHVQPHGPEALVPQAPED"
     gene            26199..26642
                     /locus_tag="PL000_08985"
     CDS             26199..26642
                     /locus_tag="PL000_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89075.1"
                     /translation="MTQIIEVQREQFTISTDPARLNLETILDFFTRAYWTQGRPPERT
                     KRAIENSLVFGVYEGTKQIGIARVVSDLSIFAYLCDVFIHEDYRAHGLGKWMLETIMA
                     HPDLQGIRRWTLVTRDAHGLYKQYGFDALTNPDGWMEMIRPFPGE"
     gene            26730..27746
                     /locus_tag="PL000_08990"
     CDS             26730..27746
                     /locus_tag="PL000_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC89076.1"
                     /translation="MKIKNWQFWLGVLISVIFIWLSLRGLKLNEFWGTVQKANYLWLI
                     PGIGVYFVGVWVRAWRWHYLLKPIKEIPTKTMFPVTTIGYMGNNIYPARAGEVLRAVI
                     LKRKEGVPVSASLATIIVERIFDGVVMLAFVFVNLSELAKLTGASGFVGNIQQVAIIG
                     TGVFLGALIVFLLAAMFPHTTAKIGLWLIEHLTPKRLHTKIIGIMNKFLDGLASLRSP
                     LNVLMVFLTSVIIWLLETGKYWFVMHAFSFNVSFFALMLMNGIVNLATTIPSAPGYIG
                     TFDAPGIAVLTAYGVDQATAAGYTLTLHVALWLPITILGAYYLAREGIKWSDTLREET
                     ENTD"
     gene            27751..28338
                     /locus_tag="PL000_08995"
     CDS             27751..28338
                     /locus_tag="PL000_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNC89077.1"
                     /translation="MTTRIYVDWSGDPGFKFRQGSSELLVIAAVMADEEPNIGNLREK
                     LSLPDNYEFHFSKADHRIREHFRNYLNTELEIPGVVVLRADKQMFSLNMRQKRGEQVV
                     ADLIAQCVVNLPAELLHDSTLYYDGEKEQESFKNTLRVTLSQTLHSGVFLRSVKAVPA
                     SRNDGLQVADMLAGFVRNNPSAIKSSIVKLIHYPD"
     gene            28474..29748
                     /locus_tag="PL000_09000"
     CDS             28474..29748
                     /locus_tag="PL000_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC89078.1"
                     /translation="MKIAIVGAGYGGMVAAWDLKKAGHDVTIFESAEYAGGLASGFKE
                     PHWDWSVEKFYHHWFQSDGEMLGLIRELGLEDKVRFPRPLTVMYYKGKWYPFDSILNA
                     LLFPGLGWGLNKIRFGFVGLFLRLTNNWRALEKVTADEWMMKYAGRQVYEQMWKPLLV
                     GKFASFYKDVNMAWLWARMHARTTRLGTFEGGFQKFADLFAEKLRAQGVQIKLGVSIK
                     SIKRDQASGSLLVDDQPFDKVLVTSSPSLMAKLCPDLPESYLKGLLGLKSMGAVVMTL
                     SLAHPLSKEGYYWFNVPKEEGYPFLALVEHTNFVPKENFGGDHIVYAGDYLELGHEYF
                     SMSDEQLLERFIPAFQKFNPEFSRDWVKKIWVHKTNYAQPVPLVDHSKNIPEIQTPIE
                     GLYFASMSQVYPWDRGTNFAVEIGRKAARMMK"
     gene            29840..30466
                     /locus_tag="PL000_09005"
     CDS             29840..30466
                     /locus_tag="PL000_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNC89079.1"
                     /translation="MWFYLLQGIGFGFAAASQPGPFQTYLISQTLTRGWKHTLPAALA
                     PLVSDGPIILLCVLVLSQVPAWMQRGLYIAGGLFIIYLAYGTYRTWENFDTAAQTTAP
                     VGEVQQSVLKAAMMNALNPNPYIFWTLVTGPILLSGWRQSPILGVGFLAAFYLTMIAG
                     LAAVILIFGFAQRFGAKFNRVMLGISAIALFCFGIYQLWLGLFPKEIL"
     gene            30463..30768
                     /locus_tag="PL000_09010"
     CDS             30463..30768
                     /locus_tag="PL000_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNC89080.1"
                     /translation="MNIVLVHVHVKPEFVEAFKQASIENASNSINEEGIARFDVIQQN
                     DDPTRFILVEVYKTAEASAAHKETAHYAQWRDTVAEMMAEPRQGIKYANIHPEDSGW"
     gene            30772..>31927
                     /locus_tag="PL000_09015"
     CDS             30772..>31927
                     /locus_tag="PL000_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNC89081.1"
                     /translation="MPAFEFATANRVIFGAGKLSELGNQVKGRAKRLLLVRGNSSDAI
                     PRVKEILSGQDILFNEFALHGEPTVATASEGVDLALQHGVDMVVALGGGSVLDAGKAI
                     SALTTNRGDVFDYLEVIGKGQPLVNAPLPFIAIPTTAGTGTEVTRNAVLGSPAHGVKV
                     SLRSPMMLPSLALVDPALTYTLPPAITASSGLDALTQLIEPFVSVKANPMTDAICREG
                     IRRAARSLRHAWHNGAEVAAREDMSLASLFGGMALANAALGAVHGFAGPLGGMLNAPH
                     GAICAKLLPLVMEANLKALESRHAGHVAIERYTEIARMTTGNESASAWDGVQWVSDLV
                     RELKIPPLSTHGMNAAHIPEAVQKTLNASSFKGNPIPLNEGELREILEKAL"
BASE COUNT         7547 a         7891 c         8483 g         7906 t
ORIGIN      
        1 ccaacaaagc gtgcatctga cgctggggac tgcccgtagg gcgtgcgcac atcccaagca
       61 gttttctacg cctgagcatt tttctggtta gacggcttcg ccgtccccgc cccagcgcag
      121 gtaacgcaaa cccttaggct catcaggtaa ataaaaaaat gacaattgat aatcgtcagt
      181 ttagttcacg cgagcaggaa acaataaacc ttctcttgca gggcaaaagt aataaacaaa
      241 ttgctctgtc acttaatgtt tccataagta cagtcgagtt ccatctaaaa aatatttata
      301 gaaaacttca ggtaaaatcc agaaccgaag cgatcttgcg actagggaaa cccacaggtg
      361 atgatgaatc tagaacacaa gggcaatcca taggcagcaa agttgataaa caaagggaat
      421 ccatagtaga caaaattagt gtggacgccg aaaatggcgg caacactaat tcacatcgga
      481 ggattcctat gaaacaaagt tcttacatcg tcggcggtag tctgttattt ttaattgtct
      541 tggctgcgat agccatattt gtaaaagtgt atggacacac tttatctgcc ccgccaacaa
      601 cccaagcgcc gctaagtact gcaaccatgc aacctgcgat agtatctaca acagcgcctg
      661 cgactcctga atcgataacg gcaacatgtc ctgttgaaac tgtagattta aaacttttca
      721 caaacaccaa tgatggttat tgcttccttt atccaataga aaatactgct tttccgccca
      781 ataaggttgt cattaatcca aacggcatat ctggaggaga ctttcttcca ggggactttt
      841 atgtattaat ccaagtggaa gccgcttcag gtcgcactac tacccaagtg gcaaatgaaa
      901 aaattgcaga ggcgggagga gtcctcccta tcacacttac ctcaatattg gtagacggaa
      961 aagaagcagc tgttatagat ggtcttccta gtcaggactc tacccgtcat gttttcatta
     1021 ttgccaatga ccgtttgtac acattcttct tcacaccttg ggacccacgg attgagggat
     1081 tctctcagtc cttattgaaa ttatattcaa cggtnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnttttta tggcattctt gtttttagcc aagttaatca tgataccgct
     1261 tcgtggtttc aagataaaaa aagaatttga taagcctttg tacccaattg tcatttttac
     1321 gttcttgttt ttgacagcat cattctttga catcattgtt attccaaaac tatgggtgga
     1381 aaaatttgga ttgtcaacag aaggcgttgt agtagatctt cgggctacac atttccgtag
     1441 aagaagttat gtggcgactt atgagtttga taaggatagt ttgcgttttg taaaaaccca
     1501 aagcattagt tcttctatgt atgaacaact ggaaacacat ccaaaagcag agataaggta
     1561 tttgccgata aatccagata tctcttactt gagagatttg gaatatctaa aaatgaacac
     1621 atttgtctct cttgtgtcag ggattggcat tatgattctt ttgtatagtt ataagccagc
     1681 agaagatttc aaccatacca gtgatgtcaa ataacaagct gcccagcaaa gcatggatct
     1741 gacgtgcgtg ggcgttcttt tgcaggtttg gggtctatat ttccttcttg acgaaataga
     1801 acatgggttc tacaatttcg ccaaggagaa tatccatgac aaaaacgatc gtttcgccta
     1861 aaagcgagtc cgcgcaaaat gcggcttcgg ttaaggagtt tattgcttca ctggatgagc
     1921 aaaccctgaa agatagcaag gcgcttattg caatgatgca gggaattagt ggaagcaaac
     1981 ccaggttgtg gaatgtaggt acgattgggt ttgactccta tcattacaaa tacgatagcg
     2041 gacgcgaagg cgacagtttt atcattggct tttatccaag aaaaggcaag ataaccgtct
     2101 acctcatgga tggcatagct cgttactctg acttactggc caggttaggc aagcacacga
     2161 ccactgggta ctgcgtttat atcaagcacc ttggcgatgt ggagttgccg atcctcgaac
     2221 aaatcctgca gagatcgtat gagaatataa agtcacagga tggacatatc aaccagatat
     2281 tgtggaagac tgaaaaatag gcgttttcgc catgttttat catgtgctct gtgagcaaac
     2341 ctaaaaaagc atgtatctgg gatgccgttt gatatataga aatagtgagg ccgtatggag
     2401 ttttcaacaa tttactatga acttcaacaa agtaccgaga tgattcgcgc tttgttgact
     2461 ggcattacac aggaagaggc gcgtgtaaag ccgaacgcgg aggcatggtc gattcttgaa
     2521 gtggtatgcc atttgcacga tgtggagcgc gaagattttc gagaacatct cgattgcatt
     2581 cttcaccgtc aggatgaaaa atggcatgag attgaccccc aaagctggat aacatcacgg
     2641 aaatataacg agcaaaattt tgctgaaatg caagagaagt tttttgctga acgcaaaaag
     2701 tcactcgaat ggctcggcgg actgcagaac ccagattggg agaaggttta cacaacggag
     2761 tatcatgcat ttagcgcagg cgagatgttt tcctgttggg ccgctcatga taatcttcac
     2821 atccgccagt tggtggaact cagacgtttg aggctggaac atgtcacaaa gccatataat
     2881 attgaatacg caggtgattg gtaattgcac agcacaaaag aaggctcaac ttaagtttgt
     2941 tgtttgggtt gtgactgttg gatcgtgctg aaagttcaat gccttaatgg taatgcaaag
     3001 atgaataagg agttttacta tgaataacaa gtctaatgct cgagtgtatg caatgaagtt
     3061 ctcaggtgtg tatccgctct atgttcaaaa ggcggaacgc aaaagccgca caaaagaaga
     3121 agttgacaag atcatttgct ggttgactgg gtatagcgag gcggaattac gtcagcaaat
     3181 tgaacaggga aacgattttg aaaccttttt tgcgcaggcg ccagccttga atccgaaccg
     3241 ctcgcttatt aaaggcgtga tatgcggcgt tcgtgtggag gatatcgaag ataccttgat
     3301 gcaaaatgta cgatatttgg ataagctgat cgatgaactc gcaaaaggga aggcgatgga
     3361 gaagattttg agatagtagg gcggatagca attgataact ccactgcaaa aaggcaatcg
     3421 aaccgcaaag cacgcgaagc ccgccaagga aaagaaagat ctcaggcgct ttctcaggaa
     3481 aattccttct gaagatttaa gttgaaccgt tttgaatttt cttcgcgctc tttgcggcct
     3541 tcgcggttgg aaaaggtttt gacagcgatt caccccccat tctgcaactt tttcccgttg
     3601 actcctgtat taaactctag ccatgaccac gccaactgac cgcgacctga tcctgcaaac
     3661 ccgtcgcggt gacgctgaca gtcggacggc atttggcgag cttgtcacac gctaccagac
     3721 gagtgtcttc aacgtctgtt accgtatttt gcacgagcgc ggcgaagccg aagaccttgc
     3781 acaggagacc tttatccgcg cctacgaccg catccatacc tttgatatcg aacgcgaatt
     3841 cgggccgtgg attcggcgtg tggcggccaa cttgtgcctg aataccattg agtcgcagaa
     3901 ggttacgtct gagctcgacg atgaacggga cgctgattct gcccaattgc ctgaggcagt
     3961 tcaagaggtc cgcgagagaa gcgaacaaat tcgccacgcc ctcgcatccc tgcccgtgca
     4021 ttatcgtgcc gtcgtcgaat tgcgtcattt tcaagaacta tcctacgaag aaattgccgc
     4081 agaattgaac atcccattga gcgacgtgaa gagccatctc ttccgcgccc gcaaaatttt
     4141 ggcggagaaa ctccatgcat ctcactgacg agcaactcaa cgaatatctc gacaacgaaa
     4201 ccaacgagcg tatgcagatc gaaacgcatc tatcttcatg cggcgaatgc gcggcgcgac
     4261 tttctgcgct tcaggcattg ttcaccgaat tggattccct gcccgaagtg gcattgacca
     4321 aatcccttag cgaagcatcc ccgtggggcg ccgcgccgtt cctgccgacc cgaaacctgc
     4381 cagccccact gccaaaattc ctcacgctga cagtgatcct gcaggccgct ttcgcgttga
     4441 ccgccatcat cattgccata ccgttcgttg caaacctgtt gcccgccttt gaaatacctt
     4501 ctctggcaga aagccttgcc caactttcaa ccttcaacct gcaacctgca accttccaac
     4561 ttcccaactt tcaacccgcg aacctgccca ccttcaattt tcaacctgca accttcgaaa
     4621 tatccagcct gttgatggcg cttgcgggcg tttccgtgct gtggatcctg ggcaacggtg
     4681 tatttttgag aaaccaaagg tagggatacg gcgctgctgt atccgtgaaa atagtaattc
     4741 aacaaggaga attcatgaac gagttcctgc aaattatggt tgtcatcctg ctgtttacga
     4801 ttcctctcgc tgcgtatttt cttgtcctcg ctgcgttgtt tccgaaccgt gccgccaaaa
     4861 cccaacgcgt acttacgacc atgcctgggc gttccttcgg ggtggggctg gtcaactttt
     4921 tgttcttcgg gctgatcgca ctggtgcttt tttccgttgc tgaaaatgca ggcagtgttg
     4981 tgaaaaccat tcttatgatc cctgccttgg tgatccttgc cgcgcttaca cttgccctga
     5041 gcttcggcct gacgggcatg gtcaaccaac ttggcgagcg catcttcccc gagcatctcg
     5101 catggaaacg aactgcattg ggaacggtga tcctgggtct tgcctgtctc ctgcctgcca
     5161 tcggttggtt cctgctgttt ccgtatgtgg gttttgtcgg cattggtgca tttattttgg
     5221 gattattcca gcgcgaaaca agctaatcag gtaaaagaaa ttttcacacc gccgagagac
     5281 gcgggcacgc agagaaaatt ctgcgcaccc gcgtctttgc tgttaatcca ctcgcaactt
     5341 accattcacc attcactatt tatttcccct gcaacttttc cccacccatc cctgtacaac
     5401 ctataactct caaaacctga aaggaacttt cccatggccg acatatccgc catcttcttc
     5461 gtcctgttga ttctaggtat tgccttccca gccatgctca ccgcgtggtg gctgttgttc
     5521 cctgcgatca tttcccgcgc ccaaatccgc gtggataaaa ccccctgggg cacattctgg
     5581 atgggacttg ttattttgat cgcggtcagc attcctgttg ccattttgct tgcgcttcct
     5641 tttggccctg ccaaattcat cgggtggttg ttgcttgcgg cggcattggc attttcctcc
     5701 atcggctcgg cagggattgc ggctcatttg ggcgcacgcc tcgccagcca cagcggacac
     5761 tctccgctga atggtttcat ccgcggcgca gtcattctcg aactcaccgc cttctttcct
     5821 gtgctgggtt ggttcttcat ctggccgctt gctctcatca tggcttttgg cgccacaggg
     5881 tttgcctttt tgaattggat gccgcgcgaa aaaaccatcc acgcacctga gcccatccca
     5941 tcgcacgcat aaaccaaccg cgaaaaatct ttcctcccat ccactttcca ctcactgata
     6001 actgatcacc atgacccttt cccgtagaga cttcctcaaa cttgcctccc ttgtttctgc
     6061 cagcgcggcg ctttcctctt gcgcacccgt gtaccgcaga attacaggtg acctccccgc
     6121 cctgccgtgg acggcgcttg gcgcaggtga tttccttgct ttgaaccgtc tcacgttcgg
     6181 tccgcgtgtg gaggagcgcg cgcgtttcag tgagatcggt ttgcagaatt acatcgaaga
     6241 acaactcgat tttgaatcca tcaatgactt ttcctgcgat ctccagattt caaccttcaa
     6301 aaccctgaaa atggaagcca acgagatcga aggggtcagc aatgaactct tcgatggcta
     6361 tgacagagaa cgcgctccac gggaactgcg tgaagccacc ctgcttcgtc aactgtacag
     6421 caagcgccag ttgtacgaag tgatgaccga attctggagc gaccatttca acatattcat
     6481 ggataaggaa tgggagttcg tcctcaaacc tgtggatgac cgcgaagtga ttcgcaaaca
     6541 tgcgctcggc agtttccgcg acctggtttg ggcatcagcg cactcgccag ccatgctggt
     6601 ctatctcgat aatcaggcca atgagcagag ccatcccaac gaaaattatg cacgagagtt
     6661 gatggagctt cacaccctcg gtgtgaacgg cggctacact caaaaagatg tgatggaact
     6721 tgcgcgttgc cttacaggct ggaaggtgaa ggatcatttc tggctgggtg aattcacctt
     6781 tgacaagaac atccatgata cggggacgaa acatgtcatg ggtctttcga tccagcctgc
     6841 gggtgtggaa gaagcggagc aggtcatcga aatgctggtg aagcatccga gcacggcgcg
     6901 cttcgtttcc accaaactgg cccgccgctt cctcgccgat gagccgccgc aggagatcat
     6961 cgaaaaagcc gtgcaaacgt tcctcaaaac gaacggcgat atcaaatcgg ttctgcgagt
     7021 cattttgctc gatggcctgc cgcttgcaca gccaaaatac aaacgccccg caaattttgt
     7081 gttgtcggcc atgcgcatgt tgaatgtcga aaccgatgcg gctgcgctcc acgattacct
     7141 cgtccgcatg gggcaggttg cgttcaactg gcccacaccc gatggctacc ccgaccgcag
     7201 cgaactgtgg cagggcaacc tcatgccgcg ctggcaattc gcttttgaat tgatccgcaa
     7261 cgagatcaaa aacacaaaac ataatttaca aaatttgttg gatgcggcat ctacgggcat
     7321 tttacaagac gatgtggatg cgatgacatc cctgttattg ggcgccccgc tggagcgcat
     7381 cacccgcgac gggttgattg ataccgtccg agcggcaggc tccaccgaag aagagaccct
     7441 gcagatcatc accgccagta tcatcgcctc tcctgcattt cagtggcgct gattcgtacc
     7501 gcaacattca tgttgcgata cggtactttt accaaggata ctttaatgct tactcaaacc
     7561 tggcttcccc gtttttcttt tgcccccgtc aacaccgccc cgcgcggaga taccctcgtc
     7621 gtcgtttttc tccgcggtgc agccgatgtg ttaaacatgg tcgtgccgca tggcgaagag
     7681 gcgtattatc aactgcggcc ttcacttgga attgcaagac ccgatgactc caaaaggaag
     7741 aaggaggaaa gggttttgga tctggacgga ttttttggtt ttcatccaaa tctgggtcct
     7801 ttgcttgaag cctggcaaag tgaacagctt gccatcatcc atgcctgcgg cgcacccgat
     7861 gaatcgcgca gccacttcaa ggcgatggaa ctcatggagc gcggcgtgga cgatgaacgc
     7921 ggccccgctt caggttggat cgggcgtcat ctggctacgt tgaacactgg caacacatct
     7981 ccgctgcgag cggtgggaat gggcacccgt ccacagcgca gcttgagcgg aacggttccc
     8041 gtctctgcct tgcgctccat tgctgatttc catctcggcg gcgacaagca tgcccttcaa
     8101 caaatgcgcg cttcccttga aaccgtctac gccaatgacg ttttggggct ggagacttta
     8161 tctattatgg atacgttgga aaaactggat ccatccactt accactcgcc gctttccacc
     8221 taccccgata cagaattcgg cctcgcgctc aaacaaaccg ccatgctcat caaagcggaa
     8281 gtgggactcg aagtctcagc cattgacctt ggcggctggg atacccattt cgcgcaaggc
     8341 tcatccactg gcctgatgcc caacctgatg aaagacctcg cgcaaggtct cgccgccttc
     8401 catgccgata tggctgacca tcaaaataaa cttaccactg tcaccatgtc tgagtttgga
     8461 cggcgtgctt ctgaaaatgg cagccttggc acagaccacg gtcacggcag catgatgatg
     8521 gttttaggcg gcaacgtgga tggaggcaag gtccacgggc agtggcctgg gttggaggaa
     8581 ggtcaactga tcggccctgg tgatctggca gtcaccaccg attatcgcga cgtgctttct
     8641 gaagttttga ccaaacgttt gaataaccct gcgacaaatg agattttccc cgactaccaa
     8701 ccgaagatgc cgaatgtgat gaaataaaaa gtagggacac agcgccgctg tgtccctaca
     8761 gtgtccctac gacattttat ccaccaaaat ccacagtgat gtaatctccg taagcgccgt
     8821 ttgaaaaata agcgtagcca acacccatct cggtggtgtt ggggttgagg atcgcatcgc
     8881 gatggatctt gtcactcatc caccaactca tcgcggcttc gggtccgccg ccgccgtaga
     8941 tgatctcttc atagaattgg cctgaatacc ccgaagccag aacccgcccg tatgccgatg
     9001 agccgtctga gcctgtgtgg ctgatgctgc tgttgcaggc catgtcggcg gcgtgtccct
     9061 gcgcggcgga agatagctcg gcattgatcg tcaattgtgg caggccagca ttggcccgct
     9121 ccgcgttgat gagcgaagcg atctgattca cgtagcccgc gttctgactg aaactgcaat
     9181 tcgcgccgcc gccgttgctt acgggtgtga ttccaaccgc ctgggttggg ttgcctcccg
     9241 atgtggttgg gacaatggcc ccgctcccca cgatgatctt cacccaaaat gttttttcgg
     9301 tgccgatggg cagggcattt ccgttcgcgt cgttcagggt gaaataccct gtgaaggtgc
     9361 cgttggaggc tggggcggtc aactccaccg aggcttcgac tctttccttc gcaagcgtat
     9421 caggaatcgg cgcggagaga ggcgcgttca ttttgtcgcc cgatgaaaac ttgagcgtgt
     9481 atccgatcca cgggcaggtt ccgttgttga tgaattccca ggtcttggtg aatttctcgc
     9541 ctgctttcac ctgcgtgtta tcagggatgg ttacatcgcg cacgagcaca gcgctgtcgg
     9601 tgcaattggc aggcgcggtg acgggaatgc gtgtgggtgt gatggtggct tcgggcacag
     9661 gtgtgagcgt gggcggcacg taccctgcct gggtgggcat caatgttgag gttacgaaat
     9721 caggctgtgc ggtgggttgt gcgttcgaat ccacggagat ggaaacgcag gaagccaaaa
     9781 tcaacacact gcaaagaaaa attttcttcc acataaaaac tccacttgct tcaaaacatc
     9841 atcatctgtc ccgcagattt ttccacaggg tcactggcga tctgttcttc aaaagagtca
     9901 cgcgtcagaa tcgggacctc aagaatggcg cgcaacaaac gatatttcac cacgcggcgg
     9961 gttttcaatt gcgcggcgag ggcggggtcg cgttgaagtt tgtaggcggc gagtgcggcg
    10021 cgcccaccgg ggatgacgag aatggtatct ggtgcggtcg tttcaagcgc acgcccgaac
    10081 agcgcggagg ccatcacata aaacgagcgg atgggttttc cattttcaag ccaggtcagg
    10141 gttttgtctg tgcggttggt tttgtattcc aaccgcctgc cgatggcttc gatcagggca
    10201 aagatgtcgt tcaattcact gcgccgtgcc gatgcaaggt cttccgcgcg cagtttccat
    10261 ccgcctgatt ctttttctgc atatgaattc agcacagcgt agatcaaccc tttggagggc
    10321 gtgagcaatc ccgtaaaacg cggatacagt tcatcttcca cttcaaggta gatggcatgt
    10381 tgattctttt gcaggaaggt gacgatggcc acctccacct ggtctgaaag cgattcgctt
    10441 tttgcctgtg ccccgccgcc gaaatgaagg ctccacgttc cagtttccac attttcgcct
    10501 gtcgaataat ggacgaagcg ttcatcggtt ttcaatgctg attcaaaaag cgcattcgtt
    10561 tttcgcaggg cttcatcgaa ttcatgctcg ggttgtttga gcgcgttcgc ttcggccagc
    10621 gcgattaacc ccgccgtgtg gacgtgtaaa taactggcag gctctccacg tgatgcgaga
    10681 tattcctgaa tcgaagtttg caaaagagag tcatcagccg tttgcggctt ttcttttttg
    10741 ccgcttttcc agatcacctg cacggggtca tgctcggtgc gcaaagccac attcaccagc
    10801 gaaaaccccg aagcacttgc cgcggtaaat gcagaagtga ggaacgatgg ttcgggctca
    10861 ggcagaaaag caaaaaatgg aacgccatcg ggcagcaggt cattcaaatg gttgaaggct
    10921 gaatgcagcg cagttgcatt ccacgcccaa tcatagcggc ggcggcgcaa ggccaccctg
    10981 taaggttcca cggcttcctt gccccacagc cagcccgccc acaaggcaga gagagtccaa
    11041 aatgcctgat tggggcgcgg aaccgaaccg atgaccgcag aaattgggat ctcttttttg
    11101 acgagttgtg cgaggtcttt gaggcggcct tcgtagatgc agatgccgcc gctttctgga
    11161 attttcttcg gccacgcttc acacgggacc gttgaacctg tttctgacca aagagtgagt
    11221 ccacgctcaa gcaccatcca cacattgtgc tcgcggaatt gactcggcgt gctcaactgc
    11281 ttcggccgtg gacgttccgc agggtgtgcc cagatggtat tgcctgcatc acacgcaatg
    11341 aggacgagag cagtcaacgc ccgctttcgc tctgcggata gatttaaact atccaggcga
    11401 ttgacgatgg tggtgatcac atacagcgga cggggtaaat aatgctgaat ggcctcttct
    11461 gcgtagaaac ggtcttcatc gtttaaggcc acaacttttt cgaaggcgcg tgtgcggtgc
    11521 aaggtgtcgg tttcggcgat cttttttgcg cgttcgatat cttcttcggt ggcggggtgc
    11581 tcacccgagt cgccgcagtg tttgcactca tatatgcggg cgtagggggc atcttcacct
    11641 ttgcgccaga gaaaagcatt ggcatggatc tgttgattgc atttttcgca tttggtgaga
    11701 tacagagatt gcaaatggcc cgcgagtctt tcgtcccctt ttttgacggc gccgaggtct
    11761 gccagtgcgg cgatgaattc tgattctggc ggcgggttgg cgaacatttc cagcagaaaa
    11821 cgcgtgatcg ggttgtttgc tgtcaccagc acacgggttc cgctgcgggc agcttcaagg
    11881 acgagtcgcg gggagaatcc gaatgggtcg agcagccagg taccgggtcc gctttgagcc
    11941 gcaccgaagg gctgagtcga gagccagccg gagatgactc cctcttcgag cgggggcaga
    12001 aagcgcgaga gcggtcccgg gtcggcgggt ttcatccctg gaatgtaagg ttgaaaatcc
    12061 atcttgggtt aagtatacaa cctataaagg atcgcaaatt ggctggctgg gtgtggactt
    12121 catgtcaatt tcgatggcag agagcatgat ctgaatgccg aggatgacgc ataaagtggg
    12181 gagcatcaca gtgcctgtgg gggcggggat gttgcggctg gcgtagtcga tccatttgat
    12241 ggcgccgaag atgaaaccaa acagcagcag cggaatgctg gtgacgatgt acagggaaat
    12301 gatggagaaa tcgtagatgt agtactttaa cagaatgcgc cgaatcagcg tggaaaaaag
    12361 tttaagcgga aactccaaaa ggctgcggcg gatggaaagg tgtgaggttt cgttcccgta
    12421 tcgggcgggc atgggcacat ccatcacaaa tgcgttgacg aggtacagat tcgccagcag
    12481 tgaggtttcg aagaaataac gtttatcaat ggcttcgagg ggtagttgtt tcagcacttc
    12541 ggtgcggatg gcgaaaaagc cgttggtggg gtcaaagata ttccagtacc ccgtggcggc
    12601 tttggtgaga aaactcagcc ccaggttacc cacacgccgt accatgggca tattgcgtag
    12661 tgaaacgaaa tcacgaaaac ggttgccttt ggtgaaatcg gctttgccga ggatcaacgg
    12721 ggtaacgagg gcggggatgt gagcggaatc catttgccca tcgccatcga gtttgacgac
    12781 gatctttgcg ccgagttcca gcgctttgtg aaagcccgag atcatggctc cgcccacgcc
    12841 ctggtttctc tcatggcgta tcaggataat gcgggggtct tgctgggcgg cggcagtcac
    12901 caactttaca cttgagtcag gtgatgcatc gtccacaacg atgatgtgtt ttatgtaggc
    12961 aggcaggtct ttcagcacgg ttgtgatggt gtcctgcacg cgataggcag ggatgacagt
    13021 tacaataccg ttggtggaaa aatccataaa taagattgta cttgaaaaag tgatagaatc
    13081 agtcaaagga aaaatcatgg ccaccgggga acgaattctt cttgttgaaa gtgatccaga
    13141 tatcgttgat ctgatcgtca ggcaggcatt acaaccgctg ggatataggg tgggtacagc
    13201 gggcgatgcc gcctctgcga ttaaactctc gatgcagact cccccggacc tgatcctcgc
    13261 caatttgaat ttaccaggcc tgagcggaaa agacatgctt gccgcgctca gtgcgcaggg
    13321 cgtgaaatcc cccgtcattg tcattgcaga aaaaggacag gaaaccgacg caattcaggc
    13381 gtttcgcctt ggcgctgcgg atgtcatctt ttggcctgtg cgcgatgcgg aggtcgtttc
    13441 catcgttgaa cgcgctctgc gtcagaccca gcaatcccgc gaacgtcaaa aattagatcg
    13501 tcaactctcg gccatgaata ccgagttgca gcgccgcctg cgcgacctga ccaccctgct
    13561 atccaccgcc aaggcggtca tctccattac cgatcaacgc ctgctgtttg accgcattct
    13621 cgaaagcgcc atgcaagtgg ccgaagccga ttattgctgg ctgaccctgc gcgaagagaa
    13681 aggcaatgcc tatttactgc gcgcgcatcg caacctgccc gaggcctggg ctaaaaagat
    13741 gaaccagcct ttggatgacg gcatcagttc tctggtcggg ttttcgggcg agagtttggt
    13801 gatgaacggc tcgcctttgc agaaattcaa aattgcagca ttgggtaaat cggtgggcgt
    13861 cattccgatc aaaattcagg gtgaggtcat cggcttgctg atggtggctc gcaaaataga
    13921 ccgtgagatc gcccgcgatt cacagaccat gcttgaagcc atggccgatt ttgcatccat
    13981 ttcactggtg aatgcccgtt tgttccgagc ccttgagcag agcgccgaaa ccgcccgtat
    14041 gaacgaaaaa cagcgccatg ccgcccttga cctggtgcgc accaatatcc gtaatgaaat
    14101 tcaatctgca gggtatccgc tcgaccttgt gttgaccgag accccgggtc cgctcacccc
    14161 tgaacaaaga aaggcacttg aacaggttca agctgccttg caacgccttg ccagctcctc
    14221 agagaaaacg accatcccaa ataaatagca ggcatctccc atgtcaaaac aggaactgat
    14281 cttattagca atggacgcat cccccgtttt ggggttgatg gagcgtactt tgcgttcggc
    14341 tggttttgac gtggcgattg cccacgactt ggtagggttg aataaatgtc tgcatgaggc
    14401 cattccatct ttgatgatcg tcggcgagaa atttgtcgat caaggcggtt ttgccattgc
    14461 aaaagaaatt ctcgaacgct ttcccaccct gccgatcatt ttttatgccg agcaggatac
    14521 caccggttat gcaaagaccg cactgaagat gggtttaagc ggttacctct acccgccgct
    14581 caaggtgtcg gatattaccg atgaagttca tcgcagcctt gaacgctcca gcaaactggg
    14641 cgattggctg cgccgtgaag tccgccgcac aacctcgtcg ctgacggaac gggccaaact
    14701 ttcggaaagt gaacgctcca agctggatgc gatcattgcg aatattcagg atggtgtcat
    14761 cgttttggat gacaggaagt tcatcctgct tgccaaccac gccatccacg atatttttaa
    14821 tttccacgat cgggaaatcg tgggcaggcc gcttgcagac gccataaaaa acgcggacct
    14881 gaccgccctt ctcgcccggg caggggacag cccgctgaaa tatcatgaga tcaattttga
    14941 cgatggccgt gtgttcaatg cccaatacac gcccatccca aagatcggcg gggcgatcac
    15001 catgcaggat atttcctacc tcaaggaatt ggaccgcctg aaaagcgatt tcattcacac
    15061 tgtgtcacat gacctgcgtt ctccattgac ctcggtcttg ggatacaccg aactgatcga
    15121 acgcacgggt ccactgaacg ccaatcagtt ggagtttctt caccgtttgc agggcagcat
    15181 tcaacacatc acgactctga tcaacgactt gttggatttg ggccgcctcg aagcagggtt
    15241 tgatacgcgc cgcgaggttg ttcagttaag cggggtcttg cactattcgc tggatatgtt
    15301 cgacggccag ttgaagaaaa agaatatcac ccttgtcacc gatgtggccc cggatctgca
    15361 cccactacgc gcgaatccga ttcgcatccg ccagatgctc gataacctga tcggcaatgc
    15421 tatcaagtac acaccggatg gcgggcgggt gaccatcaat atgttcatgc aagaccagca
    15481 agttgtcctc aaagtggaag acacaggcta tgggatccca cctgaagaac aaagccgtgt
    15541 gttcgaaaag ttctaccgtg ccaccaatgc catagaagac attgagggtt ccggattggg
    15601 gctggcgatt gtcaaatcca ttgtagatag ccaccagggg cgtgtgtggg tggaatcagt
    15661 tgtggggaag gggtcagcct tcatcgtact tctccctgca cacgaataat aaaacgatat
    15721 tctgcttaaa catgctgccg ccggtgattt caccggcggc agctttcgcc aaaggaggag
    15781 acaagaaagg tattttggag gtgtctatgt tccgtctccg tccttataat agtccacttc
    15841 ttttttttca gccattcacg aatgtcacat tatggttgtg acaaacttca caaatatttc
    15901 ataggctgtc aagaaaaaaa cgcgcctgcg agatcgcttc ttccacagtc ccataggctt
    15961 gcgtggtgat cttggggtct gggttgccac tgagcatttt cgataccctt ctatctttct
    16021 ggtaaatgca caagacaggt ttgccaagac ccagcgcaaa ggcgatctca tagcccaccc
    16081 catgtgaagg tgtgctgact tcggcgatca gggtgtcgca attcctgatc cagttcacat
    16141 cgcgttcata cacatcctgc ggcgtgagtt ggcgttcttt ctccgcgatt tctgattgaa
    16201 ccaggtgtga agttgggatc tcatgaccgt ctttttcaag ggcggcgacg atcttctgat
    16261 aggcggtttc aaattcccgc ccgccagtta tggaacatgc gaaataaata ttcatttgtc
    16321 tttgttgggg aggcgagaaa gaatcgaagc gttccgtttt tgctgggccg agatcggccc
    16381 tgtgccggtg cgcaggctgt ccatttcaga gctgaatttc atccagtcat ccacaagaat
    16441 gacaatgtga ggcgatttat ctttgaaaaa gagcggcgaa aggaacagtc gcaattgcga
    16501 ttgcggcatg gggtggctgt tcatttcgat gacgatcgcg gtcattttgg caagaggttg
    16561 aatgatttca gcgcgccagc ttgaaaccga gttgggagga aataaagtgc ccatgttggc
    16621 tgaggtcgca cgccgcaccc tgcggtaaga aatgttcagc cgtagaaaag gcgtgaccag
    16681 tttcaaatac tccctgaacg gctgcacata ggcgcttttg cgcatgagcc acatcccaag
    16741 cccgatgaac acatgcagcc cgccaatgct ggcgagcgca agccagcgcc attgatccat
    16801 gccccagcta tagaccgccc aggccaaccc gagcagggca agacccaaag tgaagacggc
    16861 ggtccaccaa cgtttgatca tgtgggtgta gatgacaagc ggataacggc gtccgccttt
    16921 ttcttttgcc atgggaaatt tcgcttcctt cttaatctga cggttggtcg tgattcaatt
    16981 gagcactcag cctgctgatg gcttgtgtga gatgttcacc gcgcaggctg aaggtgatgt
    17041 cgccgcgtgt tttttcgatg atgctgcgtg caagatcggt ctggcggcgc aggccgttgg
    17101 tgatggcatc gggataatcc atggtgacca gcactgagta gatttcgtcc atgtagctga
    17161 gcaggcgttc cgcttcaggt gaatagccat ggcggaggat atccagaatg cggcggcgca
    17221 attcccctac gacctctgcc atcccgttga gaaatgttgc aggttcaacg accagttctt
    17281 cgggagtctg aagcggttca ttcctgatca aggcgcaggt gacattggct tcaacaaatt
    17341 ccttgagggc atcctgagtg tatcccgcat aaaaaagatc ggggtaggcc gcgagggatg
    17401 cgcgcaatgt gtcggcgagt tgacgtgctt cctgcaattg cgcgttcatc acgtccgctt
    17461 cgtcacggtg tacggcgcgg atggcaagcg agcaggcgcg ggtaagttga cgggcctgtg
    17521 caagggcatg gtcgcgtgcg gaggtgcggg cgtcaaaggt ctttcgaatt ccgtcggtga
    17581 tctgttcaag cttgtgcatg attaaggttc tttcggcggt tcgggtggtt gttgaaaggg
    17641 gcggaagaga ttatctgcca gtgagttgtt ggatgggatg ttgatttcac caaatgcaga
    17701 ttcatagcgg gcgatgtttt cagtgagtgc gcggacaagc agtttggcgc taagcggggt
    17761 catcacaacc cgtgatttga tcttggcgcg cggttcgccg ggcaacagat gggcaaaatc
    17821 aaggacgata tctgcaggcg agtgcgcaat gcgggcaagg ttggcatata ccaactcaag
    17881 atcggcgggg agttcgagga tggggcctgt aggtttggat gggattgggg gtgtggtcat
    17941 gggaagttcc ataatatgag aggcaggtca tgcgagcaag acctgcctcc tgtgtgggtt
    18001 ggttagaagg ggatatcgtc ttccggggga agttcggcca tttccatgcc gccaccgcca
    18061 gcaatgggtc cgctttcgtg ggcttcgcct cgtgaagaga ggaaacgaac tgtggaggct
    18121 gtcacttcaa atgaagctcc tgcagtgcca tcctgtttgg accagatgcg cgggccgccg
    18181 gtgtttttat cgggggtgag gcgtccttca acaagtactt tggaaccttt tttcacatac
    18241 tggttgcaga cctcggctgt ttttccccat gttgaaacac gaaaccagat ggtttccttt
    18301 acttgttcgc cgttccctgc agtgtattga cggctggttg ctactgaaaa agaagtgaca
    18361 gcctgacccg atggtgtgta acgcatttca gggtctttgc cgacgttgcc tacgataatg
    18421 atggtgtgat acatgggggg aaatctcctt gtggttgagg tacccgccga aattcggcgg
    18481 ggaaaatacg atagctcgta ttttacacca ctaccctatg gattgtaaga aagcaggcaa
    18541 aggaattgtc ttaagcgcat caaaattacc acctgagagg aatgccatgt ctgaaacaac
    18601 tccgaaacga taccacccat tgcatgttgc gatccattgg ttgatggcct tgctggtttt
    18661 cctgatgctt ggtgtgggca aggttgtcat gcccgggatt tcccccgaag accctcaaaa
    18721 agtgatgatg ctgcagagcc atacctatat tggcggtgca atcgcagttt tgtttgtgat
    18781 acggctgatc ctgttattta ccacacgccg tcctgcacct gcggatgcag ggaatgcctt
    18841 tttgaatttc gttggaaagg cggttcactt gcttttgtat ttgttcctgg cggggatggc
    18901 tgtcagcggg gcagggctct tccagatggc aggtttgcca gacgtattca gcgcagccaa
    18961 gccttatcca tcaaacttct ttgattatct gccccgcatg ggacatgggt tgttgtcatg
    19021 gctgttgctg gctttaattg cgcttcattt tggcgcggcg atgtatcacc agttttttaa
    19081 gaaggataat ctcctggctc gcatgtggtt tggaaaataa gcgcgtggtg aagcatggta
    19141 aaaaaagacc atgctcttta ttcggttgaa atcgtatgaa ttggcagatc acttatattg
    19201 cagacgaaaa tctcatctat gtgaaaacca gcggagttgt aacctctgag gctgccaatt
    19261 cgatgttgaa ggaaattgtt gaggtggcgg tttgtcatca atgtgaccgc cagatcattg
    19321 accacagtgg tgctgacttt gctttgaggc tgttcgagtt gtacgagcgt ccttcgatca
    19381 atgtgcgtat cgggctctcg cacaaatgga agatcgccat gattttcagc gaacttactc
    19441 aaaacacctt ttttatggaa accgttttca ggaatcgcgg ctataacttc cgccaatttg
    19501 ataatctgga aaaggccaga acttggattt tgagtgaata aaaaatgatt cactctaaac
    19561 gtaaagaatc agccacgaat tgcgcggaac gtcgcgaatt tttcaaaagt ctgaaagatt
    19621 ctacggatac gcagagaaaa taattataaa ctccgtccgc aggaggctcc gtggttcaaa
    19681 atggcctttc aaagacattc cttgcaactt atgcaagata cggacgagat ataaaaaagc
    19741 cgacccagta aaatgggtcg gctttttttg cgatattttt tacgtgctgg tgcgggccag
    19801 ccttctcata gccaccattt gacgaaggtg atccacttcg tgggccagca tcaagaggat
    19861 ccaggtttgg tagttgcata tcccaaaagc agaactgatt tcagtctttt gtgggtcatg
    19921 ggcgtggcgg gtttgttcaa tggtcgcgtg gattcgttcc gtggcggtgg cgagactcac
    19981 gcgcaggtca ttggctgaaa cattttgccg tgcgccttcg ccgaatccac gggcggcttc
    20041 caccatatgt ggaagctggc tggtggaaat ctccagcaat ttgttgtgat aaaaaccctg
    20101 ggtgtagata agatgggtga tcgattcact cagagaccaa agcgaatggg cttgtgaggt
    20161 gtaggcgacc tgggcatcgg tcagtggggc gaggttttcc tcggttcgtt cgcgggtgta
    20221 gtaaaaggtt tcgatgagct catcaagcga aaatctcgag gccgcctcca gccacgtgat
    20281 ctccttggta aatgcggaga ggatgagggg atttaatact tcagaagtga tcatggttaa
    20341 aagacgctgt atctttctgc gatgttaccc agtctcacag gagtacagga tggtttccat
    20401 ttcctgcggg gtaagtccgc cgtgatggcc gtaaaaattc tgttcgaact tatccttttc
    20461 ataccaccag acagactcat agcggtaaga cagaatcacc agattcccaa cacgttccaa
    20521 aaaacggggg gatatttctt tgccaaaata gccatctgca atcagggctt ctgttttaac
    20581 tacatcggcc ctgccttcca atcttctggc aagaaagtct tgtgcttcat ccaggaaccc
    20641 atccttaatg tagaggaaca tgtcacgggc cgagccagca gggacgatca gccggtcttt
    20701 gcgattcttc ctgatcaggc gttgaatgcc gtcaaattcg gggtgggtgt tgaggaagat
    20761 cgtggtcttt gggtcgatct cgcacatgcc gtggtcggca gtcatcaaga acaggattct
    20821 ctttttgcca ttaaagatgg tttcgaaata atgctccatc attaatagaa aggtttggat
    20881 ctctgcttcg gtttgcgggg cgttgggacc atattcatgg gccagtgcat cgatcttgtc
    20941 aaaatacagg tggatgtagg tggggttctg ttgtttttcc aaaagcagac caacgtttac
    21001 gagcgcctct gaaagcgttt tgaacgcatg cggctccgag ccttccatta ctacactgga
    21061 ataagtggag ggagtgtaat cacgcaggcc aaagttgaaa ctttccacac ccattttggc
    21121 aagttcggga tagaggatgc cttttggata aatgcttgcg ggattcactt ttttgttttt
    21181 cagattgtcg cgatccctgg tgcctgcaaa ggaaaacaac agcggggtga tgatgtggtc
    21241 cacctgcggt tcgtagtact gccattcgaa tacgccactt tctccgacgg gcaggcctgt
    21301 atgcatggtt gtgacatgtg cggcagtggt ggacggaaat tgagaggtca gtttttcgat
    21361 cttccctgtt ctctcgatgc gtttgataaa aggggcatct tgaaaccgtt cgtaaaaacg
    21421 ccagccgaag gcatcaataa agaacagtac cactgcatcg tatttcttcg acgcgaatgc
    21481 ctcaatgatc cgattgggtg tccctgcaaa accgcctgaa tcataacgag gtttgacgaa
    21541 agttttgttc attgacctgc ctttcgtgag tgtgtttcca ttgaatggat gtttattcaa
    21601 aaataaattt ggtcgggttc tcgaagaact ttgtccatgc caggcattgg cgcggtgtga
    21661 agatgtataa accgccttca tcccattgat cgggtttcgg ggcgtatccg tcggaggcgt
    21721 atttggcgca gtagactgct gatagtcgct gggccagttc caggtcaggt ttggaagcgg
    21781 gggttgaagt cccttccatg atgaccacgt catttccgct ttcaaggtgc agggtcgtgt
    21841 tcggattctt ttccaaatta cgggcatgtc gcgtttcagg gctgccatcg taatacaact
    21901 tgccatcgag gaaagcgccc caacgcggaa caacatgcgg acggccatca gggtacacag
    21961 tgcaaagcca atagttgatc gactcggtca accgtttttc aacatgttcc catggcacct
    22021 cggcgatggg atgatctaca tagccttttg gaagtttggg gcgggagatc gttggcatgt
    22081 ttattctcct aataaataac ggtcgaagaa tgctgcgacc ctgttttcat attcgaatgt
    22141 gcggcgcgtg tcgaattcca cgtggccgag tttttcatca aaccacagtt ctttgggttc
    22201 gcctgcggca ttgtagagac gttcgccgct gacgggggaa atgacggtgt cacgcccgcc
    22261 ttgcatcaaa aataccgggc gcggactgat gagcggaatc cattcggtgg cattaatctc
    22321 ctcgatctta atacccgttt gctgttcggc ccaaaagacg atcagcggca cgaaaggaaa
    22381 ttctgacaac cccgtgaaat gcttgacgct ggttgccacc gtatcctgca tggacgagaa
    22441 agcggaattc gccaccacgg ctttgatgtt ttcgttttgt gatgcaaatt gaatggccaa
    22501 cataccgcca taggaattgc cgaggatgcc gatcttttcg gggtcgattt cagtgcgggt
    22561 gagcaggtat tgataccagg cttccatatc ctgcacttca tacgcgccaa gcgtgatcaa
    22621 ttcaccttca ctttcatcgt gagcgcggat ggatgtgacg agtacattgt agccgtggcg
    22681 gtgcatcatt tcggcttcgt tcaataattc agagcgtgtg gatttgtagc catgctgcat
    22741 gataatgacc gcatcattct cgccggggat gaaccagccc gccaactcca tgtcatcggg
    22801 gttggttacg gtcacagatt cgaattcgag tttaagatca tcgggggtgt cgatgggcag
    22861 tttgcgggtt tccatggggg agtggagcag gttgtaggct tcgctgcggg tcttcaacaa
    22921 cagccctgcc gcaacaacga ccaacagtcc caaaatgaca cccaaaacga tttttgtaat
    22981 tcgattcatt ctctctccgt aatttattga aatgctgatc cgctattttc ccaaggcttg
    23041 tttcacggct gcgtacacat ccactacgcc atagccaaag gcgttgctgg ggttgctccc
    23101 cgtaaaacag ccgctggaga tgttacctgt atatggtttg gctgtatcaa tgataagctg
    23161 ctcggttgcg tcaatatcac cgatcaattc aggttgagcc gaccagatca acgccaccac
    23221 gcccacgata tgcgggcctg ccattgaagt gccgctgaat tcggcataat acccgccagg
    23281 gatggaggat aggatgttta cacccggggc aacgatatcg ggtttggtgc gccccgaacc
    23341 atccgcagtg acagggccgc ggctgctgaa atcggccatg ttgccgagaa tgtcaatggc
    23401 gccgaccgaa aagaccgaat cgtacaacga gagcggcgat tcaactgttt cgcagtttgg
    23461 gccatcgttg cccgtggaaa ccaccacgaa aatgcccgca tctcgtaaat tgttcgccgc
    23521 atacaacaat gcattcgggt cacagccttc gatggatggg cacccccagg aattattcag
    23581 cacatgtgcg gctttggttg ggtcaccatc tttcaacggg tcaccgccat gcgggaaggg
    23641 tgccagcatg aactgcatac aatcgagata caaggcgggg ttggcaaggt tgcggtccaa
    23701 attcacacaa ccgatccatt gcgcaccggg cgccacgccg atcccattca cgccgaggat
    23761 cgtgcctgtg gtgtgtgtgc catgaccgcc ttcatcattg ggtgaggtgg tgccatccca
    23821 agggtcaaac cagttgtaat catcgccgct gttaaaaccg cgatactgtt cacggatggc
    23881 ggggtgattc ccatccaccc ccgaatcaga ctggccgacc acaatgcctt caccagtgac
    23941 tccaaattcg ttccacactt gatcggcgcc gatcatggcg atattccact gcgcgttcga
    24001 ttccacatcc atggcacctt cagacgccgc agcatcttcg ggagcagcac gcaggcgcgg
    24061 gctggggatg actcggtcca cttcggggcg ggtcatcaaa tacaggcgca ccaaagttcc
    24121 gccgcgtacc tccatcgcat tttccagata atacggtgtg tattcgatgc ctgccttgtc
    24181 gaaaaaggca cgcaattcag attgcgtttc gttcgcgtgt gcggtcaagc gggtataggc
    24241 ttcggtcagg cgttcatcgc ggtctttaat ttttgccacg tcagaaagat cagcctgatc
    24301 tttgaggatg acgaaaagac ggtcgccata acttccagga ttgccaaaga tgaaaaatac
    24361 agcgacgacg agaatccatg caactccagc ccctgcccaa ccgatggcgc gcttcgttac
    24421 ggaccgtgag ctggtggctg ttacaaaacg cagaatcagc gcgacgatgc tgacgcccca
    24481 accgatgacc attacccatc cgcctgcact cattgccagc cctccaatat caccgaggac
    24541 aaaggtcaac tcagtgggat cgaacatggc gagtgtgaca aaggtgagca tggacaccgc
    24601 ggcgcctgcc gcggctctgg aaggcatgac tgcagcaacc gcaaacgcga aggacggcag
    24661 agccgccagc aaaagcaatt gtgctccgtc atacccgagg gaagatccaa gcaaggctaa
    24721 aaccgcaccg attccaattc catccagaaa aacattttct gtgcttgatt cgatcaagac
    24781 agccgccagc aaaccaaatg cagcacttgc gaatagactc aagacaacat cacctgccag
    24841 cccaagcgag ccatagatgg caaacggcaa tgcaccaatg ccagccacca ccaaaccgaa
    24901 tggcagggag gcactatccc atttgagttt gttcttgcgg atcaaaatga caacgcccgt
    24961 gccaattccg cacaaaagca tttgtgcgat gaaccccaat tggtcgttgt tggggccgat
    25021 cgcacgcaga gccagggctg ggaaacccat cagagatgca ccgagcaagc ctgcatatac
    25081 aggtttgaag cggtcgtctt tggtgaagta ccacagaagg gcggtcaaaa tgctcatcaa
    25141 aatggcttgt cccaacagac caagaggtcc tgcccaggca aagtcttttg tacttgatga
    25201 aatgatggcg ctttgttcgg tggaccacaa aacgaagtga taaatgaaca gacagaatgg
    25261 catgggcagc gcaaagaaca ggaacaggat caatgcgcca gtgccgcgat tctgcggttc
    25321 aggagtttgt tcgacgggag gggtttcttc agtcatggaa ttctcctttt tgataaggtc
    25381 agagtgtgga ttttttgtcc gcagatggtt gaccgcggtt aaagtgtagg cggggttcgc
    25441 gccaaatcaa gtcaactgtg gaaggtgggt tattaaatta ttttgtggag ggtgtggtta
    25501 atcttctggt gcctgcggaa cgagtgcttc ggggccatga ggttgcacat ggaaaatttt
    25561 gaatccccat tgcaccgagg gcccaaccaa cagggcatac accaatgtgc cgatcccaac
    25621 ggggccgccc aacaaccagc caatggttac cacagtcact tcgatgatgg cacgtgccac
    25681 gcggatgctg atccctgtgg tgcgtttgat cgcaagcatc atgctatccc gcggacctgc
    25741 acccgcatcc acgccgatgt agatggcgct tgcaattccc attaacagga tggccagcag
    25801 cagcattcca ccctgtacag gcaggttgtc tttaacggag ggaatgatgg agagccaaag
    25861 gtcttcccag gggccgatgg ataaaatgtt ccccaaggtg ccccagccga tcttttcacg
    25921 catgatcaac gcgcctgaaa gcacaataaa gcccatcatc acagtcattg tgccaatgga
    25981 aataccgatt ttgtgggcca gagccacttc caacacggcc caggcgcttg tgccgaggtt
    26041 gccctgaatc atcaagacaa tggcaaggcc aaacaacatg aagccgatct cgatacgaat
    26101 aaaatcacgg aaaaacgtat tccatcgtac gggtttaagg atgtttttca aaaattgtct
    26161 catttcaaga tgataccatg atgtgataga ctcgccgcat gacacagatt attgaagttc
    26221 aacgtgagca attcaccatc agcactgacc ctgcgcgctt gaaccttgaa accatcctcg
    26281 attttttcac ccgcgcctac tggacacaag gccgcccgcc tgagcgtacg aaacgggcga
    26341 tcgaaaattc actggttttc ggcgtgtacg aaggtacgaa gcagatcggc attgcccgcg
    26401 tcgtcagtga tctttccatt tttgcctatc tgtgcgatgt atttattcat gaagattacc
    26461 gtgcccatgg gctcggcaaa tggatgcttg agaccatcat ggctcacccc gacctgcaag
    26521 gtatccgccg ctggactttg gtgacacgcg atgcgcacgg gttgtacaag caatacggct
    26581 ttgatgcact cacgaacccc gatggctgga tggaaatgat ccgcccgttt ccgggagagt
    26641 agcagggatg cggatctttt ggtccgtgtg gaaaagagca atgaaaatcc cgcaatacag
    26701 gctgtattgc ttttggtaca atcctgccca tgaaaataaa aaactggcaa ttctggcttg
    26761 gggtgttgat cagcgtcatc tttatatggc tttccctgcg tggacttaaa ctcaatgaat
    26821 tttggggcac ggttcaaaag gcaaattacc tctggttgat cccggggatc ggcgtgtatt
    26881 ttgtcggcgt ttgggtgcgg gcctggcgct ggcattacct attgaaaccc atcaaggaaa
    26941 tccccacgaa aaccatgttc cctgtcacaa ccattgggta catggggaac aatatctacc
    27001 ccgcacgcgc aggcgaagtt ttgcgcgctg tgatcttgaa acgcaaggaa ggcgtccctg
    27061 tttctgcgtc attggcgacc atcattgttg agcgcatttt cgatggggtg gtcatgctgg
    27121 cgttcgtttt tgtcaattta tctgagctgg ccaaattgac gggtgcttcg ggtttcgtgg
    27181 gcaatattca acaagtggcg atcatcggta cgggggtttt ccttggcgcg ttgattgtct
    27241 ttttgctggc ggcgatgttt ccgcacacca cggcaaagat cggtttgtgg ttgatcgaac
    27301 acctgacccc aaagcgactg catacgaaga tcatcggcat catgaacaaa tttctggatg
    27361 gacttgcttc gctgcggtct cctcttaacg tgttgatggt cttcctgacc tcggtcatca
    27421 tctggctttt ggaaacgggc aagtactggt tcgtaatgca tgcgttcagt ttcaacgttt
    27481 cattttttgc gttgatgttg atgaatggta ttgtgaatct tgccaccacc attccctcag
    27541 ccccgggata catcggcaca tttgatgcgc ccggcattgc cgtattgacc gcctatggcg
    27601 tggatcaagc cactgcagcc gggtacaccc tcaccctgca tgtcgcgctc tggctgccca
    27661 tcaccatttt gggcgcttat tatctggcgc gtgaaggcat caaatggtcg gatactctgc
    27721 gcgaagaaac ggaaaatacg gattgataaa atgacaaccc gtatttacgt tgactggtct
    27781 ggagacccgg gatttaaatt tcgccaaggt tcgtcagaac tgctcgttat tgcagctgtt
    27841 atggcggatg aagaacccaa tataggaaac cttcgtgaaa aattatctct tccggataat
    27901 tatgaatttc atttttcaaa agccgatcat cgtatccgtg agcattttag gaattatctc
    27961 aataccgaat tggaaatacc cggtgtggtt gtgcttcggg ccgataagca aatgttttca
    28021 ttgaatatgc gtcagaaaag gggggaacaa gtcgttgctg acttgattgc ccaatgtgtt
    28081 gttaatttgc cagccgaatt actgcatgac tctactttat actatgatgg agagaaagaa
    28141 caagaatctt tcaagaacac tttacgggta acactatccc aaacgcttca ttctggagtg
    28201 tttttgcgta gtgtaaaagc tgtgcctgcc agtcggaatg atggtttaca ggtggccgac
    28261 atgcttgccg ggtttgttcg taataatcct tcagcgataa aatcaagtat tgtcaaactg
    28321 atccattacc ccgattaaat caaaagcgcc tggctagttg gggttgcttt tatacaggct
    28381 gacttacctc acctgaaacc caacccgcgc gattgcgcga ctacaccagg ctgctgggtt
    28441 catattacac cttcaagaaa cgagagtcaa ggaatgaaaa tagcaattgt cggagcagga
    28501 tacggcggca tggttgccgc atgggatttg aaaaaagcgg gtcacgatgt gaccattttt
    28561 gaatcggccg aatatgcagg cggacttgcc agcggtttca aggaacccca ctgggattgg
    28621 tcagtggaga agttctatca ccactggttt caatcagatg gcgaaatgct tgggttgatc
    28681 cgcgagttgg gcttggaaga caaggtccgt tttccgcgcc cgttgacggt gatgtactac
    28741 aagggcaaat ggtatccctt tgactcgatc ctcaatgcgc tgcttttccc ggggctgggt
    28801 tggggcctga acaagatccg ttttggcttt gtcgggctgt tcctgcgcct cacgaacaat
    28861 tggcgcgcat tggaaaaggt caccgcggat gagtggatga tgaaatatgc gggcaggcag
    28921 gtctacgagc agatgtggaa accgctgctt gttggaaaat tcgcgtcctt ttataaagat
    28981 gtcaacatgg cgtggttgtg ggcgaggatg catgcacgca ccacacgtct tggtacgttc
    29041 gaaggcgggt ttcaaaagtt tgccgatttg ttcgcggaga aactgcgcgc acagggtgtg
    29101 cagatcaaac ttggggtgag catcaaatca attaaacggg atcaggcttc agggagtctg
    29161 ctcgtggacg atcagccttt cgacaaggtg ctggttactt cgtcgccgag cctgatggca
    29221 aagttatgtc cggacctccc cgaaagttat ttgaagggcc tgctggggtt gaagtccatg
    29281 ggcgcggtgg tgatgacgct ctcgcttgcg cacccgcttt caaaggaagg ctactactgg
    29341 ttcaacgtgc cgaaggagga gggctatccg tttttggcgc tggtggaaca taccaatttt
    29401 gtgccgaagg aaaatttcgg cggcgatcat attgtgtatg caggcgatta cctcgagctt
    29461 ggacatgaat atttttcaat gagtgatgag cagctgctcg aaagattcat ccctgcgttt
    29521 caaaaattca accctgagtt ctcccgtgat tgggtgaaga agatttgggt ccacaaaacc
    29581 aactatgccc aacctgtgcc attggtggat cattcaaaga acattcccga gatacagaca
    29641 ccgatcgaag ggttgtattt cgcttcgatg agccaggtgt atccgtggga tcgcggcacg
    29701 aactttgcgg tggagatcgg ccgcaaagct gcgcggatga tgaagtaatc tcccataccg
    29761 cagacttcag tctgcgagtt aaatataaaa cacagactga agtctgtggt acttaaaata
    29821 gacaaggcaa tactctttca tgtggttcta tctccttcaa ggcattgggt tcggattcgc
    29881 cgccgcatca cagcctgggc cgtttcaaac ctatttgatc tcacagaccc tcacacgcgg
    29941 ctggaaacac accctgcccg ctgcgctggc tccgctcgtc agtgatgggc cgatcatttt
    30001 gctgtgcgtg ttggtgctca gccaggtgcc tgcatggatg cagcgcgggt tgtacattgc
    30061 aggcggcttg ttcattattt atcttgccta cggcacgtat cgaacatggg agaattttga
    30121 cactgcggcg caaaccaccg ctcccgtggg cgaagttcaa caaagcgttc tcaaagccgc
    30181 gatgatgaat gcgctcaatc ccaacccata tattttctgg acgctggtca caggtcccat
    30241 tttgttgagt ggatggcgtc aatcaccgat ccttggtgtt ggttttctgg ccgcgttcta
    30301 cctcaccatg atcgcagggc tggcggccgt gatccttatt tttgggtttg cacaaaggtt
    30361 cggcgccaaa ttcaaccgcg tgatgctggg catttcagcg atcgccttgt tttgttttgg
    30421 catttatcaa ttgtggctgg gtttgtttcc aaaggagatc ctatgaatat cgttcttgtc
    30481 catgtgcatg tcaaacctga gtttgttgaa gcctttaaac aggcgagcat tgaaaatgca
    30541 tcgaacagca taaacgagga ggggatcgcc cgcttcgatg tcattcagca aaacgatgac
    30601 ccgacccgct tcattcttgt tgaggtgtat aaaaccgccg aggcctctgc ggcgcacaag
    30661 gaaaccgcgc actatgccca gtggcgcgac acggttgcag agatgatggc cgagccgcgg
    30721 cagggaatca agtacgcgaa catccatcct gaagactctg gctggtaatt tatgcctgca
    30781 tttgaatttg ccaccgcaaa ccgagtcatc ttcggggcgg ggaaattaag tgaacttggc
    30841 aatcaggtca aggggcgtgc gaagcgtctg cttttggtgc ggggaaattc atcggatgcg
    30901 attccgcgtg tgaaggagat tctctccggg caagacatcc tctttaatga atttgcgctt
    30961 catggcgagc cgacagttgc cacagcctcc gagggagttg accttgcgct tcaacatggt
    31021 gtggacatgg tcgttgcact tggcggcggc agtgtgcttg atgcaggcaa agccatttca
    31081 gcgttgacga cgaatcgcgg cgacgttttt gattatcttg aagttatcgg caaaggacag
    31141 ccgctggtga atgcgccgct gccgttcatc gccatcccaa ccacggcagg gacggggact
    31201 gaagtgacgc gcaatgcagt gcttggctcg cctgctcatg gggtgaaagt gagtttacgc
    31261 agcccgatga tgctgccgtc gcttgcgctt gttgaccccg cgttgacata tactctgccg
    31321 cccgcgatca ccgcgagttc tggtttggat gcgttgacgc aattgatcga gccatttgtt
    31381 tctgtgaaag ccaacccgat gacggatgcg atttgccgcg aagggattcg tcgtgcggca
    31441 aggtcattgc ggcatgcctg gcacaacggg gcagaggttg cggcgcgtga agatatgtcg
    31501 ctggcgagtt tgtttggcgg catggcgctg gcaaatgccg cgctgggtgc cgtgcatgga
    31561 ttcgcgggtc cgctgggcgg gatgttgaat gcaccccatg gcgcgatctg tgcaaagttg
    31621 ttgccgctgg tgatggaggc gaatctcaag gcgttggaga gccgtcacgc aggccatgtg
    31681 gcgatcgagc gatataccga aattgcacgg atgaccacag ggaatgaatc tgcttctgcc
    31741 tgggatggtg tgcagtgggt gagtgatctg gtgcgcgagt tgaagattcc gccgctttcg
    31801 acgcatggaa tgaatgcggc tcacattccc gaagccgttc aaaaaacctt gaatgcgagc
    31861 agtttcaaag ggaatccgat tccgctgaat gagggggagt tgcgcgagat tttggagaag
    31921 gcgttgt
//
LOCUS       DAOQLC010000040        31908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_440_length_31908_cov_24.080683, whole genome shotgun sequence.
ACCESSION   DAOQLC010000040 DAOQLC010000000
VERSION     DAOQLC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 31908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31908
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_440_length_31908_cov_24.080683"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="PL000_09020"
     CDS             <1..1498
                     /locus_tag="PL000_09020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89082.1"
                     /translation="PFFSSRFAQIAAFVLLLGLGLGIRLYDLTDLPLDFHPTRQLLSA
                     LKARGMYYQTLSDIPTEQKSFAVQQWKIRAAIEPEIVERIVAFTYQFTGEQIWVAKVF
                     SSAFWVIGGLFLFLLARQLTSTEGALAATAFYLFTPYAVAASRSFQPDPLMVMLIIIF
                     WWAVYKWSEDLTSFQWAVIAGLFGGLAIYVKFVAAFFVIGGGLGSILRRESLFETARR
                     PQIYVMTVLGILPGAAYVIYGVWIAGYLGQQFGGRFIPALFLSPSYYLGWVGMLNIVI
                     GGIALMLGLLGLFFVDEKKRRFFLGLWGGYALFGMYFNYHISSHDYYSLPLIPIVALS
                     LAPLAEWFFAQLTALTPTRLTRFFAFGLLLLGMFASLWNIRAEMKATDYRPEAQMWAE
                     IGETLEDKNTAGLTQDYGSRLAYWGWTSITTWPTYGDLIYHDDLRGAQLEFDGLFEDI
                     ALKKELFLVTDFDELKRQPLLKEKLSGYPIFAEGDGYIIFDLTSTGSQ"
     gene            1499..2953
                     /locus_tag="PL000_09025"
     CDS             1499..2953
                     /locus_tag="PL000_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89083.1"
                     /translation="MTKTTKPTFDFITIATGLGAAFSAFALGVYAYLGIFSRHLADDY
                     CSVDFIRTNFFSALLNNYIFVSDRYTNFMLIALSEYISPRTVSILPALFIVLWLTGIA
                     WMLSEASRFTKNGWSKLTIVSLTFLVVFFSLLQAPNRFQILYWRSAMSTHFAPLVFMP
                     FLAAFILKVITESEGKPAPFWKYLLGFFIALIIGGFSEPTVAVMITLLALAFAATWKW
                     MSPSTRSAALKMIGWSLVGAVTALLIMGLAPANYYRVSLGKEPPTLAFLIVRSFQYGF
                     NFIDNSFRTLPLPTLFTVAMPFFMFYNRYASPTPALTESQRKLARTLLMVIPALSYLL
                     IVASFAPSVYGQSFPVERARFAGQVSLVAGLMIEGALLGSLLAQWRPAFAEKLPLKLA
                     SAVLFAVAALYPLRAGWLSLNDIPDYRARAEAWDTRVIQINDLIAQGQTDLLIVQLDG
                     VEGVKELEVTEKHWVNRCAAKYYEVNSIRTAPPK"
     gene            2956..3876
                     /locus_tag="PL000_09030"
     CDS             2956..3876
                     /locus_tag="PL000_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC89084.1"
                     /translation="MKRPILVTGAHRSGTTWVGRMLAANLQTAYISEPLNVLHRRGVY
                     RAEVQHWYQYITSENEGAYLPAFHELLNYRYHLFAEIRSIRSQKDFLRMGRDLSIFMK
                     GKLQNQHALLKDPFAVFSAPWFAKTLNCQVVITVRHPAGFASSLKRLNWPFDFRDLLN
                     QPMLMRDHLEEDRAEMESTPADDIIGQSSLLWRMIYRAVHASRTRGLIPEIKIVRHED
                     LSLDPIAGYKSLYESLGLTFDENVRDTILNSSSSENPTELSKKKVHSVKLDSRANMDN
                     WKKRLTAEEILRIRKMTESVSHLFYSENEW"
     gene            3903..4682
                     /locus_tag="PL000_09035"
     CDS             3903..4682
                     /locus_tag="PL000_09035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC89085.1"
                     /translation="MSTLPLVSIITPSFNQAQFLEATIQSVLGQEYPRIEYIVVDGGS
                     KDGSVDVIQKYAGRLAWWVSEQDKGQTDAINKGFNRATGDILAWINSDDTYNPQAVGQ
                     AVKQLMENPEVGLVYADCNYIDEAGNVIGKFPAAQTDHRRLREGYVHIPQQTMFFRAK
                     YWKELGPLDPSFYFAMDYDLWTRIAKHAPFKYVAGQTWANFRIHNLGKTTSADDRCWP
                     EMIRVHYRDGGSFFSVIVAKHYLRKVIGPLWKWRIKKQANR"
     gene            4705..5310
                     /locus_tag="PL000_09040"
     CDS             4705..5310
                     /locus_tag="PL000_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89086.1"
                     /translation="MTHLLTPPTLEDLIRLLKAWRFWMFGALVGALIGAAVYYADPPP
                     FRAQATVNVDFNLEQAWPQETDRQQFYYLERESRKLEEVAWSDEVLGQLSSSFGISVE
                     ELRRGKLQLSQPAEAGWHFYADDDDAQFAASLATTWAKAFVAKTEADVASGAINEFVR
                     LEATQSESTAKERSIPLSSYLLAGSIGFLTLAVFVLLFKRK"
     gene            5376..6845
                     /locus_tag="PL000_09045"
     CDS             5376..6845
                     /locus_tag="PL000_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC89087.1"
                     /translation="MKEFFPNSYKISRILWGAALLTLPVTSFRWFPFLGEGTLVRPLA
                     MYPLAVLIPLLLILAWRGKTKLNWSGALIPLGVLVLFMVAATSFGALIDPIPLRGQTY
                     SGRAIRALATLFIGIAFFIAAMWMNKDEDDFRFTVTWLFAGLCLDLAWSGLQAVTFYT
                     GLLEKEMVTHWQRAFSMRELVRTNRISGLAYEPAWLAGQFATTFIPFLFAAVLTNFRI
                     TRLKWLEPVLLALSLLVVLATYSRGGLLTTLAAAGLTFLFTGQLGIVWKWFIGGFRGG
                     MRGILFRVGILAAIFSVAAGAFIFLSQKNFFRRLWETDAQSLSEYIVDINAGARGAYS
                     AGALSAFEEYPWAGVGLGASGFYIYQNLPDWSLTTVPEIAKQLSPENKLYPNPKNLYA
                     RLLAETGLIGFFLFLAFQFYILGDILSLLRHREGWARFAAVAGIFAWLAITFYNFTQD
                     SLTTPNIWVIPGILVGLAASSTMPEEPLPGIGLQSKENK"
     gene            6842..7567
                     /locus_tag="PL000_09050"
     CDS             6842..7567
                     /locus_tag="PL000_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HNC89088.1"
                     /translation="MIVLSVGMPRAGSGWHYNLIHDLMKTTGCADARDIRERYRLQNV
                     LTEVNCNIGVLSARRLGLVMIPALVNTFVIKAHSAPTNASRLLSTMGRLRITYIYRDP
                     RDAMLSAYDFGQRALTKGRPNAFSHLSSFEKSVDFMMEYVHIWEKWIKEKNVLTARYE
                     DLLMNYDEESTRLVEYLKLNGSRPEVRKVIEQYRPGTAEGQQGLHFYKGKIGRFRDAY
                     NEEQKSILKEKLGPYLTQMGYNE"
     gene            7637..9388
                     /locus_tag="PL000_09055"
     CDS             7637..9388
                     /locus_tag="PL000_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNC89089.1"
                     /translation="MLRRFDSPYMADWFAISLRWIILVGFVVSLGLGDKLEIAVSWPL
                     GLLILWNLFMTALASMNIRVAYHRLFSILVDITLAGLFFWTQGGLRGPAAWAGILPIL
                     TGSIFFELLGAVAASVFFSGLMIYLGLQDGDSLPLSITFSAALIALSMVFGFLGKRMI
                     IHMRKNRELWLDSEERKSAMQAERMRAIYELTAALSSTLSYKRVLESALNLSASALNP
                     DPEQLHSDPLVGVVMLFNGGKLRIGSARRFTTADMRVTFDAAEGILKKTIDEGEPLWF
                     KDIGYDPELGRVIALRNCTSGYCFPLRSGFNVYGVLLFAHPDAEYFTTERRNLLDIIG
                     RQAVIAIQNARLYQDLVEEKERMVEIHEEARKKLARDLHDGPTQSVAAIAMRLNILRR
                     MMAKDVASAGDEVVKLEELAHRTTKEIRHMLFTLRPLILESQGLVAAIQAMADKMAET
                     YSQKVTHNIDERVTSQLEMGKQGVIFYIIEEAVNNARKHAAAETIAVNLTQMDAGIAL
                     LEIADNGLGFDVKSVTEAYDKRANSSLGMVNLRERAELVNGLFQIDSAPGKGTKVQVY
                     IPLTEEAADRLHYMRNK"
     gene            9392..10279
                     /locus_tag="PL000_09060"
     CDS             9392..10279
                     /locus_tag="PL000_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC89090.1"
                     /translation="MPIAANLYYFSHDTEDDLETKRPPIILIHGAGGNLLSWPPQVRR
                     MVDERVYSLDLPGHGKSEGTGRQSIDEYVEDVLAFMQALKLRTAIFAGVSMGAAVALA
                     LTLKHPRKVRGLCLLGSGSKMRVAPLILETAGNPNTFESAVDLINENCFSSEAPQHLK
                     DISKRHLLDVRPPVLLGDFLACHEFDVTSQLKKIRTPTLLICGAADKMTPVRYSELMQ
                     ETMPNAKLHIVENAGHLVMSEQPDVVADLMKQFVDSIPVRTRKTKKDRVELNVESDPA
                     QETESALQADSVPSTEPNS"
     gene            complement(10276..10872)
                     /gene="rdgB"
                     /locus_tag="PL000_09065"
     CDS             complement(10276..10872)
                     /gene="rdgB"
                     /locus_tag="PL000_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNC89091.1"
                     /translation="MKKLLIATNNKGKVKELQELLRELNVQLVTPAEINLDLDVEEDG
                     ATYIENAAKKAIAFAQASGLISLADDSGLEVDALDGAPGLYSARYHPKPGANDADRRA
                     YMLENLQGKPRPWKAHFHATIAIAIPGGDVKIADGNCHGEIIPEERGTGGFGYDPIFF
                     MPELGKTMSELEMDEKNRLSHRARAVINAMPVLKEIFS"
     gene            complement(10869..11927)
                     /gene="queG"
                     /locus_tag="PL000_09070"
     CDS             complement(10869..11927)
                     /gene="queG"
                     /locus_tag="PL000_09070"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNC89092.1"
                     /translation="MSDLKQAIKDKARQLGFILAGVTSCEPPAHYGMFEEWLHQNKHG
                     NMQYLAEERSRIRRADPKQILPECRSILVLALPYQPVTFNPQSSREAFRISSYALGDD
                     YHDIIPPRLQQIVDFIEEQLGHPIPNRYYTDTGPILERELAQRAGLGWIGKNSMLINP
                     QMGSAFFLAEILLGIELEPDDALITDHCGTCTRCLDACPTQCILPNRTLDASRCISYL
                     TIELKEDIPEELRPLMQNWIFGCDACQQACPWNRFSPQADPAFESKIPLPVLTSDLLL
                     TSVEFNQRFKKSPVKRAKRRGYLRNVAVAVGNTGSENDLPILEQAAQDEEPMIREHAQ
                     WAKKHVTQDIILRHRNSK"
     gene            complement(11968..12582)
                     /locus_tag="PL000_09075"
     CDS             complement(11968..12582)
                     /locus_tag="PL000_09075"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89093.1"
                     /translation="MKARFVISAIIATLMLAACAPATPIVPTPDVLAVRTSAAMTVVA
                     EFTLTAAAFTPTPMPPTPTFTAEPPTLTPTIAFATDPTQIALGTPGGPLCDDYSFDVA
                     TVDVTILDGTAMTPGQEFVKTWKIKNTGICAWGDGYGLVYTYGDKMSGVPVPIGLVEV
                     GQEVEVSVNFKAPDQIGEYTSAWQMANAKGVTFGKAIFVKIVVK"
     gene            complement(12610..13311)
                     /locus_tag="PL000_09080"
     CDS             complement(12610..13311)
                     /locus_tag="PL000_09080"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89094.1"
                     /translation="MRVRKFLWLASFLVLAMVLSSCNLGATPVPTQDLAAVQTEAFNA
                     VLTQVAAQSSPTPLPTNTLLPTSTLAALPTFAPVGGGDATPIPFNTQQPVLGITPLAS
                     PIPTAAVFATITTKNGCNDGVLVKESEPFDGDVLQRGKAFEKVFQFLNTGSCVWDEGY
                     TFAFLPDFSTPGFKGSDIVIKKAEDYVKPGANISFVLKLVTNNAPGEYIGTWKLRDDE
                     GNYFGSMVWVKYVIK"
     assembly_gap    13519..13618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13640..14884)
                     /locus_tag="PL000_09085"
     CDS             complement(13640..14884)
                     /locus_tag="PL000_09085"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89095.1"
                     /translation="MKKAPISLFVTFLMMAQLACNLGNSTATPDTFATLNGLYTASAQ
                     TLEAGTTQQVSTAAVGASATPGLPLPTYSPAPPPTGLPAATATNQPIYATAAPVSRCD
                     AAEFVKDITYPDGSPVVRNTSFEKTWRIKNIGTCTWTTAYSLVFDGGYAMSGPTTAAL
                     SKSVSPGSTIDISVQLTAPSAEGEYIGYWKLRNANGVNFGIGDKAQKAFWVEIRVPGA
                     SYMAYSFVEHFCEAEWRNEGAPLPCPGTRGDANGFVVRLDTPMMENGVTENNPSLLTV
                     PQDARQGEISGQYPAFTVKEGDHFRTIVGCQYGSRRCDVVFRLDYRNNGTVKNLFTWN
                     EVYEGNSYPIDFDLSPLAGETVKFILVVTAHGGQNQDDAIWLHPFILRQGTPVPTTIP
                     TLTFTPTFTPTPTFTATATPTP"
     gene            complement(15016..15978)
                     /locus_tag="PL000_09090"
     CDS             complement(15016..15978)
                     /locus_tag="PL000_09090"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89096.1"
                     /translation="MFKKFKYLLFTLIVVLSLLITACSGSAETPETTQQDTNSIQTAV
                     AETVAALGNVSNPTPEAPAFTPTPLLFAPTFTPLAPVASPTRTRNPSVSECAQASLVS
                     ENIPDGTIFKPGQGFTKTWEIKNTSPCSWDTSYKIIFWDGNVLGGAYVYNLPQAVDPG
                     QIVPISILFTAPQADEAYTSKWMLQTPDGVEFGVGEYSAPFYTQIVVSSSANPGFAIT
                     SVEYNVVRDPATGCPANVNHIVYATFTSNGPIEFTYYWMQSDNNNSNPKKLKMDAAGT
                     LTISREWKLHLGSTPGTKWMAIVIVDPVEQEYPHAEFTYLCGSS"
     gene            complement(16064..17035)
                     /locus_tag="PL000_09095"
     CDS             complement(16064..17035)
                     /locus_tag="PL000_09095"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89097.1"
                     /translation="MKKQIRLFNLLMIFTLFLSACNLPSNNAEDVASTSAAQTVEALL
                     SATPAIIINNTPTFTVAPPVTTATLTPIPLPVLTNTPIATATSNCNVAEFVADVTIPD
                     GTVVTPNQAFVKKWRIKNIGSCTWTGFSMVFDSGDSMGGPASKPISTVAPGQEIDLEV
                     NLTAPATLGQYKGYWRIVTNGNVLVPVVKGYQGKSFYVDVKVQAATTGSPSVTTTITP
                     TATVTSTPFVFTVTSVLFNVTGSCPNFHYTFSVTTNGPGTVTLHRVFSDGSTDPYPAS
                     LTFNSAGTQTSPSIPMTFNTPGSSAWVDVYIDSPNQKQFGRATFTCP"
     gene            complement(17368..17913)
                     /locus_tag="PL000_09100"
     CDS             complement(17368..17913)
                     /locus_tag="PL000_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89098.1"
                     /translation="MKKYIYMLILVVLVVSISACGGGNPAPTEASADSGAPVDSGAPA
                     DSGAPADPGAVVDAPADSTDGGAPDPMDGFMFPPMPGEAEPQDDVNYFVAILREQSGI
                     SGDLQLMSAVPATTTWDTVFAYYDQQAKNNGWVLEKTEEVKTQKGYLCTVALFTHGKN
                     KILIAYYPLNGVVNILQVQGQ"
     gene            complement(18052..19587)
                     /locus_tag="PL000_09105"
     CDS             complement(18052..19587)
                     /locus_tag="PL000_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89099.1"
                     /translation="MTEAKIVLEARNITKQFPGVLASDNVNFDLRKGEIHALLGENGA
                     GKSTLMNLIYGLHRPDSGEIAVNGKTIEIDSPNDSIAAGIGMVHQHFMLIPVFTVAEN
                     VMLGDETVKRGSLDRAAVATRINEISKQYGLDVDPNALVGDLPVGLQQRVEIVKTLYR
                     NAEIVILDEPTAVLTPQEAEDLFRIMRDLTARGVSIIFITHKLKEVLAVADRITVMRA
                     GRVINTVQPQGTNEAQLANMMVGRQVILTVEKDDHAASGDILKVEGLSVRDQRNLETV
                     HNVSFTVRGGEVLGIAGVQGNGQTELSEALTGLRSPTGGKVTMDGIDLTGKPPRIMTE
                     AGLAHIPEDRQRHGLVLSYSIADNMALCDYYRPPFSNRGVLQQKALDENARKLIGAFD
                     VRTPSPFVSAGKLSGGNQQKVIVARELSRSNVKLVIANQPTRGLDVGSIEYIHSEIIK
                     MRDRGVAVLLISAELDEIMALSDRIAVMYRGQIVTVVEAKKTTREQLGLWMAGAHVES
                     SPA"
     gene            complement(19597..20709)
                     /locus_tag="PL000_09110"
     CDS             complement(19597..20709)
                     /locus_tag="PL000_09110"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89100.1"
                     /translation="MDWKRFGFIALIIVVLFGTVVMIGQVKQPPVLIITSMLATTIAV
                     ATPLALGALSGVYCERAGVVNIGIEGMMLTSAFFGWLGAIYMYHVFGFGVGFSIGMGV
                     FFAILTGVLMSLLHAVLSITFKVDQIIGGTVINILAVGLTGFLNRQLFFGKGSVFGGS
                     VPNSPGVLPTIHVPLTEIFTSVCGSSASCLENLAAINRVFDQKPIAISAILLVFFSHY
                     VLFNTRWGLRTRAVGEHPKAADTVGINVIKMRYANVLIGGMFAGLAGAYFTIESVPSF
                     EPLLTNGRGFISLAAMIFGNWTPIGAWAASLVFGASQALLINMQIFRDVIPPQWSFLQ
                     ESYIVGLTPYILTMFILTGIIGKTTPPAADGVPYEK"
     gene            complement(20718..21845)
                     /locus_tag="PL000_09115"
     CDS             complement(20718..21845)
                     /locus_tag="PL000_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89101.1"
                     /translation="MQSEKQSNSLIKQILQTISDNSLVPFLAILTAVVVGGVIIAFVG
                     GNPILAYRGLLEGSFGSTKALSETAVWATPYIFAGLAVALAFKGGLFNIGAEGQLAVG
                     AVASSLVGYALPEWLGYDLPAIIHLPLAIVFGVLMGGLWAAIVGWLKAYTGGHEVINT
                     IMMNYIALNTTSYLLNGVMKDRSPTNVIARTPLIADSARIPPIFDGLRVHWGFILALL
                     VAFGIWWLLNKTTLGFQIRTVGLNPDAAKYAGINVKRIVILTMALSGMLAGLAGTIEV
                     TGLNYRHELGFSQGYGYDAIAIALLGKSHPLGVVLAAFLFAAMRNGATRMQFLTQMPV
                     DLISMIQAFILLFVAADAIVRYIYRIKSKGERVVLTRGWGG"
     gene            complement(21986..23032)
                     /locus_tag="PL000_09120"
     CDS             complement(21986..23032)
                     /locus_tag="PL000_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC89102.1"
                     /translation="MKKLYFVMALLIVASMVLTACGASAPTAEDCSKEEVFCVGLVTD
                     VGKINDKSFNQSAWEGVQKSQADGVADVVQFIETSDAKDYAKNIAQFGDAGYDVIVTV
                     GFGLGEATAAAAATYPNVRFIGVDQFQAETVAGVSGLNFPEDNAGFLVGALAAMMSES
                     HKIGAVCGTDVVPPVWRFGEGYKAGAAYADEKNGTTTEVFVVYHSDVGFDKTFTDPEW
                     GAQTAKSMMDQGADAVFGCGGLTGNGAITAAAQAGAYAIGVDTDQYLTLPEAAPRMLS
                     SAMKLITPGVADLIKATKEGSIADGNVFGAAGYAPFHDLDSSVPAEVKTMMEEINKGL
                     LDGSIKTNVAPVKP"
     gene            complement(23163..24119)
                     /locus_tag="PL000_09125"
     CDS             complement(23163..24119)
                     /locus_tag="PL000_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC89103.1"
                     /translation="MSALTACMQSQDCFSEEVFCAALVTDTRGLEDHGMNQETWAGLQ
                     EAQSGGLSDRVEYIESVDTRDYEKNIVFFAEQGVDVIFTIGPGMDDETLRSADLYPDS
                     VFVGISQPQKEARKNLIPVNFAEDQMGFLAGALAARLSGAGVVGAVCETSDIDAMWRY
                     CEGFNAGVKSVDENIKVLVFYRDDASRDDLFLDEVWGYETGQSLIQRGADVIFAAGGA
                     TGEGALRAAMDGQVLAIGAERDQSAEGYGVVTSVYGSASFEVQEVMRMIQGENLSEAG
                     IGSFGYVPLNEKFSEGLTRELDSLLSALLNGEIQTGVSREKP"
     gene            complement(24170..25450)
                     /locus_tag="PL000_09130"
     CDS             complement(24170..25450)
                     /locus_tag="PL000_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89104.1"
                     /translation="MSSRLNYGKTFLLGFGFFGLSVVWGVYNAFVPIFLSEKFGLSPV
                     VIGFFMTLDNIAALFIQPPVGAWSDRLRTPLGRRIPFLLIGAPITAVAFGLIPLPAIL
                     PLFVACTSTLLLSAAFWRTPVIALMPDITPSEFRSQANGIINFMGGIGTIVALQTGGL
                     LYKLSPNFPFWLGSGLVLLSALIVYLFIEEPKEYAASEEQPGMLASLREVVSSEDKSG
                     LRILFAIFFWFIGFSAVETFFTLYAKNHLGLNEGDGATLLSVLPLFFVLFAIPSGFIA
                     GRLGRRTTISIGLITLTIMLVLLYITPAKALLTGISPLPLVGIPLEEGGPRMLTLAGV
                     LLIFGGAAWALININSLPMIVDLTDAARIGTFTGLYYLFSTFSAIVGPNVNGWAIQLT
                     NGNYNIIMLIAPFFMMIALWLMLGVKRGEAAQAV"
     assembly_gap    25493..25592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25593..26278)
                     /gene="trmD"
                     /locus_tag="PL000_09135"
     CDS             complement(<25593..26278)
                     /gene="trmD"
                     /locus_tag="PL000_09135"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC89105.1"
                     /translation="MPEVFSSYLDTSILKRARERGLIDVRVHNIRDYTHDKHHVTDDT
                     PYGGGGGMVMKPEPVFEAVESVLGISNENDSSPIPVILLTPQGRLFNQAVAQELSAYS
                     HIALICGRYEGFDERIREHLVTDEISIGDFVLTGGELPALMLIDAVSRLLPDVLGDPT
                     GAQDDSHAMGLLEYPHYTRPPEFRGWRTPDILLSGDHAKIDKWRREQALERTFKKRPD
                     MLEKAELTKE"
     gene            26402..27103
                     /locus_tag="PL000_09140"
     CDS             26402..27103
                     /locus_tag="PL000_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC89106.1"
                     /translation="MNNYIALVITFAASLLFLRSMDFIAHRGWMDGKLSRKIIHIGTG
                     PLFVLCWFFFNDAPEARWLAALVPFAVTAQFALIGLGVIKDEASVQAMSRTGDPKEIL
                     RGPLYYGIMFVVLTLVYWRENPTGIIALMMMCGGDGIADIVGRRIASPKLPWSREKSI
                     AGMVSVFAGGWLMAGIMIYIYVAAGVFSAPFAQYTLPITLIALAGAVVESLHYKDIDN
                     VTMTLASALIGGLFF"
     gene            complement(27421..28167)
                     /locus_tag="PL000_09145"
     CDS             complement(27421..28167)
                     /locus_tag="PL000_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC89107.1"
                     /translation="MSNEQEYLPKIAMSIQAHPDDQDFTVAGTLAKWAKAGCKIISVL
                     ITSGDSGSNDVTKGADYKSVLAELREGEQLAANAVLGISETVFLHYPDGELTASIALR
                     KDLTRLIRIYKPDVVVSGDPSAWFYGSGYINHPDHRAAAEAAAYAVFPSAGTRLIFTD
                     LLEAGYEPHNVKRLYIHGSEKPDTWVDISETIDIKIEALKKHVSQSDTHDVDDWMHEW
                     AKEEGKEKGLEFAESYKVMILKKEDDIPGE"
     gene            28837..29811
                     /locus_tag="PL000_09150"
     CDS             28837..29811
                     /locus_tag="PL000_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC89108.1"
                     /translation="MKTVQPIQSFDQIKLLADARRMEILRLLMAAPATLTILAQTMKQ
                     SPAWVRHHILALESAELIEVSELRKTGKVTEKFYRAKAAAFLLQEVVLPKSKKQSIIF
                     SGSHDLALEEIAEHLNKHVTLLSMPVGSLDGLVNLRQGLCQISGAHILDATGEYNTPT
                     VRHLFPDRNVELVTLAYRTQGLIVAPGNPRNIKKISDLARENVKFINRNSGSGTRIWF
                     DNELKKLKLDPTQIPGYDQSVKTHSEAAQAVFTKTADVALGLQAAAKQHDLDFIPLFE
                     ERYDLILPRENESLLSPLLDYLQTSTFRSSLGRLVGYNPTHSGEQIAV"
     gene            29832..30041
                     /locus_tag="PL000_09155"
     CDS             29832..30041
                     /locus_tag="PL000_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNC89109.1"
                     /translation="MKLSARNVIKGKVTKIVKGAVNSEVTIEIAGGAQIVSIITNSSV
                     KSLGLKKGKDAYAVIKASNVMIAVD"
     gene            30044..30517
                     /locus_tag="PL000_09160"
     CDS             30044..30517
                     /locus_tag="PL000_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89110.1"
                     /translation="MKKLLIVLSIFLLTACGAPPTAPITAEASVTEISASAEPTAEPI
                     TEPSSPVLFKIVKADGTTFDVTLEAVKSLPLTQITVDGKVQEGPKLLEVLALAGVTEF
                     TEVSLSGTGSPSTLTFKQVDDNTILDFSNRGTMKLATTHIPKADWTKDIAEITVK"
     gene            30598..31320
                     /locus_tag="PL000_09165"
     CDS             30598..31320
                     /locus_tag="PL000_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89111.1"
                     /translation="MHYLIGIDDTDNLESRGTGHRVRQLADWLTENKLASPLGITRHQ
                     LLVDPQIPYTSHNSSACLSVETNKPDDVWEASQEFLLRESAQGSDAGLALAKWDEVDK
                     CVLSFAGRAKLTVLTMLEAEQTASQSGISLVGLTGTHGGIIGALSAVGLHRAGNDGRF
                     LWLPGLRDLKGKYPVSEIYAKGHVDRVCTLSHVDLHTKTIVDVGEWVRPILREGKATL
                     YVEEINHEWHIIPKEYIKKLSN"
     gene            31271..31846
                     /locus_tag="PL000_09170"
     CDS             31271..31846
                     /locus_tag="PL000_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89112.1"
                     /translation="MNGTSSLKNISKSFPTEWVLPWWEALLLIGGGVTAVVLHRAYDL
                     SLGMPGHHGIEWMALMIIGRASSRFRGAGSLTSIGASFASTLPFLQGENPFTWLFYLL
                     PGPVMDLAYRYLPRYANKLWFMMVLGGLAHATKPIGQLTMNLITDWPFGSFRYGVVFP
                     FVSHFFFGMIGGLLGALIVLGIQRISPKSEK"
BASE COUNT         7843 a         8366 c         7957 g         7542 t
ORIGIN      
        1 tccctttttc tcctcccgct tcgctcaaat tgccgcgttt gttctgctgc tgggtcttgg
       61 gcttggaatc cgcctttacg acctcaccga cctcccactc gattttcatc ccacgcgcca
      121 attgctctcc gcgctcaaag cgcgcggcat gtattaccaa accctcagcg atattcccac
      181 agaacaaaaa agcttcgccg tacagcaatg gaagatccgc gcggcgatcg aacctgagat
      241 cgtcgaacgc atcgtcgcat tcacgtatca attcacgggc gagcagatct gggttgcaaa
      301 agttttctcc tccgcgtttt gggtgatcgg cggactcttt ttgtttttgc tcgcacgtca
      361 actcacctcc accgaaggcg cactggccgc cacagccttt tatcttttta cgccctacgc
      421 cgtcgccgcc agccgcagtt ttcagcccga cccattgatg gtgatgctca ttatcatttt
      481 ttggtgggcg gtttacaaat ggagcgaaga cctcacttct tttcaatggg cggtcattgc
      541 aggtctcttc ggcgggctgg ccatttatgt gaaattcgtc gcggcatttt ttgtcatcgg
      601 cggcgggttg ggatccattc tcagacgaga gtctcttttt gaaacagcac gcaggccgca
      661 gatttatgtc atgaccgttt tgggcatcct gccaggcgcg gcgtatgtca tctatggggt
      721 ctggatcgcg ggttacctcg gtcagcaatt cggcgggcga ttcattccag cgcttttcct
      781 aagcccatcc tattacctcg gctgggtcgg gatgctcaac atcgtcatcg gtggaatcgc
      841 tttgatgctg ggattgctcg gtttgttttt tgtggatgaa aaaaaacgcc gcttctttct
      901 tggactttgg ggcgggtacg ccctcttcgg catgtacttc aactatcaca tttcctcgca
      961 cgactattac agcctgccgt tgattccaat cgtggccctt tcactcgcgc cactggcaga
     1021 atggttcttc gctcaactga cagcattgac gccaacacgt ctcacccgtt tttttgcctt
     1081 cggccttttg ctgctcggca tgttcgcctc cctctggaac atccgcgccg aaatgaaagc
     1141 gaccgactac cgccccgaag cccaaatgtg ggcggagatc ggcgagaccc tcgaagataa
     1201 aaacacagca ggcctcacgc aagattacgg ctcacgcctc gcctattggg gttggaccag
     1261 catcaccacc tggcccacct acggcgacct tatctatcat gatgacctgc gcggcgctca
     1321 actcgaattc gacgggctgt tcgaagacat tgccctcaaa aaagaattat ttctggtaac
     1381 cgatttcgat gaattaaaac gccagccatt actaaaagaa aaattgtctg ggtatcccat
     1441 ctttgccgaa ggcgatggat acatcatctt cgatctcacc tctacaggtt cacaataaat
     1501 gaccaaaaca accaaaccga ctttcgattt catcaccatt gccacgggcc tgggcgcggc
     1561 cttctcggct tttgcgctcg gggtgtatgc ctacctcggc attttctcgc gccatctcgc
     1621 cgatgattat tgcagtgtag acttcatccg caccaatttt ttcagcgcac tgctgaataa
     1681 ctacattttt gtttccgacc gctacaccaa cttcatgctc attgcattga gtgaatacat
     1741 ttcaccgcgc acggtttcga tcctgcctgc cctgttcatc gtcctctggt tgacaggcat
     1801 cgcgtggatg ttatctgaag ccagccgctt taccaaaaat ggctggtcga aactcaccat
     1861 tgtttcgctt acttttttgg tggtgttctt ttccctgctg caggcgccca accgttttca
     1921 aattttgtat tggcgttctg ccatgtccac gcattttgcg ccgctggtct tcatgccgtt
     1981 cctagcggct ttcattttga aggtcatcac cgaatctgag gggaaacccg ccccattttg
     2041 gaaatacctt ctcggcttct ttattgccct catcatcggc ggcttctccg aaccgaccgt
     2101 ggcagtgatg atcaccctgc tggccctcgc cttcgccgcc acctggaaat ggatgtcacc
     2161 atccacccgc agtgccgccc tgaaaatgat cggttggtca ctggttggtg cagtgacggc
     2221 tttgctcatc atggggctgg ctcctgccaa ttattaccgc gtcagcctcg gcaaggaacc
     2281 accgacgctt gccttcctca ttgtccgctc ttttcagtat gggttcaact tcattgataa
     2341 ctccttcagg accctgcccc tgcccaccct cttcaccgtg gccatgccgt tcttcatgtt
     2401 ctacaaccgc tacgcctccc ccaccccagc cctgaccgaa tcccagcgca aactggcccg
     2461 caccctgttg atggtcatac ccgcgctttc gtatttgctc atcgtagcca gttttgctcc
     2521 cagcgtgtat gggcaatcct ttcccgtgga gcgggcacgg tttgcgggcc aggtcagttt
     2581 ggtggcgggg ttaatgatcg aaggcgcctt gcttggaagt ttactcgcgc aatggcggcc
     2641 tgccttcgcc gaaaaacttc cgcttaaact ggcatcggct gttctgtttg cagtggcggc
     2701 gctttatccc ttgcgcgctg gctggctctc cctcaatgac atccccgatt accgcgcccg
     2761 tgccgaagcc tgggatacgc gtgtgattca gatcaatgac ctcatcgccc agggtcaaac
     2821 cgacctgctc atcgttcaac tcgatggcgt ggaaggtgta aaagaattgg aagtcaccga
     2881 gaagcattgg gtgaaccgct gtgctgcaaa atattacgaa gtcaactcca tccgcaccgc
     2941 accgccaaaa tgagcatgaa acgaccgatc cttgtcacag gggcgcaccg cagtggaacc
     3001 acctgggttg gccgcatgtt ggcggcgaac ctgcagaccg cttacatcag cgagccgctc
     3061 aacgtgctgc atcgccgcgg cgtgtatcgc gccgaggttc aacactggta tcaatacatc
     3121 accagcgaaa atgaaggcgc gtacctgcct gccttccatg aactgctgaa ctatcgttat
     3181 cacctttttg cagagatccg ctcgatccgc tcgcaaaagg atttcctgcg catgggccgc
     3241 gacctttcca tttttatgaa aggcaaatta cagaaccagc acgccctgct caaagacccg
     3301 ttcgcggttt tctccgcgcc gtggtttgca aagacattga attgccaggt cgtcatcact
     3361 gtgcggcacc ctgcaggctt tgccagcagc ctcaaacgcc tcaactggcc cttcgatttc
     3421 cgcgacctgc tcaaccagcc aatgctgatg cgtgaccacc tcgaagaaga ccgtgccgaa
     3481 atggaatcaa ccccagcgga tgacatcatc ggacaatcgt ccctgctttg gcggatgatc
     3541 taccgcgcgg ttcacgcttc gcgtacccgc ggcctgatcc ccgaaatcaa aattgtccgc
     3601 cacgaagacc tttcacttga ccccatagca gggtataaat ccctgtatga gtcgctcggc
     3661 ctgacctttg acgaaaacgt gcgcgatacc atcctcaatt ccagcagttc tgaaaaccca
     3721 accgaactct cgaagaagaa ggtccactcg gtgaaattgg acagccgcgc caacatggat
     3781 aactggaaaa aacgcctgac cgccgaagag attctccgaa tccgcaagat gaccgaaagc
     3841 gtctcccacc tcttttattc agaaaatgaa tggtgaccca gtcaccaacg accaaattac
     3901 caatgtccac cctccctctc gtttccatca tcacaccctc ctttaatcag gcacaattcc
     3961 tcgaagccac gattcaatct gtgcttgggc aggaatatcc acggatcgaa tacatcgttg
     4021 tagatggcgg ctccaaagac ggcagcgtgg atgtgatcca gaaatacgcg ggtagactcg
     4081 cctggtgggt cagcgaacag gacaagggtc agaccgatgc gatcaacaag ggtttcaacc
     4141 gtgccacggg cgatatcctc gcatggatca actctgacga cacctacaat ccgcaggcgg
     4201 ttgggcaggc tgtaaaacag cttatggaaa atccagaagt gggactggtg tacgctgact
     4261 gtaactacat tgatgaagca gggaatgtga tcggcaaatt ccctgccgcg caaacggatc
     4321 accgccgcct gcgtgaaggt tacgtccaca ttccgcagca gacaatgttc ttccgcgcaa
     4381 agtattggaa ggaactcggc ccgctcgacc cgtcttttta ttttgcaatg gattatgatc
     4441 tttggacacg aattgcaaag catgcgccgt tcaaatacgt cgcagggcaa acgtgggcga
     4501 actttcgaat ccacaacctc ggcaagacca cctctgccga tgaccgctgc tggcctgaaa
     4561 tgatccgcgt gcattaccgc gatggcggca gttttttttc ggtgatcgtg gcaaagcatt
     4621 atctgcgcaa agtcattggc ccgctgtgga aatggcggat caaaaagcaa gcgaaccgtt
     4681 aagcgagttt ctttcaccct tcccatgaca catcttctca ccccacccac tcttgaagac
     4741 ctcatccgcc tgctgaaggc atggcgcttt tggatgttcg gcgcgcttgt tggcgcgctc
     4801 atcggcgctg cggtttatta tgcagatccg ccgccgtttc gtgcgcaggc caccgtcaat
     4861 gttgatttca atctggaaca agcctggccg caggaaacgg accgtcagca gttttattat
     4921 ctcgagcgcg agtcacgcaa attggaagaa gtcgcctggt ctgatgaagt gttggggcag
     4981 ctatcttctt cattcggcat ttctgtggaa gaactgcgca ggggtaaact gcaactcagc
     5041 cagccagctg aagcgggctg gcatttctac gccgacgatg acgatgcaca gtttgccgca
     5101 tcactggcga ccacctgggc gaaggccttc gtagccaaaa ccgaagcaga tgtggcctca
     5161 ggcgcgatca acgaattcgt ccgccttgaa gcgacccaat cggaaagtac tgccaaagag
     5221 cgcagtattc cattgagcag ttatttactg gcgggttcca tcggttttct tacactcgct
     5281 gtgttcgtat tgttgtttaa acgcaagtga acgcggattc agagatgaga aaaaagattt
     5341 ttatcagcat tcatctgcgt cccaattaat tttttatgaa agaatttttc cccaactctt
     5401 acaaaatctc ccgcattctg tggggtgcgg ctttgttgac cctccccgtg accagtttcc
     5461 gctggtttcc ttttttggga gaaggcacgt tggtccgtcc actggcgatg tacccgctgg
     5521 cggttttgat tccgctcttg ttgattctgg catggcgtgg aaaaacaaag ctcaactggt
     5581 caggcgcgtt gattccgctt ggggtgctgg tcctgttcat ggtggcagcg accagtttcg
     5641 gggcgttgat cgatccgatc ccgttgcgcg ggcaaaccta ttcgggcaga gccatccgtg
     5701 cgctggcgac tctcttcatc ggcatcgcct ttttcatcgc tgccatgtgg atgaacaaag
     5761 atgaagatga tttccgtttc acggtcacgt ggctgtttgc cgggttgtgc ctcgaccttg
     5821 catggagcgg tttgcaggcg gtcacgtttt atacgggcct gctcgaaaaa gaaatggtga
     5881 cgcattggca gcgcgcattt tcaatgcgtg aactcgtgcg cacaaatcga atctccgggc
     5941 ttgcctacga acccgcctgg ctggcaggac aattcgccac aaccttcatc ccgttcctgt
     6001 tcgctgccgt gctgacgaat ttccgcatca cacgcctgaa gtggctggaa cctgtgctat
     6061 tggcattgtc tttgctcgtg gttttggcaa cctactcacg cggcgggttg ctcaccacgc
     6121 ttgccgcagc tgggttgacc ttccttttta cggggcagct tggcatcgtg tggaagtggt
     6181 tcattggcgg cttccgcggg gggatgcggg gaattttatt ccgtgtcggc attctggccg
     6241 caattttcag cgtggcggcg ggtgcgttca tttttctcag ccagaagaat ttcttccgcc
     6301 gtttatggga gacggatgcg caaagcctga gcgaatacat tgtggatatc aatgcgggcg
     6361 cgcgcggcgc ctattcggct ggggcattat ccgcttttga ggagtatccc tgggcgggtg
     6421 tggggctggg ggcaagcggt ttttacattt accagaacct gcccgattgg tcgctgacca
     6481 ccgtgccaga aattgccaaa caactcagcc ctgaaaacaa gttatacccc aaccctaaaa
     6541 atctctatgc ccgcctgttg gcagaaacag gattgatcgg cttcttttta ttcctggcat
     6601 ttcagtttta catacttggc gatatactca gccttctgcg tcacagggaa ggctgggcac
     6661 gttttgcagc agtggcggga atatttgcat ggttggcaat cacattttat aatttcacac
     6721 aggattcgct cacaacgcca aacatctggg tcattccggg catactggtg gggcttgcag
     6781 cgtcttccac aatgccagaa gaacccctgc ctggcattgg actccaatct aaggaaaata
     6841 aatgatcgta ttatctgtcg gtatgccgcg ggctgggtcg ggctggcact acaacttaat
     6901 tcacgacctt atgaaaacca caggctgtgc cgatgcgcgt gatatccgcg aacggtatcg
     6961 gcttcaaaat gtcttgaccg aggtcaattg caacatcggt gtgctttcag ccagaagact
     7021 tggactggtg atgatccctg cgctggtaaa tacttttgtc atcaaggctc attccgcgcc
     7081 gacgaatgct tcgcgtctgc tttcgaccat gggcaggctg cgcatcacgt acatttatcg
     7141 tgacccgcgc gatgccatgc tctccgctta tgatttcggt cagcgggcgc ttaccaaggg
     7201 ccgccccaat gccttctcgc atctttcaag cttcgagaag agcgtggatt ttatgatgga
     7261 atatgtccat atttgggaaa aatggattaa agaaaagaac gtgctcaccg cacgctacga
     7321 agacctgctg atgaattacg atgaggaatc aaccagactg gtggaatacc tcaaattgaa
     7381 cggcagcagg cctgaggtcc gcaaggtgat cgagcaatat cgtccgggca cagcggaagg
     7441 tcagcagggc ttgcattttt ataagggcaa gatcggccgc ttccgtgatg cgtacaacga
     7501 ggaacaaaaa tccatcttaa aagagaaact tggcccatat ttgactcaaa tgggctacaa
     7561 tgaatgacaa ggtgtaatct atttgtaagt cacccaagtt gtcgcgcgga atgtttttcg
     7621 ttatcatagg gaagttatgt tacgaagatt tgattcgccc tacatggcag actggtttgc
     7681 catatcattg agatggatca ttctggtcgg ctttgttgtt tcacttggat tgggggacaa
     7741 acttgaaatt gcagtttcgt ggccgctggg gttattgatt ctctggaacc tgttcatgac
     7801 tgcactggca agcatgaaca tccgtgttgc ctatcaccgt ctcttcagca ttcttgtcga
     7861 tatcacactg gctgggttgt tcttttggac ccagggcggc ctgcgcggac ctgccgcatg
     7921 ggcgggcatt ttacccattc tgacggggtc gatatttttt gagttgttgg gcgccgtagc
     7981 cgcctctgtc ttcttctccg ggctcatgat ctatctgggg ctgcaagacg gggacagtct
     8041 gccgctttcc atcacattct cagctgcact gatcgcatta agcatggtgt ttggttttct
     8101 cggcaaacgc atgatcattc acatgcgcaa gaaccgcgaa ctgtggctgg attcagaaga
     8161 acgaaaaagc gccatgcaag ccgagcggat gcgcgccatt tacgaattaa ccgccgcatt
     8221 atcctccacc ctgagctata aacgcgtgct cgaatccgcg ctgaatttga gcgcctcggc
     8281 gctaaacccc gaccccgaac aacttcacag cgatccgctt gtcggtgtgg tgatgctgtt
     8341 caatggcggc aaactgcgca tcgggtcggc acggcgcttc accaccgccg acatgcgtgt
     8401 gaccttcgat gcggcggaag gcattctcaa aaaaacaatt gacgaaggtg aaccgctctg
     8461 gttcaaagac attggctacg accccgaact gggccgtgtg atcgcgctcc gcaattgcac
     8521 cagcggatat tgctttccgc tccgcagcgg cttcaacgtt tatggcgttc tgctgtttgc
     8581 ccaccccgat gcagagtact tcaccaccga gcgccgcaac ctgctcgata tcatcggccg
     8641 tcaggccgtc atcgccattc aaaatgcgcg tttgtatcag gaccttgttg aagaaaaaga
     8701 acgcatggtg gagatccatg aagaagcacg caaaaaactg gcgcgagacc tgcacgacgg
     8761 ccccacccaa tctgtagcgg ctattgccat gcgtctcaac atcctgcgca gaatgatggc
     8821 aaaagacgtc gcatccgccg gggatgaagt ggtcaaactg gaagaacttg cccaccgcac
     8881 caccaaggaa atccgccaca tgcttttcac cttgcgcccg ctcatcctcg agtcgcaggg
     8941 attggtcgcc gccattcaag ccatggccga taaaatggct gaaacctatt cacaaaaagt
     9001 gacccacaac attgacgaac gggtcacctc gcaattggaa atgggcaaac aaggcgtcat
     9061 cttctacatt attgaagaag ccgtgaacaa tgcccgcaaa cacgcagcgg cagagaccat
     9121 cgcggtaaac ctcacacaaa tggatgcagg cattgccctg cttgaaatcg ccgacaacgg
     9181 cctgggcttc gacgtaaaat cggtcactga agcatacgac aaacgcgcaa acagcagcct
     9241 cggcatggta aacctgcgcg aacgcgcaga actcgtgaat ggcttgttcc agatcgactc
     9301 cgcgcccggg aagggtacga aggtacaggt gtacatcccg ctcacagagg aagccgcaga
     9361 ccgtttacat tacatgcgca acaaataaaa aatgcccatc gcagcaaacc tttattattt
     9421 ttcccacgac acagaagacg acctcgaaac caaacgcccg cccatcattc tcattcatgg
     9481 ggcgggcgga aatcttttgt cctggccgcc acaggtccgc cgcatggtgg atgaaagggt
     9541 gtactcactc gacctgcccg gtcatggcaa atcggaaggc acaggcaggc aatccattga
     9601 cgagtacgtt gaagatgtac tggcatttat gcaggccttg aaacttcgca cggccatctt
     9661 cgcgggcgtt tccatgggcg cagccgttgc cctcgcactc acactaaaac acccacgcaa
     9721 agtgcgcgga ctttgcctgc tgggcagcgg ctccaaaatg cgtgtggctc cccttatttt
     9781 ggaaacagcg ggcaacccca acaccttcga atctgctgtg gatttgatca atgaaaactg
     9841 cttcagttcc gaagccccgc aacacctcaa ggatatttca aaacgccacc tgcttgatgt
     9901 gcgcccgccc gttttgctcg gcgatttcct cgcctgtcat gaattcgatg tcaccagcca
     9961 gctcaaaaaa atccgcaccc ccaccctgct catttgcggc gccgcagaca aaatgacccc
    10021 cgtccgctat tccgaattga tgcaggaaac aatgcccaac gcaaaacttc acatcgtgga
    10081 gaatgcaggg cacctcgtca tgagtgaaca acccgatgtc gtagcagacc tgatgaaaca
    10141 gttcgtcgat tcaattccgg tccgcaccag gaagacgaaa aaagaccgcg tcgaacttaa
    10201 cgttgaatct gatcctgccc aggaaacaga atcagcgctt caggcagatt ctgtcccttc
    10261 gacggagccc aacagctaag aaaatatttc cttcaaaaca ggcattgcat taataaccgc
    10321 cctggcccga tggctgaggc ggtttttttc atccatctcc agttcagaca tggttttgcc
    10381 caattcaggc atgaagaaaa tgggatcata cccaaaaccg cctgtgccgc gttcctcggg
    10441 gatgatttcg ccgtggcaat tgccgtcagc gatcttcaca tcgccgcctg gaatggcaat
    10501 ggcaatcgtg gcatggaagt gtgccttcca cggacgcggt ttaccttgca ggttttcgag
    10561 catgtaggct cggcggtcag cgtcatttgc gcctggcttg gggtggtaac gggcagaata
    10621 caacccgggt gcgccgtcga gcgcatccac ttccaggcct gagtcatcgg caagggagat
    10681 caatccgctg gcctgggcga aggcgatggc ttttttggct gcgttttcaa tgtaggttgc
    10741 cccatcttcc tccacatcga gatccaggtt gatctcagca ggggtgacga gttggacatt
    10801 caattctctg agaagttcct gaagttcttt tactttgcct ttgttgttgg tggcgataag
    10861 aagttttttc attttgaatt cctgtggcgc aagataatat cttgcgttac atgtttcttt
    10921 gcccattgtg catgttcgcg gatcatgggt tcttcatctt gagcggcttg ttctaaaatt
    10981 ggaaggtcgt tttcactgcc cgtattgcca acagcaaccg ctacattgcg gagataccca
    11041 cgtcgcttgg cacgtttgac ggggcttttc ttgaagcgct gattaaattc aacggatgtc
    11101 agcagcaggt ctgaggtcaa gacaggaagc gggattttcg attcgaaggc ggggtctgcc
    11161 tgaggtgaaa accgattcca tgggcaggct tgttgacagg cgtcacatcc gaaaatccaa
    11221 ttctgcatga gtggacgtaa ctcttcgggg atgtcctctt tgagttcgat ggtgaggtag
    11281 gagatacagc ggcttgcatc gagggtacgg ttggggagga tgcattgtgt ggggcaggca
    11341 tcgaggcagc gtgtgcaggt accgcaatgg tcggtgatga gggcatcgtc gggttccagt
    11401 tcgataccaa gcaggatctc ggcaaggaaa aatgcagagc ccatctgcgg gttgatgagc
    11461 atggagtttt ttccgatcca gccaaggcct gcgcgttggg caagttcacg ttcgaggatg
    11521 ggacctgtat cggtgtaata acgatttgga atgggatgcc cgagttgttc ttcgatgaaa
    11581 tccacgatct gctgcaggcg cggagggatg atatcgtgat agtcatcgcc gagagcatag
    11641 gatgaaatgc gaaacgcttc gcgtgaagat tgagggttga aggttacagg ttggtatggc
    11701 agggcaagga cgagaatgga tcggcactcg gggagaattt gtttggggtc ggcacggcga
    11761 atgcggctgc gttcttcggc gaggtattgc atgttgccgt gtttgttttg atgaagccat
    11821 tcttcgaaca tgccgtagtg tgcaggcggc tcgcaagaag taacgccagc caggatgaat
    11881 cccagctggc gcgctttgtc tttgatggct tgcttgaggt cggacaagtt aattcgggaa
    11941 aggtgtgtcg cactcaatgt agaatcgtta tttgacgacg atcttcacaa agatggcttt
    12001 tccaaacgta acgcccttgg catttgccat ctgccatgca ctggtgtatt caccgatctg
    12061 gtcgggagcc ttgaagttga cggatacttc cacttcctgc cccacttcaa caaggccaat
    12121 gggaacgggt acgccgctca tcttgtcacc ataggtgtaa accaagccgt aaccatctcc
    12181 ccaggcacag atccctgtat ttttgatctt ccatgttttg acaaattcct gtccgggggt
    12241 catggcggtg ccatccagga tggtgacatc cacagtagcg acgtcaaatg aatagtcgtc
    12301 gcataatggc cctccaggag tacccaatgc gatctgggtt ggatctgttg caaatgcaat
    12361 ggttggggtt aatgtaggcg gttcagcagt gaaggtgggg gtgggaggca ttggggtggg
    12421 tgtgaacgca gcagccgtca gtgtgaattc ggcgactacg gtcatggcgg cagaagtgcg
    12481 gacggccagt acatcgggcg tcggcacgat gggggtggca ggggcacagg cggccagcat
    12541 cagggtggcg attattgcag aaatgacaaa tctagctttc atggttcctc tcttgatgta
    12601 taagaacaat tacttgatca cgtacttaac ccagaccatg gagccgaagt aattgccctc
    12661 atcatctcta agtttccaag taccaatgta ttcaccaggg gcgttatttg tcaccagctt
    12721 caagacaaac gaaatattgg cgcccggttt cacataatct tccgctttct tgatgacaat
    12781 atcggagcct ttaaagcccg gagtggaaaa atcaggaagg aaggcaaagg tatagccttc
    12841 gtcccacaca caagaaccgg tgttcaaaaa ttggaagacc ttttcaaaag ccttgccgcg
    12901 ctgcaataca tcaccatcga aaggctcgct ctccttgacc aaaaccccgt cattgcagcc
    12961 attcttggtg gtgatggtgg caaaaacagc ggctgttggg atgggcgagg ccagcggggt
    13021 aatacccaga acaggctgct gagtgttgaa gggaattgga gtggcatcac cgccgccgac
    13081 gggcgcaaat gtgggcaaag ccgcaagagt actagttggc agaagggtgt tggtcggcaa
    13141 aggagttggg gagctttgtg ccgccacctg ggtcaaaaca gcgttaaagg cttctgtctg
    13201 aactgctgcc agatcctgtg ttgggactgg ggtagcgccc agattacagg aacttaacac
    13261 catggcaagc accaaaaaag acgccagcca aagaaattta cgaactctca ttatagtaac
    13321 ctccggacat agttaactca aagttctgcc caactccacg atttttgaag attttgaagc
    13381 aaaattgcga attcggcgag gcagaaactt tgattcgtta acttgattat aacgcaccta
    13441 tgaaagcgtt tcttatggag tatcgcataa attgggcatg cagattttcc gtttttcaca
    13501 aaaagggaga aaattccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa
    13621 acaggagaaa ctcaaattat caaggtgtgg gagtggcggt cgcagtaaag gttggcgttg
    13681 gagtgaaggt aggtgtaaat gtcagtgtag ggatggttgt gggaaccggt gtaccctgcc
    13741 gcaaaatgaa gggatgcagc cagatggcat cgtcctgatt ctgtccgccg tgagcggtga
    13801 ctacaaggat gaacttcact gtttcaccgg ccagagggct caagtcaaaa tcgatcgggt
    13861 aggaattccc ttcatacact tcattccagg taaacaggtt tttcactgtg ccgttatttc
    13921 ggtaatcaag acgaaagacc acatcacacc gcctcgaacc atactggcaa cccacaatcg
    13981 tccgaaaatg atcgccttcc ttcacggtaa aggcaggata ctgtccacta atttcacctt
    14041 gacgcgcatc ctgtggaaca gtgagcaggc tggggttatt ctcggttacg ccgttttcca
    14101 tcatgggggt atccaacctg acaacaaaac catttgcatc acccctcgta cccgggcacg
    14161 gcagaggagc cccctcgttg cgccactcgg cttcacaaaa atgctccaca aagctataag
    14221 ccatgtagct ggcgccaggc accctgatct caacccagaa tgccttttgg gctttgtcgc
    14281 caatcccaaa attaacacca ttggcattgc gtaatttcca atagccaatg tattcccctt
    14341 ccgcgctggg ggcggtcaac tgaaccgaga tgtcaattgt gctgccaggg cttacactct
    14401 tcgaaagagc tgcggtggtt gggccgctca ttgcataccc gccatcaaac acaagcgaat
    14461 aagccgtcgt ccacgtgcag gtgccaatat ttttaatacg ccaggtcttt tcgaaggaag
    14521 tattccgcac cacaggactt ccatccggat aagttatatc tttgacgaat tccgccgcat
    14581 cacatctgga caccggagct gctgtggcat aaatgggctg attcgtggct gtggcagcag
    14641 gcaacccagt cggaggcgga gcagggctat atgtaggcaa tggcaatcca ggggttgcac
    14701 tcgctcctac agcggcggtc gaaacctgtt gtgtagtgcc agcttcaagg gtctgagcag
    14761 aagcggtgta aagtccattt aatgtggcaa atgtatctgg ggtcgctgtg ctgtttccaa
    14821 gattacaggc caactgggcc atcatcaaaa acgtaacaaa cagggaaatc ggggcttttt
    14881 tcatgaacat gactattctc cggaagatga atttccaggg attaagacgc ctgtgaaaga
    14941 taattggttc cgtccattta ataaagaagc cagcttgcgc tggcttccaa atgtttatga
    15001 gggaaaaccg taagatcagg aacttccgca caaataggtg aattcagcat gcggatactc
    15061 ctgctccacc gggtccacaa tcacaatggc catccatttt gtgcccggcg tgctgccaag
    15121 gtgaagcttc cactcgcgcg aaatggtcag cgtccctgcg gcatccatct ttaatttctt
    15181 gggattggaa ttgttattgt cactttgcat ccagtaatag gtgaattcga tgggaccatt
    15241 gctggtaaac gtggcataca caatatgatt cacattggca gggcaccctg tcgctgggtc
    15301 acgtaccaca ttgtattcta cagaagtaat ggcaaacccc gggttggctg aagaggatac
    15361 gacgatctgg gtatagaacg gggcgctgta ctcacccact ccaaactcaa ccccatccgg
    15421 agtctgtaac atccacttgg aggtgtaggc ctcatcagct tgtggggctg tgaacagaat
    15481 tgaaatggga acgatctgtc cggggtctac tgcctgcggc aggttgtata cataggcacc
    15541 accaagcaca ttgccatccc aaaaaatgat cttataactg gtatcccatg agcatggact
    15601 ggtattcttg atctcccaag tcttggtaaa accctgcccc ggtttgaaga tggttccatc
    15661 tggaatgttc tcactaacca ggctggcctg cgcgcattct gaaacagacg ggttacgcgt
    15721 ccgtgtgggc gaggctactg gcgcaagcgg ggtaaaagtt ggggcaaaca acaaaggggt
    15781 cggtgtaaaa gcgggggctt caggagtggg gttgcttaca ttgccaagcg ccgccacggt
    15841 ttcagcgact gcggtttgta tgctgttggt atcctgctgt gtggtttcgg gggtttctgc
    15901 actgccgcta caggctgtaa tgagcagaga caagacaacg atcaaggtaa aaagtaaata
    15961 cttgaatttt ttgaacattt ttttcctttc gcacagataa ttatactgtt aaacagaagc
    16021 gcccggattt cccgggcgct ttacgaaaat cttttaaaag gggttatgga caggtaaaag
    16081 ttgcgcgccc aaactgtttc tggttcggtg agtcgatgta aacatcaacc cacgcgctgc
    16141 tgcccggtgt attgaaggtc atgggaatgc tcgggctggt ttgcgtacct gcagaattga
    16201 acgtcaggct ggcaggatag gggtctgtgg agccatcact gaaaacgcgg tgcagggtga
    16261 cggtccccgg accgttggta gtcacactaa acgtatagtg gaaattggga catgaacctg
    16321 tgacattgaa caagacactg gtcaccgtga acacaaaggg ggttgaagta acagtggctg
    16381 tgggtgtaat ggtcgttgta acgcttggcg agccggtagt ggctgcctgc actttaacat
    16441 ccacgtagaa agacttccct tgatatccct ttacaacggg cacgaggaca ttgccattgg
    16501 taacgatacg ccaatatccc ttgtattgcc ccaaagtggc aggagcagtg agattcactt
    16561 caaggtcaat ttcctggcct ggcgccacag tgctgatggg ttttgaggca gggccaccca
    16621 ttgagtcgcc gctatcaaag accattgaga aaccagtcca ggtacaagag ccgatgttct
    16681 tgatccgcca ttttttgaca aaagcctgat tgggggtcac cacagttcca tcagggatgg
    16741 tgacatccgc aacgaactca gccacattgc agttggaagt tgccgtagcg atcggtgtat
    16801 tcgtcaaaac gggaagagga atgggggtaa gggttgcagt tgtaaccggg ggagccaccg
    16861 taaaggtggg agtattgttt atgataattg caggggtcgc gctcaataag gcttctacag
    16921 tttgggcagc cgaggtactt gctacatctt ccgcattgtt tgagggcaaa ttacatgcgc
    16981 tcaaaaacaa ggtgaagatc atcagcagat taaataagcg gatttgtttc ttcatgattt
    17041 tctcctttgt agtcgtgata ttgtaccgca ggtcaaattt cgagaatttt gcataaattt
    17101 agacatcctg ctgccaccat caagttttta gccgtgaatt aagcgaatgt tctaaactcc
    17161 attcatcata cgcggatcaa gtgacagctc cgattgtctt ataaaaatgt atgcagacct
    17221 ggcgggccac ttgcattaac gcctataagc tttaatttgt tcccaagaca aattttgaat
    17281 tcagacaaag aatgcgtagt ttgcaaggaa acaaaaaaat agtgcaaaat cttgtgattt
    17341 tgcactattt tgcttatgcg caggttttta ttggccttga acctgaagga tgtttaccac
    17401 cccattcaat gggtagtacg caatcaagat cttgtttttc ccatgtgtga acaaagccac
    17461 agtacacaaa tagcctttct gtgtcttcac ttcctccgtt ttttcaagga cccagccatt
    17521 gtttttggcc tgctgatcat aataggcaaa aacggtatcc caggtagttg tagctggcac
    17581 agccgacatc aattgcaagt caccgctgat gcctgattgt tcgcgcagga tggcaacaaa
    17641 atagttcaca tcatcttgag gttcggcttc acccggcatt ggagggaaca tgaaaccatc
    17701 cattggatcg ggcgcgccac catcagtaga gtcggcaggg gcatccacta ccgcaccggg
    17761 atcagcaggg gcgccgctat cagcaggagc accagagtca acgggggcgc cagaatcggc
    17821 ggaggcttct gtaggagcgg ggttcccgcc accacaggct gatatgctga caaccagcac
    17881 aaccaaaatc agcatatata tgtatttctt cattttttat ctccttgata tttggaacat
    17941 tacaaaacca cttccctgct catcttcaac aggataccgg caagccaaaa gacagccagt
    18001 cctgttttcg tttgaaacgc agggaagtga tattttacaa caatctgttt tttacgctgg
    18061 cgaagattcc acgtgagcgc cagccatcca tagaccaagc tgttcacgag tggttttctt
    18121 tgcttccact actgtcacaa tttgtccacg atacatgaca gcgatgcggt ctgaaagcgc
    18181 catgatctcg tccaactcag cggaaataag aaggacagcc accccgcggt cacgcatctt
    18241 gatgatctcg ctgtggatat attcaatgga accgacgtcc agaccacggg tgggttgatt
    18301 cgcaataacg agcttcacat tgctgcgact taattcacgc gccacaatca ccttttgctg
    18361 gttgccacca gaaagtttgc cagcgctgac aaagggagat ggtgtgcgca catcaaatgc
    18421 accaataagt ttgcgggcat tctcatccag cgccttttgc tggagtacac cacgattgct
    18481 gaagggtggt cgataataat cgcacaaggc catattatcg gcaatggaat aggagagaac
    18541 aagcccgtgg cgctgacgat cttcggggat atgagccagc ccggcttcgg tcatgatccg
    18601 tggcggtttg ccggtcaggt caatgccatc catggtcact tttccgcctg tcggagaacg
    18661 caacccggtc aatgcttctg aaagttcagt ttgaccattg ccttgaaccc cggcgatgcc
    18721 gagcacttca ccaccgcgta ccgtgaatga aacgttgtgg actgtttcca ggttacgctg
    18781 gtcgcggacc gagaggcctt ccactttgag gatatcgcca ctggcggcgt ggtcatcttt
    18841 ttcaacggtg agaatcacct ggcgaccgac catcatattg gcaagctgtg cttcgttcgt
    18901 tccctgaggc tgaaccgtgt tgatgacccg tcccgcacgc atgacggtga tacgatctgc
    18961 aacagcaagg acttccttga gtttgtgtgt gatgaagatg atggaaacac cgcgtgccgt
    19021 caggtctcgc atgattctga agaggtcttc agcttcctgt ggagtgagca cggctgtagg
    19081 ctcatcgagg atgacgatct ctgcattgcg atacagggtt ttgacgattt caacacgttg
    19141 ctgcaaacca accggcaggt cacccaccaa tgcatttggg tccacatcaa ggccatattg
    19201 tttggatatt tcattgatac gggtggcaac agctgcacga tccaacgaac cgcgtttgac
    19261 ggtctcatca cccagcatga cattttcagc cacggtaaat actggaatga gcataaaatg
    19321 ctggtgaacc atgccaatgc ctgctgcaat tgaatcgttc ggggaatcga tttcgatcgt
    19381 ttttccattg acagcgatct cgccagagtc ggggcgatgc aggccataga tgagattcat
    19441 caacgtgctt tttcccgcac cattctcacc caacaaggca tggatctcgc ccttacgcag
    19501 gtcaaaattg acattatcgc tggcaagcac tccggggaac tgtttggtga tgttcctggc
    19561 ttcgagaacg attttggctt ctgtcatttc cgcctcctat ttctcgtatg gaacgccatc
    19621 tgcagcgggc ggcgtggtct tgccgatgat acccgtcaga atgaacatgg taaggatgta
    19681 tggagtaagg cccacaatat aggattcctg aaggaaagac cattggggtg ggatcacatc
    19741 acggaagatc tgcatattaa tgagcagcgc ctgcgatgca ccgaaaacca gcgaagcagc
    19801 ccatgcgccg atgggcgtcc agtttccaaa gatcatcgcg gctagtgaaa taaatccgcg
    19861 cccattggtg agcaaaggct caaaggaggg aaccgactcg atggtaaagt acgcacctgc
    19921 cagaccagca aacatgccgc cgatcagaac atttgcataa cgcatcttaa tcacattgat
    19981 accgacggta tcagccgctt tgggatgttc acccacggca cgagtgcgca aaccccagcg
    20041 ggtattgaac agcacgtaat gtgaaaaaaa cacaagcaaa atggcgctaa tggcaatcgg
    20101 cttttgatca aaaacgcggt tgatcgctgc gagattttca aggcaggaag cagaagaacc
    20161 gcaaacagat gtaaatatct cagtaagcgg aacgtggatg gtgggaagga cgcccgggga
    20221 attcggcaca gaaccgccaa aaacactgcc ttttccaaag aacaattgac gattcaaaaa
    20281 gccagtcaac ccaacagcca ggatattgat aacagtgcca ccgatgatct gatccacttt
    20341 gaaggtgatg gaaaggaccg cgtgcagcag agacatcaac accccggtca aaatcgcaaa
    20401 aaacacaccc atgccaatgc taaaaccaac tccaaaacca aagacgtggt acatgtaaat
    20461 ggcacccagc catccaaaaa acgcagaggt caacatcata ccttcaatgc cgatattgac
    20521 caccccggcc cgttcacaat agaccccgga aagagcgccc agtgccagtg gggtggcaac
    20581 tgcaatcgtg gtagccaaca tactggtaat gatcaataca ggcggttgtt ttacctgtcc
    20641 gatcatgacg actgtgccaa aaagaacaac aatgatcagg gcaataaagc caaaacgttt
    20701 ccagtccatt ttttctccta gcctccccaa ccgcgggtga gtacgacacg ttcacctttg
    20761 gatttgatgc gatagatata tcgcacgatc gcatctgctg caacaaagag caggatgaaa
    20821 gcctgaatca tggagatcag atccacaggc atctgggtga ggaactgcat tcgtgtcgct
    20881 ccattacgca tcgcagcaaa caggaaagct gccagcacca cccccaaagg atgtgatttt
    20941 ccaagcagag cgatcgcaat ggcgtcataa ccatagcctt gtgaaaagcc aagttcatga
    21001 cggtaattca aaccagtcac ctcgatcgtg cccgccagac cagccaacat accagaaagc
    21061 gccatcgtaa ggatgacgat ccgcttcaca ttgatgcccg catacttcgc cgcatccgga
    21121 ttcaacccca cagtgcggat ctgaaaaccg agggtcgttt tgtttaacag ccaccaaatc
    21181 ccaaatgcaa ccagcaaggc cagaatgaaa ccccaatgca cgcgcaagcc atcaaaaatg
    21241 ggagggatgc gcgcactatc cgcgataagc ggagtgcgtg caattacatt cgttgggctg
    21301 cgatctttca tcacaccgtt caacaaatag gaggttgtat tcaacgcgat atagttcatc
    21361 atgatggtgt tgatgacttc atgtccgcct gtataggctt tcaaccagcc gacaatagcc
    21421 gcccacaggc cgcccatcaa cacaccgaaa acaatggcca gcggcagatg aataatggca
    21481 ggcaggtcat acccaagcca ctcaggcagg gcatagccaa ccaatgacga ggcaaccgct
    21541 ccaacagcca gttgaccttc tgcaccgata ttgaacaacc cacccttgaa ggccagcgca
    21601 acagccagcc ccgcaaagat gtatggtgtt gcccaaacag cggtttcact taatgccttc
    21661 gttgaaccaa atgagccttc aagcaaaccc cgataggcca gaattgggtt tccgcctaca
    21721 aacgcgataa tcacgccgcc aacaactacc gccgtcagaa tcgccaaaaa tgggaccagg
    21781 ctgttatctg aaatcgtctg caatatttgt tttatcagcg aatttgattg cttttctgat
    21841 tgcatccttc gctccagttg aactgtgaat tgtaggacaa ttatatcaaa caagccgtct
    21901 ggaaccccat tatcggtatt acaaaatcaa ataaaaaagg gaaagagact cgcgcctctt
    21961 tcccttttct tacttcgtca aaaaattacg gcttcacagg agcaacattg gtcttgatgg
    22021 aaccatcgag cagacccttg ttgatctctt ccatcatggt cttgacttca gcggggacgc
    22081 tgctgtcgag atcgtggaac ggagcgtaac cagcggcacc gaagacgtta ccatcagcaa
    22141 tggagccttc tttggtggcc ttgatcaggt cggcaactcc gggggtgatg agcttcatcg
    22201 cgctggagag catgcgcgga gcagcttcgg gcagggtcag gtactggtcg gtgtccacgc
    22261 cgatggcgta ggcgccagcc tgagcggcgg cggtgatggc gccgttaccg gtcaaaccac
    22321 cgcaaccaaa cacggcatca gcaccctgat ccatcatgga tttggcggtc tgagcgcccc
    22381 actcgggatc ggtaaaggtc ttgtcgaagc ccacatcgct gtggtacaca acgaacacct
    22441 cagttgtggt gccgttcttc tcatcggcat acgcagcacc agccttgtag ccttcaccaa
    22501 agcgccaaac ggggggaacc acatcggtac cgcaaacggc gccgatcttg tggctctcag
    22561 acatcatcgc ggcaagggcg ccaacgagga agccagcgtt atcttcaggg aagttcaaac
    22621 cagaaacgcc cgcaacggtc tcagcctgga actgatccac accgatgaag cgaacattcg
    22681 ggtaggtggc agcggcagca gcggtggctt caccgagacc gaaaccgacg gtcacgatga
    22741 catcgtagcc ggcatcaccg aactgagcga tgttcttggc gtaatccttg gcatcagacg
    22801 tttcgatgaa ttgaacaaca tcggctacgc catcagcctg agacttctga acgccttccc
    22861 aggcagactg attgaaggac ttgtcattga tcttgcccac atcggtcacg agacccacgc
    22921 agaacacttc ttccttcgag cagtcttcag cggtcggagc acttgcaccg caggcagtga
    22981 ggaccatcga agcgacgatc aacaaagcca ttacaaagta aagctttttc attttttctt
    23041 ctcctaaaag aatagggttt taaaggtctg ccatttctgg caaaccttta cgacagaaca
    23101 agtataaatc gttatatttc attgtgcaag cctgaactat tacaagcata tttcaggcaa
    23161 aatcacggtt tttcgcggga tacccctgtc tgaatttcac catttaaaag ggcagaaagc
    23221 agggaatcca actcacgcgt caaaccttcg gaaaattttt cattaagcgg cacataccca
    23281 aacgaaccga ttcccgcctc ggaaagattt tctccctgaa tcatgcgcat cacctcctgg
    23341 acctcgaaac tggcactgcc ataaacagaa gtgaccacac cgtaaccttc tgcactctgg
    23401 tcgcgttccg cgccgatcgc cagcacctgc ccatccatcg ctgcacgaag cgccccctcc
    23461 cctgtcgccc cacccgcagc gaagatcaca tccgccccgc gttgaattaa agactggcct
    23521 gtttcgtacc cccaaacctc atccagaaaa aggtcatcac ggctggcgtc atcgcggtaa
    23581 aacacaagca ccttgatgtt ttcatccacc gactttaccc ccgcgttgaa tccctcacag
    23641 tagcgccaca tcgcgtcaat gtcgctggtt tcgcaaactg cccccaccac ccccgcacca
    23701 gacagccggg ccgccaacgc cccggcgaga aaacccatct gatcttcggc aaaattgaca
    23761 gggatgaggt tcttgcgtgc ctctttttgg ggttgggaaa tgcccacgaa aacagaatca
    23821 ggatacaggt ccgcactacg aagcgtttca tcatccatac cggggccgat ggtgaaaatc
    23881 acatcgaccc cctgttcagc aaaaaacacg atattcttct cataatcgcg ggtatcaact
    23941 gattcgatat attccaccct gtcactcaac ccgccagatt gagcctcctg cagccccgcc
    24001 caggtttcct gattcatacc gtgatcttcg aggccgcgtg tatcagtgac cagagccgcg
    24061 caaaagacct cttcactaaa acaatcttgc gattgcatgc aagccgtgag agctgacatg
    24121 gcaatcgcaa gaagaaatat ttttgatctc aattccaaat tcctgatctc taaactgcct
    24181 gcgccgcttc tccgcgtttg acgccaagca tcaaccacaa ggcgatcatc atgaaaaacg
    24241 gtgcgatcag cataatgatg ttgtagtttc cattggtcaa ttgaatggcc cagccattca
    24301 cattcgggcc aacgatggca gagaaagtgg aaaacagata atacaaccct gtaaatgtgc
    24361 cgatgcgcgc cgcgtcagtc aggtctacga tcatgggcag ggagttgatg ttgatcaaag
    24421 cccaggccgc accgccaaaa atgagcagaa cgcccgccag agtcaacatg cgtggaccgc
    24481 cttcttcaag cggaatgcca accaaaggca gcggagagat gcccgtcagc agggctttgg
    24541 cgggggtgat atagagcaaa accagcataa tggtgagggt gatcaaaccg atcgagatgg
    24601 tggtgcggcg ccccaacctg cccgcgataa aaccagatgg gatcgcaaac aggacaaaga
    24661 acaaaggcag caccgaaagc agggttgccc cgtcaccttc attcaacccg aggtgattct
    24721 tggcataaag ggtgaaaaag gtttccactg ctgaaaaacc aatgaaccag aaaaatatgg
    24781 caaaaagaat gcggagaccg cttttatctt cactgctgac aacttcgcgc agggaagcaa
    24841 gcatgcccgg ctgttcttca cttgccgcat actcttttgg ctcttcgata aaaagataaa
    24901 cgatcaatgc cgagagcaaa accaacccgg agccaagcca gaacggaaaa ttcgggctta
    24961 acttgtacag caggccgccc gtctgcaaag cgacaatggt accgatgccg cccatgaaat
    25021 tgatgatgcc attggcctga gagcggaatt cagaaggggt gatatcgggc atcaaggcga
    25081 tgacaggtgt tcgccagaat gccgcgctca acagcagggt acttgtgcaa gccacaaaca
    25141 acggcaatat ggcaggcaat gggatcaagc caaaggctac agccgtgatc ggtgcgccga
    25201 tcagcaaaaa tgggatgcgg cggcccaggg gtgtccgcaa tcggtcagac catgcgccga
    25261 cagggggctg aataaaaagc gcggcaatat tatccagcgt catgaaaaag ccaatgacca
    25321 ccggagacag gccaaatttt tctgaaagaa agatcggaac aaaggcgttg tacacacccc
    25381 aaactacgct taaaccaaaa aagccaaagc cgagcaaaaa ggtcttaccg taattcaatc
    25441 ttgatgacat cgtttcctcc atgcagtgga tatgggatgg ggttcagatt gcnnnnnnnn
    25501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcttcttt tgtcaattcc gctttttcaa
    25621 gcatgtcggg gcgttttttg aaggttcttt ccagagcctg ttcgcgccgc cacttgtcaa
    25681 ttttggcatg gtcaccggaa agcaagatat cgggtgtacg ccagcctcga aattcaggcg
    25741 gacgggtgta atgtggatac tcaagcaggc ccatggcatg tgagtcatcc tgtgcgccgg
    25801 tagggtcgcc aagcacatca ggcaacagac gggaaaccgc atctatcaac atgagtgcgg
    25861 gcaattcgcc gcctgtcaac acaaaatcgc cgatggagat ctcgtcagta accaaatgct
    25921 cgcggatgcg ttcatcaaaa ccttcgtaac ggccacagat cagggcgatg tgagagtatg
    25981 cggaaagctc ctgggcaacc gcctgattga acaaacgtcc ctgcggggtc agcaaaatga
    26041 ccgggatggg ggaagaatcg ttctcattgg atattcccag cacagattcc accgcttcga
    26101 aaacaggttc gggtttcatt accatcccac cgccgccgcc gtagggggta tcgtcggtca
    26161 catggtgttt gtcgtgggta taatcgcgga tgttgtgtac ccgcacatcg atcaatcccc
    26221 gttcacgggc gcgtttgaga atactggtat caagatagga agaaaaaact tcgggcaaaa
    26281 gggtgaacac ctcaaattgc atgcggcgat tttagcatag aaaccttgca ggacatcctt
    26341 ttagccatcc acgggtttta gagagtgtcg cactaccaat ccaaaccatt cggagtttca
    26401 agtgaataat tatattgccc tcgtcatcac ctttgccgcc tctctattgt ttctacgatc
    26461 catggatttc attgcccatc gcggctggat ggacggaaaa ctcagtcgta agatcatcca
    26521 cattggcaca ggtccgcttt ttgtgttgtg ctggttcttt ttcaacgatg cacccgaagc
    26581 acgctggctt gcggcgctgg tgccttttgc tgtgacggca caattcgccc tgatcgggct
    26641 gggagtcatt aaagacgagg cctctgtgca ggccatgtcg cgcacaggtg acccaaagga
    26701 aattctgcgc ggaccgttgt attacggcat catgttcgtt gtattgacgc tggtgtattg
    26761 gcgggagaac cccaccggca tcatcgcgtt gatgatgatg tgcggcggtg atggcattgc
    26821 cgatatcgtc gggcggcgca tcgcctcacc caaactgccg tggagcaggg aaaaatccat
    26881 tgctggcatg gtcagcgttt ttgcgggcgg ctggttgatg gcaggcatta tgatctacat
    26941 ttatgtagcg gctggtgttt tttccgctcc atttgcccaa tacactttgc cgatcacgct
    27001 cattgcgctg gcgggtgcag tggtcgagtc actgcattac aaggacattg acaatgtgac
    27061 catgacactg gcctcggctt tgatcggcgg tttgtttttc taagaaagcg cctgaaagtg
    27121 ttttcgtgtc tacgtgattg aattttcaag ctttaggaat cagccgcgaa ttattttaaa
    27181 agtctgaaag attacccacg gagccacaga gaaaaaataa aaactccgtg actccgtggt
    27241 tcaaaatggt tgttcaaaga aattccctgc aacgtatgtg aattgcaaga tacggacaag
    27301 atataaaaag ccgaccctgt aaaatggatc ggctttttcg tgtaaatccg cggctaaaga
    27361 attgaatgat tagattcggg atgtttcata ttttgcgtaa gaacttaagc agagaatcgg
    27421 ctactcgccc ggtatatcat cctctttttt caggatcatc actttataag attcggcaaa
    27481 ctcgaggccc ttttcctttc cttcctcttt ggcccactcg tgcatccaat catccacatc
    27541 atgggtgtca gactggctga catgtttctt cagtgcttcg atcttgatat cgatggtttc
    27601 gctgatatca acccaggtat cgggcttttc cgacccgtgg atatacaatc gcttgacgtt
    27661 gtgcggttca taccccgcct caagcaggtc ggtgaaaata agccttgtcc cagcggaagg
    27721 gaagaccgcg tacgcggctg cttctgccgc agcacgatga tccgggtggt tgatgtatcc
    27781 gctgccgtag aaccaggccg aagggtcgcc tgaaacgact acgtcaggtt tgtatatgcg
    27841 gatgaggcga gtcaggtctt tgcgcagggc aatgctggcg gtcaattctc catctggata
    27901 atgaagaaaa accgtttcag aaattcccag caccgcattg gctgcgagtt gttcgccttc
    27961 gcgcagttct gcaagcacgg atttatagtc ggcacctttg gtcacatcgt ttgaaccaga
    28021 gtcgccgctg gtgataagca cggagatgat cttgcatccc gctttggccc atttggccag
    28081 cgtacccgca acagtaaagt cttgatcatc gggatgcgcc tgaatggaca ttgcaatctt
    28141 tggaagatat tcctgttcgt ttgacatggc tgtgatttta ccagaggctt aaagtcagag
    28201 cgccaggggc tgccgaccct ggcgctcttt ctacaaggag gagaagagaa atgagcttgt
    28261 gcctcctact ttatcacagg cattcaagca ctacttgacg ccaaccctac gcctctccca
    28321 tgattatgca acgtcttccc tgcaatgaac cgctgaccag caaaaccttt gtgactgcag
    28381 gcaggctggt gattttgaaa cgcaaataat gcgaacagcg agttttcata atttatccaa
    28441 gttgccattt gcgcaaaagc agggaaaaga aaaacgttag ccgcgaatgg cattaaaaaa
    28501 ttcacggctg aaaacttgat tttgatcaca gagaatcaac ttgctaattg ctttactcgc
    28561 agcattcaca ttaaagcttt ttgactttgc aacggtcaca gcagactcta tatggctttc
    28621 tcaaattaat atctgacaag cctttcgaac cgccaagccc gcgaagaacg ccaagaaaat
    28681 ccattaaact tcgcgctctt tgcggttagt tttgtctatg ttgggccagt actcccgact
    28741 ttgagaaagc catagcagac tctacatcca tgcttgacac atccacaaat tgctcgtaga
    28801 ctctcaatta agttctgcct taatcgaaag atacccatga aaactgtcca acccattcaa
    28861 tcctttgacc agatcaaatt gctggcagat gcacgccgaa tggaaattct gcgcctgctg
    28921 atggcggccc ccgccaccct cacaattctt gcgcaaacca tgaagcagtc gcctgcatgg
    28981 gtgcggcacc acatcctcgc gcttgagtct gcggaactga tcgaagtttc tgaattgcgc
    29041 aaaacaggaa aagtgaccga aaaattctac cgcgccaaag ccgcggcttt tcttttacag
    29101 gaagtggtcc tgccgaaaag caaaaagcaa tccattatct tctctggcag ccacgatctg
    29161 gcgctggaag agattgccga acacctcaat aaacatgtca ccttgttgag catgcccgtc
    29221 ggctcgctgg atgggctggt caatttacgt caggggctat gtcagatctc cggggcgcat
    29281 attttggatg caacaggcga atacaacacc cccaccgtcc gtcacttgtt cccagaccgc
    29341 aacgtggaat tggttacgct ggcataccgc acgcagggct tgatcgttgc cccaggcaac
    29401 cccaggaaca tcaaaaaaat atccgatctg gctcgtgaga atgttaaatt catcaatcga
    29461 aattcgggct cgggcacacg catctggttc gacaacgaac tgaagaaact aaaactcgac
    29521 ccgacacaaa tccccggtta cgaccaatcg gtcaaaaccc atagtgaagc cgcacaggca
    29581 gtcttcacca aaactgccga tgtggccctt ggcctgcaag ccgccgccaa acaacacgac
    29641 ttggatttca tccccctctt tgaagaacgc tacgacctga tcctgccgcg tgaaaatgaa
    29701 agcctgcttt caccgctttt ggactacctg caaacttcaa ccttccgctc atcgctgggc
    29761 aggctggtcg gttacaaccc aactcacagc ggcgaacaga tcgccgtgta aatcaaataa
    29821 ggagaaacgc catgaagctc agcgcaagaa atgtcatcaa aggcaaagtg acaaagatcg
    29881 tcaaaggagc agtcaattct gaagtgacta tcgaaattgc aggcggcgcc caaatcgttt
    29941 ccatcatcac aaactcttct gtaaaaagcc tcggtctcaa aaagggaaag gatgcctacg
    30001 ctgtgatcaa agcctccaat gtaatgattg ctgttgacta aaaatgaaaa aactcctgat
    30061 cgtcctttcc atttttcttt tgaccgcctg cggtgcgcca cccaccgccc cgataactgc
    30121 tgaagcgtca gtgacagaaa tctcagcctc agccgagccc accgcagaac caataaccga
    30181 gccctcctca ccagttctgt tcaagatcgt caaagcggat gggacaacat tcgatgtcac
    30241 tctcgaagct gtcaaaagcc tgccgctcac ccagatcaca gtggacggaa aagtgcagga
    30301 aggccccaaa ttgcttgaag tactggccct ggctggtgta acagaattta ccgaagtcag
    30361 cttgagcggt acaggttcac catccacgct cacattcaag caagtggacg ataacaccat
    30421 tctcgacttt agcaatcgtg gcaccatgaa acttgccacc actcacatcc ccaaagccga
    30481 ttggacaaaa gacattgctg aaatcacggt gaaatgatcc tcaagtgttc gtgctttttt
    30541 attttgcatt atccgttaag taaatactta gatgaatctt gaacaaaagg tgaaggaatg
    30601 cattatctga taggaattga cgataccgac aatctcgaat cgcgcggcac aggtcaccgc
    30661 gttcgtcaac tcgcagattg gctgactgaa aacaaactgg cttcaccact tggcatcact
    30721 cgtcatcaac tgttggtaga ccctcaaatt ccatacacct cacacaatag ttcagcctgt
    30781 ttatccgtag aaacgaacaa acccgatgac gtttgggaag cctcacagga attcctgttg
    30841 cgtgaaagtg cgcaaggctc cgatgcgggg ctggccctcg caaaatggga tgaggtcgac
    30901 aaatgcgtac tatcttttgc tggccgcgca aaactcacag tgctgaccat gcttgaagca
    30961 gagcaaacag cttcacagtc tggaataagc ctcgttggcc tcacaggcac acacggcgga
    31021 attatcggcg cattatccgc agtgggattg catcgtgctg gcaatgatgg tcgctttcta
    31081 tggctgcccg gtctgcgtga tctaaaaggt aaatatcctg tttccgaaat ttatgcaaaa
    31141 ggtcatgtag accgcgtatg cactttgagc catgtggatc tacataccaa aaccattgtt
    31201 gacgttggag aatgggttcg tcccattctg cgtgaaggca aggccacatt atatgttgag
    31261 gagatcaatc atgaatggca catcatccct aaagaatata tcaaaaagct ttccaactga
    31321 atgggtactc ccctggtggg aggccctgct tttgatcggc ggcggcgtaa cagccgtggt
    31381 tttgcatcgg gcttatgacc tatcccttgg tatgcctggt catcacggca tcgagtggat
    31441 ggcactgatg atcatcgggc gtgcatcttc acggtttcgc ggtgcgggtt cgctcacaag
    31501 cattggcgct tcatttgcat ccacactgcc gtttttacaa ggcgaaaatc catttacctg
    31561 gctgttctat ttgttacccg gcccagtcat ggacctcgcc taccgctatc ttccgcgtta
    31621 cgcaaacaaa ctttggttca tgatggtact tggcgggctg gcacatgcca ccaaacccat
    31681 tggtcaactg acaatgaacc tgatcactga ctggccgttt ggctccttcc gttatggagt
    31741 ggtattccca tttgtcagcc attttttctt cgggatgatc ggtggattac tcggcgcgtt
    31801 gatcgtgctg ggcatccagc gtatttcacc aaaatctgaa aagtgaggaa tggaaagatg
    31861 aaacaaacac gcaacatttt attaaccctc ggcttgatcc tggcgatt
//
LOCUS       DAOQLC010000041        31623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_448_length_31623_cov_26.882793, whole genome shotgun sequence.
ACCESSION   DAOQLC010000041 DAOQLC010000000
VERSION     DAOQLC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 31623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_448_length_31623_cov_26.882793"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PL000_09175"
     CDS             complement(<1..633)
                     /locus_tag="PL000_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNC89113.1"
                     /translation="MNKSSSPLLFLGKADDPDCARALEFCQSHFEHVTFCLGKWGDPL
                     PETARNWEGDIIISYLSRWVVPADLLNRAKIAAINFHPASPEYPGIGCNNFALYENAK
                     EYGVTCHHMASKVDTGRIVAVKRFPIFPEDDVDALLKRTYENQIALFFEIANLLAEGK
                     ELPTSAETWTRPPFSRKQFNELFIITPDMNEDEVNRRIRAISYGPWQPYME"
     gene            complement(740..1306)
                     /locus_tag="PL000_09180"
     CDS             complement(740..1306)
                     /locus_tag="PL000_09180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC89114.1"
                     /translation="MFPFLKLTDGTITLRPFEFGDEKDLRDAVQESLPELQPWMSWAT
                     EKYNLETARNFIAITRGQWSSGSLYAFAIADAVTDELVGGCSLSHIHPVYYFCNLGYW
                     VRSSRHGEGIAGAAAKLAAHFAFERVGLIRTEIVIAVGNEPSRRVAEKIGAHYEGILL
                     NRMVVGQAIHDAHMYSLLPSDFGLTAKR"
     gene            complement(1448..2107)
                     /locus_tag="PL000_09185"
     CDS             complement(1448..2107)
                     /locus_tag="PL000_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89115.1"
                     /translation="MKKQRIILVDDHEVVRLGLKSLLERHPHFDVVGEAGSAREALEQ
                     VEALKPDVVVMDIRLPGTSGIDACEQIVSQFPNTKVLMLTSYAEDEMLFSAIRAGASG
                     YVLKQIGSEDLIKAIESVGRGEALLDPAVTQRVFQEVRRAVKEEEASAFAHLSQQEKH
                     VLLLVSEGKTNREIAKNLFLGEGTVRNYVSSILSKLSVNNRAEAAAYAVQHSLREYIT
                     L"
     gene            complement(2158..2619)
                     /locus_tag="PL000_09190"
     CDS             complement(2158..2619)
                     /locus_tag="PL000_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89116.1"
                     /translation="MKELYEYREKMIARLAQAAREFCAACEAFENPLTQVEGEWNLHQ
                     IASHVRDVEKLVYGDRIRKTLEMENPAFQSFDADSWMETNYNQNESLQAILSEFSSNV
                     DALCQTLKNLPQSAWSRESSHETMGGELALQLWVERGLAHIEEHLQTLKKA"
     gene            complement(2616..3215)
                     /locus_tag="PL000_09195"
     CDS             complement(2616..3215)
                     /locus_tag="PL000_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC89117.1"
                     /translation="MDLRNKVVLITGAGKGAGAALARAFAEQGALVALNDISPINVEQ
                     VAAEITAKGGKAKAYVEDIAKKVGVQYVIKGVEDDFGGMDILVNHAVVKPHTTLLDVD
                     EWDWHRTLDVNLTGAFLTIQSAGRIMREKGHGAILNIVAEAGIRPESEAGAYPASMAG
                     LVALSHQADRELSPLGIRVYAVENSADVVKNVIKILEAQ"
     gene            complement(3229..3924)
                     /locus_tag="PL000_09200"
     CDS             complement(3229..3924)
                     /locus_tag="PL000_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89118.1"
                     /translation="MSKRTIIPIFLLICIAGGLYYYFIGRRGSARTAEVYNWLRDSTS
                     RPELMMTTGTTCGDAPFIFPTNGLIGFIWDDSFRPGHRHSGIDIFAGTDVGITPIVTA
                     YPGYLTRKSDWKSSVIIRVPEDPLQPGRQIWVYYTHMADPNGNSYISPEFPTGTTEVF
                     VEAGTFLGYQGNYSGDPNNPVGVHLHVSVVKDDGFGNFTNELDINNTYDPSPYFGLPL
                     NANENTDTIPVCN"
     gene            4199..6067
                     /locus_tag="PL000_09205"
     CDS             4199..6067
                     /locus_tag="PL000_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89119.1"
                     /translation="MKRKLFSIIAVLVMMLGLIPATALAAPAPQEGFGGGGVTMFTQR
                     VPINVVFLGYDKNAINQKAIRDLLPASYTPIVRYPAFYGLEGRNLGLRFDFNYTVSFV
                     NQKTTDRFFKFLTQGSTVAGPTFYQNAYNDMENNVLDVADEVLYADAPSVESWLNQNL
                     NVNKKGYTIVFINWYGRPDFKFHVYTKTDVADPDTGYNFGEIRSSRKTIAWGGSSSRL
                     WFYDLSAGPEAWTGNYDVDNPDLDGNGVEDYRMPPIWEYSANGYRDPSALSTDLGLVA
                     RFVGINLLFTTSPLYDPLASAPEVNGSKIVHINMMEDDPASQGTDWINPGLAQQRFSE
                     FQPYYNWQVNLTDVNPIDANAEKSFRIWAELDGSDDCWNDWGTPYAQLFCYFDINRNA
                     YIPEYTDADYVAAFHEFNTTADNLGNQYGLLGFADDNWIDGTPSYVFAFDTDDYRAGG
                     YGFTITTIHEGGHHFGLSHPHDGYDSELGLDYGASDDFYFANSGDESNTIMHYMDLSD
                     EFGQFDRDNMYRIEMAGYLMWSNSLLNQILANPNAASVRGYISQAKNDGNRAVARFNQ
                     WNYLEAVSYARHAYEQLSLAAIQLGIPTPSSLYEPAMANPGMPVPHEGDPIRFPDN"
     gene            complement(6140..6595)
                     /locus_tag="PL000_09210"
     CDS             complement(6140..6595)
                     /locus_tag="PL000_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89120.1"
                     /translation="MKKIINILIFSGLLFLAACGGAAAPAAPNSENPPLATPPPVDSA
                     NEPEQPAAESPIEQNMVNLSLENLSRKLNIGVEQIRVTKVTKVVWRDASLGCPKPGVD
                     YLRVETPGFSITLEADGKSYELHTDESKRVTICNNAQPKGTTPRWKSLI"
     gene            6900..9152
                     /locus_tag="PL000_09215"
     CDS             6900..9152
                     /locus_tag="PL000_09215"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNC89121.1"
                     /translation="MKAKWFSTILILALLVMSLVPAAGAAPSLEDEGPIFAGKGDDNL
                     SHPMGDAQAALKAKAMEAMLQGKAKGKTHEVFRGQFAELALEKNDRVFVIVAEFGDAI
                     HPNYGGEPGPLHNQIAEPDRTVDNTTIWQADFDKAHYEDMYFNQMVDYYAKQSSGRYT
                     INGDVIDWVKVPYNEARYGSNFCGSTTCSTVWYLIRDAINQWVVDQKAAGMSNQEIHD
                     YLATFDVWDRYDADGDGNFDEGDGYIDHFQIVHAGVGEETGGGAQGEQAIWSHRWYTS
                     YQGIGVWGANGLGGMEFGGNGTFANEGGPNAVGIWVGDYTMQPENGGLGVFAHEYAHD
                     LGLPDQYDTAGGDNSTGFWTIMSSGSYLGDGSTDIGSRPGDFNAWEKFQLGWLDYTVA
                     KEGAKSEIVLGPAETTTQHAQALFVTLPDKEVTVNLATPYSGSYFYYSGAGNDLDNSM
                     TRSVTLPSGAASLTAQVNFQIELDWDYAYVTVNGSPVATNLSTNDNPNGQNFGEGITG
                     DTGGNWVALTADLSDYAGQTVDIGFNYWSDGAVAELGFMVDDVAITGQPLDDAESNFG
                     WNFNGFSATTGTETSYYFNAYVAEYRQYRTYDAGLQTGPYNFGFGNTMPDWVEHYPYQ
                     DGLLISYWDSSFTDNNTSEHPGGGLILPIDSHPNALIRPDGNPWRSRIQAYDSTFSIT
                     RTDGFTLHLNGEPSTIPSQPGVRLFNDALQYWNWTTPLAGVINPHTGTMIRIKGINAQ
                     GFMQVQVKAP"
     gene            complement(9215..9670)
                     /locus_tag="PL000_09220"
     CDS             complement(9215..9670)
                     /locus_tag="PL000_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89122.1"
                     /translation="MKTYGTLLFLSSLLFLAGCQPESSPVTNTALPAMSFSQTPPPAQ
                     EEGIEMFNEGTPVDPQAQKMIQLAKESLVKKFKLNEDQVHVASIQAMDWPDAGIGCPQ
                     AGMVYAEVITPGYQILFEANGQSYSYHTDTVNKVLLCRIHPVDDIFLTP"
     gene            9817..10200
                     /locus_tag="PL000_09225"
     CDS             9817..10200
                     /locus_tag="PL000_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNC89123.1"
                     /translation="MTIYIQPHQFRRMARRWAEQPQQRALGVNIREQDDAYVLSALVP
                     GLKAEDLNIQILENVVSIEGAYQESDGEYLLNELPSGSFRRALRLPTEVEADKAEAKI
                     ADGVLTLNLPKAETARPKKINISLN"
     gene            10336..11352
                     /locus_tag="PL000_09230"
     CDS             10336..11352
                     /locus_tag="PL000_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89124.1"
                     /translation="MRVKNFLFTILVIAILSACGGSATEVAGPTAVPTHTAVPTSTMI
                     PTPSTPLAILVLPADMDKASSDLYQKTVYDLAQQSGFRFQVRNTLTPVDLADPTLKVV
                     IALPPDPGIASFAPTAPNVQFLAVNIPNLIAGGNLSVLAGDSQVELPAFMAGYVGAML
                     SDEYHIGMVYPQGDADAQRAAVAFKNGMTYYCGLCKTFYISEIVYPTFVEVPVNEDPA
                     RYGGYANVLINDRKVYALYIYPSLANADLLSYIGTQGVMLIGTSMPETRPGGWVMTIR
                     PDTIKAIQTAWPNLIAGQGGQNVQSPLGIADVDPSLLTPGKLRLAQQTLESLLAGRIL
                     TSNP"
     gene            11500..12051
                     /gene="infC"
                     /locus_tag="PL000_09235"
     CDS             11500..12051
                     /gene="infC"
                     /locus_tag="PL000_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNC89125.1"
                     /translation="MNEGIRVAEVRLIGPDGGNVGVVPIRQALQIARDAEMDLVEVSP
                     GATPPVCRVMDFGKFIYEKAKKEREARKSQTKIEVKEIRLRPKTNGAHRGFKVDDARR
                     WLGQGHKVRVTVKFRGREMDYPEIALEDLREIVQDLVDVAVVEVPPQMEGRTMLVVLA
                     PAKGAGKKKEKSEQAEVKTEAEA"
     gene            12158..12391
                     /locus_tag="PL000_09240"
     CDS             12158..12391
                     /locus_tag="PL000_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNC89126.1"
                     /translation="MPRKAKKSGKIKLKTHKATSKRFRLTGSGTLVRTKGGKSHLRRR
                     TSDRTKALLSEMVVVKGRKFIKRIKRLAPHMEG"
     gene            12496..12837
                     /gene="rplT"
                     /locus_tag="PL000_09245"
     CDS             12496..12837
                     /gene="rplT"
                     /locus_tag="PL000_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002507379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNC89127.1"
                     /translation="MRVKGGPQGHLRHKKVLKITKGQRGSKSTLFKRANEAMLKSLWY
                     ATRDRRVRKRDLRKLWIARINAAARLNGTTYSKLVSALKKANIDLNRKMLADIAVRNP
                     QAFAAVVAKTK"
     gene            12898..13461
                     /locus_tag="PL000_09250"
     CDS             12898..13461
                     /locus_tag="PL000_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNC89128.1"
                     /translation="MNTILVERILPTGQVLQIVQGDITIETVDAIVNAANEHLAHGGG
                     VAWAIVRRGGEVIQQESDEWIKQHGPVSHAHPAWTLGGALPAKYVIHAVGPVWGDGGE
                     DGKLADAIHGSLQVADELKCKSMSVPAISTGIFGFPKDRAARIIFYAIEKYFVAVKTS
                     TVQTIRLVLFDHATIQVFLDAWNTANP"
     gene            13492..14277
                     /locus_tag="PL000_09255"
     CDS             13492..14277
                     /locus_tag="PL000_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC89129.1"
                     /translation="MITSNHNPRIKFVRALQGRARERRDAGAFVADGVRLVEEAANSN
                     WIFQFALYDETLNERGKILVENLKSRGVEVEEVSASVMKSLSETETPQGILAVLEFTP
                     LPVVPHFDFILIPDQIRDPGNLGTLLRTAAATGVQAVLIPPETTDAFSPKVLRSGMGA
                     HFRLPIIPKSWEEIEQVVEASSLQVLIADMGGNSCWETDLCQSLALIVGSEAEGASDA
                     ARKLADQQISIPMSGAVESLNAGVAGSILMFEVVRQRVVNNKQ"
     gene            14289..15512
                     /locus_tag="PL000_09260"
     CDS             14289..15512
                     /locus_tag="PL000_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HNC89130.1"
                     /translation="MKIRSITYFCNPKNPFDEKILRSAGDFLSRAKSAFEAGGYEVQT
                     VRLATTPFPNLLNGRVGDLPVIAQGLANLLPQIGIAYASLGPALIEFPESYAVIPDAI
                     AASENIFFGGVMADRKNGISMQAVKACAEVIQRAATIEANGFANLRFAALANVHAGAP
                     FFPAAYHDADKPAFAIAVESADVAVDAFAGQSDFSEGRSALVAEIQRHGQAIARIAVS
                     ELSSIRFLGIDFSLAPFPDDAHSLGNAVEKMGVPKIGLHGSLAAAAILTEAIERADFP
                     HTGFSGFMQPVLEDSVLARRAAEGTLTIKDALLYSAVCGTGLDTVPLPGDVTADEMSP
                     LLLDLCALALRLDKPLTARLMPVPGKRAGDVTEFDFGFFANSKVMRLDSAPLTPPFSA
                     SQNIHLNARKHESAG"
     gene            15496..16002
                     /locus_tag="PL000_09265"
     CDS             15496..16002
                     /locus_tag="PL000_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNC89131.1"
                     /translation="MNLLDEISFVRLLWGVPILLAIHNLEEAPFMEKWSKELGLPIHF
                     AISTRQFIWAVTLLTAAGFAATYLGINTTKQPIGVWAVLEIQMIMFVNAVVPHFAAFL
                     RFQRYNPGVVTALFLNVPFSVYLFQRAMQAGVMSWGVFWVLLVIAPFAAVVLIFTSLQ
                     FGKWVSRN"
     gene            complement(15999..16553)
                     /locus_tag="PL000_09270"
     CDS             complement(15999..16553)
                     /locus_tag="PL000_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC89132.1"
                     /translation="MSEELVAFFKALADANRLKIVGLLAQQPYSVEELAALLNIKPPT
                     VSHHLAKLAQVGLVSAKADSYYNVYQLDEKALETKSKSLFSQENLAASVANVDADAYD
                     NKVVKDYVRKDGSLKTIPAQKKKLDAILRYVVKAFELEKRYSEKKVNEILAQYHEDTA
                     SLRRELVGAGLMKREGGGGEYWRV"
     gene            complement(16550..17095)
                     /locus_tag="PL000_09275"
     CDS             complement(16550..17095)
                     /locus_tag="PL000_09275"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HNC89133.1"
                     /translation="METNPEMLDFVKAMSNPNRLRIIGLLSQNPLSRAEITARLNLSP
                     KEALNHLTFLEHVGAVLQTEEIYTLNDDRLATLAREKLAEERPVFIPAPDLDEKSKKV
                     LKAHLNADGSIKQIPAQPKLQAILNYLVGFFEFGTNYTEKEVNTIIRRFHIDTAGLRR
                     DLIEAGLLNRESDGSRYWRVA"
     gene            complement(17213..17587)
                     /locus_tag="PL000_09280"
     CDS             complement(17213..17587)
                     /locus_tag="PL000_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC89134.1"
                     /translation="MPTYDFICNKCEKRFEVFQTFSEYGKKTVHCTHCNSENVRRRMT
                     KVRIAKSESSRIETATDGLAGMEEIGDDPKALARMMKKMGNEMGEDIPTEFNEVVDRL
                     EAGQTPEEIESAMPVLGDTKNE"
     gene            17658..18275
                     /locus_tag="PL000_09285"
     CDS             17658..18275
                     /locus_tag="PL000_09285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNC89135.1"
                     /translation="MPTSFELRKPNPLLIVISGPSGVGKDSVVQRMKERGFPFHFVVT
                     ATTRDKRENETNGRDYWFVSKDEFARMIDENELIEYAIVYGDYKGIPKWEVREALASG
                     QDVVMRIDVQGAETVRKLAPEALLIFITCEGEDELERRLRERKTETADSLSLRIATAR
                     KELQRIESFDYVIVNEDFHLDDTVDKVRAIIAAEHLRVQQRKVTL"
     gene            18272..19051
                     /locus_tag="PL000_09290"
     CDS             18272..19051
                     /locus_tag="PL000_09290"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC89136.1"
                     /translation="MIEPPASPPTDVPVPQPQAVSVRLPFTTPTVTYVLLGFTVLVYV
                     LQLLSVAVFGYAVYEIDWLEVYGARFNEAIRMGELWRFITPVFLHGSMAHIFFNMYAL
                     LSIGSFLERHFGHGRFALLYFLGAFAGNVFSFLLTGENGYSIGASTAVFGLIAAEVIF
                     FYQNRKLFGGQARQAISNAGFIIAVNLFIGLAPGIDNWGHVGGLFGGAIFTWFAGPFW
                     MVVGTPPDFHLQDRREMREIIVGAAAVCVIFGALGIFGMFS"
     gene            complement(19182..20138)
                     /locus_tag="PL000_09295"
     CDS             complement(19182..20138)
                     /locus_tag="PL000_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89137.1"
                     /translation="MTTQSVSPKKLDAEALSLDSVSLWQITRRRLFRRKSSIVGMVIL
                     GLLIFIALTAQWLAPYDPTLSMLDADPPQKVEKRASPCIHALGCPADQPQHIMGTDGN
                     LRDEFSRMLYGARLSLMIGFSTVTFAIIIGTVLGALGGYFGGWIDNIIMRVMDVLLAF
                     PSLLLAIAIVTVIGPGLINALLAIGIVSIPAYARVVRGSVLSVREMDYVSATRALGGN
                     TFEILFKRILPNALTPLIVQGTLGIASAILDAAALSFLGLGAQPPTPEWGSMLGAERN
                     QVFTAPHLVFYPGLAIMLTVLAFNLLGDGLRDALDPRLAHTS"
     gene            complement(20148..21227)
                     /locus_tag="PL000_09300"
     CDS             complement(20148..21227)
                     /locus_tag="PL000_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89138.1"
                     /translation="MVTYILRRLVLSIPVLFGILFATFALARLIPGDPCRAMLGEKAT
                     KVVCDEFNHRHGLDRPILVQFGIYVNEIAHGDFGNSFRYSKPITELLAERLPITFELS
                     IAALAFSVIIGIPLGVIAAVRHNSWADVATMLWANVGVSMPVFWLGLMLAYIFSLTLK
                     DTIFQLPPSGRLSAGLITTPFFEVWGWQLQEGTFSFSLADFISRMNVFNSILSGNWEV
                     LKDASIHLILPAFALSTIPMALIARMTRSSMLEVLGQDYIRTARAKGLVRRSVILKHA
                     FRNALLPLATVIGLSLGGLLGGAVLTETVFNLSGVGRILYDGITARDYGIVQGFTVVI
                     AIFFVILNLIVDVSYAYLDPRIRLD"
     gene            complement(21290..23041)
                     /locus_tag="PL000_09305"
     CDS             complement(21290..23041)
                     /locus_tag="PL000_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89139.1"
                     /translation="MKRNLSVLLSLVVLASIMLAACGGGAATEAPATEAPAATEAPAA
                     TEAPAATEAPAAYEGMKVESPDCDYGGEFKSIEAVDEFTVKITLCVPDPAFPSKIAFT
                     SFAIQPAEYLEASGGNGDLLEKPIGTGPYMVESWERGNQLVLKANPNYWGEFSGAETL
                     VFKWGTESAQRLLELQSGTVDGIDNVGPDDFATVEGDANLALFNRPALNIMYIGFNNN
                     PQVEGFDNSTNPLANEKVRQAIAMGVDRQRIVDNFYPAGSEVASHFTPCSIPNGCVGD
                     EWYSFDPEAAKALLAEAGYPDGFETVLNYRDVVRGYLPDPNVVAQDLQAQLKENLNIT
                     LNIEVMESGAFLAAADAGQLQGLYMLGWGADYPDQTNFLGYHFGAGASKQFGDKFDDI
                     VEALDKGAQLASDAERAPFYEAANNAIRTHVPMIPVAHGGSALAFKASVEGAFASPLG
                     NEEFSVMNNGTDTFVWMQNAEPISLYCADETDGESLRACEQVTQSLLAYETGGTAVEP
                     ALAESYDVNADLTEWTFHLRQGVLFHDGSSLDANDVVTSLSIQWDAANPLHTGNTGAF
                     SYWSGLFGTFLNAPPAQ"
     gene            complement(23137..24249)
                     /gene="amrS"
                     /locus_tag="PL000_09310"
     CDS             complement(23137..24249)
                     /gene="amrS"
                     /locus_tag="PL000_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNC89140.1"
                     /translation="MSSLAKRLDGLTVEGKLYEALEGGDVRCLACGHRCLIREGRRGI
                     CQVRFNRDGKLFVPHGYVAALQSDPIEKKPFSHVLPGVNALTFGMLGCDYHCGYCQNW
                     LTSQALRDPASDVSAQFIREVSPQTMVDHAKRTNASVVVSSYNEPLITSEWAVEIFKA
                     ARANGLLCAYVSNGNNTPEVMKYIAPYLDAYKIDLKCMSDKGYRKLGGTLQHTLDGIQ
                     RAREMGLWVEVVTLVVPGFNDSNEELWEAARFLAGLSVDIPWHVTAFHKDYKMTEPDN
                     TDGNTLLRAAEIGREAGLRYVYAGNLPGNVGEYEDTHCPQCSYQLVRRSGYVIQSYQI
                     TGDGKCPKCGEKIAGIWPSDPSTVGLYGVGRPFSVW"
     gene            24315..24917
                     /locus_tag="PL000_09315"
     CDS             24315..24917
                     /locus_tag="PL000_09315"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNC89141.1"
                     /translation="MKQAITVVIQAGGMSSRMGEDKALKPFLGRPLIQRVIDRLAPIA
                     DEVIVTTNRPDEYAFLGLRLIADLKPGRGALGGLYTAVASAAFSQVAVVACDMPFASD
                     VLFEGARRLMVEEDADVVIAKTDEGYEPFHALYRRETCLPAIEAAIEADQWKVISWFS
                     KVKVRELLPQEVKAFDPSGLCFWNLNTPEEFAEAEKRASG"
     gene            complement(24907..25329)
                     /locus_tag="PL000_09320"
     CDS             complement(24907..25329)
                     /locus_tag="PL000_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC89142.1"
                     /translation="MFKKILLATDGSEHALHATQIAAELARNPTAKELRIVVAYDVIP
                     PYFGEPNMQFAINARMEEAQTILRKTIQTIGSIPCEIHTELIEGSAAEAIIDVAATRQ
                     SDVIVMGSRGLGKLAGLLLGSTSQKVVAYAPCPVLVTR"
     gene            complement(25464..27416)
                     /locus_tag="PL000_09325"
     CDS             complement(25464..27416)
                     /locus_tag="PL000_09325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC89143.1"
                     /translation="MITNPVTSETSLKSRPGLRRALLGVWSVVVLYIVYVLGEAIAPQ
                     FGNAMKASMAQNLLGISSLLQIGFYYIVDILLVLGFSVIGVMIVMHSSDDWFAIFTSI
                     FLITFGVRVTDLANAIALQPGTEFAGGLLLAMGDIGIVLFTMLFPDGKFTPRWLRFLA
                     IPLVVTMLGIYLFPSAPFFWDKLGQGYLLITTSWYLIGFFTLLNRYFRKATLAHKQQI
                     RWVFIGTLGPFLWFIIFQVGLSFLPKPAESSLTMTAILLVSRLLSIFLFLVLPLSISI
                     SIAQTKLFNIDLIINRSLVYGSMTLALGLTFAVMLGLISLVFKNFHQGDQSLVAATIF
                     SVSAGALFQPARKKLQRFVDRFFYHIEIDYQKTPSDITLSPDSSSTTPRFSSYNDLRL
                     IGRGGMAEVYRATSQTKNKPVAIKVLRSALASDDSFRKRFMREAEIIARLQHPNIVNI
                     MDFGDENGVYHIVMEHLSGPDLNNLIKQEGRITLPKTISILRGVADALDYAHQNGLVH
                     RDIKPSNVMLDSSTIPPRVVLTDFGIAKIVDSQTLMTNTNMLGTLDYIAPEQIQASAE
                     VDSRADIYALGIMTYQMLTGTLPFEHASTGALLLAHLNAPPPDARTIVRDLPRNVAHG
                     IQRAMAKKPESRPSTAGEFITILENA"
     gene            27604..28872
                     /locus_tag="PL000_09330"
     CDS             27604..28872
                     /locus_tag="PL000_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HNC89144.1"
                     /translation="MTIDVSAIRKQFPALDRPATFFDNPGGTQIAKHSLDRINRYLIE
                     SNANHEGAFATSIASDAVLDEAHRAMADFYNASSPEEIVFGNNMTTLTLHLSRSISRD
                     WKEGEEIVVTRLDHDANVSPWVLAAQDRGVKVNWVDFDVEDGTLKLDQLQAALERKPR
                     LLAVGYASNSLGTINPVQKIIQMAHDAGTQVYVDAVQYAPHGPIDVQRLDCDFLVSSA
                     YKFFGPHAGILFGKHELLEKLFAYKVRPATNKLPGKLETGTQNHEGIAGVLGAIEYFE
                     WVGREFGGEHAESFAGSGYTGRTLTLKKAMTAIRAYEHEMNRALLSTLEAVPGITIFG
                     LTDTRRLDERVATFSFRLKNLHPRKTAELLAEKGIYVWDGNYYAINVSERLGFEGNGG
                     MVRVGAAHYNTLDEIARLGDVLKEIAEKAA"
     gene            28900..29910
                     /locus_tag="PL000_09335"
     CDS             28900..29910
                     /locus_tag="PL000_09335"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNC89145.1"
                     /translation="MVFQIVLTTVLALVFSFVIAPLSISLAWRFHLIDDPGLAARKIH
                     KAPMPRAGGTIIFLAILAGGALSGVLTTRPFTSIILASMIVFAFGIWDDIHNIRAIWK
                     LLGQILATLILIWQGVVVLFPGNTALNIAITLFWVIGITNAFNLVDSMDGLAVGLAGI
                     TVSFLMIGALYAGQNTLALFCAVLLGACISVFHLNFSLSRLFLGDSGTQLLGFTLAAI
                     AIVYTPPHLPQASSWFVPILLFGIPIFDTTLVTFSRMRKKMPVYKGNRDHTFHRLVDL
                     GATPHRAVLSIHMVAILLDCLAFFALSLSPLWANITFGVILLSGLFAILWLEANQKVE
                     TL"
     gene            29926..30825
                     /gene="speB"
                     /locus_tag="PL000_09340"
     CDS             29926..30825
                     /gene="speB"
                     /locus_tag="PL000_09340"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNC89146.1"
                     /translation="MTVEGTHGLWGDLHRPDLDPKQADFSVVGIPFDGLASARKGAAL
                     APERLRYWSRHLTPFSEDRTRLKDITLCDLGDISIVNPATDFNMIRQRVATLPNIPIM
                     LGGDHSVSIPIFQGQRERYQDKRLGVLWIDAHPDLCDTFDGSKLSHACVLRRGIEFGI
                     EPHDICMVGLRSWEDQEIDIVESGGVHVYTAADVAERGIRKVADSVRNILNDCDAIHV
                     SLDIDCLDPSAAPGTGIPEFGGMTSRDVLTLLKSMQGLPLVGLDVVEIAPPLDPSEAT
                     VFAGLKIIMEFIAVIAREKQKGL"
     gene            30827..31111
                     /locus_tag="PL000_09345"
     CDS             30827..31111
                     /locus_tag="PL000_09345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89147.1"
                     /translation="MPANSLNVTHNTDKNRFEVQIDSLTAELVYRLNGNVILFLHTGV
                     PPALEGRGIGSLLVKTGLQYAREKNLKVQSLCWFVSGYIERHPEFQDLVG"
     gene            complement(31165..>31623)
                     /locus_tag="PL000_09350"
     CDS             complement(31165..>31623)
                     /locus_tag="PL000_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNC89148.1"
                     /translation="GNPILGDDGVGWKVAEEVRAQLSEAGLDVDVECLSLGGISLMEH
                     LIGYSRVILVDSFALDEPIGSILILKLDDLPNYSAYHTTSPHDTSLQTAIALGKTMGA
                     WLPDEVMVVGIATKRIHEFSEELSPPVAEAVPLAGKFVMELIREKMNIEE"
BASE COUNT         7432 a         7721 c         8460 g         8010 t
ORIGIN      
        1 ttccatgtac ggctgccaag ggccgtaact gatagcgcgg atgcggcgat tcacttcgtc
       61 ttcgttcata tcgggggtga tgatgaagag ttcgttgaat tgcttgcgcg agaagggcgg
      121 acgggtccag gtttcggccg aggtggggag ttctttccct tcggcgagca ggtttgcgat
      181 ctcgaagaac agggcgatct gattttcata agtacgtttg agcagggcgt ccacgtcatc
      241 ttcggggaaa atggggaagc gttttacggc gacgatgcgg cctgtatcca ctttggaggc
      301 catgtgatga caggtcacgc catattcttt tgcgttttca tacagagcga agttattgca
      361 gccaatgccg gggtattcgg gcgaggcagg gtgaaagttg atggcggcga ttttagcgcg
      421 gtttaacaaa tcggcgggca ccacccagcg tgaaaggtag gagatgatga tatcgccttc
      481 ccagttgcgg gctgtttcgg gcaatgggtc gccccatttg ccgaggcaaa aggttacatg
      541 ctcgaaatgc gactggcaaa attccagtgc gcgggcgcaa tcggggtcat ccgctttgcc
      601 gagaaagaga agaggggagg atgatttgtt catgggttga ttatatatgc cctcggtcac
      661 aaattgcgct ttcccgcttc tgaaaaggcg caatttgtga ccgtgatggg tataaacact
      721 cttgaagcgt tgactctgct tatcgtttgg ctgtcagtcc aaagtcggag ggaagaagcg
      781 agtacatgtg ggcgtcgtga atggcctggc ccaccaccat tcggttcagt aaaatccctt
      841 cgtaatgcgc gccgattttt tccgccaccc gtctgctggg ctcgtttcca acggcaatga
      901 cgatctcagt gcggatcagg cccacccgtt cgaatgcgaa atgtgccgcc agtttggcgg
      961 cggccccggc aatgccctcg ccgtggcgcg aactgcgtac ccaatagccg aggttgcaaa
     1021 aatagtagac cgggtgaatg tgactcaaac tgcatccgcc gaccagttca tcggtgacgg
     1081 catcggcgat ggcaaaggca tacaaactgc cgcttgacca ttgtccacgg gtgatggcga
     1141 tgaaattgcg cgcggtttca aggttgtatt tttctgtggc ccatgacatc cacggctgaa
     1201 gctcgggcag tgattcctga acagcgtcgc gcagatcctt ttcatcgcca aattcgaaag
     1261 ggcgcagggt gatggtgccg tctgtaagtt ttagaaatgg gaacatggct gggttgtatc
     1321 atcctctgac cgtggttttc aaaaaacgct gatcgtttca ctcaggacga gggcaaaata
     1381 aaaaaggcac aacctgcacc gagttgattc tccgtgtgtt gtgcctttgt tgttgaacgt
     1441 gcggttctta cagagtgatg tattctctca gactgtgctg cacggcgtag gccgcagcct
     1501 cggcgcgatt gttcacgctg agcttggaga gaatgctcga aacataattg cgtaccgtgc
     1561 cttcgccgag gaacaggttc ttggcgatct cgcggttggt cttgccttcc gataccagca
     1621 agaggacatg tttttcctgc tgactcaaat gagcgaaggc tgaagcctct tcctctttca
     1681 cggcgcggcg cacttcctga aagacgcgtt gggttacagc gggatcaagc agggcctcgc
     1741 cacgcccgac agattcaatg gccttgataa gatcttcact gccgatctgc ttgagcacat
     1801 agcccgaagc cccggcgcgg atcgcagaga aaagcatttc atcttctgca tacgatgtaa
     1861 gcattaaaac ttttgtgttt ggaaactggc tgacgatctg ctcgcaggca tcaattcctg
     1921 atgtgcccgg caggcggata tccatcacca ctacatcggg tttgagggct tccacttgct
     1981 ccagggcttc acgggcagag cctgcctcac cgaccacatc gaaatgcggg tgccgctcca
     2041 acagcgattt caagccgaga cgtaccactt catgatcatc tacgagaatg attctttgtt
     2101 ttttcatgcc gttattttac ctcgaaatat gcagaaaacg agccattttc acgtatttta
     2161 tgcctttttt agcgtttgta aatgctcttc aatgtgtgcc aacccgcgtt ccacccacaa
     2221 ttgcaaggcc aattctccgc ccatggtttc gtggctgctc tcacgtgacc aggccgattg
     2281 cggcaggttt ttcagcgttt ggcatagcgc atccacattg gaagagaatt cactcaaaat
     2341 ggcctgcaaa gattcatttt ggttgtaatt cgtttccatc cacgaatcgg catcaaaact
     2401 ttggaaggcg gggttttcca tctccagcgt cttgcggatg cgatcgccat ataccaactt
     2461 ttccacatcg cggacgtgtg atgcgatctg gtgcaaattc cactcgcctt ccacttgtgt
     2521 caacggattt tcgaaggcct cacatgcggc gcaaaattcc cgcgcagcct gggcaagacg
     2581 ggcgatcatc ttttcacgat actcgtacag ttctttcatt gtgcctccag tatcttgatg
     2641 acattcttca ccacatcggc tgaattttca accgcataaa cccgaatccc tagcggactt
     2701 aattcacgat ctgcctgatg cgataacgca accaaccccg ccatgctggc aggatatgcc
     2761 cccgcttctg actctggcct tatgcctgcc tctgccacaa tattcagaat cgccccgtga
     2821 cctttttcgc gcatgatgcg tcctgccgat tgaatggtga gaaatgcgcc cgtgaggttt
     2881 acgtccagtg tgcggtgcca gtcccattca tccacatcca acagggtggt atggggtttc
     2941 accaccgcat ggttgacaag aatatccatg ccgccgaaat catcttccac gcctttgatc
     3001 acatattgca cgccaacctt cttggcgata tcttcaacat aggctttggc tttgccgcct
     3061 ttggccgtga tctcagctgc aacctgctct acattgatgg gggagatgtc gttaagggct
     3121 accagcgcgc cttgttctgc aaaagcacgg gccagcgcgg ccccggctcc cttccctgct
     3181 ccggtgatga gaacgacttt gttccttaag tccataaagc ctccataatt aattgcatac
     3241 agggatcgta tctgtgtttt cgtttgcgtt caggggtagg ccgaaatagg gcgaagggtc
     3301 gtaggtgttg ttgatatcaa gttcgttggt gaaattgccg aaaccgtcat ctttgaccac
     3361 cgaaacatgt aaatgcacgc cgacgggatt gttcgggtcg ccggagtagt ttccttgata
     3421 gccaaggaag gtgccagcct ctacgaacac ttcggtggtg ccggtgggga attcgggtga
     3481 aatgtacgag tttccgttgg ggtctgccat atgagtatag tacacccata tctgccgccc
     3541 gggttgaagg gggtcttcgg gcacgcggat gatgaccgac gatttccagt ctgatttgcg
     3601 ggtgaggtaa ccggggtatg ccgtcacgat gggggtgatg ccgacatccg tccctgcgaa
     3661 aatgtctatt ccgctgtggc ggtgaccggg tcggaaagaa tcgtcccaaa tgaagccgat
     3721 caggccgttg gtggggaaaa tgaatggggc gtcgccacag gttgtccctg tggtcatcat
     3781 caactcaggt cttgatgttg aatctctcag ccagttgtag acttcggctg tgcgggctga
     3841 gcctcgcctg ccaatgaagt agtaatacag ccctccggca atgcaaatga gcagaaaaat
     3901 ggggatgatg gtgcgttttg acatccaatc attatactca gattggaaat cggccaagag
     3961 cgggattttc tcaaaaaata cttgcacatt ttatcggttg ggaggataat tgcagttgag
     4021 ttgtaatgat aaattacccg aaagaatatt tcagacataa aacaggcggg catgttatgg
     4081 gttcttttga gtcaattatt ttttcaaaag gtggagagga ggtcgaaccg taaattctga
     4141 cagtttgtct agtgaaagca tctgagtttc aatttctgaa tcaaaaagga gaattagtat
     4201 gaaacgaaag cttttttcta ttatcgcggt tcttgtgatg atgctgggtc tgattccagc
     4261 aacagcgctg gccgctcctg cgccccagga gggttttggt ggcggtgggg ttacgatgtt
     4321 tacacagaga gtgcccatta atgtcgtgtt tttgggatat gacaagaacg caattaatca
     4381 gaaagcgatc agagatctgc tgcctgcctc gtataccccg attgtgcgct atcctgcctt
     4441 ttatggcctt gaaggccgca accttgggtt gagattcgat tttaattaca cggtctcttt
     4501 tgtaaatcaa aaaaccactg accgtttctt caagttcctg acccagggca gcactgtggc
     4561 gggccccacg ttttaccaga atgcctacaa tgacatggaa aataatgtcc ttgatgtggc
     4621 tgatgaagtc ctttatgctg atgcgccttc tgttgagagt tggttgaatc agaatttgaa
     4681 cgtcaacaag aaggggtaca cgattgtctt catcaactgg tatggccgcc cggatttcaa
     4741 attccatgtg tataccaaga ccgatgtggc ggatccggat actggctaca acttcggcga
     4801 aatccgctct tcccgcaaga ccattgcttg gggtggaagc agcagccgtc tgtggttcta
     4861 tgatctttcc gctggtccgg aagcctggac cggtaactat gatgttgata accctgattt
     4921 ggatggaaac ggcgttgaag attatcgtat gcctcccatt tgggaatatt ctgccaatgg
     4981 ataccgtgat ccttccgctt taagcaccga ccttggtttg gtcgcccgtt ttgtggggat
     5041 caatctgctc ttcaccacct cgccgttgta tgatccactt gcgagcgccc ctgaagttaa
     5101 cggcagcaag attgttcata tcaatatgat ggaagacgat cctgccagtc agggtactga
     5161 ctggatcaac cctggtctgg ctcagcagag attttctgag ttccaacctt actacaattg
     5221 gcaggtcaac ctgaccgatg tcaacccgat tgatgccaat gccgaaaagt ccttccgcat
     5281 ttgggctgag ttggatggct cggatgattg ttggaatgac tggggcactc cgtatgccca
     5341 gttgttctgc tattttgata ttaaccgcaa cgcctacatc cccgaataca ccgatgccga
     5401 ctatgttgcg gctttccatg agttcaacac cactgccgat aatctgggta accagtatgg
     5461 tttgcttggc tttgccgatg acaactggat tgatggtacc ccgagctatg tgtttgcgtt
     5521 tgatacggat gattatcgcg ctggcggtta tggttttacc atcaccacca tccatgaagg
     5581 cggccaccac ttcggtttgt ctcacccgca cgatggttac gactcagaat tgggtcttga
     5641 ttatggcgcg tctgatgatt tctactttgc gaactctggc gatgaaagca acaccatcat
     5701 gcactatatg gatctgagcg atgagttcgg tcagttcgat cgcgataaca tgtaccgcat
     5761 tgaaatggct ggttacctga tgtggtccaa cagcctgttg aaccagattc ttgccaaccc
     5821 gaatgcggct tctgtccgtg gttatatttc acaggccaag aatgacggca accgtgctgt
     5881 tgcccgcttc aatcagtgga actacctcga agctgtgagc tatgcccgcc acgcttatga
     5941 gcagctttcg ctggccgcca ttcaactcgg aattccgact ccgtcttcgc tgtacgaacc
     6001 tgcaatggcg aaccccggaa tgcccgttcc tcacgaaggc gacccgatcc gcttcccgga
     6061 taactagtct tttgtaaata gtaaaacaaa gtaaagagcg ccccatgtga tgtccacatg
     6121 gggcgctttg ttttttgggt cagatcaggc ttttccagcg aggggtggtg cctttgggtt
     6181 gggcgttatt acatatggta actcgtttgg attcgtcagt atgaagctcg taggattttc
     6241 catcagcttc gagggtaatg gagaatcccg gggtctcaac gcgaagataa tccacacctg
     6301 gtttggggca tcccaagctg gcatcgcgcc acaccacttt tgtcaccttg gtaacgcgta
     6361 tctgttccac gccaatgttg agtttgcggc ttagattttc gaggctgagg ttgaccatgt
     6421 tttgctcgat cggagattct gcggcgggtt gttcaggctc attggccgaa tcaacaggcg
     6481 gcggggtggc aagaggcgga ttttcagagt tgggggcagc tggtgctgcc gcgccaccgc
     6541 aggctgccag gaataacagt ccactgaata ttaggatatt tatgattttt ttcatacgac
     6601 ctccgtttca atgttataga cgtttttcgg aagtgaaagt tcactgcgga caagggaagg
     6661 ctggtcgccc gggtttaatc tttaaattaa tcttgcatta ataccttttg tttgtgataa
     6721 ttgcatttgt gtcgaaaata attcgatttc taaatccttt tagtgtggga ttttagtagg
     6781 gttggtgagg attatcctac ctgaagggaa aggaggttca caacaaaaat tgagcataag
     6841 gaaattggaa gtacctgttt gcaagtaaga tcatcaaatt ttcgtgagga gaatgattta
     6901 tgaaagcaaa atggttcagt acgattctga ttttagcact gcttgttatg agtctggtgc
     6961 cagctgctgg tgcggcaccg tctttggaag acgaaggccc gatctttgca ggtaaggggg
     7021 atgacaatct ctctcacccc atgggggatg cgcaggcggc attgaaagcc aaggcaatgg
     7081 aagccatgct tcagggaaaa gccaaaggaa agacccatga agtgttccgt ggccagtttg
     7141 ctgaacttgc tttggaaaag aatgaccgtg tttttgtaat tgttgccgag ttcggtgatg
     7201 ccattcatcc caattatggt ggtgagcctg gtcctttgca caatcagatt gcagaacctg
     7261 atcgcacggt tgataacacc acgatctggc aggcggattt tgataaagcc cattatgaag
     7321 atatgtattt caatcagatg gtcgactact atgccaaaca gtcttccggc cgttacacga
     7381 tcaatggcga tgtcattgat tgggtgaagg ttccttataa cgaggctcgt tatggttcga
     7441 atttttgcgg aagcaccacc tgttccacgg tttggtattt gatccgagat gccattaatc
     7501 aatgggttgt agatcaaaaa gctgcaggca tgtcaaatca ggaaatccat gactatcttg
     7561 caacatttga tgtgtgggat cgttatgatg cagatggcga tggtaatttt gatgaaggcg
     7621 atggttacat tgaccacttc cagattgtcc acgctggcgt aggcgaggaa actggcggcg
     7681 gtgcgcaggg cgaacaggcc atctggtcac accgttggta tacatcgtat cagggaattg
     7741 gtgtttgggg tgccaatggc ttgggcggta tggaatttgg cggtaatgga acctttgcca
     7801 atgaaggcgg cccgaacgca gttggtattt gggttggtga ttacaccatg cagccagaaa
     7861 acggcggctt gggcgttttt gctcatgaat atgcccacga tcttggcctg ccagatcaat
     7921 atgacacagc tggcggcgac aactctactg gcttctggac aatcatgtcc agcggttctt
     7981 atcttggtga tggttcaacg gatatcggtt cccgccctgg tgacttcaat gcttgggaaa
     8041 aattccagct cggttggttg gattatacgg tagccaagga aggcgcaaag agcgaaatcg
     8101 tccttggacc tgcagaaacc accacgcagc atgcacaggc actttttgtg acattacctg
     8161 acaaggaagt gactgtcaat cttgccaccc cgtattccgg ttcatacttc tattactcag
     8221 gcgcaggtaa tgacctggat aatagcatga cccgcagtgt cacgcttccc agtggggcag
     8281 cctctcttac cgctcaagtc aatttccaga tcgaacttga ttgggattat gcctacgtga
     8341 cagtaaacgg cagccctgtt gccactaatc tgtccaccaa tgacaacccg aacggccaaa
     8401 actttggcga gggcattact ggtgatacag gcggtaattg ggtagccctg acagctgacc
     8461 tttcagatta tgctggtcag accgttgata tcggtttcaa ttactggagt gatggtgcgg
     8521 tagctgaact tggtttcatg gtggatgatg ttgccattac cggccaacca ctcgatgatg
     8581 cggaaagcaa ttttggctgg aactttaatg ggtttagcgc gaccacaggt actgaaacca
     8641 gttattactt caacgcctac gttgctgaat accgccaata ccgcacatat gacgccgggc
     8701 ttcagactgg tccctataat ttcggttttg gaaataccat gccagactgg gtcgagcatt
     8761 acccctatca ggatggtctg ttgatcagtt attgggactc gtctttcact gataacaata
     8821 ccagtgaaca tccgggtggc ggtttgattc tgccaatcga ctcacatcca aatgcattga
     8881 ttcgcccaga tggaaatccg tggcgttctc gtattcaggc gtacgattct acatttagca
     8941 tcaccagaac agatggtttt accctccatc ttaatggaga accttctaca attccaagcc
     9001 agccgggcgt ccgcttgttc aatgatgccc ttcaatattg gaattggact acaccgttgg
     9061 ctggtgtcat aaatccgcat accggcacga tgattcgcat caagggtatt aatgcacagg
     9121 gtttcatgca ggtacaggtg aaagccccgt aaacctaatg atgtaaattt gtttgattgc
     9181 ttccgccaca gattgtctgt ggcggaagtt tttattaagg agtaaggaat atatcatcta
     9241 ccgggtgaat acggcataaa agaactttat tcactgtatc tgtgtggtag gagtaggatt
     9301 gtccatttgc ttcaaacagg atttgatagc ctggagtgat cacttcagca tacaccatac
     9361 ctgcctgcgg acatccaatg cctgcgtctg gccaatccat ggcctgtatt gacgctacat
     9421 gaacttgatc ttcgttgagc ttgaattttt ttaccagact ttccttggca agttgtatca
     9481 ttttttgcgc ctgaggatct acaggggtac cttcattaaa catttcaatg ccttcttcct
     9541 gtgcgggtgg aggggtttgc gaaaaactca ttgcgggcaa tgcagtgttt gttacagggg
     9601 atgactcggg ttggcagcct gccagaaaca acagactact taaaaagagt agggttccgt
     9661 atgttttcaa agttagctcc aggtattttt aggacgtttt ttgtaaataa aaagttcact
     9721 catttctaac acaatttcta tctttcatcg acattttttt tatgcaccat cactaaaata
     9781 ggttcaattc aattattcgt aaaggagaac aaaaacatga ccatttatat tcaaccccac
     9841 caatttcgcc gtatggcccg tcgctgggcc gaacagcctc aacagcgcgc ccttggcgtg
     9901 aacatccgcg aacaggatga tgcctatgta ctttcggcgc ttgtgcctgg tttgaaagct
     9961 gaagatctca atatccagat ccttgagaat gtggtcagca tcgaaggcgc gtatcaggaa
    10021 tctgatggcg agtacctgtt gaacgaattg cccagcggat ccttccgccg tgcgttacga
    10081 ctgcccacag aagtggaagc agacaaggct gaggcaaaga tcgcggacgg tgtgttgacc
    10141 ttgaacctgc ccaaggccga aacagcccgc ccgaagaaaa tcaatatctc tctcaattaa
    10201 agtagacgtt ccaaattgga gacgcacctt gtgtgcgtct cttttatttt taacaacctt
    10261 ggtgagtttc tgccgatctg ctttcgtaaa ataaaacagt ataattcgtg cattcttaat
    10321 ttctggagtt cccctgtgcg tgttaaaaat ttcctcttca cgatcctcgt tattgcgata
    10381 ttgagtgctt gcggaggctc tgcaacggag gttgctggtc ccaccgctgt gcccacccat
    10441 acggcagttc caacttcaac catgatcccg accccatcca cgccgctggc aattctcgtc
    10501 ctgcccgcag atatggataa agcctcttcg gatctgtatc aaaaaacggt ttacgatctg
    10561 gcacaacaat ccgggtttcg ttttcaagtg cgcaacactc ttaccccggt tgatcttgcg
    10621 gaccccactt tgaaggtggt catcgcattg cctcccgacc ctggcattgc ctcttttgct
    10681 cccacagctc caaatgttca gtttcttgcg gtgaatattc ccaaccttat agcggggggc
    10741 aatttaagtg tcctcgccgg agactcacag gtggaactgc cagcatttat ggctggttat
    10801 gtcggtgcaa tgctttcaga tgaataccac attggaatgg tatatccgca aggcgatgcc
    10861 gacgcccagc gtgctgccgt tgcattcaaa aatggcatga cctattattg cgggttgtgc
    10921 aaaacctttt atatttctga aattgtgtac ccgacctttg ttgaagttcc cgttaatgaa
    10981 gatcctgccc gttatggtgg gtatgcaaat gtactcatta atgaccgcaa agtgtatgcg
    11041 ctttacattt acccttcact ggcaaatgcc gacctcctct cttatattgg cacccaggga
    11101 gtaatgctga ttggaacctc catgccggaa acacgccccg gcggatgggt gatgaccatt
    11161 cgtccagaca ccatcaaggc gatccagact gcctggccaa acctgatcgc cggacaaggc
    11221 ggacaaaacg ttcaatcgcc gctgggaatt gcagatgtgg acccaagcct gctcaccccg
    11281 ggaaaactcc gcctcgcgca acaaaccctc gaaagcctgc tcgctggcag gattctcacc
    11341 agcaacccat aatgaaggtt ttttcaataa aagcggcaca ttttattgac gactcgtata
    11401 ccttcatggt attattcgcc cgctaaaaac cagccgcaag aaggctggat ttgtttgata
    11461 aaccacgagg agcagcatat cagtactaat gaatttcgag tgaatgaagg catccgcgtc
    11521 gcggaagttc gcctgatcgg gccagatggc ggtaatgttg gtgttgtgcc gattcgccag
    11581 gccttgcaga ttgcccgcga tgccgagatg gaccttgttg aggtctcacc cggtgccacg
    11641 ccgcctgttt gccgcgtaat ggattttgga aagttcattt acgaaaaggc gaaaaaggaa
    11701 cgcgaggcac gcaagtctca gaccaagatc gaggtaaagg aaatccgtct gcgcccaaag
    11761 accaatggtg ctcaccgcgg cttcaaagtg gatgatgccc gccgctggtt gggacagggt
    11821 cacaaggtga gagtcaccgt aaagtttcgt ggccgcgaaa tggattaccc ggagatcgca
    11881 ctcgaagatt tgcgcgagat cgttcaggac cttgttgatg tagctgttgt tgaggttccc
    11941 ccgcaaatgg aaggccgtac gatgctcgtg gttttggctc ctgccaaagg cgctggaaag
    12001 aaaaaagaaa agagcgaaca ggccgaagtg aagacagaag cggaagcctg agagttgtga
    12061 aatacctgta agagtgcaag tcccctaaga tgttgatcgt ccgcgggcgc aatcatatgt
    12121 cccgcggttt attttgtctg aaaggagtat cccagcaatg cctcgcaaag caaaaaagtc
    12181 tggaaaaatc aagctgaaga cccacaaagc gacatccaag cgatttcgtt tgaccggctc
    12241 cggcactctg gtgcgcacca aaggtggtaa gagtcactta cgccgccgta catcggaccg
    12301 caccaaggcg ttattaagcg agatggtggt cgtaaaaggc cgcaagttca tcaagcgcat
    12361 caaacgcctt gctccccata tggaaggcta gtccttccgg ttgtcgtcat tttttagttg
    12421 cggctttcgg gtgtgcgcaa ctggtgtacg cttctggcgc accgacgccc gcgagtttgc
    12481 aggaggtaaa gtaacatgcg cgtaaaagga ggcccgcaag ggcatctacg tcacaaaaaa
    12541 gttttaaaga tcacgaaggg ccagcgcggc tcgaagagca cactgttcaa gcgtgcgaac
    12601 gaggccatgc tcaaaagcct gtggtacgcc acccgtgatc gccgtgtgcg caaacgcgat
    12661 ctgcgcaagt tgtggatcgc ccgtatcaac gccgcggccc gcttgaacgg aaccacatat
    12721 agcaagttgg tttccgcact caagaaggcg aacattgatc tcaatcgcaa gatgctggcc
    12781 gacattgcag taagaaatcc gcaagccttc gcagcagtag tagcgaaaac aaagtaaaaa
    12841 gttgtagagc gagaaaattt tctcgctcta ctttttttaa cggggtaaaa tcaagtaatg
    12901 aataccattc tggttgagcg gattctcccc acaggccagg ttttgcagat cgttcagggt
    12961 gatattacga tcgaaactgt ggatgccatc gtcaatgcgg cgaatgaaca ccttgcacat
    13021 ggcgggggtg tggcctgggc gatcgtgcgg cgcggcggag aagtgatcca acaggaaagt
    13081 gacgagtgga tcaagcagca cggacccgta tcacatgcgc atccagcctg gaccttgggc
    13141 ggggccctgc ctgcgaaata cgtgatccac gctgtgggcc cagtttgggg agacggcggc
    13201 gaagacggaa aactggcaga tgccatccac ggttctttac aggttgccga tgaactgaaa
    13261 tgtaaatcca tgtccgtccc tgcgatttcc actggtattt ttggctttcc gaaggaccgt
    13321 gcagcccgaa tcatttttta tgctatcgaa aaatattttg tggctgtcaa aacctccact
    13381 gttcaaacca tccgtttggt tctgtttgat catgccacta ttcaggtgtt tcttgatgcc
    13441 tggaacacgg ccaatccata atacagccat ccattatttt ttcctgtcta catgatcaca
    13501 tccaaccaca atcccagaat taaatttgtc cgtgccttgc agggacgtgc ccgcgaacgc
    13561 cgcgatgcag gcgcatttgt tgcagatggg gtgcggctgg tggaagaggc ggcaaacagc
    13621 aattggatat ttcaattcgc cttgtacgat gagacgttga acgagcgtgg aaaaatactg
    13681 gttgaaaact tgaagtcacg cggcgttgaa gtggaagagg tctcagccag cgtaatgaaa
    13741 tccttgagcg aaactgaaac cccgcagggt attttggcgg ttcttgaatt taccccattg
    13801 ccagttgtac ctcactttga tttcatcctc atcccagatc agatccgtga cccggggaat
    13861 cttgggacgc ttttgagaac tgcagcagcg acaggtgtgc aggctgtgct gattccgcct
    13921 gaaacaacag atgcattttc gccgaaggtg ctccgatcgg ggatgggagc gcattttaga
    13981 ttgccgatca ttcccaaaag ctgggaggaa attgaacagg tggttgaggc ctcaagtttg
    14041 caggttttga tcgcagacat gggaggcaat tcatgctggg aaacagatct gtgccagtca
    14101 ttggcgttga tcgtggggag cgaagccgaa ggtgcaagtg acgcggcaag aaaattggcg
    14161 gatcagcaaa ttagtattcc aatgtcaggg gcggtggaat cgttaaatgc gggtgtggct
    14221 gggagcattt tgatgtttga agtggtaagg cagagggtag taaacaataa acaataaaca
    14281 gagagactat gaaaattcga tccattactt atttttgcaa tcctaaaaat ccatttgatg
    14341 aaaagatcct gcgcagcgca ggagattttt tgtccagggc gaaatctgct tttgaagcgg
    14401 gtggttacga agtgcagact gtgcgtttgg cgacgactcc atttccgaat ttgttgaatg
    14461 gcagggtggg ggatttgcct gtcatcgcgc aggggttggc gaatcttctg cctcaaattg
    14521 gaattgccta tgcttcgctt ggcccggcgc tgatcgaatt tcctgaaagc tacgccgtga
    14581 ttcctgatgc gatcgctgct tctgaaaaca tttttttcgg cggtgtaatg gcagatcgga
    14641 aaaatggcat ttcaatgcag gcggtcaagg cttgtgcaga agtgattcaa cgggccgcaa
    14701 cgatcgaggc aaatggattt gccaatttac ggtttgctgc tttggccaat gtgcatgcag
    14761 gggcgccgtt tttccctgct gcatatcatg atgcagataa accagcgttc gcaattgcgg
    14821 tagagtctgc tgatgtggcg gtggatgctt ttgcggggca atcagatttc agcgaagggc
    14881 gaagcgctct tgtcgcggag attcaaagac acggtcaagc catcgcccga atcgcggtat
    14941 ctgaactttc gagtattcgt tttcttggaa ttgatttttc ccttgcgccg tttcctgatg
    15001 atgcgcattc actggggaat gcggttgaga aaatgggcgt gccgaaaatc ggtttgcatg
    15061 gttcgttagc cgccgctgcg attcttacag aagccatcga gcgtgctgat tttccgcaca
    15121 caggtttcag cggattcatg cagcccgtgt tggaagattc tgtgctggct cgccgcgctg
    15181 cggaaggtac gttgacgatc aaggatgcgc tgctttactc ggcggtgtgc ggcacagggc
    15241 ttgatacggt accgcttcca ggggatgtga cggcggatga aatgtcgccg ttgttgctgg
    15301 atctgtgtgc gctggcgctg cgattggaca agccgctcac tgcgcggctg atgcctgttc
    15361 ccggtaaacg ggcaggagat gtcactgaat ttgatttcgg gttctttgcc aacagcaagg
    15421 tgatgagatt ggattccgcc ccattgaccc ctccattttc agcaagccag aatattcact
    15481 tgaacgcacg caagcatgaa tctgctggat gaaatttcat ttgtccgatt gctgtggggt
    15541 gtgcccattc ttctggcgat tcataatttg gaagaagcgc ctttcatgga gaagtggtct
    15601 aaggagttgg ggctgccgat ccattttgcg atatccaccc gtcaatttat ctgggctgta
    15661 actttgttga ccgcggcagg ttttgcagct acgtaccttg gaattaatac aacaaaacag
    15721 cccatcggag tatgggctgt tcttgaaatt cagatgatca tgtttgtgaa tgcggttgtg
    15781 ccgcactttg ctgcttttct tcgttttcag agatacaacc ccggtgttgt cacggcttta
    15841 tttttgaatg ttccattttc tgtttatctt tttcaacgtg ccatgcaggc gggggtaatg
    15901 tcatggggcg ttttctgggt tttgctggtg atcgctccct ttgctgctgt ggtgctgatc
    15961 tttacctcac ttcaatttgg gaaatgggtc tcccgaaatt aaacccgcca atattctccg
    16021 cctccgcctt cgcgcttcat caacccggcg ccgacgagtt cacgtctgag gctggcagta
    16081 tcttcatgat attgggcaag aatctcgttc actttttttt ccgaatagcg tttctctaat
    16141 tcaaatgcct tgaccacgta acgcagaatg gcatcgagtt ttttcttttg ggcggggatg
    16201 gtcttgaggc tgccatcttt gcggacgtaa tctttgacta ctttattgtc gtaggcgtca
    16261 gcatccacgt ttgcaacgga agcggcaaga ttttcctgtg aaaacaggct tttcgatttg
    16321 gtttccagtg ctttttcatc gagttgatag acgttatagt aactatcggc tttggcgctg
    16381 acaaggccga cttgtgcaag ctttgcaaga tggtgtgaga ccgtaggcgg cttgatgttt
    16441 agcagggcgg caagttcttc cacgctgtag ggttgttgcg ccagcaagcc gacgatcttc
    16501 agccgatttg catcggcaag agccttgaag aaggcaacca attcttcgct catgcaactc
    16561 tccaatagcg tgagccgtca ctttcacgat tgagtaaacc tgcttcaata aggtcacggc
    16621 gcaggccagc ggtatctatg tggaagcgcc tgatgatggt gttgacttct ttttcggtat
    16681 agtttgtgcc aaactcaaaa aatccgacca gataatttag aatcgcctgc agttttggct
    16741 gggcagggat ctgtttgatg cttccatcgg cattcaggtg ggctttgagc accttttttg
    16801 acttctcgtc caggtcgggg gcagggataa aaacgggacg ttcttccgcg agtttttcac
    16861 gagctagagt ggcaagcctg tcatcgttta atgtgtagat ttcttcagtt tgcagcaccg
    16921 cgccgacgtg ttccagaaaa gtgaggtgat ttagcgcttc cttgggggag aggttgaggc
    16981 gtgccgtaat ctccgcacga gagagcgggt tttgtgagag aagaccaatg atacgcaacc
    17041 ggtttgggtt ggacatggct tttacaaaat caagcatttc agggttggtt tccatttttt
    17101 atatccgtaa atctaatttg atgactatct aattgaattt tagcgaaggc ttttggtttt
    17161 gtcaactggt aaaaagaaaa ccgccctgtg gaatttttcc acagggcggt ttctactcgt
    17221 tctttgtatc gcccagaact ggcatggccg attcgatctc ttccggtgtc tggcctgcct
    17281 ccagcctgtc caccacttcg ttgaattcag ttggaatgtc ctcacccatt tcgttgccca
    17341 tttttttcat catacgtgca agagccttgg ggtcgtctcc gatctcttcc atgcctgcca
    17401 ggccatcagt ggcggtttcg attcggctgg attctgattt ggcgatccgc actttggtca
    17461 tacggcggcg gacgttttcg ctgttgcagt gcgtgcagtg gacggttttt tttccgtact
    17521 cgctgaaggt ttgaaagact tcaaatcgtt tttcgcattt gttgcagata aaatcataag
    17581 tgggcatgaa tatattttac tgcgaatcag gcccaaggtc tggggagagg cggatatctc
    17641 tttggtacaa tacccccatg cctacttcat ttgaactccg caaacccaat ccactcctca
    17701 ttgtcatctc cggcccctct ggtgtgggca aggattctgt cgtacagcgc atgaaagagc
    17761 gcggttttcc atttcatttt gtagtcacgg ccaccacacg cgataagcgc gaaaatgaaa
    17821 caaatggcag ggattattgg tttgtttcga aggatgaatt tgcgcgcatg atcgatgaaa
    17881 acgaattgat cgaatatgcg atcgtgtatg gtgattacaa gggtattccc aagtgggagg
    17941 tgcgtgaggc gctcgccagc gggcaggatg tagtgatgcg catcgatgtg caaggtgctg
    18001 aaacagtgcg aaaactcgcc cccgaggcgt tgttgatctt tattacctgt gaaggcgaag
    18061 atgaacttga gcggcggctg cgggagcgaa agaccgaaac agcagattcg ctgtctttgc
    18121 gtattgcaac cgctcgtaaa gagttgcagc ggatcgagtc tttcgattat gtgattgtca
    18181 atgaagattt tcatctggat gatacggttg ataaggtgcg ggcgatcatt gctgccgaac
    18241 atttacgggt gcagcaacgc aaggtgactt tatgatcgaa ccccccgctt cgcccccgac
    18301 cgatgtgcct gttccgcaac cccaggcagt gagtgtgcgt ttgccgttca ctacccccac
    18361 agtgacgtat gtccttttgg gtttcactgt actggtgtat gttttgcaat tgttgagtgt
    18421 ggctgtcttt gggtatgcgg tttacgaaat tgactggctg gaagtgtacg gggccaggtt
    18481 taatgaagcg atccgcatgg gtgagttatg gcgttttatc accccggtct ttttgcatgg
    18541 ttcgatggct catatctttt tcaatatgta tgcgctgtta tccattgggt cgtttttaga
    18601 gcggcatttt ggtcatggca ggtttgcctt gttgtatttc cttggggcct ttgctggaaa
    18661 tgtattttct tttttactca ccggtgaaaa tggatattca attggtgcat caactgcggt
    18721 ctttggtttg attgcggcag aggtcatctt tttttaccaa aaccgaaagc tgtttggcgg
    18781 gcaggcccgg caggcgatca gtaatgctgg tttcattatt gcagtcaatt tattcattgg
    18841 ccttgctcca gggatcgata attgggggca tgtagggggc ttgtttggcg gtgcaatctt
    18901 tacctggttt gccggtccgt tttggatggt tgtgggaacc ccgcccgatt tccatttgca
    18961 agaccgtcgt gaaatgcgcg aaatcattgt cggtgcggct gcagtctgcg ttatttttgg
    19021 agcacttggg atttttggga tgttttccta gatgctgctg ctttctgggt tctttttcgt
    19081 tatgaagcta aaaatttcat gcatgaagag ttaaagagaa gaacttgctt aaacaaaaaa
    19141 cgcggccaaa tactggccgc gttttgattt tcaaatgagg gttagcttgt gtgggcaagc
    19201 cgtgggtcga gcgcatcgcg cagaccatca cccagcaggt tgaaggccaa cacggtcaac
    19261 atgatcgcca gaccggggta aaacacaagg tgaggggcgg tgaacacctg attgcgttcc
    19321 gcgccgagca tggaacccca ttctggtgtg gggggttgag cacccaaccc gaggaaggac
    19381 agggcggcag cgtccaaaat ggcggaggcg atgcccaatg tgccctgcac gatcaacggg
    19441 gtcagcgcat tcggtaggat cctcttgaag aggatctcaa aggtgttgcc gccaagcgca
    19501 cgcgtagccg acacataatc catttcacgg acggaaagta cacttccgcg caccacacgg
    19561 gcatacgcag ggatggatac gataccgatg gccagcaagg cgttaatcaa cccaggcccg
    19621 atcacggtga cgatggcaat ggccaaaagc agtgaaggaa atgccagcag cacatccatc
    19681 acacgcatga tgatgttatc gatccaaccg ccaaaatagc cgcccagcgc accgagaacg
    19741 gtgccgatga tgatggcaaa ggtcactgtg gagaaaccaa tcatcaggct cagacgggcg
    19801 ccatacagca ttcggctgaa ttcatcgcgc aggttgccgt ctgtgcccat gatgtgttgg
    19861 ggctgatcgg cagggcatcc caacgcatgg atgcaaggtg aggcgcgctt ttccactttt
    19921 tgaggcgggt ctgcatcaag catggacagg gtcgggtcgt acggggcaag ccattgggcg
    19981 gtcagtgcga tgaaaatcaa cagtccaaga atgaccatgc ctacaatgga tgatttacgg
    20041 cggaataatc tacggcgggt gatctgccag agactgaccg aatcaagcga tagtgcttca
    20101 gcgtccaatt ttttcgggga gacggattgg gttgtcatgg tcagttccta gtccagacgg
    20161 atgcggggat cgagataagc atatgaaaca tccacgatca aatttagaat aacgaagaaa
    20221 attgcaatga cgactgtaaa tccctgcaca atgccgtaat cacgggctgt aattccatca
    20281 tacagaattc taccgacgcc cgataaattg aagacggttt cggtcaacac agcgccaccc
    20341 aacaagccgc ccaaagacaa gccgatgacc gtggccagtg gcagcaaggc attgcgaaat
    20401 gcgtgtttca agatgaccga gcgtctcacc agaccttttg cgcgggcagt acgaatatag
    20461 tcctgaccaa gcacttcgag catggaggaa cgggtcatac gggcgatcaa agccatcggg
    20521 atcgtgctca aagcgaaggc gggcaaaatc aagtggatcg acgcgtcttt gagaacttcc
    20581 caatttcctg aaaggattga attaaaaaca ttcatgcggc tgatgaaatc cgcaagggag
    20641 aatgaaaaag taccttcttg cagttgccac ccccatactt caaaaaatgg agttgtgatc
    20701 aaaccagcgc tcaaacgacc tgaaggcgga agctggaaaa tggtgtcctt caaggtaagc
    20761 gagaagatat aggcaagcat caaccccaac caaaagaccg gcatggatac gccgacattg
    20821 gcccataaca tggttgccac atcagcccat gaattgtgac ggaccgctgc aatgacgccc
    20881 agtggaatgc cgatgatgac actgaaggca agcgctgcga tgctcaattc gaaagtgatc
    20941 ggcagacgtt cggccagcaa ttcggtaatg ggtttggaat agcggaagga atttccgaaa
    21001 tcgccgtgag cgatctcgtt cacgtaaatc ccgaattgga ccaggatcgg cctgtccaag
    21061 ccatggcggt gattgaactc atcacaaacc accttggtgg ctttctcgcc caacattgcc
    21121 cggcagggat cgcccggaat aaggcgcgca agcgcaaatg tggcaaacag aatgccaaat
    21181 aataccggta tgctaagaac gagacgtcga aggatataag tgaccatgat aatttgggga
    21241 gacccccggc atttctgccg ggggtctaat agacttgcct tgattcagat tactgggcgg
    21301 ggggagcgtt gaggaaggtg ccgaagaggc cgctccagta ggagaacgca ccggtgttgc
    21361 cggtgtggag cgggttggcc gcatcccact gaatggagag ggaggtcaca acgtcgttcg
    21421 catcgaggga ggaaccatcg tggaagagga cgccctggcg caggtggaag gtccactcgg
    21481 tcaggtcagc gttgacatcg taggactcag ccaaagcggg ttcaactgcg gtgccgccgg
    21541 tttcgtaagc aagcagagac tgggtgacct gttcgcaagc gcggagtgat tcaccatcgg
    21601 tttcatcagc gcagtagagg gagatcggtt cagcgttctg catccaaacg aaggtgtcgg
    21661 tgccattgtt catgacagag aattcttcgt tgccgagcgg gctggcgaaa gcgccttcaa
    21721 cagaggcctt gaaggccaaa gcggaaccac catgagcaac ggggatcatc ggcacgtgag
    21781 tgcggatggc gttgttggcg gcttcgtaga acggagcgcg ttcagcgtca gaagcgagct
    21841 gggcaccctt atcgagggct tcgacgatgt catcgaactt gtcgccgaac tgcttggaag
    21901 cgccagcgcc gaagtggtag ccgaggaagt tggtctgatc aggatagtca gcaccccaac
    21961 ccagcatgta caggccctgc aactgaccag cgtcagcagc agcgaggaaa gcaccagatt
    22021 ccatcacttc gatgttgagg gtgatgttca ggttttcttt caactgagcc tggaggtcct
    22081 gagccacaac gttcgggtca ggcaggtaac cgcggacaac gtcgcggtag ttcagaacgg
    22141 tttcgaaacc atccggatag ccagcttcgg ccaaaagggc cttcgcagct tcggggtcga
    22201 aggaatacca ttcatcgcct acgcagccgt tcgggatgga gcagggggtg aagtgagagg
    22261 caacttcaga accagcgggg tagaagttat ccacaatgcg ctggcggtca acacccatgg
    22321 cgatcgcctg gcgaactttt tcgttggcca gaggattggt gctgttatcg aagccttcaa
    22381 cctggggatt gttgttgaag ccgatgtaca tgatgttgag ggccgggcgg ttgaagagag
    22441 cgaggttggc atcgccttca acggtggcga aatcatccgg accgacattg tcgataccat
    22501 caacagtgcc agactggagt tcgagcaagc gctgggcaga ttcagtaccc cacttgaaga
    22561 cgagggtctc ggcgccggag aattcacccc aatagttggg gttggccttc aaaaccaact
    22621 ggttgccgcg ctcccaggat tcaaccatgt aggggccggt accgatgggc ttctcaagca
    22681 ggtcgccatt gccgccgctg gcttcgagat attcggcagg ctgaatagcg aaggaagtaa
    22741 aggcgatctt ggacgggaaa gcaggatcgg gcacgcagag cgtgatcttg acggtgaatt
    22801 catcaaccgc ttcgatggac ttgaattcgc caccgtaatc gcagtcgggg gattcaacct
    22861 tcatgccctc ataagccgca ggggcttcag tggcagcggg agcttcggtg gcagcagggg
    22921 cctccgtagc agcaggcgct tcagttgcgg gggcttcagt tgccgcaccg ccgccgcagg
    22981 ccgcaagcat gatgcttgca agcactacaa gcgaaagcaa aacagaaagg tttcgtttca
    23041 cgtttctcct ccttgagaga aataagtgga ttatggagca tgggaaattt tttttcctgc
    23101 acactcctga ctggcaaatt atacaacaaa agtaaactac caaacagaaa acgggcgtcc
    23161 gacaccatac aaccctactg tagaagggtc tgatggccat attccggcga ttttttcccc
    23221 gcatttgggg cattttccat cccccgtgat ctgatacgat tggatgacat acccgcttct
    23281 tctcacaagc tgataactgc actgcggaca atgggtatct tcatactccc caacattgcc
    23341 cggtaaattc cccgcataca cgtatcgcaa ccccgcttcc cgtccaattt cagcggcgcg
    23401 caggagcgta ttgccatctg tattgtctgg ctcggtcatt ttatagtctt tatggaaggc
    23461 cgttacgtgc catggaatat ccaccgaaag cccggcaagg aatcgtgccg cctcccatag
    23521 ctcttcattt gaatcgttga agcccggaac gaccaaagtc accacctcga cccaaagccc
    23581 catttcgcgc gcacgttgga tgccatccag ggtgtgttgc aatgtgccgc ccaattttcg
    23641 gtacccctta tcgctcatgc atttcaagtc tatcttgtat gcatccagat acggggcgat
    23701 gtatttcatc acctcgggcg tattgttccc attggaaacg taggcacaca gcaggccatt
    23761 ggctctggct gccttgaaga tttccaccgc ccactcgctg gtgatcagag gttcattata
    23821 ggaagagacc accaccgacg catttgtgcg ttttgcatgg tccaccattg tttgcggcga
    23881 tacctcgcgg atgaattgcg ccgaaacatc tgacgccggg tcacgcaggg cttgtgaggt
    23941 cagccagttt tgacaatacc cacagtgata gtcacaaccc aacatgccga aggtaagtgc
    24001 attgacgcca ggcaacacat gcgaaaaagg ttttttctca atggggtcac tttgcagagc
    24061 cgccacataa ccatgcggca caaacaattt tccatcccta ttaaatcgta cctgacaaat
    24121 tccacgcctg ccctcgcgga tcaaacaacg atgtccgcag gcgaggcaac gcacatcgcc
    24181 gccttccagt gcttcgtaca atttgccttc aaccgttaaa ccatccagcc gttttgcgag
    24241 tgaactcatt ataatcagcc tatgttacat ggtatcgcat catttgtttg attttagtga
    24301 aaggttgtgg attgatgaaa caagcgataa ctgtcgtgat tcaagcaggt ggtatgtcat
    24361 cgcgcatggg tgaggataag gccctcaaac cctttttagg gcgcccgctc atccaacgcg
    24421 ttattgaccg gcttgcaccg atcgcggatg aagtgattgt aacgacgaat cgtccggatg
    24481 agtatgcctt tctcggtctt cgcctcatcg cggacctcaa gcctggtcgt ggtgccctgg
    24541 gcggactcta caccgcggtt gcatcggcgg ctttttcaca ggtggcggtg gtggcctgcg
    24601 atatgccctt cgccagcgat gtgctcttcg aaggcgcgcg caggcttatg gtcgaagaag
    24661 atgcggatgt tgtcatcgcc aaaacggatg agggctatga accgttccat gccctgtatc
    24721 gccgcgagac ctgcctgcct gccattgagg ctgccatcga ggccgatcaa tggaaggtca
    24781 tctcatggtt ttcaaaggtc aaggttcgtg agttattgcc gcaagaagtg aaggcttttg
    24841 atccttccgg cttgtgcttt tggaacttga atacgcccga agaatttgcc gaagctgaaa
    24901 aacgcgctag cgggtaacca gcaccggaca gggtgcatat gcaacaactt tttggctggt
    24961 gctgcctaat aacaagccag ccagttttcc caatccccgt gaacccatca caatgacatc
    25021 actttgacga gtggccgcca catcaatgat ggcttcagcc gctgagcctt cgatcaattc
    25081 tgtgtggatt tcacacggga tgctgccaat ggtttgaatg gttttccgca ggatggtttg
    25141 ggcttcttcc atgcgggcat taatggcaaa ttgcatgttg ggctcgccaa agtagggagg
    25201 aatcacatcg taggctacga caatacgaag ttcttttgcg gttggattgc gggccagttc
    25261 cgcggcaatc tgggttgcat gcagggcatg ctccgatccg tcggtcgcca aaagaatctt
    25321 tttaaacatg ttgtctcctt gttcgtgggt taaattcagt atacagttcc aatgtctgat
    25381 tttcattaaa gaaaaagatc tgcgccattt gcgcagacca aaataatgaa caccgactat
    25441 ttcccctgta attgggtgaa aagctaggcg ttttccaata tggtgatgaa ttcacccgcg
    25501 gtggatgggc ggctttcggg tttctttgcc attgcccgtt gaatgccgtg agcgacatta
    25561 cgaggcaggt cacggacgat ggtgcgggca tctggaggcg gggcattgag atgggcaagc
    25621 aggagggcgc cggtgctggc atgttcgaac ggcagggtgc cggtcagcat ttggtaggtc
    25681 atgatgccca gcgcgtaaat atcggcgcgg ctatccactt cggcggaggc ttggatctgt
    25741 tcgggggcga tgtaatcaag cgtgccaagc atgttggtgt ttgtcatcag tgtctgcgaa
    25801 tcgacgatct tggcgatgcc gaaatcggtc agcaccaccc gcggcggaat ggtggacgaa
    25861 tccagcatca cgttggaggg tttgatatcc cgatgaacca aaccattctg gtgggcgtaa
    25921 tccaatgcat cggctacacc gcgcaaaatg gagatggtct tggggagggt gatgcggcct
    25981 tcctgtttaa tgaggttgtt gaggtctggc ccggagagat gctccatgac gatgtgatag
    26041 acgccgttct catctccaaa gtccatgatg ttgacgatgt ttgggtgttg caatctggcg
    26101 atgatctccg cctcgcgcat gaaacgcttg cggaatgaat cgtcggaggc cagggcggaa
    26161 cgcagcactt taatggccac gggtttgttc ttggtctggc tggttgcgcg atagacttct
    26221 gccatgccgc cccgcccgat caggcgcagg tcattgtatg aagaaaatct cggcgttgtg
    26281 gaggaagaat cgggagaaag ggtgatatct gatggggttt tctgataatc gatctcgatg
    26341 tgatagaaga agcgatctac aaagcgctgc agtttttttc gggctggctg aaacaatgcc
    26401 cctgctgaaa cggagaagat cgtggccgcc acaagggatt gatctccttg atggaaattc
    26461 ttgaagacca gcgagatcaa cccaagcatc acagcgaagg tcagccccag cgcgagtgtc
    26521 atgctgccat agacaaggga gcggttgatg atgaggtcaa tattgaagag ttttgtctgt
    26581 gcaatggaaa tggaaatgga gagcggaagt accaaaaaaa ggaaaatgct cagcaggcgg
    26641 gaaacaagca gaatagccgt catggtcagg gaagattctg cgggcttcgg cagaaaggat
    26701 agccccacct gaaagatgat gaaccataga aatggcccca aagtgccgat gaatacccag
    26761 cggatctgtt gtttatgggc gagggttgcc ttgcggaaat agcggttgag cagggtgaag
    26821 aaaccgatca gataccagga ggtggtgatc aacaggtagc cctgcccgag tttgtcccaa
    26881 aagaaaggcg cactggggaa gagatagatt cccagcattg tgaccacgag cggaatggcc
    26941 aaaaaacgca gccaacgggg tgtgaatttt ccatccggga agagcattgt gaacaggaca
    27001 atgccaatat cacccatggc cagcagcaat ccgcctgcaa attccgtgcc cggctgcaat
    27061 gcgattgcat ttgccaggtc agtaacgcgt accccgaagg tgatcagaaa gatcgaggtg
    27121 aaaatcgcaa accagtcatc ggaactgtgc atgacgatca tcaccccaat gaccgaaaaa
    27181 ccgagcacca gcagaatatc cacaatgtaa taaaaaccga tttgcagcag ggaggagatc
    27241 ccaagcaggt tttgcgccat ggatgccttc atggcattgc cgaactgcgg cgcaatcgcc
    27301 tcccccagaa catacacaat gtagagaaca accacagacc aaacgccaag caatgcacgg
    27361 cgcaagccgg ggcggctttt tagtgaggtc tcgctggtga cgggatttgt gatcatgttc
    27421 aatcaaccat tgtcgttatc ataacaaaaa atagcccgga taagatttcc cttgcataac
    27481 tgtcaattac ggcaattacg ccgccacagc catgcaaatc tacgctgagg cggcaaaaag
    27541 gtttctcaca cagggatgga ctataataac cgccagtttt cacttcaatt ttggagtctt
    27601 cccatgacga ttgatgtctc tgccatccgc aaacaattcc ccgcattaga ccgccccgcc
    27661 acgttcttcg acaaccccgg cggcactcag atcgcaaaac actcccttga ccgcatcaac
    27721 aggtatttaa tagaaagcaa tgcaaatcac gagggggcct ttgccaccag catcgcctcc
    27781 gatgccgtgc tggatgaagc ccaccgcgcc atggccgatt tctacaatgc ctcttcgccc
    27841 gaagagatcg tctttgggaa caacatgacc accctgaccc tgcacctcag ccgctccatt
    27901 tcgcgtgact ggaaagaggg ggaagagatc gtcgtcaccc gcctcgacca tgatgccaac
    27961 gtttcgccct gggtgctggc ggctcaagac cgcggggtga aggtcaattg ggtggatttc
    28021 gacgtagaag atggcacatt gaaactggac cagcttcagg cggcgcttga acgaaaaccc
    28081 cgtctgctgg cggttgggta tgcgtcgaat tcccttggca cgatcaaccc tgttcagaag
    28141 atcatccaaa tggctcacga tgccggcaca caggtgtatg tggatgccgt gcaatacgca
    28201 ccgcacggcc ccatcgatgt gcaaaggctc gattgcgatt ttcttgtttc atctgcttat
    28261 aaattcttcg gtcctcatgc gggcattttg tttggtaaac atgaactgct tgaaaaatta
    28321 tttgcctaca aagtgcgccc agccaccaat aagctccccg gtaaactcga aacagggacg
    28381 caaaaccatg aaggcattgc aggtgttctt ggtgccattg aatatttcga atgggttggg
    28441 cgtgaatttg gcggcgaaca tgccgaaagt tttgcaggca gcggatacac cgggcggacc
    28501 ctgaccttga aaaaagccat gaccgccatc cgtgcctatg aacatgaaat gaaccgcgcg
    28561 cttctttcca cactggaagc tgttcccggt ataacgatct ttggtctcac cgatacacgc
    28621 cgcctcgatg aacgtgtggc aaccttctca tttcgtttga agaatctcca cccgcgcaaa
    28681 accgcggaat tgctcgccga aaaagggatc tatgtttggg atggcaacta ctatgccatt
    28741 aacgtcagcg aacggctcgg ctttgaaggt aatggcggca tggtacgggt tggcgctgcc
    28801 cattacaaca ccctggatga aatcgcccgc cttggagacg tattgaagga aatcgcggaa
    28861 aaagccgcct aaggtgcgca tttcgtaaac aaggtgctga tggtttttca aattgtcctt
    28921 acgaccgttc ttgcgctggt cttttctttt gtcattgcgc cgttgagcat ttctcttgca
    28981 tggcgttttc atctgatcga tgaccccggt ctcgctgccc gcaagatcca caaagccccc
    29041 atgccacgtg caggtggcac gatcattttt ctggcgattt tggcaggcgg agcattgagc
    29101 ggggttctca cgacccgccc atttacttcc atcatccttg cttcgatgat cgtctttgcc
    29161 tttggcattt gggatgatat ccacaacatc cgcgccattt ggaaattgct cgggcaaatc
    29221 cttgccacgc tgattttgat ctggcaggga gtggtggtgc ttttccccgg aaacacggct
    29281 ttgaacatag ccatcacact gttctgggtg atcggcatta ccaatgcctt caacctcgtt
    29341 gatagcatgg atgggttggc ggttgggctg gcgggcatca ccgtcagctt tttgatgatc
    29401 ggcgcgctgt atgcggggca gaatacactg gctttgtttt gtgcggttct tttaggggca
    29461 tgcatcagcg tcttccatct taatttttca ctctcccgtc tgtttctggg cgattctggt
    29521 acgcagctgc ttggctttac ccttgccgcc attgccattg tgtacacacc gccgcacctg
    29581 cctcaagcct cttcctggtt cgtgcccatt ttgctgttcg gaatccccat ttttgacacg
    29641 accctggtca ccttttcgcg gatgcgcaaa aagatgccgg tgtataaagg caaccgtgac
    29701 cacaccttcc accgcctcgt ggatctgggg gcaacgccgc accgtgccgt gctttcaata
    29761 cacatggttg ccatcctgct cgattgtctg gccttctttg ctttatccct ctcccctttg
    29821 tgggcaaaca tcacatttgg ggtcattctg ttatccggtc tgttcgccat tctctggctg
    29881 gaagcaaatc aaaaagtgga aaccctctaa aaaggagatt gaacgatgac agttgaagga
    29941 actcatggtt tatggggtga cctgcatcgc cctgacctcg accccaaaca agccgatttc
    30001 agtgtggtgg gcattccctt tgacgggctt gccagtgcgc gcaaaggtgc cgcccttgcc
    30061 ccggaacgcc tgcgctactg gtcacggcat ctgacgccat tcagcgaaga ccgcacccgc
    30121 ctgaaagata tcaccctgtg tgacctcggc gatatttcca tcgtcaaccc tgcaaccgat
    30181 ttcaatatga ttcgtcagcg ggtggctaca ttgccaaata ttccgatcat gttgggcggc
    30241 gatcattctg tttccattcc gatctttcaa ggacaacgtg agcgttatca ggataaacgt
    30301 cttggcgtgt tgtggatcga tgcccatccc gacctgtgtg ataccttcga cggttcgaaa
    30361 ctctcccatg cctgtgtgct gcgccgcgga atcgaattcg gcattgagcc gcacgatatt
    30421 tgtatggtgg gcctgcgctc atgggaagat caggagatcg atatcgtcga aagcggcggc
    30481 gtgcatgtgt acactgccgc cgatgtcgct gaacgcggca ttcgcaaggt ggctgacagc
    30541 gtccgcaata ttctcaacga ttgtgatgcc atccatgtct cgctcgacat tgactgcctc
    30601 gacccatctg ccgcgcccgg tacgggcatc cctgaatttg gcggcatgac ctcacgggat
    30661 gtgcttaccc tgctcaagtc catgcaaggg cttccgctgg tggggttgga tgtggttgaa
    30721 attgcccctc cgcttgaccc atccgaagcc actgtttttg cgggtctgaa gatcatcatg
    30781 gaattcatcg ctgtgatcgc gcgcgaaaaa caaaaaggct tgtgaaatgc cagccaattc
    30841 attgaacgtg acgcacaaca cggataagaa ccgttttgaa gtccaaatcg attcgctgac
    30901 cgctgaattg gtgtaccgct tgaatgggaa tgtcatcctg tttctacaca cgggtgtgcc
    30961 gcccgcgctc gaaggcaggg gcatcggcag cctgcttgta aaaacgggct tgcaatacgc
    31021 aagagaaaag aatctcaaag tgcagtcttt gtgctggttt gtcagcggct acattgaacg
    31081 ccatcctgag tttcaagacc ttgttgggta acagagcaat ccacgaaatg taaaactgaa
    31141 gccatctgca aagatggctt cagtttattc ttcgatgttc attttctccc tgatcaattc
    31201 catcacgaat tttcctgcga gcggaactgc ctctgcaaca ggcggtgaaa gttcctcact
    31261 gaattcgtga atgcgttttg tcgcaatgcc caccaccatc acctcatcag gcagccacgc
    31321 gcccattgtt tttcccagtg cgattgcagt ttgcagcgaa gtatcgtgtg ggctggtggt
    31381 atggtacgcg gaataatttg gaagatcatc cagcttcaat atcaaaatgg atccgatcgg
    31441 ttcatccaat gcaaaagaat ctacaaggat cacacgcgaa tagccgatca agtgctccat
    31501 taaactgatt ccgcccaacg aaagacattc cacatccaca tccagacccg cttcagagag
    31561 ttgcgctcgc acctcttccg cgaccttcca gcctacgcca tcatcgccaa gaatgggatt
    31621 ccc
//
LOCUS       DAOQLC010000042        30854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_469_length_30854_cov_22.155947, whole genome shotgun sequence.
ACCESSION   DAOQLC010000042 DAOQLC010000000
VERSION     DAOQLC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 30854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_30854_cov_22.155947"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1377
                     /locus_tag="PL000_09355"
     CDS             73..1377
                     /locus_tag="PL000_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89149.1"
                     /translation="MSEYSERPSLLQTYTKSFSPLKNRNLRIYLGGQAISLLGTWMQS
                     TAQSWVVWELTRSESSLGIVVMLNTLPLFLLAPWAGGIADRFNRRMILLVTQGIAMVL
                     AFALAFLIQTGLVQIWHIYTFAFILGIVNALDFPAQQAFIGDLSGMGQVRQAITLNIM
                     GLQVARMLGPAIAGVLLKVVGSAAAFWINGVSFMVVLLSIFMVRSQQASHNRPSARGQ
                     FRETIEFIRSQPRMVDLMVFATLVTFFGLSVFNILPSVADHVLNGDSQTYGLLFASSG
                     AGALISTLVLLPLSQAAKRIGLIVSGAAAWMGTFFFLLSNSTWLPLSMAVIFCISLGA
                     PLVLTTGLGVLQLMSPPDMRARIISLFTMLTFGLQPISSIVIGYSAEHFGTQNAILIN
                     SLCLFAGAALMFFFRDGLRQWELKPAVEPILSSKETSQPASH"
     gene            complement(1459..2130)
                     /locus_tag="PL000_09360"
     CDS             complement(1459..2130)
                     /locus_tag="PL000_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89150.1"
                     /translation="MNPIEFISNLFAINSLQFWWYLTRAAGLMGYFLIWLSTFWGLVV
                     SSKILDPILHRTFTFDFHEYLSLIGLGFTAVHVIVLLADNYLPFSLWQILIPFLSPYR
                     PFWVGIGIIAFYFTLLVTVTYYFRTQIGLETFRKLHYLSLAAYLGTTLHGLYAGTDSA
                     LPITNLLYIGTALVTLFVLAYWLVMLYLRRQEETQLKKERALAALRSKTQSRLGSARS
                     TKTTH"
     gene            complement(2117..3052)
                     /locus_tag="PL000_09365"
     CDS             complement(2117..3052)
                     /locus_tag="PL000_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC89151.1"
                     /translation="MQADRFRAMNSEIILLAEGDIPQVVLGFIAAREFIETNEKRFTR
                     FSDASELSQLNRSAGTWFNASHEMFEVVKLALEYHQKTSGLFDPSILPNLRAAGYNRS
                     MDEIRRFGTDLQPATQPQTFDSAFASLQLNESRQAILLPPNMQIDLGGIAKGWIAEQA
                     ARLIRQYTSTCAVSAGGDIFLIGYPEGQDVWEIGLEDPRSPAENISVLYAQEGAVATS
                     SVVKRSWKQGEHTRHHLIDPRSGEPANTNWLSVTVFAKHAVTAEAFAKAFLLADETHI
                     RLFEEQNPELTVLAVDASGNLVSLINPERHLNESN"
     gene            complement(3053..3295)
                     /locus_tag="PL000_09370"
     CDS             complement(3053..3295)
                     /locus_tag="PL000_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89152.1"
                     /translation="MKIWKTAIRVWFTLVSLISFLVGWIVLAHSPKPNQFKASDIPAM
                     PKLKPVPSLEEAMFSGRAESLIQINPRTVFLRTSGS"
     gene            complement(3309..3674)
                     /locus_tag="PL000_09375"
     CDS             complement(3309..3674)
                     /locus_tag="PL000_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89153.1"
                     /translation="MKKHYTALLAAFLITLCIGAGMALVSGSAMLNQKGLPIADTPAE
                     ATATAEYISAEQAQIAQLQKLVAEYQARELTYQNELADAGQQLEQAKVQIQQYEMILL
                     ALQSRGIISIDPNGRVVLQ"
     gene            3813..4484
                     /locus_tag="PL000_09380"
     CDS             3813..4484
                     /locus_tag="PL000_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89154.1"
                     /translation="MRILLVEDNKRLSDSLRLSLMDKGYAVDTAYDGQEGEDLAEFTP
                     YDVIILDVMLPKKDGVEVCRSLRGKRVQSPILMLTARDALEDRVLGLDSGADDYLVKP
                     FEMDELLARLRALLRRESEDKSGLLAIADLVLDPATHFVKRSGQSIDLTSREYELLEY
                     LMRNPNRLITRQMAENHLWSYDATVQSNVVDVYIRRLRRKVDDPFETKLIETVRGSGY
                     RLRRP"
     gene            4504..5958
                     /locus_tag="PL000_09385"
     CDS             4504..5958
                     /locus_tag="PL000_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89155.1"
                     /translation="MVNSLSLLFHSIRFRLALWFVLILALLLAVFSGLLFYMQIQELR
                     AETLNRLQGKLERIESVYVEGGVDPIVLGSLNGGEAPLDRSDILVYFSPNGSVLKMWG
                     PLSKAPVLNLASVEVEEHEPADETITLEGDDGRVEYVFKIIPLFVGQDHTGFMALGTP
                     LDPRDRLGKLLFTLIVLNGFTLVFALAGGFWLADRAMRPVKTIAAKARSISETNLGQR
                     FNLPQKDEIGQLAGTFDSMLARLESAFARQRQFTADASHELRTPLTIVNLETTRALSA
                     QRSVKEYQRVLQVIHSENELMSHLVNDLLTLARMDAGREALHREVLDLSDIALEVVER
                     LEPLAARQQVHLFTGELPEVTVLGDRSSLTQMLTNLVENAIKYTAQNGPDLERHVHVE
                     AGTHPAQAQGWVRVSDTGPGISSEHLTHLFDRFYRVDKSRTREEDDEVRPTGSGLGLA
                     IVDGITRLHNGSIEVQSELGKGSTFEVVFPLHLT"
     gene            complement(5988..6791)
                     /gene="yqeB"
                     /locus_tag="PL000_09390"
     CDS             complement(5988..6791)
                     /gene="yqeB"
                     /locus_tag="PL000_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HNC89156.1"
                     /translation="MTHLIVLRGGGDLASGVALRLHRTGFQVVILELEKPLAVRRAVS
                     FSEAVYEGTHTVEGVTARLVSADQFQVTLEAGEIPVLVDPHANILRNQFLISPQFTFV
                     VDARLLKTAPDPLEVNVPLHIGLGPGFSAGENCHAVIETRRSHTLGRVYWSGATQPDS
                     GQPDGDPRRVLRAPQTGILTAYAEIGDLLQAGQLVADIGEEKITSPFDGVLRGIIRPD
                     IEVLEGMKIGDVDPRSDTSACWLVSDKALSVGGGVLEAVLIKLNEINQN"
     gene            complement(6788..7498)
                     /locus_tag="PL000_09395"
     CDS             complement(6788..7498)
                     /locus_tag="PL000_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNC89157.1"
                     /translation="MKKIYEHIHPIKFRLTVIALLCGATLLSILLFRMRTLLSGSLDY
                     VFLLWNIFLAWIPLGFAHVATHFAWKRRYIVLSVPVAATLWLLFFPNAPYILTDLQHL
                     GSPKPGVPVWFDVLLIIWFAWTGLLLGMVSLFLMQDIVRREFGGIAGWFFVFAVGFLS
                     SLGIYIGRFLRWNSWDMFFNPLGRLTELLYYAAHPSMQSIIFISVFATFFIFFYTTIY
                     AFGRLLQEQAPKTSQETL"
     gene            complement(7554..8252)
                     /locus_tag="PL000_09400"
     CDS             complement(7554..8252)
                     /locus_tag="PL000_09400"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HNC89158.1"
                     /translation="MKNFLLRNRYKIAIFGLMTVASAICVFLVAARIAYSDSIRYTNL
                     VWNLFLAWIPFVLAYLAYTLSWKKWLLYFVIPVTTFLWLIFFPNAPYILTDLQHLAKE
                     STSAPLWYDVIVMVWFSWTGLLLGLVSLYLMHYIIQRMFGRAAGWAFVFIVSGLSSFG
                     VYLGRFVRFNSWDLLNDPKEIVVTILGLAIDPSMRVIAFTILFAVFYLFVYLTLYSFA
                     HLVREDPVAALASH"
     gene            complement(8258..8752)
                     /locus_tag="PL000_09405"
     CDS             complement(8258..8752)
                     /locus_tag="PL000_09405"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89159.1"
                     /translation="MNKQDIQLLYRYNTWANARILDAVARVSAEQYLAADSYPHGGLH
                     GTLTHTLMAEWIWRQRWEGNSPTYFLKPEDFPTFEALRTRWQEEDRLLSAFLEALTDE
                     KLNAPFQFKTTRGVEMENILWQAMTHVVNHGTQHRSEAAAILTELGFSPGDVDLIVFL
                     RELK"
     gene            complement(8749..9624)
                     /locus_tag="PL000_09410"
     CDS             complement(8749..9624)
                     /locus_tag="PL000_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC89160.1"
                     /translation="MPFPYLDENRDLNESARQEAGGSFIKLTDGTTHFELVGPETAPA
                     IVLVHGFSVPYFIFDPTFDFLTKAGFRVLRYDLFGRGTSDRPQTAYNIHLFVRQLKEL
                     LAALNLPRVHLLGLSMGGPITAAFMDENPHSVEKHILIDPAGAKRVRLSLLLEAVKLP
                     IFGELALGLFGSVSLVKGIASDLFDPDLVEHFQARYKIQMQYKGFKQAILSTMRNQML
                     ESFFSTYERVGKLQKNTLLIWGKNDTTVPFENSMEILKAIPHAEFHAIEACGHIPHYE
                     KPEIVNKILMEFLEK"
     gene            complement(9673..11862)
                     /locus_tag="PL000_09415"
     CDS             complement(9673..11862)
                     /locus_tag="PL000_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNC89161.1"
                     /translation="MTNQSVGKSLPRIDAHDKVTGKALYSGDLVKDGMLHMKILFAER
                     PHARVKNINTGKADAAPGVVAVYTSKDVPVNEYGLQIPDQPVLCGPSKKPHTDIVRFV
                     GDQIAVVVARTEVEADAAIKLIQVEYEDLPILSDPKAALRPDAPILHPDRGDTNICVH
                     YKIRKGDMDAAFANADVIVEGEYHTPVQEHAYLQPEAGLSYIDDDGLITVEAGGQWTH
                     ADRETIAHSLGLPNEMIRIIYPAIGGAFGGREDLSVQIVLALATWKLQRPVKIIWSRR
                     ESMIGHGKRHAATVKAKWGATKDGKLIAAEMEMIGDGGAYMYTSNKVLGNAAITSTGP
                     YFIPNVKVDVYGVYTNNVPGAAFRGFGAPQALFMAESQMNKLAAHLGIDPVEFRLKNA
                     LRDGDAMGVGTPAPGPVSIVQCIEAARDQFGWKKSKAKKAAGKKSSPLVRGRGFAAGF
                     KNVGFSFGYQENCWAKVEIHGNGKMERAIVHHAGADVGQGMHTVMVQMAAHVLGLPMD
                     KVEIRTSDSAKQGNPGSASASRLTFMAGNSVKGAAEAALEKWKNEERPAVAEFKYLAP
                     RTTPFNKETGYSTPNFSYAYVAQAAEVEVDTETGHVRVLRLTSADDVGKAINPDLVVG
                     QIEGAVVQAHGYAVMEDYKTKDGRVLTDQFSTYLIPTILDIPEKVDSVLVEVPDPNGP
                     WGARGLGELPFLPVAPAIAAAIHDATGVWISEFPFTPERVLRALGKI"
     gene            complement(11869..12294)
                     /locus_tag="PL000_09420"
     CDS             complement(11869..12294)
                     /locus_tag="PL000_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89162.1"
                     /translation="MGKYNNNTTLKPSSIQSKGTHEIWRGFGCLMILIIPAISIASAY
                     ELIQYGIANKWPIPYELRGKVYLPEIFYSTPGLATIFSPISEVPNLLAIAAASLVLMM
                     LIGGVISVIYAAIYRAIGPSRYGPTDAPPSGIKVKKYKR"
     gene            complement(12313..13719)
                     /locus_tag="PL000_09425"
     CDS             complement(12313..13719)
                     /locus_tag="PL000_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNC89163.1"
                     /translation="MWHEYINATSIEDVLKVLGEKKERARVVAGGTDLILELERGVRK
                     GIETLIDVSRIPGIDQITIDEDNVIHLGPRVTHNDCVASKVIRKHAYPLARAAWEVGA
                     PQIRNRGTVAGNLITASPANDTITPLMALGASVTLLSTRGSRTLPLKEFYTGVRKNVM
                     QPDEMLVDISFPAMKKNQRGTFIKLALRRAQAISLVNVAIIIDLKVDTVKSAVITLGA
                     VTPIITHAEDAEKYLAGKKLTEKNITHAAELATQAAHPIDDVRGSAAYRREMVKIITA
                     RGLRAIRDENEQAGMPKKPILLASTDVAKHPLQATSEFPTAPIETTINGKKYSFKQGH
                     DKTLLRLLREDGMLTGTKEGCAEGECGACTVFLDGKAVMACLVPAPRAHGAEIITVEG
                     LAKEEGTLHPIQETFIENGAVQCGYCTPGFLMSGAKLLEEKPNPTRNEIEQAITGNLC
                     RCTGYYKIVKAMEDASKL"
     gene            complement(13765..14289)
                     /locus_tag="PL000_09430"
     CDS             complement(13765..14289)
                     /locus_tag="PL000_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89164.1"
                     /translation="MSDFFEKVSSQIDPFKKLLSYIPGFKGYVERQTRRDADKELRDV
                     VARRFEEYWSRASNMQAELVSNGMLSYVDDMEKATLSMRTFIDKISRAARGYSGMFDA
                     IKINEKELEAIYQFDVAFFTLGEQVSSALDNVEASLNDESALPAAIRNLTTLSRLAVE
                     TFERRSEVVTGSGN"
     gene            14402..15820
                     /locus_tag="PL000_09435"
     CDS             14402..15820
                     /locus_tag="PL000_09435"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNC89165.1"
                     /translation="MSEHVKELLELVENSLAVDGAAVKVVDAAKFRKNVGKLVERSAL
                     GSDSTVGWSRYLVRAAALELGVYPASIHELYLARGRGEAPMTFTTPAFNLRALSYDAA
                     RAMFRAANKINAGAFIFELARSEMSYTAQRPSEYATNILAAAVAEGFVGPVFIQGDHF
                     QVSAKKYSADAEGELKAVRDLSIEAMNAGFFNIDVDTSTLVDIHLPTVPEQQKLNCEL
                     SAQLSAFIRENEPKGVTISIGGEIGEVGTNNSTEPELRAYMDGYNLEMKKLAPGKPGL
                     SKISVLTGTSHGGTVLADGSIAEVTIAFDVLRDLSRVARKDYGMGGTVQHGASTVAEE
                     NFNKFVQNEAIEVHLATNFATMFFDNIPADLKKEMYAWLDVNSAGDRKPGMTDEQFYY
                     KTRKNAIGPFKAKSYALSEAAKAKLGAAWEAQFDKLFNLLGMKDTKQYVEKYIPNVKV
                     APVLAHYVKEDVAAEDVSDLSD"
     gene            15849..16346
                     /locus_tag="PL000_09440"
     CDS             15849..16346
                     /locus_tag="PL000_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC89166.1"
                     /translation="MPKSDQGVTKDRYMLIPRTAIFLRRGDEYLLIKGAPTKRLWAGK
                     YNGLGGHVEQGEDVFTSARRELLEEAGLTADLWLCGTVIVDAGETGICLFVFCGENAQ
                     GEIKASAEGAVEWVRFDAVSHLPVVEDLPILLGKIHSMKHGDAPFSARSHYDENERLQ
                     VVFGV"
     gene            complement(16385..17152)
                     /locus_tag="PL000_09445"
     CDS             complement(16385..17152)
                     /locus_tag="PL000_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89167.1"
                     /translation="MYHTSKGVFIFFISIVLILTACSGGTESQSQQSGSSDSISGTCD
                     NPLLPVKQGAMWVYVSNGGPGGSFTYTDTVTSVLDGKFTIASQFADLIRTQEWACQSD
                     GLKALQLGGGTAAGISTLGMTAAFTTIEINGISLPKEVTPGMQWAYSLKMQGTMAMPG
                     DQQAQSSGVYSVTTQEIGRETITVPAGTFEAVKLQSNSTIDIVTSFEGIDVPIKFNGT
                     TISWYVPGVGFVKSVENGDFGGTTFAATTELQSYNIP"
     gene            complement(17229..18395)
                     /locus_tag="PL000_09450"
     CDS             complement(17229..18395)
                     /locus_tag="PL000_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89168.1"
                     /translation="MTNTRTPAKIIPRAFPGIKPEEVQEIIINSRIQSYPPETIICRE
                     NEVENTFYMVLEGDFEVSKVINNTEIRILKALTAGDFFGEMALIHNAPRAATVKTKTN
                     VIVLELNKEGFDRVLKHSPSVAMAMVSEISNRLRQNDQLAIDDLRLRAAELADAYQKL
                     AEQELMRREFLSNIAHELRTPLQVASGYLQAMKKGMITSDQFNVAVDTIARNVDQITT
                     LANDLLFLQEIELVLPEFQSVNLVEVAMDVIKRYESKAQSRGVTIKVKGERGISNVRG
                     DAQSISRAVTALLDNAIKFSAPKSEVEVRFSEQRERVLISVEDRGIGIAPEIRPRIFD
                     RFFHLEKSGDELYSGLGIGLSITRQVILQHNGMLDVESQPGKGSKFTISLLKWQ"
     gene            18600..19571
                     /locus_tag="PL000_09455"
     CDS             18600..19571
                     /locus_tag="PL000_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC89169.1"
                     /translation="MTLLASIFAGACQPALTESATVTPLPTFTPTPYYTETPTPTSTP
                     QPTPTPPRLVPNFTHIVIIIFENKEFGTVIGNNQMIYFNLLASSYTLLTEHYAVTHPS
                     LPNYLSLVSGDTFGITDNCEDCYINFLSLPDLIEQSGRTWKTYQDDMPEPCFSGSTLR
                     YVQKHNPFMYFDSIRLNPERCNRSVVPLTQLDEDIAANALPNFIFITPDICYSAHDCE
                     LELADGWLKENVQKLYLALEATKEPYLIILTWDEGQGTHSCCGLPELAGGRVATVLIS
                     PQVRQSFQDHTPYTHYSILKTISEAWALPFLGHAADANTAIIASPWK"
     gene            complement(19577..20140)
                     /gene="rsmD"
                     /locus_tag="PL000_09460"
     CDS             complement(19577..20140)
                     /gene="rsmD"
                     /locus_tag="PL000_09460"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNC89170.1"
                     /translation="MSLRVIAGTARGRKLRSVPGDTTRPVMDRVKEALFNILADDVID
                     SNWWDLFGGTGAIGIEALSRGAAFVRFSDLNRAPIETIRENVTHCGFLQQSEIRRGDA
                     FSMLSGRADKSFEYIYIAPPQYKEMWLEALKLVDAHMDWLTDDGTVIVQIDPTEYQAV
                     ELENLEETEQRKYGSTLLVFYDHRIGE"
     gene            complement(20137..21111)
                     /locus_tag="PL000_09465"
     CDS             complement(20137..21111)
                     /locus_tag="PL000_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNC89171.1"
                     /translation="MTTHRERIQASIEGKKTDRTPIALWRHFPVDDQNPETLAASTLH
                     FQQTYDFDIVKVTPASSFAVKDWGVEDKWMGDSEGSRRYTKRVIHKPEDWEKLTVLDP
                     TSPHLAGQLTCLRLIRENLGPDTPLIQTIFNPLSQAKNLASNDTLVAHIRQHPEAVMK
                     GLETIARTTAKFIEAAAEIGIDGIFYAVQHAQANLLSVDEYTTFGLPYDQQALFPVKN
                     LWCNMLHLHGKDVYFSLLRLLNFQIVNWHDRETHPSLAEAHSLFKGVLCGGLRQDTLV
                     LEDQAKIKEEAADAIQQTKSHRFILGTGCVVPITASHGNLLVARKSVE"
     gene            complement(21108..22667)
                     /locus_tag="PL000_09470"
     CDS             complement(21108..22667)
                     /locus_tag="PL000_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89172.1"
                     /translation="MPKVRQYPILILLIINIVIGLLTFRNYGLSWDEPLFYDYAHSLG
                     YAYSPKEWFSGNFDLENAFGSSGSDHANRGPAYILLAQPVVSVLESFKLDNASAWHLV
                     NFLTFQFGVYLFYRLSKRWVKESAALAATALFAWQPLLWGHAFINPKDPPFLVFFLGA
                     VCFGLEMVDALHTSQTTKFTLWRSTLLAAFFLGIATSIRVLGPLAGLLVCIYAFMQFG
                     QKGIPVLVKQLALYSALAILTAFITWPYLWGNPIQKFIEAFGFMSDNPTQLQVLFNGQ
                     LYRADELPRRYLPTLLVLTLTEPTWFLFAAGLLTALWHQVNITTRKITVLSSFILPTL
                     WFLIPFLYVILRKPPMYDGYRHFLFMLPPVFIFTGFAFEKLFEIIKSRQIAVAVAALL
                     IAPAIYSGVQLHPYQYAYYNLFTGGTSGAFRTFETDYWLTCYKDAVEQFDQQNAQLFV
                     KREPIMASYYASENLNIRDYRAEFGEIRSGDFVLVNTRANEDIKTFKEAPVVLQIRRG
                     DAVFCEIKKIP"
     gene            complement(22675..23148)
                     /locus_tag="PL000_09475"
     CDS             complement(22675..23148)
                     /locus_tag="PL000_09475"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC89173.1"
                     /translation="MTETLTPKSARHSRVTLSQLMHPEHANLLGNVHGGWIMKLVDEA
                     GALACMRHAQKKVVTVAVDSMVFREPIKIGDLIILNAEVTYTGRTSMEAEVQVIAENP
                     VSGERTHTNTAYLVYVALDDGGHPTTIPPLAIESEEEKQRMEQAQKRQAHRISQK"
     gene            complement(23157..23537)
                     /locus_tag="PL000_09480"
     CDS             complement(23157..23537)
                     /locus_tag="PL000_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89174.1"
                     /translation="MSTQKPVLIPIYTSKGDAEAFLLYPYLFNRLGDWIGFVNQKREV
                     YSVLGYFVGTLTNDPRIIGKRATSTLKPRQQPPPKPEKVFPPATVPLARMMPDLGPSA
                     VDILLDAPERLHTLDAGEQRQDLD"
     gene            complement(23540..24007)
                     /locus_tag="PL000_09485"
     CDS             complement(23540..24007)
                     /locus_tag="PL000_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNC89175.1"
                     /translation="MILTNAKERTRFLKFAMVGTIGAVIDFGVMNLLSHFTDMSLVYA
                     GTISFICAVVSNFIWNRFWTYPESRSRPLINQLGMFFLVNVAGVVIRIPTLHYLEPPL
                     LHFFEGVFHASYATAEFYAKNITLASAVGIVMIWNFFVNRYWTYNDIDHSSEA"
     gene            complement(24004..24960)
                     /locus_tag="PL000_09490"
     CDS             complement(24004..24960)
                     /locus_tag="PL000_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC89176.1"
                     /translation="MRILITGAAGFLGSHLCDHLLKDGHEVIGMDNFVTGSPDNIAHL
                     ADNDHFSFYRRDVSEYIFVPGKVDAILHFASPASPNPQSKSGYFNLPIQTMKAGALGT
                     HNCLGLARAQNARFLLASTSEIYGDPEVHPQVESYTGNVDPIGTRAVYDEAKRFAESL
                     TMAYHRSHNVNTSIVRIFNTYGPRLDLEDGRALPNMIKQALLGQPLTVYGDGSQTRSF
                     CFVDDLIDGIVKLLYSEEHFPVNMGNPHERTILQFAETINKVTGNTAGIVFVDARSAR
                     DPQRRRPDITRAQTILNWEPKVSLEEGLIRSIPYFKQKLGLA"
     gene            complement(24984..26570)
                     /locus_tag="PL000_09495"
     CDS             complement(24984..26570)
                     /locus_tag="PL000_09495"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HNC89177.1"
                     /translation="MPRLRLRPHLLLFLLISIWLTGSCRSPQVGEQITVTINADGNAA
                     QVSVTAGSTVSQALQSAGIIPAKLDRSEPPLYTVLSNGDSITLTRVEEVFETEQVTVP
                     FERQIVRNETLPEGETRLVQAGVNGLLEVTYRRILENQVEISKTSVKTVVLTEALPEI
                     VMVGAQSSFTPLNIPGTLVYLAGGNAWIMEGSTANRRIIVSTGDLDGRIFKLSPNGEY
                     LIFTRKSKKPASQEINTLWAVRTKNIEPKPIWLQAYNVVHFAEWIPGTNSVAYSTVEP
                     RSTAPGWQANNDLYRVSLTGGSPRKMLEANSGGVYGWWGMSFAYSEDGRLAYARPDGI
                     GLVDQDGGYLKPMLGITPLNTHSDWAWIPPITWGSDGKTLYYVTHAPAPAPIVGEESP
                     FFDVAAISFENGASVSLIDAAGMFSYPAVSPSRWDGRQKDYQVAYLQSIFIEQSETSR
                     YRLMVMDRDGSNSRALFPPADTNGIEPQTPSWAPEPLEGQVGDFIAVTYQGNLWLIDS
                     GSGKAYQVTGDGLIVGIDWK"
     gene            complement(26588..27160)
                     /locus_tag="PL000_09500"
     CDS             complement(26588..27160)
                     /locus_tag="PL000_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89178.1"
                     /translation="MKTKIYHLTFLFAVLMLASLACNIFVGGPDYPTQTVPVSADEVQ
                     TMYTQIEQAFTAGAETGVVTLQITENQLTSLIALKLQEQTDPPFTDPQVLLRNGQLQM
                     YGKLASGSFNANMLITASVGIDETTGMPKIEVSSVDLGPIPAPEGLNAAINAMIAEAF
                     TGSLGPAATGFRLEAITIADGVMTMTGRIK"
     gene            27206..27787
                     /gene="scpB"
                     /locus_tag="PL000_09505"
     CDS             27206..27787
                     /gene="scpB"
                     /locus_tag="PL000_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNC89179.1"
                     /translation="MSDSQTTSASVSTPEQSLAAKIEAMLFVSAEPVPMAQLAAALDV
                     TASVVERGLKELDEALLNRGLRLQRNGGRVQLTTAPEMAVLIETFLGLEATTHLSRAA
                     LETLAIIAYQQPCTRPQVDAVRGVNSDGMLKSLLSKGLVQEAGRTDGPGRPILYSTTP
                     EFLQHFGLGSILELPPLSAPTEPDNEHSELLKG"
     gene            complement(27859..30177)
                     /locus_tag="PL000_09510"
     CDS             complement(27859..30177)
                     /locus_tag="PL000_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HNC89180.1"
                     /translation="MAKDIKSIISKMTLEEKAALCTGASNWSTTPVERLGIPEMIVSD
                     GPHGVRRVADVRSMGQKSLPATCFPTASCTASTWDVDLIKQMGIALAEECIALNVDVL
                     LGPGVNMKRSPLNGRNFEYFSEDPFLAGELAANFINGIQSKGVGTSLKHYAANNQEFQ
                     RFSISAEIDERTLREIYLPAFEKAVKQAQPWTVMCSYNKVNGTFASEHHQLLTEILKE
                     EWGFEGLVVSDWGAVRNRVSALKAGLDWEMPGPQDLRVKAVVEAVRSGDLSESTLDES
                     VRRILRIVFKAKETAKGNGTFDVDAHHELASKIATEGVVLLKNDGLLPLKDHQHIAVI
                     GRSAETAHFQGGGSSHINPTKVAMPYKELQARAENAELTYAEGYPTDNSFQQGMIDDA
                     VKLAQSADVALLYIALPTFKESEGYDRHDLDLTLQQIALIKAVSKAQPKTVVVLNNGA
                     PVAMSEWIDDVSAVLEGWMMGQAGGAALADILFGRVNPSGKLAETFPLKLTDTPSHIN
                     WPGGAGKVHYGEGIFIGYRYYDAKEIPVLFPFGHGLSYTTFEYSNAKVSSNNFKDVDG
                     LTISVDVKNTGKLAGKETVQVYVHDQKSDLIRPVKELKGFAKVELQPGETKTVSIHLD
                     FRAFAYYHPEHKQWITEDGGFDILIGASSADIRSRLTTTLQSTLDLPCILDIESTLRE
                     WMADRRGRQVLGSLYEQIENQVRRLFAGEDRYGNTKSGEQVTEQTVMGMDIMDMMVDM
                     PVVSVLMFQKESLTMPAEEMVAGLLRQVHSMK"
     gene            complement(30336..>30854)
                     /locus_tag="PL000_09515"
     CDS             complement(30336..>30854)
                     /locus_tag="PL000_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HNC89181.1"
                     /translation="LFGLLNPSGKLTISIPVHVGQQPIFYSQVRGQHGSRYADLTQEP
                     LFPFGHGLSYTKYTYSNLRLSSSKLAQGDAVKVLVDVENIGQREGVEIVQVYVSDVVT
                     SVTWVNKALKGFARVQLQPGEKKTVEVELPWESFRIVNTKAEYIVEPGEFEILVGPSS
                     RDQDLLKTTLTI"
BASE COUNT         7082 a         7544 c         8458 g         7770 t
ORIGIN      
        1 acaccttcat tccctgtaag aattccacct tgctgttcgt ccaaagataa tctcagctta
       61 aggaatacaa ccttgtccga atactcagaa cgcccctctt tgttgcaaac ctacaccaaa
      121 agcttttctc cgctgaaaaa tcgtaatttg cgaatttacc tcggtggaca ggccatttcc
      181 ctgcttggca cgtggatgca atccactgcg cagagttggg tggtgtggga attgacccga
      241 tcagagagtt cgttgggcat tgtggtgatg ctcaacaccc tgcctctttt cctgctcgcc
      301 ccgtgggctg gcgggatcgc agaccgtttc aaccgccgca tgattctgct tgttacgcag
      361 ggcattgcca tggtgctggc ttttgccctt gccttcctca tccaaaccgg gcttgtgcag
      421 atctggcata tctacacatt tgcattcatc cttggcatcg tcaatgcgct ggatttcccc
      481 gcccagcagg ccttcattgg tgacctttct ggcatgggac aggtgcggca ggccattacg
      541 cttaatatca tggggttgca agtggcgcgt atgttggggc ctgctattgc gggggttttg
      601 ttgaaagtgg ttgggtcagc cgctgcattc tggattaatg gcgtgagttt catggtggtt
      661 ttgctgagca ttttcatggt ccgctctcag caggcgagtc ataaccgccc cagtgcgcgt
      721 ggacaattcc gcgaaaccat cgagttcatc cgctcccagc cgcgcatggt ggatttgatg
      781 gtcttcgcca ccctcgtgac ctttttcggg ctctccgtct tcaacattct cccctcggtt
      841 gccgaccatg tcttgaatgg cgactcgcag acctatggac tgctgtttgc ctcctcaggt
      901 gcgggcgcgt tgatctccac cctggtattg cttccgcttt cgcaggcagc caaacgcatc
      961 ggactcatcg tcagcggtgc ggcggcgtgg atgggtactt ttttcttttt gttgtccaat
     1021 tccacctggc tgccgctttc aatggcggtc atcttttgta tcagccttgg cgcgccgctt
     1081 gtgttgacaa caggattggg tgtgctgcaa ctcatgtccc cgcctgatat gcgcgcacgg
     1141 atcatcagct tgttcaccat gctcaccttt ggccttcagc cgatttcttc catcgtcatc
     1201 ggttatagcg cggaacattt cggcacgcaa aacgccatct tgatcaattc gctttgcctc
     1261 tttgcaggag ccgcactcat gttcttcttc cgtgatgggt tgcgccaatg ggaattgaaa
     1321 cccgctgtcg agccgattct atcctccaag gaaacatccc agcctgcatc tcattagttt
     1381 tgaattaagc aaaaaacatc ccgaaagatt ggtttcggga tgtttttgct taaaaggcat
     1441 ttgcatatct atgtaaaatt aatgtgttgt tttcgttgat cgggctgatc caagccgcga
     1501 ttgcgttttg ctgcgcaggg cggccagtgc ccgctctttt tttaattgtg tttcctcctg
     1561 cctgcgcaag taaagcatga ccagccagta tgcaaggacg aacaaggtga caagggccgt
     1621 gcctatgtaa agtagattgg tgatcggcag tgccgaatct gtgcctgcat ataatccatg
     1681 cagggtggtg ccaaggtagg cagcgaggct gaggtaatgg agtttgcgaa aagtttccaa
     1741 cccgatttgg gttctgaagt aataggtgac cgtgacaagc aaagtgaaat agaaggcgat
     1801 gataccgatc ccaacccaga agggtcgata gggcgaaaga aagggaatta atatctgcca
     1861 aaggctgaag ggaagatagt tatcggccag aagaacgatc acatgcacag ccgtaaaccc
     1921 gaggccgatc aacgaaagat attcatggaa gtcaaaagtg aatgttcgat ggaggatcgg
     1981 gtcgaggatc ttgctgctga cgaccaaccc ccaaaacgtg gaaagccaga tcagaaaata
     2041 tcccatcaac ccggcggcac gggtgagata ccaccaaaat tgcaggctgt tgatcgcaaa
     2101 taagttcgaa ataaattcaa ttggattcat tcaagtgtct ttcaggattt atcaggctta
     2161 cgaggtttcc gcttgcatcc actgcaagaa cggtaagctc ggggttttgc tcttcaaaca
     2221 aacggatgtg ggtttcatct gccaacagga atgccttggc gaaagcttct gctgttactg
     2281 cgtgttttgc aaaaacggtg acgctcaacc agtttgtgtt tgcgggctcg ccgctgcgcg
     2341 ggtcgatcag atggtgacgg gtgtgttctc cctgtttcca ggatcgtttt acaacagaag
     2401 aggttgccac agcgccttcc tgcgcgtaca aaacggagat gttttcagcg gggctgcgcg
     2461 ggtcttcaag cccgatctcc cacacgtcct gtccttctgg ataaccgatc aggaatatgt
     2521 caccgcctgc gctgaccgca caggtggacg tgtattggcg gatcaatctt gcggcttgtt
     2581 cggcgatcca gcctttggca atgccgccta ggtcaatttg catattgggt ggcaatagaa
     2641 tggcttggcg gctttcattg agttgtaaag atgcaaaggc ggaatcaaat gtttggggtt
     2701 gagtggcagg ttgaaggtcg gtgccgaagc gccgaatttc atccatgctt cggttgtatc
     2761 cagcagcacg caagtttggc agaatcgatg ggtcgaataa tccgctggtt ttttgatgat
     2821 actcaagtgc cagcttcacc acctcgaaca tttcatgtga ggcgttgaac catgttcctg
     2881 ccgagcgatt caattgagaa agttcacttg catcggagaa acgtgtaaag cgtttttcat
     2941 ttgtttcgat gaattcgcgg gcggcaataa accccagaac gacttgtgga atatcacctt
     3001 ctgccagaag gatgatctct gaattcattg cgcgaaatcg atcggcttgc atttaggaac
     3061 cgcttgtacg aaggaagaca gtgcgtgggt tgatctgaat caaactttcc gcccgccctg
     3121 aaaacatggc ctcttcaagg gatgggacag gcttgagttt cggcatggcg ggaatgtcag
     3181 atgccttgaa ttgattgggt ttgggcgaat gagccagtac gatccacccg accaggaagc
     3241 tgatcagcga aaccagagtg aaccaaaccc gaatggcagt tttccaaatt ttcattcatg
     3301 ctccagtgtt actgtagaac aacccgacca ttggggtcga tgctgataat gccgcggctt
     3361 tgcagggcca gcaggatcat ttcatattgc tggatctgta ctttggcttg ttcgagttgc
     3421 tgacctgcgt cggccagttc gttttggtag gtcaattcac gggcctgata ctcagccacc
     3481 agtttttgaa gttgggcgat ctgcgcctgt tcagcagaga tgtattctgc agtggcggtt
     3541 gcttcagcgg gggtatctgc aatgggcagg cctttttgat ttaacatggc gctcccgctt
     3601 acgagcgcca tccccgcccc aatgcagagt gtgattagaa atgcagccaa cagggctgtg
     3661 taatgttttt tcatgcttct ccttgcaggt ggaccgattg attgtggttg cgagtatagt
     3721 atttgaaaat gacacagcca tgacaaattt tggggaattt tgtcatcccc tggtcatttt
     3781 gacatgctac aatgaatcca aacggaggat gtgtgcggat tcttttggtt gaagacaata
     3841 aacggttaag cgattctctg cggctcagcc tgatggataa agggtatgcc gtggataccg
     3901 cctacgacgg acaggaaggg gaagacctgg cggaatttac cccctatgat gtcatcattc
     3961 tggatgtgat gctccccaaa aaagacggag tggaagtttg ccgttccctg cgtggcaaac
     4021 gagtgcaatc gccgattctc atgttgaccg cccgcgatgc actggaagac cgtgtgcttg
     4081 ggctcgacag cggcgccgac gattaccttg tcaaaccttt tgagatggat gagttgctgg
     4141 ctcgtctgcg ggctttactg cgccgcgaat cagaagataa aagcgggtta ctcgctattg
     4201 ctgacctggt gttggaccct gccacacatt ttgtcaagcg gagcgggcaa tccattgacc
     4261 tgacttcgcg tgaatatgaa ctccttgaat acctcatgcg taatcccaac cgcttgatca
     4321 cgcgccaaat ggctgagaat cacctgtgga gttacgatgc cacagttcaa tctaacgtgg
     4381 tggatgtgta tatccgccgt ttgcgccgca aggtggatga ccccttcgaa acgaaattga
     4441 tcgaaacggt gcgcggttcg ggctaccgcc tgcgccgacc ctgatggatt gtccttgaaa
     4501 aatatggtta actccctttc ccttcttttt cacagcatcc gtttcaggct ggcgttgtgg
     4561 ttcgttttaa ttctggcgtt gttgctggcc gtgttcagtg gattgttgtt ctacatgcaa
     4621 attcaagaat tgcgcgccga gaccctgaat cgtttgcagg gaaaactgga gcggatcgaa
     4681 tcggtctatg ttgagggcgg ggtggatccc attgttttag gcagccttaa tggaggggag
     4741 gcgccgcttg accgctcaga tatccttgtt tatttttcac ccaatggaag tgttttgaaa
     4801 atgtgggggc cactttcaaa ggcgccagtg ttgaaccttg ccagtgtgga agtggaggaa
     4861 cacgaaccag ccgatgaaac catcaccctt gaaggcgatg acgggcgcgt tgaatatgta
     4921 ttcaagatca ttccgctttt tgtcgggcag gatcacactg gttttatggc tttgggcaca
     4981 ccgcttgacc cacgtgaccg tttgggtaaa ttgcttttca ccctgatcgt gttgaatggg
     5041 tttacgttgg tatttgcgtt ggctggcggt ttttggctgg cagaccgcgc catgcgccct
     5101 gtcaagacga ttgccgcgaa ggcccgttcc atcagcgaaa cgaatctcgg tcagcgtttc
     5161 aatttgccgc aaaaggatga gatcggtcaa ttggctggga cgtttgattc catgctggcg
     5221 cggcttgaat cggcttttgc gcgccagcgt cagtttacag cagatgccag ccacgagctg
     5281 cggactccgc ttacgatcgt aaatttggaa accacccgtg cattgtctgc tcaacggtcg
     5341 gtaaaggaat atcaacgcgt attgcaggtg attcattcgg aaaatgaatt aatgagtcac
     5401 cttgtgaatg acctccttac ccttgcccgt atggatgcag gccgcgaagc cctgcacagg
     5461 gaagttcttg acctcagcga tattgccctt gaagtcgttg aacgccttga acctttggcg
     5521 gctcgtcaac aggttcatct ttttacaggc gagttgccag aagtgaccgt attaggtgac
     5581 cgcagttcac tcacgcagat gcttacgaat ttggttgaga atgcgatcaa atacacggcg
     5641 caaaacggac ccgatcttga acgacatgtc catgtggagg caggtacgca tcctgcgcaa
     5701 gcccagggct gggtgcgagt ttcagacaca ggtccaggca tctcaagcga acacctgacc
     5761 cacttgtttg accgtttcta tcgcgttgat aaatcacgca cacgtgaaga ggatgatgaa
     5821 gttcgcccaa cagggagtgg gttggggctg gcgatcgtgg acggcatcac ccgtttgcac
     5881 aatggcagca tcgaagtgca aagcgaattg ggaaaaggtt caacttttga agtcgttttt
     5941 cccctgcatt tgacataaag gaccttcaag caaaaatcac ccaggagtta attttggttg
     6001 atttcattga gcttgatcaa caccgcctcc agcacacccc cgccgactga aagcgctttg
     6061 tctgaaacca gccaacatgc actggtatca ctgcgcggat caacatcgcc gatcttcatg
     6121 ccttcaagca cttcaatatc agggcggatg atcccacgca aaacgccgtc gaatgggctg
     6181 gtgatctttt cctcgccgat atctgccaca agctgccctg cctgaagcaa atcaccgatc
     6241 tctgcgtatg cggtcaaaat ccctgtttgg ggggcgcgta acacacggcg cggatcaccg
     6301 tcaggctggc cgctatcggg ttgagtggct ccgctccagt aaaccctgcc gagggtgtga
     6361 ctgcggcggg tttctattac agcgtggcag ttctcacctg cgctgaatcc aggcccaagt
     6421 ccaatatgaa gcggaacgtt cacttccaat ggatcgggcg cagttttcaa gagtcgcgca
     6481 tccactacaa aggtgaattg cggacttatc aaaaattgat tgcgcaggat gtttgcgtgc
     6541 ggatcgacca acacgggaat ttcacccgcc tccaacgtaa cctgaaactg gtccgccgaa
     6601 acgagacgcg ccgtcacccc ttcgacagtg tgagtgcctt cgtacaccgc ctcggaaaat
     6661 gagaccgcgc ggcgcacagc cagcggtttt tccaattcaa gaatgacgac ttgaaaacct
     6721 gtgcgatgca ggcgcaatgc gacaccgctt gcaaggtcgc cgccgccgcg aaggacaata
     6781 agatgggtca tagagtttcc tgtgaagttt taggggcctg ttcctgaagc aaacggccaa
     6841 aagcgtaaat ggtggtgtaa aagaaaatga agaaggttgc gaacacgctg ataaaaatga
     6901 tggactgcat gctggggtga gcggcgtaat aaagtaattc ggtcagccgc ccaagcgggt
     6961 tgaagaacat atcccatgaa ttccagcgca ggaacctgcc gatgtaaata ccaaggctgc
     7021 tgagaaaccc aaccgcaaac acaaagaacc agcctgcaat gccgccaaac tcacggcgga
     7081 caatatcctg catgagaaac agcgaaacca tgccaagcag gagacctgtc catgcaaacc
     7141 agatgatcaa cagcacatca aaccaaacag gcacacctgg tttgggagag ccgaggtgct
     7201 gcaaatcggt cagaatgtag ggagcgtttg ggaaaaacaa caaccagaga gtcgccgcca
     7261 caggcacgct taagacgatg tatctgcgct tccatgcaaa gtgagtggcc acgtgtgcga
     7321 acccaagcgg aatccatgcg aggaagatgt tccagagcag aaacacataa tccagtgatc
     7381 cgcttaaaag ggtgcgcatg cgaaagagca ggatggaaag cagggtggct ccacacaaca
     7441 gcgcaatgac cgtcaagcga aacttgatgg gatggatgtg ctcgtaaatt ttcttcatgg
     7501 ttttgacctc cagttttgag gccgattata tgaataccga cttccaaacg ctttcaatga
     7561 ctcgcaagtg cggcaacagg atcttcacgg acaagatgtg cgaaggaata cagggtgaga
     7621 tagacgaaca gataaaagac cgcgaatagg atcgtgaacg cgatcacccg catggatggg
     7681 tcaatggcaa ggccgaggat ggtgacgacg atttcctttg ggtcgttgag caggtcccag
     7741 gaattgaaac ggacgaaacg acccagatac acaccgaaac tgctcaaccc tgaaacaatg
     7801 aatacgaagg cccagcctgc ggcgcgccca aacatgcgtt ggataatgta gtgcatcagg
     7861 tacagtgaaa ccaggccgag caacagtcct gtccacgaaa accagaccat cacaatcaca
     7921 tcataccaaa gcggcgcgct ggtggattct tttgccaggt gctgtaaatc ggtgagaatg
     7981 tatggcgcat tggggaagaa gatcagccac aaaaacgtgg tgacagggat aacgaaatac
     8041 aacagccact ttttccacga aagcgtgtag gcgagatacg ccaacacgaa cggaatccac
     8101 gcgaggaaga gattccacac caggttggtg tagcggatcg aatcgctgta ggcaatgcgc
     8161 gccgccacaa ggaagacaca aattgccgag gcgacagtca tcaagccgaa gatggcgatc
     8221 ttgtaacggt tgcgaagcaa aaagtttttc atggctttta tttcaactca cgcaaaaaca
     8281 cgatcaagtc cacatcaccg ggggagaatc ccagctcggt gagtatggca gcagcttcac
     8341 tgcggtgctg ggtgccgtgg ttgaccacat gtgtcatcgc ctgccagagg atgttttcca
     8401 tttccacacc gcgggtggtt ttgaattgga agggcgcatt cagcttttca tctgtcaatg
     8461 cttcaagaaa agcactcaag agtcggtctt cttcctgcca gcgtgtacgg agtgcttcaa
     8521 aggtggggaa atcttcgggc ttgagaaaat aggtcggtga attgccttcc caacgctggc
     8581 gccagatcca ttcggccatg agggtgtggg tcaatgtgcc gtgcagcccg ccatgagggt
     8641 acgaatcggc ggcgagatat tgctcagcgc tgaccctggc aacagcatcc aaaatacggg
     8701 cgttggccca ggtgttgtat ctgtaaagaa gttgaatatc ttgtttgttc atttttctaa
     8761 aaactccatc aatatcttgt tgacgatctc tggtttttcg tagtgcggaa tatgtccaca
     8821 ggcttcgatg gcatgaaatt ctgcatgggg aatggccttg agaatttcca tgctgttttc
     8881 aaagggcacg gtcgtatcat ttttacccca gatcagcagg gtattttttt gcagtttccc
     8941 cacgcgctcg taagtggaga aaaacgattc aagcatttga tttcgcatcg tcgaaaggat
     9001 cgcctgcttg aaacctttgt actgcatctg gatcttatac cgcgcctgaa aatgctcgac
     9061 cagatctgga tcgaacaggt ctgaggcaat gcccttgacc aaactcacgc ttccaaacag
     9121 accgagggcc agttcgccaa aaatgggcag tttcaccgct tcgagcagaa gtgaaagccg
     9181 cacccgtttt gcgccagcag ggtcgatcag gatgtgtttt tcaacgctgt gcggattttc
     9241 gtccatgaag gcggcagtga tggggccgcc cattgaaaga ccgagcaggt gaacccgcgg
     9301 caggtttaac gcagcaagca gttctttgag ctgacgtacg aacaggtgaa tgttgtatgc
     9361 ggtttgcggc ctgtcggatg ttccgcgccc gaacaaatca taacgcaaga cgcggaaacc
     9421 tgcttttgtc aaaaagtcga atgtggggtc gaagatgaag tatgggacgg agaatccgtg
     9481 gacgagcacg atggcaggcg ctgtttcggg gccgaccaat tcaaaatggg tcgtgccatc
     9541 tgtcagctta atgaaggaac cgcccgcttc ctggcgggct gattcgttca agtctctgtt
     9601 ttcgtcgaga taggggaagg gcatggtctt tgcagggata cagcgggaac tgtatccctg
     9661 cgaggtggtt gtttatattt ttccaagtgc gcgcaggacg cgttccgggg tgaaggggaa
     9721 ttcgctgatc caaacgccgg tggcatcgtg aatggcggcg gcaatggcgg gagcaacagg
     9781 caggaagggc agttcgccca ggccgcgcgc tccccatggg ccgttggggt ctggcacttc
     9841 cacgagaacg gaatccactt tttcgggaat gtctaaaatg gtggggatga gataggtgct
     9901 gaattggtcg gtgaggacgc ggccgtcttt ggttttgtaa tcttccatca cggcgtaccc
     9961 atgagcctgt accaccgcac cttcgatctg cccgacgaca aggtcagggt tgatggcttt
    10021 gccgacatcg tcggctgagg tcaggcgtaa tacgcgcaca tggcctgttt cagtgtccac
    10081 ttccacttcg gcggcttggg caacataggc gtaggagaaa ttcggggtgg agtagcctgt
    10141 ttctttgttg aatggcgtgg tgcgcggcgc gaggtatttg aactctgcaa cagcagggcg
    10201 ttcttcgttc ttccattttt cgagggcggc ttcagcggcg cctttgaccg aattgcctgc
    10261 catgaaggtc aggcgtgatg cagaggcgga accggggttg ccttgtttgg ctgaatcaga
    10321 ggtgcggatt tcgaccttgt ccatgggcag gccgagtaca tgcgctgcca tttgaaccat
    10381 gaccgtgtgc attccctgac cgacatcggc acccgcatgg tggacgatgg cgcgttccat
    10441 ttttccattg ccgtgaatct caaccttggc ccagcagttt tcctgatagc caaaagagaa
    10501 gccgacattt ttgaagccag cagcgaatcc tctaccacgt acgagcgggg acgatttttt
    10561 gccagcggcc tttttcgctt tcgatttttt ccagccaaac tgatcgcgtg cggcttcaat
    10621 gcattgtacg atgctgaccg ggcccggggc gggtgtgcca acgcccatgg catcgccatc
    10681 gcgcagggcg tttttcaggc ggaattccac agggtcaatg cccagatgtg cagcaagttt
    10741 gttcatttgc gattcggcca tgaacaaagc ctgtggggcg ccgaatccgc ggaaggccgc
    10801 gccggggaca ttgtttgtgt acacgccgta cacatccact tttacgttgg ggatgaagta
    10861 tggccccgtg gaggtgatgg cggcattgcc aagcacttta ttggaggtgt acatgtaggc
    10921 gccgccatcg ccgatcattt ccatttctgc ggcgatgagt tttccatctt tggttgcacc
    10981 ccatttggct ttgacagtgg cggcgtgccg cttgccgtgg ccgatcatgg attcgcggcg
    11041 tgaccagatg atctttaccg ggcgctgtaa tttccatgtg gcaagggcca gcacgatctg
    11101 cactgaaagg tcttcgcgcc cgccgaatgc cccgccaatg gctgggtaga tgatgcggat
    11161 catttcattg ggcaggccga gtgaatgggc gatggtttcg cggtcggcat gagtccactg
    11221 gccgcctgct tccacggtga tgagtccgtc gtcgtcaatg taagagaggc ccgcttcagg
    11281 ctgcaggtag gcgtgttcct gtacgggggt gtggtattca ccttccacga tgacatctgc
    11341 atttgcgaag gccgcgtcca tgtcaccttt gcggatcttg tagtggacgc agatgttggt
    11401 gtcgcctcta tcggggtgga ggatgggtgc atcgggacga agggcagctt tcgggtcgga
    11461 aagaatcgga aggtcttcat attcgacctg aatcaacttg atcgccgcat ccgcctcgac
    11521 ctcggtcctg gctaccacga ccgcgatctg gtcacctaca aaacggacaa tgtcggtgtg
    11581 tggttttttg gatggtccgc acagaacagg ctggtcgggg atttgcaatc catattcgtt
    11641 gaccggtaca tctttcgatg tgtatacggc tacgacaccc ggtgcggcat ctgccttgcc
    11701 ggtgttgatg ttttttacgc gcgcatgtgg acgctctgcg aagagtatct tcatgtgcag
    11761 cataccgtct tttacaaggt cgcctgagta gagggctttg ccggttacct tgtcatgggc
    11821 atcgattctt ggcaatgatt tgccgactga ttgattggtc atggtttcct agcgcttgta
    11881 cttttttact ttgattccgg agggaggggc atctgtcggt ccatagcggg atgggccaat
    11941 ggcgcgatag atcgcggcgt agatgaccga gatgacgccg ccaatgagca tcatcagaac
    12001 caggctggct gctgcaatgg caagcaggtt tggaacttcg ctaatggggc taaagatcgt
    12061 tgccagcccg ggggttgagt agaaaatctc gggcaggtac actttgccgc gcaattcgta
    12121 gggaatgggc catttgtttg cgataccata ttggatcaac tcataggctg atgcaattga
    12181 tattgcagga atgatcaaga tcatcaaaca gccaaagccg cgccagatct cgtgcgttcc
    12241 tttggattgg atgcttgatg gcttcaaggt tgtgttgtta ttgtatttgc ccatcagaat
    12301 ttctcctacc tattaaagtt tgcttgcatc ttccattgct ttgacgatct tgtaatagcc
    12361 tgtgcagcgg cagagatttc cggtgatggc ttgttcgatc tcgttgcggg ttgggttggg
    12421 cttttcttcc agcaatttgg ccccagacat aaggaagccg ggggtgcaat atccacattg
    12481 cacggcacca ttttcaatga atgtttcctg aatgggatgc agggtacctt cttctttggc
    12541 aagcccttcc acggtgatga tctctgcgcc atgtgcgcgt ggtgcaggga cgagacaggc
    12601 catgacggcc tttccatcga ggaaaacggt gcaggcgcca cattcgcctt cggcgcaacc
    12661 ttcttttgtg ccggtcaaca tgccgtcttc gcgcaacaga cggaggaggg ttttatcatg
    12721 accttgtttg aaggaatatt tcttcccatt gatggtggtt tcgatggggg cggttggaaa
    12781 ttcactggtt gcctgcaggg ggtgttttgc aacgtcagtg cttgccagca aaatcggctt
    12841 ctttggcatg ccagcctgtt cattttcgtc gcggatggcg cgaagtccgc gggcggtgat
    12901 gatcttgacc atctcgcggc ggtaggcagc ggagccgcgc acatcgtcaa tggggtgtgc
    12961 ggcttgtgtg gcaagttcgg cggcgtgggt gatgttcttt tcggtgagtt tctttcccgc
    13021 gaggtatttt tcggcatctt cagcgtgggt gatgatggga gtcactgccc caagggtgat
    13081 gactgccgat ttgacagtat ccactttcag gtcaatgatg atcgcgacgt tcacaagcga
    13141 aatcgcctgt gcgcggcgca gggcaagttt gatgaaggtc ccgcgctgat tctttttcat
    13201 cgctgggaag gaaatatcca ccagcatttc atcaggctgc atgacgttct tgcggacgcc
    13261 tgtgtagaac tctttgagtg gaagggtgcg gctgccgcgt gtggagagca gggtaacgct
    13321 tgcgccgagt gccatcaacg gggtgatggt gtcgttggcg ggtgaggcgg tgatgaggtt
    13381 gcctgccacg gtgccgcgat tgcggatctg cggtgcgcca acctcccagg cggcgcgggc
    13441 cagcgggtag gcatgtttgc ggatcacctt ggaagctaca caatcattat gtgtaacccg
    13501 tggcccaagg tggatcacgt tatcttcgtc tattgtgatt tggtcaatac cagggattcg
    13561 tgacacatcg atcagtgttt caattccctt gcggacaccg cgttcgagtt cgaggatgag
    13621 gtcggtacct ccagctacga cccgggcgcg ttcctttttt tcccctagaa ccttgagtac
    13681 atcttcaatg gatgttgcgt tgatatattc gtgccacata gttaatgtta tagggacacg
    13741 gcgccgccgt gtccctacca taaattaatt cccgcttccg gttacaactt cggagcggcg
    13801 ttcaaacgtt tccacggcca gtcttgaaag tgtggtcagg ttgcggatgg cagcgggcag
    13861 ggcgctttca tcgttcagac tcgcttccac attatccagt gcgctgctga cttgttctcc
    13921 aagtgtaaag aaggcaacgt cgaactggta gatggcttca agttcctttt cgttgatctt
    13981 aatcgcatcg aacatgcctg aataaccgcg tgcagccctt gaaattttgt caataaaggt
    14041 gcgcatggaa agtgtggctt tttccatatc gtccacgtag ctgagcatgc cgttgctgac
    14101 aagttcggcc tgcatgttcg aagcacgact ccagtattcc tcgaagcggc gggccaccac
    14161 atcacgcagt tctttgtctg cgtcgcggcg ggtttgtctt tcaacatatc ccttgaaccc
    14221 tgggatgtac gaaagaagtt tcttgaacgg gtcgatctga ctacttactt tttcaaaaaa
    14281 atcactcatg tgagcctcct gccatagtat acgtaggtct tgccaaatcg tctcacgatt
    14341 ttttatatta tatcgtttat ttccagttat aatcaagtta tcattttttc aggagttaac
    14401 tatgtctgag catgtcaaag aattgttgga attagttgaa aatagcctgg cagtggatgg
    14461 ggcggctgtc aaggtggttg atgcggcgaa attcaggaag aatgtgggga agctggtgga
    14521 acgctctgca ctcggttcgg attcaactgt gggctggtcc cgttaccttg tccgtgcagc
    14581 cgccttggaa ttgggcgtgt accctgcttc catccatgag ttgtaccttg ctcgcggacg
    14641 cggcgaagca ccgatgacat ttaccacccc cgcattcaat ttacgggctt tgtcgtatga
    14701 tgcggcgcgg gcgatgttcc gtgcggcaaa caagatcaat gcgggcgcgt tcatttttga
    14761 gttggcgcgt tctgaaatga gctataccgc tcagcggcca tccgagtatg ccaccaacat
    14821 cctcgcggcg gcagttgctg aaggatttgt cgggcctgtg ttcattcaag gcgatcactt
    14881 tcaggtttcg gcgaagaaat attcggccga tgccgagggt gagttgaaag cggtccgcga
    14941 tctttccatt gaagcgatga acgcgggttt cttcaatatc gatgtggata cttccacgtt
    15001 ggtggatatt catcttccga ccgtgcctga gcaacagaaa ttgaattgtg aactttctgc
    15061 tcagctttcc gcgtttatcc gcgagaatga gcccaaaggc gtaaccatct ccatcggtgg
    15121 tgagatcggc gaagtaggca cgaacaactc caccgaaccc gaactccgcg cttacatgga
    15181 cggctacaac cttgaaatga agaaacttgc ccccggaaag cctggcttga gcaagatcag
    15241 cgtgttgact ggcacttcac atggcggcac ggtccttgcc gatggttcca ttgctgaagt
    15301 caccattgct tttgatgtgt tgcgtgatct cagccgtgtt gcccgcaagg attatggcat
    15361 gggcggcaca gttcaacatg gcgcttcgac cgtggcagaa gagaatttca acaaattcgt
    15421 tcagaacgaa gccatcgaag tgcatcttgc cacgaatttt gccacgatgt tcttcgacaa
    15481 tattcccgcc gacctcaaga aggaaatgta tgcctggttg gatgtcaact ctgcaggtga
    15541 ccgcaaacct ggcatgaccg acgaacagtt ctattacaag acccgcaaaa atgcgatcgg
    15601 accgttcaag gcgaagtcgt atgccctttc tgaagcggcc aaagccaaac tcggcgcagc
    15661 gtgggaagcg cagttcgata agcttttcaa cctgctgggc atgaaagata cgaaacagta
    15721 tgtggagaag tacattccca acgtgaaggt tgccccggtg ctggcacatt atgtaaagga
    15781 agatgtcgct gctgaagatg taagcgacct ctcggattaa tcccccattc tgccttgaac
    15841 aaagagaaat gccaaaatca gaccaaggcg taaccaaaga tcgatatatg ctcattcccc
    15901 gcacggcgat ttttctccgt cgcggggatg agtatttatt aattaaaggg gctcccacaa
    15961 aacgattgtg ggcagggaag tacaacggtc ttggcggtca cgtagagcaa ggcgaagacg
    16021 tgttcacctc tgcgcggcgt gaattgctcg aagaggcggg gctgactgct gatttatggc
    16081 tgtgcggcac ggtgatcgtg gatgccgggg aaacaggaat ctgtctgttt gtgttttgtg
    16141 gagagaatgc ccaaggggag atcaaggcca gtgcagaagg agcagttgaa tgggtccgct
    16201 ttgacgcggt ttctcatctt cccgtggtgg aagacctgcc catcctgctt gggaagatac
    16261 acagtatgaa gcatggtgat gcgccctttt cagcgcggtc acattatgat gaaaatgaaa
    16321 gactacaggt tgtatttggt gtttagaacg cacggggaaa gctggtgaat ttgaagagtt
    16381 cccgttacgg aatgttatag ctttgtagtt ctgtggtggc cgcgaaggtg gttccaccaa
    16441 aatcaccgtt ttcaacagat ttgacgaagc ccacgcccgg cacataccaa ctgatggttg
    16501 tgccgttgaa tttaatgggg acatcaatcc cttcaaaact tgtgacgata tcaatggttg
    16561 agttggattg caatttaacc gcttcaaacg tgccagctgg gaccgtgatg gtttcccgcc
    16621 cgatctcctg tgtggtcaca gaatataccc cgctggattg cgcctgctgg tcgccaggca
    16681 tcgccatggt tccctgcatt ttcaggctat aggcccattg catgcctgga gttacttcct
    16741 tggggagact gatgccgttg atttcaatgg ttgtgaaggc cgcggtcatt ccaagtgtgg
    16801 aaattcctgc ggctgtgccg ccgccaagtt gtaaagcctt caggccgtcg ctctgacagg
    16861 cccattcctg tgtgcgtatc aggtctgcga attgtgatgc gatcgtgaac ttgccgtcaa
    16921 gtacggatgt gaccgtatct gtgtatgtga atgaaccgcc tgggccgccg ttgctgacat
    16981 aaacccacat ggcgccttgt ttgacaggga gaagggggtt gtcacatgtt ccactgatgc
    17041 tgtcagagga accagattgc tgagattgtg attctgttcc gccactgcat gcagttaaga
    17101 tcaaaacgat cgagatgaaa aagatgaata cgcccttgct ggtatggtac atgcttgctc
    17161 cgatgtttat ctcaggcagc gattccaagg atgtaggctg cgggaatgtg cctgtcgtgc
    17221 ccgttctttt attgccactt caacagggag atcgtaaatt tgcttccttt gcctggctgg
    17281 ctttccacat ccaacatgcc attgtgttga agaattacct gacgggtgat ggaaagtccg
    17341 atgccgaggc cgctgtataa ctcgtcgccg cttttttcca aatggaagaa ccgatcaaaa
    17401 atgcgcggac gaatttcggg ggcaatacct atgccgcggt cttccacgga aatgagaacg
    17461 cgttcccttt gttctgaaaa acgcacttcc acctcactct tcggtgcgct gaatttgatg
    17521 gcattatcca gcagggcagt gacagcccgt gaaatggatt gcgcatcgcc gcgtacgttt
    17581 gaaattccac gctcaccctt gaccttgatg gtaacaccac gggactgggc tttactttca
    17641 tagcgtttga tcacatccat ggccacttca acaagattca ccgattggaa ctcaggcaga
    17701 accaattcga tctcttgaag aaatagcagg tcgtttgcca gtgtggtgat ctggtctacg
    17761 ttgcgggcga tcgtgtccac ggcaacatta aactgatcgc ttgtgatcat tccctttttc
    17821 atggcctgca ggtaaccgct ggctacctgt aaaggtgtgc gcaattcatg ggcaatgttg
    17881 gaaaggaatt cgcgccgcat taattcctgc tcggcgagtt tttggtaggc atctgccaat
    17941 tccgcagcgc gcagacgtaa atcatcaatg gcaagctggt cgttttggcg caggcggttg
    18001 ctgatctcac tcaccatggc catggcaacg ctggggctgt gttttagaac acgatcaaag
    18061 ccttccttat taagctcaag cacgatcaca tttgtcttgg tctttacggt ggcggcacgc
    18121 ggcgcattat ggataagcgc catttcgccg aaaaaatcac ctgcggtcaa cgctttcaga
    18181 atgcgtattt ccgtattgtt gatcaccttg gatacttcaa aatctccctc aagtaccatg
    18241 taaaaagtat tttcaacctc gttctcccga caaataatgg tttcaggcgg gtaagattga
    18301 atgcgactat taataatgat ttcctgcact tcttcaggct tgataccagg gaacgcgcgc
    18361 ggaatgattt tggctggtgt gcgtgtattg gtcatggatt tactctatgg attctcgaat
    18421 gcaatttcct gattttatca tgcacagaaa ttgcatcctt cacccattcg gactacaagt
    18481 aaattttagc aaaaaatatt cgcaaacaca tgtctgaaat attacatttt cgaaagggat
    18541 tttgctctat aatccaccaa atgtatgcca gaacgaaatt tcctgcccgc cttctttgga
    18601 tgacgcttct cgcatccatc ttcgccggag cctgccagcc cgcattgaca gagtcagcaa
    18661 cggtgacgcc tctccctact tttaccccca ccccgtatta caccgaaaca cctacaccca
    18721 catccacacc ccagccaacc cccacgccac cgcgtctggt cccgaatttc acccatattg
    18781 tcattattat ttttgaaaac aaggaattcg gtaccgtcat tggcaacaac cagatgattt
    18841 acttcaatct gcttgccagt tcttacaccc tgttgaccga gcattatgct gtgactcacc
    18901 ccagcctgcc gaattacctt tccctggtca gcggcgatac cttcggcatt accgacaatt
    18961 gcgaagattg ttacatcaat tttctcagcc tgcctgatct tattgaacaa agtggacgaa
    19021 cctggaaaac ctatcaagac gacatgcccg agccgtgttt ctcaggcagc accctgcgtt
    19081 atgtgcaaaa gcacaaccca ttcatgtatt ttgattcgat ccgcctgaac ccggagcgct
    19141 gtaatcgcag tgtcgtcccg ttgacgcagc tggatgaaga tattgcggca aatgcactgc
    19201 ccaacttcat cttcatcacc cctgatattt gttactccgc ccacgattgt gaattggaac
    19261 ttgctgatgg ctggctgaag gaaaacgttc aaaaactgta tctggcactt gaagccacaa
    19321 aggagcccta ccttatcatc cttacctggg atgaagggca gggcactcat tcctgttgtg
    19381 ggcttcctga gcttgcaggt ggaagggtgg ccacagttct aatatctccg caggtgcggc
    19441 aaagttttca agatcacacc ccatacacac actattccat tttgaagacc atctccgaag
    19501 cctgggcatt gccttttctc ggccatgcag cagatgccaa tactgccatc atagcctctc
    19561 cgtggaagtg atttctttat tcgccaatac ggtgatcgta aaagaccaaa agcgtactgc
    19621 cgtattttct ttgctcggtc tcttcgaggt tttccagttc cacagcctga tactcggttg
    19681 ggtcaatttg tacaatgaca gtcccgtcgt ctgtcagcca atccatgtgg gcatccacca
    19741 gctttagggc ctccagccac atctctttat actgtggtgg agcaatgtag atgtattcaa
    19801 aacttttatc ggccctgcct gaaagcatgc tgaaggcgtc accccgccgt atctcgcttt
    19861 gttgtaaaaa cccgcagtgt gttacgttct ctcgtatggt ttcgatggga gcgcggttga
    19921 gatccgagaa acgtacaaag gccgcgccgc gactcaaggc ttcgatgccg attgcgcctg
    19981 tgccaccaaa tagatcccac caattagaat cgatcacgtc atcggcaagg atattgaaca
    20041 gtgcctcttt cacacgatcc atcaccggac gtgtggtgtc gccaggcacc gatcttagtt
    20101 ttctgccccg cgcggttcca gcaatgactc tcagactcat tcaacgctct ttcttgcaac
    20161 cagcaaattt ccgtgtgatg cggtgatggg aaccacgcac cctgtgccaa ggatgaaacg
    20221 atgactcttc gtctgctgga tggcatccgc cgcttcttcc ttgattttgg cttgatcctc
    20281 cagaacaaga gtatcttgcc tcaagccacc gcacaatact cctttgaaga ggctatgggc
    20341 ttcggccagg gatggatgtg tttcgcggtc gtgccagttg acgatttgga agttgagcag
    20401 acgcagcaag gaaaagtaaa cgtctttgcc gtgcaggtga agcatattgc accaaaggtt
    20461 tttgacagga aacagtgcct gctggtcgta aggcaggcca aaggttgtgt actcatccac
    20521 agaaagcagg ttggcttggg cgtgctggac agcataaaaa ataccgtcaa tgccgatctc
    20581 agccgcggct tcgatgaact ttgccgtggt cctggcgatg gtttcaagtc ccttcatgac
    20641 ggcttcaggg tgctgcctga tgtgtgccac aagggtgtca ttgctggcga ggtttttcgc
    20701 ctgcgaaagc ggattaaaga tggtttggat gagcggagtg tctgggccga gattctctcg
    20761 aattaaacgg aggcaggtca gttgtcccgc gagatgcggt gaagttgggt ccagcacggt
    20821 gagcttttcc cagtcttcag gtttgtggat gacacgtttt gtgtatcgtc ttgaaccctc
    20881 ggagtcgccc atccatttat cttccacccc ccagtctttg actgcaaagg aggaagcggg
    20941 tgtgactttt acgatgtcaa aatcataggt ttgttgaaaa tgcaacgtgg aagccgcaag
    21001 ggtctcgggg ttctggtcat ccacggggaa gtgccgccag agggcaatgg gtgtgcggtc
    21061 tgtttttttg ccttcaatac tggcctggat acgttctcga tgtgtggtca tgggattttt
    21121 tttatctcgc agaataccgc gtcaccgcgg cggatttgga ggacgacagg cgcctctttg
    21181 aaagttttga tatcttcatt tgcgcgtgtg ttgacgagga caaaatcgcc tgatcggatc
    21241 tcgccaaact cggcgcggta atcacgaatg ttgaggtttt cactggcata atacgaagcc
    21301 atgatcggct cgcgcttgac aaagagttgt gcgttctgtt ggtcaaattg ttcaactgca
    21361 tctttgtagc aggtgagcca gtaatctgtt tcaaaggtgc ggaatgcgcc gcttgtgccg
    21421 ccagtgaaca gattgtaata cgcatattga taggggtgaa gttgtacccc gctgtatatt
    21481 gcaggcgcaa tgaggagggc ggcaactgca acggcaattt ggcgggattt gatgatctca
    21541 aataatttct cgaaggcgaa ccctgtgaaa ataaaaactg gcggtagcat aaacaaaaaa
    21601 tggcggtagc catcatacat tggcggcttg cggaggatga cgtataaaaa tggaatgaga
    21661 aaccaaagcg ttggcaagat aaaagaagaa agaacggtta ttttacgagt tgtgatattg
    21721 acctgatgcc aaagtgccgt caacagaccc gcggcaaaaa gaaaccatgt gggttcggtg
    21781 agggtgagaa cgagcaaggt gggcaggtat cgacggggta attcgtcggc acggtacagt
    21841 tgaccgttaa acagcacctg caattgagta gggttgtcgg acatgaaccc gaaggcttcg
    21901 ataaattttt gaattggatt tccccacaaa tatggccagg ttataaaggc ggtcaaaatg
    21961 gcgagggcag aatacagtgc gagttgcttc accaatacag gtattccttt ttgcccgaat
    22021 tgcatgaagg cgtaaataca gaccagcagc ccagccagcg gccccaacac acggatggat
    22081 gtggcaatgc caaggaaaaa ggccgcaaga agtgttgacc tccacaatgt gaattttgtg
    22141 gtttggctgg tgtgaagtgc atccaccatc tccaggccaa aacaaacggc cccgagaaaa
    22201 aaaacgagga acggcgggtc tttcgggttg ataaaagcgt gtccccaaag aagaggctgc
    22261 catgcaaata aagcggtggc agccaatgca gcagattctt ttacccagcg ttttgagaga
    22321 cggtaaaaaa gataaacgcc aaattgaaat gtaaggaaat tgaccagatg ccatgcagaa
    22381 gcattgtcca atttgaatga ttcaagcaca gatacaacag gttgcgccag cagaatgtag
    22441 gcgggcccgc ggttggcatg atctgacccg ctggagccaa aggcattttc gaggtcgaaa
    22501 tttccgctga accattcttt tggagaatac gcatatccaa gtgaatgggc gtaatcataa
    22561 aagagcggct catcccagga taagccgtaa ttacgaaatg tgagcagccc gatcacaatg
    22621 tttatgatga gcagaatcag aatggggtac tggcggactt ttggcacgtt ggttttattt
    22681 ttgcgaaatg cggtgggcct gtcgcttttg ggcctgttcc atgcgttgct tttcttcttc
    22741 ggattcaatt gccaaaggcg gaatggttgt gggatgtccg ccatcatcca atgcgacata
    22801 aacgagatag gcggtgttgg tatgtgtgcg ttcgcctgag acggggtttt ctgcgatcac
    22861 ctgtacttcc gcttccatgg aggtgcggcc cgtataggtg acttctgcat tgagaatgat
    22921 aaggtctccg attttgatcg gttcacggaa aaccatcgaa tccacggcta cggtcacgac
    22981 tttcttttgg gcatggcgca tacaagccag tgcgcctgct tcatccacca gtttcatgat
    23041 ccagccgcca tgcacattgc cgagcaggtt ggcgtgttcg gggtgcatca attgcgagag
    23101 ggtcacccgg gagtggcggg cgctttttgg ggtgagagtc tctgtcatgg gcagaatcag
    23161 tccaggtcct ggcgttgttc gcctgcatcc agcgtatgca gacgttcagg ggcgtcaagc
    23221 aaaatgtcca ctgcggatgg tcccaggtcg ggcatcatcc gcgcgagggg gacggtggcg
    23281 ggcggaaaaa ccttttcagg ctttgggggt ggctgttggc ggggtttaag tgtggacgtg
    23341 gcccgtttgc cgatgatgcg cgggtcgttg gtcagtgtgc cgacgaagta cccaagcaca
    23401 gaatatacct cgcgcttctg gttgacgaag ccgatccaat cgccaagacg attgaacagg
    23461 tacgggtaga gcaggaacgc ttctgcatcg cctttgctgg tgtaaatggg gataaggacg
    23521 ggtttttgtg ttgacatggt caagcctcac tggagtggtc aatatcatta tacgtccagt
    23581 aacggtttac aaaaaagttc cagatcatca caatgcccac agcagaagcc agggtgatgt
    23641 tttttgcata gaactctgct gtggcatatg aagcatgaaa aactccttca aaaaaatgga
    23701 ggagtggcgg ttcaagataa tgcagggttg ggatgcgaat caccacccct gcaacattta
    23761 ccagaaagaa catgccaagc tggttgataa gcggccgtga acgtgattct ggataggtcc
    23821 aaaagcggtt ccagatgaaa ttgctgacga ctgcacagat gaaggaaatc gtcccggcgt
    23881 atacgaggga catgtcggtg aagtgggaga gcaaattcat cacaccgaaa tcgatcacag
    23941 cgccgatcgt tcccaccatc gcaaatttca agaagcgtgt gcgttctttt gcgttggtga
    24001 ggatcatgca agccccagtt tttgtttgaa gtacggaatg gaacgaatca gaccttcctc
    24061 aaggctgacc ttgggctccc agttcaaaat ggtttgggcg cgggtgatat cggggcggcg
    24121 gcgttgcggg tcgcgtgcgc tgcgggcatc tacaaaaacg atgcctgcgg tattgcctgt
    24181 cactttgttg atggtctctg caaattgcag aatggtgcgt tcgtgcgggt tacccatgtt
    24241 gacggggaaa tgctcctctg aataaagcaa ttttacgatg ccatcgataa ggtcatctac
    24301 aaaacagaag gagcgggttt gtgaaccatc gccatacaca gtgaggggtt ggcccagcaa
    24361 ggcttgtttg atcatgttcg gcagggcgcg gccatcttcg agatcgagac gcgggccgta
    24421 ggtgttgaag atgcgtacga tgctggtgtt gacgttgtga gagcggtgat aggccatggt
    24481 cagcgattcg gcgaagcgtt tggcttcatc atacacagcg cgggtgccga tggggtccac
    24541 atttcctgtg tacgattcca cttgcgggtg tacttcaggg tcaccataga tttcactggt
    24601 ggatgccagc aggaagcggg cattttgtgc gcgggcgagg ccgagacaat tgtgagtgcc
    24661 aagcgcaccg gccttcatgg tttgaatggg caggttgaaa tagcccgatt tcgattgcgg
    24721 attgggagag gcaggggagg caaaatgcaa gatcgcatcc actttaccgg ggacaaagat
    24781 gtattcagaa acatcacgac ggtagaagga aaaatggtcg ttatctgcca gatgggcaat
    24841 gttatccggg ctgcctgtga caaaattgtc catgccgatg acttcgtgtc catcttttag
    24901 aagatggtcg caaagatgag agccgagaaa cccggctgcg cctgtgatca aaattcgcat
    24961 tagttttatc ctttgattta ggtttatttc caatctattc caacgatcaa cccatcgccg
    25021 gtgacctgat aggctttacc gcttccacta tcgatgagcc acaaattgcc ttgataggtg
    25081 acggcaataa aatcgccaac ctgcccttcg agtggttcgg gcgcccacga cggtgtctgc
    25141 ggttcaatcc cgttcgtgtc ggcgggtggg aacaaagccc tgctgttcga accgtcgcgg
    25201 tccattacca tcaaacgata gcggctggtt tcgctttgct caatgaaaat ggattgcagg
    25261 tatgccacct gataatcttt ttgccttcca tcccatctgg atggcgacac agctggatag
    25321 gaaaacatgc ccgccgcatc tataagggag acagacgctc cattttcgaa cgagatggct
    25381 gccacatcaa aaaaaggtga ctcctcgccg acgatggggg cgggtgccgg cgcatgggtg
    25441 acatagtaca gggtttttcc atccgaaccc caggtgatgg ggggaatcca ggcccaatcg
    25501 ctgtgggtgt tgagcggggt aattcccagc atcggtttca ggtaaccgcc gtcctgatcc
    25561 accagaccga tcccatccgg gcgggcataa gcgaggcggc cgtcttccga atatgcaaac
    25621 gacatgcccc accaaccata cacgccacca ctgttcgctt ccagcatctt gcgcggactt
    25681 ccaccagtga gactgacgcg gtacaagtca ttgtttgcct gccagccggg ggcggtacta
    25741 cgcggctcca cagtggagta tgcaaccgaa tttgtgccgg gaatccattc ggcaaaatgt
    25801 actacgttat aagcctgcag ccagatgggt tttggttcaa tattttttgt gcgaaccgcc
    25861 catagagtat tgatctcctg actggcgggc tttttcgatt tacgagtgaa gatcaggtat
    25921 tcgccatttg gcgataattt gaagatgcgt ccatcgaggt caccagtgct gacaattatg
    25981 cggcggttgg ctgtggagcc ttccatgatc catgcattgc cacccgcaag gtacaccaat
    26041 gtaccgggga tgttcaacgg agtaaaagat gactgtgcgc ctaccattac gatctcgggc
    26101 agggcttccg tcagtaccac ggtttttacc gaggtcttac tgatttcgac ctgattttca
    26161 agaatgcggc gatacgtgac ttccaataag ccgttgacgc cagcctgcac caaccgggtt
    26221 tccccttcgg gcagggtttc attgcggacg atctgccgtt caaacggaac tgtgacctgc
    26281 tcagtctcga agacttcttc gacccgtgtc agggtgatgc tatcgccgtt cgaaagcaca
    26341 gtgtacaaag gcggctcgga acggtctaat ttagcgggga tgatgcctgc gctttgcaat
    26401 gcctgtgaca cggtgctgcc tgccgttacg ctgacctggg ccgcatttcc gtcggcattg
    26461 atggtgactg tgatctgctc accaacctgt ggggaacggc acgaccctgt cagccagatg
    26521 gatatcaata gaaacaggag caggtgcggg cggagtctga ggcggggcat cgggtataat
    26581 ccagccgcta tttgatacgg ccagtcattg tcatcacgcc atctgcgatg gtgatcgctt
    26641 ccaaacgaaa gcctgtggcg gctggcccca atgaacctgt gaaagcctca gcgatcatgg
    26701 cattaatggc ggcgtttaac ccttcgggcg ctgggatggg gccaaggtct actgaagaaa
    26761 cttcgatttt aggcatacct gtggtttcgt caatgccgac actggctgtg ataagcatgt
    26821 tcgcgttgaa ggatccgctg gcgagtttcc cgtacatttg cagttgaccg ttgcgaagca
    26881 ggacttgcgg gtcggtaaat ggcgggtcgg tttgctcttg aagtttgagc gcgataagcg
    26941 aagtgagctg gttttcggtg atctgcaagg tgacaacccc ggtttcggca ccagccgtaa
    27001 aagcctgctc gatttgggtg tacatggttt gtacttcatc tgccgagacg ggtacggttt
    27061 gtgtggggta gtctggcccg ccgacgaaga tattgcaggc cagtgaggcc agcatcaaca
    27121 cggcaaacag gaaagttaag tggtaaattt ttgttttcat ggttttgatt tttgatttca
    27181 ggatactaag cggagcaatt atagcatgag cgactctcaa actacttcgg cctctgtttc
    27241 aactccagag caatcgctgg cggcaaagat cgaggcaatg ctgttcgttt cggcggagcc
    27301 ggtgcccatg gcccagctcg ccgccgcgtt ggatgtgact gcctctgtgg tggagcgcgg
    27361 cctcaaggaa ttggatgaag cccttctaaa ccgtggtttg cgcctgcagc ggaatggcgg
    27421 acgtgtgcaa ctgaccacag ccccggaaat ggccgtgctg atcgaaacat tccttgggct
    27481 tgaagcgacc acgcatctca gccgcgcagc cctggaaacc cttgccatca ttgcctatca
    27541 acagccgtgt acgcgcccac aggtggatgc tgtccgtggt gtgaatagcg atggcatgtt
    27601 gaagagttta ctaagcaagg ggcttgtgca ggaagcaggc cgtacagacg gccctggccg
    27661 cccgatcttg tattccacca ctccggagtt tttacagcat ttcgggttgg ggtccattct
    27721 tgaattgccc ccgctctcgg cccccacaga gcctgataac gagcacagtg agttgctaaa
    27781 aggataaagt aaatttcaaa aacgggatgc aagaggattg catcccgttt ttttgttctg
    27841 aacttttctg aatatgattt atttcatact gtgaacctgc cgcagcagcc ctgccaccat
    27901 ttcttcggcg ggcatggtga gcgattcctt ttggaacatc aatacactta ccacgggcat
    27961 atccaccatc atgtccatga tatccatgcc catcacggtc tgttccgtaa cttgttctcc
    28021 cgacttcgta ttgccgtagc ggtcttcccc agcaaataac ctgcgaacct ggttttcgat
    28081 ctgctcataa aggctgccaa gtacctgccg gccgcgtcgg tctgccatcc actctcgcag
    28141 ggtggattca atatcgagaa tgcagggcag gtccaaggtc gattgaagcg tggttgtgag
    28201 gcggctgcga atgtcagcgg aggaagctcc gatgaggatg tcaaagccgc catcttcggt
    28261 gatccactgc ttgtgttcgg ggtggtagta ggcgaaagcg cggaaatcca aatggatgga
    28321 tacggttttg gtttcgccgg gttgaagttc cactttggca aagcccttta attccttgac
    28381 ggggcggatc agatcggact tttggtcgtg gacgtaaacc tgaaccgttt ccttgcctgc
    28441 gagtttgccc gtgtttttta catctactga aatagtgagg ccatctacat ctttgaaatt
    28501 gttcgaagag actttggcat tgctatattc aaaagtggtg tagctcagac catgaccaaa
    28561 tggaaagagt actggtattt cttttgcatc gtagtagcga taaccaatga aaataccctc
    28621 accatagtga accttccccg ctccgccggg ccagttaatg tgcgacgggg tatcggtaag
    28681 tttgagcggg aaggtttcag cgagtttgcc gctggggttc acacgcccaa aaagaatgtc
    28741 ggcaagagcc gcaccgcccg cctgtcccat catccagcct tcaagcacag cagagacatc
    28801 atcgatccat tcgctcatgg caacgggtgc gccgttgttc aagaccacca cagttttggg
    28861 ttgggctttg gaaactgctt tgatcaacgc gatctgttgc agggtcaggt ccaggtcatg
    28921 gcggtcgtag ccttcagatt ccttgaaggt aggcagggca atgtagagta atgctacgtc
    28981 ggctgattgg gcaagcttca cagcgtcatc gatcatgcct tgttggaagg agttgtcagt
    29041 ggggtagcct tcggcatagg tcaactcggc attttcggca cgcgcctgaa gttctttgta
    29101 cggcatggca accttggtgg ggttgatgtg tgaactgccg ccgccctgga aatgtgctgt
    29161 ttctgccgaa cgtccgatca ctgcaatgtg ctggtgatct ttcagtggca acagcccatc
    29221 attcttcaac aacactacgc cttctgtggc gatcttgctt gctaattcat ggtgagcgtc
    29281 cacgtcaaat gtgccgtttc ctttggcagt ttctttggct ttgaacacaa tgcgcaatat
    29341 gcggcgtaca gattcatcca atgtggattc actgagatca cctgaacgga ctgcttccac
    29401 cacggctttc acgcgtaaat cctgcgggcc gggcatttcc caatccaaac ctgctttgag
    29461 cgcggagacg cgatttcgta ccgcacccca atcggagacg accaggcctt cgaagcccca
    29521 ttcttctttc aggatctcgg tcaagagttg atggtgttca gatgcaaacg ttccattgac
    29581 cttgttgtac gagcacatca ccgtccacgg ttgggcttgt ttgacggctt tctcgaaggc
    29641 gggcagatag atctcgcgca gggtgcgttc gtcaatttca gcgctgatgc tgaaacgttg
    29701 gaattcttga ttgttggcgg cataatgctt aagtgaagtg cccacacctt tggattgaat
    29761 accattgata aagttcgcgg ctaattcgcc cgcaagaaat gggtcttcgg agaaatattc
    29821 aaagttgcgc ccattaagcg gggagcgttt catattcacg ccgggaccga gcagcacgtc
    29881 cacattgagg gcgatacatt cttcggcgag ggcaatgccc atttgcttga tcaggtccac
    29941 gtcccaggtg gaggcggtgc aggatgctgt tggaaagcag gtggcaggca ggcttttctg
    30001 tcccatggaa cggacatctg ctacacgtcg tacaccgtgt ggaccgtcgg agacgatcat
    30061 ctcaggaatc cccaggcgtt caacgggcgt ggttgaccag ttgctggctc cagtacaaag
    30121 ggccgccttt tcttcgagcg tcattttaga aatgatggat ttaatatctt tagccattgt
    30181 ttgttttcct ttttatgact cggtatattc agaaagcaat ataaagccga ctaccaaatc
    30241 gtattcattg gctggtgcaa gttcaaagtc gggaatggca ccattcaagg caatcagtag
    30301 atgcaaagaa gcggttttga gccaaatccc tgccattaaa tcgtcaacgt tgttttcagc
    30361 aagtcttgat ctcttgaaga aggtccgaca aggatttcaa actcgccagg ctcaacaata
    30421 tattcagcct tcgtgttcac gatgcggaag gactcccaag gcagttccac ttcgacggtc
    30481 tttttctcgc caggttggag ttgaacgcgg gcaaagcctt tgagcgcttt attcacccaa
    30541 gtgactgagg tcactacgtc gctgacgtaa acttgcacga tctccacacc ttcacgttga
    30601 ccgatattct ccacgtccac taaaaccttc actgcatcac cttgcgcgag ttttgaagaa
    30661 gacagacgca ggttggagta ggtgtatttt gtgtaactga gtccatgccc aaatgggaag
    30721 agcggttctt gggtcaggtc agcgtagcgg ctgccatgct gtccgcggac ttggctatag
    30781 aagatgggtt gctgcccgac atgcacaggg atggaaatgg tcaacttgcc agagggattg
    30841 agcaaaccga acaa
//
LOCUS       DAOQLC010000043        30608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_474_length_30608_cov_26.738945, whole genome shotgun sequence.
ACCESSION   DAOQLC010000043 DAOQLC010000000
VERSION     DAOQLC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 30608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_474_length_30608_cov_26.738945"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1448
                     /locus_tag="PL000_09520"
     CDS             18..1448
                     /locus_tag="PL000_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89182.1"
                     /translation="MNKKVFTVLLLAVFVLAQFSIASAVVVAGDASITALPDFSSGSS
                     VDLDWAATGMAAGTKNILVWGREYAAVPVAYDWNDCQLIATIVGTGDASGTFTYDFTA
                     TTNLTALGTGDQVEFTLSVDDQTCATAATPAIDSVMGVNYVDDDAPLYIQGNPPSTVL
                     VAPPVDTAACNTFEYWAIGRDATLGPVETAYSGFGDWNPSVTGTFAIPAPMGPAASLL
                     SWKFTFPATASGAWTASIVAEDMVGNANVVDYFRNGVNVVPAELEDCETFTDIAGHED
                     EVYVRYLATLGLISGFADGTFGPDNTLTRAEASALIEISNGYDATLLPTSAPAGCDFT
                     DVAASDWFAGWVWQACDDGLMNGVGGGLFDPNNLLTRGQVVTILNNIYPAYLANPAGF
                     GGYIATNNFALMALPAPAYPYGGYGLRTAAWTDVSVGAYYAIPVQRAYGWGIADGTSD
                     TTFSPDQAATRGEFAKMLYRALSRVN"
     gene            1694..3088
                     /locus_tag="PL000_09525"
     CDS             1694..3088
                     /locus_tag="PL000_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89183.1"
                     /translation="MNKKVFTVLLLAVFVLAQFSMASAATLPGTGSITSIPELSSNAG
                     VNIDWAATGLAGPTQNVLLWGKDSTAVAYMAADCAVISTVAGSGGASGTFTYSFAGVG
                     NGDIWDFTVTVDSMTCGAATVPAVSDVMGSTTIDSVAPYGFVANPPSTEAVPFSSLHA
                     SCNTFELWGVASDRIYTDPDPYSGFNSWNFGQTGSFAPLGPDNSVLSQEITFPATANG
                     AWSFTYNPSDMLGNNWGVGPFYFRNAVAVTPAELAGCADFTDVSGHATEVQVRYLADL
                     GLIAGNPDGSFGVNSTLTRAEASALFEKANGYDASMLPVAPPSAACTFSDVSASDWFA
                     GWVWQACADGFMNGLGGGLFGPADLLTRGQIVTIFNNIDVMGGGTAGSFLDGGGMFNT
                     VLNIEWNNWGTGYHLRVASFSDVPIGQFYTNPVQYAYAVGVADGTSATTFSPDQPVLR
                     GEFAKMLYRALSRV"
     gene            3385..4803
                     /locus_tag="PL000_09530"
     CDS             3385..4803
                     /locus_tag="PL000_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89184.1"
                     /translation="MSKKVFTLLLLAVFMLAQFSVASAVAVAGDASITALPDFSTGVD
                     VDLDWAVTGMAAGTKNVLVWGRVNAAVPVAYDWNDCQLIATINGTSDASGTFTYDYTA
                     TTNLTALSSGDIVEFTLTVDDQDCATAATPAIDSVMGSNYVDDDSMDGDYFNPPSLSI
                     ADFDSTPVSCNTFEMWAVVSDKVTVPAGDAYSGFGSWNPTTTGTFVPAPIGADSALFS
                     WVFTFPSTASGAWVLEFNPTDAAGNDWGVDTFRDGGAPVDVVGAAELEDCADFTDTAG
                     HEYEVYIRYLADLGLISGFADGTFGPDNNLTRAEASTLFEIANGYDATTLPTSAPAGC
                     DFTDVAASDWFAGWVWQACADGFMNGVGGGLFDPNNLLTRGQVVTIFNNIYEMGGGTG
                     DYLDFTNGNVLSQDWTSWENPYLREAAWTDVSVGAYYAIPVLRAYGIGVADGTSATTF
                     SPDQAATRGEFAKMLYRAISQQ"
     gene            5000..6415
                     /locus_tag="PL000_09535"
     CDS             5000..6415
                     /locus_tag="PL000_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89185.1"
                     /translation="MSKKVFTLLLLAVFMLAQFSVASAVAVTGDASITALPDFSTGDD
                     VDLDWAATGMAAGTKNILVWGRVSAAVPVAYDWNDCQLIATINGTSDASGTFTYDYTT
                     TTNLTALSSGDIVEFTLTVDDQTCAAAATPAIDSVMGSNYVDNDSMGGDYFNPPSLIA
                     SFSNIPVSCNTFEMWAVVSDKVTVPAGDAYSGFGSWNPTTTGTFVPAPIGADSALFSW
                     VFTFPSTASGAWVLEFNPTDAAGNDWGVDTFRDGGAPVDVVGAAELEDCADFTDTAGH
                     EYEVYIRYLADLGLISGFADGTFGPDNNLTRAEASTLFEIANGYDATTLPTSAPAGCD
                     FTDVAASDWFAGWVWQACADGFMNGVGGGLFDPNNLLTRGQVVTIFNNIYEMGGGTGD
                     YLDFTNGNVLSQDWTSWENPYLREAAWTDVSVGAYYAIPVLRAYGIGVADGTSATTFS
                     PDQAATRGEFAKMLYRAISQQ"
     gene            6539..7696
                     /locus_tag="PL000_09540"
     CDS             6539..7696
                     /locus_tag="PL000_09540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC89186.1"
                     /translation="MKKLRVALYCPDHHLSYDGSTPDKQGVGGGVMVRIRLAAALAKL
                     GHQVTMICNCPRAAVFDGVQYLPLAQVRSIDVDVLILSSSGGDLDLRPALDLDIKASL
                     RLIWVHGTPLLKGLHEIPFDYVCSPSNFIRQWAIQEWGVPREKTFVSYHGVDPAFLQE
                     PGLFNRRERNLFKLVYASHPSKGLDAALGVLRILRAQDARFELHIYGGNRLWGGAEQA
                     PAQEDGVFYHGMVGQRELSRELRTSHIALNLQDRLEPFGLSLIEAMASGNIVVASPVG
                     AYPEIVRDGYDGFLVEGNHMVEETWQKVAQMALTLVSAPDFSSTIRHNAQAAVRNWET
                     VARTWVGHWEWAMHVSERRMWADPCPDCGGLQLPLADGYHCASCGLYLRKG"
     gene            7705..8532
                     /locus_tag="PL000_09545"
     CDS             7705..8532
                     /locus_tag="PL000_09545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNC89187.1"
                     /translation="MLYKLYRKALNLYQQNPESLLARLVRMEINWLVKTLQRRQQFHF
                     PARATGGWWWIERFRFEILMRWLEVESVERVRKVVQPGMTVVDIGAHIGYYTRLLADL
                     VGPSGRVLAFEPNPENFSVLQKNMSLPRYRHVELFNYAVGERESILPLYVSPGNSNHS
                     LIEGYTKAEGVIEVRCVDLDSFLQERGIEQISFVKSDTEGAEPLVLKGMKNIAARSTN
                     LHLLMELNPTALRCGHVEPEQFLESIRSMGFEIEMITSELSAKESDANMLCSKVLKK"
     gene            8753..10966
                     /locus_tag="PL000_09550"
     CDS             8753..10966
                     /locus_tag="PL000_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNC89188.1"
                     /translation="MIQLFKKTSIILLVILIVLAAQPSIVAHAAPPAPIEITLYQPDG
                     FAFQARAWGDEWSNGMETLDGYTILQDGQSLFWVYAAEPTHDSLRPSTLIVGRDDPAA
                     AGLPLHLRPNVNRPAGSPPAGFQPIPEDSINVGTQRVLVLLAQFQNQPSLGTDAAYWN
                     DRIFGPALSVKKYYKEVSYGQLNLLPAIENYGTSRDGIVGWITLPYNHPNSGGNVSDH
                     NRQITRDAILASAPYVDYTVYDTNNDGYISNKELHIIVVVAGYETAFASTSSCKPGIW
                     AHYWGFEGGKVPAPVVYGKTIGEISAGGGYVQVGEWHCADLPGLTPGYPATMGVIAHE
                     FGHSLNLPDLYDTGGGSSGIGVWGIMGMGTWNTTGTQLGDLPAHHDAWSKWYEGWITP
                     LQLRGLSAGQPIPQAESNPTVFQLLDNPNGLDWKFKSNSGTGEYFLVENRQQTGFDAG
                     LPGCGLLIWHIDESVPFDNSANAGNPRRLVYLVQADGTDELGRGRDTGDAGDPFPGSR
                     WRQAFSASTTPNSNLYDGSPSSVNVLNISGCASTMTADFLTPGDGTFADIAPDYWAVD
                     SIEKLYQNGITQGCGVYPLIYCPESSTTRAQMAIFLLRSKHGSSYTPPAATGTAFADV
                     PADHWAAAWIEQLVTEGITAGCGDGNYCPESEVTRAQMAVFLLRSKYGGSYTPPAATG
                     TAFTDVPADYWAAAWIEQLVAEGITAGCGDGNYCPESAVTRAQMAVFLVKTFALP"
     gene            11041..11964
                     /locus_tag="PL000_09555"
     CDS             11041..11964
                     /locus_tag="PL000_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89189.1"
                     /translation="MATWSEDLFSIIQMARSSSQTGMKACPKNPFGWELQSQKPRESL
                     LPHFGVRAAGSWNFILTKSTRQNKDVLAAVLGGENVEWIAEEELDTYQDLKITDKLMD
                     YLGIADIDYLSARLLFMSGFPIVAMAKAAEALEKLFKILIVMITRIYSQVELTDDQLK
                     KDYGHDLGKLLKGYNKIAPESVRLIGDTTILDNLRKAYNLRYTGRDKEINLEFSLEEF
                     DKWYVLLRNLIVRNTPKELAIEVRQFGTFHGKLYEAGMEKILSQYRTLRPGDILRYQN
                     AMFDKLDVDLQCPLCQYRSGEIREPTLDDRT"
     gene            12148..14922
                     /gene="csaB"
                     /locus_tag="PL000_09560"
     CDS             12148..14922
                     /gene="csaB"
                     /locus_tag="PL000_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HNC89190.1"
                     /translation="MDKNNKKTILVAGYYGFGNVGDEAILSAILTDLRKQREGLEFIV
                     ISSNPEETAASHNVRSVHWKEINALMDAARECDLIILGGGGIFQDYWGTPAGTHLTEF
                     HWGISYYSSIGLLASLFDKPFMIYSVGIGPLLTDEGKRWTRLAFELADAATVRDVESR
                     DLLASLGFPKKQILIKPDPALNLSPKLKAAAEILQAEGISPKERPMVGVCIRNWTEGE
                     KGGAWKQELASALDAFLERHNAQIVFIPFQVGEHELENDYTVSVEVISMMQNQERTKV
                     LPQAYSPEVVEGLIARCRVVIGMRLHSLVFAASVGVPSVALIYDPKVRNLMKFLGLSE
                     YALDLQTMNVDSLSDTFHYLWMDQKQVRASLAEQGQKFKQLVQTNTTLALKLLDQKTK
                     RIEKPVALQLIRDLAVKQTGAFFVREKEAQALTAEVASLNQQVADQLARIAINEYEID
                     RIQKIVDYQQYQLHEMVHSTSWKIALIFQKIRTTLIPLGSRREKLVRFVFHWFVRKPL
                     SLISRIRTSIRVNGVFGAILKGFVAIWVALTYPIKKAIFKQRYERELKELEAIIAGHN
                     GFFDLFHIPMGWNTMLFQRFQHVSLQSAKLGGIAIYGGHPTVDKDIFVYKKAAENLYV
                     FKATDPQITERILQALEKKGNSTVLRIESIDLVTTAEDVANFLRRGFRVVYEYIDEIT
                     SDITGNVPELVRDRHQALLRNEAVFVVATSDQLYEEVARHRAKNFVLSTNGVDLENWR
                     IPKGEPPVDMKSAFTGNTIVAYHGALAKWLDYDLLRMIADEGSYELVLIGHEHDDAFE
                     KSGLKGHPRVHFLGSKPYSQLNQYAIHYGVTILPFKKSNLTEAVSPVKIFEYMAALKP
                     IVTTDLRECKKYKSCLTAETNEEFMQQLKRASELRSDEEYLKVLDTEANENSWEHKTI
                     EILHMTGVKL"
     gene            14970..16235
                     /locus_tag="PL000_09565"
     CDS             14970..16235
                     /locus_tag="PL000_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNC89191.1"
                     /translation="MPEPLRRRLSMLSQRIFRPRSAPAMPEPAKTKMYEQYTKQVLAI
                     PDKTDDYVDLASDAYVPQAGDPLLLAYYLPQFHPTPENDEWWGRGITEWHNVAKAVPQ
                     YVGHYQPRLPGELGSYDLRLKENIARQIELAKSHGIHGFAYYFYWFDGRRLLDKPLDM
                     FLNNPDLDFPFCLCWANESWTRRFDGSSGEVIMYQNESVESYKSFIESIKPYAADKRY
                     IRVDGRPVLIVYRPSFVPDCSNVLAHWRAYCEKEGLGNPYIIAVKENTFDGDLLALGF
                     DAQSEFHPGTVFRYCKNITNEINFMRDDFGGIVLDYVDLVKNKQYFKFQAKKLHRAIM
                     PMWDNTPRRNNTPMIFEGASPALYKEWLIDILREARSSKELDAPFIFVNAWNEWGECA
                     YLEPDRKYGYAFLRATREAIEETRKAEGE"
     gene            16281..17276
                     /locus_tag="PL000_09570"
     CDS             16281..17276
                     /locus_tag="PL000_09570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC89192.1"
                     /translation="MNAPLISIVLVAWNSAAYMPRCFQHLLAQTVQDFEIIVVDNGSK
                     DDGCVGLQEKYPTLALRVERLETNHGFSFANNLGAQLATGKWLTLLNTDAFPEPDWLE
                     RLLEAAQMHPNAFFSSRQLQANTPELLDGEGDVYHVSGLAWRNGYGSRTYPLDETREV
                     FSACGAAAFFPREKFLSVGGFDEDYLSYHEDVDLGFRLRLHGLKCFVVPRAVVHHVGS
                     VTFGKKSDFSIYHGHRNLVWTYAKDMPSVLFWFYLPLHIFINVFFLFYFTAKGQGRTI
                     WRAKFDALRGLAPMLKKRGAIQKNRKASIAELHRAMSRNWLAPFLTFLKRNTENS"
     gene            17273..18169
                     /locus_tag="PL000_09575"
     CDS             17273..18169
                     /locus_tag="PL000_09575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC89193.1"
                     /translation="MKPDISICITNYNAKDFLRDCLRSIYGTTDTLTFEIIVVDNFSG
                     DGGPEMIRSEFPHVKLLTNEGNTGFTRPYNQAMRLAQGRHVVVLNPDTVIFPKAFAEL
                     VGFLDSHPEVGIVSPKVLNRDGTLQKQCRRSEARPWDAICYFSGLSRLFPHDKRFAGY
                     LMTYLPEDLTHEAEAVSGSCMVVRREVVEQVGYFDEDFFAYQEDSDYCRRTRLAGWKI
                     FYVPTAQITHYGGEGGSKVQPYRTIIEWHRSYYLYFRKHFAKDYFFLFNYLYYLMMLT
                     KLGYALFTNLFRRKKIVSTPKP"
     gene            complement(18244..18942)
                     /locus_tag="PL000_09580"
     CDS             complement(18244..18942)
                     /locus_tag="PL000_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC89194.1"
                     /translation="MSASSAMQRYAAEIYRLQQDHEQVPLSLLSTHVESSAQAIASMV
                     KRLQKNGYLVHEPYRGVRLTPTGEKIAMPSLRRHRLTEVFLVKVMKYDWATAHQLADT
                     FELGVNDMIEDRMDELAGRPTRCPHGEPIPTKDGVMPVVRDMSLIDVPSGSDCVISRV
                     RTHDFEKLTYIGELGLVPGTSFHLLSCAPFKGPLRLNMKPHDHVVGYELAASLWVEVT
                     KEGEGNKLPAQKNN"
     gene            19108..19650
                     /locus_tag="PL000_09585"
     CDS             19108..19650
                     /locus_tag="PL000_09585"
                     /inference="COORDINATES: protein motif:HMM:NF017987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChuX/HutX family heme-like substrate-binding
                     protein"
                     /protein_id="HNC89195.1"
                     /translation="MFQTIQPLNLDLTVVERQKTIRDAYNTDRSQMTLMLAHQLRIPE
                     VEIVRALEGDSAHELDFSRWQEIVCAFETLGNVHVIVSNGAATIEAYGQFGNLTNSDG
                     FLNVRTKSLDMHIRSWELDSVFAFRKPSHLDGIETLSFQFFDRQGAAAFKVFLNFSGK
                     VSAPALVERYDALIRQFGKK"
     gene            19818..21122
                     /locus_tag="PL000_09590"
     CDS             19818..21122
                     /locus_tag="PL000_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HNC89196.1"
                     /translation="MFIKRLQLALIHTAVAITLVPINSTLNRVMIFDLGVSKTLFTLL
                     AIFPYLLSPIQVAIGSFSDRHPIMGYRRTPYILAGLLLCVLGVAIAPQIAVLVSENFT
                     LGIMVGIFAFGLWGMGYNLSAVSYLSLASELSGEKGRGKTVATMFTIMVIGLIATGIS
                     LSRMVTVYDPETLSRAFLIVAASALTLGLLGLFKLEPPFGRSDQTPRSETYTVKEMTA
                     AITSNPVAKIFFAYLLLLLAAILGQDVLLEPFGAQAFGMSLEQTSRIVSISSTFTLVA
                     FIVAGVLEGRVKKKYIAQMGNLGALIGFLIIVVSGLTGSLTAFYLGITLLGFGTGIST
                     VANLSLMFDLTVPEKVGLYIGAWGFSNGLSRLVGLLMAGVVADLATQMTGSALSGYLV
                     VFSLEALMLLAAAVMLYRIDVGAFQKQAHEPSYVEKVALSVE"
     gene            21125..21742
                     /locus_tag="PL000_09595"
     CDS             21125..21742
                     /locus_tag="PL000_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC89197.1"
                     /translation="MTDEWLSLSDAAKILGVHPGTVRLWSDKGSLPTHKTQGGHRRYR
                     RSEISLWAESNHRSNAIEPEGMMNEVVKNVRLQISEGRLQAESWYQKLDDDARTQYRM
                     SARSLFHGLMNYVATHGAEAESESYAIGYEYASRAHRYSLNYVDAAKAFLFFRNTLIE
                     SVIKVYGEANVPSGQTAEMFQKMHVFTDEILISLLQTYQSLNASR"
     gene            21826..22536
                     /locus_tag="PL000_09600"
     CDS             21826..22536
                     /locus_tag="PL000_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89198.1"
                     /translation="MQAVTHYIPIFTTVISFSFTVALYKHWQRKPEARYLMWWTIGAF
                     CFGIGTLTESINTLVGWSVWNMKAWYISGALLGAFPLAQGTVYLLLKKKTADFLAWFF
                     VVYIAVASVAIFLAPVDMSLVDPARLTGKVMTWTWARMFSILPNTYAMIFLVGGAAWS
                     AIQYARKLDSGSRVLGNWLIAIGALLPGIGGSFTRFGYVEVLFVTEFIGISLIWAGYR
                     AMTQTRTVSIHTAQAAAD"
     gene            22656..24134
                     /locus_tag="PL000_09605"
     CDS             22656..24134
                     /locus_tag="PL000_09605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC89199.1"
                     /translation="MPIPSKIGRYEVKSELGRGGMATVYRAYDPSFDREVAIKVLPRE
                     MLHDPQFRGRFDRELKIVAGLEHPSIVPVYDVGEEDGQPYFVMRYMTGGSLSDQIALG
                     KISVQDTARIVERIAMGLHYAHKKGIVHRDLKPDNILFDNNGDPFISDFGIAKLTESS
                     SGSLTGSGVIGTPAYMSPEQAKGEPIDGRSDIYGLGVIIYQMLSGQQPYNADTPMGVV
                     VKHITDPVPEILNMIPSLPIEVDGIIKMCMAKDKTRRYANAVELAKAMNMVAFGNEGK
                     AGLSNTGFRTVLPGTSTLQSPRARTGLAVAGVVLLVVVVGGFLLRNQLFTQPEATSTP
                     TLTAVVFTSTVEEVPTEKPTLTSEPPTETVASPLPFAPACAEGITIPVPEVRETNKAC
                     VTKIPYTSVSIPEGAEFESLSPDMTCAIETTSDGKSIVACTGRQLYSYELKVCTPPVV
                     ANEDLNKCESGSLFEAANQCCVPTPKDGAGCTTFKIDLRSCQ"
     gene            24273..25097
                     /gene="rsmI"
                     /locus_tag="PL000_09610"
     CDS             24273..25097
                     /gene="rsmI"
                     /locus_tag="PL000_09610"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNC89200.1"
                     /translation="MGTLFLVATPIGNLEDISPRALRILKEVQLIAAEDTRHSGVLLK
                     HFGIETRLTSYFEHNKIHKLDQILSALAEGDVALISDAGTPAINDPGYELVRAALASN
                     FDVVPVPGPSAPIAALTVSGLPTDSFLYLGYLPHKSSERLGFVGQVSNLPYTLIFLET
                     PHRLLDSLEDILAVLGDRRICIAREMTKRYEEYWRGDISGALEYFKSKDPRGEFTLVV
                     EGKKKEESSKWEESELMDAIRKELQTGRSAKEISADLAGQSGWNKKDVYALVNLVK"
     gene            25094..26170
                     /locus_tag="PL000_09615"
     CDS             25094..26170
                     /locus_tag="PL000_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89201.1"
                     /translation="MNEQNSPTIPPLLGSFSQRLLYWIFIPVAPIVNFSFVSAMLPLW
                     QTGKFSDYMLLMLSPEAAALTLPLLGYSVISYLMLISDEERQSESLVIRFGIYGGVLL
                     SFHYSLLTALSMDLSPVLFGFVFFIFLPLLIPKLRAWLQNEKVFRALIFAAFAILVLW
                     HIVAAIFDYSTAPVLLLVILVPMSAPFWCFFIALQAARWLWKYHEPKLTHAHGFGLFT
                     WLSAYGVALYYNITKMYELYATLPTQPPNCYIATAAANGHSAFVGSQEIRLENGISMR
                     VNRQLQNLKCAELTLMVIAPHLHKILRSVYDLFGKALARRIQNPLLADAAYLSLKPFE
                     WTAVAVLELIVPNWDDLASKIYIR"
     gene            26265..26639
                     /locus_tag="PL000_09620"
     CDS             26265..26639
                     /locus_tag="PL000_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89202.1"
                     /translation="MEYLTAIFSSILPQLPSLFLHAVGLGFSISRRKEYPRTALAAGI
                     YFGLALLIGLATQVYVVLPMYMVDQGGSTQDIGSIFTTLTFICVPFRVVMEVALLYAI
                     FAPRNSNSSDPNQPIFMGDKNG"
     gene            26632..28116
                     /locus_tag="PL000_09625"
     CDS             26632..28116
                     /locus_tag="PL000_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89203.1"
                     /translation="MAEKKPEEKKDEKKDEKPAPKDNLVETKHSVKIGGKTIKYTVTT
                     GTMVVKEEVWDKDKEAEVDKPRAQMFFVAYTLDGVKDRSKRPITFSFNGGPGSSSVWL
                     HMGVLGPRRVVLTDEGEMPPPPFKLTDNAFSILDETDLVFIDPMSTGYSRPVEGEKSR
                     EWHTFSKDIASVGDFIRLYSTRYNRWLSPKFLAGESYGTTRASGLSGYLQERHGMFLN
                     GLILISAVLDFTTIDFNLNNDLPYILFVPAYAATAWYHGKLSFKASLKEVVREAEEFA
                     KGEYATALLKGAALTPEERASVVEKLSRYTGISKEFIERTNLRIYDQHFFKELLRERG
                     KTVGRLDSRFTGIDRNGVTAMPDYDPLFAQVNGPYTATLNDYLRTELKFETDLPYEIL
                     SDKVFLNWSYEYFQNQYVNVAETLRASMTFNKYLKVFVANGYYDLGTPYFATEYTFDH
                     LGLDESLRKNISMGYYEAGHMMYVHIPSLKQLKKDLSKFIKDAS"
     gene            28203..29261
                     /locus_tag="PL000_09630"
     CDS             28203..29261
                     /locus_tag="PL000_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC89204.1"
                     /translation="MNLDDLDLFKKIDTRNMLAQIDGLPNQLQTAWELGQTLPLPAFK
                     DIQTIVISAMGDSAVAAELVIASVSSSIRLPVALHRGYGLPAFASGPQTLVVCISHSG
                     NSEEVLDVFEAAQKNNCTVMVISTGGEIAMRAADKNIPAWKFDSNGVVDTAAVAYPFG
                     LLLALFARLGLIPDPASDVAEAVAMMKRSQQHITADVIAAKNPAKRYAGQLVGRWVTF
                     VGTENLAPVARRWKMQINQLAKAGANFEIIPESTHNTLIATINPNPTLNAHTMTLFMK
                     APSDHPRNKLRSDLMRQAFMLEALNTDVIEARGDSVIAHLWTLMIFGDYMAYYLAMAY
                     GADPSEEDAFVNFKRSLS"
     gene            complement(29293..29865)
                     /locus_tag="PL000_09635"
     CDS             complement(29293..29865)
                     /locus_tag="PL000_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC89205.1"
                     /translation="MSPTLKEEEKLDPRVKRTRNLILTSFTSLLAEKGFDSISVQDVA
                     NKAEINRVTFYAHFSDKYALLDYAIQKTFWTEIEKRSLNMCAYSADNLRNLIQVVCEF
                     LVKSNSGCAQPSQQFESLAEATTKKQIFDLLSHWLNKPQLPTEIPATVATWAIYGLAT
                     HYSHMKKRPVLEKFVDEALPLVAVNLEQFA"
     gene            30002..30373
                     /locus_tag="PL000_09640"
     CDS             30002..30373
                     /locus_tag="PL000_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNC89206.1"
                     /translation="MNIALWIVQGLLAVLYGMAGSMKTFRPDFYRNNPQTESWAKERS
                     DWGLRQIGIPEFLGALGLILPMVTGILPWLTPLAAVGLTVIQLLALFAVHLPKKEPIV
                     PNIILGALSVFIVVGRWGLFS"
BASE COUNT         6891 a         8153 c         7896 g         7668 t
ORIGIN      
        1 tttataaagg agagaacatg aacaagaaag tatttacagt tttgttgttg gctgtgtttg
       61 tgctggccca gttcagcatc gcctccgctg ttgttgtggc cggagacgct tccatcactg
      121 ccctccccga tttttcgagc ggctcaagtg tggacctcga ttgggctgcg accggtatgg
      181 ccgcgggcac caagaacatc ctcgtttggg gtcgtgagta tgcggctgtt cctgtggcct
      241 acgactggaa tgactgccag ttgatcgcca ccatcgtagg cactggcgat gcgagcggca
      301 ccttcaccta tgatttcact gccaccacca accttaccgc gttgggtact ggtgatcagg
      361 ttgaattcac cttgagtgtg gatgaccaga cctgtgctac agccgccacc cctgcgattg
      421 actccgtcat gggtgtcaat tatgtggatg acgatgcccc gctttatatt cagggcaatc
      481 ccccctcaac agtacttgtt gcaccccctg ttgacacagc tgcctgcaat accttcgagt
      541 attgggcgat cggccgtgat gccactcttg gacctgttga aacggcttat tccgggtttg
      601 gtgactggaa tccctcggtg acgggcacct ttgcaattcc cgcgccaatg ggccccgccg
      661 cttccctgct ctcctggaag tttaccttcc ctgccactgc ctctggtgca tggactgctt
      721 ccattgttgc ggaagacatg gttggaaatg ctaacgttgt ggactacttc cgcaatggtg
      781 tgaatgtggt tcctgctgaa ttggaagact gcgaaacctt cactgatatt gccggtcatg
      841 aggatgaagt gtacgtacgt tatctggcga ccctcggcct gatcagcggc ttcgccgatg
      901 gcaccttcgg ccctgacaac accctcaccc gcgcggaagc ctcggctttg atcgagatct
      961 ccaacggcta cgatgccacc ctgttgccga cctcggctcc tgctggttgt gacttcaccg
     1021 atgtggctgc ctccgactgg tttgctggct gggtctggca ggcttgtgat gacggcctta
     1081 tgaacggtgt gggcggcggt ctcttcgacc cgaacaacct gctcacccgt ggacaggtcg
     1141 tgaccatcct caacaacatt tatcccgcat accttgccaa cccggctggt tttggtggat
     1201 acattgcaac taataatttt gcattaatgg ctctccctgc ccctgcctat ccttatggtg
     1261 ggtacggcct tcgtaccgcc gcctggactg atgtttctgt gggtgcatac tatgccatcc
     1321 cggtgcaaag ggcctacggt tggggtattg ccgacggcac ttctgatacc accttctcac
     1381 ccgatcaggc tgcaactcgc ggcgaattcg ccaagatgct gtatcgcgct ttgagccgcg
     1441 tcaactaatt tttctttgct cgtgtaattt gctttctgga gcggacccgg taccgggtcc
     1501 gctctttttt gtggaacagg ttgcaggtcg ggtttgatgc ggggatataa acaaaagtca
     1561 tgcccttacc aatttcccat gtttactggt tgactttgaa ttgatacatc catagaatag
     1621 ctacattcag gatcgtcatc gtctgaatca acgtgaattg gatgacaacc aatattttta
     1681 taaaggagag aacatgaaca agaaagtatt tacagttttg ttgttggctg tatttgtgct
     1741 ggcgcagttc agcatggctt cagccgccac attgcccggt acaggttcga tcaccagtat
     1801 tcccgaacta tccagcaatg ccggtgtgaa cattgactgg gctgctaccg gcttggctgg
     1861 tccaacccaa aatgtgcttc tttggggtaa agattctact gccgtggctt acatggcagc
     1921 tgattgtgcc gttatatcca ctgttgctgg cagcggtggt gcatctggca cgtttaccta
     1981 ttcattcgct ggtgtgggta atggcgacat ctgggatttt acggttacag ttgatagtat
     2041 gacctgcgga gccgctactg tccctgccgt ttccgatgta atgggcagca ccaccattga
     2101 tagcgtggct ccttatggct tcgttgccaa cccgccttcc accgaagctg tgccgttctc
     2161 cagcctgcat gcctcctgca atacctttga gttgtggggt gtggcttctg accgcatcta
     2221 caccgacccc gatccatatt ccggcttcaa ttcatggaat tttggacaga ctggttcctt
     2281 tgccccgctt ggaccggata acagcgtact ttcacaggaa atcaccttcc ctgccacagc
     2341 caacggtgcc tggtctttca cctataaccc gtcagatatg cttggcaata attggggtgt
     2401 tggcccgttt tacttccgca atgcggtggc agttacccct gctgaacttg ctggttgtgc
     2461 cgactttacc gatgtttccg gccatgctac agaagtgcag gtgcgctacc tggccgacct
     2521 cggcttgatc gctggtaacc cggatggcag ttttggtgtg aacagcaccc tgacccgcgc
     2581 tgaggcttct gctttgttcg agaaggccaa tggctacgat gcttccatgc tgcctgtggc
     2641 tccgccttct gcagcttgta ccttctctga tgtttccgct tctgactggt ttgctggctg
     2701 ggtctggcag gcttgtgcgg atggcttcat gaacggcttg ggtggtggtt tgttcggacc
     2761 tgctgacctg ctcacccgcg gccagatcgt caccatcttc aataatattg atgtgatggg
     2821 cggcggtacg gctggctcct tcctcgatgg cggtggtatg ttcaacaccg tgctcaacat
     2881 cgagtggaat aactggggta ccggttacca cctgcgtgtg gcttccttct ccgatgttcc
     2941 cattggacag ttctacacca atccggtgca gtatgcatac gcagtgggtg tggctgatgg
     3001 caccagcgcc accaccttct cacctgacca gcccgtgctg cgcggtgaat ttgccaagat
     3061 gctctaccgc gctttgagcc gcgtgtaaga aacaatcgat aaaatgtaat cccgggcgga
     3121 ctcgcaagag tccgcccttt tttgttggca gaagaattcg cgagagtggc taagtccgtg
     3181 ggctaatcac aaagacgcca agccacgaag ccaccaagaa tccttgtgcc ttcgtggcaa
     3241 tcaaagccca tttttttagc cactctcgaa ttcgcatcca tattgacttc gaaatgtgcc
     3301 tcgcataaaa taggataatt caggtcgcca ttgcctgaaa actatattgc tgagtggcat
     3361 atattttttc ccaaaggaga gaaaatgagc aagaaagtat ttaccctttt attgctggct
     3421 gtgttcatgt tggcccagtt cagcgtcgct tctgctgttg cagtggcagg agatgcaagc
     3481 attacggcac tgcctgattt ttcaaccggg gttgatgtgg acctcgattg ggcggtgact
     3541 ggcatggctg ctggtaccaa gaacgtgttg gtgtggggtc gtgtgaatgc tgcggtaccc
     3601 gtagcctatg actggaatga ttgtcagttg atcgccacga tcaacggcac cagcgatgca
     3661 agcggcacct tcacctacga ttacaccgcc accaccaacc tcactgcact gtcttccggt
     3721 gacatcgttg agttcaccct gaccgtggat gaccaggatt gcgccaccgc tgccacccct
     3781 gcgatcgact ctgtcatggg ctccaactat gtagatgatg attcaatgga tggcgattac
     3841 ttcaacccgc cctctttgag cattgccgat ttcgatagca cccccgtctc ctgcaatacc
     3901 ttcgagatgt gggctgtggt gtcagataaa gtcaccgtcc ctgctggtga tgcatactct
     3961 ggctttggtt cctggaaccc gaccacaaca ggtactttcg tccctgcccc gatcggtgcg
     4021 gatagcgccc tgttctcctg ggtgtttacc ttcccctcca ctgccagcgg tgcgtgggtg
     4081 ctggaattca acccgaccga tgcggccggg aatgactggg gtgtcgatac attccgcgat
     4141 ggtggtgcac cagtggatgt ggtgggtgcc gctgaattgg aagactgcgc tgacttcact
     4201 gacaccgctg gtcatgaata cgaagtgtac atccgctacc tggctgacct cggcctgatc
     4261 agcggcttcg ccgatggcac ctttggtccc gacaacaacc tgacccgcgc cgaagcgtcc
     4321 actttgttcg aaattgccaa cggctacgat gccaccaccc tgccgacctc ggctcctgct
     4381 ggttgtgact tcaccgatgt ggctgcctcc gactggttcg ctggctgggt ctggcaggct
     4441 tgtgcggatg gcttcatgaa tggtgtgggc ggcggcttgt tcgacccgaa caacctgctc
     4501 acccgcggcc aggttgtgac catcttcaac aacatctatg aaatgggcgg cggcaccggc
     4561 gattatctcg atttcaccaa tggcaacgtc ctcagccagg attggaccag ctgggaaaac
     4621 ccgtacctgc gcgaagccgc gtggacggat gtctccgttg gtgcgtacta cgccatcccc
     4681 gtcctgcgcg cctatggcat tggtgtggcc gatggaacca gcgccaccac cttctcgcct
     4741 gaccaggctg ccacccgtgg tgaattcgcc aagatgctct accgcgccat cagccagcaa
     4801 taacaagctt tctgttcgaa tgtaatctga gcggacccgt acgggtccgc tctttttatt
     4861 gttttcccag tcactttggg ggagttctgc atccataatt gacttaggaa tttccctcat
     4921 ataaaataaa agaattcagg tcgccattgt ctgaagttgt ttttaccgaa tggcatccat
     4981 attatttgta ggagagaaaa tgagcaagaa agtatttacc ctattattgc tggctgtgtt
     5041 catgttggcc cagttcagcg tcgcttctgc ggttgcagtg accggagatg cgagcattac
     5101 ggcattgccc gatttttcaa ccggagatga tgtagacctc gattgggcgg caaccggcat
     5161 ggccgctggc accaagaaca tattggtgtg gggtcgtgtg agcgcagcgg ttcctgtagc
     5221 ttacgactgg aatgattgtc aattgatcgc cacgatcaac ggcaccagcg atgcgagcgg
     5281 caccttcact tatgattaca ctaccaccac caacctcact gcgctgtctt ccggtgacat
     5341 cgttgaattc accctgaccg tggatgacca gacctgtgct gccgcagcca cccctgcgat
     5401 cgactctgtc atgggttcca actatgtgga taatgattca atgggtggcg attacttcaa
     5461 cccaccctct ctcatcgctt cttttagcaa catccccgtc tcctgcaata ccttcgagat
     5521 gtgggctgtg gtgtcagata aagtcaccgt ccctgctggt gatgcatact ctggctttgg
     5581 ttcctggaac ccgaccacaa caggtacttt cgtccctgcc ccgatcggtg cggatagcgc
     5641 cctgttctcc tgggtgttta ccttcccctc cactgccagc ggtgcgtggg tgctggaatt
     5701 caacccgacc gatgcggccg ggaatgactg gggtgtcgat acattccgcg atggtggtgc
     5761 accagtggat gtggtgggtg ccgctgaatt ggaagactgc gctgacttca ctgacaccgc
     5821 tggtcatgaa tacgaagtgt acatccgcta cctggctgac ctcggcctga tcagcggctt
     5881 cgccgatggc acctttggtc ccgacaacaa cctgacccgc gccgaagcgt ccactttgtt
     5941 cgaaattgcc aacggctacg atgccaccac cctgccgacc tcggctcctg ctggttgtga
     6001 cttcaccgat gtggctgcct ccgactggtt cgctggctgg gtctggcagg cttgtgcgga
     6061 tggcttcatg aatggtgtgg gcggcggctt gttcgacccg aacaacctgc tcacccgcgg
     6121 ccaggttgtg accatcttca acaacatcta tgaaatgggc ggcggcaccg gcgattatct
     6181 cgatttcacc aatggcaacg tcctcagcca ggattggacc agctgggaaa acccgtacct
     6241 gcgcgaagcc gcgtggacgg atgtctccgt tggtgcgtac tacgccatcc ccgtcctgcg
     6301 cgcctatggc attggtgtgg ccgatggaac cagcgccacc accttctcgc ctgaccaggc
     6361 tgccacccgt ggtgaattcg ccaagatgct ctaccgcgcc atcagccagc aataacaagc
     6421 tttctgttcg aatgtaatct gagcggacct gtatgggtcc gctctttttt atggcaattg
     6481 acgcatccct caatcaacta taaaatgctt ctgtcacgat caagatggga gtgtctcttt
     6541 gaagaaatta cgtgttgctt tatattgccc cgatcaccat ctttcctatg atggcagcac
     6601 cccggataaa cagggagtgg gtggcggtgt catggtgcgg attcgtcttg cggcggcgct
     6661 ggcaaaactg gggcatcagg tcacaatgat ctgcaattgt ccgcgtgcgg cggtgttcga
     6721 tggggtgcaa tatcttccgc tggcacaagt gcggagcatt gatgtggatg tgctcatcct
     6781 cagcagcagc ggcggcgatc tggacctgcg cccggccttg gatctggata tcaaagcgag
     6841 tctgcgcctg atctgggtgc atggcacacc cttgctcaaa ggcttgcatg agattccatt
     6901 tgattatgtc tgttcgccga gtaatttcat ccgtcagtgg gcgattcaag agtggggcgt
     6961 tccgcgtgaa aaaacatttg tcagctatca cggcgtggac cctgcctttc tgcaggagcc
     7021 aggtcttttc aaccgcaggg agcgaaatct ttttaaattg gtgtatgcaa gtcatccgtc
     7081 caaaggtttg gatgcggcgc tgggcgtatt gaggattttg cgtgcgcagg atgcccgttt
     7141 tgaattgcac atctacgggg gcaaccgtct ctggggtggg gctgagcagg ctccggcgca
     7201 ggaagacggc gtcttctatc atggcatggt cggtcaacgt gagttgtcac gtgaactgcg
     7261 taccagtcac attgctttga atcttcaaga ccgcctcgaa ccctttggcc tttccttgat
     7321 cgaagcaatg gcttcgggca atatcgtggt ggccagcccg gtgggggcgt atccggagat
     7381 tgtccgcgat gggtatgatg gttttctcgt ggaagggaat cacatggtgg aggaaacctg
     7441 gcaaaaggtg gcgcaaatgg cgttgacttt ggtgagtgcg cccgattttt cgagcaccat
     7501 ccgacacaat gcgcaagccg ccgtgcggaa ttgggaaacg gtggcacgca cctgggtcgg
     7561 tcattgggaa tgggccatgc atgtctctga gcggcggatg tgggcggatc cctgcccgga
     7621 ctgtggcggg ctgcaactgc ctctggcaga cggttatcat tgcgcctcat gtggtttgta
     7681 tctcaggaaa ggctgaaaac tttaatgctg tataaactct atcgaaaagc acttaatctt
     7741 tatcaacaga atcccgaatc gctgctcgca cgccttgtgc ggatggagat caactggctg
     7801 gtgaagactc tgcaaaggcg ccagcaattt cattttcccg cacgggcaac tggcgggtgg
     7861 tggtggatcg aacgtttccg ctttgagatt ctgatgcgct ggctggaagt cgaatcggtg
     7921 gaacgtgttc ggaaggtggt tcagcctggc atgaccgtgg tggatatcgg cgcgcacatc
     7981 ggctattaca cccgcctgct tgcagacctg gtcggcccga gcgggcgtgt gctggcgttt
     8041 gagccgaacc ctgagaattt cagtgtgctg caaaagaata tgtctttgcc gcgttaccgc
     8101 catgtggaac tgtttaacta tgcggttggc gaacgggaga gcatcctgcc tttgtatgtt
     8161 tcacccggta acagcaatca cagtttgatc gaaggctata ccaaggcgga aggcgtgatt
     8221 gaagtacgct gtgtggacct tgattccttt ttgcaggaac gcggcatcga acagatcagt
     8281 tttgtcaaaa gcgatactga aggcgccgag cctttggtgt tgaaaggcat gaagaacatc
     8341 gcggcgcgtt ctacaaacct gcacctgctc atggagttga atcccactgc cctgcgctgt
     8401 ggacatgtgg agcctgagca gttcctcgaa agtattcgca gcatggggtt cgagatcgaa
     8461 atgatcacct cggagctttc agccaaagag agcgacgcca atatgttgtg tagcaaggtg
     8521 ttgaaaaaat agacctcttg caaaattctg aaatcttaac caccgaggca ctgaaacact
     8581 gagaaaatca aataaagact ccgtgtcttt gtgcctcagt ggttcaaatg gtttttttat
     8641 ccagtaaaga aaacgccgcg atggtaaaaa tctgcatttt cagcatgaat tagaattctg
     8701 atcttgaagg tgtaaagtag ccaatctggc gacaaagaat tttggagtgc aaatgattca
     8761 gcttttcaaa aaaacatcca tcatcctgct tgtgatcctg atcgtgctgg cggcccaacc
     8821 gtccattgtg gcacatgccg caccgcctgc ccccattgag atcaccctgt accagcccga
     8881 tggattcgcg tttcaggcgc gggcctgggg cgatgaatgg tcgaatggca tggaaactct
     8941 ggatgggtac accatccttc aggatgggca gagtcttttc tgggtgtatg ctgctgagcc
     9001 aactcatgac agcctgcgtc cttcaacttt gatcgtcggc agggatgacc ccgccgccgc
     9061 aggcctgcct ttgcatcttc gcccgaatgt gaatcgtcct gcgggcagtc cgcctgcagg
     9121 gtttcaaccc attccagaag acagcatcaa cgtggggaca cagcgggtgc tggtgctttt
     9181 ggcgcagttc cagaatcaac cgtcgctggg caccgatgcc gcttattgga atgaccggat
     9241 cttcggtccc gctttgagcg tgaaaaaata ttacaaggaa gtttcctacg gacagctcaa
     9301 tctgcttcct gccattgaaa actacggcac atccagagat ggcattgtcg gctggatcac
     9361 actgccatac aaccatccca actccggtgg aaacgtttcc gatcacaatc gccagatcac
     9421 acgcgatgcc attttggcct cagccccata cgtggattac accgtctacg acacgaacaa
     9481 cgacggatac atttcgaaca aggaattaca catcattgtg gttgtggctg ggtatgaaac
     9541 agcctttgcc agcaccagtt cctgcaagcc cggcatttgg gcgcattact ggggcttcga
     9601 aggcggcaag gtccctgcgc cggtggtgta cggaaaaacc attggcgaga tcagcgcggg
     9661 cggtggatat gtgcaagtgg gcgagtggca ttgcgccgac ctgcccgggc ttacgcccgg
     9721 ttaccccgcc acgatgggcg tcatcgcgca tgagttcggc cacagtttga atctgcccga
     9781 cctgtacgat accggcggcg gttcatcggg catcggcgtt tggggcatca tgggcatggg
     9841 tacctggaat acgactggaa ctcagttggg tgacctgcct gcacaccacg atgcctggag
     9901 caaatggtac gaaggctgga tcaccccgct tcagcttcgc ggactttcag ctggtcagcc
     9961 gattccccag gcagaatcca acccgacagt tttccagctt ttggataacc cgaatggcct
    10021 ggattggaaa ttcaaatcaa actcaggcac aggcgaatac ttcctcgtgg agaatcgtca
    10081 gcagaccggt ttcgatgcag gcctgcccgg ttgcggcctg ctgatctggc acattgacga
    10141 aagtgtgccg ttcgacaact cagccaatgc agggaatcca cgcaggctgg tgtacctcgt
    10201 gcaagccgac ggcaccgatg aacttggccg gggccgcgac accggcgatg caggcgaccc
    10261 cttcccgggg tcacgatggc ggcaggcctt ctccgcttcc acaaccccga acagcaattt
    10321 atatgatggc agtccgagca gcgtcaatgt gttgaacatc agcggctgtg ccagcaccat
    10381 gaccgccgat ttcctcacac ccggtgatgg cacttttgca gatatcgctc ccgattattg
    10441 ggccgtggat tcgatcgaga agctgtatca aaacggcatc actcagggat gcggagtcta
    10501 tccgctgatc tattgccccg aaagctcaac cacccgcgct caaatggcga tcttcctgtt
    10561 gcgcagcaaa catggaagta gctacactcc gcccgcggcc acaggcacgg ctttcgccga
    10621 tgtccctgct gaccattggg ccgccgcatg gatcgagcaa ctggtcactg agggaatcac
    10681 ggcaggctgt ggtgacggaa attactgtcc tgaatccgag gtgacgcgcg ctcaaatggc
    10741 ggtcttcctt ttgcgcagca aatacggcgg cagttacact ccgcccgcgg ccacaggcac
    10801 tgccttcaca gatgtccctg ctgattactg ggccgccgca tggatcgagc aactggtcgc
    10861 cgagggaatc actgcaggct gtggcgatgg caactactgt cccgaatccg ctgtgacccg
    10921 cgcacagatg gcggtctttt tggtgaaaac ctttgctttg ccgtagggtt gccgggaatt
    10981 tgaatgagtc tacacaaagg cgtgttatga caaaaccatc aggtaaatgc ccaaaatgcg
    11041 atggcgacat ggtcagagga tttattctcg attattcaaa tggcgcgatc gtcctcccaa
    11101 actggcatga aggcgtgccc caaaaatcct tttggctggg aacttcagag ccaaaaaccg
    11161 agggaatccc tgttgccgca tttcggtgtt cgagctgcgg gttcgtggaa ttttattctc
    11221 acaaaaagta caaggcaaaa taaggacgtt ctcgccgccg tcctcggcgg cgagaacgta
    11281 gaatggattg ctgaggaaga gttggatact taccaagatt tgaaaatcac cgataagctc
    11341 atggattatt tgggaattgc tgatattgac taccttagtg cgcgtctatt atttatgtca
    11401 ggttttccaa tagtagcaat ggctaaagct gccgaagcgc ttgagaagtt atttaaaata
    11461 ttgattgtga tgatcacacg gatttattca caagtagaac tgacagatga tcaactcaag
    11521 aaagattatg ggcatgattt ggggaagctc ttgaagggtt acaacaaaat tgcacccgag
    11581 tcggtgaggt tgattgggga tacaaccatc ttggataatt tacggaaggc ttataatttg
    11641 agatatacgg ggagagacaa agagattaat ttagaattct ctttggaaga atttgacaag
    11701 tggtatgtgt tattgcgtaa tcttattgtt cgaaacaccc caaaagaact ggcaatagaa
    11761 gtaagacaat ttggtacatt tcacggcaag ctgtatgaag caggtatgga aaagatactg
    11821 tctcagtata gaactttgcg accaggtgat attcttcggt atcaaaatgc aatgtttgac
    11881 aaacttgatg tcgaccttca atgtcctttg tgtcagtatc ggagtggcga aattcgggaa
    11941 cctactttag atgacagaac atagcacaga atgtgccttt tatttttagt gtggtacgtt
    12001 ctgtacatta ttgtcttggt gacataaact ggctttgtag cgcgacactt atgtcgcgct
    12061 acttttttct ggcaatttat catcattgcc tcctgcatgg agttcgatga taataggcgg
    12121 acatttcctg gagaggcagt atagacagtg gataaaaaca ataaaaagac cattctggtg
    12181 gcagggtatt acgggtttgg caacgtcggt gacgaagcca tcctcagcgc catcctgacc
    12241 gacttgagaa aacaacgcga gggacttgaa ttcatcgtca tctcgtcgaa ccccgaagaa
    12301 accgccgcat cgcacaatgt ccgttcggtg cattggaagg agatcaacgc cttgatggat
    12361 gcggcccgcg aatgcgacct catcatcctt ggcggcggcg gcatttttca ggattattgg
    12421 ggcacccctg caggcacgca tttaaccgaa tttcactggg gcatttcgta ttacagcagc
    12481 atcggcttgc tggcttccct gttcgataaa cccttcatga tctattccgt gggcattggt
    12541 ccgctgttga ccgatgaagg caaacgctgg acgcgcctcg cctttgaact ggccgatgcc
    12601 gccacggtac gcgatgtcga atcaagagac ctgctggctt cactgggttt tcccaaaaaa
    12661 caaattctca tcaaacccga ccctgcgctc aacctttccc cgaaactgaa agccgcggcg
    12721 gagattcttc aagctgaggg catttctccc aaagaacgcc ccatggtcgg cgtttgtatc
    12781 cgcaattgga ccgaaggcga aaaaggcggc gcatggaagc aggaactggc gtccgcgctg
    12841 gatgccttcc tcgaaagaca taatgcgcag attgtgttca ttcccttcca ggtgggtgag
    12901 cacgaactcg agaatgacta cactgtttca gtggaggtca tctccatgat gcagaatcag
    12961 gaacggacga aggttttacc gcaagcctat tccccggaag tggtcgaagg gcttatcgcc
    13021 cgctgccgcg ttgtcatcgg catgcgtctg cattcgcttg tttttgcggc cagtgtcggc
    13081 gttccatcgg ttgcattgat ctacgatccg aaggtcagga acttgatgaa gttcctcggt
    13141 ctttcagagt acgcgctcga tcttcagacc atgaatgtgg attcgctttc agataccttc
    13201 cactaccttt ggatggacca aaaacaggtg cgcgcatcac tggcggagca gggtcagaag
    13261 tttaagcagc ttgttcaaac caacaccact ctggctttga aattgctcga ccaaaagacc
    13321 aagcgtattg aaaagcccgt ggcgttgcaa ttgatccgcg atctggctgt taaacagacg
    13381 ggcgccttct tcgtccgtga aaaagaggcg caggctttga ctgccgaagt agcgagcctt
    13441 aaccagcaag tggccgacca acttgcacgc atcgccatca acgaatacga gatcgaccgc
    13501 atccaaaaga tcgtggatta tcaacagtat caactgcatg aaatggtcca cagcacttca
    13561 tggaagatcg cgttgatttt ccaaaagatc agaaccaccc tcattccgct tggcagccga
    13621 cgcgagaaac tcgtgcgctt tgttttccat tggttcgttc gcaaaccatt gtctttgatc
    13681 tcgcggatac ggacctcgat ccgagtcaac ggggtgtttg gtgccatcct taagggcttt
    13741 gtcgccattt gggttgcgct gacctacccg atcaagaagg ccatcttcaa gcaacgctat
    13801 gaacgcgagt tgaaagagtt ggaagccatc atcgccggac ataacggttt cttcgacctg
    13861 ttccatattc cgatgggttg gaacacgatg ttattccagc gcttccagca tgtttcgctg
    13921 caatctgcca aattgggcgg aattgccatc tatggcggcc accccactgt ggacaaggat
    13981 atctttgttt ataaaaaggc tgccgagaat ctttacgttt tcaaagccac cgatccgcag
    14041 atcacggaac gcattttgca agcccttgag aaaaaaggca attccaccgt gctacgcatc
    14101 gaatccattg acctggtgac gaccgccgaa gatgtggcaa acttcctgcg ccgcggtttc
    14161 cgtgtggtgt atgaatacat tgacgagatc acatcagaca tcaccggcaa cgtgcccgaa
    14221 cttgtccgtg atcggcatca agccttgttg agaaatgaag ccgtgtttgt tgtggcaacc
    14281 tccgatcaac tgtatgaaga agtagcccgt caccgcgcaa agaattttgt cctcagcacc
    14341 aacggcgtgg acctcgaaaa ctggcgcatt cccaaaggcg agccgcccgt ggatatgaaa
    14401 tccgcgttta ctggaaatac catcgttgcc tatcatggcg cgcttgccaa atggctcgat
    14461 tacgacctgc tgcgcatgat cgccgatgaa ggcagttatg aactggtgct gatcggccat
    14521 gagcatgacg atgccttcga gaaaagcggt ttgaaggggc atccgcgggt gcatttcctc
    14581 ggaagcaaac cctactcaca gttaaatcaa tatgcgatcc attacggtgt gacgatcctg
    14641 ccgttcaaga aatccaatct caccgaggcg gtctcgcctg tgaagatatt cgaatacatg
    14701 gcagccttga aaccgatcgt caccaccgac ctgcgcgagt gcaagaaata caagtcctgc
    14761 cttacggctg aaacgaatga ggaattcatg cagcaattga aacgtgcctc tgaactgcgg
    14821 agcgatgaag aatatctcaa ggtgctcgat acggaagcga acgagaattc gtgggaacac
    14881 aagaccatcg agatcctgca catgaccggg gtgaaactgt gatcaatcga atacttcagt
    14941 tcgcgctgcg tatttatcgc aaattgccaa tgcctgagcc gcttcgccgc aggctttcga
    15001 tgctgtcaca gcggatcttc cgtccgcgca gtgcgcctgc catgcccgag cctgcgaaga
    15061 cgaaaatgta cgaacagtac accaaacagg ttcttgcaat tcccgataag accgatgact
    15121 atgtggatct ggcttccgat gcctatgtgc cgcaggcggg cgatccgctt ttgcttgcat
    15181 attacctgcc gcagtttcac ccaacccctg aaaatgacga gtggtgggga cgcggcatca
    15241 ccgagtggca caacgtggcc aaggctgtgc cgcagtacgt ggggcattac cagccgcgcc
    15301 tgcctggtga gttgggatcg tatgacctgc gtctcaagga aaacatcgcc cgccagatcg
    15361 aattggcgaa atcgcacggc attcacggct tcgcctatta cttttactgg ttcgatggcc
    15421 gtcgtttgct tgataaacct cttgatatgt ttttgaataa ccccgatctc gatttcccct
    15481 tctgcctgtg ctgggcaaac gaatcgtgga cccgccgttt cgacgggtcc agcggcgagg
    15541 tcatcatgta tcagaatgaa tcggtggaga gttataaatc cttcatcgaa agcatcaaac
    15601 cctatgccgc cgacaagcgt tacatccgtg tggatggccg cccggtgctg atcgtgtacc
    15661 gcccatcttt cgtgcctgat tgttccaatg tacttgcgca ctggcgggca tattgcgaaa
    15721 aggaaggact tggcaaccca tacatcatcg cggtcaagga aaacaccttt gatggcgacc
    15781 tgttggcgct tggttttgac gcccaaagcg aattccaccc gggcacggtc ttccgctatt
    15841 gtaaaaacat caccaacgaa atcaacttca tgcgcgatga tttcggcgga atcgtcctcg
    15901 attacgtgga cctggtgaag aacaagcaat atttcaagtt tcaggcgaag aaattacacc
    15961 gtgccattat gcccatgtgg gataacacgc cccgccgcaa taacaccccc atgatcttcg
    16021 aaggcgccag ccccgccttg tacaaggaat ggctcattga cattctgcgt gaagcccgca
    16081 gcagcaagga acttgatgcg cccttcatct tcgtcaatgc ctggaatgaa tggggcgaat
    16141 gtgcctacct cgaacccgac cgcaaatacg gttacgcctt cctgcgtgcc acccgcgaag
    16201 ccattgaaga gacccgcaaa gccgaaggcg aatagatatc ccttcgcgtc tctttgtaat
    16261 tcttcgtggc ttatttcaac atgaatgccc ccctcatctc catcgtgctt gttgcctgga
    16321 acagcgccgc ttatatgccg cgctgtttcc agcatctgct tgcgcaaacc gtgcaggatt
    16381 ttgagatcat tgttgtggac aatggctcaa aggatgatgg gtgtgtgggc ctgcaggaaa
    16441 aatatcccac ccttgccctg cgcgtggaac ggctcgaaac aaatcacggc ttttcatttg
    16501 caaacaacct tggcgcacag cttgccactg gcaaatggct gaccctgctc aacacagatg
    16561 ccttccccga acccgattgg ctggagcgtt tgctcgaagc ggcccagatg cacccgaacg
    16621 catttttttc ctcgcgccaa cttcaggcca atacacccga attgctggat ggtgaaggtg
    16681 acgtgtatca cgtcagcggg ttggcgtgga gaaacggtta tggctcacgc acctatcccc
    16741 tggatgaaac ccgcgaagtc ttcagcgcct gcggagccgc cgcatttttt ccgcgcgaaa
    16801 aatttctcag cgtgggcggt ttcgacgaag attacctttc ctatcacgaa gatgttgacc
    16861 tcggttttcg tctgagatta cacggcttga agtgttttgt agttccccgc gcggttgtgc
    16921 atcatgtcgg ctcggtcacc ttcggcaaga aaagcgactt ttccatttat cacggccacc
    16981 gtaacctggt gtggacctac gccaaagaca tgccgtctgt gctgttttgg ttctacctgc
    17041 cgctgcatat tttcatcaat gtgttcttct tgttttactt caccgccaaa gggcagggcc
    17101 gtaccatctg gcgcgcaaaa ttcgatgccc tgcgcggatt agcccccatg ctcaaaaaac
    17161 gcggcgcgat ccagaaaaat agaaaagcat ccatcgctga attgcatcgt gccatgtcgc
    17221 gcaattggct ggctcccttc ctcacttttc taaaacgcaa cacggaaaat tcatgaaacc
    17281 tgatatttcg atctgcatta cgaattacaa cgcgaaggat ttcctgcgcg actgtctgcg
    17341 gtccatctac gggacaacgg atacgctcac ctttgagatc atcgtcgttg acaatttctc
    17401 cggcgatggc ggccctgaaa tgatccgcag tgaatttccc catgtgaaat tgctgaccaa
    17461 cgaaggcaac accggcttta cccgtcccta caaccaggcc atgcggctgg cacaaggtcg
    17521 ccatgttgtc gtcctcaacc ccgatactgt catctttccg aaagcctttg ccgaacttgt
    17581 tggcttcctg gattcacatc ccgaggtggg cattgtcagc cccaaagtgt tgaaccgtga
    17641 cggcacgctt caaaagcaat gccgccgcag tgaagctcgt ccatgggatg cgatctgtta
    17701 tttctcgggt ctttcccgtc tttttcccca cgacaaacgc tttgcaggat atctgatgac
    17761 ctacctgcct gaggacctca ctcacgaagc agaggcagtt tcaggttcgt gcatggtcgt
    17821 tcgtcgtgaa gtcgtggagc aggtcggata tttcgacgaa gatttcttcg cctaccagga
    17881 ggattccgat tattgtcgcc gcacccgtct ggcaggttgg aagatttttt atgtgcccac
    17941 ggcgcagatc acgcactacg gaggggaggg cggctcaaag gtccagccat accgcaccat
    18001 catcgaatgg catcgttcgt attatcttta tttccgcaag cacttcgcga aggattactt
    18061 cttcctgttc aactatttgt attatttgat gatgctgacc aagctcggct acgccctgtt
    18121 cacaaacctg ttccgcagaa agaagatcgt cagcacaccc aaaccgtaaa tcaaaaactg
    18181 tccactccac ggagcggaca gtttttctta acgtgcttta aaagccacag gaaaattcag
    18241 gggtcaattg ttcttttgcg ctggcaattt gttgccttcg ccttccttcg taacttccac
    18301 ccaaagcgat gccgccaatt cataaccgac gacatggtcg tgcggcttca tgttcaatcg
    18361 caacggaccc ttgaacggcg cacaacttaa caagtgaaac gacgtgccgg gcacaagacc
    18421 caactcaccg atgtaggtca gtttttcgaa atcatgggtc cgcacacggc tgatgacaca
    18481 atctgaccct gagggcacat cgatcagtga catgtcccgg accacaggca ttaccccatc
    18541 ttttgtggga atcggctctc cgtgcgggca gcgtgtaggg cggccagcca gttcatccat
    18601 acggtcttcg atcatgtcat tgacgccgag ttcgaaagta tcagccagtt gatgggcagt
    18661 ggcccaatcg tatttcatca ccttgacgag gaagacctcc gttaggcgat ggcggcgcag
    18721 ggaaggcatg gcgatctttt cgccagtggg ggtcaggcgt acaccgcgat acggctcatg
    18781 gacaagatac ccgttttttt gcaaacgttt gaccatgctg gcaatggcct gtgctgatga
    18841 ttcaacatgc gtgcttaaaa gcgaaagcgg cacctgctca tgatcttgtt gcaggcgata
    18901 gatctccgcg gcatagcgtt gcatagcaga actggctgac attccttgta aacaactcca
    18961 aaatcttcaa ttcgattgaa aatccaaatc aactctattt agataaaatt taatacaaat
    19021 atctagatta ttcaaatccc cgaaattata ctacgcgtcg cttgaacagg atgaaagtaa
    19081 tccaatttat ttatcaaagg atgcagtatg tttcaaacca ttcaacccct caacctggat
    19141 ctgaccgttg tcgaacgcca gaaaaccatc cgcgatgctt ataacactga ccgttcgcag
    19201 atgacattaa tgctggccca tcaactccga atccctgaag tggaaattgt tcgcgcattg
    19261 gagggtgact ctgcacatga actggatttc tcacgctggc aggagatcgt ctgcgctttt
    19321 gaaacgctgg gcaatgtgca tgtcatcgtc tcaaatggag ccgcaaccat cgaagcctat
    19381 ggacagtttg gtaatctcac aaatagcgac ggctttttga acgtccgcac caaatcgctg
    19441 gacatgcaca tccgcagttg ggaattggat tcggtctttg ccttccgcaa gcccagccat
    19501 cttgatggga tcgagacgct cagctttcaa ttcttcgatc ggcaaggtgc cgccgcattc
    19561 aaagtctttc tcaattttag cggcaaagtc tctgcgcccg cactggtgga aagatacgat
    19621 gcgctgatca ggcaattcgg gaaaaagtaa gccagctaat gcatgcccca aaccctcact
    19681 aaactctagt aaaattctac ataaatctac atggctctat gaagatttac gcctaaaata
    19741 cacaatgagg tgagggcgcg taaaacgccc ttgctttgtt taacaaaacc aatgaccaag
    19801 agacaaaacc acccactatg ttcatcaaac gacttcaatt agccctgatc cataccgccg
    19861 ttgccatcac ccttgtgccg atcaacagca cactcaaccg tgtgatgatc ttcgatctgg
    19921 gcgtttccaa aaccctgttc acacttttgg ccattttccc ttatttactt tcgcccattc
    19981 aggtggccat cggctcgttc tcagaccgac accccatcat gggatatcgc cgtactccat
    20041 acatccttgc ggggttactc ctttgcgtgt tgggtgtggc cattgctccg cagatcgccg
    20101 tgttggtcag tgaaaacttc acacttggga tcatggtggg catcttcgca tttgggttgt
    20161 ggggcatggg ctacaacctc tctgctgttt cctacctttc gctcgcctcc gaactttcag
    20221 gagaaaaggg gcgtggaaaa accgtcgcaa cgatgttcac catcatggtg atcggtttaa
    20281 ttgcgacggg gatcagcctg agccgcatgg tgacagttta tgaccctgaa acgctttcgc
    20341 gtgcctttct catcgtcgcc gcttctgccc tgacccttgg cctgctcggc ctcttcaagc
    20401 ttgagccgcc ctttggcaga tcagaccaaa ccccacgctc tgaaacatac accgtcaaag
    20461 agatgaccgc tgccatcacc tccaaccctg tggcaaagat attttttgcc tatctgttgc
    20521 ttttgcttgc cgccatcctc ggtcaggatg ttctgctcga gccctttggg gcgcaggcat
    20581 tcggcatgag cctcgaacaa acctcacgca tcgtatccat cagcagcact tttacactgg
    20641 tggcattcat tgtggctggt gttctggaag ggcgggtgaa gaagaaatac attgcccaaa
    20701 tgggcaacct tggcgcgctc atcggttttc tcatcatcgt cgtcagcggg ttgacaggca
    20761 gcctgacggc tttttacctt gggatcaccc tgctgggatt cggcacgggt atttccacag
    20821 ttgcgaacct gtcgttgatg ttcgacctga ccgtccctga aaaggttggg ttgtatattg
    20881 gcgcatgggg tttttcgaac gggctttccc gcctggttgg gttgttgatg gcgggtgtgg
    20941 ttgcagacct ggccacacag atgacaggca gtgccctgag cggatacctc gttgtcttca
    21001 gccttgaagc attgatgctg ctggctgccg cggtcatgtt gtatcgtatt gatgtgggtg
    21061 cattccaaaa gcaggcccat gaaccgtcat atgtggaaaa agtggcgctg agcgtggagt
    21121 aattatgacc gacgaatggc tttccctgag tgatgcggca aagatcctgg gggtgcatcc
    21181 tggcacggtc aggttatggt ctgataaagg ttccctgcct acacataaaa cacaaggcgg
    21241 gcaccgtcgt tatcggcgca gtgaaatttc cctttgggca gaatcaaatc acaggtcgaa
    21301 tgcgatcgaa cccgaaggga tgatgaatga agtcgtcaaa aatgtccgcc tgcagatttc
    21361 agagggacgc ttgcaggctg aatcctggta tcaaaaactg gatgacgatg cgcggacgca
    21421 gtaccgcatg agtgcaaggt cgctgttcca tggcttgatg aattatgtcg caacccacgg
    21481 ggcagaggct gaaagcgaat cttatgccat cggttatgaa tatgcttcac gagcccatcg
    21541 ttacagtttg aattacgtgg atgcagccaa ggcttttttg tttttcagaa atacgctgat
    21601 cgaatcagtg atcaaggtgt acggcgaagc caatgtgcct tcgggacaaa ctgcagaaat
    21661 gttccaaaag atgcatgtgt ttacagatga aattttgatc agccttttgc aaacttatca
    21721 gtcattgaat gccagccgtt gaacggttgg catttttatt tgttcaccca tcgttgcgga
    21781 acggcgcagc ccttcgcgat gatataaaac ctaaaggagt ttcaaatgca agctgttacc
    21841 cactatattc ccatctttac caccgtgatc tcattctctt tcacggttgc tttgtataaa
    21901 cactggcagc gcaagccgga agccagatac ctcatgtggt ggaccattgg agcgttttgt
    21961 tttggcatcg gcacgttgac cgagtccatt aatacgctgg ttggttggag tgtatggaac
    22021 atgaaagcct ggtatatcag cggcgcgttg ctcggtgcat tcccgcttgc gcaggggacg
    22081 gtgtatctgt tgttgaagaa aaagaccgca gatttcctgg cctggttctt cgtggtttat
    22141 attgcagtcg cttcggtggc gatctttctc gccccggtgg atatgtctct ggttgaccca
    22201 gcccgcctga ccggcaaagt aatgacctgg acctgggctc gtatgttctc cattcttccc
    22261 aatacttatg ccatgatctt tcttgtgggg ggcgcggcct ggtcggcaat tcagtatgcc
    22321 cgcaaattgg attctggcag ccgcgtgctt ggcaactggc tcattgccat tggcgcactt
    22381 ttgccgggca tcggcggttc cttcacccgt tttgggtacg tggaggtgct cttcgtgacc
    22441 gaattcatcg ggatctcttt gatttgggcg gggtatcgtg ccatgaccca gacgcggacc
    22501 gtatccattc acaccgcgca ggccgcagcg gattagagaa gcgggtttgc atccgcaggt
    22561 gacgattttt gaggggcgtg tcatctgcgg gtgcatttgt ctaaaaactg catcattgct
    22621 tgcatcatca tattgatgtg gttatacttg ggcttatgcc tatcccatcg aaaatcggac
    22681 gatatgaggt caaatcagag ttggggcgcg gtggcatggc tactgtgtac cgcgcatatg
    22741 atcccagctt cgaccgcgaa gtcgcaatca aagtcctgcc acgcgaaatg ttgcacgatc
    22801 cacagtttcg tgggcgtttt gatcgggagt tgaagatcgt tgccggtttg gagcatccct
    22861 ccattgtgcc ggtgtacgat gtgggcgagg aagacggcca gccttacttc gtcatgcgtt
    22921 acatgacggg cggctcgctt tcagaccaga tcgcattggg gaagatctct gtacaagata
    22981 cggcccgcat tgttgaacgg attgcaatgg ggcttcacta tgcccataaa aagggcattg
    23041 tccatcgcga cctgaaaccc gataacatcc tcttcgataa caacggtgac ccgttcattt
    23101 ctgatttcgg aattgcaaag ttgaccgaat cttccagcgg cagcctgaca ggcagtggcg
    23161 tgatcggtac acccgcttac atgagtccgg aacaggcaaa aggggagccg atcgatgggc
    23221 gcagcgatat ttacgggctg ggcgtcatca tttatcaaat gttgagcggt caacagccat
    23281 acaacgccga tactcccatg ggtgtggtgg taaaacatat caccgatccc gtgcctgaaa
    23341 tcctcaatat gattcccagc ctgcccattg aagtggatgg catcatcaag atgtgcatgg
    23401 ccaaagacaa gacccgccgt tacgccaatg ctgtggaact ggccaaggcc atgaatatgg
    23461 ttgcatttgg aaatgaaggc aaggctggtc tttcgaacac cgggtttcga accgttcttc
    23521 cgggtacatc caccctgcaa tcgccccgcg cgcggacagg ccttgccgtg gctggtgtgg
    23581 ttttgctggt ggttgtggtt gggggattcc tgttgcgcaa tcaattattc acccagccgg
    23641 aagccacttc cacacctaca ttgaccgccg ttgttttcac ttccacggtg gaagaagttc
    23701 ccaccgaaaa accgaccctg acctccgagc cgccgaccga aacggtcgct tcgcctctgc
    23761 cttttgctcc agcctgtgcg gaggggatta ccatccccgt tcccgaagtg cgcgagacga
    23821 acaaggcatg cgtcaccaag attccctaca cctcggtttc catcccagag ggtgcggaat
    23881 tcgaatcact cagccccgat atgacctgtg ccatcgaaac cacatccgat ggaaaaagca
    23941 tcgtcgcatg tacgggcagg cagctttatt cctacgaact caaagtttgt acgccgcctg
    24001 tggtcgcgaa tgaagacctc aacaaatgtg aatcgggttc gctctttgag gcagccaatc
    24061 aatgttgtgt gcccaccccg aaagatggcg cgggctgcac cacctttaag atcgaccttc
    24121 gctcctgtca gtaaaatagt cccccgatat aaaaactcgc cgacaagcat agatgaacgc
    24181 ggattcaaaa agaatccgcg ttttgattca ccctcatatc tgatttacct gcggcaaaac
    24241 gaattaccgg aacagaatga taaaataaac aaatgggcac cctttttctt gtagcaaccc
    24301 cgatcggaaa cctcgaagat atttccccgc gcgccctgcg catcctcaaa gaagtccaac
    24361 tcatcgcagc ggaagatacc cgtcacagcg gtgtgttgct caaacacttc gggatcgaaa
    24421 ccaggctcac cagctatttt gaacacaaca aaattcataa attggatcag atcctctccg
    24481 cccttgcaga aggggatgtg gccttgatct ccgacgcagg gactcccgcc atcaatgacc
    24541 ccggctacga actggtacgg gctgccctcg cttcaaactt cgacgttgtg cctgttcctg
    24601 gtccctcggc ccccatcgcc gctttgaccg tttccggcct gcccacagac tcatttttat
    24661 accttggcta tcttccccat aaatcgagtg aacggcttgg ttttgtgggg caagttagta
    24721 acctgcccta cacgttgatc ttcctcgaaa ccccacaccg cttgcttgac tcacttgaag
    24781 acattctcgc cgtcctcggc gatcgccgca tctgcattgc ccgtgaaatg accaagcgct
    24841 acgaagaata ctggcgtgga gatatcagtg gcgcgctgga atatttcaaa tccaaagacc
    24901 ctcggggcga atttacgttg gtggtcgaag ggaagaagaa ggaagaaagc agcaagtggg
    24961 aagaatctga gttgatggat gccattcgga aagaattgca aacgggaagg tccgcaaagg
    25021 aaatttctgc agacctggcc gggcaaagcg gttggaataa aaaagacgtg tatgcgttgg
    25081 tcaatttggt gaaatgaacg aacagaacag ccccaccatc ccaccactgc ttggctcctt
    25141 ttctcaacgg ttgctgtatt ggatctttat cccagttgct ccaatagtca acttttcgtt
    25201 tgtttcggcc atgctgcctt tatggcagac agggaagttt tccgattaca tgctgttgat
    25261 gctttccccc gaagcagccg cattgaccct gcccctgctt gggtattcgg ttatctccta
    25321 cctgatgctg atcagtgacg aagaacgcca gagcgaatcc ctcgttattc gatttggcat
    25381 ttatggcgga gtgctccttt cctttcacta tagcctcctc acagccctgt ccatggactt
    25441 gtcgcctgtt ttattcggct ttgttttttt tatatttctc ccgcttctta ttccaaaact
    25501 tcgtgcatgg cttcagaacg aaaaggtttt tcgtgcattg atttttgccg cgtttgcgat
    25561 tctggttttg tggcacattg tcgctgcaat ttttgattac agcacagccc ctgtccttct
    25621 tctggtcatc ttggtgccga tgtctgcacc cttttggtgt ttcttcatcg ctttgcaagc
    25681 cgcccgttgg ttatggaaat atcatgaacc caaactcacc catgcgcacg gttttgggct
    25741 ttttacctgg ctttctgcgt atggggttgc cctgtactac aacattacga agatgtacga
    25801 actatatgcc accctgccga cccagccgcc caattgttac attgccaccg ccgccgcaaa
    25861 tggacattct gcttttgtcg gttcacaaga gatccgcctg gagaatggaa tatccatgcg
    25921 cgtgaacagg caattgcaaa atctgaaatg tgccgaactc accctgatgg ttatcgcgcc
    25981 gcatctccac aaaattctcc gctccgttta tgatcttttc ggaaaggcgc ttgcccgaag
    26041 aattcagaat cccctccttg cggatgccgc ctacctttct ctgaaaccat ttgaatggac
    26101 ggctgtagcc gtattggagc ttatcgtccc gaattgggat gaccttgcct ccaaaatata
    26161 catccgctag ttgtagattt cagcagtact ttattgcgct gctgttgatt gaagccaccc
    26221 tgtttatatg tgctcgtggt gaaaattctc atataggata aaaaatggaa taccttaccg
    26281 ctatcttctc cagcattctc ccccagcttc cgtccctgtt ccttcacgcc gtcgggcttg
    26341 gattctccat ttcacgacgg aaggaatatc cgcgcacggc gcttgccgcg ggcatttact
    26401 tcgggctggc tcttttgatc ggactggcca cgcaagtgta tgttgttttg cccatgtaca
    26461 tggtggatca gggcggttct acacaggata tcggcagcat cttcaccacg ttgaccttta
    26521 tctgtgttcc gtttcgagtt gtaatggaag tcgcccttct ttatgctata tttgctccac
    26581 gcaacagtaa ttccagtgac cccaatcaac ctattttcat gggagataaa aatggctgaa
    26641 aagaaacctg aagaaaagaa agatgaaaag aaggacgaga aacccgctcc aaaagacaac
    26701 ctggtggaaa ccaaacactc ggtaaaaatc ggcgggaaga cgatcaaata caccgtcacc
    26761 acgggaacga tggtcgtcaa agaagaagtg tgggataagg acaaagaggc cgaggtggat
    26821 aagccccgcg cccaaatgtt cttcgtggcc tacacactcg atggcgtcaa agacagatcc
    26881 aaacgcccga tcaccttttc cttcaacggc ggaccaggct cctcgtctgt gtggctgcac
    26941 atgggtgtgt tgggaccgcg tcgcgtcgtc ctcaccgacg aaggcgaaat gcccccgccg
    27001 ccattcaaac tcaccgataa tgctttttcc atcctcgatg aaaccgacct cgttttcatt
    27061 gacccgatga gcactggcta cagccgccct gttgaaggtg agaaatcacg cgagtggcac
    27121 accttctcga aagatattgc ctcggtcggt gatttcatcc gactgtattc cacgcgttac
    27181 aaccgctggc tctcaccgaa atttctcgca ggcgaatctt atggcaccac ccgcgcctcg
    27241 ggtctttcag gctatttgca ggaacgtcat ggcatgtttc tcaatggcct catcctcatt
    27301 tctgcagtgc ttgatttcac caccattgat ttcaacctca ataacgacct gccgtatatt
    27361 ttatttgtcc ccgcttatgc ggctacggca tggtatcacg gcaagttgag cttcaaagcg
    27421 tcgttgaagg aagtcgttcg cgaagctgaa gaatttgcaa aaggcgaata tgccaccgcg
    27481 ctgctcaaag gtgcggctct cactcctgaa gaacgcgcca gtgtcgtcga aaaattatcc
    27541 cgttacacag gcatttcaaa ggagtttatt gaacgcacca acctgcgcat ctacgatcag
    27601 catttcttca aggaactcct gcgcgagcgt gggaaaaccg tcggccgttt ggacagccgc
    27661 ttcacaggta tagaccgcaa tggcgtcacc gccatgcccg attatgaccc gctgttcgcc
    27721 caggtcaatg gcccttacac ggccacgctt aatgattacc tgcgcaccga gctcaaattc
    27781 gaaaccgacc tgccttacga gatcctcagc gacaaagtct tcctcaattg gtcgtatgag
    27841 tattttcaaa atcaatacgt caatgttgcc gaaaccctgc gcgcttcaat gacctttaat
    27901 aaatacctca aggtcttcgt cgccaacggc tattacgacc tcggcacacc ctactttgcc
    27961 accgaataca ccttcgatca tctcggcctc gacgaatccc tgcgcaagaa catcagcatg
    28021 gggtattacg aagcaggcca catgatgtac gtccacatcc cttcgctcaa gcagttgaag
    28081 aaagacttga gcaaattcat caaggacgcg agctaacgac cgcagacaga aggcggcaga
    28141 ccgatggcaa ttgaccgcgg tctgccgcct gatttcataa aacttttcca ccacctaatt
    28201 ccatgaacct agatgacctc gacctcttca aaaaaataga tacccgcaac atgctcgccc
    28261 aaattgacgg cctgccgaat caattgcaaa ccgcatggga actgggacaa accctgccgt
    28321 tgcctgcttt caaggatatt caaacgatcg tcatttctgc gatgggtgac tcagcggtcg
    28381 ctgccgaact ggtaattgcc tctgtttcct caagcattcg cctccccgtg gcattgcatc
    28441 gcggctacgg cctccccgcc ttcgccagcg gacctcagac tctcgttgtc tgcatttcgc
    28501 attcgggcaa ttccgaagaa gtgctcgacg ttttcgaagc cgcgcaaaaa aacaactgca
    28561 cagtgatggt catctccaca ggcggcgaga tcgccatgcg tgctgctgat aaaaatatac
    28621 ctgcctggaa attcgattcc aacggcgtag tggatacagc cgctgttgcc tacccttttg
    28681 gcctgttact cgccttgttc gcccgtcttg ggttgatccc tgaccctgcc agcgatgtcg
    28741 ctgaagccgt cgccatgatg aaacgctctc agcaacacat caccgccgat gtgatcgctg
    28801 caaagaatcc agccaaacgg tacgcgggtc aattggtggg gcgctgggtc accttcgtcg
    28861 gcacggaaaa tctcgccccc gtggcccgcc gctggaagat gcagatcaac caattggcca
    28921 aggctggcgc caattttgag atcatccctg aatccacgca caacactctc attgccacca
    28981 tcaacccgaa tcccacactg aacgctcaca ccatgaccct tttcatgaaa gcgcccagcg
    29041 accatccgcg caacaaactg cgttctgatt tgatgcgtca agccttcatg ctcgaagcct
    29101 tgaacaccga tgtgatcgaa gcccgcggcg actcggtcat tgctcattta tggaccttga
    29161 tgatctttgg tgactacatg gcgtattatc tcgccatggc atatggtgca gacccctctg
    29221 aagaagatgc gtttgtgaat ttcaaacggt cgctatcgta gtagagacac ggcgccgccg
    29281 tgtctctacc catcacgcaa actgttccaa attgaccgcc acaagcggaa gcgcttcgtc
    29341 cacgaatttt tcgaggacgg ggcgtttttt catgtggctg tagtgcgtgg caagtccgta
    29401 aattgcccaa gtggcgacgg tggcggggat ttcggtagga agttgtggtt tgttgagcca
    29461 gtgggataac aagtcaaaga tctgtttttt ggtggtggct tcagccagag actcgaattg
    29521 ttggctgggt tgggcacaac cactatttga cttaaccaga aattcgcaca cgacctggat
    29581 caggttgcgc aaattgtcag cggagtaggc acacatattc aggctgcgct tttcaatttc
    29641 agtccagaat gttttttgga tggcgtaatc cagcagggcg tatttatcgg agaagtgggc
    29701 gtagaaggtg acgcggttga tctcggcttt gttggcaacg tcctgcacgg agatggagtc
    29761 gaagcctttt tcggcgagga gagaggtaaa ggatgtcaga atcaggttgc gggtccgttt
    29821 gacgcgaggg tccagttttt cttcttcttt gagagtaggc gacattttcg ttcctttgta
    29881 gtttaagtaa acagtttcgg ttttttgtct gttgactctg tgggcgaggt tggtaaacta
    29941 agctacatat gtagtttatc ttacattcgt agtttatcaa aactcacaag gagagtcaag
    30001 aatgaatatc gcattatgga tcgtacaggg gttgctggct gtgttatatg ggatggcggg
    30061 tagcatgaaa actttcaggc ctgatttcta ccgcaataat ccgcagacgg aatcgtgggc
    30121 aaaggaacgg agcgattggg ggcttcgcca gatcggtatc cctgaatttc ttggcgcgct
    30181 cgggctgatt ttgccaatgg tcactggcat tctgccgtgg ctgacgccgc ttgccgctgt
    30241 tgggcttacg gtcattcaat tgcttgccct gttcgctgtg catttgccga agaaggaacc
    30301 gatcgtgccg aatattatat tgggtgcgct gtcggtgttt attgttgttg ggcgttgggg
    30361 acttttctcc taaggaaaca agggttaatg tagtctacaa taccagcaac tattacctca
    30421 catggagaac cgcgttatgc aaaatattga atttggattg gataccttcg gcgatgtgcc
    30481 gatggacgac tctggcaaac cggtctcgta tgcgagagcg atccgccagg tggttgatga
    30541 ggctgtgctg gcggactccg ttggcgtgga tgtgatcgcg ctgggcgaac atcatcgcgt
    30601 ggagtatt
//
LOCUS       DAOQLC010000044        30345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_481_length_30345_cov_24.746418, whole genome shotgun sequence.
ACCESSION   DAOQLC010000044 DAOQLC010000000
VERSION     DAOQLC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 30345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_481_length_30345_cov_24.746418"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1218)
                     /locus_tag="PL000_09645"
     CDS             complement(58..1218)
                     /locus_tag="PL000_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNC89207.1"
                     /translation="MTEVVIAGIGQTEVGEHWEISLRDLAYDAIHAAMQDAGGLRPQA
                     LFVGNMLAPNLSRQAHVGTLLADYAGLGGIEAVTVEAAGASGGAALRQGYLAIASGMV
                     DVALVVGVEKFTDKVGAEVDEALATIGDSDFEAVQGMTPTSQAALLMKRYMHENDVPA
                     DGFAGFALTAHANGAANKHAMFRKAIKPETYAKAEMVSDPINMFDMAPNADGAAALVL
                     TRRDLLPSNSKSQLVKIAGSAASSDTLALHDRKDILYFDTAQLSAGKAMKQAGIVLDQ
                     VNFFEYHDMFSIYAALQLEAVGFAIKGKGWKLAADGSIGLKGKIPCATMGGMKARGFP
                     GGASGVYQAIEAVIQLQGQAGANQIKDARYGLIQSLGGPASTAVSHVLQRLD"
     gene            complement(1231..2262)
                     /locus_tag="PL000_09650"
     CDS             complement(1231..2262)
                     /locus_tag="PL000_09650"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNC89208.1"
                     /translation="MQNPLFTIPYSLHTDMAFFDLPLPELKNYRPTREEPADFDAFWK
                     STLAETRVHPLDAKFEKVEYGLIAQETYDVTFNGFGGQPVKGWLILPAQGIRSQRTAP
                     LPVVVEFIGYGGGRQFGFNWLFWASAGYAHFVMDTRGQGSTWSSGDTPDLYADGGNAH
                     FPGSMTKGILEPQHYYYRRVFTDAVRAIEAARSHPEVDEARVAVTGGSQGGGISIAAA
                     GLVPEVAVAMPDVPFLCHYRRATEISDAYPYKEIAEYCHVHRDKVETVFNTLSYFDGV
                     NFAARAKAKTLFSTGLMDQVCPPSTVYAAYNHWAGEKDIKVYTYNGHEGGQSFHDLEK
                     LQFLKSIWK"
     gene            complement(2295..3371)
                     /locus_tag="PL000_09655"
     CDS             complement(2295..3371)
                     /locus_tag="PL000_09655"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HNC89209.1"
                     /translation="MTTPQPSPTLLKPVKPVGIVGYGAYVPRFRLPAKEVARVWTGKT
                     SGLPIKEKAVPGLDEDVITMSIEAARNAMARAQIDPTELRAVWVGSESHPYAVKPTST
                     LVAEAIGAVPNTQAADWEFACKAGTEALVAAMGLVGSGMGHYAMAIGMDTAQGKPGDA
                     LEYTAGAGGGAYIVGPAEESLAVINASYSYVTDTPDFWRREYQKYPEHGMRFTGEPAY
                     FKHITEAATHLMEASGTTAKDYKWAVFHQPNTKFPQRVAGQLGFSMEQIEPGLLVPII
                     GNTYAGAAMIGLTATLDIAQPGDRILVVSFGSGAGSDAFDITVTDKAPARQSLATKTR
                     EYIARRSEIDYATYVRYRGKLAMK"
     gene            complement(3476..4468)
                     /locus_tag="PL000_09660"
     CDS             complement(3476..4468)
                     /locus_tag="PL000_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89210.1"
                     /translation="MSNDNKNFIGIGIVLLACICGFMATVGIAAAWSQRFSKAPSVVY
                     SKITTFNIPGTIIEVRESCSDGQCIDACIQHVKNQGLESDLDLQNYHDEEVTLIYYNV
                     DENSIHDPRVPEVPENLLAFQQDKSTHEKIWNYFNVLIPREHQGNLVQFVVYTSQESS
                     GKHEDTITGNWILKYNILSAEDVIETTETLVYLYGYHLMLSPEQQIPWGEICHQEYLH
                     GCPTPESYANQFYLRFWKDIFAEWEEFDDNLQKREEDMGLFYTKYQDQFINEFAAEDP
                     VKDMVESWTEFMLYPRHTGDSIVDQKVNFFGEFPELVELREKVITGICTYEQTP"
     gene            complement(4775..5530)
                     /locus_tag="PL000_09665"
     CDS             complement(4775..5530)
                     /locus_tag="PL000_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC89211.1"
                     /translation="MRTVVLSDVHGNCLALETVMEDAKKKGFDQIVCNGDMIQSGPQP
                     HETVQLLRELKCPVVMGNSDAWLLTGIETDADSISEERRKKLDIVREWSLTKLNEEDR
                     AFINSFQPTITVELGGDKQLLAFHGSPTSFDQVLSPYTPEDEFQEALKPYAGHILTGG
                     HTHLQFTRRLRDSQNFFFNPGSVGVAVNPEHTNEQNLLDPWAEYAILTVEKSNMRLEF
                     VRASLDMDKMAEIYRASDRPFADVMMDQYLSYK"
     gene            complement(5534..6016)
                     /locus_tag="PL000_09670"
                     /pseudo
     CDS             complement(5534..6016)
                     /locus_tag="PL000_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015216.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
     gene            complement(6213..>6453)
                     /locus_tag="PL000_09675"
     CDS             complement(6213..>6453)
                     /locus_tag="PL000_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HNC89212.1"
                     /translation="NELAEIIVSVGLAQNMAALRALATEGIQRGHMSLHARQVAIAAG
                     ASGELIEKVAAQMVAEKVVRSDRAEEILKAMVSGK"
     assembly_gap    6454..6553
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6554..7705)
                     /locus_tag="PL000_09680"
     CDS             complement(<6554..7705)
                     /locus_tag="PL000_09680"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HNC89213.1"
                     /translation="MIEFERTSNLYLGQSTMTNSRIPGFYNLTLEERRAKLAEASDRT
                     PEDLLPWATGGLSAESADHMIENVVGLHSLPLGIGLNFMVNGRDVLVPFAVEEPSVVA
                     GASFMAKLARAGGGYTATTTEPLMIGQMQLINVVNMNEARLKIYEHKAELLALADAID
                     PVLKKFGGGARDLEVRTIEESAIGPFLVLHLIYDVRDAMGANAVNTACEKLAPQIEAI
                     TGGKVHLRILSNLADRRIARARCTIPVSELTVGFESFKGETVRDGIIAAYAFAASDPY
                     RAATHNKGIMNGVDSVVIATGNDWRAIEAGAHAYAARNGRYTSLSTWGKDKDGNLVGT
                     LEMPMAVGIVGGATKVHPAAQAAVKLMGIKTANELAEIIVSVGLAQNMAA"
     gene            7746..9431
                     /locus_tag="PL000_09685"
     CDS             7746..9431
                     /locus_tag="PL000_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNC89214.1"
                     /translation="MLLTREQLQERLVALHRASLELVKDVSLETLLERIVATACEQVD
                     ARYAALGVLDDEGKLAQFISVGLTDKEIKRIVHPPVGLGLIGALMNTETTIRIPVIHD
                     HPRSVGFPANHPLMNSFLGVPIRIGDRQLGQIYLTEKIDALEFSADDEMIIQMLASYA
                     ATAIKNARLYEQMKQRDLTLTRRNVDMSLLNGIASTLTSSLELDEILNKTLGLVMNYM
                     KVEAGDIFLLEEDKTTLRMVLHRGQAAEAFWTRNIFVVGEGYPGLVAKSRQPMIGRHL
                     ASDTNFLRDAIVQAGFQQIACIPLISGDNLMGVMSVATRANEPLDERNIQMLIAVGAW
                     AGLAIENARLHSNARRLAVLEERDRIGMDLHDGIIQSIYGVGLGLEGAQLMLNEDPQA
                     AKAHIRQAIDGLNQAIRDIRAYILDLRPRQLGNEGLLTGIKRLIAEYRAHTFSEVNFT
                     GPETDLKDLPHTHSLALFHICQEALANAAKHAKAKNVQVAIWTTNERALMEIRDDGKG
                     FDTENMHSSIGHGLANMQTRAHAVGGEFDISSIIDEGTTILVWVPRVIGRVIK"
     gene            9883..11220
                     /gene="dnaA"
                     /locus_tag="PL000_09690"
     CDS             9883..11220
                     /gene="dnaA"
                     /locus_tag="PL000_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC89215.1"
                     /translation="MNAEQAWQSVLAQLQMEMPRASFDTWVRDTRPVAYDNGVITVGV
                     RNAYARDWLESRLASSVNRLLIGILNSNVTVDFVVSQADEAESTPEAESAPATIEITA
                     PEPKTKHVTLNPRYTFDTYVVGSGNRLAHAACQAVAEKPARAYNPLFLYGGVGLGKTH
                     LLHATGNACHASGLNVLYVSSEEFTNDMITAIRTHTTQAFRDKYRSADVLLVDDIQFI
                     AGKESTQEEFFHTFNTLHGQDKQIIVSSDRPPKSLVTLEERLRSRFEWGLSADIQAPD
                     FETRLAILRSKAERTGRQISDEILESIAKQVQSNIRELEGALNRIIAFADLSGSALTP
                     SLVEVALSDLLPNRSDLEPEKIVTQVADYYKLSVEKLMGRDRTKDVAYSRQIAMYLLR
                     EVSKISFPQIGEALGGRDHSTVMSAIEKIKDQYKSGDGRVKKDVDFLTQKLYA"
     gene            complement(11252..12556)
                     /locus_tag="PL000_09695"
     CDS             complement(11252..12556)
                     /locus_tag="PL000_09695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNC89216.1"
                     /translation="MKPILKQLLFLFVLNLVFSFAIQGITNSLGLKNNKMAVDHAIWI
                     FTREIGDDSWRPMKMAFDHWKESQGQSLVYSELLLTSKVKFQYPPTALLITRFIENNQ
                     INLLAFSTATTFLFLLLMAASVIATSLYSYGKTDAPKLNAAEKVGFGALLILLLLTFY
                     PAVKAGTLGQIQVWLNAFFALAILCYFTGYETLAGILLGLMASIKPQYALFIFWGLLR
                     GNKRLVIAMIITGAVGLLLGVRVFGLAMYVDYFRGLSFLAQHGESYYSNQSFNGLAGR
                     IFGVKYPEVFNNTKWRGYYFPPYNTWIFAFTQVTSIAILLIGLWKTKSSTTEARSADF
                     LLMGLAATLASPIAWEHHYGILFPIFVCLGLFIWVGESFGKSLWVKIAFVTCYLLAAN
                     VIPFTKFVADSYLNILQSYLFFAAGGAFILLAMVKHQFPYKK"
     gene            12767..13654
                     /gene="rpsB"
                     /locus_tag="PL000_09700"
     CDS             12767..13654
                     /gene="rpsB"
                     /locus_tag="PL000_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC89217.1"
                     /translation="MAVISMKALLESGVHFGHRTNKWDPRMKPYIFTERNGIHIIDLQ
                     QTVKLLNQGYNVIRDTVANGGTVMFVGTKRQAQETVAEEAIRCGMPYVTERWMGGMLT
                     NWSTMYQRIQELERLEKVRDSDEINRLTKKEGLLIEREITRLSTRLSGARSLKRRPDL
                     LFIIDVGREEAAVREANLLKIPIVALVDTNCNPQNVDYVIPSNDDAIRAIKLLVAKVA
                     DAVLEGKAMRKEEEPEQPASADAQSEKPKTRGPRKPVVDVDTEEVGEDSLLGASTLAK
                     LNVSRKGDDVAADDTAAAE"
     gene            13693..14301
                     /gene="tsf"
                     /locus_tag="PL000_09705"
     CDS             13693..14301
                     /gene="tsf"
                     /locus_tag="PL000_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNC89218.1"
                     /translation="MEITTQMIKDLRAATSAPMLDCRKALQEANGDFQKAVDWLREKG
                     MATAAKRSDRVASNGIVEMYSHGGGRVGVMVEVNCETDFVARAEQFRSLAHEIALQIA
                     ASAPKYITAEDIPAAELAHEADIARARAIEEGKPEKMLDKIVEGRVEKYKDEVCLLRQ
                     TYIRDESLTIEKLILQNVAAIGESVIVRRFQRWELGESAASN"
     gene            14369..15085
                     /gene="pyrH"
                     /locus_tag="PL000_09710"
     CDS             14369..15085
                     /gene="pyrH"
                     /locus_tag="PL000_09710"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC89219.1"
                     /translation="MAALKYKRILLKLSGEALGGKSGFGIDVDEAESIASRIKEVHEM
                     GVDVAVVIGAGNLWRGKQGLERGMDRSTADYMGMLATVMNAMSLMDALERQNVFTRVM
                     SAIEMRAIAEPYIRRRAVRHLEKRRVVIFGAGTGNPFFSTDTAAALRATEIDADVVIK
                     ATKVDGVYDADPKKNPDAKKFDQLSYIEVLNRRLEVMDSTAITLCMENNLPILVLNLW
                     DTKALLGALRGEKVGTLVSS"
     gene            15268..15837
                     /gene="frr"
                     /locus_tag="PL000_09715"
     CDS             15268..15837
                     /gene="frr"
                     /locus_tag="PL000_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNC89220.1"
                     /translation="MSTDEIRDLLKDAETRMSGAIHALTDDLSGIRTGRASPALVERL
                     SVEYYGTPTHLMQLATISVPEPRTLMIKPFDAGSVKDIERAIRASNLGLNPNSDGKVI
                     HLNLPPLDEERRRDLVKQMSHRLEEARVAVRNIRRDLHNDLRDYEKEKLITEDDLKRG
                     EEDLQKLTDKFIEDIARHGGNKEKEIMEV"
     gene            15883..16629
                     /locus_tag="PL000_09720"
     CDS             15883..16629
                     /locus_tag="PL000_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HNC89221.1"
                     /translation="MAEIPSAIPLEKIPQHVAMIMDGNGRWALQRGLPRLAGHKAGTE
                     NLRRVIRASVEFGVKYLTIYAFSTENWGRPPEEVKGLMYILEDVIDRELNELNKEGVQ
                     LRHIGRLERLAPSLQEKVLDAIEATKNNNRLVLNVAFNYGGRDEIVLAIQKMMKDGIA
                     PEQVTDELVSQYLFTAGVPDPDLIIRTSGELRISNFLIWQAAYAEWYITPTFWPDFDR
                     EEYHRALEAFAHRDRRYGKVSSGELQESNA"
     gene            16622..17434
                     /locus_tag="PL000_09725"
     CDS             16622..17434
                     /locus_tag="PL000_09725"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC89222.1"
                     /translation="MRRRLITALGLAVIGVPAILLGGIFYYLLMGTFLIGAAWEYVHM
                     FRAVKFEPQMHLTVGGVALVTLARMFAPEFAMPVFGAAILAALTYHLIAYERGRDQAA
                     LDFCISIGGVVYIGWIGSYLFDLRNLPQGGWWFMLVMFCVWAGDSGAYSIGRAYGKHK
                     MAPRLSPKKSWEGYVASVFTGIITGGFYVWVFQRFGSLTHDISIWQGAILGLLLGAFP
                     TLGDLGESMIKRQSGLKDSSDIIPGHGGFFDRIDSWLWGSMIGFYFLVWFIL"
     gene            17451..18443
                     /gene="glpX"
                     /locus_tag="PL000_09730"
     CDS             17451..18443
                     /gene="glpX"
                     /locus_tag="PL000_09730"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNC89223.1"
                     /translation="MEVRGATPTRNLALELARVTEAAAMLAGRYMGRGDKEGADQAAV
                     NAMRLVLSTVDMNGVIVIGEGEKDKAPMLFNGEKVGNGSEPDVDVAVDPIDGTRPLAF
                     GRSNSLAMVALAPRGTMFDPGPFLYMNKLAVGPEAKGKIDIEKPISENLKAIAKAKGK
                     QIEDLTTIILDRPRHDDMVAEIRKAGARIRQIPDGDVAAALMTSWPDSGVDVLLGIGG
                     TPEGVITACALRAMGGEIQGKLFARDEDELRRGREAGYDFEKVLTMDDLVSSEDVFFA
                     TTGITDGELLQGVRYYGNHITTDTLVVRGLTGTIRRISATHSTDKLDNLSSVHY"
     gene            18490..18565
                     /locus_tag="PL000_09735"
     tRNA            18490..18565
                     /locus_tag="PL000_09735"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18523..18525,aa:Asn,seq:gtt)
     gene            complement(18662..19513)
                     /locus_tag="PL000_09740"
     CDS             complement(18662..19513)
                     /locus_tag="PL000_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89224.1"
                     /translation="MEITTLSQTNWQTQLRALYVIARKDWKVFWRYPLNAVSSIFQPL
                     IWVTPVYFMGKAFSTNGQALGFAGYTGSGDYMSYILIGTALSNFIMTVFWGMGYALKE
                     DMDAGVLESNWLTPISRLHILVGRTLTSLLTTTITSTVMLIIGALLFGFKPTGNIIAA
                     ILTAIPMLIGLYGFGFAFAAIVLVMRDANTMVDVSSFLVQGLTGANFPVKSLPYWLIP
                     ISLMLPLTYGFDAVRGWILKTNTLLPLPVEVALLIGTMFLMLWFGSWFFYRVERNVRS
                     RGTLGQH"
     gene            complement(19523..20392)
                     /locus_tag="PL000_09745"
     CDS             complement(19523..20392)
                     /locus_tag="PL000_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89225.1"
                     /translation="MQTTLSPQSLGRPGFASDLRALWAVVRREWTITMRYPSWIIAMF
                     IWPLIFPMMYILTAHALSGPDGSGLQVFLNTTGATEYIGYIVVGTTVWMWQNIVLWDV
                     GFALRQEQMRGTLESNWLSPTWRFAYLLGHTGPQLVSMLLFVSITAFEFGVFFGVRLN
                     GNPWMIGLMMLAAIPAIYGLGFAFASVVITMKEANAFVFLVRGLVMIFCGITFPVSLL
                     PGWMQGIAKFMPQTYLIHGMRAAAFNNAGIPELAPDLIPILLFGLFWLALGYSLFVWM
                     ERRARITGAIGQY"
     gene            complement(20394..21452)
                     /locus_tag="PL000_09750"
     CDS             complement(20394..21452)
                     /locus_tag="PL000_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC89226.1"
                     /translation="MSDFAIEATQLVKKFPARPGTGDKAVETESAQGSKKKRWQFWKK
                     EPQAMFTAVNGVDLQIRRGEIFGLLGPNGAGKSTTIRMLCTLLEPTSGTAKVNGFDVV
                     AQANDVRRSLGTVLAGERSIYWKLTGRENLEYFAALYHIPAAITKKRVDDLLERMEIK
                     DRANELVEKYSTGMRQRIAIAKALLARPPILLLDEPTLGLDPQAARNLRELIATLKAE
                     GHTILLTTHYMEEADQLSDRIGIIDTGKVIALDTPEGLKRKIDQREVIKLELTGWHDD
                     LSDKLKGIAGIETLTTRKQGEADLWEVNMQAQNSRTALPRVVESLSGNGTRLVNMNIV
                     KPSLEDVFIHLTGKALRD"
     gene            21731..22726
                     /locus_tag="PL000_09755"
     CDS             21731..22726
                     /locus_tag="PL000_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89227.1"
                     /translation="MKILKERKGLFVKILVSVLFVFLCYGLSEKAFHWAVCGEKERKE
                     LDMLFLMFCEKEADAWFEKRFWEECTKNDKNLPPLTELLISACKEPAVRGFSDGEKLF
                     VIEGKTDEVYFLDLRTGERAPVPDGKQLWDEVFLSPDLVWFDGYLKPGDTGYIPHYVL
                     DLTDGKKYELFDLSWMPLKDGKFDTQNYGYIKSAEHVYFSPKENAVIALAPNFRQSPR
                     QNVIYFDSSLEKLLNDLGVNYELVDYSLADADVPSPTGKYVVRNDGNGIYFSGTNLPI
                     ITGAYSSSCFKSWYYDESAVVIACGISGYLYHNSITHSVSHPIPLPVLKLFLPTP"
     gene            complement(22799..23602)
                     /locus_tag="PL000_09760"
     CDS             complement(22799..23602)
                     /locus_tag="PL000_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89228.1"
                     /translation="MKIHPVLTLTLVLILAACNVPSSAPTNAPAEAAPAVEAATATAL
                     EVAAPTETAIPPTPIPPTETFTPTLPPPTASSTSTPTEIPTVDSLKAVVTADTLSCRY
                     GPGAEYLYFYGFRKDFKLTLIGRAHASNWVMVDGLEHKCWVNQKFISVEGDPQTLRVT
                     YPGEYELPESPYYAPPAVLSAENNGGTVTVKWTDVELRAGDEEDADMFIYIIEVWRCE
                     GGQIVFEALASNYPEVSFVDEGGCAEPSHGRVFFQEKHGFAGPAEIPWP"
     gene            complement(23605..24120)
                     /locus_tag="PL000_09765"
     CDS             complement(23605..24120)
                     /locus_tag="PL000_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89229.1"
                     /translation="MANSRNKFDTKDSLELGEHAENLFIVLAVRSGWKVSASSKDENI
                     DDHWDYLIEKAGEVFKVEVKSQKRIRRGDKTEQTDFTWVELRNVRGKVGWLFGKADLI
                     AFEKESSFLFVKRLDLLAVVNKKVNLVAKVRTPADAVYKIYIREGRKDKITLLPTSDV
                     EEIKFMEWKKT"
     gene            complement(24122..24916)
                     /locus_tag="PL000_09770"
     CDS             complement(24122..24916)
                     /locus_tag="PL000_09770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC89230.1"
                     /translation="MKAFGIHFQNERFTLVHDDILTTSLVSANSVDLVVTSPPYNVDI
                     QYNSHKDDVSYADYLEFSKKWMSRCFDWLKDDGRFCLNVPLDKNKGGQQSVGADLTTI
                     AKQIGFNYHSTIIWNEGNISRRTAWGSWKSASAPYVIAPVELIIVLYKNNWKKTSGSK
                     ISDIERNEFMDWTNGLWTFNGESKKRIGHPAPFPLELPRRCIKLFSYVDDVVLDPFSG
                     SGTTLLAAVNNNRKGIGIDVDRKYCELARKRILGITPAPVKPKKKK"
     gene            complement(24938..25711)
                     /locus_tag="PL000_09775"
     CDS             complement(24938..25711)
                     /locus_tag="PL000_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNC89231.1"
                     /translation="MIEEQPPLENQPTPETQNEPVIVEPQKTNWRVWLAAGGCAVLLC
                     AAVFVGTLIFVGPKFVQQFFPDKVEVAEELPRAVIQANTMGDPHAPIHIIEYGDFQCP
                     YCLRFWNETEPLLIEEYVNTGKVYFEYRSFPFLGPESVLAAEGAYCAGDQGMFWEYHD
                     TLYANWTGENVGDFTQENLIKYAKAVNLDVDAFETCLSEGTHKGTVEQDKAQAEADGV
                     HATPTFLINGHILEGAQPFSVFKQFIEKILEGNFDSQSG"
     gene            25929..27800
                     /locus_tag="PL000_09780"
     CDS             25929..27800
                     /locus_tag="PL000_09780"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNC89232.1"
                     /translation="MNATLTSNQNLPKQIVAALVGGFSLFLTVTITWTLGYQLLYAGR
                     IFPGVSVAGVDVSGLSINDAALKLSQTLSFPNTGKILFRAGDKLWVASPAELGMVFDP
                     TASALAAYRYGRSGGLFGALSGQVSARGLGSDVAPVIIFDQRVAYTYLQNIGAQVNQP
                     VVEASLHLEGTNVVAQPGQVGRVLNLDATLIYLGAQLQTFRDGEVPLVIQEAAPKLLD
                     VSSQAEAARRILSQPLTLSLPNYRSGDPGPWTYDVPVVANMIAVIVVENGGGAEMQVG
                     LDPNILRQSLNELKTYVDRQPTNAKFVFNDGTSQLEPIAASNVGRAMDVEASIAAIND
                     ALLRGEHNVSLVVAEQQPLVADTAVGADLGIVQLIAEQTTYFYGSSTERRQNIEAAAS
                     RFHGVLVAPGETFSMGSVMGDVSLENGFAEALIIYGGRTIKGVGGGVCQVSTTLFRTV
                     FFAGFPVVERYSHAYRVSYYEKTSSGSVDSNLAGMDATVYFPLVDFKFQNDTPYWILM
                     ETYMSENSLTWKLYSTSDGRSITWETTGPTNIVPAPPPLLEENPEMKAEQIKQVDYAA
                     QGADVNVTRVVWRNGVIYFTDQFITHYQPWQAICQYGPGTEDPKKLARRNDICVGAS"
     gene            27963..28181
                     /locus_tag="PL000_09785"
     CDS             27963..28181
                     /locus_tag="PL000_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89233.1"
                     /translation="MVSSELLEILRCPNCVREKSGTLVLHKDAWLICQDCGRKYPIVD
                     DIPVMLIDEGDKWVNTAQDALPVPPPEK"
     gene            28190..28768
                     /locus_tag="PL000_09790"
     CDS             28190..28768
                     /locus_tag="PL000_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC89234.1"
                     /translation="MSETKMQELIAELLSGDDERAEKTIPALVGFGMAAHSALLDLTR
                     AADVDSRWWGVRALAASPHTQTEDLLPLLSDSAPEVRAATALALCNHPHEAAVEALVQ
                     ALADEDPLTAGLAGTALGKIGAPSVPSLLKVMSEAPVGVKILALRALSEIKDHRAIPV
                     MMKSMGEESALLQYWAQEGLERLGLNMVYVKP"
     gene            28782..30245
                     /locus_tag="PL000_09795"
     CDS             28782..30245
                     /locus_tag="PL000_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNC89235.1"
                     /translation="MNRFEFLKKIKLPKPKIPSVGRIIFWAISIGLAAATFVFARNFT
                     SCWNITGLPGIAPSSCGTSTNPLAGPDLTAATQEPGAVDTLPTPAPVAPGVELPPAWD
                     GGSRINILFIGLDFRDWQANDGPPRSDTMILFTIDPVTKSAGMLSIPRDLWVNIPEGF
                     GYSRINTAYSSGEGAKLPGGGPGLAMKTVEQLLGVPVHYYAQVDFNTFTELVNRLGCI
                     YVVPEEILVLDPVGGGLDHVVITPGGERKLCGWKVLAYARTRKTEGGDMDRARRQQQV
                     IFALQQKIFDPEVFPSLIAQAPEMYAELSSGIHTNMPLDTAIKLAILGKDITRESIKT
                     GIIGTDMVTFESTTLGGQAASVMKPIMDKVRVLRDEIFTSGGPTSPMAQGEPAALMRE
                     EEARVRVVDGTFTTGLDQRAGSFFQSQGMNVTEVGPASEIYSSTFIIVYGPKLYTVKY
                     LQAVFGIPASNIRFSPDPASTVDIEIRLGSDLAGSIP"
BASE COUNT         7150 a         7566 c         7996 g         7533 t
ORIGIN      
        1 gaaaatggac ggtagatagt ggcttttcaa aaagaaacac tgtctgccgt ccatcggtta
       61 atccaatctt tgcagaacat ggctgaccgc tgtggatgct ggcccgccca gcgactggat
      121 caacccgtag cgtgcatcct tgatctgatt cgctcctgcc tgaccttgta actggatcac
      181 ggcttcgatg gcttggtata ccccggaagc gccgccgggg aatccgcggg ctttcatgcc
      241 gcccattgtg gcgcaaggga ttttcccttt caatccgatc gaaccatcgg cggctaactt
      301 ccagcctttg cctttgatgg cgaatccgac tgcttcgagc tgcagggcgg cgtaaatgct
      361 gaacatgtcg tggtattcga agaagttgac ctgatcgagc acaatgcctg cctgtttcat
      421 ggctttccct gcggagagtt gagccgtgtc aaaatacaga atgtcttttc tgtcgtgcaa
      481 tgccagtgtg tctgatgagg cggcggagcc agcgatcttt acgagctgac tctttgaatt
      541 ggacgggagc aggtcacggc gagtcagaac gagcgcggca gcgccatccg cattgggggc
      601 catgtcgaac atattgatcg ggtcgctgac catttccgct ttggcgtagg tttcgggttt
      661 tatggctttg cggaacatgg catgtttgtt ggcggcgccg ttggcgtgtg cggtgagtgc
      721 aaagcctgca aaaccatcgg cagggacatc gttctcatgc atgtagcgtt tcatcaaaag
      781 cgcggcttgt gaggtgggag tcatgccttg cacggcttca aaatcagaat cgccgatggt
      841 ggcgagggcc tcatccactt ctgcgccgac tttgtctgta aatttttcca caccgaccac
      901 gagggctacg tccaccattc cgcttgcaat ggcgagataa ccctggcgga gtgctgcacc
      961 gcctgatgcg cccgccgctt ccactgtgac ggcttcaatg ccgcccaacc ctgcatagtc
     1021 agccaacaat gtgccgacat gggcctgccg cgaaaggttc ggcgcgagca tgttgcccac
     1081 gaacagtgct tgtggtctga ggccgcccgc atcttgcatg gcggcgtgaa tggcatcgta
     1141 ggcaagatcg cgcaagctga tctcccaatg ttcgccgact tcggtttgtc caattcctgc
     1201 gataacgact tctgtcatga atcctctagc ttatttccaa atacttttca gaaactgtaa
     1261 tttctcaagg tcgtgaaaac tttgtccgcc ttcatgaccg ttgtaggtat agaccttgat
     1321 atctttttcg ccagcccaat ggttgtaggc tgcatacacg gttgaaggtg ggcagacctg
     1381 atccatcaag ccagtggaaa ataaagtttt ggctttggcc cgtgcggcaa aattcactcc
     1441 gtcaaaatag gaaagcgtgt tgaacacagt ttccaccttg tcacgatgaa catggcaata
     1501 ttcggcgatc tctttgtagg gatacgcatc gctgatctcg gtggcacggc ggtaatgaca
     1561 aaggaacggc acatcgggca tggctacggc gacctcgggt acaagccctg ccgcggcgat
     1621 ggagattcct ccgccttgag agccgcctgt cactgcgaca cgcgcttcat ccacttcggg
     1681 atggctgcgt gctgcttcga tggcacgcac agcgtcggtg aagactctgc gatagtaata
     1741 gtgctgtggt tcaaggatgc ctttggtcat actgcctgga aaatgggcat tgccgccatc
     1801 ggcatataga tcaggggtgt cacctgatga ccatgtgctg ccttgtccgc gggtatccat
     1861 gacgaagtga gcgtaacctg cacttgccca aaacagccaa ttgaatccaa attgacgtcc
     1921 gccgccgtag ccgatgaact cgacaacgac gggtaggggt gcagtacgct gagaccttat
     1981 gccttgtgcg gggagaatca accagccttt gacgggttgt ccaccgaagc cattgaaggt
     2041 tacatcgtag gtttcctgcg cgataaggcc gtattccact ttttcaaatt ttgcgtcgag
     2101 aggatgtacc cgcgtttcgg cgagggtgga tttccaaaac gcgtcaaagt ccgcgggttc
     2161 ttcacgcgtg gggcggtagt tttttagctc ggggagggga agatcgaaga aggccatgtc
     2221 agtatgtagt gaatagggga tagtgaatag tgggttttgc aaatcccatt cactagctcc
     2281 tattccctat ccttttattt catcgccagc ttgccgcgat accgcacata ggtggcgtaa
     2341 tcaatttcac tgcggcgggc gatgtattcg cgggtttttg tggcaagact ctggcgggcg
     2401 ggggctttat cggtgacggt gatgtcaaat gcgtctgacc ctgccccaga gccgaaagag
     2461 acgacgagga tcctgtcacc gggttgggcg atatcgagag tcgcggtcag gccgatcatg
     2521 gctgcgcctg catacgtgtt tccgatgatg ggaaccaaca gtccgggctc gatctgctcc
     2581 attgaaaatc caagctgccc cgcaacacgt tgcgggaatt tggtgttcgg ctggtggaag
     2641 accgcccatt tgtaatcttt ggcggtggtg ccgctggctt ccataagatg ggtggcggct
     2701 tcggtgatgt gtttgaagta ggcaggttcg cccgtgaagc gcatgccgtg ttcgggatat
     2761 ttctgatatt cacgccgcca aaaatcgggt gtatcggtga cgtaggaata cgaagcattg
     2821 atgaccgcca atgactcttc ggctgggcct acgatatatg cgccgccacc tgcgcctgcg
     2881 gtgtattcaa gcgcgtcgcc gggtttgcct tgggcggtgt ccatgccgat ggccatggcg
     2941 tagtgtccca tgcctgagcc gaccaaaccc atcgcagcga cgagggcttc ggtgcctgct
     3001 ttacaggcga attcccaatc cgcggcctgg gtgttgggta cggcgccgat cgcttctgcg
     3061 accagcgttg aagtgggttt caccgcatac ggatgcgatt cggacccgac ccacaccgca
     3121 cggagttcag tcgggtcaat ttgcgcacgc gccatcgcat tccgcgccgc ttcgatggac
     3181 atggtgatca cgtcttcatc gagcccgggt acggctttct ctttgatggg gagcccgctg
     3241 gttttacctg tccatacacg cgcaacttcc ttcgcgggca agcggaagcg cggaacataa
     3301 gcgccatacc cgacaatgcc aacaggtttg acgggtttga ggagggtggg ggagggttgg
     3361 ggagttgtca ttggataacc tttgaataga ttgctctcat tctatgggtg gaaggcttgc
     3421 aaaacctatc ttgttggtcc ggaaaggttg catattgaaa gataaaaccg tttttttagg
     3481 gtgtctgttc gtacgtgcaa attccagtta tgaccttttc acggagttca accaattcgg
     3541 gaaattcgcc gaaaaaatta actttttgat ctacgatcga atcgccggta tgacgtgggt
     3601 ataacataaa ttccgtccat gactcaacca tgtcttttac aggatcttcc gcggcaaact
     3661 cgttaataaa ctgatcctgg tattttgtgt aaaacagtcc catatcttct tcgcgctttt
     3721 ggaggttgtc atcaaactcc tcccattcag cgaaaatatc cttccagaat ctcaggtaaa
     3781 actgattcgc atatgactct ggggttgggc atccatgcag atattcctga tggcaaattt
     3841 ctccccacgg aatttgttgt tccgggctta gcatgagatg atatccgtat aggtaaacca
     3901 aggtctctgt cgtttcgatt acatcttctg cagaaaggat gttgtatttg agtatccagt
     3961 ttcctgttat tgtatcttca tgcttgccgc ttgattcctg actggtgtag actacaaatt
     4021 gaactaggtt cccctgatgt tcacgaggta tgagtacgtt gaaataattc cagatctttt
     4081 cgtgcgtgga tttatcctgt tgaaaagcta gcaggttttc aggtacttct ggcacacgag
     4141 ggtcgtggat cgaattttcg tcgacgttgt aataaatcaa cgtaacctct tcatcgtggt
     4201 agttttgcaa atcaagatca ctttccagac cttgattctt aacatgctgt atacaggcat
     4261 ctatacattg accatcggaa catgactccc tgacttcaat gattgtacct ggaatattga
     4321 acgtggttat ctttgaataa accacagacg gggctttcga aaacctttgg gaccaggcgg
     4381 ctgcaatgcc aaccgttgcc ataaacccgc aaatacaagc gagtagaacg attcctattc
     4441 caatgaaatt tttgttgtcg tttgacatat tgacatacct gttttagttc agattagtat
     4501 cccttgaaaa accctctgct caggttcgat atgaagccat atttgcaatg tgtggtcttt
     4561 tggtaattgg cagcgaattt tgacttctaa ccgcctgagg gtgtttcatc ataattgatg
     4621 aaacacgtat tttggataag tccattacct ttcaggatct ccaaaatatc atcaacgatt
     4681 ttgctcatgt tgcaaatcct gacttgaata taccaaaact ttgacgtcaa ccctgccagc
     4741 attgaaatct ctgcaacctt ggcaggctta tttcctattt gtaagataaa tattgatcca
     4801 tcattacgtc cgcaaaaggc ctgtcgcttg cgcgatagat ttcggccatc ttatccatat
     4861 ccaatgaagc gcggacaaac tccagccgca tgttggattt ttcgaccgtt agaatggcgt
     4921 attctgccca gggatcgagt agattttgtt cgttcgtatg ttcgggattg acggcaacac
     4981 cgacgctgcc ggggttgaaa aagaaattct ggctgtcgcg caatcggcgc gtaaattgaa
     5041 gatgcgtgtg cccaccggtg aggatatgac ctgcatacgg tttgagagct tcttgaaatt
     5101 catcttctgg tgtgtaagga gaaaggacct ggtcgaacga tgtgggcgag ccgtgaaaag
     5161 ccagtagttg tttatcgcct cccaattcaa ccgtgatggt cggttgaaag gaattgataa
     5221 aagcccggtc ttcttcgttt agcttggtca atgaccactc gcggacaata tccagtttct
     5281 tgcgccgttc ttccgatatc gaatctgcat cggtctcgat cccggtcaac agccatgcat
     5341 cggaattacc catcacgact gggcatttca actcgcgtag gagttggaca gtctcatgtg
     5401 gctgcggtcc gctttgaatc atatcgccat tacagacaat ctggtcgaag cctttctttt
     5461 tggcatcttc cattacggtt tcgagcgcca ggcaattgcc atgaacatca gataaaacta
     5521 ctgttctcat ttttcaactc cgtggatacc aaaacttgga agtcaacccg acctgcctga
     5581 aaacctctgc aacctgctca atgctccgcc catgaaaatc cacggatagg cggttactgt
     5641 accagagttt cgagagttcc catacttgat cgatcgtcag gatttccccg cgagcgaggc
     5701 ggtttcgctt gcaccacttg tcaatccatt cttccgaccg gaagagcagc atggtggctc
     5761 aagtgtagac taggtcgtca taccagcgtg aaaaagaaag tggaaagtgg acaagtaatt
     5821 cgctgtttgt caccttgccg cctttgactt ccagttgaac caaatcgttg ctttcggtgc
     5881 aaaccgcttc gatgatcgcg tcacagtgta gcgcggcggg gattccgagc atgtcccagg
     5941 cgcaattggc aaagtaggtc ttcccattgg catggacttt gaagtcagta ggaatgcccg
     6001 agaagggatt tgccatgcgg atggtcaggg tctctggctc gaggaaaaag gaatgacggt
     6061 tgttcaattc cttgtagatt tcgccagctt cttcagtttt gatgttaaag tgcgcagcgg
     6121 tctcttccac gctgggtggg tgggttgtgt ctgcaatgtg ggtgtaaacg aagtgtcgta
     6181 cctgccagag gagatcttcg tctttcatct ttttactttc cacttaccat tgccttcaaa
     6241 atttcttcag cccgatcact tcttacaacc ttttccgcca ccatttgtgc ggcaactttt
     6301 tcgatcaatt cgccagatgc gccagctgca atggcaacct gtcttgcgtg tagcgacatg
     6361 tggcctctct gaatgccttc tgtagcaagg gcacgcaggg cggccatgtt ttgagcaagc
     6421 cctacagaaa cgatgatctc agccagttcg ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnggcggcc atgttttgag caagccctac agaaacgatg atctcagcca
     6601 gttcgttggc cgttttgatg cccattaatt taactgcggc ctgagcagca gggtggacct
     6661 tggtcgcccc gccaacgatc cctactgcca ttggcatttc aagcgtgcca acgaggttgc
     6721 catctttgtc tttgccccaa gtggagaggg aagtgtaacg tccgttgcgg gccgcgtagg
     6781 cgtgagcacc tgcttcaatg gcgcgccaat cgttgcccgt agcgatgact accgagtcta
     6841 cgccgttcat aattcccttg ttgtgggttg cggcgcggta tggatcggat gcggcgaagg
     6901 catatgcggc gataatgcca tcacgtacgg tctcaccttt gaacgattca aacccgaccg
     6961 tgagttcgct cacaggaatg gtacagcgtg cgcgtgcaat tctgcggtct gccaggttgg
     7021 acaaaatacg caagtggact tttccgcctg tgatggcttc gatctgtgga gcgagctttt
     7081 cgcaggctgt attcactgca tttgcgccca tggcatcgcg cacatcataa atgaggtgca
     7141 ggacgaggaa tggcccgatg gccgattctt cgatggtccg cacttcgagg tcgcgggctc
     7201 cgccgccgaa tttcttcaac acagggtcaa tggcatcggc cagggcgagt aattcggctt
     7261 tgtgttcgta aatttttaat cgcgcctcgt tcatgttgac cacattgatg agctgcatct
     7321 gtccgatcat caatggttcg gtggtggtgg ccgtgtatcc accgccggcg cgggcgagtt
     7381 tggccatgaa tgatgccccg gccacaaccg agggttcctc cacggcaaac ggaaccagaa
     7441 catcgcgtcc atttaccata aagttgaggc cgatgccgag gggcagggaa tgcaagccca
     7501 ccacgttctc aatcatgtgg tcggctgatt cagcggagag tccgcctgtt gcccacggga
     7561 gcaggtcttc gggagtccga tccgaggctt ccgctagttt ggcgcggcgt tcttcgaggg
     7621 tcaggttgta aaatccgggg atgcgcgaat tggtcatggt tgattgtccc aagtataggt
     7681 ttgacgttct ttcaaattct atcaaaaagc gatagattga ttataatgtc tgggaggtat
     7741 cttttatgct attaacccgt gaacagttgc aggagcgttt ggtcgcgctt cacagagcca
     7801 gccttgaact ggtcaaggat gtttcgcttg aaactctgct cgaacggatc gtggccacgg
     7861 cttgcgaaca ggttgatgcg cgttatgcag ctttgggcgt tttggatgat gaggggaaac
     7921 ttgcccagtt tatttcagtc ggcctgacag ataaagagat caaacgtatc gtgcatcctc
     7981 ccgttgggct tgggctgatc ggtgcgttga tgaatactga aaccaccatc cgcattccgg
     8041 tcatccatga ccacccgcgg tcagtcggat tccccgcaaa tcacccgctc atgaactcat
     8101 ttttaggggt tcccatccgc atcggtgaca ggcagttggg gcaaatttat ctcactgaaa
     8161 aaatagatgc actggaattc agcgccgacg atgagatgat cattcaaatg ctggcctcgt
     8221 atgccgcgac tgccatcaaa aacgcgcgat tatacgagca gatgaaacaa cgcgacctga
     8281 ctctgacgcg ccgcaatgtg gatatgtctc tgctgaatgg catcgcatcc accttaacct
     8341 caagcctgga actcgacgag attctgaaca agacccttgg gcttgtgatg aactacatga
     8401 aagtggaagc gggtgatatt ttcctgcttg aagaagataa aaccaccctg cgtatggttc
     8461 tgcaccgtgg gcaagccgcc gaagcctttt ggacgcgtaa catctttgtg gtgggtgagg
     8521 gttaccctgg ccttgttgcc aaaagccgcc aacccatgat cggcaggcac cttgcaagtg
     8581 ataccaactt tttgcgagat gcaatcgttc aggcaggttt tcaacaaatt gcgtgtattc
     8641 cgttgatttc aggtgataac ctcatgggag ttatgagtgt tgccacccgt gccaatgagc
     8701 ctttggatga aagaaacatt caaatgctga tcgcagtggg agcttgggcg ggtcttgcga
     8761 tagaaaacgc ccgtttacat tccaatgcgc gccgtttggc cgtgctggaa gaacgtgacc
     8821 gcatcggcat ggatttgcac gacggcatta ttcagtcaat ttatggcgtg ggattggggt
     8881 tggaaggcgc ccaattgatg ctaaatgagg atccacaggc tgcaaaagcc catattcgtc
     8941 aggctattga tggattaaat caggcaattc gtgatatccg cgcttatatt cttgatttga
     9001 gaccacgtca gcttggtaat gaaggcttat taacgggaat caaacgcctg atcgcagaat
     9061 atcgcgctca cacgttctct gaagtgaact tcaccggccc cgaaacagac ttaaaagacc
     9121 tgccacatac tcattcactg gcattgtttc atatatgtca ggaggctttg gccaatgcag
     9181 ccaaacatgc caaggcaaaa aatgtacaag tagcaatatg gacaaccaat gaacgcgccc
     9241 tgatggagat cagagatgat ggtaaaggct ttgatacaga gaatatgcac agttccattg
     9301 ggcatggtct cgccaacatg cagactcgcg cacatgctgt aggcggtgaa ttcgatatat
     9361 catcaataat tgacgaggga accaccattc ttgtatgggt tccacgtgtt attggacgcg
     9421 ttatcaaatg aatcaaatca tcttgcaatt gaatcccgga aatttgagtc tcgtttaata
     9481 tgttttaaaa acgtttcttc tttcgatttt ttaagataga tatatgtcag gaaccatttt
     9541 atagcccata gcacccatat atggggggtg ggaagcccca aaacggtgtg tttggctgaa
     9601 acgcctgttc tatctcaaat cataatcgtg tttgaatgca agatatttca taaacgcatg
     9661 tcaaccttaa aaaaacaaat ctttttaact taacgtaggt gtaacgcctt gttatctaaa
     9721 gaaagtatct gctagaatgt aggcacgctg ataaaatgga ttgatgccca ttgcatccag
     9781 atccctgaaa aaaatttaca aactaatatg gtttccccaa ataagccgca gaacaaagcg
     9841 ggctggattt ctgtacgata aagtcattat caaggaatgg ttatgaacgc tgagcaggcc
     9901 tggcaatcag tactcgcaca acttcaaatg gaaatgcccc gcgcatcttt tgatacctgg
     9961 gtgcgcgata cccgtccggt tgcatacgac aacggtgtta ttactgttgg cgtacgcaat
    10021 gcctatgccc gtgactggct ggaaagtcgc cttgcgagta gtgtcaatcg cctgttgatc
    10081 ggtattttga attcgaatgt gaccgtggat tttgttgttt ctcaagctga tgaagccgaa
    10141 tccaccccgg aagccgaatc tgctcctgcc acgatcgaaa ttaccgcccc tgaaccaaaa
    10201 acaaagcatg tcaccctgaa tccacgctat acctttgaca cttatgtcgt tggttcgggt
    10261 aaccgcctcg cccatgcggc ttgtcaggcg gtggccgaga agcccgctcg tgcctacaat
    10321 ccgctttttc tgtatggcgg tgtagggctg ggaaaaaccc acctgttaca tgccactggt
    10381 aatgcctgcc atgccagcgg attaaatgtt ttatacgttt cctctgaaga attcacgaat
    10441 gacatgatca ccgccattcg tacccacacc acacaagcct tccgtgataa atatcgctct
    10501 gccgacgtat tgcttgtgga tgatattcag ttcattgccg gcaaagaatc cacgcaggaa
    10561 gagttcttcc acaccttcaa caccctgcat ggacaggata aacagatcat cgtttcgtct
    10621 gaccgacctc ccaaatcgct cgtcactttg gaagaacgtc tgcgatcccg cttcgagtgg
    10681 ggattaagtg ccgatattca agcgcctgat ttcgaaaccc gcctcgccat tttgcgctcc
    10741 aaagccgagc gcacgggccg ccagatctca gatgaaattc tcgaaagtat tgccaaacaa
    10801 gtgcagtcca acattcgcga attggaaggc gccctgaacc gtattatcgc atttgctgat
    10861 ttgagcggtt cggccctgac ccccagcctt gtggaagttg ccctttcaga tcttttgccc
    10921 aaccgcagcg accttgaacc tgaaaagatc gtcacacagg ttgctgatta ttacaaactg
    10981 tctgtggaaa agctcatggg acgtgaccgt accaaagacg tcgcctactc ccgccagatc
    11041 gccatgtacc tgttgcgtga ggtttccaag atctcattcc cgcaaattgg cgaagcactc
    11101 ggcggccgcg accattcaac ggtcatgtct gccattgaga agataaaaga ccaatataag
    11161 agcggtgatg gcagggtaaa gaaagatgtg gatttcctga cgcagaagtt atacgcctga
    11221 aattgattgt agagcgagat gaaatctcgc tctatttttt atatgggaac tggtgtttga
    11281 ccatagccag caagataaac gccccgccag cggcaaagaa caaatacgat tgtagaatgt
    11341 tgaggtagga atccgcaacg aatttggtga atggaatgac atttgccgcc agcaggtagc
    11401 aagttacaaa cgcaattttt acccaaaggc ttttcccgaa actttctcca acccaaatga
    11461 acaaccccaa acatacgaaa atagggaaga ggatgccgta atgatgctcc caggcaatgg
    11521 gagatgccag cgtagcggcc agacccatca ggaggaaatc tgcgcttcgc gcctccgtgg
    11581 tgctgctctt tgttttccat aaaccgatca gcaggatggc aatggatgtg acctgggtaa
    11641 acgcaaagat ccatgtgttg taaggcggga agtaatatcc gcgccatttg gtattgttga
    11701 acacttcagg gtatttcacc ccgaaaattc tgcccgccaa tccattgaaa gattggttgg
    11761 agtagtagga ttcaccatgc tgagcaagga aactcagccc gcggaaatag tccacgtaca
    11821 tggcgaggcc gaaaacacgg accccgagca gaagccccac agcccctgtg atgatcatgg
    11881 caatcaccaa tcgtttgttg ccacgcagca gaccccagaa aatgaacagc gcatactgcg
    11941 gcttgatcga tgccatcaat cccagcagaa tacctgccag ggtctcatat cctgtgaaat
    12001 agcacaggat ggccagcgca aaaaaggcat tcaaccatac ctgtatttgt ccgagtgtgc
    12061 ctgctttgac ggcagggtaa aaagtaagca gcagtaatat gagcaaagcc ccgaaaccaa
    12121 ccttttcggc cgcgttgagt tttggtgcgt cggtttttcc gtatgagtaa aggctggttg
    12181 cgatgacact ggccgccatt agtagaagga acaggaaggt ggtggcagtt gaaaaggcga
    12241 gcaggttgat ctggttgttc tcgataaaac gggtgatcaa cagcgcggtg ggtgggtatt
    12301 gaaatttaac cttgctggtc agcagtaact cgctgtaaac cagactttgt ccctgagatt
    12361 ctttccaatg gtcaaaggcc attttcatcg gtcgccagga atcgtccccg atttcgcggg
    12421 tgaagatcca gatggcgtga tccactgcca ttttgttgtt tttcaaacca agggaatttg
    12481 tgatcccctg aattgcaaag gaaaaaacaa ggttgaggac aaagagaaaa aggagttgtt
    12541 tgaggatggg cttcatggtt ttccgcgtaa ttttttagcc tagtatagca ttttcctatg
    12601 gtataatcgc gccgccctga aatcttcagg gcaacattct gcctgggacg ggcaaaagcg
    12661 tccctgagcg ggtctggctg atcaggccgt atccccgcta aactcattct gttccatgcc
    12721 agattgcatc tgcgcacagg gaacagcaca agttaaggag taacaaatgg ctgtcatttc
    12781 catgaaagcc ctgcttgaga gcggcgtcca ctttgggcat cgcaccaaca agtgggaccc
    12841 ccgtatgaaa ccgtacatct tcaccgagcg taacggtatt catatcattg acctgcaaca
    12901 gactgtcaag ctgttgaacc agggttacaa cgttattcgc gataccgttg cgaacggcgg
    12961 tacggtgatg tttgtcggca ccaagcgcca ggcgcaggaa actgtggctg aagaagccat
    13021 ccgctgcggt atgccctatg tgaccgaacg ttggatgggc ggtatgctca ccaactggag
    13081 caccatgtac cagcgcattc aggaattgga gcgccttgag aaggtgcgcg attctgatga
    13141 aatcaaccgc ctgaccaaga aggaaggttt gttgatcgag cgcgagatca cccgcctttc
    13201 cacccgttta tctggtgccc gctctttgaa gcgccgccct gacctgctct tcatcattga
    13261 tgtcggtcgt gaagaagctg ccgtacgtga agccaacctg ttgaagatcc cgatcgttgc
    13321 cttggtggat accaactgca atccgcagaa tgtggattat gtcatcccgt ccaatgacga
    13381 tgccatccgc gccatcaaac tgctggttgc caaagttgct gatgctgtgc tcgaaggcaa
    13441 ggccatgcgc aaggaagaag agcccgaaca gcctgcttcc gctgacgccc agtctgagaa
    13501 gcccaagacc cgtggtccac gcaagcctgt ggttgacgta gataccgaag aagtaggtga
    13561 ggattccctg cttggcgcct ccacactggc gaagttgaat gtttcccgca agggtgatga
    13621 tgttgccgcc gatgatacgg ccgcggctga ataatcaagc gcaagaaaaa aagaaagtct
    13681 aaggatagtg aaatggaaat aacaacacag atgatcaagg acctgcgtgc tgccaccagc
    13741 gcccccatgc tggattgccg caaagccctt caggaagcaa acggtgattt tcaaaaggcg
    13801 gtggattggc tgcgcgaaaa aggcatggct actgctgcca agcgttccga ccgcgtcgcc
    13861 tcgaatggca ttgttgaaat gtattcccac ggcggtggtc gtgttggtgt gatggtggaa
    13921 gtgaactgcg aaaccgattt cgtcgcccgc gccgaacagt tccgcagcct cgcccatgaa
    13981 atcgccctgc aaatcgctgc aagcgccccg aaatacatca ctgctgaaga tatccctgcc
    14041 gctgaacttg cgcatgaagc cgacattgcc cgcgcccgtg ccattgaaga aggcaagccc
    14101 gagaagatgc tcgacaagat cgtggaaggc cgcgtggaaa aatacaaaga tgaagtttgt
    14161 cttctgcgcc agacctacat ccgcgatgag tctctcacca ttgaaaagct tattttgcag
    14221 aacgtcgctg ccattggtga aagtgtgatc gtacgccgct tccagcgttg ggagctgggt
    14281 gaaagcgcag caagtaatta gttttaaaaa gccaaccttc gggttggctt tttttataat
    14341 ttataactga aaatacacgg aggtagaaat ggcagcattg aaatacaaac gcatcctgct
    14401 caagttgagc ggggaggcac tcggcggaaa atcaggtttt ggtattgatg tggatgaagc
    14461 agaatcgatc gccagtcgaa tcaaggaagt tcacgagatg ggagtggatg tggcggttgt
    14521 gatcggtgct ggaaaccttt ggcgtggcaa acaaggcctg gagcgcggca tggatcgttc
    14581 cacagccgat tacatgggca tgcttgccac tgtgatgaat gccatgtcgc taatggatgc
    14641 cctggagcgg cagaatgtat ttacacgggt gatgtctgcc attgaaatgc gcgccattgc
    14701 cgagccatac atccgccgcc gtgcagtacg tcaccttgag aagagacgcg tggtcatttt
    14761 cggcgccggg acggggaacc cgttcttctc cacagatact gccgccgccc tgcgtgcaac
    14821 cgaaatagac gcagatgtgg ttatcaaagc cactaaagtg gatggcgtgt acgatgcgga
    14881 ccccaagaag aatccagatg cgaaaaaatt tgaccagcta agttacatcg aagtgctgaa
    14941 tcgccgtctt gaagtcatgg atagcactgc cattaccttg tgcatggaaa ataacctgcc
    15001 cattcttgtg ttgaacctat gggatacaaa ggcattactc ggcgcattgc gtggtgaaaa
    15061 agtagggaca ctggtcagtt cctaaagatc ttgctgtttc atgaaatgaa atccgcattt
    15121 cctccttcca ttgcgaagga ggaaattttt taaatacatg agagcctctc aaatttttgc
    15181 atttcctatc gcccttttat cactcccata gcataaatcg ttgttttaag gtatccttgt
    15241 agcgtaatta ttttcaggag gaagatagtg agcaccgacg aaattagaga cctgttgaaa
    15301 gatgctgaaa cccgtatgag cggagcaatc cacgccctta ccgatgacct ttctggaatt
    15361 cgcaccgggc gtgcaagccc tgcactggta gaaagacttt ctgtggaata ttacggaacc
    15421 cccacgcatc ttatgcagct tgcaacgatc agcgttcctg aaccgcgtac tttgatgatc
    15481 aagcctttcg atgcgggatc ggtcaaggat attgagcgcg cgattcgtgc ctcaaacctg
    15541 ggcctgaatc ccaattccga tggaaaggtc attcacctga acctgcctcc gcttgatgaa
    15601 gaacgccgcc gcgaccttgt gaagcagatg agtcaccgcc ttgaagaggc ccgtgtggca
    15661 gtccgtaaca ttcgccgcga cttgcataac gacttgcgcg attatgaaaa agaaaaactc
    15721 atcaccgaag atgacctcaa acgcggcgaa gaagacttgc agaaactgac cgacaaattt
    15781 attgaagata tcgccagaca cggcggcaac aaagaaaaag agatcatgga agtgtagcat
    15841 cttttgagat gcccctttgg catcaagaag agtgtttcaa acatggctga gatcccatct
    15901 gcgattcccc ttgaaaaaat tccccaacac gttgccatga tcatggacgg caacgggcgc
    15961 tgggcacttc aacgcggctt gccgcgtctg gcaggtcaca aagcggggac agaaaatctg
    16021 cgccgtgtca tacgtgcaag cgtggaattt ggcgttaagt acctgaccat ctacgctttt
    16081 tccaccgaaa actggggcag gccgcccgaa gaagtaaaag ggttgatgta tatccttgaa
    16141 gatgtcattg accgcgaatt gaatgaatta aacaaggaag gtgttcagct gcgccatatc
    16201 gggcgcctgg aaaggctggc accctccttg caggaaaaag tgctggatgc gattgaagcg
    16261 acaaagaaca ataaccgact ggttttaaat gttgccttca attacggtgg acgggatgag
    16321 atcgtccttg ccattcaaaa aatgatgaag gatggcatcg ccccggagca ggtgaccgat
    16381 gaattggtca gtcagtatct tttcactgca ggtgtacccg acccagacct gatcatccgc
    16441 acttcgggtg aattgcgcat cagcaacttt ctgatctggc aggcggcgta cgccgagtgg
    16501 tatatcaccc ccaccttctg gcccgatttt gacagggaag aatatcatcg cgcgttggaa
    16561 gcctttgccc accgcgatag gcgttatgga aaggtatctt cgggggaact tcaggaatca
    16621 aatgcgtaga agactaatta ctgctcttgg attggctgta ataggagtgc ctgccattct
    16681 attgggaggc atcttttatt acctgttgat gggaaccttt ttgatcggtg cggcatggga
    16741 atatgtgcat atgttccgtg ccgtgaagtt cgaacctcaa atgcatttga ccgtgggcgg
    16801 cgtggctctg gtgacactgg cacgcatgtt cgctcctgaa ttcgcaatgc ctgtttttgg
    16861 cgcagccatc cttgcagcat tgacctatca tctcatcgct tatgagcgcg gccgtgatca
    16921 ggccgcgctc gatttttgca tctccatcgg cggtgtggtg tacattggct ggatcggttc
    16981 ctatctgttt gacctgcgga atcttccgca aggcggctgg tggttcatgc tggtgatgtt
    17041 ctgcgtgtgg gcgggcgatt cgggggcata ttccatcggc agggcttacg gaaaacataa
    17101 gatggccccg cgcctgagcc ccaagaaaag ctgggaaggg tatgttgcca gtgtttttac
    17161 gggcatcatc actggtggtt tttatgtatg ggtctttcaa aggtttggca gcctgacgca
    17221 cgatatcagc atctggcagg gggcgatcct gggtttgttg ttgggcgcct tccccacgtt
    17281 gggtgatttg ggcgagagca tgatcaaacg acagtcgggg ttgaaggatt cgagcgatat
    17341 catccccggg cacggtggtt tttttgaccg catcgactcc tggttgtggg gttcgatgat
    17401 cggtttttac ttcctggttt ggttcatcct gtagagattt ggaggcaaaa atggaagttc
    17461 gcggcgctac acctactcgt aatcttgcat tggaactcgc acgtgttacg gaagcagcgg
    17521 caatgttggc aggtcgttat atggggcgag gagataaaga aggcgctgat caggctgcgg
    17581 ttaatgcgat gcggcttgtc ctcagtacag tggatatgaa cggggtcatt gtcatcggtg
    17641 aaggggagaa ggataaagcc ccgatgctgt tcaacggtga gaaagtaggc aacggttcag
    17701 aacctgacgt ggatgtggct gtggacccaa ttgacgggac tcgccctttg gcatttggcc
    17761 gctcaaattc tctggccatg gtggcgcttg ccccgcgcgg aacaatgttt gaccctggcc
    17821 cattcctgta catgaataaa cttgcagttg gacctgaagc aaagggaaag atcgatatcg
    17881 aaaagcccat ctccgaaaac ctgaaagcca ttgcaaaggc caagggcaag cagatcgaag
    17941 atctgaccac gatcatcctg gatcgtcccc gccatgatga tatggtggct gaaattcgga
    18001 aagcgggtgc gcgcatccgt caaattcctg atggtgatgt ggccgcggca ttgatgacct
    18061 cctggcctga ttcaggtgtg gatgttttgc ttggtattgg cggtaccccc gaaggcgtga
    18121 tcaccgcctg tgccttgcgg gcaatgggcg gcgagattca aggcaaactt ttcgcgcgtg
    18181 acgaggatga attgcgccgc ggacgtgaag cgggttatga ctttgaaaaa gtgttgacga
    18241 tggatgacct ggtctcgtcg gaggatgtgt tcttcgccac gacaggcatt accgatggcg
    18301 aattgcttca gggcgtacga tattacggca atcacatcac cactgatacc cttgtggtgc
    18361 gcggtttgac aggcaccatt cgccgcatca gcgccacgca tagcactgat aaattggaca
    18421 acctgagttc ggtacattat taatttcact tgacattcca cggccttgtc cgtataatgt
    18481 cggtcgcttt cctgactagc tcaatcggca gagcgagcgg ctgttaaccg ctaggttcta
    18541 ggttcaagtc ctaggtcagg agccaacaaa gtccctcatc aattgaggga cttttacttt
    18601 aaaaaagtcc cctccgcagc ggtgagggcg ccgaggaggg gggagaagtg ggctttatga
    18661 actaatgctg acccaatgtc ccacgtgagc ggacgttgcg ctcgacccga taaaagaacc
    18721 atgagccgaa ccacaacatg aggaacatgg tgccgatgag cagggcaact tcaacgggta
    18781 acggcaggag cgtattggtt ttcagtatcc aaccgcggac ggcatcgaag ccgtaggtga
    18841 ggggcagcat caatgagatg gggatcagcc agtaggggag cgacttcacg gggaaatttg
    18901 cacctgtaag cccttgaaca agaaaactac ttacatccac catggtgttt gcgtcacgca
    18961 tcaccagcac gatggcggca aaggcgaagc caaaaccgta caggccgatc agcatgggaa
    19021 ttgcggtcag aatggcggcg atgatgttgc ctgtgggttt gaaaccgaag agaagcgcgc
    19081 caatgatcag catgaccgtg gaagtgatgg tcgtggtcaa taaactggtg agggtgcgcc
    19141 cgacaagaat gtgcagacgt gaaatgggag tcagccaatt ggattcgagc acgccagcat
    19201 ccatgtcttc tttcagggca taccccatgc cccagaacac cgtcatgatg aaattgctca
    19261 gtgcggtgcc gatcaaaata taagacatgt aatcgccgct gccagtgtag ccagcgaatc
    19321 ccagcgcctg tccatttgtg ctgaaggctt tgcccatgaa ataaacaggc gtgacccaga
    19381 tcaacggttg aaagatgctc gaaacggcgt tcaacggata acgccagaag accttccagt
    19441 ctttgcgggc gatgacgtac aaggcgcgga gttgtgtttg ccagtttgtt tgcgagaggg
    19501 tggtgatttc cataatatcc tcttagtatt gcccgattgc accagtgatt cttgcgcggc
    19561 gttccatcca cacaaaaaga gagtatccaa gggccagcca gaacagaccg aataacagaa
    19621 tgggaatcag gtcgggggcg agttcgggaa tgcctgcatt gttgaacgct gcggcgcgca
    19681 tcccgtggat gagataggtc tgcggcatga atttggcgat gccttgcatc cagccaggca
    19741 gcaagctgac agggaaggtg attccgcaga agatcatcac caatccacgg acgaggaata
    19801 caaatgcatt cgcttccttc atggtgatga cgacactcgc aaaggcgaag cccaagccgt
    19861 aaatggcggg gatcgcagcg agcatcatca agccgatcat ccacgggttg ccgttcagcc
    19921 gcacaccaaa gaacacgcca aactcaaaag cagtgatcga gacgaacagg agcatggaaa
    19981 ccaattgcgg ccccgtgtgt ccaagcaggt aggcaaagcg ccaggtaggg gagagccagt
    20041 tcgattcgag cgtgccgcgc atctgttcct gccgcagcgc gaagcctaca tcccaaagta
    20101 cgatgttttg ccacatccat accgtggtgc cgacgacgat gtagccgatg tactcagttg
    20161 cgcctgtggt gttgaggaag acctgcaatc cgctgccatc ggggccagag agagcgtgcg
    20221 ccgtcaggat atacatcatc gggaagatca gaggccagat gaacatggcg atgatccatg
    20281 aggggtaacg catggtgatc gtccactcgc ggcggacaac cgcccaaaga gcgcgcaggt
    20341 cagatgcgaa gccaggcctg ccgagagatt gaggggaaag ggttgtttgc atatcagtct
    20401 cgcagtgcct tgccagtgag gtgaatgaac acatcttcca gtgaaggctt gacgatattc
    20461 atattgacaa gtcttgtgcc gttgccgctc agtgattcca ccacgcgagg cagggcggtg
    20521 cggctgtttt gggcttgcat attcacttcc catagatccg cttcgccttg tttgcgggtg
    20581 gtgagcgttt cgattcctgc aatgcctttc aacttatcgc tcaggtcatc gtgccagcct
    20641 gtcaattcga gtttgatcac ttcgcgctga tcgatcttgc gtttgagtcc ttcgggcgta
    20701 tcgagcgcga tgaccttgcc tgtatcaatg atgccgatgc ggtcggagag ttgatcggcc
    20761 tcttccatgt agtgtgtggt cagcaggatg gtatgtcctt ccgctttcaa agtggcgatc
    20821 aactctctta agttgcgagc cgcttgcggg tccagcccga gcgtgggttc atccaaaaga
    20881 agaatgggtg ggcgggcaag cagcgctttg gcgatcgcga tccgttggcg cattcctgtc
    20941 gaatattttt caaccaattc attggcgcgg tctttgatct ccatgcgttc gagcaggtcg
    21001 tccacgcgtt tctttgtgat cgcggcgggg atgtgataca gcgccgcaaa atattcaagg
    21061 ttctcgcgcc cggtcagctt ccaataaatg ctgcgctcgc cagccagcac cgtgccaaga
    21121 ctgcgcctca catcgtttgc ctgcgcaacc acatcaaagc cgttgacctt tgcagtgccg
    21181 ctggtgggtt cgagcaaggt acacaacatg cggatggtgg tggatttgcc cgcgccgttc
    21241 ggccccagca agccaaaaat ttcaccgcgg cggatttgca gatcgactcc gttgacggcg
    21301 gtgaacatgg cctgcggctc tttcttccaa aattgccagc gcttcttttt tgaaccttga
    21361 gcggattcag tctcgacagc tttatctccc gtccctgggc gggcggggaa cttcttgacg
    21421 agttgggtgg cttctatggc gaaatcagac atgggggact cctttttgtt gaacttcaag
    21481 aaaatgaagg tgacaattcg taatattgac ttttttgttt gcttattttc aatattttac
    21541 gaaaatactt tgattttgtc aatgccgtca tttataaaat caaaggtagg ctcaatttta
    21601 ttgaacacat tgtctgtagg gttagacatc tcacgctgcg cccccacatc taggtgacaa
    21661 atctctgcct tgcgaataaa ataccaatcc atgtggggac gactgattcg cgacccgaag
    21721 ggagattttg atgaaaattc tcaaagaaag aaaaggccta ttcgtaaaaa tactggtcag
    21781 tgtcttattt gtattcctgt gttatggatt gtcggaaaag gcgttccatt gggcggtgtg
    21841 cggagaaaaa gagcgcaaag aattagatat gttgttccta atgttttgcg aaaaagaagc
    21901 agatgcatgg tttgaaaaac ggttttggga ggaatgcacc aaaaatgaca agaacctgcc
    21961 gcctcttaca gaacttttga tttctgcttg caaagaacct gctgtgcgtg gtttttccga
    22021 tggagaaaaa ttatttgtga ttgagggtaa aactgacgaa gtttatttct tggatttgcg
    22081 cacgggagaa agggcacccg tcccagatgg taaacaattg tgggatgagg ttttcctgag
    22141 tccggatttg gtctggtttg atggatattt aaagcctggt gatactgggt acatacccca
    22201 ttacgttttg gatttgacag acggaaagaa gtacgaactt tttgatctat cctggatgcc
    22261 gttaaaggat ggcaaatttg atacccaaaa ctatggatat attaaatctg ccgaacatgt
    22321 ttatttttcg ccgaaagaaa atgctgtaat tgccttggcg cctaacttta ggcaaagccc
    22381 cagacaaaac gttatatatt ttgactcatc ccttgagaaa ttattgaacg atctgggggt
    22441 aaattatgaa ctcgtggatt actcgcttgc tgacgcagat gtcccatctc caacagggaa
    22501 gtatgtggtt cgtaacgatg ggaacgggat atatttttcg ggaactaatc tccccataat
    22561 aactggtgcg tatagtagca gttgtttcaa aagttggtat tacgacgaaa gcgctgtggt
    22621 cattgcctgt ggcatttcgg gctatctata tcataattct attacacata gcgtaagtca
    22681 ccccattcct ctccccgttc ttaaactgtt tttgcctact ccatgagaag aatcattacc
    22741 cgccatgcgt agcgcgggat attatcccgc gctacggttt gttgcgttaa tttcttctct
    22801 acggccacgg aatttcagcg gggcctgcga agccgtgttt ctcctgaaag aacaccctgc
    22861 catgtgacgg ttcggcgcac ccgccttcat ccacaaaaga gacttcgggg taattggatg
    22921 ccagtgcttc gaagacgatc tgtccgccct cacagcgcca tacttcgatg atgtaaatga
    22981 acatatcggc atcttcttca tcaccagcgc ggagttcgac atcggtccat ttcacggtga
    23041 cagtgccgcc gttattctcg gcgctcaaca cggctggggg agcataataa ggcgactcag
    23101 ggagttcata ttcgcccggg taggtcacac gcaaagtttg cggatcacct tccacactga
    23161 tgaatttttg gttgacccag cacttgtgct ccagtccgtc gaccatcacc cagttgctgg
    23221 catgggcgcg accgatcaag gtaagtttga aatcttttct gaaaccataa aagtaaagat
    23281 attccgcgcc ggggccgtag cggcaactta acgtgtcggc tgtgacaacg gccttcaaac
    23341 tatccacagt gggaatctct gtgggcgtgc tggttgacga agccgtgggc gggggcaggg
    23401 ttggcgtgaa tgtttcagtg ggtgggatgg gggtgggcgg aatggcggtt tccgtcggtg
    23461 cggcgacttc gagcgccgtg gctgtcgctg cttccacggc aggtgcggct tcggcggggg
    23521 cattcgtcgg cgcagaggaa ggcacattac atgccgccag aatcaggacc agggttaaag
    23581 taagaacggg gtgtattttc atgatcaagt ctttttccat tccataaatt tgatttcttc
    23641 aacatcgctg gtgggcagca atgtgatttt atccttacgc ccttcacgga tgtaaatttt
    23701 gtaaactgcg tctgctgggg tgcggacctt tgccacaaga ttgacctttt tatttacaac
    23761 tgcaagcagg tcgagccgtt tgacaaaaag aaaggatgat tccttttcga atgcgatcaa
    23821 atctgccttg ccgaataacc agccaacctt gccgcgcaca ttgcgaagtt ccacccaggt
    23881 gaaatcggtc tgttcggttt tatcgcccct gcggattcgt ttctggcttt tcacttccac
    23941 tttgaaaacc tcgcctgctt tttcgatcag gtaatcccag tggtcgtcaa tgttctcatc
    24001 cttcgatgag gccgaaacct tccagcctga tctgaccgcc agcacgatga aaagattttc
    24061 agcgtgttcg ccaagttcca gcgaatcttt tgtgtcgaat ttgttgcgag agttggccat
    24121 gctatttttt ctttttgggt tttacaggtg cgggcgtaat gcccaggatt cttttgcgcg
    24181 ccagttcgca gtatttcctg tccacgtcaa tgccgatgcc tttgcggttg ttattcaccg
    24241 ctgccagcag ggtggtgccg cttccgctga acgggtcgag cactacatca tccacataac
    24301 tgaaaagctt gatgcagcgg cgcggcaatt caagcggaaa aggggcgggg tggccgattc
    24361 tttttttgct ttcgccgttg aaggtccaca ggccgttcgt ccaatccatg aactcgtttc
    24421 gttcaatgtc tgaaattttg ctgccgctgg tcttcttcca attgtttttg tacagcacga
    24481 tgatcaattc cacaggagcg atcacgtacg gtgcggatgc acttttccat gacccccagg
    24541 cggttctgcg cgaaatgttg ccctcgttcc aaatgatggt cgagtggtag ttgaacccga
    24601 tctgttttgc gatggtcgtt agatctgcgc ccacactttg ctgcccgcct ttgtttttat
    24661 cgagcggcac attcaggcag aagcggccat cgtctttgag ccagtcaaaa cagcgcgaca
    24721 tccatttttt gctgaactcc aaataatccg catacgaaac gtcgtccttg tgcgaattgt
    24781 attggatgtc cacattgtaa ggcggggagg taacaacaag gtccacgctg tttgcgctca
    24841 cgagcgatgt ggtcaaaata tcgtcgtgaa cgagcgtgaa cctctcgttt tgaaaatgga
    24901 ttccgaatgc tttcatgctc ggattatagc cgacgcctta tccgctttgt gaatcaaaat
    24961 tgccttccaa aatcttttcg atgaattgtt tgaaaacgga aaagggttgc gcgccttcga
    25021 ggatgtgacc gttgatcaga aaagtcggcg tcgcgtggac tccatccgct tcagcctgcg
    25081 ccttgtcctg ctccaccgtc cctttgtgag ttccctcgct gagacaggtc tcgaacgcat
    25141 ccacatccag gttgaccgct ttggcatatt tgatcagatt ttcctgcgta aaatcgccta
    25201 cgttttcacc tgtccagttg gcatagagcg tgtcgtggta ctcccaaaac atgccctgat
    25261 cccccgcaca gtaggcgcct tccgctgcca aaacagattc aggcccaagg aacgggaatg
    25321 agcggtattc aaaatagacc ttgcccgtgt tgacatactc ctcgatcaaa agcggctcgg
    25381 tctcgttcca gaacctgagg cagtatgggc actgaaaatc gccatattca atgatgtgga
    25441 tgggcgcatg cggatcgccc atcgtattgg cctgtatcac tgcacgtgga agttcctccg
    25501 ccacttccac cttgtcagga aagaactgct ggacaaattt tggccccaca aagatcagtg
    25561 tgcctacaaa gaccgccgcg caaagcagca ctgcgcatcc gcctgccgca agccataccc
    25621 gccagttcgt tttttgcggc tcaacgatca caggttcatt ttgtgtttcg ggtgtgggct
    25681 ggttttcaag tgggggttgt tcttcgatca tgattcttcc tccgggcggt atctttttcc
    25741 gttgcttttt ctctgtgtca tctgtggtgg atgttttttt tgtcgtttcg ccactccctt
    25801 tttggagcgg acattcaata ttccctcaga cgctgaatgg gggaattcga ttacctcacc
    25861 acaacttcct tatctaaaac tcatacccac atcataaacc gaaatcattt tttcgatata
    25921 atttcaccat gaatgccacc cttacctcca accagaattt acccaaacag atcgttgccg
    25981 cacttgtcgg cggattctcg cttttcctta ccgttaccat cacatggacc ctgggctatc
    26041 aattattgta tgcgggccgc attttccccg gtgtttctgt ggcaggcgtg gatgtctccg
    26101 ggctgtccat caatgatgct gcgttgaaat tgagccagac cctttccttc ccgaatacgg
    26161 gcaagattct gtttcgagca ggcgataagc tttgggttgc atcccctgca gaattgggaa
    26221 tggtgttcga ccccacagcc agtgctctgg ccgcctatcg ctatggacgt tccggcggct
    26281 tgtttggtgc gttatcgggt caggtcagcg cccgtggact tggctcagat gttgccccgg
    26341 tcattatttt cgaccaacgg gtcgcctaca cctatttgca aaatattggg gcacaggtta
    26401 accaacctgt agtggaggcc agcctgcacc ttgaagggac gaatgtggtg gcccagcccg
    26461 gacaggtggg cagagtgctc aaccttgatg ccactctcat ctacctcggc gctcaattgc
    26521 aaaccttccg cgatggcgag gtgccgctgg tgattcagga agccgcgccg aaactgttgg
    26581 atgtctcttc acaagccgaa gccgcgcgcc gcattctcag ccagccattg accctcagcc
    26641 tgcccaatta tcgcagcggt gaccctggtc cgtggactta tgatgtgccc gtggttgcaa
    26701 acatgatcgc ggtgatcgtt gtcgaaaatg gcggcggtgc cgaaatgcaa gttgggttgg
    26761 atccgaacat tttgcggcaa tcattgaacg aactcaaaac ttacgtagac cgccagccca
    26821 cgaacgcgaa attcgtcttc aacgatggca ccagccaact tgaacccatt gcggcctcca
    26881 atgtcggccg cgccatggat gtggaagcca gcattgccgc cattaacgat gcgttgttgc
    26941 gcggcgaaca caacgtatcc ctcgtcgttg ctgaacagca gcctttggtg gcagatacag
    27001 ctgtcggcgc agatttggga atcgtgcagc tcatcgccga acaaaccacc tatttctacg
    27061 gttccagcac cgaacgccgt cagaatatcg aagcggccgc cagtcgtttt catggtgtgt
    27121 tggtggcccc cggcgaaacc ttctcaatgg gcagtgtgat gggcgatgtc agcctcgaaa
    27181 atggatttgc cgaagcgctc atcatctatg gcgggcgcac catcaagggg gtgggcgggg
    27241 gagtgtgtca ggtcagcacc acgttgttcc gcactgtgtt ttttgcgggc tttcctgtgg
    27301 tggagcgtta ttcgcatgcc tatcgcgttt cttattatga aaagacctcc agtggctctg
    27361 tagactcaaa ccttgcaggc atggatgcca cggtgtactt cccgttggtt gatttcaaat
    27421 tccaaaacga caccccgtat tggattttga tggaaaccta catgtctgaa aacagcctaa
    27481 cctggaaact ctattccacc tccgatggac gcagtatcac atgggagacc acaggcccga
    27541 ccaatattgt ccctgcgccg ccgccgttgc tcgaagaaaa ccctgaaatg aaagccgagc
    27601 agatcaaaca ggtggattac gccgcacagg gcgccgatgt caatgtcacc cgcgtggtct
    27661 ggcgcaatgg agtgatctat ttcaccgacc aatttatcac ccattaccaa ccatggcagg
    27721 cgatttgtca gtatgggcca ggcaccgaag acccgaaaaa actggcaagg cgaaacgaca
    27781 tttgcgttgg agccagttag ctgaacaaaa tgattttgat tgcaaggctt tgtggtaata
    27841 aaacggactt ttacaacgga tctggagtcc aagctcacgg gcttttcaag atttaagaag
    27901 aaaagcggga aaaacccatc atattcccgc atggtacaat ccttccctga aaaggagaat
    27961 acatggtcag ctcagaactg ctcgaaattt tacgatgccc caattgcgtg cgcgaaaaaa
    28021 gcggcacgct ggttttgcac aaagatgcct ggcttatctg ccaggattgc ggtcgcaaat
    28081 accccattgt ggatgacatc cccgtgatgt tgattgacga aggcgataag tgggtcaaca
    28141 ccgcacaaga cgccctcccc gtccccccgc ctgaaaaata aaggttcgct tgagcgaaac
    28201 taaaatgcag gaactgatcg ccgagcttct cagcggcgat gatgaacgcg ccgaaaaaac
    28261 catccctgcc cttgtggggt ttggaatggc ggcgcattct gcattattag acctgacccg
    28321 tgccgcagat gtggattcgc gctggtgggg ggtacgtgcc ctcgccgcct cgcctcacac
    28381 acagaccgaa gacctgcttc cgcttctcag cgactcagcg ccggaagtgc gtgccgccac
    28441 cgcccttgcc ctgtgcaatc atccgcacga ggcggctgtg gaggcgctgg ttcaagccct
    28501 tgcggacgaa gacccgctca ccgcagggtt ggcagggaca gccctcggca agatcggtgc
    28561 tccttcggtg ccgagcctgc tcaaggtgat gagcgaagcg ccggttggcg tcaaaattct
    28621 ggcgttgcgg gcattatccg aaattaaaga ccatcgcgcc atccccgtca tgatgaaatc
    28681 aatgggggaa gaatctgcgc ttttgcaata ttgggcgcag gaaggcttgg aacgacttgg
    28741 gctaaatatg gtatatgtta agccctgagg tacagaaaac aatgaatcga tttgaattcc
    28801 tgaagaaaat aaaactgccc aaaccaaaaa tcccctcggt gggacgcatc atcttttggg
    28861 ccatcagcat tgggttggcg gcagcgacat ttgtctttgc acgcaatttc acctcctgct
    28921 ggaacatcac cggcctgcct ggcattgctc cctccagttg cggcacatca accaacccgc
    28981 tggctggccc ggatttgacg gcagccaccc aggaacccgg tgcagtggat acactgccca
    29041 ccccggcccc cgtggctccc ggtgtggaat tgccccccgc atgggatggt ggcagccgta
    29101 tcaacatcct cttcattggg ttggatttcc gcgattggca ggccaacgat ggaccgccgc
    29161 gctctgatac gatgatcctc ttcaccattg acccggtcac aaaatcggcg ggcatgttgt
    29221 ccattccgcg cgatttgtgg gtgaacattc cagaagggtt tggatacagc cgcattaata
    29281 cagcatactc cagcggtgaa ggcgcaaaat tgcccggtgg cggccctggc cttgccatga
    29341 aaaccgtaga gcagttgctg ggtgttcctg ttcattacta tgcccaggtg gatttcaaca
    29401 ctttcactga attggtgaat cgacttggct gtatttatgt ggttccagag gaaattcttg
    29461 tgcttgaccc tgtgggtggt ggcctagatc atgtggtcat cacccccggc ggcgaacgca
    29521 aactgtgcgg ctggaaagta ttggcctatg cccgcacccg taaaaccgaa ggcggcgata
    29581 tggaccgtgc ccgccgccag cagcaggtga tcttcgctct tcagcaaaag atcttcgacc
    29641 ctgaggtttt cccctctttg atcgcacagg ctcctgaaat gtacgcggaa ctctccagcg
    29701 gtatccacac caacatgcca ttggatacgg ccatcaaact ggctattttg ggcaaagaca
    29761 tcactcgtga aagcatcaag accggcatca tcggcaccga tatggtgacc tttgaaagca
    29821 ccaccctcgg tggacaggct gccagcgtca tgaagccgat catggataag gtccgcgtgt
    29881 tgcgtgatga gatcttcacc agcggcggcc ccaccagccc aatggctcaa ggtgaacctg
    29941 ctgccctgat gcgcgaagaa gaagcccgcg tccgcgtggt ggatgggacc ttcaccactg
    30001 gtctcgacca gcgtgcaggc tcattcttcc aatctcaagg tatgaatgtg accgaggttg
    30061 gccccgccag cgaaatatat tcctcgacct tcatcattgt gtatggcccc aaactgtaca
    30121 ctgtgaaata cctgcaagcg gtctttggca tccccgcctc gaacatccgc ttcagccccg
    30181 accccgcttc aacggttgat atcgagatcc gtctcggttc ggatctggcg ggaagtattc
    30241 cctagccttt tgaaaagcaa aacatcccat cttctgaaaa aagatgggat gtttttttat
    30301 cgttccatca cccctgaaat gggaaaacaa aaagacccgc ggaat
//
LOCUS       DAOQLC010000045        29826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_494_length_29826_cov_23.482181, whole genome shotgun sequence.
ACCESSION   DAOQLC010000045 DAOQLC010000000
VERSION     DAOQLC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_494_length_29826_cov_23.482181"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            486..3173
                     /locus_tag="PL000_09800"
     CDS             486..3173
                     /locus_tag="PL000_09800"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNC89236.1"
                     /translation="MTRQELPKAYDFKSTEPRIYAMWETGGFFKPHNDPNKPGFDPSV
                     KPFVISIPPPNVTGELHIGHAMFVSVEDLMIRYHRMKGYSTLWVPGTDHAGIATQLMV
                     ERDLLKNEEVTREELGREKFLERTWEWKKKYGGMITNQIRRLGASCDWDRERFTLDEG
                     LSRAVREAFVRLYEKGLIYRGPRLINWSPGLKTAVSDLEVEYTEENANLYYFKYMIAG
                     SDEFIPVATIRPETILGDTAVAVHPEDDRYRKFIGKKAIVPILGREIPVIADEYVSME
                     FGTGALKITPGHDPNDYAIAQRHNLPIISMLDKAAKINENGGQYKGQDRFEARKNLWA
                     DMKAADLVIKTEPYRTTIPRSQRGGEIVEPMISEQWFVKIESLAQAGLDVVRDGRIRI
                     VPERFEKTYFNWLENIKDWCISRQLWWGHRIPVWYCPENHMTVAREDPTQCATCGSKD
                     IRQDEDVLDTWFSSGLWPFSTLGWPEETPDLKYFYPTSYMETGYDILFFWVARMIMSG
                     LEYTGQAPFHTVYLHGLVRDEHGHKMSKTKGNVIDPLIIMDDLGTDALRFTLLVGSTP
                     GNDTNVGVKKVEANRNFANKIWNAGRFVISAIDGLQPGSELGEYTLADSWIWARLQGL
                     VRDVERQFQNFQYGQAGQQIYDFIWSDFADWYVEIAKQELAEGGARAARAADTLARVF
                     DMALRLLHPFTPFVTEEIWGHLHSALRESPLNEICKDWPDALIVAKFPEPRDPEGWEE
                     SKLAEFALIQEIVRSIRNLRAEKGVSPSKKFAASISAGAKADLLKEQAQVLASLAALD
                     SAQLTISEALTEKPKDAIALVVGSVEIYIPLAGMVDLANEKARLEKELKEAESHIQRL
                     ENLLGGDFANKAPAALVAKEREKLNAYKDTAEKIKAQLK"
     gene            3278..3541
                     /locus_tag="PL000_09805"
     CDS             3278..3541
                     /locus_tag="PL000_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89237.1"
                     /translation="MQNPVQYAACPKCGQSNAKKVSYTFWGGALGPSLMTHVKCQSCG
                     TEYNGKTGQSNQKNIIIYILASFVISFCVCGGLAVLNFALNSN"
     gene            3753..3947
                     /locus_tag="PL000_09810"
     CDS             3753..3947
                     /locus_tag="PL000_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC89238.1"
                     /translation="MTMKIQDDCIACGACVPDCPTESISEGDGTLYVIDQTTCVECEG
                     HFDSPKCVSVCPVDCIVKAE"
     regulatory      3979..4141
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4318..5088
                     /locus_tag="PL000_09815"
     CDS             4318..5088
                     /locus_tag="PL000_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89239.1"
                     /translation="MTKLFNRIPWLGLFSLVAGLLAWDLTARLSGLPRFILPAPVDVW
                     RSFIKALTTGTLLYHTAVTLTEIVLGMLTGVTFATVVGYSLAKSRTLEKVLAPYLVAS
                     QAIPIVAIAPLLVIWLGDGILSKVIICALIVFFPVLVNTIVGVRAVPAALYDLMNSVR
                     ATRWQILFKLELPASLPILLGGLRVGATLSVIGAIVGELVDAQEGLGYLLQLGDFQYD
                     TSMVYVAVFVLIFLALGLYGIVTLLEKRFLKWQSTSSS"
     gene            5194..6174
                     /locus_tag="PL000_09820"
     CDS             5194..6174
                     /locus_tag="PL000_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89240.1"
                     /translation="MLKKLVYLMLGLAIGLSACTSSQGGSEAGTLRKIKLPMGYIPNI
                     QYAPFYVAVEKGYFAEEGIEIEFDYSFETDGVALVGAGELPFAVASGEQVLLARSQEL
                     PVVYAFAWYQQFPISIVSTPELNITTPADLKGQTIGLPGLFGANYIGLQAVLFEAGLT
                     PDDVKMDAIGFNQVEAFATKQKNVVVGYAANEPIVLRSQGMEVVELRVSDYVQLAANG
                     IITSEMAIKDEPDLVRGMARALAHGIADTIANPEEAYKTSEKFVENLAEQDKDLQMQI
                     LTTSIEFWKAEKIGYSDPSAWENMNDLLVKMGLIPELVDLTKAFTNEFVP"
     assembly_gap    6384..6483
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6485..7231
                     /locus_tag="PL000_09825"
     CDS             6485..7231
                     /locus_tag="PL000_09825"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89241.1"
                     /translation="MTIKPILTVKNISVVFPDDNGGLRALDDISFEVRPQEFICFLGP
                     SGSGKTTLLRILAGLLPPTAGRVNFIRNQHPEIGMVFQQSNLMPWRTVMDNIKLPLEL
                     EGMGETRARIKAREMIDLVGLNGFENSWPRELSGGMAQRVAIARALIHDPDLLLLDEP
                     FASLDALTRERMWMELSRIWQARQKTVIMVTHSINESLFLADRVLVLTQRPGKIKLDV
                     EVDLPRPRKDDIRYTPHFGKLARKLRETIE"
     gene            complement(7211..8536)
                     /locus_tag="PL000_09830"
     CDS             complement(7211..8536)
                     /locus_tag="PL000_09830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89242.1"
                     /translation="MLDVFRPIFSWDYTKKYWLAGVILIAMLCLSPIFHAYFNGNLIF
                     KSDGGAWAIASNLVGGKGYSGCATDYFPNCESTSQATAMREPIPVLLIALARLFHPTK
                     LSALIMQSLYYLGSIPAIYLTLKESSRNVKTALLGTLMWTFSVPVINQVDNGSGDLAA
                     AFFFSFGMYFFLRGYHASKAKDWVISGLFFGLAALSRTVLLGVGIGLGAVMLAKKWFE
                     TRPFSKKQIGGILLFLATLFLVFSPWVIRNTLVFGQPVIGSSLTGYNIYRMNFIVANA
                     DFQPHYVGAKEGYAAVRDLLSHSTLTGLENEAQLQSIYMKAGLQLITQHPIEYLQLVL
                     YRFLPLWFNVSVNEAYGAGYYMADYLSYVQQFLFLLAVLFGISKHWRSMWPFILALAL
                     GCGAYMAIDAQMRYLVDLMPAVVILSAAGLGSLMPQNEMKINRSLDGLA"
     gene            complement(8731..9210)
                     /locus_tag="PL000_09835"
     CDS             complement(8731..9210)
                     /locus_tag="PL000_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNC89243.1"
                     /translation="MFDHKEKQLADLETAYASFIDTVTGLASEAFFTTLGDWTPRDIA
                     AHFIGWNRITLAGCSALREGAEPFYFYDGTNDYRKVNAKFLSQFSATDRDTLLRDIAA
                     TKEILTAYLKTIPESEWEADTGIIHYRGGPATVARCVDSLIRDYRKHRQEIVDGIPI"
     gene            complement(9223..9642)
                     /locus_tag="PL000_09840"
     CDS             complement(9223..9642)
                     /locus_tag="PL000_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89244.1"
                     /translation="MNKPHPVYWVSAAITALCGIAVIGFGIQLVADGQDCKSYADLGC
                     AFRGVFGAVFIPYGALILLLAGLTSLPVRMIRLIGSVLSILAGVLHIGLCCFVASWAL
                     NGTSLKEVSGFLLVPFFIFASAIALLGMGFTGYKSLK"
     gene            complement(9639..10079)
                     /locus_tag="PL000_09845"
     CDS             complement(9639..10079)
                     /locus_tag="PL000_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89245.1"
                     /translation="MSLDPNYTQLNRASTERIKQLAASLSDEEMQTRVGEHWTVAIAL
                     AHLAWWDRRVMYVLDMTVKNGKLFVPEIDIIVNDLSLPLWAAIPPREAARIAIESSAD
                     LDAQLEAFPQDLLEEIYNYNKRWVVRALHRNEHLNEVDAALKAR"
     gene            complement(10089..10316)
                     /locus_tag="PL000_09850"
     CDS             complement(10089..10316)
                     /locus_tag="PL000_09850"
                     /inference="COORDINATES: protein motif:HMM:NF021552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HNC89246.1"
                     /translation="MCRSIKPLRNMDHPVTEQEIQEAALQYVRKVSGYRKPSKANEAA
                     FQKAVDEVAESTRRMLELLKNGSLQVKQFQE"
     gene            complement(10341..11126)
                     /locus_tag="PL000_09855"
     CDS             complement(10341..11126)
                     /locus_tag="PL000_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89247.1"
                     /translation="MNPQPKFPTLLHQKTAEFARDFFLNQSTVDTVLVVNSCARGQAT
                     PESDLDMAVLVKPETAHSDMQELETKWQAALQSNPVFQQFKVSHPFMQLHLDLITGQY
                     TPSIWDDGGGPDGFELEVGNQLAYGAPMHEIGSYYSQLQSAWLPYYDPPLQAQRLAMT
                     CHACTYDLDHVPFFFHRGLYFQAFDRLYKAFQEFLQALFISRKTYPIAYNKWIREQVE
                     QKLGLPELYRELPSIISVGNIESEEVIQKSLKLRTLLNKWIVQ"
     gene            complement(11127..>12164)
                     /gene="holB"
                     /locus_tag="PL000_09860"
     CDS             complement(11127..>12164)
                     /gene="holB"
                     /locus_tag="PL000_09860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNC89248.1"
                     /translation="PSTCNFQPVTKNPVTQMIDNWNMLGHEWAVDMLRQHAARGEARH
                     AYLFCGPPGLGRRTLALHLAQALNCPHPIAPGVPCGTCRDCRQIEAMQHPDMNVVQAD
                     AEGGTLKVDQVREVQRALSLKPYQAKYRVALFLRFQEANDSAANALLKTLEEAPAHAL
                     LLLTADTPEQLLPTIVSRCEILRMRPLPVKAIESYLLDQGVDEERARLLAHISGGRPG
                     YARKLVDDVTLLEKREEYLNDLQTLIPAPRVEKFSYAEKLAKDKDTMRQTFLIWLSYW
                     RDVMLRVAGAETPLINVDRNMEIEFLAGRLDLPTARRVVSDLESALEKMERNVNSRLL
                     AEILLLDWPKA"
     assembly_gap    12165..12264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12336..13244)
                     /gene="rsgA"
                     /locus_tag="PL000_09865"
     CDS             complement(12336..13244)
                     /gene="rsgA"
                     /locus_tag="PL000_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNC89249.1"
                     /translation="MTNEIQKQGLIIKAQSGFFSVDTGEGLVICQLRGKLKQGRAVGD
                     IAALGDHVLITVLADGSGVVEKVEERKQAIVRLDPRPQGEYQQVLLANADQAVFVFAC
                     ANPNPKLRMLDRFLVVAEKQRIPVLIVANKIDLVEDPHETFGLYESIGYRVIYTSTKT
                     GAGLDELKAQIQNKINALAGPSGVGKSSLLNAIQDGLGLAVNEISTAVNKGRHTTVTR
                     QLFALNNGGYVADTPGWKSLALWDTEAEEMDAYFPELRDLVPHCQFSDCTHKHEPGCA
                     VLAALKAGTIHPERYDSYLRLRAGQE"
     gene            complement(13311..15551)
                     /locus_tag="PL000_09870"
     CDS             complement(13311..15551)
                     /locus_tag="PL000_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC89250.1"
                     /translation="MKVFRWINLIVALALLSSCSSGGAEGNPITDFFVTPTPILPTAN
                     PTIIPAPDVQAAVTSFLQALQKDDFETMYAMLAQQSRDALTLEDFSQRWNTALNEMSA
                     ASIEFAINSSVISPYKAEVGYSVTYKTVLAGDIQRNIVMRLANEENAWKVVWDDSLIM
                     PELAGGNVLKMEYSVPARGDIYDRDGLPIVSQADAFAFGIQTDQIDLEMQGALTAELG
                     KLCGYDPEYIKDQIEASGPGNYLPMCEGTREEAQRLLAISPGGLVISDEYNSRYHFGT
                     GIAPQSVGYTQLISPEQLNSYRRLGYNGSESVGQSGIEKWAEDYLAGQHGGILRIVGP
                     NGAIISTLGQTAAEPADSIYLTLDSNMQKYAQRSIEFFRGAVVVMEVDTGRILAIASS
                     PDFDPNYFEGNNPNAGGTYLLPEGALLNRATQGQYPLGSVFKIITMSAALESGLWIKD
                     TPYDCQYDFTRLDQVMHDWTWQHCQDRIARGDFCDSSDSTPSGLLTLQEGLMRSCNPY
                     FWEIGWDLFTNYSRGTDIANMARAFGLGSPTGIAQLEEEGGQITDPQTEVDAVNQAIG
                     QGDMQVTPLQVARFVAAIANGGTLYRPQIVERIQPIDGDPKLVFRPEANGTLPLRSEN
                     LDILREAMLMVTQNPRGTARFNLRGLQFDVAGKTGTAESGNGKSHAWFAGYTLNEANT
                     NLPDIAIAVIVENIGEGSEYAVPIFRAMVETYYYGSPQRIFYDFGPIGEPPYTPTPFG
                     GGNGLP"
     gene            complement(15566..16237)
                     /locus_tag="PL000_09875"
     CDS             complement(15566..16237)
                     /locus_tag="PL000_09875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNC89251.1"
                     /translation="MINVATLVLASNSPRRRQLLALGNWKFDVIVSDVDETQLTGESP
                     KEYVLRLAQAKALAVAGRSNFEAIIIGSDTSVIDGDEILGKPKDETEAERMLKQLRGK
                     THQVYTGVAVHRVSDSKTLTELCITDVPMRNYSDEEIAAYIRTGDPLDKAGAYAIQHP
                     DFQPVESMRGCYASVMGLPMCHVVRMLQQFGISPSAEVPMACQKLLNYACPVSGAILS
                     GVSNG"
     gene            16477..17049
                     /gene="xpt"
                     /locus_tag="PL000_09880"
     CDS             16477..17049
                     /gene="xpt"
                     /locus_tag="PL000_09880"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNC89252.1"
                     /translation="MQLLKDRILRDGKVLGNGILKVDGFVNHQVDPLLMDACGREFAK
                     RFVNVGATKILTAEISGIAPAVTTAVHMGLPVVYARKNKPITMPDQVYLTLAPSHTKG
                     RMVELIVSPEYLENNEKVLIIDDFLASGATILGLVRLAEAAGSQIVGIGALIEKSFEG
                     GRDALASLGVQVESLANIKSLDNNKVEFVD"
     gene            17138..17422
                     /locus_tag="PL000_09885"
     CDS             17138..17422
                     /locus_tag="PL000_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNC89253.1"
                     /translation="MSFLIPVNRLRDTPSLIAFHHPSPGYKFHVLIVPKRQVASLASL
                     DPSDVVFLTDLYSTVQSLVNEFQLKAYRLIVNGGEYQDFPHLHFHLISDT"
     gene            17459..18994
                     /gene="guaA"
                     /locus_tag="PL000_09890"
     CDS             17459..18994
                     /gene="guaA"
                     /locus_tag="PL000_09890"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNC89254.1"
                     /translation="MNSIAILDFGSQYAQIIARRIREVNVYCELFPWDAPQEKILSIN
                     PKGFILSGGPKSVYEENAPYIQKFILESGLPIFGICYGMQALTHALGGEVNPSSEREF
                     GFAEIEPLIPNTMLDVISKVWMSHGDKVTRLPQGFMPLAKSGNSPYAAIGDLQRKYFG
                     VQFHPEVNHTPNGTELLKHFAIEICGAKPEWTPASIIDESVKRIREQVGSQRVLAAVS
                     GGVDSSVAAALVHKAIGDQLVCVFVDTGLLRHNEGEQVASAFRGGLGAELITVEASAD
                     FLGALAGVTDPEQKRKIVGEKFIRIFEQQAKNVGQPKFLVQGTIYPDVVESSAPDRNK
                     ADKIKTHHNVGGLPKDMQFELVEPLRYLFKDEVRLVGEALGLSEALVWRQPFPGPGLT
                     VRCLGECTPERVSRLRAADAILLEELSKAGFLGKGAKTSQAFMVLLPVKSVGVMGDYR
                     TYQEAAAIRAVVTDDFMTADWARLPYDLLAKVSSRVVNEVDGINRVVYDVTSKPPATI
                     EWE"
     gene            19064..19960
                     /locus_tag="PL000_09895"
     CDS             19064..19960
                     /locus_tag="PL000_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89255.1"
                     /translation="MNFDYGDTLKRAAQMTLRYKSFWVLMTLQMLPSMIMFPLFFYAL
                     WVLENSRGEAGGVWMVVLGAALILLFFASFLLMAYSHSAVTLGVIRVDRGGEITDLME
                     LLRDAFPFYGRSLGLILSIQLTIGMIFTVVFMIIGLLMVVTMGIGAICLQPVILLLTP
                     LSMLLMAFMEAAQTALIAEDLGVLDSLKRALQVVRAHVWKYVIISLVVYFGTSIISGI
                     ISIPLTFPAAILAMSTETMSLSDSQMGMAVMASMFCCFVPVIFAISGVTQTFIKTTLG
                     LSYLRLASPAGEQVITLEEQAS"
     gene            20306..21244
                     /locus_tag="PL000_09900"
     CDS             20306..21244
                     /locus_tag="PL000_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89256.1"
                     /translation="MNFNFGEVFTRAWQIIWKHKVLWIFGILASCARGSGGGGGNSGS
                     RYQTSGGDAPFSGGQIEQTFNQIGQYLQDNLWIIVVGIIAILLLGLIFMVLGIVGKVG
                     LIKGTYKADADVERLQFAELWSESLPYFWRIFGLNFLLGLAVFVIIIPFVLLSVFTGG
                     IGALCLLPLICILIPVGFVVSIITEQAQTAMVLEDLGIMDGLKRGWEVVKANIVTMIV
                     MVLILGIGGAIVGIVIALPLIFAALPIVIGMGSLGESLTPLYIALACCVVYLPVLVFF
                     NGVITAYTQSVWTLTFMRLTTPKLKEETPVFVEDNA"
     gene            21237..23153
                     /locus_tag="PL000_09905"
     CDS             21237..23153
                     /locus_tag="PL000_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC89257.1"
                     /translation="MRKFFALLFSLGLLFGAASNVRAQQNLASAELYPADASTFPTVS
                     AFLDVFDTNGIFVSGLKPEAVTVMEDGQQLPVDALTEIAVPLQLTVAVNQGPPLDARD
                     STTNISRYQRISEVLVQWAQSRPADLPDDYSLVSQAGPVINHASAADFVVGLSTFQPD
                     FRAATPNLQSLAIALDTVSAQTTRLGMKRAILFITPHTDDINIASALEPYVQRAIENK
                     IRIFVWFVDLETTFTTTSAAAFNNLAIQTGGSMFKFSGLERFPDPEAYFSPLRRVYAL
                     SYTSRLKSVGEHSLSAKVILPGGQLTSAEQRFVLDIQPPNPFPVTNSLQITRRAPEDD
                     PFNTEVLLPFAQEVEIIVEFPDGHPRALTRTTLYVDGVVMDENTVEPFNLFTWNLAAY
                     TTTGEHQIMVEAVDVLGLSKASMPVPVVVTVVQPPKGISALLAKYRTPITFGAILLAG
                     LVLFAILISGRFRLPSIRAAQEARRAEIDPLTQPIRALGPLPTLAEEKNKKRRSIPKK
                     AATDPRSSAEAMAAFIRVNADGVPAVAAPIQLFEKEITFGTDPVQCTQILDDPSIAPM
                     HARLRQTEDGGFLLLDNGSIAGTWVNYEPIPREGHRLMHGDMVNFGQLSYRFTLRIPP
                     VNSKPKITVHSIEE"
     gene            23150..24019
                     /locus_tag="PL000_09910"
     CDS             23150..24019
                     /locus_tag="PL000_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC89258.1"
                     /translation="MIRSSLPHLHIAALSHAGMSGKNNEDRYAVSSFQLSKDDSRPAL
                     FAVVADGIGGHRAGEVAAELAVNYITAVVAESNAKKPISILENAIHHASQSIAAHSAG
                     KEDEDGMGSTCACVWVIENRLYTAYVGDSRIYLLRGKHIQRLTIDHTWVQEAYEKGII
                     TAEQMHDHPNLHVIRRHLGGIKLPEVDFRLRIDNGETNEESINNQGFHLAPGDTILIC
                     SDGLTDLVWDDEILKTIRSKKDMKASAEALINLANERGGHDNITVVMMTMPYLEETGK
                     KKSGILDWLLGED"
     gene            complement(24162..24761)
                     /locus_tag="PL000_09915"
     CDS             complement(24162..24761)
                     /locus_tag="PL000_09915"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNC89259.1"
                     /translation="MPTFLLPIFSYLCGSLPFSIWVTRFVKKVDVRDAGSGHATTTNT
                     IRQAGFGWGALVFALDVAKGFLPTYLAIQLGASPLILVLTASFAVIGHCWPVFAQFRG
                     GMGLATTGGVLLAINPLAFLVCFGLLLLLVLLIRHSARASVFAGILAAPALWLLNIRD
                     ISMWAALGMGAVIAVRFLIDWNRKYRELWLDRERGIKKP"
     gene            complement(24767..25333)
                     /locus_tag="PL000_09920"
     CDS             complement(24767..25333)
                     /locus_tag="PL000_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89260.1"
                     /translation="MKKIYVLIFAFAFALLISACGSQSVEKSDLSVSCASLQPTENDV
                     KYGLEFGKELFTEVNWQRSYTVKELQSTISWTHRTMSALADVTVYMFCDEVGTDDVTW
                     FYSEDALKIMFEGYDEATLVSSCGKDRLLLYEVDAVEEGQQYKIRLWAEPLNKSRLLS
                     VLLTFPIEESDLLLKYGEEFFPDLPVCP"
     gene            complement(25436..27478)
                     /locus_tag="PL000_09925"
     CDS             complement(25436..27478)
                     /locus_tag="PL000_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC89261.1"
                     /translation="MTSRYDILFQPIQIGPVTAPNRFYQVPHCNGFGWRMPEGMAAMR
                     GMKAEGGWGVVCTEETEIHYTSDLSPFFEGRIWSDDDIPVWQLMTEAVHKHGALAGIE
                     LTYNSHDAANLYSRAAAFGPRSAGITGGSGYEPGQSRAASKEDLKQVRKWHRNAAIRA
                     KKAGFDIVYCYAAHNMTLAYHLLSKDNDRSDEYGGSLENRVRFLRELIEDTKDAVGDT
                     CAVAVRFAVDELLGVDGMQHNGEARDIVGMLAELPDLWDVNISNWANDSVTSRFGKEG
                     HQEKYISFVKKLTTKPVVGVGRYTSPDSMVSAIERGIMDLIGAARPSIADPFLPNKIK
                     ENRHEDIRECIGCNICVTGDTRFVPIRCTQNPTMGEEWRRGWHPEIIAPKKSKDEILI
                     VGAGPAGLEAASALGQRGYHVILTDARREAGGRVLLESALPNLNEWRRVVDYRITKIN
                     KTENIFFYPSSPMTAQDILETGTPHIILATGATWRRDGMGRYLTRPVTGNGKIFTPDD
                     LMNHNLPSGKVIIYDDDHYYMGGVLAELLAQPEAGCEVTLITPAPSISYWTQFTLEQD
                     RILKRMHDLNITLLPNHVIASYDPKAATVTNVITSAETTLPCDAIVMVTDRIPNDTLY
                     HELQPALAEGKIKTLRRIGDAEAPNIIAQAIFSGHLAAREFDETIDPDITPFKVPR"
     gene            complement(27488..28120)
                     /locus_tag="PL000_09930"
     CDS             complement(27488..28120)
                     /locus_tag="PL000_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89262.1"
                     /translation="MKKQIFILIIAAVILSSCGKPPAPPPTSTPLPPPTITSTPRIVA
                     TNTPLPTVTPVCVSPEPTQQDIDRAHSYTGDAFDSSWDQSEAALEGAYSITWQNIVQG
                     AVAYLEARIKPCGYEEPDLNRDFSEATWSAIFANYESYESIAECKLDSGIRLYQFKTY
                     NLGFEYKINYWVESDTDNRIIVTMIVFPAESQPLLDEYSIRLFPNLPNCS"
     assembly_gap    28411..28510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28511..29271)
                     /locus_tag="PL000_09935"
     CDS             complement(<28511..29271)
                     /locus_tag="PL000_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89263.1"
                     /translation="MQTKKLNYDKIASEYNQRYPSTQFWERGQALLELAKRTKSQRIL
                     EAGSGTGFWLNLLHQVTPQLYGMDYSAGMIAQARQQPAPIQLARGTATQLPYQNETFD
                     LVYCVDAIHHFGDHAAFVNEAFRILKPGGTLAVIGHDPHSGDGSWYIYEYFDTVYETD
                     LSRYPSARTIEILMDQAGFRGFQMQNVEHIQNLHVGKAVLNDPFIKHNATSQLALLSE
                     ETYQAGMDKINARLDQAQLENQQLIFKSDFWVRMFT"
     gene            complement(29310..29507)
                     /locus_tag="PL000_09940"
     CDS             complement(29310..29507)
                     /locus_tag="PL000_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HNC89264.1"
                     /translation="MNGQTRANIKPGITVLIVLKQDQRTGKLTKGVVKDILTKSPNHP
                     HGIKVRLMDGQVGRVKEIVPV"
     gene            complement(29504..>29826)
                     /locus_tag="PL000_09945"
     CDS             complement(29504..>29826)
                     /locus_tag="PL000_09945"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC89265.1"
                     /translation="KMTNPPTRPVRYVVIHKPGPKWQYGIDYRQQDGVGEHVEHYLKF
                     HQQGKLQLGGPFLLPDVGGMMVTTKEVSQEEIEAFAAADPAVKSGLLIFEVRPWMTPM
                     EHEQ"
BASE COUNT         6639 a         7278 c         8231 g         7378 t
ORIGIN      
        1 gatgatttcc tcaccaaacc tgttacgctt gccaacctgg ataaattatt taggagatgg
       61 tttacagttg aggcattggg gtaattcttg acgttccccc cttcggcgcg tataatcccg
      121 ctcattcagg agtagtaagc gaaacaagcc tgacagagaa ggatgcatgg gtcaagttct
      181 gaaaggaatc gagaccgaaa tggtggaagc atccacagga actgattgct gaagtcgtct
      241 gatgggtgtg ccgaagaaaa cattgttcaa tgagcagtgg tgagtagaac ggcacgggta
      301 tgcccgttat agcattcaag tgcgtttgtc gaaagataaa cgaattgagg tggtaccgcg
      361 aagcaatact tcgtcctcat gttggatgaa gtatttttat tttaaatgtt ggctgaaaac
      421 gggtagataa gggacaggta tgcaaatttc catgcaggcc tgtttacctc acacaaggag
      481 aaagaatgac cagacaggaa ttgccgaaag cctacgattt caaatccacc gaaccgcgca
      541 tttatgcgat gtgggaaacg ggcggattct tcaagccgca taatgacccc aacaaacctg
      601 gtttcgaccc aagcgtcaaa ccctttgtca tttcgatccc gccgcccaat gtgacgggtg
      661 aattgcatat cggtcatgcc atgttcgtca gcgtggaaga cctgatgatc cgctatcacc
      721 gcatgaaagg gtattccacg ttgtgggtgc caggcaccga ccatgcaggc attgccaccc
      781 agcttatggt ggaacgtgat ctgttgaaaa acgaagaagt gacccgcgaa gaattgggcc
      841 gtgagaaatt cctcgaacgg acctgggagt ggaagaagaa atacggcggc atgatcacaa
      901 accagattcg acgcctgggc gcctcctgcg attgggatcg ggaacgcttc actcttgacg
      961 aaggcctgag tcgggcagtc cgagaggcat ttgtgcgcct gtatgaaaaa ggactgatct
     1021 atcgcggccc gcgcctgatc aactggtcgc cagggttgaa gaccgctgtt tcagacctcg
     1081 aagtggaata caccgaagag aacgccaacc tgtattactt caagtacatg atcgctggct
     1141 ccgatgaatt catccccgtt gccaccattc gtcccgaaac gattcttggg gataccgctg
     1201 tggcagttca ccctgaggat gaccgttaca ggaaattcat cggtaaaaag gccatcgtgc
     1261 cgatcctcgg acgtgaaatc cctgtgatcg ccgatgaata tgtcagcatg gaatttggta
     1321 cgggcgcatt gaagatcacc ccagggcatg acccgaacga ctacgccatc gcacagcggc
     1381 ataatttgcc gatcatttcc atgcttgata aggcggcgaa gatcaatgaa aacggcggcc
     1441 agtataaagg tcaggaccga ttcgaggcgc gcaagaatct gtgggccgat atgaaagccg
     1501 cagacctggt catcaagacc gaaccgtacc gcacgaccat tccacgctca cagcgcggcg
     1561 gtgagatcgt tgagccgatg atctccgaac aatggttcgt gaagatcgaa tcgttggcgc
     1621 aggcagggtt ggacgtggtg cgcgatggac gaatccgaat cgtgcctgaa cgcttcgaaa
     1681 agacctactt caactggctg gaaaacatca aagattggtg tatcagccgc cagttgtggt
     1741 ggggacatcg catccctgtg tggtattgcc ccgaaaacca catgactgta gcacgcgaag
     1801 acccgaccca atgtgccacc tgcggttcaa aggatattcg tcaggatgaa gatgtgttgg
     1861 atacgtggtt ttcatctggc ttgtggcctt tttccacact gggctggccc gaggaaaccc
     1921 ccgacctgaa atatttctat cccacctcct acatggaaac aggatatgac attctgtttt
     1981 tctgggtggc gcgcatgatc atgagtggac ttgaatacac cggacaggcg cccttccaca
     2041 cagtgtacct gcatgggctc gtccgcgatg aacatggaca taaaatgtcg aagaccaaag
     2101 gcaacgtgat cgacccgctc atcatcatgg atgatctcgg taccgatgcg ttacgcttta
     2161 cgttgttggt ggggtccacc cctggaaatg acaccaatgt gggcgtgaag aaagtggaag
     2221 ccaaccgtaa ctttgccaac aagatctgga acgcgggcag gtttgtgatc agcgcgattg
     2281 acgggttgca gcccgggagc gaactgggcg aatataccct ggccgattca tggatctggg
     2341 cccgcctgca gggtctcgtc cgcgatgtgg aacgccagtt ccagaacttc caatatgggc
     2401 aggctggtca gcagatctat gatttcatct ggtctgattt tgcagactgg tatgtggaaa
     2461 ttgccaagca ggaattggcc gaaggcggtg cccgcgcagc cagagccgct gatacattgg
     2521 ctcgtgtctt cgatatggct ttgcgtctgc tgcatccctt tacccccttt gtgaccgaag
     2581 agatctgggg gcacctgcac agcgccctgc gcgaatcgcc gttgaatgag atctgcaaag
     2641 actggcccga tgccctcatc gttgccaaat tccccgagcc gcgtgacccc gaaggctggg
     2701 aagaatccaa gctggctgaa ttcgctttga ttcaagagat tgtccgctcg attcgaaatc
     2761 tgcgtgcgga gaagggggta tctcccagca agaaattcgc cgcgagcatc tcggcaggcg
     2821 caaaagcaga tttgctcaaa gagcaggcac aagtgctcgc atctcttgcg gccttggatt
     2881 ctgcccagct caccatcagc gaagcgttga ctgaaaagcc gaaggatgcc attgcccttg
     2941 tggtgggttc ggtcgagatc tatattccgc ttgcaggcat ggtggatctt gccaacgaaa
     3001 aggcccgcct agaaaaagaa ctcaaggaag ccgaatcgca cattcagcgt ttggagaatc
     3061 tgcttggcgg cgattttgcc aacaaagccc ccgccgcgct ggtggcaaaa gaacgtgaaa
     3121 aattgaatgc ctataaagat acggcagaaa agatcaaggc gcagttgaaa taaaaacagt
     3181 agggacatgg cgccgccatg tccctaccaa tggttgattg cccgatccct cactccaaat
     3241 aaaatatcac ccaattcatt ttattggagg aaccttcatg caaaacccag ttcagtacgc
     3301 tgcctgtccc aagtgtggac aaagcaatgc aaagaaagtc agttacacct tttggggcgg
     3361 cgcactcggc ccatctttga tgacccatgt taagtgccag agctgcggta cagaatacaa
     3421 tggcaaaacc gggcaatcaa accagaagaa cattattatc tacatccttg cgagttttgt
     3481 gatctcattt tgtgtgtgcg gcggtttggc tgttttgaat tttgcattga acagtaacta
     3541 actgacacta ttttagtaag aagctcattg ccgcgaatta caggagtctt tgatatacct
     3601 ttcctgtggt tcgcggcgat gatttttaaa tacgttttgg ataatgttac aaaagcctga
     3661 aaagaaaaga caatagtcaa ttgactggga aaatcatggg gtgtatcttc gtggggcaat
     3721 aaattcaaac ccagaaaagg aatctctgaa ccatgaccat gaaaatccaa gatgactgca
     3781 tcgcctgcgg tgcatgcgta cccgattgcc ccaccgaatc catctcagaa ggcgatggca
     3841 ccctgtatgt gatcgaccag accacctgtg ttgaatgtga aggccatttt gattcaccca
     3901 agtgcgtctc tgtttgcccg gtcgattgca ttgtcaaggc tgagtaacct gcaggtggta
     3961 taatttttac aacaaaataa taaccttcgg ggcagggtga cacttgcacc acaatggcag
     4021 gtgaattccc gatcggtggt aaagcccacg agcctgtatg accgacttaa gtcggtcgta
     4081 cagagcatga cccggtgtaa ttccggggcc gacagtatag tctggataga agaaggtaga
     4141 gcaggcctgt gcaggtactg tgtagattca tgccccgaaa acgaacgttg ttttcgggct
     4201 ttttttctgg atgtgaatcc gcatcacgct tcgcgccgcc ctggcttgct tcacgtgccc
     4261 cgcagttgat tctggcgtgg gcattttttt gttccgctgc cttgtggaaa acctgcaatg
     4321 acaaaactct ttaaccgtat tccctggctt ggattgtttt ccctcgtggc tggcttgctg
     4381 gcatgggatt tgaccgcgcg tctgagcgga ttgcctcgtt ttattttgcc cgcccccgtg
     4441 gatgtgtggc ggagtttcat caaagcgttg accactggaa cgctgttgta tcacacggcg
     4501 gtcacgctga cagagatcgt gcttggcatg ttgacaggcg tgacctttgc cacggtcgtc
     4561 ggttactcgc tggcgaaatc acgtacgctg gagaaggtgt tggccccgta tcttgtggcg
     4621 agccaggcca ttccgatcgt ggcgatcgcg cctttgttgg tgatctggct gggagatgga
     4681 atcctctcga aggtgatcat ctgtgcgttg atcgtgttct tccctgtatt ggtgaatacc
     4741 attgtggggg tgcgggcagt ccctgccgca ctgtatgacc tgatgaattc cgtccgcgcc
     4801 acacgctggc agatcctttt taagttggaa ctgcctgcat ccctgccgat tttgctgggc
     4861 ggattgcgtg tcggcgcgac tttgtctgtg atcggggcca ttgtcggcga gctggtggat
     4921 gcacaggaag ggctgggata cctgttgcag ctgggcgatt ttcaatatga tacgtccatg
     4981 gtgtatgtgg cggtgtttgt gctgatcttc ctcgcacttg ggttgtatgg aatcgtaacc
     5041 ctgttggaaa aaagattctt gaaatggcaa agcacttcgt cctcttgatg aggcatcgca
     5101 gttgaagaat aaaaaatgaa ccgcaaagct cgcaaagaag agcaagaaaa cctggcgatc
     5161 tgcgcggtaa gaaaaaaata taggagacgt aatatgttga agaaattagt ttatctcatg
     5221 cttgggctgg cgatcggcct ttcggcctgc accagttcac agggcggaag tgaagcgggc
     5281 acgcttcgca aaatcaaact gccgatgggg tacattccca atattcagta tgcgccgttt
     5341 tatgtcgcag tcgaaaaggg atattttgcg gaagaaggca ttgagatcga attcgattat
     5401 tcttttgaga ccgatggcgt ggcattggtg ggtgcggggg aactgccgtt tgctgtggct
     5461 tcgggtgaac aggtgctgct ggcccgttcg caggaattgc ctgtagtcta tgcgtttgcg
     5521 tggtatcagc aatttcccat ttcgatcgta tccacccctg aattgaacat cacgaccccc
     5581 gctgatttga aaggacagac cattggcctg cctggtttgt ttggcgcgaa ttacattggt
     5641 ttgcaggccg tgttgtttga agcaggcctc acacccgatg acgtaaagat ggatgcgatc
     5701 gggtttaatc aagtggaggc atttgccacg aaacagaaga atgtagtggt ggggtatgcc
     5761 gcaaatgagc cgatcgtcct gcgttcgcaa ggcatggagg ttgttgagtt gcgggtttca
     5821 gattatgtgc agcttgccgc gaacggaatt atcaccagcg agatggcgat caaggatgaa
     5881 cccgatcttg tgcggggcat ggcgcgggca ctggcacacg gcattgccga taccattgcc
     5941 aaccctgaag aggcatacaa gaccagcgaa aaatttgttg aaaaccttgc cgaacaggat
     6001 aaggatttgc agatgcagat cctgacgaca tccattgagt tttggaaggc cgagaagatc
     6061 ggttattcag acccgagtgc ctgggaaaat atgaacgacc ttttggtgaa aatgggcttg
     6121 atccccgaac tggtggacct gaccaaagcc ttcaccaatg agttcgtgcc gtaatgtaga
     6181 ggccgattct cgatccatcg tgatcactca ccttgcgaag ttgtaaccgc ggcctgccgc
     6241 ctgcggtcag gtatttttca aagcttgttt ttcgctgcgt aaggtgagta acccatcctt
     6301 tcaggaattg gcatcggaag ttggaaacca tgaccattaa gcccattctt actgttaaaa
     6361 atatttcggt tgtctttcca gatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnncatgacc attaagccca ttcttactgt taaaaatatt tcggttgtct ttccagatga
     6541 taacggcggt ttgcgcgcgt tggatgatat ttcattcgag gtgcgcccgc aggaattcat
     6601 ttgttttctt gggccgtcgg ggtctggtaa gaccacactg ctgcgcatcc tggcggggct
     6661 tttgccgccc acagcggggc gggtaaattt cattcgcaac caacaccctg agatcggcat
     6721 ggtctttcag caatcgaatc tcatgccgtg gcgaacggtg atggacaata ttaaattacc
     6781 gcttgagttg gaagggatgg gggagacccg ggcacgcatc aaggcgcgtg aaatgatcga
     6841 tctggtcggg ctgaatggat ttgaaaactc ctggccgagg gaattatctg gcggaatggc
     6901 acagcgggtg gcgatcgctc gcgcgctcat ccatgaccca gacctgctgc tgctcgatga
     6961 gccttttgct tcgctcgatg ccctcacccg cgaacgcatg tggatggaac tttcgcgcat
     7021 ctggcaggcg cgccagaaaa ccgtcatcat ggtgacccat tccatcaacg agtcgctctt
     7081 ccttgcagac cgcgttctgg tgctgacgca gcgccccggt aaaataaaac tggacgtgga
     7141 ggttgacctg ccacgtccac gcaaggacga tatccgttac acgccgcatt ttggaaaact
     7201 ggcgcggaaa ttacgcgaga ccatcgagtg atcggttgat cttcatttca ttttgaggca
     7261 ttaaacttcc caagccagcc gccgaaagaa tgacaactgc gggcatgaga tcgaccagat
     7321 agcgcatctg ggcgtcaatg gccatgtatg ctccgcatcc caaagcaagc gcaaggataa
     7381 acggccacat gctgcgccag tgtttcgaaa ttccaaacag tacggccagg agaaacaaga
     7441 actgctgaac gtacgagaga tagtctgcca tgtagtaacc cgcgccatat gcttcgttga
     7501 cgctgacgtt gaaccataaa ggcaggaaac ggtacagcac caattgtaaa tactcaatgg
     7561 ggtgctgggt aataagctgc agaccagcct tcatgtaaat gctttgcagt tgagcttcgt
     7621 tttcaagacc cgtcaacgta ctgtggctca acaggtctct gaccgctgca tacccttctt
     7681 tagcgccgac ataatgtggt tggaaatcgg cattggcgac gatgaagttc atgcggtaaa
     7741 tattgtagcc agtcaacgag ctgccgatga cgggctgccc aaaaacgagt gtgttccgaa
     7801 tgacccacgg ggaaaagaca agaaaaaggg tcgccagaaa caacaggatt ccaccaatct
     7861 gttttttgga aaacggtctg gtttcgaacc atttttttgc cagcataacc gcccctaaac
     7921 cgattccaac ccccagcaaa acggtccgcg agagggcggc cagcccaaag aacagacctg
     7981 agatcaccca atctttggct ttcgatgcat gatatcccct taagaagaaa tacattccga
     8041 aggaaaaaaa gaacgcggcg gcaagatcac ctgagccatt atccacctga ttaatgacgg
     8101 ggacagagaa ggtccacatg agggtgccga gcagggcggt tttcacattg cggctggatt
     8161 ctttcagggt taagtagatc gcaggaatgc ttcccagata gtacagactt tgcatgatca
     8221 gtgctgaaag tttggtggga tggaaaagcc tggccaatgc gatcaacagc acgggaatgg
     8281 gctcacgcat ggccgtggcc tggctggtgg attcgcaatt gggaaagtaa tcggtggcgc
     8341 atccgctgta gccttttccg ccgaccagat tcgatgcgat ggcccaggca ccgccgtcac
     8401 ttttgaagat caagttgccg ttaaagtatg catgaaagat tggagacagg cacagcatgg
     8461 caatgagaat gactcctgcc agccaatact tttttgtgta atcccatgaa aagatgggtc
     8521 tgaagacatc aagcatttga aatactcccc tgtgcggttt gatatcttcg atttgatgcg
     8581 tatgcgattt catgcctgaa ggatacccgt tggagatgac agtaaaatga cgaataattc
     8641 cgtcccgtcg tctctttatg ttttcaggtc gtggagattg cattgagggg cctttacctg
     8701 aagaaatgcc ccggggagca agcgggaaaa tcagatcggt atcccgtcta cgatctcctg
     8761 gcggtgtttg cggtagtcgc ggatgagcga gtcgacacag cgtgccactg tggcagggcc
     8821 gccgcgataa tggatgatgc ctgtatccgc ttcccattca ctttcaggga tggtcttcaa
     8881 ataggcggtc agtatttcct ttgtggcggc gatatctctt aacagtgtat cacggtcggt
     8941 ggctgaaaac tggctaagaa atttcgcgtt gaccttgcgg tagtcgttcg tgccatcgta
     9001 aaaataaaat ggttcggcac cttcacgcag ggcgctgcac cccgccagtg tgatgcggtt
     9061 ccagccaatg aaatgcgcgg cgatgtcgcg cggggtccaa tcgccgaggg tggtgaaaaa
     9121 tgcttcggat gccagaccag ttaccgtgtc gatgaaagag gcgtaggcgg tttcaaggtc
     9181 cgcgagttgt ttttccttgt gatcgaacat ggcggctcct gtttatttca aacttttata
     9241 tccagtaaaa cccatgccaa gcagggcaat tgcgctggcg aaaataaaaa acggcacaag
     9301 caaaaatcct gatacttctt tgaggctggt gccgttgagc gcccaggatg cgacgaagca
     9361 gcagagacca atatgtagga ctcccgccaa aatggagagg accgacccga tcaagcggat
     9421 cattcgtacg ggcaggctgg tcagccctgc gaggagcaga atcagcgccc catacggaat
     9481 gaacaccgcg ccaaagaccc cgcgaaaggc acagccaagg tcggcataac ttttacagtc
     9541 ctgcccatcg gcgaccagtt gaattccaaa accgatgacc gcgattccgc acagtgctgt
     9601 gatcgcggca gagacccaat agacggggtg cggtttgttc atctggcctt gagtgcggca
     9661 tccacttcgt tgagatgttc gttgcgatgc aaagcacgga caacccagcg tttgttgtag
     9721 ttgtagattt cttcaagcaa atcctgcgga aaggcttcta gctgagcatc gagatcggcg
     9781 gaggattcaa tggcgatccg tgcggcttca cgcgggggaa tggccgccca caacggcagg
     9841 gacaggtcat tgacgatgat gtcaatctca gggacgaata atttgccgtt cttcacggtc
     9901 atgtccagca catacattac acggcgatcc caccaggcga ggtgggcgag ggcaatggcc
     9961 actgtccaat gctcgccgac gcgcgtttgc atttcttcat cgctaaggct ggccgccagt
    10021 tgtttgatgc gttccgtgga ggcacggtta agttgtgtgt aattggggtc aagtgacatg
    10081 ggaaactcct attcttgaaa ttgttttacc tgtaatgaac catttttcaa caattccaac
    10141 atcctgcgcg tggactcggc tacttcgtcc acggcttttt gaaatgccgc ttcgtttgcc
    10201 ttggatggtt tgcgatatcc gctgaccttt cgcacgtatt gcagtgcggc ttcctgaatt
    10261 tcctgttcgg taacagggtg gtccatattt cgcaggggtt taatgcttcg gcacatgata
    10321 agtctcctgt tgataaagtg ttactgtact atccatttgt ttaataaagt tctcagcttc
    10381 aacgattttt gtataacttc ttcgctttca atatttccaa cagaaatgat ggatggaagt
    10441 tccctgtata gttcggggag gcccagtttc tgttccactt gttcgcggat ccacttgttg
    10501 taggcgattg ggtaggtctt tctgctaata aaaagtgcct gtaaaaattc ttgaaaggct
    10561 ttgtataaac ggtcaaatgc ttgaaagtat aatccgcggt gaaaaaagaa agggacatga
    10621 tccagatcat acgtacaagc gtgacaggtc attgccagtc gttgggcttg caatggaggg
    10681 tcataatatg gaagccaggc ggattgaagt tgactgtaat aagagccgat ctcgtgcatg
    10741 ggagccccat aagccagctg atttccaact tccaattcga agccgtccgg gccgccgcca
    10801 tcatcccaaa tggatggagt gtactggcct gtaatcagat caaggtgcaa ctgcatgaag
    10861 ggatgtgaga ctttgaattg ctgaaacaca gggttggatt gcaaggcagc ctgccacttt
    10921 gtttccaatt cttgcatatc tgagtgggcg gtttcaggtt tcacaaggac cgccatgtca
    10981 aggtcgcttt caggtgtggc ctgtccacgg gcgcaggaat tgaccaccaa aaccgtatcc
    11041 accgttgatt ggttgagaaa gaaatcgcgc gcgaactccg ccgttttttg atgcagcagg
    11101 gttgggaatt tcggctgtgg attcatttat gcctttggcc aatccaacaa caggatttct
    11161 gccagtaaac gtgaattgac gttgcgttcc attttctcca gtgcagattc caggtcactg
    11221 accacgcggc gggcggtggg gaggtcgagg cgtcccgcga ggaattcgat ctccatattg
    11281 cggtccacgt tgatgagcgg ggtttccgca ccagccacac gcagcatcac atcacgccag
    11341 tatgaaagcc agatcaaaaa ggtctggcgc atggtgtctt tatctttggc gagtttttcg
    11401 gcgtaggaga atttttccac acgcggggcg gggatgaggg tttgcaggtc gttcaggtat
    11461 tcttcgcgtt tttcgagcag ggtcacatcg tctacgagtt tgcgggcata gccggggcgg
    11521 cctccgctga tgtgggcaag caggcgtgcg cgttcctcat ccacaccttg atcgagcaga
    11581 taggattcga tggctttgac gggcagcggc cgcattctca aaatctcaca gcgcgaaaca
    11641 atggtgggca ataattgttc gggcgtgtcg gcggtcaaca gcaaaagggc gtgggcgggg
    11701 gcttcttcca gcgtttttaa taacgcgttg gcggcactgt cattggcttc ctgaaaccgc
    11761 aggaacagag ccacacgata ttttgcctga tacggtttaa gcgaaagtgc gcgctgcact
    11821 tcacgcacct gatccacttt gagcgtaccg ccttcggcat cggcctgcac cacattcata
    11881 tcgggatgtt gcatggcttc gatctgcctg caatcacggc acgttccaca gggcacaccg
    11941 ggggcaatcg ggtgcggaca gttcaatgcc tgggcgaggt gcaaagccaa tgtgcggcgc
    12001 cccagtccgg gaggtccgca gaaaagatac gcatggcgtg cttcgccgcg tgccgcgtgc
    12061 tggcggagca tatccaccgc ccattcatgt ccgagcatgt tccagttatc gatcatttgg
    12121 gttacagggt ttttggttac aggttgaaag ttgcaggttg aaggnnnnnn nnnnnnnnnn
    12181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnncgatca tttgggttac agggtttttg gttacaggtt
    12301 gaaagttgca ggttgaaggt tgcaggttgg agggattatt cctgcccggc gcgcaggcgc
    12361 aagtacgaat cgtatcgttc gggatgaatt gtacctgctt tcaatgcggc gaggacggca
    12421 cagcccggtt catgtttgtg cgtgcaatcg ctgaattgac aatgcggcac aaggtcgcgc
    12481 aattcgggaa aatacgcatc catttcttcg gcttcggtat cccacaatgc caggcttttc
    12541 cagcctggtg tatcggctac gtatccgccg ttgttgagtg cgaacaattg acgtgtgacg
    12601 gtggtatggc ggcctttgtt tacggctgtg ctgatctcgt tgacggccaa tcccagccca
    12661 tcttgaattg cattcagcag gctggatttc cccacgccgc tcggccccgc cagcgcattg
    12721 atcttgtttt gtatttgcgc cttcaactca tcgagccctg cacctgtttt ggtggaggtg
    12781 tagatcacgc gatagccgat cgattcatac aagccgaagg tctcatgcgg gtcttccaca
    12841 aggtcaatct tatttgccac gatcagcaca gggattcttt gcttttcagc cacgaccaga
    12901 aaacgatcga gcattcttaa ttttggattt ggattcgcac acgcgaaaac gaaaacggcc
    12961 tgatcggcat ttgccagcaa aacctgctga tactcaccct gaggccgcgg atccaacctg
    13021 acgatggcct gcttgcgctc ctcgaccttt tccaccactc cggaaccatc tgccaaaacc
    13081 gtgatgagca catgatcgcc cagcgccgca atatcgccaa cggcacgccc ctgtttgagt
    13141 tttccgcgca gctgacaaat gacaagaccc tccccggtat ccacgctgaa gaaaccagat
    13201 tgggccttaa tgatcaaacc ttgtttttga atttcgttgg tcaaatgttt ttccttgggt
    13261 aagtaggcct gacagatctt cgaagacctg tcaggccttt gcttgtaagt ctatggtaac
    13321 ccgttgccgc cgccgaatgg ggtgggggta tagggtggtt caccgatggg gccgaaatcg
    13381 tagaagatgc gctgtggaga gccgtagtaa tacgtctcaa ccatggcgcg gaagatgggc
    13441 actgcatact cggacccttc gccgatgttt tccacgatca ctgcaatggc aatatcgggg
    13501 aggttggtat tggcttcgtt gagcgtgtag ccagcaaacc atgcatgaga tttcccattg
    13561 cctgattcgg cggtgccggt cttgcctgcc acgtcaaatt gcaggccacg cagattgaag
    13621 cgggccgtgc cgcgcggatt ttgggtcacc atcaacatgg cctcgcgcag aatgtcgagg
    13681 ttttccgagc gcagagggag cgtgccattg gcttcagggc ggaaaacgag tttggggtcg
    13741 ccgtctatgg gctgaatcct ctcaacgatc tggggcctgt acaaggtccc gccgtttgcg
    13801 atggcagcga caaatctggc gacttgcaag ggtgtcactt gcatatcgcc ctgcccgatg
    13861 gcttggttca cagcatccac ttctgtttgc gggtcagtga tttggccgcc ttcttcttcc
    13921 agctgtgcaa tgccagtggg agagccaagc ccgaaggcac gtgccatgtt ggcaatatcg
    13981 gtgccacggc tatagtttgt gaataggtcc cagccgatct cccagaagta gggattacag
    14041 gagcgcatca gtccttcttg cagggtgagc agacccgagg gggtgctgtc tgaagaatcg
    14101 cagaagtcac ctctggcaat gcgatcctgg caatgttgcc aggtccagtc gtgcatgacc
    14161 tgatcgaggc gggtgaaatc gtattggcaa tcgtaaggcg tgtctttgat ccaaagtccg
    14221 ctttcgagcg cggcagacat ggtgatgatc ttgaagaccg agccaagcgg gtactgtccc
    14281 tgtgtggcgc ggttgagcaa tgcaccttcg ggcagaaggt aggtgccgcc cgcattcggg
    14341 ttgttgcctt caaaatagtt gggatcaaaa tcaggcgagg aggcgatcgc aagaatccgc
    14401 ccggtgtcca cttccatcac caccaccgca ccgcggaaga attcgatgga gcgctgcgcg
    14461 tacttttgca tgttgctatc gagggtcagg tagatggaat cggcgggttc ggcagcggtt
    14521 tggccaaggg tggagatgat ggctccgttg gggccgacaa ttcgcaaaat gccgccgtgt
    14581 tgccctgcca aatagtcctc ggcccatttt tcaatgccag actggccaac gctttcactg
    14641 ccgttgtaac cgaggcggcg ataggaattc aattgctcag gggaaatcag ttgggtgtac
    14701 ccaaccgact ggggcgcaat acctgtgccg aagtgatacc gcgagttgta ttcatcagag
    14761 atgaccaatc cgcccgggct gatggcaagc aggcgttggg cttcttcgcg ggtgccttcg
    14821 cacatgggca ggtaattgcc gggaccgctg gcttcgatct ggtctttgat gtattcaggg
    14881 tcgtagccgc ataacttgcc aagctcggcg gtcagggctc cctgcatttc gaggtcgatc
    14941 tgatcggttt ggatgccgaa tgcgaaggca tcggcttggg aaacgatggg caggccatcg
    15001 cggtcataga tatcgccgcg tgcagggacg ctgtattcca ttttgagtac attgcctccc
    15061 gccaattcag gcatgatgag gctgtcgtcc caaacgacct tccaggcatt ttcttcattg
    15121 gcaagacgca tgacgatatt ccgttggata tcgcctgcca gcacggtttt gtaggtgacg
    15181 ctgtatccca cttcggcctt gtatgggctg atgaccgacg agttgatcgc aaattcgatg
    15241 ctggccgcgc tcatttcatt caaagcggtg ttccaacgct gtgaaaaatc ttccagggtc
    15301 agtgcgtcgc ggctttgctg ggcgagcatg gcatacatgg tttcaaaatc atctttttgc
    15361 aaagcctgca aaaatgacgt gactgccgcc tgcacatccg gtgcgggaat aatggtgggg
    15421 tttgcggttg gcaggatggg agtgggggtg acaaaaaaat cggtgatcgg gttgccttct
    15481 gctccacctg aactgcaaga cgaaagcagg gccagtgcca cgatcaaatt tatccatcga
    15541 aaaactttca ttcgtttcct ctcaatcaac cattcgatac gccgctcagg atcgcgcctg
    15601 aaacaggaca tgcatagttc aataattttt gacaggccat cggtacttct gccgagggag
    15661 atatgccgaa ctgttgcagc atccgcacga catggcacat gggcagcccc atgacactgg
    15721 cataacatcc gcgcatggat tcgacaggct ggaaatcggg gtgctgaatg gcatacgcgc
    15781 cggccttgtc gagcgggtcg cctgtgcgaa tgtaggcggc gatttcctcg tctgaataat
    15841 tacgcatcgg cacatctgtg atgcacaatt ccgtcagtgt tttgctgtca ctgacgcggt
    15901 ggaccgcgac gcctgtataa acctgatggg ttttgccgcg caattgtttg agcatgcgtt
    15961 cggcttcggt ttcgtctttt ggcttgccaa gaatctcgtc gccgtcgatc acggatgtat
    16021 ctgacccgat gatgatggct tcaaagttcg acctgcctgc aacagccaat gctttggctt
    16081 gagcgaggcg caatacatat tcttttggtg attcgcctgt cagttgggtc tcatccacgt
    16141 ctgagacgat gacatcaaac ttccagttcc caagtgcaag cagttgtctg cggcgcggag
    16201 agttggatgc cagcacaagt gtggctacat ttatcatgcg caagattcta acacagactt
    16261 ttttacgact ttatggaggc cagtcccatg gattcaccgg cgaaaaacga ccgcggccag
    16321 tgttttacgt ctgattcttt gcagtagatg taaaaaaata ggcatggtct tttctgcttt
    16381 catcgcgtgt ctgccgaatt cgctgttacc cactggcagg ttattccttc acgttgtaca
    16441 atgtgagaaa atctgtctag tcaatgtgga ggcaacatgc agttactgaa agatcgaatc
    16501 ttgcgggatg ggaaagtatt ggggaacgga attttgaaag tggatggttt tgtcaatcat
    16561 caggtggatc cgcttttgat ggatgcctgc gggcgggaat ttgcaaaacg atttgtaaat
    16621 gttggtgcaa ccaagatcct cacggctgaa atttcaggta ttgcgcctgc ggtcaccact
    16681 gctgttcata tggggctgcc cgtggtgtac gcacgcaaga acaaacccat taccatgcca
    16741 gaccaggtat atctcaccct cgcgccttcg cacacgaagg gacgcatggt cgagttgatc
    16801 gtgtctcctg agtatctcga aaataacgaa aaggtcttga tcattgatga ctttctcgct
    16861 tcgggtgcaa ccatccttgg gttggtgcgg ttggctgaag ccgctggctc acagatcgtg
    16921 ggtatcggcg cgctgatcga gaaatccttc gaaggcggcc gcgatgcgct cgcgtcactg
    16981 ggtgtgcagg ttgaatcgct ggccaatatc aaatcactcg ataacaacaa agtcgagttt
    17041 gtggattaat cacaaattac atttcgcgcg tgacccattg cggaaacttc ttcggcttcg
    17101 gcttgtttcc cgttttatcg gctggatttt cacctacatg agttttctca tcccagtcaa
    17161 ccgcctccgt gataccccaa gcctgattgc gtttcatcat ccttctcctg gctataagtt
    17221 tcacgtgctc attgtcccga agcggcaggt ggcctctctt gccagtcttg atccatcgga
    17281 tgtcgttttt ctaaccgacc tgtattccac ggttcaaagt ttggtgaacg agtttcagct
    17341 caaagcctac cgtctcattg tgaacggtgg ggaatatcag gattttccac atttacattt
    17401 tcatcttatt tcagatacgt agggacacgg caccgctgtg tctttacagg gattaattat
    17461 gaactctatt gccattcttg attttggttc tcaatatgca cagatcatcg cacgccgcat
    17521 ccgcgaagtg aatgtgtatt gcgagttgtt tccgtgggat gcaccccagg aaaaaatact
    17581 ctccatcaat cccaagggat tcatcctctc aggcggaccg aaatctgttt atgaagagaa
    17641 tgcaccttac atccagaaat tcattcttga atcaggcctg ccgatctttg gtatttgtta
    17701 cggcatgcaa gccctgaccc atgcactggg cggggaggtc aacccttcaa gcgagcgcga
    17761 atttgggttt gccgagatcg agcctttgat ccccaatacg atgcttgatg tgatctccaa
    17821 agtgtggatg tcacacggcg ataaggtgac acgcctgccg caggggttca tgccgctcgc
    17881 caaaagcgga aacagtccat atgcggccat cggtgatttg caaagaaaat atttcggggt
    17941 gcaatttcac cccgaagtga atcacacccc gaacggtacc gaattgctca aacactttgc
    18001 cattgagatc tgcggcgcaa aacccgaatg gacgcccgct tccatcattg acgaaagcgt
    18061 gaagcggatc cgtgaacagg tggggagtca gcgtgttctg gcggcggttt cgggcggcgt
    18121 ggattcgtcg gtggcggcgg cattggttca taaagccatt ggcgaccagt tggtgtgtgt
    18181 gttcgtggat acgggcctgc tcaggcacaa tgaaggtgaa caggtcgcct ctgcgtttcg
    18241 gggcgggttg ggcgcggaat taatcactgt ggaagccagc gcagattttc ttggtgccct
    18301 tgccggggta actgaccccg aacaaaagag gaagatcgtg ggggagaaat tcatccgcat
    18361 ttttgaacag caggcgaaaa acgtcggcca gccgaaattt ttggtgcagg ggacgatcta
    18421 tcctgatgtg gtggagtcgt ctgcgcctga ccgcaacaaa gccgataaga tcaagaccca
    18481 tcacaatgtc ggcgggctgc cgaaggatat gcaatttgaa cttgttgagc cgttgcgcta
    18541 tttattcaag gacgaggtcc gtttggtggg ggaggcgctc gggctgagtg aagctctcgt
    18601 ttggcggcaa ccgtttccag gtccaggctt gaccgtgcgc tgcctcgggg agtgtacgcc
    18661 ggagcgtgta tcccgtttgc gggcggcgga tgcgatcctt ttggaggaat tatccaaggc
    18721 aggcttttta ggaaaaggcg caaagacctc acaggctttt atggtgttac tgcccgtgaa
    18781 gtcggtgggg gtgatgggcg attaccgcac ctatcaggag gcggctgcca tccgtgcggt
    18841 ggtgaccgat gactttatga ccgccgattg ggcgcgcctg ccgtacgatt tgctggcgaa
    18901 ggtttcaagc cgtgttgtca atgaagtgga tggaatcaat cgtgtggtgt atgatgtgac
    18961 cagcaaaccg cctgcgacga ttgaatggga atagcatttg taacgcggac tttagtccgc
    19021 accggctttg agaaaatgat tctacgattg atgagggact gccatgaact ttgattacgg
    19081 tgatactctg aaacgagccg cacagatgac cttgcggtat aaatcctttt gggtgctgat
    19141 gacattacaa atgctgccat ccatgattat gtttcctctt ttcttttacg cgctttgggt
    19201 tttggagaac agccgcggcg aagctggggg tgtctggatg gtcgttttgg gggctgcttt
    19261 gatcctgctt ttttttgcca gttttcttct catggcatat tcccattcgg cggtgacttt
    19321 aggagtgatc cgtgtggatc gcggcggcga gattaccgat ctgatggaat tgctgcgtga
    19381 tgcgttccca ttttatgggc gcagtttggg tttgatcctg tccattcagt taacgattgg
    19441 gatgatcttc acggtcgttt ttatgatcat cgggttgctg atggtcgtga ccatgggcat
    19501 tggtgccatc tgtttgcagc ccgtcatcct gctgttgact cccttgtcca tgctgttgat
    19561 ggctttcatg gaagccgcgc agacagcctt gatcgcggaa gacctcggcg tgctggattc
    19621 tttgaaacgc gccctgcagg tggtccgcgc ccatgtgtgg aagtatgtca ttatttcgct
    19681 ggtcgtgtat tttggcacat ccattatttc cggtatcatc tccatcccgt tgacctttcc
    19741 tgccgccatc ctcgccatgt ccactgaaac gatgtccctg tctgattctc aaatggggat
    19801 ggctgtcatg gcttccatgt tttgttgttt tgtccccgtt atctttgcca tttccggtgt
    19861 gacccaaacc tttatcaaga cgaccctcgg tctttcctac ctgcggctgg ccagcccggc
    19921 gggggagcag gtcatcaccc ttgaagaaca agcctcctaa aactgcgata cccaaccaca
    19981 gaacgcacag agatcacagg taaccctgtg attctgtgct ttttgttata agatgttttt
    20041 ctacctgact cgtattcccc cgtttgggct acttcgtaaa ctgcaacctc aaattgtttt
    20101 ggacgtaaga atccatgttt gatgttgaaa aatcggtcga tttggcccta aagttacaaa
    20161 tccaaaaatc cctgcgtgga cggatttaat cgggtggtgt atgatactgg tcagttcctg
    20221 acctttgtaa ttttgggcac gaatttagag ttgattttca gcacactttt gcgaaaaatc
    20281 tgaaaaaatg agatgaggag tccctatgaa tttcaacttt ggagaagttt tcacccgtgc
    20341 ctggcagatc atttggaagc acaaggtatt gtggatcttt ggtattctgg caagctgtgc
    20401 ccgaggaagt ggcggcggtg gagggaactc cggttcgcgg taccaaactt ccggaggcga
    20461 tgctcccttt tcaggcggac aaattgagca gacctttaat caaatcgggc aataccttca
    20521 ggataatctc tggatcatcg ttgttggtat cattgccatc cttttgcttg gactcatttt
    20581 tatggtcctt ggcattgttg gcaaagtcgg cctgatcaaa ggcacttata aagccgatgc
    20641 ggatgttgaa cgcctgcaat ttgcagaact gtggtctgaa agcctgcctt acttctggcg
    20701 catctttggc ctgaacttcc ttttggggct ggcggttttt gtgatcatta ttccctttgt
    20761 tttgctgagt gtgttcacag gtgggattgg cgcgttatgt cttttgccgc tcatctgtat
    20821 tttgatcccg gtggggtttg tagtctcgat catcacggag caggcccaaa ctgccatggt
    20881 gcttgaagac ctcggtatca tggacggtct caaacgtgga tgggaagttg taaaagccaa
    20941 tattgtcaca atgatcgtta tggttttgat ccttggcatt ggtggtgcaa ttgtcggcat
    21001 cgtcatcgct ttgccgctga tttttgccgc gcttccgatt gtgattggaa tgggttctct
    21061 gggtgagtcg ttgacacctt tgtacattgc actggcttgc tgcgttgtct atttaccggt
    21121 gctggtattt ttcaacggcg tcatcaccgc ctacacccaa tctgtgtgga ccttgacctt
    21181 tatgcgtctg accaccccca aactcaaaga ggaaacccct gttttcgttg aggataatgc
    21241 gtaagttctt tgctttactg ttcagtttgg ggcttttgtt tggggccgct tcgaatgtgc
    21301 gtgcccagca aaaccttgca tccgcagagc tttaccctgc ggatgcttcc actttcccga
    21361 ctgtttccgc ctttttagat gttttcgaca cgaacggcat tttcgtatcc ggactcaagc
    21421 ccgaagctgt taccgtcatg gaagatggac agcagcttcc agtggatgcc ctgacggaga
    21481 tcgccgttcc tctgcaattg accgttgctg ttaatcaagg tccgcctctc gatgctcgtg
    21541 actcgaccac gaatatttcc cgctatcagc gcatttcaga ggtgctggtt caatgggccc
    21601 agagccgccc cgccgacctg cccgatgatt acagccttgt cagtcaggcg gggccagtca
    21661 ttaaccatgc cagtgccgcc gattttgtcg tggggctgag cacgttccag cctgatttcc
    21721 gagccgctac tcccaacctg caatcgcttg ccatcgcgtt ggacacggtc agcgcacaga
    21781 ccacccgttt gggtatgaaa cgcgcgatcc tgttcatcac tccgcacacc gacgacatca
    21841 atatcgcctc ggcgcttgag ccatatgtgc agcgagccat cgaaaacaag attcgaatct
    21901 tcgtctggtt cgtggacctg gaaacgacat tcaccaccac cagtgcggcg gcattcaaca
    21961 accttgccat ccagacaggc gggagcatgt tcaaattctc cgggcttgaa agattcccag
    22021 acccggaagc gtatttctcg cctttgcgcc gtgtgtacgc actttcatac acctcacgtt
    22081 tgaaatcggt gggggagcac tcgttgagtg ccaaagtgat tttgcccggc ggacagttga
    22141 cctctgccga gcagagattt gtcctcgata ttcagccgcc gaatccattt cctgttacga
    22201 actcgctgca gatcacaagg cgcgcccctg aagatgaccc attcaatacg gaagtgcttt
    22261 tgccatttgc gcaggaagtg gagatcattg tcgaattccc cgacgggcat ccccgcgctt
    22321 tgacccgcac caccctgtac gtggatggcg tggtcatgga tgaaaatacc gtcgagccgt
    22381 tcaatctatt cacgtggaat ctggcggctt acaccaccac cggtgaacat cagatcatgg
    22441 ttgaggcggt ggatgtgttg ggattgagca aggccagcat gcccgtccca gtggtggtga
    22501 cggtggtaca gcccccgaag ggaatttctg ccttgctggc gaagtatcgc acccccatca
    22561 cctttggcgc gatcctgttg gcggggcttg tgctatttgc catcctcatc agcggaagat
    22621 tccgcctgcc atccatccgc gctgcacagg aagcacgccg cgctgaaatt gacccgctca
    22681 cacagccgat tcgcgccctg ggtccgctgc ccacgctggc agaagagaaa aacaaaaagc
    22741 gccgaagcat tccaaagaaa gccgcaacag atcccaggtc aagtgcagag gcgatggctg
    22801 ccttcatccg agtcaatgcc gatggtgtgc ctgctgtggc ggcgccgatc caactctttg
    22861 aaaaggaaat cacctttggt accgatcctg tgcaatgcac tcagatttta gatgacccct
    22921 ccattgcgcc catgcatgcc cgcctcagac agaccgaaga cggaggattc ctgcttttgg
    22981 ataacggctc gatcgccggt acctgggtga attatgaacc catcccccgc gagggccatc
    23041 ggctgatgca cggggatatg gtaaactttg gacaattaag ttatcggttc acgcttcgca
    23101 taccaccggt aaactccaaa cccaaaatta cggtccacag catcgaagaa tgattcgttc
    23161 ttcccttcct cacctacaca ttgcagcact tagtcatgcc ggtatgtctg gcaagaataa
    23221 tgaagaccgt tatgcggttt catcattcca gctaagcaag gatgattcgc gcccggcgct
    23281 ctttgccgtg gtggcagatg gcattggcgg gcatcgcgcc ggggaagtag ccgccgaact
    23341 ggcggttaac tacatcaccg cggtcgttgc cgaaagcaat gccaaaaaac ccatcagcat
    23401 tctcgaaaat gccattcacc acgccagcca atccattgcc gcgcactcag caggcaaaga
    23461 ggatgaagac ggcatgggct ccacctgtgc ctgtgtctgg gtgatcgaga atcgcctgta
    23521 cactgcctat gtcggtgatt cgcgtattta tctgttgcgc ggcaagcaca ttcagcgcct
    23581 caccattgac cacacctggg tgcaggaagc ctacgaaaaa gggatcatca ctgccgagca
    23641 aatgcacgac catcccaacc tgcatgtcat ccgcaggcat ttgggcggca tcaaactgcc
    23701 cgaggtggat ttcaggttgc gtattgataa cggtgaaacg aacgaggaat ccatcaacaa
    23761 tcagggtttc caccttgccc ccggcgacac catcctcatt tgcagcgatg gcctaaccga
    23821 tctggtgtgg gacgatgaga tcctgaaaac cattcgctca aagaaagata tgaaggcctc
    23881 cgctgaagcg ctcatcaatt tagccaacga gcgcggcggc cacgataaca tcaccgttgt
    23941 catgatgacc atgccctatc tcgaagaaac gggcaaaaag aaatcaggca tcctcgattg
    24001 gctgcttggc gaagattagc aaaacggttc atgaagtttt tcttcatgaa ccgttttttt
    24061 atttttaatg gttttttggg ggaatttgcc tgcggccgct tcaaaccccg ccgaaacctg
    24121 attcaacaac gatgaacaga atctcagccc ggagaggttt atcagggctt tttgatgccg
    24181 cgttcacggt ctagccataa ctcgcggtat tttctgttcc aatcgatcaa aaatctgacc
    24241 gcgatcaccg cacccatacc cagggcggcc cacatcgaaa tatcgcggat gtttaacagc
    24301 cacaacgcag gcgcagccag aatacctgca aacacactcg cccgcgctga gtggcggata
    24361 agcaacacca gcagaagcaa caacccaaaa cagacgagga acgccaatgg gttgatcgcc
    24421 agcaaaacgc cgccagtggt tgccaacccc ataccgccgc ggaattgggc aaagacgggc
    24481 cagcagtgac cgatcaccgc aaatgaagcc gtcaacacca ggataagcgg agaagcaccc
    24541 agttgaatgg ccagataggt tgggagaaac ccctttgcta catcaagcgc aaagaccagc
    24601 gcgccccagc caaaacccgc ctgacggatg gtgttggtgg tggtggcatg ccccgagcct
    24661 gcatcacgca cgtccacttt tttgacgaag cgggtgaccc aaatggaaaa cgggagtgat
    24721 ccgcacaggt agctgaaaat ggggaggaga aaggttggca tgtgggctac gggcaaacag
    24781 gcaggtcggg gaagaattcc tctccgtatt tcaggagcag gtcgctttct tcgatgggga
    24841 aggtgagcag caccgataac aagcgcgatt tgttgagcgg ttcggcccat aagcggatct
    24901 tgtattgctg gccttcttcc acggcatcca cttcatagag caggagtcta tccttcccgc
    24961 aagaggagac aagcgtggct tcgtcgtagc cttcgaacat gatcttgagg gcgtcttcgc
    25021 tgtagaacca ggtgacatcg tcggtgccga cttcatcaca gaacatgtac acagtgacat
    25081 ccgccagcgc cgacatggtg cggtgtgtcc atgaaatggt ggattgcagc tccttgacgg
    25141 tataactgcg ctgccagttc acctcggtaa atagttcttt tccgaattcc aacccatatt
    25201 tgacgtcgtt ttctgtaggt tgcagagaag cgcagcttac gctcaggtca gatttttcca
    25261 cgctttgcga accgcaggcg ctgatgagca gggcaaaagc aaaggcgaaa ataaggacat
    25321 aaattttttt cattgatgtt tctcctcttt ggcaggtgat tccctcagtg catggcactg
    25381 ggggatgctg aaaattaatt gaacaggcgc agtgttgtgc tttttcctgg ctttgtcagc
    25441 gaggcacctt gaatggtgtg atgtctgggt caatggtttc gtcaaattca cgggcggcga
    25501 gatgtccgct gaagatggct tgggcgatga tgttgggtgc ttcggcgtcg ccaatacggc
    25561 gcagggtttt gattttgcct tcggctagtg caggttgaag ctcgtggtag agtgtgtcgt
    25621 tggggattcg gtctgtgacc atgacgatgg catcgcaggg cagggtcgtt tcggcggagg
    25681 tgatgacgtt cgtgaccgtt gcagctttcg ggtcgtagga agcgatgacg tggtttggaa
    25741 gcagggtaat gttcaagtca tgcatccgct tgaggatgcg atcctgttcg agggtgaatt
    25801 gagtccagta tgaaatgctg ggggcggggg ttatcaatgt gacttcgcag cccgcttcgg
    25861 gttgggcgag taattcggcg aggacgccgc ccatgtagta gtggtcatcg tcgtagatga
    25921 tgactttgcc tgacggtaga ttgtgattca tcaagtcgtc gggggtgaag atctttccat
    25981 tgcctgtcac ggggcgggtg aggtagcggc ccatgccatc gcgccgccag gttgcgcccg
    26041 tggctaggat gatgtgcggc gtgcccgttt caagaatatc ctgtgcggtc atggggctgg
    26101 aagggtagaa gaaaatattt tcggttttgt tgatctttgt gatgcggtaa tccaccacgc
    26161 gccgccattc gttgaggttg ggcagtgctg attcaagcag tacacgcccg cctgcttcac
    26221 gccgcgcatc ggtgaggatg acgtgatagc cgcgctgacc aagcgcgctg gcggcctcaa
    26281 gccctgctgg ccccgcgccg acgatgagga tttcatcttt gcttttcttt ggcgcaatga
    26341 tctcagggtg ccagccgcgc cgccattctt cgcccatggt tgggttttgt gtgcagcgaa
    26401 tgggaacgaa gcgtgtatcg ccagtgacgc agatgttaca gccgatgcac tcacggatgt
    26461 cttcatggcg gttttctttg atcttgttcg gcaaaaatgg gtctgcgatg ctgggacgtg
    26521 ccgcgccgat caaatccatg atgccgcgtt cgatggcgga gaccattgaa tcgggggagg
    26581 tgtagcgccc cacgccgacc acgggtttgg tggtgagctt tttgacgaag gaaatgtatt
    26641 tttcctgatg gccttctttt ccaaaacggg aggtgaccga gtcgtttgcc cagttgctga
    26701 tgttcacatc ccacaggtcg gggagttcgg cgagcatacc gacgatgtcg cgtgcttcgc
    26761 cattgtgctg cattccgtcc acacccagca attcatccac tgcaaagcgg acggcgacgg
    26821 cgcaggtatc accgacagca tcctttgtat cttcaatgag ttcgcgtaaa aaacgcacgc
    26881 ggttttcgag cgaaccaccg tattcatcgg aacggtcatt gtctttggag agcaggtgat
    26941 aggcaagggt catgttgtgc gcggcgtagc aatagacgat gtcgaagccc gcctttttcg
    27001 cgcggatggc ggcattgcga tgccatttgc ggacttgttt caggtcttct tttgaggcgg
    27061 cgcggctttg accgggttcg tatccgctgc cgccggtgat gcctgccgag cgcggcccga
    27121 aggcagcggc ccgtgaatat aggttggcag catcgtggct gttgtaggtg agttcaatgc
    27181 ctgccaatgc gccgtgtttg tgaacggctt cggtcattaa ttgccagacg gggatgtcgt
    27241 catcgctcca gatgcggcct tcgaaaaacg gagaaaggtc gctggtgtag tgaatttcag
    27301 tttcttcggt gcagaccacg ccccagccgc cttcggcttt cattccgcgc atggcggcca
    27361 tgccttcggg catccgccag ccgaagccgt tgcagtgcgg cacttgatag aaacgattcg
    27421 gcgcagtgac agggccgatc tgaatgggtt ggaagaggat atcgtaacgg gaggtcatgt
    27481 ctttttccta ggagcagttt ggcaggttgg gaaagaggcg gatggagtat tcgtccagaa
    27541 gcggttgtga ttcggctggg aatacgatca tggtgacgat gatgcggtta tctgtatcgc
    27601 tttcgaccca gtaattgatc ttgtattcga agccgaggtt gtaggttttg aattggtaca
    27661 gcctgatccc tgaatcaagt ttgcattcgg caatggattc gtagctttca tagttggcga
    27721 agatggcgct ccaggtggct tcgctgaaat cgcggttgag atcgggctct tcgtagccgc
    27781 agggtttgat ccgtgcttcg aggtaggcaa cggcaccctg cacaatgttt tgccaggtga
    27841 tggaatacgc gccttccagt gcggcttcgc tttgatccca acttgagtca aatgcatcac
    27901 ctgtgtagga gtgtgcgcgg tcaatgtctt gttgagtggg ctcaggcgag acgcagacag
    27961 gcgtaactgt tggcagcggt gtgttcgtcg ctacgattct gggtgtggat gtgatggtgg
    28021 gtggggggag cggtgtggag gtgggcggtg gtgcaggggg ctttccacag gatgaaagaa
    28081 taactgcggc aataatgagg atgaagattt gttttttcat gtttactcgt ctgggtcggc
    28141 gtttgataca gacgagtata gcacgacaaa ataggcgggg caatttattt gcccaccttg
    28201 cacagtggac ttgatacgat gcgaaattta tccgcacttt gaccgctgac catgaaccac
    28261 agacggttgt ctgtggttca gttctttgaa gcggcgtatc aactgtgctg ggtaaagtga
    28321 ggtgaaaatt tacaaacctt tatggcttta tccccgtaaa catgcgcacc caaaaatcgg
    28381 atttgaaaat gagctgctgg ttttcgagtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn gtaaacatgc gcacccaaaa atcggatttg aaaatgagct gctggttttc
    28561 gagttgggct tgatccagac gggcatttat tttgtccatg cctgcctgat aggtttcttc
    28621 actgagcagg gccagttgtg atgtggcgtt atgtttgatg aaggggtcgt ttaacacggc
    28681 cttgcccaca tgcaggtttt gaatgtgctc cacgttttgc atttgaaatc cccgaaagcc
    28741 agcctggtcc atcaggattt caattgttct ggctgaggga taacggctga ggtcggtttc
    28801 atagacggtg tcaaaatact cgtagatgta ccagctgccg tctccgctgt ggggatcgtg
    28861 gccgatgacc gccagggtgc cgccaggctt tagaatgcgg aaggcttcgt tgacaaaggc
    28921 tgcatgatcg ccaaagtgat gaatcgcatc cacgcagtag acaaggtcaa aggtctcgtt
    28981 ttgatagggg agttgggtgg ctgtgccgcg cgccagttgg atgggagcag gctgttggcg
    29041 ggcctgtgcg atcatccccg ctgaataatc catgccgtaa agctgggggg tgacctgatg
    29101 aagtagattt agccagaagc ctgtgccgct gccagcctcc agaatgcgct gtgacttggt
    29161 ccgtttggca agctccagca gggcttgtcc gcgctcccag aactgtgtgg atgggtagcg
    29221 ttggttatat tcagatgcga tcttgtcgta attgagtttt ttggtttgca tgtttttttg
    29281 agcgccccgc attgattttt cttaccatgc tacacaggca cgatctcttt cacacgcccc
    29341 acctgtccat ccatcaagcg gactttgatg ccgtgcgggt ggttgggcga tttggtgagg
    29401 atgtctttga cgacgccctt ggtgagtttg cctgtgcgtt gatcctgttt caggacgatc
    29461 agcacggtga tgcctggttt gatgttggcg cgggtttgac cgttcattgc tcatgctcca
    29521 tgggggtcat ccacgggcgg acctcgaaga tgagcagacc agatttcaca gcaggatcag
    29581 cggcggcgaa ggcttcgatc tcttcctgtg aaacctcttt ggtcgtaacc atcattccgc
    29641 ccacatcggg gaggaggaac gggccgccca attgcaattt gccttgttga tgaaacttta
    29701 gataatgttc cacatgctcg ccaacgccat cctgctggcg ataatcgatg ccgtattgcc
    29761 atttggggcc aggcttatgg atgacgacgt aacgaaccgg gcgcgtgggt gggtttgtca
    29821 tttttt
//
LOCUS       DAOQLC010000046        29616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_499_length_29616_cov_25.117351, whole genome shotgun sequence.
ACCESSION   DAOQLC010000046 DAOQLC010000000
VERSION     DAOQLC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_499_length_29616_cov_25.117351"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..941)
                     /gene="era"
                     /locus_tag="PL000_09950"
     CDS             complement(39..941)
                     /gene="era"
                     /locus_tag="PL000_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNC89266.1"
                     /translation="MTNHRSGFVAIIGRPNVGKSTLLNALLGQKVAAVSPRPQTTRKR
                     QLGILTIENAQIVFVDTPGIHNPKHMLGEFLNQEAQEALDGVDLILWLVDSSTSPTEE
                     DVQIADILSKAARRTPRMLVLNKIDMVGADDESARGEEYARLLNQATVMKVSAARRLG
                     LGEMLETLIQLLPERPPEYPEDQVTDLYEREIAGDLIREACLIRLRDEVPHGLAVRID
                     EFKERENGMAYIAATIFVERESQKGIVIGEGGRMMKTIGSQARKEIEEMGGRKVFLEL
                     RVKVSKDWRNDPNALKQLGYAKKK"
     gene            complement(938..1816)
                     /locus_tag="PL000_09955"
     CDS             complement(938..1816)
                     /locus_tag="PL000_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC89267.1"
                     /translation="MKTKHWIAFIALGVIWSSSFLWIKIGVQEVSPMVLVAFRMLFGA
                     LAASSVVIYQKLEWPRDWKTWGIYAILGPTSLAIPIFFISWGEQSIDSAVASILNATV
                     PLFTLIIAHFYLHDDRMTSQKVIGLLVGFAGIVILLSKDLTNSAHNSVIGQAAVILAS
                     LFYAGSAVWARKVTAHVQGAVRGAAPLITATIFMWILLPFMENPIKFPALPITWVAAL
                     WLGVLGSGFAMILNYYLLHEIGPTRATMVTYIFPPGGVILGVLFLDESLSWQLFAGAL
                     LIIASLAIVNWKKKES"
     gene            complement(1925..2800)
                     /locus_tag="PL000_09960"
     CDS             complement(1925..2800)
                     /locus_tag="PL000_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89268.1"
                     /translation="MKTKHWFVFITLGLIWSSSFMWIEIALREVGPLTLVAFRAFFGL
                     LFGLVVLFIQKVPFPRTWKEWAPLIALGMTNIALPFFLISWGQKTIDSGVASILDATV
                     PLFTILVAHFLVHDDKMTAQKVIGLLLGFFGVLVILIKDIGSGTNSITGEFAVILACV
                     FYAASTIIARKFTEDMPAIYRSMGPLAAATVVMIMAAAAFEAPFKIPALPLTWIALAW
                     LGILGSGLAFLIVFYLIHEIGPTRSTMVTYLMPLGGVTLGVVFLNEPLTWQMIVGAVL
                     IILSLAVANWQPKQA"
     gene            complement(3159..3578)
                     /locus_tag="PL000_09965"
     CDS             complement(3159..3578)
                     /locus_tag="PL000_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC89269.1"
                     /translation="MVTLKPKPRVTATPAADPHAANAFFSAKLTYETDPSDVYADMKN
                     ELVDFVLIDVRSAADYAKSHAQGAINLPQVSINAETTSKLPKEKLLVVYCWGPACNGA
                     TKAAAKLSALGFYVKEMIGGIEYWEDKEKYPVERGWL"
     gene            3691..4587
                     /locus_tag="PL000_09970"
     CDS             3691..4587
                     /locus_tag="PL000_09970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC89270.1"
                     /translation="MYTADMELNHLKTFVVVAEERNLTRAADRLYMTPPSVSAHIKAL
                     EDELGVILFIRKPQGMEITEHGEILRVKAEKILHSTREMLNLSKKLQVSLTGTTRIGL
                     SATSSYQRAAKLISQMQKEHPDIELTFLASCTHKIIQGLKNHSMDAGYMFGPIDDEAL
                     AGHELDIADLVIIAPSTWKDKVAGADWKEIAALPWVGSSYYCPFQTALEKLFKKRKLA
                     MNHVVWMDDEFNKCALVEEGIGMALLEKSEAEQAASNGRIFIWKTEPIKCKVSWVHLR
                     NRSDDPLIAALTAQVLNVWGKA"
     gene            4584..5042
                     /locus_tag="PL000_09975"
     CDS             4584..5042
                     /locus_tag="PL000_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89271.1"
                     /translation="MNSYLIRQAEPKDLPEIIQLCAEHAAFEQADYSPSGKADKLSAH
                     LFNNPPTCFCLVVEMDGALAGYATYSKEFSTWDADFYIHMDCLFLRPHARSMGIGEQL
                     IKQIAQSAQALNCRLIQWQTPKFNARAVKFYKRIGATEKEKLRFFLTVDA"
     gene            5139..6395
                     /locus_tag="PL000_09980"
     CDS             5139..6395
                     /locus_tag="PL000_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89272.1"
                     /translation="MEETNKNWAVRFYTLWTGQALSLLGSQLVQFAIIWYLTQTTNSA
                     TTLATASMMGLLPQVLLSPFIGTWVDRGNRRMILIFSDAIVALATLGLVILFATGQIQ
                     IWHIYIALFIRAVGGGFHQSAFGASVVLLVPKDQLARVQGFNQALNGGLNIISAPLGA
                     FLLSILPMQGILSIDVSTALLAISIVFFTHIPQPERGEKKPSTFWQDFREGFRYIMAW
                     RGLVILLGLVMVINFFFSAIEPLTPLLITKHFKGNAAQLGWWLSLFSMGVLLGGLLLG
                     IWGGFKRKIITAQGGLVLMGLFAAIIGFLPENMLWAGMVINMLFGLTQPIVNGSYGAT
                     LQAAIAPDMQGRVFSFIFSAAMLVSPLALMISGPFADRFGIQIMFVIAGVTCMLLGAA
                     GFFIPDVMDFENSSKEKSFNATVDLS"
     gene            6464..6868
                     /locus_tag="PL000_09985"
     CDS             6464..6868
                     /locus_tag="PL000_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89273.1"
                     /translation="MERKPALEPELVNEFVGKAHGDLERVKEMLENEPALVNATWDWG
                     GGDFETALGAASHMGRKDIAIFLLEHGARLDIFAAAMLGKLDIVKAVLTAYPNLLDTP
                     GPHGIPLLTHAEIGGQDAAAVSDYLQSLKRSS"
     gene            complement(6852..8513)
                     /locus_tag="PL000_09990"
     CDS             complement(6852..8513)
                     /locus_tag="PL000_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HNC89274.1"
                     /translation="MEFTEKRQHWKKINALTPQKLELAHRLLEKIREGGDVMALLRRH
                     PLEGGGYLNKAALVAAYNEMVAAGTIEPDGALLERIRMKPMRTLSGVTTVTVLTKPYP
                     CPGKCIFCPTDVRMPKSYLPDEPGAMRALEHQFDPFTQVESRIRALKNLGHPTDKIEL
                     LILGGTWSSYRRDYQEWFVKRCFDAMNNTNHGGMEPQREHEATLRSDAPVVNLPEIHS
                     QNENAPHRNVGLVIETRPDEINADEIQWLRHLGVTKVQMGAQSFNDHILDINKRGHDV
                     ESTRRAVALLRAAGFKIVLHWMPNLLGATPQSDREDFARMWEDFCPDEIKIYPNQLLA
                     NAELYEYWQRGEFHPYSTEELVDLIADIKPTIPRYCRVNRVIRDIPGNNVVEGNTRTS
                     LRQDVQEEMKKRGTRCQCIRCREVKGKAVELSSLQLNDFVYQAGRAEEHFVSFNTADD
                     GLAGFIRLSLPSKESPETGMADLNGAALIREVHVYGQSLAVGAEKTGAAQHVGLGTRL
                     LEEAEAIARANGFEKLAVIAAVGTRQYYLDRGFERGEYYLVRKIV"
     gene            complement(8547..9305)
                     /locus_tag="PL000_09995"
     CDS             complement(8547..9305)
                     /locus_tag="PL000_09995"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNC89275.1"
                     /translation="MLSAYRIEKYSQEFETKTPQEIIAWAVDRFSPYLAMSSSFQTQS
                     MPLLHMATRIHPEIPIFFIDTGYHLWETLMFREQLATEWNLKIVDLYRDSRWDVFVRQ
                     HARTLPLEDPNLCCYLHKVQPMQTALHGYKAWISGIRRDQTAVRAQAKFLELQEDGLL
                     KINPLLNWTKADVNRYIEENNLPKHPLYEKGYRSVGCAPCTIAIGVTDDERAGRWVGR
                     GKVECGLHTDMFSKKDIAEVRQDFQTDVEKVNQR"
     gene            9355..10065
                     /locus_tag="PL000_10000"
     CDS             9355..10065
                     /locus_tag="PL000_10000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89276.1"
                     /translation="MNSVTAARLIELNREFYDHFGDSFSATRQRLQPGVKKILDTIQG
                     DVSVLDLGCGNGHFLHELIRRGHKAPLLGMDFSLPLLRNAESAPGVEFREADLTKPSV
                     FISSIPSPWSMVTSFATLHHIPSNEIRLEILRTVKKLLKPDGRFVLSNWQFLNSEKLT
                     ARIQPWTRVGLSDSDVDEGDYLLDWRSGGEGLRYAHHFSAEELHGLAGQAGFKVESEF
                     LSDGENGRLGLYQIWTPD"
     gene            complement(10067..13171)
                     /locus_tag="PL000_10005"
     CDS             complement(10067..13171)
                     /locus_tag="PL000_10005"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC89277.1"
                     /translation="MQNRTEVYVSLIYILAAIPYVWLGLHAWRKRPAVAVTPFAWVML
                     GMSIWSFGYGLELFFPYLSFKLFFAKVEYIGIVSIPVLLLFFALEFTGKSHVLTPRLR
                     LLLWIFPFMMLVLVWTNEFHQLVWSMAKVVEIRNLHLLDVRFGLFFWVHIVFANLLTV
                     FASILLVRELAQRPRSDRVQIGLVILGIFLPLIGSLLFVFGLNPIPNFDITPLLFLPT
                     GLGLSWAIVRYRLQDTLPLEHLSVLNNMKDGVIVLNSKQRVLYLNPIAEKLLGCGEDT
                     AIGQPLAHVSALYWGILRPHLTAEDARAEVVIGEGRQTTVFDVTVSPIIHKDQQQRTF
                     SPDCIITLHDITERKEMENALSRREAIMSAVSMSAEQFLKEGYWGANVLAVLEKVGQA
                     VNVSRVYVAMNYKDEKNTLYSSMCYEWVSPEVVPQINNPALRHIPLQQAGLQRWEKQL
                     SQHLSIQGLVEDFPESEHAVFELLGSLSVAAMPIFVENEWWGFVVFDECRTRRHWTGM
                     ELKTLHTIASILGSAESRTRAEQKLIRRQNTLTLLHQIVQEALQADNLKAMAEDLVGR
                     LTGLISADDCYITVWDEAASQTSPLASFIASRNTFIPLQPVFGEFTLTELALNYKTTL
                     IVEDVANSPYFDREIAEQCPAQSLLAIPLVAGKNRLGAIILTFNLYHRFQAEEIEICT
                     QASNLIALAFEKFKAMEYAQRRAVTSETLRKASAVVSGTLEMDEAVTHILEQLNRVVP
                     YDSASIQLLDGNELEIVGGRGWENVENIKGMRFEIPGDNPNSVVIETKKPYILADVRK
                     AHKAFSQAPHDHIRSWLGVPLVVRDRVIGLLSIDSAEENHFNSNDIELAVTFANQVSI
                     TLENARIFKETQDQAIIDPLTTIYNRRGLYLLGESKFEDVIFGRKEFSCIMIDIDHFK
                     KINDTHGHDAGDRVLVTLASFCKKCVREIDIVGRYGGEEIVILLPETSLEVGMMVAER
                     LRAMIAGSPVKIGENFTLSVTASLGVACKDENTTSLDILIKRADQAMYIAKNKGRNRV
                     ATSK"
     gene            complement(13273..14262)
                     /locus_tag="PL000_10010"
     CDS             complement(13273..14262)
                     /locus_tag="PL000_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89278.1"
                     /translation="MTDFFSAFSKLFKSSQPKYPPDSATEPAQITQSKVLVIVFDPVM
                     DKSTGVTLSQQQKWYRPADLITGFMADLLQVSGGMARYQIVQRVDVDEFPAKTDGFRY
                     TPQTYLDVLHGVTSPHVPQEVNYNAILTNHNVLQRVARGEIDEVWTFGFPHAGFYEST
                     MGGPGAFWCNAPTLKNTEASKRRFVVMGFSYERYIGEMHESYGHRAESIMEKTFSKLT
                     GPANLWKRFIQYDKVTPGKAACGNIHFAPNSQRDYDWNNPTPVPSECYDWLLNFPNFK
                     GDVRTVGPSEWGGGDIRAHHKWWFNHFPRVAGRKNGIHNNWWQYVVNPQQVIL"
     gene            complement(14302..16752)
                     /locus_tag="PL000_10015"
     CDS             complement(14302..16752)
                     /locus_tag="PL000_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89279.1"
                     /translation="MPLDLTLAPIYRVNGQEITSLPGLIALNPPRTAARGREKDRLIV
                     YLLLTGNAVFSTSEYMKVAQDAAAAYYQSAGSLTSSLKAASEHVNKTLLERNMSSSAR
                     GQYAIAWLTLAALRDTQCTLSMSGPMHAYWFGHEDTRHIHEPAVSGKGLGASQTINIH
                     YAQLNLNAGDRLLFVGRAPDAWDSTLNDPAPSSLDAMRRRLTTLTSVDVNAVLMQATN
                     GSGNVKLLDGTLERKKEETAAPLPTPNLPQTEETVSAPQPVDAPTEDLNFEEKPENAA
                     HVVQPSAYAIPPQRTETEPANKILASLPRNTVPRDFPASIPRIQPSQQSAEVNLPPAM
                     VEEALPIETEEEKSVEEKVIAPVKAAEPETPREPSEMTRQTAKVLATGIQSTRRINQT
                     IGEKLRNFLPRLLPNSEAGDSFAPSNFSMFAIAVGVPLMVVAMLFVVYVQYGRTPQYE
                     IFLGQAREMSTQAAMLTNPIEQRESWKNVLLYVDKAEDIYRPTSDTITLREQAEVNLD
                     KLLGITRMQFNPAFSINLGIEVSRMAASETDLFLLNAANGEALRAVPASNRRGFELDT
                     AFNCKPGVYGSYTVGPLVDILTMPTLNSINATLLGVDAAGNLLYCAPGQVAQAIPLPP
                     PDTNWGRVTAFILDSGNLYVLDSSARAVWVYTGRDGTFIDRPYFFFGGQTPEKQDVID
                     LVVSGDDLYMLHADGHLSTCSYSRIESVPTRCQDPSPLINPIAAYQDTDLFGAAHFTQ
                     VLLTALPNQSILLLDADTQGVMRFTPRSLELQSQLRPTTGTLNPIPSGPVGAIAVGPN
                     HVLYLAVGGQIYFATDMP"
     gene            16803..17816
                     /locus_tag="PL000_10020"
     CDS             16803..17816
                     /locus_tag="PL000_10020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89280.1"
                     /translation="MKYTLHNDFSEINSQEWNTLLSVSITDTPFLRYEYQRSWWEHRG
                     GGEWKNAVLVLVTARENEKLIGIAPLFIAEYDGQSALLLNGSIEISDYLDLIVGQDDH
                     ARFVSGLLDFLTAQSGPANWSGLDWYNLPDSSPTLAALKAESATRGWAHHEEMYRPTP
                     RIALNGSFEEYLSRVEKKQRHEIRRKVRRAEESERGVRWSISDMADVEAEIDSFISLM
                     EQDQNKASFLHPPMRAQMRDVIRTAHQNGWLWLAFLEADGQRIAAALNFDYNNKLWGY
                     NAGVNRAYMDLSPGWVLLAYILEWCCQNGRSEFDFMRGDEEYKYRFGAVNQYVMRAKL
                     TRA"
     gene            complement(18155..18763)
                     /gene="nadD"
                     /locus_tag="PL000_10025"
     CDS             complement(18155..18763)
                     /gene="nadD"
                     /locus_tag="PL000_10025"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNC89281.1"
                     /translation="MPPTNIGIFGGTFDPPHIGHLVLASEAVQQLNLSRLLWVLAPEP
                     PHKLDQAITPLSHRLEMLKRTIADNPSFEISRVEIDRPGPHYSVDTVKLFAELYPDAE
                     LTLLLGGDSLRDLPTWRRYTELVSAVSKIGVMRRPGVSFDMPALETQIPGITQKVWFI
                     DALLQNVSSRELRRRVASGETYRYYVLPSVYDYIEANHLYGR"
     gene            18861..19142
                     /locus_tag="PL000_10030"
     CDS             18861..19142
                     /locus_tag="PL000_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89282.1"
                     /translation="MSTKNKWILGIGITLGLIVLFFLPSLWQVFFPSAGFGMMSHGNM
                     GMMNQIGSFPMRGGMGIGMSFLWFFPLVLLALIGLLMAALIKYLRSPSV"
     gene            19241..20131
                     /locus_tag="PL000_10035"
     CDS             19241..20131
                     /locus_tag="PL000_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89283.1"
                     /translation="MSNPTLISPSSPLKRIINAVHAVEAGEYVPGLLGDLINESGELG
                     QLARMLDKMAHGIAVRDHQLRLLRKVIPIGVALSAEKDYNRLLETLVVEAQFFTNADA
                     GTLYILEDNALKFVILRNTSLGMAMGGTSGNEIPFYPVKLYKADGSENRANVVSHAAL
                     THNRINVADAYEADGFDFSGTKAFDTRTKYRSKSFLAIPLTNSEDKVIGVLQLINAKD
                     PATGEIISFKDDDILEALILLATAALDGYIREASLRQEIAKLKIEINESRRSREVAEI
                     TETRYFQELKDKATQLRSRK"
     gene            20227..21792
                     /locus_tag="PL000_10040"
     CDS             20227..21792
                     /locus_tag="PL000_10040"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC89284.1"
                     /translation="MLPESPLAAIESRLRYLLPAEMYASMWTNPSVDMMIDVHKHLRT
                     LQRILHDYSSRQISSQTMKPGNIKTEWQYGTMMFTDLAGFTKLMEASSNKGREGAAEL
                     LKHLTKYFSTMIAIISKSGGDLVEFTGDALLIMFPKRDRNDDTLHAVRAGLRMQRAMK
                     EFAHIETSAGIVTLKMRIGIHSGRFLTSDIGTPRRMEHVLLGRDVQRAKLAESKGRNE
                     RVNLSPSAYERVLEHFRFEQGEKIENEKDQYYMLVEDDLADNELGEYEITPVSRRMAS
                     STLIDRDKQSVLKEITDLVNSIEPMASFLPFPVLSLLVESAAQRKIDPDFPKPTIMFI
                     NFVGLPEAADRALPGEELKLSTSFSKAFSLINAVVESRGGMLKKVTYHLSGSDIVIYF
                     GVPTAHTNDEMRAASAAVAIRDIVMNIKPPTIGNFQPEIYCMIGIHTGPAFVGEIGDP
                     RGRREYNVLGDTVNTTARLMNRAGRNQIIISESVQQAIETKYECQPLGEASLKGKSTP
                     MALYELVQKTPKP"
     gene            21897..22652
                     /locus_tag="PL000_10045"
     CDS             21897..22652
                     /locus_tag="PL000_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNC89285.1"
                     /translation="MSTIISVHSFRGGTGKSNTTANISALLAVDGARVGVVDTDIQSP
                     GIHVLFNLDESEMGHSLNDYLWGKCSIEETAHDVTSHLGAEVKGQVFLIPSSIKPGEI
                     ARILREGYDVGLLNDGFRDVVEKLKLDYLLIDTHPGLNEETLLSIAISNALVIILRPD
                     SQDYQGTAVTVDVARKLDVPKMLMLVNKVPPQFDLEDVRTRVEQTYDATVAAVLPHSD
                     EMMTLASSGIFAIRFPDHPMTKGLRKLVDQLKA"
     gene            22673..23005
                     /locus_tag="PL000_10050"
     CDS             22673..23005
                     /locus_tag="PL000_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89286.1"
                     /translation="MNETPFELAVKELEAGTEDIRPSDIVSYSEPLRSALNFVIRLGR
                     FSMTEFQEKLEFTREETKKISDILVARNLFAISRFTTAEETYFESRLTAMTRPLTRPP
                     SDIWKKIE"
     gene            23060..25105
                     /gene="ligA"
                     /locus_tag="PL000_10055"
     CDS             23060..25105
                     /gene="ligA"
                     /locus_tag="PL000_10055"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC89287.1"
                     /translation="MATKSSSRYEELKEQVNFHNYRYHVLDAPVISDLEYDRLLNELK
                     QLEADHPEWITPDSPTQRAGARPADRFEKIRHPAPILSLANAFGADDARAWFERIKKL
                     DDRVEKAKFVVEPKIDGLSVVLHYRDGMFMQGATRGDGEVGEDITSNLRTVRAIPLKI
                     PVGSEQGRQSSLHPFQVPKHLVVRGEAFIPNKDFEALNKKLEEAGEKTYLNPRNTAAG
                     SLRQLDPQLTASRPITLLVYQIVHSEGGKVPTSQWEILEYLKALGFPVTDVARRFNDL
                     EAAIAYTETFSTGRDALPYEADGMVIKIDDLTLAADLGFVGKDPRGAVAFKFPAREVT
                     TTLNDIGVAVGRTGVLTPYAMLEPVEIGGVVVERATLHNFDYIAEKDIRAGDRVLVKR
                     AGEVIPYVIGPIVDARSGKEKKYKPPSKCPACGQEVEHLEGEVAWYCVNAACPAQLVR
                     NVEHFVSRGAMDIVGLGIKIVEQLIEAGLVKDVADLFSLKKEQLLELEGFAEKKAENL
                     LGAIEQAKGQSLNRLIVALGIHGVGEVMAGDLARTFGNLSTLSKASAEELQQMDGLGP
                     NIAESIVDWFAQNANQKLLKKLKTAGVWPEMKKDEKKKEGAFTGQTFVVTGTLPTLSR
                     DGVKEFIESNGGKVTDSVSKKTSYLVLGEAPGSKLDKAKSLGVKVIDEAELRKLAEG"
     gene            25246..>26417
                     /locus_tag="PL000_10060"
     CDS             25246..>26417
                     /locus_tag="PL000_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC89288.1"
                     /translation="MDFNNTNDAVTRRIKAMTDRLDYLKSNDLYFYNQPVEEILPGNK
                     VRVRGREMGMYASYSYLGLVGHPRINEAAKKAVEKFGTGTNGVRTLAGTLTIHSELEE
                     TISNFKHTEAAITYTSGYATNLTVISTLMGRGDYVFSDKINHASIVDGCLMSGAEFRR
                     FRHNDMEHLEGLLKNAPADVAKLVIADSVFSMDGDIIDLPTMVTLCKKYNAWLMIDEA
                     HSVGVLGEKGTGIEEYFNMYGEIDIKMGTLSKTIPSVGGYVAARKEIIQYLRHASRAY
                     IFSAALPPAQAAAANEAFKVILDEPWRIERLNQNTKQFIGGLKSMGFDTLLTETAIVP
                     VLCGEDDMAFAMTRECQHNDVFVLPVVSPAVQEGLARLRATVTAAHEPSEIERAMDV"
     assembly_gap    26418..26517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26638..27735
                     /locus_tag="PL000_10065"
     CDS             26638..27735
                     /locus_tag="PL000_10065"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC89289.1"
                     /translation="MKWQWKLGKFAGIDVYVHATFLLLIGWVGYSHWLEHRNWGEVLN
                     GILFILALFVCVILHEYGHALTARKYGIKTRDITLYPIGGVARLERMPEKPIEELWVA
                     LMGPAVNVVIAAGLFAYLYLTSSLVPLNELTIASGNFLERLMTVNLSLVLFNLIPAFP
                     MDGGRVLRALLAMRMDYVRATQIAATIGQGMALLFGLVGLFGNATLLFIAFFIWIGAS
                     QESGATQMKNAISGIPVGRAMLTEYKSLSPRDTLARMSQLLLAGSQHDFPVIDGERIV
                     GVVTRDDFLMALTQHGEGIAISAVMKRDLPEVDSYEMVENALLRIQESGVPALPVTHG
                     GQLVGIVTAENIAEYLMIRSALRASRMAVPL"
     gene            27964..28635
                     /locus_tag="PL000_10070"
     CDS             27964..28635
                     /locus_tag="PL000_10070"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNC89290.1"
                     /translation="MFDQIETTILSALQTIFDQFGWAGVFGLMVFENATGITPSEIIL
                     AFAGWMLIERHGIAPTFVLIAGVYAGLGSTLGASITYWIARLGGRPVIDRFARFFRID
                     PALINKAEEQMQKWGSGLVLIGRIIPGIRTLISIPAGLAKIPFLKFFAATFIGAYIWC
                     TLLIGAGYVLGHEWMLVSEAIKQHLPLVLTLGLLAMAAVVVYQYRARIPALAWMNTKR
                     MDNES"
     gene            28632..>29616
                     /locus_tag="PL000_10075"
     CDS             28632..>29616
                     /locus_tag="PL000_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNC89291.1"
                     /translation="MNWHLLGIQESLSNLNSDQQNGLTESESSARRKQYGPNELIERG
                     GRTPWQILWAQLTATMVLILIAAAVLAGILGDYKNTISILAIVALYAILGFVQEYRAE
                     QAIAALKKMSVPNVRVLRDGSLKEVAANQLVPGDILQLETGNVVPADVRLLEAVNLRI
                     QEAALTGESEAIEKHTTALSNDPTLTLPLGDRRNMGYMGTIVTQGRGLALVIATGMQT
                     ELGKIADLIQKVEQENTPLQRRLDALGKNLALIGTGIAILILILGLLRGDELRHMLLT
                     AVSVAVAIVPEGLPAVVTITLALGAQRMLQRNALIRKLPAVETLGSVTVICS"
BASE COUNT         7282 a         7930 c         7488 g         6816 t
ORIGIN      
        1 ttcttccatt gataaagaag aaagaggaaa gatgttcgtt acttcttctt cgcatacccc
       61 aactgcttca atgcgttcgg gtcattgcgc cagtcttttg aaaccttcac acgcaattcc
      121 aaaaaaactt ttctgccgcc catctcttca atttccttgc gggcctggct gccgatggtt
      181 ttcatcattc ggccgccttc accgatgacg attccttttt gcgattcacg ttccacaaaa
      241 atggtcgcgg caatgtaagc catgccgttt tcgcgttcct tgaattcgtc aatgcgcaca
      301 gcgagtccgt gcggcacttc gtcgcgcagg cggatgaggc aggcttcacg gatcaggtct
      361 cccgcgatct cgcgttcgta caggtcggtc acttgatctt cgggatattc aggcggtcgc
      421 tcagggagaa gttggatcaa agtctcgagc atttccccaa gcccaagcct tctcgctgcg
      481 gatactttca tcaccgtggc ctgattcagc aatctggcat attcctcccc gcgagcagat
      541 tcatcatcgg cgccgaccat gtctattttg ttcagcacca gcattcgggg agtccgacgc
      601 gctgctttgc tgaggatgtc cgcaatttgg acatcctcct cggttggtga ggtggaggaa
      661 tccaccagcc agaggatcaa gtccacgcca tccagggctt cctgtgcttc ctgattgaga
      721 aattcaccga gcatgtgttt ggggttgtga atgccgggcg tatctacaaa tacgatctgg
      781 gcattctcga tggtgagaat acccagttgg cgtttgcgcg tggtctgcgg gcgcggagaa
      841 acagcggcga ccttttgccc aagcagggcg ttgagcaaag tggacttgcc cacattgggg
      901 cgtccgatga tggcgacgaa gcctgaacga tggttggtca tgattccttt ttcttccagt
      961 tgacgatggc aaggctcgcg atgatgagca acgcacccgc gaacaattgc cagcttagcg
     1021 attcatcgag gaacaacacg ccaaggataa caccacccgg cggaaaaatg tatgtcacca
     1081 tggtggcgcg ggtggggcca atttcgtgca acaggtagta attgaggatc atggcaaagc
     1141 ctgaaccaag cacacccagc cacaaggcag cgacccaggt aatgggcagt gcagggaatt
     1201 tgatggggtt ttccatgaac gggagcagga tccacatgaa gatggttgca gtgatgagcg
     1261 gagccgcacc acgtactgct ccctgcacat gagcggtgac cttgcgcgcc cacacggcgc
     1321 tccccgcata gaacagcgaa gccagaatca cagccgcctg cccgatgacc gagttgtgcg
     1381 cgctgttcgt caggtctttg ctgagcagga tgacaatgcc cgcaaacccg accagcaggc
     1441 cgatcacttt ttgtgaggtc attctgtcat catgcaggta gaagtgggca atgatgagcg
     1501 tgaacagcgg cacagtggca ttcaaaatgg aagccacagc cgaatcgatg gattgttcac
     1561 cccatgaaat gaaaaagatg ggaatggcaa ggctggtggg accaaggatg gcgtaaatgc
     1621 cccaggtctt ccaatcacgc ggccattcca gtttttgata aatcaccaca gacgatgcgg
     1681 ccagcgcgcc gaataacatg cgaaaagcga ccagcaccat ggggctgact tcctgtacgc
     1741 cgatcttgat ccacaagaag gatgaactcc agatcacgcc gagggcgatg aaggcaatcc
     1801 aatgttttgt tttcaaaata tcctgctttc tgaaattgat ggtgtatgtc gtgcgtaaac
     1861 cgaccgccga cgatggacgg caggctgcgg tcttcggtcc atgtctgatg aaattgcgga
     1921 tgttttatgc ctgtttcggc tgccagtttg cgactgcaag actcaatatg atcaacaccg
     1981 ccccgacgat catttgccag gtcagcggtt cattgaggaa gaccacgcca agggtcacgc
     2041 cgccgagcgg catgagatag gtgaccatgg tggaacgggt gggtccgatc tcgtggatga
     2101 gatagaacac gatcaggaag gccaaccctg aacctaagat tcccagccat gcgagggcga
     2161 tccacgtcag gggcagggcg gggatcttaa agggcgcttc gaacgcagca gcggccatta
     2221 tcattaccac agtggcggct gccaacggcc ccatgctgcg gtaaatggcg ggcatatcct
     2281 cggtgaattt tcgggcaatg atggtgctgg cggcatagaa tacacaggca aggatgaccg
     2341 caaattcgcc agtaattgag ttcgttccgc tgccgatgtc cttgatcaga atcaccagca
     2401 ctccgaagaa acccaataat agcccgatca ctttttgagc cgtcattttg tcatcatgca
     2461 cgaggaagtg tgcaacgaga atggtaaaca gcggaacggt cgcatccaaa atggaggcca
     2521 caccagaatc gatggttttt tgtccccagc tgatcagaaa gaacggcagg gcaatattgg
     2581 tcatgccaag tgcgatcagc ggcgcccatt ccttccatgt gcggggaaaa ggcacctttt
     2641 gaataaaaag gaccaccaac ccgaacaaaa gcccaaagaa ggcgcgaaag gcaacaagcg
     2701 taagcggtcc cacttcgcgc agggcaattt cgatccacat aaaggatgaa ctccagatca
     2761 aaccgagggt gataaagaca aaccagtgtt ttgttttcaa tgtaactcct taaaatacgt
     2821 caggcttttt tgcagcttgt acgtgtgaat acgaatttta taggaaggtt gtctttatca
     2881 gccacccgat tcttgctctt ttagggatcg atgtaacctt ccggcttata aaccctgatc
     2941 ttggaggctg ggttctcttt tagcctggat gccgggcggc gtggccgctg tacaagctcg
     3001 ccgccgcagt ttgggcaaat gttatttagt ttggaatcag cgcaatgggc acaaaaggta
     3061 cattcaaagg tgcagatgcg cgcctctgtt gaatcaggcg ggaggtcaat gttgcagcat
     3121 tcacagccgg gtctcatttc caacatggat tctccttttt agagccaacc gcgttccacg
     3181 gggtattttt ctttgtcttc ccaatattca atgccgccga tcatctcttt gacgtaaaag
     3241 ccaagagcgc tgagtttggc tgcggctttg gttgcgccgt tgcaggctgg cccccagcaa
     3301 tacacgacca gcaacttttc tttgggcagt ttggatgttg tttcggcatt aatacttacc
     3361 tgaggcaggt tgatggctcc ctgggcatgg cttttggcat agtcggctgc gctgcgcaca
     3421 tcgatcaaaa caaaatccac caactcattc ttcatatccg cataaacatc tgaagggtcg
     3481 gtctcatagg tcagcttggc ggaaaagaaa gcatttgctg cgtgcgggtc tgctgcaggg
     3541 gtggctgtaa ctcttggttt gggtttaagt gtgaccattt tttctccttg aattggaatg
     3601 ttccaagtgt atggaaaaga tgcctgtttg tgaatttgag gttgccaaac acagcgttcg
     3661 gtgaagcgaa acgctcggca tgacagaatc atgtatactg cggacatgga actgaatcac
     3721 ctgaagacat ttgttgttgt agctgaagaa agaaacctga cgcgcgcagc cgacagactt
     3781 tatatgacac cgccttctgt cagcgctcac atcaaggcat tggaagatga gttgggcgtg
     3841 atcctcttta tccgtaaacc gcaaggcatg gaaattacgg agcatggaga aatcctgcgc
     3901 gttaaagccg agaagatcct gcactccaca cgtgagatgc tcaatttgtc gaaaaagctg
     3961 caagtctctc tcacaggcac aacccgcatc ggattaagcg ccacatcatc ctatcaacgt
     4021 gccgcaaaac tcatcagtca gatgcaaaaa gaacatccag atatcgagtt gacctttctt
     4081 gcgtcctgca ctcacaagat cattcaggga ttgaagaatc attccatgga tgcagggtat
     4141 atgtttggcc ccatcgacga tgaagccctg gctggacacg aattggatat tgccgacctt
     4201 gtgatcattg ccccctcaac ctggaaggat aaggtggcgg gggcagactg gaaagagatc
     4261 gcggcattgc cctgggttgg gtcatcttat tattgtccct tccaaaccgc gcttgaaaaa
     4321 cttttcaaaa agagaaaact cgccatgaac catgtggtct ggatggatga tgagttcaac
     4381 aagtgcgcac tcgtggagga aggcattggc atggcattgc tcgaaaaaag tgaagcggaa
     4441 caggctgctt caaacggcag gatatttatc tggaaaactg aaccgatcaa atgcaaggtc
     4501 tcgtgggtgc atttacgaaa tcgcagcgat gaccctttga ttgccgccct gacggctcaa
     4561 gtattgaatg tatggggaaa agcatgaatt cgtatttgat acgacaggca gaacccaaag
     4621 acctgccaga gatcatccag ctatgcgctg aacatgccgc atttgaacag gcggattatt
     4681 ctccatctgg caaagcggat aaattatcgg cgcatctctt taacaacccg ccaacctgtt
     4741 tctgtctcgt cgttgaaatg gacggcgccc tggcagggta cgccacctat tccaaagaat
     4801 tctccacatg ggatgcagat ttttacatcc acatggattg cctgttcctg cgtccgcacg
     4861 cccgcagcat gggaattggg gaacaattga tcaagcagat cgcacagtca gcacaggcat
     4921 tgaattgccg cctcatccag tggcagaccc ccaaattcaa cgcacgggcg gtcaaattct
     4981 acaaacgcat cggcgctact gaaaaggaaa agcttcgttt tttcctgaca gtggatgcgt
     5041 aaagatcatc aacaaaacag aataaccatt ctcaaggggg ttatcataaa agcgcgacca
     5101 tactaccatt gagggaaaaa tctcttagag gtgtcttcat ggaagaaaca aacaaaaatt
     5161 gggccgtccg tttttatacc ctgtggacag ggcaggcgct gtccctgctt ggcagccaat
     5221 tggtgcagtt cgccatcatc tggtacctca cgcaaacgac gaactcagcc accaccctcg
     5281 caactgcttc aatgatgggg ttgcttccgc aggtgctact ctcccccttc atcggcacct
     5341 gggtggatcg cggcaatcgc cgcatgatcc tcatattttc agatgcgatc gttgcccttg
     5401 caacgcttgg actggtcatt ttatttgcca cagggcagat ccagatttgg catatttaca
     5461 tcgccctgtt catccgcgct gtaggaggcg gatttcatca atcggcgttt ggcgcatcgg
     5521 tggtattgct cgtgcccaaa gatcaacttg cgcgtgtgca gggattcaat caggcactga
     5581 acggcggttt gaatatcatc tcagcgccgc tgggggcttt tctgctttcc atactgccca
     5641 tgcagggcat tctttccatt gacgtgagca cggcgctgct tgcaatttcg atcgtcttct
     5701 ttacccacat cccacagccg gagcgtggcg agaaaaagcc ctctaccttc tggcaggatt
     5761 ttcgggaagg ctttcgctac atcatggcct ggcgcggatt ggtcattctg ttggggctgg
     5821 taatggttat caactttttc ttcagcgcca tcgaaccgct gaccccattg cttatcacaa
     5881 agcatttcaa aggcaatgcc gctcaactgg gctggtggct ttccctgttt tctatggggg
     5941 tcttgctggg cggtttgttg ctggggattt ggggaggctt caaacgcaag atcatcaccg
     6001 cacaaggcgg gttggttcta atgggtttat ttgccgccat tatcggcttc ctccctgaaa
     6061 atatgctatg ggctggcatg gtcatcaata tgctgttcgg gttaacccaa cccattgtca
     6121 acggttcgta tggcgcgacc ctgcaggcag ccatcgcacc tgatatgcaa ggcagggtgt
     6181 tctccttcat ctttagcgcg gccatgctgg tctcaccgct ggcgctcatg atctcaggtc
     6241 catttgccga ccgcttcggg atccagatca tgttcgtgat cgcaggagtc acttgcatgc
     6301 tcctgggagc cgcgggcttc ttcatccccg atgtgatgga tttcgaaaat tcttcaaagg
     6361 agaaaagctt taatgccacg gttgatcttt cctgattttg attcacttcg ggatcaggag
     6421 tagaatttcc attcaaaaga acatttatca acaggagcaa acaatggaac gaaaaccagc
     6481 tttagaaccc gaacttgtga atgaatttgt cggcaaggca cacggcgacc ttgaacgtgt
     6541 caaagagatg ttggaaaatg agccagcatt ggtcaatgca acatgggatt ggggcggggg
     6601 tgattttgaa accgcgcttg gtgcagcttc gcacatggga cgcaaagaca tcgccatctt
     6661 tttgctcgaa catggtgcgc gccttgatat tttcgcagca gccatgcttg gaaaactcga
     6721 tatcgtcaaa gccgtgctga cagcctaccc caacctgcta gacacccccg gtccacacgg
     6781 aattccgctc ctcacccatg ccgagatcgg cggacaggat gcggcggcag tttcggatta
     6841 tttacaatcg ctcaaacgat cttcctgacg agataatatt ccccgcgctc aaacccgcga
     6901 tcaagataat attgccgcgt gccaactgcc gcaatgacgg ccagtttctc aaaaccgttg
     6961 gcgcgcgcaa tggcttccgc ttcttcgagc aggcgtgtac ccagcccaac atgctgggcg
     7021 gcgcctgttt tttctgcgcc caccgccagt gactgaccgt acacatgcac ttcgcgaatc
     7081 aaggccgcac cattgaggtc agccatgcca gtttcagggg attcctttga aggcagcgaa
     7141 aggcggataa atcccgccag cccatcatcg gctgtgttga acgagacgaa gtgttcctct
     7201 gccctgccag cctgatatac aaaatcattt aattgcagcg acgataattc gaccgccttg
     7261 cctttcacct cgcggcagcg aatacactga caacgcgtgc cgcgcttctt catctcttcc
     7321 tgtacatcct gccgcaaact ggtgcgcgta ttgccttcga caacgttatt ccccggtata
     7381 tcgcggatga cgcgattgac acggcaataa cgcggaatgg tgggtttgat gtcggcgatc
     7441 agatccacaa gctcttcggt tgagtaggga tgaaactcgc cgcgctgcca gtattcatac
     7501 aactcggcat tggccagcaa ttgattcgga tagatcttga tctcatcggg gcaaaaatcc
     7561 tcccacatcc gcgcgaaatc ttcgcggtcg ctttgcgggg tggcgccgag caggttgggc
     7621 atccaatgca gcacgatctt gaatcccgct gcgcgcagca atgccaccgc cctgcgcgtg
     7681 gattcgacat catgtccgcg tttgttgatg tcgaggatgt gatcgttgaa actttgcgcg
     7741 cccatctgca ccttggtcac gccaagatgc cgcagccatt gaatttcatc ggcattgatc
     7801 tcatcggggc gggtttcgat caccagcccc acattgcgat gcggggcatt ttcattttgg
     7861 gagtgaattt caggcagatt aacaacaggc gcgtcggaac gcagagttgc ttcgtgttct
     7921 ctctgcggct ccatgcctcc gtggttggtg ttattcatcg cgtcgaagca acgtttgacg
     7981 aaccattcct gataatcgcg tcggtacgat gaccacgtgc cgcccaggat caacaactcg
     8041 atcttatcgg tggggtgacc gagatttttc aaagcgcgga ttcgtgactc aacctgtgtg
     8101 aacgggtcaa attgatgttc cagtgcgcgc attgccccgg gctcatcggg caaatagctt
     8161 tttggcatac gcacatcggt ggggcaaaag atgcacttcc ccgggcaggg atacggcttc
     8221 gtcagcacgg tcacagtcgt cacgcccgaa agcgtgcgca tgggcttcat gcggatgcgt
     8281 tccagcaatg caccatcggg ttcaatggtg cctgctgcga ccatttcgtt atacgcggcc
     8341 accaacgcag ccttgttcaa atatccgcca ccctccaacg gatgacggcg cagcaaagcc
     8401 atcacgtcac cgccttcgcg gatcttttcc aacaggcgat gagccaactc cagtttttgc
     8461 ggagtcagcg cgttgatctt tttccagtgt tgtcgttttt cagtaaattc catggtttcc
     8521 acgcaggagc tgcccagaga caaaaactat ctctggttca ccttctctac atccgtctga
     8581 aaatcctgcc gcacttcggc aatatccttc tttgagaaca tatccgtatg caggccgcat
     8641 tccacttttc cgcgcccaac ccatcgaccg gcacgttcat cgtccgttac gccaatggca
     8701 atcgtgcagg gggcgcaccc cacactgcgg tatccttttt catacaatgg atgtttcggc
     8761 aggttgtttt cttcaatata acgattcaca tcagccttcg tccaattcaa cagcggattg
     8821 atcttcaaca atccatcttc ctgtaattcc aaaaactttg cctgcgcccg caccgcggtc
     8881 tggtcacggc ggatgccgct gatccaggct ttgtagccgt gtaaggccgt ctgcatcggt
     8941 tgaactttgt gcaaataaca gcacaggttc gggtcttcaa gcggcagggt ccgtgcgtgc
     9001 tggcgcacaa acacatccca ccgcgaatcg cgatacagat ccacgatctt taaattccat
     9061 tcagtggcaa gctgctcacg aaacatcaag gtttcccaaa gatgataacc tgtgtcaatg
     9121 aagaagatcg gaatctcagg gtggatacgc gtcgccatgt gcagcaacgg catagactgg
     9181 gtctggaagg atgaactcat cgcaagatac ggcgaaaatc gatccacagc ccaggcaatg
     9241 atctcctgcg gggttttggt ctcgaattcc tgtgaatact tctcaattcg ataagccgat
     9301 aacatactgg gattataccg tttatataat gcccactctg ggtataatgc cgccatgaat
     9361 tctgtcactg ccgcacgcct gatcgaactt aaccgcgaat tttacgacca cttcggcgat
     9421 tcattttccg ccacacggca acgcctgcag cccggcgtaa aaaagattct cgatacgatt
     9481 cagggggatg tctcagtatt ggatctggga tgcggcaacg ggcattttct ccacgagttg
     9541 attcggcgtg gtcacaaagc tccgcttctc ggcatggact ttagcctgcc cctgctccga
     9601 aacgcagaat cagcgccggg ggtggagttt cgagaggctg atcttacaaa gccatccgtg
     9661 ttcatttcat ccatcccttc tccgtggtcc atggtcacct cctttgccac cctgcaccac
     9721 atcccttcca atgaaatccg cctcgaaatc ctgcgaaccg tgaaaaagct gctcaagccc
     9781 gatggcaggt tcgttttatc caactggcaa tttctaaaca gcgaaaaact caccgcccgc
     9841 atccaaccgt ggacacgggt gggcctgtcc gattcagatg tagatgaggg ggattacctg
     9901 ctcgattggc gcagcggcgg cgagggcctg cgatacgcgc accatttttc agcggaggaa
     9961 ctgcatgggt tggctgggca ggctgggttt aaggtggaat cggaattttt atccgatggt
    10021 gaaaatggca ggctgggact gtaccaaatc tggaccccag actgaatcat ttgctggttg
    10081 ccacgcggtt gcgccccttg tttttggcaa tgtacatcgc ctggtctgcg cgtttgatga
    10141 gaatatcaag ggacgtggtg ttttcatcct tgcaggccac ccccaaactc gcagtaactg
    10201 acagggtgaa attttcaccg atcttgaccg gagaacccgc gatcatggcg cgcaggcgtt
    10261 ctgccaccat catccccact tcaaggctgg tttcgggcag caaaatgacg atctcttcgc
    10321 cgccgtaacg gccaacgata tcgatctcac gcacacattt tttacagaag gaagccagtg
    10381 tcacaagtac cctgtcgcct gcgtcgtggc cgtgggtatc gttgatcttt ttgaaatggt
    10441 caatgtcgat catgatgcag gaaaattcct tcctgccaaa aatcacatct tcaaatttac
    10501 tttcgcccaa aagatataaa ccgcggcggt tgtaaatggt ggtgagcgga tcaattatgg
    10561 cctgatcctg ggtctctttg aagatgcggg cattttccag cgtgatcgaa acttgattgg
    10621 caaaggttac agcgagttca atatcattac tgttgaagtg attctcctcg gcactgtcaa
    10681 tggaaagcag gccgatgacc ctgtcacgca caaccagcgg cacccccaac caggagcgga
    10741 tgtgatcatg cggcgcctgg ctgaaagcct tgtgcgcttt tcgcacatcg gcaaggatat
    10801 atggtttttt ggtttcgatc accacactat tcgggttatc gccgggaatc tcaaacctca
    10861 tccccttgat attttccaca ttctcccagc cgcgcccgcc cacgatctcc aactcgttcc
    10921 catccagtaa ttgaatggat gcgctatcgt acggcacgac ccgattcaat tgttcgagga
    10981 tatgggtgac cgcctcatcc atttcaagtg tgccagaaac aacggcactg gctttgcgca
    11041 gggtttcaga agttaccgca cggcgctggg cgtattccat ggctttgaat ttctcgaacg
    11101 ccagcgcgat caaattggaa gcctgggtac aaatctcgat ctcttccgct tgaaaccgat
    11161 gatacaagtt aaaggtcagg atgatggcgc caagtctatt cttcccggcc accaatggta
    11221 tggccagcaa agattgcgca gggcattgtt ctgcgatctc acggtcaaaa tatggagagt
    11281 tcgccacatc ctccacgatc agggtcgttt tgtagttcaa ggccagttcg gtcagtgtga
    11341 attcgccgaa tacaggttga agcggaatga aggtattacg ggaagcaata aaagaagcca
    11401 gcggagaagt ttgtgaagcg gcctcatccc ataccgtaat gtaacaatca tctgcagaga
    11461 tcaaccccgt cagccgcccc accaggtctt cagccatggc ctttaagtta tcagcctgca
    11521 atgcctcctg tacgatttga tgcagcaagg tcagcgtatt ctgcctgcga atcaattttt
    11581 gctcggcacg ggtacgcgac tcggcagacc ccaaaatgct ggcaatggtg tgcagggttt
    11641 ttaattccat gcccgtccaa tgcctgcggg tgcggcattc atcgaacaca acaaatcccc
    11701 accactcgtt ctccacaaaa atgggcatgg ccgccacaga caggctgcct aataattcga
    11761 aaaccgcatg ctcagattct ggaaaatcct ccaccagtcc ttggatcgac aaatgctgtg
    11821 aaagttgttt ttcccaacgc tgcaacccag cctgttgaag tgggatatgc cgcaaggcag
    11881 ggttgttgat ctgcggtaca acctcggggg aaacccattc atagcacatg ctggaataca
    11941 gggtgttctt ctcgtccttg taattcattg ccacataaac gcggctgaca ttcaccgcct
    12001 gccccacctt ttccagcaca gccaaaacat tggcacccca ataaccttcc ttcaaaaatt
    12061 gttccgccga catgctcacc gccgacatga tcgcctcacg gcggctgagc gcattttcca
    12121 tttccttacg ctcggtgatg tcgtgcagtg tgatgatgca atcaggtgaa aatgttctct
    12181 gctgctgatc cttgtggatg atgggagaaa cggtcacatc aaaaacggtg gtctgcctgc
    12241 cctcgccgat cacaacctca gcccgggcat cttccgccgt caaatgcgga cgcaggatgc
    12301 cccaatacaa cgcagagaca tgcgcaagcg gctgcccaat ggcagtgtct tcgccgcatc
    12361 ccaacaactt ctctgcaatc ggattcaaat acaaaacacg ctgttttgaa ttcaagacga
    12421 tcacaccgtc tttcatgtta tttaaaacgc ttaagtgctc aagcggcagg gtatcttgca
    12481 aacggtaccg tacaatcgcc catgaaagcc caagcccggt tggcaggaac aacaacggcg
    12541 ttatatcaaa attgggtatc ggattcaagc caaacacaaa aagcaggctg ccgatcaacg
    12601 gcaggaaaat tcccaaaatc accaacccaa tctgtacccg atccgatctt ggacgctggg
    12661 ctaattccct gaccagcaga atactggcaa ataccgtcag taaattcgca aacacaatat
    12721 gcacccaaaa gaacaaccca aagcggacat ccaacaagtg caggttacga atttccacca
    12781 ccttcgccat ggaccagaca agttggtgaa actcattggt ccatacaaga accagcatca
    12841 tgaatgggaa tatccacaac aacaggcgca gacgcggggt gagtacatga ctcttgccgg
    12901 taaactccaa agcaaagaac aataacaata ccggaatgga aacaatcccg atgtattcga
    12961 cttttgcaaa aaacaattta aaagaaaggt acgggaagaa aagctccaaa ccatacccaa
    13021 atgaccagat ggacataccc agcatgaccc aggcaaacgg ggtcaccgcc actgcagggc
    13081 gtttacgcca tgcatgcaaa cccagccaga catatggaat tgcggccaga atatatatta
    13141 atgagacata gacttcggtg cgattctgca tacttactaa aaaacacatc actaaaggct
    13201 gtgatacgcc ctttcgccac ttttaaaaac cccgcgcacc ttgatatgtc acagtgcgcg
    13261 gagaagagtt gtctataaga ttacctgctg gggattaacc acatattgcc accagttatt
    13321 gtggatccca ttttttctcc ctgccactcg tggaaaatga ttgaaccacc atttatgatg
    13381 ggctcgaata tcgccgccgc cccactctga gggccccact gtgcgcacgt cacctttgaa
    13441 attggggaaa ttcaaaagcc agtcataaca ttcgcttggc accggggtgg ggttgttcca
    13501 atcgtaatcc ctttggctgt tgggcgcaaa atggatgttt ccacaagcag ccttgcccgg
    13561 cgtaaccttg tcatattgaa taaaacgctt ccacaaattg gctggaccgg tcaacttcga
    13621 aaaggtcttt tccatgatcg attcagcacg gtgaccataa gattcgtgca tttcgccgat
    13681 gtatctttca taagaaaagc ccatcaccac aaagcggcgt ttgctagcct ctgtattttt
    13741 caacgtcggg gcgttgcacc aaaatgcacc ggggccgccc atcgtcgact cataaaaacc
    13801 tgcatgtgga aagccgaagg tccacacctc atcaatctca ccacgggcca cccgctgcaa
    13861 cacattgtga ttggtaagaa tggcgttgta attcacttcc tgcggcacat gtggagaggt
    13921 aacaccatgc aacacatcca ggtaggtttg cggagtatag cgaaacccat cggtcttggc
    13981 agggaactca tccacatcca cccgttgaac gatctgatac cgcgccatcc ccccgctgac
    14041 ctggagcaga tcggccataa accctgtaat gaggtcggca gggcgatacc atttttgctg
    14101 ctgcgaaagc gtcacccctg tggatttatc catcacagga tcgaacacga tcaccagcac
    14161 cttactctgg gtgatctgcg cgggttccgt ggcgctatca ggcggatact tgggttggga
    14221 agatttaaaa agtttggaga atgcgctgaa aaaatccgtc ataatttctc ctgtataggg
    14281 ttgaagatcg gcagcaaagt tttacggcat gtcggttgcg aaatagatct gcccgcccac
    14341 agccagataa agcacatgat tgggaccaac cgcaatcgcc cccacaggcc cggagggaat
    14401 cggattcaaa gttcctgttg taggccgcaa ttgactctga agctcaagtg agcgcggcgt
    14461 aaaccgcatg acaccctgcg tatcggcatc cagcaacagg atggattgat tcggcaaagc
    14521 cgtcagcagg acctgtgtaa agtgagcggc tccaaataaa tctgtatcct gatacgctgc
    14581 gatcgggttg atcagcggcg acggatcctg gcagcgcgtg ggcaccgact cgatgcggct
    14641 gtacgaacag gtggaaagat gcccatcggc atgtaacatg tacaagtcat cacctgaaac
    14701 gacaaggtca attacatcct gtttttcagg ggtctgcccg ccaaagaaga aatatggacg
    14761 atcaatgaag gtgccatctc tgcctgtgta aacccacaca gcacgcgccg aagaatcgag
    14821 cacgtacaaa ttgccgctat ccaaaatgaa ggcggtcaca cggccccagt tcgtatctgg
    14881 cggcggcaat ggaatggcct gtgccacctg tcctggcgcg caatacaaaa gattcccagc
    14941 cgcatccaca ccgagcaagg ttgcattgat cgagttaagc gtgggcatgg tcaaaatatc
    15001 caccagcgga ccgaccgtat aactgccata cacaccaggc ttgcaattga aagcggtatc
    15061 caattcaaag ccgcggcggt tactggctgg cacggcacgg agcgcctctc cattggccgc
    15121 attcaacaaa aacaaatctg tttcgctggc agccatgcgg ctgacctcga tgccgaggtt
    15181 aatgctgaaa gcagggttga actgcatccg cgtgatgccc agcaatttat cgagattgac
    15241 ttcagcctgc tcacgcaagg tgatggtatc ggatgtggga cgataaatat cttcagcctt
    15301 atccacgtac aacaaaacat tcttccagga ttcgcgctgt tcgatgggat tcgtcagcat
    15361 ggcggcctgc gtgctcattt cacgcgcctg cccgaggaaa atctcatatt gcggcgtacg
    15421 tccatattgg acatacacca cgaaaagcat cgccaccacc atcaatggca cgcccaccgc
    15481 aatggcaaac atggaaaagt tggaaggcgc aaaactatca cccgcctctg aattcggcag
    15541 caagcgtgga aggaagttcc gtaacttctc gccgatggtc tgattgatgc ggcgggtgga
    15601 ctggatgccc gttgccagaa cttttgctgt ctggcgggtc atctcagagg gttcgcgggg
    15661 cgtttcaggc tcggcggcct ttacaggcgc aatgaccttt tcctcaacac ttttttcttc
    15721 ctctgtctca atgggcaaag cctcttccac catggcaggg ggaaggttta cctcagcact
    15781 ttgctgtgac ggctgaattc tgggaatgga ggcagggaaa tcacgcggga cggtattccg
    15841 cggcaggctg gccaagatct tattggcagg ttctgtttcg gttctctgcg gcggaatggc
    15901 atatgcggaa ggttgaacaa catgcgctgc gttttcgggc ttctcttcaa aatttagatc
    15961 ttcagttggg gcatccacag gttgaggtgc agaaactgtt tcttcggtct gtggcaggtt
    16021 cggggtaggc agaggcgcag ccgtctcttc cttttttcgt tcgagtgtcc cgtctaaaag
    16081 ttttacattc cctgaaccat tcgtggcctg catcaatacg gcgttcacat ccacactggt
    16141 aagggtggtc agccttcgac gcatggcatc cagggaagaa ggggcgggat cattgagcgt
    16201 gctatcccag gcatcgggcg cacgtcctac aaacaacagg cgatcacctg cattgaggtt
    16261 gagttgggca tagtgaatat tgatggtctg gctggcaccc aaccctttgc cagaaacagc
    16321 aggctcatga atatggcgcg tatcttcgtg accgaaccag tatgcatgca tggggccgct
    16381 catcgaaagc gtgcattgcg tatcgcgcaa tgcggccagg gtcagccagg caatggcgta
    16441 ttgtccgcgc gcgctggatg acatgttgcg ttcaagcagg gtcttgttca catgctcaga
    16501 agcagctttc agtgaactgg taagcgaccc cgcagattga tagtaagccg cagcggcatc
    16561 ttgcgccacc ttcatatatt cgctggtcga gaagacagca ttccccgtca aaagcagata
    16621 aacgatcaga cggtcctttt cacggccccg cgccgcagta cgcggcgggt tgagcgcaat
    16681 caaacctggc aggctggtaa tttcctgtcc attcacccga tagatgggag caagagtcaa
    16741 atcaagtggc atgtaagaat agttcctaat ccgctatcat tatactggta aaatttgcgc
    16801 ccatgaaata cacactccat aacgattttt cagagatcaa ttcacaggaa tggaacacat
    16861 tgttgtcggt ttcgataacc gacactccat ttttgcggta cgaatatcag cgttcatggt
    16921 gggagcaccg cggcggcggc gagtggaaaa atgccgtcct ggtgctggtc accgcacgcg
    16981 aaaatgaaaa gctcattggc attgcccctc ttttcatcgc cgaatacgac ggacaatctg
    17041 cgcttctctt aaatggaagc attgaaatct ccgattatct tgacctgatc gtcgggcagg
    17101 atgaccatgc ccgcttcgtt tcaggtctgc tggattttct aacggctcaa agcggacccg
    17161 ccaactggag cggcctcgat tggtataacc tccccgactc atcccccacc cttgccgccc
    17221 tcaaagcaga atctgcaact cgcggctggg cacatcacga ggaaatgtac cgccccacac
    17281 cccgcattgc cttgaacggc agtttcgaag aatatctttc acgtgtggaa aaaaagcagc
    17341 gccatgaaat ccgccgtaaa gtgcgccgcg ccgaagaaag tgaacgtggg gttcgctggt
    17401 ccatttcaga tatggccgat gtggaagctg aaatcgattc attcatcagc ctgatggagc
    17461 aggaccaaaa caaggcaagt ttcctgcatc cgccgatgcg cgcccaaatg cgcgatgtca
    17521 tccgcaccgc ccaccaaaat ggctggctct ggctggcctt cctcgaggcg gatggtcaac
    17581 gtattgcagc ggcgctcaac tttgattaca acaataaatt atgggggtac aacgcaggcg
    17641 tcaaccgcgc ctacatggac ctttcccccg gctgggtcct gctggcttat attttggagt
    17701 ggtgctgtca aaacggacgc agtgaattcg atttcatgcg cggtgacgaa gaatacaaat
    17761 accgcttcgg cgccgtgaac caatacgtga tgcgggcaaa actcacacgg gcttaaaccg
    17821 caaaacatga ttcactccaa acgtaaggaa tcagttgcga aatacgcggt tcgtcgcgaa
    17881 tttttgcaaa agtctgaaag attaaccacg gagacacaga gaaaaaaata aaaactccgc
    17941 ctgcaggagg ctccgcactg actcagtgtc tctgtgcttc aaaatggctt ttcaaagata
    18001 ttccctaaac cttacgcaag cagtcaaatg aattgcaaga tacggacgag ttataaaaaa
    18061 gccgaccctg taaaatggat cggctttttt cgtgtaaatt cgcgaaacct gcggctaaag
    18121 aattgagtgt ttttgaagat caacagagaa agaactatct tccataaaga tggttcgctt
    18181 caatataatc atacacagag ggaagtacat aataccgata ggtttccccg cttgcaacac
    18241 ggcggcgaag ttcacgcgaa gatacatttt gcagcaaagc atcaatgaac cagacctttt
    18301 gcgttatccc gggaatttga gtttccaaag caggcatgtc aaaagagacg cccgggcggc
    18361 gcatcacacc aattttggaa acagcagaca ccagttcagt atagcggcgc caggtcggca
    18421 ggtcacgcag ggaatcaccg ccgagcaaaa gggtgagttc cgcatcgggg tatagttcgg
    18481 caaacagttt cacagtatcc acactgtaat gggggccagg gcggtcaatt tcaacacgtg
    18541 agatttcaaa agacggatta tctgcaatgg tccgcttcaa catttcaagg cggtgtgaaa
    18601 gcggcgtaat ggcctggtcc aatttatgcg gaggctcggg cgcaagcacc cataacaggc
    18661 gggaaaggtt aagctgctga acagcctcac tggcaagaac aaggtgaccg atatgcggcg
    18721 gatcaaaggt accgccaaag attccgatgt ttgtaggtgg catgttgaaa gtatatctca
    18781 aagttttaat ggatgttttt gttttcttta cagtcccttt atattaggcc gctatgataa
    18841 agacaataaa ggagatcact atgtctacaa aaaacaaatg gattcttggc atcggcatca
    18901 cacttgggct gattgtgttg ttcttcctcc cttcgctctg gcaggtcttt tttccgtccg
    18961 ctggctttgg catgatgagc cacggcaata tgggtatgat gaatcaaatt ggctctttcc
    19021 caatgcgtgg aggaatgggg ataggcatgt ccttcctctg gttcttcccg cttgtattgc
    19081 ttgccctgat cggtctgctc atggcggctt tgatcaaata tttgcgaagc ccctctgtgt
    19141 aaccccatag caagataatc aaccagcccc gtcttttaga cggggctggt ccatgttaca
    19201 gagaaagctg gtggtatact tttgcaaccg caggaaaaac atgtcaaacc ccaccttaat
    19261 cagcccatcc tccccattaa agagaattat caacgctgtc catgcagtgg aggcgggtga
    19321 gtatgtgcca ggcctgcttg gcgacctgat taacgagagc ggtgaacttg gtcaactggc
    19381 ccgcatgctc gataaaatgg cacatggcat tgcggtgcga gaccaccagc tgcgtttgct
    19441 ccgcaaagtg atcccgatcg gggtggccct atcggctgaa aaagactaca accgcctgct
    19501 tgaaaccctg gttgtggagg ctcaattctt caccaacgcc gatgcgggca cgttgtacat
    19561 tctcgaagac aacgcgctca aatttgtgat cctgagaaac acctcgcttg ggatggccat
    19621 gggtggaacc agcggcaatg agattccttt ttacccggtg aaactataca aagccgatgg
    19681 cagtgagaat cgggcgaacg tggtgtcgca tgctgcactt acccacaacc gcattaatgt
    19741 cgctgatgcc tatgaagccg atggcttcga cttctccggg acgaaagcct tcgatacccg
    19801 cacaaaatac cgctccaaat ccttcctcgc aattccactc acaaattcag aagacaaggt
    19861 gatcggtgtt ctgcaattga tcaacgccaa ggatcccgcc accggtgaga tcatctcctt
    19921 caaagatgac gacatccttg aagccttgat cctgcttgca accgccgcat tggacggtta
    19981 tatccgtgaa gcgtctctgc ggcaggagat cgccaaatta aagatcgaga tcaacgaatc
    20041 gcgccgctcg cgcgaggtgg ctgaaattac agagacacgc tattttcagg aattaaaaga
    20101 taaagccacc cagttgcgca gccgaaaata gatctggttt tatttttata accagtacaa
    20161 atggagcggg gctatttcaa tgtcactaca taatttgaga catgaaatca agttataatt
    20221 ttcaacatgc tgccagaatc gccgcttgcc gccattgaaa gccgattaag atacctgctc
    20281 ccggcggaga tgtatgcctc catgtggacc aatccctcgg ttgacatgat gatcgacgta
    20341 cacaagcacc tgcggacgct ccaacgcatt ctgcacgact actcatcacg acagatctca
    20401 tctcaaacga tgaaacctgg aaacatcaaa accgaatggc agtatggcac catgatgttc
    20461 accgatctgg caggctttac caaattgatg gaagccagtt caaacaaggg acgcgaaggc
    20521 gctgccgaac tcctgaaaca cctgacaaaa tatttctcca caatgatcgc catcatcagc
    20581 aaatcgggcg gtgacttggt cgaatttacc ggtgatgccc tgctgatcat gtttcccaaa
    20641 cgagaccgaa acgatgacac cctgcacgcc gtccgtgccg ggctgcggat gcaacgggcc
    20701 atgaaagagt ttgcccatat tgaaacctcg gctggcattg tcactttgaa aatgcgcatc
    20761 ggcatccact cgggccgctt tctcacctct gatattggca ccccccgccg catggagcat
    20821 gtattgcttg ggcgggatgt acaaagagcc aaactggccg aaagcaaggg acgtaatgaa
    20881 cgcgtaaacc tgagtccttc ggcatacgaa cgagtccttg aacattttcg ctttgagcag
    20941 ggcgaaaaga tcgaaaatga aaaagaccag tactacatgc tggtggaaga tgacctggcc
    21001 gataatgaac tcggcgaata tgagatcacc ccggtcagcc gccgcatggc ttccagcaca
    21061 ctgatagacc gcgacaaaca atcggtctta aaagaaatca ccgatctggt caattccatt
    21121 gaacccatgg ccagttttct gccttttccc gtcctttctt tgctggtgga aagcgccgct
    21181 caaaggaaga tcgaccctga tttccccaaa ccgacgatca tgttcatcaa cttcgtgggg
    21241 ttgcctgagg ccgccgaccg cgccctgccc ggtgaagaac tcaagctctc gaccagcttt
    21301 tcgaaggctt tttccttaat caacgccgta gtggaatctc gtggcggcat gttgaaaaag
    21361 gtgacatatc acctttcagg ctcagacatc gtcatttatt tcggtgttcc aaccgcccac
    21421 accaacgacg agatgcgtgc cgcgtctgcc gcagtagcca tccgcgatat cgtgatgaac
    21481 atcaagccgc ccaccatcgg gaatttccaa cctgagatct attgcatgat cggaatccac
    21541 accgggcctg cctttgtcgg cgagatcggt gacccgcgcg ggcggcgcga atacaatgtg
    21601 ctgggagata ccgtcaacac caccgcccgt ttgatgaatc gcgccgggcg aaaccagatc
    21661 atcatctcag aaagcgtaca gcaggccatt gagacgaaat atgaatgcca gccgctcggt
    21721 gaagcctcac ttaaaggtaa aagcaccccc atggcgctgt acgaactggt gcagaagacc
    21781 ccaaaacctt agccgtcaac agaacactac agaactgatt ttagtctgct taatctttgc
    21841 gaatgtaata taatcaaaaa agaaataatt cataaatcct tacttggaga gggaagatgt
    21901 ccacaatcat ctcagtacat tcattccgtg gtgggacggg aaaatctaac accactgcaa
    21961 atatttcagc ccttttggcc gtggatggtg cgcgtgtcgg tgtggtggat acagatattc
    22021 aatcgcccgg catccatgtc ctttttaatc tcgatgaatc ggaaatgggc cattccctga
    22081 acgattacct ctggggaaaa tgctcaattg aagaaaccgc tcacgatgtc acttcccatt
    22141 tgggtgctga agtcaaagga caggtcttcc tcatcccttc cagcatcaaa ccaggtgaaa
    22201 tcgcccgcat cctccgcgaa ggttacgacg taggcctgct taacgacggg ttccgcgatg
    22261 tcgttgaaaa attgaaactc gattacctgc tcattgacac ccaccctggg ttaaacgaag
    22321 aaaccctgct ttcgatcgcc atctccaatg cactggtcat catcctgcgc cctgactcac
    22381 aggactatca gggaacagcc gtcactgtgg atgtggcccg caaactcgat gtccccaaga
    22441 tgctgatgct cgtcaacaag gtgccgcctc aattcgacct cgaagatgtc cgcacccgtg
    22501 tagagcagac ctacgatgcc acagtcgccg ccgtcctgcc tcattccgat gaaatgatga
    22561 cactggcaag ctctggtatt ttcgccattc gcttccccga ccacccaatg acgaaaggcc
    22621 tgcgcaaact ggtggatcag ctcaaagcct aggctatctt tccaaatgtg ccatgaacga
    22681 aacccctttt gaactagcgg tcaaggaact tgaagcaggt acggaggata tccgtccttc
    22741 cgacatcgtc tcatattcag aaccattacg ctccgctttg aatttcgtca tccgccttgg
    22801 gcggttcagc atgaccgaat ttcaggaaaa actggaattc acccgcgaag aaacaaaaaa
    22861 gatttccgat atcctcgtgg cgcgaaacct gttcgcaatt tcaaggttta caacagccga
    22921 agagacctac ttcgaatccc gcctcaccgc catgacgcgc cccctcaccc gtccgccttc
    22981 agatatctgg aagaaaatag agtaaaccaa tcacccgcct gaaaattcag gcgggttttg
    23041 attcggctat aatgcaggca tggcaacaaa atcctcctcc cggtacgaag aactcaaaga
    23101 acaggtcaac ttccacaatt atcggtacca tgtgttggat gcaccggtga tcagtgatct
    23161 ggaatacgac cgcctgctga atgaactcaa acaactcgaa gccgaccacc cagagtggat
    23221 cacacccgac agccctacgc aacgcgccgg tgcccggcct gctgaccgtt tcgagaaaat
    23281 tcgccacccc gcacccattc tcagcctggc caacgccttc ggcgcagacg atgcccgcgc
    23341 atggttcgag cggatcaaaa agcttgatga ccgcgtggaa aaagccaaat tcgtcgttga
    23401 gccaaaaata gacggcttat ccgtcgtcct gcattatcgc gatggaatgt tcatgcaggg
    23461 tgccacccgc ggcgacggtg aagtgggtga agacatcacc tcgaacctgc gcactgtgcg
    23521 ggcaattccg ctgaaaatcc ctgtgggaag cgaacagggc aggcagtcat cgcttcatcc
    23581 ctttcaagtc ccaaagcatc ttgttgttcg cggcgaagct ttcatcccca acaaggattt
    23641 tgaagcatta aataaaaaac tcgaggaagc aggcgaaaag acctacctca acccgcgcaa
    23701 tacagcggcg ggttcattgc gccagcttga tccacaactc accgcgtcac gcccgatcac
    23761 cttgctggtg taccagatcg tccattctga agggggcaag gtacccacct cccaatggga
    23821 aatcttggaa tacctcaaag ccctcggctt tcctgttacc gatgtagccc gtcgattcaa
    23881 cgacctcgaa gcggcgatcg cctacacaga aaccttcagc acaggacgcg atgcgcttcc
    23941 ctacgaagcc gacggtatgg tcataaaaat tgacgatttg accctcgccg cagatctggg
    24001 cttcgttggc aaagacccgc gcggcgcggt cgcgttcaaa ttccccgccc gtgaagtgac
    24061 caccacattg aatgatatcg gcgtggcggt aggccgcacc ggggtgttga ccccttatgc
    24121 catgctcgaa cccgttgaga tcggcggtgt ggtggtggaa agagccaccc tgcacaactt
    24181 cgattacatc gctgaaaagg atatccgcgc aggagatcgg gtgctggtga aacgcgcagg
    24241 cgaggtgatt ccctatgtca tcggccccat tgtggatgca cgcagcggca aagagaagaa
    24301 atacaaaccg ccctcaaaat gccccgcctg cggacaggaa gtggagcatc tcgaaggcga
    24361 agtggcttgg tactgtgtca atgcggcctg cccggcccag cttgtgcgca atgtggagca
    24421 tttcgtctct cgcggcgcaa tggacattgt agggctgggc atcaagatcg tagagcaatt
    24481 gatcgaagcc gggttggtca aagatgtggc tgacctgttc tcgctcaaga aggaacaatt
    24541 gctggaactg gaaggttttg cagagaaaaa ggcggagaat ctgcttgggg ctattgagca
    24601 ggccaaaggt cagagtttga atcgcttgat cgtggcgctg ggcattcatg gcgtgggtga
    24661 agtcatggcg ggagatttgg cgcgcacgtt tggaaatcta tccacattat caaaggcgtc
    24721 tgccgaggaa cttcaacaaa tggatgggtt ggggcccaat atcgccgaat cgattgtgga
    24781 ttggtttgcg caaaatgcca atcagaagtt gttgaaaaag ctgaaaacgg caggggtgtg
    24841 gccggaaatg aagaaagatg aaaagaagaa agaaggggcg tttacaggcc agactttcgt
    24901 agtgacgggt accctgccca cgctttcacg cgatggtgtg aaggaattta ttgaaagcaa
    24961 cggcggcaag gtgaccgata gcgtaagcaa aaagaccagc taccttgtgc tcggcgaagc
    25021 cccaggctca aaattggata aggcaaaatc gctgggcgtg aaagtgattg atgaagctga
    25081 gctgcggaaa ttggcagagg ggtaaagagt ggaaagtgga agttaccttt tttcatcctt
    25141 tatccctcat ccttcatcct ttattgcacc agccttcttc acattttgct agacagaacc
    25201 tttccactcg gctacacttg cgacattctt tttcggaggc actacatgga tttcaacaac
    25261 acgaacgacg ctgtcacccg ccgcatcaag gccatgacgg atcgcctgga ttatctcaag
    25321 agcaatgatc tttactttta taaccaaccc gtggaagaga ttctgccggg caacaaggtg
    25381 cgcgtacgcg ggcgtgaaat ggggatgtac gcttcttaca gttatttggg gctggtcggc
    25441 cacccacgca tcaacgaagc tgccaaaaag gccgtggaaa aattcggtac gggcacaaac
    25501 ggcgtgcgga ctctcgccgg cacactgacc atccattccg aattggaaga gaccatttcc
    25561 aatttcaagc acaccgaagc agccatcacc tacacttcgg gttatgccac caacctgacc
    25621 gtcatttcca cgctgatggg ccgcggtgac tacgtttttt cagacaagat caaccacgcc
    25681 tccattgtgg atggttgtct catgtctggc gcggaatttc gccgcttccg ccacaatgac
    25741 atggaacacc tcgaaggcct gctcaaaaat gcccccgccg atgtagccaa actggtcatc
    25801 gcggattcgg tcttcagcat ggatggcgac atcattgacc tgcccaccat ggtcacgctt
    25861 tgcaagaaat acaatgcctg gctgatgatc gacgaggccc attcggtggg cgtgctcggt
    25921 gaaaaaggca cgggcatcga agaatatttc aacatgtacg gcgaaattga catcaagatg
    25981 ggcaccctca gcaaaaccat tccgtcggtt ggcgggtatg tggctgcgag gaaggaaatc
    26041 attcaatacc tgcgccatgc cagccgcgcc tacatcttct ctgcggcgct ccctcctgcg
    26101 caggccgccg ccgccaacga agctttcaag gtcattttgg acgagccgtg gcgcatcgaa
    26161 cgcttgaacc agaacaccaa acagtttatc ggcgggctga aaagcatggg cttcgacacc
    26221 ctgctgaccg agaccgccat tgtgcccgtc ttgtgcggcg aagatgacat ggctttcgcc
    26281 atgactcgcg aatgtcagca caacgatgtc tttgtgctgc ccgtcgtttc ccctgccgtg
    26341 caggaaggtc tggcccgcct gcgtgccacc gtcactgcgg cgcatgaacc aagcgagatc
    26401 gaacgcgcaa tggatgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg
    26521 ggcaggtttc aggaagggcg aagcgttttt tcatcctgat agattttgag agaatattta
    26581 taacattttt ctaataaatt ccttgtacaa tgcgctcaca ttatcaagga gcgaaccatg
    26641 aaatggcaat ggaaattagg aaaatttgca ggtattgacg tttacgttca cgccaccttc
    26701 ctgcttttga tcgggtgggt cggctacagt cattggctcg aacatcgcaa ttggggcgag
    26761 gtactcaacg gtattttgtt catcctggcg ctgttcgtgt gtgtgatttt gcatgaatat
    26821 gggcatgccc tcacagcccg caagtacgga atcaagactc gcgatatcac cctgtacccc
    26881 atcggtggtg tggcgcgttt ggagcgcatg cccgagaagc cgatcgagga attgtgggtg
    26941 gcgctgatgg gacccgccgt caatgtggtg atcgcggctg gattgtttgc gtatctttat
    27001 ttgacctcaa gcctggtgcc gctcaatgag ctgaccatag cctcgggcaa tttcctcgaa
    27061 aggctgatga cggtcaatct ctcgttggtg ctgttcaacc tcatccccgc cttccccatg
    27121 gacggtggac gcgtgctgcg ggcactgctc gccatgcgca tggattatgt ccgcgcgaca
    27181 cagatcgccg cgaccatcgg tcagggcatg gcgttgttgt ttggcctggt gggtttgttt
    27241 ggcaatgcca cacttttgtt catcgcattc ttcatctgga tcggcgcttc gcaggaatca
    27301 ggcgccacgc aaatgaagaa cgccatcagc ggaatccctg ttggccgcgc catgctgacc
    27361 gaatacaaaa gcctttcgcc gcgtgatacc cttgcgcgca tgtcacagtt gctgctggct
    27421 ggctcacagc atgatttccc agtcatcgat ggcgagcgca ttgttggggt tgtgacccgt
    27481 gatgatttcc tcatggcact cacccagcac ggagaaggta tcgccatttc agccgtaatg
    27541 aaacgcgacc tccccgaagt ggattcctac gaaatggtgg agaacgccct gttgcgcatt
    27601 caggaatcgg gcgtgcccgc cctgcccgtt acacacggcg ggcaattggt gggcattgtc
    27661 accgctgaga atatcgccga gtatttgatg atccgctctg ctttgcgggc ttcacgcatg
    27721 gcggttccgc tgtgattgca attgcaagcc tagataagag gattcttatc cacaaaacgc
    27781 acatcttctg tatactctaa tttactccga tggggagtag ccgcccgaag tttgggtagc
    27841 acagccgtca atacggaaat gatttcccgg ttgttctgta aaaggcaaga ccatcgtcaa
    27901 atcttttggc gatggtcttt ttattttcaa aagacctgcc agatctaaaa caacagaggt
    27961 accatgtttg accaaatcga aacaacgatc ctttccgcat tacaaaccat cttcgaccag
    28021 tttggctggg cgggtgtatt cggattaatg gtattcgaga atgccacggg tattacgccg
    28081 agcgagatca tcctcgcctt cgcggggtgg atgctcatcg agcggcacgg tattgcaccc
    28141 acctttgtgc tgatcgcagg cgtgtatgcc gggttgggca gcaccctcgg cgcatccatc
    28201 acctattgga tcgcgcggct gggcggacgg cccgtcattg accgtttcgc caggttcttc
    28261 cgcattgacc ccgccctcat caacaaagcc gaagaacaaa tgcaaaaatg gggttccgga
    28321 ttggtattga tcggcagaat catccccgga attcgcacgc tcatcagcat cccggccggg
    28381 ttggcgaaaa tcccatttct gaaattcttc gcagcaactt ttatcggtgc ctatatttgg
    28441 tgtacacttc tcatcggcgc gggttatgtc cttggccacg aatggatgct ggtcagtgaa
    28501 gccattaaac agcacctgcc gctggtactc acgctgggac tgctggccat ggccgcagtc
    28561 gtcgtctatc aataccgcgc ccgcatcccc gcactggcat ggatgaacac aaagagaatg
    28621 gataacgaat catgaattgg cacttgcttg gcattcagga aagtctctca aatttaaact
    28681 cagaccaaca gaacggcctg accgaatcag aatcatcggc acgccgaaaa caatacggac
    28741 cgaacgaact gatcgaacgc ggcgggcgca ccccctggca aattttgtgg gcacaactca
    28801 ccgccaccat ggtgctcatc ctcattgctg cagcagtgct ggcgggcatc ctcggcgatt
    28861 acaaaaacac catctccatc cttgccattg tggcactgta tgccatcctc ggttttgtgc
    28921 aggaatatcg cgctgaacag gccatcgcag cgctcaaaaa aatgtcggtg ccgaatgtac
    28981 gcgtgttgcg cgacggctcg ctgaaagagg ttgccgccaa tcaactcgta cccggtgaca
    29041 tccttcaact cgaaacaggc aatgtcgtcc ctgcggatgt gcgcctgctc gaagccgtaa
    29101 acctgcggat tcaggaagcc gcgctcactg gcgaatcaga agccatcgaa aaacacacca
    29161 ccgccctttc aaacgacccc accctcacac ttccgctggg tgaccgccgc aacatggggt
    29221 acatgggcac catcgttaca caggggcgcg gtcttgcact ggtcattgcc acaggcatgc
    29281 agaccgaact cggcaaaatt gccgacctca ttcaaaaagt ggaacaggaa aacactccgc
    29341 tgcagcgccg cctcgatgcg cttggcaaaa atctcgcgct catcggcacg ggtatcgcca
    29401 tcctcatctt gatcctcggc ctgctgcgcg gcgatgaact ccgtcacatg ctgctcaccg
    29461 ccgtcagtgt ggcagtcgcc atcgtccccg aaggcctgcc cgccgtggtc accatcaccc
    29521 ttgcgcttgg cgcacagcgc atgttgcagc gcaacgcact cattcgcaaa cttcccgccg
    29581 tggaaacgct cggctcggtc acggtcattt gttccg
//
LOCUS       DAOQLC010000047        29197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_507_length_29197_cov_23.705888, whole genome shotgun sequence.
ACCESSION   DAOQLC010000047 DAOQLC010000000
VERSION     DAOQLC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 29197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29197
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_507_length_29197_cov_23.705888"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PL000_10080"
     CDS             complement(<1..650)
                     /locus_tag="PL000_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC89292.1"
                     /translation="MNQMIQLGSVNGYLAKPGGTGPFPAMIVIQEWWGLDAQTKSIAD
                     RFAQEGYLTFAPDLYHGELAQLGDGDTAMKLVQKYGPVAHNDLTALYDALKSHPDCNG
                     KIGSVGFCFGGRMSLSLSTLRPVNAVCTFYGGGMQMIFDQLRANLKAPVLGFFGDADV
                     SIPAGTIEEFDKLLDEVGVEHEVITYPNSGHAFFRDSDPSVYIPEASKDAWERAKK"
     gene            810..1283
                     /locus_tag="PL000_10085"
     CDS             810..1283
                     /locus_tag="PL000_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89293.1"
                     /translation="MPVPTRVPAKSGVIAAKTSSGSIVKFSDQLTKSVSDVAQIIEQH
                     KGMIDSIQEIALELTTSIGTLHTLTVKYARTANGILDILLPILRNLPIIPKNVMQMLV
                     TLEATTQKIIDNEVSTSKTITDVQSGLRTGDVNKIKGHAGQLQNVTKSLTAMLPK"
     gene            1518..2171
                     /gene="rplU"
                     /locus_tag="PL000_10090"
     CDS             1518..2171
                     /gene="rplU"
                     /locus_tag="PL000_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC89294.1"
                     /translation="MRIAIVESGGKQYRAVEGTTIDVDRLAYEVGHKFDFERVLLMAD
                     GDVTMVGTPTVGDIKVSATVVDHVKGPKVISFKYRPKKRIRVKGGHRHFYTRLMIDFI
                     GKAGEDRKPVAAKAVESEKKAAGGADDLTKIEGVGPKVAKVLNEAGISSFADLAGAKA
                     ADVQKALDAAGLQMMDPKGWISQAKLAAKGDWEGFEKLQGELKGGRKAKSADKKKEK"
     gene            2199..2459
                     /gene="rpmA"
                     /locus_tag="PL000_10095"
     CDS             2199..2459
                     /gene="rpmA"
                     /locus_tag="PL000_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC89295.1"
                     /translation="MAHKVGGGSSRNGRDSNAQRLGVKRYAGQFVLSGNILVRQRGTK
                     IKPGLNVLVGKDDTLFAVADGIVMFDVFHGRKRVNIIPVEAE"
     gene            2461..2997
                     /gene="rpmE"
                     /locus_tag="PL000_10100"
     CDS             2461..2997
                     /gene="rpmE"
                     /locus_tag="PL000_10100"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNC89296.1"
                     /translation="MKADIHPTAYEAKVTCASCGKTWVTLSTKKELRIDVCSNCHPFF
                     TGESARLIDIEGQVDRFYKKLSARQTYVEQQKAKEETANSPERSVDDLELTPRATDAL
                     KKVGILNIGQLLAKLGEGNAAVLEISGFGQSSLTAAKKKLRALGFEVPEVPDAPKPPK
                     APKTAEASEPSAEAGAAE"
     gene            3132..3701
                     /locus_tag="PL000_10105"
     CDS             3132..3701
                     /locus_tag="PL000_10105"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNC89297.1"
                     /translation="MKHTHGSIEVICGSMFSGKTDELIRRLVRATIAKQKVQVFKPAI
                     DVRYAVEKVASHAGSTFDAIPVEKAAEIRLKLDKDTTVVGIDEAQFFDPEVVEIAREL
                     AARDVRVLVAGLDTDFRGEPFGSMPVLMSEAEDVAKLHAICMVCGENASRTQRLVNGK
                     PARYDDPVVIVGASELYEARCRRHHEVPR"
     gene            3735..4292
                     /gene="hpt"
                     /locus_tag="PL000_10110"
     CDS             3735..4292
                     /gene="hpt"
                     /locus_tag="PL000_10110"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNC89298.1"
                     /translation="MQNYKDVLAEVLIDAETLQARVRELGTQISADYKNDDLLLICIL
                     RGGVPFTVDLSRCITTPHMVDFMAVSSYGVGKRESSGSARVTLDLQTDIRSKNVLLVE
                     DIVDSGHTISSVLQMLETRQPKSLKVCALLDKPERREAHVPIHYLGFSIPNKFVFGYG
                     LDLDEYYRNLTFVGVVDLEKYHPNE"
     gene            4315..6012
                     /locus_tag="PL000_10115"
     CDS             4315..6012
                     /locus_tag="PL000_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC89299.1"
                     /translation="MDNESSDSVQRPSPYAGRWVARVHGRVVAQGGTPEQALRASQES
                     RHKEKPEIVFMSLPLALPPLLEKLLTILPADQEIYLVGGAVRDLLLARVSPDLDFVLP
                     SNGIALARRVAKTLGADFLPLDEERDTGRVIVTNEDGTRTFLDFSIYRSAESTSVFGH
                     SLEEDLRARDFTMNAIAYNLHTGTITDPLNGGTDLRAKLIRACSPTSLSDDPVRILRA
                     IRQAALFGFHIDKQTREWMKESVGQLERISAERLRDEVFKILQGPRPDTAIRALEMLG
                     VFPHLMPELTALKGVQQSAPHVHDVWTHTLSVLGALDQIIASLRVGYDAEQTNDMFTG
                     LLTLRIGRYREQMAKHFSTSLNIDRSQRALLFFAALYHDVCKPQTKTVDENGRIRFFD
                     HEVQGADVVANRAHAFNLSNDEVDRLQAIVKHHMRIHAFASRLEHEKQTPSRKAIYRF
                     FRDAGEAGIDLILLGLADLRGTRAHELTLETWTAYLDVARLLLENYWERPEEVISPPR
                     LVGGHDLIHELNLQPGPLVGQLLELIRENQAAGRIETREQALAFAREELEKGMTDSP"
     gene            6009..6545
                     /locus_tag="PL000_10120"
     CDS             6009..6545
                     /locus_tag="PL000_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC89300.1"
                     /translation="MKLVYFDLESQNLFEDVGGRDNIAKLRVACGVTWSTQTNDFSVY
                     WEKDVPALIDELRSATKVIGFNLKGFDYLVLQPYAPQIRFASIPTVDMLLDLQKTLGF
                     RIGLDSVASATLNAAKTADGVQAVEWFRAGELDKVAEYCKADVDITRRVFEFGRDNGH
                     VFYKSKLGSKLKVAVQWK"
     gene            6553..7326
                     /locus_tag="PL000_10125"
     CDS             6553..7326
                     /locus_tag="PL000_10125"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC89301.1"
                     /translation="MKNISVNGIKLAYDRRGKGAPLVLLHGFPLDHTSWNEVASILED
                     HFDVILPDLRGFGESTTVNSPYSISDMADDIAGLLDSLGIEQVAIAGHSMGGYVALSF
                     LKNYPKRVSGLGLISSQAAADTPERKDGRYKTAVEVAEKGVSVVADAMSPKLSADERV
                     RVFVRESILRQSQAALIGALKAMATREDMSSLLASIEGQFSVVLLHGNDDVLVPIERS
                     KEIADLLKSSRLFEIPGAGHMPMMEFAKETALALDFLRP"
     gene            complement(7411..7839)
                     /locus_tag="PL000_10130"
     CDS             complement(7411..7839)
                     /locus_tag="PL000_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89302.1"
                     /translation="MTTKILVIDDDVAITELMNMLLKTHGFEVFTTNSGVEGVKLVEE
                     HRPNVVLLDLMMPDMDGWQVCKAVRSFNNVPILILSAINDPSMVASVLDAGADDFLVK
                     PVPSSVLVAHIRKMVRRNNTGTLSMGMGKSNARTTHPLPS"
     gene            8074..10917
                     /gene="ppdK"
                     /locus_tag="PL000_10135"
     CDS             8074..10917
                     /gene="ppdK"
                     /locus_tag="PL000_10135"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNC89303.1"
                     /translation="MTKWVYLFNEVKEVEKRVGGSWDDVKALVGGKGAGLLDMTRADV
                     PVPPFFTVTTEACNAYQKAGKFPAGMWDQELKALKAIESKTGKKFGDPKNPLLVSCRS
                     GAKFSMPGMMNTILNIGLTDTVTEGMIKATGNARFAWDSYRRLVEMFGTVVMDVEDEA
                     FEHPLAEFKNKKGYKLDTEMSADDWKEVVGVYKQVYKKETGFEFPQDPYKQLSLATEA
                     VFKSWNGKRAIAYRRKEGISDALGTAVNICTMVFGNMGDDSATGVAFTRNPSTGEKVM
                     MGEYLLNAQGEDVVAGIRNTDAIQNLIKYMPAAYKQFMGITAKLEKHYKDIQDVEFTI
                     ERGKLWMLQTRNGKRTAKAAVKIAVDMANEGLITKDEAVLRVTPDNVDALLHPQFDDA
                     AMNQAEKEGKYLSKGVNASPGAAVGQVYFDADMAEKMSKEEKQDTIMVRPFTKPDDVH
                     GMIASKGVLTSEGGATSHAAVVARQFGIPCVVGASAIKIDLEKRVMAVGDITVKEGDW
                     ISVDGTTGKVFVGKIPTSEPSLEEQKELMTLLKWADEIAARKDVRQSVAAGYPTRGLQ
                     VWANADYPKDARRARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSDARTAALNL
                     LLPHQRKDFDGLFEAMDGYPVIIRLIDPPLHEFMPDEEKLMEEVITMRVKGEHEGLEE
                     KEKLLAAIKGMHESNPMMGLRGVRLSISMPEIVEMQVRAIFEAAADCTLRGIKVKPEI
                     MIPLTGTVKELEWIQPRLERIGKAVMQEKKIKFAYKFGTMIEIPRAAVTADHIATQAQ
                     FFSFGTNDLTQMTFGYSRDDAERNFLITYVQEGILVKNPFQTLDRNGVGKLMQRAILE
                     GRATRPELEVGICGEHGGDPDSIEWCHIIGNNYVSCSPFRVPVARLAAAHAALKHMPA
                     KKAAVRKPAAKKAVAKKVVAKKVVKKAVKKGKK"
     gene            complement(10961..12193)
                     /gene="hemW"
                     /locus_tag="PL000_10140"
     CDS             complement(10961..12193)
                     /gene="hemW"
                     /locus_tag="PL000_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNC89304.1"
                     /translation="MTTDTSIYIHIPFCKHRCAYCDFNTYAGQEDSIPAYVDALIREI
                     GFIGDQLVKLPTFNVPTVFFGGGTPSLLSAPQFDSILKALTSAFTFTADAEVSIEANP
                     GTISPAKLDAIRKAGINRISFGVQSTNTEELRMLERIHDFFTVIEAVSTARKAGFDNL
                     NLDLIYGLPEQTLTSWQTTLERIVELQPEHISAYALTLEHGTPFGRWASKGLLPIPDP
                     DLAAEMYEFTEDFLESHGYAHYEISNWAKDRDESFSHPSSFIPHPFFSCRHNLQYWRS
                     LPYLAFGAGAHGYANGYRYSNVLRIKTYIERLANYQAPTSNYQFPLSPATVNHKKQSV
                     QEDISDYMINNLRLVNAGVAESDFRLRFGSGLLDVYPKEVEKLLRNDLMEHVKESDIY
                     RLTKRGRLLGNQVFMHFV"
     gene            complement(12190..13341)
                     /locus_tag="PL000_10145"
     CDS             complement(12190..13341)
                     /locus_tag="PL000_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89305.1"
                     /translation="MSFAYLLIEFLDELTYGISEAAWPFIRDDLHLNYVQIGLALSLP
                     GILGNIIEPFIFTFADAWKRRVVLLTGGIFFIISLALTGISTSFLFLLSSYILFNPSS
                     GALVGLSQASLMDLQPERREQNMARWTFAGSLGVVIGPILLSGAAYIGFGWRGMFLGL
                     ACIAILILLFVWKRIPTDQTTTEEIPGFRAILAQFKSTFAALKNWGIVRWLILLEFSD
                     LMLDVLYGFLPLYFVDVAGFSPAQAALSVVVWTGVGMLGDFLLIPLVERIKGIDYLRI
                     SVIIELILFPLFLLSSQAWLKLTIVGMMGFFNAGWYAILKANLFESMHGQSGAALVLD
                     NVSGLFGKLIPFGIGLAAQTFGIGSAMWILIAGPLALFIGLPRKHTSRA"
     gene            13698..14081
                     /gene="speD"
                     /locus_tag="PL000_10150"
     CDS             13698..14081
                     /gene="speD"
                     /locus_tag="PL000_10150"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNC89306.1"
                     /translation="MNATLKLGEHFIFDLSDCNHEILMDSERAYSLFAQAVRESGLTV
                     VDEGFYKFSPHGFTCFLLLAESHASLHAWPEHNYCAIDLFTCAIGKDMMPLLMKIKQA
                     FGADDFSMRKLDREASVETRLPIAV"
     gene            14097..15038
                     /locus_tag="PL000_10155"
     CDS             14097..15038
                     /locus_tag="PL000_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89307.1"
                     /translation="MSETTKPPLYWIADYLTPDDVLQHAIHKVILHKHTAFQEMWIVD
                     CGTYGKGLVLDGIWQSSTGDDFLYHESLVQPACVNHGNPKRGLVLGGGEGATIRELLK
                     WKAMERVVMVDLDGEVVEACKIHLPEMHQGAFDDPRVELVIQDAVAYLDSSPSEWDVI
                     ISDLTDPIESGPSFKLFTKEYFSKAQKALRPGGYFLLQAGPVSPPSMDMHIRLVSTLK
                     AVFKYVHSYHIHVPMLGHPWGFILASDSPINTRPEPAEVDQLLAEKTNGIFKMFDGIT
                     LLGLMQSPRHIRDAIAKETTVYTLEHPPVFSTQELPK"
     gene            15134..15961
                     /gene="speE"
                     /locus_tag="PL000_10160"
     CDS             15134..15961
                     /gene="speE"
                     /locus_tag="PL000_10160"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNC89308.1"
                     /translation="MSIWFTEELHPYYRKGIRIKNILADEQTPFQHVQVVETEFFGKA
                     MILDGIIQLTERDNMGYHEMIVHVPMLAVGQPKRVLIVGGGDGGSLQQVLRYPSVEEA
                     IVCELDQRVVDLSREHFASFGDPWADPRARLLVRDAFGYLEENPSQFDVIISDTTDPI
                     GMAERLFSEEFYKLMVRALTPNGAAATQCEQPFFDTELIKQIHHSAKTLTKNPAYYYA
                     NIPTYPGGGIGFMYVSNTPWENGLKTPFPPGKNQYINPEIHKAAFALPEFFRRELMG"
     gene            16100..19060
                     /locus_tag="PL000_10165"
     CDS             16100..19060
                     /locus_tag="PL000_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC89309.1"
                     /translation="MSKLIVSYSRKDSVVARKLIDTFKKDEFDVWVDWEDIPPAVGWL
                     EQILRGIEESDAFIFLISPDSVNSEVCKVEIEHAAKNNKRIIPIVLREVDPKTVIPTI
                     RDLNWIFIREQDDFAAGIEKVKVAITLDIEWVEEHRRLQVRALEWHREKDPSHLLRGH
                     DLRTARQMVASAESKDPKPTELQRTYIKYSINDETRRTTLFISAATALLVMIILSITA
                     FYQSQKASANEKLAQEQKLLAQNNEQLAKKNAETAEKNAAIAVKNQEVAEQNQLIAEA
                     QRSAARAQIYQSRTGGLYISTLLALDSMRRSPSQEAEDILRKNISLLPVPVAQMEQDD
                     IINALEISSNDDAFVTASADGTVCVWNLPDGKMRFCATSPEAVEDAAFSPDGKLIATG
                     DTSGVVMILDAQSGEIQNTYEYGVPIWDVNISPDGKSLAAARDDGRITFVSLVNRKFS
                     FELITFGGLYVTAFSPDGTWIAAGSNEGTITFWNLGNGKVLNGPSHHGEVFEIAFSPD
                     SNRLISGGSDSVAYMTLVSTGEELFKIINEDWVEDLTFSPDGKWFVTVSDDQRIRVWD
                     TATGKEKLRMLQDSFLSSVEVSPDGQWLATTGYDKTVRVWNAATGAEMFQIPINANGN
                     MLAFSRDGKYLISGDQAGEINVWDISALPASSSYLQMGALASNVQYSPSGEWMVASDG
                     NEVWLLTKEQLAAQSGLLKGSPTFEIDSLIQDLIISPDSKWVAVTTDDAEVILYNVAS
                     KSKKLVTEFNDVPSIVFSADGSQLITGDYDGSVQAWDTASGALVADLFDTDQNVNFLA
                     INAGQLAIGMQDQIVILDAATGEAISTIESPGDHQLLAFSADGSLLAANNTSGQVYIW
                     QKQAGGFDLLQNIPSEQAASMIFNPKGDRLLIGALNNVFVLDPFTGGEITRIRHKDTV
                     NSMSYSVDGVTLATSSFKAIQFWDMQKMSQLQGDDLIKAACNRLTQNFDTAQWTTFFG
                     EEPYQVLCENLPVP"
     gene            19230..21224
                     /locus_tag="PL000_10170"
     CDS             19230..21224
                     /locus_tag="PL000_10170"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC89310.1"
                     /translation="MIALLWRQNHRSLNGLGFWLADFVLQFTGLVLVSLREILPDFFP
                     VIVANTMVIGGTILLYIGLVRFLDTRRPQIHNYIFLALFVSLQVYFLEIHPSLEARNI
                     NLSAGLFFICIQSAWLMLRGKKIVSPPERGVGLIFLAYCFVSMVRIMTYVVDSPGENF
                     FAEATAFDAISILTYQMLFIALTFSLVLMVNQKLADEAAASMDMLHNSREILRFSEEK
                     FSKAFHASPDAILISRASDGKIIEVNEGFSRMLGYSHEEALTKHNLILQLWADPKDWE
                     NVLGLIRENDHVHNCEYGFVTRSDQKLYCLYSGEYINLGGETCILSIVRDITERRLVE
                     AELRESEMKYRLLFETMAQGVVYQDKDGQITFANPAAEKILGLTLAQMQGRASIDSRW
                     NAIHEDGTPFPGETHPAMVALRDGVAVFNVVMGVYNPQTDERHWININATPEFKPGEL
                     TPYQVYATFEDITQRMLVEEELRKTNELLHSQLDEIQSLHDALREQALRDPLTGLHNR
                     RYLNETMGREIARAKREKYPISIMIVDVDNFKQFNDTYGHSLGDKVLVAFGKVLHENT
                     RVGDIACRYGGDEFLVVMPGADIMGASQRAEDIRQKFAATPVYYGDSEIFATISIGLS
                     TYPQHGIDSSLFRSADEALYHAKHSGRNRIFIWSEKKMFG"
     assembly_gap    21227..21326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21327..21800
                     /locus_tag="PL000_10175"
     CDS             <21327..21800
                     /locus_tag="PL000_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC89311.1"
                     /translation="NNISSLWSDCMLVRDLMTIYPVSISIHSDLRRAAEIVSLAGASD
                     LMVVDTERRFLGVLSEGDLIRAIMPNFDEVLEAGGTLDDAFNFFIRKGKDLASRPIEP
                     LVIKNPIVVSPTDEAAQVATILIQKQIRRLPVVDNQLLIGTVSRADICRAVIYSA"
     gene            21821..25309
                     /locus_tag="PL000_10180"
     CDS             21821..25309
                     /locus_tag="PL000_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HNC89312.1"
                     /translation="MLPSRLAFENAVSFNQKHGHENLGFVSAQWGFLPVKPPLLALPD
                     YFKAWDVIAADLPSLFRTLQVRRAVDALPYLNVDEHHLPAEYLLRASSLVSILLHAYV
                     RVEASAPATLPSHLMQAWEGISQRLDRPAPILSYVDLILYNWKLRDPQASPLMLVENL
                     DLLFPTVGNEEERVFYLTQLEIAAACAPLMLAVIRAQEAASRDDVPALERELHLMLDV
                     FRHASEFSVHKIDPNPYSRTYVDQVVWAKTVAPFAVPFIKGRVGPSGTAAPIFMYMDA
                     FFGRESHASTLGRETENLLTYSPLHWRTLVEAVGKFSVRNYIARSGNRALQGLFDTLL
                     EAYAGDKGYLGTHRLKAYGFLEIAFKAGRSLTIGGFKGLFRDKTWNQIDDELGHARNE
                     RYLGLTPHLYSLHLENGESSFPVSRNWITSLTLETRSDEMHYRPGDRISLLPENSPEL
                     IDRTLKALHAVGTESIHLNHMWQAHLQFRSGYSHETNSLPLSDLLRFGQIRPVNRATA
                     KALFKITGSSFLGRILNARMEDQWELWDLLQMLERGGFDPRQLWKAEPWEPEHITRLV
                     RPELPRLYSIASSPSLKKKLEISVAGLEYDSVESPVSNQFPRQGVASSFLRRLAEQAE
                     GQTELTVRLSPSARFHLPADADRPIVMFAGGSGIAPFAGFLQERMQSDLSRNWLYFGT
                     RTEAEIHHRDLLEKLAGAGRLHLRVALSREDKQIFLQDGRLAARSGTACHVDSLLDQD
                     AETLWSLLRNSDEGGEQACFYICGRTGFAGSIISALRNVIRRFVDSDSKANDFFRQLM
                     ADRRIMLDIFTTYGGTSDQAGRVFDISDLVLHNNDSNGYWMALNGKVYDLSEFVHLHA
                     GGRRILANNAGIDATRAYQSVQHHLNSDVDAQTGMYEIGRMRRLDLGMRWGVGLGLQG
                     LVFFTLEEAFTFWVRYLYLVTEMENALINEFSFLDRSTTLGEDPREFTPLKAHLVAEA
                     QKRFLGTFLDGILGEDLNVLWAITAGLCDRRSHYNWMPTSIQDTTLSASALHARANYL
                     QTWIDEWRSQDEEVHKTRFEQAVTQLSSIIRAENLRCFHEIKLAVRNGVQAFERHEAL
                     VIEKDGAGLISTLQQIPVIVSSYYERLGGEMQKLAQEYGVPLKSVVNSAQVDFQDLGH
                     GAHMDVTANPRIDTDG"
     gene            complement(25370..26758)
                     /locus_tag="PL000_10185"
     CDS             complement(25370..26758)
                     /locus_tag="PL000_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNC89313.1"
                     /translation="MNNQLKNDLDNLSSLLEQTRQASENYLRELAIRPPAAAYSKKEN
                     LKISVQGIGAEKTLQMFLERFGDHMPASNGPRFWGLVTGGTTPASLLGDWLTSVYDLN
                     LSHAGNSVAPDIEVEAISMLRELFGLPASFSGTFVTGATMSNFAGLAIAREWIGRQHG
                     KSVSQEGLQAIPPVRILSCEAHASSFKALGMLGLGRNSLKVIPRLPENREAVDVIELR
                     KALEELNGEPCVVIANAGTVNTVDFDDIEAIAAMKSKFKFWLHVDAAFGGFAACSPKF
                     EHLLKGIEHADSITIDAHKWLNVPYDSAMIFTQHRDLQIAVFQNSGAYLGAIAEPVDF
                     IHLSPENSRRMRALPAWFSLMAYGREGYRDIVERNCEMAEQLGMKISASKEFRLLAPV
                     RMNVVCFTFHDAVDTERVKTFLANLRDDGRVFMTPTTYMGTPAIRAAFSNWRTTETDL
                     EIAWQAMTKHAA"
     gene            26908..27540
                     /locus_tag="PL000_10190"
     CDS             26908..27540
                     /locus_tag="PL000_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC89314.1"
                     /translation="MIPHENSPVYAAGAKLENASAAMILLHGRGASAQDILSLASHLD
                     FPGIAYLAPQAEGFIWYPNRLIAPVESNEPYLSAAIAKIDRLVKQVEASGIPAEKIFI
                     GGFSQGASLSSEFVIRNPRPYGGLLVFSGGYIWQSGKPRETSGDLGGMPVFIGCSNVD
                     PFIPLERVKETSALLASMNANVTEQIYPNMDHTIIDEEIELARQLLERRL"
     gene            27708..28904
                     /locus_tag="PL000_10195"
     CDS             27708..28904
                     /locus_tag="PL000_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNC89315.1"
                     /translation="MPEFAKRMAYMQSTADVIRYLFESMTDPDVISFGGGAPAGEALP
                     VELVHEIAKEVLVREKRGVQALQYGNPIGIPDLRQAVIDHLLLPKGLRVGMENVMIVA
                     GGMETMNLVSQLYLEPGDIVLVESPTFLQSIQIFKMFEARCIACETDDQGLVIDDVET
                     KIKQYHPKMIYVVPTFQNPSGRTTSLERRKALARLGSRYDIVVLEDDPYCEMRYSGEV
                     LPPIKYFDETGNTIFANSLSKIFAPGTRLGYIFADAPIIRKFYDAKTATNSHTNVVTQ
                     ILCAEFFNRGLFAPHLQAIRNIHRERRDVMMDCIKSMLPAEVRSIYPDGGLFTWVELP
                     EHVDTTSMLEEAKQHKVAYMPGREFFVDGQPIRNNCMRVSFGGVPPEKIRLGMERLAE
                     VICSKL"
     gene            complement(28953..>29197)
                     /locus_tag="PL000_10200"
     CDS             complement(28953..>29197)
                     /locus_tag="PL000_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNC89316.1"
                     /translation="SNLGMYDVEEFIAIVNPPEAAILAIGSAKETPVVVNGEIKIGSR
                     MKATISVDHRVSDGAEAAQFMQKMAEFLENPVRMLV"
BASE COUNT         7164 a         6938 c         7560 g         7435 t
ORIGIN      
        1 aattttttcg cacgttccca ggcatctttg gaggcttctg gaatgtatac actgggatcg
       61 ctatcacgga agaaggcatg accggagttg gggtaggtga tgacctcatg ctccacgcca
      121 acctcgtcaa gaagtttgtc aaattcttcg atggtgcccg cagggatcga gacatccgca
      181 tcgccgaaaa atccaagtac gggggctttc aggttggcgc gcagttgatc gaagatcatt
      241 tgcataccgc cgccgtaaaa tgtgcagacc gcgttcacag ggcgcagggt gctcaagctc
      301 agtgacatac gtcctccaaa gcaaaagcca acactgccga tttttccatt gcaatcgggg
      361 tgcgatttca gtgcgtcgta gagcgctgtc aggtcattat gggctacagg gccgtatttt
      421 tgcacaagtt tcatcgcggt gtcgccatct ccaagctggg ccagttcacc gtgataaagg
      481 tctggggcaa aggtgaggta gccttcctgt gcaaaacgat ccgcaatgga tttggtttgg
      541 gcatccaacc cccaccattc ttgaatcacg atcatggcag ggaaggggcc cgtaccgcct
      601 ggttttgcaa gatagccatt cacacttcca agttgaatca tttggttcat tttttcctcc
      661 gagttagggt gcgtgagtcg ttttgcagga tatacgagat acagatgttt gtcaataagg
      721 ttgtttttgg aaatgaagta ttgtatagtt gggagaagtt acctttgagt tcaggtaagc
      781 gtcatccatt attttcagga ggtttcccca tgccagtacc gaccagagtc ccagccaaaa
      841 gcggtgtgat cgccgcgaag accagttcag gttcgatcgt caaattttcc gatcagttga
      901 cgaaatctgt gagcgatgta gcgcagatca ttgagcaaca caaaggaatg atcgattcga
      961 tccaggagat cgccctggag ttgaccactt ccattggtac cctgcacact ttgacggtta
     1021 aatatgcccg taccgccaat ggtattttgg atattctcct gccaattctc aggaatttgc
     1081 ccatcattcc gaagaatgtg atgcagatgc ttgtgactct tgaggctaca acccaaaaga
     1141 tcattgataa tgaagtgtcc acatccaaga cgatcactga tgtgcagtca ggtctgcgca
     1201 caggcgatgt gaacaagatc aagggacatg cggggcagct tcagaatgtg acgaagtccc
     1261 tgaccgcaat gcttccaaaa taggcggttt atcccctggt ttataaagaa aaatccgtcg
     1321 tttcccgctt ttcaactgcg aaatggcgga tttttgcaca ttggaatcgt tgacaggtgc
     1381 gcctccctgc atgtataata ccgcgcgcta aaaatttaac ccagcttccc cacggttcgg
     1441 cgtagacctc ccctgtatgt tgacgggtaa agtggtccgc gggatgcgcg gcgaagtgat
     1501 gactggagaa atatataatg agaattgcaa tcgttgaaag cggcggcaag caataccgcg
     1561 ccgttgaagg caccaccatt gatgtggatc gcctcgccta cgaagtaggc cacaagtttg
     1621 actttgaacg tgttctgctg atggcagatg gtgatgtgac aatggtcggc accccaactg
     1681 tgggtgacat caaagtctcc gctactgttg tggatcacgt caagggcccc aaggtaattt
     1741 ccttcaagta tcgccccaag aaacgcatcc gtgtaaaagg tggtcatcgc cacttctata
     1801 cccgtttgat gattgatttc attggcaagg ctggtgaaga ccgcaaaccc gttgccgcaa
     1861 aagccgttga atctgaaaag aaggctgctg gcggagcgga tgatcttacc aagatcgagg
     1921 gtgttggtcc gaaagtggcc aaggtcttga atgaagctgg catttcctca tttgccgatc
     1981 tggctggtgc caaagctgcc gatgttcaaa aggcgcttga tgctgctggt ttgcagatga
     2041 tggacccgaa gggctggatc tcccaggcaa aactcgccgc caaaggcgac tgggaaggtt
     2101 tcgaaaaact tcagggcgaa ctgaagggcg gccgcaaagc caagtccgct gataagaaaa
     2161 aagaaaagta aagctgttat tagtgaagcg aggtagaaat ggcacataaa gtaggcggcg
     2221 gatcaagccg caacggtcgc gacagcaatg cccaacgctt gggcgttaag cgttacgccg
     2281 gtcaatttgt tctctctgga aatattctcg ttcgccagcg cggcaccaag atcaagccag
     2341 gcttgaatgt gttggttggc aaggacgata ccctgtttgc cgttgcagac gggattgtta
     2401 tgttcgacgt attccatgga cgcaaacgcg tcaacattat cccggtcgag gcggaataga
     2461 atgaaagctg atatccatcc cactgcttac gaagcaaagg tcacttgtgc ttcctgtgga
     2521 aagacctggg tgacactttc caccaaaaaa gagttgcgca ttgatgtgtg ctccaattgt
     2581 catccgttct ttaccggtga atctgcgcgc ttgatcgaca ttgaaggtca ggtggatcgc
     2641 ttctacaaga agctttccgc acgccagacc tatgtcgaac agcagaaggc caaggaagaa
     2701 accgccaatt ctcccgaacg ttccgtggac gatttggaac tcactccccg tgccactgac
     2761 gccctcaaga aggtcggtat tttgaatatc ggccagttgt tggccaaact tggtgaaggc
     2821 aatgccgctg tgttggaaat ctctggcttt ggtcagtctt ccctcaccgc cgccaagaag
     2881 aagttgcgcg ccttgggctt tgaagtcccc gaggttcccg atgcgcccaa gcctcccaaa
     2941 gccccgaaga ccgctgaagc cagcgaacct tccgccgaag caggggcagc cgaataggat
     3001 ttccctttcg ggtactttgt caggtatagg tttctcaggt aaactagaca ggcagatgct
     3061 taagcatctg cctgtttttg taaaatattt ttttcaggct ggttaattta taaaaaattg
     3121 ggagaatctg aatgaagcat acgcatggtt caatcgaagt catttgcggg tcaatgttca
     3181 gcggaaagac cgatgaattg atccgccgtc tggtgcgtgc caccattgca aaacaaaaag
     3241 tgcaggtgtt caagccagcc attgacgttc gatatgccgt tgagaaggtg gcatctcatg
     3301 caggctcaac cttcgatgca attcccgttg aaaaagcagc agagattcgt ttgaagctgg
     3361 acaaggatac aacggtcgtt ggtattgacg aggctcaatt cttcgaccct gaagtggttg
     3421 aaattgcccg cgaattggcg gcacgagatg tgcgtgtact ggtggctggg ctggataccg
     3481 attttcgcgg tgagccgttt ggttccatgc ctgtattgat gtctgaagct gaagatgttg
     3541 caaaacttca tgccatctgc atggtctgtg gagaaaatgc ctcacgtaca caaagactgg
     3601 tcaatggcaa gcccgcccgt tatgatgacc ccgttgtgat cgttggcgct tccgaactgt
     3661 atgaggcacg ctgccgcagg catcatgaag ttccacgcta ggtcaaaact ctcgaacaca
     3721 accaggataa tcccatgcaa aattacaaag acgttcttgc cgaagtattg attgacgcgg
     3781 agaccttgca ggcacgtgta agagaattgg gcacgcagat cagtgctgat tacaaaaacg
     3841 atgacctcct gctcatttgc attctgcgcg ggggggtgcc ctttaccgtg gatctcagcc
     3901 gttgcatcac cactccgcac atggttgatt ttatggcagt ctcatcctat ggggtgggaa
     3961 agcgcgaatc aagcggctca gcccgcgtca cccttgacct gcaaacagat attcgttcca
     4021 agaatgtcct gctggttgaa gatattgtag atagcggcca taccatctct tcggttttgc
     4081 aaatgcttga aacccgccag ccaaaatcat tgaaagtttg cgcactgctt gataaacctg
     4141 agcgccgcga ggcccatgtt cccattcatt accttggctt ctcgatcccg aacaaatttg
     4201 tatttggtta tggccttgac ctcgatgaat actatcgcaa cctgaccttt gttggtgtag
     4261 tggatctgga aaaatatcac cccaacgaat aatccctgcc accaacggct gaaaatggac
     4321 aacgaatctt ctgactctgt gcaaagacct tcaccatatg ccggtcgctg ggttgctcgt
     4381 gtgcatggaa gggttgtggc tcaaggcggc acgccagaac aggcattgcg tgcctcacag
     4441 gaaagccgtc acaaggaaaa accagagatc gtttttatgt cacttccact tgcacttccc
     4501 ccgttattgg aaaaattgct gaccatcctg cctgccgacc aggagattta cctcgttggc
     4561 ggggcagtgc gtgacctttt actggctcgc gtctcacctg accttgattt tgttctgcca
     4621 tcgaatggca ttgctcttgc tcgaagagtt gccaaaaccc tcggcgctga ctttttgcct
     4681 ctcgacgagg agcgtgacac ggggcgtgtg atcgtcacca atgaagatgg cacccgtact
     4741 tttcttgatt tctccattta tcgcagcgca gaatccactt ccgtttttgg tcatagcctc
     4801 gaagaagact tgcgcgcccg cgatttcacc atgaacgcca tcgcctacaa tttacataca
     4861 ggtaccatca ccgacccctt gaatggcggt accgacttgc gggcaaaatt gatccgtgca
     4921 tgttcgccaa cctctttgtc tgatgacccc gtccgcattt tgcgtgcgat tcgtcaggcc
     4981 gccctttttg gattccacat tgataaacag actcgcgaat ggatgaaaga atctgtcggc
     5041 caactggagc gtatttcggc cgaacgcttg cgtgatgagg tattcaaaat tctgcaaggc
     5101 cccagaccgg atacagccat ccgcgccctg gaaatgctgg gtgtctttcc acacctcatg
     5161 cctgagctga cggccttaaa gggagttcaa caatctgcac cgcatgtgca tgatgtttgg
     5221 acacatacat tatccgtgct tggtgcgctt gaccagatca ttgcctcctt gcgagttggt
     5281 tatgacgccg aacaaaccaa cgacatgttc acaggtctgc tgactttgcg gatcgggcga
     5341 tatcgtgagc aaatggcaaa gcatttttcc acctcactta atattgaccg ttcccagcgc
     5401 gcgcttctat tctttgcagc gttgtatcat gatgtatgca agccgcaaac caaaactgtg
     5461 gatgagaatg gccgtatccg tttctttgac catgaagttc aaggtgcgga cgtcgtggca
     5521 aatcgcgctc atgcttttaa cctgagtaat gacgaagtcg atcgcttgca agccatcgtg
     5581 aagcatcata tgcgcatcca tgcatttgcc agccgattgg agcatgaaaa acaaacgccg
     5641 tcacgtaaag ccatttatcg cttcttcaga gatgcgggtg aagcgggcat agatctcatt
     5701 cttttagggc tggccgattt gcgtggcaca cgtgcccacg aacttaccct cgaaacatgg
     5761 accgcctatc ttgatgttgc ccgtctcctg cttgagaatt actgggaacg cccggaagaa
     5821 gtgatttccc cgccgcgcct tgtgggtggt cacgatttga tccatgaact gaatcttcag
     5881 cccgggccat tggtggggca acttttggaa ttgattcgtg aaaaccaggc cgctggcagg
     5941 atcgaaacgc gtgaacaggc cctagccttt gcccgcgaag aattggaaaa aggaatgaca
     6001 gattcgccat gaaactcgtt tattttgatc tggaatcaca aaaccttttt gaagatgttg
     6061 gcgggcgcga taacatcgct aaacttcgcg tggcatgcgg ggttacatgg tcaacacaaa
     6121 caaatgattt ttcagtgtat tgggaaaaag atgtccctgc attgatcgat gagctcaggt
     6181 ctgcaacaaa ggtgatcgga tttaacttaa aggggtttga ttatcttgtg ctccagccct
     6241 acgctccgca aattcgcttc gcatccattc caaccgtgga catgctcctg gatctgcaaa
     6301 aaactctcgg ctttcgcatt ggcttggatt cggttgccag tgccaccctg aatgctgcca
     6361 aaaccgccga tggcgttcaa gcggtggagt ggttccgtgc tggagagttg gataaagtag
     6421 cagaatactg caaagccgat gtggatatca cacggcgcgt ttttgaattt ggccgtgata
     6481 atggccatgt tttttacaag tcaaaactgg ggagtaagtt gaaagttgcc gtacagtgga
     6541 agtgagagag gtatgaaaaa tatttcagtc aatggaatta aattggcata cgaccgtcgt
     6601 ggaaaggggg ctccccttgt ccttcttcac ggttttcccc ttgaccacac cagttggaac
     6661 gaagttgcct ccatattgga agaccatttc gatgtcatcc tccccgatct gcgcggattt
     6721 ggtgaatcca caaccgtgaa ctccccatat tcaatctcag atatggcaga tgatattgcc
     6781 ggtctgttgg attctttggg catagagcag gttgcgatcg ctggtcattc catgggtgga
     6841 tatgtggcgc tgtctttcct gaaaaattat ccaaaacggg tgagtggttt agggctgatc
     6901 tcttcgcagg ctgcagcaga tacaccggaa agaaaagacg gtcgttacaa aaccgctgta
     6961 gaggtcgctg aaaaaggggt gagcgtggtc gccgatgcaa tgtctccaaa gttatctgcc
     7021 gatgagcggg tacgggtgtt tgtccgtgag tctattcttc ggcaaagcca ggctgcctta
     7081 attggtgcat taaaagcaat ggcaacacga gaagatatgt catctttgct tgcctccatt
     7141 gaaggacaat tctctgtggt attgctccac ggaaatgacg atgtgctggt ccccattgaa
     7201 aggtcaaaag aaatagcgga tcttttgaag tcgtcacggt tgttcgaaat ccccggtgct
     7261 ggtcacatgc caatgatgga atttgcaaaa gagactgcgt tggcgcttga ttttttgagg
     7321 ccgtgagtct ttctcccatg taaaaaccat aaagcccatc ctttgaataa aaaggatggg
     7381 ctttatttat gcaagatcca aagagttgta tcaggatgga agcggatggg tggtgcgggc
     7441 gtttgattta cccattccca tgcttaacgt tcccgtattg ttgcgcctga ccatctttcg
     7501 gatgtgggca acaaggacgc tgctcggcac tggcttaacc agaaaatcat ctgcgcctgc
     7561 atctaaaacg ctggccacca tgctgggatc attgatcgca gaaaggatga ggatggggac
     7621 attgttgaag gagcgtaccg ctttgcaaac ctgccatcca tccatgtctg gcatcattaa
     7681 gtcgagtaga acgacgttgg gacgatgttc ttcaaccagc ttcacacctt ccacaccgct
     7741 gttggtagtg aaaacttcaa atccatgagt tttcaacagc atgttcatca actcagtgat
     7801 tgcaacgtca tcatcaatta cgagtatctt agttgtcata cttttttcct tgccatatta
     7861 tccctgattt gcccccggaa tcactttgca gggtattaac agaattttgc aatgttcatt
     7921 gcattttaat aatggaaaat atgggatttt gcctcctggg tttacagatt gatatcagag
     7981 tatctggtga tttttgtcat gtgtgaatta gagtgttttt ggtaaaatat ttcacaaatg
     8041 aatttgaaaa tctattcctt ttaggaggct gaaatgacca aatgggttta tctttttaac
     8101 gaggtaaaag aagttgagaa gcgcgttggc ggatcatggg atgatgtcaa ggctcttgtc
     8161 ggtggcaaag gtgcaggtct gttggatatg actcgtgcag atgtgccggt tcctcccttc
     8221 tttaccgtaa cgacggaagc atgtaatgct tatcaaaaag ctggaaaatt tcctgcaggt
     8281 atgtgggacc aggaactcaa agctctcaag gccatagaaa gcaagactgg caagaaattt
     8341 ggtgatccga agaacccgct tttggtatcc tgccgatctg gcgcaaagtt ttccatgccg
     8401 gggatgatga acacgatcct caacatcggg ttgactgata cggttaccga aggcatgatc
     8461 aaagcgacag gcaatgcccg cttcgcatgg gactcgtacc gccgcctggt tgaaatgttc
     8521 ggcacagtgg taatggatgt ggaagacgaa gcctttgagc acccgttggc tgagttcaag
     8581 aataagaagg gctacaagct cgataccgag atgagcgcgg acgattggaa agaggtggtg
     8641 ggggtataca agcaggtcta caagaaagaa actggttttg aattcccgca ggatccctat
     8701 aaacaactat cactggcaac cgaggctgta ttcaaatcat ggaacggaaa acgtgccatt
     8761 gcttatcgcc gcaaggaagg catctcggat gcgctcggca ccgccgttaa tatttgtacg
     8821 atggtattcg gcaatatggg tgatgactct gccaccggcg tggcattcac ccgcaaccca
     8881 tccactggcg aaaaagtgat gatgggcgag tatctgctca atgcccaggg tgaagacgtg
     8941 gtggcaggca tccgcaatac ggatgcgatc cagaacctca tcaaatacat gcccgcggct
     9001 tataagcagt tcatgggcat tactgccaag ctcgaaaagc actacaagga tattcaggac
     9061 gtcgaattca ccattgaacg cggcaaattg tggatgctcc aaacccgtaa tggcaagcgc
     9121 accgcaaaag ctgctgtgaa gatcgccgtg gatatggcga acgaaggctt gatcacaaaa
     9181 gacgaagctg ttctgcgcgt cactcctgat aacgtggatg cattgctcca cccgcaattt
     9241 gatgatgcgg ccatgaatca agctgagaag gaaggtaagt acctctcaaa gggcgtgaat
     9301 gcctctcccg gtgcagcagt tggacaggtc tattttgatg cggatatggc tgagaagatg
     9361 tcgaaggaag agaaacagga caccatcatg gtgcgtccgt tcaccaaacc cgatgatgtc
     9421 cacggcatga tcgcatccaa aggtgtgttg accagtgaag gtggggcgac atctcacgct
     9481 gcagtggtgg cccgccaatt tggtatcccc tgcgtagtgg gcgcttctgc catcaagatc
     9541 gaccttgaaa aacgtgtcat ggctgttggc gatatcaccg taaaagaagg ggattggatt
     9601 tcagtggatg gaaccactgg caaggtgttt gtcggaaaga tcccaacctc tgagccttct
     9661 ctcgaagagc agaaggaact gatgaccctg ctcaaatggg ctgatgaaat cgccgcccgc
     9721 aaagatgtcc gccagtctgt tgcggctggt tacccgaccc gcggtttgca ggtttgggcc
     9781 aatgccgatt atccgaagga tgcccgccgc gcccgctcgt acggtgctgt tggtatcggc
     9841 ttgtgccgca ccgagcacat gttctttgag cctgagcgcc tgccgatcgt acagcgcatg
     9901 atcctggctg agacaagtga cgcccgtact gccgccctca acctgttgct cccgcatcaa
     9961 cgcaaggact ttgacggtct ctttgaagcg atggatggct accctgtcat catccgcctc
    10021 attgacccgc cgctgcatga gttcatgcct gatgaagaaa aactcatgga agaagtcatc
    10081 accatgcgtg tgaagggcga acatgaaggg ctggaagaaa aagaaaaact actcgcagcc
    10141 atcaaaggca tgcacgaatc caacccgatg atgggcttgc gcggtgtccg cttgagcatc
    10201 tccatgcctg agatcgtcga gatgcaggtt cgcgccatct tcgaagccgc tgccgattgc
    10261 accctgcgcg gaatcaaagt aaagcctgag atcatgatcc cgctcactgg caccgtgaaa
    10321 gaacttgagt ggatccagcc gcgcctcgaa cgcattggca aggccgtgat gcaggaaaag
    10381 aagatcaaat tcgcctataa attcggcacc atgatcgaaa ttccccgcgc cgcagtgacc
    10441 gctgaccata tcgccacaca ggcacagttc ttctccttcg gcaccaacga cctcacccaa
    10501 atgaccttcg gttattcccg tgacgatgcc gaacgtaact tcctcatcac ctatgttcag
    10561 gaaggcatcc tcgtcaagaa tccgttccaa accctcgacc gtaacggcgt aggcaaactg
    10621 atgcagcgcg ccattcttga aggccgcgcc acccgccccg aactcgaagt gggtatctgc
    10681 ggtgaacacg gcggtgaccc tgactcgatc gagtggtgtc acatcatcgg taacaactat
    10741 gtttcctgct cgcccttccg cgtgcccgtt gcccgcctgg ctgccgctca tgctgcattg
    10801 aagcacatgc ccgccaaaaa ggctgctgtc aggaagcctg ctgccaagaa agcagttgca
    10861 aagaaagttg ttgcgaaaaa agttgtgaag aaggctgtaa agaagggtaa gaagtaattt
    10921 gaaaatcccc gccatctttt tcgatggcgg ggattttttg ttacacaaaa tgcatgaaga
    10981 cctgattccc caatagcctg ccgcgtttgg tgagcctgta aatatcggac tcttttacat
    11041 gctccatcag atcgttgcga agaagtttct ccacttcctt tggatagaca tccaaaagcc
    11101 cgcttccgaa ccttaacctg aaatctgatt cagcgacacc ggcattcacc agcctgaggt
    11161 tgttgatcat ataatctgaa atatcttcct gcaccgattg tttcttgtgg ttgactgtgg
    11221 caggtgagag gggaaattgg taattagagg tcggtgcctg gtaattggcg agacgctcaa
    11281 tatatgtttt gattcggaga acatttgagt aacgataacc attggcgtat ccatgcgcac
    11341 ctgcgccgaa ggcgaggtag gggagcgagc gccagtattg aaggttgtga cggcaggaaa
    11401 aaaaaggatg agggatgaag gatgaaggat gagaaaaaga ctcgtctcta tcctttgccc
    11461 aattcgaaat ttcataatgt gcatatccgt ggctttccaa aaaatcttct gtaaattcat
    11521 acatttcggc ggcaagatcg gggtcaggga tggggagcag gcctttcgat gcccaacgcc
    11581 caaacggcgt gccgtgttca agggtcaatg cgtaggcgga gatgtgctcg ggctgtaatt
    11641 ccacaattcg ttcgagcgtg gtctgccatg aagtgagagt ctgttcggga aggccgtaaa
    11701 tgagatcgag gttgagattg tcaaaccccg ccttgcgtgc agtggacacg gcttcgatga
    11761 cggtgaaaaa gtcgtggatg cgttcaagca tgcgcagttc ttcggtgttg gtcgattgta
    11821 cgccgaagct gatgcggttg atgcctgctt tgcggatggc atccagtttt gcaggggaga
    11881 ttgtgccagg gttggcttcg atgcttactt ccgcgtcagc ggtgaatgtg aaggcggagg
    11941 tcaatgcttt tagaatggaa tcaaattgcg gcgcggaaag aagcgacggg gttcctcctc
    12001 caaaaaagac cgttgggaca ttgaatgtcg gaagttttac aagttgatcg ccaataaaac
    12061 cgatttcacg aatcagggca tccacataag cggggatcga atcttcctgg cctgcgtagg
    12121 tgttgaaatc gcagtaggcg cagcgatgtt tacaaaaggg aatgtgaatg taaatagagg
    12181 tgtctgtggt catgcgcggg atgtgtgctt tctggggagt ccgataaaca aggcgagcgg
    12241 gcctgcgatc agaatccaca tggcggagcc aatgccgaag gtttgtgctg cgaggccgat
    12301 gccgaaggga atgagtttac caaacaatcc tgaaacattg tcgagcacca gcgccgcgcc
    12361 gctttgacca tgcatggact cgaacagatt tgccttgaga atggcatacc agcccgcgtt
    12421 gaagaatccc atcatgccga cgatggtcag tttgagccat gcttgtgagg aaagcagaaa
    12481 cagcgggaag agaatgagct cgatgattac actgatgcga agatagtcga tgccttttat
    12541 gcgttccaca agtggaatga gtaaaaaatc tccgagcatg ccaaccccag tccatacgac
    12601 caccgagagc gcggcttggg ctggtgaaaa gcccgccaca tctacaaaat aaagcgggag
    12661 aaagccgtac aacacatcga gcatgagatc ggaaaactca agcaaaatca gccagcggac
    12721 aattccccaa tttttgagtg ctgcgaaggt ggatttgaat tgtgcaagga tcgccctaaa
    12781 gcctggtatt tcttcggtgg tggtttggtc ggtgggaatg cgtttccaca cgaaaagcag
    12841 gatcagaatc gcaatgcatg ccagcccaag gaacatgccg cgccagccaa aaccaatata
    12901 ggctgcaccg ctcaagagaa tggggccaat gaccacaccg agagagcctg caaaggtcca
    12961 gcgagccatg ttttgttcgc ggcgttcggg ttgcagatcc atcagcgagg cttgtgacaa
    13021 cccaacaagc gcgccagagg acgggttgaa aagaatgtag gaggatagaa ggaacaggaa
    13081 agaggtgctg attcccgtca gtgcaaggga aatgataaag aaaattccac cagtcagaag
    13141 gacgacacgg cgtttccatg catctgcaaa agtgaaaatg aacggttcaa taatattgcc
    13201 gaggatgccc ggcagggaga gcgcaaggcc gatttggaca taattcaaat gcagatcgtc
    13261 gcggatgaag ggccaggcgg cttcactgat gccataggta agttcatcaa gaaattcaat
    13321 cagcagatag gcaaagctca tatggtttcc agtgatgtgg ggtgtctttg ccctcaaatt
    13381 taacacagtt ccatgttttt accacctgtt ttgggcttgt gtaaaatcat gcatggaaaa
    13441 acaaattgaa tgacgattgt gaccctttca gcccttttgg tgacgatttt gatttcgatt
    13501 tggtgatgtg ataaagaata ttcacagcta aaaaatattg acactgcgcc gtgacatagt
    13561 ataaatgccc atacatagag tctgctctgg aacagtttca ctcgttacgc aaggaataca
    13621 tcttgcggat cgttttgaaa tgaaaaagat ggtgttcccg ggcgggttct ttcatttttt
    13681 aacccctgga ggtgtgagtg aacgcgactt tgaaattggg agagcatttt atttttgatc
    13741 tctccgactg caaccatgag attctcatgg atagcgagcg agcttattcg ctgtttgcgc
    13801 aggctgtgcg tgaaagcggc ctgacggttg tggatgaggg cttttacaag tttagcccgc
    13861 acggctttac ctgtttcttg cttctcgcag agtcgcatgc aagtttgcat gcctggcccg
    13921 aacataatta ttgtgccatt gacctcttta catgcgcaat cggcaaggat atgatgcctt
    13981 tattgatgaa gatcaaacag gctttcggcg cggatgattt ttcgatgcgc aagctcgacc
    14041 gcgaagcatc tgtcgaaacc agattgccga tcgccgtata aaaagaggaa accattatgt
    14101 ccgaaacaac aaaaccgccc ctgtattgga tcgccgatta cctgacccct gatgacgttt
    14161 tgcagcacgc catccataaa gtaattctgc ataagcacac cgctttccaa gagatgtgga
    14221 tcgtggattg cggcacttat ggcaaaggac ttgtgctgga tggcatctgg caatcttcca
    14281 caggtgacga ctttttgtat cacgaatctt tggtgcagcc cgcctgcgtg aaccacggca
    14341 accccaaacg cggacttgtg ctcggcggcg gggaaggggc aaccatccgc gagttgttga
    14401 agtggaaggc catggaacgg gttgtgatgg tggacctcga cggggaagtg gtggaagcct
    14461 gcaagattca tctccctgaa atgcaccagg gcgcgttcga cgacccgcgt gtcgagttgg
    14521 tcattcagga tgcagttgcc tatctcgatt cttctccatc tgaatgggat gtcatcatct
    14581 cagacctgac cgaccccatc gaatctgggc cgtctttcaa gttattcacc aaggaatatt
    14641 tctccaaggc gcaaaaagca ctgcgcccgg gcgggtattt tctccttcag gcagggccag
    14701 tttccccgcc ttcgatggat atgcacattc gtctggtttc cactcttaag gcagtgttca
    14761 agtatgtcca ttcgtatcac atccatgtgc ccatgctggg gcacccgtgg ggctttatcc
    14821 ttgcaagcga ttccccgatc aacacccgcc ccgaacctgc agaagtggat cagttgctgg
    14881 cagagaagac caatggcatt ttcaagatgt ttgatggtat taccctgttg ggtttgatgc
    14941 aatcgcccag gcacatccgc gatgcgatcg caaaagagac cacggtgtac acgcttgagc
    15001 atccgcctgt tttcagcaca caggaactgc ccaagtagta aaatatccat agtcttctac
    15061 aggtcaccag accgccgacc atgggccgta gatttcggtc cttcgtcggc ggtttttatt
    15121 aaaaggaatt tccatgagca tttggttcac agaagagctt cacccttact atcgcaaagg
    15181 catccgcatc aagaacatac tggccgatga gcagaccccg tttcaacatg ttcaggtggt
    15241 tgaaacagag tttttcggca aagccatgat tctggatggc atcattcaac ttaccgagcg
    15301 cgataacatg ggctatcatg aaatgatcgt ccatgttccc atgctggcgg ttggtcagcc
    15361 aaaacgtgtg ctcattgtcg gcggcgggga tggcggctcg cttcaacagg tcttgcgata
    15421 tccctccgtc gaagaggcca ttgtctgtga attggatcag cgtgtggtgg atctctcccg
    15481 agagcacttc gcttcttttg gcgacccttg ggctgatcct cgtgccaggc tgctcgtacg
    15541 ggacgcattt ggttacctcg aagaaaaccc ctcccaattc gatgtcatca tctcagatac
    15601 caccgacccc attggcatgg ccgaacggct tttctctgag gaattttaca aactcatggt
    15661 ccgcgctctg acgcccaacg gcgctgccgc cactcaatgc gaacagccgt ttttcgacac
    15721 ggaattgatc aagcagatcc atcactccgc caaaacgctg acgaagaacc ccgcttatta
    15781 ctatgctaat atccccacct atcctggcgg cggcattggc tttatgtatg tctcaaatac
    15841 cccgtgggaa aacggcctga aaaccccgtt cccgcctggg aaaaaccaat atattaaccc
    15901 tgaaatccac aaagcggctt ttgccctgcc cgagtttttc aggcgggaat tgatgggctg
    15961 attctcaaaa acccttgagc ttttcaaggg ttttataata cccacggtca caaattgcgc
    16021 tttcccgcct ttgaaaaggc ataatttgtg accgagtgcg gtatattttc aaaggttata
    16081 atctgcacag aggcatctta tgagcaaact cattgtttcc tactcacgaa aagattccgt
    16141 ggttgcccgc aagttgatcg atacgttcaa aaaggacgaa ttcgatgtct gggtggattg
    16201 ggaggatatt ccgcctgcgg tggggtggct cgaacagatt ttgcgcggca tcgaagaatc
    16261 agacgcgttc attttcctca tcagtcctga ttcggtcaat tctgaggtgt gcaaggtgga
    16321 aattgagcat gccgccaaaa acaacaaacg catcatcccg atcgtgcttc gtgaggtgga
    16381 ccccaaaacc gtcatcccca ccatccgcga tctgaattgg atcttcattc gtgaacagga
    16441 tgattttgcc gctggcattg aaaaagtgaa ggttgccatt acgctggata tagaatgggt
    16501 ggaggaacac cgtcggctgc aggtccgcgc cctggaatgg catcgtgaga aggatcccag
    16561 tcatcttttg cgcggccacg acctgcggac cgcccgtcag atggtggctt ctgctgaaag
    16621 caaagacccc aaacccaccg agctacagcg tacctatatt aaatacagta tcaacgatga
    16681 gacacggcgg acaaccttgt ttatttcagc cgctaccgca ttgctcgtca tgataatcct
    16741 ttcgataacg gctttttatc aatcgcaaaa agcttctgcc aacgagaaac ttgcccagga
    16801 acaaaaactg ctggcacaaa ataacgaaca gcttgccaaa aagaatgccg aaaccgctga
    16861 aaaaaatgcc gccattgctg tgaagaatca ggaagttgcc gagcaaaatc aattgatcgc
    16921 tgaagcacag cgcagcgcgg cacgcgccca aatttatcaa tcgcggacgg gcggtttgta
    16981 tatcagcacc ctgttggcgc ttgactccat gcggcgtagt ccatcgcagg aagcagagga
    17041 tatccttcgc aagaacatct cgcttctgcc tgtgcctgtt gcgcaaatgg agcaggacga
    17101 tatcatcaac gcgctggaga tcagctcgaa cgatgatgct tttgtcactg ccagtgcaga
    17161 cggaacggtc tgcgtgtgga atcttccaga tggaaaaatg cgtttttgcg caaccagccc
    17221 cgaggctgtg gaagatgcgg cttttagtcc cgatgggaaa cttattgcaa ccggagatac
    17281 ctctggtgtg gttatgatcc ttgatgcgca aagcggcgag attcagaata cctatgaata
    17341 tggcgtcccg atctgggatg tgaacatcag ccccgatggg aaatctctgg cggccgcccg
    17401 tgacgatggc cgtatcacat tcgtctcgct tgtgaatcgt aaattttcat ttgagctgat
    17461 tacgtttggc ggcttgtatg tgaccgcctt cagccccgat ggcacgtgga tcgcggcggg
    17521 cagcaatgag ggcaccatca cattttggaa tttgggcaac ggcaaggttt tgaatgggcc
    17581 atcgcaccat ggtgaggttt ttgaaattgc cttcagccct gatagcaacc gacttatttc
    17641 aggcggttct gatagcgtgg cttacatgac ccttgtcagc acgggtgagg agttgttcaa
    17701 gatcatcaat gaagattggg tcgaagacct caccttcagc cccgatggca aatggtttgt
    17761 tacggtttcc gatgaccaaa gaatcagggt atgggacacc gctacgggca aagaaaagct
    17821 gcgcatgttg caggatagtt tcctcagcag tgtggaggtc agcccagatg gtcaatggct
    17881 tgccacaacg ggatacgata aaacagtgcg ggtgtggaat gccgccacgg gtgcggagat
    17941 gtttcaaatt ccgatcaacg ccaatggcaa catgcttgca tttagcaggg atggaaaata
    18001 cctgatctca ggcgaccagg ctggcgagat caatgtttgg gatatttctg cattgcctgc
    18061 ctccagtagt tacctccaga tgggtgcgct ggcatcgaat gttcaataca gcccatctgg
    18121 cgaatggatg gtcgcttcag acgggaacga agtctggttg ctcaccaagg agcaactcgc
    18181 cgcccaaagc ggactcttga aaggctctcc gacctttgaa atcgattccc tcattcaaga
    18241 tctcattatc agccctgatt caaaatgggt cgctgtcacc actgacgatg ccgaagtgat
    18301 cttatataac gtagcttcaa aatcgaagaa attggttaca gaatttaatg atgtgccatc
    18361 catcgttttt tctgcagatg gttcccagtt gatcacggga gattatgatg gttctgtgca
    18421 agcctgggat acagcgagcg gtgccctggt tgcagatttg tttgataccg atcagaatgt
    18481 taattttctg gctataaacg ccgggcaact ggctattggc atgcaggatc aaatcgtcat
    18541 tctggatgct gctactggcg aagccatctc cacgatcgaa tctccgggcg atcatcaatt
    18601 actggccttt agtgcagatg gttccctgct tgcggcgaac aacacttcag gtcaggttta
    18661 tatctggcaa aaacaggcgg gtggttttga tttacttcag aacattccaa gtgaacaggc
    18721 cgcatccatg atcttcaacc caaagggtga ccgtctcttg atcggtgcgc tgaacaatgt
    18781 ctttgtgctc gatccattta ctggcggcga gatcacacgg attcgccaca aagataccgt
    18841 caacagcatg tcctattccg tcgatggagt tactctggcg acttcatcct tcaaggcgat
    18901 tcaattttgg gatatgcaaa aaatgtcaca gcttcaggga gatgacctga tcaaagcggc
    18961 gtgtaaccgc ctcacccaaa attttgacac tgcccaatgg accacttttt ttggtgagga
    19021 gccttatcag gtgctgtgtg agaatttgcc tgtgccgtag gtgagtttgt ttttcttgtt
    19081 ttgacataat tgttcctgct gtatctttgt cacaatatgg gttattgttt ttcaaatagt
    19141 ctatcaatca tattggatga ggccttattt tgctggatct aaagactatt tttgctggtt
    19201 atattcttag taacgcaata tgtgcggttg tgatcgcatt gctatggcgg cagaaccacc
    19261 gatctttgaa tggcctgggt ttctggctgg ctgattttgt tttgcaattt actggtctgg
    19321 tattggtcag tttgcgggaa atactgcccg attttttccc cgttattgtg gctaatacca
    19381 tggtgatcgg ggggacgatt cttttgtata ttgggctggt acgttttctt gatacaagac
    19441 gtccccagat ccataactat atttttcttg ccctgtttgt cagtttgcag gtttattttc
    19501 tggaaatcca cccaagcctg gaagcgcgta atatcaatct ttcggcgggt ttgtttttca
    19561 tttgcattca atctgcctgg ttgatgctgc gaggtaagaa aattgtcagc ccgcctgaac
    19621 gcggggtggg gcttatcttt ttggcatatt gttttgtgag catggtccgc atcatgacgt
    19681 atgttgtaga ttctcccgga gagaactttt ttgccgaggc cactgcgttt gatgccattt
    19741 ccatcctgac ctaccagatg ctttttattg cgctcacttt cagtctggtt ctgatggtga
    19801 accagaaact ggctgacgaa gcagctgcca gcatggatat gctccataat tccagggaga
    19861 tcctgcgctt tagcgaggaa aaattctcga aagccttcca tgccagcccg gatgcgattc
    19921 ttatatcacg tgcaagtgat gggaagatca tcgaggttaa tgaaggtttt tcccgcatgt
    19981 tgggatattc gcatgaagaa gcgttgacca aacataacct gatactacaa ttgtgggccg
    20041 accccaaaga ctgggaaaat gtgttgggtc tgattcgaga gaatgaccat gtccataatt
    20101 gtgagtatgg gtttgtcacc cgctccgatc aaaagttata ttgtctttat tcaggggaat
    20161 acatcaacct cggcggggaa acctgtattc tttcaattgt gcgtgatatt accgaacgca
    20221 ggctggttga ggcggaattg cgggagagcg aaatgaaata ccgcctgctt ttcgagacga
    20281 tggcgcaggg ggtggtgtat caggataaag atggacagat cacctttgcc aatcccgcgg
    20341 ccgaaaaaat tctcgggttg acgcttgccc agatgcaggg acgagcttcc atagattcac
    20401 gctggaacgc cattcatgaa gatgggacac cctttccggg agaaacgcat cccgccatgg
    20461 tggctttgcg ggatggggtg gctgtgttca atgttgttat gggcgtctat aatccgcaga
    20521 ctgacgaacg tcactggatc aacatcaatg ctactccgga attcaagcca ggggaactta
    20581 ccccatatca ggtctatgct acatttgaag atattaccca gcgcatgctc gtggaagagg
    20641 aattgcgaaa gaccaacgaa ttgctgcatt ctcagcttga tgaaatccag tcattacatg
    20701 atgccctgcg ggagcaggcg ttgcgcgatc ctttgacagg actgcataac cgtcgttacc
    20761 tcaacgaaac catggggcgt gagattgccc gtgccaaacg ggaaaaatat cccatcagca
    20821 tcatgatcgt ggatgtggat aattttaaac aattcaacga tacgtatggt cacagccttg
    20881 gcgataaggt gttggtggca tttgggaaag ttttgcatga gaatacccgt gttggcgata
    20941 ttgcctgccg ctatggcgga gatgagtttc ttgtcgtgat gccaggcgca gacataatgg
    21001 gtgccagtca gcgggctgaa gatattcgtc aaaaatttgc ggctactccc gtttattatg
    21061 gcgatagcga gatttttgct acgatctcga ttggcctatc cacttatccc caacacggca
    21121 ttgacagttc cctctttcgc tcggcggatg aggctctcta tcatgccaag cactctggac
    21181 gtaatcgtat ctttatctgg tctgagaaaa agatgtttgg gtaaacnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnaaca atatttcaag cttatggagc gattgtatgt
    21361 tggtgcggga tctgatgacc atttatccag tttcaatttc catccattcc gacctgcgca
    21421 gggccgcaga gatcgtcagc ctggccgggg ccagtgatct gatggtggtg gatacggaac
    21481 gtcgtttttt aggtgtttta tccgaaggcg atttgatccg cgcgatcatg ccaaattttg
    21541 atgaagtgct ggaggcaggc gggacacttg atgatgcctt caatttcttt atccgcaagg
    21601 ggaaagacct ggcttcacgc ccgatcgagc cattggtcat taagaacccg atcgtggtca
    21661 gcccgactga tgaagctgca caagtagcca ctattttgat ccaaaaacag attcgcagat
    21721 tgcctgtcgt ggataatcaa cttctgatag gtactgtttc acgtgctgat atttgccgcg
    21781 ccgtgattta ctctgcgtaa catttcgtcc catttaatat atgcttcctt cccgattggc
    21841 atttgagaat gccgtttcat tcaaccaaaa gcatggacac gaaaaccttg gctttgtctc
    21901 tgctcaatgg ggattcctgc ccgtcaagcc gccattgttg gccctgcctg attatttcaa
    21961 ggcgtgggat gtgatcgccg ctgatctacc ttccttgttt cgcacacttc aagtgcgccg
    22021 tgcagtagat gccttgccat atcttaatgt ggatgaacat cacctgcctg ctgagtatct
    22081 tttgcgtgca tcttcattgg tcagtattct gttacatgcc tatgtccgtg tggaggcttc
    22141 tgcgcctgcc acacttccga gccatttgat gcaggcttgg gaggggattt ctcaacgcct
    22201 tgaccgcccg gctcccatcc tttcatacgt tgatttgatt ttatataact ggaaattgcg
    22261 tgacccgcag gcatctcctt tgatgctggt tgaaaatctg gatttgttgt ttcccacggt
    22321 cggcaacgaa gaagaacgag tcttttatct gacgcaattg gagatcgctg ccgcctgtgc
    22381 tccgcttatg cttgcagtga ttcgtgcgca ggaagctgcc agccgcgatg atgtgcctgc
    22441 tcttgagcgc gaattgcacc ttatgttgga tgtcttcagg catgccagtg aattttcagt
    22501 ccataagatt gacccaaatc catacagccg cacgtatgtt gaccaggtcg tttgggcaaa
    22561 aacggtcgct ccctttgctg tgccattcat aaaaggacgg gttggtccca gcggcacggc
    22621 tgcccccatc tttatgtaca tggatgcttt tttcggtcgc gagtctcatg cttctacatt
    22681 gggacgcgaa acagaaaacc tgctgaccta ctcccctttg cattggcgta cattggtgga
    22741 agcggttggt aaattctctg taagaaacta tattgcccgt tcagggaatc gggcacttca
    22801 gggtctgttt gatacactgc ttgaagcata tgcaggggat aaaggttatc tgggtacgca
    22861 ccgtcttaag gcatatggtt ttctggaaat tgcctttaaa gcaggccgct cgctcaccat
    22921 tggcggattc aagggattat ttcgtgacaa aacctggaat cagatcgatg atgaactcgg
    22981 acatgcacgt aatgaacgct atcttggatt aacccctcat ctatactccc ttcatcttga
    23041 aaatggtgag agttcgttcc ctgtctcaag gaattggatc acttctttaa ccctggaaac
    23101 gcgtagcgat gagatgcatt accgccctgg cgatcgaatt agccttttgc ctgagaacag
    23161 tcccgaatta attgatcgca ccttgaaagc tcttcatgcc gttggcactg aatccattca
    23221 tctcaaccat atgtggcagg cccatcttca attccgcagt ggatactccc atgaaacaaa
    23281 cagcctgcca ttaagcgacc tgttgcgttt tgggcagata cgtcctgtaa atcgtgcaac
    23341 tgccaaggcg ttgtttaaaa taacaggctc cagttttctg ggacgtattc ttaatgcacg
    23401 gatggaggat cagtgggaat tatgggattt gcttcagatg ttggaaagag gcggttttga
    23461 tccgcggcaa ttatggaagg cagagccgtg ggaacccgaa catatcacac gattggtgag
    23521 gcctgaatta ccgcgtctgt attccattgc ctcttcgcca agtctcaaga aaaaacttga
    23581 gatcagtgtt gcagggttgg aatacgactc tgtggaaagc cctgtatcca atcaatttcc
    23641 gcggcagggt gttgcctcca gtttcctgcg tcggttggcc gagcaggctg aaggtcaaac
    23701 cgaattgacc gtgagactta gtccatccgc acgttttcat ttgccagcgg atgcagatcg
    23761 ccccatcgtt atgtttgcag gcggttcagg gattgctcca tttgcgggtt ttctacaaga
    23821 gcgcatgcag tccgatttgt cacgtaactg gctgtatttt ggtacacgta ccgaggcgga
    23881 aattcatcac cgtgacctgt tggaaaaact ggcgggggca ggccgtttgc atttgcgtgt
    23941 ggcgctttcc cgtgaggaca aacagatctt tttgcaagat gggcgtttgg cggctcgttc
    24001 tggtactgcc tgtcatgtag attctttact tgaccaggat gcagagaccc tatggagttt
    24061 gcttcgaaac tccgatgaag gcggggaaca agcctgtttc tatatatgtg gacgtacagg
    24121 ctttgctgga agcatcattt ctgcgctgcg caatgtgatt cgccgttttg tagactctga
    24181 ttcaaaggca aatgatttct ttcgccaact gatggcagac cgtaggatca tgctcgatat
    24241 atttacgacc tatggcggga cgagcgatca ggcgggtaga gttttcgata tctcggatct
    24301 ggttttgcac aacaatgatt caaatggata ttggatggcc ttgaatggca aggtttacga
    24361 cctgagtgaa tttgtacatt tgcacgcggg tggaaggcgc atccttgcca acaatgcagg
    24421 gatcgatgcc acccgtgcct accaatcggt tcaacatcac ctcaatagtg atgtggatgc
    24481 gcaaacagga atgtatgaaa tcggtcgtat gcggcgcctg gatttaggaa tgcgatgggg
    24541 ggttgggctt ggcttgcagg gattggtctt ttttaccctg gaagaggcgt tcactttttg
    24601 ggtccgttat ttatatctgg taaccgaaat ggaaaacgcc ctgataaatg aattcagttt
    24661 cttggaccgt tccacaaccc tgggggaaga tccgcgagag tttacgcccc ttaaggcgca
    24721 tcttgtggct gaagcccaaa aacgttttct cggcactttt cttgatggga ttcttggtga
    24781 agacctgaat gtcttgtggg cgatcacggc agggctttgt gaccgccgaa gtcattacaa
    24841 ctggatgccg acttccattc aagatacaac gttgagcgca agtgccctcc atgcaagggc
    24901 caattacctg cagacttgga tcgatgaatg gcggtcacag gatgaagagg ttcacaagac
    24961 tcgtttcgag caggcggtta cccaattaag ttcaatcatt cgggctgaaa acctgcgctg
    25021 ttttcatgaa atcaaactgg ctgtgcggaa tggtgtgcag gcctttgaaa ggcatgaagc
    25081 actcgtgatc gaaaaggatg gcgctggatt gatatcaaca cttcagcaaa tccccgttat
    25141 cgtcagttcg tattacgaac gtttgggggg agagatgcaa aaactggcgc aagaatatgg
    25201 ggttcccctg aaatccgttg taaattccgc acaggtggat tttcaagatc tcgggcacgg
    25261 cgcgcacatg gacgtaactg ccaaccctcg gatcgacacc gatggctgat tagttttatc
    25321 gaagccaaat ataacctgtg cgccttcaaa cctaaaaata tagttgggtt tatgccgcat
    25381 gctttgtcat agcctgccag gcgatctcaa gatccgtttc tgtggtgcgc cagttactga
    25441 acgcggcacg aatggcggga gtccccatat atgtagtagg tgtcataaat acacgcccat
    25501 catcacggag gttggccagg aaagttttga ccctttctgt atctacggca tcgtgaaatg
    25561 taaaacaaac cacgttcatc cgtacggggg cgagcagacg aaattccttg ctggccgaaa
    25621 ttttcatgcc aagttgctct gccatttcgc aattgcgttc gacgatatca cgatagcctt
    25681 ccctgccata tgccatcaaa ctgaaccagg ctggcagggc gcgcatacgg cgggagtttt
    25741 ctggggagag gtgaataaaa tccacgggtt cggcaattgc accgagatag gctccactat
    25801 tttggaagac cgcaatttgc aagtcacgat gctgggtgaa gatcatggct gagtcgtatg
    25861 gcacattgag ccatttgtgc gcatcaatgg tgatgctatc cgcgtgttcg atgcccttca
    25921 ataaatgttc gaattttggc gagcaggcgg caaacccacc aaaggccgca tcgacatgca
    25981 gccagaactt gaatttggat ttcattgcgg cgatcgcttc aatgtcatca aaatctacag
    26041 tgttgaccgt ccctgcattg gcaatgacga cgcagggctc gccgttcaat tcttccaatg
    26101 cttttcgcaa ctcgatgaca tccaccgctt ctctgttttc gggcagtctg gggatgactt
    26161 tcagggagtt tcgtcccagc ccaagcattc ccaatgcctt gaacgaactg gcatgtgctt
    26221 cacaactcag gatcctcacc gggggaatcg cctgcaaacc ttcctgtgaa accgattttc
    26281 catgttgacg cccaatccat tcacgggcga tggcgagacc agcgaaattg gacatcgtcg
    26341 caccagtgac aaatgtccct gagaaagagg cgggtaatcc aaacaactca cgcagcatgg
    26401 agatcgcctc cacttcaata tccggtgcca cagagttccc ggcatggctt aagttcaggt
    26461 catagacgga ggtcaaccaa tcaccaagca gggaagcagg agttgtgccg ccagtgacca
    26521 gcccccagaa acggggtcca ttgctggcag gcatgtgatc tccaaatcgt tcgagaaaca
    26581 tttgcagtgt tttctctgca ccaatacctt gaacggagat cttgagattt tcttttttgg
    26641 aatacgccgc tgcgggaggg cgtatggcta attcgcgaag ataattttca gatgcttgcc
    26701 ttgtctgctc caataaagaa gataggttgt cgagatcgtt cttcaactga ttgttcataa
    26761 acacctcttt ttcatagtat atcaactgaa taatatttat aaaattccgg gggagatttt
    26821 tcaatgttgg cgcgtctatg gggtaggaga aaacccaatg acctttcaat tttcaattac
    26881 acagttcgaa aaagtgagaa gctgaccatg attccacacg aaaactcacc cgtgtacgcc
    26941 gcaggtgcaa aactggagaa tgcttccgca gccatgatcc tcctgcacgg acgtggcgct
    27001 tccgcgcagg atattctttc acttgcctcc catctggatt tccccgggat tgcctatctt
    27061 gctcctcagg cagaggggtt catctggtat cccaaccgac tgattgctcc tgttgaatcg
    27121 aatgagccgt atctctctgc tgccattgcc aaaatagacc gcttggttaa gcaggttgaa
    27181 gccagcggca ttcctgcaga aaagatcttt atcggtggat tttcgcaggg agcaagcctt
    27241 tcaagcgaat ttgtgatccg taacccgcgt ccttatggag ggctgcttgt tttcagcggc
    27301 ggctacattt ggcagagtgg aaaaccccgg gaaacttcag gcgatttggg cgggatgcct
    27361 gtctttatcg gctgctcaaa tgtggacccg tttatcccgc ttgaacgtgt aaaagagact
    27421 tctgctttgc tggcatccat gaatgccaat gtcactgagc agatctatcc gaacatggac
    27481 catactatta ttgacgagga aattgaattg gcaagacaat tgttggaaag acggttgtaa
    27541 accgaccttt ctttacttga aaaactgcgg cggaattcgc cgcagttttg atttttgatc
    27601 caatatctgg cagagccaac agaaataagc cagagaatac tttcgatgct ctttaaatga
    27661 tacacttcgg gccaaaaata ttctgaggta agcgaggaat aatcaccatg cctgaatttg
    27721 ctaaacgtat ggcttatatg cagtccacag cagatgtgat ccgatacttg tttgaatcga
    27781 tgaccgaccc ggacgttatt tctttcggtg gaggcgctcc tgcaggggag gcattgccgg
    27841 tagagttggt tcacgagatc gcaaaagaag tgctggtccg cgaaaaacgg ggagttcagg
    27901 cgcttcaata tggaaacccg ataggcatcc cagatttgcg gcaggctgtg atcgaccatt
    27961 tgttgctccc taaagggtta cgggttggca tggaaaatgt catgatcgtg gcgggcggaa
    28021 tggaaaccat gaatttggtc agtcagttgt atttggagcc cggtgatatc gttctggtgg
    28081 aatcgccgac atttcttcaa tccattcaga tattcaaaat gttcgaggcc cgctgcatcg
    28141 cctgcgagac agatgatcaa gggttggtca ttgatgacgt ggaaaccaag atcaaacaat
    28201 atcatcccaa gatgatttat gtcgttccca cttttcaaaa tccctcaggg cggaccacca
    28261 gtttagagcg tcggaaagca ttggcccgtt tggggagccg ttatgatatt gtggttttgg
    28321 aggatgatcc ctattgcgaa atgaggtata gcggggaggt gctgccgccg atcaaatact
    28381 ttgacgaaac tggaaacacg atctttgcga atagcctttc caagatattt gctccgggca
    28441 cccgtcttgg ctatattttt gcggatgcac ccatcattag gaaattttac gatgcgaaaa
    28501 ccgccaccaa ttcccatacg aatgtggtta cacaaatcct gtgcgccgag ttctttaatc
    28561 gcgggttgtt cgcgccgcac ttgcaagcca tccgcaatat tcaccgtgag cgccgcgatg
    28621 taatgatgga ttgcatcaag tccatgctgc ctgcagaggt gagatctatt tatccggatg
    28681 gcggtttgtt cacatgggtg gaactgcctg aacacgtgga taccacctcc atgctggaag
    28741 aagccaaaca gcataaagtg gcctacatgc caggcaggga attttttgta gatgggcagc
    28801 ccatccgcaa taattgtatg agggtcagtt ttgggggtgt tccccccgag aaaatacggc
    28861 ttgggatgga acgtttggct gaagtgatct gttcgaaact gtaggatttc aaaataaaaa
    28921 cttccgaagc cattggcttc ggaagtttgc ttttatacca acattctgac tggattttcc
    28981 aggaactcgg ccattttctg catgaactgt gccgcttccg ctccgtcgct gacgcggtgg
    29041 tcaacggaga tggtggcttt catgcgcgag ccgatcttga tctcgccgtt cacgaccacg
    29101 ggcgtttcct tggcggagcc gatggcaagg atggcagcct cgggcgggtt gacgatggcg
    29161 atgaattctt ccacgtcgta catgccgagg ttggagg
//
LOCUS       DAOQLC010000048        28902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_517_length_28902_cov_21.432281, whole genome shotgun sequence.
ACCESSION   DAOQLC010000048 DAOQLC010000000
VERSION     DAOQLC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_28902_cov_21.432281"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="PL000_10205"
     CDS             complement(<1..2270)
                     /locus_tag="PL000_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNC89317.1"
                     /translation="MNSNKRTRIWSNPLMTAAYSETPIFQNREQEQRERIALTVARAF
                     LVTSTIISFSILYIAISLNSWQLFTLYGGNILYILLTYAGLQQLQKKNTLFGIFLVTG
                     SVHLTIILLPILFEGIATLAGLFSTLAAALILSQTLETRVARGAIVVSNITGIGIILL
                     GSIPFPVRIQIPSVLVVTIAILAIIETAIFMVLIWRQFRTFNIQTKLTLVFITILVTS
                     LLVTGGYNAVQSYRTQRAQTANNLENELGVKRSVIIDFLEASRQDVVFLGEAEVVQSY
                     VKTIDDIAAPNIVVRARTIMEREFRRFAESRGIYQEIRFLNNDGNEVIRIDTALDGNS
                     SIVPQAELRQKGELDPARELSYFAQTITLPAGRLFQSPINLRKLDGTIVTPHEPIIQF
                     GVPLVINTQTKGVIMANIYAEKFLGILSASGDDTFLVDTNGYYIAHPDESKRWGNDLN
                     NNITIKNDFPDLVNNLNNGNSGSMESNGYLVIYTPITIPGETEPRWYIGSFVSIDSIT
                     KPIFESTYNALSLLLLTIIASIFIMSTLSNSITSPLGNLALAAQDVAAGKLSARVNLQ
                     TNDEIGALASIFNSMTDQLRETVSSLEARVADRTAELAKEKQQSDTRAKQFEAITKVA
                     RAISAKSNLQDLLPQISRVISEQFGFYHVGIFLNDPANHMAVLSAANSDGGKKMLQRS
                     HQLRIGEQGIVGYVAQTGNPRIALDVGEDANFFNNPDLPDTHSEMALPLKASNKIIGV
                     LDIQSTMTAAFTDEDF"
     gene            complement(2236..5055)
                     /locus_tag="PL000_10210"
     CDS             complement(2236..5055)
                     /locus_tag="PL000_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89318.1"
                     /translation="MNVKNTSDQQEEARLKRNALGISLITGILFLVVSVFAVIVGYQE
                     NGMNGLWGGAITGFISLAAFFSSIQAYRGRTSIGIGFLIFAILLVSFAIPIVAQGHGL
                     ALSILVLVVVASISSTTLSAKWTTRSIIIAFIVAAAIILIDLYLPDLGLPGNPTYINI
                     VALVTSLAYIAFILRSFNTYSLRTKIITAFTLITITPLVTLGYFYSLSSTRILENQSK
                     LQLTNIAKIQAESVDNFITNQIDSVHADAKQLAFVEYLRLPAAARSGSVKELNARLAL
                     LALTRRDPVFIHSIAILDTDGTNLIDTFEDYKGQTENSLIYFVRPMQTGLPFASNILF
                     QNNRNHIYFSAPIKSQNGNTIGILRVEYHATIIQAIVRSVDPLNAGTTLSLVDTNTYL
                     RVAYTGDRDELFKTYKDFSAPDLAVLQSEGRLPSISIESLLTETDVAMVDGIDNLQQQ
                     SFFDATSKSLGSTTNTGVFLETQPWVAIVRQSKSNYLAPVEDQNRTTILISLIMVILS
                     IGAGFLASQVITSPLLTLAKTAEQIAVGNRSARAEAVTEDEIGLLAKSFNRMTDELNQ
                     AFKNLEIRVVERTTDLEIARRQSEKRASELQAIGEISKVITGEQALEKLLPLISRLVS
                     ERFGFYHTGIFLLNETNQFAVLQAASSEGGQKMLERGHKLEVGESGIVGYVAKFGMPR
                     ISLDVGHDAVFFNNPDLPNTRSEMALPLKVRNRIVGVLDVQSEKPGAFTEYDASTLSI
                     LADQIAIALENARLFTQNQQALAEAQAQYRQNLQSSWRVFSREEETIGYHQGLMGGKK
                     ISKPVETEEIREAINRGNVLVLNSETDKREATIVVPIKLRGQIIGTMNIKAPSANRTW
                     SRNEVNLAEAISERLSLALENARLIQESQRQVIKEQTISEVTGKIGASINLKSVLQTA
                     VEELGHAIPGSEVLIEFEQKNENME"
     gene            complement(5083..6051)
                     /locus_tag="PL000_10215"
     CDS             complement(5083..6051)
                     /locus_tag="PL000_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC89319.1"
                     /translation="MRTPTSKFLPVLLLTVIFSACTAISQPVSAPLPPLRVEFTNWWG
                     DYTLLVAQEKGFFEKYGVQVEPVYYEFFSDTYPDLAAGQLDAGLIAVGDTINISRIAP
                     MKVVAVHDNGGDDAMVARPEILSIENLRGLTVGTQIGSQYELMITEMLRSANMDIADV
                     KVVSIYPEDGLAALENNQVQAVYIWEPYLSEALSKGYKVLYPQEQLHLFPDLIVFQKS
                     VTDQRPDDVRAFLKAWFEAVDYRLQNPEETRAIAAKYLGVSVEEVQPDDNLKILTLED
                     NKAIFRLESENSIYTVTKITSDFLIAIGTITQMPDPLELLDSRYLP"
     gene            complement(6070..7800)
                     /locus_tag="PL000_10220"
     CDS             complement(6070..7800)
                     /locus_tag="PL000_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89320.1"
                     /translation="MLLKIINYFRDEEDSDPSFIRLVRNIVIFVLVVNIALLPLVTGI
                     VGEGSRNPRAFFTLLTTLILEIISLFLVTRGKLGMAKVVVPFALIAAVTIISLASNGL
                     KNAAMVGMPIILVISAILLGRRSIYLTTPVAIIAVTIVAVANLQNQIPIIPAGVDDAI
                     ILPVLLLGTAAIVQLLITRLNDSIKRARESEQIQKTENAELNELRSMLEERVNQRTSE
                     LEFANRNNERRARQFEAISQVVRVVSSIQDLELLIPRIAQVISEQFGFYHTGIFLLDD
                     EREYAILRAANSEGGRKMLERGHKLQVGQTGIVGFVTATGQPRIALDVGSDAVFFDNP
                     DLPNTRSEIALPLRYAGQIIGALDVQSTEANAFGQNDVEALTTLADQISIAINNTQVL
                     EESRKAFAESQSRFGDAVLESWKIMQPESLGLGYQLVNSSIKPLDVPLNGDHIQEAIS
                     QEKPILDNPEGKVSQLAIPIRMRGKIIGIMNLSSKNDYVLTKDDADIAEAVSERLSLA
                     IETATLLRTTQHRADIERVTADISSKISSSTHFDAILQITAQELSKALGGSDVLVQIE
                     PVSMELGMSS"
     gene            complement(7819..10779)
                     /locus_tag="PL000_10225"
     CDS             complement(7819..10779)
                     /locus_tag="PL000_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89321.1"
                     /translation="MMNRDTNMERASNSQQVDSVLVRNAYTISIMLLITAIPSVGLFV
                     YYALLKGSAQILTISIIMFIASFGAVAALFLIRRGRSNLAMMIIITSFTITFVAIPFF
                     IKGLGVILAISMFILAVSITGLAMPTRYAASGLIVGLSSGALILLLDYLFLGEARVRV
                     PELERATVFIVVGFALFFGAFTVRHFNRFSLRIKITISILLTGGVIVVTLVWFAASRT
                     GNIINTLTTRYETASRENIQNQLLHTVEDRAAQTNGFFDKLQNDLGILAKYRASLEQQ
                     KDIFIDSTYWDADNKVIQLTEGQYGNSPADVASIFIPSTVVVDESLYNDLNTTAYLDF
                     YAPNFLQTHSSVIALYFINTAGATTYYPNIRLAENVPADFDPTKEIFYTIASPENNPS
                     REPRWTDTYEDPAGNGLITTLSIPVYQNDTFLGVIGLDVQLAQIERAIANVKVGDTGY
                     AFLVDKAGHILSMPPEGYLFYNISPEEVTTNSVHELSVLSKGPFDVQEATAQMINGDS
                     EIATIKNGDGNSFFIFSPVTVPNYRLGIVAPENEFTTEFVQSRSEIEDIVQSALQGVA
                     IILIVLLVGAIIVSLWIGQLITRPLVRLTQTAEKISQGDFAARALVETGDETGVLAKA
                     FNSMAEILNSTLLGLEKSILERTRELEEANQSIIRRAGQFEAIARVARTISSTQTLNS
                     LLPQIVDTISQEFGFYHVGIFLVDAHREYAILAAANSEGGKRMLNRNHRLLVGGTGIV
                     GFVTNSGQPRVALDVGMDAAFFNNPDLPDTHSEIALPLLIGTQVIGALDVQSRETDAF
                     SQEDVSILSTLADQVSIAIQNARSYQQSRDALDQAEAASMQMSELQWGRFFAEQPSNG
                     FVFDGLDAKDIKQVKTKDENTYTIPLILRGTKIGTLKLNTDNPNRTWTDDEIAMAQAT
                     AERTAFAIENARLLQEAQKRAAKERAIGQITAKIGGLINIESILQTSIQELGNTIPNT
                     DIAIQISPEELEQK"
     gene            complement(10769..12496)
                     /locus_tag="PL000_10230"
     CDS             complement(10769..12496)
                     /locus_tag="PL000_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89322.1"
                     /translation="MSSNWFADLEDTNPSFLRMTRNILVFTIIANLAILLLVVGLFFR
                     ESQNAASSVALSVTLVLEIASLIFVLRGNPIPAKIVVPVSLFVAVTYTAINANGLHDL
                     SMLGFPTVIVVSALLLRKRALIISTPIAVLCVEIVAFADMTGITNSEMALKTDVSDAL
                     IAGILMIAISFLLQLLITRLNESAEEARNNAALQVEANKELLSLQANLENRIESRTRE
                     LNEVNAFNERRAANFEAAAQLARTVASIRDINKLLPAIAEVISARFDYYHVGIFLNDD
                     TQEYTSLVAANSEIGKQLMAGGLQYKIGQAGFVPSVAQTGYSRVSQDHNQIKSVSDSQ
                     EFQKTQSELVLPMKIENKVLGVLDMHSDKTYAFDEVAIEILSILADQTAIAIQNARAY
                     EETQAALAESQILYGTVIKQAWQTNVRVENKLGYRFSGTIPMLLEKPVDSQELQAAFE
                     SGKTVILAPEDQNRADGSVLAVPLILRGETVGVIHVKTPADIDMGEDEADIVRATAER
                     VVLALENSTLLEESQRRALREQTIGQISSKISAGTEIEMILKTAVRELGSQISGAQIS
                     VEIGSDNDE"
     gene            complement(12515..14215)
                     /locus_tag="PL000_10235"
     CDS             complement(12515..14215)
                     /locus_tag="PL000_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89323.1"
                     /translation="MNQPSENLDFSYTRWQASFLQATLIGACVFGLLAIIPAVLGTAD
                     PILIGIYIGAYIALLLITILPAPYTIKAVTLVGLLFLLGISGLLETGIRGDARLFMLA
                     AVTMATLLLSWRAGWIMTGLSMASFIIAGWLIVTGAITISSKNVTPGNFDTWISAITA
                     ALLLEVVIVNGIRLIQVEFESSRNRVRESFQKIEEERNLLEDRVKQRTQDLGRINRIS
                     EHRAQMFQTIAQVTRAIISTQNLQDLLPQIAQVISQQFGFYHVGIFLLDSNKEYAVLS
                     ATNSEGGQRMINRDHKLRIGQTGIVGNVAGTGKPRIALDTGLDATFFNNPDLPETRSE
                     IALPLFRSGQELIGVLDVQSTESNAFTQDDIQILTTLADQVSIAIANARSYEETQTVL
                     LESQLLYRRNIQAGWSKFVRSQKLSGIRKQGLKSSLLFDPMEVPGAKEVVRSGTIYKI
                     SEDDQNTSLTIPMKLRGEVVGLLNIKTEDENAIGTDELDIINAIVERAALSIDNARLL
                     AESRKTAEKERVIGEISSKVSSFTNRDNILQAAVAEIGRALPGAEVVIQLQKKNDTDS
                     "
     gene            complement(14252..16597)
                     /locus_tag="PL000_10240"
     CDS             complement(14252..16597)
                     /locus_tag="PL000_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89324.1"
                     /translation="MSQNTPAQTTKTFTGRENNLSLQTRLVLFVLFIALVPLTIIAVR
                     DNLQTQQALTNSAEISLQSSAEQTANSLDTFIQTTLNSIVIESQFSVFTEYLSLPNSQ
                     RITGRPEQFRAQGLLNKLSSKDSQNIISYAILDVAGNILLDSAPQRAKKNESDEAYFP
                     QVRFSTQPVITEVAYDENGNKTITFANRVLSSNGTYLGILRVKYSSNVLQNIIVDSSQ
                     TSANALVLLLDEVYIRLADSRNSGLIQKSIAPLSPVDYLQAVESKRFLNTSPEEQSTN
                     FVEFENALDNASVTPFFRVDITPNIPGDDTVAVAPMKTRPWVVTYSRPTSLFLADVQT
                     QTRANTALVIGALILISILTTVVARNLTAPIVALTKTANLISQGDLSARAEINTTDEI
                     GQLASAFNSMTNQLSNSLSGLEERVFERTADIQRRNLELETISDVAREISTIRDLDTL
                     LNVSTSLIREKFNFYHVGIFLVDERGEYAILRAASSVAAEELLARKYKLRVGQTGLVG
                     NVTSTGQAYIALDVGRDAVHFENPFLPNTRSEIVLPLRNYNVAIGALDIQADVPTAFD
                     ERDMQTFQILADQLAAAIENAQLAHQVAENLAALTNANRFQTQQTWRTVIEQQRHSAY
                     EYDGLQIKAVPQDLPADIMKQLENGNPIVYQQPTQGRDVKHILMVPLIVFNQVIGVIG
                     LEQEKADHNWTDEDVTIAQAAANRAALTLENARLLEESQRRAIKERAIFEATSRIGSA
                     LSVENILQATAEELERVISGSEIILQFNTEGSSGINDKDEL"
     gene            complement(16623..18377)
                     /locus_tag="PL000_10245"
     CDS             complement(16623..18377)
                     /locus_tag="PL000_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89325.1"
                     /translation="MSISKPPLNTQTASRFERIQQIGQSLIAPAPSIKDIGQFRRAQL
                     LAILTLITSVLFFAAIFSGPRSYGVFIALTLISLASYALSRTTSFRTGTYLFTYAFTA
                     IGFIRIYQGEATSIEASIVSTVHVALVVSSVLLSQRGFLGLVALSTIATFTAPFYADA
                     TIAANSAQAFTSDISRTGGIVFTLGILLYGIQVFRANLDKEKLKELLDTNQELEEVKK
                     NLEQRISSRTLELEKANQQVQERAARFQIVSEISQEISANVEQKPKDLLQSIARSISE
                     KLGFYHVGIFLLDKNREFAVLRASNSQGGLRMMDRHHQLKVGGAGIVGYVAQAARPRI
                     ALDTGTDAVFFNNPDLPKTRSEIALPLKYGSRLIGVLDVQSTQQFAFNDEDANLLSTL
                     ANQISIVINSIIAAENGELGYSQGYFQAEKLARKKDTQAGFSYLPDGSIVTARPLNDL
                     ATEQALASGETVVLSQSSRKVPPTLAIPVKFRDQVIGVIHIQATEKNRKWTEDEVALV
                     QSISDRAALALENARLFEETVRRADQEETIAKVTTQIGASTDFNRILQTTIQELGQVL
                     GVSRSFIQMGPLKNEEEQ"
     gene            complement(18382..20745)
                     /locus_tag="PL000_10250"
     CDS             complement(18382..20745)
                     /locus_tag="PL000_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89326.1"
                     /translation="MRTRSLPTSTKSLNKPPRRGLFGRYVSLQVKLPVVVITLLLLAL
                     FASAYLSIQAAKTALIDTLKNELILQADSKAELIRSNLIWTRAAAIDLAASAESVNYD
                     EETIQKAIRNTLLRNEQVFGSTIAYEPFQFHSNSYYWAPYYSRAANGELQFTQLGNPE
                     YNYFGWEWYTLPKEKNTPVLSPPYFDAGGGEIWMVTWSAPFYNEAGEFKGVATADIAF
                     SQTQDIVSKIEVGKNGYAFLLSPEGVILGIGSNGGQYQTMVDSMIAAAQSSSQASGWA
                     SLVTSMIQGSTGFSEAVDPQGRSMFIAYSPIGLDTGWSLGLAFPQAELFQRAVQLQNT
                     LGVYTLIIAVIFGVFLFVYTLSITNPLRRLAWYVGHFSPEKINQNLEPVQIYTRDELE
                     DLSTTFQQITTNLFNTFTNFEERIAERTRETERRSILLKAVADVGKAITSFRDLSELL
                     QQTTYLINENFGYYHIGIFLLDEHKEYAVLSASNSEGGRRMLEKGHQLKVGETGIVGY
                     VTQNAKARIALDVGKDAVYFDNPDLPDTRSEMALPLIIGGQILGALDVQSTEAQAFSD
                     EDSATLQILAEQLAVAIQNANLFNETQKALEASRAAYSELSREAWTKILRNQPRIGFI
                     ATPPTTTRTYSDTVEPSIAKAFDTGDIIIGSDNLTISIPVKVRGQTIGAMRLKKAEIA
                     EAWTQEEVNLAISLSDQVSGALESARLYRDSQQTGARESLVSDISSRISAVSSTDAIL
                     RETVQELGQVLGDASITFQLLNQPDGQKQTEVELRSGLSGQAGKAKE"
     gene            complement(20774..22435)
                     /locus_tag="PL000_10255"
     CDS             complement(20774..22435)
                     /locus_tag="PL000_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89327.1"
                     /translation="MNSKTPITTSSAFDYIGWREQFIITVLRIACVLGAALIATSFPT
                     ASATDRVLFPSLYLILLAITVLKTPYSVRAASLLFMVYIIGTNSLLSWGPWLDGSIFF
                     IAFTILAALLFDNRIDLIALILSALTFVTLGVLQQLGIYEPSAKGVPPTIATDWAAYT
                     VDFSIVSAIILVATNQFKKALTQVIFEMQSAFNSLTTEREKLEDRVRERTEELEARAI
                     QLRAATSVTRTIAGIQDISELLSETTRLITDEFGYYHSGLYLLDERKRTAFLQASSSA
                     TGKSLIGQGFRIEPNKLNMTNLVIEQRRPQIAMDVGDANFIRDPNFPLTRSRIMLPLT
                     VRGQVIGILDIHSDQPRTFDGQDAEVLQTLADLVAISIDNVRLIDETKSLVYELESNT
                     TLQTRETWSKFTSRHKPAYQYTPAGVRPLFSKIKTEKADGLHVPMILHGQNIGNIVLK
                     RKGISSTWSERERILVEKVAAQVALALENSRLVDETQKNAMRNQVIANISSRVRETLD
                     VESVVRTATTELRRVFDLKEAEISIGAPQPEPTQLRKNTSSLRLK"
     gene            complement(22428..24023)
                     /locus_tag="PL000_10260"
     CDS             complement(22428..24023)
                     /locus_tag="PL000_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89328.1"
                     /translation="MMESYPSTTNASNSPEEATRLLYKSWREQFTMPLLIGSLVLGIL
                     ALIPALSGAGNTFIRVLFISVYVLTGIVTVIRFSYSIRMSVFLTSIFALALAELFTHG
                     ILGDSLFFFLGLIVFATLMVSPKTGIAAIIVNILTFVAVGISMQSGRLVPINPNAPPS
                     NISDWFSAGAAIILFGVVIVLGFQRLEVAIFETQKQIQSTLSALKHERDNLETSVSDR
                     TQLLQKVNRVGRAITAILDPNELLSHAVYLISDELECYYTAIFIVDATNQWAELRAAT
                     GDAGRVLIENKHHLNVNGKSIVSTVLRTKQARVALDTGADPVRSDNPLLPYTRSQIAL
                     PLTIGDRVIGALELHSTKENAFMQQDVDAYQNMANQITIAIENSRLFTEAQQSLAEMS
                     ATQQQYLKGAWLSLSSEQKLEYAVGDSESSDKQEIEVPLTLRDQVIGQISLSSASDWT
                     SEQKNLIESVAVQAALALENARLVEESQSVATRERLINEITSKVWASTTIDSILQTTV
                     RELGRALEAAEVDIEVTMDDDNE"
     gene            complement(24023..27901)
                     /locus_tag="PL000_10265"
     CDS             complement(24023..27901)
                     /locus_tag="PL000_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89329.1"
                     /translation="MTATQTTPGLDSKQKRYKGSLTGTLVRTLLIFTFIPLALMAGTA
                     YFRTRTLLREQANNQAQNLFVSQLNIINREVGDKEVHFQDLLASSDFQIMLELALHSD
                     PKTDEFQVLRDGILQEFSAMNRHENMPAFDQFLLLDTNGNIKIATEEKWQGETLDPAI
                     IGQDFDTQHTLALYGLSPLYEEELLLVTTLRYETKQGAVLGFVVGITEKKSIQNSMQS
                     LSGLPMSSAYLVLSNQQFFQSQPEANRFTLLTSPLEAQSELETALTESRLDGGVSPRS
                     IETTTAENKIKVFQVQWVPKLQVWMILETDTTEAYKSLTSLAPFTAILTTITLLAIGL
                     ALAITINRVIKPLQSLSQIAHSFADGDLSQRAEIKSDDEVGLLASSFNHMAEELGSLY
                     RSLEQTVDERSRQIRTAAEVAQSVTKLSNLDEMLDKTVELLVREFGFYQASVFLMDPG
                     GKALEFKTGFGSATSALIKRKYRLEVNSESIVGWVSTNNQPRIASDVSEDPIHLKNEL
                     LPETRSEACLPISIGSLVLGVLDVQSIQVGVFDTETVAMLQTLASQIATAIQTTGLTE
                     TSQINFEELAQLYRSSRLIAEANTENEILEIGRDAFADAPYPVIILRVVNNKLEMFSQ
                     TDGKSGNSVKKISLPSASEASVVDIHNYLHNGQVIITSEDKNIPAALDSIMQTLRFSS
                     AAVFPLRRGGLLHTVIIMGGQQLFSNAIVQPYANLADLMSVTMEKLDAIQQTEKHLRE
                     VEALASISEVVATSSDMQSFFMNLHAKIRQVIGDYAFIVALYNERDNTVSIPFAYEDD
                     KITSIESFPLGEGLTSILIRTRQPLMIVENAERRMIELGAKFSGKPGRSWLGAPMLVQ
                     NQPVGALIIQDTEKEHAFDEEDLKFFNTIAGQVAGVIHNAHLLDESQNKALQLETAAE
                     IARDISGSLNLDELLIKAVNFIRERFDFYHASVFLHDLPGEFAVIREATGEAGAQMKR
                     SGYKIGIGSKSIVGYVSSRGERLVVGDTTKDATYYANPLFPDTRSEAAVPLKVGERIL
                     GVVDVQSTRPYAFTEDKLRSLQILADQIAIAVVNTELFAETQEHLSQHRLLHHITTTA
                     ASGTTLEEALENAVNGLQVTLGGDRVTIMLANREKNLLEVKASMGYAEDIAKVQVPIG
                     SGITGWAAAHKRPLRIKDVSDDPRYIRISSNTRSELAIPLVYRNELLGVLNVESEQTD
                     AYTENDEEMLGTLGGSLAAIIANARLLEQIRIQAERERLIYEVTSKIRRSTDIQSILN
                     TTASELARLTGAQYTKIRIQPGNDPTQEGN"
     gene            complement(27909..28361)
                     /locus_tag="PL000_10270"
     CDS             complement(27909..28361)
                     /locus_tag="PL000_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89330.1"
                     /translation="MSDQIPAISDTQPIRKPNIPKDAAVLVVEDNVSNFVLIARMLGY
                     LGIHCEWKTSGYEVVEYADTLPRLDLILMDIRLPYEDGYGALKKIRASERFKAVPIIA
                     VTAEASMEQMAKAKDSGFDGFLGKPLDPDRFPDQIRRILSGEPVWEYS"
     gene            complement(28354..28785)
                     /locus_tag="PL000_10275"
     CDS             complement(28354..28785)
                     /locus_tag="PL000_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89331.1"
                     /translation="MTYSKIQVGGSRMIPETKKGTILYVEDNPDNRSLIRRVLESEDY
                     SVIEAVNASQALENLEKGNIDLVLMDINMPDMDGYTLTAKIKSVQKFTKIPIVAVTAN
                     VMRGDREKSLEAGCDGYIQKPIDIDTLTQQIERYITRSSNV"
BASE COUNT         7102 a         6421 c         7367 g         8012 t
ORIGIN      
        1 tcgaaatcct catcggtaaa cgcggcggtc attgtgcttt gaatatcaag cacgccaatg
       61 attttgttac tggccttgag cggcagtgcc atttcagagt gcgtgtctgg caggtctggg
      121 ttgttgaaga aattcgcatc ttcaccgaca tcaagggcaa tacgcgggtt ccctgtttgt
      181 gccacataac ccacaatacc ttgttcaccg attcttaatt gatgactgcg ttgcagcatc
      241 ttttttccac catcactatt tgctgcgctt agaacagcca tatgattggc ggggtcattg
      301 aggaaaattc ccacgtgata aaaaccgaat tgctcactga tcaccctgga aatttgagga
      361 agcaaatcct gcaagttgct tttggcgctg attgcgcggg ccaccttggt aatggcctca
      421 aactgttttg cgcgtgtgtc agactgttgt ttttcttttg caagttctgc ggtacggtct
      481 gcgactcggg cttcgagact ggataccgtc tctcggagtt ggtcggtcat tgagttgaaa
      541 atggatgcca atgcaccgat ttcgtcattg gtttgaagat taacgcgagc ggataatttc
      601 cctgcagcaa catcttgtgc agccagtgcc agattgccca gtggagaagt aattgaattg
      661 ctcagggtgc tcataataaa aatagaggcg atgatcgtta gcaataacaa agataacgca
      721 ttgtaggttg attcgaagat cggttttgtg atcgagtcta tggagacaaa tgagccaata
      781 taccagcgtg gttctgtttc gccagggatc gtgatggggg tatagattac aagatatcca
      841 tttgactcca tacttccaga gtttccattg ttaagattgt ttacgaggtc tggaaagtca
      901 tttttgatgg ttatattgtt gtttagatcg ttgccccacc tcttggattc atcaggatgg
      961 gcaatgtagt aaccgtttgt gtccaccaaa aaagtatcat cacctgatgc ggagagaatc
     1021 ccgaggaatt tctcggcata gatatttgcc atgatcacgc ctttggtttg cgtgttaatg
     1081 acgagaggta caccaaattg aatgatcggc tcatgtggtg tgacgatcgt tccatccagt
     1141 tttctgaggt tgattggaga ctggaataaa cgaccagctg gcagggtgat tgtctgcgcg
     1201 aaatagctta attcccttgc aggatcaagt tcacctttct gccgtagttc cgcctgcggc
     1261 acaatggatg aattaccatc cagagcggta tctattcgaa ttacttcgtt cccgtcgttg
     1321 ttcaggaagc gtatttcttg ataaatcccg cggctttctg caaaacggcg gaattcacgc
     1381 tccatgatgg ttctcgctct gacaacaatg ttgggagcag caatgtcatc gatcgtcttt
     1441 acataacttt gtacaacttc tgcttctccg aggaagacta catcctgtct ggaagcttcg
     1501 aggaaatcga taatgaccga tcgttttaca ccaagttcgt tttccaggtt gtttgcggtt
     1561 tgtgcccgct gagttctgta gctttggacg gcattgtaac cacctgtgac cagcaatgat
     1621 gtgacaagaa ttgtaataaa gacaagcgtc agttttgttt ggatgttaaa tgttctaaat
     1681 tgacgccaga tcaataccat aaaaatggca gtttcaatta tcgcaaggat ggctattgta
     1741 accaccaaaa cgcttgggat ttgaattcta acgggaaacg ggatggatcc aagcaggatg
     1801 atgccgatcc cagtgatatt gctgactacg atagcccctc gggcaaccct ggtctcaagg
     1861 gtctgactca aaattagtgc tgctgccaac gtgctaaaaa gaccagccag ggtggctatg
     1921 ccttcgaata gaatcggcaa tagtatgatt gttaagtgga ctgaaccagt tactagaaaa
     1981 atcccaaaca aggtgttttt cttttgaagc tgttgcaagc cagcatatgt caaaaggata
     2041 tataaaatat tgccaccata caaagtaaaa agctgccaac tgttaaggct gatggcaatg
     2101 taaagaatgg agaatgaaat gatcgtgctg gtgacaagga atgctcttgc aactgtcagc
     2161 gcaatacgct cacgttgctc ttgttccctg ttttggaata tgggggtttc tgaataagca
     2221 gcagtcatta ggggattact ccatattctc gttcttttgt tcgaattcaa tgagtacttc
     2281 ggaccccgga atggcgtggc ctaactcttc tactgccgtt tgaagtacac tctttaagtt
     2341 gatggatgcg ccgatctttc cagtaacttc actgatggtt tgttccttga ttacctgtct
     2401 ttgcgattcc tgaatcaggc gggcattttc aagggccagc gaaagacgct ctgaaattgc
     2461 ttcggcaaga ttgacttcat ttctggacca ggtgcggttt gctgaaggtg ctttgatgtt
     2521 cattgtgcca ataatctgtc cacgtagttt gatcgggaca acgatcgttg cttctcgctt
     2581 gtctgtttct gaattaagca cgaggacgtt tccgcggttg attgcttcgc ggatttcttc
     2641 ggtctcaacg ggttttgata tttttttgcc acccattaag ccctggtggt acccaatggt
     2701 ctcttcttcc cgactgaata ctctccaact gctttgcagg ttttgacgat actgcgcctg
     2761 agcctcagca agagcttgtt gattttgtgt gaacaagcgg gcgttttcga gggcaattgc
     2821 gatttggtcg gcaagaatac taagtgtact ggcatcgtat tcagtaaacg cgcctggttt
     2881 ttcactttgc acatcaagta cgcctacaat acgattgcgg accttaagag gtagtgccat
     2941 ttcagagcgc gtgttgggga ggtcgggatt gttgaagaaa accgcatcat gcccaacgtc
     3001 aagcgagatg cggggcattc cgaattttgc aacataacca acgattccac tttcgccgac
     3061 ttcgagtttg tgtccgcgtt caagcatttt ttggccgccc tcactgctgg ctgcctgcaa
     3121 aacagcaaac tggtttgtct catttagcag gaagatacct gtgtgataaa agccgaaacg
     3181 ttcacttacg agcctagaga tcaatggaag aagtttttcg agggcttgtt cgcctgtgat
     3241 aacttttgag atttcgccaa tggcttgtag ttcgctggcg cgtttttcgc tttgacgacg
     3301 ggcgatttca aggtcggtgg ttctttctac aacacgaatt tcaaggtttt tgaaggcttg
     3361 gtttagctca tctgtcatgc ggttgaatga cttggcaagc aagccgattt catcttctgt
     3421 cactgcctca gcgcgtgccg aacgattgcc gactgcgatt tgttcggcgg tttttgcaag
     3481 agtcaggagc ggtgaggtga tcacttggga ggcaaggaac cccgcgccaa tgctcaggat
     3541 gaccatgatc agtgaaatca ggatggtcgt cctgttttgg tcttctactg gtgccagata
     3601 attggatttg gattgcctta cgattgcaac ccacggttgg gtttccagaa aaacaccagt
     3661 gtttgtggtt gacccgagtg attttgatgt ggcatcgaaa aatgattgtt gttgtagatt
     3721 gtcaatgcca tccaccattg caacatctgt ttccgttaaa agactttcta ttgagatgga
     3781 aggcagccgt ccttcagatt gcaaaactgc gagatcaggc gccgaaaaat ctttataggt
     3841 cttgaaaagt tcgtcacggt cacctgtgta ggccacacgc agatatgtat tagtgtctac
     3901 aagcgacagg gttgttcctg cattaagagg atcgactgag cgcacaattg cctgaattat
     3961 tgtagcgtga tattcaactc gcaggatgcc aatggtgttt ccgttttgtg atttgatggg
     4021 tgcggaaaaa tatatgtggt ttcgattgtt ctggaataga atgttggacg caaatggaag
     4081 gcctgtttgc attggacgaa cgaaatatat caagctattc tctgtttgac ccttgtagtc
     4141 ttcaaaggtg tcaataagat ttgttccatc ggtgtctaaa atggcgatcg aatgtatgaa
     4201 taccgggtcc ctgcgtgtta acgccagaag cgccagacgc gcatttaact cttttacgct
     4261 gcccgaacgg gcggcggctg gcaggcgaag atattcaacg aaagccagct gttttgcgtc
     4321 agcgtgaaca ctgtctattt gattcgtgat gaagttgtcc acgctttcag cttgaatctt
     4381 tgcaatattg gtcagttgta gtttgctttg gttttcgagg attcgggtgg atgacaggct
     4441 gtaaaaatac cccagagtaa caagaggggt gatcgtgatt agtgtaaatg ccgtaataat
     4501 cttggtccgt agtgagtaag tgttaaagct tctaagaata aaggcgatat aagcaagtga
     4561 tgtcaccaaa gcgacgatat tgatgtaagt ggggtttccc ggcagcccta aatccgggag
     4621 atagagatcg atcagaatga tggcggcagc cacaatgaat gcgattatga ttgatcgtgt
     4681 cgtccacttg gctgataggg tagtggagga aatgcttgcc accacaacga ggaccaggat
     4741 gcttagtgca agaccgtgtc cttgtgcaac gatcggtatg gcaaaggaga ccagcaaaat
     4801 cgcgaaaatt aagaacccaa tgccgattga agtacgcccg cgataggcct gtatggatga
     4861 gaagaatgca gccagagaaa tgaagccagt aattgcgccg ccccagaggc cgttcatgcc
     4921 attttcttgg tagccgacaa tgacagcaaa cacagaaaca acaagaaaaa ggatgccggt
     4981 gatcaggctg attcctagag cgtttctttt gagacgggct tcttcttgtt ggtcgcttgt
     5041 gttttttaca ttcatgagtt actcacttgt tgatttgtac agttaaggca ggtatcttga
     5101 gtctagtaat tctaacgggt ctggcatttg agtgattgtc ccaattgcga ttaggaaatc
     5161 ggatgtgatc ttggtaaccg tgtagatgga gttttcactt tcaagcctaa agatggcttt
     5221 attatcttcc aatgtaagaa tttttagatt gtcatctggt tgcacttctt ccacacttac
     5281 acccagatat ttggcggcaa tggcgcgggt ctcttctggg ttttgcaagc ggtagtcaac
     5341 agcttcaaac catgctttta gaaaggcgcg cacatcatct ggtctttggt ctgtcaccga
     5401 tttttggaag acgatcagat cgggaaagag atgcaattgt tcttgtgggt ataggacttt
     5461 gtatcctttt gataacgctt ctgataaata aggttcccaa atataaacag cctgtacctg
     5521 gttgttttca agagctgcca aaccatcttc cgggtaaatg gaaacgacct taacatcggc
     5581 aatatccata ttcgcactgc gaagcatttc tgtgatcatc aactcgtatt gtgagccaat
     5641 ttgggttcca acggtcaggc ctcgtagatt ttcaatcgaa aggatttcag gccttgcgac
     5701 catggcatca tctccgccat tgtcgtgaac tgcgactact ttcattgggg cgatacggga
     5761 aatattgatg gtatctccaa ctgcgatcag acctgcatcc agctgacctg cggcgagatc
     5821 aggataggta tctgaaaaga actcgtagta cacaggctcc acctgtacac catatttttc
     5881 aaaaaagcct ttttcctgtg cgaccagcag tgtgtaatca ccccaccaat tcgtaaactc
     5941 aacgcggagt ggcggcagtg gggcagacac aggctgtgaa attgccgtgc aggcagaaaa
     6001 aataacggta agaagcagca cgggcaggaa cttggaggtg ggggttcgca ttattgattt
     6061 tccataatac tagctggaca tgcctaattc cattgatact ggctcgatct gcaccagaac
     6121 atctgacccg ccaagtgcct tactgagttc ctgagcggtg atttgcaaaa tggcgtcgaa
     6181 gtgagtggag gagcttatct ttgaggaaat atctgctgtc acacgttcga tatctgcgcg
     6241 gtgttgggtt gtccttaaga gtgtggctgt ttcaattgca agtgaaagac gctctgaaac
     6301 tgcttcggca atatcagcgt catcttttgt taatacgtaa tcattttttg aacttagatt
     6361 catgattcct ataatttttc cacgcatacg gatgggaatg gcaagttgtg aaactttccc
     6421 ttccggattg tctaagatcg gcttttcctg tgagatcgct tcttgaatat gatcaccgtt
     6481 cagtggaaca tccaatggtt tgattgatga atttaccaat tgatacccca agccaagaga
     6541 ctcaggttgc atgatcttcc atgattccag aacggcatca ccaaatctgc tttgcgactc
     6601 tgcaaaggct tttcgcgatt cttccaggac ttgtgtgtta ttgatcgcaa tcgaaatctg
     6661 atcagcaagt gtggttagtg cctccacatc gttttggccg aaggcattgg cttcagtact
     6721 ttgtacgtcc aaagccccaa tgatctggcc tgcatagcga agtggtagtg caatttcgga
     6781 gcgggtgttg ggcaggtcag gattgtcaaa gaaaacagca tcagatccta cgtccagggc
     6841 gatgcgaggc tgacctgttg cggtgacgaa acccacaata ccggtctgac cgacctgaag
     6901 cttgtgtccc cgctcaagca tttttctccc cccttcactg tttgcagcac ggaggatggc
     6961 atattcgcgt tcgtcatcaa gcaggaagat gccggtatga taaaaaccga actgctcgct
     7021 gatcacttgg gcaatacgag gaatgagcag ctccaggtct tggatgcttg aaactacacg
     7081 gacgacttgc gaaatggctt cgaattgacg ggcacggcgt tcattgttcc gattcgcgaa
     7141 ctccaattct gatgtgcgct gattaacgcg ttcttcaagc atggaacgca gttcgttcag
     7201 ctcagcgttt tctgttttct ggatctgttc actttcacgg gcacgtttaa tgctatcgtt
     7261 taggcgggtg atcaatagct gtacaatggc agcagtaccc aatagcaaaa caggaagaat
     7321 aatcgcatcg tcaacgccag cagggataat agggatttga ttttgtaaat ttgcgacagc
     7381 aacgattgtt acagcgatga tggcgacagg tgtggttaga taaatagagc gacgtccaag
     7441 caggatggca gaaattacca gaatgatagg cattccaacc atggctgcgt tctttaaccc
     7501 attggatgcc agagagatga tggttaccgc agcaattaat gcaaatggaa ccaccacttt
     7561 tgccatgccc agttttccgc gtgtaaccag gaatagtgag atgatttcaa gaattaaggt
     7621 ggttaggagc gtgaaaaatg cgcgcgggtt acgagagccc tctcctacaa ttcctgttac
     7681 gagtggaaga agcgcgatat ttacaaccaa tacgaaaatg acaatgttgc gcaccaatct
     7741 gataaaagat gggtcgctgt cttcttcatc tctaaaataa ttaatgatct ttaacaacat
     7801 atcattctcc tgagcgtttt acttttgctc tagctcttca ggggagattt ggatggcgat
     7861 atctgtattg ggaatggtat tcccaagttc ctgtatcgaa gtttgcagga tgctttcgat
     7921 gttgatcaaa ccgccgatct ttgcagtgat ttgtccgatg gcacgctctt tggctgcccg
     7981 tttttgggct tcctgcaaca aacgggcgtt ttcaatggcg aatgccgtac gttcggcagt
     8041 ggcttgggcc atcgcgatct catcgtcggt ccatgtgcgg tttggattgt ctgtatttag
     8101 cttaagagta ccgatctttg ttccgcgcaa gatcaagggg attgtatagg tgttctcatc
     8161 cttggttttg acttgcttga tgtcttttgc atcgaggccg tcaaatacaa agccatttga
     8221 aggctgttct gcgaagaagc ggccccattg caactcgctc atttgcatgg atgcggcttc
     8281 ggcttggtcg agtgcatcac ggctctgttg ataggaacgt gcattttgga tggcgatact
     8341 tacctgatca gcaagtgtgg ataggatgct gacatcttcc tgtgaaaaag catcagtctc
     8401 gcggctttgt acatccaaag cgccaatgac ttgtgtacca atcagcaggg gaagcgcaat
     8461 ttcggaatga gtatctggaa ggtcaggatt gttgaagaaa gcagcatcca ttccaacgtc
     8521 cagcgcgacg cgcggctgtc cggagttggt aacaaatcct acgataccgg ttccgccaac
     8581 cagcaatcta tggttgcggt tgagcattct tttgccgcct tcactatttg cggcagcgag
     8641 aatcgcatat tcgcggtggg catccaccag gaatatgccg acgtggtaaa agccaaattc
     8701 ctgtgagatc gtatccacga tttgagggag cagtgagttg agcgtttgag ttgaactgat
     8761 tgtgcgggct acacgagcaa tggcttcaaa ctgaccggcg cggcgaatga tactttggtt
     8821 tgcctcctca agttcccgag tccgctcgag aatgcttttt tcaagcccaa ggagcgtgga
     8881 attcaagatc tcagccatgc tgttgaatgc cttggcgagt acacctgttt cgtcacctgt
     8941 ttctactaat gcacgcgcgg caaaatcgcc ctgcgatatt ttttctgctg tctgtgtcag
     9001 gcgcaccaat gggcgggtga taagctgccc gatccataaa ctgacaatga ttgcgccaac
     9061 aagcaatacg attagaatga ttgccacacc ctgcagggcc gattgaacaa tatcctcgat
     9121 ctcgctcctt gattgaacga actctgttgt gaattcgttt tcaggtgcta cgatcccaag
     9181 gcggtaattg ggtactgtaa cgggtgagaa aatgaagaaa gaattaccgt ctccattttt
     9241 gatggttgca atctcgctgt caccattgat catttgagcg gttgcttcct gtacatcgaa
     9301 aggacccttg gacaggacgg aaagctcatg aacggaatta gttgtgacct cttcgggtga
     9361 aatattgtaa aacagatatc cttctggcgg catcgacaga atatgaccgg ctttatcaac
     9421 aaggaatgca taccctgtat ctccaacctt tacattggca atggcacgtt caatttgggc
     9481 tagttgaaca tccaaaccta tgacgcctaa gaatgtatcg ttttgataca cggggatgga
     9541 aagtgtggtg atcaaaccat tgcctgcagg gtcttcgtag gtatctgtcc aacgcggctc
     9601 acgtgaggga ttgttttccg gggaggcaat tgtgtaaaag atctctttgg tgggatcaaa
     9661 atctgcagga acgttttccg caagcctgat gttggggtag taggtggtgg cacccgctgt
     9721 gttaatgaaa tacaaggcaa ttacactgga gtgtgtttgc aggaaattgg gggcataaaa
     9781 atcaagatat gctgttgtat ttaaatcatt gtaaagagat tcatccacta caactgtaga
     9841 agggataaat atcgaggcta catctgctgg ggagttgccg tattgtcctt ctgttaattg
     9901 gatgacttta ttgtccgcat cccaatatgt gctgtcaata aagatatctt tttgttgttc
     9961 gaggctggcg cggtattttg ccagaatccc caggtcgttt tgaagtttgt cgaagaagcc
    10021 attggtttga gcggctctgt cttccacagt atgaagcagc tgattttgaa tgttttctct
    10081 ggatgccgtt tcgtacctgg ttgtgagtgt gttgatgatg tttccagtac ggctggcggc
    10141 aaaccaaacc agggtgacca caatgacgcc gcctgttaag agaatactaa tggtgatttt
    10201 gatgcgcaaa ctaaaacggt taaagtggcg gactgtgaag gcgccaaaga ataatgcgaa
    10261 accaactacg atgaaaacag tggctctctc cagctctggc acgcgaactc ttgcctcgcc
    10321 aaggaacaaa taatcaagca gaaggatcag cgcaccagat gagagtccga cgatcaagcc
    10381 tgatgctgca tatcttgtag gcattgccag gcccgtgatg gacactgcca aaataaacat
    10441 gcttatggca aggataacac caaggccttt gatgaaaaat ggtattgcaa caaatgtgat
    10501 ggtgaaactg gtgattatga tcatcatcgc aagatttgat ctgccgcggc gtatcaggaa
    10561 aagagcggcg actgcaccaa aactcgcaat gaacatgatg atggatatcg ttaatatttg
    10621 cgcggaaccc ttgagcaaag cataataaac aaacaagcca acgcttggaa tggctgtgat
    10681 caacagcatg atgctgatgg tatatgcatt gcgtacgaga acgctatcta cctgctgtga
    10741 attggatgcg cgttccatgt ttgtatctct attcatcatt gtcgcttcca atctcaaccg
    10801 aaatctgggc gccgctgatc tgactgccca actctcgaac agctgttttt aagatcattt
    10861 cgatctctgt gccagcactg atctttgagg aaatttggcc aatggtttgt tcacgtaaag
    10921 cacgcctctg tgattcctca agtaatgttg agttttcaag tgcaagcacc acacgttccg
    10981 cagtagcacg gacaatatct gcttcatctt cacccatgtc gatatctgcc ggggttttga
    11041 catggatgac accaactgtt tcaccgcgca ggatcaaagg aacggccagc acactgccat
    11101 ctgcgcggtt ttgatcttcc ggtgcaagga tgaccgtttt tccactttca aaggcagctt
    11161 gtaactcttg tgaatcaaca ggtttttcga gcagcatggg tatggtgcct gaaaaacggt
    11221 agcccaattt gttctcaaca cggacatttg tctgccatgc ttgtttaatc actgtgccgt
    11281 ataagatctg gctttcggcg agcgcggcct gtgtctcttc gtatgcacgt gcattttgaa
    11341 tggcaattgc agtctggtca gcgaggatgg atagaatttc aatcgcaact tcatcaaacg
    11401 cataggtttt gtcactatgc atatccaata cgccgagcac tttgttttcg attttcatgg
    11461 gaagaaccaa ttcagattga gttttttgga attcctgaga atcggaaaca gatttaattt
    11521 gattgtgatc ttgagatact ctcgaatacc cagtttgagc tacagatggc acaaagcctg
    11581 cctgcccaat tttatattgc agccctccag ccattaattg tttaccgatt tcactgttgg
    11641 cagccactaa tgaagtgtat tcctgtgtgt cgtcgttcag gaaaattcca acatgatagt
    11701 agtcaaaacg ggcactgatc acttcggcga tggcaggaag caatttgttg atgtcgcgga
    11761 tgctggcgac agtgcgtgca agttgagccg cggcctcaaa gttcgccgca cggcgttcat
    11821 tgaaggcgtt gacttcatta agctctcttg tacggctttc gatgcgattc tcaaggtttg
    11881 cttgtagaga aaggagctct ttattcgctt cgacctgtag tgctgcattg tttcgagctt
    11941 cttctgcgct ttcattcagg cgggttatca gtaattgaag cagaaaagaa attgcgatca
    12001 tcaaaatgcc agcaatcaag gcgtctgaca cgtcggtctt caatgccatt tctgaattcg
    12061 taataccggt catgtcggca aaagccacaa tctccacaca gagtacggcg atgggggtgg
    12121 agattatcag ggcgcgtttt cgcaacaaca atgcgctcac tacgattact gttgggaagc
    12181 cgagcattga gaggtcgtgc aaaccgttgg cgttgatcgc tgtgtaggtg accgcaacaa
    12241 acagggaaac aggcactaca atttttgcag ggatcggatt tccacgcagt acaaagatca
    12301 gtgaagcaat ttcaagaacc agcgttaccg ataatgcaac gcttgaggct gcgttttgcg
    12361 attctctgaa aaataagcca acaaccagta aaaggatggc gaggttggca atgatcgtga
    12421 aaacgaggat attcctcgtc attcttagga atgacgggtt ggtatcttcg aggtctgcga
    12481 accagttgga tgacataata ttgccgctct ccgattaact gtctgtgtcg ttcttttttt
    12541 gcaactgaat gacaacttca gcgccgggca aggcccgccc gatctctgca actgcggcct
    12601 gcaaaatgtt gtcgcggttg gtgaaagagc ttactttgga tgagatttct ccgatcacgc
    12661 gttccttttc agctgtcttt cggctttctg ccagcaagcg ggcgttgtcg attgacaatg
    12721 ccgcacgctc tacaatggcg ttgatgatgt ccagttcatc ggtgccgatg gcattttcat
    12781 cctctgtttt gatgtttaaa agaccaacaa cttcacctcg taatttcatg ggaatcgtta
    12841 acgaggtatt ttggtcatct tcacttattt tgtagatcgt tccggagcgc acaacttctt
    12901 ttgcaccagg tacttccatg gggtcaaaaa gcagacttga tttcaagccc tgtttgcgga
    12961 ttccagaaag tttttgagaa cggacgaatt tactccagcc tgcttgaata ttgcggcgat
    13021 ataacaattg cgactcaagc agaacggttt gtgtttcttc ataggaacgg gcattggcaa
    13081 tggctatgga tacctgatct gccaatgtgg ttaatatctg gatgtcatcc tgtgtaaagg
    13141 catttgattc cgtgctttgc acatcgagaa cgccaattag ttcctggccg cttcggaaaa
    13201 gagggagagc gatctcagag cgggtttctg gaaggtcagg gttgttgaag aaagtggcat
    13261 ccagtccggt gtccagcgcg atgcggggtt ttcccgttcc tgccacgtta cccacgatac
    13321 cggtctgacc aatccgcagt ttgtgatcgc gattgatcat ccgctgcccg ccttcgctgt
    13381 ttgtggcaga taacacggca tattccttgt ttgagtccag gagaaagatg cccacgtgat
    13441 aaaacccgaa ttgctggctg atgacctggg caatttgagg aagcaggtct tgcaggtttt
    13501 gagtggaaat aatggctcgg gttacctgtg cgatggtttg aaacatttgt gcgcggtgtt
    13561 cgctaattcg gttaatgcgg cccaaatcct gagtgcgttg cttaacacga tcttcaagta
    13621 aatttcgttc ttcttcgatc ttctgaaatg actcccggac acgattgcgg gaggattcaa
    13681 actcgacctg aataagcctg atgccgttga caattacaac ctcaagcaat aacgccgctg
    13741 tgatggcact tatccaggta tcaaaatttc ctggagttac atttttactt gaaatggtga
    13801 tggctcctgt aacaatcagc cagcctgcga ttatgaacga agccatggat aggcctgtca
    13861 tgatccaacc agcccgccat gataataaaa gggtggccat ggtaacggcg gcaagcatga
    13921 aaaggcgggc atctccacgg atccccgttt ccaaaagacc ggagatgcca agcagaaaca
    13981 ataaacctac aagtgtcact gctttaatcg tgtatggtgc aggcaggatg gtgatcagta
    14041 gcagtgcgat atatgcgccg atataaatac cgataagaat gggatcggcc gttccgagga
    14101 cggctggaat gatcgccaac aacccaaata cgcaggcgcc aattaatgtg gcttgcagaa
    14161 atgaggcttg ccatcttgtg taggaaaaat caaggttttc agaaggttga ttcatgattc
    14221 aataaatccg tggcgtttgc cgtgttttta cctaaagttc gtccttatca tttatgccag
    14281 aagagccttc ggtattgaat tgcaaaatga tctccgatcc gctgatgacg cgttccagtt
    14341 cctcggcggt ggcttgaagg atgttttcca cgcttagagc cgaaccaatg cgggaggtcg
    14401 cctcaaaaat ggcgcgttcc ttgatcgcgc gtcgttgact ttcttcaagc aaccgtgcat
    14461 tttccagggt cagtgctgca cggttggctg ctgcctgggc gatggtcaca tcttcatcgg
    14521 tccagttatg atccgctttt tcctgttcga ggccgatcac accgatgact tggttgaaca
    14581 ctattaatgg aaccatgaga atgtgtttga catccctgcc ttgggtgggt tgttgatata
    14641 cgatcgggtt cccgttttca agttgtttca tgatatccgc aggaagatcc tgaggcacag
    14701 ccttgatttg caagccatcg tattcgtatg ctgaatgcct ttgctgttcg atgaccgtac
    14761 gccaggtttg ctgtgtttgg aaacggtttg cgttggttag cgcagccaga ttttcagcta
    14821 cctggtgtgc caattgcgcg ttttcaattg ctgcggccaa ctggtcggca aggatctgga
    14881 aagtttgcat gtcacgttca tcaaacgcgg ttggaacatc tgcctgaata tccagtgcgc
    14941 cgattgcgac attgtaattg cgaagaggca acacgatttc ggaacgggta ttcggcagga
    15001 atggattttc aaaatgcaca gcatctcgac cgacatcgag ggcgatatat gcctgacccg
    15061 tacttgtaac gttgccaaca agaccggtct gacctacccg cagcttgtat tttcttgcaa
    15121 gaagctcttc agcagcgacg ctgcttgctg cccgcagaat ggcatattcg ccgcgctcat
    15181 ccaccaggaa aatgccgaca tgatagaaat tgaatttctc gcggatcagg cttgtggata
    15241 catttaacaa tgtgtccaga tcacggatgg ttgagatttc acgggcaaca tctgaaatgg
    15301 tttccaattc gaggttacgc ctttgaatat cggctgtacg ttcaaagacg cgttcttcca
    15361 aacccgaaag actgttgctc aactggtttg tcatggagtt gaaggcggag gcgagctggc
    15421 cgatttcatc ggttgtattg atctctgctc gggcgctaag gtcgccttgt gaaatcaagt
    15481 ttgcagtttt ggttagtgca acgatcggcg cagttagatt tctcgccacc actgtggtga
    15541 gaatggaaat caggatcaac gcgccaatga cgagggccgt gttcgcgcgg gtttgtgttt
    15601 gaacatcagc aaggaacagg gatgttggac ggctgtaggt gacgacccag ggacgagtct
    15661 tcatcggcgc cacggctaca gtgtcatcac caggaatgtt gggtgtgata tcaaccctga
    15721 agaatggtgt cactgatgcg ttatccagtg cgttttcgaa ttctacgaag ttcgttgatt
    15781 gctcttcagg ggatgtattc aaaaagcgtt ttgattcgac tgcctgcaaa taatccacag
    15841 gggaaagcgg cgcaatggat ttttggatga ggccagagtt acggctgtct gcaagacgaa
    15901 tatatacctc gtctagcagc aatacgaggg catttgctga tgtttgcgaa ctgtctacaa
    15961 tgatgttttg caaaacattg gaggaatatt tcacccgcaa aattccgaga taggtgccgt
    16021 ttgagctcag cactctgttt gcgaacgtga tggtcttatt gccattttcg tcgtatgcaa
    16081 cttctgtaat gacaggttgt gtgctaaatc gtacctgcgg gaaatacgct tcgtcagatt
    16141 cattcttttt ggcgcgttgc ggcgcggaat ccaataggat atttccagca acatccaaaa
    16201 tggcataaga gataatgttc tgactatctt ttgagcttag tttattgagc aaaccctggg
    16261 cgcggaattg ttctggcctg cctgtgatgc gctgtgaatt tggtagtgac agatattctg
    16321 tgaaaacaga gaattgcgac tcgatcacaa ttgaattcaa ggtggtttga atgaaagtgt
    16381 cgaggctgtt ggcggtttgt tccgcactgg attgcagtga gatttcagcg ctgttcgtca
    16441 acgcttgctg ggtttggagg ttatcgcgta cggcgatgat ggtcagcggg acaagagcaa
    16501 tgaacagcac gaaaagcacc aatcgcgttt gcagcgacag gttgttttct cttcctgtga
    16561 aggttttggt tgtttgtgcc ggtgtgttct gactcatgtc tgcctttatc aatgccagaa
    16621 tgttattgtt cttcttcgtt ctttaacggt cccatttgga tgaaggaccg cgatacacca
    16681 agtacctgcc cgagttcctg aatagtggtt tgcagaatgc ggttgaaatc tgtagaggcg
    16741 ccgatctggg tggtgacttt tgcaatagtt tcttcttgat cggcacgacg gacggtttcc
    16801 tcgaacaggc gggcgttttc caatgcaagt gcggcgcggt cggaaatgga ttgaaccaat
    16861 gcgacctcat cttcagtcca cttgcggttt ttttccgttg cttgaatgtg aatgacgccg
    16921 atcacctgat cgcggaattt gacgggaatg gcaagggttg gaggaacctt cctcgaagac
    16981 tgggacagta ccacggtctc gcctgatgcg agtgcctgtt ctgtggcaag gtcgtttaac
    17041 gggcgggctg tcacaatgct gccatctggc aggtaggaaa atccagcctg agtgtctttt
    17101 ttcctggcca gtttttcagc ctgaaagtag ccttgtgaat acccaagctc tccattttca
    17161 gcggcaatga tgctgttgat cacgatggat atctggttgg caagcgtact taatagattg
    17221 gcatcttcat cgttgaaggc aaattgttgc gtgctttgca catcgaggac accgatcaac
    17281 ctggagccat atttgagcgg gagcgcaatt tctgaacgtg tttttggaag gtcggggttg
    17341 ttgaagaaga cagcatccgt acccgtatcc aacgcaatgc gcggacgtgc cgcctgtgca
    17401 acatagccca caatacccgc accgccaacc ttcaattggt gatgacgatc catcatgcgc
    17461 aacccgccct gactgttcga ggcacgcaac acggcaaact cacgattctt atcgagcagg
    17521 aaaatgccca catgatagaa gccgagtttt tcactgattg accttgcaat ggattggagc
    17581 aggtctttgg gtttttgctc tacatttgct gaaatctctt gtgagatctc agaaacgatt
    17641 tgaaaacggg cagcccgttc ctgcacctgt tgattggcct tttcaagttc gagggttcgg
    17701 ctgctgatcc tttgctcaag attctttttt acttcttcca gttcctggtt ggtgtcgagc
    17761 agttctttga gcttttcctt atccaaattg gcgcggaaga cttgaatgcc atacaacaat
    17821 atacccagtg taaaaacaat accgcctgtt cggctgatat cactggtaaa tgcctgtgct
    17881 gagttcgcgg caatggtcgc atctgcataa aaaggagcgg taaaggttgc aattgtggaa
    17941 agcgcaacaa gcccaagaaa ccctctttgt gaaagcagta cactcgaaac tacaagagct
    18001 acatgtacgg ttgaaacaat agaggcctca attgaagtag cctcgccctg gtaaattcga
    18061 atgaagccga tcgctgtaaa tgcataagtg aacaggtagg ttcctgtcct gaaacttgtt
    18121 gttcggctca gggcatagga tgccagcgaa atcaatgtta gagcaatgaa tacgccataa
    18181 ctgcgtggcc cggagaaaat tgcggcaaag aagagaacac ttgtgatcag ggtaaggatc
    18241 gcaagcagct gggcacggcg aaattgaccg atgtccttga tcgatggggc gggcgcaatc
    18301 aggctttgcc cgatctgttg gatgcgttca aatcgggaag cagtttgagt gtttagcggt
    18361 ggctttgaaa tggacatgac tctactcttt tgcctttcca gcctgacctg acaacccgct
    18421 tcttaactca acctcggtct gcttctgtcc atcgggctga ttcagcaatt ggaaggtgat
    18481 cgaagcgtca cccagcacct gcccaagttc ctgtacggtt tcgcgtagaa tcgcatctgt
    18541 gctggagacg gcactgatgc gtgaagatat atcagaaacc agcgactcac gtgcgccagt
    18601 ctgttgcgaa tcacggtaga ggcgggcgct ttcaagcgca ccacttacct ggtctgataa
    18661 tgaaatggcg aggttgactt cttcttgagt ccatgcttcg gcaatttcgg cttttttgag
    18721 gcgcatggca ccaatggttt gtccgcgtac ttttacagga atgctgatgg tcaggttgtc
    18781 actgccaata ataatatcgc ctgtatcaaa ggcctttgca atactgggtt ccacggtgtc
    18841 ggaatacgtg cgcgtggtgg tggggggggt ggcaataaag ccaatgcggg gctggttgcg
    18901 aagaattttg gtccatgctt cgcggcttaa ttcactgtaa gccgcccttg aagcttcgag
    18961 ggccttttgg gtctcgttga acaggttcgc attttgaatg gctacagcca attgttcagc
    19021 caaaatttgc agtgtggcac tgtcttcatc tgaaaatgcc tgagcctccg tgctttgcac
    19081 gtccaacgcg ccaaggatct gtccaccgat aatgaggggc agggccattt cagagcgggt
    19141 gtctggaagg tcgggattgt caaagtaaac cgcatccttt ccaacgtcca gagcgatacg
    19201 ggcctttgca ttttgtgtca cgtatccaac gatgcctgtt tcgccgactt tgagttggtg
    19261 tcctttttcc agcatgcggc gtccgccttc actgttggat gcgctcaata cggcgtattc
    19321 cttgtgttca tcaagaagaa agatgccgat gtgataatag ccgaagtttt cgttaatgag
    19381 atatgtggtt tgctgcagaa gttcggaaag gtcacgaaaa gacgtgatgg ctttgcctac
    19441 atctgccaca gctttcaaca agatgctgcg gcgttctgtt tctcttgtgc gttcggcaat
    19501 gcgttcttca aagttggtga atgtgttgaa aaggtttgtg gtgatttgct gaaatgtggt
    19561 ggatagatct tccaattcat cacgtgtgta aatttggaca ggttccaggt tttggttgat
    19621 cttttcaggt gaaaaatggc cgacatacca ggccaaacgc cgaagcgggt tggttatgga
    19681 cagggtatat acaaataaga acacgccaaa aataacggcg atgatcaggg tgtaaacccc
    19741 aagcgtattt tgaagctgta cggctctttg gaataattcg gcctgtggga atgccagccc
    19801 aagagaccag cccgtatcca atccaatggg agagtaggca atgaacatcg aacggccctg
    19861 tggatccaca gcttcggaaa accctgtact gccctggatc atggaggtaa ccaggcttgc
    19921 ccagcccgat gcctgtgagg aagattgggc agccgcgatc atggaatcga ccatggtctg
    19981 atattgtccg ccatttgaac cgatgccaag aatcacacct tcagggctta gcaggaaggc
    20041 atacccattt ttgcctactt cgatcttgct tacgatgtcc tgtgtctgtg aaaaggcaat
    20101 gtcggcagtt gccactcctt tgaattcgcc agcttcatta tagaatggcg cactccatgt
    20161 caccatccat atctcaccac cgcctgcatc aaaatacggc ggtgacagga cgggtgtatt
    20221 cttttctttt ggaagtgtgt accattccca gccaaaatag ttgtattcgg gattgccgag
    20281 ttgggtgaat tgcaactctc catttgcggc acggctgtaa tatggcgccc agtaataaga
    20341 atttgaatgg aattgaaaag gttcatacgc aatggtggaa ccaaaaacct gttcgttgcg
    20401 tagaagcgtg ttgcgaatgg ctttttggat ggtctcttcg tcgtaattca ctgattccgc
    20461 tgatgcagcc agatcaattg ccgcagccct cgtccaaatc aggttggaac gaataagctc
    20521 tgctttagag tcagcttgaa gaataagctc atttttcagc gtgtcgatca aagctgtttt
    20581 tgcggcttga atgctaaggt aggcagatgc aaacaaggcc agcaagagca gggtgatcac
    20641 cactacaggg agtttaactt gcagactcac atagcgacca aataaacctc tccgtggagg
    20701 tttgttcaat gacttggtgg atgtgggtag gctgcgagta cgcatttagc tttccttgag
    20761 agactgataa atattatttc aaacgaagtg aacttgtgtt tttacgaagc tgggttggct
    20821 caggttgtgg cgcaccaatg ctgatttcag cttccttgag gtcaaagact cggcgaagtt
    20881 cagtggttgc agtgcgaacc acagattcta cgtcgagggt ttcgcggacg cgggaggata
    20941 tatttgcgat gacctgattt cgcatcgcgt tcttttgagt ctcatccacc aggcggctat
    21001 tttccaatgc aagcgctact tgagcggcca ctttttcaac caggatacgt tcacgttcag
    21061 accacgttga cgagatgccc ttcctcttta ggacgatgtt gcctatgttt tgtccatgga
    21121 ggatcatggg gacatgaagc ccgtctgcct tctcggtttt aatttttgaa aaaagcggac
    21181 gtacacccgc aggagtatat tgatatgcag gtttgtggcg actggtaaat tttgaccaag
    21241 tctcacgggt ttgcagagta gtgttggatt caagctcata aaccaggctt tttgtctcat
    21301 cgatcaggcg gacattgtca atggaaatgg caacgagatc tgcaagagtt tgaagcacct
    21361 ctgcatcctg tccatcgaat gtccgtggtt gatcggagtg gatatccaga ataccaatca
    21421 cctgcccgcg gaccgtgagt ggcagcatga tacgcgatcg ggtcaacgga aaattggggt
    21481 cacggatgaa gttggcgtct cccacatcca ttgcgatttg cgggcgtctt tgctcaataa
    21541 ccagattggt catgtttaat ttatttggtt cgatacggaa gccctgaccg atcaggctct
    21601 ttcctgtagc ggaagatgag gcctgtaaaa acgccgtcct tttgcgttcg tccaaaagat
    21661 ataagcctga atgatagtag ccgaactcat ctgttatcag gcgggtggtt tccgacaata
    21721 actcagaaat atcctgaatg cccgcaatgg ttcgagttac gctcgttgct gcgcgcaatt
    21781 gaattgcccg tgcttcgagt tcttctgttc tttcacggac cctgtcttcc agtttttcac
    21841 gttcagtcgt taaagaattg aacgcgctct gcatttcgaa aatgacctgg gtcaacgcct
    21901 tcttaaattg atttgtagct acaaggatga ttgcactaac gatggaaaaa tcgaccgtat
    21961 aagcggccca atccgttgca atggtggggg ggacaccctt tgctgaaggt tcataaatgc
    22021 ccaattgctg tagtacgccc agtgttacaa aggttaatgc actgaggatg agagcgatca
    22081 agtcaatgcg attgtcgaag agcagggcag caaggatggt aaatgcgata aagaatatgc
    22141 tgccatccag ccagggaccc cacgaaagga gcgagtttgt cccaataata taaaccatga
    22201 aaagcaaact ggcggcgcgg acagaatatg gggtttttag aaccgtgatc gcgaggagaa
    22261 ttagataaag gctcggaaac aaaacacgat cagtggctga tgcagttgga aaggaggttg
    22321 cgatcaaagc cgcacccagg acgcaggcaa tacgcagaac agtaatgatg aactgttcgc
    22381 gccagcctat gtaatcgaac gcagaagagg tggtgattgg tgttttgcta ttcattgtca
    22441 tcatccatgg tcacttctat gtctacttct gcagcttcca gtgcgcgccc cagttctctc
    22501 actgtggttt ggaggatgct gtcaatcgtg gtggaggccc atacttttga ggtgatctcg
    22561 ttgatcagcc tttcacgtgt tgcaacagat tggctttctt ccaccaggcg cgcgttttca
    22621 agagcaaggg cagcttgtac tgccacagat tcgatcaaat tcttttgttc agaggtccag
    22681 tcacttgcgc tggataggct gatctgaccg attacctgat cacgcagtgt caatggaact
    22741 tcgatttcct gtttatcact ggattcgcta tcgcctacgg catattcgag tttttgttcg
    22801 gatgatagcg acagccaggc acctttcagg tattgctgtt gggtggcact catctcggca
    22861 aggctttgtt gcgcttcagt aaacaggcgg gaattttcga tcgcaatcgt gatctggttt
    22921 gccatgtttt gataggcatc gacatcttgc tgcatgaaag cattttcttt tgtagagtgt
    22981 agctcaagcg ccccgatgac acgatcgccg atggtcagtg gcagcgcaat ttgggagcgg
    23041 gtatagggga gaaggggatt atctgatcgg acaggatccg ctccagtgtc cagtgcgaca
    23101 cgggcctgtt ttgtccgcag taccgtgctg acaatgcttt tcccgttgac attcaaatga
    23161 tgcttgtttt caatcaaaac cctgccggcg tcaccggtgg cggctcttaa ttcagcccat
    23221 tggttggttg catcgacaat aaagatcgct gtgtaatagc attcgagttc gtcgctgatc
    23281 aaatacacgg catggctgag gagttcgttg gggtctagaa tggctgtgat ggcccgacca
    23341 acacgattga ccttttgcaa gagctgggtg cggtctgaaa cgctggtctc aaggttgtca
    23401 cgttcgtgtt tgagtgcaga gagagtggac tggatctgtt tttgtgtttc gaagatcgca
    23461 acttcgagcc gttgaaaacc gagcacaata acaacaccaa acaggatgat cgccgcacct
    23521 gcgctgaacc agtcgctaat gttggaaggg ggagcgtttg gattgatcgg tacgaggcga
    23581 ccactctgca ttgatatgcc aaccgcaaca aaagtcagaa tgttgacaat gatcgctgcg
    23641 atgcctgttt tgggagatac cataagggtt gcaaaaacga tcaacccaag gaagaagaat
    23701 aaactgtcac ctaaaattcc atgggtaaat agttctgcca atgcaagcgc aaaaatgctt
    23761 gtcaggaaaa cgctcatgcg gatggaatat gaaaaacgaa taactgtgac aatgcctgtc
    23821 aatacgtaaa ctgagataaa caggaccctg ataaatgtgt tgcccgcacc gcttaaagct
    23881 gggattaagg caaggatacc caacaccagc gacccgatca aaagaggcat ggtgaactgc
    23941 tcacgccagg atttgtacaa caggcgtgtc gcttcttccg ggctgttgct tgcgttggtg
    24001 gtggaaggat aactttccat catcaattac cttcctgtgt tgggtcattg ccgggttgaa
    24061 tacgaatctt agtgtattgt gcgccggtaa gccttgcaag ttcacttgcg gtcgtgttca
    24121 aaatggattg gatatcggtt gacctgcgga tcttgcttgt cacttcataa atcaaacgtt
    24181 ctcgttctgc ttgaatgcga atttgttcca acagacgggc gttggcgatg atggcagcca
    24241 aactgcctcc aagtgtgccg agcatttctt cgtcgttttc tgtgtacgca tccgtttgct
    24301 cgctttcaac gttcagaacg ccgagcagtt cattgcggta tacaagtgga attgccaatt
    24361 ctgagcgggt gtttgaacta atgcggatgt aacgaggatc gtcagaaaca tctttgatcc
    24421 taagaggtcg tttgtgagca gctgcccagc ctgtaatgcc gctgccaatg ggaacttgta
    24481 ctttggcaat atcttctgcg tagcccatgg aagccttgac ttcgagcagg tttttctcgc
    24541 ggttggccag catgatcgta acgcggtcgc caccaagggt aacctgaagg ccgttcactg
    24601 cgttttcaag cgcctcttca agggtggtgc ccgaagcggc agtggtggtg atgtgatgca
    24661 aaagacggtg ctgagagaga tgctcctgcg tttcagcaaa caattctgtg ttgaccacgg
    24721 caatggcgat ctggtcagca aggatctgca aactgcgtaa tttgtcttca gtaaacgcat
    24781 atggacgggt gctttgaaca tccaccacgc caagaatacg ttcaccaacc ttcaggggca
    24841 ctgcggcttc cgaccgtgta tcagggaaca gggggtttgc ataataagtg gcgtctttgg
    24901 tcgtatcgcc gacaacaaga cgttccccac ggctgcttac ataaccgacg attgattttg
    24961 aaccaatgcc gatcttatac cctgagcgtt tcatctgagc ccctgcttcg cctgtggctt
    25021 cgcggatgac cgcaaactcg cctggcaggt catgtagaaa gacagatgcg tggtaaaaat
    25081 cgaaacgttc acgaataaaa ttgacggctt tgataagaag ttcgtcaagg ttgagagatc
    25141 cgctaatgtc acgtgcaatt tcagcggcag tttccaattg gagcgccttg ttctggcttt
    25201 catccagaag gtgggcattg tggattaccc cagcgacctg gccagcaatg gtgttgaaga
    25261 atttcagatc ttcctcgtcg aaggcatgtt ctttttcagt atcttgaatg atcaacgcgc
    25321 cgactggttg attttgcacc aacatggggg cgcccagcca ggaacgtcct ggcttgcctg
    25381 aaaattttgc acccagttcg atcattcttc tttcggcatt ttccacgatc atcaacggtt
    25441 ggcgagtgcg gatcaagatg gaggtcaggc cttcaccgag cgggaaggat tcgattgagg
    25501 tgattttgtc atcttcgtag gcgaatggaa tactgaccgt gttatctctt tcattgtata
    25561 gcgcaacaat aaaggcatag tcgccgataa cctgtcggat cttggcatgc agattcatga
    25621 agaagctttg catatcggag gatgttgcga ccacttcgct gatcgaggca agtgcctcca
    25681 cttcacggag atgtttttcc gtttgttgga ttgcatccag cttttccatg gtcacggaca
    25741 tgaggtctgc cagattggca tatggctgta cgattgcatt tgaaaacaat tgctgcccac
    25801 ccatgataat gacagtatgc agcagaccac ctctccgcaa tggaaaaact gcagcggagg
    25861 agaatcggag tgtttgcata atgctgtcga gtgcggcggg aatgtttttg tcttcagaag
    25921 tgatgatcac ttgtccgttg tgcaggtagt tgtgaatgtc cacaacagac gcctcgctgg
    25981 cacttggcag gctgatcttt tttacgctgt ttccgctttt tccatcggtt tgtgagaaca
    26041 tctccagctt gttatttacc acccgtagaa taattacggg gtagggagca tctgcaaagg
    26101 cgtcgcgtcc aatttccagg atttcattct cggtattggc ttcggctatt aacctgctgg
    26161 agcgatatag ttgggcaagt tcttcaaaat tgatctggct ggtctctgtc agaccagtag
    26221 tttggatggc agtagcgatc tgactggcca aagtctgtaa catggcgacc gtttcagtgt
    26281 caaaaacgcc cacctgtatg ctttgtacat ccagaacacc caatacaaga ctgccaattg
    26341 agattggaag acaggcttca gaacgtgtct caggcagcaa ttcgtttttt aggtggatcg
    26401 gatcttctga aacgtcggag gcgatccgcg gctgattgtt ggtgcttacc caaccgacga
    26461 tcgattctga gtttacttcc agcctgtatt ttctttttat caaggcgctg gttgcagaac
    26521 cgaaccccgt tttgaactca agcgcctttc cgcctgggtc catcaagaaa acactggctt
    26581 gatagaagcc aaattcacgc acaagcagtt cgacggtttt atcaagcatt tcgtcgaggt
    26641 tcgaaagttt tgtaacactt tgcgccactt ccgcggcagt gcggatttga cgactgcgtt
    26701 cgtcaactgt ttgctccaat gagcgataca ggcttccaag ctcttcggcc atgtggttga
    26761 aggaactggc cagcaagcca acctcatcgt cgcttttgat ctcagcacgt tgactcaggt
    26821 caccgtctgc aaaactgtgg gcgatctgtg aaagtgactg caagggtttg atgacgcgat
    26881 taatagtgat tgccaatgca aggccgatgg ctaataaagt gatcgtagta agaattgcag
    26941 taaacggcgc cagactggtc aggcttttat atgcttcggt cgtgtcggtt tcaaggatca
    27001 tccaaacttg aagtttggga acccattgaa cttgaaagac tttgatcttg ttttctgcgg
    27061 ttgtggtttc aatggatctg ggtgagacgc cgccatcaag cctggactca gtcaaggcag
    27121 tttcaagctc tgattgagct tcaagaggag atgttaatag cgtaaaccga ttggcttcgg
    27181 gctggctctg gaagaattgc tggttggaaa gcaccaaata agctgaactc atcggcaggc
    27241 cggataggga ctgcatcgaa ttttggatgc ttttcttttc tgtgatccct acaacgaagc
    27301 ccagaacggc tccctgtttc gtctcatacc tgagggtggt gacaagaagc aactcctcct
    27361 cgtataaagg cgaaagcccg taaagggcca gggtgtgttg agtatcaaaa tcttgaccaa
    27421 tgattgctgg gtcgagcgtt tccccctgcc acttttcttc tgtggcaatt tttatgtttc
    27481 cattcgtatc cagtaagaga aattgatcga atgcaggcat gttctcgtgc cgattcatgg
    27541 cactaaactc ctgcagaatt ccatctctca ggacctgaaa ttcatccgtt ttggggtcgg
    27601 agtgcagtgc cagttccagc atgatctgaa aatcactgct tgccaataga tcctgaaaat
    27661 ggacttcctt gtctccaact tcacggttga tgatatttaa ttgtgataca aacaggtttt
    27721 gagcttggtt atttgcctgc tcacgaagca acgtacgcgt acggaaataa gccgttcccg
    27781 ccatgagagc gagagggatg aaagtaaaga ttaagagcgt tcgaacaaga gtacctgtca
    27841 gactgccctt gtatcttttt tgtttagagt ctaatccggg cgtcgtttga gtcgctgtca
    27901 ttgaaaaatt aactatactc ccaaacaggc tctccactca aaatgcggcg aatttgatcg
    27961 gggaaacggt cggggtcaag cggtttaccc aaaaagccat caaagccaga atctttggcc
    28021 tttgccattt gctccatact ggcttctgcg gtcacagcaa tgatcggcac cgctttgaaa
    28081 cgctcagaag cgcgaatctt ctttaatgcg ccatacccat cttcataggg caggcggata
    28141 tccataagaa taaggtcaag gcgggggagt gtgtcggcat actctaccac ttcatagcct
    28201 gatgtcttcc attcacaatg aattcccaga tacccgagca tgcgtgcaat caacacaaag
    28261 ttggaaacat tgtcctcaac aaccagaaca gccgcatctt ttgggatgtt tggtttccga
    28321 atcggttgag tatctgatat ggcagggatt tgatcagaca ttagaactcc ttgttatata
    28381 gcgctcgatc tgctgggtaa gcgtatcaat atcaatgggt ttttgaatat aaccatcaca
    28441 tcccgcttcc agtgattttt cgcgatcgcc gcgcatcaca tttgctgtga ctgccacaat
    28501 ggggattttt gtaaactttt gaactgattt gatctttgct gtaagggtat acccgtccat
    28561 atctggcata ttaatatcca tcaggaccag gtcaatgttg cctttttcaa ggttttcgag
    28621 tgcctgactt gcattaactg cttcgataac agaataatcc tcagattcaa gaaccctgcg
    28681 aatcaaactt ctgttatcag ggttgtcttc cacgtaaagt atagtacctt tttttgtttc
    28741 cggtatcata cgactgcctc cgacctgaat tttactgtaa gtcatgccta cctacataac
    28801 agcaacctta cagatggaat aaaaaatcga ggcaggcaaa tcctcgactt gttagagcgg
    28861 gtgatgggac tcgaacccac gatatcttgc ttgggaagca ag
//
LOCUS       DAOQLC010000049        28816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_519_length_28816_cov_21.393519, whole genome shotgun sequence.
ACCESSION   DAOQLC010000049 DAOQLC010000000
VERSION     DAOQLC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_519_length_28816_cov_21.393519"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1662)
                     /locus_tag="PL000_10280"
     CDS             complement(175..1662)
                     /locus_tag="PL000_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC89332.1"
                     /translation="MKRLEQLTFTRFIAIIVVLFFHGGGGVYLNAIDFFPLSPILVSA
                     TTSVTYLYVLSGFVMSLVNHRPGEKFNIGKYWTARFLRLYPLYLLSFLLVCYFYADSV
                     AQIKLQKTLANVLILQAWIPRYAQSFNFAAWSMTVEFFFYAVFPFFTMWAYRQPMKKL
                     IWGSVILWTVSQTIHNALWVGYFPEYENFLVYFPVFHFSSFILGAVGGIWYLREGRNH
                     DYKPSMTLPVFIGGIFLACTYIVVSSRVPGLPHGLQMMAGLLAPILTVVIVALALDKS
                     RLSVFFNTPALVTLGDSSYGLYILHVPFFWLYERMLNNLGIADPKRLFDLTFLPLTIV
                     LVLLIHFYFDQPIRTWLKRILQNVSMPLLALDLCALGLSIYLAFTLRVDMGRESDLYR
                     PIALAMFWSAFALRTVFTVAFQAANPAMLRGSLFEFIRPVLISVTVGTVALGAIVFAG
                     YWAGAFHNFPRSILITDWAIMLALSLLVRYLFRQAGVYAPRPMSV"
     gene            complement(1754..1984)
                     /locus_tag="PL000_10285"
     CDS             complement(1754..1984)
                     /locus_tag="PL000_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HNC89333.1"
                     /translation="MKAYIMIKIRAGDVKDVVLQLRKVGDVKEAHMTFGPYDAVAVVE
                     TSDLARLGSITALEIQPIPGVEQTLTCLAVDL"
     gene            complement(2000..2887)
                     /locus_tag="PL000_10290"
     CDS             complement(2000..2887)
                     /locus_tag="PL000_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HNC89334.1"
                     /translation="MSEIRIDALLPAEMATRAEYLGVRKAEMPAFTMFMLSLLAGAFI
                     SLGAIFATTVASGNMAVTAADGAVAYTTGLPFGVTRLLTGLVFCLGLILVVVGGAELF
                     TGNNLIVMAWASGKVTGRALLRNWAIVYAGNFIASIATAALMFFTKQYTFGSNAVGVA
                     ALRIAVAKCDLGFVQAVALGILCNALVCLAVWLTYSARTTIDKILAIVFPITAFVAAG
                     FEHSIANMYFIPIALFIKDFDPQFVAAVGDKVPNLELLTWQAFFIKNLIPVTIGNVIG
                     GAVLVAAVYWVVFLRDKKA"
     gene            complement(2991..3551)
                     /locus_tag="PL000_10295"
     CDS             complement(2991..3551)
                     /locus_tag="PL000_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC89335.1"
                     /translation="MRRFFTLILIPFLFLTLGLIPFQWVDATSVQPAQVSTAVVTMIS
                     GTPIPVIHTSTPMEDGAVYHVVQADEALWSIALAYNTSVEQLKLLNSLSNNAIFIGQT
                     LLIVRPEVSTETPTPTLTVTLGIPTSTVTRPVTATATVTPTPLPVAPISRRSSGMVLG
                     AIVIIALLGAGIGAWLGSKRKAVAAE"
     gene            3696..5117
                     /locus_tag="PL000_10300"
     CDS             3696..5117
                     /locus_tag="PL000_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNC89336.1"
                     /translation="MPFKRIPFKKRVLAKIELAIKGPMFGCRMCGNCLLQETAFICPM
                     ECPKGLRNGPCGGSTPEKCYVDESRPCIWYKIYERSFKLGREALLLEVLPPMDWDKAG
                     TDAWSDVFEQAKQVGVKKIITGLTSGETGSVSKTLDSVFRPVRQPEWWQGDSLYHAPK
                     YTEPVSELERRLKAGEFAVAAEVAPPLSANTDKLKQNIEMLKPYVAALNFTDSPSATP
                     RMSSWACSKIAVDHGADPIMQIAARDRTRVGLQGEVMGAEALGVRNILCLTGDSMALA
                     PSPRGRMDIVDLDGIQILWILRRMRDEGKYLDGRELKTPPKYFLGAAASPFASNMKFQ
                     ALREQKKVNAGAQFFQTNVVFDADALDEWLNEIAKRDILDKVYIMIGLSPLKSYRAAV
                     FMNDKVPGVFVPEKILKRMENAKAKGNEQEEGVQITLELIEKIKGKQGVSGIHLMAVG
                     WEEIVPRIVREGLGVAPATATIK"
     gene            complement(5164..5577)
                     /locus_tag="PL000_10305"
     CDS             complement(5164..5577)
                     /locus_tag="PL000_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC89337.1"
                     /translation="MTHIITSLCIRDRGCIEVCPVECIVPGQPVAEWPWMYIDPDTCI
                     DCGACVPECPYAAIFPEDEVPSAYSAKGGEYISKVGLTGHYEGTNHHGKAVVLETTRK
                     LDAGETVDLTPDIQPNYEFFKSGPGYSAKDSDQGS"
     gene            complement(5750..6355)
                     /locus_tag="PL000_10310"
     CDS             complement(5750..6355)
                     /locus_tag="PL000_10310"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNC89338.1"
                     /translation="MAFELPKLAYAYDALEPHIDARTMEIHHTKHHQTYITNLNGAVE
                     KTPELAGKSLEAMLSDLNAVPEGVRMVVRNHGGGTYNHNLFWEVMGPNAGGAPKGDLA
                     KAIDASFNSFDAFKDEFTKAATTRFGSGWAWLVKKGSGLAVVSTANQDTPLSDGLTPI
                     MGIDVWEHAYYLNYQNRRPDYITAWWNVVNWEAVSAKYAAK"
     gene            6483..7436
                     /locus_tag="PL000_10315"
     CDS             6483..7436
                     /locus_tag="PL000_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89339.1"
                     /translation="MQDSRARLLLPVIIPSAILAVSTASIFIRFTQAEGAPSLVIAAV
                     RLTIATLLLAPFALTNYLDEIKRFTRTEILLGAFSGIFLAVHFATWISSLEYTSVASS
                     AVFVSTGPLWVALLAPLLLKERLTKTAILGLILALAGGTVIGLSDACVWESGLICTDI
                     QNILQGRAMWGNILALIGALAVSGYLIIGRRLRNAGNMSLIPYIFLVYGMAAVTLNVI
                     MFASGHSPFGYSPKIYGLIFLLAAIPQLIGHSTFNWLLKFLPATFVAVTTLSEPIGSA
                     ILAYFILNETPTSFVIIGGCILLIGIYLTSREENNTSKESK"
     gene            7433..8698
                     /locus_tag="PL000_10320"
     CDS             7433..8698
                     /locus_tag="PL000_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HNC89340.1"
                     /translation="MTLQLSAKDQEMLKGQHGPATRMAMSILVRMAEVSGAKELLDIS
                     GAHIDSTVYIGDAGLEYAERLASLGAKVAVPTTLNVSGVDEHHWKEWAVPTLWAKQAH
                     RQMVAYQSMGTIPTWTCAPYQTEMRPRFGQQIAWGESNAIVFANSVIGARTERYPDMF
                     DVCCAITGRAPAIGLHLTENRAGKILLKLEGVSAELQASDDFYPVLGHYLGKISLDRI
                     PVIEGMTIVPDDDQLKALGAAAASSGAVALFHMVGVTPEAPTVEAAFQGNKPEETLIV
                     NMDTLRQARRELTHADDASLDMVVLGSPHFSLAEYKILAKLVEGKKKHADVKFLVTSS
                     RAMTQLAQQAGFLDPILQFGAQITVDTCILTTPMLPREIRFLMTNSAKFAYYAPGLLD
                     KRIAFSSLADCVTSAVKGEIVRDETLWQS"
     gene            8686..9105
                     /locus_tag="PL000_10325"
     CDS             8686..9105
                     /locus_tag="PL000_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HNC89341.1"
                     /translation="MAKLELFGKPVISGKANGIALVSREPLSFWGGYDWKSGEITDRR
                     HPLSGENASGKVLVVPFTRGSSTTTAVLLEAIREGTAPAAIITTATDFFFALASVVAG
                     ELYEKSFPLIALSEADFGAIKTGDQVKVMEDGKLIVE"
     gene            9154..9474
                     /locus_tag="PL000_10330"
     CDS             9154..9474
                     /locus_tag="PL000_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89342.1"
                     /translation="MSWLIVILIAVVVNIIVLATCGGGGGLMLLVALNGFSESAAMPF
                     LAGYGLLVLAISFGIATGATWIYVKPRSTASGIGFWQVAGLNAGIIVLLILGVVAFMA
                     IRAL"
     gene            9482..9964
                     /locus_tag="PL000_10335"
     CDS             9482..9964
                     /locus_tag="PL000_10335"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HNC89343.1"
                     /translation="MIIVVMGVAGSGKTTVGALLALELGWDFYDGDDFHSDANRAKMS
                     QRIPLNDEDRSTWLAALRDLINQNLSENRSIVLACSALKESYRHMLKVNEQVHFVHLQ
                     GSYQQIEERLRGRTGHFMSAEMLASQFDILETPEDALSINISNTPQEIVAIICKGLNP
                     "
     gene            9961..10458
                     /locus_tag="PL000_10340"
     CDS             9961..10458
                     /locus_tag="PL000_10340"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNC89344.1"
                     /translation="MKLPSHLYLDEKNIPYEALSFSPETEKGAANVAHALGFSERQAV
                     KTLIFQVDTGERVLVMLGGDQNAVSGNLKKAIGSRNIQMAAPDVVKETTGYVIGSIPA
                     FHWQPENFRSFLEASLLNEPILGTGGGQWGEEIMITPENLVKASNAIVVNLTDREKPV
                     FLESV"
     gene            10505..11767
                     /locus_tag="PL000_10345"
     CDS             10505..11767
                     /locus_tag="PL000_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89345.1"
                     /translation="MIGKSFPAKKTIVLVILSVAGGLLAVVNFFLLSKDMLRGDYSAK
                     DIFPQPMYGFFRVPDRPNTIAQNGVNRLAGDYAQVYFPSLEFSSLTQNYETGYLDPWK
                     RPSRYAPFIHYVCALSFCKLDYGYASLLHMLTQMVLFYAFFIAAFKILEIESDLWVGL
                     LITTLLLFVTPAGLSWFERGQFSLYVALAYLLLMLGLIKNKPVFVFISALFAYVKWTS
                     FPFLFVVLFVHWMSAKNTKERAQYTQSALIYLLVILVLSLAFRSRFIHFLEGIYKQEV
                     NVEAVGIALAQLLPTSLVKGMPILLILLGYLSLRKNNMDFERAAPFLIGSGILMLTYP
                     TVAFEYNIPNLFCFIPLIFYWARNPKLMNHAAKYVFFIFISLASCTAFLGSTVGAKTI
                     LFGYMAVAGFFLILPFIDNKTFGNALKG"
     gene            11775..12296
                     /locus_tag="PL000_10350"
     CDS             11775..12296
                     /locus_tag="PL000_10350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC89346.1"
                     /translation="MANPQIRIQTELGDMIVELYPAHAPITVENFLRYVDENRFEGAT
                     FYRVVHLENQPNNQVKIEVIQGGLKDEDHPQSLPNIAHETTTQTGILHRDGVISMARN
                     EPGTASADFFICTGDQPELDFGGRRNPDGQGFAAFGKVIAGMETARRIQALPEENQYL
                     KTEIAFKIQRAQP"
     gene            complement(12374..13879)
                     /gene="malQ"
                     /locus_tag="PL000_10355"
     CDS             complement(12374..13879)
                     /gene="malQ"
                     /locus_tag="PL000_10355"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNC89347.1"
                     /translation="MTFKRSSGILLHPSSLPSPYGIGDLGPQSFRFIDWLASTGCKLW
                     QVLPLGPTGYGDSPYQAFSAFAGNPYLISPDELFADGLLTQEDLDGMKDLPASHVDFG
                     LFIPRKLDLLQKAFSRYQSNPEHLRQAFDYFCAENASWLDDYTLFMALKEANGGGAWN
                     GWPEALRKRKKAALSKAQKELGESVMRFAFYQFIFFRQWNKLRAYANERGIQIIGDIP
                     IFVAYDSSDVWSHPDLFFLDREGNPTVVAGVPPDDFSATGQLWGNPLYKWDVHKKEGY
                     AWWLSRVRSSLQIFDILRFDHFRGFAGYYEIPADHTTAEFGRWVTGPGKDLFRAVDKF
                     LGDGLITHGTGLPIIAEDLGLVTPDVIELLAAFDLPGMKVLQFAFSGPENPFLPHNYV
                     PNSVAYTGTHDNDTARGWFEHAAPHEREFAQRYLSVDGNDFAWDLIRGVWKSVAVFAI
                     TPMQDLLNLGSEARMNFPSKLGGNWEWRMGEYDLREDIAGKLRDLNWLTYR"
     gene            complement(13876..15300)
                     /locus_tag="PL000_10360"
     CDS             complement(13876..15300)
                     /locus_tag="PL000_10360"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNC89348.1"
                     /translation="MPKTINVLFLAAEAEPFIKVGGLGDVAGSLPRALRALSNDEITL
                     DVRLVLPYHPAIKAENLRPIGIFSIPRGNSEVQVDAFETSLKGMPVYFINGDAIRANG
                     SVYSLDAKLDAEKYSFYSIAALELSRQINWFPDVVHGNDWHTALSLYYSLTKRWEEGA
                     RHVASVLTLHNLPFMGPDVSGILESYGLKLAQTDLPDWARVMPLPLGLWSSDAIVGVS
                     PTYAKEVLTEEYGCGLNEFLQSRRETLSGILNGLDTDSFNPADDPAIGVNFALETLEK
                     RAANKGLLQARLGLPQDPEIPLFAIVSRMDVQKGVDLAFNALNTMKRLNWQAVILGTG
                     DPKLEEAAMKLQKLFPEKVKVETRYDAGLARQIYAGSDMFLMPSRYEPCGLSQMIAMR
                     YGCVPIVRAAGGLNDTVRHGVTGFVFEKAHHLSLVAAVKNALKVFANREQWQSLQRSG
                     MAQDFSWENSARQYLNLYLSLIKS"
     gene            complement(15361..15432)
                     /locus_tag="PL000_10365"
     tRNA            complement(15361..15432)
                     /locus_tag="PL000_10365"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15398..15400),aa:His,seq:gtg)
     gene            complement(15464..15928)
                     /locus_tag="PL000_10370"
     CDS             complement(15464..15928)
                     /locus_tag="PL000_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89349.1"
                     /translation="MTISLHPVSAENWRALISLKVREDQTHFVASNLYSIAEAQFGFE
                     DEGHWEMFPFGAYAGEEPVGFLMYCLNFTHSRFQAFIARLMVDEKFQGKGYGRDIMSK
                     ALDRLRADERIRNVGISYEPENESARKLYASLGFEEPGEMYDGETLAVLNLR"
     gene            complement(15933..17204)
                     /gene="hisS"
                     /locus_tag="PL000_10375"
     CDS             complement(15933..17204)
                     /gene="hisS"
                     /locus_tag="PL000_10375"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNC89350.1"
                     /translation="MASKIQPVKGTREFYPEQMALRNFIYEKVRMASQKFGYQEWDAP
                     FIETIDLYAAKSGEELVKKQSFTFTDRGGDFVTLRPELTPSLARMIAAKQGELTFPVR
                     WWSFGPCWRYEQPQKGRTREFFQWNIDMLGVNSPEADAELIAVAATFLRSVGLTPERA
                     AIYVNNRRLMDSEFDALGLAPERRLDVSNLVDRRTKMDPAKWDSYALELGLTQTQLDG
                     LKGILGNFDLWKNSEELVRLFSALKALGVSEYVKFDPNIMRGLLYYTGTVFEAFETSG
                     SLKRAILGGGRYDNLLADVGGQPLSGVGFAMGDVVAGIVLQEAGLVPEFAPTPAAVLV
                     TVFDEAMWLKSFELAAQLREAGINAMVFPEPAKLQKQFKFADKMKIKLAVTVGPDEAA
                     NGQAAVKNLMTGEQVTVQREAVVSEIRKMIG"
     gene            17367..18167
                     /locus_tag="PL000_10380"
     CDS             17367..18167
                     /locus_tag="PL000_10380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89351.1"
                     /translation="MKIDYQEPSKDLLMRIDIHEKYGSANIDVWTNDLLKPQAGMKIL
                     DVGCGAGKLCFLFNEFTKGGAQITGGDFSNELLDKAREKNKTVGANIDFQFLDFNQRF
                     NFADNTFDLCTSAFAIYYASDLDFTFGEAHRVLNPGGRLFVSGPLPENKQMFYDIIKE
                     ATGAPIPPMPGSSRFKGDIFNTIDKIFAKTELHKFENHLTFPEVAPFIDYVRASLGED
                     RRLWTSMFNGKDEYEALIGKIEAVATRWFERDGKLVMTKVVGGILATK"
     gene            18230..18862
                     /locus_tag="PL000_10385"
     CDS             18230..18862
                     /locus_tag="PL000_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC89352.1"
                     /translation="MNFFGKRVLFLGAHPDDIELGCGALLHKIAKKTDVLCVTLSDNQ
                     KNPDLQKVKSEHFQSMQVLDVPEEKIVLGPFTTRVFPDARQEILEYFLKLRKDFNPDL
                     IFTHTRQDVHQDHNTMTDEALRAFRGITVLGFDVVRSSYGFFPSFLVEVSEENAAAKI
                     EALSKYETYRDRYYFNAELTRSIMVRHGALAERPLAEGFDILRIVGKFGE"
     gene            18945..19646
                     /locus_tag="PL000_10390"
     CDS             18945..19646
                     /locus_tag="PL000_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC89353.1"
                     /translation="MQKYERAVFISYAWGDEREEIVNQLDKALQKRGVKIIRDKRELE
                     YKGSISEFMERIGRGDCVIVVISDKYLRSPNCMFELIEIAENKKFRERVFPIILSDAN
                     IYDPVKRVEYVDFWENKRDELARAIQKLDPANLQGIREDMDLYDRIRDMISGLASTLK
                     DMNALSPQTHRDTDFEDLYNAIEQRLGQPQSARPKKPSKPAKIEKDETGTRNIEIGGS
                     ASGNVFIGDNNKIEK"
     gene            19680..21944
                     /locus_tag="PL000_10395"
     CDS             19680..21944
                     /locus_tag="PL000_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89354.1"
                     /translation="MANVSIGGNVTDSVLVFGNNNYVVKIGDVNGGVVNIVKPSDKPK
                     VSARPAPVMIKPRAFPSLMDRVDEAETVKKAAELAVPVSVWGKQGIGKTSFIRHLAHI
                     LPTNKYPNGVVYLNVSSLGHEDLLQALFDAFFESDNTYKPTTADILHGLQKISALIFL
                     DDLQIGRDETISILNAAPSSTFILISSERSFWGEGEAVQLQGLPVGDSVRLFEKELAR
                     PLETEEKITVPKICKMLQGHPLSILQSASLARESGKPLEEILNGLTNEKKDDKSMAYM
                     GLASLTDSEKQVIALLAAAGGTIVLAEHIKGIFKGDSRQTDIQKLIQLGFVQAHSPKF
                     SVTEAFASSLAATWDLSSWHDILLKYSIKWLSQQPASALVDESTNLLIHTIKTAGERK
                     KWSEVIQLGRALEKFLVYYKRWQAWADILKLIIEAAKALSDSKTQAWALHQLGTRALY
                     LGQTSEANSLLTQAQTIRKAIGDKAGLRVTQHNLKTIKGGSGCAKFVSYFVGAVVGAT
                     VLTGIAWLAINVLFSPPIPIALPTATNTPTLRPSSTIPPSNTPRPSETPTVRPSRTPR
                     PTDTPMPVLLYDFVESAHEVYWYQTTSNSDGEFTQDLPFFFEPVVPDVESYLPGSDSA
                     YVGWSFDTQLTNGNRYEKVLLTYPLYEHFKVFGDYTVKIGRPAPEAYLELTAGFKDIV
                     IEPVEGVMFRVYVNGKVYIEEPYYFGSEPIQTGSRIRLAANTYEFRLEVESLDPSPYD
                     FATWAVVRLWDKEP"
     gene            complement(22073..22420)
                     /locus_tag="PL000_10400"
     CDS             complement(22073..22420)
                     /locus_tag="PL000_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HNC89355.1"
                     /translation="MFNYTALDESKVEFLEIAPESELPNGERLFVDLGDKPIVIFNIA
                     GQFFAIGDICTHDDGPLGDGVIEDHNIVCPRHGAEFDVRTGKVMSMPAVVDIPAYPVQ
                     VRDGIIFIGVPKE"
     assembly_gap    22435..22534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(22540..22935)
                     /locus_tag="PL000_10405"
     CDS             complement(22540..22935)
                     /locus_tag="PL000_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HNC89356.1"
                     /translation="MDDLYREVIIEHYKHPSYRGKLDPHDISFADNNPLCGDHIQIDL
                     RVDAGGLVTDARFDGHGCAISQASADLLMETIIGKPLEEVKKLNKDSVLEMLGIDLGP
                     VRLKCALLSLKVLKAGVYGLGEASDSLVE"
     gene            complement(22968..24194)
                     /locus_tag="PL000_10410"
     CDS             complement(22968..24194)
                     /locus_tag="PL000_10410"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HNC89357.1"
                     /translation="MKLNVSQVRKDFPILEREIRAGVGLTYLDSTATSQKPLAVIEAM
                     NEYYRLSNANIHRGVHALAEEATAMYEGARERIAKFINAASAREVIYTRNTTESINLV
                     AYSWARANLKQGDLVILTEMEHHSNLVPWHMLQAERGIELEFIPVTEDGVLDLEVYKT
                     LLDRRPKLVSFTHMSNVLGTINPAAEMIRLAHAVGAITLVDAAQSVPHLTVDVQALDA
                     DFLAFSAHKMCGPTGIGALYGKTALLEAMPPFLGGGDMIKEVKLRSFRANTLPHKFEA
                     GTPAIAEAIGFGAAVDYLSKVGMDAIAAHEHEITEYALERLEEIPGVKLFGPSADKKG
                     GVAAFTLEGVHPHDVAQILDQDGIAVRAGHHCAQPLHEKFGIPATSRASFYLYSTKEE
                     VDLLVNGIYKVKEMFG"
     gene            complement(24234..24836)
                     /locus_tag="PL000_10415"
     CDS             complement(24234..24836)
                     /locus_tag="PL000_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89358.1"
                     /translation="MAVKSNSKRMVKPSAKAKTARTVSKKRVAVKTKTTVKTKVIKTE
                     VKKKPMQASKVVTAAPKKEVEQQTSNHPTAVKPAVTPVIKRVVNQMVERRRPVARPSR
                     TNQFSFMKAPGAEHDYEVGGSVEVFCDHEKNRERIRGWVKGIVVQVDNKMVAVQFRSN
                     VYLTDGWMVPDRILWFPLNSEHMRPVPGKKPPVKDFIPEY"
     gene            complement(24985..26322)
                     /gene="sufD"
                     /locus_tag="PL000_10420"
     CDS             complement(24985..26322)
                     /gene="sufD"
                     /locus_tag="PL000_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HNC89359.1"
                     /translation="MQEKPKVTVTRQRRADTAAKEFAFTQADVSAAGSLASFRASAWE
                     AFNKHSLPVNTDEAWRRTDLRLLPASDFKLPKIGAFDDLPAVPEYLLQPLTGEQHGGQ
                     ITLLPGGSKVDLDESLVKKGVVFTDFRTAEKNHADLLAKLIGQIVKPDDGKFAALASA
                     LAQNGVLLYVPRNVQVEQPLHSVLWGPGSELAHFSHLLVYVEDGASVTYVHEAASPDE
                     VGHAMHAGVVEIYVGKDANLRFVELQSWGRHVWNFSHERARVERGGNLDWIFGAVGSR
                     LTKNFSDLDLAGEGSVGRMSGFYFTDGTQHLDHDTQQNHLAPHTTSDLLFKGALKGKS
                     RSVWQGMIYVAPGAQKTDGYQANRNLVLDDKARADSIPGLEILADDVRCTHGATVGKL
                     EQEPLFYLKSRGIPQREAERLVVEGFFDPIMQRIPFEGVRERFQQAIQDKLSA"
     gene            complement(26390..27799)
                     /gene="sufB"
                     /locus_tag="PL000_10425"
     CDS             complement(26390..27799)
                     /gene="sufB"
                     /locus_tag="PL000_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HNC89360.1"
                     /translation="MSEDTKILDEIGEYKYGFHDRDDNYTFKSEKGLSREVVENISRM
                     KGEPKWMLEFRLKALEHFLARPMPNWGPELSSLDLDNIYYYVKPTEKSEKSWDDVPDD
                     IKRTFDKLGVPEAEQKFLAGLGAQYESEMVYHSIQEHLEKQGVIFLSIEDGLRQHPEL
                     FREYFGTVVPIEDNKFAALNSAVWSGGSFVYVPKGVKVDLPLQAYFRLNTANVGQFER
                     TLIIVDEGAQVHYVEGCTAPQYTTDSFHSGVIEIIVKKGARSRYSTIQNWSTNVYNLV
                     TQRAKVFENGTHEWVDANLGSKVTMKYPSCYLMEPGAHGEMLSMAFAGTGQIQDAGSK
                     MVHFAPNTTSKITSKSISKNGGRASYRGLLKVYKGAKGVKSNVVCDALLLDPKSRSDT
                     YPYIEIDEDDVTIGHEASVSKVGEEQLFYLMSRGLSEEEATTMVVSGFIEPLVKELPM
                     EYAVEMNRLIQLQMEGSIG"
     gene            complement(27873..28649)
                     /gene="sufC"
                     /locus_tag="PL000_10430"
     CDS             complement(27873..28649)
                     /gene="sufC"
                     /locus_tag="PL000_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002514361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HNC89361.1"
                     /translation="MAQLEIKDLHVSIEDKEILKGLSLTIKQGETHAIMGPNGTGKST
                     LAYTLMGHPNYTVTSGEVLLDGENILEMEPDERSRAGVFLAFQYPVAIPGVTVANFLR
                     SAINSRRRAENPEDKGMPIPEFRKMLKEKMDMLKMDHNFAGRYLNEGFSGGEKKRAEI
                     LQMATLKPRFAVLDETDSGLDIDALRIVSEGVNALSGPELGVLVITHYQRLLNYIKPH
                     FVHVMMNGRIVESGGPDLALHLEDQGYDWVREKYDAVAEG"
BASE COUNT         7518 a         7314 c         7018 g         6866 t
ORIGIN      
        1 tgtctccgcg gttcaaaatg gttttccaaa aacattccgt gcaacttatg caagatacgg
       61 gcaagttaca aaaaggccga ccctgtaaaa cgggtcggcc tttttgtgta aattcgcggc
      121 taaagtattg aaaaaacaat ttcgggatgt ttcatatttg cgagcccaaa acacttacac
      181 agacatgggt ctgggagcat ataccccagc ctgcctgaac aaataccgaa ccaacaggga
      241 aagcgccagc atgatcgccc aatcagtgat caaaatactg cgcggaaaat tatgaaatgc
      301 gccagcccaa tagccagcga aaacaattgc acccaaagca acagtcccaa ctgtgacaga
      361 gatcaaaaca gggcggataa actcaaacag tgatccgcgc aacatggcag ggttcgctgc
      421 ttgaaaagcc acagtaaaca ccgtccgcag ggcaaaagct gaccaaaaca tcgccagcgc
      481 gatcggacgg tacagatcag attcgcggcc catatccacg cgcagggtga aggcaaggta
      541 aatggaaagt cccagcgcgc aaagatccag cgccaggaga ggcatgctca cattttgtag
      601 aatccgcttc agccaggtgc gaatcggctg gtcaaagtaa aagtggatca acaaaacaag
      661 gacgatcgtc aaaggcagga aggtcaggtc gaaaagccgt ttcgggtcag cgatacccag
      721 gttattcaac atgcgctcat acagccagaa aaatggaaca tgcaaaatgt ataacccata
      781 agaagaatcg ccgagagtga ccaacgcagg ggtgttgaag aaaaccgaaa gacgagattt
      841 atccaacgcc agcgccacaa tgacaacggt cagaatcggg gcgagcagtc cagccatcat
      901 ttgcagtcca tgcggcaaac ctggcaccct gctgctgacg acaatataag tacatgccag
      961 aaaaatgccg ccaataaaaa caggcaatgt catgctgggt ttgtaatcat ggtttcgccc
     1021 ctcacgcaga taccagatcc cacccaccgc ccccagaatg aacgaactaa aatgaaacac
     1081 agggaaataa accaggaaat tttcgtactc gggaaagtaa ccgacccaaa gcgcattatg
     1141 gatggtctgg ctgaccgtcc ataaaatgac cgagccccag atcaatttct tcatgggttg
     1201 acggtacgcc cacatggtga agaaagggaa taccgcataa aagaaaaact ccacagtcat
     1261 ggaccaggct gcgaaattaa aagactgtgc gtaacgggga atccacgcct gcaggatcag
     1321 aacatttgca agcgtttttt gcaatttgat ctgggcaacc gaatcggcat agaagtaaca
     1381 aaccaacaga aacgacaaaa ggtacaacgg atataaacgt aaaaaacgcg cagtccagta
     1441 tttaccaata ttgaactttt cacccgggcg atgattcaca agcgacatga caaagccaga
     1501 aagcacgtac aagtaagtga cactggtggt ggcggaaacc aggatcggtg aaagcggaaa
     1561 gaagtcaatg gcgttcaggt acacaccgcc gccaccgtgg aagaacagca ccacaatgat
     1621 cgcaatgaaa cgggtaaagg ttaattgttc gagtcttttc atccaaatcc tcaaggaact
     1681 gcgcctattt tactggaaaa aatcgggctg gcatcagcca gcccgatggg gaaaacaggg
     1741 ggaacttcca agtctacaag tctacagcca gacaggtgag ggtttgctcc acacctggaa
     1801 tgggttggat ctccaaagcg gtgatcgagc ccagcctggc cagatcagag gtttcgacaa
     1861 ctgccaccgc gtcatacggg ccgaaggtca tgtgcgcctc ttttacatcc cccaccttgc
     1921 gaagctgcaa aaccacatct ttcacatcgc ccgcgcggat cttgatcatg atatacgctt
     1981 tcatggtttc tccttttgtt tatgctttct tatcacgaag gaacacgacc caatagacag
     2041 cggccaccaa caccgcaccg ccgatgacat tgccaatggt aacagggatt agatttttaa
     2101 tgaagaaagc ctgccatgta agaagttcaa gattgggaac cttatcgcca accgctgcaa
     2161 cgaactgcgg gtcgaaatct ttgatgaaaa gcgcgatggg gatgaaatac atgtttgcaa
     2221 tgctgtgctc aaaacctgcc gcaacaaagg ctgtgatcgg gaaaacgatc gccagaatct
     2281 tgtcaatggt ggtgcgtgcg ctgtaggtca gccacaccgc cagacacacc agtgcattgc
     2341 ataaaatgcc caaagcaacc gcttgcacaa agcccaggtc acatttggct acggcaattc
     2401 gcagcgcagc cacgccaacc gcattggaac caaaagtata ttgtttcgtg aaaaacatca
     2461 aggcggcagt tgcaatggaa gcgatgaagt tccccgcata aacaatggcc caatttcgca
     2521 ataacgcccg tccagtcacc ttgccactgg cccaagccat cacgatcaaa ttattccctg
     2581 tgaataactc cgcgccacct acaaccacaa ggatcagacc aaggcagaaa accaacccgg
     2641 tcaacaggcg tgttactccg aaaggcagcc cggtggtata ggccactgcc ccatccgctg
     2701 cggtaacggc catgttccca gaagcaacgg tcgttgcaaa gatggcgccc agtgaaataa
     2761 acgcaccagc cagcagggaa agcatgaaca tcgtaaaagc aggcatttcc gctttgcgta
     2821 caccaaggta ctcggcgcgg gtggccattt cggcgggtag aagtgcatca atccgtatct
     2881 cactcataaa tactcctttt caagaactga tgttaacttt tccacacttt cactatatca
     2941 aagcttggtc ttcaaaatca aagtgggttt gtaatggaaa gcaggtgatg ttactctgct
     3001 gctacggctt tccgcttgga acccaaccat gcaccaatgc cagcaccaag gagcgcaatt
     3061 ataacgatcg ctcccagaac cattccgctt gaacgacggg agatcggcgc cactggcaac
     3121 ggagttggtg tgaccgttgc ggttgcagtt acgggccgtg tcaccgtgga agttggaatc
     3181 cccaaagtga ctgtaagggt tggagtggga gtttctgtgc tcacttcggg tctcacgatc
     3241 aacagtgtct gtccgatgaa aatcgcattg ttgctcaggc tgttgagcaa cttcaactgc
     3301 tccaccgagg tgttataggc aagcgcaatg ctccacaaag cttcatcagc ctgcacaaca
     3361 tgatagaccg cgccgtcttc catgggcgtg ctggtgtgga tcacgggaat gggagtgccc
     3421 gatatcatgg tcacgaccgc cgttgaaact tgagcaggtt gtaccgaggt ggcatccacc
     3481 cattgaaacg gaatcaggcc cagggttaag aaaaggaacg gaattaatat caaagtaaaa
     3541 aatcgtcgca ttcaaaaatt ataccgtccc cggcacacat ccaaggactt ctcaaattca
     3601 ccagtgcggg tgtaaaatta tatataatat ataatcccaa ggagaccgtt cccatgcccg
     3661 gctttactcc aggacgccgc tttcaaccgc cgtttatgcc tttcaagcga ataccattca
     3721 agaaacgtgt tcttgcaaag atcgaattag cgatcaaagg ccccatgttt ggatgtcgca
     3781 tgtgcgggaa ttgtctcttg caggaaaccg catttatctg ccccatggag tgcccaaagg
     3841 gattgcgtaa cggaccgtgc ggcggctcga ctcctgaaaa atgttatgtg gacgagtcgc
     3901 gtccatgcat ttggtataag atctacgagc gttctttcaa actcggccgc gaagctttgc
     3961 tgctcgaagt acttcctcca atggattggg ataaggcggg aaccgatgcc tggagcgacg
     4021 tatttgaaca agccaaacag gtcggcgtga aaaagatcat cacgggtctt acttcgggtg
     4081 aaactggttc tgtttcgaag acgcttgatt ctgtctttcg gcccgtacgc cagcccgagt
     4141 ggtggcaggg cgattctctt taccacgccc caaaatatac agaacctgtt tctgaattgg
     4201 agcgccgcct taaagccggg gaatttgccg tggccgccga agtcgcccct cccctttcag
     4261 caaacacaga caagctcaaa caaaacattg aaatgctcaa gccttatgtt gctgcgctga
     4321 atttcaccga cagcccttcg gctactccgc gcatgtcatc gtgggcatgc agcaaaattg
     4381 cagtagatca tggagccgac ccgatcatgc agatcgcagc ccgtgaccgc acacgtgtgg
     4441 ggttgcaggg cgaggtcatg ggcgccgaag cccttggcgt tcgcaacatc ctgtgtctga
     4501 caggcgatag catggcattg gccccctctc cacgcgggcg catggatatt gttgacctcg
     4561 acggcattca gatcctatgg attctgcggc gaatgcggga tgaaggcaag tatcttgacg
     4621 ggcgcgaact caaaacaccc ccgaaatatt ttctgggtgc ggcagcttca ccctttgctt
     4681 ccaatatgaa gttccaggca ttgcgcgaac agaaaaaagt gaacgcaggc gcacaattct
     4741 ttcaaaccaa tgtcgtcttc gacgctgacg cacttgatga atggttgaac gaaatcgcca
     4801 aacgcgatat tctcgacaag gtttacatca tgatcgggct gtctccgctg aagagctacc
     4861 gtgccgctgt tttcatgaac gacaaggtgc caggagtatt cgtccctgag aaaattctca
     4921 aacgcatgga aaatgcaaag gccaaaggca atgaacagga agaaggtgtg caaatcacac
     4981 tggaactgat cgaaaagatc aagggcaaac aaggcgtaag tggaattcac ctgatggctg
     5041 tgggatggga ggaaatcgtc ccacggattg ttcgcgaagg gttgggagtg gcacccgcaa
     5101 cggcaacaat caaataaaca aaaaagccag gcaagcgcct ggcttttttg ttgcgatgat
     5161 tgattacgaa ccctgatcgc tgtctttggc ggaataaccg gggccgctct tgaagaactc
     5221 atagttcggc tggatatccg gggtcaggtc gacggtttcg ccagcatcaa gctttctggt
     5281 ggtttcgagg acaacggcct tgccgtggtg gttggtacct tcgtagtgac cggtgagacc
     5341 gaccttgctg atgtattcgc cgcccttggc agagtaagcc gaaggtactt catcttctgg
     5401 gaagatggcg gcatacgggc actcgggaac gcaagcgccg cagtcgatgc aggtatcggg
     5461 gtcgatatac atccacggcc actcagcaac gggctggccg ggaacgatac actccacggg
     5521 gcatacctcg atgcaaccgc ggtcacgaat gcacaggctg gtaataatat gtgtcatggt
     5581 aatttactcc ttgtaggatt gtaattaaac cgaacaaacc tattataact tcgtttatct
     5641 ttgtgacaag ggacacagtg gaaagtaaga cgccccctca aaagataagt ttgaaaaaac
     5701 atcccgcaga aatctgcggg atgttttgaa atggattgtc cttacgctgt tattttgcag
     5761 cgtatttagc ggaaaccgct tcccagttga ccacattcca ccaggcggtg atgtaatcgg
     5821 ggcggcgatt ctgataattg aggtagtagg catgttccca cacatcaata cccatgatcg
     5881 gggtcagacc gtcagaaagc ggagtatctt gattggcagt ggaaacaaca gcgaggccgc
     5941 tgcccttttt tacgagccag gcccagccag aaccgaagcg ggttgttgcg gctttggtaa
     6001 attcatcctt gaaagcgtca aatgaattga aagacgcatc gatcgctttg gcaaggtcgc
     6061 ctttcggagc gccgcctgca ttgggtccca tcacttccca gaacaggttg tgattgtagg
     6121 taccgccgcc atgattgcgc acaaccatgc gcacaccttc aggaacggca ttcaggtcac
     6181 tgagcatcgc ttcgagggac ttaccagcaa gttcaggggt cttttccacc gcgccattca
     6241 agttggtgat gtaggtctgg tggtgtttgg tgtgatggat ttccatcgtg cgcgcatcaa
     6301 tgtggggttc aagcgcatcg tacgcatatg caagtttggg aagttcaaaa gccatgtggg
     6361 ttctcctttg agttgataat ttcgtttgaa tgtataattc gattttaccc ccttataaca
     6421 atacccgcaa gaaatttgac aatttttata caaatatcat ataaatcaac cccagagagt
     6481 caatgcaaga ttcccgtgcg cggctgttgt tgccagttat cattcccagt gccattctcg
     6541 cggtctcaac cgcgagtatt tttattcgat tcacccaagc cgaaggggca ccttcactgg
     6601 tgatcgcagc agtcagattg accattgcga ctctcctgct ggcgcctttt gcattgacaa
     6661 actatctgga cgaaataaaa cgcttcacac ggaccgagat tctgctcggt gctttctcgg
     6721 gcattttcct cgccgtacat tttgccacat ggatctcgtc tctggaatat accagcgtgg
     6781 caagttcggc ggtcttcgtc agcacggggc cgctttgggt ggcgctgctg gctccgctct
     6841 tgctgaagga gcgtctgaca aagacagcca ttctcgggtt gatccttgcc ctcgcaggcg
     6901 gaacagttat cgggttgtca gatgcgtgtg tctgggagtc gggactcatc tgtactgata
     6961 tccaaaacat cctgcaaggc cgtgctatgt ggggaaatat cctcgcgttg attggggcct
     7021 tggcggtcag cggatatctc atcatcggca ggagattgcg caatgctggc aacatgtcac
     7081 tgatccctta tatttttttg gtgtatggca tggcggcagt gacattgaac gtcattatgt
     7141 tcgcctctgg tcactcccct tttgggtatt cccccaagat atacggattg atttttctgc
     7201 tggcggctat tccgcaacta attgggcact ccacattcaa ttggcttttg aaattcctgc
     7261 ctgctacttt tgtggcggtg accactttgt cggaacccat cggctcggca atcctcgctt
     7321 attttattct gaatgaaacc ccgacttcat ttgttatcat aggcgggtgc attctcttga
     7381 tcggtattta tctcacttcc agagaagaaa acaacacatc caaggaatca aaatgaccct
     7441 ccagttatca gcaaaagacc aggaaatgtt gaaaggccag cacggcccag ccacccgcat
     7501 ggccatgtcc attttggtac gcatggcaga agtttcaggt gcaaaggaat tactcgatat
     7561 cagcggcgca catattgaca gcactgttta catcggcgat gcagggcttg aatatgcaga
     7621 acgcctcgcc agccttggtg caaaggtggc cgtaccgacc acgctcaacg tgagcggcgt
     7681 ggatgagcac cactggaagg aatgggctgt gcctacgctg tgggccaaac aggcacatcg
     7741 tcaaatggtg gcatatcaaa gcatggggac catcccaaca tggacctgcg ccccgtatca
     7801 aaccgagatg cgtccacgct tcggtcagca aattgcgtgg ggagaatcaa acgccattgt
     7861 atttgccaac tccgtgatcg gcgcacggac ggaacgatac cccgacatgt tcgatgtgtg
     7921 ctgtgccatc acaggccgcg ctcctgccat cgggctacac ttgacagaaa accgagcggg
     7981 gaagatcctg ctcaagctcg agggggtttc ggcggaatta caggcaagtg acgactttta
     8041 cccagtactc ggtcattact tggggaaaat ctcattggac agaatccccg tgatcgaggg
     8101 aatgaccatt gtcccagatg acgatcaact taaggcattg ggtgcagcag cagcttcttc
     8161 tggtgcagtg gcactctttc atatggtggg tgttaccccc gaagcgccga ctgtggaagc
     8221 ggcctttcag ggaaataaac cagaggaaac cttgatcgtc aacatggata ctttgaggca
     8281 ggcgcgcagg gaattgaccc atgctgatga tgccagcctc gacatggttg tattgggcag
     8341 tccgcatttt tctttggcgg aatataaaat ccttgcgaaa ttggttgagg ggaagaagaa
     8401 acacgcagat gtgaagtttc tagtcacctc cagcagggca atgacccagc ttgcacagca
     8461 ggcagggttt cttgacccca ttctgcaatt tggcgcgcag atcaccgtag atacctgcat
     8521 cctcactaca ccgatgctgc caagggaaat ccgctttttg atgacgaatt cagccaaatt
     8581 tgcgtactat gctccaggct tgctcgataa aaggatagca ttcagcagtc tggctgattg
     8641 tgttacctcc gctgtaaaag gcgagatcgt tcgggatgag acactatggc aaagctagaa
     8701 ctttttggga aacccgtcat atcaggcaag gcaaacggga ttgcactggt cagccgtgag
     8761 ccgctttcat tttggggcgg atacgattgg aaaagtggag agatcaccga ccgcaggcat
     8821 ccgctttcgg gtgaaaatgc cagcggtaaa gtattagtgg tgccattcac ccgcggttcg
     8881 tcaaccacga ccgctgttct tttggaggcc attcgcgagg ggactgcccc cgctgcaatt
     8941 atcacaactg caaccgattt tttcttcgcc ctggcttctg tggtggctgg cgaattgtac
     9001 gaaaaatcct ttccactgat cgctttgtca gaagcggatt ttggcgcgat aaaaactgga
     9061 gatcaggtta aagtgatgga agatgggaag ttgatcgttg agtagcaaaa caacttctgg
     9121 cattttgcaa cttcacacga aaaggagtcg ttgatgagtt ggcttatcgt tattttgatc
     9181 gctgttgttg taaacatcat tgtcctcgcc acttgcggcg gaggcggagg cttgatgctg
     9241 ctggttgcgc tcaatggatt ttctgaatcg gctgcaatgc cgtttttggc gggctatggt
     9301 ctgttggtgc tcgccatcag ttttggaatt gccacaggcg caacctggat atatgtgaaa
     9361 ccccgcagca cagcctctgg aattggattt tggcaggtgg caggtctcaa cgcggggatt
     9421 atcgtcctgt tgattttggg ggttgttgcg ttcatggcga tcagggcact ttgatactgc
     9481 gatgatcatt gttgtaatgg gtgtggcagg ttcaggcaag accaccgtgg gcgcgttgct
     9541 cgcgctcgaa cttggctggg atttttacga cggcgacgat tttcattccg atgccaatcg
     9601 cgcaaaaatg tcacagcgca tcccgctcaa cgatgaagac cgctccacgt ggctggctgc
     9661 ccttcgagac ctgatcaacc aaaatttaag tgagaatcgt tccatcgtgc tggcatgttc
     9721 tgcattgaaa gagtcgtatc ggcacatgct caaggtcaat gagcaggttc attttgtcca
     9781 cttgcaggga agttatcaac agatcgaaga acggctgcgc gggcgcacgg ggcattttat
     9841 gtctgcggag atgcttgcca gtcaattcga tatccttgaa acgccagaag acgccttgtc
     9901 catcaatatt tcaaacacgc cgcaggaaat tgtggcgatc atttgcaaag gattgaatcc
     9961 atgaaactcc catcacattt atatctcgat gaaaagaaca tcccgtatga agcgttgagc
    10021 ttttcgccag aaacagaaaa gggcgcagcc aatgttgcac acgccctcgg tttttccgaa
    10081 cggcaggcag tgaagacttt gatttttcaa gtggacactg gtgagcgtgt actcgtgatg
    10141 ctgggcggtg atcaaaacgc agtctcagga aatttgaaga aagccattgg ttcgcggaat
    10201 attcaaatgg ctgcgcctga cgtcgtgaag gagaccactg gatacgtcat cggttcgatc
    10261 cccgcctttc actggcagcc agaaaatttc agatctttcc ttgaagcgag tctccttaat
    10321 gaacccatcc tcggcacggg tggtggtcaa tggggtgaag agatcatgat aacgcctgaa
    10381 aacctggtca aggccagcaa tgccatcgtc gtcaacctga cagaccgaga aaagcctgta
    10441 tttttggaat cggtttagat aggactgcag ttttcgaaat tacaagcaca ggtgatttgt
    10501 gaagatgatc ggaaagtcat ttcctgcgaa aaagaccatc gtactggtga ttctgtcagt
    10561 agcgggcgga cttcttgcag ttgtaaattt tttcctgctt tcgaaagata tgctccgtgg
    10621 agattattca gcgaaagata tttttccaca gccgatgtat gggttcttcc gtgtgcctga
    10681 caggccgaac accatcgcac agaacggggt aaacaggctg gctggggatt acgctcaagt
    10741 ttattttccg tcgctggaat tctcttccct gacccaaaat tacgaaacgg gatatttaga
    10801 cccgtggaaa agaccttcac gctatgcgcc gttcattcat tacgtgtgcg cactttcctt
    10861 ctgcaaattg gattatgggt atgcaagcct tttgcacatg cttactcaaa tggttctgtt
    10921 ctatgcgttt tttattgccg cattcaagat tttggagatc gaatcagacc tttgggttgg
    10981 cctgctgata accaccctgc ttctgttcgt tacacctgcg gggttatcct ggttcgagcg
    11041 cggacaattt tcactgtatg ttgccctggc atatttattg ctgatgctgg ggttgatcaa
    11101 aaacaaacct gtattcgtgt ttatctcggc gcttttcgct tatgtcaaat ggacatcttt
    11161 tccatttctt tttgtggtgt tgttcgtaca ctggatgagt gcaaaaaaca caaaagagag
    11221 agcccaatac acacaatcgg cgttgatcta tttgctggtg attcttgtgc tttcgcttgc
    11281 gttccgaagt cgttttattc atttccttga gggtatttac aaacaggaag tcaatgtgga
    11341 ggcggtggga attgcactgg cacagttact gccgacaagc cttgtaaaag gcatgccgat
    11401 cctcctcatc ctgctggggt atttatcttt gcgaaaaaac aacatggatt ttgagcgcgc
    11461 cgccccattt ctgatcgggt cgggaatttt gatgctgacc taccccaccg ttgcattcga
    11521 atacaacata ccgaatctat tctgtttcat cccactgatc ttttactggg cgagaaatcc
    11581 caagttaatg aaccacgctg caaagtatgt tttttttatc ttcatttctc tggcttcctg
    11641 cacggcattt cttggctcaa cggtaggcgc aaaaacaatt ttattcggat acatggccgt
    11701 ggcgggcttt tttctaattc tgccattcat tgacaacaaa acgttcggaa acgcgctgaa
    11761 aggatgattt accaatggca aatccgcaaa ttcgaataca gaccgagttg ggggatatga
    11821 ttgtggagct ttaccctgca catgccccta taaccgttga gaatttttta cgctacgttg
    11881 acgagaaccg ctttgagggg gccacatttt atcgagttgt acatttggaa aatcaaccca
    11941 ataatcaagt caaaatcgag gtaattcaag gcggcctcaa ggatgaagat catccacaaa
    12001 gcctgccgaa tattgcacat gaaaccacaa cccagacggg aattctgcac agggatggcg
    12061 tcatttcaat ggcacggaat gagccaggca ctgcatcagc ggatttcttc atctgcaccg
    12121 gcgatcaacc tgagttggat ttcggaggca ggcgcaaccc agatggtcaa ggttttgcgg
    12181 catttggaaa agtcattgcg gggatggaaa cagcacgaag gatccaagcg ctgccagaag
    12241 aaaatcagta tctgaaaacg gagatcgcgt tcaaaataca gcgtgcccaa ccctgacggt
    12301 catatttttt accagcgcct cgcatggtta aaacaaaaaa cttgccacgt attctttcgt
    12361 ggcaagtttt tttctagcga tacgtcaacc agttcaggtc tctcagcttc cctgcaatgt
    12421 cttcgcgcag gtcatattca cccatacgcc attcccagtt accgccaagc ttactcggga
    12481 agttcatgcg ggcttcactg ccaagattaa gcagatcctg catcggggta atggcaaaga
    12541 cggcaaccga tttccacaca ccacgaatca ggtcccaggc aaagtcattg ccatccacgc
    12601 tgaggtagcg ttgtgcgaat tcacgttcgt gaggcgcagc atgttcgaac caaccgcggg
    12661 cggtgtcgtt atcatgtgtg cctgtgtagg ctacgctatt gggaacatag ttatgcggca
    12721 ggaagggatt ttcggggcct gaaaaggcga attgtaaaac cttcataccg ggcaaatcaa
    12781 aagcggccaa taactcgatc acatccggtg tgacaagacc aaggtcctcg gcaatgatag
    12841 gcaggcctgt gccatgcgtg attaggccat caccaaggaa tttatccaca gcgcggaaca
    12901 ggtcttttcc ggggccagtg acccaacgac caaattcggc tgttgtgtga tccgcaggga
    12961 tctcataata cccagcaaaa ccacgaaagt ggtcaaaacg caaaatgtcg aagatctgca
    13021 aagaggaacg gacacgtgaa agccaccagg catagccttc tttcttgtga acatcccatt
    13081 tgtaaagcgg attaccccac aactgccccg ttgcagagaa atcgtcaggc ggtacacctg
    13141 ccacaacggt tgggttgcct tcacggtcta aaaagaacag atcgggatgc gaccagacat
    13201 cagaagaatc ataggcaaca aagatgggaa tatcgccgat gatctgaatt ccgcgttcgt
    13261 tggcataagc gcgtaacttg ttccactggc ggaagaaaat gaattgataa aaagcaaaac
    13321 gcatgacact ttcgccaagt tccttctgcg ctttgctcaa ggcggctttc tttctcttac
    13381 gaagggcttc gggccaacca ttccacgcac cgccgccgtt ggcttccttc aatgccatga
    13441 aaagagtgta atcatcgagc caggaggcat tttcagcgca gaaatagtca aaggcttggc
    13501 gtaaatgctc agggttggat tggtagcggg aaaatgcctt ttgcaaaaga tccagcttcc
    13561 ttgggatgaa caggccaaag tccacgtgtg aagcgggcag gtctttcatc ccatcaaggt
    13621 cttcctgagt caataatcca tctgcaaaaa gttcgtcagg gctgatcaaa tatgggtttc
    13681 ccgcaaatgc ggaaaaagcc tgataaggcg aatcgccata ccctgtcggc ccgagcggaa
    13741 gtacctgcca gagtttgcag cctgtcgatg caagccagtc gataaagcgg aaggactgcg
    13801 ggccgaggtc accgatgccg tatggagatg gcaggctgga tggatgaaga agaattccag
    13861 aagaacgttt gaaggtcatg atttaatgag ggagagatat agattgagat attgtcttgc
    13921 tgaattttcc caggagaaat cctgagccat gcctgagcgt tgaagtgatt gccattgctc
    13981 gcggtttgca aaaactttca gcgcattctt gaccgccgcc accaaggaca ggtgatgcgc
    14041 tttttcaaaa acaaagcctg ttacaccgtg acgaacagta tcattcaacc cgcctgccgc
    14101 acggacaatg ggcacacagc cgtaacgcat tgcgatcatt tgcgagaggc cgcaaggctc
    14161 ataacgcgac ggcatgagga acatgtcgga gcctgcataa atctggcgcg caaggcctgc
    14221 atcgtaacgg gtctcaactt tgaccttttc agggaaaagc ttttgcagtt tcattgcggc
    14281 ttcttcgagt tttgggtcgc ctgtgccaag gataaccgcc tgccagttca aacgcttcat
    14341 tgtgttgagc gcattaaagg caaggtcaac acctttttgg acatccatcc gcgaaacgat
    14401 ggcgaaaagc ggaatctcag gatcctgcgg aagcccaagc cgtgcttgta agaggccttt
    14461 gtttgcggca cgtttttcga gggtttcaag tgcaaaattg acaccaatgg cagggtcatc
    14521 agcggggttg aacgagtcgg tatcgaggcc gttcaaaatg ccgctcaagg tttcgcggcg
    14581 cgattgcaga aattcattca agccgcagcc gtattcctcg gtcagtactt ctttggcata
    14641 ggttggcgaa acgcccacaa tggcatctga agaccaaagc cccaaaggca ggggcatcac
    14701 acgcgcccaa tcaggcagat cggtttgggc gagtttcagg ccatagcttt ccaaaatacc
    14761 gctgacatct ggacccatga atggaagatt gtgcaacgtc aataccgaag cgacatggcg
    14821 ggcaccttct tcccaacgtt tggtcaggga ataatacagg ctcagagccg tatgccaatc
    14881 attgccatga acaacgtcag ggaaccagtt gatctgccgc gataattcca gtgcggcaat
    14941 ggaataaaaa gaatatttct cggcgtccag tttggcatcc aaagagtaga ccgaaccatt
    15001 ggcacgaatg gcatcaccat taatgaagta gacaggcatt cccttcaggg atgtttcaaa
    15061 cgcatccacc tggacctcgg aattgcctct cggaatcgaa aaaatgccta tcggcctgag
    15121 attttcggct ttgatggcag gatgatacgg caacacaagc cgcacatcca gtgtaatttc
    15181 atcgttggaa agggcgcgca aagcgcgcgg cagagaccct gccacgtcac ccaacccacc
    15241 tactttgata aacggttcgg cttccgccgc aagaaaaagg acattaatgg tttttggcat
    15301 ggcggttatt ttaccttaca taaagaccct aaaggctaaa tacctttagg gtcttccagg
    15361 ggtgcctgaa gggtttcgaa ccctcaacat ccacatccac agtgtggcgc tctgccgttg
    15421 agctacaggc accatattta gagcggcgaa attttaccac aattcaccgc agattcaaca
    15481 ccgccaatgt ctcgccgtca tacatctccc cgggctcttc aaaaccgagg ctggcataca
    15541 acttccttgc ggattcgttc tctggctcat aactgatgcc cacattcctg atcctttcat
    15601 cggcacgaag acgatccagg gctttcgaca taatatcgcg gccataccct tttccttgaa
    15661 acttctcatc caccattaaa cgggcaataa aagcctgaaa gcgcgagtgg gtaaaattca
    15721 aacaatacat caagaaacca acaggctctt cacctgcata cgcaccaaaa gggaacatct
    15781 cccaatgccc ttcatcctca aaaccaaatt gagcttcagc aatggaatac agattagaag
    15841 ccacaaaatg tgtctgatct tctcgtacct tcaaactgat cagtgcccgc cagttttcag
    15901 cagaaacggg atgaagagaa atcgtcacgt gatcatccaa tcatttttcg aatctcggaa
    15961 accaccgctt cgcgctgcac cgtgacctgc tcgccagtca tcaggttttt tacggcggcc
    16021 tgaccattcg ccgcttcgtc tggaccaacg gtgacagcga gcttgatctt cattttgtcg
    16081 gcaaatttga attgtttttg cagcttcgca ggttcgggga ataccatggc attgatccca
    16141 gcctcacgga gttgcgcggc caactcaaac gatttcaacc acatggcttc atcaaaaacc
    16201 gtcaccaaca ctgcggcagg cgttggggca aattcaggca caagaccagc ctcttgtaaa
    16261 acaatgcccg ccaccacatc gcccatggca aaaccaacac ctgaaagcgg ctgtccgcca
    16321 acatcagcca gaagattatc gtaacgccct ccgcccagga tggcacgctt caatgaaccg
    16381 ctggtttcaa aagcctcaaa aaccgtcccc gtataataca agagaccacg catgatgttc
    16441 gggtcaaatt tgacatactc gctgacgccc aaggctttca atgcagaaaa caggcgaacc
    16501 aactcctcgc tatttttcca cagatcgaaa tttccaagga tgcccttcaa cccatcaagt
    16561 tgggtttgcg tcaggcccag ttcaagtgca tacgaatccc atttggcagg atccattttt
    16621 gtacgacggt ctaccaggtt ggaaacatca aggcgccttt cgggagccag accaagggcg
    16681 tcgaattcag aatccatgag gcgccgatta tttacataaa tggcagcccg ctccggggtg
    16741 agtccgaccg aacgcaggaa tgtcgccgca acagcaataa gttccgcatc cgcttcggga
    16801 gaattgactc ccagcatgtc aatattccac tgaaagaact cacgtgtacg gcctttctgc
    16861 ggctgttcgt aacgccaaca tgggccgaac gaccaccaac gcacagggaa ggttaactca
    16921 ccctgcttcg ctgcaatcat acgcgccagg ctgggcgtga gttcggggcg cagagtcaca
    16981 aagtcaccgc cgcggtcggt gaaagtgaag gactgcttct tcaccagctc ctcccccgat
    17041 tttgccgcgt atagatcgat cgtctcaata aagggtgcgt cccattcttg atacccaaat
    17101 ttctgagaag ccatgcgaac tttttcataa atgaaatttc gcagcgccat ttgctcagga
    17161 tagaattctc ttgtgccttt tacaggttgt attttgctcg ccattttgac tccagtgtgc
    17221 aaattttatc atgggtttca aatgccccca aaattcagcg attgaatttt gattttgttt
    17281 tggtataatc tcaaaacgtt tattgcagtg cggactttgg ttgtctgcac taacacgatt
    17341 aaagttgtca tacacaagga gataaaatga aaattgatta tcaagaaccc agcaaagacc
    17401 tgctgatgcg cattgacatt catgaaaaat atggctcggc caatatcgac gtttggacga
    17461 acgacctgct caaacctcag gctggcatga aaattctgga tgtgggctgc ggtgctggca
    17521 agctctgctt ccttttcaat gaatttacaa aaggcggcgc gcaaattaca ggcggcgact
    17581 tttcaaatga attactcgat aaggcccgcg agaagaacaa aaccgtcggc gccaatattg
    17641 attttcaatt tttggatttc aatcaacgtt ttaactttgc cgacaatacc tttgatttgt
    17701 gtaccagcgc atttgccatc tattacgcct ccgatcttga ctttacattt ggtgaggccc
    17761 accgagtgct gaatccaggc ggacggcttt tcgtctccgg gccattgcct gagaacaagc
    17821 aaatgttcta tgacatcatc aaggaagcca caggcgctcc catccccccc atgcccgggt
    17881 catcgcgctt caaaggtgat attttcaaca ccattgacaa gatatttgcc aaaacagaac
    17941 tgcacaagtt cgaaaaccac ctcaccttcc ccgaagtagc cccgttcatt gattacgtcc
    18001 gcgcttcttt gggcgaagac cgcagactgt ggacatccat gtttaacggc aaggatgagt
    18061 acgaagccct gatcggcaag atcgaggctg tggctacacg ctggtttgag cgagatggaa
    18121 aactcgtcat gaccaaggtt gtcggcggaa ttctcgcgac gaaataaacc aaatcacgac
    18181 ccgaagataa ttgacgaaag ccagttgctt cgggtccccc acattcacca tgaatttttt
    18241 cggaaaacga gttctctttt taggcgccca ccccgacgat attgagttgg ggtgtggagc
    18301 gctgttgcac aagatcgcaa agaagacaga tgtgttgtgt gttacactct ctgacaatca
    18361 gaaaaacccc gacctgcaaa aagtaaaaag cgaacatttt caatccatgc aagtgttgga
    18421 tgtgcctgaa gaaaagatcg tactgggacc attcaccaca cgggttttcc ctgatgcgcg
    18481 gcaggaaatt ttggagtact ttctgaaact ccgcaaggat ttcaaccccg acctgatctt
    18541 tacccatacc cgacaggatg tacaccaaga ccacaacacc atgaccgatg aagccctgcg
    18601 cgcctttcgt ggaatcactg ttttgggttt cgatgttgtg cggtcctctt acggcttttt
    18661 ccccagcttt ctggtggagg tttctgaaga aaatgcggct gcaaagatcg aagcgctttc
    18721 aaaatacgag acctaccgtg accgttacta tttcaacgca gaattgaccc gttccatcat
    18781 ggttcggcac ggagccctgg cagagcggcc actggcagaa ggatttgaca ttctgaggat
    18841 tgtcggcaaa ttcggggagt gacgacatat cttgtttgac atcaattctt caaaacccta
    18901 taatcaggca aaatacccct caaccgccgc aaggagaaaa aatcatgcaa aagtacgagc
    18961 gggcagtttt catttcatat gcttggggcg atgaacgcga agagatcgtc aaccaacttg
    19021 acaaggctct gcaaaagcga ggggtcaaga ttatccgcga caaacgcgaa ctggaataca
    19081 aggggtccat ttctgaattc atggagcgga tcggtcgcgg cgactgtgtc attgtcgtca
    19141 tcagcgataa atacctgcgg tcaccgaatt gcatgtttga gctgatcgaa attgctgaaa
    19201 ataaaaagtt tcgagagcgc gtttttccga tcattttgtc agatgccaat atctacgacc
    19261 ctgtcaaaag agtggaatat gtcgatttct gggaaaacaa aagggatgag cttgccaggg
    19321 ccatccaaaa acttgacccg gcaaacctgc agggcattcg cgaagacatg gatctgtacg
    19381 accgtatccg cgacatgatt tccgggctgg caagcaccct aaaagacatg aatgccctct
    19441 caccacaaac acaccgcgac actgattttg aagatctgta taacgccatt gagcaaaggt
    19501 tggggcagcc ccagtccgcc cgccccaaga aaccgagcaa acccgcaaaa atagaaaaag
    19561 acgaaactgg cacaagaaat atagaaatag gcggcagtgc aagcggcaat gtgttcattg
    19621 gcgataacaa caagatcgaa aaataatcag tcagaagttc cgtcttttta gaggtcagca
    19681 tggcaaatgt ttcaattggc ggcaatgtaa cagacagtgt tttggttttc ggaaacaaca
    19741 actacgttgt caagatcggg gatgtgaacg gcggcgtggt caatatcgtc aagccttccg
    19801 acaaacccaa ggtctccgca cgccctgccc cggtcatgat aaagccgcgc gcctttccct
    19861 ctttaatgga ccgtgtcgat gaagctgaaa cggttaaaaa ggcggcagag ctggcagtcc
    19921 ctgtttctgt ttggggaaaa caaggcatcg gaaagacctc tttcatccgc catttggcac
    19981 acatccttcc cacaaacaaa tatccaaatg gtgtagtcta tctgaatgtt tccagccttg
    20041 ggcatgaaga cctgcttcaa gctttattcg atgcattttt tgaaagcgac aacacttaca
    20101 agccaaccac agctgacatc cttcatgggc tacagaagat ctccgcactt atttttcttg
    20161 atgatctgca aattggccgt gatgaaacca tttccatcct gaacgcagca cccagttcca
    20221 cttttatcct gatctccagt gaacgcagtt tctgggggga gggcgaagcg gttcaattgc
    20281 aggggttgcc tgttggggat tcggtacggc tgttcgaaaa agaattggca cgtccgcttg
    20341 aaacggaaga aaaaatcaca gtgccaaaaa tctgcaaaat gctgcagggt cacccgctca
    20401 gcatcctgca atcggcttca ctggcgcgtg aaagcggtaa accccttgaa gagatactga
    20461 acggattgac caatgaaaag aaggatgaca agtccatggc atacatgggg ttggccagcc
    20521 ttacagactc ggaaaaacag gttattgccc tgctggcagc cgctggcgga accatcgttt
    20581 tggcagagca tatcaagggc atcttcaaag gggacagccg tcagacagat attcaaaagc
    20641 tgatccagct cgggtttgta caggcacaca gcccaaagtt cagtgtgacc gaggcattcg
    20701 cttcttccct tgccgcgacc tgggatctat cttcatggca tgatatcctg ttgaagtatt
    20761 cgatcaagtg gttgagtcaa cagccagcat cggcattggt ggatgaatcc accaacctgc
    20821 tgatccacac catcaaaaca gcgggggaaa gaaaaaaatg gagtgaagtg atccagcttg
    20881 ggcgggcgct cgaaaagttt ctggtctatt acaaacgctg gcaggcatgg gccgatattt
    20941 tgaagttgat catcgaggcc gccaaagcct tgagcgacag caaaacacag gcctgggccc
    21001 tgcatcaact cggaacccgc gccctgtatc ttggtcagac cagcgaagca aactcgctcc
    21061 tgacacaggc acaaaccatc cgcaaagcca ttggagacaa agccggttta cgggttacac
    21121 aacataattt gaaaaccatc aaaggtggat cggggtgtgc aaaattcgtt tcctattttg
    21181 tcggtgctgt tgttggcgcc accgtactga cgggaatcgc ctggctcgcg atcaatgttc
    21241 tattttctcc accgatcccc atcgctttac caactgctac aaacacacca accttgcgac
    21301 cgtcgtcgac cattccccca tcaaatacgc ctcgaccatc agaaacgccg acagttcgac
    21361 cgtcgaggac gccaagaccc acagacactc caatgccagt gctgttgtac gattttgttg
    21421 aaagcgcgca cgaggtttat tggtaccaga ccacaagtaa ttctgatggt gagtttactc
    21481 aagacctgcc atttttcttc gagccggttg ttccagatgt tgaatcgtac ctccctggaa
    21541 gtgacagcgc ttacgttggt tggtctttcg atacacaatt gaccaatggc aacagatatg
    21601 aaaaagtcct gctcacctac ccgctttacg agcatttcaa ggtcttcgga gattacacag
    21661 taaagatcgg cagacctgcc ccggaagcat acctcgaatt gaccgccggg tttaaggaca
    21721 ttgttattga accggtggag ggtgttatgt tcagggtgta cgtgaatggc aaggtgtata
    21781 ttgaagagcc atattacttt ggcagcgaac cgatacagac gggaagtcga atccgactgg
    21841 ctgcaaacac ctatgaattc aggctcgaag tggaatctct ggatccatct ccgtacgatt
    21901 tcgccacttg ggcggttgta aggctttggg acaaagaacc gtgagattat ttacttggcg
    21961 ctgacaaaaa accccgcagg tgcttttcgc ggtgacgcaa aatgtatttt agatacccaa
    22021 acaataaaaa aggacgttct cggttgagag cgtcctttta tgatcttacg gcctattctt
    22081 tcggcacgcc aatgaaaata attccatccc gcacctgcac aggataagca gggatatcca
    22141 ccacagcagg catggacatg accttccctg tccgcacatc aaactcggca ccatgccgcg
    22201 gacagacaat attatggtct tcgatcaccc catctcccag cgggccatca tcatgggtac
    22261 aaatatcacc tatcgcgaaa aactggccag ctatgttgaa aataacgatc ggcttgtcac
    22321 ccaaatcaac gaacaggcgc tcgccattgg gcaattcgga ttcgggtgcg atctcaagaa
    22381 attcgacttt ggattcatca agggcggtgt aattaaacat aaattccttt tattnnnnnn
    22441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccttt tattcaacca aagagtcgct
    22561 tgcctcacca agaccataca cacctgcttt caaaaccttc aaagagagca aggcgcattt
    22621 caagcgcaca gggcccagat caataccaag catttcaagg acagaatcct tgtttaattt
    22681 tttcacttct tccagcggtt tgccgatgat ggtttccatc aacaaatctg cggaggcttg
    22741 agaaatcgcg cagccatgac cgtcaaaacg ggcatcggtc accaggccgc ctgcgtcaac
    22801 tcgcaaatcg atctggatat gatctccgca aaggggattg ttatctgcaa aagaaatgtc
    22861 atgcggatcg agctttcccc gatacgaagg gtgtttatag tgctcaataa ttacttcacg
    22921 gtagagatcg tccatctgtg ctccaaacag gcaaacctga aaacggtcta cccaaacatt
    22981 tccttcactt tgtagatccc gttcaccagc aaatccactt cttcttttgt gctgtacaaa
    23041 tagaatgaag cacgactcgt ggcagggatg ccgaattttt catgcaaagg ctgggcgcaa
    23101 tgatgcccag cacggacggc aattccatcc tgatcaagga tctgcgccac atcatgcgga
    23161 tgcacaccct caagagtaaa cgccgccacg ccgccttttt tatcggcaga tgggccaaat
    23221 agtttcacac cggggatctc ttccagccgt tcaagtgcat actcggtgat ttcatgctcg
    23281 tgcgccgcaa ttgcatccat gccaactttt gaaaggtagt ctacggctgc accaaagcca
    23341 atggcttctg cgatggcggg agtgccagcc tcaaatttat gcggcagggt atttgcgcgg
    23401 aacgagcgca gtttcacttc cttgatcata tcgccgccgc ctaaaaatgg gggcatcgcc
    23461 tccagcaaag cggtcttgcc atacaatgcg ccaatgcctg tcggcccaca catcttgtgc
    23521 gcggaaaaag caaggaaatc tgcatcaaga gcctgcacat ccactgtcaa atgcggcaca
    23581 gactgtgcag catccaccaa tgtgattgca cccactgcat gagccaaacg aatcatctct
    23641 gcggctgggt tgatcgtacc caaaacgttg gacatgtgcg taaacgaaac cagcttcggg
    23701 cgacggtcaa gaagcgtttt atacacctca agatcgagta caccgtcctc cgtcacagga
    23761 ataaattcca actcgatacc gcgctccgct tgcagcatgt gccacggtac aaggttgctg
    23821 tggtgttcca tctcggtcag gatgacaagg tcaccctgct tcaagttggc gcgcgcccaa
    23881 ctatacgcca ccagattgat cgactcagtg gtgttgcggg tataaataac ttcgcgtgcc
    23941 gatgcggcat tgataaattt cgcgatccgc tcacgcgcac cttcgtacat tgcggtggct
    24001 tcttcagcca aagcatggac accacgatga atgttcgcat tcgaaaggcg gtaatactcg
    24061 ttcattgcct cgatcacagc caacggtttc tgcgaagtgg cggttgagtc caaatatgtc
    24121 aaaccgaccc ctgcgcgtat ttcacgctca aggatcggaa agtcttttct tacctgggat
    24181 acatttagct tcatgatcgt ttcatcaact tttagatttg gcgactcgct ggcctaatat
    24241 tctggaatga aatccttcac cggcggcttc tttcccggta cggggcgcat gtgctcggaa
    24301 ttcaacggga accacaaaat acggtcagga accatccaac cgtccgtgag gtacacattt
    24361 gaacggaact gcacggccac cattttgttg tccacctgca caacgatccc cttcacccac
    24421 ccacgaatcc gttcgcgatt cttctcgtgg tcgcaaaaca cttccacgct tccacccact
    24481 tcgtaatcat gctcggcacc gggtgccttc ataaaactaa actgatttgt cctgctcgga
    24541 cgggcaacag ggcggcggcg ttccaccatt tgatttacaa cacgtttgat caccggtgta
    24601 acggctggtt tcaccgctgt gggatggttc gaggtttgct gttcaacttc ctttttaggc
    24661 gcagcagtta caaccttgga agcctgcatc ggtttctttt ttacttcagt cttgatcact
    24721 tttgttttta cggtcgtttt cgttttgact gccacccgtt ttttcgaaac tgtcctggct
    24781 gtcttggcct tcgcactggg ctttaccata cgcttgctgt ttgatttcac tgccataact
    24841 cacatcctat tcttttgaat ggaagagacg ccctccgcct ctcacttaac tgacaattgc
    24901 cccggcaaaa tcggcattcc caccgccgat aattttgccc gaaaacctga caagccccaa
    24961 agggacctgt caggtttcgt tagattacgc tgatagcttg tcctgaatcg cctgctggaa
    25021 cctttcacgc actccctcaa atggaatacg ctgcatgatc gggtcaaaaa agccctcaac
    25081 caccaatcgt tccgcctcac gctgcgggat accacgggat ttcaaataga agagcggttc
    25141 ctgctccaat ttgccgacag tggccccatg cgtacagcga acatcatcag caaggatttc
    25201 caaacctggg atcgaatcag cgcgcgcctt gtcatcaagg acaagattac ggttggcctg
    25261 atacccatct gtcttttgtg cgccgggggc cacatagatc atgccctgcc acacagagcg
    25321 gcttttgcct ttcaaagcac ctttgaacag taagtcactg gtggtgtgcg gagcaagatg
    25381 attctgctgg gtatcatggt cgagatgctg ggttccatct gtaaaataaa aacccgacat
    25441 acggcccacg gaaccttccc cggcaaggtc aaggtcagag aagtttttgg tcagacggga
    25501 acccactgcg ccaaaaatcc aatcgaggtt gccgccgcgc tccacgcggg cgcgctcgtg
    25561 cgaaaaattc cacacatggc gaccccacga ctgcaattca acaaaacgaa ggttggcatc
    25621 cttgccaacg taaatttcga caacacctgc atgcatggca tgccccacct catcaggtga
    25681 agccgcttca tggacatagg tcaccgaggc gccatcttcc acataaacaa gaagatgcga
    25741 gaaatgagcc agttcagagc ccgggcccca caagacagaa tgcaaaggct gttccacctg
    25801 tacattcctg ggaacataca gcaaaacacc attttgagcc aaagcagaag caagcgcggc
    25861 gaatttgcca tcatcaggtt taacgatctg cccgatcaac ttcgccagca aatcggcatg
    25921 atttttctcg gcagtgcgga aatcagtgaa gaccacacct tttttcacca gagactcgtc
    25981 caagtccact ttggagccgc cggggagcag ggtgatctgt ccgccatgct gttcaccggt
    26041 caacggctgg agcagatact cgggcacggc aggcaggtca tcaaaggctc cgatcttcgg
    26101 cagcttgaaa tcagaagctg ggagcaagcg caggtcggtg cggcgccagg cttcgtctgt
    26161 gtttacgggg agagaatgct tgttgaatgc ttcccaagcc gaggcgcgga aagaagcgag
    26221 gctgcctgca gcggaaacat ctgcctgagt aaaagcaaat tcctttgcgg cagtatctgc
    26281 gcgtcgttgt cgtgttacag taacttttgg tttctcttgc atgttcaatt ccgtttcaaa
    26341 gttggaaagt tcactggccg gaagattcac ctgccagcaa tttaactttc taaccgatag
    26401 acccttccat ttgtagttga atcaaacggt tcatttcgac agcgtattcc atcggcaact
    26461 ctttgacgag cggctcgatg aaaccagaaa caaccatggt tgtggcttct tcttcgctca
    26521 agccgcgaga catgaggtag aaaagctgct cctcgcccac tttgctgaca gaggcttcgt
    26581 ggccgatggt gacgtcatct tcatcaattt cgatgtaggg ataagtatca gaacgcgatt
    26641 tggggtcgag caacaacgca tcgcaaacca cattggattt gacacccttg gcacctttat
    26701 acactttgag caagccgcga taggaagcac gtccgccatt cttggaaatg gatttggaag
    26761 tgatcttgct ggtggtgttc ggcgcgaagt gaaccatctt tgaacccgca tcctgaattt
    26821 ggcccgtgcc cgcaaacgcc atcgaaagca tttcgccgtg cgcgcctggt tccatcagat
    26881 agcaggaagg atatttcatg gtgactttgc tgccgaggtt ggcgtccacc cattcgtggg
    26941 tgccattttc aaaaaccttg gcacgctggg tcacaaggtt gtacacgttc gttgaccagt
    27001 tttgaatggt ggagtaacgg gaacgcgcac cctttttgac aataatctcg atcacaccag
    27061 agtggaatga gtcagtcgtg tattgagggg ctgtacaacc ttccacgtaa tgcacctgcg
    27121 cgccttcatc gacgatgatg agcgtgcgtt caaattgacc gacattggcg gtattcaagc
    27181 ggaaatacgc ctgcaaaggc agatccactt ttacgccttt gggtacgtaa acgaaagaac
    27241 cgcccgacca aacagcagag ttcaatgcgg caaatttatt atcttcaatc ggcacaaccg
    27301 tgccaaaata ttcacggaac aattcggggt gttggcgcaa cccatcttcg atggacagga
    27361 agatcacacc ctgtttctca aggtgttctt gaatcgagtg gtacaccatc tcagactcgt
    27421 attgcgcgcc aaggccggcc aaaaacttct gttcggcttc tggcacaccc agcttgtcaa
    27481 aggtgcgctt gatatcatcg ggaacatcgt cccatgattt ttcgcttttc tcggtgggct
    27541 tgacgtaata ataaatatta tcaagatcaa gcgagctgag ttcaggtccc caattgggca
    27601 tggggcgcgc tagaaaatgc tcaagcgcct tgagacgaaa ttcaagcatc cacttcggct
    27661 cgcccttcat gcgtgagatg ttttccacaa cctcacggct caaccctttt tcagacttaa
    27721 aggtgtagtt gtcatcgcgg tcgtgaaaac cgtatttgta ttcgccgatc tcatcgagga
    27781 ttttagtatc ttcagacatt cgattcacca ttttctggat gacgctccgc ttcacacgga
    27841 cctgtcacct gctactgata aacaaacaga ctctaccctt cggcaactgc gtcgtatttc
    27901 tcgcggaccc agtcatagcc ttgatcttca aggtgaagtg ccaggtctgg cccgccagat
    27961 tcaacgatgc ggccattcat catcacatgc acgaagtgcg gtttgatgta attcaaaagg
    28021 cgctgataat gtgtgatgac cagcacccca agctcaggtc cagaaagagc gttgacgcct
    28081 tcagaaacga tgcgcagggc atcaatatcc aaacctgagt cggtttcatc caaaactgca
    28141 aagcggggtt ttagggttgc catttgcaga atctcggcgc gtttcttttc accacctgaa
    28201 aaaccctcgt tgaggtaacg gcctgcaaaa ttatggtcca ttttgagcat atccatcttt
    28261 tccttcagca ttttgcggaa ttcggggatg ggcatgcctt tatcttcggg attttcagcg
    28321 cgacggcggg aattaattgc cgagcgcagg aagtttgcca cggttacacc gggaatggca
    28381 acagggtatt ggaaagcaag aaaaactccc gcacgggaac gttcatcagg ttccatttca
    28441 aggatatttt caccatcaag cagcacttcg ccgctggtga ctgtgtaatt ggggtgcccc
    28501 atcaatgtat aagccagtgt agatttacct gtaccgtttg gccccatgat tgcgtgagtt
    28561 tcgccctgtt taatcgttag tgaaagccct ttcaaaattt ctttgtcttc aatgcttacg
    28621 tgaaggtcct ttatttcgag ctgcgccatt cttttactcc tgaaataagc catttctggc
    28681 gtttttgatt aagttggcgg cattatagca gggttgaccg aaattgtcaa atatttatga
    28741 tatttttcta gtatattccc ttttggcaca ataaacaccc cgcaaatcaa ttcacctaac
    28801 caaatacaat ggcaag
//
LOCUS       DAOQLC010000050        28559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_526_length_28559_cov_22.221267, whole genome shotgun sequence.
ACCESSION   DAOQLC010000050 DAOQLC010000000
VERSION     DAOQLC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_28559_cov_22.221267"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PL000_10435"
     CDS             <1..1008
                     /locus_tag="PL000_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89362.1"
                     /translation="VDDFNLDIEKGEFVSFLGPSGCGKTTTLRMVAGFEIPTSGKIML
                     DGADITDKAPNQRNVGMIFQSYALFPNMTVAQNIGFGLRVRKEPEATIKQRVEEMVSL
                     INLEKHANKYPYQLSGGQQQRVSLARALAIHPQVLLLDEPLSALDAKIRISLRSEIRA
                     IQKKLGITAIFVTHDQEEALSISDRIVVMNTGVMEQVGTPFEIYNFPKTRFVANFVGS
                     LNTADAEVVDPANGLVSMDGVQFESAEGTEGRKKGDKVKIAIRPERLSFAADQKKANV
                     VDATIENITFLGSVVRIQVKIGNTKFNMDTFNNPFLELPALGSKEQVTCSKEAVLVLD
                     E"
     gene            1080..1895
                     /locus_tag="PL000_10440"
     CDS             1080..1895
                     /locus_tag="PL000_10440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC89363.1"
                     /translation="MNKVILVLSDALRYDVAKNNMGFLGHLVETKQASLHKIVGELPS
                     MSRPMYETIHTGMPSSEHGIVANSIVRLSAKPNVFQALKNAGKTTAAAAYYWYSELYN
                     RAPYHPVDDKETDDERLNIQHGRFYTEDEYPDVELFASAAHLIRKFSPDYLLLHPMGM
                     DYHGETFGADSKEYRNHAIYQDSKLAPLIMEWKERGYIVFVTGDHGINADGGHGGTTP
                     EQRTVPFFVIQPSGRGRGETGEVISHLQIAPTILKLLNVPIPESMKYPPLSLD"
     gene            complement(1881..2405)
                     /locus_tag="PL000_10445"
     CDS             complement(1881..2405)
                     /locus_tag="PL000_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HNC89364.1"
                     /translation="MRLQNKLIATLIAEGVEDLEYYVPLMRLQEEGAKVISAGLDMKS
                     VRGKNGLVITPDATIESLNADELFAIILPGGWAPDKIRRYDVVKNLVRKINDAEKIIG
                     IICHGGSIAISAGIIKKGQRATGSTGIKDDLVNAGAVWADEAAFREGNQVWGRVVADI
                     PDFNRELVKALVEG"
     gene            complement(2462..2881)
                     /locus_tag="PL000_10450"
     CDS             complement(2462..2881)
                     /locus_tag="PL000_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNC89365.1"
                     /translation="MKSYRKELWFNIPARRGFVNITAQVEACLEESGIQEGLILVNAM
                     HITASVFINDDESGLHQDYDKWLEKLAPHEPVSGYRHNDTGEDNADAHMKRQVMGREV
                     VVAVTEGKLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            3389..4051
                     /locus_tag="PL000_10455"
     CDS             3389..4051
                     /locus_tag="PL000_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89366.1"
                     /translation="MHPVTILLVDDHKLIRQGMRSLLETRKGYSVVGEAGDGQGGLEM
                     IERFSPDVAILDVMMPGLNGIEVTRLARQRGFKTKILVLSMFANAAYAVRALQGGALG
                     YVLKEADFSEILCAIDTVMDSRRYLSPEIAEEVLEMLLQTGNEKESDVLNLLSPRERE
                     ILQLVGEGKTNAVIADKLILSVRTVESHRFNAMKKLRINSHADLVKFAINAGLVTSEP
                     YI"
     gene            4074..5516
                     /locus_tag="PL000_10460"
     CDS             4074..5516
                     /locus_tag="PL000_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89367.1"
                     /translation="MTNQLPTILVIDDDPIVRQTIKTLLAGDDVRLAFAEDGPSGIET
                     AQKLLPDAILLDVMMPRMNGYDVCQALRADPSLSEVHIVMLTSLDDRDARLTGFMAGV
                     DDFITKPFDRLELHLRLKTITRINRYRRLMAERARFSWIVENSYEGYLLLSSDGTIQY
                     ANSSAQTFLHLPRECVGINITSHVVKHYSPYPTETWTAWLDTPAPCYLVQPETANARA
                     FWLLLEAIDMPVKSDNSRVVRVRDVTELLSTHHDMRKFHKAVTHKLRSPFSTLVMSMS
                     MLENRLQKLSESEIKGFVSLSLEAINFLAGDVNKVLDYIDTPLAMESNIPLEIRFLPE
                     MLKSIQNEQGLGNINLTIHPDLMTACLGITDYAFELVLGELIENSKKFHPDHAPQVDV
                     LVEPVEHENMVRVTVMDDGIRLTAEQLKWARMPYFQGEKFYTGETPGMGLGIPTITSI
                     IWQVGGEVVITNREDRDGLKVSLRIPLANE"
     gene            complement(5620..5901)
                     /locus_tag="PL000_10465"
     CDS             complement(5620..5901)
                     /locus_tag="PL000_10465"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HNC89368.1"
                     /translation="MLGQLLQLLEDKENGLSLSEISRALNAQPTAVLAMIAYLVQKGK
                     LLEIGPDGGHCASCGLVSDCNLLAARGKRYLRAELRDGKSIPGCVAPIE"
     gene            complement(5906..7591)
                     /gene="feoB"
                     /locus_tag="PL000_10470"
     CDS             complement(5906..7591)
                     /gene="feoB"
                     /locus_tag="PL000_10470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNC89369.1"
                     /translation="MHPTPQTIFRLDYGETLETEIASILAIFESMKFDTGKYSKRWLA
                     IKLLEKDSDILEHIQSMQGGREVIQFAVERAKQVESTLGEEIDLLTADRRYGFINGIV
                     RKSLQRPLIDRITLTDRIDDIVTHKWLGLPFFFAIMYVMFRLVIDVSSPFLDWTDTLI
                     NGPIAHLFSFLLDLVQAPAWMHSLLIDGIVAGVGGVLVFVPGLLILYFFLALLEDSGY
                     MSRAAFVMDRFMRVVGLHGKSFIPMILGFGCAVPAVYATRTIASRRDRILTALLVPLM
                     SCSARLPVYVVFGLAFFGSKAGTVIWAMYALGILVAMLAGMVFTRTLLKPDSTSAFVL
                     ELPPYRQPAMKSILIHMWENTREFVRKAGTVILGASVIMWLLLNLPLGVTNQKDSYFG
                     KVSGFIAPIFSPLGFGNWQTSGALVTGFAAKEIVVSTMSLVYTGSETTANAEPATFSE
                     EIISTGKGFAEATINSGKILVSILPGIDLIPEDEQTEDTVLSIALRNEFTPLSAAALL
                     VFVLLYVPCVATLGAIKHEFGNSWAVTSALYQTAIAWCTAFLVYQGGKILGFG"
     gene            complement(7591..8106)
                     /locus_tag="PL000_10475"
     CDS             complement(7591..8106)
                     /locus_tag="PL000_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNC89370.1"
                     /translation="MANLTIALAGNPNAGKTTIFNLLTGLHQHTGNWPGKTVEKKEGE
                     IEHNGMSINIVDLPGTYSLTAYSPEEIIARDFIIQEKPDVVINVIDATNLERNLYLTV
                     QLLELDVPVVLALNMTDALQKNGTKINTEHLSQMLGGIPILFTAANKGRGISDLMTKA
                     IESARKIKETA"
     gene            complement(8106..8351)
                     /locus_tag="PL000_10480"
     CDS             complement(8106..8351)
                     /locus_tag="PL000_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNC89371.1"
                     /translation="MPSSTQLHLLQPGQRATIVQINGAGALRRRFVEMGIVRGETILI
                     ERNAPLGDPVEYFIKGYHLALRREEAAQIEVNLDGGN"
     gene            complement(8368..9030)
                     /locus_tag="PL000_10485"
     CDS             complement(8368..9030)
                     /locus_tag="PL000_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNC89372.1"
                     /translation="MTIQYLSESTEMYLKAMLELSDREKVAVGRLAERLNVTPVSANE
                     MVRRLSEQGLMAHTPYKGVSLTAKGRKAACNVLRRQRLWECFLYEHLKIEWAKIYELA
                     CSLEHATAPEVTEALSVFLGDPKTCPRGNPIPAADGTFAPLEGTPLSMAPIGATVEVL
                     AVNATATDVLTYLFERGILPGKKITITEAAPLQGPLTLKAGGKETALGLSMAEFVIVK
                     TN"
     gene            complement(9222..9668)
                     /locus_tag="PL000_10490"
     CDS             complement(9222..9668)
                     /locus_tag="PL000_10490"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC89373.1"
                     /translation="MNRDFQIKNKTSREKMRELIEGMTDAELALPAVAGWTVAATFAH
                     VAFWDFRILHLLRLWRENEIVPSPYDVDAINEAQKPLCMALPPRTAAELALKAAQEID
                     SEIEAASDDLVKKIQDANVQFKFERNGHREYHIKEIEQVLAVHRAK"
     gene            complement(9671..10249)
                     /locus_tag="PL000_10495"
     CDS             complement(9671..10249)
                     /locus_tag="PL000_10495"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HNC89374.1"
                     /translation="MAIFKLVFWAAIIAEMVIRAPISRQQRKEAKSEQRVSAQEKTIL
                     GFLFVGMFLLPLIYSTTNWLDFANYSLPNWAGWLGVVLMGFALLIFWRSHKDLGLNWS
                     PSLEIRKEHTLITNGIFGYIRHPMYASQWVWVIAQPLLLQNWLAGFLNFLIFIPFYIL
                     RVRAEEQMMLDRFGDEYRTYMQKTGAIFPKGK"
     gene            complement(10261..10509)
                     /locus_tag="PL000_10500"
     CDS             complement(10261..10509)
                     /locus_tag="PL000_10500"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HNC89375.1"
                     /translation="MPDDFRTEEFKVDGEKLIAKIKELIHEGNIRRIIIKDKEGKTIM
                     EIPLTFGVVGVLLAPTLAAIGAVAALLTEATIVVEKSA"
     gene            complement(10522..11094)
                     /locus_tag="PL000_10505"
     CDS             complement(10522..11094)
                     /locus_tag="PL000_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC89376.1"
                     /translation="MTRNYLGQPPTAFQRRPHLTRDDEWIAALLKVAQVGHIASAYEN
                     QPFINPTTFWFDEENHQIVFHSNIAGRVRSNIEANPKVCLEVSELGKLLPSNVALEFS
                     LQYRSAVVFGEARLVSEPNEAKQLLYGLISKYFPKMTAGKEYREITDKELRATSVYAI
                     KIESWSGKENWEARADQSDEWPALDEKWFE"
     gene            complement(11084..11734)
                     /locus_tag="PL000_10510"
     CDS             complement(11084..11734)
                     /locus_tag="PL000_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89377.1"
                     /translation="MPAKSPKKETPISKDSMDLLIAEYNYIAATAAQSNEDRARVSSF
                     YLVAVGSLVAALFSTQFLNNVFNPDLINILFSILFLLLTLLGTTTILQLTRLRVAWFE
                     SLIAMDRIKEFIIARDKSLAGAFRWRIQNAPEFFKPNSVSFLQTVEVAILSALTFGTG
                     VFFFQKGIQYNCAPCNWAYSIALGLLAFFLQIWVYKKQLVLEKKRLQKEFSNNHDS"
     gene            complement(11807..13051)
                     /locus_tag="PL000_10515"
     CDS             complement(11807..13051)
                     /locus_tag="PL000_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC89378.1"
                     /translation="MNTAEFQKSLEKYADVIVKVGINLRKGQNLTISASITDYPLVRA
                     ITKSAYASGAKLVTVFYGDDEVFHTRMKMSSHKNLTEVPSWIVPAYTSIVKNGDAYIG
                     ITSDNPDLLADVDPEAIAIYRKTMGTKMRKLTQAVMRDAINWCVISYPAEDWAKKVFP
                     KTSTKQAQEKLWEAIFKTCRVDQRDPVAAWEKHIKDLSLRSAYLNKKRYAALHYKAPG
                     TDLTVGLPSQHKWVAARSKSQAGIDFTANLPTEEVFTMPHKDKVDGFVAASRPLVIAG
                     TTVEDFTLTFEKGRVVKVTAKKHEETLRKLIETDEGAARLGEVALVPNSSPISQSGIL
                     FYNTLFDENASSHIALGAAYPFTMKDGNKLSQGDFQKRGGNHSIIHTDFMIGSKDMDI
                     DGILANGKREPLMRKGEWAFKA"
     gene            complement(13094..14335)
                     /locus_tag="PL000_10520"
     CDS             complement(13094..14335)
                     /locus_tag="PL000_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC89379.1"
                     /translation="MDDQEFNQLLEKYAEVVVKVGLNLRNDQHLSIRGNVDDAPFIRK
                     VAETAYKAGARYVDVLWDDENIKRTRLQHADPETLTTLPDWLYNRYEEYSKRGDAELA
                     ILSSNPDLYNGIDSDRIAITRKTLYQKIVEPLRKYNNYANWCVVSTAAPAWAQKVFPN
                     LPEKEAQTKLWDAIFRACRIHTPDPVKAWEEHIQKLDKYKNYLNAKRFASLRYKAPGT
                     DLTIGLPEDHHWGGAQETFKNGITGTVNIPTEEVFTMPHKNKVDGLVTATLPLNNQGV
                     LMEEFSIRFENGRAVNFSAKKGDADLRKLIETDENAGRLGEVALVPNSSPISQSGILF
                     YNTLFDENASCHLALGSAYKSSMQGGVDMTDEEFAARGGNKSLIHVDFMIGSAKMDID
                     GIQADGTREPLMRNGEWAFSV"
     gene            complement(14400..15662)
                     /locus_tag="PL000_10525"
     CDS             complement(14400..15662)
                     /locus_tag="PL000_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC89380.1"
                     /translation="MPGKSHQELLKQYAEAIVKVGLNIRAGQRLIITLAATRGVPYQF
                     APLVHEVTKAAYAAGAKYVEVIWSDEEMMRLRAEHAPKDSFSEYPTWQVDAILKMIEN
                     GDALLSIGGSNPDLLGSLDPEKVGAMQRTHLQYYSKVSEKVTTNAINWCVVAAAGETW
                     AKKIFPDLDAQTAQQKLWDAIFETTRIDQPDPIAAWETHIVNLRKRAKYLQAKQYTGL
                     HYSAPGTDFTLGLPSGHQWISAQSMAQNGVMFTANMPTEEVFTLPDRNRADGIVTSTF
                     PLSYGGTLIEDFQVTFEKGRIVKVSARKGEAALQRLVDTDEGSHRLGEVALVPASSPI
                     AKRGHLFYNTLYDENASCHIAIGRAYRFTLTGGEELTDEEFVSAGGNVSLNHVDFMIG
                     SPKMDIDGIKKDGSREPVMRQGEWAIEA"
     gene            complement(15824..18085)
                     /locus_tag="PL000_10530"
     CDS             complement(15824..18085)
                     /locus_tag="PL000_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89381.1"
                     /translation="MNTSPTLFVLLLLLGALYLPIIFFAIQRRDDGHGISIWLAAFFA
                     LLAMVLNVAEAIEASQGGTLLFQEVQIYIAFTLIAILMLAFQNFMRYETWWVWVGVWT
                     LWMLGLALILTNALKLPDVLWSNGTLTLPRERLGPSWAILGWLIISIALVISISNAGK
                     QARQQLFRNRLNYWWPTVFLLFVNDVLLFSNIMIAGQPLRLMAAVLMVYVIGTHNVPD
                     LRQIFRRILAYVAIAVVIVGAYAAGYLLIEAVFGNSKNFNPLFAGAFIALLLTLIFAP
                     MLNLVSRAVNNWVKGDQYNPSHTIHKYSESISNILDMDRLASIAVGIILEAMQIERGF
                     LFLVDSDREADGHRTYRLRSARSPEERQMIAVELDENGIIASYFIREQKPLLQYDLDL
                     LPAFRAASPFERDWFNQLHTEVYIPIFAKKQWIGLLAFGPKLSRNRYTSEDLVTLSSH
                     ANQTAIALENARLVDNLMRLNNELRQARRALEKNNRDLERIDQAKSDFISIASHELRT
                     PLTVIKGYTEMLLEDTSLDPNYKQIMNSIHDGTIRLHEVMDSMFDIAQIDARSLKPHI
                     QPVEIGHLLQEVCIEQASSIKERKHSLSIDLPSLPHLKCDPNLMRKLFHHLIRNAVKF
                     TPNNGKITVTGHSIPAIIDLPNGGLEIVIADTGVGVDPNFREIIFTKFYQPGELGKHS
                     TSKTRFKGGGAGLGLALAKGIVEAHGGRIYVESPGYDEINFPGSQFHVILPLTKPEEG
                     EEQKTSTPVQFQV"
     gene            18141..21089
                     /locus_tag="PL000_10535"
     CDS             18141..21089
                     /locus_tag="PL000_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNC89382.1"
                     /translation="MLISRASRLKFNFSVDFFRPDGHVTFADLTAARAFAAQMSTPRT
                     DPVPGSDLYAFYLLDEAYHIILKHYFSRYTGVMGRAMGVLQSNLGSKYDLTLSKFIEE
                     FPPLSVFLGQTTAGVYLATKLPNFGDFGHGVRAAAVEEMLLINNSNNNPALSRYKVLI
                     DESVMNGSAYKELVQLLLDFFARQPGFGNGEFALGETLNELLLAPIKASPDSLEGQLQ
                     FIVQKWGHLLGEAFSAKLLRGMDFLREETIRLQTRQIDPSQKVETYVPSFVGSEYAEY
                     ERYSPDKDWMPRLVLLAKNSYVWLGQLSKKYQREISALDQIPDEELDLLASRGFTGLW
                     LIGLWERSRASQIIKQRMGQADAVASAYSLHSYDIASDLGGWNALNNLRSRAWARGIR
                     LSADMVPNHMGIDSTWVVEHPEWFLSTSNPPYPSYSFRSENLSNDLRVGIYLEDHYYD
                     KTDAAVVFQRRDLQTGDLRYIYHGNDGTSFPWNDTAQLDYTNPVVREAVIQVILHVAR
                     NFPVIRFDAAMTLAKKHVQRLWFPEPGAGGAIPSRSQYGMTKSEFDEKVPEEFWREVV
                     DRVAAEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMHMLRDEDNAKYRLAIKNTL
                     EFDPQILKRYVNFMNNPDEKTAVEQFGSGDKYFGICTVLSTLPGLPMFGHGQVEGFAE
                     KYGMEYRRAKLDETPDEGLLRGHEWRIFPLLHRRHLFADVEKFLLFDFYTSSGNVNED
                     VFAYSNRHNDERGLIVYHNRFANTSGWIKTSAAYIDKSSGRTLRKNLAEGLSLPRAGY
                     VIFKDCSSQLEYIRSCKDIWEKGMYIELGAYQCHAFVDFRFVGDVEWKTICDQLNGAG
                     VPSMQDKWKEMFAAKEETSVVEKTAKKKRVVKKAVTKKKETALAKAKKSPVKKKATVK
                     KPAKKKVAPKAKKAVETKPAAKAKKTVSTSKTTVKKTTKPKKGVESKKPVASVKTSKP
                     VRKKSSTKAK"
     gene            21454..21696
                     /locus_tag="PL000_10540"
     CDS             21454..21696
                     /locus_tag="PL000_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89383.1"
                     /translation="MDSGLIGKVEKAKRYAEDRHRFHFNQFDLDFHGDNSEHHVSYNN
                     GVFNCDCEFFITHKRCGHSMALEILLKDMITESVAS"
     gene            21822..22910
                     /locus_tag="PL000_10545"
     CDS             21822..22910
                     /locus_tag="PL000_10545"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC89384.1"
                     /translation="MKSQLSRRDFLKLGGLTLGGLAFTPFLPGLTDFDDGFVVRIATD
                     QMPVRSEPKDGSRIYQTWYRDDLVHVYEEVTAEEPKHNPVWYRVWGGYLHRGRLHRVK
                     TVYQVPMSSVPEGKRVLTEASVPYTTPYRFTKARGWEALNPPIYYGSVHWVDGVEEGP
                     KMADYTGAWYRIYDELDSNVPYYVPAIHMRVLPSSYFEPISPNVPYEEKLIEVNLSTQ
                     TLVAYEYGKSVFQTNISSGIPGGGLGGDKGISTTTPNGKFTVLDKVPAKHMGYSYFSI
                     GKGGDVTTDESGYVLPGIPWSTFFTPVGHAFHGTYWHENFGQEMSHGCINMRSNEANW
                     IFRWVQPNHSPEAISNHRGRGTMVEIHY"
     gene            22970..24202
                     /locus_tag="PL000_10550"
     CDS             22970..24202
                     /locus_tag="PL000_10550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC89385.1"
                     /translation="MPILNWKGKHPAAPEPASLIQDAILYPKGHGYPKTRPSGRVILG
                     ENLSVMAALLPEYEGRIDLIYADPPFFTNRKFSARIGRGEDSRKPHKWKLAEGYHDAW
                     PDLDSYLQFLYERLALMVRLLSPNGTLYLHLDWHADAYARLILDELFGSTGVFINEII
                     WAYHGPSPIKTAFNRKHDTILSYAKSGDYTFNVDAVREPYNPSTINTFKSSRKAGFGK
                     VPNLERGKVPEDWWYFPVVARLHNERTGYPTQKPEALLERIILASSNKGDLVADFFCG
                     SGTTALVAAKHGRKFITSDEAVRAVNTVRGRLVEAKSVFTVDRDESIKSVFLDGAKKV
                     KAHVLENSIRLETSLDVDFWEVDPDWDGKTFKSAAQAHRHARSGEIPLELEIKIGPKH
                     NACIRLVTVQGTQYQLYI"
     gene            complement(24191..24847)
                     /locus_tag="PL000_10555"
     CDS             complement(24191..24847)
                     /locus_tag="PL000_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89386.1"
                     /translation="MKTTTLLVIEVHHSEIPSFAPDLQKKGFDVQIAPNGSKAVARLK
                     DVSPSLVVINAASLRSTGLRICQAIREKDAKLPIILIVENDGDVSKDAADAVLGLPFT
                     VQKLVNRIKPLLPGDGKNVLHVGPIRLDLEKRRVRCLGKSTKLTPRLVTLLHLLMDKH
                     GEVVEREPLFKKAWETNYTGDTRTLDVHISWLRRAIELDPDNPKFLKTIRGVGYRLDI
                     "
     gene            25148..25597
                     /locus_tag="PL000_10560"
     CDS             25148..25597
                     /locus_tag="PL000_10560"
                     /inference="COORDINATES: protein motif:HMM:NF023075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HNC89387.1"
                     /translation="MNTKHKGDIAEQAAVLEALKRGWGVLHPVGDNLPYDLVFDVFGV
                     LIKVQVKCAWFDVPSQNYVVDNRRTKTNRRSMIRDEYTETDFDFALVYLPDCSLFYIF
                     PVNVFISYGSEIHMTETDKRQRRPRSFEYRDAWKLIQQWAANEETHS"
     gene            complement(25862..26644)
                     /locus_tag="PL000_10565"
     CDS             complement(25862..26644)
                     /locus_tag="PL000_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89388.1"
                     /translation="MPPSTIGGINLLPESEKRAIYSRYIPTALLEKYNLPPLTSAAGY
                     NLLQFRFATGSTDVEMRLFHQVDFPDPILYAHLTDTLNGQIHVLLYILNDPASPRFNV
                     DRMPDGSPTKFGTLQRNIPSETEALEAGLAPGQVRRGLRLLGQAIEAFEGFVAALGHD
                     MYFVEPLYYHNAVIFERYGFSYQVGKRLMDSIHAGFQNGGDLAAKLDGSTPFRHPEAA
                     NGIRKRSWAIHDGLLDEPFTNVTMYKRVGKSAGINTTPDCKW"
     gene            complement(26718..27179)
                     /gene="smpB"
                     /locus_tag="PL000_10570"
     CDS             complement(26718..27179)
                     /gene="smpB"
                     /locus_tag="PL000_10570"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNC89389.1"
                     /translation="MTEDIKVIATNRKASFEYFLMEKFEAGIVLQGSEIKSVRAGQVS
                     IQEAYVDISDGEQAWLVEAHIAPYEQAGKYFNHEPKRKRRLLLHKKQIRELWNNVRIK
                     GNTVVPTRVYLKDGRAKIEIALAKGKKAYDKRATIAKRDEARSNERATRVR"
     assembly_gap    27205..27304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27308..>28559)
                     /locus_tag="PL000_10575"
     CDS             complement(27308..>28559)
                     /locus_tag="PL000_10575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89390.1"
                     /translation="MRFNFFQQSDTPQKYRANFTNLYLDIAWFGVLSGTSVNFLNVYA
                     TRLGATSFQIGLLAAMAAVVNLFLAIPAGRWIEKRHTGRAVFWSSVLFRIGYLFWIPL
                     PWLFDEQGQIWALIILAFLMAIPLTPLGVGFNALFAESVPERFRAQVAGTRNVTFAIA
                     YMLTSLIAGAILKNLPFETGYQVIFAIGAFGAAMSSYHIYHVTPLEETAPLPAIPTPA
                     PTPQTDSPRSLHTTLRLDIWNTKFRNVLLVLFFFHLTHFLPTPIYPLYNVRILNLNDN
                     NIGIGTAIYYLTVLIGSTQFRRIAHRYGNKKVTAWGAAGMAIYPLLLAFSGNIWQFYG
                     LSFIGGFLFAMINGAYINYMLENIPPNDRPSHLAWYTIILNTAILAGSFLAPAIAETI
                     GLANALIFIGILRLLAGLSVHKWG"
BASE COUNT         6832 a         6639 c         7456 g         7532 t
ORIGIN      
        1 gttgatgatt tcaatctcga tattgaaaag ggtgaattcg tttcctttct tgggccatca
       61 ggctgcggca agaccacaac cctgcgcatg gtggctgggt ttgaaattcc gacctctggc
      121 aagatcatgc ttgacggagc agatatcacc gataaagccc cgaaccaacg taatgtcggt
      181 atgatcttcc aatcgtacgc actcttcccg aacatgaccg ttgcccaaaa catcggcttt
      241 ggccttcggg ttcgcaaaga gccggaagcg acgattaaac agcgcgtgga agaaatggtc
      301 agcctgatca atcttgagaa acatgcgaat aaatacccgt atcagctttc gggtgggcag
      361 cagcagcgtg tctctttggc acgtgcgctg gccatccatc cgcaggtgct tttgctggat
      421 gagccgcttt ctgcgttgga tgcgaagatc cgcatctctc ttcgttccga gatccgtgcc
      481 attcagaaaa aattgggtat cactgccatc ttcgttacgc acgatcagga agaggccttg
      541 tccatttccg accgtatcgt ggttatgaat acgggcgtga tggagcaggt aggtacaccc
      601 tttgaaatct acaacttccc caagactcgc ttcgttgcca atttcgtcgg ctctttgaac
      661 actgcggatg cggaagtggt tgaccctgcc aatgggctgg tcagcatgga tggcgtccag
      721 tttgagtctg ctgaaggcac agaaggtcgc aagaaaggcg ataaagtgaa gatcgccatc
      781 cgccctgagc gtttgagttt tgccgccgat caaaagaagg ccaatgtagt tgatgcgacc
      841 atcgaaaaca tcaccttttt gggttctgtc gtccgtattc aggtgaagat cgggaatacg
      901 aaattcaata tggacacctt caacaaccca ttcctcgaat tgcctgcact tggttccaaa
      961 gaacaggtga cctgttcaaa ggaagccgtg ttggtgctcg acgaatagaa gatcccatca
     1021 aagtcaaaac ggttcgttgc gcggcgcgct tcgaaccgtt tttgtttgag gaactaaaaa
     1081 tgaacaaagt cattcttgtg ctttcagatg ctttacgtta cgacgttgca aaaaacaaca
     1141 tgggcttttt ggggcacctt gttgaaacca aacaggccag cctccataag atcgtagggg
     1201 agttgccaag catgtcccgc ccgatgtatg aaaccattca tacaggcatg ccttccagcg
     1261 agcatggaat tgttgccaat tcgatagtca gactctctgc caagccgaat gtatttcagg
     1321 cgttgaagaa tgctggaaag accacagctg ctgcggcata ttactggtat tcagagcttt
     1381 acaatcgcgc tccctatcat ccagttgatg acaaggagac cgatgatgaa cggctcaata
     1441 ttcaacatgg ccgtttctac acggaggatg aatatccaga tgttgagctc tttgccagtg
     1501 ctgcacacct gatccgtaaa ttttcccccg attatttgct gcttcatcca atgggcatgg
     1561 actatcatgg cgagaccttt ggcgctgatt caaaggaata tcgcaaccat gccatttatc
     1621 aagattcaaa acttgcgcca ctcatcatgg aatggaagga acgagggtat attgtctttg
     1681 ttacgggaga tcatggcatc aatgccgatg gcgggcatgg cggtaccacg cccgagcagc
     1741 gcaccgttcc attttttgtg attcaaccgt cgggtagggg aagaggcgag acgggtgagg
     1801 tcatttctca tttgcagatc gcccctacga ttctcaagtt actgaacgtc ccaatccctg
     1861 agtcgatgaa atatccgccg ctatccctcg actagcgcct ttacaagttc gcggttgaaa
     1921 tcgggaatgt ccgcaaccac ccgaccccat acctgattgc cctcacgaaa ggcagcttca
     1981 tcagcccaaa ccgcacccgc gttgacaagg tcatccttga tgcccgttga ccccgtggcg
     2041 cgctgacctt ttttgatgat gccagcagaa atggcgatcg accctccatg acagatgatg
     2101 ccgatgatct tttcggcatc atttattttt ctaaccagat ttttcaccac gtcgtacctg
     2161 cggattttat cgggcgccca gcctccgggg agaatgattg caaacagttc gtcagcattg
     2221 agcgattcga ttgtcgcatc gggggtaatg accagaccgt tttttccgcg cacggatttc
     2281 atatccagac cagcggaaat gacctttgca ccttcctcct gcagacgcat gagcggaacg
     2341 taatattcca gatcttccac gccttcagcg atcaatgtgg cgatcagttt gttttgtaaa
     2401 cgcatgttga aatctcctcg gttgttaagt attgataaat atttgaatag acgttgaggc
     2461 tttattcgcc gatgattttt accagcacgc gtttgcgacg gcggccatca aattcaccgt
     2521 aaaagatctg ttcccacggg ccaaaatcca atttaccctc tgttacagcc accacaactt
     2581 cacgtcccat gacctgacgt ttcatatgcg cgtcggcatt gtcttcacct gtgtcgttgt
     2641 gacgatatcc gctgacaggt tcatgcggag cgagtttttc cagccacttg tcataatctt
     2701 gatgcagccc tgattcgtca tcgttgatga aaaccgaggc agttatgtgc atggcgttga
     2761 ccaaaataag accctcctga atgccgcttt cttcaaggca ggcttccact tgtgctgtga
     2821 tattaacaaa gccgcggcgc gcagggatgt tgaaccatag ttctttgcgg taggatttca
     2881 taagattctc gtgtttcgtg tgatgtgatt tcttttgtgg taattataca gtgcttatgt
     2941 agccgtgttt tgatcacctg caacgacatt cctgctgaaa gtccctttga gttcaagagc
     3001 gaggttgtga taggggattc aatttccagc gcgcactgat aagaggggat gttttttgtc
     3061 agacatggtt aagtcatttt ttggggggat ccccgctttt cgttaaatta tacgacctct
     3121 tttgatatat agtctctgct ttttgattta ttgaagattt ttacatagtt atacttaatt
     3181 ccaaaagcag actactggtc aacctccttt tggaaccata aatcagcggg aagaaggtga
     3241 aaaattaagt aataatacct atggtaatca ttggattgtt tagataatat atctgcagtt
     3301 ccagtatgta acggtagatg ggctatcgaa aaacttgcct ttcccgaaac gttctggcga
     3361 aaaaaaacac tatttcttgg gagcggttat gcatccagtt acgatccttc tagtggatga
     3421 tcataaattg atacggcagg gaatgcgttc tttgttggag actcggaaag gttattctgt
     3481 ggttggtgag gcgggcgatg gacagggtgg gcttgaaatg atcgaacggt tttcccccga
     3541 tgtagccatt ttggatgtaa tgatgcccgg tctgaatggc attgaagtta cacgactcgc
     3601 ccgtcaacgt gggtttaaga caaaaattct ggttctgagc atgtttgcca atgccgctta
     3661 tgccgtccgt gctttgcagg gcggcgcctt ggggtatgtg ttgaaggagg cggatttttc
     3721 tgagattctt tgtgccattg atactgtcat ggattcgaga aggtatctca gcccggagat
     3781 cgccgaagag gtgctcgaaa tgctcctgca gacggggaat gaaaaagaaa gcgatgtact
     3841 gaacctgctg tcaccccgtg aaagggaaat attacaactt gttggcgaag gaaagaccaa
     3901 tgctgtgatt gctgataaac taatcttaag tgtacggacg gtggagtcgc accgttttaa
     3961 tgcaatgaaa aagttgcgca tcaattcaca tgcagacctg gtaaagttcg ccatcaatgc
     4021 cggtcttgtt acatctgagc cttacatttg aaaataattg aacaaaggga aaaatgacca
     4081 atcaactacc aaccatccta gtcattgatg atgatccgat tgtcaggcag accataaaga
     4141 cccttttggc aggggatgac gttcgcctgg cgttcgcaga agatggacca agcggaattg
     4201 aaacagcgca aaaactcctg ccggatgcca tccttttgga tgtgatgatg ccccgcatga
     4261 atggctatga tgtctgtcag gcgcttcgcg cagaccccag cctgagtgag gtgcatatcg
     4321 tcatgctgac ctcgctggat gatcgcgatg cccgcttgac cggattcatg gctggcgtgg
     4381 atgatttcat caccaagccg ttcgacaggc ttgaactgca tttgcgcttg aagaccatca
     4441 cccgcattaa tcgatatcgg cgcttgatgg ctgagcgggc ccgtttttca tggatcgttg
     4501 aaaacagcta cgaaggttat ctgctgctta gttcggatgg aaccattcaa tatgccaact
     4561 caagcgcaca gactttttta caccttcccc gtgagtgcgt tgggatcaat atcaccagcc
     4621 atgttgtaaa acattattca ccctacccaa cggaaacctg gactgcctgg ctcgataccc
     4681 cggcgccgtg ttatcttgtt cagcctgaaa ccgccaatgc gcgggccttt tggctgctgc
     4741 tggaagcgat cgacatgccg gtgaagagtg acaacagccg tgttgtacgt gtgcgtgatg
     4801 tgaccgaatt gctttccaca catcacgaca tgcggaaatt ccataaagcg gtgacgcata
     4861 aactccgctc gccattctcc acgcttgtga tgagcatgag catgctcgag aacaggcttc
     4921 aaaaactttc tgaatccgag atcaaaggat ttgtatccct gtctctggag gctattaatt
     4981 tcctggcggg tgatgtgaac aaggttttgg attacatcga tacaccgctg gcgatggaga
     5041 gtaatattcc gcttgaaatt cgattcctgc ccgaaatgtt gaaatcgatc caaaacgaac
     5101 agggacttgg caacatcaac ttgacgattc atcctgacct gatgactgcc tgcctcggaa
     5161 ttacagatta tgccttcgag ttggttctgg gagaattgat cgaaaatagc aagaagttcc
     5221 acccagatca cgctccgcag gtggatgttc tcgttgagcc tgtggaacat gagaacatgg
     5281 tcagggtgac cgtgatggat gatggaattc gcctgacggc agaacaattg aaatgggcac
     5341 gtatgcctta ttttcaaggc gaaaaattct acaccggcga aaccccggga atgggtctgg
     5401 gcatcccgac aattacctcg atcatttggc aggtgggtgg agaagtggtg atcaccaacc
     5461 gcgaagaccg cgatggcttg aaggtttcac ttcgcattcc gttggcgaat gaataaagat
     5521 ttatattcga agcgatcata attttgtagc agataacagc agggttgaga tatcatctca
     5581 accctgctgt tattgtttgt aaatggttgt tatcttctgc tactcaatag gtgccacgca
     5641 accggggatg ctttttccat ccctcaattc agcgcggagg tatcgttttc cacgcgcagc
     5701 aagcaggttg caatcagaga ccaacccaca actggcacaa tgacctccgt ccgggccgat
     5761 ttccagcagt tttcccttct gcacaagata tgcgatcatg gctaacacgg cagtgggttg
     5821 ggcgttcaag gcgcggctga tttctgaaag gcttagaccg ttttctttat cttccagaag
     5881 ctgtaataat tggcctaaca ttgagttatc caaagccaag tattttcccg ccttgataaa
     5941 caaggaaggc cgtgcaccag gcaattgctg tttgatacag ggcagaggtt accgcccatg
     6001 aattaccaaa ttcatgtttg atcgccccta acgttgccac acacggtaca tataacaaga
     6061 caaagaccag caaggcagcc gcgctcagcg gcgtaaattc gttgcgcagg gcaatgctga
     6121 gaacagtatc ttcggtttgc tcatcttcag gaatcaggtc tatgcctggc agaatgctga
     6181 ctagaatttt gcctgaattg atggtggctt ccgcaaaacc cttgccggtg ctgatgatct
     6241 cctcgctgaa cgtggcaggt tcagcgttgg ccgtggtttc gctgcctgtg taaaccaaac
     6301 tcatggtgct gaccacgatt tccttcgcgg caaaccctgt caccaaggcg ccgctggttt
     6361 gccagttgcc aaaaccgagc ggtgaaaaga tcggggcaat aaatccgctg acctttccga
     6421 aataggaatc tttttgattg gtcactccca gcggaaggtt caataacaac cacatgatga
     6481 ccgatgcccc aagaatgacc gtgcccgcct tgcggacaaa ttcacgcgtg ttctcccaca
     6541 tgtgaatgag aatgcttttc attgcaggct ggcgataggg tggcagttcg agcacaaagg
     6601 cggaagtgga atcaggcttt agcagggtgc gggtgaaaac catgccagcc agcatggcca
     6661 caaggattcc aagcgcatac attgcccaga tcactgtccc tgctttactg ccaaagaatg
     6721 ccagaccaaa tacgacgtag acaggaaggc gtgctgaaca agacatcagc gggacgagga
     6781 gtgcggtcaa gatcctatcc cgacggctgg caattgtccg agtggcatac accgcaggga
     6841 cggcacaccc aaacccaagg atcatcggga taaaagattt gccgtgcagg cccaccacac
     6901 gcataaatcg gtccatcaca aaagcggccc gcgacatgta ccccgaatct tccagcaggg
     6961 ccaggaagaa gtacagaatc aacagcccgg ggacaaagac cagcacaccg cccacgcctg
     7021 ccacgattcc gtcaatgagg agagaatgca tccacgcggg agcctgtacg aggtccagca
     7081 ggaacgagaa aagatgggcg atcggaccgt tgatgagggt gtccgtccaa tccaaaaacg
     7141 gagaggatac gtcgatcacc aatcgaaaca tcacatacat gatggcgaag aaaaatggca
     7201 gccccagcca tttgtgtgtc acgatgtcgt cgatacggtc ggtgagggtg atgcggtcaa
     7261 tgagcggacg ttgtagcgat ttgcggacga tgccattgat gaagccatag cggcggtcgg
     7321 cggtgagcag gtcaatttct tcgcccaatg tggattcaac ctgttttgct ctttcaacgg
     7381 caaattgaat gacttcacga ccgccttgca tggattggat atgttcgagg atatctgaat
     7441 ctttttcaag aagtttgatg gcaagccagc gtttggaata tttccccgta tcgaatttca
     7501 tgctttcaaa gatcgccagg atgctcgcga tctcggtttc gagtgtttcg ccgtaatcaa
     7561 ggcgaaaaat ggtttgcggt gtagggtgca ttatgcggtc tcttttattt tgcgtgcaga
     7621 ttcaattgct ttggtcatca agtctgaaat gcccctgcct ttgtttgccg ctgtgaacag
     7681 aatgggaatg ccgcccagca tttgcgagag gtgctctgtg ttgatttttg tgccgttctt
     7741 ttgcagggca tcggtcatgt tcaacgccag caccacgggc acgtcgagct cgagcagctg
     7801 cacggtgagg tagaggttgc gttcaaggtt ggtggcgtcg atcacattga tgaccacatc
     7861 gggcttttct tgaataataa aatcacgggc aatgatctct tctggcgaat aagctgtgag
     7921 gctgtacgtt cctggcaggt caacaatgtt gatgctcata ccgttgtgct cgatctcgcc
     7981 ttcttttttt tcaacggtct tgcctggcca gttacctgtg tgctggtgca gacctgtcag
     8041 caggttgaag atggtggtct ttccagcatt tggattcccc gccagggcga ttgtcaggtt
     8101 tgccatcaat tgcccccgtc gagattgact tcgatctgcg cggcttcctc tctgcgcaat
     8161 gccagatgat agcccttgat gaagtattca acagggtcgc ccagcggcgc attgcgttcg
     8221 atcaggatcg tttccccccg cacaataccc atttcaacaa agcgacgccg caaagcgccc
     8281 gctccgttga tttgcacaat ggtggctcgc tgcccaggct gtaataaatg caattgagtt
     8341 gagcttggca tgattgtttt ccttttgtta atttgttttc acgatgacga actccgccat
     8401 tgaaaggccc aacgcggttt cttttccgcc agccttcaat gtcagcggac cctgcaacgg
     8461 tgccgcttct gtgatcgtta tttttttacc tggcagaatg ccgcgttcaa ataaataagt
     8521 cagcacatcg gtggcggtgg catttacagc caatacctca accgttgcgc cgatgggtgc
     8581 catgctgagc ggtgtacctt ccagcggtgc aaacgtccca tctgctgcag ggatcgggtt
     8641 gcctcgcggg caagttttcg ggtcacccaa aaaaactgac agcgcttctg tcacttcggg
     8701 tgctgtggca tgttcaagcg aacaagccag ttcatagatt tttgcccatt cgatcttcaa
     8761 atgttcgtac aaaaaacatt cccacaaacg ttgtcggcgc agcacattgc aagccgcctt
     8821 gcgtcccttt gccgtcaatg acacgccctt gtaaggtgta tgtgccatca aaccctgctc
     8881 gcttaacctt cgcaccattt cattcgctga aacaggcgtc acatttaaac gttctgccaa
     8941 acgccccacg gccacttttt cgcgatcgct taattcaagc atcgctttca agtacatttc
     9001 cgtgctttcg cttagatact gaatggtcat aaggttggcc tctcaaaaat gttatggctg
     9061 accttattat ttcaggaatc gctgcatttg ggcatggaag gatgtcacat tcttttggga
     9121 aggaaatccg cggttcccac ccgacctgaa acctgattct gttaaataaa aagattctcc
     9181 ccgtagggag aatctacaaa aacctcaagc caatcgtcgg gttattttgc ccgatgaacc
     9241 gccaatacct gctcaatttc tttgatgtga tactcacgat gtccattgcg ctcaaacttg
     9301 aactgaacat tggcatcctg tatctttttt accagatcat ccgaggcggc ttcgatttcg
     9361 ctgtcaattt cctgggcggc tttcagtgcc aactctgcag cagttcgggg tggaagtgcc
     9421 atgcaaagcg gcttttgggc ttcattgatc gcatccacat cgtagggaga ggggacgatc
     9481 tcattttcac gccacagcct caacaggtgc aggattcgaa aatcccaaaa ggccacatgc
     9541 gcaaaggttg cggccacggt ccagcctgcc acagcaggca gagccagttc tgcgtcggtc
     9601 atcccttcga tcagttcacg cattttttcg cgtgaggttt tgttcttaat ttgaaaatct
     9661 ctgttcatgc ttatttgcct ttcggaaaaa tcgcgcccgt tttttgcata taggttcgat
     9721 attcatcgcc aaatctatcc agcatcatct gctcttcagc acggacccgc aaaatataga
     9781 acggaataaa aatcaggaaa ttgagaaacc ctgccagcca gttttgcaac aacagcggct
     9841 gggcaatgac ccaaacccat tgtgaagcat acattgggtg gcggatatac ccaaagatac
     9901 cgtttgtgat gagagtgtgt tccttgcgga tttccagcga aggcgaccag ttcaatccca
     9961 ggtctttgtg tgagcgccag aaaataagca gggcaaatcc catcagaacc acgccaagcc
    10021 agccagccca attcggcagg gagtaattgg caaaatccag ccagttcgtg gtcgaataga
    10081 tcagcgggag caggaacatt ccgacgaaca aaaacccaag gatggtcttt tcctgtgcag
    10141 acactctttg ctcagatttc gcttctttcc gttgttgcct gctgatcggt gctcgaatga
    10201 ccatttcggc aatgatggct gcccaaaaga cgagtttgaa aattgccatt gtttatctct
    10261 ctacgcgctt ttttccacta cgatcgtggc ctcggtcagc aaggccgcaa ctgcaccgat
    10321 ggcggccagt gtgggtgcaa gcaatactcc caccacacca aaggtcagcg gaatctccat
    10381 gatggtcttg ccttccttgt ctttgatgat gatacggcgg atgttcccct catggatcaa
    10441 ttctttgatc ttggcgatca atttttcgcc gtctaccttg aattcctctg tgcgaaaatc
    10501 atcgggcatg ttgggactcc tttactcgaa ccatttctcg tccaacgcag gccattcgtc
    10561 tgattggtcg gcgcgggctt cccagttttc ttttccgctc cacgattcga ttttgatcgc
    10621 atagaccgaa gtggcgcgca attccttgtc ggtgatctcg cggtattctt tgcctgcggt
    10681 cattttaggg aagtatttac tgatcaagcc atagagcagt tgtttggcct cgtttggttc
    10741 gctgacaaga cgtgcctcac caaaaacgac cgcactgcgg tattgaagcg agaattcaag
    10801 cgccacattg gagggtagta actttcctag ctcactgact tcgaggcata cttttgggtt
    10861 tgcttcgatg tttgaacgca cccgccccgc aatattcgag tggaacacga tctggtgatt
    10921 ttcttcatcg aaccagaatg tggttgggtt gataaatggc tggttctcat aggctgaggc
    10981 gatatgcccc acttgtgcaa ctttaagaag ggccgcgatc cattcatcgt cacgggttag
    11041 atgcgggcgg cgttgaaatg cggtgggcgg ttggccaagg tagttacgag tcatggttgt
    11101 ttgaaaattc cttttgcaag cgtttttttt ccagcactaa ttgctttttg taaacccata
    11161 tctgaaggaa gaatgccagc aatcccaatg cgatggagta tgcccaattg catggcgcgc
    11221 aattgtattg aatccctttt tggaagaaaa agaccccggt gccaaaggtg agtgcgctga
    11281 gaatggcaac ttccactgtt tgcaggaagg agacgctgtt gggtttgaag aattcagggg
    11341 cgttttgaat ccgccacctg aatgcccctg caagtgattt atctctggcg atgatgaatt
    11401 ctttgatcct gtccatggcg atcagtgatt cgaaccaagc cacacgcaga cgggtcaact
    11461 gcagaatggt ggttgttccc aacagggtga gcagcagaaa aaggatgctg aacaggatat
    11521 tgatcaggtc agggttgaac acgttgttta ggaattgtgt gctgaataat gcagccacca
    11581 acgaaccaac cgcgaccaga taaaaggatg acacacgggc acggtcttca ttcgactgtg
    11641 cagctgttgc agcaatatag ttatattctg cgatcagaag atccatcgag tcttttgaaa
    11701 ttggggtttc ttttttgggg gattttgcag gcatgctttt attataacgc cgataaaaaa
    11761 agcccgatgg cggtgaccat cgggcgtgga gtgcggtgtc agacggttag gctttgaagg
    11821 cccattcacc ttttcgcatg aggggttcgc gtttcccgtt tgcgaggatg ccgtcaatat
    11881 ccatatcctt tgatccgatc ataaaatcgg tgtggatgat gctgtgattt ccaccgcgtt
    11941 tttggaaatc gccttgcgat aatttgttcc catctttcat tgtgaagggg taggcggctc
    12001 ccagcgcaat atggctggag gcattttcgt caaagagggt gttgtagaag aggattccgc
    12061 tctggctgat gggggagcta tttggaacca gagccacttc cccgaggcgt gcggcgcctt
    12121 catcggtttc gatcaatttg cgcagggttt cctcatgttt ttttgcagtg acttttacaa
    12181 ccctgccttt ttcaaaggta agggtgaaat cttccacagt tgtgcctgca atgaccaggg
    12241 gacgtgatgc agccacaaaa ccatccacct tatccttgtg aggcatggtg aatacttctt
    12301 cggtgggaag gttggcggtg aagtcaatac cggcctggct tttacttcgg gctgcgaccc
    12361 atttatgttg gctgggcagg ccaacggtca ggtcggtgcc gggggctttg tagtgcaggg
    12421 ctgcatatcg ttttttgttc aaataggcac ttcgaaggct taagtcttta atatgttttt
    12481 cccaggcggc aaccgggtcg cgctggtcta cacggcaggt tttgaaaatg gcttcccata
    12541 atttttcctg agcttgtttt gtggaggttt tggggaagac cttttttgcc caatcttcag
    12601 cggggtagga aatcacacac cagttgatgg cgtcgcgcat cacagcttgg gtgagtttgc
    12661 gcatttttgt acccatggtt ttgcggtaaa tggcaatggc ttccgggtct acatctgcaa
    12721 gcaggtctgg gttatcggag gtgatgccga tgtaggcatc tccgttcttg acaatgcttg
    12781 tgtaggcggg aacgatccat gaagggactt ctgtgagatt tttgtgcgag ctcattttca
    12841 tgcgggtgtg aaaaacctcg tcgtctccat aaaaaacggt gacgagtttt gcgccactgg
    12901 cataggcgct ttttgtaatg gctcgtacca gcggataatc ggtgatgctt gcagagatgg
    12961 tcaggttctg gcccttgcgc aggttgatac cgactttgac gatcacatcg gcatattttt
    13021 ctaacgattt ttgaaattca gcagtattca tgtagcctcc attgtatggg caagtttgtt
    13081 tgttgtcatg ttcttatacg ctaaaggccc attcaccgtt gcgcatcagg ggttcgcgtg
    13141 ttccgtcagc ttgaattccg tcaatatcca ttttggcaga gccgatcatg aaatccacat
    13201 ggatcaggct tttgtttccg ccgcgtgctg caaattcttc atctgtcatg tccacgccgc
    13261 cctgcatgga tgatttgtag gcgctgccaa gggcaagatg gcaggatgcg ttttcatcaa
    13321 aaagggtgtt gtaaaacagg attccgcttt ggctgatggg ggagctgttg ggtaccagtg
    13381 ccacctcgcc aagacgaccc gcgttttcgt cggtttcgat gagtttgcga aggtcggcat
    13441 ctcctttttt tgcagaaaaa ttgaccgcac gtccgttttc aaagcggatg ctgaattctt
    13501 ccatcaaaac accctgattg ttcagtggga gtgtggctgt taccagaccg tccactttat
    13561 ttttgtgagg catggtgaat acttcctctg tgggaatgtt taccgtgccg gtaatgccat
    13621 tcttgaaggt ttcctgtgcg ccgccccagt gatggtcttc aggcaggccg atcgttaggt
    13681 ctgtgccggg agctttgtaa cgcaggctgg caaatcgttt tgcgtttaga tagtttttgt
    13741 atttatccag tttttgaatg tgctcttccc aggcttttac cgggtcgggg gtgtggatgc
    13801 ggcaggcgcg aaagattgca tcccataatt ttgtttgggc ttctttttca ggcaggttgg
    13861 gaaagacctt ttgtgcccag gcgggtgcag ccgtggaaac tacacaccag ttggcgtagt
    13921 tgttgtattt gcgtagcggc tcgacgattt tttgataaag ggtttttctt gtaattgcaa
    13981 ttcggtcgga gtcaattcca ttgtataggt ctgggttgct ggagagaatg gcgagttcgg
    14041 catctccgcg tttgctgtat tcttcgtaac ggttatagag ccaatcgggc agggtggtga
    14101 gggtttcagg gtcggcgtgc tgcaagcggg tgcgtttgat attttcatca tcccataaaa
    14161 cgtccacata tcttgccccg gccttgtatg cggtttcggc gaccttcctg ataaatgggg
    14221 cgtcatcgac atttccgcga atggataagt gctggtcgtt cctcaaattc aagccaactt
    14281 ttacgacaac ttcggcgtat ttttcgagca gttgattaaa ttcctgatcg tccatgattc
    14341 accttttgtt tttaaagata aaacctcctg ccgatcttga tttcggcggg aggtccgttt
    14401 tacgcttcaa ttgcccattc accctgacgc atgactggtt cgcgagaacc gtcctttttg
    14461 ataccgtcaa tgtccatctt tggagaaccg atcataaagt caacatggtt gaggctgaca
    14521 ttgccaccgg cgctgacaaa ttcctcgtcg gtcagttcct ctccacccgt aagagtgaag
    14581 cggtatgctc gtccgattgc gatatgacag gaggcattct cgtcgtacaa ggtgttgtag
    14641 aagagatgtc cgcgtttggc gatgggggag ctggcaggga caagggcgac ttcgccgagg
    14701 cggtgcgagc cttcatcggt atccaccagt ctttgcaggg ctgcttcgcc ttttcttgca
    14761 ctgactttga cgatacggcc tttttcgaaa gttacctgaa aatcttcgat caaggtgccg
    14821 ccgtaactta gcgggaaggt ggatgtgaca ataccatctg cgcgatttct atcgggcagg
    14881 gtaaatacct cttcggtcgg catgttggcg gtgaacatga cgccgttttg cgccatggat
    14941 tgtgcgctga tccattggtg accactggga aggccaagtg tgaaatcggt gccaggggcg
    15001 gaatagtgca ggcctgtgta ttgtttggct tgcagatatt tggcgcgttt gcgcagattg
    15061 acgatatggg tttcccaggc ggcaatgggg tcaggctggt caatgcgggt ggtttcgaag
    15121 attgcatccc ataatttttg ctgtgctgtt tgtgcatcca gatctggaaa gatcttcttt
    15181 gcccaggttt cgccggcagc agccacaaca caccagttga ttgcattggt ggtgaccttt
    15241 tcgctgactt tgctgtaata ctgcaaatgg gttctttgca tggccccgac cttttcaggg
    15301 tcaaggctgc caagcaaatc tggatttgag ccgccgatgg agaggagtgc atctccattt
    15361 tcgatcattt tcaagatcgc atccacttgc caggttgggt attcgctaaa ggagtctttg
    15421 ggggcgtgct ctgcccgcag acgcatcatt tcttcatcac tccatattac ttccacatat
    15481 tttgcacctg cggcgtatgc ggccttggta acttcgtgaa ccaggggtgc aaattgatag
    15541 ggaacaccac gtgtagcggc gagggtgatg atcaaacgtt ggccagcgcg aatatttaaa
    15601 cctactttta cgatcgcttc agcgtattgt tttaagagct cttggtggga ttttccaggc
    15661 atggggtctc ggtagaagga ttttttgcaa gtatagcatg gacgtgctta ttttgcccct
    15721 aaaaatactt tctatttcat atttaaccaa tccgatttat tgcctgtctg tcaggaggct
    15781 tggaacaaaa atggcctgga tatgtttgcc aagccatttt tatttacact tgaaattgaa
    15841 caggggtgct tgttttttgt tcttcgcctt cttcgggttt ggtcagtgga aggattacat
    15901 gaaattgact gccggggaag ttgatttcat cgtaaccggg gctttccaca taaatacgtc
    15961 cgccgtgcgc ttcgacaatg ccttttgcaa gggcaaggcc tagtccggcg ccgccgccct
    16021 taaaacgtgt tttgctggtg gagtgtttgc ccagttcgcc cggttggtag aatttcgtga
    16081 agatgatttc acggaaattt ggatctacac cgacacccgt atcagcgatg acgatttcaa
    16141 gaccgccatt gggcaggtca atgattgcgg ggatgctgtg gccggtgacg gtgatcttgc
    16201 cattgttggg tgtgaatttg accgcattac gaatcaagtg atggaacaat tttcgcatca
    16261 ggttggggtc gcatttcagg tggggcagac tgggcaggtc aatgctgagg gagtgcttcc
    16321 gctctttgat ggaggaagcc tgctcgatac atacttcctg gagcaaatgc cctatttcaa
    16381 cgggctggat gtgtggtttt agggaacgcg catcgatctg tgcgatgtcg aacatcgagt
    16441 ccatgacttc gtgcaggcgg atggtgccgt cgtggatgct gttcatgatc tgcttgtaat
    16501 tggggtcgag gctggtatct tcgagcagca tttcagtata gcctttgatg acggtcaaag
    16561 gggtgcgcag ttcgtgcgag gcgatactga tgaaatcgga tttagcctgg tcgatgcgtt
    16621 ccagatcgcg attatttttt tcgagagcgc ggcgtgcctg acgcagttcg ttattcagcc
    16681 gcatgaggtt atcaaccagt ctggcatttt caagtgcgat ggcggtctgg ttggcgtgtg
    16741 aactgagggt gacgaggtct tcactggtgt agcggtttcg gctgagtttg gggccaaagg
    16801 ccagcaggcc gatccattgc ttcttggcaa agatcggaat atacacttca gtgtggagct
    16861 ggttgaacca gtctctttca aagggtgaag cagcccgaaa cgcgggaagc aggtccaggt
    16921 catattgaag aagcggtttt tgttcgcgga tgaagtacga ggcgatgatt ccgttttcat
    16981 ccaattccac tgcgatcatt tgacgttctt cagggctgcg ggctgagcga agtctgtagg
    17041 ttctatgccc atctgcttcg cggtctgagt ccaccaaaaa taaaaatccg cgctcgattt
    17101 gcatggcttc cagaatgatg cccacggcaa tgctggcaag gcggtccata tccaaaatat
    17161 tgctgatgct ttcgctgtat ttgtgaatgg tgtggctggg gttgtattga tcgcccttga
    17221 cccaattgtt taccgcgcgt gaaaccaggt tcaacattgg tgcaaagatc aaggtcaaca
    17281 acagagcaat gaaagcgcct gcaaaaagcg gattgaagtt tttcgaattt ccaaaaacag
    17341 cttcaatcaa gaggtagcct gccgcatacg cgccgacaat gaccacggca atggccacat
    17401 aagccagaat gcgacggaag atctggcgca ggtcaggtac gttatgagtg ccgatgacgt
    17461 acaccatcaa caccgctgcc atcaggcgca ggggctggcc tgcaatcatg atattggaaa
    17521 agaggagaac atcattgacg aacaacagga aaacagtagg ccaccaatag ttcagacggt
    17581 tgcggaacag ttgctggcgc gcctgcttgc ctgcgtttga gatcgagatt accagcgcga
    17641 tcgaaatgat caaccaccct aaaattgccc aggagggtcc aagccgttca cggggcaggg
    17701 tgagtgtgcc attgctccac aacacatcgg gcagcttgag tgcattggtc aaaatcagcg
    17761 caaggccaag catccataac gtccacacac caacccaaac ccaccaggtc tcataccgca
    17821 tgaaattttg aaatgccagc atgaggattg cgatcaatgt gaacgcaata tagatctgca
    17881 cttcctgaaa aagaagtgta ccgccttgtg aggcttcaat ggcttcagca acattcaaca
    17941 ccatggcaag aagcgcaaaa aaagccgcca gccaaatgga gatgccgtgg ccgtcatcac
    18001 gcctttggat ggcgaagaaa atgatgggca ggtataaggc ccccagcagg agcaataaga
    18061 caaaaagtgt gggtgatgtg ttcatgcttg cagaaaggat tttacccgaa aaaattcgat
    18121 aatcggttat cattgttgtt atgctaattt cccgcgcatc ccgtctcaaa ttcaattttt
    18181 ctgtggattt ctttcgcccg gacggacacg taaccttcgc ggatttgacc gccgcccgag
    18241 cctttgccgc ccaaatgtca acgcctcgaa ccgacccggt acctggttct gacctatatg
    18301 cattctacct gctggatgag gcctaccaca tcatcctcaa acattatttc agcagataca
    18361 ccggcgtgat gggccgtgcc atgggggttt tgcaatcgaa ccttggctca aaatacgact
    18421 tgaccctcag taaatttatc gaagaatttc cacccctgtc tgtcttttta gggcagacaa
    18481 cggcgggtgt gtatcttgcc accaagcttc cgaattttgg cgattttggt catggcgtgc
    18541 gcgctgccgc cgtggaagaa atgctgctga tcaataactc caataacaac cctgcactat
    18601 cccgttataa agtgttgatt gacgaatccg tgatgaacgg ttcggcgtac aaggaactgg
    18661 tgcaactctt gcttgacttt tttgcccgcc agcctggttt tggcaatggt gaatttgcct
    18721 tgggagaaac cctcaacgaa ttactgcttg cgcccatcaa agcgtcacct gattcgcttg
    18781 aaggccaatt acaatttatt gttcaaaagt ggggacattt gcttggcgag gcgttttctg
    18841 caaaattact gcgcggcatg gatttcctgc gtgaagaaac catccgtctg caaaccagac
    18901 agatcgatcc ctcgcaaaag gtggagacct acgttcccag ttttgtcggc agtgaatatg
    18961 ctgaatatga acgctacagc cccgacaaag attggatgcc ccgccttgtt ttgctcgcaa
    19021 aaaattcgta tgtctggctt ggccagcttt cgaaaaaata tcaacgcgaa atttccgctc
    19081 tcgatcaaat tcctgatgaa gaactggatt tgctcgcctc gcgtggattc acaggccttt
    19141 ggttgatcgg cctttgggaa cgcagccgcg cttcccagat catcaaacaa cgtatggggc
    19201 aggccgatgc agttgcctcc gcatactccc ttcactccta cgatattgca agtgacctcg
    19261 gcggctggaa tgccttgaac aaccttcgct ctcgtgcatg ggcacgcggc atccgtcttt
    19321 ccgccgacat ggttcccaat cacatgggga tcgactcaac ctgggtggtg gaacaccccg
    19381 agtggttcct ctccacatcg aacccgccgt atccatctta ttctttccgc tctgaaaacc
    19441 tctccaacga cctgcgagtt ggcatttacc ttgaagacca ttattacgac aagaccgatg
    19501 ccgcagtggt atttcagcgc cgcgatctgc aaacaggtga tttacgctac atctatcatg
    19561 gcaacgatgg cacgtccttc ccgtggaacg atacagcgca attggactac accaaccccg
    19621 tcgtccgtga agcggtgatt caagtcattt tgcatgtggc tcgcaatttc cccgtcattc
    19681 gtttcgatgc ggccatgacc cttgccaaga aacatgttca gcgcctgtgg tttcccgagc
    19741 ctggggcggg tggggcgatt ccatcacgct cacaatacgg catgaccaaa tctgaatttg
    19801 acgaaaaagt tccagaggaa ttctggcgtg aagtggtgga ccgtgtcgct gctgaagttc
    19861 cagatacgct tttgcttgca gaggctttct ggttgatgga ggggtatttt gtgcgcacgc
    19921 ttggcatgca ccgtgtttac aactctgctt ttatgcacat gctgcgcgac gaagacaatg
    19981 ccaaatatcg ccttgccatc aaaaacaccc ttgagttcga tccgcagatc ctcaagcgtt
    20041 atgtcaattt tatgaacaac cccgatgaga agacggctgt tgagcaattc ggcagcggtg
    20101 acaaatactt tggcatttgc acggttcttt ccacgttgcc tgggttgccc atgtttgggc
    20161 atgggcaggt ggaaggcttt gctgaaaaat acgggatgga atatcgccgc gccaagctgg
    20221 atgaaacccc cgacgaaggc cttctccgtg gtcacgaatg gcggatcttc ccgcttcttc
    20281 accgccgaca cctgttcgct gatgtggaaa agtttctgct ctttgacttt tacacatcaa
    20341 gcggcaacgt aaatgaagat gtgtttgcat attccaaccg tcacaatgac gaacgcggac
    20401 tcatcgtcta ccacaatcgc tttgccaaca ccagcggctg gatcaaaacc tccgctgcgt
    20461 acattgataa atcttctggc agaaccttgc gcaagaatct tgccgaaggc ctcagcctgc
    20521 cgcgtgcagg gtacgtcatt tttaaagatt gttcctccca gcttgaatac attcgctcct
    20581 gcaaagatat ctgggaaaaa ggcatgtaca tcgaacttgg tgcctatcaa tgccacgcct
    20641 ttgttgattt tcgcttcgta ggcgatgttg aatggaagac catttgcgac cagttgaacg
    20701 gcgcaggcgt cccctccatg caggataagt ggaaggaaat gtttgccgcc aaagaagaga
    20761 cgtcagttgt ggaaaaaacc gcgaaaaaga aacgtgtggt caagaaggca gtaacgaaga
    20821 aaaaagaaac ggctctggcg aaggcgaaga aatccccagt taaaaagaag gcgacagtaa
    20881 aaaagcccgc caaaaagaaa gttgcaccga aagctaagaa ggctgttgaa acaaaacccg
    20941 cggcaaaagc aaagaagact gtttcgacca gtaaaacgac tgtgaaaaag acaaccaaac
    21001 ccaaaaaggg agttgaatct aaaaagccag tggcttctgt caaaaccagc aagcctgtca
    21061 gaaaaaagag ttctacaaaa gcgaagtaac tgattaagga agcctcataa aattattgtg
    21121 aggcttcctt ttttgtgatt ttagtcagag tttgggtgag aaaaggttga ttaagtagag
    21181 gcatttgtcg gttaagttag aatcggccct tagtctgcgt gtagtctagt aattcgcagc
    21241 gacacataaa tccaaaaccc tacaaatgcc atacgaatca accacaaact gacatttgtc
    21301 attgacttgc tttttgaaaa gtactatact cacttcatcg aggtaacgcc accatgcgct
    21361 tatttcatgg aaatacccag gcaagttgat taaacacacg cggatattcc cgcgtgtgtt
    21421 tttttatatt ctcaaaaaaa ggagatacga aaaatggatt ctggactgat aggcaaggtt
    21481 gaaaaagcaa aacgatatgc tgaagacagg caccgttttc actttaatca gtttgatctt
    21541 gattttcacg gtgataatag cgagcatcat gtatcctata acaatggcgt gtttaattgt
    21601 gattgcgaat ttttcatcac tcacaaacgc tgcggtcact caatggccct tgagatcctt
    21661 ctaaaagaca tgatcactga atcggtggct tcctaaaaaa ttgaataagc aactgccctc
    21721 cgccatggcg gagggcagtt tttttggatg atcccgagaa aattacccag ccttagggaa
    21781 ttctcattaa atcttaaact ttttggtgta caattttcct tatgaaatca caactttccc
    21841 gacgcgactt cttaaaactt ggagggctga ctctgggtgg gttggctttt accccctttt
    21901 tgcctggatt gaccgatttt gatgatggtt ttgttgtgcg cattgccaca gaccaaatgc
    21961 cagtacgctc cgagcccaaa gatggaagca ggatttacca gacctggtac cgcgatgacc
    22021 ttgtgcatgt ctatgaggaa gtgaccgctg aagaaccaaa gcataatcct gtttggtatc
    22081 gggtgtgggg tgggtatttg catcgcggac gtttacatcg cgtaaagacc gtttatcagg
    22141 ttccgatgtc aagcgttcct gaagggaaac gcgtactcac agaagccagt gttccataca
    22201 caaccccgta tcgttttaca aaggcacgcg gttgggaggc tctcaacccg cctatttatt
    22261 atgggtcggt gcattgggtg gatggtgtgg aagaaggccc caagatggct gattacacag
    22321 gcgcatggta tcgcatttac gacgagttag actcgaatgt gccgtattat gtccctgcga
    22381 tacatatgcg agttttacct tccagttact ttgagccgat ctcgcccaat gttccttatg
    22441 aagaaaaact catcgaggta aatctttcca cgcaaactct ggttgcgtat gaatatggaa
    22501 aatccgtttt tcagaccaat atctcatcgg gtattcccgg tggtggtttg ggcggtgata
    22561 aaggtatttc cacaacgacc cccaatggca aattcaccgt tttagacaag gtgcccgcca
    22621 agcatatggg ctacagttat ttcagcattg gcaagggtgg tgatgtcacc accgacgaaa
    22681 gtggttacgt tcttcctggc atcccatggt caacgttttt taccccggtt ggtcatgcat
    22741 tccatggcac ctattggcat gaaaacttcg gtcaggagat gagccatggc tgtatcaaca
    22801 tgcgctcaaa cgaagccaac tggatctttc gctgggtaca gcccaatcac agccccgaag
    22861 ccatctccaa tcatcgcggt cgcggtacta tggtggagat ccactactag ttttttgtgg
    22921 actttctcct ctcaaacaaa atcctcgcct cattatctaa tttctcaata tgcctattct
    22981 caactggaag ggaaagcatc ccgctgcgcc tgagcctgct tctctgattc aagatgccat
    23041 tctctacccg aaaggacatg gctatcccaa aacacgcccg tcagggcgtg ttattttggg
    23101 cgaaaatctt tcggttatgg cggccttact gcctgaatat gaaggccgca ttgacctgat
    23161 ttatgctgac ccgccgtttt ttaccaatag aaagttttct gcacgcattg gccgcggcga
    23221 agactcacgc aagcctcaca agtggaaact cgctgaaggt taccacgatg cctggcctga
    23281 cctcgattcg tatttgcagt ttttatacga acgcctggca ttgatggttc gtctgctttc
    23341 tccgaacggt accttgtacc tgcatctcga ttggcacgcg gatgcttacg cccgcctcat
    23401 tctcgatgaa ttatttgggt ccacgggggt ctttatcaac gagatcatct gggcctatca
    23461 cggaccatcc ccgatcaaaa cggcctttaa tcgaaaacac gataccatcc tttcttatgc
    23521 aaaaagcggc gattatacgt ttaatgtaga tgctgttcgt gagccatata atcccagcac
    23581 catcaacacc ttcaagtcat cgcggaaggc ggggtttggc aaagttccta atttggagcg
    23641 cggcaaagtt cccgaggatt ggtggtactt tccggtagtg gcgcgtttgc acaatgagcg
    23701 cacaggctac ccaactcaaa aacccgaagc gttacttgag cggattatcc ttgcttcgtc
    23761 caacaaaggt gatttggttg ccgatttttt ctgtggttca ggcacaacag ctttggtggc
    23821 tgcgaagcat ggacgaaagt tcattaccag tgatgaagct gtccgtgctg tgaatactgt
    23881 gcgaggcagg cttgtggaag cgaaatcggt ttttacggtg gaccgagacg aatccatcaa
    23941 aagtgttttt ttggacggtg ctaaaaaagt caaggcacat gtgctggaaa attcgattcg
    24001 tttggagacg tctctcgatg tggatttttg ggaggtggat cctgattggg atggaaaaac
    24061 cttcaaaagc gcagcgcagg ctcaccgtca tgcacgaagc ggcgaaatcc cccttgagtt
    24121 ggaaataaaa atcggaccca agcacaatgc ttgtatccga ttggtaacgg ttcagggaac
    24181 acaataccag ttatatatct agtcgatagc caacaccgcg aatcgttttg aggaattttg
    24241 gattgtcggg gtcaagttca atggcgcgac gcaaccagga gatgtgcaca tccagagtgc
    24301 gtgtgtcgcc ggtgtaattc gtttcccatg cttttttgaa aaggggttcc cgttcaacca
    24361 cttcgccatg tttatccatg agaaggtgga gcaaagtcac aagccgtggg gtgagttttg
    24421 tgcttttacc gaggcaacga acgcggcgtt tttccaaatc aagacgaatg gggccgacat
    24481 gcaaaacatt ttttccatcg ccaggcagga gcggtttaat acgattaaca agtttctgca
    24541 cggtgaaagg cagtcccaac accgcatcgg ctgcatcttt gctgacatca ccgtcatttt
    24601 caacaattaa gataatgggg agctttgcat ctttttcgcg aatcgcctga cagatgcgca
    24661 agcccgtgct gcgcaaagat gctgcgttga tcaccacaag gcttgggctg acatctttaa
    24721 gacgagccac tgctttgctg ccatttggtg caatttgtac atcaaatccc ttcttttgca
    24781 aatcaggtgc aaaggaaggg atctctgaat ggtgaacttc tatgaccaga agcgttgtgg
    24841 tcttcataat gaatgagtga atgatgcctg tgtttctgtt ggaatactta acgcaatcat
    24901 gtaaaatgtt tgcagtgatt ttgttaaggt ttcgttatta tacactaaat cttaacaaat
    24961 tgtttttatc caaaagtacc gcttcatagt aatttgatga gagttgcata aatattttgt
    25021 aatgagcagc ttgattgcat tgcttgactt ttgtatcgtt tgttggtaat atcggcgggc
    25081 tttactaaac gatgcggggt ggagcagtgg cagctcgtcg ggctcgaccg cgggagaagt
    25141 ttaacaaatg aatacgaaac ataaaggaga tattgcagag caggcagcag ttctagaagc
    25201 attgaagcga ggttggggcg ttcttcaccc agttggtgat aatctgccct acgatttggt
    25261 ttttgatgtt ttcggcgtgc tgattaaggt gcaagtcaaa tgtgcttggt ttgatgttcc
    25321 atctcaaaat tatgtagttg ataaccgtag aacgaagaca aataggcggt ccatgattag
    25381 agatgaatac actgaaacag actttgattt tgcccttgtg tatttgccgg attgcagttt
    25441 gttttacata tttcctgtaa acgtgttcat cagttacggt agcgaaatac atatgaccga
    25501 aactgacaag cgtcaaagaa gaccgaggtc ttttgaatat cgtgatgctt ggaaactaat
    25561 ccagcaatgg gctgcgaatg aggaaactca ttcgtgatca cttgtcaaat tcggggaagc
    25621 ctttcgcaaa ggtaatcccg agccaagccc tgaagaatat tcagggaagg tgtagagact
    25681 taatggcagg cacccaaaca gatactgctg tgggtgaaga gaaagtccag accacaaact
    25741 gacaatggtc aggcggcgaa agtcgaagtg gtatgcataa cccgaaggtc gcaggttcaa
    25801 atcctgcccc cgctactaaa taaaaagaga ccgttttaca cggtctcttt ttattttatt
    25861 cctaccattt acagtccggt gtggtgttga tgccagctga cttgccaaca cgtttgtaca
    25921 tggttacatt ggtgaaaggc tcatcaagca gaccatcatg aatggcccag gagcgtttgc
    25981 gaatcccgtt tgcggcttca gggtggcgga agggggtgga accgtcgagt ttggcggcga
    26041 gatcgcctcc attttgaaac cctgcgtgga tgctgtccat gaggcgtttg ccaacttgat
    26101 atgaaaaacc gtaacgttcg aagatgaccg cgttgtgata atacaaaggt tcaacaaaat
    26161 acatatcatg accaagggcc gcaacaaatc cttcgaaggc ttcgatggcc tgacctaaca
    26221 gccttaatcc gcgccgcacc tggcctgggg ccagacctgc ttcgagggct tctgtttctg
    26281 atgggatatt cctttgcagg gtgccgaatt tggtcgggga gccatctggc attctgtcta
    26341 cattgaagcg aggcgatgca ggatcgttga gaatgtagag caaaacatgg atctgcccat
    26401 tgagggtgtc ggtgaggtgg gcgtataaaa taggatcggg aaaatccacc tgatgaaaaa
    26461 ggcgcatttc cacatctgtt gagcccgtgg caaagcggaa ttgtaaaagg ttgtagcctg
    26521 ccgctgaggt gagcggaggg aggttgtatt tttccagaag tgcggttggg atgtaccgtg
    26581 aatagatggc ccgcttttct gattcaggca acaggttgat tccgccgatg gtggaggggg
    26641 gcatggtgta gtctccttgt tgctggtctt ttggtcttat agcctatgcg atagacgata
    26701 agtccagata ccgacaacta tctcactctg gtcgcgcgtt cgttgctgcg ggcttcatcg
    26761 cgtttggcga tggtggcacg tttatcgtat gcttttttac ctttggcaag ggcgatctcg
    26821 atctttgcac gtccgtcttt gaggtagacg cgggtgggga caacggtgtt gcctttgatg
    26881 cggacgttgt tccacagttc gcggatctgt tttttatgca ggagcaggcg gcgtttgcgt
    26941 ttcggttcgt ggttgaagta tttgcctgcc tgttcatagg gagcgatgtg cgcttcgaca
    27001 agccaggctt gttccccatc tgaaatgtcg acgtaggctt cctggatgct gacctgcccg
    27061 gcgcggacgg atttgatctc ggagccttgc aggacaatgc ctgcttcaaa cttttccatc
    27121 aggaagtatt cgaagctggc tttacggttg gttgcgatta ctttgatatc ttctgtcacg
    27181 ggttgatttt agcataagaa ggttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27301 nnnnagatta gccccatttg tggacggaga gacctgcgag gaggcggagg atgccgatga
    27361 agataagggc attggcgagg ccgatggttt cggcgatggc tggggcgagg aaggagcctg
    27421 caaggatggc tgtgtttaga ataatggtgt accacgcaag gtgggaggga cggtcattgg
    27481 gggggatgtt ttcgagcatg tagttgatgt aggcgccgtt gatcatggcg aagaggaagc
    27541 cgccgatgaa ggagaggcca tagaattgcc atatgtttcc tgaaaaagcc agtaacagcg
    27601 gatagatggc catgcccgcc gctccccaag ctgtgacctt tttgtttccg tagcggtggg
    27661 cgatgcggcg gaattgggtg gagccgatga ggacggtgag gtagtagatg gcagtgccaa
    27721 tgccgatgtt gttgtcgttg aggttgagga tgcggacgtt gtaaagcggg tagatgggcg
    27781 tggggaggaa gtgtgtgagg tgaaagaaga atagaacaag caggacgttg cggaatttgg
    27841 tgttccagat gtcgaggcgg agggtggtgt ggaggctgcg gggagagtct gtttggggtg
    27901 ttggggcagg tgtcggtatg gcggggagcg gggcggtctc ttccaggggt gtgacgtgat
    27961 aaatgtggta gctgctcatc gctgcgccga atgctccaat cgcaaagatg acttgatagc
    28021 ccgtttcaaa gggtagattt ttcaggatgg cgcctgcaat cagggaggtg agcatgtagg
    28081 cgatggcgaa ggtgacgttg cgtgtgcctg ccacctgggc gcggaatcgt tcggggacgg
    28141 attcggcgaa gagggcattg aagccgacgc cgagcggggt gagcggaatg gccatgagga
    28201 aggcgaggat gatgagcgcc cagatctggc cttgttcatc gaagagccag gggaggggga
    28261 tccagaaaag gtagccgata cgaaagagga cggaggacca gaatacggcg cggcctgtgt
    28321 gtcttttttc gatccagcgg cctgcgggga tggcgagaaa aaggttgacg acggctgcca
    28381 tggcggcgag caggccgatc tgaaaactcg ttgcgccaag acgggtggcg taaacgttga
    28441 ggaagttgac cgatgtgccg ctgaggacgc cgaaccaggc gatatcaagg tagaggttgg
    28501 taaagttggc gcggtatttt tgcggggtgt cggattgttg aaagaagttg aaacgcata
//
LOCUS       DAOQLC010000051        28419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_531_length_28419_cov_21.630518, whole genome shotgun sequence.
ACCESSION   DAOQLC010000051 DAOQLC010000000
VERSION     DAOQLC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_531_length_28419_cov_21.630518"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PL000_10580"
     CDS             complement(<1..1167)
                     /locus_tag="PL000_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89391.1"
                     /translation="MRFAELFFFGFTLWIGSYLLARQSQKITVQLTGWGLLAYALALA
                     IQILFDQSILVILLIPAMLWIGAALHLLPEENPLRQIFIRTWAVTSIPVVILTLVNAW
                     FAAVIVLALVICAGMVAQMAARSQFKNTFALLGVIGLFVTLSVGLLILPLNWIPTSWG
                     MVLLGFDLVLLGITITAWDAFDEGESIRAHLLRSFVSNLYYSGALVILASLFNGSDIL
                     LIALIAFGILTQSFSNALQSVLDRLTLPRQTTDERQTLRQTADALPLLTALKTDSVTE
                     EQFTRLTRRALSHLGDLPKLATNPLTNLPQIKESNPLERAHALKSLLVQSIQKLKPQS
                     EAKFGTTDEWRYFNAVYFPYVLGLKPYTRRADLDSLDEESRAALDWFQTSVPERT"
     gene            complement(1178..1768)
                     /locus_tag="PL000_10585"
     CDS             complement(1178..1768)
                     /locus_tag="PL000_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC89392.1"
                     /translation="MELKDTVLKRRMVRNFADTPVSPEIIERIVDLTRHAPSAGFTQG
                     QSFIIVTQPDMRKAIADTCEEGEYVKSGFDPFISKAPVLLIPCTSELAYHRRYQEADK
                     VDDKGNEIVWPVPYWFMDIGCAVMIALLATIDEGLVSAFVGSKDLKTLRTLLSIPEEV
                     TPVGVIAVGYRAPDRPSPSLKRGRKPDQDYIHREKW"
     gene            1921..2739
                     /locus_tag="PL000_10590"
     CDS             1921..2739
                     /locus_tag="PL000_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89393.1"
                     /translation="MSKTKVDILTLIAVSAFSFMFATAIHEHVGHSLACISVNGRLSE
                     MGAFYADCAADSVSDLGGRFVALAGPLVSLAAGMFFLRLFGKMFSKAGAVLKFFLWHQ
                     ATVNLMIAAGYLLFSGVAGIGDLGTVSGGVFYQAEPEWLYRAALTVFGLAGYYAVIRI
                     SIQKMDAFIGGEGVERVNRAQMTSLIAYFSGGAVALLIGLLNPHGIFIVLAASVASSM
                     GGTSGMAWMMQLLDRKKNTGDAPFTLQRNWGWILGSGAFLLIYAAVFGPTIFLK"
     gene            2795..2962
                     /locus_tag="PL000_10595"
     CDS             2795..2962
                     /locus_tag="PL000_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89394.1"
                     /translation="MYRTTVAMYLAAFGIIFFFAMPFKVLEDNVATFFGIAFLILSAV
                     TWGFWPTPKKQ"
     assembly_gap    3072..3171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3172..3852
                     /locus_tag="PL000_10600"
     CDS             <3172..3852
                     /locus_tag="PL000_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC89395.1"
                     /translation="KSIKRNLGLLASLFALAVLSACTAATPIATPTATLIEPTATQVV
                     PTATLVVPSTSPSVTSDKKALLKELTKAIRDHNAEEVKRLLALGMDVNEIQSFRQLTP
                     LALASYEGDTEIIALLLEHGADIAMYNKNQYGTTALIEAAQNGKTDAVKFLLEHGADI
                     NQRDKFGDPALNWATYYGRVDVVKLLIENGADLTVVGSGGGTALNTAIAQGHEDIEKM
                     LRDAGATR"
     gene            3890..5074
                     /locus_tag="PL000_10605"
     CDS             3890..5074
                     /locus_tag="PL000_10605"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC89396.1"
                     /translation="MMTKKSEIEKPHVWDRWDWIFHTISYGVLIFQAAIFLSNEVKHA
                     SLQIFFGLTAAVMVWYFPLVFKRQALWMQIPARTLAYFVIGWFLWGTLILAHPAAMML
                     AAMYYPILFMRMPIRWAAASASLLTFGLLAASIFVLGLDENLLPTLVTITGLILGGAI
                     VIGVFINSLISQSVDRQRLIEELSQTRASLLKMEREAGVLAERQRLAREIHDTLAQNF
                     TSIIMHLTAAKLGDSESAVPHVQQAEQTARDGLDEARRIVWAMRPEQLEHSTLVESIE
                     VLAARWSAENSVRVNTAITGTPRPLGPERDAALLRVTQEALNNVKKHANASRVDITLS
                     YMADLTALDIVDDGIGFEEKDQHGFGLKTMRERVEELRGTLTIESSDGTAIAVSLPVE
                     DE"
     gene            5071..5700
                     /locus_tag="PL000_10610"
     CDS             5071..5700
                     /locus_tag="PL000_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89397.1"
                     /translation="MMIRLLIADDHPVVRSGLRAILASQPDFELLAEADNGFSAVESA
                     AKFQPDVILMDLQMPAMDGVSAIAKIHAANPSIYILVLTTYDTDVDILRALDAGATGY
                     LLKDAPPDELFRAIRSAARGEVTLAPSIAAKLTRRLTHPLERSLSGREIDVLSLAARG
                     DSNKEIAIKLHITEATVKSHFVHIFNKLNVTDRTAAVTMALEKKMIRLE"
     gene            complement(5708..6286)
                     /locus_tag="PL000_10615"
     CDS             complement(5708..6286)
                     /locus_tag="PL000_10615"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC89398.1"
                     /translation="MKVNINAKSINQVVNAETVNIDQRSGESESPPSDESASGGIFIS
                     YRRKDSADVVGRIYDQLVKHFGAQHVFKDVYSIPLGSDFGKYIIEAIGRSGALLAVIG
                     EKWLETNDAGKTRLDEPNDFVRIEIESALVNEIPIIPLLVGGATMPPKEKLPPSLEKL
                     SEKNGIQIRPDPDFHMDMKRLITSLEKHINTH"
     gene            complement(6296..7921)
                     /locus_tag="PL000_10620"
     CDS             complement(6296..7921)
                     /locus_tag="PL000_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89399.1"
                     /translation="MSATIPETLIADHTAPDCPNQLRQNVTKHLTYHIAASIISKCDF
                     PLIESLPKPFAPPFNSLGHYVLFDYKLLVKIIWQVIGEIKSSSYKVYKSILQKSLGFG
                     ALTAVSAFLLLGPTSQPEILILALLTGGLVFFIYKSYGSLKILPKTVSDIRARAIAQL
                     VAGNWTAKPDPNIDRKIENEFKLKEIGDGIIGGKKIPIIVITNSLHPFPGYGWLQAQN
                     VFVCKPKEQTTPSGSIHEIIEKVQENIHGKIAEFPLKEVHCGNLIAIHGDSLFMDSAW
                     LNKDKSPHLWADMHTLKNLNSIDSRASARCYFVIQSFFPEHDTVANFFVRPFMAGNSI
                     SCQIALTTIGPLIHRNDYFQERLLQHRREQEDAPISATALPTPLLEFQNSAQKTNLLK
                     QSIKQSSKKFRSNKMHYAEIKKLNPIETEDEQKNFSKELKELMEVTTTWPGRLTPLEN
                     WRESYSLTFTNDFFGSTEALASIKTLYQQISRTILDTFDSMGFDITDYRDREGKYSIN
                     AEKIDRLIIGQEIKILQTADARLPAKPQENSIS"
     gene            complement(8030..8299)
                     /locus_tag="PL000_10625"
     CDS             complement(8030..8299)
                     /locus_tag="PL000_10625"
                     /inference="COORDINATES: protein motif:HMM:NF039404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HNC89400.1"
                     /translation="MCTMIVEKVKIEGSGKGTQGWFPLQQANVSYDHPFDAPYEHALN
                     IDFVNEAQGPSARVAVELSESAARELVRTILAVLDQAESGGHLVK"
     gene            complement(8455..9210)
                     /locus_tag="PL000_10630"
     CDS             complement(8455..9210)
                     /locus_tag="PL000_10630"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC89401.1"
                     /translation="MYTTDMYEGVLAETVTMTGANGDKINAYLAKPLGKGPFPAMVLV
                     HHMPGWDQWYREATFKFAHHGYVTLSPNLYFRSGHGTPEDVAAKVRADGGVPDEQVIG
                     DLTGAMNHLRGLAEVNGKVGIFGTCSGGRHAYLAACRTTGFDAIVDCWGGRVVMTPDQ
                     LNANFPVSPLDYTKDLSCPILGLFGEDDSSPTPEQVKIHEAELKKYGKGYEFHMYPNA
                     GHGFFYYDRPNYRQEQAVDGWKKTFAFLEKHLA"
     gene            9408..9680
                     /locus_tag="PL000_10635"
     CDS             9408..9680
                     /locus_tag="PL000_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89402.1"
                     /translation="MEIAVGRVTHYFDHLKVAVLALNGDLKLGDKIHIVGHGTDLFQR
                     VSSMQIEHRSVVWVKPGDHVALKVIEPVHEHDAVFRIIEEVLEPLV"
     gene            complement(9810..10199)
                     /locus_tag="PL000_10640"
     CDS             complement(9810..10199)
                     /locus_tag="PL000_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89403.1"
                     /translation="MTFTYVDPREREDIRAHQGEDVWVLLNHVKADKCGEYEHFLHEI
                     LKPAIAHIHPETYNKVRVLHPSLPNKDGTYTYIFLMDPVVADGIYDIGEILHSFYRPE
                     LADQYMKIWNNSLASPQIEHDMIQSSW"
     gene            complement(10386..11084)
                     /locus_tag="PL000_10645"
     CDS             complement(10386..11084)
                     /locus_tag="PL000_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNC89404.1"
                     /translation="MTTQKTNLDPLISSPDSNEYFNSISHLIGAILSISALTVLVSLA
                     AIADKPIHVVGFAIYGVSLFLSFLFSCLLHFFLLFGKYLRVFGILDHNAIYILIAGTY
                     TPFCLTIFSGATGWVLFGVIWSLAVFFITLKSIFFTKISVLMSNISFLLMGWIIVFLI
                     GPIYTQLGMGAISLLITGGLFYTVGALIFAYGKPNPFPPYFGNHEIWHLCVLAGNTTF
                     FFVMLFYVLPFPTS"
     gene            complement(11081..11536)
                     /locus_tag="PL000_10650"
     CDS             complement(11081..11536)
                     /locus_tag="PL000_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89405.1"
                     /translation="MTYLPGILMRGHQVASRPSKDYPYSSLEKQKPYFKALGLDLAPY
                     FNGTLNISIAPLEFEMTRPEFTFPLVEWTDLHPPETFSFSRCRVRFEGKEYAGWVYYP
                     HPETKKTHFQNPSLVEVITYEIAGIQYGDALEIEVNPQEITITGYTPAP"
     gene            11869..14586
                     /gene="alaS"
                     /locus_tag="PL000_10655"
     CDS             11869..14586
                     /gene="alaS"
                     /locus_tag="PL000_10655"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC89406.1"
                     /translation="MSKKLTGNQIRQDFIDFFVEHGHTAVPSMSLVPGGDATLLFTNS
                     GMVQFKDVFIGTDTKPYTRAVDSQKCMRVAGKHNDLEDVGRDDTHHTFFEMLGNWSFG
                     DYYKKDAIAWSWQLLTEVWGLPKDKLYATCFRDDKGNVPQDDEAADIWKQQPGFDPEH
                     VLFFGRKENFWQMAETGPCGPCSEIHIDLGDERDNMRGTDHVCGVNGECTRYLELWNN
                     VFIQYNLFDDGRLELLPAKHVDTGMGFERIVSVLQGVDSNYKTDLFTGSLEVLRALTG
                     HTEKEMLANFTPYRVICDHVRSASFLIADGVVPGNAGRNYVARMIVRRAARFGTKIGL
                     TEPFLAKVAEAVIEQYGDFYPELRKTQPAILDNLTREEIRFARTVEAGTAHLENLLSE
                     LRSTNQTVLDGHKAFDLYATYGLPFEISRDIAREQGLDVDEKGFTTAKEEHAIASGGG
                     KAMGKLGGEDAEFFAGILKDLQAKGKLSKDGVEYDPYNGLDVSRYSNLEILALVVNGQ
                     SVDSANLDDQVEVILPKTGFYIEAGGQVNDTGFIRGEGWEIEVTGIRRASAGVIVHIG
                     EVISGHPKVGDKATAEVDANRRHDIMRNHTATHLLHKALHEVLSDQAKQAGSLVSPTH
                     LRFDFNHPEAMTPDQVERVERMVNEAVAMDMPVVKVTKSLDEAKKEGAMALFGEKYGE
                     TVRTISIMEGGEDKYSYELCGGTHLERTSDVGAFIIVSESSAAAGVRRIEAVTGRGAY
                     DLIHKRFKTLKQAAGSLKAAVEEVPAKVDALQDEVSELKKELANLRTQSALSAFNLQL
                     SNVQTVKDVSVLAAEIVNADTDTLRSLADKFREKYPKAGAAVLVTGTTVIAVVTEDLV
                     KRGLKAGDLITGIGGKGGGRPNLAQGSLPDGNINESLGKLAKVVEEKVK"
     gene            complement(14652..15251)
                     /locus_tag="PL000_10660"
     CDS             complement(14652..15251)
                     /locus_tag="PL000_10660"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC89407.1"
                     /translation="MSNANKQKYPGKRQKRRNSSTAMGIGLILFGVAFFLWLLGSSGK
                     ASLPTADEISIVPAEVNFPAPQLALENLNGEKQTLEDYQGRIVLVNNWATWCPPCKAE
                     MPILKQYHDTHAAEGFTVIAVEAGDAKDTVAEFAKSLGLPFPVWLDPESASINAIHNG
                     SLPNSYVIDRTGTVRYAWTGEVTMTMLEKYITPLIAENN"
     gene            complement(15268..15477)
                     /locus_tag="PL000_10665"
                     /pseudo
     CDS             complement(15268..15477)
                     /locus_tag="PL000_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(15493..15936)
                     /locus_tag="PL000_10670"
     CDS             complement(15493..15936)
                     /locus_tag="PL000_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89408.1"
                     /translation="MDKLLNEQVVGQIKQAFAAITNPVQILFFGSKDNCEYCGETGQL
                     VKEISEIDDRVHLSTHDLHEDAELAAQFRVDKAPVIVIAGRDGDTVVDLGIQFSGIPS
                     GYEFSTLINDILFASKRDSGLGAATREFLKSLTKPLHLQVFVTPT"
     gene            16053..>16581
                     /locus_tag="PL000_10675"
     CDS             16053..>16581
                     /locus_tag="PL000_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC89409.1"
                     /translation="MSITTTPEISIEALRSGSRAEFARMVDVHSPLIYRLGLKMLGNP
                     QDAEDVLQNTFLNALTHLPDFEGRSSVSTWLYRIASNEALMMLRKKRPLNIEDLQAEN
                     SDEGMVPEIFVDWSALPEGELLAGESKNVIDDAVAKLPEQLRIVFVLRDIEGISIRET
                     AEILSLTETNVKTRLL"
     assembly_gap    16582..16681
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16815..17078
                     /locus_tag="PL000_10680"
     CDS             16815..17078
                     /locus_tag="PL000_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC89410.1"
                     /translation="MNHPLHDNCKALLGSLSEYIDGELPSDLCREIEKHLEGCDNCRV
                     VLNTTKRTIDLVQIPVKEESVPDDVRERLFKRLKLDDYLNPQP"
     gene            complement(17095..18696)
                     /locus_tag="PL000_10685"
     CDS             complement(17095..18696)
                     /locus_tag="PL000_10685"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNC89411.1"
                     /translation="MDKPWYHKTSIYQIYPRSFHDTNGDGIGDLKGIIQKLDYLHELG
                     FETLWISPFFSSPQADTGYDISSYTDIAPEYGSMEDALQLIEGVHQRGMKIVFDMVMN
                     HTSDEHPWFRESRSSQDNPKANWYIWQDKPNNWQSMTGGSGWHYAPERNQYFWSSFLP
                     FQPDLNYRNPEVKRAMLEIVRFWLGKGVDGFRLDIFNVIYKDAAFRDNPFTFKLMPTE
                     DDPSGFFQEAKYNLNQAESFEFARELRSVCEEFGERMLLGEVSGNKSTLRKFLGEETN
                     NGLGLVFDFEMLSFQFTAEYFRELLERIEAHFPAPFMPVYVFSNHDRRRSILRLGNDL
                     RKAKLLAMLQLTTRGVPCMYYGEEIGMTDLPQPFGAARDPIPHKFKLIPRFIFNWLNL
                     TINRDEVRTPMQWDGTGNAGFSSSEQTWLPVHKNHLEINVEKANQDESSLLNIIRKLM
                     KIRKNETVLQEGSLVVLKNLPHGILGYRRTWDHTSLTILLNFDGSEKVVRTEFSECIF
                     ALSARADLKEKAIHMNGFGGVILRA"
     gene            complement(18701..19990)
                     /locus_tag="PL000_10690"
     CDS             complement(18701..19990)
                     /locus_tag="PL000_10690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC89412.1"
                     /translation="MIQNTCLWWETAPTPASYKNKPLPAKTDVVVVGGGFTGTSAALQ
                     LAKGGARVTLLEAKTIGFEASSRNGGQALSCLHHTLTNAIKEHGRELAKNMFLAATYA
                     ANTVEQIVTEEGLDCDFVRCGNIEAAFKPAHFEKLKREQETLHEVAGYEVQVLPRNET
                     RTELGTDSYNGMMINPCSGSVQPAKFVRGMAMAAERAGADIHEGTRVIVIERISDSPA
                     SDGSRFLVKTERGDILTKEIMLAANAWIGDILPQFRRRVLPVESYIIATEPLSEDLAK
                     KLIPNNRVVYDTRKTLAYYRLSPDNRMVWGGELTFANTSQRTNIHTLQRGMNKVFPEL
                     ANHKIDYFWNGTMGITFDENAHAGQIDGMWYSMCYVGHGVTLGTYLGRQMANGILGKP
                     SNNPFADLPIPYASPFQENSWVVDAGKLWFRFLDIFQ"
     gene            20288..21889
                     /locus_tag="PL000_10695"
     CDS             20288..21889
                     /locus_tag="PL000_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HNC89413.1"
                     /translation="MKPLRKAIGFSVLLLLLFTSACGGSAPAATEAPAATEAPASPDY
                     SFDAPAATEAPAAEAPAAIPQQQNGQQPPATGNDPYDMFFEDYGVNPSIDTEDDNLST
                     FALDVDTGSYTVMRNYLQDGNLPPQDSVRVEEYVNYFDHGYPTPPPHQAFGIYVDGGP
                     SPFTETERYDMLRIGIQGYEVADEDRKDAALTFVIDVSGSMDMDNRLGLVKHSLELLV
                     EQLHRNDTVSIVVYGSEARVVLEPTRGSDSGRILDAIYSLSPEGSTNAEAGLRLGYQM
                     AMEAFMRDGINRVILCSDGVANVGLTGPDAILGEINHYVEEGVTLTTIGFGMDNYNDT
                     LMEQLADNGDGFYAYVDDIDEARKLFIDQITGTLQTIALDAKVQVDFNPQVVMRYRLV
                     GYENRAVADDEFRDNTVDAGEIGAGHSVTALYEVKLYPEARGRIATVFMRWEDPDTHQ
                     VVEISKDFETSQMAYDFRETDPYFQRAVVVAEFAEILKDSYWAEESSLDDVYDEARRI
                     ADYLYRDDVMDEFVDLVREARRLMD"
     gene            complement(21976..23676)
                     /locus_tag="PL000_10700"
     CDS             complement(21976..23676)
                     /locus_tag="PL000_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC89414.1"
                     /translation="MKLKMFTLLVVFGLVLSACSGGGNAAATEPASSGGGTVVLILPE
                     EPTTLNFYLADAAIVRQVADATSMTGLVTIDETGNFVPTLAQEVPTTENGGLSSDYLT
                     VTWKLKEGLKWSDGEALTSDDVKFTVEVLSNPDSGALVGTSGFDLITKVETPDATTTV
                     LTYSEPYPGYLDQFAYGLFPRHATGKPEEMSAWAWNSNPVVAGPFILSNWESGKRLSF
                     TRNPNYYEAGKPYLDEVVFEVVPEPAAQTAMMLNGEAQMQLWPSEFKADYDTLLTGKA
                     QQYLIPGIWNMAIDFNLSAPFDGDPTASAPHPILGDIRVRQAIAHAINYQSLQQDVLK
                     GSVSDSTNPFAYGWYKCELPRQFGYDVEAANKLLDEAGWVMGDDGIRVAKGAKYAEDG
                     TRLSLELQGYTAFDPLQLTEEFIVENLKAVGIEARIQNYDFSIIFGTFEDNSPRAVGD
                     YDMLIFDRGFTTEPQGYNFDAYHSTRIPTAENPTGGNYFRWINAEVDSALDTAGSSFD
                     IQTRKDAYCKVGQAVLDELPQVYLYLFQDNYGIADSLSGYTLNTWGSMSWGIQNWKFK
                     "
     gene            complement(23711..24607)
                     /locus_tag="PL000_10705"
     CDS             complement(23711..24607)
                     /locus_tag="PL000_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89415.1"
                     /translation="MSDISLPREQSMGRIVLRRFFKHKLAGIAIGILLILILVAVFAG
                     LNSHNPLEQNPTNSFATPNATNWFGTDELGRDVLSRIMYGGRVSLAVGLLSTLLSILL
                     GITVGALSGYFGGWLDSTLMRVTDAFLTFPTIFVLIILGAFLREQQLFILQNSIFVVI
                     IIIAALSWMWPARLVRGLFLVLREKEFVTASRALGGSDFRIIVQHILPNCMGPILVSG
                     TLQMASAIITESGLSYLGFGVQPPTPTWGSILSTAQNQIFRAPWLGFFPGFMIFITVM
                     AINYIGDGLRDAFDPYVIHSEK"
     gene            complement(24600..25574)
                     /locus_tag="PL000_10710"
     CDS             complement(24600..25574)
                     /locus_tag="PL000_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89416.1"
                     /translation="MQTYALRRILQTIPILFIISVLLFFLVRSAPGGPLTAARRNPNI
                     SKEQIEALEEKLGLNDPLPVQYGRWLVDLFQGNLGESIKFRRPVSEMIGERIPNTLLL
                     VGVSFLTTLMLAIPIGILSARKPYSLFDYTMTTITFIGQSIPVYWLGLGLIVVFYVTL
                     KNPFTGDPLFPVGGMNTRGQEGELLDTVWHLVLPVIALSLGWIAWYSRFLRSSMLDVL
                     HEDYVRTARAKGLKDNAVYYKHALQNAILPLVTLIALDLPSLFAGALFVETIFSWPGM
                     GRLFWDAAKGRDYPVLLGVVMITAVLIIVCNILADLAYGWLDPQVKYE"
     gene            complement(25577..27166)
                     /locus_tag="PL000_10715"
     CDS             complement(25577..27166)
                     /locus_tag="PL000_10715"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNC89417.1"
                     /translation="MSTKADLVIENAKIFTSNEAKPYAEALAVKGNRIVFVGTNEAAK
                     PYKDESTKVIDGQGRTVTPGFIDSHFHLLWGSIWAGSAQLYEVKDLNDLKTVLQNFAT
                     ENKTAAWVDGRGIKYNIVQNRQQLDEIVPDRPVYINAYDGHTSWANTKALEMAGILQP
                     GVDAPGNGVIVRDENGMATGELRETAMNMVSDLIPEPDEVRKRELLKLTIQRINATGV
                     TSVHNMNGDMEEMMVYAALEDANELTVRVYVPYWVKPETTEEMLKEAAEMANVQGEFA
                     RGGAAKFFMDGVWESYTALNLEPYADDTEAAAEGIYSLEHFTRMAALCDKMGLQIFVH
                     CCGDGAVRRVLDGYEAVQRSNGKRDSRHRIEHIEVIHPDDIPRFTQLGVLPSMQTSHA
                     PFSISEGDVWPTRVGEKRWPLSFAWRNMKNAGNNIAFGSDWPVAPYDPMINLHVGLNR
                     EKWNPSDPDQTLSLEELIIGYTRDAAYAEFKENEKGQIKEGYLADLVLFSHDLFSLPK
                     EEIMTAKPAMTIVDGKIVFEA"
     gene            27253..28323
                     /gene="mnmA"
                     /locus_tag="PL000_10720"
     CDS             27253..28323
                     /gene="mnmA"
                     /locus_tag="PL000_10720"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC89418.1"
                     /translation="MAKEKVVVAMSGGVDSSVAAALLKQQGYDVTGMMLRLWSEPGKE
                     DSNRCCTPDSMAQARRVAGILDIPFYVVDAKEVFRETVVQYFLDGYERGETPNPCLMC
                     NRQIRWTFLLNHALALGAEFMATGHYVRRRSGDDGRTELLRAVDHAKDQSYVLHVLKQ
                     EQLAHALFPVGEYPKPEIRRIAADFGLPTASRADSQDLCFLAGEDYRNFLQRNAAGML
                     KPGNIETPDGRIIGEHTGLANYTIGQRKGLGVASPVPLYVMTKRAASNTLIVGESHEL
                     GSTELTVRDVNWLSGEAPQEPFRAEVKTRYTAKEVTALVFPLEEKQAAVKFDAPVRDI
                     TAGQAAVFYQGEVMLGGGIITQ"
BASE COUNT         6558 a         6965 c         7559 g         7137 t
ORIGIN      
        1 tgtgcgttcg ggcacggagg tttggaacca atcaagcgcg gcgcgggatt cttcatcgag
       61 cgagtcgaga tcggcgcggc gggtataggg tttgaggccg agcacgtagg gaaaataaac
      121 cgcgttgaaa taccgccatt catcggtggt cccaaatttt gcttctgatt gcggtttgag
      181 tttttgaatg ctttgcacca gcagtgattt caacgcatgt gcccgttcga gcggattcga
      241 ttctttgatt tgcggcagat tcgtcagcgg attggttgcc agctttggca gatctcccag
      301 atgtgagagt gcgcgtcgcg tcagacgggt gaactgctcc tcggtaaccg aatcagtttt
      361 cagcgccgtg agaagcggca gcgcatccgc agtttgtcgc agggtctggc gttcgtctgt
      421 ggtttggcgg ggcagggtca accgatccag cacggattga agcgcattgg aaaaactctg
      481 cgtgagaatc ccaaaagcga tcagggcgat caaaagaatg tcgctgccgt tgaatagact
      541 tgcaaggatg acgagcgcgc ctgagtaata aagattggaa acgaaggaac gcaaaagatg
      601 cgcgcgaatg gattcgcctt cgtcgaaggc gtcccacgcg gtgatggtga tgccgagcag
      661 gacgagatcg aaaccaagca acaccatgcc ccatgaagtc ggaatccagt taagcggcag
      721 gatgagtaaa cccacgctga gtgtgacgaa taacccgatc accccgagca gtgcaaacgt
      781 atttttgaat tgtgacctgg ccgccatttg cgcaaccatg cctgcgcaaa tgaccagggc
      841 aaggacaatg accgccgcaa accaagcatt gaccagtgtg aggatcacaa cagggatgga
      901 ggtcactgcc catgtgcgga tgaagatctg ccgcagtgga ttttcttctg gtaacaggtg
      961 cagcgctgcg ccgatccaca gcatggctgg gatgagcaga atgacgagga tggattggtc
     1021 aaagaggatc tggatggcaa gcgcgagcgc gtacgccagc aagccccagc ccgtcagttg
     1081 gacggtgatc ttttgcgatt gccgtgccag caaatacgaa ccgatccaaa gcgtgaagcc
     1141 gaaaaagaaa agttctgcga agcgcatgaa ggctggttta ccacttctcg cggtgaatgt
     1201 aatcctgatc gggtttccgt ccgcgtttga gggatggtga gggtcggtca ggtgcgcgat
     1261 agcccacggc gatgacgccg acgggtgtca cttcttcagg gatggatagc aatgttcgca
     1321 gcgttttcag atctttggag ccgacaaaag cgctgaccaa tccttcgtca atggtggcga
     1381 gcagggcgat catcacggcg cagccgatat ccatgaacca gtagggcaca ggccagacga
     1441 tctcgttgcc tttgtcgtcc accttgtctg cttcttgata gcggcggtga taggccaact
     1501 cactggtgca ggggattaac aacacgggcg ctttcgagat gaatgggtcg aatccgcttt
     1561 tgacgtattc gccctcttcg caggtgtcgg cgatggcttt tctcatgtca ggctgggtga
     1621 cgatgatgaa ggattgtccc tgtgtgaagc ctgcgctggg cgcatggcgt gtgagatcaa
     1681 cgatgcgttc gataatttcg ggagatacgg gtgtatcagc aaaattgcgc accatgcggc
     1741 gcttgaggac ggtatctttg agttccatgt tttttctcca caattgattt gcgatgcgtc
     1801 attatagcaa agcgccccat ccatcgaaag atggatgggg aaatccatct gtggagagat
     1861 gaccatttga tgcggcccgc ctatactgcc agcattctaa tttgatggat ggagcgtttt
     1921 atgtctaaaa caaaagtgga tattctgacc ctgatcgccg tctcggcttt ttccttcatg
     1981 tttgccacgg ccattcatga gcatgtgggg cactcgctgg cttgtatctc ggtcaatggg
     2041 cgcctgagcg agatgggtgc tttttatgca gattgtgcag ccgattcggt ttcagatctg
     2101 ggcgggcgtt ttgtggcgct ggcgggtccg ttggtgagtt tggcggctgg aatgtttttc
     2161 ctgaggcttt tcggaaaaat gttttcgaag gcgggcgctg tactcaaatt ctttttgtgg
     2221 catcaggcca cagtcaattt gatgatcgca gcagggtacc ttttgttttc aggtgtagca
     2281 gggatcggcg accttggaac cgtgagcggt ggtgtgtttt atcaagccga accagagtgg
     2341 ctgtatcgtg cggctttgac cgtgttcggg ctggcaggat attacgcagt gattcgaatc
     2401 tccattcaaa aaatggatgc cttcatcggc ggcgaaggtg tggagcgggt caaccgcgcg
     2461 cagatgacct cactgattgc atatttttca ggcggtgctg tggccttgct gattggtctg
     2521 ctcaacccgc acggcatctt cattgttctg gctgcttctg tggcatccag catgggcggc
     2581 acctcaggta tggcgtggat gatgcaattg cttgaccgca aaaagaacac gggcgatgcc
     2641 cctttcaccc tgcaaaggaa ttggggttgg atactcggca gcggggcttt tttactcatc
     2701 tacgctgctg tctttggccc gaccatcttt ctaaaatagc ctaaagtgaa acaacgaaaa
     2761 ccgttcaaat aacgatcatt caaggagtat gaaaatgtat agaacaactg ttgccatgta
     2821 tctcgccgcc tttgggatca tttttttctt tgccatgccc ttcaaagttc ttgaagacaa
     2881 tgtcgcgact tttttcggaa tcgcctttct aattctctct gctgttacat ggggattttg
     2941 gcccacgccg aagaaacaat aaccatttca cacccgcacc gattggagac actctatgct
     3001 gcctttcctg aaaacaaaat ccatcaaacg aaatttgggc cttttggcat cccttttcgc
     3061 tttagccgta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaatccatc
     3181 aaacgaaatt tgggcctttt ggcatccctt ttcgctttag ccgtattgtc ggcatgtaca
     3241 gccgcaacgc cgattgctac ccccaccgcc acgttgattg agcccactgc aacgcaagtt
     3301 gtgcccaccg ccacactggt cgtgcccagt acatcgcctt cggtgacttc tgataaaaag
     3361 gcacttctga aggaattaac caaagccatc cgcgaccata atgcagagga ggtgaaacgt
     3421 ctgcttgcgt tggggatgga tgtgaacgag atccagtctt tcaggcagct tacaccattg
     3481 gctctggcat cttacgaagg cgatacagag atcatcgctt tgttgttgga gcatggtgcc
     3541 gatattgcca tgtacaacaa aaaccaatac ggcaccactg cattgatcga agccgcacaa
     3601 aacggcaaaa ccgatgctgt aaaattcctg ctggaacacg gcgccgacat taatcagcgc
     3661 gataaattcg gtgacccagc cttgaattgg gcaacctatt acgggcgtgt ggatgtggtc
     3721 aagttgctga tcgaaaatgg cgcagacttg accgtggttg ggtcgggcgg gggcacggcg
     3781 ttgaataccg ccatcgcaca gggacatgaa gatattgaaa aaatgctgcg tgatgctggc
     3841 gcaaccagat aatcctccgt ccgcagtttg ttgaacgagt atacttttga tgatgacgaa
     3901 gaaatcggag atcgaaaaac cgcacgtatg ggatcgctgg gattggatat ttcacaccat
     3961 ttcctacggc gttctgattt ttcaggcggc cattttcctc agcaatgaag tgaaacatgc
     4021 ctcacttcaa atcttttttg gcctgactgc tgccgtcatg gtctggtatt ttccgcttgt
     4081 cttcaaacgt caagcattgt ggatgcagat cccagcacgt acgctggcat atttcgtcat
     4141 cggctggttt ctttggggaa cccttatcct tgcccatcca gcggcgatga tgctggccgc
     4201 gatgtattac ccgatcctgt tcatgcggat gccgatccgc tgggcggccg ccagcgcttc
     4261 gctcttaacc tttggcctgc ttgcagcttc gatcttcgtg ctcggcctcg atgaaaacct
     4321 gttgccaacc cttgtcacca tcactgggtt gattctgggc ggcgcaattg tgatcggggt
     4381 ttttatcaac tcactcatca gccaaagcgt ggaccgtcag cgtttgatcg aggaactgtc
     4441 tcaaacccgt gccagtctgt tgaaaatgga aagggaagcg ggggtgttgg cggagcgtca
     4501 gcgtttggcg cgggaaattc atgatacgct cgcgcagaac ttcaccagca tcatcatgca
     4561 tctcactgcc gcgaaacttg gcgattcaga atcggctgtg cctcacgttc aacaagccga
     4621 acagaccgcg cgcgatggcc tcgatgaagc gcggcgcatt gtgtgggcca tgcgccccga
     4681 acagcttgaa cattccacgc tggttgaatc cattgaagtg ttggccgcgc gctggtcggc
     4741 ggaaaactct gtccgcgtga acactgccat tactggcaca cccaggcccc tcggtccgga
     4801 aagagacgct gcccttttgc gagtgacaca agaggcgttg aacaatgtca aaaaacatgc
     4861 caatgccagc cgcgtagaca tcacactttc gtatatggca gatctgaccg ccctggatat
     4921 tgtggatgat ggaatcggtt tcgaagagaa agaccagcat ggatttggtt tgaagaccat
     4981 gcgcgaacgc gtggaagagt tgcgtggtac attgacgatc gaaagcagcg atggcacagc
     5041 cattgccgta tctttgcctg tggaggatga atgatgattc gattgctcat tgcagatgac
     5101 caccccgtcg tgcgcagcgg attgagagcc atcctcgctt cacaacctga ctttgaactg
     5161 ctcgccgaag cagacaatgg attctccgct gttgaatctg ccgcaaaatt ccagcccgat
     5221 gtgattctga tggacctgca aatgcccgcg atggacggtg tgtctgcgat cgcgaaaatc
     5281 catgctgcga atccgtccat ttatattttg gtgctcacca cctacgacac cgatgtggat
     5341 attttgcgcg cgctggatgc aggcgcaaca ggctatcttt tgaaagatgc gccgcctgat
     5401 gaattgtttc gggcgatccg ctctgcggca cgcggcgaag tgaccctagc cccaagcatc
     5461 gccgcaaaat taacccgccg cctgacccac ccgctcgaaa gatctttaag cggacgtgag
     5521 atcgatgtgc tttccctcgc cgcacgcgga gactcaaaca aagagatcgc catcaaactg
     5581 catatcaccg aagcaacggt caaatctcat ttcgtgcata tcttcaataa actcaacgtc
     5641 accgacagaa ccgccgccgt gacaatggcg ttggagaaaa agatgattcg gctggaataa
     5701 aatattttca atgtgtgttg atgtgttttt ccagactcgt tatcaaacgc ttcatatcca
     5761 tgtgaaaatc ggggtcaggc cgaatctgga tcccgttttt ttcgcttaat ttttcgagac
     5821 ttggcggcag tttttctttt ggcggcatgg ttgccccacc taccagcaat ggaatgatgg
     5881 ggatttcatt gaccaacgct gattcgattt cgattcgcac aaaatcattg ggttcgtcga
     5941 gccgtgtttt tcctgcatcg tttgtctcca gccatttttc cccgatcacg gcaagtagtg
     6001 ccccagatct gccgattgcc tcgataatat attttccaaa atcagatccc agcggaattg
     6061 aatacacgtc tttaaaaacg tgctgcgccc caaaatgttt tacaagttgg tcataaattc
     6121 gccctacgac atcggcgctg tcttttcttc gataggagat gaaaataccg cccgatgcag
     6181 attcatctga tggcggggat tctgattccc cgctccgttg gtcaatgttg acggtctcgg
     6241 cgttgaccac ctgattgatg gattttgcat ttatattgac tttcatggct tccttttagg
     6301 agatgctgtt ctcctgaggc ttggctggga ggcgggcgtc ggctgtttgt aaaatcttta
     6361 tttcctgccc gatgatcagg cggtcgattt tttcggcgtt gatcgagtat tttccttcgc
     6421 ggtcacgata gtcggtaatg tcaaatccca tcgaatcgaa tgtgtcgagg atggttctgc
     6481 tgatctgttg gtagagcgtt ttgatcgaag cgagcgcctc tgtgctgccg aagaaatcgt
     6541 tcgtgaaggt cagagaataa ctttcccgcc aattttcgag cggagttaat cttcctggcc
     6601 atgttgtggt tacctccatc agctccttaa gttcctttga gaaatttttc tgttcatcct
     6661 ctgtttcgat cggattgagc tttttgatct ccgcataatg cattttgttt gatcggaatt
     6721 ttttgcttga ttgcttgatg ctttgtttga gcaggtttgt tttttgcgct gaattttgaa
     6781 actccaacaa cggtgtgggg agtgccgtgg cgctgatggg tgcatcttcc tgctccctgc
     6841 gatgctggag cagcctttcc tgaaaatagt cgtttctgtg tatcagcggc ccgattgtcg
     6901 ttaatgcgat ctgacaggat attgaattac ccgccataaa tggccgtaca aagaagtttg
     6961 ccactgtatc gtgttcaggg aaaaacgatt gaatgacaaa ataacagcgt gccgaagctc
     7021 tgctgtcgat tgagttcagg tttttcaggg tgtgcatgtc tgcccagagg tgtggggact
     7081 tgtccttgtt taaccatgcg ctatccatga acaggctgtc tccatgaatg gcgatcagat
     7141 tgccgcaatg cacttctttt agcgggaatt ctgcgatctt tccatgaatg ttttcctgta
     7201 ctttttcgat gatctcatgg atcgatccgc tgggtgtggt ttgttccttt ggtttgcaga
     7261 cgaacacatt ctgcgcctga agccacccat atcctggaaa aggatgcagg ctgtttgtaa
     7321 tgactatgat tggaattttc ttcccaccta tgattccatc cccaatctct tttaatttga
     7381 actcattctc gatcttgcgg tcaatattcg ggtctggttt tgcagtccag tttcctgcaa
     7441 ccaactgggc aattgctctt gcgcggatgt ctgatacagt tttcgggagt atctttaagc
     7501 tcccatacga tttatatatg aaaaatacca ggccgcctgt gaggagggct aagatcaaga
     7561 tttcaggttg ggatgtcgga ccaaggagga gaaaggcaga gacggcagtg agtgctccga
     7621 aacccaggct tttctgcaaa atacttttgt aaactttata tgatgatgac tttatttccc
     7681 cgatcacctg ccagatgatc tttaccaaaa gcttgtagtc aaataataca taatggccca
     7741 ggctattgaa gggtggcgca aacggttttg ggagcgattc gattaacggg aagtcacatt
     7801 tagagatgat ggaagctgcg atgtgataag tcagatgctt tgtgacattc tggcgtaatt
     7861 ggttgggaca atcaggcgcc gtatggtccg caatcagcgt ttcgggtatc gttgcgctca
     7921 ttttgcactt ccatttcttt attttcacta tacaggaaaa aaattggaaa agcaagttag
     7981 gcccgtggac aggcggatga ttgtcccttc ttcagggagt ttatattgat tatttaacca
     8041 ggtgaccgcc gctttcggct tgatcgagca cagccaaaat ggtgcggacc aattcacgtg
     8101 cggcggattc gctcaattcc acggcgacac gggctgaggg gccctgggct tcgttgacga
     8161 agtcaatgtt cagggcgtgt tcgtaggggg cgtcgaaggg atggtcgtag gagacattgg
     8221 cctgttgcag gggaaaccag ccttgggtgc cttttccgct gccttcaatt tttacttttt
     8281 caacgatcat tgtacacatg gtttgattcc tttttattct taaggtagat ctcttgcaaa
     8341 agtctgaaag attaaccacg gagacacaga gaaaataatt aaaaactccg tccgcaggag
     8401 gcgccgcact gcttcagtgt tttcgtggtt caaaatggct tatcaaaaca tcttttatgc
     8461 cagatgtttt tccaggaagg cgaaagtctt tttccaaccg tctacggctt gttcctggcg
     8521 atagttggga cggtcgtaat aaaagaagcc atgacctgca tttgggtaca tgtggaattc
     8581 ataacccttg ccgtactttt tcaactcggc ttcgtgaatc tttacctgtt cgggggtggg
     8641 gctagaatca tcttcaccga acaaaccaag aatcgggcag gataggtctt tggtgtaatc
     8701 gagcggggaa acggggaagt ttgcgttgag ctgatcgggc gtcatcacca cgcgcccgcc
     8761 ccagcaatct acgatggcat caaagcctgt tgtgcggcag gcggcgagat aggcgtgacg
     8821 tccgccggag caggtgccaa agatgccaac ctttccgttg acttctgcca gcccgcggag
     8881 gtgattcatt gcgcctgtga gatcgccgat gacctgttca tcgggaacgc ctccatctgc
     8941 gcggactttt gccgcgacgt cttcgggagt tccgtgcccc gagcggaaat acaaattcgg
     9001 cgagagggtc acatagccgt gatgggcaaa tttgaaggtg gcttcgcggt accactggtc
     9061 ccagcctggc atgtggtgga ccaataccat ggcagggaag ggacctttgc caagcggttt
     9121 tgccagatag gcattgatct tgtctccgtt tgcacctgtc atggtcacgg tttcagccag
     9181 cacgccttcg tacatgtcgg ttgtgtacat gggtcactct cctgagtaaa ttggtaaatt
     9241 ggcgcgattg tactacgctg tgcctttaac tgtcggcgct gggaatgttg gttttgggct
     9301 ggcaggtttc agttaagagg gaggcggggt tgtatccgac ttgaaatggg tcacaaatcg
     9361 agctacagtg aagttgcaaa aggtgtcctt tggaaaggag tcgagttatg gaaatcgcag
     9421 ttggaagagt aactcattac ttcgatcacc tcaaagttgc ggttttggca ctcaatggcg
     9481 acctgaagct tggagataag atccatattg ttgggcatgg cacagatctg ttccagcggg
     9541 tttcatccat gcagattgag catcgttcag tggtttgggt aaaacctgga gatcatgtgg
     9601 cacttaaggt cattgaacct gtccatgaac atgatgccgt tttcaggatc atcgaagaag
     9661 ttttggagcc gcttgtttga atttgaaaca aaaaaacaga gcggatttcc gtgaaatggt
     9721 tccgctctgt tttcgcatgc ttgttgggct gtgaatttaa aatgcaaata ctgttttgca
     9781 aatgatcggg ctttgtgtaa attatgatgc taccacgatg attggatcat gtcatgttcg
     9841 atctgtggag aggcaaggga gttgttccag atcttcatgt attgatcggc caactcgggt
     9901 ctgtagaatg aatgcagaat ctctccgatg tcatagattc catctgcaac gacagggtcc
     9961 atgagaaaga tgtaggtgta ggtgccatcc ttgtttggca ggctgggatg taaaacacga
    10021 actttgttgt atgtttcggg gtggatgtgg gcaatggcag gttttaggat ctcatgcaga
    10081 aaatgttcgt actccccgca tttatcggct ttgacatggt ttagcaacac ccaaacatcc
    10141 tctccctgat gggcacggat gtcttcgcgt tcccttgggt cgacataggt gaaagtcatg
    10201 gctgcctcct ttttatacat aacgcaaaat tgcattcgcg atcatttccg aaattcgagg
    10261 ctgtgttttt gtttttcctg cttttgtgca ggcggcaact tgggttaaag atactatagc
    10321 acgccaggga ggtggattca atctttcttt tgcttctccc gtctcagctt atgcaaaagg
    10381 tccatttaac ttgtggggaa aggcagaaca taaaagagca taacaaagaa aaaggttgtg
    10441 ttacctgcca gtacgcacaa gtgccagatc tcatggttgc caaaataggg cggaaatgga
    10501 ttcggctttc cgtaggcgaa gatcagcgcg ccaaccgtgt agaaaagccc gcccgtgatg
    10561 agcagggaaa tggctcccat gccgagttgg gtgtagatgg ggccgatgag gaagacgatg
    10621 atccaaccca tgagcaggaa gctgatgttc gacattaata cggagatctt tgtgaagaag
    10681 atggatttga gcgtgatgaa gaaaaccgca aggctccaga tgaccccaaa aagcacccag
    10741 cctgtggctc cgctgaaaat ggtgaggcag aatggggtgt atgtgcctgc gatcaggatg
    10801 taaatggcgt tatggtcgag aatgccgaag acccgcaggt attttccgaa aagcagaaaa
    10861 aaatggagga ggcagctgaa aaggaatgag agaaacaggc tgacaccgta gatcgcaaac
    10921 cccaccacgt ggatgggttt gtccgcgatg gctgccagtg aaacgagcac ggtcagcgcg
    10981 gaaatggaca gaatggcgcc gatgagatgg ctgatggaat tgaaatactc gttgctgtct
    11041 ggtgaggaga tgagcggatc aagattcgtt ttttgtgtgg tcatggtgcg ggagtatatc
    11101 ccgtaatggt aatttcctgc gggttgactt cgatctcaag cgcatcacca tattgaatgc
    11161 ctgcgatctc gtaggtgatg acctcgacca gcgatgggtt ttgaaaatgg gtctttttgg
    11221 tttcagggtg cggataatac acccagcccg catattcttt tccctcaaac ctgaccctgc
    11281 agcgcgagaa tgaaaacgtc tcgggcgggt gcaggtcggt ccactcaaca aggggaaagg
    11341 tgaactcggg ccgtgtcatt tcaaattcca gcggcgcgat ggaaatattc aatgtgccgt
    11401 tgaagtaggg agcaaggtca aggccgagcg ctttgaagta cggcttttgc ttttccaacg
    11461 aactgtacgg ataatctttg gacgggcggg aagccacttg atgtccacgc atgaggatgc
    11521 cgggaagata ggtcatggcc gcgagtatag cgcgttctgt gtgtaaagtg attaccatgt
    11581 tgtcaaattt atatgaattt tctttgccag ttttgaatct cttcttgtca actcaatacc
    11641 gcccgtgtat aatcctagcc atgaaacaaa ccctcaagca agccgcagca actcgagccg
    11701 ccgccgctac atgcgcgacc taggaacctg ccacggcttg agatggatga agaaatccag
    11761 tcccgaaacc aacaggttct cgggactttt tatttacacc gtcaaccttc aaccttggac
    11821 attcctcatt tataattcgc cagccatttc aaactttcgg agccactcat gagcaaaaaa
    11881 ctaacaggca accagattcg tcaggatttt attgacttct tcgtcgagca cggacatacc
    11941 gccgtaccct cgatgtcgct cgtcccaggc ggggatgcca cgcttctctt cacaaactcg
    12001 ggcatggtgc agttcaaaga tgtcttcatc ggcaccgaca caaaacccta cacccgcgcc
    12061 gttgattcgc agaaatgtat gcgcgtggca ggcaagcata acgaccttga agatgtaggc
    12121 cgtgatgaca cgcaccacac cttttttgaa atgctcggca actggtcctt tggcgactac
    12181 tacaaaaagg atgccattgc ctggtcgtgg cagctgctca ccgaagtgtg gggcctgccg
    12241 aaagataaac tctacgccac ctgtttcaga gacgacaagg gcaacgttcc gcaggatgat
    12301 gaagccgccg atatttggaa gcagcagcct ggtttcgacc ctgaacacgt cctcttcttt
    12361 ggacgtaagg aaaacttctg gcagatggct gaaacgggtc cctgcggccc gtgctccgaa
    12421 atccacattg acctcggcga cgagcgcgac aatatgcgcg gcaccgacca cgtctgcggc
    12481 gtgaatggcg aatgcacccg ctatctcgaa ctgtggaaca acgttttcat tcaatacaac
    12541 ctctttgatg atggccgcct cgaacttctc cctgccaagc atgttgatac aggcatgggc
    12601 ttcgagcgca tcgtttctgt tttacagggt gtcgactcaa actacaagac tgaccttttc
    12661 acaggttcgc ttgaagtgct gcgcgcgctc acgggtcaca ccgaaaagga aatgctcgcg
    12721 aatttcacac cctatcgcgt tatttgtgac catgtccgct cggcttcctt cctcatcgcc
    12781 gatggcgtcg tgcctggtaa tgcaggccgc aattatgtag cccgcatgat cgtccgccgc
    12841 gccgcgcggt ttggcaccaa gatcgggctc accgaaccct tcctcgcgaa agttgctgaa
    12901 gccgtcatcg aacaatatgg cgacttctat cctgagttac gcaagacaca acccgctatt
    12961 ttggataatc tgacacggga agaaatccgc ttcgcacgga ccgttgaagc gggtactgcc
    13021 catttagaga atctgctctc cgaactccgc tcgaccaatc agaccgttct cgatggtcac
    13081 aaagccttcg acctgtacgc cacctacggc ctgcctttcg agatctcccg tgatatcgcc
    13141 cgcgaacaag gcctggacgt ggatgagaag ggcttcacta ctgcgaagga agaacatgcc
    13201 attgcatctg gcggcggcaa ggccatgggc aaactcggcg gtgaagatgc agaattcttt
    13261 gcaggcatcc tgaaagattt gcaggcaaaa ggaaaattaa gcaaagacgg cgtggaatac
    13321 gacccctaca acgggctgga tgtttcccgc tattccaacc ttgaaattct cgctcttgtg
    13381 gtcaatggac aatcggtgga ctctgccaac ctcgacgatc aagttgaagt cattttgccc
    13441 aagaccggct tttatatcga agcaggtggt caggttaatg atacgggctt catccgcggc
    13501 gaaggctggg agatcgaagt cactggcatc cgtcgcgcgt ctgcgggtgt gatcgtgcat
    13561 attggtgagg tgatctcggg tcaccccaaa gtgggcgaca aagcgaccgc agaagtggat
    13621 gcgaatcgcc gccacgatat catgcgcaac catacggcaa cccacttgct gcacaaggca
    13681 ttgcatgagg tgttgagtga tcaggccaag caggcaggtt cactcgtatc cccaacccat
    13741 ttgcgctttg acttcaacca ccccgaagcc atgacccccg atcaggtgga gcgtgtggaa
    13801 cgcatggtca atgaagccgt ggcaatggat atgcccgtag tgaaggtgac caagtctttg
    13861 gatgaagcca aaaaagaggg cgcaatggcg ctcttcggcg agaaatacgg cgaaacggtc
    13921 cgcaccattt ccattatgga aggcggcgag gataaatatt cctatgagtt gtgcggcggc
    13981 acccatttag agcgcacttc cgatgttggc gcatttatca tcgtcagtga aagttctgcg
    14041 gcggcagggg tgcgccgtat cgaagctgtc actggccgcg gcgcgtacga cctcatccac
    14101 aagcgcttca aaaccttgaa gcaggcggct ggctctttga aggccgctgt ggaagaagtg
    14161 cctgcgaaag tggacgcctt gcaggatgaa gtttctgaac tcaagaaaga actcgccaac
    14221 ctgcgcacac aatctgctct ttccgcgttc aacctgcagc tttcaaacgt tcaaaccgtc
    14281 aaagatgtaa gcgtcctcgc agcggaaatt gtcaatgctg atacagacac gttgcgttcc
    14341 ctggccgaca agttccgcga gaaatatccc aaagcgggag cggctgtcct cgtaacaggc
    14401 acaactgtca tcgcagtggt gaccgaagac ctcgtcaagc gcggactcaa agctggtgac
    14461 ctcatcacag gcattggcgg caaaggtggc gggcgtccca atctcgcaca aggcagtctg
    14521 cctgatggca acatcaatga atcgctcggt aaattagcga aggttgtgga agagaaagta
    14581 aaataaaaac aggtcttgtg cctgattgtt tgaaacaaaa aaggagtgga ttttatccac
    14641 tccttttttg tttaattgtt ttccgcgatc agcggggtga tgtatttttc aagcatcgtc
    14701 atggtcactt cgcctgtcca cgcatagcgg acggtgcctg tgcggtcgat cacgtaggag
    14761 ttggggagac tgccgttgtg aatggcgttg atcgaggcgc tttcggggtc cagccagacg
    14821 ggaaagggca gccccaggct ttttgcgaat tcagcgacgg tgtcttttgc atcgcctgcc
    14881 tccacggcaa tgacggtgaa cccttcagcg gcatgcgtat cgtgatattg tttcaagatg
    14941 ggcatctctg ccttgcaggg tgggcaccat gtagcccaat tgttgacaag gacaatacgg
    15001 ccttgataat cttccagggt ttgcttttca ccattgaggt tttccagcgc gagttgcggc
    15061 gcagggaaat tcacttcggc aggcacaatg gagatttcat cggcggttgg caggcttgct
    15121 tttccgctgc tgcccaacag ccacaaaaag aaggcgaccc caaacagaat caacccgatc
    15181 cccattgcag tggatgaatt gcggcgtttt tgtcttttac cggggtattt ttgcttgttc
    15241 gcgtttgaca tggttttcct gcgatcttta gtttccaagc gcagatcgga tctctgccag
    15301 caattgctgt tcgggatatg cgccgacgac aatgccttcc ccggagttga tcacggtttg
    15361 aggcacaccc tgcacacgga agcgctgggt cagttcaggg aattccatgg catccacagc
    15421 ttccgcatgg accatgcctg ggttttccat ggcgatctgg tgcgcgagca acacggctcg
    15481 cggacaatgc ggtcaggtag gggtgacaaa gacttgtaaa tgcaggggtt tggtcaggga
    15541 tttgagaaat tcccttgttg ccgcacccag cccggaatcc cgcttggaag cgaagaggat
    15601 atcgttgatc agggtgctga attcataccc ggagggaatg cctgagaatt gaattcccag
    15661 gtcaacgacc gtatctccgt ccctgcctgc aatgacgatg acgggggctt tatctaccct
    15721 gaattgggcg gccagttccg catcttcgtg cagatcatgt gtggagaggt gaacgcggtc
    15781 atcgatctcg ctgatctctt tgaccagctg cccggtttcg ccgcagtatt cgcagttatc
    15841 cttgcttcca aagaacagga tctgcacagg gttggtgatg gcggcaaatg cctgcttgat
    15901 ctgaccaaca acctgttcgt tcaaaagttt gtccatattt ggctccattg gttttgtgtt
    15961 tcgtgccttt atttggatgc ccaggttgcc caaaagttgc aggtgatttt tgttatactt
    16021 tcggtgcaac ttttgaggac gaatatcatc caatgagtat tacgaccaca cctgaaattt
    16081 ccattgaagc cctgcgttca ggcagccgcg cagaatttgc gcgcatggtt gacgtgcatt
    16141 cccccctcat ttacaggctg gggctgaaaa tgcttgggaa cccccaggac gcggaagatg
    16201 ttttgcagaa cacatttttg aatgcgctga cacatctccc tgattttgaa ggtcgttcaa
    16261 gcgtttcgac atggctgtat cgcattgctt caaatgaagc cctgatgatg ctccgtaaga
    16321 aacggccgct caacattgaa gacctgcagg cagaaaactc tgatgaagga atggtgccag
    16381 agatttttgt ggattggagc gccctgcccg aaggcgagct gctcgcgggt gaaagcaaaa
    16441 acgtgatcga tgacgcggtt gcaaaattac ccgagcaatt aagaattgtc tttgtcttgc
    16501 gcgacatcga agggatttcg atcagagaaa ctgcagagat cctctcgctg acagagacca
    16561 atgtaaaaac aagattattg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 ngaaactgca gagatcctct cgctgacaga gaccaatgta aaaacaagat tattgcgcgc
    16741 ccgcatggcc ctccgtgaaa aattgtcggc gtactatggc gaacaacggc aaacacatgc
    16801 ctcgaaagga aaaaatgaac catcctctcc acgataactg caaggccctg ctgggctcgc
    16861 tctccgaata cattgacggt gaattgccgt ctgatctgtg cagggagatc gaaaagcatc
    16921 tcgaaggctg cgataactgc cgtgtcgttt taaataccac caagcggacg atcgaccttg
    16981 tgcagattcc tgtaaaagag gaatctgtcc ccgacgatgt gcgcgaacgc cttttcaaac
    17041 gcctcaaact ggacgactac ctgaatccac agccctaggc attttcttgc cttgtcaggc
    17101 tctcaaaatg accccgccaa agccattcat gtgaatggct ttttctttta aatccgctct
    17161 ggctgacagg gcaaagatac actccgaaaa ttctgtgcgg accacttttt cgctgccatc
    17221 aaaattcagc aggatggtca ggcttgtatg gtcccaggtt cgtctgtacc ccagaattcc
    17281 atggggaagg tttttgagta ctaccagtga gccttcctgt aaaactgttt cattcttccg
    17341 aattttcatc aactttcgga tgatgttcaa taaagaagac tcatcttgat ttgccttttc
    17401 cacgtttatt tcaaggtggt ttttgtgtac tggcagccag gtctgttctg aagaagaaaa
    17461 ccccgcattc cctgtcccat cccactgcat cggggtgcgg acttcatcac ggttgatggt
    17521 caggttgagc caattgaaga tgaagcgcgg aatgagtttg aatttatgcg gaattgggtc
    17581 gcgggcagca ccgaagggtt gcggcaggtc ggtcatgccg atctcttcgc cgtagtacat
    17641 gcacggcacg ccgcgcgtgg tgagttgaag catggcgagc agttttgcct tacgcaggtc
    17701 gttgcccagc ctgagaatac tgcgccgcct gtcgtgattg ctgaagacat acacaggcat
    17761 gaacggagcg gggaaatgtg cttcgatgcg ctcgagcaat tccctgaaat actctgctgt
    17821 aaattggaag ctaagcattt caaaatcgaa gaccagcccc agtccattgt tggtttcttc
    17881 gccgagaaat ttgcggagcg tggatttgtt tccgctgacc tccccgagta acatccgctc
    17941 gccgaattcc tcgcacacac tgcggagttc cctggcgaat tcgaaacttt cggcttgatt
    18001 caggttgtat tttgcttcct gaaaaaatcc agatggatca tcttccgtgg gcatgagttt
    18061 gaatgtgaaa gggttgtcgc gaaatgcggc gtccttgtaa atcacattga aaatatccag
    18121 ccgaaagcca tccacgcctt tgcccagcca aaagcggaca atttccagca tggctcgttt
    18181 gacttccggg ttacggtagt tcagatcggg ttggaaaggt aaaaaactgg accagaaata
    18241 ttgattgcgt tcaggggcat aatgccagcc gcttccgccc gtcatgcttt gccagttgtt
    18301 gggcttgtcc tgccagatgt accaattggc cttggggtta tcctgtgaag agcgcgattc
    18361 cctgaaccat gggtgctcgt ctgaagtatg gttcatgacc atgtcgaaga cgattttcat
    18421 gccgcgctgg tgaacacctt cgatgagttg cagggcatcc tccatgctgc cgtactcggg
    18481 agcaatgtcg gtgtagctgc tgatatcgta gccggtatcc gcttgcggtg aggaaaagaa
    18541 cggcgagatc caaagggtct cgaatcccag ttcgtgcaga taatccagct tttggatgat
    18601 gccctttagg tcgccgatgc catcgccgtt cgtgtcgtgg aatgaacgcg ggtagatttg
    18661 atagatgctt gttttgtgat accagggttt gtccatgttg ttattgaaag atgtctaaaa
    18721 agcggaacca gagtttgccc gcatcgacca cccaggaatt ttcttgaaat ggtgaagcgt
    18781 agggaatggg cagatcggca aatggattgt tgctgggttt tccgagaatg ccatttgcca
    18841 tttgccgccc aagatatgtg ccaagtgtga cgccatgccc aacgtagcac atcgaatacc
    18901 acatgccgtc aatttgacct gcatgggcgt tttcgtcgaa cgtaattccc attgtcccat
    18961 tccagaaata atcaattttg tggttcgcaa gttcgggaaa gaccttgttc attccgcgct
    19021 gcaaggtgtg gatattcgtt cgctggctgg tgttggcaaa agtaagttcg ccgccccata
    19081 ccatgcggtt atcaggcgag agacgatagt aagcaagcgt ttttcgtgtg tcgtatacga
    19141 cgcggttgtt tgggatgagt ttttttgcaa ggtcttcaga tagaggctcg gtggcaatga
    19201 tgtaactctc tacaggaagc acgcgccgac ggaattgcgg caggatgtcc ccgatccatg
    19261 cgttggcggc aagcatgatc tcttttgtga ggatgtcgcc tctttcagtt ttgacaagga
    19321 atcggctgcc gtcgctggca ggcgagtcgc ttatccgttc gatcacgatt acgcgcgtac
    19381 cttcgtgaat atccgctcct gcacgttctg ccgccattgc catgccacgc acgaatttgg
    19441 caggctgcac cgatccgctg catggattga tcatcatacc gttgtatgaa tctgtgccta
    19501 gttcggttcg tgtttcattt ctgggaagga cctgaacctc ataaccagcc acctcgtgca
    19561 gggtttcctg ctcacgcttc aatttttcga aatgggctgg cttgaaagcg gcttcaatat
    19621 tcccgcagcg gacgaagtca caatccagcc cttcttcggt gacgatctgt tccactgtgt
    19681 tggcggcata tgtggcggcg aggaacatat ttttcgcgag ttcccgtcca tgctctttta
    19741 tggcgttggt caaggtatgg tgcaggcagg aaagcgcctg tccgccgttg cgtgagctgg
    19801 cttcaaagcc gatcgttttt gcttcgagca aggtcacacg cgctccgcct ttcgccagtt
    19861 gcagtgcagc cgatgtgcct gtaaagcctc cgccgaccac gaccacatcc gttttggcgg
    19921 gcaatggttt atttttgtag gacgctggcg ttggggcggt ctcccaccat aagcaggtat
    19981 tttgaatcat gctggtttcc tcgttgcagg gttgatgtga acagattgta tatcaccact
    20041 tatcaaaact acaagttgat ttttgtgtct gggtgtgcca caatgacttt gacgttttga
    20101 atgggggaac tttccccgca ggcaatacgt ccaacccata tcacaaacct ggcgaataag
    20161 tgggacagcc tatattagaa aggagggcct atgacctgat aaaaactttt gaagctcagc
    20221 gggacgccgc ctgctgacct gatttactgt ttgtttctga aatataaaaa ataacaggag
    20281 aagaaaaatg aaacccttac gcaaagccat cggctttagt gttctattat tgctgttgtt
    20341 tacctccgct tgtggaggct ctgcgcccgc cgcgaccgaa gcacccgcag caacagaagc
    20401 cccggcgtca ccagattatt cttttgatgc ccctgctgca accgaagcgc ccgccgcaga
    20461 agcgcctgcc gccatcccgc agcaacagaa cgggcaacaa ccccctgcaa ctggcaacga
    20521 tccatatgac atgttcttcg aagactatgg cgtcaaccca tccatagata ccgaagacga
    20581 taacctttcc acatttgctt tggatgtgga tacgggctcg tacactgtca tgcgtaatta
    20641 tttgcaagat ggcaaccttc caccccagga ttctgtgcgt gtggaggaat acgtcaatta
    20701 tttcgatcac ggatatccca ccccgccgcc gcatcaggct tttggcattt atgtggatgg
    20761 cggcccttcc cccttcactg aaaccgaacg ctacgatatg ctgcgcattg gcattcaggg
    20821 ttacgaagta gccgatgaag accgcaaaga cgcggcattg actttcgtga ttgatgtttc
    20881 aggttcgatg gatatggata atcgcctcgg tctggtcaaa cattcgctgg aactgctcgt
    20941 tgagcaattg catcgaaatg acaccgtcag catcgtggtc tatggatcag aagcccgcgt
    21001 cgttcttgag ccgacccgtg gttctgattc tggaagaatt ctcgatgcga tttacagcct
    21061 gagccctgaa ggctccacca atgcggaggc gggtctgcgc cttggctatc aaatggcgat
    21121 ggaggctttc atgcgggatg ggatcaatcg ggtgatcctg tgctccgatg gcgtggccaa
    21181 tgtcgggttg acaggccccg atgccatcct tggggagatc aatcactacg tggaggaggg
    21241 cgtcaccctc accaccatcg gctttggcat ggacaattac aacgataccc tcatggagca
    21301 gcttgccgat aacggtgatg gcttctacgc ctatgtggat gacattgatg aagcccgtaa
    21361 gttgttcatt gaccagatca ctggcaccct gcaaaccatc gcgttggacg caaaagtgca
    21421 ggtggatttc aatccgcagg tggtgatgcg ttaccgcctg gttgggtacg aaaaccgcgc
    21481 tgtagccgat gacgaattcc gtgataatac ggtggacgca ggggaaattg gcgctgggca
    21541 ttcggtgacg gctttgtatg aggtcaagtt gtatcctgag gcacgcggca ggatcgccac
    21601 tgtgttcatg cgttgggaag accccgatac acatcaggtc gtcgaaattt caaaggattt
    21661 cgagaccagt cagatggcgt acgatttccg cgagacagac ccgtacttcc agcgtgctgt
    21721 ggttgtggcg gagttcgctg aaatcttgaa ggacagctat tgggctgagg aaagctcgct
    21781 tgatgatgtg tacgatgaag cccgccgcat cgccgattat ctctaccgcg atgatgtgat
    21841 ggatgaattt gtggatctgg tccgtgaggc aaggcggttg atggattaag aggctcacgc
    21901 ctttcaacgc cgataaaaaa taagtggtgg ttgaaacaac cgccacttat ttttcttgtt
    21961 atttgccttg cttgcctact taaacttcca attttgaatt ccccaactca tgctgcccca
    22021 agtgttgagc gtgtatccgc tcaggctgtc tgcgatgccg tagttatcct ggaacaggta
    22081 caggtacact tgcggcaatt cgtcgagtac ggcctgccct actttgcagt aggcatcttt
    22141 gcgggtttga atatcgaaac tgctgcctgc ggtatccagt gcggaatcca cttcagcgtt
    22201 gatccagcgg aagtagttgc cgcctgtggg attttcggcg gtgggaatgc gtgtggagtg
    22261 ataggcatcg aagttgtatc cctgcggctc agtggtgaag ccgcggtcaa agatcagcat
    22321 gtcgtaatca cccacggcgc gcggagagtt atcttcaaag gtgccgaaga tgatcgagaa
    22381 gtcgtagttc tgaatccgtg cttcgatacc cacggctttc aagttttcaa cgatgaattc
    22441 ctcggtgagt tgcagcggat cgaaggctgt gtacccttgc agttcgagcg agaggcgggt
    22501 tccatcctct gcgtatttcg caccctttgc cacacggata ccgtcatcac ccatgaccca
    22561 gcctgcttca tccaataatt tattggccgc ttccacgtca tagccaaatt ggcggggcaa
    22621 ttcgcacttg taccagccat atgcgaacgg attggtcgaa tcgctgacgc tgcctttcaa
    22681 aacatcctgt tggagtgact ggtaattgat ggcgtgggcg atcgcctgtc gtacgcgaat
    22741 atcacccaaa atcgggtgcg gcgcagaggc ggttgggtcg ccatcgaatg gcgcgctcag
    22801 gttgaaatcg atcgccatgt tccaaatacc ggggatgagg tattgctggg ccttccctgt
    22861 cagcagggtg tcgtaatcgg ctttgaattc gctgggccac aattgcattt gagcctcgcc
    22921 gttcagcatc atcgcggttt gggcggcagg ttcgggcacg acctcaaaca caacttcatc
    22981 aagatagggc ttgcctgctt cgtaataatt tgggttacgg gtaaagctca gccgcttgcc
    23041 cgattcccaa ttgctcaaaa tgaacggacc agcgaccaca ggattgctat tccacgccca
    23101 ggccgacatt tcttcgggtt tgcctgttgc atggcgcgga aataatccgt aggcaaattg
    23161 atcgagatag cccgggtagg gttctgaata ggttagaacg gtggtggtgg cgtccggtgt
    23221 ttctactttg gtgatcaggt caaaaccact cgtgccgacc aatgcacctg aatcagggtt
    23281 ggataatacc tccaccgtga atttgacgtc atcggaggtc aatgcttcgc catccgacca
    23341 tttcaaacct tccttgagtt tccaggtcac ggtcaggtaa tcgcttgaaa ggccgccatt
    23401 ttcggtggtc ggaacttcct gcgcaagggt cggaacaaag ttccctgttt catcaatggt
    23461 aaccaaccct gtcattgaag ttgcatccgc cacctgtcgc acgatcgcag catctgccag
    23521 ataaaaattc agtgtggtcg gttcttcagg caggatgagg acaacggttc ctcctccgct
    23581 ggaggcgggt tcggttgcag cggcatttcc tccccccgaa catgcgctta gcacaagccc
    23641 aaaaacgacg agcagggtga acatttttag tttcattctc tcttctcctt ttttgatttg
    23701 tttctattcg ctatttttcc gaatgaatta catacggatc gaacgcatcg cgcagaccat
    23761 cgccgatgta gttgatcgcc atgaccgtga tgaagatcat aaagcctggg aaaaaaccca
    23821 gccacggagc gcggaagatc tgattctgcg ccgtggaaag gatgctgccc caagtggggg
    23881 tggggggctg taccccaaac cccaaatagc tcaagcccga ttctgtgatg atcgccgatg
    23941 ccatttgcag ggtgccgctg accagaatcg gccccatgca attcggcaaa atatgctgca
    24001 caatgatgcg gaaatcactt ccgcccaacg cccgtgatgc ggttacgaac tccttttcac
    24061 gcagcaccag aaagagaccg cgcacaaggc gggcgggcca catccatgaa agcgccgcaa
    24121 tgatgatgat caccacaaag atgctgttct gcaaaatgaa taactgctgc tcccgtaaaa
    24181 acgcgccaag gatgatcagc acaaagatgg tggggaaggt caaaaaggca tctgtcacac
    24241 gcatgagggt ggaatcaagc cagccgccaa aatatccact caacgcgccg acggtaatgc
    24301 ccagcaaaat ggataacaat gtggaaagca gccccactgc cagtgaaacc cttccgccgt
    24361 acatgatgcg cgagaggaca tcacgtccca gttcgtcggt gccgaaccag ttggtagcgt
    24421 ttggtgtggc gaaactattg gtcgggtttt gttccagcgg gttgtgtgaa ttcaaccctg
    24481 caaagaccgc aacgaggatg agaatcaaca gaatgccgat ggcaatgcca gccagcttgt
    24541 gtttgaaaaa acgacgcaga acgatgcgtc ccatgctttg ctcgcgtgga agggaaatgt
    24601 cactcatatt tcacctgcgg atcaagccag ccgtaagcca ggtcagcaag aatattgcag
    24661 acgatgatca gcacagctgt gatcatcacc acgcccaaca gcacggggta atcgcgtccc
    24721 ttggcggcat cccagaacag gcggcccata ccgggccatg agaagatcgt ctcgacaaaa
    24781 agcgctcctg cgaacaagct gggcagatcg agcgcgatca gggtcaccaa aggcaggatg
    24841 gcattttgca aagcatgttt gtaataaaca gcgttatcct tcaaaccctt ggctcgtgcc
    24901 gtgcggacat aatcttcgtg cagcacatcc agcatgcttg agcgtagaaa acgtgagtac
    24961 cacgcgatcc aacccaaact caatgcgatg acgggcaaaa ccaggtgcca gaccgtatcg
    25021 agtaattcac cttcctgtcc gcgcgtgttc attccgccga caggaaataa ggggtcgcct
    25081 gtgaatggat ttttcagagt cacataaaac accacgatca accccagccc cagccaatac
    25141 acggggatcg attgtccgat gaaggtgatg gtggtcatgg tgtaatcgaa gagcgagtat
    25201 ggctttcgcg ccgataatat tccaattgga atggccagca tcagggtggt cagaaacgat
    25261 acaccgacga gtaacagcgt atttggaatc cgttctccga tcatttccga aaccgggcgt
    25321 cggaatttga tcgattcgcc caggtttccc tgaaacagat caaccagcca gcgcccatat
    25381 tgaacgggca agggatcgtt caagcccaat ttttcttcca gcgcttcgat ctgttccttt
    25441 gaaatgttcg ggttgcggcg cgcagcagtc agcgggccgc ccggtgcgct gcgcaccaga
    25501 aagaacaaaa gcacactgat gatgaacagg atcggaatgg tttgcaggat tcgccgcaag
    25561 gcataggttt gcatgattaa gcctcgaaaa cgatttttcc gtccacgatg gtcattgcgg
    25621 gtttggcggt catgatctct tctttgggca aactgaacaa atcatgtgag aacaaaacca
    25681 gatcagcgag ataaccttcc ttgatctgcc ctttttcgtt ttctttgaac tcggcatagg
    25741 cggcatcacg ggtgtagccg atgatgagtt cttcgagtga aagagtttgg tcggggtcgc
    25801 tgggattcca tttttcacgg ttcaatccca cgtgcaggtt gatcatcggg tcgtagggag
    25861 ccacgggcca atcgctgccg aatgctatgt tgttgcctgc atttttcatg ttgcgccacg
    25921 cgaacgaaag cggccagcgt ttttcgccaa cgcgggttgg ccagacatca ccttcggaga
    25981 tgctgaacgg agcatggctg gtctgcatgg atggcagcac gccaagttgt gtgaagcgcg
    26041 gaatatcatc gggatggatg acctcgatat gttcaatgcg atgacggcta tcccgcttcc
    26101 cattcgacct ttgaactgct tcgtacccat ccagcactcg tctcaccgca ccatccccgc
    26161 agcaatggac aaagatctgc agacccattt tgtcgcacag ggctgccatg cgggtgaaat
    26221 gctccagtga gtaaatacct tccgctgctg cttcggtgtc atcggcgtat ggttccagat
    26281 tcaaagccgt gtacgattcc cagaccccat ccatgaagaa ttttgcagcc ccgccgcggg
    26341 caaattctcc ctgtacgttc gccatctcgg cggcttcttt caacatctct tcagtggttt
    26401 caggtttgac ccagtatggc acatacacac gcacggtcaa ttcattcgca tcttcaagcg
    26461 cggcatagac catcatttct tccatgtcgc cgttcatgtt gtggacgctg gtgacgcctg
    26521 tggcgttgat gcgctgaatg gtcaatttga gcaattcacg cttgcggact tcatctggtt
    26581 cgggaatcag atcggagacc atgttcatgg cggtttcgcg caattcgcct gtcgccatcc
    26641 cgttctcatc acggacgatc acgccattac cgggtgcatc cacaccgggt tgaaggattc
    26701 ccgccatttc caaggctttc gtgtttgccc atgaggtatg cccatcgtag gcattgatat
    26761 acactgggcg gtctggaaca atttcatcca gttgttggcg gttttgcaca atattgtatt
    26821 taatgccgcg tccatccacc caggcggcgg tcttgttttc agttgcgaag ttttgaagaa
    26881 cggtcttcag gtcgttcagg tctttcactt cgtaaagctg ggcgctgcct gcccaaatgg
    26941 atccccacaa taaatgaaaa tgcgagtcga taaaccccgg ggtaacggtg cgtccctgcc
    27001 catcgatgac ttttgtggat tcatctttat atggttttgc cgcttcgttc gtgccgacaa
    27061 aaacgatccg attgcctttt acagccagcg cttccgcata cggcttggct tcattgctgg
    27121 tgaagatttt tgcgttctcg ataaccagat ccgccttggt agacattgct tctctcctta
    27181 ttgcttcgat ataaatgctt cgaatataca ttaactaagg gggtgatgca agtggtttat
    27241 aatcacgcca ttatggctaa ggaaaaagtt gttgtcgcca tgtctggcgg cgtggattct
    27301 tcagtggcgg cggccctgct gaagcagcag ggatatgacg tgacagggat gatgctgcgt
    27361 ttatggtctg aacctggcaa ggaagattca aaccgctgtt gtacacccga ttcgatggcg
    27421 caggcacggc gggtggctgg aattcttgat attccctttt atgtggtgga tgcgaaggaa
    27481 gtcttccgcg agacggtggt gcaatatttt cttgatggct acgagcgcgg cgaaaccccc
    27541 aatccatgcc tgatgtgcaa tcgccagatc cgctggactt ttttgctcaa tcatgcgctg
    27601 gccctgggcg ctgaattcat ggcaacaggg cactacgtcc gacgcaggag cggggatgac
    27661 ggacggacgg aattactccg cgctgtggac catgccaagg atcaatccta tgttttgcat
    27721 gtcttgaaac aggagcagct tgcgcatgcg cttttcccag tgggtgagta tcccaagcct
    27781 gagattcgcc gcatcgctgc cgactttggc ctgcccactg cttcgagagc agactctcag
    27841 gatctgtgtt ttctggcagg tgaagactat cgtaactttt tacagcgaaa tgcggctggg
    27901 atgttgaaac caggcaatat tgaaacccca gacggcagga tcattggtga gcatactgga
    27961 ttggctaatt acaccatcgg tcagcgtaag ggattgggcg ttgcctctcc tgtgccgttg
    28021 tatgtgatga caaaacgtgc cgcgagcaac acactgatcg tcggtgaatc tcatgagttg
    28081 ggttccactg aattgaccgt acgtgatgtc aactggttga gcggtgaagc gccgcaggag
    28141 cccttccgcg cagaggtaaa aacgcgctac acggccaaag aggtcacggc gctggttttc
    28201 ccattggaag agaagcaagc cgcagttaag tttgatgcgc ctgttcgaga tatcacagca
    28261 ggtcaggcgg ccgtgtttta tcaaggtgag gtgatgcttg gaggcggaat catcacccag
    28321 taagtttttg cgcgtagcca gaataaagca aacaagaaac aagcaatttt tgcccttgat
    28381 cgtaaccttc aaccttcaac cttcaacctt caaccttca
//
LOCUS       DAOQLC010000052        28381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_532_length_28381_cov_25.275542, whole genome shotgun sequence.
ACCESSION   DAOQLC010000052 DAOQLC010000000
VERSION     DAOQLC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_532_length_28381_cov_25.275542"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PL000_10725"
     CDS             <1..915
                     /locus_tag="PL000_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HNC89419.1"
                     /translation="DSVGVDVIALGEHHRVEYSISTPETVLAGIATKTNRIRLASGVT
                     VLSSDDPVRVFQRFATLDALSNGRAEVILGRGSFTESFPLFGYDLRDYEVLFEEKIEL
                     FAELLKEKPVTWSGTKRASLENVDVFPKTESGHLSTWVGVGGSPESVIRTARYGLPLI
                     LAIIGGNPQRFAPYIDLYKRAAAEFGTTPQAVGIHSPGFIADTDEEAWDLYYPQARVV
                     RDRIGRERGWPPSNLQEYQRERETGSLYVGSPETVARKIARVVKALGVNRFDLIYTAG
                     TQSISSRLRAVELFGTKVIPMVREILAG"
     gene            964..1668
                     /locus_tag="PL000_10730"
     CDS             964..1668
                     /locus_tag="PL000_10730"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HNC89420.1"
                     /translation="MKDNIYTMNIHTIGILGAGKVGTVLARLALSAGYRVLIGGSGDP
                     AKIALTIEVLTPGAVAVTAQEAASQADVVILALPLGKYRNIPVEALQGKLVIDAMNYW
                     WEVDGIRDDLTDPRSSSSEIVQAFLRESRVVKAFNHMGYHDLEEGAVPAGTSGRRAIA
                     IAGDDSNDVNTVAKLVDALGFDPVIAGPLAEGMRLGPGTEAFGANVGADELRAMLERF
                     PESERGKVILQARMVL"
     gene            complement(1800..2366)
                     /locus_tag="PL000_10735"
     CDS             complement(1800..2366)
                     /locus_tag="PL000_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC89421.1"
                     /translation="MLPNPDEKLDPRVRRTRGLILSSFESLLAEKNFEGISVQDVTEK
                     AQINRATFYAHFPDKYALLDYSMSLMFMSEIEKRTLNVCTYSPDNLRNLILAVCEFLS
                     HLHTGCAQPHQQFESLVEGTLKKQIFDLLSYWLRKSKLPTEIPATVATWGIYGLASHY
                     SHLKKRPALEKFVDEAYPLVAVNLEQFA"
     gene            2501..3145
                     /locus_tag="PL000_10740"
     CDS             2501..3145
                     /locus_tag="PL000_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC89422.1"
                     /translation="MNITIIGSGNMGRGIGTRAVAGGHSVTFVDANPEVAEKAAADVK
                     AAAKSGAKVSTASLGDAELGDVVVLAVWYGTNIEIAKQLGSKLAGKVVVDIANPLNST
                     YDGLATAPDSSSAEDLAKALVAGAKVVKAFNTTYAGTLMAGEVAGQKLDVFIAGDDAD
                     AKAKVAQLVTDGGLRAVDTGPLSRARQIEGMQLLHIVLQGSLGTNWGSALKILG"
     gene            3244..4155
                     /locus_tag="PL000_10745"
     CDS             3244..4155
                     /locus_tag="PL000_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC89423.1"
                     /translation="MNAQTNAAKTEFSIHPATKLGLVSLTVANLENQILFYTQALGFK
                     VHWREGNRAGLGAGGADLLLMTEESNLKRYRGVTGLYHFAVLFPNRRELARAVARLSV
                     LKYRNHPTDHIMTKTTYLDDPEGNGIELYCESPEDGSFVIENDDFVTRRTDGSWSDGR
                     EPLDVEALFSHLKDDDRLDDPLPAETRVGHVHLHVRNVQEAVDFYHGIIGFDVMGNSS
                     KFQAAFISAGGYHHHLGLNAWKGEGAPPPPADAVGLRHFTVELPNQQALDEVVKRIDD
                     AGIPSNKTDQGLLLYDPSQNGVMLKVG"
     gene            complement(4442..6931)
                     /locus_tag="PL000_10750"
     CDS             complement(4442..6931)
                     /locus_tag="PL000_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC89424.1"
                     /translation="MTHHRAKNIFIAIFLVLFLFGLRGSLAERAQAAPAQAVTAFLYP
                     PYPGTASQESVFDHTSPNYVADNRVVTFNGAEGKKTCPNPPPAGTPPPEAGVCDIGYG
                     GYWSYKLGDWLFYDGHDGIDYGMSYRPLYAAADADQVIYSGWWDPQNHKVSLGLYVRL
                     HHPNGYSTSYGHMSAVAVSACSTPGCVFIPHGEWIGFSGSTGNSSGPHLHLRVVNPSG
                     KAVDPYGWTGSGTDPWPSNQKESLWVQYPSLIYYGAAIFPSGSALGYPPAPVNGIVVD
                     DGGAGFSESPAACWKTGTATNATNGAMRYVKPRTSTATCSAQWEFPQGSTAGMYSVYI
                     RVPSNHATVEGAFYDIQHAGKTARVLINQAVYPNRFYAADGWIYVGKYNFTGTGSEFI
                     RLTNQTQDESAAIADMELGADSVQFIYQGDATPTPPSIVTDTPTPTRTLTGTPTKTFT
                     PTITRTPTATRTATITRTPTITRTPTITRTPSKTPTATNTKPATATPLYTKVKLYFVN
                     RFRLKANTPPYEVFGTRYLSSSSNLPEGVLKEYFKGPGSTEYGWGYTAIYNGFTGFNK
                     LEIVGDVAHVHLKGACVSSGKDFNIADLITLNLKQFGTIQSVKIYDQFDQTQNPSGAG
                     DSEPICLDPSFTATATASITPTASRTPTPTKTPTVTRTPTITKTPTNTRTPSRTPTPT
                     NTKLPSATPAYTKVTVYFVNKSKYNANTPPFEVEGKRYASSSADFAKFVLDEYFKGPG
                     LTEKNDYGWTAVFSGFTGYSKFEVKEGVAHVYLKGTCNSAGRDYNLADILMVNLKQFS
                     SRIQFVKIYDENGTTQIPDGASDSIPACLAP"
     gene            complement(6952..7629)
                     /locus_tag="PL000_10755"
     CDS             complement(6952..7629)
                     /locus_tag="PL000_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89425.1"
                     /translation="MRKLITLLSIFFITACAAQNAAMSAPAQKVEAQQEFESPTAEPP
                     TMTATALLETETATATLVPPAMTPTETPLPPLELPTPLANPPALLAWDGTPTYLGDSK
                     PDYLFRVRYDPEIWALTTDSYGYPAIGHRGIEYCVIAPAFGRGLPSNTTVEHDIRKIG
                     VVEFEINTVFRGGVRQSVTYLGGDTNIYTGFEVTFAENADLCLQEAEMVFATFTSVKV
                     TQATPVP"
     gene            complement(7700..8785)
                     /locus_tag="PL000_10760"
     CDS             complement(7700..8785)
                     /locus_tag="PL000_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89426.1"
                     /translation="MCDDYNTLMKSHISALISFILLLSSCSPQPPAKNILAAIRLDPP
                     ALIQFSEDITPLGELPFSLPADCGIYDIFSPSRGSTLAVEISCSFGQALLFLDTETGS
                     VTQAFPQGDSHLLAWASNGKSVFLRVDNASDPRILRVNAETLAAEIIPINEFTYDLAP
                     KPESFDFTFTYSRGFGFGSEIQLAKDDGRIVQLLYADPLNYISFARWSPNGKQIAFIK
                     IPDSQTPFTVGELWVMDADGSNPRKLADADAGHGYAANWSPDGKRIAFIVRENAEDEA
                     ANVSADALISNLYVVDVTTDELTQITNFSEGRIETAHWSPDGNALFFLRVLNGKMELQ
                     TAPSDQLSAEIKALLAEPACCLSWMRK"
     gene            8828..9592
                     /gene="surE"
                     /locus_tag="PL000_10765"
     CDS             8828..9592
                     /gene="surE"
                     /locus_tag="PL000_10765"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC89427.1"
                     /translation="MHILVTNDDGVQAPGLLALAKEMRKLGKVTVFAPDKNWSASGHV
                     KTMERPLRVREVLLADGSPAFASDGAPSDCVALPLLGLIEKQVDLVVSGINPNANLGH
                     DVTYSGTVTAAMEAVIAGVKGIAVSLDSPEGFKGKLDYSTAAIVARRVAKKVMADGLP
                     EGVVMNVNVPYLKKEKLKGYMITRQGLRVYRDALDERVDPRGKPYYWIGGEAPTGVDE
                     PGTDFGALRAGYVSITPLQLDLTHYKAMDVLRKWKF"
     gene            9615..10235
                     /locus_tag="PL000_10770"
     CDS             9615..10235
                     /locus_tag="PL000_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HNC89428.1"
                     /translation="MTQYFFVDESGDAGLAGQIGSSSHFVVVMVQLPERTKVNPLVEL
                     RDSLNLSPHFEFKFHQSKPWQKERFFNELLNFPFRIRAAIVDKSQADFHPQEISPRQL
                     IIDLIVGLTMRADELDVSNDILIVDGATPTFCRDLRVSFTEQCRQKKRIRPFKNIVGA
                     NSKNEDGLQIADMVAGAIRLHSIGALDGYFHTLLPKIVDLWHFPEK"
     gene            10406..10687
                     /locus_tag="PL000_10775"
     CDS             10406..10687
                     /locus_tag="PL000_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89429.1"
                     /translation="MDFFKKLFSGTPAKAQKRYHTFSVKCLRCGEVVEGRIDLDNELS
                     VEYESNRDVYYGRKVLMGSGRCFQRMEVELKFDYNRQLLDQQVSGGEFV"
     gene            10687..11154
                     /locus_tag="PL000_10780"
     CDS             10687..11154
                     /locus_tag="PL000_10780"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNC89430.1"
                     /translation="MDESIVDHYWYSFLHSLPPDSRYFGKLYVAERFGDNSALAQELG
                     QLVVDGVKTGTCSAIWEWEAEGKSIPQPGLITIVLDGNDQPMCIIETIEVFACRFKDV
                     DEEFAEAEGEGDRSLEYWRSEHKKYFSRVLPKIGREFSEDMPLVCERFKVVYK"
     gene            11154..12674
                     /locus_tag="PL000_10785"
     CDS             11154..12674
                     /locus_tag="PL000_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89431.1"
                     /translation="MRRLSNILLILLAGLLVVQAGLSLRWPIAHDEAPLLYEAFLMHS
                     EGRVPYRDLFDFQMPGSFAAYYLLGRLSGFNGFFRLRLLDLGILAALLAITFLFMRRF
                     GRQTAFAAAILFGLKYMQGGAFMSLQREYLLLGFVALAVWVSMRDVLTYKHRLLIGIL
                     FGLSAVIKPHAALGLLPILLFDITSLIQRQKLTLIKSAMTSIPPVTIGFAMPILAMIG
                     WLAATNALTPFLDIVLHYVPLYAQINGQMEITEAGTRIPFLLNQSLRLGGHALWVIPA
                     ALGVYLVPQENKRQAWLLAGLAACYAVYPAFSGQFFEYHYIPFIYFIVILAALALTEQ
                     QPLSTFHSLPSFLFPLSSLILLFAIAFAVRPSNTFLRQIHNRPIAKTTDRAEEIASYL
                     EENMQAGDTVQPLDWTGGTLLAMLETRAPLATKYVFDFYFYHHISTPYIQNLRADFMD
                     ELQEADPRFIVEVIAVDKPWLTGPDTSREFPELRAFLKANYSVTILEDDYLIYKRK"
     gene            12735..14300
                     /locus_tag="PL000_10790"
     CDS             12735..14300
                     /locus_tag="PL000_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89432.1"
                     /translation="MKHLFRITYLYLTIPFIIFCMGWLRLSVAIPVVAVIVWALWQLL
                     KQTPPGQDSKITIHHLLLALLVTGIWLFLSGVGGYAFQNWDHHWRNAVFRDLVTNNWP
                     VIYSSPERGPITMLVYYLGDWLPAALFGRVFGWSIANFMLFLWTWLGVLLTVLHLNLK
                     LKTSLVRSALLLIFFSGLDVVGALLFAKEYPTLWPPITHLEIWSGGDLQYSSFTTQLF
                     WVFNQAVPAWLCIVLILGDNVIARRVLSPTTQSSIHKEIASGIEQDRPRYDIKFFIWA
                     LSFFFAPLASIGLMPYLMVDFFTQADWRSFFKSIRVDLLFAAGIIFLLSYFFFSSNTA
                     AQERGFHSLVLKDWLFFFLLDGGIFWLMLAPFKWRDPRWAVTGILLLVMPFIQLGNGR
                     DFVMRASIAPLFYLMMMVGETIFKNTTARGLRFAFYVLLFLGALTPLYEINRSAVRTY
                     EYYFVLDETQRAQPNVEPATHLEKGGAPELEHPNAILADDIYTLTFMQDKLSKNFIAN
                     VRQSLYYRYLSSR"
     gene            14328..14696
                     /locus_tag="PL000_10795"
     CDS             14328..14696
                     /locus_tag="PL000_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNC89433.1"
                     /translation="MTQQFPSLPNPQAFYAEVWNIVCLIPRGKVASYGQIAKMIRPPA
                     GVDPETYLEFGALWVSNAIAASPNEVPWQRVVNSKGEITERDGLEAKRHKLLLEDEGV
                     PFGIRGRIDMKKYGWSGKSK"
     gene            14748..15137
                     /locus_tag="PL000_10800"
     CDS             14748..15137
                     /locus_tag="PL000_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNC89434.1"
                     /translation="MPSFSSPPNPQAYYEQVWAITRLIPRGKVATYGQIAKMIPPPNG
                     VELEAYAAFAPRWVGGAMANCPDDVPWQRVINSQGKISERPGAERQRPLLEAEGVVFD
                     AKGKVDLKKFGWSGKDESDIPQQTAMF"
     gene            15236..>15506
                     /locus_tag="PL000_10805"
     CDS             15236..>15506
                     /locus_tag="PL000_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89435.1"
                     /translation="MSLLIIGGTSTDILHLESQTVPSAGGAGMYTAMAAHRCGSNVSL
                     FAPKPNPIPDVLQPIADRLTEWFGPIVDPEDGLPHFEISQRGGKTE"
     assembly_gap    15507..15606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15607..17178
                     /locus_tag="PL000_10810"
     CDS             <15607..17178
                     /locus_tag="PL000_10810"
                     /inference="COORDINATES: protein motif:HMM:NF021832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNC89436.1"
                     /translation="PEDGLPHFEISQRGGKTEYLKATFGAESTLTPDMLPNDLSKYDC
                     IHVVPLGDAQKQLDFITACRAHGAKKISAGTYIAGVDKSPHLVHDIIAQTDIFFMNQS
                     EAIGLFGSLEAAHTDSGKLLFITLGADGACIIQGDHKTFIPSVPTTELDPTGAGDTFC
                     GATLAYLAQGAHPIMAAQHAVPLAAQMIAHVGPAALLFNEPAPEIPASPKAQTNPTQI
                     QKISKMVAALDEAAPFNFISPELPPAHHPKALDYFFAVTLQQFSFWNEQNNRYHTPLI
                     APLGGVSQKGAFYLFDAYRRRLESDADFCSPERQANLSRAELLEIFKADDGTDPMPAL
                     DLHLEMANAYGRDMLALHLTPEAVLQKARASANPLATLISILDKIGGYKEDPLRKKSG
                     LLALILNQRPEAFLTFAAGEQVEPVIDYHLMRSCLRVGLVDILDEDLKTRITNRELVS
                     PAEEWAIRHTAYRAIEEVVALSGKSTGAVDWFFFGARKRCPEMTEPECGLCQIDSVCA
                     HRKELFQPVLRTTFY"
     gene            17238..17609
                     /locus_tag="PL000_10815"
     CDS             17238..17609
                     /locus_tag="PL000_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89437.1"
                     /translation="MQSPEKPTKFGTISFILAIVVVVLWCVYFILFAAMTEGGATFGM
                     DGETAGYALVLGGGTVMAVLTVLITLAGIILGILALRKQDPKRGLAITGLVLNFLCFA
                     PYCLLLLLIAFGSISTADFIP"
     gene            17872..19176
                     /locus_tag="PL000_10820"
     CDS             17872..19176
                     /locus_tag="PL000_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89438.1"
                     /translation="MRKWRGTFYVLFLAVLMMLLSSCAPKVTLTYRVSGTATKALVEY
                     RGVDGEKTQESVTLPWEKSMEVGEDFRYELYVMNENEGGDIQCEISADGISFGTAEGS
                     MYTGCSGVVRTDGKTTSSFMTTLNDTYPDLSDLPYKEQGRFLIYTSQKFDMGEIVIKD
                     LKTGEEMQMTDGLRGGYCLSKSPVNAKVAFTSLRDSVNGDIYILDINARKGEELTNIT
                     NTPDTYEMCPSWSNDGQMVVFTFKSSETSPWEIGKIGYYGGDVSQITNSTAGENSLYP
                     AWSPDGAKIAYVVNNEDGDTVYVMNADGSNPAPLSETFASAFSVSQVAWSVDGQQLAF
                     FQHNDDLVSLFILSADGNVEKRIEMDVARTGSLSWDAEGKQIVFHAWKNISESADIYS
                     INLESGEIMILAENPTLDFLGPFLNTDDTITSFPRRTIELAQ"
     gene            complement(19259..20227)
                     /gene="holA"
                     /locus_tag="PL000_10825"
     CDS             complement(19259..20227)
                     /gene="holA"
                     /locus_tag="PL000_10825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNC89439.1"
                     /translation="MPNIFLLYGNDEFAISRKLKDFESDFTDPTTADMNTTRLEARTM
                     TENELNNAVNAMPFLAPKRLVFISGPSGKFNNPSTRKKFLEFLEKAPDTAKIVLFETI
                     EPKEAEKHWLTKWAEKNKPQVKPLGFFLPKQRDMPGWIINEAKNLGGQIDPPAAARLA
                     EMVGVDTRQAGMELSKLLAYVNWARPVHGSDVEAVCIVTSQQSVFDFVDALSQGNGKS
                     AQKLLHRLLENEDPFSLWGMVVRQFRLLIQAREILDGRGNKDDVARALGVHPFVAEKT
                     TGQAARFTMESLESIYHRLLGIDERVKTSQITLDLALDTLVVELAR"
     gene            complement(20261..20707)
                     /locus_tag="PL000_10830"
     CDS             complement(20261..20707)
                     /locus_tag="PL000_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89440.1"
                     /translation="MSTETPTNFRVNIHWALQLFLLVLCIPVIWGLIYLLTKMFAAIS
                     VAGSIEIVFLLVFLWFLVSFAFSAIEVNHSSISLHAPFGTTSINWDDVEIITLKGELF
                     TIQTKGKKKHLSVSMEIAGKGKKEFYEFINKRIESERIIVQKQSKH"
     gene            complement(20723..21208)
                     /locus_tag="PL000_10835"
     CDS             complement(20723..21208)
                     /locus_tag="PL000_10835"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HNC89441.1"
                     /translation="MILDLPFILETRRNHALEHATLHMLAHTHTGTMAGHSNPTGFFL
                     FGNFSTQDVWAAATEALERLRGGESGLAIHAGCGTNMATTALLAGTFAWSVLRLAKST
                     LMKILLMPLAVFLGVVGFLISRPLGPVLQQRITTEANMGNMQIIDIIPIRKGVHRVIT
                     K"
     gene            complement(21256..22251)
                     /locus_tag="PL000_10840"
     CDS             complement(21256..22251)
                     /locus_tag="PL000_10840"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNC89442.1"
                     /translation="MNTKASPLQVPSFHIREVPIHGDTILAPMDGYSDWPFRSLCREL
                     GSAMSYTEFIKVEKILSRSKQPAKRMYFTEPERPITFQIYGDDPDLILRAALQIQENN
                     PDVIDLNMGCPAKTIADRGAGVGMMPTPIKIARTFRKLVKHLHVPVTGKIRLGWDRKK
                     NYKLIARIVEEEGGSLIAVHGRTKEQRYAGNADWDAIAEVKSLVKIPVIGSGDVKTVA
                     DIDRMKAHTNVDAVMIGRGAIPNPWIFSRLDRDQVPPEMLKQTIRKHLARCVEFYGDE
                     DGSRLFRKNAVQYVMMQHLTRDERKEILKTRPSAEFLELLEKIYDSPIIPQPAIP"
     gene            22429..23868
                     /locus_tag="PL000_10845"
     CDS             22429..23868
                     /locus_tag="PL000_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89443.1"
                     /translation="MNRKILVAIFLLIFTFAQVGNASAATVEGTVTLVELPTFSNIFD
                     VDLEWSVSGMSGGATQNIFVWGRQQEAPSVAYDPSDCRLAASLPGVSDAAGTYTYQYF
                     FSAFDQQTWEFTLTVDDAVDCNSATLPSTNSVMGSTFIDGWWLDGFIANPPSLDPAFF
                     EEIPVACNTFELWGLVTDRVPDRVAKTHGGEASTLGYSGFASWNQSITGTFTPPAPLG
                     PPDALLSWVYTFPSTASGLWSFSVDPTDYAGNNWGTTNFRYSFISEEELADCVTFSDV
                     SETDTEIYVRYMADLDLAHGYLDGTFLPDATLTRAEMAAFIELANGIQGDDPEFATPP
                     PSGSACDFSDVSSDDWFAGWVWQACADGYMNGIGNGLFDPNNYLTRGQVVTVLDNINK
                     MGTGSGYLATQNVFQGQWGNLYREAVFLDVAVGDFYAGPVQRAFGVGVVDGTSDTTFS
                     PDQAILRGEFIKWMYRTLSRIQPGCVECQ"
     gene            complement(23917..24468)
                     /gene="msrA"
                     /locus_tag="PL000_10850"
     CDS             complement(23917..24468)
                     /gene="msrA"
                     /locus_tag="PL000_10850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNC89444.1"
                     /translation="MNTNYETITLAGGCFWCLEAVFDEMKGVLSVDSGYSNGHVPNPS
                     YRDVCSGTTGHAEVVQIKFDPTVISFRDVLNVFFAIHDPTSLNRQGADVGTQYRSGIY
                     YHTPEQKAAAEQAISELNTQGIWSSPIVTEVEAVHNFHIAEDYHQEYFVRNQFQPYCQ
                     AVVAPKVAKFRKHYLELLKKQPA"
     gene            complement(24617..25666)
                     /locus_tag="PL000_10855"
     CDS             complement(24617..25666)
                     /locus_tag="PL000_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89445.1"
                     /translation="MNKQELLKLADYNFQRGNRELAKKYLVELLTLHPEDEPAWMLLA
                     RVVEERERQVECYRKVLKINPGNNEAKIALARLESKTQPLLVSTYQQQKAAAAKAPAR
                     NVLRGVMIAVAVLIGLGATTFAIASRNPQSNMAKLIIPASPTPYTQTLAGDVAPQTRA
                     DIVKKYPEYAPLVDTLISFAISNAESGMVGAPERPGAEIMPSDTAASQAKEAFESALP
                     QPGTLNTATLSEEQITSWLAVEMRNSPDLPLQDVQVYLRDGKIQIWGVVKGSADSTSA
                     LMVGSVSLDANGLPGFEIESMQIGQQVIPDVLVTQAESWLNQMLLDEINRQVPGLRIM
                     NINISSGMITVAGMR"
     gene            complement(25717..28203)
                     /locus_tag="PL000_10860"
     CDS             complement(25717..28203)
                     /locus_tag="PL000_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNC89446.1"
                     /translation="MAGMERFTQRARRVLSLAHQEAERARQNNIGTEHLLLGLMDEEG
                     GVAGRVLRDLGMTPERVREVVQRVSGSAVNFDPNRVELAPETQQVLEFAVDEARRLSH
                     HYIGTEHILLALVRVESTGLEALRRLGVTADQVRRQTRRVLNESASSSSPTPAGPQPG
                     KGGAANASPKTPLVDQLASDLTSKAEEKKLDPVIGRQKEIERVIQILARRTKNNPALI
                     GEPGVGKTAIVEGLAQRIVDGDVPAPLMNKRVLQLDVASLVAGTMYRGQFEERLKRII
                     DELKQSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTMDEYRKHI
                     ESDAALERRFQSVQVDEPSEEETIQILKGIRSAYEEHHHLVISDEALEAATHLSSRYV
                     TERFLPDKAIDLIDESSSRVRMYKSPAAKHAKDLFGQLRQARQNRTLAQEEGNSEDIK
                     EWEERELDLNQQIERLRTGWDRTTSPVVSAEDIAEVVSMWTGVPLMQLAEAESQRLLK
                     MEDELRKGIVGQEDAIIAISRAVRRARAGLKDPKRPIGSFMFLGPTGVGKTELTKSLA
                     KFMFGSEDAAIQLDMSEFMERHTAARLVGAPPGYVGYEDAGQLTEALRRRPYSIVVFD
                     EIEKAHPEVHNMLLQIMEEGHLSDAKGHKVDFRNAIIVMTSNIGADMIKNQTSLGFAM
                     KRDEATEERLSYEDMRKKLNESLKRAFRPEFINRMDSVVIFRSLNKEDIQKIVSLELS
                     KVSDRLVEHNLVLTASEEALSSLATMGYDSEFGARPLRRVIQQRVEDPLSDRLLAGEF
                     INGDTIFVDVNDDGEVVLNKAAEKQPEPSV"
BASE COUNT         6315 a         7044 c         7681 g         7241 t
ORIGIN      
        1 gactccgttg gcgtggatgt gatcgcgctg ggcgaacatc atcgcgtgga gtattcgatc
       61 tcgacgccag agacagtgct ggcgggcatt gccacaaaga ccaatcggat tcggctcgct
      121 tcgggcgtga ccgtgctgag ttccgatgac cctgtgcgcg tgttccagcg ctttgccacg
      181 ctggacgcgc tctcgaacgg gcgggctgaa gtcatccttg ggcgcggttc cttcactgag
      241 tcttttcccc tcttcggtta tgacctgcgc gactacgaag tgttatttga agaaaagatc
      301 gaactgtttg ccgaactgct caaagaaaaa cccgttacgt ggagcggcac caaacgtgca
      361 tcgcttgaaa acgtggatgt tttccccaag accgagtcgg ggcatttgtc cacttgggtg
      421 ggtgtcggtg gttcaccaga gtcggtcatt cgtacagcgc gttatgggct tccgcttatc
      481 cttgccatca tcggtggcaa tccgcaacgc tttgcccctt atattgatct ctataaacgc
      541 gccgcggcag agttcggcac aacgccgcaa gccgttggga ttcactcacc tggtttcatt
      601 gccgacacgg atgaagaggc gtgggatttg tattaccccc aagcgcgggt cgttcgtgac
      661 cgcatcggca gggagcgcgg ttggcccccg tccaatttgc aggaatatca gcgtgaacga
      721 gaaacgggtt cgctctacgt cggttcgccc gaaacggttg cgcgaaagat agcccgtgtt
      781 gtcaaagcgc ttggtgtgaa ccgcttcgac ctgatctata ccgcaggtac acagtccatc
      841 agttcacgtt tgcgtgcggt ggagttgttt ggcacgaagg tgattccgat ggtgcgcgag
      901 attcttgcag gataagcctg accgctgacc gcggtcgacc gttaacggtc tgccgtccaa
      961 gatatgaaag acaatatcta cactatgaat attcacacca tcggaatttt aggcgctggg
     1021 aaagtaggca ccgtgcttgc gcgacttgct ttgtcggcgg gatatcgcgt actgatcggc
     1081 ggttcaggcg accctgcaaa gattgcgctg acgatcgaag tcctcacccc tggcgcggtg
     1141 gcagtcactg cccaggaagc cgcgtcacaa gcggatgtgg tgatattggc acttccgctt
     1201 ggaaagtacc gcaacatccc tgtcgaagcc ttgcaaggca aacttgtcat tgatgcaatg
     1261 aattactggt gggaagtgga tggaattcgc gacgacctga ccgacccgcg tagttcatcc
     1321 agtgagatcg tgcaggcatt cctgcgtgag tcgcgtgttg tcaaggcatt caatcacatg
     1381 gggtatcatg atcttgaaga gggagccgtg cctgctggaa catctgggcg tagagccatt
     1441 gccatcgctg gtgatgactc caatgatgtc aatacagtag caaaacttgt tgatgctctc
     1501 ggcttcgacc ccgtcattgc tggtccgctt gcggaaggga tgcggcttgg acctggaacc
     1561 gaagcattcg gtgccaacgt cggcgcggac gaattgcggg cgatgcttga gcgcttccca
     1621 gaatcagaac gtgggaaggt gattctgcaa gcgaggatgg ttttgtaata aaaaatctta
     1681 tctgcagtag ttgatttgtt tataagcaga ccgccgaccg aagactatag tcttcggtcg
     1741 gcggtctgcc gtcagtaaat gttatttttt tcgtcgcttt tcttggatac ccttgcggtt
     1801 tacgcaaact gttccaaatt gaccgccacc agcggatacg cttcgtccac gaatttttcg
     1861 agggcggggc gttttttcaa gtggctgtag tgagaagcca atccgtaaat gccccaggtg
     1921 gcaaccgtgg cagggatctc ggtggggagt tttgatttac gcagccagta agagagcagg
     1981 tcaaaaatct gcttcttgag cgtgccttcc accagtgact caaattgctg gtgcggttgg
     2041 gcgcagcctg tatgcaaatg ggaaaggaat tcacatacgg caagaattaa attccgcagg
     2101 ttatcggggg agtatgtgca aacgttcaat gtgcgttttt cgatttcgct catgaacata
     2161 agactcatgg aataatccag caatgcatat ttatcgggga agtgtgcata aaatgtggcg
     2221 cgattgatct gcgctttttc ggtcacgtcc tgcacggaga tgccctcgaa atttttttcg
     2281 gcgagtaaag actcaaagga tgacagaatc aagccgcggg tacgtctgac gcgcggatcc
     2341 agtttttcat cggggttagg caacattttc actccagtgt tgggtatcca acaaatcaaa
     2401 atgattgttg gttgatttta tgggcaagac tggtaaacta agcaacatct gttgtttaca
     2461 cagcaaccgt atattatcac tcataacaag gagagtcaag atgaacataa caattatcgg
     2521 atcaggaaac atgggacgcg gtatcggaac ccgcgcagtc gctggtggtc actcggtcac
     2581 ttttgtggat gcgaaccctg aagtagcgga aaaggctgcc gctgatgtga aggctgccgc
     2641 taagagcggt gcaaaggttt ccaccgccag ccttggtgat gcagaacttg gtgatgtggt
     2701 tgtgctcgcc gtttggtacg gcaccaacat cgaaattgcc aaacagctcg gcagcaaact
     2761 cgctggcaaa gtcgtggtgg atattgccaa cccgctcaac tccacctacg atggtcttgc
     2821 cactgccccc gattcttctt ctgcagaaga tctcgccaag gcactcgtcg ctggcgcaaa
     2881 ggtcgtcaaa gccttcaaca ccacctatgc cggtacgctc atggctggtg aagttgctgg
     2941 tcagaagctc gatgttttca tcgctggtga tgatgccgat gccaaggcaa aggttgccca
     3001 actcgtgacc gatggcggtc tgcgcgccgt ggatacaggc cccttgagcc gcgcccgcca
     3061 gatcgagggc atgcagttac tgcacatcgt tctgcagggc tcactcggaa ccaattgggg
     3121 tagtgcactg aaaattttag gataatgtaa aatggagtgc ggggaagccc cgcactccat
     3181 ttattgtttg tctcatcggt tgacgatgac ttttagaatg atgagacact tatagaggaa
     3241 agaatgaacg cacaaacaaa tgccgccaaa acggaattct cgattcatcc cgccacgaaa
     3301 cttggtctgg tctcgttgac agtagccaac cttgagaatc agatcctttt ctatacccag
     3361 gcactcggat tcaaagttca ctggcgtgaa ggcaacaggg cgggccttgg tgcaggcggc
     3421 gcagacttgc tgctgatgac cgaagaatcc aacctgaaga gataccgcgg cgtgacaggc
     3481 ttgtatcact ttgcggtgct gttccccaat cgccgtgaac tggcacgtgc cgtggcccgt
     3541 ttgtctgtgt tgaaataccg caaccacccc accgaccaca tcatgacgaa gaccacctac
     3601 ctcgatgacc ccgagggcaa tggcatcgaa ctgtattgcg aatcgcccga agatggttcc
     3661 tttgtcatcg aaaacgatga ctttgtcacc cgccgtactg acggttcctg gagcgatggc
     3721 cgtgagccct tggatgtgga agccttattc tcccatttga aggacgatga ccgtctggac
     3781 gatccattgc ccgctgaaac acgggtggga catgtccatt tgcatgtgcg caacgtgcag
     3841 gaagccgtgg atttttatca cggcatcatc ggtttcgatg tgatggggaa ttcatccaaa
     3901 tttcaggccg cgttcatttc cgcgggcggc tatcatcacc accttggctt aaatgcctgg
     3961 aaaggtgaag gcgctcctcc tccccctgcc gatgcagtcg ggttgcgcca tttcacagtg
     4021 gaattgccga atcaacaagc attggatgag gttgtcaaac gcattgatga tgcaggcatc
     4081 ccatccaaca aaacggatca aggattgctg ctgtacgatc catcgcaaaa cggtgtcatg
     4141 ttgaaagttg gataagcaat tataaataaa gtatggacac agtaacgctg tgtccatact
     4201 ttattaaaaa gatttgcaga agatgaagat tatattggac ctcttgcata agttgcaggg
     4261 aatgtctttg aaaagccatt ttgaaccacg aagacattga agcagtgcgg agcctcctgc
     4321 ggacggagtt tttaattgtt ttctctgtgt ctccgtggtt aaattttcag acttttgcaa
     4381 gaggtctatt gaccatgtca atgattttta ctgatgtctt ccattatgaa ttacagggat
     4441 atcacggggc cagacaggca gggatcgaat cgcttgcgcc gtcgggaatc tgtgtagtcc
     4501 cgttctcgtc gtagatcttc acaaattgaa ttcggctgct gaattgtttt aaattcacca
     4561 tcagaatatc ggcgaggttg taatccctgc cagcggagtt gcaggtgcct ttgaggtaga
     4621 catgcgccac accttctttg acctcgaatt ttgaataccc cgtaaatccg ctgaacacgg
     4681 cggtccaacc gtagtcgttc ttttcggtca ggcctggacc tttgaaatat tcatccagaa
     4741 cgaattttgc aaagtcagct gatgaactgg cgtagcgttt gccttccact tcgaagggcg
     4801 gagtgttggc gttgtatttg gatttgttca caaagtacac ggtcactttg gtgtaggcgg
     4861 gcgttgccga ggggagtttt gtattcgttg gcgtgggggt acgtgacggg gtgcgggtgt
     4921 tggtgggtgt ctttgtaatg gtcggggtgc gtgtgaccgt gggtgtcttt gtcggtgtgg
     4981 gggtgcgtga tgcagtgggg gtgatgcttg cagtagccgt ggccgtgaaa gacgggtcaa
     5041 ggcagatcgg ctcggagtca ccggcgccag acggattttg agtttgatcg aactggtcgt
     5101 agatctttac agattgaatg gtgccaaact gtttcaagtt caatgtgatc aggtcggcaa
     5161 tgttgaaatc ttttccgctc gaaacacagg cccccttgag gtggacatgt gccacatcgc
     5221 caacgatctc cagtttgttg aaccctgtaa acccgttata gatggcggtg tatccccatc
     5281 catattcggt cgaaccaggg cctttgaagt attccttcaa aacgccttct ggtaaattgg
     5341 atgaggagga aaggtaacgt gtgccaaata cttcgtaggg cggtgtattg gctttgagcc
     5401 tgaaacgatt tacgaaatac agtttcactt ttgtataaag cggcgtggct gtggcgggct
     5461 tggtgtttgt ggcagtgggc gtcttcgatg gcgtgcgggt gatcgtcggt gtccgtgtga
     5521 tggtgggagt gcgggtgatc gttgccgtgc gcgtggctgt tggcgtgcgg gtgatggtgg
     5581 gggtgaaggt cttggtcgga gtccctgtca gcgtacgagt cggtgtgggg gtatcagtaa
     5641 caattgacgg cggagtcgga gtcgcgtcgc cttgatagat gaattgaacg gaatcagcac
     5701 ccagttccat atccgcgatc gcagcggact catcctgggt ctggttcgtg agtcggatga
     5761 attcgctccc tgtaccggtg aagttgtatt tgcccacgta gatccagcca tcggcggcgt
     5821 agaaacggtt tggatacaca gcctgattga tgagcactcg cgccgttttg cctgcgtgtt
     5881 gaatatcgta gaacgcacct tccaccgtgg cgtgattcga cggcacacgg atatagaccg
     5941 aatacattcc tgctgtgctg ccttgtggga attcccattg cgccgagcag gttgcggtcg
     6001 atgtgcgcgg tttgacgtaa cgcattgcgc cgttggttgc gttggtggca gtgcctgtct
     6061 tccaacatgc tgcgggtgat tcgctgaagc ctgcgccgcc atcatccacg acaatgccgt
     6121 tgacgggagc aggcgggtag cccaatgcac tccccgaagg aaagatggcc gcgccatagt
     6181 aaatgagcga tggatattgg acccacagtg attctttttg gttgctgggc cacgggtctg
     6241 tgccggagcc tgtccagccg taggggtcaa cggctttgcc gcttgggttg accacgcgca
     6301 aatgcaaatg cggacccgat gaattgcctg tgctgccgct gaaaccgatc cactcgccgt
     6361 gcgggataaa gacacagccc ggtgtggagc aggctgagac cgccactgca ctcatatgac
     6421 cataggaggt tgaataccca ttcggatgat gcaggcggac gtacaagcca aggctgacct
     6481 tgtgattttg cggatcccac cagccagaat agatgacctg atcggcatcg gcggcggcat
     6541 acagcggacg atagctcatg ccatagtcaa tgccatcgtg accatcgtag aagagccaat
     6601 cgccgagttt gtaagaccag tagccaccat agccaatatc acacacacct gcttctggcg
     6661 gaggtgtgcc cgcgggcggc gggttggggc aggtcttctt accttcggcg ccgttgaagg
     6721 tgacgacgcg gttatcagcg acataattgg gggaggtgtg gtcgaaaacc gattcttgcg
     6781 aagccgtgcc aggatatggc ggatataaaa atgcggtcac cgcctgggca ggcgcggctt
     6841 gtgcgcgctc ggccaatgac ccgcgcaagc cgaataaaaa caggacgaga aagattgcga
     6901 tgaaaatatt ttttgcgcga tgatgagtca caatgatttc cccaattcgg attatggaac
     6961 aggtgtggcc tgggtcactt tgaccgatgt gaatgtggca aataccattt cggcttcctg
     7021 caaacacagg tcggcgtttt cggcgaaggt tacttcgaag cccgtgtaaa tattcgtgtc
     7081 gccgccaagg taggtgacgg attgccgcac tccgccgcga aagacggtgt tgatctcaaa
     7141 ttcaaccaca ccaattttgc gaatgtcatg ttccacggtt gtgttggagg gcagtccgcg
     7201 gccaaaggct ggagcgatga cgcaatactc gatgccgcga tgaccgatgg cagggtagcc
     7261 atagctatcg gtggtgagcg cccaaatttc ggggtcgtaa cgcacgcgga acagataatc
     7321 gggtttcgaa tcgccgaggt aggtgggggt gccatcccat gcgagcaggg cgggcgggtt
     7381 ggcaagcggt gtgggcaatt cgagcggcgg caacggcgtt tcggttgggg tcattgcggg
     7441 gggcacgagt gtggctgtgg cagtttccgt ttcaagcagg gcggtggcgg tcatcgtggg
     7501 tggttcagcg gtcggcgact cgaattcctg ctgggcttcc actttttgcg cgggtgcaga
     7561 catggccgca ttttgtgcgg cacaggcagt gatgaaaaag atggagagga gggtgatgag
     7621 ttttcgcatt cagttatttt ttaccacgaa gggtcacaaa gatacacaaa gtttttgatt
     7681 ttcctttgtg ggtaaagatt tacttccgca tccaacttaa acagcaggct ggttctgcga
     7741 gaagggcctt gatctccgct gacagttgat ccgaaggcgc cgtttgcaat tccattttac
     7801 cattcaggac tcgcaggaaa aaaagcgcat ttccgtccgg tgaccagtgt gcggtttcga
     7861 ttcggccttc gctaaagttt gtgatctgcg tcaattcgtc tgttgtgaca tccacaacat
     7921 aaagattgct gatcagcgcg tcggcagaga cattcgccgc ttcatcttcc gcgttttcac
     7981 ggacgatgaa tgcgatgcgt tttccatcgg gtgaccaatt cgccgcgtag ccgtgacctg
     8041 catctgcgtc ggcaagtttg cgcgggttgc tgccatcggc atccatcacc cacaattcgc
     8101 ccactgtgaa cggggtctgc gaatcgggaa ttttgatgaa ggcgatctgc tttccattgg
     8161 gtgaccagcg cgcaaaggaa atgtaattga gcgggtcggc atacaaaagc tgaacgatgc
     8221 ggccatcgtc tttggcaagt tgaatctcgc tgccaaatcc gaatccgcgt gagtaggtga
     8281 aggtgaaatc aaagctttca ggtttgggag cgaggtcgta ggtgaattcg ttgatgggga
     8341 tgatctcagc ggcgagcgtt tctgcattca cgcgcaggat gcgtggatcg gaggcattgt
     8401 ccacgcgtag aaaaacggac ttgccgttgg aagcccatgc aagcaggtga ctgtcaccct
     8461 gaggaaatgc ctgggtgacc gaaccagttt ccgtatccag aaaaagaagc gcctgcccga
     8521 acgagcagga aatttccacg gcgagggtgg atccgcgtga cggggagaaa atgtcgtaga
     8581 tgccgcaatc tgcaggaagg gaaaagggca gttcgccaag gggggtgatg tcttcggaga
     8641 attggatcag cgcgggaggg tcaagccgaa tggcggcgag gatgtttttg gcgggcggtt
     8701 ggggcgagca agaggaaaga agtaagatga aagaaatcaa tgcagaaata tgggacttca
     8761 taagggtatt ataatcatcg cacatttgta aatccaaaat cgtaaatctc aaatcggaaa
     8821 tcgactcatg cacatacttg taaccaatga tgacggcgtg caggcgcccg gactcctcgc
     8881 tctggcgaag gaaatgcgta aacttggcaa agtgactgtt tttgcacccg ataagaactg
     8941 gtctgcttcg gggcatgtga aaacgatgga gcggcctttg cgtgtgcgcg aagtgctgct
     9001 tgcggatgga agccctgctt tcgcttcaga tggtgcgcct tccgattgtg tggctttgcc
     9061 gctgcttggc ttgatcgaaa agcaggtgga cctggtcgtt tcgggcatta accccaatgc
     9121 caacctcggc catgatgtga cctattccgg tactgtcacc gccgcgatgg aagctgtgat
     9181 cgcaggcgtg aagggaattg cggtttcgct ggattcacct gaggggttca aaggcaaatt
     9241 ggattattcc accgccgcca ttgtggcccg ccgcgttgct aaaaaagtga tggctgacgg
     9301 cctgcccgaa ggtgtggtga tgaacgtcaa cgtgccgtat ttgaagaagg agaaactcaa
     9361 gggatacatg atcacccgtc agggtttgcg cgtgtatcgc gatgcgctgg atgagcgtgt
     9421 tgatccgcgc ggcaaaccgt attactggat cggcggcgaa gcccccaccg gcgtggacga
     9481 gcccggtacc gattttggcg ccctgcgtgc ggggtatgtc tcgatcacgc ccctgcaatt
     9541 ggacttgacc cattacaagg cgatggatgt gttgaggaag tggaagtttt aggatgtgac
     9601 cggggcagag cctcatgacg caatatttct ttgtagatga atcgggagat gcgggtttgg
     9661 ctgggcaaat tggctcaagc agtcattttg ttgttgtaat ggtgcaactg ccagaacgta
     9721 caaaggtaaa cccgcttgtt gaacttagag attcactcaa cctatccccc cactttgaat
     9781 tcaagtttca ccagtctaaa ccctggcaaa aagaacgttt ttttaatgaa ctcttgaatt
     9841 tccctttcag gatccgtgcc gcaattgttg ataaatctca ggccgatttt cacccgcaag
     9901 agatatctcc ccgccagctt atcattgatt tgatcgttgg gcttaccatg agagcagacg
     9961 aattggatgt ttccaatgat attcttatcg tagatggggc caccccaaca ttctgtcgtg
    10021 accttcgcgt atcttttaca gaacaatgca ggcagaaaaa acgaatccgc ccttttaaga
    10081 acatcgttgg ggcaaattcg aaaaatgaag atgggttgca aattgcggat atggttgcag
    10141 gagcaatacg tttacattcc atcggcgcat tggatggata ttttcacacc ctgttgccga
    10201 aaatcgttga tttgtggcac ttcccggaga aataaaacac ccccgactat tctcttgcga
    10261 gaaagacagc ctcaggtggc taccttgtcg tcgggggcgg gtaaaacggt cttccatcca
    10321 ttgctgggtg cttccgtttt attggtaaac tcattataca gagaatggaa actgacgtca
    10381 aacccttgtt ttaggaaaaa aagaaatgga cttcttcaaa aaactcttta gtggcacccc
    10441 tgcaaaagcg caaaagcgct accatacctt ctctgtcaaa tgcctgcgtt gcggagaggt
    10501 ggtcgaaggc cgtattgacc tggacaatga actgagcgtg gaatatgaat cgaatcgcga
    10561 tgtgtattat ggccgcaaag tattaatggg cagcggccga tgcttccaac ggatggaagt
    10621 ggaattgaaa ttcgattaca accgtcaatt gctggatcag caagtgagcg gtggagaatt
    10681 tgtctaatgg acgaatcgat cgtagatcat tattggtatt catttttaca cagcctgccg
    10741 cctgattcgc ggtattttgg aaaactatat gtcgccgaac gttttggcga taactctgca
    10801 ctggcgcagg agctcggcca attggtggtg gacggggtca agaccggtac atgctctgcg
    10861 atttgggagt gggaggccga gggcaaatcc attccccagc ccggtctgat caccattgtc
    10921 cttgatggaa atgaccagcc catgtgcatc attgaaacca ttgaggtctt tgcctgccgt
    10981 ttcaaagatg tggacgagga gtttgcagag gcagaggggg agggcgatcg ttcgctggaa
    11041 tactggcgca gtgaacacaa aaagtatttt tcgcgtgtgc ttccaaaaat tgggcgggaa
    11101 tttagtgaag atatgccgct ggtctgtgaa cgtttcaaag tcgtttataa ataatgcgtc
    11161 gtctctcgaa cattctcctg atccttcttg ccgggcttct ggttgttcag gcgggcctct
    11221 ctttgcgatg gcccattgca cacgatgaag cgccgcttct ttacgaagcc ttcctcatgc
    11281 atagtgaagg ccgtgttccc taccgcgacc tttttgattt tcaaatgcca ggcagttttg
    11341 ccgcgtatta tttgctcggc aggttgagcg gcttcaacgg tttcttccgc ttgcgcctgc
    11401 ttgatcttgg catccttgcc gcgctgctcg ccatcacatt tttattcatg cgccgttttg
    11461 gcagacaaac cgcatttgcc gccgcgattc tctttgggtt gaaatacatg cagggtggcg
    11521 cgttcatgtc tttgcagcgt gaatacctcc tgcttggttt tgttgcactt gccgtttggg
    11581 tatccatgcg ggatgtcttg acctataaac atcgtctgct cattggaatt ctctttggcc
    11641 tatcggcagt gatcaaaccc cacgccgcgc tcggactcct ccccatcctg ctctttgaca
    11701 ttacaagcct gatccaacgc caaaagttga ctctgattaa gtccgccatg accagcattc
    11761 cccccgtcac gatcggattc gcaatgccca tccttgccat gatcgggtgg cttgccgcta
    11821 caaacgcgtt gactcctttt ctggatatcg ttctgcacta tgtgcccttg tacgcgcaga
    11881 tcaacgggca gatggagatc actgaagcgg ggacgagaat tccattttta ctgaatcaat
    11941 cccttcgtct gggcggacac gcgctttggg tgattccagc agcgcttggt gtgtatctcg
    12001 tcccgcagga aaataagaga caggcctggc tgcttgctgg tcttgctgcg tgttacgccg
    12061 tttaccctgc attctcgggt cagttctttg aatatcacta catccctttt atttatttca
    12121 ttgtcatact tgctgcgctg gctctcaccg aacaacagcc actttccact ttccactcac
    12181 tgccttcttt cctctttcca ctttcttctc ttatcctttt atttgccatc gctttcgctg
    12241 tccgaccgtc caataccttt ctccgtcaga tccacaaccg acccattgcg aaaaccacag
    12301 atcgagccga agagatcgcc agttatcttg aagaaaatat gcaggcgggc gataccgtcc
    12361 agccattgga ttggacaggc ggcaccctgc ttgccatgct tgaaacgcgc gcaccgctgg
    12421 ctacgaaata tgttttcgat ttttattttt atcatcacat ttcaacacct tacatccaaa
    12481 acctgcgcgc cgatttcatg gacgaactgc aggaggccga tccgcgcttt atcgttgagg
    12541 ttatcgccgt ggataaaccc tggcttacag gcccggatac ctcccgcgaa tttcctgaat
    12601 tacgtgcgtt tctgaaagca aattattccg ttacaatcct tgaagacgac tatttgattt
    12661 ataaaagaaa gtagtgcagg cttaaccctg ccattccccg cagcggacca gagtccgcgc
    12721 gttgcagcga aaacatgaag catcttttcc gcatcaccta cctgtacctc accatcccat
    12781 tcatcatttt ttgcatgggg tggctgcgct tgtctgttgc gattcctgtt gtggctgtca
    12841 ttgtgtgggc gctctggcaa ttgctaaaac aaaccccgcc tgggcaagat tcgaaaatta
    12901 ctattcacca tttactactt gctttactgg tcactggcat ctggctcttc ctttccgggg
    12961 tgggtggata tgcctttcaa aactgggacc atcactggcg caatgcggtc ttccgcgatc
    13021 ttgtgacgaa caactggcct gtgatctatt cctcccccga gcgtggaccg atcaccatgc
    13081 tggtgtacta cttgggcgac tggcttcccg cggcgctttt tgggcgggtc tttggctgga
    13141 gcattgccaa tttcatgctc ttcctttgga cgtggcttgg ggttctgctc accgttttgc
    13201 atttgaatct caaactgaag acctcgctgg tcaggtccgc attgctgttg atctttttca
    13261 gcggattgga tgtggttggg gcgctgcttt ttgcaaagga atacccaacg ctttggccgc
    13321 cgatcactca ccttgagatc tggtcgggtg gtgatttgca atactcctcc ttcacgacgc
    13381 agttgttttg ggtgttcaat caagctgtcc ctgcgtggct gtgtattgtg ctgatcctgg
    13441 gtgacaatgt cattgcgagg agggtgcttt ccccgacgac gcaatcctca attcataaag
    13501 agattgcttc gggcatagag caagatcgcc ctcgttatga catcaaattt ttcatctggg
    13561 cgttgtcatt tttctttgcg ccgctggcat ccattgggtt gatgccctac ctcatggtcg
    13621 attttttcac gcaggccgat tggagatcgt ttttcaaatc cattcgtgtt gaccttttgt
    13681 tcgctgcggg gatcatcttt cttctttcgt attttttctt ttcctccaac accgccgcac
    13741 aggaacgtgg ttttcactct cttgtgctga aggattggct tttcttcttt ttgcttgatg
    13801 gcggcatctt ttggttgatg ctggctccat tcaaatggcg cgatccgcgc tgggcggtga
    13861 cggggattct gcttttggtc atgccgttca ttcaacttgg caacggccgc gattttgtga
    13921 tgcgggcttc gatcgcgccg ctgttttatt tgatgatgat ggttggcgaa acgattttta
    13981 aaaacaccac agcccgcggt ttgcgatttg cgttttatgt tctgcttttc ctcggcgcgt
    14041 tgactccgct ttacgagatc aaccgctcgg ctgtgcggac ctacgaatat tattttgtgc
    14101 tggatgaaac ccagcgcgcc caaccgaacg ttgaacctgc cacacatctt gaaaagggtg
    14161 gtgcgcctga gcttgagcat cccaatgcca tccttgccga tgatatttac acgctcacat
    14221 ttatgcagga taaactctcg aagaatttca ttgccaatgt caggcagtct ttgtattatc
    14281 gatacctgtc gtcgcgttga tctgatacct aaaaaaggaa aattcccatg acccagcaat
    14341 tcccgtccct tccgaaccct caggcttttt atgccgaggt gtggaatatt gtctgtctga
    14401 tcccgcgtgg caaggttgct tcgtatgggc agatcgcgaa aatgatccgc ccgccagcag
    14461 gtgtggaccc tgaaacctat ctcgaatttg gtgctttgtg ggtcagcaac gccattgcgg
    14521 ccagccccaa tgaagtgccg tggcagagag tggtcaactc gaaaggtgag atcaccgagc
    14581 gtgatgggct ggaagccaag cgccacaaac ttttgcttga ggatgaaggc gttccgtttg
    14641 gcattagagg gcgtattgac atgaagaaat atggctggag tggaaagtcg aaatagacga
    14701 caggccacga tcactggtca acggtttact gtcgaaagga taaactcatg ccttccttct
    14761 cctctccccc caacccacaa gcctattacg aacaggtctg ggcgatcacc cgccttatcc
    14821 cgcgcggaaa agtcgccacc tacgggcaga tcgccaaaat gattccgccg cccaatggtg
    14881 tggaacttga agcctatgcc gcgttcgctc cgcgctgggt gggcggcgcg atggcgaact
    14941 gccccgacga tgtgccgtgg cagcgcgtga tcaactcgca aggtaagatc agcgaacgcc
    15001 ccggcgcgga gaggcagcgt ccgctgctcg aagcagaagg tgtggtcttc gacgccaaag
    15061 gtaaagtgga tttgaaaaag ttcggctgga gcggaaaaga cgaaagcgac atcccgcaac
    15121 aaacggcgat gttctaaata caaataaaag aggaaagaag aaagaaatcc tccaccactt
    15181 ctttcctctt tcttcattct ttttcatcct tcctcattca tccttcatca ttcaaatgtc
    15241 cctcctcatc atcggcggca cctccaccga catccttcat cttgaatccc aaaccgtccc
    15301 ttctgcgggc ggcgcaggga tgtacactgc catggcagct caccgctgcg gatcgaacgt
    15361 ttcacttttc gcacccaagc ccaatcccat ccccgatgtt ctccaaccca tcgcggacag
    15421 actcacagaa tggtttggtc ctatcgttga ccccgaagat gggctgccgc attttgaaat
    15481 ctcccaacgt ggcggcaaga ccgaatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnncccg aagatgggct gccgcatttt gaaatctccc aacgtggcgg caagaccgaa
    15661 tatctcaaag cgaccttcgg cgcagagtcc acgctcacgc cggatatgct gccaaatgat
    15721 ctttcaaaat acgactgtat tcatgttgtc ccgctcggcg acgcgcaaaa gcaactggac
    15781 ttcatcaccg cctgtcgcgc acacggcgca aagaagattt ccgcgggtac ctacattgca
    15841 ggcgtggaca aatctccgca cctcgttcac gacatcatcg cgcagaccga tatcttcttc
    15901 atgaatcaaa gcgaagccat cggcttgttt ggctcacttg aagcggcgca tactgactcc
    15961 ggcaaactgc tcttcatcac acttggcgca gatggcgctt gcatcattca aggcgatcac
    16021 aaaaccttca tcccctccgt acccaccacc gagctcgacc ccactggtgc gggtgacacc
    16081 ttctgcggcg cgacgctggc atatctagcg cagggagcgc atcccatcat ggcggcacag
    16141 cacgccgttc cactcgccgc gcagatgatc gcgcatgttg gtcccgccgc gttgttgttc
    16201 aacgaacccg cgcctgagat tccagccagc cccaaagcgc agacgaaccc aactcagatt
    16261 caaaaaattt ccaaaatggt tgccgcgctc gatgaagccg cgcccttcaa cttcatcagc
    16321 cccgagcttc cgcctgcgca tcaccccaag gcgctcgatt attttttcgc ggtcacactg
    16381 cagcaattca gtttttggaa cgaacagaac aatcgctatc acacgccgct gattgcgccg
    16441 cttggcggag tcagccaaaa gggcgcattc tatttgttcg acgcatacag gcgtcggctt
    16501 gagtcggacg cggatttttg ttcccctgag cggcaggcaa atttgagtcg cgccgagctg
    16561 ctcgagatct tcaaagcgga tgatggaacc gatcccatgc ccgcactgga tttgcatctg
    16621 gaaatggcga atgcttacgg gcgggatatg cttgcgcttc atctcacgcc ggaagctgtg
    16681 ttgcaaaaag cccgagcctc agccaacccg ttagcgactc tcatctccat cctcgacaaa
    16741 attggcggat acaaagaaga cccgctccgt aaaaaaagcg gactgctcgc gctcatcctc
    16801 aatcaacgcc ctgaagcatt cctgaccttt gccgctggcg agcaagtgga accagtcatc
    16861 gactatcacc tcatgcgctc atgcttgcgt gtgggtttgg tggatattct cgacgaagat
    16921 ttgaaaacca gaatcaccaa ccgtgaactt gtctcccccg ccgaagaatg ggcgatccgc
    16981 cacaccgcct accgtgccat tgaagaggtt gtggcattgt ctggcaaatc caccggcgcc
    17041 gtggactggt tcttcttcgg cgcgcgcaaa cgttgccccg aaatgaccga acccgaatgc
    17101 ggtttgtgcc agatcgattc ggtctgcgcc caccgcaagg aattgtttca acctgtgtta
    17161 cgaacgacat tttattaatt gaccgtggac agcgaacgat agaccatcat ccaaaaactt
    17221 tatcaaggag ccataccatg caatcccccg aaaaaccgac caaatttgga accatctctt
    17281 tcattcttgc catcgtcgtc gttgtgttgt ggtgcgttta cttcatcctc tttgctgcaa
    17341 tgaccgaagg tggtgcaacc ttcggcatgg atggtgaaac tgcaggttat gcacttgttc
    17401 ttggcggcgg cacagtgatg gcagtgctga ccgttttgat cacccttgca ggaatcatcc
    17461 tcggcattct cgcactacgc aagcaagacc ccaaacgcgg acttgccatc accgggcttg
    17521 tgctcaactt cttatgcttt gcgccctatt gtcttttgct gttgttgatc gcctttggca
    17581 gtatctccac tgccgatttc attccctagt atcctttcaa ccagttccca aaatacccag
    17641 agccaggttt ggaaaaacct ggctctttgc ttcttatcca tccacatcct gcaaatcact
    17701 ttaaaagtac gatgtctttt atgagagtgt ttttataagc gaagggctct aattatggac
    17761 atcgagattc ttcgctttgc acaggatgac acttttgaga agcattcgct aaaaacgaaa
    17821 gctctggggt aaaatgccgt ttcaaaaaaa taaatgagga tgagaaaaat aatgagaaaa
    17881 tggcgtggga ctttctatgt tttgtttttg gctgttctga tgatgttact ttcaagttgt
    17941 gctcccaagg tgacacttac ctatcgtgtt agcggaacag ccaccaaggc tttggtggaa
    18001 tatcgcgggg tggatgggga gaaaacacag gaaagcgtaa cccttccttg ggagaaaagc
    18061 atggaagtcg gggaggattt ccgttacgaa ttgtacgtaa tgaacgaaaa tgaaggtgga
    18121 gatatccagt gcgagatttc tgctgatgga atatcgttcg gtaccgctga gggatccatg
    18181 tacactggtt gttcaggggt tgttagaact gatgggaaaa cgacttcctc cttcatgacc
    18241 accctgaatg atacctatcc agatttgagc gatctgcctt ataaggaaca ggggcgtttt
    18301 ttgatttaca cctcacaaaa gtttgatatg ggcgagatcg tgataaaaga cttgaaaacc
    18361 ggtgaagaaa tgcaaatgac ggacggcttg agaggtggat actgtctttc aaaatctcct
    18421 gtgaacgcca aggtcgcgtt cacctcactg cgggatagcg tcaatggcga tatttatatt
    18481 ttggatataa acgccaggaa gggtgaagaa ctaacgaaca ttaccaacac tcccgatacg
    18541 tatgaaatgt gcccctcctg gtcgaatgat ggacagatgg tggtattcac gttcaaatca
    18601 tctgaaacct ccccgtggga gatcggcaag atcggctact atggtggcga tgtttcacag
    18661 atcaccaact ccacagcggg tgagaattcc ctttacccgg cctggtctcc agatggagca
    18721 aagatcgcct acgtggtcaa taacgaggat ggcgacactg tctatgtgat gaacgccgat
    18781 ggcagcaacc ctgccccgct ttcggaaaca tttgccagtg ctttctcggt cagtcaggtg
    18841 gcgtggtctg tagacgggca gcagcttgcc ttcttccagc acaatgatga ccttgtaagc
    18901 ttattcatcc tttctgcaga tggaaacgtg gaaaagagaa ttgagatgga tgtggcgcgg
    18961 acaggctcac tttcatggga tgccgaagga aaacagatcg ttttccatgc ctggaagaac
    19021 atcagcgagt cagcagatat ttactcgatc aaccttgagt caggagagat aatgatcctt
    19081 gcagaaaacc ccacgttgga ttttcttggc ccgttcctta ataccgatga caccatcacg
    19141 tcgtttcccc gccgtacaat tgaactggcg caatagtaag gttcattcac cctgttgctt
    19201 tgtcgcctgc aagcgctgaa aacggagtat gtgcatcccc cagtttgcat cggctgactt
    19261 atcgtgccag ttccaccacc aatgtatcaa gagccaggtc cagcgtgatc tggctcgttt
    19321 tgactctctc gtcaatgccg agcagacggt gataaatgct ttcaagcgat tccatcgtaa
    19381 aacgcgcagc ctgtcccgtt gtcttttcag cgacaaacgg atgaaccccc agcgcgcgcg
    19441 ccacgtcgtc cttgtttccg cgcccgtcca gaatttcacg cgcctgaatc aacaagcgga
    19501 attgacgcac gaccatgccc cacaacgaaa atggatcttc gttttccaaa agccgatgca
    19561 ataatttttg cgcggatttt ccattgccct gagacaacgc atcgacaaaa tcaaacacac
    19621 tttgctggga tgtgacgata caaaccgctt ccacatccga cccgtgaacg ggtctggccc
    19681 aattgacata ggccagcaat tttgagagtt ccatccccgc ctggcgggta tccacgccga
    19741 ccatttctgc cagccgtgcc gccgcaggcg ggtcaatttg cccgcccaga ttttttgctt
    19801 cattaatgat ccagcctggc atatctcttt gcttgggtaa aaagaatccc aacggtttga
    19861 cctgtggctt gttcttttct gcccattttg tcagccagtg tttttctgct tccttgggtt
    19921 caatggtttc aaaaagcacg attttggcag tatcgggggc tttttccaga aattccaaaa
    19981 acttcttccg tgtggatgga ttgttgaatt tccccgatgg ccctgagata aagaccagtc
    20041 tcttgggggc aaggaagggc atggcattga ccgcattgtt aagttcgttt tcggtcatgg
    20101 tccgcgcttc gagacgggtc gtattcatgt ccgcggtggt ggggtcggtg aaatccgact
    20161 cgaaatcttt gagcttgcgc gagatcgcaa actcgtcatt gccatagagg aggaagatgt
    20221 tgggcattgg agttgaaagg cggaactttt gtgctttgag ttagtgtttc gattgctttt
    20281 gaacgatgat gcgttcgctt tcgatgcgct tgttgatgaa ttcgtaaaat tcttttttgc
    20341 cttttcctgc gatctccatc gaaactgaaa gatgcttttt cttgcctttt gtttgaatgg
    20401 tgaaaagctc gcctttcagg gtgatgattt ccacatcgtc ccaattgatg gaggttgtgc
    20461 cgaatggcgc gtgcaggctg atggacgaat ggttgacttc aatggctgag aaggcaaaag
    20521 aaacaaggaa ccacaggaac acaagcagaa aaacgatctc aatgctgcca gcaacggaaa
    20581 tcgccgcaaa cattttggtc aaaagataaa taagccccca aattacagga atgcataaaa
    20641 ccagcaggaa aagctgtaac gcccaatgaa tattaactct aaagttggtt ggggtttcgg
    20701 tggacataaa ctggattcct ttttattttg taatcacccg atgcacacct ttgcgaatgg
    20761 ggatgatgtc aatgatctgc atgttcccca tgttggcttc ggtggtgatg cgctgctgca
    20821 gcacagggcc aagcggacgg gagatgagga agccgacaac gccgaggaac acagccagcg
    20881 gcatgagcag gatcttcatc agcgtggatt ttgccaatcg aagcaccgac caggcaaagg
    20941 tcccggctag aagcgctgtg gtcgccatgt tggtcccaca accggcatgg atcgccagtc
    21001 cgctttcacc gccgcgtaat ctttccagtg cttcagtggc ggccgcccaa acatcctgcg
    21061 tggaaaaatt cccgaacagg aaaaagccag tggggttgga gtgccctgcc atggttccag
    21121 tgtgagtgtg tgccagcatg tgaagcgtgg catgttcgag cgcatggttg cgccgcgttt
    21181 caaggatgaa gggaaggtct aggatcatgg tttgattata cgttaaatcg atacgctcca
    21241 atcggggtcg cgttgttagg gtatggcagg ttgggggatg atcggcgagt cgtaaatctt
    21301 ttcgagcaat tccaggaatt cagccgaggg gcgggttttg aggatctctt tgcgttcatc
    21361 gcgggtgagg tgttgcatca tgacatattg gaccgcgttc ttacggaaga gacgcgaacc
    21421 atcttcatcg ccgtagaatt caacacagcg ggcaagatgt tttcggatgg tttgtttcaa
    21481 catttcaggc ggaacctggt cgcggtcaag acgcgagaat atccacgggt tggggattgc
    21541 gccccgcccg atcatgacgg catctacatt ggtatgcgct ttcatgcgat caatatcggc
    21601 gacggttttt acatcgccgc tgccaatgac ggggatcttg accagtgact tgacctcggc
    21661 aatggcgtcc caatcggcgt tgcctgcgta acgctgttct ttggtacgtc catgcacagc
    21721 aatgagtgaa ccgccttctt cttccacaat gcgggcgatg agcttgtaat tttttttgcg
    21781 atcccaccca aggcggatct tgcccgtgac cggcacatgc agatgtttga ccagtttgcg
    21841 gaaggtgcgc gcgattttga tcggcgtggg catcatgcca acccctgcac cacggtccgc
    21901 aatggttttg gcggggcagc ccatgttgag gtcgatcaca tcaggattat tttcctggat
    21961 ctgaagggcg gctcgcaaaa tgagatcggg gtcatcgccg tagatttgaa aggtgatggg
    22021 gcgttcgggt tcggtgaagt acatccgctt ggcgggctgt ttggaacggc tgagaatttt
    22081 ctccactttg atgaattcgg tgtagctcat ggctgagccg agttcgcggc aaagggagcg
    22141 gaagggccag tcggagtagc catccatggg ggcaaggatg gtatctccat gaatgggcac
    22201 ttcacggatg tggaaagagg gtacttgaag aggggaggct tttgtattca ttttccgttc
    22261 tggaaagtat aacagagtat gttttggctg ttttttctgt taatgggcta ttttgtattc
    22321 attgagattt cgttttggtt ttggtaaaaa ggtactactt tcatttttga aagatataat
    22381 gtcttttaag tagacaagtt ttgtattgtt tttattgagg agagaaagat gaacagaaaa
    22441 atcctggtag ccattttcct gctgattttt acctttgcac aggttggcaa cgcttcagcc
    22501 gccactgtag aaggtacggt cacccttgta gaactaccca cattcagcaa tatattcgat
    22561 gtcgaccttg agtggagtgt aagcggaatg tcgggtgggg caacccagaa catttttgtc
    22621 tggggcaggc agcaagaggc tccgtccgtg gcttatgacc cttccgattg ccgtttggcg
    22681 gcctctttgc cgggtgtgag cgacgctgct ggcacttaca cctatcaata cttcttttcc
    22741 gctttcgatc agcaaacctg ggaatttacg ttgacggtgg acgatgctgt agattgtaat
    22801 tctgccacct taccctccac caattcggta atgggcagca ctttcattga tggctggtgg
    22861 ttggatgggt ttatcgccaa tccgccctcc cttgaccccg cttttttcga agagatcccg
    22921 gtggcctgca acaccttcga actgtggggg ttggtcaccg atagagtgcc agaccgtgta
    22981 gcgaaaacgc acggtggaga agcctcaacc cttggttatt ctggctttgc ttcgtggaat
    23041 caatccatta ccggcacctt cactccgcct gcacctttag ggccgccaga tgccttgctt
    23101 tcatgggtgt acaccttccc atcaactgcc agtggtctgt ggtcattctc tgtagatccc
    23161 accgattatg ctggaaacaa ctggggtacc accaacttcc gctattcgtt catcagcgaa
    23221 gaggaattag ctgattgtgt aaccttctca gatgtaagtg aaaccgatac tgaaatttac
    23281 gtccgctata tggccgatct tgatctggcg catggttatt tggatggtac cttccttcca
    23341 gatgccactt tgacccgtgc tgaaatggcc gcatttattg agttggccaa tggaatccag
    23401 ggtgatgacc ccgaatttgc gaccccaccc ccatctggct cggcttgtga ttttagcgat
    23461 gttagcagcg atgattggtt tgctggctgg gtttggcagg cctgtgctga tgggtatatg
    23521 aacggtatcg gcaacggttt atttgacccg aacaactacc tcacccgtgg acaggtggta
    23581 acggtcctcg ataacatcaa caaaatgggc acgggcagtg gttaccttgc cacccagaat
    23641 gtttttcagg gccaatgggg caacctgtac cgtgaagcag tctttttgga tgtcgctgtc
    23701 ggcgatttct acgcaggccc ggttcagcgc gcttttggcg ttggtgttgt ggatggaacc
    23761 agcgacacca cattctcgcc agatcaggcc attctgcgcg gcgagttcat caagtggatg
    23821 taccgcactt tgagccgcat tcagcctggc tgtgttgagt gccagtaata gggaaaagaa
    23881 tcaagaggcg gacccatttg ggtccgcctc tttttgttat gcaggctgct tttttagcaa
    23941 ttccagataa tgctttcgaa acttcgccac ctttggcgca acaaccgcct gacaatacgg
    24001 ctggaactgg ttccgcacaa aatactcctg gtgataatcc tctgcaatgt ggaagttgtg
    24061 aaccgcttcc acctccgtaa caatggggct gctccagatc ccctgggtat ttaactcgct
    24121 gatagcctgt tcagcagccg ctttttgttc gggggtgtgg taatagattc cagaacgata
    24181 ctgtgttccc acatccgcac cctgacggtt aagactggtg ggatcatgga tcgcaaaaaa
    24241 gacattcaaa acatcacgga atgagatgac cgttgggtcg aatttgatct gcaccacttc
    24301 agcatgtcca gtggtgccgc tgcaaacgtc acgataactt gggttgggaa catgcccatt
    24361 cgaatatccc gagtcgaccg aaagcacacc tttcatttca tcgaaaacag cttccaaaca
    24421 ccaaaagcat cccccagcca gtgtgatggt ttcataattt gtgttcatga taaaaactcc
    24481 tttttattgt ttggatcaca atgacaggcc tgtctgctga atatgacgcg tcgaagactt
    24541 gaaatattct taaatgcata tgatagttta agaaaaaaag ccgcccaatc cgtgggcggc
    24601 tttttgtgtg atcggtttat cgcatcccgg caactgtgat catgccgctg ctgatattga
    24661 tgttcatgat gcgcagccca ggcacctgcc tgttgatttc atccagcagc atctggttga
    24721 gccaggattc agcctgggta accagcacat cgggaatgac ttgctggccg atttgcatcg
    24781 attcgatttc aaagcccgga agtccgttcg catccaaact aacactcccc accatgagcg
    24841 cagacgttga gtcagcgctg cctttcacca ccccccaaat ctgtattttc ccgtcacgca
    24901 aatagacctg tacatcctgc aatggaaggt cagggctgtt tcgcatttcc actgcaagcc
    24961 acgaggtgat ctgttcttcg cttagtgtgg ctgtgttcag ggtaccgggc tggggcaggg
    25021 cgctttcaaa tgcttccttg gcttgtgagg cagccgtgtc tgaaggcatg atttcggcac
    25081 cggggcgctc cggggcgccg accatgccgc tttcggcatt gctgatcgca aagctgatca
    25141 acgtatccac caatggcgca tactcaggat atttttttac gatatctgca cgggtctgtg
    25201 gagcgacatc tccagccaaa gtttgggtgt agggtgtggg ggaggctgga ataattaact
    25261 tcgccatgtt cgactgcggg tttctactgg caatcgcaaa cgtggttgcc ccaagcccga
    25321 ttaagacggc caccgcaatc attacaccgc gcaatacatt acgtgctggg gccttggccg
    25381 ccgcagcttt ctgttgctga taagtggaaa caagcagggg ttgtgttttt gactcgagac
    25441 gggcaagggc gatctttgct tcattattcc ccgggttgat tttgagcacc tttcggtaac
    25501 actcaacctg tcgctctctt tcctctacta cacgtgcaag cagcatccag gcgggttcat
    25561 cttcgggatg cagggtgagc aactcaacga gatatttctt cgcgagttcc ctgtttccgc
    25621 gctggaagtt gtaatcagct aatttgagaa gttcttgttt gttcatggaa agccgtaggg
    25681 acacggcagg ccgtgtccct acattaggtt ttatttctag acgctcggct cgggttgttt
    25741 ttccgcggct ttgtttaata caacttcgcc gtcgtcattt acatctacaa aaatagtgtc
    25801 accgttgatg aactcaccgg ccagcaagcg gtcagagagc gggtcttcca cgcgctgctg
    25861 aatgacgcgg cgcagagggc gcgcgccgaa ttcagagtcg tagcccatgg tggcgaggga
    25921 ggaaagcgct tcttcagaag cggtcagaac aaggttgtgt tccaccagac ggtcggaaac
    25981 ctttgaaagc tcaagagaaa cgatcttctg aatatcttcc ttgttgagcg agcggaagat
    26041 caccaccgaa tccatgcggt tgatgaattc cgggcggaag gcgcgcttga gcgactcgtt
    26101 caatttcttg cgcatgtctt cgtaggacag gcgttcctcg gtggcttcat cgcgtttcat
    26161 tgcaaagcca agggaggttt gatttttgat catgtctgcg ccgatgttgg aggtcatcac
    26221 aatgatggcg ttgcggaaat ccaccttgtg gcctttcgca tcgctcaagt gaccttcttc
    26281 catgatctgc aggagcatgt tgtgtacttc agggtgggcc ttttcgatct catcgaagac
    26341 gacgatcgag tacggcctgc ggcgcaacgc ttcggtaagc tggcctgcat cttcatagcc
    26401 gacatatccg ggaggggcac ccaccaaacg ggcggcggtg tgacgttcca tgaattcaga
    26461 catgtccagt tgaatggcgg cgtcttcact gccaaacata aatttggcga gcgatttggt
    26521 cagctcggtc ttgccaacac cagtggggcc aaggaacatg aatgaaccga tcggacgctt
    26581 cgggtctttc aacccggcac gggcgcggcg gacggcgcgg gagatggcga tgatcgcatc
    26641 ttcctgaccg acaatgcctt tgcggagttc atcttccatt ttgagcaggc gctgtgattc
    26701 ggcctctgca agctgcataa gcggcacgcc cgtccacatg gagacgactt cagcgatatc
    26761 ttcagcagag acgacagggc ttgtggtgcg gtcccaaccg gtgcgcaggc gttcgatctg
    26821 ctggttgaga tcgagctcgc gctcttccca ttccttgatg tcttcggagt tgccttcttc
    26881 ctgagccaat gtgcggtttt ggcgtgcctg acgcaattgc ccgaacaggt ctttggcgtg
    26941 tttggcggca gggctcttgt acatgcgcac acgggaggag gattcgtcga tcaggtcaat
    27001 ggccttatcg ggtaaaaatc tctcggtaac gtagcgtgaa gataaatgtg ttgcggcttc
    27061 aagcgcttcg tcggaaatga caagatggtg gtgttcttcg taggcgctgc ggatgccctt
    27121 gaggatctga atggtctctt cctcagaggg ttcgtccacc tgcaccgatt ggaagcggcg
    27181 ctcaagtgca gcatcggatt cgatgtgctt gcggtactcg tccatggtgg tggcgccgat
    27241 gacctgcaat tcgccgcgcg acaatgcagg cttgaggatg tttgccgcat cgacggagga
    27301 accagcggct cccgccccga ccagcatatg gacctcgtca atgaagagga tcgcgcctga
    27361 ttgttttagc tcatcaataa tgcgtttgag tctttcctcg aactgaccac ggtacatggt
    27421 gccagccaca agggatgcaa catccagttg aagaacgcgc ttgttcatca acggagcggg
    27481 gacgtcacca tccacgatgc gttgggcgag gccttcaacg atggcggtct tgccgacacc
    27541 tggttcaccg atgagggcag ggttgttctt ggtgcggcgc gccagaattt gaatgacgcg
    27601 ttcgatttcc ttttgacgcc cgatgacggg gtccagtttt ttctcttctg ccttcgaggt
    27661 gaggtcggag gcgagttggt caacgagagg ggttttgggg gaggcgtttg cggcgccacc
    27721 tttgcccggt tgcgggccgg caggcgtcgg ggaggaggag gaagcggatt cgttcaggac
    27781 gcggcgagtt tgcctgcgga cctggtctgc tgtgacgccg agtcggcgca gggcttccaa
    27841 tccagtagat tcaacccgca cgagggcgag cagaatgtgt tccgtgccaa tgtagtggtg
    27901 gctgaggcgg cgcgcttcat ccacagcaaa ttcgaggact tgctgggttt ccggcgcgag
    27961 ttcgactcgg ttggggtcga aattgaccgc actgcccgag acgcgttgaa cgacttcacg
    28021 cacacgttca ggggtcatgc caaggtcgcg cagaacacgt ccggctactc cgccttcttc
    28081 atccattaag cctaagagaa ggtgttcggt tccaatattg ttttggcgag cgcgctcggc
    28141 ttcttgatgt gcgaggctga gtacgcgcct tgctcgctgt gtaaatcttt ccatgccagc
    28201 catagttttc tcctatatcc taaggcattg taccaaaaag gcgagggact ctgcgtagga
    28261 aaaaggttag agtttgctta cgggttttga gtgcaggtag cccttgcagc ctgaaatttg
    28321 gattatttga taatcagtcc tcattttggg ctggaatctt accgtcagga tgcctataca
    28381 a
//
LOCUS       DAOQLC010000053        28204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_539_length_28204_cov_26.297737, whole genome shotgun sequence.
ACCESSION   DAOQLC010000053 DAOQLC010000000
VERSION     DAOQLC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_539_length_28204_cov_26.297737"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PL000_10865"
     CDS             complement(<1..656)
                     /locus_tag="PL000_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNC89447.1"
                     /translation="MAAKQIVFSEDARRKLKNGMNVVAEAVSATLGPKGRNVAIDRKF
                     GSPTITHDGVSVAKEIELEDPFENMGAQLLKEAAQKTNDIAGDGTTTSTVLAHAIVNE
                     GLKALEAGYNPMLLKKGIELATETIVTELKKNSVKIDTREEIASVATNSAADVEVGEL
                     IADVMDKVGKDGVITVEESKTMQFETEFVEGMQFDRGYLSPYFITNGDQMEAQISDAY
                     V"
     gene            complement(689..985)
                     /gene="groES"
                     /locus_tag="PL000_10870"
     CDS             complement(689..985)
                     /gene="groES"
                     /locus_tag="PL000_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC89448.1"
                     /translation="MAKLSFKPLGGRVLVEPIEQEEVTAGGIVLPETAKEKPQQGKIL
                     AAGPGDRNDKGERIAMDVKVGDVVLFAKYSGTEVKIEGKKVLILRESDILGIVG"
     gene            complement(1200..2630)
                     /locus_tag="PL000_10875"
     CDS             complement(1200..2630)
                     /locus_tag="PL000_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC89449.1"
                     /translation="MNIYQRRDIFRKRGEINIYRMFFWTILILGGIWLIRSVQSGDVK
                     PLFYPTPTPTRFALSYALEGDAQFTAGELDAAITAYGEAAKVDPSNAKLLADLARIQT
                     YSSAMMVSDADTKVRLAEALASAEKAVAISPDDSYAQSVYAFVLDWNANPVLVPDERE
                     VQRLLTKANGAAVRALQLDPNNTRALAFYAEILVDQQKWTNAQQIIDQALERDPSLMD
                     VHRVHAYVLESLGEYALAIEAYDRAILIAPNLTFLHIRAGANYRILAFNSPNEEVQTQ
                     LFEKSLEYFDRAATINDQLGINDPAPYLSIAKTYSQMGQFFIAIRNVQKTIEFQPDKA
                     EFYGELGVLYHKNRNYETGILALKCAIRGCNPDESCEGRGGCGGGDVPAEVSGLPLTS
                     NTVYYYDIYASELAALSTPSKSYCGDALDIADEIEASEFDNDPNIAADIVVVRTICST
                     LNDAPAAGESPAATPETLDVTPTATP"
     gene            complement(2627..3877)
                     /locus_tag="PL000_10880"
     CDS             complement(2627..3877)
                     /locus_tag="PL000_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC89450.1"
                     /translation="MNRRRTRPNWFRIILLCLLVLGASYVNRFVIAARPSPFLPTPTV
                     TRSPESFVTEAQEYFKQGQLLRAIDSYKQAILSRPDDPTVYIALAQAQVFAGQYEAAQ
                     ESAESAILLNNANSMAHAVLAWALGAQEKFIEAEDSIERALTLDPNNALAHAYYVEIL
                     VDSGSFDVIEKAIDESRIAVALAPNTLETHRARGIILEAVGDYENAIREYQQAIDLNP
                     KIADLYLSLGINYRALGSYEDAITAFSNADALNPEDPTPDFLTSRTYATRGEFGKAMQ
                     YAEQAVANDGADPSLRGNLGVMYYRNGYWTEALVELGYVVDGGFTKDGVQIEAMPLVP
                     NNIRLAEYYFTYGLALARTNKCGEALNIARTILERIPADELSVENANAIISKCEQNLV
                     QTPEPLPTSPETAPVSTETPTPTP"
     gene            complement(4159..4230)
                     /locus_tag="PL000_10885"
     tRNA            complement(4159..4230)
                     /locus_tag="PL000_10885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4196..4198),aa:Lys,seq:ctt)
     gene            4604..5050
                     /locus_tag="PL000_10890"
     CDS             4604..5050
                     /locus_tag="PL000_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89451.1"
                     /translation="MSNSSNGVWKTFFGCGGFSLFLLTVMLLAVVLACSATVAVPIFD
                     RDVLQEIACPPGSTLITGWEETTYTRPGERVLYGYCVDAQGNESPAQDLGYGALEYFP
                     RYFGISLAASLVLTLLVVIPLMILYRVIKKKFFSKPVPTDPAIGSL"
     gene            complement(5100..6545)
                     /locus_tag="PL000_10895"
     CDS             complement(5100..6545)
                     /locus_tag="PL000_10895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC89452.1"
                     /translation="MAQPNYQDYLTILPLITLIVWSCVLLLVDLFIPKKQKGWTAFLA
                     AFGLVVTLGLTIGQIGQSNTGFSGMVLVDGFSTFANVLLLTSGLFGIALAFGYTRRMG
                     IERGEYYTLLIFSVSGMMLMAQAADLIVVFLALELLSIPLYVLAAFSRANSQSQEAGV
                     KYFLLGAFATGFVVYGTALVFGATGTTSFSGIFYAVSNGASILLLTIGAALILVGFGF
                     KVAAVPFHMWTPDVYEGAPTAVTAFMSSGAKIAGFAAMLRVFSSAFPALSADISDILW
                     AFAALTMVVGNLTALSQTNIKRLLAYSSIAHAGYILMAFVPYGNSEVSAVSIAAGLFY
                     LVTYALTNFGAWAVVITLEKSEGKGLEINDYAGLAKKYPALAAAMTVFMLSFIGFPPT
                     LGMVGKFYLFRAAVAGGYNGLAIIGVVTSLVSAYYYLRVVVTMYMREGDPTIEREPLL
                     SITTAVTAIATVVLSFVPQFLFLLSSTALVR"
     gene            complement(6633..8144)
                     /locus_tag="PL000_10900"
     CDS             complement(6633..8144)
                     /locus_tag="PL000_10900"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC89453.1"
                     /translation="MEFLLQPLTLLTFLPLVGVLVILFMNSESKNAIRWVALGTSLIT
                     FVVSLWVFSQFDSSNPDLQLEAKYPWIQVAGWNIYYYLAVDGLSILLVLLTAFLTPIS
                     LLSTWTAVEERVKDFMVFFLLLEVGMMGVFLAQDLFLFYIFWEFTLVPMYFLIGLWGG
                     PRRVYAAVKFFLYTMAGSILMLIAILFLGIKTDTFNVPTMLARLPDLYATSINPQTQM
                     LLFIAFAAAFAIKVPMWPLHSWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFNIALF
                     PDATVAAAPWIALLATIGIIYGAAVSYAQQDVKKLVAYSSVSHLGFVMLGLFALNAQG
                     VAGAILQMINHGLSTGALFLLIGMIYEQTHTREIKVYGGLWKITPVFGTLMLIASLSS
                     MGLPGLNGFVGEFTILLGAFGSKAIANPWYAGVSAIGVIMAAVYILYMFQKVFLGPAG
                     EITHHHELKDLNWREILTVAPLVIFMFWIGLYPKPFFDILAPAVEKLLSALPL"
     gene            complement(8168..10357)
                     /locus_tag="PL000_10905"
     CDS             complement(8168..10357)
                     /locus_tag="PL000_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC89454.1"
                     /translation="MHLSESVSTGFFFLAPWLVFAPVIGLLINLIFGKQFSEKMVGTV
                     ASLASGTAFVVSVLLAYSVSLNHGEVVRWKLAEWIHVGTLHLDWTFRMDSLSTTMMLV
                     VSGVGTLIHIYAIGYMHEDVRFKHEEGRFNRFFLYLNLFIAAMMILVSGDSYMMLFVG
                     WEGVGLCSFLLIGFWFEMDTLARPSWANSNAAKKAFITNRVGDFGFLIAGFLMFWYLG
                     SFQFDEAFKAAPEIAHSMPWVIVAITLFMLVGVAGKSAQIPLYIWLPDAMAGPTPVSA
                     LIHAATMVTAGVYLVTRSAPLYTLVPQAQYIVAMVGAGTALFAATIAVGQYDIKKVLA
                     YSTISQLGFMVAAVGMGAYVAGMFHLITHAFFKALLFLSAGSVILGLERGHHAHAHHA
                     AHDDHGKKKKEGKHASGKAEGKKEEEHGHGGHDEHGEVFDPGDMRNMGGLRKTMPVTF
                     WLYMIGTLALAGIFPFAGFWSKDEILLDASLHFTSVYWQLTIAAFFTAFYMGRQIWMV
                     FFGEARTDAAKHAEESPKVMTVPLMVLAVLSVAGGALNLPFEGFHNLGHWLGHTIGEV
                     EGLPLDLKVAGISTALALLAILISWFIYGRNPLKAGQIDPLKKPLGFIFTGMEGKWFV
                     DEGYFAVIINPFNKLSQFLADVIDWRFWHDFVHDTVIAGTYNWLSETALNHYADQKGI
                     DGFANWLGTATQSVSATLRKVQNGFVRSYALSVLLGVVLILGYLILK"
     gene            complement(10419..10739)
                     /gene="nuoK"
                     /locus_tag="PL000_10910"
     CDS             complement(10419..10739)
                     /gene="nuoK"
                     /locus_tag="PL000_10910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC89455.1"
                     /translation="MVPVNYYVALSAVLFTIGALGVLIRRNPLIIFMSVELMLNAGNL
                     AFVAFGRSYEIAGGQPFFSGQIFVFFVIAVAAAEVAVGLALIVEIFKARKNINIDELN
                     SLKG"
     gene            complement(10739..11263)
                     /locus_tag="PL000_10915"
     CDS             complement(10739..11263)
                     /locus_tag="PL000_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HNC89456.1"
                     /translation="MNSDIIVFLVLSLIAISSALGMLFSRNAVYSALYLVLNFVTVAV
                     FYLLLGAPFIAMSQITVYAGAIMVLFLFVIMLLGAENLAPSQSLPWQKPLAYTLAVVL
                     VVESVYLVISRAQTDKFISTPDAALNSMENLREMAMSLFNQYLLPFEITSILLLVAMV
                     GAIVLTKQEKREQK"
     gene            complement(11334..11855)
                     /gene="nuoI"
                     /locus_tag="PL000_10920"
     CDS             complement(11334..11855)
                     /gene="nuoI"
                     /locus_tag="PL000_10920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HNC89457.1"
                     /translation="MGVFDPIIELSKGLGETLRSFLFEKTVTYQYPEEKRPVRPRYRG
                     RHVLKRYDNGLEKCIGCSLCAAACPADAIFVEAAENTDEKRFSPGQRYASTYEINMLR
                     CIYCGFCEDACPTEAIVLGDNYELSFTDRRQAIYPKEMLLEPVRTVDMITPRKVEPGV
                     FTRSVPEMQNPSD"
     gene            complement(11842..12981)
                     /gene="nuoH"
                     /locus_tag="PL000_10925"
     CDS             complement(11842..12981)
                     /gene="nuoH"
                     /locus_tag="PL000_10925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNC89458.1"
                     /translation="MDWTIWLEWVLKGFILCLILLTGFAYLTLYERRALARMQVRVGP
                     NRAGPQGVLQPIADAVKLIFKEEFTPSRVYKVVFVLAPVLTVVPSLILAAVIPLGSKF
                     VLFGREITLYVADLNVGVLYLTSIASIAVYGIVLAGWSSNNKYAMLGGVRSSAQMISY
                     ELSLGLCIVIAILLGDSMNLNDIVNAQKGMWFAVIQPAGALVFMIVTLAEVNRAPFDM
                     PEAEQELTAGYHAEYSGMKFALFFMAEYQKMIVICMIAATLFFGGYREFWFLQGTIFS
                     VDRFWFLGPIYLLLKVIVLLFFMIWIRATWPRIRYDRLMSFGWKVLLPLSLILAFITA
                     TGILLAQELNNPMIIWGIPVLSIVCVLVAVVFINSDLRRKSHGRI"
     gene            complement(12983..15451)
                     /gene="nuoG"
                     /locus_tag="PL000_10930"
     CDS             complement(12983..15451)
                     /gene="nuoG"
                     /locus_tag="PL000_10930"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HNC89459.1"
                     /translation="MTKQVTLSINGITVTVPDGTLIADAAKMAGIDIPVFCHHPKLEP
                     AGMCRMCLVEVGRPMLDRATGQVVMENGQPKIQFMPKLETACTNRVSDGMVVMTTTDK
                     AKDGQKGTVEFLLTSHPLDCPICDKGGECPLQNLTMAFGPGKSRFIYDEKAHFEKQVP
                     LGDLIWLDRERCIQCARCIRFQDEVAGEAVLGFDERGRNTQIISLSEPGFDSIFSGNT
                     TDICPVGALTTADFRFGARPWELKAQASICTQCPVGCNTTLNTRREAKAGGDVVVKRV
                     MPRQNEEVNEIWLCDKGRFTYHFTESKKRLTRPLVRKEEKLARASWDAATKMAAENFT
                     KSKKDFVVLASGRLANEDLFNLKSLADTAGGTAILYSNMGGGDLTTLVGVGQGTNIGK
                     MGKGDAILVVASDLYQEAPIWWLRVKQAADRGATLIVANARETKLDKFAKFVIRYAQG
                     DESKAVANLSNKGKIADEFAKAKNAVVFYGNDGLGLNDTASLASACATLLKDTEHVGK
                     PNNGLVAVWERVNDQGAWEIGFRPEPDLEKALKGKAVYIVGADPVGDDPALAKALKGA
                     KFVAVQDILETATTEIADVVFPAQAYTEREGTFTSGERRVQRFYAAVPVKGDAKPDFA
                     ITSQIAKQMGVILEGSSASVVFDILSHSVESFADLSYAKLAETHGQWPIVGRGDLYYG
                     GTTYENTQGLGVQLVPTAQAGGTVRIPKVRKEAALRPKENELLAVPVTRLYDYGVTIS
                     SSAPLLGQWVGEPTVTLHPMAAKNLGVEAGTRVKLSFDGVNAEVVVKLDDTISAGVAL
                     VPRSMGIAIREPVVARVQLAGKVK"
     gene            complement(15462..16709)
                     /gene="nuoF"
                     /locus_tag="PL000_10935"
     CDS             complement(15462..16709)
                     /gene="nuoF"
                     /locus_tag="PL000_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC89460.1"
                     /translation="MANVLLRHRDIADLNKIEVYKKNGGFDAFKKAVSKMQPNEVTDV
                     VKNSGLRGRGGAGFPTGMKWSFIDTKNWPHYVVANADESEPGTFKDREIMESNPFQFL
                     EGLAIASYAVGANVAYIYLRGEFWQVAAFLDEKIADMEKAGFLGENLFGTEYSLKIYT
                     HLGAGAYICGEETALLESLEGKRGQPRVRPPFPPSFGLYGKPTIINNVETFTNVPLIM
                     ANGAEWYKSMGTADSAGVKIFSLSGRVRKPGNYELPFGTTFRQLIYEHGGGVLDGRPV
                     KAIMPAGASSSLIVVDDKVLDTPMDYATVRTLGADLGSASVIVLDDTVDIDWVVNKTI
                     HFFKHESCGKCTTCREGNYWMQHLTERIHSGQGVQNDVDLLLNVAKQMQGKCLCALGE
                     FSTMAVVTGIERFPQDFKKAVKA"
     gene            complement(16709..17215)
                     /locus_tag="PL000_10940"
     CDS             complement(16709..17215)
                     /locus_tag="PL000_10940"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC89461.1"
                     /translation="MSLEKSYPKEVKQILSKYPPEHKRSAVMPLLYLAQREEGYVNKA
                     AMQDIARMLDITETEVASIVGFYSLYHDEKAGKYRMQICTDLPCALRGADDFMKNLCS
                     NLGIKVGETTPDGLVTLEAVTCLAACDKAPMFQTQGPDGIKYHENMTVDKTIELIEAL
                     KKSDGGVK"
     gene            complement(17212..18441)
                     /gene="nuoD"
                     /locus_tag="PL000_10945"
     CDS             complement(17212..18441)
                     /gene="nuoD"
                     /locus_tag="PL000_10945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HNC89462.1"
                     /translation="MSQIEEVSLEKFKHLVSERALTGETMLLNMGPQHPSTHGVLRLL
                     LELDGEIVVNCIPDIGFLHTGVEKNMEAKTYQKAEVMTDRLDYMNTMGNNLAYCMAVE
                     KLVDLDVPVRAQALRVILVELQRIASHLVWLGTSGLDLAAMSMFLYCFREREQILDIF
                     ELVSGQRMMTTYFRPGGVWRDVPVEFEKAVRDFIKIFPKRIAEYESLLTKNPLFIDRM
                     VDIGYLSKETALSYGVTGPTLRASGVDWDLRKVRPYCGYEQYDFNVPTRTEGDTYARY
                     LVRIEELHESLKIVEQALNKLPLGPVRSENRKFVPPPRSEIGVSMEALIHHFKLWTEG
                     FSAPQASIYNAVESPRGELGLFIEGDGGPKPLRMHLRTPSFDNLAVVPQIVKGHLVAD
                     LVAILASIDIVLGDIDR"
     gene            complement(18444..18956)
                     /locus_tag="PL000_10950"
     CDS             complement(18444..18956)
                     /locus_tag="PL000_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC89463.1"
                     /translation="MNKNLEPVVKTIQDKFGAGCEEFRDEVHLFVKPEQIVEALTLLR
                     DEQGFELLSAYTAVDYYPSETPRFHLIYQLTSLVKNLSVQVRVAVNGSSPKVPTVTGV
                     YASANWREREIFDMFGIEFEGHPDPRRILMPEDHVGHPLRKDFPLGYEEPQFTFNFDE
                     IDLRKPYVKE"
     gene            complement(19011..19499)
                     /locus_tag="PL000_10955"
     CDS             complement(19011..19499)
                     /locus_tag="PL000_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNC89464.1"
                     /translation="MGVEQKLGNMGVVTTTLENVVNWSRTNAMWPMLFGLACCAIEMM
                     AAQASDYDMSRFGMELMRASPRQSDLMIVAGRVSKKMGPVLRRLYDQMPEPKWVIAMG
                     DCASCGGVFNNYAIYQGVDEVVPVDVYVAGCPPRPEALIHGVLTVYEKVKGEKFKDLA
                     AK"
     gene            complement(19490..19846)
                     /locus_tag="PL000_10960"
     CDS             complement(19490..19846)
                     /locus_tag="PL000_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC89465.1"
                     /translation="MLLEYIAIGVMIAVATLIAFIAIGLGELFGPKKKTAAKDMPYES
                     GMNPYGEGTRRMPVRFYLIAVLFILFDIEVVFFLPWAIAFRKLGIFGLLEMIVFIVIL
                     LIGYVYAWKKGALEWE"
     gene            complement(20057..20905)
                     /locus_tag="PL000_10965"
                     /pseudo
     CDS             complement(20057..20905)
                     /locus_tag="PL000_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(21335..21583)
                     /locus_tag="PL000_10970"
                     /pseudo
     CDS             complement(21335..21583)
                     /locus_tag="PL000_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            complement(21711..23615)
                     /gene="dnaG"
                     /locus_tag="PL000_10975"
     CDS             complement(21711..23615)
                     /gene="dnaG"
                     /locus_tag="PL000_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNC89466.1"
                     /translation="MSTIDEIKARIDIVDLVSEAGVKLRHAGKNYTGFCPFHDNKHTP
                     AFVVWPESGTWRCFGQCNEGGDIFKFVMKRDGLDFKEAMQKLALRAGVEIKEYQRETP
                     QQKEAYDHLRKLLEDAVVYYRTQLFGAPAILAYLHEKRGLTDSTIETFGLGYAPPGYD
                     NLLKHFLSKGYSQQDLIDSGMVTVRENDEARTYDKFRNRIMIPIRDENGKMAGFGARI
                     VDPNDVPKFLNSPETPIFSKGRLLYGLDRARKPIRAADQAVIVEGYLDVIALHQAGYE
                     NVVSPMGTALTEDQLRLLKRSTRRIVLALDPDAAGQKAVLRGLDAARSAMDREGELGF
                     DARGLLKNEARLQADLRVATMPDDLDPDEIVARDQEEWKKLIENAKPIVTHVMDSLAA
                     GQNLNDAKVKNQIAAQVLPLIEDLPSAMERDTYRQALARMLRVNESVLTGTQVQGPVA
                     RRKPRSVEQSHKVVSSGIVAVNPTLKIESYCLSVLLRKPELLYRLDKRMQEADLAALA
                     VEDFEYTDHQLLFGVVRMAVEQDEKDYHHYVMAHVPESVSELSKELVARSDALDPVED
                     KILDDLLARFMDLRRANAMLNVNQLRFLQEEDQQQGGANMKLYQEQTLQMTRLLHSLD
                     QAKRKLSSKR"
     gene            23645..25246
                     /locus_tag="PL000_10980"
     CDS             23645..25246
                     /locus_tag="PL000_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89467.1"
                     /translation="MNSRLIKNKVFALPFLAVYAWLVIRTSWVGDDAIITFRSLENFL
                     HGYGPVFNIGERVQTFTHPLWFLLQAAAGAVFNLWKSNPFGQGQMYFLNVFISIGISL
                     LAAAIFTFNETSSAKGAALGLLIFSVSKSFVDYSTSGLENPLTHLILLLFTLTYVGPA
                     ENSQRRLYLLATLAALGGVDRLDTLLFYFPALVLLFWESPHKGKTFLTLGFGFLPLIA
                     WELFSLFYYGVPFPNTAYAKINTGIPEASLFMQGLFYYLNSLKLDPITLLTIFTVSAA
                     GWMSKNKKHQAILAGVLLYLLYTIYIGGDFMSGRYFSSPLLVCVTLLATMDFESPKKF
                     GVAVGIVLLVGLAPIAVVAERAPAFGVGDEGDHRVFVDEHGISDERIVYTGLGLFERL
                     KKKSAPSTNYARDKWVYLPDEPVRVRIVGPLGLNSMTLGPNFHVIDLNSLADPLMTRM
                     PLYEIDRWRIGHFRHIIPKGYEETLKSGENRIVNKDIAQYYDKLSFIVRGELWSWERV
                     VEIWNFNTGKYDYLLENVTPADAME"
     assembly_gap    25381..25480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25481..27029)
                     /locus_tag="PL000_10985"
     CDS             complement(<25481..27029)
                     /locus_tag="PL000_10985"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNC89468.1"
                     /translation="MADQNNGLEYIEQLLRDGKKQEGRTALVQYVQSHPDSSRAWWML
                     SFILTDPKQQLDCVERVLKLDPGNIPARNRLLKLQSDLAPKSDSTFSQEPSRLHPITA
                     PFILDDESAPPTSSDAFEASFLRQSQGNVRPPEAATFLPDDSIDDRDAFNISNSSYQA
                     AQDNYKPSPFFPEDDLDLQSAPPLSAFERSYSNTPSQDPYQSSSIPPLFLPEDEDLPR
                     PSADAFEHSFGQDAYENPYSSSVSPFQTETQSADKPAQPARKKPAPKKKNDFVKYIVL
                     IVVFLCVFGLILVGFFFVLRGQQISTPTAQPIFLGPTETPTTIPTIGLPPTWTPAPSP
                     TLIPTSTLGYIESTSTPISNVIGTEDPNAETQVGLGTGMAAPDFTLKNVNTGNQVSLS
                     DYKGKPVVIFFWANGCGYCDTEAAAMQDIYTQYKDIGLVVLAVDVWGDSDHARTYRDN
                     HNLTYPMLIDSKASTFKKYGGTNLFPVNYFVDVNGNISFGQVGMMDYSMLNMNVRRLL
                     NLIPTVSP"
     gene            complement(27200..>28204)
                     /gene="glyS"
                     /locus_tag="PL000_10990"
     CDS             complement(27200..>28204)
                     /gene="glyS"
                     /locus_tag="PL000_10990"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNC89469.1"
                     /translation="LTFHTKLGSMLDKSERIVKLVNELIPSLGLEADQAIFARRAAHL
                     MKADLVTQMVTEMTSLQGIIGGEYALRSGEQKEVAAAISEQYQSVPQTKAGLTIALAD
                     RLDSLVGMFGAGLAPTGAKDPFGLRRAAIGVVMPLMSPAVGMSPMDFDLAKAVKLSAQ
                     TQPIPVSDEVQKQILEFITGRMKVALGDMGFKHDVIEAVLAAQSNNPAGTVRAVDQLS
                     AWVEREDWSSILDGFARCVRITRDQKETFKVSEKSLVEEAEKGLYEAVNRLPPVVAGD
                     VDSFLNNVSALIPAITAFFDKVLVMVEDKAVKENRLGLLQKIAGLSNGIADLSKLEGF
                     "
BASE COUNT         7127 a         7318 c         7227 g         6432 t
ORIGIN      
        1 acatacgcat cgctgatctg ggcttccatc tggtcgccgt tggtgatgaa gtagggggag
       61 agataaccgc ggtcgaactg catgccttcg acgaattcag tttcgaactg catggtcttg
      121 gattcttcaa cggtgatgac gccgtcttta ccgaccttgt ccatcacatc agcaatgagt
      181 tcaccaactt ccacatcagc cgcagagttg gtcgccacag aagcgatttc ttcgcgggtg
      241 tcgatcttca cggagttctt cttgagttca gtaacaatgg tctctgtggc gagctcaatg
      301 cccttcttga ggagcatcgg gttgtagcca gcttcgagag ccttgaggcc ttcgttcacg
      361 atggcgtgcg ccaaaacggt ggaggtggtg gtaccgtcac cggcaatgtc attggtcttc
      421 tgagcggctt ccttgaggag ctgggcgccc atgttctcga aaggatcttc gagttcgatt
      481 tccttcgcaa cggaaacacc gtcgtgagtg atggtggggg agccgaactt gcggtcaatt
      541 gccacattgc ggcctttggg gccgagggta gcagaaacag cttctgcaac aacgttcatg
      601 ccgttcttaa gcttgcggcg ggcgtcttct gaaaatacga tttgtttagc agccataatt
      661 cacttccttt gaatttctgt ttggtggatt aaccgacgat accgagaata tcgctctcgc
      721 gcagaatgag gaccttcttg ccctcgatct tcacttccgt gccggagtac ttggcgaaaa
      781 gcaccacatc gccaaccttc acatccatgg cgatgcgctc gcccttgtca ttgcggtcgc
      841 cagggccagc cgccaaaatc ttgccctgct ggggcttttc cttcgcggtt tcgggaagaa
      901 cgataccgcc agcggtcact tcttcctgtt cgatgggctc aaccaaaacg cggccaccca
      961 ggggtttaaa tgagagtttt gccatttctt gcctccatta agagatttga atttttttga
     1021 ataagcacag aattagcact caatacttgc gagtgctaat tgcaatatta cctacttaaa
     1081 ttggtcaagt caaggggatt gtaaaaattc ttacaaaact ctgatggatg aaaagcccgc
     1141 ctctcccctc actgataact gaatctgttt caaacggcat tctcgccccg ctaaattggt
     1201 tacggggtcg cagtgggcgt cacatccaat gtttctggcg tagccgcagg ggactccccc
     1261 gccgcgggag catcattcag cgtggaacag atcgtccgca ccacaacgat atccgcggca
     1321 atgttcgggt cattatcaaa ttccgaagct tcgatttcat cggcaatatc caatgcgtca
     1381 ccgcaatagc tttttgaagg cgtactcaaa gccgccaatt cagaggcata aatatcataa
     1441 taatagaccg tattcgaagt aagcggcaat ccgctcacct cagcaggaac atcaccacct
     1501 ccgcaacctc cgcgcccttc acaggattca tcggggttgc atccgcgaat ggcgcatttc
     1561 agggcaagga tacccgtttc ataattacgg ttcttgtgat acagcacccc caactcgcca
     1621 taaaactcgg ccttatcagg ctggaattca atggtctttt gcacattgcg gatggcaata
     1681 aaaaactgac ccatctgcga ataggttttt gcgatcgaaa gatatggtgc agggtcgttg
     1741 atccccaact gatcgttgat ggtggcagcc cgatcaaagt actccagtga tttttcaaaa
     1801 agctgggtct gtacttcctc gtttgggctg ttaaaggcaa ggatacgata attggcccct
     1861 gcccgaatgt gcaggaaagt taaattggga gcgatcaata ttgcacggtc ataggcctca
     1921 atggcaagcg catactctcc cagcgattca agcacatacg catggactcg atgcacatcc
     1981 atcaatgatg ggtcacgctc caacgcctgg tcaatgattt gctgggcatt ggtccacttc
     2041 tgctggtcca ccaaaatttc ggcgtagaat gccaaagcgc gtgtgttgtt gggatccaat
     2101 tgcaaggcac ggacggcggc gccattggct ttagtcaaaa gacgttgcac ttccctttca
     2161 tcaggcacaa gcaccgggtt ggcgttccag tccagcacaa aggcataaac agactgggcg
     2221 taactgtcat cgggtgaaat ggcaactgcc ttctccgccg atgcaagcgc ctcagccaga
     2281 cgcaccttgg tatcggcatc gctcaccatc atggctgaag aataggtttg gatgcgtgcc
     2341 aaatcggcca gcaatttggc attggagggg tctacttttg cggcttcacc gtaggctgtg
     2401 atggcggcat ccaattcacc tgcagtaaat tgagcatccc cctcaagcgc ataggacagt
     2461 gcaaaacgcg ttggggttgg tgtgggataa aacaaaggtt ttacatcgcc gctctgcact
     2521 gaacggatca accagattcc gccaaggatc aggatggtcc aaaagaacat gcggtagata
     2581 ttgatctccc cccgcttccg aaagatgtca cggcgttggt aaatgttcat ggtgtcgggg
     2641 tgggggtttc tgttgaaaca ggagcagttt caggcgaagt gggaagcggc tccggcgtct
     2701 gtaccagatt ctgttcgcac ttactgatga tcgcgttggc attttccact gatagttcat
     2761 cagcaggaat gcgttccaga attgtgcggg caatattcaa ggcttcacca catttgttgg
     2821 tgcgggcaag cgcgaggcca tatgtaaaat agtattctgc aagacgtatg ttgtttggca
     2881 caagcggcat cgcctcgatc tgaactccgt cttttgtaaa gccgccatcg accacataac
     2941 ccaattccac aagcgcttcc gtccagtagc catttcgata atacattacg ccaaggttac
     3001 cgcgcaggct ggggtcggca ccatcattgg caacagcctg ttccgcatat tgcatggctt
     3061 ttccaaattc accgcgggtg gcataagtgc gtgaagtgag aaaatcgggg gtggggtctt
     3121 cagggttcaa agcatcggcg tttgaaaatg cggtaattgc atcttcgtaa ctgccaagtg
     3181 cgcggtaatt aattcccagt gaaagataca ggtcggcgat cttcgggttg aggtcaatgg
     3241 cttgctgata ttcgcggatg gcattttcgt aatcgcccac agcctcaaga ataattccgc
     3301 gtgcgcggtg ggtttcgagg gtattgggtg ccaaagccac ggcaattcgt gattcgtcaa
     3361 ttgctttttc aatgacatca aaggaacccg aatccaccag gatttcgaca taatatgcgt
     3421 gcgccaatgc attgttgggg tcaagggtca gggcacgttc aatgctgtct tccgcttcga
     3481 taaacttttc ctgggcaccc aaagcccagg ccagcacagc atgcgccatg gaatttgcat
     3541 tgttaagcag aatcgcactt tccgcgcttt cctgtgcggc ttcgtactgg ccagcaaaca
     3601 cctgcgcctg cgccagtgca atatacaccg tggggtcatc aggacgggaa aggatggctt
     3661 gtttgtaaga atcaatcgcc cgcagtaatt gtccctgctt gaaatattcc tgtgcctctg
     3721 tgacaaatga ttcaggagaa cgtgtgactg tgggggtggg caaaaacggc gatggccgtg
     3781 cggcaatgac aaatcgattc acgtaggacg cgcccagcac caacaaacac aacaaaatta
     3841 tgcggaacca gtttggacgg gtccgcctgc gattcatgct ccacttgctg ccttttagat
     3901 acatcgtttt ttatattacc attttcatga aatcaacgtc aagcaggggg tacgttctct
     3961 catttgaatc taagcggatt tatggactgg atgccccgaa aaccccatct ttttgaagac
     4021 acaatcgtta cacgaagtca gcgtttcgac cttaactcaa aaatggttta aacagtccaa
     4081 acacaacctc ccccgtaaac attctcagaa agataaaaga aaaaggtctg actttatgaa
     4141 atcagaccta atccatagtg actccgttgg gatttgaacc caaaaccaat tgattaagag
     4201 tcaactgctc tgccgttgag ctacggagcc gctctcaaaa cttgcgagcg gtattatacc
     4261 gcaaagaaaa ggggtgtcaa taaaaaagtt gactagcggt atggctttct caaagtcggg
     4321 agtacttgcc caacatagat aaaactaacc gctaagagcg caaagagcgc gaagtttaat
     4381 ggattttctt ggcgttcttc gcgggcttgg cggttcgaaa ggcttgtcag atattaattt
     4441 gagaaagcca taagactacc gtttgcatgg ggcactccat acgttgaccg gtctgaaacg
     4501 cttcacctga ccttcatccc atttcattga agtgaaaaac aacctacaat ctttttatct
     4561 aaggcctgcg gtataagtca ttcccctatt aagaaggtgt gatatgtcaa atagctctaa
     4621 tggcgtctgg aaaacctttt ttggctgtgg cggattctcc ttgtttttgc ttacagtgat
     4681 gttgttagcg gttgttttag cttgcagcgc aacggtggcg gtccccattt ttgaccggga
     4741 tgtcctgcaa gaaatcgcat gtccaccagg cagcaccctc attaccggtt gggaggaaac
     4801 cacctacacc cgccccggcg aaagggtcct ttacgggtat tgtgtggacg cacaaggtaa
     4861 cgaatcaccc gcacaagatc taggctacgg cgcattggaa tatttcccca ggtattttgg
     4921 catctctctc gcagcaagtt tggtattgac ccttcttgtg gtcattccac tcatgatcct
     4981 gtatcgggtt atcaagaaga aattcttctc aaaacccgtt cccactgacc ctgctattgg
     5041 gagtttataa taaaaaaagc gatgacttga aggtcatcgc ttttttgttg aaatcaagcc
     5101 tatcttacca gcgccgtact tgacaacagg aacaagaatt gagggacaaa gctcaaaacg
     5161 acagttgcaa tggctgttac agcagttgtg atgctgagca aaggctcgcg ctcaatggtc
     5221 gggtcacctt cacgcatgta catggtgacc accacacgca ggtagtaata cgctgaaaca
     5281 agcgatgtca ccacaccgat gatggcaagg ccgttgtatc cgccagcaac tgcggcgcgg
     5341 aacaggtaga attttccgac catgccaagg gtgggcggga aaccgatgaa ggagagcatg
     5401 aacacagtca tggcggcagc aagggcggga tatttctttg caagaccggc ataatcattg
     5461 atctcgagcc ccttgccttc cgatttttca agggtaatga ccacagccca tgcaccgaaa
     5521 ttggtcaatg cataggtgac cagataaaac aggccagccg ctattgaaac agcagagacc
     5581 tcggaattcc cgtaaggcac aaaagccata aggatgtacc cggcatgagc aatacttgaa
     5641 tatgccagca ggcgcttaat gttggtttgg gataaagcgg tcagattacc aaccaccatg
     5701 gtcaacgcgg cgaacgccca aagaatatcc gagatatcgg cggaaagggc gggaaaagca
     5761 gaggagaaaa cgcgcaacat ggcggcaaac cctgcaatct ttgcgcctga agacatgaac
     5821 gctgtcacag cagtgggggc accttcatat acatccggag tccacatatg gaagggaaca
     5881 gcagcaacct tgaagccgaa accgacaagg atgagggcag ccccaatggt cagaagcagg
     5941 atcgaggctc cgtttgaaac ggcgtaaaag attccgctaa aggatgtagt ccctgtcgct
     6001 ccaaaaacga gagccgttcc gtagacaaca aatcctgttg caaatgctcc caacagaaaa
     6061 tatttaactc ccgcctcctg agattgggaa ttggcccgtg aaaaggctgc cagcacatac
     6121 aacggaatgg acaaaagttc aagcgcaaga aaaacgacaa tcaggtcggc ggcctgcgcc
     6181 atcagcatca tgcctgagac gctaaaaata agcagggtgt aatattcacc gcgctcaata
     6241 cccatacgac gggtgtatcc gaaggccagt gcaattccaa atagtccgct ggtgagcagc
     6301 aacacattgg caaaggttga aaagccatcc accaacacca tgccgctaaa gcctgtattg
     6361 ctttgcccga tctgaccgat ggtaaggcca agtgtaacaa caaggccaaa ggcggcaaga
     6421 aatgccgtcc aacccttctg ctttttgggg atgaacagat ccaccagcaa aagcacacat
     6481 gaccaaacga tcagtgtgat cagagggaga atagtcaagt aatcctgata attaggttgc
     6541 gccatgaacg ctccaagaaa atgcaggttg cagaccgcag gttgaaagtt acaggtatac
     6601 acctttggct ttcaacctgc caaccgtcaa ccctataaag gcaatgcaga taaaagtttc
     6661 tcaaccgcag gggcgagaat gtcgaagaat ggtttggggt aaagaccgat ccagaacatg
     6721 aaaatgacca acggagccac ggtgagaatc tcgcgccaat tgaggtcttt gagttcgtgg
     6781 tggtgggtaa tctcgccagc agggccgagg aatacttttt ggaacatgta cagaatatac
     6841 acagccgcca tgatgacacc gatggctgaa acacccgcat accacggatt ggcaatggcc
     6901 tttgaaccaa acgcgccaag caaaatggtg aactcaccca cgaagccgtt caaaccaggc
     6961 aggcccatcg aagaaagcga agcgatcagc atgagtgtgc caaagacagg ggtgatcttc
     7021 cacaaaccgc cgtagacttt gatctcgcgg gtatgggtct gttcgtagat cataccgatg
     7081 aggaggaaca gcgcgcctgt gcttaacccg tggttgatca tttgcaaaat ggcacccgcc
     7141 acaccttggg cattcaacgc gaacaagcca agcatcacaa agccgaggtg gctcacggat
     7201 gaataggcga ccaatttctt aacatcctgt tgggcatagg aaacagctgc gccgtaaatg
     7261 atgccaatgg tcgcaagcaa cgcaatccac ggggcggcag cgactgtcgc atccgggaag
     7321 agcgcaatat tgaagcgcag gaaaccatag gtacccatct tcagcaaaac gccagccagg
     7381 atgacagaac cagcggtggg cgcttccacg tgggcatcgg gcagccacga atgcagcggc
     7441 cacattggca ctttgatggc aaaagccgca gcaaaggcaa tgaagagcag catttgagtc
     7501 tgcgggttga tgctggtggc atacaggtca ggcagacggg caagcatggt tgggacgttg
     7561 aaggtgtcgg tcttgatgcc gaggaacaaa atggcgatca gcatcaaaat ggaaccagcc
     7621 atggtataga ggaagaactt gaccgcagca tatacgcggc gaggtccgcc ccacagaccg
     7681 atgaggaagt acatcgggac gagggtaaat tcccagaaga tgtagaacag gaacagatcc
     7741 tgggcaagga aaacgcccat catgcccact tccaaaagca ggaagaagac catgaaatcc
     7801 ttgacccgtt cttcaaccgc tgtccacgtg gagagcaggg aaatgggggt caggaaggcg
     7861 gtgagcaaca ccagcaaaat gctcaagccg tccacagcca gatagtagta gatgttccag
     7921 cccgctactt gaatccacgg gtatttggct tccaactgca gatcggggtt ggacgagtcg
     7981 aactgggaga aaacccacaa cgaaacgaca aaggtgatca atgatgtgcc caacgcaacc
     8041 cagcgaatgg cattcttcga ctcggaattc ataaagagaa tcacgagcac gcccacaagc
     8101 ggaaggaagg tcaggagtgt aagaggttgc aaaagaaatt ccatgttcta tcctcggttt
     8161 ctacgagtta tttcaaaatc aaatagccta agatcaaaac cacgcccagc aaaacagaga
     8221 gcgcatacga acgcacaaag ccattttgca ctttacgtaa cgttgcggaa acggattgtg
     8281 tggcagtgcc caaccagttc gcgaaaccgt caatgccttt ttgatcggcg tagtgattca
     8341 gtgcggtttc gctgagccag ttataggtcc cggcgatgac cgtgtcgtgg acaaagtcat
     8401 gccagaagcg ccagtcaatc acatccgcaa ggaactgtga gagtttgttg aatggattga
     8461 tgatgaccgc gaagtagcct tcatccacaa accatttccc ttccatgcct gtgaagataa
     8521 aaccaagcgg cttcttgagc gggtcgatct ggccagcctt aagaggatta cggccataaa
     8581 tgaaccagga gatcaaaatg gccagcaaag ccagtgcagt ggaaatgcct gcgactttca
     8641 ggtcgagcgg gagtccttca acctcgccaa tggtgtggcc cagccagtga ccgagattgt
     8701 ggaacccttc aaacggaaga ttcaacgcgc cgcctgcgac cgataaaact gccagcacca
     8761 taaggggcac ggtcatcact ttggggcttt cctctgcatg tttggctgca tcggtgcgcg
     8821 cttcaccaaa gaagaccatc caaatctggc ggcccatgta aaaagcggtg aagaaggcgg
     8881 caatggtcaa ctgccaatag acactggtaa agtgcaggct tgcatccaaa aggatttcgt
     8941 ccttcgacca aaaaccagca aacgggaaga tacccgccaa ggccagtgta ccgatcatgt
     9001 acagccagaa tgtgacaggc atggtcttgc gaagtccgcc catgttacgc atatcacccg
     9061 ggtcgaagac ttcaccgtgc tcgtcatgac cgccatgccc gtgttcttcc tctttcttcc
     9121 cttcggcttt gccagaggcg tgctttcctt ctttcttctt tttgccgtga tcatcgtgag
     9181 cggcatgatg agcgtgggca tgatgtccgc gctccaaccc aagaataacg gaacctgcag
     9241 aaaggaagag cagggccttg aagaaagcat gggtgatgag gtggaacatc cctgccacat
     9301 acgcgcccat gcctacggca gccaccatga aaccaagttg cgaaatggtg gaatacgcga
     9361 gaactttttt aatatcgtat tgaccgactg caatcgtcgc ggcgaagagc gcggtccctg
     9421 cgccgaccat cgcaacgata tactgcgcct gcgggacaag agtgtaaagt ggagcggaac
     9481 gcgtcacaag atacacacca gcggtcacca ttgtggcggc atggatcaac gccgaaacag
     9541 gggttggacc cgccatcgca tctggcagcc agatgtagag cggaatttgt gcagatttac
     9601 ctgcaacacc caccagcatg aacagggtaa tcgctacgat cacccaaggc attgagtgtg
     9661 caatttcagg ggcggccttg aaagcctcat cgaattgaaa agagccaaga taccaaaaca
     9721 tcaggaagcc agcgatgaga aaaccaaaat cgccgacgcg gttggtaatg aaggccttct
     9781 tggcagcatt ggaattggcc caggaaggac gtgcaagtgt gtccatttcg aaccagaagc
     9841 cgatcagcag gaaggaacaa agacccacac cttcccaacc aacgaaaagc atcatgtacg
     9901 agtcgccaga gacgaggatc atcatcgccg cgatgaacag gttgaggtaa aggaagaagc
     9961 ggttgaacct tccctcttca tgtttgaagc ggacatcctc gtgcatgtat ccaatcgcat
    10021 agatgtggat gagggtccca acgcccgaaa ccaccagcat catcgtggtg gagagcgagt
    10081 ccatgcggaa ggtccaatca aggtggagtg tgccgacgtg gatccattct gccagtttcc
    10141 agcgcaccac ctccccgtgg ttgagggaaa cagaatatgc cagcagaacg gaaacgacaa
    10201 acgccgtacc cgatgccagg cttgccacgg taccaaccat cttttcgctg aattgcttcc
    10261 cgaaaatcag gttgatgagc aggccaatga cgggtgcaaa gaccagccac ggggccagaa
    10321 agaaaaatcc tgtgctaact gattcgctta agtgcatggt ttctccgtaa ttggtaattg
    10381 gagattagta atgactaatt accaattact ttttactttt atcccttcaa ggaattcaac
    10441 tcatcaatat tgatattctt gcgtgccttg aagatttcca cgatcaaggc gaggccgacc
    10501 gccacttcag cagcggctac tgcaatgaca aagaaaacga agatctgccc gctgaagaaa
    10561 ggctggccac cggcgatctc gtaggaacgg ccaaacgcca cgaaggcgag gttgcccgca
    10621 ttgagcatca attcaacgga catgaaaatg atcaacgggt tgcggcggat gagtacgcca
    10681 agcgcaccga tggtaaataa aacggcggaa agcgccacgt aataattgac agggaccatt
    10741 atttttgctc tctcttttcc tgcttggtca aaacgatcgc gcccaccatc gcgaccaaaa
    10801 gcaaaatgga tgtaatttca aaaggcagga gatactgatt gaagagggac attgccattt
    10861 cacgcaggtt ttccatggag ttgagggcgg catccggtgt tgagatgaat ttatcggtct
    10921 gcgcacggga aatgaccaga tacaccgatt ccacgaccag cacaacagcc agggtgtaag
    10981 ccagcggctt ttgccagggg agcgactggg agggtgcaag attctccgca ccgagcagca
    11041 tgatcacgaa caggaacaac accatgatcg cacccgcata caccgtgatc tgcgacattg
    11101 caatgaaagg ggcgcccagc aaaaggtaga acaccgccac agtaacaaaa ttcaacacca
    11161 ggtacagtgc agaatacact gcattgcggc tgaagagcat ccccaacgcg cttgaaatgg
    11221 cgatcaggga aaggacgagg aaaacgatta tgtcagaatt cataaaactc catgtacggt
    11281 ttgcaatttt ggatttacga gattacggtt cgtaaatcgt ccatcgtaaa ccgttaatcg
    11341 gatggatttt gcatctcagg cacggagcgg gtgaacacac cgggttccac tttgcgcggc
    11401 gtgatcatat ccacagtgcg gacgggttcg agcagcattt ccttcgggta gatggcctgt
    11461 cggcggtcgg tgaaagaaag ctcgtaatta tcgccaagca caatggcctc ggtggggcag
    11521 gcatcttcgc aaaagccgca gtagatgcag cgcaacatat tgatctcata ggtggatgca
    11581 taccgctgtc ccggcgagaa gcgcttttca tctgtattct cagccgcttc cacaaagatc
    11641 gcatccgcag ggcaggctgc ggcgcacagc gaacagccga tgcacttttc gaggccgtta
    11701 tcgtaacgct tcagcacatg gcggccgcga tagcgcgggc ggacagggcg cttttcttca
    11761 ggatattgat aggttacggt cttctcaaaa aggaaggaac gcagggtttc gcccaaacct
    11821 tttgaaagct cgataatggg atcaaatacg cccatgagat tttctcctca agtcactgtt
    11881 gataaaaaca accgccacca gcacacaaac gatcgaaagc acaggaatac cccaaatgat
    11941 catcgggttg ttcaattcct gagccagcaa aatacctgtg gcagtgataa aggcaaggat
    12001 cagggataac ggtaaaagca ccttccaacc gaaagacatc aaacggtcat aacgaatacg
    12061 gggccaggtg gcacggatcc agatcataaa gaacaacagg acaatgacct tcaacagcag
    12121 gtagatcggg ccaaggaacc agaaacggtc cacactaaaa atggtcccct gcaaaaacca
    12181 gaattcgcga tagccgccaa agaacaaggt ggccgcgatc atgcagatga cgatcatctt
    12241 ctggtattca gccatgaaga acaaagcgaa tttcataccg gagtattctg catgataacc
    12301 agcggtcaat tcctgttcgg cttcgggcat atcaaaggga gcacggttca cttccgccag
    12361 ggttacgatc atgaagacca gcgcacctgc aggttgaatg accgcaaacc acatcccttt
    12421 ttgggcgttc acaatatcgt tcaaattcat cgaatcgccg agcaaaatgg cgatcacaat
    12481 gcacaggccc agcgaaagtt cataagagat catctgtgcc gaggagcgca caccgcccaa
    12541 catggcgtac ttgttattgc ttgaccatcc cgccagcaca atgccataca ccgcaatgga
    12601 tgcaatggaa gtcaggtaca acacgcccac attcagatct gccacataca gggtgatctc
    12661 acggccaaac aatacgaact tcgagcccaa tgggatcacc gccgccagga tcaaagaggg
    12721 caccacggtc aacacaggcg caagcacaaa cacaaccttg tacacacgtg atggggtgaa
    12781 ttcttccttg aagatcagtt tcaccgcatc ggcaattggc tgcaacacac cttgaggtcc
    12841 agcacgattc gggcccacac gcacctgcat acgcgccagc gcgcggcgtt catacaaggt
    12901 caaatacgca aagcccgtca aaaggatcag gcagaggata aatcccttta atacccattc
    12961 aagccagatg gtccaatcca tatcacttca ccttccctgc aagctgaaca cgcgccacaa
    13021 caggttcgcg gatcgcaatc cccatactgc gcggcaccag tgcaacgcct gccgagatcg
    13081 tatcgtccaa tttcacaacc acttccgcat tcactccgtc gaaactgagc ttcacacgtg
    13141 tacccgcttc cacgcccaaa ttctttgcag ccattgggtg cagggtcacg gttggctcac
    13201 caacccactg accaagcagc ggcgccgatg aactgatggt cacgccatag tcatacaacc
    13261 tggtgacagg cacagccagc aattcattct cttttggacg aagagccgct tctttcctga
    13321 ccttgggtat gcgtacggtt ccaccggctt gagcggtcgg gacgagctgc acacccaacc
    13381 cctgtgtatt ctcgtaagtt gtgccgccgt agtacaggtc accacgccca acgatcggcc
    13441 attgtccgtg cgtttcggcc agtttggcat aactcaaatc ggcaaaagac tcaacagaat
    13501 gtgaaagaat gtcaaacacc acagaagccg atgacccttc gaggatcacg cccatttgtt
    13561 tggcgatctg tgatgtgatg gcaaaatcag gttttgcatc acccttcact ggcacagccg
    13621 cataaaaacg ctgcacacgg cgctcgccgg aagtgaaagt accttcgcgt tcggtgtagg
    13681 cctgtgccgg gaaaaccaca tcagcgatct cggtggtggc tgtttcgaga atatcctgca
    13741 cggctacgaa cttcgcaccc ttcaaagcct tggccaacgc agggtcatca ccaacagggt
    13801 ctgccccaac gatgtaaact gcctttcctt tgagagcctt ctccaggtca ggttcggggc
    13861 ggaaaccaat ttcccaggca ccctgatcgt tcacacgttc ccatacagcg accagtccat
    13921 tatttggttt accaacatgt tcggtgtctt tcaaaagcgt ggcacaggca gaagcaaggc
    13981 tggccgtgtc attcaaacca agtccatcgt tgccgtagaa aacaacggca ttctttgcct
    14041 tggcaaattc atcagcgatc ttgcctttgt tgcttaagtt agccactgcc tttgattcat
    14101 caccctgtgc ataacggatg acaaacttgg caaatttatc cagtttggtt tcgcgtgcat
    14161 tggcgacaat gagggttgct ccacggtcag cggcttgttt tacgcgcagc caccaaatgg
    14221 gggcttcctg atacaggtct gaagccacta caagaatggc atcacccttg cccatcttac
    14281 cgatgttcgt tccctgccca acaccaacaa gtgtggtcaa atctccgccg cccatgttgg
    14341 aatacaaaat ggcagtacca ccagccgtat cagccagtga cttcaggttg aacagatctt
    14401 cattggccaa acggcccgat gccaacacca cgaaatcctt tttcgatttc gtaaagttct
    14461 cggccgccat tttagttgca gcatcccatg aggcacgtgc cagcttttct tccttgcgca
    14521 cgagcgggcg ggtgaggcgt ttcttgcttt cggtaaagtg ataggtaaag cggcccttat
    14581 cgcacagcca gatttcgttt acttcttcat tctgtcgggg catcacacgc ttcacgacca
    14641 catcaccgcc agccttggcc tcacggcgtg tgttgagcgt ggtattgcag cccaccgggc
    14701 attgcgtaca aatggaagcc tgtgctttca actcccacgg gcgcgcgcca aaacgaaaat
    14761 cggcagtggt caatgcgccg acggggcaaa tgtcagtggt gttgccagag aagatggaat
    14821 cgaaccccgg ttctgagagt gaaatgatct gcgtgttacg tccgcgctca tcgaaaccga
    14881 gtactgcttc accggccact tcatcctgaa aacggataca gcgtgcgcac tggatgcagc
    14941 gttcgcggtc aagccagatc aagtcgccaa gcggaacctg cttctcgaaa tgcgctttct
    15001 catcataaat aaagcggctc ttgcccggac cgaaagccat ggtcaggttt tgcaatgggc
    15061 attcgccacc cttgtcacag atcgggcaat ccaatgggtg cgaagtaagc aaaaactcca
    15121 ctgtaccttt ttgcccatct ttggccttgt cggttgtggt catcaccacc atgccgtctg
    15181 aaacgcgatt ggtgcaagcc gtttcaagtt tgggcatgaa ctggatcttg ggctggccgt
    15241 tttccatcac cacctgacca gtggcacgat ccaacatggg acggcccacc tccaccagac
    15301 acatgcggca cataccggct ggctccagct tcgggtggtg acaaaatacc gggatgtcga
    15361 tcccagccat cttggccgca tcagcgatca acgttccatc gggaacggtt actgttattc
    15421 cattaattga tagcgtgact tgtttcgtca tgcgtgctcc attaagcctt taccgctttt
    15481 ttgaaatcct gcggaaatct ttcgataccg gtcaccaccg ccatggtgga gaattcacca
    15541 agtgcgcaca ggcatttgcc ttgcatttgc ttggccacat ttagaagcag gtccacatcg
    15601 ttctgcacgc cctgaccgga gtgaatgcgc tcggtgagat gctgcatcca atagttacct
    15661 tcgcggcagg ttgtacactt accgcacgac tcatgcttga agaaatggat cgtcttgttg
    15721 accacccaat cgatatccac agtatcgtcc aacacaatga cagaagcaga acccaggtcc
    15781 gcgcccaatg tgcgtacggt ggcgtaatcc atcggggtat cgagtacttt atcatccacc
    15841 acaatcagtg aagaagatgc gcccgcaggc atgatcgcct tcacaggtcg gccatccaaa
    15901 acaccgccgc catgttcgta gatcaactgg cggaaggtcg tgccgaaggg cagttcataa
    15961 ttaccgggtt tgcgcacacg tccggagagc gagaagatct tcacgcccgc gctatcagcg
    16021 gtgcccatag acttgtacca ctccgcgccg ttcgccatga taagggggac gttcgtaaaa
    16081 gtctccacat tattgatgat ggtgggcttg ccgtacaaac caaaggaagg cgggaacggc
    16141 ggacgcacac ggggctgtcc gcgtttgcct tcaagcgatt caagcagggc ggtttcttca
    16201 ccgcaaatgt atgcacccgc tcccaagtga gtgtagatct tcagggagta ttcagtgccg
    16261 aacaggttct caccaaggaa acccgccttt tccatatctg cgatcttttc atcaaggaaa
    16321 gcagccacct gccagaattc accgcgcagg taaatgtaag ctacattggc gccaaccgca
    16381 taggatgcga tcgccaaccc ttccaaaaac tggaatggat tgctttccat gatctcgcgg
    16441 tctttaaatg tgccaggctc agactcatcg gcattggcaa ccacataatg cggccaattc
    16501 ttggtatcaa tgaaagacca cttcataccg gtggggaaac ccgcaccacc gcggccgcgc
    16561 agacccgagt tcttaaccac atcggtcact tcattcggct gcattttgga aacagccttc
    16621 ttgaatgcgt caaaaccgcc gttcttttta tagacttcga tcttgtttag atcggcaata
    16681 tcgcgatgac gtaaaagaac gttagccatt atttcacccc cccatcagat ttcttcaggg
    16741 cttcgatcaa ttcaatggtc ttatccacgg tcatgttctc gtgatattta attccatcag
    16801 ggccctgggt ttggaacatg ggcgctttgt cgcaggcagc aagacatgtc acagcttcga
    16861 gggttaccaa tccatcgggt gtggtttcgc cgactttgat tccgaggttg ctgcatagat
    16921 ttttcatgaa atcatcggca ccccgtaaag cacagggcag gtcagtacaa atttgcatac
    16981 ggtatttgcc tgctttttcg tcgtgataca gcgaataaaa gccgacgatc gaggcaactt
    17041 ccgtctcggt gatgtcgagc atgcgggcaa tatcctgcat cgcagctttg ttcacataac
    17101 cttcctcgcg ctgggcaagg tagaggagcg gcatgaccgc cgagcgtttg tgctccggcg
    17161 gatacttgga caatatttgc ttaacttcct tcggatagga tttctcaagg ctcatctgtc
    17221 aatatctccg aggacaatat caatggaagc aagaattgca acaaggtcag ccacaaggtg
    17281 acctttcaca atctgcggaa caacggcaag attatcgaaa gaaggtgtac gcaggtgcat
    17341 gcgcaatggc ttcggaccac catccccttc aataaagaga cccaactcac ctcgtggaga
    17401 ttccacggcg ttgtagatcg aagcttgtgg cgccgagaaa ccttccgtcc acaacttgaa
    17461 gtgatggatc agagcttcca tgctgacacc aatttcagag cgcggcggtg ggacaaactt
    17521 tcggttttca gaacgaacag gaccaagcgg caatttattc aaagcctgtt caacgatctt
    17581 caaagactcg tgcagttctt caatgcggac aaggtagcgg gcgtatgtat caccctctgt
    17641 acgggtgggc acgttgaaat catattgttc ataaccgcag tacggacgca ccttgcgcaa
    17701 atcccaatcc acacccgatg cccgtagtgt aggaccagtt accccgtagg aaagagccgt
    17761 ctctttactc aggtagccga tatccaccat acggtcaata aaaagcggat tcttggtcaa
    17821 taacgattcg tactcggcaa ttcgtttcgg aaagatcttg ataaagtcgc gcacggcctt
    17881 ttcaaattcc acgggcacgt cacgccacac gccgccgggg cggaagtagg tggtcatcat
    17941 acgttgaccg gagacaagct cgaaaatatc aaggatctgc tcgcgttcgc ggaaacaata
    18001 caggaacatc gacatcgcgg caaggtcaag gcctgaagta cccagccaga ccaaatgtga
    18061 agcgatgcgc tgcaactcaa caaggatcac acgcaacgcc tgcgcacgca cggggacatc
    18121 aaggtccacc aatttttcca cggccataca atacgccagg ttgtttccca tcgtattcat
    18181 atagtcaagg cggtcggtca tcacctcggc cttttgatag gtcttggctt ccatgttctt
    18241 ctcaacaccc gtgtgcaaaa agccgatatc gggaatgcag ttgacgacaa tttcgccatc
    18301 cagttcgagc aaaagccgca aaacgccgtg ggtggaaggg tgctgcggac ccatgttcaa
    18361 aagcatggtc tcgccggtaa gcgcccgctc agatacaaga tgcttgaatt tttccaggct
    18421 gacttcttca atctgactca taactattcc tttacatacg gcttgcgcag atcgatctca
    18481 tcgaagttga aggtgaattg cggctcttca tacccaagcg gaaaatcctt gcggagcggg
    18541 tgccccacat gatcttcggg catcaagata cggcgcggat cgggatgacc ttcaaattcg
    18601 attccgaaca tatcgaagat ctcacgctca cgccagttcg ccgacgcata tacaccggtc
    18661 actgtcggga ccttgggact gctcccattt acagcgactc tgacctgcac cgaaagattt
    18721 ttgacaagcg aagtaagttg atagatcaaa tggaaacgcg gtgtttcgct ggggtaataa
    18781 tccaccgcgg tataagctga cagcaattca aaaccctgct cgtcgcgtag aagggtcaac
    18841 gcctccacga tctgctcggg cttcacgaac aaatgcactt catcgcgaaa ctcttcacag
    18901 cctgcgccga atttgtcttg aattgttttt acaacgggtt cgagattttt attcataaaa
    18961 aaatattcct taagacctga cagattgggc ctgtcaggtc ttatgaatga ttattttgcg
    19021 gccagatcct tgaatttttc acctttgacc ttttcataca cggtcaacac accatgtatc
    19081 aaggcttccg ggcgcggcgg gcaacccgcc acatacacat ccacaggcac aacttcatcc
    19141 acaccctgat agatggcata gttattgaag acaccgccgc aggaggcgca atcgcccatg
    19201 gcgatcaccc atttgggctc aggcatctgg tcatacaaac gacgcaggac gggtcccatt
    19261 ttcttcgaga cgcgtcccgc cacgatcatt aaatccgact ggcgcgggct ggctcgcatg
    19321 agttccatgc caaaacggct catgtcataa tctgaagcct gtgccgccat catctcgatg
    19381 gcacaacaag ccagaccaaa caacatcggc cacatggcat tcgtacgcga ccaattgacc
    19441 acattctcca gcgtggtggt aaccacaccc atatttccaa gtttctgctc tactcccatt
    19501 caagggctcc tttcttccat gcgtacacat aaccgatcaa taagataacg ataaacacga
    19561 tcatctctag caggccaaag atgcccagct tgcgaaatgc gatcgcccag ggtagaaaaa
    19621 acacaacttc aatatcaaaa aggatgaaca acactgcaat caggtaaaaa cggaccggca
    19681 tacgccgcgt accttcacca tatggattca tacctgattc ataaggcatg tctttggcgg
    19741 cagtcttttt cttcgggcca aacaattccc ccaaacctat cgcgatgaaa gcaatcaatg
    19801 tggcaaccgc aatcatcacc ccaatggcaa tatactccag caacataaac acctctttgg
    19861 tgtggaaaat cagtgcgtga aatctggcac gttcggcgta gtatatcata agagactttc
    19921 acgagccaga caaagtgtca cttttcacaa aaatgatgat aattgtcata atgggaatag
    19981 acaagcaata taaataaaaa cagaaggatg aaaacgattg ttttcatcct tcatgcttca
    20041 aaattcgttc tttcccctaa tctcccaggt aatctcgcag ttccctgctc ctggcggggt
    20101 gacgtaactt ccgcagcgcc ttcgcttcga tctggcggac gcgttcacgg gtgacatcaa
    20161 aatagcggct gacctcttca agggtatgtt cccttccatc acgcaggcca aaccgaagtt
    20221 cgagcacatc acgttcacgg tctgacaacg actcgagggc gtgctgcacc tgctcgcgga
    20281 gcatttcgcg ggcagcggca tccatcggcg acggggtgtc ttcgtcctca atgaaatcgc
    20341 ccaatgaact ggaatcttca tcccccaccg ggccttcgag ggaaacgggt tcttcggcag
    20401 aacggagtac cctgtcaatt ttttgtgtgg cttgttcaag ttgtttttgc aggtcaggtt
    20461 tgatcggcct gccttcggca tgtgcccgaa gaatggcttg cacatcggaa gcagagagaa
    20521 aacccgcttc catggccaat tcctcgctgc tggggtcgcg gcccaaaatc tgcgtaaggt
    20581 ggcgctgcgc tcgcaaaatg cgcgaaatcg actcgaacaa gtgcacgggg atgcggatcg
    20641 ttcgggcctg ttcagcgatg gagcggttga tcgattgacg gatccaccag gtggcatagg
    20701 tgctgaattt gaatccacgg cggggatcaa acttgctgac cgcacgcagc aggcccatat
    20761 tgccttcctg aatcagatca agcaaagaca tgccgcggcc aagataacgc ttggcaacgc
    20821 tcaccaccag acgcaaattt gcacggatga gcgcctgatc cccttccaca gcacgggcgt
    20881 ggatcgcgtc catttcggcg agcaactcat catcgggggg caggttgcgg tagagcgtgc
    20941 gttgcccggg cagcgcattc tgggcgcgga catttgtcag cagccactct gcatatttaa
    21001 acgggagcag gtacaggtta agaaagacac cgtaggcatg tcgcgcgaaa ttatcccaaa
    21061 ggcggtccac accccaatgg ccgttatcca gataagcgcg gaaataagaa ggtgaatcga
    21121 attgccagcc cgagtgcagc gattgtgact cggccaccat cagggccagg ttggggagtt
    21181 cacaatcgag ttgttcggta tcttcgatca ggcggtccca cgaagtggag agttccacca
    21241 gggcagtgtg ataaatggat gaggccagcg aaacgccttt gcgttgtttt agttttcgca
    21301 gcgcagtaat atggcggtcg gcttcgctaa gtgtagcgag acgaaactcg ctatctgaat
    21361 caagcagttt gaccaggccg atctcgcgca ggtacaaacg cacggggtct tcagaaagtt
    21421 ccgattccag ttcatgggaa cgcaggggca gaatttcttc aagctcatct ggctgaacga
    21481 gcaaggtctc atcaggctcg atgacctcat cggctgcggg gtcaaacaac agattgtttc
    21541 cgttggaaag cgaatccagc acatcgcttt ccgggaattt catgcttttt tctttcgctc
    21601 tacgcccctc tggtttttca gcgtttttta tttttggttt tgctactttc ccgtgcttgg
    21661 ttttggattt ggattttacg ggcatcggtt tcttctccca gatggagtct ctatcgtttt
    21721 gaggatagct ttctttttgc ctgatccaaa ctgtgaagca atcttgtcat ttgcaaggtt
    21781 tgctcctgat acagcttcat atttgccccg ccctgctgtt ggtcttcctc ctgaagaaaa
    21841 cgcaattgat tgacattcaa catcgcattt gcacggcgca agtccatgaa gcgcgccagc
    21901 aggtcatcga ggatcttgtc ttccacaggg tcaagagcat cggaacgggc gaccaattcc
    21961 ttcgaaagct cactgaccga ttccggcacg tgggccatca cataatgatg ataatccttc
    22021 tcatcctgtt caactgccat gcggacaaca ccgaacaaca actgatggtc agtatactcg
    22081 aaatcttcca cagccaatgc agccaggtcg gcttcctgca tgcgtttatc gagacggtac
    22141 agcaactcag gcttgcgcag caaaacactg aggcaatagg attcgatctt caaagtggga
    22201 ttgaccgcca caatgccaga cgacacaacc ttgtgacttt gctccacact ccgcggcttg
    22261 cgcctcgcca ctggaccctg cacctgcgtg cccgttaata cgctttcatt tacccgcaac
    22321 attcgcgcca gcgcctgacg ataggtatcg cgctccatgg cgctgggcag gtcttcgatc
    22381 agcggaagca cctgtgcggc gatctggttc ttcactttgg catcgttcag gttctgacca
    22441 gccgccagcg aatccatcac atgggtgacg atgggcttgg cattctcgat gagcttcttc
    22501 cattcttcct gatcgcgggc tacgatctca tcggggtcaa ggtcatcggg catggtcgcc
    22561 acacgcaggt ctgcttgcag gcgggcctca tttttcagca ggccgcgtgc atcgaagcca
    22621 agttcgcctt cgcgatccat cgcagagcgg gcggcatcaa gtccgcgcag aacggctttc
    22681 tggcctgccg catcagggtc aagcgccagc acaatgcggc gcgtggacct cttcaacagg
    22741 cgcagttgat cttcagtgag cgcggtgccc attggcgaaa cgacattttc atagcctgct
    22801 tgatgcaggg caatgacatc gagatagcct tccacgatca cagcctgatc tgccgcacga
    22861 atgggcttgc gggcgcggtc gaggccgtaa agcaaacggc ccttggaaaa aatgggcgtt
    22921 tcgggtgaat tcaaaaattt ggggacgtcg ttcggatcca cgatgcgcgc gccgaaacct
    22981 gccatctttc cattctcatc gcggatggga atcatgatgc ggttgcggaa tttatcgtag
    23041 gtgcgggctt cgtcattttc acggacagtg accatgcccg agtcgatcag atcctgctgg
    23101 ctgtagcctt tggagaggaa gtgcttcaac aggttgtcgt atccgggcgg ggcataaccc
    23161 aggccaaagg tttcgatggt ggagtcggtc agtccgcgct tttcatgcag gtaggccaga
    23221 attgcagggg cgccaaaaag ctgggtgcgg taataaacga ccgcatcttc cagcagtttg
    23281 cggagatggt cgtaggcttc tttttgttgg ggtgtttcgc gctgatactc tttgatctcc
    23341 acgcctgcac gcagggccag tttctgcatg gcctctttga aatccaggcc gtcgcgcttc
    23401 atcacgaact tgaaaatatc gccgccttca ttacattggc caaagcagcg ccacgtgcca
    23461 gattcaggcc agaccacaaa ggcgggcgtg tgtttgttat cgtggaaagg gcaaaagccc
    23521 gtgtagttct tccccgcatg acgcagtttt accccggcct cggaaacgag gtcaacgata
    23581 tcaatacggg ctttgatctc atcaatggtg gacatggttc tcctttgaat tatacttgcc
    23641 ccctatgaat tcaaggctta tcaaaaacaa ggtgtttgcg cttccatttt tggcggtcta
    23701 tgcgtggctg gtgatccgca cttcgtgggt gggggacgat gcgatcatca ccttccgctc
    23761 gctggaaaat ttcctgcatg ggtatggacc ggtattcaac atcggcgaac gggtgcagac
    23821 gtttacgcat ccgttgtggt ttttgttgca ggcggcggcg ggggctgtgt tcaacctttg
    23881 gaagagcaat ccctttggtc aggggcagat gtattttctg aatgtcttca tttccatcgg
    23941 catctccctg ctggccgcgg ccatctttac ttttaatgaa acgtcctctg caaaaggggc
    24001 ggctttgggg cttttgatct tttcggtctc caaatcattt gtcgattatt cgacctcggg
    24061 tctggaaaac ccgttgaccc atttgattct gctgttattc accttgacct atgttgggcc
    24121 tgccgaaaac tcacagcgcc gactctacct gctggcaacg ctggcagctc tcggcggggt
    24181 ggatcgtctt gatacgttgt tgttctattt cccggcgctg gtcctgctgt tttgggagag
    24241 tccgcacaaa gggaagacct tcctcacgct tgggtttggg tttctgccct tgatcgcctg
    24301 ggaattattt tcgctgttct attatggtgt gccgttccca aatacggcgt atgcaaagat
    24361 caacacgggc attcccgaag cgagtctttt catgcagggg ctgttctatt atttgaattc
    24421 gttgaagctc gaccccatca cattgctgac catctttacg gtttcggcgg cggggtggat
    24481 gagcaaaaac aaaaagcatc aggccatcct ggctggggtg ctgctgtatc tgctttacac
    24541 gatctacatc ggcggcgatt tcatgagtgg acgttacttc tccagtccgt tgctggtttg
    24601 cgtgaccctg ctggcaacca tggatttcga atcgccgaaa aaattcgggg ttgcagtcgg
    24661 aatcgttctg ctggtggggc tggctccgat cgcagtggtt gcagaacgtg cgcctgcgtt
    24721 tggcgtgggt gatgagggcg accaccgcgt atttgtggat gaacacggaa tttcagatga
    24781 acgcatcgtg tacaccgggt tggggctgtt tgaacgcttg aagaaaaaaa gcgctccttc
    24841 caccaattat gcgcgtgaca agtgggtgta cctgcccgat gaacctgtgc gggtgcgcat
    24901 tgtgggtccg ctgggtttga actcaatgac gcttggtccg aactttcatg tcattgacct
    24961 gaactcactg gccgatccat tgatgacccg catgccgctt tacgagatcg accgctggcg
    25021 catcggccat tttcggcaca tcattccaaa gggatatgaa gaaacgctca agagcggaga
    25081 gaatcgcatt gtgaacaagg atattgccca gtactacgac aagctctcct tcattgtcag
    25141 gggcgagcta tggagctggg aacgggtggt tgaaatttgg aatttcaaca cgggcaagta
    25201 tgattatctg ttggaaaatg tgactcccgc cgacgcgatg gaataacaaa aggccgctga
    25261 cgatgaagga cagttctttg aattcactgt aaaagattta cggccatccc atttctgaca
    25321 ggatggccgt aagttcaaaa gggtttttag tgcgaacacg ttgacaggtc agtacaggtt
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn acggggaaac cgtggggatt
    25501 agattcaaca gcctgcgcac gttcatgttc aacatggaat aatccatcat gccgacctgc
    25561 ccgaacgaga tattgccgtt gacatccaca aaatagttga cggggaacaa gttggtgccg
    25621 ccatatttct tgaaggtact ggctttcgag tcgatcaaca tgggataggt caggttgtga
    25681 ttatctcgat aggtgcgggc atggtcggaa tctccccaca catccactgc gaggacgacc
    25741 agtccgatat ctttatattg agtgtagata tcctgcatgg cggcggcttc agtgtcacaa
    25801 tagccgcagc cgttggccca aaagaagatg acaacaggtt tgcctttgta atcgctcaaa
    25861 ctgacctgat tgcccgtatt gacattcttt agcgtgaaat cgggagccgc catccccgtg
    25921 ccgaggccga cttgtgtctc tgcattgggg tcttcggtgc cgatgacatt ggaaatgggt
    25981 gtggacgtgg actcaatata acccagtgtg ctggtgggaa taagcgtggg gctgggagcg
    26041 ggagtccacg tggggggcaa accgatggtt ggaatggtgg tcggtgtttc cgtggggcca
    26101 aggaagatcg gctgtgcagt gggtgtggag atctgctgtc cacgcaggac gaagaagaag
    26161 ccgaccaaaa tcaggccaaa cacgcacaga aaaacgacga tcaaaacgat gtatttaaca
    26221 aaatcgttct tctttttcgg ggcgggcttt ttgcgcgcag gttgtgcggg tttgtccgca
    26281 gactgtgttt cggtttgaaa tggagaaacg cttgatgagt acggattttc ataggcatcc
    26341 tgtccgaagg aatgttcaaa agcatcggcg gaaggacgcg ggaggtcttc atcctcgggt
    26401 aaaaacaggg gcggaatgga ggaagattga tatgggtcct ggctgggagt gttcgagtag
    26461 gaacgctcga acgcgctcaa aggcggcgct gattgcaggt caaggtcatc ttcggggaag
    26521 aacggtgagg gtttgtaatt atcctgcgcg gcctgatagg aagagttgga aatattgaag
    26581 gcatcacggt catcgatact gtcatcgggc aaaaacgtgg ctgcctcagg cgggcggaca
    26641 ttcccttgac tctggcgaag gaaggaggcc tcgaaagcat ctgaagaagt cggcggggcc
    26701 gattcatcat ccaagataaa aggagcagtg atcgggtgaa gacgagacgg ttcctgactg
    26761 aatgtcgaat ctgatttcgg ggcgaggtcg ctttggagtt tcaataatcg gttgcgggcg
    26821 gggatattcc ccgggtcgag tttcaagacc cgttccacac aatccaattg ttgtttgggg
    26881 tcggtcaaaa tgaagctaag catccaccag gcacgggaag aatcgggatg gctttggaca
    26941 tactgcacca gcgcagtccg cccttcctgt tttttcccgt cacgcaggag ttgttcgatg
    27001 tattccaagc cgttgttttg gtcagccatg atgaaattat agactccgct tcagacaacg
    27061 caaagatgac atttatccta agatgcaagt acggcgggac caaatcatcc cgccgtactg
    27121 ataagctatt ggtacatggc gcagtataac gcctgcagac ggtcatgccc ctatgaaatt
    27181 cattacaaaa gccttttgtt tagaagcctt ccaacttgct cagatcagca atgccgttcg
    27241 aaagcccggc gatcttttgc agcaacccaa ggcggttttc cttgactgcc ttatcttcga
    27301 ccatgaccag cactttatcg aagaaagcgg tgatggcagg gatgagggcc gagacattgt
    27361 tcaggaagga atcaacatct cctgcaacca caggcggcag acggttgacc gcttcataca
    27421 accctttttc tgcttcttca acaagggatt tttcactcac cttgaaagtt tccttctggt
    27481 cacgggtaat gcggacacaa cgggcgaaac catccaaaat ggatgaccaa tcttcacgtt
    27541 cgacccaagc cgacagttga tccaccgcgc ggacagttcc cgcagggtta ttggattgcg
    27601 ccgcaagaac cgcctcaatg acatcgtgct tgaatcccat atcaccaagg gccaccttca
    27661 tacggcccgt gatgaattcg aggatctgtt tttgtacttc atcactgacc gggatcggct
    27721 gggtttgcgc agagagtttg accgctttcg caagatcgaa atccatcgga gacatcccga
    27781 ccgctggcga catcagcggc atgaccacgc caatggcagc gcggcgcaga ccaaagggat
    27841 cctttgcgcc tgtgggggca agccccgcac cgaacatacc gaccagcgaa tcgaggcggt
    27901 cagccagtgc aatggtcaac ccggcctttg tctgcggaac actttgatac tgctcgctga
    27961 tcgcagccgc aacttccttc tgttcgccgg agcgaagggc atattcaccg ccgataatgc
    28021 cttgcaggga ggtcatttcg gtgaccatct gtgtgacgag atcggctttc atgagatgcg
    28081 cagcgcggcg ggcgaagata gcctgatcgg cttcgagacc gagagagggg atcagctcat
    28141 tgaccagttt cacgatacgc tcagatttat ccaacatgga gccgagcttg gtgtggaagg
    28201 tgag
//
LOCUS       DAOQLC010000054        27978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_547_length_27978_cov_23.516492, whole genome shotgun sequence.
ACCESSION   DAOQLC010000054 DAOQLC010000000
VERSION     DAOQLC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_27978_cov_23.516492"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2510)
                     /locus_tag="PL000_10995"
     CDS             complement(<1..2510)
                     /locus_tag="PL000_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89470.1"
                     /translation="MKKSINPSRRMLVLVVVLALVGQACTLSLLENPFSSGTATQTPG
                     IAVPSATPQAMAQVNFVAVLPEPLQANETLIVTVMDEVTGLAFSDLQYPMTARDSLTY
                     TAVLPLPYNSIVKYRYIRQGNSKILEDSSLGAEIRYRMYYVAGPGEIQDIVSDWSDKS
                     YVRPTGSILGQVFNADTGSPVPNLLVNAGGVQQLTDSTGRFELAGLPVGTQNLVVYSL
                     DGLYQPFQQGAAVADNNATVVNLRVRPTQLVNVTFTVSVPDSTVPGVPVRIAGNILQL
                     GNTFADLQGNVSTNPDRMPILNLQPDGRYSATMALPVGTHIQYKYTLGDGYWNAEHKA
                     DGQWVLREFVVPAQDLAIQDSVETWTAGDAPILFEVTVPSITPPGDIIYIQFNTVGWM
                     EPLPMWPLGNNRWTYKLYGPLNILGSFSYRYCRNAQCGSADDVQTVGPAPAGRQVSTS
                     IMGQDIQDTVNAWKWYENPESVPLVGANITPRASGFIAGVEFQSTYHPNWSFYAPQAL
                     ANTQAIGSNMVVLTPSWTYTRVSPLNFTTVPGKDPLWIDSAIMVSQARALGLNVALFP
                     TPNFASLTTPTSISTNFWRDAPKDSVWWQAWFASYRTFAINYADMAAQTGAQTLILGG
                     EWIAPALPNGILADGTPSNVPADADAQWRSIIAEVRTHFKGQVFWGLPFTKAAIGVPL
                     DFLRDTDGIYLLWSAPLSANPSATKLDYANEAGRLLDNEVATLVSLLNKPLVLAIAYP
                     AASGSANGCVPNGQGGCLDWTELDRPNADIPSASLSLQNQADIYESMLAAVNSRPWIS
                     GFVSRGYYPPASLQDKSASIHNKPAADILWYWYPRLLGT"
     gene            complement(2544..3470)
                     /gene="tatC"
                     /locus_tag="PL000_11000"
     CDS             complement(2544..3470)
                     /gene="tatC"
                     /locus_tag="PL000_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC89471.1"
                     /translation="MRNFLSGVWKVITFPFRLVFNILAFPFRKIRQFNQFLNTEPEEH
                     PITEVFANLVTDADTRQMLWDQVEALRMHLLRAILSLVLTVSISFFFTNKVIEFLAGP
                     VGGLGNLKAIEVTETISVFMRVALMCGIALAIPYIAFELWLFAAPGLRSRERKMGLAG
                     IPLAAILFLCGAAFTFFLMLPAALPFLEGFMGVKTELRPESYFSFVIGLMFWIGVSFE
                     FPLIIYVLSAIGIIQPQVLAQQWRLAIVLIAIVAAMVTPTIDPINQGLVMAPMILLYF
                     ISIGLSYIAYAGRKRNNLEDQVNADKAEAGQG"
     gene            complement(3463..3858)
                     /locus_tag="PL000_11005"
     CDS             complement(3463..3858)
                     /locus_tag="PL000_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC89472.1"
                     /translation="MEILGIGTSELLFIVIIALIILGPKDMQKAGKTIGKWLRNVVTS
                     DGWKIFQQTSRELRTLPNKLIREANEELNQLGNDLNNASDPARNRPNNPIKPPHPQAV
                     PADFPPNKIAPPGVINTAKEKEGDPEQNA"
     gene            complement(3861..4322)
                     /gene="nusB"
                     /locus_tag="PL000_11010"
     CDS             complement(3861..4322)
                     /gene="nusB"
                     /locus_tag="PL000_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC89473.1"
                     /translation="MKPRTRARSIALQVLYETDMAENHLPGDVLQTRLNEAPLAEDLA
                     EFSRQIVFGVLPLTHDLDQLIAKYAPEWPLDQIAAIDRNILRIAFWEFAIQRETPVKV
                     AINEAVELAKLFGSDSAPRFINGVLGTLAENINEIQQVIKNRLAQETKLES"
     gene            complement(4327..4674)
                     /locus_tag="PL000_11015"
     CDS             complement(4327..4674)
                     /locus_tag="PL000_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNC89474.1"
                     /translation="MTENTQGKTTVSPDVLTTIARLAALEVPGVSRLAPISGGVNRLF
                     KRGVGDGIRIETEDNTVFVDLHLVLKAEVNIREVSRNVQHSVARAVQEMVGMDVGHVN
                     IHIEDIDYEDNEA"
     gene            complement(4764..5522)
                     /gene="fabG"
                     /locus_tag="PL000_11020"
     CDS             complement(4764..5522)
                     /gene="fabG"
                     /locus_tag="PL000_11020"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNC89475.1"
                     /translation="MSPTLSLENKVALVTGGSRGIGRAIALEFAARGAAVVVNYNKSP
                     ESAEEVVKQIQSTGGKAAAHQADVSDFKQAEALVKFSVETFGDLSILVNNAGITRDQL
                     IMMMPESDWDAVINTNLKSTFNCSKAAVKHMMRKRVGRIINMASVAGQMGNPGQTNYS
                     ASKGGQIAFTKALAREVAARNITVNAIAPGFVDTEILDAMTPETLEAALKMVPLGRKG
                     KPEEIAFAAAFLASDEAAYITGQVLGVDGGMAMM"
     gene            complement(5598..6461)
                     /locus_tag="PL000_11025"
     CDS             complement(5598..6461)
                     /locus_tag="PL000_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89476.1"
                     /translation="MQPIPSDEVLAKKFGQPRNELIQITDQHTLTVQAWLSSRSPQAA
                     RFNGKGIKASSTGCKIPLLNLALGCDFPKGISAIEIEEEVKAVIDFFAKRNVPWYWWL
                     NTKPSPMDIGQILEKYGLQYDASSLPAMIAPNRQDTTQFPALRKDIRVWRAHSVADLQ
                     AASKIRRLAFRFAEGQAMTYFEDMAADWLDDSSPARLFLAGESESEPMSIGAVIEGAG
                     IPGIYVMATLPDQHRKGYGKAILTRLLAEASASEGRIVALTASQAGAALYSQFGFLHL
                     FDFDFYSLPEE"
     gene            complement(6480..7154)
                     /locus_tag="PL000_11030"
     CDS             complement(6480..7154)
                     /locus_tag="PL000_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HNC89477.1"
                     /translation="MKPSFLSYLVSLLTPVALIGAALRILLSPIYYNVEYRMPYFPVD
                     EYGFTQQDRLQWAPYAVEYLVNSSDISYLGDLQFENGTPLYNERELSHMADVKNVVVG
                     ALRVWYLSLGLLALFAILAQRSGWMPDYLNGLRRGGMWMIGLAVALGLVAGIGITTNP
                     DVFWEFFALFHAIFFEGDSWLFYYSDTLIRLFPIRFWQDAFLWAAILALGGGAGLAFG
                     IRRRDS"
     gene            complement(7154..8704)
                     /locus_tag="PL000_11035"
     CDS             complement(7154..8704)
                     /locus_tag="PL000_11035"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNC89478.1"
                     /translation="MKKYLLFTVFVSGMTTLAAELAAGRLIGNVFGTSNLVWASIIGL
                     ILIYLTAGYFLGGKWADANPTPAAMYRILAWGAFTIGLVPYVAGPVLRAASTAFDALS
                     VGIMAGSFIAVLILFSVPITLLGAISPFAIRLSVDDTSKAGQTSGQIYAISTLGSFIG
                     TFLPTLVTIPTIGTTKTFLVFGLFLLFVALAGLGKFASKNEMLKLIWMPIVLAVVAIL
                     SAGQALKSNKGQVYETESAYNYIQVQEINGFTLLRLNDGQGVHSIYHPDTLQYNGPWE
                     QFMVGPFFYANRKPQDVQRMAIVGLAAGTAARQATAVYGDIPIDGFELDEKIVEVGQK
                     YFGLNLPNLNIHIGDGRLNLERSPYQYDIIAVDAYRPPYIPPHMTTQEFFEIIASHLT
                     EDGVLTINVGSVPGDRRLIDGLATTIATQFASIHIMDIPGSLNTMIFATKQPTTREDF
                     AANFIALSSDASVEPLLLNTMAVTYTNLTEGYTPTTVFTDDLAPIEWIVNDMVVSFVL
                     QGGLEFLQ"
     gene            8985..9425
                     /locus_tag="PL000_11040"
     CDS             8985..9425
                     /locus_tag="PL000_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89479.1"
                     /translation="MPVSSFIESDLSLLYVMVVGYCTIPELLEHEKMVSRDPARRPNM
                     KIVFDIFNADLDVDLNGIKAGVAYIHTVGRSGWELEPTAILTHNRILETLNQSYELMA
                     HEVVVRNKVFCSLTEALTWLGLSDHRRRVEEIRAQLLREFTLAA"
     gene            complement(9525..10382)
                     /locus_tag="PL000_11045"
     CDS             complement(9525..10382)
                     /locus_tag="PL000_11045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC89480.1"
                     /translation="MIPTLPFGRTGHQSTRTIFGAAAFWDTPQKDVDAAMDLVLQNGI
                     NHVDTAASYGKAEEVLGDWIRRHGKPFFLATKTEQRTKQAAYDQIRRSLDLLHVPQVD
                     MIQLHALHEEKDWDTAFGEGGVIEAVIQARAEGLVRFIGVTGHGVPVPEFHLRSLAQF
                     DFDSVLLPYSYIMMQNPRYAENFNKVLAVCKERNIAVQTIKGITRSPWKDVQQNRTTW
                     YRPLEEQADIDLAMHWVFGNPQVFLNTAGDVNLLPRILDAAHRFTTRPSEAQMQDLSQ
                     RLEMAPLFE"
     gene            complement(10402..11235)
                     /locus_tag="PL000_11050"
     CDS             complement(10402..11235)
                     /locus_tag="PL000_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89481.1"
                     /translation="MRRVFIRSFLLLMCILFSACQPVEFGYSPPIIVPVRVSINTAGE
                     IELGLSPELHTPIGTFDISFGADVYSLREEYDARLLIVILDDKATVYKLAEGKEFKIA
                     FDDSNKLYRSVNLKQEDDGDIILQLQSVSVAEEFQGSEEAQVTVEPMSTSTPEPPPCY
                     GVMVTPNDTSKGNILHIEGCPNWVFDSPPFAKGSYAISPNEKFLIYCTTVGDLYAIRF
                     RDQQTINLGNLRKDMPVFHTGDVSIGISISSGENQYFARIVDRISGQSLDFGIPLSIS
                     Q"
     gene            complement(11337..11492)
                     /locus_tag="PL000_11055"
     CDS             complement(11337..11492)
                     /locus_tag="PL000_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89482.1"
                     /translation="MFEQTFKNIDDKLWKDAGCSTPSATPSALAGTSPKYDGTSVVFG
                     GGRVGAF"
     gene            11693..11941
                     /locus_tag="PL000_11060"
     CDS             11693..11941
                     /locus_tag="PL000_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNC89483.1"
                     /translation="MVLTISNKGWVVIPAELRKKYNLHAGSEVVIVDYGGVLAIIPAA
                     RYPVKQGRGFLAGASSASTDLKKDRKLEKKRDARLANE"
     gene            11934..12353
                     /locus_tag="PL000_11065"
     CDS             11934..12353
                     /locus_tag="PL000_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC89484.1"
                     /translation="MSKTIDYILDAFAFMAYLENEPGAERVETILQDVEVGKARAYLS
                     IINLGEVIYTTERKYGLSKAQDTLALIQRMPIEILPADNQTVLAAAHIKANYPLSYAD
                     AFVVIAAQKLDGIIMTGDPEFQDVTELAKIEWLKQRR"
     gene            12358..>12938
                     /locus_tag="PL000_11070"
     CDS             12358..>12938
                     /locus_tag="PL000_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HNC89485.1"
                     /translation="MHIPKLYREEDSELIVKFLKQNNFPALVTFDGEKPIATHLPVEI
                     TQTENDGLTIYSHMSKANPQWKSFGGQEVLLIFQGAHTYISPRWYNHVNVPTWNYMMV
                     HVYGKVRMVEGDELYSLLGRLVRQHEADSNYRLESLPQDFVKKEMNGTVGFAVDVTRM
                     DAGYKLSQNRNDEDHANIIRELEGRGDVNSMEVA"
     assembly_gap    12939..13038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13066..13422)
                     /locus_tag="PL000_11075"
     CDS             complement(13066..13422)
                     /locus_tag="PL000_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC89486.1"
                     /translation="MTKHNQEIGAWGEGVAAEWLAAHGREVVARNIRTPYGEIDIVAR
                     QGDVTLFVEVKTLTSSANFFPEAQITARKREHMLNAAQHYCAEHEIDHWQIDVIAVEG
                     KPGSNPVVTHFENVVG"
     gene            complement(13419..14621)
                     /locus_tag="PL000_11080"
     CDS             complement(13419..14621)
                     /locus_tag="PL000_11080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC89487.1"
                     /translation="MRIGMMVDSYKPYVSGITNYVEVNKKYLEQAGHEVYVFTFGDLE
                     HQPDEPRVIYSKGLPLSDSGFYLSTRYSRTAKQLLQTMDVVHVHHPFLSGRLALRYCR
                     LLKIPIVYTNHTRYDLYAQTYLPIMPEEMSQGILQAYLPSFCNAVDLVVAPSAGMEKV
                     LRQLNVESKVEVVPNGVDLKNFQNANPLARAELGYKPDDILLVYAGRIALEKNLPFLL
                     KSFAGVAQAIPNAQLLLIGSGIQPYEEEIRNLIEEMNLTRRVRMVGRVAYEKLPAYLA
                     MCDAFVTASVTEVHPLSVIEAMGAGLPVMGIHSVGVGDTVVDGVTGLLATHDLPAYTA
                     KLTRLCLDPNLRTQLSDSARKASSAYAIERTTDMMLKHYERLVRESHPRKGHWSVRLR
                     KLLERITQ"
     gene            14708..14902
                     /locus_tag="PL000_11085"
     CDS             14708..14902
                     /locus_tag="PL000_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC89488.1"
                     /translation="MIAKLILRNKVYEVKAGMALVDALKKNNIVPESVIATRNGDLLT
                     DDEILRPGDEIKLIAVISGG"
     gene            14905..15846
                     /locus_tag="PL000_11090"
     CDS             14905..15846
                     /locus_tag="PL000_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89489.1"
                     /translation="MKCKKCGEKAAVQMRQHKLALCKDHYLEWVPEQTERFIKKYEMF
                     SRDEKILVAVSGGKDSLSLWDILVRLGYQADGLYLGLGIDGGINYSHESQRLSEEFAQ
                     RNNLKLHVVDIEKEYGQSIPVLAQNSHRGYGRPCAVCGLAKRHEMNRVARDLGYDVLA
                     TGHNLDDEAAVLFGNTLHWASDYLLRQGPVLPGSDGLARKVKPLCRFYEREMTAYAIA
                     RGIEYIYEECPFAEGSQSIFYKESLNLLETARPGAKLTFYLSFLEAKKSGNLFIEKNE
                     SFKNVHTCPKCGQPTTTPDFCSFCRMIEKSAATEEME"
     gene            complement(15894..16883)
                     /locus_tag="PL000_11095"
     CDS             complement(15894..16883)
                     /locus_tag="PL000_11095"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC89490.1"
                     /translation="MPQSLQNGEVLRDRYMIREQIGQGGTGSIYLAEDNRLEGRLCAI
                     KEVEHNRTLPTQFLEQAREQFFREASVLARLDHPNLPKVSDFFSEGPRDYLVMDYVPG
                     KDLRERMLDARRNKAFLSENEVLRWAVQISDALNYLHQQEPPIIHRDIKPSNLKITPS
                     GLIKLVDFGLVKILAPNEVTITIIQGQGTALYTPLEQYGGDDSHTDIRADIFSFGATL
                     YHLVTNEPPTEARKRFLNSRSLTPPREINPTLSSRVEKAILWAMSLHPDDRPKDVIEF
                     REALLGKKEAPEHITSSRSSFEIPSFSLFPQEQLATYTAMGLFVLGLIVTILR"
     gene            17210..17866
                     /locus_tag="PL000_11100"
     CDS             17210..17866
                     /locus_tag="PL000_11100"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC89491.1"
                     /translation="MDHEDEFPILVAQEGPLKGQRWSLSRALMVGRDPTCDINVQDRQ
                     VSRFHARITPTTEGVTVEDLGSKNGTNHNGVELTGPVMLQDGDLLGIAIAQQFLFLTS
                     DATMPLAESGARPGRLMMDNKSRRVWVNQQQVTPPLSAQQFKLLWLLYENQGQVISRT
                     DLVATVWGEDQMAGVSDQALDALIRRLRDRLASLDPTHQYIDSVRGHGVRLDNPPSGG
                     "
     gene            17873..18346
                     /locus_tag="PL000_11105"
     CDS             17873..18346
                     /locus_tag="PL000_11105"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC89492.1"
                     /translation="MDVQSEKLLVRILSTTRIAALGTIRDEEPRVSMVAYVAAHDYSA
                     FYVHVSRLAQHTVDMQKNKQVSLLISETDDGRADPQTLARLSIRASAEFMENGEPGYA
                     PVKKMYIEAFPESEPLFKMADFGLWRIKPKGARYVAGLAKAFNLTPETLQKVAAK"
     gene            18398..19207
                     /locus_tag="PL000_11110"
     CDS             18398..19207
                     /locus_tag="PL000_11110"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC89493.1"
                     /translation="MEQLKLLAVFAHPDDESMGMGGTLAKYSAEGVDTHLVCASRGEV
                     GWFGPEEQNPGPERLGQLREKELSNAVHELGMKSLHFLNYIDGSVDKADHAEAIGRIV
                     THIRRIKPQVMVTFPPDGNYGHPDHIAVGQFTSAAIVCAADASYVDAENLSPHRVSKL
                     YYMVDGENFINLIAPFMGDMTFPVDDQIRGEIAWKEWMITTRIEMSAHCNAAWKAIQC
                     HQSQLPTLGALAEMDADAAAAVLAMQGAFYRVFSLVNGGRGLETDLFEGLR"
     gene            complement(19277..19849)
                     /locus_tag="PL000_11115"
     CDS             complement(19277..19849)
                     /locus_tag="PL000_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNC89494.1"
                     /translation="MTTLAPTLSIRYFPRTATWLRDLLLIVFGSLFVAALAQVEIPLP
                     FTPVPITGQTFGVLIIGAALGSRRGAMSLALYLAEGIVGLPFFAGGAHGLSVVVGATG
                     GYLIGFIAAAYIIGLLAERGLERNARTSFIPFLVGTTIIYAFGITWLSILLGSFSKAV
                     ALGMVPFLIGDVIKLLAAAVALPAAWKIVK"
     gene            20042..20968
                     /locus_tag="PL000_11120"
     CDS             20042..20968
                     /locus_tag="PL000_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89495.1"
                     /translation="MKHFFDQAQRLPEDTSQNAVESFFRGQNASGLIEKQLHSGKSVI
                     LLFANGSPSAAYQLENGLGTFITHGEFSAVQNEIANVRALQLPDVAGRLTLLALESQI
                     GDELAMPDSVIWQAQLEKWKQARWSGLVEVESNDLQGLALFWQGELQASDVIFSTASG
                     FVTELPKAGIFPWKVTTYLPVPTAQGYQCAILRLGAVHWSFRILSRYQEMVGQKLLQT
                     MDRELNRQIQPWRWKIALTDSNMLDTHFFPYLMDAAHAYRALFMAMGAQMNFVIGSNL
                     TLRLLNETFEQIHPDERALLQSQRLIPAAFSE"
     gene            20973..21857
                     /locus_tag="PL000_11125"
     CDS             20973..21857
                     /locus_tag="PL000_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89496.1"
                     /translation="MSESTNQTGGNRLSPKTILRLYMAVSFFILLLILWTYYAEPLTE
                     SVSAYWNDKLLDTLILIPALGAAIAGVLVVRQFDAGEQPHRIWQAFTIGLWFWVAGEL
                     SGFVYDAIYIDLPYPEFSLIDVCWLAGYFYLGLSLYYQVRLVYSAQKRKGRLLYLALV
                     GLAFLIAALLTQLAAKAGLGGESAWIILFVSILYPVFDLAEGSAAIWLSLLFGRGQWS
                     RPWWGLILFALADSVDTFYWLGGYDFIAPVVQSVLDVISVAAYPASYMVAGLALLSNY
                     FILQYGVESGLLQTQKRK"
     gene            22008..24989
                     /locus_tag="PL000_11130"
     CDS             22008..24989
                     /locus_tag="PL000_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89497.1"
                     /translation="MSLKNSPQKVNILKGRDSAEFRKMFGNHPTPVWIHDRESFAILA
                     VNQALSSLLGYEPTELEGISLENIFPVEEVSSLYADLKLPRQIGHSPREWHLRSKSGQ
                     LISIDQTFSSFIYEGREAALVIVHLRTDQTLESRILGSRLRISEFAHSHSLDDLLQKT
                     LDEAELLTQSNIGFFHFVEADQKTLSLQNWSTNTLKNMCTAEGKWSHYSIDEAGVWVD
                     CVRFGGPVIYNDYPGLEHRKGLPPGHAPISRLLSVPVTRGGSVVAILGVGNKVEEYNE
                     SDIASVYLLADLAWDITVGKRTEHGLHQSEERLRLLFDKISNIAVQGYAPDGTVRLWN
                     QASEKIYGYTKDEALGRNLLDLIIPEEMRADVEASVKKMIGTMQPIPSEELSLKRKDG
                     SRVSVYSNHTVVEISGVGRELYCIDIDLSERKSFEEALRESEEKYRQLFEAESDAIFL
                     IDNETGQIFEVNSAATKIYGYTREELIRMRNVDLSAEPDNTRKAMQSKMVNIPVRWHR
                     KKNGVVFPVEISASHLMWKGRPIHIAAIRDITERKQAEDALHESEAQYRLMFQNNPHP
                     MWVYDLNTLKFLEVNDAAISHYGYSRDEFLRMTILEVQSVDSLAMLRAPLVDPFLQDY
                     RSGVWQHRKKNGMLIEVEITQHDTLFNGNAARLILANDITERKQAENALREKELRHRI
                     VADNTADWEFWRAPDGKFLYSSPSCREVTGHTMEEFISDDRLLERIIHPDDLATFNNH
                     RHFELTAKMSSEVEFRIVLPDGWVHWIGHVCQPVFDDQNSYLGIRGSNRNITARKQAE
                     EDLHRTKKSLEEVNSVLLAAIEREQRISRTDSLTETYNRRYFFEIATHECSVAERYRQ
                     PLSLIMFDIDNFKKFNDIYGHQVGDEVLRRIAQVAREQLRSADILARYGGEEFTVLLP
                     KSNTHEALIVAERIREKVAACKLDTGNGVVGITISIGVAEYFPDQQTLDDLIRNSDKA
                     LYTAKEKGRNQVSVYATGNTV"
     gene            25098..26459
                     /locus_tag="PL000_11135"
     CDS             25098..26459
                     /locus_tag="PL000_11135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC89498.1"
                     /translation="MVSTKTVPHTRVKAKIKKDDERLRLALRLSPSPMWIFDLESLLF
                     LAANRPAQLQLEYSLPALKRMKLSDIIPADEHPNLYLTLKQVGRFGHFPGRWHLLPKS
                     GQTISVSVNSSLVKFNGRNAVLVSAFASPFEGLLTENCSRYFALVANRISSAVISTDS
                     ALRIIQWNKAAEDLYGLTETEAMGCLIDEVCCTEFGEDQQSEARASLRTKKRWQGELK
                     QHHRNGAELWVSTSVTLLEDENGNFLGGITINHDITERKKVEDDLRHTKQVMEEINQI
                     LQHAFEREQLASRTDALTGIFNRRYFFEFIEYEVALSKRYQQPFSLIMFDVDHLKLVN
                     DTTGHQAGDEVLKQIVLLVQKELRSVDILARYGGDEFVILLPNSSVPDALILAERIRS
                     QIFAANLRVAGSSLQVTVSIGAADFNPSVSSSAELISHADEALYSAKQAGRNCILAYS
                     AKI"
     gene            complement(26456..27055)
                     /locus_tag="PL000_11140"
     CDS             complement(26456..27055)
                     /locus_tag="PL000_11140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89499.1"
                     /translation="MKTRWIQPVTLTGAHVVLEPLSLEHLDGIKEAVLDGELWKLWFT
                     SIPSPETAETYIRSALELRENAGWMAFVVREKATNKIIGSTRYCNVDEVNQRLEIGYT
                     WYAESYQRSAVNTECKYLLLTHAFEKLDAIAVEFRTHWHNHKSRAAIARLGAKQDGVL
                     RNHTKSANGVYRDTVVFSIINLEWPVVKQSLEYKMSKPY"
     gene            27135..27557
                     /locus_tag="PL000_11145"
     CDS             27135..27557
                     /locus_tag="PL000_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNC89500.1"
                     /translation="MNWHKTTIEIATSGKGLRDITPAIRAQLKEWQVQEGMCFLFLQH
                     TSASLIISENWDPTARADLETFMERLVPEQDSWYTHDLEGPDDATSHIRSMLTDASLT
                     IPVDEGDLSLGTWQGIYIFEHRARPHRRKLLMRVLSVE"
     gene            complement(27593..27919)
                     /locus_tag="PL000_11150"
     CDS             complement(27593..27919)
                     /locus_tag="PL000_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89501.1"
                     /translation="MEYWDVGDRVFAYWDDDDYFYPATILEMEEEDIYIRFDTGEEEW
                     TQADYLEEYATAAGEEVECKSAKDNQYYDVTVLNANGEQVEVEFDDGSTEWTTLNKIR
                     FYIEEE"
BASE COUNT         6784 a         6898 c         7440 g         6756 t
ORIGIN      
        1 gttcccagca ggcgcggata ccaataccaa agtatatcgg cggcgggttt gttgtgaatg
       61 gatgcagatt tatcctgcag ggatgcgggt ggataataac cgcggctgac gaagcccgag
      121 atccatgggc ggctattgac cgcggccagc atggattcat atatgtcggc ttgattttgc
      181 aggcttaaac tggcagacgg aatatcggca ttggggcggt ccagttcggt ccagtcgagg
      241 catccgcctt gtccgttggg cacacagcca ttggctgagc ctgatgcggc tgggtaggca
      301 atggccagaa cgagcggctt gttcaaaagt gaaaccagag ttgcgacttc gttatccaac
      361 aggcgcccag cttcgttggc gtaatccagt tttgttgcgc tggggttggc agagagcgga
      421 gccgaccaga gcaggtagat gccgtctgta tcacgcagaa aatcgagggg aactccgatg
      481 gcggctttgg tgaatggcaa tccccaaaag acctgaccct tgaaatgggt ccgcacttcg
      541 gcgatgatgg acctccactg cgcatctgca tccgcaggga cattggaagg cgtgccatcg
      601 gccagtattc cattgggcag ggcgggggcg atccactctc caccgaggat gagggtttgc
      661 gctccggttt gggcggccat atcggcataa ttgatcgcaa aggtacgata gctggcgaac
      721 catgcctgcc accagaccga atcttttgga gcatctctcc agaagtttgt gctgatcgag
      781 gtcggtgtag tgagagaagc aaagttcggc gtggggaaga gcgccacatt caagccgagg
      841 gcgcgggctt gtgagaccat gatcgccgaa tcgatccaca gcgggtcttt gccgggcaca
      901 gttgtaaagt tcaaaggcga aacgcgggtg tacgtccagc ttggggtgag gacaaccata
      961 ttggacccga tggcctgtgt attcgccaga gcttgcggcg cgtagaacga ccagttggga
     1021 tggtaggtgg attggaactc caccccggca ataaatccgc tggcgcgcgg ggtgatattt
     1081 gcaccaacca aaggaaccga ctcggggttt tcataccatt tccaggcgtt gactgtatct
     1141 tgaatatcct gccccatgat gctggtggaa acctgccgcc cggcaggagc agggccaacg
     1201 gtctgcacat cgtctgcgct gccgcattgt gcgttgcggc agtagcgata ggagaaggag
     1261 ccgaggatat ttagcggacc gtacagttta tatgtccagc ggttgttgcc aagcggccac
     1321 atcggaagcg gctccatcca accgactgtg ttgaattgaa tgtagatgat atctccaggc
     1381 ggcgtaatcg aaggaaccgt cacttcaaac aggatcggcg catcaccggc cgtccatgtt
     1441 tcaacgctgt cttgaatcgc aaggtcttgt gcagggacaa caaactcacg caacacccat
     1501 tgaccatccg ctttgtgctc ggcgttccaa tagccatctc cgagcgtgta tttatattga
     1561 atatgtgtgc caaccggcaa tgccatggtt gcagagtaac ggccatcagg ctgcaggttc
     1621 aagataggca tgcggtcggg attggtgctg acattaccct gcaagtctgc aaatgtgttt
     1681 cccaattgca gaatattgcc tgcaatacgc accggcacac cgggcacagt cgaatcaggc
     1741 acagacaccg taaaggtaac attcaccaat tgagttggcc gcacgcgcag attgaccacg
     1801 gtcgcattat tatctgccac ggccgcgcct tgttgaaagg gctggtacaa accatcaagg
     1861 ctgtagacaa ccagattttg agtgccgact ggcagccctg ccaattcaaa ccgcccagtg
     1921 gaatcggtca actgttgtac gccgcctgca tttaccaaca ggttgggcac aggtgaaccc
     1981 gtatctgcat tgaacacctg cccaaggatc gaacctgtgg gccgcacata ggacttgtcg
     2041 ctccaatcgg aaacaatatc ttgaatctct cccgggcctg ccacatagta catgcgatag
     2101 cgaatctctg caccgaggct gctatcttcc aatatcttcg agttaccctg acggatgtat
     2161 cggtatttga caattgagtt atacggcagc ggcaacacgg ctgtatatgt cagtgagtcg
     2221 cgggcggtca ttgggtattg caaatcgctg aaagccagcc cggtcacttc gtccatcacg
     2281 gttacgatga gggtttcatt ggcctgcaaa ggctcgggta acactgcgac aaaattgacc
     2341 tgtgccatgg cctgtggggt agccgagggt acggcaatcc ccggtgtttg tgttgcagtg
     2401 cctgatgaaa atggattttc gagcagggaa agtgtacatg cctgcccgac aagagcaagc
     2461 accaccacaa gaacgagcat gcggcgtgat ggattaatag actttttcat ttctctctcc
     2521 tggataaaaa gaacgcttcg cgcttaaccc tggcctgcct ccgctttatc ggcattgacc
     2581 tgatcttcaa gattgttgcg ctttcgcccg gcgtaggcga tgtagctgag gccgatactg
     2641 ataaagtaca acaggatcat cggcgccatg accagaccct ggttaatcgg gtcaatggtg
     2701 ggggtgacca tggcggccac aatggcaatg aggacgatag ccagccgcca ttgctgggcc
     2761 agcacttgcg gttggatgat cccaatggca gacagcacat aaataatcag cggaaattcg
     2821 aatgagacac cgatccaaaa catcaaaccg atgacaaatg agaagtagga ttccggacgc
     2881 aactctgttt tgacgcccat gaaaccttca aggaaaggca gggcggcggg cagcatcaga
     2941 aagaatgtaa atgctgcccc gcataagaat aagatggctg ccagtggaat gcccgccaaa
     3001 cccattttgc gctcacgtga gcgcaaacct ggagcagcga acaaccaaag ttcgaaggca
     3061 atgtatggaa ttgccaatgc aattccgcac atcaaggcca cacgcataaa aacactgatc
     3121 gtctcggtga cttcaatggc tttcaggttg cccaacccgc cgaccggacc agccagaaat
     3181 tcgatgactt tgtttgtaaa aaagaaggaa atgcttaccg tgaggacaag cgaaagaatg
     3241 gcccgtaaca aatgcatacg cagggcttcc acctggtccc agagcatctg acgcgtgtcg
     3301 gcgtcggtaa cgagattggc gaacacctcg gtgatggggt gttcctctgg ctcggtgttc
     3361 agaaactggt tgaattgacg gatcttccga aatgggaagg caaggatgtt gaagacgagg
     3421 cgaaatggaa aggtgatgac tttccacacg ccggagagaa aattacgcat tttgttcggg
     3481 atcgccttct ttttctttgg ctgtatttat aacgccgggg ggcgcaattt tattgggcgg
     3541 gaaatctgca gggacagcct gggggtgcgg cggcttgatc gggttgttgg gcctgtttcg
     3601 agcggggtca gaggcgttgt tcaaatcatt cccgagttga ttcaattcct cgttcgcttc
     3661 acggatgagt ttattgggca gggtgcgcag ttcacgcgaa gtctgttgaa agatcttcca
     3721 gccgtctgat gtgacgacat tgcgcagcca cttgccgata gtcttgcccg ctttctgcat
     3781 gtctttggga ccaaggatga tgagcgcaat gatcacaatg aagagcagtt ccgatgtgcc
     3841 gattccaagg atttccattt ttatgattcc aatttcgtct cttgggcgag gcggttcttg
     3901 atgacctgct ggatctcgtt gatgttttca gccaatgttc ccagcacgcc gttgatgaag
     3961 cgtggcgcgg aatcggaacc aaacagtttg gccagttcca ctgcttcgtt aatagccacc
     4021 ttgaccgggg tttctcgctg gatggcgaat tcccaaaagg caatacgcag gatgttgcgg
     4081 tctatggctg cgatctgatc gagcggccat tcgggggcgt attttgcgat cagctggtcc
     4141 agatcgtgag tgagagggag aacgccaaaa acgatctgcc gtgaaaattc tgcaaggtct
     4201 tccgcaagcg gcgcttcgtt aagacgcgtt tgtaacacat caccgggcag gtgattttcc
     4261 gccatatctg tttcgtaaag tacttgcaga gcgatggaac gcgctctggt tcggggtttc
     4321 atgctttcag gcctcattat cctcgtagtc aatatcttca atgtggatgt ttacatgacc
     4381 gacatccatg ccgaccattt cctgaacggc acgcgccacg ctgtgttgaa cgttgcggct
     4441 gacctcacgg atgttgacct cggctttgag gacaagatgc aagtcgacaa atacggtatt
     4501 gtcttccgtt tcaatgcgga ttccgtcacc aactccacgc ttgaagaggc ggttgacgcc
     4561 tccggagatg ggagccagcc tgctgacacc gggcacttcc agtgcggcaa ggcgggcaat
     4621 ggtggtgagg acatcagggg agacggtggt ttttccttga gtgttttcag tcattcctac
     4681 tcctgggggg aaccccttga ttgcttacag gttttcttat ctttttcagt gttgtcattg
     4741 caatgatcga cacaatttcc aaactacatc atggccatgc cgccatccac acccagcacc
     4801 tggccggtga tgtaggcagc ttcatcagaa gcaagaaatg cggcggcgaa ggcgatctct
     4861 tcgggtttgc ctttgcggcc caacggcacc atcttgagcg cagcttcgag ggtttcagga
     4921 gtcatggcat cgaggatttc ggtatcgaca aagcccggcg caatggcatt gacggtgatg
     4981 ttacgggcgg ctacttcgcg ggcaagggct ttggtgaaag cgatctgtcc acctttggag
     5041 gcggaatagt tcgtttgtcc ggggttgccc atttgaccgg ctacggaggc catgttgata
     5101 atgcgcccga cacgcttgcg catcatgtgt ttgacggcgg cctttgaaca attgaatgtg
     5161 cttttcaggt tggtgttgat gaccgcatcc caatcactct cgggcatcat catgatcaac
     5221 tggtcgcggg tgataccggc gttgttgaca aggatgctta gatcgccgaa agtttccacg
     5281 ctgaacttga ccagcgcctc agcctgtttg aaatcagaca catctgcctg gtgagcggcg
     5341 gcttttccgc ctgtggattg gatctgtttg accacttctt cggctgattc gggtgatttg
     5401 ttgtaattga ccacaacggc cgcaccgcgt gcggcaaatt cgagggcaat ggcacggccg
     5461 attccgcggg aaccgccagt gaccagcgct actttgtttt ccaatgataa ggtgggtgac
     5521 atcagttgac tcctgtattg aaattataca tcatgtgcaa taacccactg tctcctgttt
     5581 gggaacggca tttcttgtca ttcttcgggg agtgagtaaa aatcgaagtc gaataaatga
     5641 agaaagccga attgcgaata cagggctgcg cctgcctgac tggccgtcaa tgccacgata
     5701 cggccttctg aggcggaggc ttcagcaagc aggcgggtaa ggatggcctt gccatagccc
     5761 ttgcgatgtt gatcaggcag ggtggccatg acgtagatac cggggatgcc tgccccttcg
     5821 atcaccgcgc caatggacat gggctctgat tcgctttccc ctgccagaaa cagtcgggct
     5881 gggctggagt catcgagcca gtcagcggcc atatcttcga aataggtcat ggcctggcct
     5941 tcggcgaaac gaaatgccaa cctgcggatt ttgcttgcgg cttgcaaatc tgccacggaa
     6001 tgggcacgcc agacgcgaat atctttgcgg agtgcgggga attgcgtggt gtcctgtctg
     6061 tttggtgcga tcatggcggg cagtgatgag gcgtcgtatt gcaggccgta tttttccagg
     6121 atctgaccga tgtccattgg cgatggcttt gtgttcagcc accaatacca cgggacgttt
     6181 cgcttcgcga agaaatcaat cacagctttg acttcctctt cgatctcaat tgcgctgatg
     6241 cctttgggga aatcacatcc cagtgcaagg ttgagcagtg gaattttgca tccagtagat
     6301 gatgctttga tgccttttcc attgaacctt gccgcctgag gactgcgcga ggaaagccat
     6361 gcctgtaccg tcaatgtgtg ctgatcggtg atttgaatca gttcattgcg tggttgacca
     6421 aactttttcg cgagaacttc gtcagacgga attggttgca tggttgggcc tttgtcagat
     6481 caactgtctc tgcggcgaat gccgaaagcc agccctgcac caccgcccaa agcaaggatc
     6541 gcagcccaca ggaaggcatc ctgccagaag cggatgggga agaggcggat gagcgtatct
     6601 gaataataga agagccagga atcgccttcg aagaatatgg cgtggaagag ggcgaagaat
     6661 tcccagaaca catcggggtt ggtggtgatg ccgatgcctg caacaaggcc gagtgccaca
     6721 gccaggccga tcatccacat accgccgcgg cgcagaccgt tgagataatc gggcatccat
     6781 ccgctgcgtt gggcgagaat ggcaaacaat gcaaggaggc caagcgagag ataccagacg
     6841 cgcagcgcac cgacgacaac gttcttcacg tcggccatgt ggctgagttc gcgctcgttg
     6901 tacagcggcg taccattctc gaattgaaga tctccgagat aggaaatatc ggaactgttg
     6961 acaagatact caacggcata aggcgcccat tgaagacggt cctgctgggt gaagccatat
     7021 tcgtcaacgg gaaagtaggg catgcgatat tcaacgttgt aataaatggg ggaaagcaaa
     7081 atgcgcaagg cagcaccgat gagggcgaca ggggtgagca gagagacaag gtaggagaga
     7141 aaggatggtt tcattattgt aaaaattcca ggccgccctg caaaacgaaa ctcacaacca
     7201 tgtcattcac aatccattcg atgggggcga ggtcatcggt gaatacggta gtgggggtgt
     7261 agccttcggt aaggttggtg taggtgacag ccatggtgtt gagcagcaag ggctcaacgg
     7321 aggcgtctga tgaaagggca ataaagtttg cggcgaaatc ttcacgggtg gtgggctgct
     7381 tggtggcgaa gatcattgtg ttgagcgagc ctggaatatc catgatgtgg atggatgcaa
     7441 actgggtggc gatcgtagtg gcaaggccgt cgatcaggcg gcgatctccg gggaccgagc
     7501 ccacgttgat ggtcaggacc ccgtcctcgg tcaggtggga ggcgatgatc tcgaagaatt
     7561 cctgtgtggt catgtgcggg ggaatatacg gagggcggta ggcatccacg gcgatgatgt
     7621 catattgata agggcttcgt tcaaggttca agcgcccatc accgatgtgg atgttcaagt
     7681 tcggcaggtt taatccaaaa tatttttgcc cgacctccac gattttttca tcgagttcaa
     7741 aaccgtcgat gggaatatcg ccatacacgg ctgtagcctg ccgcgccgct gtacccgctg
     7801 cgagcccgac aatggccatg cgttggacat cctgtggttt acggtttgca taaaaaaacg
     7861 gaccgaccat gaactgttcc catgggccgt tgtattgcaa tgtgtcggga tgatagatcg
     7921 agtgtacgcc ctgcccatcg ttgaggcgca gcaacgtaaa gccgtttatc tcctgcactt
     7981 gaatatagtt gtaagcggat tcggtttcat aaacctgccc cttgttgctc ttgagcgcct
     8041 gccccgcaga aagaatggca accacagcaa gtacgatggg catccagatc agtttgagca
     8101 tttcattttt gctggcgaac tttccgagcc ccgcaagcgc aacaaaaagc aagaacaatc
     8161 cgaagaccaa aaacgttttg gtggtgccga tggtggggat ggtgaccagt gtaggcagga
     8221 aggtgccgat aaatgagcca agtgtggaga tggcgtaaat ttggcccgag gtctgacctg
     8281 cttttgaggt gtcatccaca gaaaggcgga tggcaaatgg tgagatcgcg ccaagcaatg
     8341 tgatgggcac gctgaacaga atcaacacgg cgatgaacga cccagccatg atgccgacgc
     8401 tgagcgcatc gaacgcggtc gaagccgccc tgagaacggg tccagcgaca tagggaacga
     8461 ggccgatcgt gaatgccccc cacgcaagga tgcggtacat ggcggcggga gtgggatttg
     8521 cgtcggccca tttaccgccg aggaaatacc ctgcggtgag ataaatgagg atcagcccga
     8581 tgatgctggc ccaaacgagg ttgctcgtgc cgaacacatt gccgatcaaa cgtcccgcgg
     8641 ccaattcggc ggcgagtgtg gtcatgcctg agacgaaaac agtgaagaga aggtattttt
     8701 tcatggatgt tccgcgaaat aggatggggc ggattataaa tgatgaatga tgagggatga
     8761 atgaggaaag agggggatag gatgtggaag ataaaaagag aaaaatacgg gaggatttat
     8821 cttttttgta attttgaggg cgtaccctat ggcggagagt actggttata attcattaag
     8881 tactccaagt tgcggcttgt acaaaacaga atagtttgtg atttcaagcc agtaacttga
     8941 atttcataaa tcttttattg ccacgaagga tttgaaaaga gaatatgccc gtttcttcat
     9001 ttatcgagtc agatctgagc ttactatatg tgatggtggt ggggtattgc accattccag
     9061 aactgctgga gcatgaaaaa atggtaagcc gtgaccctgc cagacgtccg aacatgaaaa
     9121 tcgtgttcga tattttcaac gcggaccttg atgtggacct gaatggaatc aaagctgggg
     9181 tggcttatat tcacacagta ggccgatcag gctgggagtt ggaaccgacc gccattttga
     9241 cgcataaccg cattctcgaa accttgaacc agtcgtatga gttgatggcg catgaagtgg
     9301 ttgtgaggaa caaggtgttt tgttcgctta ctgaggcgct gacctggctt gggctttcag
     9361 atcacagaag gcgggtggaa gagatccgcg cgcagctttt gagggagttt actttagcag
     9421 cctgagaacg gttgtgagtg ggtgaagttt caatgacatc atcccaaaaa atatagataa
     9481 cgccgatcaa agcaaagatc ggcgtttgtt ttcaaatgcg ggttttactc gaacaggggc
     9541 gccatttcaa ggcgttgact gaggtcttgc atctgtgctt cgctgggacg ggtggtgaag
     9601 cgatgggcgg catcgaggat gcgcggcagc aggttgacat cgcctgcggt gttgaggaag
     9661 acctgcgggt tgccgaagac ccaatgcatg gcaaggtcga tatcggcctg ttcttcgagc
     9721 ggacgatacc aggtggtgcg attttgctgc acatctttcc acggggaacg ggtgatgcct
     9781 ttgatggttt gcacggcgat attccgctct ttgcaaactg caagcacttt gttgaagttc
     9841 tcagcatagc gcggattctg catcatgatg tagctgtatg gcagcaatac ggaatcaaaa
     9901 tcaaattgag caagtgagcg tagatggaat tcgggtactg gcacgccatg gcctgtcaca
     9961 ccaatgaagc ggaccagccc ttcggcacgc gcttggatca cggcttcgat aacgccgcct
    10021 tcgccgaacg ctgtgtccca atccttttct tcgtgcaggg catggagttg gatcatatcc
    10081 acctggggga cgtgcaacag gtcgagtgag cggcggatct ggtcgtaggc ggcctgcttc
    10141 gtgcgctgct ccgttttggt ggcgaggaag aacggtttgc catgacgtcg aatccaatcg
    10201 cccagcactt cttcggcttt gccgtaactg gcggctgtat ccacatggtt gatgccgttt
    10261 tgcaggacga ggtccatggc ggcatccaca tctttttgcg gggtgtccca aaatgccgcc
    10321 gcgccgaaga tggtccgcgt gctttggtgg cctgtgcggc caaagggaag ggtggggatc
    10381 atgggtggct cctttttggt tttattgtga aatactgagc gggattccga aatccaaact
    10441 ctgcccgctg attctatcta caattcgggc aaagtactga ttttcacctg aactgatcga
    10501 gatgcctatg gatacatcac ctgtatgaaa tacgggcatg tctttcctga ggttgccaag
    10561 gttgatcgtt tgttgatcac gaaagcggat tgcatacaga tcgccgaccg ttgtgcaata
    10621 gatcaggaac ttttcattgg gcgagatggc gtatgagcct tttgcaaaag gtggggaatc
    10681 aaagacccaa ttggggcagc cttcgatatg aaggatgttc cctttggaag tgtcgtttgg
    10741 ggtgaccatg acgccgtaac aaggcggtgg ttccggtgtg gaggttgaca tgggttccac
    10801 tgtgacctgt gcttcttcgc ttccctgaaa ttcttcggcg acggataccg attgcaactg
    10861 gagaatgata tcgccatcgt cctcctgctt gagatttacg cttctgtata gtttgttgct
    10921 gtcgtcgaaa gcgattttga attccttccc ctcggcaagt ttgtaaacgg tggctttgtc
    10981 atccaaaatg acgatcaaga gtctggcgtc gtattcttca cgaagcgagt aaacatctgc
    11041 gccaaaagat atatcgaatg tgccaatggg agtgtgtaat tcgggggata accccagttc
    11101 gatttcccca gcggtgttga tcgaaaccct gacgggcaca atgatgggcg gagagtagcc
    11161 aaactcaacg ggttgacatg cgctgaagag gatacacatc aataacagga aacttcgtat
    11221 aaaaaccctc cgcatggcat cctccttgtt gggtttgatt atccagcctg ataggtggag
    11281 gcagttgaat aacccacctc tttctatagt ttatcgtaaa tgctcttgaa agcttgctaa
    11341 aacgccccca cccgtcctcc cccaaatacg acagaagtac cgtcgtattt gggggaggtg
    11401 cccgctaggg cggagggggt ggcggagggg gttgaacagc ccgcatcttt ccaaagctta
    11461 tcgtcaatgt ttttgaaggt ttgctcgaac aaggtgcatt tctcctctat gatgcccaag
    11521 tatacctgac ctgaaatcgt tccccgcctt gcagcagcct gaaaaaatcc gtgcgatact
    11581 tgccccagtc aaaaaatccc atgcacattc caaaatctca agcgggcaag tgtaatttcg
    11641 taatttagct gtgttatact ttcttgcgta aaggtataac aaggagcgtg gtatggtact
    11701 gacgatttca aataaaggct gggtggtaat ccctgctgaa ttgcgaaaaa aatacaacct
    11761 tcatgctggt tctgaagtgg tgatcgtaga ttatggcggc gtattggcaa tcatccctgc
    11821 agccaggtac cctgttaaac agggaagagg tttcctagct ggagcttctt ctgcctccac
    11881 tgacttgaaa aaggatcgga aactcgagaa aaagcgggat gcaaggctcg ccaatgagta
    11941 aaacaataga ctacatattg gatgccttcg cattcatggc atatctggag aatgaaccag
    12001 gagctgagag ggtggaaacg atacttcaag atgttgaagt tggaaaagcc cgcgcgtatc
    12061 tctcgatcat caatcttggt gaggtcattt acacaactga gagaaaatat ggcctttcca
    12121 aggctcagga tactttggca ttaattcaac ggatgccaat cgaaatcctg ccagcggata
    12181 accaaactgt ccttgccgct gcacatatta aggcaaatta tccactttcg tatgccgatg
    12241 cgtttgtagt gattgctgcg cagaaacttg atggcatcat tatgactggg gatccagaat
    12301 tccaagatgt tactgaactt gcaaagatcg aatggttgaa acagagaaga taatcccatg
    12361 cacattccaa aactttaccg cgaagaagac agtgaattga tcgtcaaatt tctcaagcaa
    12421 aacaactttc ccgcccttgt cacctttgat ggcgaaaagc ccattgctac gcacctgccc
    12481 gtggaaatta cccagaccga aaatgatggg ctgaccattt acagccacat gtcgaaggcc
    12541 aatccgcagt ggaaatcgtt cggtgggcag gaagtgctgt tgatctttca aggcgcgcac
    12601 acctacattt cgccgcgctg gtacaaccat gtcaatgtgc cgacgtggaa ttacatgatg
    12661 gtgcatgtct acggcaaagt gcggatggtg gaaggggacg agttgtattc cctgcttggc
    12721 cgtttggtac gtcaacacga agcggattca aattatcggc tcgaatccct gccgcaggat
    12781 tttgtaaaaa aggaaatgaa cggcactgtc ggtttcgccg tggatgtcac ccgcatggat
    12841 gcagggtata aactcagcca gaaccgcaac gacgaagacc acgccaacat catccgcgag
    12901 ttggaagggc ggggcgatgt gaattcaatg gaagtagcnn nnnnnnnnnn nnnnnnnnnn
    12961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13021 nnnnnnnnnn nnnnnnnnga caactgatta ccgccaactc ccccctcacc ccactacatt
    13081 ctcaaaatga gtcaccactg ggtttgagcc tggcttgcct tccactgcaa tcacatcgat
    13141 ctgccaatgg tcaatttcat gttcggcgca gtaatgctgg gcggcgttga gcatgtgttc
    13201 acgctttcgc gctgtgatct gtgcttcggg gaaaaaattg gcgctggagg tgagcgtctt
    13261 cacttcgacg aaaagcgtca catcaccctg tctggcgaca atgtcaattt cgccgtatgg
    13321 cgtgcggata tttctggcaa caacctcgcg tccatgtgca gcgagccact cggcggcaac
    13381 gccctcgccc catgcaccga tctcctgatt gtgcttcgtc attgcgtgat ccgctccagc
    13441 aatttgcgca ggcgcaccga ccaatgtccc ttgcgcggat gtgactcacg caccagtctt
    13501 tcataatgtt tgagcatcat gtccgtggtc cgctcgatgg cgtaggccga agatgccttt
    13561 cgcgctgagt cgctgagttg tgtacgcagg ttcgggtcaa ggcagaggcg ggtgagtttg
    13621 gcagtgtaag caggcaggtc atgagtcgca agcaggcccg tcaccccatc caccaccgta
    13681 tccccaacac ccaccgaatg aatccccatc acgggcagtc ccgcgcccat tgcttcgatc
    13741 accgaaagcg gatgcacctc tgtcaccgaa gccgtcacaa aggcatcgca catggcgaga
    13801 taggctggaa gtttttcgta ggccactcgg cccaccatgc ggactctacg ggtcagattc
    13861 atctcttcga tgagattgcg gatctcttct tcgtacggct ggatgccgct tccaataagc
    13921 agcagttgtg cgttggggat ggcctgcgca acccccgcaa atgatttaag caggaaaggc
    13981 agattctttt ccagcgcaat gcgccccgcg tacaccaaca gaatgtcatc gggtttgtat
    14041 ccaagctcag cccgtgcaag cggattggca ttctggaaat tctttaagtc cacgccgttg
    14101 ggcaccactt ccactttgct ttccacattg agttgacgca gcactttttc cattcccgct
    14161 gaaggcgcaa ccacgaggtc cactgcattg cagaaggagg gcaggtaggc ttgcaaaatt
    14221 ccctggctca tctcttcagg catgatcggc aggtaggtct gtgcatacag gtcgtagcgt
    14281 gtatggttgg tgtacacaat ggggatcttc agcagacgac agtaacgcag cgccagcctt
    14341 ccgctcaaaa acggatgatg cacatgcacc acgtccatgg tttgcagtaa ttgcttcgcc
    14401 gtccgcgagt agcgcgtgga taaataaaag ccagaatccg agagcggcag gcccttgctg
    14461 tagatcacac gcggctcatc tggctgatgc tccagatcgc caaacgtgaa gacgtacact
    14521 tcgtgccccg cctgttcgag gtacttttta ttgacctcga cataatttgt aatgccgctt
    14581 acatacggct tgtaactgtc aaccatcatt ccgattcgca tgttaatatg ctacggcatc
    14641 gaagaatgaa tgtcaaggaa ttggaagtgg ggaatgcttc gcgtgataaa atcgttttat
    14701 gagccaaatg attgcgaaat tgattctgcg aaacaaagtg tacgaagtga aggcgggtat
    14761 ggcgctggtg gatgccttga agaagaacaa cattgtcccc gagtcggtca ttgccacgcg
    14821 gaacggcgac ctgctcaccg acgacgaaat tctgcgcccc ggcgatgaga tcaaactcat
    14881 tgcggtcatt tcgggtggtt gacgatgaaa tgtaaaaaat gcggtgaaaa agccgctgtt
    14941 caaatgcgcc agcacaaact ggctttgtgc aaggatcatt acctcgaatg ggtgcctgag
    15001 caaaccgaac gcttcatcaa aaaatatgaa atgttcagcc gcgatgaaaa gatcctcgtg
    15061 gcggtttcag gcggcaagga ttcgctctcg ctgtgggata ttcttgtgcg cctcgggtat
    15121 caagccgatg gcctgtacct tggcctcggc attgacggtg gcatcaacta ctcacacgaa
    15181 tcccagcggt tgtcggagga attcgcccag cgcaacaacc tcaaactgca tgtggtggac
    15241 attgaaaagg aatacggcca atccattcct gtgctggctc aaaacagtca ccgtggctat
    15301 ggaaggccgt gtgccgtgtg cgggctcgcc aaacgccacg aaatgaatcg cgtggcgcgt
    15361 gatctcggct atgatgtcct cgccactggg cacaacctgg atgatgaagc cgccgtttta
    15421 tttggcaaca ccctgcactg ggccagtgac tatctcttgc ggcagggacc tgtcctgcct
    15481 ggttcagatg ggctggcacg caaggtcaag ccgttgtgtc gtttttacga acgcgaaatg
    15541 accgcctacg ccattgcgcg gggtatcgaa tacatttatg aagaatgtcc ttttgccgaa
    15601 gggtcgcagt ccattttcta caaagagtcg ctgaatcttt tggagaccgc acgccccggt
    15661 gccaagctga ctttctactt gagttttctc gaagcgaaaa aaagcgggaa tttgttcatc
    15721 gaaaagaacg aatcgttcaa aaatgtccac acctgcccga aatgcggtca acccaccacg
    15781 acccccgatt tttgctcatt ttgcaggatg atcgaaaaaa gcgccgccac agaagaaatg
    15841 gaataagtcc ccgcctgatt tggatggaaa gtaaagcgaa cccagtttgt gggctatctt
    15901 aatatcgtta cgatcaaacc caacacaaat aaccccatgg cagtgtaggt ggccagttgc
    15961 tcttgcggga ataacgaaaa agagggaatt tcaaaactcg agcggctgga ggttatatgt
    16021 tcaggcgctt cttttttccc cagcagggct tcacggaatt caatgacatc cttcgggcgg
    16081 tcatcagggt gtagcgacat ggcccacagg atggcttttt ccacgcggct gctgagggtt
    16141 gggttgattt ctcgaggcgg tgtcaggctg cggctattga ggaagcgttt ccgtgcttcg
    16201 gtgggtggtt cgttcgttac gaggtgatac agtgtggcgc caaacgaaaa aatatccgca
    16261 cggatatcgg tatgtgaatc atctccgccg tattgctcca gcggcgtgta cagcgcggtg
    16321 ccctgtccct gaatgatggt gatggtcact tcattgggtg cgaggatctt tacgaggcca
    16381 aaatcgacca gcttgatcaa tccgctgggg gtgatcttga ggttgctggg tttgatatca
    16441 cgatgaatga tgggcggctc ttgctggtgc aggtaattca acgcgtctga aatttgcacc
    16501 gcccaacgca atacttcatt ttcagagagg aaggccttgt tgcggcgcgc atccagcatg
    16561 cgttcgcgca ggtctttgcc gggcacataa tccatcacaa ggtaatcgcg cgggccttcg
    16621 gagaagaaat ctgaaacctt gggcaggttc gggtggtcaa ggcgtgcaag aacagatgcc
    16681 tcacgaaaga attgttcgcg ggcttgttct agaaattgtg tagggagggt gcggttgtgt
    16741 tcaacttcct tgattgcaca caacctgcct tcaagacgat tgtcctcggc aagataaata
    16801 ctgcctgtac cgccctgtcc gatctgttca cggatcatgt agcggtcacg cagaacctcc
    16861 ccattttgaa gcgactgggg caaatcttct cctttgtggg aagcgtgtgt ctctgggttt
    16921 atgctttggt catggcagtg tgatggtttc ggcgtatatc acctgcgtat atcaagacca
    16981 taaaaaaaga aaagcagtat ccacagaatt acacttttct gctatattat aaacgtcttt
    17041 gttgttatat agtactgtgc agacatggca atccaggact ttcccaaagt cggaaaccat
    17101 taaaataaat acaactccca aatgatacga atgactttac aagtgttcgt gttgtggatg
    17161 ccgcacagca aattttattt ttgcaatatg cctagttgag gtcaaaatca tggatcatga
    17221 agatgaattt cccattctgg tggcacagga aggcccgctg aaaggacaac gctggtcgtt
    17281 gagccgcgcc ttgatggttg ggcgcgaccc cacctgcgat atcaacgtgc aggaccgcca
    17341 ggtctcgcgt tttcacgcgc gcatcacgcc caccacagaa ggtgtgaccg ttgaagacct
    17401 tggcagcaaa aatggcacca atcataacgg cgttgaatta accggccccg tcatgctgca
    17461 agatggtgac ctgcttggga tcgccattgc ccagcaattt ctcttcctca cttcagatgc
    17521 caccatgccc ctcgccgaaa gcggagcccg ccccgggcgc ctgatgatgg acaacaaatc
    17581 gcgccgcgtg tgggtgaacc agcaacaggt gacgccgccg ctttcagcgc agcaattcaa
    17641 gttgttgtgg cttttgtatg aaaaccaggg acaggtcatc agccgcactg accttgtcgc
    17701 cactgtgtgg ggcgaagacc aaatggcggg ggtttccgat caggcgttgg atgcgctcat
    17761 ccgtcgtctg cgcgaccgcc ttgcttcgct cgacccaact catcaatata tcgattccgt
    17821 acgcggtcat ggcgtacgat tagataaccc tccctcaggt ggataaatca tcatggacgt
    17881 tcaatcagaa aaactacttg ttcgtattct ctccacaacc cgcattgcag ccctcggcac
    17941 gatccgcgat gaggagccgc gtgtttcgat ggtggcctac gtggccgccc acgattactc
    18001 ggcgttttat gtacatgtca gccgtcttgc ccagcataca gtggatatgc aaaagaataa
    18061 gcaagtcagc ctgcttattt ccgaaaccga tgacggccgt gccgacccgc agacactggc
    18121 gcgtttatcc atccgcgctt cggcggaatt tatggaaaac ggcgaacccg gttacgcccc
    18181 cgtgaagaaa atgtacatcg aagccttccc cgaatcagaa ccgctgttca agatggcaga
    18241 ttttggtttg tggcgcatca agcccaaggg cgcacgatac gtcgcagggc ttgcgaaggc
    18301 tttcaatctc acccctgaaa cgcttcaaaa agtcgctgca aaataactcc ccaatcccgc
    18361 acatgttgcg ggaattttta ttgcatcagg agtccaaatg gaacaactaa aattacttgc
    18421 cgtttttgca caccccgatg acgaatcgat gggcatgggc ggcacactgg caaagtattc
    18481 tgccgaagga gtggatacac accttgtttg cgcttcacgc ggcgaggtgg gctggttcgg
    18541 tcccgaagag cagaatccag gtccagaaag actcggccaa ctccgtgaaa aggaattatc
    18601 caacgctgtc catgaactcg gaatgaagag tctgcacttt cttaattaca ttgatggcag
    18661 tgtggacaag gccgaccatg ccgaagccat tggccgcatc gtcacacaca tccgcagaat
    18721 caaaccgcag gtcatggtga cctttcctcc cgatggaaat tacggccatc ccgatcatat
    18781 cgccgttgga caattcacct ccgccgccat cgtttgtgcc gcagatgcaa gctatgttga
    18841 tgcagagaat ctgtcgcctc atcgggtgtc gaaactgtat tacatggtgg atggcgaaaa
    18901 ctttatcaat ctgatcgcgc ctttcatggg cgatatgacc ttccctgttg atgaccagat
    18961 ccgcggcgag atcgcctgga aggaatggat gatcaccacg cgcatcgaaa tgtcagcgca
    19021 ttgcaatgcg gcatggaagg ccattcaatg ccatcagagt caattgccta cacttggcgc
    19081 gctcgcagaa atggacgcgg atgccgcggc agcggtcctt gccatgcagg gtgcgtttta
    19141 tcgggtattc agtttggtca acggcggcag gggattggag acggatttgt ttgaagggtt
    19201 gagatagttt gcttgaacaa gcaaagaaga aagaggaaag ctgttctttc ctctttcttc
    19261 tttttattta acttaattac ttcacgatct tccatgccgc aggcagagcc acggcagccg
    19321 ccagaagttt gatcacatct ccgatcaaaa acgggaccat gcccaatgcg accgctttgc
    19381 tgaaacttcc cagcagaatg gacagccagg tgatgccaaa tgcgtagatg atggttgtgc
    19441 ccaccaaaaa cggaatgaag gaagtgcgcg catttcgttc caggccgcgc tctgcaagca
    19501 ggccaatgat gtacgcagca gcgatgaagc cgattaaata tccgcctgtc gcgccaacga
    19561 cgacgcttaa tccatgggcg ccgccagcaa agaagggcag gccaacgatt ccttcggcaa
    19621 gatacagagc cagcgacatg gcaccgcgcc ttgatcccag ggctgcgccg atgatcagca
    19681 cgccaaaggt ctggccggtg atcggcacag gcgtaaatgg cagtggaatt tcaacctgtg
    19741 ccagcgccgc cacgaacagg gaaccgaaaa cgataagcag caggtcgcgc agccatgtgg
    19801 ccgtgcgtgg aaaataccga atggaaagtg tgggtgcaag agttgtcatg agtgtttttt
    19861 cctttttata aattatagtg tacttgatac attgcaacgg tcatgttttc tgccatcttt
    19921 atcaggtaaa acgacacata ctttcatttt gcttttgtta cctttgttca gggttgaaat
    19981 caagtaaaat aacaaaagtt gtttccattc tccttcccct gttttttacc tctattctcg
    20041 aatgaaacat ttctttgatc aggcacaaag acttcccgaa gatacctctc agaacgcagt
    20101 ggagagtttt tttcgcgggc aaaatgccag cggcttgatc gaaaaacaat tacattctgg
    20161 taagtctgta attcttcttt ttgcaaatgg ttcgcccagt gcagcctatc agctcgaaaa
    20221 cggacttgga accttcatta cacatggcga attctctgct gtgcaaaatg aaattgccaa
    20281 tgtccgcgcc ctgcaattgc ccgatgtggc tggacgcctg acgctgttgg cgcttgagtc
    20341 tcagatcggc gatgagcttg ccatgcccga ttcggtgatc tggcaggccc aacttgaaaa
    20401 atggaagcag gcacgctgga gtggtttggt ggaagtagaa tccaatgacc tgcaagggct
    20461 ggctttgttt tggcagggtg aattgcaagc gtctgatgta attttttcaa cagccagcgg
    20521 attcgtaaca gaactcccga aggcggggat ttttccatgg aaagtcacaa cctatttgcc
    20581 tgtacctact gcccagggtt atcaatgtgc gatcctgcgc ctcggggctg tgcattggag
    20641 ttttcggatt ctcagccgct atcaagagat ggtcggccaa aaactactgc aaaccatgga
    20701 ccgtgaattg aatcgccaga tccagccctg gcgctggaaa attgccttaa ccgacagcaa
    20761 catgctggat acgcacttct tcccgtactt aatggatgcc gctcatgcct accgcgccct
    20821 tttcatggcg atgggcgcgc aaatgaattt tgtcattggt agtaacctca ccctgcgtct
    20881 gttgaacgaa acctttgaac agattcaccc cgatgaacgg gcgctattgc aatctcagcg
    20941 cctgatccca gctgcatttt cggaatgacc tcatgagcga atctaccaac caaactggcg
    21001 gcaaccgcct ttcacccaaa acgatcctcc gtctttacat ggcggtctct tttttcatcc
    21061 tgctcctcat cctgtggacc tactatgccg agccgcttac cgaatcagtt tcagcttact
    21121 ggaacgacaa gcttcttgat acgctaatct taattcccgc gttgggcgcc gccatcgcag
    21181 gtgtgcttgt tgtccgtcaa tttgatgcag gcgaacaacc ccatcgaatc tggcaggcct
    21241 ttaccatcgg tctgtggttt tgggtggcag gtgagttgag cgggtttgtg tacgacgcga
    21301 tctacataga tcttccttat cccgaattta gtctcattga tgtctgctgg ctggcggggt
    21361 atttttatct cggattgtcc ctttattatc aggttcgtct ggtgtacagc gcccaaaaac
    21421 gcaaaggacg gttgttgtat ctggcattgg tgggcctggc attcctgatt gccgccctgc
    21481 ttacccaatt agccgccaaa gccgggctgg gaggagaatc cgcgtggatc attctattcg
    21541 tctccatttt gtatcccgtt ttcgaccttg ccgaaggctc ggctgcgatc tggctttccc
    21601 tccttttcgg gcgcggacaa tggagccgtc cgtggtgggg gcttattctc tttgcgctgg
    21661 ccgatagcgt ggataccttt tattggcttg gcggttatga tttcatcgcc cctgttgttc
    21721 aatcagttct ggatgtcatt tccgtggcag cgtacccagc cagttatatg gttgccgggc
    21781 tggccttgtt gtcgaattat ttcatcctgc aatacggtgt cgaaagcggc cttttgcaga
    21841 cccaaaagag gaagtaacgt ttgtcacttg acgtctcaat ggcaggggct aaactagtta
    21901 tataaaaagc tgtgcgcgat gggaggcgat attttggggc aattgtgtct gccaatccat
    21961 ctattctcta gtttggtaaa gtatcccaac tttcggaagg cacaatcatg agcttgaaaa
    22021 attctccaca gaaagtaaac attctaaagg gcagggattc ggctgagttt cggaaaatgt
    22081 tcggtaacca ccccacgcct gtctggattc atgatcgcga gtcgtttgcc atcctggctg
    22141 taaatcaagc cctaagttcc ctgctgggat acgaaccaac cgaactggag gggatctcgc
    22201 ttgaaaatat ctttcctgtg gaagaagtct cgtctcttta cgccgacctg aaactgcctc
    22261 gacaaattgg gcattctcca cgcgaatggc atctccgcag caagagcgga caattgatca
    22321 gcatcgacca aactttcagc agtttcattt atgaaggtcg tgaagctgcc ctggtcattg
    22381 tccacctaag aaccgaccag acccttgaaa gccgtattct tggctcacgt ctgcgaatca
    22441 gcgaattcgc tcactcacat tctctcgatg acctgctcca aaaaacattg gatgaagccg
    22501 aattgttgac ccaaagcaat atcggctttt ttcattttgt tgaagcagat caaaaaaccc
    22561 tttcacttca aaactggtcg accaacacct tgaagaacat gtgtactgcc gagggaaaat
    22621 ggtctcacta ctctattgat gaagcaggcg tatgggtgga ctgtgttcga tttggcggcc
    22681 ctgtcatcta taacgattac ccagggcttg aacatcgcaa aggccttcca ccggggcatg
    22741 ctcccatctc acgattgtta tccgtcccag taacacgcgg cgggagcgtt gtagcaattt
    22801 tgggggtggg aaataaagtg gaggaatata atgaatcaga tatcgcatcg gtgtatttac
    22861 ttgccgacct ggcgtgggat atcacagtag ggaagcgcac tgagcatggt ttgcaccaga
    22921 gcgaagaaag actcaggctg ttgttcgata aaatctccaa tatcgctgtg cagggatatg
    22981 cccccgatgg aactgtacgg ttgtggaatc aagccagcga gaagatttac ggttacacaa
    23041 aagatgaagc gcttggaagg aacctgctcg acctgatcat ccctgaagaa atgcgggccg
    23101 atgtcgaagc ctctgtcaaa aaaatgatag ggaccatgca gcccattcca tctgaagaac
    23161 tgtcgctaaa gcgcaaagac ggctcgcggg tttcagtcta ttccaaccac acggtcgttg
    23221 agatttcagg tgttggcagg gagttgtatt gtattgacat tgacctctct gaaagaaaat
    23281 catttgaaga ggccctgcgt gaaagcgagg aaaaatatcg ccagctcttt gaagccgaat
    23341 ccgatgcgat tttcctgatc gataacgaaa ccgggcagat cttcgaagtg aattcggcag
    23401 cgacaaaaat atacggctat acccgtgaag aactgattcg catgcgtaac gtggatcttt
    23461 cggctgaacc cgataacacc cgcaaagcca tgcagtccaa aatggtgaat attcctgtcc
    23521 gttggcaccg caaaaagaac ggtgttgtgt tcccggtgga gatcagtgcc agccatttga
    23581 tgtggaaagg acgcccaatc cacatcgcag ccatccgtga tattacagaa cgaaaacagg
    23641 ccgaagatgc cttgcacgaa tcggaagccc agtaccgcct catgttccaa aataatccac
    23701 accccatgtg ggtctacgat ctgaacaccc tcaaattcct tgaggtcaac gatgctgcca
    23761 tttcacatta tgggtattcg cgtgatgaat tcctgcgaat gacgattttg gaggtgcaat
    23821 ctgtggatag tctggccatg ctgcgggcac ctttggtcga tcccttcctg caggattacc
    23881 gttctggagt ttggcagcat cgaaaaaaga acggaatgtt gatcgaagtt gagatcaccc
    23941 agcatgatac attgttcaac ggcaatgcag cgcggttgat ccttgcgaac gatatcaccg
    24001 agcgcaagca ggctgagaac gccttgcgtg aaaaagagtt gcgccatcgt atcgtggccg
    24061 ataacacagc agattgggag ttctggcgtg cgcctgatgg aaaattcctg tactcttccc
    24121 cctcttgtag agaagtcacc ggacatacca tggaggaatt catttctgac gacagattgc
    24181 ttgaacgtat catccaccct gatgaccttg ccacattcaa taaccacaga cattttgaat
    24241 tgacggcaaa gatgtccagt gaggtcgaat tccgcattgt gctacccgat ggctgggtac
    24301 actggatcgg tcatgtctgc cagcctgtgt ttgatgatca aaacagttac ctcggcatcc
    24361 gcggaagcaa tcgcaacatc actgcccgta aacaagctga agaagacctg catcggacaa
    24421 aaaagtctct cgaagaggtc aatagcgtgt tgctggctgc cattgagcgc gagcagcgca
    24481 tctcccgcac cgatagcctg actgaaacat acaaccgccg ttattttttt gagatcgcca
    24541 cgcatgaatg ttccgtggcg gaaaggtacc ggcagccgct ctccctcatc atgttcgata
    24601 ttgataactt caaaaaattc aatgacattt atgggcatca ggtaggggat gaggtcttga
    24661 ggcgcatcgc ccaggttgcc cgtgaacaat tgcggtcagc agatatcctt gcgcgttatg
    24721 gcggcgaaga atttactgta ctgcttccaa aaagcaatac gcatgaggct ctgatcgtgg
    24781 cagaaagaat ccgcgaaaag gttgccgcct gtaaactgga tactggaaac ggagtggttg
    24841 gcatcaccat cagcattggg gtggccgaat acttccctga ccaacaaaca ctcgacgacc
    24901 tcattcgaaa ttccgacaag gctttataca cagcaaagga gaagggtcgg aatcaggttt
    24961 ctgtttatgc aacaggcaat acggtataat cttccaaaca aattcaatac attccagttt
    25021 gaagaattgt ggcagtgaac gccctctttt gcagtatggg gttatacaat atggcaccca
    25081 catgaaagaa aatcaggatg gtgtcaacga aaaccgtacc acacacccgc gtaaaagcga
    25141 aaataaaaaa agatgacgaa aggctgcgtc ttgctctgag gctgtcgcca tccccaatgt
    25201 ggatttttga cttagagtca ttattgttcc ttgcagccaa tcgacctgcc caacttcagc
    25261 tggaatacag cctgcctgcc ctgaagcgaa tgaagctcag tgatatcatc ccagccgatg
    25321 aacacccgaa tctttattta accttgaaac aggtcggccg ttttgggcat ttccccggcc
    25381 gttggcatct cctccccaaa agcggacaaa cgatcagcgt ctctgtgaat tcaagccttg
    25441 taaaattcaa tgggcggaac gcagtccttg tctcagcttt cgcatcacct ttcgaaggtc
    25501 ttttaacaga aaattgttcg cgctattttg cccttgttgc gaacaggatt tcaagcgcgg
    25561 tcatttccac cgattctgct ttgcgtatca tccagtggaa caaggccgct gaagacctct
    25621 atggactgac agaaaccgaa gccatggggt gtttaataga tgaggtttgt tgcactgagt
    25681 tcggcgaaga ccaacaatcc gaagctcgtg ccagccttcg cacgaaaaaa cgctggcagg
    25741 gcgaattgaa acagcatcat cgcaatggag cagaattgtg ggtctccact tctgtgacct
    25801 tgctggaaga cgaaaacggg aattttcttg gcgggatcac cattaatcac gacatcaccg
    25861 aaagaaaaaa agtggaggat gatctgcggc atacaaaaca ggtcatggag gaaatcaacc
    25921 agatcttgca gcatgccttt gagcgcgagc agcttgcctc ccgtaccgat gccctgactg
    25981 gtatttttaa tcgccgctat ttttttgaat tcatcgaata tgaggttgcg ttatccaaac
    26041 gataccagca gcccttttcc ctcatcatgt tcgatgtgga ccatctcaaa cttgtgaatg
    26101 atacgacggg gcaccaagct ggcgatgaag tcctgaaaca aattgtcctt ctagtacaga
    26161 aggaactgcg cagtgttgat attctggctc gttatggtgg tgacgaattc gtcattttgc
    26221 tgccaaacag cagcgtgcca gatgcgctga ttctggcaga gagaatccgc agccagattt
    26281 ttgccgccaa tttacgggtc gctggatcga gtttgcaggt gactgtcagt attggagcgg
    26341 ctgatttcaa tccctcggtg tcgagttcgg ctgaattgat cagccatgct gatgaggctt
    26401 tatattctgc aaaacaagcg gggcggaatt gcatccttgc ctattcggca aaaatttaat
    26461 acggcttact cattttgtat tccagcgact gtttcaccac gggccattcc aaattaataa
    26521 tggaaaatac aacggtgtcg cggtacaccc cgtttgcaga tttggtgtga ttcctcagca
    26581 caccatcctg ttttgccccc agccgcgcaa tcgccgcgcg ggatttgtga ttgtgccaat
    26641 gagtgcggaa ttccaccgca atggcatcca gtttttcaaa ggcgtgagtg aggagcagat
    26701 acttgcactc ggtgttgacc gccgaacgct gataactttc tgcataccag gtgtagccga
    26761 tctccaaacg ctggttgacc tcgtccacgt tgcagtagcg ggtgctgcca atgatcttgt
    26821 tcgtcgcctt ctcgcggaca acgaacgcca tccatcccgc attctcgcgc aactccaacg
    26881 ccgagcggat gtaggtctct gccgtttctg gcgatggaat ggaagtgaac caaagtttcc
    26941 aaagttcccc atccagtact gcttctttga ttccatccaa atgctcaaga gaaagaggct
    27001 ctagaacaac atgagctccc gtgagtgtaa caggttgaat ccagcgagtt ttcattgatg
    27061 atccttttat ttcacagact tgtgtaattc tatcacccag ccatccatgg tgctcctgct
    27121 ataattccca cattatgaac tggcacaaaa ccaccatcga aatcgccaca tcgggcaagg
    27181 gactgcgaga catcactcca gccatccgcg cgcagttgaa agagtggcaa gtgcaggaag
    27241 gcatgtgctt tttgttcttg caacacacca gtgcctctct catcatcagc gaaaattggg
    27301 acccaaccgc ccgtgccgat ctggagactt tcatggagcg cctcgtcccc gaacaggaca
    27361 gttggtacac ccacgatctg gaaggccccg atgatgcgac ctctcacatc cgctccatgt
    27421 tgacggacgc cagcctcacc atcccagtgg atgaaggtga cctttcgctg ggcacctggc
    27481 agggaattta catctttgaa caccgcgccc gtccgcacag gcggaagttg ttaatgcgtg
    27541 tgctgtcggt tgaataacag aaacggcttg ctaaaaacaa gccgtttttt tcttattctt
    27601 cttcaatata aaagcggatt ttgttcaagg tcgtccactc tgtggaacca tcatcaaatt
    27661 cgacctcgac ctgctcacca tttgcattca gtacggttac gtcgtagtac tgattgtctt
    27721 ttgctgattt gcactccacc tcttcaccag ccgcggtggc atattcttcg aggtagtccg
    27781 cctgtgtcca ctcttcttca cccgtgtcaa agcggatata aatatcctct tcttccattt
    27841 ccagaatggt ggcagggtaa aaatagtcat catcatccca atacgcaaat acacgatcgc
    27901 ctacgtccca atattccatt tttctttttc ctttttgttg gttgttttat cgaaaagatt
    27961 ataacaaacg aaattaaa
//
LOCUS       DAOQLC010000055        27432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_574_length_27432_cov_22.847390, whole genome shotgun sequence.
ACCESSION   DAOQLC010000055 DAOQLC010000000
VERSION     DAOQLC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_574_length_27432_cov_22.847390"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..548)
                     /locus_tag="PL000_11155"
     CDS             complement(177..548)
                     /locus_tag="PL000_11155"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC89502.1"
                     /translation="MQSTAKNVTEYLKESPSERKAALTQLRRLCRASLKGFQESMMYG
                     MPVYTRNGEAEVGFASQKNFIALYILKTDVMDAHRDLLKVKGISLGKGCIRYSKPEKI
                     DFKVVEMMLKATQESTGEVCD"
     gene            complement(695..1054)
                     /locus_tag="PL000_11160"
     CDS             complement(695..1054)
                     /locus_tag="PL000_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89503.1"
                     /translation="MGVGYILVNYSKKELISFNHIGANKARELAGNPVSAAMVTWYML
                     QHTGDTVAFLSDTYGEWCFPEGTRDEISDYRDATDEVVKSLIEVEILIDLGKTWVDSD
                     EPDKIYIRALENVWMKF"
     gene            1405..2292
                     /locus_tag="PL000_11165"
     CDS             1405..2292
                     /locus_tag="PL000_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89504.1"
                     /translation="MRLKADLTLLVVSIIWGSAFVAQRVAGQQGSVYLFNGARYVLAA
                     LVVLPFVKRVNLTSMPHDQYKWMFIAGFFLFAGSALQQAGMVYTTAGNAGFITALYVV
                     LVPFALFFFWREKPHWMSVVAVVLAGVGAFLLSTGGRFEVQKGDALEVVGALFWTFHV
                     IIIGKFAAKFEAMSFSVGQLLVCGVLNLGVGLVSESMMTFNWQLAFAIGYTAFFSLGL
                     CYTLQVWAQQHTPPADAALILSLESVFAVLSGWLLLDERLAVIQIFGAVLIFIAVVLS
                     QFKEWTSGKIDHDHLVEGR"
     gene            2297..2887
                     /locus_tag="PL000_11170"
     CDS             2297..2887
                     /locus_tag="PL000_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89505.1"
                     /translation="MTEERNLIEELDLSFHPLTQKLWHDFEMLFGRRGACGGCWCMHW
                     KLRGKEFTESAGDTARQMQKSIVDSKTVPGLLAYSEGYPIGWIAVEPRSAYPKLAHSR
                     TLKPVDDQEVWSITCFFIEKKHRHKGITVELLKAAVDYVKGRGGKIVEGYPVDAKQEA
                     PAPFVFTGTASAFHHAGFVEVARNSPTRPIFRFVIE"
     gene            2950..4209
                     /locus_tag="PL000_11175"
     CDS             2950..4209
                     /locus_tag="PL000_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89506.1"
                     /translation="MSVTFQPPSKSFTARDYQFIALVSIVVVAISIGLVMLNLTLKDG
                     GDFYVHWFAVRVFQNDRLDPYSGEVPARVQQQVYGGPAAQGDEPYILDTPFHILLLYF
                     PLTLLSDPLLARAIFTLILQLALFALAFISLRLTEWEVPIIFYVLFPLLAIFNFYTFQ
                     AIYEASPVLLLGLTYAEILIALRADLDEVAGALAAVSLYYWEVGAPFLVLVALRVYYE
                     KRTRVFAGFAMASLILLVLSFMAYPNWFIPFLRATVNNIKADFGFNVHSIFIRIWPAF
                     GGTFAWMFIGLLVVALGYEWSAARSGDFRRFYWAACLSIAAAPLLGFRTEMENLAVLI
                     IPLALIFAIVHDRWYKIGNFLTILLMLLVGTLPWAVSLFLVPRFGIKAEEGLFLFLPL
                     FTFIGLYWIRWWAIRPPRILSDIASRL"
     gene            4225..5784
                     /locus_tag="PL000_11180"
     CDS             4225..5784
                     /locus_tag="PL000_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89507.1"
                     /translation="MNSRTYFFLFLFGLAVPVFIAQFQPIPGYLDSDYYYAGGIQLAT
                     GKGFTEPYLWNFLDGTTSLPHPSHSYWMPLASIVSALGMWLTGQTTFASARLFFFLIA
                     ALVPPLTAALAYAFSQKNELAIASGMLAIFSVYNAPFVGVTDNFGLFMLFGGLYFIIA
                     TQLMQNPDRMRNWLFLGLLSGLMAFSRSDGLLWLPLTFLFALWRARKISSFIPHPSSL
                     LLILLGFLAIMSPWYLRNLNLYGTPMAPGGSRALWLENYDQTFIYPPTLLTKESFLAQ
                     GWEKILDDRLWALNMNLQSGFAAHGGIILFPFIVAGIIYYRKDDRVKLAALAWLILFL
                     VMTFLFPFAGARGAFFHAGAALQPIWWTLAPLGLESILVSLRKRGWGNDQNRFVFRSA
                     LVMIAMILTVYVVYLRVFTLGWGEIDADYKAVDQFLVEHGVGEEDIVMARNAPGYYLL
                     TGRSAISIPYGGENALLEAAKQFDAYYLVLEPEAALDPIKDLFQNPNSRNRFVYLGDV
                     DGARIYKLETK"
     gene            5750..7243
                     /locus_tag="PL000_11185"
     CDS             5750..7243
                     /locus_tag="PL000_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89508.1"
                     /translation="MVRVFTNSKLNKISNRDLLMLAAAVVVVTSIYLLASYFNYGIGF
                     PLDDSWIHQTYARNLAQRGEWAFRPGVPSAGSTAPLWSALLALGFLFSLSPYIWTYLL
                     GAFTLFALTVVCEWAVRTLIDSYRPHLPWVGIFFAFEWHFAWAAMSGMETLLHGLIVT
                     TVLVLLMTNTRRYLTLGLLTGLSAWVRPDGLTLLGPVLIMVLIHEQDTRPRLIALLRC
                     LIGFGSLFVPYLLFNLAIGGTPMPNTFYAKQAEYASWQTISIFSRLGQMFLQLLVGPS
                     LVLIPGVMGWLVKSIKQKMWGSLAALVWCAGYFGLYISRLPVYQHGRYIMPAMPIFFL
                     FGLLAFADFEKSRLFERYHWIGQTIWRASLVMLSVAFIFLGAQSYSQDVALIESEMVV
                     TAKWVAANLPPDALIAAHDIGALGFFDTHALIDLAGLISPEVIPFIRDEPKLANYLNQ
                     QGADYLIAFPGFYPAMTGSLEKVFVTNSVVTLKYDEENMAVYLWKTP"
     gene            7300..8001
                     /locus_tag="PL000_11190"
     CDS             7300..8001
                     /locus_tag="PL000_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89509.1"
                     /translation="MNQITLLIVDDHPLFRQGVVDALSLEPDMRIIAQSAAGDEALEL
                     IRTKKPTIAVMDVNLPGMNGQQITHQASQDRLPTRIILMTGYDDVEQAIHAALAGAHG
                     YCSKDIEPQSLSRIIREVAEGKYVFENNVFTRRELELWVEDRIEGARRSYSEPGTPFH
                     PLSDREMEVLACVVRGMSNKEIATLLGISHQTVKNHVTAILRKFGVEDRTQAVVYALK
                     RGWVTLKDTNIHPQE"
     gene            8005..9018
                     /locus_tag="PL000_11195"
     CDS             8005..9018
                     /locus_tag="PL000_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC89510.1"
                     /translation="MTSTEPMDFQSELDETQRALREVTLMIEQSQGELGKITQRNTAI
                     TSHLQQLQKQGTTVDDLKMSYDSALDVQQRLFVMRGQLEKLQNDKSHLEKYKTVLENI
                     VSGAASGGDAAGATPAAGGKSQMAGIEMIVNAQEAERQRLSRQMHDGPAQALSNFILQ
                     TEIAMRLLDVDPAQAKDELGNLKTSAMGTFQRVRSFIFELRPMMLDDLGLVPTLRKYA
                     DAFKEQASLDVSVTVTGTERRLEPYLEVMIFRAVQELLGNASRHSQATLIKVQIDLGN
                     DTIRVSVEDNGKGFSPEILKESSNLGLKLIRERAEMLGGSFEVDSAAGSGTRIAFSVP
                     AKV"
     gene            9292..10374
                     /locus_tag="PL000_11200"
     CDS             9292..10374
                     /locus_tag="PL000_11200"
                     /inference="COORDINATES: protein motif:HMM:NF020250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNC89511.1"
                     /translation="MRRGRTLIFVVLILIIGLAVAVAAYFQFLRPAATPTQPVTTVDV
                     YMAGQPIAQGSEITPELLTTIKIAPENLVSVMYTVEMQSQLIGKVAKYPLDQGVIITQ
                     AMVSDATSAVPISGPQWAALIPTGMTAISIPADRLSLASYGVNNGAHVNVNACFLFVD
                     IDPSFQSALPNQTGQLTGTGFTPEGLPVLSLGVSASGSSQGRLELDPSIQQPYYLMPS
                     ERQRPRMVCQMLLQDVVVMKVGDFPSDPAASLSAPAPVATDPETQQAAPVPPDVVTLI
                     VLPQDSVTLSYLIHTDAELTLTLRNPTDQLRQATEASTLQFLLSQYNIPVPAKLPYSL
                     EPYTVSFCNLSLGTSAICSQTAAPPQ"
     gene            10507..11748
                     /locus_tag="PL000_11205"
     CDS             10507..11748
                     /locus_tag="PL000_11205"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89512.1"
                     /translation="MAADKIRILIVDDIAETRENIRKLLQFESDVDVVGVARSGKEGI
                     QLAQELDPDVVLMDINMPDIDGITATEQVRKKSPHVQVVILSVQNDQSYMRRAMLAGA
                     RDFLNKPPLGDELISAIRRAGEMAHTERAKSAQQSFVGAAVAGSGGAAMNFAPVHKGK
                     IITLYSPKGGTGCTTLAVNLAIALNNEDTRTSLVDANLQFGDVAIFVNEPGKNAIIEL
                     APRVDELEPEVVDDILINHGASGVRILTAPQRPEYAEKISAEQFTKVIQYLQRMYAYV
                     VVDTSHILSDVVLSIFDVSDVIVLVTTQEIPAIKNARLFLDLLQTMGINKNRVIFTMN
                     RYDKRISITPERVSENLKHEVSVTIPLDEKIAITAVNRGVPFMLDNKSQPIGRGIFSL
                     AEAVRSRLTTLESENDMSDRR"
     gene            11760..13241
                     /locus_tag="PL000_11210"
     CDS             11760..13241
                     /locus_tag="PL000_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNC89513.1"
                     /translation="MSLLRRIEQGQQGGGQQQGNGNTSGTPSPTPPAKQPGSGQGGEG
                     SGGGGDTSRLSSLQARRVSAPITSPQAGSYFDLKTRVQNKLLAEIDPSMDVSRTDDVK
                     RTIQGLFEQILTEENIVLSRPERARLFEQIAAEILGLGPLQALLEDDTITEIMVNGPK
                     NIYIERKGKLHRVPVTFESNEHVMRIIDRIVAPLGRRIDESSPYVDARLQDGSRVNAV
                     IPPISLIGPVLTIRKFSKNPISIEQMVQFGSISHEAVQFLKACVEARLNIVISGGTGS
                     GKTTLLNVLSSFIPGDERILTIENAAELQLRQEHVVTLESRPPNIEGRGEITIRDLVI
                     NALRMRPERIIVGECRGGETLDMLQAMNTGHDGSMTTAHANSPRDAIARIETMCLMAG
                     MDLPVRAIREQIAGAVDVICQQARMRDGSRKVIAITEVSGMEGDVITMTDIFTFEQTG
                     MENGRIIGRTRPTGLRPKFMDKIEAAGIHLPPSIFGIGERRRY"
     gene            13267..14220
                     /locus_tag="PL000_11215"
     CDS             13267..14220
                     /locus_tag="PL000_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC89514.1"
                     /translation="MLNIIIIGGVVLIILLIIGVVVSSNSERAVVEQRLAQYLDDDKQ
                     DIDSAAQNSVLTEWVSKRVEKTSFGDRIARDLARADLKFKAGEYFILIIVSILLGGLV
                     IWFLGNQHWASAVIGAVAGAFAPGIYVRRQQKQRLQKFNDQLSDMLNLMVNGLRAGYS
                     TMQAMEAVSKELPSPINDEFRRVVQEMQIGIPMETALDNLLRRIPSDDLDFVVTAINV
                     QREVGGNLSEILDTISFTIRERVRIKGEVRVLTAQVRTSGSVLSLIPFFLVLILWFLN
                     PEYILAVTAGGPIITAAIICVVLGLIFTGYFIMMKIADIEV"
     gene            14223..15182
                     /locus_tag="PL000_11220"
     CDS             14223..15182
                     /locus_tag="PL000_11220"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC89515.1"
                     /translation="MGTIVGIILGIVVVGGVIALIVVGMRARQGGGDESDPILSRLAE
                     ATQRGETVSLEDIELQQPFIQRVIVPMIEKVGEVSSRFTPQKLLQETTLKLELAGNPG
                     RIDAATFLATRFFGAGIFGGGLLLIALASQTWPVGRIILVVGFFTVIGFFFPQLWLQS
                     RINSRQAEVRKALPDALDLLTICVEAGLGFDAAMSKVAEKWENELSLMFGRCIREVQL
                     GKTQREALRDMADRLGLPELTSFVAAVIQSQILGVSLAKVLRIQSDQLRVKRRQFAEE
                     LAHKAPVKMIIPMALLTFPSIMIILMAPAAFQIAGAFGPFLGQ"
     gene            complement(15287..15526)
                     /locus_tag="PL000_11225"
     CDS             complement(15287..15526)
                     /locus_tag="PL000_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89516.1"
                     /translation="MQRIAHRVFEYGPCTQGMKMRPVRKAGVTDACAFPGKAAYFTQI
                     SIQDLHPLLTFRTPTGASFPTSATLRWIQEVQTSP"
     gene            15578..15982
                     /locus_tag="PL000_11230"
     CDS             15578..15982
                     /locus_tag="PL000_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HNC89517.1"
                     /translation="MSDFVRTLNWQADMIVPIPLGKQRYRERGYNQVAMIAKPLALSL
                     RMEFAPQTLSRRKETRSQVGLTKEERRVNVHEAFQAGTGVKGKNVIVMDDVSTTGSTL
                     SSSAEALFRSGARNVFALTVARALPHHGLRHA"
     gene            16025..16588
                     /gene="raiA"
                     /locus_tag="PL000_11235"
     CDS             16025..16588
                     /gene="raiA"
                     /locus_tag="PL000_11235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNC89518.1"
                     /translation="MSNKVEVQTRNIRLTEKIEEYVNKKAGNLDRYLPTIDSARVELA
                     HHQAARNATDRNVAQITVYGKGFTLRTEERADEALAAFDRAIDNMQTQIERYKGKHQH
                     GRGDGRSAAEAAEPIIDDETGELSPLVARKKKFVLYPMNEEEAVVQMRNLGHDNFFIF
                     YNAETSKINVLYRRRNGSYGLIEPELG"
     gene            16681..17304
                     /gene="folE"
                     /locus_tag="PL000_11240"
     CDS             16681..17304
                     /gene="folE"
                     /locus_tag="PL000_11240"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNC89519.1"
                     /translation="MEFDDVNESDVEGSAIDNAAIETAVTQILNAVGEDPQREGLQFT
                     PRRVARMYHELLGGYKMDPVAIINGALFNVKYDEMVLVRDIEFYSLCEHHMLPFMGRV
                     HVAYIPDGKVLGLSKIPRIVEMYSRRLQVQERMTRQIADFLRDLLKPQGVAVVVEAMH
                     LCSMMRGVKKHDARMTTSAMHGAFRANLATRQEFLANISRGSAPLQL"
     gene            complement(17414..18172)
                     /locus_tag="PL000_11245"
     CDS             complement(17414..18172)
                     /locus_tag="PL000_11245"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC89520.1"
                     /translation="MIELFRNLFSPPRHMILLVAAAWFGLSFAEKRAERHGIQKDDLN
                     NVIFYGLLAFVIGGRVSFILQNLPTFLKSPSGIVSINPDIFDAFGGLAAAVIAATIFG
                     QRRGLLLWPTLDALTPFFATLAVGMGLSHLAAGTAFGKATDVPWSIELWSANRHPTQI
                     YETLASLLIFGLIWRQKHDQRPGIHFLTFAALISASQLFLQAFRGDSTLIFNGVRQEQ
                     VVAWLALAVCFMLLEARFKSSPKAETDSAAIHAE"
     gene            complement(18174..18716)
                     /locus_tag="PL000_11250"
     CDS             complement(18174..18716)
                     /locus_tag="PL000_11250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC89521.1"
                     /translation="MQKTQRIILYVLLLIAGAAWTSLSTDKAGTSTSGNIPAPQAGFS
                     APDFSLKPPTGESYTLSELRGQAVLVNLWATWCPPCRAEMPTIEAMYQEYKDQGFIVL
                     AVDMTYQDDPFEVVPFTQEHNLTFPILLEETGDVAAAYQLRSLPSSYFINREGIISEV
                     VIGGPMSEALLRTRIEQILK"
     gene            18797..19558
                     /locus_tag="PL000_11255"
     CDS             18797..19558
                     /locus_tag="PL000_11255"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HNC89522.1"
                     /translation="MLFTDSVFVGVDPTSGRKSFTYAALDKDLRLVALADGELEDVTT
                     FLAGQKSATVAVNAPAGVNRGLVRARMKKEMLTPHQVRGADIRLAEYQLREHGIVVTG
                     TAASAALCPAWMQSGFELYRKLGKAGFKKFPEKEAQFQILETHPHACYCVLAGQIPLA
                     KPSLEGRLQRQLILYENGVYVKDPMDFFEEITRYKLIKGIWPMELLYLPEQLDALVAA
                     FTAWLAVNKPERISTIGDEKEGLIVLPEKELKEKY"
     gene            complement(19636..20871)
                     /locus_tag="PL000_11260"
     CDS             complement(19636..20871)
                     /locus_tag="PL000_11260"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC89523.1"
                     /translation="MKQENSLPQEQIWNIYNCHIHTFTQKNTPGQFYKLLLTDSHSGK
                     MSPLKVVFVLLLPFLLIAFSPLILFMVILDYIRGGAERNSADQNGRMQKVKKKAINRL
                     AALLAAINPASDDLLERYARFLNTSASDSQETIFKQIKHQYPENAKFVILPMDMEFMN
                     LGAMKQSIEDQHADLLKLAAATEGSIIPFYAVDPRHEDIVERVKANLAKDKFRGIKIY
                     PNLGYKPNHERLMDVYKICGDTYPVISHCSPSGIWQYGLSESDRINFGHPENYIPVLK
                     ENKKLRLCLAHFGGAEEWVKHLKGQTPSTGDGRAWVRVISDMLTSGNYPNLYTDISYT
                     IFMPKVNGLYIDLVDYLKVLLSNKQIREHVLFGSDYYMVEQERISEKEASILLRSRLG
                     EDLYRQIAYTNPRKFLGIK"
     gene            complement(20921..21736)
                     /locus_tag="PL000_11265"
     CDS             complement(20921..21736)
                     /locus_tag="PL000_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HNC89524.1"
                     /translation="MVDPNFFNEVPIFELLDDEERKVLADQVSVREFKEGQTVFKAGD
                     PGGLAYLVQKGLIHVTIKDANFEDVTVDVADSGGLVGMSSLLAGENHQTTAVATEDTT
                     AIEIDRNDIMTLLHVKPMAGLDMLTIVEKQLRVAHELMRTRVSKNPNEEIEDHETFGE
                     RMADAVARFGGSWGFVITFAVVLIVYTGINSVLKQPWDPYPFILLNLFLSMLAAIQAP
                     VIMMSQNRQDAKDRLRSELDYRVNLKAELEIEELLHRVGKIEEILVEHKNEDE"
     gene            21820..23784
                     /gene="tkt"
                     /locus_tag="PL000_11270"
     CDS             21820..23784
                     /gene="tkt"
                     /locus_tag="PL000_11270"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNC89525.1"
                     /translation="MTADLQARAINTLRFLSADGVQKANSGHPGLPMGTAAMAYTIWT
                     RHLRHNPRNPKWAGRDRFILSGGHGSMLLYSLLHLTGYDLPLSELQSFRQWGSLTPGH
                     PEYGLTPGVEMTTGPLGQGFATGVGMAIAASHLAANFNQRGHDVIDPFIYAIVTDGDL
                     MEGVASEAASLAGHLSLGRLIYLYDDNHISIDGSTDLAFTEDRAVRFRSYGWQVLRVE
                     DGNDVEAIDKAIQEAKAESRPTLIMCRTTIGFGAPNRQGTSKAHGEPLGDDELNAAKD
                     NLGWPKEPRFFIPDDVLAFYRKAVDKGRELEHDWKMRFEAYARLHPALGAELQRRLNG
                     ELPSDWKSALPSFPADAKGMATRAASGKTINAIAAKLPELIGGSADLAPSNNTKIDGS
                     PAFQKDSYQGRNFHFGVREHAMGAALNGMAVFGGVIPYGGTFLVFADYVRPSVRLAAL
                     SHIPSIFIFTHDSVGLGEDGPTHQPIEHLTSLRLIPNLVVIRPADANETVAAWKVAIE
                     RRNGPTVLALTRQNVPTLETSAQVEKGAYVLKDFGNPEMILMASGSEVGLVLEAAQKL
                     ADEGKGVRVVSFPSWELFEKQDEAYRESVLPNAIQKRLAVEAGSGLGWERYAKSVISI
                     DRYGASAPAKVIFEKLGFTVENVVAKAKEL"
     gene            23817..24269
                     /gene="rpiB"
                     /locus_tag="PL000_11275"
     CDS             23817..24269
                     /gene="rpiB"
                     /locus_tag="PL000_11275"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNC89526.1"
                     /translation="MKVAVGCDHGGFPLKEVVIEAVKAAGHEVIDVGTYSVDAVDFPD
                     FTKKVGEKIQSGEAERGVVICGSGIGAAIAANKMKGIYASICHDTYSAAQGVQHDAMN
                     VLCLGGRVIGPELVKVIVPAFLGAKYLGDEKGGERFAKRVNKIKELEG"
     gene            24304..24714
                     /locus_tag="PL000_11280"
     CDS             24304..24714
                     /locus_tag="PL000_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNC89527.1"
                     /translation="MPTIRRFEEVEAWQTARELTRLVYSITEEGKFAKDFGLKDQIRR
                     ACVSIMSNIAEGFESKTQPAFIRYLGIAKASAGEVRAQLYVANDVKYLSDEQFAQLFD
                     IAEKSSRQLSRFISYLEAQPNTYRVKDESPEYET"
     gene            24782..>27432
                     /locus_tag="PL000_11285"
     CDS             24782..>27432
                     /locus_tag="PL000_11285"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HNC89528.1"
                     /translation="MNPIPKLTSLGQSLWYDNIQRKLLENGELKAMIERGDIRGVTSN
                     PTIFQNAIAKTTDYDAALIPLAWAGWDAERIFWQVAVEDIQEACDLFRPLYDETRGGD
                     GYVSLEVSPFLANDTEGTQKQAKELWERVNRPNLMVKIPATKAGIPAIRSTIAAGINV
                     NVTLIFSLDRYAAVMDAYLAGLEDRIAANESIEMVASVASFFVSRVDTKIDPRLPEGS
                     PLRGRAAIANAKLAYDAFESVFTSPRFATLKARFRARVQRPLWASTGTKNPAYSDTLY
                     VDTLIGPDTVNTVPPATLDAFRDHGKASLTITRGLEEAQLFFAELKAAGISMGSVTQE
                     LEEEGVKAFDDAFKTLLQAIEDRRKSAVSSLGPLADSVAKRLTTLEEHSVAARIWAHD
                     PTLWASDPEGQAEIKIRLGWLHSIEDARTRLDGYLSFAKQIHEEGIDRVLVIGMGGSS
                     LTAEVLSSLMAAANVDAKLSLAILDSTDSEQVALAAKNYPPEKSLYILASKSGGTAEL
                     LAAFDYFWELSKGNGSRFMVTTDAGTSLEKLAKDRGFRKVFNADPNVGGRFSALTDFG
                     LVPAALLGMDIEKLLASAEKIKNASHKNVSAGFALGALLAESALAGRDKLTVLSDAPV
                     SALAGWIEQVIAESSGKHGKGILPVPMEPLGSPDVYGDDRLFVYLRNNGELSDGIAAL
                     KNAGFPVVELPIANLYDAGAEFFRWEIAISVACHILGINTFDQPDVQDSKLRTIAKIK
                     DYQATGKLAEADLVDVADAKSALEKFMTGAQAGEFFTINAYLPRNKETENALQALRVA
                     IRAKTKCAVSAGFGPRFQHSTGQFHKGGPNKGRFIQVVYDAQNDMDIPNQGMTFGTLI
                     RAQALGDYEALVAAGRKVLRVKLNSVE"
BASE COUNT         6302 a         6638 c         7135 g         7357 t
ORIGIN      
        1 agctgtttct tctccattac attgactcca tgagttggga ttttggaatt acagggttag
       61 ggagcaattt aaaaaccctt tgaaccacta agactcaaag acacggagtt gtaattggtt
      121 ttctttgtgt ctccgtgtct ttgtggttaa gattttagct tttcgcagga ggtttgttaa
      181 tcacagacct cccccgttga ctcttgcgta gccttcagca tcatctctac aaccttgaag
      241 tcaatcttct caggcttgga gtagcggatg cagcccttgc cgaggctaat ccctttaact
      301 ttaagcagat ccctatgcgc gtccatcaca tctgtcttga gaatatataa ggcaatgaaa
      361 tttttctggc tggcaaaacc aacttcggct tcaccgttgc gtgtatagac tggcatgccg
      421 tacatcatgc tttcctgaaa ccctttaagc gaagcacggc atagtctgcg caattgggtg
      481 agcgcagctt ttctttcaga aggcgattct ttgagatact ctgtcacatt ttttgcagta
      541 ctttgcatga tgacctcacg gtgtatttat aaaatgggtg atgggtggga tttggtatgc
      601 ttgtaagatt tgctccggcc attttgcggg cgtgaatttg tgcctcttcg atggtcaagt
      661 tggcaaaatg gatcattgtt tcaggtgcat tttttcaaaa cttcatccaa acattttcca
      721 aggctcggat gtaaatttta tctggttcat cgctgtcgac ccatgttttg cctaaatcta
      781 tcaggatttc cacttcaatc aagcttttga caacttcatc tgttgcatca cgataatctg
      841 aaatttcatc gcgagttcct tctggaaaac accattcacc atatgtgtcg gataaaaatg
      901 ctacagtatc acctgtgtgt tgaagcatgt accatgtaac catcgcagca ctaacaggat
      961 tcccagctaa ctctcttgct ttgtttgctc caatatggtt aaaactgatt aattcttttt
     1021 ttgaatagtt aacaagaatg tatccgacac ccatgcaagt tatcctgtaa tctgatttgt
     1081 gtttctctct tattttgcct aacccatgtc gcgattaatg gcttgcactc tctgacagaa
     1141 aaatgctaca gaagccaaca tgacagatgc acgctttttt ggcggcttga tattaattgt
     1201 aaattttatt cgtcgttttt tgtgtttaca ttaattcgtt tgatttcctc tcttgaatta
     1261 agttgcccaa cttatgggtt gtatcgcatc atgctgtaaa ataccccccg tgcggggtgt
     1321 tatacggata tgatccgcat aacagaaatt gcatacaaca ttatacagcc atcatcaggc
     1381 aggaacagag gaaattccct tcttatgcgt ttaaaagcag atctaactct tttggtcgtt
     1441 tcgattatct ggggctcggc ttttgtggcc cagcgtgtgg cgggtcagca ggggagcgtg
     1501 tacctgttca atggggcgcg ttatgtgctg gcggcgctgg tggtgctgcc ctttgtaaag
     1561 cgcgtgaatc tcacatccat gcctcatgat caatataaat ggatgttcat tgcaggtttc
     1621 tttttgttcg cgggtagtgc gcttcagcag gcaggcatgg tttacaccac ggcgggtaat
     1681 gcgggcttca tcactgcctt gtatgtggtg ctcgtgccgt ttgccctgtt tttcttctgg
     1741 cgcgaaaaac cgcactggat gtcggttgtg gcggtggttt tggccggggt gggggcattt
     1801 ctcctttcaa cgggtggcag gttcgaggtc cagaaaggag acgcgctgga agttgtcggc
     1861 gcattgttct ggaccttcca cgtcatcatc atcggaaaat ttgcggcgaa gttcgaagcg
     1921 atgtcatttt ctgtgggtca gcttttggtg tgtggcgtgt tgaatttggg cgtcggtctg
     1981 gtttcagaat cgatgatgac ctttaactgg cagctggcct ttgccattgg gtatacagcc
     2041 ttcttttcgc ttggcttgtg ttacaccttg caggtttggg cccagcagca taccccgccc
     2101 gccgatgccg ccttgatctt gagccttgaa tcggtgtttg cggtgctttc tggctggctg
     2161 ctattggatg aaaggcttgc tgtcattcag atcttcggcg cagtgttgat cttcattgcc
     2221 gtggtgctct ctcaattcaa ggaatggaca tcaggtaaaa ttgaccacga tcaccttgtt
     2281 gagggaagat aacgccatga ctgaagaaag aaacctgatc gaagaactcg acctctcgtt
     2341 ccatccgctc actcaaaaat tgtggcacga ctttgaaatg ttgttcggca ggcgcggcgc
     2401 gtgtggcggc tgttggtgca tgcactggaa attgcgcgga aaggaattca ccgaaagtgc
     2461 tggcgatacg gcccgccaga tgcagaaatc cattgtcgat tcaaagaccg tccccggtct
     2521 gttggcgtat tccgaaggct accccatcgg ttggatcgca gtggaaccgc gcagcgccta
     2581 tccaaaactg gcccattcac gcactctcaa acctgtggac gatcaggaag tctggtcgat
     2641 cacttgtttc tttatcgaga aaaagcatcg tcacaaggga attactgttg aattgctaaa
     2701 agccgccgtg gattatgtga aaggacgggg cggtaaaatt gtggaaggct accccgtgga
     2761 tgcaaagcag gaagcccccg cacctttcgt tttcacgggc acggcttccg cctttcacca
     2821 tgctggcttt gtagaggtcg cgcgcaattc gcccacccgt ccgatctttc gatttgtgat
     2881 cgaataatct tttctttgtt cttttcgtat cctgcataaa taacctcccc tcatcatcga
     2941 gcaaatcatt tgagcgttac ctttcaacct ccttcaaaat catttacagc gcgtgattat
     3001 caattcatcg cgctggtttc aattgtggtc gttgcgattt ccatcggttt ggtgatgctc
     3061 aacctcacgc taaaagatgg cggtgatttt tacgtccact ggtttgcagt gcgcgttttt
     3121 caaaacgaca ggctcgaccc ctacagcgga gaagtccccg cccgtgtgca gcaacaggtt
     3181 tatggaggtc ccgccgcaca aggggatgaa ccctacattc tggatacgcc ctttcacatt
     3241 ctcctgctct actttccgct cacgcttctt tccgacccgc tgctggcccg ggcgatattt
     3301 actttgatct tgcaactggc cttgttcgcg cttgccttca tcagtctgcg tctcaccgaa
     3361 tgggaagtgc cgatcatttt ctacgtcctg tttcccctgc tggctatttt caatttttat
     3421 acctttcaag cgatctacga agccagtccc gttctattgc ttgggctcac ctacgccgaa
     3481 attttgatag ccctccgtgc cgatctggat gaagtggcag gggcccttgc tgcggtctca
     3541 ctgtattatt gggaagttgg tgcgccgttt cttgtccttg tggccttgcg ggtgtactac
     3601 gaaaagcgca ctcgcgtttt tgcagggttt gccatggcta gtctcattct gttggtgctt
     3661 tcattcatgg cctatcccaa ctggttcatt ccgtttttac gggccacggt caacaatatc
     3721 aaagcggatt ttggtttcaa cgtccattcc attttcatcc gcatctggcc tgcgtttgga
     3781 ggtacatttg catggatgtt cattggcttg ttggtggtgg ccctcggcta cgagtggagc
     3841 gctgcccgca gcggcgattt ccgccgtttt tattgggcgg cctgtctttc cattgcggcg
     3901 gctcctttgc tgggctttcg caccgaaatg gaaaacctcg ccgtgctgat cattccgctg
     3961 gccctgatct tcgccatcgt ccatgaccgc tggtacaaga tcggtaactt cctcaccata
     4021 ttgttgatgc tgcttgtcgg cactctgccc tgggcggttt ccctgttcct tgtcccccgt
     4081 tttggaatca aagccgaaga aggtttgttc ctgtttttgc ctcttttcac tttcattggc
     4141 ttatactgga ttcgctggtg ggcgatccgc ccgccgcgca ttttgagcga tattgccagc
     4201 cgcctctaac cccattccat gcctatgaac tcgcgcacct acttcttcct ctttctcttt
     4261 gggcttgctg ttcccgtctt cattgcccag ttccagccca tcccgggcta tctggattcg
     4321 gattattact atgcaggcgg aattcaactc gccaccggca aaggcttcac agaaccctac
     4381 ctgtggaatt ttcttgatgg caccacgtca ctcccgcatc cctcgcatag ctattggatg
     4441 cccctggctt ccattgtctc tgctttgggc atgtggctga ccggtcaaac cacctttgct
     4501 tcggcgcgtt tgttcttttt cctgatcgcc gcccttgtcc ctccgttgac cgctgcgctg
     4561 gcttacgctt tttctcaaaa aaacgaactg gccattgcat cagggatgct ggccatcttc
     4621 tctgtttaca acgccccctt tgtcggcgtg acggataact ttggtctttt catgttgttt
     4681 ggcggcttgt atttcatcat cgccacgcag cttatgcaaa accccgaccg aatgcgcaat
     4741 tggctgtttc ttggcctgct ctctgggttg atggctttct cgcgcagtga tggtctgctc
     4801 tggctgccgc ttacatttct attcgcctta tggcgtgcca gaaaaatttc atccttcatc
     4861 cctcatccct catccctctt gttgattctg cttggctttc tcgccatcat gtcgccgtgg
     4921 tatctccgta acctgaattt gtacggtacg cccatggccc ccggtggcag ccgcgcactc
     4981 tggctcgaaa actacgacca gacctttatt tatccgccga ccctgttaac aaaagaatcg
     5041 ttcctcgccc aaggctggga aaaaattctc gacgaccgcc tctgggctct gaacatgaat
     5101 ctgcaaagcg gtttcgctgc gcatggcgga atcatattat ttccattcat cgtggctgga
     5161 atcatttatt atcgtaaaga tgatcgcgtc aaactggcgg cactcgcctg gttgattctt
     5221 ttcctggtga tgacctttct ctttcctttt gcaggtgcgc gcggggcatt tttccatgcc
     5281 ggggcggcgc ttcagcccat atggtggacc cttgctccgt tggggcttga gtcgatcctt
     5341 gtgtccctga gaaagcgcgg ctggggcaac gaccagaatc ggttcgtgtt tcgaagcgca
     5401 ctggtcatga tcgccatgat cctcactgtg tatgtcgtct atctgcgggt gttcacactc
     5461 ggctggggcg aaatcgacgc cgattacaag gctgtagatc aattccttgt tgagcatggc
     5521 gttggcgaag aggatattgt catggcacgc aatgcgcctg ggtattacct gctgacaggc
     5581 cgctcggcaa tttccattcc atacggcggc gagaatgcgc ttttggaagc tgccaaacaa
     5641 ttcgacgcct actatctggt gttggaacct gaggccgctc tcgacccgat caaggattta
     5701 tttcaaaatc ccaacagccg caatcggttt gtgtatctag gtgatgttga tggtgcgcgt
     5761 atttacaaac tcgaaactaa ataagatttc aaaccgtgat ctgttgatgc tggctgccgc
     5821 agtggtggtg gttacttcca tttatcttct cgcaagttat tttaattacg gcatcggctt
     5881 ccccttggac gattcatgga ttcaccaaac ctatgcccgc aaccttgccc aacgcggcga
     5941 atgggcattt cgccccgggg ttccctccgc aggttccact gcacctctgt ggtcggcttt
     6001 gcttgcgctt ggttttcttt tcagcctttc gccatatatc tggacatatc ttcttggcgc
     6061 attcaccctc tttgcattaa ccgttgtttg tgagtgggcc gtacgtaccc taatcgattc
     6121 ataccgtcca cacctgccat gggtcggaat tttctttgcc tttgaatggc attttgcctg
     6181 ggcggcaatg tctggcatgg aaaccctgtt gcacggattg atcgtcacaa cagttttggt
     6241 tttgctgatg acgaacacgc gtcgctactt aaccctgggc ctgctcaccg gtctatcggc
     6301 ctgggtccgc cccgatggtt taaccctgct cggccccgtc ctgatcatgg ttctgatcca
     6361 tgaacaggat acccgtccgc gtttgattgc tttgctgcgc tgcctgatcg gttttggttc
     6421 attattcgtt ccatatttgc ttttcaatct ggccattggc ggcactccca tgccgaacac
     6481 cttctacgcc aaacaggctg agtacgcctc ctggcagacc atttccattt tttcgcggct
     6541 tgggcaaatg tttctgcaat tgctggttgg accaagcctt gtgttgatcc cgggcgttat
     6601 gggttggctg gtcaaaagca ttaaacagaa aatgtggggg agccttgccg cgctggtttg
     6661 gtgtgcagga tattttgggt tgtacatttc ccgactccct gtctatcaac atgggcgtta
     6721 cattatgccc gccatgccca ttttctttct gttcggtctg ctggcctttg ccgatttcga
     6781 aaaaagcagg ctcttcgaac gctatcattg gatcggtcaa accatctggc gtgcaagcct
     6841 tgtcatgctg tccgttgctt ttatttttct tggcgcccaa tcctactcgc aggatgtggc
     6901 cttgatagaa agtgaaatgg ttgtaaccgc aaaatgggtc gccgccaatc ttccacccga
     6961 tgccctcatc gccgcccacg atattggcgc gcttggtttc ttcgacaccc acgcgctgat
     7021 cgatctggct gggttgattt cgcccgaggt catcccgttc attcgagacg agcccaaact
     7081 ggcaaattac ctcaaccagc agggtgccga ctatttaatc gcctttcccg gcttttaccc
     7141 agccatgaca ggcagtctgg agaaggtttt tgtaacaaac agcgtagtca cccttaagta
     7201 cgatgaggag aatatggctg tctatctgtg gaaaacccct taatgggtta aaatatgaat
     7261 aagcaacaaa atcaaaaaaa gagagagaat gggccaggga tgaaccagat tacacttttg
     7321 atcgttgatg atcatccatt gtttcggcag ggagtggtcg atgccttgtc attggaaccc
     7381 gatatgcgca ttattgccca gtctgccgct ggtgatgaag cgctggagtt gatccgcacc
     7441 aaaaagccaa ccatcgctgt gatggatgtg aacctgcccg gcatgaacgg tcagcaaatc
     7501 acccatcagg catctcagga tagactgccc acccgtatta tcctcatgac aggatatgac
     7561 gacgtggaac aagccatcca tgccgcactg gcgggtgctc atgggtactg ctccaaagat
     7621 attgagcctc agagcctttc gcgcatcatc cgcgaggtgg ctgaaggaaa atatgttttt
     7681 gaaaataacg ttttcacccg tcgtgaattg gaactttggg tggaggacag gatcgaagga
     7741 gcccgtcgtt catacagtga acctggcact cccttccatc cattgtctga ccgagagatg
     7801 gaagtccttg cttgtgttgt tcgtggcatg agcaacaaag aaattgccac tctgttgggg
     7861 atcagccacc aaacggttaa gaatcatgtc actgccattt tgcgcaagtt tggtgttgaa
     7921 gatcgtaccc aggcggttgt gtatgccttg aagcgtgggt gggtaacctt gaaggatacc
     7981 aatattcacc cccaggagta ataaatgacc agtaccgagc ctatggattt tcagagcgaa
     8041 ttggatgaaa cacagcgtgc tcttcgtgaa gtgaccttga tgatcgaaca gagtcagggc
     8101 gaacttggca agatcaccca gcgaaacacg gcgatcactt cgcatcttca gcagttgcag
     8161 aaacagggga caactgttga tgatctgaaa atgtcatacg attcggcttt ggatgttcag
     8221 cagcggcttt ttgtaatgcg gggacaattg gaaaaactgc aaaacgataa aagccatctt
     8281 gagaaatata aaaccgtttt ggagaatatc gtttccgggg cagcttcagg cggagatgcc
     8341 gccggggcga ctcctgccgc aggtggcaaa agccagatgg caggtataga aatgatcgtc
     8401 aatgcgcagg aggctgaacg tcagcgcctt tcacgtcaaa tgcatgatgg tcccgcccaa
     8461 gccttgtcaa attttatttt acaaactgaa attgccatgc gtttgctgga tgttgaccct
     8521 gcacaggcca aggatgaatt gggtaatttg aaaacctcag ccatgggcac cttccaaagg
     8581 gtgcgcagct ttatttttga attgcgccca atgatgcttg atgaccttgg tttggtgccg
     8641 accctgcgaa aatatgccga tgcatttaaa gagcaggcca gtcttgatgt gagtgtgact
     8701 gtcactggca cagaacgtcg tttggaacct tatttggagg tcatgatttt ccgcgcggtg
     8761 caggaattgc ttggcaatgc ttcccgtcac agccaggcta ctttgatcaa ggttcaaatt
     8821 gaccttggga acgacacaat ccgtgtcagt gtggaagaca atggcaaggg tttttcacct
     8881 gaaatcctga aggaatccag caaccttggt ctgaaactaa tccgggagcg ggcggaaatg
     8941 ttaggtggta gcttcgaggt tgatagtgcg gccggttctg gtacaaggat tgcattttcc
     9001 gttccggcaa aggtgtaact tgaaaagaac gcatcccaat cgcctacaat attgttaggc
     9061 aattgggatg cgtggtactt gttttgtatg gtgggacaaa catataatac ggaatatttt
     9121 ctttagggga tgttcatttt gactgatctg ccatggcttg tatcgaagag aacgttcttc
     9181 ctgccgtaaa actggctcga aaaggcacga aattatgttt ttggcatttt tcttgtataa
     9241 tgaatatgtt ataaaatgct ctatcaaaga atgtgaaaag aggagagtcc tatgcgtcgc
     9301 ggaagaaccc tgatttttgt agtactaata ctaattatcg gtttggcggt ggcagtggcg
     9361 gcatactttc agtttctgag gccagcggct acacccacac agccagtaac aaccgttgat
     9421 gtatatatgg ctggtcagcc aattgcacag ggtagtgaaa tcacgcctga gttgcttact
     9481 acgatcaaaa ttgcacctga gaatctggtc tcggtaatgt ataccgtgga aatgcagtca
     9541 cagcttatcg gcaaagttgc caagtatcca ctggatcaag gtgtcattat tacccaggcc
     9601 atggtgagtg acgcaacctc ggctgttccg atctctggcc ctcaatgggc ggctttgatc
     9661 cccaccggaa tgacagcgat ctccatccct gcagatagat tgtctttagc cagttatggt
     9721 gttaacaatg gagcccatgt taatgtcaat gcctgtttct tgtttgtaga tattgaccca
     9781 tctttccaat ctgctttacc gaatcagacc ggtcaattaa ctggtaccgg ttttacgcct
     9841 gaaggattgc ctgtactttc acttggtgtt tctgccagtg gctccagtca gggtcgcctt
     9901 gaactcgacc catcgattca acagccctat tacttgatgc cgtcggaacg tcaacgtccg
     9961 cgtatggtgt gtcagatgtt gttgcaggat gttgtagtga tgaaagttgg tgatttcccg
    10021 tccgacccgg ctgcgagtct ttctgcgccc gcacctgtcg caacagaccc ggagactcag
    10081 caagctgcgc cagttccacc cgatgttgtc acattgatcg tactccctca ggattctgtg
    10141 actctttcct atttgatcca cacagatgcg gaattgacct tgactttgag aaatccgacc
    10201 gatcaattgc gccaggcaac cgaggcttca actttgcaat tccttctgag ccaatacaat
    10261 atcccagttc ctgcgaaatt gccctactct cttgagccat atactgtaag tttttgtaat
    10321 ctcagtcttg gtacttcggc aatttgttct caaacagcag ctccgcccca gtagttacag
    10381 ttttttttta ctggagatca tatttgtatc ccgttgttgt tttaggcccg gcggcacaaa
    10441 aaggtagaaa aatatagaaa ttagtgagat tagacttgaa ctttcgaaat gaatggaagc
    10501 ataaacatgg ccgcagataa aattcgcatc ctcattgtag atgatattgc agagacgcga
    10561 gagaatattc gcaaactgtt gcagtttgag tcagacgttg atgttgttgg tgtggcgcga
    10621 tctggaaaag aaggtattca acttgctcag gagcttgatc cagatgttgt attaatggat
    10681 atcaacatgc cggatattga cggtattact gcaactgaac aggtgcgtaa gaaatcaccc
    10741 catgtgcaag tggtgattct gtctgtgcag aatgaccaaa gttatatgcg acgtgccatg
    10801 ttggctggcg cacgggattt tctaaacaag ccgcctttgg gcgatgaatt aatttccgct
    10861 attcgccgtg caggggaaat ggcacatacg gagcgggcaa agagcgccca gcagagtttt
    10921 gtaggagcgg ctgtggctgg ctctggcggg gctgcaatga attttgcccc ggtgcataag
    10981 ggtaagatca taaccttata tagtcctaaa ggtggaacag gatgcaccac tctggcggta
    11041 aacctggcga ttgcgttaaa caatgaagat actcgcacat ctcttgtaga cgcaaatctg
    11101 cagtttggcg atgtagccat ttttgtgaat gaacctggca agaatgcgat cattgaactc
    11161 gccccacggg ttgatgaatt ggaacccgag gtcgtggatg atatcctgat caatcatgga
    11221 gcttccgggg tgcggatttt gaccgcccca caacgccccg aatatgccga aaagatatcg
    11281 gctgagcaat ttaccaaggt tatccagtat ttacagcgga tgtatgcata cgtggttgta
    11341 gatacatcgc acattttgtc ggatgtcgtc ctgtctattt ttgatgtaag tgatgtcatc
    11401 gtgttggtga ctacgcagga gattccagcg atcaagaacg ccagattatt ccttgacctt
    11461 ctgcagacca tgggtattaa caagaatcgt gtcattttta caatgaatcg ttacgataaa
    11521 cgcatttcaa tcactcctga acgagtgagt gagaatctta agcacgaagt atctgttaca
    11581 atacctttgg atgaaaagat cgcgattact gctgttaatc gtggagtgcc atttatgctc
    11641 gataacaaat cccagcccat tggaaggggt atattctccc ttgcagaggc ggtccgaagt
    11701 cgtttgacaa ctttagaatc tgaaaatgat atgtctgata gacggtaagg agagttacta
    11761 tgtcgttgct tcgacgtatt gaacaggggc agcagggggg tggacagcaa cagggaaatg
    11821 gtaatacgag tgggactccc tcgccgacac ctccggcaaa gcagccaggt tcagggcagg
    11881 gtggagaagg ttctggcggt ggaggagata cctcccgcct gtcttcatta caggcaaggc
    11941 gtgtcagcgc tccgatcact tcccctcaag ctggatctta ttttgattta aagacccgag
    12001 ttcaaaacaa attactcgct gagatcgacc cgtccatgga tgtatccagg acggacgatg
    12061 ttaagcgtac cattcaggga ctgttcgagc agattcttac tgaagagaat atcgttcttt
    12121 cgagacctga acgcgcgcgt ttgtttgagc agattgcagc agaaatcctt ggtctgggtc
    12181 cattacaggc actgcttgag gacgacacca tcacagaaat catggtgaac ggccccaaaa
    12241 atatctatat tgaacgtaag ggtaaactcc atcgcgttcc ggtgaccttt gaaagcaacg
    12301 agcacgtgat gcgtatcatt gatcgtatcg tggccccgtt gggacgccgt atcgatgaat
    12361 cgagcccata tgtagatgct cgtttgcagg acggttctcg tgtaaatgca gtcatccctc
    12421 cgatttccct gattggtccg gtgttgacca tccgtaaatt ctcaaagaat ccgatctcaa
    12481 ttgagcagat ggttcagttt ggttccattt cacatgaggc agtgcaattt ttgaaggctt
    12541 gtgtggaagc acgattgaac attgttattt ctggcggtac aggttctgga aagacaactt
    12601 tgttaaacgt tctctccagc tttattccag gagatgagcg tattctgacc attgaaaacg
    12661 ctgctgaatt gcagcttcgc caggaacatg ttgtaacctt ggaatcccgc ccgccaaaca
    12721 ttgaaggccg cggtgaaatt accattcgcg acctggtgat caatgccctg cgtatgcgcc
    12781 ctgaaagaat tatcgttggt gagtgccgtg gtggagagac cctggacatg ttgcaggcca
    12841 tgaatacggg tcacgatggt tccatgacta ctgcccacgc aaactcgccg cgtgatgcga
    12901 tcgctcgtat cgaaacgatg tgcctcatgg ctggtatgga tctgcccgtt cgtgcgattc
    12961 gtgaacaaat cgcaggtgcg gtggatgtca tctgtcaaca ggctcgtatg cgtgacggta
    13021 gccgtaaagt aatcgccatt actgaagtaa gtggtatgga aggcgatgtt attaccatga
    13081 cagatatctt cacttttgaa caaactggca tggaaaacgg caggattatc ggccgaaccc
    13141 gtcccaccgg gttacgtccc aaatttatgg ataagattga ggctgctggc attcatttgc
    13201 ccccatccat ttttggtatc ggtgaaagaa gaagatatta aaaatctgac gggttattaa
    13261 aaacctatgc ttaacataat tatcattggc ggtgttgtct tgatcattct gttgatcatt
    13321 ggcgttgttg tttcttcaaa cagcgaacgc gcagtggtcg agcagcgtct tgctcagtac
    13381 ctggatgatg acaaacagga tattgacagt gcggctcaaa actctgtttt gacagagtgg
    13441 gtctcgaaaa gggtggaaaa aacatccttt ggtgaccgta tcgccagaga ccttgcccgt
    13501 gcggatctga agtttaaggc gggtgagtac tttattttga tcattgttag catcttgctg
    13561 ggcgggcttg tcatctggtt tttggggaat caacattggg cgtctgctgt catcggtgct
    13621 gtggctggtg cctttgcccc gggaatctat gtaaggcgtc aacagaagca gcggttgcag
    13681 aagtttaacg accaactttc agacatgctc aacttgatgg taaatggtct gcgggctggg
    13741 tattccacaa tgcaagccat ggaagcagtg agtaaggaac ttccatcgcc aattaacgac
    13801 gaattccgtc gcgtagtgca ggaaatgcaa atcggtattc ccatggaaac tgcattggat
    13861 aaccttttgc gccgcattcc aagcgatgac ctggactttg ttgtaacggc cattaatgtg
    13921 cagcgtgaag taggtggtaa tctttctgaa attttggata ccatctcatt caccatccgt
    13981 gaacgtgtcc gcatcaaggg cgaggtcagg gtgcttactg cccaagtgcg tacttcagga
    14041 tctgttctct ctcttattcc gtttttcctt gtattaatcc tttggttcct taaccctgag
    14101 tatattctgg ctgttacggc aggtggacct attattacgg cagccattat ttgtgtggtt
    14161 cttggtttga ttttcactgg ttactttatc atgatgaaga tcgctgatat cgaggtgtaa
    14221 ctatgggtac tattgttggg atcattctgg ggatcgttgt ggtaggtgga gtgattgcac
    14281 tcatcgtagt tgggatgcgt gcccgtcagg gaggtgggga cgaatctgac cccatattgt
    14341 cccgtttggc agaggcaacc cagcgcggtg agacggtatc ccttgaagat atcgagttac
    14401 aacaaccctt tattcaaagg gtgatcgtgc caatgatcga aaaggtgggt gaagtttcat
    14461 cccgcttcac accgcaaaag cttttgcagg agaccactct taaactggag ctggctggta
    14521 accctggtcg tattgatgct gcgacgttcc tggcgacccg cttttttggt gctggtatct
    14581 tcggtggtgg tctgttattg atcgctttgg cctctcaaac ctggccggtt ggtagaatca
    14641 ttttggtggt tgggttcttt actgttattg gtttcttctt cccgcagttg tggttgcaga
    14701 gccgcatcaa tagccgtcag gcggaggtgc gcaaggcatt gccggacgcg cttgatctgt
    14761 taaccatttg cgtggaagca ggtctgggtt ttgatgcagc catgtccaaa gtggctgaaa
    14821 aatgggagaa cgaactctcg ctgatgtttg gacgttgtat tcgagaggtt cagcttggaa
    14881 agacccagcg cgaggcattg cgtgatatgg cagaccgcct tggcctgcct gagttgacca
    14941 gttttgtcgc cgccgtgatt caaagccaga tcctcggtgt gagtcttgcg aaggtgctgc
    15001 gtattcaatc ggaccagctg cgcgtcaaac gccgtcagtt cgcagaagaa ttggcacaca
    15061 aggctccagt aaaaatgatc attccgatgg ccttgttgac ttttccctcc atcatgatta
    15121 ttttgatggc tcccgccgcc ttccagatcg caggcgcttt tgggccattt ttaggtcagt
    15181 aaaaaaactt ccagcctttc ttttgcttgt tttgcaaacc tgcacaagca aaaaatggag
    15241 gcgcagtttg tctaatagat cattggcata taatctttac aggtcattat gggctggttt
    15301 ggacctcctg tatccaccgc aatgtggcgg atgtgggaaa actggctccc gttggtgtgc
    15361 ggaatgtcaa cagcgggtgc aggtcctgaa tggaaatctg tgtgaagtat gcggccttcc
    15421 ccgggaaagc gcaggcgtct gtgacacctg ccttgcggac aggccgcatt ttcattccct
    15481 gcgtgcatgg accatattcg aagacccgat gcgcaatgcg ctgcataaac tgaaataccg
    15541 acgcgacctg gccctggggg atgcacttgc cgcacatatg tctgattttg tgcgcacctt
    15601 gaattggcag gccgatatga tcgttcccat tccattggga aaacagcgat atcgcgaacg
    15661 gggatataat caggttgcaa tgatcgcaaa accgctggca ttgtcgcttc gaatggaatt
    15721 tgcgccacag accctgagcc gtcgtaaaga gacacgctca caggtggggt tgaccaagga
    15781 agaacggcgt gtaaatgttc acgaagcgtt tcaagcaggg acgggggtaa aaggaaaaaa
    15841 tgtcattgtg atggatgatg tctctacaac aggatcaacc ctctcgtcca gtgccgaagc
    15901 gttattccgc tctggagcca gaaatgtatt tgcgttaaca gtcgctcgtg ccctgcctca
    15961 tcacggtctg aggcatgcat gagcgttttg tgttcgtaac ttttcatccc tataaggagg
    16021 acgtatgtcc aacaaagtgg aagttcagac acgcaacatc cgtttgaccg aaaaaataga
    16081 agagtatgtc aacaagaaag cgggtaatct tgaccgttac ctgcccacca ttgactcggc
    16141 ccgtgtagag cttgctcatc accaggctgc gcgcaatgcc accgaccgta atgtggctca
    16201 aattaccgta tatggaaagg gattcaccct tcgcacggaa gaacgtgccg acgaagccct
    16261 ggctgcattt gaccgcgcca ttgataatat gcagacccag atcgaacgct acaaagggaa
    16321 acaccagcat ggccgtggag atggacgctc tgctgcagag gccgccgaac ccatcattga
    16381 cgatgagacg ggtgaactct ctccgcttgt agcccgcaaa aagaaattcg tgttgtatcc
    16441 gatgaacgaa gaggaggctg tcgtccaaat gcgcaacctt ggacacgata acttctttat
    16501 cttttataat gctgaaacca gcaagatcaa cgttttatac cgccgtcgaa acgggagtta
    16561 tggattgatc gagccagagc tcggctaatc ggtttaccaa taactggctt ggcggctatg
    16621 ctgccaggcc agttttaatt tatcaaccat cggcttgaaa aaacgatggt cggagtgaaa
    16681 atggaatttg atgatgtgaa cgagagcgat gttgaaggat cggccattga caatgccgca
    16741 atagaaaccg ccgtgactca gattttgaat gctgtgggcg aagacccgca gcgcgaaggt
    16801 ctgcaattca ccccgcgccg cgtggcgcgc atgtatcacg aactgcttgg cggctacaaa
    16861 atggacccgg ttgccatcat caacggcgca ttgttcaatg tgaaatacga cgaaatggtg
    16921 ttggtgcgcg atattgagtt ttacagcctg tgcgagcatc acatgctgcc tttcatgggc
    16981 cgtgtgcatg ttgcctatat tcccgatggc aaagtgcttg gtctttcaaa gatcccccgc
    17041 attgttgaaa tgtactcacg ccgcctgcaa gtgcaggaac gcatgacacg tcagatcgca
    17101 gattttttgc gcgacctgct caaaccacag ggcgtggccg tcgtcgtgga ggccatgcac
    17161 ctgtgctcca tgatgcgcgg cgtaaaaaaa cacgacgccc gcatgaccac ctctgccatg
    17221 catggtgcat ttcgcgccaa cctcgccaca cgccaggaat ttcttgcaaa catctcacgc
    17281 ggctcggcac ccttacagct ttagaaaaaa cgcggcctca acaacaaaaa atgcggaaat
    17341 cggtttccgc atttttttta ttttcatata gggaatgaaa acccgaatca gacttaacct
    17401 cagcctgctt ctgtcattcg gcatggatcg ccgccgaatc agtttcggcc tttggcgaag
    17461 atttgaacct tgcttcgagc aacataaagc agaccgcaag cgccagccat gcaacgacct
    17521 gctcctgccg caccccgttg aagataagcg tgctatcgcc gcgaaatgcc tgtaaaaaaa
    17581 gctgcgaggc agagatcagc gcggcaaagg tgaggaaatg aatgcctggg cgctggtcat
    17641 gtttttgacg ccagatcagg ccaaagatta agagcgaagc gagcgtctcg tagatttgag
    17701 tcgggtggcg gttggcgctc cataattcga tgctccaagg cacatcggtt gcctttccaa
    17761 aagccgtacc tgctgcgaga tgcgaaaggc ccattccgac ggcgagggtt gcaaagaatg
    17821 gggtcaatgc atcgagggtg ggccagagta aaagtccgcg ccgttgaccg aagatggtcg
    17881 ccgcaatgac tgcggccgcc aatccgccga aagcgtcgaa gatatcgggg ttgatggaga
    17941 caatgccgct ggggcttttt agaaatgtgg gcaggttttg caggatgaac gaaacccgac
    18001 caccgatgac gaaagccagc aagccataga agatgacatt attgaggtcg tctttttgga
    18061 tgccgtggcg ctcggcgcgc ttttcggcga aggataagcc gaaccatgcg gcggcaacca
    18121 gcaggatcat gtggcggggc ggggaaaata gattgcggaa gagttcgatc atgttatttt
    18181 agaatctgtt cgatacgggt gcgcagcagg gcttcagaca tggggccgcc gatgacgacc
    18241 tctgagatga tgccttcacg gttgatgaag taggaagatg gcagggaacg cagttgatac
    18301 gcggctgcga catcacccgt ctcttcaagc aggatgggga aggtgaggtt gtgttcctgc
    18361 gtgaatggaa ccacttcgaa ggggtcgtcc tggtaggtca tatctaccgc gaggacaatg
    18421 aagccctgat ctttgtattc ctgatacatg gcttcgatgg tgggcatttc agcacggcag
    18481 ggcggacacc aggtagccca gagattgacg agtacggctt gtccgcgcaa ttcagagagg
    18541 gtgtaagact cgcctgtggg gggttttaat gagaaatcgg gtgcggaaaa acccgcctgt
    18601 ggagcgggga tgttccctga tgtggatgtg cctgccttat ctgtgctgag cgaagtccat
    18661 gctgcgcctg cgatgagcag gaggacgtaa agaatgatgc gttgagtttt ttgcatgatg
    18721 tctgagtata catgaattgg ggtgtaaaat agaccagtcg gttaatctgt ctacttgatt
    18781 cttttataac ttacccatgc tattcactga ctctgttttc gttggcgttg accctacttc
    18841 gggacgtaaa tctttcacct atgccgcatt ggacaaggat ttacgtctgg ttgcgcttgc
    18901 cgatggtgaa ctggaagatg tgaccacatt ccttgctggg cagaaatcag caactgtggc
    18961 ggttaatgcc cctgctgggg tcaatcgcgg gttggtgcgt gccagaatga aaaaggaaat
    19021 gctgacgccg catcaggtcc gcggtgctga tattcgtctg gcggaatacc agttacggga
    19081 gcatggaatt gttgttacgg gtacggctgc aagcgcggcg ctttgtcctg catggatgca
    19141 atcgggtttt gagttgtatc gtaagttggg gaaggctggg ttcaaaaagt tccctgaaaa
    19201 agaagcgcaa ttccaaattt tggaaacaca tccacatgcc tgttattgtg tgctggcagg
    19261 gcagattccg cttgccaaac cgtccttgga aggccgcctg cagcggcagt tgatattgta
    19321 cgaaaatggg gtgtatgtca aagacccgat ggactttttt gaggagatca cccgctacaa
    19381 actcatcaaa gggatctggc cgatggagtt gttgtacctg ccagaacagc ttgatgccct
    19441 ggttgcggcc tttacagcat ggctggctgt gaacaaaccc gagcggattt caacgatcgg
    19501 cgatgaaaaa gaagggctga tcgttctgcc tgaaaaagaa ttgaaggaaa aatattaggt
    19561 tttgcaaaaa aacttgagct taaacagcag agctacagat acattctgta gctctgtttt
    19621 gtttgcggtc aaaaactatt taattcccaa aaattttctt gggttggtat aggcgatctg
    19681 tctgtacaga tcttcaccga gcctgctcct cagtaaaatg gacgcttcct tttccgagat
    19741 acgttcctgc tccaccatgt agtaatcgct gccgaagagt acatgctccc gaatttgttt
    19801 gttcgaaagc aggaccttga gataatccac gaggtcaatg taaagcccgt tcaccttggg
    19861 catgaagatg gtgtaggaaa tatcggtgta gaggtttggg taatttccgc tggtaagcat
    19921 atcagaaatg acccgtaccc aggcgcgccc gtctccggtg gatggcgtct gcccttttag
    19981 gtgttttacc cactcctctg cgccgccaaa gtgagcaagg cacaaccgca gttttttatt
    20041 ttccttcaaa acagggatgt agttttcagg atgcccaaag ttgattcggt cagattctga
    20101 aagcccgtat tgccagattc cgctgggaga gcaatgcgaa atgaccgggt aggtatcgcc
    20161 gcagattttg tacacatcca tgaggcgttc atgattcggt ttgtatccga ggttggggta
    20221 gatcttgatg ccgcggaatt tgtcttttgc aagattggct ttgacccgtt ccacgatatc
    20281 ttcatggcgc gggtcaacgg cataaaatgg aataatactt ccctctgtag ctgcagcaag
    20341 tttcagcaag tcggcgtgtt ggtcttcaat tgactgtttc attgccccca ggttcatgaa
    20401 ctccatatcc atgggaagga tgacaaactt tgcattttcg gggtattggt gtttgatttg
    20461 cttgaaaatg gtttcctgtg agtcagatgc tgaggtgttg aggaaacgcg cataccgctc
    20521 cagcaggtca tcagaggctg ggttgatggc cgccagcaaa gctgcaaggc ggttgatcgc
    20581 ctttttcttc accttctgca ttctgccgtt ttgatctgcg gaatttcttt ccgctccgcc
    20641 tctgatgtaa tccagaataa ccatgaacag gatcagcggc gagaaagcga tcaacaggaa
    20701 tggcaacaac agaacaaata caaccttgag cgggctcatc tttccgctgt gtgagtcggt
    20761 tagtaacagt ttgtagaatt gcccgggcgt attcttttgc gtgaatgtgt ggatgtggca
    20821 gttatagatg ttccatatct gctcttgcgg aaggctgttt tcctgtttca tggggcctcc
    20881 tttatttgga tctttctgag gtgtggattg tgtttactgt ttattcatct tcgtttttat
    20941 gttcaacgag aatttcttcg atctttccaa cacggtgtaa aagttcttca atttccagtt
    21001 cggctttgag gttaacacga taatcgagct cgctcctcaa acggtctttc gcatcctgac
    21061 ggttttggct catcataatg accggtgcct gaattgccgc cagcatggaa aggaatagat
    21121 taagcagaat aaacggatac ggatcccagg gctgtttaag tacagaattt atgccagtgt
    21181 acacaattaa aacaacggca aatgtgatga caaatcccca tgaaccgcca aatctggcaa
    21241 ctgcgtcagc cattctttcg ccaaaagttt catggtcctc gatctcttca ttcgggtttt
    21301 tggaaacgcg ggtgcgcatc aactcatggg ccacgcgtaa ctgtttttcc acgatggtca
    21361 acatatccag cccggccatg ggtttgacgt gtaaaagggt cataatgtca tttcggtcaa
    21421 tttctatggc ggtggtgtct tcggtcgcga cagcagtggt ttggtgattt tcgccagcca
    21481 gcagtgacga cattccgacc agtccgccgc tatctgccac atctacagtg acatcctcga
    21541 aattggcatc tttgatcgtg acatgtatca gacccttctg taccagataa gccaacccgc
    21601 cggggtcacc agctttaaat acggtctgcc cctctttgaa ctcgcggaca gaaacctgat
    21661 ctgcaagtac ctttctttct tcatcatcga gtaattcgaa aatgggaact tcattgaaga
    21721 aatttggatc aaccatgaca ggcaacctcc tttttgattt agttaaagca tatcatcgtt
    21781 tggtaaaata gtgaaaaatc ttttttacag gacttaatca tgactgctga tttgcaagct
    21841 cgtgcaatta atacccttcg ttttctctct gccgatggcg tgcagaaggc caactctggt
    21901 catcccggcc tgccaatggg gactgcggca atggcgtata ccatttggac ccgccacctg
    21961 cggcacaatc cacgcaaccc aaagtgggcg ggacgtgatc gcttcatcct ttcgggtggg
    22021 catggttcca tgctccttta ttctttgttg catctgacgg ggtatgacct gccgctctcc
    22081 gagttgcaaa gtttccgtca gtggggcagc cttaccccag gccaccccga atacggcctg
    22141 acccccggtg tggaaatgac cacggggccg ctcgggcagg gatttgcgac aggggttggc
    22201 atggcaatcg ctgcttcgca ccttgctgcc aacttcaacc agcgtggaca cgatgtgatt
    22261 gacccattca tctatgccat cgtcaccgat ggcgacctga tggaaggtgt ggcctctgag
    22321 gctgcgtcgc ttgcggggca tttgtcactt gggcggttga tctatctgta cgatgacaat
    22381 cacatttcca ttgacggctc aaccgatctg gctttcactg aagatcgtgc ggtacgtttc
    22441 cgctcgtacg gttggcaggt tctgcgtgtg gaagatggca acgatgtgga agccattgat
    22501 aaagccattc aggaagcgaa agccgaatct cgcccgacct tgatcatgtg tcgtacaacc
    22561 attggctttg gcgcgccaaa caggcaggga acttccaaag cgcatggtga accgcttggc
    22621 gacgatgaat tgaatgctgc gaaagacaac cttggctggc cgaaggaacc acgtttcttc
    22681 attcccgatg atgtgctggc gttttatcgc aaagccgtag acaagggccg tgaactcgaa
    22741 catgactgga agatgcgctt cgaggcatat gcccgtctgc accctgcact gggtgcggaa
    22801 ttgcagcgcc gtttgaacgg cgaattgccc tctgattgga aatcggccct gccgagtttc
    22861 cctgctgatg ccaaaggcat ggccacccgt gcggcctctg gcaagaccat caacgccatt
    22921 gcggcgaaac tgcccgaact cattggcggc tctgcagatt tagccccctc aaacaacacc
    22981 aaaattgacg gaagccctgc cttccagaaa gattcgtatc aggggcgcaa cttccatttt
    23041 ggcgtgcgcg aacatgcgat gggtgcggcg ttgaacggca tggcggtctt tggcggcgtg
    23101 attccctatg gaggcacctt ccttgtattt gccgattatg tgcgtccatc cgttcgtttg
    23161 gcggcgcttt cgcatattcc gtccattttt attttcaccc atgatagtgt cggtcttggt
    23221 gaggatggtc caacacatca acccattgag cacctgacct cgctgcgctt gatcccgaat
    23281 cttgtggtca ttcgccctgc agatgcaaat gagactgtcg ccgcctggaa ggttgccatt
    23341 gaaaggcgca acggcccaac cgtccttgcg ttgacccgac aaaatgtccc gactttggaa
    23401 acctctgcac aggttgaaaa aggtgcttac gttttgaagg attttggaaa tccagaaatg
    23461 attctgatgg cttcaggctc cgaggttggt ttggtccttg aggctgctca aaaacttgcg
    23521 gatgagggca aaggtgtgcg cgtggtttcc ttcccaagtt gggaactgtt cgagaaacag
    23581 gatgaggcgt atcgcgagtc ggtgctgccg aatgccatcc agaagagact cgctgtcgaa
    23641 gcaggatcag gcctcggctg ggaacgatac gcgaagtccg tcatcagcat tgaccgatac
    23701 ggcgcatctg ctccggcgaa ggtcatcttc gagaagcttg gttttactgt tgaaaatgtt
    23761 gttgcgaagg caaaggagtt gtagtaccga cttaagtcgg tgctacggaa aattttatga
    23821 aagtagcagt tggttgtgac cacggcggat ttccgctgaa agaagttgtg atcgaagcag
    23881 tgaaggctgc cgggcacgaa gtgatcgatg tcggcactta tagcgtggat gcagtggatt
    23941 tccctgattt cacaaaaaag gttggcgaaa aaattcaaag cggcgaagcc gaacgcggtg
    24001 tggtgatctg cggttcaggc atcggcgcgg cgattgcggc caataagatg aaaggcatct
    24061 acgcctcgat ctgtcacgat acctattctg cggcgcaggg tgttcagcat gatgcgatga
    24121 acgttctgtg cctcggcggg cgtgtgatcg gccctgagtt ggtaaaagtc atcgtgcccg
    24181 catttttagg cgcgaaatat cttggcgatg aaaaaggcgg cgaacgattt gcgaaacgtg
    24241 tgaataagat caaagagttg gaaggttaga acattctaac gttgaaacgt tccaagaggt
    24301 gacatgccaa cgatcagacg ctttgaagaa gtcgaagcat ggcaaacagc gcgggaattg
    24361 acgcggcttg tatactcgat tacagaagaa ggaaaatttg ctaaagactt cggattgaaa
    24421 gatcagattc gtcgggcatg tgtttcgatc atgagcaata ttgcggaagg cttcgaaagc
    24481 aaaacgcaac ccgcatttat ccgttatttg ggtattgcca aagcctctgc aggtgaagtc
    24541 cgcgctcagt tgtatgtggc aaatgatgtg aaatatttga gcgacgaaca atttgcacag
    24601 ctttttgaca ttgcagaaaa atcatcccgc cagctctcgc gatttatttc ataccttgaa
    24661 gcccaaccga acacttaccg cgttaaagac gaatctcccg aatacgaaac ctgatctcaa
    24721 cgttaaaacg ttctaacgtt caaactttct aactttctaa ctttcaaact ggaaccctaa
    24781 aatgaaccca atcccaaaac ttacatccct cggtcaatcc ctttggtacg acaacattca
    24841 gagaaaactt ctcgaaaacg gcgaactcaa ggcgatgatc gagcgcggtg atatccgcgg
    24901 cgtgacctcg aacccgacca tttttcagaa cgcgattgca aagacaaccg attacgatgc
    24961 cgcgttgatt ccgcttgcat gggcaggctg ggatgcggaa aggatcttct ggcaggttgc
    25021 tgttgaagat attcaggaag cctgcgacct ctttcgcccg ttgtacgatg aaacgcgcgg
    25081 cggcgatggg tatgtgagtc tcgaagtcag cccgtttttg gcgaacgata ccgaaggcac
    25141 gcagaagcag gcgaaggaat tgtgggaacg cgtcaaccgc ccgaacctga tggtaaaaat
    25201 ccctgccacc aaggcgggca ttcccgccat ccgcagcacg attgcggcag ggatcaatgt
    25261 caacgtcaca ttgatcttct ctcttgatcg ctatgccgct gtgatggatg cctatctggc
    25321 tggcctcgaa gatcgcatcg ccgcaaatga atcgattgaa atggtcgcgt cggttgcgtc
    25381 attcttcgtt tcacgcgttg acacaaaaat tgatccgcgt ttgcccgaag gttcacccct
    25441 gcgcggacgc gctgcgatcg caaatgcaaa acttgcctac gatgcttttg agtctgtttt
    25501 cacctcgccg cgtttcgcca ccctcaaagc ccgtttccgt gcgcgtgtgc agcgtccgct
    25561 gtgggcttcc acaggcacga aaaaccccgc ttactccgac acgctttatg tcgataccct
    25621 catcggccca gacaccgtca acacggtgcc gcccgccacg ctggatgcct tccgtgatca
    25681 cggcaaggcc agcctcacca tcacccgcgg acttgaagaa gcgcagttgt tttttgccga
    25741 actcaaagcc gcagggattt cgatgggctc cgttacgcag gaattggagg aggaaggtgt
    25801 caaggctttt gacgatgcct tcaagactct cttgcaagcc attgaggatc ggaggaagag
    25861 cgccgtttct tctcttggac ctttagctga ttctgtggcc aaacgtttga ccacgctgga
    25921 agaacactcg gtcgccgccc gcatctgggc acatgacccg acactttggg cgagtgaccc
    25981 cgaaggtcag gcagagatca agatccgcct cggctggctg cattccatcg aagatgcccg
    26041 cacacggttg gatgggtatc tctccttcgc aaaacagatt cacgaagaag gcattgaccg
    26101 tgtcctcgtc atcggcatgg gcggatcgtc actgacggct gaagtgctca gctcgttgat
    26161 ggctgcggcg aatgttgatg cgaaattatc ccttgccatt ctcgattcga ccgattctga
    26221 gcaggtggct ttggcagcga agaattatcc gccagagaaa tcgttgtaca ttctcgccag
    26281 caaatcaggc ggcaccgcag aattgctggc cgcgttcgat tatttctggg aactcagcaa
    26341 gggcaatggc tcgcgcttca tggtcaccac cgatgcgggt acctcgcttg aaaaactcgc
    26401 caaggatcgc gggttcagaa aagtattcaa cgccgatccc aatgtgggtg gacgtttctc
    26461 tgcgttgact gatttcggtc tggtgcctgc ggctttgctc ggcatggata tcgaaaagtt
    26521 gctggcttct gctgagaaga tcaaaaatgc ctcacacaag aacgtcagcg cggggtttgc
    26581 gcttggcgct ctactggctg agtctgctct cgcaggcaga gacaagctca cggtcctgag
    26641 tgatgcgcct gtttcagcgt tggcaggttg gatcgagcag gttattgccg agtccagtgg
    26701 caagcatgga aagggaatcc ttcccgtgcc gatggagcct ttgggttcac ccgatgtata
    26761 tggtgatgac cgcctgtttg tatatttgcg aaacaacggt gaattgagtg atggcattgc
    26821 cgcgcttaag aatgcaggtt tccccgtcgt cgaattgcca attgccaatc tctacgacgc
    26881 aggcgcggaa ttcttccgct gggagatcgc gatctcggtg gcctgccaca ttttgggaat
    26941 caacactttt gatcagcccg atgtgcagga tagcaagttg cgcacgatcg cgaagatcaa
    27001 agattatcag gccacaggca aactcgccga agcggatctg gtggacgtcg ccgatgcgaa
    27061 atctgcgttg gagaaattca tgactggggc gcaggcaggc gagttcttca ccatcaatgc
    27121 ctatctgccg cgcaacaaag agaccgagaa tgcgttgcaa gccttgcgcg ttgccattcg
    27181 tgcaaagacc aaatgcgctg tttcggctgg ctttggtccg cgcttccaac attccacggg
    27241 gcaattccac aagggcggcc ccaacaaggg acgttttatt caggtggtgt acgatgccca
    27301 aaatgacatg gacattccca atcagggcat gaccttcggc acgctgatcc gcgctcaggc
    27361 gctgggtgat tacgaggcgt tggtggccgc gggcagaaaa gtattgcgtg tgaagttgaa
    27421 ttcggtggag ga
//
LOCUS       DAOQLC010000056        27196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_582_length_27196_cov_22.324712, whole genome shotgun sequence.
ACCESSION   DAOQLC010000056 DAOQLC010000000
VERSION     DAOQLC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_582_length_27196_cov_22.324712"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..635)
                     /locus_tag="PL000_11290"
     CDS             complement(75..635)
                     /locus_tag="PL000_11290"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC89529.1"
                     /translation="MSQSKRSNFGINILRALALLTVIGITVFIYSIRERVDEFSHYGY
                     PGIFLITLMANATVILPAPGVAVVFAMGGVFNPFFVGLAAGTGGAIGELTGYMTGFSG
                     QAIIENTKIYDQISPWVQKYGVWAILILSAIPNPFFDLAGIAAGVAKIPLWKFLLFCW
                     IGQIVKMSLFAYAGAYSIDWIVGLYK"
     gene            complement(613..1035)
                     /locus_tag="PL000_11295"
     CDS             complement(613..1035)
                     /locus_tag="PL000_11295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC89530.1"
                     /translation="MVIELFADKTPVTVNSFVFLAREGFYDGVIFHRVISNFMAQGGD
                     PTGTGRGGPGYRFQDEFHPSLKHDKRGILSMANAGPGTNGSQFFITHGPTPHLDNRHS
                     VFGQVVDGLDVLMSIPERDPMNVNAPAVKIIRVTIEEI"
     gene            complement(1142..1771)
                     /gene="udk"
                     /locus_tag="PL000_11300"
     CDS             complement(1142..1771)
                     /gene="udk"
                     /locus_tag="PL000_11300"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNC89531.1"
                     /translation="MSHEVPLVIGIAGGSGSGKTTVAQEILKRVGADRIAYLQHDSYY
                     KDLTKLPPELRTDGKNFDHPHSLETELLIKHVIALKNREAVEVPIYDFTTDSRMAKTF
                     TVKPQDVILVEGILIFVEAKLRKLFDVKIFVDTDSDLRFIRRLKRDISERGRTTESVI
                     AQYMATARPMHLEFVEPSKRYADVIIPEGGHNVAALDMVVARIESLLKK"
     gene            complement(1845..2048)
                     /locus_tag="PL000_11305"
     CDS             complement(1845..2048)
                     /locus_tag="PL000_11305"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC89532.1"
                     /translation="MVAGGEVKLPLTSFDRSLRLGSGLLFVMVGISTNNWIVGVLGGI
                     VAFMGVYDRCPIWQALTGLFKRS"
     gene            complement(2061..2357)
                     /gene="trxA"
                     /locus_tag="PL000_11310"
                     /pseudo
     CDS             complement(2061..2357)
                     /gene="trxA"
                     /locus_tag="PL000_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(2400..2597)
                     /locus_tag="PL000_11315"
     CDS             complement(2400..2597)
                     /locus_tag="PL000_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC89533.1"
                     /translation="MKRNMSNIDRIVRVVLAALFAYLYFSGIVTGTLGLVLVVLGGVF
                     LLTAIVAFCPLYAPFKFSTYK"
     gene            complement(2671..3345)
                     /locus_tag="PL000_11320"
     CDS             complement(2671..3345)
                     /locus_tag="PL000_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC89534.1"
                     /translation="MIDVTQFERISHALPILQQADEAFKKEFQRAAFFARIPSGHDVF
                     LEGDRVEAIALLLSGVVRVYKIGETGREITLYRFGNGSSCILSANAILSQKTFPAIAT
                     VEEDAEAVMIPADTFREWVHRYNLWSDFVFDLLSQRLSTMMAVVDEVAFQRMDRRVAA
                     LILERAQKQNPLPITHQEIASELGSSREVISRLLEDFVNDGSLRSTRGKVEVLDFELL
                     ESRLAM"
     gene            complement(3412..4263)
                     /locus_tag="PL000_11325"
     CDS             complement(3412..4263)
                     /locus_tag="PL000_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC89535.1"
                     /translation="MPKFALVTDSTAYIPTELVKKHNITVVPLILVWGDEMFADGVDI
                     QPEQFYSRLKTAKVMPSTSQATPATMQAIFQPLVDQGMEVLAMFISSKLSGTMQSAIQ
                     AKEMMGSAGEKVTLVDSQCTSMALGFQVLAAARAMDAGASLSECAAIAEKAHEKAGLF
                     FAVDTLEFLHRGGRIGSAQRFIGSALNLKPILALKEGRVEGVERIRTKGKAQDRILEL
                     VAEQVKGKANIRLAVVHANAPEDAKTLLDRAKQSINPSEAFVTDLSPVVGTHAGPGTV
                     GLAFMTD"
     gene            4456..5529
                     /locus_tag="PL000_11330"
     CDS             4456..5529
                     /locus_tag="PL000_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNC89536.1"
                     /translation="MAFNPINWLLGLFSLDIAIDLGTANTLVHVRGKGIVINEPSWVT
                     VDKKLRQPVAIGLEAKEMVGRTPGNVMVVRPIRDGVIAEFDVTQVMLEYFIGKVHEQS
                     MVPLPRPRVIVGLPTGVTEVEKRAVYDAVMASGARQAMLIEEPIAAALGAGLPVGEIR
                     GSMVVDIGGGTTEVAVMSMSGVVSSRSLRVAGDEMDQDIVQYLRNKYNLLIGEGIAEQ
                     VKWQIGSAYPLQPEKTMEVRGRNLVTGLPETVEVSSVEIREALMGSVQVIIDTVRDAL
                     DEIPPEIVADLMDIGICLAGGGALLQGLAERLTDELKLRVWVAEDSLTCVVRGAAMIF
                     EDLSTFNRYLVGLERGSTRHITG"
     gene            5568..6404
                     /gene="mreC"
                     /locus_tag="PL000_11335"
     CDS             5568..6404
                     /gene="mreC"
                     /locus_tag="PL000_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC89537.1"
                     /translation="MNSRSLQTTIIFLVIGGILALAFGGFFGPASRQFSSVLVQFQTW
                     VSSRYLGVQDFFTAPRDIVSLRARNAELESQVSQLQAQVIELQQRVNETEILAALVDF
                     SRSNPESTYKAAAVIGRDPSPFLHYIIINRGSNDDIRRGMPVVTNQGLVGRVDAVIAD
                     AARVQLITDPASAVNTYLQNADTTAVALGSVTGDVSLDMISQDAIVQPGDLILTSGLG
                     GGYPADIPVGQVITLRSLEYELFQQATLQPAVDFTRLSIVLVITNFRPVDISPLEPLP
                     SP"
     gene            6423..6923
                     /locus_tag="PL000_11340"
     CDS             6423..6923
                     /locus_tag="PL000_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89538.1"
                     /translation="MRNIVAFPLLVLAVILQSSIVSQVRLLHGYADLILIMLAAWALQ
                     ERVRSSWHWALVACILLGIISKLPWAVSTIGYLAVVFTAQVLQRRVWQAPLLAMFSVT
                     LIGTIFMHLLSYTVLSLSGTPFSFSAVAGSITLPSLLLNIFFSIPIYALMRDLARWVY
                     PSEVYE"
     gene            6920..9124
                     /gene="mrdA"
                     /locus_tag="PL000_11345"
     CDS             6920..9124
                     /gene="mrdA"
                     /locus_tag="PL000_11345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC89539.1"
                     /translation="MSSNSVPRSVRFEAWRLVTVYVVVVLVFVAFLVRLLRLQVIEGA
                     NWKTSAVDNYTFEVSVPAPRGIIYDRNGYILARNVASYNIIITPANLPDDDSDIQAIY
                     RDLSDLLDVPVGGRADDEALLEEAKLFGPCVPGPGITQLVALQDSLAPYSPVKIKCNV
                     TVDIARIVEEKSVDWPGVTVEIEPIRDYPTGSLTSALVGFLGPIPASLEEEFRAQGFI
                     PNRDKIGYAGVEDSLQEILAGRNGLRVVQVDVAGQELRNLEPPIPAVPGNNVTLTIDT
                     RLQAVAEASLIQEINFWNDYFDTIRISSGVVIAMNPKTGEILAMVSYPTYENNRFARF
                     IPAYYYEQLSQDPRHPLLNNAISAEFPPGSVFKLSTATGSFNEGVIGPDDIVDAPGKL
                     ELCEKFNPNDPCTGLNTRPFVDHIFEKKPEGFGPISFYQCIAYSSNVCFYKLGGGYQD
                     EIEQGLGIERLQQYARALGYDQPSGIELKGEQDGLIPDPKWKRINTGENWSTGDTYIA
                     SVGQGYVLATPLQILMSGATIANNGKLMQPTIIREVTDGDGKPVDIWFNPDPEVFTAT
                     DFEVPGSYQISPFTPNLKWDVTVTPLIQSYSCDAGYCDLNENDLKTIRPEAVAAVRAG
                     TRLAVTDPFFGTLYDVFNDFPIAVAGKTGTAEYCDDVARKADQCDFGKWPTHSWTLAY
                     APYDDPEIIVVAFAYHGGEGASVAAPIVSRVIKAYFEFKALDIAQGNPALGQ"
     gene            9219..9899
                     /gene="minC"
                     /locus_tag="PL000_11350"
     CDS             9219..9899
                     /gene="minC"
                     /locus_tag="PL000_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNC89540.1"
                     /translation="MEQITSLVQIKGIRDGLLATFSEAPWEDQCLALLTQIDERPAFF
                     QGARLAMDVGTQVLKVNDLVDLRDRLSERNIALWAVISESPTTEQTSQLLGLATRISK
                     PRPEEQRQVADSISDDRALFINKTLRSGTRVEFPGNIVIVGDVNPGAEIVAEGNVVVW
                     GRVRGMIHAGAKGDRSAYICALDLSANQLRIADEVSAMLKPQKDPKPEIATINGEGRL
                     QAELWHVG"
     gene            9922..10719
                     /gene="minD"
                     /locus_tag="PL000_11355"
     CDS             9922..10719
                     /gene="minD"
                     /locus_tag="PL000_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNC89541.1"
                     /translation="MTAQVITVTSGKGGVGKTTAVANLATALATDGRKVVCIDGDIGL
                     RNLDVIMGLENRIVYDIVDVIEGRCKLKQAMIRDKHFPDMYLIPAAQTRDKNAVSPSD
                     MVRICNDLRPDTDFIIIDSPAGIERGFRNAIAAADRVLVVTNPEVSAVRDADRVVGIL
                     EAEEKGSPALILNRLNPTLVRNNDMLSPEDVLDLLGIQLIGIVPEDESVIIGSNRGAP
                     VVTDTKSRAGQAFRNIAKRIQGQNVPFMDLEQQGGLWGAIQKLTGRK"
     gene            10721..10972
                     /gene="minE"
                     /locus_tag="PL000_11360"
     CDS             10721..10972
                     /gene="minE"
                     /locus_tag="PL000_11360"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNC89542.1"
                     /translation="MNFFTRKRSAESAKERLQLVLIHDRTDLTPGQLEELKDDLLKAI
                     SRYIDIDPEAVQIGLERDGRSQRLVADIPLRSASRHRAG"
     gene            11054..12181
                     /locus_tag="PL000_11365"
     CDS             11054..12181
                     /locus_tag="PL000_11365"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNC89543.1"
                     /translation="MNREFSWRNFDFILLGAIVLASSFGTAMIRSAVAGNEVLQPLII
                     RQVYFAILGVVLIFLVGSIDYRYWLALYRPIYFGMIAFLVTLTGFGQSAFGAQRWFQV
                     GVLFLQPTEFAKIVAIIVLGRYFEVAQHQPRDLKWAFGAIAWAAGIIIMILLQPNLSN
                     VLLLSVILAVMLWMNGVQVKHVFSAAAIGAAAALSLIVLSVLGISIPGLQPYQQERIV
                     NFVVPDPNDTYGNRYNVQQALIAVGAGGLFGEGYGHGTQTQLRFLKVRHTDFIFAASS
                     EEFGMVGGALIIITLGIIVWRCIRAAQKSRDVAGATIAIGVATLIFFQAAVNIGMNLN
                     LLPVSGLPLPFISYGGSGLTALMLGIGLVESVVMRHKQMEF"
     gene            12265..13746
                     /gene="gltX"
                     /locus_tag="PL000_11370"
     CDS             12265..13746
                     /gene="gltX"
                     /locus_tag="PL000_11370"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC89544.1"
                     /translation="MTTKHARTRFAPSPTGHLHLGGARTALYAYLLAKKTGGQFLLRI
                     EDTDIKRTVPGAEQEIMDGLRWLGLNYDEGPDIGGKYGPYRQTERREIYQAHAKTLVE
                     NGHAYPCFCTPEHLDKVRQEQMKRKENPRYDGTCRVLSADEAAKRIANGEPYTIRFKV
                     PYEGSTVAHDHLRGDITIENKQLNDQVILKTDGMPTYHLAAMVDDHEMQITHVIRGSE
                     WLGTFPLHVNIVRAFGWEEPVWAHLSVFLKPSGKGKMSKRDAPDAMKDGYSVFIKDMQ
                     DLGFTPEGVLNWCALMGWGVAEDDVMTVDQMVDRFTIDSLTPSPAAVNFQRLDHFNAT
                     HIRLFTTEDLAARIKPYFTREGLKVDDGILLKIVPLIRERLTTLDDCLSFGSFFFKED
                     VSPAPADLIAKGLDAKQSAQIAQRAYEILVAQPDISHERCEPPLRAYVEESGLNANQV
                     FGILRVATTGQKVSPPLFESMEIIGREKCLARIKSAIELLEKM"
     gene            complement(13799..14752)
                     /locus_tag="PL000_11375"
     CDS             complement(13799..14752)
                     /locus_tag="PL000_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89545.1"
                     /translation="MESSATSVVPEKNTRRKWLELLLDNPVITKELKGRMRGRQGFIL
                     LSAYLALIGFFIGLVYLFLSVDGSLSRNDPGYLQIVGKIIFSTVVLLELLMVSFIGPA
                     LTSGAISSERERQTFDLLRTSLLSGRALVFGKLSSAVVFLLLLIFTAIPIQSMSFFLG
                     GVGMAEMVISIVMLVVSIFFFSALGLFFSSFTKRTLVSTVAAYASIMLSFIVMVLLFF
                     FILVFAGVASSTNTFLSGISEEVLGISVWYLFSTNSFFAAVASEAILVSDQSIFFTTN
                     GMLGTTSLPLPSPWIIYTVFHVILTILLVMASIYFVNRPDK"
     gene            complement(14762..15709)
                     /locus_tag="PL000_11380"
     CDS             complement(14762..15709)
                     /locus_tag="PL000_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89546.1"
                     /translation="MSPIIEIKDLCKHFGKRFAVNNISFNVESGEIFGLVGPNGAGKT
                     TTMRMLVTLLRPDKGEIFVGGHSIRKNPNDVRRMIGFMPDSFGVYGDMTVHEYLDFFG
                     ACYKVPPNQRLRLINDLLELVDLGHRRDDMVDTLSTGLKQRLGLARVLIHDPNILVLD
                     EPASGLDPRARVEIRELLLEIARLGKTIIFSSHILADVSELCTRVGIIEDGKLAAIGT
                     LQQLSERVMPHRQLRISLLDRAQEAQINLQSRAGVNEARVLENTERPTLEVDFIGDDE
                     AIHQLLAGLLAQGYPVVHFSEETKNLEEVFMRTTRGIVS"
     gene            complement(15706..18087)
                     /locus_tag="PL000_11385"
     CDS             complement(15706..18087)
                     /locus_tag="PL000_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89547.1"
                     /translation="MKLPYRSAIYKQTAFLAMIFAAILFTSWTSTTAAAQTQGEITIK
                     AQSGLDNFCKNGTWLPVKVMVENNGKDVDARVQASYKNDMNGNAVYGMDISLPATSRK
                     EFFIYVFLDGTARNFNVSVLDGNKTLAKTALNIACINDQTLLFGLVTDDPSVFAGLNT
                     ISLSGVNAQSAQLKVEDLPDQAQGWEIMDALVISNVDTGKFSAEQKQAMEMWLARGGR
                     LFVTGGTNWQPTAAGLENILPIKLTSTKEVTNLSAISKYSKFPLLTEEQSAVLATGQL
                     LEGATTLVDQSGIPVLVEKQVGFGKVYFFAADPGLEPLNNWVGMLAIYEHLLGFRSPK
                     PVWANGYWDAYQINDALSSMPELALPSFVYICCWLGLYVFVVGPLNYFVLRRIKRPEL
                     AWVSIPILVILFTMLAYFSGYAYRGTRPVLNRLMMAQGWEGVSQSQVNAVIGVYSPSR
                     TTYTVETQEQFMLRPTPITSGNLQGNDDWTSIKTQDGTSMPDIRVEIGGMQVVGATGY
                     LPALTVQSDLTITLGSHVPELSGTITNTGDYTIKDAVLVTASQWSPLGDIAPGQTKKV
                     SQILVNNSNTSVTSQYTIMTTLNIDTYDYYSDVNPEKKRQAAFFQANTNTSNGSIHVN
                     SGIYLMGWVNDEIATPTGLQSQPQEEIDTMLFFERLTPSIKTEAGSLMLTSSIFSWDS
                     SLGDTITTPYYNMSNNGYTLRYQPNQTIRFSKVNTFELNIESTTTPDKVIVSLWNFET
                     QTWDLIPMTFYTIDIPNPQQYIGMDGEVRLNLKGDPNDYVEITAINFTMTVQP"
     gene            18173..19462
                     /locus_tag="PL000_11390"
     CDS             18173..19462
                     /locus_tag="PL000_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNC89548.1"
                     /translation="MPAIEKFQTNSLRSQKYDAEVTRILAAMFQSVEPGAAVKRHLRE
                     NPLPAANRTFVFGLGKAACAMASALTDEIHPIDSLLITKHASPLDLKPATVIEGNHPV
                     PGSLSLAAGAAALKFLSQLTADDLLICLISGGGSALMAAPRIPLVDLQTLTSALLACG
                     ARIDEINILRRHLDQLKGGGIVRHANGARVVSFLLSDVVGDPLEAIASGPTAPDPTTC
                     ADALAVIEKYDLKDKIPSSILRALTETLKPADPLFANVQNTIIASNAIALRTAQVCAQ
                     QEGFQTRVWRSDMQGEAAVLGREMVLELSEMLKNTPRPFCLLAGGEATVTLNSVKNGK
                     GGRNQEFALGAVDELAGMADVLLISLATDGEDGPTDAAGAMVTGASAQRAESLGVSVA
                     DHLSGHDAYPFFEMLGDLVRTGPSGTNVNDIVLCFAF"
     gene            complement(19469..20419)
                     /locus_tag="PL000_11395"
     CDS             complement(19469..20419)
                     /locus_tag="PL000_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC89549.1"
                     /translation="MTKSAFRCLFALALLLVACTSPNPSPTTNPPATNTPSQEQHFIV
                     FGDISDDPAEVIEGTQPIADYVAAKLAAYGITGGHVKVVASTEEMIELLKRGEVDVYF
                     DSTYPATLISDASGAQIILRRWRFGVETYNSVIFASKASGITSIEGLKGKMIAMDTPY
                     STSGFMLPAVYLTENGLKLIGKSESTATVDSNEVGFAFAYDDENILQWVLSGHTPAGV
                     TDDYNFDHAFPPEATAQLVELARTESTPRQVGLARAGLEPELLQAIVQILTTMHEDPN
                     SQTALEKFQTTRFDEFPEGIEAATGRMREMMEIVKDIPLP"
     gene            complement(20451..21059)
                     /locus_tag="PL000_11400"
     CDS             complement(20451..21059)
                     /locus_tag="PL000_11400"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNC89550.1"
                     /translation="MTETLLLLFRGFLIGISIAAPVGPIGVLCIRRTLTEGRFKGFLS
                     GMGAASADMVYGAVAAFGLTMITDVLVENKFWFRVIGGCFLIYLGIKTFLEKPADHAA
                     QVKQSGYFGAYLSTFFLTITNPMTILSFAAIFAGTMNIGATSSPMVLVAGVFAGSASW
                     WLALSFGVGLMRDRLTPVHMMWINRLSGIIITTFGAFALAQL"
     gene            complement(21127..22077)
                     /gene="secF"
                     /locus_tag="PL000_11405"
     CDS             complement(21127..22077)
                     /gene="secF"
                     /locus_tag="PL000_11405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNC89551.1"
                     /translation="MNIIKNRYIYFLISLLVIIPGIVFMGINWQQTKAGPLQLGIDFR
                     GGSLLEVQFEGERPTAEEISALYSDFSTEEQPIADPVVQSLGESSISIRSKAMTDETK
                     GLIVAEMESRFGSKVTVENFTSVSASVGAEVTQAAGVAILFAAIAILIYIWWAFRSVE
                     HAYRYGTAAILAMLHDVLVVLGVEAILGYYLGWEADALFLTALLTVIGFSVHDTIVVF
                     DRVRENSLLLRKIEYEKMVNHSIVQTLDRSITTQLTVMFTLFALVLFGGDSIRHFVVI
                     LLIGIFSGTYSSIFNAAPILVVWENREWTTWFKPKEETAI"
     gene            complement(22087..23442)
                     /gene="secD"
                     /locus_tag="PL000_11410"
     CDS             complement(22087..23442)
                     /gene="secD"
                     /locus_tag="PL000_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC89552.1"
                     /translation="MRNRNSWLIITLLLVAFSLWIDLSKDIVVYNPFNNTPLVERNVD
                     VKLGLDLRGGLQALLEADVPAETTVSAEELQNATNILQNRANALGVSEITMVTAGERR
                     IVAEFPGLTNPEEMVAALKQTALLEFVDMGSAPVPDGTTILTDFGLETPGQTTNENGE
                     TIYHTVMTGAGLDSAVVQRDPSSGQIAIAFTLDSDAADVFSEYTSSHVGQYLAIVLDK
                     RVISAPRINQAITDGQGQISGGFTQESAQTLAVQLRSGALPIPIKVVQSRTIGATLGE
                     ESIRRSVQAGIIGMIVVILFMAFNYRLPGVIADLALVCYTLFSLMLFKLIPVTLTLPG
                     IAGFILSLGMAVDANVLIFERLKEELRSGRNLRQAIDLAWSRAWPSIRDSNTSTLITC
                     AILFIFGNTFGASIVKGFSVTLSLGVVVSLFTAIIVTRTFLHSVLDSVKTTEHPRWFG
                     L"
     gene            complement(23513..24994)
                     /locus_tag="PL000_11415"
     CDS             complement(23513..24994)
                     /locus_tag="PL000_11415"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNC89553.1"
                     /translation="MKKIFLLLMLALLLLPACLPARQAAESTPQQNPNPTADPADRSI
                     YKDGLTPAYQSVLNELPTASLYEIHFTIEDDLYHVKGHETVIYTNAEDSALNEVKFRL
                     FPNILGGEMHVDEVDAGGTIVVPNYTLDDSLLTVPLKQPLQPKESITLSMGFTVTVPQ
                     SVELNYGVQAYFNDVLTLAHAYPMIAVYDDEGWNAEIPPQSGDVTYADMSFFNVTVDA
                     PKDLVLVSVGREILRQAERNGNRQTVQYVAGPVRDYYLAASPNYEVVTKESNGVTLRF
                     YAPADQRSGAERTLEIAAKSIEVYENRYAAYPYTELDFVSTPTLALGIEYPGMIAIAD
                     RIVSPYHEYLEGTVAHEVGHQWFYNLVGNDQLDDPWLDESLTQFATLQYFSDQYGQSG
                     YEGFRFSLEGRWARTNNAAIPVGMPVRDYKDIEYGSIVYGRGGLFFEALRDEMGSENF
                     DQFMKAYVNENAWDIATPEKMKQLAEQNCTCDLTDLFKSWIYP"
     gene            complement(24991..25356)
                     /locus_tag="PL000_11420"
     CDS             complement(24991..25356)
                     /locus_tag="PL000_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89554.1"
                     /translation="MKDEDSTPLIGIVGPCGSGKSTLMAGLETHGYRCRHIAQEHSYV
                     KHMWQVITNPDILIFLHSSFENSTQRRKLNWSLADYEEQQRRLSHARTHAHIIIDTDL
                     LKPDDVLARALTFLKEFTQ"
     gene            complement(25346..26419)
                     /locus_tag="PL000_11425"
     CDS             complement(25346..26419)
                     /locus_tag="PL000_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC89555.1"
                     /translation="MASTAHSHHSGSRKHRWRHFQASIRDTMILLGEFRSAILWFSVA
                     ILGGGILYDFLSRLLNEPVGSLAESIYTVLTITFLQPPNRSFPTHIVLQLFHFLMPII
                     GIIMLAQGLADFGSLLFNRRSRNKEWEMAVASTLKNHIILVGLGHLGYRVALKLHEMG
                     EQIAAIEFNADADTLSVVRGLGIPVIHEDATRPSSLEAANVKSARTIIMASQNDAMNL
                     QIALKARTLNKNIKVVVRIFDEDFAHSLQDQFGFVALSATEMAAPVFAAAASGVDITN
                     PISVEGQLLSLARLTVSEKAPFANKTIGYVEDNYRLNIVLLRHDHQSEMHPTDKSPLH
                     AGDTIAVLGVPEQLSHLLHDNEG"
     gene            complement(26412..>27196)
                     /locus_tag="PL000_11430"
     CDS             complement(26412..>27196)
                     /locus_tag="PL000_11430"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNC89556.1"
                     /translation="LSLVHPNSKNPITCLKQGTDSKIILVEIQIAVAKINKYAVSESG
                     DTLEVVERPTGGLSVVLADGQTSGRGAKAVSMMVVRKVIGLIAEGVRDGAAARAASDA
                     LFAEKQGKVISTLNIASVDLHSNTIVLTRNNPSPVFICRGEQVDAHTEESEPLGTSRN
                     VRPVITEVAVEPELAIVIFTDGIVHAGKRRGQPMDVAETLRALIEDQDPSPQQLADSL
                     LAHAVQLDDGRPADDISVLVLKVSPRAGDHVRRMNVRLPFNG"
BASE COUNT         6242 a         6636 c         7468 g         6850 t
ORIGIN      
        1 ttgtggtgaa aaaatggact tatgcaagag ttctattcaa tatcatctga aagtgcggga
       61 tttatcccgc acttttattt atacaagccc acaatccagt caatggaata tgcgcctgcg
      121 taggcaaaca gactcatttt gacgatttga ccgatccagc aaaagagcaa aaacttccac
      181 aatggaattt ttgccacgcc agctgcaata ccagccaggt caaaaaacgg gttcggaatt
      241 gcagaaagaa tcaaaatcgc ccagacgcca tatttctgca cccagggcga aatctgatca
      301 tagatctttg tgttttcaat gattgcctga ccgctgaacc ctgtcatgta accggtcaat
      361 tcaccgatgg cgccgcctgt tcctgcggca agccctacaa agaacgggtt gaaaacaccc
      421 cccattgcaa atactactgc cactccgggg gctggcagaa tcactgtggc atttgccatc
      481 aaggtgatga ggaagatccc cggataccca tagtgtgaaa attcatccac gcgctcgcgg
      541 atgctgtaaa tgaacacagt gattccgatc actgtcagca gcgcaagcgc gcggagaata
      601 ttgatgccga agttagatct cttcgattgt gacacggatg atcttgaccg cgggggcgtt
      661 tacattcatg gggtcgcgtt cagggatgga catcaacaca tccaacccat ccacaacctg
      721 cccaaagacc gaatgacggt tatccagatg cggagtgggg ccgtgggtga tgaagaactg
      781 cgagccattg gttccaggcc cggcgttcgc catcgaaagg attccgcgtt tgtcatgctt
      841 caggctgggg tggaattcat cttgaaagcg gtaaccgggg ccgccacggc cggtaccggt
      901 cgggtcgccg ccttgagcca tgaaatttga gatcacgcgg tgaaagatga caccgtcata
      961 aaatccctcg cgcgcgagga acacaaagct attcacagtg acgggggtct tgtctgcaaa
     1021 cagttcgatc accatggtgc ctttatcggt ttccatgtgt gccttgtact gtttcttcgg
     1081 gtcgatctgc attgcgggtg gggtgctcca ttgttttgcc attccaaact ctcctttgaa
     1141 tttatttttt gagcaacgat tcaattctcg ccacaaccat gtcaagcgct gcgacgttat
     1201 gtccgccttc ggggatgatc acatccgcgt accgtttcga aggttcgacg aattccagat
     1261 gcatgggtcg tgctgttgcc atatactgtg caatgaccga ttcggttgtg cgcccgcgtt
     1321 ctgaaatatc ccgtttgagg cggcggatga aacgaaggtc agaatcggta tccacgaata
     1381 ttttcacatc gaacagcttc cgcagttttg cctctacaaa gatgagaatc ccctctacga
     1441 ggatcacatc ctgcggtttc accgtgaaag tcttcgccat tcggctgtct gtggtgaaat
     1501 catagatggg cacttccacc gcctcgcgat ttttgagcgc aattacatgc ttgatcagca
     1561 actcggtttc aagcgaatgg ggatggtcga aattctttcc gtcggtgcgt agttcgggtg
     1621 gtaacttggt caggtctttg tagtacgagt cgtgttgcag gtaggcgatt ctgtctgctc
     1681 caacccgttt gaggatttcc tgagcgacgg tggttttccc cgaccccgat ccgcctgcga
     1741 tgccgatgac tagtgggact tcatgactca tggcggcatt atagcccagg aatgaaaaaa
     1801 cggctccggg ccaacccgaa gcctgtttcg ctaaagaaag atatttagct gcgtttgaac
     1861 aaacctgtca atgcctgcca gatcgggcag cgatcataca cccccataaa cgccacaatt
     1921 ccgccaagga cgccaacgat ccaattattg gtggaaatac ccaccatcac aaacaacagg
     1981 ccagatccca gcctcaaaga acggtcgaac gaggtgagtg gtaattttac ttccccgcct
     2041 gcgaccatcg cttcgaacaa ggtgcggtac ctggcttcat tttgcgcgcc agtgacacgc
     2101 cccacttctt tttcgttgcg gaacatgagc acggtgggaa ttccaaacac gcgatactgt
     2161 tcaatgattt cgcgcgcttc atcggcattg actggcatga actcaaccct gtcgccatat
     2221 tccttggcga gtttttcaag gatcggtttg gtcatcatgc atggtccgca ccacggcgcc
     2281 cagaaatcca caatgacggg tttccccgcc ttggaaagtt tttgttgaaa ttccgtttta
     2341 ttcataagag taaggacaag gcgcggagcc cattttgttg ctccgcgcct tgttttagtt
     2401 tatttgtagg tgctaaattt gaagggcgca tacagcgggc agaaagccac gattgcggtc
     2461 agcaggaaaa ccccgccgag caccacaagc acgagaccga gggttccggt gacgattccg
     2521 ctaaagtaca gataagcgaa caaagcggcg agtacgacac ggacgatgcg atcgatattg
     2581 gacatattgc gtttcatgat aaatctcctt tggttgtttg aagtgatgcc cccagtatag
     2641 gagatggccg caaatctgtc ggtgacaatg tcacatagcc aggcgggatt ccaacaactc
     2701 gaaatcgagc acttcgactt ttccgcgtgt ggagcggagg cttccatcgt tgacaaaatc
     2761 ctccagcaga cggctaatga cctcgcgcga actgcccaat tctgaagcga tttcctgatg
     2821 ggtgatcggc agtggattct gtttttgtgc ccgctccaga atcaacgcgg ccacgcggcg
     2881 gtccatgcgt tggaaggcca cttcatccac cactgccatc atggtggaaa gccgctgtga
     2941 aagcaggtcg aagacaaaat cgctccatag attgtaacga tgcacccatt cgcggaaggt
     3001 atctgcagga atcatcacgg cttcagcatc ctcctccacc gtggcaatgg cagggaaggt
     3061 cttctggctg aggattgcgt ttgctgaaag aatacaggat gacccattgc caaaccgata
     3121 caaggtgatt tcccgtccag tttcaccgat cttgtagacc cgaaccacac cggagagcag
     3181 tagggcaatg gcttcaaccc gatctccttc aagaaaaaca tcgtgtccgg aagggatgcg
     3241 cgcaaaaaac gctgcacgct gaaattcttt tttgaaggct tcatccgctt gttgaaggat
     3301 cgggagggcg tgtgaaatac gttcgaattg tgttacgtca atcatatcta ttctatttga
     3361 gagatgagag cgcttttata gcgctctcat gttttagttt gcaattgttg cttaatcggt
     3421 catgaaggca agaccgactg tgcccggtcc tgcatgggtg ccaaccaccg ggctcaagtc
     3481 agttacaaaa gcttcggagg ggtttatgga ttgcttcgca cgatccagca aagttttggc
     3541 atcttcaggt gcattggcat ggaccacggc cagacggata tttgctttac ctttcacctg
     3601 ttccgcgacc aactcaagaa tacgatcctg agccttgccc ttggttcgaa ttcgctccac
     3661 gccttccacg cggccctctt tcaatgcgag gatcggtttc agattcagcg ctgagccgat
     3721 gaagcgctga gcactgccaa tacgtccgcc gcgatgcagg aattcgaggg tatccacagc
     3781 gaagaagagg cctgcttttt catgtgcttt ttctgcgatg gcggcacatt ccgaaaggct
     3841 ggcaccagca tccatggcgc gtgcagcagc gagcacctga aagccgagag ccattgaagt
     3901 acattggcta tccaccaaag tgactttttc gcctgcactt cccatcattt ccttggcctg
     3961 gatggcagat tgcatggttc cagaaagttt tgaagaaatg aacatcgcca aaacttccat
     4021 accctggtct acaagcggtt ggaaaatagc ttgcatggtg gctggtgttg cctgtgaggt
     4081 ggaaggcata accttggctg tttttaagcg gctatagaat tgctctggtt gaatatcaac
     4141 cccgtctgcg aacatttcat caccccaaac caggatcaaa gggacaacgg tgatattatg
     4201 ttttttaacc agctcagtgg gaatataagc ggtactgtca gtgacaagtg cgaatttggg
     4261 catgactttt ctccaaacaa tggaatatgg tgttgccatt gtatcccaac ggggagatta
     4321 agtcagggga tgttgaagca gatgtcgggt ggtggggagg cgggttttca tcctcataat
     4381 cttgcttttg gcgggcatgg tataatagta ctatctagtc cgtttttgaa tgaaatttca
     4441 ctttaggagc ctattttggc cttcaaccca attaactggc tgttaggcct tttttctctg
     4501 gatattgcca tagacctcgg cactgctaac actcttgttc atgttcgcgg caagggtatt
     4561 gtcatcaatg aaccctcttg ggtgacggtt gacaagaagt tgcgccagcc tgtggcaatt
     4621 ggtcttgagg ccaaggaaat ggttggccgt accccaggca atgtgatggt ggtgcgcccg
     4681 atccgcgatg gggtgattgc ggagtttgat gttacccagg tgatgcttga gtatttcatt
     4741 ggcaaagttc atgagcagag catggttccg ttgccgcgcc cgcgtgtgat cgtgggtttg
     4801 cctacaggcg taaccgaagt ggagaagcgt gccgtttatg atgcggtgat ggcttcgggg
     4861 gcacgtcagg ctatgttgat cgaagagccg atcgcggctg cactgggagc tggtctgcca
     4921 gtcggtgaga ttcgcggctc gatggtggtg gatattggcg gcggtacgac cgaagtggcg
     4981 gtcatgtcca tgagcggtgt ggtctcttcg cgttcgctgc gtgtggcagg cgatgaaatg
     5041 gatcaggata tcgttcaata tcttcgtaac aaatacaatt tgctgatcgg tgaaggcatt
     5101 gccgagcagg ttaaatggca gatcggttct gcatatccgc ttcaacctga aaaaacaatg
     5161 gaagtgcgcg gcaggaacct tgtgacgggg ctgcctgaaa ctgtcgaagt ctcttctgtt
     5221 gaaattcgtg aagctttgat gggttctgtg caggtcatca ttgatacggt ccgcgatgcg
     5281 cttgatgaaa ttccgcccga aattgtggcc gatttgatgg acattggtat ctgccttgcg
     5341 ggtggcggcg ctctcttgca aggtctggca gaacgcttga ccgatgaatt gaaattgcgt
     5401 gtgtgggtgg ctgaagactc gctgacttgt gtggtacgcg gtgcggccat gatctttgaa
     5461 gacctttcga ccttcaatcg ctatttggtt ggtttggaac gcggcagcac acgccacatt
     5521 accggttaat cgctcccccg gcattgcatg cctgaggaaa ttaaaatatg aactcccgtt
     5581 ctttacaaac gacgattatc tttcttgtca taggtgggat tttagcgttg gcatttggcg
     5641 gtttcttcgg acccgcctcc cgccaattta gctccgtgct ggttcagttt caaacctggg
     5701 tttcctcgcg ttatcttggt gtgcaggatt ttttcactgc gccgcgtgat attgtttcac
     5761 tgcgtgcccg caacgccgag ttggaatcac aggtttcgca gctgcaagcg caggtcattg
     5821 aattgcaaca acgtgtaaat gaaacagaga tcctcgccgc attggtggat ttttcgcgtt
     5881 cgaaccccga aagtacatat aaagctgctg ccgtcattgg ccgtgaccca agccctttcc
     5941 tgcattacat tatcatcaac cgcggttcca atgatgatat tcgccgtggt atgcctgtgg
     6001 taaccaatca gggattggtg ggccgtgtgg atgccgtcat tgcggatgcg gcccgtgtcc
     6061 aactgatcac cgaccctgct tcggcagtga acacctattt gcaaaatgcg gatacaactg
     6121 cagtggccct gggttcagtg acaggcgatg tttcactgga tatgatctcg caggatgcga
     6181 tcgtacaacc cggcgatctt atcctgacct cgggcctggg gggcggttat cctgcggata
     6241 ttcccgtcgg gcaggtgatt acgctgcgca gccttgaata tgaactcttt cagcaggcca
     6301 ccctgcagcc cgcagtggat ttcacccgcc tgtccattgt tctggtgatc accaactttc
     6361 gccctgtgga tatttctcca cttgagccat tgccttcccc gtaaactttt aaacccaatc
     6421 acatgcgaaa tattgttgca ttccctttgc tcgtgctggc agtgattctt caatcctcca
     6481 ttgtcagtca ggtccgactg cttcatggtt acgcagacct gattctgatc atgctggctg
     6541 cctgggcttt acaggagcgg gtccgatctt cctggcattg ggctttggtg gcctgcatcc
     6601 tgcttgggat catttccaaa ttgccctggg ctgtatcgac cattggctat ttggcggttg
     6661 tttttactgc tcaggtcttg caaaggcgtg tgtggcaggc tccattgctg gcgatgttta
     6721 gtgttacact cattggtacc attttcatgc atctgctgtc ttataccgtg ttgagcctgt
     6781 ctggaacgcc attttcattc tcggctgtgg caggttcgat caccttgcca agtttattgt
     6841 tgaatatttt cttttcgatc ccgatctatg ccttgatgcg cgatttggcg cgttgggtat
     6901 atccatcaga ggtttatgaa tgagtagcaa ttctgtacca cgctccgttc gttttgaagc
     6961 ctggcggctt gtaaccgttt atgttgtggt tgtgttggtg tttgtggctt ttcttgttcg
     7021 cttgctcaga cttcaagtca ttgaaggagc gaactggaag accagcgccg ttgataacta
     7081 tacctttgaa gtcagtgttc ccgccccgcg tggaattatc tatgatcgga acggctatat
     7141 ccttgcacgg aatgttgcct cctacaacat cattatcaca ccagcaaacc ttcccgatga
     7201 cgattcggat attcaggcga tttaccgcga cctttctgac ctgctggatg tgcctgtagg
     7261 cgggcgcgcc gatgacgagg ccctgctcga agaagccaag ttattcggcc cctgtgtgcc
     7321 ggggccgggc attactcaac tggtggcgtt gcaagattcg cttgctccgt acagcccggt
     7381 caagatcaag tgtaatgtca ctgtggatat tgcccgcatt gtcgaagaga aatcggtgga
     7441 ttggcccggt gtgacggttg aaattgagcc gatccgtgat tatccgacag gctcgctcac
     7501 ctcggcgctg gttggattct tggggccgat ccctgcttcg ctggaggagg agtttcgcgc
     7561 ccagggtttt atccccaacc gtgacaagat cggttatgca ggtgttgaag attccttgca
     7621 ggaaatcctt gcaggcagga atggtctccg cgtagtgcaa gtggatgtag ccgggcagga
     7681 acttcgcaac cttgagccgc ccatcccagc tgtgccggga aacaatgtaa ccctcacaat
     7741 tgatacccgt ttgcaggcag ttgcagaagc ctccctgatt caggaaatca atttttggaa
     7801 cgattacttt gacaccattc gtatttccag cggtgtcgtg atcgcgatga acccgaagac
     7861 cggtgaaatc ctggcgatgg tttcctaccc aacttatgag aataatcgat ttgcccgatt
     7921 tatccctgct tattattatg aacaattaag tcaagacccg cgccatccgt tgttaaataa
     7981 tgccatttct gcagagttcc cgcctgggtc ggttttcaag ttgtccactg caacaggctc
     8041 tttcaacgaa ggcgtgattg gacccgatga tatagtggat gcgcctggta aattggaatt
     8101 gtgcgaaaaa ttcaatccca atgatccttg tactggtttg aatacccgtc cattcgtgga
     8161 ccatattttc gagaaaaagc ccgaagggtt tggcccgatc agtttttatc agtgcattgc
     8221 ctactcaagt aatgtatgct tttataaact gggcggcggc tatcaagatg aaattgaaca
     8281 gggtttgggc atcgaacgtt tgcagcaata tgcccgcgct cttggttatg accagccatc
     8341 cggtatcgaa ctaaaaggtg aacaggatgg actgatccca gacccgaagt ggaaacgcat
     8401 caacaccggc gaaaactggt ctacgggtga tacctatatt gccagtgtgg ggcagggata
     8461 tgtccttgcc accccgcttc aaatcctcat gtctggagcg accattgcca ataacggaaa
     8521 gttgatgcaa cccaccatta tccgcgaagt aaccgatggt gatggcaagc ctgtggacat
     8581 ctggttcaac cccgatcctg aggtcttcac cgccacagat tttgaagtgc ctggcagtta
     8641 tcagatttct cctttcaccc ccaacctcaa atgggacgtg accgtgaccc cgctgattca
     8701 aagttattcc tgtgatgcgg gatactgtga tctgaatgaa aacgacctca agacgattcg
     8761 ccccgaggca gttgcagcgg tacgtgcagg tacaaggctt gccgtaacag acccattctt
     8821 cggcaccctt tatgacgtat tcaacgattt cccgatcgct gttgcaggca aaacgggaac
     8881 cgctgaatac tgcgatgatg tggctcgcaa ggccgatcaa tgtgatttcg gtaaatggcc
     8941 cacccattca tggactctgg cttatgcacc ctatgacgat cctgagatca ttgttgtggc
     9001 ttttgcctat cacggtggtg aaggtgccag tgtcgcggcc cccattgtat cgcgtgtcat
     9061 caaggcttat ttcgagttca aggcccttga tattgcgcag ggaaaccctg ctttgggcca
     9121 ataatgacca ttgtcttcaa attgcatttt acgaacaacc aattcaattt gcctgacacg
     9181 tcaggtataa tttgagccat tatttggtca aatgtgatat ggagcaaatt acttctctgg
     9241 ttcagatcaa agggattcgt gatggacttc tcgcgacgtt ttcagaagcg ccgtgggagg
     9301 atcaatgcct tgccttgttg acgcagattg acgagcgccc tgcttttttt caaggtgccc
     9361 gccttgcgat ggacgttggt acacaggtcc tgaaggtcaa cgaccttgta gatttgcgcg
     9421 accgcctttc ggaacgaaat attgcacttt gggcggtgat cagcgagtcg ccaaccactg
     9481 aacaaacttc acaattgctt gggttggcca cccgcatctc gaagccgcga ccggaagaac
     9541 aacgtcaggt cgcagactcg atctcagatg acagggcgct ttttatcaat aaaactctcc
     9601 gttcaggcac acgtgttgaa tttccgggga acattgtcat tgttggtgat gtgaatcccg
     9661 gtgcggaaat tgttgcagaa ggtaatgtgg tggtatgggg cagggtgcgc ggcatgatcc
     9721 atgccggggc aaagggagat cgctccgcct atatttgcgc acttgatctt tccgccaacc
     9781 aactccgcat tgcagatgaa gtctcggcta tgttgaagcc tcaaaaagac cccaagcccg
     9841 agatcgccac cattaatggt gaaggacgat tgcaggctga actctggcac gtcggttaaa
     9901 gaatctactt taggaaatga tatgactgct caagtgatca ctgttacatc tggaaaaggc
     9961 ggcgtgggta aaaccacagc cgttgcaaat ctcgccactg ctcttgcaac agatggcagg
    10021 aaggtcgtct gtattgacgg cgatatcggc ctgcgcaacc ttgatgtcat catgggcctt
    10081 gaaaaccgta ttgtgtacga tattgttgat gtcatcgaag gccgctgcaa attgaagcag
    10141 gccatgatac gcgataagca tttcccggat atgtacctga tccccgccgc gcagactcgc
    10201 gacaagaatg cagtctcacc ttcagacatg gtgcgcatct gcaatgacct tcgtcctgat
    10261 actgatttca ttatcattga ttcacccgca ggcattgagc gtggattccg caatgccatt
    10321 gcggccgctg accgtgtcct tgtggtgacc aaccccgagg tcagcgcagt acgagatgca
    10381 gaccgtgtcg tgggtatcct tgaagcggaa gaaaaaggca gcccggcgct catcctcaac
    10441 cgcttgaatc caaccctggt gcgtaataac gacatgcttt cccctgaaga tgtgctcgac
    10501 ctgcttggaa ttcaactgat cggcatcgta cctgaggatg aatctgtgat tatcggttcg
    10561 aatcgtggcg ccccggttgt gaccgataca aaaagccgtg cggggcaggc cttccgtaac
    10621 attgccaaac gaattcaagg acagaacgta cccttcatgg accttgagca gcaaggcggc
    10681 ttgtggggtg ccatccaaaa actgaccggg aggaagtaag atgaacttct tcacacgaaa
    10741 gcgcagcgcc gaaagcgcaa aggaacgttt gcaattggtc ttgatccacg accgcactga
    10801 cctgacccca ggccagttgg aagaattgaa agacgacctg ctcaaagcga tttcccgcta
    10861 tattgacatt gaccctgaag ccgtgcagat cggattggaa cgtgacggcc gctcacagcg
    10921 gctggtggct gatattcccc tgcggagcgc atcgcgccat cgtgcaggtt gatctgtaaa
    10981 accgtacaag cacggatcag ccttgcggaa acgtctggtt gtatccgtgc ttttttgtta
    11041 actaaggctc tctatgaacc gtgaattttc ctggcgaaat ttcgacttta ttttactggg
    11101 cgcaattgtg ctggcctcgt cttttggcac ggcaatgatt cgctccgctg tggcagggaa
    11161 tgaggtcttg cagccgttga tcatccgtca ggtatatttt gcaatccttg gcgtggtctt
    11221 gatttttctg gtcggttcga ttgattatcg ttattggctg gccttgtatc gcccgatcta
    11281 ctttgggatg atcgcttttc tggtcacatt gacaggcttt ggccagtcgg cttttggtgc
    11341 ccaacgctgg tttcaagtgg gtgtgttgtt tcttcagcca acggagtttg ccaaaattgt
    11401 cgccatcatt gttctggggc gttattttga ggtggctcaa caccaacccc gtgacctgaa
    11461 atgggccttt ggtgccattg cctgggctgc cggtatcatc attatgattt tgctgcagcc
    11521 aaacctgagt aatgtgcttt tgctttctgt cattcttgct gtaatgcttt ggatgaacgg
    11581 tgtgcaggtg aagcatgtgt tttctgctgc tgccatcgga gccgcagcgg ctctttcgct
    11641 gatagtcctc tctgtacttg gcatatccat ccccgggtta cagccttatc agcaggaacg
    11701 tattgtgaac tttgtggtgc cagacccaaa cgacacctac ggtaaccgct ataatgtaca
    11761 gcaggctttg atcgctgtcg gagcaggcgg cttgtttggt gaaggatacg gacatggaac
    11821 ccagacccaa ttgcgttttc tgaaagtccg ccacaccgat tttatcttcg ctgccagttc
    11881 tgaagagttt ggcatggtgg gcggcgcgct catcattatc acgctgggaa ttatcgtttg
    11941 gcgctgcatc cgtgctgcgc agaaatcgcg ggatgtggca ggagctacga tcgcgatcgg
    12001 tgtcgccacc ctgatctttt ttcaagccgc tgtgaatatt ggtatgaacc tcaatctgtt
    12061 gccagtttcc ggtcttccgc ttcctttcat cagttatggc ggcagcggcc tcaccgctct
    12121 catgcttgga atcggcctcg ttgaaagtgt cgtcatgcgc cacaaacaaa tggaattcta
    12181 accagcacct tcaaccttaa actttcaacc taaaacctgt aacctgcaac ctaaatcctg
    12241 caaccctccc aaaggagtcc acacttgacc acaaaacatg cacgtactcg ctttgccccc
    12301 tcgccaacag gacacttaca tcttggcggc gcacgtaccg ctctgtatgc ttatctgctc
    12361 gcaaaaaaaa cgggcgggca gtttctttta cgcatcgaag acactgacat aaaacgtacc
    12421 gttcccggcg ctgaacagga aattatggat ggtttgcgct ggctgggtct taattacgat
    12481 gaaggtcccg acattggcgg aaaatatggt ccatatcgtc aaactgagcg ccgcgagatt
    12541 tatcaggcgc atgcaaaaac acttgttgaa aacggccatg cttatccctg cttttgtacg
    12601 ccagagcacc ttgacaaggt gcgccaggaa cagatgaagc gcaaggaaaa tccgcgctac
    12661 gatgggacct gccgtgtgct ttccgctgac gaagccgcaa aacgcattgc caatggtgag
    12721 ccatatacca ttcgcttcaa ggtgccgtat gaaggttcca ctgtggcgca tgaccatttg
    12781 cgcggcgaca tcaccatcga aaacaaacaa ctcaacgatc aggtcattct caaaaccgat
    12841 ggaatgccga cctatcatct tgccgcaatg gtggatgacc acgagatgca gatcacacac
    12901 gtcatacgcg gctctgagtg gctgggtacc tttcctttgc atgtcaacat tgtccgcgcg
    12961 tttggctggg aagagcctgt ttgggcgcat ctatccgtat tcctcaagcc gagcggcaag
    13021 ggtaagatgt cgaaacgtga tgcgcccgat gccatgaaag atggctattc ggtctttatc
    13081 aaagacatgc aagacctcgg ctttacgcct gaaggtgtat tgaattggtg cgcattgatg
    13141 gggtggggcg ttgctgaaga tgatgtgatg accgttgatc aaatggtgga tcgctttacc
    13201 atcgattcgc tgactccttc tcctgctgcg gtcaacttcc aaaggctgga tcatttcaat
    13261 gcgacacata tccgcctctt tacgaccgaa gacctggctg cccgcatcaa gccttacttt
    13321 acccgtgagg gactcaaagt ggatgacggc attctgctaa aaattgtgcc gcttatccgc
    13381 gagcgcctca ccacgttgga tgattgtcta tcgtttggaa gtttcttctt caaggaagat
    13441 gtatcgcctg cacccgcaga cctgatcgcc aaaggattgg acgcgaagca atcggcgcag
    13501 atcgcccaaa gggcgtacga gattctcgtg gcgcaaccag atatcagtca cgaaagatgc
    13561 gagccgcctt tgcgtgcata cgtcgaagag agtggactca atgcaaatca agtttttggg
    13621 attttgcgcg tagccacgac agggcaaaag gtcagccctc cgttgtttga aagcatggaa
    13681 atcatcggca gggagaaatg ccttgcacga attaaatctg cgattgaatt gctggaaaaa
    13741 atgtgatcga aataaaaagc tcccgccgtt tctgtatggc gggagctttt ttgttgggct
    13801 atttatcagg acgattgacg aaataaatgc tggccattac cagtaaaatc gtcaggatga
    13861 catggaagac cgtgtaaatg atccatggag atggcagggg gagtgatgtt gtgccaagca
    13921 taccgttggt ggtgaagaaa atactttgat cgctgacaag aatggcttca cttgcaacgg
    13981 ctgcaaagaa tgaatttgta gaaaacagat accagactga aatccccaga acctcttccg
    14041 agattcccga taggaaggtg tttgtggagc ttgccacacc tgcaaagacg aggataaaaa
    14101 agaaaagcag aaccatcaca ataaaggata gcatgatgga tgcgtaggcc gccacggtgg
    14161 aaaccagggt gcgctttgta aaacttgaaa agaaaagtcc cagggcgctg aagaaaaata
    14221 tggaaaccac caacatcaca atggaaatga ccatttcggc catgcccaca ccgcccaaaa
    14281 agaacgacat gctttggatc gggatggcgg taaaaataag cagaagcaga aatacaactg
    14341 ccgaactgag tttcccgaaa accagagcac ggcctgaaag cagcgatgtg cggagcaggt
    14401 cgaaggtttg gcgctcgcgc tcggaggaga tcgctcctga ggtcagcgct ggcccaatga
    14461 aacttaccat gagcaattca agcaagacca ctgtgctgaa tatgatcttg ccgacgattt
    14521 gcagataacc agggtcgttt ctggacagac ttccatccac gctgagaaag agatagacca
    14581 ggccgataaa aaaaccaatc aaggcaaggt aggcgctgag caaaatgaat ccctgcctgc
    14641 cgcgcattct gcctttaagt tccttggtta tgacggggtt atcaagcaga agttcaagcc
    14701 attttctgcg ggtgtttttt tcaggcacaa cagatgttgc agatgattcc atgattttcc
    14761 tttaagacac aattccacgt gtggtgcgca taaagacttc ttcaagattt ttggtttctt
    14821 cgctgaaatg caccacggga tagccttgtg cgagaagccc agccagcaat tggtggatgg
    14881 cttcgtcgtc tccgataaaa tccacttcga gggtggggcg ttcggtgttt tccaatacgc
    14941 gtgcctcgtt gacacccgct cttgattgca gattgatctg cgcttcctgg gcgcgatcga
    15001 gcaggctgat gcgcagttga cggtgaggca tgacgcgttc actcaattgc tgaagtgtgc
    15061 cgatggcagc cagttttcca tcttcgatga tgccgacacg tgtgcaaagt tcgctgacat
    15121 ctgcaaggat atggctggag aagatgatgg ttttgccaag cctcgcgatt tcaagcagta
    15181 gttcgcggat ctccactcgt gcgcgcggat caaggcccga ggcaggttca tccagcacga
    15241 gaatgtttgg gtcgtggatc aggactcggg cgagtccgag gcgttgtttt aatcctgtgg
    15301 agagggtatc caccatgtcg tcgcggcggt gaccaaggtc cacgagttcg agcaggtcat
    15361 tgatcaatcg caagcgttga ttcggaggta ctttgtagca ggcgccaaag aaatcgaggt
    15421 attcgtgtac ggtcatatcg ccatatacgc cgaaagaatc gggcatgaag ccgatcatgc
    15481 ggcgcacatc gtttggattc tttctgatgg aatgcccgcc gacgaagatt tcgcctttat
    15541 cgggtctcaa caatgtgacc aacatgcgca tggtggtggt tttgcctgca ccatttggcc
    15601 cgaccagccc gaagatttct ccgctttcca cgttgaaact gatgttgtta acggcaaagc
    15661 gtttgccaaa atgtttgcac aggtctttga tttcgatgat ggggctcatg gctgcaccgt
    15721 catggtgaaa ttgatcgccg tgatctcaac ataatcattg gggtcacctt tgaggtttag
    15781 cctgacttca ccatccatgc cgatgtattg ctgcggattg gggatgtcga tggtgtaaaa
    15841 ggtcatggga atgaggtccc aggtttgggt ttcaaaattc cacaaagaga caatgacctt
    15901 gtctggcgtt gtggtggatt cgatgtttaa ttcaaaagta ttcaccttgc tgaagcgaat
    15961 ggtctggttg ggttgatagc gcagggtgta gccattgttc gacatgttgt agtatggggt
    16021 ggtgatggtg tctccaagag acgaatccca tgagaaaatg ctggaagtca acatgagcga
    16081 gccagcctcg gttttgatgc tcggcgtaag ccgttcgaaa aagagcatgg tgtcgatctc
    16141 ttcctggggt tgtgattgaa gacctgtggg agtggcgatt tcatcattca cccagcccat
    16201 caaatatatg ccagaattta cgtggatgct gccgttgctg gtgttggtgt tggcctggaa
    16261 aaaggcggcc tgtcttttct tttcagggtt gacgtctgaa taatagtcat acgtgtctat
    16321 attaagcgtg gtcatgatcg tgtattgact tgtcactgag gtgttcgagt tgtttacaag
    16381 gatttgactc acctttttag tttgccctgg agcgatatcg cccagtggtg accattgact
    16441 ggctgtgacc agaacggcat ccttgatggt gtagtcgcct gtgtttgtaa tcgtgccaga
    16501 gagttcaggg acgtgactgc caagcgtaat ggtcaggtcg ctttgaacgg taagcgcagg
    16561 caggtaacct gttgcaccga cgacctgcat tccgccgatt tccacgcgta tgtcaggcat
    16621 gcttgtgcca tcctgggttt tgatggatgt ccaatcatca ttgccctgca ggttcccgct
    16681 tgtgatgggt gtgggcctga gcatgaattg ttcctgtgtt tccactgtgt aggtggtgcg
    16741 gctcggcgaa tataccccga tcacagcgtt gacctgtgac tggctcacgc cttcccagcc
    16801 ttgagccatc atcagtcgat tcagaacagg gcgtgtgccg cgataggcat acccggagaa
    16861 atacgccagc atggtgaaca gaatcaccaa gatcggaatg gaaacccagg ccaactcagg
    16921 gcgtttgatg cggcgcaata caaagtagtt caatggcccg actacaaata cgtacaaacc
    16981 aagccagcag cagatataaa caaaggaagg cagggccaat tctggcatgg aggaaagcgc
    17041 atcattgatc tgatacgcat cccaataccc atttgcccaa acgggtttgg gcgatctgaa
    17101 cccaagcaaa tgctcataaa tggccagcat gccaacccaa ttgttgagcg gctccaggcc
    17161 agggtctgct gcaaaaaagt acactttacc aaaaccgacc tgtttttcaa ccaacacggg
    17221 gattccgctt tgatcaacga gggtggttgc cccttccagt aattgtcctg ttgccagaac
    17281 tgctgactgt tcttcagtta ataatgggaa ttttgaatat ttggatattg ccgaaagatt
    17341 tgtaacttct ttggttgagg tcaattttat tggcagaata ttttccaacc ctgctgcagt
    17401 tggctgccaa tttgtgccgc cagtgacgaa taacctgccg cctctggcaa gccacatttc
    17461 catggcctgt ttttgctctg cgctaaattt gccagtgtcc acattggaaa tgaccaatgc
    17521 gtccatgatc tcccagcctt gggcctgatc gggcaggtct tcgaccttta attgtgctga
    17581 ttgtgcgttg acgccagaca acgaaatggt gttcaagcct gcaaagacag aagggtcatc
    17641 tgtcacaagg ccaaacaaca gtgtttggtc gttgatgcag gcaatgttga gggcggtttt
    17701 ggcaagggtt ttattgccat caagcacgct gacgttaaag ttccgtgcgg tcccatccaa
    17761 aaaaacgtaa atgaaaaatt ccttgcgtga agtggctggc agtgaaatat ccatcccgta
    17821 caccgcattt ccgttcatgt cgtttttgta ggaggcttgc acgcgtgcgt caacatcctt
    17881 gccgttgttt tccaccatca ctttcacggg cagccaggtg ccgttcttgc agaaattatc
    17941 caaccccgat tgcgccttga tggtgatctc tccctgcgtt tgggcggcgg ctgttgtact
    18001 tgtccacgag gtgaaaagta ttgccgcgaa aatcatcgct aggaaggcag tttgtttgta
    18061 aatagctgag cgatatggaa gtttcaagcg ttttcctcca acgggctgat gatgggtgaa
    18121 atcatatcat atttggttta ttgctttatt gctctgcatg tatacttggg tcatgcctgc
    18181 cattgaaaaa ttccagacaa actccctgcg ttcgcagaaa tatgatgctg aggtgacgcg
    18241 tattttggca gcaatgttcc agtcagttga gcctggagcg gccgtgaaaa ggcacttgcg
    18301 cgaaaatccc ctgcccgcag caaaccgcac ttttgttttt ggattgggaa aagccgcctg
    18361 tgccatggct tcagcgctga cggatgaaat ccacccgata gacagcctgc tcatcaccaa
    18421 acacgcttcg ccgctggacc tgaaacctgc cacggtgatc gaggggaatc atcctgtccc
    18481 aggcagtttg agtctcgccg ctggagccgc cgcgttgaag ttcctctcgc aattgacagc
    18541 agatgacctg ctcatttgcc tgatctcagg cggcggatct gccctgatgg ctgccccgcg
    18601 tattccactg gtggatttgc aaactctgac ctcggcgttg ctggcttgtg gcgcccgcat
    18661 tgacgagatc aacattctcc gccgacatct ggatcagctc aagggtggcg gcattgtacg
    18721 gcacgccaac ggtgcgcggg tggtcagctt tctcctttca gatgtggttg gtgacccttt
    18781 ggaagcgatc gcctctggcc cgaccgcacc cgacccaacc acctgcgcag acgctcttgc
    18841 tgtcattgaa aaatacgacc tgaaagacaa aattccatct tccatcctgc gtgctctgac
    18901 ggaaaccctg aagcccgccg accctctctt tgcgaacgtg caaaatacca tcattgccag
    18961 caatgcaatt gccttgcgga ctgcgcaagt atgcgcccag caggaagggt ttcaaacccg
    19021 tgtgtggcgg tctgatatgc aaggtgaagc ggctgttttg gggcgtgaaa tggttttgga
    19081 gttgagcgag atgctcaaaa atacgccgcg tccattttgc ctgttggcgg gtggcgaagc
    19141 gactgtcact ttgaattcgg tgaaaaatgg aaaagggggc aggaatcagg aatttgcctt
    19201 gggagcagtg gatgagttgg cgggcatggc tgatgttttg ttgatctcgc ttgccacaga
    19261 tggagaggat ggccctacgg atgccgcagg tgccatggtg acaggggctt ctgctcaaag
    19321 ggctgagtcg cttggggtga gcgtggcaga ccacttgtca gggcatgatg cgtatccctt
    19381 tttcgagatg ctgggtgatc tggtcagaac tggcccaagc ggtacgaatg tcaatgacat
    19441 cgtcctctgc tttgcgtttt gattcaaact atggcagggg aatgtctttt acgatttcca
    19501 tcatttcacg catgcgccct gtggctgctt ctatgccttc ggggaactca tcgaatcgtg
    19561 tggtttggaa tttttccaaa gctgtttggc tgtttgggtc ttcgtgcatg gtggtcagga
    19621 tctgaacaat ggcttgcagt aactcgggtt cgaggcctgc gcgtgccagt cccacttgtc
    19681 gcggcgtgga ttcggtgcgt gccaattcca ccagctgcgc agtggcctcg gggggaaatg
    19741 cgtggtcaaa attgtaatca tcggtgacgc ctgcgggggt atgtccgctc aagacccatt
    19801 gcaaaatatt ttcatcatcg taggcaaatg caaagccgac ttcgttgctg tcaacagttg
    19861 cggtgctttc agatttgccg atgagtttta gcccattttc agtaagatac acggctggca
    19921 gcataaaccc cgaagtggag tatggtgtat ccattgcgat cattttcccc ttgagccctt
    19981 caatactggt gatgccgctg gctttgcttg caaatatgac gctgttgtac gtttccaccc
    20041 cgaaacgcca gcggcgcagg atgatttgcg cgcccgaggc atcgctgatc aatgtggcgg
    20101 ggtaggtgct gtcgaaatac acatccactt cgccgcgttt gagcagctcg atcatttctt
    20161 cggtggaggc cactaccttt acatgaccgc ctgtaatgcc gtatgcggca agtttggcgg
    20221 ccacgtaatc tgcgatcggc tgagtccctt caatgacttc ggcggggtcg tctgaaatat
    20281 cgccgaagac gatgaaatgc tgttcctgcg aaggagtgtt ggttgcaggt gggtttgttg
    20341 tgggtgatgg attggggctg gtgcaggcaa caagcaggag agcaagggcg aacaggcagc
    20401 ggaatgcgct ttttgtcatg aacctcatcc tggaactgga tttggttcga ttatagttgt
    20461 gcgagtgcga aggctccgaa tgttgtaatg ataatgccag agaggcgatt gatccacatc
    20521 atgtggacgg gagtaagacg atcgcgcatc agtccaaccc cgaaactgag tgccagccac
    20581 caactggccg accccgcgaa aacccctgcc accaaaacca tgggtgagct tgttgctcca
    20641 atgttcattg tccctgcaaa aatggccgca aaagacagaa tggtcatggg gttggtgatg
    20701 gtcaggaaaa aagtggaaag atacgcgcca aaatatccgc tctgtttgac ctgtgcggca
    20761 tggtcggcgg gtttttcaag gaaggttttg attccaagat atattagaaa gcagccgccg
    20821 atcacccgaa accagaactt gttttcaacc aatacgtcgg tgatcatggt caggccaaaa
    20881 gccgcaaccg cgccataaac catgtcggca ctggcggcgc ccatgccaga aagaaagccc
    20941 ttgaaccttc cctctgtcaa cgtgcggcgg atgcatagca cgccgatcgg cccgacgggt
    21001 gcggcgatgg agatgccaat tagaaatcct cggaaaagga ggagcagggt ttccgtcata
    21061 aattctccaa tgtttgaatt agaatcaaaa aaggacatcg aaactcgatg tccttttttg
    21121 taaaagttag atcgcagttt cttctttggg cttaaaccag gttgtccatt cgcggttttc
    21181 ccaaaccact aaaatggggg ccgcattgaa gatggaagag taggttccgc tgaaaatacc
    21241 gatgagcaga atgaccacga agtgacgaat gctgtcgccg ccgaaaagaa ccagcgcaaa
    21301 cagggtgaac atcacggtca attgtgtggt gatggaacga tccagagtct gcacgatgga
    21361 atggttgacc atcttttcgt attcgatctt gcgaagcagc agggagtttt cgcggacacg
    21421 gtcaaacacc acgatggtgt catgcaccga gaaaccgatg acggtgagca gggcggtcag
    21481 gaagagggca tccgcctccc agccgaggta atagccaagg atggcttcca cacccagtac
    21541 caccaacacg tcatgaagca tggcgaggat ggcggcggtg ccgtagcggt aggcatgttc
    21601 cacactgcgg aaggcccacc aaatgtagat caggatcgcg atggcggcga acaaaatagc
    21661 cacaccagcc gcctgagtta cttcggcacc cactgaagcg ctgacggagg tgaagttttc
    21721 caccgtcact ttggagccga agcgggattc catttcagcg acgatcaaac ctttggtttc
    21781 atcggtcatc gccttggaac ggatggagat tgaactctca cccaaagact gaaccacagg
    21841 gtcggcgatg ggttgttctt ctgtggagaa atcgctgtaa agtgcagaga tttcctccgc
    21901 agtggggcgt tcgccttcga attgcacttc aagcagcgag ccgccgcgga agtcaatgcc
    21961 aagctgcagc ggaccagcct tggtttgctg ccagttgata cccatgaaga caatgccagg
    22021 gatgatgacc agcagggaga tgaggaagta aatgtaacga tttttgatga tattcatgac
    22081 gcgctcctag agaccgaacc agcgcggatg ttcggtggtt ttgacactgt caagcacgct
    22141 gtgaaggaag gtgcgtgtga cgatgatggc cgtgaaaaga ctgaccacaa cacccagtga
    22201 aagtgtgacc gagaaaccct tcacaatgct ggcgccaaag gtattgccga agatgaaaag
    22261 gatcgcacag gtgatcaatg tggaggtgtt tgagtcacgg atggaaggcc aggcgcggct
    22321 ccaggcgagg tcaatggcct gacgcaggtt gcggccggaa cggagttctt ccttcaagcg
    22381 ttcgaagatc aacacgtttg cgtccactgc catacccaga ctcaaaataa aaccagcgat
    22441 accaggcagg gtcaatgtga cgggaattaa cttgaagagc atcagggaga acagcgtgta
    22501 gcagaccagg gccagatcgg cgatgacacc cggtaagcgg tagttgaagg ccatgaacag
    22561 gatgaccacg atcataccga tgataccggc ctgaacgctc ctgcgaatgc tttcttcacc
    22621 gagggtggcg ccgatggtgc ggctctgaac caccttgatc ggaatgggca gggcgccaga
    22681 gcgcagctgc acagccaatg tttgagctga ttcctgagta aagccgccgg agatctgtcc
    22741 ctgaccatcg gtgatggcct gattgattct cggcgcagag atgacacgtt tatccagtac
    22801 gatggcgaga tactgcccga cgtgggagct ggtgtattca gaaaatacat cggctgcgtc
    22861 agaatccagg gtgaaggcaa ttgcgatctg gcccgagctt gggtcacgtt gaacgacggc
    22921 gctatccaaa ccggcaccgg tcatgaccgt gtggtagatc gtttcgccgt tttcattggt
    22981 cgtttggccg ggggtttcaa ggccgaaatc ggtcagaatg gtggtcccat cgggaacagg
    23041 ggcgctgccc atgtccacaa attccaacag agcggtctgt ttgagggcgg ccaccatttc
    23101 ttccggattg gtaaggcccg ggaattcagc cacaatacgg cgttcgccag cagtcaccat
    23161 ggtaatttcg ctgacgccga gggcgttcgc gcggttttgc aggatgttgg tggcgttttg
    23221 cagttcttct gcagagacgg tggtttctgc gggtacatcg gcttcaagga gtgcctgaag
    23281 gcctccacgc aggtcaaggc ccagtttaac atccacgttg cgttcgacga gcggcgtatt
    23341 gttaaatggg ttgtagacta cgatgtcctt gctgaggtct atccatagcg agaaggctac
    23401 gagtagaagg gttatgatta accagctatt tctattgcgc atgttttaac tccatccacg
    23461 aaaatgccag ctttgggctg gcgcggacgc cattatactc cgaatgaata atttatgggt
    23521 aaatccagct tttaaagaga tctgtcaggt cacacgtgca gttttgttcg gcgagttgtt
    23581 tcatcttttc gggggtggca atgtcccagg cgttttcatt tacatatgcc ttcatgaatt
    23641 ggtcaaaatt ttcagagccc atttcatcgc gcagggcctc gaagaagagt ccgccgcggc
    23701 cgtaaacgat gctgccatac tcgatatcct tgtaatcgcg tacgggcatg ccgacaggga
    23761 tcgctgcgtt gttggtccgc gcccatcggc cttccaggct gaagcgaaag ccttcgtaac
    23821 ccgattgccc atattgatct gaaaaatatt gcagggtggc aaactgcgtg aggctttcat
    23881 cgagccacgg gtcatccagt tgatcattgc cgaccaggtt gtagaaccac tgatgtccca
    23941 cttcgtgtgc aacggtccct tcaaggtatt cgtgatacgg tgagacgatt ctatcggcaa
    24001 tggcgatcat gccggggtat tcaattccga gtgcgagtgt gggtgtagag acaaaatcca
    24061 attcggtgta ggggtaggcg gcgtagcgat tttcgtacac ttcgatggat ttggcggcga
    24121 tttcaagtgt gcgttcggcg ccagatcttt gatcggcggg cgcatagaat cgaagtgtga
    24181 ctccgtttga ttctttcgtg acgacttcat agtttggact cgccgccaga taataatcac
    24241 gcacaggccc cgcaacatac tggaccgttt gcctgtttcc gttgcgctcc gcttgacgaa
    24301 gaatctcccg cccaacactg accagaacca gatccttcgg cgcatccaca gtgacattga
    24361 aaaatgacat gtcggcgtaa gtcacatcgc ctgattgtgg ggggatctcg gcgttccagc
    24421 cttcgtcgtc gtagactgcg atcatggggt aggcgtgtgc cagtgtcagc acgtcgttga
    24481 aataggcttg cacaccgtaa ttcaattcaa ccgattgcgg cacagtgacg gtaaacccca
    24541 tgctcagggt gatggactct ttcggttgga gcggttgttt caacggcacg gtgagcaggc
    24601 tgtcatcgag ggtgtaattg gggacgacga tcgttccgcc tgcatccact tcgtccacgt
    24661 gcatttcgcc gccgaggatg ttgggaaaca ggcggaattt gacttcattt aatgcgctat
    24721 cttctgcgtt ggtgtagatg acggtttcat ggcctttcac atggtaaagg tcatcttcga
    24781 tggtgaagtg gatttcgtag agactggctg tggggagttc atttaatacg ctttgatagg
    24841 caggcgtgag accatcttta taaatggaac ggtcggcggg gtctgcggtt gggtttggat
    24901 tctgctgcgg ggttgattct gctgcttgtc tggctggcag gcaggcgggt aggagaagaa
    24961 gcgcaagcat caggagcagg aaaatctttt tcattgggta aattccttta agaaggtcaa
    25021 agcccgggcg aggacatcgt caggtttcaa caggtcggta tcaatgatga tgtgggcatg
    25081 ggtgcgggca tgcgagagcc tgcgctgctg ctcttcgtaa tcggcgagcg accagtttag
    25141 ttttctgcgt tgggtggagt tttcgaacga ggagtgtaga aagatgagga tatctggatt
    25201 ggtgatgacc tgccacatgt gtttgacgta ggaatgttcc tgtgcgatgt ggcggcagcg
    25261 atagccatgt gtttccagcc cagccataag cgtggatttt cccgacccgc atgggccgac
    25321 gatgccgatc agcggggtac tgtcttcatc cttcattgtc atgcaacagg tgactgagct
    25381 gctcaggcac accgaggact gcgatcgtat cccccgcatg cagtggactt ttatcggtgg
    25441 ggtgcatttc agattggtgg tcgtggcgga ggaggacgat gttgaggcgg tagttatctt
    25501 ccacgtagcc gatggttttg ttggcgaagg gtgctttttc tgaaacggtg agccgcgcca
    25561 ggctgagcag ttgaccttcc accgaaatgg ggttcgtaat atcgacacct gaggcggcgg
    25621 cggcaaaaac gggtgcggcc atttcggtgg cgctcagggc cacgaatccg aattggtctt
    25681 gcagggagtg ggcgaaatcc tcatcgaaga tgcggacaac gactttgatg tttttattca
    25741 gggtgcgggc tttgagggcg atctgcaaat tcatggcgtc gttttggctg gccatgatga
    25801 tggtgcgcgc cgattttaca ttggcggctt cgagcgagga tgggcgtgtg gcgtcttcat
    25861 gaatgacggg gatgcccaac ccacgcacga cgctgagtgt gtcggcgtcg gcgttgaatt
    25921 cgatggcggc gatctgttcg cccatttcat gcagtttgag cgcgacacga tagccgaggt
    25981 gtcccagccc gacgaggatg atgtgatttt tcaaagtgga tgcgacggcc atttcccatt
    26041 ccttgttgcg cgagcggcga ttgaagagca ggctgccgaa atcggccaat ccctgcgcga
    26101 gcatgatgat gccgatgatc ggcattaaaa agtgaaataa ttgcagaacg atatgggttg
    26161 gaaaacttcg attgggcggt tgtaaaaacg tgatggtgag gacggtgtaa atggattcgg
    26221 ccaggctgcc gacaggttca ttgagaagcc tggacaaaaa atcgtaaaga atgcctccgc
    26281 ccaggatcgc cacggaaaac cacaaaatgg cggagcggaa ttcgcccagc aggatcatcg
    26341 tatcgcggat ggatgcctga aaatgacgcc agcgatgttt gcgtgaacct gaatgatgag
    26401 agtgggccgt gctagccatt gaatgggagc ctgacgttca tgcgccgtac gtggtcgcct
    26461 gcgcgtggag agactttcaa caccagcacg ctgatatcgt cggcggggcg gccatcatcg
    26521 agttgtacgg catgggcgag gagcgaatcg gcaagttgct gcggggaggg gtcttgatct
    26581 tcgatgaggg cgcgcagggt ttcagccacg tccatgggtt ggccgcggcg tttccccgcg
    26641 tggacaatgc catcggtgaa aatgacgatg gcgagttcgg gttccacggc cacttcggtg
    26701 atgacgggcc gtacgttgcg cgaggtgccg agcggttcgc tttcttcggt gtgggcgtcc
    26761 acttgttcac cccgacagat gaagactggg gaaggattgt tgcgggtgag gacgatggtg
    26821 ttgctgtgca ggtcaacgga ggcgatgttg agggtggaaa tgacctttcc ctgtttctca
    26881 gcaaacagcg cgtccgatgc ggcgcgggcg gcggctccat cgcggacgcc ttcggcgatc
    26941 agcccgatga ccttgcgcac gaccatcatc gaaacggctt tcgcgccccg tccgctggtt
    27001 tgaccgtctg ccagcacaac ggagagtccg ccggtggggc gttccaccac ttcgagcgta
    27061 tctccgcttt cagagacggc gtatttgttg atcttggcga ctgcgatttg aatttccacg
    27121 aggatgattt tactatctgt tccctgtttc aaacaggtga tgggattttt ggaatttgga
    27181 tgaaccagag ataaac
//
LOCUS       DAOQLC010000057        27122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_586_length_27122_cov_22.122917, whole genome shotgun sequence.
ACCESSION   DAOQLC010000057 DAOQLC010000000
VERSION     DAOQLC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_27122_cov_22.122917"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..654)
                     /gene="upp"
                     /locus_tag="PL000_11435"
     CDS             complement(28..654)
                     /gene="upp"
                     /locus_tag="PL000_11435"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNC89557.1"
                     /translation="MSNVYASKHPLIAHKLSRLRDKKTEPKKFRELVREIAGLLAYEA
                     TADLATTPIEIETPLETMTAHQLKEKIGLIPILRAGLGMVEGIWELMPSAEVWHIGLY
                     RDEHTLQPVEYYNKLPVDPRVSVCLILDPMLATGGSATATADILKRWGVTKIKFVGLI
                     AAPEGIKAMQEAHPDIDIFIAAIDDHLNERAYIVPGLGDAGDRQFGTG"
     gene            790..1482
                     /locus_tag="PL000_11440"
     CDS             790..1482
                     /locus_tag="PL000_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC89558.1"
                     /translation="MTQTRREKLRDATREEILSTAWKQIAEAGASGLSLRGIAREMGM
                     TAPGLYRYYKDRDALVTVLLMDAFGSFADALETGRDACAADDHAGRFREMGRAYFRWG
                     SENPQKYALLFGSPIHGYVFAEELVPVAQKSFFVLVGVIGAAFAAGKINGEIPALKLP
                     TSLKVQYDALKKMGIPFSGGVIQLALSAWAQMHGITSLYLQNYLSGMLQDGVGAFVDF
                     EIEKMIKIFGME"
     gene            1495..2463
                     /locus_tag="PL000_11445"
     CDS             1495..2463
                     /locus_tag="PL000_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC89559.1"
                     /translation="MKVLVVGAGIIGTIFGWALAEDGHDVTHFVRKGKAGNFPHGAQI
                     DMLENRKGKKNFIGRYDIKLAEVLSPSDGFEAVIVPTKPYQLMDVLKEIVPQTGNADY
                     LLLTQNWTGTAEIDAVLPQARYIFGDAHAGGVWKDGVLIATIMDDIILGQVDRGRGAV
                     LQKFADLFTGINLKVTIPENILHYIWVQHAMTTGLWMGLVRTGSLENILKDPVNGPLS
                     LQAVKECLTVAEKRGIDLQKYPEIKMYLQTGSKIGMAITGFVVRMMFKLNKSIQRSSA
                     HGLGDPIEIRSAYEDLLKSGAEFGVPMPIMQSFRPDVERLVAIGVH"
     gene            2504..2944
                     /locus_tag="PL000_11450"
     CDS             2504..2944
                     /locus_tag="PL000_11450"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HNC89560.1"
                     /translation="MNGNDFMAWVLRSPLHGMLSGGMMLMTVTGRKTGKKYTTPVGYY
                     VEGEHLWVITSRERTWWKNLRGGATVDLLLKRKPVQGMADVELDEKSVAARMNEYLHH
                     VPQAARPMGIRVENSRPNAEDVARTAKDRLFVRIKLAEPIKNER"
     gene            complement(2915..3277)
                     /locus_tag="PL000_11455"
     CDS             complement(2915..3277)
                     /locus_tag="PL000_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89561.1"
                     /translation="MTQNTFPCIKCGGPNEPEAGQTSMACIYCGAALTIPEELRVKSM
                     PRVEKIQPKPKPAPSLEKETPELLRKAQPLAIKAWNMYAYWTWIRWVLPTCLTVLTVA
                     FFICMALGALPFVFNWFR"
     gene            3431..5404
                     /locus_tag="PL000_11460"
     CDS             3431..5404
                     /locus_tag="PL000_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC89562.1"
                     /translation="MTEQFTFGGEIVWKPSREQIEHANLTAFMKQHGIKDFNELMKRS
                     TEDVAWFTDAVLKFLDIQFYEPYSQVVDLSQGIQFPAWCVGGRMNIVLNCVDKYQSSP
                     NSGRLAMVFEGEEGITKSLTYEMLYKEVNKTANALRSLGLGKGDAVGLFMPMTPEIVI
                     ALLAIAKIGGVVLPLFSGYGAGAIVSRMTDANAKALFAADGAFRRGKAVEMKSIADEA
                     AEQIGSLQHMIVLRRTGQAIQMKAERDHWWHELVDGQSDVCETERTSAEDPLMIIYTS
                     GTTGRPKGAVHTHCGFPIKAAQDMAFGTDVHGAFRAERSDVIYWMTDMGWMMGPWQVF
                     GSLLLGATMFLYDGAPDFPAPNRLWELAEKHRINQMGVSPTLIRSLIPQGDGHFEKHD
                     LSSLKCFASTGEPWNPDPWMWLFEKVGGGRIPIINYSGGTEISGGIVMGNLLLPLKPC
                     AFSAACPGMDADVVDENGRSIRNAVGELVVKAPWIGMTRGFWNDRQRYLDTYWSRWEN
                     VWVHGDFAAIDGDGLWYILGRSDDTIKIAGKRLGPAEVESILVRHGSVVEAAAIGVPH
                     EVKGSELVLFAVTSPGVVGGDALRQELYEMVVAEMGKPLAPKAILFVSDLPKTRNAKV
                     MRRVIRSAYLGLELGDTSSLVNPQAVEEIRRAK"
     gene            5466..7235
                     /locus_tag="PL000_11465"
     CDS             5466..7235
                     /locus_tag="PL000_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC89563.1"
                     /translation="MKKARYPLGTPRYLLWVYLLILTIVFSACGSPTPITVVVTATSL
                     PPTEGVVEPTATLVPVNLSGPQSGDKIRWIDGSNLIYVPPGEFVMGNGGFDAPVHNVT
                     LDGYWMYQTKVTNRMFAQCVAVGSCTPPTEELGGPVYSNPEYANHPVVGVTWDQAQAY
                     CGWANGQLPTEAQWEKAARGLAGNLYPWGNDEPACDLVNFGYCLGRTTEVDAFPDGAS
                     DFGMYDMAGNLFEWVSDWYSETYYSEAPAANPLGPDSGQYRVIRGSSFETDLELVESA
                     IRHFGAQGYHTRDIGFRCTVPQPQLFAPYCQLSAYIPSGVIVSNGCQLPEAAVEGQYC
                     AVGRSFATVSLSPGAIYQTGEGLECEEAIVDGQRILTCVGPFAQEATNEITVCNPTCS
                     NSPDVTGATPTCDPGYTLDPTTNTCNYSPVPSELSVAGCPVGYNLVDRGGQQTCVIAT
                     NANGECPNGLYYDDLAGRCVPPNGLVEAPIGINNAPLAAQSYSGCAAGFTYSDTFQCC
                     QAVTGGTYPGCSPGSTFNRDLGACSPGEIRLAGPGCVTLDVTTLKCSQPVDVCSRIKS
                     ETTCIRNSYACKWDDEINVCTLR"
     gene            7571..8065
                     /locus_tag="PL000_11470"
     CDS             7571..8065
                     /locus_tag="PL000_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89564.1"
                     /translation="MERVEYVLLLMGAIFCAYRAIVSKRILSSTIYLACISALISVVL
                     FMLGATQVAVMELSVGAGLVTVLLVYALSVVGEDALDPSSVIPKPFAIFVVSAAALFL
                     GWMVYPAVQFDSQMASIDLASVLWQQRVLDVWIQIVLIFSGVMGVLGLLSERTPTGIT
                     GGRA"
     gene            8062..8373
                     /locus_tag="PL000_11475"
     CDS             8062..8373
                     /locus_tag="PL000_11475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HNC89565.1"
                     /translation="MNLTVLNIILLGIIGLLGVGVYGLLITRNLIKVVMVLQILVKAV
                     ILAFVFSGKVSGNLGLGQSIAATVIVADTIVAVVGLALAVQVRRRVGTLDAPQVSTLR
                     G"
     gene            8375..10129
                     /locus_tag="PL000_11480"
     CDS             8375..10129
                     /locus_tag="PL000_11480"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC89566.1"
                     /translation="MAGILILLIIGFPWLGAFVVWAAGDSRPKAQHALAVTFSLFSGI
                     SSLVLLAFVNSEPVIDLALGTSFGNLTFSPDGLAVSLTVIATVVGSLAVLFSVDYMHG
                     EAQLGRYYFLVLFFIGAMAGLVLSGNLLFTFFFWEITALCSYGLISFYNDDPKAVAGG
                     IKALIITQVGGVGLLAGALMAYANLGSFQISALLANAESIPTAALSFIAFSFLIAAAA
                     KSAQFPFHTWLPDAMEAPTPISALIHAATMVNAGIYLLARFYPAFESVPAWKITVMLV
                     GVTSALITAFMALTATDLKRTLAYSTVSQLGYMVYAIGAGGILASQFHLFSHAVFKAL
                     LFLTAGSVIHAVGTRDMRRMGGLGAKMPFARNVFIIGALALAGIPIFNGFWSKELILE
                     IGLEHSPIWAYALMLLGAGLTAFYTFRMTWMVFFGTPREALHVHSVGAAMKISLTVLS
                     IGTLTTWLLFGGLNGILSTSLPFHEIETESTLEMIKAISFAPATWFALLVVTLGLGLF
                     FIFGRKFALSESPAWFGMLTESSFGFEPVNRFVVSFVSGGAERLRATQTGQLNWNILA
                     IVGALLVVLAVLVWGGAL"
     gene            10126..11646
                     /locus_tag="PL000_11485"
     CDS             10126..11646
                     /locus_tag="PL000_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNC89567.1"
                     /translation="MNILNFVSMITIPLIATPMVYLSGRLGTRETVLHSRSYLVRGLA
                     LLAILLAWIPFVVSARAFLAGGVLEFSIESIWLRVDGISLLVAAMALTLGTLVVLFSG
                     PYMAGEVGEEKYYAMLLAMIGLMIGLACADDLFNLWVWFEGMAVSSYLLVAFYREQSA
                     SLEAGIKYLVQSAVGSVLALLGISLVLANAGTLNMPEVREAVASLSVNRFALLGAGAL
                     FVIGFGVKVAVVPLHTWLPDAHSQAPSGISAMLSGVVIEAGLIAMLRALSVLTGFSNS
                     WGLLFMIFGALNILYGNLLALRQTTIKRLLAYSSLSHIGYILLGLGVAFYSGAALGAQ
                     GAFFHFFTHMLMKGLAFLSVGALMYGMFLQNDSHAALKVTDLAGAAQKYPLVALAFSI
                     AVLALGGLPPLAGFMSKWQIFVAGFQVQNNWVTALMIFMALNSVLSLAYYAPLVNLMY
                     RKSPSEQVMAGKPLPLAINLPLLLMMLAVIVLGFVPQLMDWVTVPAAQVFIAMFGG"
     gene            11650..11964
                     /locus_tag="PL000_11490"
     CDS             11650..11964
                     /locus_tag="PL000_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89568.1"
                     /translation="MNNFILTPVFAFLAYALAVSAVSGLARMFSAKGQKSQFKSEPYA
                     SGEDYDPVPAAPGYRQFFVIALFFAVLHLGIIMVGSSDLSSVTIVYLLGLILALVALI
                     LG"
     gene            11982..12491
                     /locus_tag="PL000_11495"
     CDS             11982..12491
                     /locus_tag="PL000_11495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNC89569.1"
                     /translation="MNKNNEQFWSQIIDKVRTWARLNSIWAVHFNSGSCNGCDIEILA
                     TLTPRYDVERFGIKLEGSPRHADVLIVTGPVTRQARERLIRTYEQMPEPKFVISVGSC
                     SISGGVFEGCYNVGGSVDEVIPVDVYIPGCPPRPEAIIDGIVQLLASLKGGEPRKMAR
                     PANVEEMPA"
     gene            12488..14161
                     /locus_tag="PL000_11500"
     CDS             12488..14161
                     /locus_tag="PL000_11500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNC89570.1"
                     /translation="MNIEQTLKDAEQFLAPWAKELLHPESNRLDVAIQVDDLRAATSA
                     LLAAHWGYLSAITGTDLGVEVGQLEALYHFCNGPAITSLRIHLPREIDACLPSIADLI
                     PSASFFERELHEMMGFAFHGLDVSSRLFLPDDWPENVYPHRADFSPDRAAPVAGRRAD
                     ELPEQGVVGGNTFVIPIGPQHPALKEPGHFEFTVDGEIVTAAKMRLGYAHRGIEKAAE
                     SRNWIQNLYLFERICGICSHTHSMAYSLGVERLAQVEAPPRAQAIRELVAGLERVHSH
                     LLWLGVAAHEAGFDTLFMYSWRDRETVMNLLEQITGNRVNYSANVLGGVKCDIGAEQA
                     KAVKEGLTYLETRIRHYLEVVTTDAMFLNRTRGIGTISREEAEKFGLLGPTARASGLS
                     RDVRLEAPYGAYHHFPVSLVMETNGDLEARCVVRLKELLVSCFTMRTIVDNLPEGPLT
                     TRMPRKIPAGETVSRIEAPRGELFYYIKSKGGENPARVHIRTPSLCNWVSVLEKAVGH
                     QLADVPMLIAGMDPCFSCNDRVVTVNRPSTNETWSWKQLREHGIEYYKR"
     gene            14158..15081
                     /locus_tag="PL000_11505"
     CDS             14158..15081
                     /locus_tag="PL000_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNC89571.1"
                     /translation="MNTFSSFVTLLFFPAGLSLIVVGMFYEWVDRKLVARFQNRIGPR
                     WFQPFADMVKLFAKEQIKPAFVNPYLFFGLPVVALAGALTSALYVPLMGLTPASSFPG
                     DLIVTIYLLSLPTMCVGLAGWNNSGRFSLIGATRALTQLFAYEAPFLLALLGPALVAG
                     SWQINTINRFTQSHWLILTQPVGFIVALIGLMGKLELPPFDAPEAETEIVAGALTEYS
                     GRGLAMFKIGKSVELIVGLCLVASFYLGGLNGVPSFLFKTLILLLSMTMLQSLLTRLR
                     IDQTVGMWWRFGAILVLAQLLVIILVKGFLP"
     gene            15078..15509
                     /locus_tag="PL000_11510"
     CDS             15078..15509
                     /locus_tag="PL000_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC89572.1"
                     /translation="MKIGTMLKDIVASFFKKSSTQLYPFEKTRAPERYRGNLFYDPSA
                     CTGCCLCVKDCPSKAIELVTLDRAAKRFVLKYHMDQCIYCGQCAVNCKFKCMGMTSQD
                     WEHAALDKKEFTVYYGKDEDIKQLLENVSKPAVEPTDNQTG"
     gene            15463..15978
                     /locus_tag="PL000_11515"
     CDS             15463..15978
                     /locus_tag="PL000_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HNC89573.1"
                     /translation="MSLNQQLNQLTTKLDELPRVAVVGIGNTLRNDDGAGILVARELT
                     RREHLAGMHHVLVIQAGHAPENVTGELREFDPDLILLVDAAEFRDVPGAICWVPIQAI
                     EGMSASTHSLPLSMLARYLTLELHCDVALIGIQPKSNDVGEVISVEVQAAVNEIVNEM
                     CDACLIIKPAY"
     gene            complement(16108..16443)
                     /locus_tag="PL000_11520"
     CDS             complement(16108..16443)
                     /locus_tag="PL000_11520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC89574.1"
                     /translation="MKTQSGLEYIEVEAGTGAQAVAGKTVSVHYTGKFQDGRVFDSSV
                     SRGEPITFPLGRGNVIKGWDEGIALMKVGGKAQLIIPPHLGYGERGAGGVIPPNATLV
                     FDVELVSVQ"
     gene            16544..16981
                     /locus_tag="PL000_11525"
     CDS             16544..16981
                     /locus_tag="PL000_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC89575.1"
                     /translation="MSTVRDMIRKKGSEVFSILPEASVYDALEIMAKHNTGALMVVSG
                     GRVEGILSERDCIRKLDLEGRTAKNTKVSEIMTSKVVYVEAAQPLEECMALMIDKNIR
                     HLPVFDGKDLLGLISVRDVLKEVVDVQKFLISQLEHYITGGGR"
     gene            16983..18482
                     /locus_tag="PL000_11530"
     CDS             16983..18482
                     /locus_tag="PL000_11530"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005300.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC89576.1"
                     /translation="MQEQTISEFQKKMISGELTSRQLTELFLERINSVDHNGPKLNSV
                     IETNPDALTIASNLDEERRAGKVRSQLHGIPILIKDNIDTHDRMQTTAGSLALEGHIA
                     PRDAFIVKQLRKAGAVILGKTNLSEWANFRGRRSISGWSSRGGLTHNPYALDRSACGS
                     SSGSGAATAANLCAAAVGTETDGSVICPAQTNGIVGIKPTLGLLSRTGIIPIAHSQDT
                     AGPMARTVADAAILLGAMTGMDEKDPSTKRGSKHIAKDYSKFLDPNGLKGARIGVARN
                     MCGTDARIIKIFEASIAVMKQQGAVIVDPANLPNFNKFGATELEVLHYEFKADLNKYL
                     AGSNSKLKTMAEIIKFNEEHKSRVLKYFGQEHMLAAQEKGSLRDKKYREALAKNLLLT
                     RENGIDATLKKYKLDAIVVPSGGPAWMIDLANGDAINWDMESTSPAAVAGYPHITVPA
                     GYVFGLPVGISFFSTAWQEPTLIKLAYAFEQSTQVRKPPQFPATVFGNL"
     assembly_gap    18488..18587
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18588..20607
                     /locus_tag="PL000_11535"
     CDS             <18588..20607
                     /locus_tag="PL000_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNC89577.1"
                     /translation="FLLSSFLSKGLPLPMHLFELFEKPVGFFLVVMAIILITPLLSEK
                     LKLPGIIGIIIGGMLIGPHGFGLLEDNDRIQFLSTIGLVYLMFSAGLEVDINQFMKVR
                     TRALVFGVITFAFPQLMGMGLGYILGLDWLGMILLGSAFASHTLIAFPILTKLGVARN
                     EAVAVTTGATVLTDIGAFIVLAVVLGAKSGGGITFGYFAQLLVLLTIFTAAIIFGLPR
                     LGKLIFQKLTGRAVEFQFVIVALFVAAFTAELIGVHEVVGAFLAGLAINSMLPRHSPV
                     AGHVLFVGESFFIPIFLLYSGLITDPLTFLKSPQTIVVAIGVTIVAYVSKLVAAWITA
                     RIYKYTKSEFWTVYGLSHAQAAVTIPTLVIGLETGLFDSTLFNAAILMILLTSITSPL
                     IVQRFASDLQTASADDEQTPLFGRILVPVSDVKQSEGLVSLASLLARTSKGKVLAVSV
                     SNDKGLSNGTTSFGIHKEMLGRVTETLNDPETEIELIPRMASTHAQGILHTSHEENAS
                     LIVMGWRGRRTLRENVLGSVLDEVIWGSDTPVMVGKLPLPLNSMTRVIFVVPAASVPP
                     IALRRMLQATVVFAKSLNVPLLVRADSSYLQNLEALLVVTREDHPVTLEVLKDQLKPE
                     KLAHEAVSDFIILPGFGSRKRVADTLGNIPEQIAKSFEGNLAILHFDK"
     gene            complement(20650..21576)
                     /locus_tag="PL000_11540"
     CDS             complement(20650..21576)
                     /locus_tag="PL000_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HNC89578.1"
                     /translation="MLLLTRKEIEHLLDPDELLTVLEEGFKALSAGQLNVPPRNQAIA
                     PKGLLVGMPAYMPNQFMSVKLVTIFHENIPAHQATITLFDSNTGTPIAFMDGEHITAM
                     RTAAGAILSIKHLARKDSKSIAIVGAGVLGQAHLQMIGTLSGIEKIWIASKDISKAQA
                     LAARASNALAVDSVREAVSNADIVCLCTSSLEPVIQAEWLKKGVHVSSVGYRPPGGEL
                     PRDLVEASHVFVESKVAFQAPPVGSAELQGLNAEFGTEMGEYLLGQKPGRRNETETTI
                     YKSMGHAMEDLVTANLVYQKALAQGIGTTFDL"
     gene            complement(21603..22634)
                     /locus_tag="PL000_11545"
     CDS             complement(21603..22634)
                     /locus_tag="PL000_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89579.1"
                     /translation="MTTPLPRAIVVEDDQAWQQIMSEILSDCGLDVDVAGSLEEAIPL
                     LKAHPHRLAVADLSLSANHNNTDGLRVLENIKHLDPECKSILLTGFATVEVAVAALTR
                     HHAHTFLQKESFNRGQFKEIIHNILTSAPKPEEEKAAPAPAQPTLQNPPAMPKALIVD
                     DDAGWRSILEDLLSDLGFQPVLCASFGEAVGALKKNQLTLAIIDLSLNEAVSQNGYPL
                     LGLAQERKIPTVVISGLSEPEEIQRIYSRYSIVAFIEKHAFDRATFKRIITEAKDSPV
                     PDDKLSTLTEREREVLDLLAKGMTNKEIADQLVITTNTVKRHLKAVFEKLEVHTRSAA
                     TAKVTRGSL"
     gene            complement(22631..24796)
                     /locus_tag="PL000_11550"
     CDS             complement(22631..24796)
                     /locus_tag="PL000_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC89580.1"
                     /translation="MANSPIPPSEELNPRTLEALASLNQISTAINQITSSEDQSSSLQ
                     LIVDSAIRVVPGFSASIYIYNPNTDRLEIESRVVSYAKGYEEYRHINPNDDVPRSNGF
                     GMRAIQRKRRTLSYEEANLQIHPYQEKLGVKVVACFPLLASKEVLGALYIYLYEERQL
                     SQFEELMLHNFVNQAAMAIYHARHVAGIKHNLAQKENELNRLRHAGMLISSRMRLEET
                     LESILELALELTNAQYGIFRLVDKSGEDLVTRAVSSVHLEKPLVEKLPIKGQSVMAYV
                     ARTRQSLLIRDLREKPWNSVYYPLDSKLEMRSELCIPLINASGRLEGVLNLESPRIGG
                     FSEDDSHMLQSLATYAVTAIQEVRLLDALQEAAQLLISQSSQEVLNTLCGMANDLLNT
                     SSSAVWLANGQGEMELAASNGADFPAQDPQGMDLPNALLMPLLSGENDSAAGIFGVFQ
                     AAPGGVRSDTTEWDKKVLACLANYAVLAIQNESRQQALRSSQEQHLIAETFAAVGDIS
                     ANLLHNMNNKVGTIPVRVQAIQDKYDTLLESDAYLNKSLIEIERSAMAAMQIVRENLS
                     HLRPIRIEQIQVAPRVQDAIHTIQVQPGTRIIISGLDALPTINANAQSLTFVFRNLIE
                     NAMDAMKGNGTISVSGMVIEKNVEVRVADTGPGIKPELQEQIFELEYSSRSHPGKLGF
                     GLWWVRTLMTRLGGSVQVESDGEHGTTFVLKLPIAEVRA"
     gene            complement(24865..25494)
                     /locus_tag="PL000_11555"
     CDS             complement(24865..25494)
                     /locus_tag="PL000_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNC89581.1"
                     /translation="MHISLFISSFILAISFCAPPGVVTAETFRRGSARGFFPALYVQF
                     GSLIGDTTWAVIALTGLAFVIQNNIARTILSLIGIALMLKLAWDALKDARHGNGLNIS
                     EPASSRGDFASGAFLSLGNPMNIVFWTGLGTTVFASIAGGPEPIHFVTFFAGFLSGAV
                     LWCFILAGLIAWGRQWMTVGFFRFLNATCGLVLFYFAFQLGIQTMQNFI"
     gene            complement(25589..26995)
                     /gene="glnA"
                     /locus_tag="PL000_11560"
     CDS             complement(25589..26995)
                     /gene="glnA"
                     /locus_tag="PL000_11560"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNC89582.1"
                     /translation="MAKSIKEVLEMAKSAQVVDLRFTDLPGTWQHFTMPVHRLTEDFF
                     KEGIPFDGSSIRGFQEIHESDMLLKADPDTAFLDPTAETPTLVLSCNVFDPITEEAYS
                     RDPRYVAQKAEAYAKSTGLADTVYFAPEAEFFVFDGIRYVSTPHEASFSIDSEEGWWS
                     SNREGNKGGQIQPKRGYFPVSPTDTLQELRNKFMLAMQAAGIQMDLHHHEVATAGQSE
                     MSMTFTTLTRMADDLLLYKYIIKNVARKYGKTVTFMPKPIFGDNGSGMHVHSSMWKDG
                     KNVFFDEKGYAGLSQTAKYYIGGLLKHAPALLALTSPTTNSYRRLVPGYEAPVNIAYS
                     QRNRSAICRIPATKSWKAKRVEFRAPDATANPYLAFSAVLMAGIDGILNKIDPGEPKD
                     VDLYELPAAEKALIKQVPSSLDAVLHALEADHEFLLKGGVFTPDLLEEYIKYKRTHEV
                     DAIRLRPTPHEYVLYFDA"
BASE COUNT         5763 a         6643 c         7688 g         6928 t
ORIGIN      
        1 aaaaatggac ttatgcaaga gttctattta tcccgtgcca aactggcggt cacctgcatc
       61 tccaagcccg ggcacaatgt aagctcgttc gttcaggtga tcgtcaatgg cggcaatgaa
      121 aatatcaatg tcaggatgtg cctcttgcat ggctttgatt ccttcggggg cggcgatcag
      181 gccgacgaat ttgatcttgg tcacacccca gcgtttgagg atatcggctg tggctgtggc
      241 tgaaccgcct gttgcgagca tggggtcaag aataagacag accgaaacac gcggatcgac
      301 gggaagtttg ttgtaatact caacgggctg caaggtatgt tcgtcacgat acaagccgat
      361 atgccagacc tcggcgctgg gcatgagctc ccaaatccct tccaccatgc caagaccggc
      421 gcgcaaaatg ggaataagac cgatcttttc ctttaactga tgggctgtca ttgtttcgag
      481 cggggtttcg atctcgatgg gtgtagtggc caggtcggca gtggcttcat atgcaagaag
      541 tcccgcaatc tctctaacga gttcgcggaa cttcttgggt tcggtttttt tatctctcaa
      601 gcgtgaaagt ttgtgggcga tcagtgggtg tttggacgca tacacgtttg acatgacatt
      661 ctccttggag tgagttggcg caattatagc gtgagaaagg cagcctgttt gctcttgaca
      721 ggggtgagat ttttgctaaa atagtaaacg ctgtaaacaa aaataatcac atgtaacaaa
      781 aaggcagcca tgacacaaac acgaagggaa aaattacgcg atgcaacccg tgaggagatt
      841 ctctccactg catggaaaca gatcgctgaa gcaggcgctt cgggactttc cctgcgcggg
      901 atcgcccgcg aaatggggat gacggcgccg gggctgtatc gctactacaa agaccgtgat
      961 gcactggtca cagtgttgtt gatggatgca tttggttcgt ttgccgatgc gcttgagact
     1021 ggccgtgatg cctgtgccgc cgatgatcac gccggacgct tccgtgaaat gggcagggca
     1081 tattttcgct gggggagtga aaatcctcag aagtatgcat tgctttttgg cagcccgatc
     1141 catgggtatg tttttgcaga ggaattggtg ccagtggcgc agaaaagttt ttttgtgctg
     1201 gtgggcgtca ttggtgcggc gtttgcagca gggaagatca acggggagat accagccctt
     1261 aaactgccga ccagtctcaa ggtccagtat gacgcgttga agaagatggg aatcccgttt
     1321 tcaggcggcg tcatccagtt ggctttatct gcctgggcgc agatgcacgg tatcacgtcg
     1381 ttgtatttgc aaaactatct ttctgggatg cttcaggatg gcgtgggcgc gttcgttgat
     1441 tttgagatcg aaaaaatgat caagattttt ggaatggaat aaaaaggaga aggcatgaaa
     1501 gtgttggttg tgggagcggg aatcattgga acgatctttg ggtgggcact ggcggaggat
     1561 gggcatgacg tgacgcattt tgttcgcaaa ggcaaggcgg ggaattttcc gcatggtgcg
     1621 cagattgata tgcttgaaaa tcgcaaaggc aaaaagaatt tcattggcag gtatgacatc
     1681 aaacttgctg aggttttgtc tccttcagac ggatttgagg cagtgatcgt accgaccaag
     1741 ccgtatcaat tgatggatgt tttgaaggag atcgtgccac agactgggaa tgcagattac
     1801 ctgttgctga cccagaactg gaccggtacg gcagagatcg atgcagtgct tccccaagcg
     1861 cgatatattt ttggcgatgc acatgcaggt ggggtatgga aagacggcgt tttgatcgcc
     1921 accattatgg acgatattat tttgggacag gtggatcgtg ggcgtggtgc ggtattgcaa
     1981 aagttcgccg atctattcac tggaatcaat ttgaaagtga ccatcccgga aaacattttg
     2041 cattacatct gggtacagca tgccatgacc acaggcttgt ggatggggtt ggtccgcacg
     2101 ggtagtttgg aaaatatttt gaaggacccc gtcaatgggc cgttgagttt gcaggctgtg
     2161 aaggaatgtt tgaccgttgc cgaaaagcgc ggcatcgacc tgcaaaaata tcccgaaatc
     2221 aaaatgtatt tgcagaccgg ctcaaagatc ggcatggcca ttacgggttt tgtggtgcgg
     2281 atgatgttca agctcaataa aagcattcag cgttcgtcgg cacatggttt gggtgatccc
     2341 attgagatcc gctcggcata tgaagacctg ctgaaatcgg gtgcggaatt tggtgtgccg
     2401 atgccgatca tgcagagctt ccgccctgat gtggaaagac ttgttgcgat tggggtacat
     2461 tagttttccg cggtgcggtt gaaatggcag gaaggagtta tgaatgaacg gaaatgattt
     2521 tatggcctgg gtgttgcgtt cgccgttgca tggcatgtta agtggcggca tgatgctcat
     2581 gaccgttaca gggcgcaaga ctggtaaaaa atatacaacc cctgttggct attatgtgga
     2641 aggcgaacat ctgtgggtca ttaccagccg agagcggacg tggtggaaga atctgcgagg
     2701 cggcgcaacg gttgacctgt tgttgaagcg aaagcctgtg cagggcatgg ccgatgttga
     2761 actcgatgaa aaatctgtag cggcgcggat gaacgagtat ctgcatcatg ttccgcaggc
     2821 ggccaggcca atgggcatcc gcgtcgaaaa tagcagaccg aatgcagaag atgttgcccg
     2881 tacagcaaaa gatagattgt tcgtaagaat taaactagcg gaaccaatta aaaacgaacg
     2941 gtaatgcacc caacgccatg caaatgaaaa aagcgacggt aagaaccgta agacaggtgg
     3001 gaagcaccca gcggatccac gtccagtagg cgtacatatt ccaggctttg atggcaaggg
     3061 gttgtgcctt gcgaagaagt tcgggcgttt ctttttccaa actgggagct ggttttggct
     3121 tcggctgaat tttctccacc cggggcatgg actttacccg cagctcttcg gggatggtaa
     3181 gcgcggcgcc gcaatagatg caagccatgc tggtctgtcc tgcttcgggc tcgtttggcc
     3241 cgccgcattt aatgcaggga aatgtgtttt gtgtcattga attaggttct ccagatcggg
     3301 gcaattatat aatacccacg gtcacaaatt gcgctcctct cttttctcaa aaagcgcaat
     3361 ttgtgaccga atgcggtata aaagcaagtt gcgatagaat cagcacaacc ccaaccagag
     3421 gagaatatcc atgaccgagc aattcacatt cggcggtgag atcgtttgga agccatcccg
     3481 tgaacagatc gaacatgcaa acctgaccgc tttcatgaag cagcatggaa ttaaagactt
     3541 caacgaatta atgaaaaggt caacggagga tgtcgcctgg ttcaccgatg cagtgctgaa
     3601 gtttcttgat attcaatttt acgaaccata ttctcaagtg gttgatctct cgcagggaat
     3661 ccaattccct gcatggtgtg tgggcgggag aatgaacatc gtcctcaact gtgtggataa
     3721 atatcagtca tcgccaaaca gcggacgact ggccatggtg tttgaggggg aagagggaat
     3781 tacaaagagc cttacctatg aaatgctcta caaggaagtg aataagactg ccaatgcttt
     3841 gcggtcattg gggcttggca aaggagatgc ggttgggctg ttcatgccga tgactcctga
     3901 aattgtcatt gctctgcttg ccattgcaaa gatcggaggc gttgtcctgc cgttgttttc
     3961 gggttatgga gcaggagcca tcgtctcacg catgacagat gcaaatgcga aggcgctttt
     4021 tgcggcagat ggcgcttttc gacgcggcaa ggctgtggaa atgaagtcca ttgcggatga
     4081 ggcggcggaa caaatcggct cactgcagca tatgatcgta ttgaggcgga cgggtcaggc
     4141 aatacagatg aaggccgaaa gagatcactg gtggcatgag ttggtggatg ggcaaagtga
     4201 cgtttgcgaa actgaacgga catctgctga agatccgctg atgatcattt acacttcagg
     4261 gacgacgggc aggccaaagg gcgctgttca tacccattgt ggatttccga tcaaagccgc
     4321 gcaggatatg gcttttggga cggatgtgca tggcgcattt cgtgctgaac gctccgatgt
     4381 gatctactgg atgaccgata tgggctggat gatggggccg tggcaggtgt ttggaagtct
     4441 gctgttgggg gcgacgatgt tcctttatga tggcgcacct gatttccctg ccccgaaccg
     4501 cttgtgggaa ctggctgaaa agcatcgcat caaccaaatg ggagtttcgc ccactttgat
     4561 ccgctctttg atcccgcagg gagatgggca ttttgaaaag cacgatcttt catccttgaa
     4621 gtgctttgct tccacaggcg agccgtggaa tccagacccg tggatgtggc tgtttgagaa
     4681 ggtgggaggc gggcgcatcc cgatcattaa ttattcgggc ggcaccgaga tctcaggcgg
     4741 aatcgtgatg gggaatctgc ttttgccttt aaaaccatgt gcgttttcgg cggcctgccc
     4801 cggtatggat gcggatgtgg tggatgaaaa tggaaggtcc attcgcaatg cagtgggaga
     4861 gctggtcgtc aaagcgccgt ggatcggcat gacgcgtggt ttttggaatg acaggcagcg
     4921 ctatctcgat acctactggt cgcgctggga aaatgtgtgg gtccatggag attttgccgc
     4981 gattgatggc gacgggcttt ggtacatcct cgggcgctcg gacgatacga tcaagatcgc
     5041 ggggaagcgg ctggggcctg ccgaggtgga gtcgatcctt gtgaggcatg gttcagtggt
     5101 ggaggcggcg gcgatcgggg tgccgcacga ggtcaagggg agtgaattgg tccttttcgc
     5161 agtgaccagc cccggggtcg ttggaggcga tgctcttcgt caggaattat atgagatggt
     5221 ggtcgctgaa atgggcaagc cgctggcgcc gaaggccatc ctctttgttt ctgacctgcc
     5281 caaaactcgg aatgccaagg tgatgcggcg ggtgattcgg tcggcgtacc ttggtttgga
     5341 gttgggagat acctcgtcac tggtcaatcc acaggcggtg gaggagattc ggcgcgcaaa
     5401 atgatttttt gaccctgcat tataataacg ggcgccccgc agaacttgcg ggagaaggag
     5461 atttaatgaa aaaagctcgt tacccattgg gtacaccgcg ttatttgctc tgggtatatc
     5521 ttttgatcct gaccattgtg ttttcggcct gcggatctcc aacgccgatc acggtggtgg
     5581 tgacggctac ctccctgcct ccgaccgagg gagtggttga gccgaccgca actttagtgc
     5641 ctgtgaacct ttccggcccg caaagcggcg acaaaatacg ctggattgat ggaagcaacc
     5701 tgatctatgt gccgcccggt gaatttgtga tggggaacgg tggtttcgat gccccggttc
     5761 acaatgtgac cctggatggg tactggatgt atcagaccaa ggtgaccaat cgtatgttcg
     5821 cacagtgtgt ggcggttggt tcctgcacac cgcccaccga ggaattgggc ggccctgtgt
     5881 atagcaaccc cgaatatgcc aatcaccctg ttgtgggtgt gacctgggat caagcgcagg
     5941 cttattgcgg ctgggccaat ggccagttgc cgaccgaggc acaatgggaa aaagcggccc
     6001 gcggccttgc tggtaattta tatccgtggg gcaatgacga acccgcctgt gaccttgtga
     6061 attttggcta ctgtttgggc cgcaccaccg aagtggatgc gttccctgac ggtgccagtg
     6121 attttggaat gtatgacatg gcggggaatc tcttcgagtg ggtgagcgat tggtatagtg
     6181 agacctacta cagcgaagca cccgccgcaa atccgcttgg acctgacagc ggccagtatc
     6241 gcgtgattcg tggaagttcg ttcgaaacag accttgaact ggtcgagtct gcgattcggc
     6301 atttcggcgc tcagggttat cacactcgcg atattggttt tcgttgcaca gttccgcagc
     6361 cgcagttgtt tgctccatac tgtcagcttt cggcgtacat tccctcgggg gtgatcgttt
     6421 cgaacgggtg tcagttacct gaagcagcgg tggaaggtca gtactgcgcc gtaggccgta
     6481 gttttgccac agtgagcctt tcacctggag ccatttacca gacgggcgaa ggtttggaat
     6541 gcgaagaggc catcgtggat ggtcaacgta ttttgacctg tgtggggccg tttgcacagg
     6601 aagcgaccaa tgagatcacg gtttgtaacc ccacctgcag caactctcca gatgtcactg
     6661 gggccacgcc aacttgtgac cctggttata ccctcgaccc aaccaccaat acttgtaact
     6721 attccccggt tcccagtgaa ttaagtgtgg cgggatgccc cgtcggctac aatctggtgg
     6781 atcgcggcgg tcagcagact tgtgtgatcg caacaaatgc caacggtgaa tgtccgaatg
     6841 gtttgtatta cgatgacctc gccgggcgat gtgttccgcc aaatgggttg gtggaagcgc
     6901 cgattggtat caataacgcc ccgttggcgg ctcaatcata ttcgggctgt gcggctggat
     6961 ttacctacag tgacacgttc cagtgctgtc aggctgtgac cggtggaacc tacccaggct
     7021 gctctcctgg ctccacgttc aatcgggacc tcggagcctg ttcgccaggc gagatcaggc
     7081 ttgccggccc gggctgtgtc acactggatg tgaccacgct caaatgcagc caacccgtgg
     7141 acgtatgttc ccgcatcaaa tctgagacca cctgtattcg taattcctat gcctgtaaat
     7201 gggatgacga gatcaacgtc tgtaccttgc ggtaaatgaa gtttacccaa caccccgtgg
     7261 aatttttttc cacggggtgt tttttttatt gcggaagttt agtagatttt ctctgcgctc
     7321 tccgtgcctc tgcggtaaaa cggatttgat cgaaaacatg catccgtaac cctcccacaa
     7381 aaaataccca aaaacataaa aagcagacga tttagccgtc atttccgaat cctacaagcg
     7441 gtgactaacg gtatggtggg cgggtgatta acggtactga tgcccttttt tgtttgtggt
     7501 atatttcaca attgaggata gtatgcggag gtagagtatt tgcaggctag gttggaggcc
     7561 gtagtaaaaa atggaaagag tagagtatgt gttgctttta atgggggcca tcttttgcgc
     7621 gtatcgcgcc atcgtatcaa aacggattct ttcgtcaacg atatatctgg cgtgcattag
     7681 tgcgttgatc tcggttgtgt tgttcatgtt gggcgcaacc caggtggctg tgatggaact
     7741 ttctgtgggg gcggggttgg tcacggtgct gttggtgtac gcattgagcg tggtgggtga
     7801 agatgccctc gatcccagtt cggtcatccc aaaaccgttc gcaatttttg tggtctcggc
     7861 agcagccctg ttccttggct ggatggtgta ccccgctgtt caattcgata gtcagatggc
     7921 ttcgatcgac cttgccagtg tactctggca gcagcgcgta ttggatgtgt ggattcagat
     7981 cgtgctgatc ttttctggtg tgatgggggt gctcggtctg ctttctgagc gaacaccaac
     8041 tggaattaca ggaggccgcg catgaatctg accgtactca acatcatcct gcttggcatc
     8101 attggtctgc ttggcgtcgg ggtgtatgga cttttgatca cccgcaatct catcaaagtg
     8161 gtgatggtct tgcagatcct ggtcaaggcc gtcattctgg cctttgtttt ttctggcaaa
     8221 gtcagcggca acctcgggct gggacaatcc atcgcggcaa cggtcatcgt tgccgatacc
     8281 attgtggcag tcgttgggct ggcattggcg gttcaagtcc gtcgtcgagt tggcaccctt
     8341 gatgcccctc aggtttccac attgagagga tgaaatggct ggcatcttaa ttctcctcat
     8401 tattggcttt ccgtggctgg gtgctttcgt tgtgtgggcc gcaggtgact cacgccccaa
     8461 agcccagcat gcactggctg tgaccttcag cctgttttcg ggcatttcct ccctggtcct
     8521 tttggcattt gtcaattcag aacccgttat tgaccttgcg cttggaacct cattcggcaa
     8581 cctcaccttc tcccccgatg gtctcgctgt ttcattgacc gtcatcgcca ctgttgtagg
     8641 ttcgctggca gttctattct cggtggatta catgcacggt gaagcccaac ttggacgata
     8701 ttatttcctt gtgctgttct tcatcggtgc catggctggc ctggtcttga gcggaaattt
     8761 gctcttcacc ttctttttct gggagatcac ggcactgtgt tcgtatgggt tgatctcttt
     8821 ctacaatgat gatcccaaag ctgtggcggg cggcatcaaa gccttgatca tcacccaggt
     8881 gggcggtgtg gggttgcttg cgggtgcatt gatggcgtat gccaatctcg gcagttttca
     8941 aatttcagcc ctgcttgcaa acgcagagtc gattcccact gcggcgctct ctttcatcgc
     9001 gtttagcttt ttgatcgccg ccgctgcaaa atcggcgcaa ttcccgttcc acacctggct
     9061 gcctgatgcc atggaagcgc cgaccccgat cagcgcattg atccatgcgg ccaccatggt
     9121 gaatgcgggt atttatttgc tggctcgttt ttaccccgct tttgaatccg ttcccgcatg
     9181 gaagatcacc gtcatgctgg ttggtgtcac ttcggcgctt atcacagcct tcatggcgct
     9241 gaccgccacc gacctcaaac gcacgctggc ttattccacg gtcagccagc ttgggtatat
     9301 ggtctatgcc atcggcgcag gcggtatatt ggcaagtcag tttcatctat ttagccatgc
     9361 ggttttcaag gccttgctct tcctcaccgc aggctcggtc attcacgctg taggcacccg
     9421 tgacatgcgc cgtatgggcg gccttggcgc aaagatgcca ttcgcgcgca atgtctttat
     9481 catcggtgcg ttggcattgg caggcatccc catctttaac ggtttttgga gcaaggaact
     9541 catccttgag atcggccttg aacattctcc gatctgggct tatgccctca tgctgcttgg
     9601 cgcaggcctc accgctttct ataccttccg catgacctgg atggtcttct ttggcacgcc
     9661 gcgcgaagca ttgcatgttc attccgttgg tgccgccatg aagatctcat taaccgtgtt
     9721 atccatcggc acactcacca cctggcttct ctttggcgga ttgaatggaa tcttatccac
     9781 gtccctgcct tttcatgaga tcgaaacaga atccaccctg gagatgatca aagcgatcag
     9841 cttcgcaccc gcaacctggt tcgctcttct ggtagtgact ctcggcctgg gcctcttttt
     9901 catcttcggg cgaaagtttg cactttccga aagccctgcc tggttcggta tgttgaccga
     9961 atcttcgttc ggatttgagc ccgtcaatcg ttttgtcgtc agctttgttt caggcggcgc
    10021 tgagagattg cgcgccacgc aaacaggcca gctaaattgg aacatcctcg ccattgttgg
    10081 agcgttgctg gttgtgctgg cggttttggt atggggggga gccttatgaa cattttgaat
    10141 tttgtctcga tgatcacgat ccccttgatc gccacgccga tggtgtactt gtcgggaagg
    10201 ctgggaacac gcgaaaccgt tcttcacagc cgctcctatc ttgtgcgtgg actggccctg
    10261 ctggcaattt tgcttgcgtg gattccgttt gtggtttcgg cccgtgcctt tcttgctggc
    10321 ggtgttcttg aatttagcat tgaatccatc tggctgcgtg tggatgggat cagcctgttg
    10381 gtggcggcca tggcactgac cctgggcacg ctggtggttt tgttctcagg tccctacatg
    10441 gctggggaag tgggcgaaga gaaatattac gccatgcttc tggcgatgat cggtttgatg
    10501 atcggtctgg cctgcgccga tgacctgttc aacctgtggg tgtggttcga aggaatggcg
    10561 gtttcgtcct atttgttggt ggctttttat cgtgaacaat ccgcctctct tgaggcgggt
    10621 atcaagtacc ttgtgcagag tgcggtgggg tcggtgctgg cattattggg tatttcactg
    10681 gttttggcca atgccgggac gttgaatatg cccgaggttc gagaggcagt tgccagctta
    10741 agtgtgaatc gttttgcttt gcttggtgcg ggcgcattgt ttgtcatcgg cttcggggtg
    10801 aaggtggcag tcgttccgct tcatacctgg ctgcccgatg cgcattcgca ggcgccgagt
    10861 ggaatcagtg cgatgctttc gggtgtggtc atcgaagctg ggttgatcgc gatgctgcgc
    10921 gcactttccg tgctgacggg attttcgaac tcctggggcc ttttgttcat gatctttggc
    10981 gctttgaaca ttttgtacgg caacctgctg gcattgcggc agacaacgat aaaacgcctg
    11041 ctggcctatt cgagcctgag tcacattggt tacattttgt tggggctggg tgtggctttt
    11101 tattcgggcg cagcacttgg ggcgcagggt gcgttctttc attttttcac gcatatgttg
    11161 atgaagggtc tggcctttct ttctgtgggc gcgctgatgt atggcatgtt cctgcaaaat
    11221 gactcacatg ccgctctgaa agtgaccgac cttgccgggg ccgcacaaaa atatccgctg
    11281 gttgcgctgg ctttcagcat tgccgtgctg gccttgggcg gcttgccgcc gttggcgggt
    11341 ttcatgtcga aatggcagat ttttgtggcg gggtttcagg ttcaaaataa ttgggtcact
    11401 gcgttgatga tttttatggc gctcaacagt gtgctttcac tggcatatta cgcgccgttg
    11461 gtgaatttga tgtatcgcaa gtcgccttcc gaacaggtga tggcgggcaa gcctttgccg
    11521 ctggcgatca acctgccgtt gttgctcatg atgcttgcag tgattgtgct tggcttcgtt
    11581 ccgcagttga tggattgggt cactgtccct gcggcgcagg tatttattgc gatgtttgga
    11641 ggataggacg tgaataattt tattctcaca cccgtgtttg ccttcctcgc ctacgctttg
    11701 gcggtttctg cagtttcagg cctggcgcgc atgttttcgg caaaagggca gaaatcacaa
    11761 ttcaagtcgg agccttatgc cagtggtgaa gattatgacc ctgtgcctgc cgccccgggc
    11821 tatcgtcagt tttttgtgat cgccttgttc tttgcggttt tgcatttggg aatcatcatg
    11881 gtcggcagca gtgacctttc ttctgtcacc atcgtttatt tgctggggtt gatcctggcg
    11941 ctggtggcct tgattctcgg gtaaaggttt gaagggaaac catgaacaaa aacaacgaac
    12001 aattctggag tcagatcatt gataaagtgc gcacctgggc gcggttgaat tcgatttggg
    12061 ctgtgcattt caacagcgga tcgtgcaacg gctgcgatat cgagatcctt gccacgctga
    12121 ccccgcgcta cgatgtggaa cgcttcggca tcaaactgga gggcagtcct cgtcatgctg
    12181 acgtgcttat cgtgactgga ccagttacac gtcaggcaag ggagcgtttg atccgcacct
    12241 atgagcagat gcccgagccg aagttcgtca tttcggttgg gtcgtgcagc atttcaggcg
    12301 gcgtcttcga aggttgttac aacgtgggcg gttctgtgga tgaggtcatt ccagtggatg
    12361 tgtacatccc cggttgcccg ccgcgccctg aggcgatcat tgatggcatt gtgcagctgc
    12421 ttgccagttt gaagggcggc gaaccgcgca agatggcgag gcctgccaac gtagaggaga
    12481 tgcctgcatg aatatcgaac aaactttgaa ggatgccgaa caatttctcg ctccatgggc
    12541 aaaggaattg cttcacccgg agtcaaaccg tttggatgtg gcgattcaag tggatgacct
    12601 gcgtgccgcc acgagcgctt tgctggccgc tcattggggt tatttatcgg cgatcactgg
    12661 gacggatctt ggcgttgagg ttggtcagtt ggaggcgctt taccattttt gcaacggacc
    12721 tgcgatcaca tcgctgcgaa ttcatttgcc tcgtgagatc gatgcctgcc tgccaagcat
    12781 cgcggatctc atcccatcgg cttcattttt tgaacgtgaa ttgcacgaga tgatggggtt
    12841 cgcattccac ggattggatg tgagcagccg cctgtttttg ccagatgact ggcctgaaaa
    12901 tgtgtacccg catcgggcgg atttttcacc cgatcgtgcc gcgccggtgg ctggtcgccg
    12961 cgccgacgaa ttgcccgagc agggagtggt cggcgggaat accttcgtca ttcccattgg
    13021 cccgcagcat ccagcgctta aggaacctgg tcactttgaa tttacggtgg atggagaaat
    13081 tgtcaccgcc gccaaaatgc ggcttggata tgcccaccgt ggaattgaaa aagccgccga
    13141 aagccgtaac tggattcaga acctgtatct ctttgaaaga atttgcggaa tctgttcaca
    13201 cacccattcc atggcttaca gcctcggcgt ggaaaggctg gcgcaggtgg aagctccgcc
    13261 gcgcgcgcaa gccatccgag agttggtggc aggcttggag cgcgttcaca gccatttgtt
    13321 gtggctgggc gtggccgcac acgaagcagg ttttgatacg ttgttcatgt attcgtggcg
    13381 tgaccgcgag acggtgatga atctgcttga gcagatcacg ggcaaccgcg tcaattattc
    13441 cgcgaacgtg ttgggcggcg tgaagtgtga cattggcgca gagcaggcca aagccgtcaa
    13501 agagggactg acctatcttg aaacccgcat ccgccattat ctggaagtgg tcaccaccga
    13561 tgcaatgttc ctgaatcgaa cacgcggcat tggcaccatc tctcgggaag aagcggaaaa
    13621 attcggcctg cttggcccca ctgcacgcgc ttccggtctg tcgcgcgatg tgcgccttga
    13681 agcgccttac ggtgcgtatc atcatttccc ggtcagcctt gtgatggaaa ccaatggcga
    13741 tctggaagcc cgctgtgttg tccgtttgaa ggaattgctg gtctcatgct ttaccatgcg
    13801 gacgatcgtg gataacctgc cagaaggtcc gctgacaact cgtatgccgc gcaagattcc
    13861 cgccggagaa acggtttccc gcatcgaagc gccgcgtgga gaattgttct actacatcaa
    13921 gagcaaaggc ggcgaaaacc ccgcacgggt tcacatccgc actcccagct tgtgtaattg
    13981 ggtttcagtg ctcgaaaaag ctgtggggca tcaactggcc gatgtgccga tgttgatcgc
    14041 tggcatggac ccatgttttt cgtgcaatga ccgtgtggtc acagtcaatc gtcccagcac
    14101 caatgaaacc tggtcgtgga aacaattgcg cgaacatgga atcgaatatt acaaacgatg
    14161 aacacatttt cctccttcgt tacccttctg tttttccccg ccgggctgtc gctgatcgta
    14221 gtcggcatgt tctatgaatg ggttgaccgc aaactggtgg cccgttttca aaaccgcatt
    14281 ggcccgcgct ggtttcagcc ttttgccgac atggtcaaat tgtttgcgaa ggaacagatc
    14341 aaacctgctt ttgtgaaccc gtatctgttc tttggcctgc ctgtggttgc cctggctggg
    14401 gcgctgactt ccgccttgta tgtgccatta atgggattaa cacccgcttc cagtttcccc
    14461 ggtgatctga tcgtgacgat ttacctgctc agcctgccga ccatgtgtgt gggtttggct
    14521 ggctggaaca acagcggacg cttctcgttg atcggcgcca cacgcgcatt gacccagttg
    14581 tttgcctacg aagcgccgtt tttactggcc ctgcttgggc ctgctttggt ggctggcagt
    14641 tggcagatca acaccatcaa ccgctttacg caaagccatt ggcttatcct cacccagcct
    14701 gttgggttca ttgtggcatt gatcggtttg atgggcaaac ttgaactccc tccctttgat
    14761 gcccccgaag cagagactga gatcgtcgct ggcgcactga ccgagtacag cgggcgcggc
    14821 cttgccatgt tcaagatcgg taaatcggtg gagttgatcg ttggtttgtg cctggtggct
    14881 tcgttctatc tgggcgggtt gaatggcgtc ccatccttcc tgttcaaaac gctgatcctg
    14941 cttttgagta tgaccatgct tcagtcattg ttgacccgtt tgcgcatcga ccagaccgtt
    15001 gggatgtggt ggcggtttgg ggcgatcctg gtgttggcgc agcttcttgt catcatttta
    15061 gtgaagggat ttctgccatg aagatcggaa ccatgctcaa ggatattgtt gcatcgtttt
    15121 tcaaaaagag cagcacccag ctttatcctt tcgaaaagac ccgcgcccct gagcgctacc
    15181 gcggcaacct gttctacgat ccatctgctt gcacggggtg ttgcctgtgt gtgaaggatt
    15241 gtccatcgaa agccattgag ctggtcacgc ttgaccgtgc tgccaagcga tttgtgttga
    15301 aatatcacat ggatcagtgc atttactgcg gtcaatgcgc tgtgaattgc aagttcaaat
    15361 gcatggggat gaccagtcag gattgggaac acgcggccct cgataaaaag gagtttacgg
    15421 tttactatgg aaaagatgaa gatatcaaac agctcttgga aaatgtctct aaaccagcag
    15481 ttgaaccaac tgacaaccaa actggatgaa ttgccgcgcg tggcggtggt tgggatcgga
    15541 aatacgctgc gcaatgacga tggcgctggg attctcgttg cccgagaatt aacccgtcgt
    15601 gagcatttgg cggggatgca tcatgtgctt gtgattcaag caggccatgc ccccgaaaat
    15661 gtgacaggcg aactgcgcga atttgatccc gacctgattt tgttggtgga tgccgccgaa
    15721 tttcgggatg tgcctggcgc gatctgctgg gtgccgattc aagccatcga aggaatgagc
    15781 gcttccaccc atagcctgcc gctttccatg ctggcccgct acctgaccct ggaacttcat
    15841 tgcgatgtgg ccctgatcgg tattcaaccg aaatcgaacg atgtcggcga ggtcataagt
    15901 gtagaggttc aagctgccgt gaatgagatc gtcaacgaaa tgtgtgatgc ctgccttatc
    15961 ataaaaccag cctattgatc gtaggccagg ttttggtctt gaagaaaagc ccgccctgca
    16021 aaaagcagcc gatgcaaatt attttgcatc ggctgttctc gtttcaaatt cggtttagaa
    16081 aattcgtgta attcgcggcg aattttttta ctgcaccgaa accaattcca catcaaagac
    16141 cagggtggca ttgggaggga tgaccccgcc agcgcctctt tcaccgtacc ccaggtgggg
    16201 cggaatgatc aactgcgcct tgccgccgac cttcatcagc gcgatgcctt catcccagcc
    16261 tttgatgacg ttgccgcgtc ccagcggaaa tgtgatcggc tctccgcgtg aaacggacga
    16321 atcgaacaca cgcccatcct gaaacttgcc tgtgtaatgc acactgacgg tttttcccgc
    16381 cacggcctgt gcgcctgtcc ctgcttcaac ttcaatgtat tccaatccac tttgtgtttt
    16441 catgattctc ctgaagaatg aaattttttc gcaattgtac ccgttcttgt caggttatgg
    16501 atttcagcct atactttgaa aaaaatcagc cgaggagatg tctatgtcta cagtgcgcga
    16561 tatgatccgt aaaaaaggga gcgaggtttt ttccatcctc cccgaagcaa gcgtgtacga
    16621 tgcactcgaa atcatggcaa aacataacac aggcgcgttg atggtggtaa gtggcggcag
    16681 ggtggagggc attctttctg agcgtgactg catccgcaag ctggatctgg aaggcaggac
    16741 cgccaagaac accaaagtca gcgagatcat gacctcgaag gtggtgtatg tggaagccgc
    16801 ccagccgctc gaagaatgca tggcgctgat gatcgacaaa aacatccgcc atctgcctgt
    16861 ctttgatggc aaggatttgc tcggcctcat ctctgtccgc gacgttttga aagaggtggt
    16921 ggacgtgcag aagttcctca tctcccaact cgaacactac atcactggcg gcgggcgctg
    16981 atatgcaaga acagaccatt tcagaatttc aaaagaaaat gatctcaggt gagttgactt
    17041 cgcgccaact caccgaattg tttctggagc ggatcaactc tgttgaccac aacggcccca
    17101 aactgaattc cgtcattgaa accaacccag acgccctgac catcgcttca aaccttgacg
    17161 aagagcggcg tgctggcaaa gtacggagtc agctccacgg cattcccatc ctgatcaaag
    17221 acaacatcga cacccatgac cgaatgcaaa ccacagcagg ttcgcttgcc ttggaaggtc
    17281 acatcgcccc acgcgatgcc ttcattgtca agcagcttcg caaggctggc gcggtcattc
    17341 tgggtaaaac caatttgagc gagtgggcta attttcgcgg caggcgttcc atcagcggat
    17401 ggtcttctcg cggcggcctg acacacaatc cctatgcgtt ggatcgctcc gcttgcgggt
    17461 catcttctgg ttcaggcgca gcgacggcgg caaacctttg tgcggcggca gtcggcaccg
    17521 aaaccgatgg ttcggtcatt tgccctgctc aaacgaatgg cattgtggga atcaaaccca
    17581 cgctgggcct gttgagccgc actgggatca ttcccatcgc gcacagtcag gatacggctg
    17641 gccccatggc aaggactgtt gcagatgcgg cgattcttct cggggccatg acaggcatgg
    17701 atgagaaaga tccgtccaca aagcgtggat caaagcatat cgcgaaggac tattccaaat
    17761 tcctcgatcc caacgggttg aaaggtgcgc gcatcggtgt ggcgagaaat atgtgtggga
    17821 ccgatgcacg catcatcaaa atttttgaag ccagcatcgc ggtgatgaaa cagcagggtg
    17881 cagtgattgt tgaccccgca aacctgccga acttcaataa attcggtgcc accgaattgg
    17941 aagtgctgca ttacgaattc aaagccgacc tgaacaagta tcttgctggt tcgaattcca
    18001 aattgaagac gatggctgag atcatcaagt tcaatgaaga acataaatcg cgtgtgctga
    18061 aatactttgg gcaggaacac atgctcgcgg cacaggagaa gggcagcctt cgcgataaaa
    18121 aatatcgaga agcgcttgcc aaaaaccttt tgctcacccg tgaaaatggg atcgatgcca
    18181 cactgaaaaa atacaaactc gatgccatcg tggtcccatc gggcggccct gcatggatga
    18241 tcgacctcgc caacggcgac gccatcaact gggacatgga aagcacatct cctgcggcgg
    18301 tggcgggata tccgcacatc actgtgccag cgggctatgt ctttgggctg cctgtgggta
    18361 tctcattttt ctcaaccgcc tggcaggaac cgacccttat taaacttgcc tatgccttcg
    18421 agcaatccac acaggtgcgc aaacctccgc aattccctgc cacggtgttt ggtaatttgt
    18481 aattggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctt tcttctttca
    18601 tctttcctct caaaaggcct tcctctcccc atgcatctct tcgaactctt cgaaaaaccc
    18661 gtcggctttt tcctcgttgt catggcgatc attctgatca cgccgctgct ttcagaaaaa
    18721 ttgaaattgc ctggcatcat cggcatcatc atcggcggta tgctcattgg tccgcacgga
    18781 tttgggctgc tggaagataa cgaccgcatc cagtttcttt ccaccatcgg tcttgtgtat
    18841 ctcatgttca gcgcaggcct tgaagtggat atcaaccagt tcatgaaagt gcggacgcgc
    18901 gcccttgtct ttggcgtgat cacgtttgcg ttcccccaac ttatgggcat ggggctgggc
    18961 tacatccttg gccttgactg gcttggcatg atcctgcttg gttctgcgtt tgcctcgcac
    19021 actttgatcg cctttcccat cctcaccaaa cttggtgtgg cgcgcaacga agccgtggct
    19081 gtcaccactg gcgcaaccgt cctgaccgat atcggcgcat tcattgtcct tgcagtggtg
    19141 ctgggagcaa aatcaggcgg cggcattacg tttgggtatt ttgcccaact gcttgttttg
    19201 ctaaccattt tcactgccgc gatcatcttt ggactgccgc gcctcggtaa actcattttc
    19261 caaaaactga caggccgcgc tgttgaattt caattcgtca tcgtggcgtt atttgtcgcc
    19321 gcatttaccg ccgaactcat tggcgtgcat gaagtggtcg gcgcatttct tgcggggctg
    19381 gcgatcaact ccatgctgcc gcgtcacagc cctgtggcag ggcatgtgct tttcgtcggt
    19441 gaatcgtttt tcattccgat ttttttgctt tacagcggcc tcatcaccga cccgttgacc
    19501 ttccttaaaa gcccgcagac cattgtcgtt gccatcggcg tcaccatcgt tgcgtatgtt
    19561 tccaaactcg tcgccgcatg gatcaccgcc agaatttaca aatacaccaa atctgaattt
    19621 tggaccgtct acggcctttc gcatgcacag gctgcagtca ccatcccgac tctggtcatc
    19681 ggtttggaaa ctggactttt tgactcaact ctgttcaatg ccgccatttt gatgattcta
    19741 ctcacctcga tcacctcacc cttgatcgtc caacgcttcg cttctgacct gcaaactgct
    19801 tctgccgacg atgagcagac cccgctcttc ggcaggatcc tcgtccctgt ttcagatgtg
    19861 aaacagtcag aagggttggt ttcgctggcc agcctgttgg cgcgcacttc caaaggtaaa
    19921 gtgttggcgg tgagcgtttc caatgacaaa gggttgagca acggcacgac cagttttggc
    19981 attcacaaag aaatgcttgg gcgtgtcacc gaaaccttga atgaccctga aaccgagatc
    20041 gaactcattc cgcgtatggc ttcaacccac gcgcagggaa ttttgcacac ctcgcatgaa
    20101 gagaacgctt cgctcatcgt catggggtgg cgcggcaggc gcaccctgcg tgaaaatgtg
    20161 ctcggctctg tgttggatga agtcatctgg ggttcagata cacccgtcat ggtcggtaaa
    20221 ctgcccctgc cgttgaacag catgacgcgc gtcatttttg tggtgcctgc ggcttccgtt
    20281 ccgccgatcg ctttgcgccg catgttgcaa gccacggttg tttttgccaa atcattgaac
    20341 gtccccctgc ttgtccgtgc tgattcaagt tacctgcaaa accttgaagc cttgctcgtc
    20401 gttaccagag aggatcatcc cgttacgctt gaggttctca aggatcaact caaacctgaa
    20461 aaattggcac acgaagcggt cagcgatttt attattctgc cagggttcgg ttcacgcaaa
    20521 cgtgtggccg atactttggg caatatcccc gaacagatcg ccaaatcttt tgagggcaat
    20581 ctggcgattc tgcattttga taaatagaag atggaaaatg gaccgtaaac tgtggtttac
    20641 ggtccatttt cataaatcaa acgtcgttcc gattccctgc gcaagtgctt tttgatagac
    20701 caaatttgcg gtgaccagat cttccatggc gtggcccatg gatttgtaga tggtcgtttc
    20761 ggtttcgttc ctgcggccag gtttttgacc gagcaggtat tctcccattt ccgtcccgaa
    20821 ctctgcattc agtccctgca attcggcgct gccaacgggc ggcgcttgaa atgcgacttt
    20881 tgattccaca aagacatggc ttgcttccac gaggtcacgt ggtaattctc cgccaggcgg
    20941 gcggtaaccg accgatgaaa cgtggacgcc ttttttgagc cattcggcct ggataactgg
    21001 ttccagcgaa gaggtacaca aacagacaat gtctgcgttg gaaactgctt ctcggacgga
    21061 atccactgcc agtgcgtttg aagcgcgggc ggccaatgcc tgggctttgg aaatgtcctt
    21121 cgaagcgatc cagatctttt cgatgccgct caaagttccg atcatttgta aatgggcctg
    21181 ccccaacact cccgcgccga caatggcaat ggatttgctg tctttgcgcg ccagatgttt
    21241 gatggagagg attgcgcctg cggcggtgcg catggcggtg atatgttcgc catccatgaa
    21301 ggcgatgggt gtgcctgtgt ttgagtcgaa gagtgtaatg gtggcttgat gcgcggggat
    21361 gttttcgtga aagatggtga cgagcttgac cgacatgaat tggttcggca tgtaggcggg
    21421 catgccaacc agcaggcctt tcggcgcgat ggcctgattg cgcggcggca cattgagttg
    21481 acctgcactg agtgccttga agccttcttc cagcacggtc agaagctcgt cggggtcaag
    21541 caggtgttcg atttctttgc gggtgagaag cagcatggat acctcgcgcg atgcagttgt
    21601 gattacaaac ttccacgggt cactttggca gtggcggcag agcgggtgtg gacttccagt
    21661 ttttcaaaaa cggctttcaa atgccgcttg accgtgttgg tggtgatgac aagttgatct
    21721 gcgatctctt tgtttgtcat tcccttggca agcaggtcaa gaacttcgcg ctcgcgttcg
    21781 gtcagggtgc ttagtttgtc gtctgggacg ggagagtctt ttgcttctgt gatgatgcgc
    21841 ttgaacgtgg cacggtcaaa ggcatgtttt tcgataaatg ccacaatgga atatctggaa
    21901 tagatgcgtt gaatctcttc gggttctgaa agcccgctga tgaccactgt ggggattttc
    21961 ctttcctgtg ccagccccag gagtggataa ccattttgcg aaacggcttc attgagcgaa
    22021 aggtcaatga ttgccagtgt gagctggttt tttttcagtg cgcccactgc ttcgccaaaa
    22081 ctggcgcata aaacaggttg aaagccgagg tcgctcaaca gatcttcgag aatactgcgc
    22141 cagcctgcat catcgtccac aattagggct ttgggcatgg cgggcgggtt ttgcagggtt
    22201 ggttgtgcag gtgcaggcgc agctttttcc tcttcaggtt ttggcgcgct ggtcaggatg
    22261 ttgtgaatga tctccttgaa ctggccgcga ttgaagcttt ccttttgcag gaaggtgtgg
    22321 gcatggtggc gggtcaatgc agcaacggcc acttccacgg tggcgaaacc tgtcagcaga
    22381 attgacttgc actcagggtc aagatgtttg atattttcaa ggacgcgcaa tccatccgta
    22441 ttgttgtgat tggctgaaag tgaaaggtcg gctacggcaa ggcggtgcgg atgtgctttg
    22501 agcagcggta tggcttcttc aaggctgcct gccacatcca catccaggcc gcaatcggag
    22561 agaatctcgc tcatgatctg ctgccaggcc tgatcgtctt caaccacaat ggcgcgggga
    22621 aggggcgttg tcatgcacgc acctccgcga tgggcagttt caatacaaag gtggttccat
    22681 gctcgccatc gctttccact tgcacagacc ctccaagccg tgtcatcaat gtgcgcaccc
    22741 accaaagccc gaatccaagt ttgcctgggt gtgaacggct cgaatattcc aattcgaaga
    22801 tctgctcctg caattctggc ttgatccctg gtcccgtatc cgcaacgcgg acctcgacat
    22861 ttttttcgat caccattccg ctgaccgaga tcgtcccatt gcctttcatc gcgtccatgg
    22921 cattctcgat cagattccga aacacgaaag tcaggctttg ggcattggca tttatggtgg
    22981 gcaatgcatc aaggccgctg atgatgatgc gcgtacctgg ctgcacttga atggtgtgga
    23041 tcgcatcctg cacgcgcggc gcaacctgaa tctgttcaat gcggatcggc cgcaggtgtg
    23101 aaaggttttc acgcacgatt tgcatcgccg ccatcgcact gcgctcgatt tcgatcaaac
    23161 tcttgttgag gtaggcgtca ctctcaagca gggtgtcgta tttatcctga atggcctgca
    23221 cacgcacagg gatcgtcccc actttgttgt tcatgttgtg caaaagattc gcggagatat
    23281 ctcccaccgc ggcgaatgtt tctgcgatca gatgctgttc ctgcgacgat cgcaaagctt
    23341 gctggcgcga ctcattctgg atggcgagca cggcatagtt ggcaagacag gccagcactt
    23401 tcttatccca ttcagtggta tcgcttcgga ctccgcccgg ggctgcttga aacaccccaa
    23461 atattcccgc cgctgagtca ttttccccgc tcaagagcgg catgagcaac gcattgggca
    23521 aatccattcc ctgtggatct tgcgcgggaa aatccgcacc gttggaggcg gctagttcca
    23581 tttcgccctg gccattggcc agccaaaccg cactcgacga ggtgttgagc aggtcatttg
    23641 ccattccgca aagtgtgttc aacacctcct ggctggattg tgaaataagc agttgcgccg
    23701 cttcctgcaa tgcatcaagc aggcgcacct cttgaattgc ggtgacagca taagttgcaa
    23761 gcgattgcag catgtggctg tcatcttcag aaaacccgcc gatgcgcgga ctttcgaggt
    23821 ttagaacccc ttcaaggcgt ccgctggcat tgatgagcgg aatgcacaac tcggagcgca
    23881 tttccagttt tgaatcgagc gggtagtaca cggaattcca aggcttttca cgcaaatcgc
    23941 ggatgagcag ggattgtcgg gtacgggcta cgtacgccat cacgctctga cctttgatgg
    24001 gcagtttttc aaccagcggt ttttccaaat gcaccgaact gaccgcacgt gtcacaagat
    24061 cctcgccgct tttatccacc agacggaaga tgccgtactg ggcattggtc aattccagtg
    24121 caagttccag gatggattcg agagtctcct caagccgcat tcgcgaagag atgagcatcc
    24181 ctgcgtggcg cagacggttg agttcatttt ctttttgtgc gaggttgtgt ttaattcccg
    24241 ccacatgcct ggcgtggtaa atcgccatgg ctgcctgatt gacgaaatta tgcagcatca
    24301 attcttcgaa ttgggatagc tggcgttcct cgtacaggta aatgtaaagt gcgccaagca
    24361 cctctttgga agccagcagc ggaaaacaag ccacaacctt caccccaagt ttttcctgat
    24421 aggggtggat ctgcaggtta gcctcttcat aggaaagggt gcggcgtttg cgttggatgg
    24481 cacgcattcc aaagccattt gagcgcggca catcatcgtt tgggttgatg tggcggtact
    24541 cttcgtagcc tttggcatat gaaacgacac gagattcgat ctcaagcctg tcggtgtttg
    24601 gattgtaaat ataaatggag gctgaaaacc caggcaccac acgaatggcg ctatcaacga
    24661 tcaattgcag gctggaagat tggtcttcgc tggaagtgat ctgattgatg gccgtgctga
    24721 tctgattcag actcgccaat gcttcgagag tccgtgggtt gagttcttcg gaagggggga
    24781 tgggtgaatt tgccatggag cgcagatctt tacaggtgtg attttacttc atggctgcaa
    24841 ctcggttgag acctgaatgc atggttagat gaaattttgc atggtctgta ttccaagctg
    24901 gaaggcaaag tagaacaaca caagtccgca ggtggcgttg agaaatcgga agaagcctac
    24961 ggtcatccac tgcctgcccc aggcgatcag cccggcgagg atgaagcacc acaaaacggc
    25021 gccggaaaga aaccctgcaa agaaagtgac aaaatggatt ggttctggtc cgcctgcgat
    25081 ggaggcgaaa acggtggtgc cgaggcctgt ccagaagacg atgttcatgg ggttgccaag
    25141 cgagagaaag gcgccactgg caaaatctcc gcgtgaagaa gcaggttcag agatgtttaa
    25201 tccatttccg tgacgggcat ctttgagcgc atcccaggcg agtttgagca tgagggcaat
    25261 cccgatgagg ctgaggatcg ttcgcgcaat attgttttga atgacgaagg ccagcccggt
    25321 gagtgcaatc accgcccagg tggtatcgcc gatcaaagaa ccgaactgca cgtataaagc
    25381 gggaaagaat ccacgggctg agccgcggcg gaaagtctct gcggtgacga caccaggcgg
    25441 ggcgcagaag gaaatggcga gtatgaaaga agagatgaac aatgaaatat gcatggtgtt
    25501 tctttggtag aaaaatgccg acagtcagat gtgtgtcagg ggcacaccca actgtcggcg
    25561 gggagtgaag cgattttagt cgggctgatt aggcgtcgaa gtagaggacg tactcgtgcg
    25621 gagtcgggcg caggcgaatg gcatccactt cgtgagtgcg tttgtacttg atgtactctt
    25681 cgagcaggtc gggggtgaac acgccgccct tgaggaggaa ttcgtgatcg gcttcgagtg
    25741 cgtggagcac ggcatcgagg ctggaaggca cctgcttgat gagcgccttt tctgctgcgg
    25801 gaagttcgta cagatccaca tccttgggtt cgccggggtc gatcttgttc aggataccgt
    25861 caatacctgc cataagaaca gcggagaaag caaggtaggg gttggcggtg gcatcgggtg
    25921 cgcggaattc aacgcgcttg gccttccaac tcttggtggc agggatgcgg cagatggcag
    25981 agcggttgcg ctgtgagtag gcgatgttta cgggtgcttc gtagccgggc acgaggcggc
    26041 ggtacgagtt ggtggtgggg gatgtcagcg cgaggagggc gggagcgtgc ttgagcaagc
    26101 cgccgatgta gtatttggcg gtctgagaga gacctgcata ccccttctca tcgaagaaaa
    26161 cgttctttcc atccttccac atcgaagagt ggacatgcat accggagcca ttgtcgccga
    26221 agatgggttt gggcatgaag gtcacggtct tgccgtattt gcgtgccacg ttcttgatga
    26281 tgtacttgta aagcaaaaga tcatcagcca tgcgggtgag cgtggtgaag gtcatactca
    26341 tttcgctctg accggcggtg gcaacttcat ggtggtgtag gtccatctga atgccagccg
    26401 cttgcattgc cagcatgaat ttgttgcgca gttcctgcag ggtgtcggtt ggtgaaactg
    26461 ggaagtagcc gcgcttgggc tgaatctggc cgcccttgtt gccttcacgg ttgctgctcc
    26521 accagccttc ttccgagtca atggagaagg aagcttcatg cggggtggat acatagcgga
    26581 ttccgtcgaa gacgaagaat tctgcttcag gcgcgaagta gaccgtatcg gcgaggccag
    26641 tgcttttggc atatgcctca gctttttgcg caacatagcg cggatcgcgt gaataggctt
    26701 cttctgtgat cgggtcaaaa acgttgcagg acagaacgag ggtgggtgtt tctgcggtcg
    26761 ggtctaagaa agcggtatca ggatctgctt tgagaagcat gtctgattcg tgaatttcct
    26821 gaaagccgcg gatggatgag ccgtcgaaag gaatgccttc cttgaagaag tcttctgtga
    26881 ggcggtggac cggcatggtg aaatgctgcc aggttccggg taggtcggtg aagcgcaggt
    26941 caacgacttg cgcgcttttt gccatttcca aaacttcctt gattgacttt gccatggttg
    27001 attttctcct tgcactgatt tattttgact gcgcgattat aggggggatg gcacgggtaa
    27061 gtctattacc caaacgggta cttaatggga aagtggacgg ggaagcggcc cgtccacttt
    27121 tg
//
LOCUS       DAOQLC010000058        27106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_589_length_27106_cov_22.856235, whole genome shotgun sequence.
ACCESSION   DAOQLC010000058 DAOQLC010000000
VERSION     DAOQLC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_27106_cov_22.856235"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /gene="aspS"
                     /locus_tag="PL000_11565"
     CDS             complement(<1..1735)
                     /gene="aspS"
                     /locus_tag="PL000_11565"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNC89583.1"
                     /translation="MYRSNLCGELRASHAGQTVTLSGWVNRRRDHGGVAFFDLRDRAG
                     IVQVTINPDLPKEVLDQVASVRNEWVLQIEGVVQKRPDGMANPKMETGEVEVIAKNVT
                     VLNASKTPPFMVNTDADLPDENMRLKYRYIDLRRERLTKNMVLRHKVIKFMRDYLDQQ
                     GFIEIETPIMFKATPEGARDYLVPSRIYPGQFYALPQSPQQLKQLLMVAGMDKYFQIA
                     RCFRDEDLRGDRQPEFTQLDLEMSYVHRDDVLDLVEGLFTAMIPAVAPHKKLFSSPWP
                     KFSYKEVLERFGSDKPDLRFGMELIDVTDVLAKSEFKVFQSALEAGGVIKCIVAPKSA
                     EMSRKELDALTEVAKSFGAKGMPYLAVTAEGVKGSAAKFFKEEELAALKEKSGAGVGD
                     LIVFASDARAVVNKTLGGLRLWFRDKLDLADKSMMAFAWVVDFPFFAFNEETQAWESE
                     HHPFTMPKMEDLPKFDTNPGEVYSEAYDMVCNGYETASGSIRIHRRDIQMKMFQMLGL
                     SDEDIQTKFGHMLEAFEYGAPPHGGMAPGIDRLVMLLADEPNIREVIAFPKNQNGRDV
                     MADAPSEVEPKQ"
     gene            complement(1991..2902)
                     /locus_tag="PL000_11570"
     CDS             complement(1991..2902)
                     /locus_tag="PL000_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89584.1"
                     /translation="MTNFTIRKGKQTDQFAVFCVFRLSLYDLLQKMKVVTSIPTLAEI
                     ENLYEYYRSFLAHIYDSCDQFWVAEADGQVVGYARSIVRDGVRQLTEFFVMPDHQGLG
                     AGSQLLAKAFPKEGTQNRVICASPDTRALIRYLKTGVRSRTTIYEWSRIPEAVPFETD
                     LVIKPIENTPANIAILNEIDHTILGYTRETDHHWLIQNKRGHFYLRQNQVVGYSYFAN
                     RAGPIAMLNNADFSAALAHIETEMTATVDEVYNEIILCVPMSNPAAVDYVIRRGYHTN
                     AFFEHFLTDKPLGHYENYIFIDPILTT"
     gene            3070..3516
                     /locus_tag="PL000_11575"
     CDS             3070..3516
                     /locus_tag="PL000_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89585.1"
                     /translation="MNYRLMFIINAIASALLGAVLLLMPEFVLVQLGAETYVSTQLMV
                     RFFGGTLLVSGVLLWFLKDVAAKVQKGAGFALLGGSVGGFALSIIGMTSAGIFRSNGW
                     VLLVIFGVFSLVYGYLLFLQPKQSDAKARAPRKPKEAPSAGGGQSV"
     gene            complement(3579..4355)
                     /locus_tag="PL000_11580"
     CDS             complement(3579..4355)
                     /locus_tag="PL000_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89586.1"
                     /translation="MHTPTSATHQFKYQPEKNRRGIMYQYTKSNVDGSAPARVYIRMA
                     DEYVIDVWKFESHNFDAAHVQARMNWDFFSAEQLDSWVVTSDGASRPQARMNVSFEEA
                     VVTVQIQHMEGKTEIGHFPFHIYNFDFISLNMSLPHWVNPESELSIGVVQPNFNPEIR
                     SLLNYEGIATLKFIGNEKRNGTPCRKYSLEGQWLNGQAGQLWVSQTEGHIEDMEIPIP
                     DNPDWDDFKFNLVSTQSMDDAQWEQFISTEIKKLTPVDGE"
     gene            4451..5377
                     /locus_tag="PL000_11585"
     CDS             4451..5377
                     /locus_tag="PL000_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89587.1"
                     /translation="MKSPRFSTLTLYFLSLALFVTACVGGEATATVLPVATEFIPPLD
                     EPTPIPFTGHTPFPTPSSLPAVNQASTSPEWVADFSDPILAKLVNRKPDFQDDFKLVC
                     IYNSQWLDNCPTEDEMPNFIDDLSVLNRGWFYRIPGSTSGPFYSDLKYETLFLNSPKG
                     KSDRDLMVYNPYLLHENFVLSFDFQFKETQPDDFVRFQFNQSKDQSVNLDLWKNKSWS
                     FYGPLQEVSGAFDYFPPELINITIIMLGRECAAYLNDVPFAYLNDCRSEAVVGSFPWA
                     VSFHVIAAPGHVARAAIDNVKLWDLDKIKELQ"
     gene            5507..5848
                     /locus_tag="PL000_11590"
     CDS             5507..5848
                     /locus_tag="PL000_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC89588.1"
                     /translation="MKLQSSDLENGIRLIKLVGKLDSNGTYAIEIEFIKSCAGEKPRI
                     LVDLSKVNYISSIGIPMLVNTARSVMARGGKLGLLNPQRNVLDVLELVGVSMMLPIYY
                     DLKTATAGMLA"
     gene            5876..6409
                     /locus_tag="PL000_11595"
     CDS             5876..6409
                     /locus_tag="PL000_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89589.1"
                     /translation="MFKNLFGNQQSEPQMVMSEGYKNWRGMIFLVTPGQIELNIKEPN
                     KVFGVILDVGVKDMNSNSAFVFTTAVFANGDATFFPSPGGGVIGLGNNPQVAATSKSI
                     VTMGQIFLARAQPAKENSTASVSRVKFYFLTTSGLFVTEDSLKAFQSGPYGQLLGKFG
                     QIRAVAEQMIDKRLGKK"
     gene            6518..6865
                     /locus_tag="PL000_11600"
     CDS             6518..6865
                     /locus_tag="PL000_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC89590.1"
                     /translation="MEIHYRDLKDSMGLVHLIGSLDISGVGQIETRFAGYCAGEQPRV
                     IVDLSEVDFMSSIGIRLLTLNAKSVATRGGKMVLLNPTPDVLSVLEITGIPAIIPIYS
                     HIESAETVLRSSR"
     gene            complement(6922..7386)
                     /locus_tag="PL000_11605"
     CDS             complement(6922..7386)
                     /locus_tag="PL000_11605"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNC89591.1"
                     /translation="MNDARTSTVSSDEKLMGAAAHFFGPLAALIIWGTQKDKSPFIKF
                     HTLQALAFDLVTITSLGIFFFCAFGVVFVGMFGSIFSMTYTSSPESEFRFLLPAVLFP
                     VMITTCITPISLLITAVRLAASFSILSGRDFRYPLIGKWLENFLKNDINSSP"
     gene            complement(7383..8324)
                     /locus_tag="PL000_11610"
     CDS             complement(7383..8324)
                     /locus_tag="PL000_11610"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HNC89592.1"
                     /translation="MENPTSDEKILAALSHAAVIFPMLGPVVPAGIWAFQRQKSKYVR
                     FHAMQAMGYQTYGLWLWFVGMFLSIFLLMIAVIPLEIALSDNPDTLAMSPLIIQPVFF
                     LIILGSWGLFFLGGAIGAVFCLLNREFRYPIIGNWLKQKLFHDQVTDAEFEKWEENWI
                     SGVCHATVILRFWGIITPFMLWILEREHSVKIRFQALQAALYQLLYVVAAIASTAIGV
                     VFYVVFIIVMAIGLNGTSPAANDEIPAWLGAVLIGVMVILILYAIATFIIMPVYYLMA
                     LIGSFRVASGHEFYYPILGDIIAKRMKPMQVEVEPQP"
     gene            8433..9824
                     /locus_tag="PL000_11615"
     CDS             8433..9824
                     /locus_tag="PL000_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89593.1"
                     /translation="MIYLRKFHISEEDGTMANVAPNSVSTRRERVAWYLYDFGNSAYA
                     SVVLLAVYSAYFQKQVVGGAEGSRIWGLTISIAMIIVAIAAPIMGAIADYSGAKKRFL
                     FFFTALSVVFTAMLFFTQKGTVMLAIVCFLLAEIGYRAAQVFYDALLPEIASPGEMGR
                     VAGTGWAIGSAGGIAILLLILPPILMTKSDLLVVRGSLIATAVFFAAAAAPIFLRLTE
                     RAKPQQLPPGENYFSVAFKQLGETIKAARGFNEFLKFMLAYLVYNEGVIIALDFAAIL
                     GAVLFGLEQTGLIIFFILVQATNVLGALLFGNLQDRFGGKPSLSISIMLMVACIAALY
                     FAQNQTHFFIIGGFVGVAMAGVQSVSRAMVATFSPPGKSAEFFGFFALTGRTSSFIGP
                     AVFGWLAAELTLWYQAQGQDLALAEQSGHRLAILSIAAFLIAGWALMLMVNEKKALET
                     VGQMTVEDTTASK"
     gene            9825..10643
                     /locus_tag="PL000_11620"
     CDS             9825..10643
                     /locus_tag="PL000_11620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC89594.1"
                     /translation="MTSKTAIITGAASGIGKHWADVLLKQGGYQLVLADVNEQGLRGS
                     FSESASLRLHALDIRSKEQWQRVLDDALQRFGRIDYLFNIAGISRQSFFLSQPIENID
                     LVLDINLKGPLIGMRLVGEQMMKQGAGHIINVGSLAGLSPTPGNGLYSAAKAGLRNAS
                     IAAAIEWRTKGIFVTVIAPDLVDTPIMQRHLESAKEEVALTYTGVTLTVHDIEKAFWK
                     AMREKPLEINLPRWRGWLTKLNHANPAMMFWLYEPMKRRGMKRFENIRKQRLGK"
     gene            10640..11620
                     /locus_tag="PL000_11625"
     CDS             10640..11620
                     /locus_tag="PL000_11625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC89595.1"
                     /translation="MNQTRYQKVFITGALGFVGRAIAAHYRTLGAQTCGLDVRADVGL
                     NVVAGDVSRPEEWRNAMQGCDLVIHTAAIVTNNVTRAEAWRVNVLGTRCVLDAARQAG
                     VKRFVHVSSLAAMRFNEGDHAADESAPVMPTGNPYVDTKIASEHVVLAAHAKGEIPCT
                     VIRPADVYGPNSRPWTLIPVQMIQKGLFLLPANGQGIFRAIFIDDLVNGIVLAAEKEE
                     AIGQIFILGGEVATTCETFFGYYYRMLGKGKPRTMSTSTAVAIAEAGRMIFKLLGKPT
                     ELGRGAMEMLSKKSTVSNQKAHDVLGWYPQVDLEEGMRRTEVWLREQKIL"
     gene            11725..>12745
                     /locus_tag="PL000_11630"
     CDS             11725..>12745
                     /locus_tag="PL000_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNC89596.1"
                     /translation="MTEITVGELLAKCLQAEGIEMMFGIIDGAHIPFTVHAPKYGIRH
                     INCRHEEGAVHLAEGYTRTNGKPSVVIGSPGPGGANMLAGVSSAYAEGHPIIAIACTR
                     RRLTTDPERGGAWQATDLVEMAKPITKYSALVRQPERLPEMMRAAFRAALTGRPGPVF
                     IAIPDELLGAKIDSDKVPVYPSSKYRMTDMGAGDPAWIEEAAELLANSKKPFLHAGKG
                     VLWADAAAEFLELGNYLAAGMGSSMGARGAVPEDHPHYFFLFDMQATTLARNEADVVL
                     VVGSRLGEYDGWGSHPAWGMPEKQKTIQIDVDANVIGLNRPVDVGILADAKSALKAIL
                     SKVKEK"
     assembly_gap    12746..12845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12846..13592
                     /locus_tag="PL000_11635"
     CDS             <12846..13592
                     /locus_tag="PL000_11635"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC89597.1"
                     /translation="LADAKSALKAILSKVKEKTAARSEMPDLARYRELSQQTMANGFQ
                     FLMAQPTRGLNPGQMVFNARSFFPRNAVTVLDGGNTTLWGVAFNQIYEPRSFLYSVKM
                     GYLGTGIPFAIGAKLAAPGRPVYCITGDGAAGFNIMEMETAVRENAQIIVLVAVDDGW
                     GMERSAHNFGGVPAELQQGVNISTEMRYDLIAQGLGCYAEKVDSVDVLPAALQRAVDS
                     GKPALLHVTVDSAINADPPGYKQFRYVRTL"
     gene            complement(13692..13835)
                     /locus_tag="PL000_11640"
     CDS             complement(13692..13835)
                     /locus_tag="PL000_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89598.1"
                     /translation="MSETTTKIKAKRPSKGMAKHNRLVKQEARKNSVPGTTVKKGKRP
                     EVK"
     gene            complement(14109..14720)
                     /locus_tag="PL000_11645"
     CDS             complement(14109..14720)
                     /locus_tag="PL000_11645"
                     /inference="COORDINATES: protein motif:HMM:NF022307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HNC89599.1"
                     /translation="MERKTQTGYLVLADISGYTSFVAKTEIEHAEKAISILLGNIIER
                     LDDLLSIVKLEGDAVFAYVSDEQVPDCNIMLELLDKTYEIFRDSARSLHAHATCPCKA
                     CRAIPELDLKFLVHHGEYIVQQVAGIKDLMGNDVTLIHRLAKNSVSESTGWNGYVLFT
                     GNCLERMQADKRPFIQQAETYEHLGNVETYVMNMHVRYDEMKK"
     gene            14949..15365
                     /locus_tag="PL000_11650"
     CDS             14949..15365
                     /locus_tag="PL000_11650"
                     /inference="COORDINATES: protein motif:HMM:NF016001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HNC89600.1"
                     /translation="MGEVNDYNQRIINEFRANEGKVGGPFEGKTLLLLHTIGAKSQKE
                     RINPVACISDGERLVVIASKSGAPTHPDWYHNVVANPLVTVEVGMETFQAKASVAEEP
                     ERSRLYDKMVEVMPGFDAYRRKASRVIPVIVLERMD"
     gene            15490..16602
                     /locus_tag="PL000_11655"
     CDS             15490..16602
                     /locus_tag="PL000_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNC89601.1"
                     /translation="MNINPNATMRFPLRFARGLNALIFSMLLLVQSACANNLTAAPTT
                     HPKEGTPVIDTPTPKPSKTPSPSPTTTSTPSIIGAWSDAPAMAIPRSAHAVANSDSAI
                     YALAGTDDHGKPVFEVEVFDGKDWKTETTLPGEGLNAPTASVVDQKLYVVGGFLAVSS
                     SPTNEVQVYDLQTQQWSNASPLPNPRGGHVAVVLDGRIHVFGGGNSVSTLADHSEYDP
                     ATDSWRDLAPLPRSEGSPAAVAIDGKIYVIGGRSGFSDFGDVYIYDPETDTWSTGPSI
                     DPRGTAGAVYYCGGIYLFGGESQSQRKNLDEAWRLDLERNVWEPVTAMPIARKFARAV
                     VFMNSVFIVGGSVVPASSHSPIGSNSVLKFTQPGCS"
     gene            16634..17794
                     /locus_tag="PL000_11660"
     CDS             16634..17794
                     /locus_tag="PL000_11660"
                     /inference="COORDINATES: protein motif:HMM:NF022307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2652 domain-containing protein"
                     /protein_id="HNC89602.1"
                     /translation="MTQQGFLMISDITGYSRYINESELEHARDSLTALLNLLIEHTQS
                     PLVLSKLEGDAVFSYAPAGGFLQRQSLLDMIETTYASFRKALELMALNTTCTCNACRN
                     LPNLDLKFFVHFGDYMTQKLGTFTELVGNDVNLVHRLAKNHIKEKTGFKAYAAFTQSV
                     MDVLGLVDFQNSLTPHRETFADVGEVQIYVLDMHSVWERLKDRVRIEVKPEDALVVRE
                     YIFPVPLSICWEYMTKPEFRQMVLGVEKVEVGNLKQGRSAVNSVYYCYHGNMETRLLI
                     LDWQPFEGYTTDGVTPIGASFSMTIKLEPIEGGTRVINLWGKARGAWIRRKLNDLLQK
                     LIFVSKNDQGYEKLRQRMQQDLADGTTFVSPPIEVDNAQIAALASQALTSEH"
     gene            complement(17835..>20081)
                     /locus_tag="PL000_11665"
     CDS             complement(17835..>20081)
                     /locus_tag="PL000_11665"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNC89603.1"
                     /translation="FTPRPSQQNILRYNGGRLGIAAVPGAGKTHILSALAAKIIREGD
                     LQDGQEVLIVTLVNSAVDNFDKRIKQFFNNPIESLYKYRVRTLHGLAHDIVREKPARV
                     GLEERFSIIDEREAGFIRRESVNAWLASNSLDDYLDPALDNSKQDWIRRSQLPDLVDS
                     LALAFIRSAKDRLLTPESLRAKLDASFAPLPLAELGYSIYADYQRALAYRGAVDFDDL
                     IRYALTLLESDEEFLERLRYRYPYILEDEAQDSSQTQERILALLSGENWVRVGDPNQA
                     IFETFTTASPELLRKFIRENPSQDMPESGRSQPSILAVANHLIDWVLNAHPDPNVRTA
                     LSLPYIIPVPKNDPQQNPPENPEGIKFISRKYSPEDELEAVAKSVKNFVASIAEIPDE
                     EKPTIAVLVPRNARGVEVVNELRKRGIEPIELISSTTETRAAAGSLNYLLSYLADPQS
                     ARKLSKAYEVWRRDWREQATGNDDSRAGESDPELLKEVTAILRRLVNVENFIAPSQAD
                     EWLAAIGEVDSPQVIEELGLFKVNVQRWLNAAVLPIDQLVLTLAQDVFGKASDLALAY
                     KLALVLRKAADDHADWRLPELASELAVIAKNERRFIGFSDDDSGFDPERHRGKVVVVT
                     MHKAKGLEWDRVYMMSVNNYDFPSNMPNDRFISEKWFVRSGLNLEAEALEQLKALESN
                     SEYDWYEEGAATMRSRLDYVKERLRLLYVGITRAKRDLIVTWNSGRQGDATPSLAMSE
                     LMGWWENK"
     assembly_gap    20082..20181
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20182..20433)
                     /locus_tag="PL000_11670"
     CDS             complement(<20182..20433)
                     /locus_tag="PL000_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89604.1"
                     /translation="MSDPYEFIDMAQKAFDFLVKDFGFQVTVTKSQGTYHSEVVFTTD
                     KLKITVGIWGQSNEAWIIFFPQDKNFPPLHFSSLLNRITG"
     gene            complement(20447..21475)
                     /locus_tag="PL000_11675"
     CDS             complement(20447..21475)
                     /locus_tag="PL000_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89605.1"
                     /translation="MKNKQIPTLIGFFILMYACNFGTPASITPTPDRVETQQPATATA
                     EISPTTAPSPSNGPTLANCPMFPADNYWNAPIDTLPTHSQSEAWIDSIGRTVSFHMDF
                     GSGEYDGGLIGIPFNIVAGSAVPKVGFEFDYADESDPGPYPIPENPNQEAGGDRHILV
                     LDTDTCTLYEVFDASFDGGQWRGGSGAIWDLNSNTLRPDTWTSSDAAGLPILPGLVRY
                     DEIAAGEIKHALRFTIEKTAGYIWPARHLTDDPQDGIPPMGARFRLKADYDISGFPQE
                     MQIILQAMKTYGIILADNGSNWYISGAPDDRWDNDMLHLLDVLTGNDFEAVDTSGMIV
                     DVDSGAAK"
     gene            complement(21568..23637)
                     /locus_tag="PL000_11680"
     CDS             complement(21568..23637)
                     /locus_tag="PL000_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89606.1"
                     /translation="MSFYSLNYTLQDLIGYTLAFFLFPLVIVLPGYVFSSALDLFDFK
                     RRLPIVKFGIGLLVSFAVSPIIFNLTSSLGSMTFSIIVIGGFVLAFLFFLAKENFSLQ
                     IAPTAKSLVWICIGWVVFVAYSLVDIHWGDQLYVSVVSFDQTSRVSVIDAMTRTGVPP
                     INPSYFPGAPTLLTFLYYFWYILASLVDILGGTLVDARAALNASSAWSGLGLMAAIAF
                     YLRVRNVNLGRAVWRSARIGVSLLAVSGLDVLPTIIIMLGVRRIYGTVEGWNLQMQIP
                     TWNSSILWAPHHIAAMVACFTAILLALSAREKPLRRKMTLMTIAGMAFASSFGLSIWV
                     TFLFGVFWAVWVLALFIQKTDRSLVVPMIFCGVVAVLLAGSFILGLLQPQGEGGVTQS
                     TVIFDVRTLYLLEGFVTAWPPLARSLLMLALLPLNYFLELGFFIVAGYFWVGSKNKTE
                     IVSDPFYLVEVLLMSVVLLIGSTLRSTIANNDLGWRVWLPGQFILLIWGVDVIETLFS
                     TKSASDSPKADSVKRKNLALTLLILGVLTSSVDALFLRVTWPLRVGVDEGRRGYSARL
                     AYDFLRDQVPADVIMQNNPLPLADRPSGLYGTHQMVISDRTIYGVSLTTYDQFVHDLA
                     FLFTESFSNWQPIDTACQKYSIDLLIFNDLDPAWSSLATLKTQRPALYENEHYSIFAC
                     GSYTKIR"
     gene            complement(23671..24885)
                     /locus_tag="PL000_11685"
     CDS             complement(23671..24885)
                     /locus_tag="PL000_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89607.1"
                     /translation="MKTRELLWILLLAALTTLGLDLVTNAINTQGNAWDFLYYIALAK
                     DGFHADPLASPFAYRYLTPFIVHGISLLGFSIERGFGIIAYFGAIMQLTGIFFFIKWL
                     GNSTKGAYLAMAVTALSLFNVKFLLFDIYRPDHLAYALILLQTYLAFNRKFLPLLLTT
                     LIASQIREFNIIPMVAYLFMSFRTTERGLFIKQAAISTICLVPAILLPRLLIPVTENY
                     QLVGLSGDALLTTVILPFIPSVDVNFIFSIFAYLLPLLFLADLKAIKAAFASLPENQR
                     GYVLAYTLLVLLLSFFGGTDFNRFATFLFLPQIILIGLITPAISSLQISLMLAVTFIF
                     NRLWMHIPDWDVEKYRDFYGGFSLRLNMSTLNRIMELAAFWALGFGVRKWQGTTQTLE
                     SQRHENTTPQRK"
     gene            complement(24974..>27106)
                     /locus_tag="PL000_11690"
     CDS             complement(24974..>27106)
                     /locus_tag="PL000_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC89608.1"
                     /translation="NLHPVDKSSALLYSRFMASLNLLQRQIIESPLDSRIFVSGRAGT
                     GKTTTGVERMRFLLEQGVPADSILMLTPQRTLQEPYLELLYSPERRAGGEVTSATIGG
                     LAKRMCDLFWPIAAEAAGVKNPERAPVFLTLETAQYYMAFIVRPLLEQGYFQSLTIDR
                     NRMYSQIIDTLNKAAVVGFPYTEIGTRLDAAWVGDPGQRRIYQDVQDCAIRFREYCLE
                     HNLLDFSLQLEIFTDILWPQEQVQNYLKQTYRHLIYDNIEEDAPRAHDIIREWMPDFE
                     SALLIYDNDAGYRRFLGGDAASGYALIETCESQIEFSESFVSSENIIQLSNSLVSAVT
                     NQSTGEEAYPPDTMEFIMAHFYPEMLNEVVGKIKTLIEEGVPPAEIVVLAPYLSDALR
                     FSIADRLEKENIPWRSHRPSRSLRDEPASNALLNLSALAHPHWNVRPTKFDMMQTLMF
                     ALDTDLVRAQLLTEVVYGQRDLRLSPFEEIRPEMQERITYRLGEKYTTLRNWLLEYRE
                     AAPLPLDFFLRKLFGEVISQPEYGFHSNMDAVRVAASLIESIRKFRNAMEPFDQTGFD
                     MGREYLSMLADGVIAAQYLESWRSEDKEAVLVAPAHTFLMMNHPVTIQFWLDPGSVGW
                     VQRLSQPLTHPYVLSRGWDPSPAHFWMDTDEVREETDALAKLVGGLLSRCREKVFLAL
                     ADLSESGMEQRGSLLRAFQKVLSLQGQE"
BASE COUNT         6391 a         6570 c         7305 g         6640 t
ORIGIN      
        1 actgcttcgg ttccacttca gacggcgcat ccgccatcac atcgcgtccg ttctggttct
       61 tcgggaaggc gatcacttcg cggatgttgg gctcgtccgc cagcagcatc acgaggcggt
      121 caatgcccgg agccatgccg ccatgcgggg gagcgccata ctcgaacgcc tctagcatgt
      181 gaccgaactt ggtctgaatg tcctcgtcgc tcaagccgag catctggaac atcttcatct
      241 gaatatcgcg gcgatgaatt cttatcgaac ccgaagcggt ctcgtagcca ttgcagacca
      301 tgtcatacgc ttccgaatac acctcgccgg ggttggtatc gaacttcggc aggtcttcca
      361 tcttcggcat cgtgaacgga tgatgttccg attcccatgc ttgcgtttct tcattgaacg
      421 caaagaatgg gaagtccacc acccatgcaa acgccatcat ggatttatcc gccagatcca
      481 acttatcgcg gaaccacaag cgcagtccgc cgagagtctt gttcacaacg gcacgagcat
      541 cagaagcgaa gacgatcaga tcgccgacac ctgctcccga tttttctttc aacgctgcga
      601 gttcttcttc cttaaagaac tttgccgcgc ttcctttcac gccttcggct gttaccgcca
      661 aatagggcat tcccttcgcc ccaaacgact tcgccacttc cgtgagcgca tcgagttcct
      721 tgcgagacat ctccgcagac tttggcgcaa cgatacattt gatcacacca cccgcctcca
      781 acgcggattg gaagaccttg aactcgctct tcgccaacac atcggtgacg tcgatcaact
      841 ccatgccaaa gcgcaggtcg ggcttatccg agccaaagcg ctcgagcact tccttataag
      901 agaacttggg ccacggcgac gagaataatt tcttgtgcgg agccacggcg ggaatcatcg
      961 cggtgaacaa gccttcaacc agatccagca catcgtcgcg gtggacgtag gacatctcaa
     1021 ggtcgagttg ggtgaactca ggctgacggt cgccgcgcag gtcttcatcg cggaagcagc
     1081 gcgcgatctg gaagtacttg tccatgcctg cgaccatcag caattgcttc aattgctggg
     1141 gtgactgggg cagggcgtag aactgtcccg ggtagatgcg ggaaggcaca agatagtcac
     1201 gcgcgccttc gggggtcgct ttgaacatga tcggggtctc gatctcaatg aagccctgtt
     1261 ggtcaagata atcgcgcatg aacttgatga ccttgtggcg cagcaccata ttcttggtca
     1321 ggcgctcgcg gcgcagatca atgtagcgat acttgaggcg catattctcg tcgggcagat
     1381 ccgcgtcggt gttgaccatg aatgggggag tcttcgaggc gttgagcacg gtcacattct
     1441 tggcaatgac ttccacttcg cccgtttcca ttttgggatt cgccatcccg tcggggcgct
     1501 tttgcaccac gccttcgatc tgcaagaccc actcgttgcg aacgctggca acctgatcga
     1561 gcacttcctt tggaaggtct gggttgatgg tgacctgaac gatgcctgca cggtcacgca
     1621 ggtcaaaaaa tgccaccccg ccatggtcac ggcgtcgatt cacccagccg gacaaagtca
     1681 cggtctgacc cgcatgactg gctcgtaatt ctccacataa attagatcta tacatagtgc
     1741 gcctccgaaa tatttccatt agacctgtgt cttaatggtg tatataaaat aaatcgccct
     1801 tcgcatttgc gtcgggcgat ttggtttcga cttttgttat ttgtcaacca gcaacgcttc
     1861 gccctacgat tcggggatcg tcattgtcat tattattgtt attggaaaat tggatgtggt
     1921 tgaccatcgt gcgcggatta tagcacaaag ttatctttcc aaagcagatt cgcgcttgaa
     1981 attcaaaaaa tcatgtggtc aggatcgggt ctataaaaat atagttttca taatgaccca
     2041 atggcttgtc ggtaaggaag tgttcaaaaa aagcattcgt gtgatagccg cgcctgatga
     2101 cataatccac ggcggcgggg ttgctcatgg gtacgcaaag gatgatctcg ttatatacct
     2161 cgtccacggt tgcggtcatt tcagtttcaa tatgtgccag agcggcggaa aaatctgcat
     2221 tgttcagcat ggcgatggga cccgcccgat ttgcgaaata actgtaccca accacttgat
     2281 tttgccgcag gtagaaatgt ccacgcttgt tttggatgag ccagtgatgg tcggtttcgc
     2341 gggtgtaacc gaggatggtg tggtcaatct cattgagtat ggcaatgttg gcaggtgtgt
     2401 tttcaatggg cttgatgacc agatcggttt caaacggaac cgcttcaggg atacggctcc
     2461 attcataaat ggtggtccgt gagcgcactc ccgttttcag gtagcggatc agggcgcggg
     2521 tatcggggct ggcgcagatg acacggtttt gtgtaccctc tttcgggaag gcttttgcca
     2581 ataactggct gccagctccc agtccctgat gatcgggcat gacaaaaaat tccgtcaatt
     2641 ggcggacgcc atcccgaaca atggatcgcg catacccgac gacttgtcca tccgcttcgg
     2701 ccacccaaaa ctggtcacaa gaatcgtaaa tgtgagccag gaaacttcga tagtattcat
     2761 ataaattttc gatttccgcc agagtgggga tgcttgtcac tactttcatt ttttgaagca
     2821 ggtcatacaa agacaggcga aatacgcaaa aaactgcgaa ttgatcggtt tgtttccctt
     2881 ttcgaattgt gaagttggtc attgcggctc cttgtgtggc tagtaaaaat cgcaaatagg
     2941 aaaaatgtgg gatatggagg tatgggccat gcaaagcaaa tgtaaggttg ggcgggggat
     3001 atttcttgcc tgcccatttg gtgatgctgt atatttattt tatcaacacc actatgaagg
     3061 agagaaaaaa tgaactaccg tttgatgttt atcattaatg cgattgcctc tgctctgttg
     3121 ggcgctgtgt tgctgctcat gcctgagttt gtccttgtac agcttggtgc cgaaacctat
     3181 gtttccaccc agctcatggt caggtttttc ggcggcacct tgttggtttc gggtgtgttg
     3241 ctctggttcc tgaaagatgt tgccgccaag gttcagaaag gtgcagggtt tgccttgttg
     3301 ggcggttcgg tgggcggttt tgctctgagc attatcggca tgacctctgc tggtattttc
     3361 cgctcgaacg gctgggtgtt gctggtcatc ttcggcgtat tctcgctggt gtatggttac
     3421 ctgctcttcc tgcaacccaa acaatctgat gcgaaagccc gcgccccgcg taaacccaag
     3481 gaagccccct ctgccggtgg cggccagtcc gtctaaattt tgttgaaatc aaaaagtcgg
     3541 gaccgtagtc tcgacttttt gatttaatgg ccatgttttt attcgccatc cacaggggtc
     3601 agttttttga tttcggttga aatgaattgc tcccactgag catcatccat gctttgagtg
     3661 gagacaaggt tgaatttgaa atcgtcccaa tcgggattat cgggaattgg aatttccata
     3721 tcttcaatat gaccctcggt ctgactcacc caaagctggc cagcctgccc attcagccac
     3781 tgaccttcaa gggaatattt acggcaggga gtgccattcc gtttttcatt gccgataaac
     3841 ttcaacgtgg cgatgccctc atagtttaaa agagacctga tctcaggatt gaagtttggc
     3901 tgaaccacac cgatacttaa ttcgctctcg gggttcaccc agtgaggcag gctcatgttt
     3961 aagctgatga aatcgaagtt gtaaatatgg aatgggaaat gcccgatttc agttttgcct
     4021 tccatatgct gtatctgcac tgtaacgact gcttcttcaa aagagacgtt catgcgggcc
     4081 tgcggcctgc tggcgccatc tgaggtgacc acccatgaat caagctgttc ggcggagaag
     4141 aaatcccaat tcatgcgcgc ctgtacatgg gcggcatcaa aattatgcga ctcgaatttc
     4201 cagacatcga tgacatattc atctgccata cggatataaa cccgcgcagg ggcagaccca
     4261 tccacgttgg atttggtata ttgatacatg attccgcggc gatttttttc gggttgatat
     4321 ttaaattgat gcgtggctga ggtgggggtg tgcatggaat ctcctgtaat aaagtctttt
     4381 ttattataat ctcctgatac aacggtttcc caaaaatcag catgggatac tataatgaaa
     4441 accatatccc atgaaaagcc cgcgattttc gacattaact ttatattttt tgagccttgc
     4501 cctgtttgtc actgcctgtg ttggcgggga agctactgcc actgttttgc ctgtggccac
     4561 agaattcatc ccacctttgg atgagcccac tcccatcccg tttacggggc ataccccgtt
     4621 tccgactccc tccagcctgc ccgcagttaa tcaggcatcg acttctcctg aatgggtggc
     4681 tgatttttcc gatccgattc ttgcgaagct ggtcaatcgg aagcccgatt ttcaagatga
     4741 tttcaaattg gtctgcattt acaactctca gtggctggat aactgcccca cagaagatga
     4801 gatgcccaac tttattgatg acctttcggt tttgaaccga ggctggtttt acagaatccc
     4861 tggcagtacc agcgggccgt tttattcaga cttaaagtat gagaccctgt ttttgaattc
     4921 acccaaggga aaaagcgaca gggatttgat ggtatataat ccgtacttgc tgcatgaaaa
     4981 tttcgtgctg agtttcgatt ttcaatttaa agagacccaa cccgatgatt ttgtgcgttt
     5041 tcagttcaac cagtcgaaag accaaagtgt gaacctggat ctatggaaga ataagagctg
     5101 gagtttttac ggtccattgc aagaggtttc tggtgcgttc gattattttc caccggagtt
     5161 gatcaatatc acgatcatca tgctgggacg ggaatgtgca gcgtatctca acgatgtgcc
     5221 atttgcttac ctcaatgatt gcaggtcgga ggcagtggtg ggctcgtttc cgtgggctgt
     5281 ttcgttccat gtcattgctg cgccggggca tgtggcacgg gcagcaattg ataatgtgaa
     5341 gttgtgggat ttggataaaa tcaaagaact ccaataaggt atggaagcgg actcgaagcg
     5401 aagtgttttc gggttgggtg tttcaaagat tgggatcaat tttcggttaa gcgcgaagcg
     5461 gtcattttgt cgaaattatt tatatcaatc ataaggcagg ttttatatga agctacaaag
     5521 cagtgacttg gaaaatggca tccgtttgat caagcttgtt gggaagttgg acagtaatgg
     5581 gacctatgcg atcgagatcg agtttatcaa aagctgcgca ggggagaaac cgcggatttt
     5641 ggtggacctt tcgaaggtga actatatttc atccattggg attccgatgc tggtgaatac
     5701 ggcaaggtcg gtgatggcgc gcggtggaaa attgggattg ctcaacccgc agcgcaatgt
     5761 gttggatgtg cttgaacttg tgggggtctc gatgatgctg ccgatctatt atgaccttaa
     5821 aacggcgacg gctgggatgc tggcatagcc gcgaatttcc aaaggagaga ggcgtatgtt
     5881 caagaattta tttggcaatc aacaatctga accgcagatg gtcatgtctg aggggtataa
     5941 gaattggcgt ggcatgattt ttttggttac gccgggccag atcgagttga acattaagga
     6001 gcccaacaag gtgtttgggg tgatcctgga tgtgggggtt aaggacatga attcaaattc
     6061 ggcttttgtt tttacaactg ccgtttttgc aaatggtgat gcaaccttct ttccatctcc
     6121 gggcggggga gtgatcgggt tgggaaacaa cccgcaggtg gctgcgacct cgaaatccat
     6181 tgtgacgatg ggacagatct tcttggccag ggcccagcct gcgaaggaaa attcgacagc
     6241 cagtgtgagc cgtgtgaaat tctattttct aaccacttct ggtttgtttg tgactgaaga
     6301 ttctttgaag gctttccaat ccgggcctta tggccagttg ttgggtaagt ttggccagat
     6361 ccgtgctgtg gcggagcaga tgatcgataa gcgcctcggt aaaaaataac agcttgttca
     6421 tttatagtgc agattctttg aaaccataac ctgttgttaa caaaaaatcc tgttgacctt
     6481 aatataattg cttgaaccac ggtccacagg agaaaacatg gaaattcact atcgtgattt
     6541 gaaagattcg atggggttgg tccacttaat tggcagcctt gatatttctg gggttgggca
     6601 gatcgaaacc cggtttgcag gttattgtgc aggggagcag ccgcgtgtga tcgtggatct
     6661 gtctgaagtg gattttatgt cttccattgg gatacgcttg cttacactga atgccaagtc
     6721 tgtggcaact cgcggcggga aaatggtatt gttgaaccca acccccgatg tgctgtcagt
     6781 gcttgagatt acgggcattc ctgcgatcat cccgatctat tctcacattg agtcggctga
     6841 gacggttttg agatcttcca gataagagcg tttttcaaac cgccccgaag ctttggcctt
     6901 cggggcggtt tcatttcatt ttcaggggga tgaattgatg tcgtttttta agaagttttc
     6961 aagccatttt ccgatcagtg ggtagcgaaa atctcttccg ctcaggatgg agaaggatgc
     7021 ggccagccgc actgcagtga taaggagaga gatgggggtg atgcaagtgg tgatcatgac
     7081 tggaaataga acagcaggga gcaggaaacg aaattcgctt tcgggtgagg aagtgtatgt
     7141 catggaaaat atgctgccga acattcccac gaatacgact ccaaatgcac aaaagaagaa
     7201 aatccccagg cttgtgatgg tgacaaggtc aaatgccaga gcctgcagag tgtgaaattt
     7261 aataaaggga gacttgtctt tttgagtgcc ccagatgatc aatgcagcca gcggcccgaa
     7321 aaagtgggcc gctgcgccca tcagtttttc atcggatgaa actgtggatg tcctggcgtc
     7381 gttcatggct ggggctcaac ttcaacctgc atgggtttca ttctcttggc tatgatatcg
     7441 ccaaggatgg ggtaataaaa ctcgtgtccg ctggcaactc tgaagctccc gatcaatgcc
     7501 attagataat agacaggcat aatgataaag gtggctattg cgtacagaat gaggataacc
     7561 atgacgccga tcaagaccgc cccaagccag gcggggattt catcgtttgc cgccgggctg
     7621 gtgccgttta ggccaatcgc catcacgatg atgaatacta catagaacac aacgcctatg
     7681 gcagtggatg caatggctgc caccacatag agaagttgat ataaagctgc ctgcaatgcc
     7741 tgaaaacgga ttttgaccga gtgttctcgt tccagtatcc aaagcatgaa cggggtgatg
     7801 atgccccaga acctcaagat cacggttgca tggcagacgc cgctgatcca gttctcttcc
     7861 catttttcaa attcggcatc ggtgacctgg tcgtggaata acttctgctt tagccagtta
     7921 ccgatgatgg ggtaacgaaa ctcacggttg agcaagcaaa aaactgcgcc aatggcccca
     7981 ccaaggaaaa agagtcccca cgatccgaga atgatcagaa aaaagacagg ctgaattatt
     8041 aacggactca ttgcaagcgt gtcggggtta tccgacagtg caatttcgag cgggatgacc
     8101 gctatcatga gtaaaaaaat tgacaggaac attcccacaa accacaacca caagccgtag
     8161 gtttggtagc ccatggcctg catggcatga aagcggacat acttcgattt ttgacgttga
     8221 aatgcccaaa taccggcagg cacaacaggg ccgagcatcg ggaaaataac cgctgcatga
     8281 gataacgctg ccaggatttt ttcgtcagaa gttgggtttt ccatttgatt tcctcctatg
     8341 gtgctttgga tgtcgcaatc atactcccat ttgatgaata tattcaatat ttgaagattt
     8401 ttttacccaa ccccacggcg gttcttttcg tggtgatata cttgcgcaaa ttccatatca
     8461 gtgaggagga cgggacgatg gcaaatgtcg caccaaattc tgtatccaca cgtcgtgaac
     8521 gtgtggcatg gtacctgtac gattttggga attccgcata tgcttcggtg gtgttgcttg
     8581 cggtctactc tgcttatttt caaaagcagg tggtgggcgg ggccgaggga tcgcggatct
     8641 gggggttgac catttcgatt gcgatgatca ttgtcgccat cgctgcgccg atcatggggg
     8701 ccattgccga ttattcgggc gcaaagaaac gttttctgtt cttcttcacg gctctttcag
     8761 tggtttttac ggccatgctt ttctttactc aaaaaggcac ggtgatgctg gcgatcgtat
     8821 gctttttgct ggcagagatc ggatatcgtg cagcgcaggt cttttatgat gcgcttctgc
     8881 ccgagatcgc ttctcccgga gagatgggac gtgtggcagg aactggttgg gccattggct
     8941 cggcaggtgg cattgccatt ctgcttttga ttcttcctcc catattaatg accaagagcg
     9001 acctgttggt cgtgcgtggt tctctgatcg ccacagcggt cttttttgca gcggcggctg
     9061 cccctatttt tctgcgcctg accgagcgtg ccaagcccca gcaattgccg cccggtgaaa
     9121 attatttcag tgtggctttc aagcaattgg gtgaaaccat caaggctgcg cgcggattca
     9181 acgaattcct caaattcatg cttgcgtacc tcgtttacaa cgaaggcgtg atcattgcgc
     9241 tcgattttgc ggccattctt ggtgcggtgc tttttggtct ggagcagacc gggttgatca
     9301 tttttttcat ccttgtgcaa gcgaccaatg tactgggtgc gctgctcttt ggaaatttac
     9361 aggaccgttt cggcggcaag ccttcacttt cgatctcgat catgttgatg gtggcctgca
     9421 ttgccgcctt gtactttgca cagaaccaga cccacttctt tatcatcggt ggatttgtgg
     9481 gggtcgcgat ggcgggtgtg caatcggtca gccgtgcaat ggtggctacg tttagtccgc
     9541 ctggaaagag cgctgaattt ttcggttttt tcgcgctcac ggggcggacg tcttccttta
     9601 ttggcccggc agttttcggg tggttggctg cggaattgac gttgtggtat caggctcaag
     9661 ggcaggatct tgcccttgcg gagcaatccg gtcatcgctt ggccatcctc tccattgctg
     9721 catttttgat cgcaggttgg gcgctcatgt tgatggtgaa tgagaagaag gctttggaga
     9781 cggttgggca aatgacggtg gaagatacga ccgcttcaaa ataaatgacc tctaaaactg
     9841 cgatcatcac cggcgccgcg agcggcatcg gcaaacattg ggcagacgtc ctgctgaaac
     9901 agggcggcta ccaacttgtg ctggcggatg tgaatgagca gggattgcgc ggatcgtttt
     9961 ccgaaagcgc gtccctgcgc ttgcatgcgt tggatatccg ctcgaaggag caatggcagc
    10021 gtgtgctgga tgatgcgctg caacgcttcg ggcggattga ttatctcttc aacattgcgg
    10081 gcatcagccg ccaatccttt ttcctttcac aacccatcga aaacattgac ctcgtactcg
    10141 acatcaactt gaaaggtccg ctcattggca tgcggttggt gggcgagcag atgatgaaac
    10201 agggcgcggg tcatatcatc aatgtcggat cgttggcggg gctttccccc acaccgggga
    10261 atggattgta ttcagctgcc aaagcaggat taagaaacgc ctcgatcgcc gccgcaattg
    10321 agtggcggac gaagggcatt ttcgtcactg tgatcgcgcc tgatctggtc gatacgccta
    10381 tcatgcagag acatctcgaa agcgcaaaag aggaagtggc gctgacctac acgggcgtca
    10441 cgctcacggt tcacgatatt gaaaaagcct tttggaaagc catgcgcgag aaaccgctgg
    10501 agatcaacct gccacgctgg cgcggctggc tgacgaagtt gaatcatgcc aacccggcga
    10561 tgatgttctg gttgtatgag ccgatgaaac gtcgcggcat gaagcgtttt gaaaacatcc
    10621 gcaaacagag actcggaaaa tgaatcaaac ccgctatcaa aaagttttca tcacaggtgc
    10681 gttgggcttt gtggggcgtg ccattgccgc gcattaccgc actctcggcg cgcagacctg
    10741 cggactcgac gtccgtgctg acgtggggtt gaatgtcgtt gcaggggatg tctcccgccc
    10801 tgaagaatgg cggaatgcaa tgcaaggctg tgacctggtc attcacaccg ccgccatcgt
    10861 gaccaacaat gtgaccaggg cagaagcatg gcgggtgaat gtgcttggca cgcgctgtgt
    10921 gctggatgcg gcgaggcagg cgggggtgaa gcgttttgtg catgtctcat cattggctgc
    10981 gatgcgattc aatgaaggcg accatgccgc tgacgaatcc gcgcctgtga tgccgacggg
    11041 caacccgtat gtggatacaa aaatcgccag cgagcatgtt gtccttgcgg cccatgccaa
    11101 gggcgaaatt ccatgtactg tgattcgccc tgccgatgtg tatggtccca actcccgccc
    11161 gtggactttg attcctgtgc agatgatcca aaagggattg ttccttctgc ctgccaacgg
    11221 gcagggaatt ttccgcgcga tcttcattga cgaccttgtg aatggcattg tgctcgcagc
    11281 ggaaaaagag gaagccatcg gtcagatctt catcctcggc ggggaagtgg cgaccacttg
    11341 cgaaactttc tttggctatt actaccgcat gttgggcaag ggaaaaccgc gcacgatgag
    11401 cacctccacg gcggtggcga tcgcggaagc ggggcgtatg atcttcaaac tgcttggcaa
    11461 gcccaccgag ttgggacgcg gcgcgatgga aatgctctcg aagaagagca cggtatccaa
    11521 ccaaaaagcg catgacgttt tgggctggta tccgcaagtg gatttggagg aggggatgcg
    11581 caggacggaa gtttggttga gagagcagaa gattttgtaa atgcatttac cacaaaggag
    11641 acaaagggtc acaaaggaaa ccctcaaaag acctttgtgt acctttgtga ccctttgtgg
    11701 ttgaaaaaac ttgaggagga acttatgact gaaataacag ttggtgaatt actcgcaaag
    11761 tgtttgcagg cagagggcat cgaaatgatg ttcggcatca ttgacggggc gcatattccg
    11821 ttcacggtcc atgcgccgaa gtatggcatt cggcacatca attgcaggca tgaagaaggc
    11881 gctgtccatt tggcggaggg atacacacgc acgaacggca agccgagcgt ggtcatcggc
    11941 agcccgggac cgggcggcgc gaatatgctg gcgggcgttt ccagcgccta cgcggaggga
    12001 catcccatca ttgcgattgc ctgtacccgc cgccgactca ccactgaccc ggagcgaggt
    12061 ggcgcgtggc aggcgactga ccttgtggag atggcaaagc ccatcaccaa gtacagcgcg
    12121 ctggtgcgtc aacccgaacg cctgcccgaa atgatgcgtg ccgccttccg tgccgcactg
    12181 acggggagac cgggccctgt tttcattgcc atccccgatg aattgctcgg cgcaaaaatt
    12241 gattcggaca aagtacctgt ctatccgtcg tccaaatatc gcatgaccga catgggcgca
    12301 ggtgaccccg catggatcga agaagccgcc gagttgttgg cgaattccaa gaagcctttt
    12361 ctccatgcgg gtaagggtgt gttgtgggca gatgccgccg cagaattttt ggagttggga
    12421 aattatctcg ccgcgggcat gggctcgagc atgggcgcgc gcggcgctgt ccctgaagac
    12481 cacccgcatt acttcttcct ttttgatatg caggcgacaa ctctggcacg taatgaagcg
    12541 gatgttgtcc ttgtggtcgg ttcgcggctg ggtgaatacg atggctgggg ttctcatcca
    12601 gcgtggggaa tgccagagaa acaaaagaca atccaaatcg atgtcgatgc gaatgtcatc
    12661 gggttgaatc ggcctgtaga tgtgggcatc ctcgcagatg cgaagtctgc gcttaaggcg
    12721 atcttatcca aagtgaaaga gaagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12841 nnnnnctcgc agatgcgaag tctgcgctta aggcgatctt atccaaagtg aaagagaaga
    12901 ccgccgcccg cagtgaaatg cccgacctcg cgcgttatcg tgagttgagt cagcagacga
    12961 tggcgaacgg ttttcaattt ttgatggcgc agcccacacg cggactcaac cctgggcaga
    13021 tggtgttcaa cgcgcgcagt ttctttccgc gcaacgcggt gaccgtgctc gatggcggca
    13081 atacaacctt gtggggcgtg gcgttcaatc aaatttatga gccgcgcagt tttctgtatt
    13141 cggtgaagat gggctatctt ggcacgggca ttccgtttgc cattggcgcg aaacttgccg
    13201 cgcccgggcg acctgtatat tgcatcacgg gcgatggggc ggcgggcttc aacatcatgg
    13261 agatggaaac tgctgtgcgc gagaatgcgc agatcattgt gctcgtggct gtggatgacg
    13321 gctggggcat ggagcgcagc gcgcacaact tcggcggcgt gcccgcggag ttgcagcagg
    13381 gcgtgaatat ttccacggag atgcgctatg acttgatcgc gcaaggcttg ggatgctacg
    13441 ccgaaaaagt tgattcggtg gatgtgttgc ccgccgcact ccaacgggcg gttgattctg
    13501 gcaaaccggc cttgctccat gtgacggttg actctgccat caatgccgac ccgcccgggt
    13561 ataagcaatt taggtatgtg aggacgttgt aaaagtgaaa cggctttaga caccgtttac
    13621 gatgggaaat gttaaatcaa aaaagtggtc acgtggcttg atgtcacgtg accacttttt
    13681 agtccgcggt tttattttac ttcggggcgt ttgccctttt tgaccgttgt gccgggaaca
    13741 ctgttcttgc gggcttcctg cttaaccaga cggttgtgtt tggccatacc cttcgatggg
    13801 cgcttggctt tgatcttggt ggtggtttca ctcatttgac ttgctccttt tggtgttgcc
    13861 tgatttttgt ttcggaccga atcgccagat acaccttcca tgaggcggct gtggcttgtt
    13921 ccgatgtgat gaggttttcg ttgggctgct tacaagcgat acttcaaatc ccccgcatat
    13981 aggcaattta ccatcaagtg acctgatttc attgtactac ggagtacggg attcgccctg
    14041 cagaaataaa aacggtctcg tgaatatttc accagagacc gtttttgatg ttcccatata
    14101 aagcgggttt attttttcat ttcatcgtag cggacatgca tgttcattac gtatgtttcc
    14161 acattgccaa ggtgctcata ggtttcggct tgctgaatga acggcctctt atccgcctgc
    14221 atacgttcca gacagttgcc tgtaaaaaga acatatccat tccaccccgt ggattcagag
    14281 acgctgttct ttgcgagcct gtggatcagg gtgacatcgt tccccataag gtctttgatc
    14341 cctgccactt gctgaacgat gtattcccca tggtgaacca aaaatttcag atcaagttcc
    14401 gggatggccc gacacgcctt gcatggacag gttgcgtgtg cgtgcaaaga cctggctgag
    14461 tctcggaaaa tctcataggt tttatcgagc agctcaagca tgatattgca gtcgggcacc
    14521 tgctcatctg aaacatatgc aaaaacagcg tcaccttcca gtttgacgat ggaaagcagg
    14581 tcgtccaatc tttcaataat atttcccaat aaaatggaaa tagccttctc tgcatgctcg
    14641 atctcggttt tggcgacaaa ggatgtgtac cctgaaatat cggcgagtac caaatagccc
    14701 gtttgtgttt ttcgttccat caaatctcct gtttttgaaa ctgatttttt tattctacct
    14761 tgaatcggat agatgcactc tgtattttgt gggctagtgc agatggaatc cttgactctc
    14821 catgtccgag gccatttttt tgcaacccgc agttgctgac ctaaacgttt ttccaggtta
    14881 ctgcgatggt aagaacttaa tttttggcga catcgggagc atatattttc aaaggagaat
    14941 tacaaataat gggcgaagtg aacgactata atcaaagaat cattaatgag tttcgagcca
    15001 atgaaggcaa agtgggcggg ccatttgaag gcaaaaccct gcttcttttg cacaccattg
    15061 gggcaaagag tcagaaagag cgcattaacc cggtggcttg tatcagcgac ggagagcggt
    15121 tggttgtgat cgcctccaag agcggggcgc ctacccatcc tgattggtat cacaatgtgg
    15181 ttgccaatcc actggtgacg gttgaggtgg ggatggaaac ctttcaagcc aaagcctcgg
    15241 ttgctgaaga acccgaacgt tcccgcctgt acgacaaaat ggtggaggtc atgcctgggt
    15301 ttgacgccta tcgccgcaaa gccagccgtg tgatccctgt cattgttttg gagcgcatgg
    15361 actagggatt cacaacccga aattttcgtc tgcagcaaaa acggtcttgg aggatgctcc
    15421 gagaccgttt ttcactttcc ccttctccag taggttgtca tgcatttgtg ctgatgatag
    15481 attctgtcca tgaatatcaa cccaaatgcc accatgcgat tccccctgcg ttttgcacgc
    15541 ggattgaatg ccttgatctt ctctatgctt ttgcttgttc agagcgcctg tgcaaacaat
    15601 ctgaccgctg caccaaccac tcatccgaaa gaaggcaccc ctgtgattga tacgcccacc
    15661 ccaaaaccat ccaaaacacc ctcgccttca ccgacaacca cctccacacc ctccattatc
    15721 ggcgcatgga gtgatgcccc tgcgatggcg atcccgcgtt ctgcgcatgc agtcgcaaat
    15781 tccgactcgg cgatttatgc attggctggg accgatgacc acggcaagcc ggtattcgaa
    15841 gttgaagtgt tcgatggcaa ggattggaaa acggaaacga ccctgcccgg tgaaggactg
    15901 aatgccccca cggcttcagt tgtggaccaa aagttatatg tggtcggcgg ctttttagca
    15961 gtgtccagca gccccaccaa tgaagtccag gtgtatgatt tacaaaccca gcaatggagt
    16021 aatgccagtc cgcttccaaa cccgcgcggc ggacatgtgg cagttgtgct tgatggcagg
    16081 atccatgtct ttggcggagg gaattccgtt tccacacttg cggatcattc cgaatatgac
    16141 cccgccacag actcctggcg tgaccttgct ccattgccgc gctcagaagg cagcccggcg
    16201 gcggtcgcca ttgatgggaa aatctacgtc attggcggca gaagcggatt ttccgatttt
    16261 ggcgatgtgt acatttacga ccccgaaaca gacacatggt caacagggcc gtccattgat
    16321 ccgcgcggaa cggcaggagc ggtctattat tgcgggggca tttatttgtt cggcggcgag
    16381 tcacaatcgc agaggaaaaa tctggatgaa gcctggcgtt tggatctgga aagaaatgtt
    16441 tgggaacccg tgaccgcaat gcccattgcg cgaaaatttg cccgtgccgt ggtttttatg
    16501 aattctgttt ttatcgttgg cggcagcgtt gtgcctgcaa gcagtcattc gccgatcgga
    16561 agcaactctg tgctgaagtt tacacagcca ggttgttcat aaatttcaac taacctgtga
    16621 tataaaccat gacatgacgc aacaaggctt tttgatgatc tctgatatta caggctactc
    16681 gcggtatatc aacgagtcgg agcttgaaca cgcgcgcgat agcctgacag ccctgttgaa
    16741 tctattgatc gaacatacac aatctccatt ggtgttatcc aaactggagg gggatgctgt
    16801 tttttcgtat gcgccagcag gcggtttcct gcagagacaa agcctgctgg atatgatcga
    16861 gaccacgtat gcgtcgttcc gcaaagcgtt ggaactgatg gcgctcaaca ccacctgcac
    16921 ttgcaatgcc tgtcgaaacc tgcccaacct tgacttgaag ttcttcgtcc attttggtga
    16981 ttacatgacc cagaagctgg gaacattcac ggagctggtg gggaatgatg tcaatttggt
    17041 gcatcggctg gcaaagaatc acatcaaaga gaaaacaggc ttcaaggcgt acgctgcgtt
    17101 tacccagtcg gtgatggacg tgttgggact tgtagatttc caaaacagcc tgaccccgca
    17161 tcgtgaaact tttgcggatg tgggggaggt gcaaatatac gtgcttgata tgcattcagt
    17221 gtgggaacgc ttgaaggaca gagtcaggat cgaggtcaag cccgaagatg cgttggtcgt
    17281 tcgcgagtat attttccctg tgccgttgtc catctgttgg gaatacatga caaagcccga
    17341 attccgtcaa atggtgcttg gagtagaaaa agtggaagta ggcaacctga aacagggacg
    17401 ttcagcggtc aattcggttt actactgcta tcatggcaac atggaaaccc gcctgcttat
    17461 tttggactgg caaccctttg agggatatac aacagacggc gtgacgccta tcggcgcgag
    17521 ctttagcatg acgatcaagt tggagcccat cgagggcgga acgcgagtga tcaatttgtg
    17581 ggggaaagcg cgtggagcgt ggattcggcg caaactaaac gatctgctcc aaaaattgat
    17641 ctttgtctca aaaaacgatc agggttacga aaaactgcgt caacgcatgc aacaagacct
    17701 tgccgatgga actacatttg tctccccgcc cattgaagtg gataatgctc agattgcggc
    17761 acttgcttct caggcattga cctccgagca ttaattatca catcccgaga ccttttttca
    17821 tccttcagca ttcatcattt gttttcccac catcccatca actcactcat cgccagactc
    17881 ggcgttgcat cgccctgacg gcctgaattc cacgtcacga tcaaatccct ttttgcgcgg
    17941 gtgatgccta cgtacaacag gcgtaaacgc tctttgacgt aatcaaggcg tgagcgcatg
    18001 gtggccgcgc cttcctcgta ccagtcatat tcgctgtttg attccagagc cttcagttgt
    18061 tccagtgctt cggcttcgag attcaaaccg ctgcgcacga accacttctc cgaaataaaa
    18121 cggtcattcg gcatgttgga cgggaaatca taattattga ccgacatcat gtacacgcgg
    18181 tcccattcca gcccctttgc cttgtgcatc gtcaccacca ccaccttgcc gcgatgccgt
    18241 tcagggtcaa agccagagtc gtcgtctgaa aagccgatga aacgccgttc gttcttcgcg
    18301 atcacggcca gttccgaagc caattcaggc aggcgccaat ctgcatgatc gtcggcggcc
    18361 ttgcgtaaca ccaacgccag tttataagcc aacgccagat ccgaagcctt gccaaacaca
    18421 tcctgtgcca gcgtcagcac caattgatcg atcggaagca cagccgcatt cagccagcgc
    18481 tgcacattga ctttgaaaag gcccagttcc tcgatgactt gcggcgaatc cacctcaccg
    18541 atggcggcca gccattcatc cgcctgactc ggcgcgatga aattttccac attaaccaac
    18601 ctgcgtaaaa tggcagtgac ctctttcaat aactcggggt cgctttcccc agcccttgaa
    18661 tcatcattcc ctgttgcctg ctctctccaa tcccttcgcc acacttcata cgccttcgat
    18721 aacttccgcg cagattgcgg gtcggcaaga tacgacagca gataattcaa cgagcctgcc
    18781 gccgcgcggg tttcggttgt gctggagatc aattcgatcg gttcgatgcc gcgcttacgc
    18841 agttcgttga ccacttccac gccgcgggca ttacgcggaa caaggacggc aatggtcggc
    18901 ttttcttcat cggggatttc tgcaatcgaa gcgacaaaat ttttcaccga tttggccacc
    18961 gcttcaagct catcttcagg ggaatatttc ctgctgatga atttgatgcc ctcggggttt
    19021 tcgggcgggt tttgctgtgg atcgtttttg gggacaggga tgatatatgg cagcgatagc
    19081 gccgtccgca cgttggggtc gggatgggcg ttgagcaccc agtcaataag atgattcgcc
    19141 accgcaagaa tggacggctg cgaacgcccc gactcgggca tatcctggct tgggttttcg
    19201 cggatgaatt tccgcagcaa ctcgggagag gctgtggtga aggtttcaaa gatggcctga
    19261 ttggggtcgc ccacacgcac ccagttttcg ccagataata aagccagtat cctctcctgg
    19321 gtttggcttg aatcctgtgc ttcgtcttcc aaaatgtagg ggtagcgata acgcaggcgt
    19381 tccagaaatt cttcgtcgct ttcgagcagg gtcaacgcat agcggatgag gtcatcaaaa
    19441 tccacggcgc cgcggtaggc gagggcgcgt tgataatccg cgtagatgct gtagccgagt
    19501 tcggcgagcg gcagcggggc gaaagatgca tccagtttgg ctcgcaggct ttcgggagtc
    19561 aggaggcggt ctttcgcaga gcggataaat gccagggcaa gagaatccac cagatcgggc
    19621 agttgtgaac ggcgaatcca atcctgcttg gaattgtcga gggcggggtc gagataatca
    19681 tcgagcgagt tggatgccag ccaggcattg accgattcgc ggcggataaa gcccgcttcg
    19741 cgctcgtcga tgatgctgaa gcgttcttca agccccaccc gcgcaggttt ctcacggacg
    19801 atgtcgtgcg cgagcccatg cagggtgcgt acgcgatatt tgtacaaaga ttcgatgggg
    19861 ttgttgaaaa actgtttgat gcgcttgtcg aaattatcca ctgccgagtt gacgagggtg
    19921 acgataagca cttcctgccc atcttgcagg tcgccttccc tgatgatctt cgccgcaagc
    19981 gccgagagga tgtgcgtttt gccggcgcca ggcacggcgg caatgccaag ccgtccgccg
    20041 ttgtagcgga ggatgttttg ttgggaaggg cggggagtga annnnnnnnn nnnnnnnnnn
    20101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20161 nnnnnnnnnn nnnnnnnnnn ntcccgtgat gcggtttagg aggctggaga agtgcagggg
    20221 tggaaagttt ttatcctgcg ggaagaagat gatccacgcc tcgttgcttt gaccccaaat
    20281 gcctacggtg atcttcaatt tgtctgtcgt gaatacgact tcgctgtgat aggttccctg
    20341 tgattttgtc acagtgactt gaaagccgaa gtccttcacg aggaaatcga aggctttttg
    20401 ggccatgtcg atgaattcgt agggatcact cattggccct ggtgccttat ttcgccgctc
    20461 ccgaatccac atccacgatc attcctgaag tatccacggc ttcgaaatca ttacccgtca
    20521 atacatcaag caagtgcagc atatcgttgt cccagcggtc atcgggcgcg ccgctgatgt
    20581 accagtttga gccgttgtct gccaaaatga ttccatatgt tttcatggct tgcagaatga
    20641 tttgcatttc ctgcgggaaa cctgaaatat cataatctgc tttcaaacga aaacgcgcac
    20701 ccatgggcgg aatcccatcc tgtggatcat ctgtcagatg ccgcgcgggc cagatgtagc
    20761 cagccgtttt ttcgatcgta aaacgcagcg cgtgtttaat ttcgcctgca gcgatttcat
    20821 catagcgcac caggccaggc agaatcggca gacccgccgc gtcggaggat gtccatgtat
    20881 cggggcgcag agtattcgag ttcaaatccc agatcgcacc agagccgccc ctccattgtc
    20941 ctccatcgaa actggcatca aaaacttcgt aaagtgtgca agtatcggta tccaaaacca
    21001 aaatgtgacg gtcgcccccc gcttcctggt ttggattttc aggaatcgga tacggacctg
    21061 gatctgattc gtctgcgtaa tcaaactcga atccaacttt cgggacagct gatccagcca
    21121 cgatattaaa tgggattccg atcaagccgc catcatattc gcctgagccg aaatccatgt
    21181 ggaaacttac agtgcggccg atactgtcga tccacgcctc agattgtgaa tgagtcggga
    21241 gtgtatcgat gggtgcgttc caataattgt ctgcggggaa cattgggcag tttgcgagag
    21301 tcggcccatt cgaaggggaa ggcgcagtgg ttggggaaat ttccgcagta gcggttgcag
    21361 gttgttgggt ttcgacacga tctggtgtgg gcgtgatgct ggcaggcgtt ccaaaattgc
    21421 aggcatacat caatataaaa aaaccgataa gtgttgggat ttgtttgttt ttcattctct
    21481 ctcctgacat cgtataacct ttaaagtgtt cctggcaatt tcccgcgctg ctcgaagcct
    21541 gtcttgttca ttttaacatg gtgcatgcta cctgattttc gtataacttc cgcacgcaaa
    21601 aatggagtaa tgctcatttt cgtacaatgc aggtcgctgg gttttaagcg tggcaaggct
    21661 ggaccaggcg gggtcgagat cgttgaagat caacaggtcg atggaatatt tttggcaggc
    21721 ggtgtcgatg ggctgccagt ttgaaaaact ttcggtaaac aggaacgcga ggtcatgaac
    21781 aaattggtcg taggttgtca gggaaacgcc ataaatggtg cggtcggaga tcaccatttg
    21841 atgcgtgcca tataacccgc tgggacggtc ggcaagggga agcggattgt tttgcatgat
    21901 gacatcggcg gggacctgat cgcgcagaaa atcgtaggcc agccgcgcag aatagccgcg
    21961 cctgccttcg tccacgccga cacgcagggg ccaggtcact ctcaggaaga gggcatccac
    22021 agaggaggtc aagacgccga ggatgagcaa tgtcagcgca agattttttc ttttgacaga
    22081 atcagctttc ggtgagtcag aggcggactt cgtcgaaaag agagtctcga tcacatccac
    22141 gccccagatc agcaaaatga attgaccagg cagccaaacc ctccacccga ggtcattgtt
    22201 tgcgatggtg gatctcaggg tcgagccgat gagcaatacg acgctcatca aaaggacctc
    22261 aaccagataa aacggatcgg aaacgatctc ggtcttattt tttgatccga cccagaaata
    22321 acccgcaaca atgaaaaagc caagttcaag aaaataattc aagggcagca gagccagcat
    22381 gagcaatgag cgcgcgagcg gcggccatgc agtgacaaaa ccttcaagca agtacaaagt
    22441 gcgcacatcg aagatcaccg tggattgagt taccccgcct tcgccttgcg gttgaagcag
    22501 cccgaggatg aacgagcccg caaggagaac agccacaaca ccgcaaaaga tcatcggcac
    22561 gaccaagctg cggtccgttt tttgaatgaa caacgcgagc acccaaactg cccaaaatac
    22621 gccgaataaa aatgtcaccc agatcgaaag cccgaacgat gatgcaaagg ccatgcccgc
    22681 gatcgtcatc aaggtcattt ttcttcgcaa tggtttttcg cgcgccgaaa gcgccagcag
    22741 gatggcggtg aaacacgcca ccatcgctgc aatgtgatgc ggcgcccaca gaatggatga
    22801 attccaggtg ggaatctgca tttgcaaatt ccagccttcg accgtgccat agatgcggcg
    22861 cacgcccagc atgatgataa ttgtggggag gacatcgagc ccgctgacgg ccagcaagct
    22921 gaccccgatt cgtgccgacc tccaaactgc cctgcccaaa ttgacatttc tgacccgaag
    22981 ataaaaagcg atcgccgcca tcagccccag ccctgaccat gccgaactgg cattcagcgc
    23041 ggcgcgcgca tcaacaagag tcccgccaag gatatccacg agactggcga ggatgtacca
    23101 gaaataatat aaaaatgtca gcagggtcgg cgcaccaggg aagtaacttg ggttgatcgg
    23161 cggcacacct gtgcgggtca tcgcatcaat gaccgagacg cgtgatgtct ggtcgaagga
    23221 gaccacgcta acataaagct ggtcgcccca gtgaatatcc accaatgaat aagcgacaaa
    23281 caccacccag ccgatgcaga tccacacaag cgatttggcg gtcggggcga tttgcaggct
    23341 gaaattttct tttgcaagaa aaaagagaaa agccagcaca aacccgccaa tgacgatgat
    23401 ggaaaaggtc attgagccga ggctggaagt gaggttgaaa ataatgggag aaaccgcaaa
    23461 tgaaacgagc aatccgatcc cgaatttgac gatgggaaga cggcgtttga aatcgaacag
    23521 gtccagtgcg gagctgaaaa catacccagg cagcacgatc accaatggaa acagaaagaa
    23581 cgcgagtgtg taaccgatga ggtcttgcag ggtgtaattc agggaataaa aagacaaact
    23641 gccctcgaga atctcttgat cgccacaaag ttattttctt tgaggcgtgg tgttttcgtg
    23701 cctttgtgat tctaatgttt gggttgtgcc ctgccatttt ctcacaccga aaccgagtgc
    23761 ccaaaaagcg gccagttcca tgatccgatt cagcgtagac atgttcaacc gcagtgaaaa
    23821 gccgccgtag aaatctcggt acttttccac gtcccaatcg gggatgtgca tccagaggcg
    23881 gttgaaaatg aaagtgacgg caagcatgag gctgatctga aggctggaga tggcgggtgt
    23941 aatgaggccg atcaaaataa tttgcggcag gaagaggaag gtggcaaagc ggttgaaatc
    24001 ggtgccgccg aaaaaactga gcagcaggac gagtagcgtg taggccagta catatccgcg
    24061 ttgattttca ggcagggagg cgaatgcggc tttgatggct ttcaggtcgg cgaggaatag
    24121 caatggcaaa agataggcga aaatggagaa gatgaaattc acatccacgc ttgggatgaa
    24181 gggcaaaatg accgtggtga gcagggcatc gcctgaaagc ccaacgagtt ggtaattttc
    24241 tgtcacaggg atgagcaggc gcggcaggag aatggcgggg acgaggcaga ttgttgaaat
    24301 ggcggcttgc ttgatgaaaa gtccacgctc tgtggtgcga aatgacataa ataaataggc
    24361 aaccatcgga atgatgttga attcccgaat ctgcgaggca atgagggtgg tcaacagcaa
    24421 tggcaggaat tttcgattga atgccagata ggtttgcaaa agaatgagtg cgtaggccag
    24481 gtggtcgggc ctgtaaatat cgaaaagcag gaacttcaca ttgaacaatg aaagcgctgt
    24541 cactgccatg gccagataag cgcctttggt cgaatttccc agccatttga tgaaaaagaa
    24601 gattcccgtc agctgcatga tcgcaccaaa ataggcgatg atgccgaatc cgcgctcgat
    24661 ggagaatccc agcagcgaaa tcccgtggac aatgaagggt gtcaggtagc gatacgcaaa
    24721 cggactggcc aatgggtcgg cgtgaaatcc atctttggca agcgcaatgt aataaaggaa
    24781 atcccaggca tttccctgcg tgttgatggc attcgtgaca agatcaagcc ccagcgtcgt
    24841 cagagccgcg aggaggagaa tccacaggag ttcgcgtgtt ttcattccaa tccaattttt
    24901 ctggaatgcg tgttgaattt catcctggtc ataaaaccct gcggactgaa gtccgcgtta
    24961 caggaaatga atttcattcc tgtccctgca gtgacaacac tttttggaac gcacgcaata
    25021 gagaaccgcg ctgttccatt ccagattcgc ttaaatcggc cagcgccaga aatacttttt
    25081 ccctgcatcg gctgagcaac ccgccgacca gttttgccag tgcgtcggtc tcttcgcgta
    25141 cctcatcggt gtccatccag aaatgtgctg gtgacgggtc ccatccacgc gaaagcacgt
    25201 agggatgagt cagcggctgt gaaaggcgct gaacccagcc cacagaacca ggatccagcc
    25261 aaaattgaat agtgactggg tggttcatca tcaaaaaggt gtgagcgggt gcaaccaaca
    25321 cagcctcttt atcttcgctg cgccatgatt caagatattg tgctgcgatc acgccatctg
    25381 ccagcatgga gaggtattcc ctgcccatgt cgaaccctgt ttgatcaaag ggctccatcg
    25441 cgttgcgaaa cttcctgatg gattcgatca gacttgctgc cacacgcacc gcatccatgt
    25501 tgctgtggaa tccgtattcg ggctgtgaga tcacttcacc gaataatttt ctcaaaaaga
    25561 aatccagtgg caggggagcg gcttcacgat attcgagcag ccagttgcgc agggtcgtgt
    25621 atttttctcc gagcctgtag gtgatgcgct cctgcatttc aggcctgatc tcttcaaaag
    25681 gcgaaagacg cagatccctt tgtccataca ccacttcggt cagcaattgt gcgcgcacga
    25741 gatctgtgtc caatgcgaac atcagggttt gcatcatgtc aaattttgtt ggccgcacat
    25801 tccagtgcgg gtgtgcaagt gctgaaagat ttaagagcgc attgctggca ggctcatcgc
    25861 gcagtgaacg cgatggacga tgcgagcgcc atggaatatt ttccttttcc aaacggtctg
    25921 cgatcgagaa acgcaatgcg tctgaaagat acggcgccag caccacgatc tcggcgggtg
    25981 gcacaccttc ttcgatcagc gtttttattt tgccaaccac ctcattcaac atctcagggt
    26041 agaagtgcgc catgatgaat tccatcgtgt ccggtgggta ggcctcttca cccgtgcttt
    26101 gatttgtgac cgcagaaacc agtgaattgg aaagctggat gatgttttcg gatgagacga
    26161 aactctcgct gaattcgatc tgactctcac aggtctcgat cagggcataa ccgctggctg
    26221 catcgcctcc gaggaagcgg cgataccccg catcattgtc gtagatcaac agcgccgatt
    26281 cgaaatctgg catccactcg cggatgatgt catgcgcgcg cggggcatct tcttcaatgt
    26341 tgtcgtagat gaggtggcgg taggtctgtt tgaggtagtt ttgaacctgt tcctgtggcc
    26401 agagaatgtc agtgaatatc tccagttgaa gggagaaatc gagcaggttg tgttcaaggc
    26461 aatattcacg gaaacggatg gcacaatcct gcacatcttg ataaatgcgg cgctggcccg
    26521 ggtcgccgac ccaggcggca tcaaggcgtg tgccgatctc tgtgtaaggg aaaccaacca
    26581 ctgccgcctt gttcagggtg tctatgatct gcgaatacat gcggttgcgg tcaatggtca
    26641 gggattgaaa gtagccctgt tccagcaacg ggcgcacaat gaatgccatg taatattgtg
    26701 cagtttcaag agtgagaaaa acgggagccc gctcaggatt tttgacccca gccgcttcgg
    26761 cggcgatggg ccagaataga tcgcacatgc gtttggcgag gccgccaatg gtggcggaag
    26821 tcacttcacc acctgcacgt cgctccgggc tgtaaagcaa ctcgagatac ggttcttgca
    26881 gtgtgcgctg tggtgtgagc atcaggatcg agtcggcggg gacgccttgc tcaagcagga
    26941 agcgcatccg ttcgacccct gtggttgtct tccctgtccc tgcacgacct gagacgaaga
    27001 tgcgcgagtc gaggggtgac tcgatgattt gacgctgaag caggttgagg ctggccatga
    27061 agcgagaata aaggagagcg gaagatttgt caactggatg taaatt
//
LOCUS       DAOQLC010000059        26016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_621_length_26016_cov_23.919341, whole genome shotgun sequence.
ACCESSION   DAOQLC010000059 DAOQLC010000000
VERSION     DAOQLC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 26016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_621_length_26016_cov_23.919341"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="PL000_11695"
     CDS             complement(<1..1484)
                     /locus_tag="PL000_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HNC89609.1"
                     /translation="MSKFDRRSFLKLIGAAAPAMLLPSLAHRIGGLDASRPNIIVLLF
                     DAMSARNLSLYGYPRPTVPAMERFAEHANVYHAHNSSGNYTIPGVASLMTGTYPWKHR
                     AINHRGAIRQSMVENNLFRVLGSDYHRLAFPQNFWATLLLSQFQDDIDVLLSSGEFSE
                     LSYLLSDHFPKDRNMSARALDDFLFRVGEAPPSLVLGSLERLLYTWQSNHLPTDGYKE
                     LPHDLNYPITFRLEDVFDGLISLIPSLPAPFFTYLHLLPPHTPYRPTDKFFNAFRDDM
                     KPMRKPDHRFADGTSNADLNLARRLYDEYIASLDQELGRLLDSLESQGLFENSYVILT
                     ADHGEMFERGEKGHSTPLLYDPVIHIPLLISAPGQKTRRDIYTPTNAVDILPTLAHLT
                     GNPLPAWTDGKPLPGLGGEEDYERSLYSVEAKLSPANGILNKATVALRKGDRKLIYYT
                     GYEAEDSFELYDLSENIEELHDLYPDQPAFAKQMKEELLEAFFEA"
     gene            complement(1528..2061)
                     /locus_tag="PL000_11700"
     CDS             complement(1528..2061)
                     /locus_tag="PL000_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89610.1"
                     /translation="MDIVKKIPRWEQVIPVYAVIVMMVYVWSLFQYFWRLSSWLNFST
                     LGQVGGIYVYTVAVNFVESLLVLLVLLALSAVLPATWFSDEFVVKGSTLTLLVLGGLM
                     VFNQTLFEDVLHPSILLPKVLAFAAVTAFLVFLFGRVGFLKKTMEEVANRMTVFLYLW
                     MPITAISILVLLTRNIF"
     gene            complement(2162..3190)
                     /locus_tag="PL000_11705"
     CDS             complement(2162..3190)
                     /locus_tag="PL000_11705"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC89611.1"
                     /translation="MLKRFKLAAKILLRGEPQKKNRNPIPAITAEEVAEARAFFPRDK
                     FFIFGHARSGTTLLMRLARLHPEIHCNYQAHFFTRKPLLKSLVDSPEAEEWLTRKSNR
                     WNQGRDLSPLVLRAAADFIMERDAAREGKMIVGDKSPSSTIHGQAVRDMHAVYPDAKM
                     VCIVRDGRDVLISERFRNFVEESRFLTSEDKRIISDLRLDPVPFSDGRRSIFTETFIR
                     RVAKDWVNDLTETEGEGRRLFNDRYFGMRYEDLLAQPFDEMSKLWKFLSVQSVDPSLD
                     SSLRAEMSTNPDEEWQSKRNEGIASFLPKGQAGNWARLFTERDKAVFKEVAGEMLMHW
                     KYEKDLNW"
     gene            3570..4517
                     /locus_tag="PL000_11710"
     CDS             3570..4517
                     /locus_tag="PL000_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89612.1"
                     /translation="MPPSVTTQVAEVAPLEVCTPVPSENVNVYRTIPQPWAGLIFDYA
                     HATLPATSPTLDPLLAAAFPAITQLQGAQYAAFQKLVSETKRWSDTETIKLDDAENKV
                     RITLTFISPQLLQAVYLNQVLKDGYPASGFLEQLQSMLNNVAARDELLFLLTISPLSS
                     INPIHHTVELPIDSIVLNNSENLQVSHSHDDHNLAQPFDTSSDAIFGYLAFPFTQLVN
                     SECKWILNPKFNTNIVVTMPYLKVDGNKNGISYSWTIPYTSLINQTVTLDALNFNPPP
                     GYDFTQVTPSSLPPTDISSPSSYDESFAKFIWKQITGSY"
     gene            4521..5531
                     /locus_tag="PL000_11715"
     CDS             4521..5531
                     /locus_tag="PL000_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNC89613.1"
                     /translation="MKTIFTFVPSKTHEFPDHPERPGRLDVLEPLLDSVDAERIKAVP
                     AAPEQIALAHKPQLVKAIAEVCTRGPGIIDHAPTFITQTSYEDALLAAGGVVTCTRAV
                     LNGEAQNAFAIVRPPGHHAEPDRAMGFCIFNNIAIAARDALANGLQRVMVIDYDAHHG
                     NGTQAVCVDDDRFGFISTHQWGIYPGTGWINDAPHAKGRLVNVPLHEFAGDGTFARVA
                     DEIFKPIVESFRPQMLLISAGFDAHWNDPITSLGLSTRGYYEISKKLVALANEHCGGK
                     IVFVLEGGYDPHNVANGAMAVFDALTNSPLNKDAGDPSPYPDSDHESRIHDILKWHGI
                     AQ"
     gene            5575..6651
                     /locus_tag="PL000_11720"
     CDS             5575..6651
                     /locus_tag="PL000_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC89614.1"
                     /translation="MQRKQSKGFVELEWECPNCHSANKGSKKICESCGAPQPEDVKFQ
                     RAADEKIITDEKVVSAASAGADVHCGFCGTRNPATATNCSQCGADLKEGKARQAGQVL
                     QAAPESAKIITCKSCGTENPGGERLCNKCGAPLPRAESVAAKPAAAVAANSGTSSAAA
                     PKAQPKKTNWLLFGAIGAFLMVCCIAIFAMFVIPSKSVQGTVADVYWQTSVPLQEVRA
                     VDYSNERGNPPAGAYNVSCHTESQEICEDKTVDRGNGFAEVITECHTESQDYCSYTVD
                     EWTTIQSYDLSGSDLFPEYAQPSVFNGQRLGNASATLTVTFDTPKGTITYTPGTVSEF
                     QQFQIGSTWTLKLNALGGVLSVGK"
     gene            6662..7165
                     /locus_tag="PL000_11725"
     CDS             6662..7165
                     /locus_tag="PL000_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89615.1"
                     /translation="MTDRAEDSLKNDVLYTDDLALNRNGQLSETQKKRLWPGALFWLM
                     LAGINVIFLLLLSFFQLRYQTVGCGYLGLFVIAIFSIRQCILNSVPYREDIKNGIVKT
                     ASGQAFKRAVLQSFSKGVYLGNCSVRVNNKVFTISPHFYNWMVDEGYYRIYFTPETKR
                     VVNIEEL"
     gene            7254..8468
                     /locus_tag="PL000_11730"
     CDS             7254..8468
                     /locus_tag="PL000_11730"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNC89616.1"
                     /translation="MAIIGGGPAGLMAAEVLSGHGMQVDVYDSMPSLGRKLLMAGKSG
                     LNLTHSEPFEKFVLRYGSKKQEVETWLNDFTPDDLREWARGLGVETFVGTSGRVFPKE
                     MKASPLLRAWLKRLTAAGINFHLRHRWNGAIAAGEGGGSIVKFETPDGIKTVHANAVI
                     LALGGGSWARLGSDGAWVEWLDQAGVKVEALKPSNCGFDVAWSLHFREKFDGQPLKSV
                     TVSFGDFHQQGEFIVTREGVEGSLIYAASALLRDEIAQNGRAILELDMSPDRPLEWLV
                     ERLSKPRGSRSMSSHLEKTVGLKGVKAGLLYEFISKDDFANTERLAFFIKHLPIPLIA
                     THPLDEAISSAGGVSFKAVDENLMLHKLPGVFCAGEMLDWEAPTGGYLLTACFASGRV
                     AGLGALKWSNAG"
     gene            8490..9131
                     /locus_tag="PL000_11735"
     CDS             8490..9131
                     /locus_tag="PL000_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC89617.1"
                     /translation="MKNLDYRDYYRRNLPHFQPRGATFIVNFRLAGSLPVEVVKMLKA
                     ESEEIEKSLLRIEDHTERYHVKDREQLRLFSKWDEALHNSQAGPFWLKDERIAQIVVN
                     SILYHDENWFDVLAYCIMPNHVHLILAPRENADADFSLAKIMHNIKRNSANHANKILE
                     RTGAFWQHESYDRFARNEAELERMIKYVLYNPVKAGLVDDWKKWKWSYCKFEM"
     gene            9173..9604
                     /locus_tag="PL000_11740"
     CDS             9173..9604
                     /locus_tag="PL000_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC89618.1"
                     /translation="MHEDILKIQQLIARFANSFDVKDWDGLQACFTESLYTDYSDLRG
                     TPPETISASEYVRLRRESLDHLKLHHLVSNYEITFPGFDVAACRASMVVWRRSEAEDF
                     TTHCIYDFQLARQRGTWKISGITQKILWNDGNASIHKGAKN"
     gene            9607..10542
                     /locus_tag="PL000_11745"
     CDS             9607..10542
                     /locus_tag="PL000_11745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC89619.1"
                     /translation="MYRKGWRYYRDATLLWILFFVVLMIGGYALEHPLYWILALFVWV
                     VRSAFGNAFQFAHPSRRFNWDSRSNLVFKEVTFKSRDGLTLFGRFHAGRTRATIILIH
                     GLGGAGTDMLVHASFLVDAGYGVFMIDLRAHGSSDGDTSTYGLREADDVAGAVDYLLS
                     RMDVNGAKIGVLGISLGAQAALRGALKTENIRALVLDGLSPARLSDHGGSPKSIQRWL
                     NYPFNWLYYRIYEYMIGGRDAGVLEVIGQIAPRPILLIASGLRDIYFSRLFYEAAKDP
                     RALWEIPDGIHGVALAGNPQEYIDRVVGFFNQTLL"
     gene            10539..10964
                     /locus_tag="PL000_11750"
     CDS             10539..10964
                     /locus_tag="PL000_11750"
                     /inference="COORDINATES: protein motif:HMM:NF039927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HNC89620.1"
                     /translation="MTACPECGATNCESRFHEFLALEFSNAAYGNVHHLTVAAYMLQH
                     SSKLTREGWRYERELLRAFLVENKTPAFIRRQNRDWVDGGKRTFKIKSKDGSPFINRS
                     AWTKTILNVRAENAEVYCADITTWAGAVLKDAEEIKLEI"
     gene            11108..12499
                     /locus_tag="PL000_11755"
     CDS             11108..12499
                     /locus_tag="PL000_11755"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HNC89621.1"
                     /translation="MKRALQTSFIIWTILSALVLASVIVIYLRNGETGLNLFIQQWPL
                     SNLTVTLEATAYTWLCAGAILYLLANDKVNAENAFFVAGFFIVILLYLNVLRERFRYG
                     DYQYYLEAATALMNNQPLPDTYLYLPLWATLLQFIVPLGDQGMLAILWTVNIIMLGSF
                     YILLVRVLEHYGYSNRFAVIIAVLFMSINTPLHRSLGYVQVNLIAMVLIMASLLTFRK
                     NDLVSALLLALAVHLKTSPAALALAFLLERNWKWLAWFALTFILIALIPVALEGTRPY
                     FDYLTNINILTHITDTNFHDTSFDSFFRFFNPFLGINLEATRLITMGAKALLGLAAIY
                     VMAQNIRSHTFVSRETQNHILLNAIPSLFIVMTLTSPIVWDHHGIFTTLAFLLLLHCI
                     ETPALWMWFGFAYFLEFILPSFDFFPWSFGRLIAPLIVLCLMWYVTRSKQSPSLLVSA
                     NRWFENLKFQISQ"
     gene            12496..14241
                     /locus_tag="PL000_11760"
     CDS             12496..14241
                     /locus_tag="PL000_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89622.1"
                     /translation="MKQQRPILILLAVSLLIGIFTLNGYGDSWDDLSLRKYAAKSLEA
                     YGTWVARGEVQITTEDLGSYGPSYVMAVALLSQDNPDLRHFIYFVTFLAGAWAFYMLG
                     LRWLSQPAALGATLLYLTQPVLWGHAFFNPKDTPFLSLFLLSIYFGLKLFDESPNPIQ
                     GSDRRTALLTALWLGSVFSLFLLTDTFHTAIVNLVHAAKAGETNIISLTASDIRTAAP
                     ELYIQKYFTLFLRLRAVFFLLSSFFFLFHKSFRSLHPSAFTRSVYPSVFLGFTSSLRV
                     LGPFAGLIVAYYALRKHGRKSIPTLIAYAVTAIIVMYLTWPYLWTNPLGNFIASIKTM
                     SLYPWSGQVLFNGQQFASTELPASYLPVLLGLQLTEPLWALTLIGVVVAGIRLREKRE
                     LIELSILWLILPLFGFIVMRTALYDNFRQVFFILPPLFWLAGVAIEQVKRPAFQMALI
                     VLAALPGIVDGIRLHPYEYIYYNRFIGGVDGAQGRFELDYWGASYREAAEFVNTTAPA
                     NATVWIEGPSQLFELYAREDLKLYSTHEADRADHYDFAVALTRDHMDQASFPNAEVVY
                     RIMRGRAVLAVVKTP"
     gene            14329..16179
                     /locus_tag="PL000_11765"
     CDS             14329..16179
                     /locus_tag="PL000_11765"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HNC89623.1"
                     /translation="MNFLELLSQKTVVADGAMGTMLHAHGVDFGKCFDELNLTNPAAV
                     ADIHREYIEAGAELIITNTFGANRYKLKKNGLADKVKEINHAGVELAKRIVAASFKEV
                     LIAGDVGPLGVRIAPYGRVKPEEAREAFAEQIRALAQAGVDLIVIETMSDLYEIQEAI
                     KAAKQACTLPVVASVTFTRDDRTLLGDDPMKVARALHGAGADVIGVNCSGGPSQLLRI
                     LKQMRQAVTDRSAKFWVKPNAGWPEQVGGRIMYPADADYFGDYALAFREAGANIVGGC
                     CGTTPQHIAAMKKAFATTPVQISEMWEASEVLETESQDVEQPTQLAQKLAAGKFSICV
                     EMDPPRGLSTHKLLAGASLLYDAGADVINVADSPMARMRMSAWAVCDLVQRQIGVETT
                     LHFPTRGRNLLRVQGDLLASHAIGLRNIFVVMGDPTSIGDYPEATDNYDLVPSGLIKL
                     IKQGFNAGVDHSGTSIGQPTNFFVGAALNLCPQDMDTEVKNLHRKIKAGADFFLTQPI
                     YRADDGPKLIEAYEAKHGKLQQPILAGILPLVSAKHANFLHNEVPGIFIPEEARKKIE
                     AAGDAERGVKIGVELAVELIEQIKGWANGIYVMPQFHRYDMVAEIIEKIR"
     gene            16228..16653
                     /locus_tag="PL000_11770"
     CDS             16228..16653
                     /locus_tag="PL000_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89624.1"
                     /translation="MWKKTLQKIILILLVLLGIKTGVQGLASTINMPSHDVSVAKWDE
                     RAAALKDALPFKRGFVGYISDIDIPNVEYDRQNLVGEFTLTQFSLAPIVLIQGSDDEE
                     WNILSLSQQAYEIWSAENAAQYEVIKFGGKFYLAHKVTP"
     gene            16650..18050
                     /locus_tag="PL000_11775"
     CDS             16650..18050
                     /locus_tag="PL000_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89625.1"
                     /translation="MTAALSLLSFALPMLTGILLIHALWAEKNNFFDWVLKLSLGTGI
                     GLGISSLLYFVYLLAFAGKPWFLFVEVLTFGAVIIATRKYLRVSVSLWFKPTRAQTWA
                     LILAGIVFFTTVAGILNYSRQRALGDWDAWMIYNRAARFLFRDQAAWQESFPPEMSVI
                     FHADYPLLLASNNAARWDILNKETPYVPMFQSVLFAFAALGLIFGALGRFTSVGQASL
                     GLILLAGVPFYLLEGGRQTADLPLAFYFLATVVFIFAYYEEKRFSLLFFAGFTAALAA
                     WTKNEGLLFLLIAGAVILLLRTADFSLRIWHKQVFQSFLPFFAGALLPLSVALYFKVA
                     LAPASEFLSGGLTKILQDIADPARHGIILAYFKNIFLFSNGWYRVGILPILCVYFLVF
                     HTRKAQGNNPVFPGFAIFTLQIIGYYAFYLISPYELDWHLSSSIDRLFIHVYPTILFV
                     TLAASQTPETIFESDV"
     gene            18143..18907
                     /locus_tag="PL000_11780"
     CDS             18143..18907
                     /locus_tag="PL000_11780"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC89626.1"
                     /translation="MLLTIDVGNTNLTLGLYEGEQLGAHWRLATDHNRMPDEYGLQFL
                     GLLQNAGKTLKDIHGISLASVVPPLTARVIQACREYLRQEPLIVDAGVKTGIKVRYED
                     PKAVGADRICDAVAVMKLFGGPACVVDFGTATTFNAVTKDGQYLGGAITAGVNLAAEG
                     LFTHAAKLPRIDLQRPPSVIGRNTVHAMQSGLLFGYVSMVEGMVARFRSELGSDMKVI
                     ATGGMAEVIAKETQVVDHIAPWLTLDGLRIIWELNQ"
     gene            18915..>19921
                     /locus_tag="PL000_11785"
     CDS             18915..>19921
                     /locus_tag="PL000_11785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC89627.1"
                     /translation="MKKIFALFLGMSILLSGCGTGLFAEPTSTPTMTFTPPPPTEAER
                     ATPTQTFTITPTRFATASPSPTPTWVHQGPESVEVPILLYHWIAVSPDEGTNYKSPYY
                     VKPEVFDAEMKLLHDWGYTTITTEMLIKAIREGADLPPRPVIITFDDGHMNNYTNAFP
                     IMQKYGFTGVLYLVANYLDAEDYLTVDQIKEMAAAGWEIGSHSVSHSDLTSLEPERQR
                     FEVVDSRAMLESKLGVPVLTIAYPFGVSNSSVIDYAHFAGYIGGMGLGYTHDQGLTNL
                     FNLQRRDVKGTYDVKQFASFLPWQGDAIFLPTDTPTPTMTPTRTPIPTYTWIVPSTST
                     P"
     assembly_gap    19922..20021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20074..20997)
                     /locus_tag="PL000_11790"
     CDS             complement(20074..20997)
                     /locus_tag="PL000_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89628.1"
                     /translation="MKSSSQLSRGYVIALTATIIWSTTGILISYLSRDYSLPSLVLAF
                     WRDLFVSFGMAVGLLVFSRPLFQLDRKHWGFMVWYGLTLAIFNSMWTFSVQYNGAAVA
                     TVMAFSSPAMTAILSRIVLKEQFSRIKIVSILLSLAGIIFVSGAYDPSAWNLNPLGII
                     FGLLSGLLFAIYNLEGKHASDTHINSWTALLYSFAWATVFLFFFNLGNDLFLTGKGAF
                     ADLLWLGDSVSGWGWLFFLGVAPTLGGFGLYTLSIRYLSPTTSNLIATLEPAFTAIWA
                     YFLLNEVLTGMQLFGGALVLMGVVLLRFGEK"
     gene            complement(21052..21124)
                     /locus_tag="PL000_11795"
     tRNA            complement(21052..21124)
                     /locus_tag="PL000_11795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21088..21090),aa:Glu,seq:ctc)
     gene            21234..22184
                     /locus_tag="PL000_11800"
     CDS             21234..22184
                     /locus_tag="PL000_11800"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89629.1"
                     /translation="MTFPSFLKLTPEYRDYVWGGEKLRPGHSPTAEAWVVYENDRIES
                     GALAGKTLSEAANEFGAALLGEKVFARTGTRFPLLVKLLDCAQWLSVQVHPNDEQAVQ
                     LEGPGQFGKTEAWHVLEAQPDSKLIAGIQPGASAAEIAEAIRNGTITEYSQYASVQQG
                     DTVFMPAGTLHALGPGLLIYEVQQTSDWTYRVYDWGRPQTETRRLHIDKSIAVTRHDL
                     AAPIISAPPSGDGTRHILTQCEYFTLELISASTRTVELDTQKESFHALTVIEGRAELV
                     SATETVTLEKFQTALVPASLGSYQLRPLEEEACRVLKSSV"
     gene            22468..23820
                     /locus_tag="PL000_11805"
     CDS             22468..23820
                     /locus_tag="PL000_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC89630.1"
                     /translation="MTINDKTVTIIGAGMAGLSAALDLHRAGWQVTVLEARNRVGGRV
                     HTVRSFSNGLTAEGGGEYIDEDHTRMISLAREFNLSLGKVGSWQSQNDDWGAFEGKAG
                     SIADSTLWGTNLASEIHNMWQAVAELGKEVNDPSNPQTARRAEELDHRPASEWIAAQP
                     VHPLARKAFIQHIRSEYTCEPEAFSLLDLARNASFYYSDAEAWPQTHRIIGGNDQLPQ
                     AMAARLPDVRLNAEVVSINLQPQKVTVAYKQGEVYHTINSVFAILAIPLTTARLIDFK
                     GSLPSAHQKMVNEISYGKVTKVMIEYRNRFWKDKKWNGRLTTDLPIVLTWDATSHLEG
                     ERGILTAYTGGGPGAYLTGLSDEERTKTAIAVIEKIFPGSSDLIESTATIAWGNEPFS
                     RCSYMALAPGEVTAHWKTLFSPAGRLHFAGEHATPYQGYMEGAVESGQRAAKHIMESV
                     "
     gene            23817..24518
                     /locus_tag="PL000_11810"
     CDS             23817..24518
                     /locus_tag="PL000_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HNC89631.1"
                     /translation="MSESSKAFWEGVRAEFPLLIGVFPFGMIYGALALNAGLSTLASQ
                     MMSSIIFAGSSQFVTAQLVHDAAPGLVIIVTIAVVNLRHMLYSASLAPYLKNLSLQWK
                     ALLSYLLTDEAYAPSVLKYEAEGINASSHWFLFGAGLSLWSTWQVSTAIGIFLGTSIP
                     ESWSLDFALPLTFIAMVVPALKNRPMIAAALSAGGVALLAHNLPFKLGLILAALTGIV
                     IGTLFEGRQDEKGAQ"
     gene            24515..24826
                     /locus_tag="PL000_11815"
     CDS             24515..24826
                     /locus_tag="PL000_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HNC89632.1"
                     /translation="MNIWLVMVIGGLITFGMRFSLIYLFGRFEIPETMRRALHYVPPA
                     VLSAIIFPELLYHANQIDFSFGNIRLLAGIVATLVAWFTKNTLLTILAGMLALFLFQL
                     L"
     gene            complement(24905..25810)
                     /locus_tag="PL000_11820"
     CDS             complement(24905..25810)
                     /locus_tag="PL000_11820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC89633.1"
                     /translation="MKILVLENDPNEFAIIQQAFNDGKTSLIPIASSEQAWPYIQSGE
                     ARFVIANWDTSDVRRSQFILRARAIKLTQPLYILLTMAKITDDDQTPSGADDILQRPF
                     RAKDLKNRVGMAERIISLASNLAVAREQLEHQAVFDSLTGFLNRAAFFKQAMGELERA
                     RRTSMPVSLIALDVDHFKVINDSLGRDIGDQVLHVVAQAIREKSRPYDCIGRWAGDEF
                     VLMLSGVIGADAEKVTERIITGVRSSRVVVHNNTPLNIKVSAGIASVARISTSVEVEP
                     LIQQALQAMKRAKEAGGDQVFLIYM"
BASE COUNT         6169 a         7047 c         6583 g         6117 t
ORIGIN      
        1 gcctcgaaaa acgcttcgag taattcttcc ttcatttgct ttgcgaaagc aggctggtct
       61 ggatacagat catggagttc ttcgatgttt tcgctcaggt cgtacaactc gaaggaatct
      121 tccgcttcgt aacctgtgta gtaaataagt ttgcggtctc cctttcgcaa agcgaccgtg
      181 gccttgttga ggattccgtt ggcagggctg agttttgctt ccacagaata caaactgcgt
      241 tcatagtctt cttcaccgcc gaggccgggc aatggctttc catcagtcca cgcggggagc
      301 ggattccccg tcaggtgggc cagcgtgggg agaatatcca cggcattggt gggggtgtaa
      361 atatcgcggc gcgtcttctg ccccggtgcg gagatcaaca acgggatgtg aatgaccggg
      421 tcatacaaca atggcgtgga atgtcccttc tcaccccgct cgaacatctc gccatgatcg
      481 gcagtaagga tgacatagct attctcaaaa agtccctgac tttccagcga atccaagagc
      541 ctgcccaatt cctgatcgag ggaggcaatg tactcatcgt acaaacgccg cgccaggttg
      601 aggtctgcat ttgaagtgcc atcggcaaaa cgatgatcgg gtttgcgcat gggcttcata
      661 tcatctcgga aggcgttgaa gaatttatcg gtggggcgat atggcgtatg cggcggaagc
      721 agatgcaggt acgtgaagaa cggcgcaggc agactcggaa tcaacgagat caaaccgtca
      781 aacacatcct ccaaccgaaa agtgatcgga tagttcaggt catgcggcaa ttccttgtag
      841 ccatcggtgg gcaggtgatt gctttgccac gtgtacaaca atctttccag agagcccagc
      901 accaaagaag gcggcgcctc ccccaccctg aacagaaaat catccaacgc gcgcgccgac
      961 atgttcctgt ctttggggaa atgatcgctc agcagataac tcaactcgga aaattcgccc
     1021 gatgaaagca acacatcaat atcatcttga aattgcgaaa gcagcagggt ggcccaaaaa
     1081 ttctgtggga aagccaggcg gtgatagtca ctgcccaaca cgcggaaaag attattctcc
     1141 accatgctct gccgaatcgc gccgcgatga ttgatggcgc gatgcttcca gggataggtt
     1201 cccgtcatca acgaagccac gccggggatg gtgtaatttc cgctggagtt gtgcgcgtgg
     1261 tacacatttg catgttcggc gaaccgctcc atcgcaggca ctgtggggcg cggatatccg
     1321 tacagtgaaa gattgcgcgc actcatggca tcgaacaaca gcacaatgat attcgggcgc
     1381 gaagcatcca acccgccgat gcgatgcgca aggctgggca gcaacatggc aggagcggca
     1441 gccccgatca acttcaagaa actacggcgg tcgaatttgg acatcgatat tccttctttt
     1501 tatgtgcctt tgtgtgcgat gtgcttctca aaaaatattc cgcgtcaaca gcaccaaaat
     1561 cgagatcgcg gtgatcggca tccagagata cagaaacacg gtcatgcgat ttgcaacttc
     1621 ttccatcgtc tttttcaaaa agccaacccg accgaacaga aaaacaagaa atgcagtgac
     1681 cgcagcaaaa gccaaaactt ttggaagcaa tatcgaagga tgcagaacat cctcgaacag
     1741 ggtttggttg aaaaccatca acccgccgag aacgagcagg gtcaaagtac tgcccttcac
     1801 cacgaactca tcagaaaacc acgttgcagg cagcaccgca ctcaatgcaa gcagaacaag
     1861 caaaaccaaa aggctttcaa caaaattgac cgccaccgta taaacataaa tgcctccaac
     1921 ctgtcccagt gttgaaaaat tgagccagct tgaaagcctc caaaaatact gaaagagcga
     1981 ccacacatac accatcatca cgatgacggc ataaacgggg atgacctgct cccagcgggg
     2041 gatttttttg acgatgtcca aggggaatat ttccttcacg acttgcaacg cttcacactt
     2101 aaccgaaagc tgattccgtt ccgaggggca ttgccgcgta ccgcaacgtt cacgttgccc
     2161 actaccaatt caaatccttc tcgtacttcc agtgcatcag catctcgcct gcaacctctt
     2221 tgaacacagc cttgtcacgt tcggtgaaca gccttgccca attgccagcc tgccccttcg
     2281 gcaaaaagga cgcaatgcct tcattgcgtt tggattgcca ttcttcatca gggttcgtgg
     2341 acatttcagc ccgcagggac gaatcaagag aagggtccac actttgcacg gagaggaatt
     2401 tccacagttt gctcatttca tcgaacggct gcgccagcag gtcttcataa cgcattccaa
     2461 aataacggtc attgaacagt ctgcggcctt cgccttccgt ttcggtcagg tcgttcaccc
     2521 aatctttggc aacgcggcgg atgaaggttt cggtgaatat cgaacggcgg ccatctgaaa
     2581 atggaacagg atccagccgc aggtccgaaa tgatgcgttt gtcttccgat gtcagaaacc
     2641 tggattcttc gacgaaattc cgaaaacgtt ccgagatcaa cacgtcacgt ccatcgcgga
     2701 cgatgcaaac cattttcgca tcggggtaca ctgcgtgcat gtcacgcacg gcctgcccgt
     2761 gaatggtgga agatgggctt ttatcgccga cgatcatttt gccttcgcgg gcggcatcgc
     2821 gctccatgat gaaatctgcg gcggcgcgca gcaccagcgg agaaaggtcg cggccttgat
     2881 tccagcggtt ggatttgcgg gtgagccatt cttcagcttc gggtgaatcg accagcgatt
     2941 ttaacaacgg cttgcgggtg aaaaaatgcg cctgataatt gcagtgaatt tcgggatgca
     3001 gccgtgcaag gcgcatgagc agggttgtcc ctgaacgggc atggccgaaa atgaaaaatt
     3061 tatcgcgcgg aaaaaaggcg cgggcctcgg caacttcctc ggcggtgatg gctgggatgg
     3121 ggttacggtt tttcttttgg ggttcgccgc gcaacagaat tttggcggcc agtttgaatc
     3181 gcttgagcaa gggttgtttc tcctaaccgc ctgattttac tggaaatatc gccgccttga
     3241 gatatgtggt tcagcgtggg tgtgctaaag tttgtagatg atctgctccc ttcgccatcc
     3301 tcggcgaagg atttactcgc ggaacgccgc cgaggacggc ggcttgagct gaggcccatt
     3361 gagatatgtg attcagtgcc agcttaactt ttaattcaaa cggttgtaca atgattaata
     3421 ttattcatcg caaaatttct tttttttgcg catttgattt cggaaaggcg gggcgaatgt
     3481 ttcgggtatt ttctttgtct aaaaaacgac cgtttatttt tatgtgcggc ctccagacca
     3541 tgttggtttt atttgggagc actggatgca tgccgccctc cgttacaact caggtggctg
     3601 aggttgcccc attagaggtt tgcacacctg tcccgtcgga aaacgtcaat gtgtatcgga
     3661 cgattccaca gccatgggca ggcctgatct ttgattatgc ccacgccacc ctccctgcca
     3721 cctcccccac actggaccca ttactagccg ctgctttccc tgcaatcaca caacttcagg
     3781 gggcgcagta tgctgcgttc caaaagttgg tcagcgagac aaaacgctgg tctgatactg
     3841 aaacgatcaa gttggatgat gctgaaaaca aagtacgcat cacactgacc ttcatcagcc
     3901 cccaactcct gcaggcggtg tacctcaatc aggtgctcaa agatggatac cccgccagcg
     3961 gttttctgga acaacttcaa agcatgttga ataatgttgc tgcacgcgat gagttacttt
     4021 tcctgttgac catctctccg ttgagcagca tcaaccccat ccatcacact gttgaactac
     4081 cgatcgactc catcgttctt aataattcgg aaaacctgca agtgagtcac agccatgatg
     4141 accacaacct ggcccagcct ttcgatacat cgtcagatgc gatctttgga taccttgcct
     4201 tcccattcac acaattagtg aacagtgagt gcaagtggat cctgaatcca aaattcaata
     4261 cgaatatcgt tgtcaccatg ccgtatctta aagtggatgg caacaagaac ggcatatcct
     4321 attcctggac aattccatac acctccctga tcaatcagac cgtgacttta gatgccctga
     4381 attttaaccc gccgcccggg tatgatttca cccaggtgac gccgtcatca ctgccgccca
     4441 ctgacatctc cagcccatca tcgtacgacg agagttttgc aaaattcatc tggaaacaga
     4501 tcacaggaag ttattaaccc atgaaaacca ttttcacctt tgtgccttca aaaacgcatg
     4561 aatttccaga ccaccctgag cggcccgggc gtttggatgt gctggaaccg ctgcttgatt
     4621 cagtggatgc agaaagaatc aaggcggtcc ctgccgctcc cgaacaaatt gcattggcgc
     4681 acaagccgca gttggtcaaa gccattgcag aggtctgcac acgcgggccg ggcatcattg
     4741 accatgcgcc gaccttcatc acgcagacct cgtacgaaga tgccttgctg gccgcgggag
     4801 gtgtggtcac ttgcacgcgc gcagtgctca acggcgaggc gcaaaatgcc tttgccattg
     4861 tccgcccgcc ggggcatcat gccgaacccg accgcgcaat gggtttttgc attttcaaca
     4921 acatcgccat cgcggcacgt gatgcgctgg caaacggcct gcaacgtgtg atggtcattg
     4981 attacgacgc gcatcacggc aatggcacgc aggcagtgtg tgtggatgat gaccgttttg
     5041 gattcatttc gactcatcag tggggaattt acccgggcac cggctggatc aacgacgcgc
     5101 ctcatgcgaa gggacggctg gtcaatgtac ccttgcatga gtttgcaggc gacggaacgt
     5161 tcgcgcgcgt ggctgacgag attttcaagc ccatcgtgga atccttccgc ccgcagatgc
     5221 tgttgatttc ggcgggcttc gacgcgcatt ggaatgaccc gatcacttcg ctgggacttt
     5281 ccacccgcgg gtattacgag atctcgaaaa aacttgttgc gcttgcaaat gaacattgcg
     5341 gcggcaaaat cgtcttcgtt ttggagggcg ggtacgatcc gcacaatgtg gccaacgggg
     5401 cgatggcggt cttcgacgca ttgaccaact ctccgttgaa taaagatgcg ggcgatccgt
     5461 cgccttatcc cgattcagat cacgagtctc gcatccacga tattttgaaa tggcatggca
     5521 tcgcacagta aaaaggtttt gttgtagaat cgcttccgta aaaaggagcg agagatgcaa
     5581 agaaaacaaa gcaaaggttt tgttgagctg gaatgggagt gcccaaactg ccacagcgcc
     5641 aacaaaggtt cgaagaaaat ctgtgaaagc tgcggcgcac cccaacccga agatgtaaag
     5701 ttccaacggg cagcggatga gaagatcatc accgatgaga aggtggtcag tgcggccagt
     5761 gcaggcgcag acgttcattg cggattctgc ggtacgcgca accctgccac ggcaaccaat
     5821 tgttcccaat gcggtgcgga cctaaaagaa ggcaaggcca ggcaggcggg acaggttttg
     5881 caagcggcgc ctgaatcagc gaagatcatt acatgcaaaa gctgcggcac ggaaaacccc
     5941 ggcggggaac gtctgtgcaa taaatgcggt gcgcctttgc cccgtgctga gtctgttgcc
     6001 gccaagcctg cagcagccgt tgcagcgaat tcaggtacca gttcggctgc tgcaccgaag
     6061 gctcaaccga aaaagacgaa ttggcttttg tttggcgcca ttggcgcgtt tttgatggtg
     6121 tgctgcatcg cgatctttgc gatgtttgtg attccctcca agtcggtgca gggaacggtg
     6181 gccgatgtgt attggcagac ttccgtgccg ctgcaggagg ttcgcgcggt ggattattcc
     6241 aatgagcgcg gaaacccgcc agcaggcgca tacaatgttt cctgtcacac tgaaagccag
     6301 gaaatctgcg aagacaagac cgtggatcga ggaaatggtt tcgcagaggt cattacagaa
     6361 tgccacaccg agagtcagga ttattgcagt tacaccgtgg atgagtggac gaccattcaa
     6421 tcgtacgatc taagcggcag tgacttgttc ccagagtatg cccagcccag tgtattcaat
     6481 gggcagcggt tgggcaatgc ttcggcgacg ttgacggtca cttttgatac gccgaaggga
     6541 accatcacct acaccccagg cacggtcagt gagtttcaac agttccagat cggtagtacg
     6601 tggaccttga aactaaatgc cctgggcggg gtcttgagtg taggaaaata aggggcgaaa
     6661 aatgacagac cgggccgaag actcgctgaa aaatgatgtt ttgtacacag atgaccttgc
     6721 gctcaatcgc aatggacagt tatctgaaac gcaaaagaaa cgtctgtggc ccggtgctct
     6781 gttctggctt atgcttgcag gcatcaatgt aatattcctg ctgctcctga gtttctttca
     6841 attgcgctat caaactgtag gttgcgggta tctggggtta ttcgtgatcg cgatcttctc
     6901 cataaggcaa tgcatattaa attccgtgcc atacagggaa gatattaaga atgggatcgt
     6961 aaaaacggct tcagggcagg cattcaagcg ggctgttctt caaagcttca gcaaaggggt
     7021 ttatttgggt aactgttctg tgagagtgaa caacaaggta tttacgatct cgccgcattt
     7081 ttacaactgg atggttgacg agggttatta ccgaatatat ttcactcccg aaacaaaaag
     7141 agtggttaat atcgaagagt tgtaaagtta ccaaaacggc atggacacgt ttcattaaaa
     7201 ctcccgtctc attcgggagt ttttgtcttt ataatcaact tatgaatcat tcagtggcaa
     7261 tcatcggtgg cgggcctgcg gggttaatgg cggcggaagt tttgagcgga catggcatgc
     7321 aggtggatgt gtacgattcc atgccttcgc tcgggcggaa attgttgatg gcgggcaaaa
     7381 gcggactgaa tcttacccat tctgagccgt tcgaaaaatt cgttttacgc tatggcagta
     7441 aaaaacagga agtggaaacc tggttgaacg atttcacacc cgatgacctg cgcgaatggg
     7501 cacgcgggct tggggtggaa acgttcgtcg gcacttcggg gcgggttttt ccaaaagaga
     7561 tgaaggccag tccgctgctg cgggcatggt taaaacgatt aaccgcggcg ggaatcaatt
     7621 ttcatttgcg gcacaggtgg aatggagcaa tcgccgccgg ggagggcggc gggagcattg
     7681 ttaaatttga aacgcccgac ggaatcaaaa cggtccatgc caatgcggtg attctggcgc
     7741 ttggcggcgg cagttgggca agactcggct cggatggcgc atgggttgaa tggctcgatc
     7801 aggccggggt taaggtggaa gcgttgaaac catccaattg tggattcgat gtggcgtgga
     7861 gtctgcattt tcgggagaag ttcgatggtc agccgttgaa atcggtgacc gtttcatttg
     7921 gtgattttca ccagcagggc gaattcattg tgaccaggga gggtgtggag ggaagtttga
     7981 tctacgcggc ttcggcgctg ctacgcgatg agatcgcaca gaatggtcgc gcgatactgg
     8041 aattggatat gtcgccagat cgtccgcttg aatggctggt cgaaagatta tccaaaccgc
     8101 gtggttcgcg ttcgatgtcg agtcatcttg aaaagacagt gggattaaaa ggcgtgaagg
     8161 cgggtttgct gtacgaattt atttcgaagg atgattttgc aaacacagag cggttggcat
     8221 tttttatcaa acacctgccc atcccgctga tcgcaaccca tccactggat gaggcgatca
     8281 gttccgctgg cggtgtgagc tttaaagcgg tggatgaaaa tttgatgttg cacaaactgc
     8341 caggtgtttt ttgtgctggc gaaatgctcg attgggaagc accaacaggc ggatatttat
     8401 tgaccgcctg tttcgcaagc ggacgggttg cagggttggg cgcgttgaag tggagtaacg
     8461 cgggataata tcccgcgcta cggaacccca tgaagaatct tgattaccgc gactattacc
     8521 gtagaaatct tccacacttt cagccgcgtg gcgcaacctt catcgtcaat tttcggctgg
     8581 ctgggtcctt gcctgttgaa gttgtgaaaa tgctaaaagc agaatcagag gaaattgaaa
     8641 agtcgctgtt gagaatcgaa gatcatacag agaggtatca cgtcaaagac agggaacagc
     8701 tcaggctatt ttcgaaatgg gatgaagcgc tccacaacag tcaagccggg ccattttggt
     8761 tgaaagatga gcgcattgct caaatcgtgg tgaactcgat tctgtatcac gacgaaaact
     8821 ggttcgacgt gctggcgtat tgcatcatgc cgaatcatgt tcatttgata ttggcgcccc
     8881 gcgaaaatgc cgacgccgat ttcagccttg cgaaaatcat gcacaacatc aaaagaaact
     8941 ccgccaacca tgccaacaag attttagaaa gaacaggcgc attttggcag catgaaagtt
     9001 atgatcgctt tgcccgcaac gaagccgagc ttgagagaat gatcaaatat gtgctgtaca
     9061 accctgtcaa ggcagggctg gtggatgatt ggaaaaaatg gaaatggtcg tattgcaaat
     9121 ttgaaatgta gtgcgagata atatctcgca ctacattaaa agggatccaa ccatgcatga
     9181 agacatattg aaaatacaac aattgatcgc acgctttgcc aatagtttcg atgtgaaaga
     9241 ttgggatggc ttacaggcct gtttcaccga atcgctctac accgattact ctgacttgcg
     9301 cggcacaccg cctgagacca tttcagccag tgagtatgtc aggctccgcc gtgagtcact
     9361 cgatcatttg aaactgcacc atttggtcag caactacgag atcaccttcc caggtttcga
     9421 tgttgccgcc tgccgcgcct ccatggtggt ttggagaaga tctgaggcgg aagatttcac
     9481 gacccactgc atatacgact tccaacttgc aaggcagcgg ggaacatgga agatcagcgg
     9541 catcacgcaa aagattttgt ggaatgatgg aaatgcatcc attcataaag gagccaaaaa
     9601 ctagccatgt atcgaaaagg ctggcgatat tatcgcgacg caaccctgct ttggatattg
     9661 ttctttgtcg ttcttatgat cggcggctac gccctggaac acccgctgta ttggattctg
     9721 gctttgttcg tatgggtggt ccgctcggcg tttgggaatg cctttcaatt tgcgcaccca
     9781 tcccgtcgct tcaactggga cagcagatcc aacctggtgt tcaaggaagt cacgttcaaa
     9841 tcacgtgatg gtctcaccct attcgggcgt tttcatgctg gcagaactcg cgccaccatt
     9901 atcttgatcc acgggttggg tggtgcaggt accgatatgc tggtccatgc ctcgtttctg
     9961 gtcgatgcag gctacggcgt gttcatgatc gatctgcgcg ctcacggcag cagcgacggt
    10021 gacacatcca cctatgggct gcgtgaagca gatgatgttg caggcgcggt ggattacctg
    10081 ctcagccgca tggacgtcaa cggggcaaag atcggtgtgc tgggaatatc actcggcgca
    10141 caagccgccc tgcgtggggc gctgaaaacg gagaatattc gcgccctcgt tttggatggt
    10201 ctcagccctg cccgcctatc tgaccacggc ggcagcccca aatccataca acgctggctc
    10261 aactatcctt ttaactggct gtattatcgg atttatgaat acatgatcgg cggcagggat
    10321 gcgggtgttc tggaggtgat cggacagatc gcgcctcgcc ccatcctgtt aattgccagc
    10381 ggtttaagag acatttactt cagccgatta ttttacgaag ccgccaaaga cccccgcgcg
    10441 ttatgggaaa ttcctgatgg cattcacggt gtggcgcttg ctggcaatcc ccaggaatac
    10501 attgaccgtg tggtcggctt tttcaaccag accctgctat gacggcctgc cctgaatgtg
    10561 gcgcaacgaa ctgtgaatcg cggttccatg aattccttgc cttggaattc agcaacgccg
    10621 cctacggcaa tgtccaccac ctgaccgtgg cggcttacat gctccaacat tccagcaaat
    10681 taacccgcga aggctggcgt tatgagcgtg agctactgag agcatttttg gtggaaaata
    10741 aaacgcccgc ctttatccgc aggcaaaaca gggattgggt ggatggcggc aaacgaactt
    10801 tcaagatcaa atcaaaagac ggatccccat ttatcaatag atccgcgtgg acaaagacga
    10861 ttctcaatgt ccgcgccgaa aatgcggaag tgtattgcgc agatataacc acatgggcgg
    10921 gtgcggtctt gaaagatgca gaagaaataa aactggaaat atgaggacat ccaagacgaa
    10981 cggctcattc cacggccaca aagccatcag gaatcctttg tgacttcgca gcgattaaag
    11041 ctcgttctgt tagccgctcc ctcataatat tatctcgctc cacatcccta tgataaactt
    11101 accaaccatg aaacgcgcgc tacaaacttc attcatcatc tggacgattc tttcggcatt
    11161 ggttctcgcc agtgtgattg tcatctacct ccgcaacggc gaaacgggac tcaatctctt
    11221 catccagcaa tggccgcttt ccaatttgac cgtgacgctg gaagccaccg cctacacctg
    11281 gttgtgcgca ggcgcgatcc tttacctgct ggcgaatgac aaagttaacg cggaaaatgc
    11341 ctttttcgtg gcgggatttt tcatcgtcat tttgctctac ctgaatgtgt tgcgcgaacg
    11401 cttccgctat ggcgattatc aatactatct cgaagccgcc accgcgttga tgaataacca
    11461 acccctgcca gacacttatc tttacctgcc gctttgggcc accctgttgc aattcatcgt
    11521 tccgcttggc gaccagggca tgctggcgat cttgtggacg gtcaacatca tcatgctggg
    11581 ttccttctac attttgctcg tgcgggtcct tgaacactac ggatattcca accgtttcgc
    11641 ggtgatcatt gccgttttgt tcatgtccat taatacgccg ctgcatcgaa gcctgggata
    11701 tgtgcaggtc aatttgatcg ccatggtgtt gatcatggca agcctgctca ctttccgtaa
    11761 aaatgacctc gtctccgcgc tgctgctggc gctagcagtc cacctcaaaa cctcacccgc
    11821 cgcgcttgcg cttgccttcc tgctcgagcg caactggaaa tggctggcat ggttcgcact
    11881 gaccttcatc ctgattgcgc tcatccctgt tgcacttgaa ggcacacgcc cctacttcga
    11941 ttacctgacc aacatcaaca tcctcaccca catcaccgac accaacttcc acgacacttc
    12001 gttcgattca tttttccgat tcttcaaccc cttccttggg atcaacctcg aagccacacg
    12061 cctgatcaca atgggtgcga aggcgctgct tgggctggcg gccatctacg tcatggcgca
    12121 aaatatccgc agccacacct tcgtcagccg cgaaacgcaa aatcatattc tgctcaacgc
    12181 catcccatcg ttgttcatcg tcatgaccct cacctccccc atcgtttggg atcatcacgg
    12241 catcttcacc acccttgcat ttttgctcct gcttcattgc atcgaaaccc ctgcactgtg
    12301 gatgtggttc gggtttgcct acttcctcga attcatcctg ccttccttcg atttcttccc
    12361 ctggtcattt ggccgcctga tcgcaccgct gatcgtcctc tgcctgatgt ggtatgtcac
    12421 acgcagcaaa caaagcccat ccctccttgt ttcggcaaat cgctggtttg agaatctgaa
    12481 attccaaatc tcccaatgaa acaacaacgc ccaattctga ttctgcttgc cgtgagcctg
    12541 ctcattggca ttttcacctt gaacggctat ggcgatagtt gggacgatct ctcacttcgc
    12601 aaatacgctg ccaaatcgct tgaagcctac ggcacatggg tcgcacgcgg cgaagtacag
    12661 atcaccacag aagacctcgg cagttatggc ccatcctatg tgatggcagt ggccctgctt
    12721 tcgcaggaca atcccgatct ccgtcacttc atttattttg tcacatttct tgcaggtgcc
    12781 tgggcgtttt acatgctcgg cttgcgctgg ctctcgcagc cagccgccct cggcgcaacc
    12841 ctgctttacc tcacccaacc cgttctctgg gggcacgcct ttttcaaccc caaagacacc
    12901 cccttccttt ccctctttct tctttcaatt tattttggat taaaactttt cgacgaatcg
    12961 cccaacccga ttcagggatc ggaccgaaga accgcgcttc tcaccgccct gtggcttggt
    13021 tctgttttct cgctctttct cctcaccgat acatttcaca ccgccattgt gaatcttgtc
    13081 cacgctgcaa aagcgggcga aaccaacatc atctccctca ccgcatcaga tatccgaact
    13141 gccgcgcctg aactctacat ccaaaaatat ttcaccctct tcctccgact ccgcgccgtt
    13201 ttctttcttc tgtcttcttt cttcttttta tttcacaagt cttttcgctc tcttcatcct
    13261 tctgccttca cgcgtagcgt ttatccttcg gtcttcctcg gtttcacttc ctccctgcgt
    13321 gttctcggcc cctttgcggg gttgatcgtg gcatactacg cactccgcaa gcacggacga
    13381 aaatccattc ccactctcat cgcctacgct gtgaccgcca tcatcgtcat gtatctcacc
    13441 tggccctacc tgtggacaaa tccgctcggc aatttcatcg ccagcatcaa aaccatgtcg
    13501 ctctacccct ggagcggaca ggtgcttttc aacggccagc agttcgcatc cactgaatta
    13561 cctgcgtcct atctgcctgt gttgctcggc cttcagttga ccgaaccgct ctgggcgctg
    13621 actctgatcg gggtggtcgt ggcaggcatc agattaagag agaagcgtga actgatcgag
    13681 ttgagcatcc tgtggttaat tcttccgctg ttcgggttca tcgtcatgcg gacggcgttg
    13741 tacgataact tcaggcaggt cttctttatt ttgccgccgc tgttctggct ggcaggggtc
    13801 gcgatcgaac aagtcaaacg ccctgcattt caaatggcct tgatcgtttt ggcagcgctg
    13861 cctgggattg tggatgggat tcgtttgcat ccctacgaat atatttatta caatcgcttc
    13921 atcggcggtg tggatggtgc gcagggccgc tttgaattgg attactgggg cgcttcgtac
    13981 cgcgaggcag cggagtttgt gaacactacc gcgcccgcaa atgcaaccgt atggatcgaa
    14041 ggtccttcac agttgttcga gttgtatgcg cgtgaagacc tgaagttgta ttccacacac
    14101 gaagcagacc gcgccgacca ttacgatttt gcggtggcgc tcacccgcga tcacatggat
    14161 caggcttcat tcccaaacgc ggaagtggtc tacagaatca tgcgcggcag ggctgtgctg
    14221 gctgtggtca aaactccgta acgcaggttg tacgcgttat acgcgtaccg caaacttcag
    14281 tttgcattac cgcattccat tgaaaagaca ttgaaaagag aaaatattat gaacttcctt
    14341 gaattgcttt cacaaaaaac ggtcgtcgcc gatggcgcga tggggacaat gttgcacgcg
    14401 catggcgtgg attttggaaa atgtttcgat gaattgaatc tcaccaaccc tgccgccgtg
    14461 gcggatattc accgcgagta tatcgaagca ggtgcagaat tgatcatcac caacaccttt
    14521 ggagcgaacc gctacaaatt gaaaaaaaac ggattggcag acaaggtaaa agagatcaac
    14581 catgcaggcg tggaactggc aaagaggatc gtcgctgctt cgttcaagga agtgctgatc
    14641 gcaggggatg ttggtccgct tggggtgagg atcgctccat atggcagggt caagcctgag
    14701 gaggcgcgtg aagcgttcgc ggagcagatc cgcgcgttgg cacaggcggg cgttgacctg
    14761 atcgtgatcg aaacgatgag cgatctttat gaaattcaag aagcgatcaa agccgcgaaa
    14821 caggcatgta ctttgcctgt ggtggcttcg gtgacgttca cccgcgatga ccgcaccctg
    14881 cttggcgatg atccgatgaa ggtggcgcgc gcattgcacg gggcgggcgc ggatgtgatc
    14941 ggggtgaatt gttcgggcgg gccttctcaa ctgctgcgga ttctcaaaca gatgcggcag
    15001 gctgtaaccg accgctccgc aaagttttgg gtgaagccaa acgcaggctg gcccgaacag
    15061 gtgggcggca gaattatgta ccccgctgat gccgattact ttggcgatta cgcgctggct
    15121 ttccgtgaag caggcgcgaa cattgtgggc ggttgctgcg gcaccacccc gcagcacatc
    15181 gccgcgatga aaaaagcatt tgcaacaaca cctgttcaaa tctcggaaat gtgggaagcc
    15241 tctgaggttt tggaaacaga atctcaagat gtggaacaac caacccaatt ggcacaaaaa
    15301 cttgccgctg ggaaattttc gatctgcgtg gagatggacc cgccgcgtgg actctcgact
    15361 cacaaattgc tggcgggggc gtccctgctg tacgatgcag gcgcagacgt gatcaacgtg
    15421 gccgattcgc cgatggcgcg catgcgcatg tcggcatggg cggtgtgcga tcttgtccaa
    15481 cggcagatcg gggtggagac cactctgcac ttccccaccc gtgggcgcaa ccttctgcgc
    15541 gtgcaaggtg acctgctcgc ttctcatgcg atcgggttgc gaaatatctt cgttgtgatg
    15601 ggtgacccga cttcgatcgg ggattatccc gaagcgacgg acaactacga tcttgtcccc
    15661 tcgggcttga tcaaactcat caagcaggga ttcaacgcgg gggtggatca ctcaggcaca
    15721 agcatcggcc agccgaccaa tttctttgtg ggcgcggcgt tgaatctgtg cccgcaggat
    15781 atggataccg aggtcaagaa tctgcatcgc aagatcaaag caggcgccga tttcttcctc
    15841 acccagccga tctaccgcgc cgatgacgga ccgaaattga tcgaggctta cgaagccaaa
    15901 cacggcaaat tgcagcagcc cattctggcg ggcattctgc cgctggtcag cgcaaaacac
    15961 gccaactttt tgcacaacga agtgcctggc attttcatcc ctgaagaagc gcggaagaag
    16021 atcgaagccg cgggcgatgc ggagcgcgga gtcaagatcg gggtggagtt ggctgtcgaa
    16081 ctgatcgagc agatcaaggg ttgggcgaac ggcatttacg tcatgccgca attccatcgc
    16141 tacgacatgg tcgcggagat catcgaaaag attcgctagt accgcattgc agactgaagt
    16201 ctgcggcaca caacaagata tattcttatg tggaaaaaaa ctcttcaaaa aataattctc
    16261 atccttctcg ttttgctcgg cataaaaacg ggcgttcagg gtctggcctc caccatcaac
    16321 atgcccagcc acgatgtttc tgttgcgaag tgggatgaac gcgccgccgc attgaaggat
    16381 gccctgccct tcaaacgcgg atttgtcggc tacatctccg acatagacat ccccaacgtg
    16441 gaatatgacc gccagaacct tgtcggtgaa ttcacattga ctcaattttc gcttgcgccc
    16501 atcgttttga tccagggatc ggacgatgaa gaatggaaca tcctcagcct gagccagcag
    16561 gcatacgaaa tttggagcgc ggaaaatgcc gcacaatatg aagtgatcaa atttggcggc
    16621 aagttttatc ttgcgcacaa ggtgacccca tgaccgccgc actcagcctg ctttccttcg
    16681 ccctgcccat gctgacaggg attttgctca tccatgcctt gtgggcggag aaaaacaatt
    16741 tcttcgattg ggttttaaaa ctctcgctgg gaacaggcat tgggctggga atttcctccc
    16801 tgctgtattt tgtttatctt ctggcttttg cgggcaagcc ctggttttta tttgtcgagg
    16861 ttttgacctt cggtgccgtc atcatcgcaa caagaaaata tctccgtgtc tctgtgtcgc
    16921 tgtggttcaa gcccacccgc gcacaaacat gggcgctcat ccttgcaggt attgtctttt
    16981 tcaccaccgt cgctggcatc ctcaactact cgcgccaacg cgccctcggc gattgggatg
    17041 catggatgat ctacaaccga gccgcacgtt ttctttttcg cgaccaggcc gcgtggcagg
    17101 agtcatttcc gcctgagatg agcgtcatct tccatgccga ttatccgcta ttgcttgcat
    17161 cgaataatgc cgcccgctgg gacattctca acaaagagac tccctacgtg ccgatgttcc
    17221 aaagtgtgtt attcgccttc gccgccttgg gattgatctt cggcgcgctg ggacgattca
    17281 catccgtcgg tcaagccagc ctcggcttga ttctgcttgc gggagttccc ttctaccttt
    17341 tagagggcgg ccgccaaacc gcagaccttc cgctggcgtt ttacttcctt gccacagttg
    17401 tcttcatctt tgcatattac gaagaaaaaa gattctccct gctgttcttt gcaggcttta
    17461 ccgccgccct cgcagcatgg acaaagaacg aaggcctgct atttttgctc atcgcgggtg
    17521 cagtgatcct cctcctccgt accgcagact tcagtctgcg catttggcac aaacaggtat
    17581 ttcaatcctt cctccccttc tttgctggcg ccctcctccc gttgtctgtt gcgctgtatt
    17641 tcaaagtggc gctcgcacct gccagtgaat ttctcagcgg cggcctgacc aagatcctgc
    17701 aagacatcgc cgacccagcc cgccacggaa tcatcctcgc gtatttcaaa aacatcttcc
    17761 tcttcagcaa cggctggtat cgtgtcggca tcctgccgat cttgtgcgtt tactttttgg
    17821 tctttcacac ccgcaaggct caagggaaca accccgtctt ccccggcttt gccatattca
    17881 ccctgcaaat tatcgggtat tatgcctttt acctgatctc gccctacgaa ctggattggc
    17941 atctcagttc ctccattgac cgccttttca tccatgtgta tcccaccatt ttgtttgtca
    18001 ccctcgccgc gagtcagacc cctgaaacga tttttgagtc tgatgtctaa aaaaatggat
    18061 cgcagacttt agtctgcgcc ttcttcaagc aaagcggact gaagtccgca ttccgatata
    18121 aaaatttttc acaggtaccc acatgctcct cactattgac gtaggcaaca ccaaccttac
    18181 cctcggtctt tacgaaggcg aacaacttgg cgcacactgg cgccttgcca ccgaccacaa
    18241 ccgcatgccc gatgaatacg gcttgcagtt tttaggcctg ctgcaaaacg ctggcaaaac
    18301 attaaaagac attcatggca tttcactggc gtccgttgtg ccgccgctca cggcgcgtgt
    18361 cattcaagca tgccgcgaat atctcaggca agaaccgctc attgtggatg caggcgtgaa
    18421 aacggggatc aaagtccgtt atgaagaccc gaaagcagtc ggcgcggatc gcatctgtga
    18481 tgcggtggcg gtgatgaaac tcttcggcgg tcccgcctgc gtggtggatt ttggaaccgc
    18541 caccaccttc aacgccgtca ccaaggatgg tcaatatctc ggcggcgcga tcaccgctgg
    18601 ggtcaacctc gccgcagaag gcttgttcac acacgccgcc aaactcccgc gcattgactt
    18661 gcagcgcccg ccttccgtca tcggacgcaa taccgtccac gccatgcaat cggggttact
    18721 gtttgggtat gtgagcatgg tcgagggtat ggtggcaaga ttcaggtccg aacttggaag
    18781 cgatatgaaa gtcatcgcta cgggcggcat ggccgaagtc atcgcaaagg aaacacaggt
    18841 cgtggatcat atcgcgccgt ggctcacact cgacggatta agaatcatct gggaactcaa
    18901 tcaataggaa tcgaatgaaa aaaatatttg ccttgttttt ggggatgtct attttattgt
    18961 ctggatgcgg aactggatta ttcgccgaac caacttcaac gccgaccatg actttcacgc
    19021 ctccccctcc gaccgaagcg gagcgtgcta caccaaccca aacctttacc atcaccccga
    19081 cacgcttcgc gacggcttcg cccagcccca cacccacctg ggtgcatcaa ggccctgaaa
    19141 gcgtggaggt gcccatcctg ctttatcact ggatcgcggt ttcacccgat gaagggacca
    19201 attacaaaag cccgtattat gtgaaacccg aagtcttcga tgcagagatg aaactactgc
    19261 acgactgggg ctacaccacc atcaccaccg aaatgctgat caaagccatt cgcgaaggcg
    19321 ccgatttacc gccgcgcccc gtcatcatca cctttgacga tggtcacatg aacaattaca
    19381 ccaacgcctt ccccatcatg cagaaatacg gcttcactgg tgtgctgtat cttgttgcca
    19441 attatctgga tgccgaagat tacctgaccg tggaccagat caaggaaatg gccgccgcag
    19501 gctgggagat cggcagtcat tctgtgagcc attcagacct gacttcgctc gaacccgaac
    19561 gtcagcgttt tgaggtggtg gattcacgcg ccatgttgga atccaaactg ggggtgcctg
    19621 tcctcaccat tgcctatcca tttggcgtga gcaacagcag tgtgatcgac tatgcccatt
    19681 tcgcaggtta catcggcggc atggggttgg gctacaccca cgatcaaggc ttgaccaatc
    19741 ttttcaattt acaacgccgc gatgtgaagg ggacttatga cgtgaagcaa ttcgcgtcct
    19801 tcctcccctg gcagggcgat gccatctttt tacccaccga cactcccact cccaccatga
    19861 ccccaacgcg cacacccatc ccaacctaca cgtggatcgt gccgagtacg tcaacaccgt
    19921 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtcttcgt ggtgaaaagg
    20041 tgggcatttc aaacactttc tgagggtctt aatttatttc tctccaaacc gcaacaagac
    20101 cacgcccatc aaaaccaacg cgccgccgaa tagctgcatg cctgtcagca cttcattcaa
    20161 aaggaaatat gcccaaatgg cggtgaaggc aggttcaagg gtggcgatca aattggaagt
    20221 ggtcggggaa agataacgga tgctcagggt gtaaagccca aatccgccga gtgtgggggc
    20281 cacgccgagg aagaacaacc atccccaccc gctgaccgaa tccccgagcc agagcaggtc
    20341 tgcaaatgcg cctttgcccg tcaggaacag gtcatttccc aaattgaaga aaaataaaaa
    20401 gacggtcgcc catgcgaaac tgtagagcag ggctgtccat gaattgatgt gggtatctga
    20461 ggcgtgtttg ccttcgaggt tgtaaattgc aaacagcaga cccgagagca gaccgaagat
    20521 aatccccagc ggattcagat tccatgcgga ggggtcgtac gcgcctgaaa caaaaatgat
    20581 gcccgccagg cttagcagaa tggaaacgat cttgatgcgg ctgaactgct ctttcaacac
    20641 aatgcggctc aggatggcgg tcattgccgg ggaggaaaat gccattaccg tggcaacagc
    20701 cgccccgttg tattgaaccg agaaggtcca catcgagttg aagatcgcga gggtcaatcc
    20761 ataccagacc atgaagcccc aatgtttacg gtccagttga aagagcggac ggctgaacac
    20821 cagcaagccg accgccatgc caaacgaaac gaacagatca cgccagaaag ccagcaccag
    20881 cgaaggcagg gaatagtccc tgctcaggta gctgatcaaa atccctgtgg tggaccaaat
    20941 gatcgtggcc gtaagcgcaa tgacgtatcc gcgtgagagt tgtgacgatg atttcatatt
    21001 cgctccaaaa aaagacagcc accttcaaaa agcaggtgac tgtcagaagt gtgcccccac
    21061 gggaattcga atcccggttt aagccttgag agggcttcgt cctaggccgc tagacgatgg
    21121 gggcttgctt ggcgggttgc attgtatcac tgcaaatttt acccgtcaac aagttatgaa
    21181 caaacaatca ttagtataat caatcaacca ttgtctcaac aaaggcatcc actatgacct
    21241 tcccctcttt tctaaaactc acacccgaat atcgcgatta cgtctggggc ggagaaaaac
    21301 tgcgccccgg gcactccccc actgccgaag cgtgggtggt ctatgaaaat gaccgcatcg
    21361 aatcaggcgc gctggcgggc aagacattga gcgaagccgc aaatgaattt ggcgcggcct
    21421 tgctcggcga aaaagttttc gcgcgcacgg gcacacgctt tccgcttttg gtcaaattgc
    21481 tagattgcgc gcaatggctt tcggtgcagg tccaccccaa cgacgagcaa gctgttcaac
    21541 tcgaaggccc cgggcaattt ggaaagaccg aagcctggca tgtgctcgaa gcccaacccg
    21601 attcaaaatt gatcgcaggc attcagcccg gggcaagtgc ggctgaaatt gcagaagcca
    21661 tccgcaacgg cacgatcacc gaatattcac agtatgccag cgtacagcag ggcgatacgg
    21721 tcttcatgcc cgcgggcacg ctccatgcgc ttggtccggg cctgctcatt tacgaagtgc
    21781 agcaaacctc cgattggacg taccgcgtct acgattgggg acgtccgcaa actgaaacgc
    21841 gccgcctgca cattgataaa tccatcgcgg tcacccgtca cgacctcgcc gcgccgatca
    21901 tttcagcacc accctctggc gatgggacac ggcacatcct cacccaatgc gaatacttca
    21961 cgctcgaatt aatttccgca tccacgcgga ccgttgaact ggatacccaa aaagaatcat
    22021 tccacgcgct gacggtgatc gaaggccgcg ccgaacttgt ctctgcaacc gaaacagtga
    22081 ccctcgaaaa attccaaacg gcgctggtcc ctgccagttt gggaagctac cagcttcgcc
    22141 cgttggaaga agaagcgtgc cgtgttttaa aatccagtgt ctgattacct tacactttca
    22201 aaatcgggac gctgacgaac gcagaaaacg cagatttttt ctttttgaat cagcgaaaat
    22261 cagcgttttt ctgcgtccaa aaagttttgt gcaaggtaat cagatccagc gtttaatttt
    22321 tcagtccact tccatcaggg ctgagcgttt ggtcaggtca atttcattca tggccatcac
    22381 tctgaaactg attccctttc cgcaaattaa aaatcacaag cggctgtttt tttgtatcgc
    22441 cccataaaaa ctcaaaggta aacgatcatg acaatcaacg acaaaaccgt aaccatcatc
    22501 ggcgcgggca tggctggtct ttccgctgcg cttgacctgc atcgcgcagg ctggcaggtc
    22561 accgtgctcg aagcgcgaaa ccgcgtcggc ggacgggtgc atactgtacg aagtttttcc
    22621 aacgggttga ccgctgaagg cggcggcgaa tacattgacg aagatcatac ccgcatgatt
    22681 tcgctcgccc gggaattcaa tctatcattg ggcaaagtcg gaagctggca atcacaaaac
    22741 gacgattggg gtgcgttcga aggcaaagct ggctcaatcg cagactccac gctgtggggc
    22801 accaaccttg ccagtgaaat tcacaacatg tggcaggccg ttgcagaact ggggaaggaa
    22861 gtgaacgatc cttccaaccc gcaaactgcc cgccgcgccg aagaactgga tcatcgtccc
    22921 gcatccgagt ggatcgccgc ccaacctgtg catccgctgg cacgcaaggc ctttatccag
    22981 cacatccgct cagaatacac gtgtgaaccc gaagccttct ccctgcttga ccttgcccgt
    23041 aatgcctcct tctattacag tgacgcagaa gcatggccgc agacccaccg catcatcggc
    23101 ggaaacgacc aattgccgca agccatggcc gcccgcctgc ctgatgtgcg gttaaacgca
    23161 gaggtggttt ccatcaacct ccaaccgcaa aaagtaaccg tagcctataa acagggcgag
    23221 gtgtaccaca cgatcaattc agtctttgcc attctggcca ttccgctcac cacagcccgc
    23281 ctgatcgatt tcaaaggatc gctgccatcc gcacatcaaa agatggtgaa tgaaatttcc
    23341 tacggcaaag tcaccaaagt gatgatcgaa tatcgtaacc gcttctggaa ggacaaaaaa
    23401 tggaatggcc gcctgaccac cgacctaccc atcgtcctga catgggatgc caccagtcat
    23461 ctcgaaggcg agcggggcat cctcactgcc tacactggcg gcggcccggg cgcatacctt
    23521 accggcctat ctgacgaaga acgtacgaaa accgccattg cggtcattga aaaaatattc
    23581 cccggatcat cagacctgat cgaaagcacc gccaccattg catggggcaa cgaaccgttc
    23641 agccgatgtt cctacatggc acttgccccc ggcgaggtga ctgcccattg gaaaaccctg
    23701 ttcagccccg ctggccgttt gcactttgca ggtgaacatg caacacccta tcagggatac
    23761 atggaaggcg cagtagaaag cggtcagcgc gccgcgaaac atattatgga gagtgtatga
    23821 gtgaatcaag caaggcattt tgggaaggcg tacgcgccga attcccatta ttgatcggcg
    23881 ttttcccgtt tggcatgatc tatggcgctc tggcgctcaa tgccgggtta tccacgctgg
    23941 catcgcaaat gatgtcgtcc atcatatttg caggctcgtc gcaattcgtc accgcgcaat
    24001 tggtgcatga tgccgcccct ggcctggtca tcattgtgac cattgcagtg gtcaaccttc
    24061 gtcacatgct gtacagcgca tcgcttgcgc catacctgaa aaacctttca ctccaatgga
    24121 aggccctgct ttcctacctg ctgacagatg aagcctacgc gcccagcgtg ttgaaatacg
    24181 aagcggaagg catcaacgcc tcaagccatt ggttcctgtt cggtgcgggg ctttcgcttt
    24241 ggtccacatg gcaggtcagc acagccattg ggatattcct cggcacatcc atccccgaat
    24301 catggtcgct ggattttgca ttgccgttga cctttatcgc gatggttgtc cccgccttga
    24361 aaaaccgccc gatgatcgcg gccgcactat ctgctggcgg agtggcattg ctggcgcaca
    24421 acctgccgtt caaacttggc ctcatcctcg ccgccctgac aggcattgtc attggcaccc
    24481 tgtttgaagg caggcaggac gaaaaggggg cgcaatgaac atctggctgg tcatggtcat
    24541 cggtggtttg atcacatttg gaatgagatt ttccctgatc tatctgtttg gcaggtttga
    24601 aattccagaa acaatgcgca gggctttaca ttatgtcccg cctgctgttc tttctgcgat
    24661 cattttccct gagttgttat atcacgcaaa tcaaattgat ttttcattcg gcaacatacg
    24721 cctgcttgca ggcattgttg cgactctggt agcctggttc acaaagaaca ctctgctcac
    24781 catcctggca gggatgctgg ccctgttcct ttttcagttg ctgtaactgt tttagggaaa
    24841 taaaaagccc cgcggttgct tttcttcagc aaccgtgggg ctttttcaat tcacttcaca
    24901 gcacctacat atagatcaaa aacacctggt cgccgcccgc ctctttggca cgcttcatag
    24961 cctgcaatgc ctgctggatt aagggctcca cctccaccga ggtgctgata cgcgccaccg
    25021 aagcaattcc agcgctgacc ttgatattca gcggggtgtt gttatgcacc accacccgtg
    25081 aactacgcac ccccgtgata attctttcgg tcaccttttc cgcatccgca ccgatcacgc
    25141 cggagagcat caacacaaac tcatcacccg cccagcggcc aatgcaatca taaggtctgc
    25201 ttttttcgcg gatggcttgc gccacaacat gcaaaacctg gtcgccaata tccctgccca
    25261 gtgaatcgtt gatcaccttg aaatgatcca catcaagggc aattaaactc acaggcatgg
    25321 aagtgcgccg tgcccgttcc aattcaccca tggcctgctt gaaaaaagcc gcccgattca
    25381 aaaaacccgt caggctatca aaaaccgcct gatgctccaa ctgttcacgc gccacggcca
    25441 gattgctcgc aagcgagatg atacgctcag ccatgccaac gcgattcttc aggtccttcg
    25501 ccctgaacgg cctctggaga atatcatctg caccggatgg ggtttggtca tcatcggtga
    25561 tcttcgccat ggtcagcaaa atgtacaaag gctgggtcag tttgatcgcc cgcgcgcgca
    25621 aaataaactg cgaacgccgc acgtcgcttg tatcccagtt tgcaatgaca aaccgcgcct
    25681 cacccgattg aatataaggc caggcttgct cgctggaagc gatcggaatc aggctggtct
    25741 tgccgtcatt aaatgcctgt tgaatgatcg caaattcgtt ggggtcgttt tcaagcacga
    25801 gtattttcat ggagatacct tccgtgattt tatgattata gcaggggagt cgaatgggca
    25861 aatccccaca tggtcacctg ttctggattt aatatgattt tcccggcttc acgttgacaa
    25921 atggcatttc ccatagtaat atttgccccg ctcaacccca aacgccgagg tagctcagtg
    25981 gtagagcagg ggactcataa gcccttggtc gggagt
//
LOCUS       DAOQLC010000060        25543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_634_length_25543_cov_17.653719, whole genome shotgun sequence.
ACCESSION   DAOQLC010000060 DAOQLC010000000
VERSION     DAOQLC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 25543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25543
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_634_length_25543_cov_17.653719"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            517..1008
                     /locus_tag="PL000_11825"
     CDS             517..1008
                     /locus_tag="PL000_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89634.1"
                     /translation="MKKLLIAVLFSTSALATPVVVERFTFPYAGQYTAISEGQEFTAW
                     CAEQSQYLHFGETIDYTLIDGTLAWGVEKEDRLDRLLSLPKPTNVTESYAIQQDIWTI
                     LVGGTGLIDVDNTPVTVHAYQLHNDTRQDLLITFPIDEPLPLPLALTGLALIGLGVRR
                     KNS"
     gene            1005..2921
                     /locus_tag="PL000_11830"
     CDS             1005..2921
                     /locus_tag="PL000_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89635.1"
                     /translation="MNHAIFDTEIIGTGEPVFLVCVRILETGEKYALWAHKSGHLNKL
                     VKLLNRDDLTWVSFNGTKFDCPLIAAWVSGRDEYEIKELATRIIKEGLMPWDAYRLLG
                     VQGLPIDHIDLIEVAPGVMINLKTYSARLHYPNIVDLPFHHDQDLTNEECRVLEKYCM
                     NDLGVTASLFQLLRPRIELRERMGETYGMDLRSKSDAQIGEAVLKKLAGIRKSDNFPS
                     KVQYVAPLIVKPKHPDLINLVYLLEQEVFAINPANGSPIEPEWMKQPFPFRNGVYKIG
                     LGGLHSQHDIDVCYEADDEWMISDIDAASYYPSIILKCGIVPKLGGNKGELFLAAYEE
                     IYHQRLAAKSNGDKTVADSLKIVLNGSFGKLGSRFCSFYSPDLLLAVTITGQLNLLCL
                     IDDLAKQRGIEVISANTDGIMVRYKKTMRDKMLKTVAAQGKRTGFEYEETQYSRVAIK
                     DVNNYIAIKLDGKIKAKGLYAPAGVLEMKNPTAEICSIAAAEYLRSGTLPEVTIAAQT
                     NIEPFLTVRTINTPGGGIQHTNKVLTDDWWELTPGVWENADGKTVKRKSRPPSYEVYT
                     GGVPFGRVARWYYTTKDLPPITQVNNGNQVAKSEGAKLCLTLPSKLPPDLNKNWYIKE
                     AYEMLKDMGVTVKI"
     gene            3134..3715
                     /locus_tag="PL000_11835"
     CDS             3134..3715
                     /locus_tag="PL000_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89636.1"
                     /translation="MCYAQIKAEKKIEFKHLKTIGLLDEQEEPICMYTHEDLRKWGEA
                     LNKEMPHSLGEELLRYACNWKEEVEGDTAVSITRLVQECHEASRKAGWWTDLLNGEDL
                     HGKRNVPEMLALIHSEISEALEGYRKGLMDDKLPHRSSIEVELADAMIRLCDLAGGLN
                     LDLGGAVAEKLAYNAIRSDHKPENRRMAGGKSF"
     assembly_gap    3718..3817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3890..4270
                     /locus_tag="PL000_11840"
     CDS             3890..4270
                     /locus_tag="PL000_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89637.1"
                     /translation="MEQTIGELIDEYRKIQLSRADLAKQEKELIEKQKGLREGIFAAF
                     EAQKTTAGRGSLAAASITELDVPKIVDEAAAEKFLKRKKWAHVYSRKFEVNNPAWREA
                     KFMHGADLPGIETVIVRNLSVTKI"
     gene            4595..5392
                     /locus_tag="PL000_11845"
     CDS             4595..5392
                     /locus_tag="PL000_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89638.1"
                     /translation="MATPRKTAVATRNATQVALPAEIEAALKADVAKFQERLAVPTGN
                     KISITQDRKFRNPAGDKYDSVQGVIVDFISKKAYYGGRVYDAENITPPDCFALDFVPH
                     DSLIPSENSPKRQADDCKTCPLNAFKSAPNGKGKACKESYVLALLPPDADATSELMTL
                     ELSATALKSFEKYIRDTARDFGPAYSVVTTFVMDDTVDYPSVRCIDAVRADNALIALA
                     YNRREDAAKLLAVEPDVSGYEEAVAPKAKQRLVAAKPAAQPRRVATR"
     gene            5412..5777
                     /locus_tag="PL000_11850"
     CDS             5412..5777
                     /locus_tag="PL000_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89639.1"
                     /translation="MARQRSIVLTKEEKKSALADRNAEIKAAKQEIKEIGTAIKDAER
                     TLAQAAKATEADIKKLNKALAIAEKKVTALELEKENISSAPTASAAPVPAPASEATVV
                     RRTRRTKAEMEAARAAGLA"
     assembly_gap    5838..5937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5975..6373
                     /locus_tag="PL000_11855"
     CDS             5975..6373
                     /locus_tag="PL000_11855"
                     /inference="COORDINATES: protein motif:HMM:NF040395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6378 domain-containing protein"
                     /protein_id="HNC89640.1"
                     /translation="MSKKKKCKDRQRFLDLCDEALTGIKSQARPTVSLADIKKPPPRT
                     VGDFFEDASMTIKQRGKEYDQPSGERSARAVAIAFNAITKRDLTEAEIWLILQIVKDV
                     RQFTGPYHEDSAIDCIAYATLKAEALACQT"
     gene            6357..6767
                     /locus_tag="PL000_11860"
     CDS             6357..6767
                     /locus_tag="PL000_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89641.1"
                     /translation="MLVKPETRFIQGVHKYLPSSTVLHREKMSNPYRGGTADMYYSAN
                     KADLWLEYKFLPSVPQRGIVDAKRIDLSPLQKGWLAGRYKEGRNVGVVVGCPPGGVIL
                     RDLEWEDGISPEDFRARIQSRPAIAEWIVHQTLS"
     gene            7426..7749
                     /locus_tag="PL000_11865"
     CDS             7426..7749
                     /locus_tag="PL000_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89642.1"
                     /translation="MNTTDEASVARMMQQHADYFFNLTAVKGKEHAEMLLQLADVLIA
                     VDLIVGSSSHESRSLIMPALQTIMVVTFQAVLDNAKVPSEKVTEVLEEACVIAETITR
                     AARRN"
     gene            7750..8679
                     /locus_tag="PL000_11870"
     CDS             7750..8679
                     /locus_tag="PL000_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNC89643.1"
                     /translation="MTDAVETMAYVGQTPWHGLGNRVEDGIDLDDFRREAGLDWEVQK
                     SPVFYEDGVSFKDRFVLNRTSDRYPFAVVSSRYKPVQPKEIFEFFRDLLAQHGMKMHT
                     AGSLMNGGRIWALAQTGDSAFIRGSDRVDSYLLLSTSYDLTLSTLAQFTSVRVVCNNT
                     LQQALEESDGRVTVPHTTDFDQEFIKEALGISKEQWSIFSGALQVLAHIDMDVPKARE
                     VLSKVFDLLEDPTKPDPNRKHVEIIVNTFAAQAFKGSDLAGYTAWGLLNGVTEYIDHW
                     KRARNQGNRLNSAWFGEGAEYKKRTLNELLKLI"
     gene            8834..9154
                     /locus_tag="PL000_11875"
     CDS             8834..9154
                     /locus_tag="PL000_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89644.1"
                     /translation="MFKEGEVVRVIGMDKVLLIKEVDETECLLFDNFNTPCLWWPTYL
                     IRKYEDTGGLLDDGCPSCKEQDDIYYESIEIHGHLAEQNCRCHICGTEWVDIYQLEFR
                     ERIN"
     gene            9154..9669
                     /locus_tag="PL000_11880"
     CDS             9154..9669
                     /locus_tag="PL000_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89645.1"
                     /translation="MGWLFGHYTRKDLIKGILERHHPHIIKYCFVGNNMWTVYENNNQ
                     RFIVLFKLKGCPQQTDRWDNWGYKDVDECMYPCDRTCPLSYLDLCTEPVNENARDWRD
                     AVRRHHADKKRKLKKGDVVKLGSRVFTIEDVYKAGYSVRGTDNVSYFLKNNLVVKLEV
                     LTQGETNVVET"
     gene            9653..9853
                     /locus_tag="PL000_11885"
     CDS             9653..9853
                     /locus_tag="PL000_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89646.1"
                     /translation="MSWKPEIKTSGEDWNTNATRFETEAEAVAAAKDIFTRWMLATDY
                     RAAPSDDPVSYKWDPEKGLVNL"
     assembly_gap    9909..10008
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10267..10443
                     /locus_tag="PL000_11890"
     CDS             10267..10443
                     /locus_tag="PL000_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89647.1"
                     /translation="MFGLQVNVGTVPNPDWRWVHGSDMQIMSFNTQLEAEAEKAKWPS
                     IKAAIFRVREIDDN"
     gene            10433..11308
                     /locus_tag="PL000_11895"
     CDS             10433..11308
                     /locus_tag="PL000_11895"
                     /inference="COORDINATES: protein motif:HMM:NF026093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89648.1"
                     /translation="MTTSPLFKPMLACSKSAHNFFDKLRFPLLVSPKLDGIRATVRDG
                     VVYARSNKPIANVNVQTKFRHLNWYDGELIVGDPTSPTCYRDTCSVVNSRNKPARDVD
                     FFVFDCIAQPSNPYFRRLGDVDPKYRLDHLAVHDLYELKVTEGLFLDRGYEGLILRDH
                     NGKYKMGRSTASEQGMLKLKRFLDAEAEVIGFQERMHNGNEATTNELGRTSRSSHKSG
                     KSGRGDLGALIVRTPEGIEFGIGSGFDDSARQSIWANQDDYLGRLVKYKYFPLGSKDA
                     PRHPVFLGWRDRSDT"
     gene            11485..11859
                     /locus_tag="PL000_11900"
     CDS             11485..11859
                     /locus_tag="PL000_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89649.1"
                     /translation="MSELKDYFMSQIVKNPCKAPPLNTTEYKYAQAMKGCGWMSYEDV
                     AQLVHTTPKSAFEFITEKLCKKFPIEFVQRKNPKGGRPVTFFRWDGPPRARLDEKVEF
                     LKLKKNEFNEIVEALIENLGVV"
     gene            11860..12048
                     /locus_tag="PL000_11905"
     CDS             11860..12048
                     /locus_tag="PL000_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89650.1"
                     /translation="MGDTSEVTEKMSSWKDKYKGLVHYLTHNYNDDTWDSTTREMLAE
                     AYEELFGEKIIWCIEGEE"
     gene            12045..12302
                     /locus_tag="PL000_11910"
     CDS             12045..12302
                     /locus_tag="PL000_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89651.1"
                     /translation="MKLPFYRTREALREYDHDASTRMQALINACSISDRRNAHRLERR
                     ALNKVQDAFYLDVTPMVTKTCSEKLSIDFMRYVVNASNLNE"
     assembly_gap    12555..12654
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12655..20855)
                     /locus_tag="PL000_11915"
     CDS             complement(<12655..20855)
                     /locus_tag="PL000_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89652.1"
                     /translation="MTALGVVPVGMEARGYMPPSPALGLGLEYVRPIPGFSSNESQIM
                     RGIRSAYGGAKAGLQGYGASIADVLGARDTAEDWYANAAANARQAELEGPRVSTLGQI
                     NNLGDVVDYGAGKFGEFLPYMALGGAAGLVGRGLGAVAGLRGAELARAGHLAGAAAFQ
                     PGMAGEQALAMHEDPAARVMSPEERLARSQLTGAAQAYVGAAVPNMMGGQLLTRGEAP
                     TIGRTLGQAVGNSVAGGAGMAAMNVAGQLNKGSYDHGYDFNPGQVGEAFAGGVAGMLP
                     VSGAHGVATHVLDRGVSLPTKVYEEGKGALPDLPDQVKQIWSDVTKYAVEKDLDLGRA
                     FDEHVAPALKDLGVVASAYKATFVRAGEAAKHAVQDELARREVDAPTVRDIADVEALA
                     KNHGEEFSEDMRRTTAGLQRTISPDSPVGKALAVATSDQSVLDVLEKIANDPGSALGA
                     KEQMTPEKLKRLQEGFSNPAALNRYADLMMSLIDEGHPSSEQRAALEEIVTKLREGPK
                     QVEERGKTRQVSPLEPEEVRHLYALGQEVLAAKKAGKDLLSILPKPEADTETKFSGET
                     PRPKENVAYTNVAEAFRTAKVSDKTASEILREVQDATLRGFGPQKTARLLVNRALAKR
                     YGIPEDKVKGGRQNTEYTAYYNALYPALQKAYAELTPESVAQGRGRQRVSPEFTQRVR
                     EHVRDLFETTSFGRQWLSREDRGATEINALADFFTGAAQGKKDHGQAALLRVLTHLHA
                     KFTETEPPGSAKLRARSGRPQIKEPSAVDHLIRNIIDAADIEFGGESKLGNDLSTLRW
                     GQDDKVHTQANQILGLLKPAEGGESRTYKDVEKLLADLHEVKRVDSHRVELKTLAKQL
                     NERWKNAEPGSSERFKLGERVRQIYKLLDENSYEGLKKGWGDQGLLPTDKKSARTFDK
                     LLDMLEQYDTGSGRLARPEHRKGYDGSAKHVAEDQNDIDRVQAQFEQGVNPDHEVVGT
                     EAAGGDEEGEYRTQQEYEHAGTNAERRVPHQFVGTEQFKGGKYDPARIGAPVSRHPSS
                     DYAVSWNPEYNESSALGAHVDATFNNGRVGRRTEVRMQDWAKEMSNTIGGTEGAHLTR
                     AMDALLEHDKERAQNPKISDGSLRYVKARADLAARLRELLPDNPNEVGARFFANPINA
                     HYRYHKIEPHDASNLGYGIRQIENWGGIRNTSVTDWANERSKNSEFSPEVFKDSVLHV
                     RVKDEGGETRVVPIDIAKMVTREMRHHQAGDITGLGDKVKEDNRAQEILTAFSNVIGA
                     ILSTPEVTGIKRGNKFFTADDARTTRESSLKGGNLTLPDAKIGEHFASNLVVYRAEVG
                     EVGQKTTYKDVAGMRPIPGKEGQYFTLGELGHVRDSDRGALVTGTSRVIDGKRVPWSP
                     GGLEGQALETILGKGYQGLPKTTPEGFVRKESSREKRERERDNARGIANGGLEKDGKS
                     YDVDVAALLKTMLVRNGEDPQTVIRDRHLPEDYVGMLLREAFHELKEQGYTGDLLDMA
                     SGKYDNLVPYTKTFGEKTDPVYFSDLKSAILNPAKQPLPHNFEDARNSDIMKRIMASE
                     KAIQDARANPGKDSNASPQLERDREARVERAYRDYDKWAYTDWRDPDVAEPSTFEVSD
                     LTRQANEALAAYRSAKEQLATLDTNSPKYTEQAKKVAEAKAALDLIKRRQDSKDKLFA
                     GSEEGEQFSPHVNPGSHESAAKYGEAIERVNRLEKNAPLFDKSEAVGGDRTNTQRQTR
                     DEVAPIEVTGDKPGVEDHLKQLYEKWMARAKGDPAQEKFIRDTFELPFMDEKGNLSKE
                     FEAYLANKDNRGAFPAEPPVEAKNIPGQPVRKEARPVAKEVSGPEVFPEGIRKGYDRK
                     SLHAPEVFEGEAAAKPEPLKKAEPSAADRVNAMKPDTLARVLNTTDETTDQSTYRFID
                     ALSDQQLHDVAVKLIPDHEDGNRAGQMVTALSDPPSRMREWAESHLKRAGLKYDEAKD
                     RLVSTRLSDTPFSRETPLPKDEGSKDPNDQATLKELKDRARKLLGDGFGVEIASEIKG
                     RTDVSGAFADKLISVLDQARSRMGTLHHEIWHAVESVLKELGPNGEKVLEDVYAHMNT
                     PLMRRWIEEKYNDEGVTGQLDKMSERAAFVFQKLSEGERYVPLVKETKTLFQRLADLV
                     RWAADKLGFHTTTVQERSENFMDYVMRGDFARDIDNGLSVRKGLGEKNGDQLMREAAK
                     ILQPGLEALKVVFEHTSTRIAELKIPEYDKVMQLYVGDEGKGGYMGDKRRMEQAFDND
                     IGEIVKNLSEKELAAFKTTEEYKAFLGRVESYMRNAGVDPNEVRNFYRRLDTFDADKI
                     MKNMDEFIADLVNHGGFKGKDKAGQARTLANQIADRGYYYDKDRALFEGRPDLRDKWA
                     ERDFGASVARIIREAVNLAERSRDRTLPDGQTYQIKNLSSLLEAGDAKADSAGKELMA
                     DTIAAYEGTLAADKLSPGLRKFMGTLLFINNVRILPTAVFSQMLEPLQLALRRGTMHG
                     SLDALWRGIREMPRSADFFDARYTQDKWEKLANQIGSAPSRIIANVMAQMQNGMTLPG
                     KIGHWNDKFFKLNFMDQWNRSMHIEATKHAVEFLKEHADIVRKSKDPKMVERSMRYLN
                     ELGVKEGDIKLTDDGLLQIDKADVGNDRVARAIAQYVNEAMAHPDAGSNPMWMNDPRW
                     ALLAQMKRFTFAHSKFVLDRGLKEWKLGNAFPLVPAVLAMPWMMAADGLRDTLTMTDT
                     SYKNNWSLMDYIDHAWARSGNA"
     gene            complement(20852..23854)
                     /locus_tag="PL000_11920"
     CDS             complement(20852..23854)
                     /locus_tag="PL000_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89653.1"
                     /translation="MPGSMLSTARAPTMGSNLAASASEPGTGRSLGFVGSPVYGNDMV
                     DKGPLKSDGAVPVGAFNDSPQGRVALAQARDRMNGVAVQPPVSATQPILTAKEQLAAQ
                     GHDPNGIYRYDRDGNKVFLAEQRRAYAHTGMFDNGNLWNYADGGKVDPVEELLRRTSQ
                     KYGVSNSAPTPTPTPTPTPAPQPRPLGDVIQQRNETLRKINNYADGGKVEQYEFHGPG
                     GPREDRIPVKFAGANIRVSNGERGMILPAKTASNPEAVDAIEDIIQSTNDGREPRRGL
                     GNGGRYAQGAEPIAPEITVPPDAPQRGLLGRALDTTWDATRAGVGKVFNMSPNPEVQV
                     ATPPVEPPAAPSKPTTFEPPEWIKRSNEAMKWVKTGGGTPESFSAGYKPTGYGTQTTF
                     SAASKVPAVIRVAGPVATALDVANRVKLVGDVAKQGGSGADVATAAAEQGSRFAGGMA
                     GAGLGAGAALTYGAPLIAATGPFAPATAAVLGLGGGAAGYFGGEHLVRGLTAAGRAAL
                     GKGWHYANGGVTPRFENGTTDVTRAILPDHPDALKELRVRTGQGEPSSLPPLAVDPAE
                     VEALRKARGTSAAPVAPVLPSPELSPRAFGPDPERFSVPYVQPVVSSGMPYGFDHIVM
                     DGGAPEFIKQGNAGDAKQTPVSNTLRFTDRSFDPTQQTFAPGTGAATVTGGAKNDGRM
                     MPGVKRNALGTTIVVGPGEYTAKDGTPTSDWTKTAAYEDAIRRNAQDQINLAQTQNER
                     AGRDPMVSRGLGQAMLPPGSDAANDMSSLEAGQRTVAQNLMSPNPGTRALAARQQALL
                     DNMWSKQMAVNKMNRDYAIKDTELATEGNASVIAQNKALEERLMRDPDFQTKEGDKTV
                     PDPVKVGEYLNLLASTVRRAHDEAPPEQKHLWYNGSTKKARSPGELDAAQLSELRKLY
                     DIKRRVDSQAGAWFWNGPNVTGDDLRDYALVPHGEDFEAPRLTKKTGKTFIVPREYLS
                     DAAGARNKFMPALGRQQTTAYLPTGGVQ"
     assembly_gap    24019..24118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(24197..25282)
                     /locus_tag="PL000_11925"
     CDS             complement(24197..25282)
                     /locus_tag="PL000_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89654.1"
                     /translation="MPYTPSDIISDPIGTLPTIPVSSSGIFSPGAAFVIKQNADIINA
                     TWNLAGSKYYDYEAKISSLTDLTTGWLATLSMPHVTAGTVTAPTPTEPSMTIADTAPG
                     AVYADFATQAGNIITTLVSKFSSFQSTWFPSEPTTYGAAEAWVYDAISNTSTSAVPAA
                     IKAAILASADASIVADKTRALADATETWAGKRHALPPGALTYATLQIEQKSLDAQAEA
                     ARSTMIKDFEQTYDKIMSAVKMALQNRATALRAAQDYIVAMVQAEGVGEQITGVAYEA
                     QTRMINAAAGFFSQRITAADLALKLSHSAKSLTLEADKANQAADIKPIEFRLEALLSE
                     IKALATMVTALMNNVRAGGSSSYNVSI"
     gene            complement(25286..>25543)
                     /locus_tag="PL000_11930"
     CDS             complement(25286..>25543)
                     /locus_tag="PL000_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89655.1"
                     /translation="ETDGIVHFIPCNLIEMLYAFVFSRKYGVDPDERYPVDDATAFSA
                     IMAGLFSVQRNIQFRDGEMVDWVVNLGIPYIDSIKTEIYRT"
BASE COUNT         6066 a         6814 c         6348 g         5815 t
ORIGIN      
        1 cgaggcgggg gcagagcgcg gcacgaatga ggcgaacgga gaatgtctat accgcgcgac
       61 cgtgacggcc accagaggag gcgcagaatt ttgaagatat ttggagtaca aaatgattcc
      121 actgtgtgaa atctgctctt cggaaatggt acaaagaaag ggcaagtatg gtcctttctg
      181 gttctgcccc tccacagagc acaagtttac tcttagtgta aaaggggtgc ttcgccagaa
      241 agtgctaacg gagttaagca atctcgatac gaattgctcc ccatacccgt acgcagatct
      301 catagatttt gtaaaaacaa cctttggtcc tgtggaccca cttattgagt tctatgtaga
      361 taacccgtac gatgcgcaag acgacgaaga tcattggatg aatgtgaggc cctactgatg
      421 aaaacataca ctattgagca actcaacaaa cccctcagca gggtcatgac gtaagcggag
      481 atcagtacca gtaaccgtat cttaagaaag tgacttatga aaaaactact catcgcagtt
      541 cttttttcca cctctgcttt ggctacgcca gtcgttgtgg aacgctttac gttcccttat
      601 gcaggacaat acactgcaat ctctgaaggc caggaattca cagcatggtg cgcggagcaa
      661 tcgcagtacc tgcatttcgg ggaaactatt gactacacct tgatcgacgg aacgcttgct
      721 tggggtgtag agaaggaaga tcgtcttgac agactactct ctttgcccaa accaacaaac
      781 gtcactgaaa gctacgccat tcaacaggat atctggacta tcctggttgg tggcacaggc
      841 ctgatagacg tcgacaacac gccggtcact gtgcatgcat accaacttca taacgatact
      901 cgacaagact tgctgattac attccccatc gacgaaccct taccgttgcc attggccctg
      961 accgggttag cgttgatcgg actaggagta aggagaaaaa attcttgaac cacgcaattt
     1021 ttgatacaga aatcatcggc actggtgaac ccgtgtttct tgtttgtgtg cgaatactcg
     1081 agaccggaga aaagtacgca ctttgggcac ataagtccgg gcatttaaac aagctagtca
     1141 agctgctcaa tcgcgacgat ctgacgtggg taagcttcaa cgggaccaag tttgactgcc
     1201 cattgatcgc tgcctgggta tctggacgcg atgagtatga aatcaaagaa ctggccacac
     1261 gaattatcaa agaaggcctt atgccgtggg atgcatatcg gctcctcggt gtgcagggac
     1321 ttcctatcga tcacatagac ctgatcgaag tggcaccggg agtcatgatc aatctcaaaa
     1381 cgtactcggc tcgactacac tatccaaaca ttgttgatct accttttcac cacgaccagg
     1441 atttgacaaa cgaagagtgc agagttctgg aaaagtactg catgaacgac ctcggagtca
     1501 ccgcgtcact atttcaactc ctacgtccga ggatcgaact gcgtgagcgt atgggtgaga
     1561 cttatggaat ggacctacgt agcaagtccg atgcacaaat cggcgaagcg gtgcttaaga
     1621 aattagctgg aatacgaaag agcgacaact ttccgtccaa agtacagtac gtcgctccgt
     1681 taatcgtcaa accgaagcac ccagacttga taaatttagt ctatttgtta gaacaggaag
     1741 tgtttgcgat caatccggca aacgggtccc caattgaacc tgaatggatg aagcagcctt
     1801 ttccattccg caacggtgtg tacaaaatcg gattgggcgg attgcattca cagcacgaca
     1861 ttgatgtatg ttacgaagct gatgatgaat ggatgatttc tgacatagat gcagcgtctt
     1921 attatccgtc gatcattctc aaatgcggca tcgttcccaa actgggtggc aataaaggtg
     1981 aattgtttct tgcagcgtac gaagagattt atcatcaacg cttggctgcg aagtccaacg
     2041 gtgacaagac cgtcgcagac tcgctcaaga tcgttctgaa tggtagtttt ggcaaactgg
     2101 gtagtcgatt ctgctcgttt tattcaccag acctgcttct tgccgtgaca atcactggcc
     2161 aacttaatct gttgtgtctg attgacgacc ttgctaagca acgcggtatc gaggtaatta
     2221 gtgccaacac cgacggtatt atggttcgat acaagaagac catgcgagac aagatgctca
     2281 agacagtggc tgctcagggc aagagaacgg gctttgagta cgaagagacc cagtacagtc
     2341 gtgtggcgat caaagatgta aacaactaca tcgccatcaa actggacgga aagatcaaag
     2401 ccaagggctt gtatgccccc gctggcgtac tcgagatgaa gaaccctaca gcagaaattt
     2461 gctcaatcgc tgccgctgag tatctccgct cgggaacctt gccagaagta acaattgcag
     2521 cccaaacaaa catcgagccc ttccttactg tacggacaat caacactcct ggtggcggca
     2581 tacagcatac gaacaaagta ctcacggatg actggtggga attgacaccg ggtgtatggg
     2641 aaaatgccga tggtaagacg gtcaaacgaa aatctcgtcc accctcatac gaggtttaca
     2701 caggtggtgt accgtttggt cgcgtagctc ggtggtacta cacaacgaaa gacttgccac
     2761 ccattacgca agtgaacaac ggcaatcaag ttgccaaaag cgaaggggcg aagctttgcc
     2821 tgacgctgcc aagcaagctg ccgcctgatt tgaacaagaa ctggtatatc aaggaggcgt
     2881 acgaaatgtt gaaagacatg ggagtgactg tgaagatatg aatacttggc caggaggaat
     2941 tagacgtgct ttgtatcaag aagaacatca aaagtggaat gaagccaact atcctggaac
     3001 acgacagctt tgtagcttat gtgacgaacc aacagggcgt tgcgaagagg actcgatgta
     3061 tttaagcgat ggaggtggac ctgtctgcga acagtgttac gagagtgact gtatgagaga
     3121 gcttatttag aaaatgtgtt atgcacagat caaagcagag aaaaagattg aatttaagca
     3181 tcttaaaacc atcggattgc tcgatgagca agaggaacca atttgtatgt acacacatga
     3241 agacttgagg aagtggggtg aagctctgaa caaagagatg cctcatagtt taggagagga
     3301 gctactgcgt tacgcatgta attggaagga ggaggtagag ggtgatactg cagtttccat
     3361 tacgcgactc gtgcaggaat gtcacgaggc gtcgcgcaaa gctggttggt ggactgattt
     3421 gctcaacggc gaagacctgc atggtaagag aaacgtacca gaaatgctgg ccctgattca
     3481 cagcgaaatt agcgaggcat tggagggata ccgaaaaggg ttgatggatg acaagttgcc
     3541 gcacagatcg tcgattgaag tagagctggc cgatgcaatg atcagacttt gcgacctcgc
     3601 cggtggactc aacctggacc taggcggggc cgttgcagag aagctcgctt ataacgccat
     3661 acgctcagat cacaaacctg aaaatcgacg catggctgga gggaaatctt tctaatgnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga aataaactct cacgtaccct
     3841 tgcgcaatgt cacagattct gtaaaatctt aacttcaatg gaggagcaat tggaacaaac
     3901 aattggtgaa ctgatcgacg agtaccgcaa gattcaactt agtcgagctg atctagcaaa
     3961 gcaagaaaaa gagctgatcg agaagcaaaa aggcttacga gagggcatct tcgcggcctt
     4021 tgaggctcag aaaactacgg cagggagagg gtctcttgct gctgcatcaa taacggagct
     4081 agatgtaccg aagattgttg atgaagccgc cgcagaaaag ttcctcaaac gtaagaaatg
     4141 ggcgcatgtc tactctcgta agttcgaggt aaacaaccct gcatggaggg aggctaaatt
     4201 catgcacggt gcagatttac ctggaatcga aacagttatt gttagaaacc tttcggtgac
     4261 aaagatatga acgacgtgcg cagagtgttt gagggatgga cggctctgaa taagcctgaa
     4321 acaaacctta cccgtgattt gaacacgggc tatgttgatc caacggtgaa tgaaaattgg
     4381 ctcaacttct ccgcaggttg gatattgcga ggcacaaaac tttttcaagg agcatagctc
     4441 aaagagcatg cttcaaagag catgactatc agggactgaa acgctggttt gcggcgttag
     4501 cttaataagc gcttacgaaa gtaaggacac catgccccga agggttgatc tgataggaaa
     4561 acgccgcact cactttttca aggagatcgt taaaatggca acaccgcgta aaaccgcagt
     4621 cgcaacacgt aacgcaacac aggtagcact ccctgcagaa attgaagccg cgttgaaagc
     4681 ggatgtcgcc aagtttcaag agcggctggc cgtgccaact ggcaacaaga tcagcatcac
     4741 ccaggatcgc aaattccgca atcccgcagg ggataagtac gacagcgtac aaggtgtgat
     4801 cgtcgacttc atttcgaaaa aagcatatta cggtggtcgg gtatatgacg cggagaacat
     4861 cactcctccc gattgcttcg ctctggactt cgtgccgcat gactctctca tcccctctga
     4921 gaactctcca aagaggcagg cagacgattg caaaacgtgc cccttgaacg cattcaagag
     4981 cgcacctaac ggaaaaggca aagcttgcaa agaaagctat gtcctggcgc tgcttccgcc
     5041 agatgcagat gcaacaagcg agttgatgac gctcgagctg tcggcgacgg cactcaagtc
     5101 tttcgagaag tacatccgcg acactgctcg agacttcggt ccggcgtaca gcgtggtgac
     5161 tacgttcgtc atggacgaca cggttgatta tccatctgtc aggtgtatcg atgctgttcg
     5221 agcagacaac gcactcatcg ctctggccta taatcgacgc gaagatgcgg cgaagctgct
     5281 cgctgtagaa ccggatgtat ccgggtatga agaagctgta gcaccgaagg caaagcagcg
     5341 cctcgtagca gcaaaacctg cagcacaacc tcggcgggta gctacccgtt aattcactca
     5401 aggagatgta aatggcacgt caacgtagca tcgttttgac caaagaagag aagaagagcg
     5461 ctctcgcgga tcgcaatgcc gagatcaagg ccgcaaagca agagatcaaa gaaattggca
     5521 cggcaatcaa agacgcagag cgcacacttg ctcaggccgc gaaagcaact gaggctgata
     5581 tcaagaagct gaacaaggcc ttggccatcg ccgaaaagaa ggtcactgcg ctcgaactcg
     5641 agaaggaaaa tatctcgagc gcaccgaccg cttctgctgc tccggtccca gctccggctt
     5701 cggaagccac tgttgttcgc cgtactcggc gtaccaaagc cgagatggaa gctgctcgcg
     5761 ccgctggcct tgcgtaatct tggctccatg ccaagtagca cggaaacgtg ctcacgtctt
     5821 cctgggctga cgaaaaannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngta
     5941 tcgactggga cgaagttaga aaggtactgt tgtcatgagt aagaaaaaga agtgcaaaga
     6001 ccgtcaacga tttttggatt tgtgtgacga ggctcttacg ggcatcaaaa gtcaggctcg
     6061 cccgaccgtg tctcttgcgg acatcaaaaa gccccctccc cgcaccgttg gagatttctt
     6121 cgaagacgct tcgatgacaa tcaagcaaag aggtaaagag tatgatcaac cctccggcga
     6181 acgtagcgcg agggctgtag ccatcgcctt caacgcaatc accaaacgcg atctgaccga
     6241 agctgaaatc tggctgatcc tgcagatcgt caaggatgtg cggcaattca cagggccata
     6301 ccacgaggac agcgctatcg attgcatcgc ttacgcaacg cttaaggctg aggctcttgc
     6361 ttgtcaaacc tgaaacccgc ttcattcaag gggtgcataa atacctgcct agcagtacag
     6421 tattgcaccg cgaaaaaatg tccaatccgt atcgaggcgg caccgctgac atgtactact
     6481 cagcaaacaa ggccgacctt tggttggagt acaagttcct accctcagtg ccacaaagag
     6541 ggattgtaga cgctaagcgc atagaccttt ctcctttgca aaagggatgg cttgcggggc
     6601 gctacaagga aggtcggaat gtcggcgtag ttgtcggctg cccgcccggt ggcgtaatcc
     6661 tgcgcgatct ggaatgggag gatggcatct caccagaaga cttcagagcg aggattcaat
     6721 ctcgccccgc tattgccgag tggatcgtgc atcaaactct gagttaggtt acgcgtgcag
     6781 gcacttgcca cagcagcccg gttcgccgga ttgacctacc gcctgttgtc gactacgaca
     6841 atgatcggtt tccttgtagt tggtgtttac cagatggtaa aggagtcccg gcgaacaagg
     6901 taccggtagc gcggctcctc ggggcggaag aaaccttggt aaccgtaatc cattcgcaac
     6961 gatgaatcta tcgggcactc gaaaagtgcc tgagagaagc gtccaagcca cttacgtgtg
     7021 ttgaattgct cgactccccc gacattcggg aattcgctat tgcgaaattt ggcaacgagc
     7081 cacgtaggca tatgggtagg cgatgaacgt tattccgtcc ccgattcttt ctcgagtttg
     7141 acaaatcgca attcgtgttt gctctacact acagaaggaa ttaacgtatg gtccatgaaa
     7201 actcaatcca acactcgaaa gaggacccca gagttatctt cgccactgga ataattgaag
     7261 aacttagggg gctcctactt cacgacaata accggagagg gatacagcgt atccgggttt
     7321 caatcgatct ggaaacccct gatgacgaaa ttcatctcgg ttgggggaag tcgtttccac
     7381 gagcatgata aacaaaactg tacaacctaa ctcacggaga aagaaatgaa cacaacagat
     7441 gaagcgagcg ttgctcgcat gatgcaacaa cacgccgact attttttcaa cctcacagca
     7501 gtcaaaggta aagagcatgc agagatgctg cttcaactgg cagacgtcct catagccgtc
     7561 gacctgattg tagggtcatc atcgcacgaa agtcggtcgc ttataatgcc cgctctacag
     7621 acaataatgg tagttacttt ccaagcagta ctggacaacg caaaggtacc cagcgaaaaa
     7681 gtcactgagg tccttgaaga ggcatgtgta atcgccgaga ctattacccg agcagcgagg
     7741 aggaattaaa tgactgatgc agtagaaaca atggcctacg ttggccaaac gccatggcat
     7801 ggtttgggta accgagtgga ggacggtatc gacctggacg actttcgccg agaagccggg
     7861 ttggattggg aagtgcaaaa gagtcccgta ttctatgaag acggtgtgag tttcaaagat
     7921 cgctttgtcc tcaatcgaac gagtgaccgg tacccatttg ccgtcgtatc cagccgatac
     7981 aaacctgttc aaccgaagga aatcttcgag ttcttccgcg atctgctggc acagcacggc
     8041 atgaagatgc acacggcagg gtcattgatg aacggagggc gtatctgggc tcttgcacaa
     8101 acaggcgatt ctgcattcat caggggtagt gaccgtgtgg acagctacct gcttctcagc
     8161 acatcttacg atctcacgct ctctacactg gcacaattta cctctgtgag agtggtgtgc
     8221 aacaacaccc tgcaacaagc tttagaagag agtgacggaa gggtaacggt tcctcatacc
     8281 acagattttg atcaagagtt catcaaggaa gcgttgggga ttagtaaaga gcagtggtct
     8341 attttttcag gggctctgca ggtgttggct cacatcgaca tggacgtccc caaggcacga
     8401 gaagtgttga gcaaggtatt cgatttgcta gaagacccga caaaacctga tcctaatcgc
     8461 aagcatgtag aaatcattgt caatacgttt gccgcgcaag ctttcaaggg gtctgatctc
     8521 gcaggctaca ccgcatgggg gctgctcaat ggagtaactg aatatatcga tcactggaag
     8581 cgtgcgcgca atcaaggaaa tcgcttgaac agcgcgtggt ttggcgaagg tgcagagtac
     8641 aaaaaacgaa ctttgaatga gttgttgaag ttgatctagt gggacgcccc caggtgcgcg
     8701 tggggtagat tcgaaaaacg gcaccagtga aacccggacg actcccctct gggcgaaatc
     8761 accgttcgaa aggttcgaga taccttagtc ccgtgacctc acgtcacggg catcaattcc
     8821 aaggagaaaa gaaatgttta aagagggcga agtagttaga gtcattggga tggataaggt
     8881 gttactcata aaggaagtgg atgaaactga gtgtttatta tttgataatt tcaacactcc
     8941 ttgtttgtgg tggccgactt acttgattag gaagtatgaa gatacggggg gtttgctcga
     9001 cgatgggtgc ccaagttgta aagagcagga tgatatctat tatgaatcca tagaaataca
     9061 tgggcactta gctgaacaaa attgtcgctg tcatatctgc ggcactgaat gggttgatat
     9121 ttatcaatta gaattccgcg aaaggatcaa ctgatgggct ggttattcgg acattatact
     9181 cgcaaggatt tgatcaaagg tattctggaa agacaccatc cgcacatcat caaatactgc
     9241 ttcgttggca acaacatgtg gacagtatac gaaaataata atcagcgctt tatcgttctg
     9301 ttcaaactga aggggtgtcc gcaacaaacc gaccgttggg ataactgggg atacaaggat
     9361 gttgatgaat gcatgtaccc atgtgatcgc acatgcccgc tctcatatct cgatctttgt
     9421 accgaaccag tgaacgaaaa cgcacgcgat tggcgcgatg cagtacgcag acatcacgca
     9481 gataaaaaga ggaagttgaa aaaaggcgac gttgttaagc taggttcgcg cgtctttacc
     9541 atcgaggatg tttataaggc gggctactca gtcagaggta ctgataacgt atcgtacttt
     9601 ctcaagaaca acctcgtagt gaaactcgaa gtacttactc aaggagaaac aaatgtcgtg
     9661 gaaacctgaa atcaaaacat ctggtgaaga ttggaacacc aatgctacgc gctttgaaac
     9721 agaggcagag gcagtcgccg cagcaaagga catattcaca cgttggatgc tcgcaacgga
     9781 ttatcgggct gcgccgtcag acgatccagt cagttacaag tgggaccctg aaaaaggatt
     9841 ggtcaatctg tgaagcaaga cacgtaccat atgctgtgga gttcagacac gaatagaatc
     9901 attattcann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tcgagagcga
    10021 ggatttgtac gcgaagctct cacagtctcg gatgtaccgg ccaacatgtg catcagagtc
    10081 tctgcgacat tccccgatgt cccggtacgc gatctatatt tggacggggt gaactacgcc
    10141 aacgtgcaca agaatggtcc tccaatcggg cacgaatgcg cagcaccgaa gcaacatggt
    10201 aaatgcgatt tgtgcagaac ctgttgggac aagaacgtga aggctgtttc gtataaggag
    10261 cattgaatgt tcggattaca agtcaacgtc ggcactgtgc ccaatccaga ttggagatgg
    10321 gtgcatgggt cagatatgca aatcatgagc ttcaacaccc aactggaggc cgaagctgag
    10381 aaagcaaagt ggccatcaat taaagctgca atttttcgag tgagggagat cgatgacaac
    10441 tagcccactg ttcaaaccca tgcttgcatg ctcaaagagc gcacataact tcttcgataa
    10501 gctccgcttc cctcttctcg tcagccccaa acttgatgga atacgcgcca cggtgcgcga
    10561 cggcgtggtg tacgcgagaa gcaacaaacc tattgccaac gtcaacgtac aaacaaagtt
    10621 cagacatttg aactggtacg atggagaatt gatcgtcggt gatccaacca gtccaacgtg
    10681 ttatcgagat acatgcagtg tcgtgaactc tcgaaataaa ccagcgcgcg atgtggactt
    10741 ctttgtattt gactgtattg cgcaaccttc aaacccgtat tttcgacgat taggtgatgt
    10801 tgatccaaaa tacaggcttg atcatcttgc agtgcatgac ctctacgaac tgaaggtaac
    10861 agaaggtttg ttcctcgata gaggttacga aggtttgatt cttcgcgatc ataatgggaa
    10921 gtacaaaatg ggtcggagca cagccagcga acaaggcatg ctcaaactca aacgttttct
    10981 tgacgctgaa gctgaagtca ttgggttcca agaaaggatg cacaacggca atgaggccac
    11041 caccaacgaa ctcggaagaa cctcccgtag ctcccataaa tctggaaagt ctggtcgagg
    11101 tgatctcgga gcacttattg tccgtactcc cgaagggatt gagtttggca ttggttcagg
    11161 ctttgatgac agtgccagac aatctatctg ggcaaatcaa gatgactatc tcggtcgact
    11221 tgttaaatac aaatactttc cactaggttc gaaagatgca ccaaggcatc cagtattcct
    11281 tggttggcga gataggagtg acacatgatt tactgccctg gaccgggaga cgaagaaacg
    11341 tggccaccgt attcgggtca tcctcacgac cctcggatgg aggatgattt cgagtggcca
    11401 gaagacgaca ccgatgaaga agacgaagac aatgattagt gaaatgtatg aagcgtacgc
    11461 agaggctggc tgggagagtg agtaatgagc gagcttaaag attatttcat gagccaaatt
    11521 gtcaaaaacc catgcaaagc accgcccctt aatacaactg aatacaagta tgctcaggca
    11581 atgaaggggt gcggatggat gagctacgag gacgttgccc agttggtaca tacaaccccc
    11641 aaatccgcat ttgagtttat tacggagaag ctttgcaaga agtttcctat tgaattcgta
    11701 cagcgcaaaa atccaaaagg tggtcggccc gttacatttt ttcgatggga tggtccaccc
    11761 cgagcacggt tggacgagaa ggtagagttt ttgaaactta aaaagaatga gttcaacgaa
    11821 attgtggaag cattgatcga aaacttgggg gtggtgtaaa tgggagacac atcagaagtt
    11881 actgaaaaaa tgagcagctg gaaagataaa tacaaaggcc ttgtgcacta tttaacgcat
    11941 aattacaatg acgacacatg ggattctacg acgcgcgaaa tgcttgcaga ggcgtacgaa
    12001 gagttgttcg gagaaaaaat tatatggtgt attgagggtg aagaatgaag ctaccttttt
    12061 accgcacacg cgaagcattg agggaatatg accacgatgc cagcactcgc atgcaagcgt
    12121 tgatcaacgc ctgtagtatt tcagatcgcc gtaacgcaca caggcttgaa cgccgtgctt
    12181 tgaacaaagt tcaagatgca ttttacctag atgttacacc aatggtaaca aaaacgtgta
    12241 gtgagaagct cagcatcgat ttcatgaggt atgtggtcaa cgcgagtaat ctaaatgagt
    12301 gaattcgaca cgcctcacaa actgaccgtt cgttctggag ctgaacccta cgggtgcaag
    12361 gacaaggtgt attcgtctgg atactggatg ccgcatcgaa cctacgcacc cgatgggagc
    12421 ttcacgatgg ggtcgaagtg gataccatat cgaatgagca ccgactgcgg gtcgacagac
    12481 atcagcgcaa ctgatccacg gtgcgcagga tgctcatggc aaacaaagga agagaaatga
    12541 ctagaaatga agcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcgttg
    12661 ccgcttctcg cccacgcatg atcgatgtag tccatgaggc tccaattatt cttgtaactg
    12721 gtgtcggtca tcgtcagcgt gtcgcgcaga ccatctgccg ccatcatcca cggcatggcg
    12781 agcactgcag gcaccagcgg gaaagcattc ccgagcttcc attccttcag tccacgatca
    12841 agcacgaact tactgtgagc gaaggtgaac cgcttcatct gcgctaacaa tgcccagcgc
    12901 ggatcgttca tccacattgg gttactgccc gcatccgggt gcgccatcgc ttcgttgacg
    12961 tactgtgcaa tcgcccgcgc cacacggtca ttgcctacat cagccttgtc gatctgcaac
    13021 aggccatcgt ccgtcaactt gatgtcgccc tccttcacac ccagttcgtt caaatagcgc
    13081 atgctgcgct cgaccatctt cgggtccttg ctcttccgaa cgatgtcggc gtgttctttg
    13141 agaaactcaa cggcatgctt ggtcgcttcg atgtgcatcg aacggttcca ctggtccatg
    13201 aagttgagct tgaagaactt atcgttccag tgaccaatct ttccgggcag agtcatgccg
    13261 ttctgcatct gagccattac attggctatg atgcgactcg gcgcactgcc gatctgattg
    13321 gccaacttct cccacttatc ctgtgtgtag cgagcgtcga agaagtccgc agaccttggc
    13381 atctctcgaa taccgcgcca gagtgcgtca agtgaaccat gcatcgttcc acgacgcaac
    13441 gccaactgca gtggctcgag catctgactg aacacagccg tgggcaagat acggacgttg
    13501 ttgatgaaca gtagcgtgcc catgaactta cgcagacccg gtgacagttt atcagcggcc
    13561 aacgtaccct catacgcagc aatggtgtcc gccatgagtt ccttacctgc gctgtctgcc
    13621 ttggcgtctc ccgcttcgag caacgaagac agattcttga tctggtacgt ctgtccgtcc
    13681 ggcaaagtgc ggtcacgact ccgctctgca aggttcaccg cttcgcgaat gattctggct
    13741 acgctcgcac cgaagtcccg ctcagcccac ttgtcccgca agtccggtcg accttcgaag
    13801 agagcacgat ctttgtcgta gtagtaccca cgatctgcga tctggttggc caacgtacga
    13861 gcctgcccgg ctttatcctt tcctttgaac ccaccgtgat ttaccaagtc cgcaatgaac
    13921 tcgtccatgt tcttcataat cttgtctgcg tcgaacgtgt ccaggcgacg gtagaagttc
    13981 cgcacctcat tcgggtcgac accggcattg cgcatgtacg attcaactcg accaaggaaa
    14041 gctttgtact cttccgttgt tttgaacgca gccagttctt tctcggacaa gttcttaact
    14101 atctccccga tgtcgttgtc gaacgcctgt tccatccggc gcttgtcacc catgtagccg
    14161 cccttgcctt catccccgac atagagctgc atcactttgt cgtactcagg aatcttcaac
    14221 tcagcgatgc gcgtcgaagt gtgttcgaag acgaccttga gcgcttccaa ccctggttgc
    14281 agaatcttcg cagcctccct catcaactga tcgccgttct tctctccgag acctttgcgc
    14341 acggacaggc cgttgtcgat gtccctggcg aagtctccac gcatcacata atccatgaag
    14401 ttctccgagc gctcctgtac cgtcgtcgtg tggaaaccaa gcttatctgc agcccacctt
    14461 acaaggtcgg caagtcgctg gaacagcgtc tttgtttcct tgaccagcgg aacgtacctt
    14521 tcaccttccg agagcttctg gaaaacaaac gctgctcgtt ccgacatctt gtcgagttgc
    14581 cctgtcaccc cttcatcgtt gtacttttcc tcgatccagc gacgcatcag cggcgtgttc
    14641 atgtgtgcgt acacatcctc gagaaccttc tcgccattcg gcccgagttc tttgagcacg
    14701 ctttccacag cgtgccatat ctcgtgatga agcgtgccca tccgactccg cgcttggtcg
    14761 agcacagaga tgagcttatc cgcgaatgcg cccgacacgt ccgtacgccc tttgatctcg
    14821 gacgcgatct cgacgccgaa tccgtcaccc agaagtttcc ttgccctatc cttcagctct
    14881 ttcaaagttg cttggtcgtt cgggtccttg ctcccctcat ccttcggcag aggggtttcg
    14941 cgactgaagg gtgtatccga aaggcgcgtc gacacgaggc gatcctttgc ctcatcatac
    15001 ttcagacccg ctcgcttgag atgtgattct gcccactcac gcatgcgtga tggcggatcg
    15061 gacaatgccg taaccatctg ccccgcacga ttaccatctt cgtggtcagg aataagcttc
    15121 accgcaacgt catggagttg ctggtcgctc agagcgtcaa tgaagcgata cgttgattga
    15181 tcggtagttt cgtccgtagt gttgagcacg cgagcaagcg tgtcaggttt catcgcgttg
    15241 accctgtctg ctgcacttgg ctcggctttc tttagcggct caggtttggc ggcggcctct
    15301 ccttcaaaaa cttccggcgc gtgcaacgac ttacggtcgt accctttgcg aataccttct
    15361 gggaatacct cggggccact tacttccttg gcaactggac gagcttcttt ccgaacgggt
    15421 tggccaggaa tgttcttcgc ctctacgggc ggttcagcgg ggaacgcacc tcgattatcc
    15481 ttattggcca aatacgcttc gaactcttta ctgaggttcc ctttctcgtc catgaacgga
    15541 agctcaaacg tgtcacgaat aaacttctcc tgggcagggt cgcccttggc ccgagccatc
    15601 cacttttcgt agagttgttt gagatggtcc tccaccccag gtttgtctcc ggtcacctca
    15661 atgggtgcaa cctcatcacg agtctgtctt tgagtgttcg tacgatcacc cccaacagcc
    15721 tcgctcttgt cgaacaacgg cgcgttcttc tccaaccgat tgactcgctc aatcgcctct
    15781 ccgtacttcg cagcactctc gtgcgagcca gggttgacgt gaggagagaa ctgctcacct
    15841 tcttcgctcc ctgcgaacaa cttatctttg gaatcttggc ggcgcttaat caaatccaaa
    15901 gcagccttgg cttctgcaac ttttttggcc tgctccgtgt acttcgggct gttggtatcc
    15961 aacgtggcca attgctcttt ggcactacga taagctgcaa gcgcctcgtt tgcctgtcgc
    16021 gtaagatcag aaacttcgaa agtcgaaggt tctgcaacgt ccgggtcgcg ccagtcggta
    16081 tacgcccatt tgtcgtagtc cctatatgcg cgctcgacgc gcgcttcgcg atcccgctcg
    16141 agctgcgggg acgcattgct gtcttttcca gggttcgccc tcgcgtcctg aatcgccttc
    16201 tccgaagcca tgatgcgctt catgatgtca ctattacgag catcctcgaa attgtgaggg
    16261 agaggttgct ttgcaggatt gagaatcgct gatttcagat cgctgaaata cactgggtcc
    16321 gtcttctcac cgaacgtctt ggtgtacgga acgaggttgt cgtatttacc acttgccatg
    16381 tcaagcaagt cgcccgtgta tccctgctcc ttcaattcat gaaacgcctc gcgcagcaac
    16441 atgcccacat aatcttccgg caaatgcctg tcgcggataa cagtctgcgg gtcctcgccg
    16501 ttgcgtacca gcattgtctt gagcaacgcc gccacgtcaa cgtcgtagct cttaccgtct
    16561 ttctccaatc caccgttggc gatgccacga gcgttgtcac gctcacgctc acgcttctcc
    16621 cgactggact ctttgcgaac aaaaccttcc ggcgtggtct tcggcaatcc ttgatagccc
    16681 ttaccgagaa tcgtttcgag agcctgcccc tccaaacctc ccggagacca aggcacgcgc
    16741 ttgccgtcga taacgcgact tgtacctgtt accagcgcac ctcggtcgct gtcacgcacg
    16801 tgcccaagtt cacccagagt aaagtactgc ccctctttgc ctggaatggg acgcatgcca
    16861 gcaacatctt tgtacgtagt cttctgccca acctcgccaa cttccgcgcg gtacaccacc
    16921 agattgctgg cgaagtgctc gccgattttt gcgtcaggga gtgtaaggtt tccacccttg
    16981 agtgacgact cgcgtgtcgt acgtgcgtcg tccgcagtga agaacttgtt cccgcgtttg
    17041 atcccagtaa cttcgggggt agaaagaatc gcaccgatca cgttcgaaaa cgcagtcaga
    17101 atctcctgcg cacgattgtc ttctttgacc ttgtctccca gaccagtaat gtcgcccgcc
    17161 tgatgatggc gcatctccct cgtgaccatt ttcgcgatgt cgatgggaac gaccctcgtc
    17221 tctcccccct catctttcac gcgcacgtgg agaacagagt ccttgaacac ttctggcgag
    17281 aactcactgt tctttgagcg ctcgttcgcc caatccgtaa ctgacgtgtt tctgattcct
    17341 ccccagttct ctatctgccg gatgccgtag cccaaattag aagcatcgtg cggctcaatc
    17401 ttgtgatacc ggtaatgcgc gtttatcgga ttggcaaaga agcgcgcacc aacttcattt
    17461 ggattgtccg gcaacaactc acgcaaccgt gctgcgaggt cagcgcgagc cttgacgtag
    17521 cgcagtgacc cgtcgcttat ctttgggttc tgcgcacgct ctttgtcgtg ctcgagcaga
    17581 gcgtccatcg cccgtgtgag gtgcgccccc tctgtaccac caatggtgtt tgacatctcc
    17641 ttcgcccagt cctgcatccg cacttccgtg cgccgcccca cacgcccgtt gttgaatgtc
    17701 gcgtcgacat gcgcaccgag agcagaggac tcgttgtact ccgggttcca gctcacagcg
    17761 tagtcggacg aaggatgccg cgataccggt gcacctattc ttgccgggtc atacttcccg
    17821 cccttgaact gctccgtacc gacgaactga tgaggcacgc gacgctcggc gtttgtcccc
    17881 gcatgttcat actcttgctg agtccggtac tcgccctctt cgtcaccgcc cgctgcctca
    17941 gtgcctacaa cctcatgatc aggattcacc ccttgctcga actgcgcctg cacgcggtcg
    18001 atgtcgttct gatcttcggc gacgtgtttg gcagaaccgt cgtagccttt acgatgctca
    18061 ggacgggcca gtcgaccact gcccgtgtcg tactgctcga gcatatcaag gagcttatca
    18121 aaggtacgtg cgctcttctt atcagttggc aagagacctt gatcccccca acccttcttc
    18181 aacccctcgt agctgttttc gtcaagcaat ttataaattt gcctaaccct ctctccgagt
    18241 ttaaacctct cactcgatcc aggctcagcg ttcttccacc tctcgttaag ttgcttggca
    18301 agcgttttca actctactcg atggctatct actcgcttca cttcgtgcaa gtcagcgagc
    18361 aacttctcca catccttata tgtgcgtgac tctccaccct ctgcaggttt cagcaagcca
    18421 agaatttgat tagcctgcgt gtgtaccttg tcatcttgtc cccaccgcaa cgtgctcaga
    18481 tcattgccca gtttcgactc accaccgaat tcaatatccg ccgcatcgat aatgtttcga
    18541 atcagatgat cgacagcact cggctctttg atctgcgggc ggccagaacg agcgcgtaac
    18601 ttcgcactac ccggtggctc cgtttccgtg aacttcgcgt gcagatgcgt cagaactctc
    18661 aaaagggcag cttgcccgtg gtccttcttg ccttgtgctg ctccagtgaa gaaatctgca
    18721 agcgcattga tctccgtggc tccacgatct tctcgcgaca accactgacg accaaaggag
    18781 gttgtttcga acaaatccct tacatgctca cgcacgcgct gtgtgaactc gggagaaaca
    18841 cgttgcctgc cacgaccttg tgcaacgctc tcaggggtca gttcggcata agctttctgc
    18901 aaagcggggt acaacgcgtt gtaatatgcc gtgtactcgg tgttctgccg accacccttt
    18961 actttatctt ctggaatgcc gtaacgcttg gccagtgctc ggttgaccaa cagccgagcc
    19021 gtcttttgtg gcccaaatcc acgcaacgta gcgtcctgga cttcacgaag aatctccgat
    19081 gcggttttgt cagaaacctt ggcagtgcgg aaagcttccg cgacattggt atacgcaacg
    19141 ttctctttgg gtcgcggcgt ctctccactg aacttcgtct cagtgtccgc ctccggcttc
    19201 ggcaggatgc tgagcaggtc tttgccagct ttcttagcgg ccagaacctc ttgcccgaga
    19261 gcataaaggt gccggacttc ctccggttcg agtggcgata cctgcctcgt cttccccctt
    19321 tcttcaacct gcttcggacc ctcacgaagc ttcgtgacga tctcttccaa tgcagcccgt
    19381 tgctctgacg aaggatggcc ctcgtcaatc aggctcatca tcaagtcagc gtatctgttc
    19441 aatgcggcgg ggttggaaaa accctcttgc agccgcttga gcttttcagg agtcatctgc
    19501 tccttcgccc ccagcgcaga acccggatcg ttggcgattt tttccaacac atccaagaca
    19561 ctttgatcgc tagtcgcaac ggccaacgct tttcccaccg gactatcagg agagatcgtt
    19621 ctctgcaaac ccgccgtcgt gcgccgcatg tcctccgaaa actcttcacc atggttcttg
    19681 gcaagagcct caacatcagc aatgtcgcgc acagtcggag cgtcaacctc gcgccgcgcg
    19741 agttcatcct gtacagcgtg tttcgcagct tcacctgccc gcacaaaggt cgctttgtat
    19801 gctgaggcga cgacaccgag gtccttcagt gcaggtgcga catgttcatc gaacgcccgc
    19861 ccaaggtcca aatccttctc aacggcatac ttcgttacat cactccaaat ctgcttcact
    19921 tggtcaggca gatcgggcaa cgcaccctta ccctcttcat ataccttagt cggcagggac
    19981 acacccctgt ccaacacatg cgtggccaca ccatgcgccc cggaaaccgg caacattccc
    20041 gctacgccgc ctgcaaacgc ttcaccgact tgccctgggt tgaagtcgta accatggtca
    20101 taactaccct tgttcaactg ccctgcaacg ttcatcgcgg ccatgcccgc gccacccgct
    20161 acagagttac ccacagcctg ccccagcgtc ctgccgatgg tcggggcttc gccacgtgtc
    20221 aggagttgcc cacccatcat gttcggcaca gcagctccta cataagcctg cgccgcacca
    20281 gttagctgag aacgcgcgag gcgttcttct ggggacatta ctcgagccgc agggtcttcg
    20341 tgcatcgcca gcgcctgctc gcccgccatt cccggctgga atgccgctgc accagcaagg
    20401 tgaccagctc gagccagctc cgcaccgcgc aacccagcta ccgcaccgag gcctcgtcca
    20461 accagccctg ctgctccacc cagggccatg tagggcagga actcaccgaa cttgcctgcg
    20521 ccgtagtcca ccacgtcacc aaggttgttg atctgaccga gagtgctgac tctcggcccc
    20581 tccagttccg cctgacgagc gtttgccgcc gcgttggcgt accaatcctc ggcagtgtcg
    20641 cgcgcaccga gcacatcggc gatggacgcg ccgtagcctt gcagccccgc cttcgcccca
    20701 ccgtaagcac tgcgaatgcc acgcatgatt tgcgactcgt tggaagagaa tccaggaatc
    20761 ggtcgcacgt attccaaacc aaggccgagg gcgggcgaag ggggcatata cccccgtgct
    20821 tccataccaa cagggactac tccgagtgcg gtcattgaac tcctcctgtt ggcaagtagg
    20881 ctgtcgtttg ctgccttcca agtgcgggca tgaacttatt cctcgcaccc gccgcatcgc
    20941 tgaggtattc cctcggaaca atgaaagttt ttcctgtttt cttcgtcaag cgcggggctt
    21001 cgaaatcttc accatgcggc acaagcgcgt aatcacgcaa atcatcgcca gttacgtttg
    21061 gtccgttcca gaaccacgcc cctgcttgac tatccacacg ccgcttaatg tcatacaact
    21121 tgcgcaattc agaaagctgc gcagcatcca gctctccagg agaacgagcc ttcttggttg
    21181 acccgttgta ccaaaggtgc ttctgttcag gaggtgcttc atcatgcgct ctacgaaccg
    21241 tgctggcaag aaggttaagg tactcaccaa ccttcactgg atcgggaaca gtcttgtcgc
    21301 cttctttggt ttggaagtcg gggtcgcgca taagcctctc ttccaatgcc ttgttctgtg
    21361 caatgacaga tgcgttccct tctgtggcca actctgtgtc cttaatggcg tagtcccgat
    21421 tcatcttgtt cacagccatt tgcttgctcc acatgttgtc aaggagcgcc tgctgacgcg
    21481 ctgccagcgc ccgcgttccg gggttcggac tcatcaagtt ctgcgcaact gtcctctgac
    21541 ctgcttccag actactcatg tcattcgccg catcggaacc cggcggcagc atcgcttgac
    21601 caagccccct cgaaaccatc gggtcacgac cagccctctc gttctgagtc tgagcgaggt
    21661 taatctggtc ctgcgcatta cgacggatcg catcttcata cgcagcggtc ttcgtccagt
    21721 ccgaagtagg cgtaccatcc ttcgcggtgt attctcccgg accaacaaca atagtcgttc
    21781 caagtgcgtt gcgcttaacc cccggcatca ttcgcccatc attcttcgca ccacccgtta
    21841 ccgtggctgc accagttcct ggagcgaaag tctgctgcgt tgggtcgaac gaacggtcgg
    21901 tgaagcgaag ggtattggac actggcgtct gcttcgcatc gccagcgttg ccttgcttga
    21961 tgaactcagg cgcgccacca tccataacaa tgtggtcaaa tccataaggc atgcccgaag
    22021 aaacaactgg ttgtacatat ggaacggaga acctttcagg atctggacca aacgcacgag
    22081 gtgaaagctc tggcgatgga agtaccggcg ctacgggtgc cgcagatgta ccacgagctt
    22141 ttcgaagcgc ttcgacttct gcgggatcaa cagcaagcgg cggaagggac gaaggctcgc
    22201 cctgccccgt acgcactcgc aactctttca acgcgtctgg gtgatcagga agaatcgccc
    22261 gcgttacatc agttgttcca ttttcaaagc ggggagtgac acctccgtta gcataatgcc
    22321 agcctttgcc aagcgctgcg cgaccggctg ctgtgagccc tctaaccaaa tgctcaccac
    22381 caaaataccc ggctgcacct ccacccaatc caagcaccgc cgcagttgcc ggtgcgaacg
    22441 ggccagttgc cgcgattaac ggcgcgccgt aagtaagcgc cgcacctgct cccaagccag
    22501 cccctgccat acctcctgca aatcgcgaac cttgttctgc agcagccgta gcaacatcgg
    22561 cacccgatcc accttgtttt gctacatcac ccaccagctt cactctattg gcaacatcta
    22621 gcgccgtggc aaccggaccc gctacgcgga ttacagccgg gactttcgaa gctgcggaga
    22681 acgtagtttg ggtcccatac ccagttggct tgtacccagc cgagaaactc tcaggagtac
    22741 cccctcccgt tttcacccat ttcatcgctt cgttcgaacg cttgatccac tccggtggtt
    22801 caaatgtagt tggcttagaa ggagctgcag ggggttcaac cggaggagta gctacctgca
    22861 cttcaggatt tggagacatg ttgaatactt tgccaacacc cgcccttgtg gcatcccacg
    22921 tagtgtccaa ggcccttccc aacaatcctc gttgtggagc atctggtggt acagtaattt
    22981 cgggtgcaat cggctctgca ccttgcgcgt atctcccacc attacccaag cctctgcgag
    23041 gctcacgacc gtcgttggta ctttggataa tgtcctcgat agcatcgaca gcttcgggat
    23101 tcgaagcggt cttcgcaggc aggatcatgc ccctctcacc gttgctcaca cggatattcg
    23161 ccccggcaaa cttcactgga atcctatctt cacgaggacc accgggaccg tggaactcat
    23221 actgctcgac cttcccaccg tcagcgtagt tgttgatctt ccgcagtgtc tcattacgct
    23281 gctggattac gtcccccaac ggtcgcggtt gcggcgcagg agttggcgta ggagttggcg
    23341 taggagtcgg cgcagaattc gacactccat acttttgact ggtgcgacga agcagctcct
    23401 cgaccggatc aactttccca ccgtctgcgt agttccacag gttgccattg tcgaacatcc
    23461 ccgtgtgagc gtacgccctg cgctgctcgg caagaaacac cttgttcccg tctctgtcgt
    23521 accgatagat tccgttgggg tcgtgcccct gcgcagcgag ttgctctttg gcagtcaata
    23581 tcggttgagt tgcagaaaca ggtggctgta cagcgacgcc gttcatgcga tctcgtgcct
    23641 gtgccaacgc cacacgacct tgtggtgaat cgttgaatgc accgactgga actgcaccgt
    23701 ccgatttcag cggacctttg tccaccatgt cgttgccata aacaggtgag ccaacaaaac
    23761 ccaacgaacg cccggttcct ggttcgctcg ctgatgcagc aagattcgaa cccatcgtgg
    23821 gagcacgagc ggtagaaagc atggaaccgg gcatgtcgcg aataccactg ggcattgtgg
    23881 ccgtcccagg cattgccacc gacgcagcag ccgactgaga atctccgaat gaactaccat
    23941 tgcgaaaaac atctccaccc gctgcgtagc agttgcccaa cccgcgccga gcggccttcg
    24001 ttttgaatgc ttttgtgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    24121 gtagcagttg cccaacccgc gccgagcggc cttcgttttg aatgcttttg tgatattgct
    24181 ggtcatacgc acctcgtcag atcgacacgt tgtagctact gcttccaccc gcacggacgt
    24241 tgttcatgag cgccgtgacc atcgttgcca gggccttgat ctcagacagc agcgcctcga
    24301 gacggaactc aatgggcttg atgtccgccg cttggttggc tttgtcggcc tctagcgtaa
    24361 gagacttggc actatgggag agcttgagag ccaaatccgc agctgtaatc cgctgggaga
    24421 agaaccccgc cgctgcgtta atcatcctcg tctgagcctc atacgccacc ccggtaatct
    24481 gttctccgac accctctgct tgcaccattg ccacgatgta gtcctgcgcc gctcgcagag
    24541 ctgtggccct gttttgcaac gccattttga ccgctgacat gattttatca taggtctgtt
    24601 cgaaatcctt gatcatggtc gatctggcag cttcagcctg agcgtccagg ctcttctgct
    24661 cgatttggag cgtggcgtag gtcagcgccc ccggtggcaa tgcgtgacgc ttgcccgccc
    24721 aggtctctgt agcgtccgcc aaggccctcg ttttgtcagc gacgatggat gcatcggcag
    24781 aggccaaaat cgcagccttg atcgccgcag gaaccgcact ggtgctggta ttcgaaatgg
    24841 cgtcgtacac ccaggcttcc gccgccccgt aggtcgtcgg ctcactcgga aaccacgtgc
    24901 tctggaagct cgagaacttg ctcaccagag tcgtgatgat gttcccagcc tgagtggcaa
    24961 agtcagcgta caccgctccg ggggccgtgt cagcgatagt catactcggc tctgtcggcg
    25021 taggagcagt caccgtcccc gccgtcacgt gaggcatgga cagcgtagcc aaccagcccg
    25081 tggtaaggtc cgtcagtgag ctgatcttcg cctcgtagtc ataatacttc gacccggcga
    25141 gattccaagt ggcgttgatg atgtcagcat tctgcttgat aacgaacgca gcacccggag
    25201 agaagatacc ggaggaagag acagggatgg ttggtagcgt cccaatcggg tcactgataa
    25261 tatcggatgg ggtgtagggc ataatttaag tcctgtaaat ttcagtcttg atgctatcta
    25321 tatagggtat acctaaatta accacccagt ccaccatttc tccatcacga aactggatgt
    25381 tgcgttgcac gctgaacaag cctgccatga ttgccgaaaa ggcggtagca tcatcaaccg
    25441 ggtatcgctc gtccggatca accccatact tccgactgaa aacgaaagca tagagcatct
    25501 cgatcaggtt gcacgggatg aagtggacaa tgccgtctgt ttc
//
LOCUS       DAOQLC010000061        24988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_658_length_24988_cov_24.504833, whole genome shotgun sequence.
ACCESSION   DAOQLC010000061 DAOQLC010000000
VERSION     DAOQLC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_658_length_24988_cov_24.504833"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="ssrA"
                     /locus_tag="PL000_11935"
     tmRNA           complement(<1..370)
                     /gene="ssrA"
                     /locus_tag="PL000_11935"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            493..1650
                     /locus_tag="PL000_11940"
     CDS             493..1650
                     /locus_tag="PL000_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89656.1"
                     /translation="MAEKILIIDDDVDTLRLVGLMLQRQGYEISAASNGTQGLAKALE
                     ERPNLILLDVMMPDMDGYEVTRRLRKNPVTVSIPILMFTAKTQLDDKVIGFEVGADDY
                     LTKPTHPTELQAHVKVLLARQKDVTEIVTSSKEHVGYVIGVLSARGGLGVSSVAANLA
                     AGIHTRIKADVILAELTPGQGTLGMDLGSPNQKGLTELLEGTTAEVTREKVKSLLVSH
                     NSGLKLMLASENPRDVTLNSKVPNYEAIVAQLSTLARFVILDLGNGLPVHVQKLLPMC
                     TERMVVVEGVPGSIQHTRLLIDEIANLKIDRKTITVVLNNRMRSEAQMQLNQVQELLG
                     HSVSATLTPAPEAFLQATRLQTPVVIGQPSNMTSQQFLKLSDAVLEREKAR"
     gene            1647..2423
                     /locus_tag="PL000_11945"
     CDS             1647..2423
                     /locus_tag="PL000_11945"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNC89657.1"
                     /translation="MTSFSPQTQAAIEFAADLLRQSSHAVVLTGAGLSTPSGIPDFRS
                     TGTGLWAKDEPLEVASLSTFRTAPERFFQWFRPLASQIFNAQPNLAHWALAELEKAGH
                     IDSIITQNIDTLHQKAGSKVVIEMHGTMQTLTCTQCYHQVDAGNYLAAFVETGEIPLC
                     PKCGQVLKPDVILFGEQLPQTAWFKAQRAARQCDLMLVIGSSLEVLPVAGLPMQALDR
                     GAHLVIVNNTQTYLNVRADAVIPEDAAVTIPAIVERVLHG"
     gene            2416..3657
                     /locus_tag="PL000_11950"
     CDS             2416..3657
                     /locus_tag="PL000_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89658.1"
                     /translation="MAEIKTERLDSYRRLIDIARDLASTLDLDILLSRIVHAAAEISG
                     AEAASILLYDDTSRQLYFQVSTNLDESTRRGIVVPLEGSIAGWIVNNRRPVRITNAHE
                     DPRFYSDVEATTGFSTDSLLGIPLVTKNKIVGVLEALNKHRGKFTDSDESMLLVLGAQ
                     AAVAIENARLFQQSDLISEFVHELRTPLSSLSTATYLLLRPEMSQEQRDQIINNIHSE
                     TMRLNALASSFLDLARLESGRVQFRKTTFSVADLIYECKDVMSSKAIEDNIQIRVESP
                     EGLPLLEADREKIKQVLLNLLSNAIKYNRPNGTVMLRAELTEKELVIYVQDTGLGIPD
                     ESLPHLFEKFFRVREHESRIAGTGLGLSICKQIIHGHGGRIEVKSKIGVGTVFSIFMP
                     RTGKGQPLPEDPLDPKKIIKK"
     gene            3794..4375
                     /locus_tag="PL000_11955"
     CDS             3794..4375
                     /locus_tag="PL000_11955"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC89659.1"
                     /translation="MMAQFQFVMRSGPTPGVTFPLEGDQLIIGRDASNSITINDAEIS
                     RKHSRLSFQGGKYVLEDLGSTNGTFVNGQRLAGPVVLKPGDVVSLGEQIVLMYDAINI
                     DTGATIATSRKSARVEPPPAAAVSQPPAPTPTPVYTPAPSYSGPPAVKKTNMLPIFIG
                     GGVLIFVCFCISLLWWIDATYRWCVFFPFLTGC"
     gene            4688..5902
                     /locus_tag="PL000_11960"
     CDS             4688..5902
                     /locus_tag="PL000_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC89660.1"
                     /translation="MTEQQIPSSPVWGTNTKLVVALTVVVIVGALLVKFQFILTPLLI
                     ALVLAYLFHPLSSFLQRKLHLSWGASVGIVYLVVIILLLGLLTLGGVSLVQQAQSLVS
                     VVQTAIRSLPAFIDRISDKIYTFGPFKIDFRAFDLTNLGDQILGMIQPLLSRTGTLIS
                     TVAGGAANFLGWTLFVVLVSYFVLAESGGFRNRMISVDLPGYTHDFERLSRELGRTWN
                     AFLRGQIIIFFIAIIVYTIVLSVLGVHYALSLALMAGLARFVPYVGPAVNWGLMALVS
                     YFQVYKLFGLSPVYYALLVLLIAVIIDQIFDNVVTPRILSDSLKVHPAAVLVATIVAA
                     NLFGLLGVVVAAPILATAAILWKYIMRKMLDLDPWPEEEQTQPTPPPGSRILVSIRRF
                     FRTLSLNRSKGK"
     gene            5991..6167
                     /locus_tag="PL000_11965"
     CDS             5991..6167
                     /locus_tag="PL000_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89661.1"
                     /translation="MSNNEPKTTTIAETENFIAWRAEEPDGETTYHVELNNVTIHFFQ
                     EEWVEFLELVRSLK"
     gene            6240..8297
                     /locus_tag="PL000_11970"
     CDS             6240..8297
                     /locus_tag="PL000_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89662.1"
                     /translation="MKLFQFLLRVGGSLAVALIVSFSSAVIFFNYSGYENLSDRIFLV
                     VVPTLAIGYLFYESFPKYWPWLLQRQPAVLVTFGLLALLAAAVVVLPAAVSNVYYLGL
                     VSLALALFILMLPAAPAVERIRTSHSMLHYMLGFVLNLAVSYGVVGFMGGVFSYRFDV
                     IVFSILITLVGSVIGYYLVRRASRSMRDGFLRNPLNFFLVISLPAFFAAMLFLSLQFP
                     SMFSLAYIQIPLARFGMFISSAVVAGAWSVALLEQVELRGWYAAFKQSKAYAFINENL
                     PGLYAAASFFGINLIIARALNHPTFSINSIVFEADAGPWMSILGFPEGHDVNRAVHPL
                     VLITLRPLVGLIQIFTADKWFLGPIIAVAAMNGLCIFMAWLFAKRATQKDSYAFVFSI
                     ILGVTAAHLLFGSLTETYVFGMTSLILFFVLVQADEKRFSVLVPAGILVFGVTVTNIA
                     QGMIALFFKKFGFWKLVYYGIIVLTISIALTAFVSVLYPGNQTFFWVPADLAFEGRFS
                     KPIYAGPMDRVVEKFGAVGRAIFFHGVVAPTPIEDFTRKTTDPLIDFKTHDYHEHRYA
                     WYNGAAYLPVALWLGLFAGSVLLFFKNLRTSPHIPLMLGLLGSIGFTYFLHMNYGTEL
                     FLYSPYWTYLLIFFLMLAYVDFAGKRWFEAVLTVFAVTLMVNNAWFIFIILRGLAPYL
                     AAG"
     gene            8334..9815
                     /locus_tag="PL000_11975"
     CDS             8334..9815
                     /locus_tag="PL000_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89663.1"
                     /translation="MNQPLRLFLTTLTALAVAFAFSFFFLNLSGRSLAEQGLLIGFLA
                     ASLGWLIWNAPSVLSEWKFAMPKLGLRENLPGLLLALTFFAVYTSIGLQLNTLSVDTT
                     DNFLDADNSSWITRIADPAGSTLEMRGPHPFAYFIFRPFGWLLNLFTGSSALSATLLN
                     TFTGALCVFLVWLFVKRQIQDAVYALLIASLLGLSTAHIFFGSVVETYIFSAAALIGF
                     LLVLQKGSSSLPASVGVSVLTFGITLTNFVQNFILFAAAQIQAGTKNFKTLVLNLFRF
                     TAFTLALGILLSILHAAWFPSSRLFFLPSDAQAEEEFAFSVFQEPSWKVIGRVFLLVR
                     TVFLYAVIAPQPYVFGEEVGGKFPRFNFFRISPETFSYSSYDGVGNVLVLAWAVLLLV
                     SGFFFLRNLVGTRKVDLSAAFILCVLFNFVLHFNYGYEIFLYSPDWAYALILFVALSL
                     APLAKNRFFQGGLAVFLILLAYNQFQFFDFIFKTIALFFDKGA"
     gene            9815..11467
                     /locus_tag="PL000_11980"
     CDS             9815..11467
                     /locus_tag="PL000_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89664.1"
                     /translation="MFQLLNHRMLFAWMLSVITSYFVVGFLGNYYPSFVGVLVLAVIA
                     QTLLGVLIDRLFAKAERLYSSNKGDLFLALALFMVLTVFAAAMLQMANQFPSLFDAKF
                     VLLEQDQTFPFIIAGVLTIPLLALAGQFMGQSRFSAFVDSIATGVLVASFFFAVYFIF
                     SFIFNQPVFDTDDIFFDSDGWLWRTRFTTDAYEDYYWRSLHPFVLLVIRPLVFLVSLF
                     LKGDRLSAGLLLMPLAGAACVFMAWYFVKKVTGNSLYAVLIAALLGGTTGHLIFGFLI
                     ETYIFIAAVAMLSIILLLRDAPLFTLIITGLVSFGITLSNIIQPTIALVFVRRDLKQW
                     IKYGLIVGALAFPLTLLNNVIYPDSQPYFFDPSSYGTEGRNTFEVHPARAVAIGRVMF
                     LNSIVAPDPLILKRELPFLKVWIVEVSRKLYPQEANPMRSSEYETQFGTAMVVFWMAL
                     AALGGFGFLKNIKKQDNRFAYAFIFITLFSFVLHQKFGKELFLYATNWTYAIVLFLAL
                     GWREFMGRRWFQAVMLVFIALMLVNNSRLILTMLSTSALHIR"
     gene            complement(11551..11925)
                     /locus_tag="PL000_11985"
     CDS             complement(11551..11925)
                     /locus_tag="PL000_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC89665.1"
                     /translation="MTKFFFRWAINAVALYVAVLVVPGIDLRGSWTGILWLALIIGLL
                     NALVRPLLKFLTCPLIILTLGLFTIVINTVMLLLTSKIAQSFGIGLTVDGFWTAVLGS
                     LVISIVSIVMSVIFRDELKGKS"
     gene            complement(11960..12964)
                     /locus_tag="PL000_11990"
     CDS             complement(11960..12964)
                     /locus_tag="PL000_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89666.1"
                     /translation="MPSMSSITIPARTETHTNLRPLNVLRDLPAVADLIETCFSSTMD
                     SEGRRYVQDMRRAGNDNSFVKWANRAIETTSLPLSGYIWEENGKVVGNASLVPFKHNK
                     QRIYLIANIAVHPDYRRRGIARALTQRALQHAHEKEINNIWLHVRDDNPGAIELYTKL
                     GFVERARRTSWIASTDPHATSLQTNFIITKRNPRDWPIQHEWLTRLYPEPLAWHRNWS
                     FSPLKPGFWNWLYLAFIDVNIRQWAAAKKETDPQQPAPLEATLAWIPQGHGDALFAAI
                     GERSNPDALTALLLEARCELYHSHPSITLEFPSSQFDTAIQAAGFKAQRTLIWMQATL
                     "
     gene            13025..14287
                     /locus_tag="PL000_11995"
     CDS             13025..14287
                     /locus_tag="PL000_11995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89667.1"
                     /translation="MKKHSLFTVLLSLRGNPRGCVYFEPLWGIPYNLFAPYVSIYMIA
                     LGMSEKQIGLIVSISWGFQIFLALISGVVTDKLGRRFTTLVSDILSWTIPSIIWAVSQ
                     NFWYFLIAGVVNSIWRISHTSWSCLLVEDAEQDQLVDIYTWIYIANLFVGFIAPLAGV
                     LIGVFSFVPTMRGLYVFAAVTFTLKAVLTYLWTEETAHGKVRMQETKDQSVLHVFGEY
                     KGVLRDLVRAPQTLYTAGIMLVMSITSLINGSFWSILVTEKLQIATENLAIFPFVKSA
                     IMLVFYFTVMPAIGRMHFKMPMVVGFLGYVASQVVLVNAPSGGYGFLIFSVFLEACSV
                     AAVSPLIDQMIVLTVDAKERARILSILSVGVILVTSPFGWIAGTLSTLDKDLPFLLNI
                     SLFVIGMVLAYFAGQFSQKKTVMAEAAG"
     gene            complement(14465..14674)
                     /locus_tag="PL000_12000"
     CDS             complement(14465..14674)
                     /locus_tag="PL000_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89668.1"
                     /translation="MDSEFEVQVFDGETKLLRVARLDLLVFNKTFKGEYLLIDRPDGI
                     LGRNILNHIRLLLDGPQQEWGEYKK"
     gene            complement(14840..15067)
                     /locus_tag="PL000_12005"
     CDS             complement(14840..15067)
                     /locus_tag="PL000_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89669.1"
                     /translation="MTLVTYEGVVQKGSILLSEGVQLPENVKVYVIVTEEVISTKING
                     KKPVQIVSPRLAKRDDAARFKMTVTEEKPNA"
     gene            complement(15115..16110)
                     /locus_tag="PL000_12010"
     CDS             complement(15115..16110)
                     /locus_tag="PL000_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89670.1"
                     /translation="MLSPADLIHLPYTPDLTEGGIAYALRTLPNRFDHAEGRQYDQLR
                     RTVAGAAVELAFRRHLSQQGIPFEIASVNPFTNPDQYDVSLGGRRCDLQSFFISHRNQ
                     IAQMRSDPSLILNAPALVPSEHHVNDGRADNDLYLFAFLSGLIAASQDDLKKVIETNQ
                     PRYLIHLMPEAWRRPYQWTPLAPLVLKSESDEELIVEISGQDEGRGFVTRVLSLPPKV
                     RVIVDDSFYSVMAIHVRNIPSARIGIHSPAFKEAHVIAPLEWGNLWLYGMDITLTGWI
                     TREEFRRRAKLIAPNSRVFQYDRTKTKNLAVPVSSLNPLGELFVRVREWEAGRKA"
     gene            complement(16165..17085)
                     /locus_tag="PL000_12015"
     CDS             complement(16165..17085)
                     /locus_tag="PL000_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC89671.1"
                     /translation="MKILFLFLDGIGLGENNPEINPFARANMPYLESLLKGRKLLRES
                     APVDSESAALFSINPRLGVNGAPQSATGQAVLLTGINIPAEIGYHYGPKPNPEVAQYL
                     DGKTLFAKTVQAGKTAALLNAYPPRYFEGIDSGKRLYSAIPLALTNAGLPLFTHEDYF
                     AGKALSADFTGKGWHEFLNFPEAPIFETEEAGKRLADLARQYDFSFFEYWASDYAGHK
                     QDMGSAVRQMERFDSVLKGLLEHWDSNDLVLLTSDHGNMEDLSTRKHTEADVPLLLFG
                     EKSIRDEFGKDIRDLTGIAPAIEKGLGIRN"
     assembly_gap    17200..17299
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17300..17863)
                     /locus_tag="PL000_12020"
     CDS             complement(<17300..17863)
                     /locus_tag="PL000_12020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89672.1"
                     /translation="MNFFRRISFNFWYLRRPPWDSGITPPELFEFIQSHPAGRALDIG
                     CGTGTNVITLAKTGWQVQGFDFASRAIRIAKRKIKTAGIQADLFTDDATQMKNISGQF
                     DLALDIGCFHGIPNKADYLTQLDRILAPNGFWLMYGFFRQTDDHTAPGLVPTDLNLIS
                     AQTFTQISRRDGTDKRGRKSAWFLYQKV"
     gene            complement(17860..18057)
                     /locus_tag="PL000_12025"
     CDS             complement(17860..18057)
                     /locus_tag="PL000_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC89673.1"
                     /translation="MNGEVSIGKRIHFIRLNIQETAGMELAPLYNFEFTPTFIFFDAQ
                     GNEVWRQVGEFDPQLVRDSLK"
     gene            complement(18106..18360)
                     /locus_tag="PL000_12030"
     CDS             complement(18106..18360)
                     /locus_tag="PL000_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89674.1"
                     /translation="MLSLFREYSFLALAIGLVTIAGLILLTNKPRWNDYLAFVVVVAS
                     LVAAWVVLHPRQTELMGDAVTVKELIGAGTPVLLEFQSPF"
     gene            18596..20677
                     /gene="fusA"
                     /locus_tag="PL000_12035"
     CDS             18596..20677
                     /gene="fusA"
                     /locus_tag="PL000_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC89675.1"
                     /translation="MKEYTTEFLRNIALVSHGGAGKTMLAEAFLHATKATTRMGKVED
                     GTTVSDYDDEEHRRKISIYSSIIPIEHRDHKINVIDAPGYTDFVGEIISALSVADGAV
                     ILVDAVAGIEVGTEIAWRYADEFNLPRFFVINKMDRDNANFEATYDAIEEFVKAHGKR
                     AIKVHLPIGEKQNFKGVVDIIGMKSYMGDGNTTADIPADLKEAADAAHFAMVEAAAEG
                     EDELMEKYLENGSLTDAEMVRGLEDVVYAGSFVPVFCAAGGHEVGAIALLNDIIDLMP
                     SPLNAPKRVAQGKGGDEELKPSDSGPLAAYVWKTTADPFVGKMTYFRVFSGSIQSDAH
                     VWNQNKGADERMAGLHLQRGKEGIPVKVIHAGDIASVSKLTATATGDTFCDKNHPLTV
                     PQPKYPAALYRVAVAPKTQADAAKISPTLTRLCEEDMTLTWENDMITHETVLKGMGDQ
                     QIDVALHRAQSKFQVGLLVHEPKIPYREGITRKAAAQYRHKKQSGGAGQFGEVHLRIE
                     PLPTADFEFTDELVGMNLSKSYLAPIEKGIKATMERGAFAGYPMSNVKIIVYDGKEHE
                     VDSKPVAFEIAGREAFKLAVQDAGPVLFEPIMNVRVIVPDAYMGDVMSDLNTRRGRVQ
                     GTETERGTTTVIAHVPMAEMMRYTTQIRSITGGRGYFTMEFDHYDTVPSHVAGPIIEA
                     HKKEMEAKKDE"
     gene            complement(20770..21609)
                     /locus_tag="PL000_12040"
     CDS             complement(20770..21609)
                     /locus_tag="PL000_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89676.1"
                     /translation="MPQYKVVAGTFHVKGFQPDGDSIRFQANKPEHWDFFEWETEADK
                     AAKKKQLRVESIDALETHYEGYHQPRPFAIAALESLLELLNIKSVTYSLSVTQIVDAD
                     DGKAGFIASASTDRFGRPVSYLFPKNAKLTDGAVMDSENLPIEDSINFQLAREGLVYP
                     TFYTTTDRIFAEKIRAVVARARKTKRGIWSIDKTSDFTLLDIRALQEDILLMPKLFRR
                     LVGFFDNYSDFGKLEEYMAKQRDNLVLWDGTKKKSLADLMKITGRRLQMKTPVEDILF
                     SPK"
     gene            21687..21959
                     /locus_tag="PL000_12045"
     CDS             21687..21959
                     /locus_tag="PL000_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC89677.1"
                     /translation="MPTVRFPALMKFYVDNQTEFEVQGATVAEVLENILVRYPALKPH
                     LYDAKGELRRHFNIFVNGVHLRELNGLATELQTGDKIILMASAAGG"
     gene            22272..23234
                     /locus_tag="PL000_12050"
     CDS             22272..23234
                     /locus_tag="PL000_12050"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNC89678.1"
                     /translation="MALLDIRLTQSNTIPLDSLAYHVGNTPLLPLRRVTAKLSPRVKV
                     FAKAEWFNPGGSVKDRPALNIIQNAIINGNLSGGKRLLDSTSGNMGISYATFGAALGI
                     PVTLALPASASPERISILKALGAELVLTDPLEGSDGAILKAREMVAENPDLYWYANQY
                     SNEANWQAHYLSTGPEIIAQTNGLVTHFVTGLGTSGTLMGTGRYLREQLPDVKILAFQ
                     PDAPFHGLEGLKHMPSAIKPGIYDESVAGNPLEVQTEAAHEMVSRLAREEGLFVGISS
                     GAAAVAALQVASELDEGVVVTLFPDAGYKYLSDKSLWEGKAGGR"
     gene            23278..23691
                     /locus_tag="PL000_12055"
     CDS             23278..23691
                     /locus_tag="PL000_12055"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HNC89679.1"
                     /translation="MNLKLTKNILDDISKHVEAAYPEEGAGFLLGVEGEVKQILSLPN
                     AREDEARHNRFLFTPEDYLKAELKADSLGLSLIGVFHSHPDCPNIPSEYDREWAQPFF
                     SYIITRVDEGRSVSHRSWRLVEDRSKYEEEEISIK"
     gene            23709..24203
                     /locus_tag="PL000_12060"
     CDS             23709..24203
                     /locus_tag="PL000_12060"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HNC89680.1"
                     /translation="MTSQTLQKVDHSALKANQYFIISLGVLAFVFNQPILAAFIALMM
                     GLGTILKKPGFGFIYTSLLKPRGWMKPDVLDDNPEPHRFAQFVGFLFMAGGSAALFLG
                     STVLGWTLVWIVVALASLNAFGGFCVGCAMYYWLVRLNVPGFAKQPPAGTFPGMKPKA
                     GATA"
     gene            24200..24607
                     /locus_tag="PL000_12065"
     CDS             24200..24607
                     /locus_tag="PL000_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNC89681.1"
                     /translation="MSSDILLRFAFAIGIIFLGVGVNWLFNKRLISRARNGIGSLFNT
                     PPNKPTIVYFTTPDCAPCKTVQRPALEKLTSLFGEKLHVVEIDATQQPEVAKTWGVMS
                     VPTTFLLDSRGEARYVNNGVARVEKLMEQIQTL"
     gene            24726..>24988
                     /locus_tag="PL000_12070"
     CDS             24726..>24988
                     /locus_tag="PL000_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC89682.1"
                     /translation="MPVLRIPTPLRAYTSGQAEVTVNGSNISEALTDLTNQFPTIKPH
                     LFNDGGELRPFVNLFIGEHNIKDLQGVNTPIKDGDKVMLIPSIA"
BASE COUNT         5392 a         6267 c         6592 g         6637 t
ORIGIN      
        1 gggaattgaa cccgggtccg aaagattcga ccctcggaac tctacgagcg tagcccaccg
       61 atttttgtca tcacagagat ctcagcgggc aggtaaccct gtgatcgatc tgctggtctt
      121 gggcgtattt agcagagtca tacgcagcac cccgcctttg tttcgcccgg tccgtcaccc
      181 ggcagggagc ggtgacggtg gacgcaatct catctaggag attgactgtt atgtctcacc
      241 ttaggcggcg aggggcatag cagcgtatga agtgcgattg gcacttaatg gttgcgctga
      301 gtttacgagt tcggcgcgct ctcggctcgc attccggaac cagcctctcc cgtcgaagcc
      361 gatcatcccc aaatcggcac gggggcatgt gcccgatgca catttattat agcatgggat
      421 tgaggtgtaa tctgctgact tgtccgttat aattggatat gctagactgt attcaggtaa
      481 cagaggcgaa aaatggctga aaaaatattg atcattgatg atgacgtgga taccctcaga
      541 cttgtcgggc tgatgttaca gcgacagggt tatgagatca gtgcggcttc gaatggcaca
      601 caagggcttg cgaaggcatt ggaagaacgc cccaacctga ttttgctgga tgtgatgatg
      661 ccggatatgg atggatacga ggttacgcgg cgtttacgaa agaacccggt aactgtaagt
      721 attccgattc tgatgtttac agccaagacg cagttggatg acaaggtgat cggttttgaa
      781 gtgggtgcgg atgattacct gaccaagccg acacatccca ccgaattaca ggcgcatgta
      841 aaagttctgc tcgcacgcca gaaggatgtg actgagatcg tcacttcttc aaaggagcat
      901 gtaggatatg tgattggtgt gctttctgcc cgtggcgggc tgggggtttc ttctgtggca
      961 gccaaccttg cggctggaat tcatacccgt attaaagcgg atgtgattct tgcggaactt
     1021 acgccggggc agggcacttt gggtatggat ttggggtcgc cgaatcaaaa gggactcacc
     1081 gaactgttgg aggggaccac tgcggaagtg acacgcgaga aggtgaaatc actgctcgtt
     1141 tcgcataatt ccgggttgaa gttgatgctt gcctctgaaa atccacggga tgtaaccttg
     1201 aattcgaagg tgccgaatta tgaggcgatt gtggctcaac tttccacgtt ggcgcgtttc
     1261 gtcattctgg atttggggaa tggtctgccc gtccatgtgc agaaactgct gcccatgtgt
     1321 actgagcgta tggtcgttgt ggaaggggtg cctggttcca ttcaacatac ccgcctgctg
     1381 attgatgaga tcgccaattt gaagatagac agaaagacca ttacggttgt tcttaataat
     1441 cgaatgcggt ccgaagcaca gatgcagttg aatcaggtgc aggaattgct ggggcattcc
     1501 gtttcggcca cccttacccc tgcccctgaa gcatttttgc aggcaacccg cctgcaaacc
     1561 ccggttgtga tcgggcagcc ttcgaatatg acctcacagc aatttttgaa attatcagat
     1621 gcggtccttg agcgtgaaaa ggcgcgatga catccttttc ccctcagaca caagctgcga
     1681 ttgagttcgc tgcggatttg ctccgccaat ccagccatgc tgttgttctg acgggcgcag
     1741 gactttccac cccttcggga atacctgatt tccgttccac tgggacgggt ctttgggcaa
     1801 aggatgagcc gttggaggtg gcttcgctca gcacctttcg tacggcaccg gaacggtttt
     1861 ttcaatggtt tcgaccgctg gccagccaga ttttcaacgc ccagccaaac cttgcgcatt
     1921 gggcgctggc cgagcttgag aaggcgggac acatagattc catcattacc caaaatattg
     1981 ataccttgca tcaaaaagca ggttcaaaag ttgtgatcga aatgcacggt accatgcaaa
     2041 ctttaacgtg tacccagtgt tatcatcaag tggatgcagg gaattatctg gctgcatttg
     2101 tggagacggg agaaattccg ttgtgcccaa aatgtggtca ggtgctcaag ccagatgtga
     2161 ttctgtttgg tgaacaactg cctcaaacgg cgtggttcaa ggcccagcgg gccgcccgcc
     2221 aatgtgacct gatgctggtg atcggttcat cccttgaagt tttaccagtg gctggtttgc
     2281 caatgcaagc cctggatcgc ggcgctcatc ttgtgattgt caacaataca cagacctatt
     2341 tgaacgtgcg cgccgatgcg gtgatcccgg aggatgctgc agtgaccatt cctgcgattg
     2401 tggagagagt gctacatggc tgaaataaaa accgaacgat tggatagtta tcgccgtctt
     2461 attgatattg cgcgggatct cgcttccacc ctggacctcg atatcctgct gtctcgaatc
     2521 gtacatgctg cggctgagat cagcggtgct gaggccgcct ccattttgct gtatgacgat
     2581 acttccagac agttatattt ccaggtctca acaaatcttg atgaatcaac ccgccgcggc
     2641 attgtcgtgc cgttggaggg gagtattgca gggtggatcg tgaacaatcg ccgccccgtt
     2701 cgtatcacca atgcacatga agacccccgt ttttactccg atgtggaggc gaccacgggt
     2761 ttttccactg attcgctttt gggcattcct ctggtaacca agaacaagat cgtgggtgtg
     2821 ttggaagcgt tgaacaaaca ccgcggcaaa ttcaccgact ccgatgaatc catgctgctg
     2881 gttttgggtg ctcaagcagc ggttgccatt gaaaatgcac gcttgttcca gcaatctgac
     2941 ctgatctctg aatttgtaca tgaattgcgc acgccgctct cttctttgag tacggcaact
     3001 tatctgttgc tgcgcccgga gatgtcgcag gagcaacgcg atcagatcat taataatatc
     3061 catagcgaaa ccatgcgttt gaatgcattg gcttcctcct tccttgacct ggccaggctt
     3121 gaatctggac gggtacagtt ccgcaagacc accttcagcg tggcagatct catttacgaa
     3181 tgcaaggatg tgatgtcttc caaggcgatc gaggataata ttcagatccg cgttgaatcg
     3241 cctgagggcc tgccgctgct tgaagcagac cgcgaaaaga taaaacaggt gcttttgaac
     3301 ctgttgagca atgccatcaa atacaaccgc ccgaacggga cggtgatgct ccgcgccgaa
     3361 ttgaccgaaa aagaactggt catttacgtg caggataccg gccttggtat cccggatgag
     3421 tccttgccgc acttgttcga aaaattcttc cgtgtgcgtg aacacgagtc gcgcattgcc
     3481 ggcaccgggt tggggctttc catctgcaag cagatcatcc acggtcatgg tggccgcatt
     3541 gaggtgaaga gcaagatcgg tgtggggacc gttttttcca tcttcatgcc ccgcactgga
     3601 aaaggccagc cgctccccga agaccccctg gaccccaaga aaatcatcaa aaaatgataa
     3661 attggcctcc tttatggtgc cggcagcgaa gtatcgtata atttaccaaa acagaatcat
     3721 gatattcgtc cgcaattttc gaaaaatttg ataaattgtc ggagcaagac agtaattttt
     3781 tctttggagg aagatgatgg ctcagtttca atttgttatg aggtcgggac cgacgcccgg
     3841 cgtaactttt cccctggagg gcgatcaact catcattgga cgtgacgcat ccaacagcat
     3901 tacgatcaat gatgcagaga tttcacgcaa acattcccgc ctctcttttc aaggcggcaa
     3961 gtatgttctc gaagaccttg gttctacgaa cggtaccttt gtaaacggac agcgtttggc
     4021 gggaccggtc gtcctcaaac ccggtgacgt ggtttccctc ggtgaacaga tcgtcctgat
     4081 gtatgacgcg atcaacattg acaccggtgc cacgattgcc acctcccgaa aatctgcacg
     4141 cgtagaaccg ccgccagcgg ctgctgtatc tcaaccgcct gctccaaccc ccactccggt
     4201 ctacactcct gcaccttctt attccggtcc gccagccgtc aaaaagacga atatgttgcc
     4261 catattcatc ggtggcggcg tcctcatctt tgtctgtttt tgtatcagcc tgctttggtg
     4321 gatagatgct acttatcggt ggtgtgtatt cttccccttc ctcacaggtt gttaatccaa
     4381 aaagattttc aaagcccgcg caggtacagc ctcgcgggct ttttcttttc ccatccgcct
     4441 gccttatggc tttctcaaat taatgtctga caagcctttc gaaccgccaa gcccgcgaag
     4501 aacgccaaga aaatccatta aacttcgcgc tctttgcgct cttagcggtt agttttgtct
     4561 atgttgggca agtactcccg actttgagaa agccataccg cctgccttgt gaaatgttgt
     4621 aaaatcaccc atctttgata gaatcgaact gaacccgtaa acgacgcgta ctggaaccaa
     4681 taaccttatg accgaacaac aaatcccatc ttccccggta tggggcacaa ataccaaact
     4741 tgtcgtggcc ctcacagtgg ttgtgatcgt tggcgcgttg ctggtcaagt ttcagttcat
     4801 tttgaccccg ctgttgatcg ccctggtgct ggcctattta ttccacccac tttccagttt
     4861 tcttcaacgt aaactccacc tttcctgggg ggcttcggtc ggtattgtat atctggtggt
     4921 catcatcctg ctgttggggt tgcttaccct tggcggggtg agccttgtac agcaggcaca
     4981 aagcctggtt tctgtggtgc aaactgccat acgatccctc ccagctttca ttgacaggat
     5041 ctctgacaaa atctatacct tcggcccctt caaaattgac ttccgcgcct ttgacctcac
     5101 caacctcggc gaccagatcc tgggcatgat ccaacctttg ctgagccgaa cgggcacact
     5161 catcagcaca gtggcgggtg gcgccgccaa cttccttggc tggactctgt tcgtcgtcct
     5221 cgtttcttac tttgtgttgg ctgaaagcgg cgggtttcgc aaccgcatga tcagtgtgga
     5281 cctgcccggc tacacccacg atttcgaacg cctctcccgc gaactgggcc gcacctggaa
     5341 tgccttcctg cgcggacaaa tcatcatctt ttttattgcc atcatcgtgt acaccattgt
     5401 attgagcgta ctgggcgtgc attatgccct cagcctggca ctcatggcag ggctggcccg
     5461 ttttgttccg tatgtcggcc ctgcggtcaa ctggggattg atggcgctcg tttcatactt
     5521 tcaggtttac aaactgttcg gcctttcacc agtgtattat gcgctgttgg tgctgctcat
     5581 cgctgtcatc attgaccaga tctttgataa cgttgtcacc ccgcgcatcc tttccgactc
     5641 gctaaaagtc caccctgcgg ccgtgttggt tgccaccatt gttgcggcca acctgtttgg
     5701 tttgttgggt gtggtcgtag ccgcgcccat cctcgccacc gccgccattt tgtggaagta
     5761 catcatgcga aaaatgctgg atttggatcc ctggcccgaa gaggaacaga ctcaaccaac
     5821 accgcctccc gggtcaagaa tcctcgtctc gatccgccgc ttctttcgga ccctcagcct
     5881 gaatcgcagc aaagggaaat aaccaccccg tagcattgac aagcggggcc gcgcgcccct
     5941 gccaaccgct gaaactcatt catcattcaa tagacccaag gagaaacatc atgagcaata
     6001 acgaacccaa aacaacgacc atcgccgaaa ccgaaaattt catcgcctgg cgcgctgaag
     6061 aacccgatgg tgaaaccacc taccacgttg aattaaataa tgtgaccatc cacttctttc
     6121 aggaagaatg ggtcgaattc ctcgaactcg tccgctcgtt gaaatagaat ctttgccgta
     6181 acgacttcag cctttgtttt atggggctga ggtcgttacc cttttttgga gtccactgaa
     6241 tgaaattatt ccagtttctg cttcgggtgg gggggagttt ggcagttgca ctaatcgtca
     6301 gtttttcctc ggcggtgatc ttttttaatt actctggcta cgaaaacctc tcagaccgca
     6361 tttttttggt ggttgtaccc acccttgcca ttgggtatct tttctacgaa tcatttccaa
     6421 aatactggcc ctggcttcta caaagacaac ctgctgtgct ggtgaccttt ggcctgctgg
     6481 cattgctggc tgctgctgtt gttgttttgc ctgcggcggt ttcgaacgtg tattatcttg
     6541 ggctggtttc actggctttg gccttgttca ttctcatgct gcctgccgca cccgctgtgg
     6601 agcggattcg cacctcgcat tcgatgctgc attacatgct cggtttcgtg ttgaaccttg
     6661 ccgtctctta cggggtggtc ggtttcatgg gtggcgtgtt cagttatcgg tttgatgtga
     6721 tcgttttttc catcctcatc acgcttgtag gaagtgtcat cggctattat ctcgtccgcc
     6781 gtgcatcccg ttccatgcgt gatggatttt tgcgtaaccc gcttaatttt ttcctggtta
     6841 tttcgctgcc tgcttttttt gcggccatgc ttttcctgtc gctgcaattt ccatccatgt
     6901 tctcgctggc atacattcaa attccgctgg cgcggtttgg catgttcata tccagcgcgg
     6961 tggtggcagg cgcatggagc gttgccctgc tggagcaggt ggaactgcgc ggttggtatg
     7021 cggccttcaa gcagtcaaag gcttacgcct ttatcaatga aaacctgcct ggcctgtatg
     7081 ccgccgcatc gttttttgga atcaacctca tcattgcccg cgcattgaat caccccacct
     7141 tcagcatcaa cagcattgtt ttcgaagccg atgcgggtcc gtggatgtcc attctcggtt
     7201 ttcccgaggg acacgatgtg aatcgcgccg tacatccttt ggtgctgatc accctgcggc
     7261 ctctggttgg cctgatccaa atattcactg ccgataaatg gttcctcggc cccatcattg
     7321 ccgttgctgc aatgaacgga ctttgcatct tcatggcctg gctttttgca aaacgtgcca
     7381 cgcaaaaaga ttcctacgcc tttgtttttt cgatcattct tggtgttacc gccgcgcatt
     7441 tgctttttgg ctcgctgact gaaacctatg tctttggcat gacctcattg atcctgttct
     7501 tcgtcctcgt gcaggccgat gaaaaaaggt tttccgtgct ggtgcctgcg gggattctcg
     7561 tttttggggt gacggtcacc aacattgcgc aaggcatgat cgcactcttc ttcaaaaaat
     7621 tcggtttttg gaaactcgtt tattacggca tcattgttct gaccatcagc atcgcattga
     7681 ccgcttttgt cagtgtgctg tacccgggca accaaacctt cttctgggtc cctgcagatc
     7741 tggcctttga aggacgtttt tccaaaccca tttacgcggg gccgatggat cgtgtcgttg
     7801 aaaagttcgg cgctgttggc cgtgccatct ttttccacgg ggttgttgct cccacaccca
     7861 ttgaagattt cacccgcaag accaccgatc cgctgattga tttcaagacc cacgattatc
     7921 acgaacaccg ctatgcctgg tacaacggcg cggcctattt gccggtggcg ctctggcttg
     7981 gtttgttcgc aggttcggtt ctactcttct tcaaaaatct gcgcacctcg ccgcatattc
     8041 cgctgatgtt ggggctgctg ggaagtatcg gttttaccta tttcctgcac atgaattacg
     8101 gaaccgaact cttcctttac tcgccatatt ggacctacct gttgattttc ttcctcatgc
     8161 tggcctatgt ggattttgca gggaaacgct ggttcgaagc cgtgctgacc gtttttgcgg
     8221 tgactttgat ggtcaacaac gcctggttca tcttcatcat cctgcgtgga cttgccccct
     8281 acctcgctgc aggctaatca cagcggacct cgcgcctgat tccgttcaaa ataatgaatc
     8341 agcccctcag gcttttcctc actactttaa ctgcattggc ggttgccttt gcgttcagct
     8401 ttttcttcct caatttgtcg ggtcgttctc tggcagagca aggattgctg attggatttt
     8461 tggccgcatc gttggggtgg ttgatttgga atgcaccctc tgttctcagt gaatggaagt
     8521 ttgccatgcc caagctgggg ctgcgtgaaa acctgcccgg tctcctcctt gcattgacct
     8581 tttttgcagt gtacacatcc attggcctgc agctcaacac cctcagcgta gacaccaccg
     8641 acaacttcct cgacgccgat aactcttcct ggatcacacg cattgccgac ccggcaggtt
     8701 ccaccctcga aatgcgcgga ccacatccgt ttgcgtattt tattttccgc ccgtttggct
     8761 ggctgttgaa tctcttcacg gggagttctg ccctctcagc caccttgttg aacacattca
     8821 caggcgcgct gtgtgttttc ctggtctggc tgttcgtgaa gcgccaaatt caagatgcgg
     8881 tgtatgcctt gttgattgca tctttattgg ggctgagtac agcgcacatt ttcttcggct
     8941 cggtcgtgga gacttatatt ttctccgccg cggcgctgat cgggtttctc cttgtccttc
     9001 aaaaaggatc cagttccctg cctgcttctg ttggagtgag cgtgttgacc ttcggcatta
     9061 cactgacgaa cttcgttcag aacttcattc tatttgccgc tgcccagata caggcaggca
     9121 cgaaaaattt caagaccctg gtgctgaacc tctttcgttt tacagccttc actcttgcgc
     9181 tggggatttt gctcagcatt ctccatgccg catggtttcc ttcgagcagg ttgtttttcc
     9241 tgccttccga tgcgcaggcg gaggaggagt ttgccttttc tgtgtttcag gagccatcgt
     9301 ggaaggtgat cggacgtgtg tttttactgg tgcgtacggt gtttttgtat gcggtcattg
     9361 ctccgcagcc gtatgtgttc ggcgaggagg tgggggggaa gtttccgcgt tttaactttt
     9421 tccgcatttc tccagagaca ttttcctata gttcatacga tggggtgggg aatgtgcttg
     9481 tgctggcttg ggctgtcctg cttttggttt caggcttctt ctttttgagg aatctggtcg
     9541 gcacccgcaa agttgacctc tctgctgcgt ttatcttgtg cgttctgttc aatttcgtcc
     9601 tgcatttcaa ttatgggtac gaaatctttc tgtactcccc cgattgggcg tatgcattga
     9661 tccttttcgt ggcgctttcc cttgcgccgt tggcaaaaaa ccgtttcttt caaggtggtc
     9721 tggcggtctt tctcattttg cttgcttata atcagtttca gttctttgat tttattttca
     9781 aaaccattgc gttattcttt gacaaaggtg cctgatgttc caattgctta atcatcgcat
     9841 gctttttgcg tggatgttgt ctgtcattac ctcgtatttt gtggtcgggt ttctcggtaa
     9901 ttattacccg tcctttgtag gggtgcttgt gctggcggtc attgcccaaa ccctgttggg
     9961 agtgctgatc gaccgtttgt tcgccaaggc ggagcggttg tattcgtcca ataaaggtga
    10021 cctgtttttg gcgctggcgc ttttcatggt gttgactgtt ttcgcggcgg ccatgcttca
    10081 gatggcgaac caatttccga gtttgttcga tgcgaagttc gtcctccttg aacaggacca
    10141 gacttttccg ttcatcatcg ccggggtgtt gaccattccg cttttggctt tggcagggca
    10201 attcatgggg cagtcgcggt tttctgcttt tgtcgattcg atcgcaacgg gtgtgctggt
    10261 tgcctccttc ttttttgcgg tctattttat tttctcgttc attttcaacc agcctgtttt
    10321 tgatacagac gatattttct ttgattcaga cggctggttg tggcgcacac gcttcacgac
    10381 agatgcttat gaagattatt actggcgttc actgcatccg ttcgtgttgt tggtgattcg
    10441 tccgctggtg tttttggttt cactttttct aaaaggtgat cggctttcgg cggggttgtt
    10501 gttgatgcct ttggcggggg cggcctgtgt attcatggcc tggtattttg taaagaaggt
    10561 gacgggcaat tctttatacg cggttttgat cgcggccctg ctcggcggca caactggaca
    10621 tttgatcttc ggctttttga tcgagaccta tatttttatt gcggctgtgg cgatgctttc
    10681 gatcattttg ctgttgaggg atgcgcctct gttcacgttg atcatcacgg gactggtttc
    10741 atttggcatt acgctctcga atatcatcca gccgacaatt gctcttgttt tcgtgcggcg
    10801 tgatcttaaa cagtggatca aatacggcct gatcgttggc gcactggctt tcccgctgac
    10861 cttgctcaac aatgtgatct accctgattc gcagccgtat ttcttcgatc catcttccta
    10921 tggcacggaa ggacgcaata cttttgaggt gcatccagcc cgggcggtgg cgatcggacg
    10981 ggtgatgttc ctgaacagca ttgtggcccc cgacccgctg atcttgaaac gggaattgcc
    11041 gtttttgaaa gtgtggatcg ttgaggtcag ccgcaagttg tatccgcagg aagccaaccc
    11101 gatgcgcagc agtgaatacg aaacacagtt tggcacggca atggtggtct tttggatggc
    11161 gctggcggcg ttgggcgggt ttggtttttt gaagaacatc aaaaagcagg ataaccgttt
    11221 tgcctatgct tttatcttca tcaccctgtt cagctttgtg cttcaccaaa aatttggcaa
    11281 ggaattgttc ctctacgcca ccaattggac gtatgcgatc gtcctttttc ttgcgttggg
    11341 gtggcgggaa ttcatgggca ggcgctggtt tcaggcggtg atgttggttt tcattgcctt
    11401 gatgcttgtg aataactcgc gtttgatcct gacaatgttg agcacatcag ctctgcatat
    11461 tagatgagtg cgacgcactt cggactggaa tcaaaaagag gtttggcatt tgcgccaaac
    11521 ctcttttttt ttatatttct tgtgactgtc ttacgacttc ccctttaatt catcgcggaa
    11581 gatgacactc atcacgatgc tgacgatgct gatgacgagg cttccgagta ccgcggtcca
    11641 gaagccatcc acggtgaggc cgatgccgaa gctttgcgca attttgctgg tgagcaggag
    11701 catgaccgtg ttgatgacga tggtaaacag gccgagggtg aggatgatga gcggacaggt
    11761 gaggaacttc aacaaaggcc gcaccagtgc gtttaataag ccgatgataa gcgccagcca
    11821 gagaataccc gtccatgagc cgcgcaggtc aatgcctggc acgaccagta ctgcaacata
    11881 caaagcgacc gcgttgatcg cccagcgaaa aaagaattta gtcatttgaa ctcctttgtt
    11941 tgtaaatacg caaaatgcgt tacaaggttg cctgcatcca gatcaatgtg cgttgggctt
    12001 tgaaccctgc ggcttgaatg gcggtgtcaa attggctgga tgggaattcg agggtgatgc
    12061 tgggatggga atggtaaagc tcgcaccttg cttcgagcag gagagcggtc aacgcatcgg
    12121 ggttggaccg ttcgccgatc gctgcaaaaa gggcatcgcc gtggccctgg ggaatccagg
    12181 cgagtgtggc ttcgaggggg gcgggttgct gcgggtcggt ttcttttttt gcagcggccc
    12241 attgacgtat gttcacgtca atgaaggcca ggtacaacca gttccagaac ccgggcttga
    12301 ggggagagaa tgaccaattg cggtgccagg ccaacggttc ggggtagagg cgggtaagcc
    12361 attcgtgttg gatgggccag tcgcgtggat tccgctttgt gatgatgaag ttcgtttgca
    12421 gggaggtcgc gtgagggtcg gtgctggcga tccaggatgt gcgccgtgcg cgttctacaa
    12481 atccgagttt ggtgtagagt tcgatcgcgc cggggttgtc atcgcggacg tgcagccaga
    12541 tgttgttgat ctctttttcg tgggcatgct gcagggcgcg ctgggtgagt gccctggcga
    12601 ttccgcggcg gcgataatcg ggatgaacgg cgatgttggc gatgaggtag atgcgttgtt
    12661 tgttgtgttt gaatgggacc aggctggcgt tgccgacgac ttttccgttt tcctcccata
    12721 tatacccgct cagcggcagg gatgtggttt cgatggcacg gttggcccat ttgacaaagg
    12781 agttgtcgtt tccggcgcgg cgcatgtctt gcacgtagcg gcggccttca ctatccatcg
    12841 tgctggagaa acaggtctcg atcaggtcag ccacggcggg aaggtcgcgt aacacattga
    12901 gcggacgcag gttggtgtgt gtttcggtcc gcgctggaat ggtgatggaa gacattgaag
    12961 gcattataac gctttgtgac cgagggtatt atataatcga aaaaaatttg aactggagcc
    13021 ttcattgaaa aaacattccc ttttcactgt gttgctgagc ctgcgcggaa acccgcgcgg
    13081 ttgcgtttat tttgagcctt tgtgggggat tccgtataat ttatttgctc cgtatgtttc
    13141 gatctatatg atcgcgttgg gaatgtctga aaagcagatc gggttgatcg tgagcatcag
    13201 ttggggattt cagatttttc tggcgttgat cagcggggtg gtgaccgata aattggggcg
    13261 gcggtttaca accctggtct cggatatttt gtcgtggacg atcccttcga tcatctgggc
    13321 ggtttcgcag aatttctggt attttttgat cgcgggtgtg gtcaacagca tctggcggat
    13381 ctcacatact tcgtggagtt gccttttggt ggaagatgcc gagcaggatc agttggtgga
    13441 tatttacacc tggatctata ttgccaacct gttcgtcggt ttcattgcgc ctttggcggg
    13501 ggtgttgatc ggggtatttt cgttcgtgcc gacgatgcgt ggattgtatg tttttgcagc
    13561 ggtgaccttt acactgaagg ctgtgttgac ctatctgtgg acggaggaaa cagcgcacgg
    13621 aaaggtacgc atgcaggaaa cgaaggatca gagtgttttg catgtctttg gcgaatacaa
    13681 gggtgttttg cgtgacctgg tgcgtgcgcc gcaaaccctg tacacagcgg ggatcatgct
    13741 ggtgatgagc attacctcgt tgatcaacgg cagtttttgg tctattttgg tgactgagaa
    13801 attgcagatc gccactgaga atttggcaat attccccttt gtaaaatctg ccatcatgct
    13861 ggtgttttat tttacggtga tgcctgccat cggcaggatg catttcaaaa tgccgatggt
    13921 ggtggggttt ctggggtatg tggccagcca ggtggtgctg gtgaacgcgc cgagcggcgg
    13981 atatgggttt ttgatcttca gcgtttttct tgaggcatgc agtgtggcgg cggtcagccc
    14041 gttgatcgat cagatgatcg ttctgacggt ggatgcgaag gagcgcgcgc ggatcctgtc
    14101 cattttgtct gtgggggtca ttttggtgac atctccgttt ggctggatcg ccggtacact
    14161 ctccacgctt gataaggatc tgcctttcct tttgaacatc tctctttttg tgatcggcat
    14221 ggtgctggcg tatttcgccg ggcagttctc acagaagaaa accgtgatgg cggaagcggc
    14281 aggttaggaa aatgtatgtg aaaattgtag ttgtgtattg aaatcgggtc gcctgtccat
    14341 atggacaggg tgcatcaggc tgagcaggct gccgctttca cgccttccgc cctgcctccc
    14401 actcccgcac gcggacaaaa agttccccca gcggattcaa gctcgaaaca ggtgtggttc
    14461 tcgattactt tttgtattcg ccccattcct gttgaggtcc gtcaagcagc aaacggatat
    14521 gattcaaaat attcctccct aaaatcccat cggggcgatc aatcaaaaga tattctccct
    14581 tgaaagtctt gttgaaaaca agcagatcga gtcttgcaac tcttaataac tttgtttccc
    14641 catcaaatac ctgaacttca aattcagaat ccacttcgat ttccacacca agttgctgac
    14701 tgcactcaga ggtaataagg tggcatctgc acccgtatca accaacaagg ggacattggg
    14761 tattacagtt ccattttgag gatttcgaag ggttacatag gcaataggcg cgggtggatc
    14821 ataatttttc gaatcgtatt caagcattgg gcttttcctc agtgacggtc attttgaaac
    14881 gagccgcatc gtcacgtttt gccaaacgcg gtgagacgat ttgaacaggc ttcttcccgt
    14941 tgatctttgt ggaaatgacc tcttccgtaa cgatcacata gacctttaca ttttcgggca
    15001 gttgaacgcc ttcgcttaaa agaatactgc ctttttggac aacaccttca taagttacaa
    15061 gagtcatggg gcacctctga agaaaattat agcatgggtg ggctgctgcc gctttcacgc
    15121 cttccgccct gcctcccact cccgcacgcg gacaaaaagt tcccccagcg gattcaagct
    15181 cgaaacaggt acagcgagat tttttgtttt ggtgcggtca tattgaaaca cgcgtgaatt
    15241 cggtgcgatc aactttgcgc ggcggcggaa ttcttcacgg gtgatccagc ctgtcagtgt
    15301 gatatccatg ccgtagagcc acaggttgcc ccactccaac ggtgcaatga catgcgcctc
    15361 tttgaaagct gggctgtgaa tcccgatccg cgctgaaggg atgtttctga catggatcgc
    15421 catgaccgaa taaaacgaat catccacaat cacacgcact ttggggggca gactgagaac
    15481 ccgcgtcaca aatcctcgac cttcatcctg tccgctgatc tcgacgatca attcctcatc
    15541 tgattcagat ttcaagacca acggcgccag cggagtccat tgatagggcc gccgccatgc
    15601 ttcgggcatg aggtggatca aatagcgcgg ctgattggtt tcgatcacct ttttcaggtc
    15661 atcttgcgaa gcggcgatca ggcctgagag gaaggcaaac agatacaggt cattatccgc
    15721 gcgcccatcg ttgacgtgat gctcggaagg caccagcgcg ggggcattca gaatcaacga
    15781 cgggtcgctc cgcatttgcg cgatctggtt gcgatgtgaa atgaaaaagg attgaaggtc
    15841 gcagcgccgc ccgcccagcg aaacgtcata ttgatcgggg ttggtgaatg gatttacgct
    15901 ggcgatctcg aatggaattc cctgctgcga aagatggcgc cgaaacgcca attccactgc
    15961 tgcgcctgcc accgtgcggc ggagttgatc gtactgcctg ccttcggcgt gatcgaaacg
    16021 gttgggcaga gtccgcagtg catacgcaat tccaccctcc gtaagatcgg gcgtgtatgg
    16081 gaggtggatg aggtcagcgg gggagagcat gggggaaagg aagaacgatg aaggatgaat
    16141 gatgaatgat gaaaaagtaa aaccctaatt cctaatgccc aatccctttt caattgctgg
    16201 ggcaattcct gtaaggtcgc ggatgtcctt tccgaattcg tctcggatgg atttttcccc
    16261 gaataataaa agcggaacat cggcttcggt gtgcttgcgg gtcgagaggt cttccatgtt
    16321 gccatggtca gatgtgagca ggacgaggtc gttgctgtcc caatgttcga gcagaccttt
    16381 cagcacactg tcgaatcttt ccatctggcg tacagccgag cccatatcct gcttgtggcc
    16441 tgcataatcg cttgcccaat actcgaaaaa agagaaatca tactggcggg caaggtcggc
    16501 gagtcttttc cctgcttctt cggtttcaaa gatcggcgct tcggggaagt tcaaaaattc
    16561 atgccagccc tttcctgtga aatctgccga aagcgctttg cctgcaaaat aatcttcgtg
    16621 cgtgaacagc ggcagacctg cattggtcaa cgcgagcgga atggcggaat acaaccgctt
    16681 gcccgaatca attccctcaa aataacgcgg cggatatgca ttcaataacg cggcggtttt
    16741 gcctgcctgc acggtttttg caaaaagcgt tttgccatcg aggtattgcg ccacttcggg
    16801 gttgggtttg ggtccgtagt gatacccgat ctcagcaggg atgttgatgc ctgtcagcag
    16861 aacggcctgc cccgttgccg attgtggagc gccgttcacg cccagcctgg ggttgatgga
    16921 aaacagcgca gccgactcac tgtcaactgg tgcagattca cggagcagtt tccgcccctt
    16981 gagcagtgac tcaagatacg gcatgtttgc gcgtgcaaag ggattgatct cagggttgtt
    17041 ttcgccgagc ccgatgccat caaggaagag gaagaggatt ttcataataa aggttgaagg
    17101 ttgaaggttg aaggttgaga atcagatgtg ttatgtgttt ggataattac caattaccaa
    17161 ttacttcccc cttactttct gatacaaaaa ccatgccgan nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnt actttctgat acaaaaacca tgccgatttt cgtccgcgtt
    17341 tgtcggtgcc atcgcggcgg gagatctggg taaatgtttg ggctgagatc aggttcaggt
    17401 cggtggggac aagcccgggc gcggtgtgat cgtcggtctg gcggaagaat ccgtacatga
    17461 gccagaatcc gttgggggcg aggattcggt ccagttgggt cagataatcc gctttgttgg
    17521 ggatgccgtg gaaacaaccg atgtcgagcg caaggtcaaa ttgcccgctg atgtttttca
    17581 tttgggtcgc gtcgtcggta aagaggtcgg cttgtatgcc tgcggttttt attttgcgct
    17641 tcgcaatgcg gatggcacgc gaggcaaagt cgaagccttg cacctgccag cctgttttgg
    17701 cgagtgtgat gacgttggtg cccgtgccgc agccgatgtc aagcgcacgc cctgctgggt
    17761 ggctttggat gaattcgaag agttcgggcg gggtgatgcc gctatcccag ggcgggcggc
    17821 gtaaatacca aaagttgaaa gagatgcggc gaaagaaatt catttgagcg agtcgcgcac
    17881 cagttgcggg tcaaattcac cgacctgccg ccagacttcg ttgccctgtg catcgaagaa
    17941 gatgaaggtg ggggtgaatt caaaattgta gagcggggca agttccatgc ctgcggtttc
    18001 ctgaatgttc aagcggatga agtgaatgcg tttgccgatg ctgacttcac cgttcaattc
    18061 ctgttcgagc tcgtcaacga cgggctttaa ttgggtgcag gtaattcaga agggagattg
    18121 gaactcaagc aaaacgggtg tgcctgcgcc gatgagttcc ttcaccgtca cggcatcgcc
    18181 catcaactcg gtctggcgcg gatgcagaac gacccaggcc gcaacaaggc tggcaaccac
    18241 cactacgaac gccagatagt cgttccatct tggtttgttg gtcagcagga tcaaccccgc
    18301 aatggtcacc agcccgatgg ccagcgccag aaaggagtat tcacgaaaca atgagagcaa
    18361 gttttcctcc gttttaggtg gagtggaagg tccatattca agaaatcacc ccgaagggtg
    18421 aattttgcgg attaaatgtc atgcgcaggc cgtgacggtt cacactttga caattcaatt
    18481 gagttctttt acaatcgtgc aacttgtcca aaggtttacc gggattcctc tggcttcgtt
    18541 ctggacaggt gaaaagaact gctttacatc ctacaaatat aggaaggcta ctttcatgaa
    18601 agagtatacc accgagtttc ttcgcaacat cgcgctggtt tcacatggag gcgcggggaa
    18661 aacgatgttg gcggaggcat ttttacacgc aacgaaggca acaacacgta tggggaaggt
    18721 cgaagacgga accacggttt cggattacga cgacgaggaa caccgccgaa agatctcgat
    18781 ttattcaagt atcattccca ttgagcatcg cgatcataag atcaatgtaa tcgacgcgcc
    18841 aggctacacc gattttgtgg gtgagatcat ctccgccctg agtgtggctg atggcgcggt
    18901 tattttagtg gatgccgtcg cgggcattga agttggtacg gaaatcgcat ggcgctacgc
    18961 agatgaattc aatctgccgc gcttttttgt tattaataag atggatcgtg acaatgcgaa
    19021 cttcgaagcc acctatgatg ccattgaaga atttgtaaaa gcacacggca aacgcgccat
    19081 caaggtccat ttgccgatcg gtgaaaagca gaatttcaag ggcgtggtgg atatcatcgg
    19141 catgaaatcc tacatgggtg atggaaacac caccgcagat atcccagccg atttgaaaga
    19201 agcagcagat gccgcccact ttgccatggt ggaagccgcc gccgaaggcg aagacgaact
    19261 gatggagaaa tacctcgaaa acggttcttt gaccgacgcg gaaatggtac gcggcctcga
    19321 agatgtggtc tacgctggtt cattcgtccc cgtattctgt gcggcaggcg gacatgaagt
    19381 cggtgccatt gccctgctca acgacatcat tgacctgatg ccctccccat tgaacgctcc
    19441 gaagcgtgtg gctcagggca agggcggtga tgaggaattg aaaccgtctg attctggccc
    19501 attggccgcc tatgtttgga aaaccacagc cgacccattt gtcggcaaga tgacctactt
    19561 ccgcgtgttc tcgggttcga tccagtcaga tgcgcatgtt tggaatcaaa ataaaggcgc
    19621 cgatgagcgc atggctggtc tgcatctcca gcgcggcaag gaaggcattc ctgtgaaggt
    19681 catccatgcg ggtgatatcg catctgtatc gaagttgacc gccactgcca caggcgatac
    19741 cttctgcgat aagaatcacc ctctcaccgt tccccagccg aaatatcccg ctgccctgta
    19801 tcgcgtggcg gttgcaccca agacccaggc cgatgcggcc aagatctctc ccaccctcac
    19861 ccgtctctgc gaagaggata tgaccctcac gtgggaaaat gacatgatca cccatgaaac
    19921 cgtattaaaa ggcatgggcg accagcaaat tgacgtggct ttgcatcgcg cacagtccaa
    19981 gttccaggtg ggtctgcttg tccatgagcc aaagattccc taccgtgagg gcattacccg
    20041 caaggcagcg gcccagtatc gccacaagaa gcaatcaggc ggcgcggggc aattcggcga
    20101 agtgcatctt cggattgaac ccctgccaac cgctgacttt gaattcaccg atgaactcgt
    20161 cggcatgaac ctctccaagt cctatttggc gcccattgaa aagggcatca aagccactat
    20221 ggaacgtggc gcttttgcag gctacccaat gagcaacgtc aagatcattg tgtacgatgg
    20281 caaggaacat gaagtcgatt caaaaccagt cgcctttgaa attgctggcc gtgaagcctt
    20341 caaacttgcc gtacaggatg ccggcccggt cctgtttgag cccattatga atgtgcgtgt
    20401 gatcgttcct gatgcctaca tgggcgatgt tatgagcgat ctaaacaccc gtcgcggccg
    20461 tgtacagggt accgaaaccg agcgcggcac caccacggtc attgcccacg tgccgatggc
    20521 tgaaatgatg cgctacacca cacagatccg ctccatcacc ggcggacgcg gctacttcac
    20581 catggaattc gaccactatg acaccgtgcc ctcccacgtg gcagggccga tcatcgaagc
    20641 tcacaagaag gaaatggaag ccaagaagga cgagtagttc agtaattgag tacgctcagt
    20701 caataataaa cagtcccgaa gccaggtgcc tcgggactgt tttatcttct aacgactact
    20761 gccttcccct tacttcggcg aaaacagaat atcttccact ggggttttca tttgcaggcg
    20821 tctgcctgtg atcttcatca aatctgccag tgactttttc tttgtgccat cccacaaaac
    20881 caggttatct ctttgtttgg ccatgtattc ttccaacttc ccaaaatcgg aataattatc
    20941 gaagaagccc accaaacggc ggaagagttt tggcatcagc aaaatatcct cctgcaaagc
    21001 gcggatatcc agcaacgtga aatcagatgt cttatctatg gaccagatgc cgcgcttggt
    21061 cttacgagca cgcgccacca ccgcgcggat cttttcggca aagattctgt ccgtggtcgt
    21121 gtaaaacgtt gggtaaacca acccttcacg cgcaagttga aaattgatcg aatcctcaat
    21181 gggcaggttt tctgaatcca tcacggcgcc gtcggtgagt tttgcatttt ttggaaagag
    21241 ataactcacg gggcgtccga agcggtctgt cgaagccgag gcgatgaaac ccgccttgcc
    21301 gtcgtctgca tccacgatct gggtcacgct caaactgtac gtcaccgatt tgatattgag
    21361 caattccaac agagattcca gggccgcaat cgcaaaggga cgcggctggt gatagccttc
    21421 gtaatgggtt tcaagtgcat cgatcgattc cacgcgcagt tgctttttct ttgccgcctt
    21481 atcggcttcc gtttcccatt caaaaaaatc ccaatgttcg ggtttgttcg cctgaaatcg
    21541 gatcgaatcg ccgtcgggtt gaaacccctt cacatgaaac gtacctgcaa ctactttgta
    21601 ttgtggcatg gtcatctcct tggttgatgg cctcattata gggaatttcc ccgccgaagg
    21661 caatcaactc acagtaaaat aaatacatgc ccactgtccg ctttcccgct ctgatgaaat
    21721 tttatgtcga taaccaaacc gagttcgaag tgcaaggcgc caccgttgcc gaagtgcttg
    21781 aaaacatcct cgtgcgttac cccgctttga aaccgcactt gtatgatgca aaaggcgaac
    21841 tgcgcaggca cttcaacatt ttcgtcaatg gggtgcattt gcgtgaattg aatggattgg
    21901 cgacggaatt gcaaacaggc gacaagatca ttctgatggc ctccgccgct ggaggatgaa
    21961 cgcttcacgc ccaacggaag cctgattcgg ttttctgaaa aagcgacctc cactcgggga
    22021 attttagtat tgacaaattg ggtaagatac gtaaaataac gcgcatctaa ctggaaacct
    22081 atggatacat tatttaacta ttgcttgtgt ctatcgcggg gctcgtaacc acccgcctga
    22141 gatagacccc tccgcagtca acggaaacag acggttggac gacccccgct tgcagaattt
    22201 tttgcatagt gtggcgctcg ttcgccgtct ttttgtttac ccgttgactg cttttgattt
    22261 aaaaggatga catggcctta ctggatattc gacttaccca atccaatacc attcccctcg
    22321 atagccttgc ctatcatgtg ggaaataccc ccctgctccc actgcggcgg gtgaccgcaa
    22381 aactctctcc acgtgtgaag gtgtttgcca aagccgagtg gttcaacccc ggcgggtcgg
    22441 tcaaagaccg ccctgctttg aacatcatcc agaatgccat catcaacggg aatttgagcg
    22501 gtggaaagcg cctgctcgat tccacctcag gcaatatggg gatttcctac gccacctttg
    22561 gcgcagcact tggaattcct gtcacccttg ccctgccagc cagcgccagc cccgagcgaa
    22621 tttccatcct caaagccctc ggcgcggaat tggtactcac cgacccgctc gaaggctcag
    22681 acggtgccat tctcaaagcg cgtgaaatgg ttgcggaaaa cccagacctg tattggtacg
    22741 ccaatcagta cagcaacgaa gccaattggc aggcccatta cctctctacc gggcctgaga
    22801 tcatcgccca aaccaacggg ctggtgactc atttcgtaac gggtctcggc acctctggca
    22861 cgttgatggg caccggacga tatttgcgcg aacaactgcc agatgtgaag attttggctt
    22921 tccagcctga tgcacccttc cacgggctgg aagggctgaa gcacatgcct agtgcgatca
    22981 aacccggtat ctatgacgag tcagttgcgg gcaacccgct tgaggtccag accgaagcgg
    23041 cgcacgagat ggtatcaagg cttgcacggg aggagggtct ctttgtgggg atctcttccg
    23101 gggcggcggc tgtcgctgcg cttcaagttg caagtgaatt ggacgaaggt gtggttgtaa
    23161 ccttgttccc cgatgcgggc tataaatatt tgagcgataa atctttgtgg gagggaaagg
    23221 ctggtggacg gtaggccgca ggttgtgtct atcgtcggca gttgtttcag gtgaacaatg
    23281 aatttgaaac ttacgaaaaa tattcttgat gatatcagca aacatgtgga ggccgcctac
    23341 cctgaagagg gggctggttt tttgctcggc gtggaaggtg aagtgaagca gattttatcc
    23401 ctgcccaatg cgcgcgagga tgaagcacgc cacaatcggt ttttgttcac ccccgaagat
    23461 tacctgaaag ccgagttgaa ggccgatagc ctcgggttga gcctgatcgg tgtttttcat
    23521 tcacaccccg attgcccgaa cattccctcc gagtatgacc gcgaatgggc gcagccgttc
    23581 ttctcctaca tcatcacacg ggtggatgaa ggccggtcgg tcagtcatcg ttcatggcgg
    23641 ttggtcgaag atcgttcgaa gtatgaagaa gaagagattt caataaaata aaaacaggat
    23701 accttaacat gacatcacaa accttacaaa aagtggatca ttctgcatta aaggccaacc
    23761 aatatttcat catttcgttg ggcgtgcttg cttttgtttt caaccagccg atccttgcgg
    23821 cattcatcgc gttgatgatg ggacttggca ccattttgaa gaaacccggc ttcggattta
    23881 tctacacatc cctgctcaaa ccacgcggat ggatgaagcc cgatgtgttg gacgataacc
    23941 ccgaaccgca tcggtttgcg caattcgtcg gatttttgtt catggctggc ggatcggccg
    24001 ctttgttcct cggttcaacg gtcctcggct ggaccctggt gtggatcgtc gttgccctgg
    24061 cctcgctcaa cgcctttgga ggtttttgtg tgggatgcgc catgtattac tggctggtgc
    24121 gcctgaacgt gcctggcttt gccaaacagc cgcccgcggg gaccttcccc ggcatgaagc
    24181 cgaaagcagg agccacagca tgagttcaga tatcctgttg cgttttgctt ttgcaattgg
    24241 tattatattt ttaggcgtgg gcgtcaactg gctgttcaat aagcggttga tctcacgggc
    24301 ccggaatggc atcgggtccc tgttcaatac gccgccgaac aagcccacca ttgtctattt
    24361 caccacgcca gattgtgcgc cctgcaaaac cgtgcagcgc cccgccctcg aaaagctgac
    24421 cagcctgttc ggtgaaaaac tgcacgtcgt tgaaattgac gcaacccagc agcccgaggt
    24481 tgcaaagacc tggggtgtca tgagtgtgcc gaccacattc ctgctcgatt cccgtggtga
    24541 ggcacgttat gtcaataacg gtgtggcgcg ggttgaaaag ttgatggaac agatacagac
    24601 tttgtaaatc atttaccacg aaggtcacaa agtgtcacaa aggaaaacct taagtccgtg
    24661 tttccttcgt gtacttcgtg ccatttgtgt ttaagttttt tcaaaattat tttaggagac
    24721 tctacatgcc cgttttgcga atcccgaccc cgttgcgtgc ttataccagc ggacaagccg
    24781 aagtgacagt caatggctca aatatttccg aagccctgac cgatctgacc aatcaatttc
    24841 cgacgatcaa gccgcatttg ttcaacgatg gcggggaact gcgtccgttt gtcaatttgt
    24901 tcattggcga acataacatc aaggatttgc agggcgtgaa tacgccgatc aaggatggcg
    24961 ataaggtaat gttgattccg tccattgc
//
LOCUS       DAOQLC010000062        24976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_662_length_24976_cov_26.776815, whole genome shotgun sequence.
ACCESSION   DAOQLC010000062 DAOQLC010000000
VERSION     DAOQLC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_24976_cov_26.776815"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1510)
                     /locus_tag="PL000_12075"
     CDS             complement(107..1510)
                     /locus_tag="PL000_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89683.1"
                     /translation="MNRKVFTLLLVAVFALAQFSVASAVQVTADGTLTAPDFMPAGAG
                     ATVTLDWTATGLPAGTTYIDIYARLQGGAYVVGECQNVAVIVGSNNSSGTFDYVFTGA
                     PADGEYWEFLIAFDDAATCTTLPALPADADNFMASSYVDGNKLDGYGFFPPFDGVVVT
                     TPVACNTFEYWAIASDNYNIPITEPYSGIGSWNPQVTGTFAPAPVGADDEVFSWVFTL
                     PSTASGLWQFSIDPEDAAGNVNGGGPFYFRNSALTTAVETADCETFTDIAGHENEVYV
                     RYLATLGLISGFADGTFGPDTTLTRAEAATLIEISNGYDDTTLPAAAPAGCDFTDVAA
                     TDWFAGWVWQACDDGYMNGVGGGLFDPNNLLTRGQIVTILNNIAESTTPPSGGFLAIG
                     PMLYPMFNGLPFQMRESAWSDVSIGAYYAEPVVEAYGWGIADGTSETTFSPDQPVTRG
                     EFAKMLYRALSLIFFAF"
     gene            complement(1871..3256)
                     /locus_tag="PL000_12080"
     CDS             complement(1871..3256)
                     /locus_tag="PL000_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89684.1"
                     /translation="MKTRNQTDDKLGEILEACLSDIKAGRATREECLSRYKDHARELE
                     PLLRVATSLSTHPKPRPSEKRVFTSRQRILQEFHSKERIPWFSGLRRSFTAPALAFST
                     VLTFIIMSFFGARVYFSTVTDEPTASAYPNLGPVITQTVPPQSEPTIAFNGIGLPGEQ
                     DGPYEIASAPDGTLWFARTDATRVGSVSAEDGKVQDWPLAANEGRAAGITVDKNGRVW
                     FTVSSSQSVIALDPATNEVSSWRVPPGDGTLGRIEAGEDGHIWFIARDGSRIYALDPK
                     TDNFTAYDVNGAEYIEWADDSLWFSGKGELGRIAPDGQVQVYDAPGDPAALVWDGSAL
                     WYAGGSLIGRFDAATDQVVVYPLENKTADRIATDGLKNIWFGSQGGTELGVLQSDSGQ
                     ISTRSMSAVVQNLYWLAVGTDQVWMSDGDSQFVYSFSLTKSGEVSFSVPINTQDQLAV
                     SIPLAALPAFH"
     gene            complement(3259..3831)
                     /locus_tag="PL000_12085"
     CDS             complement(3259..3831)
                     /locus_tag="PL000_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC89685.1"
                     /translation="MNNEASHISQGSAPPETDIPNLVQRIQDGDSQAVAALYSMHYQQ
                     LYTFAYYRLEGNVPLAEDITEEAFLRAFKTIGTFKWKGVSFGAWLVTIARNLIVDMLR
                     RPDTLQLQETWLDATSDPEKLVEQTLTNKELGVALEKLTSEQKEVIILRFFDDFSIAE
                     TAKILGKSIDAIKRLQARALVVLESNLSRD"
     gene            complement(3944..4810)
                     /gene="rfbD"
                     /locus_tag="PL000_12090"
     CDS             complement(3944..4810)
                     /gene="rfbD"
                     /locus_tag="PL000_12090"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNC89686.1"
                     /translation="MKILLLGNTGQLGWELQRCLPPLGDVRGLDYPQIDLANPETLRD
                     VIRTYKPDTIVNATAYTAVDVAESKSELAFAINAKATAVMAEEAKLSNAVMVHYSTDY
                     VFDGAKGSAYTEADATNPLNVYGASKLAGEQVVQAAGGAHLIFRTAWVYSLRRDSFVT
                     KTLQWARQHETLRLVDDQISNPTWARLLAEVTAQILARGPGYLCERTGLYHLAGDGYA
                     SRLEWARLILELDPNRHEQKVKEILPASTSDFPAPAMRPLFSALNCDRFTATFGLRLP
                     HWESALRMAMDR"
     gene            complement(4811..5683)
                     /gene="rfbA"
                     /locus_tag="PL000_12095"
     CDS             complement(4811..5683)
                     /gene="rfbA"
                     /locus_tag="PL000_12095"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HNC89687.1"
                     /translation="MKGIILAGGRGTRLHPLTFSMSKQLLPVYDKPMVYYPLSMLMLA
                     EIRDILVISTPEDLPLFRNLLGDGTQWGLKFSYAEQDQPRGLADAFRVGAKFVGNDSS
                     CLILGDNIFFGHGLPEILRRSATLKSGARIFAYPVRDPERYGVVEFDKDGKVLSLEEK
                     PAQPKSNYAVPGVYFYDPGVIEMAANLQPSKRGELEITDLNRLYLERGDLRVEALGRG
                     VAWLDAGTHESLLQASNFIQAVQERQGMMISCPEEIAYRMGFIDKKQLQELAGRLKNN
                     SYGQYLFDLANGKI"
     gene            complement(5680..6759)
                     /gene="rfbB"
                     /locus_tag="PL000_12100"
     CDS             complement(5680..6759)
                     /gene="rfbB"
                     /locus_tag="PL000_12100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNC89688.1"
                     /translation="MKNVLVTGGAGFIGSNFVRYLLEADERVHIVNLDALTYAGSLEN
                     LKDLPHAERHTFVQGDICNRDLVRDLLHQHQIDTVIHFAAESHVDRSILSPEPFIQAN
                     VFGTFILLEAARQYWQVEKALPLDEVRFHHVSTDEVYGTLAPDDPAFSETTPYAPNSP
                     YAASKASSDHLVRSYNHTYQLPVTISNCSNNYGPRQFPEKLIPLVILNALTGKQLPVY
                     GDGQQIRDWLFVTDHCEAIHRIVVDGKPGETYNIGGNNQPANLTIVETVCDLLDELQP
                     LAESHRSLIKFVADRPGHDRRYAMNISKIERDLGWKPRHSLTEGLLATVQWILANPDW
                     VSAIQKQREYQTWLAKNYEKREKPS"
     gene            complement(6775..7323)
                     /gene="rfbC"
                     /locus_tag="PL000_12105"
     CDS             complement(6775..7323)
                     /gene="rfbC"
                     /locus_tag="PL000_12105"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNC89689.1"
                     /translation="MQFIPTSLPEVILVVPKVYEDERGFFLESYQKERFAAAGISFDF
                     VQDNHSASVQGVLRGLHYQIRQPQGKLVRAVVGEIYDVAVDIRRSSPTFGKWVGAVLS
                     SENRNQLWVPPGFAHGFYVMSGRAEILYKATDYYAPQWERSLLWNDPALGIDWPVTGE
                     NFPILSGKDAQGVLLKDAEIYD"
     gene            complement(7331..8098)
                     /locus_tag="PL000_12110"
     CDS             complement(7331..8098)
                     /locus_tag="PL000_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89690.1"
                     /translation="MTNSNHMPEDVIRLEGVSVLYRKPGEKIRTFKEYAIRILQRRIQ
                     FKEFRALNDINLTIQKGEIFGVVGNNGAGKSTLLKVVSRVLIPTEGRVWIKGVVTPLL
                     ELGAGFHPELTGRENIMLNGTILGHPRREIEEHMDEIIEFSELGDFIDSPLRTYSSGM
                     YARLGFAAATTWEPDILLLDEVLSVGDESFRKKCEARMRGFRNGKTTSIMVSHSMESV
                     MNLCQRVAWLDHGTVRAVGPAVDIVAEYRKAQPGAGA"
     gene            complement(8103..8930)
                     /locus_tag="PL000_12115"
     CDS             complement(8103..8930)
                     /locus_tag="PL000_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89691.1"
                     /translation="MKTSEEFIYDSARRGPVALEELRGIFRYRDLILQLIRKDIVARY
                     KRSVLGVAWTMLQPLGMMIILSVVFSTLFSQVKGYPAYILSGLIAWTFFSQATSAMMT
                     QIVWGGALLRQIYLPRTSFAVSSIGTGLTNLGISFIPLVFIVLIIGLPIRWSILFVPI
                     SMLLLGAFALGVGLIVSTMSIYFPDVSDMYSLVLTGWMYLTPIVYPETLIPEAYRYWF
                     FHLNPMYYYVKIFRAPIFEGVIPDGSTLLVAALISTVTLAIGWLFFSKRSDEFAYHL"
     gene            complement(9067..11634)
                     /locus_tag="PL000_12120"
     CDS             complement(9067..11634)
                     /locus_tag="PL000_12120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89692.1"
                     /translation="MPINMLRWKNKSLTLAVYGLLISVGAFVTAWVVYQVPSDPKNNI
                     FLGLSLQRMILIGSVALAGLLASAISVRSFRDETWAEWTWTFLFGRSFLAGLIRWLAA
                     GIAITGWVIWFTPLYRWESNQDYYLRLHPIIVWLTLAGVLTFVVSGVEEYGFDPQNFL
                     STLSSNKKILGIALGVMFAFLLVWAFIALTGMGVWVSDGFWYGAGVPVLSLQIVLAMV
                     IGLGVFALERSPFKARFPKWTDLFIFFLIWGVAAYLWAREPLPTSFFAPGPFLPDYEL
                     HPFSDAATFDRGSQFALIGQGINNGAYWDRALYMAFLVFMHQLFGQNYEHLMNVQAAI
                     YAVFPALLYLLGRDVFNRSFGVILAALVTMRGVNGIMVSTLIDLANQKHMLTDFPTVI
                     FVAWFALMMVRWLKDPARNYLNAVWAGGVVGLGIMLRTNTLFLLVFAGILVPFVYWRQ
                     KLRGAVIGVLILVAMFASTYAWGAYHDKSVFDIYFYRIMIVLKARYPQAAPQLAPEEE
                     PQGNEASPLHVSTVSSTGPRLAEPARVKPVYEFVTIHFLHNIISSVFILPTDDEFHDL
                     WRTVRGDFPYWEPYWDGRFAESAHRFLTLNLLLVALGIAAAWKYSRAPGLAPLGIFFF
                     YDLANAFARTSGGRYVVPIDWIILFYFALGLFQVILWGMAFFGLREETAPVAEQVSWS
                     WEPLKKKAPLIVIFFLFAGSLVPVSGHFIPRRYPERSQEELFALLEAQGDIQKMGYDA
                     AELQAAFEQWPELRIVAGRALYPRYFEAGRGIPKNRFPYTAMDFPRIAFTVIGPQGLS
                     YVLLPRADVDYFPNASDVFVLGCGLGANIDALAVVIVDQPNIVYTRRPPVPLQCPVQ"
     gene            11686..12510
                     /locus_tag="PL000_12125"
     CDS             11686..12510
                     /locus_tag="PL000_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89693.1"
                     /translation="MPKTSSKKPIGNPTRGKTATNRLRRVDNFILLYEPALLTRTDGL
                     FQHSMFIDLGYGFDARTTLESAERFRRINPSLPILGVEIDKERVEAALPFADSITHFR
                     HGGFNLPLQSGERVRLIRAFNVLRQYEEKDFAPAYERLAEYVLPGGLMIEGTSNPFGS
                     IWVANLVRKMESGWKFEALVFSTNFHMGFDITDFQAVLPKNHIHHVVPGETIYDFFEA
                     WKRSAAETAHAKVFGWKQWFIASAESLAAKGFKIDVHKKWLAKGWLILKNGTSAED"
     gene            12752..13663
                     /locus_tag="PL000_12130"
     CDS             12752..13663
                     /locus_tag="PL000_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89694.1"
                     /translation="MASFKKIRLLAVVLLLISMACATPSIFPSNESEINTAVAGTVMA
                     GQTQDAVNELLTPPTIQDTPTLVPTLTFTPEPPTATPTPTVVPTLPFTPTAETPFITV
                     SVDTNCRSGPGKVYDVVGALLVGEFAEVFGRDPSNNYWYILNPDGNDPEFCWVWGKYA
                     TLTGPAMLAPVFTPPPTPTATATSLPTPKFTVEYTSMDSCNGFWWVELKLKNTGGIPF
                     KSVNISIRDKVTDVVVSDTADGFINLDDCQTKSAKEILGIGETFTISTPAFKYNPESH
                     RLFVTITLCSDKGQQGTCATEKIDFEP"
     gene            13680..14495
                     /locus_tag="PL000_12135"
     CDS             13680..14495
                     /locus_tag="PL000_12135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC89695.1"
                     /translation="MSRSTQAADLNRQFQNKTRTRIRAPKTLFFLLAVMLLVSCTSSP
                     EPAAPTLTFKNTVVSLTFDDGDADNYAVRDILARNNLRATFYVVSGFTGTNGYMTEDQ
                     LRDLYNDGNEIGGHTLNHSNVPDLSGADLEREICQDRKNLLAHGFEVNSFAYPYGHYD
                     DESKQMVVDCGYSNARGVSGGPDTIPPVDMYALNAMPYIVKDTRFPKMTRYVTEVEND
                     GGGWVIFVFHHVCDGCNEYAIKLSVFDQFAKWLGEQQANGLVVKTMSEVANPK"
     gene            complement(14563..14982)
                     /locus_tag="PL000_12140"
     CDS             complement(14563..14982)
                     /locus_tag="PL000_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89696.1"
                     /translation="MKLNSAKEVWVARLIAWVMIGAVSCGIIFVTTFMLGPSMFAVPD
                     LSEKIAVAVFCPGAEGNSRQDGASVPTTSSPSGTYGHTVEITCTFADGSTKIIRNEQY
                     ALASIGGMFGLGGLCGVAISIPLMLLPLFLIRRKKAE"
     gene            15428..16006
                     /locus_tag="PL000_12145"
     CDS             15428..16006
                     /locus_tag="PL000_12145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC89697.1"
                     /translation="MLDIQTQLFEATDIRFGPIDHETHPEIESKWTHDAEFMRLLDLK
                     PVRPLSPAMIKKQYEAIEKEIEEDKNLFYFTIRAREDDHLIGKAMVEWIDWTNGNGFI
                     RLGIGAAEDRRKGYGSQALHMLLRYAFGELNLYRVTAVVPAYNEGALRLFQKFGFVEE
                     VRRRQALNRDGQFWDLISFGLLNTEWQDQARA"
     gene            16016..16573
                     /locus_tag="PL000_12150"
     CDS             16016..16573
                     /locus_tag="PL000_12150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC89698.1"
                     /translation="MKDIYRGTLVRLCDESPEIMAKAFAKWDRDTEQHRLADSDPAML
                     LSEKKIKEIVEKRAENKQSAPFSIRTLAEDKLIGGVGLWVHSWSHSDAWLGISIGDRE
                     YWGKGYGTDAMRLMVQYGFIELNLRRISLGLHAYNERARKSYEKIGFKMEGRTRGDGL
                     RDGVRFDSLWMGILREEWLAAQELA"
     gene            16570..17130
                     /locus_tag="PL000_12155"
     CDS             16570..17130
                     /locus_tag="PL000_12155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNC89699.1"
                     /translation="MKDILKGNTVRLSAMNHEEIGQAMSAQARDSELMRLMSTSPSRL
                     HSAKTSVKFFEKMIEKNSPAEYFFSIRALEDDRLLGDINLDVINNWSSRDSFVGLGIY
                     DRADWGKGYGTDAMQITLRFAFTELNLHRVTLTVFEYNPRAIRSYEKVGFKHEGRIRG
                     GLIKDGKRWDMFFMGILHNDWKELQK"
     gene            17127..18119
                     /locus_tag="PL000_12160"
     CDS             17127..18119
                     /locus_tag="PL000_12160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89700.1"
                     /translation="MTQPNLRETLLDTTLKLRTAQWADCEAVAGLIYAVCEADGDTTV
                     AVTPEELKNEWQGEGFNLETDAFVVTTHDDRIIGYEEFFNQHEHYNLRTDGYVHPQFK
                     NLGIGTTLLRRVEARAREEMQLAEPDLRAFLRSTIDSRDAASHELHKAEGYSPKRYHW
                     RMEIKLESAPSPAVFPAGVELRPFIRAEHEVAVWQAQNEAFRDHWGSHDVSFEEFTRS
                     RFDDPEFDPSLWAIAWDGNEVAGFSLNRFRMGIGWVRTLGVRRPWRKKGLGLALLQYS
                     FGELYKRGKDTIGLGVDASSPTGATRLYQRAGMRIASEFLTYEKELRPGRVVED"
     gene            18315..18737
                     /locus_tag="PL000_12165"
     CDS             18315..18737
                     /locus_tag="PL000_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89701.1"
                     /translation="MNLRCIYCQTPFTLSRPEMLTAIVTMNEKNLNHYDSHCPRCRRA
                     NSISRQRMELFFPNWQDAAKKQASADAKAAASAASAGVSATAYLKTEPLPKTAKPTAE
                     KKPAAKAAAKKPAAGKSSTAKAKAPAAKKSASTKTKKK"
     gene            18741..19340
                     /locus_tag="PL000_12170"
     CDS             18741..19340
                     /locus_tag="PL000_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC89702.1"
                     /translation="MAIRAVFFDFGGVIMRTEYQSPRQHLAERFGMDYDDIDKVVFAS
                     ESARRASVGAITEDAHWAEVLKRLKHPASEMKSFKDEFFGGDVIDRNLVEFIRSLRGQ
                     VHTGLISNAWSGLREFITSEKVITAFDSVTISAEVGVVKPEGRIYQVALDQAKVKAEE
                     AVFVDDMNINLEGCEDLGMTGILFKNPQEAIEELKRLLA"
     gene            19515..20765
                     /locus_tag="PL000_12175"
     CDS             19515..20765
                     /locus_tag="PL000_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC89703.1"
                     /translation="MFINLKLDAKKVYLFIEFTASALFAMMFVTMSLYEATVAGLTPL
                     QLVLVGTALEVSAFVFEIPTGVVADVYSRRLSIIIGYFLMGIGFLVEGLFPAFLPILL
                     AQVIWGLGYTFTSGATQAWISDEIGEEEANKLVLRANRVGLYASLLGMGMAVLIGSQS
                     VGLPILFGGAGVVLIGLVLVVIMPETGFHPTPREDRNNWQHMAHTFKEGIKAVRARPR
                     LMTILGIGLLYGLYSEGFDRLWVKHILDTFELPVLFGQTDVAFFGLLRAGSMVLSIFA
                     TRFVEKRIDTGSPLAIGRATLGITFGISAGLIGFALSPIFAVTIIVYWFISVLRNVAG
                     PLYNTWVNQKLDPNTRATVLSMSSQVDAIGQMGGGPLAAAVAGTVSVVAAIITSGLLL
                     LPALPLIGRANSQSIAEKVEQTVS"
     gene            20881..21798
                     /locus_tag="PL000_12180"
     CDS             20881..21798
                     /locus_tag="PL000_12180"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNC89704.1"
                     /translation="MDLSKHAFFEGRIVPLADAKINIATHGFLYGTAVFSGMRAYWNE
                     EKKRLFVFRPFDHYQRLLNSARMMAMDIPYDEESLIQLTLDLLRTDNWQQDVYLRPTI
                     YKADMGIGVRLHNLKDEFSMFVMAFEKYVPNDANAHITFSSWRRIDDNSIPARGKIAG
                     AYANSALIKTDANRAGFDEALVLDNNGHVSEGSAMNIFIVRDGVLITPPVTDNILEGI
                     TRRSVIELARNELGLQVQERSIDRTEVFIAEEVFMTGTAAQIVAVTKIDHRPVGIGKM
                     GPITEKLRGLFDEVVRAKLPKYRQWNLEV"
     gene            complement(21877..22290)
                     /locus_tag="PL000_12185"
     CDS             complement(21877..22290)
                     /locus_tag="PL000_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89705.1"
                     /translation="MFQVFTGRAALRNIFLALFLLSTIAFVVMAVSTSLVKEAVSEFA
                     PSATTGDDPSSTPPSQDANGMGITAVIGSVVTSVTSLVGFIITTVITWRKEQREASLA
                     EVERKKLELELEKNRLELEELKKGEGKKKEARRKK"
     gene            complement(22309..23043)
                     /locus_tag="PL000_12190"
     CDS             complement(22309..23043)
                     /locus_tag="PL000_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89706.1"
                     /translation="MSKYDYNYEATSNPKQSAKLEVWDMLTIATLLLTLCLGVYFVAI
                     FIFPQSAINPLKASMVDPNAPPTATITPLQLDATWTATPFSIVTETPTLLPTHTLIPS
                     PTLFSLITPSDTPTPTPIKAQFSATVTYIDSTIIHPEADCKWQGIGGTIVDANNADIL
                     RITVRLVGSYNGSTKNELTVSSIAPAYGKSGYEFFLGTTPITSRDNLTLQILDQAGLP
                     LSDPIEIDTFKECSKNLVLVRFKKNP"
     gene            complement(23096..23983)
                     /locus_tag="PL000_12195"
     CDS             complement(23096..23983)
                     /locus_tag="PL000_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC89707.1"
                     /translation="MFSKPRFFAQLTLAFVLGLSACASFGTSNTPTPELPTSTPLPPT
                     ATPPPSVATVNGEYITTAEFQAELERYKSAQTALGLSFTEEDAAKAVLEDMISQVLLA
                     QAARAANFTLTEADLQSRIDALAAQLGSADALSQWQSTHGYDDASFRFALTRSIEAAW
                     MRDKIIADVPTVVEQIHVRQILTYNEADARAALDEIKAGADFDEKAAFFDPITFGELG
                     WVPRGYLLDPAADEALFALQPGAYSDVIATDAGFHIFKVVERGDHPLTPDALLVVQER
                     AVINWLADQRAKSDVTLIP"
     gene            complement(24056..>24976)
                     /gene="groEL"
                     /locus_tag="PL000_12200"
     CDS             complement(24056..>24976)
                     /gene="groEL"
                     /locus_tag="PL000_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNC89708.1"
                     /translation="VIIAEDVDGEALATLILNKIRGMLNVLAIKAPGFGDRRKAMLQD
                     IASLTGGQVISEETGRKLESTTVQDLGRAEKVVSDKENTTIVGGKGKKGDIEARIKEI
                     RIEMDKSTSDYDKEKLQERLAKLSGGVAIIRVGAATETEMKEKKHRVEDALSAARAAV
                     EEGIVPGGEISLINAAGKLDKLAKAHAEDENEEIKVGINIVKKALEAPIRKLSSNAGQ
                     DGSVIIDTVRRTAAEKKNPNIGYNVLTGEYTDMIKAGVIDPVKVVRGALENAASIAAM
                     ILTTDVLITDMPEKDKAPAMPPGGMGGMDY"
BASE COUNT         6365 a         6665 c         6471 g         5475 t
ORIGIN      
        1 caaaaaacag gcttttttgc catttttttc gaaccctcac ctgacaaaaa agagagcgga
       61 cccgatctgg gtccgctcaa gttgttaccg ttccaaatgg aattttttag aaggcgaaga
      121 agatcaagct caaggctcgg taaagcatct tggcaaattc gccacgggtt acaggttggt
      181 ctggtgagaa ggtggtctca ctggtgccat cggcaatacc ccacccatac gcctcgacca
      241 caggttcggc ataatacgca ccaatggata catcactcca tgcagattcg cgcatctgga
      301 aaggaaggcc attaaacatg gggtaaagca tggggccaat agccaggaag ccgcccgaag
      361 gaggtgtggt tgattctgca atgttgttga ggatggtgac gatctggccg cgggtgagca
      421 ggttgttcgg gtcgaacaag ccgccgccca caccattcat ataaccatca tcacaagcct
      481 gccagaccca gccagcgaac cagtcggtgg cggctacatc agtgaagtca caaccagcag
      541 gagcagcggc gggcagggtg gtgtcatcat acccattgga gatctcaatg agggtcgctg
      601 cctcggcgcg ggtgagggtg gtatcggggc caaaagtgcc atcggcaaag ccgctgatca
      661 ggccaagggt ggccagatag cgtacgtaca cttcattctc atggccagca atgtcagtga
      721 aggtttcgca atcggcggtt tcaacagccg tagtaagagc gctattgcgg aagtagaagg
      781 gaccgccgcc gttcacgtta ccagccgcat cctcagggtc gatcgagaat tgccacaacc
      841 cggaagctgt ggaaggcagg gtgaagaccc atgagaacac ttcgtcatct gcacccactg
      901 gggcgggagc aaaagtaccg gtcacctgtg gattccaact gccaatgcca gaatacggct
      961 cggtaatggg gatgttgtaa ttgtcagaag caatggccca gtattcaaag gtattgcaag
     1021 ccacgggcgt ggttacaacg acaccatcaa atggggggaa gaatccatag ccatcgagct
     1081 tgttgccgtc cacataagag gatgccatga agttatcagc atcagcagga agggcgggca
     1141 aagtggtgca ggtggccgca tcgtcaaacg caatgaggaa ctcccaatat tcgccatcag
     1201 cgggggcgcc agtgaaaacg taatcaaagg tgccgctgct attgttggag ccaacgatca
     1261 cggccacgtt ctggcattca cctactacgt acgcgccacc ctgtaaacgg gcataaatgt
     1321 caatataggt ggtaccagca ggcaggcctg tggcggtcca atccaatgta acggtggcac
     1381 cagcgcctgc aggcataaaa tcgggggcgg tcagggtgcc atcagcggtt acctgcactg
     1441 cggatgcaac gctaaattga gccagtgcaa atacggcaac cagtaacaaa gtaaatactt
     1501 tcctgttcat atatctctcc ttatcgatct gagaaatcat ttattgatta aatcattaag
     1561 tatgggcatt tatgtaatgt atcatctcct ttcaggaaag gtcatacaac ttaattaccc
     1621 catctctctt tcagtaacgc aataaaaaaa atcacgttac attaattaaa cggatgaact
     1681 ccgaaaaaga gaccggcaaa cccgaccaat tcctaaattg cccccacgcc tcaaatttga
     1741 cctgtcatct gctctgccca ccctcaaatt gcccaccctt gggcaggaaa cccgggattt
     1801 taagctaaaa aaatcccccg aactgagtcg ttcgggggaa atattaaaaa atgaatgttt
     1861 atgctggagg ttaatgaaat gccggcaatg cagcaagcgg gatagacaca gcaagctgat
     1921 cctgggtgtt gatgggtaca gagaaactta cctcaccgga tttcgtcagg ctgaaggaat
     1981 aaacgaactg actgtcgcca tcagacatcc agacctggtc agtacctacg gcaagccagt
     2041 acagattctg aaccacggca ctcatggagc gggtgctgat ctgtccgcta tctgattgca
     2101 agactcccaa ttccgtacca ccctgtgaac cgaaccagat gtttttcaag ccgtctgtgg
     2161 caattcggtc agcggtcttg ttttcaagcg gatatacgac cacctgatcg gtagccgcat
     2221 cgaaacggcc aatcaggctg ccaccagcat accaaagcgc ggacccgtcc caaaccagcg
     2281 ctgcggggtc acctggggca tcatatacct gaacctgacc atctggcgcg atccgtccca
     2341 gttcgccctt gccgctgaac caaagcgagt catctgccca ttcgatatat tcggcaccat
     2401 taacatcata ggcggtgaaa ttgtctgtct ttggatcaag cgcgtaaatg cggctgccat
     2461 ctctggcgat gaaccaaata tgtccatctt cgccggcttc aattctgccc aaggtgccgt
     2521 ctcccggcgg cacacgccag gaagagactt catttgtcgc cgggtccaat gcaatgaccg
     2581 attgagagga ggaaaccgtg aaccaaactc tgccattctt atcaacggta atccctgcgg
     2641 cacggccttc attagcggcg aggggccagt cctgcacctt gccatcttcg gcgcttacag
     2701 accccacacg ggtggcatct gtacgggcaa accacaaggt gccatcaggg gcacttgcga
     2761 tctcataagg gccatcctgt tcaccgggca ggccgatacc attgaaggca atggtgggct
     2821 cagattgggg agggactgtt tgagtgatta caggacccag gtttgggtat gcactggcag
     2881 ttggttcatc cgtaacggtt gaaaaataaa cccgcgcccc aaaaaacgac atgatgatga
     2941 aggtaagcac cgttgaaaac gcaagagcgg gagcggtaaa ggagcggcgc aacccggaaa
     3001 accaggggat acgctccttg gaatgaaatt cctgaaggat gcgttgacgg ctggtaaaca
     3061 cacgtttctc agaaggtcgc ggcttggggt gggtggacaa ggatgtggca acgcgcaata
     3121 aaggctccag ctcgcgggca tggtccttat agcgtgacag gcattcctcc cgggtggcac
     3181 ggcctgcttt tatgtcagac aggcatgctt ccaggatctc gcccagttta tcgtctgttt
     3241 gattccttgt cttcataatt agtctctcga cagattactt tccagtacga ccaatgcacg
     3301 tgcctgaagg cgtttaatgg catcgatcga tttacctaag atttttgcag tttctgcgat
     3361 gctaaaatca tcaaaaaagc gcagaataat cacttctttt tgttcggatg tcagcttttc
     3421 gagcgctacg cccagttctt tgttcgtgag ggtctgctca acaagctttt ccgggtcaga
     3481 ggtggcatcc agccaggttt cctgtaattg aagggtatct gggcggcgca acatatctac
     3541 gatcaggtta cgggcaatgg tgaccagcca ggccccaaaa gaaacaccct tccacttgaa
     3601 ggtgccaatg gtcttgaagg cacgcaaaaa cgcttcctcg gtgatatcct cagcaagcgg
     3661 aacattccct tccaggcgat agtatgcaaa tgtatagagt tgctggtaat gcatgctata
     3721 cagagctgct acagcctgtg aatcaccatc ctggatacgc tgtaccaggt tgggaatgtc
     3781 agtttcaggt ggtgcggaac cctgagaaat atgtgatgct tcattattca taacgtatga
     3841 aatcgaaaaa agagaccggc aaatcgaaaa ttggtcgaaa attgcaaaac tgcaaggtta
     3901 attaaggccc caaagagcag tgggagggag aaaaacacgg aatttaccgg tccatggcca
     3961 tgcgcaaggc agattcccag tgcgggaggc gcaggccaaa agtggcggta aagcggtcac
     4021 agttcaacgc cgagaacagc ggacgcattg ccggggctgg aaaatcagag gtggaggcag
     4081 gcaaaatttc ctttaccttc tgttcgtggc ggttgggatc aagctcaagg atcagacggg
     4141 cccactcgag gcggctggca tacccatccc ctgccagatg atacagaccc gtccgctcac
     4201 agaggtaacc gggaccacgc gctaaaatct gcgcggtgac ttcggccagc aggcgcgccc
     4261 aggtggggtt gctgatctgg tcatccacca gccgcagggt ttcatgttgg cgggcccatt
     4321 gcagggtctt ggtgacaaaa ctgtcacggc gcaggctata aacccaggct gtacggaaaa
     4381 tgagatgcgc gccgcctgcc gcctgaacga cctgctcccc tgccagtttg ctggctccgt
     4441 aaacgttcag tggattggtg gcatcggctt cggtgtaggc tgagcctttt gctccatcga
     4501 acacataatc ggtggaatag tgaaccatca ccgcgttcga cagtttggct tcttccgcca
     4561 tgaccgcggt ggctttcgcg ttgatcgcaa atgccagttc gcttttgctt tcggccacat
     4621 ccaccgcggt gtaggcggtg gcattgacaa tggtatccgg tttgtaggtg cgaatcacat
     4681 cgcgcagagt ctctggattt gccagatcaa tttgcgggta gtccagaccg cgtacatcgc
     4741 ccagaggcgg taaacagcgt tgtaattccc atcccagttg accagtgttg cccagcagta
     4801 gtattttcat ctatattttt ccattggcga gatcgaaaag gtattgaccg taagagttgt
     4861 tcttgagcct gccagccaat tcctgaagtt gttttttgtc gatgaagccc atgcggtagg
     4921 cgatttcttc aggacaggag atcatcatac cctggcgttc ctgcacggct tgaatgaagt
     4981 tcgaggcttg cagcagcgac tcgtgggtgc cggcatcgag ccaggccacc ccgcggccaa
     5041 gtgcctccac gcgcaaatcg ccgcgttcga ggtaaagacg atttaggtcg gtgatctcca
     5101 gctcgccgcg tttcgagggc tgtaaattcg ccgccatttc gatcacaccg gggtcgtaaa
     5161 aatacacacc cggcacggca tagttcgatt ttggctgggc gggcttttct tccaggctca
     5221 acaccttgcc atctttatcg aattcaacca cgccgtaacg ttcggggtca cgcacagggt
     5281 aggcaaagat gcgtgcaccg gatttcaacg tggccgagcg gcgcagaatt tcaggcaaac
     5341 cgtgaccgaa gaaaatgtta tcgcccagga tcaggcagga tgaatcgttt ccgacaaatt
     5401 ttgcaccgac gcggaacgca tctgccaacc ctcgcggctg gtcctgttct gcgtaggaga
     5461 atttcagccc ccactgagtc ccgtcaccga gcagattgcg gaaaagcggc aggtcttcgg
     5521 gggtgctgat aacaagaata tcgcgaatct ccgccagcat caacatcgaa agcggataat
     5581 acaccatggg tttgtcgtac accggcagca attgcttgct catggaaaag gtcagcgggt
     5641 gcaggcgtgt accgcgtcca ccggcaagga tgattccctt catgatggct tctctctttt
     5701 ttcgtagttt tttgccagcc aggtctggta ttcacgctgt ttttgaatgg cactcaccca
     5761 atcggggttg gcaaggatcc actgcaccgt ggcgagcaga ccttcggtca aagaatggcg
     5821 cggcttccag ccgagatcgc gctcgatctt gctgatgttc atggcatagc ggcggtcgtg
     5881 accggggcgg tctgccacga atttgatcaa actgcgatgt gactcggcca ggggctgaag
     5941 ttcatccaac aggtcacaaa cggtttcgac gatggtcagg ttggcgggct ggttattgcc
     6001 gccaatgttg taggtttcac cgggtttgcc atccacaacg atcctgtgga tcgcttcgca
     6061 atggtcggtc acaaacagcc aatcgcggat ctgctgtcca tcaccataca cgggcaattg
     6121 cttgccggtc aacgcattga ggatgaccag cggaatcaat ttctcaggga actgacgcgg
     6181 gccataatta ttcgagcagt tggaaatggt caccggcagc tgataggtgt ggttgtacga
     6241 gcgtacaaga tgatcgctgg atgccttcga agcggcatag ggcgaattcg gcgcataggg
     6301 ggtcgtttca gaaaaagcag ggtcatcggg cgcaagcgta ccgtacactt catcggtgga
     6361 aacatgatga aagcggacct catcgagagg cagagccttc tccacctgcc aatactgtcg
     6421 tgccgcttca agcaggataa aggtgccaaa cacattcgcc tgaatgaacg gctccgggct
     6481 caaaatcgaa cggtcaacat gtgactcggc cgcaaaatgg atgacggtgt cgatctggtg
     6541 ctggtgaagc aaatcgcgca caagatcgcg gttacaaata tcgccctgca caaaagtatg
     6601 ccgctctgca tgcggaaggt ctttcaaatt ttcgaggctg cccgcgtagg tcaacgcatc
     6661 gagattgacg atatgaaccc gttcatccgc ctctaaaaga taacgtacaa aattggagcc
     6721 gataaaaccc gccccgccag tgaccaaaac atttttcatt ttgtctcccg gagtctaatc
     6781 ataaatctca gcgtctttga gaagcacgcc ctgcgcatcc ttcccggaca gaattgggaa
     6841 attctcacct gtcacgggcc aatcaatgcc aagagcagga tcattccaaa gcagactgcg
     6901 ttcccattgc ggagcgtaat aatctgtagc cttgtaaagg atttcagccc gcccgctcat
     6961 gacgtaaaac ccatgcgcga atccgggcgg aacccaaagc tggttcctgt tttctgacga
     7021 aaggaccgcc ccgacccatt ttccaaacgt cggggaactg cggcggatgt ccacggccac
     7081 gtcgtagatc tcaccaacga ccgcccgcac cagtttaccc tgaggctggc gaatctggta
     7141 atgcaatccg cgtaaaactc cctgcaccga agccgagtgg ttatcctgca caaaatcaaa
     7201 tgaaatgcct gccgccgcga aacgttcctt ctggtagctt tccaaaaaga agccgcgctc
     7261 atcttcatac actttcggca cgaccagaat cacctcaggc aggctggttg gaataaactg
     7321 catgtagatt ctacgctcct gcacccggct gtgccttgcg atattccgcc acaatatcca
     7381 cggcgggacc cactgcacga actgtaccgt gatccagcca ggcaacacgc tgacaaagat
     7441 tcatcaccga ttccatggaa tgtgaaacca tgatggaggt ggtctttcca ttgcggaaac
     7501 cgcgcatacg cgcctcacac ttcttgcgga aggattcgtc accaaccgaa agcacttcat
     7561 caagcaggag gatatccggt tcccaggtgg tggcggcggc aaacccgagg cgggcgtaca
     7621 tgccgctgga ataagtgcgc aacggcgaat cgataaaatc acccagttca gagaattcga
     7681 tgatctcatc catgtgctct tctatttcgc ggcgcggatg accgaggatg gtgccattca
     7741 gcatgatgtt ctcgcggccg gtcaactcag ggtgaaagcc cgcgcccaac tccaacagcg
     7801 gcgtgacaac ccctttgatc cacacccgtc cttcggtcgg gatcaagacc cgcgaaacaa
     7861 ccttgagcag ggtgcttttc ccggcgccgt tattgcccac cacaccgaaa atctcccctt
     7921 tttgaatggt caggtttata tcgttcagcg cgcgaaattc cttgaattga atccgccgct
     7981 gaagaatgcg gatggcatat tctttaaagg tgcggatctt ttcccccggc ttgcgataca
     8041 acacggagac cccttcaagg cggataacat cttctggcat gtgattcgaa ttggtcattg
     8101 gtttacaggt gataggcaaa ttcatcggag cgtttcgaga agaacagcca cccgatggca
     8161 agagttacag tggagatcaa tgcagctaca agcaaagtgc tcccatccgg gatcaccccc
     8221 tcaaagatcg gcgcgcggaa aattttcacg taatagtaca tggggttcaa atgaaagaac
     8281 cagtagcggt aggcttcagg gatcagggtt tcagggtaaa cgatgggcgt caaatacatc
     8341 cagccggtca acaccaggct gtacatatcc gatacatcag ggaagtaaat ggacatggtg
     8401 gagacgatca accccacacc cagggcaaac gcccccagca gcagcatcga gatcggcaca
     8461 aaaagaatgc tccaacgaat cggcaggcca atgatgagca cgataaacac aagcggaatg
     8521 aacgaaatgc ccaaattggt caaccctgtg ccgatcgaag aaacggcaaa agacgtgcgc
     8581 ggaagataga tctgccgcaa caaagcgccg ccccaaacga tctgggtcat catggcgctg
     8641 gtggcctgtg aaaaaaacgt ccacgcgatc aaaccgctga gaatataggc cgggtatccc
     8701 ttgacctgac tgaacaaggt cgaaaagacc accgacagaa tgatcatcat gccaaggggc
     8761 tggagcatcg tccaggcaac gcctaaaacc gagcgcttgt aacgtgccac gatgtccttg
     8821 cggatcaatt gcagaatcaa atcacgatat cgaaagatac cgcgcaattc ttccaacgcc
     8881 accggtccgc gccgggcgct atcataaata aactcttcag atgttttcat accactcaca
     8941 aaaggtaact aaattccaaa aaactgaccc aaaaccaggg ccagaacaaa aatccatggt
     9001 gtcgattttt gaacgggatt aaaacagcgt tttcgattct accatacgga aaaaccgcag
     9061 aaacggctac tgcacagggc attgcaaagg caccggcgga cggcgcgtgt acacaatgtt
     9121 cggctggtct acaatgacca cggccagtgc atcaatgttg gcccccaacc cacaccccaa
     9181 aacgaacaca tccgaagcat tcgggaaata atccacatcg gctcgcggca gcaacacata
     9241 actcaacccc tgcgggccga tcaccgtgaa ggcaattcgg gggaaatcca tggcggtgta
     9301 cgggaagcga ttcttcggaa tgcccctgcc cgcctcaaaa tatcgtggat acaaggcccg
     9361 cccggcgacg atcctgagct caggccattg ctcgaaagcc gcctgcaatt ccgccgcgtc
     9421 gtatcccatc ttttgaatat caccctgcgc ttcaagcagt gcgaacaatt cctcttgtga
     9481 acgctcgggg taacggcgcg gaatgaaatg acccgacacc ggcaccagcg accctgcaaa
     9541 caggaagaaa atgacgatca acggagcttt tttctttaac ggttcccaac tccacgaaac
     9601 ctgctcagcg acaggagcag tctcttcccg caggccaaag aaagccattc cccacaaaat
     9661 gacctgaaac aagcccagcg caaaatagaa caggatgatc caatcaatgg gcacgacgta
     9721 gcgtccgccg gaggtgcggg caaaggcatt ggcaaggtcg tagaaaaaga agatgcccaa
     9781 cggcgccagc cccggagcgc gggaatattt ccaggcggcg gcaatgccca gcgcgaccag
     9841 aagcaggttc aaggtcagga aacgatgcgc gctttcagcg aagcgtccat cccaatatgg
     9901 ctcccaatac ggaaagtccc cgcgcaccgt gcgccacagg tcgtggaact cgtcatcggt
     9961 tggcagaata aagaccgatg aaataatgtt atgcaggaaa tgaatggtga cgaattcata
    10021 aacaggtttc acccttgcag gttcagccag gcgcggaccc gtggacgata cagtggaaac
    10081 atgcagcggg cttgcttcat ttccctgcgg ttcttcttcg ggtgcaagct gcggcgccgc
    10141 ctgcggatac cgcgccttaa gaacgatcat gatcctgtaa aaataaatat cgaaaacaga
    10201 tttatcgtga tatgcccccc aggcatatgt gcttgcgaac atcgcgacca aaatcaacac
    10261 gccgatcact gcgccgcgca atttctgccg ccaatagacg aagggaacca agatccctgc
    10321 gaacaccaaa aggaaaagcg tgttcgtgcg cagcatgatt cccagcccca ctacaccgcc
    10381 tgcccacacg gcgttcaggt aatttctggc ggggtccttc agccagcgca ccatcatcaa
    10441 agcgaaccat gccacgaaaa tgaccgtcgg gaaatctgtg agcatgtgtt tctggttggc
    10501 gagatcgatc aaagtgctga ccatgatccc attcacaccg cgcatcgtca ccagtgcagc
    10561 aaggatcaca ccaaacgagc ggttgaacac atcccgtccc agcaaatata gaagcgccgg
    10621 gaataccgca tagatcgcgg cctgcacgtt catcaggtgt tcgtaattct gtccgaacaa
    10681 ttgatgcata aataccaaaa aggccatgta cagggctcgg tcccagtagg cgccgttgtt
    10741 gataccctga ccgatcaacg cgaactgact gccgcggtcg aaggttgccg catcggaaaa
    10801 tggatgcaat tcataatcag gcagaaacgg tcccggcgca aagaaactgg tgggcaatgg
    10861 ttcacgcgcc caaagatacg ccgccacgcc ccagatcaaa aagaaaatga aaaggtctgt
    10921 ccattttgga aaccgcgcct tgaacggcga acgttccagt gcgaacacac cgaggccgat
    10981 caccatcgcc agcacgatct gcaaactcag cacaggcaca cccgcgccat accaaaagcc
    11041 gtcgctcacc cataccccca tgccggtcaa cgcaatgaag gcccaaacca aaagaaaagc
    11101 gaacatcacg cccagcgcaa tgccaaggat ctttttattc gatgaaagcg tgctcaaaaa
    11161 gttctgcgga tcaaaaccat attcttccac ccctgaaacg acaaaagtca aaaccccggc
    11221 gagggtcagc caaacgatga tgggatgcag acgcaggtaa taatcctgat tgctttccca
    11281 acgatacaaa ggcgtgaacc aaatcaccca acctgtaatg gcgattcccg ccgccagcca
    11341 gcggatcagc cccgcaagaa acgaccgccc gaagagaaag gtccacgtcc actctgccca
    11401 ggtctcatcg cgaaacgagc ggaccgaaat cgccgacgcc aacagccctg ccagcgcaac
    11461 actgccgatc agaatcattc gttgcagcga cagccccaaa aaaatattgt tcttcgggtc
    11521 agacgggact tgatacacca cccaggccgt cacaaacgcg ccgaccgaga tcagcagtcc
    11581 atagaccgcc agggtgaggg atttgttttt ccagcgcaac atattaatcg gcatgagttt
    11641 accataacgg aaccccatca atttcgcccg tataattggg catgcatgcc gaaaacttca
    11701 agcaaaaaac cgattggaaa cccaacccgc ggaaaaaccg ccaccaaccg actccgccgt
    11761 gtcgataact ttatcctgct ctatgagcca gcgcttctca cacggaccga cggcctgttt
    11821 cagcattcga tgttcattga tcttggctac ggcttcgacg cccgcaccac cctcgaaagc
    11881 gccgaaaggt tcaggcgcat caacccgtcg ctgcccatcc tcggcgtgga gattgacaag
    11941 gagcgggtcg aggccgcgct gccctttgcc gacagcatca cccactttcg gcatggagga
    12001 tttaacttgc ctttgcaatc aggcgaaagg gtaaggctga tccgcgcgtt taatgtcctg
    12061 cgtcaatatg aagaaaagga ttttgcgcct gcgtatgaac gtctggctga atatgtgtta
    12121 ccgggcgggc tgatgatcga aggcacgtcg aatccatttg gcagcatttg ggtggcgaac
    12181 ctcgtacgca aaatggaaag tggatggaaa tttgaggcac tcgtgttcag caccaacttt
    12241 cacatggggt ttgacatcac cgattttcaa gccgtacttc cgaagaatca catccaccat
    12301 gttgtgcccg gtgaaaccat ttatgatttt ttcgaagcct ggaagcgctc cgcagctgag
    12361 accgcccatg caaaagtctt cgggtggaag caatggttca tcgcctcagc cgaaagcctg
    12421 gcagccaaag gattcaagat cgatgtgcat aaaaaatggc tggccaaagg ctggttgatc
    12481 ctgaaaaacg gaacaagcgc agaagattga gcgtcaggtt aacgcaccag acgggtagaa
    12541 ttctcttaat taatagaagc atcctctcgt catgcaattc tcgtgaaata aggtgtgacg
    12601 cattaaatca gtttttctct accgtacatt tgcaccgcga agcccgcaaa gggcgcgaag
    12661 aaatctttaa aacctggcga tcttggcgtg cttcgcggta aaaatcgaga aatgtacggc
    12721 agcaagatca gtttcaaaaa aggaaagtct catggcatct ttcaagaaaa tccgtttact
    12781 ggctgtggtg ctgttattga tcagcatggc ttgcgccacg ccatccattt ttccatcgaa
    12841 cgaaagtgag atcaacactg ccgtggcagg gacggtcatg gcgggccaga cccaggatgc
    12901 agtaaacgaa ctcctcaccc cgcccaccat acaagacacc cccacccttg tccctacgct
    12961 gacattcaca cccgaaccgc ccacagcaac gcccacaccc acggtggtgc caactttgcc
    13021 attcaccccc acagcagaga ctcctttcat caccgtgtcg gtggatacaa actgccgcag
    13081 cggcccaggg aaggtatatg atgtggtcgg cgccctgctg gtgggagaat ttgccgaagt
    13141 gttcggccgt gacccaagca acaattactg gtacattctc aaccccgatg gcaatgaccc
    13201 cgagttttgc tgggtatggg gaaaatatgc cacactgaca gggccagcca tgctcgcgcc
    13261 ggtcttcaca ccgccgccca ccccaaccgc cacagccaca tccctgccca cgccgaaatt
    13321 caccgtcgaa tacaccagca tggattcgtg caatggcttt tggtgggtgg aactcaaatt
    13381 gaagaacacc ggcggcattc cgttcaaatc ggtgaacatt tcgatcaggg ataaagtcac
    13441 tgatgtcgtt gtctccgaca ctgccgatgg atttatcaat ctcgatgact gccagacgaa
    13501 aagcgcaaag gaaatccttg gcattggcga aactttcacc atcagcacac ccgccttcaa
    13561 atacaaccct gaaagccaca gactcttcgt caccattaca ttgtgttcgg ataaaggtca
    13621 acagggaaca tgtgcaacag aaaagatcga ttttgagccg taattacaaa ggagatgaag
    13681 tgtcacgatc aacacaggca gcagacttga acaggcaatt ccaaaacaaa acgcggacca
    13741 ggatccgcgc tccaaagacg ctgttttttc tattggcagt gatgcttttg gtcagttgca
    13801 ccagcagccc cgaacctgcc gcgcccacgc tcactttcaa gaacaccgtg gtcagcctga
    13861 ctttcgatga cggtgacgcc gacaactacg ccgtacgcga catcctcgca aggaataacc
    13921 tgcgcgccac cttctacgtc gtcagtggat ttaccggcac gaacgggtac atgaccgaag
    13981 atcaactgcg cgacctgtat aacgatggca atgaaatcgg cggtcacacc ctcaaccata
    14041 gcaatgtccc agacctgagc ggtgcagacc tcgaacgcga gatctgtcag gatcggaaaa
    14101 acctgctcgc gcatgggttc gaggtgaatt ccttcgccta tccctacggc cactatgacg
    14161 atgaatcaaa gcaaatggtg gtggattgcg gctacagcaa tgcgcgcgga gtctccggcg
    14221 gcccggatac cattccgccc gtggatatgt atgccttgaa tgccatgcct tatattgtaa
    14281 aagacacccg ctttccaaaa atgacccgct acgtcaccga ggtggaaaac gatggcggcg
    14341 gctgggtcat attcgttttt catcacgtct gcgacgggtg caacgaatac gcgatcaaac
    14401 tcagcgtgtt cgaccagttt gcaaaatggc ttggcgaaca acaagccaac ggattggtgg
    14461 tcaaaacgat gagcgaagtg gcgaatccaa aataggccac actgtaacgt gaatttttca
    14521 aagtcggctt caaagtctgt ggattttgaa gccgacttcg tttcactccg cttttttcct
    14581 gcggatcagg aataacggca aaagcatcag cggaatggaa atcgccaccc cgcataaacc
    14641 acccaaccca aacatgccgc caatggaagc cagcgcgtat tgttcattgc gaatgatctt
    14701 cgtactgcca tcggcgaagg tacaagtgat ctccactgtg tgtccatacg tacccgatgg
    14761 gctggatgtg gtcggcacag atgcaccatc ctggcgtgag ttgccttccg cgcccgggca
    14821 aaagactgcc actgcgatct tctcacttag atcaggcaca gcgaacattg aaggtcccag
    14881 catgaatgtg gtgacaaaaa tgatgccgca cgaaaccgca ccgatcatca cccacgcgat
    14941 caaacgcgcc acccaaactt cctttgcaga attcaatttc atgactcacc tctaatcatt
    15001 tttcgcggaa acacacaatt aacaatgatt gtatcgaatc caaacagcag atacaacctc
    15061 ccaaagtttc actgccgcga aatttcacgg cgatcggccg cagtgataaa agtacaatat
    15121 ttcccaaaac ccttgactct attttcctcc gcgtgataaa ctcaccacgc ttcaaaaaag
    15181 catattccat gaaaggaggc gcacgcctaa tgataatgaa caagttttac acctttatcg
    15241 ctcccatcgg taacaccgat gtcgattctc gcagtgcgcc tgcccgcagt tagacaaact
    15301 ttccaagttg tcatgcggcc acggtgtcac tgctgacccg tggccgtttt tatttaatcg
    15361 cgtgaaaggc ggccatgggt ccatgagacc tgtggccgtt tttttgtttt ttggtttgga
    15421 ggaaaaaatg ttggatatac aaacacagtt attcgaagca acggatatcc gtttcgggcc
    15481 catcgaccat gaaacacatc ctgaaatcga atccaaatgg acgcatgacg ccgaattcat
    15541 gcgcctgttg gacctgaaac ccgtccgccc gctctcacct gccatgataa agaagcagta
    15601 tgaggccatc gaaaaagaaa tagaggagga taaaaacctc ttctacttca ccatccgcgc
    15661 ccgtgaagat gaccatttga tcgggaaggc catggtcgag tggatcgact ggacgaacgg
    15721 aaatggtttt attcgccttg gcattggcgc agccgaagac cgccgcaaag gctatggctc
    15781 gcaggcattg catatgctgt tacggtatgc ctttggtgag ttgaacctgt atcgtgttac
    15841 ggccgtcgtc cctgcttata acgaaggcgc actacgcctc ttccagaagt ttggttttgt
    15901 ggaggaagtc cgcaggcggc aggcattaaa ccgtgatggg caattttggg acctgatctc
    15961 tttcggcctg ctgaataccg aatggcagga tcaggcccgg gcataggagg acgcgatgaa
    16021 agacatctat cgcggcaccc tggtccgcct gtgcgatgaa tcgccggaga tcatggccaa
    16081 ggcattcgca aaatgggatc gcgacaccga acagcatcgt ctggcggata gcgatcccgc
    16141 catgctgctt tcggaaaaaa agatcaagga aatcgtcgaa aaacgcgccg agaacaagca
    16201 atcggctccg ttttccattc gcaccctggc tgaagacaag ttgatcggcg gggtggggct
    16261 atgggtacat tcatggtctc actccgatgc ctggctgggc atttccatcg gcgaccgtga
    16321 gtattggggc aaagggtatg gcactgacgc catgcggctc atggtgcaat atggattcat
    16381 tgagttgaat ctgcgccgca tttcgctcgg cctgcatgcc tacaatgaac gcgcccgaaa
    16441 atcctacgag aaaatcgggt ttaagatgga aggccgcaca cgtggcgacg gccttcgcga
    16501 cggagtgcgc ttcgacagtt tgtggatggg cattctgcgt gaagagtggc tggcagcaca
    16561 ggagttggca tgaaagacat tctcaaagga aatactgtcc gcctctctgc catgaaccac
    16621 gaagagatcg gtcaggccat gtccgcacaa gcacgcgact cggaactgat gcgtttgatg
    16681 agcaccagcc cgtcccgtct gcattctgcc aaaacctcgg tcaagttctt cgaaaagatg
    16741 atcgagaaaa attcgcccgc cgaatatttc ttcagcattc gcgcactgga agatgaccgt
    16801 cttttgggtg atatcaatct tgatgtcatc aacaactgga gctcacgcga ctcatttgtt
    16861 gggctgggca tctacgatcg cgcagactgg ggcaaaggtt atggaaccga tgccatgcag
    16921 atcacacttc gttttgcgtt caccgaactc aacctgcacc gcgtcaccct gaccgtattt
    16981 gaatacaacc cgcgtgcaat tcgttcctac gaaaaagtgg gcttcaaaca tgaaggccgc
    17041 atacgcggag gtcttataaa agatggaaag cgttgggata tgttcttcat gggcattctc
    17101 cacaacgact ggaaggagtt acaaaaatga cacaacctaa tttacgagaa acgcttcttg
    17161 atacgaccct gaaactgcgc actgctcaat gggcagactg cgaagctgtt gcaggactca
    17221 tctacgccgt gtgtgaagcc gatggcgaca ccactgtggc cgtcacacca gaagaattga
    17281 aaaacgaatg gcagggtgaa ggtttcaatc ttgaaaccga tgccttcgtt gtcacaactc
    17341 acgatgaccg tatcatcggt tacgaagaat tcttcaacca gcacgaacac tacaacctgc
    17401 gcaccgatgg atacgtccac ccgcaattta agaaccttgg catcggcacc acccttttac
    17461 gccgagtcga ggcgcgcgcc cgtgaagaaa tgcagcttgc cgaacctgac ctgcgggcct
    17521 ttctgcgcag caccatagac agccgcgatg ccgccagcca tgaactgcac aaagccgaag
    17581 gctattctcc caaacgctac cattggcgca tggagatcaa actcgagagc gccccgtctc
    17641 ctgccgtatt tcccgcaggt gtggagcttc gtccgttcat ccgcgccgaa catgaggtgg
    17701 cggtctggca ggctcagaac gaagccttcc gcgatcactg gggcagccac gatgtatcct
    17761 tcgaagaatt cacccgcagc cgcttcgatg accccgaatt tgatcccagc ctttgggcga
    17821 tcgcctggga tggaaatgaa gtggcgggct tttcacttaa ccgcttccgc atgggcattg
    17881 gctgggtccg cacattggga gtccgccgcc cgtggcgcaa aaaaggcctt ggccttgcgc
    17941 tgctccagta ttcatttggc gaactctaca aacgcggtaa agataccatt ggccttgggg
    18001 tggatgcctc cagccccact ggcgcaaccc gcttatacca acgggcaggc atgcgcatcg
    18061 ccagtgaatt cctcacgtac gaaaaggaac ttcgccccgg gcgcgttgtg gaggattaat
    18121 ttacactcaa aaaagaccct gatggcctaa aaacgagcca attggaggct tgaatagaca
    18181 aaaaaggcct tcagggtctg tagtttctgg ccgtttcact gccacaaaaa atggaaatga
    18241 aaagccggtt cgcccggcgg gtgaaatcag tgtagaatca aagcatattc gtgtttttgg
    18301 aaaggataat ccctatgaac ctgcgttgta tttactgcca gaccccattc acccttagcc
    18361 gccccgagat gctgactgcc attgtgacaa tgaatgaaaa gaacctgaat cactacgaca
    18421 gccattgccc gcgttgccgc cgcgccaatt ccatctcgcg tcagagaatg gaactgttct
    18481 tcccgaactg gcaggatgcc gccaaaaagc aggcttctgc tgatgccaaa gcggcggctt
    18541 cggctgccag cgctggagtt tcggccacgg cgtatcttaa aactgagcct cttccaaaaa
    18601 cggcgaaacc caccgccgag aaaaaaccag cagccaaggc tgctgccaag aaacctgccg
    18661 caggcaaatc ttccacggca aaagccaagg ccccggccgc gaagaaatct gcaagcacaa
    18721 agaccaaaaa gaagtaacgc atggccatcc gcgcagtatt tttcgatttc ggaggcgtga
    18781 tcatgcgcac cgagtaccag tcaccgcgcc aacatttggc ggagcggttc ggcatggact
    18841 acgatgacat tgacaaggtc gttttcgcga gcgaatctgc gcggcgtgcc tcggtaggtg
    18901 ctattactga agatgcccat tgggccgagg tgctgaaacg cttgaaacac cccgcttcgg
    18961 agatgaaatc tttcaaagat gaattcttcg gcggtgatgt gattgaccgc aatctggtgg
    19021 aattcatccg ctctttgcgg gggcaggtcc atacagggct gatctccaat gcatggagcg
    19081 gattgcgtga gttcatcacc agcgaaaagg tcatcactgc cttcgattcg gtcacgatct
    19141 cggcggaggt tggtgtggtc aagcctgaag ggcggatcta tcaagttgcg ttggatcagg
    19201 ccaaagttaa ggcagaggaa gcggtcttcg tcgacgatat gaatatcaac ctcgaaggct
    19261 gtgaagacct gggcatgacc ggcatcctgt tcaaaaatcc acaggaagcc attgaggaac
    19321 tgaaaaggct gctggcatag tttttgcaac acaggttcca cattttcgat aaagttttga
    19381 aacttcagcg tctaacccac gctgttgtct tttaaggagt atgcgatgaa aatcatcatc
    19441 ttttcaaaca ctgaccggca aacggagttc gctctccgtg cctgatgact ggcttttatt
    19501 aaaaaaggaa cagcatgttt atcaatttaa agctggatgc aaaaaaagta tatctattca
    19561 ttgaatttac ggcatcggca cttttcgcga tgatgttcgt aacaatgtcg ttgtatgaag
    19621 ccacggtggc agggctgacc ccgttgcagc ttgtattggt gggaactgcg ttggaggtct
    19681 cagcttttgt tttcgaaatt ccaacaggcg tggtcgcaga tgtgtattcg cgccgacttt
    19741 ccatcatcat cggttatttt ttgatgggaa tcggtttttt ggtggaaggg ctcttccctg
    19801 cctttctgcc gatcctgctg gcacaggtga tttgggggtt gggatatacc tttaccagcg
    19861 gagctacaca agcctggatc tcggatgaaa ttggcgaaga agaggcaaac aagctggtgc
    19921 tgcgcgccaa ccgcgttggg ctgtacgcct ccctgcttgg aatgggaatg gcggttttga
    19981 tcggctcaca atcggtgggg ctgcccattt tatttggcgg ggcaggtgtg gttttgatcg
    20041 ggcttgtctt ggtggtcatt atgcctgaga ccggtttcca ccccactccc agagaagacc
    20101 gcaacaactg gcagcacatg gcgcatactt tcaaggaagg catcaaggct gtccgtgcgc
    20161 gcccgcgcct gatgaccatt ctgggaattg ggctgttata tggtttgtat agcgaaggct
    20221 ttgaccgttt gtgggtaaaa cacattcttg ataccttcga attgcctgtc ctcttcgggc
    20281 aaaccgatgt ggccttcttt ggtctgctgc gcgcagggag catggtcctt tccattttcg
    20341 caacccgatt cgtggagaag cgaatcgata cgggcagtcc gctggccatt gggcgtgcca
    20401 ctctgggaat taccttcggc atctctgccg ggctgatcgg ctttgcgctt tcgcccattt
    20461 ttgccgtgac gatcatcgtc tactggttta tcagtgtcct gcgaaatgtg gccggtccgc
    20521 tttacaacac gtgggtgaat caaaaactcg accccaatac acgcgccacc gtactttcca
    20581 tgtcttcaca ggtggatgcc attgggcaaa tggggggcgg accgctggca gcggcagtgg
    20641 ctggaactgt ttctgtggtt gccgcgatca ttacatcggg actgctgcta ctgccagccc
    20701 tgccattgat cggacgggcc aattcacaat ccattgcaga gaaggttgag caaaccgttt
    20761 cataaacgcg ggggttagtt aattcataat aaaagggaag ctggattctt ttcgatccgg
    20821 cttcccgatt gaatggatta taattttcag gcaatcacca attcaaatcg gaggtgtcta
    20881 gtggatctct cgaagcacgc tttctttgag ggaagaatcg tcccacttgc ggacgcaaaa
    20941 atcaacattg ccacacatgg ctttttatat ggaaccgccg tgttcagcgg tatgcgggca
    21001 tattggaacg aagagaagaa acgtctcttc gtttttcgtc catttgacca ttatcagcgc
    21061 cttttaaact cagcccgcat gatggccatg gatattccgt acgacgaaga gagtctgata
    21121 caacttaccc ttgacctttt gcgcacagat aactggcaac aggatgtgta cctgcgtccc
    21181 accatctaca aggcagatat gggcattggg gtacgcctgc acaacttaaa agatgagttc
    21241 tccatgttcg tgatggcttt cgaaaaatat gtccccaatg atgcgaatgc tcacatcact
    21301 ttctcatcct ggcggcgtat tgacgataac tccataccag cgcgcgggaa gatagcggga
    21361 gcatatgcca actctgcttt aatcaagacc gacgccaatc gcgcaggctt cgatgaagct
    21421 ctggtgctcg ataacaacgg ccatgtttct gaaggctccg ccatgaacat cttcattgtc
    21481 cgtgatgggg ttctgatcac tccacccgtc accgataaca tcctcgaagg gatcacccgc
    21541 cgttcggtta ttgaactggc acgcaacgaa ctagggctgc aagtacaaga acgttccatt
    21601 gaccgcactg aagtgttcat tgctgaagaa gtgttcatga caggcaccgc cgcccagatc
    21661 gtagctgtga caaagatcga ccaccgcccg gttggcatcg gaaaaatggg acccatcaca
    21721 gaaaaactgc gcggtttgtt cgatgaggtt gtccgcgcaa aactccccaa atacaggcag
    21781 tggaacctgg aagtttagag aaaaggtaca aagtttctca tgaaaaaagg ccagccttgt
    21841 gaggctggcc tttttgattt attcacataa aaacggtcac ttcttcctgc gtgcttcctt
    21901 ctttttcccc tctccttttt tcagttcctc aagttccaat cggttctttt ccaattccaa
    21961 ttcaagtttt ttacgttcca cttcagccag tgaagcctcc cgttgttcct tgcgccatgt
    22021 gatgaccgtg gtgataataa aacccaccag cgaagtgacg gaagtgacca cagaaccaat
    22081 gaccgcggta atgcccattc cattggcatc ctgcgagggc ggggtcgaag atgggtcgtc
    22141 accggtggtc gccgatgggg caaattcact gaccgcttcc tttaccaagg aagtgctgac
    22201 cgccattacc acaaacgcaa tggtggaaag caggaacaaa gccagaaaaa tgttgcgaag
    22261 ggcggcccgc ccggtgaata cctgaaacac ggaagcactc ccgaaaaatt acggattctt
    22321 cttgaagcgt accaacacca gatttttcga acattccttg aacgtatcta tctcgatcgg
    22381 gtcggaaagg ggcaggccag cttgatcgag gatctgcaaa gtcaggttat cacgggatgt
    22441 gatcggggtg gtacctaaaa agaactcata tcctgatttt ccataagctg gcgcaatact
    22501 gctgacagtc aactcgttct tggtgctgcc gttgtaactc cctacaagac ggacggtgat
    22561 cctgagaata tcggcattgt tcgcatccac aattgtgccg ccaatgccct gccatttaca
    22621 gtcggcttca gggtggatga tcgtactatc aatgtaggtt actgtagcgg aaaattgagc
    22681 cttgatcggc gttggagtgg gagtatcaga aggagtgata agagagaaaa gggtcggcga
    22741 agggatcaat gtatgcgtgg ggagcagagt cggtgtttcg gtcacaattg agaaaggagt
    22801 ggcggtccag gtggcatcca gttgcagcgg tgtgatcgtc gctgtgggcg gcgcatttgg
    22861 gtcaaccatg ctggccttca aaggattgat ggccgactgg gggaaaataa agatcgcgac
    22921 aaaatagacg cccaaacaaa gcgtcagcaa aagcgtcgca attgtcagca tatcccagac
    22981 ctcaagtttt gcgctctgct ttgggttgga ggtcgcttca tagttataat cgtatttgct
    23041 cattacttag ctccaatgac aacttatctc actgcaacaa cgggttgcat gcaaactagg
    23101 gaattaaagt aacatcactc tttgcgcgct gatcagcaag ccagttgatg actgcacgtt
    23161 cctgaacgac cagcaaggca tcaggagtca gcgggtggtc gccgcgttca acaactttga
    23221 aaatatgaaa tcccgcatct gttgcgatca catctgaata agcgcctggc tgaagggcaa
    23281 acaacgcctc atctgccgcc gggtcgagca ggtaacctcg tggcacccaa cccaattcac
    23341 caaacgtgat cggatcgaaa aatgcagcct tctcatcgaa atctgcaccg gctttaattt
    23401 catccaaagc tgcgcgtgca tccgcctcat tataggtcaa aatctggcga acgtggattt
    23461 gttccacaac cgttggcaca tccgcaatga ttttgtcacg catccatgct gcttcaatgg
    23521 aacgggtcaa cgcaaagcgg aaggaagcat cgtcataccc gtgggtggat tgccactgac
    23581 ttaaagcgtc ggcactgcct aattgagctg ccaacgcatc aatccttgac tgtaagtctg
    23641 cctctgttaa agtgaaattc gctgctctgg cggcttgtgc cagcaaaacc tgcgagatca
    23701 tatcttcgag cacagccttg gccgcatctt cctctgtgaa ggagagtccc aaggcagttt
    23761 gggcagactt gtatcgttcc aattccgcct gaaattcagc ggtggtaatg tactcaccat
    23821 tgaccgtcgc cacggaaggc ggtggagtgg cagtgggagg caaaggtgtg gaagtaggca
    23881 attcgggagt gggcgtgttc gaggtcccga acgaagcgca ggcacttaat cccaaaacaa
    23941 aagccagagt caattgagca aaaaaacggg gtttggaaaa cattcgtgga attatatatt
    24001 aaaaaagtag ggacacggcg ttgccgtgtc cctacaaatt tgtttatggg caggtttagt
    24061 aatccatgcc gcccatgccg ccaggaggca tggcaggagc cttgtccttc tcgggcatat
    24121 cggtgatgag cacatcggtg gtgaggatca tcgcggcgat ggaagcagcg ttttcgagag
    24181 cgccacgaac caccttcacg gggtcgatca caccagcctt gatcatgtcg gtgtattcgc
    24241 cggtgaggac gttgtaaccg atattcggat tcttcttctc ggcagcggtg cggcggacgg
    24301 tgtcaatgat gacggagcca tcctgaccag cgttggaaga aagcttgcgg atcggggctt
    24361 cgagcgcctt cttgacgata ttgatgccga ccttgatttc ttcgttctca tcttcagcgt
    24421 gagccttggc gagtttgtcg agtttgccag cggcattgat gagggagatt tcaccaccag
    24481 ggacgatgcc ttcttcaacg gctgcgcggg cagcggagag agcatcttca acgcggtgct
    24541 tcttttcctt catttcagtt tcggtcgcgg cacctacgcg gatgatggca acaccaccgc
    24601 tgagcttggc aaggcgttcc tgaagttttt ccttgtcata gtcgctggtg ctcttgtcca
    24661 tctcaatgcg gatttctttg atgcgggctt cgatatcacc cttcttgcct ttgccgccaa
    24721 cgatggtggt gttttccttg tcggaaacga ccttctcagc gcggccgagg tcctgcacgg
    24781 tggtggattc gagcttgcgg ccggtctctt cagagatgac ctgtccaccg gtgagggagg
    24841 cgatatcctg aagcatggcc ttgcggcgat caccaaagcc aggagccttg atggcgagca
    24901 cgttgagcat gccgcggatc ttgttgagga tgagggtggc aagagcttcg ccgtccacat
    24961 cttcagcgat gatgac
//
LOCUS       DAOQLC010000063        24916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_665_length_24916_cov_24.222316, whole genome shotgun sequence.
ACCESSION   DAOQLC010000063 DAOQLC010000000
VERSION     DAOQLC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_665_length_24916_cov_24.222316"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..530
                     /locus_tag="PL000_12205"
     CDS             54..530
                     /locus_tag="PL000_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HNC89709.1"
                     /translation="MRYVEWQAGVPDEIKGDSLWKLEVYRLGLFAADIAWQDALVLNK
                     NPLTRGLADQLYRAVCSISANIAEGYSRSTGKDRARFLEYSLGSARESRDWYFKSRHT
                     LKEEVVKHRIDLLTQIIKMLSILTPNQRQKGIREEQAEYIISQPSEFNIDLETPLP"
     gene            566..1051
                     /locus_tag="PL000_12210"
     CDS             566..1051
                     /locus_tag="PL000_12210"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HNC89710.1"
                     /translation="MTFSNSIDTPHHFLAQLAGNWRGTSKLWMEPGKLADESQIVGTI
                     QLLLEGRFALFLYQSSIEGEAQHGMFTFGYNTSLEQFETSWVDSFHTNTGIMFCTGRE
                     LGGGFSVTGSYPDPSGGPDWNWRTEVELNGDQLTITAYNITPEGEEAKATEMALTRLK
                     K"
     gene            1174..>2063
                     /locus_tag="PL000_12215"
     CDS             1174..>2063
                     /locus_tag="PL000_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC89711.1"
                     /translation="MKILIIGGTRFLGRHLVESAFARGHEVTLFNRGKSNPELFKQVQ
                     TIRGDREKDLDQISGKYDAVIDTCGYFPRIVRMSAEALKGKARSYVFISSISVYAGFS
                     KIGINESDSVGKIEDETIEEITGESYGPLKALCENAVQDVFGEQALIVRPGLIVGPHD
                     PTDRFTYWPVRVARGGDVLTPEKPDVPIQIIDVRDLSDFVIELLQQNEFGVFNATGPA
                     HELTLGAMLNACKQVSGSGANFKWASVEFLNQNQVAPWSDMPVWVPNVPEDAGFSRVD
                     ISKAMRSGLKFMPLEKTIHDT"
     assembly_gap    2064..2163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2164..3639
                     /locus_tag="PL000_12220"
     CDS             <2164..3639
                     /locus_tag="PL000_12220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC89712.1"
                     /translation="RGEIAIRIMRACRELGIKTVAVYSEADKDAQHVQFADESVLIGS
                     AAPKESYLNADALLRAALDSKAEAIHPGYGFLSENASFALKVESAGLTFIGPSADSIR
                     AMGDKAESKIAMKKAGVPTVPGFEGLTSEEEFKKAAQEIGYPVLIKASAGGGGKGMRV
                     VNFENELSEAIESARREALNSFGDERLLIEKYLADAHHIEFQVFGDKHGNLVHLFERE
                     CSVQRRHQKIIEETPSPLLTPEIRAKMGEAAVAAAKAVNYYNAGTIEFIFDPQLATLN
                     LQPFYFLEMNTRLQVEHPITELTAGIDLVQWQIRIAAGEKFPFTQGDFHQHGHAIECR
                     VYAEDPSNGFLPSTGKLLQLIEPRGPGIRVDSGFKTGSEVTHFYDPLLAKLIVHAENR
                     NAAIQRMQTALREFVVHGVVTNMDFMQAVLQHDDFAQGKVSTRWVERNFGEWHPVEAE
                     KEALIAAALADVLFVGGKTQSAVSNETDPFNPWKQTGNYRN"
     gene            3642..4160
                     /locus_tag="PL000_12225"
     CDS             3642..4160
                     /locus_tag="PL000_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HNC89713.1"
                     /translation="MKTTFDTQTLEITPSGDHYLAVMDGRKILVEVIRVDAAQGRMDL
                     RFTELSESDSIRVSVSKSVYVSSDMAKRWVTVDGHTTMLTKTSGAKQGVRHDHAGGLI
                     APMPGQVRSVAVGVGDVVKKGQTLLTMEAMKMEIRIQALKDGVVSALPVQVGQTVERE
                     QILIEIVDRVEH"
     gene            4203..4700
                     /locus_tag="PL000_12230"
     CDS             4203..4700
                     /locus_tag="PL000_12230"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNC89714.1"
                     /translation="MAGKYFEDLDVGMHFKHSAGRTVTEMDNVLFSSLTMNTQPLHVN
                     EDFAANTEFGQRIVNGIFTLGLVVGLTVAELSEGTIIANLGYDKVVHPNPTFHNDTIY
                     AETEVLEKRESKSRSNAGIVKFRHTGKKSDGTIVVQFERTALFLKKGQRLDFLPRSES
                     QSYDL"
     gene            4754..5620
                     /locus_tag="PL000_12235"
     CDS             4754..5620
                     /locus_tag="PL000_12235"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HNC89715.1"
                     /translation="MHSRRALLYMPGDNWKMITKSITLGVDSICMDMEDGTAINKKAE
                     ARETIAKALRELDFGSSEKLARINSVGSGWEQDDIDAVLPFHPDGIVIPKVESFEQVE
                     WASRIIETAELKYGWPVNSVRVLIGVETAKGILNLKEIAAHPRLDAIIFGGEDFAASI
                     GAVRTKDAVELLYARQAVIVACSAFDLQPIDIVTIDYKDIEALRVESEFGARLGFVGK
                     QIIHPAQVEPVQRAFTPSDEAIAYAKRIVETFEASQKEGKGAYSLDGKMIDMPLLRNA
                     KKVLDRALDRGL"
     gene            5679..6215
                     /locus_tag="PL000_12240"
     CDS             5679..6215
                     /locus_tag="PL000_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC89716.1"
                     /translation="MKWSTSPHRPPHLYVDGAWYFITSSTVGSVKILSTDDHLNLWVE
                     TFKELILQFKIKLIAWVILPNHYHILFMPEKADELGSFMKRLNGNTSRKLNLFDDQAG
                     RSVWYSYWDTCIRNERDFWTRFNYIHYNPVKHGYVEYPGEWRFSSYSFYLRNDESPWL
                     AGYEKDFPIYDLFDDDRF"
     gene            6288..7133
                     /locus_tag="PL000_12245"
     CDS             6288..7133
                     /locus_tag="PL000_12245"
                     /inference="COORDINATES: protein motif:HMM:NF018506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HNC89717.1"
                     /translation="MLSIFLGLLAALGWGAGDFTGGIASRKTGAYRTVFYGELIGVFA
                     LFLVIAIISEPIPDLRSWLFAMLAGALGTLGLILLYHAMAIGVMSIAAPVSALLAAAL
                     PVVVGIFREGLPAWTTFIGFGFALFAVWMISQGEGGVTDIFSHLADLKLPLIAGIGFG
                     SYFVFMHEATSTGSTIWPMVASRSGGMILITIYMLVVRVEWKVEDRSAWPIIVLNGIL
                     DISGNVFFILAGQMGRLDVAAVLSSLFPGTTVMLAWIFLKERLSPTQWIGIASALTAI
                     VLMTI"
     gene            7142..7798
                     /locus_tag="PL000_12250"
     CDS             7142..7798
                     /locus_tag="PL000_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNC89718.1"
                     /translation="MNPYLLFAFIFALLEALALWKGWIRLEYVAKPAVMIVLLLWLWT
                     SVGISGALLWFGVGILFSLLGDVLLMISLDRMFLLGLVAFLLAHIAYVIGFNIPLPVF
                     SAWSLVFAFMVGLGGTRIIRRILEAIGNNRMRKPVIVYSLVISLMLLSAMMKLTDVTW
                     DAGAAALVSLGAFLFYLSDIVLAWNKFVAPIQNGRIYNIGLYHLGQIALIAGVIAQFG
                     "
     gene            7850..8287
                     /locus_tag="PL000_12255"
     CDS             7850..8287
                     /locus_tag="PL000_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89719.1"
                     /translation="MQSILVIIIIGLVGGMAVGLQAPMSSMISQRLGVLESIFIIHLG
                     GALVVLLPLLYFGGGKLGHWRTVPWYALCAGAFGLVVIFSMSYMIPRIGIATALIILL
                     AGQILIGSILDHFGLLGAAVRPLDFTRVLGLGVVLAGVWLAMK"
     gene            8370..9134
                     /locus_tag="PL000_12260"
     CDS             8370..9134
                     /locus_tag="PL000_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89720.1"
                     /translation="MYNYPLKFEFPMISLGRQMDVKNGDGGFIMRVSDPYISFKDEMT
                     VINGKGAAVMKAKGDSSFRFLFQFASTSTSWNIVDGSDRTIAWVTNYWARMEEFKDVY
                     VDTPSGVGADGRADIGGVLGNIATNVLNNRVASTLPSRLVYKITDSEYGNVLGWVVPS
                     RGTGWFDFLPYSTRIQILNLPFAARAYAPSYEFKLGNLYGPTVLKLQKQRDLLVDKYT
                     LEKIGDLSEANERWALPSIALAAMFERQRVKEMADW"
     gene            complement(9242..11122)
                     /locus_tag="PL000_12265"
     CDS             complement(9242..11122)
                     /locus_tag="PL000_12265"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89721.1"
                     /translation="MAGSRKLQYADRVALQQADQAIRKDVLRALVEIITNSNDSYSRL
                     EHAGLKVSGEIIIDIQRKHKNSIIRVTDRAEGMTDTRMDKVVGTYGEATSGLKEDKNV
                     RGMWGRGLKDSIFGLGYGYVHSFKGAYFYSCALLLKNGIPTFELQPPVRATVPLREKY
                     GIPEGNGTILEIIVSRDDVKVPQFDNFRNYLQRHFELRPIMSNPKRKIILRDVPSAEK
                     IRHEHILSYKSPRGEKVLSEKIKIEGYPAAAKLEVFRSAIQLSTRGEEGDYADGGLLV
                     ISRATVISLTMLKFENDPYAGYFYGSIQCDHLHDLLKNDEPVLTATRDGINWSHPFAK
                     ALKLAVENKLEPLIEAERKHAVQDEQTHLDKQFRQKIDHALHELNTIALNELSDRRDD
                     GDQKRIDLPPSGIGFFPERVHVQTGQTISLTLFVKLSEKVRPGATVTVISNSPEVIVL
                     NQQAVLQPHKTDDSVGRAHIRVEGRQVGGEGLVTAYIGKQRAQALVQVRSKKETLTIA
                     PSRSSNALFTDIHFDDRTDPRQRVYFDRVNSSIVIATAAPSVKLYLDKQNRLDTTVQG
                     QVLLAELITEAVCREIARSGVERGRFLAPDGAEADAINNHFIRLQNQYSHLIHKYIVT
                     LE"
     gene            complement(11149..>11595)
                     /locus_tag="PL000_12270"
     CDS             complement(11149..>11595)
                     /locus_tag="PL000_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89722.1"
                     /translation="KKKEVAVVTPVVLKPAKKKEEKAVVPVVSEPVKKKEEKAVVPVV
                     VSQAVSREEKARAAKFVRAMEVSDAYRLYKKNGKESKISEFDFRSLLLCTMESSSETL
                     ARNVELYKGYASIHNRQDLLTFLGYCETSFETLLKPGPVKKAAKKK"
     assembly_gap    11596..11695
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11696..12239)
                     /locus_tag="PL000_12275"
     CDS             complement(<11696..12239)
                     /locus_tag="PL000_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89723.1"
                     /translation="MTAKTSGARKLKRRSLPKFDPSVYSAISSVNLVAYAIHFLLERG
                     IEIHMEDVVFGCFLLFPHKFALKKYPRWPDSAVVGRRVGDCRSKGYIAANTEFGFKLT
                     VKGTLAAERAAKALGIFKVQKPGLPQKPAEKVIAQQAVQLTLLETKAVVAPVVVSEPV
                     KKKEAAVVAPVATSKPVKKKE"
     gene            12368..14098
                     /gene="polX"
                     /locus_tag="PL000_12280"
     CDS             12368..14098
                     /gene="polX"
                     /locus_tag="PL000_12280"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HNC89724.1"
                     /translation="MMNNRQLADTFTLIANLCEIKGEVIYVILAYRKASENLMTLGRE
                     ASEYWKEGKLREIPGVGKAIAEKIDELLTTGKLQFLENLKEEVPASLADWLQVPGLGP
                     KKIALIWKTLNITALSELETAAKNGQLRDLPGMGAKSESAILEGIASLARRSGRIPLG
                     RAYPLAQEIIRTLKRVDGVVAAESAGSLRRMRSTVGDLDILVASTNSAAVMEAFTNLP
                     GVSRVLGKGETKSSIEFTDGVRAQVWVHAPEKFGTALQYATGSKDHNVQLRQIALAKG
                     LSLSEHSLTKTNGKGEIFCATEEEVYERLGLPWIPPELREDHGEVVAAKAGSLPKLIQ
                     VKDIKADLQMHSNWSDGKLTMLEMAQAAIAHGLKVIAFTDHSASLGVTGGLKMEDHKK
                     QAAEIKKVQKQLGDKILILHASEVEIKADGTLDYPDEFLASLDLVLASMHTSLRQPRE
                     KVTERMIAAIRNPHVDIIGHPTGRLIPDREGADLDMEAVLNAAAETGVALEINAHPSR
                     LDLDDMYARRAKELGIPISINTDSHSAEDFDNLFYGVATARRAWLTKEDVINTWSTKK
                     LLNWLEKRGK"
     gene            14381..>15354
                     /locus_tag="PL000_12285"
     CDS             14381..>15354
                     /locus_tag="PL000_12285"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNC89725.1"
                     /translation="MAESLIPANEVRFFSGSSNPQLAGKIALHLGVPLEDTHISRFSN
                     DNLYIQLGASVRYRRVYIVQSLSKPVNDHLMELMMMIDIARSAAASEVHAIIPYFSFG
                     RSDKKDAPRISITARLVADLLKTAGATHVMSIMFHSPQVHGFFGIPTDPLSSSKVFKE
                     YLSKSDLSGSILVAPDMGQAKTAARFARTLNLPIAAGNKERVSDTKVIINGLVGDQVR
                     GHERALIYDDEIATGGSILELSRVLISEGLKDIRVICTHGVFSNNGLERLAEVPEITE
                     IITTDTVHIEPERVHPKLTVLSVAPVFANAIRHNINRESLSDLFVYGE"
     assembly_gap    15355..15454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15556..16050
                     /locus_tag="PL000_12290"
     CDS             15556..16050
                     /locus_tag="PL000_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNC89726.1"
                     /translation="MINKTMQDAMNDQINKELFSSYLYLSMAAYFEDKNLPGFAHWMR
                     IQEAEEREHAMKFYDFILERGGRVMLKAIEAPKTDWKSALEVAEEVAAHEAKVTASIH
                     ALYELALKEKDYPAQVMLQWFISEQVEEEKNAAEIVANLKLIEERGTAVLMLDHRLGK
                     RGGE"
     gene            16109..16549
                     /locus_tag="PL000_12295"
     CDS             16109..16549
                     /locus_tag="PL000_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89727.1"
                     /translation="MQITIRRATHADKGEWFQMRKGIWPDVPDHYMSFDMDDLLASEN
                     YLVLFACDGDTPIGLTEARLRDHAEGCSTSPVGYIESWYVREDYRGRGVTGIMNQAAE
                     EWARSKGCTEMASDTWLDNEASIRAHLKLGYFEVERLVHFVKKL"
     gene            16787..18697
                     /locus_tag="PL000_12300"
     CDS             16787..18697
                     /locus_tag="PL000_12300"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89728.1"
                     /translation="MSSIRLRVVIPYLLLTLIVSGIGVYVVTNLVSNTLSERLANQLV
                     QSGRVVLDGFVKQEQSQIEDARFIAFTSGVADALVSGTADGLLVEQATTQMTGRDIDT
                     LVFISSQGNEILHLWKNDLNVVQRANENSGMAGSPLVTPFLASRNTRDNPRHFLGTNS
                     LNDRTYFYASIPASLNGEFAGVVVIGIDIEKLLPNLKSSALADVVVYGADGSVIATTL
                     DVSDPLSLEALSITNDNYQRISALTDLVTGSNISIDRRSYSVARGSLQLGNDKLGVFA
                     VALPLDFVNQAVSENQTTYTLLFIGVFFFVIVIGLWVSRSIVNPLYQLVETSQAISAG
                     DLEKRTGIKSRDEIGILASAFDEMTANLQERNAQLERANQILKNIDKTKSNFIQISAH
                     ELRTPLTLIMGYSQMLEQDARNDPELKSLAQGILDGSERMTSIVESMLDVSRIDSNSL
                     FLRKTGLRIDLIVRKVYKNFEKALVERDLQFDMNNLRDLPLIPADPDMLQKVFYHVIM
                     NAIKFTPDGGSITISGRYVPANEPSVEISIRDTGIGINPEMHSLIFEKFNQTGDVLLH
                     SSGKTKFKGGGPGLGLAIARGIVEAHGGRIWVESPGYNEETNPGSTFHISLPAPEDQQ
                     EEVRREPAISKQ"
     gene            complement(18902..>19321)
                     /locus_tag="PL000_12305"
     CDS             complement(18902..>19321)
                     /locus_tag="PL000_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89729.1"
                     /translation="VAVFVAVGMTVNVAVAEAVGVDVGVTVAVEVEVAVLVGVAVLVA
                     VNKDVAVRVAVMVGVRDTEVEVRIGVRVAVRVDVTATTVERLVLVAVGGEVRVAVGAE
                     RWVEVAVAGGRWVKVAEPFGLIGPEEEPEAVPVPGVR"
     assembly_gap    19322..19421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19671..20165
                     /locus_tag="PL000_12310"
     CDS             19671..20165
                     /locus_tag="PL000_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNC89730.1"
                     /translation="MRKSTLFISAMLTVFLMATLFGVISAYQNIMATNQAVVVTPAEL
                     SVQEPAATAVPTIAAPMVISPEQATAIAVELLGDTNVYSVEVVDYEGVAAYLVTFSAG
                     DLVYVSSTGEVLAVTKLEPVVMVVPAGNRNDSGEKPNRDQQQQEDDGDDDDHGEDEHE
                     DGDD"
     gene            20180..20509
                     /locus_tag="PL000_12315"
     CDS             20180..20509
                     /locus_tag="PL000_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89731.1"
                     /translation="MNPKPAPRKWTDIQLAIAAVSMTSVLAFWNMFAGPDRSKSEEKA
                     AEAQQASQAAPTSAVMSVSPAPTMPPAGYTILFGGTAPQPQVIVMQSGQGGNQGGGDP
                     VTVTTSS"
     gene            20539..21429
                     /locus_tag="PL000_12320"
     CDS             20539..21429
                     /locus_tag="PL000_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC89732.1"
                     /translation="MGSDILVILEQDSEAKPAILKDIPAWFENWEQTLSRFRPTSELS
                     HLNRTFDQPIKVSDAFWDVFQTALWADEISGGLVTPTVLDSMVEAGYDRTFDKLPRSQ
                     FDMIASPAEADHPLSTVISDCDAKTINLPKGTGLDFGGVAKGWAAHQAVERLQEFGPC
                     LVNAGGDIAVSGPRMDGSPWPVGVSNPFNVGANINVLQVKQGGIATSGKDRRNWNRNG
                     IFYHHIINPLTGLPAETDLLRVTVVAPTTMQAEAAAKTAFILGQEKGLHWIEAQPEFA
                     GLLVLETGDVIHSRRMQKYL"
     gene            21444..22178
                     /locus_tag="PL000_12325"
     CDS             21444..22178
                     /locus_tag="PL000_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89733.1"
                     /translation="MNAQPQSEFNESSVNIQSFLLFLFATVIGLLVAVLLLPIFLPNM
                     AFSLSGDSPKAYWYLSRATAFVSLSLLWLSMALGLGITNKMARLWPGMPATFAIHEYV
                     SLLGLAFAIFHALVILGDHYINFTLLQLFIPFSTVEYRPFWVGVGQIGFYIWAIVALS
                     FYIRPVIGQKFWRFLHYASFGMYGMGILHGIFSGTDTSLAWAQNYYWYSAGSLIFLFF
                     VRIVGSLMDVLFPAKKQTPAARPTQG"
     gene            complement(22259..24151)
                     /gene="dnaK"
                     /locus_tag="PL000_12330"
     CDS             complement(22259..24151)
                     /gene="dnaK"
                     /locus_tag="PL000_12330"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC89734.1"
                     /translation="MGKIIGIDLGTTNSVVSVMEGGSPTVITTAEGGRLCPSVVAFNK
                     NGERMVGQTAKRQAVVNADNTIYSIKRFIGRHFEETSTERSMVPYQVIQGPTGDVRVK
                     IPVTGREYSPQEISAMVLGKLKADAEAYLGEPVTQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKKNETILVFDLGGGTFDVSILEVGEGVIEVKATNG
                     DTHLGGDDWDQKITNWAADEFKKDQGIDLRQDRQALQRLREAAEKAKIELSTMMETEI
                     NLPYITADASGPKHMQLKLSRAKFEQMTEDLLQRCRKPFEAALKDAGMDAGKLDEVVL
                     VGGSSRMPMVQDLVRKLTNGKDPNKGVNPDEVVAIGAAIQGGVLGGEVKDILLLDVTP
                     LSLGVETMGSVMTVMIERNTTIPVRKTEVYSTAADNQTAVDIHVLQGERPMANDNMSL
                     GRFRLDGIPPAPRGIPQVEVTFDIDANGILHVTAKDKASGKEQKVTITASTNLNKNDI
                     DRMVQEARQNEAADKKRREVIEVKNTADNLSYQIEKSLRDLGDKVPAAERDAIEAKVN
                     QLKQAAQGDDVDQIKKLTEELQNTFHALSQQMYAQGQPQPEGGPAPSGPSGHANGDVI
                     DGEVKE"
     gene            complement(24293..>24916)
                     /locus_tag="PL000_12335"
     CDS             complement(24293..>24916)
                     /locus_tag="PL000_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89735.1"
                     /translation="PATATHFATFTPTPTLTATPVPMYFTEEYNTDMTAWSSFQTGGE
                     LSPSLEIVDDRLRLDLFSPNTWYYAIHNSHEYQDVSITAKFSGTPAGSAGVICRYSED
                     GWFEYNINSDGTYSVLLGQWLADGIASYTPIVNASVEYITPGMDYEIGLTCQGTFLLL
                     HANGKMFRKLDVARYPLVNGKVGITAASFEEAPAIVMFDWFKISQPE"
BASE COUNT         5623 a         6070 c         6487 g         6336 t
ORIGIN      
        1 gggtttgggg tgtttaggat gtagaaagtg gaaagtggtg agtggaggat ggcatgaggt
       61 atgttgaatg gcaagcgggt gttcctgatg aaatcaaagg tgattcgctt tggaaattag
      121 aagtctatcg cttaggttta tttgccgccg acattgcatg gcaggatgcg cttgttttga
      181 ataaaaatcc attaacccgt ggacttgccg accaactcta tcgagcggtg tgctctatat
      241 ctgcaaacat agctgaaggg tactcgcgtt ctacaggaaa agaccgtgct cggtttctcg
      301 aatactcact tggttctgca agagaatctc gtgattggta tttcaaatca cgccatacgc
      361 tgaaagaaga agttgtcaaa catcgaattg acttgttgac tcaaataatc aaaatgctaa
      421 gcattcttac acccaatcaa cgccaaaaag gaatccgcga agaacaagct gaatacatca
      481 tttcacaacc atcagaattc aatattgatt tagagactcc actcccataa tccactcacc
      541 actttccact cactagaggt actccatgac cttctcaaac tcaatcgaca cccctcatca
      601 cttcctcgcc cagcttgctg gtaactggcg cggcacatcc aaactttgga tggagccagg
      661 caaacttgca gatgaatctc agatcgttgg aaccatccaa ctcctgctgg aaggacgctt
      721 tgcccttttc ctgtatcaat cgtccattga aggtgaagca cagcatggca tgtttacctt
      781 tggctataac accagcctcg aacaattcga aacctcatgg gtggattcgt tccacaccaa
      841 cacgggcatc atgttttgca caggcaggga attgggcggc ggtttctctg tcactggcag
      901 ttatcccgac cccagcggag gcccggattg gaactggcgc accgaagtgg aattgaacgg
      961 cgatcaactg accatcaccg cctacaacat cacccctgaa ggggaagaag ccaaggccac
     1021 cgaaatggcg ctgacaagat tgaagaaata aaagtgtcat gtcgttgcga ggagcgctct
     1081 tgttttttgc gacgaagcaa cctccaatta cgaacgagat tgcttcgtcg ggacgaacac
     1141 cctcctcgca acgacatatc aaatggagac aaaatgaaaa tcctcatcat tggcggcaca
     1201 cgttttttag gtaggcatct cgttgagtct gcatttgctc gcggacatga agtgaccttg
     1261 ttcaaccgcg gcaaatccaa ccctgaactg tttaagcaag tccaaaccat caggggtgat
     1321 cgtgaaaaag atctggatca gatttcaggg aagtatgatg cggtcattga tacctgcgga
     1381 tattttccgc gcatcgtccg catgtccgcc gaagcgttga agggcaaggc ccggtcttat
     1441 gtttttatct caagcatttc ggtgtacgca ggtttcagca aaattggaat caacgaaagc
     1501 gactccgttg gaaagattga agatgaaacc atcgaagaga tcacgggcga gtcgtatggc
     1561 ccgttgaagg cattgtgtga aaacgccgtg caggatgtct ttggcgaaca ggcattgatc
     1621 gtccgccctg gactcattgt tggcccgcat gaccccactg accgcttcac ctattggcct
     1681 gtgcgcgtgg ctcgcggcgg cgatgtgctc acgcccgaaa agcccgatgt gccaattcaa
     1741 attattgatg tccgtgacct gtccgatttt gtcattgagc ttcttcaaca aaatgagttt
     1801 ggcgttttca atgcaaccgg cccagcgcat gaactgactt tgggggcaat gttgaacgcc
     1861 tgtaaacagg tcagcggcag cggcgcgaat ttcaaatggg catcggttga gttcctaaat
     1921 cagaaccagg ttgcgccgtg gagcgatatg cctgtttggg ttcccaatgt gcctgaagat
     1981 gcaggcttct cccgtgtgga tatttcaaaa gcgatgcgat ctggcttgaa atttatgccg
     2041 ctcgaaaaaa cgattcatga cacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnncgcggcg agatcgccat ccgcatcatg cgcgcctgcc gcgaactcgg catcaaaacc
     2221 gttgccgtgt attccgaagc ggataaagat gcacagcatg ttcagtttgc cgatgaatcc
     2281 gttttgatcg gcagcgccgc gccgaaagaa tcatacctaa atgcggatgc cttgctccgg
     2341 gccgcactcg actcaaaagc agaggccatt catccgggct atggatttct ctcggagaac
     2401 gcatccttcg ccctgaaagt tgaatcagcg ggactgacat ttatcggccc ttcagcagat
     2461 tcgatccgcg ccatgggcga taaagccgaa tcgaagatcg cgatgaaaaa ggcgggcgtg
     2521 ccaaccgtcc ctggctttga agggctgaca tcagaagaag agttcaaaaa agccgcacag
     2581 gaaattggct accctgtatt gatcaaagcc tctgcgggcg gcggcggcaa aggtatgcgt
     2641 gtggtcaatt ttgaaaatga attgagcgaa gccatcgaat ccgcgcggcg cgaagccttg
     2701 aattccttcg gtgatgaaag gctgctgatc gaaaaatatc tcgcagacgc gcatcacatt
     2761 gagtttcaag tctttggcga taaacatgga aaccttgttc atcttttcga gcgcgaatgt
     2821 tccgtgcagc gcagacatca aaagatcatc gaagagacac cctcgccgtt actcacgcct
     2881 gagatccgcg caaagatggg cgaagccgca gttgcggcgg ccaaagccgt taattactac
     2941 aacgcaggca ccatcgaatt catcttcgac ccccaacttg caactttaaa ccttcaacct
     3001 ttctacttcc tcgaaatgaa cacccgcctg caagtcgagc atcccatcac cgaactcacc
     3061 gcaggcattg accttgtcca gtggcagatt cgtattgcag caggagagaa attccccttc
     3121 acgcaaggtg actttcacca gcacggacat gccatcgagt gccgtgttta cgccgaagac
     3181 ccatccaatg gatttttgcc cagtacagga aaacttttgc aattgatcga accgcgcggt
     3241 ccgggcatcc gcgtggattc gggcttcaaa acgggcagcg aggtgacgca tttctacgat
     3301 ccgctgttgg cgaaattgat cgtccatgct gagaaccgca atgcggccat tcaaagaatg
     3361 caaactgcct tacgcgagtt tgttgttcat ggcgtagtga cgaatatgga tttcatgcag
     3421 gcagtcttgc agcatgatga ctttgcgcag ggcaaagtgt ccacgcgttg ggtggagcgg
     3481 aattttggtg agtggcatcc tgtcgaagct gaaaaagaag cgctgatcgc tgcggcgctt
     3541 gcggatgtgc tttttgtagg cggcaagacc caatccgctg tttcaaatga aaccgatccc
     3601 ttcaacccgt ggaagcagac agggaattat cggaattagc catgaaaaca accttcgaca
     3661 cacaaactct tgagatcact ccgtcgggag atcattacct tgccgtgatg gatggcagaa
     3721 aaatcctggt cgaggtcatt cgtgtggatg cggcgcaggg gcgcatggat ttacgcttca
     3781 ccgaactttc agagtccgat tccatccgtg tatccgtttc aaagtccgtt tacgtctctt
     3841 ccgatatggc gaagcgctgg gtgacggtgg atggtcacac aaccatgctc acgaaaacct
     3901 cgggcgccaa acaaggcgtc agacatgatc atgccggggg attgatcgcc ccgatgccgg
     3961 gtcaagtgcg aagcgtggct gtgggtgtgg gagatgtcgt taagaagggt caaacccttt
     4021 tgacgatgga agccatgaag atggaaatcc gcattcaggc tttgaaagac ggcgtggttt
     4081 cggcgcttcc cgttcaggtt ggtcaaacag tggagaggga gcagattctg attgagatcg
     4141 ttgacagggt ggaacattaa acgttcaacc tttaaacctg caaacgttcc aacggaggtt
     4201 gtatggctgg aaaatatttt gaagacctgg atgtggggat gcatttcaaa cattcggcgg
     4261 ggcgtacggt gactgaaatg gataatgtgc tgttttcttc gctgacgatg aatacgcagc
     4321 cattgcatgt gaacgaggat tttgccgcga acacggaatt tggtcagcgt attgtgaatg
     4381 ggattttcac gcttgggctg gtggtgggcc tgaccgtggc tgaattgagc gagggaacga
     4441 tcattgccaa tttggggtat gacaaagtgg tacatcccaa cccgactttt cataatgata
     4501 cgatctacgc tgagaccgag gtgttggaaa aacgcgagtc gaaatcgcgc tcaaatgcgg
     4561 ggatcgtgaa gttcaggcac acaggtaaaa aatcggatgg tacgattgtg gtccaatttg
     4621 aacgaacggc tttgtttttg aagaaaggtc aacgcctcga ctttttgcca agatccgaaa
     4681 gccaatctta cgacctataa tctggaacgt taaaacgtta gaacgttcca gcgttgaaac
     4741 aggagacctc ctcatgcact cacgacgcgc actactttac atgcctggtg ataactggaa
     4801 gatgatcaca aaatccatca cgctgggcgt ggattcgatt tgcatggata tggaagatgg
     4861 tacggcgatc aataaaaaag ctgaagcgcg cgaaaccatc gcgaaggctt tgcgtgaatt
     4921 ggattttggc tccagcgaaa aactggcgcg gatcaattcg gtcggttcag gttgggagca
     4981 ggatgatatt gatgcggttc tgccgtttca ccccgatggg atcgtgattc caaaggtgga
     5041 atcttttgaa caggtggagt gggcgagccg cattatcgaa actgctgaat tgaaatatgg
     5101 ctggcctgtg aattctgtcc gcgttttgat cggggtggag acggcaaagg gtattttgaa
     5161 tttgaaggag atcgccgcgc atccacgttt ggatgcgatc atctttggag gcgaggattt
     5221 tgccgcctcc atcggcgcgg tccgcacaaa ggatgcagtg gagttgctgt atgcgcggca
     5281 ggctgtgatc gtggcatgtt cggcgtttga ccttcagccc attgatattg tcaccattga
     5341 ttacaaggat atcgaagcct tgcgagttga atctgagttt ggtgcgcggc ttgggttcgt
     5401 gggcaaacag atcatccacc ctgcgcaggt ggagcctgtg caaagggctt ttacgccatc
     5461 ggatgaagcg attgcgtatg caaaacgaat cgttgaaact tttgaggcca gccagaaaga
     5521 gggcaagggt gcgtactcat tggatggcaa gatgatcgat atgccgctgt tgaggaatgc
     5581 gaagaaggtg ttggatcgtg ctttggatcg cggactttag tccgcgtttg atctgtttcg
     5641 cagactaaag tctgcgatcc gagagaggaa aaaatccaat gaaatggtcc acatccccgc
     5701 atcgcccgcc gcacctttat gtggatggcg cctggtattt cattacatcc tccaccgttg
     5761 gttcggtgaa aatcctttcc acagatgacc atttgaactt gtgggttgaa acgttcaagg
     5821 aactcatact ccaatttaag ataaaactga ttgcatgggt gatcctgccg aatcattatc
     5881 atatcctgtt catgccagag aaggctgatg aacttggttc ttttatgaaa cgcctcaacg
     5941 gaaatacttc gcgcaaatta aatctctttg atgatcaggc tggaagatcg gtttggtata
     6001 gttattggga tacatgtatt cgtaatgagc gtgatttttg gacgcggttc aactacattc
     6061 attacaatcc agtcaaacat ggttatgtgg agtatcccgg agaatggcgt ttttccagtt
     6121 attcttttta cctgcgtaac gacgaaagcc catggcttgc gggttacgaa aaagatttcc
     6181 cgatttatga tttgttcgat gatgatagat tttgatgtgg agcgcggact ttagtccgct
     6241 aataataaat gcggactgaa gtccgcaccc ctgaaggaga gtaaacattg ctatctattt
     6301 ttcttggtct tctcgccgcg ctggggtggg gtgcaggcga ctttacgggc ggtattgcct
     6361 cgcgtaagac gggtgcctat cgtacagtgt tttatggcga actcatcggc gtctttgccc
     6421 tgtttcttgt cattgcaata atttctgagc ccatccccga tctgcgttca tggttgtttg
     6481 ccatgttggc gggtgcgctt ggtacgttgg gtctgatcct gctgtaccat gcaatggcca
     6541 tcggcgttat gagcattgcc gcgcctgtat ccgctttgct ggcggcggcg ctccctgttg
     6601 tggttggtat tttccgcgaa ggcctgcccg catggacaac cttcattgga tttggcttcg
     6661 ccctcttcgc tgtgtggatg atctcgcaag gggaaggcgg cgtgaccgac atcttctcgc
     6721 atcttgccga cttgaaactg cctctcattg caggcattgg attcggctca tactttgtct
     6781 tcatgcacga agccaccagc acaggctcca ccatttggcc gatggtcgca tcccgttcgg
     6841 gtgggatgat cttgatcacc atttacatgc tcgtcgtccg cgttgagtgg aaggtggagg
     6901 atcgatccgc gtggccgatc attgtcctca atggcatcct tgatatttct ggcaatgttt
     6961 ttttcatcct cgcaggccaa atggggcgtt tggatgtggc agccgtgttg agttcgcttt
     7021 tccctggaac aacggtcatg ctggcgtgga tcttcctcaa ggaacggctt tcccctaccc
     7081 agtggatcgg gatcgcctcc gcattgaccg cgatcgttct gatgacaata taggacgtga
     7141 aatgaatcca tacctgcttt ttgcgtttat ctttgctttg ctcgaagcgc tggctttgtg
     7201 gaaaggctgg attcgtttgg agtatgttgc caaacctgct gtgatgatcg ttctattgct
     7261 ctggctgtgg acctctgttg gaatcagcgg ggctttgctt tggtttgggg ttgggattct
     7321 tttttcactc cttggcgatg tgttgttgat gatctcgctt gaccgcatgt ttttattggg
     7381 gctggttgct tttctgctgg cgcatatcgc ttatgtgatc ggattcaaca ttcccttgcc
     7441 tgtcttttcg gcgtggagtc tggtctttgc tttcatggtt ggcctgggcg gcacgcggat
     7501 catccgccga atccttgagg caattggaaa caaccgcatg cgcaagcctg tgatcgtcta
     7561 tagtctggtc atctcactga tgctgctttc agccatgatg aaactgaccg atgtcacatg
     7621 ggatgcgggt gcggcagccc tggtcagctt gggtgcattt ttgttctacc tctccgatat
     7681 tgtcctcgcg tggaataaat ttgtggctcc cattcaaaat gggcggattt acaacatcgg
     7741 tttgtatcat ctcggccaga tcgccttgat cgccggggtg atcgcgcaat ttggttaggg
     7801 cgtcttcaag tgagctaaaa gggattcatt tcaaacaaag gcaggcaaaa tgcaatcaat
     7861 tcttgtaatt atcatcatcg gtcttgttgg cggaatggcg gttggtttgc aggctcccat
     7921 gtccagcatg atcagtcagc gtttgggggt acttgaaagt attttcatca ttcaccttgg
     7981 cggcgctctc gtggttttgc ttccgctctt gtattttggc ggtggaaaac tggggcattg
     8041 gcggactgtc ccctggtatg cgctgtgcgc gggggccttt ggcctggtgg taattttttc
     8101 gatgagttac atgattccgc gcattggtat cgccactgcg ttgatcatcc tcctggcagg
     8161 gcagatcctc atcggctcca ttcttgacca tttcggtttg ttgggggccg cagttcgtcc
     8221 attggatttc acccgtgtac ttgggctggg tgtggttctg gcgggggtgt ggctggcgat
     8281 gaaatagacc ccattacttt tgacccatat caccccactc ctgtgatttg tataatcgcg
     8341 gaaaattatt tcacaggagt tttcttttca tgtacaacta tcccctcaaa ttcgaattcc
     8401 cgatgatcag ccttggccgt cagatggacg tcaaaaacgg cgatggcggt tttatcatgc
     8461 gtgtttcgga tccctacatt tcgtttaagg atgaaatgac cgtcattaat ggcaaggggg
     8521 ccgcggttat gaaggcgaag ggtgatagct cttttcgctt tttgttccaa tttgccagca
     8581 cctccaccag ttggaatatt gtggatggtt ctgatagaac gatcgcatgg gtgaccaatt
     8641 attgggcgcg catggaagaa ttcaaggatg tgtacgtcga tacgccttcc ggtgtcggcg
     8701 cagatggacg tgcggatatc ggcggcgtgc ttggtaacat tgccacgaat gtgcttaaca
     8761 atcgggttgc ttcaaccctt cccagcaggc tcgtttataa gatcacagac agcgagtatg
     8821 gaaatgtatt gggttgggtg gtgccttcac gcggtacagg ctggtttgac ttcctgccct
     8881 attcgacccg cattcagatc ctcaacctgc cttttgcggc gcgtgcctat gccccttcct
     8941 atgaattcaa gcttggaaac ttgtatggcc ccactgtgct caaactgcaa aagcagcggg
     9001 atctgttggt ggacaaatac actctggaaa agatcggtga cttaagcgaa gccaatgagc
     9061 gctgggcact tccctccatt gcactggctg ccatgttcga acgtcagcgt gtgaaagaaa
     9121 tggctgattg gtaaatcaac caactacaac ttaaagcaaa gcccgcccac gaaactttcg
     9181 gggcgggctt tttttattga aacagaatca atttacagtt tcgtcagacg cgttgtcttt
     9241 cctattccaa agtcacaatg tatttatgga tcaggtgtga atactgattt tgcaagcgga
     9301 tgaagtgatt gttgatcgca tcagcttcgg caccgtcggg tgcaagaaat ctgccgcgct
     9361 ccacaccgct gcgggcgatc tcgcggcaca cagcttcggt gatcaactct gccaaaagca
     9421 cctggccttg cactgtggtg tcgaggcggt tttgtttatc gaggtagagc tttaccgaag
     9481 gcgcggcggt ggcaatgaca atgcttgaat ttacgcggtc aaaatataca cgctgacgcg
     9541 ggtcggtgcg gtcgtcgaag tggatgtcgg tgaacagggc attgctgctt ctggatggag
     9601 caatggtcag cgtttctttt ttagagcgga cctgtaccaa agcctgtgct ctttgttttc
     9661 caatgtaggc ggtcaccagc ccttcgccgc ccacctgcct gccttccact cgaatatgcg
     9721 cgcgcccgac actgtcatcg gttttgtggg gttgaaggac agcctgttga ttgagcacga
     9781 tgacttcagg gctgttggag atcacagtga ctgttgcacc tggacggacc ttttcagata
     9841 attttacgaa cagggtcaat gaaatggtct gccctgtttg cacatggaca cgctcaggga
     9901 aaaaaccgat tcctgacggc ggcaggtcga ttcgtttttg gtcgccgtca tcgcgcctgt
     9961 cactgagttc attcaaggcg atggtgttca attcgtgcag ggcgtggtct attttttggc
    10021 ggaattgttt atcgagatgg gtttgctcat cttgaacggc gtgtttgcgc tcggcttcaa
    10081 tgagcggttc gagtttgttt tcaacagcca gtttcaacgc cttcgcaaat ggatgcgacc
    10141 agttgatgcc gtcacgagtg gcggtgagga cgggttcatc gttcttcaac agatcgtgca
    10201 gatgatcgca ttggatcgag ccgtagaaat aacccgcata cggatcgttc tcgaacttga
    10261 gcatggtcaa cgaaatgacc gtggctctgc tgatgaccag caggccgcca tcggcataat
    10321 cgccttcctc gcctcgggtg gataactgga tcgcagaacg gaacacttcc agctttgccg
    10381 cggcagggta cccttcgatc ttgatcttct cactgaggac cttctcgcct cgcggtgact
    10441 tgtagctgag tatgtgctcg tgacggatct tctccgctga tggaacatcc ctgaggatga
    10501 ttttgcgctt cgggttgctc atgatcggac gaagctcgaa atgccgctgt agatagttgc
    10561 ggaagttgtc gaactgcggc actttcacat cgtcacgcga gacgatgatt tcaaggatgg
    10621 tgccattgcc ttcggggatc ccatattttt cgcgcagggg aactgtggca cgtacgggcg
    10681 gctgcaattc aaaggtggga attccattct tgagcagcag cgcacaggaa taaaaatatg
    10741 cgcccttgaa cgaatggaca tatccatacc ccagcccgaa aatggaatct ttcaagccgc
    10801 gcccccacat gccgcggaca tttttatctt ccttcaatcc gctggtggct tcgccgtagg
    10861 tgccaacgac tttatccatg cgggtgtcgg tcatgccttc ggcgcggtct gttacgcgga
    10921 tgatggagtt cttgtgcttt cgctggatgt caatgatgat ctcgccgctg accttcagcc
    10981 ctgcgtgttc aagccgcgaa tagctgtcgt tggagttggt gatgatctcc acgagcgcgc
    11041 gcaaaacgtc cttgcgtatc gcctgatcgg cttgttgaag tgctacgcgg tcggcgtact
    11101 gtaatttacg actgcctgcc attaataaaa aacctttctc tcttctcttt acttcttttt
    11161 agcggccttc tttacaggac ctggttttaa caaggtttca aaagaagttt cacaatatcc
    11221 aaggaatgta agtagatcct ggcggttgtg aatgctggca taccctttgt agagttccac
    11281 gttccttgcc agtgtttcgc ttgaagattc catcgtgcat aacagcagac ttctgaaatc
    11341 gaattcgctg atctttgatt ctttgccgtt ctttttgtac aggcgatagg catctgaaac
    11401 ttccatcgca cggacgaatt ttgcagcacg cgccttctcc tcgcgtgaga cagcttgcga
    11461 aacaacaaca gggacgactg ccttttcttc ttttttcttt acaggctctg aaacaacagg
    11521 aacaactgct ttttcttctt tcttctttgc aggtttcaga acaacaggag taacaaccgc
    11581 aacttctttc ttcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncttct
    11701 ttcttcttta caggcttcga agttgcaaca ggcgcaacaa ccgcagcttc tttcttcttc
    11761 acaggttccg aaacaacaac aggagcaacg accgccttcg tttccaacaa tgtaagttga
    11821 actgcttgct gtgcgatcac tttctcagca ggtttctgag gcaggcctgg tttttgtact
    11881 ttgaaaattc caagcgcttt ggcagctcgc tcggcagcca gagtcccttt tacggtgagt
    11941 ttgaatccga attctgtgtt tgcggcgatg taccccttgc tgcggcaatc gcccacccgc
    12001 ctgccaacca cggcagaatc aggccagcgc ggatattttt tgagggcaaa tttatggggg
    12061 aacagcagaa aacaaccaaa aaccacatcc tccatatgga tttcgatgcc gcgttcaagc
    12121 agaaaatgaa tcgcatacgc aacaaggttt acggatgaga tggcggaata aacgctcgga
    12181 tcgaatttcg gcaggcttct tctctttagc ttgcgtgcgc cagaggtttt ggctgtcatc
    12241 agggtttggt tctctttcgt catatggctg tgcggcagta tattacacca ttttccacaa
    12301 aatgcagtca gcggacaggt tatctgtaaa tggtatcata tggttagagc cgcgaaagga
    12361 tacccttatg atgaataacc gtcaacttgc cgatacgttt accctgatcg ccaatttatg
    12421 tgagatcaaa ggcgaggtca tttatgtcat tctcgcctat cgcaaagcct ccgaaaattt
    12481 aatgaccctc gggcgtgaag ccagcgaata ttggaaagag gggaaactgc gcgagatccc
    12541 cggcgtgggc aaagccatcg cagaaaaaat agatgagctg ctcaccacgg gcaaactgca
    12601 attccttgaa aacctcaaag aggaagtccc tgcttccttg gcagattggc tgcaagtgcc
    12661 cgggctggga ccgaaaaaga tcgccctgat ctggaagacg ttaaacatca ccgctctttc
    12721 tgaactggaa actgccgcca aaaacggaca gcttcgcgac ctcccgggca tgggcgccaa
    12781 gtccgagagc gccattctgg aaggcattgc atccctcgcc cgcaggtccg ggcggattcc
    12841 gcttgggcgg gcgtatccgc tggcgcagga gatcatcaga actctgaagc gggtggatgg
    12901 agtcgtcgca gcagaatccg ctggcagttt aagaaggatg cgttccacgg tcggcgatct
    12961 cgatattctg gtggcatcca caaattccgc ggcggtgatg gaagcgttca ccaatctacc
    13021 gggcgtgagt cgtgtgcttg gcaaaggcga aacaaaatcg agcatcgaat ttaccgatgg
    13081 agtccgcgcg caggtttggg tgcatgcgcc cgaaaagttc ggcacggcgc tgcaatatgc
    13141 cacaggctcg aaagatcaca acgtgcagct gcgtcaaatt gctttggcta aggggctgtc
    13201 gctttcggag cactcgctga caaagaccaa tggcaaggga gaaattttct gcgccaccga
    13261 agaggaagtg tacgagagac ttggcttgcc gtggattccg cctgagttgc gcgaagatca
    13321 cggggaagtg gtggcggcaa aggcaggcag cctgccgaag ttgattcagg tcaaagacat
    13381 caaagccgat ctgcaaatgc actcgaattg gagcgatgga aaattaacca tgctcgagat
    13441 ggcgcaggcg gcgatcgcac atggtttgaa ggtcatcgcc ttcacagacc attctgccag
    13501 cctcggcgtg acgggcggct tgaaaatgga agaccacaaa aagcaagcgg cagagatcaa
    13561 gaaggtccaa aaacaactcg gcgacaagat cctgattttg cacgcaagcg aagtggagat
    13621 caaagccgat ggcacgctcg attatcccga tgagtttctc gcttcgctcg atctggtgct
    13681 ggcttcgatg cacacctctc tgcgtcagcc gcgcgagaaa gtgaccgagc gcatgatcgc
    13741 cgcgattcgc aatccgcatg tggatatcat cggtcatccc acgggacggc tgattcccga
    13801 ccgcgaaggc gccgacctcg atatggaagc cgtgctcaat gccgccgctg agacaggcgt
    13861 ggcgctcgag atcaacgcgc atccctcgcg cctcgacctc gatgacatgt atgcccgccg
    13921 cgcaaaggaa ttgggcatcc ccatcagcat caacaccgat tcacattccg ctgaagattt
    13981 cgacaatctc ttttacgggg ttgccacggc gcgccgcgcc tggctcacga aggaagatgt
    14041 cattaataca tggtccacga aaaagctgtt gaattggctg gagaagcgcg gaaaatgaaa
    14101 gccctgctca agccttcgtt aacgaagata ttgctcgctc tgggtttatt tatcgttgcc
    14161 agttttttgt ggaaatatac ctttggcctt gtcatcatgg atgtgaacta ttttggatgg
    14221 cccttgcatt acttgacaac ctggggacca tgcagggcag gggagaattg ttccgaattc
    14281 aacgggatgt acctggtttt cgatctcctg ttttggtata tgattggcgc atggatcgtg
    14341 aatcggtttt cgaaaaaaac aacctagcat tggaggcaga atggcagaaa gtttaatacc
    14401 ggcaaatgaa gtccgctttt ttagcgggag ttcaaatccg caattggcgg ggaagattgc
    14461 actgcatttg ggggtgccgc tggaagatac tcacatctca cgtttcagca acgataatct
    14521 ttatatccaa ctgggggcca gtgtgcgcta tcgccgtgtg tacatcgtgc aatcgctctc
    14581 aaagccggtc aacgatcatt tgatggaatt gatgatgatg atcgatattg cacgcagtgc
    14641 agcggcttcc gaggtccatg ccatcattcc ctatttttcc tttggccgtt ccgataaaaa
    14701 agacgcgccc cgcatttcca tcaccgcccg ccttgtggca gatctattaa agaccgcagg
    14761 agccacgcat gtgatgagca tcatgttcca ctcaccacag gtgcatggtt tcttcggcat
    14821 ccctactgac ccgctaagca gcagcaaggt tttcaaagaa tatctttcaa aatccgacct
    14881 gagcggctcg atccttgttg cgccggatat ggggcaggcc aagaccgccg cccgcttcgc
    14941 gcgtactctt aacctgccca ttgcggcagg caataaagag cgcgtttccg ataccaaggt
    15001 catcatcaat ggattggtgg gcgatcaggt acgcggtcat gaacgtgccc tcatctacga
    15061 cgatgagatc gccacaggtg gctccatcct tgaattaagc cgtgtcttga tcagcgaagg
    15121 actcaaagac atccgcgtga tctgcaccca tggtgtcttc agcaacaatg gactcgagcg
    15181 tctggctgaa gtccctgaga tcaccgagat catcaccaca gatactgtcc atatcgaacc
    15241 tgagcgagtc cacccgaagt tgaccgtgct ttccgtggcg cctgtctttg ccaacgccat
    15301 tcgtcacaac atcaatcgcg aatcgcttag cgaccttttc gtgtacggcg aatannnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcgac gggaaaacta cctctgcctg
    15481 tcgaaagtgg atcggccccc tgaaattagt tttctttaac ctgaaagatg tatactgtaa
    15541 tcaaggaggc acgacatgat aaacaaaacg atgcaagatg caatgaacga ccagatcaac
    15601 aaggagttat tctcctctta cttatatctc tccatggcgg cttattttga agataagaac
    15661 ctgcctggct ttgcccattg gatgcgcata caggaagccg aagaacgcga acatgccatg
    15721 aagttttacg atttcatcct tgagcgcggc ggacgggtga tgttgaaagc catcgaagct
    15781 ccaaagaccg attggaaatc agctttggaa gttgcggaag aggtggcggc gcatgaggcc
    15841 aaagtcacag cctcgatcca tgctttgtat gagttggcgc taaaagagaa ggattatcct
    15901 gcccaggtga tgttgcaatg gttcatctct gaacaggtgg aagaagagaa gaatgccgct
    15961 gaaattgtgg caaacctcaa gttgatcgag gaacgcggca ccgccgtatt gatgctcgat
    16021 caccgcctcg gaaagcgcgg cggcgagtag ttcgaatccg cccctaaaga ctgtctggcc
    16081 tttaggggct ttttattttt ggagacccat gcaaatcacg attcgtcgtg ccacccatgc
    16141 cgataaaggg gaatggtttc aaatgcgcaa gggcatctgg cccgatgtgc ccgatcatta
    16201 tatgagtttt gatatggatg acctgcttgc cagcgagaat tatctggtgt tatttgcctg
    16261 tgatggggat actcccattg gcctgaccga agcgagatta agagaccatg ctgaaggatg
    16321 cagcaccagc ccggttggat acattgaaag ctggtatgta cgggaggatt accgcggcag
    16381 gggagtgacc ggaataatga atcaggcggc tgaagagtgg gcccgctcaa aaggctgcac
    16441 cgaaatggct tccgatacct ggcttgataa cgaagccagc atccgcgcgc acttgaaact
    16501 gggatatttc gaggtggagc ggctggtcca ttttgtgaaa aaactgtagc gggtgcccgt
    16561 atttgtggtg aaatcacttg tgggaaaaaa gtccttgccg cgacgggcgt gcgaggatgt
    16621 aaaatagagg aaactgccgc taaaacaaga tgcaggtaaa cggtcgggtt ggatgcgtga
    16681 agagtgggga aatcctgctc tggattgtag tcatcttgca gtttggcatc tgaatggttc
    16741 ctgttccaat aaatctgtat aatgattgcg tgaccttgta aattttatga gcagcatacg
    16801 tcttagagtc gtcattccgt acctgttgtt gaccctgatc gtttccggga tcggggtgta
    16861 tgtcgtcacg aatctcgttt ccaatacgtt gagcgagcgt ctggcaaatc agttggtgca
    16921 atcaggccgt gtggtgttgg atggttttgt aaaacaggaa cagtctcaga tcgaggatgc
    16981 gcgttttatt gcctttacct cgggtgtggc ggatgcgctt gtaagcggta cggctgacgg
    17041 gcttttggtc gaacaggcca ccacgcaaat gacagggcgt gatattgata cgcttgtgtt
    17101 catttcatcg caagggaatg agatcttgca tttatggaag aacgacctga atgtggttca
    17161 gcgggccaat gaaaattcgg gcatggctgg ttcgccgctg gtcacgccgt ttttagccag
    17221 cagaaacacc cgcgataatc cacggcactt tttggggacc aactcgctga atgaccgcac
    17281 ttatttttat gcttccattc ccgcttcgtt gaatggtgaa tttgcgggtg tggtggtgat
    17341 cggtatcgat attgagaagt tgctgccgaa tttaaaaagc tcggctttgg ccgatgtggt
    17401 tgtctacggc gcggatggga gtgtcattgc caccacgctt gatgtcagcg accctttgag
    17461 tttggaggcg ctttcgatca ccaacgataa ttaccagcgc atcagcgccc tgaccgatct
    17521 ggtgacgggg agcaacattt cgattgacag gcgttcgtat agtgtggcgc gtggttcttt
    17581 gcaattgggg aacgataaat tgggggtgtt tgctgtggcg ttgccgttgg attttgtcaa
    17641 tcaggcggtt tctgaaaatc agaccaccta cacacttttg ttcatcggcg ttttcttttt
    17701 cgtaattgtg atcggtcttt gggtatcgcg ctcgatcgtg aatccgttgt atcaattggt
    17761 ggaaacttcg caggccattt ctgcgggtga cctcgaaaaa cgcacgggca tcaaatccag
    17821 ggatgagatc ggtattctcg cctcggcttt tgatgagatg accgcgaacc tgcaggagcg
    17881 caacgcccaa ctggaacgtg caaaccagat tttgaagaat attgataaaa cgaagtccaa
    17941 tttcattcag atctccgccc atgagttgcg tactccattg acgcttatca tggggtattc
    18001 acaaatgctg gagcaggatg cccgcaacga cccagaatta aagtcactgg cgcaggggat
    18061 tttagacggt tccgaacgca tgaccagcat tgtggaaagc atgctggatg tttcccgcat
    18121 tgacagcaat tcccttttcc ttagaaagac cggcctgcgg attgacctga tcgttcggaa
    18181 ggtttacaaa aattttgaaa aggccttggt cgagcgagat ttgcaatttg acatgaacaa
    18241 cttgcgggat ctgccgctta tccctgccga cccggatatg ctgcaaaaag tgttttatca
    18301 tgtcattatg aatgcgatca aattcacccc ggatggcggt tccattacga taagtggacg
    18361 gtatgtgccc gccaatgagc ccagtgtgga aatttccatt cgcgatacgg gtatcggcat
    18421 caaccctgaa atgcattccc tgatctttga gaaattcaat cagacgggtg acgtactttt
    18481 gcactcttcc gggaagacaa aattcaaagg cggcggaccg ggcctgggct tggcaattgc
    18541 gcgtggtatc gtagaggcgc acggcggccg catttgggtt gaaagcccgg gctataacga
    18601 agaaaccaat ccaggcagta cattccatat ttccctgcct gctcccgaag accaacaaga
    18661 ggaggtgcgc cgtgaaccag cgatctccaa acaatagacg tgaccttaac ttatttgcga
    18721 tcattatctc cattggagtg gctttgatga tcggtgccgg acaactggca gcgcggctcc
    18781 catcgacctg gagcctgtct gccaatatgc gctccaacct taccccaaac tttttctcgc
    18841 gacgacctga gaagactctg cctgctgtgg aagaaggtgc gggcgaaaag caggatgata
    18901 cttatcttac gcccggcact ggcactgctt ctggttcttc ttccgggccg atcaatccga
    18961 acggttcggc aaccttcacc caacgcccgc ccgctactgc cacttccacc cagcgttctg
    19021 ctccgacagc cacacgtact tctccgccca ctgcaaccag tacgagacgt tccacagtcg
    19081 tggctgttac atccacgcgg actgcaacac gtaccccgat tcggacttcc acctccgtgt
    19141 ctcgaacccc aaccatgacg gccactcgta ccgccacatc tttatttact gccaccagaa
    19201 ccgccacccc aacaaggaca gccacttcaa cttcgacagc aaccgttacg cctacgtcaa
    19261 cgcccactgc ttctgccaca gccacattta cggtcatacc gaccgctaca aatacggcta
    19321 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngttccaata cccttcccgt
    19441 aaaaaatgaa aaaacattaa tcaatacaaa ctcactccga atttcatttt cgcgagtgag
    19501 tttgtcatta agccctgatt tttcgttttt cttttcaaaa atcggcggtt tggctgaacc
    19561 ttgcatggag tctgctctca taaaacccta atacgttggc agtaactata tcggttgcac
    19621 agcgaccaat aggataagac caacgatttg agtataaaag gagatttctc atgcgaaaaa
    19681 gtactttgtt catttcagcc atgttgaccg tattcctcat ggctacctta tttggggtga
    19741 tttcagccta tcagaacatc atggcaacca atcaggctgt agtcgtaacg cctgctgagt
    19801 tatcggttca ggaaccggct gccacagccg tgccgacgat cgctgcaccc atggtgatct
    19861 caccggagca agccaccgcc attgccgtgg aattgctcgg agataccaac gtgtatagtg
    19921 ttgaagtggt ggattatgag ggcgtggccg cctatctggt caccttcagc gcgggtgacc
    19981 ttgtgtatgt cagctccacc ggagaagtcc ttgctgtgac caaattggaa cctgttgtta
    20041 tggtggtccc ggccggtaat aggaacgaca gcggtgaaaa gcccaaccgt gaccagcaac
    20101 aacaggaaga tgatggtgac gatgacgacc atggtgaaga tgaacacgaa gatggcgatg
    20161 attaatcagg tacatgcaca tgaatccaaa acccgcccct cgtaaatgga ccgacataca
    20221 actggcgatc gccgcagtct caatgacgtc tgtgcttgct ttttggaata tgtttgccgg
    20281 gccagatcgc tcaaaatctg aagaaaaagc tgctgaggcg caacaggctt ctcaagctgc
    20341 acccacctct gcggttatgt ccgtttctcc cgcccctacc atgcccccgg ctggatatac
    20401 cattttattt ggcggcacag ccccccagcc tcaggtcatc gtcatgcaat cggggcaggg
    20461 cggtaatcag ggcggcggcg atccggtcac tgtcaccaca tcatcataag catgttgcac
    20521 cgattttcct ttcgcgcaat gggttcagat atcctggtca ttttggaaca ggattctgaa
    20581 gccaagcccg caattctgaa agacatccct gcatggtttg aaaactggga acagaccctc
    20641 agccgcttca gaccgacctc ggaactgtca caccttaacc gtacatttga ccagccgata
    20701 aaggtcagtg atgcattttg ggatgtgttt caaactgcat tatgggccga tgagatcagc
    20761 ggcgggttgg tcaccccaac cgttctggat tcaatggtcg aagctggcta cgatcgtacg
    20821 tttgacaaat taccccgcag tcaattcgat atgatcgcat cacccgccga ggcagatcac
    20881 ccactctcca cggtaatttc agattgtgac gccaaaacca ttaaccttcc aaaagggact
    20941 ggtctcgatt ttggcggcgt ggccaaaggc tgggcggcac atcaggcagt ggaacgcctg
    21001 caagaattcg ggccatgtct tgtaaatgca ggcggtgata tcgccgttag cggtccacgc
    21061 atggatggga gtccctggcc tgtcggggtg tcgaacccgt ttaacgtcgg agcaaacata
    21121 aacgtcctgc aagtgaaaca aggcggcatc gccacctctg gaaaagaccg ccgcaactgg
    21181 aatcgcaatg gcatttttta tcaccatatc atcaatccac tcaccggcct gcccgctgaa
    21241 actgacttgc tgcgggtaac cgttgttgcc cccacaacca tgcaagccga agccgccgca
    21301 aagacagcct tcattcttgg ccaggaaaag ggactgcatt ggattgaggc acaacccgag
    21361 tttgccgggc tgctggttct tgagaccggc gacgttattc atagccgccg tatgcaaaag
    21421 tatttgtaag gagtctgata tccatgaacg cacaacccca aagtgaattt aacgagtctt
    21481 cggtcaacat ccagtcattt ttgttgtttc tttttgcaac ggtcattggc ctgttggtgg
    21541 ctgtattgtt gttacccatc ttcctgccaa acatggcttt ttccttgagc ggagacagcc
    21601 cgaaagctta ttggtatctt tcgcgcgcca cagcctttgt ctctctttcc ctgttatggc
    21661 tttccatggc gctggggttg ggcatcacca ataaaatggc gcgcttatgg ccgggcatgc
    21721 ccgcaacctt tgccatccat gaatatgtaa gcctgctggg gttggccttt gcgatcttcc
    21781 atgctcttgt gattttagga gatcactata tcaacttcac cttgctgcaa ctgtttatcc
    21841 ctttcagcac tgtggagtat cgcccatttt gggtgggggt tgggcagatc gggttttaca
    21901 tctgggccat tgttgcattg agtttttaca tccgccctgt catcgggcaa aaattctggc
    21961 gttttttgca ctatgccagc tttggcatgt atggaatggg gatcctgcat ggcatcttca
    22021 gcggcaccga caccagcctg gcgtgggcac aaaactatta ttggtattca gcaggaagcc
    22081 ttatttttct cttctttgtc cgcattgttg gaagtttgat ggatgtcctc ttccccgcca
    22141 aaaagcagac tcccgctgcg cgtccgactc aggggtaact gcttctgttt tctgaaacat
    22201 tcgctacgaa ataaaaagag cggccaattc aattggccgc tcttttgcat tgttcccttt
    22261 attcttttac ttcaccgtcg atcacatccc cgttggcatg accgctcggt ccactggggg
    22321 cggggccgcc ttcaggctgc ggttgaccct gtgcatacat ttgctgactg agtgcatgga
    22381 aggtgttttg caattcttcc gtgagctttt tgatttggtc cacatcatca ccttgtgccg
    22441 cctgcttcaa ctggttgacc ttggcttcaa tggcatcacg ttctgccgcg ggaaccttgt
    22501 cgcccagatc gcgcagtgat ttttcgattt gataggacag gttgtcggcc gtgttcttta
    22561 cttcaatcac ttcacggcgt tttttgtccg cagcttcatt ctggcgtgct tcctgcacca
    22621 tgcggtcaat gtcattcttg ttcaggtttg tcgaggcggt gatggtcacc ttctgttcct
    22681 tgcctgaagc cttatccttc gcggtgacgt gcagaatacc gttggcatca atgtcgaagg
    22741 tcacttccac ctgcgggata ccgcgcggcg ccggtgggat tccatcgaga cggaagcggc
    22801 caaggctcat attgtcgttt gccattgggc gttcaccctg caacacatgg atatccacag
    22861 cggtttggtt atctgccgcc gttgaataca cttccgtttt gcggaccggg atggtggtgt
    22921 tgcgttcgat cattacggtc attacacttc ccatcgtttc cacaccgagg gaaagcgggg
    22981 tcacgtcaag caggaggata tccttcactt cgccgccgag cacaccgccc tgaatggctg
    23041 cgccgatcgc aaccacttca tcgggattca cacccttgtt gggatccttg ccgttggtta
    23101 acttgcggac caggtcttgc accatgggca tgcggctcga accgccaacc agaacaactt
    23161 catccagttt gcctgcatcc atgcctgcat ctttcaacgc ggcttcaaag ggcttgcggc
    23221 agcgctgcaa caggtcttcg gtcatctgct cgaactttgc gcggctgagt ttcagctgca
    23281 tgtgcttggg cccgcttgca tcggcagtga tgtagggaag gttgatttcc gtctccatca
    23341 tggtcgagag ttcgatcttt gctttttctg cggcttcacg caaacgctgt aaggcctgac
    23401 ggtcctggcg caggtcaatg ccctgatctt tcttgaattc gtctgcggcc cagttggtga
    23461 tcttctgatc ccagtcatcg ccgccgaggt gggtgtcacc attggtggct ttcacttcaa
    23521 tgacaccttc acccacttca aggatggaga cgtcgaatgt accgccgccg aggtcgaaga
    23581 cgaggatggt ttcattcttt ttcttgtcga ggccataagc aagtgcagag gctgtcggct
    23641 cgttgatgat gcgcaacact tccaagcctg cgatcttgcc tgcatctttg gtggcctggc
    23701 gctgactgtc attgaaatag gcagggactg tgatgacggc ctgtgtgaca ggttcaccca
    23761 gatatgcttc tgcatctgcc ttgagcttgc ccagaaccat ggcgctgatc tcctgtgggg
    23821 agtattccct gcctgttaca ggaattttga cgcgcacatc gcccgtgggg ccttgaatga
    23881 cctgataggg aaccatgctg cgctcggtgc tggtctcttc gaaatgacgt ccgatgaatc
    23941 tcttgatgga atagatcgta ttatctgcat ttacaaccgc ctggcgtttt gcggtttgcc
    24001 ccaccatgcg ttccccattt ttattgaatg ccacaacgga tggacataat cgtccacctt
    24061 ccgcagtggt gatgacagtg gggcttccgc cttccatgac ggagaccaca ctgtttgtcg
    24121 tgccaaggtc aatgccaatg atttttccca tgttatagaa ctccttacat tatctaagtc
    24181 gaaggcaggc gtatttttta cggctgtctt ttattggtgt gcagttatct taaacgactt
    24241 gtacaagaat tctgttaacg ctgtgtagaa atttcatcgg caggaaaggt atttattcgg
    24301 gttggctgat tttgaaccag tcgaacatga cgatggcggg tgcttcttca aaggaggctg
    24361 cagtaatgcc aaccttgccg ttgaccagcg ggtagcgggc tacatcaagt ttgcggaaca
    24421 tttttccatt ggcgtgcaaa agcaggaagg tcccctgaca ggtgaggccg atctcgtaat
    24481 ccatgcctgg ggtgatgtat tccacgctgg cattgacgat tggggtgtaa ctggcgatcc
    24541 cgtctgccag ccattgcccg agcaggacgc tgtaggtgcc atcactgttg atgttgtatt
    24601 cgaaccagcc gtcttcgctg tagcggcaga taacacctgc agaccctgcc ggtgtgccgc
    24661 tgaactttgc cgtgatggag acatcctgat attcgtgtga gttatggatg gcgtagtacc
    24721 aggtgtttgg cgagaatagg tcgagccgta gtctatcgtc tacgatttca agcgatgggc
    24781 tgagctcacc cccagtttgg aaggaggacc aggcggtcat gtcggtgttg tattcttctg
    24841 tgaaatacat ggggaccggt gtggcggtta atgttggggt gggggtaaat gtcgcgaagt
    24901 gtgtcgctgt ggcagg
//
LOCUS       DAOQLC010000064        24683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_672_length_24683_cov_22.463091, whole genome shotgun sequence.
ACCESSION   DAOQLC010000064 DAOQLC010000000
VERSION     DAOQLC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_672_length_24683_cov_22.463091"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PL000_12340"
     CDS             <1..246
                     /locus_tag="PL000_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HNC89736.1"
                     /translation="PVIGNVHAAPLADAEAARADIIAQMQSRVRWTESVQYMAANGVN
                     TFVEVGTGSVLGGLVKRIADGAGIYPLGNPQDFAALE"
     gene            332..1588
                     /locus_tag="PL000_12345"
     CDS             332..1588
                     /locus_tag="PL000_12345"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNC89737.1"
                     /translation="MKKILLPFILITLIVAACEPLPTTPQFVTQIPFYASQTPEFIIP
                     VTDTPIVSEPFTETPAFIVPVTETPTPEFIIPVPETPTPVPLAGWWEVYFTDPLAINN
                     PNAFAGSIEEKLIQWINAAQVSIHIASFEFNLTPVAEALIAAKNRGVDVKWITDDEYG
                     LKIDSEPERRQFSLLMGAGIEVKDDAGREAFMHNKFWIFDQQAVWTGSTNITVNGIFK
                     QNNNVIVLRSPEIAYIYEREFQELWSGQFGPRAPSTISNQWAILDTTPVQVLFSSEDN
                     AMNSLLAVVNDAKFSIRFLAFSFTDDALAQTMIERFKAGVDVQGVYETLASTSIYSDL
                     KTFWCAGVPVRQDGNGSLMHNKVIIVDNSTVITGSLNFTAGANEKNEENVIIIDNPEI
                     AALYLQEYAKIWSLAHDVEAGTFTCQ"
     gene            1844..3061
                     /locus_tag="PL000_12350"
     CDS             1844..3061
                     /locus_tag="PL000_12350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC89738.1"
                     /translation="MKLSKLALEIAESPTLALNEEARLLRERGEAVINLGIGEPKNKT
                     PIAAILASGAKLSSGEVKYTPPDGLPSMKKAVIRYTEENYNRLVAPENVIITNGAKQS
                     LFNIFYSILNPQDEVIIIAPFWVSYPEMIKMCLGVPVVVTPEDGTFTPRFEDIERAIT
                     SSTRAIVVNSPNNPSGAIYPPELVEKIVELCERKGIFMICDDIYHKLVFGRNVAVPAY
                     SFTKKEIDNSHIIVVNGVAKVYGMTGFRIGWVIAPRDLIKVMTNVLAQTTSCVSPIAQ
                     AAAEGALNGLQSVVEALRLQIQNNREVVLQEMRTFNGARLIEPKGTFYALLDLRAFNT
                     NSVELSKFLLKKALVVTVPGREFGMEGHIRISFAGSIKDITEGIARIKWALDPTSPNE
                     IYIGDKKMIRDWM"
     gene            3156..4802
                     /gene="pckA"
                     /locus_tag="PL000_12355"
     CDS             3156..4802
                     /gene="pckA"
                     /locus_tag="PL000_12355"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNC89739.1"
                     /translation="MNNLLNIKTPAESIAQTRKADYNLSNNGIGNLRLAYWNLPTEAL
                     VEEAIFRNEGALVAGGPFVANTGKHTARSANDKFVVRHTDSENNIWWGVYNRPFEADK
                     FEVLYDRMLGFLQGRDVFVQDVYGGADESYRLPVRIVTELAWHSHFVRNMFILPQSLE
                     EYKRFVPEFTIIAMPSFKGAPAVDSTNSETFICLSFEKKLAIIGNTAYAGEIKKSVFT
                     ILNYLLPLDGVLSMHCSANVNPDNADDVALFFGLSGTGKTTLSADPTRRLIGDDEHGW
                     SDNGVFNFEGGCYAKVIGLSESAEPEIYATTKRFGTILENVPFDPVTRSIDLDDDTIT
                     ENTRASYPLEFIDNAVPEKKAGHPKNVILLTCDASGVMPPIARLTPNQALYQFISGYT
                     SKIAGTEVGLGKEPEITFSACFGGPFMVHHPYKYAELLKNKIERYGVTCWLVNTGWVG
                     GPYGVGKRISIKYTRALLNAALNGKLDQVKFTHDPIFGFEVPTQCPDVPAEVLNPASS
                     WHDQKEYDRRYKDLAMRFKQNFGKFEIGTPQDVIEAGPKV"
     gene            complement(4866..7298)
                     /locus_tag="PL000_12360"
     CDS             complement(4866..7298)
                     /locus_tag="PL000_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNC89740.1"
                     /translation="MHDQIQTRLLRWAGIFLLIQSTILTLAPAVRERSWDVDYRLSHW
                     VGLIAWAVFMTAAHRASIKHLPERDPYLLPAAALLSGWGLLTIWRLDETFGIRQTIWL
                     GLSVTGFILAMSLPQNLFFLRRYKYVLLGGGLLITALTLLLGTNPLGVGPRLWLGCCG
                     VYFQPSEPLKLLLVIYLSAYLADRINIRSSSLSLLVPTLVVTGVALLLLLVQRDLGTA
                     SIFICIFTVIIFLSTGRRRILFGAVFFLALALFLGYFFIDIIRIRVEGWLDPWADPSG
                     QSYQIVQSLLAVANGGTIGRGPGLGSPLLVPVAISDFIYAAIAEETGLAGTIGLLGTI
                     WLLLARGMISSLRAADRFRRYLAAGVVTYLGIQSLLIIGGNLRLVPLTGVTLPFVSYG
                     GSSLLTSFIALFILLTISNLEDSEPAQLQDPKPYTFLAGLLALGLATSALTTAWWAVI
                     RAPDLLNRTDNPRRAIADRYVPRGELVDRDNQPINITEGESGTYQRVYVYTELAPITG
                     YTHPIYGQAGLEATLDDYLRGLQGNPASLLWWDRLVYGTPPPGLDVRLSLSLALQSKA
                     DQLLGGHSGAILLMNAETGEILAMASHPTYDPNKLDEEGEALSQATNSAAPLVNRATQ
                     GLYPIGTAMLPLIRANFGETQPTDNELKAYYQKLGLYQAPQINMPIAYDTENPSAYEL
                     RVSPLQISLAASVLSNHGMMVVPSIATAVNTPEQGWVVLSTAESRPVEMIQAGAADEA
                     ALSFILEGKPFWSHVGQASGSNDTVVTWFLGGTLPSWSGAPLTVVVTLEENNTFLAKY
                     IGENLLDTATSR"
     gene            complement(7501..8370)
                     /gene="mtnP"
                     /locus_tag="PL000_12365"
     CDS             complement(7501..8370)
                     /gene="mtnP"
                     /locus_tag="PL000_12365"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNC89741.1"
                     /translation="MNMEKASIALIGGSGLYQMAGLQNTTEHEIDTPFGKPSSPIVIG
                     TLEGTRVAFLARHGIGHHITPTEVPYRANIYALKLLGAERIISISACGSLREDYAPGD
                     IVIPDNIYDNTKDRVRSFFGNGFVAHVGVADPFCTDLSNDLEAAVRATNAKTHRGGTL
                     ITIEGPRFSTKAESNAFRAWGMSIIGMTASPEAFLAREAEICYATMAHVTDYDVWHES
                     EEPVTVEMVIQTLNKNTQKAQEAIHNLIRGLKEQRNCKCGEALATALITNPKVIPAET
                     RQNLDLLVGKYMK"
     gene            complement(8367..8837)
                     /locus_tag="PL000_12370"
     CDS             complement(8367..8837)
                     /locus_tag="PL000_12370"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC89742.1"
                     /translation="MSAPIILALRLMMALALYGFLGWALFVLWRDVQKQGFSLANRRV
                     PGISLTVRHGRGAPIVKSFTQPEITIGRDPGCDIPLIDDTISTRHAQLTYHHGQWWLD
                     DLASTNGTALNNIMVTMPTVLTSGDEIQCGDTRLIINLSTDTFVSPTQRVERHQ"
     gene            complement(8834..9652)
                     /locus_tag="PL000_12375"
     CDS             complement(8834..9652)
                     /locus_tag="PL000_12375"
                     /inference="COORDINATES: protein motif:HMM:NF023818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HNC89743.1"
                     /translation="MNLKELEARLQALIEVDLLKILPSRKVEDVIVQKLAAAIQLNTT
                     TATDGSVIAPNVYTLIVHPSEAAKWQDQQLLTILLHSISAVAQEAGFKVTISPTITIS
                     TDEKLPLNDAQVVASYRIEPMADTNATPLDTGNLEDYSKIPENAFLIVEGVKVFPLKL
                     PVINIGRRLDNQLIIDDPRVSRNHAQLRAIKGRFVVFDLNSTGGTFVNGQRASQVVLY
                     PGDVISLAGVALIFGQDNPPPRPDLKDTAPFQSATADRPTASLKEHSTAKLKRR"
     gene            complement(9675..10259)
                     /locus_tag="PL000_12380"
     CDS             complement(9675..10259)
                     /locus_tag="PL000_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC89744.1"
                     /translation="MERKIFHGNLKPVDVAQALLGEFNQGNLQAQSFGQSDKMIVQVG
                     THPGAMSGGQTAMTVTIQKHEDGIMVELGQQAWLGVAASLGMSALAALRNPFSLLGRL
                     DDIAQDIENLQMSEKIWQVINQTARAAGASTELSDRLKRLTCEYCGTANVVGDPSCVA
                     CGAPLGKVQPRTCRHCGYVARPDDKKCQNCGQDL"
     gene            10310..10921
                     /locus_tag="PL000_12385"
     CDS             10310..10921
                     /locus_tag="PL000_12385"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC89745.1"
                     /translation="MKRIWFHPTLLIIFTTLFSLWTIFFASSSTVVLAQQPTETPAST
                     GMFITVITDEPQINVRMGPSSSIYPIVGTLLRGSTAPALGRSAGGDWVQIEFIGAPYN
                     KGWVYSPLVQVSPGTLLVVEAPPTPVPPPTSTVDPTLAAQFSIVPTSTRLPTFTPPPS
                     LVVPSYTQAPPVISSQTGSLAIIIIALAVLGMIGFLLSLFLRR"
     gene            10926..11561
                     /locus_tag="PL000_12390"
     CDS             10926..11561
                     /locus_tag="PL000_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89746.1"
                     /translation="MIFARRIRLTAFILVMFLGMVFAFHSPVPTTVHAQQPTGSIPTV
                     TGTPGGATVALYLDEGHPQEYVYSGPSIYLYPAIGVLLAGQEVPAYGYSEDKNWIQIY
                     YPGVPNSVAWVYAPYVKVVKFGELPLVAAPPTPTPASTPVVNINPTLVAAFSTPVLPT
                     RLPTFTPPVPLVVATFVDEAVNTNRVPVGLLIFGFGFVGALGTIISFLRGR"
     gene            complement(11621..12034)
                     /locus_tag="PL000_12395"
     CDS             complement(11621..12034)
                     /locus_tag="PL000_12395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HNC89747.1"
                     /translation="MLQEIETQLFTLTPAASQAVKDILQQRNLEGYALRVYVAGGGCC
                     GVNFGMALDNNFRSVDTTFEANGVKVVVDEVSIDYLRDATVDFVNDPHHGQGFAVSSP
                     NAKGHSHGEGGCACSTNSGGSSCACGGGGACGCSN"
     gene            12230..14584
                     /locus_tag="PL000_12400"
     CDS             12230..14584
                     /locus_tag="PL000_12400"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNC89748.1"
                     /translation="MYYMQGLTSLEAIAIWVVFGIAIIGLLYALFLRSQILREDKGTQ
                     KMQEVWGAIKDGADAYLRKQFRSILPLIGILTIALFLSVYVVPPSPEARERFSTMTES
                     QVRFWIGLARALAFIMGAGFSLTVGQLGMRMAVEGNVRVAAASKKSFGDALRVAYRAG
                     TITGMLTDGLGLLGGTIIFIILGIAAPDALLGFGFGGTLLALFMRVGGGIFTKAADVG
                     ADLVGKVEAGIPEDDPRNPAVIADLVGDNVGDCAGMAADIFESYEVTIVSGLILGLAL
                     WHTTGRLEWIIFPLMVRGIGVLSSIIGTYFVKGGETGKSEDAMKAIFSGFITSAGISA
                     ALFFIAGFFYMKDTMVDYGGWWRMPMAVGVGVLLAILIDRLTEYFTGTHASPVNDIKK
                     AADTGPATLILNGISVGFESSVWSVLVIALTIIASIFIFGTIPGVTGAERATFILYGV
                     AMTGIGMLTLTGNNVAMDSFGPIADNANGIGEMSWSGMEDKETKKAQQIMADLDAVGN
                     TTKAITKGVAIGSAVIAAVSLFGSFLVDVSRAQESLGIPVDQQIQAIGIRVDIPQIFV
                     GMLIGGALPWLFSSFAIQAVTRAASLIVLEVRRQFKLGVLDGKVKPDYAQAVNISTAA
                     AQKELVSLALLGIVTPIVVGLFLQVEALGGFLAGIIVSGQLLAVFLNNSGGAWDNAKK
                     LIEDEPKDPAKNLGKGSERHKAGVVGDTVGDPFKDTAGPALNPMIKVVNLVAVIIAPI
                     IVQYSTATGGVLFVVYGVAVVLLGVLAWAVVRSKAPAPSMTGDK"
     gene            14722..16911
                     /locus_tag="PL000_12405"
     CDS             14722..16911
                     /locus_tag="PL000_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC89749.1"
                     /translation="MTKESLVLFPGRQEKEFPGDLLRSLTTSVLVLDLLFVLTSFLFF
                     PDLFQRALFFGGGVLLTCILVFLLIYMDKLKLAGVLLVSSLWLMVSYGAYTAGGVDSP
                     IFVGYAAVIMLGALVLGMKTGVAVIFLTIGFSGYLIYAEVNQILPQHISYQPVSRVFI
                     YIFFFFIVLLIQKAVVKTTRMAIGRLNSNESQYRSFLENIASVTYINDLSPDVLTTYV
                     SPQVKNILGYTQEEFLENPSLWMEILHPEDRERVFSESRRTSETGEAFMMEYRVMAKD
                     QRLVWVRDEAALVYDEKGLPQYWLGLWTDITNSKNSESAQLDTLGALLRRTNQLQTAS
                     EVSSAATSILELNELLPKVVELIRSHFDYYYVGIFLVDEKREMAILRAATGDAGRQLL
                     DARHALEIGNTSMIGWCVANNRARIALDVGKDAIRFKNPFLPLSRSELALPLRARGQV
                     IGAMTIQSVVEAAFTDADITALQTMADQVGNAIETARLFDERLRLINELETKNAELER
                     FSYTVSHDLKSPLVTIRGFVGYLWEDAKKGDLSRFEKDMQRIITATEVMQALLNDLLE
                     ISRIGRVTSPLEDIDFGEIVRETLNLILSPSQREMIRIEIQEEFPIVRCDRIRITEVV
                     QNLTANSIKFMGSQPEPKIQFGTAGVDAEKKYPIFFIKDNGMGIEPQHHERIFNLFSR
                     LNPEMEGTGVGLAIVKRVIEVHGGRIWLESQGENKGSTFFFTLPPAE"
     gene            complement(16989..18467)
                     /gene="zwf"
                     /locus_tag="PL000_12410"
     CDS             complement(16989..18467)
                     /gene="zwf"
                     /locus_tag="PL000_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNC89750.1"
                     /translation="MNNLPNNGLLTTIIIFGASGDLTQRKLIPSLFNLFRKRRTPKKF
                     QIIGFGGTAFTDEQFREHLHTGLKEYAGYTYTEEEWNIFAPNLHYLQGKYKEAEDFTK
                     LAEYLSTLEGGASNRLFYMALPPTLFPVIIDNLDASGQLHENGGWRRVVLEKPFGTDL
                     QSAQQLNLQVHKGLNESQIYRIDHYLGKETVQNILFTRFANTIFEPLWNRTYIDHVQI
                     TVAEKVGLEHRAGFYDGIGVLRDMFQNHLLQLLTLVAMEPPASFSASHLRNEKVKVLS
                     AIQPMTPEQVSANTVRAQYKGYRSEDQVNPNSTTPTYAALRLYINNWRWKGVPFYLRS
                     GKNLAEKQSQIIIQFKEPPLAMFPMQTMKPNMLVLYLQPDEGLHVRFEAKAPDTVAET
                     RSVDMEFRYSGAFGQTAIPEAYERLLLDAIQGDASLFTRADEVETAWSLIDPILQTWE
                     THQTPPLAVYRPGSWGPPEGYDLLARDGRRWLNEEASMMLEK"
     gene            complement(18544..19455)
                     /gene="gnd"
                     /locus_tag="PL000_12415"
     CDS             complement(18544..19455)
                     /gene="gnd"
                     /locus_tag="PL000_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNC89751.1"
                     /translation="MELALIGLGKMGLNMATRLVRGGHRVVGYARTKETVETAIKNGA
                     EGAFTLEEAVGKLTSSPKVVWVMVPSGKTTTETIEKVASLLKPGDIIIDGGNSNYKDT
                     IAHAAMLEAKGIDFVDCGTSGGIWGITEGYSLMIGGKEEVTKKLNPIFETLAPGKELG
                     WGRVGPHGAGHYVKMVHNGIEYGMMQAFAEGFGILKGKKEFGLDLSQISHIWQHGSVV
                     RSWLLDLAANALDEDTELKEIKPWVADSGEGRWTVFESIDLDVPAPVITLALQMRFAS
                     RDEENYPARMLAALRNQFGGHAIKKAE"
     gene            complement(19556..20287)
                     /gene="pgl"
                     /locus_tag="PL000_12420"
     CDS             complement(19556..20287)
                     /gene="pgl"
                     /locus_tag="PL000_12420"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNC89752.1"
                     /translation="MESKIHVYKNLQELSTAAADLFAASAAQAISSRGRFLAALSGGG
                     TPKPFHELLATAPYRDQVAWASTHLFWGDERCVAKDDSGNNYFQARQAFIDSVHLPEE
                     NIHRVLSELEPDSAADDYASQLKAFAEAPLAWPRFDLIILGMGDDGHTASLFPGSPVE
                     VNTPTVSVTANYQDRPARRVSLTPLVINAARRVVFLVSGQSKSETLARILHGEPQPDL
                     LPAQRIRPTDGEIIWLVDEAAAGKL"
     gene            20370..21881
                     /locus_tag="PL000_12425"
     CDS             20370..21881
                     /locus_tag="PL000_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNC89753.1"
                     /translation="MKRIIKIILSGLISVTVLTSCIPAAVPTVYAPPTPINDHLPTVF
                     DIEIESPASPARLSDPRPNIIVVLTDDQPYHTVDYMPAVKNQLMKNGVVFENGFVTTP
                     LCCPSRVSILSGQYVHNHEVYTDRMPLGGAPKYDDSECVAVWLQEAGYRTAYFGKYLN
                     GYEDIEPYGVVPPGWDVWKAFLSKNLTADDDVGSTQYFFDFSLSENGTAVEYPRSKDN
                     FSADVITTDALEFIRDERDNPFFMMVGYYNPHSPYIAAPRHKDTFRASADEYWDWVQY
                     RPPNFNEEDIRDKPDYIGDLSPLSPDEVDTAHKQILRSLLSVDDGVASIMSALEKAGL
                     DENTIIVYVSDNGLTLGDHRFGAAKNCPYEACVKIPFIVHAPGRYAPRVDSHLVANID
                     LAPTIAEWAGVTAPAYVDGNSMVPILEDTSADWREDILFEHWPTEEGVGSMIPEFYSI
                     RTADWKYTEYSTGEVELYDLVNDPFELENLAGERDYREIQAELAARLQELKAE"
     assembly_gap    21902..22001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22002..23563)
                     /locus_tag="PL000_12430"
     CDS             complement(<22002..23563)
                     /locus_tag="PL000_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89754.1"
                     /translation="MTSTQKRVFTILIFISIAYFAAFIPPNNTGAKDQMMISLFEPDE
                     FAQYPVIIKMLNPGETLTATLFNFIAYDHYYYGSPFYFSSALLLLPIKLFDSLRNTTQ
                     LNMLLLRQVIGVLPMLGALLLLTHIQTKFSDYAKSIGLFVFLLSVSAVVENNLWWHVD
                     SLAVFFIVLTLFFLDRDDQRFGIHFALAAASTGLATGTKVIGLFFVLTIPTYLLVGIL
                     SKKLTWRDAFLKGLMFVGVMAAAIVISNPFLLLAGQRETMINTISRQASSQTEGWTLA
                     YAKGPASWKHIIEELYGQLLFVALSLVALGVGIWRSPNRIRHLLIATWAFPLGLYILF
                     TTAIKPTHFFLPILLPVYSSLIVFLEFPPFNPEKPRNAASLTWGAVLAAMVIWQFAIY
                     INKDVELYRDVLTREENEGSLVFYDTLERDYLPRITAAEPLTVFRDVRMYFPDNARWN
                     IRTYWNSKYSTIEKIRPDIMILWSQRILDYTQEGALENAVDPDSFQDTYTFYMDVKNE
                     QVRGYHLIYRDA"
     gene            23832..24323
                     /locus_tag="PL000_12435"
     CDS             23832..24323
                     /locus_tag="PL000_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNC89755.1"
                     /translation="MAKIDELVKDFLAQKKIAVVGVSDKRETGCNLGYKNFKGAGYQV
                     FAVNPRISTFEGDPCYADLKSIPEKPDAVFILASPKVTEQMVQQCVDLGIKHVWMHCM
                     MGTKPGLAASMTSVSPSAVEMCKANGITVIPGSCPNQFLNPDGGHKFMRGMWRLFGFM
                     SVN"
     gene            complement(24325..>24683)
                     /locus_tag="PL000_12440"
     CDS             complement(24325..>24683)
                     /locus_tag="PL000_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89756.1"
                     /translation="SEFPIVFEKLKTILSPHAKKLTVKVDTPAAYSLDGPYSEKWKKE
                     LFFGSVQVKKSYVSFYLMPVYMYPDLLKEISPELKKHMQGKSCFNFKTVEKSLFEELS
                     QLIKQGLERFMKEEYS"
BASE COUNT         5611 a         5993 c         6772 g         6207 t
ORIGIN      
        1 cctgtcattg gaaatgttca tgccgcgccg ctggccgacg ctgaagccgc ccgcgccgac
       61 atcatcgcac agatgcaatc ccgcgtgcgc tggaccgaat cggtgcaata catggctgcc
      121 aatggcgtca acacctttgt ggaggtgggc acaggttctg tgcttggtgg ccttgtcaaa
      181 cgcattgccg atggggcggg catttatccg ctgggcaacc cgcaggattt tgcggcacta
      241 gagtaaattg gctcgcgggc gaacttccgc gttattctcg caaaagcggt atatttggta
      301 gagcgacata ccatgtcgct ctacacttat tatgaaaaaa atccttctcc cattcatcct
      361 tattacttta attgttgccg cctgcgagcc gctgccgaca actccgcagt tcgtcacaca
      421 gatccctttt tacgcgagtc agacacccga atttatcatc ccagtgaccg ataccccgat
      481 cgtgtctgag cctttcacgg aaacaccagc gtttatcgtc cctgtcaccg aaactcccac
      541 tcctgaattc atcatccctg tgcccgaaac gccgaccccc gttccgctgg caggctggtg
      601 ggaggtctat tttacagatc cgcttgccat caataacccc aatgcattcg cagggtccat
      661 tgaagaaaag ttgattcaat ggatcaatgc ggctcaggtc agtatccata ttgcgtcgtt
      721 tgaattcaat ctcacgcctg tggccgaagc attgatcgca gcaaagaatc gcggcgtgga
      781 cgtgaaatgg atcacggatg acgaatacgg tctcaagatc gattcagagc ccgagcgtag
      841 acaattctcc cttttaatgg gagcaggcat cgaggtcaag gatgatgcgg ggcgggaagc
      901 cttcatgcac aacaagttct ggatcttcga tcagcaggcc gtgtggacgg gttcgaccaa
      961 tatcacggta aacggcatct tcaaacagaa caacaatgtg atcgtgctgc gttcgccaga
     1021 gatcgcgtac atttacgagc gtgaattcca ggagttgtgg agcgggcaat tcggtccgcg
     1081 tgcgccttcg accatcagca atcaatgggc gattttggat acaaccccag ttcaggtttt
     1141 attctcttcc gaagataacg ccatgaacag cctgctggcc gtggtgaacg atgcgaagtt
     1201 cagcatccgc ttccttgctt ttagttttac tgatgatgcc ctggcacaga ccatgatcga
     1261 gcgcttcaag gctggggtgg atgtgcaggg tgtgtatgaa acgcttgcca gcaccagcat
     1321 ttattcagat ttgaaaacgt tttggtgtgc aggggtgcct gtgaggcagg atggcaatgg
     1381 aagcctgatg cacaacaaag tgatcattgt ggataacagc accgtcatta caggttccct
     1441 gaacttcacg gcgggtgcga atgaaaaaaa tgaagagaac gtcatcatca ttgacaaccc
     1501 cgagatcgcc gcgctgtatt tgcaggaata cgcgaagatc tggagtttgg cccatgatgt
     1561 agaggcaggg acttttacct gccagtaagt ttgcgttaca tttgttacaa ggaaaatggg
     1621 attttcgttc gagagggcat ttaaacccgt atagtataat gacgtcatga acgccatgcc
     1681 tgacaagttg attgtcagtg ccgctttctt cctgcggtta tggaaatgta tctggtcggc
     1741 taattgcctg accagatttt tattttaacg aaatgtcatt gtgggcacgg agcaggcgta
     1801 gctgggaaaa aatccgcgtc acactgacat atcaaggaga cttatgaaac taagcaaact
     1861 tgcactggag atcgctgaat cgccgaccct ggccttgaat gaggaggcgc gtctgttgcg
     1921 tgaacgcggg gaggcggtca tcaatttggg gatcggggag ccaaagaata aaacaccgat
     1981 tgcggccatt ctggcttctg gcgcaaaatt gagcagcgga gaggtgaaat acacaccgcc
     2041 cgatggcttg ccttcgatga agaaggctgt cattcgatac accgaagaga actacaaccg
     2101 actggttgcg cctgagaatg tgatcatcac caatggcgcg aaacaatcgc ttttcaatat
     2161 cttttactcc attttgaacc cacaggatga ggtcatcatc attgcgccgt tttgggtctc
     2221 ttaccctgaa atgatcaaga tgtgcctcgg cgtgcctgtg gtggtcacac ccgaagatgg
     2281 gaccttcact ccgcgttttg aagatatcga acgggccatt acttcatcca cacgggcgat
     2341 cgttgtgaac agccccaaca atccgtcagg cgcgatctac ccgcctgaat tggtcgaaaa
     2401 gatcgtggaa ctgtgtgagc gcaaaggcat ctttatgatc tgcgatgaca tctatcataa
     2461 gctggtcttt ggccgaaatg ttgccgtccc tgcgtactcg ttcaccaaaa aagaaataga
     2521 caattctcac atcatcgttg tcaacggtgt ggcgaaggtc tatggcatga cggggtttcg
     2581 catcggctgg gtgatcgctc cgcgcgatct catcaaagtg atgaccaatg tccttgcaca
     2641 gacgacctct tgcgtgtcgc cgatcgcaca ggccgcggca gaaggtgcgc tgaacggctt
     2701 gcagtctgtg gtggaggcgc tgcgtttgca gattcaaaat aaccgtgagg tggtcttgca
     2761 ggaaatgcgg actttcaatg gggcgcgcct gatcgagccc aaagggacgt tctatgccct
     2821 gctcgattta cgggcattca acacgaattc ggttgagctt tccaagtttc tgctgaaaaa
     2881 ggcgttggtg gtcaccgtgc caggcaggga gtttggcatg gaggggcata tccgcatttc
     2941 gtttgcgggc agcataaaag acatcaccga aggcattgcc cgcatcaaat gggcactcga
     3001 cccaacgtcg ccgaacgaaa tttacattgg cgataagaag atgatacggg attggatgtg
     3061 aaagagtttc aggtgacatg tttcaaggta ctggtcgcaa actttcaccc ttcaacctga
     3121 aaccttcaac ctgactccca aacggagact aacttatgaa taacctgttg aatatcaaaa
     3181 cccccgctga gtccatcgcg cagacgcgta aagcggatta caacttatcg aataacggaa
     3241 ttggaaattt acggcttgcc tattggaacc tgcccaccga ggcgcttgtg gaagaagcga
     3301 ttttccgcaa tgaaggcgca ttggttgcgg gtggtccctt tgtggcaaat acaggaaaac
     3361 acaccgcccg cagtgcgaac gataaattcg tggtgcggca taccgactcc gagaacaaca
     3421 tctggtgggg cgtgtacaac cgtccttttg aagcggacaa gttcgaggtc ctgtatgacc
     3481 gaatgttggg tttcctgcag ggacgtgatg tctttgtgca ggatgtctac ggcggcgcag
     3541 atgaatccta tcgtctgcct gtccgcattg tgaccgagtt ggcatggcac agtcacttcg
     3601 tgcgcaacat gttcatcctg ccgcaatcgc ttgaggaata caaacgcttc gtgcctgagt
     3661 ttacgatcat cgccatgccg tcgttcaagg gcgcgcccgc agtggacagc accaacagcg
     3721 agactttcat ttgcctgtcc tttgaaaaga agctcgccat catcggcaat acggcttacg
     3781 caggcgagat caagaagtcc gtttttacga tcctgaatta tctgcttccg ctcgatggtg
     3841 tgctttcgat gcattgctct gccaatgtga accccgataa cgcggacgat gtcgctttgt
     3901 tttttgggtt gagcggaacg ggcaagacca cgctttcggc cgaccccacc cgccgcctga
     3961 tcggtgacga tgaacatggc tggagcgaca atggcgtctt caactttgaa ggcggatgct
     4021 acgcaaaagt catcgggttg tctgaatctg cggaaccaga gatctacgcc acgacgaaac
     4081 gctttggcac gatccttgag aatgtgcctt ttgatcccgt cacacgctca attgatctcg
     4141 atgatgacac gatcaccgaa aatacgcgtg cttcgtatcc gcttgagttc attgacaatg
     4201 ccgtgcctga aaagaaggcg ggacacccga agaatgtcat cctgctcact tgtgatgcca
     4261 gcggggtgat gccgccgatt gcgcgcctga ccccgaatca ggctttgtat caattcatca
     4321 gcgggtacac ttccaagatc gcagggacag aagtcgggtt ggggaaagag cccgagatca
     4381 cgttcagcgc ctgctttggc ggaccgttca tggtgcatca tccatataaa tatgctgaac
     4441 tgctcaagaa caagatcgag cgttacggtg tgacttgctg gctggtcaac acaggttggg
     4501 tgggcgggcc gtatggtgtg ggcaagcgca tttccatcaa gtacacgcgc gctttgttga
     4561 acgccgcgct caatggaaaa ctggatcagg tcaaattcac gcacgatccg atctttggct
     4621 ttgaggtccc cacccaatgc cccgatgtgc ccgctgaagt tttgaaccct gcttcatcat
     4681 ggcatgacca aaaagaatat gaccgaagat acaaagacct cgcgatgcgc tttaaacaaa
     4741 attttggaaa attcgaaatc ggtaccccgc aggatgtgat cgaggcaggg ccaaaggtat
     4801 agggagggtg gaaagtagcg agtggaaagt gaaacagggc aggcacaaag gcctgctctg
     4861 tttttctacc tgcttgtcgc cgtgtcgagc aggttttcgc cgatgtattt tgcgagaaat
     4921 gtgttgttct cttcgagcgt caccaccacg gtcagcgggg cgccgctcca actgggcagg
     4981 gtgccgccga gaaaccacgt gacaacggta tcgtttgacc cactggcctg accaacgtga
     5041 ctccaaaacg gtttgccttc caggatgaag gaaagcgccg cttcatccgc cgccccggcc
     5101 tgaatcatct caacaggccg actctccgct gtggaaagca ccacccatcc ctgctcgggg
     5161 gtgttcactg cggtggcaat gctcggaact accatcatgc cgtgattgct caacacagat
     5221 gcggccaggc tgatctgtaa cggactgacc cgtaattcat acgcggatgg attttccgta
     5281 tcgtaagcga tgggcatatt gatctgcggc gcctgatata agcccagttt ttgataatac
     5341 gctttcaact cgttgtctgt cggttgcgtt tctccaaaat tcgcgcggat caacggcagc
     5401 atggccgtgc cgatcgggta caacccctgc gtggcacggt tcacgagggg tgccgctgaa
     5461 tttgtcgcct gtgacaaggc ttcgccttct tcatccagct tgttggggtc ataggttggg
     5521 tgcgacgcca tggcaaggat ctcacctgtt tctgcattca tcaaaaggat ggctccgctg
     5581 tggccgccca gcaattggtc tgctttggat tgcagggcaa ggctcaagct gagacgtaca
     5641 tccaacccag gcggaggagt gccgtaaacc agacggtccc accataacaa gctggctggg
     5701 tttccctgta agccgcgcaa ataatcatcg agtgtggctt ccaacccagc ttgaccatag
     5761 atgggatggg tgtacccagt gatgggtgcc aattctgtgt acacatacac acgttgatag
     5821 gttccgcttt cgccttcggt aatgttgatg ggttggttgt cccgatccac caactcgccg
     5881 cgcggcacgt aacggtctgc aatggcgcgg cgcggattgt cggtacggtt gagcaggtca
     5941 ggtgcgcgga tcaccgccca ccatgcggtg gtcaatgcgc tggtggcgag tccgagggca
     6001 agcagacccg caaggaaagt gtatggtttg gggtcttgca gttgggcggg ctcgctgtct
     6061 tcgaggttgc tgatggtgag aagaatgaag agggcgatga aagatgtcag cagcgatgag
     6121 ccgccgtagg aaacgaacgg aagtgtgacc cccgtcagcg gcaccagccg taaattgccg
     6181 ccgatgatga gcaggctttg aatgccgaga taggtcacca cacctgcagc aagataacgg
     6241 cggaatctgt ctgcggcgcg aagtgaggag atcatgcccc gggcgaggag cagccaaatg
     6301 gtgccgagca ggccgatggt cccagccaga cccgtttctt ccgcaatggc cgcatagatg
     6361 aaatcagaga tggcaacagg cacaagcaac gggcttccca atccaggtcc gcgcccgatg
     6421 gtgccgccgt tcgcaactgc cagaagagat tgcacgatct ggtaggactg tcccgatggg
     6481 tcggcccacg gatcaagcca gccttccacg cggatgcgaa tgatgtctat gaaaaaatat
     6541 cctaaaaaga gcgcgagcgc caaaaagaaa accgcgccaa acaaaatgcg ccgcctgcct
     6601 gtggaaagaa aaatgatgac ggtgaaaatg caaatgaaaa tggacgcggt gccgagatcg
     6661 cgttggacga gcaggagcaa aagcgcaacc cctgtaacga ccaacgtggg cacaagcaaa
     6721 gagagcgatg acgagcgaat gttgatgcgg tcggcgaggt aggcggagag ataaatgacc
     6781 agcagtaact tgagcggctc ggagggttga aagtacacgc cgcaacaccc cagccacaac
     6841 cgcggaccga cgccaagcgg atttgtgcca agcaataagg taagcgcggt gatgagcagt
     6901 ccgccgccca gcaatacata tttatatcgc ctgagaaaaa aaagattttg cgggagagac
     6961 attgcaagga tgaagcccgt cacactgagc ccaagccaga tggtttgtct gatcccgaag
     7021 gtttcatcca accgccagat ggtgagcagc ccccatccac tgagtaaagc tgcagcaggc
     7081 agcaggtagg ggtcgcgttc aggcaggtgt ttgatggaag cgcgatgggc ggcggtcatg
     7141 aacacggccc atgcaatgag accgacccaa tgtgagagcc tgtagtctac atcccaggaa
     7201 cgttcgcgta cggctggtgc cagcgtcagg attgtggatt gtatgaggag aaaaattcca
     7261 gcccagcgaa gcaggcgggt ttgaatttgg tcatgcattc attaatggta catcattttt
     7321 cgatgggagt ggaaattggg tttcttcagg aaaacggcgg tttgtgtacg aggcaaggaa
     7381 aaagccctgc atcaagatta ttggtgtcag ggctttttat tcatggctct tgctggtaat
     7441 tttttgagtg cggtattgat ccacttttag aatgattgat aactgcctgc tcaagaggcg
     7501 ttatttcata tatttgccaa ccaacagatc gaggttttgg cgtgtttcag ctgggatcac
     7561 tttcgggttg gtgatcaacg ctgtggcaag tgcttcacca catttgcaat tgcgctgttc
     7621 cttcaatccg cggataaggt tgtgaatggc ttcctgtgct ttttgtgtat tcttgttcag
     7681 ggtctggatg accatttcca cggtgacggg ctcttcgctt tcatgccaga catcatagtc
     7741 ggtgacgtgt gccatggtgg cataacagat ctctgcttcg cgcgcgagga aggcttccgg
     7801 ggaggcggtc atgccgatga tggacatgcc ccaggcacgg aaggcatttg attccgcttt
     7861 tgtggagaag cgcggccctt cgatggtgat taatgttccg ccgcgatggg ttttggcgtt
     7921 ggtggcacgg acggctgctt caaggtcgtt tgagaggtcg gtgcaaaacg ggtctgccac
     7981 accgacgtgc gccacaaatc catttccgaa gaaggagcgg acacggtctt tggtgttgtc
     8041 gtaaatatta tcggggatga caatgtcacc gggcgcgtaa tcttcgcgca gagatccaca
     8101 ggcgctgatg ctgatgatcc gctccgcgcc gagcagtttc aaggcgtaga tgtttgcgcg
     8161 gtacgggact tcggtcggtg tgatgtggtg accgatcccg tgtcttgcaa gaaaggcgac
     8221 tcgcgtccct tccaaagtgc cgatgacgat gggggaactg ggtttcccga agggagtatc
     8281 tatttcatgt tcggttgtgt tttgcaagcc agccatttga tacaagccag agccgccgat
     8341 gagcgcgatg gatgcttttt ccatgttcat tgatgccttt ccactctttg tgttggggat
     8401 acgaaagtgt cggttgaaag attgatgatg aggcgggtgt cgccgcattg aatttcatcg
     8461 ccagaggtca gcactgttgg catggtgacc atgatgttgt tcagggctgt tccattggtg
     8521 gatgcgagat cgtccagcca ccattgcccg tggtgatagg tcaattgcgc gtgacgggtg
     8581 gagatggtgt catcgatcaa agggatgtcg catccgggat cgcgcccaat ggtgatctca
     8641 ggttgggtaa aactttttac gatgggtgcc ccgcgtccat gccgaaccgt gaggctgatt
     8701 ccgggaactc gtctatttgc cagtgagaag ccttgttttt gcacatcacg ccataataca
     8761 aaaagtgccc agcccaggaa tccatacagg gcaagggcca tcataaggcg taaagccaat
     8821 atgatcgggg cgctcatctt ctctttaatt tagctgtgga atgttctttg agcgacgcag
     8881 tggggcgatc tgctgtggcg ctttggaaag gagcggtatc tttcaggtct gggcggggag
     8941 gcgggttgtc ctgaccaaag atcagggcca caccggccaa tgaaatgaca tcaccagggt
     9001 acaacacaac ctgactggcc ctctgtccat tcacaaaggt gccgcctgtt gaattgaggt
     9061 cgaaaaccac gaacctgcct ttgatcgcac gcagttgggc atgattccgt gagacccgcg
     9121 ggtcatcaat gattagttgg ttatccagtc tgcgcccaat attgataaca ggaagtttca
     9181 gcggaaagac cttgactccc tctacaatta gaaaagcatt ttcgggaatc ttgctgtaat
     9241 cttccagatt tccagtgtca agtggggttg cgtttgtgtc tgccataggt tctatccgat
     9301 aagatgccac gacctgggca tcattcaatg gaagtttttc gtctgtcgaa atcgtgatcg
     9361 ttggagatat tgtaacctta aaccccgctt cctgggcgac ggctgagatc gaatgcaata
     9421 ggattgtaag caattgctgg tcctgccatt tggcggcctc gctaggatgc acgatcaggg
     9481 tataaacatt gggcgcaatg accgagccat cggtggcagt ggtggtatta agttgaatgg
     9541 ccgccgccag tttttgcacg atcacatctt caaccttgcg gctggggaga attttcaata
     9601 aatccacttc gatcagagct tgcagacggg cttcgagttc tttcagattc attttttcct
     9661 gttaatcttt attattaaag gtcctggccg cagttctggc actttttgtc atcaggccgg
     9721 gctacataac cgcaatgccg gcaggtacgc ggttgcactt tgccaagcgg agccccgcag
     9781 gcaacacacg aagggtcacc aacaacattt gctgttccac agtattcaca ggtcaatctc
     9841 ttgaggcggt ccgataactc agtggatgcc ccggcggcgc gcgcagtctg gttgatcacc
     9901 tgccagatct tttcactcat ctgaaggttt tcgatgtcct gggcaatatc atccagccga
     9961 ccaagcaagc tgaagggatt cctcagagca gcgagcgcac tcattcccaa actggcagcc
    10021 acgccaagcc aggcctgctg gcccaattcc accatgatgc catcttcatg cttttggatc
    10081 gtcacagtca tggccgtttg cccgccagac attgccccag ggtgggttcc cacctgaacg
    10141 atcatcttgt cactttgtcc aaaggactgt gcctgtaaat ttccctgatt gaattcaccc
    10201 agcagagctt gtgcaacatc cactggtttg agatttccgt gaaaaatttt tcgttccatg
    10261 aagtagatta taatcgcctt tgcgattctc cttaagagtt acttacgaaa tgaagcgaat
    10321 ctggtttcac cccaccctcc tgatcatttt cacaaccttg ttttccctct ggacaatttt
    10381 tttcgcgtct tcctcgaccg ttgtcctggc ccagcaaccg acagagactc ctgcttcaac
    10441 aggaatgttc atcacagtca tcaccgatga gccgcagatc aatgtgcgca tgggaccgag
    10501 ctcttccatt taccccattg tcggcaccct gttgagaggg tcaaccgccc cagcgcttgg
    10561 acggtctgca ggcggtgact gggtgcagat tgaattcatc ggcgctcctt acaataaagg
    10621 ttgggtgtat tcgccgcttg tgcaggtctc cccgggaacg cttctagtag tggaagcccc
    10681 gcccacgcct gtgccgccgc caacttcaac cgttgaccca actctggcgg cgcaatttag
    10741 cattgtccca acgtctacac gtctgcccac cttcacgcct ccgccctcat tggtggttcc
    10801 ttcatacaca caagctcccc cggtcatttc cagccagact ggctctctgg caattatcat
    10861 tattgcgctt gctgttttgg gtatgatcgg tttcctgctt tccctatttt tgcgccgata
    10921 ataatatgat ttttgcaaga cgaattcgcc ttacggcatt cattttggtc atgtttttgg
    10981 gcatggtgtt tgcattccat tcccctgtgc cgaccacggt ccatgcccag cagccaacgg
    11041 gttcaattcc aactgtaact ggaacacccg gtggcgccac tgtagccttg tatttggatg
    11101 aaggacaccc acaggagtat gtttactcgg gtcccagtat ttatctttac ccggccattg
    11161 gggttttgct ggcgggtcag gaagttcctg cctacgggta ctctgaagat aaaaattgga
    11221 ttcaaattta ttatcccggt gtgcctaact cagtagcctg ggtatatgca ccttatgtaa
    11281 aagttgtaaa gttcggtgaa ttgcctttgg tggcagctcc gccaacccca acccctgcat
    11341 ctacacccgt tgtaaatatc aacccaacct tggttgcggc gttcagtacc cccgttttgc
    11401 cgacaaggct gcccactttt actccacccg tccctttggt ggttgcaact ttcgtagatg
    11461 aagcagtcaa cacgaatcgt gtgccggtgg gcctcttgat tttcggtttc ggtttcgtcg
    11521 gcgcattggg aacgatcatt tctttcctgc gcggaagatg atccgtgcca ttaaataaaa
    11581 atgaccggtg ttacccggtc attttttgat tcaaaaaagc ctagttgctg caaccgcagg
    11641 caccgccgcc gccgcaggca caggatgaac caccactgtt cgtgctgcag gcgcaaccgc
    11701 cttcaccgtg ggagtgtcct tttgcgttgg ggctgctgac tgcaaatccc tgtccgtggt
    11761 gtgggtcgtt tacgaaatcg accgtcgcat cgcggagata gtcaatggaa acttcatcaa
    11821 ccacaacctt gacgccgttg gcttcgaatg tggtatccac actgcggaag ttgttgtcaa
    11881 gcgccatgcc aaaatttacg ccgcagcaac caccgcctgc aacatacacg cgcagggcgt
    11941 aaccttcaag gttgcgctgt tgaagaatat ctttgacagc ctgactggcg gcaggagtta
    12001 atgtgaacag ttgggtttca atttcttgaa gcatgttttc tctcctgatt ttgagtttac
    12061 ttctaatttc gctagatatt atcagatttg gggtggactg tcaacgtgtg tcaataaaac
    12121 ttgatttgac atccacattt cttgacacat tcatcttgca actgtaaaat ccctctgctt
    12181 aataggcgca agccaacatt ttttattccc cccggaggat ataccgcaca tgtattatat
    12241 gcaaggactt acctcattgg aggcgatcgc gatctgggtc gtatttggca ttgccattat
    12301 cggcttgttg tacgcgctct tcctccgctc ccaaatcctg cgtgaagata aaggcactca
    12361 aaagatgcag gaagtctggg gtgcgatcaa agatggtgca gatgcctacc tgcgcaagca
    12421 gttccgctcc attctgcctc tgatcggcat tttgacgatc gctttgttcc tgtccgttta
    12481 tgtcgttccg ccatctcctg aagcccgtga acgcttctcc accatgaccg aaagccaggt
    12541 ccgtttctgg atcggtctgg cgcgcgcact cgcctttatc atgggcgctg ggttctcact
    12601 tactgtgggt cagctcggta tgcgtatggc tgtggaaggc aatgtgcgtg tggctgccgc
    12661 ctcaaagaag tcttttggtg atgcattgcg cgttgcctat cgtgccggca caatcaccgg
    12721 catgctcaca gacggtttgg gtctgcttgg cggtaccatc atcttcatca tccttggtat
    12781 tgcagctcct gacgccctgc ttggcttcgg tttcggcggt acactcctcg cgttgttcat
    12841 gcgcgtgggt ggtggtattt tcacgaaggc tgcagatgtc ggcgctgacc ttgttggtaa
    12901 ggtggaagca ggcatccctg aggacgatcc gcgcaatcct gctgtgattg cagacctcgt
    12961 aggcgataac gtcggtgact gtgctggtat ggccgctgac attttcgaat cctatgaagt
    13021 gacgatcgtg tcgggcctga ttcttggtct ggccctctgg cacacaaccg gccgccttga
    13081 gtggatcatc ttccccctca tggttcgtgg tattggcgtg ttgtcttcca ttatcggcac
    13141 ctactttgtg aagggtggcg agactggcaa gagcgaagac gcgatgaagg ccatcttcag
    13201 cggctttatt acttctgctg ggatctccgc cgcattgttc ttcattgctg gtttctttta
    13261 catgaaagac accatggttg attacggtgg ctggtggcgt atgccgatgg ctgtgggtgt
    13321 gggtgtgttg cttgcgatct tgatcgatcg cctgaccgaa tactttactg gtacacatgc
    13381 ctctcctgtg aatgacatca agaaggccgc agataccggc cctgcaacct tgatcttgaa
    13441 tggtatttct gttggtttcg agtcgtccgt ttggtctgtt ctggtcattg cgttgaccat
    13501 catcgcttcc atctttattt tcggaacgat ccccggtgta acgggtgcgg aacgcgcgac
    13561 cttcattctt tacggtgtgg ccatgaccgg tatcggtatg ttgaccttga ccggcaacaa
    13621 cgtggcaatg gactcctttg gcccgatcgc agacaatgcg aacggcattg gtgaaatgtc
    13681 atggtctggc atggaagata aggaaaccaa aaaggcccag cagatcatgg ctgacctgga
    13741 tgcggtgggt aacaccacca aggccattac caagggtgtg gccattggct cagcggtgat
    13801 tgctgccgtt tccttgttcg gttccttcct tgtggacgtt tcccgcgctc aagaaagcct
    13861 tggcattcct gttgaccagc agattcaggc aattggtatc cgtgtggata tcccgcagat
    13921 ttttgtgggt atgctcatcg gtggcgcttt gccctggctg ttctcctctt tcgccattca
    13981 ggctgtgacc cgcgctgctt ctctcattgt gcttgaagtc cgccgccagt ttaaactggg
    14041 tgtgttggat ggaaaggtca agcctgatta cgctcaggcc gtcaacatct ccaccgctgc
    14101 tgcgcagaag gaattggtat ccctcgcatt gctcggtatt gtgaccccga tcgttgtggg
    14161 cttgttcctg caagtggaag ccctcggcgg ctttttagcg ggcatcatcg tttctggcca
    14221 gttgctggct gtgttcctca acaactctgg cggcgcctgg gataatgcca agaaactgat
    14281 cgaagatgag cccaaggatc ctgccaaaaa ccttggtaag ggttctgaac gccacaaggc
    14341 tggtgtggtg ggtgacaccg tcggtgatcc gttcaaggat acggctggcc ctgcactcaa
    14401 cccgatgatc aaggtggtga atctggtcgc cgtgatcatt gccccgatca ttgttcaata
    14461 ctccacagcg accggcggcg ttttgttcgt tgtttatggc gtggctgttg tattgcttgg
    14521 cgttctggct tgggcggttg ttcgctccaa ggcgcctgct ccatccatga ctggcgacaa
    14581 atagataaac cgtaatatat gaaaaaagaa gactggcgat caaaatcgcc agtcttcttt
    14641 tttggacgga acctgtatgg tttttaaaaa atagggaaat gggaaactaa gataaaattg
    14701 ggcagagctt taagataaaa catgacaaaa gagtccttgg tgttattccc cggtagacaa
    14761 gaaaaggaat ttccaggtga cttgttgcgt tcccttacaa ccagcgttct tgttttggac
    14821 ctgctgtttg ttctgacttc gtttttattc tttcccgacc tgtttcaacg agctttgttt
    14881 tttggcgggg gtgtcttact gacttgtatt ctggtctttt tgttaattta tatggataag
    14941 ctgaagctgg ccggagtgct tttggtgtct tctctttggc tgatggtctc ctatggggcg
    15001 tataccgcag gtggggtgga ttcaccaatt tttgtcggtt atgcagcggt gattatgctc
    15061 ggtgccctcg tattgggaat gaaaacaggg gtagcggtca tttttcttac gattggcttt
    15121 agcgggtacc tgatctatgc agaagtaaac cagattctgc ctcaacatat ttcttatcaa
    15181 cctgtttcca gggtttttat ttatattttc ttctttttca tcgttcttct tattcaaaaa
    15241 gccgtcgtga aaacaactcg catggccatt gggcggctga actcgaacga gagtcagtat
    15301 cgatcttttt tggaaaatat tgcctcggtt acctatatca atgatttatc gccagatgtg
    15361 cttactactt atgtcagtcc gcaggtgaaa aatatattgg gatataccca ggaggagttt
    15421 ttggaaaatc cctccctgtg gatggagatt ttgcacccag aagacaggga gcgggttttt
    15481 tccgaaagcc gacggacctc tgagaccggt gaagccttca tgatggaata ccgtgtcatg
    15541 gcgaaggatc aaaggcttgt atgggtgcgg gatgaagccg ccctggtcta tgatgaaaaa
    15601 ggactaccgc aatattggct ggggctgtgg accgatataa ccaacagtaa gaattctgaa
    15661 agcgcacagt tggataccct gggcgcactt ctgcggcgca ccaatcaact tcaaaccgcc
    15721 agtgaagttt cgagtgcagc aacgtccatt ttggagttga acgaattgct tcccaaagtg
    15781 gtggagttga tccgttcaca ttttgattac tattacgtcg gcatttttct ggtggatgaa
    15841 aagcgcgaga tggcgatact gcgagcggcc acgggtgacg ctggcaggca actgctggat
    15901 gccagacacg cccttgagat cggaaatacc tccatgatcg gctggtgtgt ggcaaacaat
    15961 cgggcgcgta ttgcgttgga tgtggggaag gatgccattc gtttcaagaa tccttttttg
    16021 ccgctttccc gctcagaact ggcacttcca ttgcgtgccc gtggacaggt aataggggcg
    16081 atgacaattc aatcagtggt ggaggcggcc ttcactgacg ctgatattac cgccctgcag
    16141 accatggcgg accaggttgg aaatgccatt gagaccgcgc gtctttttga tgaacgtttg
    16201 cgattgatca acgaattgga aaccaagaat gcggaattgg agcgtttttc ctataccgtg
    16261 tcacatgatc tgaaatcgcc gcttgtgacc attcgcggat ttgtcggata tttgtgggaa
    16321 gacgccaaaa aaggggattt atcgcgcttt gaaaaagata tgcagcggat tatcactgcc
    16381 accgaagtga tgcaggccct gttgaacgac ctgctcgaga tttcgcggat cgggcgtgtg
    16441 accagcccgc tggaggatat tgattttgga gagatcgtca gggaaacctt gaatttgatt
    16501 ctcagcccgt ctcaacggga gatgatccga attgaaatac aggaagaatt cccgatcgtt
    16561 cgctgtgacc gcatccgcat taccgaagtc gtgcaaaacc tgaccgccaa ttccatcaaa
    16621 ttcatgggca gccagcctga gccgaaaatc cagttcggca cagcaggtgt ggatgctgaa
    16681 aagaaatatc ccattttctt catcaaggat aacgggatgg gcattgagcc ccagcatcac
    16741 gaacggattt tcaacttgtt cagccgcttg aaccccgaaa tggagggaac cggggttggc
    16801 cttgcaatcg taaaacgcgt cattgaagtc cacggcgggc ggatatggtt ggagtcgcag
    16861 ggggagaaca aaggcagcac tttctttttc actctgccgc ctgcggaata aataatcgtc
    16921 caaagcgttc cagaaattta atggaaataa aaagtcgaga tgccattgca cgcacctcga
    16981 ctttttattt atttttccaa catcatgctg gcttcttcat tgagccagcg ccgcccatca
    17041 cgggcaagca ggtcgtatcc ttcggggggg ccccagctac cgggtctgta aacagccaac
    17101 ggcggagtct gatgcgtttc ccatgtttgc agaatcgggt cgatcaacga ccatgccgtt
    17161 tccacttcat ctgcgcgggt gaagagcgaa gcatcgcctt gaatggcatc cagcaacagc
    17221 ctttcgtagg cttctggaat cgccgtttgc ccgaacgccc ccgagtaacg aaattccata
    17281 tctacggacc gtgtctctgc aaccgtgtct ggcgcctttg cctcaaaacg gacatgcaac
    17341 ccttcatcgg gctgcaaata aagcacaagc atgttcggct tcatcgtctg catggggaac
    17401 atcgccagcg gcggttcctt gaattgaata atgatctgcg attgtttttc ggcgaggttt
    17461 tttccagacc gcaaatagaa cggcacgcct ttccagcgcc agttgttgat atagagacgg
    17521 agcgccgcat atgtgggcgt ggtggaattc gggttgacct ggtcttcgct gcgatagcct
    17581 ttatattgtg cgcgcaccgt atttgccgaa acctgctcgg gcgtcatggg ctggatcgcg
    17641 ctgagaactt ttactttctc attgcgcaag tgacttgcgc tgaacgacgc gggcggttcc
    17701 atggcaacca gcgtgagcaa ttgcaagaga tgattttgga acatatcccg caacacgccg
    17761 atgccatcgt aaaaccctgc acgatgttcg agaccgacct tttcagcgac ggtgatctgc
    17821 acatggtcaa tgtatgttcg attccagagt ggttcaaaga tcgtgttggc aaagcgggta
    17881 aacaaaatgt tctgcacggt ctctttacca aggtaatgat cgatgcggta gatttgcgat
    17941 tcgttcaagc ctttatgaac ttgcagattc aattgctgtg ccgactgcag atcggtgcca
    18001 aacggctttt ccaataccac ccgccgccag ccgccatttt catgcagttg acctgacgca
    18061 tcgagattgt caatgatgac cgggaacagc gtaggcggga gcgccatgta aaacaggcga
    18121 ttggacgcgc cgccctcaag tgtgctcagg tattcggcca gcttggtgaa atcttcggct
    18181 tctttgtatt ttccctgcag gtagtgcagg ttgggcgcga agatgttcca ttcttcctcg
    18241 gtgtaggtgt agccagcata ctctttcagc ccggtatgca aatgctcgcg gaattgctca
    18301 tcggtaaagg cggttccacc gaagccaatg atctgaaact ttttcggtgt gcgccgcttg
    18361 cgaaacaaat taaagagcga tggaatcagt ttgcgctggg tcaggtcacc cgaggcaccg
    18421 aagatgatga tcgttgttaa taagccatta ttgggtaggt tattcataaa taaatgaccg
    18481 tggacagtgg acgatggacc gcgaaaagtt cccacggcct acggtccatc gtctatctag
    18541 cgtctattca gctttcttga tggcgtgtcc gccgaattgg ttgcgaagcg cagccagcat
    18601 acgggcgggg tagttttctt catcacggct ggcaaagcgc atttgcagtg caagggtgat
    18661 gacgggggca ggcacatcca ggtcaatgga ttcgaagacc gtccagcggc cttcgccgga
    18721 atcagccacc cagggtttga tttccttcaa ttcggtatct tcatcgaggg catttgccgc
    18781 aaggtcaagc agccacgagc ggacaacgct gccatgctgc caaatgtgcg agatctgtga
    18841 aagatcgagg ccgaattcct tcttgccctt caaaattcca aaaccttctg caaaggcctg
    18901 catcatgccg tattcgatgc cgttgtgaac cattttcaca taatgacctg cgccatgcgg
    18961 accgacacgt ccccagccca attcttttcc gggggccagc gtttcgaaga tcgggttcaa
    19021 ttttttggtc acttcttcct tgccgccgat catcaggctg tagccttcgg tgatgcccca
    19081 aatgccgccg ctggttccgc aatccacaaa gtcgatgcct ttggcttcaa gcatggctgc
    19141 atgagcgatg gtgtctttgt agttcgagtt gccgccatca atgatgatat cgccaggctt
    19201 taataaggaa gcaacctttt caatggtctc tgtggtggtt ttgcctgagg gcaccatcac
    19261 ccagacaact ttcggagagg aggtgagctt gcccacagcc tcttccaggg tgaatgcgcc
    19321 ttcagcgcca ttcttgatgg ctgtttcaac ggtctctttg gtgcgggcgt acccaaccac
    19381 gcgatgacct ccgcgtacga gccgtgttgc catgttcaat cccatcttac ccaagccgat
    19441 cagtgcgagt tccatattct ttgctcctaa attttatttc cactcgattt caagggcagc
    19501 ggactaaagt ctgcggtcct gtgttttgag aggatgattt tcacccgaga attttttata
    19561 gtttgccagc cgctgcttca tccaccagcc agatgatttc tccatcagtg gggcggatgc
    19621 gttgggcggg taacaggtct ggctgaggtt ccccatgtaa gatgcgggca agggtctcgg
    19681 acttactttg tccactgacc agaaagacca cacgccgagc cgcattgatg accaacggtg
    19741 tcagcgagac acgcctcgca ggacggtctt gataattggc tgtgacagaa accgttggcg
    19801 tgttgacctc caccggggag ccagggaata acgaggccgt atgcccgtca tcgcccatgc
    19861 cgaggatgat gagatcaaag cggggccagg cgagaggcgc ctcggcaaat gctttcaact
    19921 ggctggcata atcatcggcg gcagaatcag gttccagttc agagaggacg cggtgaatat
    19981 tttcctcagg caggtgaacg ctgtcaataa atgcctgtct tgcctgaaaa taattatttc
    20041 ccgaatcatc ttttgccacg cagcgctcat caccccaaaa gaggtgagtg ctggcccagg
    20101 caacttgatc gcggtaaggt gcagtggcaa gcaattcatg gaaaggtttg ggcgtgcctc
    20161 cgcctgaaag tgccgcgagg aaacgcccgc gtgaactgat cgcctgcgcc gcagacgcgg
    20221 cgaataggtc tgctgctgcg gtgcttaatt cttgaagatt tttatagacg tgaattttgg
    20281 attccatcgc ccgagtttat cacgatgcgg gttgaattta taagttcttg ttacaattca
    20341 ctttgcaacc caacctgcct ggagaataga tgaagcgaat tataaaaatt atcttgagcg
    20401 gcctgatcag tgtcaccgtg ttgacctcct gtatccctgc tgcggtgccg accgtgtatg
    20461 ctccgcccac gccgatcaac gatcaccttc cgacggtttt tgatattgag atcgaatcac
    20521 ccgcctcgcc tgcgcgtttg agcgatccgc gcccgaacat cattgtggtg cttaccgatg
    20581 accagccata tcacacggtg gattacatgc ccgctgtgaa aaatcaattg atgaaaaacg
    20641 gggtcgtatt cgaaaatgga tttgtcacca caccgttgtg ctgcccaagc cgtgtgagta
    20701 ttttatcggg gcagtatgtt cacaatcacg aagtctatac cgaccgtatg ccgttgggcg
    20761 gtgcgcccaa gtatgatgat tcagaatgtg tggcggtgtg gctgcaagag gctgggtatc
    20821 gcacggcata ttttggaaaa tacctgaacg gttacgaaga tatcgagccg tatggagttg
    20881 ttccgccagg gtgggatgtg tggaaggctt ttctcagcaa gaacctcact gccgatgatg
    20941 atgtgggcag cacacaatat ttttttgatt tcagcctttc ggaaaatggg accgctgtgg
    21001 agtacccgcg ctcgaaagat aatttcagcg ccgatgtgat tacgaccgat gccctggaat
    21061 tcattcgcga cgaacgcgac aatccgtttt ttatgatggt cggttactac aacccgcatt
    21121 cgccatacat cgctgcgccg cggcacaagg acaccttccg cgccagtgca gatgaatatt
    21181 gggattgggt ccaatatcgt ccgccgaatt ttaatgaaga ggacatccgc gacaagcctg
    21241 attacattgg ggatctttcg cctctttctc ccgatgaagt tgatactgca cacaagcaga
    21301 ttctacgctc gctgctctca gtagatgacg gcgtcgcttc cattatgtct gcacttgaga
    21361 aggcaggcct ggatgaaaat accatcattg tttatgtttc tgataacggt ttgacattgg
    21421 gtgatcatcg ttttggtgcg gcgaaaaatt gtccgtatga agcctgtgtc aagatcccgt
    21481 tcatcgtcca tgctccgggc aggtacgccc cacgggttga ttcgcatctc gttgcgaaca
    21541 ttgatcttgc ccccacgatc gccgaatggg ctggtgtcac tgcgcctgcg tatgtggatg
    21601 gaaacagtat ggtgccgatc cttgaagaca cgtctgcaga ttggcgggag gatattttat
    21661 ttgagcattg gcctacggaa gaaggcgtcg gctcgatgat tcccgagttt tattcgatcc
    21721 gaactgctga ttggaaatac accgaataca gcacaggtga agtggaattg tatgacctgg
    21781 tcaatgaccc gtttgagttg gaaaatctgg caggggagcg ggattacagg gaaatccagg
    21841 ctgagctggc ggcgagattg caggaattga aggccgagtg attttgtgcc gcaacattta
    21901 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcggcgtcc cgatagatca
    22021 ggtgataacc gcggacctgt tcgtttttga catccatgta aaaggtgtag gtgtcttgaa
    22081 atgaatcagg gtcaaccgcg ttttcgagcg cgccttcctg agtgtaatca aggatgcgtt
    22141 gtgaccagag gatcatgata tcggggcgga tcttttcgat ggtgctgtat ttgctgttcc
    22201 agtaggtgcg gatgttccag cgggcattat cggggaagta catgcgcacg tcgcggaaga
    22261 cggtcaacgg ctcggcggcg gtgatgcgtg gaagatagtc acgttcgagg gtgtcgtaaa
    22321 aaacgagaga gccttcattt tcttcgcggg tgagcacatc gcggtacagt tccacatcct
    22381 tgttgatgta gatcgcaaat tgccagatga ccattgcggc aaggactgcg ccccaggtta
    22441 gcgatgcggc attgcgaggt ttttcagggt tgaacggcgg gaattcaagg aaaacgatca
    22501 ggctgctgta cactgggagc agaatgggca ggaaaaaatg cgtcggtttg atggcggtgg
    22561 tgaagagaat gtacaagcca agcggaaatg cccaggttgc gatgagcagg tgacgaatcc
    22621 gattcgggct gcgccagatg ccgaccccaa gcgccacgag cgacaatgcg acgaaaagca
    22681 attggccgta gagttcttcg atgatgtgtt tccatgaggc ggggcctttg gcataagcca
    22741 gagtccagcc ttcggtttgg gaggaggcct gacgtgagat ggtgttgatc atcgtctcgc
    22801 gttgacctgc taataaaagg aagggattgg agatgacgat ggctgccgcc atgacgccga
    22861 cgaacataag gcctttgaga aaagcgtccc gccaggtcaa ttttttggag aggatgccga
    22921 cgagcaggta ggtgggaatg gtcaaaacga agaaaaggcc gatcaccttg gtgccagttg
    22981 ccagtccggt ggaagcggcg gcgagggcaa agtgaatgcc gaagcgttgg tcatctctat
    23041 caaggaagaa caaagtcagc acgatgaaga agaccgcaag actatccaca tgccaccata
    23101 ggttgttttc aaccacggcg gaaaccgaga gaaggaatac aaaaagaccg atagacttcg
    23161 cgtagtcact gaatttggtt tggatgtggg tgagcagaag aagcgcgcca agcatgggca
    23221 aaacgccgat gacctgccgt agcaggagca tgttgagttg ggtggtgttg cgcaggctgt
    23281 cgaagagttt gatgggcagg agcagcaggg cgctggagaa gtagaaaggt gagccgtagt
    23341 agtagtggtc gtaggcgatg aagttgaaga gggtggcggt gagggtttcg ccggggttga
    23401 gcattttgat gatgacgggg tattgagcga attcgtcagg ctcgaagagc gagatcatca
    23461 tctggtcttt ggcgccggtg ttgttggggg ggataaaggc ggcgaaatat gcgatgctga
    23521 taaaaatgag gatggtgaag acgcgttttt gagtggaagt catgcgggca atgataccat
    23581 ttttggattt gacctcttgc ataagtaaag tgttgtagac cacaaagcca ccaaggcacc
    23641 aagtcactaa gttttttttc tttgagtcta tgtgggtcgg attgccaatc cgacctacgg
    23701 ttgacgggcg ttttcctgat aaaaactttt tgctttggcg tttggaagga gataaaggtg
    23761 atttttgtca cctgcatttt gcggaacgga atgcctataa tttcgtaagt tccatcaagg
    23821 agaattttga aatggcaaaa atagacgaac ttgtaaaaga tttcctcgcg cagaaaaaga
    23881 tcgcggtggt gggtgtttca gacaagcgcg agacgggctg taatttaggt tataagaact
    23941 tcaagggtgc ggggtatcag gtgttcgcgg tcaacccgcg aatctccact tttgagggcg
    24001 atccgtgtta tgcggatctg aaatccatcc ctgagaagcc cgatgcggta tttatccttg
    24061 ccagcccgaa ggtgaccgag cagatggtgc agcaatgtgt ggacttagga atcaagcacg
    24121 tgtggatgca ctgcatgatg ggcactaagc cagggttggc ggcgagcatg accagcgttt
    24181 caccatctgc ggtggagatg tgcaaggcga acgggatcac ggtgattccg gggtcgtgcc
    24241 cgaatcagtt tttgaatccc gatggcgggc ataagttcat gcgcgggatg tggaggttgt
    24301 ttgggtttat gagcgtgaat tgaattaaga atattcttcc ttcataaatc gttcgagtcc
    24361 ctgtttgatg agttgactta actcttcgaa cagggatttt tcaacggttt tgaagttgaa
    24421 gcaggatttt ccctgcatgt gttttttgag ttcaggggag atttctttga gcaggtcggg
    24481 gtacatgtaa acaggcatga ggtaaaacga gacgtaactc tttttgacct gcaccgagcc
    24541 gaagaagagt tcttttttcc atttttcgct gtagggaccg tcgagcgagt atgcggcggg
    24601 ggtatccact ttgacggtta gtttttttgc gtgagggctg aggatggttt ttagtttttc
    24661 gaagacgatg gggaattcgg atg
//
LOCUS       DAOQLC010000065        24607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_681_length_24607_cov_27.349137, whole genome shotgun sequence.
ACCESSION   DAOQLC010000065 DAOQLC010000000
VERSION     DAOQLC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_681_length_24607_cov_27.349137"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1131
                     /locus_tag="PL000_12445"
     CDS             100..1131
                     /locus_tag="PL000_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89757.1"
                     /translation="MTKTDRKPLLEVRNLKTYFYTEDGVVSAVDGVSFEVYPGEVLGI
                     VGESGCGKSVTSLSIMRLIAPPGKITEGEILLDGKNLLNLSEDEMTKMRGNKISMIFQ
                     QPQTALNPVFKVDNQIAEVLNIHKAVGKEAGRQRAIELLKMVGIPDAERRADAYPHEL
                     SGGMAQRVMIAMALACTPELLIADEPTTALDVTIQAQILDLMRDLRTKMGTSVILITH
                     DLGVVAEMAERVAVMYAGEVVEQAEVNSLFEQPLHPYTQGLIGSIPVLGQIKERLDVI
                     PGSVPNLVNLPPGCRFAPRCTAREKFGLKICTEVKPELTDAAPGHKVRCWLYQNAKDH
                     QAPLKAKQG"
     gene            1197..2189
                     /locus_tag="PL000_12450"
     CDS             1197..2189
                     /locus_tag="PL000_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89758.1"
                     /translation="MSQTTEMQKTEDLVQVEHLVKYFPVRAGLMQRVVNWVKAVDDVS
                     FTVKKGETLGMVGESGCGKTTIGRSMLRLVEPTSGSVQYDGRDVLKLRGSELKDVRRH
                     MQIIFQDPYASLDPRVPIGESVMEGLHIHKIGSPQERYDLMIETLKKVGLEDYHARRY
                     PHEFSGGQRQRIGIARALALRPNFIICDEPVSALDVSIQSQVLNILKDLQKEFGLTYL
                     FIAHNLSVVEHISDRVAVMYLGKMVELTSRDDLFKNPLHPYTKALMSAIPIPDPKLKR
                     QREVLKGDVPSPLNPPKGCRFHPRCPIADKICSEQEPEFREAAAGHFVACWMVK"
     gene            2196..3296
                     /locus_tag="PL000_12455"
     CDS             2196..3296
                     /locus_tag="PL000_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89759.1"
                     /translation="MDRNILLVDDQRDILRLLRSTLETLKKTEIKIFEAPSGEEALIE
                     SSRHKVDLLVTDYKLPGMSGLELMHKVRVRHPEAKVILITGLTDRKVREEMLNAGALA
                     VFDKPIPLADFLDVVERGLGFVQTIFPPERPSDEKTERRHARVSDLLANFRQDINAEA
                     VFLLNERGLVQARTGKLRDESMEVSLFSTLMAIHNASLKVAKYNHQEKLDSYHVFSSG
                     DNDLLFIPVAPMYALLVAGNGLVREDRILESVNALLALRKEVERSLRSLGVTGELQSI
                     SPKTVPLATAAAPTIPIPASIKPMEKPAEAAPSPELEALLKNAAATKDQTANMDDFWN
                     QAAEKHGNKPASKEVISLEDARKLGLLPGDGK"
     gene            3325..3398
                     /locus_tag="PL000_12460"
     tRNA            3325..3398
                     /locus_tag="PL000_12460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3357..3359,aa:Gln,seq:ttg)
     gene            3539..4900
                     /gene="glmU"
                     /locus_tag="PL000_12465"
     CDS             3539..4900
                     /gene="glmU"
                     /locus_tag="PL000_12465"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC89760.1"
                     /translation="MKVTAVLLAAGQGTRMKSSLPKVLHPIAGRPLIWHAMQAISQST
                     TEKPIVVVGHGAEEVTKYLGDSAQTVLQEPQLGTGHAVMQAEDLLRGKTDLVVVCYSD
                     MPLLRGETLQALVETQKKNKGPISMLTVHADDPRGFGRIIRKTDGTVDAIVEEYVATP
                     EQLQVKELNVGGYCFDANWLWDALHRIPKNPKKGEYYLTDTVELASQAGLPVQATVMD
                     DVEETIGINTRIHLADAEAAMRRRINQQHMLNGVTMIDPASTYIEAGVSIGRDTVIMP
                     NTYLHGRTEIGEGNVIGPNTIIHDSKIGNHCKVLASVMEGALLDDHVDMGPFARLRKG
                     AHLGNHVHMGNFGEVKDSYLADGVKMGHFSYIGNAQIGSNTNIGAGTITCNYDGEKKH
                     PTEIGEDVFIGSDTMLVAPLKIGAGARTGAGAVVTKNVSEDTLVVGMPARAIKKLERK
                     TKK"
     gene            4928..5332
                     /gene="cdd"
                     /locus_tag="PL000_12470"
     CDS             4928..5332
                     /gene="cdd"
                     /locus_tag="PL000_12470"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNC89761.1"
                     /translation="MDLTNDERQTLIKQAVEIRKRAYVPYSNYPVGAAVRTKSGKVFT
                     GVNIENAAYPQTMCAERVAIFKAVSEGESEFDVITVVTNNGGSPCGGCRQVMAEFGLD
                     TVVLIADGDGNLHKELTVADLLPEAFTPKHLQ"
     gene            5387..6145
                     /gene="recO"
                     /locus_tag="PL000_12475"
     CDS             5387..6145
                     /gene="recO"
                     /locus_tag="PL000_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNC89762.1"
                     /translation="MTEFRSFRASAVVVRHTDWGEADRLLTLYTREQGMVKALAKGAR
                     KINSRKAGHLQPFTHVTVQLAKGRDLLIVTQAETVDAFLPLHDNLVKTGYAAYIVELL
                     YRFSYEEEGGNPSLFKLLVDTLGRIEKEDEAWLPVRYYEMRLLDIVGFRPQLFECANC
                     GREILPEDQYFSFVTGGVICPQCGLGLPNLAKISMEALKYFRHFQRSSYRDASRAHPS
                     FEVQKEAEALMQGYFTYLLERELNTPGFIKRVKS"
     assembly_gap    6211..6310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6311..7075)
                     /locus_tag="PL000_12480"
     CDS             complement(<6311..7075)
                     /locus_tag="PL000_12480"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC89763.1"
                     /translation="MGTADLHIHSIYSHDATTTVRAILKQAADVGLDVIAVTDHDEIR
                     GSLEACELAPKYGVEVIPGAEVTTKDGHLVALFVETLPPAGLPLVDTLIHIGKQGGIA
                     IAPHPFNNLPGSLSMESVVSALTNPRAKSVLKGMETHNMGTQSFDSVAQKLSIYLPLA
                     KIASSDAHIYWAVGAGRTEFPGKTAQDLRTALEKNTTVPIPYHEEFSAKAILSWVRRV
                     TLRRFGYASDSISATQPINTQKLSESFIQKTKKKIIK"
     gene            complement(7142..8254)
                     /locus_tag="PL000_12485"
     CDS             complement(7142..8254)
                     /locus_tag="PL000_12485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC89764.1"
                     /translation="MIPGLDGLRAVAFLIVFFFHTRNLPFGWMGVQLFFVLSGFLITD
                     ILLRMKEKLPQREFFIKFYGRRFLRIFPLYYFYLLLLAGLVFVLPPLNIGLLNAELGK
                     SFINQIWVAAFYLYDFFHASVFFERSRFFTHLWSLSVEEQFYLLWPLLIFLVPRQKFK
                     QLCLTAIGLGFVFRLVITLVYRAGIFPFLLNDPQQAVNVLPFSHLDAFAFGAYISRFE
                     IPRPRLQLGLLAIFVPALGLLTDFLSKGSVVVASLGYDLPMTGFYKEVWGYTLLNYLF
                     AVLIHCVARTELLNSILESAPLRYLGKISYGLYVYHYGIIALAVAVFREYGLPYSVRS
                     PQMFVAAMGSTLLIATLSFYLLEKPIIDLKDKFFRI"
     gene            complement(8251..9399)
                     /locus_tag="PL000_12490"
     CDS             complement(8251..9399)
                     /locus_tag="PL000_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC89765.1"
                     /translation="MSKHDVLIIGGGPSGLSTALHLKKIDPHLASRILILEKEQYPRF
                     KLCAGGLVIDAEIILQRLGLDVTEVPHVNVDSIHFDFMGSGLKVHVPNRHALRVIRRN
                     EFDAWLAKKTLEAGIEIREGVAVMNVTPTDDGVTVETDQGVFFAQVVIGADGSNGITR
                     RCILPNAPISTARVLEVLTPAHPQVSRGREKDAFFDFFPVPNNIAGYVWDFPTLVNGE
                     TMRCWGVYDTNLLADEKRPALKEPLAREMARHGFDLNQFELKGHPIRWYAPQNQISVP
                     RVLLVGDAAGADAIFGEGISMALGYGAIAAQEVSESFRRSEFSFRRYRRRFARSGLGQ
                     TLFVRWVITNIIYPIKWKWFQILLWRIAKPIVLIIAWVFVLNWSKRIK"
     gene            9478..10284
                     /locus_tag="PL000_12495"
     CDS             9478..10284
                     /locus_tag="PL000_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89766.1"
                     /translation="MAAFKTFVKNMLLLLPNGERIVAWIVEKRRKPQPAPVKTPAPAV
                     VEPPVENKPADPRSVFTDYYFNNFWGNKESVSGAGSTMEYTENVRREIPLLMNRFGVK
                     KFLDAPCGDYNWFQAVQRPAGMEYIGGDIVEELIKQNQLKYGDASTRFISLDVITEPL
                     PSSEFWMCRDCLFHFSYDDIFKTLANFVRSDIQYLFTSIHTECTANADIVTGGARQLN
                     LELPPFNLCKPVLYVDDWIPGFTVRRMGVWTRQMVAESLASNAEAKKYLA"
     gene            10420..11097
                     /locus_tag="PL000_12500"
     CDS             10420..11097
                     /locus_tag="PL000_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC89767.1"
                     /translation="MLEELPSHRQLRKLVADQIRAAIFDGRFKPGEWLRQERVAQELG
                     VSQMPVREAMKELAAEGLIEHVPYRGVRVVALSMEDIEDLYEHRAFLEGRAAEKAVEY
                     ITDGEIMDLKLMVAEMESLPPEQIAQYRKVNRKFHETIFLISRREYLIRTLTQMWEAF
                     PTMLIGNFAVTAQQPLPQRDMQDQLEHRAIMDALEKHDGKKAGDAMKDHILSAARNLI
                     ESLKVSS"
     gene            11094..11909
                     /locus_tag="PL000_12505"
     CDS             11094..11909
                     /locus_tag="PL000_12505"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNC89768.1"
                     /translation="MTTTTLMPMGGALDERKDPTVLQEFLRRAGGKKARIVILPQASA
                     RRDTGKEYIELFKAFGAKEAVSFEFRKRSEAGTAEQIKLIRKASGIFFSGGTQMRIAA
                     AMGGTALEAEIHAAYHRGCIVGGTSAGASILSKTMIAYGNGGPTPRERIIQFSAGLGF
                     TDKFIFDQHFRQRDRLGRLIYAVSSHPGVIGVGIDEDTAAIVEDNARLTVAGSGAVTI
                     VDGFQIADTDVAEIEHGGAVAVSNLLVHVLTHNSVFDGRTRKAALPKKKLLID"
     gene            11938..13686
                     /locus_tag="PL000_12510"
     CDS             11938..13686
                     /locus_tag="PL000_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC89769.1"
                     /translation="MKFLKNVLVLMVVASFVLSACGSPAAEQPAETDAPAATDAPAAT
                     EAPAATEAPAVDPNAPVSGGTVIIGTPQEPGMMNTLLTSSSIEDAVVSLFGEGLVSTN
                     ENGEYVPVLAKELPTVSEDGLVVTWLLKEGIKWSDGSDFNCDDVRFTMEGALSDLSQV
                     SASGYRDIETLECPDPYTVVATFGEVYAPYLRLFSYTVPDTAGALEDMETWDINRNPV
                     GTGPFILTEWEPGDHLTFSRNPLYREAGKPYLDNVIIKILPSREVGMQLLGTGEIHAL
                     WDLTEANFPELEQLSSQGVSFVSTVTGENELLSLNFGANDGTAPADPATAPHPILFDL
                     KVREALQLAIDKQTIVDTLLYGNVKAGNSAVPVGTFGCPQPVSEFNLEKANALLDEAG
                     WVVGADGIREKDGVRMSLKIATTTGNQLREQTEQVLAEMLKAAGIELVIENVPSDVLF
                     ASWESDGMRDHGTYDIVMYTTGPSTDPDSHLYSSFHSSEIPTADNEGSGANFARYINA
                     DVDSWLDEAATITDTARRKELYCQVVEQVNKDLPRIYLYERLSIMGHRNEFHGLKISP
                     TFVDFAWDAANWWLSQ"
     gene            13872..14843
                     /locus_tag="PL000_12515"
     CDS             13872..14843
                     /locus_tag="PL000_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89770.1"
                     /translation="MAIYFTRRVLQSLFTLIVISMILYGLISSVPGGFMSAYTERSDF
                     TQEDLARIRAKFGLDDPIPVRYFKWLTAMLQGDMGNSFTTKRPVLQEIADRLPNTLLL
                     MGIMMVTTLLVAIPLGIISAVKQYTWFDHIMTTVAFAGQSLPVFWFGLLLIIIFAVTL
                     KGADGKPLLPGSGMYTLGEPFSIADRIKHLILPISMLTFVSAAGYMRYLRSSMLDVIH
                     EDYVRTARAKGLKERLVIYRHALRNAIIPLVTLVALDLPSLFGGALFTETIFAWPGIG
                     RLYFTAALKTDYPLVMAVLMIESALIIGSNLLVDFIYAALDPRIRLS"
     gene            14846..15769
                     /locus_tag="PL000_12520"
     CDS             14846..15769
                     /locus_tag="PL000_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC89771.1"
                     /translation="MAATSPFSSERPKSLREQSLFMFYWARFRKHTMALIGGVVTLLV
                     LILIVFAPLISNYDPMTQDLLNRFQPPSSQHWMGTDDLGRDMFSRVMYGGRISLSVGM
                     LAMAIAIFLGSTIGLISGYYGGWVDSILMRMTEIFMSIPSLFVLIALGMFLRTLDLPS
                     LKAGSFLPISIVIGALSWMGTARLVRASTLELREREFVHACRALGGSDTRILFLRILP
                     NIASPIIVSATLGLAGAIISESGLSYLGFGVQLPTPTWGNMLSNTQSQMTTAPWTAIF
                     PGLMIFIVVIAINYLGDGLRDALDPRHSVKK"
     gene            15836..17860
                     /locus_tag="PL000_12525"
     CDS             15836..17860
                     /locus_tag="PL000_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89772.1"
                     /translation="MKSRLVILTVLITLLFGIPQGISAQTSPGLLLPIGAGYTDTYTA
                     LGQYAVANAKGDVVHILVLASPYSTDAEHISEGERAQNLKDAEERRIQTEGACQRNLP
                     EGSTLTCKVELLPIFTQEDSNNPETLAYFTDDVSMIFILGGDQETGMGAIINTPVEER
                     INELHEMGTIIAGTSAGAAMQSKIMIASLNIGYATDDGLQAGALKLWDTEEKHGFSFS
                     IQNAVLDQHFFQRARMGRLVNVITQPGKPHVGVGVDAYTGVVSDNEVLRDVFGLYTVT
                     VLDAETYHSADAVKYVEFSETRPPLISVRNIILNLLSPGDVTYDLKTRTSSLGNPPDV
                     LERNFDALTLPAGAGPLFLGGDQLDTLKDSPILEKFKEVAGENILIVATGYPSSRSAE
                     TAIKKYTEGLGMQVQSLVVGDAPIVIPDDVTAVLVVGKDTSKVKVEALAPLKDFWLAG
                     NPVMADNAVTPVLGAFYSNHEPTPDDSELEELATQKSFWQGRTKMAPGLGFLKITIEP
                     QLLADLRFGRLFSLAYNHPEFIAIGVNKDTAIMLDENGATALGVNGIFVLDLRKAKLQ
                     LGTNDGFVINNGMLDIFAPGESIQAEIADVNARFDPAPTPALPTPVPTATATVAATPT
                     QPATATPSIVPTSPTAMEYAETQSPAPFPLWIIAVVVAIVSALVYFWKRK"
     gene            18116..20053
                     /locus_tag="PL000_12530"
     CDS             18116..20053
                     /locus_tag="PL000_12530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89773.1"
                     /translation="MPDLVGKSLGRYHIGEQLGEGGMAIVYKAYDTRLETDVAVKVIR
                     TDNILPSVLDSALKRFEREAKLLARLLHPNIVKVIDYGEFEGMPYLVMPYLPGGTLKR
                     KLGKPTSWQESFKLLLPIAEALDYAHSQNMIHRDVKPSNILLTQRGQPMMADFGIAKI
                     LDLENTQELTGTSMMIGTPEYMAPEQVTAKTVDHRADIYALGVVLYEMVTGRQPFLAD
                     TPMAVLLKHNSEPLPHPRQFVPNLPDAVEKVLLKALAKHPDDRYQSMEEFGAVMKRLL
                     QEPPPQFEAGSTWGTSRTQTVQSANGNSGWKILKWGVVAGLFCLVAGAGLFFVFQRLK
                     NLPVNSAMVSTPVAATETPSPVVPPTETPTSAPTPLPARIEDEKGVPMVLIPSGPFLM
                     GSLQERVEQDFATCEKETNGQCELSWFTNETPQHLVRLDDYYIDMYEVTNARYAECVA
                     ADVCVAPSPTSSFTRDEYYGLPEFADYPVIHVSWADGRLYCDWRGGRLPTEAEWEKAA
                     RGPDGLIYPWGNEFDGTKVNFCDSNCPIDWANKDYNDYFGDTSPVGYYESGKSPYGVY
                     DMAGNVWEWISDWYQEDYYSISPSFNPLGPETGQFRALRGGSWSNVSSAMPSSMRSWD
                     VVPSLAQFGYYGFRCARNANE"
     gene            20125..21693
                     /locus_tag="PL000_12535"
     CDS             20125..21693
                     /locus_tag="PL000_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89774.1"
                     /translation="MSQTPRPLKVFLCHSHSDRTAVRALYSRLVLDGVDAWLDKEKLL
                     PGQEWELEIRRAVRESDVVVICLSNQFNQAGFRQKEVRLALDTAMEKPEGEIFIIPAR
                     LEECESLASLSKWQWVDLFEENGYEKLLRALVLRSDRIRANARSLMRFDAKAEKRRQV
                     VFLVQQAEKMEKDADWMAAIENYRQVNRIDPAYFGVEEKIASLQQKMQAGTVTVTLRQ
                     DAVVDKPSSSKWLRVAGIVGVLLLCCMFVVAGGILLYSRSRKSSAQIAPSPVVATASP
                     SLPEPSPTPGQPAVVSTQPVTPEMVLIPAGDFNMGSEGGDADELPIHLVRLDAYSIDV
                     YEVTNAAYQACVDSSACKPPISTGSLTREEYYGVSEYENFPVINIDWDRASTFCAWRG
                     GRLPTEAEWEKAARGTDNRIYPWGDAPDCAKSNCTADTLEVGSFGEGKSPYGVYNMAG
                     NVWEWVADWYSREYYSISPLENPQGPSSGESHVLRGGAFDATERLKRVSARGAYFPED
                     FFSRVGFRCAADAP"
     gene            21761..23485
                     /locus_tag="PL000_12540"
     CDS             21761..23485
                     /locus_tag="PL000_12540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HNC89775.1"
                     /translation="MENFIGKHIDRYNIIDLLGQGGMATVYRAYDTRLEREVALKVIR
                     HEAFPPDVLQNELKRFEREAKSLARLSHPNIVKVLDYGEYEGSPFLVMEYLPGGTLKQ
                     KIGSPIAWQDAMHLLLPVARGVEYAHNREIIHRDIKPSNILLAENETPTLSDFGIAKL
                     FQNEGTLLTASGMAMGTPEYMAPEQWTGKTVPQSDMYALGVVLYEMVTGRRPYTADTP
                     GGVFMMQVTEPVLFPRDIVPHLPEAVEQFLLKALSREPSNRHASMTEFIRDMELLLSN
                     AETKVPINRMETLKGSPGQSSVRKDASSPSRKLVPFNWKAATLGGVILLAVVVAAAVV
                     LMKKKSTPLAAEITDSKNAVMVLVPEGDFVMGSDNGDEDERPVHTVYLDEFYIDKYEV
                     TNSDYRVCVVDGVCKPPQDMTNYNNPEYENHPVVYVDWEMARTYCDWRDAQLPTEAQW
                     EKAARGMDARTYPWGEGIACSQANYLSCIEDTQPFDSYAGSLSPYGAYNMAGNVWEWV
                     ADWYSPAYDVSETDNPVGAESGERRVLRGGGWNQHEYLLRTSARLGVVPTDVFLSFGF
                     RCASDVNQ"
     gene            23507..23878
                     /locus_tag="PL000_12545"
     CDS             23507..23878
                     /locus_tag="PL000_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89776.1"
                     /translation="MDASSPVQPNPQKRSNRPIAALLLSLSSFFFCCISVLVNNPNGF
                     DAFMEGKPSWYIAQALMCLAALLPLIGAILGILSLRAGEINRNLAIAAIVLGVIGFFE
                     SLGIFGYVMVFVGIINVFSPP"
     gene            complement(23958..24296)
                     /locus_tag="PL000_12550"
     CDS             complement(23958..24296)
                     /locus_tag="PL000_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNC89777.1"
                     /translation="MSRDESLYLADVQESCEKVLRFTKGMTYKDFVNDDLHFDAVLRN
                     LEIIGEAVKNISEATRQKYPDVKWRKIAGFRDIVAHEYFGINDETVWDIVENEVPALL
                     AVMKIMLEEK"
     gene            complement(24283..>24607)
                     /locus_tag="PL000_12555"
     CDS             complement(24283..>24607)
                     /locus_tag="PL000_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC89778.1"
                     /translation="WLFFLLYNLNMTRRTVITRIRKHRAQLTKLGVKSLSLFGSVARG
                     EEGPNSDVDILVEFKGKATLDRYMDTKFYLEDLLGCKVDLVTPKAIKPRMKPYIMQDL
                     VHVTG"
BASE COUNT         5775 a         6298 c         6482 g         5952 t
ORIGIN      
        1 aggtggcgca attcgcattt ttgttgttgg aagttataat tgtttgagaa agaagcgaga
       61 ggcagtataa tcgccgtaat tcattctcag gagcaggctg tgacaaaaac tgaccggaag
      121 cctttgttgg aagtccgaaa tcttaagacc tatttttata cagaagacgg ggtggtaagc
      181 gccgttgatg gcgtcagttt tgaagtgtat cccggtgagg tattgggtat cgttggagaa
      241 tcgggttgtg gaaaaagcgt gacctcgctt tccatcatgc gcctgatcgc accgcccgga
      301 aagatcactg aaggggaaat tttgctggat ggcaaaaacc tgctcaacct ttctgaagac
      361 gaaatgacaa aaatgcgcgg caacaagatc tcaatgatct tccaacagcc gcagaccgct
      421 ttgaacccgg tgttcaaggt ggataaccag attgccgaag tattaaatat ccacaaagca
      481 gttggcaagg aagcaggccg acagcgtgcc atcgaattgt tgaagatggt ggggatccca
      541 gatgccgagc gccgcgccga tgcgtatccg cacgagcttt ccgggggcat ggctcaacgt
      601 gtgatgatcg ccatggcgct ggcttgtacg ccggaactgt tgatcgcgga tgaacccaca
      661 actgcgcttg atgtgaccat tcaggcccag atccttgacc tgatgcgcga tttgcgcaca
      721 aagatgggga cctcggtcat tttgatcaca catgatcttg gtgtggtggc agagatggcc
      781 gaacgtgtgg cggtgatgta tgcaggtgaa gtggtggagc aggccgaagt gaattcgctt
      841 ttcgagcaac cattgcatcc gtacactcag ggcttgatcg gttccattcc ggtccttggc
      901 cagatcaagg aacgcctcga tgtgattcca ggttccgtgc ccaatttggt gaacctgccg
      961 cccggctgcc gctttgcgcc gcgttgtacg gctcgtgaaa aattcggctt aaagatttgc
     1021 accgaagtaa aacccgagct gactgatgcc gcccccggtc acaaggttcg ttgctggctg
     1081 tatcaaaacg caaaagatca ccaagcgccg ttgaaggcca agcagggtta gttttttagg
     1141 cggccgtttg gggaggttgc cgcgttatcg taaacaatcg tctggagtcg atgagcatga
     1201 gtcaaacaac ggaaatgcag aaaacagaag acctggtaca agttgaacat ttggtgaagt
     1261 acttcccggt acgcgccggg ttgatgcagc gcgtggtcaa ttgggtaaag gctgtggacg
     1321 atgtttcatt cactgtgaaa aaaggggaaa cccttggcat ggtgggagaa tcgggctgtg
     1381 gaaagaccac cattggccgt tccatgctca ggctggtgga gccgacctcc gggtctgtgc
     1441 aatacgacgg gcgggatgta ttgaagttgc gcggcagtga attgaaggat gtccgccgcc
     1501 atatgcagat cattttccag gacccgtacg cttctttgga tccgcgtgtg cccattggcg
     1561 aatcggtgat ggaaggtttg catattcaca agatcggttc gccgcaggaa cgctacgacc
     1621 tgatgatcga aaccctcaaa aaggtcggtc ttgaagatta tcacgcccgc cgctatccgc
     1681 atgagttttc cggtggtcag cgtcagcgca ttggtattgc gcgtgcgctc gcgttacgcc
     1741 caaatttcat tatttgtgat gaacctgtct ctgcgttgga tgtatccatc caatcgcaag
     1801 tgttgaatat tttgaaagac cttcaaaagg aatttggtct cacctacctg ttcatcgccc
     1861 acaacctgag cgtggttgag catatttcag accgtgtggc tgtgatgtac cttggcaaaa
     1921 tggtggaatt gacctcccgc gacgacctct tcaagaatcc gttgcatcct tataccaagg
     1981 cgttgatgtc tgccattccg atccccgacc cgaaactcaa acgccagcgt gaagtgttga
     2041 agggtgatgt gcccagtccg ttgaacccac ccaagggctg ccgtttccac ccgcgctgcc
     2101 ccattgccga caaaatctgc tccgagcagg agcccgaatt ccgtgaagct gctgccggtc
     2161 attttgtcgc ctgttggatg gtgaagtagg aaaatatgga tcgcaatatt ttgcttgtgg
     2221 atgaccagcg cgatatcctg cgtcttttgc gttccacact tgaaacgctc aaaaaaacgg
     2281 agatcaagat attcgaagcc ccttcaggcg aagaagcctt gatcgagtcg tcccgccaca
     2341 aggtggatct gcttgtcaca gattacaaac tgcccggtat gagtggtttg gaattgatgc
     2401 acaaggtacg tgtgcgccat ccggaagcaa aagttatttt gatcactggt ctgaccgacc
     2461 gcaaagtacg tgaagaaatg ctcaacgccg gtgcacttgc ggtgttcgat aaaccgattc
     2521 ccctcgccga ttttttggat gtagttgaac gcggacttgg attcgttcaa accattttcc
     2581 ccccagaacg cccctccgac gaaaaaacag aacgacgtca tgcccgcgta tctgacctgc
     2641 tggcaaattt cagacaggat attaacgccg aagcagtctt ccttttgaat gaacgcgggc
     2701 ttgtacaggc ccgcaccggc aaactgcgcg acgaaagcat ggaagtttcg ctcttctcca
     2761 ctttgatggc gattcacaac gcgagcctta aagtggcaaa gtataaccat caggaaaaac
     2821 tcgattccta tcatgtgttc agcagcggcg ataatgacct cctgttcatc cctgtcgcac
     2881 ccatgtatgc cttgctggtt gcagggaatg gacttgtccg tgaagatcgc atccttgaat
     2941 ctgtgaacgc cctgcttgct ttacgcaagg aagtggaacg atctctgcgc tcgcttgggg
     3001 tcaccggcga gttgcagtca atttccccga agaccgttcc tctggcgacg gcagcggctc
     3061 ccaccatccc gatacctgct tctattaaac cgatggagaa acccgctgaa gcagcccctt
     3121 cccccgagct tgaagcccta ttgaagaatg ccgctgccac aaaagaccaa accgccaaca
     3181 tggatgattt ctggaatcag gctgcagaaa aacacggcaa caaacctgcc agcaaggaag
     3241 tgatctcact tgaagatgcc cgcaaactgg ggctgcttcc tggagatgga aagtaaaaaa
     3301 tttgtgatac aattttgccc gcactggggc atggtgcaat ggcagcacac cagcctttgg
     3361 agctgtggat cttggttcga atccaggtgc cccagccata catcccgctt acgcggggat
     3421 tcttttctca aataggattt gtcgtcgagc aatgcattgc cgcgcaaatc ctattttcgt
     3481 tattccctgt tcgtttcgag tgaagtagca agacgcatat ccttatcgag gtgacatcat
     3541 gaaagtgacc gcagttctcc ttgccgccgg gcagggaacc cgcatgaaat caagcttgcc
     3601 caaggtttta catcccattg caggcaggcc gctcatctgg catgccatgc aggcgatcag
     3661 tcagtccacc acagagaagc caattgtagt ggtggggcat ggcgcagaag aagtgacgaa
     3721 atatctgggc gattctgcac aaaccgtttt acaggaaccg caactgggaa ccggccatgc
     3781 agtgatgcag gccgaggacc tattaagagg caagaccgat ctggtagtcg tttgctatag
     3841 cgatatgccc ctgctccgtg gagagacctt gcaggcattg gttgaaacgc agaagaagaa
     3901 caaaggaccg atctccatgc tcaccgttca tgcggatgac ccgcgtggct ttgggcgtat
     3961 catccgcaag acggatggta ccgtggatgc cattgtggag gaatatgtcg cgacgcctga
     4021 gcagttacag gtcaaggaat tgaacgtggg cggttattgt ttcgatgcca attggctttg
     4081 ggatgccttg caccgcattc caaagaatcc caagaagggt gaatattatc tgaccgacac
     4141 agtggaattg gcttcccagg ctgggttgcc agtgcaggcc acggtgatgg atgatgtcga
     4201 agagaccatc ggaattaata cccgcatcca tcttgcggat gcggaagctg ccatgcgccg
     4261 ccgaattaac caacagcata tgttgaacgg tgtcacaatg atcgatcctg cttccacata
     4321 tattgaagca ggggtaagca tcggcaggga tacggtcatc atgccgaata catatttgca
     4381 tggcaggaca gagatcggcg aaggaaatgt gatcggaccg aacaccatta tccatgattc
     4441 aaagatcggg aatcattgca aggtactggc ttcggtgatg gaaggcgccc ttcttgatga
     4501 ccatgtggat atgggaccgt tcgcccgttt gcgcaagggg gcgcatttgg ggaatcatgt
     4561 tcatatgggg aactttggtg aagtgaagga ttcgtatctt gcagatggtg tgaagatggg
     4621 acatttttca tacatcggca atgcccagat cggttcgaat accaatatcg gtgcagggac
     4681 catcacctgt aactatgatg gcgaaaagaa acatcctacc gagatcggtg aggatgtatt
     4741 catcgggtct gataccatgt tggtggctcc cctgaagatc ggcgcaggtg cgcgaacagg
     4801 cgcaggtgct gtcgtgacaa agaatgtttc ggaagatacc ctggtggtag ggatgcccgc
     4861 ccgggcgatc aagaagttgg agcgtaaaac gaagaagtag gccagaaaga aaatggacag
     4921 gtgaactatg gatctaacca atgatgaacg acagacactg ataaaacaag ctgttgaaat
     4981 tcgcaaacga gcttacgtcc cgtattcaaa ttatcccgtt ggggcagcag tgcgaacaaa
     5041 atcgggaaag gtgttcaccg gcgtgaatat tgaaaatgcg gcatatccgc agaccatgtg
     5101 tgccgagcgg gtggcgattt tcaaggcggt gtctgagggt gagtctgaat ttgatgtgat
     5161 cacagtggtg accaataatg gcggttcccc ctgtggcgga tgcagacagg tgatggctga
     5221 attcggcctg gatacagttg ttctgatcgc agatggggat ggaaacttgc ataaagaatt
     5281 aacggtggcc gatctgctgc ctgaggcatt tacgccaaaa catttgcagt aaagtataag
     5341 gtgttgcatg gtaacgagcc gcgctcgttg ctgtgaatta tctcccatga ccgaattccg
     5401 ttcgtttcgt gcttctgctg ttgttgtacg ccacaccgat tggggtgagg cggaccgttt
     5461 gctgacgctg tacacacgtg aacagggaat ggtaaaggca cttgccaaag gtgcgcgaaa
     5521 gatcaattca cgcaaggcgg ggcacttgca gccgttcacc catgtcactg tgcaattggc
     5581 gaaggggcgt gacctgttga ttgtgaccca ggctgaaacc gtggatgcct ttttgccttt
     5641 gcacgataac ctggtgaaga caggttatgc tgcttatatc gttgaattgc tatatcggtt
     5701 ttcctatgaa gaggaaggcg ggaatccatc gctcttcaag ctgttggtgg atactttggg
     5761 caggatcgaa aaagaggacg aagcctggct gccggtccgt tattatgaga tgaggctgct
     5821 ggatattgtc ggctttcgtc cacagttgtt cgagtgtgcg aattgtggac gggagatctt
     5881 gcctgaagat caatactttt cgtttgtgac ggggggggtg atttgtccgc agtgcgggtt
     5941 ggggttgccc aatctggcga agatctccat ggaagcgctt aaatattttc ggcacttcca
     6001 aaggtcaagt tacagagatg cttctcgcgc acatcccagt ttcgaggtcc agaaggaagc
     6061 ggaagccttg atgcagggct atttcaccta tttgcttgag cgcgaattga atacgccggg
     6121 ttttatcaaa cgggtcaaat catgaagaaa atcgtttttg tgcgggggga atttccacac
     6181 aggtcacata ctttgacggc tgcgttaaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn ttttattatt ttcttttttg ttttttgaat aaaggattcg gacagctttt
     6361 gtgtgttgat gggctgcgtt gcagagatac tgtcagatgc gtagccaaac ctgcgcaaag
     6421 ttacgcggcg cacccagctg aggatggcct ttgcggagaa ttcctcatga taagggatgg
     6481 gaactgtggt gttcttttcg agtgcagtgc gaaggtcctg cgcggttttg ccggggaatt
     6541 cggtgcggcc tgctccaacc gcccaataaa tatgagcgtc gctggatgca atctttgcca
     6601 gcggcaggta aatggaaagt ttttgcgcca ctgaatcgaa tgattgcgta cccatgttat
     6661 gcgtttccat gcctttgagt acgctctttg cgcgcggatt ggtgagcgca ctgacaacgg
     6721 attccattga gagactgcct ggcaggttat taaatgggtg tggagcgatc gcgatgccgc
     6781 cctgtttccc gatgtggatc agcgtatcga caagtggaag ccccgcaggc ggcagggttt
     6841 ccacaaaaag tgccacaagg tgaccatctt tggtggtgac ttctgcgccg gggatgactt
     6901 caaccccgta cttgggtgcg agttcgcagg cttcaagcga accgcggatt tcatcgtgat
     6961 cggtaacggc gatcacatcc aggccgacat ctgcggcttg tttgagaatg gcacgtactg
     7021 tagtggtggc gtcgtgtgaa tagatgctgt gaatatgcag gtctgcagtg cccatggcaa
     7081 tgtgttttcc ctgaaagtat tatacgttga aagttccaaa aaggaagatg ataaatgtta
     7141 tttagattcg gaagaattta tccttcaggt ctatgatcgg tttttcaagc agataaaaac
     7201 tcaacgtggc gatcaatagg gttgacccca tcgcggcgac gaacatttgc ggtgaccgca
     7261 cagaataggg caggccgtat tcccggaaga ctgcaaccgc cagtgcaatg atcccatagt
     7321 gatagacata caatccatag gaaatctttc caagatagcg caaaggggcg ctctccagta
     7381 tggaattgag cagttcagtg cgtgccacgc aatggatcaa cacggcgaaa agataattca
     7441 gcagagtgta gccccacacc tccttgtaaa agccggtcat cggcaagtcg taacccagtg
     7501 acgcgacaac aacggagcct tttgagagaa aatctgtcag cagaccgagg gcagggacga
     7561 aaatcgcaag cagacccagc tgcaagcgcg gacgcggaat ttcaaagcgt gagatgtacg
     7621 caccaaaagc aaacgcatca aggtgggaga acggcaaaac gttcacggct tgttgcgggt
     7681 catttaacag aaacgggaat atccccgcac gatagaccag ggtaatgacc aaccgaaaga
     7741 caaatccaag cccgatggcg gtaaggcaca actgcttgaa tttttggcgc ggcacaagga
     7801 aaatgagcag cggccacaac aaataaaact gttcttccac cgagagcgac caaagatggg
     7861 taaagaaacg gcttctctca aaaaaaacgc tggcatgaaa aaaatcatac aggtaaaaag
     7921 ccgccaccca aatttgattg ataaaggatt tgccaagctc cgcattcagc aaccctatgt
     7981 tcaacggcgg tagtacaaaa acaagccctg caagcaggag caggtaaaaa taataaagtg
     8041 gaaagatgcg taaaaaacgc cgcccgtaaa acttgatgaa aaattcgcgc tgcggcaact
     8101 tctctttcat gcgcagcaaa atatctgtga tcagaaaccc cgaaagcaca aagaacaact
     8161 gcactcccat ccaaccaaag ggcagattgc gtgtgtgaaa aaagaagacg atcagaaatg
     8221 ccaccgcccg taaaccgtcc agccctggga tcatttaatt cgtttgctcc aattcagcac
     8281 aaagacccat gcaatgatga ggacgatggg tttggcaatt ctccataaca ggatttgaaa
     8341 ccacttccac ttgatgggat agatgatatt cgtaatgacc cagcgtacaa acagcgtttg
     8401 accgagtccg cttcgcgcaa accgacgcct gtatctgcgg aatgaaaatt cgctgcgccg
     8461 aaacgactcg ctgacctcct gcgctgcgat cgctccataa cccagcgcca tgctgatgcc
     8521 ctcgccaaag atcgcatctg cccctgcggc atcacccacc agcaacaccc gcggcacaga
     8581 gatctgattc tgcggcgcat accagcggat gggatgcccc ttaagctcaa attggtttag
     8641 atcaaagcca tgacgtgcca tttccctggc aagcggttct ttcaaggcag gccgcttctc
     8701 atctgcaagt agattggtat cgtacactcc ccaacagcgc atggtttcac cattgaccag
     8761 ggtggggaaa tcccaaacat agcctgcaat attatttggc acagggaaga aatcgaaaaa
     8821 tgcatctttc tcccgtcccc ttgatacttg agggtgtgcg ggagttaaaa cttccagcac
     8881 gcgggcggtg gaaatgggcg cgtttggcaa aatacacctg cgggttatac cgttcgagcc
     8941 atccgcgccg atgacgacct gggcaaaaaa aacaccctga tcggtttcaa cggttacacc
     9001 gtcatcggtc ggagtcacgt tcataaccgc cactccttcg cggatctcaa tgcctgcttc
     9061 aagggttttc ttcgcaagcc aggcatcgaa ttcattgcgg cgaatgactc gtaatgcatg
     9121 cctgttcggt acatgcacct ttaggccgct gcccatgaaa tcgaaatgga tgctatccac
     9181 gtttacatgc ggcacttcgg ttacatcgag gccgaggcgt tgcaggatga tctccgcatc
     9241 aatgacaagt ccgcctgcac acaacttgaa gcgcggatat tgctcctttt ccaggatcag
     9301 gattcgggag gcgagatgcg ggtcaatctt ttttagatgg agggcggtgg agagtccgct
     9361 cgggccgccg ccgatgatga ggacatcgtg ctttgacatg ccgctgattt tcccacaaaa
     9421 ttgctttgaa agggatatat aatgggcggg catttgaatt catttcaagg actccctatg
     9481 gctgctttca agacctttgt gaaaaatatg cttttgcttc tgccgaacgg cgaacggatc
     9541 gtggcatgga tcgtcgaaaa acgacggaaa ccacagcctg ctcccgtgaa aacgcctgcg
     9601 cctgctgtgg tggagccgcc cgttgaaaac aaacctgccg atccccgcag cgttttcact
     9661 gattattatt tcaacaactt ttggggtaac aaggaaagtg tctcgggggc tggttcaaca
     9721 atggagtaca cggaaaatgt ccgcagggag attccgctgt tgatgaatcg ctttggcgtg
     9781 aaaaagtttt tggatgcccc ctgcggcgat tacaactggt ttcaggctgt tcagcgccct
     9841 gctggcatgg aatacattgg cggcgacatc gtggaggagt tgataaagca gaaccaactc
     9901 aaatatggcg atgcctccac ccgctttatt tcactcgatg tcatcaccga acccctgcct
     9961 tcttctgaat tctggatgtg ccgcgattgc ctgttccatt tttcttatga tgatattttc
    10021 aagacgctgg cgaattttgt gcgcagcgat attcagtacc tgtttacgtc cattcatacg
    10081 gaatgtactg ccaatgccga tattgtgacg ggaggggcgc gtcagctgaa ccttgaattg
    10141 cctccattca acctgtgcaa acccgtttta tatgtagatg actggattcc aggctttacc
    10201 gtaaggcgta tgggggtgtg gactcgccaa atggtggcgg aaagccttgc ttcgaatgct
    10261 gaggcgaaaa aatatctggc gtaattcttg aaaaaacggc tcgtttgagc cgttttttat
    10321 ttcctgcata actggtgacc ttaaaatacc ggggaatgct tgactcttgc ctgttggtat
    10381 gctatcttag atattatatc taatatcaag gacaggccca tgcttgagga acttccctcc
    10441 catcgccagc tgagaaaact tgtcgcagac caaattcggg ctgccatttt tgatgggcgc
    10501 ttcaagccag gcgagtggct gcgtcaggaa cgggtggctc aggagttggg ggtcagccag
    10561 atgccggtgc gtgaggcaat gaaggaactg gcggcagagg gcttgattga gcatgtaccc
    10621 tatcgcgggg tgcgtgtggt ggcactctcg atggaagata tcgaagattt gtacgagcat
    10681 cgtgcctttc ttgaaggacg cgcggctgaa aaggccgtgg aatatattac cgatggggaa
    10741 atcatggatt tgaaactgat ggtggctgaa atggaatctc tgccgccgga acagatcgcc
    10801 caataccgca aagtcaaccg taaatttcat gaaacgatct tcctgatcag caggcgtgag
    10861 tatttgatcc gcacactgac tcagatgtgg gaggcctttc ccaccatgct gatcggcaat
    10921 tttgccgtaa cagcccagca accgctgccg caacgcgata tgcaggacca attggaacac
    10981 cgcgccatca tggatgcatt ggagaagcat gacggtaaaa aggcaggcga cgccatgaaa
    11041 gatcacatcc tctctgcggc acgcaacttg atcgaatccc tcaaggtgag ttcatgacca
    11101 caacaacatt gatgcccatg ggcggcgcac ttgatgaacg caaagacccg accgtgctgc
    11161 aggaatttct gcgccgcgca ggtggaaaaa aggcgcgcat tgtcattctg ccgcaggctt
    11221 cggctcggcg cgatacgggc aaagaatata ttgaactgtt caaagctttc ggcgccaaag
    11281 aagcggtctc ttttgaattt cgcaaacgtt ccgaggccgg cacagccgaa cagatcaagt
    11341 tgattcgcaa agccagcgga atctttttct ccggtggaac ccaaatgcgg atcgccgctg
    11401 ccatgggcgg tacagccctc gaagcggaaa tccatgcagc ttatcaccgc ggatgtatcg
    11461 ttggcgggac cagcgccggg gcatccattc tttcaaagac catgatcgcc tacggaaatg
    11521 gcggtccgac tccccgtgag cggatcatcc agttttcggc ggggcttggt ttcaccgata
    11581 aattcatctt cgaccagcac ttccgtcagc gcgaccgatt gggtcgcctg atctatgccg
    11641 tatcttcaca ccccggtgtg atcggtgtgg ggattgacga ggacaccgcc gccatcgtgg
    11701 aagataatgc ccgtttgaca gtggcaggtt cgggtgcggt cacgatcgtg gacgggtttc
    11761 aaatcgccga tactgatgtg gccgagatcg aacatggcgg cgcggtggcg gtttccaatc
    11821 tgcttgtgca tgtgttgacc cataacagtg tgttcgacgg gcggacgcga aaagcggccc
    11881 tcccgaaaaa aaagctcctg atcgattagg gagcatagat caaaaaagga gaagattatg
    11941 aagtttttga agaacgtttt ggttttgatg gttgttgcat cgttcgtgct ctctgcttgt
    12001 ggcagccctg ccgctgagca gcctgctgaa accgacgccc ctgctgcaac cgatgccccg
    12061 gccgcgactg aagcccccgc cgcgaccgaa gcccctgctg ttgatccgaa tgctcccgtc
    12121 agtggtggta ctgtgattat cggtacaccg caggaacctg gcatgatgaa cacgctgttg
    12181 acctcctctt ccattgagga tgcagttgtg tccttgtttg gtgagggtct tgtctccacc
    12241 aacgaaaatg gtgagtatgt tcctgtgctg gccaaggaat tgccgaccgt ttctgaagat
    12301 ggcctcgttg taacctggct tttgaaggaa ggcattaagt ggtctgatgg ttccgatttc
    12361 aactgcgatg atgtgcgctt taccatggaa ggtgcccttt ccgatctttc tcaggtgagc
    12421 gcttcaggct atcgcgatat cgaaaccctc gaatgccccg atccctacac cgtggttgcg
    12481 accttcggtg aagtgtacgc cccctacctg cgcttgttct cttacaccgt gcccgatacc
    12541 gccggtgcct tggaagacat ggaaacctgg gatatcaacc gcaacccggt tggtaccggt
    12601 ccgttcattt tgaccgaatg ggaacctggc gatcacctta ccttcagccg caatccgctg
    12661 tatcgtgaag caggcaagcc ctacctcgat aacgtcatta tcaagatcct gccttcccgc
    12721 gaagtgggta tgcagttgct cggcacgggc gaaatccacg cactgtggga tctgaccgaa
    12781 gccaacttcc ccgaacttga acaactttct tcacagggtg tttcgttcgt ctccaccgtg
    12841 accggtgaaa atgaactgct ctccctcaac ttcggtgcca atgacggtac tgcccccgct
    12901 gaccccgcca cggctcccca cccgatcctg ttcgatttga aggtgcgtga agcgttgcag
    12961 cttgccatcg acaagcagac gatcgtggat accctgcttt atggcaatgt gaaggctggt
    13021 aacagcgccg tacccgtggg taccttcggc tgcccgcagc ctgtcagcga attcaacctt
    13081 gaaaaggcca atgccctcct ggatgaggct ggctgggttg ttggcgctga tggaatccgt
    13141 gagaaggatg gcgttcgcat gagcttgaag atcgccacca ccaccggcaa ccaactccgc
    13201 gaacagaccg agcaggtact ggctgaaatg ctcaaggccg ctggcattga actcgtcatt
    13261 gaaaacgttc cctccgatgt gttgttcgca agctgggaga gcgatggtat gcgcgatcac
    13321 ggcacctacg atatcgtgat gtacaccacc ggtccttcca ccgacccgga tagccacctg
    13381 tactccagct tccacagctc tgaaatcccc accgctgaca atgaaggttc cggtgccaac
    13441 ttcgcccgct acatcaatgc cgatgtcgat tcctggcttg atgaggctgc caccatcacc
    13501 gataccgccc gccgcaagga actgtattgc caggtcgttg aacaggtcaa caaagacctg
    13561 ccccgcatct atctgtatga acgtctctcc atcatgggac accgcaatga attccacggt
    13621 ttgaagattt cccccacctt cgtggatttc gcctgggatg ccgccaattg gtggttgagc
    13681 cagtaatcta aagtgtattt tgcccctcct cttatcatgg gaggaggggc aatccttttg
    13741 aaatcttcga tcttcacttg agttttttag tcaaagtata atttcggcat ctcaatggat
    13801 ggatgcatcc atccaccaaa aaggccgcgg gtctttttgg ataaaacagc aatctgggta
    13861 aagtcatcat tatggccatt tatttcaccc gtcgtgttct gcaatcatta tttacattga
    13921 tcgtcatcag tatgattttg tatggcctga tatcctccgt gcccggcgga ttcatgagcg
    13981 cctatacaga acgctctgac tttacacagg aagacctggc gcgtattcgt gcgaaatttg
    14041 gccttgatga ccccattcct gtccgctatt tcaaatggtt aactgccatg cttcagggtg
    14101 acatgggaaa ttcgttcacc acgaaacgcc ccgtgcttca agaaatcgca gaccgcctgc
    14161 cgaacacctt gcttctgatg ggcatcatga tggtgaccac tttgctggtt gccattccat
    14221 tgggtattat ttcagcggtg aagcaataca cctggtttga ccatatcatg accacggttg
    14281 cctttgcggg gcaatccctg cctgtattct ggttcggact tttgctcatc attatatttg
    14341 ccgtcacctt gaaaggtgca gatggcaaac cgcttctgcc ggggtcgggc atgtatacgc
    14401 tgggtgaacc cttttcgatt gccgaccgca tcaaacacct cattctgccg atctccatgc
    14461 tgacctttgt ttccgcagcg gggtatatgc gttacctgcg ttcttccatg cttgatgtca
    14521 ttcatgaaga ttacgtccgt acggcgcgtg ccaaaggttt gaaggaacgc ctggtcattt
    14581 accgccacgc cctgcgcaat gccattattc cgctggtgac gttggtggca cttgacctgc
    14641 cttcattatt tggcggcgcg ttattcactg agaccatttt tgcctggccg ggcatcgggc
    14701 gcttgtattt cactgccgcc ctcaagaccg attatccgct cgtgatggcg gtgctgatga
    14761 tcgaatcagc tctcatcatc ggctcgaacc tgcttgttga cttcatttat gcggctcttg
    14821 acccgcgcat tcggttatcg taaccatggc agccacttcc cctttttcct ccgaacgtcc
    14881 aaaatccctg cgtgaacaat ccctgttcat gttctattgg gctcgtttcc gcaagcacac
    14941 catggctttg atcggtggcg tggtaacgct ccttgtcctc attctgatcg tgttcgctcc
    15001 actgatctcc aattacgacc cgatgacaca ggatctgctc aatcgctttc aaccgccctc
    15061 cagccagcac tggatgggca ccgacgatct tgggcgtgac atgttctcac gtgtcatgta
    15121 tggcggccgc atctctttaa gtgtgggcat gcttgccatg gccattgcaa tttttctcgg
    15181 ttccaccatc gggctgatct ccggttatta cggcggctgg gtggatagca tcctcatgcg
    15241 tatgaccgaa atcttcatgt ccattcccag tttgttcgtg ctcattgcat tgggcatgtt
    15301 cctgcgcaca ctcgacctgc ccagtttgaa agcgggttcc ttcctgccga tctcgatcgt
    15361 catcggcgcg ctttcatgga tgggaaccgc ccgccttgtc cgtgcttcca cgcttgaact
    15421 gcgcgaacgc gaattcgtac acgcctgccg tgctttgggc ggttccgata cgcgcatcct
    15481 ctttttgcgg attctgccga acattgccag ccccatcatc gtttctgcaa ccctgggttt
    15541 ggctggcgcc atcatcagcg aatcgggtct ttcatatctc ggctttggtg ttcaacttcc
    15601 aacacccact tggggcaaca tgctttcaaa cactcaaagc cagatgacca ccgctccgtg
    15661 gaccgccatc ttccccggcc tgatgatctt catcgttgtc atcgccatca actatctcgg
    15721 tgatgggttg cgcgatgccc ttgacccccg ccattctgta aagaaataga ccgcggcaat
    15781 ttttgagtat aagcctgctg cgctttgtga tccacgattt tgggagagac tgaaaatgaa
    15841 atcacgcctc gttattttga ctgttttaat caccctgctg tttggaattc cgcagggaat
    15901 ctcggctcaa acttcccccg gcctgcttct gcccatcggc gctggctaca ccgacaccta
    15961 taccgccctc ggacaatacg ccgtcgccaa cgccaaaggg gacgtggttc acatcctcgt
    16021 acttgcctct ccctactcca ccgatgcgga acatatttcc gaaggtgaac gcgcccaaaa
    16081 cctgaaagat gctgaagaac gccgcatcca gaccgaaggc gcctgtcagc gcaaccttcc
    16141 cgaaggctcc acgctcacct gcaaagtaga gctcctgccc attttcaccc aggaagactc
    16201 caacaaccct gaaaccctcg cctatttcac cgatgatgtt tcgatgatct tcatccttgg
    16261 cggcgaccag gagaccggca tgggggccat catcaatacc cccgtcgaag agcgcatcaa
    16321 cgaactgcat gaaatgggaa ccattattgc gggcacaagc gccggggcgg caatgcaatc
    16381 gaagatcatg atcgcctcgc tcaatatcgg ttatgccacc gatgatggac ttcaggcagg
    16441 cgcattgaaa ctctgggata ccgaagaaaa gcacggtttt tccttcagca ttcaaaacgc
    16501 cgtcctcgac cagcatttct ttcaacgtgc ccgcatggga cgccttgtca acgttatcac
    16561 ccagcccggc aagcctcatg tcggcgtggg tgttgatgcc tataccggtg tcgtttcaga
    16621 caatgaagtc ttgcgcgatg ttttcggcct gtacaccgtc actgttttgg atgcggaaac
    16681 ctatcactca gccgacgccg tgaaatatgt cgaattcagc gaaacccgcc cgccgctcat
    16741 cagtgttcgc aatatcatct tgaacctgct ttccccgggt gatgtcacct acgacctcaa
    16801 gacccgcact tcatcactgg gcaatccgcc tgatgtgctt gaacgcaatt ttgatgccct
    16861 gaccctcccc gctggtgcag gtccgctctt cctcggcggc gatcaactcg atacgctcaa
    16921 agacagcccc attctcgaaa aattcaagga agtggcgggt gaaaatatcc tcattgttgc
    16981 cacggggtat ccatcatcac gctctgcaga aaccgccatc aaaaaatata ccgagggtct
    17041 tggcatgcag gttcaatcgc tggtggttgg tgatgccccc atcgttatcc ctgatgatgt
    17101 caccgccgtg ttggtcgtag gcaaggacac atccaaggtc aaggtggaag cgcttgcacc
    17161 gctcaaggat ttctggctgg cgggtaaccc ggtcatggcg gataacgcgg tcacgcccgt
    17221 cctcggtgcg ttttattcca accacgaacc cacccccgat gattcggaac tggaagagct
    17281 tgccacacaa aaatccttct ggcagggacg caccaaaatg gcgcccggcc ttggcttcct
    17341 caagatcacc attgaaccgc agttgctggc tgacctgcgt tttggccgcc tgttctcact
    17401 ggcctacaac caccctgagt tcatcgccat cggtgtcaac aaagataccg ccatcatgct
    17461 tgatgagaac ggcgccactg ctctcggcgt caacggtatt ttcgtcctcg atcttcgcaa
    17521 ggcaaaacta cagcttggca caaacgacgg ctttgtgatc aacaatggaa tgctggatat
    17581 cttcgccccg ggcgaatcca ttcaagcgga aattgccgac gtcaacgccc gcttcgatcc
    17641 cgcgccgacg cctgccctgc caaccccggt gccgacagcc accgcaacag ttgccgccac
    17701 acccacccag cctgcaaccg ccactccctc catcgtgccg accagcccca ccgcaatgga
    17761 gtatgccgaa acccaaagcc ccgccccctt cccgttgtgg atcattgcag tggtggtggc
    17821 catcgtctcc gccctggtat atttctggaa gcgtaaatag aaccaacccg attctcaaac
    17881 agccaacccg acaaaggttg gctgtttttt tatattttgc tggatatgga aaaggtgaat
    17941 cacgtaagtc gaattgcaaa ttcgacttac gctgtgccac agcgattccc aatgaaccaa
    18001 aagcggaaac tgacttataa tttaaacatc acaaaggcat gggagggata cggctgaaga
    18061 aatgctcatg caggcatggt tgtgaaatgt ggttttgatt cgcatagagg tggttatgcc
    18121 tgatctggtt ggaaagtctt tgggacgtta tcacatcggt gaacagctgg gcgaaggcgg
    18181 catggccatc gtctataaag cctacgatac ccgcctcgaa actgatgtgg ctgtcaaagt
    18241 catccgcaca gataatattc tgcccagcgt gctggatagt gccctcaaac gttttgaacg
    18301 tgaagccaag ttgctggcac gtctgctcca cccgaatatc gtcaaggtca ttgattatgg
    18361 ggaatttgaa ggtatgccat atctggtaat gccttattta cccggcggca ccctgaaaag
    18421 aaaattgggc aagcccacct cctggcagga atcgttcaaa ttgctgctgc cgatcgccga
    18481 agctttggat tatgcccaca gtcaaaacat gatccatcgt gatgtgaagc cgtccaacat
    18541 cctgctcacc caacgcggac agcccatgat ggccgatttc ggcattgcca agatcctcga
    18601 cctcgaaaat acacaggagt tgacaggcac aagcatgatg atcggtacgc cggaatacat
    18661 ggcccccgaa caggtcaccg ccaaaaccgt tgaccatcgt gccgatatct atgctcttgg
    18721 tgtcgtcctc tatgaaatgg tgacgggccg tcaacctttt ttggcagata ccccgatggc
    18781 cgtgctgctc aaacacaata gcgagcccct tccgcacccg cgccagtttg tgccgaattt
    18841 acctgacgca gtggaaaagg tgttgctcaa agcactggca aaacatcccg atgaccgcta
    18901 tcaaagcatg gaggaatttg gcgctgtgat gaagcggctg cttcaagagc cacctccgca
    18961 gttcgaggca ggatcaacct ggggtacatc cagaacgcag acagtccaat ccgccaacgg
    19021 aaattcaggt tggaaaatct tgaagtgggg agttgttgca ggcttgtttt gtcttgttgc
    19081 aggagcaggt ctcttttttg tgtttcaacg tttgaagaat ctgcctgtga actctgccat
    19141 ggtttcaaca cctgtggcgg cgactgagac gccgtctccc gttgtgccgc ctactgaaac
    19201 acctacaagt gcgccgaccc cattgcccgc ccgcattgaa gatgaaaagg gtgttcccat
    19261 ggtattgatc ccgtcagggc cgtttttgat ggggtcgttg caggaacggg tggaacagga
    19321 ctttgcaacc tgcgagaagg agaccaacgg ccagtgtgaa ttatcgtggt tcacaaatga
    19381 gacgcctcaa catttggtgc ggctcgatga ttattacatt gacatgtacg aggtgacaaa
    19441 tgcgcgctac gccgagtgtg tagccgcgga tgtctgcgtt gcgccatcgc cgaccagttc
    19501 atttacaagg gatgaatatt acggcctgcc ggaatttgcg gattatcctg tgatccatgt
    19561 cagttgggcg gatggcaggc tttattgtga ttggcgcggc ggccgcctgc ccaccgaagc
    19621 agaatgggaa aaagccgcac gcggcccgga tggtttgatc tacccgtggg gcaatgaatt
    19681 cgacggtacc aaggtgaatt tctgcgattc caattgtccg atcgattggg caaacaagga
    19741 ttacaacgac tactttgggg atacctcacc tgttggctat tacgaaagcg gcaaaagccc
    19801 atatggtgtg tacgatatgg cgggcaacgt ttgggaatgg atcagtgact ggtatcagga
    19861 ggactattac agcatctctc catcgttcaa tcctctgggc ccggaaacgg gtcagttccg
    19921 tgccttgcgc ggcggttcat ggagcaacgt atccagtgcc atgccctcct ccatgcgcag
    19981 ttgggatgtg gtgccctcgc tggcccaatt cggttattat ggctttcgct gtgcccgtaa
    20041 cgcgaacgag taaatcgggt attaaattta tcaggccgca ttttacaatc cagtcactac
    20101 tttattgacc ttaacttccc tcccatgtcc caaactccac gaccccttaa ggtatttctt
    20161 tgtcattctc attcagaccg cactgccgta cgtgctttgt attcccgcct ggttttagac
    20221 ggcgtggatg catggcttga taaggaaaaa cttctgcctg ggcaggagtg ggaattggaa
    20281 attcgcaggg cggtgcggga atctgatgta gtggtgatct gcctttcaaa tcaattcaat
    20341 caggcggggt tccgtcaaaa agaagtgcgg cttgcactgg ataccgccat ggaaaaaccc
    20401 gaaggggaga tctttatcat ccctgcccgt ttggaggaat gcgagagtct ggcaagcctc
    20461 agtaaatggc aatgggtgga tctgttcgaa gagaatggtt atgaaaaact tttgcgcgcc
    20521 cttgtccttc gtagtgatcg catccgggcc aatgcgcgtt ccctgatgcg gttcgatgcc
    20581 aaagcggaaa agaggaggca ggtcgttttc ctggtccagc aagcggagaa gatggaaaag
    20641 gacgccgatt ggatggcggc catagagaat tacaggcagg tgaatcgcat tgaccctgcc
    20701 tattttggtg tagaagagaa gatcgcatcc cttcaacaaa aaatgcaggc aggcactgtc
    20761 acggttacac tccgtcagga tgcagttgtt gacaagcctt cctcatcgaa atggctgcgg
    20821 gttgcaggta ttgtcggggt attgcttctt tgttgtatgt ttgtagtggc tggtgggata
    20881 ctcttgtatt cccgttctcg aaaaagctct gcacagatag ctccaagccc tgtcgttgca
    20941 actgcatccc cttctttgcc agaaccctct cccacacccg gtcaacctgc tgttgtgagt
    21001 acgcaacctg tcacccctga gatggtgctg attcctgcag gtgatttcaa tatgggcagt
    21061 gaaggcggtg atgcagatga acttcccatc cacttggtga gattggatgc ctattctatt
    21121 gatgtatatg aagtaaccaa tgctgcctat caggcatgtg tggactcttc agcctgcaaa
    21181 ccacccatct ccaccgggtc acttacaagg gaagagtact atggtgtctc tgagtatgag
    21241 aatttcccag tgattaatat agattgggat agggccagta ctttctgcgc ctggcgcggc
    21301 ggccgcctgc ccactgaagc agaatgggaa aaagccgcac gtggcaccga taatcgcatt
    21361 tatccctggg gtgatgcgcc cgactgtgca aaaagcaatt gcaccgctga cacattggaa
    21421 gtcggcagtt ttggagaggg aaaaagcccc tatggtgtgt acaatatggc gggaaatgtc
    21481 tgggagtggg tggccgattg gtattcgcgg gaatactatt ccatttcccc gcttgaaaac
    21541 ccgcagggcc cttcgagtgg cgaatctcat gtcttgcgtg gtggtgcatt tgatgccact
    21601 gaacgcctta aacgggtctc ggcacgcggg gcatattttc ccgaagattt ttttagcagg
    21661 gtcgggttcc gctgcgcggc ggatgcgccc tagtgcgggg ggaatgacgt ataatttcgc
    21721 ggtggctcat tctttaatgt gatcggatgt gacagacgga atggaaaatt ttatcggaaa
    21781 acacattgac cgttacaata ttatcgacct gttgggacag ggcggcatgg ccaccgtgta
    21841 tagagcctac gacacccgcc tcgagcgtga agtggcgctc aaggtcattc ggcatgaagc
    21901 ctttccgccc gacgtattgc agaatgaact caaacggttc gaacgtgagg cgaaatctct
    21961 ggcgcggctt tcacacccga acattgtcaa ggtgttggac tatggggaat atgaaggttc
    22021 gcccttcctt gtaatggaat atctcccggg cggcacgctc aaacaaaaga tcggttcacc
    22081 cattgcgtgg caggatgcca tgcatttact gttgcctgtt gcgcgcggag ttgaatacgc
    22141 ccacaaccgc gagattattc accgcgatat caaaccctcg aacattctgc ttgccgaaaa
    22201 tgaaacaccc accctgtctg attttgggat cgccaagtta ttccaaaacg agggcacgct
    22261 gctcaccgct tcgggcatgg ccatgggaac acccgaatac atggcgcccg aacaatggac
    22321 gggaaagacc gtgccgcaat cggatatgta tgccctggga gtcgttctgt acgaaatggt
    22381 caccgggcgc aggccctaca cagccgatac acctggcggc gtttttatga tgcaagtgac
    22441 cgaacctgtg ctttttccgc gtgatattgt gccgcacctg cctgaagcgg tggagcagtt
    22501 tttgctcaag gctttgtctc gcgaaccttc caaccgccat gccagcatga ccgaattcat
    22561 tagagacatg gaactgttgt tgagcaatgc ggaaacgaag gtacccatta acaggatgga
    22621 gactctcaaa ggatcgccgg gccagtcgtc ggttaggaaa gacgcgtctt ctccgagccg
    22681 aaaactggtc ccgtttaatt ggaaggccgc cactttgggt ggagtcatcc tgttggccgt
    22741 cgtcgtcgca gcggcagtag tgttgatgaa gaagaaatcc accccattgg cagcggagat
    22801 caccgattct aaaaatgcgg tgatggtgct tgtgcctgaa ggtgattttg taatgggcag
    22861 tgataacggt gatgaagatg agcggcccgt ccacacggtg tatctggatg aattctatat
    22921 tgataaatat gaggtcacca attcagatta cagggtctgt gtggtcgatg gggtctgcaa
    22981 acctccacag gatatgacga actacaacaa ccccgagtat gagaatcatc ccgtggtgta
    23041 tgtggattgg gagatggcga ggacctattg cgattggcgc gatgcgcaac tgcccaccga
    23101 ggcgcaatgg gaaaaagccg cgcgcggaat ggatgcacgt acctatccgt ggggtgaggg
    23161 gatcgcttgc agtcaggcga attacctgag ttgtattgaa gatacccagc ctttcgattc
    23221 ctatgcgggc agcctcagcc cgtacggcgc ctataacatg gcgggcaatg tttgggagtg
    23281 ggtggccgat tggtattcgc ccgcatacga tgtttcagag acagataacc cggttggggc
    23341 cgagagcggg gagcgccgtg tgctgcgcgg cggcggttgg aatcaacacg aatatttgct
    23401 tcgcaccagc gcccgtcttg gggttgtccc caccgatgtc tttttgagtt ttggctttcg
    23461 ctgtgcaagc gatgtgaatc aataaaaatt acaagtgaga attttcatgg atgcgtcttc
    23521 ccccgttcaa cccaaccctc aaaaaagatc gaaccgcccg atcgctgccc tgctgctttc
    23581 gctttcctcg tttttcttct gctgcatctc cgttctggtc aacaacccga atgggttcga
    23641 tgcttttatg gaaggcaaac ccagctggta cattgcgcag gcgttgatgt gccttgccgc
    23701 actcctgccc ctgatcggcg ccattttggg aatcctttca ctgcgtgcag gtgagatcaa
    23761 tcgcaatttg gcgattgccg ccatcgtgct tggagtgatc ggcttctttg aatctctggg
    23821 catttttgga tacgtgatgg tgttcgtcgg catcatcaat gtgttttcac cgccgtaaag
    23881 gtggatgttg tcagatgcaa acagccagtc tgcagtggac tggctgtttt tcttatgtgc
    23941 ttgtaatttt ttaagtgtta cttttcctca agcattattt tcatgactgc caataaagca
    24001 gggacttcat tttccactat gtcccaaact gtttcatcat tgatgccgaa atattcatgc
    24061 gccacaatgt cacggaagcc agcgattttt cgccatttca catcggggta tttctgccgt
    24121 gttgcctcgg aaatattctt cactgcttcc ccgatgattt ccagattgcg taacactgcg
    24181 tcgaaatgaa ggtcgtcgtt gacaaagtct ttataggtca tccccttggt gaatctcaga
    24241 accttttcac aagattcttg aacatctgcc agataaagtg attcatcccg tgacatggac
    24301 taggtcctgc ataatatagg gtttcatacg tggcttgatc gccttcgggg tgaccagatc
    24361 cactttgcag ccgagcaagt cttccagata gaacttcgtg tccatgtaac ggtcaagtgt
    24421 cgctttcccc ttgaattcca caaggatgtc cacgtcgctg tttggacctt cctctccgcg
    24481 tgccaccgac ccaaataagg acaatgactt tacgcccagt tttgtcaatt gggcgcggtg
    24541 cttgcggata cgggtaatca ctgtcctgcg tgtcatattc aaattataca acaggaaaaa
    24601 cagccag
//
LOCUS       DAOQLC010000066        24277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_692_length_24277_cov_25.868467, whole genome shotgun sequence.
ACCESSION   DAOQLC010000066 DAOQLC010000000
VERSION     DAOQLC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 24277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_692_length_24277_cov_25.868467"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PL000_12560"
     CDS             <1..1587
                     /locus_tag="PL000_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89779.1"
                     /translation="KNWLLLLLSALFGVWALAFIYHSSYVASDGRLYFNLFDDAMISM
                     RYGWNLAHGHGLVWNAGGAERVEGYTNLLMTLVMSVATFLFDKRYAVFAIQLTGIFFT
                     LGTAWMTVCIGEGISKNAESNFGTSDSGLHRYLPFALILLYYPLNFWSLMGMETGMLA
                     FFLTAGIFLSLKYADSQTSADSQILYMSVLFGLAFLTRNDSLVFAALAFLFLLPTLKR
                     GRRHVYLFLAAGALYSAFVLGQMAFRYFYYGEIMPNTYTLKLVGMALDERLRNGWGFI
                     LPFLKETAALLLVALAGLALRFSWKRLYLFGFFLVSVMYQIYVGGDAWNYWRIMAPTM
                     PLLLLLFSFACFEIAERFARTGWIRNFTLAALSVFGLFFADARFMKEFLLLDLPYQNN
                     YAQAHVEVAIAINDLTKEGATIGVFWGGTLPYYTDRTAIDFLGKSDKYIASLPPDISG
                     QSAGFGMNSMPGHNKYDLTYSIQQLLPTYVEEFDWGTQKLSGWAEEYYVRVKYNDAKL
                     YLLKGSPDIDWDKVTTILAW"
     assembly_gap    1588..1687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1688..>3208
                     /locus_tag="PL000_12565"
     CDS             <1688..>3208
                     /locus_tag="PL000_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89780.1"
                     /translation="AFIYHSSYVAIDDRRYFNLFDDAMISMRYAWNFSHGHGLVWNTG
                     GAERVEGYTNLLMTLMMSVATFLFEKRYAVLAVQLTGIFFVLGSAFLALQIFHTINPN
                     RQPAAKYFLFTAILLYYPLNYWSLMGMETGMLAFLLGAGVFFSLQYANSQPLTESLKR
                     RLPPIFPMSVLFGLAFLTRNDSLLFAALAFLFLIPTLKNGHRSISIFFAAGFLYSAFV
                     IGQTVFRYFYYGEIVPNTYTLKLVGMPLVDRLQNGWGFIQPFLKETSFIFLLALIGVL
                     LKFSFQKLYLLAIFLTSVLYQIYVGGDPWNYWRIMAPTMPFLFILFFEGCAEALRFFP
                     KLKTAPASSIIIFTLALCGLSLADSRFLREMTLSALPHDNGATHKQTDIAIAINELTD
                     EKATIGVFWAGTIPYYTDRPAIDFLGKSDPYIANLPPDLSGQMGWLGMSSVPGHNKYD
                     LNYSIKILMPTYVQEFEVGSQNLNDWGAEHYVNVKYHGIRLYLLKDSAYVDWEKAR"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3309..3860
                     /locus_tag="PL000_12570"
     CDS             <3309..3860
                     /locus_tag="PL000_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89781.1"
                     /translation="LHNFDGDVTVAPSPNLPRYEEVIEIIGNKRPDLFNPREYDELKS
                     GSFFLLAGLAFLVLFLSMIAGVGYFLYSSPDTARLGSVIFAPLIVLFIVMVVFAGTIV
                     FRPRSLTLDGKSMTIKYLFREKTLLADEIRSVQFAYQQSRNGKIFFVLLHLVNRKSLR
                     LSGLNPNLPVVYLVLKNWHQQNG"
     gene            4162..5790
                     /locus_tag="PL000_12575"
     CDS             4162..5790
                     /locus_tag="PL000_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HNC89782.1"
                     /translation="MTQHRITDDLDALLDVLPTSIRHAVEKANNSDKLLEIVIDLGRV
                     PTARFVEGEIVLSEKEITRAEIDHITERIGTFDADNRAGMERTLHRISAIRNRRSAIV
                     GLTCRVGRAVYGTVDIIQDIIESGKSVLILGRPGVGKTTLLREAARILAENKRVVIVD
                     TSNEIGGDGDVPHPAVGKARRMQVREPMLQHEVMIEAVENHNPEVIVIDEIGRELEAL
                     AARTIAERGVQLIGTAHGQTLDNLLLNPTLSDLVGGIEAVTLSDEEARRRGTQKTVLE
                     RRAPPTFDVLIEIQHRERFAVHLDIMSAVDSLLRDVPMPPEIRTRDEAGQIQIEKAAP
                     TKPAQKADTKLPRRGRGTTDQTTGRPAPVIQEQTQRSDSINPPVIGTPSVGARLQTVR
                     VFAYGVARNRLMQAAKRIGVPAVVVADVSEADVLVTLRTYYRNREKTVIDAESRGMPI
                     YVLRANTVAQVEQFLGDLYNLTEHQAPRDNMEDIRGQTEQAISAVLNGERWVDLPPGP
                     SLVRRLQHEMARNAELVSHSYGKEPRRHVRIFRE"
     gene            5897..6517
                     /gene="tmk"
                     /locus_tag="PL000_12580"
     CDS             5897..6517
                     /gene="tmk"
                     /locus_tag="PL000_12580"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNC89783.1"
                     /translation="MFITLEGPEGSGKTSHIPYLVEYLREQGYVVFPTREPGGTSISE
                     QIRDILHDLKNAEMHPRTETLLYQAARAQIVEQVIQPRLSAGEIVISDRYFDSTIAYQ
                     GYGHQQNLDEVRALVKYATGGLTPDLTVLLDLDVEVGLARKKGDKDWNRMDAKTVEFH
                     KRVRAGYLEMVKAEPKRWIVVNSDQSWETVQEGLKRVIVGRLQGEG"
     gene            6523..7209
                     /locus_tag="PL000_12585"
     CDS             6523..7209
                     /locus_tag="PL000_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HNC89784.1"
                     /translation="MPLNLHLLSTPGRGDISDVIEASRPYLEDKRDPLVAYLPLGSLY
                     AERWLELNETAFKGLARLQMVNTELMRQKEIEDILRDASLVYIPGGNTFLLNHRLHLN
                     GIALYLKKKIQAGLPLVGFSAGAILCGPNILTSKDLNTVGTTFFDGLGAVPFNISPHY
                     PLDLHGQSVKDEWLADYHFFHDNPIVMLSDGAHIKVDGKKATLVSGEAWIWRKDEDKE
                     QLEEGKLIVG"
     gene            7219..7725
                     /locus_tag="PL000_12590"
     CDS             7219..7725
                     /locus_tag="PL000_12590"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNC89785.1"
                     /translation="MDEQWMPGSKKELMSAIEREWKLLLQLAENLTDAQMTRPDPGGW
                     TPKDNLAHLTEWMNVLIGVHLDKRPAHEVLGVAANVTESWDMELINPVLFERNKDRSR
                     QDVLDGLKKTYGMVYARLDAMSFEDLLAPRHATDPEKRPILLWVLGDTTEHFLEHRET
                     IERGLKAG"
     gene            7728..8474
                     /gene="map"
                     /locus_tag="PL000_12595"
     CDS             7728..8474
                     /gene="map"
                     /locus_tag="PL000_12595"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNC89786.1"
                     /translation="MIVESEKDINSLKAIGRVVAQALKLMMEQARPGMTTAELDAIGG
                     AFLEKEGARSAPQVMYSFPGANCISVSPVIAHGIPGDHVLREGELINIDVSAEMGGYF
                     ADTGGSMVIAKHVPVYEKMIEATKSALVKAVQAAKAGQPLNGIGKAIQDEARRNGFGV
                     IFDLTGHGIGHKLHEEPTQVYNYFKSKDRRVLEEGVVLAIEPFLTTGKGHVLEEADGW
                     SLRTIDRTIAAQFEHTVIVTKDEPIILTLA"
     assembly_gap    8501..8600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8911..9492)
                     /locus_tag="PL000_12600"
     CDS             complement(8911..9492)
                     /locus_tag="PL000_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HNC89787.1"
                     /translation="MKSICVFCGSSDSVHADYKTAAFQMGSTLARRGIRLIYGGGKTG
                     LMGEVANGALSAGGEVIGIIIPSMNTPALAHTSLTRLDVAPDMHGRKARMHELSDGYI
                     ALPGGFGTWDELFETITWAQTGAHEKPVGLLNVKKYYAPLLAAMDHAVQEGFVFEEHR
                     KAVFCEMTPETLLDEMSKYQHPRDAVKRWLKED"
     gene            9591..10487
                     /locus_tag="PL000_12605"
     CDS             9591..10487
                     /locus_tag="PL000_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNC89788.1"
                     /translation="MEYKDYYKTLGVDRKASVDDIRKAYRKLAMQYHPDKNPGDKKAE
                     DKFKEINEAYQVLSDEQKRARYDQLGSAYSNFRTSGGRPGDFRWDDWFQQQQGTQQRG
                     YGTEDIFGGAGGFSDFFRTIFGEAMRSSGREQSAEQGYQQEAEITFKEAYNGTLRQIQ
                     TNGRTLQVRIPAGVKTGSKVRVAGAGPDGLDLYLVVNIIDGGQFERDGQDLYTTSSVN
                     VLTLIIGGEAEVETPTGKVMLNIPAGTQVDQVFRLAGRGMPSLKTPHTNGDLFVKLKP
                     LVPKYLSPKQRELLEEAARIKF"
     gene            10509..11642
                     /locus_tag="PL000_12610"
     CDS             10509..11642
                     /locus_tag="PL000_12610"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC89789.1"
                     /translation="MKTKRSIAAFVVLVLAMLACQMPALPVQQAASPSEPVLPVSPLN
                     SDTANPVTDADGLVALFENVSPGTVAIITDVGQGSGFVYDGNGHIITNYHVVEGASTI
                     EVRFTSGYMAYGTLIGTDLDSDIVVIKVDAPAGELHPLPLGDSDSLKVGQTVVAIGNP
                     FGLDSTMTVGIVSALGRTLDAIRETPEGRPFTAGDIIQTDAAINPGNSGGPLFNINGE
                     VIGINRAIRTTSTTETGQPVNSGIGFAISINMVKRVVPVLIETGQYDYPFLGISSLDS
                     LSLDMINELGLKSFTGAYITDVVSGGPADKAGLIAGDTPTAIPDLFSGGDLIIAIDGR
                     DIRTFDEMLAYLITHKSPGETVILTVLRGEEKVDVSVELGKRP"
     gene            11760..13487
                     /locus_tag="PL000_12615"
     CDS             11760..13487
                     /locus_tag="PL000_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89790.1"
                     /translation="MKTFRVPLIRKILSLLALLMVLSLTGFVTYLCVQEFDVFTRILE
                     GRRSGFFVLFGLLVLVALTIKFVVYFMAVLNAQLIFDDKRMTIEAISGPGFLLWRGLK
                     PFSIELERVRSVFFNLLGMLEIIDAQGKRYAFSPGLFSMGSGEDILLELKSRVPPRAL
                     APILENSENFKHTHNWVFIRSLAFPVILMLYLLTMYLEPPNSELIHVWKSEMRLSWLE
                     DVEGYSVDPSGGFWVLSDTFAGYRVYRFLSSPTQSWKWNLEPFETQYPKFVSGDAEGT
                     PVIWLENGMMRYVNKKWEIMPYKENLVLESDRMFEAITEGHVLVLNGNAGQLLNINVS
                     NGEWYALTLPQDARQNRFVPASIKRTVDGEILVLMKNDRESRVYLLSNGEWSSQEYAI
                     IQADGRVRDYFLDEDDMLWVLLSIRGQSIVERLDNEGNVSVTKLPSMSNKVDAVRYSS
                     LHVDSSGRLWVEGKYPRFISVFAPVWGGDVDEIIRYSVENSNYQSGISSSTVLMPDGK
                     LWAFEKQVVSMDTNLNDLPAPLPDWFESLDLGVVRLILMMIFNILLITPSVVPMIYSL
                     SFPVRKQKL"
     gene            complement(13537..14178)
                     /locus_tag="PL000_12620"
     CDS             complement(13537..14178)
                     /locus_tag="PL000_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HNC89791.1"
                     /translation="MFAFEGFDRRKEEDRVRAEGRLPPGQSLTQKFPVLHYGPVPAFD
                     PATWDFRVWGEVEEEKRWSWDEFNKLPRSRIDMDIHCVTRWSKFDTTWEGVSVKTLVE
                     QGFFKIKPNATHVMQHAEYGFTVNLPLEVVLQENFLLATHFMGEPITPDHGYPLRGVV
                     GFIPGREDMETPYFWKGAKWLRSIEFMPRDKRGFWEQAGYHNRADVWREERFG"
     gene            14373..16280
                     /locus_tag="PL000_12625"
     CDS             14373..16280
                     /locus_tag="PL000_12625"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC89792.1"
                     /translation="MKAPTFKQRFQYWFDNYMSQGTGALIGGLAVVSVVIISIAALII
                     AIGGRFLAPEGAEPPGFVEAFWLSLMRTLDAGTMGGDAGWGFRLMMLLVTIGGIFIVS
                     TLIGVLSNGLEDRLEELRKGRSVVLESDHTLILGWTPQIFTVISELVLANESRKSGAV
                     IVVLADQDKVEMEDAIAERIPDTKNTRVICRSGNPIDLTDLEIASPHTARSIIILAEG
                     ADPDTHVIKSVLAITNNPNRREEPYHIVTQIRDPKNMDVVKMLGVKDTVQPILTNDLI
                     ARVVAQTSRQSGLSIVYTELMNFGGDEIYFKQEPELSGKTYGEALEAYETSTLMGVRK
                     ADGTIAMNPSMDSRIESGDQIFVIAEDDDKIKLTGIGRKQFEERLIQVSGAASRSKPE
                     KTLILGWNRSGATIIRELDNYVAKGSQVTIVSDIYELDKQIKLDSGALKNQKITVKEG
                     DIRDRELLESLEATEYDHVIVLAYNHLEQQEADAITLITLLHLRDIAERDSTPFSIIS
                     EMLDLRNRELAEAAKVDDFIVSEHLISLMMTQLSENSELLGVFTDMFDPEGAEIYLKP
                     ISDYVVTGEPVNFYTVTEAARRRGETAIGYRVMSESHDASKAYGVHTNPKKSERVSYS
                     PDDKVIVIAEG"
     gene            complement(16320..17561)
                     /locus_tag="PL000_12630"
     CDS             complement(16320..17561)
                     /locus_tag="PL000_12630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HNC89793.1"
                     /translation="MTKPVYDILLEKMTYGGEAMGRLPDGRALFVPFGLPGEKVRVDL
                     VEEKKGFARGALLEILQPSPERIAPKCKHFGVCGGCHYQHLPYEKQLAAKTEILRDQL
                     QRIGKIENPPVRPMIPSPLEWNYRNHVQFHLTAEGRLGFIHAKGNSTLPIEECHLPEA
                     GIHAFWPDLQFESNEGVERVSLRSGQDGELMVVFESDTPETPELEIEADVSVVHLFED
                     HPVVIAGEDHLTIKVSNRDFRVSAPSFFQVNTKMAEAMVQHLVSKLPVNETTTLLDVY
                     CGAGLFSAFFAQKFKRVVGIETSASACEDFAFNLDEFENIELYEGAAEEILPALADQL
                     KDAACAIVDPPRAGIEKHALDALIAIKPQVIAYVSCDPSTLARDAARLIHGGYQLVEV
                     TPFDLFPQTYHIESISIFEKN"
     gene            complement(17646..17816)
                     /locus_tag="PL000_12635"
     CDS             complement(17646..17816)
                     /locus_tag="PL000_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89794.1"
                     /translation="MAPKNKRPARVSPAAPQQTAAEFNPDYTQVKKDLTRIGLLSGTF
                     FILLVALSFVIK"
     gene            complement(17829..18740)
                     /locus_tag="PL000_12640"
     CDS             complement(17829..18740)
                     /locus_tag="PL000_12640"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HNC89795.1"
                     /translation="MARKKAPEDLSVEELRRLLVEKRRGARKDRLEYFRRTGRVLSIS
                     PGPSEAESDRAAPIVDTAEAAESSPEQVPVNPRRKFMDRVLLGVEVLAVLGLIAVLLN
                     SFGMLRTLNTEVASALNPETVTPTPLVMAVVLPSGHTPPDAQGNTRPNEAEIPDHLRP
                     MVQSLVNLPVPTAAPDQAVRLQIPALNIDAPIVQGDGWEQLKKGVGQNIGSSNPGQDG
                     NVVLSAHNDVYGEIFRYLDRLAPGDQVIVYTQQRQYTYIVDRTILVEPTAVEVMASTG
                     TPTVTLISCYPYLVNKQRIVVFARLQN"
     gene            18803..18876
                     /locus_tag="PL000_12645"
     tRNA            18803..18876
                     /locus_tag="PL000_12645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18837..18839,aa:Val,seq:gac)
     misc_binding    18956..19190
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19352..21160
                     /gene="thrS"
                     /locus_tag="PL000_12650"
     CDS             19352..21160
                     /gene="thrS"
                     /locus_tag="PL000_12650"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNC89796.1"
                     /translation="MSQITERYEDSQLYRIRHSAAHVMAQAVLEMFPDGKVTIGPPVE
                     NGFYYDFDLPRNLTPEDLEAIEKRMRQIVQGKHEFRKTVISAEEAKKIFADQPYKLEL
                     IEGLEKGGLDEYGNPLAEKPDISIYQHDTFTDLCRGPHVANTKEIKQDAFKLMSIAGA
                     YWRGDENNKMLQRVYGTAWESKDQLKEYLQQLEEAKKRDHRKLGKELEIFIFDDEVGP
                     GLPLWLPNGGVLIEELENLAKEMELKAGYDRVRTPNVTKEDLFLRSGHLPYYAESMYP
                     PMELEGVKYYIKPMNCPMHHKIFGSKGRSYRELPVRLAEYGTCYRYEKSGELFGLMRV
                     RSMQMNDAHIYCSEEQFEQEFMGVVELYKKYFDLFGIEKYVMRLSLHSKAGLGKKYVN
                     NEALWIKTEDMVRRAMNNGGVPYVEREDEAAFYGPKIDVQIWSAIGREFSLATNQVDF
                     AVPERFDLKFTNKEGHDEVPLCIHRAPLSTHERMVGFLLEHYAGNFPVWLSPEQVRVI
                     SITDGQNEYVDKIARELRENGIRASADVSSQRMNAKIRQAQLMKVPYMIVVGDNEMQA
                     NQVSLRARDGSQQNNISLGEFIARAKDRISRRAKEL"
     gene            complement(21226..23469)
                     /locus_tag="PL000_12655"
     CDS             complement(21226..23469)
                     /locus_tag="PL000_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89797.1"
                     /translation="MKNNRPFNLLDDIAKDHIPDSTNLTPHLAARLERNSRMFTFRTH
                     RLVAMLIALIILIALSGVAYAIGRSLGYIPGVGIVDQSTPIRILAEPVTQTRDGITIT
                     VSGVVLSSSKTVVLYKVENIPQEKLSQDMAFPGCGSKPEEHTFLKTVEDPMPRDLGYE
                     MNGWGTGYEVRHIFAALPAEINDAVLVIPCIERSLSGTLPENWELPLHFIPAPPEMSV
                     APVIEVTPSQEPVEGSPLKLEKIAETEKGYILVGTFDSTGLPQGAQAMDFSTFPIFTD
                     ANGQEVIYDFANFQLDQPAEFPPIGTFPWAFEIIGKDHAYPLTMTVSAVAAQYSGSSA
                     SFEFDAGANPQEGQVWDLNQDVELVGFPVRVVKATRTVEGYVFNFESSAFFHGVSLTM
                     GEGSLPGGTGMDDPNHFKAVVRFNGAIPSGKMEVLITNPVMAISGNWNIQWQPEISST
                     ESVSTPTTADQTCLTRDSWQAALDNYQPVPPELNGRLLVYGALSDDWLNYDNYGSFMS
                     RLDRSDKQIISQGVFPSLSPDGKFVAFAWGEGLNIAEIASGAQFHIPNTTSDDNFPVW
                     SPNGAKIAFISGTDLNLYIINPDGSERTRVADLNGDEQLIDWTNDGTGLFYGVSVEGG
                     ISLRKIDLVSGAVAELFTIPQHRTYEDVAISPDGTHIAYHARENLQDAIYISNLDGSE
                     RRLFAQMGTWIVGAPFWSPDGNWLIVIIRSTDLPDRPTEMALVNVNDCRIHPFPLSVE
                     MVRGWMP"
     gene            complement(23472..24017)
                     /locus_tag="PL000_12660"
     CDS             complement(23472..24017)
                     /locus_tag="PL000_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC89798.1"
                     /translation="MDEVTAIRRIKMGDFGGLEYLMSLYQVKAARAAFLITCDKAKAE
                     DVVQEVFLRIFQRPGSFDENRPFEPYLMRSVVNAALNASRGEKRFISLEANAETIERL
                     ILQEASVESQAEYSQLKSEILNALGQLPPRQRAAIVQRYYLDMSEKEMAFTLDAAPGT
                     VKWLLNSARNTLRDLLKKERI"
BASE COUNT         5627 a         5952 c         6389 g         6009 t
ORIGIN      
        1 aagaattggc ttctgctcct cctttctgcg ctgtttggcg tatgggcact ggccttcatt
       61 taccattcct cctacgtggc cagcgacgga cgcctgtatt tcaacctttt tgatgatgcc
      121 atgatctcca tgcgctacgg gtggaacctt gcacacggac acggcctggt gtggaacgcg
      181 ggcggtgcgg agcgtgtcga aggctatacc aacctgttga tgacgctggt catgtcggtg
      241 gctacatttt tgtttgacaa acgctatgcc gtgtttgcca ttcaattgac gggcattttc
      301 ttcacactcg gcacagcatg gatgacggtc tgcatcgggg aggggatttc aaagaacgcc
      361 gaatcaaatt tcgggacgtc tgattcgggt cttcatcgtt atctcccttt tgccttgatc
      421 ctgctctact atccgctcaa cttctggtcg ctgatgggta tggaaacggg gatgctggcg
      481 ttctttctca ctgcggggat tttcctctcg ctcaagtacg cggactcgca aacctccgct
      541 gattcgcaaa ttctttacat gtccgttttg tttggcctgg cctttctcac ccgcaatgac
      601 tcgctggtgt ttgcagcgct ggcgttttta ttcctgctcc caaccttgaa gcgcggacgc
      661 cgccatgtgt atctttttct tgcagcaggg gctttgtatt cggcttttgt ccttgggcag
      721 atggcgttcc gttattttta ttatggcgag atcatgccaa acacctacac gttgaaactt
      781 gtcggcatgg cgcttgatga acgcctgcgg aatggatggg gattcatttt gccgtttttg
      841 aaggaaacgg cggcgctttt gctcgttgcg cttgcagggc tggcgctgag attttcgtgg
      901 aagaggctgt acctgtttgg ctttttcctt gtttcggtga tgtatcaaat ttacgtcggc
      961 ggcgatgcgt ggaattactg gcgcatcatg gcgccgacca tgcctctgct gcttttgctc
     1021 ttttcattcg catgttttga aattgcagaa cggtttgctc gaacgggttg gattcgcaat
     1081 ttcacactgg cggcattgtc tgtttttgga ttgttttttg cagatgctcg cttcatgaag
     1141 gaatttttgc tgcttgacct gccataccag aataattatg cacaggccca tgtggaagtt
     1201 gcgattgcca tcaatgacct gaccaaagaa ggcgccacca ttggggtctt ttggggcggt
     1261 acgcttccct attacaccga ccgcaccgcg atcgattttc tcggcaagtc tgataaatac
     1321 attgccagcc tgccgcctga tatttcaggg cagagcgcgg gcttcggcat gaacagcatg
     1381 cccggccata acaaatatga cctgacctat tccatccagc aactgctccc cacctatgtg
     1441 gaagagttcg attggggtac gcaaaaactc agcggctggg ccgaggaata ctatgtgcgg
     1501 gtgaaataca acgacgccaa attgtatctt ctgaaaggtt cacccgatat tgattgggat
     1561 aaagtgacca ccatccttgc ctggtgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnntgg catttattta tcattcctcc tacgtagcca tagatgaccg ccgatacttc
     1741 aacctcttcg acgatgcaat gatctccatg cgttacgcat ggaatttttc acacggacat
     1801 gggctggtgt ggaatacagg cggcgcggag cgtgtggaag gctacaccaa cctgctgatg
     1861 accctgatga tgtcggtggc gacctttctc tttgaaaaac gatatgctgt tttggcggtt
     1921 caactgacgg gcattttctt cgtcctcggt tcggcgtttt tggcattgca gatctttcac
     1981 accatcaacc caaacagaca acccgccgca aaatattttc tctttaccgc aattcttttg
     2041 tattatccgc tcaactactg gtcgctcatg gggatggaaa caggcatgct ggcattcctc
     2101 cttggcgcag gtgtcttctt ctcccttcaa tatgccaact cacagccact caccgaatcg
     2161 ctgaagcgcc gattgccgcc gattttcccc atgtcagttt tgttcggcct ggcatttctc
     2221 acccgcaacg actcgctcct ctttgccgcg cttgcctttt tatttttgat ccccaccctc
     2281 aaaaacgggc acagatcgat ctccattttt ttcgcggctg gcttcctgta ttcagccttt
     2341 gtcatcgggc aaaccgtttt tcgctatttt tattacggcg agatcgttcc caacacctac
     2401 accctcaaac tcgtcggcat gcctttggtt gatcgcctgc aaaatggctg gggcttcatt
     2461 caaccattcc tgaaagagac cagcttcatt tttcttcttg cgctcatcgg ggtgctgctg
     2521 aaattctctt tccaaaagct gtacctgctt gcgattttcc ttacctcggt gctttatcag
     2581 atctatgtcg gcggcgaccc gtggaattac tggcgcatca tggcgccgac catgccgttt
     2641 ttattcattc tctttttcga aggatgcgct gaagcgctgc gcttcttccc caaactgaaa
     2701 actgctccag cgtcctccat catcatcttc accctggcat tgtgcggact ttccctcgca
     2761 gactcccgct tcctgcgcga gatgaccctt tctgccctgc ctcacgacaa tggcgccacc
     2821 cacaaacaaa ccgacattgc cattgccatc aacgaattga ccgatgaaaa agccaccatc
     2881 ggagtctttt gggcggggac gattccctac tacactgacc gtcccgccat tgatttcctc
     2941 ggcaaatcag acccatatat tgccaacctg ccccccgacc tttccggtca aatgggctgg
     3001 ctcggcatga gcagcgtccc cggccataac aaatatgacc tgaactactc catcaagatc
     3061 ctcatgccca cctatgtgca ggaattcgaa gtcggttcac agaacctcaa cgactggggt
     3121 gccgagcatt atgtcaacgt aaaatatcat ggcatccgcc tctacctttt gaaagattct
     3181 gcgtatgtag attgggaaaa agcccgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnntt acacaacttc gatggcgatg tgaccgttgc gcccagtccc aacctgcccc
     3361 gctatgaaga agtgatcgag atcatcggaa acaaacgccc cgatttgttc aacccacgcg
     3421 agtatgacga attaaagagt ggaagttttt tcctgttggc aggccttgcc ttcctggttt
     3481 tatttctcag catgattgca ggggttgggt atttcctgta cagttcgccc gatacggccc
     3541 gtttaggctc ggttatcttt gcacctctga tcgttttatt tattgtcatg gttgtatttg
     3601 caggcaccat tgttttccgt ccccgctcgc tcactttgga tggaaaatcc atgaccatca
     3661 aatatctttt ccgtgaaaag accctgcttg cagatgagat tcgcagcgtc cagtttgctt
     3721 atcaacaatc acgtaacgga aaaatatttt tcgttcttct ccatctggta aatcgaaaat
     3781 cgctccgcct ctcgggcttg aaccccaatc tgcctgtggt gtaccttgtg ttgaagaatt
     3841 ggcaccagca aaacggctaa attcgtcaga caaatcaacg aaattaacac atgcctttaa
     3901 tttaatgtta atcgccatcc tgctataata tccctgcacg cgacaccaat ttaatgccgt
     3961 ggtaatgggc acccccggca cgtgaaccgt gacacccttt aataggcggt actccaacat
     4021 gctcaaccac accctcctct aacccagctt ccctgcaacg tgtttcgcgc ggatttgcgc
     4081 ggcatacctt atttcgtctt ccctcccttt taccatcacg tgtcacttga tacctgacac
     4141 ttgcaagctg aaacttaaca tatgactcaa cacagaatca cagacgacct cgatgctctc
     4201 ctcgatgtcc ttcccacttc cattcgtcat gcagtggaga aggccaacaa tagtgacaaa
     4261 cttctggaga tcgttattga ccttggcaga gtgcccactg cgcgctttgt cgaaggtgaa
     4321 attgtcctct ctgaaaaaga gatcacccgc gctgagatcg accatatcac cgagcgcatc
     4381 ggcacatttg atgccgacaa ccgcgcaggc atggaacgca cgctccatcg catctctgcc
     4441 attcgcaaca ggcgcagtgc cattgtcggc ctgacctgcc gtgtgggccg cgccgtgtac
     4501 ggcactgtgg atatcattca agacatcatc gaatcgggta aatccgtttt gatcctgggc
     4561 cgccctggcg tgggcaaaac cacgctgctg cgtgaagccg cccgcatcct cgctgaaaac
     4621 aaacgcgtgg tcatcgttga tacatccaat gagatcggcg gcgatggcga tgttccacac
     4681 cccgctgtag gcaaggcgcg ccgtatgcag gtgcgtgaac ccatgcttca gcatgaagtg
     4741 atgatcgaag cggtcgaaaa ccacaacccc gaagtcatcg tcattgacga gatcgggcgc
     4801 gaacttgaag cccttgccgc acgcaccatc gccgagcgcg gtgtacaact catcggcacg
     4861 gcacatggcc agaccctcga taaccttttg ctcaacccca cgttgagcga ccttgtcggt
     4921 ggaatcgaag cggtcacact ttcagacgaa gaagcccgtc gacgcggcac ccagaaaacg
     4981 gtcctcgaaa gacgcgcgcc cccaaccttc gatgtcctca tcgagatcca gcaccgcgaa
     5041 cgcttcgcag tccatctcga tatcatgtcg gcggtagatt cacttttgcg cgacgtgccc
     5101 atgccgcccg aaattcgcac acgcgatgag gcggggcaga tccagatcga aaaagcagcg
     5161 ccgaccaaac ccgctcaaaa agcggataca aaactcccgc gccgcggacg gggaaccacc
     5221 gatcaaacga cgggcaggcc tgctcccgtt atccaggagc agactcaacg ctctgactcg
     5281 atcaacccgc ccgtcattgg cacaccgagc gttggcgcac gattgcagac cgtgcgggta
     5341 tttgcctatg gcgtagcccg caaccgattg atgcaagccg ccaagcgaat cggcgtgccc
     5401 gccgtggtgg tggccgatgt cagcgaagcg gatgtgcttg tcaccctgcg gacgtattac
     5461 cgcaaccgcg aaaaaaccgt cattgatgcc gaatcgcgcg gcatgcccat ctatgtactg
     5521 cgtgcgaaca ctgtcgcgca ggtggaacaa ttcctcggag atctttataa ccttaccgag
     5581 caccaggccc cgcgcgataa catggaagac attcgcggcc agaccgagca ggccatctca
     5641 gccgtgttga acggtgagcg ctgggtagac ctgccgcccg ggccatcatt ggtgcgccgc
     5701 ttgcaacacg aaatggcgcg caatgccgag ctcgtcagcc actcgtacgg caaggaaccg
     5761 cgcaggcatg taaggatttt cagggaatag aacatagtaa atagggaata gggagcgggc
     5821 ttgtcctgct ccctattttt tatgttacca tttgcaacat cctattcacg taaacctatt
     5881 cactatttcc taaaccatgt tcattactct tgagggcccc gaaggctctg gcaaaacctc
     5941 ccacatccca tacctcgtgg agtatctccg cgagcaggga tatgttgtct tccccacccg
     6001 tgaaccgggc ggcacttcca tcagcgaaca gatccgcgat attttgcacg acctgaagaa
     6061 cgctgagatg catccgcgta ctgaaaccct gctctatcag gccgcccgtg cgcagatcgt
     6121 tgagcaggtc atccagccgc gtttgagcgc gggggagatc gtcatctccg accgatattt
     6181 tgactcaacg atcgcctatc aaggctatgg ccatcagcag aatctggatg aggtccgcgc
     6241 attggtgaag tatgcaacgg gcgggttgac ccccgacctg acggttctgc ttgatctgga
     6301 tgtggaagtg ggccttgccc gcaaaaaagg cgacaaagac tggaatcgca tggatgccaa
     6361 gaccgtcgaa ttccataaac gtgtccgggc tgggtatttg gaaatggtca aagccgagcc
     6421 gaaacgctgg atcgtggtca attcagatca gagttgggag accgtacagg aggggttgaa
     6481 gcgggtgatc gttggaaggt tacagggtga agggtaaata tcatgcctct taacctccat
     6541 ctcctttcca cgcctggcag gggtgacatc agcgatgtga tcgaagcgag ccgcccttat
     6601 ttggaagaca agcgtgatcc gctcgtggcc tatttgccgt tgggttcgct gtacgccgag
     6661 cgctggctgg agttgaacga aaccgccttc aaagggttgg cgcgtttgca aatggtcaac
     6721 accgagttga tgcgccaaaa agagatcgaa gatattttgc gggatgcatc gctcgtctat
     6781 attcccggcg gcaacacctt tttgcttaac catcgtctgc atcttaatgg aatagcgctt
     6841 tatctgaaga agaaaattca agcaggcctg ccattggttg gattcagcgc aggcgctatt
     6901 ttgtgcggac ccaatatttt gacctcaaaa gatctcaaca ccgtggggac gacatttttc
     6961 gatggcttgg gcgccgtgcc gttcaacatc tctccacact acccgcttga cctgcacggc
     7021 caatcagtaa aggatgaatg gctggcagat tatcacttct tccacgataa ccccattgtc
     7081 atgctttcgg atggggcgca catcaaggtg gatggaaaaa aagccaccct cgtcagtgga
     7141 gaggcatgga tctggcgcaa ggacgaagac aaagagcaac ttgaagaagg caagctgatc
     7201 gttggttagg agaccatcat ggatgaacaa tggatgcccg gtagtaaaaa ggaattaatg
     7261 tctgcgatcg aacgtgagtg gaaactgctt ctgcaactgg cagagaatct caccgatgca
     7321 cagatgaccc gtcccgaccc gggcggctgg accccaaaag ataatcttgc gcacctcacc
     7381 gagtggatga atgttcttat cggcgttcac cttgataaac gccccgcaca cgaagttttg
     7441 ggcgtggctg caaatgtgac agagagctgg gacatggaac tcatcaatcc cgtccttttt
     7501 gaacgcaaca aggatcgctc gcggcaggac gtgctggatg gtttgaagaa aacatatgga
     7561 atggtttacg cacggcttga tgcgatgagt ttcgaagacc tgctggctcc ccgccatgcc
     7621 actgaccctg agaaacgtcc cattttgttg tgggtattgg gcgatacgac ggaacatttc
     7681 cttgaacacc gcgaaaccat tgagcgcgga cttaaagcag gttaagcatg atcgttgagt
     7741 ctgaaaaaga tattaattca ctcaaagcca ttgggcgtgt tgttgcgcag gcattgaagc
     7801 tgatgatgga gcaggcccgt cccggcatga ccactgccga attggatgcc attggcggtg
     7861 cctttcttga aaaggagggc gcgcgatctg ccccacaggt gatgtattcc ttccccggtg
     7921 caaattgcat cagcgtttcg ccggtcattg cacacggcat tcccggtgac catgttttac
     7981 gcgaaggtga attgatcaat atcgatgtct ctgccgagat gggcgggtat tttgcagata
     8041 cgggcggctc gatggtcatt gcaaaacatg tgccggtgta tgaaaaaatg atcgaagcca
     8101 ccaaatctgc tttggtgaag gcggtgcagg cggcgaaagc cgggcagccg ctcaacggca
     8161 ttgggaaagc gatacaggat gaagccagac gaaacgggtt tggggtcatc tttgatctga
     8221 ccgggcatgg catcgggcat aaactgcatg aagaacccac ccaggtgtac aactacttca
     8281 agtcgaaaga tcgccgcgtg ttggaggaag gtgtggttct ggccattgaa ccctttctca
     8341 ccacaggcaa gggacatgtc ctcgaagagg cagacggctg gtcattgcgc accattgacc
     8401 gcaccattgc tgctcaattc gagcatacgg tgatcgtcac caaagacgag ccgatcattt
     8461 tgacattggc atagggagtg gagcctgtaa ataaattaca nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nnnnnnnnnn acgagccgat cattttgaca ttggcatagg gagtggagcc
     8641 tgtaaataaa ttacatgaaa catcccgaaa ttaatctttc aattctttag ccgcaaattt
     8701 atacgaaaaa agccgaacta ttttacaggg tcggcttttt tatatctcgt ccgtatcttg
     8761 tataagttgc aagaaatgtc tttgaaaagc catcttgaac cactgagttt ttaattgctt
     8821 tctctgtgtc tccgtggctc cgtggttcat ctttcagact tttaaaataa ttcgcggctt
     8881 attccttgca tttggggtga atcggttttc ttaatcctct ttcaaccaac gcttcacggc
     8941 atcgcgtgga tgctggtatt ttgacatttc atccagcagg gtctcggggg tcatttcgca
     9001 aaacacagcc ttgcgatgtt cttcaaagac aaatccctcc tgtacggcat ggtccattgc
     9061 ggccagcagg ggggcgtaat attttttcac atttaacagt ccgaccggct tttcatgggc
     9121 accggtttgc gcccatgtga tggtttcgaa gagttcatcc caggtgccaa aaccgccggg
     9181 cagggcaatg taaccgtccg aaagttcatg catgcgggct ttgcgtccat gcatgtcggg
     9241 ggctacatcc aggcgggtca ggctggtgtg ggccagtgcg ggtgtgttca tggatgggat
     9301 gatgataccg atcacctccc cgccagcaga taatgccccg tttgccactt cacccatcaa
     9361 gccggtcttg ccgccgccat aaataaggcg gattcccctc cgggcgaggg tactgcccat
     9421 ttgaaacgcg gcagtcttgt agtccgcatg aaccgagtcg gacgatccac aaaatacgca
     9481 gatagatttc attttattat tccttttgtc tcaatcataa caggttagag ttgtgttagt
     9541 gtgcgctttg ggtgcttgac gaggaaaagg cgcggtgtca caattgcggc atggaataca
     9601 aagattacta caaaaccctc ggcgtggacc gtaaagccag tgtagacgat atccgtaagg
     9661 catatcgtaa gctggccatg cagtatcacc cggataagaa cccgggcgat aaaaaggccg
     9721 aggataagtt caaagagatc aacgaagcct atcaggtatt gagcgacgag caaaagcgcg
     9781 cccgctacga ccagttgggc agcgcgtatt ccaatttccg taccagcggc ggacgcccgg
     9841 gcgatttccg ctgggatgat tggtttcagc agcagcaagg gacccagcaa aggggctatg
     9901 gcacagaaga tatctttggc ggtgcaggcg ggttctccga ttttttccgc accatttttg
     9961 gcgaggcgat gcgctcttcg gggcgggagc agtctgcaga gcaggggtat cagcaggaag
    10021 cggagatcac cttcaaagaa gcctataacg gcacattgcg tcagattcag accaatggac
    10081 gcacgttgca ggtccgcatc cctgccgggg tgaagacagg ttcaaaggtc cgtgtagcag
    10141 gtgcgggtcc agatggactc gatctgtatc tcgtagtgaa tatcattgat gggggacaat
    10201 tcgagcggga tggtcaggat ctgtacacga catcctcagt aaatgtgttg accttgatca
    10261 tcggcggtga agccgaagtt gaaaccccca caggcaaggt catgttgaac atacctgccg
    10321 gtacccaggt ggatcaggtc ttccgccttg cgggacgcgg tatgccgagc cttaagactc
    10381 cgcatacaaa tggtgacctg ttcgtaaaac tgaagccatt ggttccgaag tacctttcgc
    10441 ccaaacagag ggaattgctt gaagaagcag ctcgtatcaa gttttaaagt tgaacatagg
    10501 agtatttcat gaaaacaaag cgaagtatcg cagcttttgt tgtgttagtg ttggccatgc
    10561 ttgcctgtca gatgcctgcc ctgcctgtgc aacaggcggc cagcccgtct gagccggttc
    10621 tgccggtgtc gccgctcaat tcagatacag ccaaccctgt aaccgatgca gatggtttgg
    10681 ttgcgctttt tgaaaacgtc agcccgggca ccgtggccat catcaccgat gtggggcagg
    10741 gttcgggttt tgtgtacgat ggcaacggcc acatcattac caactatcat gttgtggaag
    10801 gtgccagcac cattgaagtg cgtttcactt ccggttacat ggcctatggc actttgatcg
    10861 gaaccgatct ggattcagac attgtggtca ttaaagtgga tgcgcctgcc ggtgaacttc
    10921 accccctgcc gctcggcgat tcagactcac tgaaagttgg tcagaccgta gtagccattg
    10981 gcaacccctt tggcctcgac agcacaatga cggtgggcat tgtctcggcc cttggccgca
    11041 ctttggatgc gatccgcgaa accccggagg ggcgtccttt cacggctggg gatatcattc
    11101 agaccgatgc ggcgatcaac ccgggcaatt cgggtggtcc gcttttcaat atcaatggtg
    11161 aagtgatcgg catcaatcgt gccatccgca ccaccagcac caccgaaaca ggacagcccg
    11221 tcaattcggg cattgggttt gcgatttcca ttaatatggt caagcgggtt gtaccggtgc
    11281 tgatcgagac cggacaatac gattatccct tccttggcat ttcatcgctc gattcgctaa
    11341 gcctcgatat gatcaatgaa ctggggttga aatccttcac cggtgcctac atcacagacg
    11401 tggtcagcgg cggcccggcg gataaggctg gcttgatcgc aggggacacc ccaaccgcca
    11461 ttcccgatct gttcagcggc ggagacctca tcatcgccat tgacgggcgc gacatccgta
    11521 cttttgatga gatgctggcc tatctcatca cccacaaaag ccccggcgaa accgtgatcc
    11581 tcaccgtttt gcgcggcgaa gaaaaagtgg atgtaagcgt ggagttgggg aagagaccgt
    11641 aggaattgat ccatgcagga gcacagcccg gaaccacagt tccgggctgt tttttttgca
    11701 accagtcggg tggagttgta taattcagac aagttcaata tttcatttgg gagggtttta
    11761 tgaaaacatt tcgtgtgccc ctgataagaa aaatattaag tctgcttgcg ttgttgatgg
    11821 tgctgtcgct gacgggattt gtgacctatc tatgtgtcca ggaattcgat gtgtttacaa
    11881 gaatcctgga aggcagacgt tctggatttt ttgttctctt cggcttgctg gttttagttg
    11941 ccttaacaat caaatttgtt gtttatttca tggccgtgtt gaatgcacag ttgatttttg
    12001 atgataaaag aatgacgatt gaagccattt ccgggccggg gtttttgctt tggagagggt
    12061 tgaaaccttt ttctatcgaa cttgaacggg ttagaagtgt attttttaat ttattgggaa
    12121 tgctggaaat cattgatgct caaggaaaaa gatatgcttt ttctccaggt ttattcagca
    12181 tggggagcgg tgaagatatc ctgttggaac taaaaagccg tgtgccaccg agagcacttg
    12241 ctcccatttt ggagaattct gaaaatttca agcataccca taattgggtt ttcattagga
    12301 gtttggcttt cccagtgatc ttaatgctgt atttgcttac tatgtatttg gagccgccaa
    12361 attcagagtt gatccatgtg tggaaatctg aaatgcggct atcatggctg gaggatgtag
    12421 aggggtattc agtggatccc tcgggtggtt tttgggtgtt atctgatacg tttgccgggt
    12481 atcgcgttta tcgttttttg agttcgccaa cgcagagttg gaaatggaat ttggaaccat
    12541 ttgaaaccca atatccaaaa tttgtcagcg gggatgctga agggacaccg gtcatctggt
    12601 tggaaaacgg aatgatgcga tatgtaaaca aaaaatggga aatcatgccc tacaaggaaa
    12661 atcttgtatt ggagtctgac agaatgtttg aagccattac agaggggcat gtgctggtgt
    12721 tgaatggaaa tgcagggcag cttttgaata ttaatgtttc aaacggagaa tggtatgccc
    12781 tgacacttcc acaggatgcg aggcagaatc gatttgtgcc agcatccata aaaagaacag
    12841 tggatggcga gattcttgtg ctgatgaaga acgatagaga aagtcgggtg tacctgcttt
    12901 caaatggtga atggagttcg caggaatacg ccattattca ggcggatggc cgcgtacgag
    12961 attatttttt ggatgaggat gatatgctgt gggtgttgtt gtccatccgt ggtcagtcca
    13021 ttgtagaaag attggataat gagggaaacg tttccgtaac aaagttacct tcgatgagta
    13081 acaaagtaga tgcagtaagg tattcttctt tacatgttga ctcatcagga cggttatggg
    13141 tcgaagggaa atatccaagg tttatttctg tatttgctcc agtatgggga ggtgatgtgg
    13201 atgaaattat tcgatactcg gtcgaaaatt caaattatca atcaggtatt tcttcctcca
    13261 cagttttgat gcctgatgga aagctttggg cttttgaaaa gcaggttgtc agcatggata
    13321 ccaatttgaa cgatttgcca gccccactcc ctgattggtt cgaatctttg gatttgggcg
    13381 tagtaagact gatcctgatg atgatattta atattttgct cattacccct tctgtggttc
    13441 ccatgattta cagtctatct ttccctgtaa gaaaacagaa gttataacgc ccgcaaaaca
    13501 aaaagggaca cggagatacc gtgtccctgc tgaaacctat ccaaaccgtt cttcgcgcca
    13561 gacatcggca cggttgtgat acccggcttg ttcccaaaat ccgcgtttgt cacggggcat
    13621 gaattcgatg gaacgcagcc atttggcgcc tttccagaaa tagggagttt ccatgtcttc
    13681 gcgcccgggg atgaatccca ccacgccgcg caatggataa ccgtggtcgg gggtgatcgg
    13741 ttcacccatg aaatgcgtgg caagcaggaa attctcctgc agcaccactt ccagcggcag
    13801 gttgacggtg aagccgtatt cggcatgctg catcacatgg gtggcattgg gcttgatttt
    13861 gaaaaagccc tgttccacca gtgtttttac cgaaacccct tcccatgtgg tgtcgaattt
    13921 gctccaacgg gtcacacagt ggatgtccat atcgattctg gaacgcggta atttgttgaa
    13981 ttcatcccac gaccagcgtt tttcctcctc cacttcgccc catacgcgga aatcccaggt
    14041 ggcggggtca aaggcgggga caggcccata atgcaggacg gggaatttct gggtgagaga
    14101 ttgccccggg ggaagccgac cctcggcacg gactcggtct tcttctttgc ggcggtcaaa
    14161 accttcaaat gcaaacatgg cttacctcac tttctaaatc ataaaagttg aacgattgcg
    14221 gcattgcatt gaccttttga atttgacaga tgttacctgt tgaaaatgat agttttctta
    14281 atgatgccta gtatagatgt aatgaacttg caggtcaatt ttttgacggg ctgtggttat
    14341 aataggccaa aatttcggcg tgaggagaag agatgaaggc acccactttt aaacaacggt
    14401 ttcagtattg gttcgataat tacatgtcac aggggactgg tgcattgatt ggaggattgg
    14461 ctgttgtatc tgtggtcatt atttcgatcg cggccctgat cattgccatt ggcggacgtt
    14521 tcctggcgcc tgaaggtgca gaaccacccg ggtttgttga agccttctgg ctcagcctca
    14581 tgcgtaccct ggatgcaggc acaatgggcg gcgatgcggg ctggggcttc cgcttgatga
    14641 tgctgctcgt gaccatcggc ggtattttca tcgtctccac cctgatcggt gttttgagca
    14701 acggtttgga agaccgcctt gaagaactcc gcaaggggcg ctctgttgtc ctcgaatcgg
    14761 atcatacact catccttggc tggacacccc aaattttcac agtcatctct gaattggtgc
    14821 ttgcgaacga gagccgcaag tccggggcgg tcattgttgt tttggccgat caggacaagg
    14881 ttgagatgga agatgcgatc gcagagcgga ttccagatac gaagaatacc cgtgtcatct
    14941 gtcgttcggg aaatcccatt gatcttacag accttgagat tgcgagcccg catacggcgc
    15001 gctcgatcat catccttgct gaaggcgccg acccggatac gcatgtcatt aaatcggtgc
    15061 tggcgatcac caacaatccg aatcgccgcg aagaacccta tcacattgtc acccagatcc
    15121 gcgacccgaa gaacatggat gtggtcaaga tgcttggggt aaaggacacc gttcagccca
    15181 tcctcaccaa cgacttgatc gcccgtgtgg tggcgcagac ctcacgccag agcggtttgt
    15241 ccattgtgta taccgagttg atgaattttg gcggcgatga aatttatttc aagcaggaac
    15301 cagaactttc gggcaagacc tatggcgaag cgctggaagc ctatgagacc tccacattga
    15361 tgggcgttcg caaagcggat ggtaccatcg ccatgaatcc atcaatggac tcccgcattg
    15421 aatcgggaga ccagatcttc gtcattgcag aagatgatga caagatcaaa cttacaggga
    15481 ttggccgcaa acaatttgaa gaacgtctga tccaggtttc cggagcagcc tcccgctcca
    15541 agccagagaa gactctcatc ctgggttgga atcgttccgg ggcaacgatc atccgtgagt
    15601 tggataatta tgtagccaag gggtcacagg ttacgatcgt ttcagacatt tatgagttgg
    15661 ataaacagat caaacttgat agcggtgctt tgaagaatca aaagatcacg gtgaaggaag
    15721 gcgatattcg cgaccgcgaa ctgcttgaaa gccttgaagc caccgaatac gatcatgtca
    15781 ttgtgctggc gtacaaccac cttgaacagc aggaggcaga tgccatcacc ctcattaccc
    15841 tgttgcacct gcgtgatatt gccgaacgtg attcgacccc gttctcgatc atcagtgaaa
    15901 tgctcgacct gcgcaaccgt gaacttgccg aagccgccaa ggtggatgat ttcattgtca
    15961 gcgagcattt gatcagcttg atgatgacgc agctttcaga aaactctgaa ttgcttggtg
    16021 tctttaccga tatgttcgac cccgagggtg cggagatcta cctcaagccg atcagcgatt
    16081 atgtggtgac gggtgaacct gtcaactttt acaccgttac tgaggccgca cgccgccgcg
    16141 gtgaaaccgc catcggctat cgtgtgatga gcgaaagcca tgatgccagc aaggcatacg
    16201 gtgttcatac caacccgaag aaatctgaac gggtcagtta ctcgcctgat gacaaggtca
    16261 ttgtcatcgc agagggataa tcattttgta gaggcacagc agcgctgtgc ctctactttt
    16321 taattttttt caaagatcga aatggattcg atgtgatagg tttgggggaa aagatcgaac
    16381 ggtgtcactt ccaccaattg atacccccca tgaataagcc gtgccgcatc ccgtgccagt
    16441 gtggaggggt cgcaggatac ataggcgatg acctgtggtt tgatggcgat cagggcatcc
    16501 aatgcatgtt tttcgatgcc cgcacgcggt gggtccacga tggcgcaggc ggcatccttg
    16561 agttggtcgg ccagggcggg cagaatttct tcagcggccc cttcgtagag ttcgatattt
    16621 tcaaattcat cgagattgaa ggcgaaatct tcacaggccg atgcagaggt ttcaatgccg
    16681 accacacgtt tgaatttctg tgcaaaaaag gcgctaaaca gacctgctcc acaataaaca
    16741 tctaacaggg tggtggtttc gttgacaggc agttttgaaa cgaggtgttg gaccatggcc
    16801 tccgccattt ttgtgttgac ctgaaagaaa gatggcgccg aaacacggaa gtctcggttt
    16861 gagactttga tggtcaaatg gtcttccccg gcgatgacga ctgggtggtc ttcaaataaa
    16921 tgcacaacgg atacatctgc ttcgatttca agctcgggtg tctctggtgt gtccgattcg
    16981 aacacgacca ttaactcacc gtcctgtccg ctacggagtg aaacgcgctc cacgccttcg
    17041 ttcgattcaa attgcaggtc aggccagaaa gcatggatgc ctgcttccgg taaatgacat
    17101 tcttcaatgg gcagggtgga gttacccttt gcatgaatga atccgagcct gccctctgcc
    17161 gtgaggtgga attgcacatg gttgcggtag ttccattcga gcggtgaggg aatcattggc
    17221 ctgactggcg gattttcgat tttgccaata cgctgtaact ggtcgcgcag gatttcagtt
    17281 tttgcggcca gttgtttttc gtatggaagg tgctggtaat gacacccgcc gcacacccca
    17341 aagtgtttgc attttggggc aattctctcc ggggaaggct gcaggatttc gagcagtgcc
    17401 ccgcgtgcaa aacctttttt ctcctccacc aggtccacac gcactttttc gccgggcagg
    17461 ccgaagggta cgaacaaggc acggccatcg ggaagccgcc ccatggcttc gccgccgtag
    17521 gtcatttttt cgagcaggat atcgtataca ggtttggtca tggtttttga aggaaaaata
    17581 aaaagaggaa aagcacggaa tccgtctttc ctctttttgg gttttgccgc tccgtgatgc
    17641 gaggattatt tgataacgaa ggaaagcgca accaacagaa taaagaatgt gcctgaaagc
    17701 agaccgatac gggtaaggtc cttcttcacc tgggtgtaat cagggttgaa ttccgctgcc
    17761 gtctgttggg gtgctgcggg tgatacacga gccggtcttt tattttttgg tgccatttta
    17821 tatttctcct aattttgtaa tcgcgcaaat acaacaatgc gctgtttgtt gacaaggtac
    17881 ggatagcatg agatcaatgt tacggtcggt gtgccggtgg atgccatcac ctccaccgcg
    17941 gttggttcaa ccagaatggt gcggtcgacg atgtaggtat attgccgctg ctgtgtgtac
    18001 acgatgacct ggtcaccggg cgcaagacgg tcaagatatc ggaagatctc tccgtaaaca
    18061 tcgttgtgag ccgaaagaac aacgttccca tcctgacctg gatttgagga accgatattc
    18121 tggccaacgc ctttttttag ctgttcccat ccatcacctt gaacgatcgg ggcgtcaata
    18181 ttaagcgccg ggatctgaag gcgtaccgcc tgatctggtg cggcggtggg gacaggcagg
    18241 ttcaccagtg actgcaccat ggggcgcagg tggtcgggaa tttccgcttc gtttggccgc
    18301 gtgttgccct gcgcatcggg tggggtatga cctgatggta atacaacggc catgaccagc
    18361 ggggtcgggg taacggtttc agggttgagc gcagacgcca cttccgtgtt gagagtacgc
    18421 aacatgccga agctgttcaa taatacggcg atcaaaccaa ggacggcgag tacttccaca
    18481 ccgagcaaga cgcgatccat gaatttccga cgcgggttga ctggaacctg ctccggggag
    18541 gattctgctg cttcggctgt atcaacaatc ggtgcagcac gatcagactc cgcctcggat
    18601 gggccgggcg aaattgagag gactcttccc gtgcggcgga agtattccag tctgtctttg
    18661 cgcgcaccac gtcgtttttc aaccagcagt cggcgcaatt cctcgacgga tagatcttcg
    18721 ggtgctttct tgcgggccat gtcggtggga ttatacaggg gattgtcaat ttttgggaca
    18781 ccttgtataa tggcaatcct tcgggcgggt agctcagttg gttagagcac tgctttgaca
    18841 tagcagaggt cgtaggttcg agccctatct cgcccacaaa aaagtcccat tttgggactt
    18901 tttgcttttg cttgacagcc ttttgaatca gcatataata ctggctatca ataaatcata
    18961 cgcgttgacc aagacgagta cgtgaccatt gttctgacag agagaagaag ccagagactg
    19021 agagcctctt ccagaagcaa tcaccgaata ccacttgcga gcgtgacctt gaaatcgaaa
    19081 gagagtaagg gaaacgggtt gctccgttag aggcttaaga gtggcgtgtt ggggaaacct
    19141 caacatgcaa ttcgggtgga accacgtgag attaaactcc cgtcccgttg tggatgggag
    19201 ttttttattt taggaggtcg ccatgaccac atatactcgt tggcggcccg gaggtttgtg
    19261 actgctcacc tgcgctacgg tgcaagtgtt tctccggggc gcgaaatgtc catgaagcag
    19321 gatcaagcca aagttaagaa aggaagcgaa aatgtctcaa attacagaac gttatgaaga
    19381 ttcacaactg tatcggatcc gtcattctgc ggcgcatgtg atggcgcagg cggtgttgga
    19441 aatgttcccc gatggcaagg tgacgattgg cccgccggtg gagaatgggt tttattatga
    19501 tttcgatctg ccgcgcaacc tgacgcccga agatctggaa gccatcgaaa aacggatgcg
    19561 ccagatcgtg cagggaaaac acgagttcag gaaaacggtc atctctgcgg aggaggcgaa
    19621 gaagatcttt gccgaccagc cgtataaact tgagttgatc gagggtcttg aaaaaggcgg
    19681 tttggacgaa tacggcaacc cgctggccga gaagccggat atttcgatct accagcacga
    19741 tacgtttacg gacctgtgcc gcggtccgca tgtggcgaac acgaaagaga tcaagcagga
    19801 tgcctttaag ttgatgtcga tcgcgggtgc atactggcgc ggtgatgaaa acaataagat
    19861 gctccagcgc gtgtacggca cggcctggga gagcaaggat cagttgaagg aataccttca
    19921 acagcttgag gaagcaaaga agcgcgatca tcgcaagttg ggcaaggaac tggaaatctt
    19981 catctttgat gatgaggtgg gtcccggcct gcctttgtgg ctgcccaatg gcggtgtgtt
    20041 gatcgaggaa ttggaaaacc ttgccaagga aatggaactc aaggctggct atgaccgcgt
    20101 gcgcacaccg aatgtgacga aggaagatct cttcctgcgt tcggggcatc tgccctatta
    20161 tgccgagagc atgtatccgc cgatggaatt ggaaggcgtg aagtattaca tcaaaccgat
    20221 gaactgcccg atgcaccaca agattttcgg ttcaaaggga cgttcatacc gtgagttgcc
    20281 tgtgcgtctg gcggaatatg gcacgtgtta tcgctacgag aaatcaggcg agttgttcgg
    20341 cctgatgcgt gtgcggtcga tgcagatgaa cgacgcccac atttactgct ctgaggaaca
    20401 atttgagcag gagttcatgg gtgttgtgga actgtataaa aagtatttcg atctgttcgg
    20461 catcgaaaag tatgtgatgc gactctccct gcacagcaag gcggggctgg gtaagaaata
    20521 tgtgaacaac gaagcgttgt ggatcaagac ggaagacatg gtgcgccgcg ccatgaataa
    20581 cggcggcgtt ccatacgtgg aacgtgaaga cgaagccgcc ttctatggcc ctaagatcga
    20641 tgtgcagatt tggtcggcga tcgggcgtga attttcgctc gcgacgaacc aggtggattt
    20701 tgccgtgccc gaacgttttg acctgaaatt cacgaataag gaaggtcatg acgaggtgcc
    20761 gctttgcatt catcgcgctc cgctttccac gcatgagcgc atggtgggtt tcctgttgga
    20821 gcattacgcg ggcaacttcc ctgtgtggct ttcgccggaa caggtgcgcg tgatctcgat
    20881 cacggacggt cagaatgagt acgtggataa gatcgcaaga gagttgcgcg agaacggaat
    20941 ccgcgccagt gccgatgttt catcgcagcg catgaatgcc aagatccgtc aggcgcagtt
    21001 gatgaaggtg ccgtacatga tcgtggtcgg cgataacgaa atgcaggcga atcaggtttc
    21061 gctgcgcgca cgtgatggca gccagcagaa taatatttca ttgggcgagt ttattgcgcg
    21121 ggcgaaggat cgaatttcaa gaagggcgaa agagttgtaa aagaaagaag aaagagacag
    21181 gcggaggatt ttcctccgcc tgttttttta ggagcatttg tgggtctatg gcatccagcc
    21241 gcggaccatt tccacgctga gggggaaggg gtggatgcgg caatcgttta cgttgaccag
    21301 cgccatttcg gtggggcggt cgggcaggtc ggtggagcgg ataatgacga tcagccaatt
    21361 cccatccggt gaccagaacg gcgcgccaac gatccaggtt cccatttgtg cgaacagtct
    21421 gcgttccgag ccatccaggt tggagatgta aattgcatcc tgcaggtttt cacgcgcatg
    21481 atatgcaatg tgggtgccat cgggagaaat tgcaacatct tcgtatgtgc ggtgttgagg
    21541 aatggtgaaa agttcggcca ctgctccact gacgagatca atttttcgca gggaaatacc
    21601 gccctccacg ctcacaccat agaacaggcc ggtgccgtcg tttgtccaat ctatcaattg
    21661 ctcatcacca ttcaaatcgg ctacccgtgt ccgctcgctg ccatcgggat tgatgatata
    21721 tagattgagg tcggttccgc tgatgaaggc gatctttgcc ccattcggcg accagaccgg
    21781 gaaattatcg tcagaggtgg tgttgggaat gtggaattgc gcaccgctgg cgatttcggc
    21841 aatgttcagg ccttcacccc atgcaaaagc cacgaatttc ccatccggtg aaagcgaggg
    21901 gaaaacgccc tgcgagatga tctgcttgtc actgcggtca aggcgcgaca tgaaactacc
    21961 atagttatcg tagttgagcc aatcgtcact gagggctcca tagaccagca gcctgccgtt
    22021 cagttcgggg ggaactggtt ggtagttatc caatgccgct tgccacgaat cccgcgtcag
    22081 gcaggtttga tccgctgttg tgggtgtgga aactgattcg gtggaggaaa tctccggttg
    22141 ccattgaatg ttccagtttc cggagatcgc catcacgggg ttggtaatga gcacttccat
    22201 ttttcctgaa gggatggcac cgttgaaccg caccacggct ttgaaatgat tcgggtcatc
    22261 catgcctgtg ccgccgggca atgaaccttc acccattgtc aggctgacgc catggaaaaa
    22321 ggcagaactc tcgaagttga agacatagcc ttctacagtg cgggttgctt tgacgactcg
    22381 caccgggaat ccgaccaact caacatcttg attcagatcc caaacctgcc cttcctgtgg
    22441 atttgccccc gcatcaaact cgaagctggc gcttgatcct gaatattggg cagccactgc
    22501 gctgacggtc atggtcagcg ggtaggcgtg gtctttgcca atgatttcaa aagcccacgg
    22561 gaaggtgccg attggtggaa attcagcagg ctggtcaagc tggaaattgg caaaatcata
    22621 aatgacctcc tgtccattgg catcggtgaa aatggggaag gttgagaaat ccatggcttg
    22681 agcgccctgc ggcaggccag tcgaatcaaa ggtgccaaca agaatatagc ctttttctgt
    22741 ttccgcaatt ttttccagtt ttagcgggga tccttcgaca ggttcctgcg aaggtgtcac
    22801 ctcgatcact ggggcaacgc tcatctccgg cggtgcaggg atgaagtgca ggggcagttc
    22861 ccaattttca ggcaatgttc ccgagagcga cctttcgatg catggaatga caagcacggc
    22921 atcattgatc tctgcaggga gtgcggcaaa gatgtggcga acttcatacc ccgtgcccca
    22981 gccattcatt tcatagccca ggtcccttgg catgggatct tccacagttt tcaggaatgt
    23041 gtgctcttca ggtttcgagc cgcaaccggg gaaggccatg tcctgcgaaa gcttttcctg
    23101 tggaatgttt tccaccttgt aaagcaccac ggtcttgctt gagctcaaca ccacgccgct
    23161 gactgtgatc gtgattccat cccgagtttg tgtaacaggc tcagccagta tgcggatggg
    23221 tgtgctttga tccacgatcc ccacaccggg aatgtaacca agagaacgcc cgatggcata
    23281 ggctactccg cttaaggcga ttaagataat caatgcaatc agcatcgcca caagcctgtg
    23341 ggtgcgaaat gtaaacatcc gggaattcct ttcgagacgg gctgcaaggt gcggcgtgag
    23401 gtttgtgctg tcagggatgt ggtcttttgc gatatcatcc agcaagttga aaggtctgtt
    23461 gtttttcatt ttcagatcct ttccttcttg agcaggtcac ggagggtgtt tcgggcggag
    23521 ttcaaaagcc atttgacagt gccgggggcg gcgtcaagtg tgaatgccat ttccttttcg
    23581 ctcatatcga ggtaatacct ttgcacaatg gctgcacgtt gccgtggcgg gagttgcccc
    23641 agtgcattca atatctcgct tttgagttgt gaatattccg cctgagattc aaccgaagcc
    23701 tcttgcagga tcagtcgctc gatggtttct gcattggctt caagcgagat aaaacgtttt
    23761 tcgccgcggc tggcattcaa tgcagcgtta accacactcc gcattagata gggttcgaaa
    23821 ggacggtttt cgtcaaaact gcctggacgc tgaaagatgc gcaggaagac ctcctgaacg
    23881 acatcctccg cttttgcttt atcgcatgtg ataagaaacg cggcgcgcgc cgcctttact
    23941 tgatacaacg acattaaata ctccaacccg ccaaaatcac ccatctttat gcggcggatg
    24001 gctgtaactt catccataca attctccgtg ttgttgagcg ctcatacttt aattacacac
    24061 gacagctcaa aaaggttggc aggaaattcc tcaaatagga aatgcaattt ccatagagtt
    24121 tgaattaaaa cacacaggca ctcggttatc cgaatgcctg tgatcgtttc atttgttgaa
    24181 ttgttatggt acccagttgt agacatcccc gctccatgaa aaggggatga tctggcagtt
    24241 tgtcacgcta accaacgcgt gcggaattgg tagcccg
//
LOCUS       DAOQLC010000067        23418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_735_length_23418_cov_23.787142, whole genome shotgun sequence.
ACCESSION   DAOQLC010000067 DAOQLC010000000
VERSION     DAOQLC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 23418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_23418_cov_23.787142"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="atpG"
                     /locus_tag="PL000_12665"
     CDS             complement(<1..857)
                     /gene="atpG"
                     /locus_tag="PL000_12665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNC89799.1"
                     /translation="MASAREMRLRIKSVKNISQVTRALETVSASKVRKAINAVMATRS
                     YATKAWQVLRHVAEQPGRDSLHPLLVKRSNVKNALVVVITGDRGLAGAYNSNVIRYTA
                     QKFDQYSVPVKYIAVGRKGRDLLFRRRKDVIADFSNLPAAPTFMDVSAIGRLAVEEYN
                     KGNVDEVFLIYTDFINMGRQVATVKKLLPLELEGEGLVEDFEHKASSGPSAAYEYEPD
                     QREILDEIIPRFTALQVYQAILESQASEHAARMIAMRNATDNAKELVSALQLVYNKVR
                     QQTITNDIL"
     gene            complement(886..2544)
                     /gene="atpA"
                     /locus_tag="PL000_12670"
     CDS             complement(886..2544)
                     /gene="atpA"
                     /locus_tag="PL000_12670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC89800.1"
                     /translation="MSDLIKQITGDLQKQIEGFKPELNVSDIGVVVEAGDGIARVQGL
                     ANVRAQELVQFSNGVMGTAFNLEQDSVGVIVMGEYDEITEGMTVRGTGRIASVPVGDA
                     MIGRVVNALGEPIDGKGPIATTGFRPIERIAPGVVERQDVDTPVQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDAIINQKGKGVVCIYVAVGQKKAAVARTLGILEKYGAM
                     EHTIIVMASADESAALQYIAPYAGTAIGEEFMETGRDALIIYDDLSKHAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERSARLANQFVVVKKDYSNDLADAKDGVDGKVYTGP
                     LSKHEAEEAAKAMGDGFKAVKVKGTGGSLTSLPIIETLLGDVSAYIPTNVISITDGQI
                     YLESNLFNAGIRPAVNVGISVSRVGGDAQTKAMKQVAGRLRLDMAAYRELAAFALMAS
                     DLDKSTQQQLGRGQRMQELLKQPQYQPMEFEYQVIVIFAGTNGYADSVPLDQMAQWQT
                     SLIRYMETSFPEIGKDIVAKKTISDENRAALAKALDGFRAGWQA"
     gene            2817..3956
                     /locus_tag="PL000_12675"
     CDS             2817..3956
                     /locus_tag="PL000_12675"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89801.1"
                     /translation="MSEINREEGKPPSRRMKRYWKVAVLANIKDDNQPKPEGVPPDAF
                     ADFDHIETIDSLRAAIETDGHTTYFIQADRDLPFALREIQPDICFNIAEGLGGDAREA
                     QVPALLEMLGIPYTGSRVLTNGISLDKTLTKRIWRDRRLPVAPFQEFNAGDEPLRPEL
                     KFPLFVKPAREGTGMGVDLKAIVNNEKELRERAQYIVNVYQQPALVETFLPGREFTVG
                     ILGRADSKLYSRHPEWYEKDGFHRFPVLELDSTRSVTPWVYGLEAKSKDVGADGSPGY
                     FCPADIEPELEKKLKNFALRAHQLLNTLDVSRTDIRLDEDGNPRVMEINTLPGLTPDY
                     SDLCLQAKAEGIRYEDLVLDILYLGASRWGMLEPREYVPDTPKKR"
     gene            complement(3999..5591)
                     /locus_tag="PL000_12680"
     CDS             complement(3999..5591)
                     /locus_tag="PL000_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNC89802.1"
                     /translation="MQTNNRLGCLTGTGIFAALVTIAAIAGLAFFSGSQMFTAGALNA
                     EPGDEVLGGVNSHAQITECSACHAEPWSATRMADHCAKCHTDIAAQMYDVAQLHGAIM
                     QKNNTLACRDCHPEHRGASASLTEMGENTFPHEALGYSLEGHQQKATGEAFICKDCHG
                     EDVTTFASNSCDNCHRQMDIVFTQTHVLSFGTDCLACHDGIDRYGDNFTHDQFPFQLK
                     GEHAEANCTECHLDARSIADLQSLPQDCFSCHREDDPHGGAYGNECGVCHAPEGWLPA
                     KFDHNLSAFKLEGEHNEVACEKCHVNNVYKGTPSDCYSCHQQDDEHNGRFGTQCETCH
                     TPKNWESANFDHNLTAFKLDGEHREVRCERCHVNNVFKGTPTDCYSCHQQNDEHNGKF
                     GTDCASCHNTNDWDDATFDHSRTNFPLTGGHSGVDCEQCHKGDSFTGLSNECVSCHAD
                     PAFHAGVFGTNCATCHTIFAWTPAGFNIQHPEPIAEEGGNGVFHGGASCRQCHPSTVQ
                     EATCTACHDGNDFEGGEGGDDD"
     gene            complement(5601..6155)
                     /locus_tag="PL000_12685"
     CDS             complement(5601..6155)
                     /locus_tag="PL000_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89803.1"
                     /translation="MLRGNKELWLAFVFCLLITGLYGAVVIWTREIPAAGELFGHSIG
                     IVGFVLMLMTEVLYSLRKRSRSAALGRMSTWLQFHIFTGLVGPFMVLLHTSWKFNGLA
                     GATTLLTVIIVFSGFIGRYIYTRIPRTLDGLEIEGTLSQEALKQARRLMSLWHTVHIP
                     IGMALFISAFIHMGAALYYATFLR"
     gene            complement(6206..7504)
                     /locus_tag="PL000_12690"
     CDS             complement(6206..7504)
                     /locus_tag="PL000_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC89804.1"
                     /translation="MRYIIIGSGVAGVAAIEAIRKNGMSGEIVMVGDDPHGFYSRPGL
                     AYFLTGELHDKALFPRTREDYVRLHFKFHKSRVTKLLPSEKAIELDGKSHLSYDKLLI
                     SVGAQAIPLTVPGANLEGVVKLDHLEDARKILKFARRGKTAVVVGGGITALELAEGLL
                     SRGVKVHYLLRGDRYWSNVLDDFESRIIERRLQEEGVTLHFHTELAEITGRSRVNGIR
                     LKDGRAMRCDMVAYAIGIKPRIELARNAGISVDRGILTNEYLQTNHEDIFAAGDVAQV
                     FDPVTGRSVIDSLWHLARDQGHTAGLNMTGERKTYIRNTPFNVTRLAGLTTTIIGSVG
                     HGHDDDIVGIARGDSETWRQLPDAIVAQSGFDVNRVRLMVGETTLIGAVVMGDQTISA
                     PLQRIVRDRMDITSMRDKLLARDAKIADVIVDFWSKYKTN"
     gene            complement(7501..7767)
                     /locus_tag="PL000_12695"
     CDS             complement(7501..7767)
                     /locus_tag="PL000_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC89805.1"
                     /translation="MSFINIFKTDSTKRVALSAGHVVPDSSRCVQCGICTYNCPIGID
                     VRAHAWEGQAIKHSECLTCGECVARCPRGVLRFERTDLFAEGGK"
     gene            complement(7942..8154)
                     /locus_tag="PL000_12700"
     CDS             complement(7942..8154)
                     /locus_tag="PL000_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC89806.1"
                     /translation="MSERFVGTVKWFNSTKGYGFIGRDGGEDVFVHFTAIQSDGYRKL
                     EAEQKVEFSIEEGPKGLQAANVVPLS"
     gene            complement(8261..8929)
                     /locus_tag="PL000_12705"
     CDS             complement(8261..8929)
                     /locus_tag="PL000_12705"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC89807.1"
                     /translation="MTLPKKLTLEFHCHTHASKDSLTHPKDLVQAARRKGLDRLVITD
                     HNSIAGARAAQALDPELVIVGEEIMTTKGEILAAFVQEEIPPFLTPQETLMLLKAQGA
                     FISVSHPFDELRAGGWQEKDLLDVIPFVDAIEVYNSRCMLPRFNRKALEFARLHGLAG
                     TVGSDAHAVFEVGRSVLLLDQFAGPAEMRKVIRSAEFRVKWSPWWFHFVSRYASIRRR
                     FLKS"
     gene            complement(8926..>9743)
                     /locus_tag="PL000_12710"
     CDS             complement(8926..>9743)
                     /locus_tag="PL000_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HNC89808.1"
                     /translation="WMKGCMDWLLPNKEVEAINSWREAEKRFKAIRDKENKKSRLDWY
                     DARCKEMHDALHQAAEKYQIPPLPAETPENKSLFGEMATNNSREDAEQQVRSDRVGLF
                     SIHESINAGDFGPSGMLNTPIEGMVEVEQVFIEDKPYRIVSVDGHSFFRSSTHGTAVI
                     KVSGDSMNLANIGSGDYVLLRFLPKNPQVIAEPDESGDMPPLTSYRNGDIVAAEIIND
                     EGDGVTLKRIVVRGGRIVLQPQSSNPVHVEREFNISDPHLYICGVVQAIFKPL"
     assembly_gap    9744..9843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9844..10229)
                     /locus_tag="PL000_12715"
     CDS             complement(<9844..10229)
                     /locus_tag="PL000_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89809.1"
                     /translation="MMTTETKSIRWLKYVSGWLNKFNKSSLSDPSNLLLERLRRECDR
                     KEMKKAWSIIEDLDRLRQASDGLEPAEICLQCGLSVAEMGNFREAQRLFSEASTKYLN
                     SFHHQAVALWMKGCMDWLLPNKEVEA"
     gene            complement(10226..11683)
                     /locus_tag="PL000_12720"
     CDS             complement(10226..11683)
                     /locus_tag="PL000_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC89810.1"
                     /translation="MMIFRSAKTRVSRILQNLLVGDLIWARLRGLFLLFATAAVSVFI
                     AAGFSADKLTLLRIWNDIAFPMIWLAASILLSAYILVERYEEERAKPLKQVWYVIVFA
                     IFIGPLSWFEFVYVRPLLTGEPISRFLILPLAALISAFLSGARYVQDVFNIKHYVLAV
                     LYLLSSFCGIKYPKIKISENYVVGDGKNRIDQIGGPGYVFIGPGNAILTERLHGPAGV
                     YSAGRFFLSRFETIVSTLNLDDQHGLIPAISAVTKDGIMVTVKDVQFRYRVWSGHREI
                     NTAAGRSLTNPYPFTVQAIRNLTYNRTVSKSGPNTWHDMVKGVISGAVSDYITKHQLD
                     QITAPRYTDGDARKEISAQLKTSSTRDKLKDAGAQLLWCDIGHFEVDNKAVSEQRIDT
                     WKAGWMGNADVKRAYGEAQRNAYQEIGHAEAQAEILMSIVHAFDDIDLAKEDKDRSIR
                     NIILMRTAQVLEALSVSEGSEQNTDKNAKKDGKKP"
     gene            complement(11680..13470)
                     /locus_tag="PL000_12725"
     CDS             complement(11680..13470)
                     /locus_tag="PL000_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89811.1"
                     /translation="MPADTRISRQARLSILGTAVLVISLSGLSFFIEIKLVWQLVISW
                     GYVYILRAADAVYVSPEASLASSMLFANISVFVLIFFVVLFWVSPFILPVRGLVQTQK
                     AFTGLIRSLAGKKWHGPAIFVKEGKPNMEEHELDSHKAGVALVDLYSAIVLEQQTGSI
                     DSLRQEVHFEAEEEDVQGLQRPGMWKQITAKLGVAGKNVNVTFVKAFGPGVVFTESGE
                     KIVGFVDLRKQSRSVQGVKACTRDGIEITTNVNVTFTLGQAEDILKVTRTDLGWRAVQ
                     ITAPSAEDLVDRPEPRPGDQVVKFQPVDTINEQDMNELEHAFTEKEFEWSDTDIRIVK
                     VGAASQFVFEKERVFSAVYSRARSATGESQLIDWTELPGYVAADIFREQLAHENYDDL
                     YKPEEPNEFPMGDFKRKFGRMVTNQGMLGYQVVMLADETPIKDAQFVNEADLKFSPPL
                     RFKGPAILRDRGIKVLSAGFGDLVPVNEDVRAQLFENWRAHWQQEAQKTLANHELRAM
                     RIHNHERSRTQQDMIYSLSRIFHENQYTEEALALRLYQALEAAAINPATQRLLPADTV
                     QMLSNLRHWLLPENKKSDESNPVIGEEGTP"
     gene            complement(13476..13931)
                     /locus_tag="PL000_12730"
     CDS             complement(13476..13931)
                     /locus_tag="PL000_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89812.1"
                     /translation="MENIGGLAAEIVVAFVIALATGAFTFMGFWRQAKADLEQEYLKR
                     FNDKKWGVYTEFAKLLQAPSLNTFETDHRQEAETAIASQIVLIGSDEVVRAFRDWRES
                     VAVHGKAQRVTNEKLFALVANMRRDLGIKYSNLEVDDLLGVLNPSMGDG"
     gene            14170..15930
                     /locus_tag="PL000_12735"
     CDS             14170..15930
                     /locus_tag="PL000_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89813.1"
                     /translation="MKTRNFLIRIGLALLVAALLGAGAGLLAREKVDAAEGTYKILAW
                     NDLGMHCYNRDFADLAVLPPYNTLWVQVIKAGDPPTLVTSGVTVEYFYEDNTYSVGKT
                     NFWSYSKDLFGVNLPPNVGLKGKGLSGTMDFSGDHFVAEGIPVTEFSDSAPTTSQPYQ
                     LATVIVKDSATGAELARTRVVTPVSSEMRCDKCHSDGGEASPDNPTGKVETNILQLHD
                     EEEGTNLMANRPVLCADCHASNALGAPGQSGIPNLSNAMHNKHEDESPNTLNGCYNCH
                     PGPETKCLRDTMSQEGMTCIDCHGGMSDVKNNPDPWLNEPRCDTCHDSGQYDQNHPLY
                     RFSTEHGGLYCEACHDSTHAVAPSREENDAIKFNILQGKNGPVDQCTVCHTTTPSTGS
                     GPHGLTSSGALFEDVPSTYWARDFIERLYNAGITSGCSTSPLMYCPDATVTRAQMAIF
                     ILRGMHGSAYIPPTASGTVFSDVPAGSFAADWIEQLAAEGVTAGCGGSNYCPDATITR
                     AQMAIFLLRGEHGNAYAPPAATGTVFGDVPLGSFADAWIEQLAAEGVTSGCGGGNYCP
                     DNSVTRAEMAVFLVRAFSLP"
     gene            16110..17570
                     /locus_tag="PL000_12740"
     CDS             16110..17570
                     /locus_tag="PL000_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC89814.1"
                     /translation="MNTKRLFSRIITILIVVIFFLSGTAPTLAAPAPIPLYADDPTPP
                     AQTVKLIFIHHSTGENWLADGYGNLGIALEQNNYFVSGTNYGWGPNAIGDRTDIPNWT
                     EWFRSGDTETYMNALFNESGQNSSFTRNLTDPGGENEIILFKSCFPNSALEGSPTDPP
                     SPDGWLTVGHAKYVYNEILQYFASRPDKLFIVITAPPLQDGTYAENARAFNEWLMNDW
                     LTENGYTLPNVAIFDFYNVLTGPDNHHRYINGTIEHVFTPGMNTSYYPSSADDDHPSA
                     AGSQKATDEFVSLINIFYNRWRNSTSSTFTDVPSTYWAAAYIERLYNAGITSGCSTSP
                     MMYCPLAPVTRAQMSIFILRGMHGSAYIPPAATGTVFSDVPADAFAAAWIEQFASEGI
                     TSGCGSGNYCPDANVTRAQMAIFLLKGEHGSSYVPPTATGIFTDVPIGSFAADWIEQL
                     ANEGITSGCGGGNYCPDADVTRDQMAVFLVRAFSLP"
     gene            17787..19091
                     /locus_tag="PL000_12745"
     CDS             17787..19091
                     /locus_tag="PL000_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC89815.1"
                     /translation="MSISNATPVLALDEKNLIPVSDHLKQMADIPPSRMFLINKSLKV
                     YVEKNAGAKIFDASQGDGGASLPGTPKAILERALQLQLEHGTAYDMPFGTEAYRKAVI
                     EQYWKLDSSSGWGPANVLGTAGGRDALVKVYGAMLALGHGRQGDLIMVSRVPWISYNW
                     GPYGVGANVLWAPGDPAQGWAYSEDAIRESVKFAESKGRKIAGIVITNPDNPTGLTIA
                     VEKQVSLAKAALEAGVAFVLFDWMYHYVTDESPMDLNSFLKNFTPEERKRLMFLDGIT
                     KSLGGSNIRNCHLIADEAVIKFIMARASHGVIPSFYSLAVAMAAYEMGFTEAAKTIIE
                     PTNASRAVLKKLLAESGMQHIIGKGYYAFMNVGEFIKAKGWADSEPLGQYLAENHGVA
                     IVPGAFFSPFGGDWIRFSYATPTERTEGAFKRLVEGLESLKK"
     gene            19186..21981
                     /locus_tag="PL000_12750"
     CDS             19186..21981
                     /locus_tag="PL000_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC89816.1"
                     /translation="MPQQFADKYLLAVEQAIKEKPQGGLDGFEQEWNLLDEELRPLLT
                     VGAGPSQHSFVDYLRAECVPHWHQQFSQLEVFHWMVEWATRPYYTPRGAMYEARLMEA
                     TLMNALHRAGVNFGERLHYWHGNLLFLTDIGHQSIPGNWSLAKRRYLEKCVDLYGDTL
                     ATTGIHTNMSLPDPLFAWDFMHLSPSERGDQHLDEFKSEFYITATRLLRAFASLFIAT
                     SASTPMQAQVKDGRAAVILTEFDSIRNLTFPNPREIDLPDLYRSYNDYLQISYDLVRR
                     GVRFGNNNWTPVRARSFSEPVERIISTTSDQLSSLYARGLFAAGQSTPPEEMARQVER
                     QNLMARINLPMGRVEVRVDEGGHSLDLDIANMTFRHLLLLKIYSDPQFARGFRYDRED
                     ITRARANENMAAKHGLRAEIENPLTGKPITVREFLKWTLNELKPLAQGLNMWDDLHPL
                     VEMSEGGRNTSEKIRARLKMEIGDDNEVPLPLLKEFFYEHEAQVKADVETICANHASL
                     GADSAKIAEFIQRSRDTVRSSEIPNAPVQFRPRAQAVIEISYPNKTAEILDLAQQLIR
                     IPSVTACPVERFEEVHRAGSLIDDYLRNAGLDVKYFDGKYPAIYATFPGASKPNPILL
                     TGHFDVVEPEPDDSQFNPRIEGDYLFGRGAADMKTVVATYLVWMKDMCKSASRPNIAL
                     MLVGNEENGEAEAWGTPHVLKELNLTPSLFIAGERTGEKGNELFGEICVENRGVMRFD
                     VIARGAKGHSGVAGTGDLSEKLIAARSALNEIFASHLTLKAADGWQSQAKFPFITVGT
                     PGVYNVTAAEGILGVEIRPIPQDDVTGLRLKVESYCAENGLEASFNVMENGVACDPNN
                     PALKALIEAVRQASGGEEPRIGKKLPGTSARFAPGGQAVVWGQSGVGPHAKNEAHYIP
                     SIEPYYNSLNELAKLWK"
     gene            complement(22050..22511)
                     /locus_tag="PL000_12755"
     CDS             complement(22050..22511)
                     /locus_tag="PL000_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC89817.1"
                     /translation="MPDDVFQFDNADDSPGFSLWQVSSMWQRQINAGLKPFDLTHAQF
                     VLLASLMWLANEDKPLTQVDLANHAKMDVMMTSNVLRTLEEKGLILRKPHPTDTRAKA
                     LSITAQGRKLAGQAVPAVEAIDRKFFSQLGADARRFNEQLLKLIVANYADG"
     gene            complement(22504..22941)
                     /locus_tag="PL000_12760"
     CDS             complement(22504..22941)
                     /locus_tag="PL000_12760"
                     /inference="COORDINATES: protein motif:HMM:NF002616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNC89818.1"
                     /translation="MSEARYWLIVVSKNHVMNGVRWGIAQANHGKAAPLKRMRVGDGI
                     VYYSPKVEFEGNEKLQTFTAIGEVTGETVYQFDMGGGFVPYRRDVKYKECVEVPIQPL
                     IPALTFIKDKTSWGYMFRFGFFEIPKIDFDLIASQMLTEKINA"
     gene            complement(22938..23357)
                     /locus_tag="PL000_12765"
     CDS             complement(22938..23357)
                     /locus_tag="PL000_12765"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC89819.1"
                     /translation="MWTTEHTLVTDASKEAVWKVWADVENWSKWDKGVEWCRIEGDFK
                     AGTKYTLKPVGGPEAKAVIIDCQPLSRFVDVTSLPLAKMEFAHELKEEKDGLHVTHRV
                     TISGPLSFLFAQVIGKDTARDLPETIGRLVRLAKESK"
BASE COUNT         5968 a         6517 c         5382 g         5451 t
ORIGIN      
        1 tccaaaatat cgtttgtgat cgtctgttga cgaactttgt tataaaccaa ctgcaacgcg
       61 ctgacgagtt cctttgcgtt gtcggtagca ttgcgcattg cgatcatgcg ggcagcatgc
      121 tcacttgcct gtgattcaag gatcgcctga tacacctgca aagcagtaaa acgcggaatg
      181 atctcgtcca aaatttcacg ctggtcgggt tcatattcat acgccgcaga aggaccgctg
      241 gatgccttgt gttcgaaatc ttccaccaaa ccttcacctt ccaattcaag cggaagaagt
      301 ttcttcactg tagccacctg acgacccata ttgatgaagt cggtgtaaat gagaaacacc
      361 tcatcaacat tgcctttgtt gtactcttcc acagccaggc ggccaatggc agatacatcc
      421 ataaaagtgg gggctgcagg caaattgctg aagtctgcaa tgacatcttt acggcggcgg
      481 aagagtaagt ctctaccctt gcgtccaacc gcaatatatt taacgggaac agaatattgg
      541 tcgaattttt gagccgtata acgaatcaca ttggaattgt aagcacctgc aagtccgcga
      601 tcaccagtga tgaccacaac caacgcattc ttgacgttgg agcgcttgac gagcaacgga
      661 tgcaaagaat cgcgtcctgg ttgttcggcg acatgtcgca acacctgcca tgctttcgtc
      721 gcgtaggaac gcgtcgccat gaccgcattg attgccttgc ggaccttgct ggcgctcact
      781 gtttccagcg cacgtgtaac ctgtgaaatg ttctttacgc tcttaattcg gagccgcatt
      841 tcacgagcgg aagccataga ttaatccttt atccttttga aaaaactagg cctgccagcc
      901 cgcacggaaa ccatcaaggg ctttcgccaa tgcagcacgg ttttcgtctg aaatggtctt
      961 cttcgccaca atatccttgc caatttcagg gaaggaggtt tccatgtagc ggatcaggga
     1021 tgtctgccac tgagccatct ggtcaagcgg aacactatca gcataaccgt tggtgccagc
     1081 gaaaatgaca atcacttgat attcgaattc catcggctgg tattggggtt gcttgagcaa
     1141 ctcctgcata cgctgaccgc ggcccaactg ctgctgggtg gatttatcaa ggtcagaagc
     1201 cataagagca aaggcagcca attcgcggta ggcagccatg tccaggcgca gacggcctgc
     1261 aacttgcttc atcgctttgg tctgggcatc accacccacg cgggaaacgg agatacccac
     1321 gttcaccgca ggacgaatac cagcgttgaa aaggttgctt tcaaggtaga tctggccatc
     1381 agtaatcgaa ataacgtttg tcggaatgta agcagaaacg tcaccaagca gagtttcaat
     1441 aatgggcagg gatgtgagtg aaccaccagt tcccttcacc ttgacggctt tgaaaccatc
     1501 tcccatcgcc ttggcagctt cttcggcctc gtgctttgaa agtggccccg tgtatacttt
     1561 gccatccaca ccatctttgg catcagcgag atcgttgctg taatccttct tcacaaccac
     1621 aaactgattg gcaagacgag cggaacgttc caacaggcgg gaatggagat aaaacacatc
     1681 gccagggtac gcttcacgtc cgggggggcg gcggagcaac aaagaaactt ggcgatacgc
     1741 ccaagcgtgc ttggaaagat catcgtaaat gatgagggcg tcacggcctg tttccataaa
     1801 ttcttcaccg atggcggtac cagcatatgg ggcaatgtat tgaagcgcgg ctgattcatc
     1861 agcagaagcc atcacaatga tggtgtgttc catcgcgcca tatttttcga gaataccgag
     1921 agtacgggca acagcagcct tcttctgacc aactgctaca taaatacaaa ccacaccttt
     1981 gcccttttga ttgatgatcg catcgatcgc gatcgcggtc ttaccggtct gacggtcgcc
     2041 aatgatcaat tcgcgctgac cgcggccgat cgggatcatc gagtcgatgg acttgatacc
     2101 ggtctgcacc ggggtatcca catcctgacg ttccaccacg cccggcgcaa tgcgttcgat
     2161 cgggcggaaa cccgttgtgg cgatcggacc cttgccgtcg atcggttcac caagagcgtt
     2221 gaccacacga ccaatcattg catcgcccac aggaactgaa gcaatgcgtc cagtgccgcg
     2281 tacggtcatg ccttcggtga tttcatcgta ctcacccatc acgatcacgc caacactatc
     2341 ctgttccagg ttgaacgccg tccccattac gccgttcgaa aactgtacaa gttcctgcgc
     2401 tcttacattt gcaagaccct gcacacgggc aataccatca cccgcttcaa ctacaacgcc
     2461 gatgtcactg acgttcagtt caggcttaaa gccttcaatt tgtttttgca aatcgcctgt
     2521 gatttgcttg atcaagtctg acattaggta gcctccacca agattcttat taggacaaga
     2581 acccggataa gggcgttatc cgggtatatg tgtaattttt gcccgtttcc cctccccacg
     2641 tcctttcgtg agtaagagta caagcggggc aatgatacct gaaaggggaa tgattgtcta
     2701 gttgcaatcc cctcttacct taagaaatgt ctcataccct tgtcatctac acagcttacg
     2761 ctattattgc ccgaaataat cagaataaac acatcttcag aaaacaagga gacaagatgt
     2821 ctgaaatcaa tcgtgaagaa ggaaaacctc ccagtcgccg tatgaaacgc tactggaaag
     2881 tcgccgttct ggcaaacatt aaagatgaca atcaacccaa acccgaaggg gtaccgccgg
     2941 acgccttcgc cgatttcgac catattgaga ccatagactc gcttcgcgct gcaattgaaa
     3001 ctgatggcca cacaacctac tttatccagg cagatagaga tctacccttc gcgctacgcg
     3061 aaattcaacc cgatatttgc ttcaacattg cagaaggtct tggcggtgat gcgcgcgaag
     3121 ctcaggtgcc cgccctgctc gaaatgctcg gaatccctta cacaggctcc cgcgtactca
     3181 ccaacggaat ctcactggac aaaactctca caaaacgcat ttggcgtgac cgccgacttc
     3241 cagttgcccc atttcaggaa tttaatgcag gcgacgaacc tctgcgcccg gagttgaaat
     3301 ttccactctt tgtcaagcca gctcgtgaag gcacaggcat gggggttgac ctgaaagcca
     3361 tcgtcaataa cgaaaaggaa ctgcgcgagc gtgcccaata tatcgtcaat gtatatcaac
     3421 aacccgctct tgtagaaacc tttttgccag gccgcgaatt cactgttggg atccttggcc
     3481 gcgccgattc aaaactgtat tcccgacatc ccgagtggta tgaaaaagac ggcttccatc
     3541 gttttcccgt ccttgagtta gacagcaccc gctcggtaac accctgggtt tacggacttg
     3601 aagccaaatc aaaagatgta ggtgcagatg gatctcccgg ttacttctgc cccgccgata
     3661 ttgaaccaga gctcgaaaag aaattgaaaa attttgccct gcgtgcccat caacttctaa
     3721 acacactcga tgtttcccgc accgatatac gccttgacga agacggcaac ccgcgcgtca
     3781 tggaaatcaa tacactgcct ggcctgaccc ctgattacag cgatttgtgc ctgcaagcca
     3841 aggcagaagg cattcgctat gaagaccttg tgctcgatat cctctatctc ggcgcaagcc
     3901 gctggggaat gttggagccg cgtgaatatg tgccagacac tccaaagaaa agatagaatc
     3961 acaaaaaaag ccctgcaaaa tttgcagggc tttttacatt aatcatcgtc gccgccctcg
     4021 ccgccttcaa aatcatttcc atcgtgacag gctgtacatg tggcctcctg cacagtggaa
     4081 ggatgacatt gacgacagga agcgccgcca tggaatacac cattcccgcc ttcttcagca
     4141 attggctcgg ggtgttgaat attaaacccg gcaggggtcc atgcaaagat ggtatggcaa
     4201 gtggcacaat tggttccaaa tacgcctgca tggaaggctg ggtcggcatg gcatgataca
     4261 cattcattcg ataaacctgt gaatgagtcg cctttatggc attgttcgca atccacgcct
     4321 gaatgcccgc ccgtcaacgg gaagttggtg cgactgtggt caaaggtcgc gtcgtcccag
     4381 tcatttgtgt tatggcagga tgcgcaatct gtgccgaact taccgttgtg ttcgtcattc
     4441 tgttgatggc aggaatagca atcagtcggc gtacccttga agacgttgtt cacatggcag
     4501 cgctcacaac gtacttccct gtgttcgcca tccagtttga aagcggtcag gttatggtca
     4561 aagtttgcgc tctcccaatt cttcggtgta tgacaggttt cgcattgggt tccaaaacgg
     4621 ccattgtgct catcatcctg ttgatgacag gaataacaat cgctgggcgt gcctttgtac
     4681 acgttgttca catggcactt ttcgcaggct acctcattgt gttcaccttc caacttgaac
     4741 gcagaaaggt tgtggtcaaa ttttgcgggc aaccagcctt cgggggcatg gcaaacacca
     4801 cactcattac catatgcacc accatgtgga tcgtcttcgc gatgacaaga gaagcaatcc
     4861 tgcggcaggg attgtagatc cgcgatgctg cgggcatcga gatggcactc tgtacaatta
     4921 gcctcggcat gctcgccttt taattgaaat ggaaattgat cgtgggtgaa attatcacca
     4981 tatcggtcaa tgccatcatg gcacgccaga caatccgtcc cgaaggacag gacatgcgtc
     5041 tgggtgaaga caatatccat ttgacggtgg cagttatcgc aggagttgga agcgaaggtt
     5101 gtcacatcct cgccgtggca atctttacag ataaacgctt cgcccgttgc cttttgctgg
     5161 tgcccttcca aagaatatcc cagcgcctcg tgcgggaagg tgttctcccc catctcggtc
     5221 agtgaagctg acgcgccacg atgttcaggg tggcaatcac gacaggccag cgtattattt
     5281 ttttgcatga tcgcgccatg taactgtgcc acgtcgtaca tctgagcggc aatatcggta
     5341 tggcatttgg cgcagtgatc tgccattctt gttgcgctcc acggttctgc atggcaggcc
     5401 gaacattccg tgatctgagc atgtgaattg acaccaccca acacctcatc tcctggctct
     5461 gcatttaatg cgccagcagt gaacatttga ctgccgctaa aaaacgcaag ccctgctatg
     5521 gcagcaatgg tgacaagtgc agcaaagatt ccagttcctg ttaaacagcc aagacggtta
     5581 ttggtttgca ctcttccctc ctatcgtagg aacgtggcgt aatagagcgc cgcgcccatg
     5641 tgaataaaag cggagatgaa caatgccata ccgatgggaa tatgaaccgt atgccaaagt
     5701 gacattaacc gccgcgcctg tttcaacgct tcctgtgaaa gcgttccctc aatttcaagc
     5761 ccatcaagcg tacgtgggat gcgggtgtaa atgtaacggc caatgaatcc gctaaaaacg
     5821 atgatgaccg taagcagcgt ggtcgcaccc gcaagcccgt tgaatttcca cgaggtgtgc
     5881 aacaaaacca tgaacggccc caccagacca gtgaaaatgt gaaattgcag ccaggtggac
     5941 attcgcccaa gtgctgcgct tcgactgcgt tttcgcaggc tgtaaagcac ttctgtcatc
     6001 agcatcaaca caaaccctac gatgccgatg gaatggccga ataattcccc cgccgcgggg
     6061 atctcccgcg tccaaatcac aaccgccccg tacaaccccg taatcaacaa acaaaacaca
     6121 aacgccagcc agagttcttt gttacctcga agcatcctct gccctcccga aaaatgtatt
     6181 caactcaagt tgttgaggtt attatttaat tcgttttgta ttttgaccaa aaatccacga
     6241 tcacatctgc aattttggca tcgcgggcga gcagtttatc gcgcatggag gtaatatcca
     6301 ttctgtcgcg tacgatcctt tgcagcggcg ccgaaatggt ctggtcaccc atcactactg
     6361 cgccgatcaa ggtcgtttct ccaaccatca agcggacacg gttcacatca aatccgctct
     6421 gtgccacgat ggcatcgggc aattgccgcc aggtttcact atcgccgcgc gcaattccga
     6481 caatgtcatc atcatggcca tgtcccaccg acccgatgat cgtggtggtc aagcctgcaa
     6541 ggcgggttac gttgaaggga gtattacgaa tataggtctt tctttctcca gtcatattta
     6601 acccagccgt atggccctga tctcgtgcaa ggtgccataa actgtcgatc accgacctgc
     6661 ctgtaacagg gtcaaagacc tgggccacat cgccagcggc aaaaatatcc tcgtgattcg
     6721 tttgcagata ttcattcgtc aaaatgcctc gatctacgga aatgccagca ttgcgggcca
     6781 actcaatacg gggcttgatg ccgatggcat acgccaccat gtcacaacgc atcgcacgcc
     6841 catccttcaa gcggattccg ttcacacggc ttctccctgt gatttcagca agctcggtgt
     6901 gaaaatgcag ggttacacct tcctcctgca aacggcgttc aatgatgcgc gattcaaaat
     6961 catcgagcac attgctccaa tagcgatctc cccgtaaaag ataatgcact ttcaccccac
     7021 gtgataacaa cccctccgcc aattcaagcg cagtaatgcc gccgcccacc accacagcgg
     7081 ttttcccacg cctcgcaaat ttgagtatct tgcgggcatc ttccaaatgg tcgagcttga
     7141 ccacaccttc aaggttggcg ccgggcactg tcaaagggat ggcctgtgcc ccgacagaga
     7201 tgagcagctt atcgtaggaa aggtgcgatt ttccatccaa ctcaatggct ttttctgatg
     7261 gcagtagttt tgtcacccgc gacttatgaa atttaaaatg caaccgcaca tagtcttcac
     7321 gggtacgggg gaacagggct ttatcgtgca attctcccgt caaaaagtac gccagaccgg
     7381 ggcgtgaata aaacccgtgc gggtcatcgc ctaccatcac aatctcacct gacattccat
     7441 tcttgcggat cgcttcaatg gcggccaccc ctgcgacccc tgaaccgatg atgatgtatc
     7501 tcattttcca ccctccgcaa acagatcggt ccgctcgaag cgcaacaccc cacgtgggca
     7561 acgcgccaca cactccccac aagtcaggca ttcgctgtgt ttgatcgcct gcccctccca
     7621 tgcatgagcg cggacatcaa tgccaattgg acagttatac gtgcatatcc cgcattgcac
     7681 acagcgggat gaatcgggca caacatgacc cgctgaaagc gccacgcgct tcgtcgagtc
     7741 tgtcttgaag atattgataa atgacaacct gacctccatg tatcaagtcg tttttccccc
     7801 agaaaagtta cggctttagt atggcaaatt ctatcccctc caccaatacc ccaccagtta
     7861 caaatgtcat tattaaataa aaacagggct gcctcgcggc aaccctgcct gttcgcaaaa
     7921 cttgagccgt caggcttagt cttacgagag cgggactaca ttggcagcct gcaaaccttt
     7981 ggggccctct tcgatggaaa actccacctt ctgttcggcc tccaacttac ggtagccatc
     8041 gctctgaatg gcggtgaaat ggacaaatac atcctcgcca ccatcccgac caataaagcc
     8101 gtagcccttg gtcgaattga accacttgac ggttccaaca aaacgctcag acatctttct
     8161 atactcctgc tgagaaaaaa attgaagccc gttcaccttt ccgctttcaa aggcgacccg
     8221 ggcctgctga gcgcaagatt atcatgccta aaacagcatg tcaagatttt aagaacctcc
     8281 tgcggattga ggcatatcgc gagacaaaat ggaaccacca gggcgaccat ttcacgcgaa
     8341 attctgcact gcgaatcacc ttccgcatct ccgctggacc tgcgaactgg tccagcaaaa
     8401 ggacggatct tcccacctcg aagactgcat gggcatccga accgaccgtg ccagccaggc
     8461 catgaaggcg ggcgaattcc agcgctttgc gattgaaacg cggcaacatg caacgtgaat
     8521 tgtaaacttc gatggcatcc acaaacggaa ttacatccaa caggtctttt tcctgccagc
     8581 cgcccgcgcg caattcatcg aacgggtgcg acacgctgat aaatgccccc tgcgccttta
     8641 aaagcatcaa agtttcttgc ggtgtcagaa aagggggaat ttcctcctgt acaaaagcgg
     8701 ccagaatctc ccctttggtc gtcatgattt cctcgcccac aataaccagt tcagggtcca
     8761 gtgcttgagc cgcgcgtgcg cctgcaatgg aattatggtc ggtgatgacc agacgatcca
     8821 atcccttgcg ccgcgctgcc tgtaccaggt ctttggggtg ggtcagcgaa tccttcgagg
     8881 catgggtatg acagtgaaat tcaagggtca gttttttagg caaagtcata aaggtttaaa
     8941 gatggcctgc accacgccgc aaatataaag gtgcggatca gaaatattaa actcacgttc
     9001 gacgtgtaca gggtttgaac tttgtggctg caggacaatt ctaccgccac ggaccacgat
     9061 ccgtttcaaa gtgacgccat ctccctcgtc attgataatc tctgcagcaa caatatcccc
     9121 gtttctataa ctcgtcaacg gaggcatgtc cccgctctca tcaggctcag cgatgacctg
     9181 gggattcttc ggcaaaaaac gcagcaaaac ataatcgccg cttccgatgt tggccaggtt
     9241 catgctgtca ccggaaacct tgatgaccgc cgtcccatga gttgaactcc gaaaaaagct
     9301 atgaccatcc accgacacga tccgatacgg cttgtcttca ataaagacct gctccacctc
     9361 caccatgccc tctatgggcg tattcaacat cccgcttggg ccaaaatcgc cagcattgat
     9421 cgattcgtgg atggagaaaa gcccgacacg atcagagcgt acctgctgtt cagcatcctc
     9481 cctgctgttg tttgtggcca tctcaccaaa cagggatttg ttttcggggg tttcggcggg
     9541 cagcggcgga atttgatatt tttcagcggc ctgatgaagc gcatcatgca tttccttgca
     9601 ccgtgcatcg taccaatcca acctcgattt tttgttctcc ttatcgcgga tcgctttgaa
     9661 acgtttttcg gcttcgcgcc atgagttgat ggcttccacc tctttattgg gcaaaagcca
     9721 gtccatgcag cctttcatcc atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnatggctt ccacctcttt attgggcaaa agccagtcca tgcagccttt catccataag
     9901 gcaacagcct gatggtgaaa tgaatttaaa tatttggtgg aggcttcgct gaacaatctt
     9961 tgagcctccc taaagttacc catttctgcg acactcaacc cgcactgtag gcaaatctcc
    10021 gcgggctcca acccatcaga agcctgccgc aaacgatcaa ggtcctcgat aatgctccag
    10081 gccttcttca tctcttttct gtcacattcc ctccttaatc gctccaacaa aagattggag
    10141 gggtcactca atgagctttt attaaattta ttcaaccacc cgctgacata tttcaaccag
    10201 cggatggatt tggtctcagt cgtcatcatg gtttctttcc atcctttttc gcgtttttgt
    10261 ctgtgttttg ctcactaccc tcagagacgg aaagcgcctc cagcacctgt gcagtgcgca
    10321 tcaaaataat attgcggata ctgcgatctt tatcttcttt tgccaaatca atatcatcaa
    10381 aggcgtggac aatgctcatc aaaatttctg cctgtgcctc agcatggccg atctcctgat
    10441 acgcgttacg ctgtgcctcg ccataggcgc gtttaacatc agcatttccc atccagccag
    10501 ccttccatgt atcaatgcgt tgttcagaaa cggctttatt gtccacttca aagtgcccga
    10561 tatcacacca tagaagttgg gcacctgcat cctttaattt atcgcgggtg gatgaggttt
    10621 tcaattgggc gctgatctcc ttgcgtgcat caccatcggt atatcgggga gccgtgatct
    10681 gatccagctg atgctttgta atgtaatccg agactgctcc agagatgacg ccctttacca
    10741 tgtcatgcca ggtgttcggt cccgacttcg atacggtgcg gttataagtc aggttgcgga
    10801 tcgcttgaac cgtgaacggg tacgggttgg tcaaacttcg cccggctgca gtattaatct
    10861 cacggtgacc agaccatacg cgatagcgaa actgcacatc tttcactgtc accatgatcc
    10921 catccttggt cactgccgag atcgctggga tcaacccgtg ctggtcatcc agattaaggg
    10981 tgctgacaat cgtttcaaag cgcgacaaaa agaacctgcc cgcagaatac accccggcag
    11041 gcccatgaag cctttcggtc agaatcgcat tgccggggcc aatgaaaaca tagccagggc
    11101 cgccgatctg gtcaatgcga ttttttccat cacccacaac gtaattttct gagattttga
    11161 tcttcgggta tttgattccg caaaatgatg aaagcagata cagaaccgcc aacacataat
    11221 gcttgatatt gaatacatcc tgaacatatc gtgcccctga caaaaaagca ctgatcaatg
    11281 ccgccaaagg gaggatcaag aaacgtgaaa tgggctcgcc agtcaaaagc ggccgtacat
    11341 agacaaactc gaaccaggag agcggcccga taaatatggc aaacacgatc acataccaaa
    11401 cctgtttcag aggcttcgcc cgttcctcct catatcgttc aacaaggatg taagcgctca
    11461 aaagaatgga agcagccaac caaatcatcg ggaaggcaat atcattccaa attcgcaaaa
    11521 gtgtgagttt atccgctgaa aacccggccg caataaaaac agagaccgca gccgttgcaa
    11581 aaagcaaaaa taacccgcga agtcgtgccc agatcaaatc tcccaccaga aggttttgaa
    11641 ggatgcgcga gacccgtgtt ttggcagaac ggaaaatcat catggcgtac cttcttcgcc
    11701 aattacaggg tttgattcgt ctgatttttt attttccggc aaaagccaat gccgcaaatt
    11761 ggaaagcatc tgaaccgtat cagcgggcaa caaacgctgt gttgccgggt tgatcgctgc
    11821 cgcctccaaa gcctgataca aacgcaacgc caaagcctct tccgtatatt gattctcgtg
    11881 gaaaatccgc gaaagggaat agatcatatc ctgctgggtc cgcgaacgtt catggttatg
    11941 aattcgcatg gcgcgcagtt catggttggc cagagtcttt tgagcctcct gctgccaatg
    12001 tgcccgccaa ttttcaaaaa gttgagcacg gacatcttca ttaactggaa caaggtcacc
    12061 aaacccggca ctaaggacct taatgccacg atctcgaagg atcgcagggc ctttaaatct
    12121 caaaggtggt gaaaacttca aatcggcctc gttcacaaac tgcgcgtctt tgatcggggt
    12181 ttcatccgca agcatcacaa cctgataccc caacatcccc tggttggtca ccatccgccc
    12241 gaatttcctt ttgaagtcac ccattgggaa ctcattaggt tcttctggtt tgtaaaggtc
    12301 atcgtaattc tcgtgcgcca actgttcccg aaaaatatca gcagccacat atccaggcag
    12361 ttccgtccag tcgatcaact gggattcgcc agtagcactg cgggcacgcg aatacacagc
    12421 agaaaacacc cgctcttttt caaaaacaaa ttgtgaagcc gctccaacct tcacaatgcg
    12481 gatgtcggta tcactccact caaattcttt ttccgtaaag gcatgctcca gttcgttcat
    12541 atcctgttca ttgatggtat ccacgggctg aaatttcaca acctgatcgc ctgggcgggg
    12601 ttcaggcctg tccaccaggt cttcagcaga tggtgccgta atctgcactg cccgccagcc
    12661 caaatcggtc cgcgtaactt tcaaaatatc ctccgcctgc ccaagagtga aggttacgtt
    12721 cacattggtt gtaatctcaa ttccatcccg cgtgcaggct ttcactcctt gcacagatct
    12781 actctgcttt ctcagatcca caaaccccac gatcttttcc ccggattcgg taaagacgac
    12841 accgggaccg aatgccttca caaaagtgac attgacattc ttcccagcga ctcccaactt
    12901 agccgtaatc tgcttccaca tcccaggccg ttgcagccct tgaacatcct cctcctcagc
    12961 ttcaaaatgc acctcctgcc gcaacgaatc aatggaccca gtctgttgtt ccaaaacgat
    13021 cgcactatac agatccacca aagccacacc agccttatgg ctgtctaatt catgttcctc
    13081 catgttcggc tttccttctt ttacaaagat cgccggtcca tgccattttt tcccagccaa
    13141 agaacggatc aacccggtaa acgctttttg agtttgcacc aaaccgcgta caggcaaaat
    13201 aaacggagat acccaaaaca gcacaacaaa aaatatgaga acaaaaacgg aaatattggc
    13261 aaaaagcatc gaactggcga ggctagcctc tggagaaaca tacaccgcat cagctgcgcg
    13321 caaaatataa acatatcccc atgaaatgac caactgccag acaagtttta tttcgatgaa
    13381 gaaagacaaa ccagacagag atatgaccaa aacggcagtt ccaagaatcg aaagcctcgc
    13441 ctgccgtgag atccgggtat ccgcgggcat gatattcatc cgtcccccat tgagggattc
    13501 aagactccca ataaatcatc aacctccagg ttgctatatt tgatccccag atcgcgtcgc
    13561 atattggcaa caagagcaaa cagcttctcg ttggttacgc gttgggcttt cccatgtaca
    13621 gccacacttt cgcgccaatc acgaaaagca cggaccactt catccgaacc gatcaaaacg
    13681 atctgtgagg caatggctgt ttccgcctcc tgcctgtgat ccgtttcaaa ggtattaaga
    13741 gaaggggctt gcagcagttt tgcaaactcc gtatagacac cccacttctt gtcgttaaat
    13801 cgtttcaaat attcctgttc gagatctgct tttgcctgtc gccaaaaccc catgaaagta
    13861 aatgcacctg ttgccagggc aattacgaac gcaaccacga tctctgccgc taacccaccg
    13921 atgttttcca tatgaagact ccccgataac tgattgaaaa tgtggataga tacgatgctc
    13981 aaaaatcagt atatacacgc aacgtcttca ttgtcaagag gggggatttt tggatgcaat
    14041 tcaccatctc acaaaactca actccaaata ggcgaagtgt gtcataatta tggacagtcc
    14101 gtaacttttt ccgcacacca gcgactatat ataagatacc tgtatttcgc caacataaag
    14161 gagacaagca tgaaaacaag aaatttcttg attcgcatcg gtctggcact attagttgct
    14221 gccctattgg gagcaggggc aggtctacta gcacgtgaaa aggtggatgc cgcagagggg
    14281 acatacaaaa tcctggcttg gaatgacctg ggaatgcact gctataaccg tgactttgca
    14341 gacctcgcag tattaccccc atacaacacc ttatgggttc aggtgatcaa agctggcgat
    14401 ccgccgactc tggtcaccag cggtgttacc gtggaatatt tctacgaaga caatacatac
    14461 tcggtcggga agaccaattt ctggtcctac tcgaaagacc tgttcggggt taacctaccg
    14521 cctaatgtcg gtctgaaagg aaaaggtctt tctgggacca tggatttcag cggcgaccat
    14581 tttgtagcag aaggtatccc tgttaccgaa ttcagtgaca gcgcccctac caccagccaa
    14641 ccctatcagc tcgccactgt catcgtaaaa gattcagcca ccggcgcaga actggcacgg
    14701 acaagggtgg tcacacctgt ttcttcagaa atgcgctgtg ataagtgcca cagtgacggc
    14761 ggtgaagcca gccccgacaa tccaacggga aaggtcgaaa ccaatatttt gcagttgcat
    14821 gatgaagaag aaggcacaaa tttaatggcc aatcgcccgg tgttgtgtgc agattgccat
    14881 gcatccaatg cacttggcgc ccctggacaa tcgggaatac ccaacttatc aaatgcaatg
    14941 cacaacaagc atgaggatga atcgcccaat acgctgaatg gctgctacaa ctgccatcct
    15001 ggcccggaga caaaatgtct gcgcgacaca atgtcacaag aagggatgac atgcattgac
    15061 tgtcatggcg gcatgtctga tgtgaaaaac aaccccgatc cctggctgaa tgagccgcgc
    15121 tgcgacacat gccacgacag tgggcaatac gaccaaaatc atccgctcta ccgcttctcc
    15181 accgaacacg gaggcttgta ctgtgaagcc tgtcatgaca gcacacatgc ggtcgcacca
    15241 agtcgtgaag aaaatgatgc gatcaaattt aatatcctgc aaggcaagaa tggcccggta
    15301 gaccaatgta cggtttgcca tacaaccact ccatcaacag gctcagggcc acatgggctc
    15361 acctcttcag gagcgctatt cgaagatgta ccctctacat attgggcgcg ggatttcatt
    15421 gaaagactgt ataacgcagg catcaccagt ggatgcagta cctccccgct gatgtattgc
    15481 ccggacgcaa cggtcacacg cgcacagatg gcaatcttta ttctgcgcgg catgcatggg
    15541 agcgcctata tcccacccac tgcatcggga actgtcttca gcgatgttcc agcaggttcc
    15601 tttgccgcag attggatcga acaactcgca gccgaaggtg tgaccgcggg ttgtggcgga
    15661 agcaattact gcccggacgc caccattaca cgcgctcaaa tggcgatctt ccttttgcgt
    15721 ggcgaacatg gaaatgcata cgcccctcca gccgcaaccg gcaccgtctt cggtgatgtg
    15781 ccgcttggtt cattcgccga cgcctggatc gaacaactcg cagccgaagg ggtcacaagt
    15841 ggatgcggcg gtggaaatta ctgccccgac aactcagtga cacgtgctga aatggctgta
    15901 ttccttgtaa gagcattcag tctgccataa cagacattca acacagaaag cgccgcgatt
    15961 ttctcgcggc gcttttaatt tcaagatcac cctccaaaca aatttgtgaa gcaaaaaata
    16021 aaaaatatga cattggtatt attgaacagc tcatgaaaag ttgtatattg aaaatgtgtt
    16081 gagtttatat acaacctggg agggttgaca tgaataccaa aagattgttt tcccgcataa
    16141 tcaccatcct aattgtcgtt atattctttt tatcaggcac tgcgccaacg ctggctgcac
    16201 ctgctcccat tccgctttac gcagacgatc caacaccgcc cgctcaaacc gtcaaactca
    16261 tcttcatcca ccattccaca ggtgagaatt ggctggccga tggatacggt aatcttggga
    16321 tcgctctgga acagaacaac tattttgtca gcggcactaa ctacggctgg ggaccaaatg
    16381 ccatcggtga ccgaacagac atcccgaatt ggaccgaatg gttccgttcc ggagatacag
    16441 agacgtacat gaacgccctg ttcaacgaaa gcggacaaaa ctcctccttc acccgaaacc
    16501 tgaccgaccc aggcggagag aacgagatca tccttttcaa atcctgtttt ccaaattccg
    16561 cgcttgaagg cagcccaacc gacccgccat cacccgatgg ctggctgaca gtaggacatg
    16621 ccaaatatgt atacaacgag atcctgcagt actttgcatc ccgtcccgac aaactattta
    16681 tcgtcatcac tgccccacca ttgcaggatg gcacctacgc cgaaaatgcc cgcgccttca
    16741 acgaatggct gatgaacgac tggttgaccg aaaacggtta taccctgccc aatgtggcta
    16801 tctttgattt ctacaacgtg ctgaccgggc cagacaatca tcaccggtac atcaatggca
    16861 ccatcgaaca tgtctttacg ccaggcatga acacatccta ctatccatca tctgctgacg
    16921 acgaccatcc cagcgcagca ggcagtcaaa aagcgacaga tgaattcgtt tccctgatca
    16981 atatcttcta caaccgctgg cgaaattcaa cttcatccac cttcaccgat gtaccttcaa
    17041 catattgggc agccgcctat attgaaagac tctacaacgc cggtatcacc agcggatgca
    17101 gcacctcgcc catgatgtat tgcccgcttg cccctgtcac ccgcgcacag atgtccatct
    17161 tcatcctgcg cggcatgcat ggaagcgcct atatcccacc cgcggctaca ggcacagtat
    17221 tcagcgatgt acccgctgat gccttcgcag ccgcatggat cgaacagttt gccagtgaag
    17281 gcatcaccag tggatgtgga agcgggaatt actgccccga tgccaacgtc acccgcgcac
    17341 aaatggcgat cttcctgttg aaaggtgagc atggctccag ttatgttccc cccacagcaa
    17401 cgggtatctt caccgatgtc cccatcggct cctttgccgc agactggatc gaacaactcg
    17461 ccaacgaagg catcaccagc gggtgcggtg gcggaaacta ctgccccgat gccgatgtca
    17521 cccgtgacca aatggccgtc ttccttgtca gggcattcag cctgccgtaa tttcttgacg
    17581 catgcctttt gcggtggtat ccttgcgcgc aatgttcagc cagattgtag ttgacaccac
    17641 taccaatacc actcgtacca ataatacagg gacggggcat tggtggttgt caggtgaaca
    17701 gcaacaagtt catactcact aggtacacca aacgccccga aaaaaattcg gggcgttttt
    17761 cttaacatca aaaaggagaa gcaatcatgt ccatttcaaa cgccacaccc gtcctcgccc
    17821 ttgacgagaa aaacctcatc cctgtcagcg atcacctcaa acagatggcc gatattcccc
    17881 cttcacgcat gttcctcatc aacaaatcgt tgaaggtcta tgtggaaaag aacgcaggcg
    17941 cgaaaatatt cgacgcttca caaggcgatg gaggcgcttc actccccggc acaccgaaag
    18001 cgattttgga acgcgcgctt caacttcaac tggaacatgg tacggcttac gatatgccat
    18061 tcggcaccga agcatatcgc aaggcggtca tcgaacaata ttggaaatta gactcatcct
    18121 ctggttgggg gcctgcgaac gtactcggca ccgcaggcgg gcgcgatgca ctggtcaagg
    18181 tctatggagc catgttggcc ctcgggcatg gacggcaggg tgatctcatt atggtctccc
    18241 gcgtcccatg gatctcctac aattgggggc catacggtgt cggggcgaac gtgctctggg
    18301 ctcccggcga cccagcccaa gggtgggctt attcggaaga cgccatccgc gaaagcgtca
    18361 agttcgccga atccaaaggc agaaagatcg caggcattgt catcaccaat ccagataacc
    18421 ccaccgggtt gacaattgcg gttgaaaaac aggtttcact cgccaaagcc gccctcgaag
    18481 ccggtgtggc cttcgtgctc tttgactgga tgtatcacta cgtcacagac gaatcgccga
    18541 tggatctgaa ttccttcctc aagaacttca cccccgaaga acgcaaacgc ctgatgtttc
    18601 tcgatggcat caccaaatca ctcggcgggt cgaacatccg caactgccat ttgatcgccg
    18661 acgaagctgt gatcaaattc atcatggccc gcgcttcaca tggggttatt ccttccttct
    18721 actcgctggc cgttgccatg gccgcctacg aaatgggatt caccgaagcc gccaagacca
    18781 tcatcgaacc aaccaatgcc agccgcgccg tattaaagaa attgctcgcc gaaagcggaa
    18841 tgcagcacat tattggcaag ggctattacg ccttcatgaa tgtcggtgaa tttatcaaag
    18901 ccaagggttg ggccgacagc gaaccgctcg gccaatacct tgccgaaaat catggcgttg
    18961 ccatcgtccc tggcgccttc ttctccccct ttggtggaga ttggatccgc ttctcgtatg
    19021 ccactcccac cgaaagaacc gaaggcgcat tcaaacgatt ggtggaaggg ctggagagtt
    19081 tgaaaaaata aaaagggttg aaagttgcaa gtggaaggtt taaagtaaaa acacttccac
    19141 tttccacttc ccatttacaa attcgcaccc aattaggatc atctcatgcc ccaacaattt
    19201 gccgataaat atctcctcgc cgtcgaacag gccatcaagg aaaagccgca aggtggtctc
    19261 gacggtttcg agcaggaatg gaatttgctc gatgaagaac tgcgcccgtt actaaccgtt
    19321 ggcgcaggac ccagccaaca ctcgtttgtg gattatctgc gcgccgagtg tgttcctcac
    19381 tggcatcaac aattcagcca gctcgaagtc tttcactgga tggtggagtg ggccacacgt
    19441 ccctactaca ccccacgcgg agcgatgtac gaagcccgcc tgatggaagc cactttgatg
    19501 aatgccctgc accgcgcagg ggtcaacttt ggagagcgcc tgcactactg gcatggcaac
    19561 ctgctcttcc ttaccgacat cgggcatcaa tccatcccgg gaaactggtc gcttgccaaa
    19621 cgccgctatc tcgaaaaatg cgtggaccta tacggtgaca ctctcgccac cacaggcatc
    19681 cacaccaaca tgtcactacc cgatccgctc tttgcatggg attttatgca cctttccccc
    19741 agcgaacgcg gcgatcaaca ccttgatgaa ttcaaatcgg agttctacat cactgctaca
    19801 cggctgctcc gtgccttcgc ctctctcttc attgcaacca gcgcatccac accgatgcag
    19861 gcacaggtca aggacggacg cgcagcggtc atcctgaccg agtttgattc gattcgaaac
    19921 ctcactttcc caaatccacg tgagatcgat ctccccgatc tatatcgctc atacaacgac
    19981 tacctgcaaa tttcatacga cctcgtgcgg cgcggtgtgc gtttcggcaa caacaactgg
    20041 actcccgtac gcgcgcgcag tttctccgaa cctgtcgagc ggatcatctc cacgaccagc
    20101 gatcaactga gttcactcta tgcacgcggc ttattcgccg ccgggcaatc aaccccgccc
    20161 gaagaaatgg cgcgacaagt ggaacggcag aatcttatgg cgcgaatcaa cctgcccatg
    20221 ggacgcgtcg aagtccgtgt ggatgagggc ggtcacagcc tggacctcga tattgccaac
    20281 atgaccttca ggcatttact gctcctgaaa atttactccg acccgcagtt tgcacgtgga
    20341 ttccgttacg accgtgaaga tatcacccgt gcccgcgcca atgaaaacat ggccgccaaa
    20401 catggtctgc gtgctgagat cgaaaacccg ctgaccggca agcccattac cgtgcgcgag
    20461 tttctcaaat ggacactgaa cgaactcaaa ccgctggcgc agggattaaa catgtgggac
    20521 gacctgcatc cgcttgtgga aatgtcggaa ggtggacgca acacttccga aaaaattcgt
    20581 gcgcgtctca aaatggagat cggtgacgat aacgaagtac cgctcccgct cttgaaggaa
    20641 ttcttctacg aacatgaagc acaggtgaaa gccgatgtgg aaaccatttg cgccaaccat
    20701 gcttcactgg gcgccgattc tgccaagatc gccgagttca ttcaacgcag ccgcgacacg
    20761 gttcgcagtt ccgaaattcc gaatgcacct gtgcaattcc gtccgcgcgc gcaggctgtg
    20821 attgaaatat cgtatccaaa caaaaccgcc gagatccttg atcttgccca gcaattgatt
    20881 cgcatcccct ctgtcactgc ctgcccggtg gagcgtttcg aagaagttca ccgtgctggt
    20941 tcattaattg acgattacct gcgcaatgca ggtctggatg tgaaatactt cgacgggaaa
    21001 tatccagcca tttacgcgac cttccccggc gcatccaaac cgaacccgat cctgctgact
    21061 ggtcactttg atgtggtcga acccgaaccc gacgactcgc aattcaatcc acgtatcgaa
    21121 ggcgattacc tcttcggccg cggcgcagcg gatatgaaaa ccgttgttgc cacatatttg
    21181 gtttggatga aggatatgtg caagagcgca tcgcgcccca acattgcctt gatgctggtt
    21241 ggcaatgaag aaaacggcga agccgaagcc tggggcacgc cgcatgtttt gaaagagcta
    21301 aacctcacac cctctctctt cattgctggc gagcgcaccg gcgaaaaggg aaatgaactc
    21361 ttcggcgaaa tctgcgtgga gaatcgcggc gtgatgcgct ttgacgtgat cgcgcgcggt
    21421 gcaaaaggtc acagcggcgt ggcagggact ggcgatttga gcgagaagct cattgctgca
    21481 cgctctgcac tgaatgagat cttcgcaagc catctcacac tcaaagcggc cgatggctgg
    21541 caatcacaag ccaaattccc gtttatcacg gtcggcacac cgggcgtgta caacgtcact
    21601 gcggcagaag ggatcctggg tgtggagatc cgccccattc cacaggatga tgtgactggt
    21661 ttacgcttga aagttgaaag ttattgtgct gaaaatggtc tcgaagcaag ttttaacgtg
    21721 atggaaaacg gcgtggcttg cgacccgaat aacccagcgt tgaaagcgct catcgaagca
    21781 gtgagacagg cttcgggtgg agaagaaccg cgcatcggaa agaagttacc cggcaccagt
    21841 gcaagattcg ctcctggcgg tcaagccgtg gtgtggggcc aatcgggcgt gggtccgcat
    21901 gcgaagaacg aggcgcatta catcccaagc atcgaaccgt attacaactc gctgaatgaa
    21961 ctggcgaagt tgtggaaata aaaacaaagg cgaccttgaa caggtcgcct ttgtttttat
    22021 ggcaatggtt ttataagcaa aagatttggc tacccatctg cataattggc aacgatcaac
    22081 ttcagtagct gctcgttgaa cctgcgggcg tcagcgccga gttgactgaa aaattttcgg
    22141 tcaattgctt ccactgcagg cacggcctgc ccggccaatt tgcgcccctg tgcagtaatg
    22201 gacaatgcct ttgcccgcgt atccgtcggg tgcggctttc gcaggatcaa ccctttttcc
    22261 tccaacgtgc gcaacacatt ggaagtcatc atcacatcca ttttggcgtg attggcaaga
    22321 tccacctgtg taagaggttt atcctcattc gccaaccaca tgagagaagc cagtaaaaca
    22381 aattgcgcat gggtgaggtc gaatggtttc aaccccgcat tgatctgtct ctgccacatg
    22441 ctggaaacct gccaaagcga aaaccctggg ctgtcatcgg cattatcaaa ttgaaaaacg
    22501 tcatcaggca ttgattttct ccgtcaacat ctgactggcg atcaagtcaa agtcaatttt
    22561 tgggatttcg aagaacccaa agcgaaacat gtagccccaa ctcgttttat ctttgatgaa
    22621 tgtaagagct ggaatcaacg gttggatggg aacttcaaca cattccttgt attttacatc
    22681 acgacgatag ggaacaaatc cgccgcccat atcaaattga taaacagttt cacccgtcac
    22741 ttcaccgatg gcggtaaatg tctgtaattt ctcgttgcct tcaaattcaa cttttggtga
    22801 ataatacaca atgccatcgc ccactcgcat tcgcttaagc ggagccgcct tgccgtgatt
    22861 ggcttgcgca attccccaac gcacgccgtt catcacatga ttctttgaga caacgatcaa
    22921 ccaatagcgc gcctcgctca tttggactcc tttgcaagtc tgaccaacct gccgatcgtc
    22981 tcaggcaaat ctctggcggt atctttccca atcacctgcg cgaacagaaa actcagcgga
    23041 cccgagatcg tcacgcgatg agtcacatgc aagccatctt tttcttcttt cagttcgtga
    23101 gcaaattcca ttttcgccag cggcagactg gtcacatcca caaagcggct caaaggctgg
    23161 caatcaatga tcactgcctt tgcttcgggt ccacccactg gcttgagcgt gtattttgtg
    23221 cccgctttga aatcgccctc aatgcggcac cactccacac ctttatccca ttttgaccaa
    23281 ttctccacat ctgcccaaac cttccaaacc gcctccttgg aagcgtctgt cacgagtgta
    23341 tgttcagttg tccacataat ttctctcctt gatttatata tacacttatt atatatatgt
    23401 atattatttt gtcaagga
//
LOCUS       DAOQLC010000068        23088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_759_length_23088_cov_24.724656, whole genome shotgun sequence.
ACCESSION   DAOQLC010000068 DAOQLC010000000
VERSION     DAOQLC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 23088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_759_length_23088_cov_24.724656"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PL000_12770"
     CDS             complement(<1..509)
                     /locus_tag="PL000_12770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HNC89820.1"
                     /translation="MKTALLVIDIQKDYFPGGKYELVNPLAAAKNAYMLLQCFREHGG
                     HHVHIQHISLEPDATFFIKGDSGSDIHDSAAHFEGEPIVYKHEPNSFLNTNLLDLLKG
                     WGVERVVITGMMSHMCVDATARAASDLGFKVIVAEDACATRDLQFDGTTVPADHVHKA
                     FMSALQSYG"
     gene            complement(581..2110)
                     /locus_tag="PL000_12775"
     CDS             complement(581..2110)
                     /locus_tag="PL000_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNC89821.1"
                     /translation="MDVRFHFSMIFSLLITYFIFRPIDLRSTVLALLWLTGFVLCILL
                     HELGHALAAKLVGVDVKSIIIWLLGGLTSLERKPERPFHNLVIAAAGPMVNMLLGFIF
                     VAGYILLHIFAPRGLEPELFIWVQTFIELCFSLALLNVILVVFNLLPIYPLDGGNIMH
                     AVLDGLFGRSSADLITMLVSIPVLLGLVVLGLYTRDYLLLASCVLIALAIGTLNRSVL
                     RWVNLGVNYLFKRSGYYYLQGDYERAAQLYTREIEREPEQPAHYLARAACLLNVMQKE
                     RALADIDRALKLNPKNYIALELRGELYSMDKDYDSALEYFARAQELSPNWAVPHFDRA
                     SILMDKQEFGPALAELNQSISLMSQFTLFYVVRSLAHFKLGNVEAAHKDQDAALRLSE
                     KDALTVSPVNMTLYEGYLDWAEEYYERAISKLPRSFLAYQGRADAYRTNKEFEKAIAD
                     YTHAIQLAPHEADLYLGRGKCHQALNDPEKAAADFRLASEKADKLHLKRQAEELLKSV
                     V"
     gene            complement(2211..2834)
                     /locus_tag="PL000_12780"
     CDS             complement(2211..2834)
                     /locus_tag="PL000_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HNC89822.1"
                     /translation="MAKKKFYVVWKGRKTGVFTTWADCEKQVKGVVGAQFKAFESEAE
                     ADAAFLKQYEDYKGKSSTHGKWKMASTKPILPSICVDAACSGSTGKLEYRGVNTETGE
                     EIFYAGPYAEGTNNVGEFLAIVHALTWQAKHNMHVPIYSDSENAIAWVATGICKTNLK
                     HTPKNAVLFAIIRSAENWLAENELPEDKIIKWDTGLWGENPADFGRK"
     gene            3155..3994
                     /locus_tag="PL000_12785"
     CDS             3155..3994
                     /locus_tag="PL000_12785"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HNC89823.1"
                     /translation="MSYAHPEYLVDTEWVANHLNDANVRIIESDEDPLLYAIGHIPGS
                     VQVDWFSTLQHPLRRDFVTKEQFEEVASKLGITNDTTVVFYGDKSNWFACYALWLFQY
                     YGHANVKIMNGGRIKWEQEKRPLVKEVPSYARTTYSAKEADKSIRAFRDDIFKQIEGK
                     RPMVDVRSPKEYTGELTHMPNYPQEGATRGGHIPGAVSIPWSQAVNEADATFKTTEEL
                     VALYEGKNIKPDGEIIAYCRIGERSSLTWFVLKYLLGYKKVKNYDGSWTEWGNLVDAP
                     IEK"
     gene            4041..4469
                     /locus_tag="PL000_12790"
     CDS             4041..4469
                     /locus_tag="PL000_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HNC89824.1"
                     /translation="MTLPTKLQEIVDDIASMSREEKLETLIAYAESMPDLPERFKDER
                     SKMEPVPECMTPVFIIAEKLESGGIFFHLDIPPQSPTVRGLASILTNGLNGCLLEEIL
                     SVPADFYIPMNLQEAVSQQRINGFIGVLAHMKQAAVKASS"
     gene            complement(4595..5008)
                     /locus_tag="PL000_12795"
     CDS             complement(4595..5008)
                     /locus_tag="PL000_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC89825.1"
                     /translation="MPHLVRDWMSSPVVVVDPDSSVSHAMTLMRRRNIHSVVVDVSEK
                     NPSYGIVTTTDIRDKIVAVNRNPAEITVREIMSGPIITGRAEWTLMECSQLMQKHKFH
                     HLPIADEHGSLIGLISATDIFMSVEEQGWEEEKKD"
     gene            complement(5042..5968)
                     /locus_tag="PL000_12800"
     CDS             complement(5042..5968)
                     /locus_tag="PL000_12800"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HNC89826.1"
                     /translation="MNRNHLALVLILTLATLACGLAAPASNAPTPAPVQETVPAPTES
                     APSLTVDQLKNAQYQLGARDDHAVIQLTDGKYQQGTDATTLDFAYIGLTQFSSVGDLT
                     GDGVNEIAAIFLENYGGTGNFGLLAIYSNVNGLPVFLTSTFIDDRPMINSMSIENGEV
                     VLDAIVHGFQDGGCCPTLPTTRRYTLVNNQLRMVNYTTATPEGAKRIVEIAAPANGAE
                     ASGSVQVSGTVSIAPFENNLSYFIFDEAGNQLAAGPVMVTAPDFGAPGTFNETFSLEG
                     IPSGTTVYLEIQDISAADGSWLAMDAVKLVIK"
     gene            complement(5981..>7041)
                     /locus_tag="PL000_12805"
     CDS             complement(5981..>7041)
                     /locus_tag="PL000_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89827.1"
                     /translation="QPMKKIPSFLFLLTLLTACSTPTLAIPPTLPPVENTPLPTQALQ
                     FTPIPTLTFTPVPTEGFPTFVAPTSTPDPSTRPNTIQFPANGTYVDINDFIAINTSKT
                     YTVNAMKGQIMSVSVLPQSADNNWTYIPLQIKGADGSVLCPQTESDCTAWRGVLPATQ
                     DYFITLSPATDVIQFMLRVAINPPGKSEQYFQYSNPANGVALTYSDYFAPVFPVFSNY
                     KIVPELALYMNDQETYVKTNLSEAYFFLGSSADPQMVATCTDSNPNGGAIEQIVGNEV
                     INGFTFVHSTSAGAGAGNLYEQEIYRMAYNNICYEVIYHMHSANIGNFPPDTVTEFDR
                     NAIMNKMYTVFSTLTISK"
     assembly_gap    7042..7141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7222..8433)
                     /locus_tag="PL000_12810"
     CDS             complement(7222..8433)
                     /locus_tag="PL000_12810"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC89828.1"
                     /translation="MNTPVIIDAIRTPIGALGGILSSVRPDDMAAHVIKAILERNQID
                     PALIEEVFLGCANQAGEDNRNVARMAALLSGLPLEVGGVTVNRLCASGLNAINMASRA
                     IKAGEGDIYIAGGVESMSRAPYSLPKAEAGFSFGNLTAYDTALGWRYPNPKMKEMYGT
                     DSMGETAENIAAERPHLTREKQDEFSVRSHQLAVKAIDTGRFKQEIVPISIPQKKGDP
                     KLVDTDERPRRDTTMESLAKLKPAFKKEGGTVTAGNSSGLNDGAAALLIMSEEKAKAL
                     NLKPLARIVTSASAGVPPRVMGYGPVPATKKALQRAGLRIQDISLMELNEAFAVQSLA
                     VIEDLGIDPDLVNVNGGAIAIGHPLGCSGARLVTTLVHEMKNRGNAKYGVATLCVGVG
                     QGEATVIEYLG"
     gene            8581..9150
                     /locus_tag="PL000_12815"
     CDS             8581..9150
                     /locus_tag="PL000_12815"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC89829.1"
                     /translation="MPLEHAILAFLEYNPMSGYDLKKLFDVSVAHFWSATQSHIYKSL
                     EGLEKKGWAEAQIIPQEGKPNRKEYQITAEGRIELRRWLVTPIPLGPVREACLIQIFF
                     SHFSTNEEIAGLFETRMQEIREHLHILKTVAQTAIDENAKQVGVERARQLWQITLDYG
                     IDYYEFELAWHEKTLKKIHNLPPLTLPNS"
     gene            9235..11310
                     /locus_tag="PL000_12820"
     CDS             9235..11310
                     /locus_tag="PL000_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC89830.1"
                     /translation="MFQVFVGFIPWIIYWSFSGPGLWTIAILGGATAAGGVVAWRWLK
                     RRNVKTMEIVTLGYFIIHAILTLGFGSSFLKTYGPIANSLVLALMSVSTLAMKNPFTY
                     QYAKEDWDKSYWNDPAFIRINEIITSVWAGIFTVNTLLGVLAVAIPAQKLIWAVIVPN
                     VGVVVGIIFSSRFPAYYSRKSVQARLDAWDPYKWSAPKFDKRPAATNEHDVIVIGSGI
                     GGLSAAALLAKRGLKVAVFEQHYLPGGFCTAWDRNVRIPGQPIESRERWTYIFDAGVH
                     DFSGLGERGGIRSLLRMLDIENAIEWKLNEQEYFLGETHFKVPHDANEFIQQLGERFP
                     SEAQNIRAFFDEMLGVYRDMYADMEKLGGAPRTPDNVDDLLAYPRTHPHSYKWMDRPF
                     IEMLDTFFKDARVKELLCALTGYLSDDPKSLSVGNMAPIFGYHFDGGYYPVGGSQAFP
                     NALVDAIEKHNGKVYLRTPVKRILTEKGRAVGIELKDGTTHHAKALISNADTHKTFFE
                     LLGREHLPADFTNYIDSLKPSTTAFIVFLGLDITPNLAPITMLDDIGIMIPSNVDPSL
                     APPKHASVTLLKLLPESEFAKWDRKAEGYNQRKRAYADQMIASAAEVIPGLQQHITYR
                     QEGTPATFARYAWTTNGSIYGPAMGQKRLTVKTPVKNLYVAGSGVMGGGVEAAVIAGI
                     HAANEIYKN"
     gene            11365..11823
                     /locus_tag="PL000_12825"
     CDS             11365..11823
                     /locus_tag="PL000_12825"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HNC89831.1"
                     /translation="MIYAIAKWIHILCAIISLGANLTYFPWFMTVPKTRETLVYTLKT
                     IRLLDNWIANPAYILAYVTGEIMMRVGGQIHYSTPWMTVALILYVVISILGLFVYAPM
                     LKKQQQLAETKGADDPDYQRVSRNGIILGTVIVIITIGITYLMATKPQLW"
     gene            11840..12553
                     /locus_tag="PL000_12830"
     CDS             11840..12553
                     /locus_tag="PL000_12830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC89832.1"
                     /translation="MILFLTLLLVILAGAGIFALRPVVRNIQTVFHTDKTVDYQIKFQ
                     SLQLILAAVVLALTYLLNPKNFQTFFQPGNVNANISKIPWLGVTGQETWLEIALTLGF
                     FITVATGIFMFLQLKKAGVDYRFLLFSLLWSIPFSVANAFSEEAIFRMGIVSPLFGVL
                     STSVILLLSGVIFGAPHYFGQPSGVVGAVMAGFLGWLLAMSLVETQGFLIAWAIHFAQ
                     DVVIITSMILMGRKQLSNR"
     gene            12601..13077
                     /locus_tag="PL000_12835"
     CDS             12601..13077
                     /locus_tag="PL000_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89833.1"
                     /translation="MSKITQTNGKIEITETMSTGIRVFLFLLGLLPWLAPYELLIKPG
                     WTGFHISTLVFLSISLGAIAVSIGFIGAAIFGMNQTATFDLTARTITHRYETTVNALR
                     TKRYSFNDISKSEVREHNWDSGPNTYSLEIHFNDKHKILCGNFPSRKEAEEILKYL"
     gene            13085..13945
                     /locus_tag="PL000_12840"
     CDS             13085..13945
                     /locus_tag="PL000_12840"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC89834.1"
                     /translation="MSSIDKTILVIGGTGMLGKPVAQQLKADGFNVRLLVRNPEKAKK
                     IFGDGYELIQGDVDNVASLSAALTGVDGVHISLKGGPTEADFERMDHFAVRDISKIAK
                     EKNVGRVTLISAYAVSEEKADTPESRSKVRGEAALKSSGVPYTIFRASWFMETLPLFV
                     QGKSISLIGKQIHPLHWIAAEDYARMVSKSYQTEETLNKELYIFGPEAYTMGEAMKIY
                     ADHAGVKVSPMSTQMLAILGTLTLNTEWKGMSVLMKHYENWGEDGSPDEANRLLGAPQ
                     VTLREWLARS"
     gene            14040..14651
                     /locus_tag="PL000_12845"
     CDS             14040..14651
                     /locus_tag="PL000_12845"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC89835.1"
                     /translation="MSPRTYKASEQTKTNILQKAVELFNEHGTAGISMNSLAETLGMS
                     AGNLQYHYRNKEELLRALLELMFKEFDAIYPSSASTFTLDTLRNLMRLNFELSWKYRF
                     FFRELGALLRNDESLAIRFREIQEKRLQDQENIFKALMTSTQKKSSLSAEELRNVVLI
                     GWVLGNTWLSYMESMGQKINETALQQAVEVMVQHYKPYLKEIL"
     gene            14653..15801
                     /locus_tag="PL000_12850"
     CDS             14653..15801
                     /locus_tag="PL000_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HNC89836.1"
                     /translation="MKKKTAIIIGAGIGGLCTTLALRKIGIEATVYERSGILGNVGAG
                     LVVAANAIRSLRRIGLGDEAVAAGSKLHLTQIRASNGDILASTDMSSYESLFGAPTIA
                     IHRAALHKVLMDALPASNIKLGAPCIRVEQDEEGVTAQFEDGSEARADFLIGADGVRS
                     IIRRQLFPSIELRYSGYTAWRGAVTSRDEVALGITSETWGRGNRFGIIRMDADRIYWF
                     ATANTKQGVTYAPDEQKKVLLDIFGSWHHPVKHLIESTPADVILHNDISDFAPMSQWS
                     VERITLLGDAAHATTPNMGQGACMAIESADVLAQCLSKNDDIVSAFKEYETRRKPRTA
                     FVTDRSWQIGRMAQWENPLACAARNFAVKTFSSHMMTKQLSKILGAES"
     gene            15798..16154
                     /locus_tag="PL000_12855"
     CDS             15798..16154
                     /locus_tag="PL000_12855"
                     /inference="COORDINATES: protein motif:HMM:NF025445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HNC89837.1"
                     /translation="MKNLIKFEELFMFAFSIFLFTKLDFAWWWYPVLLFTPDLSMIGY
                     IVNPRVGSWAYNLVHFKGLGLGMYFLGIMLANPILQLAGVILFGHSSMDRILGYGLKY
                     PDSFQHTHLGMIGKPN"
     gene            16255..17487
                     /locus_tag="PL000_12860"
     CDS             16255..17487
                     /locus_tag="PL000_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC89838.1"
                     /translation="MESKITVYGAYWCPDCRRSKKFLGENFIPFTWVDIEEDKEGEAF
                     VITKNGGKRIIPTIVFEDGSFLVEPSNAELAKKLNLKTEAKKTYYDLIIIGGGPAGLT
                     ASIYASREGADTLLIERSGLGGQAGITVGLDNFPGFPEGISGQEFAERVTQQAKRFGV
                     EILQAQDVEKIMADDGYNEVYTSDGKHYHARAVLITSGASYRRLDIPGEDDFIGAGIH
                     FCATCDGPFYKGAKNIVVIGGGNSACEEGLHLTTFAEKVTLLVRGDKLTASQVAIDKI
                     NEPTSRVEVKFNVAVDSFEGENSKLKKIHFHDSVTGEKSEISPEAAFVFIGQKPNTEF
                     AKEALELDRYGFVLTGHDFSHAQSGKIHGERLAFETSMSGVFAAGDARHGSTKQVASA
                     VGEGAASAISIREFLKRN"
     gene            17499..18473
                     /locus_tag="PL000_12865"
     CDS             17499..18473
                     /locus_tag="PL000_12865"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC89839.1"
                     /translation="MKYFITGATGFIGGYLARQLREAGHEVIAVVRNPAKAQTLSQLG
                     VTLHQGDVTEKESMRKPMSGVDGVFHVAGWYKVGVRDKSQAYAINVEGTRNVLELMKE
                     LNIPKGVYTSTLAVNSDTHGVEADENYHFTGKHISVYDQTKAEAHDIAVKMIKEGLPL
                     VIVQPGLVYGPEDTSSVRTSLIQYLTKKLPVMPKQTAFSWGYIEDIAHAHWLAMEKGK
                     VGENYNICGPTYKFTDALAIAKEITGVRLPMAVPAGMIRAMSAMMYVFDRFIPVPESY
                     TGEGLRIIAGVTYIGSNAKAKRELGYNPRPLKDGLEITLKHEMKLLGM"
     gene            18477..19001
                     /locus_tag="PL000_12870"
     CDS             18477..19001
                     /locus_tag="PL000_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89840.1"
                     /translation="MKTISLGRIFGLPIEILPLAFLGSFILWIGFTAIAFLGIKLPFV
                     ESLVLGLIATLLHWTFELIHGFGHAYAAKRTGYPMTGIALGTLAFFALTRYPMDEPEL
                     PPSIHIRRALGGPIINGLLSIVFFLALPLWPGDLFWIGIFALFENLFVYTLQVFVPLG
                     FNDGSTILRNLRKK"
     gene            complement(19089..20003)
                     /locus_tag="PL000_12875"
     CDS             complement(19089..20003)
                     /locus_tag="PL000_12875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNC89841.1"
                     /translation="MKNLKRILLGLLAVLAILILFLPLSIFVDSLFDGNRLDALVNTT
                     IPGVNGGPDVRAYVAKPEGEGPFPTVIMIHEFYGLRESIVGKADFLAKEGYLVIAPDT
                     FRGSTTSWIPRAIYQVINTKPEDVNTDLDSVYAWLETQSDVDAGRIAILGFCYGGRTS
                     LVYSLHNSKLAATVIFYGSPETDPEVLKSLPGPVLGIFGGADQSISVEEVDQFDKALT
                     IANIQHEITVYDGQPHAFVENVEGIQAGGAQGEAWAQMLAFLEKNLKNVTAQQNETSA
                     VSYRAPFAWKYYAMLVYEHAFGSASHQH"
     gene            20111..21007
                     /locus_tag="PL000_12880"
     CDS             20111..21007
                     /locus_tag="PL000_12880"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89842.1"
                     /translation="MKKIIITISTILFLAAVGLTAWNFDVVARSWRAAQEGRLSILKL
                     RYELAAFDIGRPSPNTPLNTKVSAVDGMMQVYVSEGIFVMGSNDDPRSKEFPAHGVYL
                     DAFWMDKYEVSNAMYAKCVDAGVCLPPVLRLNPYYGKWAYRNLPVVYANWYAAETYCA
                     WAGRRLPTEAEWEKAARGTDERRYTWGEDRPNPRLANFEETLMGEMLPVFRYPLGASP
                     YGVLNMAGNVREWVADWYAKDYYANSPKVNPTGPESGFERSLRGGAYDANADSITTIN
                     RFRHEPDSAGLSRGFRCAENAK"
     gene            21017..22915
                     /locus_tag="PL000_12885"
     CDS             21017..22915
                     /locus_tag="PL000_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89843.1"
                     /translation="MKKLINKPSPAVWQVICLLFSLGLYTLIMVNRSPNLLRPISMAL
                     RTGVGLVIPITTLVLYAVFRVKDRPGDLLSMAATLSLFAMPLAGLWASGLSQSVTISG
                     LLPLTDASFYYQDSLRIIMGQDISHFSAMRPFFPGFLSFLMYLTDRNFMFSLAIVTAI
                     AAIGIHYTVREIQRTHGTEVAVFLLVILFLYFRHHSGTSMSETLGVPLGALGMALIWR
                     SLEKRSQILAIFGLFICAFALNVRPGTMFILPLILLWSAWVLKKPSEFIAVKFLFIGA
                     AVIAVSFFLNSQYVRLLAEPSGTAFSNFSWALYGLASGGNAYTYVFEQHPELRLIADP
                     EQSRTIYRMALELIVQSPQLFVQGALHNWGMFFSDSWYSAFSFLESETGIVHRITRWI
                     IYTLCVLGFIKWLRKPSDPHSGLVAVAAIGVLASVPFVPPTDAYRVRLYAASIVTFGL
                     LPAMGISLLLNLFKLKLPSKADSEIQQSNVSAIFSALLVILVLVGPLVVKANSQPPPA
                     LEFSCPTGSDKIAIRFDAGSSINILREKDLFLDWMPNFHQGAFSRSIHDLADNDLIAY
                     FDTLRPGTSLISTVDTLSYKAALITIPTHLLPKPGNYLGLCGHWATEPRLQYYSLFTA
                     EEATLIER"
     gene            22967..23056
                     /locus_tag="PL000_12890"
     tRNA            22967..23056
                     /locus_tag="PL000_12890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23003..23005,aa:Ser,seq:gga)
BASE COUNT         5769 a         5966 c         5786 g         5467 t
ORIGIN      
        1 tcgccgtagg attgcagtgc agacatgaaa gccttgtgaa catggtctgc agggacggtg
       61 gtgccgtcaa attgcaggtc acgggtggcg caggcatctt cggcaacaat gaccttgaaa
      121 ccgaggtcgc ttgcagcacg agccgtggca tccacgcaca tatgactcat catcccagtg
      181 atgacaacac gttcgacgcc ccaacccttc aacaggtcaa gcagattcgt attgaggaat
      241 gaattgggct catgtttgta aacgatgggt tcgccttcaa agtgagccgc cgaatcgtgg
      301 atatcagaac cgctgtcacc tttgataaaa aaggttgcat caggctccag agaaatatgc
      361 tgaatgtgga cgtgatgtcc gccatgctcg cgaaagcact gcaaaagcat gtaggcgttc
      421 ttcgcagcag ccagcggatt tacgagctcg tattttccac cggggaaata atctttttga
      481 atatcaatga ccagtaatgc ggttttcata ttgctccttt gaaaaaaaca gcccaatggc
      541 gctgcactgc taaaatttgc tcacaaggca gccactcggc ttacacaaca ctcttcaaca
      601 attcttctgc ctgacgtttc aagtgtaact tatccgcttt ctctgaggca agccgaaaat
      661 cggcggcggc tttttcgggg tcattcaagg cttggtgaca ttttccacgc ccgaggtaga
      721 gatccgcttc gtgtggggcc aattgaatcg catgggtgta atcggcaatt gccttctcaa
      781 attccttgtt ggtgcggtac gcatcggcac gcccctgata cgccagaaat gagcgcggca
      841 attttgaaat ggcgcgttcg tagtactctt cagcccaatc gaggtaccct tcataaaggg
      901 tcatattgac cggtgaaacg gtcaacgcat ctttttctga taggcgcagg gcggcatcct
      961 gatctttatg tgcggcctca acattaccca gtttgaaatg cgccagtgag cgcaccacat
     1021 aaaaaagcgt aaattgcgac atcaacgaaa tggactggtt caactctgca agcgcaggcc
     1081 cgaactcctg tttatccatc agaatcgaag cgcggtcaaa atgcggcacg gcccagttcg
     1141 gactgagctc ctgtgcgcgc gcaaaatatt caagcgcaga gtcgtaatcc ttatccatcg
     1201 agtacaactc accacgcaat tccagggcaa tgtaattttt agggttgagt ttcagggcac
     1261 ggtcaatatc agccagcgcg cgttcttttt gcatcacgtt caagagacag gcggcacgtg
     1321 caagataatg agcaggctgt tcaggctccc gttctatctc acgggtatat aactgcgccg
     1381 cacgctcgta atctccctga aggtaataat aaccagaacg tttgaaaaga taattcaccc
     1441 ccaggttcac ccagcgcaac accgaacgat tgagcgttcc gatcgccagc gcgatcaaca
     1501 cacaggaagc cagaagaaga taatcacgtg tgtacagccc aaggacaacc aaacccagca
     1561 aaacgggaat actgaccagc atcgtgatca ggtcggcgct gcttcttcca aacaggccat
     1621 ccaacaccgc atgcatgata ttccccccat ccaacggata gatcggcaat aaattgaaaa
     1681 cgaccagaat gacgttcaaa agcgccaggg aaaaacacaa ctcaatgaag gtctgcaccc
     1741 agataaacaa ctcaggttca agcccacgcg gcgcaaagat atgcaaaagg atataccccg
     1801 ccacaaaaat gaatccaagc agcatgttca ccatgggccc cgctgcagca atgaccagat
     1861 tatgaaacgg cctttcaggt ttgcgttcga ggctggtcaa cccacccaac agccaaatga
     1921 tgatgctttt cacgtccaca ccaaccagtt tggccgccag cgcatgcccc aattcatgca
     1981 gcaaaataca aagaacaaac cccgtcaacc acagcagggc aagcactgta ctgcgcaagt
     2041 caatggggcg gaaaatgaaa taggtgatca acaggctgaa aatcatggaa aaatggaaac
     2101 ggacatccat cttgcctata cgcatcagcc gaattgagga tgacaaccac ttcaaaataa
     2161 atcactcgct tcgcaaccag accccaaacc tgctcgccaa aaattgaaaa ttacttgcgc
     2221 ccaaaatctg caggattctc accccacaac ccggtatccc acttgatgat cttatcttct
     2281 ggaagttcgt tctccgccag ccaattctcc gcactgcgga tgatggcaaa aaggacggcg
     2341 ttttttgggg tgtgtttcaa attcgttttg caaatccctg tggcaaccca ggcaatggca
     2401 ttttctgaat cggaataaat ggggacatgc atgttgtgtt tggcctgcca cgtcaacgca
     2461 tggacgatcg ccaggaactc ccccacattg ttcgtacctt cggcatatgg ccccgcataa
     2521 aaaatctctt cgcccgtttc cgtgttcaca ccgcgatatt cgagtttacc tgtcgagccg
     2581 ctgcaagccg catccacaca aatggacggc agaatcggtt ttgtgctggc cattttccat
     2641 ttgccatgtg tggaggattt ccccttgtaa tcttcgtact gttttagaaa agcagcatcc
     2701 gcctcggctt cgctttcaaa agccttgaac tgggcgccaa ccacgccctt cacctgcttc
     2761 tcacaatccg cccatgtggt gaagacaccc gtcttgcgtc ctttccaaac cacataaaac
     2821 tttttctttg ccatacatca attttacatc aaagtaaagc cgaagcgata aatacacccg
     2881 cccgtctcag tgaatatctg tcttgacaaa aagattgatt gacgtaaaat acgccaactc
     2941 ttatgaaaac caactattgc acatgtccat ggcggggatc gtaaccaccg cccaagacga
     3001 ccacccgcaa caagcagtca acgactttgg ccagtggaac gccccccgcg tcttttttga
     3061 cccacgcagg aaggttcgct gtccttttgt tttatgttga ctgcttgttt atttttcagg
     3121 ctattacaaa ctttagaaaa aggaaaccca aaacatgtct tacgctcatc ccgaatatct
     3181 ggtcgatacc gaatgggtcg ccaaccacct gaacgacgcg aacgtccgca ttattgaatc
     3241 ggatgaagac ccgttgttgt atgcaatcgg gcatattcca ggctcggtac aggtggattg
     3301 gttcagcacg cttcagcacc cgctgcgccg cgatttcgtc accaaagagc agttcgaaga
     3361 ggtggcatcg aaacttggca tcaccaacga caccacggtc gtcttttacg gcgacaagtc
     3421 caactggttc gcgtgctatg cgctgtggct ttttcaatac tacggccatg ccaatgtcaa
     3481 gatcatgaac ggcggtcgca tcaaatggga gcaggagaaa aggccgctcg tgaaggaagt
     3541 gccttcgtat gcccgaacaa cttacagcgc gaaagaagcg gataaatcca tccgtgcatt
     3601 ccgcgatgac atcttcaaac agatcgaagg aaagaggcca atggtggatg tccgttcgcc
     3661 aaaggaatac acgggcgagt tgacccacat gccgaattac ccgcaggaag gcgcgacccg
     3721 cggcggacac atccccggtg cggtgagcat tccctggtcg caggcggtca atgaagcgga
     3781 tgcgacgttc aaaacgaccg aagaactggt tgctctctac gaaggaaaaa acatcaaacc
     3841 cgacggagag atcattgcat attgccgcat cggtgaacgc tcgtcactga cctggttcgt
     3901 gttgaaatat ctgctgggat acaagaaggt caagaactat gatggttcat ggaccgaatg
     3961 gggcaatctt gtggatgccc ccattgaaaa atgagaaggt gttcgtcctt tggccgcctc
     4021 aggacgaatg agtgaggatt atgaccctgc caaccaaact gcaagagatc gtggatgata
     4081 tcgccagcat gagtcgcgag gaaaaactcg agaccttgat cgcgtatgcc gaatccatgc
     4141 ctgacctgcc tgaacgcttc aaggacgaac gctccaaaat ggagcctgtt cccgagtgca
     4201 tgacacccgt gttcatcatc gctgaaaagc tggaaagcgg cggcatcttt tttcacctcg
     4261 atattccccc gcaatccccc actgtgcgcg ggcttgcctc cattctcacc aacgggttga
     4321 atggctgcct gctcgaagag atcctctcgg tgcccgcaga tttttacatc ccaatgaatt
     4381 tgcaggaggc tgtgtcacaa cagcgcatca acggcttcat cggtgtgctg gcacacatga
     4441 agcaggcggc agtaaaggcg tcttcgtaaa atcgctcttg cgtacataac caataaaacc
     4501 aagatgctct cggcggcgtc cccgccgccg aggacggcgg cttgagaagg ctaataaaac
     4561 aacccgcagg gggtaccctg cgggttgttg attttcagtc tttcttttcc tcttcccacc
     4621 cttgttcttc aacagacata aaaatatcgg tggcagagat caggccgatc agcgacccgt
     4681 gttcatctgc aatgggcagg tgatggaatt tatgtttctg catcaattgc gaacactcca
     4741 tcaaggtcca ttcggcgcgg cctgtgatga tgggaccaga catgatctca cgtaccgtaa
     4801 tttcggcggg gttacggttc accgccacga tcttgtcgcg gatatcggtg gtggtgacga
     4861 ttccatagga tggattcttc tcgctgacat ccaccactac gctgtggatg ttgcggcggc
     4921 gcataagcgt catggcgtgc gaaacgcttg aatcggggtc aacaacaaca acagggctgg
     4981 acatccaatc tcgaactaaa tgcggcatgg gattctcctc aataagtagg tacagtttag
     5041 tttacttgat aaccaatttt acagcatcca tcgccagcca ggagccatcg gcggcgctga
     5101 tgtcttgaat ttccagatag acggttgttc ccgacggaat gccttcaagt gagaaggttt
     5161 cattgaacgt gccaggcgcg ccaaaatctg gcgcggtgac catcacaggg ccagcggcaa
     5221 gctgattgcc tgcttcgtca aagatgaaat aggagaggtt gttttcaaac ggcgcaatgc
     5281 tgaccgttcc gctgacctgc acactgcctg aggcttcggc tccgttggcg ggagcggcga
     5341 tctcaacgat tcgttttgcg ccttcggggg tggcggtggt gtagttgacc atccgcagtt
     5401 gattatttac aagggtgtag cggcgcgtgg ttggcaaggt ggggcaacag ccaccgtctt
     5461 gaaaaccgtg tacgatggca tcgagaacca cctcaccatt ttcaatggac atgctgttga
     5521 tcatgggacg gtcgtcaatg aaagtggaag tgaggaacac tggcagaccg ttcacgtttg
     5581 agtaaatggc aagcaggccg aaattacctg ttccgccgta gttttccaga aagatggcgg
     5641 cgatctcgtt gacaccatcc cccgtcagat cgccaacgga actgaactgg gtcaacccga
     5701 tgtaggcaaa atcgagggtt gtggcatcgg tgccttgctg atattttcca tcggtcaatt
     5761 ggatgacggc gtggtcgtca cgtgcgccaa gctggtactg ggcattcttg agttgatcca
     5821 cggtgaggct gggagcggac tctgtcggcg ctgggactgt ctcttgaaca ggagcaggcg
     5881 tcggcgcgtt ggaggcgggc gcagccaatc cacaggcgag ggttgcaaga gtcaatatca
     5941 aaacaagggc tagatggttg cggttcaaac agtctccttt ctatttgctg atggtgagtg
     6001 tggaaaacac ggtgtacatt ttgttcatta tggcatttcg gtcaaattcg gtgacggtat
     6061 cgggcgggaa gttgccaatg ttggcggaat gcatgtgata gatgacctcg taacagatgt
     6121 tgttgtaggc catgcggtag atctcctgct cgtacaggtt gccagcccca gccccagcag
     6181 atgtggagtg gacaaaggta aagccgttga tcacttcgtt tccgacgatt tgctcaattg
     6241 cgccgccgtt cggattcgaa tcggtgcagg tggcaaccat ttgcgggtct gcactggagc
     6301 cgaggaagaa gtaggcttca ctgaggtttg tttttacata agtttcctga tcgttcatgt
     6361 agagagccag ttcggggacg atcttgtaat tactgaatac aggaaaaaca ggcgcgaaat
     6421 aatcagaata cgtgagcgct acaccattgg cagggttgga atattggaag tattgctcgc
     6481 tcttaccggg gggattgatc gcaacccgca gcatgaattg aatgacatcg gtcgcagggc
     6541 tgagtgtgat gaaataatcc tgtgtggcag gcaatacccc gcgccaggcg gtgcaatcgg
     6601 actctgtttg cggacacaaa acgctcccat cggcgccttt gatctgcaag ggaatgtagg
     6661 tccagttatt gtcagcggat tgcggcagaa cagagaccga catgatctgc cctttcatgg
     6721 cgttgacagt ataagttttg ctggtattga tcgcgataaa gtcgttgata tcaacatacg
     6781 tgccgttggc agggaattgg atcgtattgg gacgggttga aggatcgggc gtgcttgtgg
     6841 gtgcgacaaa agttggaaag ccttcagtgg gcactggggt gaaggtcaac gtggggatgg
     6901 gggtaaattg aagcgcctga gtggggagcg gagtattttc cacaggcggc agtgtgggcg
     6961 gaatggcaag tgtgggggtt gagcaggcgg tgaggagcgt aagaagaaag aggaaagatg
     7021 ggattttttt catgggttgc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 ngcggtgagg agcgtaagaa gaaagaggaa agatgggatt tttttcatgg gttgcgaggg
     7201 tgaaggttta agtttgcagg gtcaacctaa atattcgatt acggtcgctt caccctgacc
     7261 gacaccgacg cacaatgtgg cgacgccgta tttcgcattg ccgcgattct tcatttcgtg
     7321 tacgagggtg gtgacaaggc gcgcacccga acagccaagc ggatgcccaa tggcaatggc
     7381 tcccccgttg acattgacca aatcgggatc aatgccgagg tcttcaatga ccgcgagaga
     7441 ttgcacggca aaggcctcgt tcaattccat caggctgatg tcttgaatcc gcaggccggc
     7501 acgttgaagg gctttctttg tggcaggaac agggccatag cccatcacac gcggcggaac
     7561 cccagccgaa gcggaggtga caatgcgtgc cagcggtttg agattcaagg ccttggcctt
     7621 ttcctcgctc atgatcagca gggcagccgc accatcattc aagcctgatg aattgcccgc
     7681 cgtgaccgta ccgccctcct ttttgaaagc gggtttgagt ttggcaaggg attccattgt
     7741 tgtatcacgg cgcggacgtt cgtctgtgtc tacaagtttt gggtcgcctt ttttctgtgg
     7801 aatggaaatg gggacgatct cctgtttgaa acgtcccgtg tcaatggcct taactgcaag
     7861 ctggtgcgaa cggaccgaga attcatcctg cttttcgcgg gtgaggtgcg ggcgttccgc
     7921 cgcaatattc tcggcggttt cacccattga gtcggtgccg tacatttcct tcattttcgg
     7981 gttcgggtaa cgccaaccca gggcagtgtc gtaagcggtg agatttccaa acgagaaacc
     8041 cgcctctgcc tttggaaggg aatatggcgc gcggctcatt gattcaactc cgcctgcaat
     8101 gtagatatcg ccctcccctg ctttgatggc acgagacgcc atgttgatgg cattcagacc
     8161 agaagcgcac aaacgattca cggtcactcc gcccacttca agcggcaggc ccgataacaa
     8221 cgccgccata cgcgcaacgt tacggttgtc ttcacctgct tgattggcac agccaagaaa
     8281 aacttcttcg atcaaggcag gatcaatttg gttgcgttca aggatcgctt tgatgacatg
     8341 cgctgccata tcgtcggggc ggacggaaga gagaattcct cccaaagcgc caatcggggt
     8401 gcggattgcg tcaatgatta caggtgtgtt catttgattt tctccatgtg agtttgcaaa
     8461 gcgattttac cacccgccat tgtaagtgag ctgtttcaca gaaaaggtaa tttccccttg
     8521 acaaatataa tcctgcttga taatattcct gtgtagttat ataaaacaaa aggaacactc
     8581 atgcccctcg aacacgccat cctcgctttt ttagaataca accccatgtc agggtacgac
     8641 ctgaaaaagc tcttcgatgt ttctgtcgcc catttttggt cggctactca gagccatatt
     8701 tacaaatcgc tggaagggtt ggaaaaaaag ggctgggccg aggcgcagat aatccctcag
     8761 gaagggaaac ccaacaggaa agagtatcaa atcacggcgg aggggcgaat cgaattgcgc
     8821 cgttggctgg tcacccccat tccactgggt ccagtccgtg aggcatgcct catccagatc
     8881 ttcttctcgc atttcagcac aaacgaagaa attgcggggc tctttgaaac acgcatgcaa
     8941 gagatccgcg agcatttgca tattttgaaa acggtggcac aaacggccat cgacgagaac
     9001 gccaaacaag tcggtgtgga acgcgcccgc caactctggc agatcaccct cgattatggg
     9061 attgattatt acgagttcga actggcatgg catgaaaaaa ccttgaagaa gattcacaac
     9121 ctgccgccgc tcactctgcc aaattcataa gtaaaacaga aataaaaagt aaaaaaactg
     9181 cctgaatgaa ggcaggtgtg ttttgtgcgc cttgcgcacg gaagggaaaa cagcatgttt
     9241 caagtattcg tcggtttcat cccctggatc atctactgga gtttttcagg accaggctta
     9301 tggaccattg caattttggg cggggcgacc gcggcgggcg gagtcgtggc atggcgctgg
     9361 cttaagcggc gcaacgtgaa aaccatggaa atcgtcacgc tgggatattt catcatccac
     9421 gccattctca cactgggatt cggatcttcc ttcctgaaga cctatggtcc catcgccaat
     9481 agtttggtgc tggcgttgat gtcggtcagc acactggcga tgaagaatcc attcacctat
     9541 caatacgcaa aagaagattg ggacaaatcc tactggaacg accccgcctt cattcggatc
     9601 aacgagatca tcacaagcgt atgggcggga attttcacgg tcaacacttt gctgggtgtg
     9661 ctggcagttg cgatccctgc acaaaaattg atctgggcgg tcatcgtccc aaacgtcggt
     9721 gttgtggtgg ggatcatctt ctcttcgcga ttccctgcat attattcccg caaaagcgtg
     9781 caggcacgtc tcgatgcatg ggacccgtac aaatggtcag cgccgaaatt tgacaagcgt
     9841 cctgctgcca caaatgaaca tgacgtcatc gttatcggct ctggcattgg cgggctttct
     9901 gccgccgccc tgctcgccaa acgcggactc aaagtagccg tcttcgaaca gcactacctg
     9961 cctggcggat tctgcaccgc atgggatcgc aacgtccgca ttcctggtca gcccatcgaa
    10021 tcgagagagc gctggacata catcttcgac gcaggtgttc atgatttcag cggactcggc
    10081 gagcgcggcg gaatccgttc gctgctgcga atgctcgata tcgagaatgc catcgagtgg
    10141 aaactcaacg agcaggaata ttttctgggc gagacccact tcaaggtccc gcatgatgcg
    10201 aacgaattta ttcagcaact gggcgagcgt tttccatccg aggcgcaaaa catccgcgca
    10261 ttcttcgatg aaatgctggg cgtttatcgc gatatgtatg ccgacatgga aaagctgggc
    10321 ggcgcaccgc gcacacccga taacgtggac gacctgttgg cgtatccgcg aactcacccg
    10381 cattcctaca aatggatgga ccgacctttc attgaaatgc tcgacacgtt tttcaaagat
    10441 gcccgcgtca aagaattgct ttgcgcgctc acaggctatc tcagcgacga ccccaagtcg
    10501 ctcagcgttg gcaacatggc gcccatcttc ggttatcact tcgatggcgg ctattatccc
    10561 gtcggtggtt cgcaggcatt tccaaacgca cttgtggatg cgatcgaaaa gcataacggc
    10621 aaggtgtatt tgcgcacacc cgtaaaacgc attctgaccg agaaaggtcg cgccgttggc
    10681 atcgaactca aagatggaac cacgcatcat gccaaggcgc tcatctccaa tgcagatacg
    10741 cacaaaacat tttttgaatt gctcgggcgc gagcacctgc ctgcagactt caccaactat
    10801 atcgactcgc tcaaaccatc caccactgcc ttcatcgtct ttttaggatt ggacatcacc
    10861 ccgaacctcg cccccatcac catgctggat gatatcggga tcatgatccc ctcgaatgtg
    10921 gatccgtctc ttgccccgcc gaaacatgct tctgtaactc tgttgaagct cctccccgaa
    10981 tcagagttcg ccaaatggga tcgcaaggct gaaggttaca accaacgcaa acgcgcatac
    11041 gccgaccaga tgatcgcctc cgcggcggaa gtcatccctg gcttgcagca gcacatcacc
    11101 tatcggcagg aaggcacgcc agctaccttt gcccgttacg catggacgac caacggctcg
    11161 atctacggtc ccgccatggg acaaaaacgc ctgaccgtga aaacgcccgt caagaatcta
    11221 tatgtcgctg gctcgggcgt gatgggcggc ggcgtggaag cggctgtcat cgcaggcatc
    11281 catgccgcga atgagatcta taaaaactaa cttgcaactt tcaacctgta accttcaact
    11341 tccaaactca aacgaggtgt caaaatgatt tacgcaattg ccaaatggat ccacattctc
    11401 tgcgccatca tctcgctcgg cgcgaacctg acctacttcc cgtggttcat gaccgtaccc
    11461 aaaacccgcg agacgctggt gtacaccctc aaaaccatcc gcctgctcga taactggatc
    11521 gccaaccctg cttatattct ggcatacgtc actggcgaga tcatgatgcg cgtcggcggg
    11581 cagatccact actccacgcc gtggatgacc gtggcgctca ttctgtatgt ggtcatctcc
    11641 atcctcggtc tttttgtcta tgctcccatg ctcaagaagc agcaacaact cgccgagacc
    11701 aaaggcgcgg acgatcccga ctatcaacgc gtttcgcgca atggaattat tttaggcacg
    11761 gtcattgtca tcatcaccat cggaatcact tatctgatgg caaccaaacc gcagttatgg
    11821 taaaccacaa agaggttata tgattttatt tctcaccctt cttcttgtca ttcttgctgg
    11881 tgcgggcatc tttgccctgc gccccgtcgt gcggaatatc cagaccgtat ttcacacaga
    11941 taaaactgtg gactatcaga tcaagtttca atctctgcag ttgatcctgg cagcggtcgt
    12001 gttggcattg acatatcttc tcaacccgaa aaacttccaa actttcttcc agcctggcaa
    12061 cgtcaatgca aacatcagca aaattccgtg gctcggtgtg acaggtcagg aaacctggtt
    12121 ggagatcgcc ctaaccctcg gttttttcat cactgttgca acgggcatct tcatgttcct
    12181 gcaattgaaa aaagcaggcg tggattatcg cttcctgctc ttcagcctgc tctggtcaat
    12241 tcctttttca gtggcgaatg ctttttctga agaagcgatc tttcggatgg ggattgtgtc
    12301 gccgctgttc ggcgtgttgt ccacatcggt cattcttttg ttatctggcg tgatctttgg
    12361 cgcaccgcat tactttggtc aacccagcgg tgtggtcggc gcagtgatgg caggcttcct
    12421 cggttggcta ttggcaatga gccttgtcga aacacagggg tttctcatcg catgggcgat
    12481 ccacttcgca caggacgtgg tcatcatcac ctccatgatt ctgatgggca ggaaacagtt
    12541 aagcaatcgt taaccgtttt ggcaattcag gcaggtagaa tccagaataa ggaggcagcc
    12601 atgtccaaga tcactcaaac caacggcaag atcgagatca ccgaaaccat gtcaacgggg
    12661 attcgggtgt tcctcttcct cctcggcttg cttccctggc tggctcccta tgaactgctc
    12721 atcaaaccag gctggacggg ctttcacata tcaactctgg tctttctcag catctccctt
    12781 ggcgccattg cagtcagcat tggctttatc ggcgcggcga tcttcggcat gaatcaaacc
    12841 gccaccttcg acctcacggc ccgcaccatc acccaccgct acgagaccac cgtcaacgcc
    12901 ttgcggacaa aacgctattc attcaatgac atcagcaaat ccgaagtccg tgaacataat
    12961 tgggatagcg ggcccaacac ctacagcctc gaaatccatt tcaacgacaa acacaagatc
    13021 ttgtgcggaa atttcccttc acgcaaagaa gccgaagaaa tattgaaata cttataggat
    13081 aaacatgtca agtatcgata aaaccatcct cgtcatcggc gggacaggta tgctcggcaa
    13141 acccgttgcc caacaactca aagcggatgg gttcaatgtc cgtctattgg ttcgtaaccc
    13201 agagaaagcc aaaaaaatat tcggggatgg gtacgagctc attcaaggcg atgtggataa
    13261 tgtcgcatcg ttaagcgccg cgctcactgg tgtggatgga gtccacatca gcctcaaggg
    13321 tggtcccacc gaagcggact ttgagcgcat ggatcacttc gctgtacgcg acatttccaa
    13381 gattgccaaa gaaaaaaacg tcgggcgggt cacgctcatt tcggcgtacg ctgtcagcga
    13441 ggaaaaggct gatacgccag agagcaggtc aaaggtcagg ggtgaggcgg ctcttaaatc
    13501 cagcggcgtg ccatatacca tcttccgcgc atcgtggttc atggagactt tgccactctt
    13561 tgtccaaggc aaaagtattt cgctgatcgg gaagcaaatc cacccgctgc attggatcgc
    13621 cgctgaagat tatgcgcgta tggtttcgaa gagttatcag actgaagaaa cgctcaacaa
    13681 agagctttac atctttgggc ccgaagcgta cacaatgggt gaggcaatga aaatttatgc
    13741 agaccatgca ggcgtgaagg tttcgccgat gtccactcaa atgctggcga tactgggtac
    13801 actcaccctc aacacggaat ggaagggcat gtcggtgctg atgaagcact acgagaattg
    13861 gggcgaggac ggctcgcccg atgaagccaa tcgtttactg ggtgctccgc aagtgacgtt
    13921 acgagagtgg ttagccagaa gttgagctcg ataaaacacc ttcgggtgtt tttttgccac
    13981 ttgacaattt agtaaatata tactaaatta gtaaacacat actaaattca ggaatcacca
    14041 tgtccccgcg aacctacaaa gcatcggaac aaaccaaaac caacatcctg caaaaagcag
    14101 tggaattgtt caatgaacat ggcacggctg gcatctcgat gaattcactt gctgaaacgc
    14161 ttggcatgag tgcgggcaat ttgcagtatc actaccgaaa caaggaagag cttttacgtg
    14221 cgcttctcga attgatgttc aaagaattcg atgcgatcta cccgtcaagc gcctcaactt
    14281 tcacactcga cacattgcgc aacctgatgc gcctcaactt tgagctttcg tggaaatacc
    14341 gcttcttctt tcgtgaactc ggcgccctac tccgcaatga tgagagtctt gcaatacgat
    14401 tccgtgagat tcaagaaaaa cgtttacaag accaggaaaa cattttcaaa gcgttaatga
    14461 ccagcaccca aaagaaaagt tcattaagtg cggaagaact ccgaaatgtg gtgcttatcg
    14521 gttgggtact gggaaacacc tggctctcat atatggaaag catgggtcag aaaattaacg
    14581 aaactgcctt acaacaagcc gtggaagtaa tggtgcagca ttacaagcca tatttaaagg
    14641 aaatcttata acgtgaaaaa gaaaacagcc atcatcattg gcgcaggcat tggcggactt
    14701 tgcaccacac tcgccttgag gaagatcggt atcgaagcca cagtttatga acgctcagga
    14761 atactcggta acgtgggcgc aggtttggtc gtcgcggcaa atgcgatccg ctctttgcgg
    14821 cgcatagggc tgggagatga agcagtagcg gcaggttcaa agttgcatct gacacagatc
    14881 cgtgcaagca acggagatat tctcgcatcc accgatatga gcagctacga gtcacttttc
    14941 ggcgcgccga ccatcgccat tcatcgtgcg gcattgcaca aggttttaat ggatgcactg
    15001 cctgcatcga atataaagct tggcgcaccc tgcatccgtg tggaacagga tgaagaagga
    15061 gtaaccgctc agttcgaaga tggaagcgaa gcgcgcgcag atttcctcat cggtgcagat
    15121 ggagtccgct caatcatccg ccgacaattg ttcccgtcca ttgagttgcg ctactctggg
    15181 tacactgcct ggcgcggagc cgtgaccagc cgcgatgaag ttgcgctggg catcacctct
    15241 gaaacatggg gacgcggcaa ccgctttgga atcatccgca tggatgcgga tcggatttat
    15301 tggtttgcca cggcaaatac aaaacaaggc gtcacctacg cgcccgatga acagaaaaaa
    15361 gtattgctcg atatttttgg aagttggcat catcccgtta aacacctaat cgagtccacg
    15421 cctgcagatg tgattttgca caacgatatt tctgatttcg cgccgatgtc tcagtggagc
    15481 gtggagcgta tcacgttgct cggcgacgca gcgcatgcca ccacgcccaa catggggcag
    15541 ggcgcatgca tggcaattga atcggcggat gtgctggcgc aatgtttatc aaaaaatgat
    15601 gatattgttt cggcgttcaa agaatatgaa acccgccgca aaccgcgcac cgcgttcgtc
    15661 acggatcgat cctggcagat cgggcgcatg gcacagtggg aaaatccgct ggcgtgtgca
    15721 gcgcggaatt tcgctgtcaa aactttttct tcacacatga tgaccaaaca attatcgaaa
    15781 atcctaggag cagaatcatg aaaaacctta ttaaatttga agagctcttt atgttcgcat
    15841 tcagcatctt cctgttcaca aagctggatt tcgcctggtg gtggtatccc gttttgttgt
    15901 tcaccccaga cctgagcatg atcggctaca ttgtcaatcc acgcgttggt tcgtgggcat
    15961 ataacctcgt acacttcaaa ggcttggggc ttggtatgta ctttttgggt atcatgcttg
    16021 caaacccaat actacaactt gctggcgtca ttctcttcgg tcattccagc atggatcgca
    16081 tccttgggta cggattaaaa tatcccgatt catttcaaca cactcatttg ggaatgatcg
    16141 gaaagcccaa ctaaaaaaca ttgtaataaa agaaactccc acctcatcaa ctcatgaggt
    16201 cacaacaagg ctgcgttata ccaaaaacaa aaatcaacag ccagaggaga aaacatggaa
    16261 tcaaagatca ctgtctacgg agcgtattgg tgtcccgatt gccgccgttc aaaaaagttt
    16321 ttgggcgaaa actttattcc tttcacctgg gtagacattg aagaggacaa ggaaggagaa
    16381 gcgtttgtga tcaccaaaaa tggcgggaag cgcatcattc cgaccattgt atttgaagac
    16441 ggttcctttc tggtagaacc atccaacgca gaactggcta aaaaacttaa cttaaaaaca
    16501 gaagcaaaaa agacctatta tgatttgatc atcatcggag gcggacctgc cggtttgacc
    16561 gcttccatct acgcctcaag agaaggcgcc gataccttgc tgatcgagcg ctcagggttg
    16621 ggtggacagg caggcatcac tgtggggctg gataatttcc caggcttccc cgaaggcatc
    16681 agcggacagg aattcgcaga gcgtgtcacc cagcaagcca aacgctttgg cgtggaaatt
    16741 ttacaagcgc aggatgtcga aaagatcatg gcagatgatg gctacaacga agtttacacc
    16801 tccgacggaa agcattatca cgcccgcgct gtgttgatca cctcaggtgc atcctatcgc
    16861 aggttggaca ttcccggtga agacgacttc atcggcgcag ggattcactt ttgtgccact
    16921 tgtgatggtc cattttacaa aggcgcaaaa aatattgttg tcattggcgg cggaaattcc
    16981 gcctgtgaag agggattgca tctcactaca tttgcagaaa aagtcactct tttggtgcgc
    17041 ggtgacaagc tcacagccag tcaggtggcc atcgacaaga taaacgagcc gacctcacga
    17101 gtggaagtaa aattcaatgt agctgtcgat tcgttcgaag gcgaaaactc caaactaaag
    17161 aaaatccatt ttcacgactc cgtcacaggt gagaaaagtg aaatcagccc ggaagcagca
    17221 tttgtattca tcgggcaaaa acccaacaca gaatttgcaa aagaggcttt ggagttagac
    17281 aggtatggat ttgttctaac gggccatgat ttttctcatg cccaatctgg aaagatacat
    17341 ggtgaacgcc ttgcttttga aaccagtatg tctggtgttt ttgcggcagg cgatgcgcgc
    17401 cacggcagca ccaaacaggt cgcttctgct gttggtgaag gtgccgcttc agccatttcg
    17461 attcgcgaat tcctgaaaag aaactagagg aaaataaaat gaaatatttc attaccggcg
    17521 ctacaggctt tatcggcgga tatctggctc gtcaattacg agaagcaggg catgaagtga
    17581 tcgctgttgt gcgaaaccct gccaaggcgc agactctttc ccagttgggc gtcaccctcc
    17641 atcagggaga tgtgaccgaa aaagaatcca tgcgtaaacc gatgagcggt gtcgatggcg
    17701 tcttccatgt ggcgggttgg tacaaggtcg gtgtgcgaga taaaagtcag gcgtatgcca
    17761 tcaatgtgga aggcacacgc aatgtattgg aattgatgaa ggaactcaat atccccaaag
    17821 gcgtttacac cagcacgctc gcggtcaatt ccgatacgca cggcgtggaa gctgatgaaa
    17881 attatcactt cacaggaaaa cacatcagcg tgtatgacca gaccaaggcc gaggcacatg
    17941 atatagccgt gaaaatgatc aaggaaggtc tgccgctggt cattgtgcaa ccagggcttg
    18001 tatatggccc ggaagacacc agcagcgtgc gtacttcgct gatccaatat ctgacgaaga
    18061 aattaccggt catgcccaaa caaaccgcgt tttcatgggg ctatatcgaa gacattgcac
    18121 acgcgcattg gcttgcgatg gagaaaggaa aggtcggcga gaattacaac atctgcggac
    18181 cgacatataa attcaccgat gcgcttgcga ttgccaaaga aattacaggg gtacgattgc
    18241 caatggctgt gccagcaggc atgatccgcg ccatgtcggc catgatgtat gtgttcgata
    18301 gattcattcc tgttcccgag agctacacag gcgaagggtt gcgcatcatt gcgggcgtca
    18361 catacattgg cagcaatgcc aaagccaagc gcgaacttgg gtacaatccg cgcccattga
    18421 aggacggact tgaaatcaca ttgaaacatg aaatgaagtt gcttgggatg taatttatga
    18481 aaacaatttc acttggcaga atattcggcc tgccgatcga aatcctcccg ctggcatttt
    18541 taggcagttt tattttgtgg atcggattca cagccattgc ttttttagga atcaaactcc
    18601 cgttcgttga atcgcttgtt ctgggattga tcgccacgct tctgcactgg acctttgaac
    18661 tgatccacgg atttggccat gcctacgccg caaaacggac cggctacccg atgacaggca
    18721 ttgccctcgg caccctcgcc ttttttgccc tgacgcgtta cccaatggat gagcctgaat
    18781 tgccaccctc aattcacatc cgccgcgcat tgggcgggcc gattatcaat ggattgttga
    18841 gcatcgtctt tttcctggca ctcccccttt ggcccggtga cctcttttgg atcgggatct
    18901 tcgccctgtt cgagaattta tttgtttaca cgctgcaggt ctttgttcca ctggggttca
    18961 atgacggcag taccattttg aggaatcttc gaaagaaata agaacgattt gctgaatccc
    19021 cattttcttc cagtctccag ccgccgagga cggcggctgg agcagtaatc aaatatcttt
    19081 ttacaattct agtgttggtg gctggcactg ccaaaagcgt gttcgtaaac caacatggcg
    19141 taatatttcc atgcaaacgg ggcgcgatag gaaaccgctg aggtttcatt ttgctgtgct
    19201 gtcacgtttt tcaagttctt ttccagaaag gccagcattt gtgcccaagc ctcgccttgc
    19261 gcaccgcccg cctgaatccc ttccacattt tctacaaacg catgaggctg accatcgtac
    19321 acggtgatct catgctggat attggcaatg gtcagggctt tgtcaaactg gtccacttct
    19381 tccacggaaa tggactggtc ggcgccgccg aaaattccca gcaccggacc aggcaaggat
    19441 ttcagaactt cggggtcggt ttcaggggag ccatagaaaa tgacagtggc cgcgagtttg
    19501 ctgttgtgca ggctgtacac aagtgatgtg cgcccgccgt aacaaaaacc aagaatggca
    19561 attcggccag catctacatc tgattgggtt tcgagccacg catagactga gtcaaggtct
    19621 gtgtttacat cttcaggttt ggtattgatg acctgataga tggcacgcgg aatccatgag
    19681 gtcgttgagc ctcggaaggt atctggcgca atgacaaggt agccttcttt tgcaagaaaa
    19741 tccgctttgc caacaatgga ttcgcgcaaa ccgtagaatt catggatcat aatgacagtg
    19801 ggaaatggcc cctccccttc tggcttcgct acgtaggcac gtacatcggg tccgccattc
    19861 acccctggga tcgtggtgtt gacaagggca tcgagacgat tcccatcgaa cagggaatcg
    19921 acaaaaatgc taaggggcag aaacagaatc agaatcgcca aaacagccaa cagtccaagc
    19981 aggatgcgtt ttaagttctt cattgaaata ctcctaaaag atttaatatt cagattttat
    20041 tctggaaatc aatcaaatcc tctcaacaaa tgccgcttta caagtagagt aaaatcaatc
    20101 cgtcatgtca atgaaaaaga taattatcac catctctact attttgtttt tggccgccgt
    20161 gggtctgacg gcctggaatt ttgatgttgt tgcacgttcg tggcgggccg cacaggaagg
    20221 gcggttgagc atattgaagc tccgctatga gctggccgcg tttgatatcg gcagacccag
    20281 ccccaacacc ccgctgaata cgaaagtatc cgcagtggat ggcatgatgc aggtctatgt
    20341 cagtgaagga atctttgtca tgggcagcaa cgacgatcca aggtcaaagg aatttcctgc
    20401 gcacggtgtg tacctcgatg ctttttggat ggacaaatac gaagtaagca atgcaatgta
    20461 cgcgaaatgt gtggatgctg gtgtatgcct gccgcccgtg ttgaggctca atccatatta
    20521 tggcaagtgg gcgtatcgca atctgcctgt ggtgtatgcc aactggtacg ccgctgaaac
    20581 gtattgtgcc tgggcaggca ggcggctgcc cacagaagcg gagtgggaaa aagccgcgcg
    20641 cggcactgat gaacgccgct acacctgggg tgaagaccgt cccaatccgc gcctcgcaaa
    20701 ttttgaagaa accctgatgg gcgagatgct ccctgtgttt cgctatccat tgggagccag
    20761 cccttatggg gtgttgaaca tggcggggaa tgtccgcgaa tgggtggcag attggtacgc
    20821 caaagactat tatgccaact cgccaaaggt caaccccacc gggcctgaaa gcggcttcga
    20881 acgttccctg cgtggcggtg cctacgatgc caatgcagat agcatcacca ccattaatcg
    20941 cttccgtcat gagcccgact ctgctggatt gagccgcggc tttcgatgcg ccgaaaacgc
    21001 aaaataaaat cattcgatga aaaaacttat caataagcca tcccctgccg tttggcaggt
    21061 catttgcctt cttttttcgc tcggcttgta cacactgatc atggtcaacc gttcacccaa
    21121 cctgctgcga ccgatcagca tggcattacg cactggtgtc gggctggtga ttcccatcac
    21181 cactttggtc ttgtatgctg tctttcgtgt caaagaccgc cccggcgatc tactcagcat
    21241 ggcagccaca ctctccctgt ttgcaatgcc gctggctggg ctgtgggctt cgggcctgtc
    21301 acaatctgtc accattagcg gattgcttcc attgaccgat gcgtcctttt attatcaaga
    21361 cagcctgcgc atcatcatgg gacaggatat ctctcacttc tccgccatgc gcccattctt
    21421 ccctggattt ctatcctttt tgatgtacct gacagatcga aatttcatgt tcagcctggc
    21481 catcgtcaca gccattgctg caattggaat tcactacacg gtccgcgaga tccagcgcac
    21541 acatggcact gaagtggcgg tattcctgct ggtgatcctt tttctttatt tccgccatca
    21601 ttcaggtaca agcatgtctg aaacgctcgg cgttccgctc ggtgcccttg gcatggcact
    21661 gatctggcgc agtctggaaa aacgctccca aatcctggcc atcttcggct tgttcatctg
    21721 cgcctttgcc ttgaacgttc gtcccggcac aatgttcatt cttccgctca tattgctttg
    21781 gtcggcctgg gttctcaaaa agcccagtga attcattgcc gtcaaattcc ttttcattgg
    21841 ggcggctgtc attgcggtga gtttcttttt aaattcccaa tacgtgcgcc tgctggcaga
    21901 accatcggga accgcctttt ccaatttttc ctgggcattg tatgggctgg cctcaggagg
    21961 caatgcctac acctacgttt tcgagcagca tcccgaactg cgtttgatcg cagaccccga
    22021 gcaatcccgc accatctacc gcatggcatt ggaactgatc gtgcaaagtc cgcaattgtt
    22081 cgtgcagggc gcactacaca actggggtat gttcttctct gatagctggt atagtgcgtt
    22141 ctcttttctt gaaagcgaaa caggcattgt gcatcgcatc acccgctgga tcatttacac
    22201 cctgtgtgtg ttgggattta tcaaatggct tcgcaaaccc tccgacccac acagcggact
    22261 tgtggctgtg gctgcgattg gcgtactggc atcagttcca tttgtcccac ccacagatgc
    22321 ctaccgcgta cgactgtacg ccgcctccat cgtgaccttt ggcctgctcc cagcaatggg
    22381 catttccctc ctcctcaacc tgttcaaact aaaattgccc tccaaagcgg attctgaaat
    22441 tcagcagtca aatgtttccg ccatcttcag cgccctgctc gtcatccttg tgctggtcgg
    22501 gcctttggtg gtcaaagcca attctcaacc accacccgca ctggaattct cctgccccac
    22561 aggcagtgac aagatcgcaa ttcgtttcga tgcaggtagt tccattaaca tcctgcgcga
    22621 gaaagatctg ttcctcgatt ggatgcccaa ttttcatcag ggtgctttca gccgcagcat
    22681 ccacgacctg gcagataatg acctgatcgc atatttcgac acactccgcc ctggcacatc
    22741 attgatttcc acggttgata ccctttccta taaagcagca ctaatcacaa ttcccaccca
    22801 cctcttgccc aagcccggaa actacctcgg gctctgtggt cactgggcca cggaaccacg
    22861 cctgcaatac tacagcctgt tcaccgctga agaagccacc ctgatcgaaa gataattcaa
    22921 gtattgccat acaaataaca catggtataa tctgcccgct gaatttggag aggtcgcgta
    22981 gtctggctta gcgcgcacgc ttggaaagcg tgtaacccac aaaggttcgc gggttcgaat
    23041 cccgccctct ccgccagaaa aaccgctttg ctttgcaagg cggttccc
//
LOCUS       DAOQLC010000069        22912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_766_length_22912_cov_26.215820, whole genome shotgun sequence.
ACCESSION   DAOQLC010000069 DAOQLC010000000
VERSION     DAOQLC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_22912_cov_26.215820"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..1275)
                     /locus_tag="PL000_12895"
     CDS             complement(304..1275)
                     /locus_tag="PL000_12895"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89844.1"
                     /translation="MNSALKILILATLFTLGACASPTEPPVPTETPIPPSPTADLGIG
                     PFLTVNKAGDLYLKSPYGAAVNQAGEIYISDAGNSRVLKFDSSGTLLAAWDKKGSGDG
                     EFNSMGFGGLAVDPSGNVFVVDNGNHRIQKFDGDGNFITAWGTEGTGDGQFVRAIGIA
                     VDKDGNVYITDDGNPFVQKFDNDGNFLMKWGGAGSTTGKFSHATGIAVDGNGNIFVAD
                     YENKNVQKFSADGSFITTWDIRNADGKKGTPEGIVVDVQGQVYISDYDLGQIQAFTND
                     GELLWSWTDQDRNDGYFSRPTGLALDGKGMIVIVNQSGGGWEIFKLP"
     gene            1342..2508
                     /locus_tag="PL000_12900"
     CDS             1342..2508
                     /locus_tag="PL000_12900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC89845.1"
                     /translation="MKILTVLTYYRPHTSGLTIYAERLARAFVKRGHEVTVMTTHYDP
                     SLPLEEMMDGVKVIRIPVIARVSKGVIAPTFGLVATKLVWQHDVVQLHLPQFDAPGVA
                     MRARLFGKPVVLTYHCDVQLPQGWFNRFVNSVLDFQNDMAGRLANHIVTYTQDYADHS
                     PYLSRYASKLTPILPPVEMPVPAPEAVTAFAEKHRTKELKPVIGMVTRFASEKGVEIL
                     LDALPIILKKYPNAQVLYAGQHQNVMGEQAYFDRLIPKIHEYETSGHWTFLGNLTPVQ
                     LAALYPNLDVLTVPSLNSTEAFGLVQIEAMMNGVPSVPSALPGVRRPVQMHGMGVVSE
                     IGNAQSLAASILEVLDTPAKYRGDVDGIKTTYDPDSVAQEYEKMFERLKGRTTG"
     gene            complement(2498..2893)
                     /locus_tag="PL000_12905"
     CDS             complement(2498..2893)
                     /locus_tag="PL000_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC89846.1"
                     /translation="MIYVDTSAFLAICNPEDPSYQIALDTWKNLIEKGERLASNNYVL
                     VESIALIQNRVGLEALSILHNDIIPFLEIEWLDESLHNGVMDQVITGNRRQISMVDYS
                     SFATMKRHNIRTAFTFDDHFRQHGFEIIP"
     gene            complement(2890..3147)
                     /locus_tag="PL000_12910"
     CDS             complement(2890..3147)
                     /locus_tag="PL000_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HNC89847.1"
                     /translation="MVQLTEEQVKTLKKMARVRKASVASLVRESVTLYVDASKVNPQM
                     DEKRRRALEGLEKIKKAHFKDIEGKTDVAKNHDKYLNETYS"
     gene            3206..3967
                     /locus_tag="PL000_12915"
     CDS             3206..3967
                     /locus_tag="PL000_12915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89848.1"
                     /translation="MSQKDFLFLQVSSLPYFRAFLRAVESSYYQDLPLPAPVYDVGCG
                     DGHFASLTFDQKLDVGLDPWRFSMREAKKYNAYKSLVEADGSRAPFPSNHFESGLSNS
                     VLEHIPHIDAVLAETARVLKPGAPFYFCVPNTRYFSALSLTKVFGKPYENWFRKISRV
                     HHADEPDVWEKRLEAAGFKLERWWHYFSPASMRVLEWGHYFGLPSLVAKALTGQWILS
                     PTKWNLSLTENYLRKYASPEPVEDGTFTFYIARKK"
     assembly_gap    4008..4107
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4108..4853
                     /locus_tag="PL000_12920"
     CDS             <4108..4853
                     /locus_tag="PL000_12920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89849.1"
                     /translation="ITNYQLPITPMSFDEKYFSTHTYANITFAKYSMYWWSNRFYAML
                     ARRYGRRGARLLEVGSGMGHLVGQLSAWFEAYGMDLNHWAVNKSKADAEAASLQTASA
                     QDLPYQNGAFNVIIIKHIVEHLPDPAKAIREIARVTEKGGILILATPNLGSLLKPWKG
                     KTWIGYQDPTHISLKQPEEWLEIIRGAGFETLRIFSDGFWDVPYIRYVPKQIQKLLFG
                     SLGGLQAITGFVFLPMRWGESIIVIARKR"
     gene            4875..7067
                     /locus_tag="PL000_12925"
     CDS             4875..7067
                     /locus_tag="PL000_12925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89850.1"
                     /translation="MNEPSVLDFVKAIFKNWNSFQSFLQALFKRGDASQLMQSADEAT
                     IQLDPQPDPEPVEAPAPKKLPWRTLLVLFLALAAQQTFEPPRTSTANLGVALYIAAFG
                     LAVWAYLRGEWTMTPVSQIVPGVEDASIRRGSFVLSVIFSAATFWMMGGNKFTALNLT
                     LWVLAIVFFLRAFWLNEPRSAASSNFDLKSIFTSSEWNIRITRSALAVFVMAVIIIFF
                     RTYRLYDVPPEMTSDHAEKLMDVYDITQGQFSIYFPRNTGREPLYIYLCALFAELFTG
                     TSFLTLKVVAVIGGLLTLPYIYLLGKEFGSERIGLLAVLFAGIGYWPSVIERFGLRIS
                     FYPLFAAPTLYYIIRGLRRQNRNDFILAGIALGIGLNGYTPFRIMPFVVVALFGVYLL
                     HARDWQARKQSIIWLALLAFTSFVFFIPLARYMIEFPDMFVYRAFSRLSTVERPLPAP
                     IWQLIPLNLWNAAKEFNWYNGDIWVHSIPGRPALDIVSGALFLLGVSLMLFRYIRSRS
                     WMDLMLLLAVPFFQMPSILSLAFPEENPSLNRTGAAIVPVFLLVAIGLDTLLNSINLR
                     EKANEESIMSENRRAMGHRPLLAGTIFVLLFLFSFTQNYNLVFHTYYDQYRASAWNSS
                     EMGAVMKGFMERGGSADNVWIVPFPFWVDTRLPPFWAGVPGRDIAIAPDNIPATVELS
                     GDKLFMFTLDDTVTMSELKRLYPQGTLSQFHSAVPAHDFYVYQIPASQ"
     gene            7115..9154
                     /locus_tag="PL000_12930"
     CDS             7115..9154
                     /locus_tag="PL000_12930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89851.1"
                     /translation="MQNEPSHLKAFGFPWRSLAALVLALTAQLMLEPPAKIWVGIVLY
                     IAAIGLVVWSFLRGEWVIAWIAEDIYHGDTEALGFKRFFSVALWLRGSKQILFFLSLP
                     LLGAAFYFFRGNQFTVLNISLWLTGLVLFIVAFWSTTARTADFSRLGVNRLKSAVQKM
                     DWEWLVLLLAVFVFAAFFRFYRLNEVLAEPFSDHAEKLLDVYDISKGNTSIFFIRNTG
                     REGLQMYWTFLVAKIFGTGISFFSLKLGTALIGLFTLPFVYLLGKEFGNPVVGFFAMF
                     LFGISYWMNVTARIGLRFPLYPLFAAGTLLFLTRGLRTASRNDFLLCGLLLGLGLHGY
                     TPFRIMPFVVLTAFAIFILHNNSKAIRVQAVQGLTIIAVTALMVFLPLLRYWLEYPEV
                     FGLRAFSRLDSAGTFGSLMGVFFSNLANALLMFNVDDGNIWVNSLPHRPALDVVTGAF
                     FVIGLVLLGLRYLRQRDWRDLFLLVLIPLLLMPSVLSLAYPEENPALNRAGGAAVAAT
                     LIAALAVEGLVVGTVRGVESGQGPEKRRGFILHILVGVLLAAASFQNYDLVLRQFDRY
                     FRIGAWNSSEMGRSIKFFGDVYGRTDTAWIVPYEQWVDTRLPALWMGDPNRDFALWPS
                     QFADTLAMPAPKMFIFNLRDLDTKNALKQLYPNGTLSRYTSATTGKDYMIFFVEE"
     gene            9211..14112
                     /locus_tag="PL000_12935"
     CDS             9211..14112
                     /locus_tag="PL000_12935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNC89852.1"
                     /translation="MTEKRAWFYDLLFVCILLIAGFLRLTGVEWGEGYHQHPDELFLV
                     GVLDNLRAHTCADAVTPVDLCKPEDQRWMTLGEYFDTATSTLSPYNRGHAFFVYGNLP
                     MTVTRIAMEMTGNDDIGKSKFFARQFSALADLFTIFILYLIVSNLYGRRIGLFAAVFS
                     TFAVMQIQQSHFFTTDLFVNLFMFLAIMFAMGIVEKRDLGIGNNESETLQLTSSDAGF
                     SNYLLRLVRSPLFLLSIGFGFALGMAMASKINAAALAILLPGAFAVRFLVQDKDKKLA
                     ADYWSLITVFLIAGGLATIISFRIFQPYAFNGLGLNDQWLSNIAEQRVQANGDADLPW
                     NLQWARRTHLYSFTNLTVWGLGLPLGILAWVGFLLMGWRILKGEWRHLLLWGWTAFYF
                     LWQSLQFNATMRYQLPIYPLLCMMAAWALFELPKLRGETGRFKLFEVLVATMGVVVLV
                     TTTIWAFAFQSIYTRDEPRMAASRWLFQNAPGPVNVKIKMADNTIYNQPLPVPMDMAI
                     QAGAPFTMTFSANRDGDISEIVFGHAIDLESPSTIVLTLASASAPDLVLARASEKVDY
                     SQTKDPRGPAVEFKLDETVPLVKGQAYLLKIETLGHALTLSGSAIANETGYDWGLPFR
                     IDAYDAFGGIYRGDLTLEVYWDDNPDKVNRYVSMLSMADYIVIPTNHQYAQITRLPER
                     YPLTTYYYRKLLGCPENADIIECYRNAKPGQYEGGLGFELVKVFETYPQFGPIIINDQ
                     AAEEAFTFYDHPKVLIFKKTSDFNADKLSAMLNTVDLTNVVHLTPKGADNYKSLMLSK
                     EQYAAQRAGGTWSQLFNYEWLQNKYPVIGLLLWYAFIFLLGLAAYPIVRLALPGFRQY
                     SYPLGRMAGLSLLAWISWMGGSLGVPYTRGTIGAAFVLVTVAGLGLWWIRKAQFKDEW
                     DSNRKFFTLVEIVFAVFFLIDLLIRFGNSDLWHPSKGGERPMDFSYFNAILKGTSFPP
                     YDPWFAGGYINYYYYGFVLAGTPVKLLGIVPSIAYNFILPTWFALVAVGAFAIGWNLL
                     EKEEGASSNFSAQHSSLLSGLSAAVLSVLLGNLGTIQMIFQALQRMAAPGGIITDATF
                     VQRWIWAFQGFMMSFGPGASLPIGRGEWYWNPSRVIPPGPGNEITEFPLFTFLYSDLH
                     AHMLVMPLALFVIAWALSFIKSRANLRREEWAASFLLGAMVLGAVYPTNLSDFYTYLL
                     LALVVLAFVFLFRAEVSGLRLLEDLPDAVKRIALMLVSIGALTVLSFVMYQPYRSAYS
                     QSYGALDPWTASHTPVSSYLTHWGLFLFIIFAWMAWETREWMARTPLSSLASLRPFAI
                     LIELALAGIFVVLVYFAYEGIWVGWLALPLAAWAGVLLLRPDQPDEKRLVLFLIGTAL
                     MITLVVELRTVRGDIGRMNTVFKFYMQAWLMLSVSAAAALGWMFSDAPRWSAGWRNTV
                     QVILLTLVFGATLFTFTATSDKVTDRASTDTPRSLDGLNYMNYSTYFDHGQNLTLNED
                     YRAIRWMQDNVQGSPVIVEAAPAGRQYEWHSRFTIYTGLPGVVGWQWHQQQQRVLFSQ
                     QIIDRGIEVDAFYNSLDQDSVRKFLAKYEARYIIVGQLEQAKYMPIDPGIPNGLMKFD
                     LWNGTLWNEVYRDGDTVIYEVIQ"
     gene            14109..14465
                     /locus_tag="PL000_12940"
     CDS             14109..14465
                     /locus_tag="PL000_12940"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HNC89853.1"
                     /translation="MILHITSRAEWSAAQARGEYIAPSLQTEGFIHCSTDKQVAHVAN
                     AFYRGRNDLVLLMIDEAKLKPELKWEPPAGPPAPGISESDSFPHIFGPINLTAVASVL
                     DFEPDPASGTFSYPKA"
     gene            14462..15079
                     /locus_tag="PL000_12945"
     CDS             14462..15079
                     /locus_tag="PL000_12945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HNC89854.1"
                     /translation="MTEPTAALRIRPAHAADSALAAKVIYMSMGGLADYLFDNNGQAI
                     QTILAELFTRDAGRFGFRIAMVAEDETQPVGMLFACPGARLAGFNMASFQHFFPVMGF
                     KHALKFMWRGVTLPGGPEAEKDEYYVSNLGILPEAQGRGIGTSLLAYAEETCRAHQLS
                     KCSLIVGMHNENAFRLYQRLGYQVVETAHAPNEHLAYYRMVKHLN"
     gene            15111..17627
                     /locus_tag="PL000_12950"
     CDS             15111..17627
                     /locus_tag="PL000_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HNC89855.1"
                     /translation="MTAFIAWYFILTLLGWLTFPLAHFLFPALKDRGYTLSRAAGLLI
                     WGYSFWLMASLGIAQNDLGGILLALIVLASLSAWVLANQRSEIIDFFRTHRSLVITTE
                     VLFLVAFAFLAFVRSANPELTGTERPMELAFINAILRSPVFPPQDPWLSGYAISYYYF
                     GYVMTAMLAKVSGIYGSMAHNLMTALVFALGAIGSYGILYNLLSHGHEPKKTEDGDMD
                     TAGSHRSSFSIYGFLSLLAPLFLLLISNLGALLEVMHQWGLFWKQDYISGEYTSAFWK
                     WMDMKELSQSPILPFHAPPLDRYLWWWRSSRVLQDYDMLNRPLEVIDEFPFFSFLLGD
                     LHPHVLAIPFGLLAIAAALNLFLGGWRGEVKLPLGARLQISGPGFLFTALLLGGLAFL
                     NTWDILIAAALIVASYVLWCANEEGWRWDRLEDALLFGFPLGILAILMYLPFYVGFSS
                     QAGGILPNFMYPTRGIHLWVMWGTLLVPILAYVLYLWRGEKFPANWKLGVSLGLGFVV
                     FLLVFSWLLGVIGSILEKDFVNGFLASQGMTASQFFGATLMRRLSYIGGLITMLAVFI
                     PSLAFLFSQKQQGQPEAELSAPVENNRKPETQFVLLILILGAILIITPDFVYLRDKFG
                     YRINTVFKFYYQAWMLWSLAAAFAASRLLQSLRGAANVAFRVALVLVVSTGLLYPVLG
                     LITKTNNFNPPYGFALDDFERVIRETPEEAAAINFLRTAPDGVVAEAIGDSYNYPFTR
                     ISTHTGLQTVLGWPGHEDQWRGSYIPQGSRRDDINRLYTTVRWEEALQIIEQYNIRYI
                     FIGTLERTSMNVSEEKFIAHLKPIFQQGGTVIYEVPVLEK"
     gene            complement(17673..19364)
                     /locus_tag="PL000_12955"
     CDS             complement(17673..19364)
                     /locus_tag="PL000_12955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC89856.1"
                     /translation="MTTDFSSLNLREEITQAITELGYSEPTPIQAGMIPLMLTGVDVI
                     GQAQTGTGKTAAFALPILNNFQHQKNPQALVLAPTRELALQVADSMTEYGKHLNLRVL
                     AVYGGQPYSPQINSLKRGVDIVVGTPGRLNDLLERGVLILNEIKTVVLDEADEMLNMG
                     FIEEVEKILSMTPSERQTALFSATMPPRIRALANRFMRDPQSVVIKRSTLTAAAIEQR
                     YYLVHDNDKINALTRLFEVEPIHSALIFARTRAETGQLANELVIRGIPAEAIHGDLDQ
                     NARERVLGRFRANQLKVLVATDVAARGLDIDDISHVFNFHLPDDAEVYVHRIGRTGRA
                     GKTGIAISLISPREKRRMREVEALTKQLVTKMELPTASEIMRHRESLVVENLKIWLGR
                     ARYKRELEMVQELIEAGHDVMNIAAAAIKIARADEKQRPIAEVSDVKTDFTRKPEREF
                     GRRDSKGGKREFGAGSAAKGSKREFGDKKSRRDSHEEGMVRLKINKGKAHNIRPNDIV
                     GQIAFHANIPGYTIGKIRIEEKVSFVDVPADAVDQVLKHSGNYKIGKEKFSVAKP"
     gene            19651..20055
                     /locus_tag="PL000_12960"
     CDS             19651..20055
                     /locus_tag="PL000_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC89857.1"
                     /translation="MAAKNVHLNWNPDQQFTLQDNDGYQLVMKKPKGVSASDLLPMAL
                     IGCASHDVVEILNKQKQDLRQLNVTAESIQDETPPWRFRKIHIHFQVVGKGIDPEKVH
                     KAIQLSEEKYCSVYATLKDAVEIINDFEVVEA"
     gene            complement(20145..20945)
                     /locus_tag="PL000_12965"
     CDS             complement(20145..20945)
                     /locus_tag="PL000_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89858.1"
                     /translation="MKLKQRFPFLVLITLSAALMIVLPLIRFFTLQPQQDALQQLEPS
                     FQFRVFFLGLTDALWKAVLILLCFWSGVGINRLLLRIQDSAKRKLAINGVIFGSLFLC
                     VVLPILGLILTYTFFSPKESWVQLPDPPESARTISGGIYNRIIIETEKGNYFSHTIPD
                     DGLGWLADEKPDASLLQEMDESTSPNIEAPGVSTNLIGVPAYPGSTQKIYYTILEDHT
                     VWYLPQTGNTFFTTVLLAAVSIPILIGGLLMLLGMGTISILDKLAERI"
     gene            complement(21106..21435)
                     /locus_tag="PL000_12970"
     CDS             complement(21106..21435)
                     /locus_tag="PL000_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89859.1"
                     /translation="MKKANKTSQTEDMLPEYNLEGKKGVRGKYVKALQAGYSVRVMHE
                     DGTVTMRHFVPKESAILLDKDVKAHFPDSKSVNAALRSLIRKRAKVVVEKQKKVRSIA
                     AKARRSK"
     gene            complement(21419..21715)
                     /locus_tag="PL000_12975"
     CDS             complement(21419..21715)
                     /locus_tag="PL000_12975"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNC89860.1"
                     /translation="MKIIFEWDENKARTNFEKHKVSFDEARTIFVDPLLVTFADELHS
                     EKEERFISIGLSENNRILLVVHTEREEKKETIIIRIISCRKATPTERKRYEKGE"
     gene            22146..>22912
                     /locus_tag="PL000_12980"
     CDS             22146..>22912
                     /locus_tag="PL000_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89861.1"
                     /translation="MTVAICIYWLFSGTFDISNDLGGVAGIIAVPLLFTIAAFRSYIL
                     QNMPSATEAVEQDPRPPVLYLRSFDQEMLKFVHLKDEEKARYNTFLNYAGDVNLPPFE
                     QAIYTALNSSALLTFGSVESEDVTFEKYFRGEVMERIGPFVALGNPIDSLPTEGAFRD
                     YQIDERWKDVFFNHSERAACILMQLGSSDNLQYELTAILSRRISHKLFILTPPQENLK
                     DRYAWVRKYILREKPANWQAFSAILQKNGFRIPDEPLG"
BASE COUNT         4797 a         5919 c         5890 g         6206 t
ORIGIN      
        1 tattttgagc tgattcgctg gagcagcgtg attcacccca aacgtaagga atcagccgcg
       61 aatattttca aaagtctgaa agattaacca cggagaagaa aacaattaaa aaccccgtcc
      121 gcaggaggct ccgcactgcc tcagtgtctc cgtggttcaa atgaattgca agatacggac
      181 gagttataaa aagccgaccc tgtaaaatgg atcggctttt ttcgtgcaaa ttcgcggcta
      241 aagaattgaa agattaattt cgggatattt catgtttgtt ttcatgcttg gatgtaaaaa
      301 cggctacggc agtttgaaaa tctcccaacc gccaccggat tgattgacaa tgacaatcat
      361 gccttttccg tccagcgcga ggccggtggg acggctgaaa tacccgtcat ttcggtcttg
      421 atcggtccac gaccagagca attcgccgtc gttggtgaat gcctgaattt gaccaaggtc
      481 ataatcgctg atgtagacct gcccctgaac atccactacg atgccttcag gggttccctt
      541 ttttccatct gcattgcgga tatcccatgt tgtaatgaaa cttccatcgg cgctgaattt
      601 ctgcacgttc ttattttcgt aatcggcgac gaagatattt ccattcccgt ccacggctat
      661 gccggtggca tggctgaatt tgcctgttgt ggagccagct ccgccccatt tcatcaggaa
      721 gtttccgtcg ttatcgaatt tctgcacaaa gggatttccg tcgtcggtga tgtacacatt
      781 cccatcttta tccacggcga tgccaatggc gcggacaaat tgaccgtcgc ccgtcccttc
      841 cgtgccccag gcggtgatga agttgccgtc gccgtcaaat ttttgaatgc ggtgattccc
      901 gttatccacc acaaagacat ttcccgaagg gtccacagcc agcccgccaa aacccattga
      961 attgaactcg ccgtcgccgc ttcctttctt gtcccatgcg gcgagcaggg ttccgctcga
     1021 gtcgaatttc agcacccgtg aattgcctgc atcagagatg taaatttcgc cagcctggtt
     1081 gaccgcggca ccatagggtg atttcaggta caggtctcct gctttgttta cggtgagaaa
     1141 aggcccaatt cccagatcag ctgtcggtga gggtggaatc ggggtttcag tcggtacggg
     1201 aggttcggtg gggctggcac atgcaccaag cgtgaatagg gttgccagga tcaaaatctt
     1261 caatgcagag ttcatttttt ttctccttga ataaagtctt tgcttgttca tctttcttat
     1321 ccgttataat cttctggcgt tatgaaaatt ctaaccgtac ttacttacta ccgtccacat
     1381 acttcaggac tgactattta tgccgagcgc cttgcacgtg cctttgtcaa acgcggccat
     1441 gaagtgaccg tgatgaccac ccactacgat ccatccctgc cgcttgaaga gatgatggat
     1501 ggggtgaagg tgattcgcat tcccgtgatc gcgcgtgtca gcaaaggcgt gatcgctccc
     1561 acatttgggc tggttgccac gaaactggtc tggcagcatg atgtagtgca gctgcatctt
     1621 ccacagttcg atgcgcccgg cgttgccatg cgtgcgcgtt tgttcggaaa acccgttgtg
     1681 ctgacctatc attgcgatgt gcaactcccg cagggatggt ttaatcgctt cgttaattcc
     1741 gtgctcgatt ttcaaaacga tatggcggga cgccttgcga atcacatcgt gacctacaca
     1801 caggactacg ccgatcattc tccctacctc tcgcgctacg cttcaaaact gaccccgatc
     1861 ctgccgcccg ttgagatgcc tgtccctgcg ccggaggctg ttactgcttt cgctgaaaag
     1921 caccgcacga aggaacttaa acctgtcatc ggcatggtca cacgctttgc ctctgaaaaa
     1981 ggcgtggaaa ttttgctcga tgccctgccc attattttga aaaaatatcc aaacgcgcag
     2041 gtcttgtatg cgggtcaaca ccagaatgtg atgggggaac aggcttattt tgaccgcctg
     2101 attccaaaga tccatgaata tgaaacctcc ggtcattgga cctttctcgg caatctgaca
     2161 cccgttcaac tggcggcgct ctatccaaat cttgatgtgc tcaccgtacc atcgctcaat
     2221 tccacggagg cctttggcct tgtgcagatc gaagcgatga tgaacggcgt tcccagtgtt
     2281 ccctccgctt tgccgggggt gcgcagaccc gttcaaatgc acggcatggg cgtcgtttca
     2341 gagatcggga acgcgcaaag tctggccgct tccattcttg aagtgctgga tacccctgca
     2401 aaatatcgcg gcgatgtgga tgggatcaag accacctacg atcccgactc ggtcgcgcag
     2461 gaatatgaaa aaatgtttga gcgtttgaag ggaagaacta cgggatgatt tcaaatccgt
     2521 gttggcggaa atgatcatcg aaagtgaaag cggtgcggat gttgtggcgt ttcatggtgg
     2581 caaaacttga gtaatccacc atgctgatct ggcgacgatt ccccgtaata acctgatcca
     2641 taaccccatt gtgaagtgac tcgtccagcc actcaatttc caaaaatgga atgatgtcat
     2701 tgtgtaaaat ggacagtgct tcaagtccaa cacggttctg gataagagcg atgctttcca
     2761 ctaaaacata attgttgctg gccagtcttt cgcctttttc aatgaggttt ttccaggtgt
     2821 ccagtgcaat ttgatacgat gggtcttctg gattgcaaat ggcaagaaag gcagatgtat
     2881 ccacatagat catgagtaag tctcattgag gtatttatcg tggtttttgg ccacgtctgt
     2941 ttttccttca atatctttga agtgggcctt cttgattttt tcaagacctt ccaatgcgcg
     3001 gcggcgtttt tcgtccattt gcgggtttac tttggaagca tcaacataca aggttacgct
     3061 ttcacgaacc aatgaggcaa cagatgcttt gcgtacccgt gccatttttt tcaaggtttt
     3121 tacctgttct tctgttagtt gaaccattac ccgtaccata aacgcctctg ctatcacttt
     3181 ttggaaagaa tgctatcatt atactatgtc gcaaaaagac tttctctttc tccaagtcag
     3241 ctcgctgccg tattttcgcg catttctgcg cgcggttgaa tcttcctact atcaagacct
     3301 gcccctccct gccccagtct atgacgtggg ttgcggtgac ggtcattttg cctccctgac
     3361 cttcgaccaa aaactggatg tagggctcga tccgtggcgt ttttccatgc gggaggcgaa
     3421 aaaatacaat gcctacaaat ccctcgtcga agcagatggg tcgcgcgcgc cgttcccttc
     3481 gaaccatttc gaaagcggat taagcaactc ggttctggaa catattccgc acattgatgc
     3541 ggtgctggcg gaaaccgccc gcgtactgaa acctggtgcg ccgttttatt tttgtgtgcc
     3601 aaacacccgt tatttttccg cgctttcgtt gacgaaggtc ttcggcaagc cgtatgaaaa
     3661 ctggttccgc aagatctccc gcgtccacca tgccgacgaa cccgacgtat gggaaaaacg
     3721 actggaagca gcggggttca aactcgagcg ctggtggcat tacttttcac ccgcttccat
     3781 gcgcgtgtta gaatggggcc actattttgg cctgccctcg ctggtggcaa aagcgcttac
     3841 cggccaatgg attctttccc ccacaaagtg gaatctcagc ctcacggaaa actatctccg
     3901 caaatacgcc tctcccgaac ccgtggaaga tggaaccttc actttttata ttgcaagaaa
     3961 gaagtaattg gcaactcaca attaccaatt accaattacc aattaccnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnncaa ttaccaatta ccaattacca attaccccca
     4141 tgtccttcga cgaaaaatac ttctcaaccc atacctacgc caatatcacc tttgccaaat
     4201 acagcatgta ctggtggtcg aaccgattct atgccatgct tgcgcggcgg tacgggcggc
     4261 gcggagcgcg tctgcttgag gtgggatcag gcatggggca tttggtgggc cagctttcgg
     4321 cgtggttcga agcgtatggc atggacctga atcactgggc ggttaacaaa tccaaagccg
     4381 atgccgaagc cgcatccctg caaactgcat cagcacagga cctgccatat caaaacggcg
     4441 cattcaacgt gatcatcatc aagcatattg tggagcattt gcccgaccct gcaaaagcca
     4501 tccgtgaaat tgcgcgcgtt accgaaaaag gcggcattct catcctggcc acaccgaacc
     4561 ttggttcact gctcaaaccg tggaagggca aaacatggat cggttatcag gaccccaccc
     4621 atatttcgct caaacaaccc gaagaatggc tcgagatcat tcgcggcgca ggctttgaaa
     4681 cgctccgcat tttttcagat ggtttttggg atgtgccata cattcgttat gttccaaaac
     4741 agattcaaaa gttattattc ggttcccttg gcggtctgca agccattacc ggattcgtct
     4801 ttctgccgat gcgctgggga gaaagcatca ttgtgatcgc gcgcaaacgc taatttcctt
     4861 ttgggtgaag atgtatgaat gaaccttctg ttctcgattt tgtaaaagcc attttcaaaa
     4921 actggaattc ttttcaatcc tttcttcagg ccctgttcaa gcggggggat gcctctcaat
     4981 taatgcagtc cgcagacgaa gcgacgattc aacttgaccc gcagcctgat cctgaacctg
     5041 tggaagcccc cgccccgaag aaactcccct ggcggacatt gctcgtgctg ttcctggcac
     5101 tcgcggcgca acagaccttt gagccgcccc gcaccagcac agccaacctg ggcgtcgcgc
     5161 tgtacattgc cgcctttggg ctggccgtgt gggcctacct gcgcggcgaa tggacgatga
     5221 ctcccgtttc tcagatcgtg cccggtgtgg aagatgccag catccgccgc ggctcgttcg
     5281 tgctttcggt catcttcagc gccgcaacct tttggatgat gggcggcaat aaattcactg
     5341 ccctgaatct cacactgtgg gtgcttgcca ttgtgttttt cctgcgtgct ttctggctta
     5401 atgaaccgcg cagcgccgca tcgtcaaact tcgatctgaa atccatcttc accagcagcg
     5461 aatggaatat ccgcatcaca cgcagtgctt tggccgtttt tgtgatggcg gtcatcatca
     5521 tcttcttccg cacgtatcgc ctgtacgatg tgccccctga aatgaccagc gaccacgccg
     5581 aaaaattgat ggatgtgtac gacatcacgc agggacagtt ctccatttat tttccgcgca
     5641 acaccgggcg tgagccgctg tatatttatc tctgtgcctt gttcgcagag ttgttcacgg
     5701 gtacctcctt cctcactttg aaagtggtgg cagtcatcgg cggtctgctg accctgccct
     5761 acatttacct gcttggaaag gaattcggca gcgaacgcat tggtttgctg gctgtgctgt
     5821 ttgcaggcat cggatattgg cccagtgtga tcgaacgctt tggcctgcgc atttctttct
     5881 acccgttgtt tgctgcgcca actttgtatt acatcatccg tgggttgcgc cgccagaacc
     5941 gaaacgattt cattcttgca ggcattgcct tgggcatcgg tttgaacggc tacaccccat
     6001 tccgcatcat gccatttgtg gtggtggcct tgttcggcgt gtacctgctg catgcccgtg
     6061 actggcaggc ccgcaaacaa tccatcatct ggctggcttt gctggccttc acttcttttg
     6121 ttttcttcat cccgctggcc cgttacatga tcgagttccc ggacatgttc gtgtaccgtg
     6181 ccttttcaag gttgagcacg gtggaacgtc cactgcctgc gccgatctgg caattgatcc
     6241 ccttgaattt gtggaatgct gcaaaggaat tcaactggta taacggcgat atctgggtgc
     6301 attccattcc cggtcgcccc gcattggaca ttgtctccgg tgcgctgttc ctgctgggtg
     6361 tcagtctgat gcttttccgc tacatccgct cacgcagttg gatggacctc atgctcctgc
     6421 tggcggttcc attcttccag atgccttcca ttctttcgct ggcttttcct gaggaaaacc
     6481 cgtccttgaa tcgaacgggg gctgcgatcg tgccggtctt cctgctggtt gccatcgggt
     6541 tggatacttt gttgaacagt atcaacctgc gggaaaaagc gaacgaagaa tccatcatga
     6601 gcgaaaatcg ccgcgcaatg ggacatcgtc cgcttttggc gggaaccatc ttcgttttgt
     6661 tattcctctt ttcctttact caaaattaca accttgtctt ccatacttat tacgaccaat
     6721 accgtgcttc tgcgtggaat tcatctgaaa tgggtgcggt gatgaagggt ttcatggagc
     6781 gcggtggttc tgccgacaat gtgtggatcg tgcctttccc attctgggtg gatacgcgtc
     6841 tgccgccgtt ttgggcgggt gtgcccggca gggatattgc cattgccccg gataacatcc
     6901 cagccacggt ggaactttca ggcgacaagc tgttcatgtt cacactggat gacacggtga
     6961 ccatgtctga attaaagcgt ttgtatccgc agggcacatt atctcaattt cattcggcag
     7021 ttcccgcgca cgatttttac gtgtatcaga tccctgcctc gcaataaccc gcagactcca
     7081 atgtcggaag gcattggagt ctgttttttt aatcatgcaa aacgaaccat cccatcttaa
     7141 agcatttggt tttccgtggc ggtctctggc agcgcttgtg ctggcattga ccgctcaact
     7201 catgttggaa ccgcctgcaa agatctgggt gggcatcgtg ctttacattg ccgcgattgg
     7261 gctggtcgta tggtcgtttc tgcgtggtga gtgggtgatt gcatggatcg cagaagatat
     7321 ttaccacgga gatactgagg cactggggtt taagagattt ttctccgtgg ctctgtggct
     7381 ccgtggttca aaacagattt tattttttct ttccctgccc ctgcttggcg cggcgtttta
     7441 tttttttcgc ggcaatcaat ttactgtttt gaatatttcg ctttggctca cgggtttggt
     7501 tttgttcatt gttgcgtttt ggagtaccac cgcccgtacg gccgacttca gtcggctagg
     7561 cgtgaaccga ctgaagtcgg ccgtacagaa aatggattgg gaatggctcg tgctgctgct
     7621 tgctgttttt gtcttcgccg ccttctttcg gttttatcgt ttgaacgaag tactggccga
     7681 gccatttagt gaccatgccg aaaaactgct cgatgtgtac gacatttcga aaggcaacac
     7741 gtccattttt ttcatccgca acaccgggcg tgaaggcctg caaatgtatt ggacctttct
     7801 tgttgcaaaa atattcggca cgggtatttc gttttttagt ttaaaactcg gcacagccct
     7861 gattggtctg ttcacacttc cgttcgttta tttgcttggc aaagagtttg gaaaccccgt
     7921 ggtcggtttt tttgccatgt ttttatttgg gatttcctac tggatgaacg tgaccgcccg
     7981 catcggattg agattccccc tgtatccgct cttcgccgcg ggcacgcttc tttttctaac
     8041 ccgtggcctg cggactgctt cgaggaacga ttttctgctc tgcggccttt tgctcggact
     8101 cggcctgcac ggttacaccc cgttccgcat catgccgttc gtggtgttga ccgcgttcgc
     8161 gatttttatt ttgcacaaca attcaaaggc gattcgggtg caggcggttc aaggcctgac
     8221 gatcatcgcc gtcaccgcgc tgatggtttt cctgccgctt ttgcgctatt ggctggaata
     8281 ccccgaggtg ttcggcttgc gtgccttttc acggctcgac tcggcgggaa cttttggttc
     8341 gctgatgggc gtcttctttt caaatttggc caacgccctg ctcatgttca atgtggatga
     8401 tgggaacatc tgggtgaatt ccctgccgca ccgccccgcg ttggatgtgg tgacaggcgc
     8461 attttttgtg atcggtctgg tgttgttggg tttgcggtat ctgcgccaac gggattggcg
     8521 tgacctgttc ctgctggtct tgattccgct gttgctgatg ccttcggtgc tttcgctggc
     8581 gtacccggaa gagaaccctg ctttgaatcg tgcgggcggc gcggcagtgg ctgcgacttt
     8641 gattgcggcc ctggcggtgg aaggcctggt tgtaggaact gtccgtggtg ttgaatcagg
     8701 tcagggtcca gaaaagaggc ggggtttcat cctccatatt ttggtgggag tgttgctggc
     8761 ggctgcgtct tttcagaatt acgaccttgt cctgcggcaa tttgaccgct atttccgcat
     8821 tggcgcctgg aattcttctg aaatggggcg ctcgatcaag ttttttggcg atgtgtatgg
     8881 gcgcaccgat acggcctgga tcgtgcccta cgagcagtgg gtggataccc gcctgcccgc
     8941 cctgtggatg ggtgacccca accgcgattt tgcgctgtgg ccgagccagt ttgccgatac
     9001 gctggcaatg cctgcgccga aaatgttcat cttcaatctt cgcgatctgg acactaaaaa
     9061 tgcccttaag caactctacc cgaatggtac gttaagcaga tatacttctg ccacgaccgg
     9121 caaggattac atgattttct ttgtggagga atgatgtcgt tgtgagttgg aggccgtgcg
     9181 gactccctct tttcactgac atgaagtttt atgactgaaa aacgcgcatg gttttatgat
     9241 cttctttttg tctgtatcct gctgatcgca gggtttttgc gattgaccgg ggtggagtgg
     9301 ggagagggtt accaccagca ccccgacgaa ctgtttttgg tgggtgtgct cgataacctg
     9361 cgggcgcaca cctgcgccga tgccgtaacc ccggtggacc tgtgcaaacc cgaagaccaa
     9421 cgctggatga cccttgggga atatttcgat acggccactt ccaccctcag cccctacaac
     9481 cgcggacatg ccttcttcgt ctatggcaac ctgccgatga cggtgacccg cattgccatg
     9541 gaaatgaccg gcaacgatga cattggcaaa tcgaagttct ttgcgcgcca attttcggcg
     9601 ctggccgacc tgttcaccat ttttattttg tatttgatcg tttcaaattt atatgggcgg
     9661 cgtattggtc tgtttgcggc tgtattttcc acattcgccg tgatgcagat ccagcagtcg
     9721 catttcttta ccaccgacct gttcgtgaac ctgttcatgt tcctggcgat catgtttgcc
     9781 atgggcattg ttgaaaaacg ggatttgggc atcggcaaca acgaatcgga gactttgcaa
     9841 ttaacgtcat ctgatgccgg gttttcaaat tacctgttgc gtttggtgcg ctcgcccctt
     9901 ttcctgctca gcattggttt tgggttcgcg cttggcatgg cgatggcttc caagatcaat
     9961 gcggcggcgc tggcgatcct gctgccgggg gcatttgctg tccgttttct ggtgcaggac
    10021 aaagacaaaa aactggccgc tgattattgg tcgttgatca ctgtcttttt gatcgcaggc
    10081 ggactcgcca ccatcatttc cttccgtatt ttccagccct atgccttcaa tgggctgggg
    10141 ttgaacgatc agtggctctc gaacattgct gaacagcgcg tgcaagccaa tggcgatgcc
    10201 gacctgccct ggaacttgca atgggcacgc cgcacacatc tgtattcctt caccaatctc
    10261 accgtgtggg gattgggtct tccgcttggc attctggcat gggtgggttt cctgctgatg
    10321 ggttggcgca tcctgaaagg cgagtggcgg cacctgctgt tgtgggggtg gaccgcattc
    10381 tacttcctct ggcaatcgct gcaattcaat gccaccatgc gttatcaact gccgatctat
    10441 ccgctcctgt gcatgatggc cgcctgggct ttgtttgaat tgccgaaatt gcgcggcgaa
    10501 accgggcgtt ttaaattatt cgaagtcttg gtcgccacca tgggtgtggt ggtgctggtg
    10561 acaacgacca tctgggcctt tgccttccaa agcatttaca cccgcgacga accacgcatg
    10621 gccgcctcgc gctggctgtt ccaaaatgcg ccgggcccgg ttaatgtgaa gatcaaaatg
    10681 gccgacaaca ccatctacaa ccagccattg cctgtgccca tggatatggc gattcaggca
    10741 ggggcgccct tcacaatgac cttttccgcc aatcgtgatg gggatatctc tgaaatcgtc
    10801 tttggtcatg ccattgatct tgaatcgcct tcgacgattg tcctgaccct ggcatccgcc
    10861 tctgcaccgg acctcgtgct ggcgcgggcc tccgaaaaag tggattactc gcagacgaag
    10921 gatccgcgcg gccccgccgt ggaatttaaa ctggatgaga ccgtcccgct tgtcaaaggt
    10981 caggcctatc ttttgaagat cgaaacgctc ggacatgcgc tcactttgag cggatcggcc
    11041 attgccaacg aaacaggtta cgactggggt ctgcccttcc gcattgacgc ctacgatgcc
    11101 ttcggcggca tctaccgcgg cgacctgacc ctcgaagtat attgggatga caaccccgat
    11161 aaggtcaacc gctacgtgag catgttgagc atggccgatt acatcgtcat tcccaccaat
    11221 catcaatatg cacagatcac ccgcctgccg gagcgctatc cgctgacgac ctattactac
    11281 cgtaaattgc ttggctgccc cgaaaacgcc gatatcatcg aatgctatcg caacgccaaa
    11341 cccggtcagt atgaaggtgg tctcggtttt gaactggtga aggtttttga aacctacccc
    11401 cagtttgggc cgatcatcat caacgatcag gcggcagagg aagcctttac tttttacgac
    11461 catccaaagg tgttgatctt caaaaagacc tccgatttca acgcggataa actatctgca
    11521 atgctcaaca ccgtggacct gacaaacgta gtccacctca cgccgaaggg cgccgataac
    11581 tacaagagcc tgatgctttc gaaagagcag tacgcggcac agcgtgcggg cggcacatgg
    11641 tcgcagttgt tcaattatga atggcttcaa aacaaatacc ccgtgatcgg gcttttgctt
    11701 tggtatgcat tcatcttcct gcttgggctt gcggcctatc ccattgtgcg gctggctttg
    11761 ccgggcttcc gccaatattc atatccgctg ggacgcatgg cgggtttgtc tttgctggca
    11821 tggatttcgt ggatgggcgg ttcgctgggc gtgccgtaca cacgcggcac catcggggcg
    11881 gcctttgtcc ttgtcactgt ggcaggcctc gggttgtggt ggattcgcaa agcgcagttc
    11941 aaagatgagt gggattccaa tcgcaagttc ttcacgttgg tggagatcgt tttcgctgtc
    12001 ttcttcctga tcgatctgct catccgcttc ggcaactctg acctttggca tccctcaaaa
    12061 ggcggcgagc gcccgatgga tttctcgtat ttcaacgcca ttttgaaagg gacgagtttt
    12121 ccgccgtatg acccgtggtt cgcgggcggg tatatcaatt actactatta tggttttgtg
    12181 ctggcgggca cgccggtgaa attgctcggc attgtgccat ccattgctta taactttatt
    12241 ttgcccacct ggtttgcgct ggtggctgtg ggtgcgtttg ccatcggttg gaatttgctg
    12301 gaaaaagagg aaggtgcttc ttccaacttc tccgcacagc attcatcctt gctttccggt
    12361 ctttctgctg cggttctgtc tgttttgctt ggcaaccttg gcacgatcca gatgatcttt
    12421 caggctttgc agcgtatggc ggcaccgggt gggatcatta ccgatgccac ctttgtgcag
    12481 agatggatct gggcttttca gggattcatg atgtcatttg gcccgggcgc ttcactgccg
    12541 atcgggcgcg gagaatggta ttggaatccc agccgcgtga ttccgccggg cccgggcaat
    12601 gagatcaccg aatttccgct tttcaccttt ttgtacagcg atttacacgc gcacatgctg
    12661 gtgatgccgc tggcattgtt tgtcattgcg tgggcgctgt cctttatcaa atcgcgcgcg
    12721 aacctgcgcc gtgaagaatg ggctgcttcg ttcctgttgg gcgccatggt gctgggagcg
    12781 gtatacccaa ccaacctttc tgatttttat acctatttgc tgttggcgtt ggtcgtgctt
    12841 gcgttcgtct tcctcttccg cgctgaagtg agcggactgc ggttgctgga agacctgccc
    12901 gatgcagtga aacgcatcgc cttgatgctg gtgagcatcg gtgcattgac cgtgctttcc
    12961 ttcgtcatgt atcaaccgta tcgcagtgca tacagtcaat cgtatggcgc actcgacccg
    13021 tggacggctt cgcatacacc cgtttcttcg tacctcaccc attggggatt gttcctcttt
    13081 atcatcttcg catggatggc ttgggaaacc cgcgaatgga tggcgcgcac gccgctttct
    13141 tcgctggcaa gcctgcggcc gttcgcaatt ttgatcgaac ttgcgttggc gggtattttc
    13201 gtcgttctgg tttactttgc ctacgaaggg atctgggtcg gttggctggc attgccgctg
    13261 gcagcctggg caggcgtttt gctcctgcgc cccgatcaac ccgacgaaaa aaggctggtc
    13321 ctgttcctca ttggcacggc attgatgatc actcttgtag tggaattgcg aaccgtgcgc
    13381 ggcgacattg gccgcatgaa cacggtcttc aagttttaca tgcaggcctg gctcatgctt
    13441 tcggtcagtg cggcggcggc cctcggttgg atgtttagcg atgccccgcg ctggtccgct
    13501 ggatggcgca acaccgtgca ggtgattctt ttgacgctgg tctttggcgc caccctgttc
    13561 accttcaccg ccacctcaga caaggtgaca gaccgcgcct cgaccgatac gccgcgttcg
    13621 ctcgatggct tgaactacat gaattattcc acctacttcg atcacggtca aaacctgacc
    13681 ctgaacgaag attaccgcgc catccgctgg atgcaggaca acgtgcaagg ctcgcccgtc
    13741 atcgtggaag ccgcacctgc aggcaggcaa tacgaatggc attcacgctt caccatttac
    13801 acaggcctgc ccggtgtggt cggctggcaa tggcaccaac aacagcagcg cgtgttgttt
    13861 tcgcagcaga tcattgaccg cggcatcgaa gtggatgcct tctacaactc gctcgatcaa
    13921 gactctgtgc gaaaattcct tgcgaaatat gaggcgcgct acatcatcgt cggacagttg
    13981 gaacaggcga agtacatgcc cattgacccg ggcattccaa acggcctgat gaaatttgac
    14041 ctgtggaatg gcacactctg gaatgaagtc tatcgcgacg gcgacaccgt catttacgag
    14101 gtgatccaat gatcctgcac atcacatccc gtgctgaatg gagcgcggcg caggcacgcg
    14161 gcgaatatat tgcgcccagc ctgcagaccg aggggttcat tcattgttcc actgacaaac
    14221 aggttgccca tgtggcgaat gccttttatc gcggacgaaa tgacctggtg ttgttaatga
    14281 tcgacgaagc aaagctcaag cccgagctta agtgggaacc gcctgctgga ccacccgccc
    14341 cgggcatttc cgaatccgat tcatttccac acatctttgg gcccatcaac ttaaccgcag
    14401 tcgcctctgt gctggacttt gaacctgatc ctgcatctgg aacattctcc taccccaagg
    14461 catgaccgaa ccaaccgccg cccttcgtat tcgcccggcc catgccgccg attccgcgct
    14521 tgctgcaaag gtgatttaca tgtccatggg cggccttgcc gattatcttt tcgataacaa
    14581 cgggcaggcg atccagacga tattggcaga gttgttcaca cgcgatgccg ggcggtttgg
    14641 ctttcgcatc gccatggttg ccgaagatga aacacagcca gtgggcatgc tgttcgcctg
    14701 tcctggcgca cggctggcgg gtttcaatat ggcttcgttt cagcatttct tcccggtcat
    14761 ggggttcaaa catgcgctaa aattcatgtg gcgtggtgta accctgcccg gcggccccga
    14821 agctgaaaag gacgaatatt acgtcagcaa tctcggcatc ctgcccgagg cacagggacg
    14881 cggtattggc acatctctgc tggcatatgc cgaagagacc tgccgtgctc atcaactctc
    14941 gaagtgttcg ctcatcgtgg gtatgcataa tgaaaatgca ttccgcctct atcaacggct
    15001 cggttaccaa gttgtcgaaa ccgctcatgc gcccaacgaa caccttgcct attaccgcat
    15061 ggtcaagcac ctgaactgat cgcagactct ccacacattg gatctcacaa atgaccgcat
    15121 ttatcgcctg gtatttcatc ctcacccttc tcggctggct gacctttccg ctggctcatt
    15181 ttcttttccc cgctttaaaa gatcgcgggt acaccctttc acgtgccgcc gggcttttga
    15241 tttgggggta ttccttctgg ctgatggcct cccttggtat tgcacaaaac gatctgggtg
    15301 gaattctgtt ggcgttgatc gtgctggcct cattgagtgc gtgggtattg gccaatcaaa
    15361 ggtctgagat cattgatttt ttcagaacgc atcgttcact ggtcatcacc acagaggtct
    15421 tgttcctggt cgcttttgcg ttcctggctt ttgtccgctc ggccaaccct gaattgaccg
    15481 gcaccgaacg gcccatggaa cttgcattca tcaatgccat tctgcgttcg cctgttttcc
    15541 ctccacaaga tccctggctt tccggatacg cgatttccta ctactacttt ggctatgtga
    15601 tgaccgccat gcttgcaaaa gtatcgggca tttatggcag catggcacac aacctgatga
    15661 ccgcgcttgt ctttgcattg ggagcgatcg gctcctatgg cattttgtac aacctgctat
    15721 cacatggaca tgaaccgaag aagaccgaag acggcgatat ggataccgct ggctcgcatc
    15781 gttcttcttt ttccatttat ggttttctat ccctgctggc cccgcttttt cttttgctta
    15841 tctccaatct gggcgcattg ctggaagtga tgcatcaatg gggtctgttc tggaaacagg
    15901 attacatcag cggagaatac accagcgcat tttggaagtg gatggatatg aaggagcttt
    15961 cacagtctcc cattttgcca ttccatgcgc cgccgcttga ccgatacctg tggtggtggc
    16021 gttcttcacg cgttttgcag gattatgaca tgctcaatcg tccgctggag gtgatcgacg
    16081 aattcccgtt cttctccttc ctgttgggtg atttacatcc gcatgtgctg gcgattccgt
    16141 tcggtttgct ggcgattgca gcagctttaa atctcttttt aggcggctgg cgcggtgaag
    16201 tcaaattacc gttgggcgca cgtttgcaga tcagcggacc ggggttttta ttcacagcct
    16261 tgttgcttgg cggattggcc tttctcaaca catgggatat tctcattgcc gcggctttga
    16321 tcgttgcttc gtatgtgctt tggtgtgcaa atgaagaagg ctggcgatgg gaccgccttg
    16381 aagatgcgct gttgtttggt tttccgctgg gcattctggc gattttgatg tacctgccgt
    16441 tctatgtcgg cttttcatcg caggcgggcg gcattttacc caatttcatg taccccaccc
    16501 gcggcattca tctgtgggtg atgtggggca cactgcttgt tcccattctg gcttatgtct
    16561 tgtatctgtg gcgcggcgaa aaattccctg ccaattggaa actcggggtt tccctcgggc
    16621 tcggattcgt tgtgttcttg ctggtctttt cgtggctgtt gggggtgatc ggttccatcc
    16681 ttgaaaagga ttttgtcaat ggattcctcg cctcacaggg aatgacagcc agtcagttct
    16741 ttggcgctac actgatgcgg cggttgagtt acattggcgg gctcatcacc atgcttgcgg
    16801 tttttattcc ctcactggct tttctgtttt cgcaaaagca acaaggtcag cccgaagccg
    16861 aattatcggc accagttgaa aataaccgca aaccagagac ccagtttgtt ttgctgatcc
    16921 tcatccttgg cgccattttg atcatcaccc ccgatttcgt atacctgcgg gacaagttcg
    16981 gctatcgtat caatacggtg ttcaagttct attatcaagc gtggatgtta tggagtttgg
    17041 cggcggcttt tgcggcttca cgtttgttgc aaagcctgcg aggcgcggcg aatgtagcct
    17101 tccgggtggc gctggtgttg gtggtctcaa cggggttgtt gtatcctgtt ttggggttga
    17161 tcaccaagac gaacaacttc aacccgccgt atggctttgc ccttgatgat tttgaacggg
    17221 tcatccgtga gacaccggaa gaggcggctg cgatcaactt tttacgcact gcgccggatg
    17281 gcgtcgttgc cgaggcgatc ggcgattcgt acaactatcc atttacgcgc atctccactc
    17341 atactggcct gcagacggtg cttggctggc ccggccacga agatcaatgg cggggcagtt
    17401 atattccaca aggctcccgc cgcgatgata tcaaccgtct ttacaccacg gtccgctggg
    17461 aggaagcctt gcagatcatt gagcagtaca acatccgcta tattttcatc ggcacactgg
    17521 agcggacctc gatgaatgtg agtgaagaaa aattcattgc ccaccttaaa ccgatctttc
    17581 aacaaggcgg cacggtcatt tacgaagtgc cagtgctgga gaaataaaaa tataaaaaaa
    17641 gtccccgaac tctcggggac tttttactgt aattagggtt ttgcaacgga gaatttttct
    17701 ttgccgatct tgtaattgcc gctgtgtttc agcacctggt ccacggcatc ggcggggaca
    17761 tctacaaaag aaaccttttc ttcaatgcga attttaccga tggtgtagcc ggggatgttg
    17821 gcatggaagg cgatctgccc tacaatgtca tttgggcgga tgttatgggc cttgcctttg
    17881 ttgatcttaa ggcgcaccat gccttcctcg tgtgagtcgc ggcgtgattt tttatcaccg
    17941 aattcacgct tgctgccttt ggctgcagag cctgcaccaa actcgcgctt gccgcctttt
    18001 gaatcacggc ggccaaattc acgctcgggc ttgcgggtga agtcggtttt cacatcagac
    18061 acttcagcaa tgggacgctg tttttcgtcg gcgcgggcga tcttaatggc ggcggcggcg
    18121 atgttcatta cgtcatgccc ggcttcgatc agttcctgca ccatttcaag ttcacgtttg
    18181 tatcgcgcac ggccaagcca gatcttcagg ttttcgacca ccagagattc gcggtggcgc
    18241 atgatctcag aggcggtcgg cagttccatt tttgtaacga gttgtttggt gagcgcttcc
    18301 acttcacgca tgcggcgttt ctcgcgcgga gagatcaggc tgatggcgat gccggttttg
    18361 cctgcacggc ctgtacgccc aatgcggtga acgtacactt ctgcatcatc gggcaggtgg
    18421 aagttgaaga catgggagat gtcgtcaata tccaacccgc gtgcggccac atctgtagcg
    18481 accagcacct tcaattgatt ggcgcggaaa cgtccaagca cacgctcgcg ggcattctgg
    18541 tcaaggtcac catgaatggc ttcggcaggg atgccgcgga tgaccaattc attggcgagt
    18601 tgtcctgttt cggcgcgtgt gcgggcaaag atcagagcgc tgtgaatggg ttcgacttcg
    18661 aagagtcttg tgagggcgtt gattttgtcg ttgtcgtgga caaggtagta gcgttgttca
    18721 atggctgcag cggtgagcgt gcttcttttg atgacgacag attgagggtc acgcatgaag
    18781 cgatttgcaa gggcgcggat gcgcgggggc atggtggcgc taaagagggc ggtctgcctt
    18841 tcggagggag tcatcgaaag aattttttcg acttcttcaa tgaaacccat gttgagcatt
    18901 tcatccgctt catccaggac gacggtcttg atctcgttta agatgaggac tccacgctca
    18961 agcaggtcgt ttaggcggcc tggtgtaccg acgacgatat caacgccgcg tttgaggctg
    19021 ttgatctgcg ggctgtacgg ttggccgcca tagacggcaa gtacgcgcag gttgaggtgt
    19081 ttgccatatt cggtcatcga atcggccacc tgcagggcga gttcgcgggt gggggcgagt
    19141 accaatgcct gcggattttt ttgatgttga aagttgttca gaatggggag ggcgaaggcg
    19201 gctgttttgc ctgtgccagt ttgggcttga ccgatcacgt caacgccggt gagcatgagg
    19261 ggaatcatgc ctgcttgaat gggggtgggc tcggaatagc caagctcagt gatggcctgc
    19321 gtgatctctt cacggaggtt gagggaggaa aaatcggttg tcatctttgt cctttagatc
    19381 tgtggtcggc tggtccatga tttggatcgg cgaccctaag ggattgctct gatgactccg
    19441 ctgtaaattt cttcctgtca acaaggtttg cttttgcagg caaacccgcc caacaaaaaa
    19501 gcccgataaa atttcatcgg gcattcgttc agagtgatca aaacaatttc ccagaaaatt
    19561 cgccttccat tgaaggcggg aagagtttac cacagttttt gagattcaag ccctgatacg
    19621 cagaggcgcg aactttgcta tactttgtgt atggctgcta aaaatgttca cctcaactgg
    19681 aatcctgacc agcaattcac ccttcaggat aatgatgggt accagctcgt tatgaaaaag
    19741 ccaaagggag tcagtgcttc tgacctgctg cccatggcct tgatcggatg cgcttcgcac
    19801 gatgtagtgg agatcctcaa taaacagaag caggatctac gtcaattgaa tgtgactgcg
    19861 gaaagtattc aagatgagac cccaccatgg cgttttcgaa aaatccacat tcattttcag
    19921 gttgtcggca aaggcattga ccctgaaaag gtgcataagg cgattcaact gagcgaggaa
    19981 aagtattgtt cggtgtatgc cacattgaaa gatgcggtgg agatcatcaa cgactttgaa
    20041 gtggtggaag cgtaaatttt acgggtggtt tgaaacaagt atttgaaaca gatgccgctt
    20101 tctggcggtc gaatgcgttg agcgggatat aaacagtttt tgaatcagat gcgttcggct
    20161 agtttgtcta atatagagat ggttcccatc cccagcaaca tcagcaggcc gccgatcagg
    20221 atcggaatgg atacggcagc caataatacg gtggtaaaaa aggtattacc tgtctgcgga
    20281 agataccaca ccgtgtggtc ctcaagaatg gtatagtaga ttttttgcgt tgaaccggga
    20341 taagcgggaa ccccgatcag gtttgtagat accccgggcg cttcaatgtt tggtgaggtt
    20401 gattcatcca tctcttgtag aagagaagcg tcgggctttt catctgccag ccatcccaaa
    20461 ccatcgtctg gaatggtgtg cgaaaaataa ttcccttttt ctgtttcaat gatgatgcgg
    20521 ttgtaaatcc ctcccgaaat ggttctggcg gattcgggtg gatcgggcaa ctgcacccaa
    20581 ctttctttgg gtgagaaaaa ggtataggtg aggataagcc caagaatggg cagaacaaca
    20641 caaagaaaga gggagccgaa gatcactcca tttattgcca gtttgcgctt cgcagaatcc
    20701 tgaatgcgaa gaagtagacg gttgattccc actcccgacc agaaacacaa caaaattaaa
    20761 acggccttcc aaagcgcatc agtcaaccct agaaagaaaa ctcgaaactg aaatgacggt
    20821 tcgagttgtt gaagcgcatc ttgttgaggt tggagtgtga aaaagcggat gaggggcagc
    20881 acaatcatta aggctgcaga gagtgtaatt agcaccaaaa acgggaagcg ctgcttaagt
    20941 ttcataaggc gcgattatat ggatgctgtc ttttttttca ctcccctgtt ggtacagtct
    21001 ttgaaaacaa aattgctccc acaacactca gcacccataa tcatctgtcc tcttatatca
    21061 aaaacactgg gacatccatc ccagtgttct ctcgtttatg cttggctatt tacttctgcg
    21121 tgcctttgcc gcaattgacc tgaccttctt ttgcttttca acaaccacct ttgctcgttt
    21181 tcggataagg gaacgcaaag ccgcgttgac actttttgag tcagggaaat gtgccttcac
    21241 atccttatcc aacaggatgg cactttcttt ggggacaaaa tgccgcatgg tgacagttcc
    21301 gtcctcatgc atcacgcgga cagaataccc tgcttgcaag gctttgacat atttcccgcg
    21361 tacacctttt ttgccttcca aattatattc aggcagcata tcttcagttt gagaagtctt
    21421 attcgccttt ttcatatcgt tttctttcgg ttggagttgc ttttcgacaa ctgatgatac
    21481 ggatgatgat ggtttctttc ttttcttcac gttccgtgtg gactacaagc aaaatacgat
    21541 tgttttcgga aaggccaata ctgatgaagc gctcttcctt ttcggaatgc agttcatccg
    21601 cgaaggtcac aagaagtggg tctacaaata ttgtgcgtgc ctcatcaaaa ctgactttgt
    21661 gcttctcaaa attggtacgc gctttgtttt catcccactc gaatatgatc ttcataccca
    21721 tattataccc gcgcctctat tccgcaaatg gactagcctt taggagaggg tggggggaga
    21781 ggcccaccct tcaaatcaaa acacagggac acccatttaa taatgttttt tggtttcccg
    21841 tcaaactggt tgacgatgta tcttagcttg atacctcttg aatacttttt caagatgata
    21901 aaatcaagtc gtttactcgg atttcaaaag gacaaataat ggtagacatc ttgcagaata
    21961 tcgggtcgac tctatgtata ttgggccttt gctttggccc cttccttttc attgggtttg
    22021 ggcttccagc gattgggtac attagtaaac gcaggaacgc ccgcaaacaa aagcggagca
    22081 accagcagca ccgctcaagg ttcccatatc ggcttgttgc gtatgctatg ttgggggtga
    22141 ccctgatgac agtggccatc tgcatttact ggctcttttc agggacgttc gacatttcga
    22201 acgatcttgg cggggttgca ggaatcatcg ctgttccgct tttgttcacc attgccgcct
    22261 tccgttccta cattctacaa aacatgccgt ccgcgaccga ggcagtcgag caggatccac
    22321 gaccgcccgt gttatatctg cgttccttcg accaggagat gctgaaattc gtacatctaa
    22381 aggatgaaga aaaagccaga tataacacct tcctcaatta cgccggtgat gtcaaccttc
    22441 cgccctttga acaagcgatc tacacggcat tgaacagttc cgcattattg acctttggct
    22501 ctgtggagtc tgaggatgtg accttcgaaa aatatttccg cggcgaggtg atggaacgca
    22561 tcggaccctt cgtggcgctg ggcaacccca ttgacagcct gcctaccgag ggagcctttc
    22621 gcgattacca gatcgatgaa cgttggaagg atgttttctt caaccattcc gaacgcgccg
    22681 cgtgtatcct tatgcaatta ggcagctcgg acaaccttca atatgagttg accgcgatcc
    22741 tctcccgccg tatttcgcat aaattgttca tcctaactcc accccaggaa aatctgaaag
    22801 accgttatgc ctgggtacgg aagtatatcc tcagggaaaa gcctgcaaac tggcaggcat
    22861 tctccgccat cctccaaaaa aatgggtttc ggattcccga cgaaccgctt gg
//
LOCUS       DAOQLC010000070        22623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_780_length_22623_cov_22.142237, whole genome shotgun sequence.
ACCESSION   DAOQLC010000070 DAOQLC010000000
VERSION     DAOQLC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_780_length_22623_cov_22.142237"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PL000_12985"
     CDS             <1..476
                     /locus_tag="PL000_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89862.1"
                     /translation="TNEYLVEGLDDSDSSALSASFDAEVEDPAGGCLILKDTHVRSMG
                     NIHTPARTFPEWHIPSLTNVVAMFSDDPAAEDILLEVWEDHQTPHKVIIYSGVYVIEG
                     TIYSEEEAPPDFMLHAFAPIENLKITNQVDKKAEPIHAKWGVVNSTMMQGYGVEK"
     gene            691..4734
                     /locus_tag="PL000_12990"
     CDS             691..4734
                     /locus_tag="PL000_12990"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC89863.1"
                     /translation="MFARKLFAGVLLAGLVFSQLAVSSAAAKACNQAQFISDLTVPDG
                     TSYTVGTTFTKTWRLLNIGSCTWTTSYSLVFVKGDQLGAPASTNLPVEVAPGQMVDLS
                     INLAAPQTSGHYRGYWALNDPSITAGAFGIGTSGTDPFWVDINVLGTSAVIYDFVANA
                     PYAEWKSGSGILPYPGASGDNRGYSQLIDFPRLENDSIDSQPGLLTVPQNKYNGFIQA
                     TYPEFLVSKGDRLQTLVNCEYGATGCYVTFGIDYVTSTGSVKGLWSWKEAHDGRFYRA
                     DIDLSILAGKKVKFILKLLSVGSASGDRAVWGSPRIVRSSVETPPAPPSTLTPLPPLT
                     PTLTPFATAPPISAAGCNKAAFVADITVPDGTLFSPGSAFTKTWRIKNSGSCGWTTAY
                     SLTYYSGERMNAPTSLPLPKAISPGATVDLTLNMTAPASPGKYRGYWILKNAAGELFG
                     IGTNASMPIWIEINVTGSPALNTGYDFAANACSAEWKSGAGILPCPGTTTDKNGFILK
                     MDSSQLEDGTTNSNPALLVGPQNKYNGYVQGIYPTMTVQPGDHFRTTVGCEFGTSCYV
                     AFKLDYMTSTGTITNFWTWREQNEGRPNNIDLDLTPLVGRSVRFILTLSANGSATNDR
                     ALWSAPVIIRTNLTPTFTPTSSPTPQVNDWLTYSNSDHGFQFKYPKSSQINEMLENSI
                     SMNLPIAAGTNLSQKSLQVIAVQNANPCSSYLGRSSMLSSSETVLINGLTFLKETGGD
                     SAAGQLHQWVAYSTLRGDTCVTFDMILHIINLGNYLTPPPAFNETAEISVLKDVVSTF
                     TWLGATPTPTFTPTPFGYDWPVYFNSKYGFQIQYPKEATLAIQNDNAAHLNLPFAAGT
                     NLKEKIFDIFIREDLQTCRSIIASSSMLVTSETVTINNTTFLKEVGEDGAAGSLYQWV
                     AYSTLKNNACISFEMRLHSISPGVYETPPPIFDYAAETSIIQNMLATFGWSPATSTPT
                     PTAIPTSAATLSAIFEAMNTRDYGTLKASMGETFAIAYWQSQGTSSTPDEAIEQLKTY
                     YLASPKALSSDPNRDLTTLLGGANPYALMGLDPTKSQALLVSGLGPQGIDEAILYMTQ
                     RADGTPYWFGLLTAKGGFVSFTPYAVTLIATGGVLDVYSAPGISNPIVASFPFDGINI
                     KRTGPTQLEAGTEWVEVIRPDGGTGWVNSHYLTEYIPRDGFCADARIAPIIAQLKNSV
                     TLSDGTLLGSLTSPKHGLNVNYWQYSNTVNYTSAAIQTIYTDPQAIDWGSGGASGITD
                     IGTFAQIVQPQLADVLNSSYELHCNEPSYASMYPTPWRYVNINYYAVVKPPTPETVFD
                     WKVWLVGFEYINGQPYLFGVTHFVWEP"
     gene            4828..5244
                     /locus_tag="PL000_12995"
     CDS             4828..5244
                     /locus_tag="PL000_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNC89864.1"
                     /translation="MELPIFLGSGLFTVAILLCTCLITLVSLGAMGFFFYRMFKNSSQ
                     NNAVLQSGIQAPAVILNVEDTGASMNDNPQVRFTLQVNPPDGRPSFQAVTTKFISRLQ
                     IGMINPGSPVTVRYDPNDTTKVAIETLGAPFVPPQK"
     gene            5317..5574
                     /locus_tag="PL000_13000"
     CDS             5317..5574
                     /locus_tag="PL000_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89865.1"
                     /translation="MAKLIPASERIIRAKKFIQEARELPIPAEGGRYNFSYVAGVRDL
                     LRQARDMVKFISMTPSATADMKAEVKKVIEEIERANTEILG"
     gene            5649..5906
                     /locus_tag="PL000_13005"
     CDS             5649..5906
                     /locus_tag="PL000_13005"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNC89866.1"
                     /translation="MMENKELEKLLEKLHAELEKVDHMDEDGMKLLRGIEKDINELLH
                     QTDRDTLIGRLREAIEKFEVSHPTLTTMLSEISSILNIAGI"
     gene            5916..6284
                     /locus_tag="PL000_13010"
     CDS             5916..6284
                     /locus_tag="PL000_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89867.1"
                     /translation="MEKKPSFLWALAIGAAFPILQVLVFLIRFNDLAVGASFADYIFF
                     FVGGLLIGLALIAALRRCETRGAYRATLIGFGLGLPFAVFGMVVGGMLGPVGVIMFSV
                     SPGVFFILVGYLIGRATFQV"
     gene            complement(6339..7187)
                     /locus_tag="PL000_13015"
     CDS             complement(6339..7187)
                     /locus_tag="PL000_13015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC89868.1"
                     /translation="MIDQITTHSGVLQSGQFVNYVQQGRGAPVILTHGLAASLHDWDD
                     LLPELAASGYAGFALDLLGHGESYKPVNHQDYSFDHVYDHYSAWVNSLNQTEPMILIG
                     HSLGGGLSLQYTLQHPERVRALVLVNPFYDIHQLPPVLRLAFRRKLINTSLIDRTPYR
                     LFRLMVDLTSFNYYIGHRETHVLPEHIRYQTALDYKRASSGIYNIPRSFPNLTSSLPQ
                     IKQPTLLLWGGRDQTLAPQSFPRIGEFLPNVVASHALPICGHVPHQCHPEKFNPHVMK
                     FLKDLA"
     gene            complement(7274..9745)
                     /locus_tag="PL000_13020"
     CDS             complement(7274..9745)
                     /locus_tag="PL000_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC89869.1"
                     /translation="MDIDFDLYRHEIRISSDPLVRLSAIDVSPELPKRTLVFIHGFGG
                     KAEQWQYQMQKFALDNRVIALDLRGHGLSDKPTSGYDMPRIQLDLETALTQLKVSTPF
                     VLIGHSFGGAVVTEYALNHPDHIEKLIIIATAGEFKLRPLFKFALNLPASILRLIGPF
                     TRKWLHAPPHALRPFYLENLSKWVGWERFAKLQVPTMIVRGHRDIVFDRPLFEKVAGS
                     IPGGEDVDIGVSGHMVMLERREAVDRAIARFLKGETKKSWRDDASLVVSKSTRDELKS
                     QRVWLNHYENGVPYTVDVPRIPAHHLLRSSVRRFPNHAAIFFEGAKLTYRKLNHEANR
                     FANALLAQGIGKGARVVLLLPNVPQMVIGFYGALKAGAAVVLIPPMIEPEELTRQIKE
                     ADASVLVTLSMWSGLAKQIQRDAGTPHVVLTDPAEYLPLPKYLISRWRNRGLDVQNAL
                     HWNRWLSAQSDKSPAVEVQPEDLAAIIFTGGTTAQSKGVMLSHRNLVANALQTRHWLP
                     EAMEGRERFLCVVPFFHSYGMTAALNVPVSLGAAMILKAQFQALEVLKTIKKYKPTIF
                     PGTPNMYVAITNFRGVRKYGIGSIKACISGSAPLHVEVQESFEKLTKGKLVEGFGLTE
                     ASPITHANPLGKNRKVGSIGVPLPSTHAAVVDLKSGRKEVEPGQIGELAVRGPQVMMG
                     YWKNEAATREVLTDDGWLLTGDVAQMDEEGYFRIVARKADMWYPEKPGAPAFPRDVEE
                     VIYEIPQVKETVVVAVAGHPFAFVIAGKEKPSAESVIAYCKRRLPPHLVPRFVIFMDD
                     FPRTFIGKVLRRELAKRYGKQIAGE"
     gene            10115..>12450
                     /locus_tag="PL000_13025"
     CDS             10115..>12450
                     /locus_tag="PL000_13025"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89870.1"
                     /translation="MGIYLVSRDLKKQFREREDILLSVRKSHELLLSSEEKFSKVFQL
                     SPAIIALTMLEDGTFLDINQKFLDISGFTRAEVIGKTSMEIGWVSPEVRDALIKEIQI
                     AGRVTNREVILHAKNGEEILCIYSGELLELGDKTYVLSLAQDITERRKAEEALQSSEA
                     QYRLITENMKDMVWLMDMEFKPIYISPSVTKLRGFTLNELQVLPLEKNLAPDSMRRAL
                     EVMNVSLAPEMIASTHSYKIELEYYKKDGSTYWSEDTFVLIRSEEGHPVGILGGGHEI
                     TEKKKAEDALRESEEILRTVFNTMEEGLALNELVYDENGEIVDYRILEVNPAYERITN
                     VPRDQAIGKLATEFYEMPTEYINAFWKENIDNDHIVTELYEDRLKAWKHISTSRPRDG
                     KFTTIFFDITEQKKSEEALRTSEQQYRLLFEEMMDGFALHEIICDENNIPIDYRFLAV
                     NPVFEKLTKLKAEDVIGKTVLEVMPDIEDVWIKRYGQVALTGEPAHFENFSTTLQTHF
                     EVRAFSPARGKFAVIFHDVTQQRNAQMIMLARMKFIESSTVQPLERLLQTILDEAEDM
                     TGSKVGFYHFVGADEQTLLLQSWSTRTASEFCKADGKGLHYPVERAGVWADCVREKRP
                     MIHNDYKSLPHRKGLPEGHAELIRELVVPVLRRGKVVALLGVGNKEYNYTEQDVAIIG
                     QLADLVWDIVESKRVEEIQKRVEQDYEALIQSIDGIVWEADVHTLEFSFVSQQAERLL
                     GYPIERWTTEPGFWIDHIHPDDRAASISFCVAQTRLKKS"
     assembly_gap    12451..12550
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12551..14724
                     /locus_tag="PL000_13030"
     CDS             <12551..14724
                     /locus_tag="PL000_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89871.1"
                     /translation="DRAASISFCVAQTRLKKSHEFEYRMFTADDRIIWLRDVVSVVLE
                     NDHPVRLRGIMIDITEKKMADDRLRQLSLVVEQSPASVVITDLNGNIEYVNQKFMQVS
                     GYTLDEVLYKNPNIVQSGKTPKETYAQLWKNITSGKVWHGEFQNKRKNGEYYWEDVLI
                     SPLLDTNGKITHFVSVKEDITERKQAENEIIEKSQELEALFKISSHLRTAQTVPEMIP
                     VVLKQMRETFNSDANAVILINRDHTQFKYVLGDGLLAENTGLQFGVEHSISRRVMDTL
                     EPYITLDFTSDLIGASAVKVIENIGATVVVPLQSETEFLGTLLCARAKDSQLAPFTSA
                     EIKLLRAIGEMVGNALRRAKLYDDALSRLQRVEALRSIDMAINANMDALLTLNMLLSQ
                     TIALLKVDAAAAMLYNPASHMLEYGAGQGFHHKEIEKANQKLNRGFANTAIIERRLVT
                     ARNLKLTEDQSYRAIAIKEGFETCHIVPMIANGQVCGILEVYNKTEISPDQEWLDFLE
                     ALGGQAAIAIHSAQLFENLEKSNLELSLAYDATIEGWSKALELRDKETEGHTLRVTEW
                     TVKLAMLAGMGEADIVQVRRGALLHDIGKMGIPDHILNKNGELTSQEWEIMRQHPQYA
                     YNMIHPISFLRPAIEIPYYHHEKWDGGGYPFGLKGEQIPYSARLFAIIDVWDAITSNR
                     PYRKAWSFEKALEYIKSESGRHFDPNIVELFLRMVNGFRNE"
     gene            14794..15123
                     /locus_tag="PL000_13035"
     CDS             14794..15123
                     /locus_tag="PL000_13035"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HNC89872.1"
                     /translation="MSLEKAVEAIIKEAQERGDFDNLKGKGKPIDLSAYFETPEELRN
                     AHAMLKSAGMVSAEVDLLQEIAALKERLAATYEEKDRSRIKKIIRDKELQFNVMLERQ
                     KRQRKRK"
     gene            15427..17070
                     /locus_tag="PL000_13040"
     CDS             15427..17070
                     /locus_tag="PL000_13040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNC89873.1"
                     /translation="MSKKNKLRIIPLGGLGEVGKNMMAYEFGGDIMVVDAGIMFPHND
                     MLGVDYIIPDFEYLMKRADRVRGLVITHGHEDHIGAIQHVIDQINVPIYATPLTRGLI
                     EGKLSRNNAQHKAQFKTIQAGESSNIGPFKVEYFHVSHSIPDAVGLGITTPAGLVVHM
                     SDFKFDHTPVDGWPTDFAKLSEFSTRGVDLLLSDSTNAERPGWTPSESVIGPAFDKVF
                     AEAKGRVIVATFASLISRVQQVADAAAKHGRKMALAGPSMVDNVKIARKMKYLNIPDE
                     MIVPIDQALNMQDHKVAIMCTGSQGEPSSIVGRLSAGTNRQFDLKPGDTIVLSSHPIP
                     GNEETISKTINRLLRRGARVIHDGIAPIHVSGHAAQEEQKLLINLVRPKHFMPIHGEL
                     RQLMRHAELAQQLGIEPENTIVVENGQVVELVGNKILLGERIPGDYVFVTGESIGDID
                     HDVMRERSQLARNGIMLIDISLDKLTGKLLHDPEIITRGFVSPEDAEILIPEVQKRVM
                     DVVHSGGLDDEKDIVNAVKSYLHQETKRRPMVFVTLSKA"
     gene            complement(17127..17993)
                     /locus_tag="PL000_13045"
     CDS             complement(17127..17993)
                     /locus_tag="PL000_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89874.1"
                     /translation="MSPSSKKSQTKKSFWDRLVQFIPVWVQIVICIGTIAVAAVPFVF
                     PRILSEETPTPTSALPTDVLPPAVSQAEEPTETPSPIPAPATPELVAPVLPPPVPGED
                     WARNCIDTTIWSTFLADKITRGSTPCYELTQWGITGESGKVIFVTHKSQSRAVEYGIF
                     TPWQNWKGVAFSVHKNHLENSEIWVGFFEEETVYSNGIVFVIQPDDVIDIRQLPVEDL
                     PVNNVGLPFADGVFNVDIVLREGKFKASVDGQGIISEWPLSFAIKYMFVGYRSLPSTN
                     LDASVSDLKFIK"
     gene            complement(18067..19101)
                     /gene="mutY"
                     /locus_tag="PL000_13050"
     CDS             complement(18067..19101)
                     /gene="mutY"
                     /locus_tag="PL000_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNC89875.1"
                     /translation="MPRLSSDLLNWYHKHGRILPWRDHPDPYAVWVSEIMLQQTRVET
                     VIPYFEKWMGLFPNIAALAKADEQTVLTAWEGLGYYSRARNLHKAAKIVVEKFGGELP
                     RNLDDLRSLPGIGRYTVGAIASMAFKMDEPTLDGNLRRVFSRLFDVTEPADAPAGEKI
                     LWELAAKHLPKGEAGDYNQALMDLGATICLPKTPRCLLCPLMQRCEARKNGTQELRPV
                     LKPKKQAPHHVHAAAVIIERGKVLLHQRPADGLLGNMWEFPNARIEADPASGLVTAID
                     AEYKLTVQRKDALGVVQHAYTHFKITVHAFRCKALSIPKNSNLKWVSLDALGDFPMGK
                     VDRQISKMLA"
     gene            complement(19136..21580)
                     /locus_tag="PL000_13055"
     CDS             complement(19136..21580)
                     /locus_tag="PL000_13055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC89876.1"
                     /translation="MKHTFTKYWPWFFFTAAFESLLAILALLWLPSESGLSMARLGLL
                     GVLFAFFCAGFYGGWIARQNPTRFEKLASTPAIIFTALLFLTSGLVLFLLRYLNPERL
                     LPYYERLSPLLWYLLIVSLQSSIFFLLHKNGFHLQDISARRPVYVSALIAFAFLFSVF
                     VFVAITKLGITKDTAYWGEPGVAIIGWQFMIALLAGFGLLVYNALNPSPSVGVTNYIL
                     PLTLYLTAAALWLSVPVDVLQNSFYSPITPPANTPFPYSDAGFYDYLAQSLLLGTGYL
                     NSIPPRPFYVFFLAILHFLFGQDYSAIITAQTLVLAFFPVSLYFLAKKLHTSAAGVTV
                     ALFAIFRELTGLWISSNTRVANSKIFTTDFPTAMALTVLCLVLIWWLERRDFKSTLIA
                     GGFFGLVLLFRTQSLLVLPVVFVLAWFAFQRKTKEWVMAGFVFAIAMILTVLPWLTHN
                     YTVIGQFTFDDPRQSAIIYSQYSFTGNLDLSQFDPAKESVGHRIVTFTLENPTYVAGF
                     ITNHILNTEIGGLLALPLIERFDGLLEPVNLYWVSWDGSLTWYNLVLLLVYLAVIAVG
                     FGVSWRRWQWIGLVPLAVNLGYVLANGISRFSSWRYNLPVDWVIYFYFAIGVVEILGG
                     LFLLFGVNPFSNLHAPSLSEQEPRGISFRDLRPQFAFFLLAFMFVGALPWFARGLAQP
                     RYAAPQSTLLETLITRGHDSTALTSFLSQPNAVLLEGQLLYPRMFRRGEGMASMNPWP
                     AYAIRDYSRIGSILLNDTRSDLIFVTKDLLNFQHGADAIVLACQTDEGYFNVRLIDFG
                     ETSFQSAPLMDPCPEN"
     gene            complement(21628..21930)
                     /locus_tag="PL000_13060"
     CDS             complement(21628..21930)
                     /locus_tag="PL000_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89877.1"
                     /translation="MSVKVIMTWDIAAEHDQEYFEFVIGEFVPGVQRLGLQPAEAWAT
                     IYGSYPQIQVGLLASDMNQARRILASPDWIALQERLFGYVKNYSHKVVPARGGFQF"
     gene            complement(21932..22576)
                     /locus_tag="PL000_13065"
     CDS             complement(21932..22576)
                     /locus_tag="PL000_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC89878.1"
                     /translation="MEITWYGHSCFRLTERNYATVVTDPYDSKKVGYEPLKLKAEIVT
                     LSHDAPGHNYSDAVKGSTHVLTGPGEFEIGSVFITAVQTDSAGKKKDKVRNTVYVFDY
                     DGITVAHLGDLQQIPTQSEIELLGTVNVALVPVGGGNSLNAAKAAEVVSMLEPNLVIP
                     MHYATPDSKVSLDAINKFIKEMGLSKPEAQPLLKVSRSGLPDETHVVVLDYQKG"
BASE COUNT         5889 a         5828 c         5673 g         5133 t
ORIGIN      
        1 tgaccaatga ataccttgtg gaaggtcttg atgactccga ttcatcggca ctctctgcct
       61 ccttcgatgc ggaagtggaa gaccccgcag gcgggtgcct gatattgaaa gatacccatg
      121 ttcgatccat gggaaacata cacacacctg cgcgcacctt tcccgaatgg catatcccct
      181 cgttgacaaa cgtcgtcgcc atgttctcag atgaccccgc agccgaagat attttgctcg
      241 aagtatggga agatcatcaa acaccccaca aggtcattat ttattcgggc gtgtatgtca
      301 ttgaaggcac catctactcg gaagaagaag ccccgcccga ttttatgctc cacgccttcg
      361 cgcccatcga aaacctgaag atcaccaatc aggtagataa aaaagcagaa cccatccacg
      421 ccaaatgggg cgtagtcaat tcgaccatga tgcagggcta tggagttgaa aaataaactg
      481 tctgtttttt aaccaagccc gcagaccgtg gaccaaggtc tgcgggtaaa tctttcactg
      541 ggggccaaca attcaaacca tccattttcc tgtcatgcta tttcaacact ccaacaagtt
      601 gattcgtgtc atcatgaaag atgaagactt gaacagacga tttgtcaggt ctgttcggcc
      661 atttagccgt gtgttcggct ggaggtcaag atgtttgcac gaaaactttt tgcaggggtg
      721 ctattggcgg gtcttgtttt ttcccaactt gcggtttcat cggcggcagc aaaagcctgt
      781 aatcaggctc aattcatctc cgacctgacc gtgcctgacg gcacgtccta taccgtggga
      841 accaccttca ccaaaacctg gcggttgctg aacattggct cctgcacatg gacaacctcc
      901 tacagtctgg tctttgtcaa aggtgaccaa ctgggtgcgc ctgcctcaac aaatctaccc
      961 gttgaggttg cccccggtca aatggtggat ctctccatta acttggccgc tccacaaaca
     1021 agcggtcact atcgtgggta ttgggccttg aacgacccca gcatcaccgc tggagcattt
     1081 ggaatcggca ccagcggcac agacccattc tgggtggata tcaacgtttt gggaaccagc
     1141 gccgtcattt atgattttgt tgccaacgca ccctacgccg aatggaaaag cggatcggga
     1201 atcctgccct acccaggtgc cagcggtgat aaccgtggat actcccaact gatcgatttt
     1261 ccccgccttg aaaacgattc gatcgattct cagcccgggc tgctcaccgt accccaaaac
     1321 aaatacaacg gattcattca agccacctat cctgaattcc tcgtcagcaa gggtgatcgc
     1381 ctgcaaacgc tggtcaattg cgaatatgga gccacaggct gttacgtcac ctttggcatt
     1441 gattacgtca cctccacggg cagtgtgaag ggattatgga gttggaagga agcccatgat
     1501 ggccgcttct atcgtgccga cattgacctg agtatccttg cggggaagaa ggtcaaattc
     1561 attctcaaac tgctttcggt aggatctgcc agtggtgacc gggcagtttg ggggtcgcca
     1621 cgaatcgtac gttccagcgt ggaaactcca ccggcgccgc catccacact gacaccactg
     1681 ccgccattga ccccgaccct gacaccattt gccacagcgc cgccgatctc tgcggcaggt
     1741 tgtaacaaag ccgccttcgt tgcagatatc accgtgcccg atggaaccct cttttcacca
     1801 ggctcagcct tcacaaaaac atggcgaatc aaaaacagcg gctcctgcgg gtggaccacc
     1861 gcctacagcc tgacgtatta cagcggcgag cggatgaacg cgccgacctc actgccccta
     1921 cccaaagcca tctctcccgg tgcgaccgtt gacctgactc tcaatatgac ggcacccgct
     1981 tcacctggaa aatatcgcgg gtactggatt ctgaaaaatg cggcggggga actattcgga
     2041 atcggcacca acgcttccat gccgatctgg atcgagatca atgtgacggg ttcgcccgcc
     2101 ctcaacacag gctacgattt tgccgccaat gcctgctcgg cagaatggaa gagcggtgcg
     2161 ggtatcctcc cctgcccggg cacaaccact gataaaaacg gcttcatcct caaaatggat
     2221 tcgtctcaac tcgaagatgg gaccacgaat tccaaccccg ctttgctggt cggcccgcaa
     2281 aacaaataca acggctatgt gcagggaatt taccccacca tgaccgtcca acccggggat
     2341 catttccgca cgacggtggg ttgtgaattt ggcaccagtt gttacgttgc cttcaaatta
     2401 gattacatga cctccacagg cacgatcacc aatttttgga catggcgcga acaaaacgaa
     2461 ggccgcccca acaacattga cctcgacctc acgccgctgg tcgggcgcag tgtgcgcttt
     2521 atcctcaccc tgtcggccaa cggctctgca accaatgacc gcgcactctg gtcggcacct
     2581 gtcattatcc gcacgaatct cacgcccaca tttacaccaa cctccagccc cacacctcaa
     2641 gtcaatgact ggttgaccta ctccaattca gatcatggat tccaattcaa atatccaaaa
     2701 tcgtcgcaga tcaatgaaat gttggagaat tcgatcagca tgaatttgcc cattgcggcg
     2761 ggcacaaacc tcagccaaaa atcactgcaa gtcattgcgg tgcaaaatgc aaatccgtgc
     2821 agcagttatc ttggcaggtc gtccatgttg agcagctctg aaactgtgct gatcaacggc
     2881 ctcaccttcc tcaaggaaac aggcggcgac agcgcggcag gacaactcca tcaatgggtg
     2941 gcctactcca ctttacgcgg tgacacctgc gtcaccttcg atatgatcct gcacatcatc
     3001 aacctgggta attacctcac accaccacct gcattcaacg aaacagccga aatttccgta
     3061 ttgaaggatg tggtctctac tttcacatgg ctgggagcca cccccacacc cactttcacc
     3121 ccaactcctt tcggctacga ttggcccgtt tatttcaatt caaaatacgg cttccagatc
     3181 caatacccaa aggaagcaac actggccatt caaaatgaca acgccgctca tctcaatctg
     3241 ccgtttgccg caggcaccaa cttgaaggaa aagattttcg atatcttcat tcgtgaagac
     3301 ctgcaaacct gtcgcagcat cattgcatcc tcgtccatgc tggtgacctc tgaaactgtc
     3361 acgatcaaca acaccacctt cttgaaagaa gtcggtgaag atggcgcagc aggcagttta
     3421 tatcaatggg ttgcctattc cacgctcaaa aataatgcct gcatcagttt cgaaatgagg
     3481 ctgcattcca tttctcctgg tgtgtacgaa acgccacccc cgatctttga ttatgccgca
     3541 gagacctcca tcattcaaaa tatgctcgcc acctttggct ggtctcctgc cacatccaca
     3601 ccaaccccaa ctgcaattcc cacatctgcc gccacactca gcgccatttt tgaagccatg
     3661 aacacgcgag attacggcac actgaaagcc tcaatgggtg aaacttttgc aatcgcatat
     3721 tggcaatcgc agggaacctc atccacgcca gatgaagcca tcgaacaact gaagacctat
     3781 tatcttgcct ctcccaaagc actctcctcc gatcccaata gagacctgac aaccctgctc
     3841 ggcggagcaa atccatacgc actcatgggg ctcgacccaa ccaagtcaca agcgctgctg
     3901 gtctcagggc tggggccgca gggtattgac gaagccattc tctacatgac ccaacgagcc
     3961 gatggcacgc cctactggtt tgggcttctc acagccaaag gcggtttcgt ctccttcacg
     4021 ccatatgcag tcacattgat cgcaacaggt ggagtgcttg atgtttattc cgctccgggc
     4081 atttccaacc cgatcgttgc ctccttcccc tttgatggga tcaacatcaa gcgcacgggt
     4141 cccactcaac tggaagctgg tactgaatgg gttgaagtga ttcgccccga cggagggacc
     4201 gggtgggtca attcccatta cctcacggaa tacatccccc gcgatggctt ctgcgcggat
     4261 gcccgcattg cgccgatcat cgcccagcta aagaactccg tcactctttc agatggtacc
     4321 ctgcttggtt cattgaccag ccccaaacat gggctgaacg taaattactg gcaatacagc
     4381 aacacggtca actacacttc ggctgccatt caaacgatct ataccgatcc acaggcaatt
     4441 gattggggca gcggcggcgc cagcggcatc accgacattg gcacattcgc acaaatcgtc
     4501 cagccgcaat tagccgatgt gctcaactca agctatgaat tgcactgcaa tgaaccatcg
     4561 tatgcctcga tgtacccaac cccgtggcgg tatgtcaaca tcaattatta tgcggtggta
     4621 aagccgccca cccctgaaac cgtcttcgat tggaaggtat ggctggtggg ctttgaatac
     4681 atcaacggcc agccgtatct atttggcgtg acccattttg tttgggaacc ataacatcat
     4741 gctcccccac ctttggggga gcatgtgtaa aatacacggt gatataatgc cgccaactca
     4801 accaacttga aataggagta tcaactcatg gaattgccca tttttcttgg ctctggcctg
     4861 ttcaccgttg cgatcctttt atgcacctgc ttgatcaccc tggtttcatt gggagcgatg
     4921 ggctttttct tctaccgcat gttcaagaat tcaagccaga acaatgccgt ccttcaatcg
     4981 ggtattcagg ctcctgctgt aattctcaat gtggaagata ccggcgcctc catgaatgac
     5041 aatccacagg ttcgtttcac actgcaggtc aaccctccgg atgggcgtcc ttcctttcag
     5101 gcggtgacca ctaaattcat aagccgcctg cagatcggca tgatcaaccc tggctcccct
     5161 gtcacagtcc gttacgaccc gaacgatacg accaaggtcg ccattgaaac actgggtgcg
     5221 cctttcgtcc ctccgcaaaa gtagacttca tcatcccagc ccaccccgga cgcggcaatc
     5281 gctgcgtcct ttttattaca aagcaaagga gcaaacatgg caaaactcat tccagcaagc
     5341 gagcgcatca tccgcgccaa aaaattcatt caagaggcgc gcgaactccc catcccagcc
     5401 gaaggcggac gatacaattt ctcttacgtg gcgggcgtgc gagacctgct gcgtcaagcc
     5461 cgtgacatgg tcaaattcat ttccatgacc ccctccgcca ccgcggatat gaaagccgag
     5521 gtaaagaagg tcattgaaga aatcgaacgc gccaacacag agatattggg atgacatatt
     5581 cacccggaac gttgtaaaat tgaattagag gcaggtgcgg gttcacaaat ccaaatgacg
     5641 agaggtgcat gatggaaaac aaagaacttg agaaactgct tgaaaagctc catgccgaac
     5701 tggaaaaagt ggatcatatg gatgaagacg gtatgaagct cctgcgtggg atcgaaaagg
     5761 acatcaacga actactccat caaaccgacc gcgataccct gattgggcgt ctgcgggagg
     5821 ccattgagaa atttgaagtg tcgcatccca cactcaccac catgctttct gaaatctcct
     5881 ccattttgaa cattgctggc atctagggaa taattatgga aaaaaagcca tcttttctat
     5941 gggcactcgc aattggggcg gctttcccca ttttgcaggt gttggtgttt ctcatccgtt
     6001 tcaatgacct tgccgtcggg gcatccttcg cagattatat tttcttcttc gtcggcggcc
     6061 tgttgatcgg gctggcattg atcgccgctc tgcgccgttg tgaaacacgg ggtgcatacc
     6121 gcgccacgct catcgggttt ggcttggggc tgcccttcgc cgtattcggt atggtggtgg
     6181 gaggcatgct cgggccagtg ggtgtgatca tgttcagtgt atcccctggc gtgttcttta
     6241 ttctggtcgg ctatttaatt gggcgggcta cgttccaggt gtagcaggtt gataaaattt
     6301 gattttgaaa aatcccgctc cgatgagcgg gattttttct atgcgaggtc tttcaaaaat
     6361 ttcatcacat gcggattgaa tttttcaggg tggcattgat gcggcacatg cccgcaaatg
     6421 ggcagggcgt gtgaagcaac cacattgggg agaaactcac caatgcgcgg aaaagattgg
     6481 ggtgccaggg tttggtcacg tccaccccac aggaggagag tcggctgttt gatctgtggc
     6541 aggcttgagg tcaggtttgg aaacgagcgc ggaatgttgt agattccaga agaggcgcgt
     6601 ttgtaatcca gcgcggtttg atatcgaata tgttcaggca ggacgtgtgt ttcgcgatgg
     6661 ccgatgtagt agttgaaact ggtcaaatcc accatgagac ggaaaaggcg atagggggtg
     6721 cggtcgatca agctggtgtt gataagtttg cgtcgaaacg caagccgcaa aacaggcggc
     6781 aactgatgaa tatcataaaa cggattgacc agcaccaatg cacggacgcg ttcaggatgt
     6841 tggagcgtgt attgcaaaga gaggccgccg cccaacgaat gcccgatcaa gatcatgggc
     6901 tcggtctggt tgagcgagtt gacccaggcg gaataatggt cataaacatg atcaaacgag
     6961 taatcttgat gattgactgg cttgtaactt tcgccgtgcc ccagcaggtc aagtgcgaag
     7021 cccgcgtaac cggaagcggc aagttcgggc aaaaggtcat cccaatcatg taaggatgca
     7081 gcaagcccat gtgtgagaat gacaggtgcc cccctgccct gttgcacata atttacaaac
     7141 tgcccgcttt gcagcacccc actgtgtgtt gttatctgat cgatcatttc cattttctca
     7201 aaaatccttt ttacaagaca agggcgaagt ttttcatcct tcatccttta tctttcatcc
     7261 ttgttgtttt accctattct cctgcaattt gttttccata gcgtttggcc agctcacgcc
     7321 gtaatacctt gccaatgaag gtgcgtggaa aatcatccat gaagatcacg aagcgtggaa
     7381 caaggtgcgg gggcaggcgg cgtttgcaat atgcaatgac cgattctgca cttggtttct
     7441 ctttgccagc aatgacaaag gcaaaaggat gccccgccac cgcaacgacc acggtttcct
     7501 tgacctgcgg aatttcgtag atcacttcct ccacatcgcg cgggaaggca ggcgcaccag
     7561 gtttttcggg ataccacatg tcagccttgc gcgcgacgat gcggaaatat ccttcttcat
     7621 ccatttgagc cacatcgccg gtaagcagcc agccatcgtc ggtgaggact tcccgtgtgg
     7681 cggcttcgtt cttccagtac cccatcatca cctgcggacc acgcacagcc agttcaccga
     7741 tctgcccagg ctccacttct ttgcggcccg atttcagatc cacaactgcg gcgtgtgtgg
     7801 atggcagcgg aacgccaatg ctgcccactt tgcggttctt tccaagcggg ttggcgtggg
     7861 tgatcggcga agcctcggtc aggccaaaac cttccacaag ttttcctttg gtgagctttt
     7921 caaaagattc ttgcacttcc acatgcaaag gcgcagaacc gctgatgcag gctttgatgg
     7981 aaccaatgcc atatttcctc acaccgcgga aattggtgat cgccacatac atatttggcg
     8041 taccggggaa gatggtcggc ttgtattttt tgatggtctt caaaacttcc agagcctgaa
     8101 attgtgcctt caagatcatg gccgcaccaa gtgaaacagg gacattcaat gcagcggtca
     8161 tgccataact atggaagaaa ggcacaacac ataagaaccg ctcacggcct tccatggctt
     8221 cgggcagcca gtgacgggtt tggagtgcat tcgccacaag gttacgatga gaaagcatga
     8281 cacccttgga ttgtgccgtg gtgccgccag tgaaaatgat ggctgcaagg tcttcaggct
     8341 gcacttccac agcgggagat ttatcgcttt gtgcggagag ccagcgattc caatgcaggg
     8401 cgttttgcac atccaacccg cggttgcgcc agcgtgagat gagatatttt ggaagcggta
     8461 gatattctgc ggggtcggtc agcaccacat gcggcgtccc tgcatcccgt tgaatttgtt
     8521 tcgccagacc cgaccacatt gaaagagtaa ccagcacact ggcatcggct tctttgatct
     8581 ggcgtgtgag ttcctcgggt tcgatcatcg gcggaatcaa cacaacagcc gcacccgcct
     8641 ttaacgcgcc gtaaaatccg atcaccattt gcggcacatt cggcaaaagc aaaaccacgc
     8701 gcgcgccttt accgataccc tgtgcaagca gggcatttgc aaagcgattc gcttcatggt
     8761 tcaattttcg ataggtcagt tttgcccctt caaaaaagat ggcggcatgg ttcgggaagc
     8821 gccgcaccga agaacgcagc aaatgatgcg caggaatgcg cggtacatcc accgtgtagg
     8881 gcacgccatt ttcataatgg ttcagccaaa cacgctggct cttcaattcg tcacgggtgg
     8941 atttggaaac gaccagggag gcatcgtcac gccaggattt tttcgtttcc cctttgagaa
     9001 aacgggcgat ggcacgatcg accgcttcgc ggcgttccaa catgaccatg tggcctgata
     9061 cgccaatatc aacatcttct cccccgggaa tggatcctgc caccttctcg aacagtggtc
     9121 ggtcgaagac aatgtcacga tgtccgcgca cgatcatggt aggcacttgc agttttgcga
     9181 agcgttccca tcccacccat ttggaaaggt tctcaagata gaacgggcgc aaggcatgcg
     9241 gaggggcatg cagccatttt cgcgtgaatg gtccgatcag gcgcaaaatg gaagcaggca
     9301 ggttcaacgc aaatttgaac aggggcctga gtttgaattc gccagcggtg gcgatgatga
     9361 tcaatttttc aatatggtcg gggtgattga gggcatattc agtgacaacc gcaccgccga
     9421 aggaatggcc gatcaacaca aaaggtgtgg acactttcaa ttgtgtcaga gcagtttcca
     9481 ggtccagttg gatgcggggc atgtcgtagc cagaggttgg cttgtcggag aggccatgtc
     9541 cgcgcaggtc aagtgcaatc acgcggttat ccaatgcgaa cttttgcatt tgatactgcc
     9601 attgctctgc cttgccgcca aaaccgtgaa taaaaacgag agtccgtttt ggaagttcgg
     9661 gtgaaacgtc gatcgccgag aggcgcacca gaggatctga tgaaattcga atttcgtggc
     9721 ggtacaggtc gaagtctatg tccattggag aagtttacat gaaggggtga cgaatgtcaa
     9781 tttacggaga ggttcgataa tgctatacta aaaaatgcgc ttttttttca ggcatccaca
     9841 aaaactccag ccgacttata aataattgag gcctgccatg gttgattcca ctgattcttt
     9901 ttttcgaggg cgctcttaca caataatatc cttaattgga tggaccgcag ttactctggg
     9961 actcatctgg ttccatcttg gatggttgaa tgacctgcac ctgtcaccag atgaatcaaa
    10021 caagccctgg ggggattcgt tttaccatac ctggggccag gagtatctgt ccatattatt
    10081 aagtggatat ggcacgcttt ggctggtggg ctccatgggg atctatcttg tatcgcgtga
    10141 tctcaaaaag caatttcgag aaagggaaga tatccttctt tcggttcgaa aaagccacga
    10201 attgcttttg agcagtgaag aaaaattttc gaaggtattc caactttccc ccgccattat
    10261 cgcattgacc atgctggaag acggcacatt tttggatatc aatcagaaat tccttgatat
    10321 ctcaggcttc acccgcgctg aagttatcgg aaagacttca atggaaattg gctgggtctc
    10381 ccctgaagtt cgtgatgccc ttataaaaga aatccaaatc gcgggacgag tgaccaacag
    10441 agaggtcatt ctacatgcaa aaaatgggga ggaaattctt tgcatttaca gtggcgaatt
    10501 gctcgaactg ggagataaaa cctatgtgct ttctctggca caggatataa ccgaaaggcg
    10561 aaaagcggag gaggcactgc aaagcagcga agcccagtac cgcctgatca ccgaaaacat
    10621 gaaggacatg gtctggttga tggatatgga gttcaagcca atatacatca gcccgtcggt
    10681 cacaaaactg cgtggtttca ccttgaacga attacaggtg cttccattgg aaaaaaacct
    10741 ggcccctgat tccatgcggc gggcacttga ggtcatgaat gtgtccctcg cccctgagat
    10801 gatcgccagc acccattcct ataagatcga actcgaatat tataaaaaag atggaagcac
    10861 ttattggagc gaagatacat ttgttttgat acgaagcgaa gagggacacc cggtcggcat
    10921 tttgggcggc gggcatgaga tcactgaaaa gaaaaaagcc gaggatgcct tacgcgaaag
    10981 cgaagaaatc ctgcgcacgg tgttcaacac catggaagaa ggcctcgctc taaacgagtt
    11041 ggtctacgat gaaaacggag aaatcgtgga ctatcgtatt ctggaggtca acccggctta
    11101 cgagcgcatc accaatgtgc cgcgtgatca ggcaatcggc aaactggcaa ccgagtttta
    11161 tgaaatgcct acggaatata tcaatgcctt ctggaaagag aacatcgata acgaccatat
    11221 tgttacagag ctgtacgaag accgtttgaa ggcatggaag cacatttcca cttcgcggcc
    11281 cagagacggt aaattcacca ctattttctt cgatatcacc gagcagaaaa aatcggagga
    11341 agctttgcgc accagtgagc agcaatatcg cctgctcttc gaagaaatga tggatggttt
    11401 tgccctgcac gagatcattt gcgatgaaaa taacataccg attgattatc gtttccttgc
    11461 ggtcaacccg gtattcgaaa aattgaccaa attaaaagca gaagacgtta taggcaaaac
    11521 cgttctggaa gtcatgccag atattgagga tgtctggata aaaagatacg gccaggtggc
    11581 tctgacaggt gaacctgccc attttgaaaa cttctctaca accctgcaaa cccatttcga
    11641 agtaagggcc ttttcgcctg cgcgcggaaa atttgcagtg atcttccatg atgtgaccca
    11701 gcaaaggaat gcccagatga tcatgctggc tcgcatgaaa ttcattgaga gctccaccgt
    11761 acaaccgctt gaacgtttac tgcagaccat tctcgatgag gccgaagata tgacgggcag
    11821 caaggtcggg ttctatcatt ttgtgggtgc agatgagcaa accctgttgt tacaaagttg
    11881 gtccacacgt acagcatctg agttttgcaa ggcagatggc aaaggcctgc attatccagt
    11941 ggaaagagcg ggggtgtggg ctgattgtgt ccgtgaaaaa aggcctatga ttcacaatga
    12001 ttacaagtct ctgcctcacc gcaaaggact ccctgaaggt cacgctgagc tcatacgcga
    12061 attggttgtc cctgtattgc ggaggggaaa ggttgtggca cttcttgggg tcggaaacaa
    12121 ggaatacaac tataccgaac aggatgttgc catcatcggt caacttgcag atctggtgtg
    12181 ggacattgtg gaaagcaagc gggttgaaga gatccaaaaa agggtcgaac aggattacga
    12241 ggctctcatc caaagcattg acgggatcgt ctgggaagcc gatgttcata cgctcgaatt
    12301 ctcctttgtc agccagcagg cagagcgttt gctgggctat cccatcgaac gctggaccac
    12361 cgaaccagga ttctggatcg accacattca ccccgatgac cgtgccgcct cgatcagctt
    12421 ctgtgtggca cagacccgcc tgaaaaaatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn atgaccgtgc cgcctcgatc agcttctgtg tggcacagac ccgcctgaaa
    12601 aaatcgcatg aattcgaata ccgcatgttc actgcagatg acaggatcat ttggctgcgc
    12661 gatgtggttt ctgtggtgct tgaaaacgac caccccgtaa gattacgcgg catcatgatc
    12721 gatatcaccg agaaaaaaat ggcagatgat agactgcgtc agctttcatt ggttgtagaa
    12781 caaagccccg cttcagtggt catcaccgac ctcaatggca atattgagta tgtcaaccaa
    12841 aaatttatgc aggtcagcgg ctacacactt gacgaagtcc tctacaaaaa ccccaatatc
    12901 gttcaatcag gaaagacccc caaggaaacc tatgcccaac tgtggaaaaa catcacctca
    12961 ggcaaggttt ggcatggcga atttcagaac aaacgcaaaa acggcgaata ctattgggaa
    13021 gatgtgctca tttcaccgct gctcgacacc aacggaaaga tcacccactt tgtcagcgtg
    13081 aaggaagata tcaccgaacg caaacaggcg gagaatgaga tcatcgaaaa atcgcaggaa
    13141 ctggaagccc tgttcaagat ttcatctcat ttgcgcacgg cccaaaccgt acccgaaatg
    13201 atccccgtag tcctcaaaca aatgcgggaa acatttaact cagatgcgaa cgctgtcatt
    13261 cttatcaaca gggaccacac tcaattcaaa tatgttctcg gtgatggcct gttggctgaa
    13321 aacacagggc tgcaatttgg cgttgagcac agcatcagcc gccgtgtaat ggatacactc
    13381 gaaccctaca tcacccttga ttttacaagc gacctgatcg gcgcatccgc ggtgaaggtc
    13441 attgagaata ttggcgcaac ggtcgtcgtc cccttgcaat cagaaacgga attcctcggc
    13501 actttattat gtgcgcgggc aaaagactct cagcttgcgc cattcacctc tgctgaaatc
    13561 aaactactgc gggccatcgg cgaaatggtt ggaaatgcgc taaggcgggc aaaactctat
    13621 gacgatgccc tctcccgtct gcaacgcgtg gaagccttgc gctccataga catggcgatc
    13681 aatgcaaata tggatgcatt gctcaccctg aacatgctcc tcagccagac catcgccctc
    13741 ttaaaagtgg atgccgccgc cgccatgctt tacaacccag cctcccatat gctcgaatac
    13801 ggtgcaggac agggttttca tcacaaagaa atcgaaaaag cgaatcaaaa attaaatcgc
    13861 ggctttgcaa atactgccat tattgagcgg cggcttgtaa cagcccgcaa cctcaaactg
    13921 accgaagacc aaagttaccg tgcgattgcg atcaaggaag gttttgaaac ctgtcacatc
    13981 gtcccgatga tcgccaacgg gcaggtgtgc ggcatccttg aggtttacaa caaaacggaa
    14041 atctccccag accaggaatg gctcgatttt ctcgaagccc ttggcggaca ggcagccatt
    14101 gccattcaca gcgcacagct tttcgagaac cttgaaaaat caaatctcga actctccctc
    14161 gcctacgacg ccaccatcga gggttggtcg aaagcgctgg aactgagaga caaggaaacc
    14221 gaaggacaca cactgcgcgt cacagaatgg acggttaaac ttgcaatgct ggcaggtatg
    14281 ggcgaggccg atatcgttca agtgcgccgc ggcgccctgt tgcacgatat cggtaagatg
    14341 ggcatccctg accatatcct aaacaagaac ggcgaactaa cctctcagga atgggagatc
    14401 atgcgtcagc acccgcaata tgcatacaac atgatccacc ccatttcatt cctgcgcccc
    14461 gccattgaaa tcccgtatta tcaccatgaa aaatgggacg gcgggggata tccatttgga
    14521 ttgaaagggg agcaaattcc ctactcggcc cgtctattcg ccatcattga tgtctgggat
    14581 gccattacct cgaaccgccc ctatcgcaag gcgtggtctt tcgaaaaagc attggaatat
    14641 ataaaatcgg aatcgggcag gcacttcgac ccaaatatcg tcgaactctt tttgaggatg
    14701 gtcaacggat ttaggaatga atgacccaaa gcccgcgctt cgccgctgac ctcgcaactg
    14761 gtcgggcgaa attgacattc aaggagtctg gagatgagtc tcgaaaaagc agtggaagcc
    14821 atcatcaaag aagcccagga acgcggcgat tttgacaacc tgaaaggcaa gggaaaaccc
    14881 attgaccttt cagcttattt tgaaacgcca gaggaactcc gcaacgcgca tgccatgttg
    14941 aaaagtgcgg gcatggtttc tgccgaagtg gacctcttgc aagagatcgc cgccctgaaa
    15001 gaaagactcg cagccaccta cgaggaaaaa gaccgcagcc gcatcaagaa aataatccgc
    15061 gacaaggaat tgcagttcaa tgtaatgctc gaacggcaaa aacgtcagcg caagagaaaa
    15121 taacagaaaa cagaaacagg cgtcggcggg gagggatgcg gcccccgttc agcagaaaaa
    15181 agccgctttg acaaatcatc cccgccctgc tatactctta acagttaagt tgactttctc
    15241 tgccattcag gcagtttgtt tgagaacaat cgtgttttga aataccgatc cttcgcgagc
    15301 aagtcagcgg aggtcttttt tttgtgggaa aagactccga ggaacggacc agaaatatat
    15361 caaccctgac ttttaccgca taagcggttt aacatatcaa ctctttgtga ggtttataaa
    15421 aaatatatga gtaaaaagaa caaattaaga attatcccgc tcggggggct gggcgaagtg
    15481 ggaaagaaca tgatggccta cgaattcggc ggcgacatca tggtggtgga tgcaggaatc
    15541 atgttcccgc ataacgacat gctcggggtg gattacatca tccccgactt tgagtattta
    15601 atgaaacgcg ccgaccgtgt gcgcgggctg gtcatcaccc atggtcacga agatcatatc
    15661 ggcgcgatcc agcatgtgat cgaccagatc aatgtgccca tctacgccac cccgctgacc
    15721 cgcgggctga tcgaggggaa attatcgcgc aacaacgccc agcacaaggc ccagtttaaa
    15781 accattcagg ctggcgaatc ttccaatatt ggccccttca aggtggaata ttttcatgtc
    15841 agccattcca ttccagatgc ggtgggattg ggcatcacca cacccgctgg tttggtcgtt
    15901 catatgagcg atttcaaatt tgaccatacc cccgtggatg gctggccgac cgattttgca
    15961 aaactctccg agttttccac ccgcggtgtg gacttgctgc tttctgattc aaccaatgcc
    16021 gaacgccccg gttggactcc atcagagtct gtgatcggcc ccgcattcga taaggtcttt
    16081 gcagaagcaa aaggccgtgt gatcgtggcg acctttgctt cgttgatttc acgcgttcag
    16141 caggtggctg atgccgctgc gaaacatggg cgcaaaatgg cccttgctgg ccccagcatg
    16201 gtggataatg taaagatcgc ccgcaagatg aagtatttga acatccccga tgagatgatc
    16261 gtacccattg accaggcttt gaacatgcag gaccacaagg ttgcgatcat gtgtactggc
    16321 tcacagggcg aaccctcttc catcgtcggg cgtttatcgg ctgggacaaa tcgtcaattt
    16381 gacctgaaac ccggtgacac cattgtgctt tcgtcgcacc cgattccggg caacgaagaa
    16441 accatcagca aaacgatcaa ccgcctgttg cgccgaggcg cacgggtgat ccatgatggg
    16501 attgccccca tccatgtttc gggtcatgcg gcgcaggaag agcagaaact gctcatcaac
    16561 cttgtgaggc cgaagcattt catgccgatc cacggcgagt tgcgtcagct catgcgccac
    16621 gccgaattgg cgcaacaact tggaattgaa cctgaaaaca ccatcgttgt cgaaaatgga
    16681 caggtggtgg aactggtcgg taacaagata ttgcttggcg aacgcatccc aggtgattat
    16741 gtatttgtca ccggtgaatc cattggcgat attgaccacg acgtgatgcg cgaacgcagc
    16801 caattggccc gcaacggcat catgctgatc gacatcagtc tcgataaact cacgggcaaa
    16861 ttgcttcatg accccgaaat catcacccgc ggatttgtct ctccagaaga tgctgaaatc
    16921 ctgattcctg aggtgcaaaa acgggtgatg gatgtcgtcc acagcggcgg gttggacgat
    16981 gaaaaagaca ttgtcaatgc tgtgaagagc tacctgcatc aggaaaccaa acgcaggccc
    17041 atggtgtttg tgaccttgag caaggcgtaa aatttgaaaa gtagaaagat gacaccccgt
    17101 cgattggcgg ggtgtttttt tgaaggctat tttataaatt tcaggtctga aacgctggca
    17161 tccaaatttg tacttggcag ggaacgatat ccgacaaaca tgtatttaat cgcaaaactc
    17221 aacggccact cagagataat accctgacca tccacagacg ccttgaattt tccttccctc
    17281 aaaacaatat ccacattgaa cacaccgtcg gcaaatggca aaccgacatt attcacaggc
    17341 aggtcttcca caggcaattg ccgaatatcg atcacatcat caggttgaat cacaaatacg
    17401 atcccgttcg agtacacggt ttcttcttcg aaaaagccaa cccaaatttc gctgttttcg
    17461 aggtggtttt tatgcactga aaacgccacc cctttccagt tctgccacgg ggtgaatatc
    17521 ccatattcca ccgcacgcga ttgagattta tgggtaacaa agatcacctt gccgctttca
    17581 cctgtaattc cccattgtgt cagttcgtag cagggtgtcg atcccctcgt gatcttatct
    17641 gctaaaaacg tcgaccaaat tgtggtgtca atacaatttc gcgcccaatc ttcaccaggc
    17701 acgggaggcg gcagcacagg agccaccaac tctggggttg caggagcagg aatcggcgaa
    17761 ggcgtttccg ttggttcttc cgcctgactt acggcagggg gtaaaacatc cgtcggtagc
    17821 gcggaagttg gggtgggggt ttcttcggaa agtatgcggg ggaaaacaaa aggaacagcg
    17881 gctaccgcaa ttgttccaat acaaataacg atctgcaccc aaacagggat aaattgaaca
    17941 aggcgatccc aaaagctttt tttagtttga ctctttttac ttgacggact catccatgcc
    18001 tcccaaacac cgcagtattg ttttcttcgt agtttcagta taacgaaaat tttctcaagt
    18061 gcaatatcac gccagcattt tcgaaatttg cctatccacc ttgcccatgg ggaaatcacc
    18121 caacgcatcc aggctgaccc acttcagatt tgaatttttt ggaattgaaa gcgccttaca
    18181 tcgaaacgca tggaccgtaa ttttgaaatg ggtataagcg tgttggacaa cccccaacgc
    18241 gtcttttctc tggaccgtga gcttgtattc ggcatcaatg gctgtgacca accctgaagc
    18301 agggtcagct tcgatgcgtg catttggaaa ttcccacata ttgccgagca acccatctgc
    18361 ggggcgctgg tgcaacaaca ccttgccgcg ttcgatgatc accgccgcag cgtggacgtg
    18421 atgcggggcc tgtttcttcg gtttcagcac agggcgtaat tcctgtgtgc cgttttttcg
    18481 ggcttcacat cgttgcatca gcgggcacaa caagcagcgc ggggttttcg gcaggcagat
    18541 ggttgccccc aaatccatga gagcctggtt gtagtcgcct gcttctcctt ttggcaaatg
    18601 tttggcagcc aactcccaca atattttttc gccagcaggt gcatcggcgg gctctgtcac
    18661 atcaaacaag cgcgaaaaca cacgccgcag gttcccatcc agagtgggtt catccatttt
    18721 gaaagccatg gatgcgattg cgccgacggt ataacgccca atccctggca ggctgcgcag
    18781 gtcatccaaa ttacggggta attcgccgcc aaatttttca acgacgatct tcgcggcttt
    18841 gtgcaggttt ctggctcggc tgtaataccc caacccttcc catgcagtga ggacggtttg
    18901 ttcatccgct tttgccagtg cggcaatgtt ggggaataat cccatccatt tttcaaaata
    18961 gggaatcacc gtctcaacac gggtttgttg cagcatgatc tccgaaaccc aaaccgcgta
    19021 tggatcaggg tggtcgcgcc aggggagaat ccgcccgtgt ttgtggtacc agtttagaag
    19081 gtcagaagag aggcggggca tgttgggaag aatgtgttgc ttcggtgagc agtcgtcagt
    19141 tttcggggca ggggtccatt aacggggcag attgaaatga agtttcgcca aaatcaatca
    19201 agcggacatt gaaatagcct tcatccgtct ggcaggcaag gacaattgca tccgcaccgt
    19261 gttgaaaatt gagcaggtct tttgtgacga atatcagatc actgcgcgtg tcgttgagca
    19321 ggatcgagcc gatgcgcgaa taatcgcgaa ttgcatacgc aggccaggga ttcatagagg
    19381 ccatcccctc ccctcttcgg aacatgcgcg ggtagagcag ttgcccctcc aacaatacgg
    19441 cattgggctg ggaaaggaaa ctggtcaggg cagtgctgtc atgtccgcgt gtgatgaggg
    19501 tttcaagcag tgtgctttgc ggggcagcgt aacggggttg ggccaatccc ctggcaaacc
    19561 aggggagcgc gccaacaaac ataaaagcca gaagaaaaaa tgcaaattgc gggcgtaggt
    19621 cacggaagga aataccccgc ggttcttgct ctgaaagaga tggggcatga agattcgaaa
    19681 aaggattcac gccaaacaga agaaaaagac cgcccaaaat ttccaccaca ccaatggcga
    19741 aataaaaata aataacccag tccacgggca ggttgtagcg ccagcttgaa aatcgtgaga
    19801 taccatttgc aagcacatat cccaaattga ccgccaacgg cacaagcccg atccattgcc
    19861 agcggcgcca ggaaacgcca aaccccacgg caatgacggc cagataaacc aaaagcagga
    19921 caaggttgta ccaggtgagg gagccatccc atgaaaccca atacaggttg acaggttcca
    19981 acaacccgtc aaatctttcg atgagtggaa gcgcaagcag gccgccgatc tcagtattaa
    20041 ggatgtgatt ggtgatgaag cccgctacat aggttgggtt ttccaatgta aaggtgacaa
    20101 tacggtggcc gacactttct tttgcagggt cgaactggct gagatcaagg ttgccagtga
    20161 aggagtattg gctgtaaatg atggcggatt gacggggatc atcaaaagta aactgaccga
    20221 tcacggtgta gttgtgcgtc agccacggca ggacggtcag aatcatggca atggcaaaga
    20281 caaacccagc catcacccac tccttggttt tgcgctgaaa ggcaaaccac gccagcacaa
    20341 aaacgacagg caagaccagt aaagactggg tgcggaacaa aagcaccaaa ccaaagaatc
    20401 cgcctgcgat cagcgtggat ttgaaatcac gccgctccaa ccaccagatg agaacaaggc
    20461 atagtacggt caatgccatg gcagtgggga aatcggtggt gaatattttc gaatttgcca
    20521 cacgggtgtt cgatgagatc cacagcccgg tcagctctcg gaagatggcg aagagggcca
    20581 ccgtaacacc tgcagcggag gtgtgtagtt tctttgcaag gaagtacagg ctgacaggaa
    20641 aaaatgccaa taccagggtc tgtgcagtga tgatggcaga ataatcctgg ccgaacagga
    20701 aatgcaggat cgccaggaag aaaacataaa acgggcgcgg tggaatgctg ttgaggtagc
    20761 ctgttcccag caagaggctt tgggcaaggt aatcataaaa acccgcatcg gaatacggga
    20821 agggcgtatt ggcaggcggg gtaatgggtg aataaaaact gttctgtaaa acatctacgg
    20881 ggacactgag ccagagtgcg gcggcagtga ggtaaagagt caatgggagg atatagtttg
    20941 tgacacccac ggatggagat ggattaagag cgttgtatac aaggagaccg aaccctgcaa
    21001 gcagggcgat cataaattgc cagccaatta tggctacacc gggttcaccc caataggcgg
    21061 tgtctttggt gatgccaagt ttggtaatgg ctacaaatac gaatacagaa aacaggaacg
    21121 caaaagcgat aagagcagaa acgtaaacag gtctgcgagc cgaaatatcc tgtagatgaa
    21181 atccattttt gtggaggaga aagaaaatgc tgctttgcag ggaaacgatc agcagatacc
    21241 acaacagcgg actgagacgt tcgtaatacg ggaggagtct ttcagggttg agatagcgca
    21301 gtagaaacag gaccaagccc gaggttagaa aaagaagcgc ggtgaaaatg attgcggggg
    21361 tgctggcaag tttttcgaat cgggttgggt tctggcgggc gatccatccc ccataaaatc
    21421 cagcgcaaaa aaatgcaaaa agaactccga gcaagccaag gcgggccatg gacaagccgc
    21481 tttcggacgg cagccataaa agtgccagaa ttgccagcag ggactcaaag gccgcggtga
    21541 agaaaaacca cggccaatat ttcgtaaagg tatgcttcat tcgagaaagg aaaaaaatgc
    21601 gggtcggggt ttagccgacc cgctcgatta aaattgaaag ccgccgcggg caggtacgac
    21661 cttgtgtgaa taatttttca cgtatccgaa aagccgttct tgcagggcga tccaatcagg
    21721 tgaggcaagg atgcggcgcg cctgattcat atctgaagca agcaaaccca cctgaatttg
    21781 tggataggaa ccataaatgg ttgcccatgc ctcggcaggt tgaagcccaa gccgttgcac
    21841 accgggcaca aattcgccga tgacgaattc aaaatactcc tgatcgtgtt cggcggcgat
    21901 atcccaggtc atgataactt tgaccgacat attatccttt ttggtagtct aaaactacaa
    21961 catgggtttc atcagggagg ccagagcggg agaccttgag caggggttgc gcttcaggtt
    22021 tgctcagacc catctccttg atgaatttat tgatcgcatc caggctcacc tttgaatcgg
    22081 gggtggcata atgcatgggg atgaccagat tcggttcaag catgctgaca acttccgcgg
    22141 ctttggcggc gttgaggctg tttccgcctc cgacgggtac cagtgccacg ttgacagtgc
    22201 cgagcagttc gatctcgctt tgtgtgggga tctgctgcag gtcacccaga tgagcaacgg
    22261 taataccatc atagtcaaac acgtaaaccg tgttgcgaac cttatccttc tttttgcctg
    22321 cgctgtcggt ctgaacggcg gtgatgaaaa cactgccgat ttcgaactcg ccgggaccgg
    22381 tcagcacatg ggtggagcct ttgaccgcgt cgctgtagtt gtgcccgggc gcgtcatggc
    22441 tgagtgttac gatctcggct ttaagtttta acggttcgta gccaaccttt ttgctatcgt
    22501 atggatcggt gaccacggtt gcgtaatttc tttccgtgag ccgaaaacag gaatgtccgt
    22561 accaggtaat ttccataaaa gagcctccaa agtgcaatta tacccgatgg gtacgctcgc
    22621 cga
//
LOCUS       DAOQLC010000071        22121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_810_length_22121_cov_21.736382, whole genome shotgun sequence.
ACCESSION   DAOQLC010000071 DAOQLC010000000
VERSION     DAOQLC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 22121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_810_length_22121_cov_21.736382"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PL000_13070"
     CDS             <1..698
                     /locus_tag="PL000_13070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89879.1"
                     /translation="LHKMRKYQMDRITWLRELQRDCEAKYDGYAPLYWEKYGSYSNIT
                     HQRFIQEFLDHLSTNSTILDAACGAGRYEPFLLEKGHTVIGVDQSQGMLERAKEKFPN
                     VQYRKIGLQEIAYQEIFDGVICIDAMENVCPEDWMLVLNNFYHALKPEGYLYFTAETI
                     ENADENELKQAFERAQQGDLPVVYGEFPDEEVYHYHPSNQQVKEWAKQAGFEILREAN
                     GEIWYYHILVRKI"
     gene            914..1699
                     /locus_tag="PL000_13075"
     CDS             914..1699
                     /locus_tag="PL000_13075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89880.1"
                     /translation="MNKSQIIALYEQDQRKDLEYPNTQRKITPKVVRHVNASDIGGGF
                     ITYSHLNEADADDMIHEQVKYFESIGQDFEWKLYDYDQPSDLRQRLESYGFFIEEAEA
                     IMVLALEDAPEILWKPIRHNVQQIADPEKLVDVQSVEQQVWDEDYSWVLRSLGDALRK
                     YPEQMSVYVAYINEKPASAAWVYFSEHSQFASLWGGSTVSEFRHQGLYTALLAVRAQE
                     ARVRQVKFLTVDASPMSRPILEKLGFEMIAYSYPCKWKSKSKK"
     gene            1854..2783
                     /locus_tag="PL000_13080"
     CDS             1854..2783
                     /locus_tag="PL000_13080"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNC89881.1"
                     /translation="MQPDNASSPNIYINEDLVGQLIASQFSKWANLPLKPVEVSGWDN
                     RTFRLGNDMSVRLPSEAWYVAQVEKEQYWLPKLAPFLPVQIPIPLALGYPDKNYPWHW
                     SIYKWLEGENASIENIKNLHEFAISLAQFLVALHQIDSSGGPPPGEHNFFRGGALKVY
                     DDETRSSIFALRNEINFDAAAEVWEISLDTTWNKEPVWLHGDFASGNLLVNKGQLSAV
                     IDFGCLGIGDPACDLTIAWTFLSGKSRNAFIKTIALDDATWARARGWALWKALITLVE
                     HIEKDDNKSKYARYVINEVLEDHKYRGDTFKRI"
     gene            2970..4121
                     /gene="chrA"
                     /locus_tag="PL000_13085"
     CDS             2970..4121
                     /gene="chrA"
                     /locus_tag="PL000_13085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HNC89882.1"
                     /translation="MTDITKSDISQESYWQLFLRFLRFGLLAWGGPVAQIAMIRQELV
                     DEEKWISKERFNRVLAVYQVLPGPEAHELCVYFGMIARGRIGGLLAGLGFMLPGFVLM
                     FALSWVYVTYGINSPIFAAVFYGCKAAVVALIIRAIHRIGGHSLTDYWLLGIAIAVGL
                     ASLLGANFLFTLSLAGLAYSVIKLQSPPSVLHAFLPLPLFAQSAGILAPSLFALFGYG
                     LRSGLLTFGGAYTVISFLQYDAVVTGGWMTNAQFLDGLALSGILPAPLIIFATFVGYL
                     GGGAIGAIALTIGIFLPAFAFTLIGHEYVERLVENKSAHAFLDGVTAGVVGLIVVTAI
                     GLFREAVFDIPTIIIFALALLAVYRWKAKAAVVGIMFGAGLLGWLLFGV"
     gene            4213..4836
                     /locus_tag="PL000_13090"
     CDS             4213..4836
                     /locus_tag="PL000_13090"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC89883.1"
                     /translation="MSRFGSDPLSFFNSVYQNIAPWDIGTPQPAMAALIEKHPPANPV
                     LDLGCGSGDLAIYLAKLGNQVVGIDFVESAIKNAQDKVKSLPDETAQIIKFQVADALK
                     PSLLQDKFGAVFDSGFYHLFNPDQCEQLIDEVASILKPHGCYYLHEFAVEFSIPNVPR
                     QISIDELQTRFTVEKGWRIKEVQTVEFLSRVAPPVPAICACVERLPM"
     gene            5053..6441
                     /locus_tag="PL000_13095"
     CDS             5053..6441
                     /locus_tag="PL000_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNC89884.1"
                     /translation="MSNENIQAVENGLLAAVSIHGVPEKKHTLMDRMKYFCVPGLSIA
                     VINNGEIEWARSYGVLEAGKNQAVSSDTLFQSASISKPVSAMMALKLVEQDRLNLDQD
                     VNEILSSWKIPENEFTDTNKVTLRGILSHTAGLTVHGFQGYDFDGEVPSLLQMLDGLP
                     PANSDPIRVDVRPGSVFRYSGGGYTVLQQILEDVSHKTFDELLRIEILDQLNMPDSTF
                     EQPLPAHLRNKAATAHVDSGIPIQGKWHVYPEKAAAGLWSTPTDLAKFAIEIMNSCNN
                     RSNKMLSKNMADSMLTTIQGDYGLGLVVKHLENNIIRFGHSGGNVGFRCYFAAYTGSG
                     KGAVVMTNSDNGNFVAMEIIRSLSRIYEWSDFQPMEKQIDRQVGSDVYSQYVGKYQHP
                     EYSDFFVQIEKDGSGLFIEAIPGAPRWELYPESETRYFCVETETSIEFQKNNDGVFDA
                     LIYFSMELKKVV"
     gene            6620..6976
                     /locus_tag="PL000_13100"
     CDS             6620..6976
                     /locus_tag="PL000_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC89885.1"
                     /translation="MTERAEITDEFIQKRIMTGRQYCVRLYKVGPNRSQPQDEVEGIQ
                     KEHLRYLWQLRAEGKLLISGPVMDDSDLQGVGIFQSTDKEEVKKLCEGDPAVKAGRLV
                     YEIYSWFGLPGDSLPE"
     gene            7012..7527
                     /locus_tag="PL000_13105"
     CDS             7012..7527
                     /locus_tag="PL000_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89886.1"
                     /translation="MILPKKRDPRFITIRRGGTLQDIDHQLLALWAADCAQHVLYLFE
                     ETQLGDDRPRRAIEAARAWAQGKITMSQSKAAGGHAMAAARNLRGASRYAAYAAGQAG
                     VVAHVAAHELGAAAYAIKAVREAAPDGQVEEFGHLEWKWQCAQLPNRIRDLVLNDQEL
                     RNEICWFMFSH"
     gene            7777..8334
                     /locus_tag="PL000_13110"
     CDS             7777..8334
                     /locus_tag="PL000_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89887.1"
                     /translation="MKNTFAILFVFVLSACATVPISSSPEPPVSIETSPALTAEVPET
                     QVPAQSIYTSTKYGYSLSYPDTLNVIVVSDEYVEIGEKITVEVMNVDPTAPRGDGAVI
                     ETISDTQISGYPAKLLTGYIGSVGGYIPQQFRKVVVERNGSYFVVTLYALGLHVTDGD
                     LSKIAELAMVDISLFDNVTASMQIP"
     gene            complement(8460..9551)
                     /gene="serC"
                     /locus_tag="PL000_13115"
     CDS             complement(8460..9551)
                     /gene="serC"
                     /locus_tag="PL000_13115"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNC89888.1"
                     /translation="MSDLKRAYNFNPGPGALPLEVLQQAQAELLDFKGTGMSVMEISH
                     RSKEFEAVIQTAEADLRELLGIPSNYKVMFLQGGATLQFTMLAMNLRPAGASADYIVT
                     GTWSKTAIKEAKKLGAARAAANTEADGFNCTPASLDLDPKAAYLHFTSNETIHGVEFF
                     TEPTPPDGVPLVCDTSSNFISRPIDVSKYAFLYAGAQKNAGPSGVTVVIARDDMIERT
                     PENLPVMLDYKTLAASGSLHNTPPSFAIYMVGLVFQWAKKQGGLAAIEKTNRIKAGLV
                     YDAIDQSGGFYRGHAKKEDRSLMNIPFRLPSEELEETFAKEAKKNNLIGLKGHRSVGG
                     MRASVYNAVTVEGAKALVDFMKEFQKKNG"
     gene            9830..10729
                     /locus_tag="PL000_13120"
     CDS             9830..10729
                     /locus_tag="PL000_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNC89889.1"
                     /translation="MKILICDKTEQEYIEQMRAAGLTVDVRDDITPEELPNVLPAYDG
                     MVVRSRTKVRQALIDVCPNLKVIVRGGAGLDTIDHEYAKSKGIAVMNTPLANSKSVAE
                     LAIGYMLMMARSLYKASATMKAEKWDKKSFDGDEIGGKTLGLIGIGNIGKEVARRAIA
                     MDMTVVAYDPYVKEVEGIKLVSLDELLAQSDYISLHLPKTKESTGMIGAGQFAKMKKG
                     VRIVNCARGGIVDENALYDALISGQVGAAALDVFAEEPPVDWKLMKLDNVIASPHIGA
                     ATKEAQARVGAEVADKLIAFSKK"
     gene            10776..12092
                     /locus_tag="PL000_13125"
     CDS             10776..12092
                     /locus_tag="PL000_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HNC89890.1"
                     /translation="MKIISDIGIQIPQVYLPKPGTDLKKWAVIACDQFTSEPEYWHEV
                     EKIVGDAPSTLNLTFPEVHLEKEGGDQRIQNIQSAMRKYMDDGILQPRDGLIYVERTV
                     AGKTRKGVILCLDLERYDYTKGSSSLIRATEGTIVDRLPPRIKIREGAAMELPHILVL
                     IDDPNHTVIEPLSAAKSKLEKLYDFDLMLDSGHLAGYAVSEEFENKVVEALRGLAKPE
                     TFTAKYDIGKDQPVLLFAMGDGNHSLATAKAIWEKMKPQVGMDHPSRYALVEIENVHD
                     EGLEFEPIHRVLFNLKKDLFAELKKTFGDNFSYTEVASGEEMIQKVDSSEGQKQAIGL
                     VGGGRKFGVIEIAHASSNLAVGTIQAFVDSFLKEGGAEKVDYVHGEDVVERLSLQAGN
                     AGFYLAGMHKSDLFKTVILDGALPRKTFSMGEAREKRFYMEARKIS"
     gene            complement(12161..12814)
                     /locus_tag="PL000_13130"
     CDS             complement(12161..12814)
                     /locus_tag="PL000_13130"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC89891.1"
                     /translation="MQIIHKDEDILVINKPADLPVLPDGWDKDAPYLVKMLEADFPKV
                     WVVHRIDKSTSGIIIFALTAEAHRSLNIQFEKHLVEKTYHAILNGSPKWLDKITKFPL
                     RANVGNKHRTVVDNRNGVRAETRFRILKQNQLSALAEAQPMTGRTHQIRVHAYALGYP
                     LLGDILYSAPETNIISRAALHAYSLSFDHPTSNERLTFTAPYPADFEKALQLLKLAS"
     gene            complement(12798..13397)
                     /locus_tag="PL000_13135"
     CDS             complement(12798..13397)
                     /locus_tag="PL000_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89892.1"
                     /translation="MESWLTSTPYPGMILWIVLYISDYYMTLNSAKGFQEIGHFHFEG
                     SFELTPQFQEDINKQRRISRRHIIFLVVYTLVILVAWWLFAKTLKAEWLYSIYLGTLI
                     LMEVAVHLRHFRNLHMVKAIKNEGGVEGDITYQKKLSYRISANEFQTFAWLFFIVAVI
                     TYSPFFLGGVISCFGISFRHNAYAKRAAGTPKAVHADHS"
     gene            13480..14226
                     /locus_tag="PL000_13140"
     CDS             13480..14226
                     /locus_tag="PL000_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC89893.1"
                     /translation="MFTVKQLSTLAGVTPRTLHHYDEIGLLKPSRIGENGYRYYGEDA
                     LLKLQQILFYRELDIPLDEIKKIMGRRDFDVLGALQSHKDALNKQAVRINRLIQTVDN
                     TIQHLKGKTKMSEEGLFEGFSEEVQEKYAREAEQMYDPETVRESNRKWKGYSAAKKEA
                     ILAEGKAVYMDMVAAMPKGAASAEVQAIVERWRKHMDYFWTPSLDQLPGLADGYSSDP
                     RFKANFDKMHPNLAEFMREAVKVYVEKHSK"
     gene            complement(14263..15048)
                     /locus_tag="PL000_13145"
     CDS             complement(14263..15048)
                     /locus_tag="PL000_13145"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC89894.1"
                     /translation="MQTLKLLSAFLKMNIQMSLAYRADTVVNIALNLMWLGWELLSLN
                     IIFNNTETLGGWGLGELIALLGVFRLVNTMMIAFIWPNTEKFNQSIRDGSMDYTILQP
                     VNSMFLVTFSRITVWRFWDLILAVVLIVVGINMSGDTTSALQILTFILLTITGMLVIY
                     SLWIILIAMTFWFTKFDNNVTILQALLDAGRYPATVYPVWLRVIVTFIIPIAVATTVP
                     LQALRGDLTLVNILIFTAIGFISFGIASQVWKAGLKRYSGASS"
     gene            complement(15052..15849)
                     /locus_tag="PL000_13150"
     CDS             complement(15052..15849)
                     /locus_tag="PL000_13150"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC89895.1"
                     /translation="MLLIFKRLWQVNWAEQWQYRANLVMYLLYWLVSPIIYLAVWTSI
                     ANAKGDVSGFTANDFITYYMLIIICDQLTSNIFIHIFAYKIQDGTLSGELTKPIHPVL
                     TNSLVVNIANKILNVIVLIPVWAILFLIFKPDFSSVTLQGILLSIPVMAVSFLIGYLL
                     SATITSLAFWTTRVYSIHEFYFALILLFSGQFVPLPLMPKVIQDIAQYLPFQLLLYFP
                     VQLILGKLNTNQIVQGYIMAGVWLAVSILLFNWVWRNGVKRFSAVGA"
     gene            complement(15927..16904)
                     /locus_tag="PL000_13155"
     CDS             complement(15927..16904)
                     /locus_tag="PL000_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC89896.1"
                     /translation="MSPTINVQQLSKTYQVPEREGGFGAAVRSFFKRKYKDVKAVQQV
                     NFKIEQGEIVGFLGPNGAGKTTTLKMLSGLLHPTGGKAEVLGFTPWELKPEYLRVMTL
                     VMGQRNRLSWDIPAADSFLLNQAIYRIPDDEYKKTYKELDELLELEPLMKKPVRNLSL
                     GERMKCEIAAGLLHRPKILFLDEPTIGLDITGQARIREFLREYNKRTGATILLTSHYM
                     ADVTALCERIIIIHHGSLKYDGGINDLSKKIAPFKLIGVLLAESNSHDLSKYGEPVQN
                     EEDAEKQYIQVKAEDVTRVTSRLLADLPIHDITIADPPIEDVIEQAFNQ"
     gene            complement(16959..17603)
                     /locus_tag="PL000_13160"
     CDS             complement(16959..17603)
                     /locus_tag="PL000_13160"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNC89897.1"
                     /translation="MTTKPDTIKISIMEKEEISATHADLHVTIKGTSLVSGSEAMKKA
                     KEVNQLVEALTSLGLKSEAFFLQGVHLEQAGGALLKSSSAVYRLKIRCEDLEKLSAIL
                     DGISAQKNATLERIEWRYDDEETRRQLLNRALENAKKKAQQVADTLRVKLIGVYDFLE
                     TPMDDEAPRFQAQSAPVFRGAPAPAEQGLGMDIQHTKTLHMRVDIWYRVSELQD"
     gene            complement(17650..18921)
                     /gene="kynU"
                     /locus_tag="PL000_13165"
     CDS             complement(17650..18921)
                     /gene="kynU"
                     /locus_tag="PL000_13165"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HNC89898.1"
                     /translation="MSFSTAREFAQQLDTHDRLAAIRNEFVIHDPNLIYLDGNSLGLL
                     PKAAQAKARQVVEEQWGVDLIRGWNKGWWDSPARVGDKIGQLIGAAAGQVLVNDTVSL
                     NLFKLAAAALTLQPSKTRIITDTFNFPSDLYILQGIRQTFGGRHEIISIGASDNDITP
                     DLAALETAIDENTALVTLSHVTFKSGYLYDMQRITELAHKKGALVLWDLSHSVGALPI
                     HLDACNADLAIGCTYKYLNGGPGAPAFLYVNKSIQEKLSSPIWGWWGQNDPFDFSLNY
                     QPARGAERFLVGTQPILSLLTMEAALEPTLQAGMDSIREKSILMTGYASFLTESWLAP
                     FGFSLGSPPDPAKRGSHISIRHADGYRINRALIEEMNVIPDFREPDNIRLGFAPLYTS
                     FTDVWEGFERIKRVMEEKRHEKYSKQKLTVT"
     gene            complement(18928..19518)
                     /locus_tag="PL000_13170"
     CDS             complement(18928..19518)
                     /locus_tag="PL000_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89899.1"
                     /translation="MPESIMEYWKFDEADLGANRLGQLTEKQKKYLVGEHKSQRSVFL
                     GVGGVVAFLFLCLPALLIGSRVVLPMLLNGDLSDLQDILPLAAMGGFGIVFTGIAVLT
                     VGAVVAIYVMRASKPAEITVTTARGKVEYTWGTKRVSTPGSSVRSYEDVKVLHLNLGD
                     KKFEVKESLQELIKEGEDWSVYYTSYPFKFLSAEKK"
     gene            complement(19555..21162)
                     /locus_tag="PL000_13175"
     CDS             complement(19555..21162)
                     /locus_tag="PL000_13175"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNC89900.1"
                     /translation="MSQIYDYVIIGSGFGGSVSAMRLAEKGYSVLVLEKGKRFNDKDF
                     ATSNWQYWKYVWIPALRAHGILQIAILKGVMVLHGAGVGGGSLGYANVLEVPNEETFA
                     TPAWNQNIKWGDVLRPHYETAKKMLGAARNPKLWKADLLLKDFAEERGMGHTFRATDV
                     GAFFGEAGVTVPDPFFGGEGPNRAGCQHCGGCMVGCRHNAKNTLPKNYLYFAEKKGAQ
                     VKAELEVMDVKPLPTALETGARYEVHYRPSTSLLKSQHIVHARNVIFSAGVMGTMKLL
                     LNLRDVKKSLPKLSARLGTMVRTNSEGLLGSVARRSDINYSEGVSISSIYNHDEITRI
                     EPVRYPDGSSLMRFLAAPLIDNNASIPMRIIKFLGWALTHPIDFAKAAFLPGWAHNVT
                     ILLVMQNADNRMRFRMGRSLFTLFRTGLVADEEPGFKINAQVKGSHEITREFAKRTNG
                     VALGSIGENLLGLPTTAHILGGAPIGKSPEEGVVNENFAVHNYPGLYIIDGSIMPANP
                     GVNPSLTITALAEYAMSKVPPRSATTD"
     gene            complement(21224..22033)
                     /locus_tag="PL000_13180"
     CDS             complement(21224..22033)
                     /locus_tag="PL000_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNC89901.1"
                     /translation="MDKTILEIPTDLLEAAKLTADEAKTELAIRLYQLHKLNDEQAGV
                     LAGNPKLIEEMAWNNRSTGRFDMDEFLSWASHDLKTPLNAIIGFTRVVMKGMDGPVND
                     IQTTDLTTVYNGGQRMLALVGNLVDMARLNNGEITLNREPCELRSILVNTADQWQVKN
                     PNKNLTTDIIIHEPVYNIDLARVNQIVTSLLSLAATRVVEGSLSLSARDNDNGAVITV
                     QSTGRKSRDKFEMDSAMLGFISSSLIKLHGGNTESVEETEDGMMIVFHLPR"
BASE COUNT         5488 a         5052 c         5774 g         5807 t
ORIGIN      
        1 cgcttcacaa aatgaggaag tatcagatgg atagaatcac ttggttaaga gaattacagc
       61 gtgactgtga agcaaaatac gatggttatg ccccgctcta ctgggaaaaa tacggctcgt
      121 acagcaacat cactcaccag cggtttattc aggagttcct agatcatctc tccactaaca
      181 gcacaattct tgatgcagct tgtggggctg gcaggtatga gcccttcctt ctggaaaagg
      241 ggcatactgt cataggggtt gatcaatcac aaggtatgct tgaacgtgcc aaagagaaat
      301 ttcccaatgt ccagtatcga aagatcgggc tgcaagaaat tgcatatcaa gaaatcttcg
      361 atggcgtgat ttgcatagat gctatggaaa atgtatgtcc cgaagattgg atgctcgtac
      421 tcaataattt ttatcacgcg ctcaagcccg aaggttatct ctatttcact gcggaaacaa
      481 ttgaaaatgc agatgagaat gaactcaagc aagccttcga gcgagctcaa caaggtgatc
      541 tgccagttgt atatggagag tttcctgatg aagaggtgta tcactaccat ccgagtaatc
      601 aacaggtgaa ggagtgggct aaacaagcag gatttgagat tttgcgagaa gcaaatggcg
      661 agatatggta ttaccatatc ttggttcgaa agatttaggt ttatcgaaaa tgctaaacag
      721 cgcctaacaa agcatgcatt tgacgggtgg gattctgcag cattttcggg ctttatctac
      781 acccgaatag aatcctgctc tcggagtttt gtccacgccc gcccacctgc cagtagcgca
      841 aaccgttagg tggctgtgct agtcaatcat ttattattat cttcggttgt tgaacgagtg
      901 agagaatcct tccatgaata aatcacaaat tattgccctt tacgaacaag atcagagaaa
      961 agatttggag tatcccaaca cgcaacggaa aatcacgcca aaagtggtac ggcacgtcaa
     1021 cgcttccgat attggaggag gatttattac ttacagtcat ttgaatgaag ccgatgcaga
     1081 cgatatgatt cacgagcagg tgaaatattt tgaaagcatt ggacaggatt ttgaatggaa
     1141 actttatgat tatgatcagc catctgactt gagacaaaga ttagagtcat atgggttctt
     1201 catagaagaa gcggaagcca ttatggtact tgccttagaa gatgctccag agattttatg
     1261 gaagccaatc cgccataacg tgcagcaaat cgcggatcca gaaaagttag ttgatgtcca
     1321 atctgttgaa caacaagttt gggatgaaga ctattcctgg gtgctccgtt ccttgggaga
     1381 tgccctcaga aagtatccag aacagatgag cgtgtatgtg gcatacatca atgaaaagcc
     1441 tgcaagtgca gcctgggtat atttttcaga acacagtcag ttcgccagcc tgtggggagg
     1501 ctctacagta agtgagtttc gccatcaagg gctatatacg gcgctcttag cagtgcgagc
     1561 gcaggaagcc agagtgcgtc aggtaaaatt tcttacagtg gacgcctcgc ccatgagtcg
     1621 tcccattctg gagaagctcg gttttgaaat gatagcctat tcttatcctt gtaaatggaa
     1681 aagcaaatct aagaaataat ggtaatgggt cgccacctaa aaaagcacgg atatgacgct
     1741 ggggactgac gtaaggcgtg cacacacctc aagcattttt ccagtcggac ggattcgcca
     1801 accaagtagc gcaacccgtt agacatttcc ttccaaaatg atatggagta catatgcagc
     1861 ctgacaatgc atcttctcct aatatttata ttaacgaaga ccttgtaggt caattgattg
     1921 catcgcagtt ttcaaaatgg gcaaaccttc cacttaagcc agttgaggtt agtggatggg
     1981 ataatcgaac ctttcgtctt ggtaacgata tgagtgtaag gcttcccagt gaagcatggt
     2041 atgttgcgca agtggaaaaa gaacaatatt ggttgccaaa acttgcacct tttctcccag
     2101 ttcaaatccc tattccattg gcacttggct atcctgataa aaattatccg tggcactggt
     2161 caatctataa atggcttgaa ggcgagaatg cttcgattga aaatattaaa aatcttcatg
     2221 agtttgcaat ttcacttgct caattccttg tagcacttca tcaaattgat tcgtctggcg
     2281 gtccgccgcc tggagaacat aacttttttc gtggcggagc actcaaggtg tatgatgatg
     2341 aaactcgcag ttcaattttc gctctaagaa atgaaataaa cttcgatgcg gctgccgaag
     2401 tttgggaaat atcccttgac acaacatgga ataaggaacc cgtttggttg catggtgatt
     2461 ttgcatcagg aaatttactg gtaaataaag gacagttaag cgcagttata gattttggat
     2521 gtttgggaat tggcgatcca gcatgtgatt tgactatcgc atggacgttt ttgtcaggaa
     2581 aaagtcgaaa tgcgtttatc aaaaccattg ctctagatga cgcaacctgg gcgcgtgctc
     2641 gtggttgggc actttggaag gcgttaatta cacttgttga acatattgaa aaagatgata
     2701 ataaatcaaa atatgccaga tatgtaatca atgaagtgct tgaggaccac aaatacagag
     2761 gtgatacctt caaaaggata tgagtctaaa aaataaaaaa cgcctaataa agtatttacc
     2821 tgatgctggg ggcttcccat aggcatgtgc atatcccaag cagctttctg gctggacggc
     2881 ttttccgtct ccgccccagc gcaggtaacg catgccgttg gcacgccctt gcataacaat
     2941 ctcattttat tgttaaaata ggatactcaa tgactgacat aaccaaatct gacatttcgc
     3001 aagaatctta ctggcaactt ttcctccgtt ttctgcgctt cggtcttttg gcttggggtg
     3061 gtcccgtcgc ccagattgct atgatacgcc aggaattagt tgatgaagaa aagtggattt
     3121 caaaagaaag attcaatcgt gttttagccg tttatcaagt tttgcctgga cccgaagccc
     3181 atgaactttg tgtttatttc ggcatgatag caaggggacg tattggcggt ttgttggcag
     3241 ggcttgggtt tatgctcccc ggctttgtgt tgatgtttgc actatcctgg gtgtatgtta
     3301 cttacggcat taactcacct attttcgccg ccgtatttta tggatgtaaa gccgctgttg
     3361 tagccctcat cattcgcgcc attcatcgca tcggtggaca ttctttaact gactattggc
     3421 ttttgggtat tgctattgct gtagggcttg ccagtttgct gggcgccaat ttccttttca
     3481 ccctttctct ggctgggctt gcttattcgg tcatcaagct tcaatcgccg ccatctgtct
     3541 tacatgcttt tttacctcta ccattattcg cccaaagtgc tggaatactc gccccttcac
     3601 tattcgcatt gttcggttat ggcctccgaa gcggtttatt gactttcggc ggtgcataca
     3661 cagtcatttc gttcttgcaa tatgacgcag ttgtaactgg cggttggatg accaacgctc
     3721 aatttttaga tggattggcg ttgtcgggca ttcttcccgc tccgcttatt atcttcgcta
     3781 cttttgtcgg ttatcttggc ggcggcgcaa ttggcgcaat tgcgttaaca attggaatat
     3841 ttttacctgc ttttgctttt actcttattg gtcacgaata tgttgaaagg ctcgtagaaa
     3901 acaaatctgc ccatgcattt ttagatggcg tcaccgcagg agtagtaggg cttatcgttg
     3961 ttacagcaat tggactgttt cgagaagccg tttttgatat accaacaatc attatttttg
     4021 cgctggcgtt attagcggtg tatcgttgga aagcaaaagc cgcagttgta ggtattatgt
     4081 ttggtgcagg cttgttgggt tggctattgt ttggcgtgtg agttttgagg tcaaaagtgt
     4141 gccgaggaag catgcgtctg accctttgga ttgccgtaag gcgcttaacg aaaacataca
     4201 aggagatttg ttttgagtag atttggttct gacccacttt cttttttcaa ttctgtttac
     4261 cagaatattg cgccttggga tattggtacg cctcaacccg caatggctgc tcttattgaa
     4321 aaacacccgc ccgcaaaccc agttttggat ctcggatgcg gctctggcga cctcgccatt
     4381 tatttggcaa aactgggtaa tcaagttgtt gggattgatt ttgttgagtc tgccatcaag
     4441 aatgcccaag ataaagttaa gtctcttccc gatgagacgg cccaaataat aaagtttcag
     4501 gtggcagacg ccctaaagcc atctttactt caagataaat ttggagcggt ttttgattca
     4561 ggtttctatc atttatttaa tccagaccaa tgcgaacaat taattgatga agttgcctca
     4621 atcttgaaac cgcatggttg ttattatttg catgagttcg cagtcgaatt ctctatacct
     4681 aatgtgcctc gccaaatttc aattgacgaa ttacaaacac gtttcactgt tgaaaaaggt
     4741 tggcgcatta aggaagttca aacggtggaa ttccttagtc gggtcgcacc tcccgtgcca
     4801 gcgatctgcg cttgtgtaga gcgccttcca atgtaaacca cacctgacaa aacatgcacg
     4861 gggcttctat gccgtatcat tttttgattg gatggctttg ccgtctccgt cccagcacag
     4921 ataacgcaaa ccgttgagcg gcttcgcgta ctctcacaat attttcatgc agaagttaat
     4981 tcccatgagt gagtgaaata aaagcgttgt ttgaagacaa agcatgcaga accaatagaa
     5041 aaggtgatag acatgtcaaa tgaaaatatt caagctgttg agaatggttt gttggcggcg
     5101 gtttccattc atggggttcc cgaaaaaaaa cacaccctga tggatagaat gaaatatttc
     5161 tgtgttcctg gtctaagcat tgccgtgata aacaatgggg agattgagtg ggcgcgcagc
     5221 tatggtgtgt tggaggcggg taagaatcag gcagtttcat ccgatacatt gttccagtca
     5281 gcctcaatta gtaagcctgt ttcggcaatg atggcgctca aacttgtaga gcaggacaga
     5341 ttgaatttag atcaagacgt aaatgaaatt ctttcttcat ggaagatccc agagaatgag
     5401 tttacagaca caaacaaggt aactctccgc ggcattcttt cacacactgc tgggctaaca
     5461 gttcacggct ttcaaggtta tgattttgat ggtgaagtac catcattgtt gcaaatgctt
     5521 gatgggctgc caccggctaa ttcagatccg atacgtgtgg atgttagacc tgggagcgtg
     5581 ttcagatact ctgggggtgg atatacagtc ttgcagcaaa ttcttgaaga tgtatcccat
     5641 aaaacgttcg atgaactgtt gcggatcgaa atattggatc agttaaatat gccagacagc
     5701 acatttgaac aaccacttcc agcgcatttg cgaaataaag ctgcaacggc gcatgttgat
     5761 tcaggcatac ccatccaggg gaaatggcat gtatacccag aaaaagcagc cgccggttta
     5821 tggtcaacgc ccacagacct ggcaaaattt gccattgaga tcatgaattc ttgtaacaac
     5881 cgttcgaata agatgttatc aaaaaatatg gctgacagca tgcttacgac gatccaggga
     5941 gattatggat tgggtttggt ggtaaagcat ttggaaaata atatcattcg ttttggtcat
     6001 agtggtggga atgttggttt ccgttgctac ttcgcggcct acacaggcag cggcaagggt
     6061 gccgttgtga tgacaaatag tgacaacggc aattttgtag caatggaaat tattcgcagc
     6121 ctctcacgca tctatgagtg gtcggatttc cagcctatgg aaaaacaaat tgaccgtcag
     6181 gttggttctg atgtttattc tcagtacgtg ggaaagtacc aacatcctga gtattctgac
     6241 tttttcgttc aaatagaaaa ggacggatca ggcttgttta ttgaggcaat tccaggtgcg
     6301 ccacgctggg aactttatcc cgaatcggaa accaggtatt tttgcgttga gaccgaaaca
     6361 agtattgagt tccagaaaaa caatgatggc gtttttgatg ccttgattta tttctcaatg
     6421 gagttgaaaa aggttgtctg attggaattt cccgacgaag cagtgcggtc tttacctcct
     6481 gaaagaggat tttgtaccct tggtcagatt ctacccgtaa ctttttgcta acccccacca
     6541 gtaagactcg ctattggtgg tttttattaa gtacccgtat gggacaaatg agactcttat
     6601 agagaaaggt tgcaggcgaa tgacagaaag agctgaaatc acagatgagt tcatccaaaa
     6661 aaggatcatg acaggaagac aatattgtgt ccgcttatac aaagtagggc caaatcgtag
     6721 tcagcctcaa gatgaagtgg aaggcatcca aaaggaacat cttcgatacc tgtggcagct
     6781 tcgggccgaa ggaaaattgt taattagtgg acctgttatg gatgactctg accttcaagg
     6841 tgtaggtatt ttccaatcca cagataaaga ggaggtaaaa aaactgtgtg aaggagaccc
     6901 ggcagttaaa gcgggtcggt tggtatatga aatctactcc tggtttggtc tgcccggcga
     6961 ttctttgcca gaataactct ccgggcagag gaaaatacag gatattaaag aatgatattg
     7021 cccaaaaaac gcgaccctag atttatcacg atacgccgtg gcggcactct tcaggatata
     7081 gaccatcaat tgcttgcgct atgggcagcc gactgcgcac aacatgtttt gtatctgttt
     7141 gaagaaactc agcttgggga tgatcgtccg cgtcgagcca ttgaagctgc gcgcgcttgg
     7201 gcgcaaggca agattacaat gtctcaatcc aaagcagcgg gtggtcatgc gatggcagct
     7261 gccagaaatt taagaggagc atctcgctat gctgcatatg ctgctggtca ggcgggtgta
     7321 gttgctcatg tggcggcgca tgaattgggg gcggccgcct atgcgattaa ggcagtacgc
     7381 gaagcagccc cagatggtca ggttgaagaa ttcggccatc ttgagtggaa atggcagtgc
     7441 gcgcagctcc cgaataggat ccgtgacttg gtgcttaatg accaggaatt gcgcaatgaa
     7501 atctgttggt tcatgtttag tcattaggaa aaccttgtcc catagggttc atatgggagt
     7561 tgcgaaattt cggttcgaaa gtgctcttgt ttcgctgtct taggatggat aaatatttga
     7621 tttccccctc aaaatggagc ttttacagtg aattgcttct gaatcgtatc tgtttcaatg
     7681 tagataatat agaccattgg gggactttat caaatccgtc attcaattag aatcgcgcaa
     7741 tggtcaataa ttttcaaata ttaaaaaaag gaacttatga aaaatacgtt tgcgatcttg
     7801 tttgtattcg ttctatcagc ctgtgctact gtgcccattt cctcctcgcc tgaaccgcca
     7861 gtttcaattg aaacttctcc tgctcttact gctgaagtgc ctgaaaccca ggttcctgca
     7921 caaagcatct acaccagtac aaaatatgga tattctttga gttatccaga cacactgaat
     7981 gtcatcgttg taagcgatga gtatgttgaa attggcgaaa aaattacagt tgaggtaatg
     8041 aatgttgacc cgactgcgcc tcgcggagat ggagccgtca ttgaaaccat ctccgatacc
     8101 caaatttcag gttatcctgc taaattatta acggggtata taggctctgt tggtggttat
     8161 atccctcaac agtttcggaa ggttgtagtt gaacgaaatg gcagctattt tgtcgtcact
     8221 ctttatgcgc ttggcttaca tgttacagac ggcgatcttt cgaaaatcgc agaacttgcg
     8281 atggtagata tttctttgtt cgataatgta acggcatcca tgcagatacc ctagttccat
     8341 cttcgaatgc tccactctcg ctggggaaga gtttttggtg tcgaaggcct tgccaaaaat
     8401 tcaaaaaagc ctttgagttt tcgctcaaag gctttttaat ttattgtttc cttgttttat
     8461 tatccgtttt tcttttgaaa ctctttcatg aaatcgacca atgccttggc accttcaacg
     8521 gttacagcat tgtaaacgga tgcacgcata ccgccgacag accgatgacc cttgaggccg
     8581 atcaggttgt tcttcttggc ttccttggca aaggtctctt cgagttcttc actgggcaga
     8641 cggaagggga tattcatcag tgaacggtct tccttcttgg cgtggccgcg ataaaagccg
     8701 ccgctttggt caatggcatc gtaaaccaga ccagccttga tgcgattggt tttttcaatg
     8761 gcagcgaggc cgccttgttt tttagcccac tgaaagacga gaccaaccat gtagatcgcg
     8821 aaactgggcg gtgtgttgtg cagtgatccg cttgcagcga gagttttgta atccagcatc
     8881 acgggcaggt tttcgggggt gcgctcgatc atgtcatcac gggcaatgac cacggttacg
     8941 ccagaggggc cagcgttttt ctgtgcgcca gcatacagaa aggcatattt cgatacatca
     9001 atggggcggc tgatgaaatt ggatgaagta tcacaaacca gcggaacgcc atcgggcggg
     9061 gtgggttcgg tgaaaaattc cacgccgtga atggtttcgt tggaggtaaa gtgcaaatat
     9121 gcggccttcg ggtcaaggtc aaggcttgcg ggggtgcagt tgaaaccatc cgcttcagta
     9181 ttggcagcgg cgcgcgcggc accgagcttc ttcgcttcct tgatcgcggt cttgctccag
     9241 gtgcctgtca caatgtaatc ggcagaggca cccgcagggc gcaggttcat cgccagcatc
     9301 gtaaattgca aagtggcgcc gccttgcagg aacatcacct tgtaattcga tggaatgccc
     9361 agcagttcac gcagatccgc ctctgcagtc tggatgaccg cttcaaattc ctttgagcga
     9421 tggctgattt ccatcaccga catgcccgta cctttgaaat cgagcaattc tgcctgtgct
     9481 tgctgcaaca cttcaagcgg caacgcgcca ggaccggggt tgaaattgta cgcacgtttg
     9541 agatcagaca taaggttact cctgttcact atgtggaagg ttttttacgg ttcttatttg
     9601 accgttggac tggttcggtt tttctgatac attcacttca ttataaatgg ttgtcagaca
     9661 aatctacaag aacaatcggc atgattccct actgatgtat gtcgttgaca aaggtttttc
     9721 tcgttgttag aatgaccagc gtgttgaaag taaccttcca aacaggaatt cagccgattc
     9781 gtcggttggg ttcctgttat taatttctgc cccaatcaag gagaaaccca tgaaaatctt
     9841 aatttgcgac aagaccgaac aggaatatat cgagcaaatg cgtgccgcag gtctcaccgt
     9901 ggatgtgcgc gacgacatca cccccgaaga actgcccaat gtattgcccg cttatgatgg
     9961 catggttgtg cgttcacgca cgaaagtgcg ccaggcactg atcgatgtct gccccaacct
    10021 caaagtgatt gtccgtggtg gcgcgggcct cgataccatt gaccatgaat atgccaaatc
    10081 caagggcatt gcggttatga ataccccgct ggcaaattcc aagtctgtgg ctgagctggc
    10141 catcggttac atgttgatga tggcgcgttc actctacaaa gccagcgcca ccatgaaagc
    10201 cgaaaaatgg gataaaaaat cctttgacgg tgatgagatc ggcggcaaga ccctcggcct
    10261 gatcggtatt ggcaatattg gcaaagaagt tgcccgccgc gccatcgcca tggatatgac
    10321 cgtggtcgcc tatgatccct acgtcaagga agtggaaggc atcaaactgg tctccctcga
    10381 tgaactgctt gcccagtctg actacatcag cctgcacctg cccaagacca aagaatccac
    10441 aggcatgatt ggcgcaggtc aatttgcgaa gatgaagaag ggtgtccgca ttgtcaattg
    10501 cgcgcgcggt ggaatcgtcg atgagaacgc tctctacgac gcattgatca gtggtcaggt
    10561 tggagcggcc gcgctggatg tcttcgccga agaaccgccc gttgattgga agctgatgaa
    10621 gctcgataac gtcatcgcat ccccgcacat tggcgcagcc accaaagaag cccaggcccg
    10681 cgtcggcgcg gaagttgccg acaagttgat cgcgttttcg aagaaataat cttgtaggga
    10741 tacggcgtcg ccgtatccct tactgaggag aagacatgaa aatcatttcc gacatcggca
    10801 ttcaaattcc gcaagtgtat ctccccaaac caggcaccga cttgaaaaag tgggcagtga
    10861 tcgcctgcga tcaattcacc tccgagcccg agtattggca tgaagtggaa aaaatcgtcg
    10921 gtgatgctcc ttccacgcta aacctgacct tccccgaagt gcatctcgaa aaagaaggcg
    10981 gcgaccagcg cattcaaaac attcaatccg ccatgcgcaa atacatggac gacggtatcc
    11041 tccagccgcg tgatggcttg atctatgttg aacgtaccgt ggcgggaaaa actcgcaagg
    11101 gcgtgatcct ctgcctcgac ctcgaacgtt acgattacac caaaggctca tcgagcctga
    11161 tccgcgcaac cgaaggcacc atcgtagatc gccttcctcc gcgcatcaag atccgcgaag
    11221 gcgccgcaat ggagttgccg cacatcctcg tgttgatcga tgacccgaat cacactgtca
    11281 tcgagccgtt gtctgccgcg aagtcgaaac ttgaaaaact gtatgatttc gatctcatgc
    11341 ttgatagtgg tcatcttgca gggtatgccg tcagcgagga attcgaaaat aaagtcgtcg
    11401 aggctttgcg cggacttgcc aagcctgaaa ccttcaccgc caagtatgac atcggcaaag
    11461 atcagcccgt actccttttc gcgatgggtg atggcaatca ctcgcttgcc acggccaaag
    11521 ccatttggga aaagatgaag ccgcaggtcg gcatggatca tccctcgcgc tatgcgttgg
    11581 ttgagatcga aaatgtccac gatgaaggcc ttgagtttga gccgatccat cgcgtgttgt
    11641 ttaacttgaa aaaagatttg ttcgcggagt tgaagaaaac cttcggcgat aacttcagct
    11701 acactgaagt cgccagcggc gaagagatga ttcaaaaggt ggactcttcc gagggtcaga
    11761 agcaggcgat cggtctggtt ggaggcggtc ggaaatttgg cgtgatcgaa atcgcgcacg
    11821 cgtcatccaa tcttgcggtc ggcaccatcc aggctttcgt cgattcattc ctgaaagaag
    11881 gcggcgccga aaaagtggat tatgttcacg gtgaagatgt tgtcgagcgc ctgtctttgc
    11941 aggcgggcaa tgcaggcttt tatcttgcgg gcatgcacaa atccgatctg ttcaagaccg
    12001 tgatcctcga tggcgcgttg ccgagaaaaa ctttttcaat gggtgaggcg cgtgaaaaac
    12061 gcttctacat ggaagcaaga aaaatttcat aattcgtgtt gaaagttgaa agttgaatgt
    12121 tgcaggttgg caggttttaa cttgtcaacc tgcaaacttt ttaacttgcc aacttcaaaa
    12181 gttgcaacgc tttttcgaaa tcagcagggt agggggcggt aaaagtaagc cgttcatttg
    12241 atgttggatg atcaaacgat aatgaatacg cgtgtagtgc ggcgcgtgag atgatattgg
    12301 tctcaggggc gctgtagagg atgtctccca aaagcggata gcccaacgcg taggcatgga
    12361 ctctgatctg atgcgttcgc cccgtcatcg gctgcgcctc tgccaaagcc gaaagttgat
    12421 tctgtttcaa gattctgaat ctcgtctcag cacggactcc attccgatta tccacaacgg
    12481 ttcggtgttt gttgcccaca ttggcgcgca gcggaaattt ggtgatcttg tcgagccatt
    12541 ttggtgagcc gttcagaatg gcgtgatagg ttttttctac aaggtgtttt tcaaattgga
    12601 tgttcagcga gcggtgcgct tcggcagtca atgcaaagat gatgatgccg ctggtgcttt
    12661 tatcaatacg atgcacgacc cagacttttg gaaaatccgc ttcaagcatt tttacaagat
    12721 agggagcatc cttatcccaa ccatcgggca atacaggaag gtcggcgggt ttattgatta
    12781 caagtatgtc ttcgtcttta tgaatgatct gcatgtacag cctttggtgt tcccgccgcg
    12841 cgctttgcgt aggcgttgtg tctgaaactg atcccaaaac aagagataac cccgccaagg
    12901 aaaaatggac tataggtaat gacggctaca ataaaaaata gccatgcaaa ggtttgaaat
    12961 tcgttggctg aaattcggta ggagagtttt ttctgatagg tgatatcacc ttccactccg
    13021 ccttcgtttt ttatggcttt caccatgtgc aaattgcgga aatgacgcaa gtgtacggcc
    13081 acttccatca ggatcagcgt gccaagatag atggagtaca gccattcagc cttgagcgtt
    13141 ttggcgaaca accaccaggc caccaaaatg accagcgtgt ataccaccag gaaaatgatg
    13201 tgccttcttg aaattcttct ttgcttatta atatcctcct gaaattgcgg cgtgagttcg
    13261 aaactgcctt cgaaatgaaa atgaccgatt tcctgaaatc cctttgcgga attcagggtc
    13321 atgtaataat cgctgatgta gagcacgatc catagaatca tgcctgggta aggggtggat
    13381 gtgagccagg attccataaa aatactccgt ttggcttatt gtacctgcta tccaaaaaat
    13441 ccttgacatt aacgtaacgt cagggtttat agtagcgaca tgttcacagt caagcagctt
    13501 tccactctgg ctggcgtcac tccgcggacg ctgcaccact acgacgagat cgggctcctc
    13561 aagccatccc gaattggcga gaatggctat cgctactacg gggaggatgc cctgttgaag
    13621 ttacagcaga tcctgttcta tcgcgaactg gatattccac tggacgagat caaaaagatc
    13681 atgggacgcc gtgattttga tgtgcttggc gcgctgcaaa gccacaagga tgccttgaac
    13741 aaacaggcgg ttcggataaa tcgcctgatc caaactgtgg ataatacgat tcaacatttg
    13801 aaaggaaaaa cgaaaatgag cgaagaagga cttttcgaag gctttagtga agaagtgcag
    13861 gagaagtacg ccagagaagc cgagcaaatg tacgaccccg agaccgtacg cgaatcaaat
    13921 cgcaagtgga aggggtattc cgcggcgaaa aaggaagcca ttctggcgga aggcaaagcc
    13981 gtttacatgg atatggtcgc tgcgatgcca aaaggcgcgg cctcggctga agtgcaagcc
    14041 atcgtcgagc gctggcgcaa acacatggac tacttctgga cgccgagcct tgatcaattg
    14101 cctggcctgg ctgatggcta ctcctctgac ccacgcttca aagccaattt cgacaagatg
    14161 cacccgaatc tcgctgagtt tatgcgcgag gctgtgaagg tgtatgtcga aaagcattca
    14221 aagtaaaaaa caggcgacca gaatctctgg tcgcctgttt ttttagctgc tcgcgccgct
    14281 gtatcttttg aggccagcct tccagacttg tgaggcgatc ccaaaactga taaacccgat
    14341 ggccgtaaag atcaggatgt tgacaagggt caggtcaccg cgcagggctt gcagcggcac
    14401 cgttgttgca acggcaatgg ggatgatgaa cgtgacgatc actcttaacc agacagggta
    14461 caccgtggcg ggatatcggc ctgcatccaa cagtgcctgc aggatggtga cgttgttatc
    14521 gaatttggtg aaccagaagg tcatcgcgat caaaatgatc cacaggctgt agatgacgag
    14581 catgcccgtg atggtgagca ggataaaggt caggatttga agcgcggatg ttgtgtctcc
    14641 gctcatgttg atccccacca cgatcagcac gaccgccaga atgagatccc agaaacgcca
    14701 gacggtgatg cgtgaaaaag tgaccagaaa catgctgttg actggctgga ggatggtgta
    14761 atccatggag ccatcgcgga tgctctggtt gaatttttcc gtgtttggcc agatgaacgc
    14821 gatcatcatg gtgttgacaa gccgaaagac acccagcagt gcgatcaatt caccaaggcc
    14881 ccaaccgccg agggtttctg tgttgttgaa aatgatgtta aggctaagta attcccagcc
    14941 gagccacatc aggttgagcg caatattcac aaccgtatcc gcccgataag ccagcgacat
    15001 ttggatgttc atttttagaa atgcagataa aagcttgagt gtttgcatgt tttatgctcc
    15061 cacggctgaa aaacgcttga cgccattgcg ccagacccag ttgaaaagaa gaatggagac
    15121 cgccagccac acgcctgcca tgatgtatcc ctgaacgatc tggttggtgt tgagctttcc
    15181 gagaataagt tggacgggaa aataaagcaa caactgaaac ggcaaatatt gggcgatatc
    15241 ctggatcacc tttggcatga gtggaagcgg cacaaattga cccgagaaaa gcaggatgag
    15301 ggcaaagtag aactcgtgaa tggagtacac gcgtgtcgtc caaaatgcga gggaggtgat
    15361 ggtggcagaa agcaggtacc cgatcagaaa agaaaccgcc atgactggaa tggacagcaa
    15421 aattccctgc agagtgacgc tgctgaagtc gggcttgaaa atcagaaaca gaatggccca
    15481 tacggggatc aggacgatga cattcaagat cttgtttgca atattgacca ccagactgtt
    15541 ggtcagaaca gggtggattg gcttggtcaa ttcacctgaa agtgtgccgt cctgaatttt
    15601 gtaggcgaag atgtgaatga agatgttgct ggtgagctgg tcgcagatga tgatcagcat
    15661 gtagtaggtg atgaagtcgt tggccgtaaa gccgcttaca tctccctttg cattggcaat
    15721 gctcgtccat acggcgaggt aaataatggg ggagaccagc cagtaaagca ggtacatcac
    15781 aaggttggcg cggtattgcc actgctccgc ccagtttacc tgccagagac gtttgaagat
    15841 gagtaacatg gattttcctt tcttcattgc acgatgcgcc cggaaggcgc tcgtcaggtt
    15901 gaagccttcc tgcaatgaca gatggtctac tgattgaatg cctgctcgat cacatcctca
    15961 atgggcggat cggcgatggt aatgtcgtgt atcggcagat ctgccaacag cctggaagtg
    16021 acccgcgtca catcctcagc cttgacctgg atgtactgct tctcggcatc ttcttcgttt
    16081 tgaacgggtt cgccatattt tgaaaggtcg tgcgaattgg attctgccag caataccccg
    16141 atcaatttga atggggcgat ctttttggag aggtcattga tgccgccatc atatttgagg
    16201 ctgccatgat gaatgatgat gatgcgctca caaagcgcgg tcacatctgc catgtaatga
    16261 ctggtcagca aaatggttgc gcctgtgcgt ttgttgtact cacgcagaaa ctcgcggatg
    16321 cgtgcctgcc ccgtgatgtc gagaccgatg gtgggttcat ccagaaatag aattttcgga
    16381 cggtggagca gccctgcggc gatctcgcat ttcatgcgtt cgccaagcga aaggttgcgc
    16441 acgggtttct tcatcaacgg ctcaagttca agcagttcat ccagttcctt gtatgtcttt
    16501 ttgtattcat cgtcagggat tcggtaaatg gcctgattga gcaggaacga atccgccgcg
    16561 gggatatccc acgaaaggcg gttgcgttga cccatcacaa gggtcatcac ccgcagatat
    16621 tcaggcttga gttcccaggg ggtgaacccc agcacttcgg cttttccacc agtggggtgc
    16681 agtaaaccag aaagcatctt gagcgtggtg gtctttcccg cgccgttcgg gccaaggaaa
    16741 ccgacgatct ctccctgctc gatcttgaaa ttcacctgtt gaaccgcttt tacatccttg
    16801 tatttgcgtt tgaaaaaact acggactgcc gcgccaaaac caccttcgcg ctcaggcact
    16861 tgataggtct tgctaagttg ttggacgttg atcgttgggg acataaaaat ccttcctttg
    16921 tggggaggaa ggattttatc attagtagaa caaaagagct agtcttgaag ttctgaaacc
    16981 cgataccaaa tgtccacgcg catgtgcagg gttttcgtgt gttggatgtc cattcccaag
    17041 ccttgttcgg cgggggctgg cgctccacga aacacgggag ccgattgcgc ttgaaaccgc
    17101 ggtgcttcat cgtccatcgg tgtttcaaga aaatcataaa ccccgatcaa tttcactctc
    17161 agggtatccg ccacctgttg ggcctttttc tttgcatttt caagcgcgcg attcagcagt
    17221 tgtcggcggg tttcttcgtc atcgtatcgc cattcgattc gttcgagcgt ggcgttcttt
    17281 tgcgcggaga tgccatcgag gatcgcggac agtttttcca gatcttcaca gcggatcttg
    17341 agtcgataca ccgcgctgga tgactttaac agagcgccgc ctgcctgttc aagatggaca
    17401 ccctgaagga agaacgcttc gctcttaagc ccgaggctgg tcagtgcttc aacaagctga
    17461 ttcacctcct tggctttttt catcgcttcg ctcccgctga ccagcgatgt gcctttgatg
    17521 gtcacgtgca aatctgcatg tgtggcggag atttcctctt tctccatgat ggagatcttg
    17581 atggtgtcgg gtttggtggt catttttctc ctttgcgttt caaagaaggt gtttctcttt
    17641 gtgcctgtgt taagttacgg tcaatttttg cttcgaatat ttttcgtggc gcttttcttc
    17701 catcacacgt ttgatccgct caaagccttc ccatacatcg gtgaatgagg tgtacagggg
    17761 tgcaaaaccg aggcggatgt tatcgggttc gcggaaatct gggatgacgt tcatttcttc
    17821 gatgagggcg cggttgattc gataaccgtc tgcgtggcgg atgctgatgt gcgatccgcg
    17881 tttggcaggg tcaggcggag agccgagcga aaagccgaag ggggcgagcc agctttcggt
    17941 taagaaagag gcgtagcctg tcatcaaaat ggatttttcg cggatggagt ccatgcctgc
    18001 ttgcagggtg ggttcaaggg cggcttccat ggtaagcagg gaaaggatgg gctgtgtgcc
    18061 gaccagaaaa cgttctgcgc cgcgggcggg ttggtaattg aggctgaaat cgaacggatc
    18121 gttttgcccc caccagcccc agatgggcga ggatagtttt tcctgaatgg atttgttgac
    18181 gtagagaaat gcgggtgcgc cagggccgcc gttgagatat ttgtaggtgc agccgatggc
    18241 aagatcggca ttgcaggcat caaggtgaat gggcagcgca ccgacggagt gactcaggtc
    18301 ccaaaggacg agggcgcctt ttttatgggc gagttctgta atgcgctgca tgtcgtagag
    18361 atagccgctt ttgaaggtga catgcgaaag tgtgaccagc gctgtgtttt cgtcaattgc
    18421 agtttcaagc gccgcaaggt cgggggtgat gtcgttgtcg cttgcgccga tggagatgat
    18481 ttcgtggcgg ccgccaaagg tctgcctgat gccctgcaaa atatacaggt cagatgggaa
    18541 attgaaagtg tcggtgatga tgcgggtctt gctgggctgc aaggtgagcg cggcggcggc
    18601 gagtttgaac aaattgagtg agaccgtgtc gttgaccaaa acctgccctg ctgctgcgcc
    18661 gatgagttga ccgatcttgt cgcccacccg cgcaggagaa tcccaccagc ctttgttcca
    18721 cccgcggatc aaatccacgc cccattgttc ttccacgact tggcgggctt tggcctgtgc
    18781 cgctttgggc agcaggccga gtgagttgcc gtcgagatag atcaggtttg ggtcgtggat
    18841 gacgaattcg ttgcgaatgg cggccagcct gtcgtgggta tccaattgct gggcgaattc
    18901 gcgcgcggtg gaaaaactca tggacattta tttcttttca gccgataaaa acttgaacgg
    18961 atacgaagtg tagtaaaccg accagtcttc gccttctttg atgagctctt gcaggctttc
    19021 cttcacttcg aattttttat cgcccagatt caagtgcagc acttttacat cttcataact
    19081 gcggacactg ctgcccggtg tgctgacgcg ttttgtgccc caagtgtatt ccactttgcc
    19141 tcttgcggtt gtgacggtta tttcggcggg tttgctggcg cgcatgacgt aaatagccac
    19201 cacagcccca acggttaaca ctgcaatacc ggtaaaaaca atgccgaaac cacccatggc
    19261 agccaggggc agaatgtcct gcaagtctga gagatcgccg ttgagcagca tgggcaatac
    19321 cactctcgac ccgatcaaca gcgctggcag gcataagaac aggaaggcga ccacgccgcc
    19381 caccccaaga aacacactgc gctgcgattt gtgttcgccg acaagatatt tcttctgctt
    19441 ttcggttaat tgccccagac ggttcgcacc gaggtcggct tcgtcaaact tccaatattc
    19501 catgatcgat tcaggcattt aaaagagatc tcctcaaaaa ttgttttggc ttgttcagtc
    19561 agtggttgcg ctgcggggcg gcaccttgct catggcgtat tcggccaatg cggtaattgt
    19621 cagcgaaggg ttcacgcccg ggttggcagg catgatcgaa ccatcaatga tgtataagcc
    19681 gggataatta tgcacagcga aattttcatt aacaaccccc tcttcggggg attttccgat
    19741 cggtgcgccg ccaaggatgt gcgcggtggt gggcaggccc aacagatttt caccgatcga
    19801 gccgagcgcc acaccattcg tccgtttggc gaattcgcgt gtgatctcgt gtgagccttt
    19861 cacctgcgcg ttgatcttga acccaggttc ttcatcagcg accagaccag tgcggaaaag
    19921 ggtgaacagg ctgcgtccca ttcgaaagcg cattcgattg tcggcgtttt gcatcaccag
    19981 caaaatggtc acgttgtgcg cccagcccgg cagaaaagct gcttttgcaa aatcaatggg
    20041 gtgggtgagc gcccagccta aaaattttat gatccgcatg gggatgctgg cgttgttgtc
    20101 gatcaacggt gcggcgagga aacgcatcaa cgacgagcca tcggggtagc gcactggttc
    20161 gatgcgggtg atctcatcat ggttgtagat ggatgaaatg gatacgcctt ccgagtagtt
    20221 gatatcggat ctgcgtgcca cgcttcccaa caagccctct gagttggtcc tgaccattgt
    20281 gccgagtctc gccgaaagtt tgggcagtga tttcttcacg tcgcgcagat tcagcagcaa
    20341 tttcatggtc cccatcacac ctgcggagaa gatcacattt ctggcgtgga cgatgtgttg
    20401 cgatttgagc agggaagtgg aggggcgata atgcacttcg tatcgggcac cagtttccag
    20461 cgcagttgga agcggtttta catccatcac ttcgagctcg gctttgactt gcgcgccttt
    20521 tttctcggcg aaatacaaat aatttttcgg cagggtgttt ttggcgttgt ggcggcatcc
    20581 caccatgcat ccaccgcagt gctggcaccc agcgcggttg ggaccttctc cgccgaagaa
    20641 tggatcgggc acggtaacgc ctgcctcacc aaaaaacgcg ccgacatctg tggcgcggaa
    20701 ggtgtgcccc atgccgcgtt cttctgcaaa gtctttcaac agcaggtcgg ctttccataa
    20761 tttggggttg cgtgctgcgc caagcatttt cttcgccgtt tcatagtgcg gacgcaggac
    20821 atcgccccat ttgatgtttt gattccaggc gggggtggcg aaggtttctt cgttgggtac
    20881 ttcgagcaca ttggcgtagc ccaaacttcc gccgcccaca cctgcgccgt gcagcaccat
    20941 cacacctttg aggatggcga tctgcaaaat gccatgcgca cgcagggcgg gaatccagac
    21001 atatttccaa tattgccagt tggaggttgc gaaatcttta tcgttgaagc gtttgccttt
    21061 ttcgagaacg aggaccgaat accctttttc ggccaatcgc attgctgaaa cacttccccc
    21121 gaaccccgag ccaatgatca cataatcgta gatttgagac attcctgcat tctccgcgtt
    21181 gagttgacgt gattatacgt catgacgcgg aggcgtggat gagtcagcgc ggcaggtgga
    21241 aaacgatcat catgccgtct tcggtctctt cgaccgattc tgtatttccg ccgtgcaatt
    21301 tgatcagcga ggagctgatg aaacccagca tggctgagtc catttcgaac ttgtcgcgtg
    21361 attttcggcc agtgctttga acggtgatga ccgcgccgtt gtcgttgtcg cgggcggaga
    21421 gggagagcga cccttcgacg acgcgggtgg cggcgaggct gagcaggctg gtgacgatct
    21481 ggttgacgcg cgccaggtca atgttgtaaa cgggttcgtg gatgatgata tcggttgtga
    21541 ggtttttgtt gggattcttg acctgccact ggtccgctgt gttgacgagg atgctgcgca
    21601 attcgcaggg ttcgcggttg agggtgatct cgccgttgtt gaggcgggcc atatccacca
    21661 ggttgccgac cagcgccagc atgcgttgac cgccgttgta gaccgtggtg aggtcggtgg
    21721 tttggatgtc gttgacgggg ccgtccatgc ctttcatcac cacacgggtg aagccgatga
    21781 tggcgttgag cggggttttc aggtcgtgcg aggcccagga gaggaattcg tccatatcga
    21841 agcggcctgt gctgcgattg ttccacgcca tttcttcgat gagctttggg ttgcctgcca
    21901 gtacgccagc ctgttcgtcg ttgagtttgt gcaattgata aaggcggatg gccagttccg
    21961 tttttgcctc gtcggctgtc agttttgccg cttccaaaag gtcggttggg atttcaagga
    22021 tggttttatc catgacggtc tcgtttcgag tgctgaaaat gataaaggta ttcgtagtat
    22081 agccgattag acagcaaaaa caagccgttt ttcagcgggg a
//
LOCUS       DAOQLC010000072        21558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_835_length_21558_cov_23.238571, whole genome shotgun sequence.
ACCESSION   DAOQLC010000072 DAOQLC010000000
VERSION     DAOQLC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_835_length_21558_cov_23.238571"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..241
                     /locus_tag="PL000_13185"
     CDS             23..241
                     /locus_tag="PL000_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HNC89902.1"
                     /translation="MRRMFGFLIGILVGALVGSTVALLMAPESGEKLRGELRDRGQGF
                     MNDVRHAANSRRIELRGRLESLRAPRAE"
     gene            complement(287..1459)
                     /locus_tag="PL000_13190"
     CDS             complement(287..1459)
                     /locus_tag="PL000_13190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC89903.1"
                     /translation="MKILFVADGRSPISRNWIRYFAERGDEVYLASTFACELDFPVKR
                     LELTPVAFSAVKKQTSTPGSGSSRTLGLRTRIRQWLGPVTISRSARKLRAFIQEVQPD
                     IVHALRVPYEGMLAAEALSGRRVSRPRFLVSIWGNDFTLHAPSTPLMGYYTRKVMKAA
                     DALHADCERDIRLARAWGLGADQPTLVAPGNGGIRGDVFYPPSELVKEPVIINPRGFR
                     AYVKNEAFFKSIPLVLAKRSDARFVCSSMQGEAQALKWIQELGIEHAVQLNPPLSHAQ
                     MGEVFRGAQIVVSPSIHDGTPNSLIEGMACGCFPVAGDLESIREWITPGQNGLLVDPN
                     NPQAIADAILIALEREDLRREAAGLNANMISAKAEYGRTMERVVEFYEMAIQGNRE"
     assembly_gap    1481..1580
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1719..2108
                     /locus_tag="PL000_13195"
     CDS             1719..2108
                     /locus_tag="PL000_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89904.1"
                     /translation="MSKGSVLIVEDNMDTYELVNFILQKDGFDTFLAVNGRDGVDAAI
                     KQKPDLIIMDLSMPEMDGWAATSLIKKNPTVKSTPVIVLTAHALPGDRQRAMDAGCDQ
                     YITKPMDLGELVEVIHYWIKKAKSSQP"
     gene            2133..3428
                     /locus_tag="PL000_13200"
     CDS             2133..3428
                     /locus_tag="PL000_13200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNC89905.1"
                     /translation="MKQICVVGVGYVGLVTAACFADLGNRVTALDVNEKRVENLKKGI
                     MPIYEPGLDELVKRNVNAGRITFTTSYKEALQGAEYAFIAVGTPSGEKGEADLQYVAA
                     AAKSIAENMTAPIVIINKSTVPIGTGDWVADIVKGAQPKPMQFAVVSCPEFLREGSAI
                     GDFMNPHRTVIGSLDKDAANKVAHLHLPLRAPIVITDLRTAEMIKYASNAFLATKISF
                     INELADLCELVGADVKEVAAGMGYDARIGRHFLDAGLGWGGSCFPKDVEALAFMAKEK
                     GLNPRILNDVMEVNYDRRTTAVKHVEKMIGSELKGKTIGLLGLAFKPNTDDMRDAPSI
                     DIADALLKAGAKVRAYDPVAMEVARPILPAVDMFDEPYKMAKDCDALMVVTEWNEFKQ
                     LDLEKLKGLLKSPIIYDGRNIYDPRLMKEMGFTYRAIGR"
     gene            3567..4469
                     /locus_tag="PL000_13205"
     CDS             3567..4469
                     /locus_tag="PL000_13205"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC89906.1"
                     /translation="MTNPPVCNYEGSDYQQTFWDQGGREYEDRAEAIALKRMLPTSGK
                     LMLELGAGAGRNTPRYAGFERIVLLDYSTTQLAQAQQRLGRSAQYIYVAADVYRLPFR
                     QHQFDAATMIRTLHHMADAPKALGQVRNVLRSNAVFILEFANKLNLKSILRYWLGRQD
                     WNPFSLEPVEFVKLNFDFHPKAVRNWLGGLGFSIEKTLTVSHFRLGFLKRVIPAKLLA
                     AFDGLFQPTGALWQFTPSVFLKAKVNENTQNAEIPLSVLGLFKCPECSGENLQEKNDH
                     LLCPNCNARWSVNDGIYDFREKMK"
     gene            4503..5246
                     /locus_tag="PL000_13210"
     CDS             4503..5246
                     /locus_tag="PL000_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89907.1"
                     /translation="MTTDPQKPLPHVQPFVERKTQPPRKNGVDTRGMLSVVTMLVSLA
                     ALTVSMLGAVKLVFDIFDDGLSGSKLEGLLVKLTVLGFAFLFGWAVGLVSIRAFGNLV
                     YPIIVKVYSWGCLIGTSVLYINVIQKLYFQKYDGLHFWAYLIMLLSGLFVLICLHLLV
                     EGHDLRPYAIPLLIISVLQLFVIVIRYVFDENPNGLMLFGDFTIFIVMISISALMLMH
                     IGILSPLRDQINNLFMRNRPSVKNGNGAG"
     gene            complement(5315..8521)
                     /locus_tag="PL000_13215"
     CDS             complement(5315..8521)
                     /locus_tag="PL000_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNC89908.1"
                     /translation="MTLPNQSASTRKTIAVLGAQLSRSWGAEFMDGVLESARASDVNV
                     VCFVGGRPMAIASPQHGGHSYGLYDLIKPGQFDGILLSADLGHGASSEALGDFCRVFE
                     PTPVVSFAIPAEGVTSFSVDNEGGMRSMIRHLIEVHGYKRIAFIRGIQGHLEADQRYN
                     AYLEELKAHDIRFDEQLVVDGDYTNESGRAAARVLLDDRAVRVQAIVASNDRMAFGAI
                     EVLQQRGIQVPDSIAVTGFDDVNESRSMGVPLTTVNQSFFETGKKTFDALMKRMNGEK
                     LSDVNVIPTQLVVRWSCGCLPENVKRAVVLPREVSHTGRLENKRDAAIRALFGAAGIP
                     EDSPERVQYVDVFGRTWDMFLVSLREVEKSDTFLKMVQSMVELLQRQGYDSTTWHNVI
                     SIFRRYALGGITSNTTMLRAENLFQQARMLVGELSQRAQAFRRLQFEQQEEALSNFSF
                     SMAPAMTLEEIGDALSRHFPILGLNRWYLMFYSDFASPGSISSPTPESYRLLLQYDQN
                     KFQIPQEKSSIATGRLVPRGKTPEDHRYAAIVMPLALASNRFGFMWAEMGPTDWDVYM
                     RLKNLLSSALLRTMLVQQREQAQQEVERLLSEARERAVELARARDAAEKAAAQNAKLF
                     ESEQIRRRGAEALARSSRQLSSLTTIEKLPQQILEQLKQVLPYDRGILLMEDVNGVPR
                     IRAHHGMPANQDLSDFQIQLDGKDFYKTIARKGETLLVHNVEAVKDWRQPAWLPRDHS
                     WMGVPLYSKDKVIGLLMLSRMYSGFNEDDGLLATTFAMQAIVALENARLYDEVTGINQ
                     MMERMVSQRVEELNNAYNTLAKHDKNKSAFIQVAAHELRTPLTVIKGYLGMLSVDAVV
                     QANPVLAQAIEGVSQGTSRLHQVVSSMLDVARLDNQITNPHVEPVSLNLILRLIHKDY
                     VKDLETRHLNFELDEAIKDIPPLLADPELLKKALDHVVVNAIKFTPDGGTISISAIVF
                     EDARLGKQAEIRIKDTGIGIDPEHHKIIFEKLFQLGTVELHSSSRTNFKGGGAGLGLA
                     IASGIVKALQGNIWVESVGHDETNFPGSTFVIRLPLVK"
     gene            complement(8604..8927)
                     /locus_tag="PL000_13220"
     CDS             complement(8604..8927)
                     /locus_tag="PL000_13220"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC89909.1"
                     /translation="MSTAPKSQILTLAEMASYQDAAVVSRQITKAEAGNVTLFAFDKD
                     QGLSEHTAPFDALVHILEGEALVTISGKPFELKAGDAIIMPANEPHALQAIQRFKMLL
                     TMIRA"
     gene            complement(8982..11021)
                     /locus_tag="PL000_13225"
     CDS             complement(8982..11021)
                     /locus_tag="PL000_13225"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HNC89910.1"
                     /translation="MIDNIDTEKRSGISAVAIGIVALLCCICVLVGGMVWYGYYAFTQ
                     AIPAISTFEPPTNDDPFFPVDPTEPVIEPELTRPPVDSINGETLQLLETTVVPPNEPK
                     ELACRMEGKCDIPDVMATTAAPRVVGDTDSFWVTDVGTNENTEVQATLRYVTPHVYFW
                     VQDGVSYDDGEMKALMDAFENKIYPTNREFFGSEWSPGIDGDEHIYILYARGLGSSIA
                     GYFSSADSVHPLVHEFSNAHEMFLFNADNTFLGEEFTYGVLAHEFQHMIHWNQDLNET
                     SWLNEGSSELAAFLNDYDPGGFDWLYITDPDMQLNDWPNDQDATTPHYGAGFLFMTYF
                     LDRFGEDATKALIKDPANGLDSVENALREIDATDPVTGQPISADAFFMDWAVTNFLLD
                     KSVGDGRYIYNNYPAANRASATETIYNCPQTPIKRDVHQYGVDYIAIECAGDHTISFT
                     GSTVTGLLPADPYSGDYAFWSNKGDESDMTLTREFDFTNVSGPIELSYQTWYDIETDW
                     DYLYLETSEDGVTWEIVTTPSGTDTDPSGNSYGWGYTGVTNGWIEEKVDLSQFAGKKV
                     FVRFEYVTDAAVNGEGLLLDDVKVEAAGYSSDFETDDGGWEAKGFVRVQNVLPQTFGL
                     SLILTSDSSVTMIPLNPDQTAEISISLQSGQKAYLVVSGTTRFTRELASYQIEIK"
     gene            complement(11202..13676)
                     /locus_tag="PL000_13230"
     CDS             complement(11202..13676)
                     /locus_tag="PL000_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC89911.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVIPQILEKLSVSPEALTERLDATLRASPKANIFGGGAGQIFITPRVKRIIDLANEEA
                     NRLKDEYISTEHIFLAILTERNTPAARILESAGLTRDRVYDAIQDLRGGQRVTDPQAE
                     TRYRTLEKYSRDLTQLAREGKLDPVIGRDKEILRLIQILSRRTKNNPVLIGEAGVGKT
                     AIAEGLAQKIASNDVPEILSGKKVVALDLGAMIAGSRFRGEFEERLKAVMDEVQRAKG
                     DVILMIDELHTVVGAGAAQGAMDASNMLKPALARGELQCIGATTLDEFHKHIEKDAAL
                     ERRFAPIYVDEPSVEDTIKMLHGLRDRYEAHHKIHFSDDALTAAARLADRYVTDRHLP
                     DKAIDLMDEAAAKLRVALYSMPPDLKALKGEVDRLHAEEEQAGLERDYERAAQKKAER
                     LRLEQEYTVKRDKWEADHQLDEVVDVDDIASVVHQWTGIPVNQMLETESEKLLHMEAR
                     LHERIIGQDEAIRAISDAIRRARSGLKDPARPIGSFIFIGPSGVGKTELAKALAWFMF
                     DDEEALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVVLFDEIEK
                     AHPEVWNALLQILDDGRLTDGQGKVVDFRNTVLIMTSNLGTEYVTKGGTLGFLTQKAD
                     VDERAMHDKIEKALKGAFRPEFLNRVDETIMFSPLSIEQMENIVVLQMKEVQDRLNEH
                     NITVQLTDAARGWLAKEGFDPAFGARPLRRAIQKYVESPLSVELLGGKFKDGAMVMVD
                     VDAENNKIIFHTEVAPAKASKQRVEA"
     gene            14194..16923
                     /locus_tag="PL000_13235"
     CDS             14194..16923
                     /locus_tag="PL000_13235"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89912.1"
                     /translation="MDNLIGKHVDRYEILELLGTGGMAMVYKAYDARLEREVAIKIIR
                     RSAFPPEVIDDMVKRFEREAKSLAKLSHANIVKVLDYGEFEGSPYLVMEYYPGGTLKE
                     KMGEPVEWQDAARIILPVARGVEYAHDRGIIHRDIKPANILMNEKGDPTLSDFGIAKL
                     LQGESKNNLTASGAAVGTPEYMAPEQWTGETSTKTDMYALGTILYEMITGRRPYVADT
                     PGAVFLKQVTEPLPLPRKFNANLPEMVEEFLLKTLDKDPANRYEDFQAFIKELETLLG
                     DDTLSSLEKASPKTTAEKGTQEKTSAQKTDRESPSKQENAPRKTFPLYAGIGGGVLLV
                     ILIAFFALRNNPTADTPSTETSAPSVDATVTATTAPVSNTEPTTGPQKLDIGSVIISE
                     IDGMNLLYVPAGEFTMGNYADDALIDCEKGKSEALNEYNCSRELFLDEEPPTPVNLDA
                     FWIDETEVTNEMYAECVAESVCAQPADISSSTNASYYNNPAFKHFPVIYVDWNMAKTY
                     CEWAGRRLPTEAEWEKAARGTDSRVYPWGFAPPNSNLLSYSEGVYIRDTVEVAESPDG
                     RSIYGAYDMAGNVYEWTNSAYEKYPYDPTDGREEFKAIDDLRIMRGGSWRSNNVDIRS
                     ANRYPALPTDTYNSGGIRCARSEQPGDALIPPTPIPTEIGKRNTENYDIGSTITGEDG
                     ATFVYVPAGKFIMGSDDGKADEQPVQELYLDAYWIGETEVTNKQYEACVNQGTCDPPA
                     SFASYNQKGYFGYPQYINYPVLYVSWDQAKTYCTWAGGRLPTEAEWEKAARGTEGQTY
                     PWGNATPNSNLLNYNSDLFDTSEVKKYPDGQSIYGAYDMAGNVWEWVSSIYRPYPYNS
                     LDGREDMTLEAERVLRGGSWLVSDADVRASNRYYSSQKETFNNLTGFRCARSE"
     gene            17097..18500
                     /locus_tag="PL000_13240"
     CDS             17097..18500
                     /locus_tag="PL000_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89913.1"
                     /translation="MKKKLLAALLTCVLVSCSPNVTFTPQATVAQIPPTETLSPLPTL
                     TPNPVTIIDPTTIPSPAPIPVSTATEIYSLDNLRMAYIVDGNLYIQNGSNPPTQLTDN
                     RQDIYPMFSDDGEKIVFYRGRNNENNSIFSINADGSHIQEIITKGWLDTLGVGTKAGH
                     LAFIPNTHQFLFNTYLCPDNDDMYSGCTVGLFLADTNTGKLKEIVKPALGGRLLLDGD
                     SRWGGNFSISPDGNLLSIAHAGQIDILSMDGKVIHHNIMEYPPGMPIELYPRVYWFPD
                     SSGFITALPAEKEYYFWVMSGNPAYTIWRYTFDKNIATQIPLDPTLLWIRMDSNDVVS
                     VSPNREWVVYFANDCQLYKGNLLDGSTELLLPCKYHLEIQWNLDNMHFASGAYPEESI
                     FGSIDSPLGYPPGYFLGWIDAKRFLYFPASARSKENIQLLVGEIDGEIILSYESNVFI
                     PNKAPYPPIFFVYSFGE"
     gene            18625..19485
                     /locus_tag="PL000_13245"
     CDS             18625..19485
                     /locus_tag="PL000_13245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNC89914.1"
                     /translation="MESTNPQPIPETESKPPVPEQEVREAPQAKPAVRKPRAVNAFAT
                     TLGVAVLLATLFTAWTPNSLFASNLQDQLNLILTPQAGQNPVITSPQPQLRIGIVAGH
                     NGNDSGAVCYDENGEVDLKEADVNFEIATLVQKSLEAQGYQVDLLNEFDTRLNGYRAV
                     ALVSIHNDSCEYINDEATGFKVASSLETRDVNRAQRLNSCLVDRYERITGMIFHANSI
                     TGDMREYHAFSEIDPNTITAIIETGFLNLDREILTERTDLVAEGVTQGILCFMNNESV
                     LPTAIPTFVP"
     gene            19482..20237
                     /locus_tag="PL000_13250"
     CDS             19482..20237
                     /locus_tag="PL000_13250"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC89915.1"
                     /translation="MKNPPALPRRSFLIPISIFILIVLVTLAAAWTATNSPAFGLTTP
                     PTPTLRTQSFAFAQIAKPTYTVTAAPTDLPPASTATAESAPTAESTATTQAMVVAEIV
                     EDTAVIDYPAPASQPMEISAPGYGGNKYILVDISEQHMYVYEGDALVYSFVSSTGMNN
                     ATRVGSFSVLDKIPNAYGATWDIWMPNWLGIYWAGSLENGIHALPILSNGATLWSGYL
                     GIPISYGCVVLGSYESQLLYDWADVGTPVDIQW"
     gene            20364..21338
                     /locus_tag="PL000_13255"
     CDS             20364..21338
                     /locus_tag="PL000_13255"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC89916.1"
                     /translation="MNNRLPTNNSNGRPSPEKQNQAKVQKKKRGPLLPILLVLMGCAV
                     FAFAAWSAINSPVLASLLNSAPAPQQAQPVQRPFAQVSISKPSAVVVQQPLIQVAMPT
                     ATLPALPVENTAQQPQPQVIDPAPTADLALNAAALPTLQVSVLPGATDAPAVEQQPAA
                     EQAPVVQEANSQPGVISAEIVVDTPTPEYVAPTEVAAAPVPVGVDTGGEHWIDVDLSQ
                     QRVYAYAGDTVVNSFVVSTGTWQTPTVTGKYKVWVKLRSAAMSGPGYYLPDVPYIMYF
                     YGSYGLHGTYWHNNFGTPMSHGCVNLTIPDAEWLYNFSSVGTVVNVHY"
BASE COUNT         5289 a         5990 c         5378 g         4801 t
ORIGIN      
        1 aaccaagtga aggagaattc acatgcgaag aatgttcggt tttctgatcg gaattttggt
       61 gggggcgttg gtggggtcca ctgtggcatt gctcatggcg cctgaatcgg gcgagaaact
      121 gcgcggtgaa ctgcgcgacc gcggacaagg cttcatgaac gacgtgcgcc acgcggcaaa
      181 ttcacgccgc atcgaactgc gcggacggtt ggaatcgctg cgagcaccgc gggcagaata
      241 aggaatagat aatggggaat ggggaatagg cagcctccac cattcactat tccctattcc
      301 cttgtattgc catttcgtaa aactccacca cccgctccat tgtccgccca tactctgctt
      361 tggcagagat catgtttgca ttgagacctg ccgcctcgcg ccgcaggtct tctctttcca
      421 gcgcgatcag aatcgcatcg gcaatcgcct gcggattgtt cgggtcaacg agcagaccgt
      481 tttgcccggg cgtgatccac tcgcggatgg attccagatc acccgccacg gggaaacatc
      541 cgcaagccat accctcgatc aacgaattcg gcgtgccatc gtggatactc ggcgaaacca
      601 cgatctgcgc cccgcgaaag acctccccca tctgcgcgtg cgaaagcggt gggttcaact
      661 gcaccgcatg ttcgatgccc aactcctgaa tccatttcaa cgcctgcgcc tcgccctgca
      721 tagacgaaca cacaaaacgc gcatcgctcc gtttcgccag caccagcgga atcgacttga
      781 aaaacgcctc atttttgaca tacgcccgaa atccgcgcgg attgatgatg acaggctctt
      841 tcacaagttc acttggcgga taaaaaacat cgcctctgat tccgccgtta ccgggcgcca
      901 ccaacgtggg ttgatcagcg cccaagcccc atgctctcgc gaggcggata tcccgctcac
      961 aatcggcgtg gagcgcatcc gcggccttca tcaccttgcg cgtgtaatat cccatcaacg
     1021 gtgtggacgg tgcgtgcagg gtaaagtcat tgccccaaat ggaaacaaga aagcgcggcc
     1081 tcgatactcg acggccagat agcgcctcag ccgccagcat cccttcatac ggcacacgca
     1141 gcgcgtggac aatatctggc tgaacctctt gaatgaacgc acgcaatttt cgcgcggagc
     1201 gggaaatggt cacggggccg agccactgtc ggatacgcgt acgcaggccg agggtccgcg
     1261 aagaaccgct gcccggggtc gaagtctgct ttttgacggc gctaaaggct acgggggtca
     1321 attccagccg cttgaccggg aaatccaatt cacaagcaaa agtggaggcg agatacacct
     1381 cgtcaccgcg ctccgcaaag taacggatcc agtttcgaga gatcggagag cggccatcgg
     1441 ccacaaagag gattttcata ggtgaaagcg attataccca nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn gagagcggcc atcggccaca aagaggattt tcataggtga
     1621 aagcgattat acccactgta gagcgggata ataatcccgc tcagttcgtt tagaaaccgt
     1681 acattgcccg taaacgcgag acgtagtata tttgcggcat gagcaaaggt agtgttttga
     1741 tcgttgaaga caatatggat acctatgaat tggtgaattt catcctgcaa aaggatgggt
     1801 tcgatacttt tctggcggta aacgggcgtg acggtgtcga tgcggcgatc aaacaaaaac
     1861 ctgacttgat catcatggac ctttccatgc ctgaaatgga cggttgggcg gcgaccagcc
     1921 tgatcaagaa aaaccccaca gtgaaatcca ctccagtcat tgtattaacc gcacatgcgc
     1981 tgcccggcga ccgccagcgc gcaatggatg cgggctgcga ccagtacatc accaaaccga
     2041 tggatctggg cgaacttgtg gaagtgatac attactggat caagaaagcc aaatcgtcac
     2101 aaccctaatc tttttttcaa agaggaacaa acatgaaaca gatctgtgtc gtcggcgtcg
     2161 gatacgtcgg ccttgtcact gccgcttgtt ttgccgatct cggaaaccga gtcaccgcat
     2221 tggatgtaaa cgagaagcgc gttgaaaacc tcaaaaaggg catcatgccc atttatgagc
     2281 ccggtctcga tgaattggta aaacgcaatg tcaacgcggg ccgcatcacc ttcaccactt
     2341 cctacaagga agccctgcaa ggcgccgaat atgccttcat tgcagtaggc accccctccg
     2401 gcgaaaaagg cgaagcggac ctgcaatacg tagccgccgc agccaaatcc atcgcggaaa
     2461 atatgaccgc gccgatcgtc atcatcaaca aatccacggt tccgatcggg accggtgact
     2521 gggtggccga cattgtgaag ggtgcccagc ccaagccgat gcaatttgcg gtcgtctcct
     2581 gccccgaatt tctgcgcgaa ggttccgcca ttggcgattt tatgaatccg caccgcaccg
     2641 tgatcggctc gctggataag gatgccgcca acaaggtggc ccacctgcat ttgcccctgc
     2701 gcgctcccat cgtcatcacc gacctgcgca ccgcagaaat gatcaagtac gccagcaatg
     2761 ccttccttgc cacaaagatt tccttcatca acgaactggc tgacctgtgc gaactggtcg
     2821 gcgcggatgt gaaggaagtg gccgccggta tgggctacga tgcgcgcatc ggccgtcact
     2881 ttttggatgc aggcctcggc tggggcggtt cgtgtttccc caaggacgtg gaggcgctcg
     2941 ccttcatggc caaggaaaag ggactcaatc cgcgcattct caacgatgtg atggaagtga
     3001 actacgaccg ccgcaccacc gccgtcaagc atgtggaaaa gatgatcggc agcgaattga
     3061 aaggcaagac catcggcctg ctcggtttgg cgttcaagcc caacaccgat gacatgcgcg
     3121 atgccccctc catcgacatt gccgacgccc tgctcaaggc gggcgccaaa gtccgcgcgt
     3181 atgaccccgt ggcaatggag gtggcccgtc ccattttgcc cgctgtagat atgttcgatg
     3241 aaccctacaa aatggcaaaa gactgcgatg cgctcatggt cgtcaccgaa tggaatgaat
     3301 tcaaacagct tgacctcgaa aaattgaagg gattgctcaa atcccccatc atctacgatg
     3361 gacgcaacat ctacgatcca cgtttgatga aggaaatggg cttcacctac cgcgccattg
     3421 ggcgataatt tcagcaatct gatcgttcgc tctccgaata gtcggatggt ctgcaaaggc
     3481 cattcgacta ttttgcctat ttgaccaaaa gctaaaaaaa cactacacta caaaaaacaa
     3541 tcatcctcca accacaagac caaaacatga ccaatccccc cgtttgtaac tacgaaggct
     3601 ccgattatca acagactttt tgggaccagg gcgggcgcga gtatgaagac cgcgccgaag
     3661 ccattgccct gaaaagaatg ctccccacca gtggaaagct catgctcgaa cttggcgcag
     3721 gtgcaggccg caacacaccg cgttatgcag gcttcgagcg cattgtcctg ctggattact
     3781 ccaccaccca actggcacag gcccagcagc ggctgggacg ctcggcccaa tacatttatg
     3841 tcgccgccga tgtgtaccga ttgccattcc gccagcatca attcgatgcc gccaccatga
     3901 tccgcaccct gcaccacatg gcagatgccc ccaaagcgct cggtcaggtc aggaacgttc
     3961 tgcggtcaaa cgccgtcttt atccttgaat ttgcgaacaa actcaacctg aaatcgatcc
     4021 tgcgctattg gctgggtcgg caggattgga acccgttcag ccttgaacct gtggaattcg
     4081 tgaagttgaa tttcgatttc catcccaagg cggtccgcaa ttggctgggc ggactcggct
     4141 tttccatcga aaaaacactg accgtctcac atttccgcct cggatttttg aagcgtgtga
     4201 ttcctgccaa acttctggcc gcgttcgatg gtttgttcca gcccacgggt gcgctctggc
     4261 aattcacgcc cagcgtcttc ctaaaggcca aagtgaatga aaatacacaa aacgccgaaa
     4321 ttcctttgag tgtgttgggt ttattcaaat gccccgaatg ttcaggcgaa aacctgcagg
     4381 agaaaaatga tcacctgctc tgcccaaact gcaatgcgcg ctggtcagtg aacgacggca
     4441 tttacgattt ccgtgaaaaa atgaaatagc accacgaatc ctgcaaggcc gggagatgcg
     4501 ccatgacaac ggaccctcaa aaaccactgc cccacgttca accgttcgtg gaaagaaaga
     4561 cacaacctcc ccgaaaaaac ggagtggata cgcgcggcat gttgagcgtg gtcaccatgc
     4621 tggtcagcct cgccgcgttg accgtttcga tgctgggggc ggtcaaactg gtctttgata
     4681 tttttgatga tggcctgagc ggcagtaaat tggaaggcct gctggtaaaa ctgaccgtgc
     4741 tggggtttgc gttcttattt ggctgggcag tggggctggt cagcatccgt gctttcggga
     4801 atctggttta cccgatcatc gtcaaggttt attcctgggg atgcctcatc ggcaccagcg
     4861 ttttgtacat aaatgtcatt caaaagctgt attttcaaaa atatgatgga ctgcatttct
     4921 gggcatattt gatcatgctg ctgagcggct tgtttgtgct gatctgcctg cacctgcttg
     4981 tggaaggcca tgacctgcgc ccttacgcca ttccactgct gatcatcagc gtgctgcaat
     5041 tattcgtgat cgtgatccgc tatgtatttg atgaaaatcc aaacgggttg atgttgttcg
     5101 gcgattttac gatcttcatt gtgatgatct cgatctcagc cttgatgctt atgcatattg
     5161 gcatcctctc gccgctgcgc gaccagatca acaacctgtt catgaggaac cgtccctcgg
     5221 tgaaaaacgg gaacggggca gggtaaacca gccaaaaata aaatcacccg cgaaatttga
     5281 tttcgcgggt gattttattt ttacagtgtt agtgttactt caccaatgga agccgaatga
     5341 cgaaggtgct gccggggaag ttcgtttcgt catggccgac actttcaacc cagatatttc
     5401 cctgcagggc tttcacaata ccggaagcga tcgccaaccc cagcccggcg ccgccaccct
     5461 tgaaattggt gcggctggag gagtgaagtt ccacggtgcc aagctggaac agtttttcaa
     5521 aaatgatctt gtggtgctct ggatcaatgc cgatgcctgt gtcttttatg cggatctccg
     5581 cctgtttgcc caggcgggca tcctcaaaca caatggcaga aatggagatc gttccgccat
     5641 cgggggtgaa tttgatcgca ttgacaacga catggtcaag cgccttcttg agcaattcag
     5701 ggtcagccaa aaggggcgga atatctttta tcgcttcatc aagctcaaag ttaagatgac
     5761 gcgtttccag gtccttcaca taatccttgt ggatgagtcg caaaatcagg ttgaggctga
     5821 ccggttccac atgcggattc gtgatctgat tatccagacg ggccacgtca agcatgctgc
     5881 tgacgacctg atgcaggcgg cttgtgcctt gtgaaacgcc ttcgatggcc tgtgccaata
     5941 ccgggtttgc ctgtacaacc gcatccacac ttaacatgcc gaggtagcct ttgatgaccg
     6001 tgagcggtgt acgcaattca tgtgcggcca cctgaatgaa ggcggacttg ttcttgtcat
     6061 gtttggccaa cgtgttgtag gcattattca actcttcaac acgctgtgag accatgcgtt
     6121 ccatcatttg attgatgcca gtcacttcgt catacagacg cgcattttcc aatgccacga
     6181 tggcctgcat ggcaaaggtg gttgccagca atccatcgtc ttcattgaat ccgctgtaca
     6241 tgcggctgag catcagcaaa ccgatcacct tgtcttttga ataaagcgga acacccatcc
     6301 acgaatggtc gcgtggcagc caggcaggct ggcgccagtc tttcaccgct tccacattgt
     6361 ggacaagcag ggtttcgcct ttgcgggcaa tggttttgta gaaatccttg ccatcaagtt
     6421 gaatttgaaa atcagaaaga tcctgatttg cgggcatgcc atgatgtgcg cgaatacgcg
     6481 gcacaccgtt aacatcttcc atgagcagga tgccgcggtc atagggcagg acttgtttca
     6541 actgctccag gatctgttgg ggaagttttt cgatggtggt aagcgatgac aattgacgtg
     6601 acgaacgcgc caacgcttct gctccgcgac ggcggatctg ctcggactcg aacagttttg
     6661 cattttgcgc cgcggctttt tcggcggcat cacgagcgcg ggcaagttcc actgcacgtt
     6721 cacgcgcctc actcaacaag cgttccactt cctgttgggc ctgttcgcgc tgctgtacga
     6781 gcatggtccg caaaagggcg cttgaaagca ggttcttcaa ccgcatgtaa acgtcccaat
     6841 cggtgggacc catctcagcc cacatgaaac cgaagcgatt acttgccaac gccaaaggca
     6901 tgacaatggc cgcgtaacga tggtcttcgg gcgtctttcc acgcggaacc agccgtcccg
     6961 tagcgatgga ggatttttcc tgtgggatct ggaatttatt ctggtcgtat tgcaaaagca
     7021 aacgataact ttcgggggtt ggagaagaga tcgagcccgg ggaggcaaaa tcgctgtaga
     7081 acatcaaata ccagcggttc aaacccagaa tcgggaaatg ccgcgaaagc gcatcaccga
     7141 tctcttccag ggtcatggcg ggagccattg aaaagctgaa attgcttaag gcctcttcct
     7201 gctgttcaaa ctgtaaacgg cggaaggcct gcgcccgctg cgacaactca cccaccaaca
     7261 tacgcgcctg ctgaaagaga ttttcagccc gcagcatcgt ggtgttgctt gtaattccac
     7321 cgagtgcgta ccgtctgaaa atggaaatga cgttgtgcca ggtcgttgaa tcgtaaccct
     7381 gacgctgcaa caattcgacc atggattgca ccattttcag gaatgtgtcg ctcttttcca
     7441 cttcacgcaa gctgacgagg aacatatccc aggtgcggcc aaacacatcc acatattgaa
     7501 cccgttcggg gctgtcttcg gggatgcccg ccgcaccaaa caaagctcgg atggcggcat
     7561 cacgcttgtt ttcgaggcgc cccgtatgtg aaacttctct tggaagcacc accgcccttt
     7621 tcacgttttc aggcagacaa ccgcatgacc aacgcaccac caattgggtg gggatgacgt
     7681 ttacgtcact tagtttttcg ccattcatgc gtttcatcaa cgcatcaaag gtctttttcc
     7741 ctgtttcaaa aaatgactgg ttgaccgtgg tcagcggcac ccccatggac ctcgattcat
     7801 tgacatcatc gaagcctgtc acggcgatgc tatcaggcac ctgaatgccg cgttgctgca
     7861 atacttcgat ggcgccgaac gccatgcgat cattggaggc cacaatggcc tgcacacgga
     7921 ccgcacggtc atccaaaaga acgcgcgcag cggcgcgtcc actttcgttg gtatagtcgc
     7981 catccaccac cagttgctcg tcgaagcgga tgtcgtgcgc ttttaattct tccaaatagg
     8041 cgttataacg ctggtcggct tccaaatgcc cctgaattcc ccgaataaag gcgatccgtt
     8101 tataaccatg aacttctatc aaatggcgga tcatggaacg cattccgcct tcattatcca
     8161 ctgaaaaaga ggtgacccct tcggcaggga tcgcgaacga aaccaccggg gtgggttcaa
     8221 acacgcgaca aaaatcccca agagcttcag aagaagcacc atgaccaagg tcagccgaaa
     8281 gcaaaatacc gtcgaactgc cccggtttga tcaaatcgta caacccgtag gaatgcccgc
     8341 catgttgggg cgaagcaatg gccattggcc tgccccccac aaaacaaacg acatttacgt
     8401 cgctggcacg cgccgactcc aggacgccat ccatgaactc agcgccccaa ctacggctta
     8461 actgcgcgcc gaggacggcg attgtttttc gtgtactggc agactggttt gggagtgtca
     8521 ttttgagtgg caaaattata cgtcaggaaa tatgaatcgt gtctgtcaat tttgaaaaga
     8581 aatgaatttg tcacacagtg cttttatgcg cggatcattg tcaacagcat tttaaaacgt
     8641 tgaatggcct gtaaggcgtg cggttcattg gcaggcatga tgatggcatc tcccgctttc
     8701 aattcgaacg gctttcctga aatggtcaca agcgcttccc cttcaaggat gtgaaccagg
     8761 gcatcaaacg gcgctgtgtg ttcgctcaag ccttgatctt tatcaaaggc aaacaaggtg
     8821 acattgccag cctccgcttt ggtgatctgg cggctgacca cggcggcatc ctgatacgat
     8881 gccatttccg cgagggtcaa aatttgtgat ttgggtgcag tagacatgag tactccaaaa
     8941 aaagcaggtc acacccgtgg gtgtgacctg ccgattattt attattttat ttcgatctgg
     9001 taggaggcca gttctctcgt gaaccgtgtg gtgcctgaga caaccagata tgccttttgc
     9061 ccggactgca aggaaatgga gatctcagca gtttgatcgg ggttgagcgg gatcatcgtc
     9121 acgcttgaat cgctggtcaa gatcagagac aggccaaagg tttggggaag aacgttttgg
     9181 actcggacaa agcccttcgc ttcccagccg ccgtcatccg tttcaaaatc ggaactgtag
     9241 cctgctgcct caactttgac atcatccagc aataggcctt caccattcac cgcggcatct
     9301 gtcacatatt caaagcggac aaacaccttc ttgccagcga attgggaaag gtccactttt
     9361 tcttcgatcc agccgttggt tacaccggtg tagccccagc catacgaatt cccggagggg
     9421 tcggtatcgg tgcccgaggg tgtggtaacg atctcccagg tgacgccatc ttccgaggtt
     9481 tcgaggtaga ggtaatccca atcggtttcg atatcgtacc aggtctggta ggaaagctcg
     9541 atggggccgc tgacattggt gaaatcgaac tcacgcgtca gggtcatgtc tgattcatcg
     9601 cccttgttcg accagaaggc gtaatcaccg gaataaggat cggcgggcag caacccggtg
     9661 accgttgaac ctgtgaacga aatggtgtga tcaccggcac attcaatggc aatgtaatcc
     9721 acgccatatt gatgcacgtc gcgtttgatc ggtgtctgcg ggcagttgta gatcgtctca
     9781 gtggcagaag cgcggtttgc cgcaggataa ttgttgtaaa tgtaacggcc atcgcccacc
     9841 gatttatcaa gcaggaagtt ggttacggcc caatccatga agaaggcatc ggcgctgatg
     9901 ggctgaccgg taacagggtc ggtggcgtcg atctcacgca gggcattctc cacgctgtcg
     9961 aggccatttg ccgggtcttt gatgagtgcc ttggtggcat cctcgccaaa acgatccagg
    10021 aagtaggtca tgaataaaaa gcctgcgcca taatgcgggg tagtggcatc ctgatcgttg
    10081 ggccagtcgt tcaattgcat atcagggtcg gtgatataca accagtcgaa tccaccgggg
    10141 tcgtaatcgt tgaggaatgc ggccagttcc gaagaaccct cgttcaacca ggaggtctca
    10201 ttcaggtctt gattccagtg gatcatgtgt tggaattcat gcgccagcac gccataggta
    10261 aattcctcac cgaggaaggt gttatcggca ttaaagagga acatctcatg agcgttggaa
    10321 aattcatgca ccaacggatg cacagaatcg gcggaggaga aataacctgc aatggaagaa
    10381 cccaatccgc gggcatagag aatgtaaatg tgctcatcgc cgtcaatgcc aggcgaccat
    10441 tcactgccga agaattcgcg gttggtgggg tagatcttgt tttcgaaggc atccatcaac
    10501 gccttcattt cgccgtcatc atacgacaca ccatcctgca cccagaaata cacatgcggg
    10561 gtgacgtatc gcagggtggc ctgcacctcg gtgttttcat tggttcccac atcagtgacc
    10621 cagaacgaat cggtatcacc aacaacacgc ggagcggcgg tagtggccat cacatcgggg
    10681 atgtcgcatt tcccttccat gcggcaggcc aattccttcg gctcattcgg cgggacaact
    10741 gttgtttcca aaagctgcag tgtctccccg ttgatggaat ccactggcgg gcgggtaagc
    10801 tctggttcaa tgacgggttc agtggggtcc accgggaaaa atggatcgtc attcgttggc
    10861 ggttcaaacg tggaaatggc gggtatggcc tgggtaaagg cgtaatatcc ataccaaacc
    10921 ataccgccaa ccagcacgca gatacagcac agtaaagcaa caatgccaat cgcaacggct
    10981 gatattcctg atctcttctc tgtatcaata ttatcaatca tggtcatcct ctggttggtt
    11041 aagatgcccc atgataccgt gagtgcgtga aaaagggatt aggaaattgg agggggaagg
    11101 aaataaaagg atgaaggatg aaggatgaag gatgaaggat gaaaaaaaga cgaaagaagc
    11161 atttgctttc tttcgtcttt ttactttcta ctttctactc tttacgcctc aacccgctgt
    11221 ttggatgctt tcgcaggagc tacttcggtg tgaaagatga tcttgttgtt ttcagcatcc
    11281 acatccacca tcaccatggc gccatctttg aacttgccgc cgagcagttc caccgaaagc
    11341 ggactctcga catatttctg gatcgcgcgt cgcagcggac gggcgccaaa ggcaggatca
    11401 aagccttcct tggcaagcca gccgcgcgcg gcatcggtca actggacagt gatgttgtgc
    11461 tcgttcaggc gatcctgcac ttctttcatt tgcaggacaa cgatattttc catctgctca
    11521 atcgagagcg gcgagaacat gatggtctca tccacacggt tgaggaactc ggggcggaat
    11581 gcgcctttga gcgccttttc gatcttatcg tgcatggcgc gttcgtccac gtcggctttt
    11641 tgggtgagga agcccaaagt gccacccttg gtcacgtatt cagtgcccag gttcgaggtc
    11701 atgattaaga ccgtgttgcg gaaatccaca accttgccct gaccatccgt caagcggcca
    11761 tcgtccaaaa tttgaagcaa ggcattccac acttcagggt gcgccttttc gatctcatcg
    11821 aagaggacga cgcgatacgg gcggcgacgg acggcttctg taagttgacc gccttcttca
    11881 taaccgacat atccgggagg cgcgccaaag aggcggctga ccgtgtgctg ctcgcggtat
    11941 tcagacatat caatgcgaac gagcgcttct tcatcatcga acatgaacca ggcaagagcc
    12001 ttggcaagct cggtcttccc cacaccagac ggaccgatga agatgaacga tccgatcggg
    12061 cgggcgggat ctttcaaccc agaacgcgcg cggcgaatgg catcagaaat ggcgcgaatg
    12121 gcttcatcct gaccgatgat gcgttcatgc aaacgcgctt ccatatgtaa caatttttct
    12181 gattcggttt cgagcatctg attcacaggg atgcccgtcc actgatgcac gacggaggcg
    12241 atgtcgtcca catccaccac ttcatcaagt tggtgatcgg cttcccattt atcgcgcttc
    12301 actgtgtatt cctgttcgag gcgcagacgt tcggctttct tttgcgcggc gcgctcgtaa
    12361 tcgcgttcga ggccagcttg ttcctcttct gcgtgcagac gatccacttc acctttcaag
    12421 gctttgagat cgggcggcat ggaatagagc gccacacgca atttcgcggc ggcctcatcc
    12481 atgaggtcaa tcgctttatc gggcaggtga cggtcggtca catagcggtc tgccagacgg
    12541 gcggcggcag tgagagcatc atctgaaaaa tggatcttgt ggtgcgcttc gtaacggtca
    12601 cgcaagccgt gcagcatctt gatcgtatct tcaacgcttg gctcgtccac atagatcgga
    12661 gcgaaacggc gttcgagtgc ggcatctttt tcgatgtgtt tgtggaattc atccaacgtg
    12721 gtcgcgccga tgcattgaag ttcgccgcgg gccagtgcgg gcttgagcat gttgctcgca
    12781 tccatcgcgc cctgggctgc tcctgcaccg acgaccgtat gcagctcgtc gatcatcagg
    12841 atcacatcgc ctttggcacg ctgtacttcg tccatcacgg ctttgaggcg ttcttcaaat
    12901 tcaccgcgga agcgtgaccc cgcgatcatt gcaccgagat caagcgcaac aaccttcttg
    12961 cccgaaagaa tttcaggcac atcgttgctg gcgattttct gtgcaaggcc ttctgcgatg
    13021 gcggttttgc ccacacccgc ttcaccgatc agaacgggat tgttcttggt gcggcgggac
    13081 agaatctgaa tgaggcgcaa aatttccttg tcacgcccaa tgaccgggtc cagttttcct
    13141 tcgcgggcga gttgggtcag gtcacgtgaa tatttttcca gggtgcggta acgcgtctcg
    13201 gcttgcggat cggtgacgcg ctgtccaccg cgcaaatcct gaatggcatc ataaacacgg
    13261 tcacgggtca ggccagccga ttccaaaatg cgggcggcgg gcgtgttgcg ctcggtgaga
    13321 atggcaagga agatgtgttc ggtggagatg tactcatctt tcaggcggtt ggcttcctcg
    13381 ttggccagat caatgatccg tttgacgcga ggcgtgataa aaatctgccc tgccccaccg
    13441 ccaaagatat tggccttcgg gctggcacgc agggtggcat ccaaccgctc ggttaatgcc
    13501 tcggggctga cgctcaattt ttcaaggatc tggggaatca ccccgccggg ctgttcgatc
    13561 aacgccagca ggatgtgttc ggtatcaatt tgattatggc cgtagcgctg aatgatctcc
    13621 gcggcgcgct gggcggcttc ctgagctctc tctgtaaatc ggtcaaatct catcatggga
    13681 tagttcctcc gttggctgac gggtaaaaag acgatagaca acggacggtt tagcggcagt
    13741 cagtggtcaa tggtctgcga tcagtcaatg ggggcattat aacaaaagca ggatttgcag
    13801 attgtatata aggcgtaaga ggtgtgtaag atttttgccc ccgttgggaa cagtgaagcc
    13861 agacttttag atatggcagt agcaaagtcg cttgacaaaa atagagactt ggaacgcgaa
    13921 tagtgcgaat gggcgaattt cgcaaatttt cttggattta ttcgccccat tcgtaaattc
    13981 gtggcattcg tgttgaggtt ttcctgactt tgctatagcc atggattttt cgacgcaatt
    14041 actcccattt gcgagagacc taatgtataa tcccgaccat ccttgtgggg aatccacttc
    14101 ccaataccaa accattcctt cacccttcct caaaagacaa aattcatcca cctctccatc
    14161 aacaacatca tatctcctga aaaggcagcg accatggaca atctgattgg aaaacatgtc
    14221 gaccgatacg aaatcctcga actcttgggc acaggcggca tggccatggt atacaaagcc
    14281 tacgacgccc gcctcgagcg tgaggtggcg atcaagatca tccgtcgcag cgcctttcca
    14341 cccgaggtga tcgatgacat ggtcaaacgg tttgaacggg aagccaagtc gctggcgaaa
    14401 ttgtcacatg ccaacattgt gaaagtgctc gattatgggg aattcgaagg ctcgccgtat
    14461 ctggtgatgg aatactaccc cggcggaaca ctcaaagaaa agatgggcga acctgtcgag
    14521 tggcaggacg cggcgcgcat catcctgccg gttgcacggg gggtcgaata tgcccacgac
    14581 cgcggcatca tccatcgcga tatcaaacct gcgaacattt tgatgaacga aaaaggagac
    14641 cccaccctct ccgattttgg aatcgccaaa ctgcttcaag gcgaatcgaa aaacaacctg
    14701 accgcctccg gcgcagccgt cggcacaccg gaatacatgg ccccggaaca atggacgggc
    14761 gaaaccagca ccaaaacaga tatgtacgcc ctcggcacca ttctctatga aatgatcacc
    14821 gggcgcaggc cgtacgttgc cgataccccg ggcgcggtct tcctcaaaca ggtcaccgaa
    14881 ccgctgcccc tcccaagaaa attcaacgcc aacctgccag agatggtgga agagtttctg
    14941 ctcaagaccc tcgacaaaga cccagccaac cgttatgaag attttcaggc cttcatcaaa
    15001 gaactcgaaa ccctgttggg agacgacacc ctcagcagtt tagaaaaggc cagccccaaa
    15061 accacggctg aaaagggaac ccaggaaaaa acatccgcac aaaaaacgga ccgtgaatca
    15121 ccttcaaaac aagaaaacgc cccgcgcaaa actttccccc tttatgctgg gatcggaggc
    15181 ggggtgcttc ttgtgatttt gatcgcgttc ttcgcgcttc gcaacaaccc gacggctgat
    15241 acaccgtcga cagaaacctc cgccccgtcg gtggatgcga ccgtcaccgc aaccacggcc
    15301 cctgtttcca acacagaacc cactacgggc ccacaaaaac ttgacattgg ctccgtcatc
    15361 atttctgaaa tagatggcat gaacctgctt tacgttccag caggagaatt caccatgggc
    15421 aactacgccg atgacgcctt aatcgattgc gaaaaaggca agtctgaagc gctcaatgaa
    15481 tacaactgtt cgcgcgaatt atttttagat gaagaaccgc ccactccggt caacctcgat
    15541 gcattttgga tcgatgaaac ggaagtcacc aacgaaatgt acgcggaatg tgtcgccgaa
    15601 agtgtctgcg cccaacctgc agatatcagt tcatccacca atgccagcta ttacaacaac
    15661 ccggccttca aacatttccc agtcatttat gtggattgga acatggcaaa gacctactgc
    15721 gaatgggccg ggcgcaggct ccccaccgaa gccgaatggg aaaaagccgc ccgtggcaca
    15781 gacagccgcg tctacccctg gggctttgca ccccccaaca gcaacctgtt gagttacagc
    15841 gaaggcgtct acatccgcga tacagtggaa gttgctgaaa gccccgatgg ccgaagtatt
    15901 tacggcgcct acgacatggc aggcaatgtt tatgaatgga caaacagtgc ctacgaaaaa
    15961 tacccctacg accccaccga tggccgcgaa gaattcaaag ccatagacga tctgcgtata
    16021 atgcgcggcg gatcgtggcg cagcaacaac gtcgatattc gcagcgccaa ccgctacccc
    16081 gcccttccca ctgacaccta caacagcgga ggaatccgct gtgcccgcag cgaacaaccc
    16141 ggggacgcgc tcatcccacc cacgcccatc cccactgaaa ttggaaaacg caacactgaa
    16201 aattacgaca tcggctccac catcacgggt gaagacggcg ccacctttgt gtatgtgcca
    16261 gcgggcaaat tcatcatggg cagtgacgac ggcaaggcag acgaacagcc cgttcaggaa
    16321 ctttatttgg atgcctactg gatcggcgaa accgaagtga ccaacaaaca atatgaagcc
    16381 tgtgtcaatc agggcacctg tgacccgccc gcttcatttg catcctacaa tcaaaaggga
    16441 tatttcggct accctcaata cattaattat cccgttctct atgtaagctg ggaccaggcc
    16501 aagacctact gcacctgggc tggcggcagg ctccccaccg aagcggaatg ggaaaaagcc
    16561 gcccgcggca cggaaggcca aacctacccg tggggcaacg ccacccccaa tagcaacctg
    16621 ctgaactaca actcagacct ttttgacaca agcgaagtga aaaaataccc cgacggccaa
    16681 agcatttacg gcgcctacga catggcaggc aacgtctggg aatgggtcag cagtatttac
    16741 agaccatacc catacaacag cctcgatggc cgcgaagata tgaccctcga agccgaacgc
    16801 gtcctgcgcg gcggatcgtg gctcgtcagc gatgccgatg tacgcgcctc caatcgctac
    16861 tatagcagtc agaaagaaac attcaacaac ctcaccggct tccgctgcgc ccgctctgaa
    16921 taaaccatca gccgaaaata aaacgaatca agaatcctcc gagttttgtg tgctgaaaaa
    16981 ctcggaggat tcttattttg tgcgataagc agccaggaag gaatgatcag ataacgctat
    17041 aatttatatt ctggtggggg aactgaaacg acacacctcc ccaaaaagga taccttatga
    17101 aaaaaaaact actcgccgcc ctgttgacct gcgtactggt cagttgctcg cctaatgtga
    17161 ctttcacccc gcaggcgact gtggcacaaa tccctcccac agagaccttg tccccgctgc
    17221 cgacgcttac ccccaatcca gtcacaatca ttgatccaac aacaatccca tctcctgccc
    17281 cgataccagt atccacagcg actgaaatct attcgctgga taatctccgc atggcatata
    17341 ttgtggatgg aaatttgtat attcaaaatg gcagtaaccc acccacacaa ctaacggata
    17401 acagacaaga tatttatccc atgttctccg atgatggcga gaagattgtc ttttatcgtg
    17461 gcaggaataa tgaaaataac agtatatttt cgatcaacgc cgatggaagt catatacaag
    17521 aaatcatcac aaaaggttgg ttggatactc tcggcgtagg cacaaaagcg ggacatctgg
    17581 cgtttatccc caacacgcat caattccttt ttaatacata cttgtgtcct gataatgacg
    17641 atatgtattc aggctgtaca gtgggactat tccttgcgga taccaatacg ggaaaactca
    17701 aagagattgt gaagcccgcc cttggcggtc gtttgctttt ggatggcgat tcgcggtggg
    17761 gaggcaattt cagtatatcc cctgacggga atttattatc catagctcat gcaggacaaa
    17821 tcgatatact cagcatggat ggcaaggtta ttcaccacaa catcatggaa tatcctcctg
    17881 gaatgccaat tgaactttac ccaagagtgt actggtttcc agattcaagc gggtttatca
    17941 ccgccctccc agcagaaaaa gaatattatt tttgggtgat gtctggaaat cccgcctaca
    18001 ccatttggag atacacattc gataaaaaca tagcaacgca aatacctttg gatccaactc
    18061 tgttatggat acgcatggat tctaatgatg tagtgagcgt ctcaccaaac agggaatggg
    18121 ttgtttactt tgcaaatgac tgccaactat ataaaggtaa tcttttagat ggaagtacag
    18181 aattactcct accatgcaaa tatcatttgg aaattcagtg gaatttggac aatatgcatt
    18241 ttgctagtgg tgcatatcca gaggaatcta tttttggctc tattgactct ccgctaggtt
    18301 atcctccagg atattttttg ggttggattg acgcaaaacg atttctttac ttccccgcct
    18361 cggcaagaag taaagaaaat atacaacttc tcgtaggtga aattgacggg gaaataattt
    18421 tgtcttacga gtcaaatgtt tttattccaa ataaagcgcc atacccccct atcttttttg
    18481 tttactcttt tggagaataa ataaaagaga tggtttcccc cgctcaagcc gccgtccccg
    18541 gcgaagcgag caaacctgcg tacaaatttt aggccaagcc tttttctcac tccccgtttt
    18601 tgcggtaaga ttacgtcata gaacatggaa tctacaaacc cacagcccat tcctgaaact
    18661 gaatcaaaac cacccgtacc cgaacaagag gtgcgcgagg ccccacaggc caagcctgcc
    18721 gtccgcaagc cgcgcgcggt gaatgccttt gccaccacac tgggagtcgc tgttttactg
    18781 gcaaccctct tcaccgcatg gacccccaac agcctgttcg caagcaatct gcaagaccaa
    18841 ctcaacctca tcctcactcc gcaggcgggg cagaaccccg tcatcacttc gccccagccg
    18901 cagttaagaa tcggaatcgt cgcaggtcac aatggcaacg actcgggcgc agtctgctac
    18961 gacgaaaacg gcgaagtgga tctgaaagaa gccgacgtca attttgagat cgccaccctc
    19021 gtacaaaaaa gcctcgaagc acagggatat caggtggacc tgctcaacga attcgatacg
    19081 cgcctcaatg gctaccgtgc cgtggcgctc gtttccatcc acaacgactc ctgcgaatac
    19141 atcaatgatg aagcgacagg cttcaaagtg gcatcctcgc ttgaaacgcg cgatgtcaac
    19201 cgcgcccaac gcctcaactc ctgccttgta gaccgatacg aacgcatcac cggcatgatc
    19261 ttccacgcca acagcatcac aggcgacatg cgcgaatatc acgccttctc cgaaattgac
    19321 cccaacacca tcaccgccat catcgaaaca ggcttcctca acctcgaccg cgaaattctc
    19381 accgaacgaa ccgaccttgt tgccgaaggg gtgacacaag gcatcctctg cttcatgaac
    19441 aacgaaagcg tcctgcccac cgccatcccc acttttgtgc catgaaaaat ccccccgccc
    19501 tcccgcgacg cagttttctg atacccattt cgatcttcat cctgatcgtc ctcgtcaccc
    19561 tcgccgcagc atggacggca accaactccc cagccttcgg cctgacgacg ccccccaccc
    19621 caaccctccg cacacaatcc tttgccttcg cacagatcgc aaaacccacc tacactgtca
    19681 ccgccgcccc caccgattta ccgcccgcat caaccgccac agcagaatcc gcaccgactg
    19741 ccgaatccac agcgacgact caagccatgg tcgtggcaga gatcgtggaa gatacagcgg
    19801 tcatcgacta ccccgcgcct gcctcacaac cgatggaaat ctccgccccg ggatatggtg
    19861 ggaacaaata catcctcgtc gacatctcag aacagcacat gtacgtctat gaaggcgacg
    19921 cgctggtgta cagctttgtc tcatccacag gcatgaacaa cgccacccgc gtcggctcct
    19981 tcagcgtgct cgataaaatt cccaacgcct acggcgccac ctgggatatc tggatgccca
    20041 actggctcgg catttactgg gcgggcagcc tcgaaaacgg aatccacgcc ctgccgatcc
    20101 tctcgaacgg cgcaaccctg tggtctggct accttggcat ccccatctcc tatggctgtg
    20161 tggtgctcgg cagctacgaa tcgcagctcc tctacgactg ggccgatgtc ggcacccctg
    20221 tggatattca atggtaaaaa acgaatcaca gcaaacgtcc aaggcgtata attccatcac
    20281 cactgcctac tgcttactga ttaccgatta ctaattactg attactgact accgattact
    20341 gactaccgaa aggcatctcc accatgaaca accgcttacc caccaacaac tcaaatggca
    20401 gacccagccc ggaaaaacag aatcaggcca aagtccagaa aaagaagcgc ggacctttgc
    20461 tccccatcct gcttgtttta atgggttgcg cggtcttcgc ctttgcggcc tggtcagcga
    20521 tcaactcacc ggtgctggca tccctgttga acagcgcccc cgccccgcaa caggcccaac
    20581 ccgtacagcg gcctttcgcg caggtgagca tttccaaacc ttctgccgtt gtggtgcaac
    20641 agccattgat tcaggtagcc atgcccaccg ccaccctgcc ggccctgcct gtggaaaaca
    20701 cagcccagca gccccaacca caggtcattg accccgcccc cacagcagac ctggccctca
    20761 acgcggccgc cctgcccaca ctgcaagtct ctgtgctgcc aggcgccacc gatgccccag
    20821 ccgtcgagca gcaacccgcg gctgaacaag ctcccgtggt tcaagaagcg aacagtcagc
    20881 ccggggtcat ctctgccgag atcgtggtgg atacccccac acccgagtac gttgccccaa
    20941 ccgaagtggc ggcggctccc gtccccgtcg gtgtggatac cggcggcgaa cactggatcg
    21001 atgtggacct ttcgcagcag cgtgtgtatg cctatgcagg tgacaccgtg gtcaattcct
    21061 tcgtggtttc gacaggcacc tggcaaaccc ccactgtgac aggtaaatac aaggtctggg
    21121 tcaaattgcg ctctgcggcc atgtctggcc ccgggtacta cctgcccgat gtgccctaca
    21181 tcatgtattt ctatggcagt tatggtttgc atggcacata ctggcataac aatttcggca
    21241 cacccatgag tcatggctgt gtcaacctca ccatccccga tgccgagtgg ttgtataact
    21301 tctcttcagt ggggactgtg gtgaatgtgc attattaacc cccaaattcc caaaaacgga
    21361 tttttgggat gcgaaggccg ccccaaagat ttaaccgctt ttgaattttc ttcgcgttct
    21421 ttgcggcctt cgcggttaga aaaggttcct gcgtggactc atttataaat tccttgtaaa
    21481 aaattcaaag aaaatgaagt catccaaatt atcgcgacgt gattttctga aactgggcgg
    21541 attgggtctt gctggctt
//
LOCUS       DAOQLC010000073        21188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_863_length_21188_cov_22.142810, whole genome shotgun sequence.
ACCESSION   DAOQLC010000073 DAOQLC010000000
VERSION     DAOQLC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_21188_cov_22.142810"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PL000_13260"
     CDS             <1..269
                     /locus_tag="PL000_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89917.1"
                     /translation="VTPEMLPRAMRWMFPIFKLLVRDDGGKSAAKAARSSVYLASSPE
                     VEGVTGKYFDTNCKEAAWSPAVMDDDMRKTLWEQVEQITKRQSG"
     gene            complement(297..1787)
                     /locus_tag="PL000_13265"
     CDS             complement(297..1787)
                     /locus_tag="PL000_13265"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC89918.1"
                     /translation="MADQKHREIFSQGRKVWNAWRKKNPKIKPDLSTLSFRNADLGGF
                     DLRAVFLENSDLRNAQCVGTDLRGAYLYNVDARKANFDKANLSGATLQRALFQGASLR
                     NAKIEFTLSQEANFEKADLTNASLFLSDLSDAKFDQAILKNANFNETNLNGTYLTQCD
                     LSSCTFKKASLVNVKMVNSNLTGVDFSGRTLAGASFWRANLTRANIKETDCQMVEFAE
                     AKLVGANLSFSNLDTAEFRDADLTSADLHGSYLANTSLLRAVVEKANFKNCYVYGTSA
                     WDLIGTPKSQQDLIITPPSEPAITVDDLEVAQFVYLVYNNKKIRNFMNAFTDKNVLIL
                     GRFSLPERKAVLDGLREKLRTFDFVPIVFDFDSPQDKDYTETVQTLAGMSMFVIVDVT
                     NPKSTPLEMEATVKQFKIPYLPIIDTSADDRPFAMMIDLQKSFHWVLPTFGYKTRKEL
                     LNNLKAAIIDRAIEKHDQLRDQKAKESISMLTVDDLKKRSGAKRKK"
     gene            complement(1870..2502)
                     /locus_tag="PL000_13270"
     CDS             complement(1870..2502)
                     /locus_tag="PL000_13270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC89919.1"
                     /translation="MPLLLLIRHGENEYVKTGKMAGRLPGVHLNERGQKQAQALGDAL
                     KDVPIKAIYSSPLERAMETAAPIASARNLKIIQEPDLMDTDIGKWQGKSWRVLGLTKA
                     WKIVQNAPSRFRFPEGESFPEAQLRIANVLERVVQTHKKPQDIVAVVFHADPIKLAVS
                     HFIGMPLDHFQRLACDTGSMTVLHVGEAGAHLVKLNQRPPFDFLPKENKK"
     gene            complement(2517..2966)
                     /locus_tag="PL000_13275"
     CDS             complement(2517..2966)
                     /locus_tag="PL000_13275"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNC89920.1"
                     /translation="MDEIRPWLFIGGFRDTLDKGYLDRKLIRSMLQIAEPIQHEGINS
                     LFLQVDDLARTPHHLIKQGVDFVLAEKEKGHNILVSCGAGVNRSTMFCLAALKEAEGL
                     SLFDAFKEVSQRHPGALPNEQVWEAFCDYYHESVPYLDVMRLAAKNF"
     gene            complement(2968..4374)
                     /locus_tag="PL000_13280"
     CDS             complement(2968..4374)
                     /locus_tag="PL000_13280"
                     /inference="COORDINATES: protein motif:HMM:NF009883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC89921.1"
                     /translation="MSDLFDHAMQERMKTEAPLAARMRPRTLDEYIGQEHIVGEGKLL
                     QRAIKADKLFSSIILWGPPGTGKTTLAQVIANSTKSHFVTISAILAGKADLRVVIDEA
                     LERRRLHNERTILFVDEVHRWNKAQQDALLPHVENGTFTLIGATTENPYFEVIKALIS
                     RSRVFQLRNLNQTETGVLLDRALSDKERGYGNKRITLDLDARSHLIEVASGDARNALN
                     ALELAVESTTPDRDGIIHITLDVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDP
                     DAALYWLAKMIYAGEDPKFIIRRLIILAGEDIGLADPMGLVVASSAAQAFDYIGLPEG
                     IYPIVEATLYLATAPKSNSAGAYFSAMKKIEEEGQVSVPRHLMDGNRDAAAMGHGKDY
                     VYPHDQEGHFTPQQYLPRRLLGTYFYSPSQEGYESQVTARLEMWREAQRKALNIERTE
                     HVPSLSEEQIVEMKRKIK"
     gene            complement(4378..4791)
                     /locus_tag="PL000_13285"
     CDS             complement(4378..4791)
                     /locus_tag="PL000_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNC89922.1"
                     /translation="MKRAVLDTNILISSALGGALVLVLEKWDAGVFSVVVTTDILSEY
                     FEVLNRPKFGLKQETIDKITTYIYQFSEFVIPEEKIRFIEDDPKDDKFLEAAIAGNVD
                     FIVSGDKHLLDLKEFRSVPILSGREFLDWLQRNGN"
     gene            complement(4788..5021)
                     /locus_tag="PL000_13290"
     CDS             complement(4788..5021)
                     /locus_tag="PL000_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89923.1"
                     /translation="MTTFEQTLLREVATLPQSRQADVLAFIRFLKISLPESEKARSDF
                     KEALEDARATAKKLNITQEDIEAEIRAVRDGKQ"
     gene            5121..6398
                     /locus_tag="PL000_13295"
     CDS             5121..6398
                     /locus_tag="PL000_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HNC89924.1"
                     /translation="MKKSPTEISEAVEAAREELIAFLQKLVQTASLPNHEHDVQNLVA
                     QKLRSMGMEVEIIPSNFDNLKGHPAFGDDGFSPTDRVNVIGRWPGTRTGEGKSIILNG
                     HVDVVPVGDLSLWVDSPWSGVIRDGKMYGRGSCDMKSGLCAGIFAVDVLQKLGYQPAN
                     DILIESVIGEESGGIGTLTTIVKGYTADAVILLEPTRLELSPVQSGALTFRLTVPGRS
                     IHAAMKAYGVSAIEKFALLLNVINKLDAERHASFKHPLFEDPHNIAPINIGTVRGGEW
                     HSTVPEEVVAEGRMGVFPGESNEAARKMFEAAIQTAAESDPWLSQNPPRVEWFEGQFE
                     SGETPLEHPLIQNLAAMHEQVTGSAATLRGVTYGSDMRLFTNHAKIAATHYGPGDVGM
                     AHAVNEFVPLDEVVTAAKVVANTVAQWCGGEFK"
     gene            6395..7273
                     /locus_tag="PL000_13300"
     CDS             6395..7273
                     /locus_tag="PL000_13300"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HNC89925.1"
                     /translation="MKTFGCQSMYAPIRRMVLKHPREAFISQANLEAQWEKLVYFGCP
                     DFENSLAEYERFTGILEHFDFEIHYAPMDASTSLDSIYTHDPLVISNRGAILCSMGKE
                     ARKPEADAAEKYLHELGVPILGRIHGSGKLEGGDVLWVDERTLAVGQGFRTNAEGLRQ
                     LRELLSDAVDEIYPVQLPYWTGPQDCLHLLSFISIVDKDLAVVHSRLMPVPFREWLLE
                     RGFRFVEVPNEEYDNLGCNVLAVGPRQCVMLSGNPITQKRLEEAGAQVWTFDGKDISF
                     KGTGGPTCLTRPILRS"
     gene            complement(7327..7725)
                     /locus_tag="PL000_13305"
     CDS             complement(7327..7725)
                     /locus_tag="PL000_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC89926.1"
                     /translation="MEIKITTMKGRVPVQVMHVHGELNSATYEAFQSKANELIKSGAQ
                     YILLDLTNSPFISSAGLRTIHSIYNQLRAAKPDKDDEEIRKGISAGTYKSPYIKVLNL
                     SSDAAKAFNLGGFDMYIESHTDLLKAVASF"
     gene            complement(7830..8852)
                     /locus_tag="PL000_13310"
     CDS             complement(7830..8852)
                     /locus_tag="PL000_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89927.1"
                     /translation="MQLDAIIEVAIGLVFVWLVISITTMEAQNRLGVWMNGRAKHLEK
                     SIHSMLKDKTLVDRFYDHPLIVELMLKDDDGNLKLDKKGKPRRPDYIPTQVFATAVFE
                     VLMNAGKGEKDIAAETLSIPDMTANFKTLMAKNEVLPYVFPNMEKSMETLQRNTKEFD
                     DKLAEYRRNIEGWFDNVMVQASGWYKIDSQWKAFWIGLALAVFFNVDTIFIAQKLWQE
                     PTARAVIVAQAQTEAQGESSIDSISLDNVKGLVFPVGWTTTPLAEGTSCGLVSFMDNR
                     VVISSEGNCLAVTSLPAFNNGWGLMVKIFGYLLTAFAAAQGAPFWFDILRKLVGFKQQ
                     TSSTSK"
     gene            complement(8931..9821)
                     /locus_tag="PL000_13315"
     CDS             complement(8931..9821)
                     /locus_tag="PL000_13315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC89928.1"
                     /translation="MQNKILPYLALAVGISALSLSAMFVRWAEAPGAVTGFYRLLFST
                     IFLSPLFIRQQKNLEPIEKKYLLFPLLGGLFTAFDFGFWNSSLKFTTAANATLLGNTS
                     PLWVALFAFFVLHEKLRRSFWVGLVIALTGAVLVMGSDYLKHPTLSLGDLMASTAAVF
                     YASYQLVTQRGRKHIDPLRYTWLVGVSAVIGMFIMNLVLGNQLTDYPLKSWLIFLATA
                     VVSQMIGYLAITYALGHLPAYVVSPTMVGQPVLTAVLAIPLLGEVPSTIQWVGGAIAL
                     VGIYLVNQSHLQAQEELPNA"
     gene            complement(9839..10582)
                     /locus_tag="PL000_13320"
     CDS             complement(9839..10582)
                     /locus_tag="PL000_13320"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC89929.1"
                     /translation="MKASSVHHILFDLGGTLMHSRGDWTPILERADQALADSLRQHEI
                     ELDTRVFRARLHEYYDQRDQDFQETTYHFVLRELLKDLGYVDVAEPVLRSALDALYSV
                     TQSNWMLEDDSLDTIQALRSNQYQLGIFSNAGDDQDVQELIEDFGIRSYFDFVLTSAA
                     CFYRKPHPRAFEIALAQWNISPEEAVMVGDSLEADIQGAKNLNMKSIWITRRAQFKAD
                     EMQRFKPDFSVRSLKELLPTLERISITRA"
     gene            complement(10621..12672)
                     /locus_tag="PL000_13325"
     CDS             complement(10621..12672)
                     /locus_tag="PL000_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89930.1"
                     /translation="MKVFQFLFRVGFSLSIAFALTFSSAIIFFDYSGYETALDRAALV
                     SFPTLAIGFLLFQAFPTLWQWIRQRRPAVLFLLGGLAGIGAAAVVLPFAVSSVYYLGT
                     LAFALALFALMLPAAPMVERMRNRLWGYSFGFLLSLLFAYGAVGFLAGALHSIFNVLV
                     FTVILVGAGSVAGNYLFIRAADSFRGGFLRQPLNLLLALALPAFLAAVIAVGMQFPPM
                     LLRQHLMVPREWFGLFLSAALMTGVWGLGLLEQFETRGFHNRLEKTRLFEFIKPNLPG
                     VYAGGMFFLINLVIARILNHPALSVNTVLFESDAGPWLFILGSPTGDALNRSVHPLAL
                     TTIRYLVRVFAIFMGDAWQLAPILVAAMLSGLCVFMAWLFVKRAVGKSSYAFIFAVML
                     GSTAAHLFFGSLTDTYIFGVTSLIFFWLLIQAQENRFAVLVPAGVLVFGVTVTNIAQA
                     MIGLFCSKFGFRRLIRYGIMVLAVSIVLTAFTSMVYPRWQTFFFVPADLAFEGNFVKP
                     VYESPTERLLERFKLVGRSMVLYGVTAPSPIEVIAEKDPRPTIDLKTFDAPNNAYASY
                     KGFANIPLALWLALLAGAFIFFMKGLRSSAHLSLMLGLLGSLAFNFILHMNYGTELFL
                     YALYWTYLLVFFLALAYAGLAGRPWFEAILTLFILALMANNLGFIFAVLRGLAPFFAS
                     V"
     gene            12858..14831
                     /locus_tag="PL000_13330"
     CDS             12858..14831
                     /locus_tag="PL000_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89931.1"
                     /translation="MSGLIGQSLGRYQIVELIGEGGMATVYKARDARLDRDVAIKFIR
                     PEAVPPDQFEIILKRFEREAKSLAGLSHPNIVGVIDYGEHDGLPYLVMVYLPGGTLKE
                     RLGAPMYWRDAVRFIIPVARALEYTHEHSIINRDVKPSNILLTEKGQPMLTDFGLVKI
                     FEGKNATHLTASGAGLGTPDYMAPEQWIGEATAASDQYSLGVVLYEMITGHRPYISDT
                     PAGILLKQINDPLPFPKTYIPDLPQSVESVLMKVLARKPTDRYADMHAFANELQNLLD
                     GREVTATGIKTKQLREQMTGKIEKPKLEQKPAEEEKAPEKTHLQADKKTASPADPQPA
                     SPKKKISSPWLAVLGGFVLLGLCGACWLLNARFNFFGGTTNPTATQSVTLATDVPTEE
                     VVEEILPTETSQPASEPTEAPATEISLPAEITDGKNISMRLVPAGEFIMGNDDGNTEE
                     SPASKVQVAAFYMDKYEVTNDMYAECVAAGVCKLPEKPGSATRSSYYNNPSFANYPVL
                     YVNWTMAKTYCEWRDARLPTEAEWEKAARGMDDQRAYPWGNDFNCNFANHSGCVGDTA
                     PVNQYNSGQSPYGIYGLSGNVWEWTSSLFAFYPYSATDGREDPTATGFRSVRGGSWNI
                     FGGSGGNIRIDTRYKLDPVYFGAYVGFRCALTK"
     gene            15118..>16627
                     /locus_tag="PL000_13335"
     CDS             15118..>16627
                     /locus_tag="PL000_13335"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC89932.1"
                     /translation="MPLLIGQTFGRYQILELLGEGGMATVYKAYDTHLEREVAIKVIR
                     RDAFPPDDMNMLLKRFEREAKSLGRLSHPNIVGVIDYGEHDGSPYLVMVYLPGGTLRE
                     RLGKPIAWRAAVELILPIAHALEYVHDRNIINRDVKPSNILMTEKGQPMLTDFGLVKL
                     FGEQEKESTHLTSSGTGLGTPDYMAPEQWTGEAIPQSDLYSLGVVLYEMITGRKPFTA
                     DTPAGVLLKQATEALPLPRAYIPDLPQEVESVLLKVLAKDPEDRYPNMHTFIGELQNL
                     LAGREVSATSIKADQLRKKMTGRTEATTFDPATGRTQKAFPTLFAVLGGLGALMVIAV
                     IGVLIYAGNGGTALPFLAAPTQTSQPTAIPSATNTAIPPTETVEVTAAPTETLVPTET
                     AIPSEIRDERNVPMIYIPAGEFTMGSNDSGDVASKPEHKVELGAFYIDKFEVTNERYD
                     ACRYADECVAPQQLGTATRNNYYTNAVFKDYPVIYVDWKWAKAYCEWRGARLP"
     assembly_gap    16628..16727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16728..17177
                     /locus_tag="PL000_13340"
     CDS             <16728..17177
                     /locus_tag="PL000_13340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC89933.1"
                     /translation="VDWKWAKAYCEWRGARLPTEAEWEKAARGTDGRLYPWGNEKPDC
                     NFANITGCVGDTTPVDQHELGKSVYGVYGMAGNVMEWTSSLFDYYPYDAADGRENPDS
                     TKERIVRGGAWNLFGGNNGNVRVDTRVKLPPEYYGAYVGIRCAKSAQ"
     gene            complement(17240..>17878)
                     /locus_tag="PL000_13345"
     CDS             complement(17240..>17878)
                     /locus_tag="PL000_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC89934.1"
                     /translation="SVGCGWSSINADIRIVNAQTKELCAKDEVGEIWVSSTSVAQGYW
                     KRPEETEKTFQAYIASSNEGPFMRTGDLGFIYNDQIHITGRIKDMVIIQGRNFYPQDI
                     ELTMEKCHDALRPSFGAAFAVEANQVDHLVVVQEVKREYRKSNDFEGIANAIRMAVAK
                     NHGLRVQAVVLIMPSTIQKTTSGKIQRNATRESFLKGNLEVLYEWRAPGIFS"
     assembly_gap    17879..17978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17979..18574)
                     /locus_tag="PL000_13350"
     CDS             complement(<17979..18574)
                     /locus_tag="PL000_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC89935.1"
                     /translation="MIQNPEEAVSLVEVVRFYADRIGNELAYMYLVDGEDQEAPLTFS
                     ELDRQARAFAATLQQHTKPGDRALLLYPSGLEFIVAFFGCLYAGVVAVPTIVPHLKRA
                     TPRFRAMLSDADAVVACTQMELFDKLKQLFDEYPEFHDLKWVVNEKLEAGVESQWQEV
                     ENKADDLAFFQYTSGSTSTPKGVMITHFNLIETVRDLQR"
     gene            complement(18571..18822)
                     /locus_tag="PL000_13355"
     CDS             complement(18571..18822)
                     /locus_tag="PL000_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC89936.1"
                     /translation="MEEIEIKEKLKKFICTEIMNDADYPLKFDEPLITSGLIDSFSLA
                     QVGVFVEIEFQIYIPDPELTVDNMNTIDAMAARIKEKMG"
     gene            complement(18824..20278)
                     /locus_tag="PL000_13360"
     CDS             complement(18824..20278)
                     /locus_tag="PL000_13360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC89937.1"
                     /translation="MTDLFSNLSITSNGFFFFVLAALGVYYLVPKKAQNHVLLIASYL
                     FCFTWDWTFAAILLASSIVNYWVALKLDKAEVTAKTRWVWIGVAANLGVLLFYKYFDQ
                     TSVYFFKALLRIGLTNELLSLDLLQPIGISFYTLQAISYLVDVYRKQIPANHSFVEVS
                     LYLGYFPKLLAGPIERAQNFFKQLQADVEIGNDQLVESFIKIFVGLVRKLVISETIVR
                     MIPLGVFAAPQKYSMPDLLFWWLTFAFVIYNDFAGYTSIARGVSGLFGIQLSPNFEQP
                     FFSTSYIDFWNRWHISLSNWLRDYIYLPVSRAMLRRNPSGRYWPNLVVPPLVTMIVSG
                     IWHGAAPHFILWGALNGLLQGYERVQKTFVKKTTVQPPRWRRAVSISVTLLVLILINA
                     PFKMDFSQTLAFWSGLFRWGIIQTLSVHQIIKPILAIGLSFFIDVMQLPAKDELGLLR
                     LSKPVKVFLLTVGLLLLFLSTRQAAVAPFIYQEF"
     gene            complement(20346..>21188)
                     /locus_tag="PL000_13365"
     CDS             complement(20346..>21188)
                     /locus_tag="PL000_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89938.1"
                     /translation="REVQDGQIDCIILGDSQVLYGIDPTIVEQAYFDSTGNTIHCQNF
                     GLGGLKPITAYPLAKLLIKNFHPAIIVFGTDLFDYTAVQAGSDSSIMSSPWVKYQLDD
                     FSADGWLIENSNSYRFYLGADRYLLNGEENADHIGLNGHSLKFSEKTNMSQQEQVDYF
                     DSLLDRPEITGEQLDGLHELLALNSAETKIVLFETPVDPIFFTLKEFSYNRKVYPDFR
                     NMLENEAAYVHTDLWLTQETVQIPTDGWYDIVHFNKVGSEYFSRLLGEHLSSIQIPIS
                     GQIP"
BASE COUNT         5490 a         5569 c         5228 g         4701 t
ORIGIN      
        1 gtgtgacgcc cgaaatgctg ccgcgtgcca tgcgttggat gttccccatt ttcaagttgc
       61 tggtgcgcga cgatggcggc aagtctgcgg cgaaggcggc gcgttcgtct gtgtatcttg
      121 cgtcttcgcc agaggttgaa ggcgttacgg gaaaatactt cgatacgaat tgtaaagagg
      181 cagcatggtc gccagcggtg atggatgatg atatgcgaaa aacgctttgg gagcaggtgg
      241 aacaaataac aaaacggcag tcgggatgac tgccgttttg atttggggtt gaagttttat
      301 ttttttctct ttgcgccaga gcgtttcttc agatcgtcaa cggttaacat ggagatgctt
      361 tcctttgcct tttgatcgcg caactgatca tgtttctcga tggcgcggtc gataatggcg
      421 gccttcaagt tattcaaaag ttccttcctg gttttataac caaaggtcgg caaaacccaa
      481 tggaagctct tttgcagatc gatcatcatg gcaaagggac gatcatcagc gctggtatca
      541 ataatgggca aatacggaat cttgaattgc ttgaccgtag cttccatttc caaaggcgtg
      601 gatttgggat tggtcacatc tacaatgaca aacatgctca tgcccgccaa agtctgaacg
      661 gtttcggtgt aatctttatc ctgaggcgaa tcgaaatcga acacaatggg gacgaaatcg
      721 aaagtgcgca gtttttcgcg caatccatcc agcacggctt tgcgctcggg gagtgagaac
      781 ctgcccaaaa tcagcacatt tttatctgtg aaggcattca tgaagttgcg aattttctta
      841 ttgttgtaaa caaggtacac aaactgggca acttccagat catccacggt aatggcgggt
      901 tcggatggcg gtgtgatgat caaatcctgc tgcgattttg gcgttccgat caaatcccag
      961 gcagaggttc catacacgta acagttcttg aaattggctt tctccaccac agcgcgaagc
     1021 agggatgtat tggcaaggta cgagccatgc agatcggcac ttgtaagatc agcatcgcga
     1081 aactcagcag tatccagatt ggaaaaactc aagtttgcgc caaccaattt tgcctcggcg
     1141 aactcaacca tctggcaatc ggtttctttg atatttgccc ttgtaaggtt tgcacgccag
     1201 aaagatgcgc ccgccagcgt ccgcccggaa aaatccacac cggtcaggtt tgagttgacc
     1261 atcttcacat tgaccaatga agccttctta aaagtacaag aagagagatc gcactgggtg
     1321 agataagtcc catttaagtt tgtttcgttg aagttcgcgt ttttgagaat cgcctgatca
     1381 aatttagcgt cagaaagatc cgaaagaaaa agagaggcgt tggtcaaatc tgctttttca
     1441 aagttggctt cctgcgagag ggtaaattca atttttgcat tccgcaaact tgccccttga
     1501 aacaaagccc gttgcaaagt ggcgccactt aagttggctt tgtcaaaatt cgctttgcgc
     1561 gcatcgacgt tatacaaata agcaccgcgg agatccgttc ccacgcattg ggcatttctg
     1621 agatcagagt tttcaagaaa caccgcccgc aggtcaaagc cgcccagatc agcgttacga
     1681 aatgagaggg tgctcaaatc tggttttatt tttggattct ttttccgcca ggcattccaa
     1741 actttgcgtc cctgcgaaaa tatttcacga tgcttctggt ctgccataca aactcctttt
     1801 aacaacgcta aatttcaagc aaataaaatg ccatggcaca catttccttc actcgatcaa
     1861 agctcacagc tactttttat tttctttcgg tagaaaatcg aacggtggac gctgattcaa
     1921 ttttacaaga tgcgcacccg cttcacccac atgcaaaacg gtcattgaac ctgtatcaca
     1981 ggccagccgc tgaaaatggt caagcggcat tccgatgaaa tgagaaacgg ccagtttgat
     2041 cgggtcggca tggaacacaa ccgcaacaat atcctgcggc tttttatgag tttgcaccac
     2101 cctctccaac acatttgcaa ttcgcagttg tgcctcagga aacgactcgc cttcgggaaa
     2161 acggaatcgc gacggcgcat tctgcacgat tttccatgcc tttgtcaggc caagcacgcg
     2221 ccatgatttt ccctgccatt ttccgatatc ggtatccatg agatcaggtt cctgaatgat
     2281 cttcaaattt cttgcggatg cgattggcgc agcagtttcc atcgcccttt ccagcggact
     2341 tgaataaatg gccttgatcg gcacatcctt cagcgcatca cccaacgcct gagcctgctt
     2401 ctgcccacgt tcattgagat gtacccctgg cagtcggcct gccattttcc ctgttttgac
     2461 atattcgttt tcgccgtggc ggatgagaag taaaagaggc attgtttatt ttccaatcaa
     2521 aagttttttg ccgccagcct catcacatca agataaggga cggattcatg ataataatcg
     2581 caaaacgcct cccacacctg ctcattggga agcgcgccag ggtgacgttg agatacttct
     2641 ttgaacgcat caaaaaggct gaggccttct gcctctttca acgcagcaag gcaaaacatt
     2701 gttgaccgat ttactcctgc cccgcaagaa accaatatgt tatgtccctt ttccttttcg
     2761 gccagaacga aatccacacc ttgtttaatg agatgatgcg gcgtacgggc aagatcatcc
     2821 acctgtagaa acagggaatt gattccctcg tgttgaatcg gttcggcaat ttgcagcatg
     2881 gaccgaatca acttgcgatc aagatacccc ttgtcgaggg tatctctgaa cccgccaata
     2941 aaaagccagg ggcgaatttc atccatgcta tttgatcttc cgcttcattt caacgatctg
     3001 ttcttcgctt aagctgggga catgttcggt gcgttcgatg ttcagggctt tgcgctgggc
     3061 ttcgcgccac atctccaacc gtgcagtgac ctgtgactcg tatccctcct gtgaaggcga
     3121 atagaaatac gtgccgagca atcgcctggg tagatattgc tggggagtga aatgcccctc
     3181 ctgatcgtgc ggatacacat aatccttgcc atgacccatt gccgcggcat cgcgatttcc
     3241 gtccatgagg tggcgcggaa cggagacctg cccctcttct tcaatttttt tcatcgcgct
     3301 gaaataagcg cccgcgctgt tggatttggg cgcggtggca agatagaggg ttgcctccac
     3361 aatcgggtaa atcccctcgg gcagaccgat atagtcaaat gcttgtgcgg cagaagaagc
     3421 caccaccaac cccatggggt cggcaagccc aatatcttca ccagcaagaa taatgagacg
     3481 acggatgatg aacttgggat cttcacccgc atagatcatc tttgccagcc agtagagggc
     3541 agcatcgggg tcacttccgc gcaccgattt gataaacgcg gagatggtgt cgtagtgcgc
     3601 gtcaccatct ttatcgtaga ggactgcccg ccgttggatc gactcttgcg ccacatccag
     3661 cgtgatatgg atgatgccat ccctgtctgg cgtggtggat tccactgcca actcaagtgc
     3721 gttgagcgcg ttgcgcgcgt cgcctgaggc aacttcaatt aagtgggacc gtgcatcgag
     3781 gtccagtgtg atgcgcttgt ttccgtagcc gcgttccttg tcggagaggg ctcggtcaag
     3841 cagaacaccc gtctcggtct ggttcaaatt ccgcaattga aagacacgcg aacgagagat
     3901 caaagctttg atgacttcaa agtatggatt ctctgttgtc gcgccgatca acgtgaaagt
     3961 tccattttcg acatgcggca ggagtgcatc ttgctgagct ttgttccaac ggtggacttc
     4021 atccacaaaa aggatggtac gctcgttatg caggcggcgg cgttcgagag cctcatcaat
     4081 gacaacgcga agatccgcct tgcctgcaag gatggcagag atggtaacaa aatgactctt
     4141 cgtgctgttc gctatgactt gtgcgagtgt ggttttgccc gtgcctggcg gcccccataa
     4201 aatgatggac gaaaaaagtt tatcggcttt gatggcgcgc tgtaaaagtt ttccctcgcc
     4261 gacaatatgc tcctgcccga tgtattcatc cagcgtacgc ggacgcatcc gcgcggcaag
     4321 cggtgcttcg gttttcatgc gttcctgcat ggcgtgatca aaaaggtcgc tcataaacta
     4381 gtttccgttc ctttgcagcc aatctaaaaa ttcccttcct gaaaggattg gaacggagcg
     4441 aaactccttc aagtcaagca aatgtttatc tccgcttaca ataaaatcca cattacccgc
     4501 aatggccgcc tcaagaaatt tatcatcttt tggatcatct tcaataaacc gaattttttc
     4561 ttcaggtatc acaaactctg aaaactgata aatgtaggtt gtgattttat ctatcgtttc
     4621 ctgtttcaac ccaaatttag gtcggttgag aacctcaaaa tactcactca aaatatccgt
     4681 ggtaacaaca acagaaaata cgccagcgtc ccatttctct aaaaccaaaa ccagcgcacc
     4741 accaagcgcg ctggaaatca aaatatttgt gtcgagaact gcgcgtttca ctgctttcca
     4801 tcccgcacag cacggatttc agcttcgata tcttcctggg tgatatttaa ttttttcgca
     4861 gttgctcttg cgtcctccaa tgcctccttg aaatcagaac gggctttttc actttctggc
     4921 aggctgattt tcagaaaccg aatgaacgcc agtacatccg cctgacgaga ttgagggagt
     4981 gtagcaactt cacgcaaaag agtttgttca aaggtagtca tgattcacct taagttcaat
     5041 tatacaactt attaaaaggg gtacttgcaa ggcattagat atcgtttgat tataatggaa
     5101 gtctctacag aggaaacccc atgaaaaaat cccccactga aatttctgaa gccgttgaag
     5161 cggcccgcga agaattgatc gcttttctgc aaaagctggt gcaaaccgcc agcctgccaa
     5221 atcacgagca cgatgtccaa aaccttgtgg cgcaaaaatt acgttcgatg ggcatggaag
     5281 tggagatcat cccttcgaat ttcgacaatc tgaaaggcca ccccgctttt ggggatgatg
     5341 gcttctcgcc aacagatcgt gtcaacgtga tcggacgctg gcccgggact cgcactggcg
     5401 aaggaaaatc gatcatcctt aacggacacg tggatgtggt tccagtgggc gatctctccc
     5461 tttgggtgga ttcgccctgg agcggtgtta tccgcgacgg gaaaatgtat gggcgcggct
     5521 cctgtgacat gaaatcgggt ctgtgcgcgg gtatctttgc agtggacgtg ttgcaaaaac
     5581 tcggttacca gcctgccaac gatattctga tcgaaagtgt cattggcgaa gaatcgggcg
     5641 gcattggcac attgaccacc attgtcaaag gttacacggc tgacgctgtg atcctgcttg
     5701 agcccacgcg gctggagctt tctccagtgc aatcgggcgc actgacattc cgcctgaccg
     5761 tgcctgggcg ttcgatccat gcggcgatga aggcttacgg tgtgagcgcc atcgaaaaat
     5821 ttgccttgct gttgaatgtc atcaacaaac tcgatgcaga acgccatgcc agcttcaagc
     5881 atccattgtt tgaagatccg cacaacattg cgccgatcaa cattggcaca gtacgcggcg
     5941 gcgagtggca ttccactgtc cctgaagaag tggtggcaga gggacgtatg ggggtgttcc
     6001 ctggggaatc gaatgaagcg gcgcggaaaa tgttcgaagc ggcgattcag accgcagcgg
     6061 agtctgaccc gtggttgagt caaaatccgc ccagggtgga atggttcgag ggtcaattcg
     6121 aatcgggtga aactccgctt gaacatccgt tgattcaaaa tctcgctgcc atgcatgaac
     6181 aggtcacggg gtcggcggca actctgcgtg gtgtaaccta cggctcagat atgcgcctgt
     6241 tcaccaacca tgcgaaaatt gcagccacac attatgggcc tggtgacgta ggcatggcac
     6301 atgcggtcaa tgagtttgtg ccgctcgatg aggtggtgac tgcggcgaag gtcgtggcaa
     6361 atacagtcgc acagtggtgc ggaggagaat tcaaatgaaa acatttggtt gtcagtccat
     6421 gtatgcgcca attcgacgga tggtattgaa acatccgcgt gaggctttta tttcgcaggc
     6481 aaatcttgaa gcgcagtggg aaaagctggt gtattttggc tgccccgatt ttgaaaattc
     6541 gctggcagaa tacgaacgat ttacaggcat actggagcat ttcgattttg agatccacta
     6601 cgcgccgatg gatgcaagca ccagcctcga ttcaatttac acccacgacc cgctggtaat
     6661 ttcgaatcgc ggcgcgatcc tgtgcagcat gggcaaggag gcacgcaaac cagaagccga
     6721 tgccgcagaa aaatatttac atgaattggg cgtgcccatc cttgggcgga ttcacggaag
     6781 tggaaaattg gagggcggcg atgtgctctg ggtggacgaa cgcacactgg cagtgggaca
     6841 gggctttcgc accaatgcag agggcttacg tcaattgcgt gaactgctca gcgatgccgt
     6901 ggatgaaatt taccccgtac aactccccta ctggacgggg ccgcaagatt gtttgcatct
     6961 gctgtctttc atcagcatcg tggataaaga tctggctgtc gtacattcgc gcctgatgcc
     7021 cgtgcccttc cgcgaatggc tgctggaacg cggctttcga tttgtggaag tacccaatga
     7081 agaatacgac aaccttggct gcaatgtgct ggcagtgggg ccgcgtcagt gtgtgatgtt
     7141 gagcggcaat ccgatcacac aaaaacggct tgaagaagct ggggcgcaag tatggacctt
     7201 cgatggaaaa gatatttcct tcaaaggcac aggcggtccc acctgcctga cgcgcccgat
     7261 attgagaagt taaaaaacaa cggcggcctc taggccgccg ttgttttttt gtcttttgca
     7321 actcacctag aaagatgcaa ccgccttcaa caagtctgtg tgggattcga tgtacatatc
     7381 gaacccgccc aaattgaaag ccttggcggc atcggaggaa agattcaaca ccttgatgta
     7441 gggtgatttg tatgttcccg cactgatgcc tttacggatc tcttcatcat ctttatcggg
     7501 tttggcggcg cgcaactggt tgtaaatcga atgaatggtg cgcaggcccg cactgctgat
     7561 aaagggggaa tttgtcaggt caagcaaaat atactgggca ccacttttga tcaattcatt
     7621 agccttgctt tgaaacgcct cataagtagc tgagtttagt tcgccatgca catgcataac
     7681 ctgaaccggg acgcgtccct tcattgttgt gatcttgatt tccatgcaat cctccgatgc
     7741 aaaatttggc cgatttcttt gtgtagttta acgggtttcg gcctgtctcc cgaaatcgaa
     7801 acgaatttgt gatttactga attcaacaac tattttgaag tggaagaggt ctgctgcttg
     7861 aaaccaacca atttgcggag gatatcaaac cagaacggcg caccctgcgc cgccgcaaat
     7921 gcggtgagga ggtagccaaa aatcttcacc atgagacccc agccattatt gaaagcaggc
     7981 aggcttgtaa cagcaagaca attcccttca gagctgatca cgacacgatt gtccatgaag
     8041 cttaccaaac cacaggaagt cccttctgcc agcggggtcg ttgtccaccc aactggaaag
     8101 accaaccctt tgacattatc cagtgaaatg gaatcaatgg acgattctcc ttgcgcctcg
     8161 gtctgagcct gcgcaacgat cacggcccgc gccgtgggtt cctgccataa tttttgagca
     8221 ataaaaatgg tgtcaacatt gaaaaataca gccagggcaa ggccgatcca aaaggctttc
     8281 cattgggaat cgatcttgta ccaaccgctc gcctgcacca tgacattatc aaaccagcct
     8341 tcgatatttc ggcggtattc ggcaagcttg tcgtcaaatt ccttcgtgtt gcgttgaagc
     8401 gtttccatac ttttttccat atttggaaaa acatagggaa gcacctcgtt ctttgccatc
     8461 agtgtcttga aattggcggt catatcagga atcgacaggg tctcggcagc gatatctttt
     8521 tcacctttgc ccgcgttcat caaaacctca aacaccgccg tggcaaacac ctgcgttgga
     8581 atgtaatccg ggcggcgtgg tttgcccttt ttatccagtt tcaggttccc atcatcgtcc
     8641 ttcaacatca attcaacgat cagtggatgg tcatagaaac gatccaccag ggttttatct
     8701 ttcaacatgc tatgaatgga tttttcgaga tgtttggcac gtccattcat ccaaacacca
     8761 agcctgttct gggcctccat ggtggtaatg ctaatgacca gccacacgaa cacaagcccg
     8821 atggcaactt cgataatggc gtctaattgc atgatggtct ccttgggtct gatggttaca
     8881 cgccttacag caccagtata taagcggaaa ggcttctttt caaggtcagg tcaagcgttg
     8941 ggaagttctt cctgagcttg caaatgtgat tgattgacaa gataaattcc aaccagtgca
     9001 atcgccccgc ccacccactg aatcgtgctc ggaacttctc ccaaaagcgg aatcgccagc
     9061 acagccgtta aaacgggctg tcccaccatt gtaggcgaaa ccacataggc tggcaaatgg
     9121 ccgagtgcgt acgtgatcgc cagatacccg atcatctgag aaacgacagc cgtggcaaga
     9181 aagatgagcc atgatttcaa tgggtaatcg gtgagctggt ttcccaggac aaggttcatg
     9241 atgaacatgc cgatcacagc gctcaccccc accagccagg tatagcgcag cggatcaatg
     9301 tgctttcgtc cgcgctgagt gaccagctga tacgaagcat aaaaaacggc ggcggtgctc
     9361 gccatcagat cgccgaggct gagggtgggg tgcttcaaat agtcactgcc catcacaagc
     9421 acggcacccg tcaatgcaat gacaagtccc acccaaaatg agcggcgtaa tttttcgtga
     9481 agcacgaaaa atgcgaacaa ggccacccac aacggcgaag tattaccaag caaggttgca
     9541 ttggcggcag tggtgaattt caaagatgaa ttccagaatc caaaatcgaa agcggtgaaa
     9601 agcccgccca aaagcggaaa gagcagatat tttttctcaa tgggttccag attcttttgc
     9661 tgacgaatga aaagcggcga aagaaagatc gtggaaaaca acagacggta aaaaccagtc
     9721 accgcgcctg gggcttcggc ccaacgtacg aacatggcag aaagactgag cgcgctgatc
     9781 cccaccgcca gcgcgagata tggaaggatt ttattttgca ttaaagaatt gtactctgtc
     9841 aggcacgggt gatggagatt ctctcaaggg tcggcaaaag ctccttgagg ctgcgcacgc
     9901 taaagtcagg tttgaagcgc tgcatttcat ccgctttaaa ttgggcgcgg cgcgtgatcc
     9961 agatcgattt catgttcaaa ttctttgcac cctgaatatc ggcctccaga ctatcgccca
    10021 ccatcaccgc ctcttcggga gaaatattcc attgtgccag tgcgatctca aacgcacgcg
    10081 gatgcggctt gcgataaaaa caggcagcgg aggtcaacac aaaatcaaaa tatgaacgga
    10141 tgccaaaatc ttcgatcaat tcctgcacat cctgatcgtc gcccgcgttg gaaaaaatgc
    10201 caagctgata ttgattggaa cgaagcgcct ggatcgtatc cagcgaatca tcttcaagca
    10261 tccagttgga ttgtgtgacc gaatacaacg catccaacgc ggacctgagg actggttcgg
    10321 cgacatcgac atatcccaag tccttgagca attcccgcaa aacgaagtga tacgtggtct
    10381 cttgaaaatc ctgatcgcgc tggtcgtagt attcatgcag gcgcgcccga aagacacggg
    10441 tatcgagttc gatctcatgc tggcgcaggg aatcggccag tgcctgatcg gcgcgttcta
    10501 aaatgggagt ccaatcgccg cgtgaatgca tgagagtccc gcccaggtca aagagaatat
    10561 ggtgaacaga agatgctttc attgtatttg ccctactcaa aaaaacagtg aactaccttt
    10621 ctacactgaa gcaaaaaacg gagccaatcc gcgcagaacg gcaaaaatga atccgaggtt
    10681 gtttgccatc agcgccagta taaagagggt caggatcgct tcaaaccatg gcctgcccgc
    10741 cagccctgca tatgccagcg caagaaagaa caccagcaga tacgtccagt acagggcgta
    10801 caggaacagt tccgtgccgt aattcatgtg caggatgaaa ttgaaggcca aactgcccag
    10861 caagccaagc ataagcgaaa gatgcgccga cgaacgtaac cctttcataa aaaatatgaa
    10921 agcccctgcc agcagcgcca gccacaaggc cagcgggata ttggcaaacc ctttgtagga
    10981 ggcgtaggca ttgttgggcg catcgaaggt tttcaggtca atggtggggc gcggatcttt
    11041 ttccgcgatg acttcgatgg ggctgggcgc ggtcacgccg tacaaaacca ttgaacggcc
    11101 caccagtttg aagcgctcaa gcagccgctc ggtcggcgat tcgtacacgg gtttgacaaa
    11161 atttccctcg aaagccagat ccgcggggac gaagaaaaag gtctgccagc gcgggtaaac
    11221 catgctggtg aaggcggtca acacaatgct cacagccagc accatgatgc cgtagcggat
    11281 caaacgccga aacccaaact tgctgcaaaa taagccgatc attgcctgcg caatgttggt
    11341 gaccgtaacc ccaaaaacca gcaccccggc agggacaagg acggcaaaac ggttttcctg
    11401 tgcctgaatc aaaagccaga aaaagatcaa cgatgtgaca ccaaagatat aggtatcggt
    11461 cagagacccg aagaacaaat gcgcggcggt cgaacccagc atcaccgcaa aaataaaggc
    11521 ataactgctt ttgccaacgg cgcgtttcac gaacaaccag gccatgaaca cacacaaccc
    11581 actcaacatg gccgccacaa ggatcggcgc aagctgccat gcatcgccca tgaaaatggc
    11641 aaagacacgc accagatagc ggatggtcgt caatgccagc ggatgcacgg aacggttcaa
    11701 tgcatcgcct gtggggctgc cgaggatgaa cagccacggc cccgcatcag actcaaaaag
    11761 gacggtattc acactcaacg cggggtgatt caagatccgc gcgatgacaa ggttgatgag
    11821 gaaaaacatg ccgcctgcat acaccccagg cagattcggc ttgatgaatt caaacagcct
    11881 ggttttttcc aatcggttgt gaaatccacg agtctcgaac tgctccaaca gacccaaccc
    11941 ccacacaccc gtcatcaaag ccgctgaaag gaataaaccg aaccactccc gcggaaccat
    12001 gagatgttgc cgcaaaagca tgggcggaaa ttgcatcccc accgcgatga ccgccgccaa
    12061 aaaggcaggc aatgccaatg ccagcagcaa attcaaaggc tggcgtaaaa aaccaccgcg
    12121 gaacgaatcc gctgcacgga tgaagagata attccctgcc acactgccgg cacccaccaa
    12181 aatgaccgtg aataccaaca cgttgaagat cgaatggagc gcacccgcca aaaagccgac
    12241 cgcgccatac gcaaaaagca gggaaagcaa aaagccgaag gaatatcccc aaaggcggtt
    12301 acgcatccgc tccaccatgg gagccgcggg caacatcaaa gcaaagaggg ctagcgcgaa
    12361 ggccaatgtc ccaaggtaat aaacgcttga aactgcgaac ggcaaaacca ctgctgccgc
    12421 gccgatgcct gccagcccgc ccagtaaaaa caggacggcg ggccttcgtt gtcgaatcca
    12481 ctgccacagg gttggaaatg cctggaatag caaaaaacca attgccagtg tgggaaaaga
    12541 aacgagcgca gcgcggtcca gagccgtttc gtagcctgag taatcaaaaa aaatgatcgc
    12601 cgaagagaag gtcagggcaa aagcgatgga caggctaaat cccacacgga acagaaactg
    12661 aaagactttc actcggcact tctcccaaac gaaaccgcag atacaaccat aagatgaccg
    12721 tattttaccc gatgaccaca ccccctaatt tggcatggat tcataatggg caaaaattga
    12781 cttgaccgtt aaattagaca tataatgaat cacatcggca cacttcaaac gctcggaaga
    12841 gaaaaaggga aaaacctatg tctgggttga tcggtcaaag tttaggacgt tatcaaattg
    12901 tcgaactcat cggcgaaggc ggcatggcca ccgtgtacaa ggcgcgtgat gcccgcctcg
    12961 accgagatgt cgcgatcaaa ttcattcgac ctgaagccgt gcctcccgat caattcgaga
    13021 tcattctcaa acgtttcgag cgcgaagcca agtcacttgc gggactttcg caccccaaca
    13081 tcgttggcgt gatcgattac ggcgagcatg atggcctgcc gtatctggtc atggtatatc
    13141 tgccaggggg cacactcaag gaacggcttg gcgcacccat gtactggcgc gatgccgtcc
    13201 gcttcatcat ccctgtggcc cgcgccctcg aatacaccca cgaacacagc atcatcaacc
    13261 gtgatgtcaa accatccaac atcctgctca ccgaaaaagg tcagcccatg ctcaccgatt
    13321 ttggcctggt gaagatcttt gaaggcaaaa acgccacaca cctcaccgcc tccggagcag
    13381 ggttgggcac gccagattac atggcccccg aacaatggat cggcgaagcc accgccgcat
    13441 ccgaccagta ttcgctcggt gtggtgctct acgaaatgat cacaggccac aggccctata
    13501 tttcagacac ccctgctggc attctgctca aacagatcaa cgacccgctg cccttcccca
    13561 aaacatacat ccccgatctg ccgcaaagcg tggaatcggt gctgatgaag gtgctcgccc
    13621 gaaaacccac cgaccgctac gccgacatgc acgccttcgc aaacgaactg caaaacctgc
    13681 tcgacgggcg cgaagtcact gcgactggga tcaaaaccaa acaactccgc gagcaaatga
    13741 caggcaagat cgaaaagcca aagcttgagc aaaagccagc cgaagaagaa aaagcacccg
    13801 aaaaaacaca tcttcaagcg gataaaaaaa ccgcttcgcc tgccgaccct cagcctgcca
    13861 gccccaaaaa gaaaatctcc tccccgtggc ttgcggttct gggcgggttt gtgctgcttg
    13921 gtttgtgcgg agcctgctgg ctgctcaacg ccagattcaa ctttttcggc ggaaccacca
    13981 accccacagc cacccaatct gtaaccctcg ccacagacgt acccaccgaa gaagttgtcg
    14041 aggaaattct ccccaccgaa accagccagc ctgccagcga accgaccgaa gcccccgcca
    14101 ccgaaatctc cctgcccgcc gaaatcaccg acgggaagaa tatctccatg cgcctagtcc
    14161 ccgccgggga attcatcatg ggcaatgacg atggcaatac cgaagaaagc cctgccagca
    14221 aggtccaggt cgctgccttt tacatggaca aatacgaagt caccaatgac atgtacgccg
    14281 aatgtgtggc agcaggtgta tgcaaactgc ctgaaaaacc agggtctgcc acgcgctctt
    14341 cgtattacaa caacccatcc tttgcaaatt atcccgtgct ctacgtcaac tggaccatgg
    14401 caaagaccta ctgcgaatgg cgggatgccc gcctgcccac cgaagcagaa tgggaaaagg
    14461 ccgcccgcgg catggatgat caacgcgcct acccatgggg taacgacttc aactgtaact
    14521 ttgccaacca ttcaggttgt gttggcgata cagcccccgt caaccaatac aacagcggac
    14581 aaagccccta cggcatttac ggcctctcag gcaacgtctg ggagtggacc agcagcctgt
    14641 ttgccttcta cccctacagc gccaccgatg ggcgtgaaga cccaactgca acaggtttcc
    14701 gttccgttcg cggcggttcg tggaatatct ttggcggctc aggcggaaac atccgcattg
    14761 acacgcgcta caaactcgac cccgtttact tcggcgccta cgtcggcttc cgttgcgcac
    14821 tgacaaaata gcagcaacac aacagccgcc caaaccaatc ccatttgacg accgatcgca
    14881 ggtgcgattt gtcgtcattt tggttaacac gcagacaaag tctgtctgtc atttcgctca
    14941 agccgccgtc ctcggcggcg ttctacgaat aaatcctccg ccgaggacgg cgaagggagc
    15001 aactcatcat tttggttaac gcatggtcac aatgtagtgg gaaacgcaca acaagtcgct
    15061 tataatagcg gcattcaacc cgcttaaaat ttgctatttt catccgagga aacctttatg
    15121 ccgttgctga tcggtcaaac atttgggcgt tatcaaatcc ttgagctgct tggagaaggg
    15181 ggcatggcca ctgtctacaa ggcatacgac acccatcttg agcgtgaagt agccatcaag
    15241 gtgatccgcc gcgatgcctt cccccccgac gacatgaaca tgctgctcaa gcgttttgag
    15301 cgtgaagcca aatcactggg caggctttcg catcccaaca tcgttggggt gattgactat
    15361 ggcgaacacg atggttctcc atatcttgtc atggtgtacc tgcccggcgg aacactccgt
    15421 gaacggcttg gaaaacccat cgcctggaga gccgcagtgg aattgatcct gcccattgca
    15481 cacgccctcg aatacgtcca cgaccgcaac atcatcaatc gcgatgttaa accctccaac
    15541 atcctcatga ccgaaaaagg ccagcccatg ctcaccgact ttgggttggt aaaacttttc
    15601 ggcgaacagg aaaaagaatc aacccacctc accagttcag gcaccggtct gggcacaccc
    15661 gattacatgg cccccgaaca gtggacaggt gaagccattc cacaatcgga cttgtattcg
    15721 cttggcgttg tactctacga aatgatcacc gggcgcaaac ccttcaccgc cgatacccct
    15781 gcaggcgttt tgctcaagca agccaccgaa gccctgcccc tgccgcgggc ctacatccca
    15841 gacctgccgc aggaggttga atcggttctg ctcaaagtcc ttgccaaaga ccctgaagat
    15901 cgctacccca acatgcacac cttcatcggt gaattgcaaa acctgctcgc tggcagggaa
    15961 gtctctgcca cttcgatcaa agccgaccag cttcgcaaaa agatgaccgg caggaccgaa
    16021 gcgaccacat ttgacccggc aacgggtcgg acccaaaagg cattccccac cctttttgcc
    16081 gtcctcggcg ggttgggcgc attgatggtc attgcggtca tcggcgtatt gatctacgct
    16141 gggaacggcg gcaccgccct gcctttcctt gccgcaccca cccaaacctc acaaccgacc
    16201 gcaattccct ccgctaccaa tacagccatt ccgccgacag aaacggtcga ggtcactgcc
    16261 gccccgacgg aaacgcttgt tccaacagaa acagccatcc cgtctgaaat aagagacgag
    16321 cgcaatgtcc cgatgatcta cattcccgct ggagaattca ccatgggcag caacgacagc
    16381 ggtgatgtcg cctcgaaacc cgagcacaaa gttgagcttg gggcattcta cattgacaaa
    16441 ttcgaagtga ccaatgaaag atatgatgcc tgcagatacg cagatgaatg tgtggcacca
    16501 cagcaactcg gaactgccac ccgaaacaac tactacacca acgctgtctt caaggattac
    16561 cccgtcatct acgtggattg gaaatgggct aaagcctatt gcgagtggag aggggcacgc
    16621 ctgcccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtg gattggaaat
    16741 gggctaaagc ctattgcgag tggagagggg cacgcctgcc cacggaagcc gaatgggaaa
    16801 aagctgcccg cggaactgat ggtcgtcttt atccatgggg taatgaaaag cctgattgca
    16861 acttcgccaa cattacaggc tgcgtgggtg atacaacccc ggtagaccaa catgaactgg
    16921 ggaaaagcgt gtacggcgtt tatggaatgg ctggcaatgt gatggaatgg accagctcac
    16981 tgttcgacta ttatccctac gatgccgctg atgggcggga aaaccctgac tctaccaaag
    17041 agcgcattgt ccgcggcggc gcatggaatc tcttcggcgg caacaacgga aacgtccgcg
    17101 tggatacgcg ggtgaaattg ccccctgaat attacggagc ctatgtcggc atccgctgtg
    17161 cgaaatcggc gcaataaaca gaagaactcg gaaccgtaaa agttccgagt tctttttatt
    17221 tccaacaaca cgggctggat cagctaaaaa tcccaggtgc gcgccactcg tacagcactt
    17281 cgagattgcc cttcaggaag gattctcgtg tggcgtttct ttgaattttt ccactcgtgg
    17341 tcttctggat ggttgatggc atgatcaata caaccgcctg cacacgcaga ccatgattct
    17401 tcgccaccgc catacgaatg gcattggcaa ttccctcaaa atcattgctc ttgcgatact
    17461 cgcgttttac ttcctgcacg accacaagat gatccacctg attcgcctcg accgcaaagg
    17521 cggcgccaaa gctggggcgc agggcatcat ggcatttttc catcgtcaac tcaatatcct
    17581 gcggataaaa attacgccct tgaataatga ccatatcctt gatgcggcca gtaatgtgga
    17641 tctggtcgtt gtagataaaa cctagatcac ccgtccgcat gaacgggcct tcgttggacg
    17701 atgcaatata agcctgaaat gtcttttcgg tttcttcggg gcgcttccaa tatccctgtg
    17761 ccacactggt gctgctgacc cagatctcgc caacctcgtc tttcgcacac aattcctttg
    17821 tctgcgcgtt cacgattcgg atatctgcat taatggaact ccatccgcag cccacgctnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ctgcaaatca cggactgtct
    18001 ctatcaggtt gaagtgtgtg atcatcacac ccttgggcgt gctggtggag ccagaggtgt
    18061 attgaaagaa agccaggtca tcggctttgt tttcaacttc ctgccattgc gattcaacac
    18121 ccgcttcgag cttttcattg accacccatt tcaggtcatg gaattcggga tattcatcga
    18181 ataactgttt gagcttatcg aacaattcca tctgtgtgca ggccactaca gcatcggcat
    18241 cacttagcat ggccctgaac ctgggcgtgg cacgtttcag gtgcggaaca atggtcggca
    18301 cagcgacgac gcccgcatac aagcacccga aaaaagcgac gatgaattcc agacctgatg
    18361 ggtacagcaa caaggcgcgg tcacctggtt tggtgtgctg ctgcaaagtg gcggcaaatg
    18421 cccgtgcctg gcgatcaagc tcggagaagg tcagaggcgc ttcctgatcc tccccatcga
    18481 caaggtacat gtaggccagt tcattcccga ttcgatccgc ataaaagcgg accacctcca
    18541 caagactgac agcctcttca gggttttgga tcatcccatt ttctccttaa tcctggcggc
    18601 catggcatcg atggtgttca tgttgtcgac cgtcaactca gggtcgggaa tatagatctg
    18661 gaactcgatc tcgacaaaca ccccaacctg cgcgagcgaa aacgagtcga tcaacccgct
    18721 ggtgatcagc ggctcatcaa acttaagcgg ataatcggca tcgttcatga tctcggtgca
    18781 gatgaatttt ttcagttttt ccttgatctc gatttcttcc atgttagaac tcctgataaa
    18841 tgaatggagc cactgccgcc tgccgcgtgg acaggaacaa aagcagaagc cctaccgtaa
    18901 gcaagaacac ctttacaggt tttgacagac gcagcaagcc caattcgtct ttggctggca
    18961 attgcatcac atcaatgaaa aaggacaggc cgattgccag aatgggcttg atgatctgat
    19021 gaacggacag cgtctgaatg ataccccatc tgaacaggcc gctccaaaat gccagtgtct
    19081 ggctgaaatc cattttgaaa ggggcgttga tcaggatcaa cacaagcaga gtcactgaaa
    19141 tgctgacagc ccgtctccag cgcggcggct gcacagtggt ctttttgaca aacgtttttt
    19201 gcacacgttc atatccctgc agcaaaccgt tgagcgcacc ccacaaaatg aaatgcggag
    19261 ccgccccatg ccagatgccg ctgacgatca tcgtgaccag cggcggaacg accaggttgg
    19321 gccaatatcg tccgctggga ttgcggcgca gcatggcacg gctgacaggc aggtaaatgt
    19381 aatcgcgcag ccaattggaa agggaaatgt gccagcgatt ccaaaagtca atgtaactgg
    19441 tggagaagaa cggctgctca aagttcggcg aaagctgaat cccgaacaat ccgctgactc
    19501 cccttgcgat gctggtgtag cctgcaaaat cattgtagat gacgaaggca aaggtcagcc
    19561 accagaaaag cagatcgggc atggaatatt tttgcggagc cgcaaataca cccaggggaa
    19621 tcatccgcac gatggtttca gagatcacca atttacggac gagaccgacg aagatcttga
    19681 taaaactttc aacaagctgg tcgttcccaa tttcaacatc ggcctgcaat tgcttgaaga
    19741 agttttgtgc ccgttcaatg ggacccgcca gcagtttggg aaaatagccg agatacagcg
    19801 agacttccac aaaactatga tttgcaggga tttgcttgcg gtacacatcc accaaatacg
    19861 aaatggcctg cagggtataa aacgaaatgc cgatgggttg cagcaggtca agggagagca
    19921 attcgtttgt cagaccgatc cgcagcagag ccttgaaaaa atagaccgag gtctggtcga
    19981 agtatttgta aaacagcaaa acgcccagat tcgcggcaac accgatccat acccaacgag
    20041 tcttggcggt gacttctgct ttatccagtt tgagcgcaac ccaataattg acaatggatg
    20101 aagccaggag aatggcagcg aaggtccaat cccaggtgaa gcagaagaga tacgaggcga
    20161 tcagcagcac atggttctgg gcttttttag ggacgaggta atagacccca agcgcagcca
    20221 ggacaaagaa aaagaaaccg ttggaggtga tggaaagatt cgaaaaaagg tcagtcattg
    20281 ggttattcat tgctcaagcc gctgtcccag cagcgaggac gccgcgggag catagcatga
    20341 gggcatcaag gtatttgccc tgaaatggga atttgaatgc tgctcaaatg ctcgccaagc
    20401 aggcggctga aatattcgct cccaactttg ttgaaatgga caatatcgta ccagccatct
    20461 gttgggatct gaacagtctc ctgtgtcagc cacagatcgg tgtggacata ggctgcttca
    20521 ttttccagca tgttgcggaa atcgggatac accttgcggt tgtacgaaaa ctctttgagg
    20581 gtgaaaaaga tggggtcaac aggtgtttcg aacaggacga ttttcgtctc cgcgctgttc
    20641 agggccagca attcgtgcaa gccatcgagt tgttcgccag taatttctgg tctgtccaac
    20701 agactgtcaa agtaatccac ctgctcctgc tgggacatgt tcgtcttttc agagaatttc
    20761 agggagtgac cgttgagccc gatgtggtct gcgttctctt cgccgttgag caggtaccga
    20821 tccgcgccga ggtagaagcg ataggaattc gagttttcga tgagccagcc atcggccgaa
    20881 aaatcatcca attgatattt cacccacggc gaggacatga tgcttgaatc tgacccagcc
    20941 tgtacggcgg tgtaatcgaa caagtctgtc ccaaaaacga tgatggctgg atggaaattc
    21001 ttgatgagca attttgccaa agggtaggct gtgatcggct ttagcccgcc cagcccaaag
    21061 ttttgacaat gaatcgtgtt ccccgtcgag tcaaaatatg cctgctccac gatcgtcggg
    21121 tcaatgccgt aaagcacctg cgaatcgccg aggatgatgc aatcgatctg cccatcctga
    21181 acttcacg
//
LOCUS       DAOQLC010000074        21146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_867_length_21146_cov_21.249348, whole genome shotgun sequence.
ACCESSION   DAOQLC010000074 DAOQLC010000000
VERSION     DAOQLC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_21146_cov_21.249348"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PL000_13370"
     CDS             <1..696
                     /locus_tag="PL000_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89939.1"
                     /translation="PSGHMRTNENGSDGTKGCKKKSYCEYAQEKSDAEIEARQAAKEA
                     AKAAKEAAKNGSSTETPTVTGTPTATSTPTIIPTSCIPRPGCTTNTPTPTSTPSSTLT
                     PTYPPTHDAFIFGVSGSFDDGMVSGSSHSAGFEYLYAFDDGSSGLFRTHGYGTSMGES
                     AQAGFYVGRAWGITTAAENVSSSSPSVALTILSAQFTYTGDPWSGSFTVGLGAPGGVS
                     IAYSDNQTVPAFP"
     gene            708..1316
                     /locus_tag="PL000_13375"
     CDS             708..1316
                     /locus_tag="PL000_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89940.1"
                     /translation="MKKTNKKISSKTISILLPLSILIFVVLNVFNVINSTFPHGLGLS
                     DELIRYQSPSAPYSMLVSEKWTILDAPIENYEYTDVRTSIGSPYASLKIYVSRKSFET
                     DTLNDVVDWGRATAGYQLLGRKEEDVSEYSNKKRKGILDEYIIDISPFIKLTWNSKYN
                     HCFDYYFLEKGYGYKFSFCSVEKVWNNAKDLFIKLIDSIEIG"
     gene            1461..1865
                     /locus_tag="PL000_13380"
     CDS             1461..1865
                     /locus_tag="PL000_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89941.1"
                     /translation="MDSSQTADASYQYAENRITSIIKLASGTCETPVLFASKWDFTYD
                     GDGTRTATLITPYNELGEPQSAQLTAYFFGGAYETQADGTIRKYYSLEGQTILRTLTT
                     GSSTLSYFLTDHLGLGRRHRRCQRSNVTSPNS"
     gene            1975..2169
                     /locus_tag="PL000_13385"
     CDS             1975..2169
                     /locus_tag="PL000_13385"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC89942.1"
                     /translation="MTTQRQALRRSRSNRVVAGVCGGLAEFFGVSTFWFRLAFLIALI
                     PGGVPGILVYLAMVFIVPSE"
     gene            2352..3578
                     /locus_tag="PL000_13390"
     CDS             2352..3578
                     /locus_tag="PL000_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89943.1"
                     /translation="MAETPNFDKASLLSLAFKGLAENDLREMATLTRLCTYPADHILC
                     REGAYEEIFYIIADGNAVITKQISEEEGERVLRIAGKGDLVGEMALIQNSPRSATVRA
                     TTELTVLEMGKKDFETMLSRSPSMAINIIRTTLDRILENDQMAILDLQKNNKVLRQLD
                     RNKMEFIQVAAHELRTPLTIIKGYVNVLGSFPELQENSALKEVMEGIVKGSERMHEVV
                     NTMLDVTRIENEQLRISPAPVPLKRILHELSSDFRKAAEDRNIEIIVEQDEDTPAINA
                     DPSLIQKALYHLIVNAIKFTPDGGKITLRSYPVLMENHAPGVELSIQDTGIGLDAEHH
                     ELVFEKFYQVGNVSIHSSGKTSFKGGGPGAGLAIVRGVARAHGGKVWVESKGHDEVNF
                     PGCTFHLQLPIDPPRH"
     gene            complement(3614..3952)
                     /locus_tag="PL000_13395"
     CDS             complement(3614..3952)
                     /locus_tag="PL000_13395"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HNC89944.1"
                     /translation="MAPTNSKTVWSSEEGDLRKKEAASASSHSLPPQQQTIYLHRESK
                     GRGGKGVTLVKGLVLSEADLTALAKKLKQACGSGGTIKDGVIEIQGEQREKIAEVLRS
                     LGYKVKIAGG"
     gene            4179..5030
                     /locus_tag="PL000_13400"
     CDS             4179..5030
                     /locus_tag="PL000_13400"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNC89945.1"
                     /translation="MTAYIFAWYLFLFIPLLFFAWATRRKWLGLTVTSSILIIVFTYL
                     PLYLPNRPASSAEGGFSVKIMSYNLHGIPEIDGIVEVIRRENPDVLLVQECSPALCSP
                     LYHGLDDLYPYKDMVIKDFGQAAYSRYPMKRTSAEANQGRTQKLIIKAPQGPAAVWNI
                     HPIPPFLIPPQEYDAQMSALVADIAREKGPLIVAGDLNATDQSEAYRKINQYLIDGYW
                     ETGWGVGFSYPAPPYTFMDSGLQMGPIWRIDHIFHSSEFTATDAYIVKDAGGSDHFPI
                     VVVLSLK"
     gene            complement(5062..5463)
                     /locus_tag="PL000_13405"
     CDS             complement(5062..5463)
                     /locus_tag="PL000_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89946.1"
                     /translation="MTKIMIVDDDPEATSILSSLLKIEGYIPISVNDSTLAIQKANAV
                     QPDLFLLDLMMPVLDGFKLCRLLREERIFLRTPIIIVTALSSADSRVVAFGAGANDYI
                     TKPYLPHELVLKIREQLERARAGLIDPFDGL"
     gene            complement(5508..7904)
                     /locus_tag="PL000_13410"
     CDS             complement(5508..7904)
                     /locus_tag="PL000_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC89947.1"
                     /translation="MRTLPKSKWMRFLLVVIFLVGGYFISVFYDLPSVHAIPESLNQP
                     SVRITDRNGRLLYELIPEVGGRNTVLAVENIPQCMKDATIAVEDGNFYSNPGVDLTGI
                     VRALWINLSGGETLAGGSTITQQIARTLLLKDEVTERTVRRKLRETVLAWQLTQTYSK
                     DEILALYLNQIYYGGMAYGIEAAAQTYFGKPAVDLLLPECALLAGLPQTPGIYNPFTN
                     PDLALDRQKVVLGLMEKEGFITATQRIDAENSPLSYNAEPYPIEAPHFIWMIKDQLDE
                     LYLSGQLSVEQSLIIRTTLDLDMQNLAEEIVKRRIELFKPLEGQTNFNVKNAALVVLD
                     PHTGEILTLVGSADYFDVSINGALNMAITRRQSGSAFKPIIYAAALDPTRMDAITAAT
                     PILDVATTFTTKDGQPYKPVNYDGREHGFVPVRQALASSLNVPAVLTLQNVGIENAIS
                     LANQLGIRSLSDPRNYDLSLALGGGSMNLLELSGAYAALANEGNVTGLFSVLEIRDAD
                     GNLLYEPERPAAHRVVDARVAWLISDILSDDQSRALGFGINSVLKIDRTAAVKTGTTT
                     NFHDNWTIGYTPDLLVGVWVGNSNQEAMHNVTGLTGAAPIWHETMRSILQGRPDKMFS
                     QPAGLTQINVCSLSGLLPTEACQYTKNEWFIEGTQPTAYDTFYQRLDTGQVVLSLPVE
                     AQAWARSQGLPLLDLANEPNFEADFLTLTSPTDHTTYRITPNLDLSAQQLAFSAPTVP
                     GLTQMNFFVDGASFATLNSPPYQVWWPLSLGQHRFWVEGIMSTGELVKSTVVTITVVE
                     "
     gene            complement(7999..9333)
                     /locus_tag="PL000_13415"
     CDS             complement(7999..9333)
                     /locus_tag="PL000_13415"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HNC89948.1"
                     /translation="MNNIYDILEICLQELENGADMDALLARYPSFAGELRPILKASVE
                     ARTMAQEPSPEVVRRGRAKLLQHAAEMREAKLAPRKRVIPFFQRMAISFTMTALFLAS
                     GTGLVGASSSALPGENLYPVKLTWENVRLFFTFDHAAREVLEHAFGNERLHEISELLA
                     EGRHETIQFSGIFMDVNGLTYVSGIQVAILDTTNLPVDGLVNGAAVTVTGRTNAAGFV
                     DAASIILLPPGTVVPTGEPVKVEDNQNENINLNGNDNSGSEENENNGNDNVGVNENEN
                     ANENINDNENGNGNDNGNDNGDNNNDGGGDDDGNDNGGTDNENGNDNDGGDNGNDNAN
                     ENSNENENANENTNENDNGGSNDNDNVNDGVNDNTNDNSNDNTNDNVNDNSNDNDNSN
                     DNTNDDNNNDDNSNDDANSNDDDNTNSNGGSDPINARYVEAFLSRKDFTLNL"
     gene            complement(9326..9874)
                     /locus_tag="PL000_13420"
     CDS             complement(9326..9874)
                     /locus_tag="PL000_13420"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC89949.1"
                     /translation="MPPNDLQKELDGLQKLDAQTIGAVYDQYFSEVYRYVRYRIGDSN
                     AAEDIASDVFVRLLEAVQNGQPPKTSLKGWLIGTASHLVTDHLRRKYRRPENELHESL
                     PDSGANVPSEVDQREQNQAVNNAYAQLTPEQQNVLALRFGQGYSLDETAAFMNKNVNA
                     VKALQFRALAALQREVGEVNYE"
     gene            complement(10061..11566)
                     /locus_tag="PL000_13425"
     CDS             complement(10061..11566)
                     /locus_tag="PL000_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89950.1"
                     /translation="MQKQTISKFLPLLLFVLFFIGMAAIQFATSDMPDNDGFYHIKLA
                     WLMRTEGLKPDFVWLPLSILSEKEFYDHHFLFHVALIPFTFGDLHVGAKWAAVTFSSL
                     AFLAVWYLFHRQRIPLAWLWALGLLGISDAFLYRMSITRAQSLSLGILALALAWILEG
                     KYKHLAVLSFIYVWAYDAFPLMMSLTVLHLLAVAMIERRLEYRLLLWSGIGVVLGVII
                     NPYFPANLVFTYRHLLPKLVEATSVSVGNEWYPYETAQLLENSLPALVAFVSGALALG
                     LTGRKMDVRTATGFLIALLFGLMLFQARRFVEYFPPFALIFAAFAWAPLFVDPEPASG
                     FQSRFSRSKWDELMANMPGLLLMLAVVVGMVKSIPAVQKQMQRSKSYELYADASAWLV
                     EHTAEGERVFQTDWDDFPRLFFYNTHNTYLIGLDPTYLQLYDAEMYDTWVSITRGKTP
                     NPSEIIATQFEARYVHSDLNHENFLRVAAEDPGLKEVYRDGQAVIFEVITP"
     assembly_gap    11731..11830
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11831..12483)
                     /locus_tag="PL000_13430"
     CDS             complement(<11831..12483)
                     /locus_tag="PL000_13430"
                     /inference="COORDINATES: protein motif:HMM:NF039822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HNC89951.1"
                     /translation="MKQFFRYLRPVLVLLALVMNACASAPVATTTDTGAAKVEASGVE
                     FTGVIESIDGNTWVVNGQTITVDPALVQGTYAVGDTVKIEASVAEDGSIVVTRLEAPS
                     SSDDNTNTGSNDNTSTGDNTNTGTGDNTNTGSGDNTNTGTGDNTNSGTDDNSNDSNSN
                     DDTDDDGDDNSNDGTDDNSNDGTDDNSNDGTDDNSNDDNSNDDGSDDNGNDDNSNDDG
                     "
     gene            complement(12617..13729)
                     /locus_tag="PL000_13435"
     CDS             complement(12617..13729)
                     /locus_tag="PL000_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNC89952.1"
                     /translation="MKKTVSLLFILILLTACTTTSEANPTPPVVGTVIESDFTNPLTV
                     VELGAGYGVRGDWFELYFTDPSSPLAAQETGGPDGPLVNAIDNARLSVDVAIYSLSLN
                     SVRDALLRAHDRGVRVRMVMESDNLDRSDPQKLKDAGIPILGDRREGLMHNKFVVIDN
                     LEVWLGSMNFTDSGAYKDNNNLMKIRSTKLAEDFTAEFEEMFVDDIFGPDLGNPTPNP
                     RVTIDGTNIDVYFAPEDHAQAALFDLVDSVAQASIYFMAYSFTADPIGNAIRSASAEG
                     LTVAGVMDADQIKSNLGTEYDPFKQAGLEVYEDNIPGLMHHKVMIIDDSIVVFGSYNF
                     TNSAETNNDETLLVIYNADIAAQFKAEFFRVFRSAQ"
     gene            13766..>15327
                     /gene="murJ"
                     /locus_tag="PL000_13440"
     CDS             13766..>15327
                     /gene="murJ"
                     /locus_tag="PL000_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC89953.1"
                     /translation="MSETNGNANRQIARAAGTVMAAILLGQLAGLARGVIVARAFGAS
                     PELDAFFAANRVSETLFLLVAGGALGSAFIPMFTGLLAKNENDSAWRLASAIGNAVTL
                     TLSLLAVLVAVFAPQVVRYALAPGLSSDPQLFALTISLLRIQLISAVLFGIGGLIVGI
                     LNAHQVFLIPALTPAFYQLGIIFGALVLAPTLGIYGLAWGVVIGSVLYVALQTPTLAK
                     LAARQSQLATFHFSLGLHDANVRQVLMLMGPRLIGVAVVQLNFWVNTWLASQMASGSV
                     TGLYYGFSLMLMAQAAIAQSVAIAAMPTFSAQHALGQTDEMRASLASSLRGVILLALP
                     ASIGLMLLRQPLISMLYQRGKFDATDVQIVAWALLWYAAGIVGHSIMEILTRAFYAQH
                     DTRTPVIIGTIAMGLNVAFSILFAKLFTAVGWMPHGGLALANSFATALEATALFIVMR
                     KRLQGIEGRHILGGALPSTAAAVVMAFSIIGWLSAGKNLNAWILALIGVTIGGGVYFA
                     MLWILRVPELQY"
     assembly_gap    15328..15427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15497..15949)
                     /locus_tag="PL000_13445"
     CDS             complement(15497..15949)
                     /locus_tag="PL000_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HNC89954.1"
                     /translation="MNLAALLDNKVLIAVLAGWLIAQALKIPTEYFRSRRWMWAMFFA
                     AGGMPSSHSALMVSGTIAVGLYHGFDNPLFGLAVAVTMIIAHDAAGVRRQAGMHAERI
                     NVLFEELLKGHMWDENELKEVIGHTPLEVVGGILLGFFVAIMQWIIWP"
     gene            complement(15946..16188)
                     /gene="xseB"
                     /locus_tag="PL000_13450"
     CDS             complement(15946..16188)
                     /gene="xseB"
                     /locus_tag="PL000_13450"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNC89955.1"
                     /translation="MAKSKSSKSIEELSYEEALAELEQIVSTLEDEQNPLEDSMKLFE
                     RGQALVRQCGALLESAQLKVQKLAGESLIEFEEESE"
     gene            complement(16217..17422)
                     /gene="xseA"
                     /locus_tag="PL000_13455"
     CDS             complement(16217..17422)
                     /gene="xseA"
                     /locus_tag="PL000_13455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNC89956.1"
                     /translation="MQPTLFSSVHLTVSQLTFHIRKQLEDDPTLQDVWVDGEISNLSK
                     PASGHIYFTLKDKNASLRCVIWKLDAGRLRVNLQDGMAVEVHGRITVYEPQGQYQLIV
                     NLVQPKGEGRLFQEYMRLKAMLESEGLFAADRKRLIPDRPQTIGIVTSATGAALRDML
                     NTLRRRQPLARVILAPSPVQGVEAPPALVNALNSLNRQKPDVILLARGGGSIEDLWAF
                     NEEQVVRAVAASKAPVICGVGHETDFTLCDFAADLRAPTPTAAAELATQTTLDDLHFQ
                     LSTISTRLTDLTSNLLAEQKAGLSVLAGRLKYTSPERRIQSEYQRLDELSRRAWSAID
                     HRIQLQASLVDGMSKRLQSLNPMGILARGYAIITRKVDGRVVGKVADADSEMKVRVSD
                     GEFDVNRKS"
     gene            17535..18023
                     /locus_tag="PL000_13460"
     CDS             17535..18023
                     /locus_tag="PL000_13460"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNC89957.1"
                     /translation="MNHLWSPWRMSYIENHTKENGCVFCIAQAKADSEENLIAFRGKN
                     AYVILNRFPYTSGHLMVIPFSHTSNLEELDAETRAEMMELASRCTTVLRNEYRTQSFN
                     MGINIGEAAGAGVLGHVHIHIVPRWVGDTNFMSSVGETRVLPETLEATYKRVRDAFQT
                     NP"
     gene            18025..18699
                     /locus_tag="PL000_13465"
     CDS             18025..18699
                     /locus_tag="PL000_13465"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC89958.1"
                     /translation="MHKYSLALKSLFEKNANPAQAGPMQKYMRDQFEYLGIKTPQSAA
                     LMKGFVAEHGLPPLEKLDVIVRDLWSLPQREFQYAATGLIGKLEKKVEPEFITTIEYL
                     LVTKSWWDTVDTLASHAVGSQFKRFPEIRKTYLKKWRKSENFWLRRTTLLFQLGYKKE
                     TDFDLLCEIVRENLGSDEFFINKAIGWALRQYAHTNPSPVKKFVAATKELHPLSRREA
                     LKNIEE"
     gene            18769..19017
                     /locus_tag="PL000_13470"
     CDS             18769..19017
                     /locus_tag="PL000_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC89959.1"
                     /translation="MNSVWQIQDAKNKLSEVIARALQQGPQLITKHGEKTVVVISYIE
                     YEKLRKSQGKISEFFSASPLAGINLARDKSIPRKGLEL"
     gene            19014..19430
                     /locus_tag="PL000_13475"
     CDS             19014..19430
                     /locus_tag="PL000_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC89960.1"
                     /translation="MKYLLDTCVISELIAKTPNPNVVEFVDAIETDNIFLSAITIGEI
                     AKGIEKLPESKRKRELQTWLKEDLLVRFDDKVLPLDTEVLIQWGILSARLEGTGITLP
                     SMDSLIAATALTHNLTLITRNEGDFQNTGIEIINPW"
     gene            complement(19462..19854)
                     /locus_tag="PL000_13480"
     CDS             complement(19462..19854)
                     /locus_tag="PL000_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HNC89961.1"
                     /translation="MNELNSLYDLLGGEVGVRALVERFYDIMDSSPEAKDIRALHAAS
                     LKQSREKLFLFLTGWSGGPPLYIEKYGHPRLRQRHMPFSIGGIERDQWLWCMNQAVEE
                     MNILPAVKEHLRTRFAEVADFMRNREEG"
     gene            19957..>21146
                     /locus_tag="PL000_13485"
     CDS             19957..>21146
                     /locus_tag="PL000_13485"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC89962.1"
                     /translation="MSKENDAVILSAARTAVGKFQGGLSGFSATKLGAIAVQAAVERA
                     GIDPQNIEEVLMGNVVQAGNGQAPARQAAIFAGVPATVSATAINKVCGSGLKAAMMSA
                     QAIRAGDGDLFVAGGMESMSRAPYLVSGRSGELKFGNQALTDALLNDGLWDPFENWGM
                     GMAAEFIADEYEVTRAAMDEFALRSHLKAVEAQEKGMFSAEIVPVQIPGRKGEVTIFD
                     KDEGPRKDTSLETLAKLKPAFKADGKVTAGNASSMNDGAAAVVISSRAYAEKNNLKPL
                     ARIAGYAQAALEPKYLFAAPAHAIPKLLKKIDWKLSDVDLIEVNEAFAAQVLADGYAL
                     ADQGWDWDKVNVNGGAIAIGHPLGASGARVLTTLIYALKNRGLKRGVASLCLGGGEAV
                     AMAV"
BASE COUNT         5336 a         5375 c         5205 g         5030 t
ORIGIN      
        1 ccctcagggc atatgcggac caatgaaaat ggcagcgatg gtacaaaagg ttgtaaaaag
       61 aagtcttatt gcgagtatgc ccaagagaaa tcagatgcag agatagaagc tcgacaagca
      121 gccaaggaag cagctaaagc agccaaagaa gcggcgaaaa atggtagtag cactgaaacc
      181 cctactgtga ccggaactcc cactgcaaca agtactccta ctattatccc gactagttgt
      241 ataccccgtc ccgggtgtac aacaaataca cctacaccta cttcgacacc ttcaagcacc
      301 cttaccccca catacccacc tactcatgat gccttcatat ttggagtaag cggttcattt
      361 gatgacggta tggttagcgg cagctcacat tcggctggtt ttgaatattt atatgcattt
      421 gatgatggct catctggttt gtttagaact catggctacg gaacatcaat gggagaatcc
      481 gcacaggccg gtttctacgt gggaagagct tggggtatta ctactgctgc tgaaaatgtt
      541 agcagctctt cgccatcagt tgccctcacc attctttcag cgcaatttac ttatacaggg
      601 gatccatggt cagggagttt tactgttggc cttggcgccc caggtggagt ttctatagcc
      661 tatagtgaca atcaaacagt gccagctttc ccataggaga cggcttcatg aaaaagacaa
      721 acaaaaaaat atcttcaaaa accatttcga tactgctacc cttgtcaatc ttaatatttg
      781 tagtcttaaa tgtgtttaac gtaataaatt caactttccc tcatggactt ggtttatccg
      841 atgagctaat tcggtaccaa agccctagtg ccccgtattc aatgctggta tcagaaaaat
      901 ggacaattct tgacgcaccc attgaaaatt acgaatacac tgacgtaaga acaagcatag
      961 gaagtcctta tgcatcattg aaaatatacg tgtcaaggaa gtcatttgag acagatacat
     1021 taaacgatgt tgtcgattgg ggaagggcga cagccgggta tcaattgtta gggcgtaagg
     1081 aagaagatgt ttctgagtac tcgaacaaaa aacgaaaagg aattctggat gaatacatta
     1141 tagatatttc gccatttata aaattgactt ggaacagtaa atataaccat tgttttgatt
     1201 attatttcct agaaaagggt tatggttata aattttcctt ttgtagtgtc gaaaaggtat
     1261 ggaacaacgc gaaagatttg tttataaaat taattgatag tatagagatt gggtgatggg
     1321 tcaaaataaa tgataatgtc cgtagcgcga gataatatct cgcgctacat catttacaca
     1381 aacccattac cagagattaa tgatgatata gagtagacct cctacgcacc cctacttccg
     1441 cgcctcacgc tgtcacctcg gtggatagca gccagaccgc agacgccagt tatcaatacg
     1501 cagagaatcg catcaccagc atcatcaaac tcgccagcgg aacttgcgaa actcccgttc
     1561 tattcgcctc caaatgggac ttcacctacg acggcgacgg cacacgcaca gccacgctca
     1621 tcacacctta caacgaactc ggcgaaccgc aatcggcgca gttgaccgcc tacttcttcg
     1681 gcggcgcata cgagacccag gccgatggta caatccgcaa gtattactcg ctcgaagggc
     1741 agacgatcct ccgcacgctg acaacgggtt cttcgaccct gtcctatttc ctcaccgatc
     1801 atcttgggct cggtcgtcgc catcgccgat gccagcggag caatgtgacc agtcccaact
     1861 cataggcggg gttgacagac ctctcataca caggtcaaca gaccctgaat cgatgcatcc
     1921 aaattccaaa acgcgcgtat atccctgtaa catcaaatga atttggaggt tcaaatgact
     1981 actcaaagac aagcattacg acgttcccgc agtaatcgcg tggtcgccgg ggtatgcggc
     2041 ggattggctg aatttttcgg ggtgagcacc ttctggttcc gcctcgcgtt cctcatcgcc
     2101 ctgatccctg gcggcgtacc cggtatcctc gtatatcttg ccatggtctt catcgtacca
     2161 agcgaataag tgaaccgtgg aaatttcaaa actgccgaag ccaggctggc ttcggcagtt
     2221 ttgattaaac tcgtccgtgc cgcaaaagga ttaaaagaca gccacctgca aaaaattaag
     2281 tagaatccac atgcaagatt tcaatcgaat agcaatagtc agggttactt gccagggtta
     2341 tacttgttcc aatggccgaa acccctaact tcgacaaagc atccctgctg agccttgcat
     2401 tcaaaggcct cgcagaaaat gacctgcggg aaatggcaac gctgacgcgg ctttgcacct
     2461 atcctgccga ccatattctt tgccgcgagg gcgcctacga agagatcttc tatatcattg
     2521 ccgatggcaa tgcggtcatc accaaacaga tcagcgaaga agaaggcgaa cgtgtcttgc
     2581 gcattgccgg caaaggcgat ttggtgggcg aaatggcgtt gatccaaaat tcgccgcgct
     2641 ctgccaccgt tcgcgccacc actgaattga ccgtgctcga aatggggaaa aaagattttg
     2701 agaccatgct cagccgcagc cccagcatgg cgatcaacat catccgcacc acactggacc
     2761 gaattcttga aaacgaccaa atggccattc tcgaccttca aaagaacaac aaggtcctgc
     2821 gccagttgga ccgcaacaaa atggaattca ttcaggttgc cgcacatgaa ctacgcactc
     2881 cgctgaccat tatcaaaggg tatgtcaatg tgctgggctc cttcccggaa ctgcaggaaa
     2941 actccgcatt gaaagaggtc atggagggaa tcgtcaaagg ctccgaacgg atgcacgaag
     3001 tggtcaacac catgctggat gtcacccgca tcgaaaatga acaactgcgc atcagccccg
     3061 cccccgtgcc attgaagcgg attctgcacg aactttcaag cgattttcgc aaagccgccg
     3121 aagaccgcaa catcgagatc atcgtcgaac aggatgaaga tacacccgcc atcaatgccg
     3181 acccatcctt gatccaaaag gctctgtacc atctcatcgt caatgcgatc aaattcacac
     3241 ccgacggcgg gaaaatcact ctgcggtcat atcctgtgct gatggaaaac cacgcccccg
     3301 gggttgaact cagcatacaa gatactggca tcggtctgga tgccgaacat catgagcttg
     3361 tcttcgagaa gttttatcag gtcgggaatg tatccattca ctcttcgggg aagacatctt
     3421 tcaaaggcgg cggaccaggc gcagggttgg ccattgtccg cggcgtggca agggcacatg
     3481 gcggcaaagt atgggtggag agcaaaggcc acgatgaagt caatttcccc ggatgtacgt
     3541 tccatctaca gctgccaatt gacccgccca ggcattaagc ggactaaaag tccacactct
     3601 ggtgatgtaa aaatcaccct ccagcgatct ttactttata gcccaaggaa cgcaacactt
     3661 cggcgatctt ttcacgttgc tcgccctgaa tttcaatcac cccatctttg atggtgccgc
     3721 cgctgccgca ggcctgtttg agtttcttcg ccaatgccgt caggtcagcc tcggaaagta
     3781 ccagaccttt gaccagggtc acaccctttc caccgcgccc ttttgattct cggtgcagat
     3841 aaatggtctg ctgttggggc ggcagggaat gggagctggc agaagccgct tcttttttac
     3901 gaaggtctcc ctcttcggaa gaccagacag tttttgaatt tgtaggtgcc atggcgcaag
     3961 gatacagtgg ttttctggca aaagcaagag gggtacaata aacaaaaaac agagggccag
     4021 ttatgtcaaa caatttcaca aaatcaggtc tcaaattgaa aactatattc tccgctgctc
     4081 gtaatgtatt tgagggggtg gtctggctcc ccagcattgg cctgtggata atgtatccag
     4141 ccagatggct gacaggagac ttgcttttga gcgtcagaat gacggcgtac atctttgcat
     4201 ggtacctatt tctatttatt cccctgctat tttttgcatg ggcgacgcgc cgcaaatggc
     4261 tggggctgac agtgacaagc tccattctta tcattgtctt cacttatctt ccgctctacc
     4321 tgccaaaccg cccagcttct tctgcagaag gtggattttc agtaaagatc atgagctaca
     4381 acttgcacgg tattccagaa attgacggga ttgtggaagt cattcgccgt gaaaaccccg
     4441 acgtcttgct tgttcaggaa tgctcccctg cattgtgctc accgctatat catgggctgg
     4501 acgatctata tccatacaaa gatatggtga tcaaagattt cgggcaggct gcctacagcc
     4561 gatacccaat gaaacggacc agcgctgaag caaaccaggg acggactcag aaactcatca
     4621 tcaaagcacc gcaggggccc gccgcagtct ggaacattca cccgatccca ccatttctta
     4681 ttccaccgca agaatacgat gcgcagatgt ccgcccttgt cgcagatatt gcgcgggaaa
     4741 aaggtccgct cattgtagca ggagacttaa acgccacaga tcaatcggaa gcgtaccgca
     4801 aaatcaatca atacttgatc gatggctatt gggaaacagg ctggggagtt gggtttagtt
     4861 accctgcccc gccatataca ttcatggatt cagggcttca aatggggccg atctggcgaa
     4921 ttgaccatat ttttcacagt tcagaattta ccgccacaga tgcctacatt gtgaaggatg
     4981 ctggcggctc agatcatttc ccaattgtcg tagtgctatc tttgaaataa acccctctgt
     5041 tgtgttgatc cgttggcaca cctacaaccc atcaaatggg tcgatcagcc ctgccctggc
     5101 acgttccaat tgttcacgaa tcttgagtac aagttcatgt gggagataag gtttggtgat
     5161 gtagtcgttg gcgcctgcgc caaaagccac caccctgcta tcagcgctgc ttaaagcagt
     5221 gacaatgatg atcggcgtac gcaggaaaat ccgctcttcg cgcagcaatc tgcatagttt
     5281 gaagccgtca agcacaggca tcatcaaatc cagcaggaac agatcgggct gaaccgcatt
     5341 ggccttttga atggcaagcg tgctgtcatt cactgagatc gggatatatc cttcgatttt
     5401 cagcaggctg gaaagaatac ttgtggcttc ggggtcgtca tcgacgatca ttattttagt
     5461 cataagtttt cgaattatac cgccttgaag acaagagtcc aaaaagatta ttccaccacg
     5521 gtgatggtca cgacggtgct tttcactagc tcacccgtag acatgatccc ttcgacccaa
     5581 aaccgatgct gccccaaaga caagggccac cagacttgat aaggcggaga attcaacgtt
     5641 gcgaaagaag caccatctac aaaaaagttc atttgagtga gaccgggaac cgtcggcgca
     5701 gaaaaggcca gttgttgggc actcaggtcg aggttagggg tgatgcggta tgtcgtatga
     5761 tcggttggag atgtgagggt cagaaaatcg gcttcgaaat tcggctcatt ggccaaatcc
     5821 aacaaaggca gtccttgtga acgtgcccag gcttgagctt cgacaggcag ggataaaacg
     5881 acctgccccg tatccaaacg ttgataaaaa gtatcgtagg ccgtgggttg tgtgccttca
     5941 atgaaccatt cgttttttgt gtactgacag gcctcggtgg gcagcagacc cgagaggctg
     6001 catacattga tttgagtgag accagcaggc tgtgaaaaca ttttatcggg gcggccctgc
     6061 aaaatggagc gcatggtttc atgccagatg ggagccgcgc ccgtaagacc cgtgacattg
     6121 tgcatcgctt cctgattgct gtttccgacc cacacaccaa ccagcaaatc gggagtgtat
     6181 ccgatggtcc aattatcgtg gaagttggtg gtcgtgcctg tttttaccgc ggcagtgcgg
     6241 tcaatcttca agacactgtt gatgccaaag cccagcgcac gagattggtc gtcgctgagg
     6301 atatcggaaa tcaaccatgc cactcgtgca tcaacgaccc gatgtgcggc ggggcgttca
     6361 ggctcgtaca gaagatttcc atctgcatcg cgaatttcaa gaacggaaaa caaacctgtg
     6421 acattgccct catttgccag cgccgcatac gcgcccgaaa gttcaagcaa attcatagac
     6481 ccgccgccga gcgccagaga aaggtcgtaa ttgcgcgggt cactcagtga acgaatcccc
     6541 aactgattgg caagcgagat cgcattttcg atgcctacat tttgtaaagt cagcactgcg
     6601 ggtacgttca aagacgaagc caatgcctga cgcactggca caaaaccatg ctccctgcca
     6661 tcgtaattca ctggcttgta gggctggccg tcctttgttg taaaagttgt ggcaacatcc
     6721 aaaatgggag ttgcagcagt aatcgcatcc atgcgcgttg ggtcaagtgc tgccgcataa
     6781 ataatgggtt tgaatgcaga gccagattgc ctgcgggtga tggccatatt gagcgcgcca
     6841 ttgatggaca catcaaaata atcggcgctg ccaaccagtg tgaggatttc acctgtatgc
     6901 ggatcaagga caaccagcgc ggcattctta acgttgaaat tggtctggcc ttcgagaggc
     6961 ttgaacagct cgatccgcct ttttacgatc tcctcggcaa ggttctgcat atccagatcc
     7021 aacgtggtgc ggataataag actctgttcc acactcaact ggccagacaa ataaagttcg
     7081 tccaactggt ctttgatcat ccagataaaa tgcggagctt cgatgggata aggttcggca
     7141 ttgtagctca acggagaatt ttcagcatca atccgctgcg tggctgtgat aaatccctcc
     7201 ttctccatca gacccagcac cactttttgg cgatccagag ccaggtctgg attggtgaac
     7261 gggttataaa taccgggggt ttgcggcaac cccgccagca gcgcacactc tggcaacaac
     7321 aagtcaacgg cgggtttccc gaaataagtc tgtgcagcgg cttcgatgcc ataggccatg
     7381 ccgccataat aaatttgatt cagatacagg gcgagaatct catctttgga ataagtttgg
     7441 gtcaattgcc aggccaggac ggtctcgcgc aactttcgac gcactgtgcg ttctgtgacc
     7501 tcgtccttta agagcagggt acgggcaatt tgctgggtga tggtactccc acccgcaagc
     7561 gtttccccac cgctgagatt gatccacagc gcgcggacaa tccccgtcag atccaccccg
     7621 gggttgctgt agaaattccc atcttccacg gcaatggtcg catctttcat acattgcggg
     7681 atgttctcaa ccgcgagaac agtgttccgc ccgcctactt cgggaataag ctcataaagc
     7741 aagcgtccat tcctatctgt aatgcggaca ctgggctggt tcaggctttc agggatggca
     7801 tgcaccgaag gcaggtcgta aaaaacggaa atgaaatacc cccccacaag aaaaatcacg
     7861 accagaagaa atcgcatcca cttggacttt ggcagggtac gcataaaatg acgatcgcca
     7921 acttttggga aaaatctaca tcacacgata gagaaaactg agcccgcaat ccatgcgggc
     7981 ccagggacaa cccatcggct acaaattcag cgtgaaatct tttcgagaaa gaaaagcctc
     8041 tacgtaacgg gcgtttatcg ggtctgatcc gccgttgctg ttcgtattat cgtcgtcatt
     8101 gctgttggca tcatcgttgc tattgtcatc attgttgttg tcgtcattgg tgttgtcatt
     8161 gctgttatcg ttgtcattgc tgttgtcatt aacgttgtcg ttcgtgttgt cattgctgtt
     8221 atcgttggtg ttatcgttaa cgccatcatt tacattgtca ttatcgttgc tgccgccatt
     8281 atcgttctca ttggtgtttt cgttggcgtt ttcattttca ttgctgtttt cgttggcatt
     8341 gtcattacca ttgtcgccgc cgtcgttgtc attgccattt tcgttatcag tgccgccatt
     8401 atcgttgccg tcgtcatcgc cgccaccgtc attgttatta tcgccattgt cgttaccatt
     8461 atcgttgcca ttgccgtttt cattgtcatt gatattttcg ttggcgtttt cgttctcatt
     8521 aacgccaacg ttatcattgc cgttgttctc attttcttcc gaaccgctgt tatcgttgcc
     8581 gttcaggttg atattttcat tctgattgtc ttccaccttg accggctcac ccgtgggaac
     8641 gactgtccct ggaggcagaa gtatgatgga cgccgcatcc acaaaacctg ccgcgttggt
     8701 gcgcccggtc actgtgacgg ctgcaccgtt tacaagcccg tccacgggaa gattggtcgt
     8761 gtccaggatc gccacctgaa tacccgaaac ataggtcagg ccattgacat ccataaagat
     8821 gcctgaaaac tgaatggttt catgcctgcc ttcggcaaga agttcactga tctcatgcag
     8881 gcgttcattt ccaaaggcat gttcaagcac ttcccgggcc gcatgatcaa aggtaaagaa
     8941 gagacgcaca ttctcccagg tgagtttgac ggggtacagg ttttccccgg gcagggccga
     9001 agaagaggca cccaccaaac cagtgccgct agcaaggaac agggcggtca tcgtaaatga
     9061 gatcgccatg cgctgaaaga agggaatgac tcgtttgcgt ggtgcaagct ttgcttcgcg
     9121 catttcagca gcatgctgca acaattttgc acgcccgcga cgaaccacct cgggcgaggg
     9181 ttcttgagcc atggtccgtg cttcgacgga tgcttttaag atcgggcgca gttcaccagc
     9241 aaagctggga tagcgcgcga gaagggcatc catatctgca ccattttcaa gttcctgaag
     9301 gcaaatttcc aaaatgtcgt atatgttatt cataattcac ctcaccaacc tcgcgctgaa
     9361 gagcggccag cgcacggaac tggagagcct tgaccgcgtt gacgttttta ttcataaagg
     9421 ccgcagtctc gtctaaagag tatccctgtc caaaacgcag ggcaagcaca ttctgctgct
     9481 caggagttaa ttgcgcgtac gcgttgttca ctgcctgatt ctgttcccgt tgatccactt
     9541 cagaaggcac atttgcgcca gaatctggaa gggattcgtg gagttcgttt tccgggcggc
     9601 gatatttccg ccgcaggtgg tcggtgacca aatgggaggc tgtaccgatc aaccagcctt
     9661 tcaaacttgt cttcgggggc tgtccatttt gaacggcctc cagaagacgc acaaatacat
     9721 cacttgcgat atcttcggcc gcatttgaat ctccgatgcg gtagcggacg tagcgataga
     9781 cctcggaaaa atactggtcg tacaccgcgc cgatcgtctg cgcatccagt ttttgcagtc
     9841 catccaattc tttttgcagg tcattgggag gcataatatc tctctcccgg gagccatgaa
     9901 atcaaatccg acatggcaga tccatcagat agggcggcat cgtattctgt aacgctcccg
     9961 cagcacgggg ctatcaggaa aaggcgtggg gatgtccttt tcatttcaaa gctttctcca
    10021 tcaactgcaa cttgcttgat catcatcatc cttcacaaac tcaaggcgtt atcacctcaa
    10081 aaatcaccgc ctgaccatca cgatacactt ccttcagccc tgggtcttcg gccgccactc
    10141 gcagaaaatt ttcgtggttc aagtcactgt gaacgtagcg ggcttcaaat tgtgtggcaa
    10201 tgatctccga tggattgggg gtcttgccgc gggtaatgct tacccaggta tcgtacattt
    10261 ctgcatcgta caattgcaga taggtcggat cgaggcctat caggtaggtg ttatgggtat
    10321 tataaaagaa caagcgcgga aaatcatccc aatctgtctg gaaaacgcgc tccccttcag
    10381 cagtgtgttc caccagccag gcagatgcat cggcgtacaa ttcataggat ttggaacgct
    10441 gcatctgctt ttgtacggca gggatggatt tcaccatacc cactacgacc gccagcataa
    10501 gcaaaaggcc gggcatgttg gccatgagct cgtcccactt cgaacgggag aatctggatt
    10561 ggaaaccaga agcaggctca gggtccacaa aaagaggcgc ccaggcaaat gcggcaaaga
    10621 tcaaggcgaa gggcggaaaa tattccacga agcggcgtgc ctggaaaagc atcaatccaa
    10681 agagcaatgc gatcaaaaag ccggtcgcgg ttcggacatc cattttgcgg ccagttaacc
    10741 caagggcgag tgcgccgctg acaaaggcca ccagtgcagg cagggagttt tcaagcagtt
    10801 gcgccgtttc atagggatac cattcgttgc caacactgac ggaagtggct tcaaccaatt
    10861 tgggcagcag gtggcggtag gtaaaaacca gattggcagg aaaatatgga ttgatgatca
    10921 cacccagaac cactccaatc cctgaccaca acagcaggcg gtattccaag cggcgctcga
    10981 tcatggcaac ggcaagcaag tgcaacacgg tcaatgacat catcaggggg aaagcgtcgt
    11041 atgcccaaac gtaaatgaat gagagcactg caaggtgttt atacttgcct tccaaaatcc
    11101 aggccaatgc aagcgccaga attccaagag agagggattg ggcgcgggtg atgctcatgc
    11161 ggtataaaaa tgcgtcggaa ataccaagca accccagggc ccacagccag gccagcggaa
    11221 ttcgctgacg atgaaacaag taccagaccg ccaagaaagc caggcttgaa aaagtgacag
    11281 ccgcccattt ggcacccaca tgcaaatcgc caaaagtgaa ggggatcaag gcaacatgga
    11341 acagaaagtg gtgatcgtaa aactcttttt cgctgagaat gctcaagggc agccagacaa
    11401 aatcgggttt cagtccttca gtgcgcatga gccaggccag tttgatgtga taaaagccat
    11461 cattatcggg catatcggag gttgcaaatt gaattgcagc catgccgatg aaaaaaagaa
    11521 cgaataatag caggggaaga aatttggaaa tggtttgctt ttgcattgaa atacctggcc
    11581 cgttggaata agagcggctc gcctgaagga ttttgctcca ggcgagcctt tcgatttgaa
    11641 tttcagtcgt tgagacgatt agccgccgtt ggtgttgccg tcgttggagt tgtcgtcgtt
    11701 gctgttgtcg ccatcgtcgt tggagttgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn ccatcgtcgt tggagttgtc atcgttgccg ttgtcatcgg aaccgtcgtc
    11881 gttggagttg tcatcgttgc tgttatcgtc ggtgccgtca ttgctgttgt catcggtacc
    11941 gtcgttggaa ttgtcgtcgg tgccgtcgtt gctgttgtca tcaccatcat catcagtatc
    12001 gtcattcgaa ttgctgtcat tcgagttgtc gtcggtaccg ctgttggtgt tgtcacctgt
    12061 gccggtgttg gtgttatcgc cagaaccagt attggtgttg tcgccggtgc cagtattggt
    12121 attgtcgcca gtgctggtgt tatcgttgct gccagtgttg gtattgtcat cagaagatga
    12181 cggggcttca aggcgggtca ccacaatgga accatcttca gccacggagg cttcgatctt
    12241 gacggtgtcg ccgactgcgt aggtaccctg caccaatgcg gggtcaactg tgatggtctg
    12301 gccgttgaca acccaggtat ttccatcaat ggattcgatc acacctgtga actcaacacc
    12361 agatgcctca accttggcgg cgccggtatc ggttgtggtg gcgacaggag cacttgcaca
    12421 agcattcatt accagggcaa ggaggaccag aacgggcctg agatagcgga agaattgttt
    12481 catgattttc acctcttttt gtttgatttg gttacatacg agtagtcgtc aaagaggcga
    12541 aaaggttacg gctttctccg tgcaattttc aaaactcgtt tttcttttgt gaaagaatac
    12601 accagtcaat tcttctctat tgtgccgaac ggaacaccct gaagaactca gccttgaatt
    12661 gggcggcaat atcggcatta tagatgacca aaagtgtttc atcgttattg gtctcggcgc
    12721 tgttcgtaaa attatacgag ccaaacacta cgatgctgtc atcaatgatc attaccttgt
    12781 ggtgcatcaa gccagggatg ttatcttcat acacctcaag gcctgcctgt ttgaatgggt
    12841 catactcagt gcccaggttg gatttgatct ggtcggcatc catcacccct gccacggtca
    12901 acccttcggc agaggcagag cgtatggcat tgccgatcgg gtcggcagta aaggaatatg
    12961 ccataaagta aatgctggcc tgtgccacag aatccaccag gtcgaacaag gctgcctgtg
    13021 catggtcttc gggtgcaaaa tacacatcaa tattcgtacc atcaatcgtt acccgcggat
    13081 tcggggtggg gttacccaga tcggggccaa aaatatcatc cacgaacatc tcttcgaatt
    13141 cagcagtgaa atcttcggca agtttcgtag agcggatctt catcaggttg ttattgtctt
    13201 tgtaggcgcc agagtcggta aaattcatcg agcccagcca aacctccaaa ttatcgatca
    13261 ccacaaattt attgtgcatc aagccttcgc ggcgatcacc caaaatggga atcccagcat
    13321 ctttcaattt ctgcgggtca gaacggtcaa ggttatcgct ttccatcacc atgcgtacgc
    13381 gtacgccgcg gtcgtgcgcg cgcagcaatg catcacgaac actatttaga ctcaaactgt
    13441 aaattgccac atccacgctc aaacgggcat tgtcaatggc gttcacaagc ggaccatcag
    13501 ggccgcctgt ttcctgtgcg gcaagcgggc tgctcgggtc ggtgaaatac aactcgaacc
    13561 agtcgccgcg cacgccatac cctgcaccaa gctcaaccac ggtcaacggg ttggtgaaat
    13621 ctgattcgat caccgtaccc actacaggcg gagtcggatt cgcttctgag gtggtggtac
    13681 atgctgtcaa taagatcaag ataaaaagca ggctcacggt ctttttcatg gaattctccc
    13741 aaaacgatta taatcccgcc accctatgtc agaaacaaac ggaaatgcca atcgtcagat
    13801 cgcgcgcgcg gctggcacgg tgatggctgc cattttactt gggcagcttg cagggcttgc
    13861 ccgcggcgtc atcgtggctc gcgcatttgg agcctcgccc gagcttgatg ctttttttgc
    13921 agccaatcgt gtcagtgaaa cattgttcct gcttgtagcg ggcggtgcgc tcggatcagc
    13981 cttcattccg atgtttacgg gactgctcgc caaaaacgaa aatgactcag cctggaggct
    14041 ggcctctgcc attggaaatg cggtcacact gaccctcagc ctgcttgctg ttttagtggc
    14101 agtcttcgcc ccgcaagtgg tccgctacgc actggcccct ggcctttcca gtgacccgca
    14161 acttttcgct ctcaccattt cgctgctgcg catccaactg atatctgccg tgctcttcgg
    14221 catcggcgga ttgatcgtcg gcattttgaa cgcgcatcag gtctttttga tccctgcact
    14281 cacgcctgct ttttatcaac tcggaattat ttttggcgca ctggtcctcg ctccaacgct
    14341 gggaatctac gggcttgcat ggggcgtggt catcggctct gtgctgtatg ttgcacttca
    14401 aaccccaact ctggcaaaac ttgccgcccg ccaatcacaa ctcgccacct ttcacttctc
    14461 tcttggcctg catgatgcaa acgtccgtca ggttttgatg ctcatggggc cgcgcttgat
    14521 cggcgtggct gtggtgcaat tgaatttttg ggtaaataca tggctggcct ctcaaatggc
    14581 cagtggcagt gtcacagggt tgtattatgg gttttcgctc atgcttatgg cacaggcagc
    14641 cattgcgcaa tcagtggcca ttgcagccat gccaaccttt tcagcgcaac atgccctcgg
    14701 ccaaacggac gagatgcgtg cgtcattggc ttcctctctg cgcggcgtca ttctgctagc
    14761 cctgcccgcc agtatcgggt tgatgctgtt gcggcagccg ctcatttcaa tgctttatca
    14821 acgcggaaag ttcgatgcca ccgatgtgca gatcgtcgcc tgggctttgc tctggtacgc
    14881 ggcggggatc gttggccatt ccatcatgga gatcttgacc cgcgcctttt acgcccaaca
    14941 tgacacccgt acccccgtta tcatcggcac aatcgcaatg gggttgaatg tagccttcag
    15001 tatcctgttc gcaaaactgt tcacggccgt cggttggatg ccccacggcg ggctggccct
    15061 ggcgaattca tttgcaacgg ctcttgaggc aactgctctt tttatcgtga tgcgcaaacg
    15121 actacaaggc attgaaggtc ggcacatttt gggtggagcg ctcccctcaa cagcggcggc
    15181 agtagtgatg gccttctcca tcattggctg gctctcggca ggaaaaaact taaacgcgtg
    15241 gatactcgcc ctcatcggcg tgacgatcgg aggcggggtt tacttcgcca tgttatggat
    15301 actgcgtgtg cctgaattgc agtacatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnaaa aacgtccttc ataaaagcag aaggacgctc gataaaagta aacaaacaag
    15481 cggggatttg atttatctac ggccagatga tccactgcat gatggctaca aagaagccaa
    15541 gtaaaattcc gccaaccact tccagcggag tatggccgat gacttccttc aattcatttt
    15601 catcccacat gtgccccttg agcagttctt cgaacaacac gttaatgcgt tcggcgtgca
    15661 tccccgcctg cctgcggacc cccgccgcat catgagcgat gatcatcgtc acagccacgg
    15721 ccagcccaaa aagcgggtta tcaaacccat gatacaaacc aaccgcaatg gtccctgaaa
    15781 ccatcaaggc cgagtgagag gatggcattc cgcctgccgc aaaaaacatg gcccacatcc
    15841 aacgacgaga gcgaaaatac tcggtgggaa tcttcagcgc ctgcgcgatc aaccaacccg
    15901 ccagcacagc gatcaacact ttgttatcca aaagcgccgc caggttcatt cggattcttc
    15961 ctcgaactcg atcaaggatt cgccagccag tttctgcacc ttcaactggg cagattcaag
    16021 caatgcgccg cactgcctga ccaaagcctg cccccgttca aaaagcttca tcgaatcttc
    16081 aagcggattc tgctcatctt cgagcgttga aacgatctgt tcaagctcgg ccagcgcttc
    16141 ttcatatgac aattcttcga tggattttga cgattttgat ttagccatga aaaccactcc
    16201 aaaataatgt ttgatcctac gacttgcgat ttacgtcgaa ttctccgtcg ctgacacgca
    16261 ctttcatttc actatcggca tcagccacct tgccgaccac tctgccatcc actttccgcg
    16321 tgatgatagc atatccacgc gcgaggattc ccatggggtt caacgattgc aagcgttttg
    16381 acattccatc tacgagagag gcctgcaatt ggatgcggtg atcgatggca gaccatgcac
    16441 ggcgggagag ttcgtcgagg cgttgatatt cagattggat acggcgctca ggagaggtgt
    16501 atttcaaacg acctgccaaa acggagagac ctgccttttg ttcggcaagc aggttcgagg
    16561 ttaagtcggt gaggcgggtg gaaatcgtgg aaagttgaaa atggagatcg tccagcgttg
    16621 tttgggtggc aagttctgca gcagcggtcg gagtcggggc gcggaggtca gcggcgaaat
    16681 cacacaaagt gaaatcagtt tcatgaccta ccccacaaat cacaggtgct tttgaagccg
    16741 cgacggcgcg gacaacctgt tcttcattga aagcccacag atcttcgatg gaaccgccac
    16801 cgcgtgcaag caaaatgaca tcaggcttct gtcgattgag agaattcagc gcattgacca
    16861 gggcaggcgg cgcctccacg ccttgcacag gcgagggagc caaaatcaca cgtgccagtg
    16921 gctggcgtcg gcgcagtgtg ttgagcatat cgcgcaaagc ggcgcctgtt gctgaggtaa
    16981 cgatgcctat ggtttgcggc ctgtcaggga tcaagcgttt gcggtcagcg gcaaacaacc
    17041 cttcagattc gagcatggct ttgagccgca tgtattcctg aaacaacctg ccttcaccct
    17101 tgggctgcac aaggttgaca atgagctgat actgtccctg cggttcatac accgtgatcc
    17161 tgccgtgaac ttccacggcc atgccatctt gcaagttgac ccgtaagcgt cctgcatcca
    17221 gtttccagat cacacaacga agtgaggcat tcttatcttt cagcgtgaaa tagatatgtc
    17281 ccgaggcagg tttcgaaagg tttgaaattt caccatccac ccaaacatcc tgcagggtgg
    17341 gatcatcctc caattgtttc cgaatatgga aggtgagctg ggaaaccgtg agatgcacag
    17401 aagaaaacaa agtcggttgc atgagtggcg aggataactt aaaaaggatg aatatggaag
    17461 gatggaaaga aaaacatcaa aaagcgggtt tcttctttca tctgtcatcc tttataattc
    17521 aacttaatac tcccatgaat catctctggt caccctggcg catgtcatac attgaaaacc
    17581 acacaaaaga gaacggctgt gtcttttgca ttgcacaagc caaagcggac agcgaagaaa
    17641 acctcatcgc cttccgcgga aaaaacgcgt acgtgatcct caaccgcttc ccctacacca
    17701 gcggacattt gatggtcatt cccttttcac acacatccaa cctggaagaa cttgatgcgg
    17761 aaacccgcgc cgaaatgatg gaactggcct cacgctgtac cacggttcta cgcaacgaat
    17821 atcggaccca atcgttcaat atgggcatca atatcggtga agcggcggga gcgggcgtac
    17881 tgggacatgt ccacattcat attgtgccgc gctgggtggg cgacacaaac tttatgtcat
    17941 ctgtgggcga aacacgggta ctgcccgaaa cgcttgaggc tacctacaaa cgcgtgcggg
    18001 atgccttcca gacaaacccc taaaatgcac aaatactccc ttgctttgaa atcccttttt
    18061 gagaaaaatg ccaaccctgc acaggcaggg cccatgcaaa aatacatgcg agaccaattc
    18121 gaatatctcg gaatcaaaac gccgcaaagt gcggcgctga tgaagggatt cgttgccgaa
    18181 cacggactcc ccccgctgga aaaattggat gtgatcgtgc gtgacctgtg gtcactaccc
    18241 cagcgagaat ttcaatatgc cgctacgggg ttgataggca aactcgaaaa gaaggttgaa
    18301 cctgaattca ttacgaccat tgaatatctg ctggtcacaa aatcgtggtg ggatacagtg
    18361 gatacacttg caagtcatgc agtggggtct cagttcaaac gctttccaga aatccgcaaa
    18421 acatacctga aaaagtggcg aaaatcggaa aacttttggc tgcggcgtac gaccctgctt
    18481 ttccaactag gttataaaaa agaaacagat tttgacctgc tgtgcgagat cgtcagggaa
    18541 aacctcggtt cggacgaatt cttcatcaac aaggccattg gctgggcatt acgccagtac
    18601 gcacacacca acccgtctcc tgtaaaaaag tttgtcgctg ctacaaagga actgcatcca
    18661 ttaagtcgac gcgaggcatt gaagaatatc gaggagtaaa agtatgaaga ttgacagcac
    18721 accttgcctg tcgtaaaatc tagacagact agacataaag gagagattat gaattcggta
    18781 tggcaaattc aagatgccaa gaacaaactc agtgaagtaa ttgctcgagc cctacaacag
    18841 ggtccacaat taataaccaa acatggcgaa aaaaccgttg tagttatttc ttatattgaa
    18901 tatgagaaat tgcgcaaatc tcaagggaaa atctccgagt ttttcagcgc ctctcccttg
    18961 gctggaatca accttgcccg ggataaatcc attccacgaa aaggacttga gctgtgaaat
    19021 atctgcttga cacttgcgtc atttcggaat taattgccaa aacacccaat ccaaatgttg
    19081 tagaattcgt tgacgcaatt gagacagaca atatattctt gagtgcaatc acaattggag
    19141 aaatagccaa gggtattgaa aagcttcccg aatcaaaacg aaaacgagaa ttacaaacat
    19201 ggctcaagga agatttgctt gttcgttttg atgataaagt cttacctctt gatacagaag
    19261 ttttaattca atggggaatc ctgtctgcac gacttgaagg cactggcata acacttccct
    19321 ccatggactc cttgattgcc gccacagcct taacccacaa cttaaccctt atcacacgaa
    19381 atgagggaga ctttcaaaat acggggattg agattattaa cccgtggtga gtgagtctac
    19441 gtgacctttt agctcaacac cctatccttc ttctctattt cgcataaaat ccgccacttc
    19501 ggcgaaacgg gttctgagat gttccttcac agcagggagg atattcatct cttcaacggc
    19561 ttgattcatg caccacagcc actggtcacg ctcaatgccg ccgatggaaa atggcatatg
    19621 gcgttgacgt aaacggggat gaccatattt ctcaatgtaa agcggcggcc cgcccgacca
    19681 gcctgtaaga aagagaaaaa gtttttcgcg cgattgtttc aagctcgccg cgtgcaaggc
    19741 acggatatcc ttcgcctcgg gcgaagaatc catgatgtcg taaaatcgtt caaccaaagc
    19801 gcggacgccc acttcgccgc cgagcaggtc atagagggag ttaagttcat tcatgggtga
    19861 aattgtaata taagtttcat aaacaagttg ggaatgacag ttcatcattg ttatgagaaa
    19921 atagaaaata atgaccgcgt accaacagga gaacccatga gcaaagaaaa tgacgcagtg
    19981 attctaagtg cagcacgaac cgccgttggc aagtttcagg gtgggttaag cggcttttcc
    20041 gcaacaaaac tcggtgccat tgccgtacag gcagcagtgg agcgtgcagg tattgacccg
    20101 caaaacatcg aggaagtcct gatgggcaat gtcgtccaag ccggcaatgg tcaggcgcct
    20161 gcgcgtcagg cggctatttt tgcgggggta ccagccactg tcagcgccac ggctattaat
    20221 aaagtttgtg gatcaggcct caaggcggcg atgatgtcgg cgcaagccat ccgcgctgga
    20281 gatggagacc tgttcgtggc aggcggaatg gagtcgatga gtcgtgcgcc gtatcttgtc
    20341 agcggacgtt cgggtgaact caaatttggc aatcaggccc tcaccgatgc cctgctcaac
    20401 gacggccttt gggatccttt tgaaaattgg ggaatgggga tggccgccga attcattgcg
    20461 gatgaatacg aagtgactcg cgccgcgatg gatgaatttg cgcttcgctc tcacctgaaa
    20521 gctgttgagg ctcaagaaaa gggcatgttc agcgccgaga tcgttcctgt gcaaatccct
    20581 ggccgaaaag gggaagtgac gatcttcgac aaggacgaag gtcctcgcaa ggacacttca
    20641 ctggaaacac ttgcaaaact caaacctgcc ttcaaagccg atggaaaagt gaccgcaggc
    20701 aacgcctctt ccatgaatga tggcgcggcg gcagttgtga tctcgtcacg tgcttatgca
    20761 gaaaaaaaca acctcaaacc tctcgcgcga attgctggct acgcgcaggc ggcattggaa
    20821 cccaaatatt tgtttgccgc ccctgctcat gccattccaa agttgctcaa gaaaattgac
    20881 tggaaactca gcgacgttga cctcatcgaa gtgaatgaag cctttgcggc acaggtgctt
    20941 gcagatggtt acgctctcgc cgatcagggc tgggactggg ataaggtcaa cgtgaatggc
    21001 ggcgcaattg ccatcggtca cccactcggc gccagcggtg cccgtgtgtt gacgactctc
    21061 atctatgccc tgaaaaatcg cggcctgaaa cgcggagtcg catcgctctg cctcggcggc
    21121 ggcgaagcgg tagccatggc agtgga
//
LOCUS       DAOQLC010000075        21061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_872_length_21061_cov_24.241740, whole genome shotgun sequence.
ACCESSION   DAOQLC010000075 DAOQLC010000000
VERSION     DAOQLC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 21061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_872_length_21061_cov_24.241740"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /gene="miaB"
                     /locus_tag="PL000_13490"
     CDS             <1..1362
                     /gene="miaB"
                     /locus_tag="PL000_13490"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNC89963.1"
                     /translation="MKYHIWTEGCQMNVADSQRVGSSLEHLGYTFTETIEEADVIVLN
                     TCVVRQSAEDKALGRLSSLAPLKKKNPNLVINLMGCLVGVRGTEKLKARLPYVDVFSP
                     PSDPGPLISHLTQGEIRSMEDAETTRRFLMMDDELILPVAEQGKLVTAHVPIVYGCSH
                     ACTFCIIPYKRGVERSRPVGDIVSEVRSLAKQGVKEITLLGQIVDRYGKDIPDGPNLA
                     ALLRIIHDEPGAEGIERIRFLTSHPNYFDDDLIQAIAELPRVMPHIELPIQAGDDEVL
                     ANMKRGYTQQNFRDLVEKIRARIPNCSIATDIIVGFPGETDEQFMETYRVLSDLKLDV
                     AHLARYSVREGTVATRRMEDNVSDEEKRRRFHLLEELQEQVVGEINKKYLGETVDVLF
                     EEKVKGRWRGRTPTNKLVFVESEEDLRGKLRPVTVTWTGPWSMQAQLAGVRSQNKPEL
                     ISL"
     gene            1424..2341
                     /locus_tag="PL000_13495"
     CDS             1424..2341
                     /locus_tag="PL000_13495"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNC89964.1"
                     /translation="MSFNELITTFANNLLPILLVSGAGFILGKFLTVDSRSLGRVVFY
                     VFSPLLVLDLMLKSQLNLQQAMTTVGYTMSVIAIMGLLAFLFGKLFKLEKPYLLAVIL
                     TVAFGNTGNYGLPLVKFAFGTDALAVASLFYVTTTILFNTVGVVIASIGHMDLKSAVL
                     GLFKLPSIYGVTLALIIKGLGIQLPLPISRTIEIAANGAIPVMLILLGLELTRIEWSH
                     SFRALGLGVAVKLLLGPLVGLALAGLFKLQGHVRQGAVLEAAMPAAVATTVVATEYKL
                     EPSLVTAIVFLGTALSPLTLTPLIVFLTR"
     gene            2344..3570
                     /locus_tag="PL000_13500"
     CDS             2344..3570
                     /locus_tag="PL000_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HNC89965.1"
                     /translation="MPHLKKLIARLMAILLTAAILPFNLNQAGAQGGIDVTAATAVVD
                     FGRTITFQATITAPIGIQQVSLLFRGMNEDVTRVEPVQLGPDGFVSFTYDASRNFFPP
                     FSSVVFWFQATLADNQTYTSQPITFAYNDNRFPWREARRDNLVVHWYAGDDAFGAAAL
                     DAANRGILSINEFIPISSSQPIDIYIYSNALDLQNTLLLGGQEWVGGHANPELGVVLV
                     AISPGVNQSIELETMIPHELAHVMLYRSLGGTYNIQPLWLLEGIATMAEYPNPDYKHA
                     LDIASGNNTLLSFADLCVSFPADAGNAYLAYAQSQSFVTYIRETYGTSGLARLTKSYS
                     EGFNCELGATNALGTPLSLLDVRWRESVLGQNVAGVAFRNLSPYLLLMALVLVVPMWG
                     AIDMLRQRRKRAKQSD"
     gene            3551..3853
                     /locus_tag="PL000_13505"
     CDS             3551..3853
                     /locus_tag="PL000_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNC89966.1"
                     /translation="MQNNPTDELKRVHMWVKGRVQGVGFRAHVEYHALQIGVLGWVRN
                     LGHDTVETVAEGTKEQIAQFIEMVKTGPRHSRVDEARVEYEPTTGHLSGFTVKMSM"
     gene            complement(3856..4563)
                     /gene="radC"
                     /locus_tag="PL000_13510"
     CDS             complement(3856..4563)
                     /gene="radC"
                     /locus_tag="PL000_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNC89967.1"
                     /translation="MTENNSKPVYRIADLHESDRPRERLVALGPQALTSAELLAILLR
                     VGVVGENAVEVGQRLLKKFGGLSGLHRAPIKELMDQHAIGEAKAAQIKAAIELGRRLT
                     LEAPEERPSINSPADAAALLQFEMSALEQEHLRVLLLDRRNRVLETVEVYKGSVNSSQ
                     VRVGELFKDAIRANASAIIVAHNHPSGDPTPSPDDVAVTRAMVQAGKLLDVDVLDHLV
                     IGLGRWVSLKERGLGFS"
     gene            complement(4649..4804)
                     /locus_tag="PL000_13515"
     CDS             complement(4649..4804)
                     /locus_tag="PL000_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC89968.1"
                     /translation="MLFAPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKV
                     NSSLSNV"
     gene            5120..6394
                     /gene="hutI"
                     /locus_tag="PL000_13520"
     CDS             5120..6394
                     /gene="hutI"
                     /locus_tag="PL000_13520"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNC89969.1"
                     /translation="MLIHSASQLLTLAGGPQRGHALGTLGIIENGAVIVRDEKIVAVG
                     TTEELKNAYPDEPTLDAGQSVLMPGFVDPHTHVIWAGDRANEFEMKMGGMAYLDILAA
                     GGGIISTVKATRTASLETLIAQTRPRLLKMFSHGTTTAEAKTGYGLQTSTELRLLKAL
                     IALDDESPLDLAITFLGAHAIAPEYKDNPQGYTDEVVNTMLPMLKEWWETHAPRLPLP
                     FVDVFCETKAFDLEQSRQILTRAGSLGFPLKIHADEFDNLGGAALAAELGAASADHLV
                     RTSDADIAALGKSDTVAVALPCTPFGLAEKDYTPARKLIEADAMLALATDCNPGTSWN
                     ESMQFAIALACRYMKLTPAQAIAAATINSAHAIRRSDSIGSIEVGKQADMLILTVPDY
                     RQVGYRYGTNLVKQVIKRGRVYSVDVGYYRTA"
     gene            6407..8023
                     /locus_tag="PL000_13525"
     CDS             6407..8023
                     /locus_tag="PL000_13525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC89970.1"
                     /translation="MDIRWIIFGVILVINSIFAITTIVLITRKFDVAGRDALIMMLCS
                     LAIWSFAYAMITFSSDLETKKFWLRVENVGILSQPVFWIVFILGYSRQGRALNWGYIA
                     LLSIVPAVSMIMIASDRWFHLYYSSVKLLDGNYGPLVIERGPWYGVAVFQAYALDLIA
                     TILLIWRFIQFRNIFRTQLVFLIVAALIPLFTNIGYQVVSIFLPSLFLPVDLTPISFT
                     LSISLISSGIFRTRLLDLVPIARDVVMEHIPEMVFVVDAHDRVLDVNTVAEKWLGMTR
                     DQIIGKDPIEIFGKWPDLYNRFLTTEKTREEVKIYGDPPLTLEVVITPLYNNEKINGR
                     VVLAYDITKRKQLELDLKAKIIEIDSLRVQLQDQAIRDSLTGVFNRRFLAEALEREIA
                     KANREDMPISIVMMDVDHFKSFNDTYGHKCGDAVLRDLAGFLTKNSRHGDIVCRYGGE
                     EFVILMPEASFQNAFERAQSWQQAYASKTIEYEDKALKVTFSAGVASYPQHGPNGETI
                     LHAADHALYESKSNGRNRVTAFQKRLTKEN"
     gene            8026..8931
                     /gene="rocF"
                     /locus_tag="PL000_13530"
     CDS             8026..8931
                     /gene="rocF"
                     /locus_tag="PL000_13530"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HNC89971.1"
                     /translation="MQIDIIGVPIDLGADRRGVDMGPSAIRYAHLQKKLEDLGYDVHD
                     EGNVEVPIAEMCKITNPKLKYIDCIIPMSRRVAGAVATSIQSKNFPLVLGGDHSLSIG
                     SVRGAARNKKIGVIWIDAHADFNTAETTPSGNIHGMSLAVLAGLGDKSLVQLWDENIP
                     VIDPSKIAIIGARDLDSGEKVNLSNAGAMVMGMEQIDRYGMVSVVEKAIERVSRDVDG
                     IYLSLDLDSLDPEHAPGVGTPVSGGLTQRETHLACELIAETGKLIGMDVVEVNAILDV
                     QNRTASLAVDLALSALGRRIWNGQS"
     gene            8928..9698
                     /locus_tag="PL000_13535"
     CDS             8928..9698
                     /locus_tag="PL000_13535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNC89972.1"
                     /translation="MKPTLKEVEQLARGAGAILRERYNQEHEISYKGVIDLVTEADHA
                     SEAFLIGEIQSKFPGSHILAEESGETKGSNEGVWYIDPLDGTVNFAHHIPVFCVSIAY
                     AFEGSMLLAAVYDPLRDEMFTAERGKGAFLNGKPIHAAKTTELQRSLLVTGFPYDTWN
                     TEKDNFKNFEKLAKMTQGVRRLGSAALDCSYVGAGRFDGFWELTLRPWDIAAAGLIAE
                     EAGAKVTATDGKPDYISAPQSILATAPGIYQQMLEQLN"
     assembly_gap    9708..9807
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9808..10821)
                     /locus_tag="PL000_13540"
     CDS             complement(9808..10821)
                     /locus_tag="PL000_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89973.1"
                     /translation="MKKLTACLFIFSSLWLNACGSPGHNSNLNASILPDDCRVQAGGQ
                     LALTLDGQIPANTQIQWSASTGEVVWTGQGLTATFLAPNLPGEAVISVLFVSGTPAPY
                     TASRACAVTEQDSTASAPTVNDPAAITIAISEVMGNQCGGLETRKFNQYVELYNFGDL
                     PVDVGSWMLFDEGEKGTPDRLVAWDSRSSLQLDPTLVTNSTVIPPRSFAVVLSPLYPE
                     GNIENRMPYKFPPGVVILTAESSQTLGDDYFGIISDQDGYDTVTLYIGSDTVMDLVVD
                     TYGTPTINGPYPVNIDDNHLDHTPLHLSECESAERIDPRQPDSEANWLALPGGSPGDG
                     PYQ"
     gene            complement(10825..11478)
                     /locus_tag="PL000_13545"
     CDS             complement(10825..11478)
                     /locus_tag="PL000_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89974.1"
                     /translation="MSRKPLEFISGLAVYIVLTLLIAFAVILRLSVVPVQFINWLGQS
                     HNPQNALAYFSMAFSATILVFLRFGIEIPRIKMVTSSEKLQPYLSGLPLWSLGLLWAG
                     SLIAFWNVFPSCQPPVTIQFEISGREGFFQPSDRVPISPGEVVTVTAHSVNTDTTLSC
                     SWEYAGGIFETIGAQRGCQVSAKFNQQTGSGFMTVSVSENFCSQTSIFSLQPVIGTP"
     gene            11580..>13050
                     /locus_tag="PL000_13550"
     CDS             11580..>13050
                     /locus_tag="PL000_13550"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC89975.1"
                     /translation="METKKLILVTGATGYVGGRLVPKLLEAGYHVRCLVRDPSRLQGR
                     SWLKRVEVISGDALSPEGLAAAMQGVSAAYYLIHGQQGGKIDAERDLQVARNFAQAAE
                     DANIERIIYLGELVDPTANLSPYLRARHETGYILRYGSVPVTEFRAGMIVGSGSALFE
                     MIRYLVEREPLLICPAWFFSQAQPIAIRDVLSYLVDALKTPDSNGRVIEIGGPSRLTY
                     ADMLLNYAKERSLKRWLIPTPFNAPRLSAYWVHMITPIHWRVVAPLIEGLRARLIVRD
                     DTARKLFPQIKPIDFQTAAHLALGRIQRDNVETSWADALVTSTGDIKPYEFTVEEGMF
                     IERRQILLDLKPESVFRAYSGIGGERGWLYMDWSWAIRGWMDKAIGGVGLRRGRRHPD
                     EIHTGESLDFWRVENVEKNRLMRLRAEMIVPGKAWLQFESTPQGTQEEKTLLTVTAYF
                     APHGVPGFLYWYAMWPFHKFIFDGLARQLASRARVLSHSY"
     assembly_gap    13051..13150
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13151..14451
                     /locus_tag="PL000_13555"
     CDS             <13151..14451
                     /locus_tag="PL000_13555"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HNC89976.1"
                     /translation="TSMKPLLILVTGATGYIASRLIPQLLDLGHTVRALARRPHQIGP
                     RNWSAHIDIFPGDVTNPASLAPALKDVHTAYYLIHNMSSGHGYTDIELQAARNFARAA
                     EEAGVKHIIYLGGLADPEQHIAPHMRSRIETGATLRAGKVPVTEFRAGVIAGSGSISF
                     EMIRFMTELFPIIPAPAWIQNKSQPIAAQNVIDYLLAALENWDGHGKVFEIGGPDITT
                     YQDLMLRYAHIRGYKRSFLVLPYVPVWFMAFGIGLMTPVPRPIAHALVGGLSSDSVVM
                     HDHARAVYSKIKLIDFDSAAKEALTHLHPHRIERVWDDGTGRSKTLKHEGFFIAHHEA
                     KTNAAPEQIFTAIKNMKWQEFVFDSIEENRILLLRSKHTSVGPMFLEWRLTNQNHIAL
                     TYYLAPRGPLGFLYWHLLYPLHTYQLRKLLKRVVRNSVVK"
     regulatory      14424..14620
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            14732..15706
                     /locus_tag="PL000_13560"
     CDS             14732..15706
                     /locus_tag="PL000_13560"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNC89977.1"
                     /translation="MFRKILVLALLLALFITGCGPARSATPTEVPAPVSTEIPATEAP
                     SSSAEIQLTDGLGRSVVLKEAAQRIISLAPSNTEILFAIGAGSQMVGRDSLSDYPAES
                     ANVTDIGSAYEALNTEMIISLKPDLIIAAEINTPEQVKELEDLGLTVYYLNNPHTLEE
                     LYDNLQIVAQLTGREAETTALIDSLKARVAAVDEKIAPLSSRISIFYEIDGTDPAKPY
                     TAGKDTFITLLIERAGGYNIAADIEGYPQLSLEQVVAADPAFILLGDAAYGVTPESIA
                     TRPGWENLSAVKNGQVFPFDDNLLSRPGPRLVDGLEALAKLLRPELFK"
     gene            15760..16755
                     /locus_tag="PL000_13565"
     CDS             15760..16755
                     /locus_tag="PL000_13565"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNC89978.1"
                     /translation="MRRPFLFSFLFLLAALTLSLAVGSVFISPEELWRVISGTTTNET
                     FRTILIDIRLPRTVLIALVGAALAGSGAAYQGLFRNPLADPYLIGVASGAGLGAVLAM
                     SVKWPYTTLGLLAVPLAAFLASLLTVYLVYIFANVGGTVPTTSLILAGVAFSSFATSL
                     TSFLMLKSTNELRRAIGWLLGGVSLAGWDVILALIPYLAIGLTTLILNGYALNLLQFG
                     DDQAIQMGLNVRRVKVVIIVAASLVTAAAVSFAGIIGFVGLIVPHVVRIWWGVDYRRI
                     LPLSVVGGAGTLLLADVLARVILAPQELPLGIVTALAGAPFFLWVLRRAKNQGIW"
     gene            16775..17305
                     /gene="cobO"
                     /locus_tag="PL000_13570"
     CDS             16775..17305
                     /gene="cobO"
                     /locus_tag="PL000_13570"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC89979.1"
                     /translation="MSKNTKGLLIVNTGDGKGKSTAAFGMVLRAWGRNFRICVIQFIK
                     AETGQWGEIKAAKKLGIEWHATGDGFTWTSKDMDETIARAHHGWEIAKEKIASGNYDL
                     IVLDEFTYTLAYNWLDTNEVMDWLRLNRPSNLHLAITGRSAPQVLIEAADLVTEMTPI
                     KHPYEQGIVAQAGVEF"
     gene            17307..18350
                     /gene="cobT"
                     /locus_tag="PL000_13575"
     CDS             17307..18350
                     /gene="cobT"
                     /locus_tag="PL000_13575"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNC89980.1"
                     /translation="MQLPLIAPLGQLAMQSASERQNRLTKPAGSLGRLEELAIQLAGV
                     TGKANPSIKDKVIITMAGDHGVVEEGVSAYPQEVTPQMVMNFLTGGAAINVLARHIGA
                     RVVVVDMGVANEIPTRDEKLIRRRVALGTKNLAKGPAMTRSQAEESIQSGIEIALAEI
                     AKGADIIGTGDMGIGNTTPSAAIACVFLKQHPKEIAGRGTGVDDAGLARKIATIERGL
                     EVNKPNAEDGIGVLEKVGGFEIGGLAGVMIGAASKQVPVMVDGFISTAAAMIAVSLAP
                     HCHEYLISAHRSKEHGHSLMLEWLGLKPLLDFDMRLGEGTGAALGISMAEAACKILNE
                     MATFEEAGVSSKD"
     assembly_gap    18353..18452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18453..19103
                     /gene="cobS"
                     /locus_tag="PL000_13580"
     CDS             <18453..19103
                     /gene="cobS"
                     /locus_tag="PL000_13580"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNC89981.1"
                     /translation="AVGWFPLVGVVLGFALFGVNYAARLLFPQTVAAALTLFAWIAFT
                     RFFHLDGFMDTLDGLFGGWTPERRLEIMKDSRMGAFGTIGGILVLLTKFAALSEIHAS
                     AMLAAILLATTLGRWASPLVIYAFPYAREDGLGIEMKRNVTFREIIIATLTAGLTAWF
                     TFGWPGFAFMLGAALIGFLAARYAMRLLPGLTGDIYGTVTTLVEMLTLVAFTANLT"
     gene            19121..19729
                     /gene="cobC"
                     /locus_tag="PL000_13585"
     CDS             19121..19729
                     /gene="cobC"
                     /locus_tag="PL000_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HNC89982.1"
                     /translation="MKLYLTRHGQTDWNTAGRYQGQSDTPLNETGLRQAEQIAKRLSK
                     ETIHAIYSSDLSRAANTAQSIADFHALEIKKDSRWRELSFGDWEGMTYQEMSASSPEL
                     FEAWMKDPLTISTPNGETLAQLAERVKAAFNEIKREHKDQTVLVVAHSGSLQSLLSVT
                     LGVDLNRYWQFRISQASLSEMNVYEDSVVLNLLNDISHFQEK"
     gene            19731..20270
                     /gene="cobU"
                     /locus_tag="PL000_13590"
     CDS             19731..20270
                     /gene="cobU"
                     /locus_tag="PL000_13590"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNC89983.1"
                     /translation="MSTLTLILGGARSGKSSHALNLAKESGKPVTFIATAQAFDEEMS
                     ARIQKHKAERPAEWSTLELPMNLASHVNTIRSEVVILDCVTLLATNLMMQFVKDDLVN
                     ETPYLDAVQNETEELLTTIRNTDQNWIIVSNEVGLGLVPPYQMGRVYRDGLGWANQRL
                     AREAETVLFMVAGIPMKVK"
     gene            20304..>21061
                     /locus_tag="PL000_13595"
     CDS             20304..>21061
                     /locus_tag="PL000_13595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89984.1"
                     /translation="MLKLHSLSVSYHDHRVLHDVSFDVNSGEVLALIGPNGAGKSTII
                     RAASGVIPSTGHIRTNGDDFQALSPLQRARYLAVVPQAISLPPAFTVWETVLMGRTPY
                     LGFLGHASTHDEELARQALSRVNALEFAERRVGELSGGEAQRILLARALCQSTPILLL
                     DEPTAHLDLQYQVSLLELIHELAHKENLAVMVALHDLNLAAHFADRVALLVGGHLKAI
                     GTPREVLTSELISQAYCLPVQVVEHPFLGGPLVLP"
BASE COUNT         4892 a         5825 c         5290 g         4754 t
ORIGIN      
        1 atgaaatatc acatctggac cgaaggatgc caaatgaacg ttgccgactc tcagcgggtt
       61 ggctcctcgc ttgagcatct gggttacacc tttaccgaaa ccatcgaaga agccgacgtt
      121 attgttttga acacctgcgt tgtgcgccaa tctgccgaag acaaggcgct tggccgctta
      181 tcatcgctgg ccccgctcaa aaagaaaaac cccaacctcg ttatcaattt aatgggctgc
      241 ctcgttggtg tgcgcggcac cgaaaaattg aaagcccgcc tgccctatgt ggatgtcttc
      301 tccccaccct ctgaccccgg cccattgatc tcgcacctca cccagggaga aatccgctcg
      361 atggaagatg cggaaacaac ccgccgattc ctgatgatgg acgatgaact gatcctcccc
      421 gtggcagaac agggcaaact cgtcactgcc catgtgccga tcgtttacgg ctgctcacat
      481 gcctgcacct tttgcatcat cccctacaag cgcggcgtgg aacgctcccg ccctgtcggc
      541 gacattgtca gcgaagtccg ctcactggca aagcaaggtg taaaagaaat tacattgctc
      601 ggtcaaatcg tagatcgcta cggtaaagac atccccgatg gtcccaacct cgccgcgttg
      661 ctccgcatca ttcacgacga gccaggcgcg gaaggcatcg aacgcatccg cttcctgacc
      721 tcgcacccca actattttga cgacgatctc attcaagcca tcgctgaact cccgcgcgtc
      781 atgccgcaca ttgaactccc cattcaggca ggcgatgatg aagtgctcgc caacatgaaa
      841 cgcggataca cccagcaaaa cttccgcgac ctcgtggaaa aaatccgcgc ccgcattccc
      901 aattgttcca tcgccaccga catcatcgtc ggattccccg gcgaaaccga tgaacaattc
      961 atggaaacct atcgcgtctt atcggacctg aaactggatg tggcgcatct ggcacgctac
     1021 tccgttcgag agggtaccgt cgccacccgc cgcatggaag acaatgtaag cgacgaagaa
     1081 aaaagacgcc gcttccacct gctcgaagaa ttgcaggaac aggtcgtcgg cgagatcaac
     1141 aagaaatatc tcggcgaaac cgtggatgta ctcttcgaag aaaaggtcaa aggccgctgg
     1201 cgcggacgca cccccaccaa caaactggtc ttcgtcgaat cagaagaaga cctgcgcggc
     1261 aaactgcgcc ccgtcaccgt cacatggacg ggaccctggt ccatgcaggc gcagctcgct
     1321 ggggtacgct cgcaaaataa accagaattg atttcgctat aaaaaggtac ggacacggcg
     1381 ttgccgtgtc cgtatgattt tcgggggtta taatttcgcc accatgtctt tcaacgaact
     1441 cattactaca tttgcaaaca acctgctccc catcctgttg gtcagtggag cggggtttat
     1501 tctcggaaaa ttcctcactg tcgattcgcg ctctcttggt cgggtggtct tttacgtatt
     1561 cagccccctg ctcgttctgg atctgatgct caaaagccag ctaaacctgc aacaagccat
     1621 gacgaccgtg gggtacacca tgtcggttat tgccataatg ggtttgctgg ccttcttatt
     1681 cggcaaactc tttaaactgg aaaagcccta tctgctggcg gtcattctta cagtggcctt
     1741 cggcaacaca ggcaattacg gcctgccgct ggtgaagttt gccttcggca ccgatgcatt
     1801 ggcggtcgct tccctgtttt atgtgaccac caccattttg ttcaatacgg tgggtgtggt
     1861 gatcgcttcc atcggccaca tggatctgaa atcggcagtg ctggggttgt tcaaacttcc
     1921 cagtatctac ggggtgacac tggccctcat aataaaaggg ttgggaattc aactccctct
     1981 gcccatttca cgtacaattg agatagccgc aaacggagcc attcccgtca tgcttatttt
     2041 gctcggcttg gaattgaccc gcatcgaatg gtcacacagc ttccgcgcgc tggggttggg
     2101 cgtggcggtg aaacttttac ttggaccgct ggttgggctt gcattggcag ggttgttcaa
     2161 attacagggg catgtgcgtc agggtgccgt attggaagcc gccatgcctg ccgccgttgc
     2221 aaccacggtt gtggcaaccg aatataaatt ggagccatcg ctggtcactg caattgtttt
     2281 tctaggcaca gcgctcagtc cgctgacatt gacgccattg attgtttttc taacgaggta
     2341 aacatgccgc atctaaaaaa actcatcgcc cgattgatgg cgattctact tacagccgcg
     2401 atcctgcctt ttaacttgaa tcaagccggg gcgcagggag gaatcgatgt gactgctgcc
     2461 acggcggtgg tggattttgg tagaacgatc acatttcagg caacgatcac agctccgatc
     2521 ggcattcaac aggtttctct tctcttccgc ggtatgaatg aagatgtgac tcgggtggag
     2581 cccgtgcagc ttgggcctga tggatttgtg agtttcactt acgatgcctc gcgcaatttt
     2641 ttcccgccgt ttagttcggt cgtattctgg tttcaagcca ctttggccga taaccaaacc
     2701 tacaccagcc agcccatcac ctttgcttac aacgataatc gttttccatg gcgtgaggca
     2761 aggagagata accttgtggt gcattggtat gcgggcgatg atgcttttgg cgccgccgca
     2821 ttggatgccg ccaacagggg catcctttcc atcaatgagt tcattcccat ttcttcaagc
     2881 cagcccatcg atatttacat ctattcaaat gcactcgacc tgcaaaatac gctcttgctt
     2941 ggcggccagg aatgggtcgg cggacatgcc aacccggagt tgggcgtggt gctggttgcc
     3001 atttcccccg gcgtcaatca atccattgaa cttgaaacca tgatcccgca cgaacttgcg
     3061 catgtgatgt tgtaccgttc gctgggaggg acttacaaca ttcaaccgct ctggctgctt
     3121 gaaggcatcg ccaccatggc ggaatatccc aaccctgatt acaaacatgc gctggatatt
     3181 gccagcggaa ataatacctt gctctctttt gcagacttat gcgtttcctt ccccgccgac
     3241 gcaggcaacg cctatctggc ttatgctcaa tcccaatcat ttgtcaccta catccgcgaa
     3301 acctacggca cctcaggcct ggcacgtttg accaaatcat acagcgaagg ctttaactgc
     3361 gaactcggtg caaccaacgc ccttggcacc ccattgagcc tgctggatgt gcgctggcgc
     3421 gaaagtgtgc ttggacaaaa cgtcgcaggt gtggcttttc ggaatctttc accttatctg
     3481 ttgttgatgg ctctggtgtt ggtcgttccc atgtggggcg cgattgatat gctgcgccaa
     3541 aggagaaaac gtgcaaaaca atccgactga cgaattgaaa cgcgtacaca tgtgggtcaa
     3601 gggacgggtg caaggggtgg gcttccgtgc gcatgtggag taccatgccc tgcagatcgg
     3661 tgtgcttggc tgggtgagaa acctcggtca tgatacggtt gaaaccgtgg cagaaggcac
     3721 caaagaacag attgctcaat ttatcgagat ggtgaaaaca ggtccgcggc actcacgagt
     3781 ggacgaagct cgcgtggaat atgaacccac caccggacat ctaagcggtt tcaccgtaaa
     3841 gatgagcatg taacatcacg aaaaccccaa ccctctttcc ttcaacgaaa cccatctccc
     3901 cagtccgatc accaaatgat cgagcacatc cacatccaac agctttcctg cctgcaccat
     3961 ggcgcgtgtc acggctacat catcggggct gggagtcgga tcgccgcttg ggtggttatg
     4021 cgcaactata atcgcggatg cattggctcg aatcgcatct ttgaacaact cccccacacg
     4081 aacctgtgat gaattgaccg agcctttata aacttccaca gtttccaaga cccgattcct
     4141 tctgtccaac aaaagcaccc gcagatgttc ctgctccagc gccgacattt caaattgcaa
     4201 caatgcggcg gcatctgcgg gactattgat ggaaggccgt tcttcagggg cttccaaagt
     4261 caaccttctt cccaattcaa tcgcggcttt gatctgtgcg gccttggcct ccccaatggc
     4321 atgttgatcc atgagttctt tgatcggtgc gcggtgcaac ccactgagcc cgccaaattt
     4381 tttcaacaaa cgctgcccta cttcaacggc attttcacca accacaccca cacgcagcaa
     4441 aatggcaagg agttcggcac ttgtcaacgc ttgcggcccc agcgccacca atctttcacg
     4501 cggacggtcc gattcgtgca agtctgcaat gcgatacaca ggtttcgaat tgttttctgt
     4561 catggccact ccttggtttg gtgcatgcaa ttttacaaca aaaaaaagcc ccatctttcg
     4621 acggggcttt tttttgaaaa ggttgggatt acacgttgct gaggctggag ttcactttgc
     4681 tgaacacgtt gccgatgatg ggtccgagga tcatcaaagc cgcaataacg acgatagcta
     4741 ccaaaacgag gatcagcgca tattcaacga ggccctgacc tttttcttta ggggcgaata
     4801 acatggatgt atttctcctt tcataaaata tgctcaggaa cgcttcctga attactttta
     4861 ttttacccac tccagaaaaa aacacaagag aaaaacgatt gcatcttctt aacagttatg
     4921 ttagtcattt atactttatt gtatgctatc ctcgcaaaaa gcaggacaag gacagtttca
     4981 tatcatcccc ccgcaacaaa cttttctgtg gttaaattgc gcaacataaa tgagccaggt
     5041 gcgaacaact tatctccacg tgcatggctg cgggaacgtt tatgaaaaca gcctactatt
     5101 gaaagaatcg gcggtcatca tgttaattca ttcagcatca caacttctca cccttgcagg
     5161 cggcccgcaa cgcggacatg ccctcggcac acttggcatc attgaaaatg gcgctgtcat
     5221 tgtgcgcgat gaaaagatcg tcgcagtcgg caccaccgaa gaactcaaaa atgcctaccc
     5281 cgatgaaccc acactggatg ccgggcaatc ggtgctgatg cccggatttg tagacccgca
     5341 cacacatgtc atttgggcgg gtgatcgagc caacgaattc gaaatgaaaa tgggcggcat
     5401 ggcctacctc gatatccttg cggcaggcgg aggcatcatc tccaccgtca aagccacacg
     5461 caccgccagc ctcgaaacgc tcatcgcaca aacccgtcca cgcctgttga aaatgttctc
     5521 gcatggcacc accaccgccg aagcgaaaac cgggtacggt ctgcaaactt ccactgaatt
     5581 gcgcctgctc aaagcactga tcgcgcttga cgatgaaagt ccgctcgacc ttgccatcac
     5641 cttccttggc gcgcacgcca ttgcccccga atacaaagac aacccgcaag ggtacactga
     5701 tgaagtcgtc aacaccatgt tgccgatgtt aaaggaatgg tgggaaacac atgccccccg
     5761 cctgcccctg ccttttgtgg atgtcttctg cgaaaccaaa gccttcgacc ttgaacagtc
     5821 gcgtcagatc ttaacccggg ctgggtcttt gggattcccg ctcaagatcc acgccgacga
     5881 attcgacaac ctcggcggcg cagcgctcgc cgcagagttg ggtgccgcct ctgccgacca
     5941 tctcgtacgc acatccgatg cagatatcgc cgccctcggc aaatcagata cggtggctgt
     6001 agccctgcca tgcacaccct ttggtttggc agaaaaagat tacacccccg ccagaaaact
     6061 catcgaagcc gatgccatgc tggctctggc cactgattgc aacccaggca cctcgtggaa
     6121 cgagtccatg caatttgcga ttgcgctggc ctgccgctac atgaaattga cccccgcaca
     6181 agccatcgcc gccgccacta ttaattccgc ccacgccatc cgcaggtccg actcgatcgg
     6241 ctccatcgag gtgggcaagc aagccgatat gctcatctta accgtgccgg attatcgcca
     6301 agtgggatat cgctacggca caaaccttgt caaacaagtc atcaagcgcg gacgtgtcta
     6361 ttcggtggat gttggttact acagaaccgc ataacgaagg aaatttatgg atatccgttg
     6421 gatcattttt ggggtcattc tagtcatcaa ttcgatcttc gccatcacga cgatcgtcct
     6481 catcacaagg aaatttgatg tcgccgggcg cgatgcatta atcatgatgc tttgctccct
     6541 ggcgatctgg tcttttgcct atgcaatgat caccttctcc agcgatctgg agaccaagaa
     6601 gttctggctg cgggtggaga atgtcggaat tctttcacag cctgtttttt ggatcgtttt
     6661 cattctgggg tattcacgac agggacgtgc cttgaattgg ggttatattg ccctgctatc
     6721 cattgtcccc gcagtatcca tgatcatgat cgcctccgac cgctggttcc atctctatta
     6781 ctcatcggtg aaattgcttg atggaaatta cggcccactc gtcatcgagc gcggaccctg
     6841 gtatggggtt gcggtttttc aggcctatgc acttgacctg atcgccacca tcctgctcat
     6901 ctggcggttt attcaattca gaaatatctt tcgcacacaa cttgttttcc tcattgtggc
     6961 ggccttgatt cccctgttca caaacatcgg ctatcaggtt gtatccattt ttttaccgtc
     7021 gttattcctc ccagtggatc tgaccccgat ttcatttacc ctcagcatca gcctgatcag
     7081 ttcaggtatt ttccgtaccc gcctgctcga tctcgtccca attgcgcgcg atgtggtcat
     7141 ggagcacatt cctgaaatgg tgttcgttgt agatgctcac gatcgtgtgc tggatgtaaa
     7201 caccgtggct gaaaaatggc tggggatgac ccgtgaccag atcattggca aagaccccat
     7261 cgaaattttc gggaaatggc ccgatctgta caaccgtttt ctcaccacag aaaagactcg
     7321 tgaagaagtc aaaatttatg gcgacccgcc tttgaccctt gaagtggtca ttaccccgtt
     7381 gtataacaac gaaaaaatta acgggcgtgt cgttcttgcc tacgacatca ccaagcgcaa
     7441 acaactcgaa ctcgacttga aggcaaagat tattgagatc gattcgctca gggttcagct
     7501 tcaagaccag gccatccgcg actcgttgac cggtgtgttc aatcgccgtt ttcttgcgga
     7561 agcgcttgaa agggagatcg caaaggccaa ccgtgaagac atgcccatct ccatcgtcat
     7621 gatggatgtg gatcatttca agtcattcaa cgatacctat ggacataaat gcggtgatgc
     7681 cgtattgcgc gacctggcgg gcttcctgac caaaaactcg cggcatggag atattgtctg
     7741 ccgatatggc ggcgaggaat ttgtcattct catgccggaa gcctcttttc aaaacgcatt
     7801 cgaacgggcg caaagctggc agcaggccta tgcaagtaaa accattgaat atgaagataa
     7861 agccctgaaa gtaaccttct ctgccggtgt agcaagttat ccgcagcacg gacccaatgg
     7921 tgaaaccatc ctccatgctg ccgaccacgc cttgtatgaa tccaaaagca atggccgcaa
     7981 ccgtgtcact gcttttcaaa aacgccttac aaaggagaat taaccatgca aatcgacatt
     8041 atcggtgtac ctattgacct cggcgcagac cgtcgcggtg tggatatggg ccccagcgca
     8101 atccgctatg cacacctgca aaagaaactg gaagatcttg gctatgacgt ccacgatgaa
     8161 ggcaacgtgg aagttcccat cgcagaaatg tgcaagatca ccaacccgaa attgaaatat
     8221 attgattgca tcattcccat gtcgcgccgt gtggcgggcg cagtggcaac ctccatccag
     8281 tccaaaaatt ttccgctggt attgggcggc gaccacagcc tctccattgg ttcggtccgc
     8341 ggcgctgcac gcaacaaaaa gatcggcgtc atctggatag acgcccacgc cgatttcaac
     8401 accgccgaaa ccacaccctc aggcaacatc cacggcatgt cgcttgcagt tcttgccggg
     8461 ctgggtgata aaagccttgt gcaattatgg gatgaaaaca tcccagtcat tgacccatcg
     8521 aagatcgcca tcatcggcgc acgtgacctt gactcgggtg aaaaggtcaa cttgagtaat
     8581 gcaggcgcca tggtaatggg catggaacag atcgaccgct atggaatggt ctcggtggtg
     8641 gaaaaggcca tcgaacgagt cagccgtgat gtggatggga tctacctctc cctcgacctc
     8701 gattcgctcg acccggaaca tgctcccggt gtagggactc ccgtctcagg ggggctgacc
     8761 cagcgcgaaa cccacctcgc ctgcgaattg atcgccgaaa caggcaaact catcggcatg
     8821 gatgtggtgg aagtcaacgc cattctcgat gtgcaaaatc gaactgcctc cctcgccgta
     8881 gacctggccc tctctgcact cggccgccgc atttggaatg gacaatcatg aagccaacgc
     8941 tgaaagaagt cgaacaactg gctcggggcg caggtgccat tctgcgtgaa cgctacaacc
     9001 aggaacatga gatcagctat aaaggcgtga tcgatctcgt caccgaagcc gaccacgcct
     9061 cggaagcctt tttgatcggc gagatccaat ccaaatttcc aggcagccat atccttgctg
     9121 aggaaagcgg cgaaaccaaa ggcagcaatg aaggcgtctg gtacattgac cccttggacg
     9181 ggaccgtgaa ttttgcacat cacattcctg tattttgcgt ttcgattgcg tatgcctttg
     9241 aaggcagcat gctgcttgca gccgtgtacg acccactacg cgatgaaatg ttcactgccg
     9301 aacgcggcaa gggtgccttc ctcaacggaa agccgatcca tgccgccaaa acgaccgaac
     9361 tgcaaagaag cctgctagta acagggtttc catacgatac gtggaatact gaaaaagata
     9421 acttcaaaaa tttcgagaaa cttgccaaga tgacccaagg tgtgcgccga ctcggttctg
     9481 ccgccctcga ttgctcatac gtcggtgctg gccgtttcga cggcttttgg gaactcaccc
     9541 tcaggccgtg ggatatcgcc gccgctggtc tcattgccga ggaggcagga gcaaaagtca
     9601 cagcaacaga tggcaagcca gattatattt ccgcaccaca atccattttg gcgaccgcgc
     9661 cggggattta tcagcaaatg cttgaacagt tgaattaaga gaaattcnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnntta ttgatacgga ccatcgccag gagacccgcc
     9841 gggtaacgcc agccaattgg cttcggaatc tggttggcgg gggtcaatac gttccgcgga
     9901 ttcacactca ctgagatgga gcggtgtgtg gtcaaggtga ttatcgtcaa tgttgaccgg
     9961 gtaggggcca ttgatggtgg gcgtaccgta ggtatccact acaaggtcca tgacggtgtc
    10021 actgccaatg taaagtgtga ccgtgtcgta accatcctga tcgctgatga tgccaaaata
    10081 atcatcgccg agggtctggc ttgattcggc ggttaggatg accacaccgg gcggaaattt
    10141 atacggcatc cgattttcga tattgccttc gggatacaga ggggaaagaa caaccgcaaa
    10201 gctgcggggc gggatcaccg tcgagttggt gacaagtgtc gggtcgagtt gaagcgatga
    10261 tcttgaatcc catgcaacga gtcggtcagg agtccctttt tcgccttcgt cgaacagcat
    10321 ccaactgccg acatccacag gcagatcgcc gaagttgtaa agctccacat attgattgaa
    10381 cttgcgcgtt tcgagtccgc cgcattgatt ccccatcact tcgctaatgg caatggtgat
    10441 tgccgcaggg tcgttgacag tgggcgcgct ggcagtggag tcttgttcgg tgacggcaca
    10501 ggcgcggctg gcagtgtaag gggcgggagt gcctgaaacg aaaagaaccg agatcacagc
    10561 ttcaccgggc aagttcgggg cgagaaaagt ggcggtcaac ccctgccccg tccaaaccac
    10621 ttcacctgtt gaagcactcc attgaatttg agtgttcgca ggaatctgtc catcaagggt
    10681 aagtgccagt tgaccgcccg cctgcacccg acagtcatcg ggcagaatgg atgcgttgag
    10741 attggagtta tgccccggcg aaccgcaggc gttcagccac aaggaagaaa aaatgaaaag
    10801 gcatgcggtg agttttttca tagatcaggg agttccaatg actggctgca atgagaagat
    10861 ggatgtttgt gaacagaaat tttcagaaac gctgacggtc ataaacccac tgccggtctg
    10921 ctggttgaat tttgcactga cctgacagcc gcgctgtgcg ccaatggttt cgaagatgcc
    10981 tccggcatat tcccacgaac aagacagggt ggtatctgta ttgacggaat gtgcggtgac
    11041 tgtgacaacc tcaccgggtg aaatgggaac acggtcggat ggttgaaaaa aaccttccct
    11101 gcctgaaatt tcaaattgaa tggtcacggg cggctggcag gatggaaaca cgttccaaaa
    11161 ggcgatcaat gaccccgccc ataacaatcc cagcgaccac agcggcaacc ctgaaagata
    11221 aggctggagc ttttcagaag atgtgaccat tttgatgcgt ggaatttcaa tgccgaaccg
    11281 cagaaaaaca aggatcgtag cgctgaatgc catgctgaaa taagccagcg cattttgcgg
    11341 attgtggctt tgcccgagcc agttaatgaa ctgtacaggc accaccgaaa gccgcagaat
    11401 gacagcaaac gcgatgagca gggtcaacac aatgtacacc gccagaccgg aaatgaactc
    11461 aaggggtttt ctgctcatgt ttctccacgc gttgagatta tagtttttcc gcatttgggt
    11521 caactttcca aatcctttac agttggcatt ccgggcggct ggcgtataat aaaagttaca
    11581 tggaaaccaa aaaacttatc ctcgttacag gtgccacagg ctatgtaggc ggaagactgg
    11641 tacccaaact gcttgaagcg ggttatcacg tgcgctgcct tgtacgtgac ccatcccgtt
    11701 tgcagggacg ttcctggctg aaaagagtgg aagtcatttc cggagatgca ctttcacccg
    11761 aagggcttgc cgcagccatg cagggcgttt cagcagcgta ttacctgatc cacgggcagc
    11821 agggtggaaa gatcgatgcg gaacgtgatt tgcaagtggc gcgtaatttc gcccaggccg
    11881 ctgaagatgc gaacattgaa cgcattattt atctgggtga attggtggac ccgaccgcca
    11941 acctctctcc ctatttgcgt gcccgccatg aaacaggcta cattctgcgc tatggcagtg
    12001 tgcccgtcac cgaatttcgc gcaggcatga tcgtcggttc gggaagtgcc ctttttgaaa
    12061 tgatccgcta cctcgtcgag cgtgaaccgt tgttgatctg tcccgcatgg tttttttcac
    12121 aggcacaacc catcgcgatc cgtgatgtgc tgtcgtatct ggtggatgcg ctcaaaacgc
    12181 ccgacagcaa cggccgcgtg atcgaaattg gcggccccag ccgactcacc tatgccgata
    12241 tgcttttgaa ttatgcaaaa gaacgcagcc tgaaacgctg gttgatcccc acaccattca
    12301 acgcgccgcg gctttcggcg tactgggttc acatgatcac gccgatccat tggcgcgtgg
    12361 tggcaccgct cattgagggt ttgcgcgcac ggctgattgt ccgcgatgat acggcaagaa
    12421 aactttttcc gcagatcaag ccgatcgatt ttcaaaccgc cgcgcatctg gcgcttggac
    12481 gcattcaacg cgataacgtg gaaacaagct gggctgatgc actggtcacc tccacgggcg
    12541 acatcaaacc gtatgaattc actgttgaag aaggcatgtt catcgaacgc cgacagatcc
    12601 tgcttgacct caaacccgag tcggtcttcc gcgcttactc tggaatcggc ggcgaacgcg
    12661 gctggcttta tatggattgg tcatgggcca tccgtggctg gatggataaa gcaatcggcg
    12721 gggtgggctt gcgccgcggc cgccgccacc ccgatgaaat tcacaccggc gaatcgcttg
    12781 atttctggcg cgtggaaaac gtggaaaaga atcggctcat gcgcctgcgc gctgaaatga
    12841 tcgtccctgg caaagcctgg ctgcaattcg aatccacccc gcagggaaca caggaagaga
    12901 aaaccctgct cacggtcaca gcgtattttg caccgcacgg ggttccgggc tttttgtatt
    12961 ggtacgccat gtggcccttc cacaaattca tcttcgacgg ccttgcccgt caactcgcct
    13021 cccgggcacg ggtgttgagt cattcgtatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn acacctcgat gaagccactt ttgatccttg tcaccggcgc aactggatac
    13201 attgccagca gattgattcc ccaactgctg gatcttggcc atacggtccg cgcactggca
    13261 aggcgcccgc atcaaatcgg acctcgaaac tggtctgccc acattgacat tttccccggc
    13321 gatgtgacca accccgcctc ccttgcaccc gcattgaagg acgtccacac ggcttactac
    13381 ctcattcaca acatgtcttc ggggcatggc tataccgata ttgagttaca agccgcacgc
    13441 aattttgcca gggctgccga agaagcgggg gtcaaacaca tcatctacct tggcggtctt
    13501 gccgaccccg aacaacacat tgcgccgcac atgcgttcac gcattgaaac aggcgccacc
    13561 ctgcgcgcag ggaaagtacc cgtcaccgaa tttcgagcag gtgtgatcgc aggttcaggg
    13621 agcatttcct ttgaaatgat ccgtttcatg accgaattgt ttcccatcat ccccgcccct
    13681 gcctggatcc aaaacaaatc ccaacccatc gcagcccaaa acgtgatcga ttatctgctc
    13741 gctgccctcg aaaactggga cggtcatggc aaggtcttcg agatcggcgg tcccgacatc
    13801 acaacctatc aagacctcat gctgcgttat gcccacatcc gcggatataa acgcagtttc
    13861 ctcgttctgc cctacgtccc tgtctggttt atggcttttg gcatcgggct gatgaccccc
    13921 gttccgcgcc cgattgccca tgcactggtc ggcgggctat ccagtgacag tgttgtcatg
    13981 cacgatcacg cccgcgcggt ttactcaaaa atcaaactga ttgatttcga ttccgccgcc
    14041 aaagaagcgc tgacccatct gcatccgcac cgcatcgaac gtgtgtggga tgacggcaca
    14101 ggccgctcca aaacgctcaa gcacgaaggt tttttcatcg cccaccacga ggccaaaacg
    14161 aacgccgcac ctgaacagat cttcactgcc atcaaaaaca tgaaatggca ggaattcgtc
    14221 tttgattcca tcgaagagaa tcgcatcctt ctgttgcggt cgaaacatac atcggttggc
    14281 cccatgttcc tcgaatggcg gctgacgaat caaaatcaca tcgccctgac ctattacctg
    14341 gccccacgcg gcccgcttgg atttttatac tggcatttgt tgtatccgct ccacacctac
    14401 caactgcgca agttgttaaa acgcgtcgtc cgcaactcag tggtaaaatg aaaacataaa
    14461 taacgatcgg caagcggcga agctggtgag attccagcac tgtcgcgcaa ctgtgaagtt
    14521 agtcatgttg tctctttgcc ttctcgtcta gtagacaaga agaccagcag acgaaaagac
    14581 taatgaagtc aggtcgcccg ctactgatcg gttcaccaca ctctcgcgaa aaggaggtgg
    14641 atgacggtag cctgatcgga ttgcctctcc cctccccagc ccatgctggg gattttgatt
    14701 cccgtggtaa caaaaatctc aaggagaaaa aatgtttcga aaaattttag ttctggcttt
    14761 actactcgcg ctcttcatca caggatgcgg ccccgcacgc tccgcgacac cgaccgaggt
    14821 gcctgctcct gtttcaacag aaattcccgc cactgaagca ccctcttcgt cagcggagat
    14881 tcaactgaca gatgggctgg gcaggtctgt ggtcctgaaa gaagcggcgc agcgcatcat
    14941 ctcgctggca ccgtcaaata ccgaaatcct cttcgccatc ggcgctggtt cacaaatggt
    15001 tggccgcgat tcgctttccg attatcccgc cgaatcagcc aacgtaaccg atattggttc
    15061 ggcatacgaa gccttgaaca cagaaatgat catctcgctc aagcctgatc tgatcatcgc
    15121 cgccgagatc aacacacccg aacaggtaaa agaactcgaa gacctcgggc tgaccgtgta
    15181 ttacttgaac aacccccaca cgctggagga actgtacgac aatcttcaga ttgtcgcaca
    15241 gttaaccggg cgcgaagcag aaaccaccgc actgatcgat tctctcaaag cgcgcgttgc
    15301 agctgtggat gaaaagattg cgccgctcag ttcacgcatc agcatttttt atgaaattga
    15361 tggcaccgac cctgccaaac cctacaccgc aggcaaggac actttcatca ccctgttgat
    15421 cgagcgcgca ggcggataca acatcgctgc agatatcgaa ggctacccac aattgagtct
    15481 cgaacaagtc gtcgccgcgg accctgcctt tattttgctg ggcgatgcag cctatggcgt
    15541 tacccccgaa tcaattgcca cccgcccagg ctgggagaat ttatccgcag tgaaaaatgg
    15601 acaggtcttc cccttcgacg ataacctgct cagccgcccc ggtccgcgtt tggtggatgg
    15661 gcttgaagcc ttggcaaaac tgttgaggcc tgagttattt aagtagaacg taaactggta
    15721 attggtaatt gctttttacc aattaccaac ctcccatcca tgcgccgtcc gtttcttttc
    15781 tcttttctct tccttctagc cgccctgacc ctcagtcttg cggttggttc tgttttcatt
    15841 tccccggaag agttatggcg tgtcatcagc ggcacgacca cgaacgaaac ctttcgcacc
    15901 attctgatcg atattcgtct gccgcgcacg gttttgattg cgctggtcgg cgcggctctg
    15961 gcaggcagcg gcgcggcata tcagggactg ttccgcaacc cgctggctga cccgtatttg
    16021 atcggcgtgg cttcaggtgc agggctgggc gcggtgttgg cgatgtcggt caaatggccg
    16081 tacaccacgc ttggattact ggccgttccg cttgcggcat ttctcgccag cctgctgacg
    16141 gtgtacctcg tctatatttt tgcgaatgtg ggcggtacgg tgccaactac aagtttgatc
    16201 ctcgcggggg tggcgttctc atcctttgcc acatcactga cctcattttt gatgctgaag
    16261 tcaaccaatg aactgcgccg tgccatcggc tggttgttgg gtggggtgag tttggcaggc
    16321 tgggatgtga tcctcgcgct catcccctat ctcgccatcg gcttgaccac attgatcttg
    16381 aacgggtacg cgttgaatct tttgcaattc ggcgacgacc aggccataca gatgggtttg
    16441 aatgtgcgcc gtgtgaaagt cgtcatcatc gtggcggcat cgctggtcac agcggcggcg
    16501 gtttcgtttg cgggcatcat cggctttgtt gggctgatcg ttccgcatgt ggtgagaatc
    16561 tggtggggcg tggattatcg ccgcatcctt ccgctcagtg tcgtgggcgg tgcaggcacc
    16621 ttactgctgg ccgatgtgct ggcacgggtg attcttgcgc cgcaggaatt gccgctgggc
    16681 attgtcacag cattggcagg cgcacctttc tttttatggg tgttgcgccg cgcaaaaaat
    16741 caaggcatct ggtaacactt catcaaggaa tcatatgagc aaaaacacaa aaggattatt
    16801 gatcgtcaac acaggcgacg gcaagggcaa atccaccgcc gccttcggca tggtgctgcg
    16861 tgcatgggga cgcaactttc gcatttgcgt catccaattc attaaagccg agacggggca
    16921 atggggcgag atcaaagccg cgaaaaaact cggcatcgaa tggcatgcca caggcgacgg
    16981 ttttacatgg acatcaaagg atatggatga aaccatcgcg cgcgcccatc atggctggga
    17041 gatcgccaaa gaaaagatcg ccagcggaaa ttatgacctg atcgtgctgg atgaatttac
    17101 ctacaccctc gcctacaact ggctcgacac aaacgaagtg atggactggc tgcgcctcaa
    17161 ccgaccctcg aacctgcatc tggccatcac aggcaggtca gcgccgcagg ttttgatcga
    17221 agccgccgac cttgtcaccg agatgacccc gatcaagcat ccatacgaac agggcattgt
    17281 tgcccaggca ggagttgaat tctaaaatgc agcttcctct cattgcccca ctcggacagc
    17341 ttgcaatgca atctgcgagc gaacgccaga acagactcac caaacccgca ggcagtttgg
    17401 gacggctgga agaattggcc attcaactgg ctggtgtcac tggcaaggca aatccatcca
    17461 tcaaagacaa ggtcatcatc acaatggctg gcgatcatgg cgttgttgaa gaaggtgtca
    17521 gcgcgtaccc acaggaggtg accccgcaaa tggtgatgaa tttcctcacg ggcggcgcag
    17581 cgatcaatgt gctggcaagg catatcggtg cgcgggtcgt ggtggtggat atgggtgttg
    17641 caaacgagat cccaaccagg gatgaaaaac tgattcggcg ccgagtggct ttgggaacga
    17701 agaatctggc caagggtcct gcaatgactc gttcacaggc ggaagaatcc attcaaagcg
    17761 gaatcgaaat cgctttggca gagatcgcaa aaggcgcaga catcatcggc acaggtgaca
    17821 tgggcattgg caataccacg ccttcggcag ccattgcctg cgtctttttg aaacaacatc
    17881 cgaaagagat tgcaggccgc ggcacaggcg tggacgatgc agggcttgcc agaaaaattg
    17941 cgacgatcga gcgcgggctg gaagtaaaca agcccaacgc agaggatggc attggcgtgt
    18001 tggaaaaagt gggcggtttt gagatcggcg ggctggcggg ggtgatgatc ggtgccgctt
    18061 cgaaacaggt gcctgtgatg gtggatgggt ttatctccac tgccgccgca atgatcgcgg
    18121 tttcgcttgc gccgcattgc cacgagtatt tgatctccgc ccatcgttca aaggaacacg
    18181 gacattcgct catgttggaa tggctcggtt tgaaaccgct gctcgatttc gatatgcgtc
    18241 tcggtgaagg cactggcgca gcgcttggca tttcaatggc agaagctgca tgcaagatac
    18301 tgaacgaaat ggccaccttt gaagaagcag gtgttagcag taaagattaa ccnnnnnnnn
    18361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgtggg ctggtttccg cttgtgggcg
    18481 tggtgctggg atttgcctta ttcggtgtga attacgcggc acggctgctt tttccgcaaa
    18541 ccgtcgcggc tgcattgacc ctgttcgcat ggatcgcgtt cacgcgtttc tttcaccttg
    18601 atggtttcat ggataccctc gatggtttgt tcggcggctg gacccccgaa cgcagactcg
    18661 agatcatgaa agattcacgc atgggcgcct ttggaacgat cggcggcatt ttggttttgt
    18721 tgaccaaatt tgcggcgctt tccgaaatcc atgcctcggc catgttggct gcgatcctcc
    18781 tcgccactac gctgggacgc tgggcttcac cacttgtcat ttacgccttt ccctacgcgc
    18841 gcgaagacgg gctgggaatt gagatgaaac gcaatgtgac tttccgtgag atcatcattg
    18901 ccacactgac agcagggctc actgcatggt tcacgtttgg ctggcctggg tttgccttca
    18961 tgctcggcgc ggcactgatc ggcttcctcg ccgcccgcta tgccatgcgt ctgctccccg
    19021 ggctgacggg cgatatctac ggcacagtca ccacgctggt cgaaatgctg accctcgttg
    19081 catttactgc caacctcacc tgaaacctgg aacctgaaac ttgaaactct atctcacccg
    19141 ccacggacaa acagactgga acaccgcagg ccgttatcag gggcagagcg atacgccact
    19201 gaacgagact ggtctgcgtc aggctgaaca gatcgcaaaa cggctctcaa aagaaaccat
    19261 ccacgccatt tactccagtg acctttcacg tgccgcaaac actgcccaat ccatcgcaga
    19321 ttttcacgca ctggaaatca aaaaggattc ccgctggcgc gaactttcct tcggcgattg
    19381 ggaaggcatg acctatcagg aaatgtcggc atcttcgccc gaattgttcg aagcatggat
    19441 gaaagaccca ctcacaattt ccacgccaaa cggcgaaacc cttgcccaat tggcagagcg
    19501 ggtgaaggcg gccttcaatg aaatcaagcg cgaacacaaa gaccagaccg ttctggtcgt
    19561 ggcgcacagc ggatcattac aatccctgct gtcggtcacc cttggcgtgg acttgaaccg
    19621 ctactggcaa ttccgcattt cgcaggcatc gctctctgaa atgaatgtat atgaagatag
    19681 tgttgtactg aacttactca atgacatatc gcattttcag gaaaaataac atgagcaccc
    19741 tcaccctcat cctcggcgga gcacgcagcg ggaagtcatc gcatgcgctg aacctggcaa
    19801 aagaatcggg aaagcccgtc actttcattg ccaccgcaca ggcatttgac gaggaaatgt
    19861 cagcacggat tcaaaagcac aaggcggagc gccccgccga atggtccacg cttgaactgc
    19921 cgatgaatct cgccagccat gtgaacacga tccgctcgga agttgtcatc ctcgattgtg
    19981 taaccttgct ggcgaccaac ctgatgatgc aatttgtcaa agatgatctg gtgaacgaaa
    20041 cgccctatct ggacgccgtt caaaatgaaa ccgaagaact gcttacgacc atccgaaaca
    20101 ccgaccaaaa ctggatcatc gtttcaaatg aagtcggcct tgggcttgtc ccaccctatc
    20161 aaatggggcg cgtttaccgc gacggactcg gctgggccaa tcaacgcctc gcccgggaag
    20221 ccgaaacagt gctcttcatg gtggcaggta ttcccatgaa ggtcaaataa cccattttcc
    20281 actttcatcc atttcgagtt cccatgctaa aactccactc cctctccgtt tcctaccatg
    20341 accaccgcgt tctgcacgac gtttctttcg atgtgaacag cggtgaagtg ctcgcgctca
    20401 tcggaccgaa cggcgcgggc aaatccacca tcatccgtgc agcgagcgga gtgattccct
    20461 ccacggggca catccgcacc aatggcgatg acttccaagc cctctcccct cttcaacgcg
    20521 cacgctatct tgccgtggtt ccgcaggcga tctccctgcc gcccgccttt acagtttggg
    20581 aaacggtttt gatgggacgc acaccctatc tcggattcct cggccacgct tccacacatg
    20641 acgaggaact ggcacggcaa gccctgagca gagtcaacgc cttggaattt gccgagcgcc
    20701 gcgtcggaga attatcaggc ggcgaagccc agcggattct gctggcacgt gccctgtgcc
    20761 aatccacacc gatccttttg ctcgatgaac ccaccgccca tctggatctg caatatcaag
    20821 tcagtttgtt ggaattgatc cacgaactcg cccacaagga aaacctcgcc gtgatggtgg
    20881 ccctgcacga cctcaacctt gccgcccatt tcgccgaccg cgtagccctg ctcgtcggcg
    20941 gtcacctcaa agccattggc acaccccgcg aagtactgac ctccgaactg atctcccaag
    21001 cctattgcct ccccgttcaa gtggtggagc atccattttt aggcgggccg ctggtgctgc
    21061 c
//
LOCUS       DAOQLC010000076        20691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_892_length_20691_cov_19.731440, whole genome shotgun sequence.
ACCESSION   DAOQLC010000076 DAOQLC010000000
VERSION     DAOQLC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_20691_cov_19.731440"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PL000_13600"
     CDS             complement(<1..305)
                     /locus_tag="PL000_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89985.1"
                     /translation="MTETTFTQKVPALPAWKVIFEMVRFRPWLWVVDLFSVALIRFCW
                     QVAPALIIKAFFDMVTGEAQLTFGIWAIVAFLAATWIGRVVASYGFYYADVPIFAEM"
     gene            complement(394..2139)
                     /locus_tag="PL000_13605"
     CDS             complement(394..2139)
                     /locus_tag="PL000_13605"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC89986.1"
                     /translation="MNLSFKSYWNLLSDHIRPQKGRFILLAALLFGSIGLRIFAPQIM
                     RRFIDSALAGEALSTLMWTAIAFIGIALIQQAVAVSVTYLGENVAWTATNDLRAELAE
                     HALHLDMKFHNDHTPGELIERIDGDVTELATFFSQFALNLIANGLLLVGILVALFLED
                     WRAGLAFAVYSILTIFVLGRLKDIAVPHQKARREADAQIFGFIEEQLAGTEDIRSSGA
                     VDYSIRELFRLQGIKLTHDRKAHFKRWIIENAMGFALTTGNLLAISSGYWLFSAGLVT
                     IGTVYLFVHYINLLEEPFWAMTHEIESFQTIGACVERLTEFRNFKAEVVSGVGVEIPA
                     RPLELTFEDVTFSYNGEDTVINGLSFDLRAGSVLGLLGRTGSGKTTLGRLIFRLYDVN
                     AGSIKVNGADLRDAHIETLRRNIAIVTQDVQLFRASIRDNLTFFDRTISDEKIIATLE
                     ELELGDWYQNLPKGLDTELETGSRSLSAGEAQLLAFTRVFLRNPGLVILDEASSRLDP
                     ATEQKLERAIDKLLKNRTAIIIAHRLDTIERADNVMILDKGKVSEYGNRKELAADLNS
                     RFYQLLQTGMEEVLA"
     gene            2446..4134
                     /locus_tag="PL000_13610"
     CDS             2446..4134
                     /locus_tag="PL000_13610"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNC89987.1"
                     /translation="MKNTYRMRYWRIVLFFARVTLSIIFWDIFLPRIGLRFLSRNTRP
                     QRLRGIAVRFRALAIRLGGVMIKVGQFLSARLDVLPPEITDELAGLQDEVPPVDYEAI
                     RVQTELELGSALEKIFLTFEKAPIAAASLGQVHRARLFPHEAETIGFENIVVKVLRPN
                     IEQIVDVDMSALRVVGGWLKRYRPVSDRADVPAIVEEFAATIREEVDYLSEGKNAEAF
                     AINFKGDENVYVPRVVWTRSSRRVLALEDVSAIKITDYEAITAAGISRAAVADRLLAT
                     YLKQIFEDGFFHADPHPGNLFVAPLHEKNADGSTKFRLTFIDFGMVGRMPERLVEGLR
                     EAVISVGLQDATRLIKAYQTLGVLLPSANVKLLEEASAQVFDRFWGMSMNELRSIRHD
                     EMMKFGLQFRELMLTMPFQLPENLLLLGRTIGILSGMCTGLNADFNLWLQLAPYAKKL
                     TEGENGSAFDVFLNEAGELLKTLIAIPGRAERVLGRIERGELNMQMPLVNIQISYLER
                     SLNRLTGGVVFLGLLVSGAILYDGHALLAQGMFGGAVLALFYTTFLARGKRPFQ"
     gene            complement(4196..4411)
                     /locus_tag="PL000_13615"
     CDS             complement(4196..4411)
                     /locus_tag="PL000_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89988.1"
                     /translation="MNEEKRKSMRDKLPPEALEHMKAARQEMRKSMETILPPGFLEHR
                     RAARKEALLAFRSLIDAAIKRTEEKSS"
     gene            4511..5314
                     /locus_tag="PL000_13620"
     CDS             4511..5314
                     /locus_tag="PL000_13620"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC89989.1"
                     /translation="MRNSFTLSIKLIFTLAAIFVLTACGVIAPASPTPDVLATVVAQT
                     LTSMPNPEVAASTSTPVAATFTPIPSTFTPIPVSVAATSTPPIGGANFFFTSAENVNL
                     RVNPGRLFQVSRVLAKGTRLQVLGMAPGGQWFNVVNDEGIIGWVGKDFLEGNPNQQNL
                     LVITPTDVLTITGQVLDVNGVPVSGIGFAAVQGDKRDDGVTDAAGMFYIFLPSKFTGA
                     WTTSFVSVACPSTTMDANCNCLNGTCGKPDPEVVTGMLPLSAPLSYVWK"
     gene            complement(5387..7093)
                     /locus_tag="PL000_13625"
     CDS             complement(5387..7093)
                     /locus_tag="PL000_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNC89990.1"
                     /translation="MSSEEVVSRIPAFIREAVAEDLKSGRFKHIRTRLPPEPNGYLHI
                     GHIKAFLIDYFTAKEFGGELYLRFDDTNPSKEETEFVNAIEDDAKWLGIEWAKVTFAS
                     DYFDLLYEWAVRLIKMDLAYVDDQTQEEMSAGRGTLPKGTKWSETESAIRPGVDSPYR
                     NRPIEESLDLLERMKNGEFPEGSRVLRAKIDMTHPNILLRDPVMYRIMHVHHHRTGDK
                     WHIYPMYDWSHGQNDSMEGVTHSLCSNEYIIHRPLYEWFLEKLEAFPSRQIEFSRLNL
                     TYAMMSKRKLRKLVEAGIVRGWDDPRLTTLRGLRRRGVTPEAIINFVTGIGETKNDSW
                     VEMAQFEAVIRDDLNKRAIRRMAVLNPIKLVIDNYPEGVSEELDAVNNPEDPNAGTRK
                     VKFSKTIYIEQDDFRETPPPKYYRLYPGNEVRLRYAYFVKCTHVVKNDQGEVVEVHAT
                     YDPATHGGDSPDGRKVKSTIHWVSADHAIPAQVRMYDQLFTVERPDDGELESIINPKS
                     LDVIENAFVEPALAEVKTGENIQFERVGYFCADPDSTPEKPVFNLTVNLKDTWAKLEK
                     KK"
     gene            7255..7590
                     /locus_tag="PL000_13630"
     CDS             7255..7590
                     /locus_tag="PL000_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC89991.1"
                     /translation="MTESNQNRTNFAGTYFDRDAVLRLTRLLKIASWIVAVAYIFQFI
                     TNISVLILQISRDLIYIGGPTDLMLQIVWQIQPSLAGVIFYVALQAIAQTLLILLDIE
                     DNTRRAARK"
     gene            7711..7781
                     /locus_tag="PL000_13635"
     tRNA            7711..7781
                     /locus_tag="PL000_13635"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7743..7745,aa:Gln,seq:ctg)
     gene            7935..8405
                     /locus_tag="PL000_13640"
     CDS             7935..8405
                     /locus_tag="PL000_13640"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC89992.1"
                     /translation="MIVKAVEADGEKIQDITARAGVFNEEEVDCVREIWDEYITLGPE
                     QCGYQFIVYRDGEQVLGFACHGPRDLTSGVFDLYWIAVDPSTRRNGVGRALLTASEEA
                     VRKAGGRMLIAETSGTPHYESTRNFYFGMGYKDEARIKDFYTVGDDLVIFIKRI"
     gene            complement(8503..9522)
                     /locus_tag="PL000_13645"
     CDS             complement(8503..9522)
                     /locus_tag="PL000_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89993.1"
                     /translation="MHNERPKILIIDDDAVARAAVEGLLASEPYETYFASNGFDGISM
                     AVNLHPDVVLLDVMMPKMNGFEVCRSIRSMPEIAEAPIILITSLDDHESRIQGLRAGA
                     DDFVTKPYNSMELFARLQTILRLNRYRRIAEQKEELQTLNKQLLVAYNKTIEGWSQAL
                     DLRDRETEGHTQRVMNLTIRFAKAVGFSEADLEHVRRGSLLHDVGKLGIPDAILHKAA
                     KLTDEEWFIMRKHPVYAYQWLSSIDFLEPALDIPYCHHEKWDGSGYPRGIKGEAIPIS
                     ARLFAIVDVWDALCSDRPYRSAMSKADALDYIRSESGTHFDPELVKIFTELVKDEIEG
                     NSQVP"
     gene            complement(9557..10204)
                     /locus_tag="PL000_13650"
     CDS             complement(9557..10204)
                     /locus_tag="PL000_13650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC89994.1"
                     /translation="MAHIRIVLADDHKLVRQGVRSLLEAQPGFEVIGEASDGQEAYKL
                     IESLSPDIAFLDVMMPNLNGIETAKLVHQRALKTRIIFLSMHANATYAVRALQSGALG
                     YVLKDSDFTEVLNAVEHVLEGRRYLSTAIADEVLEMLLSAEAGKDESLDLLSPREREI
                     LQLVAEGNSNATIAEKLSLSVRTVESHRLNLTKKLRITSHADLVKFAINQGLITS"
     gene            complement(10224..11300)
                     /locus_tag="PL000_13655"
     CDS             complement(10224..11300)
                     /locus_tag="PL000_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC89995.1"
                     /translation="MNKQPYTILVIDDEEIARTTLAALLEKSNFQVEMAEDGIQGLEL
                     AKRIHPDVILLDVMMPKMNGYEVCKYIRSDEKIAEVPIIMITALDDHEAKLNGLSVGA
                     DDFLSKPFDRLELEIRLQTLKRVNRYRHLMEEREKLQEALNELSKKHEQLRILSRQIL
                     EAQENERRHVAVELHDEVGQLVTGLKLILERKESDMSMVVAEARSVTADLFQRLREMS
                     LNLRPTTLDDFGLFAALDGLFKRFTKQTMITINHNINPLEERRFDKVIETTVFRVVQE
                     ALTNIARHAEVNTANITITVTTNHLQVSIADAGKGFDLKSKDQSASIGLSGMAERVNM
                     AGGFFTLQSTPGKGTFILAEFNLN"
     gene            complement(11297..>13093)
                     /locus_tag="PL000_13660"
     CDS             complement(11297..>13093)
                     /locus_tag="PL000_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC89996.1"
                     /translation="HQHMVEMEMLYESGLALSQLLSPEEIGQKIIELLERKMDWHHTA
                     IRLYNPENDTLKLLAFSQPNTTNKDEHVAVKHQFNNKITKSSDGVSGWAVKNTQVVRH
                     GDISNDPRYIETYHGINSGLYVPVRLGDNVLGVISIESEKPNAFSEKDERLVVTLANQ
                     AAVAFENARLFQTAQQEIIERKKVEQLLAEERNQLAQHVQERTADLSNANMDLARAMR
                     VKDEFLASMSHELRTPLTGILGFSEALQLNAYGELSEKQLKPIKAIEDSGRHLLSLIN
                     DILDLSKIEAGKLELQFATCSITDVCTASLQIVKGMAQHKRQTMHYSPTSQPIAIFVD
                     ARRIKQVLVNLLSNAVKFTPEGGELGLDVEVSAKEQVARLAIWDKGIGIKPEDLPKLF
                     KPFTQIDSSLAREYSGTGLGLSLAQRLIELHNGKITVESSPGQGSRFTVTIPWSEQND
                     AQLEQTTDQKNSLFSQVKSEDSSPAVIMIADDNVAILNLLEDFLTPRNYKIAKAHSGL
                     ELLERITESHPDLLLMDIQMPGMDGLETIRHIRSHANPKIATTPIIAITALAMPGDRE
                     RCLQAGADEYMSKPLKLYELSIIIKKYIRKSK"
     assembly_gap    13094..13193
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13194..15616)
                     /locus_tag="PL000_13665"
     CDS             complement(<13194..15616)
                     /locus_tag="PL000_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89997.1"
                     /translation="MDEDIHILIVEDILDDANRVMQEVRKSIPGCQFEHAINEETFQS
                     ICNRFSPDLVFLDHQVTGFTTLEAFKSVKKSWPLTPCIVYARSFDEENAVEYIKAGAA
                     NYVTQNNAKRLGLAALKALEEKKELIEQSRVEQAMLESEEKFRSLIEQSSDGIVMIDE
                     HGMIIEWNSAESAITGIPRERAMGAPFWEIQYEILTPERRAQISPEEFKSHYFEVVRS
                     GNPELINKKFEIDFMTAQGEIRVLHQSSFIVTSQNGSRIGAIIRDVTEKKHAEERLQR
                     DRQILHEAQVMAKLGSWSVDLRTGEFNIDSECSTIAGCPPGLRDLSEILSLVHPEDLE
                     NARSAWQMLQQEGAPADLQLRITSNGKIKWLRIKAKLSAAKNDIPMSAIGVIQDITDT
                     KTAEQILHKSEIRLATVFRVSPISMAITRMKDNCLMDVNEAWEEITGYRKEEVIGRSV
                     LEINSWADAAERQQLVTLLRNRRNVQNFEFKLRHRSGEIRQLLMSAELLDLAGEECMI
                     TLALDITERKKTEYALQASEERFHKAFYSGPVGLAITRASNSTYIDANEAFSNIVGFS
                     REELLNQTSLGLGITTAKQKDEYVKLIREQGYIRDKEMELRHSSGEYRTVLGSMEVIE
                     LNHEACILSTAIDITERKKANKHLKESEENYRTLFQNAQVGMFRSRLDGSEILAANPK
                     FCEIFKYSEKELLSMPSLPLWAYPDTRPQKIEELLRKKVIRDYEIEIRTKDGEIRTCL
                     LSAQYFPEKEYLEGSIMDITSRKQAEDWISRLNHQLESILNSAGEGIYGTDSQGRVTF
                     INPTMARMIG"
     assembly_gap    15800..15899
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15900..>18046)
                     /locus_tag="PL000_13670"
     CDS             complement(<15900..>18046)
                     /locus_tag="PL000_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89998.1"
                     /translation="KQINGEPTEAEYRIKWPDDSIHWIWDRSFPVYDEEGKLTRTAGI
                     ATDITLNKKAEEELKERESFLQSIVGGSPGYIVSHDIEGHFLYANRRWPEQSEEEFRS
                     MKLQNMLHPNSLEPVMEAFRKAFVTRETQRVEQSVLTPNGDYHWFDVIYSPQVVNDEV
                     KSVTAFAYDINDRKLAEIALRESEEKYRGLMESLDNIIATMDYDGRFFYVNDKAADAL
                     GNKANEIIGKTVFDLFPDEVSASLMENIRIAIQEDRGVLMESKNLVRGEFRWYRTMIQ
                     PIHDAQGKVLHVLTNSTDIHDLKMAQHDLQELNNTLEERVQIRTAEVQDLYDNAPNGY
                     HSLDADGALVMINQTELNWLGYTRHEVLGKMKFRDLLGSENSSMFDGIFSQFVTSGKI
                     ADQTLSLIRKDGSQLPVLLNAYAIYDEYGNFTTSRCTITDITERVKIENALRESRDQL
                     SAANVSLEKAARLKDEFLASMSHELRTPLTGVLGLSEALQLNAYGELNEKQLRTVKAI
                     EESGRHLLELINDILDLSKIEAGKLDMQFTSFSINDICQASLQLIKGMANQKRQNVHF
                     TPPAKPIMLNADPRRIKQILVNLLSNAVKFTPEHGELGLVVEVNPADENISMAVWDKG
                     IGIKTENIQKLFKPFTQIDSSLAREYSGTGLGLSLAHRLVELHGGRIELESVFGEGSR
                     FTVILPWSDEEALSVYGELDAPSQSQSNKTGET"
     assembly_gap    18047..18146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18147..>20691)
                     /locus_tag="PL000_13675"
     CDS             complement(<18147..>20691)
                     /locus_tag="PL000_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC89999.1"
                     /translation="EQQSASIDHRIILPDGRVRHVTEKTQLIKDKNGNITRIIGTVQD
                     VTEQKLAQEALLKSQALLAEAQKIGHIGHWEWTSPDNKLICSDEIYNIFDMQKGDQIS
                     QQIITSIIPAEEVGRLRELEHATFSTHSDADYEYRINLSGGRRRWIHQKAKIVYSDRG
                     QPIRMMGTIQDITEYKQAQEDLQSAREFLQGVQDALSAHLAILDENGVILQVNSAWRK
                     FGKENGLDRPDACIGMNYLQVCDSAEGADASEAKQVAQEIRAVCSGEKTAAWVEYPCH
                     GLEEKRWYVVHITKFMDRGQVRIVLAHENVTERKMADEALRASEERYRLITSYVDEIV
                     WQVNTDLRFVYASPAATRVLGYTNEEAVNLHIYDLMDEEDAARMQGNIMARINREPGV
                     SNKPTEYRMRHKNGNLIETEALAAPIFDNANRLIGFAGITRDITERKVIERQLQEAER
                     KYRTLVEYSPVVVYVDEIGGEWQYISPQIKKILGYSPTDFLSDAQAFKKLIHPDDLHR
                     FQIQVNESLKKGQSLKLEYRMLTRDGSYVWIHDESDLTQSGDNGKYYLQGVFYDITER
                     KQAEEALAYNERRFRALIENIADGIQLVDMKGEVIYVSPSAARLLGYQQGEMEAPITS
                     LIHPDDLNQVAASISKSMTTQEALMQSYRVKHKDGTWRWFEGSITNRLTDPAIGSIVM
                     TFRDVTERKRSEDALRESEENFHSLFEDSPIAIWLEDFSQLKKYLDRLREQGIPNIKN
                     YLIEHPAELERLSGMVNILEINKTSARILGAQTKAEVLKSLPDYFTKESLKVFADEIA
                     ALADGQTQFKSEIPVKDLYGKEVIFDLTLTVQPGSEEDLSRVLVSFLDVT"
BASE COUNT         4582 a         4912 c         5147 g         5750 t
ORIGIN      
        1 ttcatttctg cgaaaatggg cacgtcggca tagtaaaacc cgtagcttgc cactacacgt
       61 ccgatccatg tggcggcgag gaatgcgaca atggcccaaa tgccaaaggt caattgtgct
      121 tcgcctgtga ccatgtcgaa gaaagccttg atgatgagcg cgggcgccac ctgccagcag
      181 aagcggatca atgccacgct gaacaggtcc acaacccaaa gccacgggcg gaagcggacc
      241 atttcgaaga tgactttcca cgctgggagc gcgggtactt tttgagtgaa ggttgtttcg
      301 gtcataataa aattccttgc ggtctttggc cgcaacgctg ggcatggacg ataaacggtt
      361 tgccgagtaa cgatctgctt tgtcaacggc tggctatgcc agcacttctt ccatgcctgt
      421 ttggagcaat tgataaaaac gcgagttcaa gtcggcggcc aattcttttc gattgccgta
      481 ttcgctgact ttgcctttgt ccaggatcat gacattgtcg gcacgttcga tcgtgtccag
      541 ccgatgtgcg atgatgatgg ctgtgcggtt tttcaatagt ttgtcgatgg cgcgttccaa
      601 cttctgttct gtggcagggt caaggcgcga tgatgcttcg tcgaggatca ccaggcctgg
      661 atttcgtaaa aagacccgtg tgaaagccag caattgcgct tcacccgcag acagggaacg
      721 ggaacctgtt tcgagttcag tatccagccc tttgggcagg ttctgatacc aatcgccgag
      781 ttccaattcc tcgagagtcg caatgatctt ttcatcggat atcgtgcggt caaaaaaggt
      841 caggttatca cgaatgctgg cacgaaaaag ctgaacatcc tgagtgacga tggcgatgtt
      901 gcgccgcagg gtttcgatgt gtgcatcgcg caagtctgcg ccgttgacct tgatgctgcc
      961 tgcgtttaca tcgtacagac ggaagatcaa acgtccaagg gtggttttgc cactgcctgt
     1021 gcggccgagc agcccaagta cggaaccagc tctcaggtcg aaggaaagac cgttgatgac
     1081 tgtatcctcg ccgttgtagg agaaggtcac atcttcgaag gtcaattcaa gcggacgtgc
     1141 tggaatctcc acgccgaccc cgctgacaac ctccgctttg aaattgcgga attcggtcag
     1201 gcgttccacg caggcgccga tggtctgaaa actttcgatc tcgtgggtca tcgcccagaa
     1261 tggttcttcc agcagattga tgtagtgtac gaagagatac accgtgccga tggtgaccag
     1321 acccgcagag aacagccaat atccgctgga gatggcgagc aggtttccag tggtcaatgc
     1381 aaagcccatg gcgttttcga tgatccagcg tttaaagtgt gccttgcggt cgtgggtcaa
     1441 cttgatgccc tgcaaacgga acaattctcg aatggaataa tccacggcgc cgctggagcg
     1501 gatatcttct gtgcctgcca gttgctcctc gataaaaccg aagatctgtg catccgcttc
     1561 tcgcctggcc ttttgatgcg gcacggcgat gtctttgagc cttccgagaa caaagatcgt
     1621 gaggattgaa taaacggcga atgccagccc ggcgcgccag tcctcaagga aaagcgccac
     1681 gaggattcca acaagcagca agccattggc gatcaaattc agcgcgaact gtgaaaagaa
     1741 ggttgccagc tcggtcacgt caccgtcaat gcgctcgatc aattcgccgg gcgtgtggtc
     1801 gttgtggaat ttcatatcaa ggtgcagggc gtgctccgcc agttcggcgc gcaggtcgtt
     1861 ggtggcggtc caggctacat tctcgccgag gtaggtcacg cttacagcca cggcctgttg
     1921 gatcaaggca atgccaatga atgcaatcgc ggtccacatc agcgtggata gggcttcgcc
     1981 agccagcgcc gagtcgataa agcggcgcat gatctgcggg gcaaagattc gcaagccgat
     2041 actgccaaac aataaagcgg cgagcagaat gaaacgtccc ttttgtgggc ggatgtggtc
     2101 ggagagtaga ttccagtaag atttgaatga taggttcatg atgttttgtc cattttgaca
     2161 ataaaaaaca cggcgcaggt gcgccgtgtt tggaaagaca cagaaagttg aatccgtggt
     2221 taacggtcaa taggcgcact tcgagatgat tgaattattc ggtgtagttt ggctgtaaga
     2281 ggcaaattca tttgaagtgc gttcctttcc tggagtttag tgtttgcagt gtacaacact
     2341 tgcgcgttgc ttgtcaagga aattacattg cgcaattcaa tcacaggcat tgatcaggtt
     2401 tgcaaaacca ccaaacgggc tacaattgaa gcccatggag gctttatgaa aaacacatat
     2461 cggatgcgct attggcgtat cgttctgttc tttgcgcgtg tcaccctgag tattattttc
     2521 tgggatatct ttctgccgcg cattggcttg cgctttcttt caagaaatac gcgcccgcag
     2581 cggctgcgtg gcattgccgt ccgttttcgc gccctggcga ttcgacttgg cggcgtgatg
     2641 atcaaggtcg ggcaatttct ctcggcgcgg ctggatgttt taccccctga gatcaccgac
     2701 gaattggcag gattgcagga tgaagtgccg cctgtggatt atgaagcgat ccgcgtccaa
     2761 accgaactgg agcttggttc tgccctcgaa aagatatttc tcacgtttga aaaagccccc
     2821 attgccgccg cttcgttggg acaagtccat cgcgcccgat tgttcccgca tgaagctgag
     2881 acaattggtt ttgaaaatat cgtcgtgaag gtgttgcgcc ccaatatcga gcagatcgtg
     2941 gatgtggata tgtctgctct acgggtggtg ggaggctggt tgaagcgtta tcggcctgtg
     3001 agtgaccgcg ccgatgtgcc tgccattgtg gaggaatttg ctgccaccat ccgcgaagaa
     3061 gtggattatc tttcagaggg gaaaaatgcc gaggcttttg cgatcaattt caagggcgat
     3121 gagaacgttt atgtcccgcg tgtagtgtgg acccgttcca gtcgtcgtgt gcttgcgttg
     3181 gaggatgtat ctgcgatcaa gatcactgat tatgaagcca ttactgccgc agggatcagc
     3241 cgtgccgcag ttgcagaccg cctgcttgcc acatatttga agcagatctt tgaagatggc
     3301 tttttccatg ccgatccgca ccctgggaat ttgtttgtcg cgccgctgca cgaaaaaaat
     3361 gcagatggct caacgaaatt ccgcctgaca tttattgatt ttggcatggt cggtcggatg
     3421 cctgagcggt tggtggaggg gttgcgggag gctgtcatct cggtcggcct gcaagatgcc
     3481 acccgtttga tcaaggcgta tcagacattg ggcgtgctgc ttccaagtgc gaatgtgaag
     3541 ttgttggagg aggccagtgc ccaggtcttt gaccgttttt ggggtatgag catgaacgaa
     3601 ctgcgcagca tccgtcacga tgagatgatg aagtttggtc tgcaattccg cgagttgatg
     3661 ctgacaatgc cgtttcagtt gccagaaaat cttttgctgc tgggcagaac gatcggaatt
     3721 ttatcgggca tgtgtacggg gttgaacgcc gatttcaacc tctggctgca acttgcacct
     3781 tacgccaaga agttgacaga aggcgaaaat ggctccgctt ttgatgtgtt tctgaatgaa
     3841 gcaggcgaac tgctcaagac tttgatcgcc atccctggca gggcagagcg tgtgctgggg
     3901 cgcattgaac gcggtgagtt aaacatgcaa atgccgctgg tcaatattca gatcagctac
     3961 ctggaacgtt cgttaaaccg attgacgggg ggagttgttt ttctcgggct gttggtttcg
     4021 ggcgcgattt tatatgatgg gcacgctttg ctggcacagg gaatgtttgg cggggcggtt
     4081 ctggctttgt tttatacaac ttttcttgcc agaggcaagc gcccatttca atgatcggtg
     4141 ctttgcatat ttcaacttac aaaaaaactc ccagcccttt ggctgggagt tttgcttatg
     4201 aagatttttc ttctgttctc ttgatcgcgg catcaatcag gctgcggaat gccagaagtg
     4261 cttccttgcg ggcggcacgg cggtgctcaa ggaagccagg tggaaggatg gtttccatgc
     4321 tttttcgcat ttcctggcgt gcagctttca tgtgttccaa cgcctcaggc ggaagtttat
     4381 ctctcatgga tttgcgtttt tcttcgttca taggtcactc cttttgtctt ttctgattat
     4441 agcaccgttg gtactgggtg ggtgttttcc acctgctaat cgcttacaat gggcgaaaaa
     4501 agaggagcca atgagaaatt cgttcacact ttcgatcaag ttgatattta ccctggcggc
     4561 tattttcgtg ttgaccgcat gtggtgtcat cgcacccgca tcaccaacac ccgatgtgct
     4621 cgccaccgtt gtggcccaaa cgctgacttc catgccgaat cccgaagtag cagcatccac
     4681 ctccacacct gttgccgcta catttacccc gatcccatcc acgtttaccc cgattccagt
     4741 ttcggttgca gctacgtcaa cgccgccgat cgggggggcg aatttctttt ttaccagtgc
     4801 cgagaacgtc aatctgcggg taaacccggg caggctgttt caggtcagcc gtgtgttggc
     4861 aaaaggaaca aggttgcaag ttttgggtat ggccccaggc ggacagtggt ttaacgtcgt
     4921 aaatgacgaa ggcatcatcg gctgggtcgg aaaggatttt ctggaaggca accccaacca
     4981 gcaaaacctg cttgtaatta ccccaactga tgttctgacg ataacagggc aggtcctgga
     5041 tgtgaatgga gtccctgtca gcggaattgg gtttgccgct gttcaaggtg acaaacggga
     5101 tgatggcgtc acagacgcag cagggatgtt ttacatcttc ctgccctcca agtttacagg
     5161 cgcgtggaca acaagctttg tctcggtagc ctgtccaagc accaccatgg atgccaattg
     5221 caattgtctc aatgggacat gcggaaagcc cgaccccgag gttgttacag ggatgttgcc
     5281 gctttctgca ccattgagct atgtctggaa gtaaacggag taacataaac aaaaaacgct
     5341 gccaattgtg gcagcgtttt ttgttttgtg accaaccaaa taaaggttat ttcttctttt
     5401 cgagcttcgc ccaggtatct ttcaaattga cggtcaaatt gaagacgggt ttttcaggcg
     5461 tagagtcggg gtcggcgcag aagtatccca cgcgctcgaa ttgaatattt tcgccagttt
     5521 ttacctctgc gagtgcgggt tctacaaagg cgttttcaat cacatccagt gatttggggt
     5581 tgatgatgct ttccaattca ccatcgtcag gcctttccac ggtgaataac tggtcgtaca
     5641 tacgcacttg ggcaggaatg gcatgatctg cagaaaccca atggatcgtg gatttaacct
     5701 tgcggccatc cggtgagtcg ccgccgtgtg tggcagggtc atacgtggcg tgaacttcca
     5761 ccacttctcc ctgatcgttc ttcactacat gcgtgcattt gacaaaatag gcgtatcgca
     5821 ggcgcacttc gttgccagga tacaggcggt aatacttcgg cggcggagtc tctcggaaat
     5881 catcctgctc aatataaatc gtcttcgaaa atttcacctt gcgggtgccg gcgttcggat
     5941 cttcggggtt gttgaccgca tctaattcct ctgatacgcc ttcggggtaa ttgtcgatca
     6001 ccaatttaat cggattcaaa acagccattc ggcggatcgc gcgtttgttc agatcgtcgc
     6061 ggatgacggc ctcgaattgc gccatctcca cccagctgtc attcttggtc tcgccgatgc
     6121 cggttacaaa attgatgatg gcttcaggcg tcacgccccg gcggcgcaat ccacgcaggg
     6181 tggtcagacg cggatcgtcc cagccgcgga caatgcctgc ctccacaagt ttgcggagct
     6241 tgcgcttgct catcatggca taggtcaagt tcaggcgcga aaactcgatt tgcctgcttg
     6301 ggaaggcttc caatttttca agaaaccatt cgtacaatgg gcgatggata atatactcat
     6361 ttgaacacag tgagtgtgtc acgccttcca tcgagtcgtt ctgtccatgc gaccaatcgt
     6421 acatcgggta aatgtgccat ttgtcgcctg tgcggtggtg atggacgtgc ataatgcggt
     6481 acatcacagg gtcgcgcaac aaaatgttgg ggtgcgtcat atcgattttt gcacgcagta
     6541 cacggctgcc ttcagggaat tcaccgttct tcatgcgttc caacaagtcg aggctttcct
     6601 cgatcggtcg gtttcgataa ggagaatcaa ccccagggcg aatcgcagac tcggtttcac
     6661 tccactttgt ccctttgggc aatgtacctc gtccggcact catctcttcc tgagtctgat
     6721 cgtcaacgta tgccagatcc attttgatca ggcgcaccgc ccattcgtaa agcaggtcaa
     6781 agtaatctga tgcaaaggtc actttagccc attcaatgcc aagccacttc gcatcgtctt
     6841 cgatggcatt tacgaattca gtttcttctt ttgagggatt cgtgtcatcg aaacgcaaat
     6901 ataattcgcc gccaaattcc tttgccgtga aataatcgat caagaacgcc ttgatgtgcc
     6961 caatgtgcaa gtatccattc ggctcgggag gcaggcgtgt gcggatatgt ttgaaacgcc
     7021 cactttttag atcctcagca actgcctcgc ggatgaacgc ggggatacga cttacgactt
     7081 cttctgaact catggatggc tcaaccttcg ctgtgatttt atgcggatga aaaccgcttc
     7141 tttcataacc tcaacctgat cttgcttcag gaacattttc ccacccgaag ccgtcacagg
     7201 cgaaaagcac gccaattata actgctttat aataacccta ccctggagga acccatgact
     7261 gaatcaaacc aaaatcggac caattttgca ggcacatact ttgatcgtga tgccgttctg
     7321 cggttgactc gtttgttgaa gatcgcttcc tggatcgtgg ctgtcgccta catctttcaa
     7381 ttcatcacca acatctcagt gttgatcctg cagatatcac gagacttgat ctatattggc
     7441 ggcccaaccg atctgatgct gcaaattgtc tggcagatac agcccagcct tgctggggtg
     7501 attttttatg ttgccctgca agccattgct cagaccttgt tgatcctgct tgatattgaa
     7561 gacaacacgc gccgcgccgc ccgcaagtaa accataaaaa ataaaccttg cttcatcctg
     7621 atgggcgtga taaaataccg cccgctggaa gaatagtggg gcttgtctaa aagcagaaca
     7681 ggtaaccctg aatcagctgt ttaggcattt tggggagtgg tgaaatggca tcacgttgcg
     7741 ctctggacgc aaaatttgag gttcaaatcc ttgctcccca gcttgaaagc aattgctcaa
     7801 aaggcaattg cttttttttg tatccctgcc gtctgatttg cgtataggca ttttgagtta
     7861 tataggatgg atataattac gtctgatcaa aatccaagtt ccaaacttgt tttggaacaa
     7921 aataaggaaa agtcatgatt gtcaaagctg tggaagcaga tggtgaaaag attcaggata
     7981 ttactgcaag ggcgggtgtt ttcaatgagg aagaagtgga ttgtgttcga gaaatttggg
     8041 atgaatacat tactcttggc ccggagcaat gtggatatca gtttattgtt taccgagatg
     8101 gggagcaggt tcttggcttt gcctgtcatg gcccacgaga cctgacaagc ggtgttttcg
     8161 atctttactg gatcgcggtc gatccttcca cacgtcgaaa cggagtggga cgtgccttgt
     8221 tgaccgccag cgaggaagcg gtccgcaaag caggtgggcg aatgttaatc gctgaaactt
     8281 ctggaacacc acattatgag tcgactcgta atttctattt tggcatgggg tataaagacg
     8341 aggcaagaat caaggatttt tatacggttg gcgatgatct agtgatcttt atcaaacgca
     8401 tttaaataaa tatccaccat ttgcactgca gttaatttga cagtagcgta aatggtggat
     8461 ataaaattgg cgtacttgtc tttttgaaaa accctgaaaa aattaaggca cctggctgtt
     8521 tccttcgatt tcgtccttca caagttctgt gaagatcttt acgagttcgg ggtcgaaatg
     8581 tgtgccagat tcactgcgaa tatagtcaag cgcatcagct ttggacattg cgcttcgata
     8641 ggggcgatct gagcataaag catcccatac atctacgatt gcaaaaagac gcgctgaaat
     8701 tggaatcgcc tcgcctttga tcccgcgtgg atatccgctt ccgtcccatt tttcgtgatg
     8761 acaataggga atatccagag cgggttcgag gaaatcaatg gatgaaagcc attgataggc
     8821 atagactggg tgctttcgca tgataaacca ttcttcatcg gtaagtttgg ctgctttgtg
     8881 gaggatggca tctggaatgc ccaacttgcc tacatcgtgg agcagagagc ctctacgaac
     8941 gtgttcaagg tcagcttcag aaaacccaac tgccttggcg aaccgaatgg tcaggttcat
     9001 cacacgttgt gtatgacctt ctgtctccct gtcgcgcagg tccagcgctt gcgaccagcc
     9061 ttcaatggtc ttgttgtaag caaccaggag ttgtttgttc agggtctgta attcttcctt
     9121 ctgttctgca atgcggcggt agcgattgag tcgaaggatg gtttgcaggc gggcaaataa
     9181 ttccatgctg ttgtatggct ttgtgacaaa atcatccgcc ccggccctca gcccttggat
     9241 gcgcgattcg tgatcatcca gcgacgtgat caaaatgatg ggggcctcgg cgatctccgg
     9301 catggaccga atgctcctac acacctcaaa accattcatt ttaggcatca tcacatccaa
     9361 taggaccaca tctggatgca ggttgactgc catggatatc ccgtcaaagc catttgaagc
     9421 gaaataagtc tcgtatggtt cacttgccag caggccttcg actgcagcgc gggcaacagc
     9481 atcgtcatca atgatcaaga tctttgggcg ctcattgtgc attttctttc tcctttccat
     9541 gcgttagtac aaacaatcag gatgtgatca acccttgatt gatggcgaat ttcaccagat
     9601 cggcatgtga agtgatgcgc agcttttttg tcagattgag ccgatgcgat tcgactgttc
     9661 gtacgctaag tgacaacttt tccgcaatgg tggcgttgga atttccttca gcaaccaatt
     9721 gcagaatttc ccgttcacgg ggggatagca gatcaagtga ttcatccttg cctgcttcgg
     9781 cactcaaaag catttccaat acttcgtctg caatggcagt gctaaggtat cttctgcctt
     9841 ccaatacatg ttcaacagcg tttaaaacct cggtgaagtc agaatctttc aagacatatc
     9901 cgagtgctcc actttgtaat gcgcggacgg catacgtggc atttgcgtgc atcgataaaa
     9961 agatgattcg tgtctttagc gcccgctgat gcacaagttt ggcagtttca atgccattca
    10021 ggttgggcat catcacatcc agaaacgcga tatccggtga caagctttct attaatttgt
    10081 aagcttcctg tccgtcgctg gcttctccaa ttacttcaaa acctggctgg gcctcaagca
    10141 gggatcgaac cccctggcga accagtttgt gatcgtctgc aagaacaatt cttatgtgcg
    10201 ccatgttttt cgctcctttc cattcagttc aggttgaatt cggcaaggat aaatgtccct
    10261 ttgccgggag tggattgcag ggtaaagaat ccgcctgcca tgttcacgcg ttccgccatg
    10321 cccgaaagcc caatggaggc gctttgatcc ttgcttttga ggtcgaatcc ctttcctgca
    10381 tcggcgatgc tgacctgtaa atggttggtt gtgacagtga ttgtgatgtt ggctgtgttc
    10441 acttcagcat ggcgggcaat atttgtaagg gcttcctgaa ccacacggaa aacggtggtt
    10501 tcaatgactt tatcaaaacg gcgttcctcc agtgggttga tgttatggtt gatcgtgatc
    10561 atggtctgtt ttgtaaatcg tttgaacagg ccgtccaaag ccgcgaaaag gccgaaatca
    10621 tccagagtgg tgggacgcag gtttaatgac atttcacgaa gcctttgaaa cagatcggca
    10681 gtgacagaac gggcttcggc aacaaccatg gacatgtcgc tttcttttcg ttcaaggatc
    10741 aactttaatc ctgtaaccag ttgtccgacc tcgtcgtgca actcaaccgc cacatggcgg
    10801 cgttcatttt cctgcgcttc caatatctgg cgcgatagta tgcgtaactg ctcgtgcttt
    10861 ttggagagtt cattaagggc ttcctgaagt ttttcacgtt cctccatgag gtgacgatag
    10921 cggtttacgc gtttgagtgt ttgcaggcgg atctccagtt cgagcctgtc aaatggcttg
    10981 gagagaaaat cgtcagcacc gacactaagc ccgttcaatt ttgcctcgtg gtcatcaaga
    11041 gccgtgatca tgatgatggg tacttccgcg atcttttcat ctgaacgaat gtatttgcaa
    11101 acctcatacc cgttcatttt gggcatcatg acatccagca gaattacatc cgggtggatg
    11161 cgttttgcaa gttccaggcc ttgaatgcca tcttccgcca tttccacttg aaaattggat
    11221 ttttcaagca gggctgccaa ggttgtacgt gcaatttctt catcatcgat aacaaggatg
    11281 gtgtacggct gtttgttcat tttgacttcc ttatatattt ctttatgatg atggaaagtt
    11341 catatagctt tagcggcttg ctcatatatt cgtcggcgcc agcctgcagg caacgttccc
    11401 ggtcacctgg catggccagg gctgtgatcg cgataatggg ggtggtcgct attttggggt
    11461 tggcgtgaga acgaatatgt cggatggttt ctaatccatc catgcctggc atttggatat
    11521 ccatcaaaag caggtcagga tgggattctg taattctctc cagcaattct aaaccactgt
    11581 gtgccttggc gatcttgtag ttgcgggggg tgagaaagtc ttcaagcaga tttagaatcg
    11641 ccacattgtc atcagcgatc atgatgactg ccggtgagga atcttcagat ttaacttgcg
    11701 aaaacaagct atttttctgg tcagtcgttt gttcaagttg tgcatcgttt tgttccgacc
    11761 acggaattgt gactgtgaaa cggctgccct gccccggcga gctttctact gtgatttttc
    11821 cattatgtaa ctcgatgaga cgttgtgcaa gtgacaggcc caatccggtg cctgagtatt
    11881 cccgtgccag gctgctatcg atctgggtaa acggcttgaa taatttgggc aggtcttcag
    11941 gcttaatacc tatgcccttg tcccaaatgg cgagtcgggc aacctgctct tttgcagaga
    12001 cttccacatc caaccccaat tcgccgcctt ccggggtgaa tttcaccgca ttgctcagaa
    12061 ggtttaccag aacctgtttg atgcgccttg catcaacaaa aatcgcgatc ggctgactgg
    12121 tgggtgaata atgcatggtt tgacgtttgt gctgtgccat tcctttgacg atctgtaaac
    12181 tggcagtaca gacatcagtg atcgaacagg tggcgaattg tagttccagc ttgcctgcct
    12241 cgatcttgga aaggtcaaga atatcattga tgagcgatag cagatggcgt ccgctgtctt
    12301 caatcgcttt gatgggcttc aattgttttt cgcttagttc tccataggca tttagctgca
    12361 gggcttcaga gaatccgaga atacctgtaa ggggagtgcg tagttcgtgg ctcatactgg
    12421 caaggaactc atctttcaca cgcatggcac gtgccagatc catatttgca ttgcttagat
    12481 ctgctgttcg ctcttgaaca tgttgtgcca gttgattccg ttcctccgca aggagttgtt
    12541 ccaccttttt acgttcgatg atctcctgtt gggcggtttg gaacaaacgg gcattttcaa
    12601 atgcgacagc tgcctgattg gcaagagtaa cgacaaggcg ttcgtctttt tcgctgaagg
    12661 cattgggttt ttcgctttcg attgagatca caccaaggac attgtccccc agcctgacgg
    12721 gcacgtataa gcccgaattg atgccgtggt atgtctctat atatcgcgga tcattgctga
    12781 tatctccgtg acgtacaacc tgtgtatttt taactgccca gccgcttact ccatcacttg
    12841 attttgtgat cttgttatta aattggtgtt ttactgcgac gtgttcatcc ttgttagtgg
    12901 tattaggctg actgaaagcg agtaacttta gggtgtcatt ttctggattg tagaggcgaa
    12961 tggcagtatg gtgccagtcc atttttcgtt caagtaattc gatgatcttt tgcccgatct
    13021 cctccgggct aagcaactga ctgagtgcca gcccgctttc gtagagcatt tccatttcca
    13081 ccatgtgttg atgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncctatca
    13201 tccgtgccat ggttgggttg ataaaggtaa ccctgccttg tgagtctgta ccgtagatcc
    13261 cttcaccggc cgagttgaga atgctttcca gttggtggtt gaggcgggat atccaatctt
    13321 ctgcctgctt gcgagaggtg atatccataa tggagccttc cagatattct ttttctggaa
    13381 aatattgtgc agataagagg caggtacgta tttctccatc ctttgtgcgg atctcgattt
    13441 cataatcacg tatgaccttt ttgcgtaata gctcttcaat tttttgcggc ctggtatctg
    13501 ggtatgccca caatggcagg gagggcatgg aaagtaattc tttttcgctg tacttaaaga
    13561 tctcgcagaa ctttgggttg gccgcaagga tctctgaacc atccaggcgg gaacgaaaca
    13621 ttccaacctg tgcattttga aatagggtac ggtagttttc ctctgattcc ttcaggtgtt
    13681 tattcgcctt cttgcgttca gtgatgtcga ttgcggtgga aaggatgcag gcttcatggt
    13741 taagttcaat gacctccatg gatccgagaa ctgtgcgata ttctcctgaa ctatggcgta
    13801 attccatttc cttgtcacgg atataaccct gttccctgat caatttgacg tattcatctt
    13861 tttgtttggc ggtcgttatt cccagaccca gggaggtctg attgagcaac tcttcgcgac
    13921 taaaccccac gatgtttgag aaggcttcat tggcatcaat gtatgtgctg tttgatgcac
    13981 gggtgatggc aagccctacc ggccctgaat aaaaagcttt atgaaaccgt tcttcacttg
    14041 cctgcagggc atactcggtc ttctttcttt cagtgatatc gagcgccagt gtgatcatac
    14101 attcttcacc tgccagatcc agcaattccg ctgacattaa tagctgacga atctcacctg
    14161 accggtgacg gagcttgaac tcaaaattct gcacattgcg acgatttcgt aatagagtga
    14221 cgagttgttg cctctcagca gcatctgccc aactgttgat ctccaacacg gagcggccaa
    14281 tgacctcttc ttttcgataa ccggtgatct cttcccaggc ttcattcaca tccataaggc
    14341 aattgtcttt catgcgtgtg attgccatac tgatgggact gacacgaaat acggttgcaa
    14401 gccgaatctc gcttttgtgc aatatctgct ccgctgtttt ggtgtcagtg atatcctgta
    14461 taactccaat tgctgacatt ggaatgtcat ttttggcagc ggataatttt gctttaatgc
    14521 ggagccattt gattttcccg ttactggtga tacgcagttg aaggtcggcg ggcgcgcctt
    14581 cctgttgcag catttgccat gcggatcggg cattttcaag atcttcgggg tgaacgaggc
    14641 tgaggatctc ggaaagatca cgtaacccag gcggacagcc agcgatcgtg ctgcattccg
    14701 aatcaatgtt gaattcacct gttctcaaat cgacagacca gcttccaagt tttgccatca
    14761 cctgggcttc atgtaagatc tgcctgtctc tttgcagtct ttcttcggcg tgcttttttt
    14821 cagtgacatc acggatgatg gctccgatgc gactgccgtt ttgactcgtg acgataaaag
    14881 aactttgatg aagcaccctg atctcgccct gtgccgtcat gaagtcaatt tcaaattttt
    14941 tatttatgag ttctggattg ccacttctga ccacttcaaa atagtgagat ttgaattctt
    15001 caggtgaaat ttgagcgcgt cgttcgggcg ttaagatctc atattgaatt tcccaaaatg
    15061 gggcacccat ggcgcgctct cgagggattc cggtgatggc ggattcggcg gaattccatt
    15121 ctatgatcat gccatgttca tcgatcatga cgatcccgtc actggattgt tcgatcaggg
    15181 agcggaattt ttcttcactt tccaacatgg cttgctcaac tctactttgt tcgatcagct
    15241 ctttcttttc ctccaatgct ttgagagcag ccagacccag ccgcttggca ttgttttggg
    15301 tcacgtaatt ggctgcgcct gccttgatat attcaacggc attctcttca tcaaaggacc
    15361 ttgcatagac gatacatggt gtcaacggcc agcttttttt gacagacttg aaggcttcca
    15421 aggttgtaaa ccctgtcacc tgatgatcga ggaagaccaa gtctggcgag aaacggttgc
    15481 aaatgctttg aaaggtttct tcgtttatgg cgtgctcaaa ttgacatccc ggtatggatt
    15541 tgcggacctc ctgcatgacc cggttggcat catcaagtat gtcttcaacg attaagatat
    15601 ggatgtcttc gtccatggtg tttccttttc gggcacggtt ggttttattg cggacgtttt
    15661 cttttttgaa taagcatggc cagttcattt aatttcatag gcttgctcat ataatcggtt
    15721 gcgcctgcac gcaggcatcg ttcacggtcg cccggcatgg ccagggcggt gaccgcgatg
    15781 atcggaacag aagcaattgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    15901 tggtttcgcc cgttttattg gattgggatt ggctgggggc atccaattct ccgtacactg
    15961 acagcgcctc ttcatcggac catggcagga tgaccgtaaa acggctgcct tcaccgaaga
    16021 cgctttccaa ttcaattctg cctccatgca gttcaaccaa ccgatgggcg agggaaagcc
    16081 caagacctgt tccggagtat tcgcgtgcca ggctgctatc gatctgggta aagggtttga
    16141 aaagtttttg aatgttttcg gttttgatgc cgataccctt gtcccatact gccatgctga
    16201 tattttcgtc ggctgggttg acttcaacga ccagtcccag ttctccgtgc tcgggcgtga
    16261 atttcaccgc attgctgagc aggttgacca gaatttgctt aatgcggcgc gggtcggcgt
    16321 ttaacataat gggcttggcc ggtggcgtga agtgtacatt ctggcgtttt tgatttgcca
    16381 tgcctttgat cagctgcagg ctcgcttgac agatatcgtt gatggagaag gacgtaaatt
    16441 gcatgtccag ttttcctgct tcgatcttgg aaaggtcaag gatgtcattg atcaattcca
    16501 agagatgacg cccgctttct tcaatggctt ttacggttct taattgtttc tcgttcaatt
    16561 cgccgtatgc attaagctgc aaggcttcgg aaagccccag cacccctgtg agtggtgttc
    16621 gcagttcgtg gctcatactg gcaaggaact catctttaag gcgggcggct ttttcaagag
    16681 agacattggc tgcgcttaac tggtctctgc tttcgcgtaa tgcgttttcg atcttgactc
    16741 tttcagtgat gtctgtgatc gtgcagcggc tggtggtgaa gtttccgtat tcgtcataaa
    16801 tggcatatgc attgagtaat acgggcagct ggcttccatc tttgcggatg agactcaggg
    16861 tttggtctgc aatttttccg ctggttacga attgcgagaa tatcccgtcg aacatggagc
    16921 tgttttcact cccgagcagg tcgcggaact tcattttccc gagcacctca tggcgtgtgt
    16981 atcccagcca gttcaactct gtctggttga tcatgaccag cgcgccatct gcatccagtg
    17041 agtgatatcc atttggcgca ttgtcgtaaa gatcctgcac ctcggcggtt cggatctgca
    17101 cacgttcctc gagcgtgttg ttcagttcct gcaggtcgtg ctgggccatt ttcaagtcgt
    17161 ggatgtcggt ggaatttgtt agaacatgca gaacctttcc ctgtgcatca tggattggct
    17221 ggatcatggt gcggtaccag cgaaattcac cacgaaccag gttcttgctt tccatcaaca
    17281 cacccctgtc ttcttgaatg gcgatgcgga tattttccat aagcgaggca gaaacctcat
    17341 ctggaaatag atcgaacact gtctttccaa taatttcatt ggctttgtta ccaagggcat
    17401 cggcagcctt gtcatttacg tagaagaagc ggccgtcata atccatggta gcgatgatgt
    17461 tgtccaggct ttccatcaag ccgcggtatt tttcttcact ttcacgcagg gcaatttcag
    17521 ccaatttgcg gtcattgatg tcgtaagcaa aagcggtgac actttttact tcatcattca
    17581 caacctgcgg actgtagatc acatcaaacc agtgataatc gccattgggt gtcaatacag
    17641 attgttcaac ccgttgtgtt tcgcgggtta caaatgcctt tcggaaagcc tccatcacgg
    17701 gttcaaggct gttcggatgc agcatatttt gaagcttcat acttctaaac tcttcttcac
    17761 tttgctccgg ccagcgacgg ttggcgtaaa gaaaatggcc ttcaatgtcg tgagaaacga
    17821 tataacccgg cgaacctccc acaatggatt gcaggaagct ctcgcgttct ttgagctctt
    17881 cttccgcctt tttgttgagc gtgatgtcgg tcgcaattcc ggccgtgcgg gttaacttgc
    17941 cttcctcatc gtaaacgggg aagcttcgat cccagatcca atggatggaa tcatcaggcc
    18001 atttaatacg atattccgcc tcggtgggct cgccgttgat ctgtttnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnngtaa catcaaggaa tgagaccagt acacgtgaga
    18181 gatcttcctc gctgcctggc tgcactgtca gggtcaggtc aaaaatcacc tctttcccat
    18241 acaggtcttt gactggaatt tcactcttga attgcgtctg gccatcggca agtgcggcta
    18301 tttcatctgc aaatactttc aacgattcct tggtgaagta atctggtaat gatttgagaa
    18361 cctccgcctt ggtttgggcg ccaagaatcc tggcgctggt cttattgatc tccaggatat
    18421 ttaccatgcc ggataaccgc tcaagttcag cggggtgttc gattaagtag ttctttatgt
    18481 tggggatacc ctgttcgcgc agacggtcta aatatttttt gagctgcgag aaatcctcca
    18541 accagatcgc aatgggggaa tcttcgaaca aggaatggaa attttcctcg ctttcacgca
    18601 gtgcatcttc cgagcgcttt cgttcggtca catcacggaa cgtcatcacg attgagccaa
    18661 tggcagggtc ggtaaggcgg ttggtgatac tcccttcaaa ccagcgccag gtaccgtcct
    18721 tgtgtttcac acgataagac tgcatcaaag cctcttgtgt ggtcatggat ttggagatac
    18781 tggcagccac ctggtttagg tcatctggat ggatcaggct tgtaattggc gcttccatct
    18841 caccttgctg atatccaagc aggcgggctg cagatgggct gacatagata acctcgcctt
    18901 tcatatccac cagttgaatg ccatctgcaa tattttcgat cagcgcacgg aagcgtcttt
    18961 cattgtaggc caatgcttct tctgcctgtt tgcgttcggt aatatcgtaa aagactccct
    19021 gcaggtagta ttttccgttg tccccgcttt gggtcaggtc agattcgtca tgaatccaca
    19081 cataactccc atcacgggtc agcatgcggt attcaagttt gagcgattgt ccctttttca
    19141 ggctttcatt cacctgaatc tgaaaacgat gcaggtcatc aggatgaatt aacttcttaa
    19201 aagcctgggc atcactgagg aaatcggtgg gcgaataccc aaggatcttt ttgatctgcg
    19261 ggctgatgta ttgccactct ccgccaattt catcgacata aacgactaca ggagaatatt
    19321 caacaagggt gcgatatttt ctttccgctt cctgtaactg cctttcgatc acctttctct
    19381 cggtaatatc gcgggtaatg cctgcaaaac cgatcaggcg gtttgcgttg tcaaagatcg
    19441 gagcggccag cgcttcggtc tcgattaagt tcccattttt gtgccgcatt cgatattcgg
    19501 ttggcttgtt gcttacgccg ggctcacggt tgatgcgggc catgatgttt ccctgcattc
    19561 tggcggcatc ttcttcatcc ataagatcgt aaatatgtaa attaaccgct tcttcattgg
    19621 tgtatcccaa tacacgggtg gctgcagggc tggcatatac aaaacgcaga tccgtattca
    19681 cctgccagac gatctcatcc acgtacgagg tgatcaggcg gtagcgctct tcactggctc
    19741 gcaatgcctc atctgccatc tttctttcgg tgacgttttc atgagcaagc acgatacgca
    19801 cctggccacg gtccataaat tttgtaatat ggacgacata ccaacgcttc tcttccaaac
    19861 cgtggcatgg gtactcaacc caggcggctg ttttctcccc ggaacagacc gcgcgaatct
    19921 cctgggcaac ctgttttgct tcactggcgt ctgcaccttc tgctgaatca caaacctgta
    19981 gataattcat cccaatacag gcatcggggc gatccaaccc attttctttg ccaaattttc
    20041 tccaggctga gttgacctgt agaataaccc cgttttcatc caaaatcgca agatgcgccg
    20101 acaaggcatc ctgcaccccc tgaaggaatt cgcgcgcgct ttgcaggtct tcctgagcct
    20161 gcttgtactc ggtaatatcc tgaatggtac ccatcatgcg gatgggctgg cctctatcgg
    20221 aataaacaat ctttgctttt tgatggatcc aacgccttcg accgccgctt aaattgatac
    20281 ggtattcgta gtctgcatca gaatgtgtgc taaatgtggc atgttcgagc tcacgcagac
    20341 gccccacttc ttctgcgggt attatgcttg taattatctg ttgagaaatc tggtcgcctt
    20401 tttgcatgtc gaaaatattg tagatctcat cggagcatat gagtttatta tcaggcgatg
    20461 tccattccca atgcccaata tggccgattt tttgtgcctc ggccagcaat gcctggcttt
    20521 ttagcaaagc ttcctgagcc agtttctgtt cggtaacatc ctgcacggtg ccaatgatac
    20581 gggtgatatt cccgtttttg tctttgatca gttgtgtttt ctctgttaca tgacgaaccc
    20641 gcccatcagg caggatgata cggtggtcaa tggacgcaga ttgttgttcc t
//
LOCUS       DAOQLC010000077        20556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_903_length_20556_cov_20.595825, whole genome shotgun sequence.
ACCESSION   DAOQLC010000077 DAOQLC010000000
VERSION     DAOQLC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_903_length_20556_cov_20.595825"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="PL000_13680"
     CDS             <1..2214
                     /locus_tag="PL000_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90000.1"
                     /translation="NFIEIKQDVTEQKILQEQLQKQNDYLSILHQVTLDLLNRRDLTD
                     LLKTIVNHSALLLDAPFSELMLVEGDKLVVKAVTDNQLDILEEKVGRDQASLSWKAFD
                     TLQPVLLEDYSTWSGHRPKYDDFQLHAVADFPVVAGDHCFGVLALGRNKPDYPFTPEQ
                     TQTGILFARLVALVLDNVNLYDSAMQEIMERERVQASLQRSNQQQQVINSLLKVSLEN
                     RSMDEVLGSILDEILSIDWLTISPKGGVFLFNKQTNLLELRTHRNLSPHLQSLCAGVA
                     LGHCLCGRAAQTRQIQFADCLDERHETRYEGIEEHGHYNIPIVDGEDILGVIVLYLPH
                     GYQKSENDLVFLNSVADAVKGIMQRKQAETLLLESEIRFRQIVENASDIIYRADINGN
                     FTYANPSALKMMGFMSEKDVIGKNYLDLTTSEYRQKLKRVYDHQYISKTMSTYHEFPA
                     VAADGGIVWVGQNVQLIMDGDKIIGFQAVARNITQLKQAQEALALSRDQALEASRFKS
                     QLVSRVSHELRTPLGGILGYAELLQVEAFGGLNEKQQAALKNIVESTNFLTTTVNDLL
                     DQAQIESKSLSLHNAYFSPATLLSKISAPMSVLAEKKGLKFRTEISPSLPLELYGDEK
                     RLQQVIINLAGNAIKFTSAGEVYINVSSPIPAYWAIEVRDTGVGIPENEQANIFEPFR
                     QVSNSITRENRGSGLGLAIVRQLVELMGGQISLQSESGKGSIFTVTLPLINAPGE"
     gene            2217..2573
                     /locus_tag="PL000_13685"
     CDS             2217..2573
                     /locus_tag="PL000_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90001.1"
                     /translation="MPIQPYAIVVEDDPKLGAIFQTTLQHAGFETALDHNGDKYQAFL
                     NIAEPALVILDLHLPFAFGGDILEVIRARYPNTVVAIVTADLYKAKAFNGKADHILIK
                     PVSVASLLKLAESVKG"
     gene            2583..2945
                     /locus_tag="PL000_13690"
     CDS             2583..2945
                     /locus_tag="PL000_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90002.1"
                     /translation="MPKPLALVIEDDPQLNQIFTLTLFESFEVETISDGSQALDRLTE
                     IIPNVVVLDINLPGVPGDQILRMIRSNSRLHSVKVILATASTHQARDLQDMADIVLLK
                     PISPMQLRELAERLSRMG"
     gene            complement(2982..3485)
                     /locus_tag="PL000_13695"
     CDS             complement(2982..3485)
                     /locus_tag="PL000_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HNC90003.1"
                     /translation="MKNSCSSPLHPQAIEGLRLFNQRKYFEAHEALEDAWREETGPVR
                     ELYRGILQVAVAYLHITRGNYEGAVKVYGRSLKWLKDWPDICRGIGVEQFRKDAERVM
                     NQVVILGKDGIRNFNPSLFHEITWNEQRVWICDRCGTEMYEKNCKVSCPNCGNRFDCS
                     DLNIYFD"
     gene            3544..4437
                     /locus_tag="PL000_13700"
     CDS             3544..4437
                     /locus_tag="PL000_13700"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNC90004.1"
                     /translation="MRKQLEDRRWGIALAFFALISLVLLAGALQNANFLPAQPLGGSE
                     SDQITIDLSVLKDALVNSGEIPLWKQFVFFGAIFLFLSLLALFLSPELRKKLFLMLLR
                     LLASVIMVYYLLKVKPDIFSGLLQFGQLGQSDGQIQDVPAPVFQPPQISSATTFLLTL
                     AFVVLVAFLFWLINRWWTRQKELIAKRRPLAEIAAIARASLHDLASASNSNDAIIQCY
                     VRMSNVVAMKRGLQRQYAMTPAEFAAYLEGAGIPPEPISRLTGLFEAVRYGGHISGPR
                     EIDEASDCLKSILRYCGEAVS"
     gene            4434..5042
                     /locus_tag="PL000_13705"
     CDS             4434..5042
                     /locus_tag="PL000_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90005.1"
                     /translation="MKRRYLLLALLMVGAVVVSYLLKEVIYQTVFVPLAYLWWLFGLY
                     YRAVPQLILWILLVVMVFVSSLKLVPIINFFPAVEKKIKNETVGPVEQLSMMLNKPSR
                     GVYYKWMIANRLGRVAREMLDQREGRTFKGYSRLRGRDWTPPADVDEYLETGLNGSFA
                     DYPTPHWWREAEPTPLDLDPQKAIDYLENEMEMNRDRNRKSI"
     gene            5017..5970
                     /locus_tag="PL000_13710"
     CDS             5017..5970
                     /locus_tag="PL000_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC90006.1"
                     /translation="MTEIVKASELSKQVISEVERAIVGKRSLLEMIMAAVLAGGHVLL
                     EDYPGLGKTLIARSFAAALGLDFKRIQFTPDLLPGDITGGYIFNRTKNKFELRQGPVF
                     TNILLADEINRASPKTQSALLEAMQEGQVTLEGESMKLPDPFIVLATQNPIEYEGTFP
                     LPEAQLDRFMLKLTVGYPDLEEEREILKRRRERKQDELALKQVIKPKQLLEMRAALES
                     VHVDSDLEGYISALVHATRTDRRVAVGASPRGSLAFLKMARASAVLAGRDFILPDDIK
                     RFAVPVLSHRLLLQPEYWMSQQVNGDVIRDVLSHTPVPVIK"
     gene            5972..7252
                     /locus_tag="PL000_13715"
     CDS             5972..7252
                     /locus_tag="PL000_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC90007.1"
                     /translation="MYRYVLLSALVYGLLLAGLATLQGTFVALAIPFMLFLVYGFLNA
                     PEELNIQIQRTLSTDRASADADVVVTLKVTNNGAAIEELLLDDRISPNLTLRNGSPRH
                     LLRLAKDSTCTLTYSVAGTRGMYHFDQIHLTVTEPLGLIRREQTVHAEKRLTIFPLVT
                     KFKSVAIHPLRTRVYAGTIPGRIGGPGTEFFGVREYQMGDSPRTINWRASARHPGNLF
                     SNEYQQERVADVGVVLDARASANIFHVDQSLFEYSVAAAAGLADAFLAQGNRVGLLVY
                     GSYLGWTMPGYGKIQRERIMRALAGAEVGVSSVFSGLQHLSPHMFPPESQIVLVSPLV
                     NDDLEVLIQLRGRGYQVMVISPDPARFESRLLADHPKVDLAERIVRMERSLLLRRLER
                     AGIQVIGWDVSIPLDQAIGPFLTRPHRSVFRRSS"
     gene            7249..7737
                     /locus_tag="PL000_13720"
     CDS             7249..7737
                     /locus_tag="PL000_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90008.1"
                     /translation="MTEFVLVASIVLSAGSLAWVFFEAGLSDFGRWVLIFGVAWLVAQ
                     RVGWKWISSVGLVLAVLLAATGLWFGLTPGWMFAGAIFALAAWDLTDFRDRLRFVAAD
                     DNARGMERRHLARISWLVLVGMALASITMVLQVKFTIEWGALLVVVILIGLGQLVNWL
                     RK"
     gene            complement(7764..8312)
                     /locus_tag="PL000_13725"
     CDS             complement(7764..8312)
                     /locus_tag="PL000_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90009.1"
                     /translation="MKPFPYRKVVIVGTTSSGKSTLARALAEKIGAICIDLDYLHWEP
                     NWVEAPNDIFRERVKKAISAESWVVAGNYSATRDLIWPQAEAIIWLNYSFPIVFWRLF
                     TRTFRRVATKEKLFSDNVENFWVQIKFWSQDSLFNWLFKTYWRRKREYPNLFSQPENA
                     HLTIIQFEHPQEAEKWLAAISA"
     gene            8410..9759
                     /locus_tag="PL000_13730"
     CDS             8410..9759
                     /locus_tag="PL000_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC90010.1"
                     /translation="MFELPDDEILNLSKEHVFWTWSAQAKVNPIAVKKAKGVYFWDVD
                     DKKYLDFNSMTMCVNIGHGNERVIKAMQEQAAELPYAAPGMATKIRALASKAVAEITP
                     KGALSKVLFTLGGADANENAIKLARGYTKRHKILTRYRSYHGASAGAMAATGDPRRVA
                     WEPNLMTGVVHFLDPYRYRSTFHRTNPNISEEEFTRDYLNHLEEIILYEGPESIAAIL
                     MESVTGTNGIIIPPNGYMQGVRALCDKYGIVMICDEVMSGFGRTGKMFAIEHWDVVPD
                     IMTMAKGLTSAYAPLGAVAMKPEIAAAFNEHAFESGLTYTSHPISLAAAVANIEVMKE
                     ERIVENAAAMGATLRRMLTDLGEAHPSVGEVRNIGLFGILELVKDRSTKEPMAPWNGS
                     SPEMNALKKYCTDHGLFLYTHWHTVLIIPPLIITEEELKEGMDVLDKALEITDKVVK"
     gene            complement(9777..10757)
                     /locus_tag="PL000_13735"
     CDS             complement(9777..10757)
                     /locus_tag="PL000_13735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC90011.1"
                     /translation="MLFIAGTAAILYWAFSEYAGVRNIWLLLCCTPVAVIMLGVFAVF
                     SMYMRFGKPLSQIFNAIDSVAEGDLSVRVPENRSDMFSELITRFNKMIAELERADQQR
                     RNLTSDIAHELRTPLHIIQGNLEGIIDGVYEPTTEHINNTLDETKLLARLVNDLQTLS
                     LAESGQLPLHPTRFLLADLTHDLTASFSAQAATLGIDLTTELANPTQEITADYDRLNQ
                     VLSNLISNALRHTPKGGTISLETESGLSEAGSVRIKVKDSGEGIASEDLPFIFDRFWR
                     GDKSRTGRVHSGLGLAIAKQIVHAHNGTIEVESEIGRGTTFTIEIPTGHV"
     gene            complement(10877..11632)
                     /locus_tag="PL000_13740"
     CDS             complement(10877..11632)
                     /locus_tag="PL000_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90012.1"
                     /translation="MGDLEKVIALPIFILHLSPFIPYTAPMSEIILVVDDEPKITRLA
                     RDYLEKNGYHVLTASDGQSALATARREKPDLIVLDLMLPNMDGREVCKILRRESDVPI
                     IMLTALAEEIDQVTGLEIGADDYITKPFSPRALVARVRALLRRSKGGIKTPTTIRIGG
                     LEIDSEKYSVTLNGTPIKLTPNEFKLLSILASRPGQTLTREQLLEDLHGAASSIDRSV
                     DSHIKNLRKKLEAESDSPSIETIYGIGYRFIER"
     gene            complement(11611..12417)
                     /locus_tag="PL000_13745"
     CDS             complement(11611..12417)
                     /locus_tag="PL000_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC90013.1"
                     /translation="MNRNDILIEADELLKKLGDKNIRIFDATITDDAYLQEHIPGAAF
                     LEHENFSAPESANRYTTMLPEDKLAEQIGALGISNETEVVVYACGMLPYAVRAWWVLR
                     YAGHDKVRILNGGLSAWKQAGGQTEQEIHSYEPANFQPSLRKWMYADKEEVLKSLKET
                     DIAVVNVMPRESYDARHIKGSICLSSMNLMQQLDAFLPDDQLAAHLQEVTHNKRVITY
                     CGGGIAATVNAVAHLMTGYEDVTVYHGSMFEWLAEDLPTVGDGKWEIWKK"
     gene            complement(12601..13089)
                     /locus_tag="PL000_13750"
     CDS             complement(12601..13089)
                     /locus_tag="PL000_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90014.1"
                     /translation="MKKGPFILFRVIGFLLVLGLIAGAGAFAYKAGMAQGISQAPAVA
                     TAIAQAAEDGQAVPPMVYGHGFAPYGYGYGMHGHGHFGFFPLGGICFGIFLLFFFFGF
                     MKMMFFRRMRHGWGHHGGHGPWGKNWEGGVPPMFNEWHKRAHGETPAADGEKPAEEKK
                     SE"
     gene            13365..14303
                     /gene="trxB"
                     /locus_tag="PL000_13755"
     CDS             13365..14303
                     /gene="trxB"
                     /locus_tag="PL000_13755"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC90015.1"
                     /translation="MTEKHVKVLVLGSGPAGLSAALYAARAELEPIVLTGMQLGGQAA
                     LTHLIENYPGFPEGVGGAQLGELFQKQAEHFGAKVEFNMAHEVDLSQRPFTVKTDDGD
                     YKAETLIVTTGANPNHLEIPGEVELTGRGVSYCATCDGWFFKDKKVVIVGGGDSAFEE
                     ALFITRYASSVTLIHRREDFRAGAILQKRAKEHPKMNFILNTVVTEVLGSDKLEALKL
                     KNVQTGEESIFETDGLFIFIGHTPNSQMFKGQLEMSDLGYVLVNDKMEASVEGVYAAG
                     EIADPHFRQVITSAGMGAAAAIQATKFLEEKEAVHS"
     gene            14527..15462
                     /gene="mdh"
                     /locus_tag="PL000_13760"
     CDS             14527..15462
                     /gene="mdh"
                     /locus_tag="PL000_13760"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC90016.1"
                     /translation="MKKISIIGAGNTGATAAHWLAERELADVVLLDVVEGMPQGKSLD
                     MLEAMPIIGKDTHVVGTNDYADTKNSDIIIITAGIARKPGMSRDDLLKTNADIVGKAA
                     METLKHSPNAIFIVLTNPLDTMAYLTMKMTKLPRERVIGQAGILDSARMRAFVAMETG
                     VSVENINCYVLGGHGDEMVPLTRHSNIAGIPLKEYLPADKLEAIVNRTRKGGGEIVNL
                     LKTGSAYYAPSMACVQMAEAILKDKKLIVPCATMMQGEYGLNDMYFGVPVVLGAGGME
                     RVVEYKFDAEEKAMFEKSAASVKETHEALKGLVTF"
     gene            15510..16829
                     /locus_tag="PL000_13765"
     CDS             15510..16829
                     /locus_tag="PL000_13765"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HNC90017.1"
                     /translation="MILHEKLSAQLPAWRERIKSLAKEHAEVVVDHVNIGQIVGGMRD
                     IKSLLSDVSFVDPAHGIIFRGLSIPELLKALPKARASKMPLVGGLYYLLMIGEVPTKE
                     QALEVEAEWAKRATVPDYVYKMLKTMPKETHPMTLFSQAVLALQNGSNFAAKYHSMKK
                     DVYWEAALEDSLDLTAKLPVIAAYIYRMKYYGETSKPKYNPKLDYGANFSKMMKVSDK
                     KGYSELARLYFILHSDHEAGNVSAHTMHLVGSALSDPYLSFSASLNGLAGPLHGLANQ
                     ECLGWLLEVHKKFGGVPSREDLYKFAWDTLNGGHVIPGYGHAVLRVPDPRFMAQMEFA
                     KKRFPDDELVRLADLVFDVVPAVLKEQGKAKNPAPNVDAISGTLQYYYGVREFDFYTV
                     LFGVGRALGVTANYVWARALGMPIERPKSLTTKMLEDVVAKAAQQPA"
     gene            complement(16900..18957)
                     /locus_tag="PL000_13770"
     CDS             complement(16900..18957)
                     /locus_tag="PL000_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNC90018.1"
                     /translation="MPPIAPKRDHALTQHGQTRNDEYYWMRNREDPEVKAYLQAENDY
                     LEETLQHLNPLREKLFHEMKARIREVDHSVPEKHGDFLYYTRTEAEKQYPIFCRKKNI
                     PNAAEEILLDQNALAEGHEFCSVSAFSVSPDQNKLAYSVDLEGSEVYTLHVKDLISGT
                     MYPEAIANIHGSVYFQAGVEWANDNETVFYLSLDESHRACKLYRHKVGTAPSQDVLVY
                     FEEDDTFSLSLFKSRSEKYIFTYHYNTISQEMRFLPADHPVGALNVLQPRKAGLEYYA
                     AHLDDHFYIVTNEDAHNFKLMKTPTAAPSRENWQEVIGHREDTLLEHVDVFAEHIVLH
                     ERKGGLKQLRVSRPDGHSDVRYVNFPDPTYEVIVDVNPEFHTNLLRLRYSSLTTPFST
                     VDFHMDNGEWEVKKEYQIPGGYDKSQYVTEYIHATAADGRKIPISLAYKKGLKKDGQN
                     PTLLYGYGAYGASSDAAFNLSLLSLLDRGFVFAIAHVRGGSEMGRFWYDEGRMFNKKN
                     SFTDFIACAEHLIAEGFTSPEKLASTGGSAGGLLVGASMVMRPELFEAVICKVPFVDV
                     ITSMSDPSIPLTTLEYDQWGNPANRDEYDYMLSYSPYDNLREVEYPHLLLSTGFNDPR
                     VAYWEPAKFLARLRDKKNDDNLALLQTNFSAGHAGASGRYDYLKENVLDFAFLIDRLG
                     AKP"
     gene            complement(18974..>20556)
                     /locus_tag="PL000_13775"
     CDS             complement(18974..>20556)
                     /locus_tag="PL000_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90019.1"
                     /translation="GWRCFVSLLLSLPCDIIPQLMEKHSPLKIILITSAVFYLIFIIG
                     TSFDIKGERYFTLVDDAMISMRYARNLAQGHGLVWNIGEAPVEGFTNLGWTLYLAFLH
                     LFPFPASKISLVVMLTSLAILLANSYTVSKIAEALQPDSKYAPTLAALITAFYFPLVF
                     WSLRGMEVGLLALLVSLAVLFSITEKHPVFIGLIFAAAILVRVDAIISIAPIALYLLL
                     KNKRNAILPIASAAITLLAVFYFQHNYFGDFLPATYYQKMTGFTALERIRHGLLVFVE
                     FAARDTLPLFLFSLAGIFFYKLQRSRETLLLLGIFLAQSAYSIYVGGDYAEPETNAAN
                     RFITQGMPALIILFSWMTSRILTDLQAAQPQSAFTAPKVNPAIPLAVITLLIVSGEPW
                     FTYTVDNAPLLKADIRRVRLGLFLAESTSPEAVIAVHAAGQIPYYSQRTTIDLLGLND
                     PIVAKGQGHGEFYPGHNKWDYDYSIIHLRPDLIADNFAPLADFMRGKEEYQKLENGIY
                     IRIDSNLINLEEISQDYR"
BASE COUNT         4692 a         4935 c         5731 g         5198 t
ORIGIN      
        1 aattttatcg agatcaagca ggatgtgacg gaacaaaaga tcctgcagga gcaactgcaa
       61 aagcagaatg attatctatc cattcttcat caggtaacac ttgacctgct caatcgccgc
      121 gatctaacag accttttgaa gacgattgtc aatcattccg ccttgttact ggatgcgccc
      181 tttagcgaat taatgctggt tgaaggcgac aagttggtag tgaaagcggt gacggataat
      241 caactggata tcctggagga aaaggttggc cgcgatcagg catctctttc atggaaagca
      301 tttgatacgc ttcaacccgt cctgcttgaa gattattcga cctggtcagg gcacagacct
      361 aaatatgatg attttcaatt acacgccgtg gctgattttc ctgttgtggc aggagatcat
      421 tgttttggtg tgcttgccct tggcaggaat aaacccgatt atccctttac ccctgaacag
      481 acccagacag gtattttgtt tgcccgcttg gttgcactcg tgctcgacaa tgtgaatctc
      541 tatgactcag ccatgcagga gatcatggaa cgcgaacgtg tacaggcatc cttgcagcgt
      601 tcgaaccagc agcaacaggt catcaactcg ctcttgaaag tgagcctcga aaatcgctca
      661 atggatgaag tcctcggctc cattttggat gagatccttt caattgactg gctgaccatt
      721 tctccgaaag gcggcgtttt cctgttcaat aagcagacca acttgttgga acttcgaacc
      781 caccgcaatc tttcgccgca tttacagtca ttgtgtgccg gtgtggcttt ggggcattgc
      841 ctgtgcggac gcgccgcgca aacccgccag atccagttcg cagattgcct tgatgaacgc
      901 cacgagactc ggtatgaagg catcgaggaa catggccatt acaacattcc cattgttgat
      961 ggcgaagaca tcctgggggt gatcgttttg tatctgccgc atggatatca gaaatcggaa
     1021 aatgaccttg tcttcctgaa ttcagtggca gatgctgtga agggtatcat gcagcgcaag
     1081 caggcggaga ccctgcttct ggaaagtgag atccgtttcc gtcaaattgt tgaaaatgcc
     1141 agcgatatca tttatcgtgc cgatatcaac ggcaatttca catatgccaa tccatccgca
     1201 ttgaagatga tgggctttat gagcgaaaaa gatgtgatcg gaaaaaatta tcttgatctg
     1261 accacatctg aatatcgtca aaaattgaag cgcgtgtacg atcatcaata catcagcaag
     1321 accatgagca cgtatcacga attccctgcc gtggctgctg atggaggtat tgtttgggtt
     1381 gggcaaaacg ttcaactcat catggatggc gataagatca tcgggtttca ggctgtggcg
     1441 cgtaacatta cccagcttaa acaggcacag gaagccctgg ccttatctcg cgatcaagcc
     1501 ctggaggcca gccgtttcaa gagtcagctt gtctcaaggg tcagccatga attgcgcact
     1561 ccattgggcg gcattcttgg ctacgctgag ttacttcaag tggaagcctt tggcggactt
     1621 aatgaaaaac aacaagccgc gctcaaaaat atcgttgaga gcacaaactt tcttaccacc
     1681 acggtcaatg acttgctcga tcaagcccag atcgaatcca aatcgcttag cttgcacaat
     1741 gcttatttca gcccggcgac attgctctca aagatctctg cgcccatgag tgtgttggct
     1801 gagaaaaagg gattaaaatt ccgcactgaa atttcaccat ccctgccgct ggaactgtat
     1861 ggtgatgaaa aacgcctgca gcaggtcatc atcaatcttg ctggcaatgc gatcaagttt
     1921 acgagcgctg gtgaagtcta cattaatgtc tcatctccca tccccgctta ttgggcgatc
     1981 gaagtgcgtg atacaggagt aggcatcccg gaaaacgaac aggcaaatat ctttgagcct
     2041 ttccgccagg tcagtaattc catcacacgc gagaaccgtg gttcaggtct ggggcttgcc
     2101 atcgtaaggc aattggtgga attgatgggc ggccaaatat ccctgcaaag tgaatcaggc
     2161 aaaggtagta tctttactgt taccctgccg cttattaatg ctcctggaga atagtgatgc
     2221 caattcaacc ctatgcgatc gttgtggaag atgacccaaa gctcggagcc atcttccaaa
     2281 ccaccctgca acatgcggga tttgaaaccg ccctcgatca caatggcgac aaatatcaag
     2341 cgtttctaaa tatcgccgaa cctgctcttg tgatcctcga tcttcatctc ccctttgcat
     2401 ttggtggcga tattctggag gtgattcgtg cccgctaccc caatacggtc gttgcaatcg
     2461 tcaccgcaga tctttataaa gccaaggctt tcaacggcaa agctgaccat atcctcatta
     2521 aaccggtcag cgttgcgagc cttttgaaac tcgccgaatc agtgaaagga tgaactagtc
     2581 ttatgcccaa accccttgcg ctcgtaattg aagatgaccc acaactcaat cagattttca
     2641 cgctaaccct gttcgaatcc tttgaagttg aaactatttc agatggttcc caagccctgg
     2701 atcggctgac tgaaattatc cccaatgttg ttgtccttga tattaacctg cccggagtgc
     2761 ccggtgacca gattcttaga atgatccgct cgaattcgcg tttgcattcg gtgaaggtca
     2821 tattggcgac ggccagcacg caccaagcca gagaccttca ggatatggcc gatatcgtgc
     2881 ttttgaaacc gatcagcccc atgcagctgc gggagcttgc cgaacggctt agtcgtatgg
     2941 ggtagggttt cgattggcga aattggaaaa ggctgggaat ttcagtcaaa gtaaatattc
     3001 aggtctgaac aatcaaagcg gtttccacaa ttggggcaac tgaccttgca attcttttca
     3061 tacatttctg tcccacagcg atcgcagatc catacccgtt gttcgttcca tgtaatctca
     3121 tgaaaaaggg atgggttgaa gtttcggatg ccatccttgc ccagaatgac gacctgattc
     3181 atgacccttt cagcatcttt cctgaattgt tccaccccaa tacccctgca aatatccggc
     3241 caatctttca gccatttcaa actccgtcca tacaccttga ctgcgccctc gtaatttccg
     3301 cgggtaatgt gcaagtatgc caccgcaact tgaaggatgc cgcgatacaa ctcgcggacc
     3361 gggcctgttt cttcgcgcca tgcatcttca agcgcttcgt gggcttcgaa gtactttctc
     3421 tggttgaaca accgtaaccc ctcaatggct tgcggatgga gcggggagga acaggaattt
     3481 ttcataaaac agttctgtag tatggtttgg gccgtgtctt tcaaatggta gaattaaacc
     3541 acaatgcgaa agcaacttga agacagacgc tggggaattg ccctggcatt ttttgcgctg
     3601 atttccctgg ttttgctggc gggcgcattg caaaatgcaa actttctgcc cgcccagccg
     3661 ttgggtggct ctgaaagcga ccaaatcacc attgaccttt ctgtgctgaa agacgcgttg
     3721 gttaattcgg gggaaattcc tctttggaaa caatttgtat ttttcggggc cattttcctt
     3781 tttctttcac tgttggcgct gtttctctcc cctgagctgc gcaaaaaact gttcttaatg
     3841 ctgctgcgcc tgctggcctc agtgattatg gtgtattacc tgttgaaggt caagccggat
     3901 attttttcag ggctattgca gtttggtcaa ctggggcaat cagacggaca gattcaggat
     3961 gtgcccgcac ctgtttttca acctccacag atatcatcag ccacaacctt tttgttgacc
     4021 ctggcttttg tggttctggt cgcatttctg ttctggctga tcaatcgctg gtggacaagg
     4081 caaaaggaat tgatcgcaaa gcgaagacca cttgccgaaa ttgcagcgat tgcacgcgca
     4141 tcgctgcatg atcttgcctc tgcttcaaat tcgaatgatg ccattattca atgttatgtc
     4201 cgcatgagca atgtggtggc gatgaaacgg ggactgcagc gtcagtatgc catgacccct
     4261 gccgaatttg cggcgtatct tgaaggggca ggcattcctc ccgagccgat ctcccgttta
     4321 acggggttgt tcgaggcggt tcgttatggc gggcatattt caggtccacg ggagatcgat
     4381 gaagcctccg actgtctgaa atccatcctt cgatattgtg gcgaggccgt atcgtgaagc
     4441 gccgctattt attgcttgcc ctgttgatgg ttggagcggt ggtggtctcg tacttgttga
     4501 aggaagtgat ctatcaaact gtttttgtgc cattggcgta cttgtggtgg ttgtttggtt
     4561 tgtattatcg tgccgtgccg cagctgatcc tgtggatttt gcttgtggtc atggtttttg
     4621 tttcctctct gaaactggtt cccattatca attttttccc tgcggtggaa aaaaagatca
     4681 agaatgaaac agtgggcccg gtggagcagc tttccatgat gctcaataag ccgtcccgcg
     4741 gcgtttatta caagtggatg atcgccaacc gtttaggcag ggtggcgcgt gaaatgctcg
     4801 accaacgcga aggacgtacg ttcaaaggat attcgcgttt gcgcggcagg gattggactc
     4861 cgcctgccga tgtagatgaa tacctcgaaa ccgggttgaa cggctctttt gccgattacc
     4921 caaccccgca ctggtggcgg gaggcagaac caaccccgct cgaccttgac ccgcaaaaag
     4981 caattgacta tcttgagaac gagatggaga tgaatcgtga ccgaaatcgt aaaagcatct
     5041 gaactgagta aacaggttat ctctgaagtg gagcgtgcca tcgtgggcaa acgcagtttg
     5101 cttgaaatga tcatggcggc tgttctggcg ggcgggcatg ttttattgga agattacccc
     5161 ggccttggca aaaccctgat cgcccgcagt tttgcggcgg cactcgggtt ggatttcaag
     5221 cggattcaat ttacgcccga tcttttgccc ggcgatatta ccggtggcta tattttcaat
     5281 cggacaaaga acaagttcga gttaaggcag ggacccgttt ttaccaacat cctgcttgca
     5341 gatgagatca atcgcgcttc gcccaagacc caatctgctt tgttggaagc catgcaggaa
     5401 ggtcaagtga ctctggaagg cgagagtatg aaactgcccg atccgttcat tgtgctggcg
     5461 acacaaaacc cgatcgaata cgagggcact ttcccgctgc ccgaagcgca actggatcgt
     5521 ttcatgttga agttgaccgt ggggtacccc gaccttgaag aagagcggga gatcctcaaa
     5581 cgccgccgcg aacgcaagca ggatgaactt gctctcaaac aagtcatcaa gccgaaacag
     5641 ttgctcgaaa tgcgtgccgc gcttgaatcg gtgcatgtgg attctgatct tgaggggtac
     5701 atctctgcgc tggtgcatgc cacacgcacc gatcgccgtg tggctgttgg agccagcccg
     5761 cgcggatcgc tggcgtttct aaaaatggcg cgtgccagtg ccgtactggc tgggcgggat
     5821 ttcattttgc ccgatgatat taaacgcttt gccgtgcccg tgttgagtca tcgccttttg
     5881 ctgcagcccg aatattggat gtcacagcag gtcaatggcg atgtcattcg tgatgtattg
     5941 agccacactc ccgttcccgt cataaaataa cttgtatcgc tacgtcctcc tttctgcgtt
     6001 ggtgtatgga ttgctgcttg cagggttggc caccctgcag ggcacttttg ttgcgcttgc
     6061 gattcctttc atgctgttcc tcgtttatgg ttttttgaat gcgcccgaag agttgaatat
     6121 tcagattcaa cgcacgcttt ccaccgaccg tgcttcagcc gatgcggatg tggtcgtaac
     6181 tttgaaagtc accaacaacg gcgcagcaat tgaggaactt ttactggatg acaggatttc
     6241 tccaaacctg accttgcgaa acggatcgcc ccgacacttg ctgaggctcg cgaaggacag
     6301 cacctgcacc ctgacatatt ctgtcgcagg gactcgcggc atgtaccact ttgaccagat
     6361 tcatctcacg gtcactgaac cgcttggatt gattcgccgt gagcagaccg tccatgctga
     6421 aaaacggttg accatcttcc ccttggtgac aaagtttaaa tcggttgcca ttcaccccct
     6481 gcggactcgg gtttatgctg ggaccattcc cgggcgcatt ggaggcccag ggacagagtt
     6541 ctttggtgtg cgtgaatatc aaatgggcga ttctccccgc acgatcaatt ggcgcgccag
     6601 tgccagacac cccggtaatt tgttttcaaa cgaatatcaa caggaacgtg tggcggatgt
     6661 cggtgtggtg ttggatgccc gtgccagtgc caacatcttt catgtggatc aatcgttgtt
     6721 cgagtattcg gtggctgccg ctgcaggtct tgcggatgcg ttcttggcgc agggcaaccg
     6781 tgtcggcttg ttggtttatg gctcgtatct tggttggacc atgcccgggt atgggaaaat
     6841 tcagcgtgaa cgcatcatgc gtgccttggc aggggcagag gtgggtgtga gctccgtctt
     6901 ttcgggcttg cagcatttat cgccgcacat gttcccgcct gaatcgcaga tcgtgctggt
     6961 tagtccgctg gtaaatgatg atcttgaggt tttgattcaa ctgcgtggac gcggctatca
     7021 agtgatggtc atcagcccag accctgcgcg ctttgaaagc cgccttttgg ccgaccaccc
     7081 caaagtggat ttggcggaac gcattgttcg catggagcgc agcctgttgc tgcgacgatt
     7141 ggaacgggcg gggattcaag tcatcgggtg ggatgtgtcg attccgctgg atcaggccat
     7201 cggtccattt ttgacacggc ctcatcggtc cgttttcagg aggtcatcgt gacagaattt
     7261 gtacttgtgg ccagcatcgt cttaagtgcg gggtcgctgg cttgggtgtt ttttgaagca
     7321 ggcttgagcg attttggacg ctgggttttg atctttggtg tggcatggtt ggtggcacag
     7381 cgggtcggtt ggaagtggat ttcctcagtg gggctggtgt tggcggtcct tcttgcagcg
     7441 actggcctgt ggtttgggct tactccaggc tggatgtttg caggggcgat tttcgccctc
     7501 gccgcatggg acttgaccga tttccgcgac cgcctgcgtt ttgtcgccgc cgatgacaat
     7561 gcgcgcggca tggaacggcg tcatctggcg cgcatcagtt ggctggtgtt ggtggggatg
     7621 gcacttgcct cgatcacgat ggttctgcaa gtcaaattca ccattgaatg gggtgcgctg
     7681 ctggtggtgg tgatcttgat cgggctgggg cagttggtca attggttgag aaaataattt
     7741 tgctttgagc ggtattgctt cgcttatgct gaaatggccg ccagccattt ttcggcttcc
     7801 tgcggatgtt cgaattggat gatcgtcagg tgggcatttt cgggttgtga gaacaggttt
     7861 gggtattcgc gttttctgcg ccagtaggtt ttgaacagcc agttgaaaag cgaatcttgc
     7921 gaccagaatt ttatttgtac ccagaaattt tcaacgttat ctgaaaagag tttttctttt
     7981 gttgccacgc gccgaaatgt gcgggtgaac aaccgccaga aaacaatcgg gaaggaataa
     8041 ttgagccaga tgatcgcctc ggcttgcggc cagatgagat cgcgggttgc gctgtaattt
     8101 cccgccacca cccagctttc ggcgctgatg gcttttttta cccgttcacg gaatatgtcg
     8161 ttgggggctt ccacccaatt gggctcccag tgcaggtaat caaggtcaat gcaaatggcg
     8221 ccgattttct ccgccagagc cctcgccagc gtggattttc ccgatgaggt ggtgccgaca
     8281 ataacaactt tacggtaggg aaatggtttc ataatgggca gagtataatt tggctgtcat
     8341 aaatcgtaaa cttgtgctga acaagtcgaa atatttcaaa tcgaaaatca aaaatccaaa
     8401 tgaggctcca tgtttgaact tcctgatgat gaaattctga atctctcaaa agaacacgtc
     8461 ttctggacgt ggtcggcgca agccaaagtg aatccaattg ccgtgaagaa agccaaaggc
     8521 gtttacttct gggatgtgga tgataagaag tacctcgatt tcaactcgat gaccatgtgt
     8581 gtgaatattg gtcatggcaa cgaacgtgtc atcaaagcca tgcaggagca ggccgcggaa
     8641 ttgccctatg ccgcgccagg catggctaca aagatccgcg cattggcaag caaggcggtg
     8701 gcagagatca cccccaaagg cgcgttgagc aaagtccttt tcacgcttgg cggcgcagat
     8761 gcaaatgaaa acgccatcaa gctggcgcgc ggttatacaa aacggcacaa gatcctcacg
     8821 cgctatcgtt cgtatcacgg ggcctcagca ggagcgatgg ccgcgacagg cgatccgcgc
     8881 cgtgtggctt gggagccgaa cctgatgacg ggggtggtgc atttcctcga cccatatcgt
     8941 tatcgctcca cgttccatcg cacgaacccc aacatttccg aagaggaatt cacccgcgat
     9001 tacctcaacc acctggaaga gatcattctt tacgaaggcc cagaatccat tgcggctatt
     9061 ttgatggaat cggtgacagg cacgaatgga atcatcattc cgccgaatgg gtacatgcag
     9121 ggtgtccgtg cgttgtgtga taaatatggc attgtgatga tctgtgacga agtgatgagc
     9181 gggtttgggc gcactggcaa aatgttcgcg attgaacact gggatgttgt gcctgatatc
     9241 atgaccatgg caaaagggct tacttctgcg tatgcgccgc ttggtgcagt ggcgatgaag
     9301 ccagaaattg ccgcggcttt taatgagcac gcctttgaaa gcggcctgac ttatacatcg
     9361 catcccattt cattggcggc ggcagttgcc aacattgaag tgatgaaaga agaaagaatc
     9421 gttgaaaatg cagcagcgat gggggcaacg cttcgccgaa tgcttactga ccttggcgaa
     9481 gcgcatccgt cagtgggtga ggttcgcaac attggcctgt tcggcattct cgagttggtg
     9541 aaagaccgca gcaccaagga gcccatggcc ccgtggaacg ggtcatcgcc tgagatgaac
     9601 gcgcttaaaa aatattgcac cgatcatggc ctgtttttgt acacccactg gcataccgtc
     9661 ctcatcattc cgcctttgat cattaccgag gaagaactaa aagaaggaat ggatgttctg
     9721 gacaaggctt tggaaatcac cgacaaagtt gtgaaataaa aaaaacaggt cacgtttcaa
     9781 acgtgacctg ttggtatttc gatcgtaaat gtcgtccccc tgccgatctc gctttcgact
     9841 tcgatcgtcc cgttgtgcgc gtggacgatc tgttttgcaa tggccagccc cagcccgctg
     9901 tgaacccgcc ctgtgcgtga tttatctccg cgccagaatc ggtcaaagat gaaaggcaga
     9961 tcttcggatg caattccctc gcctgaatct ttgaccttga tccgcacgct tcccgcttca
    10021 ctgaggcctg attctgtttc aagagaaata gtcccacctt tgggcgtgtg gcggagggca
    10081 ttggaaatga gattcgacag cacctgattc agcctgtcgt aatcagcggt gatttcctgg
    10141 gttgggttgg cgagttcggt ggtcaggtca atgccaaggg tggcggcttg tgcggaaaaa
    10201 cttgcggtga gatcatgcgt caggtcagca agcaggaaac gggtggggtg aagcggaagt
    10261 tgtcctgatt ctgccagtga gagggtttgc aggtcgttga ccaggcgggc gagaagtttg
    10321 gtctcatcca gagtgttgtt gatgtgctcg gtcgtcggct cgtacacgcc atcgatgatg
    10381 ccttcgaggt tgccttgaat aatgtgcaga ggtgtgcgga gttcgtgggc aatgtcagat
    10441 gtaaggttgc ggcgctgctg gtcggcgcgt tcgagttcgg cgatcatctt gttgaagcgg
    10501 gtgatgagtt cgctgaacat gtctgatctg ttttctggca cacgcactga aagatcgcct
    10561 tcggcaacag agtcgatcgc gttgaatatc tggctcaatg gtttgccgaa acgcatgtac
    10621 atggagaaga ccgcaaaaac accgagcatg atcactgcaa caggcgtgca gcataaaaga
    10681 agccagatgt ttctaacccc tgcatattcc gagaaggccc agtacaggat ggcggctgtg
    10741 cctgcaatga aaagcatgac agtgccaaga aagaagaacg cgaacggaaa aaaacgacgc
    10801 cctccgcgcc agcggcgttg gaaatgaggc ggttgatttt ggggagggct ttgatgagac
    10861 atgcgaggtg tgtgggttac cgttcgataa accgatagcc aatgccataa atggtttcaa
    10921 tggatgggct gtctgattcg gcttccagtt tctttctcag gtttttgatg tgcgagtcaa
    10981 cgctgcggtc aatgctggat gcggcgccgt gcaggtcttc gagcaattgt tcgcgggtga
    11041 gcgtttggcc cgggcggctg gcaaggatgg agagcagctt gaattcgttg ggcgtaagtt
    11101 tgatgggtgt tccgttgagt gtgacggaat acttctctga atcgatctcc agcccgccga
    11161 ttcgaattgt ggtcggggtt ttgatgccgc ctttgctgcg gcggagcaag gcacgcacac
    11221 gggcaacgag cgcacgcgga gaaaatggct tggtgatgta atcatcagcg ccgatttcaa
    11281 ggccagtgac ctggtcaatt tcttcggcga gggcggtcag catgatgatg ggaacgtcgc
    11341 tttcgcggcg caagatcttg cagacctcgc gcccgtccat gttgggaagc atgaggtcaa
    11401 ggacgataag gtcgggtttc tcacggcggg cggtggccaa cgccgattga ccgtcactcg
    11461 ccgtgaggac atgatatccg ttcttttcaa gataatcccg tgcaaggcgg gtgatcttgg
    11521 gttcgtcatc tacaacaagg atgatttcag acatgggggc agtataaggg atgaagggtg
    11581 aaagatgaag gatgaaaatt gggagggcga ttactttttc cagatctccc attttccatc
    11641 gccaactgtc ggaaggtctt ctgccagcca ttcgaacatg gagccgtgat acacagtgac
    11701 gtcttcgtac ccagtcatca ggtgggccac tgcattgact gttgcagcga ttcctcctcc
    11761 gcagtaggta atgacacgct tattgtgtgt cacttcttgt aagtgggcgg caagttggtc
    11821 gtcgggcagg aaggcgtcca gttgctgcat cagattcatg gaggaaaggc agatcgagcc
    11881 tttgatgtgc ctggcgtcgt agctttcgcg aggcattacg ttcaccacgg cgatgtcggt
    11941 ttctttcaga gacttcaata cttcttcctt gtctgcgtac atccactttc tcaggctggg
    12001 ctggaaattg gcaggctcat aactgtggat ttcctgttcg gtttgcccgc ctgcctgctt
    12061 ccatgcagag agtccgccgt tcaggatgcg gaccttgtca tgccctgcgt agcgaagcac
    12121 ccaccaggct ctcacggcat atgggagcat tccgcaggca tagaccacca cctctgtttc
    12181 attggaaatg ccaagagcac cgatctgttc ggcgagtttg tcctctggca gcattgtggt
    12241 gtaacggttg gcgctttcag gcgcagagaa attttcatgc tcaaggaaag cggcgccagg
    12301 gatgtgctcc tgtaggtagg cgtcgtctgt aatggttgcg tcaaagatgc ggatattttt
    12361 atcgcccagt ttcttcaaca gttcatcggc ttcgatcaga atgtcatttc ggttcatggc
    12421 agtgcctcca ttggatttag tgttatcgaa actatatcaa cgctttgtgg aggaaatatg
    12481 gacgtctccg ccagaaaatg tggaagttta gacaggagaa aagaaaaaag aggaaagaag
    12541 atgggcttac attcttcttt cctctttctc ttgccgatca ctttttacag atcgtcggtc
    12601 ttactcgctc ttcttttctt cagcgggttt ttcgccgtca gcggcaggag tttcgccgtg
    12661 agcacgtttg tgccactcgt tgaacatggg cgggacgccg ccttcccagt tcttgcccca
    12721 cgggccgtgg ccgccatgat ggccccagcc gtggcgcatt ctgcggaaga acatcatctt
    12781 catgaagccg aagaagaaga acagcaggaa aatgccgaag cagatcccgc cgagcgggaa
    12841 aaagccgaag tgtccatggc catgcattcc atagccgtag ccataaggcg caaagccgtg
    12901 accatacacc atgggcggta cggcctgccc atcttcagcg gcctgtgcga tggcggttgc
    12961 cacagcaggg gcctgtgaaa ttccctgtgc catgcctgct ttataggcaa aggcacctgc
    13021 gccagcgatc agcccgagaa cgagcagaaa gccaatgacg cgaaacaaaa taaacggtcc
    13081 ttttttcatt tttacctctc agagttgaat tgatttggtt tgttcttacg cccctaattt
    13141 atcaatccat tgtggaggaa ttatggatgg gtgggcgaga gattgtggag gctgtgtagg
    13201 aaaagtgtta actggagcgc ctccagcctg agactttgca acttttcctg cgggcacgca
    13261 tctgataaaa tgcaggtcgg taatagcagg cctgaatggg tcgtcttgta tacggttgat
    13321 aacaaatttc aggtgaaaat caccttagga caggaaaacg aaaaatgaca gagaagcatg
    13381 taaaggtatt ggtattgggt tctggccctg ctgggctttc ggcggcgctg tacgcagcgc
    13441 gggcagaatt ggagccgatc gttctgacag gaatgcagtt gggcggccag gccgctctga
    13501 cgcacctcat tgaaaactac cctggttttc ctgaaggtgt gggcggtgcg cagttgggcg
    13561 agttgttcca aaagcaggcc gaacatttcg gcgcgaaggt tgagttcaat atggcgcacg
    13621 aagtggatct ttcccagcgc ccatttacgg tcaagaccga cgatggtgat tacaaggccg
    13681 aaaccctcat tgtgaccacc ggagcgaacc caaaccattt ggagatccca ggtgaggtgg
    13741 aactgactgg tcgcggcgtt tcctattgcg ccacctgtga cggctggttc ttcaaagata
    13801 aaaaggtcgt cattgtgggc ggcggcgata gcgccttcga agaagcgctc ttcatcaccc
    13861 gctacgcttc ctctgtcacg ctcattcatc gccgcgaaga tttccgcgcg ggcgccattt
    13921 tgcagaaacg cgccaaagaa caccccaaga tgaattttat tttgaacacg gtcgtcactg
    13981 aagtgctggg cagtgacaag cttgaagcct tgaagctcaa gaacgtgcag acgggcgagg
    14041 agtcgatttt tgaaactgac ggcctgttca ttttcatcgg tcacaccccc aattcgcaaa
    14101 tgttcaaagg tcagcttgaa atgagcgacc tcggctatgt tctggtaaat gacaagatgg
    14161 aagccagtgt cgaaggtgtg tatgcggctg gcgaaattgc cgacccgcac ttcaggcagg
    14221 tcatcacttc ggcgggcatg ggtgccgctg ccgccattca ggcgacgaaa ttcctcgagg
    14281 aaaaagaagc agttcacagt taagggcgaa gcgatgaaaa tcgcgcttcc actttgacgg
    14341 gtggcctgat tctgaccgaa gggcagtctc tgacgggtgg aagttcctca ataaagactc
    14401 gtgaaagcgg gtcttttttg tgcgtaaaaa aagtgacatt ttgtactttt gacccatgcc
    14461 actttcggat agaattagtg aaaaatcacg gcgtttttcg ccgaccaaac cccattggag
    14521 gaacacatga agaaaatctc aattatcggc gctggcaata cgggcgcaac cgccgcgcat
    14581 tggctcgccg agcgcgaact tgctgatgtt gtcttgctgg acgtggtgga gggaatgccg
    14641 cagggcaaaa gcctcgacat gttggaggcc atgccgatca tcggaaaaga tacgcatgtg
    14701 gtcggcacga acgattatgc cgatacaaag aattcagata tcatcatcat tacggctggc
    14761 attgcccgca agcctggcat gagccgtgat gaccttctga agaccaatgc cgacattgtt
    14821 ggcaaggccg ccatggagac cctcaagcac tctccgaacg ccatttttat tgttttgacc
    14881 aatccgctcg acaccatggc ttatctcacc atgaagatga ccaagctgcc gcgtgaacgt
    14941 gtcatcgggc aggcgggcat tttggattca gcccgcatgc gtgcctttgt tgccatggaa
    15001 actggggtga gtgtggagaa tatcaactgc tatgtgttgg gcggccacgg tgatgaaatg
    15061 gttcccctga cccgtcattc caacattgca ggcattcctt tgaaggaata tctgcccgct
    15121 gacaagcttg aggccattgt caaccgcacc cgcaagggcg gcggcgagat cgtgaacctg
    15181 ctcaagacgg gttccgctta ctatgcgcct tccatggctt gtgtgcaaat ggctgaagcc
    15241 attttgaaag acaagaagtt gatcgtgcct tgcgccacca tgatgcaggg tgaatacggt
    15301 ttgaacgata tgtactttgg cgtgcctgtg gtgctgggcg caggcggcat ggaaagagtc
    15361 gtcgaataca agttcgatgc cgaagaaaag gccatgttcg agaaatctgc ggcttctgtg
    15421 aaggaaacgc acgaagcatt gaagggtttg gttacattct aatttgatgg gtcacggctt
    15481 ttgccgtgac cctacctttg gaggaacaaa tgattttgca tgaaaaactc tctgctcaac
    15541 tgcccgcatg gcgcgaacgc atcaagtctc ttgccaagga acatgccgaa gtggttgtgg
    15601 accatgtcaa catcggccag atcgtgggcg gcatgcgcga tatcaagtcc ctgctttctg
    15661 atgtctcttt tgttgacccc gcacacggga tcatcttccg cggcttatcc atccctgagt
    15721 tgttgaaggc gctgcccaag gcgcgcgcca gcaaaatgcc tttggtgggc ggtctgtatt
    15781 atttgttgat gatcggcgaa gtgccgacca aggaacaggc gctcgaagtg gaagccgaat
    15841 gggccaagcg cgccaccgtt cctgactatg tgtataagat gctcaagacc atgccgaaag
    15901 agactcaccc gatgactctc ttctcgcagg ctgtccttgc gcttcagaac ggctcgaact
    15961 ttgcggccaa gtatcacagc atgaagaagg atgtttattg ggaagccgcc cttgaagaca
    16021 gcctcgacct gaccgcgaaa ctccccgtca tcgccgcata tatttaccgc atgaaatatt
    16081 atggcgagac ctccaagccg aaatacaacc ccaagctcga ttacggtgcg aatttctcca
    16141 agatgatgaa ggtttcagac aagaagggtt attccgaact tgcgcgcctg tatttcatct
    16201 tgcacagtga ccatgaggct gggaatgtct ccgcccatac catgcacctc gttgggtctg
    16261 ctctctccga cccgtacctt tccttctccg cctcgctcaa cggtcttgct ggacctttgc
    16321 atggtctggc caatcaagag tgcctcggct ggctgctgga agtacacaag aaattcggcg
    16381 gtgtgccttc ccgtgaggac ctgtacaaat tcgcctggga taccctcaac ggcggccatg
    16441 tcatccctgg gtatggtcat gcggtcttgc gcgttcccga tccgcgtttt atggcgcaga
    16501 tggaattcgc caagaagcgc ttccctgatg acgaattagt acgccttgcc gacctcgtct
    16561 tcgatgtggt gcctgccgtc ctcaaggaac agggcaaggc caagaacccc gccccgaatg
    16621 tggatgcgat cagcggtacc ttgcagtatt actacggtgt gcgtgaattt gatttctaca
    16681 ccgtgttgtt cggggttggc cgtgcgctcg gcgttactgc caactacgtt tgggctcgtg
    16741 cgctcggcat gccgatcgaa cgtcccaagt cgttgaccac caagatgctg gaagatgtgg
    16801 tcgcaaaagc cgcccaacaa cctgcatagt caatagaatc aatctcccga agtgaaaact
    16861 tcgggagatt tttatttaaa tcaggggtgt tgtgatggtt tatggttttg cgcccaacct
    16921 gtcgatcagg aacgcaaaat caagtacatt ttctttcaga tagtcatacc ttccactggc
    16981 gcctgcgtgg cctgcactga aatttgtttg aagcagggcg aggttgtcgt cgttcttttt
    17041 atcccgcagg cgtgcaagga atttggctgg ttcccaatag gccacccgtg gatcgttgaa
    17101 ccctgtggag agcagcaggt gcggatattc cacttcccgt agattgtcgt atggagaata
    17161 ggacagcatg tagtcgtatt catccctgtt cgcggggttg ccccattggt cgtattcgag
    17221 cgtggtcagc gggatgcttg ggtcactcat gctggtgatc acatctacaa atgggacttt
    17281 gcagatcact gcctcgaaaa gctctggccg catcaccatg cttgcgccga ctaggagtcc
    17341 acctgcggag ccgcctgtgc tggcgagctt ttcgggagag gtgaatcctt ctgcgatcaa
    17401 gtgctcggcg caggcgatga aatcggtgaa tgagttcttt ttgttgaaca tcctgccttc
    17461 gtcgtaccag aaacgtccca tttcggagcc gccgcgcaca tgagcaatgg caaagacaaa
    17521 cccacggtcg agcaggctca aaaggctgag gttgaatgcc gcgtcgctgg aggcgccata
    17581 ggctccatac ccatataaca aggttgggtt ttgaccgtct tttttcaatc cctttttgta
    17641 ggcaagggaa attggaatct tgcgcccatc cgctgcggtg gcatggatat attccgtgac
    17701 gtattgtgat ttgtcatagc cgcctgggat ttgatattcc tttttcacct cccactcgcc
    17761 gttatccatg tgaaagtcaa ccgtagagaa gggggtggtc aacgaggagt agcgcaggcg
    17821 taacaggttc gtgtggaatt cagggttgac atcaacaatg acctcgtaag tggggtcggg
    17881 gaagttgaca taacggacat cgctgtgacc atctggcctg ctgactctga gttgtttgag
    17941 tccgcctttt ctttcgtgaa ggacgatgtg ctccgcgaag acatccacgt gttcgagcag
    18001 ggtatcttca cggtgaccga tgacctcctg ccagttttcc ctgcttgggg cggcagtggg
    18061 tgttttcatc agcttgaaat tatgcgcgtc ttcattggtg acgatataga aatggtcatc
    18121 aaggtgggca gcgtaatatt ccagccctgc tttgcgtggt tgaaggacat tcagcgcccc
    18181 gacagggtgg tcggctggca ggaagcgcat ctcttgtgaa atcgtattgt aatgataggt
    18241 gaagatgtat ttttcactac gagatttgaa gagtgaaaga gagaaggtgt cgtcttcttc
    18301 gaagtacacc agcacgtcct gtgatggcgc cgtacccact ttgtgacggt agagtttaca
    18361 ggcacgatgc gattcatcca ggctgagata gaacactgtt tcgttgtcgt tggcccattc
    18421 cacgcctgcc tgaaaataaa cactgccatg gatattggca atggcttcgg ggtacattgt
    18481 gccgctgatg aggtctttga catgcagggt gtagacctca gagccttcaa ggtcaacgga
    18541 ataagccagc ttgttttggt ctgggctgac ggaaaaggcg ctgacactgc aaaattcatg
    18601 tccctcggcc agtgcgtttt gatccaacag gatttcctcg gctgcattgg ggatattttt
    18661 tttacgacaa aagatggggt attgtttctc tgcttctgtg cgggtgtagt acaggaagtc
    18721 accatgtttt tcggggacac tgtggtccac ctcgcggatg cgcgctttca tttcgtgaaa
    18781 gagcttttca cgcagcggat tcaggtgttg aagagtttct tcgaggtagt cattttcggc
    18841 ttgcagatag gctttgacct ctgggtcttc acggttgcgc atccagtagt attcgtcgtt
    18901 gcgggtctgt ccatgctggg taagggcatg gtctcgtttg ggtgcaattg gcggcatgat
    18961 gctcctgttt gggttaacgg taatcctgtg agatttcttc taaattgatc aggttgctgt
    19021 cgattcggat gtagattccg ttttcgagtt tttggtattc ttcttttccg cgcataaaat
    19081 cggcgagggg ggcgaagtta tctgcgatca ggtcgggacg aaggtggatg atgctgtagt
    19141 catagtccca tttgttgtga cctggataga actcgccgtg gccctggcct ttggccacaa
    19201 tggggtcgtt gagtccgagc aggtcgatgg tggtgcgttg cgagtagtat ggaatctgcc
    19261 cagcggcgtg gacggcgatc acggcttcgg gtgaggtgga ttcagcgagg aagagtccca
    19321 gccgtacgcg gcggatatcc gctttgagga ggggggcgtt gtcaacggtg taggtgaacc
    19381 atggctcgcc gctgacgatg agcagggtga tgaccgcaag tggaatggca ggattcactt
    19441 tcggggcggt gaatgctgat tggggctggg ccgcttgcag gtcggtgagg atgcggctcg
    19501 tcatccaact gaaaaggatg atgagggcgg gcattccctg ggtgatgaag cggttggcgg
    19561 cgttcgtttc aggctctgca taatcaccgc caacatagat ggagtaggca gattgagcga
    19621 ggaagatgcc gagcagtaaa agtgtttcgc ggctgcgttg cagtttatag aagaagatgc
    19681 ctgccagtga aaacaaaaag agcggaagtg tatcgcgtgc ggcgaattcg acgaagacca
    19741 gcagcccgtg acggatgcgt tcgagggcgg tgaaccctgt cattttttgg taataggtgg
    19801 cgggaagaaa atcgccgaag taattgtgtt gaaaatagaa aacggccagc aatgtgatgg
    19861 cggctgatgc gatggggagg atggcattgc gtttgttttt gagcagcaga taaagcgcga
    19921 tgggtgcaat ggaaatgatc gcgtccacgc ggacgagaat cgccgctgca aatatgaggc
    19981 cgataaaaac ggggtgtttc tcggtgatcg agaaaagaac tgcaagactg acgagcaagg
    20041 caagcaagcc cacttccatg ccacgcagtg accagaacac cagcggaaaa taaaatgccg
    20101 tgatgagggc tgccagtgtg ggcgcgtatt ttgaatcggg ttggagcgct tctgcaattt
    20161 tggaaactgt gtagctgttt gcgagcaaaa tcgccagcga ggtgagcatg accacaaggg
    20221 agattttcga ggctggaaac gggaagagat gcagaaaagc gagatagagc gtccagccca
    20281 ggttggtgaa gccttccaca ggcgcttcgc cgatattcca taccaacccg tgaccttgtg
    20341 cgagattgcg ggcgtagcgc atggagatca tggcgtcatc tacgagggtg aaatagcgct
    20401 cgcctttgat atcgaaggag gtgccgatga tgaaaatgag atagaagacg gcagaagtga
    20461 taagaatgat tttgagcggg gaatgctttt ccatgagttg tgggattata tcgcacggga
    20521 gggaaagcaa aaggctgacg aaacatcgcc agcctt
//
LOCUS       DAOQLC010000078        20052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_938_length_20052_cov_24.143722, whole genome shotgun sequence.
ACCESSION   DAOQLC010000078 DAOQLC010000000
VERSION     DAOQLC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 20052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_938_length_20052_cov_24.143722"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PL000_13780"
     CDS             complement(<1..682)
                     /locus_tag="PL000_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90020.1"
                     /translation="METSKITSEHEHPSLALANILFATWLYSLLWGGGIFFSALAFFG
                     AIVAGLLSFFGPPIWNATMIIALILGLAATYTSSIFLWRIITHDQQQSANIGFISFLA
                     STSIFLLTWIGIGAFSEWAHHNWSSYFGNSFLFLRTIDDIGPILIIGFTTCATIVSGL
                     IKTYHYQYRELIASAVIGIGISIIDGIILEKTLLKNNEIFHLTWQIPSLVWVSVVYFA
                     ELLAGRSKW"
     gene            844..1623
                     /locus_tag="PL000_13785"
     CDS             844..1623
                     /locus_tag="PL000_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNC90021.1"
                     /translation="MHERLSFHIKRDSVIHKLNPLTKLTLILALILISFTSPWYWTPH
                     VVLLLAVIPLSGLGRVFPEFFKAATRLILPAAGFLFLMQAFFQPIGEEVIFKFYFLDI
                     TQESLMFAFRNAMRVFVMISAFTLFLLTTHPSELMSDLTRRGLPGQFAYVIISTLQIL
                     PQMQAKAQTIIAAQRSRGLDTESTFLKRAGSVLPLVGPLVFGSLVEVEERAIAIEARG
                     FTSKNSKTSLHEISDPSFEKTIRWALLMLVILSIAANLWLS"
     gene            1611..2447
                     /locus_tag="PL000_13790"
     CDS             1611..2447
                     /locus_tag="PL000_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC90022.1"
                     /translation="MAFVNLQNVTYQYPLTKTPALQNVNLQVNEGEFVAVVGPNGAGK
                     STLCYTLAGFAPHFFKGEMSGTVEVAGKESSKSTLDEWVLNVGLAFQNPFNQISGAKY
                     TVFEEIAFGLENIGVPRDEMKNRVEDAMALTGIRDLADRSPYSLSGGQQQRVALTSIL
                     VMRPKLLVLDEPTSQMDPIGTREVFGTIRKMAEEGMTVVLVEHKVEWIAEFADRVVAL
                     KDGSVLLEGKPNEVLTSELLAEHGFGLSRYTSTAREARKRGLWKKENLPVTLDEAVEG
                     FS"
     gene            2483..3274
                     /locus_tag="PL000_13795"
     CDS             2483..3274
                     /locus_tag="PL000_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC90023.1"
                     /translation="MKIEIKDLRFIYPTGLEALKGISLAIESGEQVAIVGQNGAGKTT
                     LVRHFNGLLQPASGSVLIGDWDVKKHSVAKLASRVGYVFQNPDEQLFSRDVLTEVTFG
                     PKNLGYAREKIDQLVKRALALTELNDKTETNPYDLSPTWRKMVALASIIAMDTPIVIF
                     DEPTTGQDAVNVARIANVIAELRKEGKTVITITHDIDFCAENFERVIALSKGQVLLDG
                     PAREILGQEEILAQTYVDPPQLTRLGKRLGLKETVRNQEEFLNSL"
     gene            complement(3337..3735)
                     /locus_tag="PL000_13800"
     CDS             complement(3337..3735)
                     /locus_tag="PL000_13800"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC90024.1"
                     /translation="MKAHTFRLKPAQDLFDSIEEFVKQNNIQAGCVLSSVGSLTHATL
                     RLANRSHYNEYEGHFEIVSMTGTVSIHGSHIHVAISDGDGVTIGGHLVSGCKIYTTAE
                     IVIAEFEDVVYKRELFENDSGYEELVVYKT"
     gene            3762..4472
                     /locus_tag="PL000_13805"
     CDS             3762..4472
                     /locus_tag="PL000_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNC90025.1"
                     /translation="MKSYRYFDIILGVFIAVLLISNVASSAKIVDFGFGIFGIPLAFD
                     AGTILFPIGYVFGDILTEVYGYQRSRRVIWTGFFSLALAAFVFWIVGRLPGEVTWQGY
                     AGDAAYQSILGGMSSGGILFASLAGFWLGEFSNSFILAKMKVWTNGRWLWMRTIGSTL
                     VGEMMDTAIFVVVATAFGVFPWSLFLTLTVTNYLFKVMVEVVMTPLTYIVVHALKRAE
                     NEDYYDRETNFNPFAIIK"
     gene            complement(4502..5062)
                     /locus_tag="PL000_13810"
     CDS             complement(4502..5062)
                     /locus_tag="PL000_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNC90026.1"
                     /translation="MSFRSLLELDARLSGQMRVAEKPGFLRNVAAFFAHSGDSWFWFA
                     ALLLFWFFSNSFWKHWEVVEFFGILGLAGVVLGIKFLVRRKRPEGEWGGIYRNTDPHS
                     FPSGHAARSFLIAVLASFLAPPWLAAVLWVWAPLVALARVAMGVHYLSDVIAGAILGV
                     IVALIGMQIYQPMIDWFTALTHFSFW"
     gene            complement(5059..5967)
                     /locus_tag="PL000_13815"
     CDS             complement(5059..5967)
                     /locus_tag="PL000_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC90027.1"
                     /translation="MDAIVTAGGIPQPGDPLYVYSNGNSKALIDISGKPMIQWVLDAL
                     GESKKVDNVIVIGLSPKSGLTCKKPLHYISNQGRMLANIVAGVNKSLELNKKNEYVLI
                     VSSDIPALKGDMVDWLVDTCMSTKDDLYYGVCPREVMEERYPTSKRTYTKLKEMELCG
                     ADINVIHVSMATDHLNTWEQLIGNRKSPLRQAAVIGWDTLFQLATRQFTLQGLVERAS
                     QRIGIKGRAIVWDKAEPCMDVDKPHQLELMREDIASQLQKAAKAEKKKVAAAKKAAEP
                     VSKTKKTTASAAAKPKPKPKAKVSTK"
     gene            complement(5970..6875)
                     /locus_tag="PL000_13820"
     CDS             complement(5970..6875)
                     /locus_tag="PL000_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90028.1"
                     /translation="MKRAVSISIGSSKRNKAVEVTLLGEKVSIERIGTDGDMEAAALK
                     YQELDGTVDAFGVGGADLGAIVEGKFYPFHSVQKLIRYIKKTPVVDGGGLKNTLENKA
                     PGFLDKKIGDYINQRGRKVMVTVGVDRWGLSKSFAEANYETVFCDLMFALDVPIPVRT
                     MKGLRTLAAILIPIVTRFPFEWLYPTGDKQNEHKPKYENWYQWATVIAGDCLYIKRHM
                     PLNMQGKVVVTNTTTPEDVEFFRKCGIKYLITTTPVMDGRSFGTNMMEAALVAISGKG
                     RPLSFDELNELLDKLGFEPQLQELN"
     gene            complement(6954..9551)
                     /locus_tag="PL000_13825"
     CDS             complement(6954..9551)
                     /locus_tag="PL000_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90029.1"
                     /translation="MPNRFRRLLSSPIFENEEKTRTAQILNSFVWSAISILLIIIIIR
                     AIIGQEENPIPLLVLTAIVVALGIVQVIIRRGYIRGASIFLAGSFWVIITFQAWAADG
                     IRDAAVISYILIILLSALILGWREGLFFGVISLSALWFFAVEEEWGLRVQHMDTPLNL
                     ARDFTVIFLFAGTLIYLLINSLNRTLYDARLELKERLRAERKLQVQADYLTALNETAL
                     GLLNRSELRPLLESILSRACALLDTQHGLIDLVLPDESALRQELGHGVLSRYDGSLTQ
                     KNVGVTGEVWGSGKTLVIQNYGEWEKNLPEFVDAGFNAVMGVPLKVGNKVIGVLAISF
                     VEQERTFTHDHVNLMERFAALASLAIDNARLFEQTQKEIAERTMAEAALRSSEERFRK
                     VFHASPVAIVITSLSEGTIIEANEAYWRLSGLDLQKTIGYQATELGLWQDEEEPGDFI
                     ERLKAERSIYVPEYLFLNEREGLYSTVLAFYELVKLEGTDSILSMFHDITEQKNARES
                     LQNSEARTRAILASIPDMIFEISKDGVFLDFMASSQLVPLMPPSQFLGRTVQELFPPI
                     IAQQTLFALERTLATGHLHAFEYGMPPGEEVQFFEARITAISPESAIIMVRDISQQKY
                     VTMEREKLINELEDKNSELERFTYTVSHDLKSPLITIKGFLGFLEQDALSGNTTRLKL
                     DIQRIGDATDKMQLLLSELLELSRIGRLTSPYHFIKVEDLVTEAVEALHGRLNKSHVK
                     VETQGDLPTIYGDHQRLLEVFQNLIDNAAKFTEGQPDPHIEIGQAGLENEQPIIYIRD
                     NGIGIDPIHHDRIFGLFNKLDASSEGTGIGLALVKRIIEVHGGRIWVQSEAGHGSTFF
                     FTFPNGPES"
     gene            9792..9885
                     /locus_tag="PL000_13830"
     tRNA            9792..9885
                     /locus_tag="PL000_13830"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9826..9828,aa:Sec,seq:tca)
     gene            9968..11146
                     /locus_tag="PL000_13835"
     CDS             9968..11146
                     /locus_tag="PL000_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNC90030.1"
                     /translation="MSIPETEILTNLVSRVFRIEDVTSEDPRKGFFLRYRGVLANEDS
                     AALYDTLADSLSPYGIIPLFRMEEGRQVVYLAPKPPEPKQDRISTNILLFVLTVFSVM
                     LAGAQPEGTMPDDFMGQLLMLAKSIFTGWPFALSLLGILLTHELGHYFMSRYHKTPAT
                     LPYFIPLPLSLLGTMGAAIIMRGTPKNKRVLFDIGVAGPIAGLIVALPVLFYGLSLST
                     LGTIDPNPNGFIEGNSLMYLLAKYITFGQLLPSPLEPQGILYWLQYFFTGRPVPFGGL
                     DVFIHPVAFAGWAGLLVTGLNLIPAGTLDGGHVIYGLFGEKARKGFPFIIGILAILGI
                     FWSGWWLWALLLFWLGRVNAEPLDQITTLDPPRRLLGYIMILVFLLVFMPVPFMTLAQ
                     "
     gene            11154..12530
                     /locus_tag="PL000_13840"
     CDS             11154..12530
                     /locus_tag="PL000_13840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC90031.1"
                     /translation="MKKIILSFIIFTFVLAACGTQEDVTSTAEPAVTQAATKTPQPEP
                     TVEAASRLGVGEEALNGLEITVWHPWYGVEASLFESFVEDFNESNSWGIKVVAESQVS
                     FTNLYENLMASIPGKDRPDLAIALPEHALEWDNNGMSVDLMPYVNDPAYGLDSSNIPV
                     VFWNQDLDGEKRVALPAQRTARFLLWNETWADEIGFASPPDAPDDFRQQACRAQQFML
                     KDESPQNDFKGGWYADTDYMTAYAWLLAFEGGVLEGSDYRFLTPNNIEAFKFLRELSE
                     STCSWQSADTDAVTAFANREALFITASLEDLPDAARAFASAGNTDTWKVLSFPGENED
                     ALVVYGSSYVMLKSTNEEQLAAWLFARWLTDEKQDARWVEATHLFPLRTTTLDLLADY
                     KKTHPQWEQAVKLLPQGEIQPQLDSWRKVKIMLGDGFTHMYRVNVPSGQVAAILAQME
                     ALSRELSR"
     gene            12737..13528
                     /locus_tag="PL000_13845"
     CDS             12737..13528
                     /locus_tag="PL000_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90032.1"
                     /translation="MPESEYRARQVRAKTNEVFEYDRDDEKPGKPLHILIPGGLIALA
                     TLALIVFIAYQTWFEGNLAENIGLILVVILSPFYVGGVFLFSYGYELYNIPRALRLTA
                     IIVFVTLAAVVIVAVLFFLLANMNGSSGSKSSSSNSSKGGKSRSSSSSSSSKPSNSPR
                     PSTGKMGGIVGAAGAALNRSSTNSSSSDSSSSSGYSSGGHVYIGGGETREVTKEVVKE
                     VPKEPMPVTCPFCARSYVPVENNYICPSCGAATPRELIKESQEPK"
     assembly_gap    13530..13629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13765..14046
                     /locus_tag="PL000_13850"
     CDS             13765..14046
                     /locus_tag="PL000_13850"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNC90033.1"
                     /translation="MKSSEFEWDDEKAERNFKKHGVGFEEGATIFNDPMIATIDDPDH
                     SENEARFISIGMSVVRRLLTVIHTFRKERIRLISARKATKNEKKYYKSH"
     gene            14018..14353
                     /locus_tag="PL000_13855"
     CDS             14018..14353
                     /locus_tag="PL000_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90034.1"
                     /translation="MKKNTTKAIKKTKNNDGRSEYDFSKAQRGRFYRPLDKGYTVHVR
                     KSDGTEVVNHYTLKEGTVLLAPDVLEYFPDSESVNEALRSLIHLMENVPVKKYSVSKM
                     ATHQVAERK"
     gene            14430..15893
                     /locus_tag="PL000_13860"
     CDS             14430..15893
                     /locus_tag="PL000_13860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC90035.1"
                     /translation="MRQMLINGKFTNGNATEEIEVQNPATEEVIESVPRGTPQDVEDA
                     VQAAKSAFDSWRKLGSNERARLLHEVADRVHEHREKIVHMLTLEEGKPVSENEEEVEW
                     VINTFHYYAELGRHHRGSVLASGEASQFNFIIKEPYGVVGCIVPWNYPLLLLAWKVAP
                     ALAAGNTVVIKPSEMTPLTALYLAKHCFNGLPAGVVNVVTGYGLETGEPLVKHPDVPV
                     IAFTGSLATGQRIASIAAPMMKKLHLELGGKDATVIAEDADPEIAAKAVAYAALINAG
                     QVCTSTERVYLPKTHAAKFTEAIVEHVKSLKLGPGLDPKTDMGPMIGETFRAKFEKQL
                     VDARASGAKILTGGARPQYLEKGFFHEPTVLTGVNHSMVIMREETFGPAVPIMEYASF
                     DEAIQLTNDCQYGLGAVLISNDPKKIKRFFDDVKAGTIWINDPLTDNYAGPFGGMKYS
                     GGARELGEEGLEEFRETKHVHWDFNMEEKGYWFPYGR"
     gene            15944..17128
                     /locus_tag="PL000_13865"
     CDS             15944..17128
                     /locus_tag="PL000_13865"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNC90036.1"
                     /translation="MRKQSWLFPFVLLLAVILYFQISRVQLPGDGFSTFGSDTVRAEV
                     IQIIEEGEVDLGGHTQKYQIARVNILEGEYEGIPMEIDYGKRQLRSDDYDLVVGGRVM
                     VSISKTPDNVVNAYFVDFVRTSPLLWLTVIFAAAIILISRWKGVRALLSMAFSLYVII
                     DQIIPHILNGDDPLWVSIIGSIVLLSVTLYLTYGWNLKTHASVLSMVLVLLLTGALSA
                     VFVIFTKLNGTGDENVMFLMQMMEKPLNLRGLLLGGMIIGALGVLDDLVTTQSAAVFE
                     LHHANPSFKFRDLYNSAMRIGQDHVAATVNTLVLAYAGASLPMLLMFSLGHGNYGYII
                     NFSFIAEEVVRTLVGSLGLIAAVPLTTAIAILFAQRAELLGRWEQVLGPEGSGDGHGH
                     AH"
     gene            17242..17844
                     /locus_tag="PL000_13870"
     CDS             17242..17844
                     /locus_tag="PL000_13870"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC90037.1"
                     /translation="MNEEQTWVIQAQQGSDEAFTHLVETYQTPVFNLCYRMLGEPDLA
                     EDAAQETFLRAFQHLHRYDQKRSFATWLLSIAAHYCIDRLRRRKFTIFSMDTEDEDGN
                     TFELPDLDSPNPEAESIKGQTRDRIQAMLKDLDPTDRAAIVMRYWYDYSEVEIADSLR
                     LTVSAVKSRLHRARKELAGLWQEEETRAETERRPYESPAF"
     gene            17825..18355
                     /locus_tag="PL000_13875"
     CDS             17825..18355
                     /locus_tag="PL000_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90038.1"
                     /translation="MNHQPFEEWLLNDKNLNSAEKRELDLHLRTCAHCTALSATGLAL
                     RSANVVAPASGFALRFQQRLAAQRIAERRRKLWGAMVLVLSGFALLTWFVAPFVYAFL
                     NSPAEWITAAVSYFLFFASSVQALTEAAAVLFRILPDFVPPYMWMVFVSALAGLGLLW
                     TISIWQFSRTPQGVSA"
     gene            18352..19437
                     /locus_tag="PL000_13880"
     CDS             18352..19437
                     /locus_tag="PL000_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNC90039.1"
                     /translation="MRSKLTTLFLLLALLFLPTGAVHAQSPDGGDVFLLGQNYTLKSD
                     ETLDGSLVVIGGNVDIEKDATVNGDSVLIGGNLQVNGDANGSVVLIGGNLVISGKIKG
                     DIVLIGGQALLTETAVVDGDIVTMGGQVQKDPGAEVTGQIVNNAPPIDVPDIPDVPNV
                     PDAPDAPDAPDIPDVPGVPQINVDFNPLWEVTGVFTRALAIAAVGMLLTLFLQPQLDR
                     VADAITRQPMMAGSFGLLTVVVAPVAILIMIVTILLIPVAAIVAFILLPMAWLFGMVA
                     IGQEIGDRFTKAINQTWAPVLSTGFGTFLFLLVGGFIGLIPCIGWLISLMFSLVALGG
                     VMMTWFGTRAAPGSAPAVVTETIPPAS"
     gene            19658..>20052
                     /locus_tag="PL000_13885"
     CDS             19658..>20052
                     /locus_tag="PL000_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90040.1"
                     /translation="MFTKQEIPNTPIHNTPPPLIEVSGTHREMGRQIGEAARAQVQHS
                     VANARVLIDAAYDSLELTWQGAQIQSRKYLPFAEERYPQYVDEMRGIAEGANVAFDDI
                     VTLNAMEAVTVDALHLTRCTSMAVNEERTA"
BASE COUNT         4689 a         4940 c         5307 g         5016 t
ORIGIN      
        1 tccatttact ccgccctgcc agtagttcgg caaagtaaac aacacttacc caaacaaggg
       61 atggaatttg ccaggtaagg tggaatattt cattgttctt aagtagggtt ttctctaata
      121 ttattccgtc aattatggag atgcctatgc caattactgc actagcaatc aactcacgat
      181 attgatagtg gtacgttttt attaaaccgc taacaatcgt agcgcaagtt gtaaaaccta
      241 tgatcaatat tgggccaatg tcatcaatag tacgtaaaaa tagaaacgag ttcccaaaat
      301 aacttgacca attatgatgc gcccattcag aaaaagcacc tatgcctatc catgttaata
      361 aaaagattga tgtgcttgct agaaacgaga taaatccaat attagcactt tgttgttgat
      421 catgtgtgat gattctccaa agaaaaattg aactggtata tgtggctgct aatcctagaa
      481 tcagggcaat aatcatcgtg gcgttccaga ttggcggacc aaagaaactt aataaacccg
      541 ctactattgc cccaaagaag gctagtgcgg agaagaatat gccccctccc caaagcaggc
      601 tatataacca tgtggcaaac aatatatttg ctaatgcaag agagggatgt tcatgctcag
      661 atgtaatctt actagtttcc attggtctgt tccttttcgg gaggacattt tctctttccc
      721 cccaaggttt cttcaaaatc aattatacgg ggaatcgttt tgaggtgaag tgacatgtgt
      781 cacagggtat ggatggtttc tgggtagcgg tcacacccga tttttgtcgt ttataattct
      841 cccatgcatg aaagactttc cttccacatc aaaagggaca gcgtcattca caaattgaat
      901 ccgctgacga agttgacctt gatccttgcg ttgatcctga tctcgttcac cagtccctgg
      961 tattggactc cgcatgtggt gttgctcctg gccgtcattc cgctaagcgg attgggcagg
     1021 gtcttccccg aatttttcaa agccgccacg cgtctgatcc ttcccgcggc tggctttctt
     1081 tttttgatgc aggccttctt tcagcccatc ggtgaagaag tcatctttaa gttctatttc
     1141 ctcgatatca cacaggaaag cctgatgttt gcgtttcgga acgccatgcg ggtctttgtg
     1201 atgatctcgg cattcacctt gttcttgctg accacgcatc caagcgagtt gatgtctgat
     1261 ctcacgcggc gcggattgcc ggggcaattt gcctacgtca tcatttccac gctgcagatc
     1321 cttccgcaaa tgcaggccaa agcgcagacc atcatcgccg cccagcgttc acgtgggctg
     1381 gataccgaaa gcacctttct caaacgcgct ggttcggttc tgccgctcgt gggtccgctg
     1441 gtgttcggtt cactggttga agtggaagaa cgcgccatcg ccatcgaagc gcgcggcttt
     1501 acctccaaaa acagcaagac ctccctgcac gaaatttccg acccatcgtt cgagaaaacg
     1561 attcgatggg cgcttctcat gctcgtcatt ctttccattg cggcaaacct atggctttcg
     1621 taaacctcca aaacgtcacc tatcaatatc cgctcacgaa aacgcccgcg ttgcaaaatg
     1681 tcaacttgca ggtgaacgaa ggcgaattcg tcgcggtcgt tgggccgaat ggcgcgggca
     1741 agtccacgct ttgttatacg ctggcaggtt ttgcgccgca ttttttcaaa ggcgaaatga
     1801 gcggcaccgt ggaagtggct ggcaaagaat ccagcaagtc cacgcttgat gagtgggtct
     1861 tgaatgtcgg cctcgcgttt cagaatccct tcaaccaaat ttcgggcgcg aaatacactg
     1921 tctttgaaga aatcgcattc ggtctcgaaa acatcggcgt gccgcgcgat gaaatgaaga
     1981 atcgtgtgga ggatgccatg gccctgaccg gtatccgcga tttggcagat cgctcgcctt
     2041 attcactctc cggcggacaa caacaacgcg tcgctctgac ctcgatcctg gtcatgcggc
     2101 ccaagctgct cgtcctcgac gaaccgacct cgcaaatgga cccgatcggt acccgcgaag
     2161 tcttcggtac gatccgcaaa atggccgaag aaggcatgac cgtcgtcctc gtggagcaca
     2221 aagtggaatg gatcgcagaa ttcgcagacc gagtcgttgc gctcaaagat ggcagtgtgc
     2281 tgctcgaagg caaaccgaac gaagtgctca cctctgaatt gctcgccgaa catggtttcg
     2341 ggctttcacg ctacacgtcc acggcgcgtg aggccagaaa acgcggactt tggaaaaaag
     2401 aaaatctgcc cgtcacattg gatgaggctg tggaagggtt ttcgtaaggg cacagcgccg
     2461 ctgtgcccca acgggataat caatgaaaat cgaaatcaaa gaccttcgtt ttatctaccc
     2521 cacaggcctc gaagccttga aaggcatttc actcgccatt gaatcgggtg agcaggttgc
     2581 cattgtcggt caaaatggcg cggggaaaac caccctcgtg agacacttca acgggttgtt
     2641 gcaaccagct tccgggtctg ttttgatcgg ggattgggat gtgaaaaaac attcggtggc
     2701 gaaactcgcg tcgcgagtcg ggtacgtgtt tcaaaatccc gatgagcaat tgttttcacg
     2761 cgatgttctc accgaggtca cgtttgggcc gaagaatttg gggtatgcca gagagaagat
     2821 cgatcaactg gtgaagcggg cgctggcatt gaccgagttg aatgacaaaa ccgaaaccaa
     2881 tccctacgac ctttcgccga cgtggcgcaa gatggtggcg ctggcttcga tcatcgcaat
     2941 ggatacgccc atcgtcattt tcgatgaacc cacgacaggg caggatgccg tcaacgtggc
     3001 gcggatcgcg aatgtgatcg ccgagttgcg caaggaaggc aagaccgtca tcaccattac
     3061 gcacgatatt gatttttgcg ccgagaattt tgagcgtgtc atcgcgcttt caaagggaca
     3121 ggtgctgctg gatggccccg cccgcgaaat attggggcag gaggaaatcc tcgcgcagac
     3181 gtacgttgac ccgccgcagt tgacgaggtt gggcaaaagg ctggggttga aagagaccgt
     3241 gagaaatcag gaagaatttt taaattcatt gtaaggtgta ggggcgcagc gccgctgcgc
     3301 cctttttcgt tatagggaca cggcaacgct gtgtccctac gttttataca ccaccaattc
     3361 ttcatatccc gaatcgtttt caaataattc ccgcttgtaa accacatctt caaattcagc
     3421 gatgacgatc tcagccgtgg tgtaaatttt gcacccgctg acgagatgcc cgccgatggt
     3481 gacaccgtca ccatccgaga tcgccacgtg gatgtgtgaa ccgtggatcg aaacagtgcc
     3541 tgtcatggaa acgatctcga aatggccttc atactcgttg taatggctgc gattcgccag
     3601 ccgcaaagtg gcatgtgtga ggctccccac agacgaaagc acacaacctg cctgtatatt
     3661 attttgtttg acgaactctt cgatcgagtc aaataaatcc tgtgcgggtt tgaggcggaa
     3721 ggtatgggct ttcataaagg ctccttggta aaatcgcgga tatgaaatct tatcgttatt
     3781 tcgacatcat tcttggtgtg ttcatcgctg tgctgttgat cagcaatgtc gcatcttcag
     3841 ccaagatcgt ggattttggt ttcggcatct tcggtattcc gctggcgttc gatgcgggga
     3901 caattctttt cccgatcggg tatgtcttcg gcgatattct aaccgaggtc tatggctatc
     3961 aacggtcgcg gcgcgtgata tggacagggt tcttttcgct ggcgttggcg gcgttcgtgt
     4021 tttggatcgt gggacgtttg ccgggggaag tcacctggca gggctatgca ggtgatgcgg
     4081 cgtatcagtc cattcttggc gggatgagca gcggcggtat tttgtttgcc agccttgcgg
     4141 ggttttggct gggtgagttc agcaattcct tcatcctcgc aaagatgaaa gtgtggacga
     4201 acggccgctg gctatggatg cgtaccatcg gaagcacatt ggtgggcgag atgatggaca
     4261 cagcgatatt cgtcgtggtg gcgactgctt tcggtgtttt tccgtggagc cttttcctca
     4321 cactgacggt gacgaattat cttttcaaag tgatggttga agtggtgatg accccgctga
     4381 cctatatcgt tgtccacgct ttgaagcggg cagaaaatga agattattac gaccgtgaga
     4441 cgaatttcaa tccgtttgca ataataaaat agcgcgggat attatcccgc gctacgtgtg
     4501 attaccagaa tgaaaaatgt gtcagtgcag tgaaccagtc gatcatgggc tggtagatct
     4561 gcatcccgat cagtgccacg atcacgccta aaattgcgcc tgcgatgaca tcagaaagat
     4621 agtgcacgcc catggccaca cgtgccaaag ccaccagcgg cgcccacacc caaagaacag
     4681 cagcaagcca gggcggggca agaaacgagg caaggacggc gatcaaaaac gaacgggcgg
     4741 catgtcccga ggggaaggag tgcgggtcgg tgttgcggta gattccgccc cattcgcctt
     4801 ccggtcgctt gcggcggacg aggaacttga ttccaagcac aacacctgcc agcccaagga
     4861 tgccgaagaa ttcaacgact tcccagtgtt tccaaaacga attgctgaaa aaccaaaaaa
     4921 gcagaagcgc tgcgaaccaa aaccacgagt cgccggagtg ggcaaaaaag gcggctacat
     4981 tccgcaaaaa acccggcttt tcagccacac gcatttggcc ggagagtcgg gcatcgagtt
     5041 caagaagaga ccgaaaactc atttggtgct gacctttgcc tttggttttg gtttgggctt
     5101 tgcagcagca gaagcggtgg tctttttcgt tttgctgaca ggttcggcgg cttttttggc
     5161 ggcggcgact ttcttcttct ctgccttggc ggccttttgt aattgactgg cgatgtcttc
     5221 acgcatcagt tcaagttggt ggggcttgtc cacatccatg cagggttcgg ccttgtccca
     5281 tacgatggcg cggcctttga tgccgatgcg ctgggaggcg cgttcgacca gcccttgcag
     5341 ggtgaattgg cgtgtggcta gttgaaagag agtatcccaa ccgatgacgg cggcctgacg
     5401 cagggggctc ttgcggttgc cgatgagttg ttcccaggta ttaaggtgat cggtggccat
     5461 gctgacgtga atcacgttga tatcggcgcc gcacagttcc atttctttca gtttggtgta
     5521 ggtgcgtttc gatgtggggt agcgttcttc catcacttca cgcggacaga cgccatagta
     5581 caggtcatct ttggtgctca tgcaggtatc caccagccag tccaccatat cgcctttcag
     5641 ggcggggatg tctgaggaaa cgatcaacac gtattcattc tttttattta attcaagcga
     5701 cttgttgacc ccagcaacga tattggcgag catgcgtccc tgattggaga tgtagtgcag
     5761 gggcttcttg caggtcaatc cgcttttggg cgagaggccg atgacgatga cattgtccac
     5821 ttttttcgat tcgcccaacg catccaaaac ccactggatc atgggcttgc ccgaaatatc
     5881 gatcagcgct tttgaattgc cgttggaata tacatataaa gggtcaccgg gttgtggaat
     5941 tccaccggca gttacgattg catccataat taattcaact cctgcagttg gggttcgaaa
     6001 ccgagtttgt cgagcagttc gttcagttca tcgaaggaaa gcggacggcc tttgccggag
     6061 atcgccacca gcgcagcttc catcatgttc gtgccgaagg aacgcccatc catgaccggg
     6121 gtggtggtga tgaggtactt gatgccgcac ttgcggaaga attcaacatc ttccggggtg
     6181 gtggtgttcg tcactacaac tttgccctgc atgttcaatg gcatgtggcg tttgatgtaa
     6241 agacaatcac ccgcgatgac ggtggcccac tgataccagt tttcatactt gggtttgtgt
     6301 tcgttctgtt tatcgccggt ggggtacagc cattcgaagg ggaagcgtgt cacgatcggg
     6361 atgagaatgg ccgcgagggt gcgcagtccc ttcatggtgc ggacggggat gggcacatcg
     6421 agtgcgaaca tcaggtcgca gaaaaccgtt tcatagttgg cttcggcaaa ggatttggag
     6481 aggccccagc gatccacacc gacggttacc atgaccttgc gtccgcgctg gttgatgtaa
     6541 tcgccgatct ttttgtcgag aaagccgggg gctttgtttt caagcgtgtt tttcaacccg
     6601 ccgccatcca ccacgggggt ctttttgatg tagcgaatca atttttgaac ggaatggaat
     6661 ggatagaact tgccttcaac gatcgccccc agatctgcac caccgacacc aaaggcgtcc
     6721 actgtgccgt ccaattcctg atatttcagg gcggcggctt ccatatcccc atcggttcca
     6781 atgcgttcga tgctgacctt ttcaccaagc aacgtcactt cgacggcctt gttgcgcttt
     6841 gatgaaccga tgctgatact cacggctcgt ttcatatatt tagtctcctc gtttgatggg
     6901 ttaaagcgag tataccccga agtcaggtgc gtgagaatga cattgaaaca ggatcaggat
     6961 tcaggtccgt tgggaaacgt gaagaagaaa gtcgagccgt gccccgcttc gctctggacc
     7021 cagatcctgc ctccatgaac ctcgatgatt ctcttaacca gggcaagacc aatgccagtg
     7081 ccttctgaag aggcatccag tttgttgaac aggccaaaga ttcggtcatg gtgaattggg
     7141 tcaatgccga tgccattatc acggatgtaa atgatcggtt gctcattttc cagcccggcc
     7201 tgcccgatct cgatgtgagg gtcgggttgt ccttcggtga atttggcggc attatcaata
     7261 aggttttgaa atacttcaag caggcgttga tggtcgccat aaatcgtcgg caggtcgccc
     7321 tgggtttcca ctttgacatg ggacttgttc aatcgtccat gcagggcttc cacagcttcg
     7381 gtgacaaggt cttcaacctt gatgaaatga tatgggctgg tgagccgacc aatgcgggaa
     7441 agttcaagca attcactgag cagcaattgc attttgtcgg tggcatcacc gatccgttga
     7501 atatcaagtt tcaggcgggt tgtgtttccg ctgagtgcat cctgctccag aaaaccgagg
     7561 aagcccttga tggtaatgag aggtgatttg aggtcgtgcg aaacagtgta ggtgaagcgt
     7621 tccagttcgc tgttcttgtc ttcgagctcg ttaatgagct tttcgcgttc catggtcaca
     7681 tatttttgct ggctgatgtc gcgcaccatg atgatggcag attcagggct gatggcggtg
     7741 atgcgtgcct caaagaattg cacttcctcg ccaggcggca ttccatattc gaaggcatgc
     7801 agatgccctg tagccagggt gcgctcaagt gcgaacaggg tttgctgggc aatgatgggt
     7861 ggaaatagtt cctgcaccgt cctgcctaaa aactgtgatg gcggcatgag aggcacaagc
     7921 tgggaggaag ccataaaatc gagaaatacg ccgtcctttg aaatttcgaa gatcatatcc
     7981 ggtatggagg caaggatggc gcgggtgcgc gcttcgctgt tttgcaggga ttcgcgcgca
     8041 tttttttgct cggtaatatc atggaacatg gaaaggatgc tgtctgtgcc ttcgagtttc
     8101 accagttcat aaaaggccag tactgtgctg tagagtcctt ccctctcgtt caggaataaa
     8161 tactctggaa catagatgga gcgttcggct ttcaagcgtt caataaaatc accgggttcc
     8221 tcttcatcct gccatagacc caattctgtg gcctgatacc caatggtttt ttgcaggtca
     8281 agcccggata acctccaata cgcctcattg gcttcgatga tggtgccttc actcaaagaa
     8341 gtgatgacaa tggccaccgg gctggcatgg aataccttgc ggaagcgttc ttcgcttgag
     8401 cgcagggcag cttcagccat ggtgcgttcg gcgatttctt tttgggtctg ttcaaagagt
     8461 cgggcattgt caatggcaag ggaggccagg gctgcgaagc gctccattaa attgacgtga
     8521 tcgtgtgtaa aggtgcgttc ctgttcgaca aatgaaatgg ccagcacacc gatgaccttg
     8581 ttccccactt tcagggggac acccatcact gcattgaaac ccgcatccac gaactctggc
     8641 aggtttttct cccattcacc ataattttga atgaccaaag tcttgccgct gccccaaacc
     8701 tcccctgtca ctcccacatt tttttgtgtc aggctgccat cgtaacggga gagtacgcca
     8761 tgccccaatt cctgtcgcag agcggattcg tcgggcagga cgaggtcgat caacccatgt
     8821 tgggtatcca gcagggcaca agcccgtgaa aggatggatt cgagcaaggg cctgagttca
     8881 gaacggttta acaaacccag agcagtctca tttaaagcgg taaggtagtc ggcttgtacc
     8941 tgtaatttcc gctcggcgcg caggcgttct tttaattcca gccgcgcatc atacagggta
     9001 cggttcaaac tgttgatcaa aagatagatg agtgtgcctg caaaaaggaa aatgaccgtg
     9061 aaatcgcggg caaggttcaa cggcgtatcc atgtgttgaa cacggagtcc ccactcttct
     9121 tcaactgcaa agaaccaaag tgctgaaaga ctgataaccc caaagaacaa gccttcacgc
     9181 catcccagga tcaaggctga aagcagaatg ataagaatat aggaaatgac agcggcatca
     9241 cgaatgccgt ctgcggccca tgcctgaaag gtgatgatga cccaaaatga ccccgcaaga
     9301 aagatgcttg caccacgaat gtagccgcgg cgaatgatga cctggacgat tcccagcgca
     9361 accacgatgg ctgtcaacac cagcaatggt atggggtttt cttcctgccc aatgatcgcc
     9421 cgtatgatga tgatgattaa aagaatactg attgcactcc acacaaagct gttgagaatt
     9481 tgcgcggtgc gggttttttc ctcattttca aaaatggggg atgagagcag tcggcggaac
     9541 cgatttggca tcctcgatat tatatatcat gtacctacaa gtaagctctc aaaaaagtaa
     9601 gcaaattttt tagcttccat gttgaaccga tggacgatat tcaagtaaaa cgtcgaaaga
     9661 taattaatat atgatgaccg cagacggcag aacgtggtta tcgattcgga attttaggaa
     9721 agtgctggct taataacagt gaagaacttt ataacttgcg aatccatctt ctctcggtta
     9781 tccttgcaaa cgggagtggt taggtcccgg tgggcttcct ggtcttcaac acctgtggcg
     9841 ggtcgcgttg cgggccgcgg tgggttcgac tcccatccac tcccgcctat gaattggacg
     9901 atggatgaca ggtggtcttg ttgtccatcg tccgctgtcc attgactgtt gattgaaaag
     9961 gtatgcaatg tcaattcctg aaactgaaat ccttaccaac cttgtgtcgc gggtctttcg
    10021 catcgaagat gtgaccagtg aagatccccg caagggattt ttcctgcgtt accgtggtgt
    10081 attggcgaat gaagattctg cggcgctcta cgataccctg gccgattcgc tttcaccgta
    10141 tggcatcatt ccgctgtttc gaatggagga gggacgtcaa gtcgtttatc ttgcgccgaa
    10201 gccgccagag ccaaagcagg atcgaatttc caccaacatc cttctcttcg ttttgaccgt
    10261 gttcagtgtg atgctggcag gggcacaacc cgaaggcaca atgccagatg atttcatggg
    10321 tcaattgctg atgctggcca aaagtatttt cacgggttgg ccttttgcat taagcctgct
    10381 tggaattttg ttgacgcatg aactggggca ttatttcatg agccgttatc acaaaacccc
    10441 cgccaccctg ccgtatttca ttccgcttcc gctcagcctt ttgggcacga tgggcgcggc
    10501 cattatcatg cgcggaaccc ctaaaaataa acgcgtcctt tttgatattg gtgtggctgg
    10561 tccgatcgcc gggttgatcg tggcattgcc ggtgcttttt tatggtttgt cactttccac
    10621 actgggcacc attgacccca acccgaacgg gttcatcgaa ggcaattcac tcatgtattt
    10681 actggcgaaa tacatcacct tcggacaatt gctgccatcc ccgctggaac cgcagggaat
    10741 tttatactgg ctgcaatatt tcttcacggg tcgccctgtt ccctttggcg ggttggatgt
    10801 cttcattcac ccggttgcat ttgcaggctg ggcaggtttg ttggtgactg gcctgaacct
    10861 catccccgct ggtacgttgg atggcgggca tgtcatctat ggcctttttg gcgagaaggc
    10921 acgcaaggga tttcctttta tcattggcat tctggcgata ctcggcattt tctggagcgg
    10981 atggtggctc tgggctttgt tgcttttttg gctcggcaga gttaatgcag aaccattgga
    11041 tcaaatcacc acgctcgacc cgccccgacg cctgctcggc tatattatga tcctcgtctt
    11101 ccttttggta ttcatgcctg ttccattcat gacactggct caataaatca gcgatgaaaa
    11161 agataatcct ctcatttatt atttttacgt tcgtcctcgc cgcatgcgga acgcaggagg
    11221 atgtcacttc cacggcggag cctgctgtta cgcaggcggc cacgaaaacg ccgcagcctg
    11281 aaccaaccgt cgaagcagcg agcaggcttg gggtcggtga agaagcgttg aatggattgg
    11341 agatcacagt ctggcatccg tggtacggag tcgaagccag tctgttcgag tcatttgttg
    11401 aagatttcaa cgaaagtaat tcatggggca tcaaagtggt tgcagagagt caggtcagtt
    11461 ttacgaacct gtacgaaaac ctgatggctt ccataccggg taaggatcgt cccgatctgg
    11521 cgatcgccct gcccgaacat gccctggagt gggataacaa cggcatgtcg gtggacttga
    11581 tgccgtatgt gaatgaccca gcctatggtt tggattcaag caatatccct gttgtatttt
    11641 ggaatcaaga cctcgatggc gaaaaacgag ttgcgctgcc agctcagcgc acggcgcgct
    11701 ttttgctttg gaatgagacc tgggccgatg agatcgggtt tgcctctcca cccgatgcgc
    11761 ccgatgattt tcgccaacaa gcctgccgtg cccagcaatt catgttaaag gatgaatcgc
    11821 cgcagaacga tttcaaaggc ggttggtatg ccgatacaga ttacatgacc gcttatgcct
    11881 ggctgctggc gtttgaaggc ggtgtgttgg aaggcagtga ttatcgcttt ctcaccccga
    11941 acaacatcga agccttcaaa tttttacgtg aactttccga atcgacctgt tcctggcagt
    12001 cggcagatac tgatgcggtg accgcatttg cgaatcgcga ggccttgttt atcactgcca
    12061 gccttgaaga cctgcccgat gccgcccgcg catttgcaag cgcaggcaac accgatacgt
    12121 ggaaggtcct ctccttcccg ggtgaaaacg aagatgcgct ggtggtctac ggttcttcct
    12181 acgttatgct gaaatcaacg aacgaagaac aactggcggc atggttgttt gcccgttggt
    12241 tgacagatga aaaacaggat gcacgctggg tggaggcgac tcatctcttt ccgcttcgca
    12301 ctacgaccct cgacctgctt gccgattaca agaagactca cccccaatgg gaacaggcgg
    12361 tcaaattgct gccacagggt gagatccaac cgcaattgga ttcctggcga aaagtgaaga
    12421 tcatgcttgg cgatggcttc acccacatgt atcgggtcaa cgtccccagc ggacaggtgg
    12481 ccgccatcct tgcgcaaatg gaagcccttt caagggagtt gagcagatag atcattcagc
    12541 gcagagcatg ttgtgccaga tgagtgagaa tctacttaac tgcgaaactc gccaagagtg
    12601 cccttaaatc tttgtgagct ttgcgttctt ggcggtgtaa ttattttttt cgcttatctt
    12661 gatgtgtaaa tttgggccgc cgtgatgata tttttattga agctggtaag gcgctttttt
    12721 tataaaggag acttgtatgc ctgaatcaga atatcgcgcc cgacaagtcc gcgcgaaaac
    12781 aaacgaagtg ttcgagtatg accgtgatga tgaaaagccc ggcaaacccc tgcatatttt
    12841 gatccccggt gggttgattg ctctggcaac cctggcattg atcgttttta tcgcgtatca
    12901 aacgtggttc gagggaaatc ttgcggagaa catcggcctg attttggtcg tcatcctttc
    12961 tccgttctac gtcggcggag tgttcctttt tagttatgga tacgagcttt acaacattcc
    13021 gcgcgccctg cggttgacgg cgatcatcgt gtttgtgacc ctggcggcgg ttgtgatcgt
    13081 agccgtgttg ttctttctgc tggcaaatat gaatggcagt tcgggttcca aatcttcttc
    13141 ttcaaattct tccaaaggtg gtaaatcacg gtcatcttca tcctcgtctt ccagtaagcc
    13201 atccaattcg ccaagaccct ccacgggcaa aatgggcgga attgtcggcg cggctggcgc
    13261 ggctttgaac agatcgtcca ccaactcttc ctcctccgat tcatcttcct ccagcggata
    13321 ttcttccggc ggacatgtgt atattggcgg cggggaaacc cgtgaagtga caaaggaagt
    13381 ggtgaaggaa gtgcccaaag agccaatgcc cgtcacctgt ccattttgcg cacggtctta
    13441 cgtgcccgtg gaaaataact atatctgccc aagctgtgga gcggcgacac caagggaatt
    13501 gatcaaagag tcgcaggaac cgaagtaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnt gtggagcggc gacaccaagg gaattgatca aagagtcgca ggaaccgaag
    13681 taaagaatgg aaaacgaggt gtgttgatgc tgggtgtgat aatcacataa catcttcacg
    13741 gtataatcag aaaaggcagc ctttatgaag agttcagaat ttgagtggga tgatgaaaag
    13801 gccgagcgta atttcaagaa acatggtgtc ggttttgaag aaggagcgac tatcttcaat
    13861 gaccccatga tagcaaccat tgatgaccct gaccattcgg aaaatgaagc gcgttttatt
    13921 tcaatcggta tgtcagttgt caggcgattg ttgactgtca ttcacacatt tagaaaagaa
    13981 agaatccgtc tgattagtgc aagaaaagcg acgaaaaatg aaaaaaaata ctacaaaagc
    14041 cattaagaaa acaaagaata atgacggacg ttctgaatat gatttcagta aggctcagcg
    14101 tggcaggttc taccgcccac tggataaggg gtacacagtt catgtgcgta aaagtgacgg
    14161 aacggaagtc gtcaatcatt acacattaaa agaaggtacg gttttacttg cgccagatgt
    14221 actcgaatat tttccagatt ctgagtctgt aaatgaggct ttgcgttccc tgattcactt
    14281 aatggagaat gtccccgtga aaaagtatag tgtttcgaaa atggctacac atcaagtggc
    14341 ggagagaaaa taaagatata gacccttgtt gaaaacttac tcggcgtgga agttgcttcc
    14401 cgccgaggat ttatttacag gaggcaacca tgcgccagat gcttatcaat ggaaagttca
    14461 caaatggaaa tgcaacggaa gagatcgaag ttcaaaaccc tgccacagaa gaggtgatcg
    14521 agtcggtccc gcgcggcacc ccgcaggatg tggaggatgc cgtacaggcg gcgaaatctg
    14581 catttgattc gtggcgcaag ctggggtcaa atgaacgtgc cagattgttg cacgaagtgg
    14641 cagacagggt tcatgaacat cgtgagaaga tcgttcacat gctcaccctg gaagaaggca
    14701 aacccgtttc tgaaaacgaa gaagaagtgg aatgggtcat caacaccttc cactactacg
    14761 ctgagttggg ccgtcatcat cgtggcagtg tactggcttc gggtgaggct tctcaattca
    14821 atttcatcat caaggagccg tatggtgtgg tcggctgtat tgtgccgtgg aattatccgc
    14881 tgctcctgct ggcatggaag gtcgcacccg cacttgcggc tggcaatacc gtcgtcatta
    14941 agccgtcaga aatgaccccg ttgacggcac tatatctggc caagcattgt tttaatgggc
    15001 ttcccgcggg tgtggtcaat gtggtcacag gctatgggct tgaaacaggc gaaccgcttg
    15061 tcaaacaccc cgacgtacct gtcatcgctt ttacgggcag tctcgcaaca ggtcaacgca
    15121 ttgcttcgat cgccgcacca atgatgaaaa agctgcacct cgaattaggc ggcaaggatg
    15181 ccaccgtcat tgcagaggac gccgaccccg agatcgccgc caaagctgtg gcatacgcgg
    15241 ctctcatcaa cgccgggcag gtttgcacat caacagaaag agtctacctg ccgaagactc
    15301 atgcggcgaa attcaccgaa gccatcgtgg agcacgtcaa atcgctcaaa ctgggccccg
    15361 gcctcgaccc caaaacagac atgggaccca tgatcggcga aaccttccgc gccaaatttg
    15421 aaaagcaact tgtggatgcg cgcgccagcg gggcaaagat actcaccggc ggtgcgcggc
    15481 cacaatattt ggaaaagggt tttttccacg aaccgaccgt gttgacgggt gtgaatcaca
    15541 gcatggtcat catgcgcgag gaaaccttcg gccccgccgt gccgatcatg gaatacgcct
    15601 cttttgacga agccattcaa ttgaccaatg actgtcaata cggtctggga gcggttttga
    15661 tctccaacga cccgaaaaag atcaaacgct ttttcgacga tgtcaaagca ggcacgatct
    15721 ggatcaacga ccccctgacc gataactacg ccggtccatt cggcggcatg aagtactccg
    15781 gtggcgcacg cgaacttggc gaggaaggcc tcgaagaatt ccgcgagacc aagcatgtac
    15841 attgggattt caatatggaa gaaaaaggat attggtttcc atacggacgc tagcattttc
    15901 atcaaatacc agccagtagt cagtccagtt tataatctga gccatgagaa aacaatcatg
    15961 gctctttcct tttgtattgc tcctggcagt gattttgtat ttccagattt cgcgtgtgca
    16021 gttacccggt gatggatttt ccaccttcgg ctcggatacc gtccgtgcgg aggtgataca
    16081 gattattgaa gaaggcgaag tggaccttgg tggtcatacc cagaaatatc aaatcgcgcg
    16141 tgtgaatatt ttggaggggg aatatgaagg cattccaatg gaaattgact atggcaagcg
    16201 tcaacttcgt tcagatgatt atgaccttgt cgtaggcggc agggtgatgg tgtccattag
    16261 caagacaccg gacaatgtag ttaatgccta ttttgtcgat ttcgtccgca cttctccgtt
    16321 gttgtggttg acggtcattt ttgcggcggc aatcattctc atcagccgct ggaagggggt
    16381 gcgcgccttg ttgagtatgg catttagttt gtatgtcatc attgaccaga tcatcccaca
    16441 tattctcaat ggcgatgacc cgctttgggt gagtattatc ggttcgatcg tgctgctgag
    16501 cgttacactg tacctcacct atggctggaa tttgaagaca cacgcctcag tgctcagtat
    16561 ggtgctggta ttgctgctca cgggggcgct ttccgcggtg ttcgtcattt tcaccaagtt
    16621 gaatggcaca ggggacgaaa acgtcatgtt cctgatgcaa atgatggaaa aaccgctcaa
    16681 cctgcgcggg ttattgctcg gcggtatgat cattggcgca cttggcgtgc tggatgatct
    16741 tgtgacgacg caatcggcgg cggtgttcga gcttcaccat gccaacccga gttttaagtt
    16801 ccgagacttg tacaactcgg ccatgcgcat cggtcaggac catgtggcgg cgacggtcaa
    16861 tacattggtg ctggcgtatg cgggagcgtc tttgcccatg ctgttgatgt tctctttagg
    16921 gcatgggaat tacggctaca ttatcaattt ttcgtttatt gctgaagaag tggttcggac
    16981 tttggtcggc tcgttggggc tgatcgcggc tgtgccctta accacagcca ttgccattct
    17041 ttttgctcaa agggccgagt tgcttggacg gtgggagcag gttctcggtc cagaaggaag
    17101 cggggatggg catggacacg cacactaact gcgcataggc gcttgggctg acgaggggga
    17161 atccagtaat ttgcatcccg cttggcgcaa ctttctctca ccatccgcgt aaaagaagta
    17221 gaaagtctac gaggagcacg cgtgaacgaa gaacagacct gggtgatcca ggcacaacaa
    17281 ggcagtgacg aagcatttac tcatctcgtc gaaacctatc agacccctgt tttcaacctg
    17341 tgttatcgca tgttaggcga accagatctg gctgaagacg ccgcgcagga gacgtttttg
    17401 cgcgccttcc agcacctgca tcgctatgac cagaaacgtt cgttcgctac ctggctgctt
    17461 tccattgccg cacactactg tattgacagg ttgcgccgcc gtaaattcac tattttttcg
    17521 atggatacag aggacgagga cggtaacacc tttgagttac ctgacctcga ttcacccaac
    17581 ccggaagctg aatccatcaa agggcagact cgtgaccgga tccaggccat gctcaaagac
    17641 ctcgacccta ctgatcgcgc tgccatcgtc atgcgttact ggtacgacta ttctgaagtt
    17701 gagattgcag actctttacg attgacggtg agcgcggtga agagccgttt acaccgcgca
    17761 cgcaaggaac ttgctggtct ttggcaggaa gaagaaaccc gtgctgagac cgaaaggaga
    17821 ccctatgaat caccagcctt ttgaagaatg gctactgaac gataagaact tgaattccgc
    17881 cgaaaagcgt gaactcgatc tgcatttgcg gacttgtgcc cattgcaccg cgctttctgc
    17941 cactggtctg gcattgcgct ctgccaatgt ggttgcacct gcttcggggt ttgccctgcg
    18001 cttccaacag cgtttggctg cacaacgaat tgccgaacgc cgccgcaagt tgtggggggc
    18061 gatggtgctg gttttgagcg gatttgccct gctgacatgg tttgtggcgc catttgtgta
    18121 tgcctttcta aattccccgg cagaatggat tacggccgca gtgagctatt ttctattctt
    18181 tgcaagctca gttcaagcgt tgaccgaagc cgccgccgtg ttattccgca ttctgcctga
    18241 ctttgtcccg ccttatatgt ggatggtgtt cgtttctgcg ctggccggcc tgggcctttt
    18301 atggacaatt tcaatctggc aatttagccg caccccgcaa ggagtttcag catgagaagt
    18361 aaattaacga ccctttttct attactagct ttattgtttt tacccactgg agctgtccac
    18421 gcgcaaagcc cggacggtgg cgatgttttt ctattggggc agaactacac ccttaagagt
    18481 gatgaaacgc tggatggcag cctggttgtg atcggcggta atgtggatat cgaaaaagat
    18541 gccacagtga acggcgatag cgtgctgatc ggcggcaatt tacaagtcaa tggagatgcc
    18601 aacggcagtg tggttctgat cggtgggaac cttgtgatca gcggaaaaat caaaggtgac
    18661 attgttttga tcggtggtca ggctttgtta accgaaaccg ctgttgtaga tggcgatatt
    18721 gtgacaatgg gcggtcaggt gcaaaaagat cccggcgccg aagtaaccgg ccagatcgtg
    18781 aataacgctc cgccaattga tgttcctgat attcctgacg tgccaaatgt gcccgacgca
    18841 cccgatgccc ccgatgcgcc tgatattcct gatgtccccg gggtgcctca gatcaacgtt
    18901 gatttcaacc cgttatggga agtgacaggt gttttcaccc gtgccctggc cattgccgcc
    18961 gttggcatgc tgctgaccct cttcctccag ccgcaattag atcgtgtggc agatgccatt
    19021 acacgtcagc cgatgatggc gggtagtttt ggcctgctga ccgtggtcgt tgccccggtg
    19081 gcgatcctga tcatgatcgt gaccatcctg ctcatccctg tggcagccat cgtggccttt
    19141 attctgcttc caatggcctg gttgtttggt atggtggcca tcgggcagga gatcggtgac
    19201 cgcttcacca aggccatcaa tcaaacctgg gctcctgtac tttcgacagg ttttggaacc
    19261 ttcctgttcc tgttggtcgg cggctttatt gggctgatcc cctgtatcgg gtggctgatc
    19321 tcccttatgt tctcactcgt agccctcggc ggtgtgatga tgacgtggtt tggcacccgt
    19381 gctgcccccg gctcggctcc tgcggtggta acagaaacga tcccgcctgc ttcgtaaata
    19441 gccgttttca aggttgtctg gcgagagttg ctaagtctgt gggctaacca caaagacgcc
    19501 aggccactaa gccaccaaga atcctttgtg tcttcgtggc aatcaaaacc ctttagcttc
    19561 ctgtttggca agccaaagcc gaaaaccaaa cctgcatgat ataatgcgcc cgccccgggg
    19621 aagttccccc ggggcggttt tcatgatgga ggaaaacatg tttacaaagc aagaaattcc
    19681 caatacccca attcacaaca ccccgccgcc tctgatcgag gtttcaggca cacaccgcga
    19741 gatgggacgc cagatcggtg aggccgcccg cgcacaggtg cagcatagcg tggccaatgc
    19801 ccgcgttttg atcgacgccg cctacgacag ccttgaactc acctggcagg gcgcgcagat
    19861 ccaatcacga aaatacctgc ctttcgccga ggaacgctac ccgcaatatg tggatgaaat
    19921 gcgcggcatt gccgagggcg caaatgttgc cttcgatgac attgtcacgc tcaatgcaat
    19981 ggaagccgtc accgtggatg cacttcacct cacccgctgc accagcatgg cagtcaacga
    20041 agagcgcaca gc
//
LOCUS       DAOQLC010000079        19984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_942_length_19984_cov_27.930654, whole genome shotgun sequence.
ACCESSION   DAOQLC010000079 DAOQLC010000000
VERSION     DAOQLC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19984
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_19984_cov_27.930654"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PL000_13890"
     CDS             <1..507
                     /locus_tag="PL000_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90041.1"
                     /translation="RKVVGLLAQRPTPLPMSIYDNIAYGMHIHKMKNDRREYKAAVRH
                     YLEIAGLWEEVQKRLHDPATSLSIGQQQRLCLARGLAVEPEIILGDEPTSALDPISSQ
                     NIESRLIELKQQYTTVIVTHTLRQAKRLADYVIYMYLGEVIEAGPAKEVFSNPKHERT
                     RQYLEGQI"
     gene            812..2788
                     /locus_tag="PL000_13895"
     CDS             812..2788
                     /locus_tag="PL000_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC90042.1"
                     /translation="MSISENNQKEIMHRESDYKPPRSLSHWMIGRPLSTADAPHQTIG
                     KAVGLAVFASDALSSTAYATQEILGVLAAAGTIAFGYVFPISIAIVVLLAIVTISYQQ
                     TIHAYPGGGGAYIVARDNLGELPAQIAGAALLTDYILTVAVSISSGVAQIVSAYPNLE
                     DYRVQIAVGAVLLIMLVNLRGVKESGVAFAVPTYFFILMMFTTVGVGLFRSFSGTLGS
                     VINPPELDIMHSVSGISAFLILHAFANGTTALTGVEAISNGITAFNEPRSRNAGITLI
                     WMSLILGALFLGISHLTGQIGAVYSEEETIISQLARTVFNGRGLLYLSAIWGTTVILI
                     MAANTAFADFPRLGALAASDGFLPRQLTFRGSRLVYSNGIVTLSVIASLLIIAFKANV
                     TLLIPLYAIGVFMSFSLSQFGMALRWRKIGRLKEGEEIRERGSVLHYDSKWKSKMLIN
                     GFGALCTLIVMFVFAVTKFSEGAWFVLILTPILVIVFFAIHRHYSSLAHSLSLEKFDG
                     LPARQTRHRVILPISGVHQGAIEALRYARLLSDDVTAVHISLDPAETEKVQKKWITWG
                     EGTRLVILDSPYRLFVEPLLEYLKDIIKNRQPNETITVVVPQFMPSKRWHNLLHMQTA
                     NVLRSELLNQHGVVVTDVPYHVHEKHTEHQEVIK"
     gene            2785..3447
                     /locus_tag="PL000_13900"
     CDS             2785..3447
                     /locus_tag="PL000_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNC90043.1"
                     /translation="MNLIVVGCGRVGAELAYRLFKGGHQVTIVDSNRQAFNRLHPDFR
                     GRTLEGEGLAENVLDRAGIKEADGLAAVTNSDTLNAVVAHTARIYYHVPVVVARNYDP
                     NLRPVIESFGLQTVSSTFWGAQRVEELLINPAQRVVYSAGNGEVEVYEVSIPESWNGR
                     TLKELLDPLKNCFAVAVTRAGRSSLPDLGMVLQTGDMLNFSSTYDGIGALTSRLSGKA
                     EA"
     gene            3449..4147
                     /locus_tag="PL000_13905"
     CDS             3449..4147
                     /locus_tag="PL000_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC90044.1"
                     /translation="MFVLITGGGRTGARLANLLLNEKYKVRLVENRRNLLSLLHQELP
                     TEVIYEGNPSDPSVLEAAGIREAHAVASVTGDDSMNLAISFIAKQMFGVPRTIARVNN
                     PNNAWLFDEKFHVDVSLNQADVLAHLIQEEMSLGDMMTLFKIRRGRYAVVEEKVPAGA
                     KAIGVQLREMGLAEHCVIAAIIRDGVMTLPRGDSTLKEFDEIIAVASPEGAKRLAELL
                     ADPAHPDRNDGRKA"
     gene            4296..6098
                     /locus_tag="PL000_13910"
     CDS             4296..6098
                     /locus_tag="PL000_13910"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNC90045.1"
                     /translation="MAKEFHYTWVWELESSPEALWPLASDTNRFNRDTGLPPMELLGI
                     SNGAKLVRFKVPLLDIRWEEDPFEWTYPYRFGILRRYKSGPLAEMRVDCRLEPRQPSG
                     TRIVYDVVVKAANILGDIGIPLAIGKVSAKRFEDTFKRYDRISANIGSIVMNIKGRNL
                     SSAGRTKFKVLSDRLKSQGVDTVILDHLFEYMNNADDLSIQRMRPYALADGWKVNRRT
                     VLETFLRATRVGLLDMYWDILCPECRGVTDDKAHLGELQSTAHCNTCQIDFTANFDHN
                     VEVIFRPNPSVRPVDATVEFCVGSPQRQPHIAFSLTVPPREELPFSTILNEGRYSLHA
                     SDVPGTQIILASNDGVEQIDFRSRITGWPTGITKLGLNPGVRLINETDSTQTFELVRT
                     AWSDQAATAADVTTLQVFRDLFSSEVLRPGEEISVGATTLMFTDLRNSTRMYREIGDA
                     PAFGRVREHFEILEQAVTAEGGSIVKTMGDAIMAAFRNPASALRAVWTVQKQIAARGE
                     PPLSLKVGIHHGPCIVVNLNDRLDYFGSTVNIAARLPNFSTGGEAIISESIRNDQEVI
                     EFLEKNAPPNSLTRFQGDIRGYDQSMDLWRIQMQ"
     gene            complement(6111..7319)
                     /locus_tag="PL000_13915"
     CDS             complement(6111..7319)
                     /locus_tag="PL000_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC90046.1"
                     /translation="MHILIIHQAFASLNEPGGTRHHEFARLLTAHGHQVTVIASPVSY
                     ITGSAFRASVSSEVTEDKVTILRASVYDAHHRSFLHRIVAFFSFMLSSFWVGLGVKNV
                     DLVWGTSPPIFQGVTAWVLARIKGAKFLFEVRDLWPQFAIAVGVLKNPVLILLSEWLE
                     RFLYRRADRVMVNSPGFLEPVTSRGAKRVELIPNGADPSMFDPNNDGMEFRRSNQLTD
                     KFVVLYAGAHGMSNDLEVVLDAAALLVDRNEIQLVLLGDGKEKPALMARAQKMDLKNV
                     TFLPSVPKTEMASALAGADACLAILKPLEEYKTTYPNKVFDYMAAGRPVVLAIDGVIR
                     EVVEAAGCGIFAAPGSADEVANAIRQLAADKKESRIMGLQGRDYLQKNFSRAAIGEKL
                     LGLLEDLRSK"
     gene            complement(7361..7804)
                     /locus_tag="PL000_13920"
     CDS             complement(7361..7804)
                     /locus_tag="PL000_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC90047.1"
                     /translation="MRIRSGIILIEENKVALIERHRAELHYFTFPGGGVDEGESPEQA
                     AIREAEEELGIQVKLKQKAAEVQFGGRLQVYFLAEKQSGEFGTGTGEEYGEYDPLHGT
                     YQPLWMPLEDMKSRNVVPRELADLVLRFVKEGWPLEPVIIVEEKR"
     gene            complement(7854..8771)
                     /locus_tag="PL000_13925"
     CDS             complement(7854..8771)
                     /locus_tag="PL000_13925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNC90048.1"
                     /translation="MSHYRSLEEVSIQNAWLTIGVFDGVHRGHQQIIHKLVQQAHAQG
                     QPAVVLTFAPHPAAVLGGRDIKCLTTPEERADLLMAMGVDHVITQRFTRDLSTASAKE
                     YMTQLKQSLGLEHLLIGYDFALGRGREGNASRLSEIGNELGYTVEIINALSDESGVIS
                     STEIRKLVSTGNVTEAAKLLGRPYALSGEVIRGAGRGRTINFPTANVDYVQQKVAPSN
                     GIYACWAYLGTEKFMAATNIGFNPTFTPDRQTPSLEAYLLDFDRDIYGLNLKLEFVSR
                     IRDELKFDSVEALIKKIHEDVDQTRELLS"
     gene            complement(8868..9791)
                     /gene="truB"
                     /locus_tag="PL000_13930"
     CDS             complement(8868..9791)
                     /gene="truB"
                     /locus_tag="PL000_13930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC90049.1"
                     /translation="MNSQDVKNAISGALVVDKPVGMTSHDVVQAIRNGTGLRRAGHTG
                     TLDPRASGVLVILVGPAVRLSEYVSASDKRYQAIIRLGGSTDTFDAEGKVTPTKDPVN
                     VTETQFEEALKTFIGEIEQTPPPYSAVKVQGRKAYEMARQGEEVDLAPRKITVHHLEV
                     LEWTPPEVVIDVHCSSGTYVRSLANDLGVMLGCGAYLVGLRRTKSGRFSLRDSVPLRK
                     LQEAFTAGNWYQYLIPAAEALGDWPAVELNPDEVEGVRHGHRVKVVGEPKETKVRGVS
                     TQGELVALMELITTEDGSHEWQPKKVFFTSD"
     gene            complement(9885..10841)
                     /locus_tag="PL000_13935"
     CDS             complement(9885..10841)
                     /locus_tag="PL000_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNC90050.1"
                     /translation="MDNQISGEIKNRLSAAKEIVIVSHVRPDGDAIGSLLGLGLALRE
                     AGKSVQMVLVDGAPASFKYLEGSQLIVKEPQGNHDTFITVDCADFKRTGKIFENFGQP
                     DINIDHHKTNEKFGKLNLIEADEVATAAILTKHLPVWGLKITKAVAAALLTGIITDTL
                     GFRTSNTTPDALRQAATLMESGVDMPEIYMRALVNKSLPAARYWGAGLSSLESRNGVV
                     WGTLTLEDRKSAGYGGNDDADLINMISAIDSHKVGMVFVEQSDHHVKISWRALESGLD
                     VSQIAKHFKGGGHAAAAGADIEGNLQDVQKEVLAKTFEMLNL"
     gene            complement(10909..11259)
                     /gene="rbfA"
                     /locus_tag="PL000_13940"
     CDS             complement(10909..11259)
                     /gene="rbfA"
                     /locus_tag="PL000_13940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNC90051.1"
                     /translation="MPSGIRLQRIADRVRQELSELLIREVNDPRLHQIFVTDVTIDRE
                     LAYADIYVSAIEGAGRSAEILEGLESASGFLRRTLSARVEVRAFPRLRFHWDPTPEHA
                     DHIEKVLAGLREKK"
     gene            complement(11320..13098)
                     /gene="infB"
                     /locus_tag="PL000_13945"
     CDS             complement(11320..13098)
                     /gene="infB"
                     /locus_tag="PL000_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC90052.1"
                     /translation="MSSNGNKLELPASIVIRDLALKIEKSPIDLIKKLMSNGVMATIN
                     QTVDFDTAAIVVGEYGFEAVLETVEEEESKAEVGEVPLWRQMIAGENNAFLKPRPPVV
                     TILGHVDHGKTSLLDAIRSTEVAAGEAGGITQHIGAYQVEKKGRLITFLDTPGHAAFT
                     QMRARGAQGADIVVLVVAADDGVMPQTREAIAHAKAARVPLMVALNKVDKANANPDRV
                     KQQLAELELVPDEWGGNTMVIHVSAKQKQGIDDLLEGILLVADSSEIKANPNGKVIGT
                     VIEAELDKSKGVLATLLVQNGTLEASDIVVAGTAYGKLRAITDYKGKPIKKAGPSTPV
                     AVMGLSGMPSAGDIFEVVDSEKEARNIVNERVEAAKTLSQTRKKVSLEDLFANVQAGE
                     AKGLNLIIKADVQGSLDPIIGELNKLGEGEIGLHILHAETGNISNNDVMLAAASRAII
                     IGFNVQADVDGRRLAEKEGVDIRLYEIIYRMTEDIEKALKGMLEPKLVEKVIGRAQVL
                     AVFSASKFGKVAGCKVTDGELKRGARVRLFRGTDMIYEGDLSSLRHEKEDVKEIRQGY
                     ECGVGFKSFNDILPGDQLVCYIVENS"
     gene            complement(13207..13461)
                     /locus_tag="PL000_13950"
     CDS             complement(13207..13461)
                     /locus_tag="PL000_13950"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HNC90053.1"
                     /translation="MGCREVLPKRQMVRIVRTDNGVWVDPTGKAAGRGAYLHDQRECW
                     VRGLRGALGHALKAELTADDRARLEEFMNSLPLSEAEAGK"
     gene            complement(13530..15422)
                     /gene="nusA"
                     /locus_tag="PL000_13955"
     CDS             complement(13530..15422)
                     /gene="nusA"
                     /locus_tag="PL000_13955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC90054.1"
                     /translation="MAKNEFALAFNEVLEEKQLAKDVVIAAIESAMVSAYRRAVSAST
                     AQHVEAKLDPDTGEVTVYAEKEVVEDSVVDARTEVLLGEAQKVKPDTQVGDMVLVETT
                     PADFGRVAAQTARQVIQQRIREAERSNQMQYFERQVGEIVSGLVQASNAQSATIGLDM
                     KAEGILPNNQKIPGERLKLHDRIRAVVMEVKDGTRGPQIILSRAHRNFLRRLLENEVP
                     EIYHGIVEIRAIAREPGARAKVAVSATQPGIDPVGACVGIKGVRIQAIVKELHDEKID
                     IIQWDQDPITYISKAISPARVNGVYLVDTEGARTATVVVGEDQLSLAIGRDGQNARLA
                     AKLTGWRIDIKSLTEAASDTLDKLQANAELGAMLPAAMEAIPQVQEVLAKKAEGRAIT
                     PEEYTVLGQFIDRVERRTIQIKEEAARVEEERTVAARADIPAAAFQMPLDQAGMKEHI
                     FNILTEANFETVGQLMFVLKTEPNKVLGLPGIGSKAMQNIEESLAALTFPEPEKVEEA
                     PVAVVAAEPVVVETAPVEPAAAAEETPVVKKQAEGKRDAKRPVEVEEDEEHAKDGVSL
                     DELFKMKPEIFQGARPAEEEVSGDKKKSKKGKKKAVELEFDEGLGTVVGRKIHKRGGD
                     VEGDWE"
     gene            15742..17127
                     /locus_tag="PL000_13960"
     CDS             15742..17127
                     /locus_tag="PL000_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNC90055.1"
                     /translation="MSAETKPAVIGFLPDDTPPIGAMLSLGFQQFLTMFPATVLVAIL
                     TKFDVGITLLASGLGTIIALLVSKRKIPMYYGSSFSYIAVIITVMNKFAPDCYNSAAT
                     YCPDGVRIVQVGIILTAVFEILIGLLIMRIGKAALDRVLPPIVTGSMAMVIGIALANA
                     ALGNAGNWAAPELAATTWTVAFITLIATVLFSVYLQGKGLIGMLPILFGAIFGYLVAI
                     PFGLVHFENVASASWLAMPKFTLPAFENPEAWGMALSIALIFIATVPESTAHLYQMSL
                     YIDELAAEMKRKPYNIKELIGLNLVADGSDDIVVGLLGGCAGTNYGENNSLMAITRNY
                     STAVLMTAGAMAMVFGFFGKLVALVNTMPAAVVGGLSIYLFGVIGMQGVALIQSEKVN
                     LFDPKQLAVGAIILICGIGGNLALPNGVFPFNVPFLFPNGIPAIVFAAIVGIFINTIF
                     LIFKTPEVRGG"
     gene            17215..18291
                     /locus_tag="PL000_13965"
     CDS             17215..18291
                     /locus_tag="PL000_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC90056.1"
                     /translation="MIKLPGLIDPHVHLREPGATHKEDFDSGTSAALAGGVTIVLAMP
                     NTKPPIFDAATLDLALDAARQKARCDYGQYVGAGPDNSDWISHKAESDRAAGLKMYLD
                     STFGELRLDDMSLWTPHFANWPKEMPIVAHSESRSMAAAILFAAIHDRPVHIAHISLK
                     EEVLLIKAAKEHGLKVTCEVCPHHLFLSKDDIPAISHGHPGRGEVRPRLATKADVEAL
                     WANMDVIDCFATDHAPHTIAEKDSDNPPPGFPGLETFLPLILTAVQDGRLTMDDVISK
                     MYTNTKHIFNLPEQPETWVEVDENAQYEIKAAEQFTRCHWTPFEGWKVKGRVRKVVLR
                     GKTAFEDGKILAEPGYGKNIRSFN"
     gene            18508..19917
                     /locus_tag="PL000_13970"
     CDS             18508..19917
                     /locus_tag="PL000_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90057.1"
                     /translation="MNKKAFIVLLLAAIVLAQFSLASAAAVSGDASITSVPTFLPLKQ
                     NLDVEWAVSGLPAGGPRYLLMWARREPSTTYDMAECKVVENLTGLTEASGAFEYSFAG
                     TNIAFLMIDGDVIETTLTLDTDDCAGTPDLPADSSVMGSTTIDNKVPSMRAGNPPSLS
                     AANIMLQPVACNTFEMWAVASDIAGLDPDAPYSGLVSWGQTTNGTFTPAAPIGKAEDT
                     LSWVYTFPATATGQWTFYIHPKDAAGNSGLLGNIYRLTLPIEPQELEDCATFSDVSGH
                     ADEVYIRYLATLGLINGFADGSFGPDNTLTRAEAATLFEISNGYDENTLPASAPAGCE
                     FSDVTASDWFAGWVWQACADGFMNGVGGGLFDPNNLLTRGQIVTIMNNVANSTPAGGY
                     LNTISVVNSLWNHFIFKPILKAAFTDVPVGAYYADPVIQAYGWGVADATSETTFSPDQ
                     PVTRGEFAKMLYRALSRVG"
BASE COUNT         4638 a         5568 c         4789 g         4989 t
ORIGIN      
        1 cgcaaggtgg tcgggctgct tgcacagcgc cccacaccgc tgcccatgtc catttacgac
       61 aacattgcct acggcatgca tatccacaaa atgaagaacg accgcaggga atacaaggcg
      121 gctgtgaggc attaccttga gattgccggt ctgtgggagg aagtacaaaa aaggctgcac
      181 gaccccgcca ccagtctctc catcggacag caacaacgcc tctgcctcgc ccgcggcctg
      241 gccgttgaac ccgagatcat cctcggcgac gaacccactt cggccctgga cccgatctca
      301 tcgcaaaata tcgaaagccg cttgatcgag ttgaaacaac aatatacaac ggtcattgtc
      361 actcacacac tgcggcaagc caaacgcctt gctgactatg tcatttacat gtatctcggc
      421 gaagtcatcg aagcaggccc ggccaaggaa gtcttttcca acccaaaaca tgaacgaacc
      481 cgtcaatacc ttgaagggca aatctaaatc tttatttcca atagaagcag ttggttcgca
      541 tcagcctgtc catgctgtat ggacaggctg attgtgtttt agacaggaga cactctctga
      601 aaacatggaa tcggactata atcacgcctt gttccatttc tcaagcattt atcctctggc
      661 aatatgagca ccccaaaaga aacttccccc aacctttttg acagctaatc aattgtgcga
      721 tcatttcaac caatccctgc gcagtgaaac ttgtttcgaa attcagcgta aagggtgatg
      781 ggttgtgtgt ttttttatag aaagagagaa aatgtccatt tcagaaaaca accaaaaaga
      841 aatcatgcat cgggaaagtg attacaagcc gcccagatca ttaagtcact ggatgatcgg
      901 caggccgctt tccacagccg atgccccgca tcagacgatc ggaaaagccg tcggtctggc
      961 tgtctttgca tctgatgcgc tctcctccac tgcttacgcc acacaggaaa ttctgggagt
     1021 tttggccgcg gcaggcacga tcgcatttgg atatgttttt ccgatttcca ttgccatcgt
     1081 tgttctgctg gcgatcgtga ccatttcata ccagcagacc atccatgcct atccgggcgg
     1141 cggtggtgcg tatattgtgg cccgcgacaa tttgggagaa ttgcctgcac agatcgctgg
     1201 tgccgccctg ttgacagatt acatcctcac agtcgcggtg tccatttcat cgggtgtggc
     1261 gcagatcgta tccgcctacc caaacttgga agattaccgt gttcagatcg ctgtcggggc
     1321 ggtgctgttg atcatgctgg tcaatttgcg gggtgtcaag gaatcgggcg tggctttcgc
     1381 tgtcccaacc tatttcttca tcctcatgat gttcaccacc gtgggtgtgg gtctgttccg
     1441 aagtttctcc ggtaccctgg gaagtgtgat caacccgcct gagttggaca tcatgcacag
     1501 cgtttcgggc atctccgcat ttttgatcct gcatgctttt gcaaatggca ccacagcctt
     1561 gaccggtgtg gaagccatct ccaatggtat tactgcgttc aatgaaccgc gcagccgcaa
     1621 tgcaggcatc accctgatct ggatgtcgct cattttgggt gctttgttcc tcggcatttc
     1681 acatctgacc gggcagatcg gggcagtgta ctctgaagaa gagaccatca tttcacaatt
     1741 ggcgcgtacg gtatttaatg gccgcgggct gctctatctt tccgccatct ggggtacgac
     1801 cgtcatcctc atcatggcag ccaatacagc ttttgccgat tttccacgcc tcggcgcgct
     1861 ggcggccagt gacggcttcc ttccccgtca gttgaccttt cgcggcagcc gccttgtgta
     1921 ttcgaacggt attgtgactt tatctgtcat tgcttctctt ttgatcattg cattcaaagc
     1981 caacgtgaca cttttgatcc cgttgtatgc catcggtgtg ttcatgtctt tctcgttatc
     2041 tcaatttggc atggcacttc gctggcgcaa gatcggacga cttaaagaag gcgaggagat
     2101 ccgcgagcgc ggttcggtgc tgcactatga cagcaagtgg aaatccaaga tgctgatcaa
     2161 tggattcggc gcactgtgta ccctgatcgt gatgtttgtt tttgctgtta cgaaattcag
     2221 tgaaggcgca tggttcgtgt tgatcctgac gccgatcctt gtgatcgtct tcttcgcaat
     2281 tcaccgccat tattccagcc tggcgcacag cctttccctt gaaaagttcg acggactgcc
     2341 agcacgtcaa acccgtcacc gcgtcatcct gcccatcagc ggcgtgcatc agggtgccat
     2401 tgaagccttg cgttatgccc gtctgctttc agatgatgtg accgccgtcc acatttcact
     2461 ggaccctgcc gagacggaaa aggttcaaaa gaaatggata acctggggtg aaggcacacg
     2521 cctcgttatt ctggattctc cctaccgact cttcgttgag ccgctgcttg aatacctgaa
     2581 agacatcatc aagaaccgcc aacccaacga aaccatcacg gtagtagtgc cgcagttcat
     2641 gccttcaaag cgctggcata acctgttgca tatgcaaact gccaacgttt tgcgcagtga
     2701 attattaaac caacacgggg tggtcgtcac cgacgtaccc tatcatgtac atgaaaagca
     2761 tacagaacac caggaggtga tcaaatgaac ttaattgttg ttggatgcgg ccgcgtaggc
     2821 gctgaactgg cctaccgcct tttcaaaggc ggccatcaag ttaccatagt agatagcaat
     2881 cgacaggctt tcaaccgcct gcaccctgat tttcgcggac gaaccctcga aggtgaaggg
     2941 ttggctgaaa acgtgcttga ccgtgcaggg atcaaagagg cagacggcct tgcggctgtt
     3001 accaattcag ataccttgaa cgcggtggtg gcccataccg cacgcattta ctaccatgtc
     3061 cctgtggtgg tggcgcgtaa ttacgacccc aatcttcgcc cggtcattga atcctttggc
     3121 ctgcaaacgg tcagctccac attttggggc gcacagcgcg tggaggagtt attgatcaac
     3181 cctgcccaac gcgtggtgta ttcggcgggc aacggcgaag tggaagtata tgaggtgagc
     3241 attcccgaat cgtggaatgg acgcacgctc aaggagttat tggatccgct taagaactgc
     3301 tttgcagtgg cggttacccg ggccgggcgc tcgtccctgc cagacctggg aatggtcctg
     3361 caaactggag acatgctcaa cttcagcagc acctatgatg gcatcggcgc gctcacttca
     3421 cgcttgagcg gaaaggcgga ggcttaacat gttcgtattg atcaccggag gcggccgtac
     3481 cggagcccgc cttgccaacc tgttactaaa cgaaaaatac aaggtgcgtc tggtggaaaa
     3541 ccgccgcaac ctgctctctc tcttacacca ggaacttcca actgaggtga tttacgaagg
     3601 caacccatct gacccgagcg ttctcgaagc agcagggatt cgtgaagcac acgccgtggc
     3661 ttccgtgacc ggcgatgact cgatgaacct cgccatcagt ttcattgcca agcaaatgtt
     3721 tggcgtgcct cgcaccattg cccgcgtgaa caacccgaac aatgcctggt tgttcgatga
     3781 aaaattccat gtggatgttt ccctcaatca ggctgatgta cttgcacact tgatccagga
     3841 ggaaatgtca ctgggtgaca tgatgaccct tttcaagatc cgtcgcggac gttacgccgt
     3901 ggtggaagaa aaggtccccg ctggagcaaa agccattggc gtccaacttc gggagatggg
     3961 gctcgccgaa cactgcgtga tcgcagccat catccgtgat ggggtcatga ccctgccgcg
     4021 cggcgacagc accctgaaag agttcgatga gatcattgcg gtagccagcc cggaaggcgc
     4081 caaacgactg gccgaactgt tggcagaccc tgcccaccct gaccgaaacg acggtagaaa
     4141 agcctaaccc cctgcctaca tcaatcagat tctaaaaaca aggcctgccc gaaaccaaaa
     4201 attggggagg ccttgttttt ttatcacaac cgcgctgcaa tgcttatgag aggaatcttg
     4261 agtaaaataa tcatgattat gattgtctaa ccaacatggc aaaagaattc cactacacat
     4321 gggtttggga gcttgagtcc tcaccggagg cgctctggcc gcttgcctca gatacgaatc
     4381 gcttcaaccg cgataccggc ctgcccccca tggaattgct cggaatttcg aacggcgcaa
     4441 agcttgtccg ttttaaagtg cccttgttgg atattcgttg ggaggaagat cccttcgagt
     4501 ggacctatcc ctatcggttt ggaattctac gccgctataa aagcggcccg ctcgcagaga
     4561 tgcgtgtgga ttgccgcctc gaaccaaggc agccttcggg cacacgcatt gtttatgacg
     4621 tggttgtcaa agcagcaaac attcttggag acattggcat tccgcttgcg atcggcaagg
     4681 tcagcgccaa acgttttgaa gataccttca aacggtacga ccgtatttca gccaacatcg
     4741 gttcgattgt gatgaatatc aaagggcgta atctctcatc ggctggccgt accaagttca
     4801 aggttttgag cgaccgcttg aaaagtcagg gcgtggatac cgtcatcctc gatcatcttt
     4861 ttgaatacat gaacaatgcc gacgatcttt ccatccagcg catgaggcct tacgcactgg
     4921 ctgatggctg gaaggtaaac cgccgcactg tgttggaaac ctttttgcgc gccacccgcg
     4981 ttgggctgct cgacatgtac tgggatattc tttgccccga atgccgcggc gttaccgacg
     5041 acaaagccca cctcggtgaa cttcaatcca cagcgcattg caacacctgc cagatcgatt
     5101 tcaccgccaa cttcgaccac aacgtagaag tcatctttcg ccccaacccc tcggtgcggc
     5161 ctgtggatgc cactgtggaa ttttgtgtag gcagtccgca gcgccaaccg catattgcct
     5221 tttcgctcac cgtaccgccg cgagaagaac ttcctttttc aaccatcctc aacgaaggcc
     5281 gctactccct gcatgcgtca gatgttcccg gcacgcagat catccttgct tcgaacgatg
     5341 gtgtggaaca aatcgatttt cgctcgagga ttaccggctg gccgaccggc atcaccaaac
     5401 ttggactcaa ccccggcgtc cgtctcatca acgaaaccga ttcaacccag acctttgagc
     5461 tggtgcgcac agcctggtca gatcaggcgg cgacggctgc cgatgtgaca acattacagg
     5521 ttttccgcga ccttttttca agcgaagtgc tgcgccccgg tgaagagatt tccgtcggcg
     5581 ccaccacctt gatgttcacc gacctccgca attcaacacg gatgtatcgt gagatcggcg
     5641 atgccccggc ctttgggcgt gtgcgcgaac attttgagat cctcgaacaa gccgttaccg
     5701 ctgaaggcgg ttccattgtc aaaaccatgg gagatgccat catggcagca ttccgtaacc
     5761 ccgcttcggc cctgcgcgcc gtgtggaccg tgcaaaaaca aattgccgcc aggggtgaac
     5821 cgccgttgtc cctcaaagtt ggcattcatc atggaccctg catcgtcgtc aacctgaacg
     5881 atcggctcga ctactttggc tccactgtaa atatcgctgc gcgcctgcca aatttctcca
     5941 caggcggcga ggcgatcatt tccgaatcga tccgcaacga tcaagaagtg atcgaattcc
     6001 ttgaaaagaa cgcaccgccc aattctctca cccgttttca gggagacatt cgcggctacg
     6061 accaatccat ggatctgtgg aggattcaaa tgcaataaag gggtggaaat ctatttcgat
     6121 ctcaggtctt ccaacaaacc aagcaatttc tcgccaatcg cggcgcgact gaaattcttt
     6181 tgcaaataat cccgtccctg caagcccatg atgcggcttt cttttttatc agcggccagt
     6241 tgacggatcg catttgcaac ttcgtccgcg ctgcccggcg cagcgaatat tccacacccg
     6301 gcggcctcaa ccacttcgcg gatcacacca tcaattgcaa gaacaacagg gcggccagcc
     6361 gccatgtaat caaaaacctt gttcgggtag gtggtcttat attcttcaag cggtttgaga
     6421 atggcaaggc atgcatctgc ccccgcaagc gcagacgcca tttctgtttt gggtacggaa
     6481 ggcaaaaagg tcacattctt tagatccatt ttttgtgcgc gtgccatcaa tgctggcttt
     6541 tccttgccat cacccagcaa aacaagttgg atctcatttc gatccaccag caaggcagcc
     6601 gcatccaaca caacctcaag atcgttcgac atgccatgcg cacccgcata caacacaaca
     6661 aatttatccg tcaattgatt cgaacgacgg aattccatcc catcgttgtt tggatcgaac
     6721 atggaagggt ctgcgccgtt ggggatcaat tccacccgct tcgcgccccg actggtaact
     6781 ggttctaaaa accccgggct gttgaccatc acccggtcag cgcggcgata gagaaaacgc
     6841 tccagccatt cagaaagcag aatcaaaaca gggtttttca atacacccac ggcaattgca
     6901 aattgcggcc acaggtcacg cacttcaaat aaaaatttcg cacccttgat ccgcgccaag
     6961 acccatgcag ttaccccttg aaaaatgggt ggtgacgtac cccaaacaag gtctacgttt
     7021 tttacgccaa gtcccaccca aaacgaagag agcataaaag agaaaaaggc cacaatgcgg
     7081 tgcagaaagg atctatgatg cgcatcgtac acactggcgc gcagtatggt gaccttgtct
     7141 tccgtgactt ctgaagaaac ggaagctcga aacgcagaac cggtgatgta actgaccggt
     7201 gaagcgatca cggtcacctg atgaccatgt gccgtcagca ggcgcgcaaa ctcatggtgg
     7261 cgcgtgccgc caggttcatt gagagatgcg aatgcctgat gaatgataag gatatgcatt
     7321 taacgaaatg gatcaaattt caatgatacg ccgcaaacta tcagcgcttt tcttcgacga
     7381 ttataactgg ctctaatggc cagccttctt ttacaaaacg caaaaccaga tcagccagtt
     7441 cacgcggaac aacgttcctg ctcttcatat cttccaaagg catccataag ggttggtagg
     7501 taccgtggag cgggtcatat tcaccgtatt cttcaccggt gccggtgccg aattccccgc
     7561 tttgcttttc agctaaaaaa tacacctgca gcctcccgcc aaactgcact tccgccgcct
     7621 tttgtttcag cttcacttga atcccaagtt cctcttccgc ctctcgaatg gcggcttgtt
     7681 cggggctttc gccttcatcc acgccgccac cgggaaaggt gaaataatgc aattccgccc
     7741 ggtgacgttc gatcagggcg actttgttct cttcgatcag gatgattccg gaacgtatgc
     7801 gcatgatatg cctcccgttg attctccaaa gctcatggct ttggccctct gattcaagaa
     7861 agaagttctc tggtttgatc cacatcttca tggatctttt tgatgagcgc ttctacagag
     7921 tcaaatttca actcgtcgcg gattcgtgag acgaattcaa gttttaagtt caagccgtaa
     7981 atgtcacggt cgaaatcgag caggtaagct tcaaggctgg gcgtctgccg atcgggtgtg
     8041 aaggttgggt tgaagccaat gttggtggca gccatgaatt tttctgtgcc aaggtatgcc
     8101 caacaggcgt aaatgccatt ggatggggca accttttgct gcacataatc tacattggcg
     8161 gtgggaaagt tgatggtacg tccgcgcccg gcgccgcgga tgacctctcc gctgagggca
     8221 tagggacggc caagcaattt tgcggcttcg gtaacattcc ccgtggaaac gagttttcga
     8281 atctctgttg aagagatcac tccgctttca tcgctgagcg cgttgataat ttccactgtg
     8341 tatcccaact cattgccgat ctcgctcagg cgtgaggcat ttccttcgcg ccccctgcca
     8401 agggcaaagt cataaccgat gagcaggtgt tcgaggccaa gactctgctt cagttgagtc
     8461 atatattctt tggcagaggc agttgaaagg tcccgtgtga agcgttgagt aatgacatga
     8521 tccacgccca tcgccatcaa aagatcagcg cgttcttcgg gtgtggtcag gcatttaatg
     8581 tcacgcccgc cgagtactgc ggcagggtgc ggggcaaagg tcagcacaac agcgggctga
     8641 ccttgtgcat gggcttgctg taccagtttg tggatgatct gctggtgtcc gcgatgcacg
     8701 ccatcgaaca caccaatggt gagccatgcg ttttgtatgg atacttcttc aagggagcga
     8761 taatgagaca tgatataaaa aagccgccgg ggcggcggct ggtgtcattg caagggtatt
     8821 ccatttctgt gttgaatgaa ataccctctt cgcaatgacc ctatttatta atcggaagta
     8881 aagaaaacct ttttgggctg ccattcgtgc gagccatctt cagttgtgat cagttccatc
     8941 agggcaacca actcaccctg tgtactgaca ccgcgaacct tcgtttcctt gggttcaccg
     9001 acaactttga cacggtgccc atggcggacg ccttcaactt catcaggatt aagctccacg
     9061 gcaggccaat caccaagcgc ttctgcggcg gggatcagat attgatacca gtttccggcg
     9121 gtgaaggctt cctgtaattt gcgcaggggc acggaatcgc gcagggagaa acgtccgctc
     9181 ttggtgcgac gcaggccaac aaggtaggcg ccgcatccga gcatgactcc aagatcattg
     9241 gccagtgagc ggacataggt gccggaagag caatgcacat caatcaccac ttcgggggga
     9301 gtccattcaa gcacttcgag atgatggacg gtgatcttac gcggggccag atcaacttct
     9361 tcaccctgac gcgccatttc ataggctttg cgtccctgca cttttacagc agagtagggt
     9421 ggcggggttt gttcgatctc accaataaat gttttcaggg cttcttcaaa ttgtgtttcg
     9481 gtaacattga ccgggtcttt tgtaggtgtg accttgcctt cagcatcgaa ggtgtcggtg
     9541 gaaccgccca gacgaatgat ggcttgataa cgtttatcag aagcagatac atattcgctc
     9601 aaacgcacag ccgggccaac aagaatgacc agtacgccgg aagcacgtgg atccaatgtg
     9661 cccgtatgac ctgcacgacg cagaccagtt ccattacgga tcgcctgcac cacatcatga
     9721 gaggtcatac caacaggctt atctacaaca agagcgccag atatggcatt ttttacatcc
     9781 tgactattca tcttgcattc ctccttctaa tattctccag ttaattatga cacatttgtc
     9841 caattttaca acgattactg tggtcatgga tttttttatt ccacttacag atttaacatt
     9901 tcaaaggttt tcgcaagaac ttccttttga acatcctgca aattaccttc aatgtctgca
     9961 cctgctgcag cggcatgccc accacctttg aagtgtttgg cgatctgcga tacatcaaga
    10021 cctgattcca gtgcgcgcca tgaaattttc acatggtgat cgctttgctc tacaaaaacc
    10081 attcccactt tgtgagaatc gattgcagag atcatattga tcaggtcggc atcatcattg
    10141 ccgccatatc cggctgattt acgatcttca agggtcaatg tgccccacac tacaccattt
    10201 ctgctttcca aactggagag accggcaccc caatagcgtg cggcaggcag ggacttgttc
    10261 accagagcac gcatgtaaat ctcgggcata tccacgccgg attccatcaa ggtggctgcc
    10321 tgtcggaggg catccggggt ggtattggag gtgcggaacc caagggtatc ggtgatgatg
    10381 ccggtcagca aagcagcggc taccgctttg gtgatcttca aaccccacac aggcaaatgc
    10441 ttggtgagga tggcggcagt ggcaacttca tccgcttcga tcaggttgag ctttccaaat
    10501 ttttcgttgg tcttgtgatg gtcaatattg atatcgggct ggccgaaatt ctcaaagatc
    10561 ttgccagtac gtttgaaatc ggcgcaatcc actgtgatga aggtgtcatg gttcccctga
    10621 ggttccttca cgatcaactg gcttccttcc aggtatttga atgaggcagg cgcgccatca
    10681 acaaggacca tttgcacgga tttccccgct tcacgcagag ccaatcccag tccaagcagc
    10741 gagccaattg catccccatc cggtcgaacg tgcgacacga tcacgatctc ttttgccgca
    10801 cttaatctat tttttatctc ccctgaaatt tgattgtcca cttatgctta tcctccaggc
    10861 acttaaaaaa taaggggtgc atttgaacgc accccttgta aattacattt atttcttctc
    10921 tcttaaccct gccagaactt tttcgatatg gtctgcgtgt tccggagttg ggtcccaatg
    10981 aaaccgcaac cttgggaaag cccgcacttc cacacgggct gaaagggtgc gcctgagaaa
    11041 accagaggca gattccagac cttcaagtat ctcagccgag cgccctgcgc cctcaatggc
    11101 cgatacatag atatcggcat aagccagttc ccggtcgatc gtcacatcgg tgacgaagat
    11161 ctggtgtaaa cgcgggtcat taacttcacg aattagcagt tccgacaatt cctgccggac
    11221 acgatctgca atacgctgta aacgaattcc tgatggcata aagcgcctca cattcatcgc
    11281 tgcaggcgtg tttaacgtcc atacaacgaa tcacatatat tacgaatttt caacaatata
    11341 acaaacaagc tggtcgccgg gcagaatatc attaaagctc ttgaaaccga cgccgcattc
    11401 ataaccctga cggatctctt tcacatcttc cttttcatgg cggagcgaag agagatcgcc
    11461 ttcatagatc atatcagtac cgcggaagag gcggacacgc gcgccgcgtt tgagttcacc
    11521 atctgtcacc ttgcagcctg ccaccttgcc gaacttcgat gcagaaaata ccgccagaac
    11581 ctgtgcccgc ccaatgacct tttcaaccag tttcggttcg agcatgcctt tgagagcctt
    11641 ctcaatatct tcggtcatgc ggtaaatgat ctcgtacagg cggatatcca caccttcttt
    11701 ttcggccaga cggcggccat ccacatcggc ttgtacattg aaaccaatga tgatggctcg
    11761 cgaagccgcc gcaagcataa catcgttatt gctgatatta ccggtttccg catgcaggat
    11821 gtgaagaccg atctcacctt ccccgagctt gttcaactcg ccgatgatcg gatccagcga
    11881 cccttgcacg tcggctttga taatgaggtt gagtcccttg gcttcacctg cctgaacatt
    11941 ggcaaacagg tcttcgagtg aaaccttttt cctggtttgt gaaagggttt ttgcggcttc
    12001 cacacgctcg ttcacaatat tgcgggcttc tttttcagaa tctacaactt caaatatatc
    12061 accagcggaa ggcatgccgc ttaaacccat cacagccacc ggcgtagatg gaccggcttt
    12121 cttgatcggc ttgcccttgt aatcggtaat ggcgcgcaat tttccgtagg cggtccctgc
    12181 tacaacgatg tcactagctt caagggtccc gttttgaacg agcaatgtcg ccagcacgcc
    12241 tttgctttta tcaagctcgg cttcgatgac tgtgccgata acttttccat tggggttggc
    12301 tttaatctcg ctgctatctg ctacaagcag gatgccttcg agcaaatcat caataccctg
    12361 tttctgtttt gccgaaacat ggatcaccat ggtattcccg ccccattcat ccgggacaag
    12421 ttcaagttca gcaagttgtt gtttaacacg gtcaggattg gcgttcgctt tatccacctt
    12481 gttcaatgca accatcaacg gcacccgcgc agccttggca tgggcaatgg cttcacgggt
    12541 ctgtggcatc acaccatcgt ccgccgcaac gaccaacaca acaatgtccg caccctgggc
    12601 gccgcgggca cgcatttggg taaatgcggc atggcccggt gtatccagga aggtgatcaa
    12661 acgtcccttc ttttcaactt gatatgcacc gatatgctgt gtaatgccgc ctgcttcgcc
    12721 tgccgccact tcggttgacc gaatggcatc caacaaactg gtctttccgt gatcaacatg
    12781 cccaagaatg gttacaacag gcgggcgggg cttcaaaaat gcattgtttt ccccggcaat
    12841 catctgacgc cagagaggca cttcccccac ttccgctttg ctttcctctt cttcaactgt
    12901 ttcgagaacc gcttcaaacc cgtattcacc aaccacgatc gcagccgtat caaaatccac
    12961 ggtttgatta atggttgcca tcacgccatt tgacattaat tttttgatca gatcaatcgg
    13021 gcttttctcg atcttcagcg ccaggtcacg aatcacaatg ctggcaggta actcaagttt
    13081 atttccgttg ctgctcatag gccacctcca tctaactagt atgcggatta caacttacag
    13141 aatccgcgaa attctcaatt taccagcgag aggcccgtga gcgagtggct attcgttcac
    13201 atgactttac ttgccggcct ctgcctcaga aagaggcaga gagttcataa attcttccag
    13261 tcgagcgcgg tcatccgcag tcaactcggc tttgagcgcg tgtcccaaag ccccccttag
    13321 cccccgcacc caacattcac gttgatcgtg caggtatgct cccctcccgg cggctttgcc
    13381 ggtcgggtca acccaaaccc cattgtccgt acgtacgatg cgcaccatct gcctttttgg
    13441 caaaacttcg cgacaaccca cgcaagtccg ctgggggata tgctttacac gttgggcagg
    13501 ttttttggcc accttcaaga tacctgaaac tattcccaat caccttcaac gtcgccgccg
    13561 cgtttgtgaa tcttacggcc gaccacggtt cccaaacctt catcgaactc caattcaacg
    13621 gctttcttct tgcccttctt gctcttcttc ttatcgccgc tgacttcctc ttcggcaggg
    13681 cgcgcaccct ggaagatctc gggcttcatc ttgaacaact catcaagaga cacgccatcc
    13741 ttcgcatgct cttcatcttc ttcaacttca actggacgct tcgcatcgcg cttcccttcg
    13801 gcctgcttct tcacaaccgg cgtttcctcc gccgctgcgg caggttctac aggagcagtc
    13861 tcgacaacga cgggttcggc agccacaaca gcaacgggag cctcttccac cttttcaggt
    13921 tcggggaagg tcaatgccgc cagcgactct tcaatgtttt gcatggcctt cgagccaata
    13981 cccggcaaac caagcacctt gttgggttca gtcttgagaa caaacatcaa ctgaccaact
    14041 gtttcaaaat tggcttcggt caaaatattg aaaatatgct ctttcatgcc agcctggtcg
    14101 agcggcatct ggaaggcggc ggcagggata tccgcgcggg cggctacagt gcgttcctct
    14161 tccacacggg cagcttcttc cttgatctgg atcgtgcgtc gttctacgcg gtcaatgaat
    14221 tgacccagta ccgtatattc ttccggagta attgcacggc cttcggcctt cttggcaagg
    14281 acttcctgca cttgaggaat ggcttccatg gcggcaggca acattgcgcc aagctcggca
    14341 ttagcctgca atttatcgag ggtatcactg gcagcctcgg tcaaagactt gatgtcaata
    14401 cgccagccgg tcaattttgc agccaggcgg gcattctgac catcacggcc gatggcaagg
    14461 cttaactggt cttcacccac caccacagtt gcagtgcggg cgccttcagt atccacaagg
    14521 tacacgccat ttacgcgtgc cggactgatg gctttggaaa tataggtaat gggatcctga
    14581 tcccattgga tgatatcgat cttctcatcg tgcaattcct tgacgatggc ctgaatacgc
    14641 acacccttga tgcccacaca tgcacccacc gggtcaatac cgggttgtgt ggcagaaacc
    14701 gccacctttg cacgcgcacc tggttcacgc gcaatggcgc ggatctcaac aatgccatga
    14761 taaatttcag gaacttcatt ttcaagcaag cggcgcagga aattacgatg cgcacgagac
    14821 agaatgatct gtggtccgcg tgtgccatcc ttgacttcca taacaaccgc gcggatacgg
    14881 tcatgcagtt tgaggcgttc ccccggaatc ttctggttat tgggcagaat accttcggct
    14941 ttcatatcca gaccgatggt tgcgctctgc gcattgctgg cctgaacgag accggaaacg
    15001 atctcaccga cctggcgctc gaaatactgc atttgattcg aacgttccgc ctcgcggata
    15061 cgctgctgaa tgacctggcg tgcagtctgt gcagcgacac ggccaaaatc tgccggggtg
    15121 gtttcaacta aaaccatatc acccacctgg gtatctggct ttaccttttg agcttcaccc
    15181 aacaacacct cagtgcgcgc atccacaacc gaatcttcga ccacttcctt ctcagcgtaa
    15241 acggtcactt cgccggtgtc agggtcaagt ttggcttcta catgctgcgc agtcgaagcg
    15301 ctgacagcgc gccgatacgc ggaaaccatt gccgattcga ttgcggcaat caccacatcc
    15361 ttggcaagtt gtttttcttc cagcacttca ttgaatgcca atgcaaattc gtttttagcc
    15421 atgctgctta ctccgttaac tcctgttcct tacaagcaaa aaagtggggg taacccactt
    15481 ttcgatgtct tctctattgg gttttccgtc cgacgaaatt ttagcataga ggtatggaag
    15541 gcgcaaggtg tcaacataaa tgaatctact ccttgacatc atcgcatttc tgcgtaaaat
    15601 accgcacaac atgtatacat ccattcgaaa taaaaggccg caccatcact ttttcggaac
    15661 agatgtgttt ttatttttgt gaacgagaga gtcgcttgag cggctctctt tttttatatc
    15721 cacacaccca aaggagagaa tatgtccgca gaaacaaaac ccgccgtcat cggtttccta
    15781 cccgatgaca cgccgcccat cggcgcaatg ctcagccttg gtttccagca attcctcaca
    15841 atgtttcctg ccactgtcct ggtggctatt ctgaccaagt tcgatgtcgg cattaccttg
    15901 ctggcctcgg gtcttggaac catcattgcc ctgctggttt caaagcgtaa gatccccatg
    15961 tactacggct catcgttctc ctacatcgca gtcatcatta cagtgatgaa caagttcgct
    16021 cccgattgtt acaactctgc tgccacatac tgcccggatg gagtacgtat tgtgcaggtg
    16081 ggcatcattc tgaccgctgt atttgaaatc ctcattggcc tgctcatcat gcgaatcgga
    16141 aaagccgctc ttgatcgtgt gctccctccc atcgtcacag gctcaatggc catggtgatc
    16201 ggtattgcat tggcaaatgc cgcgttgggc aatgccggta actgggccgc ccccgaactc
    16261 gccgctacaa catggactgt tgcttttatt acgctgatcg ccaccgttct tttttctgta
    16321 tacctgcaag gcaagggatt gatcgggatg ctgccgattc ttttcggcgc gatctttggt
    16381 tatctcgtgg ccattccatt cggtttggtc catttcgaga atgttgccag cgccagttgg
    16441 ctggcaatgc ccaaattcac cctgcccgct ttcgaaaacc cggaagcctg gggaatggct
    16501 cttagcattg cattgatctt cattgccact gttcccgaat ccacggcaca tctttaccag
    16561 atgagcctgt acattgacga acttgctgcg gagatgaaac gcaagcctta taacatcaag
    16621 gaactcatcg gcctcaatct cgttgctgac ggatcggatg acatcgttgt tggcctgctt
    16681 ggcggatgcg ccggtacaaa ctacggtgaa aacaattcac tgatggctat cacccgcaat
    16741 tattccactg cagtattgat gacagcgggg gccatggcca tggtctttgg tttctttggc
    16801 aaactggtgg cgttggtcaa caccatgcct gccgctgtgg tcggtggatt gagcatttat
    16861 ctatttggcg tcattggaat gcagggtgtg gcgctcattc aatcggagaa ggtcaatctc
    16921 tttgacccga aacaacttgc ggtcggcgcc attattttga tctgcggcat cggtggcaat
    16981 cttgccctgc ccaatggcgt gttcccattc aacgtgcctt tccttttccc gaatggcatc
    17041 cctgcgattg ttttcgcggc cattgtcggc attttcatca acaccatttt cctgatcttc
    17101 aagactccag aagtgcgtgg aggataggtc tcggaatgtc aaccaggcaa acaggtacac
    17161 atttgtacct gtttgcctat ctcatcttcg cgtgttttcc tttatacttt tctcatgatc
    17221 aaactccctg gtttaattga cccccatgtt catctgcgcg aacccggcgc aacccacaag
    17281 gaagattttg attcgggtac ttctgccgcg ctggctggcg gtgtgaccat tgtgctggcc
    17341 atgccaaata caaaaccgcc catcttcgat gcagccacac tggaccttgc tctggacgcc
    17401 gcccgtcaaa aggctcgctg tgattacggt caatatgtcg gcgctgggcc tgataactcg
    17461 gactggatca gccacaaggc ggagtctgac cgtgcggcgg gtttgaaaat gtatcttgat
    17521 tcaaccttcg gcgaattgcg tctcgatgac atgtcgctct ggactccgca ctttgcaaat
    17581 tggccaaagg aaatgccgat tgtggcgcat tcagagagcc gcagtatggc cgccgcgatt
    17641 ctattcgccg cgatccatga ccgccccgta catattgcgc acatttcgtt aaaagaggaa
    17701 gttctgctca tcaaggctgc caaggaacac ggcctcaaag tgacctgcga agtctgcccg
    17761 catcacctgt tcctttcgaa ggatgatatc cccgccattt cgcatgggca ccccggtcgt
    17821 ggagaagtgc gtccgcgcct tgctacaaaa gcagatgtgg aagccctgtg ggcaaatatg
    17881 gatgtaattg attgctttgc aaccgatcat gccccgcata ccattgctga aaaagactca
    17941 gataacccgc cgcccggctt tcccggacta gagacctttc ttcctttaat actcaccgcc
    18001 gtacaggatg gccgcttaac aatggatgat gttatttcaa agatgtatac aaacacaaaa
    18061 cacatcttca acctgcctga acagcccgaa acctgggtgg aagtggatga gaatgcccaa
    18121 tatgagatca aggcggctga acagtttacc cgctgccatt ggactccttt tgagggttgg
    18181 aaggtgaaag gccgtgttcg taaagttgtt ttgcgtggaa aaacggcctt tgaagatggg
    18241 aagatcctgg ctgagcctgg ttacggaaaa aatattcgat ccttcaattg aaccgaatca
    18301 taatgccaca ctagagcgga tcctttaacg ggtccgctct ttttattacc ttattgttat
    18361 tttcagactg actttttatt caaagaccct agtaccaata tcacatccct ttcccctcta
    18421 tttagagagg ttacaaactt tcgatccgtt tatagaattt cagtcacaat caaagaattg
    18481 taatcatcat aataaaggag taagtatatg aataagaaag cttttatcgt tttgcttttg
    18541 gcagccattg tgctggccca attcagcctg gcttccgccg ctgctgtcag cggggatgca
    18601 tccattacca gtgttcccac cttccttcca cttaaacaga accttgatgt ggaatgggct
    18661 gtcagcggcc tgcctgcagg gggaccccgc taccttttaa tgtgggcacg cagggaacca
    18721 tcaacaactt acgacatggc tgaatgtaaa gtggttgaaa accttaccgg tcttactgaa
    18781 gccagtggag cttttgaata tagctttgcc ggtaccaaca tcgctttctt aatgatcgat
    18841 ggcgacgtca tcgaaaccac cctgaccctt gacaccgacg attgcgcggg cacgccagac
    18901 ctgccagcag atagttcagt catgggcagc accaccatcg acaacaaagt cccatcgatg
    18961 agggcaggca acccgccctc actttccgct gccaacatca tgcttcaacc tgttgcttgt
    19021 aacacctttg aaatgtgggc ggttgcctct gatattgcag gccttgatcc cgatgcacca
    19081 tactcagggc ttgtttcctg ggggcaaaca accaacggca cctttacccc cgccgcccca
    19141 attggcaagg ctgaagatac cctttcgtgg gtatacacct tcccagccac agctaccggc
    19201 cagtggacgt tttacatcca cccgaaagat gccgccggaa acagcgggct tttgggaaac
    19261 atctaccgcc tcacactccc cattgaacca caggaattgg aggactgcgc cacattcagc
    19321 gatgtcagtg gccatgcgga tgaggtttac atccgttacc ttgcgaccct tggcctgatc
    19381 aacggcttcg ccgatggaag cttcggcccc gataacaccc tcacccgtgc tgaagccgcc
    19441 acccttttcg aaatctccaa cggctacgat gaaaacaccc tgcccgcttc tgccccggct
    19501 ggttgtgaat tctccgatgt cacggcttct gattggttcg ctggctgggt ctggcaggcc
    19561 tgtgcagacg gcttcatgaa cggtgtcggc ggcggtctct tcgaccccaa caacctgctc
    19621 acccgcggtc agatcgtcac catcatgaac aatgttgcca attccacacc tgccggtgga
    19681 tatttgaata ccatcagtgt tgtaaatagc ctttggaatc attttatttt caagccgatc
    19741 ctcaaagcag ctttcacaga tgtccctgtc ggcgcatatt atgccgatcc cgtgattcaa
    19801 gcctacggtt ggggggttgc cgatgccaca tctgaaacca ctttctcgcc cgatcaaccc
    19861 gtcactcgcg gtgaatttgc caagatgctc tatcgcgcct tgagccgcgt cgggtaattc
    19921 aagaccgaat catcttcacg caagtcacat ttcgttttgg gtggacccat atatgggtcc
    19981 accc
//
LOCUS       DAOQLC010000080        19707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_961_length_19707_cov_20.797680, whole genome shotgun sequence.
ACCESSION   DAOQLC010000080 DAOQLC010000000
VERSION     DAOQLC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19707
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_19707_cov_20.797680"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..>1367
                     /gene="trmFO"
                     /locus_tag="PL000_13975"
     CDS             55..>1367
                     /gene="trmFO"
                     /locus_tag="PL000_13975"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNC90058.1"
                     /translation="MTSLTIIGGGLAGSEAAWQVAQRGFKVRLYEMRPINPTGAHLTG
                     DLAELVCSNSLGSNLPDRASGVLKNELRLLNSMLLECAEASALPAGAALAVDREAFAR
                     LVTEKIQSHPNIEVIRAEMKEIPDSPVIIASGPLTSESLSRSIAKLSGDEHLFFFDAI
                     APIVLAESINMEIAFRASRYGQGDQDEGDYINCPFTKDEYYAFVNALQTAERIQLHSF
                     EDAIRTGVKAGQFFEGCLPIEIIAERGTDSLAYGPMRPVGLRDPRTGKRPYAVVQLRQ
                     DNLAGSLYNLVGFQTNLKFPEQRRVLRMIPGLEQAEFERYGQMHRNTFIASPKLLRPT
                     LQHITRDDLFFAGQITGVEGYMGNIATGLLAGLNAAQLLQGKPPVTLPPETMLGALCH
                     YITHADLKDFQPMKANFGILPPLELEKRIGKRERGQRYADRAANAL"
     assembly_gap    1368..1467
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1468..2346
                     /locus_tag="PL000_13980"
     CDS             <1468..2346
                     /locus_tag="PL000_13980"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNC90059.1"
                     /translation="YLSSIFALLALVAGWYIVSFINREPDPVSFDSSRAYADVQTQVD
                     FGPRIPGTDGHAKIRAWMQAELESAGWTVEIHETERMGHPVYNLIAKRGEADPQIILG
                     AHYDTRMIADHDPQPTNLNKPVPGANDGASGVAVLLELARTLPEDSVPVWLVFFDTED
                     NGRIEGWDWILGSRAFAEEIPVRPEAVVIVDMIGDADLNIYLEQNSDIAIRTEIWSIA
                     AQLGYGDKFINEEKYSMLDDHTPFLEAGIPAVDIIDFDYPYWHTTQDTPDKVSAESLG
                     AVGNTLLTWISGKTSK"
     gene            2425..3705
                     /locus_tag="PL000_13985"
     CDS             2425..3705
                     /locus_tag="PL000_13985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC90060.1"
                     /translation="MTVQESLQKIRTPWVERISRELASGEGVRVGFIEQLERFYELLE
                     HTVVTGNLAWLDPVLYDWGQSPTETNLEQGDYYVSFIVNRMTTLTIEVARENLGKEDA
                     LELVAAVVPIFTHSLSVVVRYEMETRVAHISSELNKTQEKLQQLDHNKSNFISVAAHE
                     LKTPLTLIEGYTTMMVDMVEQSGQSPIDSLLKGVNTGIQRLRQIIDDMIDVSLIDNNL
                     LALNFQPLWVSHMLNLLKNELKKTVTDRKQTLIVNQFEGSDLMIYGDSERLYQALYNV
                     ASNAIKYTPDHGTITIEGRLLPGFIEITIKDTGIGISPENQSRIFEKFGQLGRTDLHS
                     SGKTKFKGGGPGLGLPITRGIIEAHGGTIWVESEGYDEEKLKGSTFHILLPTRTEATD
                     PLITKFFGAPGQLKSESEKETIGKETPPTNNPTS"
     gene            3665..5029
                     /gene="hflX"
                     /locus_tag="PL000_13990"
     CDS             3665..5029
                     /gene="hflX"
                     /locus_tag="PL000_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC90061.1"
                     /translation="MAKKLPQPTTPPRERAFLVGVEIRGDKSMLPLNDSLAELALLAD
                     TAGVDVVGELTQRLDRPHVVTYIGPGKVEELKALAEETLTDVIIFDDELSPRHQRELE
                     KALSIRVLDRTALILDIFAQHAHTSEGMLQVELAQYEYNLPRLTRAWTHLERQAGGGG
                     GRAGSTGGVGLRGPGETQLEVDKRAIRKRITHIKRELEKVEAHRMRYRAQRKRSRIPT
                     VALVGYTNAGKSTLLNRIAKSEVYVADQLFATLDPTTRRVQLPGDDQALMTDTVGFIQ
                     KLPTSLIAAFHATLEEIAEADLLLHVVDISHPNALNQYQAVQETLNEIDAGHIPMITA
                     LNKADLLNHPENAIEVLKAFPKAVSISAREGTGIKDLLHLIREELYESYSPINVRLPY
                     QQGALISLFHELGQVERVEHERGGVVMQGRIPGRLLAQYASWHIKPNQQEMTENEADD
                     LEEE"
     gene            5029..5229
                     /locus_tag="PL000_13995"
     CDS             5029..5229
                     /locus_tag="PL000_13995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90062.1"
                     /translation="MSKFLDRMSEFLAHRKGLLPIIGLVLILVNLLVQFIFPGGFLAS
                     TNLFLHIGLLVAIFGLMLAWAL"
     gene            complement(5234..6253)
                     /locus_tag="PL000_14000"
     CDS             complement(5234..6253)
                     /locus_tag="PL000_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC90063.1"
                     /translation="MNTTSSSVTLRWVDLIRVIGAFLVVMAHVAYTGAGPVLASTYYF
                     VISRVAVPLFFMVSGFLLLKKEEPYGVFFRKRAVKVAVPFLVWSVIYLLWKREGFETG
                     FSLDLVVSYVVKIVRGPRENHLWFFYALIGLYLFTPILRVFVARASKTDLFYFCGLWF
                     LVVPIFSLLHEFTPIKIGFELYFVAGYTGYFLLGHVLGLFEFSRKQLYGLAVLFLFLS
                     VLTAYLDLQSNLLKWNTQYFVSYLSVNIVLLTVAAFILLREIPIDDRWYAFLAPLGRA
                     SFGIYLVHVIVLTELEKIPAIHYWFSAGSSVYMIPLLGLFGFAVSYLIVAVVQKIPVL
                     RWAMP"
     gene            6335..8314
                     /locus_tag="PL000_14005"
     CDS             6335..8314
                     /locus_tag="PL000_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC90064.1"
                     /translation="MPFLSPGQTLLNQYQIETFVTLTPLGELYRATDTRSNKPLALTL
                     LPKTISENIEKLKHLEAEAARLRSISHPNIVPFLGLYQTPTTNFLLEEWVNGPSLVEI
                     LARPPLSATEALVYTKAICAALEALHKQNYLHLNLIPEFIHINKRGEILVSGISAAQQ
                     INAIIEYPHGKYLRLYASPEQFTDQKLTPASDIYSLAAILYTLITGAWINGKSAPKTN
                     AEIKKAHLDLTPPAPITLNKEIPDHFSRMILWALRKNPENRLKTTTELLSSLALATQS
                     SVDQVPLRTDPKTAPVTSAVLEGWNFLPPPKPNIITEDSLPLEERMTAIAQPKKRRSG
                     MGIMTAFVVVLLLGFASLFWLVRPAPVVIPTPIQFTPFAADFTPPPTFTPEPRPTDPH
                     GGRIAFTCTRGDYNQVCIVNRDGSGYTQLSDMDASSYYPAFTPDGSSLLFASNRNGAF
                     DLYLLQFGQKQLTQLTQNIGNVVSPDYSADGQKIIFVNQVGDSLPAIWMVNADGLNPH
                     LVYAGVNEIVAVTWSPDMSQIAYAMSTGAPLEYEIYVMNSDGREHRQVSRGLQGIGGS
                     LDWSPDGKSLLIYAGPFGDKDIFSIDIASGNYTQLTDGGNNAGASYSPDGLYIVFNSL
                     RNDEQADLYIMRVDGSNQIQLTNHPEPDWGPRWVE"
     gene            8618..9205
                     /locus_tag="PL000_14010"
     CDS             8618..9205
                     /locus_tag="PL000_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HNC90065.1"
                     /translation="MSKQPSSYLSSKLEGRPKADGSGNGIYALEPINKGELVAVFGGV
                     VYEWDAFIHLPDRERSLCIQVEDRHFLVPRPIGEGDYVNHSCNPNAGLSGQIGLVAMR
                     DIKIGEEVCFDYAMSDTMPYDEFECGCGAATCRGKVGGNDWQKPELQKRYAGFFAPHV
                     QRKIDAQRKEKRAFEQAIRMAKSPKFPSVTTPVYE"
     gene            complement(9248..10099)
                     /locus_tag="PL000_14015"
     CDS             complement(9248..10099)
                     /locus_tag="PL000_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90066.1"
                     /translation="MSQINLKKLNTHRTRTFNLPPNKRLSTPAQALTFIKKRGFVYFW
                     PIKGIDLPSLWAAVAGDRPVAEKHDDPGHITWGWKDNALDKKVWYYAKILRGKATMIS
                     LETAPYFYALSENYGSPDEDYLIAYQEGRLTQASKQVYETLLENGALNTLDLRKMAKL
                     SNAKDSEFNKALEQLQRDFKILPVGVAQAGAWKYSHIYEIVPRHFPDLPEQARQITES
                     QARMKLLELYFNLVGAAQMRDVVKLFGWSTEITNRIVSRMTDQGLLAKSTHPKHVGEW
                     LALPSLV"
     gene            10178..10528
                     /locus_tag="PL000_14020"
     CDS             10178..10528
                     /locus_tag="PL000_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90067.1"
                     /translation="MPTLQTVYIMAFTPEKMDNNTLISVCRMAADMLSAKDSTFAQRM
                     GMIAKTTRMTFSNQLYVSTMHTPQTMQTTLGGWVKNENMPFAPVLGDTFFPHGMRDPK
                     GRENFFLFYFHITN"
     gene            10730..11785
                     /locus_tag="PL000_14025"
     CDS             10730..11785
                     /locus_tag="PL000_14025"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HNC90068.1"
                     /translation="MKHTTLIALLVISLFATACGAAPATAEPTPIPTVLADNTIIAEG
                     NLEPIHYTELALNASGLVSEVLFSEGDQVAAGDVIARLKSNEAQTLETAQAKALAEMT
                     AAYQEVRDAQFKLDNFDVPSDFDGMTPSEAVSEMLVKLDKARADFEPYKNLSERSLAQ
                     TEAEKNSGVVTGTAKIYKQALDDAWARYRKAILWLELESTLQNANSRLTLAQKDYEAL
                     QDPSFSADTAGARAALASAEVRAPFPGLITDLNLKVGEFAASGQPVITIADNSKWVVK
                     TTDLTEIDVVKIKEGQSVTVTLDALPEVELSGTVLYISQDFSENQGDVVYEVTILLTE
                     QDPAMRWGMTAIVKFVE"
     gene            11899..13032
                     /locus_tag="PL000_14030"
     CDS             11899..13032
                     /locus_tag="PL000_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90069.1"
                     /translation="MAFFQKNNHTSTDTRPMIDLRDVNKFYKTAIGDYQALNNIDLQI
                     NAGEFVSIIGKSGSGKTTLLNMITGIDIPTNGEVWVNDTAVHKLSENKMARWRGKNLG
                     VVFQFFQLLPMISVIENIMLPMDFCRTYPMRERHQRAMQLLELVELADHAHKLPTALS
                     GGQQQRVAIARALANDPPVIIADEPTGNLDSKTAESVFSLFNDLVSKGKTIIIVTHDS
                     ALAKRTHRTALIADGEIVNEYVAKAMPTLTAAQLLQLTRSAKTLQFEPGAMILSEGTH
                     ADTFYVVSKGTVEVVLPRTNQSDVVALQLGPGKCFGEIEFFHEKKHLASVRASESGPV
                     EVVSIEYGQLKELLDQSQVTRDALHQMADKHEDEHMSIRGGSK"
     gene            13029..15419
                     /locus_tag="PL000_14035"
     CDS             13029..15419
                     /locus_tag="PL000_14035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90070.1"
                     /translation="MSSRWKKVWADFWGNRGRTFLTIMTIMVGTFAVGFNSNMGAYMM
                     QSMDDDYLSANPAEAQVYAGPLDDDMVKIARTVPGVEQVQGFSSVGARIVRPDGTYTN
                     VNFIALENPGKLTLNLLKPVTGEISIPTYGKNETIFDGSAMGMGYKAGDTILVELADG
                     KRRELKLTGYMHDVTGIPFNLANTVGAYVTPDTLEWLGGDTSSYEMLAISVLEKQTDQ
                     EHVTTVAQAVADRVERAGATVFFVNVYEPGHHFAWSITQGVFFVLSILGYLTVLLSGF
                     LIINTITALMTQQTRQIGIMKAVGGGTIQITGMYVVLILGFGLGALAIAIPLANLAAE
                     EIGGGMAQWLNFYASPYKGYTSTLIQQVIVALVVPLLAAILPIYNSVRVTVREAISDY
                     GLGGNARPKADSVNQKSVLIPRPIRISIRNTFRRKVRLGITLFTLVLAGAVFIAVYNL
                     WASFDKVMQDIQGYFLADINIAFDRGYRFDKVASITENIPGVEGAEGWLEYPGTLKMG
                     DDESGTQILFVAPPSTSTLISPIISTGRWLQPGDENAVVIGNHLLKVFPDLKIGDWLT
                     IETNGKETDWQIVGTYSLPGNVSPPLLYANYEYLSQLVGEIDMAYSLRITTTQHDSVT
                     QKRINDMVQAAFEAQGVQIRSTQLSADFIRDQKAQTDILVYFMLVMATLIAIVGGLGL
                     MGTMSINVLERTREIGVMRAIGASNGDIQAIVIVEGMVIGIVSWLISIVIAIPITNIL
                     CYGVGMAILTAPMPAVYGVSGIAAWLIFTIVLGSLASALPARRASRLTVRDTLAYE"
     gene            complement(15512..17467)
                     /locus_tag="PL000_14040"
     CDS             complement(15512..17467)
                     /locus_tag="PL000_14040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90071.1"
                     /translation="MDEKTVPIGNLPPHLMALLNKQKKVVDWGEDIHYRALFDQTGEV
                     VFIIGMDLHYITANRQALDLLGYKKEEIVGLPVSSVMSLSDGVEDGTVLDDDTNMVER
                     ILKRKDGSTFPVEVSASIIYNERNEPAYIQSIARDISERKKSELALTRYSRILSAINH
                     ATAQLLRSSKIGEMIPDALAALGEAIGVTGCAIFEVNTFSPQPAVLTQYQWIKNDTPD
                     LNVSDLILPIIPSILKASGGQFFNNDVGRHGPSFFVFPIYGTLDSWKFLGVFDTSKLL
                     AWSSSECDAVEMAAGLMGSALQRTSYEETIRENENRNRIILSVLPDLLIRLTTDGVIL
                     DYTTNPDHPLYLHRDMISGRRLSDIWPEETVQSILTVPNVDEDDEEQRPVFGFTLPFS
                     ARDYESRLLPISETEVLVVIRDVTDQARLDRMKSDFINRASHELRTPLTTALLMTELI
                     QDGGSREELDQYWRTLTSELNRQKVLIDRLLIAGRLESGMMELESTLLDLTPVLEESI
                     MAVRPIGNKRDVSIKLMSEQSDFYIAGDKSGLQQVFINLINNAIKFSPKGSTVLVGLW
                     KGNGYVHISIADQGIGIPPDAMPHLFQRFYRARNVTIAEIPGSGIGLYIVKSIVEKLG
                     GDISVESAPQNGTTFTVSFKDAHAPQV"
     gene            complement(17460..18596)
                     /locus_tag="PL000_14045"
     CDS             complement(17460..18596)
                     /locus_tag="PL000_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HNC90072.1"
                     /translation="MNSKPLILIIDDDPALLMGVSATIKKYGFQVVSAEDGNDGLAKA
                     KEIKPDLILSDVMMPPPDGLELRRIMSQDPQLASIPFIFLTARSGVEDKVSAIMDGAD
                     DYITKPFVVYELIARISAVLRRVKIAEENGREQARVAAEADLERVRQEILQNFHHEIR
                     TPLSNIMMPLQLAINNKFDDAQEQSRFLSKALSSASRLESLTTDLILLSNIDQGNLNT
                     IRQPIDMEMDIASIVKRRLDRYDEKGLDFTMDISSSGKISAPRREFTHALVHLVDNAF
                     KFTSKNGKVHLRITPTGNGGATLDVFDNGPGIPSRLHEKVFERFYQISQGDAREFEGM
                     GVGLSIARAVFRSLRGEVKILESLRGCHIQAHLPDPQPGDVTYG"
     gene            complement(18599..18973)
                     /locus_tag="PL000_14050"
     CDS             complement(18599..18973)
                     /locus_tag="PL000_14050"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC90073.1"
                     /translation="MTQEEDNNLIPSREQNVFLIINRQMIPLEKKVTRLGRQLGNDVV
                     LQEESVSRFHAEIRYEDGKFILYDNNSTAGTYVNSKRIDRCVLISSDLISFSCIQVMF
                     VNNNAKLAGKAMGMTQKLHSRK"
     gene            19291..19704
                     /locus_tag="PL000_14055"
     CDS             19291..19704
                     /locus_tag="PL000_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90074.1"
                     /translation="MFTIDTQLSNGGAKTHLILVADDNTMVLQMLTDFLEMQGYQVFQ
                     ARSGVELLEKISETHPDLVLVDIQMPRMDGLETIRRVRASQDGQISSIPLIAVTALAM
                     PGDRERCMLAGATEYVSKPLKLNELSTLIRNQLQK"
BASE COUNT         5175 a         5416 c         4557 g         4459 t
ORIGIN      
        1 ctgcacagcc gtttctttct tctttcctct ttcctccttt ataataccct ccctatgacc
       61 tccctcacca tcatcggcgg cggactggct ggcagcgaag ctgcgtggca ggtagcccag
      121 cgtggattca aagtccgctt gtacgaaatg cgcccgataa accccacagg cgcacacctc
      181 acaggcgacc ttgccgagtt ggtctgctcc aactcgctgg gctccaacct acccgaccgc
      241 gcctcgggcg tcctcaaaaa cgagctcagg ctgttaaatt ccatgctgct tgaatgcgcc
      301 gaagcatcgg ccctccctgc aggcgcggcc ctcgctgtag accgggaagc ctttgcccgc
      361 ctcgtcactg aaaagatcca aagccacccc aacatcgaag tcatccgcgc agagatgaaa
      421 gagattcccg attctcccgt catcatcgcc agcggaccac tcacctcaga aagcctttcg
      481 cggtccattg ccaaattaag cggcgacgaa cacctctttt tcttcgatgc cattgccccc
      541 atcgtcctcg ccgaatccat caacatggaa attgccttcc gtgcctcgcg ctacgggcaa
      601 ggcgatcagg acgaaggcga ctacatcaac tgccctttca ccaaagacga atactacgcc
      661 tttgtcaacg ccttgcaaac cgccgaacgg attcaactcc actcgtttga agatgctatt
      721 cgcacaggcg tcaaagcagg tcagttcttt gaaggttgcc tgcccattga gatcatcgcc
      781 gagcgcggaa ccgactcact ggcctatggc cccatgcgcc ctgtcggttt gcgcgatccg
      841 cgcacaggca aacgccccta tgccgtcgtc caacttcggc aggataacct tgcaggcagt
      901 ttgtataacc tcgttggctt tcaaaccaat ctcaaatttc ccgaacaaag gcgcgttcta
      961 cgcatgatcc ctgggttgga acaagccgag ttcgaacgct acggccaaat gcaccgcaac
     1021 accttcatcg cctcaccaaa acttttgcgc cccacccttc aacacatcac ccgtgatgac
     1081 cttttctttg caggtcagat cacaggcgtg gaaggttaca tgggcaacat cgccacaggg
     1141 ttgctggcgg gcctcaacgc cgcacaattg cttcaaggca agccccccgt caccctgccg
     1201 cccgaaacca tgcttggcgc cctctgtcac tacatcacac atgccgacct caaagacttc
     1261 caacccatga aagccaattt cggcatcctc ccaccgctcg aactcgaaaa gcgcatcggc
     1321 aaacgcgaac gcggtcaacg ctacgccgac cgtgccgcca acgcactnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnntac ctatcttcga ttttcgctct tctggccttg
     1501 gttgcaggct ggtacatcgt ctcttttatt aaccgtgaac ctgatcctgt ttccttcgac
     1561 agttcccgcg cctatgcaga cgtgcaaacg caggttgatt tcggtccgcg cattcctggc
     1621 acagacggtc acgccaaaat ccgcgcatgg atgcaagccg aactcgaatc cgcgggttgg
     1681 accgtcgaaa tccacgaaac cgaacgcatg ggacaccctg tttacaatct catcgccaaa
     1741 cgcggcgaag cagacccaca gatcattctc ggcgcacact acgatacccg catgatcgcc
     1801 gatcacgacc cgcagcccac caacctcaac aagcccgttc ccggtgccaa cgatggagcc
     1861 tcaggagtgg cggttttgct tgaactggca cgcaccctcc ccgaagacag tgtccctgtt
     1921 tggctggtct ttttcgacac cgaagacaac ggccgcatag aaggctggga ttggatttta
     1981 ggttcacgcg ccttcgccga agaaattccc gttcggcctg aagcagttgt catcgtagat
     2041 atgatcggcg atgcagattt gaacatctac ctcgagcaga attccgacat cgccatccgc
     2101 actgagatct ggagcattgc cgcccaactt ggctacggcg ataaattcat caacgaagaa
     2161 aaatactcca tgctcgatga ccacaccccc ttcctcgaag ctggcatccc cgccgttgac
     2221 atcatcgact ttgattatcc ctactggcat accacgcaag acactcccga caaagtctct
     2281 gctgaaagcc ttggcgccgt tggcaatacc ctgctgacct ggatcagtgg aaaaacctcc
     2341 aaatagcacc gcacgaaacc accaaggccg cattttcaaa tatttatatg gtcaaaagtc
     2401 taaacaaggc gtaaacttac agcaatgacg gtacaggaat ccctgcaaaa aattcgcact
     2461 ccgtgggtgg aacgcatctc ccgtgaactc gcaagcggcg agggcgttcg cgtgggtttt
     2521 attgaacagc ttgaacgttt ttacgaactc ctcgaacaca cggttgttac gggcaatctg
     2581 gcctggcttg acccagtgtt gtacgattgg gggcaatctc ccaccgaaac gaatctggaa
     2641 cagggagatt actacgtttc tttcatagtc aaccgtatga ccacgcttac cattgaagtg
     2701 gcgcgtgaaa atctagggaa agaagatgcg cttgagttgg ttgcagccgt tgtccccatc
     2761 tttacccaca gcctgagtgt ggttgttcgc tacgaaatgg aaacccgcgt tgcacatatt
     2821 tccagtgaac tgaacaagac ccaggaaaaa ctgcaacagc ttgaccataa caaatccaat
     2881 tttatttcgg tggctgccca cgaactcaag acgccactca ccctcatcga aggctacacc
     2941 accatgatgg tggacatggt cgagcaatca ggccaatcgc ccattgacag tttgttgaag
     3001 ggagtcaaca caggcattca acgcctgcgc cagatcatcg acgatatgat cgatgtctcg
     3061 ctcatcgata acaatctgct tgcactcaac ttccagccgc tttgggtcag ccacatgctg
     3121 aacttgttga aaaacgaatt aaaaaagacc gttaccgacc gaaaacaaac gttgatcgtc
     3181 aaccaattcg aaggcagtga cttgatgatc tacggcgatt cggaacggct ctatcaggcg
     3241 ctttacaatg tagcatccaa cgcgatcaaa tatactcccg accacggcac gatcaccata
     3301 gaaggcaggc tgctgcctgg cttcattgag atcacgatca aagacacggg cattggcatt
     3361 tcacctgaaa atcaatcccg tatcttcgaa aagttcggtc agcttgggcg caccgacttg
     3421 cattcgagcg gcaagaccaa attcaaggga ggcggccccg gcctcggcct ccccatcacc
     3481 cgcggaatta tcgaagcgca tggcggaacc atctgggtag aatccgaagg gtatgatgaa
     3541 gaaaaattga aaggctcaac ctttcacatc ctattgccta cacgcacaga agcgacggat
     3601 ccactaatta ccaagttttt cggcgcaccc gggcagttaa aatcagaatc ggaaaaagaa
     3661 accattggca aagaaactcc cccaaccaac aaccccacct cgtgaacgcg cctttttagt
     3721 cggcgttgag attcgcgggg ataaatccat gctcccgtta aacgactcac ttgccgaact
     3781 tgctctgctc gcagacactg caggcgtaga cgtggtcggt gaactcaccc aaaggctgga
     3841 ccgccctcac gttgtcacct acatcggccc cggcaaagtg gaagaattaa aagcacttgc
     3901 tgaagaaaca ctcaccgatg tcatcatttt cgatgatgaa ctctctcccc gtcaccaacg
     3961 cgaattggaa aaggccctct ccattcgggt gcttgaccga accgccctta tcctcgatat
     4021 ctttgctcaa cacgcccaca ccagtgaagg catgttgcaa gtggaattgg cacaatacga
     4081 atacaacctg ccacgcctca cccgtgcatg gacccatctc gaaagacagg caggcggcgg
     4141 cggcggacgg gcaggttcaa caggcggggt tggtctacgc ggtcccggtg aaacccagct
     4201 cgaagtggac aaacgcgcca ttcgcaaacg catcacccac atcaaacgcg aactcgaaaa
     4261 ggtggaagca caccgcatgc ggtatcgtgc ccagcgcaaa cgttcacgca tcccaaccgt
     4321 ggcgctggtt ggttacacca atgctggaaa atccaccctg ctcaatcgca ttgccaagtc
     4381 tgaggtctac gtagcagacc agctattcgc cacacttgac ccaaccaccc gccgtgtaca
     4441 gctgcccggc gacgatcaag cgttgatgac cgacacggtt ggattcattc aaaaacttcc
     4501 cacttcgtta attgccgcat tccatgccac cctggaggaa atcgcggaag ccgatctact
     4561 tttacatgtc gttgatattt ctcaccccaa tgcgctcaac caatatcagg cagtgcagga
     4621 aaccttgaac gagattgacg caggtcatat tccgatgatc acagccctga acaaagccga
     4681 tttgctcaac cacccggaga acgcgatcga ggtcctgaaa gcctttccca aagcagtctc
     4741 catttctgcg cgcgaaggga caggcatcaa agatttgctg catttgatcc gtgaggaact
     4801 gtacgaatcc tacagcccga tcaatgtgcg ccttccctac caacagggcg cgctcatttc
     4861 gttgtttcat gaattgggtc aggtggaacg cgtggaacat gaacgcggcg gcgtggtcat
     4921 gcagggacga atccccggac ggctgcttgc tcaatacgcc tcatggcata tcaaaccaaa
     4981 ccagcaggaa atgacagaaa acgaagccga tgatctggag gaggaataat gtccaagttt
     5041 ttagatcgca tgtctgaatt tctggcgcat cgaaaaggat tgctccccat tattggcctt
     5101 gttctaattc tggtcaattt actcgttcaa tttattttcc caggcgggtt tttagcctcc
     5161 accaatcttt tcctgcacat tggattgctg gttgccattt tcggcttgat gctggcttgg
     5221 gcgttgtaat tttttacggc atggcccagc gtaacactgg gatcttctga acaacagcca
     5281 cgatcagata ggaaactgca aaaccaaata aacccaacag gggaatcata tataccgaag
     5341 aacccgccga aaaccagtaa tggatggcgg gtattttttc aagctcggtc aaaacgatca
     5401 catggaccag ataaattcca aaactggcac gcccaagtgg agccagaaat gcataccacc
     5461 ggtcatcaat tggaatttca cgcagcagga taaatgccgc aacagtcaac aaaacaatgt
     5521 tcacgctcaa ataactgaca aagtactggg tattccactt aagcaaattc gactgaagat
     5581 ccaaataagc agttagaaca gaaagaaaaa ggaacaggac agccaatccg tacaactgtt
     5641 tgcgggaaaa ttcgaaaagg ccaagtacat ggccgagcaa aaaatacccc gtatatccag
     5701 caacaaaata caactcaaac ccgatcttga tcggcgtaaa ttcgtgcaaa agggaaaaaa
     5761 tgggcacaac caaaaaccac agtccgcaga aataaaataa atctgttttg gatgcccgcg
     5821 ccacaaacac ccgcaagatc ggcgtaaata aatacaggcc gatcaacgca taaaaaaacc
     5881 aaagatggtt ctcacgcgga ccgcggacaa tcttcaccac atagctcact acaaggtcca
     5941 gcgaaaagcc cgtttcaaag ccttcacgct tccaaagcag ataaattacc gaccatacaa
     6001 gaaaaggcac agccaccttc accgcacgtt ttctaaaaaa caccccatag ggttcctctt
     6061 ttttcaaaag taaaaacccg ctcaccataa agaacaaagg cactgccaca cgcgagatca
     6121 caaaataata ggtcgaagcc agcacagggc ctgctcccgt ataagcaaca tgagccatca
     6181 ccacaaggaa agcaccaatt acacggatca aatccaccca tcgaagcgtc acactggaag
     6241 atgtagtatt catcctgatt cctaaaaaac gaattgcctg ctgaaaaata ttataccttg
     6301 tccacaacag ggcaatttga attaaaatcc gcacatgcca tttctgtcgc caggccagac
     6361 tctcctcaat caatatcaaa tcgaaacgtt cgtcacactc acaccgcttg gggagcttta
     6421 ccgcgcaacc gacacgcgca gcaacaaacc gctggccctt acccttctgc caaaaacgat
     6481 ttcggaaaat atcgaaaagc tgaaacacct tgaagcagaa gccgccagac tgcgcagcat
     6541 ctcacatcca aacatcgtcc cttttcttgg cttataccaa accccaacca caaacttcct
     6601 tctggaagaa tgggtaaatg gcccgtcgct tgtcgaaata cttgcaaggc ctcccctctc
     6661 cgcaactgaa gccttggtgt acacaaaggc aatctgtgcc gcactcgaag ccctgcacaa
     6721 acagaactac ctgcacttaa acctcattcc tgaattcatt cacatcaaca aacgcggtga
     6781 aattttggtc agcggaatca gcgccgccca acagatcaac gccatcatcg aatatccgca
     6841 tgggaaatat ctgcgtctgt acgcttcacc agaacagttc accgatcaaa agctcacccc
     6901 agcatcagat atttactccc tggccgccat actttacaca ctcatcacag gcgcatggat
     6961 caacggtaaa tccgcgccaa aaaccaatgc tgaaattaaa aaagctcatc ttgatctcac
     7021 gccgccagca cccatcaccc tcaacaagga gattcccgat catttctcgc ggatgatcct
     7081 atgggccctg cgaaaaaatc ccgaaaatcg actcaagacc accaccgaat tactctcatc
     7141 gcttgcgttg gcaacccaat cttcagtgga tcaagtgccg cttcgcaccg acccgaaaac
     7201 tgctcctgtc acctcggcag ttcttgaggg atggaatttc ctgccgccgc ccaagcctaa
     7261 catcatcaca gaagattcac tgccgctcga agagcgcatg actgcaattg cccagcctaa
     7321 aaagcgcaga tcaggcatgg gcatcatgac cgcattcgtt gttgtgctgc ttttgggatt
     7381 cgcttcattg ttttggttgg tacgtcccgc tcccgtcgtg attccaaccc cgatccagtt
     7441 cacgcccttt gccgcagatt ttactccgcc gcccacattc acccccgagc cacgccccac
     7501 tgacccacac ggaggtcgca ttgccttcac ctgtacacga ggtgactaca accaggtatg
     7561 cattgtcaac cgtgacggct ctggttacac tcaactttca gatatggatg ccagcagtta
     7621 ctaccccgcc ttcactcctg atggaagctc gctgttgttc gcctccaatc gcaacggggc
     7681 gtttgatttg tatcttttac agttcgggca aaagcaactg acgcaattaa cccaaaatat
     7741 tggcaatgtg gtctcacccg attactctgc cgacgggcag aaaatcatct tcgtcaacca
     7801 ggtgggcgac agcctgcccg cgatctggat ggtcaatgct gatggattga atccgcacct
     7861 cgtttatgcg ggggtcaacg aaatcgtggc agtgacgtgg tcacctgaca tgagtcagat
     7921 cgcatacgcc atgtctacgg gcgcgccatt ggaatatgag atctacgtca tgaattcgga
     7981 tggaagagaa catcgccagg tttcgcgcgg attgcaaggc attggcggca gcctcgattg
     8041 gtctcctgat gggaagagtc tgcttattta tgcaggccca ttcggtgaca aagacatctt
     8101 ctcaatcgac atcgccagtg gaaattacac ccaactcacc gatggcggca acaatgcagg
     8161 cgcatcctat tctccagacg ggttgtacat cgtcttcaac tcactgcgca acgacgagca
     8221 ggctgatctc tacatcatgc gtgtggacgg aagcaatcag attcaactca ccaatcaccc
     8281 tgagcctgat tgggggccgc gttgggtgga ataaattcaa aaaaaagagt gtgtattttg
     8341 accagaatca gttttcagcg gcgcgaagcg tgaagcggta attcatccaa ttcgggtaaa
     8401 tatttattac gcatgcacat cattagcggg gaaatttaaa taacgtgcaa gagggtttga
     8461 aataaaaaat catcacgaaa tgcaagggat ttggtattac aaaaaccttt acatgcagaa
     8521 aatttattgt atgattgctg tatcaatggc gtttttcccg gctcgaccac cggggagggc
     8581 gctgttaatc tttaacccaa aaaaggagtt tttatgaatg tccaagcaac caagttccta
     8641 tctgtcttct aagttggaag gccgcccgaa ggccgatggc agtggaaacg gcatttatgc
     8701 cctggagccc atcaacaaag gtgagctcgt agccgttttc gggggcgtcg tttatgaatg
     8761 ggacgccttt atccaccttc ctgaccgtga gcgcagcctt tgcatccagg ttgaagatcg
     8821 tcatttcctg gtgccgcgcc ccattggaga aggagattat gtcaatcact cgtgcaatcc
     8881 gaatgcaggt ctttcgggcc agattggact cgttgcaatg cgagacatca aaatcggcga
     8941 ggaagtttgt tttgattatg ccatgagcga caccatgccc tacgatgagt tcgaatgtgg
     9001 gtgcggagcc gccacatgcc gcggaaaagt cggcggcaat gactggcaaa agcctgagtt
     9061 acaaaaacgc tatgcagggt tctttgcgcc gcacgttcag cgcaagatcg atgctcagcg
     9121 caaagagaag cgggcttttg aacaggccat ccgcatggca aaatcaccga agtttccttc
     9181 tgtgacaact ccggtttacg aataaagcaa aaaactcctg tgaaattcac gggagttttt
     9241 ttatccatta tacaagggac gggagcgcaa gccattcccc gacatgtttt ggatgggttg
     9301 attttgcaag caagccctga tcagtcattc tactcacgat acgattcgtt atctcggtgg
     9361 accagccaaa caatttcaca acatccctca tctgtgccgc gccgacaagg ttgaaataga
     9421 gttccagcag tttcatccgc gcttgcgatt ctgtgatctg gcgtgcctgt tcgggcaggt
     9481 cagggaagtg ccgtggaacg atctcgtaga tgtgggaata tttccatgcg ccggcctgcg
     9541 ccacacccac tggcaggatc ttgaaatctc tttgcagttg ctccagggct ttgttgaatt
     9601 cagaatcctt ggcattggat agcttcgcca ttttgcgcag atcaagggtg ttcagggcgc
     9661 cattttcaag cagggtttca taaacttgtt ttgaagcctg tgtcagccgc ccctcttgat
     9721 aggcaatgag ataatcttca tcaggcgaac cataattttc agagagggcg taaaaataag
     9781 gggcggtctc aagtgagatc attgtggctt tgccgcgtaa tatcttggcg taataccaaa
     9841 ccttcttatc aagggcgtta tctttccaac cccaggtgat gtgacctggg tcatcgtgct
     9901 tttcggcaac gggacggtcg cccgcaaccg ctgcccacag ggagggcagg tcaatgcctt
     9961 tgatgggcca gaagtaaacg aagccgcgtt ttttgatgaa ggtcaatgcc tgggctggcg
    10021 ttgaaagcct cttgttggga ggcaggttga aggtccgcgt gcgatgcgtg tttaatttct
    10081 tcaaattgat ctgggacata tgggtcaatt gtaatatacg tttgaaacct gatgcggctg
    10141 gagcgaatac actataattt ccaaaaagga gaattccatg cccacacttc aaactgttta
    10201 catcatggct tttacccccg aaaaaatgga caacaatacc ttgatcagcg tgtgccgcat
    10261 ggcagcggat atgctctcag caaaagacag tacattcgcc cagcggatgg gaatgatcgc
    10321 caaaaccacg cgcatgacct tctcaaatca actttatgta tccaccatgc acaccccgca
    10381 aaccatgcaa accacactgg gcggctgggt aaaaaacgag aatatgccct ttgcgcccgt
    10441 gctcggtgac accttcttcc cgcatggtat gagagacccg aaaggcaggg agaacttctt
    10501 tctcttttac ttccacatca ccaattgaca aacccagccc gtagcagggc tagaaacaat
    10561 ccctgagaac tgttttttag aggcagccat ccaatctttc aagagttgtg ataggctgtc
    10621 ttttcattag cttttttgga gtatttttgc aaccatttct gccacggcgc gtaaataccc
    10681 ttgaaaatcc atcccattcg ggataagtaa atttacaaag gaaagcacca tgaaacacac
    10741 aactctgatc gctcttttgg ttatctccct ttttgcaaca gcctgtggag ctgcccccgc
    10801 cactgcggaa cccaccccca ttccaaccgt ccttgccgat aacaccatca ttgccgaggg
    10861 caaccttgaa ccgatccact acaccgaatt ggccctgaac gccagcggat tggttagcga
    10921 ggtcttgttc agcgaaggcg atcaggtcgc cgctggcgat gtaattgccc gcctgaaatc
    10981 gaacgaagcc caaaccctcg aaaccgcaca agccaaggca ctggccgaaa tgaccgccgc
    11041 ctaccaggaa gtgcgcgatg cccagttcaa gctggataat tttgacgtcc cctccgattt
    11101 cgatgggatg accccatctg aagcggtcag cgaaatgctg gtcaaacttg ataaagcccg
    11161 cgctgatttc gaaccgtaca aaaacctcag cgaacgcagc ctggcccaaa ccgaagccga
    11221 aaagaacagc ggtgtggtca ctggtacagc gaaaatctac aagcaagcgc ttgatgatgc
    11281 ctgggcacgt taccgcaagg ccattctctg gcttgaactc gaatcgaccc tgcaaaacgc
    11341 aaactcccgc ctgacccttg cccaaaaaga ttacgaagcc ctgcaagacc catctttttc
    11401 agcggatacg gctggagccc gcgccgccct cgccagcgcc gaagtacgcg cccccttccc
    11461 tggcctgatc acagacttga atctcaaagt cggagaattc gccgcttcgg gtcagcctgt
    11521 catcaccatt gccgacaatt caaaatgggt cgtcaaaacc accgacctga ctgaaattga
    11581 cgtggtcaag atcaaggaag gccaatcggt cacagtgacg ttggatgcgc tccccgaagt
    11641 ggaactgagc ggcaccgttc tctacatcag ccaggatttt tccgaaaatc aaggtgacgt
    11701 tgtttacgaa gtcaccatcc tgcttaccga acaagaccct gccatgcgtt ggggcatgac
    11761 cgccatcgtc aagttcgtgg aataaaaaaa ccgcttcgcg ctggaccttg aaactgcttc
    11821 tgaatcaaaa aggagtgaca tcctttgatg tgatgcactc ccctcttcaa acatcggcgc
    11881 aagaaaggca cgtatataat ggcattcttt caaaaaaaca accacaccag cacagacacc
    11941 cgccccatga tcgacctgcg cgatgtgaac aaattttata aaacagccat cggcgattat
    12001 caggccctca ataacataga cctgcagatc aacgcgggtg agtttgtcag cattatcggt
    12061 aaatcaggca gcggcaaaac caccctgtta aacatgatca caggcattga catccctacc
    12121 aatggcgaag tctgggtaaa tgacacggcg gtacacaaac tcagcgagaa caaaatggcc
    12181 cgctggcgcg gcaagaacct cggcgttgta tttcagttct tccagctgct gccgatgatc
    12241 tcagtcatcg aaaatatcat gctgccgatg gatttctgcc gcacctatcc catgcgcgaa
    12301 cgtcatcagc gggctatgca attgcttgaa ctggtggaac tcgcagacca tgcgcacaaa
    12361 ctccccaccg ccctttcggg tggacagcag caacgcgtgg cgattgcccg tgccctcgcc
    12421 aacgacccgc ccgtcatcat cgctgatgaa cccacaggca acctcgactc aaagacagcc
    12481 gaatctgttt tctcactgtt caacgacctc gtctccaagg gcaaaaccat catcatcgtt
    12541 acacacgata gcgccctcgc aaaacgtacc caccgcacag ccctcatcgc tgacggtgaa
    12601 attgtcaacg agtatgtagc caaagccatg cccacactga ccgcggcgca attactgcaa
    12661 ctcacacgca gtgccaaaac cctgcaattc gagccaggcg ccatgatcct ctcagaaggg
    12721 actcatgccg atactttcta cgtcgtctcg aaagggacag tggaagttgt cctgccgcgc
    12781 accaatcaat cggatgtggt ggcgcttcaa cttggacctg ggaaatgctt cggcgaaatt
    12841 gaattcttcc acgaaaagaa acatctggct tctgtccgtg cctctgaaag cggacccgtt
    12901 gaagtggtct ccatcgagta tggtcaactc aaagaattgc tggaccagtc tcaggtcacg
    12961 cgcgatgcgc tgcaccagat ggcagacaag cacgaagacg agcatatgtc cattcgagga
    13021 ggctccaaat gagttcgcgt tggaaaaagg tctgggctga tttttggggc aaccgcggac
    13081 ggaccttcct caccatcatg accatcatgg tgggcacctt tgcagttggt ttcaacagca
    13141 acatgggtgc ttatatgatg caaagcatgg acgatgatta tctttctgca aaccctgctg
    13201 aagcgcaggt ttatgcaggc ccgcttgacg acgacatggt gaagatcgcc cgaaccgtcc
    13261 ctggtgtgga gcaggtgcag ggattttcat cggtcggtgc gcgcattgtc cgccctgatg
    13321 gaacctacac caacgtcaat ttcatcgcac tggaaaaccc tgggaaactc acgctcaacc
    13381 tccttaaacc agtcacgggc gagatcagca ttcccacata cggaaagaac gagaccatct
    13441 tcgatggcag tgcaatggga atgggttaca aagctggcga caccattttg gtggaactag
    13501 ccgacggcaa gagacgcgaa ttgaagttga ctggttacat gcatgatgtc actggcattc
    13561 cattcaacct tgccaacaca gtcggcgcat acgtaacacc cgacacgctg gaatggctgg
    13621 gcggcgacac ctcaagctat gaaatgctcg ccatcagcgt ccttgaaaag caaaccgatc
    13681 aggagcatgt aaccacagtg gcgcaagccg ttgcagaccg tgtggagcgt gcaggcgcaa
    13741 ccgtgttttt cgtgaatgtg tatgagcctg ggcaccactt tgcatggagc atcacccagg
    13801 gtgtattctt cgtgttgagc atcctcggct acctgaccgt tttactgagc ggcttcctca
    13861 ttatcaacac cattacagcg ttgatgaccc agcaaacccg ccagatcggt attatgaagg
    13921 ctgtaggcgg tggaaccatt cagatcacag gcatgtatgt tgtgctgatt ttagggtttg
    13981 ggttgggtgc gctggcaatc gccattccat tggccaacct tgccgcagag gaaatcggcg
    14041 gcggcatggc acaatggctg aatttttatg cctcgccata caaagggtat acatccacac
    14101 tgattcaaca agtgatcgtg gcactggtgg tgcccttgct ggcagccatc ctcccaattt
    14161 acaacagtgt acgtgtgacg gtccgtgaag cgatcagtga ctatggcctc ggtggaaacg
    14221 cccgccccaa ggcagactct gtcaaccaaa agtctgtgct cattccgcgc ccaattcgca
    14281 tttcgatccg caatacgttc aggcgcaagg tacgtctggg aatcacgtta ttcacccttg
    14341 tgttggcagg agcagtgttc attgcggtgt acaacctatg ggcctctttt gataaggtga
    14401 tgcaggatat tcagggttac ttcctagcgg acatcaatat tgccttcgac cgcggctacc
    14461 gttttgataa agtagccagc atcacggaaa acatcccggg cgtggaaggc gcagaaggct
    14521 ggctggaata cccaggcacc ctcaaaatgg gcgatgacga atcgggaacg caaatccttt
    14581 ttgtggcccc accttccaca tccactctta tcagcccgat catttccaca gggcgctggc
    14641 tgcagcctgg cgatgaaaat gccgttgtca ttggcaatca cctgctcaag gtcttcccag
    14701 acctgaagat cggcgattgg ctgaccatag aaaccaatgg caaggaaacc gattggcaga
    14761 tcgtcggcac gtatagcctg cctggcaatg tcagcccgcc gttgttgtac gccaattacg
    14821 aatacctgag ccagctcgtt ggcgaaattg acatggccta ctcactgcgc atcaccacca
    14881 cccaacacga ctccgtcacc caaaagcgga tcaatgatat ggtgcaggcc gcctttgaag
    14941 cgcaaggtgt tcagatcaga tccacacagc ttagcgcaga ttttatccgc gaccaaaagg
    15001 cacaaaccga tatcctcgtc tatttcatgt tggtcatggc aaccttgatc gcaatcgtcg
    15061 gcgggctggg tttgatggga accatgagca tcaacgtatt ggaacgcacc cgcgaaatcg
    15121 gcgtaatgcg ggccatcggc gccagcaatg gcgacattca agccatcgta attgttgaag
    15181 gcatggtgat cgggatcgtc agttggctga tcagcattgt gatcgccatc ccgatcacga
    15241 acatcctttg ttacggagtg ggaatggcga tccttaccgc cccgatgcct gctgtctacg
    15301 gcgtgagtgg aatcgccgcc tggctgatct tcacgatcgt gcttggatca ctcgccagcg
    15361 ccctccccgc ccgccgcgca tcacggctga ccgttcgcga tactctggca tacgaataaa
    15421 ctgcttttga aaaccaaacg cctcgcatgg aggaattcca tgcgaggcgt ttctcttttt
    15481 tacaacaaac ccagacggga ttcaactctg cttacacttg aggggcatgg gcatctttga
    15541 atgagacggt aaaggtggtt ccattttgtg gtgcgctttc cacgctgata tcgccgccaa
    15601 gtttttcgac aatggatttt acgatgtaca agccgatacc actgcctggg atctcagcga
    15661 tcgtcacatt tctggctcga taaaaacgct ggaaaagatg cggcatggca tctggaggga
    15721 ttccgatgcc ctgatcggcg atcgaaatat gtacgtaccc attccccttc cacaagccaa
    15781 ccagaacagt gctgcccttc ggcgaaaact tgatggcatt attgatgagg ttgatgaata
    15841 cttgctgcaa tccgctttta tcgcctgcga tgtaaaaatc actttgttcg ctcatcaact
    15901 taatggaaac atcccgctta ttaccaatgg gcctgaccgc catgatcgat tcttccaata
    15961 ctggtgtcaa atccaaaagc gtagactcca actccatcat accgctctcc aggcggccag
    16021 cgatcaacag ccgatctatc aatacttttt gacggttcaa ttcactggtt agcgtccgcc
    16081 agtattgatc taattcttcg cggctgccac catcttgaat taattcggtc attaggaggg
    16141 ctgtcgtcaa tggggtgcgc aattcatgcg aagcgcggtt gataaaatcg gacttcatgc
    16201 ggtcgagacg ggcttgatcg gtgacatcac gaatgacaac caatacttct gtttcactga
    16261 tcggcaaaag gcgcgattca taatcacgag cgctaaaggg cagggtaaaa ccaaacaccg
    16321 ggcgttgctc ttcatcatct tcatccacat ttgggacggt cagaatgctt tgcacggttt
    16381 cttcgggcca gatatcgctc aaacgcctgc ctgagatcat gtcacgatgc aggtagagcg
    16441 gatgatcggg gttcgtagtg taatcaagga tgaccccatc ggtggtcagg cggatcaaca
    16501 gatcaggcag aaccgaaagg atgatgcggt tccgattttc attttctcga atcgtctctt
    16561 cataactggt gcgctgcaag gccgagccca tcaaccctgc ggccatttca accgcatcac
    16621 actcggaaga agaccaggca agcaatttgg aagtatcaaa aacgccaagg aatttccacg
    16681 aatcgagtgt tccataaatg gggaaaacaa aaaaagatgg gccgtgtcta ccgacgtcat
    16741 tattgaaaaa ttgtccaccc gaagccttca aaatggaggg aatgattggc agaatgagat
    16801 ccgaaacatt caagtctggg gtgtcattct tgatccattg atactgtgtg aggaccgcag
    16861 gctgcggcga aaaagtgttg acctcaaaaa tagcgcaacc cgttacaccg atcgcttcgc
    16921 caagcgctgc cagggcatca gggatcattt cgccgatctt tgaggaacgc agcaactggg
    16981 cggtggcatg attgatggcg gaaagaatgc gcgaatatct ggtcagcgcc agctcagatt
    17041 tcttcctctc ggaaatatcc cgcgcaatgc tttgaatgta ggcaggctca tttcgttcgt
    17101 tgtagatgat ggaggcactc acctcgacag gaaaagtgga gccatccttt cgcttgagga
    17161 tgcgctccac catatttgtg tcatcgtcca acacagtgcc atcttccacc ccatcactca
    17221 acgacatgac cgaactgacc ggtaagccga cgatctcttc ttttttgtat ccaagcaggt
    17281 caagcgcctg gcgattggca gtgatgtaat gcaggtccat gccaatgata aagacaacct
    17341 cacccgtctg atcgaacaag gcacgatagt gaatgtcttc gccccaatcc actacttttt
    17401 tctgcttgtt cagcaaggcc atcaaatgcg gaggtagatt ccctatcggg acggtttttt
    17461 catccatagg tcacgtcacc aggttgaggg tcgggaagat gcgcctgtat atgacatcca
    17521 cgcaatgatt caagtatttt cacttcgcca cgcaggcttc taaatacagc acgtgcaatc
    17581 gaaagtccca cacccatccc ttcaaattca cgggcatcac cctggctgat ctgataaaaa
    17641 cgctcgaaca ccttttcatg caaacgtgat gggatcccgg gaccattatc aaaaacatcc
    17701 agcgttgcgc cgccgttccc agtgggggtt atgcgtagat gcacctttcc atttttagat
    17761 gtgaacttga aggcattatc caccagatga acaagggcat gcgtaaactc gcgacggggc
    17821 gctgaaattt ttccgcttga agaaatatcc attgtaaaat ccaggccttt ttcgtcgtaa
    17881 cgatcgaggc gccttttaac aatggaagcg atatccattt ccatatcaat gggttggcgg
    17941 atggtattta aatttccctg atcgatatta cttaataaga tcaaatccgt agtgagcgat
    18001 tcaagtcggc tggcgcttga gagggccttg cttaagaacc gactctgctc ctgggcatca
    18061 tcgaatttgt tattgatcgc caattgcagc ggcatcatga tgttcgaaag cggcgtgcgg
    18121 atctcatgat ggaaattctg caaaatctcc tgacgcactc tttcaagatc tgcttcggcc
    18181 gctacacggg cctgttccct accattctct tcagcgatct ttacacggcg cagcacggcg
    18241 ctaattctgg cgatcaactc gtacaccaca aatggtttcg taatatagtc atcagcgcca
    18301 tccataatag cactgacttt atcctccaca ccggagcgtg cggtaagaaa aataaaggga
    18361 atggaagcca attgaggatc ctggctcatt atcctacgaa gctcaagccc atccggagga
    18421 ggcatcatga catcagaaag gatcaggtcg ggtttgatct cttttgcttt tgcaaggccg
    18481 tcattcccat cttctgccga aacaacctga aaaccatatt ttttgatggt ggcagataca
    18541 cccatcaaca aggcggggtc atcatcaatg atcaagataa gtggtttgga attcatgttc
    18601 attttctcga atgcagtttt tgtgtcattc ccatggcttt accagccagt ttggcgttat
    18661 tgttcacgaa catgacctgt atgcaggaaa acgaaatcaa atcactggag atcaacacgc
    18721 aacgatcaat acgtttgcta tttacgtagg tacctgccgt ggaattatta tcgtacagaa
    18781 tgaacttccc atcttcatag cgtatctcag catggaagcg cgagacggac tcttcctgca
    18841 aaacgacatc atttcccaat tgacgcccaa ggcgggtcac ctttttctca agcggaatca
    18901 tttgacgatt gatgataaga aaaacattct gttcccgtga agggataagg ttgttatctt
    18961 cttcctgtgt catggggaga tcctccgtaa aaaataaatc ataattcaga taatacaact
    19021 ctcttataca gtgagtatac atacgaagcc gtgaaagccc actcagttta gtacttaatt
    19081 cgaatacgta aaactacttg atatgcgatc tacagcaaaa tcaccctcaa tgactcatgc
    19141 aaacaaatgc acaccaaaaa ttaagtaaaa atacggatgg cggccttgtg acagtgccga
    19201 taagatgtat ttggtgaaaa acgattgtaa taagcaactg caatcgactc gtgttcctga
    19261 ttaaccgtac aactccaagg agttttcaat atgttcacga ttgataccca attatcgaac
    19321 ggcggcgcta aaacacactt gatcctcgtt gctgacgata acacgatggt tttgcaaatg
    19381 ttgaccgatt ttctggaaat gcagggatat caggtatttc aagcccgcag cggagttgaa
    19441 ttacttgaaa agatctcaga gacccatcca gatcttgtgc tggtggatat tcaaatgccg
    19501 cgcatggatg ggctggaaac cattcgccgt gtccgtgcaa gtcaagacgg gcaaatctcg
    19561 tccatcccgt tgatcgccgt tacagccctg gccatgcccg gagaccgtga gcgctgcatg
    19621 ctggcaggag ccaccgaata tgtgagcaag cctttaaagt taaacgagct ttccacctta
    19681 attcgaaatc aactgcaaaa atagagg
//
LOCUS       DAOQLC010000081        19432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_982_length_19432_cov_22.903752, whole genome shotgun sequence.
ACCESSION   DAOQLC010000081 DAOQLC010000000
VERSION     DAOQLC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 19432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_19432_cov_22.903752"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /gene="aroC"
                     /locus_tag="PL000_14060"
     CDS             <1..1080
                     /gene="aroC"
                     /locus_tag="PL000_14060"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC90075.1"
                     /translation="IINKELARRQQGYGSGGRMKIEKDTVQILGGVMGGETTGAPLAL
                     LVQNDDHVKWKGKAIEPMTAPRPGHADLTGAVKYGYKDLRPALERASARETTMRVAAG
                     AVCKHFLSQFGIIVGGYVASIGEVTADFGDMPYEERFTRAEASDVRCPIDSSAEKMRA
                     EIEKTIHGKNTLGGVLEIVALNLPVGLGSFVQWDRRLEAKLAMAIMSVQAIKGVEVGD
                     AFENAKRIGTEAHDPINLQPSTFDLQRSTNRAGGTEGGVSNGQPIIIRAAMKPIATTL
                     VPQQTVDLASGENAPTKYERSDFCPVPRAVPILESMVAFVLADALLEKIGGDSMNEMK
                     SRFETLRKATLEDLPMDNIPHIFWE"
     gene            1176..2714
                     /gene="aroB"
                     /locus_tag="PL000_14065"
     CDS             1176..2714
                     /gene="aroB"
                     /locus_tag="PL000_14065"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNC90076.1"
                     /translation="MTHIFLYGPPGTGKSTVGRKLAQNLNLPFIDLDRVIEEEAGMSI
                     PQIMEQQGEGTFRDLETASLKKLAGEQESIVALGGGALLRDVNRAFAEQHGRIILFMA
                     ELSTLLERLHYEAGKRPLLAGDLKPKLTALLEKRREHYHSFPLMIHVDGKSAEQNAYQ
                     AQLAAGRHHLSAMGEYDAVVQSRGIDQLGEMLKTRGLQNPILVTDDNVANLHAEKAVT
                     ALRNSGFDPRVLTVPAGEAYKKLETISQIWKGFLEAGLDRKSTVIALGGGVIGDMSGF
                     AASTYMRGIAWVGVPTTLLSMVDASLGGKTGFDLPEGKNLIGAFYPPKLVLADPQLLD
                     TLPEAELISGMAEVVKHGIISDPELFSLCGRGLDWVKANLEEVVRRGMAVKIKVIEED
                     PYEKGVRAALNLGHTVGHAVELVSRFNLRHGEAIAIGTVVEARYSAQIGRAGHGLVDA
                     VTSSFKSLGLPVQIPDAMPRDEIIRVMRVDKKKTAKAIRFALPVEIGKVELVNVDDLE
                     SVLE"
     gene            2717..3151
                     /gene="aroQ"
                     /locus_tag="PL000_14070"
     CDS             2717..3151
                     /gene="aroQ"
                     /locus_tag="PL000_14070"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNC90077.1"
                     /translation="MKILILHGPNLNLLGTREPEVYGSTKLHELNESLIALGKEFNAE
                     VTCLQSNHEGALIDALHEARTQANGVVFNPGGYTHTSVALRDAISAIQIPVIEVHISN
                     VHAREEFRHKSMISAVCKGTVSGFGLKSYELGLRGLIDIIRS"
     gene            3148..3939
                     /locus_tag="PL000_14075"
     CDS             3148..3939
                     /locus_tag="PL000_14075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC90078.1"
                     /translation="MNTQIIPTTEPFFLPGGRTGVLLIHGFTGAPKEMRWMGEYLNGQ
                     GYTCLGIRLHGHATTPEDMIASRWTDWTASVEDGFHLLSGSVDRIFFAGLSMGGVLSL
                     LMSTKFADRVSGVVAMSTPYSLPNDPKDYSPAYISLYSHFVKYMPKSGEVPGASWFDK
                     EAFKDHISYPQNPIRSIAELKLLLGEMQAALPRLTMPVLLIHSKDDPYVLPENVEKIY
                     AGLVNASDKTKLLVTGSGHVVTRDAARHQVFEAARDFIRRVENQS"
     gene            3955..5082
                     /locus_tag="PL000_14080"
     CDS             3955..5082
                     /locus_tag="PL000_14080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90079.1"
                     /translation="MNRNLTFVVVSLLFWGIGEGMFLNFVPIYLGKEFALGEEQIGLA
                     LGMFGFFSAITHMPSGYLADRIGRRPLLITAWILGLASTFTMGISTALPFYLVGLFGY
                     GLTAFVSSPLSSYVTAARGEMPVGTALSLVSASFNIGMALGPVTGGWIGDHYGMRMSY
                     LVAAVIFLFSVASIFFIKNQPLDKHDPEAPPVTLWKNRGFISFLGMVGFAVFAMYLAQ
                     PLTPNFLEGVRKFSLSDTGWIFSAGALGNALFAIGFSRLDPRRGFLFAQVLVVLFALI
                     MWKGFGLPLFMLGYFLLGGFRAARPMAQAQGRELVHQSQMGLMYGTLETVNATIFIFT
                     PPLAGYLFVIDPFLIYPLAMGLITLAMISTFIFSYRKVQHA"
     gene            5075..5713
                     /locus_tag="PL000_14085"
     CDS             5075..5713
                     /locus_tag="PL000_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC90080.1"
                     /translation="MLEIVSFTLGPAQTNAYLVADPDTKEAVVIDPSWDGHIILAEAQ
                     KRGWRIGHLWYTHAHFDHIGGAAAIADALNPLPHVALHPDDHVLWRAGGGGAKFGLNI
                     DPGPEPTIDFVQGMLLKLGSNEFEVRYTPGHTKGHCVLYVASENICFCGDVIFNGSIG
                     RTDLPGGDFATLENSIHTQIYTLPDETRLLSGHGPETTVGHEKMYNPFVRTG"
     gene            5750..6889
                     /locus_tag="PL000_14090"
     CDS             5750..6889
                     /locus_tag="PL000_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC90081.1"
                     /translation="MKNPNAQNQKTNSAPSTPPSNPNPRPSFFSVIPGWVVGLVVAVI
                     FVALLASVSKSFWYFEVIENDQVGITIEAGEIQSVLQPGIAYDFGLFVDLVKITTSAV
                     AITVDDPELITIDKQRIGLEVTADVFRPREADIVISNYSRYRNIYLNDESLQQRMTAF
                     TLQAMKVCVGDKKFDEAVIGSGRDDLRACIDDELSGLAEPLGLEVRNVAVPQITISPE
                     VQAGLDAIVQSRLATEKAKQDAEKAKQEAIAQQAVEEGRIRVDQAKLQEEARQQAILQ
                     DIKRQQLEAELAVIEQEQINAQAKLELTDLLQQVADEQALVDIAKELALAALYEKYPS
                     FVTLQIALANASAIKPTDKFIFTQEGVFPNLIFSNGTLPTFNVNP"
     gene            6938..7828
                     /locus_tag="PL000_14095"
     CDS             6938..7828
                     /locus_tag="PL000_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90082.1"
                     /translation="MKRAVGISLGSSKRDKKVTVRLNQVEIQVERIGTDGDVEKARQM
                     YLDLDGKVDAFGVGGVDLYLRLDQKEYPLHAALKLVSGVKQTPLCDGRGLKHTLERRV
                     FQLARSTLGEIHFKQAFVPVAVDRLGLAEAVAEVSERTVFGDLMVALGVPIPVYGIPA
                     FKRVARVMLPMVSHFPMSMIFYGSDGAEHEPKYVKYFEESDLIAGDFLFMRKYMPKSL
                     AGKTVVTNTTTEENIALLKERGVKTVITTTPRYDGRSFGTNTTEAMLTAYAGKGRRLT
                     DDELNGLIDELGLKPNVIQF"
     gene            complement(8050..9225)
                     /locus_tag="PL000_14100"
     CDS             complement(8050..9225)
                     /locus_tag="PL000_14100"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC90083.1"
                     /translation="MKTSYLTDLTCSDCGRVFSINEVLTFCPDCQSTLISNYDLNAAR
                     ANVDRDEIHTRSKGMWRWHEMLPIQDPENIVTLGEGDTPLLRLPRVGNELGLSNFFVK
                     DESSNPTGSFKARGLCAAVSRAKELGVKKVIIPTAGNAGGAMAAYAARAGLQAMIYMP
                     KDTPNANIQESRIAGAEVILVEGLISDAAGMAGVKAREEGWFDVSTFKEPYRAEGKKI
                     MGYELAEVFDWTLPDVILYPTGGGTGLVGMWKAFAEMEALGWLKNTKRPRMVSVQAEG
                     CAPIPKAFHAGASFCDFWLGAQTVASGLRVPKSFADRIILSNIRESNGTAVAVSDEAL
                     MRAQKKLAAMEGIFAAPEGAACLAALEELVKQKWVQPDEKIVMFNTGSGLKYLDTMN"
     gene            complement(9260..9649)
                     /locus_tag="PL000_14105"
     CDS             complement(9260..9649)
                     /locus_tag="PL000_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90084.1"
                     /translation="MGNNVLYIEDNPDNMMLVRRALESRGYRLVQALTGLDGVNKAET
                     EEVDLILLDINLPDIDGYEVARRIRSSDKKHALAYVPIIAVTANALKGDAEKALSAGC
                     DVYMSKPINIRELWARVEAFVPSPNSK"
     gene            complement(9664..11307)
                     /locus_tag="PL000_14110"
     CDS             complement(9664..11307)
                     /locus_tag="PL000_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC90085.1"
                     /translation="MAKPQFLQCAICGNQQPYEPFVPAVCKSCGSQWLEAKYDYAVFK
                     RNILRGLPDRPRNLWRFQDVLPLENPSALDLYPAGGTPLWLSQRFATKLGHDRVYFKD
                     ERYGPTSSFKDRQAAVAVAAMNENNIREAVIASTGNAAVAYAAACARAGIKLWVFMTS
                     LVPQEKLREAALFGAEVIRVSGNYDQTKQIASQFAQRRNLHLDRGASSIAARESMKTI
                     SYEIVEQLGWRAPDWYIQAVSGGMGPLGVYQGFKEMLDMGLIDRIPKIAIIQAEGCSP
                     MVKAFKADKEVADPVVPDTRIIILSTGDPGKTYTYLYGILKKHGGTMESVTDAEAFDA
                     MRSLAKSEGMAVEPATAVAYAGLEKMLREKIITKDETVVVNCTGHTFPVEKHVLGDQW
                     AVDVHLSKDQSPAPQEGLQAALENLDEKATTVLLVDDNSDDALLIRRLLEGKKSYRVY
                     HAKDGWEGLAMARQHLPDLIISDLTMPGIDGFGFVEELKLDPRTKEIPVVVVSAKDIT
                     AEERQRLNGRIEALYQKGSLPTRKFVDKVINVIEDKNGA"
     gene            complement(11312..13768)
                     /locus_tag="PL000_14115"
     CDS             complement(11312..13768)
                     /locus_tag="PL000_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90086.1"
                     /translation="MHPEDKSQKRVEKLFSDLEQIAGQAVPSSGDSAAARPTSFPVRQ
                     EAPPVDPVVTALTARIHELEAKLEESNLRIAMAEAMLATEKTETARIPAIVYEKEKVG
                     YAFKDENILPLRDAQIAAKELEDAVDAPLIMGGDVIGDIKIEPPSDRPWTEEEERLAS
                     AVAQQASLQIQNLRLLSATERARTEAQAATRRFTHESWESFLDGIRNSERIGYLYDQS
                     AVEPVRDIPEHDHRETVKVLDEQIGNLFIKSDPARPLSEEDKEMVAAIARQVSQQVEN
                     LRLLADASRARADAEDATRRLSRESWQSYTQRNQSSLGYVYDSIQVSPLTDEFSNAEN
                     ISLVQPLTVRGETIGRLVTLAPQTHAGGATPNRITPEAEGLAAQIAERVSEQLERLRL
                     TEEIQKRAAELATVATVSTAASTVLNPDELLQQVVDLTKERFGLYHAQVFLADEAWNT
                     LLLAAGSGDIGKQMVMSGNTIPMEKNELLVSRATRERKALISNDVRREESFQLNPLLP
                     DTRSEMAIPMIAGDKVLGVFNVHSVNLNHFTEEDANIYTTLASQVAVSLQNARLYAEQ
                     AATVAQLRELDRLKSSFLANMSHELRTPLNSILGFSDVMLEELDGPLTENMRNDLGLI
                     QKNGQHLLHLINDVLDMAKIESGKLNLNIEKFNLQEVIEEVTSITAPFASEKNLSLFL
                     EQDSDNEVQISADKIRIRQVMINLVNNAIKFTERGKISIRAACEDNHVLISVKDTGIG
                     IPHDHLDSVFQEFTQVDTSTTRKAGGTGLGLPISRRLIEMHGGKLWAESSGINGEGSI
                     FYVFLPIEARLRTEEELKVK"
     gene            complement(13793..17368)
                     /locus_tag="PL000_14120"
     CDS             complement(13793..17368)
                     /locus_tag="PL000_14120"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90087.1"
                     /translation="MDNKKTYPLHLDDESRQHTRWGTILLSTILVLVYAYVLTQIFIG
                     EKTTQSYIAMAVLLLVSTSTVMGLVLSIRQRTELGLKLTYYSLIALCVAAVVLFQGRT
                     TSITFSILSISTVGFIWLFPQHTRREYATIMIVSLVAIWVTELLNPAWRLSMNMAQVG
                     PAGAGIFVILFAIYIFRESQNVISSNLRLKITIWTGLILTGISIILIVYSQIIARQAA
                     VDAAEKEALSFAESQARSVRADVEIPLDTARALAHALTSTKEPSNDEVLSRTQVNAML
                     RQVLLENPNFLGTYTLWEPNAFDGLDQTFKGTAAHDNTGRFIPYWVRGDDGSLTVTAL
                     LDYETPGLGDWYLIPRQTQKEVTIAPLIYPIQGVDTVMASFVVPIIYADKFYGIAGVD
                     APIAFVQNLVDQVDLYNGKAGAVLMTSDGTLISVRNQPELVNQPAAEIFPDFLELQSR
                     IQAGESFISLSPDGDSLRAFAAVDLGQTGDHWSFVLLVPFSEIIAPANTAAIRQGVIG
                     LVLISTALAILWFLSGQIVRPVRELTTVANAISQGNLNVTARVQAADETGLLASTINL
                     MITQLRESFVTLEERVAERTQNLELASQVGRSVSQLRKLDVMLKDAAEIIRSRFDLYY
                     VQVYLTNPSKTYLNLQAGTGHVGEELLARNHRLPINTGSINGRAAVEKRSVIIADTST
                     STTFRPNPLLPNTRSEMAVPLILGETVVGVLDMQSETAGALNHEMLSAFEALAGQLAI
                     AIQNANLLAEAEQARAEVEAQAQRLTRTNWASYLDAIHKPEESGYVFEQNKVMPMVEA
                     EDQTVNDDALTAPIAITGETIGNLVVELEGQSPLTRPNELINIVARQVAQQIENLRLL
                     ENAERYRVEAEEASRRLIREGWKTYKEGAKNNLSYLYDLKEVRPLNGDSTSEPALSIP
                     LKVRDELIGELAIQGMNLEDGDSASLAAAVADRLSSHIETLRQYDQAQSALEQSEKLF
                     DASRRLTQATSLQDLVAATVESLDIPVVNRAVLGIFNYGADDKLESMSIEANWWNGTG
                     HNVTAIGVRYPVAVFRTVSLFASPVPLFFADAFNDERADAATLQLAKQLNLRAVAVLP
                     LYIGTRQVGVLMLEAEQPYHFSQDEIRLFSSLAPQIATVLENRRQFDRAQQQAEREST
                     LNLISQKIQSATTVDAVLQIAARELGHALGAPMTIAQLSMKDKEK"
     gene            complement(17449..19257)
                     /locus_tag="PL000_14125"
     CDS             complement(17449..19257)
                     /locus_tag="PL000_14125"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNC90088.1"
                     /translation="MRLTRKLLTPILIFISITLIGLFTYFSITAVRQFDESEANHLED
                     IHKEFLTEIKTKEDLAIALAYEVANNPEIQAAFAARDRERLAALTLPSYQVIDAQFDI
                     PQYQFHLSPATSFLRLHSLDQYGDDLSSFRQTVLIANQEKRAVSGIEIGRGGLGIRGV
                     VPVTYQGRHIGTVEFGPNVDLTLLEALHTKSGANLQILLERNAAEIATFTGVEGKSQG
                     PIDSLILQASTLETVFFGPITNYEQALAGSATLEHFDYQGHSYAVLTAPLYDFSGKVI
                     GVVDIISDHTAVAQQQTRQVMFYIGILVVMLLTIGFGFSFLASRTLTPISDLTITSKA
                     IAEGDLTKRAKIQSNDEIGVLADAFNTMTSQLQNALQDMEKRVEMRTKALSTSAEVSQ
                     RLSSATDPQRLAVEVVEQVQAAFKYYHAHIYFLDEESGDLIMAGGTGDAGAAMLARGH
                     KIPHGRGLVGRAAATNAPVLVPDVSKAEGWLPNPLLPDTRSEVAIPISSGRQVLGVLD
                     VQQNTVNGLDQEDVRLLQSLAGQVAISLQNARSYEQSKSQAELETLVNTIGQKIQRAN
                     TVEETLQTAIRELGLALGATRVKAAISARNNSDNKK"
BASE COUNT         4416 a         4763 c         5282 g         4971 t
ORIGIN      
        1 attatcaaca aagaacttgc ccgccgtcag caaggctacg gctcaggcgg acgcatgaag
       61 atcgagaaag acaccgtgca gatcctcggc ggcgtgatgg gcggagagac cacaggcgca
      121 ccgcttgcat tgcttgttca aaacgatgac catgtcaaat ggaagggcaa agccattgaa
      181 ccgatgactg ctccacgccc aggtcatgcg gatttaacgg gtgcggttaa atacggctac
      241 aaagacctgc gccccgcgct cgaacgtgcg tctgccagag aaacgaccat gcgtgtcgcg
      301 gctggagccg tgtgtaaaca tttcctctcg cagttcggaa tcattgtggg cggatatgtt
      361 gcctccatcg gtgaagtgac tgcagacttt ggtgacatgc cctacgaaga acgcttcacc
      421 cgcgccgaag catcggatgt gcgctgccca atcgactcct ccgctgaaaa aatgcgcgcc
      481 gagatcgaaa agactatcca cggcaagaac acacttggcg gtgtccttga gattgttgca
      541 ttgaacttac cagttggttt aggaagtttc gtgcaatggg atcgacgcct tgaagccaaa
      601 cttgccatgg cgatcatgtc cgtgcaagcc atcaaaggcg tggaagtggg cgatgcattt
      661 gaaaatgcaa aacgcattgg cactgaagcc cacgacccaa ttaaccttca accttcaacc
      721 tttgaccttc aacgttcaac caacagagca ggcggcaccg aaggcggagt ttccaacgga
      781 cagcccatca tcatccgcgc ggcgatgaaa ccgattgcca ctacccttgt cccacaacaa
      841 accgttgacc ttgcctctgg cgaaaatgct cccaccaaat atgaacgttc cgatttttgc
      901 cccgttccgc gtgcggtgcc aattttggaa tcgatggtcg ctttcgtttt agctgatgcg
      961 ctcctcgaaa agatcggcgg tgactcaatg aatgaaatga aatcgcgctt tgaaaccctg
     1021 cgcaaggcaa cgctggaaga tttgccgatg gataacattc cgcatatttt ttgggaataa
     1081 tttgacgacc gaccaccgac tatcgcccac attcagccct ccactcacta ccttcaactt
     1141 tcaaccttca acctgaaacc tgaaacttca accccatgac acacatcttc ctttatggcc
     1201 ctcctggtac tggcaaatcc acggttggga gaaaacttgc tcaaaacctg aacctgccgt
     1261 tcattgatct ggatcgtgtg atcgaagaag aggcagggat gtccattccg cagatcatgg
     1321 aacaacaagg ggaagggaca ttccgagacc ttgaaaccgc atcgctgaaa aaattggcgg
     1381 gcgaacagga aagtattgtg gcccttggcg gcggcgcttt attacgtgat gtaaaccgcg
     1441 cctttgcaga acaacatggc aggattattc ttttcatggc ggaattatcc accttgttgg
     1501 aaaggctgca ctatgaagct ggaaagcgcc ccctactggc aggagatttg aaaccgaaac
     1561 tgacagcctt gcttgaaaaa cggcgggagc actatcattc ctttccattg atgatccatg
     1621 tggatgggaa atctgccgaa caaaacgcct atcaggccca actggctgca ggcaggcatc
     1681 acctctcggc aatgggggag tatgatgcag tggttcaaag ccgcggaatt gaccagcttg
     1741 gcgaaatgct caaaacacgc ggattgcaaa atccgatcct tgttacggat gacaatgttg
     1801 cgaatctcca tgctgaaaaa gctgtcacgg ctttacggaa ttcaggtttc gatcccagag
     1861 tgttgaccgt ccctgcaggc gaggcttaca agaagctgga gaccatcagc cagatctgga
     1921 agggatttct cgaagcaggg ctggaccgca aaagcacggt cattgccctc ggcggcggtg
     1981 tcatcggcga tatgtcagga tttgccgcct ccacttacat gcgtggaatt gcatgggtgg
     2041 gtgtgccgac cactttgctt tcaatggtgg atgcctcgct tggcggaaaa acgggctttg
     2101 acctgcctga gggaaagaat ctcatcggcg cgttttatcc gccgaaactg gtactggcgg
     2161 acccacagct gcttgataca ttacccgaag ccgagttgat ctctggtatg gctgaagtgg
     2221 tcaagcatgg catcatctct gaccctgaat tgttctctct gtgtgggcgc gggttggatt
     2281 gggtgaaggc caacctcgaa gaagtggtca ggcgtgggat ggcggtgaag atcaaagtca
     2341 ttgaggaaga cccgtatgaa aagggagtcc gcgcggcctt gaatttgggg catacggttg
     2401 gtcatgcagt ggagcttgtc agcaggttca atttgcggca tggcgaggca attgccattg
     2461 gcacagtggt tgaggcgaga tactcggctc aaattggcag agcaggccac gggttggtgg
     2521 atgcggtgac ttcatccttc aagtcacttg gcttgcctgt gcaaattcca gatgccatgc
     2581 cgcgtgacga gatcatccgc gtgatgcggg tggataaaaa gaaaactgca aaggcgatcc
     2641 gttttgcgct gcctgttgaa attggtaaag ttgaactggt gaatgtggac gatttggaat
     2701 ctgttttgga gtaaagatga aaatattgat cttgcatggt cccaacctga atttgctcgg
     2761 cacgcgtgag cctgaagtgt acggctcaac gaagttgcac gaactcaatg aaagcttgat
     2821 cgcgcttgga aaagaattca atgcagaagt gacctgcctg caatcgaatc atgaaggtgc
     2881 gctgatcgat gccctgcatg aagcacggac acaggcgaat ggcgtggtgt tcaaccctgg
     2941 cggttacact cacacatcgg tggcgctgcg cgatgcgatc tctgccattc aaattcctgt
     3001 gatcgaggtg catatttcga atgtgcatgc acgtgaagag tttcggcata aatccatgat
     3061 ttcagcggtg tgcaaaggta ctgtctctgg ttttggtttg aagtcttatg aattggggtt
     3121 acgcgggctg attgatataa tcaggtcgtg aacactcaaa tcattccaac aactgaacct
     3181 ttttttcttc ctggcgggcg aacgggggta ttgctgatcc acggctttac aggcgcgccc
     3241 aaggaaatgc gctggatggg ggaatatctt aatgggcagg gatacacctg tttggggatt
     3301 cgactccacg gtcatgcaac aacacccgaa gatatgatcg cctcccgctg gaccgattgg
     3361 actgcttctg ttgaagatgg ttttcatcta ctctcgggca gtgtcgaccg aatcttcttt
     3421 gcagggcttt cgatgggggg agtattgtct ctgctgatgt ccaccaaatt tgcggatcgt
     3481 gtttcggggg tggtggccat gtctacgccg tattcgctgc ccaatgaccc aaaggactat
     3541 tcgcccgcat acatcagtct gtacagtcat tttgtaaaat acatgcccaa atcgggtgaa
     3601 gtgcctggcg cttcatggtt tgacaaggaa gccttcaaag accacatttc atatccgcag
     3661 aatcccatcc gctcgattgc ggagttgaag ctgttgctcg gtgaaatgca ggcggctctt
     3721 ccacgtttga ccatgcccgt tttgctgatt cattccaaag atgatccgta cgtgctgcct
     3781 gaaaacgtgg agaaaattta tgctgggctg gtcaatgctt cagacaagac aaagcttctc
     3841 gtgactgggt caggccatgt ggttacgcgg gatgcggccc gtcatcaagt gtttgaagcg
     3901 gcgcgggatt ttatccgcag agttgaaaat cagtcgtaat tccaagagga aactttgaat
     3961 cgaaatctga cgtttgtggt ggtgtccctt ttgttttggg gaattggcga agggatgttc
     4021 ttgaattttg tcccgatcta tctgggtaag gagtttgcgt tgggggagga gcagatcggg
     4081 ctggctcttg ggatgtttgg ttttttcagc gcgatcacac acatgccaag cggctatctt
     4141 gctgaccgca tcggcaggcg gcctttgctg atcacagcat ggatcctggg tcttgcttcc
     4201 acatttacca tgggaatttc aaccgctctg cctttttatc tggtgggctt gtttggctat
     4261 ggcctgactg cttttgtctc ttcgccgttg agcagttatg tcactgcggc acgaggcgag
     4321 atgccagtgg gcactgcgct ttcgctggtg tcggcttcgt tcaatatcgg catggcgctc
     4381 ggccctgtga caggcggttg gatcggtgac cattacggca tgcgcatgag ttatctggtg
     4441 gcggcagtga tttttctgtt ttccgttgcg agtatttttt tcatcaagaa tcaaccgctt
     4501 gataaacatg accccgaggc tccacccgtt actttgtgga agaaccgcgg cttcatttcg
     4561 tttttaggca tggttgggtt tgcagtattt gccatgtatc tggcacaacc gctcacgccg
     4621 aattttctgg aaggtgtgcg caaattctca ctgagtgata cagggtggat tttttcagca
     4681 ggcgcgttgg gaaatgcctt gttcgccatc ggtttcagcc gccttgatcc gcgcagggga
     4741 tttttgttcg cgcaggttct ggtggtgctt tttgcattga tcatgtggaa gggtttcggc
     4801 ctgcccctgt tcatgctggg gtattttctt ctgggtggtt tccgtgcggc tcgcccaatg
     4861 gcacaggcgc aggggcgtga acttgtgcat cagtcgcaga tgggtttgat gtacggcacg
     4921 cttgaaaccg tcaatgcgac catttttatt ttcacgccgc cgttggctgg ttatcttttt
     4981 gtaattgacc cgtttttgat ctatccgctg gcgatgggtt tgattacgct ggcgatgata
     5041 tctacattta ttttttccta tcgaaaggtg caacatgctt gagatcgttt cctttaccct
     5101 cggccccgca caaaccaatg cctatctggt ggctgaccct gacacaaaag aagccgtggt
     5161 gatcgatcca tcgtgggatg ggcatatcat tctggcagag gcgcaaaaac gcggctggcg
     5221 tatcggtcat ctttggtaca cacatgcaca ttttgaccat atcggcggcg cggctgcgat
     5281 tgccgatgcc ttgaatccgc tgccgcatgt ggccctgcat cccgatgacc atgtcctctg
     5341 gcgggcgggg ggcggcgggg caaagttcgg cttgaatatt gaccctggcc ccgagccaac
     5401 cattgatttt gtgcaaggca tgctcctcaa actcggttcg aacgagttcg aagtgcgtta
     5461 tacgcccggg cataccaaag gtcattgcgt tttatatgtt gccagcgaaa atatctgctt
     5521 ttgcggtgat gtgatcttca atggcagcat tggccgcaca gacctgcccg gcggcgattt
     5581 tgccacgctg gaaaatagca tccatactca gatttacaca ctgcccgatg aaacccgcct
     5641 gctttctggt catggacctg aaacgacagt ggggcacgaa aaaatgtaca atccctttgt
     5701 aagaactggg tgattccctt ataatttgtc caatcaacag gaggtaaaaa tgaaaaatcc
     5761 aaatgctcaa aaccaaaaaa cgaattctgc tccatcaaca ccaccttcca atcccaatcc
     5821 gaggccttca ttcttctctg tgatccctgg ttgggtggtc ggtttggtgg tggcggttat
     5881 ttttgttgcc ttgcttgcca gtgtgagcaa gtctttctgg tactttgaag tgatcgagaa
     5941 cgatcaagtt ggcattacga tcgaggcggg tgaaattcag agcgtgctgc aacctggcat
     6001 tgcctacgat ttcggcctgt ttgtggatct ggtcaagatc accaccagcg cggttgcgat
     6061 cactgtggat gaccctgaat tgatcacgat cgacaaacag cgcattgggc tggaggtcac
     6121 cgcggatgtg tttcgtccac gtgaagcgga tattgtcatc tctaattatt cgcgttatcg
     6181 caacatctat ctgaatgacg aatcgcttca acagcgtatg actgctttta ccctgcaagc
     6241 aatgaaggtt tgtgtgggtg ataagaaatt tgatgaagca gtcattggat cggggcgtga
     6301 tgatctgcga gcctgtattg atgatgaatt gagcgggctg gccgaaccgc tgggccttga
     6361 agtgcgcaat gtggcggtgc cgcaaataac gatttcgccc gaagtacagg cgggtttgga
     6421 tgccatcgtc caaagccgtt tagccactga aaaagccaag caggatgccg agaaagcaaa
     6481 acaggaagcc attgcccaac aggcagttga agaaggtcgc attcgggtgg atcaagccaa
     6541 attgcaggaa gaggccagac agcaagccat tttgcaggat atcaagcggc aacaactgga
     6601 agctgaactt gccgtaatcg agcaggaaca aatcaatgca caagccaaat tggaactgac
     6661 cgatcttttg caacaggttg ccgatgaaca ggctttggtg gatattgcca aggaattggc
     6721 tctggctgca ttgtatgaaa aatatccgtc gttcgtaact ttgcagatcg cccttgcaaa
     6781 tgcctctgcc atcaaaccca cagataaatt catcttcaca caggaaggcg tcttcccgaa
     6841 tttgattttc tctaacggta ccctgcctac attcaacgtg aacccataat cgtttggggt
     6901 ggagtttctc caccccaata ttttttcaga gtaaaaaatg aaacgagctg ttggcatcag
     6961 ccttggaagt tcgaaacgtg acaaaaaggt cactgtccgt ctcaatcaag ttgagattca
     7021 agtcgagcgc atcggcaccg atggcgatgt ggaaaaggca cgccagatgt atcttgacct
     7081 cgatggcaaa gtggatgcct tcggtgtcgg tggtgtggat ctatatctgc ggcttgacca
     7141 aaaggagtat ccgcttcatg ccgcgctgaa acttgtttct ggcgtcaagc agactccact
     7201 ctgtgacggt cgcggcctca agcacaccct tgaacggcgc gtgtttcaac tggcgagatc
     7261 cacactggga gagatccact ttaaacaggc gttcgtccct gtggcggtgg atcgactcgg
     7321 gttggctgag gctgttgccg aggtcagcga aagaaccgtg ttcggtgatt tgatggttgc
     7381 gctcggggtc ccgattcccg tttatgggat tcccgccttc aaacgagtcg caagggtcat
     7441 gctgcccatg gtcagtcact ttccgatgag catgatcttt tacggcagtg acggcgcgga
     7501 acatgagcca aagtacgtga aatattttga agaatcggac ctgatcgcgg gcgattttct
     7561 tttcatgcga aaatacatgc cgaaaagcct cgcaggaaaa accgtggtga cgaataccac
     7621 caccgaagag aatattgccc tgctcaaaga gcgcggcgtg aagacggtca tcacaacaac
     7681 gccgcgttat gacggccgat cctttggcac gaacaccacc gaagccatgc tcaccgccta
     7741 tgcaggcaaa ggacgcaggc tgaccgacga tgaattgaat ggtttgatcg atgaattggg
     7801 gttgaaacca aatgtgattc agttttaaac atgattccaa aaaaaactta aggaatcagt
     7861 cgcgaatttt gcaaaagtct gaaagatcaa ccactgagcc acgaagacac agagaaacct
     7921 cagtggttca aaacagcttt tcaaagacat tccttgcaac gtaacttatg caagaggtca
     7981 aatgaattgc aagacacaga cgagatataa aaagccgacc ctgtaaaatg gatcggcttt
     8041 tttgtgtttc taattcatcg tgtcgagata tttcaacccc gaccctgtgt tgaacatgac
     8101 gatcttttca tcgggctgaa cccatttttg cttgaccaat tcctcaaggg ccgccagaca
     8161 tgcagcacct tcaggggcgg cgaagatacc ttccatggcg gcgagttttt tctgggcccg
     8221 catcaaggct tcatcgctga cggctaccgc tgtgccgttg ctttcacgga tatttgagag
     8281 gatgatacgg tcggcgaagc tttttggcac acgcaagcca gatgccacag tttgggcacc
     8341 cagccaaaag tcacagaacg aagcgccagc atgaaaggcc ttgggaattg gggcgcagcc
     8401 ttccgcttgc accgaaacca tgcgcggacg ttttgtattt ttcagccagc caagggcttc
     8461 catttctgcg aaggctttcc acatgcccac cagacccgtg ccgccgccag tggggtagag
     8521 gatgacatcg ggcagagtcc agtcaaaaac ttcggcgagc tcataaccca tgatcttttt
     8581 gccttctgcg cgatacggtt ccttgaaagt ggaaacatca aaccaacctt cttcacgcgc
     8641 tttgacaccc gccataccag ccgcgtcact gatgaggcct tccacaagaa tgacctcggc
     8701 gcctgcaatg cggctttcct gaatgtttgc attgggtgtg tctttgggca tgtagatcat
     8761 tgcctgcaag ccagcacgcg cggcatatgc ggccatggct ccgcctgcat tgcctgcggt
     8821 ggggatgatg acctttttga cgccgagctc ttttgcacgg gatacggcgg cgcaaagtcc
     8881 acgggctttg aatgagcctg tggggttgct gctctcgtct ttgacgaaga aattggaaag
     8941 cccaagttca tttccaacgc gggggagacg tagcaagggc gtatctccct ctccgagagt
     9001 tacgatattt tcaggatcct gaatggggag catttcatgc cagcgccaca tcccttttga
     9061 gcgcgtgtga atctcgtcac ggtccacatt tgcgcgggcg gcattcagat cgtagtttga
     9121 aatcagtgta gattggcaat cagggcaaaa tgtaaggact tcattgatgg aaaagactct
     9181 tccacaatcc gaacaagtca gatcggtaag ataggatgtt ttcacaatat tcaacctgta
     9241 agtggtggtc ttgaattgtt tatttcgaat tgggggatgg aacaaatgct tcgacacgtg
     9301 cccaaagctc gcgaatgttg atgggtttgg acatgtacac atcgcagcct gcagagagtg
     9361 ccttttctgc atcacctttg agcgcattgg ctgttacggc aataatgggc acataagcca
     9421 gggcgtgttt tttgtcactg ctgcgaattc ggcgggctac ttcgtacccg tcaatatcgg
     9481 gcaggttgat atccaacagg attaaatcca cctcttctgt ttcagccttg ttgactccat
     9541 caagacccgt cagggcctgc accagtcggt atccgcgtga ttcaagggcg cgcctgacca
     9601 gcatcatgtt gtctggattg tcttcaatgt ataaaacgtt gtttcccatt ctcgtttgct
     9661 cctttaggcc ccgtttttat cttcgatgac gttgatgacc ttatccacga atttacgggt
     9721 gggtagtgaa cccttttgat atagcgcttc aatgcgtcca ttgaggcgct gacgttcttc
     9781 tgcagtgatg tcttttgcgc tgacgacaac aacaggaatt tccttcgtac gcgggtcaag
     9841 ttttagttct tccacaaagc caaacccgtc aataccgggc atggtcagat cactgatgat
     9901 caggtcgggc agatgctgcc gtgccatggc gagcccttcc cagccatcct tggcgtgata
     9961 aacacggtag gattttttac cctcaagcaa acggcggatg agcaaagcat cgtcggaatt
    10021 gtcatccaca agcagaaccg tggttgcttt ttcatccaga ttttcaagcg cggcttgcag
    10081 gccttcctgt ggagcagggg attggtcctt gctgaggtgg acatccacag cccattgatc
    10141 gccgaggaca tgtttttcaa cggggaaggt gtggcctgtg cagttgacga ccaccgtttc
    10201 atctttggtg atgatttttt cacgcagcat tttctcgagt ccggcgtagg cgacggctgt
    10261 tgcgggttcg acggccattc cctcactttt agcgagagaa cgcatggcgt cgaaggcttc
    10321 agcgtcggtc acagattcca tggtgccgcc gtgttttttg agaatgccat acaaataggt
    10381 ataggttttg cccgggtcac ctgtggagag gatgatgatg cgggtatcag gcaccacggg
    10441 gtcggccact tctttatcgg ccttgaaggc tttgaccatc ggtgaacagc cttcggcttg
    10501 aatgatcgcg atcttgggaa tgcggtcgat cagccccata tcgagcattt ccttgaagcc
    10561 ctgatacacc cccaacggcc ccatcccacc actgaccgct tgaatgtacc aatcaggcgc
    10621 gcgccagccg agttgttcca caatttcgta ggagatggtc ttcatcgatt cgcgggcggc
    10681 aatggaactt gcgccacggt caagatgcag gttgcggcgc tgtgcaaatt gcgatgcgat
    10741 ctgtttggtc tggtcgtaat tgccgctgac gcgaatcacc tccgcgccaa aaagggcggc
    10801 ctcgcgcaat ttttcctgcg gtaccaggct ggtcatgaaa acccaaagtt taatacccgc
    10861 ccgcgcacaa gccgcagcat aagccactgc ggcatttccc gtggaggcga tgaccgcttc
    10921 acggatgttg ttttcattca ttgccgcgac tgcaacggct gcctggcggt ctttgaagga
    10981 acttgtcggg ccgtagcgtt catccttgaa atacacccga tcatgcccca gcttggttgc
    11041 gaagcgctgt gaaagccaga gcggtgtgcc gcctgcgggg tagaggtcta aagcggaggg
    11101 attctcaagc ggcaggacat cctgaaaccg ccagagattt ctggggcggt ctggtaatcc
    11161 gcgcaggatg ttgcgtttga aaaccgcgta gtcgtatttt gcttcaagcc attgcgaccc
    11221 gcaggatttg caaacagcag ggacaaaagg ttcataaggt tgttggttgc cacaaatggc
    11281 acattgcagg aattgaggtt tggccatgcc gttattttac cttcaattct tcctcggttc
    11341 tgagccttgc ttcaatgggc aggaacacat agaaaatgga gccttcacca ttgatgccgc
    11401 tgctttcagc ccataatttt ccgccgtgca tttcgatcag gcgacggctg atgggcagcc
    11461 ccaaccccgt gccccctgct ttgcgggtgg tggaagtgtc gacttgtgta aattcctgga
    11521 agacgctatc gaggtgatcg tgcggaatgc cgatgcctgt atctttgacg ctgataagca
    11581 cgtggttatc ttcacacgcg gcacggatgg agattttgcc gcgttcggtg aacttgatgg
    11641 cattgttgac aaggttgatc atcacctgac ggatgcggat cttgtcggcg ctgatctgca
    11701 cttcgttgtc tgaatcctgt tcgaggaaga gcgatagatt cttttcgctg gcgaacgggg
    11761 cggtgatgct ggtcacttct tcgatgactt cctgcaggtt gaatttttcg atgttgaggt
    11821 tcaacttgcc tgattcgatc ttcgccatgt cgagcacgtc gttgatgagg tggagcagat
    11881 gttggccgtt cttttgaatg aggcccagat cgttgcgcat gttctctgtg agcggaccat
    11941 cgagttcttc gagcatcaca tctgaaaaac cgaggatgga gttgagcggg gtgcgcaatt
    12001 cgtgcgacat attggcaagg aaggatgatt tcaagcggtc caattcgcgc aattgcgcca
    12061 cggttgcggc ctgttccgca tacaaccgcg cattttgcag tgacactgcc acttgtgaag
    12121 ccagtgtggt gtagatgttg gcatcttctt ctgtaaagtg attgaggttt acagaatgga
    12181 cgttgaacac tcccagcacc ttatcgcctg cgatcatcgg gatcgccatt tcggagcggg
    12241 tatcgggcag caggggattg agttggaaac tttcctccct gcgcacatcg ttcgagatca
    12301 gggctttgcg ttcacgtgtg gcacgggaga caagcagttc attcttttcc atcggaatgg
    12361 tgttgccaga catcaccatc tgttttccga tatctcctga acctgcagcc aacaacaggg
    12421 tgttccaggc ttcgtcagca aggaagacct gtgcgtggta caagccgaaa cgttctttgg
    12481 tcaggtctac gacttgctgc aggagttcat ctggattcaa cacagtggat gctgcggtgg
    12541 atactgtggc caccgtggca agctctgcgg cgcgtttttg aatttcctct gtcaggcgca
    12601 ggcgttccag ttgttcactg acacgctcgg caatttgagc ggcaagccct tctgcttcag
    12661 gtgtgatgcg gtttggcgtc gctccgcctg catgggtctg tggagcgagt gttacaagcc
    12721 gacctatggt ttcgccgcgc accgtcaacg gctgtacaag gctgatgttt tcagcattgg
    12781 agaattcatc tgtcagcggg ctgacctgga tcgaatcata tacataaccg agggaagatt
    12841 gattgcgctg ggtgtacgat tgccagcttt cacggcttaa gcggcgggtg gcatcttcgg
    12901 catcggcacg ggcgcgggag gcatctgcca gcaggcgcag gttttcaacc tgttgcgaaa
    12961 cctggcgcgc gatcgcggcg accatttcct tatcttcctc gctcaatggg cgggcggggt
    13021 ccgatttgat gaacaggttt ccgatctgtt catcaagcac tttcacggtt tccctgtggt
    13081 catgctcggg aatatcacga acaggctcca ctgcagattg atcgtacaga tatccaatgc
    13141 gttcgctgtt gcgaatgcca tccaaaaacg attcccaact ttcatgggta aaacgacggg
    13201 tggcagcctg tgcttcggtg cgtgcgcgtt ctgtcgctga aagcaggcgg aggttttgga
    13261 tctggagtga tgcctgctgg gcaacggcgc tggcgaggcg ttcttcctct tccgtccacg
    13321 ggcggtcact gggcggctcg atcttgatat cgccaatcac atctccgccc atgatcaacg
    13381 gggcatccac tgcgtcttca agttctttcg ctgcgatctg tgcatctcga agcggcagga
    13441 tgttctcatc cttgaaggca tacccaactt tttctttttc atacacaatg gcagggatgc
    13501 gggccgtttc tgttttctct gtggcaagca tggcctcagc catggcaatg cgcagattgc
    13561 tctcttcgag ttttgcttcc aactcatgga tgcgtgcagt caatgcggtt accacaggat
    13621 caacgggcgg cgcttcctgt cggacgggga aactggtcgg gcgtgctgcg gcagaatccc
    13681 cgctggaagg gactgcctgc cccgcgatct gttccagatc cgagaagagc ttttccaccc
    13741 gcttttgtga tttatcttca gggtgcatac tcgactcctg tgctggggcc atttactttt
    13801 ctttatcctt catgctcagt tgagcaatgg tcatgggggc gccgagggcg tgccccaatt
    13861 cacgggcggc gatctgaagc acggcatcca ctgtggttgc gctttggatc ttctggctga
    13921 tgaggttcaa ggtactttca cgttcagcct gttgttgtgc gcggtcgaat tggcgtctgt
    13981 tttccaacac ggtggcaatt tgcggcgcca gcgaggagaa cagacggatt tcatcctgac
    14041 tgaaatggta gggctgttct gcttcaagca tgagtacacc cacctgacgg gtaccgatgt
    14101 aaagcggcag aaccgccacc gcacgcagat tcaattgttt ggcaagctgc aatgtggctg
    14161 catcggcacg ttcatcattg aatgcatctg caaaaaagag cggtaccggg ctggcaaaga
    14221 gtgaaaccgt gcggaagact gccacaggat aacgcacacc aatggcagtg acattgtggc
    14281 ctgtaccatt ccaccaattt gcttcgatgg acatgctctc cagtttatcg tctgctccgt
    14341 agttgaagat tcccaataca gcgcggttga caactggaat atcaagcgat tctaccgtgg
    14401 cggctaccag gtcttgcagg ctggtcgcct gggtcaggcg gcggctggca tcaaacagtt
    14461 tttctgattg ttcgagcgcc gattgtgcct gatcgtattg cctcaaagtt tcaatatggg
    14521 agctgagacg atccgcaaca gcggcggcaa ggctggctga gtctccatct tccagattca
    14581 ttccctgaat ggccagttcg ccgatcaact catcacggac tttaagcgga atggaaagtg
    14641 caggttctga tgtgctgtct ccattcagcg ggcgtacttc cttgaggtcg tatagatagc
    14701 tcaggttgtt tttcgcaccc tctttatagg ttttccaacc ttcgcggatc aggcggcggg
    14761 aggcttcttc agcctcaacg cggtagcgtt cggcattttc gagcaggcgc aggttttcaa
    14821 tttgttgggc aacctggcgt gccacaatgt tgatcaattc atttggacgg gtaagcggag
    14881 actggccttc caattccacc accaggtttc caatggtttc tccagttatg gcgatcgggg
    14941 cagtgagtgc atcatcgttc actgtttgat cctctgcctc aaccattggc atgaccttgt
    15001 tctgttcaaa tacataacca gactcttcgg gcttgtggat ggcatccaga tagcttgccc
    15061 aatttgtgcg ggtaaggcgc tgggcctgcg cttccacttc ggcacgtgcc tgctcggctt
    15121 ctgccagcag gtttgcattt tgaatggcga tggccaattg accagccaat gcttcaaatg
    15181 cggataacat ttcgtgattc aatgcacctg ctgtttcgct ctgcatatcc aacacgccca
    15241 ccactgtttc accaaggatc aaggggacgg ccatttcaga gcgggtgttc ggtagaaggg
    15301 gattggggcg gaaggtagtg ctggtggatg tatcagcgat gatcaccgat ctcttttcga
    15361 ccgctgcgcg accgttgatc gagcctgtgt tgatgggcag gcggtgattg cgagccagca
    15421 attcctctcc cacgtggcca gtaccagcct gcaggttgag gtaggttttg ctggggtttg
    15481 tcagatatac ctgaacatag taaagatcaa acctggagcg gatgatctca gccgcatcct
    15541 ttaacatcac atccagcttt cgaagctgcg atacagagcg accgacttgt gaggccagtt
    15601 ccaggttttg tgtacgttct gccacgcgtt cttccagcgt gacgaatgat tcgcgcaatt
    15661 gggtgatcat caaattgatg gtgctggcga gcagacctgt ttcatcggcg gcctgcactc
    15721 ttgcagtgac gttcaggtta ccctgcgaaa ttgcatttgc aacagttgta agttcgcgta
    15781 ccgggcgtac gatctggccg cttaagaacc atagaatagc aagggcggtt gaaatgagca
    15841 caagaccgat cacaccctga cgaatggcgg ctgtgttggc tggggctatg atctctgaaa
    15901 atggaacaag cagcacaaac gaccagtgat cgcccgtttg gccgaggtca accgccgcga
    15961 atgcgcgtaa agaatccccg tccgggctca ggctgatgaa ggattcacct gcttgaatgc
    16021 gtgactgtaa ctccaggaaa tcgggaaata tttctgcggc gggttggttc accaattcag
    16081 gctggttgcg gacgctgatc agggtgccgt ctgatgtcat taagactgca ccagctttcc
    16141 cgttgtaaag atcgacctgg tccaccagat tttgtacaaa ggcgatcggg gcatccacac
    16201 ctgcgatgcc gtagaattta tcagcataaa taattggtac gacaaaagat gccattaccg
    16261 tatccacgcc ttgaattggg tagatgagcg gggcaatggt cacttctttt tgggtttggc
    16321 ggggtatcag gtaccaatca cccaagcctg gggtttcgta atccaacaga gccgttacgg
    16381 tcaggcttcc atcatcgccg cggacccagt aggggatgaa gcggcctgta ttgtcatggg
    16441 ctgcggtgcc tttgaaggtt tgatcgagcc cgtcaaatgc gtttggttcc cagagggtgt
    16501 aggttcccaa aaagtttgga ttctcaagaa ggacctggcg cagcatggcg ttgacttgag
    16561 tgcggcttag aacttcatca ttggatggtt ccttggtgga ggtcagcgca tgtgcgagcg
    16621 cacgtgctgt gtccaaggga atttcaacat ctgcacgcac tgaacgtgct tgtgattcag
    16681 cgaaggacaa cgcttctttt tctgccgcat caacggcagc ctgacgggca atgatttgag
    16741 agtaaacgat caggatgatg gaaatacctg tcaggatcag gcctgtccag atggtgatct
    16801 tgaggcgaag attggaggaa atgacattct gtgattctct aaatatgtag atcgcaaaga
    16861 gaatgacaaa aatacctgcc cctgccgggc caacctgggc catgttcatg gacagacgcc
    16921 aggccgggtt tagcaattca gtcacccaga tcgccacaag tgaaacgatc atgatggttg
    16981 catattcacg gcgggtatgc tgcgggaata gccagatgaa accaacggtt gaaatgctca
    17041 gaatggaaaa agtaatcgag gttgtgcggc cttgaaataa aacgactgca gcaacacata
    17101 atgcgatcaa cgagtaatag gtcagcttca gacccagttc tgtgcgctga cgaatggaaa
    17161 ggaccagacc catcacagta gatgttgaga ccagcaggag gaccgccatg gcaatgtaac
    17221 tttgggtggt cttttctcca ataaatattt gtgtcaggac ataggcgtac accaacacca
    17281 atatggttga caggagaatt gtgccccaac gtgtgtgttg gcggctttca tcgtccagat
    17341 ggagcggata tgtttttttg ttatccattt ttgtttctcc ttcatcgggt agttcaggtc
    17401 ttcttcccga tgcggttcct tgttggcaaa tatcaaaagg aggctttttt attttttgtt
    17461 atcgctgtta ttacgggcgg agatcgctgc tttgacgcgg gttgcaccca atgcaagccc
    17521 aagttcgcgg atggcggttt gcagggtttc ttccacggtg tttgcgcgtt ggatcttctg
    17581 gccaatggta tttaccagcg tttccaactc ggcctgtgat ttggattgtt cgtacgagcg
    17641 tgcgttttgt aaagagatcg cgacctgccc tgcgagggat tgtagtaatc tgacgtcttc
    17701 ctggtcgaga ccattaaccg tattttgctg cacatccaac acgcccagaa cctgcctgcc
    17761 cgaggagatc ggaatggcta cttcagacct ggtatccggc agcaaggggt tggggagcca
    17821 gccttcggct tttgaaacat caggtaccaa taccggggca ttggtggcag cagcacggcc
    17881 caccaagccg cgtccatgag gaattttgtg accacgtgca agcatggctg cgcctgcatc
    17941 gccggtaccg cctgccatga tgaggtctcc gctttcttca tcgaggaaat aaatatgggc
    18001 gtgatagtat ttgaatgcgg cctgcacttg ttccaccact tcaacggcaa ggcgttgtgg
    18061 gtcggtggca ctggaaaggc gttgactgac ctctgcggaa gtggataatg ctttggtacg
    18121 catttcgaca cgtttttcca tatcttgcaa agcgttttgc agttgtgaag tcatggtatt
    18181 gaaggcatct gccagaacgc cgatctcatc attgctttgg attttcgcgc gttttgttaa
    18241 gtcaccctct gcaattgcct tggatgtaat agtgagatca ctgattgggg taagggtacg
    18301 gctggccaga aacgagaagc caaaaccaat tgtcaaaagc attaccacca agatgccaat
    18361 gtagaacatg acctgacggg tttgctgttg agcaaccgca gtatggtctg aaataatatc
    18421 caccaccccg atcactttgc cggaaaaatc atatagaggc gcggtcaaga ctgcatagct
    18481 gtgcccctga tagtcaaagt gttccagtgt cgcactccct gcaagtgcct gttcgtaatt
    18541 tgttattggc ccaaagaata ctgtttcgag tgtgctggcc tgaagaatca gactgtcaat
    18601 tggtccctgg ctttttcctt ctacgcctgt gaatgtggca atttcagcag catttcgctc
    18661 caaaaggatt tgtagattag cccctgattt tgtgtgtaat gcttcaagca aggttaagtc
    18721 aacgttgggg ccaaattcaa cggtcccgat gtgccgtcct tgataagtaa ccggaacgac
    18781 acctcgaata cccaaaccgc ctctcccgat ttcgattccg ctgactgctc ttttttcctg
    18841 attggcaatc aataccgtct gacgaaaact ggagagatcg tcaccatact gatcaaggct
    18901 atgtaagcga agaaaagatg tggcaggaga aagatggaac tgatattgtg ggatgtcaaa
    18961 ttgggcatca atcacttgat aggaaggcag tgtcaatgcc gccagtcttt cacggtctct
    19021 ggcggcaaat gcagcttgaa tttcgggatt attcgccacc tcataagcca aagcgattgc
    19081 cagatcttcc ttggttttaa tttctgtcag aaattccttg tgaatatctt ccagatgatt
    19141 ggcttcagat tcatcgaatt gccgtactgc agtaatcgaa aaatatgtaa acagcccaat
    19201 cagggttatg ctgataaaaa ttaaaatggg ggtgagtagt tttctggtta atcgcatagg
    19261 attctcctgc tcaattcgag gttgcttgat aaaggcgaga tgaaatcagc ggcggattgc
    19321 tcaagataaa gatgaaggtt ccgcgctgct gttgggggct gtatccaacc ttacgattgt
    19381 ggatttcatg cccatggcat gccccaattc acgtgctgca acctgcaagg ca
//
LOCUS       DAOQLC010000082        18954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1011_length_18954_cov_19.913964, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000082 DAOQLC010000000
VERSION     DAOQLC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_18954_cov_19.913964"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1478)
                     /locus_tag="PL000_14130"
     CDS             complement(75..1478)
                     /locus_tag="PL000_14130"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HNC90089.1"
                     /translation="MKNQLPEGVTWRRVVEVHEADSFLYNLSIACNHCMHPKCAGVCP
                     TDAYFVRNDGIVSIDPLKCMGCGYCAWACPYNAPRYNPTQGQMTKCNFCADSLDAGLP
                     PACVAACPQQALGYEKIDGERAVPNENHWLDLWETPANAHPFPLPTRSRTRPHLAITP
                     TPTMTLPTETRSYNHEELKPKNIKSELPLVSFTLLIQMAVGGFWSLLSWSLLTPNSID
                     GKLTFTSTLISILLATLGLASAFLHLGKPKNAWRSVQHWKKSWLSREILFTASFVIFG
                     LVTAHQSGIAYREIPMLYSWYGITHWSLVHPCFTGELSCNFILPSAATVQVWMLVTSI
                     LGAASVYSMARVYRLHIIAAWDTWRTTAGFFVTTALLGQATILVLAFIGNTQLNWGII
                     TALLVIQLGLWLQTPHQERGHIARAVLIVSALCGAAALTMTGNHFWLGVPVIFSILIE
                     ETLGRKVFYQALTDKAL"
     gene            1696..4155
                     /locus_tag="PL000_14135"
     CDS             1696..4155
                     /locus_tag="PL000_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC90090.1"
                     /translation="MEQVLPSQARVVIIGGGVIGCSTAYHFAKLGWTDVIVLERKQLT
                     SGTTWHAAGLVVCGGFYTETMLELAHYTRELYRNLEKETGISTGFKPVGYVQAASSAS
                     RLRFLRKNAAFARSLGIEAQEISPSQFKDLWPLADPAGVTAGFYFPDDGRANPVDVTM
                     SLAKGARDLGVRIFENVEVDSVTKKMGRAVGVVTKTGESISAEYVINCTGMWGRKVGA
                     QAGVSIPLQAAEHYYLLTEPIEGVHANLPILEDVDTYSYFREEVGGLMLGLFEPVAKP
                     WGVNGIPENFAFGEIQPDWDRMMPYIEAAMERVPVLKTAGIRKLFCGPESFTADMTML
                     VGEAPELKNFWVAAGMNSLGILLGGGVGHTLAHWIVDGVPPLDVGEISLDRMLPYQNN
                     QRYLNDRIVEILGMMYKETFPNSQLQTARNVRKSILHEKLEKHGAFFASSAGWEYADW
                     FAPEGREARVEKYSWDRQNWFEYNAAEHKACREDVILMDTSMMSKFLVQGRDAQKMLD
                     RISVSNINVPIGKIVYTEWLNARGGIEADLTVTRIGEESFMVVCSDLAHRHVETWMQR
                     HTETGEQVFIVDMTSAYALINVQGPKSRELLSRLSSADLSNEAFPYMSMKEIDVHYAR
                     ALAFRVTYQGELGYELYIPTEFAPTTFDALLEAGKDLGVKLAGMQALNTLRIEKGYRD
                     YGHDIDGTDTPLEAGLSFLVDFNKPNGFIGKEALLKQKEAGALKKRFVQLILDDPEPL
                     LYYGEMIYRNGKIVGYVRSGAYGFTLGGAVGLSIIEDDEPINEEYIKNGRFEVEVNNV
                     MYSAKASLRPLYDPKNERVKS"
     gene            4359..6056
                     /locus_tag="PL000_14140"
     CDS             4359..6056
                     /locus_tag="PL000_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC90091.1"
                     /translation="MYRVKLTSLFFIIILTATSLFGCQQNAPADSAQNSITIVIPEDP
                     PSFNPIIADTGYDALVMELVMLGLSDIDPEGKVFPELAAELPTLENGDVVVDDDAGTM
                     TVTWKMREDVQWADGTPVTADDVIFTYESIINPETGGWIPGIDYIDSVEKLDDHSFTI
                     SYNSIYPGYLTQFGGEQVVIWPAHYCDASQGFASWECARKPLSDGPFVLTEWVNGDHM
                     TFVKNEKYFEQGKPGIDAVTVRIIPDQSVRKTMLINGDADLDMWTNEQVVSDLQGEAN
                     VSVDPSPTNRWVMRIFFNLAAKGTTDPVATPHPILSDLRVRQAIRAAIDVDTISKEFF
                     LGYAKPAWTEFFRPPYTCDIPRPKFDPAAASALLEEAGWKDTDGDGVRECRGCSTAEE
                     GYKMEMEFITYSEYGEPLELTQQYIAEQLGNIGIKLNLTIVEGSVLWAASTDGGIEQS
                     GNFDMDIWDDGYSGVDPTDFLWSYYSTDAAVPDAGYNYSRWSNEQFDTLLGNVYTLDE
                     GQRQKDFCEMAKLLDAEIPQLLLFTASNADAHNARLQNVQSSTNDLVTWNSADWVLK"
     gene            6058..7101
                     /locus_tag="PL000_14145"
     CDS             6058..7101
                     /locus_tag="PL000_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90092.1"
                     /translation="MATYIFRRLLQTAGLLALLSVLLFALVNLAPGGPLAGQGQSRHI
                     DPDKAELLKRQFGLDKPLPTQYLVWLVGNDWMQVDKDGDGVLDSYGTRQGILRGDFGF
                     SFRTRQPVLTEIGQRLPNTIYLMTVTMGVAVLIALPIGIISAIRQYSTFDITVTTFSF
                     AGQAIPEFWLGLILILIFYAWLKNPFTGEPLLPPGGMTSSNSTGFDLGDRVIHLILPV
                     ATGALGWVAWYSRFLRSSMLEVIHQDYIRTARAKGLSNLKVLSKHALRNALIPLVTLL
                     ALDLPYIFGGAVFVEILFAWPGMGRLYYQAALDRDYPILLAVLIIGAGFIILSNLLAD
                     IVYAYLDPRIRFE"
     gene            7105..8055
                     /locus_tag="PL000_14150"
     CDS             7105..8055
                     /locus_tag="PL000_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90093.1"
                     /translation="MNRPESNHPLVSELQASAATFQSASMGAGVWKRFRRHPGAIFGF
                     AIIFVLAVLTLLAPLSPYDPEASDLSERNNPPSLSHPMGTDALGRDLLTRVLFGGRIS
                     LTVGALVVVISLLIGVPIGALAGYYGGVLDGILMRFTDSFLSLPSFLVLILLSAILRE
                     VDAPLFEKNNVLTISMVIGVFGWTTFARLVRATFLTLRETDFISASRALGGSDLRIIA
                     RHILPNAIGPIIVEATLEFGYAIIEESGLSFLGFGIQPPTPSWGNLLSNAQEHFTKYP
                     WLAIFPGLMIFLSIISINYIGDGLRDAFDPYKVLEKVGEV"
     gene            8109..9560
                     /locus_tag="PL000_14155"
     CDS             8109..9560
                     /locus_tag="PL000_14155"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC90094.1"
                     /translation="MSGNRPALEPLRSAFRIQYLTEQQLDTLQEATLDILENVGIKFP
                     SERALNVFIEHGADVDKNTQVVKIKRDMVFKAMKTVPRYFVMGARVPEYDLNLQEGTT
                     YFTTDGCGVEVVDLETRQYRPSSKADVGMMARVADYLPSIGFYWPMVSAQDCGRTSPL
                     HELEIGWNNTVKHFQSETIMGGREAAYAVEMATVITGSKEELRKRPPLSSLICTVSPL
                     MQDQPGIEAAMVFAEAGIPVGFLAMPTLGTTAPITQAGALAMADAEIISATVLMQLMF
                     PGAPIYHSMMQAWADMRTGGYVSYPRDARGRYGVVDMAHHWGMPSLGACYGTDSVVTG
                     TWQAAAEPALDPFLAGLASPEIVTGMGLSRTYTRLFPEQIILDDDLYQRARAYLQRME
                     VDEDSLAMEAIRNVGPGGHFLGQKHTRAHMKTSLVRGVTHELDAQNKYRDPVEVARER
                     VRWIIENHQPEPLEKSQQEELARIVAAADKELG"
     gene            9591..11027
                     /locus_tag="PL000_14160"
     CDS             9591..11027
                     /locus_tag="PL000_14160"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC90095.1"
                     /translation="METDLKVLSSNEIAQIHERTLSILATTGVRVDTAKGRKFLKKAG
                     AEVNATTNVVRFPRGMVEECLQLAPRSFSLGGRRPNWEFPLNANLCSLVADGGASFAI
                     DEQTGERREANFQDWLGATRMIDSLDEVGVYWSMVDPGFFQKSAGDYVKYLHQMVGNF
                     SKHLQDCTHTIEESRWMLEVLGTVFGGKENVRKLRPFSYLVTPISPMVLEEQHTDAYL
                     EVIDWGVPVAIMPMPMMGGTAPASLISTILQANCETLAMLCLVQAASPGTPIIYAASP
                     AIMDPYTGRFTGSEVEHGLMGAAITEVARSYGLPVETSAGSTGHYAPGVRASYERALN
                     WVMATLSWPDLLVGPGQLGGSLLLSLEQLLIDVEVFHRSSRLSSGIDTRVKEWLEVDL
                     AAVGHGGNFLSRRSTREALRNGEVYMTDYDARNSYEAWAASGKPTILDELKPKVRKIL
                     DTHSPLPLDEDVERELERIEQRAREMRG"
     gene            11032..12465
                     /locus_tag="PL000_14165"
     CDS             11032..12465
                     /locus_tag="PL000_14165"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC90096.1"
                     /translation="MRFQSQILSDTEKEQVHKEALQILQTTGAKFHSDKALKLLEDNG
                     ARVDREKRIAYIPPELVEQALASTPRSFVLGARNPKFDYPMPSPVTRFCLDGTGSFAQ
                     DFYTGEHRYGTLKDNENGLRVFQAMDMGVMAWPPTSAEDKPDYSRPLHEWAAMMKFCS
                     KHGEHELHFVNQTPYFAEMLVAVMGSEDEVRARHAYSLTYCPVSPLIHDGPMMDAYIE
                     MGSLDVPIMVLPMPAPGMTGPASLFGAICLADAEALSSLVTYQLAHPGRPMIFSSATG
                     TMDMRNGAFLGGTPEMGLMSAALIEMGRFYDLPSCSAGCTADAREPGADAVLEKVITT
                     IPPVLAGSDIVLGIGVVESDQLLVLEQIVVDNEIAHFCERIFQGVDSAPGRILTKDVQ
                     AVGPGGNFLSRKSTLEFARSKEVHFSDLLDRHTLDQWLQLGKPSMYSAARKKVEEILA
                     GPVLDPLSDETIGTLDEILIRADRELA"
     gene            12523..13977
                     /locus_tag="PL000_14170"
     CDS             12523..13977
                     /locus_tag="PL000_14170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HNC90097.1"
                     /translation="MRTSPFLLRSSQYVLAFVVIAFVTAVLFVLREALDTTLIALLYL
                     IPLGLVTAFLGFGPGIASALMTFLAFNYFFIKPYYTFAVHRPTDVVILVIFLVVAIVI
                     SQLVGRMQVSLAAATAREREATQLYELSTALAGLHDEQVIVQILARQVQAVLLGEAVT
                     VHIVGGQPFQFSVLETGQPQRPPEWLVPIEAARGVLGEIRVWRAGPVLTSSEKRLLQT
                     FASQGAVALERARLVQAESRARVLEESDHLKSILLSSVSHELRTPLSTIKAAASSLRS
                     NEINWESPARPELIAAIDEEADHLNILVGNLLDMSRIESGALKPKREWNILAEIVEGV
                     LARMKHLLDEHILEVDVPESLPLVPVDYVQIEQVFTNLLSNGVKYAPAKTLIRVRAFV
                     EDDFVHVVVGNQGPQIPREHLERIFEKFFRITAADRVTGTGLGLSICKGIIEVHGGRL
                     WAENVPDGLAFNFTLPLIWEGVPPPKLPMDTETE"
     gene            13974..14669
                     /locus_tag="PL000_14175"
     CDS             13974..14669
                     /locus_tag="PL000_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90098.1"
                     /translation="MSNSPHILVVDDELQIQRAIRTILTEKGFKVSTASSGNEGLTLA
                     AANVPDIVILDLGLPDMDGAEVCVRLREWTQCPIIILSVRDSERDKVSALDKGADDYL
                     TKPFGIEELLARVRVALRHAASQPGPQSKVVKAGQLTIDLAWHVVKLGEDEVKLTGTE
                     YKLLAYLAGNRGRVLTHQSILSHVWGPADADHTEYLRVYMRQLRKKLELDPERPRYIL
                     TEPGIGYRFIADE"
     gene            14798..15439
                     /locus_tag="PL000_14180"
     CDS             14798..15439
                     /locus_tag="PL000_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90099.1"
                     /translation="MKKVDLTPPVFALPPVSAVDLGTARRLIVLVPEIEMDIAYVARK
                     LGELTNSFNGCILFYGLCKDAVCELSLRRQLVALIAMMEAEKLSIESRIEIGENWPAS
                     VLTDLRQEDMVVCFPAPYPGFDRRPLHQFLSANLNCTVYMLDGVAHRETQSFAERASS
                     LMAWGGSIGLVFAFFWLQAKIVQLPQDWVHTSMLYFSLFAEVGAILGWNALFD"
     gene            15480..17444
                     /locus_tag="PL000_14185"
     CDS             15480..17444
                     /locus_tag="PL000_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HNC90100.1"
                     /translation="MINTNENKNSQTSIIELDIDYKPPRTLRSWLIGRPLSTADASHQ
                     TIGKVVGLAVFASDALSSTAYATQEILGVIAAAGTVAYGYLFPISLAIITLLAIVTIS
                     YEQTIHAYPGGGGAYIVARDNLGELPAQTAGAALLTDYILTVSVSISAGVAQIVSAYP
                     GLFPYRVAIAVIAVLLIMLVNLRGVKESGTAFAIPTYFFVVLMIATVGYGLFRYFTGS
                     LGMVIDAPHFEAHHVISVITPFILLHAFANGTTALTGVEAISNGITAFKEPRSKNAGI
                     TLIWMSLILGSLFLGLSFLTGKVHAVFSEEETVISQLARTIYDGRGIPYLLLISGTTV
                     ILIMAANTAFADFPRLGALHAGDGFLPRQLTYRGSRLVYSRGIVSLAVIASVLIIIFQ
                     ASVTRLIPLYAIGVFLSFTLSQAGMARRWWKIGHLKEGVEIVEPGSTLRYESGWQYRM
                     LINGFGALCTAVVMVVFAVTKFAEGAWVVLILTPILVAIFFAIHHHYKRLAKKLSLDN
                     FGAIPPHAIRHRVILPVSGVHQGTLAALRYARMLSDDVTAVHVTIEPAEAEKIRKKWE
                     TWGGGVRIVMLASPYRLFLEPLLGYISAISRQRQAGETITIVVPEFVSDGRVTALLHT
                     NTADLLRSQLKRQHGIVIINVPYHVHEWDG"
     gene            17617..18273
                     /locus_tag="PL000_14190"
     CDS             17617..18273
                     /locus_tag="PL000_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC90101.1"
                     /translation="MQEAIKETAWKQIAETGAAALSLRAIARELNITAPAIYNYFPDR
                     DALVTALIIDAYTSFGDYQIASRDSQAGDDLAGSLQAIGFAYRKWALEYPQRYQLIFG
                     TPIPGYQAPIMEVLPSAARSLSALVSVIDKLRIANELNAEGFPSIQPGYEQMFNVWRG
                     FAGDYDIFSLSVAMIIWSRVHGLVSLEISNNMPPFGVDGASLYRYEMESIVRQFIKGN
                     "
     gene            18276..18716
                     /locus_tag="PL000_14195"
     CDS             18276..18716
                     /locus_tag="PL000_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90102.1"
                     /translation="MYTVSITRLRVRSIFLMPLFTLHAMRTSSQAQKAEGILGVETRF
                     EKNNVVWTKTIWTEESAMKKYRGSGAHQIAMRILSEMCSEASVTRWQQESTELPTWEE
                     AHRRLLAEGKLSKVKHPSPLQASGKTAPETMQSGFKMPAPPVTQ"
     gene            18713..>18954
                     /locus_tag="PL000_14200"
     CDS             18713..>18954
                     /locus_tag="PL000_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC90103.1"
                     /translation="MNFAKSWMFNHPVTRRVLDGYFENDGIRQHYVTLGEGKLVVLIH
                     GFPEFWYSWRHQIEALSQDYQVVAIDQRGFNESGKPA"
BASE COUNT         4249 a         4570 c         5183 g         4952 t
ORIGIN      
        1 ctggagaatt acataagggt ggaggaattg tgtggaactt gtgtgttcaa aattgaagtg
       61 ctggcgaaaa caggctacaa cgccttgtct gtcagcgctt ggtaaaaaac ctttcgtcca
      121 agagtctctt cgatgagaat tgagaaaatg acaggcacac caagccaaaa gtgattgcct
      181 gtcattgtta atgccgccgc cccgcacaag gctgaaacga tcagcacagc tcgagcgata
      241 tgccctcttt cttggtgcgg agtttgtagc cacagcccca gctggatgac cagcaaggca
      301 gtgatgatgc cccaattcaa ttgggtattc ccgataaacg caagaaccaa tatggtggcc
      361 tgcccaagca aggcggtggt gacaaaaaag cccgccgtgg ttcgccatgt atcccatgcg
      421 gcgatgatgt gcagacgata aacccgagcc atgctgtaaa cgcttgcggc cccaagtatg
      481 gatgtgacaa gcatccatac ttgcacagtc gctgcactgg gaaggatgaa attgcaggaa
      541 agctcaccag tgaaacaagg atgtacaagg ctccaatggg tgattccgta ccatgaatac
      601 agcatgggga tttccctgta cgcgattccg ctttggtggg cagtgacaag cccgaatatg
      661 acaaaagatg cagtaaacag tatttcgcgg ctgagccatg attttttcca atgctgcaca
      721 cttctccacg cattttttgg tttgccgagg tggaggaagg cacttgccag cccaagcgtt
      781 gccaaaagga tggagatcag agttgaggtg aatgtcaatt ttccgtcaat gctgtttggg
      841 gtgagaaggc tccagctcaa tagactccaa aatccgccga ccgccatttg aattaaaaga
      901 gtaaatgaca caagcggcag ttcgctcttt atgttttttg gtttgagctc ttcgtggttg
      961 taacttcgcg tttccgttgg caaggtcatt gttggggtgg gagtgatggc aagatgcggg
     1021 cgtgtccgtg aacgagttgg cagtggaaag ggatgggcat ttgctggcgt ttcccataaa
     1081 tccagccagt gattttcgtt tgggacagct ctttcgccat caattttttc gtagcctaat
     1141 gcctgctgcg ggcaagccgc cacacatgca ggcggaagcc ctgcatccag actgtcggcg
     1201 cagaaattgc attttgtcat ctgaccctgt gtcgggttgt agcgaggcgc attgtaaggg
     1261 caggcccagg cgcaataacc acatcccatg catttgagcg gatcaatgct tacaattcca
     1321 tcgtttcgga caaaatatgc gtcggtcggg catacccctg cgcattttgg atgcatacaa
     1381 tgattgcatg caatggacag gttgtataaa aaagaatccg cctcatgcac ttccaccacg
     1441 cgccgccatg tgacaccttc agggagttga tttttcacct gacaggcttt cacgcaatct
     1501 cggcaacctt caatgcattt gcctgcgttg aaggtgaagg catggaatat ctgtgacttc
     1561 atgtggtaag tgtaaagtga aggcgtgtgg acaggaagtg atggatgtaa cttgtttttc
     1621 ctcttgcccc aaatataaaa ttcctgtatc cttggctgga cggtgaaatc catttcagca
     1681 acacaggagc ggtaaatgga acaggttctt ccttcacaag cacgggtggt gattattggc
     1741 ggcggggtga tcgggtgcag tacagcgtat cattttgcaa aacttggctg gacggatgtc
     1801 atcgtccttg aacgaaaaca actcacctct gggacaacat ggcatgcggc agggttggtg
     1861 gtgtgcggcg ggttttacac cgagaccatg ctcgaacttg cgcattacac ccgtgaactg
     1921 tatcgcaacc ttgaaaaaga aactggcatt tccacaggct tcaaacctgt tgggtatgtg
     1981 caggcggcaa gttccgcttc gcgtttgcgc tttcttcgaa agaacgccgc gtttgcccgt
     2041 tcgcttggaa tcgaggcgca ggaaatttcc ccgtcacagt tcaaagatct ttggccattg
     2101 gcagaccccg ctggtgtaac ggctggattt tatttccccg atgacgggcg cgccaacccc
     2161 gtggatgtga ccatgagcct ggccaaaggc gcccgtgatt tgggcgtgcg catctttgaa
     2221 aacgttgaag ttgattctgt tacgaagaaa atgggccgcg ccgttggtgt ggtgacgaag
     2281 acgggcgaat ccatttcagc cgaatatgtc attaactgca caggcatgtg gggacgtaaa
     2341 gtcggcgcac aggcgggggt gagcatccca ttgcaggctg ccgaacatta ttatttgctg
     2401 accgaaccca ttgaaggcgt ccatgccaac ctgcccatcc tcgaagatgt agacacatac
     2461 tcctacttcc gtgaagaagt gggtggattg atgctggggt tgtttgagcc cgttgccaaa
     2521 ccctggggcg tgaacggtat tcctgaaaac tttgcctttg gcgaaattca acccgattgg
     2581 gatcgtatga tgccttacat cgaagccgcc atggagcgtg tgcccgtgtt gaaaacagcg
     2641 ggcattcgta aactgttttg cggccccgaa agtttcaccg ccgatatgac catgctggtt
     2701 ggggaagccc ccgagttgaa aaatttctgg gtggcggcag gcatgaattc gctcggtatt
     2761 ttgcttggcg gcggggtcgg tcacaccctt gcgcattgga tcgtggatgg agttccgcca
     2821 ttggatgtcg gcgagatcag ccttgaccga atgctgccat atcaaaacaa ccaacgatat
     2881 ttgaacgacc gcatcgtgga aatccttggc atgatgtaca aggagacttt cccaaactcg
     2941 cagctgcaaa ctgcgcgcaa cgtgcgaaaa tccattttgc atgaaaagct ggaaaaacat
     3001 ggcgcttttt ttgcttcttc tgcaggctgg gaatatgcag attggtttgc tcctgaaggc
     3061 agggaagcca gggttgaaaa atacagttgg gacaggcaaa actggttcga atacaacgcc
     3121 gccgaacaca aagcctgccg cgaagatgtg attctcatgg acacttcgat gatgtccaaa
     3181 tttctggtgc aggggcgcga tgcgcaaaaa atgctcgatc ggatctccgt cagcaatatc
     3241 aatgtgccga tcggaaagat cgtctatacc gagtggttga atgcccgtgg cggcatcgaa
     3301 gcggacctga ccgttacccg cattggcgaa gaatccttca tggtcgtttg ctctgacctt
     3361 gcacatcgcc atgtggaaac atggatgcag cggcacactg aaacagggga acaggtcttc
     3421 attgtggata tgacttcagc gtacgccttg atcaacgtgc agggaccaaa atcacgcgaa
     3481 ttgctcagcc gtctttccag tgcagatcta tcgaacgagg cctttcccta catgtccatg
     3541 aaagaaattg acgtgcatta cgcacgcgca ttggccttcc gtgtgacgta tcagggcgag
     3601 ttggggtacg agttgtacat ccccaccgag tttgctccga cgaccttcga tgcgcttctc
     3661 gaagcgggga aggatttggg tgtaaaactg gcggggatgc aggcactcaa tacccttcgc
     3721 attgaaaaag gctatcgcga ttacggccat gatatcgacg gcaccgatac accgcttgaa
     3781 gcgggtttga gtttccttgt ggatttcaac aagccgaatg gattcatcgg taaagaggcg
     3841 ctgttgaaac aaaaagaagc gggtgcgctc aagaagcgtt ttgtgcaatt gattttggac
     3901 gaccccgaac cattgttgta ttatggcgaa atgatctatc gcaacgggaa gatcgtgggg
     3961 tatgtccgct cgggggctta tggcttcacg ttgggtggtg cggtgggttt gtccattatc
     4021 gaagatgatg agccgatcaa tgaggagtac atcaagaatg gcaggtttga ggttgaggtg
     4081 aataatgtga tgtattctgc aaaagcgtca ttgcgcccgc tgtatgaccc gaagaatgaa
     4141 agagtgaagt cctgaggaat gcggacttaa gtccgcgttt tcggggagag aatttttttg
     4201 acgctgaggc aatcatcggc tgggcgggat gcgggttcat atccgcgaaa cagagatgaa
     4261 aatcacatga catattgggt aaagtatgca ataattttgg gaagtaaagt acggcacata
     4321 taatcgtaat aacaaatcca aggtaaatcg gaggagagat gtacagagta aaacttacat
     4381 cgttgttttt tattattata ttgacggcga ccagcctgtt tgggtgccag cagaatgctc
     4441 ctgccgattc ggcgcagaat agcattacga tcgtcattcc tgaagacccg ccttctttca
     4501 accccatcat cgctgatact gggtatgatg cccttgtcat ggaattggtg atgttaggtt
     4561 tgagtgacat tgaccctgag ggcaaggtct tccccgagct ggcggcggaa ttgccgaccc
     4621 ttgaaaatgg cgatgtggtt gttgatgacg atgcggggac aatgaccgtg acatggaaga
     4681 tgcgcgagga tgtgcagtgg gcggatggga ctcctgttac ggctgacgat gtgatcttta
     4741 cctacgaatc gatcattaat cccgaaactg gcggctggat cccaggcatt gattacattg
     4801 acagtgtgga gaagttggac gatcactcgt tcacgatttc ctacaacagc atttacccag
     4861 gctacttgac acagtttggc ggggaacagg ttgtgatctg gcctgcgcat tattgcgatg
     4921 cctcacaggg gtttgcttca tgggagtgtg cccgcaagcc attgagcgat ggcccattcg
     4981 tgttgaccga gtgggtgaac ggcgaccaca tgacgtttgt gaaaaacgag aaatattttg
     5041 agcagggcaa gccagggatt gacgcggtta cagtgcggat catccccgat caatcggtgc
     5101 gcaagaccat gctcattaac ggcgatgccg atctggatat gtggacgaat gagcaggtgg
     5161 tttcagacct gcagggtgag gccaatgtga gcgtggaccc cagccccacg aatcgctggg
     5221 tgatgcgtat ctttttcaat ctggcggcga agggcaccac cgaccctgta gcgactccgc
     5281 acccgatttt gtctgatctg cgcgttcggc aggcgattcg tgctgcgatc gatgtggata
     5341 cgatttcgaa agagtttttc ctcgggtatg ccaagcccgc ctggacggaa ttcttccgcc
     5401 cgccgtacac ttgcgatatt ccgcgcccga aatttgaccc cgctgccgca tctgctctgc
     5461 tggaggaagc aggctggaag gataccgatg gcgatggggt gcgtgaatgc cgcggctgtt
     5521 ccaccgctga agaaggctac aagatggaga tggaattcat cacctactct gaatatggcg
     5581 agcctttgga attgacccag cagtatattg ccgagcagct gggcaatatc ggaatcaaac
     5641 tcaacctgac cattgtggaa ggcagcgtct tgtgggcggc ttccaccgat ggcggtattg
     5701 aacaaagcgg taattttgac atggatattt gggatgacgg ttattcaggc gtggacccaa
     5761 ctgattttct ctggagttat tattccacgg atgcagccgt gcccgatgcg ggctataact
     5821 acagccgttg gtcaaatgaa cagtttgata ccctgttggg caatgtgtac acactggatg
     5881 aaggacaacg tcagaaggat ttctgtgaaa tggccaagct gctggatgct gaaattccgc
     5941 agttgttgct gttcacagct tcaaatgcag atgcgcacaa tgcccgtctg caaaatgtac
     6001 aatcctccac gaacgatctt gtgacgtgga attccgccga ttgggtgttg aaataacttg
     6061 gctacctaca ttttcaggcg tttattgcaa acggctggcc tgctggcttt gctgagtgtc
     6121 ctgctttttg cgctggtcaa tcttgcgccg gggggaccgc ttgcgggtca ggggcagagc
     6181 cggcacattg atcctgacaa ggctgaattg ctgaagcgtc aatttgggct ggataaaccc
     6241 ctgccgacgc aatatcttgt ctggcttgtc ggcaacgact ggatgcaggt ggacaaagat
     6301 ggcgatggtg ttttggacag ctacggcacg cggcagggaa ttttgcgcgg cgattttggc
     6361 ttttccttcc gcacccgtca gcctgtattg acggaaatcg gtcagcggct gccgaatacc
     6421 atctacttga tgacggtcac gatgggtgtg gcggtgttga tcgcgctgcc cattggaatc
     6481 atttctgcca ttcgtcagta ttccaccttt gatattacgg tgacaacctt ttcgtttgca
     6541 gggcaggcga ttcctgaatt ttggctgggc ttgatcctga ttctgatttt ttatgcctgg
     6601 ctcaaaaatc cattcacggg tgaaccattg ctgcctccgg gcggcatgac ctcatccaac
     6661 agcactggtt tcgaccttgg cgaccgtgtc attcacttga ttctgcctgt ggcgactggt
     6721 gcgctgggtt gggtggcatg gtattcgcgt ttccttcgtt caagcatgct ggaggtgatc
     6781 catcaggatt acatccgcac tgcgcgtgcc aaagggcttt cgaatctcaa ggtcttgtcc
     6841 aaacatgctt tgcgcaatgc gctgatcccg cttgtcactt tgctggcatt ggacctgcct
     6901 tatattttcg gcggcgcggt ctttgtggag atcctttttg cgtggcctgg tatgggcagg
     6961 ttgtattatc aggcggcgct cgaccgcgat taccccattt tgttggcggt gctgatcatt
     7021 ggcgcagggt tcatcattct ttcgaacctg ctggctgata tcgtctatgc ctaccttgat
     7081 ccccgcatcc gatttgagta gctcatgaac agaccagaat ccaatcatcc acttgtgagt
     7141 gagctacagg catccgccgc cacgtttcaa tcggccagca tgggcgcagg agtgtggaaa
     7201 cgcttccgcc gccacccggg tgcgatcttc ggttttgcca tcatattcgt tttggcagtg
     7261 ttaacgctgt tggcgccact ttccccctat gaccccgagg cttcggatct ttcagaacga
     7321 aacaacccgc catccctgag ccacccaatg ggcaccgatg cacttgggcg tgatttgctt
     7381 acccgtgtat tgttcggcgg gcgcatttcg ctgacggtag gcgcgttggt tgttgtcatc
     7441 tcacttttga tcggtgtgcc cattggcgcg ctggctggct attatggcgg cgttttggat
     7501 ggcattctga tgcgttttac cgattctttc ctttcactgc cttcttttct tgtgctcatt
     7561 ttgctgagtg caattctgcg tgaagtggat gctccgctct ttgaaaagaa caacgtcctg
     7621 acgatcagca tggtcattgg cgtttttgga tggacgacgt ttgcgcgact ggtgcgggcg
     7681 accttcctca cgcttcgaga aacggacttt atatctgctt cgcgtgccct gggtggatct
     7741 gaccttcgaa tcattgcgag gcacattctg ccgaatgcca tcggtccgat cattgtggag
     7801 gcaacccttg aattcggata cgccatcatc gaagagtcgg gattaagttt tctgggattc
     7861 ggtattcagc cgcccacgcc ttcatggggt aatttgttga gcaatgcgca ggagcatttt
     7921 acgaaatatc cctggctggc gatcttcccc gggttgatga tcttcctcag tattatttcc
     7981 ataaattaca tcggcgatgg cttgcgcgat gcctttgacc catacaaggt gcttgaaaaa
     8041 gttggcgaag tgtagtttta cggctttagc cgtttgaatc atcccattcg aataaaggag
     8101 agtattttat gtctggcaat cgtcctgcac ttgaaccgct tcgttccgca tttcgaattc
     8161 aatatcttac agaacagcaa ctggatacac ttcaagaagc cacacttgat attcttgaaa
     8221 acgttggcat aaaattccct tccgagcgcg ccttgaatgt gttcatcgaa cacggcgccg
     8281 atgtggataa aaatacccag gtggtcaaaa ttaaacgcga catggttttc aaagcgatga
     8341 aaaccgtgcc gcgttatttc gtcatgggtg cgcgtgtgcc cgaatatgac ctcaaccttc
     8401 aggaagggac gacctacttc accaccgatg gctgcggggt ggaggttgtg gatcttgaaa
     8461 cacgccaata ccgcccttca agcaaggctg atgtgggtat gatggcgcgg gttgccgatt
     8521 atcttccctc gatcgggttt tattggccta tggtcagcgc acaggattgc ggccgcactt
     8581 cccctttgca tgaactggag atcgggtgga ataacacggt caagcatttt cagtcagaga
     8641 ccattatggg tgggcgtgaa gccgcttatg ccgtggaaat ggcgactgtc attactggca
     8701 gcaaagagga gttacgcaaa cgtccgccgc tttcgtcttt gatctgtacg gtctctccgc
     8761 tgatgcagga ccagcccggc attgaggccg cgatggtttt tgccgaggcg gggattcctg
     8821 tcggcttttt ggccatgccc acgttaggga cgaccgctcc gatcacacag gcgggggcct
     8881 tggccatggc agatgcagag atcatcagcg ccacagtcct catgcagttg atgttccccg
     8941 gcgcgcctat ttatcattcg atgatgcagg cctgggctga tatgcgcacg ggtggttatg
     9001 tcagctatcc gcgtgatgca cgtggacgct atggcgtggt ggatatggcg catcattggg
     9061 ggatgccttc actgggcgca tgttacggca ccgattcggt tgttaccggc acatggcagg
     9121 ctgccgcaga gcctgcactc gatccatttt tggctgggct tgccagcccg gagatcgtca
     9181 ctggcatggg gctctcgcgc acttatacgc gtttattccc cgaacagatc attctggatg
     9241 atgatttgta tcaacgtgcc cgtgcctatc tccaacgcat ggaagttgat gaagactcgc
     9301 ttgcgatgga agcgatccga aatgtggggc cgggcgggca tttcctcggg caaaagcata
     9361 cccgcgccca tatgaagacc tcgcttgtgc gcggcgtgac ccatgaattg gatgctcaga
     9421 acaaataccg tgacccggtt gaagtggcgc gtgaacgtgt gcgatggatc atcgaaaatc
     9481 accaacccga accgctcgaa aaaagccagc aggaagaatt ggctcgtatt gtcgctgccg
     9541 cagataaaga gttagggtag ctttggcagg ttgcagttgg aaagcagatt atggaaacag
     9601 acttgaaggt tttgtcgagt aatgagatcg cgcagattca cgagcgtacg ctttccattc
     9661 tggcaaccac cggcgtgcgg gtggatacgg ccaagggtag gaaattcctc aaaaaagcgg
     9721 gcgcggaagt aaacgccact acgaatgtgg ttcgttttcc acgcgggatg gtcgaagaat
     9781 gtctgcaatt ggcgccgcgc agtttttcgt tgggcggcag gcgcccgaat tgggaatttc
     9841 cgttgaatgc aaacctttgt tcgctggttg ccgatggcgg tgcatctttt gcaattgacg
     9901 agcagaccgg cgagcggcgg gaggcgaatt ttcaagattg gctgggtgcc acccgcatga
     9961 tcgattcgtt ggatgaagtg ggggtgtact ggtcaatggt ggacccgggc ttttttcaaa
    10021 aatccgctgg cgattatgtg aaataccttc atcaaatggt ggggaatttc tcaaaacacc
    10081 tgcaggattg cacccacacc atcgaagaat cgcgctggat gttggaagtg ctgggaactg
    10141 tatttggcgg aaaagagaat gtacgtaaat tgcgtccctt ctcttatctg gtcacgccca
    10201 tctcgcccat ggtgctggaa gaacaacaca ccgatgccta ccttgaagtg atcgattggg
    10261 gcgtgcctgt ggcgatcatg cccatgccaa tgatgggtgg aacggcaccc gccagtttga
    10321 tttccacgat tttgcaggcg aactgcgaga cgctcgccat gctgtgtttg gtgcaggccg
    10381 catcaccggg gacgcccatc atctatgccg cctcgcctgc catcatggac ccatacacgg
    10441 gccgtttcac aggcagtgaa gtggaacatg gactgatggg agccgccatc actgaagtag
    10501 cacgttccta cggcctgccg gtggagacct cggctggaag cacagggcat tacgcgcccg
    10561 gtgtgcgtgc cagttatgaa cgcgccctca attgggtcat ggccacgttg tcctggcctg
    10621 atctgttggt ggggcctggt caactgggcg ggtcgttgtt gttgagcctg gaacagttat
    10681 taattgacgt ggaggtgttt catcgttctt cacggctttc atcaggaatt gatacgcggg
    10741 tcaaggaatg gctcgaagta gatctggccg ccgttgggca tggcggtaac ttcctgagtc
    10801 gacgttccac ccgcgaggca ttgcgaaatg gggaagtgta tatgaccgat tacgacgccc
    10861 gaaattccta cgaagcctgg gctgcctctg gtaaaccgac cattctggat gagttgaaac
    10921 ccaaggtgcg gaagattctc gacacacatt cacccctgcc gcttgatgaa gatgttgaac
    10981 gcgagcttga aagaattgaa cagcgtgccc gtgaaatgag aggataactt tatgcgtttt
    11041 caaagtcaga ttctaagtga tactgaaaaa gagcaggttc acaaggaggc attacaaatt
    11101 ctgcaaacta cgggcgcaaa gtttcatagc gacaaagcct tgaaacttct cgaagacaat
    11161 ggcgcacgtg tggatcgtga gaagcgcatt gcctacattc ctcctgaatt ggtggaacag
    11221 gcacttgcat ccactcccag gtcttttgtg cttggggcac gtaacccgaa atttgattac
    11281 cccatgccat cgcctgtgac ccgcttttgt ctggatggca cgggctcctt cgcacaggat
    11341 ttttacacag gcgaacaccg ctacggtacc ctcaaagata atgagaatgg attgcgcgtt
    11401 tttcaagcca tggatatggg cgtcatggcc tggcccccaa cctctgccga agacaagccc
    11461 gattattccc gtccgttaca tgaatgggct gcgatgatga agttttgttc aaaacatggt
    11521 gaacacgaat tgcacttcgt caatcaaacc ccgtattttg cagaaatgct tgttgccgtc
    11581 atgggcagtg aagatgaagt ccgcgcccgc catgcctact cattgaccta ttgccctgtt
    11641 tcaccgctca tacacgacgg tcccatgatg gatgcctata tcgaaatggg aagtttggat
    11701 gttcccatca tggtattgcc catgcccgca cctggtatga caggccctgc cagcttattt
    11761 ggcgcgatct gtctggccga tgctgaagcc ctttcttcac tcgtcaccta tcaactggcg
    11821 caccctggcc gcccgatgat attcagcagc gccaccggca ccatggatat gcggaacggt
    11881 gcgtttttgg gtggtactcc cgaaatggga ttgatgtctg ccgcgctgat cgaaatggga
    11941 cgtttctacg atcttccttc ctgttcagcg ggttgcacag ccgacgcacg cgagccaggg
    12001 gcagatgctg tgctcgaaaa agtcatcacc accatcccgc cagtgttggc agggtcagat
    12061 atcgtcctcg gcattggtgt tgttgaaagt gaccagcttc tcgttctcga gcagatcgtg
    12121 gtggataatg aaatcgcgca tttttgcgaa cgcatttttc aaggagtgga ttccgctccc
    12181 ggaagaatac tgacaaaaga tgttcaggcc gttggcccgg gtggaaattt tctctcccgc
    12241 aaatccacgc ttgaatttgc ccgcagcaag gaggttcact tttccgacct gttggaccgt
    12301 cacaccctcg accaatggtt gcagctaggc aagccatcca tgtattctgc tgcacgtaaa
    12361 aaagtggaag agattcttgc cgggccggtg cttgatccgc tttcggatga aacgataggc
    12421 acgttggatg agattctgat cagggcagac cgcgaacttg cctgatcttg atcgttggta
    12481 agttttgccc ggcaggcaaa aggtataatc tcctttgccc caatgcgaac ttcacctttc
    12541 cttcttcgtt cgtctcaata tgttctggct tttgttgtga tcgcctttgt gacggcggtc
    12601 ttgtttgtct tgcgcgaggc gttggacaca accctgattg cattgttgta cttaatcccg
    12661 ctggggttgg tcactgcttt tttggggttt ggcccaggga tcgccagcgc attgatgacc
    12721 tttcttgcat ttaactactt ttttatcaaa ccttattaca cctttgctgt ccatcgccca
    12781 acagatgtcg taattcttgt catctttctt gttgtagcaa ttgtgatcag ccagttggtg
    12841 gggcgtatgc aggtaagtct ggctgcagcg actgcccgcg aacgcgaagc cacccagctt
    12901 tacgaattga gcaccgctct ggcgggcttg catgatgaac aggtcattgt tcaaatatta
    12961 gccagacagg tacaggcagt attgctgggt gaggcggtta cggtccatat tgtgggtgga
    13021 caaccttttc agtttagcgt gcttgaaacc ggtcaaccgc agcgccctcc cgaatggttg
    13081 gtgcccattg aggcagcacg aggtgttctc ggggagatac gtgtttggag agcaggacca
    13141 gttcttacgt ccagtgagaa gcgtttattg cagacttttg ccagtcaggg ggccgtggca
    13201 cttgaacggg cgcggcttgt tcaggccgag tcacgtgcgc gagtccttga agagagtgac
    13261 catttgaaat ccattctttt gtcttctgtt tcgcatgaat tgcgaacccc tctttccacg
    13321 attaaagcgg cagcctcgag tctgcgcagc aatgaaatca attgggaatc gcctgcacga
    13381 ccggagttga tcgccgccat tgacgaagaa gccgaccatt taaatattct ggtcggaaac
    13441 ctgttggata tgtcgcggat cgagtcgggt gcgcttaaac ccaaacggga atggaatatt
    13501 ctggctgaga tcgttgaagg tgtgctggcg cgcatgaaac atcttttgga tgaacatatc
    13561 cttgaagtgg acgtgcccga gagccttccg cttgtacctg tagactatgt ccaaatcgag
    13621 caggtattta cgaatctgtt gagtaatgga gtgaaatacg cacctgcaaa aactttgata
    13681 cgggtgcgtg ctttcgtgga agatgatttt gttcacgtgg tggttggcaa tcagggtccg
    13741 caaattccaa gggagcacct tgaaaggatc tttgaaaaat tctttcgaat caccgccgcc
    13801 gaccgggtta caggtaccgg ccttggtctt tcaatttgca aaggaattat cgaggtacat
    13861 ggcgggcgcc tttgggctga gaatgttcct gatggattgg ccttcaactt taccctgccc
    13921 ctgatttggg aaggcgtgcc gccgcccaaa cttccgatgg atacggagac cgaatgagta
    13981 attcaccgca cattctcgta gtggatgacg aattgcagat ccagcgtgcc atccgcacga
    14041 tcttgaccga aaagggcttc aaggtttcca ctgcaagcag cggaaacgag ggtctcactt
    14101 tggctgcggc aaatgtgccg gatattgtga tccttgatct gggcctgccg gatatggatg
    14161 gtgctgaggt ttgtgtccgc ttgcgcgagt ggactcaatg cccgatcatc attctttctg
    14221 tacgcgatag cgaacgtgat aaagtttctg cgctcgataa aggtgcagat gactacctga
    14281 cgaaaccctt tggcatagaa gaactgctgg cgcgtgtacg tgtggccttg cggcatgcgg
    14341 cgagccagcc ggggcctcag agtaaggttg tcaaagccgg gcagttgacg attgatctgg
    14401 cctggcatgt tgtcaagctt ggtgaagatg aagtgaaatt gacaggcact gaatataaat
    14461 tgctggcgta tttggcgggt aaccgcggac gcgtgcttac gcatcagagc atcctttcac
    14521 atgtttgggg gcccgctgat gcagatcata ctgaatacct gcgcgtttat atgcggcagc
    14581 ttcgcaagaa actggaactc gatcccgaac gtccgcgtta cattctcact gagcctggta
    14641 ttggctatcg ctttattgcg gatgaataaa caggaccact ccacgcctta caacgccctt
    14701 gcaaaagggc gtttttattt gttctttata caattgcctg gaatttttgt catcgcttta
    14761 tatcagaggg ataggattca cttatctgtg aggtgaaatg aaaaaagtgg atttgactcc
    14821 tcccgttttt gcccttcccc ccgtttccgc cgttgacctt gggactgccc gtcgattaat
    14881 cgtgctggta cctgaaattg agatggatat tgcctatgtt gcacggaaac ttggagaatt
    14941 aacgaattcc ttcaacggct gtattttgtt ttacggtctt tgtaaggatg ctgtttgcga
    15001 gctgagcctg cgcaggcagc ttgtcgcttt aattgcgatg atggaagcgg aaaaactttc
    15061 catcgaatcg agaattgaga tcggagagaa ctggcccgcc tccgttttga ccgatcttcg
    15121 gcaggaagat atggttgtct gtttccccgc gccataccca ggctttgatc gcaggcccct
    15181 tcatcaattc ctttccgcaa atttgaattg cactgtttac atgcttgatg gtgttgcgca
    15241 tcgtgagacc cagtcatttg ctgagagggc ttccagcctg atggcttggg gcggctccat
    15301 tggtcttgtg tttgctttct tttggctgca ggcaaaaatt gttcaactgc cgcaagattg
    15361 ggttcatacg tccatgctgt atttttccct tttcgccgaa gtcggggcca ttttgggctg
    15421 gaatgcattg tttgattagt gcggtcgtaa aaacatgaat taactgaagt gatgacgata
    15481 tgattaatac aaacgaaaat aaaaactcac aaacttccat tattgaattg gatatagact
    15541 acaaacctcc acgcaccctg cgttcatggc ttattggccg tcccttatcc actgcggatg
    15601 catctcatca aaccattggg aaggtggttg ggctggctgt gtttgcttct gatgcacttt
    15661 cctccactgc gtatgccact caggaaatat tgggtgtaat tgcagcggct ggcacagtgg
    15721 catacggata tctttttccc atttcccttg ccattatcac cctcttggca attgtgacca
    15781 tttcttacga gcagacgatt catgcctacc ccggtggcgg tggcgcatac attgtagctc
    15841 gtgacaatct tggcgaattg cccgcccaaa ccgctggcgc agcacttctc actgattata
    15901 ttttgactgt gtctgtgtct atttcggcag gggttgcaca gattgtctct gcctacccgg
    15961 gtttatttcc gtatcgggtg gcaatcgcgg ttattgccgt actgcttatc atgttggtga
    16021 atttgagggg tgtgaaagag tctggtacgg cttttgccat tccaacttat ttttttgtgg
    16081 ttttaatgat cgccacggtg ggctatgggt tgttccgtta ctttaccggt tcgttgggta
    16141 tggtgattga tgctccgcat ttcgaagctc accatgtcat atcggtgatt acacctttca
    16201 ttcttctcca tgcatttgcc aatggcacta ctgccttgac cggtgtggaa gccatctcca
    16261 atggcataac cgccttcaag gaacctcgca gcaagaatgc aggcattacc ctgatctgga
    16321 tgtcgctgat tctgggttct ttgttcctcg ggctttcgtt ccttaccgga aaagtgcatg
    16381 ctgtgttttc ggaagaggaa accgtcattt cccaattggc gcggaccatc tatgacggca
    16441 gggggattcc gtatctgtta ttgatctccg gtacaactgt tattttgatc atggctgcga
    16501 atactgcatt tgcagatttt ccccgccttg gagcacttca tgcaggtgat gggttccttc
    16561 cacgccagct tacgtatcgc gggagtcggc tggtctattc tcgcggcatt gtgtcactgg
    16621 cagtgatcgc ttccgttttg atcatcattt ttcaggcaag tgtgactcgc ttgattccgc
    16681 tttatgcgat cggggttttc ctttccttca ccctttcaca ggcaggcatg gcgcgtcgtt
    16741 ggtggaagat cggtcacctc aaagaaggcg tggaaattgt tgagccaggt tcaacactga
    16801 gatatgaaag cgggtggcaa tacaggatgt tgatcaacgg ctttggtgct ctctgtacgg
    16861 cggtggtgat ggttgtgttt gcagtgacga aattcgcaga gggagcctgg gttgtactta
    16921 ttcttacacc gatccttgtg gcgatcttct ttgccattca tcatcactat aaaaggcttg
    16981 ccaaaaaact ctcgctggat aattttggcg ccattcctcc gcatgcgata cgacatcgtg
    17041 tcattttgcc ggtgagtggg gtacatcagg gcacactggc tgcgctgcgt tatgcgcgga
    17101 tgttgtctga tgatgttacg gctgtgcatg tcacaattga acctgcagag gctgaaaaaa
    17161 ttcgcaagaa gtgggagaca tggggaggag gggtccgcat tgtgatgttg gcatctcctt
    17221 atcgcctctt tttggaaccg ctgttgggct atatatcggc gatatcccgc cagcgtcagg
    17281 cgggtgagac catcaccatc gttgtcccgg agttcgtttc tgatgggcgt gtcacagctc
    17341 tgttgcacac aaataccgcg gatctacttc gcagccagtt gaaacggcag catggcattg
    17401 tgatcatcaa tgtgccgtat catgtgcatg aatgggacgg gtaagttgga ccaaaaagct
    17461 gtaagtactc gcagccgact ttcatacaga aagtcggctg tgtttttctg tcccaattag
    17521 gccttgacaa tatttactat acactgtata tttacactgt atagtaaata aggagccgac
    17581 atggtcagac ccagcaagaa aactcaaata cccaatttgc aggaagccat caaagaaacg
    17641 gcatggaagc agatcgcaga gacaggtgca gcagctttgt ccttgcgcgc gatcgctcgc
    17701 gaactcaaca tcactgcgcc tgccatctac aactactttc cagaccgcga cgcgctcgtc
    17761 accgcgctca tcattgatgc ctacacttct tttggagatt atcaaatcgc ctcacgcgac
    17821 tcccaggcag gggacgactt agcggggagt ttgcaagcta taggctttgc ctaccgaaag
    17881 tgggcgctgg aatatccgca acgttaccag ctgatttttg gtacgcccat tcctggctac
    17941 caagcgccga taatggaagt cttgccttcc gcagcacgtt ctttaagcgc tcttgtcagt
    18001 gtgattgaca aactgcggat agccaatgaa ttgaatgcag agggttttcc ttccattcaa
    18061 cctggttatg agcaaatgtt caacgtgtgg cgcggttttg caggggatta cgacatcttt
    18121 tcgctatcgg ttgccatgat tatttggagc cgcgttcatg gtctcgtctc attggagatc
    18181 tccaacaata tgccgccctt tggtgtggat ggcgcttcac tctatcgtta tgaaatggaa
    18241 tccatcgttc gtcaatttat aaaaggaaat tagaaatgta cactgtatcc attacccgct
    18301 tgcgagtccg ttcgatcttt ctcatgccgt tgtttacact tcatgccatg cgcaccagtt
    18361 cgcaggcaca aaaagcagaa ggcatccttg gcgtggaaac ccgctttgaa aaaaataacg
    18421 tcgtttggac caagaccatc tggacggaag aatccgcgat gaaaaagtat cgcggctcgg
    18481 gcgcacacca gatcgccatg cgtatccttt ctgagatgtg ctctgaagct tcggttacac
    18541 gttggcagca ggaaagcacg gaacttccca cgtgggaaga agcacatcgc cgtttgttgg
    18601 cggaagggaa gctttcaaag gtgaagcacc cttcaccgct gcaggcttct gggaagaccg
    18661 ctcctgaaac tatgcagtca ggcttcaaaa tgcctgctcc gccggtgacg caatgaattt
    18721 cgcaaaaagt tggatgttca atcaccccgt tacccgtcgt gtactggatg gatatttcga
    18781 gaacgatggt atccgccagc attacgtcac cttgggcgag ggcaagttgg ttgtgttgat
    18841 ccacggtttc cctgaatttt ggtattcgtg gcggcatcag atcgaagcgt tgtcacagga
    18901 ttatcaagtt gtggcaatcg accagcgcgg attcaacgag tcgggcaagc ctgc
//
LOCUS       DAOQLC010000083        18749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1029_length_18749_cov_20.065208, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000083 DAOQLC010000000
VERSION     DAOQLC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1029_length_18749_cov_20.065208"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..504
                     /locus_tag="PL000_14205"
     CDS             307..504
                     /locus_tag="PL000_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90104.1"
                     /translation="MRTPSAFGTSPKYDDSELGKFCRCFIVVFGGGRVGAALRELQSQ
                     SQEELDALLPSVLDRVFKGEL"
     gene            519..773
                     /locus_tag="PL000_14210"
     CDS             519..773
                     /locus_tag="PL000_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90105.1"
                     /translation="MAGKYAPLENYLRGLPATQKEVVLSFEQIERIINAKLPPSALEY
                     REWWANEKEGNHVNARAWANAGWKVESVDFNRKLARLVRA"
     gene            839..1195
                     /locus_tag="PL000_14215"
     CDS             839..1195
                     /locus_tag="PL000_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90106.1"
                     /translation="MAFRVRVVGVPVQDGSAVWVVDYVLWWPVDDYIGRNARPEFRES
                     VDSSLYLDHVGIFNKEEFIRLNDEYKASFLANPAKKQIHAEQQKRMDVVDQHLKKHLD
                     TKWILVEQYEWESGMN"
     gene            1217..1522
                     /locus_tag="PL000_14220"
     CDS             1217..1522
                     /locus_tag="PL000_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90107.1"
                     /translation="MNYWYSLNLGDGIMAGVPSAEIEAFFQRAFISAGKPPEMAVFTR
                     SESEGRLHCEVIAYFSPAAAEVALAFQAHACAQPARAGLGLLAGDEHSWSLLFPEGQ"
     gene            1552..2052
                     /locus_tag="PL000_14225"
     CDS             1552..2052
                     /locus_tag="PL000_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNC90108.1"
                     /translation="MRFGRTFYVAVLLLCLFEMARLWLITPDQMAAHFNAQGLPDRFA
                     PKAEFFWFQAQTLLVVILVSLPLQVLFLVLPPNLINMPNREFWLAPERREETIGRLNS
                     FGAMLFGVIALMIQTAFEISAYANLRTPIQFNAGLMGAIMVTGMVVIGLLLMQLLISF
                     RLPRTK"
     gene            2049..3395
                     /locus_tag="PL000_14230"
     CDS             2049..3395
                     /locus_tag="PL000_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90109.1"
                     /translation="MKSRPVYKAWGCWKVLFYLLFAACSPTPTTSPLENITVTATVPV
                     EVAVTPFETESIVSAVQIRQSEVELITDAAATGDGGNIWGGHQTRVVRTDAGVFTAYT
                     VSGKDELHRVWRLVRRNAVNDWVVVAEGDAGREPVNLLAAPDGTLYLVGWPEGVATLW
                     AGKPIDGSFAMEAQVIPNMIQGNWPYSSAGIASDGTLCIVASNGGEEPGGEFYWSCYL
                     PADRQWVSRISELDFRYCYTYVFPQADGQFALVSTRDVRWQALGYEQPADSFDYAFNA
                     IGFWRTDDLLNKPLELISFDEEPPTAAYPLPFLNAQMDAYLDTHGNMHILYWREGQTT
                     GGERWVMHRILSQMGEILFDERLPEDSGYFNRIFQDSHGRFYILGSSGLLYPLDENGE
                     HPTKPIRIDLGGHEVEYSGYGLSVPRTGSALVNTIDVVFPSGNETQWLYFQIEFSE"
     gene            3457..4104
                     /locus_tag="PL000_14235"
     CDS             3457..4104
                     /locus_tag="PL000_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90110.1"
                     /translation="METKQEYLLVVEDIPDILKLLEATLVFKGYRVVTASNGQEALEV
                     IQKERPAVVITDILMPKMDGFSLVHRLRINPETRDIPVVFLSATYVAPEDKTFALTIG
                     ATRFIEKPVALEEFLPVVEELLKKGAPTAATKLPNEFEFYDGYRKRLETKLNHKLTQI
                     TRDEHLVRTLSGAEKVSIETSLRHAIEERKEIELLLGQIRAQLEKLSKPERSADE"
     gene            4097..4291
                     /locus_tag="PL000_14240"
     CDS             4097..4291
                     /locus_tag="PL000_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90111.1"
                     /translation="MNKAEGRDYSAPKDESLQAFKDWILGIYFNLTGKKTDDMSEDQW
                     VAKWREFWQKQKDKQEKNNN"
     gene            4405..7011
                     /locus_tag="PL000_14245"
     CDS             4405..7011
                     /locus_tag="PL000_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90112.1"
                     /translation="MQTEISLESQLKDAAQLSEAEWAVLAERVGGTWLLRGVFHLNRS
                     AQTELAAIMARPSIDAWMCGALSGGHPRSSSIPEGSGLAGSRFFVFPVTGSSRAVLVG
                     ADRQSPTAQRIWKLTASLLSNHSSLPDQPFLPDLQSGLASDLPLAIEKVLNTIVQTVN
                     CQGAWLAIRRGESLDIQAEWNDSKAKNLSLSIDTNPILRRVNRSLSDLVVSRTHPNWD
                     AIPCGPLKPNTRIWICLPLVIGQRLIGVVALWRQTEFSSSELGQLRDLAQRVSPSVEV
                     LVTFSEMAAHLRRLGLLNDFVLTVSSAQSLDQVARRMFGLLARAFNTELIALFLRSSD
                     GRVLRDYRMVDGKLNVISVSLAGHSIQPILKEARLRRVTDAIKEGFVPVFKGARSALI
                     VPLKYRGQAIGVLIIENLHAEAYSQYDEHLMVVITSHMAGLIEYTRLREEAEGRARSL
                     GLIHEVVQQVIGLNDKKEVAHIAAELVAQYFKYELAAILLVDEEGSFSIQGIGGTQAA
                     VVQKALADQGNGEYIVREGVTGLVSQSGESILINDTRQEKLYKPLKGWEAHSEICVAL
                     KDGENILGIIDVESREQNAFSHNDLIAMESLAGILASVISSANQYQKLQETIRQLRAI
                     EIELNSRIEAQRSAENRLIQAAKLAAVGEMAAGIAHELNNPLTTVTGFSELVLEELPE
                     GSSHRDDLEMVLREARRASDVVHRLLDFSRQGERIRTRADLNEVVNDVIALTRHLIQT
                     NGVNLTLGLSSSLPWVSIDRNQMKQVLLNLIHNALQAMPKGGDLFVSTDSAVRAERDW
                     VIMAVKDSGIGILPANQDRIFEPFFTTKGDSGGTGLGLSVTYGIVTDHGGSIDIASEP
                     GKGSTFTVWLPI"
     gene            7014..8594
                     /locus_tag="PL000_14250"
     CDS             7014..8594
                     /locus_tag="PL000_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90113.1"
                     /translation="METPFVFVVDDEPGIAMLCKRLLSRSGYTVESETDPRKAINYLK
                     ENKIDLLLVDIRMPEVDGFEVIQHAQRLQPDAAMLIMTGHGTVETAIRALRQGVDGLL
                     LKPFSKGSDLVDAVKLALADSQQKRDAARTQALRPLFSVTESLLSETRRDPLLDLILS
                     AICGHLQCSHAACYQQNNETHEFTLVASRGREISKNHLNLISHIDESATPLLLNASGP
                     GNTTLRSLLTDLRLGAAILTPIQRPNLRMVLYAARNLNEPPFRESDLELFQILARQAA
                     VALENSRLYAEQLDYVRKVEDSQKALLQAEKMAAAGRLSASIAHEVNNPLQAVQNCLH
                     LAGREDLPAEKRQEYFNLARTELERLTVTVRRMLDFYRPGASSLETVDLVEMLQYILN
                     LMSKQLSEANVKVVVDFFGKISPIQAVGSQIQQVFINLILNAVDAMPEGGVLKIIARQ
                     SKGGVELLFQDQGKGIPEEKQSNIFEPFFSTKDGGTGLGLTVSYNIITAHGGVLELLP
                     ERGPGACFRIFLPIGGEQ"
     gene            8591..9322
                     /locus_tag="PL000_14255"
     CDS             8591..9322
                     /locus_tag="PL000_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90114.1"
                     /translation="MKSNILVVDDEPVARQSLADILKLEGHNVTAAPNGQAAVEYVRT
                     HPIDLMIVDLRMPGMDGLEVVQVVNQISPETEVILLTAFGSTETAIQALRLRIHDYLL
                     KPASPAQVLASVKKGLARRTAKSRTSNVTSADVDEAMSVFSLKDGTTVDLSRRQIKHK
                     NKIEHLTPAEGRLLRILIENEGKVFSHRELVLLVQGYDTSQREAPEILRPLVSRLRHK
                     LDQFPSLSERVVSVRGTGYLYEGNK"
     gene            complement(9466..10413)
                     /locus_tag="PL000_14260"
     CDS             complement(9466..10413)
                     /locus_tag="PL000_14260"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC90115.1"
                     /translation="MQKKHTAGVLAALSSAVFLGLAPVLGKLAITLGFSPFAVVALRT
                     GFAAGILLISITLFSPQYLYIFPVGLVGCMFAGAINGLGSLLYYLALQRLHANVGQLL
                     YSLYPFFVALWLILDHQAPSRLTLYRISLATAAVALLTGGPQQQIDIVGVFMMIGAAI
                     LYAMHLPINQRVLYEVPAPTVALYTLLSMSAVVVPVYLIFDRAWPIGEVPWLPVIGLT
                     FATVFSRIALFLGVKKIGGMQTALLGLAELLIAIIFSDIWLGENLSLLQWLGAAGLGL
                     SLILVMYEDTNPPTYSKRTGWLSWIRAPDLPKDIFGPYE"
     gene            complement(10442..12094)
                     /locus_tag="PL000_14265"
     CDS             complement(10442..12094)
                     /locus_tag="PL000_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC90116.1"
                     /translation="MSFIIKCLDCGHNAPYYPTSTNCPKCNSQWREAEYDYELIGKTL
                     PAKLAGRPFDLWRYRELLPVRNPNVSLSLGEGGSPLIRAVNLGMMLGCPNIYIKDERQ
                     GPTSSFKDRQASVTIAALKEAGITEMVAASTGNVAISYSAYASRAGMKLWAFVTSLVP
                     ATKMREIALYGSQVIKITGSYDQCKQVASEFARQRHLYQDMGARTITSIEAMKTIAFE
                     IAEQLTAIQGTDGAAPWRTPDWYVQAISGGMGPIGVYKGFREMKDMGWVNRIPAFAPI
                     QAEGCAPMVDSWKKGLEKAEPVVSPKTRIETLATGDPGRAYEFLRSYVNGTNGAFESV
                     SDEDAYRAMHVLAKMEGISAEPAAAVAFAGLFKLIRAGIIKPSDTVVVNCTGHTMPAE
                     PIILGENWSRDVKLPTSQETPQEGLLAALTQVAPERFPKIAIVEDTTEARRLIRRILQ
                     SQGNFTILEAGNGREGLELIQRELPDLIILDLMMPEMDGFTVIEALRAKQETATIPVI
                     VATAKELTPDEKNRLSGQIQSLMQKGDFLNDEFLEEVKTLIK"
     gene            complement(12109..>15551)
                     /locus_tag="PL000_14270"
     CDS             complement(12109..>15551)
                     /locus_tag="PL000_14270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90117.1"
                     /translation="AQLFNDAKQRNEELAVLNGLSGELSTLVGINNVAESVFKYTSKL
                     MRTSSFFLCLLDEKQMEVSFPLVIDDGEKVEVAPRKAGGNGLTDYVMRTNQTLFLPNR
                     VNERSLALGINITIIGNPTSAKSWLGVPLSLGNRILGAIVVQDPEEENVYTTHQRDVL
                     ITIAQQAAVAIENTRLFQETEKRADELSLINRVVSTLTGATNLNNTLGLVIRELVEAF
                     PVSRGAIALLNDEKQSFIVVAEHSKQDNHNSALGLEIPIKGNTSTESIISTRKPYILE
                     DAANNPANPPEIREEFQRLGIVYSAIFPIIVNDTVIGSLGVDTTQKDTRLDPNEITLI
                     ETTLSPISATISRLNAEEALSRSEADLRTLFSSMEDVVLVFDADGRYIRIAPTNPGRL
                     VRPPEDLLGKLMHEVLPANVADQSVAAIRHTLETGETNQLEYELQIGEEKFWFLANLT
                     KLKDDQVFWVARDITDRKKAEESIRRRNAYLAASSEIGKIVTSTLDLNTIFARTVNLI
                     NERFGFFHASIFIVEETGFNALLRESTGAAGAEMKKNKHSLPVNDKSIVGKVALDGQA
                     IVVNDVKIDSLHKFNPLLPETAAEAAIPLRIGNRVIGVIDVQSKSTNSFTDDEISVLQ
                     TVADQVAVAIDNARSFELSQQAVMEMREIDRLKSQFLANMSHELRTPLNSIIGFSRVI
                     IKGIDGPVTELQQQDLTAIYNSGQHLLGLINDILDLSKIEAGKMELAFDEVNIVDITS
                     SVLSTMSGLIKDKPIQMKRILEPNLPTVRADAIRIRQVMINLLSNAAKFTDEGDILVE
                     VGLKPGQGSTRRELRVSVTDTGPGISVQDQAKLFQPFSQVDDSPTRKTGGTGLGLSIC
                     QHIVNMHGGRIWIESEVGKGSTFHFTLPLYRKEEEDADSGRKTVLTIDDDPQVIGLYE
                     RYLQQQGYQVVPLSDPSMAVERAKQVKPFAITLDVMMPGIDGWQVLDKLKADPETRNI
                     PVIVCSIIEELEKGFNLGATDYLVKPILEDDLVNALDRLNSDGSIREVLVIDDDPDDL
                     RLIGKILTDDGRYKASLAEGGRMGWDIISSGNPPHAVILDLFMEEMDGFRILENMQAS
                     EKLRNIPVIIVSGMDLSAEQKEKLNESGQRLLTKGTFSEKELLISIQRSLEKAQAKNK
                     "
     assembly_gap    15552..15651
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15652..16637)
                     /locus_tag="PL000_14275"
     CDS             complement(<15652..16637)
                     /locus_tag="PL000_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90118.1"
                     /translation="MPKKPKIDKRLNKLFKDINLEEASSSLKQAAKDEAESKASVTET
                     APASKPDPKPASKKTDTRPIPRQGRIHTSTLIPPEPVITEEHENIASTYAVNIQTGYQ
                     DWATLRILDETTQRQWSQDEELLVKQVTDQLSLALENAQLFQETQKRANELATLNEIV
                     QAISEQIELKNVLNTAYEQIVKLIPLDAFFVALYDESSNTVSYPIVIDEGKSYEEPNE
                     ALNPENFTGRVIKSGKLLLEAVKPEDLKSKSINSDSFGNKNKNYGTMLFLPLRSVNKT
                     MGCLSIQNYDYFAYKQEDIALLENIANQISIGIQNAQLFNDAKQRNEELAVL"
     gene            complement(16603..17391)
                     /locus_tag="PL000_14280"
     CDS             complement(16603..17391)
                     /locus_tag="PL000_14280"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC90119.1"
                     /translation="MIKKIAISNEKGGVAKTTSTISLGAALVDMGNRVLLVDLDAQAN
                     LTLALGLEPGESAITSSEVILDNIPLLNALRKTEIENLDLIPSNSRIETAEQFLPVRT
                     SYTTTLQRAIASAPNLHYDYILFDCPPALGAITQNALIAADLLIIPTQAEYFSAYALR
                     NMMTLIRRIREGENPNLAYRVLVTMLDKRNRTHRNIHDQLRSTFGEGVFSTVIETDTK
                     LRESPIAGLPISHYRPGARGSIQYRVLAQELIEYAKETENRQTA"
     gene            complement(17407..>18749)
                     /locus_tag="PL000_14285"
     CDS             complement(17407..>18749)
                     /locus_tag="PL000_14285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90120.1"
                     /translation="KATSGQTRNTVLISIAIVFIAAVSAVAVSNVFTNPLQQLTSVAE
                     KISAGDFSQQAEIKTHDEIGLLAGAINEMASQIRGFIASLENRVEQRTLELAQRTNEL
                     EKLTEQSKKRADELQIIAEISGYISAEKDLESLLPLITKTVSERFGFYHVGIFLLNEN
                     GKFAVLRAANSPGGQVMLKRQHKLEVGQVGIVGNVTATGIARIALDTGADSTFFNNPD
                     LPETHSEMALPLIARGTIIGALDVQSTFPNAFTQNDISILGLLADQIAIAIDNVRLLD
                     ETKKALAEAQSSFREYLAGAWQNKSASEIFGYYQTLTGGQLITGEKTPEVVAKTEKDE
                     NALSIPIQLRDQVIGTVHIRPNKEDRAWSRDEINIVNAVAERLGLALDNARLFEETSS
                     RASRERLVTEITTKIRGTNDPQEMIRTAVQELQRALGASRVEIVPRKNAPPPDK"
BASE COUNT         4416 a         4357 c         4902 g         4974 t
ORIGIN      
        1 tgatgatatt gaattttcac accaagcggc tagtgttact aaaagagatg cagtcgcaaa
       61 cgcaagagga attggatgca ttgttaccgt ccgtgctgga tagggcgttt aagggagaat
      121 tatgatgtca ttgcgaggag cgttcgctgc gacgtggcaa tctcccgtta caagagggga
      181 ttgcttcgca acgtgcgctc gcaatgacat gcaagaaaat gcgttgaggg ggttgcagtc
      241 gcaaagtcaa gaagaattgg atgcattgct gccgtccgtg ctggataagg cgtttaaggg
      301 agagttgtga gaaccccctc cgccttcggc acctccccca aatacgacga ttcagagttg
      361 ggaaaattct gtaggtgttt cattgtcgta tttggaggag gacgggtggg ggcggcgttg
      421 agggagttgc agtcacaaag tcaagaagaa ttggatgcat tgctgccgtc cgtgctggat
      481 agggtgttca aaggtgaatt gtagagaaag gaaaaattat ggctggtaaa tatgctccgc
      541 tcgaaaatta cttgcgtggt ctacctgcaa ctcaaaagga agttgttctt tcatttgaac
      601 agattgagag aataataaac gccaagctgc caccatctgc ccttgagtac agagaatggt
      661 gggcaaatga gaaagaagga aaccatgtca atgcacgagc atgggcaaat gctggatgga
      721 aagttgaaag cgttgatttc aacaggaaac tagcaaggct ggttcgagca tgatagcaaa
      781 ggtcaacgct gtgagggagc tgcagtcgca aattcaagga gaaaaggaga attgtgaaat
      841 ggcgtttaga gtcagagtcg taggggtgcc agttcaggat ggcagtgcag tttgggtggt
      901 ggattatgtt ttgtggtggc ctgtggacga ttacattggt aggaacgccc gccctgagtt
      961 tcgggaaagc gttgatagct cactctacct tgatcatgta ggcattttca ataaggagga
     1021 gttcatccgc ttgaatgatg aatacaaggc cagttttctc gcgaaccctg cgaaaaagca
     1081 aatccatgct gagcagcaaa aacgtatgga tgttgttgac cagcacctaa aaaaacatct
     1141 ggataccaaa tggatcctgg tggagcaata tgagtgggaa agcggtatga actgacggct
     1201 ggcagatggg agagttatga attactggta ttcgctcaat cttggtgatg ggatcatggc
     1261 tggtgtgccc tctgcagaaa tcgaggcgtt ttttcaacgt gcatttatct ccgcagggaa
     1321 gccgcctgaa atggctgttt ttacccgttc tgagtcagag ggtcgtttgc attgtgaggt
     1381 gatcgcttat ttttcacctg ccgctgccga agtggctttg gcatttcagg cacacgcttg
     1441 tgcccagcct gcccgggcag gtttggggtt gttggctggt gatgaacatt catggtcgct
     1501 tctgtttcct gaaggccagt aatcttcgcg aggacacgag gagcttgtga tatgcgtttc
     1561 ggaagaacct tttatgttgc cgtattgttg ctctgccttt ttgaaatggc tcgcctttgg
     1621 ctcattaccc cagatcaaat ggctgcgcat tttaatgctc agggtctccc agatcgtttt
     1681 gctccaaagg ccgagttttt ttggtttcag gctcaaaccc tgctggttgt tattttagtc
     1741 agcctgccgc tgcaggtttt attcctggtt ctgccgccca acctgatcaa catgcccaac
     1801 cgtgaattct ggcttgctcc tgaacggcgg gaggaaacca ttggccgcct aaattcattt
     1861 ggagccatgc tgtttggcgt gatcgcgctg atgattcaaa ccgcattcga gatttcggct
     1921 tatgcaaatt tgcgaacacc gatccaattc aatgcggggt tgatgggtgc gatcatggtc
     1981 actgggatgg tagtgatcgg cttgttgttg atgcaattgt tgatctcatt tcgtctgccc
     2041 cgcacaaaat gaaaagcaga cctgtttaca aggcgtgggg ctgttggaag gttttgtttt
     2101 atcttttgtt tgccgcctgt tctcccaccc caaccacttc gccgcttgag aatatcacgg
     2161 tcaccgcaac tgtacctgtt gaggtggctg tcacgccttt cgaaacagaa tccattgtca
     2221 gcgcggtaca gattcgtcaa agtgaggtgg agttaattac cgacgccgct gctactggtg
     2281 acggaggtaa tatttggggt ggacatcaaa cccgtgtagt ccgcaccgat gcgggggtgt
     2341 tcactgctta tacggtttct ggaaaagatg aattgcatcg tgtctggcgt ttggtcaggc
     2401 ggaatgctgt aaatgactgg gtcgtagttg cggagggaga tgctgggcgg gagcctgtca
     2461 atctgcttgc ggctcccgat gggaccttgt atctggtcgg ctggcctgag ggtgtagcca
     2521 cactttgggc agggaaaccc attgacggat cttttgcaat ggaggcgcag gttattccga
     2581 acatgataca ggggaattgg ccgtacagtt ctgcgggcat tgcctctgat ggtacgttgt
     2641 gcattgtagc aagcaatggc ggagaagaac ctggcgggga attttattgg tcttgttact
     2701 tgcctgctga tcggcaatgg gtcagccgca tcagtgaatt ggattttcga tattgttaca
     2761 cttatgtttt tccgcaggca gatgggcagt ttgctctggt ttccacaagg gatgtccgct
     2821 ggcaggcttt ggggtatgaa cagcctgcgg attcttttga ttacgccttc aatgcgatcg
     2881 gattttggcg cacggatgat ctgctcaaca aacccctgga attgatctct tttgatgaag
     2941 agcctccaac ggcagcgtat ccactgcctt ttttgaacgc acaaatggat gcttatctgg
     3001 atacgcatgg caatatgcat atcctttatt ggcgggaggg gcaaaccaca ggcggagaac
     3061 gctgggtgat gcatcgcatt ctttctcaaa tgggagagat actttttgat gaacgcctcc
     3121 ccgaagattc tgggtacttc aaccgcattt ttcaagattc tcatgggcgt ttttatattt
     3181 tgggttcctc aggtttgttg tatccactgg acgaaaatgg agaacacccc acgaagccga
     3241 tacgcattga ccttggcggg catgaagttg aatattctgg gtacggtctc tctgttccac
     3301 gcactggctc tgctttggtg aataccattg acgtggtgtt cccttcaggg aatgaaaccc
     3361 aatggttgta ttttcaaatt gagtttagcg agtgacgggt atgctttaca tgatattgac
     3421 atgtccagac accccgcaat ggattagaat tgagttatgg aaacaaaaca agaatatctg
     3481 cttgtagtag aagacatccc cgatatcctc aaattattgg aagcgaccct cgttttcaag
     3541 ggatatcgcg tcgtcaccgc cagcaacggg caggaagctc ttgaagtcat acagaaggaa
     3601 cgtcccgccg ttgtcattac cgatattctg atgcccaaaa tggatggttt cagccttgtg
     3661 catcgcttgc gcatcaaccc tgaaacccgt gatattcccg tggtctttct ttcggccacc
     3721 tatgtggcac ctgaagacaa aacctttgcg ctgaccatcg gtgccactcg gtttatcgaa
     3781 aagcccgttg ctttggagga attcctccct gttgtggagg aacttttgaa aaaaggcgcc
     3841 ccaactgccg cgacaaaact ccccaatgaa tttgagtttt acgatggcta tcgcaaacgc
     3901 cttgagacaa aactaaacca caaactcacc cagatcaccc gcgacgaaca cctggtgcgg
     3961 accttgagcg gggcggagaa ggtatccatc gaaacctccc tgcgccacgc catcgaagaa
     4021 cgcaaagaga tcgagctttt gcttggtcag atccgcgcac agttggaaaa actttccaag
     4081 cctgaaaggt cggcagatga ataaggcgga aggccgcgat tattccgctc cgaaagacga
     4141 atctttgcaa gcattcaaag attggatcct gggcatatac tttaatctga cgggcaaaaa
     4201 aacggatgac atgtctgaag atcaatgggt ggctaagtgg cgggagtttt ggcagaagca
     4261 aaaagacaaa caggagaaaa ataacaatta gaatgcatcc cgcccagatt ccgagtgttg
     4321 gaatttgggc gggcggagcc ttgttcttga acagccaatt ttggcaatag acgcaggggg
     4381 gagtgtataa tttggcatac cgccatgcag actgaaatca gcctcgagtc tcaactcaaa
     4441 gatgccgcac aactttccga agccgagtgg gctgttcttg cagagcgcgt tggcggcaca
     4501 tggctcttgc gcggagtttt ccacttaaac cgttcggccc aaaccgagtt ggcagccatc
     4561 atggcaaggc cgtccattga tgcctggatg tgcggcgcct tgagtggggg gcatccccgt
     4621 tcaagctcca ttcctgaagg gagcggactc gctgggagtc gcttcttcgt ttttcctgtt
     4681 acggggtcat cacgggctgt tttggttggg gcggatagac aatctcccac cgcccagcgg
     4741 atctggaaac tgaccgcctc gctcctctcg aatcattcct ccctgccaga tcaaccgttt
     4801 ctacctgacc tgcaatctgg tttggcctcc gatctgccgc ttgccatcga aaaagtgttg
     4861 aacaccatcg tgcagaccgt gaattgtcag ggcgcgtggc tggcgatccg acgcggcgaa
     4921 tcgctcgata ttcaagccga gtggaacgat tcgaaagcca aaaacctttc cctgtccatt
     4981 gatacaaatc ccattctgcg ccgcgtcaat cgttctcttt ctgacctggt ggtttcgcgt
     5041 acacacccaa attgggatgc gattccatgc ggcccgctaa aaccaaacac acggatatgg
     5101 atctgcctgc ccctggtcat tggtcaacgc ctgatcgggg tggtggcgct ctggcgtcag
     5161 accgaatttt caagcagtga gttggggcaa ttgcgcgatc ttgcccagcg cgtctctccg
     5221 tccgtggaag tgctggtcac cttttcagaa atggcagccc atctgcgcag gctgggcttg
     5281 ctcaatgatt ttgtcctcac ggtttcctcc gctcaaagcc tcgatcaggt tgcccgccgc
     5341 atgtttggtc tgctggctcg tgccttcaac accgagttga tcgccctctt cctgcgctcc
     5401 agtgacggcc gtgtgttgcg cgattaccgc atggttgacg gcaaattaaa tgtcatcagc
     5461 gtttccttgg ctgggcattc cattcaaccc atactcaaag aagcacgttt acgccgtgtc
     5521 acagacgcca tcaaagaggg atttgtcccc gttttcaaag gcgcacgctc ggcgttgatc
     5581 gtccccctca aataccgcgg acaagcgatc ggcgtcctta ttattgaaaa cctgcacgca
     5641 gaagcctaca gtcagtacga tgaacatttg atggtggtta tcaccagcca tatggcaggg
     5701 ttgatcgaat acacccgcct gcgcgaagag gccgaaggcc gtgcccgcag cctggggctg
     5761 atccacgaag tggtacagca agtcattggc ttgaacgata aaaaagaagt ggcgcacatt
     5821 gccgctgaat tggttgcgca atatttcaaa tatgaattgg ccgccattct gctggtggat
     5881 gaagagggaa gtttttcgat tcaaggcatc ggtggtacac aggcggcagt ggttcagaaa
     5941 gcgcttgctg atcagggcaa cggcgaatat atcgtccgtg aaggggtgac ggggcttgtt
     6001 tctcagagcg gcgagagtat tttgatcaac gatacaaggc aggaaaagct ctacaagcct
     6061 ctcaaaggct gggaggcaca ctctgaaatt tgtgtggcct tgaaagatgg tgaaaatatt
     6121 ctcggcatca ttgatgttga aagccgtgaa cagaatgcct tctcgcacaa tgacctgatc
     6181 gccatggagt cactggcggg tattttggct tccgtcattt ccagcgcaaa ccaatatcaa
     6241 aagctgcaag agaccatccg tcagttaagg gcgatcgaga tcgagttgaa ttcccgaatt
     6301 gaagcacagc gttccgctga aaaccgcctc attcaggcag cgaaacttgc cgcagtgggt
     6361 gaaatggccg caggcattgc ccacgagttg aacaatccgc ttaccacggt gacaggcttc
     6421 tccgaacttg ttttggagga actgcctgaa ggttcgtcgc atcgtgatga tttggaaatg
     6481 gttctgcgtg aagcacgccg agccagcgac gttgtccatc gtctgctgga tttttcgcgg
     6541 cagggagagc gcatccgcac ccgtgccgat ttgaacgaag tggtcaatga tgtcatagca
     6601 ctcaccaggc atctcattca aaccaacggg gtaaatctca cattgggttt gagcagcagc
     6661 ctgccctggg tttccattga ccgcaatcaa atgaaacagg ttttactcaa cctgatccac
     6721 aacgctttac aagccatgcc caaaggcggc gatttgtttg tatcaactga ttcagccgtg
     6781 cgtgcagaac gcgattgggt tatcatggcg gtcaaagaca gcgggattgg cattcttccc
     6841 gcaaatcaag atcgcatctt cgagccattc ttcaccacaa aaggagatag cggcggcacg
     6901 ggcctggggc tttctgtaac ttacggcatt gtcacagatc acggtggaag tattgacatt
     6961 gcaagcgagc ctggaaaagg ctctacattc acagtttggc tgccgatcta aacatggaaa
     7021 ctcccttcgt ctttgtcgtt gatgatgaac ctggcattgc aatgctttgc aaacgcctgc
     7081 tctcgcgttc ggggtacacg gtggaatcag agaccgaccc gcgcaaagcc ataaactacc
     7141 tcaaggaaaa taagatcgac ctgttgttgg ttgacatccg catgcctgaa gtggatggct
     7201 ttgaagtcat tcaacatgcg cagcggcttc aacccgatgc ggccatgctc atcatgaccg
     7261 ggcatggtac agtggagaca gccattcgtg ctttgcgtca gggagtggat gggcttttgc
     7321 ttaaaccgtt cagcaaaggc agcgaccttg ttgacgcggt caaactagcc cttgccgatt
     7381 cgcagcaaaa gcgcgatgcc gcccgcacac aggcattgcg cccgctcttc tcggttacag
     7441 aatccctgct ttcagaaacg cgccgtgacc ctctgcttga tctcatcctc agcgccattt
     7501 gcgggcacct gcaatgttct catgctgcct gctaccagca aaacaacgaa acgcatgagt
     7561 ttacactcgt ggcttcccgc ggccgtgaaa tttcgaagaa ccatcttaac ttgatttccc
     7621 acatcgacga atctgccacg cctttgcttc tcaatgccag tgggcctggg aacaccaccc
     7681 tgagatcgct tctcaccgac ctgaggcttg gtgcagcgat cctgacgccg attcagcgcc
     7741 caaacctgcg catggttttg tatgccgcac gcaacctgaa cgaaccgccc tttcgcgaat
     7801 cagatctgga gttgttccaa attcttgccc gtcaggcggc tgtcgcactg gaaaattctc
     7861 gtctgtatgc cgagcaattg gattacgtcc gcaaagtgga agattcacaa aaggccctgc
     7921 tgcaggcaga gaagatggcg gcagcgggtc gtttgagtgc atccattgcg catgaggtca
     7981 acaacccgtt gcaggctgtg cagaactgtc tgcaccttgc aggtcgagaa gacctgcccg
     8041 ccgaaaaacg ccaggaatac ttcaaccttg cccgcaccga gcttgagcgt ttaacggtca
     8101 ccgtgcggcg catgcttgat ttttaccgtc cgggcgcctc ctcgcttgaa acagttgacc
     8161 tggttgaaat gcttcaatat attttgaatt tgatgtcaaa acaactgtct gaagcgaatg
     8221 tgaaggttgt tgttgatttc tttgggaaga tttcacccat acaagcggtc ggcagccaga
     8281 tccagcaggt gtttattaat ttgatcttga atgccgtaga tgccatgccc gaaggcgggg
     8341 tgctcaagat cattgctcgt caatccaaag gaggtgtcga attattgttc caggaccagg
     8401 gcaaaggtat ccctgaagaa aagcaatcca atatttttga gccgttcttc agcacaaaag
     8461 atggcgggac agggttgggt ttaactgtga gttacaatat aatcaccgcg catggcggcg
     8521 tactggaatt gctcccagaa cgcggccccg gtgcatgttt tcgaatattc ttacccatag
     8581 gaggagaaca atgaagtcca acattctagt ggtcgatgat gagcccgttg cccgtcagtc
     8641 tctggcagat attttgaagc tcgaaggcca caacgttaca gccgctccga acgggcaggc
     8701 cgcggtggag tatgtgcgca cacatcccat cgacctgatg atcgtagacc tgcgcatgcc
     8761 tggcatggat ggtttggaag tggtgcaggt ggtgaatcag atttcacctg aaaccgaggt
     8821 gattttgctc acagcctttg gctccactga aacggccatt caagccctgc gattgcgcat
     8881 ccacgattat ttgttgaagc ctgcctctcc tgcacaggta ttggcaagtg taaagaaagg
     8941 tcttgcccgt cgaactgcaa agtcacgtac cagcaatgtg acttctgctg atgtagacga
     9001 ggcaatgtcg gtcttttccc ttaaagatgg taccactgtg gatctttccc gcagacagat
     9061 caaacacaag aacaagatcg aacatctcac acccgctgaa ggcaggttgt tgcgaatctt
     9121 gattgaaaac gaaggcaaag tgttttcaca tcgtgaactg gttttactgg tacaaggtta
     9181 cgacacctct cagcgcgagg ctccggagat tttacgtccg ctggttagcc gcctgcgcca
     9241 taagctggat caatttccct cactctctga acgggtggtg agtgtgcgag ggacgggata
     9301 cttgtacgaa ggtaataaat aggcgtaaaa agtaattacc cccgaaattc caattttgga
     9361 ttttgacact tgcattcgtg caagcgagat gaagacgctg attggctcta acaagggcaa
     9421 aatcagcgtc ttctatattc tactcggtca cctgtttgtt gattttcatt cgtagggacc
     9481 aaaaatatct tttggcaggt ctggcgcacg aatccacgag agccagcctg ttcttttgct
     9541 atatgttgga gggtttgtat cttcatacat aacgaggatc aggctcaggc caagtccagc
     9601 tgcgccaagc cactgaagga gagataggtt ttcgccaagc catatgtcgc tgaagatgat
     9661 ggctatcaat aattcggcaa gtcccagcag ggcggtttgc attccaccga ttttcttcac
     9721 ccctaaaaat aaggcaatac gcgaaaaaac agttgcaaat gtcagcccga tgactggtaa
     9781 ccagggtact tccccaatgg gccatgcccg gtcaaagatc aagtaaacgg gaacaaccac
     9841 agcgctcatc gaaagaagag tgtataacgc cacagtgggt gctggtactt cataaagcac
     9901 gcgctgattg atcggcaaat gcatggcata caagatggca gcgccgatca tcataaaaac
     9961 gcccacgatg tctatttgtt gttgtggccc gccggtgagc aatgccacgg ctgccgtagc
    10021 aaggctgatg cgataaagtg ttaatcggct tggtgcctgg tggtcaagga tcagccaaag
    10081 ggcaacgaaa aatggataaa gcgagtaaag caattgccca acattggcgt gcagccgttg
    10141 cagggcaaga taataaagca gggagccaag accgttgatg gctccagcaa acatacatcc
    10201 aaccaacccc acagggaaga tgtataaata ttgaggagag aatagggtga tggatattaa
    10261 aagaatacca gcggcaaacc cagtccgaag tgccaccact gcaaacggcg agaaacccaa
    10321 tgtgatcgcc agttttccca gaacaggtgc taacccgaga aatacagctg aacttaaggc
    10381 ggcaagaact ccggccgtat gttttttctg caaaggtacc agcctatggt cattttgaaa
    10441 gctatttgat gagcgttttt acttcttcca ggaattcgtc gttcaggaaa tcgccttttt
    10501 gcataaggga ttgaatttgt ccactaagtc tgttcttttc atcgggggta agttctttgg
    10561 cagtagccac aatcaccggg atggtggcgg tttcctgttt ggcacgtaag gcttcgatta
    10621 cagtgaatcc gtccatttca ggcatcatca gatcaagaat gatcaggtca ggcagttctc
    10681 gttggatcaa ttcgagtcct tcgcgaccat tgcctgcttc cagaatggtg aaattaccct
    10741 gcgattgcaa aatgcgccga ataaggcggc gtgcttcagt agtatcttca acaatggcaa
    10801 tcttggggaa acgctcaggc gccacctgtg taagtgctgc cagtagacct tcctgcggtg
    10861 tttcctgact ggttggtaat ttgacatctc ttgaccagtt ttcaccgaga atgatcggtt
    10921 cagcgggcat ggtgtgccct gtgcagttga ccaccactgt gtcactcggt ttgatgatgc
    10981 ccgcacggat caatttgaaa agacctgcaa acgcaacagc tgcagcgggc tcagctgaaa
    11041 tgccttccat ttttgccaat acgtgcatgg cacgatacgc atcttcatca ctgacacttt
    11101 cgaacgcacc gttggtgccg ttgacgtagg aacgtaaaaa ttcgtaggca cggcctgggt
    11161 caccggtcgc caaagtttca atgcgggttt taggcgatac gacgggttcg gctttttcca
    11221 agcccttttt ccagctatcc accatggggg cacagccctc cgcttgaatg ggggcaaatg
    11281 caggaattcg attgacccat cccatgtctt tcatttcgcg gaaaccctta tagacaccaa
    11341 ttggccccat gccgccgcta atggcctgca cataccaatc aggtgtgcgc catggggcgg
    11401 ccccatccgt cccctggatc gctgttaatt gttctgcaat ttcgaaagca atggttttca
    11461 tcgcttcaat ggaggtaata gtgcgcgcgc ccatgtcctg atacaaatgg cgctgacggg
    11521 caaattcaga agcgacctgt ttgcattgat cgtatgagcc cgtgatcttg ataacctgac
    11581 ttccatacaa cgcaatctca cgcatttttg tggctggtac aagactggtg acaaatgccc
    11641 ataatttcat ccctgctcgg gatgcgtatg ccgaatagga aattgccaca ttcccggtgg
    11701 atgcagccac catttctgta attcccgcct ctttaagcgc agcaatcgta actgaagcct
    11761 gcctatcctt gaaagaagat gttggacctt gacgctcatc cttaatgtaa atattggggc
    11821 aaccaagcat catccccaga tttacggcgc gtatcaatgg tgagccgcct tctccaagag
    11881 aaagactcac attgggatta cgtacaggca ataattcgcg atagcgccag aggtcaaaag
    11941 gacgccctgc cagcttggct ggcagggttt tcccaataag ctcataatcg tattctgctt
    12001 cacgccactg gctgttgcac ttcgggcagt tcgtagacgt gggataatac ggtgcgttat
    12061 gtccacaatc aaggcacttt atgataaatg acacgatatt gaaaactcct atttattttt
    12121 cgcctgggct ttctccaatg aacgttggat cgaaatcaat aattcttttt cactgaaggt
    12181 gccctttgtc agcaagcgct gaccggattc atttaatttt tctttttgct cggcggaaag
    12241 atccattcca ctgacaatga tgactggaat gttacggagc ttttcacttg cctgcatgtt
    12301 ttccagaatt ctaaatccat ccatctcttc catgaacaga tcgagaatca cagcatgtgg
    12361 aggattgccc gaggaaatga tatcccatcc catgcggccg ccttcagcga gtgaggcttt
    12421 gtaacggcca tcatcagtta ggatttttcc gatcaggcgc aggtcgtccg ggtcgtcgtc
    12481 aattacaagg acttcacgaa ttgagccgtc agagttaaga cggtcaagcg cattgacaag
    12541 gtcatcttca aggatgggtt tgaccagata atcggtcgcg ccgaggttga agcccttctc
    12601 caattcctca atgatactac agacaatgac cggaatgttg cgcgtttccg ggtcggcttt
    12661 taatttatcc aaaacctgcc agccatcgat ccctggcatc atcacatcga gtgtgatggc
    12721 aaatggtttc acctgttttg cgcgttccac agccatggat gggtcggaaa gcggtacaac
    12781 ttgataccct tgttgctgta aatatctttc atacagtccg atcacttgag ggtcatcatc
    12841 aatggtcagg acagtcttcc ttccactgtc tgcatcctct tcttcctttc ggtaaagcgg
    12901 aagtgtgaaa tggaaagtgc tgcctttccc aacttcactt tcgatccaaa ttcgtccgcc
    12961 gtgcatgttg acaatatgtt ggcagatgga caggcccaaa cctgtaccgc cagttttacg
    13021 agttggtgaa tcgtccacct gagagaatgg ctggaataat tttgcctgat cctgaacgga
    13081 aataccaggg cctgtatcgg ttacgctgac tcttaattcg cggcgggtgg atccctgccc
    13141 aggtttgaga cccacttcaa caagaatatc accttcatcg gtgaatttag ccgcattgga
    13201 taacaggttg atcataacct gccgaatacg aatggcatct gctcgaaccg tcggcaggtt
    13261 gggttcgaga atacgcttca tctggatggg tttatctttg atgagaccgc tcatggtgga
    13321 gagtacactg gatgtgatat ccacaatgtt gacttcatcg aaggccagtt ccatcttgcc
    13381 agcttcgatc ttcgaaaggt caagaatgtc attgataaga ccaagcagat gctgacctga
    13441 attatagatg gcggtgagat cttgttgctg tagttctgtt accgggccgt caattccttt
    13501 gatgatcacc cgcgaaaaac cgataatgga gttaagcggt gtgcgtaatt catggctcat
    13561 attggcgagg aattgactct tgaggcggtc gatttcacgc atttccatga ccgcctgttg
    13621 cgagagttca aaggatcggg cattgtcaat ggctaccgcc acctggtctg ccactgtttg
    13681 aagcaccgat atttcgtcat ccgtaaaaga gtttgtagac ttggattgaa cgtcaatgac
    13741 gccgatgacg cgattgccaa ttcggagtgg aatggcggct tcggcagctg tttcaggcag
    13801 gagggggttg aatttgtgaa gcgagtcaat tttgacatca ttcacaacga tagcctgccc
    13861 atccaatgcc accttgccga cgatggattt atcattgacg gggagagagt gcttgttctt
    13921 tttcatttcc gcaccggcgg cgcctgttga ttcgcgtaat aaggcgttga atcctgtctc
    13981 ttccacaata aaaatacttg catggaaaaa gccaaagcgt tcgttgatca agttaacggt
    14041 acgggcaaaa attgtgttaa ggtcaagtgt ggatgtaacg atcttgccaa tttccgaaga
    14101 ggccgccaga tacgcattgc ggcggcgaat ggattcctcg gcctttttgc ggtcggtaat
    14161 atcacgtgca acccagaaca cctgatcatc tttgagtttg gtcaggtttg caaggaacca
    14221 gaatttctct tcaccgatct ggagctcata ttccagttga ttggtctcac ctgtttcaag
    14281 agtatgccga atggcagcta ctgattgatc tgcaacgttt gcaggcaata cctcgtgcat
    14341 gagttttccg agcagatctt ctggcgggcg taccaaacga cctgggtttg ttggcgcaat
    14401 gcgtatataa cggccatctg cgtcgaacac aagcaccacg tcttccattg aggagaaaag
    14461 ggtacgcagg tcggcttctg aacgtgagag cgcttcttct gcattcagac ggctgattgt
    14521 tgctgatatt ggcgaaaggg ttgtttcgat taatgtgatc tcgttcgggt ccagacgggt
    14581 atctttttga gttgtatcca cgccaaggga gccgattaca gtatcgttga cgatgattgg
    14641 aaagatagcc gaataaacaa tacctaatcg ctggaactct tcacgtattt ctggcgggtt
    14701 ggcagggtta ttggcagcat cttcaagaat gtaaggtttt cgagttgaaa taatcgattc
    14761 ggttgatgta tttcctttga tgggaatttc aagccccaat gccgaattat gattatcttg
    14821 ttttgaatgc tccgcaacta cgatgaaact ttgtttttca tcgttgagca gggcaatggc
    14881 cccacgcgaa actgggaatg cttccaccag ttcacgaatc acgagaccca gcgtgttatt
    14941 caggttggtg gcgccagtta gtgtgctaac cacacggttg attagagaaa gttcgtcagc
    15001 gcgcttttct gtttcttgga agaggcgtgt gttttcgatg gccactgcgg cttgttgggc
    15061 aatggtgatg agaacatcac gctggtgtgt agtatagacg ttctcttcct caggatcttg
    15121 cacaacaatc gccccaagga tgcgattacc gagggagaga ggcactccca gccaactctt
    15181 ggctgatgtg gggtttccaa tgatcgtgat gttaattccg agcgcaagag agcgctcgtt
    15241 cacccgattg ggcagaaaca aggtttggtt tgttcgcatg acataatctg tcaaaccatt
    15301 tccgcctgcc ttccgcggtg ctacttctac tttttctcca tcgtcaatca caagcggaaa
    15361 acttacttcc atttgctttt cgtccaacag gcacaggaag aaggaacttg ttctcatcaa
    15421 cttgcttgtg tatttgaaaa ctgattcggc tacattgttg ataccaacca atgttgagag
    15481 ttcgccgctc aatccgttga gtacggcaag ttcttcattt ctctgttttg cgtcgttgaa
    15541 gagctgggcg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttgagtacg
    15661 gcaagttctt catttctctg ttttgcgtcg ttgaagagct gggcgttctg aataccgatc
    15721 gatatctgat tggcgatgtt ttcaagcagg gcgatatctt cctgcttgta agcgaaatag
    15781 tcgtagtttt gaattgaaag acaccccatg gtcttgttaa cagacctcag gggcaggaac
    15841 aacatggttc cataattctt gtttttattg ccgaaagagt cgctgttgat tgatttgctt
    15901 ttaagatctt cgggctttac ggcttccaga agcaactttc cagacttgat gactctccct
    15961 gtgaagtttt cagggttgag tgcctcgttg ggttcctcat acgatttgcc ttcatcaata
    16021 actattgggt aggaaactgt gtttgatgat tcgtcgtaga gagcgacaaa aaatgcatca
    16081 agcgggatca gtttgacgat ttgttcgtag gcggtgttta agacgttttt gagttcgatc
    16141 tgttcggaaa tcgcctgcac aatttcgttt agtgtggcga gttcgttggc tcttttttgg
    16201 gtttcctgaa aaagttgggc gttttccagc gcaagggata attgatcggt aacttgttta
    16261 acaagcaatt cttcatcttg tgaccactgg cgttgtgttg tttcatccaa aatgcgcagt
    16321 gtggcccagt cttggtaacc agtttggatg tttaccgcat aggtggatgc aatattctca
    16381 tgttcttcgg taatgacagg ctcgggagga atcagtgtag atgtgtgaat acgaccctgc
    16441 ctcgggatgg ggcgggtatc cgtttttttg gaagcaggct tcgggtctgg ttttgacgcg
    16501 ggagctgttt ctgtcaccga ggctttagat tcagcctcgt cttttgctgc ctgtttcaag
    16561 ctggaggaag cttcttccag atttatgtct ttgaatagct tgttaagccg tttgtctatt
    16621 ttcggtttct ttggcatatt cgatcaactc ctgcgcgagc acacggtatt ggattgaacc
    16681 acgtgcgccg gggcggtagt gggaaatggg caaaccggcg atggggcttt ctcgtaattt
    16741 tgtgtcagtt tcgatcacgg tgctgaacac accttcacca aaagtagaac gtagctggtc
    16801 gtggatgttg cgatgtgtgc gattacgttt gtcgagcatg gtgacaagta cgcggtaggc
    16861 caggttgggg ttttcgccct cgcgaattct tcgtatcaag gtcatcatgt tccgaagtgc
    16921 gtatgccgaa aaatattcgg cttgtgtagg gataatgaga aggtcggcgg cgataagcgc
    16981 attttgtgtg atggcgccca atgcaggcgg acaatcgaac aggatatagt cgtaatgcaa
    17041 attaggtgcg ctggcaattg cgcgttgtaa tgttgtggtg taactggtgc gtacgggtaa
    17101 aaattgttcg gcggtttcga tgcgagaatt ggatgggatg aggtcgaggt tttcgatttc
    17161 agtttttcgc agcgcattca ggagaggtat gttgtcgagt attacttcgc tggatgtgat
    17221 tgcagattcg cctggctcaa gacccagggc gagagtgagg ttggcttggg catcaaggtc
    17281 taccagcaaa acacggtttc ccatgtccac cagggcggcg ccgagtgata ttgtggaggt
    17341 ggtttttgct acaccgcctt tttcattgga tatagcgatt tttttgatca ttgtttacca
    17401 tccagtctat ttatcaggag gaggggcgtt ttttctggga acgatctcga ctcgggatgc
    17461 tcccaatgcg cgttgaagtt cctgtacagc agttctaatc atttcttgcg ggtcgtttgt
    17521 gccgcgaatc ttggtggtga tttctgttac caaccgttcg cgcgaagcac gtgacgatgt
    17581 ttcttcgaag aggcgggcat tatccagggc gaggccaagg cgttctgcca cggcattgac
    17641 aatgttaatc tcatccctgc tccaggctct atcttcctta tttgggcgga tgtgaacggt
    17701 gccaatcact tgatcgcgca gttgaatggg gatggacagc gcgttctcgt ctttttcggt
    17761 tttggcgact acttccggtg tcttttctcc agtaatgagt tggccgccgg tcagggtttg
    17821 ataatacccg aaaatctcag aggcagattt gttttgccat gccccggcaa ggtattcacg
    17881 gaaagaggat tgtgcttctg cgagtgcctt tttagtttcg tccaacaggc ggacattgtc
    17941 aatggcaatg gcgatttggt cggctaataa tcccagaatg gagatgtcgt tctgggtaaa
    18001 tgcgttgggg aaagtgcttt gtacgtcaag tgctccaatg attgtacctc gggcaatcaa
    18061 aggtaatgcc atttcggaat gcgtttcagg caggtctgga ttattgaaaa atgtcgaatc
    18121 tgcgcctgta tcaagagcga tacgggcaat gccggtggct gtcacattgc ctacaatacc
    18181 gacctggccg acttcaagtt tatgttgacg tttcaacatt acctgtccgc ctgggctgtt
    18241 ggctgcacgt agaacggcaa atttcccatt ttcatttagc agaaagatac cgacatgata
    18301 aaagccaaag cgttcgctca cagtttttgt aattaatggg agcaggcttt cgaggtcttt
    18361 ttctgccgaa atgtagcctg aaatttccgc aatgatttga agctcatcag cgcgtttttt
    18421 gctttgttct gtgagctttt cgagttcatt tgtcctttgt gccaattcga gtgtgcgctg
    18481 ttcaacacgg ttttcgagac ttgcgataaa gcctcggatc tgcgatgcca tttcgttgat
    18541 ggcacctgca agaaggccga tttcgtcatg agtttttatt tcagcctgct gtgaaaagtc
    18601 gcctgcagag attttttctg ccacacttgt caattgttgc aatggatttg tgaacacatt
    18661 ggagactgcc actgcactta ctgctgcaat aaaaacgatt gcaatactga tgaggacggt
    18721 gttgcgggtt tgtccgcttg tggcttttg
//
LOCUS       DAOQLC010000084        18643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1034_length_18643_cov_23.005434, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000084 DAOQLC010000000
VERSION     DAOQLC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18643
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1034_length_18643_cov_23.005434"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PL000_14290"
     CDS             complement(<1..272)
                     /locus_tag="PL000_14290"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC90121.1"
                     /translation="MKSFFSKLLPPLRTDAPRKRKTLGQSLIEFAIALPVLVLILTAM
                     MEFGFILNFYLSLLDATRGAARWASLANPLNADGTDDMDYYITTAE"
     gene            complement(576..1838)
                     /locus_tag="PL000_14295"
     CDS             complement(576..1838)
                     /locus_tag="PL000_14295"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HNC90122.1"
                     /translation="MNRKLAANFRNSERGQAIIVIVFAIIGIVGISSLAIDGSNALID
                     RRKSETAASAAALAGALARIEGRDWRESALAAAKMNGYDNDGENSIVELNTPPINGPY
                     AGDAEYIEVIVTSNMRTYFGSIIGIPVVTSVGYAVAQTKPAEYGQMFDGYALVSLAPH
                     SECDKKKAFWLHNEPTIVLEGGGIFVNSDNDDCAFLQEGSGSIRVQDESPITVVGGAS
                     VQKAQLISPAIVQPGAIPISYPPAFQMPKIGCGSKIATIDLVTGTMTSGNYEEPGDFP
                     PEGIHDLESGIYCISGDVVIGGGTRLTGNGVTLIVEQGEVRIAAEAEVDLSAPKNGNA
                     AGLLIYMPLDNHSRIALNGNADSNFTGTILAPSADIRLNGPDSKHGFHSQIIGYYIEV
                     DGQSKIVIKYEDEDNYDAYKMPEVILVQ"
     gene            complement(1835..2299)
                     /locus_tag="PL000_14300"
     CDS             complement(1835..2299)
                     /locus_tag="PL000_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC90123.1"
                     /translation="MKVKADHRERGQSLVELAVSLPIILLLLLGTLDFGMAIFSYSML
                     RDAAQEGAFYGSFNPASKEAIENRARYISPRGEDEIFSSPVDLTNPDLVQVEVDTLGQ
                     ACQGITDETINSIRVTVTYNYPIMMPIIGPLFGSTIPLTGTASNIILQPACP"
     gene            complement(2315..3457)
                     /locus_tag="PL000_14305"
     CDS             complement(2315..3457)
                     /locus_tag="PL000_14305"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HNC90124.1"
                     /translation="MKRFIFSKNNKTRAQAMVEFMLALPILLIILYGLIEVGRLAFIF
                     SSTANASRQAARYGAASGEINDVAFYQDCEGIRSVAEESAFIIEFENINITYDRGVDE
                     DGNQVPISGVDPSPDEDTCPIPPGVIRNGDRIIVQVSAIYEPIISGPVEPMEIVSSSA
                     RSFLISVPIVGSALPTGFSPETSTPSQVPTSSLFTSTPTLYVSPTYTFIPPSLLTNTP
                     NPNFTPTLTREPTLTFTPSRTPLPTYTPSVTPTAISCNGITGVTHGPLEFKDNVMKMS
                     IDNQTGHTLVAANIYLEWNHDTGHGEGNDLGLILNRVTFAGQAWDGALQAPSAYIPAF
                     YPTIPPGISTISFSFHQNYLLTDGTERIIVNIGTPGCINYPVDSSH"
     gene            complement(3567..4355)
                     /locus_tag="PL000_14310"
     CDS             complement(3567..4355)
                     /locus_tag="PL000_14310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNC90125.1"
                     /translation="MSITAIISILFGWLAGVLVNYLADVLPHTRRFSQPACLHCNAPF
                     AWQDYLLFKSCPNGHPRSTRTWVVQFVILAISIYIWLNPPSKIGYGLGMILLVYFGIV
                     FVIDLEHRLILHPTSIFGALFGLIIGTIAHGIAPTLWGGLGGFVIMMVLYYFGVLFAR
                     VRTRRLIAAGQEPDDEEALGAGDVILITILGFILGWPLIWFGFLLGVLLGGFFSLLII
                     VWLVVVRKYRQNAMMMFIPYGPYFVTSAFLVVYFPKFLALLVPG"
     gene            4486..5766
                     /gene="murA"
                     /locus_tag="PL000_14315"
     CDS             4486..5766
                     /gene="murA"
                     /locus_tag="PL000_14315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC90126.1"
                     /translation="MEEFVIEGGTPLHGEVTPSGNKNAALPLLAACLLTSEPVVLKNV
                     PQIRDVQVMRKLIESLGSQVEDLDSNTWRITTKDLVASHLDPDLCRRIRASILIAGPV
                     VARAGTLRLPPPGGDVIGRRRLDTHILALRALGAKVTYDRVFEFEADQLHGADILMDE
                     ASVTATENAIMAAVLAKGQTQIRNAASEPHVQEVCQFLNILGAKIEGIGSNTLHITGV
                     PQLKGGEFTIGPDLLEVVSYVGAAVVTRGEIRIRNAGVQHLEMVGQVFHRLGVHWNVE
                     GNDLFVPADQSLVIASDLDGAVPEIKTNVWPAFPTDLMSIAITVATQASGSVLFHDWM
                     FSGRMYFTDKLVGMGARIILCDPYRVLIQGPTRLYGEKLESPDIRAGMALLLAALAAK
                     GTSKIRNISQIERGYEKVDEKLRMLGASITREQE"
     gene            complement(5853..6467)
                     /locus_tag="PL000_14320"
     CDS             complement(5853..6467)
                     /locus_tag="PL000_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90127.1"
                     /translation="MLKRILPILLALSVLLTACGQATPTAAPVDVEGTAVAAAWTMVA
                     ATQMAIPTATPLPPTETPAPTVPPTFTPPPLLELPTATLFNLPTPTAVSAADDCNHPL
                     NVGEAGETNPMRIENASGGTIAWLSLNLSKNAFGQCGAMSWSNIGNGAKFKVDLPKGI
                     WWAYAGITFKNGSSSNASGSFEIRLGDEDLLRIIVGKDVITVHP"
     gene            complement(6502..7194)
                     /locus_tag="PL000_14325"
     CDS             complement(6502..7194)
                     /locus_tag="PL000_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90128.1"
                     /translation="MKKYFFSFLVLSFALSACMPALPAFFQSEPTSSPIPLPTSEENL
                     QATAAVIAQQTLEALPSPTLEPSHTPIVPTQTSTSTQQPTPTETVNPILLTLTATLGT
                     GTVSPGTPGTPGASTGTPNPAFSATPTETLHPQLYGTMPPSLPSGYVTFINESRAEVY
                     VSLQCTTKEGFVTIIEYPVGRRVSTDAPAGEYIYVVWVGGNKITGEFRLGASQTLTMT
                     IFKDRVAIKSKL"
     gene            complement(7224..7568)
                     /gene="trxA"
                     /locus_tag="PL000_14330"
     CDS             complement(7224..7568)
                     /gene="trxA"
                     /locus_tag="PL000_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNC90129.1"
                     /translation="MSSEPIHVTDEAFEKVVMQSTLPVIVDFWAPWCNPCKMIAPTLE
                     KIAVEQEGKLVVAKINTDEHPQWMQKFGIQGIPTLLFISGGKVVHRQVGALPERMLRD
                     VVNQFMEVISNN"
     gene            7712..8623
                     /gene="ftsY"
                     /locus_tag="PL000_14335"
     CDS             7712..8623
                     /gene="ftsY"
                     /locus_tag="PL000_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNC90130.1"
                     /translation="MNNLFSRWKESLTRSSKAAFGQLASILGTSEITEETWDDLEALL
                     LQADLGLETTSSVLDSLKRITRTEGLVRANELSATLRQELRARLSTPPALSLNDKPSV
                     VLVVGVNGSGKTTTIAKLSSRFTAEGRKLLLGAADTFRAAAVDQLQVWGDRLKVEVIA
                     GAFESDPGAVAFNAVQAGVARGVDIILIDTAGRLHTRFNLMEELKKVNRVVGKALPGA
                     PHQVWLVLDATTGQNALQQARSFKEAVQVSGVILSKLDSSARGGMAFAIQRELGLPIL
                     FAGLGEKPEDLQPFDPDAFVDGILSNA"
     gene            8632..9724
                     /gene="prfB"
                     /locus_tag="PL000_14340"
     CDS             join(8632..8697,8699..9724)
                     /gene="prfB"
                     /locus_tag="PL000_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114679478.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNC90131.1"
                     /translation="MQDLLNRLQAANDLTQQLMVRLDLAGKEIQLEQLEKDIGQPEFW
                     NDSIGAQKVMKTVSSLRDEVGFWNSTKQHLHDLTDLVSLGDESLRSDLEGEITKLEAS
                     LDKKSFNTMFSGPFDHDDAILAIHAGAGGTDSQDWAGMLQRMYLRWAESQGFGTEVLD
                     FTPGEEAGIKSITIAVSGEYAFGHLRSEKGVHRLVRLSPFDAAHRRHTSFALIEVLPQ
                     VSMEEADVEINPSDVKIDVFRSSGAGGQNVQKNATAVRLTHLPTGIVVTCQNERSQSQ
                     NREFAMKILRARLLELKTAEKEEERAILRGEYTKAEWGSQIRSYVLHPYQMVKDHRTD
                     HETGNSQAVLDGDLNGFMEAFLKSSFPEG"
     gene            9861..11246
                     /locus_tag="PL000_14345"
     CDS             9861..11246
                     /locus_tag="PL000_14345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC90132.1"
                     /translation="MSVFSVGVLAFAAFIVGRVGRGRELTLLAASVFFIYWLQPAQTI
                     SSLSFWLPTITLALSIFCWVLVAPPEIRVWGKTWPAVLVIVGTILLVDLSQFLGSKIF
                     SATPRLWIVVLALAAFFALTFFTEKLPRRPIFFSAVLWGIVLLFIFLKTPYFLNSFLN
                     SIFTPSKEGVLFSWLGFSYVAFRLLHTLRDRQTERLPALTLAEYLNYVIFFPSFAAGP
                     IDRAERFVLELRNPVALAEQDWVQAGTRFFIGLFKKFVIADALALVSFNDALAEQVRS
                     PVWIWFLLYAYSLRIYFDFSGYTDIAIGLARILGIRLPENFAAPFLKPNLTQFWNSWH
                     MTLTHWFRAYFFNPITRSLRTKQLPVPLIIFTAQLSTMVVIGLWHGITWSFFFWGIWH
                     GLGLFIQNRWSEWIQSRMETPPPPHRQLVLNVIGMFLTFNFVSLGWLFFTLSSPLLAL
                     QVLMKLFGATI"
     gene            11243..12361
                     /locus_tag="PL000_14350"
     CDS             11243..12361
                     /locus_tag="PL000_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90133.1"
                     /translation="MKVNPLRVLVKALILYVVANILFAIYNPPIGRISVYNWLVPGRL
                     RFPYERAENVNLGYNISVFQDLDAAFASHVIADGEKPADEYRVILVGDSSIWGFASNS
                     TETLSAKLNDLELTACDGRRMRFYNLGYPWSFIFKDLLVLEHARVYEPDMVLWLFTMR
                     GLTGSDKDIVDFFEGELIVDAKRVQAKYGLEDYTSELTTQTFFEQTIIGERKKIKTDI
                     TLQMYGFLWAATGIDHHLQTWLPWKDTLENSIEYRHYRTPEEWPQLQDDFMLDVLDAA
                     HTMMGDTKIVAVNEPIYISSGSNSEVRYNKLYPRWAYDRYREFMQGWMAENQYPYLDM
                     WNVIPQEEFSDFPVHLYPTGERLLAESLAPEVLKFSCP"
     gene            complement(12409..13191)
                     /locus_tag="PL000_14355"
     CDS             complement(12409..13191)
                     /locus_tag="PL000_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNC90134.1"
                     /translation="MTTPDSAPAVALEPKTKLSGKILKTTLAGALVDIGQSLPGVIHI
                     SQLQQDAVNKVEDVVKEGQTVEVWVRRVKKDRIELTMIEPLGYEWKEIVPDMTVKGKV
                     VRIETYGAFVDFGAERPGLIHVSELTHGYVKTAGEVVKEGDEVEAKVLDVDRRKRQIR
                     LSMKALLPEIVEEEKPEREERSKKGDKPKGKKGKKQEEEAYEMEVEVSNEPQFTAMQI
                     AWQQALERSKGRGKVRTKSYKSASDEKEELFNRTLEKRASSN"
     misc_binding    13335..13618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13676..16828
                     /gene="ileS"
                     /locus_tag="PL000_14360"
     CDS             13676..16828
                     /gene="ileS"
                     /locus_tag="PL000_14360"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNC90135.1"
                     /translation="MFKAVNPKLNVPLMEETILKLWKKQNVFKKTVEQRKDGPEYVFY
                     EGPPTANGRPGVHHVLARAFKDMFPRYKIMRGYHVSRRGGWDTHGLPVEIEVEKQLGF
                     TNKQQIEEYGIDKFNELCRNSVFTYIQDWEKLTDRIAFWVDLETAYVTYENEYIESVW
                     WILKNFWEKDLLFKGYKIVPYCPRCGTPLSDHEVALGYDDAVDPSVFVRMPLVDKPDT
                     SLLVWTTTPWTLPANVAVAAHPEVDYVTIERDNDGTKEKLILAKNLVEKIFAGEEVKV
                     VDSFKGKKLKGLKYKPLFTFVPVDKPAYFVVLGDFVTTEDGSGLVHQAPAFGAEDMEM
                     AKQHDLPILLTVQPDGTFIPEITPWRGIFVKDADPQIIQDLDARGLLFRAGTLTHTYP
                     FCWRCKTPLLYYARESWYIRTSAKRDRLVELNKTINWVPDHIKNGRFGNWLENNIDWS
                     LSRERYWGTPLPVWECENQECKHRECVGSVAELSEKAGKDLTELDLHRPHVDNVHWKC
                     VECGGKMSRVKDLIDVWFDSGSMPYAQWHYPFENQDKFKQQFPADYICEAVDQTRGWF
                     YSLHAISTLLNDDVSFKNVICLGLILDGEGRKMSKSIGNIVNPWDVLNVHGADAFRWY
                     LYTASPAGQERRFSSDLVGDVIRSFTLTLWNVYSFFVTYANLDKPQALTITAPTNDLD
                     RWLLSELNVLVRDVTNAYENYDATNATRPVEKFVETLSTWYVRRSRRRFWKNDSDSDK
                     QAAYSTLYTALVTVAKLIAPAMPFVAEELYQNLVRSVDETAPESVHLAEWPKVMEEFI
                     DESLNRDMNLVMKLVSLGHAARQKANRKVRQPLAEAAFSVGNNVERNALSANMDVIAD
                     ELNVKQVRLLDASTEALTHTVKPLPKQLGQKYGNKFPALQKAILAMNAEEVAKILHSG
                     APLNVTVDGETYGILSDEVEVKALAKEGFAVAEDGAYVAALVTDLTPELIAEGQAREF
                     VRRVQDLRKTADLDVADRVELFIEATAGLRSAIEAHEGYIKAETLTVKLSFANPPATA
                     SVVEDEFDGEKVKVGLVKSK"
     gene            complement(17009..>18643)
                     /locus_tag="PL000_14365"
     CDS             complement(17009..>18643)
                     /locus_tag="PL000_14365"
                     /inference="COORDINATES: protein motif:HMM:NF027421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HNC90136.1"
                     /translation="QFFFSGRSLYLLVFDTGKTLEQNNIEYWLTLINAYSQNAPVILV
                     GNKCDQHEMDVEKRRLREKYPNIRGFVGTSASTGEHIAELRQKIQEEVRAMPQVRVLF
                     SADYLAVKDEMETLSAAHISHEEFVRVCERHGVSDAKDQDTLLDLLHDLGTVLYFRDA
                     DGNPLLPALGVLNPNWVTRGVYLIVTNPALKDKYKGKLSKAYLREILSAPEYSRHIDD
                     IAKLLERFELCYRADDSSYWLPSLMAKEIDEDLGNLKSSAMKFEYGYPELPKSVIVRF
                     IVKTHARILDNKVWRSGVVLRLDGNTALVQADEEDKCIRIFISGEESTRRDALAILRA
                     HFDDIHTMFATPPEAFIYPPQYPNVQLRFDKMKNLLKTGTTQIPELDGDKPVTINLRE
                     LLDGFVTPQERRKDEEREKQEGKRGDTYITNNNITMGNMHGSSLTLGDGNTSTVSVQN
                     SFNTFPAELQNTLTELMKASETLLQKAEESEHTEEIKDELESLQTEAKKSKPKKERIK
                     VTVEGLAQAAKNLNEIGKPVLELAMTVIKLINNLPA"
BASE COUNT         4308 a         4394 c         5103 g         4838 t
ORIGIN      
        1 aattcggcgg tggtgatgta gtaatccatg tcgtcggtgc cgtctgcgtt aagtggattt
       61 gcaaggctgg cccagcgtgc ggctccacgg gtggcatcca aaagcgacag atagaaattc
      121 agaataaaac cgaattccat catggcagtt aatattaata ccagcaccgg tagagcaatg
      181 gcaaactcaa tcaaactttg accaagggtt ttgcgtttgc gcggtgcatc agtacgtaaa
      241 ggcggaagta gttttgaaaa aaacgatttc atattattct cgataataga ttattttagt
      301 gcttattcaa tcaaaatata catccgacat aagtacggga aaacaaacat ctttggctgt
      361 gatgctgggg gaatgaaaag cgcgtttttt ggcgtttttt cgatgcaaat acattatatc
      421 tcattcaaaa gacgcctgtc actatgcaag aatgctgcaa cgctaacaga tttgtaagaa
      481 caagtcagtt ttggagctat ttccccgtga atctgacttt tgtaggctga aaaactttta
      541 caccaaggac tgcaaacgta aggctgaaag ctcgcttatt gaaccagaat cacctccggc
      601 atcttatagg catcatagtt gtcttcatct tcatatttga tgacgatctt actctgaccg
      661 tccacttcaa tgtagtagcc gatgatctgg ctatggaagc cgtgcttgga atctgggcca
      721 ttcaggcgga tatctgccga aggggcaagg atggtgccag tgaaattgga gtcggcattg
      781 ccgttcagtg caatgcggct gtggttatcg agcggcatgt agatcaatag accagccgcg
      841 ttgccgtttt ttggagcgga taaatccacc tcagcttcag ccgcaatacg aacttctccc
      901 tgctcgacga tcaacgttac accatttcca gtcaggcggg ttccgcctcc gatgaccacg
      961 tcaccactga tgcagtaaat gccgctttca agatcgtgaa tgccttcagg agggaaatca
     1021 ccgggttctt cgtagttgcc agaggtcatc gtaccggtta ccagatcaat ggtggcgatt
     1081 ttcgaaccgc atccgatctt gggcatttgg aaggctggcg gataggaaat gggaatcgca
     1141 cccggttgga caatggctgg gctgatcagt tgggcttttt gtacactggc tccgcctaca
     1201 acagtaattg ggctttcatc ctgcacacgg atgctgccgc tgccttcctg taaaaaggcg
     1261 caatcatcat tatctgagtt gacaaagatg ccgccgcctt ccaaaacgat ggtgggttcg
     1321 ttatgcagcc agaaggcttt tttcttgtca cattcactgt gcggtgcaag gctgacaagc
     1381 gcgtagccgt cgaacatttg gccatactct gcgggtttgg tctgagccac tgcataccca
     1441 acactggtga cgacggggat tccgatgatg gaaccaaaat aggtgcgcat attcgatgtc
     1501 acgatgactt cgatgtactc ggcatcccct gcataggggc cgttgatggg gggagtgttc
     1561 agttccacaa tgctgttctc gccatcgttg tcataaccgt tcatttttgc ggcagcaagg
     1621 gcagactcgc gccaatccct gccttcgatg cgggcgaggg ctcctgccaa agcggcggca
     1681 gaggcggcgg tttcagactt tcggcggtct atcagggcat tgcttccgtc aatggcgagg
     1741 gaggatatgc cgacaatgcc aataatggcg aacacgatca caatgatcgc ttgtccgcgt
     1801 tcgctatttc taaagttggc tgctaatttt cggttcatgg gcatgcgggt tggaggatga
     1861 tgttcgaggc tgtaccagta agcgggatgg tggatccgaa taatggtcca atgatcggca
     1921 tcatgatcgg gtagttgtat gtcacggtga cgcgaatgct gttgatcgtt tcatctgtaa
     1981 taccctgaca ggcctgccca agtgtatcca cttccacttg aacaagatcg gggttggtga
     2041 ggtctaccgg agaggagaat atttcatctt caccgcgcgg tgagatgtag cgggcgcggt
     2101 tttcaatggc ttctttgctg gcagggttga acgagccgta gaaggcgcct tcctgggccg
     2161 catcgcgcag catggaatat gaaaagatcg ccatgccaaa atccagcgtg cccaataaca
     2221 acaaaaggat gatgggcagg ctgacggcca gttcaaccag actttgtccg cgttcgcggt
     2281 gatctgcctt cactttcatg gaagttgaaa tcctttagtg actcgaatcc actgggtagt
     2341 tgatacaacc aggggtgccg atattcacga taatgcgttc ggttccatcg gtcagaagat
     2401 aattttgatg gaagctgaaa ctgatcgtgg atattccagg tggaatggtc gggtaaaagg
     2461 cggggatgta agcagatggt gcttggagtg cgccatccca ggcctgacct gcaaaggtaa
     2521 cccggttgag gatcaaaccg aggtcgtttc cttcaccatg acctgtgtca tgattccatt
     2581 caagatagat atttgccgca accagtgtgt gaccggtctg gttgtcgatg ctcatcttca
     2641 taacattatc tttgaactcg agcggaccat gtgttacgcc ggtgatgccg ttacaactga
     2701 ttgccgtggg ggtgacgctg ggcgtgtagg ttggcagggg tgtgcgtgac ggtgtaaagg
     2761 tcagggtggg ctcgcgggta agcgtgggag tgaaattcgg gttgggtgta tttgtcagca
     2821 gtgaaggcgg aataaaggta taggttggag aaacgtacaa tgtgggagtg gaggtgaaca
     2881 ggctgctggt cggcacctgt gaaggagtgc ttgtttcagg agaaaacccg gtaggcagcg
     2941 ctgacccaac gatggggaca gagatcagga aggagcgcgc gctggaagac acgatttcca
     3001 tgggttcgac ggggccagaa ataatcggct cgtatatggc agacacttgc acaatgatgc
     3061 ggtcaccatt gcgaatgacg cccgggggaa tggggcaggt atcttcgtcg gggcttgggt
     3121 caacacccga tatcggaacc tggtttccgt cttcgtccac gccgcgatcg taggtgatgt
     3181 tgatattttc aaattcgatg ataaaggccg attcttcagc cacactacga atgccttcgc
     3241 aatcctgata aaaagcgaca tcgttgatct cgcctgaggc agccccatag cgggcagcct
     3301 gacgggatgc atttgcagtg gatgaaaaga tgaatgccag tcgcccaacc tcgatcaacc
     3361 catacaggat gatcagcaaa atgggtaaag caagcatgaa ctcaaccatt gcttgtgcgc
     3421 gggtcttatt atttttggag aagataaatc gtttcatatg gccagctttt catctaactt
     3481 gattttgggg cgggggattt gtatgtacgt ctaattttat attactggtt tgggccgtca
     3541 aaagccatcg gtctaagttc ctatggctat ccgggcacaa gcagcgcgag gaattttgga
     3601 aaatagacaa ccaaaaaggc gcttgtcaca aaatatgggc catagggaat gaacatcatc
     3661 atggcatttt gcctgtactt tctaacaacg accagccaga caatgatcaa caaactgaaa
     3721 aagccgccaa gtaaaacacc cagtaggaaa ccgaaccaga tcagcggcca gccaaggata
     3781 aaaccaagaa tggtgatgag gatgacatct cctgcgccaa gcgcttcctc gtcatcgggt
     3841 tcctgcccgg cggcaatgag gcggcgtgtc ctgacccggg cgaacagcac accgaaataa
     3901 tacaaaacca tcatgatcac aaatccgcca agtccgcccc atagggtggg cgcaattccg
     3961 tgcgcgatcg tgccgatgat caacccaaat agggctccaa aaatactggt ggggtgaaga
     4021 atgaggcggt gttccagatc gatcacaaag acaatgccaa aataaaccag caaaatcatt
     4081 ccaagcccat agccgatctt ggatggcgga ttaagccaga tgtaaatgct gatcgccagt
     4141 atcacgaatt gcacaaccca ggtacgggtg ctgcggggat gtccgtttgg gcaggacttg
     4201 aataataaat aatcctgcca ggcaaatgga gcgttgcagt gcagacaagc tggttgactg
     4261 aaacggcgtg tatgtggcag gacatcggct aggtagttta caagcactcc agcaagccat
     4321 ccgaacagta tggatatgat tgcagtaata gacatttcaa atgattataa ttccaattga
     4381 taccatcgtc caaaacccct tttggtgtaa gtgaattgta atgttttctt gaaatgtttt
     4441 atactacctt tcaattctga aggcgttctg aggagaccat atcaaatgga agaatttgtg
     4501 atcgaaggtg gaaccccact tcatggcgag gtaactccct ctggaaataa gaatgcagcc
     4561 ttgcccctgc tggcagcttg cctgctcaca tctgagcctg ttgttttgaa aaatgtgccg
     4621 cagattcgtg atgtgcaagt catgcggaaa ttgatcgaga gtcttggttc acaggtggag
     4681 gatttggatt caaatacctg gcgcatcacg acgaaggatc tagtcgcatc tcatctggac
     4741 ccggacctgt gtcgtcgaat ccgcgcttcg attctcatcg ccgggccagt ggttgccaga
     4801 gcaggaaccc tgcgtttgcc acctccaggc ggggatgtga tcggtcgccg acgtttggac
     4861 acacacattc tggctttgcg tgctttgggc gcaaaagtga cctacgaccg tgtttttgaa
     4921 tttgaagcgg atcaattgca tggtgctgat atccttatgg atgaagcaag cgttactgca
     4981 actgaaaatg ccatcatggc ggctgtgctt gccaagggcc aaacccagat tcgcaacgcg
     5041 gcatccgaac cgcatgttca ggaagtctgc cagtttttga acattctggg ggcgaagatc
     5101 gaaggcattg ggtcaaatac cctgcatatc acgggtgttc ctcaattaaa aggcggcgaa
     5161 tttaccattg gccccgacct tctggaagtg gtcagttatg tgggggcagc ggttgtcacc
     5221 cgtggtgaaa ttcgcatccg caatgctggg gttcaacatt tggaaatggt tgggcaggtc
     5281 tttcatagat tgggcgtcca ctggaatgtg gagggaaatg acctgtttgt ccccgctgac
     5341 caatcccttg tgattgcctc tgatcttgat ggtgctgtgc ctgagatcaa aaccaatgtc
     5401 tggcctgctt ttcccaccga tttgatgagc attgccatca cagtggctac ccaggcttct
     5461 ggttcggtgc ttttccatga ttggatgttt tcgggccgaa tgtattttac ggataaactc
     5521 gtcggcatgg gggcgcggat cattttgtgt gacccgtatc gggtgttaat tcaaggccca
     5581 acaaggttgt atggagaaaa acttgaaagc cctgatattc gtgccggcat ggctttactg
     5641 ctggctgcct tggccgcaaa agggacttca aagatacgca atatcagcca gatcgaacgc
     5701 ggctacgaaa aagtggatga gaaactccgc atgcttggtg ccagcatcac ccgtgagcag
     5761 gaataagaaa tgaacaaaaa agaagccgcg agtttttcgc ggcttctttt ttgttttggg
     5821 gaatacggac tgaagatccg cactttgcag gattacgggt gaactgtgat gacgtctttc
     5881 ccaacgataa ttctcagcag gtcttcatcg ccaaggcgga tttcaaaaga gccagaggcg
     5941 ttactgctgg agccattctt gaaggtaata ccggcgtaag cccaccagat acccttgggc
     6001 aggtcaacct tgaattttgc cccgttgcca atgttgctcc aggacatggc gccgcactga
     6061 ccaaaggcgt tcttggaaag attcaaagac agccaggcaa tggtaccgcc gctggcattt
     6121 tcaatacgca tcggattggt ttcgccagct tcacccacat tcaaaggatg attgcagtca
     6181 tctgcggcag aaaccgcggt gggggtgggc aggttaaaca gagtagcggt cggcagctcc
     6241 aacaatggcg gaggggtgaa tgtgggggga acggttggag caggtgtttc tgtaggaggc
     6301 aggggggttg cagtagggat cgccatctgg gtcgcagcga ccattgtcca tgcggcggcc
     6361 acggctgtgc cttccacgtc tacgggggcc gctgtgggtg ttgcttgccc acaggccgtc
     6421 aacaacacgg aaagcgcgag caggatgggt agaattcttt tcaacatttt ttctccattt
     6481 attttttgtt ttgaatagga cttataactt ggacttgatg gcaactcgat ctttgaagat
     6541 ggtcatggtc agcgtttgtg aagcgccgag acggaactct ccagtgatct tgtttccacc
     6601 gacccacacc acatagatgt actcaccggc tggggcatcg gttgaaaccc tgcgcccaac
     6661 ggggtactca atgatcgtca caaaaccttc cttggtggtg cattgcaatg agacatacac
     6721 ctcggctctt gattcgttga tgaaggtaac gtatccggaa ggcaggctgg gcggcattgt
     6781 accatacagc tgcgggtgaa gcgtttcagt cggggtggca ctgaaagcgg ggttgggtgt
     6841 acctgttgac gctccgggag tccccggtgt tcctggggat actgtgcctg tgccaagggt
     6901 ggcggtcagt gtaagcagaa tggggttgac cgtttctgtg ggcgtcggct gttgtgtgga
     6961 ggtgcttgtt tgggttggta cgatgggggt gtggctgggt tcaagagtag gggaaggcag
     7021 ggcttcaagg gtttgctggg caatcactgc agcagttgcc tgaaggtttt cctcagaggt
     7081 cggcagggga atggggctgc tggttggctc tgactgaaaa aaagcaggca gggcaggcat
     7141 gcacgcgctt aaagcgaagc taagcacgag gaacgaaaaa aaatattttt tcatggcctg
     7201 tttccttgat cctgctcccc caactaatta ttgctgatca cttccataaa ctggtttaca
     7261 acatcgcgta acattcgctc gggtaatgca ccaacctgac gatgcaccac tttgccgcct
     7321 gaaatgaaca gcagggtggg gatgccctga attccgaact tttgcatcca ttgcgggtgt
     7381 tcgtctgtgt tgatctttgc cacaaccaat ttgccttcct gttccacggc aattttttca
     7441 agcgtggggg cgatcatttt gcatgggttg caccacggcg cccaaaaatc cacgatcacc
     7501 ggcagggtgg attgcattac aactttttcg aaggcttcgt ctgtgacatg aatgggttct
     7561 gaagacattg ttatttttat tcctttcaac ctttcgtctt ttgtgcgcat gtgaaaatgt
     7621 cagtcaacat gcaacacagc tgcccatagg gacaaaaaag tataacataa aatccatgat
     7681 atataatgac cgtagggtat aatcgccgct catgaataac cttttttcac gttggaaaga
     7741 atcacttacc cgttcgagta aggctgcatt tggtcaactc gcatccatcc ttggtacatc
     7801 tgagatcaca gaagaaacat gggatgatct ggaagctctg ctgcttcaag cagacctggg
     7861 attggagacc acctcctcgg ttttggattc acttaaacgc atcacacgga ccgaaggact
     7921 ggttcgcgcc aatgaacttt cagcgaccct tcgacaggaa ttgcgagccc gcctttccac
     7981 cccacccgct ttaagcttga atgacaaacc ctctgttgtg ttagtggttg gtgtgaacgg
     8041 ttcaggcaaa acaaccacca ttgccaaact ctcgtctcgt tttaccgccg aaggccgaaa
     8101 actcctgctc ggcgccgcag ataccttccg tgccgcagcg gtggaccagc ttcaggtttg
     8161 gggtgacagg ctcaaggtcg aagtgatcgc aggtgctttt gagtccgatc ctggtgcggt
     8221 ggctttcaat gccgttcagg ctggagtcgc tcgaggtgtg gatatcatcc tgatcgatac
     8281 agctgggcgc ttgcacaccc gtttcaattt gatggaagaa ctgaagaagg tcaaccgtgt
     8341 ggtgggcaag gctttgccgg gggcgccgca tcaggtgtgg ctggtgttgg acgcaaccac
     8401 cggtcagaac gctttgcaac aggcccgttc atttaaagaa gcggtgcagg tttcaggcgt
     8461 catcctctca aaattggatt cgtcagcacg tggcggcatg gcttttgcca ttcaacgcga
     8521 acttggcctg ccgatcctct tcgctggttt gggcgaaaag cccgaagatt tacaaccctt
     8581 tgaccccgat gcctttgtgg acgggatttt atcgaacgcg taggagctat tatgcaagac
     8641 cttttgaatc gcttgcaggc cgcgaatgac ctgactcaac aactcatggt gcgtctttga
     8701 cttggcgggc aaagaaattc aacttgaaca actcgaaaaa gacataggac aacccgagtt
     8761 ttggaacgat tcgatcggcg cgcaaaaggt gatgaaaacc gtttcgagcc tgcgcgacga
     8821 agtgggattt tggaattcga caaagcagca tcttcacgac ctgaccgacc tcgtgagcct
     8881 tggcgatgaa tcattgcgca gcgatctgga aggtgagatc accaaacttg aagccagcct
     8941 tgataaaaaa tcgttcaata cgatgttctc gggaccattc gaccatgatg atgccatcct
     9001 tgccattcat gcaggagcag gtggaaccga ctctcaggat tgggctggca tgctgcaacg
     9061 catgtatttg cgctgggccg aaagccaggg atttggcacc gaggtgctgg atttcacccc
     9121 cggcgaagaa gcaggcatca aaagcatcac cattgccgta agcggagaat atgccttcgg
     9181 gcatttgcgt tctgaaaaag gcgtgcatcg tttggtgcgt ctttccccat ttgatgcggc
     9241 gcatcgcagg catacttctt ttgcgttgat cgaggtcctg ccgcaggttt caatggaaga
     9301 ggctgatgta gagatcaatc caagcgatgt aaagatcgat gtcttccgtt cctcgggtgc
     9361 aggcggacag aatgtacaaa agaacgctac tgccgtgcgc ctgacccatt taccgacggg
     9421 catcgtggtc acttgtcaga acgaacgctc gcaatcgcag aaccgcgaat ttgcgatgaa
     9481 gattctgcgc gcccgcctgc tcgaattgaa aaccgccgaa aaagaggaag agcgtgccat
     9541 tttgcgcggc gagtacacca aggctgagtg gggaagccag attcgttcgt atgtgttgca
     9601 tccataccag atggtaaaag accaccgtac cgaccatgaa acaggtaatt cccaggctgt
     9661 tttggatggc gacctgaacg gttttatgga ggcctttctg aaaagctcct tccccgaagg
     9721 gtaaagcgat gctgccaaaa ttgctccgga agatcttctc ccgtgaaaat tggcttgccc
     9781 tccttttgtg cctgatcctg atcgcgttga ccattttctc agcagacgcg actcctacgt
     9841 ggatttatca gggtttctag atgtctgtat ttagtgtcgg cgtgttggct ttcgccgcat
     9901 tcattgtagg gcgggttggg cgagggcgtg aactgacctt gttggcagcc agtgtttttt
     9961 ttatctactg gcttcagcct gcccaaacca tttccagtct ttcgttctgg ctgccgacga
    10021 ttaccctggc cctgtccatc ttttgctggg tgctggttgc gccgcctgag attcgtgttt
    10081 gggggaaaac ctggcctgcg gtgctggtga tcgtagggac aattctgctt gttgacttga
    10141 gtcaattcct tggttcgaaa attttttccg ccacgccgcg tttgtggatc gttgtgctgg
    10201 cgctggccgc tttttttgcg ctgacgtttt tcactgagaa acttccgcgc aggccgattt
    10261 tttttagcgc agttttgtgg ggaatagttc tgttgttcat atttctgaaa acgccttatt
    10321 ttttgaactc gttcttaaat tcgattttta caccatccaa ggaaggtgta ttattttcgt
    10381 ggcttggttt ttcatacgtc gcttttcgac tattgcacac cctgcgtgat cgtcaaacag
    10441 agcgcctgcc tgccctgact ctggcagaat acctgaatta cgttattttt ttcccatcct
    10501 tcgcggcagg tcccattgac agggctgagc gattcgtgct tgaactaagg aatcccgtcg
    10561 ctttagccga acaggattgg gtacaggcag ggacgcgatt ttttatcggc ctgttcaaaa
    10621 aatttgtcat agccgatgcg ttggcgctgg tctcttttaa cgacgccctc gcagaacaag
    10681 tccgctctcc ggtgtggatc tggtttcttt tgtatgcata ttccctgcgg atctattttg
    10741 atttcagcgg ttacaccgat attgccatcg gccttgcacg cattctgggg attcgtttac
    10801 ccgagaattt tgcggcgcct tttttgaaac ccaacctcac ccagttttgg aactcatggc
    10861 acatgaccct gacgcattgg ttccgtgcct actttttcaa tcccatcacg cgttcgttgc
    10921 gcacgaaaca actccctgtg ccgttgatta ttttcactgc tcaactttca acgatggtgg
    10981 tgatcggttt atggcatggc atcacctgga gttttttctt ttgggggatc tggcatggcc
    11041 tcggcctttt tattcagaat cgctggagcg aatggattca atcaaggatg gaaaccccgc
    11101 ctcctcctca ccgacaactg gtactgaatg tgatcggcat gttcctcact tttaattttg
    11161 tctcgctggg ctggttgttc tttacgctct cttcaccgct gcttgctttg caagtgttga
    11221 tgaaattgtt tggagccaca atatgaaagt gaatccctta cgtgtgcttg tcaaagcgct
    11281 gattttgtat gtggttgcga atattttgtt tgcaatctat aacccgccga tcggaaggat
    11341 ttctgtttat aactggcttg tgcctggtcg gcttcgtttt ccatatgaac gggctgagaa
    11401 cgtcaacctt ggctataaca tttccgtctt tcaagacctt gatgcggctt ttgcctctca
    11461 tgtcattgcg gatggagaaa aaccagcgga tgaataccgt gtgatcctgg tgggtgattc
    11521 ctcgatttgg ggtttcgcct caaattccac cgaaacgctc tctgcaaagc tcaacgacct
    11581 tgaattaacc gcctgcgatg gcaggcggat gcgtttttat aaccttggat acccttggtc
    11641 tttcatattt aaagacctgc ttgtacttga acatgctcgg gtttatgagc ctgacatggt
    11701 gttgtggctt ttcaccatgc gaggcttgac aggctcagat aaagatatag tcgacttctt
    11761 tgaaggtgaa ttgatcgttg atgccaagcg ggttcaggca aagtacggcc ttgaagatta
    11821 tacgagtgag ttgaccactc aaaccttttt cgagcaaacg atcatcggag agcggaagaa
    11881 aataaaaaca gatatcaccc tgcaaatgta cggctttttg tgggccgcca ctgggattga
    11941 ccatcacctg cagacctggc ttccatggaa agatacgttg gagaacagca ttgaatatcg
    12001 gcattatcga acccccgaag aatggccgca attgcaggac gatttcatgc tcgatgtgct
    12061 cgatgctgcg cacacaatga tgggtgatac gaagatcgta gctgtaaatg agcctatcta
    12121 catttcaagc ggatcgaaca gcgaggtccg ctacaacaag ctgtatccgc gttgggcgta
    12181 tgatcggtat cgggaattta tgcagggatg gatggcggaa aatcaatatc cgtatttgga
    12241 tatgtggaat gtcattccgc aggaagagtt ttcagatttc cccgttcatt tgtatcccac
    12301 aggggagcgg ctgcttgcag aatcccttgc ccctgaagtg ttgaaatttt cctgcccgta
    12361 ggtggatgaa tcaaaagagc gagtggcatc actcgctctt tgggtttatt agttgctgga
    12421 agcccgtttt tcgagagttc ggttgaacag ctcttccttt tcgtctgaag cagacttata
    12481 ggatttggtg cggactttgc cgcgaccctt tgagcgttca agcgcctgct gccaggcgat
    12541 ctgcatggca gtgaattgag gctcgttgga aacttccact tccatttcgt aagcttcttc
    12601 ttcctgcttc ttgcctttct tgcctttggg tttgtcaccc ttcttgctgc gttcttcgcg
    12661 ctcgggcttt tcttcctcaa cgatttcggg gagaagggcc ttcatgctca gacggatctg
    12721 gcgcttgcgg cgatctacat ccaaaacctt cgcttcaact tcatcgcctt ctttgacaac
    12781 ttcgccagcg gtctttacat aaccgtgtgt cagttcgctc acgtggatga gaccagggcg
    12841 ttcagcgcca aagtcaacaa atgcgccata agtttcaatg cggacgactt tgcctttaac
    12901 ggtcatgtcg ggtacgatct ctttccattc gtaaccgagc ggctcgatca tggtcagttc
    12961 aatgcggtcc ttcttgacgc ggcggaccca aacctcaaca gtttgtcctt cctttaccac
    13021 atcttccact ttgtttactg catcctgctg aagttgtgaa atgtggatta caccgggaag
    13081 actttgtccg atgtccacaa gcgcccccgc gagagttgtc ttcaaaattt tgccgctaag
    13141 tttggttttt ggttcaagcg ctacggcggg tgcgctgtcg ggtgttgtca tagttatact
    13201 cctactattt gaaataatgc cgaaaaaaga ttatacctgt ccgacaacaa atcgtcaata
    13261 agaagttggg agaagttact tcattgatat ctgggtcttg ccgattggtt gtgacctgtg
    13321 tataatccga aaaatcaaat gcgttgataa gaacagtaga ttcaccagaa atgttgtcag
    13381 cgagcctgtg gttggtgtga gacaggtcaa cagggtagat cgaagatcct cttggagcat
    13441 caaactgaaa gcagaaaaga agcggatggt tttcatccaa agcttggctg ggagtaaggg
    13501 agacggaaac cgccgttata cgggaacaac ctcagtgatt gtgtttgctg gcgttgaatg
    13561 agcgcctgct ttgacagcag gaacttgggt ggtaccgcga gattctctcg tcccaatatg
    13621 tggacgggag tttttgttta aaagaatttg gccgatggcc ggtcaggaga tgattatgtt
    13681 taaagctgta aacccaaaac ttaatgtgcc attgatggaa gagaccatcc tcaagctgtg
    13741 gaagaagcag aatgtgttca agaagactgt tgagcagcgc aaggacggcc ccgaatatgt
    13801 tttctatgaa ggcccaccaa cggcgaatgg acgtcccggc gtacatcatg tgttggcgcg
    13861 tgctttcaag gatatgttcc ctcgttacaa gatcatgcgt gggtaccacg tttcacgccg
    13921 cgggggatgg gacacgcatg gcttgcctgt tgagatcgaa gttgaaaagc aacttgggtt
    13981 caccaacaag cagcagatcg aggaatacgg catagataaa ttcaatgaac tgtgccgaaa
    14041 ctcggttttt acctacattc aggattggga gaagctcacc gaccgcatcg cgttttgggt
    14101 ggacctcgaa actgcgtatg tgacctatga aaatgaatac atcgaatcag tgtggtggat
    14161 cctgaaaaac ttctgggaaa aggacctgct gttcaagggg tacaagatcg ttccgtattg
    14221 cccgcgttgc ggcacgcctc tttctgacca tgaagttgct cttgggtacg atgatgcggt
    14281 ggacccgtcg gtgtttgtcc gcatgccgct cgtggataag ccagatactt ccctgttggt
    14341 gtggacaacc acaccgtgga ctctccctgc caatgtggcg gttgctgcac atccagaagt
    14401 ggattatgtc accatcgagc gtgacaacga tggcacgaag gaaaagctca tccttgcgaa
    14461 gaatttggtt gaaaagatct tcgcgggtga agaagtgaag gtagtggact ccttcaaggg
    14521 taaaaagctg aagggtttga aatacaaacc actctttact tttgtgcccg tggataaacc
    14581 cgcctatttc gtggtgctgg gtgattttgt cacgaccgaa gatggttcag ggcttgtaca
    14641 tcaggctcct gctttcggtg cagaagacat ggaaatggcg aaacagcatg acctgcccat
    14701 tttgctcacg gtgcagcctg acggcacctt tatccctgag atcaccccat ggcgcggaat
    14761 cttcgtcaaa gacgccgacc cgcagatcat ccaggacctc gatgcgcgtg ggcttttatt
    14821 ccgcgcgggc acgctcacgc atacctatcc cttctgttgg cgttgtaaga ctccgttact
    14881 atattatgca cgcgagtcat ggtacatccg caccagcgca aaacgggatc ggctcgtgga
    14941 actgaacaag acaatcaact gggtgccaga tcacatcaaa aacggacgat tcggtaactg
    15001 gttggagaac aatatcgact ggtcgctcag ccgcgaacgg tattggggca cgccgctccc
    15061 tgtttgggag tgtgaaaatc aggaatgtaa acaccgtgag tgcgttggtt cggtggctga
    15121 actttctgaa aaagctggca aagacctgac tgaactcgac ctgcatcgcc cacatgtgga
    15181 taatgtccac tggaagtgcg tggagtgcgg cgggaagatg tcgcgtgtga aagacctgat
    15241 cgatgtgtgg ttcgactcgg gatccatgcc ctatgcccag tggcattacc cgtttgaaaa
    15301 tcaggataag ttcaaacagc aattcccagc ggattacatc tgcgaagccg tggaccagac
    15361 ccgtggttgg ttctattcgc tgcatgccat cagcaccttg ttgaatgacg atgtgtcgtt
    15421 caagaatgtg atctgccttg ggctgatttt agatggagag ggacgtaaga tgtccaaatc
    15481 cattggcaat atcgtcaatc catgggatgt gttgaatgtt cacggggcag acgcatttcg
    15541 ctggtatttg tacaccgcct cgcctgcggg gcaggagcgt cgcttctctt ccgatttggt
    15601 cggggacgtg atccgcagtt tcacacttac gttgtggaat gtgtattcgt tcttcgtcac
    15661 ttatgccaac cttgataagc cacaggcatt gaccatcact gccccgacga acgatctgga
    15721 ccgctggctg ctctctgagt tgaatgtgct cgtccgtgat gtgacgaatg cctatgaaaa
    15781 ctatgatgcg accaatgcca cccgccccgt ggaaaaattt gttgaaaccc tttccacgtg
    15841 gtatgtacgc cgctcgcgcc gccgcttctg gaagaatgat tcggattcgg acaagcaggc
    15901 cgcctattcc acgctttaca ctgcgttggt gacagttgcg aagttgatcg cgcctgccat
    15961 gccttttgtg gctgaagagt tgtaccaaaa ccttgtccgc tctgtggatg agacagcgcc
    16021 tgaatcggtg catttggcag aatggccgaa ggtgatggaa gaattcattg acgagtcgct
    16081 gaaccgcgat atgaatctgg tgatgaagct ggtgtcgctt ggacatgccg cccgtcagaa
    16141 ggcgaaccgc aaagtgcgtc agccgttggc cgaagctgcc ttctcggttg ggaacaatgt
    16201 cgaacgtaat gcgctttctg ccaacatgga tgtgatcgcg gatgaattga atgtaaaaca
    16261 ggtgcgcctg ctcgatgcct ccaccgaagc gttgacgcac accgtaaaac cgctgcccaa
    16321 gcagttggga cagaaatatg gcaacaagtt ccccgccctg caaaaggcga tccttgccat
    16381 gaatgccgag gaagttgcga agatcctgca ttcgggtgcg ccgttgaatg tgaccgtgga
    16441 cggtgaaacc tatggcatcc tttccgatga agtggaagtg aaggcattgg cgaaggaagg
    16501 atttgccgtg gctgaagacg gtgcctatgt cgccgcgctc gtcactgacc tgacgcctga
    16561 actcattgcc gaagggcagg cacgtgaatt tgtccgccgc gtgcaggacc tgcgcaagac
    16621 cgccgacctg gacgttgccg atcgtgtgga actgttcatc gaagccacag caggtttgag
    16681 gtccgcgatt gaagcgcacg agggttacat caaagccgaa acgctgacgg tcaaactgtc
    16741 ctttgcgaat ccgcccgcca ccgcatctgt tgttgaagat gaattcgatg gcgagaaggt
    16801 gaaggttggg ctggtgaaga gtaagtgagt tgggaatggg ggatagggaa tagggagtag
    16861 tgaatagtga atagggaata gggaagccct cggaggtcat gagacttccg agggctttta
    16921 taaaatttcc atttcaagtt ctcccccaaa ttcgacagca ctatcgtcgt atttggggga
    16981 gatgtccgaa ggacagaggg ggtgcgcctt atgcaggcaa attattgatc agtttgataa
    17041 ccgtcattgc aagttccagc acgggcttgc cgatctcatt taaattcttt gcagcttggg
    17101 ccagcccttc cacagtgacc ttgatccgtt cctttttggg tttggatttt ttcgcttcgg
    17161 tttgcaggct ttccagttcg tctttgatct cttcggtgtg ttcggattcc tcggcttttt
    17221 gcaggagcgt ttcggatgcc ttcatcaact ctgtcagagt gttttgcaat tcggcaggga
    17281 aggtgttgaa gctgttttgc acgctgacag tggatgtgtt gccgtcaccc aaagttaacg
    17341 aactgccgtg catgtttccc attgtgatgt tgttgttcgt gatgtaggtg tcgccgcgct
    17401 tgccttcctg cttttcgcgc tcttcatctt tgcggcgttc ttggggagtg acgaaaccat
    17461 cgagcaattc gcgcaaatta atggtaacgg gtttgtcgcc gtccaattca ggtatttgag
    17521 tcgtgcctgt cttgagtaga tttttcatct tgtcaaaacg caactgcaca ttggggtatt
    17581 gcggcgggta gatgaaggct tcgggcggag tggcaaacat ggtgtggatg tcgtcgaagt
    17641 gggcgcggag aatggcaagc gcgtcgcggc gcgttgattc ttccccagag atgaaaatgc
    17701 ggatgcattt gtcctcttca tcggcttgga caagagccgt gtttccatcc agccttaaga
    17761 ccacgccgct gcgccagact ttgttatcca gaatccgtgc gtgggttttg acaatgaagc
    17821 ggacgatgac gctcttgggc agttcggggt agccatattc aaacttcatc gcggacgatt
    17881 tcaaattgcc gaggtcttcg tctatttctt ttgccatcag gctggggagc cagtaggagg
    17941 aatcatccgc gcggtagcac aattcgaagc gttcgagcag tttcgcgatg tcatcaatgt
    18001 ggcgggaata ttcgggcgcg gacaaaatct cgcgcagata ggccttgctc aatttgcctt
    18061 tgtatttgtc cttcaacgcg gggttggtga caatgagata cacgccgcgc gtgacccagt
    18121 tggggttgag cacgccgagc gcgggcagca gcggattccc gtccgcgtcg cggaagtaca
    18181 agaccgtgcc gaggtcgtgc agcaggtcga gcagggtgtc ctggtctttt gcgtcgctga
    18241 cgccgtgccg ctcacagacg cggacaaatt cttcgtggga gatgtgcgcc gcgctgaggg
    18301 tttccatctc gtctttgacg gcgagataat ccgcgctgaa gaggacgcgc acctgcggca
    18361 tggcgcggac ttcctcctga attttttggc gcagttcggc gatatgttcg cctgtgctgg
    18421 cggacgtgcc gacgaagccg cgaatgttgg gatacttttc gcgcaggcgg cgtttttcga
    18481 cgtccatttc gtgctggtcg catttgttgc cgaccaaaat gacgggcgcg ttttggctgt
    18541 aggcgttgat gagcgtcagc caatattcaa tgttattttg ttcgagcgtt tttcccgtat
    18601 cgaagaccag caggtacaaa ctgcgccccg agaaaaagaa ttg
//
LOCUS       DAOQLC010000085        18481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1047_length_18481_cov_24.040975, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000085 DAOQLC010000000
VERSION     DAOQLC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1047_length_18481_cov_24.040975"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..120)
                     /locus_tag="PL000_14370"
     tRNA            complement(34..120)
                     /locus_tag="PL000_14370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(84..86),aa:Ser,seq:cga)
     gene            complement(189..1583)
                     /locus_tag="PL000_14375"
     CDS             complement(189..1583)
                     /locus_tag="PL000_14375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC90137.1"
                     /translation="MRISFHGAAHTVTGSQHLIEVNGSRLLLDCGLYQGKRADTYERN
                     LNFSHDPRKVDAVILSHAHIDHAGNLPNLVKQGFEGPIYATRATVDLASLMISDSGHI
                     QESDAEFVNKKRAQRGEEPIEPLYTKEDAEKVAGMFSGVNYGEAFEPIPGVVARFYEA
                     GHILGSAGVSLDIEEKGKKTRLWFSGDIGRYKLPLLRDPVLPDRADYMIMESTYGDKS
                     HNDPSKAFIEFRDVVKRTIERGGKVIVPAFAVGRTQELVYHLNEMMTEREIPSVPVFV
                     DSPLAVNATSVFRNHPECYDEETRQFVLEARHPALDFKMLTYVKTVDESKALNERTDP
                     MVIISASGMAETGRIVHHIRNNIENPKNTICIVSWQAPDTMGRRLADREPIIRIFGEA
                     YKRKCDVATIGGLSGHAGQDLLMKYALNVKDSVKQIFLVHGEEKQAMTLKGLLKEQKL
                     NQVHYPDLHESVEL"
     gene            complement(1573..2709)
                     /locus_tag="PL000_14380"
     CDS             complement(1573..2709)
                     /locus_tag="PL000_14380"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNC90138.1"
                     /translation="MPNVLPPPVWINSKPLLQKMMDDLVTQKRVAVDTESNSLHAFRE
                     QVCLIQISSSNEDYLIDPLELRDLKLLAPIFSDPKIEKVFHAAEYDLICLRRDFGFSF
                     ANLFDTMHAARVLGYPAVGLDRLLGDKFNIQMDKRHQKANWANRPLTREQIHYARLDT
                     HYLFDLRDALELELREKARWDFAKDDFTRACLPEEIKLRTNGESWERFSGRKDLSLRE
                     LTIMAHLCKWREKEAEKLNRPLYKVIMDDTLITLSKNQAQQKVDLSAAGLSEKQIHLW
                     GEAVLGAIRDGVSAPLVERKQPEVKNDAFLRRLEKLKVWRKNVGQEMGVESDVILPKV
                     YLSALAENPPNTADELAHLLKGTPSRVEKYGPQILKLLGVKNAN"
     gene            complement(2748..3764)
                     /locus_tag="PL000_14385"
     CDS             complement(2748..3764)
                     /locus_tag="PL000_14385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC90139.1"
                     /translation="MTVGKQRLQEFEALRALSIVLLLILHSDVFSLSVFGFELGPISH
                     FVGAFLLGSFFFLAGYFQEVSMQKRGYEPLAYVKSKFLRIFPPYWFALALFIFVMGYS
                     LKRFDLTVYLLNIQFIFSPTFVKQMLTLWYISVVVGYYVLFGLLANRLRSNSGLMIWS
                     AVIFAATYVLHKMTGLLDGRFFEYFFIFLAGVYFARFQQIRERLFSLHFAYKVGVAFL
                     GVLAFRMALTSGVDITHPLYLLAVDFYILGWVWMWLTIFRGGAGAWRIWAPISTASFF
                     AYLFHRPIWYILVGLLAKRMGISTIMFQFIPGSIAVLVICYFLQNGYDTLLRMGRKST
                     RAEI"
     gene            complement(3761..4192)
                     /locus_tag="PL000_14390"
     CDS             complement(3761..4192)
                     /locus_tag="PL000_14390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90140.1"
                     /translation="MQIIPATLFDLNSLRKLEKACFEKDAWPLFDLIAVLTFPEVVRL
                     KAVVDGQMAGFVGGDPRPHEGWGWIATIGVDPRFQRRGIGRALLHACEAKLGVPRSRL
                     TVRMSNYAAISLYEQEGYVTTDIWSRYYNDGEDGLVMEKNL"
     gene            complement(4277..5185)
                     /locus_tag="PL000_14395"
     CDS             complement(4277..5185)
                     /locus_tag="PL000_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC90141.1"
                     /translation="MNGVLGSVGALFLCLGLAVVVFLAFNFLRIVPEYQRLVVFRLGR
                     VLDKPKGPGIVLLIPGADRPVKVDLREQVREVPHQTSITKDNAPISIDFLWYYKVLDP
                     AQSVLQVGNFESAAAGIAATTLRAVIGGIPLDDVLSEREHINNMLRTRLDEVTERWGV
                     KVTNVEIREIIPPRDVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQSNILR
                     AEGEKQSAILKAEGEREAQLLRAEGFAKALEQIYNSAKTVDQKTMTLQYFETLKAMGM
                     SPSTKYIFPMEFTSMLDNFLGKDTKK"
     gene            complement(5210..5803)
                     /locus_tag="PL000_14400"
     CDS             complement(5210..5803)
                     /locus_tag="PL000_14400"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNC90142.1"
                     /translation="MDFLLDPNVAYLIFLTGIVLVFIAVATPGTGLVEIGALFCFILT
                     GYALYNLSVNWWALVILLLSVVPFIQAIRKPKSFLFLALSILLIVIGSVFLFASDDGS
                     LISVNPVIALISSGLVAVFLWFLFRKAIEAISRRPTHDLETLVGQIGEAKSAIYGDGS
                     VYIAGEMWSARSDSEIPAGSHVRVIRRDGFTLVVEKQ"
     gene            5941..7257
                     /locus_tag="PL000_14405"
     CDS             5941..7257
                     /locus_tag="PL000_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC90143.1"
                     /translation="MKFQKICVIGLGYIGLPTATTFAAHGIEVTGVDINQNIIETLKK
                     GEIHIHEPGLRDGFVSAVQSGRFKAATSPEEADAFIIAVPTPFQENEYGSHNGMTYKL
                     ADMRAVISAAESIVPFLRKGNLVVLESTSPPRTTLDLVAPILARSGLEAGRDFHLAYS
                     PERVLPGQILRELIENARVIGGITPESAEAGYELYSTFVKGKIIKTDATTAEMVKLME
                     NTYRDVNIAIANEFSRLANKFGVDVWEAISLANLHPRVKILNPGPGVGGHCISVDPWF
                     LVEAAPELTPLIYHSRRVNDTQPHFVVETVKRALGSLNGKRIAALGLAYKPDVDDLRE
                     SPAAEVVHLLQHEGAQVKAWEPFKPNANMNGIHMSPNLEDAIQDADTILLLVRHTEFK
                     QISPREIAGKTSAKIIVDTVNSWEGEAWKSSGFEFFRIGDSKSPNS"
     gene            7295..8401
                     /gene="wecB"
                     /locus_tag="PL000_14410"
     CDS             7295..8401
                     /gene="wecB"
                     /locus_tag="PL000_14410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC90144.1"
                     /translation="MKILSVFGTRPEAVKMAPVVKLLEKTAGIESKVCVTAQHRQMLD
                     QVLNLFEIQPDYDLDLMRDNQSLAQLSANIFTHLDPVLDDFKPDWVLAQGDTTTVAIT
                     SLLSYFRRIKFGHVEAGLRTHDKWQPFPEEVNRRIAGVTADMHFAPTEWSRNNLLREG
                     VEESLIKVTGNTVIDALQFVSKQPEPEEIRQLVKEKLASSRLILVTAHRRENFGLPME
                     GICHALKELAQEEGVEIVYPVHLNPNVQEPVNRILKGVDHITLLPPLDYLPLVHLMKH
                     ATIILTDSGGIQEEAPAFGVPTLVLRDVTERPEGVQAGTLKLVGTETSHIVQEAKRLL
                     YDKSAHAQMAKAANPYGDGHAAEKIIHALLENGQ"
     gene            8524..9465
                     /locus_tag="PL000_14415"
     CDS             8524..9465
                     /locus_tag="PL000_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90145.1"
                     /translation="MQRRYLTLFLVIFMLSTTWVPAHAQDQGSKYFSETGHNVKGDFL
                     VFYNSNPNALFLYGYPITEEFIDKNNKTVQYFQRARFEYDPNQPAGQRVQLTQLGRQT
                     YISTGKLDVANTLACRTYSETGFAVCFAFLEFFDQYGGLTQFGYPISGFEYHENKIVQ
                     YFEKARLEWQPWKTEGQRVVVSDLGRIYFDALGEDPGLLSPVKPLNNTNQDVISLQVR
                     AFVWKAVTLATDSQLVFVIVQNQNLQPIANATCSTIVNWPDGRSENMSLTTNTNGVGI
                     VPLSFVNQPYGSLIYTDVSCSYGNLSGKTTTSFRIWY"
     gene            complement(9552..10151)
                     /locus_tag="PL000_14420"
     CDS             complement(9552..10151)
                     /locus_tag="PL000_14420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC90146.1"
                     /translation="MPWSKRIFDFIFALIALIIFSPVLIVTAILVRVFIGAPILFTQQ
                     RPGYKGRPFNIYKFRSMTNRIARDGSLLPDAERLTRFGRILRSLSLDELPELFNILRG
                     EMSFVGPRPLLMEYLPLYSPEQMRRHDAMPGLTGWAQVNGRNALDWPSRFRLDVWYVD
                     HWSFWLDLRIILMTVGKVIAREGVNQAGQSTVEYFKGEQ"
     gene            10219..10896
                     /locus_tag="PL000_14425"
     CDS             10219..10896
                     /locus_tag="PL000_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90147.1"
                     /translation="MNERILIIEDDQAILKLLQRGLAYEGYTVDTATDGRMGLIAARD
                     HTPDLVILDWMLPGMDGLEVCNRLRTGGSIPILMLTAKDTVQDRIQGLDAGADDYMVK
                     PFNLDELLARVRALLRRTQPERIPVLKFADLSLDTGSRQAARGSRIVALTAKEYELLE
                     LFLRHPKQVLTREVIFDRVWGYDFGGESNVLEVYIRYLRQKLEGEGEARLIHTVRGVG
                     YVLRENP"
     gene            10984..12351
                     /locus_tag="PL000_14430"
     CDS             10984..12351
                     /locus_tag="PL000_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90148.1"
                     /translation="MSLRLRLTLLYSTFMSGILLIFGAAVYILVNVILLNQVDTMLAG
                     VVRDITQVTKVNPMGELNLISLPPLDMTSNAYVQVWGPDNKIIATSPSIGTLTKPLDP
                     IGLESDMVVYKDSYLSGAHLRVLSVPLKVGDRFIGTLQVGASLSVVDATQGNLLSMMA
                     AIAVVAVLLAGFGSWVVLGRALSPLEAITETVDQINRADDLSRRIPSQERSEDEIGEL
                     ITSFNQTLERLESLFTSQQRFLADVSHELRTPLTVIKGNVDLMRHFKQVDEESLTSID
                     QEAGRLTRLVGGLLMLAQAESGKLTLVMKPVELDLLLTEVFTEMCVLAGSKVHVHLNE
                     IDQVMVNGDRDRLKQVLLNLVANAIQYTPQGGEVFLSMKKIGDQARLIIRDTGPGIPA
                     EDLPHIFDRFYRAEKSRTRSTSSGFGLGLSIAQWIVENHGGQIKVESKEGKGTTFVIW
                     LGIVK"
     gene            complement(12398..13498)
                     /locus_tag="PL000_14435"
     CDS             complement(12398..13498)
                     /locus_tag="PL000_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNC90149.1"
                     /translation="MALTIGTAAAKRGGITYGEYALVQHPVGGADSLPVIIAQGEEEG
                     PVFWVTAGIHGIEHAGIQVIHKLITPELVGQMRGTIIAIPALNPAGLRTLNRQAYYHA
                     GDPNRLFPDGRKPKTNDPDQNLPSALEQAYGRLFEEIKNTASFYFDLHCMSNGALSFT
                     FRDRILYRKGDEAARQRAEVVSTKIDEMAQAYGHTVIADFAVEKYIDQKLHRATTAAV
                     SMLAGIPALTAELSSPYTPTPEIVHAGVAGLRNVMRWGGMLGGEMEPILGIKVVDPGF
                     PTHRRSVARVTQSCIVHHLKEPGDLVKVGDPLVEMRDAWGRSLGVLNSEYEGFVISRP
                     TGILYYPGEATMLLAIRDEKPRIGEYPETYFD"
     gene            13550..14383
                     /locus_tag="PL000_14440"
     CDS             13550..14383
                     /locus_tag="PL000_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90150.1"
                     /translation="MITALNFPSFDEHFSDLNNFILYLVQQYQTYRLASWEVLAHRCR
                     QFYTPDVMDAIEAKAPGWKKMASYSEGITLEHVTCVFLGMYMLPEFQELSAEQQQMAK
                     WIILFHDIDKAHIRGQRDTMHAFHSAIVAARDLPGLGFPVTDKYASLIDAWSEYTRQA
                     FVQPADQNWPKPDNQKLPNILSGIEDMFGEQAPATLIIKTALLHISLLVDRNYQTPAP
                     LTDKEVKRFISPALFPLLRVMMFADNEGWSLFDLEFRDQQKHDSSIEFERIRKFISPT
                     S"
     gene            14380..15684
                     /locus_tag="PL000_14445"
     CDS             14380..15684
                     /locus_tag="PL000_14445"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90151.1"
                     /translation="MTNKSSHDPYAALRLRDFRLLLIGRFITSFGNEMLSFAIGWELW
                     LRTRSAFALGMVGLVQVVPVILLSLPGGHVADQYNRRRIIVITQAVFSLCSAGLALLS
                     FFDGPLVLIYLCLLGFGIARAFNEPASSTLIPETVPSELFASAATWNSSTWQTAAISG
                     PAIAGLVAGFLNSVTIIYVFDAIAALVFLFLVTQIHGKPLPLSEKSDTMASLMEGFRF
                     MRDTKIILAAITLDMFAVLFGGAVALLPIYATDILKVGAQGLGIMRAASSIGALMMAF
                     GLAHLPPFKQAGKTLLWAVAGFGAATIVFGLSRSFWLSVAMLALLGALDNISVVIRST
                     LLLTRTPNEMRGRVSAVNSIFISTSNEMGAFESGLAASLFGPILAVVGGGIGTIIVVL
                     IVARAWPEMRNLKSLADLEEPAPAQFTSPNQGDENAPSSTHP"
     gene            15659..16189
                     /locus_tag="PL000_14450"
     CDS             15659..16189
                     /locus_tag="PL000_14450"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC90152.1"
                     /translation="MPLQAPTPDEYASFYSDYIQRAVARGDVLAAMPTQIDELDSALG
                     NLTDKQARFRTGPEEWSIKEIVSHLIDAERVFSYRLLRVSRNDTTPLPGFEQNGYVST
                     SGADDLPLGDLLAEFSFLRRANILAIGHMSTDAVKRRGTVSGATVSANALIHMLVGHV
                     DHHMASLHEKYLPNLH"
     gene            16307..17176
                     /locus_tag="PL000_14455"
     CDS             16307..17176
                     /locus_tag="PL000_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90153.1"
                     /translation="MANYQRNLGDGLKTIWNNFIRNRKFNRGAAWGMMIIGALLSFEI
                     FNFSTTQFALRDMLGDLTFAGFRWATILAIAFCGIDFAGIARIFTPEQGRDEPAEVYY
                     LFGAWLLAAGFNATLTWWGVSVAVAENGNVHAAGALSAETLTKAVPIFVAVMVWLVRL
                     LIIGTFSLAGDRLFTTADQPSAQHRPQYQSQPQTQRAYNNPQPQQRPAYQNSQPQRPS
                     YNQPVNQPILRPASQIARPANSPSTTNFRPAPKPTVNSTQTSFIPPEPTYHPISYEAR
                     TNSVESSTRHYDA"
     gene            17295..17984
                     /locus_tag="PL000_14460"
     CDS             17295..17984
                     /locus_tag="PL000_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC90154.1"
                     /translation="MPRARTSQIDRQTAAPQQEQGSGCFSGFMLVPLSVIFISTLLAA
                     LAMKTTPIPSSRVDLPPSTSTGIALIFTPEIQYWAEDIAQWAAASQLDPNLVATIMQI
                     ESCGDPRAISSAGAMGLFQVMPFHFAASDSPYDPDTNAARGLAYLVRSLQAGGGDARL
                     ALAGYNGGIGILSRAEWTWPAQTKRYIQYGVPIYQDARNGLHHSEAVTEWHETYGASL
                     CRQAHNRLGLP"
     gene            18096..>18481
                     /locus_tag="PL000_14465"
     CDS             18096..>18481
                     /locus_tag="PL000_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90155.1"
                     /translation="MKISNTLYVTAPKDWRTWLKQHYKTEAEVWLVYPKKATGKPRIE
                     YNDAVEQALCFGWIDSIIKTLDDEHTVQRFSPRKPKAKYSQANIERLRKLVAQKKVIK
                     EVADTLTDILNAEFIIPPDILKAIQA"
BASE COUNT         4627 a         5032 c         4462 g         4360 t
ORIGIN      
        1 aaaaaagccc aaccgatttg gctggacttt tagcggagag ggagggattc gaaccctcgg
       61 tggaccggag cccacaacag ttttcgagac tgtcccattc aaccactctg gcacctcccc
      121 atttctcgaa gcgggcgaaa ttataaccga aaaaaatacg cccaatccgt tcaatatatg
      181 gagtgtgttt acaactccac actctcatgt aaatcggggt agtgaacctg attcagcttc
      241 tgttccttca aaagcccctt caaggtcatc gcctgcttct cttcgccgtg gaccaaaaat
      301 atctgcttga cgctgtcttt cacattcaaa gcgtacttca tcaacagatc ctgccctgca
      361 tggcctgaga ggccgccgat ggtggcaaca tcacatttac gtttataggc ttcaccaaaa
      421 atgcggatga tcggttcacg gtctgcaagc ctgcggccca tcgtatccgg tgcctgccat
      481 gaaacaatgc agatcgtatt ctttgggttt tcgatattat tgcgaatatg atggacgata
      541 cgccctgttt ccgccatgcc cgaagcggaa atgatcacca tcggatcggt acgttcattc
      601 aacgccttgg attcatccac agtttttaca taagtcagca tcttgaaatc cagggctggg
      661 tgacgtgcct ccaacacaaa ctgacgggtt tcttcatcgt aacattcagg gtgattgcga
      721 aaaaccgaag tggcattcac agccagcgga ctatccacaa acaccggcac actgggaatc
      781 tcgcgttcgg tcatcatctc attcaagtga taaaccaatt cctgcgtacg ccccactgca
      841 aaagcgggca cgatcacctt gccgccgcgc tcgatcgtcc gcttgacgac atcgcgaaat
      901 tctataaaag ccttcgacgg gtcgttgtga cttttatcgc cataggtcga ttccatgatc
      961 atgtaatcgg cacggtctgg caaaacaggg tcgcgcagta atggcagttt atatctgccg
     1021 atatcaccag aaaaccacaa gcgggtcttc tttccctttt cttcaatatc cagcgaaacg
     1081 cccgccgaac cgaggatatg ccccgcttca taaaaacggg cgaccacgcc agggatcggt
     1141 tcaaacgcct ccccgtaatt cacaccgctg aacatgcctg caactttctc agcatcttct
     1201 tttgtataaa gcggctcaat gggttcctca ccacgctggg cccgcttttt gttcacgaat
     1261 tccgcatctg attcttgaat gtgtccagaa tcggagatca tcaggctggc cagatccact
     1321 gtagcgcggg tggcatagat cggcccttcg aagccctgct tcacaaggtt gggcaggttc
     1381 ccggcatggt caatgtgggc atgagacagg atcaccgcat ccaccttgcg cggatcgtgg
     1441 ctgaaattca aatttcgttc gtaggtatcg gccctttttc cctgatacag gccacagtcc
     1501 agaagaagac gcgaaccgtt gacttcgatc aaatgctggc tgccggtcac agtatgggcg
     1561 gcgccgtgga aactaattcg catttttgac tcctaataac tttaatatct gcgggccata
     1621 tttttccaca cgtgaaggag tacccttcaa caagtgagcc aattcatcag cagtgttagg
     1681 cgggttctca gccagcgctg ataaataaac ctttggcaga atgacatctg actcgacacc
     1741 catttcctgc ccgacattct ttctccacac cttcagcttt tccaaccgac ggagaaacgc
     1801 atcattctta acctcaggct gtttccgttc aaccagcgga gccgacaccc catcacggat
     1861 ggcccctaaa accgcctccc cccacaagtg gatctgtttt tcactcaagc ccgccgcaga
     1921 aagatccact ttttgttggg cctgattttt cgacagggtg atgagggtgt catccatgat
     1981 caccttgtaa agcggacgat ttaatttttc agcttccttt tcacgccatt tgcacaaatg
     2041 cgccatgatg gtcaactcgc gcagcgacag atccttgcgc cctgaaaacc gctcccacga
     2101 ctctccgttt gttcttaact tgatctcttc cggcaggcag gcgcgggtga aatcatcctt
     2161 cgcaaaatcc caacgggctt tttcgcgcag ttccagttca agggcatcgc gcaggtcaaa
     2221 aagataatgt gtatccagcc gcgcataatg gatctgttct ctggtcagcg gacgattggc
     2281 ccaattggct ttttgatggc gcttgtccat ttggatattg aatttatcgc cgagcaaacg
     2341 gtcaagccca acggcggggt atcccagcac acgggcggca tgcatcgtat cgaaaagatt
     2401 cgcaaaggaa aaaccaaaat cacgccgcag acagatcagg tcatattcgg cggcatggaa
     2461 tactttttca atttttgggt cggagaagat cggcgccagc agtttcaagt ccctcaactc
     2521 aagcgggtcg atcaaataat cttcattgga tgaagaaatc tgtatcaggc aaacctgctc
     2581 gcgaaaggcg tgcaggctgt tcgattctgt atccaccgcc acacgtttct gtgtgacgag
     2641 gtcatccatc attttttgta ataggggttt tgaatttatc cagacgggcg gtggcaacac
     2701 attgggcata tcggccgatt ataaaccttc tacaggcagg cgaggaatca aatctcggcc
     2761 cgcgttgatt ttcggcccat ccgcaaaagg gtatcgtatc cgttttgcaa aaaatagcag
     2821 atgacgagga cagcgatcga gcctggaata aactgaaaca tgatggtgga tatccccatc
     2881 cgttttgcca gtaaaccaac caaaatgtac caaatggggc gatggaatag atatgcaaaa
     2941 aacgaagctg tggaaatcgg cgcccaaatt cgccatgccc ccgccccacc ccggaagatc
     3001 gtcagccaca tccataccca gccaaggatg taaaaatcca cagccagcag gtagagcgga
     3061 tgcgtgatat ccacacccga ggtcaatgcc atacgaaacg ccagcacgcc caaaaacgca
     3121 acgccaactt tgtaggcgaa gtgcaggcta aaaagccgct cacgaatctg ttgaaagcgt
     3181 gcaaaataaa cgcctgccaa aaagatgaaa aagtattcga aaaacctgcc atccagcagg
     3241 cctgtcattt tatgcaacac gtaggttgca gcaaagatca ccgccgacca gatcatcagt
     3301 ccgctattag agcggagacg atttgctaga agaccgaata aaacgtaata tcccaccact
     3361 acgctgatat accacaaggt caacatttgc tttacgaagg tcggcgagaa gatgaattga
     3421 atgttcagca ggtacaccgt caggtcgaag cgtttcagcg aataccccat cacaaaaata
     3481 aacagtgcca gcgcaaacca atagggtgga aaaatgcgca ggaatttgga tttcacatat
     3541 gccagcggtt catatccgcg tttttgcatg gagacttcct gaaaatatcc cgccagaaag
     3601 aaaaaggatc ccagtaaaaa agcgccgaca aaatgcgaga tcggtcccaa ttcaaaacca
     3661 aaaacgctca aagaaaacac gtcactatga aggatcaata acaggacaat ggaaagcgca
     3721 cgcaatgctt cgaattcctg taaacgctgt tttccaaccg tcataaattc ttctccataa
     3781 ccaacccatc ctcgccgtcg ttgtagtatc tgctccaaat atcggtggtg acgtagcctt
     3841 cctgttcata aagggaaatg gccgcataat tggacatgcg taccgtcaaa cgggaacgcg
     3901 ggacacccaa tttagcctcg caggcatgca acagggcccg cccaataccg cgccgctgaa
     3961 agcgtggatc caccccaatg gtggcgatcc agccccaccc ttcgtgtgga cgcgggtctc
     4021 ctcccacaaa tcctgccatt tggccatcca ccactgcttt gagtcggacg acttcaggaa
     4081 aggtcaacac cgcgatcaaa tcgaagagcg gccatgcatc tttttcaaag caggcttttt
     4141 ccagtttacg aagtgagttc aggtcgaaaa gtgtggctgg gatgatttgc atgacccgat
     4201 gattttatca ataaaaaagc aggaagcgga atatccgcct cctgcttttt acgtcattta
     4261 ttttttgaaa gctgttctac ttcttggtat cttttccgag gaagttatcc aacatgctgg
     4321 tgaattccat cgggaagatg tatttggtgg aaggactcat gcccatggct ttgagggttt
     4381 caaagtattg cagggtcatg gtcttctggt ccacagtctt ggctgagttg taaatctgct
     4441 caagggcttt tgcgaaacct tcggcgcgga gcagctgggc ttcgcgttca ccttccgcct
     4501 tgagaatggc cgattgcttt tcaccttcag cacggagaat gttggactgt ttttcacctt
     4561 ccgccacatt aacggcggct tcgcgtgtac ccgtggattc ggtcaccacg gcgcggcgga
     4621 tacgttcggc agacatctga cggttcatcg cgtcttgaac atcgcgcgga ggaatgatct
     4681 cgcggatctc tacgttggtc actttcacgc cccagcgctc agtgacttca tcaaggcggg
     4741 tgcggagcat gttgttgatg tgttcacgtt cggagagcac atcatcaaga gggataccac
     4801 cgatgaccgc gcgcaaagtg gtggccgcaa tacctgccgc cgccgattca aagttaccca
     4861 cctgcaaaac cgactgtgcg gggtcaagca ccttgtaata ccacaggaag tcgatggaga
     4921 tcggggcgtt atctttggta atggaggtct gatgaggaac ctcgcgcacc tgttcacgca
     4981 aatccacttt tacggggcgg tcagcaccag ggatgagcag gacgatacca gggcctttgg
     5041 gcttatccaa tacacggccg aggcggaaca caaccaatcg ttgatactct ggcacaatgc
     5101 gcaggaaatt gaacgccaaa aatacaacca cagccaggcc cagacataaa aataaagcac
     5161 ctaccgaacc aagaacacca ttcatatgtt ttctccttgt atgaaatgat tattgttttt
     5221 ccacaaccag ggtaaagcca tcacgccgaa tcacgcggac atgactccca gctggaattt
     5281 cagaatcgct gcgtgcagac cacatctccc cagcaatata tacgctccca tcgccataaa
     5341 tggctgactt cgcctcaccg atctgtccca ccaacgtctc aaggtcatgg gtgggtctgc
     5401 gggagatcgc ctcaatggct ttgcgaaata aaaaccacag aaaaacagcc accaaaccgg
     5461 aagagatcaa ggcaatgacc gggttcactg aaatgaggct gccatcatca ctggcaaaaa
     5521 ggaacaccga cccaatcact atcagcaaaa tggacagcgc caaaaaaaga aaactttttg
     5581 gttttcgtat ggcttgaatg aacggcacca cgctcagcaa caaaataacc agagcccacc
     5641 aattgaccga caggttatac aacgcatacc ctgtcaatat aaagcagaac aaggcgccga
     5701 tctccaccaa tcccgtccct ggggtggcta cggctataaa gaccaacaca atgccagtga
     5761 gaaatatcag ataggcaacg ttcgggtcaa gtaaaaagtc cattgttcct ccatttgatt
     5821 atacaacaag aagcaaggtt tcgattcggt atacggaaaa tgtcgtgcga aggttgcgtc
     5881 agactttgcc gcaagcggtt gtataataca cttctgcaaa aacaccatcc aggagcccta
     5941 gtgaaattcc aaaaaatctg tgtcattggg ctgggctaca tcggcctccc cacagcaacc
     6001 acattcgccg cacacgggat cgaagtgaca ggcgtagaca tcaaccaaaa tatcattgaa
     6061 accctcaaaa agggcgagat tcatattcat gagcctggcc tgcgtgatgg tttcgtcagc
     6121 gcagttcaat cgggcaggtt caaagccgcc accagccccg aagaagcaga tgccttcatc
     6181 atagccgttc ccaccccatt tcaggaaaac gaatacggct cacacaatgg gatgacctac
     6241 aaactggccg atatgcgtgc ggtgatttca gccgccgaat ccatcgtgcc gttcttgagg
     6301 aaggggaatt tggtggtgct cgaatcgacc tcaccgccac gcaccacact cgaccttgta
     6361 gcccccatcc tcgctcgttc tggacttgag gctgggcgcg acttccattt ggcatattct
     6421 cccgaaaggg ttttgcccgg gcagatcctg cgcgaactga tcgaaaatgc acgcgttatc
     6481 ggcggcataa cccctgagtc cgccgaggca ggctacgagt tgtattccac tttcgttaaa
     6541 ggcaagatca tcaaaaccga cgccaccacc gcggaaatgg tcaaattgat ggaaaacacc
     6601 taccgcgatg tgaacattgc catcgccaat gaattctcgc gccttgccaa taaattcggc
     6661 gtggatgttt gggaggcgat ctcactggcc aacctgcacc cgcgtgtgaa aatccttaat
     6721 ccagggcctg gtgtcggtgg tcactgcatc agcgttgatc cctggtttct ggtggaagcc
     6781 gcacccgaat tgaccccgct catttaccac tcaaggcggg ttaatgacac tcaacctcat
     6841 ttcgtcgtgg agaccgtcaa acgcgcattg ggcagcctca acggcaaacg catcgccgcc
     6901 cttggcctgg cctacaaacc cgacgtggat gaccttcgtg aaagccctgc ggctgaggtg
     6961 gtccacctcc ttcaacatga aggcgcacag gtcaaggcct gggagccttt caagccgaac
     7021 gcaaacatga acggcataca catgtcgcca aatcttgaag atgccattca ggatgccgac
     7081 accattttgc tgctggtcag gcacaccgaa ttcaaacaga tcagcccccg cgagatcgcc
     7141 ggcaagacca gtgcaaaaat catcgtagat actgtcaaca gctgggaagg cgaagcctgg
     7201 aagagcagcg gcttcgaatt cttccgcatc ggcgacagca aatcaccgaa ctcgtaaacc
     7261 gatcacccat ccttgtgttg aggcacttgt aacgttgaaa atcctctctg tttttggaac
     7321 ccgccctgaa gccgtaaaaa tggctccagt tgtaaaactg ctggaaaaaa ccgcaggcat
     7381 cgagtcaaag gtctgtgtca cagcccagca cagacaaatg ctcgatcagg tccttaacct
     7441 gttcgagatc cagccagatt atgacctcga cttgatgcgc gataaccagt cgcttgccca
     7501 gttgagcgca aatattttca cccacctcga tcctgttctg gatgatttca aacccgattg
     7561 ggtgctggcc caaggtgata cgacaacggt tgccatcacc tccctgcttt cgtacttccg
     7621 ccgcatcaag tttgggcatg tggaggcagg cttgcgcaca cacgacaaat ggcagccctt
     7681 ccctgaagag gtcaatcgcc gcattgccgg tgtcactgca gatatgcact tcgcccccac
     7741 cgaatggtcg cgtaacaatc tgttgcgcga gggtgtggaa gaaagcctga tcaaggtgac
     7801 gggcaacaca gtgatcgatg cactgcaatt cgtatccaaa caacctgagc ctgaagagat
     7861 cagacaactg gtaaaggaaa aactggcttc ttcacggctt attctcgtca ccgcgcatcg
     7921 cagggagaat tttggcctcc ccatggaagg tatctgtcat gctttgaaag agcttgcaca
     7981 ggaagaaggg gtcgagatcg tgtaccctgt gcatctaaac cccaatgtgc aggaacctgt
     8041 gaacaggatt ctcaaaggcg tggatcacat caccctcctt ccgcctctgg actacctgcc
     8101 gcttgtccat ttgatgaaac atgccaccat catcctgaca gattcaggcg ggattcaaga
     8161 agaagcccct gcattcggcg tgcccaccct tgttttacgc gacgtaactg aacgtccgga
     8221 gggagtccaa gcggggacgt taaagctggt tggaacagag accagccaca tcgttcagga
     8281 agcgaagcgg ctgttgtatg acaaatctgc gcatgcacaa atggcgaagg cagccaatcc
     8341 atatggtgat ggtcatgcgg ctgagaaaat catccatgcc ttgctggaaa atggtcagta
     8401 aaactaccag gttcccgcct tgcaattttt tttggaagat agccacctgt aatgtcaaaa
     8461 aagaagtttg taatgttaca cgaaaaaatt ctgagtataa tggaaacaga gatgacataa
     8521 gccatgcaac gcagatacct caccctgttt ctcgtcatat ttatgctcag tacaacctgg
     8581 gtacctgctc acgcgcaaga ccaaggctca aagtatttta gtgagactgg tcataatgtc
     8641 aaaggggatt tcctggtctt ttacaacagc aaccccaatg ccctcttcct gtatggttac
     8701 cccatcacag aagagtttat cgataaaaac aacaagacgg tccaatattt ccaacgtgcc
     8761 cgttttgaat atgacccgaa ccaacctgca gggcagcgcg ttcaactcac ccaacttggc
     8821 cgccaaacct atatctccac aggcaagtta gatgttgcga atacccttgc ctgccgcacc
     8881 tacagtgaaa ctggttttgc cgtttgtttt gcctttcttg aattctttga ccaatatggc
     8941 ggtttgaccc aattcggtta ccccatttca ggttttgaat atcacgagaa caagatcgtc
     9001 cagtactttg aaaaggcccg ccttgaatgg cagccttgga agacggaagg tcagcgtgtg
     9061 gttgtttcag acttgggccg aatttatttc gatgcgcttg gtgaagaccc tgggttgctc
     9121 tcccccgtca aaccgctgaa caacaccaat caggatgtga tcagcctgca agtccgtgct
     9181 tttgtttgga aggcggtcac cctcgccacc gactcacagc ttgtattcgt catcgtgcag
     9241 aaccaaaacc tgcaaccgat cgccaatgcc acctgctcaa ccattgtcaa ctggccggat
     9301 gggcgctcag aaaacatgtc gctgaccacc aacaccaacg gagtgggcat cgtgccgctc
     9361 tctttcgtca atcaaccata tggcagcctg atctacacag acgtttcctg ctcttatgga
     9421 aatctgagcg gcaagaccac cacatcgttc agaatttggt actaaattcc atctaaaaac
     9481 aacacagaac cgccctcgaa tgatcaattt cggagggcgg tttgatttta agatcaaata
     9541 tataacagtt tctattgttc ccctttgaaa tactccaccg tcgattggcc cgcctgattt
     9601 accccctctc tggcgatgac tttgccaacg gtcataagaa tgatgcggag atccagccag
     9661 aacgaccagt gatctacata ccatacatcc aggcggaagc gggatggcca gtcaagcgcg
     9721 tttcgcccgt tgacttgagc ccagcccgtc agtccgggca tggcatcatg tctgcgcatt
     9781 tgttcgggtg aatacaacgg aaggtattcc atcaacagcg gacgcggacc gacaaaactc
     9841 atctcacctc gcaaaatgtt gaagagttcg ggcaattcgt cgagcgagag ggaacggaga
     9901 atcctgccaa agcgtgtgag tcgctcggcg tccgggagca ggcttccgtc acgtgcgatg
     9961 cggtttgtca tcgagcggaa tttatagata ttgaatggac gtcccttgta cccgggtctc
    10021 tgctgggtaa atagaatcgg tgctccaata aaaacacgga cgagaatcgc agtaacgatc
    10081 aggacgggcg aaaaaatgat cagcgcgatc aacgcaaaga taaaatcgaa aatccgtttg
    10141 ctccatggca tgttcgtatt ataaccagac tttggaagtg cccaaagttt atggtatatt
    10201 ttgcagagga gtatagccat gaatgagcga atactaatca ttgaagacga tcaggcaatt
    10261 ctaaaattac tgcaacgggg tctggcatat gaaggctaca cggtggatac cgccaccgac
    10321 gggcgcatgg gtttgattgc cgcgcgcgac catacccccg atcttgtgat attggattgg
    10381 atgctgcctg gcatggatgg tcttgaagtt tgtaatcgcc tgcggactgg gggttcgatt
    10441 ccgatcttga tgctgactgc aaaggatact gtgcaggacc gcattcaagg cctggatgca
    10501 ggtgcggacg attacatggt aaagccgttc aatctggatg aattgctggc gcgtgtgcgt
    10561 gccctgttac gccgtacaca gcctgagcgc atccctgttt tgaaatttgc cgatctgtca
    10621 ctcgatactg gttcacggca ggctgcacgc ggaagccgca ttgtggcatt gaccgcgaaa
    10681 gagtatgaac tgcttgagtt gttcctgcgc caccccaaac aggtattgac tcgcgaggtc
    10741 atctttgacc gtgtgtgggg ttatgatttc ggcggtgaga gcaatgtgct tgaagtgtac
    10801 attcgctatc tgcgccaaaa actggagggc gaaggggagg cccgtcttat ccataccgtg
    10861 cgcggcgtgg ggtatgtgtt gagggaaaat ccctagttga aaagcggcag cattgacctg
    10921 ccgcttcttt tttgtatgac gatttctcag gttaccctaa actcacggga ttaaactttt
    10981 tacatgtcat tacgcctgcg cctcaccctt ttatattcca cgtttatgag cggaatcctg
    11041 ctcatttttg gcgcagcggt ctatattctc gtcaatgtga tcctgctaaa ccaggtggac
    11101 accatgctgg cgggtgttgt gcgtgatatt acgcaagtga ccaaggtcaa cccgatgggc
    11161 gaattaaact tgatcagcct gccacccctg gatatgacct cgaatgcata tgtgcaagtc
    11221 tggggacctg acaacaagat cattgcaacc tccccgagta tcggcacatt gaccaaaccc
    11281 cttgacccca tcggtcttga atcggacatg gtcgtttata aagattccta tttgagcgga
    11341 gcccatttgc gggttttgag tgtgccgctt aaagttggtg atcgcttcat tggcacgctg
    11401 caagtcggtg cgagtttgag cgtggtggat gccacgcaag gcaacctgct ttcgatgatg
    11461 gcagccattg cagtggttgc agttttgctg gctgggtttg gttcatgggt ggttttggga
    11521 cgcgcccttt ctccgctgga agccattaca gaaaccgtcg atcagatcaa ccgcgccgac
    11581 gacctttcgc gacgcattcc ttcacaggaa cgttctgaag acgagatcgg cgagttgatc
    11641 acttcgttta atcaaacgct tgaacgcctt gaatcgcttt tcacctccca gcaaagattc
    11701 cttgccgatg tcagccatga gctgcgtaca ccactcacgg tcattaaagg caacgtagac
    11761 ctgatgcgtc acttcaaaca ggtggatgaa gaatcgctca ccagcataga ccaggaagcg
    11821 ggtcggctta cccgccttgt cggcggcctg ctcatgctgg cacaggcgga atcaggcaag
    11881 ctgaccctcg tcatgaaacc cgttgaactt gatcttttac tgacagaagt gttcaccgaa
    11941 atgtgtgttt tggcgggctc caaggtgcat gtccacctca acgagattga ccaagtcatg
    12001 gtcaatggag atcgagatcg tttaaagcag gtgttgctaa atctggttgc gaacgcgatc
    12061 caatatacgc cgcagggcgg agaggttttc ctcagcatga agaagatcgg agaccaggcc
    12121 cgcctcatca tacgcgacac aggcccgggc atccctgcag aggacctgcc ccatatcttt
    12181 gatcgtttct accgtgctga aaaatcgcgc acccgctcca catccagtgg gtttgggctg
    12241 ggtctttcca ttgcgcaatg gatcgtggaa aatcacggcg ggcagatcaa ggttgaatcg
    12301 aaggaaggca aaggcacgac ctttgtgatc tggctgggaa tcgtgaaata gttttccaca
    12361 aaaaatgcat ggggatgatc gttttttcaa gcagccttta atcgaaataa gtttcagggt
    12421 actcgccgat cctcggtttt tcatcacgga tcgccaacag catggttgcc tcaccggggt
    12481 aatataaaat cccagtcggg cgcgaaataa caaagccctc gtattctgaa ttcaacacac
    12541 cgagagatcg tccccaggca tcgcgcatct ccaccaacgg atcaccaacc tttacgaggt
    12601 cacctggctc cttaagatgg tggacaatgc aggattgtgt cacccgtgcc acgcttcggc
    12661 gatgcgttgg aaagcccggg tcaacgacct tgatccccag gatcggctcc atttcaccgc
    12721 ccaacatgcc gccccagcgc atcacatttc gcagacctgc cacacccgca tggacaattt
    12781 caggggtggg cgtatatggg ctgctcaatt ccgccgtcag cgcagggatg cccgccagca
    12841 tggataccgc cgcagtagtg gcacggtgca atttctggtc gatatatttc tccacggcaa
    12901 aatcggcaat cacggtatga ccataggcct gtgccatctc atctattttt gtactcacaa
    12961 cttccgcacg ttggcgggcg gcctcatctc ccttgcgata taaaatacga tcacggaagg
    13021 tgaaagacaa agcgccattg ctcatacagt gcaggtcaaa atagaacgaa gccgtatttt
    13081 ttatttcctc aaataaacgc ccgtacgcct gttcaagcgc ggaaggcagg ttttggtctg
    13141 ggtcatttgt tttcggcttg cgcccatcag gaaagagacg gtttggatca cccgcatggt
    13201 aataggcctg acggttcaag gttcgcaaac cagcggggtt caaggcaggg atcgcgatta
    13261 tcgttccgcg catctgaccg accaactctg gtgtgatcaa cttgtgaatc acctgtatgc
    13321 ccgcatgctc aatgccgtgg atgccagcag taacccaaaa aacaggtcct tcttcctctc
    13381 cttgcgcaat gatgacaggc agagaatctg cgccgccaac ggggtgttgt accaaagcat
    13441 attcgccata agtgattccg ccgcgtttgg ccgcagctgt gccgatggta agtgccatat
    13501 aaaaaatcct tttgggtatg tttttgaata tcgtgagtat aatctgatta tgattaccgc
    13561 ccttaatttt ccaagtttcg atgagcattt ttcagactta aacaacttta ttttatatct
    13621 ggtacagcag tatcagacct atcggctggc ttcatgggaa gtccttgcac atcgttgccg
    13681 tcagttctac accccagatg tcatggatgc gattgaggca aaggcaccag ggtggaaaaa
    13741 aatggcttcc tactctgaag gcatcaccct tgaacatgtg acttgcgtat tcctcggcat
    13801 gtacatgctg cctgaatttc aagaattatc cgcagaacag cagcaaatgg caaaatggat
    13861 cattttgttc cacgatatag acaaggcgca tattcgcgga caaagagaca ccatgcatgc
    13921 ctttcattcg gcgatcgttg cagccagaga cctgcccggg ctgggctttc cagtgaccga
    13981 taaatatgcc agcttgatcg atgcatggag cgaatacacc cgccaggctt ttgtacaacc
    14041 cgcggaccag aattggccta aacccgataa tcaaaaactc cccaatatcc tttcaggtat
    14101 tgaagatatg ttcggagagc aggcccccgc cacgttgatc atcaaaaccg ccctgctgca
    14161 catttccctg cttgttgaca ggaactacca gacccctgcc cctttgaccg ataaggaagt
    14221 gaagcgtttt atctccccag ctttgtttcc acttttgcgg gttatgatgt tcgcggataa
    14281 cgaaggctgg tctctttttg acctggaatt ccgcgaccag caaaagcacg actcgtccat
    14341 cgagttcgag cggattcgaa aatttatttc tcccacctca tgacaaacaa atcatcccac
    14401 gatccatacg ccgcacttcg cctgcgggat tttcgccttt tgcttatcgg ccgcttcatc
    14461 acctcttttg gaaatgaaat gctttcattt gccattggct gggaactgtg gctgcgcacc
    14521 cgcagtgcat ttgcgctggg aatggtgggg ctcgtgcagg tggtgcctgt cattttatta
    14581 tccctgccgg gcgggcatgt ggctgatcaa tacaatcgca ggcgcatcat tgtcattacg
    14641 caggctgtct tttccctttg ctcggctggg ttggccctgc tttctttttt tgatggcccg
    14701 ctggttctga tctacctgtg cctgctgggg tttggtattg cgcgcgcctt caacgaaccc
    14761 gcctcctcca cactcattcc cgaaacggtt ccttctgaac tgtttgccag tgcggccaca
    14821 tggaacagca gcacctggca gacagcggct atttcaggcc ctgccatcgc tggcctcgtt
    14881 gcgggctttt taaacagcgt gaccattatt tatgtttttg atgcaattgc cgcacttgtg
    14941 ttcctgtttt tagtcaccca gattcacggc aaaccgcttc cgctttcaga gaaatctgac
    15001 acaatggcat cgctcatgga aggtttccgt tttatgcgcg acaccaagat cattctggca
    15061 gccatcacat tggatatgtt tgccgtgtta tttggcggag cagtggcttt gcttcccatt
    15121 tacgcaacag acattctcaa agtcggcgca cagggattgg gcatcatgcg tgcggcttca
    15181 tccattggag cgttgatgat ggctttcggg ctggcgcatt tgccgccgtt caaacaggcg
    15241 gggaaaactc tcctttgggc agtggcaggc ttcggcgcgg ccacgatcgt gtttggtcta
    15301 tcccgttctt tttggttatc ggtcgccatg ctggctttgc tgggagcatt ggataatatc
    15361 agtgtggtca tccgcagcac actgctcctc acacgcactc ccaacgaaat gcgcggacga
    15421 gtctctgccg tcaacagtat ttttatcagc acatcgaacg aaatgggcgc atttgaatca
    15481 gggctggccg cctctttatt tggaccgatc ctcgctgtgg ttgggggtgg aatcggcacg
    15541 atcattgtcg tcttaatcgt tgcaagggcc tggcctgaaa tgcgcaattt gaaaagtctt
    15601 gcagaccttg aagagccagc gcctgcccaa ttcacttcac caaaccaagg agacgaaaat
    15661 gccccttcaa gcacccaccc ctgatgaata tgcctcgttt tattctgatt acatccaacg
    15721 cgccgtggct cgtggcgatg ttctggccgc catgcccaca caaatagatg aacttgactc
    15781 tgctcttgga aacctgaccg acaaacaagc ccgcttccgc actggacctg aggaatggtc
    15841 catcaaagag atcgtcagcc atctgataga tgccgaacgg gttttttcct atcggctgct
    15901 gcgcgtttcc cgcaacgaca ctactcccct gccaggtttc gagcaaaatg gctatgtgag
    15961 cacctcaggt gccgatgatc ttccattggg agatctgctt gccgagtttt cgtttttgcg
    16021 acgtgccaac atccttgcca tcggtcacat gagcacagac gctgtgaagc gccgcggcac
    16081 tgtcagcggg gccactgtga gcgcaaatgc cctcatccat atgctggtgg ggcacgtaga
    16141 tcaccacatg gccagcctgc acgagaaata cctgccaaac cttcattgac cgaaggaaaa
    16201 gggggttttt cggggtttcc gtcacctggt gagcgattaa caattccctt gacaatacag
    16261 aacgtttgtt ctaaaattcg acctacctaa cccattggag gtcgaaatgg caaactatca
    16321 acgtaatctc ggcgatggct tgaaaaccat ctggaacaat ttcatccgca accgcaaatt
    16381 taatcgtggc gctgcctggg gcatgatgat catcggcgca ttgctgtcgt ttgaaatctt
    16441 caacttcagc acgacccaat ttgccctgcg cgacatgctc ggtgacctta cttttgcggg
    16501 gttccgttgg gccaccattc tggcgattgc cttctgcgga attgacttcg caggcattgc
    16561 ccgcatcttc acccccgagc agggacgcga cgaacctgcc gaagtatatt atctctttgg
    16621 cgcctggctt ctggcggcag gtttcaatgc cacactgacc tggtggggcg tttctgtagc
    16681 agtggctgaa aatgggaacg tacatgccgc tggtgcgctc agcgccgaaa ccctgaccaa
    16741 agctgtgccc atctttgtcg ctgtgatggt ctggctggtg cgtcttttga tcatcggcac
    16801 tttctctctt gcgggagatc gtctcttcac tacggccgac cagccgtcag cgcagcacag
    16861 accgcagtat caatctcaac cgcagacaca gcgcgcttac aacaatcccc aaccccagca
    16921 gcgccccgca tatcaaaatt cacagccgca gcgcccatct tataaccaac ctgtgaatca
    16981 gcccattttg cggcctgcct cgcaaattgc ccgccctgcg aacagcccaa gcacaaccaa
    17041 tttccgccct gcaccgaaac cgacggtcaa ttccacgcag acctctttca ttccgcctga
    17101 gccgacatac cacccgattt cctacgaagc ccgcaccaac agtgttgaaa gctccacccg
    17161 ccactacgac gcctaatcgt tgatgtcttc aaccgccatg gagtttgttg caacgcttcc
    17221 gccaaaataa tacaactgta taaataaatt ttcgagcacg ggtccttgag acccagaaaa
    17281 gagaatataa cgttatgccc cgcgctcgca catcgcaaat tgatcgccaa acagccgcgc
    17341 cccagcagga acaagggagc ggctgttttt ctggttttat gcttgtcccg ctttcggtca
    17401 tctttatcag tacgctgctt gcggcacttg ccatgaaaac gaccccgatc ccctcttcac
    17461 gtgtggattt gccgccttcc acttcgacag gcattgctct catcttcacg cctgagattc
    17521 aatattgggc agaagatatc gcccaatggg cggccgcttc gcaactggac ccgaatctgg
    17581 ttgccaccat catgcagatc gaatcctgtg gagatccacg tgccatttcc agtgctggag
    17641 ccatgggttt gtttcaagtg atgccgttcc atttcgccgc atctgactcg ccatacgacc
    17701 cagatacaaa cgccgcccgc ggactggcgt atctggtccg ctcgcttcag gcaggcggag
    17761 gagatgcccg ccttgcactg gctggttaca atggcggcat cggcatcctc agccgcgccg
    17821 aatggacatg gcccgcccaa acgaaaagat acattcaata cggcgtcccc atctatcagg
    17881 atgcacggaa cggcctacac catagtgagg ctgtcactga gtggcacgaa acatacggcg
    17941 ccagtctgtg ccgtcaggcg cataacaggc ttggattgcc gtaacttatt cttcaaacca
    18001 aggaaataag ggatgataga atcagccata ttgtgaacct tttattgaaa aacaagaaga
    18061 catatccagt ttaatcccag gccattaaaa atctcgtgaa aatctcaaac acactctatg
    18121 tgacggcccc caaagactgg cggacttggc tcaagcagca ttacaaaacc gaagccgaag
    18181 tctggctggt gtaccccaag aaagcgacag gcaagccacg catcgaatac aacgacgccg
    18241 tggagcaagc cctgtgcttc ggctggatcg actccatcat caagaccttg gatgacgagc
    18301 ataccgttca acgcttctca ccgcgcaagc caaaggctaa atattcgcaa gccaacatcg
    18361 agcgcctgcg gaaattggtt gcgcaaaaga aggtcatcaa agaagtagcc gatacactga
    18421 ccgacatcct gaacgccgag ttcatcattc cacccgatat cttgaaagcc attcaagcgg
    18481 a
//
LOCUS       DAOQLC010000086        18477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1048_length_18477_cov_23.303984, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000086 DAOQLC010000000
VERSION     DAOQLC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18477
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_18477_cov_23.303984"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..710)
                     /locus_tag="PL000_14470"
                     /pseudo
     CDS             complement(1..710)
                     /locus_tag="PL000_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
     gene            complement(855..1190)
                     /locus_tag="PL000_14475"
     CDS             complement(855..1190)
                     /locus_tag="PL000_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90156.1"
                     /translation="MDFKGVSYYPENELPKCQISISIYKQVKFISNLNYRHFGEKYIY
                     GDLKPKRKKKKIKYNKFYYIGCKKKEEAAKEILRLKKVLQELKNNLSKKKNNKFLNFF
                     IFLKKLFIN"
     gene            complement(1375..2103)
                     /locus_tag="PL000_14480"
                     /pseudo
     CDS             complement(1375..2103)
                     /locus_tag="PL000_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(2269..2790)
                     /locus_tag="PL000_14485"
                     /pseudo
     CDS             complement(2269..2790)
                     /locus_tag="PL000_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(2978..3049)
                     /locus_tag="PL000_14490"
     tRNA            complement(2978..3049)
                     /locus_tag="PL000_14490"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3015..3017),aa:Asp,seq:gtc)
     gene            complement(3328..4116)
                     /locus_tag="PL000_14495"
                     /pseudo
     CDS             complement(3328..4116)
                     /locus_tag="PL000_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
     gene            complement(4173..4427)
                     /locus_tag="PL000_14500"
     CDS             complement(4173..4427)
                     /locus_tag="PL000_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90157.1"
                     /translation="MNFYFKEISKYNLYFYNLNLNVNMNFISVSKIYNTILINKNYNP
                     YKLDIISQNSSILKACALYFDNLFRNYQNIENFYKKNKKR"
     gene            complement(4584..5480)
                     /locus_tag="PL000_14505"
     CDS             complement(4584..5480)
                     /locus_tag="PL000_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC90158.1"
                     /translation="MKKLGFLTFDKKINLNINDYYLYYLNPNFFEQLNNKKIFLFLGY
                     NLRLESPILNIKLRKKSMKENVLYLTIGSNFNDNLNSVMLGLNINTLIKYFQGKLKIC
                     NLILKNVKKIQNEIVSLSNLFNFNMFLIGNNVLNRVDNKNVFRLIEKYNKKNIVLNNR
                     YNNNSKNYLSFFFINFFINNLQYSSLKKGLNLGKNINVLYLNLTSILYEELNLHKNVH
                     NIETLAKNDIFYLLGLDFFKIKDSIFSVFQGHHLNLDYLKVDLIFPSITFLEKSSNFL
                     SIDGSLLQTNFILYPPVFCRND"
     gene            complement(5924..6247)
                     /locus_tag="PL000_14510"
     CDS             complement(5924..6247)
                     /locus_tag="PL000_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90159.1"
                     /translation="MPQLDIYIICNMLYGVIILFIFTYVLNINNILIIINLLLRLRKL
                     KLFLEKKYIFKNLQYILKKKNVIIYKFYGYFLSLKLNILKKIIFVENIKDIRNLKQSD
                     LKIND"
     gene            complement(7239..7988)
                     /gene="atpB"
                     /locus_tag="PL000_14515"
     CDS             complement(7239..7988)
                     /gene="atpB"
                     /locus_tag="PL000_14515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC90160.1"
                     /translation="MYYYQALEQFKVINYQVFGFNILPNYVLSIVVLILFFLFVTLLL
                     DVKNIMFLTRSFVQLIFEELYMFVLNVVKQNIGFLGIIYFPIVFNVFYVIYFINIGGL
                     IGYNFQLTSHIFVTFCISISLFIGVILIGIYNLKQKFLNQFIPKDAPKVLFPLLVVIE
                     TISYIIRPFTLGIRLFANIFSGHVLLFVIISFVIVIVEKKLIIVKLLLLVFPIMLLFF
                     IMTLELLIMFLQAYVFIILFCVYLNDSLHAH"
     gene            complement(8101..8439)
                     /locus_tag="PL000_14520"
     CDS             complement(8101..8439)
                     /locus_tag="PL000_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90161.1"
                     /translation="MKKVVDIKRADKKINRVKFGTFIGILHFLKKIIYNGYYFNEIYN
                     YFLYKNKKLSYNLFKDLDKGIIEFMGHLSIVSNIKNILYLFSKKNKSRIDYIVFIMIL
                     GIVIISIILL"
     gene            complement(8923..9450)
                     /locus_tag="PL000_14525"
     CDS             complement(8923..9450)
                     /locus_tag="PL000_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNC90162.1"
                     /translation="MEGPTPVSALIHAATMVTAGVFLLLRSVHLFKYDENSLLLLSFL
                     GGITSFIAATIGLFQNDIKKVIAYSTCSQLGYMVLSCGLNNYSGSLFHLMNHAFFKAL
                     LFLSAGAIIHSMLDQQDVRRMGMLLKYLPVSYIMILVGSLTIMGIPFLTGFYSKDFIL
                     EYVYSSNIYLSIFLY"
     gene            complement(10203..10517)
                     /gene="nuoK"
                     /locus_tag="PL000_14530"
     CDS             complement(10203..10517)
                     /gene="nuoK"
                     /locus_tag="PL000_14530"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC90163.1"
                     /translation="MILNLEIFKFLLISITLFIISIFGIFLLKRNLIVILMSIELCLL
                     SINFNFILFSVYLDDVIGQIFTLLILTVAAAESSIGLAILVMYYRRKGIIAINVMNYI
                     KG"
     gene            complement(10773..10844)
                     /locus_tag="PL000_14535"
     tRNA            complement(10773..10844)
                     /locus_tag="PL000_14535"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10809..10811),aa:Ala,seq:tgc)
     gene            complement(10855..10925)
                     /locus_tag="PL000_14540"
     tRNA            complement(10855..10925)
                     /locus_tag="PL000_14540"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10889..10891),aa:Asn,seq:gtt)
     gene            complement(11090..11359)
                     /locus_tag="PL000_14545"
     CDS             complement(11090..11359)
                     /locus_tag="PL000_14545"
                     /inference="COORDINATES: protein motif:HMM:NF013414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNC90164.1"
                     /translation="MNREKEKGLLRFQFNKGVCMTTRYKGYNTRFQLRSVSGKVAIEQ
                     EFLTYSRFNILMLLKLNPVKEFCRNSLKFLRNKKNKLSNYKVIIN"
     gene            complement(11810..11880)
                     /locus_tag="PL000_14550"
     tRNA            complement(11810..11880)
                     /locus_tag="PL000_14550"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11844..11846),aa:Phe,seq:gaa)
     gene            complement(11903..11974)
                     /locus_tag="PL000_14555"
     tRNA            complement(11903..11974)
                     /locus_tag="PL000_14555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11939..11941),aa:Met,seq:cat)
     gene            complement(11978..12056)
                     /locus_tag="PL000_14560"
     tRNA            complement(11978..12056)
                     /locus_tag="PL000_14560"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12020..12022),aa:Tyr,seq:gta)
     gene            complement(12072..12145)
                     /locus_tag="PL000_14565"
     tRNA            complement(12072..12145)
                     /locus_tag="PL000_14565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12109..12111),aa:Arg,seq:tct)
     gene            complement(12370..12654)
                     /locus_tag="PL000_14570"
     CDS             complement(12370..12654)
                     /locus_tag="PL000_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNC90165.1"
                     /translation="MEKFSKLIVIYSKHIEGLNLTSLSSARFIGAGSASAGLGGAGLG
                     IGVVFGCLLNAYSRNPNLKRELFAYAILGFALTEAMGLFCLMMAFIILFI"
     gene            complement(12740..12825)
                     /locus_tag="PL000_14575"
     tRNA            complement(12740..12825)
                     /locus_tag="PL000_14575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12789..12791),aa:Leu,seq:tag)
     gene            complement(13180..13515)
                     /locus_tag="PL000_14580"
     CDS             complement(13180..13515)
                     /locus_tag="PL000_14580"
                     /inference="COORDINATES: protein motif:HMM:NF012538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC90166.1"
                     /translation="MFLNNEKLYLQFYGLGYNSLLPSYVFASNVKETKSCIFEAVVLN
                     IPCSTLMDSNMGLYGVFYGLPGNDDNFLSVYLFTKLFIKMYFKSIFDNINSLKIEEND
                     LDFYEENIN"
     gene            complement(13830..15155)
                     /gene="nuoN"
                     /locus_tag="PL000_14585"
                     /pseudo
     CDS             complement(13830..15155)
                     /gene="nuoN"
                     /locus_tag="PL000_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
     gene            complement(15396..15478)
                     /locus_tag="PL000_14590"
     tRNA            complement(15396..15478)
                     /locus_tag="PL000_14590"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15442..15444),aa:Ser,seq:gct)
     gene            complement(15646..16008)
                     /locus_tag="PL000_14595"
     CDS             complement(15646..16008)
                     /locus_tag="PL000_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNC90167.1"
                     /translation="MKKKRPNYTRDYIVRLHQLLRFRVYYGDMTIVKFRRYLQRINTR
                     RLDFETKICFLLETRLDILLFRLNFVKSPRDARQYIKCRKIKVNDKIAISLNHQLYIH
                     DVVTFEEDVIPVFLKNLE"
     gene            complement(16270..16355)
                     /locus_tag="PL000_14600"
     tRNA            complement(16270..16355)
                     /locus_tag="PL000_14600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16318..16320),aa:Ser,seq:tga)
     gene            complement(16362..16432)
                     /locus_tag="PL000_14605"
     tRNA            complement(16362..16432)
                     /locus_tag="PL000_14605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16398..16400),aa:Gln,seq:ttg)
     gene            complement(16535..>18477)
                     /locus_tag="PL000_14610"
     rRNA            complement(16535..>18477)
                     /locus_tag="PL000_14610"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         7484 a         2563 c         1520 g         6910 t
ORIGIN      
        1 aataaagata attgtctata agcaactgca tgtttactta aatcatcata aataattaaa
       61 caaccagcac ctcttgtcat aaaatattca cctaaactag ttgcacaata aggtattaaa
      121 tattgtaatg cacatgattc agaagcagtt gctgctaaaa ttacactata actcatagca
      181 tctttttttc ttaataaatg tgctaatttt gcaacattag ctctacgttg acttattgat
      241 acataaatgc aatgtacatg tgttgcagga gttcaattat tattactttg atttaatata
      301 gtatcaatac ctatagtagt tttacctgta gatttatctc ctattattaa ttcacgttgt
      361 cctcttccta ttggtattaa accatctaca gatttgatac cagtttgtaa aggctcatta
      421 actttataac gtgatattat acctggagct tttatttcta ctttatattt taaatatgca
      481 tatttaaatt tatcatttga atctatagga tcaccataag cattaacaac tcttcctaat
      541 aattgagtag ttacagggaa ttcaaataat tctttagttc ttactacttt aaaaccaggt
      601 ttaactttat cttctccttt taaaattata gcttttatac cttcttcttc aatattaata
      661 actattgctt ttatatcttc taaacctgaa acttgtaaaa tttcacccat taaaacatta
      721 tccatgtttg atataaatgc tataccatca attaaacttt ttattatacc tggtataaca
      781 gtttgtaaaa cttctgaata aatatctcaa ctttttacta attttacgct aaattccatt
      841 taaattattt gatgttaatt tataaataat ttttttaaaa aaataaaaaa atttaaaaat
      901 ttattatttt tttttttaga taaattattt tttaattctt gcaatacttt ttttaatctt
      961 aaaatttctt tagcagcttc ttcttttttt ttacaaccaa tataataaaa tttattatat
     1021 tttatttttt ttttttttct tttaggtttt aaatcaccat atatatattt ttcaccaaaa
     1081 tggcgataat ttaaattact aataaatttt acttgcttat aaatgcttat agaaatttga
     1141 catttaggta attcattttc aggataatat gaaacacctt taaaatccat aaccatattt
     1201 ttatctaata taaaatttgg agtaaatatt acatttttat taacacttaa atctttttta
     1261 taaattttta aatgtttttg gctatctcaa aattttttat ttggataact tttgctatcc
     1321 aaaaattttt tattagatct aatcatatca ccgttttctt ctcaatattt ttgattaatt
     1381 tttttcatct ttttttaaac gtattaaatc aaaaataaca tataaaaata taataatact
     1441 aattattgtt attgcagaac ctgcactaga taccatattt aaagtataat aagcatctgg
     1501 ataatcaggt atacgtctag gcattccagc catacctaaa aaatgcattg ggaaaaaagt
     1561 taaatttaca ccaataaaag tagttcagta atgtaattca cctaattctt tattaaaatt
     1621 tttaccaaaa attttagaag atcaataata aaaaccacca aacataccaa acacagcacc
     1681 cattgataaa acataatgga aatgagcaac aacataataa gtatcatgta atatttgatc
     1741 taaacctgca cttgataaaa ttacaccagt aaatccacca atagtaaata atattaaaaa
     1801 acctatggca aataacattg gaacagtaat agttaaaata cctcttcata aagttgcaat
     1861 tcaactaaat atttttatac ctgttggaac agcaattacc atagtagctg ctgtaaaata
     1921 agctctagta tctatatcca tacctatggt atacatatga tgagctcata caataaaacc
     1981 taatacagca atagataaca ttgcgtaaac cattccatta taaccaaaaa ctcttcgacg
     2041 agataattga cttactattt ggcttactat accaaaagct ggtattatta atatataaac
     2101 ttctggatga ccgaaaaatc aaaataaatg ttgaaataat actggatcac ctccaccact
     2161 aacttcaaaa aatgcagtat taaaatttct atcagttaat aacattgtta taccagcacc
     2221 taatacaggt aaagataata ataataatca agctgttata aaaactgatc atacaaataa
     2281 aggcaatcta aacattaata aaccttttat tctcatattt attatagtaa caataaaatt
     2341 aattgcacct aatattgatg aaataccagc taaatgtaaa ctaaatatac caaaatcaac
     2401 agcactacct gaatgtgaaa aaatacctga taaaggagga tatacagttc aacctgtacc
     2461 tacacccatt tcaataattg atgatcaaac caataaaaaa aaagaaggta ttaataatca
     2521 aaaacttata ttatttaatc ttggaaatgc catatctggt gcacctacta aaacaggtac
     2581 aataaagtta ccaaaacctc ctatcattgc tggcattacc ataaaaaata tcattataaa
     2641 cgcatgtgca gttattataa cattgtatac ttgatgattt ccatctaaga atgcgaaagt
     2701 aatatcactt aactctattc ttatgatgac tgataacata gtaccaacta atcctgcaaa
     2761 caatgcgaat attaaatata aactgcctat atctttatga tttgttgata ataatcatcg
     2821 aaacataatt tttaaagata tatttaaatt tatatttaat attcaaaaat ttttatgctt
     2881 ataacctaaa aaataaaaaa attactttaa ttttgccccg ttaggagatt ccataggggt
     2941 aacttttatt aaaactatta acttttaact aagatacccc tgaagatggg agttgaaccc
     3001 atatctttta gttcgacaaa ctaatgctcg accattaagc tacttcaggt gatattaatt
     3061 taaattttat tacttttctg aaaaataata aataccttct caataaatac cagtatataa
     3121 aatcactaca cctaaagtaa tcggtaataa aatttttcaa cccaagcgca ttaattgatc
     3181 atatctataa cgaggaagag aacctctaac tcaaataaaa gtcacaacta acattataac
     3241 ctttaaacta tataaaaaaa tactttcata acttgaattt attaaaaacc caccccaaaa
     3301 aaaaataata tttaaacaac atactgttca aatagaaaca tattcaccta aaaaaaaaaa
     3361 accaaaagcc atcgcagaat attcaacatt ataacctgaa actaattcag cctcagcttc
     3421 tggcaaatca aaaggaggtc tattagtttc tgctaaagtt gaaataaaaa ataaaaaaaa
     3481 aaaaataaaa caaggtatta tatttcataa atgtgcttga cttcatatga tttcaaaata
     3541 atttaaagaa cttgtttgaa ttaaaatata aattaaagtt aaaccaatag atacctcata
     3601 cgctattact tgagcagctg ctcttaaagc acctaaaaaa gcatatttag aattactaga
     3661 tcacccagac attataatac catatacagt taatgaagat aatgcaaaaa acattaaaat
     3721 acttacacgt acatcagcaa atactgaatg gtttgaaaat ggtataatac ttcaatttaa
     3781 taagcttaaa attaaagtta ataatggtgc tattataaaa attattctat ttgataaaac
     3841 tggaattata gtttctttaa ctattaattt tacagcatcc gctataggtt gcaacaaacc
     3901 taaaaaacca actaaattag gacctcttct tttttgaata ctacctaaaa cttttctttc
     3961 tattaaaata gcataagcta ctaataataa taaacataaa acaacaacta aagcagataa
     4021 aacatttgct atcaaaatta atatatcatt atttaaatta taaatattta ttatttcgct
     4081 ctccccttta ctaaagatat aatcttttaa gtacatatat aatatactta caaaaaaaaa
     4141 ttgtattatt aaatttaaaa aattagttaa tttcatcttt ttttattttt tttataaaaa
     4201 ttttctatat tttgataatt tctaaataaa ttatcaaaat ataaagcaca tgcttttaaa
     4261 atactactat tttggcttat aatatccagt ttgtatggat tataattttt attaattaaa
     4321 atagtattat aaattttact aacagaaata aaattcatat ttacatttaa atttaaatta
     4381 taaaaatata aattatattt tgaaatttct ttaaaataaa aattcatact taaacttttt
     4441 aataaaaaac ttaatttttt aaaatctgat attaaaaaat aaaatctatt aacatttaaa
     4501 taatttattt ttttttttca atccaattta tataaagtac ttacaaaatt taaaatataa
     4561 atatataaag catttaaaat acttcaatca ttacgacaaa aaacaggcgg atataaaata
     4621 aaattagttt gcaataaact accatctata cttaaaaaat tactagattt ttctaaaaaa
     4681 gtaatacttg gaaatattaa gtccactttt aaataatcta aatttaaatg atggccttga
     4741 aatacagaaa atatagaatc ttttatttta aaaaaatcta aacctaataa ataaaaaata
     4801 tcattttttg ctaaagtttc aatattatga acatttttat gtaaatttaa ttcttcgtat
     4861 aatattgaag ttaaatttaa atataaaaca ttaatatttt tacctaaatt taaacctttt
     4921 tttaacgaac tgtattgtaa attattaata aaaaaattaa taaaaaaaaa agataaataa
     4981 tttttagaat tattattata tctattattt aatacaatat tttttttatt atatttttct
     5041 attaacctaa atacattttt attatcaact ctatttaaaa cattatttcc tattaaaaac
     5101 atattaaaat taaataaatt agataaacta actatttcat tttgtatttt ttttacattt
     5161 tttaatatta aattacaaat ttttaattta ccttgaaaat attttattaa agtatttata
     5221 tttaaaccta acataacaga atttaaatta tcattaaaat tactaccaat tgttaaatat
     5281 aaaacatttt ctttcatgct tttttttcgt aatttaatat ttaatattgg agattctaat
     5341 cttaaattat aacctaaaaa taaaaatatt tttttattat ttaattgctc aaaaaaatta
     5401 ggatttaaat aatataaata gtaatcattt atattaagat ttattttttt atcaaaagtt
     5461 aaaaaaccta attttttcat taatacttta cttgttacaa ttaactcata atcaacaaat
     5521 aatgaattta aaaaaattaa atttgaactt ttataaaaaa aactagaaaa tcaaactttt
     5581 aataaaaaat aataaaaagc ttgaattcaa cttatatata ccataatatc attttttttc
     5641 attaaaggta tattaatacg tcatttagtt aatccctcaa aaaaatatct tgttttattt
     5701 gaaattcatt cttcattaat aaaatcattt tttaaaggca acactcgttt tacttcagca
     5761 ccagaaatat aaatcttaat attactaccg aaagaatcat agatatcgat agtattatta
     5821 acatttaatt ctcacattcg ccctgaaaaa gcgtataatt tatctgttaa tgcacctata
     5881 cgtattttat taatcacaaa ttttacttaa ataattttac cattcagtca ttaattttta
     5941 aatcagactg ctttaaattt cgtatatctt ttatattttc tacaaatata atttttttta
     6001 aaatatttaa ttttaaactt aaaaaatatc cataaaattt ataaattatt acgttttttt
     6061 tttttaaaat atattgtaaa tttttaaaaa tatatttttt ttctaaaaac aattttaatt
     6121 ttcttaaacg taataataaa ttaataataa ttaatatatt atttatattt aacacataag
     6181 taaatataaa taatataatg acaccatata acatattaca tattatatag atgtctaatt
     6241 gaggcattta tttaatagct acagaagtag aagttcatca atatacacaa acatataaaa
     6301 acaatcatac aacatcaaca aaatgtcaat atcatatagc aaattctaaa cctaaatgat
     6361 gtctaatacc aaaatgacct tcacgtaatc taaaaaaaca aactaataaa aaacaagtac
     6421 ctaaaataac atgaaaacca tgaaaacctg ttgccataaa aaaaatacta ccatatacag
     6481 aatctgatat attaaaacct gcttcagtat attcaacaac ttgataataa gtaaaatgta
     6541 ttgctaatac tatagttcat attaaacctt ctcaattaac atctaaatca gatttattag
     6601 aaacaatatt atgatgacat caagttacac taacacctga taataataaa acaaaagtat
     6661 tatataaagg aacatgtaaa caattaaata cttgaatacc aaaaggaggt caaaaacaac
     6721 ctatttcaat acttggtgat aacgcaaaat ggaaatatgc tcaaaaaaat ccaaaaaaaa
     6781 acataatctc agaaacaata aaaagaatca tacctaattt taaaccttgt tgaacaggtt
     6841 ctgaatgttt accttcataa gttgcttctg atacaatatc ctcaaatcaa catgttacta
     6901 caataattaa aagtattaaa gaacttacaa taatatcact tgctattaca tatttattta
     6961 ctcataagat aaaacccatt aaaagattaa ataatgttat tgatactaat atcggtcaag
     7021 gagatggatc gataatgtga aatgaatgac ttcttttatt tgacatattt atttaatttt
     7081 attttttttt tacttgatat tatacttttt tctacttttt ttttaataac actttttaat
     7141 aaagaatatc taaattttac tctacaattt tttttttttg gtactgccat ttctttaaaa
     7201 tttttctttt tttattaaat aaaaattaat tttatttact aatgtgcatg taatgaatca
     7261 tttaaatata cacaaaataa tataataaaa acataagctt gtaaaaacat tattaataat
     7321 tctaaagtca taataaaaaa taataacata ataggaaaaa ctaataataa taatttaact
     7381 attattaatt ttttctcaac aataacaatt acgaaactaa ttataacaaa taataataca
     7441 tgacccgaaa aaatattagc aaataaacga atacctaaag taaaaggtct tattatataa
     7501 gatatagttt ctataactac taataatgga aataatactt ttggtgcatc ttttggtata
     7561 aattgattta aaaatttttg ttttaaatta taaataccta ttaatattac acctataaat
     7621 aaactaatag agatacaaaa agttacaaat atatgacttg ttaattgaaa attataacct
     7681 attaaaccac ctatatttat aaaataaata acataaaaaa cattaaatac tattggaaaa
     7741 taaattatac ctaaaaaacc tatattttgt tttacaacat ttaaaacaaa catatacaac
     7801 tcttcaaata ttagttgtac aaaacttctt gttaaaaaca taatattttt tacatctaat
     7861 aataaagtta caaataaaaa aaataatatt aatactacta ttgataacac atagttaggt
     7921 aatatattaa aaccaaaaac ctgataattt ataactttaa attgttctaa tgcctggtaa
     7981 taatacatcc caaaaatatt tatattttat ttattattta ttattattat taatatttat
     8041 tatttctaat acaattaata ataataaaat accaaatact tttaaattac ctaataaatt
     8101 tcataataat ataatactta ttattactat acctaaaatc ataataaaaa caatataatc
     8161 tattctactt ttattttttt tagaaaataa atataaaata ttttttatat tacttacaat
     8221 agataaatga cccataaatt caataatacc tttatctaaa tctttaaata aattataact
     8281 taatttttta tttttatata aaaaataatt ataaatttca ttaaaataat aaccattata
     8341 tataattttt tttaaaaaat gtaaaatacc tataaatgta ccaaatttta ctctattaat
     8401 ttttttatca gctcttttta tatcaactac ttttttcata tttaaagcta cattattact
     8461 aattttattt tgacgtctta aagaaaacgc aaaaataaat tgaaaataat aatacaattt
     8521 atatacaaat tcaacaataa atatataaaa tttaaaaaaa agaaaactta acaatgctga
     8581 tattaatgtg catattattg gtattatttt tattctcata ggtaaaaatt ctacatcaaa
     8641 tattcttaaa ttatctttat aagattcagt atctaaagta tttgttcata catcggtacc
     8701 catacctata aataaatcct taaataaaaa acctacaaat atacttccta aaaataaaat
     8761 aattaatgca attaaaatta aaaatttact ttcatgaatt acaattaatc ttttattttt
     8821 attatataag atattaaaat aactattgtg aacattataa aaagttaaaa ataataattt
     8881 aatagaataa aaagatgtta atgatgctga tattatacct aatcaatata aaaaaatact
     8941 taaataaata tttgaagaat aaacatattc taatataaaa tccttcgaat aaaaacctgt
     9001 taaaaatgga atacccataa tagttaaaga accaactaaa atcattatat aacttactgg
     9061 aagatatttt aaaagcatac ccattctacg tacatcttgt tgatctaaca tagagtgtat
     9121 aattgcccct gcgcttaaaa ataataaagc tttaaaaaaa gcatgattca ttaaatgaaa
     9181 taaacttcca gaataattat ttaaaccaca agataaaacc atataaccta attgactaca
     9241 agttgaatat gcaattactt tttttatatc attttgaaat aaacctatag tggccgcaat
     9301 aaaagaagta ataccaccta aaaaacttaa taataataaa ctattttcat catatttaaa
     9361 taaatgtaca gaacgtaata ataaaaatac tccagcggtt accatagtag ctgcatgaat
     9421 taatgcagaa actggtgttg gaccttccat agcatcaggt aatcaagtat gtaaacctat
     9481 ttgagcagat ttggcacaag cacctaatat aaataaaaat aaaataagtg ataaatactc
     9541 tacatttatt caaaaaacat aagtatttaa ataaacataa cttctaaata atcaagataa
     9601 acaatttatt acaaaaaaat ctaaactaaa aacatgacaa aataataaaa ccatagctaa
     9661 taataaagat atatcaccta atttatttat tattatagct ttcattgcag ctttattagc
     9721 tgcaattcta gtagatcaaa aatttattaa taaatatgaa agtacaccaa caccttctca
     9781 acctataaat aattgtatat aattatcaga tgttactaaa aatatcataa aaaaagcaaa
     9841 agacgataaa aaagctaaaa atcttattaa atgtggatct ctatacatat attctacaga
     9901 aaaaaattga acagctcaag atactatagt aataactaat aacataatac tacttaaatt
     9961 atcatataca aattctattt ttacatccat taaacctaaa tttattcaag gacttatgct
    10021 tattataaca aaaacttcag ataaaaaagt ttttcaaaat attcataaag ccgcaactac
    10081 agttaaaaaa ctacttaata aactcatatt agctgctccc atttttccta gtcttgatcc
    10141 taaaaaaaaa agaattaaag cattcattat tggaaaatat aaaactaata aatacatctt
    10201 ttttaccctt ttatataatt cattacattt atagcaatta taccttttct tctataatac
    10261 ataactaaaa tagctaaacc tattgaagat tctgcagctg ctacagttaa tattaataag
    10321 gtaaatatct gtccaattac atcatctaaa taaacagaaa ataatataaa attaaaattt
    10381 attgataata aacataattc tatactcatt aaaattacaa ttaaattacg ctttaataaa
    10441 aaaattccaa aaatacttat tataaataaa gtaatggaaa ttaataaaaa tttaaaaatt
    10501 tctaaattta aaatcatttt tttttttttt tttttttaat aaataaaagc ttaatataaa
    10561 agctttcaaa aagtttataa aaaatctaaa tttttttatt tagataaaaa gaaaattaat
    10621 ttcttttttt tttattattt ttcagtattt tttaatattt aaagttaata atataaatta
    10681 tataaatatt tatttttaca attttatttt attttttgga aaatttttta tttaactttt
    10741 tttgctactt ttgaataaaa tttaacataa attggagcag gacggaattg aaccgacatc
    10801 atatacttgc aaaacatata ttttaacctt ttaaactact gctctaattt acgaatcaga
    10861 tcgggatcga accgatgacc ttgtggttaa cagccacacg ctctaaccta ctgagctact
    10921 gatttgcctg cactgggaat cgaacccagg acctctgcgt taaaagcgca tcgctctacc
    10981 aactgagcta tgcaaagtct aggagtggac ttgaaccact atttttcgat ttgcagtcga
    11041 atacattgcc aattatgtta cctaatttat tatataactt tataattacc taatttatta
    11101 taactttata attacttaat ttattttttt tattacgtaa aaattttaaa ctatttctac
    11161 aaaattcttt tactggattt aattttaata acattaaaat attaaatcga gaataagtta
    11221 aaaattcttg ctctatcgct actttaccac ttacagacct taattgaaac ctagtattat
    11281 aacccttata ccttgtagtc atacaaactc ctttattaaa ttgaaatctt aataaacctt
    11341 tttctttttc tctattcata tatcaaacaa ctatataagc accagataat gctaaaaaac
    11401 attttatata ttgttttttt tttttactct cattatattg attaattctt attttttcta
    11461 attgatttat tataaacatc tttatatttt attattaatt atatatttta ttttatattt
    11521 tattaattca aacaacaaac gatatattct tttatctttt aaaccaatct taattaaatc
    11581 cttaacattt ttatattttc ttaaaaataa taatttatca gtatctatta ataatatttg
    11641 aattttatca gtgtatacaa aattacctaa ttttaataat tttttacttt tattaaacat
    11701 tttataaata taattaacat atttgcccac tattggactt gaaccaatac ctagaaacct
    11761 agagtaaatt ttaagtctac cgtgtctacc atttcaccaa gtggcttgct tagagatgga
    11821 atcgaaccat cgacctttgg tttttcagac caacgctcta accttctgag ctatctaatt
    11881 tattttactt aatattatct ttatgagggg aaaggacttg aacctttatt tttaggtcat
    11941 gagcctaacg agtttccgtt tactccaccc cttattcgga gagtaggatt cgaacctacg
    12001 aaagaaaacc taacagattt acagtctgtc gccattaacc actcggccat ctcctttttt
    12061 atttcaaatt atgcacccta taggattcga acctacactc tattacttag aaggtaattg
    12121 ctttatccaa ttaagctaag ggagctaaac ctaaatattt tttaaatata cattgtataa
    12181 aaagtataaa tatttttcaa aataaatatt tcaatatatt tatactatat gcccatacta
    12241 aaattcccaa tctataaatt atataaaatc aagtgcaata ttcggctaat tataatattt
    12301 aggatttaat aagaggtttt aataaacctc ttattttatt aattcaatta ttaaaaaatt
    12361 ataatattat taaataaata atattataaa agccatcatt aaacagaata atcccatagc
    12421 ttcagttaat gcaaaaccta atatagcata agcaaataat tctcttttta aattaggatt
    12481 tcttgaatat gcatttaata aacatccaaa aacaactcct atacctaaac ctgctcctcc
    12541 aagacctgca gatgcactac ctgctcctat aaatctagct gatgataaag aagttaaatt
    12601 taaaccttca atatgtttgc tataaattac aattaatttt gaaaattttt ccattttatt
    12661 aaactttaaa attaaaaaaa aaaatattag taaaacatat tgataattat tttatttaga
    12721 actttagtta tttttttttt acggataaag ggatttgaac ccttacagta tataactacc
    12781 acaaggacct aaatcttgcg tgtctaccaa tttcaccata tccgttttat ttaatttacg
    12841 gcagagaagg gatttgaacc cttaacaatg attttgaaga ccactatttt accattaaac
    12901 tactctctct agattaatta attttattta tttattatgc aatttacctt ttaacttaat
    12961 ttgcttttta atttttttag cctttttttt acgagctttt atacttaatt ctatacatct
    13021 ttttttataa aaacgaaaaa acataatagt attattttta aattttcttt tttttaatac
    13081 attttgcatt catggatatt ttttaaatct tttattttga ataaaaggtt tatatactct
    13141 tcaatttatt gcgttatggc ctttttcata tcttaatttt caatttatat tttcttcata
    13201 aaaatccaaa tcattttctt ctatttttaa actatttata ttatcaaaaa tacttttaaa
    13261 atacatttta ataaataatt ttgtaaataa atacacactt aaaaaattat catcatttcc
    13321 aggtaaacca taaaatacgc catataaacc catattactg tccattaatg tactacaagg
    13381 tatatttaaa actaccgctt caaaaataca agattttgtt tcctttacat tagacgcaaa
    13441 tacataagat ggtagtaatg aattataacc taaaccataa aattgtaaat aaagtttttc
    13501 attatttaaa aaaatttctc tttgattgct taaagcacca ccaattcatt tattattaat
    13561 atatcataat tgttttactt ttatatcttt aaaatctgtt cctattttat aaccaatatt
    13621 attagctatt aataattttt gacctaataa acatatatta taagatacat ataaagcttg
    13681 ctttaatata aataatgtat aatataaatt aattatatga attgaattac gcagtcctaa
    13741 taaaaaataa gataaacgat aatcacattc ccatttataa tgtcccaaat gaacaccatt
    13801 ctttataaac tgttttatag tataaagcat taaataaata aaaataataa aatattatga
    13861 attaatatta ataaataact aggataacaa aaaaaaaata tatttaaaaa aactaaaaaa
    13921 actaaaaata aagtttttaa ctcacctaaa tcaactaaaa ataaaaattt attttgaatt
    13981 gatgaataca tcatttttat taatcttaaa taatatataa cacctatact actaaaaatt
    14041 attaaaaata aagaaaaaaa atacattttt aattctatta tatttaataa aataaataat
    14101 ttactaaaaa aacctatcat tggaggtata cccattgatg aaaataaaaa aataaaaaaa
    14161 attattgaaa tatatttatt agaattaaat aataatataa tttcaccaat ttcttttaat
    14221 tttaatttat tactacgcga tctaaaactt aataataaaa ttcataaacc actagacatt
    14281 atattataca ctaatatata aaaaataaca ctatacatac cttcaatatt tatagttaac
    14341 atcatactta atatgtaacc catattacta atactactat aagcaattat cctttttaat
    14401 ttagtttggt aaattgctcc aaatgtacct accactattg ataacataga taaaaacata
    14461 aaaaaaatat ataaatcttt taaataagta taaacaaaaa taatatttaa acgtaataat
    14521 aatgataaaa tgctaatttg aggaacaacg gcaaaaaaaa aagtaatatt tgttggagat
    14581 ccttcataaa tatctgaaac ccacatatga aatggcacta taccaatttt aaataaaata
    14641 ccacaataaa taaaaataag accaataaaa gagctttcat aataatataa attgtaaccc
    14701 tccatattat aataataaaa taatactgtt aaatctaaaa aattagttaa accagtaaaa
    14761 ccatataata atgaagaacc aaataataaa atacctgaag ataatgcacc taatataaaa
    14821 tattttaaac cagcttctat tgaaaaattt gaataaaatt ttaaatttgt cacaatataa
    14881 aaacataaac tttgtaattc aatagctaaa tacattgtta ttaaatcata acttgaaatt
    14941 atagttaata tacctattat cgataatagt aataaaaaaa tatattcata tatttttata
    15001 ttttcatatt cacaataatt taaagaaaat aatatactaa aaaaacttgt taataaaacg
    15061 atgaatttaa aaaaaaaaga taaattatca caaattattt gataatttaa taaaataccc
    15121 tcgaaatgca cagtagtata agataaaact atcattgcga aaaataaaat taaagaacca
    15181 aataattgca ccatatttaa aatattaaat ttatacctta aatttaatat tttctcttgt
    15241 aatataacac taaaaaataa taagaatatt atatgtacta taatataaat ttctgtactt
    15301 aataaaaaat aaccgaacat gatttttaat aaaattatta attgtttatt gctttttata
    15361 ggattattaa tttttttttt tttttaataa tccctctggg gtgggattcg aacccacgat
    15421 atttattaaa tatatcgact tagcagatcg acgctttaaa ccactcagcc acccatttta
    15481 attaaatttg taaaattaaa aattttgtaa aaaagataaa ttaaaatctc aagttgttgg
    15541 aatattttta cgtgtaggat atgaaattaa catacatttc ataattaaac tacaaaattc
    15601 caaatattta ggataattaa ataatatttt atggtttcgt atttttcatt ctaaattttt
    15661 taaaaaaact ggtataacat cctcttcaaa tgtaacaaca tcatgtatat ataattgatg
    15721 atttaaacta attgctattt tatcatttac ttttattttt ctacatttta tatattgacg
    15781 cgcatctctt gggcttttca caaaatttaa acgaaataat aaaatatcta aacgtgtttc
    15841 taataaaaaa caaatttttg tttcaaaatc taaacgtctt gtatttattc tttgtaaata
    15901 tctgcgaaat tttactatag tcatatcacc ataatatact ctaaacctta ataattgatg
    15961 taatcttaca atataatctc ttgtataatt tggacgtttt tttttcatag acatcattct
    16021 tggataataa aattttaaat aaggtttttt atataaaaaa gtaataaaac gtactttata
    16081 tttatattta ttacaaaata ttttaaacat ttttttaaaa aaacgttttc catttgtgca
    16141 tgatgttaaa taacctcaaa aatcaacttc atatttattc ataaatttat attttaatga
    16201 accttttttt ttcattttat tatatttttt aaacttaaat tttttaatta aataatattt
    16261 taatttaagc ggaaagagtg ggattcgaac ccacgacatc gtatagatga ttgattttca
    16321 agatcaacgc tttaaaccac tcagccatct tttacttaaa tttggagtaa taggattcga
    16381 acctatgatc atggtatcaa aaaccaacgc cttaccactt ggctatactc cttgttataa
    16441 atattatatt taatttttta attaattatt ttatttttat tattttcacg aatttttagt
    16501 acttctaagc caatatcttg caataatcac acatatacac aagcctaaaa tatcgtaata
    16561 atcttctact attttttaag aaaatttttc agaattattt tcccacattt attttttcag
    16621 cggttataac aattgtatgt ggctacttgg cattgctaat tagtttaaca accaatacac
    16681 catagataca tattaactga tcctctcgta ataagttaaa ctttctttaa ttttctactt
    16741 taacggtaga taggaaccga actgtctcgc gacgttctga acccaactca tgtaccattt
    16801 taatgggcga acagcccaac ccttgaaacc tatttcagct tcaggatatg ataagtcgac
    16861 atcgaggtgc caaacaaccc catcgataag aactctaaag gggaattagc ctgttatccc
    16921 tggcgtacct tttatccgtt aagcgataac ctttccattc agaattaccg gatctctatg
    16981 accgactttc gtctctgttt gatttgttat tcttacagtt aagcattttt aagccattat
    17041 actttataat taattgttaa ttaattataa aacacctttg cacgcctccg ttacctttta
    17101 ggaggcgacc gccccagtca aactaccaat cataaactgt ctaatattta ttattattat
    17161 tagtataaaa actatatatt aaatggtatt tcactgacgt ctattcaatt agcttgcgct
    17221 aaggaatact ttaatatttc tacttatatt taaacaagaa tgttaaacta cactaaatac
    17281 tttcaaaata tttaaacgac tcccatctat tctacatttt atattttttt tatacaacta
    17341 atgattatag taaaggtgca cagggtcttt ccgtctagcc gttaatattc tgcatcttca
    17401 cagaaatttc aatttcactg agacaatatt ggagacagtg aggaagtcgt tacaccattc
    17461 atgcaggtcg gaacttaccc gacaaggaat ttcgctacct taggaccgtt atggttacag
    17521 ccgccgttta ctgggattta tattcaaaac ttttaatatt gaaattaata tttatattaa
    17581 taataaattt atagtgttac ctctaaactt attattaata taaatattaa ttaaaaataa
    17641 tttataaagg tagtacttta taatattaca atttaaattt ataaaactta tttataaatt
    17701 taccaataca attatttctc ctttttatct tacagcaccg ggcaggtgtc agaccctata
    17761 cttagtaatt atactttgca gagtcctgtg tttttgttaa acagtcgcta ccccctgttt
    17821 cgtgacactt aaataaactt cttatttaag cactctttct cccgaagtta cagagttaat
    17881 ttgccgagtt ccttcaatat tgttacctca tacaccttag tatactctac ctattcacct
    17941 gtgtcggttt tagtacggtc atttcaaaat gtagctattt cttgaaattc tttatattat
    18001 atctacaatc taataaaggt ttacgatatt aaatatttca aaatttgtca cttcatttgg
    18061 cttctacaac taaatattaa tagtattccc atcaaaatta gtggactact ttattttttt
    18121 ttagggaccg gcttactcaa tttagtttat ctaaaaattg aaaactttga attttcggtg
    18181 ataatgttaa ggcaatatat ttaaatatcc attattttat gttactcatg tcagtattct
    18241 tgcttctgat tttttaaaat aatttttcaa ttattctttt ataaaaatta cagaatattc
    18301 tgctaccata tatattttat ttataaataa tttacaaaag atatatatat atttataatt
    18361 tcggtatata ttttaaactc cctacattta tcgattttaa tataaattta tttaaaaata
    18421 agtttttttt cagtgagctg ttacgctttc tttaaaggcc tgctgcttct aagccaa
//
LOCUS       DAOQLC010000087        18408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1056_length_18408_cov_23.776222, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000087 DAOQLC010000000
VERSION     DAOQLC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1056_length_18408_cov_23.776222"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PL000_14615"
     CDS             <1..1317
                     /locus_tag="PL000_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC90168.1"
                     /translation="AKHGAKPEELVTDPNKRSETPPEWVSQAQSIGEAQSAQKAEEAP
                     IQSEAVENALNIGEQFFAEFENASKGIPSSSTTGTEPSTDAWLREFEETENQAVFSSA
                     EDSFEKQEQPRSDIPDWLNESHPSETDGQFAFEKSGSQKSDLPDWLNVEENAFPQDAS
                     AASQNDLSNWLSSLDNEPGLPFEEMPTPDSIYNASKAVSKRKPADEEPVMKKPDLPEW
                     LSNVDADDQWQAPASESTEEPVTASENENLPDWLHNLEEEEAVVVDEDSPRLVDHPVS
                     SSDWKPMANQTPEPKKEVSPVEPRKPFVPPPAEPRAEAEVQPQQMERPVEERRTEKPQ
                     SATSRKKASVSAKQRQPEAQGTSASLSQAKGELDRGDIPAALDHYKKLIKRGKHIEET
                     IRDLTESIYRYPVEVGIWQTLGDAYMRANRLKEALEAYNKAEELIR"
     gene            1381..1833
                     /gene="ndk"
                     /locus_tag="PL000_14620"
     CDS             1381..1833
                     /gene="ndk"
                     /locus_tag="PL000_14620"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC90169.1"
                     /translation="MERTLVLVKPDGVQRGLIGEVIARFERRGLRLVAAKFIQVSTTL
                     AEAHYAEHKGKPFYDGLIQYITSAPVMAMVWEGSNAVAAVRQTVGSTKPAESAPGTIR
                     HDFALEVGRNLIHASDKPETGEREVALWFKKDELVDWKRAVDQWIFEK"
     gene            complement(1906..2223)
                     /gene="rsfS"
                     /locus_tag="PL000_14625"
     CDS             complement(1906..2223)
                     /gene="rsfS"
                     /locus_tag="PL000_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC90170.1"
                     /translation="MVNSLEDKKGEDIVLIDLKDIVSFTDYFVLCTGTSDRMLDALAN
                     STIDNIHSAHRKKGKKQGISSDGWLVIDYGDVVVHLFSPDQREFYNLEDLWKDGKVLL
                     RLQ"
     gene            complement(2259..2843)
                     /gene="thpR"
                     /locus_tag="PL000_14630"
     CDS             complement(2259..2843)
                     /gene="thpR"
                     /locus_tag="PL000_14630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNC90171.1"
                     /translation="MSLLRVFIATEFPSNTLEAIEKQTAKLRLTLGNEIIRWVPTQNM
                     HLTLKFIGDIAASHLDFLKQLLTREAESHKPFDLQLGGLGSFPNPRRPRILWLGLHAP
                     AELLSLQKSIEAGAARLGYQQEERPFSPHLTIGRTRQNIGLPEFQKIRTALDATQLGN
                     ISPARMEAIHLFKSDLQPGGSVYTKLFSAPLSKP"
     gene            complement(2852..3232)
                     /locus_tag="PL000_14635"
     CDS             complement(2852..3232)
                     /locus_tag="PL000_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90172.1"
                     /translation="MTTLQKTVMIIEDEPEAAELFGEMMRVNGFRVMKMFSSAPAIPM
                     IIQEKPDVIILDIMMPDISGLEVLRYMRREPDLATIPVIVISAKSMPSDIKTGLEAGA
                     SLYLTKPVGFHELKQAVEQVLQNA"
     gene            3362..3868
                     /locus_tag="PL000_14640"
     CDS             3362..3868
                     /locus_tag="PL000_14640"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HNC90173.1"
                     /translation="MTDLEDFRAEKDDFFGSHPQSPLTREQRMKFKGLNYFPENESLR
                     LEVKVEEFSTKDAFEMQTSTGGVQHYERFGRFHFHVDGVEAALTIYQSPHGFFLPFVD
                     ALAGVETYPAGRYLEPEALPGGRFLVDFNVAYNPYCAYNEMWSCPITPAENRLSVAVR
                     AGEKLFHE"
     gene            complement(3920..4204)
                     /locus_tag="PL000_14645"
     CDS             complement(3920..4204)
                     /locus_tag="PL000_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90174.1"
                     /translation="MDNNTSLQNNQEDRNTQEQLQDEVVLKFLRVLESIQAEELSCSE
                     TFARLDEFVETEVQSKDADKITPLIREHLDMCPECNEEYEALLAVLENTK"
     gene            complement(4238..4876)
                     /locus_tag="PL000_14650"
     CDS             complement(4238..4876)
                     /locus_tag="PL000_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC90175.1"
                     /translation="MTTRTNEIWLADLRANGPSHAEALNDLRDVIQKGLPYALSRWLS
                     SDNPLFQPLVEEVTQETLLRVLDQLDSFEGRSLFTTWVHKIAIRIALTELRRKRWRDA
                     SLDELTENEDAPPPPGLLADPQASPETSSERSDMLVRVRRILEEELTPKQREALVLLG
                     LQDMPMEEAAKRMKTNRNALYKLLHDARLRLKKRLALEDITPQDLLTAFEQK"
     gene            complement(5006..6433)
                     /locus_tag="PL000_14655"
     CDS             complement(5006..6433)
                     /locus_tag="PL000_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNC90176.1"
                     /translation="MFKIKFGTDGWRGVIAEDYTFDNVRRCAQGFATYMLSVGKKGQW
                     VIVGHDKRFSSEHFAAAVAEVLAGNGLKVYLTQEATPTPVIAYAVLDKKAAGAVNITA
                     SHNPPTDNGFKVRNETGGAIDPDGLKLIEASIPDDLKDVKRKEYKEAVAAGEIVKFDA
                     ATPYIEHLTRDGLIDLQPIKDAGLTVMVDTMWGNGAGWFPRLLAGGKTRVIEIHNERN
                     PSFPEMKRPEPIQPNIDVGLKATVDNKADVLLITDGDADRCGIGDENGEFINQLRVYA
                     LLAYYLLEVRGERGDIVKTLSTTGMLNKLGELYNVPVHETGVGFKFVAPKMTETNALI
                     GGEESGGYAFRGNVPERDGILAGLYMLDFMVKTGKKPTELLKDLFAKVGGEYFYDRVD
                     SPFSGDRAAREKIITDNNPQTLGGLKVTELITVDGFQFKLEDGGWMLIRFSGTEPILR
                     VYCETRHKDKVQGILQDGLKVAGIK"
     gene            complement(6483..7925)
                     /locus_tag="PL000_14660"
     CDS             complement(6483..7925)
                     /locus_tag="PL000_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90177.1"
                     /translation="MQIHEQISHQLQMNKPAKLNSSMQVYLFALLAAVMVAFVYGWLN
                     FPSLKKSGTNYYPQLAIGFKNGELSLPEQPSEALLSLSNPYNYVLRKESGVEDFPWDA
                     SLYQGRFYIYWGPVPAALLTVLPVGLLNKIGDQHLVFPFVFGVFIYSLLIVVELWSRL
                     EQRPPAWALGLALLALGIASPATLMLSRAKIYEASISASQFFFLGGCYWAFRALGSNE
                     KNIFWQLVLAAFHWALAIGSRTIILPAAAFVGVVTIFFIWKKNTAFFTPSLFAMGAPI
                     FIVALGLCWYNWARFGSVFEFGLRYQLTTTDYNEFNNLFSVRYLRENLLSYFLRPYQI
                     QAEFPFISTVENTVSNDRLAGLPYTSPHLLFLLLPLARFFSPHARAEDRQETWLINAL
                     GGASLISLVIILLYYFTAMRFSFDFMPGLLVLAAIHFMQGYGQAKSNQGYLLLAVVLF
                     SFSILLSTLIALPAVRIREVFKIFELLGIR"
     gene            complement(7995..9056)
                     /locus_tag="PL000_14665"
     CDS             complement(7995..9056)
                     /locus_tag="PL000_14665"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC90178.1"
                     /translation="MSDFLNYLNTADLDTLTKTPGITRPIAGGIIAGRPFDSVDDCLK
                     VRGMGKNLLSRLQSNFEGEGNASESRAMVTVEEEPKPAPIERSRPAQESAREEEPSFL
                     TRLGQAFLVFLRALMRLIALAIIIVGIGAAFYYGLPFINQKVIVPIEQNTVGIRELND
                     QIEILQTQLDEVNARVDAVEKTIETQSASIAQLEEMQKTLEEEMTTQNNSVMVALKRE
                     VMYTRAIETLSRARLYLSQSNFGLAKTDVQSARDLIAELSLDAPTYQVDAFNQILMRL
                     DLALGNLPAFPVIAVDDVDIAWQLMMMGLPESEAEIVPTATPLPPTLTPTVVIEPSAT
                     VTLTPAPVLEITPTPTAIP"
     gene            9135..>10267
                     /locus_tag="PL000_14670"
     CDS             9135..>10267
                     /locus_tag="PL000_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNC90179.1"
                     /translation="MIYLDYAATTPVDSRVFDAMLPYFRETYGNPSSVHRYGQKAESA
                     IEEAREKVATVLHCRADEIIFTSCGSESDNLALRGAATARRRASGQKWILTSKTEHHA
                     VSKTAEQLEKEYGFSLEWLDVNEFGGVSPEALLKAVCSDTALVSVMYANNEIGTINPI
                     AELAGIAKVNNILFHTDAVQAAAYLDVNTQTLGVDLMSLGGHKFYGPKGVGALYIRKG
                     TKLLPHMTGGGQEYSLRAGTQNVPYIVGFAEALHLAAEEREMRTAHVKPLRDHIIGMV
                     LETIPDSQLTGHPTNRLPNHASFVFKNVDGNLLLQMLDAAGFACSSGSACKTGNPEPS
                     EVITALGHSRDWALGSLRITLGVDSTPEQVDSFVKELPGLVEKA"
     assembly_gap    10268..10367
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10368..11858
                     /locus_tag="PL000_14675"
     CDS             <10368..11858
                     /locus_tag="PL000_14675"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HNC90180.1"
                     /translation="LKTQIITLESHDDLISVRDKLSWAKTPRILLVWPKYAKVTLRIL
                     DLKVLQRHADSLGAQLGLVTRRAKVRRDAESLGIPVFASTTTAQRELWPDPVPRSTRT
                     PKPPRRDLREIRDAVYQKEPAWRTSLLGRVLTFTVGVMAFVTVAGLFVPRAALTLYPE
                     TQVQSIVIPVAASESAASISLAGAVPALTLSVTEQAEQSLAIVSEISVPKSKAKGVVQ
                     FTNLSQNELSIPAGTVVATAAEVPVKFTTLEDATLAAGIDEVVEIPIEAQQAGTDGNV
                     PNDAVIVVEGPLGLTVSVTNPEPVTGGVISQVTGANDDDRARLREVMLENLRRMAETE
                     LRSKIGPADVLLVDTLEIKQVLSEAFDPPAGQPGKTLVLNMQVEFSARYVSDDAMREL
                     SLATLNAAGQDGFVSTSAPDYKVITEPSTDNSGISHFELMVTRTLLREVDEMRVFSIV
                     RGGKPDLMKAELASALSLRQSPDILLTPSWWPWMPLIPFNISVEMK"
     gene            11896..12321
                     /gene="ruvX"
                     /locus_tag="PL000_14680"
     CDS             11896..12321
                     /gene="ruvX"
                     /locus_tag="PL000_14680"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNC90181.1"
                     /translation="MRILAVDHGEKRIGLALSDPTATIASPLKVVEHVSRMIDAAQVA
                     EIAAQNEVGLIVIGQSYDDDGNPNPAGRRAGRFADELKKQTELPIEMWDESFSTQDAR
                     AVRIELGLSRKKRGGHQDEFAAVAILQSYLEAKRGSGNP"
     gene            12321..13484
                     /gene="mltG"
                     /locus_tag="PL000_14685"
     CDS             12321..13484
                     /gene="mltG"
                     /locus_tag="PL000_14685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC90182.1"
                     /translation="MSDSQIVNRKSPSKNFLRRYQLHLILAVVVFCSLTCLYFALGYA
                     PARASLLYGPPASALSISDRIEYSARLLAYGDTLRSPYDPNGFEQSFFVEQGESVFSI
                     STRLEQAGLISNAQAFYDYAVYAGIDLTIQSGEYTLSPALSIIDIARALQNFSPSDAT
                     LVILPGWRMEEIAASIPTSGLEFSPEAFLALAQTPPPALEFVPSSSSMEGFFFPDSYT
                     LPRSTTPEQLLDVVARNFVQHLTDDLRTGFANQGLDIHQAVTLASIVEREAIHHEEAP
                     LIASVYLNRLAIGMKLDADPTVQYAVGYNLFQETWWTNPISAADLEFDSPFNTYLYIG
                     LPPAPISNPSLEALHAVAYPETSGFYYFRAACDNSGYHVFAATLEEQVANGCP"
     gene            complement(13533..14693)
                     /locus_tag="PL000_14690"
     CDS             complement(13533..14693)
                     /locus_tag="PL000_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90183.1"
                     /translation="MKIKNLLALILLLLSTLACGESTVSAAPTPACTATSPAVKLDAE
                     SRPFHMGFTRWPPEATLEGIDRMNNFVNTYGDMTALHFDGGVPWPEALTDSPLPAGLM
                     SSWQFTRDAIPAEHTLLVSITPLDMTRSKLAAYWGESDNQPLPKPWDGYSLDHADVKT
                     AYLNYARRVVEYFHPDYLAIGIEANVAQVNAPESWEAYKQLHQFVYENLKAEYPDLPI
                     FATFTISHMSGLDGGDQATQKKEIETLLPYLDMLGLSAYPYGWMYESGKADPIPDDFF
                     APALAFGKPIGITESGAPSYDFSAYGKDYKFSEEYQARWIDFLMQKGIENHFEFAIAF
                     TGIDYDKLIAGMPTDVRELATIWVYTGLQRSDGCDKFALSYWDAYLKLPFKK"
     gene            complement(14785..15519)
                     /locus_tag="PL000_14695"
     CDS             complement(14785..15519)
                     /locus_tag="PL000_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90184.1"
                     /translation="MSISQRLKTGFIFLLGAYLALTWLLVFFATRSDRVLHAIISMGS
                     GLLLFWVVLGGSGMVLFRNRIKAFVQGIPLNWQVKFILFGILLAMTEEAITTTMTNLA
                     PLFGVAIGEAYITASANYFDVILHHSVIVFVPWFFAWAWILKRYDFSPFWVFLLLGLQ
                     GLIGESIAFGAQNLSQFAMWIFVYGLMIYLPAYTIPDGRGARKPNLLHGMLAFFLPLL
                     CGFLWAILINLIFPDHPSIHFPPIQN"
     gene            complement(15634..16638)
                     /locus_tag="PL000_14700"
     CDS             complement(15634..16638)
                     /locus_tag="PL000_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90185.1"
                     /translation="METFKKSLAGLFAVLFVMSAVFALLLFNFEREGFSAETYRKAFA
                     KDDFYNRLPTVLAEAMVTTDQNQLPIVMSRMDVQAWDAFFRTILAPDALTTMGDGLLN
                     STFTYLNQQSDIVQLSLAPLKTSMVGPSGVQAIYGLLSTQPSCTLMQLAQMGIDLISG
                     GSIQFCNPPAELEPVMTPAIQAQLQVAAALIPDQITVYEAPLQNDPRQKLQTFRLFMR
                     LSPILPLGFLLLMTIFAVNSFTSWLTWWGASFLATGAFAGLMSFTGAPIFSFVFQGML
                     ASRLSDYLPSIFLAYASDLATAMLQALLSPVLWQGLSLSAIGLVMLLVGYFVKASQMR
                     "
     gene            complement(16708..>18408)
                     /locus_tag="PL000_14705"
     CDS             complement(16708..>18408)
                     /locus_tag="PL000_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC90186.1"
                     /translation="YMLPVEREFVSPEFVRLGTQLACAEQIRSGVTTFNDMYFFESDI
                     AQATAEAGMRAVCGQSVMKYPAPDAPAFEDSIAYAREFIKTWKGHPLIVPAIAPHAPY
                     STTPEILRTCTDIAKEYDVPLHIHISETAFEVENMRNEQGMPVVPYVKKQGLLEAKVI
                     AAHCVHIDVGEMKTLQHVNAGISHNPSSNLKLASGFAPVQKMLDVGLNVGIGTDGPAS
                     NNDLDMFEEIRLASFVAKASSNDPTVVPAATALLMGTRLGAQALHIGHLTGSLEAGKR
                     ADLIIVDTSPAHNSPRFRRDPHNAYAQLVYASKSTDVTDVMVNGKWLMRDHNLLTLNE
                     ADLIKEAQGVAKKIDTFLIEREKSVLSKLIALGGSMQEESFEVQVKVKIAEPDKLVQA
                     MKRPEIEITAQKHYQQFDDYFLFDDPSQGRLRFREDNLIDEKGNSQNVRSRLTLLGQK
                     REGEIGHDVLLSRSRFIAPAIHTARFYREYFKPAKEISITKDRLRWHIRYKNTELFIN
                     LDEIKEPPMGHFLEIKSRTWSRKDADLKAELVSELLDLLGAGHAEVVTQDYIEILTAS
                     "
BASE COUNT         4391 a         4700 c         4820 g         4397 t
ORIGIN      
        1 gcgaagcacg gagcaaaacc cgaagaactg gtcaccgacc cgaacaaacg cagtgaaacc
       61 ccacctgaat gggtttcgca ggctcaatca attggggaag cacaatctgc tcaaaaagca
      121 gaggaagcgc ccattcaatc tgaagctgtg gaaaacgcgc tcaatatcgg cgaacaattt
      181 ttcgctgaat ttgaaaacgc ctccaaggga atcccctcct catccaccac aggaacggaa
      241 ccatccacgg atgcatggct tcgtgaattt gaagaaaccg agaatcaggc tgtattttca
      301 tctgcagaag attcgtttga aaagcaggaa caaccgcgca gcgatattcc tgattggttg
      361 aacgaatctc acccatccga aacggatggt caatttgctt ttgaaaaatc tggttcccaa
      421 aaatcggatc tgcccgattg gctgaatgtg gaagagaacg ccttccccca ggatgcatcc
      481 gccgcttccc aaaacgacct ctcgaactgg ttgagcagcc ttgataacga gcctgggctt
      541 ccgttcgaag aaatgcctac accggattcc atctacaatg cttccaaggc tgtttcgaag
      601 agaaagcctg ccgatgaaga acctgtgatg aagaagccag acctgcctga atggcttagc
      661 aatgtggacg ctgatgacca atggcaggcc cccgcgtcag aatcgaccga ggaacctgtc
      721 actgcctccg agaatgagaa tctacccgac tggctgcaca accttgaaga ggaagaagct
      781 gtcgttgtgg atgaagattc gccgcgcctg gtagaccacc ctgtatcgtc ttccgattgg
      841 aaaccgatgg cgaatcaaac ccctgaaccg aaaaaggaag tatctcctgt ggagccgcgt
      901 aaaccctttg taccgccccc cgccgagccc cgtgctgaag cggaggttca acctcaacaa
      961 atggaaagac ctgttgaaga gcgccgtacc gagaaacccc aatctgcgac ttccagaaag
     1021 aaggcaagtg tgtctgcgaa acaacgtcaa cccgaagcgc agggaacttc cgcttcgttg
     1081 agtcaggcca aaggtgagtt ggaccggggt gatattcccg ccgcgctcga ccattacaag
     1141 aaactcatca aacgaggaaa gcatatcgaa gagacgatcc gcgacctgac cgaatcgatc
     1201 tatcgatatc ctgttgaggt gggtatttgg cagacattgg gcgatgccta tatgcgagcc
     1261 aatcgtttga aggaagcact cgaggcctac aataaagccg aagagttaat tcgctgatct
     1321 gttgaatcaa actttcgttg attcgctgaa tcgtcatata ttctatttgg aggattttct
     1381 gtggaaagaa cgctcgtact tgttaagccc gatggtgtgc aacgcggcct catcggtgaa
     1441 gtgattgccc gctttgaacg tcgtgggctt cgtttggtgg ctgcaaaatt tattcaggtc
     1501 agcaccacgc tggctgaagc gcattatgcc gaacataaag gcaaaccgtt ttacgatggt
     1561 ttgatccaat acattacctc ggcgcctgtg atggcgatgg tttgggaagg ttcgaatgcg
     1621 gttgcggctg tgcgccagac cgttggttcc accaaaccag ccgagtctgc tcctggcacc
     1681 atccgccatg attttgccct tgaagtgggc cgtaatctga tccatgcttc tgacaagcct
     1741 gagaccggcg aacgcgaagt tgcgctgtgg ttcaagaagg atgaacttgt ggattggaaa
     1801 cgcgccgtgg atcagtggat cttcgaaaaa taagcgtttc acgctgatat aaaaaatccc
     1861 gcccaatttt tagttgggcg ggattttttt gtgaaaatat tttttttatt gcaggcgcag
     1921 caataccttg ccatccttcc acagatcttc aaggttataa aattcacgtt gatccgggga
     1981 gaacagatgc acaacgacat cgccatagtc aataaccagc cagccatcac tggagatgcc
     2041 ctgttttttg cctttctttc tatgggcgct gtggatgttg tcgattgtgg agttggccaa
     2101 tgcgtccaac atacggtcgc tggtacccgt acacaggacg aaatagtctg tgaaggatac
     2161 aatgtctttc aagtcgatca ggacgatatc ttctcccttt ttatcttcca gggagtttac
     2221 gatttcacgg gcaagttcaa gagcgttcag ggttcacctc atggtttgga taatggtgcg
     2281 gaaaaaagtt ttgtataaac tgacccgcct ggctgcaggt cgcttttgaa tagatgaatg
     2341 gcttccattc tggcagggct gatattaccg agttgggttg catccagggc agttcgtatt
     2401 ttttggaact caggcaggcc aatgttttgt ctggtacggc caatggtgag atgcggggaa
     2461 aagggacgtt cctcttgttg atagcccagg cgggcggctc ctgcttcgat gcttttttgc
     2521 agggagagta attctgccgg ggcgtggagt ccgagccata gaatacgggg ccgcctcggg
     2581 tttggaaaag agccgagtcc gccgagctgc aagtcgaatg gtttgtgaga ttccgcttca
     2641 cgagtcagca gctgcttgag aaagtcaaga tgtgaggcag caatgtctcc gatgaatttc
     2701 aaggtcagat gcatattttg ggtgggaacc cagcggatga tttcattccc cagtgtcagg
     2761 cgcagcttgg cagtttgttt ttcaatggct tccagcgtat tggagggaaa ctcagtggcg
     2821 ataaagacgc gcaaaagact catgcgggat ttcaggcgtt ctgtaacact tgttcaacag
     2881 cttgcttgag ttcatggaaa ccgacgggtt tggtaagata cagggatgcg cctgcttcga
     2941 ggcctgtttt gatgtcactg ggcatgcttt tggctgaaat cacaatgact ggaatggttg
     3001 ccaggtctgg ttcacggcgc atgtaacgca aaacttcgag accagaaata tccggcatca
     3061 tgatatccag gatgatgaca tcgggttttt cctgtatgat catggggatg gcaggggcac
     3121 tggagaacat tttcatgaca cggaagccat taacacgcat catttctcca aataattcag
     3181 cggcttcggg ttcatcctca atgatcataa cggttttctg caaagtggtc atttttgtac
     3241 ctcatttact aaaatagcat aaactgcatg aattggcaag ggcggtctcg ttatcggaaa
     3301 tgttatcgta agaatcccgc ctgtttgcaa gtctatggtg cataacatta ttggaggaac
     3361 catgacagat ttggaagatt tccgtgcgga gaaggatgat ttctttggca gtcacccgca
     3421 gagcccactg acacgcgaac agaggatgaa attcaagggt ttgaattact tccctgaaaa
     3481 tgaatccctg cgccttgagg taaaagtgga ggaattttcc accaaggatg catttgaaat
     3541 gcagacctca acaggcggtg ttcagcacta tgaaaggttt ggcaggtttc actttcatgt
     3601 ggatggggtg gaggcggctt tgaccattta tcagagtccg catggatttt tcctgccttt
     3661 tgtagatgcc ctggcaggtg ttgagaccta ccctgctggt cgctacctgg agcctgaagc
     3721 cctgcctggc ggtcgctttt tggtggattt caatgtggcg tacaacccat attgtgccta
     3781 caatgaaatg tggtcatgcc caatcacacc tgccgagaac cgtttgtcgg tggcagtgcg
     3841 cgcaggtgag aagttgttcc acgagtagaa aagacaggtt aacaaaaaaa gcgacgggat
     3901 gtgaacatcc cgtcgctttt tattttgtgt tttcaagaac ggccaaaagg gcctcatatt
     3961 cctcgttgca ttccgggcac atatccagat gttctcggat aagcggtgtg atcttatcgg
     4021 catccttgga ctgcacttcc gtttcgacga attcatcgag acgggcaaag gtttcggagc
     4081 aggatagttc ctcggcctga atgctttcca gaacccgcag gaactttaga accacctcat
     4141 cctgcaattg ttcctgggta ttgcggtctt cctgattgtt ttgaagagaa gtattgttgt
     4201 ccatgctgtg gatttgacga ttaatcaaat cttaatctta cttttgttca aaggcggtca
     4261 gaaggtcttg gggggtgata tcttccaagg ccagccgttt tttcaggcgc agacgggcat
     4321 catgcaggag tttgtagagc gcgttgcggt tggtcttcat ccgtttggcg gcttcttcca
     4381 tgggcatgtc ttgcagacca agcagaacca gtgcttcgcg ctgttttgga gtgagttcct
     4441 cttccaaaat gcggcggacg cgaaccagca tgtctgaacg ctctgatgag gtttcagggg
     4501 atgcctgggg gtcagctagc aggccggggg gcgggggagc gtcttcattt tcggtcagtt
     4561 catcaaggga ggcgtcgcgc cagcgttttc gacgcagttc ggtcaacgca atacggatgg
     4621 cgatcttgtg tacccaggtg gtgaaaagac tacggccttc aaagctgtcc aattggtcga
     4681 gcacgcgcag gagggtttcc tgcgtcacct cttcgaccag cggctgaaag agtggattat
     4741 ctgaggaaag ccagcgggac agggcgtaag gcaggccttt ttggatgaca tcgcgaagat
     4801 cgttcagcgc ttcggcatgt gacggtccat ttgcccgtaa atctgcgagc cagatttcat
     4861 tggtacgtgt ggtcatgatg ttggattata agagaaaact accccgtgga cgaaaactct
     4921 tataaaactt tgagactttt tgatagcttc ttcaaaacga ccctgcctgt atatttggca
     4981 gggtcgtttt gaaatgtgaa gaaagttact tgattcctgc aactttcaag ccgtcttgca
     5041 ggattccctg caccttgtct ttatgcctgg tttcgcaata cacacgcagg atgggttcgg
     5101 taccgctgaa gcggatcaac atccagccgc cgtcttcaag cttgaattga aaaccatcca
     5161 cagtgatgag ctcggtgact ttcaagccgc ccaaagtctg aggattgttg tctgtgatga
     5221 tcttttcgcg ggcggcgcgg tcaccgctga acggactatc cacacggtcg tagaaatatt
     5281 cgccaccgac tttcgcgaac aggtctttca aaagctcggt tggcttcttg ccggtcttga
     5341 ccataaaatc gagcatgtac aacccggcaa ggatgccatc acgctcgggc acgtttccgc
     5401 ggaaggcgta gcctccgctc tcttcaccgc cgattagggc attcgtctct gtcatcttcg
     5461 gcgcgacgaa tttgaaaccc accccggttt catggacggg tacgttatag agctcgccga
     5521 gtttgttcaa catgcctgtt gtggacaggg tcttgacgat gtcaccgcgt tcgccgcgca
     5581 cttcaagcag ataatacgcc aacaaagcat agactcgcag ttggttgata aactcgccgt
     5641 tctcatcgcc aattccgcag cggtctgcat caccatcggt gatgagcagt acatcggctt
     5701 tgttatcaac ggtggctttc aagcccacat caatgttggg ttgaatgggt tccgggcgct
     5761 tcatttccgg gaaggatgga ttgcgttcat tgtggatttc aatgaccctg gtctttccac
     5821 ctgccaacag gcgcgggaac cagccagcgc cgttgcccca catggtgtcg accattacag
     5881 tcaaccccgc atctttgatg ggttgtagat cgatcaagcc gtcacgggtg aggtgttcga
     5941 tgtacggtgt ggcggcgtcg aatttgacga tctcgccagc tgcaacagct tccttgtatt
     6001 ccttgcgttt gacatctttc aagtcatctg gaatggaggc ttcgatcagt ttcaacccat
     6061 cggggtcgat cgctccgccc gtctcattgc ggaccttgaa gccgttatcc gtcggtgggt
     6121 tgtggctggc ggtgatgttg accgcgccag cggccttttt atcgagcacc gcatatgcaa
     6181 tgaccggagt gggagtggct tcctgggtca ggtaaacctt caaaccattt cctgccagca
     6241 cctctgcaac ggccgcagca aagtgctcac tgctgaagcg tttgtcgtgg cccacaatga
     6301 cccactgtcc ctttttgccg accgagagca tgtaagttgc aaatccctgc gcacaacggc
     6361 gcacgttgtc gaaggtgtaa tcttctgcga tcactccacg ccaaccatcg gtaccgaatt
     6421 ttattttgaa cattggccaa ctccttggat tgaaataaat tatggcatga cttcgacgaa
     6481 ttttatcgga tgcctaaaag ctcgaatatc ttgaaaactt ccctgattct gaccgcgggc
     6541 agggcgatca atgtactcag caatatggaa aaggaaaaaa gcacgacggc caaaagcaaa
     6601 tacccttgat tggatttagc ctgcccatat ccctgcatga aatggattgc ggccagtaca
     6661 agcagcccgg gcatgaaatc gaagctgaaa cgcatggctg tgaagtaata cagcagaatt
     6721 ataaccaatg aaatgagaga tgccccaccc agggcgttga tcagccaggt ctcttgccga
     6781 tcttcagcac gcgcatgcgg actgaaaaaa cgagccaaag gcagcagcag gaacagaaga
     6841 tggggcgagg tgtaaggaag ccctgcaagc ctgtcatttg agaccgtatt ttccacggtg
     6901 ctgataaatg gaaactcagc ctgaatttga tatgggcgca gaaaatagct cagcaggttt
     6961 tcccgcaaat agcggacgga aaacagattg ttgaactcgt tgtaatctgt ggtggtgagt
     7021 tgataccgca gcccaaattc aaacacagag ccgaagcggg cccagttata ccagcacaga
     7081 ccgagcgcga cgatgaagat cggcgctccc attgcaaaca gggatggagt gaagaaggct
     7141 gtgttctttt tccaaataaa aaaaatggtc accacaccca caaatgcagc agcaggcagg
     7201 atgatggtcc gcgacccgat ggcaagcgcc caatggaatg ccgccagcac aagctgccag
     7261 aagatgtttt tttcattgct tcccagtgcg cgaaaagccc agtaacatcc gcccaaaaag
     7321 aaaaattgac ttgccgaaat tgaagcctca tagatcttcg cacggctgag catcaatgta
     7381 gctgggcttg caatgccaag tgccagcaag gccagcccga gtgcccacgc aggcggacgc
     7441 tgctcaagcc tcgaccataa ttccaccacg atcaatagag agtagataaa taccccaaat
     7501 acaaacggaa aaacaagatg ctggtcgccg atcttgttga gcagcccaac agggagcacg
     7561 gtcaatagtg ctgctggaac tggtccccag taaatgtaaa acctgccttg atacagagat
     7621 gcgtcccacg ggaagtcttc cacgccgctt tcttttcgca ggacataatt gtaggggttg
     7681 ctcaaagata gcagggcctc ggaaggctgc tctggcaggg aaagttctcc gttcttgaaa
     7741 ccgatcgcca attgtgggta atagttggtc cccgattttt tgaggctggg aaaattcagc
     7801 cagccataaa caaaagccac catcaccgct gcaagcaagg caaaaagata aacttgcatg
     7861 gaggaattca gtttggcagg tttgttcatt tggagttgat gtgaaatttg ctcgtggatt
     7921 tgcatttcaa ttcaaaatgc gggcagatta taacattttg aaaaagaacc ccgagaattc
     7981 tcggggtttc gattttacgg aattgctgtt ggggtggggg tgatttccaa aacaggcgct
     8041 ggggtaagcg taactgtagc agatggttcg atcacaactg tgggtgtgag tgtcggcggc
     8101 aggggtgtgg cggttggtac gatctcggcc tcactctcag gcagacccat catcatcaat
     8161 tgccaggcga tatccacatc atctaccgcg atgacgggga aggctggcag gttgcccagt
     8221 gccagatcaa ggcgcataag gatctggttg aacgcatcca cttgataggt cggcgcatcg
     8281 agcgataatt ctgcaatcag atcacgcgcc gattgcacat ctgtttttgc cagcccaaaa
     8341 ttactttgcg aaagatacaa cctcgcccgt gaaagagttt cgatcgcgcg tgtgtacatc
     8401 acctcgcgct tcaaggccac catcacgctg ttgttctgtg ttgtcatttc ttcttcaagc
     8461 gtcttttgca tttcttccaa ctgcgcaatg ctcgcagatt gggtttcgat cgttttctca
     8521 accgcatcca cacgggcgtt cacttcatcc agttgagttt gtaaaatctc gatttgatcg
     8581 tttagctcgc ggatccccac cgtattttgt tcgatgggca cgatcacttt ctggttaatg
     8641 aacggcagcc cgtaataaaa tgccgcacca attcccacaa taatgatggc cagagcgatc
     8701 aaacgcatca aggcgcgcag gaatacgagg aaggcctgcc ccaacctcgt caggaaggat
     8761 ggttcctcct cacgcgctga ctcttgtgca ggccgactcc tctcaatggg agcaggcttc
     8821 ggttcttcct ccactgtgac catggccctg ctttctgaag catttccctc cccctcaaaa
     8881 ttggattgta accgtgacag caggtttttc cccatgccgc gcactttcag gcagtcatcc
     8941 accgaatcaa atggccgccc ggcaatgatc ccgcccgcga tcggacgtgt gatgccaggg
     9001 gtcttggtca atgtatcgag gtcggcggta ttaagataat tgaggaagtc actcattctg
     9061 cacctgattt cgattgattg tattttctta tctattttat caccccgata aaattgtttg
     9121 ctataatccc ttgcatgatt tatcttgatt atgccgccac aacccccgtt gactcgcgag
     9181 tctttgacgc gatgctgccg tattttcgcg aaacctatgg aaacccatct tccgtccatc
     9241 gttacgggca aaaagctgaa agcgccatcg aagaagcccg cgaaaaggtg gctactgttt
     9301 tacactgccg cgccgacgag atcatcttca cctcctgcgg ctccgaatcg gataaccttg
     9361 cgcttcgcgg cgccgccaca gcaaggcgcc gcgcctcagg ccagaagtgg atcctgacct
     9421 caaaaactga gcaccatgcc gttagcaaaa ccgccgaaca acttgaaaaa gaatacggat
     9481 tttccctcga atggctggat gtgaatgaat ttggcggcgt ttcgccagag gcgctgttga
     9541 aagctgtttg cagtgacacg gcactcgtct cggtcatgta cgcgaacaat gagatcggca
     9601 cgatcaaccc catcgctgaa ttggcgggca ttgcgaaggt gaataatatt ctttttcaca
     9661 ccgatgccgt gcaagctgcg gcttatttgg atgtgaacac ccaaaccctc ggcgtcgacc
     9721 tgatgtcgct tggcgggcac aaattctacg ggccgaaagg cgttggcgcg ctctacatcc
     9781 gcaaagggac caaactcctc ccacacatga cgggtggggg gcaggaatac agcctgcggg
     9841 cagggactca aaatgtgccc tacatcgtcg gttttgctga agccttgcat ctcgcagccg
     9901 aagaacgcga gatgcgcaca gcccatgtca aacccttgcg cgaccatatc atcggcatgg
     9961 tgcttgaaac gattcccgat tctcaattaa caggtcaccc caccaaccgc ctgcccaatc
    10021 atgcttcctt tgtcttcaaa aatgtggatg gcaaccttct gttgcaaatg ctcgatgcag
    10081 cgggctttgc ctgctcttct ggctctgcct gcaaaactgg caaccccgaa cccagcgaag
    10141 tcatcacggc cctcggtcat tcacgcgatt gggcgcttgg ttccctgcgc atcactttag
    10201 gagtcgattc cacacctgag caggttgatt cttttgtgaa agaattgcct gggttggttg
    10261 aaaaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttg aaaacccaaa
    10381 tcatcaccct cgaatcacac gatgacctca tctccgtccg cgataaatta tcgtgggcaa
    10441 aaactccgcg cattttgctg gtctggccga aatatgcgaa ggttacgctg cgcattctcg
    10501 atctcaaagt tttgcagcgt cacgccgatt cgctgggcgc acaattggga ttggtcacgc
    10561 ggcgggcaaa agtgcggcgc gatgccgaat cgctggggat tcccgtcttt gcttcgacga
    10621 cgaccgctca acgcgaactg tggcctgatc ctgttccgag gagcacacgc accccgaaac
    10681 ccccgcgccg cgacctgaga gagatccgcg atgcggtgta ccaaaaagaa cctgcctggc
    10741 gcacatccct gctgggacgt gtgctcactt tcacagtggg ggtcatggcc ttcgtaaccg
    10801 tagcaggcct gttcgtccca cgagccgcgc tgactttata tccagagacc caggttcaat
    10861 ccatcgtcat tcccgtagcg gcaagtgagt ccgctgcttc catttcactt gctggcgcag
    10921 tccccgcgtt gaccttgtct gtgaccgagc aggcagagca atcgcttgcc attgtcagtg
    10981 aaatttctgt gccgaaatcg aaggcaaaag gtgttgtgca gttcacaaat ttgagtcaga
    11041 acgagttgag cattccagca ggcacggtgg ttgcgaccgc cgctgaagtg cctgtaaaat
    11101 ttacaaccct cgaagatgcc accctcgcag caggcattga tgaagttgtg gaaattccca
    11161 tcgaagcaca gcaggcgggc acggatggca atgtgccgaa cgatgctgtg atcgtggtgg
    11221 aagggccgct tgggttgact gtttcggtga ccaaccctga acccgtgaca ggcggtgtga
    11281 tctcgcaagt gacaggcgca aacgacgatg accgcgcaag attgcgcgag gtgatgcttg
    11341 aaaatctgcg ccgcatggcg gagaccgagt tgcgctcgaa gatcggtccc gcagatgttt
    11401 tgctggtgga tacgcttgag atcaaacagg ttctgagcga ggccttcgac ccgccagcag
    11461 gccagcctgg aaaaacgctg gtcttgaata tgcaggtcga attttcggcg cgctatgttt
    11521 cagatgatgc catgagggag ctttcactgg ctacgctcaa tgcggcgggg caggatggat
    11581 tcgtttccac ttcggcgcct gattacaagg tgatcaccga gccctccacc gataattcgg
    11641 gcatcagcca tttcgaattg atggtcacac gcaccctgct gcgtgaagtg gacgaaatgc
    11701 gcgtcttctc catcgtgcgc ggcggcaaac ccgacctgat gaaagcggaa ctggcctcgg
    11761 ccttgtccct gcgccagtca ccagatattt tgctcactcc ctcctggtgg ccgtggatgc
    11821 cgctgatccc gttcaatatt tcggtggaga tgaaatgaac acaaaacagt gcgccatacc
    11881 cagcattcaa cgcctatgag aatcctcgca gttgaccacg gtgaaaaacg gatcggcctc
    11941 gctctgagcg atccgaccgc gaccatcgcc agccctttga aagtcgttga gcatgtttcg
    12001 cgcatgatcg atgccgcaca ggttgcggag atcgccgcgc aaaacgaagt cgggttgatc
    12061 gtcatcggcc agtcttatga cgatgacgga aaccccaacc cggcaggccg acgcgcaggc
    12121 cgttttgcag atgaactcaa aaaacaaacc gaacttccca tcgaaatgtg ggatgaatct
    12181 ttcagcacac aagatgcccg cgccgtccgc atcgaacttg gcctatccag aaaaaagcgc
    12241 ggcggtcatc aggacgaatt tgcagcagtg gccatcctgc aatcgtatct tgaagccaaa
    12301 cggggaagtg gaaatccgta atgagtgaca gccaaatcgt aaatcgaaaa tcgccctcca
    12361 aaaatttcct gcgccgttat caacttcacc tcattcttgc tgttgttgtt ttctgttcgc
    12421 tgacctgtct ttattttgcg ctaggctacg cccccgcgcg cgcttcactg ttgtacggcc
    12481 cgcctgccag cgcgctttcc atttcagacc gcatcgaata ttcggcacgc ctgcttgcgt
    12541 acggcgacac actcaggtca ccgtacgacc cgaacggttt cgaacaatcc ttttttgttg
    12601 agcagggcga atccgttttt tcaatatcca cccgcctcga acaggcaggt ctgatctcga
    12661 atgcgcaagc cttttacgat tacgccgtat atgctggcat tgacctgacg atccagtcgg
    12721 gcgagtacac gctcagcccc gcactttcca tcattgacat cgcccgcgcc ctgcagaatt
    12781 tttccccttc cgatgcaacc cttgtgattc ttcctggctg gcggatggaa gaaattgccg
    12841 cgtccatccc aacctcgggc ctggaatttt cacccgaagc atttctcgcc ctcgcgcaga
    12901 ctccgccccc cgcgcttgaa tttgtcccct catcctccag catggaagga ttcttcttcc
    12961 ccgattcgta taccctgccg cgctccacaa cccccgagca actgctggat gtcgtcgcgc
    13021 ggaatttcgt ccagcacctc accgatgatt tgcggacagg ctttgccaat cagggccttg
    13081 atatccatca ggcggtgacc ctcgcctcca tcgtcgaacg tgaggccatt catcacgaag
    13141 aagctccgct gatcgcttct gtgtacttga accgccttgc catcggcatg aaactcgatg
    13201 ccgacccaac cgtgcaatat gcagtggggt acaacctttt tcaagaaacc tggtggacga
    13261 atcccatcag cgccgccgac ctcgaatttg actcgccttt caatacctat ctgtacattg
    13321 gcttgccgcc tgcgccgatc tccaatccca gcctcgaggc cttgcatgcg gtagcctatc
    13381 ctgaaacctc tgggttttat tacttccgcg ccgcttgtga caactcaggc tatcacgtct
    13441 tcgccgccac acttgaagag caggtcgcca acgggtgtcc gtaaaaatag ccgcgaattt
    13501 cgctttcatt catgaaattc gcggctatga ttttactttt tgaacggcaa tttcaaatac
    13561 gcatcccaat atgaaagtgc aaatttgtca caaccgtcac tgcgctgcaa ccctgtgtaa
    13621 acccaaatgg tggccagctc gcgcacatct gtgggcattc cagcgatcag cttgtcgtaa
    13681 tcaatgcctg taaaagcgat ggcgaattcg aaatggtttt cgattccctt ctgcatcaga
    13741 aaatcaatcc aacgcgcctg atactcttcg ctgaatttgt aatccttgcc gtaggcagaa
    13801 aaatcataag acggtgcgcc cgattcggta atgccgatcg gttttccaaa ggccagtgcg
    13861 ggtgcaaaga aatcatcggg gatcgggtca gccttgccgc tttcgtacat ccaaccgtag
    13921 ggataggcgc tcaatccgag catatccaga tatggcagca gcgtttcgat ctctttcttt
    13981 tgtgtggctt ggtcgccgcc atccaacccg ctcatgtggc tgatcgtgaa tgtggcaaaa
    14041 atgggcaggt cgggatattc cgctttcagg ttttcataca caaattgatg taattgcttg
    14101 taagcctccc aactctcggg tgcgttgacc tgtgccacat tcgcttcgat gccaattgcc
    14161 agataatcgg ggtgaaaata ttccaccaca cggcgcgcat aattcaaata ggccgtcttc
    14221 acatctgcat ggtccagcga gtagccatcc cacggcttgg gcagcggctg gttatcagat
    14281 tcgccccaat atgctgccag cttggagcgg gtcatatcca gcggcgtgat cgaaaccaac
    14341 agagtgtgct ccgctggaat tgcatcgcgc gtgaattgcc aactgctcat caaccctgcg
    14401 ggcaggggag aatcggtcag cgcttcgggc cacggcacac cgccatcaaa atgcagggcg
    14461 gtcatgtcgc cataagtgtt cacgaagttg ttcatgcggt cgattccttc caatgtcgct
    14521 tcgggaggcc agcgcgtgaa acccatgtga aatggacgag attccgcatc caacttaacg
    14581 gcggggctgg ttgctgtaca agcgggtgtc ggcgccgctg atacagtcga ctctccgcag
    14641 gccagtgtgg atagcaatag caagatcaga gcaagcagat tttttatttt catcttattt
    14701 ttttctcctg aaaagaatcc catatttcaa gccatgcatg agcgcaattg ccgcgatcaa
    14761 attgcgtttc atgtgactcc gaaattagtt ttggatgggt ggaaagtgaa ttgaaggatg
    14821 gtctggaaag atcaggttga tcaaaatcgc ccacagaaaa ccacaaagca gcggaagaaa
    14881 aaatgccagc attccgtgta aaagattcgg ttttcgcgca ccacgcccat cagggatcgt
    14941 gtaagcgggc aggtagatca tcaacccgta gacaaagatc cacatggcga attgacttag
    15001 gttctgcgcg ccaaatgcga tggattcacc gatgagtccc tgcaatccca acagcaggaa
    15061 cacccaaaat ggactgaaat cgtagcgctt caaaatccat gcccaggcga agaaccacgg
    15121 gacaaaaacg atcacgctgt gatgcagaat gacatcaaag taattggccg aggcggtgat
    15181 gtaggcttcc ccgattgcca cgccaaagag cggcgcaagg ttggtcatgg tggtggtgat
    15241 ggcttcttcg gtcatggcca gtaaaatgcc aaaaagaatg aatttgacct gccaattcag
    15301 cggaatgccc tgaacgaagg cttttatcct gtttcggaac agaaccatac ccgagccgcc
    15361 cagcaccacc caaaataaaa gcagacccga ccccattgaa ataatggcat gaagtacacg
    15421 gtcgctccgc gtggcaaaaa agaccagaag ccaggttagg gcaaggtatg ctcccagtag
    15481 aaaaataaaa cctgttttca agcgttgtga aatggacata tctatcactg atacaggcca
    15541 aatgccgaat ttgttgcagg aatcaaaaac gggattcgat ctgtttcgaa tcccgttttc
    15601 tggttttggt atgtgggctg gagttcgagt tctttagcgc atttgacttg cttttacaaa
    15661 atacccgacc agcagcatca ccagaccgat ggctgacaaa ctcaatccct gccagagtac
    15721 ggggctgagc aatgcctgca gcatggcagt ggcaaggtca ctggcgtagg ccaggaaaat
    15781 ggatggaaga taatcggata atctggaggc cagcatgcct tgaaaaacaa aactgaaaat
    15841 gggggcgccc gtgaagctca tcaaacctgc aaacgcgcct gtcgccaaaa aggaagcacc
    15901 ccaccaagtc aaccaactgg tgaaggagtt gaccgcaaag atcgtcatca agagcaggaa
    15961 gcccagcggc aaaatggggc tgagccgcat gaataaccga aaggtctgca gtttctggcg
    16021 tggatcattt tgaagcggcg cttcgtacac ggtgatctgg tcgggaatga gcgcagccgc
    16081 cacttgtagt tgtgcctgaa tggctggcgt catcacgggt tccaactcgg cgggtgggtt
    16141 gcaaaattgg atgctcccgc ccgagatcag gtcaatgccc atttgggcaa gctgcatcag
    16201 ggtgcagctg ggttgggtgc tcaaaagccc gtaaatggcc tgcacgcccg aagggccgac
    16261 catgcttgtc tttagcggag caagcgaaag ctggacaata tccgattgtt gattcaagta
    16321 ggtgaaggtc gagtttaata gtccatctcc catggttgta agtgcatcgg gtgcgagtat
    16381 ggtgcggaaa aatgcatccc aggcttgcac atccattcgg ctcatcacaa tggggagttg
    16441 attctgatcg gtggtgacca tggcttcagc gaggaccgtg gggagtctgt tgtaaaaatc
    16501 atcttttgca aaggctttgc ggtaggtttc ggctgaaaag ccctcacgct caaaattaaa
    16561 aagcagcaac gcgaaaaccg cgctcatcac aaagagcacc gcaaaaagac ctgccagaga
    16621 ttttttgaaa gtttccatat tttatttttg attacataga caaacggcag accacaatcc
    16681 gtggcctgcc gtcagacatg caattcctta gctggctgtc aaaatttcga tgtagtcttg
    16741 cgtgactact tctgcgtggc ctgcgccgag caggtcaagt aattcgctga ccaattcagc
    16801 cttgaggtcg gcgtctttgc gactccacgt gcggcttttg atttcaagga agtgccccat
    16861 gggcggctcc ttgatttcat cgagattgat gaatagttcg gtgtttttat agcggatgtg
    16921 ccagcgcagg cggtcttttg tgatggagat ttcctttgca ggtttgaaat actcacgata
    16981 aaaacgcgct gtgtgaatgg caggcgcaat gaagcggctg cgcgaaagca gcacatcatg
    17041 gccgatctcg ccttcgcgtt tttgcccgag cagggtcagg cgcgagcgga cattttgcga
    17101 attccctttt tcgtctatga ggttgtcttc gcggaagcgc agacgtccct gggatggatc
    17161 gtcgaagagg aagtaatcgt cgaactgctg gtaatgcttc tgggcggtga tctcgatctc
    17221 ggggcgcttc attgcctgga ccaatttgtc tggttctgcg atcttgacct tgacctgcac
    17281 ctcgaaagat tcctcctgca tcgagccgcc aagcgcgatc aatttcgaaa gcaccgattt
    17341 ctcacgttcg atcaggaagg tatcgatctt ctttgcaaca ccctgggcct ctttgatcaa
    17401 gtctgcttcg ttgagggtga gcaggttgtg gtcacgcatc aaccacttgc cgttgaccat
    17461 cacatcggtc acatcagtgg atttggaggc gtagaccagt tgggcgtagg cattgtgcgg
    17521 gtcacgacga aaacgcgggg agttatgcgc gggggatgta tcgacgataa tcaggtcggc
    17581 gcgtttgcct gcttccagcg accctgtcaa atgtccgatg tgaagcgcct gagccccgag
    17641 gcgggtaccc atcagtaatg cagtggccgc cgggacgacg gtggggtcat tcgaagaagc
    17701 cttcgccaca aaagaagcga ggcggatctc ttcgaacatg tcgaggtcgt tgttggaggc
    17761 ggggccgtcg gtgccgatgc ctacattgag gccgacatcg agcatttttt gcacgggtgc
    17821 aaaccccgaa gcaagtttga gattcgaaga tgggttgtgt gagatgcccg catttacatg
    17881 ctggagtgtt ttcatttcgc caacatcaat gtggacacaa tgcgcggcga taaccttcgc
    17941 ttcaagcagt ccctgctttt tcacataagg cacgacgggc atgccctgtt cgttgcgcat
    18001 gttttccact tcgaatgcgg tttctgaaat atggatgtgc agcggcacat cgtattcttt
    18061 tgcaatgtcg gtgcaggtgc gtaaaatttc gggggtggtg gagtaggggg cgtgcggcgc
    18121 aatggctggg acgatgagcg ggtgtccctt ccaggtcttt ataaattcac gcgcatatgc
    18181 aatggaatct tcaaaagcgg gcgcatcggg tgcgggatat ttcatcaccg attggccgca
    18241 aactgcgcgc atgcctgctt cggctgtggc ctgtgcgatg tccgattcaa agaagtacat
    18301 gtcgttgaat gtggttacgc cgctgcggat ctgttcggcg caggccaatt gggtgccgag
    18361 acgcacaaat tcaggcgaaa caaattcacg ttccacgggc agcatgta
//
LOCUS       DAOQLC010000088        18406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1057_length_18406_cov_24.023541, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000088 DAOQLC010000000
VERSION     DAOQLC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1057_length_18406_cov_24.023541"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PL000_14710"
     CDS             <1..1110
                     /locus_tag="PL000_14710"
                     /inference="COORDINATES: protein motif:HMM:NF021539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HNC90187.1"
                     /translation="GSGFFIQKENPMAVKKSTKAKSKPKQEEPIPVEEPVWTYRGYRL
                     KTSEFVTAMVHFFRAEIQRANVWRTRLDTTTNWAVVVTGATLSIAFSQSNVHHSVVLL
                     NTLLVLWFLFIEARRYRYYELWSYRVRLMETDFYAAMLVPPFHPSPEWAENLAENLLT
                     PSFPISMWEAFGRRLRRNYYWIFLILLASWVAKIYLFPEAPASMSEFVVRSSVGPVPG
                     EVMIGLGISFYTFLAIFAIATINMTKATGEILPRFGDESAPAAPSMEGKHTGLRALLV
                     PRRRRRQLLALIITDKADSVSGRIMTDLKRGVTSMLGKGMYTGKDRSILMCALTITEV
                     HNLKSAVAKEDPKAVVIVSPAQEVFGGGFAPLEEK"
     gene            1107..1718
                     /locus_tag="PL000_14715"
     CDS             1107..1718
                     /locus_tag="PL000_14715"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNC90188.1"
                     /translation="MMLAEILCESSRHHDHLCPRQVLGVRMGLYAGELLGLQLPRADK
                     RLLVIAETDGCAVDGISAATGCRVGNRTLRVMDFGKVAATFIDVSTETCIRIAPSVQA
                     RSLALAYSPNSTSRWDAMLSAYQSMPVMDLLAFQRVQLDTPLAQILSVPGKKVQCEVC
                     GEEIMNEREVLKNEMILCRSCAGDGYYRYPVDSLSVEQLEQNG"
     gene            1900..3036
                     /locus_tag="PL000_14720"
     CDS             1900..3036
                     /locus_tag="PL000_14720"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNC90189.1"
                     /translation="MKILFLGTHGQFNIGDELLLETFLAQLGIEHQYAVNSYDPTFTQ
                     NAMRPKFKVESFHTTRELPRFLKYLLTSDLLFFGGGSIIKELYASVGRNPYSTLLMIL
                     ATVTFAKQIARKRVVMSNIGVGPIMTPRGEQLARWILNQVDILSVRDEKSLQTALKLG
                     LAPSRVMLVPDAVFANPPEVFVSHPEIPPPSDKTRIALNLNYDIENPNAWEAFLTNLA
                     TALKQLGETHALEIHALPMQSKFKANDDLSVLKDFTKRIPDIPCILHDPQTAQEAAQI
                     IQGMDMVLAERLHTLVISSVLGKPFFGLVYDVKVKELIDYLGMSEHSLNINQSFTAEE
                     LVNGINCVLDQRETISTHLLKRSTSLREQLGKYFDEIRQKFIGR"
     gene            3005..>5178
                     /locus_tag="PL000_14725"
     CDS             3005..>5178
                     /locus_tag="PL000_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90190.1"
                     /translation="MKSARNLSAADDQPGISEKQPRFALLSFRALTIRQISMIVGICY
                     FLISFFVFRHVIANIPLVLSGQAVINGDELVPFFNPNSQLIDQAAGKFNQLTNGYEFR
                     VRYSILTTWFRYYKVLPFAIVLVIPTITYLAYLATASLLRKALPQFKPENIYQLTAAP
                     ILMIFLIMMYAKITHFYTLILGFSLCLISITLMTYGLVFPQPQPYKPILAACVITLFN
                     PAVHYLILFALFMAMTVFTLIVMDGIAAIKNGSWRNIYRPRVWWGGVKTLWKDWRRLF
                     VEVRFWRCVGAFVMLGVGTLLPYGLFVKFYALRGVPNLSETVPADFYFIQDASISLGH
                     VLALDMAGIMDKELTGDYLAKVPRWSNMIYTLLMFIPLVVKRARDDIFHSEPLKALSA
                     VLYANTIFTMWATLGYSTAPWVPTFHRTIAFISLKANGLQSGIGDLVVKLMGTIVQVL
                     RFPHRFELVTFMMACILVSISMIWMHHVFMEKGFGELVWVLTGKRQGAAKKSLKQNQA
                     RAGAEAQRFIPLTMVLLFMVPLFLNSSYRLVFFSGDFNEFLTPYPVEPLKEVKEALLQ
                     LPPGKVVVLPPTETAKVIVDINGVEHKFIDKFHIYYLDLPSYYYGLTGDSDNKHEFFL
                     LLRALYYQQPWWINIARDLNLRYIVVNKELVANTVGGQEYLREVEKILIPELDRRDAY
                     LKKLMENESFVLYEFTDLPKAERVPLYLDVDWNKFIQILSSNL"
     assembly_gap    5179..5278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5279..5722
                     /locus_tag="PL000_14730"
     CDS             <5279..5722
                     /locus_tag="PL000_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90191.1"
                     /translation="EYRLLMRGAISAVDLEMTSKLFETPQRITLASDPSNLAFFDKRM
                     VFSSKRSPLDVSAYTLEELGLLIPSDVVVVNYQYQFFDLGTVTAGMGKYPIYFTKLND
                     APMLVEGILLVPEEAYRSMSLPPNVTVVPANELCCGSVIIQGEEP"
     gene            5844..8366
                     /locus_tag="PL000_14735"
     CDS             5844..8366
                     /locus_tag="PL000_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90192.1"
                     /translation="MKTFVYPYIEKIKGLQERLTAILDRLRTKIPGLPSNRILISAAG
                     LVFVAVFIFAALQTLLAPEQPVTTYTVPYTQEFDEVNLKRWFSDAGVWTIRESTLLQT
                     VGGEDAGQLHIPLTVPEDQPYHASVFITLKKDSKNVGLSFNAQYPNLLEEQHRVYISR
                     INPDTLELVAGYMDETGAFVTQAQVPLSINTTEFRLDLYVYSNTYLVQVNGQRLIENR
                     PLFYKNGMIGFYALGPVIFDTYKLTAAENQNPGDMIYTSDFDQDPGGAGWVPISGMWQ
                     IASKQMVQSDSAVNDAAIGYETSTFENYTLQTTFNHLSGQGAGVLFNMPSPYQINGAH
                     LVRYSDETDSLVWGYYDAKGVFNRQGFADVSAAGKAAHTLQIFSGDNSYDIFLDDEML
                     ARDVPLESIQGSVGLTTSRSSAGFTTVEVFPLFGTTASPVAALTQPSKNTPLPKASPT
                     QVLPTQASPTQTPAPEKTPTANADSVTPIATGDKNIVNGGSAPYHGVFDGKLSDAGWK
                     ILNGDWKFSNGNFVQTKVEGFDFATVYTKTAFKSFSIQIGLMHVEGSGGGIIFNLPYA
                     DRLNGATMVRYSDKRDNAVIWGYFDENGVYQNQGYADVPHAGTDRHTLRVVAGEASYG
                     IYVDERLIVQAVPYRTKQNFGYFGLLTSRSSVVYDEVLVDGVGAVFKGTYSNMDGFTD
                     QRVVSGKWNIETNKASQTVTDVSDYVWNTGVQASDYTVTSKITLPNTSDSGGGFIIHM
                     TERGTKNNSYIVRLKDGGKAIWWGTINAEGKFKGQGSAPLEKTSNTFVLKLVVKLDSM
                     TIYVNDKEIVKNVTLSSQEGWIGLLAYGGPVIFEDVKLDVTQ"
     gene            8363..9529
                     /locus_tag="PL000_14740"
     CDS             8363..9529
                     /locus_tag="PL000_14740"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HNC90193.1"
                     /translation="MKKLPAWLLIPLLAFSLSRLLIFGAGILADTMLPTEEGHWIADE
                     NSPFLSMWTKWDSQYYVDIATDGYWFRPKQQSNVAFFPLYPMLMRVTSKLFGGNLILT
                     GVLLSNLAFLGALIFFYLLTEREFDSDSARRAVFYLAFFPTSFFFSSLYTESLFVLLS
                     VATMYYARTQRWVPATILGMLTAATRNLGILMWALVLWEWLRVQGWSFTAMHKKQTWL
                     NLWNGFKHNWFQVIIISLIPLGMLAYIYFLQVNFQRPLAFIETQAAWGRENIGPIAVL
                     QKNITALMEGEVNKGWLTRFWNVTTMLFFLALVPFIWFRLGEGYAIFVLIMLLVPSAS
                     AVGSIIRYVLTQFPAFMLMGWWGRREAVDRTFGMSFAVLLGVFVSIFVNWIFVA"
     gene            9542..10306
                     /locus_tag="PL000_14745"
     CDS             9542..10306
                     /locus_tag="PL000_14745"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC90194.1"
                     /translation="MPYENYAQWKIVPISEEPKLSIVIPAYNERERIVATIGAIASHV
                     SDLGFPWEMFIADDGSKDDTVAIVEELGLVNLTVLKAPKNGGKGSAVQRGMLAARGKY
                     VLFADADNSTPIEEVSKLLNKLEHDGFDVAVGSRAAEGAEEGKKSLFRKILSGGLRWI
                     VKNIFRIGVRDTQCGFKMYTHEAAQKLHAKQTIMGFSFDLEILYLASKYGYKIAEVPV
                     SWIDAPGSKVDTRKEVQRFLRDLLKIKSNDLRGVYQ"
     gene            10303..11508
                     /locus_tag="PL000_14750"
     CDS             10303..11508
                     /locus_tag="PL000_14750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90195.1"
                     /translation="MTTTNQMRIAIVTTHPPSKGSLNEYAFHFVRFLKTKVEYVQEII
                     LLVDELPESESYSLGQKQDEVPIRCVPCWKFGALDNILRIRKAVKEVEPDIVLFNIQF
                     ASFADKKIPATLGLLTPWFLKVSGFKVAVLLHNIMETVNLKSAGFGSNPLIEAVIRLM
                     GNIVTRFLLAADLVAVTIPKYVEILESKYDATNVLLAPHGSFEEPTDTEPKPLPGGTP
                     TIMTFGKFGTYKTVDLLIESHAMLQTQHPDLRLVIAGTDSPNAAGYLKTMQEKYAHVK
                     NVEYTGYVAEEDVPRIFGEAWVVAFPYTSTTGSSGVLHQAGGFGKAVVLPKLGDLAEL
                     VLEEGYDGEFFEPTNSKSLADAIARVIDNPERQMEIGLRNYTAANGLPITEVVDWYLI
                     HFEELLKRK"
     gene            11518..11850
                     /locus_tag="PL000_14755"
     CDS             11518..11850
                     /locus_tag="PL000_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC90196.1"
                     /translation="MEMIKVVNGPISVLQLKGRFDAHEVEPVNAWLQEQVNQGRVKVV
                     VNLEGVNFIDSTALSVLVRGMKNCREKSGNLNLCSLQQPVRVIFELTRLDKAFGIYAS
                     ETEAGKAF"
     gene            11857..13767
                     /locus_tag="PL000_14760"
     CDS             11857..13767
                     /locus_tag="PL000_14760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNC90197.1"
                     /translation="MLAEHLENHRKKFYSLASVWMRVGATCVELMEAGQVLATFPQEC
                     SDSSPALFVHARTSNLALRIHGIVDENCRPIAESMLDILSNLLTADLELENLTAALVE
                     TQDRLVAIYELSQASRRTLEIPALLDLLVQESTHLMNVDGGFAVLMEKGKKSVIHQVS
                     DAPLHHAHLEAAATLFRRDPTRHTFKESETLPPGLRNVMMVSLPVRDEVFAAFGVFNK
                     TGDFTSPDIKLAKAIAGYVGARLESAFLHKEALERARMETEMDIARQVQIAILPQSLP
                     KVTGLDIYATSTPALEVGGDFFDVIDRSEDSLVFAVGDVTGKGMPAALLMSMTHTVIK
                     SASRKMPFSHPHQVLNRLNHDLFSDFSNVGMFSTVFLGLLDRKNCTVTFSNAGHSPIF
                     YIPAGCEAHLLEAQDIPIGVLDIYDYTSQTIKFAKGDIFVIASDGFPESRNSSDEMYG
                     YDRMKACLERSRNAPAEEMVSALLADVNAFSGSHLQDDDRTILVIKVNELRMQSELIN
                     IRSTYKEIRLPSERLRGLLAYNQVTEEIINGCELALHELLTNLVDHAYEGNENGNITV
                     NITCDTDFVTIETRDEGKPANVDMAGIAMPDPTDLAEGGYGMAIIQMLMDDVKYKFED
                     SKNVWRLIKRIQ"
     gene            13794..14138
                     /locus_tag="PL000_14765"
     CDS             13794..14138
                     /locus_tag="PL000_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC90198.1"
                     /translation="MTLEISISEQVIKIATIALNGRLDALEAPALRKRVDDYLANEFS
                     HFVFDLSDVAVLDSAGLAVLVSALKRARLKNGDVRLVWPKAEVASRILKLTKFDQVFV
                     SIERSEIVPRGF"
     gene            14140..14505
                     /locus_tag="PL000_14770"
     CDS             14140..14505
                     /locus_tag="PL000_14770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90199.1"
                     /translation="MARILIVDDEPNNQRILNYTLNKAGYETITAANGETALELLDQY
                     DADLAIVDVAMPVMDGLTLLKHIRSADKFEHLPVIILTGSGDDEERLRAEREGIQGFL
                     TKPSSSKMVLETVQNILKR"
     gene            14502..15788
                     /locus_tag="PL000_14775"
     CDS             14502..15788
                     /locus_tag="PL000_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNC90200.1"
                     /translation="MIQKIKELLRSKIASASALLMVSMTVVNAGNYVYNLIMGRWLGP
                     SLFADLSLIVTLLLIVTFITAPIQMTTARYTAIHAADADVKTLASIRRFIWRIAAALG
                     LTLTVLFATFAPLLQSFFHTQSFLPFIIFGAALPFSLLQGVERGILQGRTHFKVLAIS
                     YQVEMWTRLFVSIILVALGLSVNGAVIGISLSFFATWLTARLAVRDLFTKEDIQTELR
                     KELFAFAVPVLVSQLGQILINNSDVILVKRFYESDQAGQYAALALIGRIVFFATWSVV
                     TTMFPIVAQKQKQGEPHSHLLWVSLGIVLGVSLPIVLLTLFVPEWIVKILFGSQYLGI
                     APLLWLYALATTLYALANVVINYRLSLGMGRETRFALAAGVAQVLGIIFFHQTLAQVV
                     YVQVVVMAVLFASLLWWNFSGRARQLPLIQVAKSNA"
     gene            15781..>18406
                     /locus_tag="PL000_14780"
     CDS             15781..>18406
                     /locus_tag="PL000_14780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC90201.1"
                     /translation="MLDLFFSVAMDALIAFDESGRVIRANQFAAEMFGYSMDEFRTMT
                     VENLLPKKNAKNRKDLVLLLLNTNVENRKIVKSHRVWVLHKSGYEFLVMASIGKGSLN
                     DQPVSLISLRDSLIDGQEENLTDLPITDARENPNAEIRIGRDGRILFANKSGKRLLRR
                     SGMVVTGQVPSEWVGFFEQALKRKTHIQRVIQHGRRLYSCHFNPTPEMGYVTLYALDI
                     TEYEAEKNRLALSDEILRSIGNLVLVANSNAEVVYISPSVKEILGYEPEELIGNGWWE
                     LERISGGDVLAERDYISKAAAGLRQVDDKPYEHRVRHKNGDWRWLMLADRKGPRDLLI
                     GIGTDVTESKKTGGELQNQRDFAQTLTTQMGQGLTVIDEEGHFTFANPYSAKILGYET
                     EQLAGLTAFDVTFPEDHELVKTVLSKRNVGEVTTLEIRLRGRNGDEVYALVTSVPHVV
                     DGQFAGTINVITDLTERRQMENLLRDYATAIEKSNIDLADARDRALEASYLKSSFLAT
                     MSHEIRTPMNAIMGMTEMLLSTDLNDEQREFAQTIDSSTQGLLAILNDILDFSKIEAG
                     KLSIIPSVFNPVEMTRDLIKLFQPRAQEKNITLSMMATASIPETLVGDGGRIRQILGN
                     FVSNAIKFTEKNGCVFINISGTSINQNTLMTTFTVQDNGIGVSDMMRSKLFEPFTQAD
                     GSNTRKHGGTGLGLAISRRLVDLMHGEIGFESFEGTGSTFWFSLPLYTTAPKDQEETE
                     FSSRGMQKKYLNYSSRKPILIVEDNLVNRDLFTLQLQEFDLTIKQAGNGREAVELLQI
                     HPDDFSLVLMDVHMPEMDGFTATRLIRKSEAGTGRHIPIVAITADAMVSSKKLCLEAG
                     MDDFLTKPVTLANLDKLFR"
BASE COUNT         4344 a         4299 c         4783 g         4880 t
ORIGIN      
        1 ggaagcgggt tcttcatcca gaaagaaaat ccaatggcag taaaaaagtc aacaaaagcg
       61 aaatcaaagc cgaagcagga agaacccatc ccagttgaag agcctgtttg gacgtatcgt
      121 gggtatcgac tcaaaaccag tgagtttgtt acggcaatgg tacatttctt ccgcgccgaa
      181 attcagcgtg ccaatgtttg gcgtacgcgc ctggatacga ccacgaattg ggcggtggtt
      241 gtaacgggtg cgacgctttc gattgcgttt agccagtcga acgtacatca ctcggtggtt
      301 ttgttaaata cgcttttggt tttgtggttt ttgtttatcg aggcaagacg atatcgctat
      361 tatgaattat ggagttaccg tgtgcggtta atggaaacgg atttttacgc cgccatgctc
      421 gtgccgccgt ttcatccctc gccagagtgg gcggagaacc ttgccgagaa tttgcttacg
      481 ccaagttttc ccatctccat gtgggaggca tttggcaggc gtttgcgcag aaattattac
      541 tggatctttt tgatcctgct ggcttcatgg gtggcgaaga tctatctttt ccctgaagct
      601 ccagcaagca tgtctgagtt tgtggtgcgt tcgtctgttg ggcctgtacc tggtgaggtt
      661 atgatcggtt tgggtatttc cttttatacc tttctcgcga tctttgcgat tgccacgatc
      721 aatatgacca aggcaactgg tgaaatcctt ccgcgttttg gcgatgagtc tgcaccggcg
      781 gcgccatcga tggaaggaaa gcatactggt ttgcgcgctt tgctcgtgcc gcgccgaaga
      841 aggcgacagt tgcttgctct tatcattaca gataaggccg actctgtatc aggtcgaatt
      901 atgaccgatc tcaaacgcgg agtaacctcc atgctgggca agggaatgta cacaggcaag
      961 gatcgttcca ttttgatgtg tgcattgacc attaccgaag tgcataattt gaaatctgca
     1021 gtggcaaagg aagacccgaa ggcggtggtc attgtttcgc ctgcgcagga agtgttcggt
     1081 gggggatttg ctccgttgga agaaaaatga tgcttgcaga gattttgtgc gaaagttcgc
     1141 gccatcacga ccatctttgt ccgcgccagg tattgggtgt gcgtatgggg ttgtatgcgg
     1201 gggaacttct tgggttgcag cttccacgcg ctgataaacg tctgttggtg atcgcagaaa
     1261 cagacggctg tgcggtggat ggaatttctg ctgccacggg gtgccgcgtt ggcaaccgta
     1321 cgcttcgtgt gatggatttt ggaaaggttg cggctacctt tattgatgtc agcactgaaa
     1381 catgcatccg aattgcgcca tctgtacagg cacggtccct tgcacttgca tattctccaa
     1441 actcaaccag ccgttgggat gcgatgttgt cggcatatca atccatgccg gtgatggatc
     1501 tgcttgcatt ccagcgggtg caattggata ctccacttgc gcagatcttg agtgtgccag
     1561 gtaaaaaggt gcaatgcgag gtttgtggag aggagatcat gaacgagcgt gaggtcttga
     1621 aaaatgaaat gatcctgtgc cgttcttgtg cgggggatgg atattatcgc tatcctgttg
     1681 actcactttc cgttgaacag cttgaacaaa atggataaac aagatcttaa gtgagtgcta
     1741 cttaagtttt ttcaaggctg cagaaactct gcagcctttt tgtttatgcc aattcaaaat
     1801 gccttgcaaa ttggcaagtt ttttgcgttt gcaatcatcc tgtcagattt tgtttttgtg
     1861 aaagtgatta ttatgaattc aaccacaaag gcggacccaa tgaagatact ttttcttggc
     1921 acacatgggc agttcaatat tggcgatgaa ctattgctgg aaacattcct tgcccaactc
     1981 ggtattgaac atcaatatgc cgttaattcc tacgacccaa ccttcacgca aaatgccatg
     2041 cgcccaaaat tcaaagttga gtcattccac accacgcgcg agcttccgcg ttttttgaaa
     2101 tacctgctca ccagtgacct tttgtttttc ggcggcggca gcatcatcaa ggaactctat
     2161 gccagcgtgg ggcgcaatcc atactccaca cttttgatga ttcttgccac ggttacattt
     2221 gcaaagcaga tcgcacgcaa gcgggtggtg atgagcaata ttggagtggg gccgatcatg
     2281 accccgcgcg gcgaacagct tgcccgctgg atattgaatc aagtggacat tctttccgtc
     2341 cgtgacgaaa agtcgcttca aaccgcgctg aaacttggtc tggccccgag cagggtaatg
     2401 ctcgtccctg acgctgtttt tgccaacccg cctgaggtct ttgtctctca ccctgaaatt
     2461 ccacccccat ccgataagac ccgcatagcc ctcaacttga actacgatat tgaaaatcca
     2521 aatgcatggg aagcctttct cacgaatctc gctactgcgt tgaaacaatt gggtgaaact
     2581 cacgcgctgg agattcatgc gctgcccatg caaagcaaat tcaaagccaa tgacgatctc
     2641 tccgtgctaa aagatttcac aaagcgcatt cctgatattc cctgtattct ccatgacccg
     2701 caaacggcac aggaggctgc ccagatcatt caaggcatgg acatggttct tgcggaacgc
     2761 ctgcatactc tggtaatttc atccgttttg ggaaaaccat ttttcggttt ggtctacgat
     2821 gtcaaagtga aggagttgat cgattacctt ggcatgtcgg agcattcact gaatatcaac
     2881 cagtctttca ccgcagaaga actggttaac ggaattaact gtgtcttgga tcaacgtgaa
     2941 acgatcagca ctcatctgtt gaagagaagc accagccttc gtgagcaatt gggaaagtat
     3001 ttcgatgaaa tccgccagaa atttatcggc cgctgacgac cagccaggca tttctgaaaa
     3061 acaaccgcga tttgcgctgc tttcatttcg tgctctaacg attcggcaga tttccatgat
     3121 tgtgggaatc tgctattttt tgatttcttt ctttgttttt cgccatgtca ttgccaatat
     3181 tcccctcgtt ctaagtggac aggccgtgat caacggtgat gagttggtgc cgttcttcaa
     3241 cccaaacagc cagttgatcg atcaggcggc agggaaattc aaccagttga ccaatggcta
     3301 cgaattccgt gtccgatatt ccattttgac cacctggttc cgatattaca aggttttgcc
     3361 ctttgccatt gttctggtca ttcccacgat cacatatctg gcataccttg caactgcttc
     3421 gcttttgagg aaagccctgc cgcagttcaa gcctgaaaat atctatcaat tgaccgccgc
     3481 acccatcttg atgatctttt tgatcatgat gtatgcgaag atcactcatt tctacaccct
     3541 gatcctggga ttttcccttt gcctgatctc gatcacgctg atgacctatg gcttggtctt
     3601 tcctcaacca cagccatata aaccgatcct ggcggcctgt gtcattacac ttttcaatcc
     3661 tgcagtacat tacctgatcc tttttgcgct tttcatggca atgaccgtct ttacattgat
     3721 cgtcatggat gggattgcgg cgatcaaaaa tggttcgtgg cgcaatatct atcgtccgcg
     3781 ggtgtggtgg ggcggagtga agactttgtg gaaggattgg cgcaggctgt ttgtggaagt
     3841 ccgcttttgg cggtgtgtcg gcgcctttgt catgctgggg gtggggacac tgctgccata
     3901 cggcttgttt gtgaagttct atgccctgcg tggcgtgccg aatctttctg aaacagtacc
     3961 cgccgatttt tatttcattc aggatgcatc catttcattg gggcatgtct tggcgctgga
     4021 tatggcaggt attatggaca aggaactgac aggtgattat cttgccaaag ttccgcgctg
     4081 gtcgaacatg atctatacgt tgttgatgtt cattccgctg gtggtgaaac gcgcccgcga
     4141 tgacatcttc cattcagaac cgttgaaggc tctttcggct gttttatatg ccaatacgat
     4201 ctttacgatg tgggctacgc tgggttattc cactgcgccg tgggtgccga cctttcaccg
     4261 taccattgca ttcatcagcc tgaaagccaa tgggctgcag tcgggcattg gcgatctggt
     4321 ggtaaagctg atggggacga tcgtgcaggt gctgcgtttt ccgcaccgtt ttgaactggt
     4381 cacattcatg atggcctgta tattggtttc gatcagcatg atttggatgc atcatgtttt
     4441 tatggaaaaa gggttcggtg aattggtttg ggtgttgacg gggaaaagac aaggggcggc
     4501 gaaaaaatcc ttgaaacaga atcaggcccg ggcgggggct gaagcgcagc gtttcatccc
     4561 attgaccatg gttttgcttt tcatggtccc gctgttcttg aattcatcgt atcgcctggt
     4621 tttcttttca ggtgatttca acgaatttct aacgccatat ccggtggagc ctttgaaaga
     4681 agtgaaggag gctttgctgc aattgccgcc tggcaaggtg gtggtattgc cgcccactga
     4741 gactgccaaa gtgatcgtgg atataaacgg cgttgaacat aagttcatcg ataaattcca
     4801 tatctattat cttgacctgc caagttatta ctatggtttg acgggtgatt cggataacaa
     4861 gcatgaattc tttttgttgc tgcgtgcctt gtattaccag cagccgtggt ggatcaatat
     4921 tgcgcgtgac ttgaaccttc gttatatcgt agtgaacaag gaattggtag ccaacaccgt
     4981 gggcgggcag gaatatctgc gtgaagtgga aaagatcctg atccctgaat tggatcggcg
     5041 cgatgcgtat ttgaaaaagt tgatggaaaa cgaaagcttt gtgctgtatg aatttaccga
     5101 tctgccaaag gcggagcgtg tgcctttgta tttggatgtc gattggaaca agttcattca
     5161 gatcctttct tcgaacctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     5281 ataccgtttg ttgatgcgtg gtgcgatctc ggctgtggat ttggaaatga cctcgaaatt
     5341 gttcgaaaca ccccagcgga tcactctggc ttcagacccg tcgaatttgg cctttttcga
     5401 taagcgcatg gtattttcgt ccaaacgcag cccattggat gtgagcgcgt acacattgga
     5461 ggaacttggc ctgctgattc cttcggatgt ggtggtggtg aactatcaat atcagttttt
     5521 cgatctgggc actgttacgg ctggtatggg gaaatacccg atctatttta caaaactcaa
     5581 cgatgcgccc atgttggtgg aggggattct gctggttcca gaggaggctt accgctccat
     5641 gtccctgccg cctaatgtaa cggtggtgcc tgcaaacgag ctctgctgcg ggtcggtcat
     5701 cattcagggc gaagaaccat gagagattta ggcataaaaa aaatgttaat tgtgttttca
     5761 aaacagcaag ttttttaaaa atgactctga aacgcatttt caaaagtggt ggacttagaa
     5821 tggaaaagaa aaacaaataa acgatgaaaa cttttgtgta cccttacatt gaaaaaataa
     5881 agggccttca agaaaggtta acagccattc tcgacaggct gcggacaaag attcccggct
     5941 tgccatccaa tcgtattttg atttcagctg ccgggcttgt ttttgtggcc gtttttattt
     6001 ttgccgcctt gcaaaccctg cttgcaccgg agcaacccgt cactacttat acggttccct
     6061 acacccagga attcgacgaa gtgaacctca aacgctggtt ttccgatgcc ggggtgtgga
     6121 cgattcgcga aagcacactt ctgcagacgg ttggtgggga ggacgctgga cagttgcata
     6181 ttccgcttac tgtgccggaa gaccagccct accatgccag tgtgtttatt acgctcaaaa
     6241 aggacagcaa gaatgtcggg ttatctttta acgcacaata tcccaacctg cttgaagaac
     6301 agcatcgtgt gtatatcagc cgcatcaacc cggatacgct cgaactcgtt gctggttaca
     6361 tggatgagac cggtgccttc gtaacccagg ctcaggttcc actcagcatc aataccaccg
     6421 agtttcgact ggacctgtat gtctatagca atacttatct tgttcaggtc aacggccagc
     6481 ggctgatcga aaaccgcccg ttattttata agaacggcat gatcggtttt tatgcgctgg
     6541 gtccggttat ttttgatacc tacaagttga ccgctgccga aaatcaaaac cccggcgaca
     6601 tgatctatac cagcgatttt gaccaagatc cgggcggggc gggttgggtt cccatcagtg
     6661 gcatgtggca gatcgcctcg aagcaaatgg ttcaaagcga ttcagcggtg aacgatgccg
     6721 ccattgggta tgaaaccagt acttttgaaa attacaccct gcagaccacc tttaatcatc
     6781 tttccgggca gggagcgggt gtactattta acatgccctc gccgtatcaa attaacgggg
     6841 cacatcttgt tcgatattca gatgagaccg attcactcgt ttggggatat tacgacgcga
     6901 aaggtgtgtt caaccgtcag ggatttgccg acgtttcagc agcagggaag gctgcccaca
     6961 ccctgcaaat attctccggt gataacagtt acgacatctt tctggacgat gaaatgttgg
     7021 cgcgcgatgt gcctcttgaa agcattcaag gctcggtcgg tctgaccaca tcccgttctt
     7081 cggctggctt taccaccgtt gaagtgtttc cgctgtttgg taccacggcc agccctgttg
     7141 ccgcactgac ccagccttca aaaaatactc cgcttccaaa ggcatcacct acacaagttt
     7201 taccaacaca agcctcaccc acccaaaccc cggctcctga aaaaacaccc accgcgaatg
     7261 cggattccgt cacaccaatt gccacgggcg acaagaacat tgtcaatggt ggcagcgctc
     7321 cctatcatgg tgtatttgac ggcaagctgt cggatgctgg ttggaagatc ctcaatggag
     7381 attggaaatt ctcaaatggt aatttcgtgc agaccaaggt ggagggcttt gattttgcca
     7441 cggtatatac caaaactgcc tttaagagtt ttagtattca aattggtctg atgcatgtgg
     7501 aaggaagcgg cgggggcatc atcttcaacc tgccttatgc agaccgtttg aatggcgcca
     7561 ccatggtgcg gtattcagat aaacgcgata atgccgtgat ctggggctat ttcgatgaga
     7621 atggcgttta tcaaaatcaa ggatatgccg atgttccaca tgcaggcacc gaccgccata
     7681 cccttcgtgt ggtggcaggc gaagcctctt atggcattta tgtggatgaa cggctcattg
     7741 tgcaagcggt accctacaga acaaaacaga actttggtta ttttggtctg ctgacctccc
     7801 gttccagtgt agtgtatgac gaagtgttgg tggatggggt tggcgcagta ttcaagggta
     7861 cttacagcaa catggacggt tttaccgacc agcgtgttgt cagtggcaag tggaacattg
     7921 aaaccaacaa ggcatctcaa accgtcactg atgtttcaga ctatgtctgg aatacaggcg
     7981 tacaggcttc cgattacacg gtgacctcaa agatcacttt acccaacacc agtgattcag
     8041 gcggcgggtt catcatccac atgaccgagc gaggcacaaa gaacaactct tacattgtcc
     8101 gcttaaaaga tggcggcaaa gccatttggt ggggcacgat caatgccgaa ggaaaattca
     8161 aaggacaggg ctcggcaccg ctcgaaaaga ccagcaatac ctttgtcttg aaattggtgg
     8221 tcaaactcga ttccatgacc atatatgtca acgataaaga gatcgtcaag aatgtgactc
     8281 tgtcctcgca ggagggatgg atcgggctgt tggcctatgg cggccccgtc atttttgaag
     8341 atgtgaaact ggatgtgacg caatgaagaa acttccagcc tggctgctca ttcccctgct
     8401 tgctttttca ctcagccgcc tgcttatctt tggagcaggc atccttgccg ataccatgct
     8461 gcccactgaa gaaggtcatt ggatcgccga tgagaacagt cccttccttt ccatgtggac
     8521 aaaatgggac agtcaatatt atgtggatat cgccaccgac ggttactggt ttcgtcccaa
     8581 gcagcaaagc aatgtagcct tctttccgct gtaccccatg ttgatgcgtg tcacttcgaa
     8641 actattcggc ggcaacctga ttctgactgg ggttctgctc agcaacctgg ctttccttgg
     8701 cgccttgata tttttctacc tgttgactga acgcgaattc gattctgact cagcccggcg
     8761 tgcggtgttc tacctggcat ttttccccac ctcgtttttt ttctcctcac tgtataccga
     8821 gagtcttttt gtgcttcttt ctgtggcaac catgtattac gcccgaacac aacgctgggt
     8881 gccagccacc atcctcggca tgctgaccgc cgccacgcgc aaccttggca ttctcatgtg
     8941 ggctttggtt ctgtgggagt ggctgcgggt gcagggctgg tctttcaccg ccatgcacaa
     9001 aaagcaaacc tggctcaacc tgtggaatgg atttaaacac aactggtttc aagtcatcat
     9061 tatttcacta attccattgg gaatgcttgc ctacatttac ttccttcagg tgaacttcca
     9121 acggccgctg gcttttatcg aaacgcaggc cgcgtggggg cgtgaaaata tcggcccgat
     9181 cgccgtgctt cagaaaaata tcactgcctt gatggaaggc gaagtgaaca agggttggct
     9241 gacccgtttc tggaatgtca ccaccatgct gttcttcctt gccctggtcc ctttcatttg
     9301 gttcagattg ggggaaggtt atgccatttt tgttttgatc atgctgctgg tcccttcagc
     9361 cagtgctgtg ggcagcatca tccgctatgt gctgacacaa ttccccgctt tcatgttgat
     9421 ggggtggtgg gggcgccgcg aagccgtaga ccgtactttc ggcatgtcgt ttgccgtgtt
     9481 gctcggtgtt tttgtttcta ttttcgtcaa ttggatcttt gttgcgtaaa aggaggttcg
     9541 aatgccttat gaaaattatg cccaatggaa aatcgttccc atttccgagg agccgaaact
     9601 gtccattgtc atcccagcct acaacgagcg cgagcggatc gttgcaacga taggcgcgat
     9661 cgcttcacat gtctcagatc tcggctttcc gtgggagatg ttcatcgcag atgacggctc
     9721 aaaggatgat accgtggcca ttgtggaaga acttggcctg gtgaatttga ccgtgctcaa
     9781 agccccgaaa aatggcggca agggcagtgc ggttcaacgg gggatgctgg ctgcccgcgg
     9841 aaaatatgtc ctttttgccg atgccgataa ttccaccccc attgaagaag tttcaaaact
     9901 gctcaacaaa cttgagcatg acggcttcga tgtggctgtt ggctcacggg ccgcggaagg
     9961 tgccgaagaa ggcaagaaat cgctcttcag aaagatcctc agcgggggat tgcgctggat
    10021 cgtgaaaaac attttccgta tcggtgtgcg ggatacgcag tgcggattca aaatgtacac
    10081 ccatgaagcc gcccaaaagc tccatgccaa gcagaccatc atgggctttt cctttgacct
    10141 tgaaatttta tatctggcat ccaagtacgg atacaaaatt gccgaggtcc ctgtttcatg
    10201 gatcgatgcc ccaggctcca aagtggacac acgcaaggaa gtacagcgtt tcctgcgtga
    10261 cctgttgaag atcaaatcca atgacctgcg cggggtgtac caatgacgac gacaaatcaa
    10321 atgcgaatcg ccattgtcac cacccatcca ccttcaaagg gctctttgaa cgaatatgct
    10381 tttcatttcg tccgtttttt gaagacgaag gtggagtatg tgcaggagat cattttgttg
    10441 gtggacgaac tgcctgagag tgaatcttac tcactcggac agaagcagga cgaggttccg
    10501 attcgatgcg tgccgtgttg gaagttcggc gcgttggata acatcctccg tatccgcaag
    10561 gcggtaaaag aagttgagcc agatatcgtt ttgttcaata ttcaatttgc atcttttgcc
    10621 gataaaaaaa ttcccgccac gctgggcttg ctgactccgt ggtttttgaa ggtgtctggt
    10681 tttaaggtgg cggttctact ccataacatt atggagacgg tgaatttgaa atcggcgggg
    10741 tttggcagca acccgctgat cgaggcggtg attcggttga tgggaaatat cgtcacccgc
    10801 tttttgctgg ctgccgatct ggtggcggtg accattccca agtacgttga gattcttgaa
    10861 agcaaatacg atgcaacgaa tgtgttgctg gcgccgcatg gatcgtttga agaacccacc
    10921 gatacagagc cgaagcccct gccaggcggt acgcccacca ttatgacctt tggaaaattt
    10981 ggcacctaca aaaccgtgga cctgctgatc gagtcgcatg ccatgttgca aacacaacac
    11041 cctgatctga ggttggtcat tgcaggcacc gacagcccga atgcagcggg atatttgaag
    11101 accatgcagg aaaaatatgc ccatgtgaaa aatgtggagt acacgggtta tgtggctgaa
    11161 gaggatgttc cacgtatttt tggtgaagca tgggtcgtgg cttttcctta cacatccaca
    11221 accgggagtt cgggtgtgtt gcatcaggcg ggtggttttg gaaaggcggt cgtgcttcca
    11281 aaattgggcg accttgccga attggtgctt gaagaaggat acgacggcga attttttgaa
    11341 ccgacgaatt caaagagcct tgccgatgca attgcacgcg tgattgataa ccccgagcgt
    11401 caaatggaga tcgggctgcg aaactataca gctgccaacg gcctgccgat cactgaagtg
    11461 gttgattggt atctcatcca tttcgaagaa ttattgaaaa ggaaataagg agtttttatg
    11521 gaaatgatca aggtggtgaa tggaccgatt tcggtgttgc agctcaaagg tcgttttgat
    11581 gcgcatgaag tggagcctgt caacgcctgg ctgcaggaac aggtgaatca gggcagggtg
    11641 aaggtggttg tcaatctgga gggagtcaat tttatagatt ccactgcgct ttctgtgctg
    11701 gtgcgcggaa tgaagaactg ccgtgaaaag tcgggtaatt tgaatctgtg ttcgctgcag
    11761 cagcctgtgc gcgtgatctt tgaattaacc cgcctcgaca aggcgtttgg aatttacgca
    11821 tctgaaactg aagcaggcaa ggcattttag gaaaccatgc tggctgaaca cttggaaaac
    11881 caccgcaaaa agttttattc gcttgcatct gtgtggatgc gggtgggtgc aacttgcgtg
    11941 gaattgatgg aagctggtca ggtgctggcg acttttccgc aggagtgcag tgattcttcg
    12001 cccgccctct tcgttcatgc gcggacctcg aacctggctc tgcgaattca cggaatcgtg
    12061 gacgagaatt gcaggccgat cgctgaatcg atgcttgata tcctttccaa tctgctcacg
    12121 gctgacctgg aactggaaaa tctcactgct gccctggtgg agactcaaga caggctggtg
    12181 gcgatctatg aattgtcgca ggcttcccgc cgcaccttgg aaattcccgc cctgctcgat
    12241 ctactggtgc aagaatcaac tcacctgatg aatgtggatg gcggttttgc ggtgttgatg
    12301 gagaagggca agaagtctgt catccatcag gtttccgatg cccccctgca ccatgcgcat
    12361 ttggaagcgg cggcgacctt gttccgccgt gacccaacac gccatacttt taaagaatct
    12421 gaaactctgc ccccgggttt gagaaatgta atgatggttt cgttgcctgt gcgtgatgag
    12481 gtctttgccg catttggggt tttcaataaa acgggtgatt ttacttctcc agatattaag
    12541 ttggcgaaag ccattgcagg ctatgtgggt gcgcggcttg aaagtgcctt cctgcataag
    12601 gaggcccttg aacgcgcccg catggaaacg gaaatggata ttgcccgcca ggtgcaaatc
    12661 gccatccttc cacaatccct gccaaaggtg acaggactcg atatttatgc cacctccaca
    12721 cctgcactcg aggtaggcgg cgatttcttc gatgtcattg atcgctcgga agattcgctg
    12781 gtctttgccg tgggagatgt gacaggcaaa ggtatgcctg ctgcgctgtt gatgagtatg
    12841 acgcatacgg tcatcaaatc tgcatcgcgc aaaatgccgt tctcacatcc gcatcaggta
    12901 ttgaacaggt tgaaccacga tctgttcagc gatttttcca atgtgggcat gttctccacc
    12961 gtttttctgg gcctgttgga tcgcaagaat tgcaccgtga cgtttagcaa tgcggggcat
    13021 agtccgattt tctacattcc cgcggggtgt gaagcccatt tactggaagc ccaggatatt
    13081 cccatcgggg tgctggatat ttacgattac acatcccaaa ccatcaaatt tgcgaagggc
    13141 gatatttttg tcattgcttc agatggtttc cccgaatcgc gtaattcttc agatgaaatg
    13201 tatggctatg accgcatgaa ggcctgcctg gagcgttcgc ggaatgcccc tgctgaagaa
    13261 atggtttccg cgcttttggc agatgttaac gccttttcgg ggtcgcatct tcaggatgat
    13321 gaccgaacca ttctagtgat caaggtgaat gaattaagaa tgcagtctga attgatcaat
    13381 atccgttcca cttacaagga aattcgcctg ccttcggaac gattgcgagg cttgctggct
    13441 tacaaccagg tgacggaaga gatcatcaat ggctgtgaac tcgccctgca tgaactcctc
    13501 accaatctgg tcgaccatgc ctacgaaggc aatgaaaatg gaaatatcac cgtcaacatc
    13561 acttgtgata ccgatttcgt cacgatcgag acccgtgatg agggcaagcc tgccaatgta
    13621 gacatggcag ggattgccat gccagatcca actgatttgg ccgaaggcgg gtatggcatg
    13681 gctatcatcc aaatgctgat ggatgacgtc aaatataaat ttgaagattc aaagaatgtt
    13741 tggcggctga tcaaacgtat tcaatgagca ataaatagaa agaggaactt cccatgacac
    13801 ttgaaattag catcagtgaa caggttatca agatcgccac gatcgctctg aatggcaggt
    13861 tggatgcctt ggaggcaccc gccctgcgga aaagggtgga tgattacctg gcaaacgaat
    13921 tctcacactt tgtgttcgac ctttcggatg tagccgtgct ggactcggcg gggctggctg
    13981 ttctggtcag cgcgttgaag cgcgcccgct tgaagaatgg cgatgtgcgt ctggtgtggc
    14041 caaaggccga ggtggcatca cgcattttga agctgaccaa atttgaccag gtgttcgtat
    14101 ccattgaacg ttctgagatc gtaccgcgag ggttttagca tggcacgtat cctgatcgtt
    14161 gatgatgaac ccaacaacca gcgcatccta aactacacct tgaacaaagc tggctacgaa
    14221 accattaccg ccgccaatgg agaaaccgcc ctggagttac tggaccagta cgatgcagac
    14281 ctcgccattg tagatgtggc catgcctgtc atggatggtc ttaccctgct caagcatatt
    14341 cgctctgcag ataaatttga gcatctgcct gtcattatcc tcacaggcag cggcgatgat
    14401 gaggaacgtc tgcgggcgga gcgtgaaggc attcagggtt ttctgaccaa gccttccagc
    14461 tcaaagatgg tgcttgaaac ggtgcaaaat attttgaaac gatgatacag aaaataaaag
    14521 aacttctacg atcaaagatc gcatcggcca gtgctttgct catggtcagc atgacggtgg
    14581 tcaatgcggg gaactacgta tacaacctca tcatggggcg ctggttgggg ccgtcgcttt
    14641 ttgccgacct ttcgttgatc gtcaccctgt tgctcattgt cacattcatc actgctccga
    14701 tacagatgac gaccgcccgt tacacggcca tccatgctgc ggatgcggat gttaagaccc
    14761 tcgcttcgat tcgacggttc atctggcgca tcgccgccgc gcttggtctt acgctgacgg
    14821 ttttattcgc cacttttgcg ccgttgttgc aaagtttttt tcacacgcaa tcatttttac
    14881 ccttcatcat ttttggggcg gcgctgccat ttagtttgct gcagggtgtg gagcgtggaa
    14941 ttcttcaggg gcggacccat ttcaaggttt tggcgatcag ctatcaggtt gagatgtgga
    15001 cccgcctgtt tgtcagcatc atattggtgg cgctgggatt atccgtcaat ggggcggtca
    15061 ttggcatcag tctttcgttc ttcgccacat ggctcacagc gcggctggct gtgcgagatc
    15121 ttttcacaaa ggaagatatc cagaccgaat tacgcaaaga gttgttcgcc tttgccgtgc
    15181 ctgtgctggt ttcgcagttg ggacagatct tgatcaataa tagtgatgtg atcctggtga
    15241 agcgcttcta tgaatcagac caggctgggc agtatgccgc actggctttg atcggacgta
    15301 ttgtgttttt tgcaacctgg tctgtggtga ccaccatgtt cccgatcgtg gcgcaaaagc
    15361 aaaaacaggg ggagcctcac agtcatttgc tttgggtttc gcttggcatt gtgttgggcg
    15421 tgtcgttgcc cattgtttta ctgactttgt tcgtgcccga gtggatcgtg aagatcttgt
    15481 tcggcagtca ataccttggc attgcaccgt tgctttggct gtatgcgctg gccaccacct
    15541 tgtatgcgct ggcgaacgtg gtcatcaatt acaggttatc gcttggcatg ggaagagaaa
    15601 cgcgctttgc gcttgcggcg ggcgtggcac aggtgcttgg gatcatcttc tttcatcaga
    15661 cacttgcgca ggttgtttat gtgcaggtgg tggtgatggc ggttttgttt gccagtttgt
    15721 tgtggtggaa ttttagcggc cgcgccagac aactgccttt gatacaggtg gctaaaagca
    15781 atgcttgatc ttttcttttc cgtggcgatg gatgcactga tcgcctttga tgaatctggc
    15841 agggtcatcc gcgcgaatca atttgctgcg gagatgttcg gctattctat ggatgaattt
    15901 cgaacaatga ccgtggaaaa cctcctgccg aagaagaacg caaaaaatcg taaagatctg
    15961 gttttgttgc tgttgaatac taacgttgaa aatcgaaaaa tcgtaaaatc tcatcgggta
    16021 tgggttctgc ataaaagtgg ttatgaattc cttgtaatgg cctccattgg gaaggggagt
    16081 ctcaatgatc aacctgtttc gcttatatct ttgagagact ccttgattga cgggcaggaa
    16141 gaaaacctga ccgatctgcc catcacggat gcacgggaaa accccaatgc tgagattcgc
    16201 attggtagag atggaaggat cctttttgcg aacaaaagcg gtaaacggct tttaaggcgt
    16261 agtggaatgg tggttacggg acaggtcccc tcagagtggg tgggcttttt tgagcaggct
    16321 ctcaaaagaa aaacacatat tcaaagggtc attcaacatg gccgcaggtt atattcctgt
    16381 cactttaatc ccacacccga aatggggtat gtcaccctgt acgctttgga tatcacagaa
    16441 tatgaggccg aaaagaatag acttgcgctt tccgatgaaa ttctgcgttc gatcggaaac
    16501 ctggtcctgg tggctaacag caatgccgag gtggtgtata tcagcccatc ggtgaaggag
    16561 atccttgggt atgaacccga agaactgatc ggaaatggct ggtgggaatt ggagcggatc
    16621 tccggggggg atgtgctggc ggaacgggat tacattagca aggccgctgc cgggctgcgt
    16681 caggtggatg acaagccgta tgaacatcgg gtacgccata aaaatggtga ctggcgttgg
    16741 ttgatgctgg ccgaccgaaa aggtccgcgt gaccttttga tcgggatcgg cacagacgta
    16801 actgaaagta agaagacagg cggagaactg caaaatcaac gtgactttgc ccaaaccctt
    16861 accactcaaa tggggcaggg gttgaccgtg atcgatgaag aaggccactt cacgtttgca
    16921 aacccatact ctgcgaaaat attgggatac gaaacggagc aactggccgg gttgaccgcc
    16981 tttgatgtca cttttcctga agaccatgag ttggttaaaa cggtgttgtc gaaacgcaat
    17041 gtaggtgagg tgaccacgct tgaaatacga ttgcgcggta gaaatggtga tgaggtgtat
    17101 gcccttgtca ccagcgtgcc tcatgttgta gatgggcagt tcgctgggac gatcaatgtg
    17161 atcaccgatc tgacggagcg gcggcagatg gaaaacctgc tccgtgatta tgccaccgcc
    17221 atcgaaaaaa gtaatattga tctggcagat gcccgcgacc gtgctttgga agcttcgtat
    17281 ctcaaaagtt ccttcctggc caccatgagc cacgagattc gaactccgat gaatgccatt
    17341 atgggcatga ccgaaatgct cctcagcaca gatttgaatg acgagcaacg ggagtttgcg
    17401 cagaccatcg attcctccac gcaaggcctg cttgccattc tgaacgatat ccttgatttt
    17461 tccaagatcg aagcgggcaa actttccatc attccgtctg tttttaaccc tgtagagatg
    17521 acccgtgatc tgatcaaact attccagccg cgggcgcagg agaagaacat cactctctcc
    17581 atgatggcga ccgcatccat tcctgagacc ctcgttggtg atggcggcag gattcgtcag
    17641 atcctcggaa attttgtcag caatgccatc aaatttaccg agaagaatgg ctgcgttttc
    17701 atcaatattt cgggaacaag catcaaccag aacacgttga tgaccacctt taccgtgcag
    17761 gataatggaa ttggcgtctc tgatatgatg cgaagtaaac tttttgagcc tttcacccag
    17821 gctgatggct cgaacacccg caaacacggt ggaacgggcc ttggcctggc catttccagg
    17881 cgtttggtgg atttgatgca tggtgagatt ggatttgaat cgtttgaagg tacagggtct
    17941 acgttttggt ttagcctgcc cttgtatacc actgccccaa aggatcagga agaaacagag
    18001 ttttcttccc gtgggatgca aaagaaatac ctgaattatt cgagcagaaa accgatcttg
    18061 atcgttgaag acaacctcgt taaccgtgac ctgttcactc tccagttaca ggaatttgac
    18121 ctcaccataa aacaggctgg gaacggcagg gaggcggttg agttgctcca aatacaccct
    18181 gatgactttt cgctggttct gatggatgta cacatgcccg aaatggacgg tttcaccgcc
    18241 acccgtctca tccgcaagag cgaagcagga acgggaaggc atattcccat cgttgcaatt
    18301 accgctgatg ccatggtgag cagtaaaaag ctgtgcctcg aagcaggcat ggatgatttc
    18361 ctcaccaaac ctgttacgct tgccaacctg gataaattat ttagga
//
LOCUS       DAOQLC010000089        18367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1060_length_18367_cov_24.440640, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000089 DAOQLC010000000
VERSION     DAOQLC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1060_length_18367_cov_24.440640"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PL000_14785"
     CDS             complement(<1..503)
                     /locus_tag="PL000_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90202.1"
                     /translation="MRKTCIDFMKTIKNILLVLSTGYIFVFFSEHLFWARIRPEDTLK
                     DWVGAWIAYSLMAYVFLILITHFKVRNIWPLFLAGAAFGWLAEGLVVQTTYEMLPLSI
                     SFTGLAWHALITIWIGWYALQRSFHSPASFSTWKLVAAIGACSALWAIAWWLEPDGGV
                     ASVAEYA"
     gene            complement(613..1365)
                     /locus_tag="PL000_14790"
     CDS             complement(613..1365)
                     /locus_tag="PL000_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90203.1"
                     /translation="MKQKTLIPGLVLLLSIIACNLPSGNPPATQTPIVQIITATPADT
                     PVPTFTLIPTETALPTFTPTPTIPIAWPLDKGVNCRYGPSTDWVATSSLLVGQTATIQ
                     GKNGDASWWYVTTPEDPGNPCWVSASVTVTAGNLANLPVIKSPTASVTGVDVKVDPKE
                     INLPGCLGPVQPIKINGSITVNGPTKVDWYFETQQGGSMGTYTLEFDKADTKKVDANF
                     NPNPAAGDFWVKLITITPNGKVAETKYKINCP"
     gene            1502..2242
                     /locus_tag="PL000_14795"
     CDS             1502..2242
                     /locus_tag="PL000_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNC90204.1"
                     /translation="MDPVLHDQLINEVLHHTQTDPHKRASGLSDFILGAQDGLVNVLG
                     VVLGIAAATNDARIVLVAGLATTFAESISMGAVAYTTTLADADLYQSEREREYRHILE
                     APNLEIKEIRDIYASKGFKGELLDHIVNTITANQDVWVAVMMAEEHNLAPVDRKTAVR
                     AAIVVGVSAILGSLVPLFPFMFWSVSVSMWLSILVTALVLFGIGAYKASVTVGKPMKS
                     GLEMAVIGTVSALAGYLVGVILKVPPVP"
     gene            complement(2244..3137)
                     /locus_tag="PL000_14800"
     CDS             complement(2244..3137)
                     /locus_tag="PL000_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90205.1"
                     /translation="MSDYAVPQHIFAETVATHMQRNNLIPGVLILKGDQSIGVIPRHK
                     MFERLGKRYGVELFLRKPVGELETELGITPFILKHHLPINVAVKLALSRSEGNIYDPI
                     VVEFEDGSRSLLDMYVLLLTQSQLSTNLSGIISSLNNIETILANGMAGASTLNLILES
                     MGSVVPYHHARILLQYQQSISELAGLKDTVQTSHEPLERNDIYRSILSINQPVSLEDV
                     RIVPTWKGSEFPSITRSWMGIPLSIQNGIVGLVALSRFTYSPFTNNEKELGLVFTRYI
                     STLLSDLTNRTKRARSYEKLF"
     gene            complement(3302..3622)
                     /locus_tag="PL000_14805"
     CDS             complement(3302..3622)
                     /locus_tag="PL000_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90206.1"
                     /translation="MTLVLIYTLIAGVIAAVTAPIPGTSLLLTGLELYMLVHLAKTNG
                     YKLGMKEIGYTAAAIYGLSTLLQDVALELLTFVPVIGWGAEVVVAVIFVFFLGMLANL
                     YFKK"
     gene            complement(3619..4116)
                     /locus_tag="PL000_14810"
     CDS             complement(3619..4116)
                     /locus_tag="PL000_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90207.1"
                     /translation="MATIIFNGKSYNSLEEMPEKERLAYEQMMGIFADKNGNGIPDFM
                     EGDLVQKVMAVNSTHVNINANGQTYQNLDDLPPELRQRVDKAFQVMTNFGIIPGVPEE
                     LKMNNQQANREPTAQSKPFVSREYTPAIQEESGTSIFPWIIGGIMLVLCLAMAAFGVL
                     YYIAR"
     gene            complement(4127..4438)
                     /locus_tag="PL000_14815"
     CDS             complement(4127..4438)
                     /locus_tag="PL000_14815"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC90208.1"
                     /translation="MQQWLYQLKPSRLEMVTDGPTPEEAEVISRHFAHLQELSRNGVM
                     IMVGRTQNNDESTFGICIFEAEDESAARTIMESDPAVGAGVMRAVLYPYKIALMKGQP
                     E"
     gene            complement(4455..4928)
                     /locus_tag="PL000_14820"
     CDS             complement(4455..4928)
                     /locus_tag="PL000_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90209.1"
                     /translation="MRKRAMVMLGFFAGFLLFFLPGILLVNTPGFATLGGILLGLAVL
                     MLVINIGYSIMGMIQPLWVRQVLQSGTEAKAIIIKNNAITGIGGYKGSDIWLDLPVRV
                     QPANGSAFEAQMKCRLTQTLMLREGSEVTVRYDASNKTKVALVGDARTDMMTKYM"
     gene            complement(4960..5202)
                     /locus_tag="PL000_14825"
     CDS             complement(4960..5202)
                     /locus_tag="PL000_14825"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HNC90210.1"
                     /translation="MTDEKVCPKCKGAMTQGRIMKYNEYTTHNRYLYVFAPDGEPGPD
                     LSKMFSASPMSAARKGLVAFCCDQCGFVEFYGQVIP"
     gene            complement(5273..>7198)
                     /locus_tag="PL000_14830"
     CDS             complement(5273..>7198)
                     /locus_tag="PL000_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90211.1"
                     /translation="EKGARLGLVGPNGVGKTTLLRIIVGEDDASTGNVSRNRGVRIGY
                     LSQEADFKMDGTLWDACHSVFEELIQKQEELHKLESQMATNPDVIERYGKLQEDFERR
                     GGYTYETRIKQVLTGLGFDAEDYSLSLDHLSGGQRTRAFLSRLLLSDPDVLLLDEPTN
                     HLDIRAVEWLEGYLSQWEGAAVIVSHDRYFLDKASNVILEMLPGAYEIYNGNYTAYLK
                     QREERITRRQEVFESEKEKLLKEVEYIKRNISGQNTQQAKGKLKRLSRIVQAVEQIGF
                     EAALNQKWSETAEDVHVTTSNFGVEEAERRVRALRSPVRTLPHLHLRLGSEKRSGDLV
                     VRTKDLEVGYPGKMLFKTPDIELRRQDCAALIGPNGAGKTTFLKTILGQLEPLAGEVV
                     LGASLQIGYFAQAHEGLDPNRTVLQEIDSVMPHWLPGQIRDYLGKYLFSGDDVYKKVD
                     MLSGGERGRLALAKLALQDTNLLLLDEPTNHLDIPSQEVLEAVLEDYAGTILLITHDR
                     YLVDAVATQIWEIDVDGSHLTAFNGTYSQLRAEREKQEYLAFMAAQGAGETSSTSNVK
                     RAPSNVKTKEERRKIAQLQELENRIAELEKQLADIGSQLENPPSDAGEVVKLSKAYES
                     TQKEMDEKLAEWETLQG"
     assembly_gap    7199..7298
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7299..7798)
                     /locus_tag="PL000_14835"
     CDS             complement(<7299..7798)
                     /locus_tag="PL000_14835"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90212.1"
                     /translation="MKPPEVIITERLTLRKPRMDDAPAVFEGWARHPEVTRFLTWRPH
                     TCVEQTQELMKRSMAAWDGETNFRYLLEIRESHALAGMIELRMEPYKMSFGYTGAFAQ
                     WGKGYMTEAVCASIVWAFEQPTIFRVYATTDVENIPSRRVMEKAGMQCEGILRKDSMH
                     PNLSNVP"
     gene            complement(7824..8987)
                     /locus_tag="PL000_14840"
     CDS             complement(7824..8987)
                     /locus_tag="PL000_14840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90213.1"
                     /translation="MKIRLQLTIFVFLRTILNTAHRMVYPFLSVFARGLGVDIASMSL
                     LMTARSLIGSASPLFAPIADRRGRKFGMLAGISIFTLGMGIVAIHPSYWTLAAALILA
                     VLGKYMFDPAMQAYFGDRIPYEQRGTALAVTEMAWSMAFIIGVPAMGFLISKFSWSAP
                     FPVLAALGALVFFIIWRIIPHEDEHHEPVANSREGYSAVFKSIPALAGISIAMWASAG
                     NELVNVIFGVWLEDSFGLKIAALAGASAVIGISELSGEGLVALTTDRLGKPLALTIGL
                     VGNAVASLLLPIIGTTQIGALIGLFFFYITFEYIMVSHIPMMTELVPSARATMLSLNV
                     TGHAVGRALGAFLAAIIYQRFGFVPVALLAVIFNIAGLLALKRMQRQETDRAR"
     gene            complement(9029..9634)
                     /locus_tag="PL000_14845"
     CDS             complement(9029..9634)
                     /locus_tag="PL000_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90214.1"
                     /translation="MLKTKSPLLFFLLTIGAIALLALFGPEEQSLGSNVRIVYLHGAW
                     VLTAELAFAAAALTGLLGLLLKNDQLHAWSAALGRTGIVYWLTYLPLSLFAMQANWNG
                     LFLAEPRFRLAMVFAITGILLQAGLWLFNISWLTSLGNIIFIVVLRVIFSTAQNIMHP
                     PPSPIFNSGNYVIIGFFVGLNLLAWLAAYFLAKWFLQLKAN"
     gene            9760..10629
                     /locus_tag="PL000_14850"
     CDS             9760..10629
                     /locus_tag="PL000_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNC90215.1"
                     /translation="MEYWPLIVIVLQLVFLEGILSIDNAAVIGALVSPLPENEDVPWP
                     GKLKKLGTVLHPFLGFQRMAALRVGLLGAYLGRGAMLFLTSFLIHNSWIKLIGAVYLI
                     HLAFDNLEDMTGDSEEEGGLSPIKVRSFWATVMTVEIMDLIFSIDNVVAAVSLSDKIW
                     VVMLGVGIGILTMRYAAGIFSYAVQHEPILKQAAYILVLNIGVELILDQVWHVEIPDL
                     LRFGISVATIALAIAYAHFPFLQKFRFVLVWLAKGIGIVNEFVDWLFIPFKELFNLIA
                     SLFVRPAGTHPAE"
     gene            complement(10708..11151)
                     /locus_tag="PL000_14855"
     CDS             complement(10708..11151)
                     /locus_tag="PL000_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90216.1"
                     /translation="MPIIKTPKTREEFKAYYSLRYKVLREPWGHPKGTEKDDYEPISE
                     HFMAVDEKTGEVLGVIKLFEKAPGVGQVSHLAVAQEHQHKGIGKFLIVEIEKRAHEHG
                     FKTLGAMARVTATAYFEKYGYHIAGIPTPHLGTTHLVWMEKELSS"
     gene            11436..14351
                     /locus_tag="PL000_14860"
     CDS             11436..14351
                     /locus_tag="PL000_14860"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC90217.1"
                     /translation="MAIEKKEDMLQENAWHALNAEQVLRDLEVHEEGLTSAEAEKRLA
                     HYGPNQLREAPRPGFLALLWGQLNNFVVILLIVASVISALLGDYIEAAAIMAIVVLNA
                     VLGIVQEQRAEQALAALKKLAAPDARVLRDGAHTSVPSYNLVPGDIVFLEAGNFIPAD
                     LRLLEAVNLRVEEASLTGESLPVQKNAATVLDKNVPLGDRKNTAFMGTVVNYGRGRGV
                     VTSTGMRTQLGLIATMLQNVETEETPLQRRLDQLGRSLSIGSLILVAVVFIVALFNYT
                     EISGLFSSPLDYLKEFASEITEVFIIAISLAIAAVPEGLPAVVTISLALGMREMVQRH
                     ALIRKLSSVETLGSATTICSDKTGTLTQNEMTVTRVWADNQFVHITGTGYAPNGEFQM
                     DGKTVDVTKYPAILSALWLGILNNDATIETTGETESQRTYRIVGDPTEGALLVAAAKA
                     GAIHVDIDEAYPRENEVPFDSERKRMITIHDVRSPKPTDPSPFYDEKHKDWDVIAVKG
                     APDIVLELCTKYLGMDDKPREMTKEAKERVLAANDAMTKDALRVLGFAYRVEKDVPDN
                     PENIKADELEKDLVFVGLLGMIDPPRTEVKPALEKARHAGIRTVMITGDFPNTAKAIA
                     ESISLLRPGKNVMTGAQLDAIDDNELKRIIEDTDVFARVSPEHKMRIVDALQANDEIV
                     AMTGDGVNDAPAIKRADIGVSMGITGTDVAKETADMVLTDDNYASIVSAVEQGRIIYS
                     NIRKFVFFLLSSNVAEIMIIFLATLAGLPAPLTAIQLLWLNLITDGAPALALAMEKGD
                     PDIMDQKPRAKNEPIVNRSMGIGIIVQTIAQTSAVLGAFVMGLAWHLEAGAVLPSGMN
                     VLSFVLSHDWRGVDVQTAETMAFVTLSLAELFRAYTVRSERASIFQIGFFSNKYMQYA
                     VGISIALLLLVCAVPFLQPIFNTHFLSGREWGLVLGLALMPAVAEEITKFFLRRSRA"
     gene            14535..15677
                     /locus_tag="PL000_14865"
     CDS             14535..15677
                     /locus_tag="PL000_14865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HNC90218.1"
                     /translation="MFRFQGEPFTPEQKTKFHFFASILALALIAVVVAVLFQPLILPR
                     MDFYNELWGPSYLLVHRQSPYDTASLNPVLPAVWFPMAIGFFFPLGWLSEDAALQAWF
                     FFNIVEICIVVFLVQGKDRSLYNTAALALLCFFFPFVLNHFNLGQFSITILLCLLLSA
                     HWIGKYKNWPSAFFLALALAKPQLGILAVIGLALHQFRQRGFAGMFLFGAQTLLMALL
                     MSLPLFIAHPAWIPDWIASMAKNYTWQHPSLFSVFQQMLGRWGLVPWAAIAAGVLAFT
                     FLLWKKIPPFAAMLWSLALTTLVTPYVWSWDFVLLLPLWMFSFTKANWKWKILLLVSY
                     LIAWYGMALVQMQAGSQNQSFWWVPLWFIGTLVPAWRMGEVKYFDS"
     gene            15708..16991
                     /locus_tag="PL000_14870"
     CDS             15708..16991
                     /locus_tag="PL000_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC90219.1"
                     /translation="MPRFIRVTFFLALLSLTLACGLFQSAPSVRDPLWVYANPNFSPD
                     YASIQSSESGLVTYQSGASNELPISLITLNPASGEEIFTPTPNDELVIFEGVAAGRAI
                     FVGRVSDQVNEDGDVLYPIIALDPSTGLEVWRYDGSVFESGIPRTEKYLFVSKRYSQI
                     DVVDLMNGEVVASFTKEYDAAELFDLNPGWIRYMYTDTAIYSLSPAGVLRRYDLPTLT
                     LQSTLNLDIPHYLDAFFIEGDRLYVYSAEVLDADLDLLAFDLASGTYLWELRGMRGST
                     VGLEFYNGFGYVDTVNGPSKIDLSNGQVLWSVSVQLAPFFIGDDSNGVFLATQDGLLT
                     AYDSTSGKELWTYRTGQPNSINLSAVNGVAFITTAEDLAAFESFFVPNQLDAVDIATG
                     KALWRYKNPYVTLPIGTDNAFIVGYYGGITALPAK"
     gene            complement(17047..17472)
                     /locus_tag="PL000_14875"
     CDS             complement(17047..17472)
                     /locus_tag="PL000_14875"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNC90220.1"
                     /translation="MNCPKCNTELAKKYYKGMIEVDSCPNCRGMWLDFNELDKLEDVA
                     FDDDSRKGSLIHHQAKTEFPCPHCGAAMDEFQYRLHDLKLDYCAENDHGFWLDAGEDE
                     RVIAAMNQRAQDIQRKNSAESDWRQTLKDMHAFLRKWKP"
     gene            complement(17537..>18367)
                     /locus_tag="PL000_14880"
     CDS             complement(17537..>18367)
                     /locus_tag="PL000_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNC90221.1"
                     /translation="IRLDVLLVERGLAESRAKAQALIMAGQVRVNDQVTLKPATAIDT
                     KSALTVDTGPRFVSRGGEKLDAALERFGIDVKDFICADVGASTGGFTDCLLQRGAAKV
                     YAIDVGKGILHWKLRNYARVVVMEETNARHVESLPEAVSLVTVDASFISLKILLPVVK
                     KWFSFFPLSSFNDDEREKKKEGIVALIKPQFEAGKKDVARGDGVIRDPEIHRQVLLDV
                     LTFAKQEGFNLRGLIKSPLLGPKGNAEFLVWLDFQENGRSVEDLVDEAMAESTSNLTE
                     "
BASE COUNT         4180 a         4633 c         4738 g         4716 t
ORIGIN      
        1 ctggcatatt cagccaccga tgcgaccccg ccatcaggtt ccagccacca ggcgatggcc
       61 caaagcgctg aacatgcccc gatggcagca accagtttcc aggtcgagaa agaagcgggg
      121 gagtggaacg acctttgaag agcataccat ccgatccaaa tcgtgatgag tgcgtgccat
      181 gccaatccag taaatgaaat tgagagtggc agcatttcat aggttgtttg cactacaagg
      241 ccttctgcaa gccagccaaa agccgcgcct gcaaggaaca gcggccagat atttctcacc
      301 ttgaaatgtg tgatgagaat caaaaagaca taagccatga gcgaataggc gatccatgcg
      361 ccgacccaat ctttgagcgt atcttcaggg cggatgcgtg cccaaaaaag atgctcggaa
      421 aagaatacaa agatgtagcc ggtggaaagc acaagcagga tgtttttgat ggtcttcatg
      481 aaatcaatac aggttttgcg catgaaaagt tgccgataca aaaaagcgac ccgtttgaaa
      541 cggaatcgct ttttgttgtg ttccttgttt tcttttgaat gctaaaaaag gagctttggt
      601 atcaagacca ttttatgggc aattgatctt gtatttggtt tcagctactt ttccattggg
      661 tgtgatggtg atgagtttta cccagaaatc tccagcagcg gggttgggat tgaagttggc
      721 atccactttt ttggtgtcgg ctttgtcgaa ttcaagggtg taggttccca tggaaccgcc
      781 ttgctgggtt tcgaaatacc aatccacttt ggtcggtccg ttgaccgtga tgctgccgtt
      841 gatcttgatg ggttgcacag ggccaagaca gccaggcagg ttgatttcct tcgggtcaac
      901 tttcacatcc accccggtga cggatgctgt gggtgattta atgacaggca ggttggcaag
      961 gttgcctgcc gtaaccgtta cgctggcaga tacccaacaa gggttgccag ggtcttccgg
     1021 ggtggtgacg taccaccagc ttgcatcacc gttcttgcct tgaatggtgg cggtttgccc
     1081 gaccagcagg gaactggtgg ccacccaatc ggtgcttggc ccgtaacggc agttgacgcc
     1141 tttatcaagc ggccaggcaa tgggaatggt tggagtggga gtgaaggttg gcaatgccgt
     1201 ttcggttggg ataagggtaa aagttggaac gggagtatct gcgggtgtgg ctgtaatgat
     1261 ctgtacgatg ggagtttgtg tggcgggcgg atttcctgac gggagattgc aggcaatgat
     1321 ggaaagtaat aaaacaagcc cggggattaa agttttctgc ttcataaaat aacttctcct
     1381 tttcatggaa aagaatataa acaacatggc agaaagacac aatgccccat tggttttgtt
     1441 gatgcaagac tggtgacatt tgtcaattct cagggctgtg ttgtctgagt atcattgttg
     1501 tatggaccca gttttgcacg atcaactcat aaatgaagtt ttacatcaca cccaaactga
     1561 cccgcacaag cgggcttcgg ggctttctga ttttatttta ggcgcacaag atggattggt
     1621 taatgtgctt ggcgtggtgc ttggcattgc cgccgctaca aatgatgcac gtatcgttct
     1681 ggtggcgggg ctggccacca ctttcgctga atccatttca atgggggcag tggcctacac
     1741 caccacactg gcagatgccg acctgtatca aagcgaacgt gaacgggaat atcgtcatat
     1801 tttggaagcc cccaatcttg aaattaagga aatccgcgat atttatgcca gcaagggctt
     1861 caagggagaa ctgctggatc acatcgtaaa taccattacc gcaaatcagg atgtgtgggt
     1921 ggccgtgatg atggccgaag aacataacct tgccccagtg gaccgaaaaa ccgcagtgcg
     1981 cgcggcgatt gtcgttggcg tttccgcgat ccttggttca cttgtgccgt tattcccctt
     2041 tatgttttgg tcggtatcag tgagcatgtg gctgtccatt ttggtgacag cccttgtgtt
     2101 attcggtatt ggcgcctata aggcatctgt cactgtgggc aaacccatga aaagcggact
     2161 tgaaatggcg gtcatcggaa ccgtcagcgc gttggctggt tatttagtgg gtgtcatcct
     2221 caaggttccg cctgtgccat aggctagaag agtttttcat aagagcgagc cctttttgtt
     2281 ctgttggtca aatctgacag aagagtgctg atgtagcggg tgaacaccag tccgagttct
     2341 ttttcgttgt tggtgaaagg ggagtaggtg aatcttgaaa gcgccacaag cccaacgatc
     2401 ccattttgaa tcgagagcgg aattcccatc catgaacgtg tgatagatgg gaattctgag
     2461 cctttccatg taggtacgat acgcacatcc tccagactca caggttgatt gatggaaagg
     2521 atcgagcggt agatgtcatt tcgctccaga ggttcatggg atgtttgaac agtatctttc
     2581 agaccggcta attcgctgat actctgttga tattgcaaga gtatccgtgc gtgatgatat
     2641 ggcaccaccg aacccatgct ttcgagaatc aggtttagag tgcttgcacc tgccattcca
     2701 ttggcaagga tggtctcaat attgttgagt gaactgatga tgccgcttag atttgtcgaa
     2761 agttgagact gcgtaagcag taacacatac atgtccagca aagaccggct tccatcctcg
     2821 aactcaacca cgatggggtc ataaatattc ccctctgaac ggcttaaagc aagtttgaca
     2881 gccacgttga ttggcaggtg atgtttgaga atgaatgggg tgatgcccag ttctgtctcc
     2941 agttcaccaa cgggttttct gagaaacaat tccactccat atcttttgcc cagtcgttcg
     3001 aacattttat ggcgcggaat gactccaatg gattgatcgc cttttagaat aagcacccct
     3061 gggatcaggt tgttacgttg catgtgcgtt gccacggttt ctgcgaatat atgctgcgga
     3121 acggcataat cagacatggg caaatcccca atgcagacag gaatggggat ttgcccggta
     3181 gggaatactt tgaaataatg gggaatggtt tggagttctc tcatggatgg tgagagaatt
     3241 gtatggcgca gggtttaatt gggtttgaaa agcaggttaa cggtcggtaa agaaacgatt
     3301 cctacttttt gaaatacaaa tttgcaagca tccccaaaaa gaaaacgaag atcacagcta
     3361 caaccacttc cgcgccccag ccaatgaccg ggacgaaggt cagcaattcc agggccacat
     3421 cttgtaacag ggtggagagc ccataaatag ctgccgccgt ataaccgatt tccttcatgc
     3481 ccagtttgta tccattcgtt ttggcgagat ggacaagcat gtaaagttcc agcccggtca
     3541 acagcagaga tgtgcccggg atcggcgcag tgacagcggc gatgacgcca gcgatcaggg
     3601 tgtaaataag aacaagggtc atcttgcaat gtaataaagc acgccaaaag ccgccattgc
     3661 cagacacaac acaagcataa tgccgccaat gatccacgga aaaatactcg tcccgctttc
     3721 ctcctgaatg gcgggggtgt attcacgcga aacaaaaggc ttggactgtg ccgtcggttc
     3781 gcggtttgcc tgctgattat tcattttcaa ttcttcgggt acaccgggga tgatgccaaa
     3841 attggtcatc acttgaaacg ccttgtccac acgctggcgc agttcaggcg gaaggtcatc
     3901 aaggttttgg taggtctggc cgttggcgtt gatgttgaca tgggtggagt tgaccgccat
     3961 cactttttgc accagatcgc cttccataaa atcaggaatg ccattcccgt ttttgtctgc
     4021 aaagatgccc atcatctgtt cataagccag acgttctttt tcgggcatct cctcaaggct
     4081 gttgtagctt tttccgttaa atatgatcgt tgccattgaa actcctttac tctggctggc
     4141 ctttcatcaa ggcaatttta tacggatata aaaccgcgcg catcaccccg gcgccgacag
     4201 ctgggtctga ctccatgatc gtcctcgccg ctgattcatc ctccgcctca aaaatgcaaa
     4261 ttccaaacgt ggattcgtcg ttgttctgtg tgcgtcccac catgatcatc acgccgttgc
     4321 ggctgagttc ctgcaaatga gcaaagtggc gcgaaatgac ctcggcttct tcgggcgttg
     4381 gcccatcggt gaccatttca agacgggatg gtttaagttg gtaaagccat tgctgcatgg
     4441 ataccttcct tttgttacat atactttgtc atcatgtcag ttcttgcatc gccgacaagc
     4501 gcaacctttg tcttgttgga tgcatcgtat cggaccgtga cttcactgcc ttcgcgcagc
     4561 atcagggttt gagtgaggcg gcatttcatc tgggcttcga aggctgaccc attggcaggt
     4621 tggacacgca ccggcaaatc cagccagatg tcactgcctt tgtagccgcc gatccctgtg
     4681 atcgcattgt tcttaatgat aatcgctttc gcctcggtgc cgctttgcaa gacctgcctg
     4741 acccacaaag gctggatcat tcccatgatc gagtacccga tgttgatcac gagcatcaac
     4801 acggccaacc caagcaaaat gccgcccaga gtggcaaacc cgggtgtgtt gaccaaaagt
     4861 atgccgggca gaaagaataa caaaaaacca gcaaagaaac caagcataac catggcgcgt
     4921 tttcgcattt gtcttttctc ctaaaatttt tgagatcgat caggggatga cctgcccgta
     4981 aaattcgacg aatccacatt ggtcgcaaca aaacgccacc aaccccttgc gtgccgccga
     5041 cattgggctt gcagaaaaca ttttcgatag atcgggtcct ggctcaccat ctggcgcaaa
     5101 gacatataga tagcggttgt gtgttgtgta ctcgttgtac ttcatgattc gcccctgtgt
     5161 cattgcccct ttgcattttg gacatacttt ttcatcggtc atttgggtct ccttttgaat
     5221 gtccccattc taatgacccg aaaagaaaat caatatgcct caaaagtatg aactacccct
     5281 gcagcgtctc ccactctgcc agtttctcgt ccatttcctt ctgtgtggat tcgtaagcct
     5341 tgctcagttt caccacttcg cccgcatcag atggcggatt ttccaactga ctgccgatat
     5401 cggccagttg tttctccaac tcggcaatgc ggttttcaag ctcctgcaac tgcgcgatct
     5461 ttcttctttc ctcttttgtc ttgacgttcg aaggcgcgcg tttgacattc gaggtggaac
     5521 tcgtttcacc tgccccttgt gccgccatga aagccagata ctcctgcttt tcccgttcgg
     5581 cgcggagttg tgaataggtg ccattgaagg cggtcagatg tgagccatcc acatcaattt
     5641 cccagatctg ggtggcgacg gcatccacca gataacggtc gtgggtgatg agcaggatcg
     5701 tccccgcgta atcttccaaa accgcttcca aaacttcctg cgacggaata tcgaggtggt
     5761 tggtgggttc gtcaagcaaa agcaggtttg tatcctgcaa ggccagtttc gccaatgcca
     5821 gccttccgcg ctcaccaccg gagagcatat ccactttttt gtacacatcg tcgccgctga
     5881 aaaggtactt gcccaaataa tcgcggatct gtcccggcag ccaatgcggc atgaccgaat
     5941 cgatctcctg caacaccgtg cggttcgggt cgagaccttc gtgcgcctga gcaaaatacc
     6001 caatttgcag actcgctccc agaaccactt caccagccaa cggttcgagt tgaccgagga
     6061 tcgtcttcag aaatgtcgtc ttgcctgcgc cgtttggtcc gatcaaagcc gcgcaatcct
     6121 gccgacgcag ttcgatatcg ggagtcttga aaagcatttt gcccgggtag cccacttcca
     6181 gatcttttgt gcgcaccacc agatcacccg aacgtttttc agaaccgagg cgcaaatgca
     6241 gatgcggcag cgtacgcaca ggcgagcgca aggcgcgcac acggcgttcg gcttcttcca
     6301 cgccaaagtt cgatgtggtc acatgcacat cttcggcggt ctcgctccac ttttgattca
     6361 acgccgcttc aaaaccgatc tgttccacag cctgcacgat gcgcgaaagc cgcttcaatt
     6421 ttccttttgc ctgctgggtg ttctgccccg aaatattgcg cttgatgtat tcaacttcct
     6481 tgagcagttt ttctttttca ctttcgaaaa cttcctgcct gcgcgtgatc cgttcttcgc
     6541 gctgtttcag gtaggcggtg taattgccgt tgtaaatttc gtatgcgccg ggcagcattt
     6601 ccaaaatgac attcgaggct ttatccaaaa aataacggtc gtgcgaaacg atcaccgccg
     6661 cgccttccca ttgactcaaa tatccttcga gccattcaac agcgcggatg tcgaggtgat
     6721 tggtcggctc atcgagcagg agcacatcgg ggtccgaaag aaggagacgc gataaaaagg
     6781 cacgggtacg ctgcccaccg gaaagatgat ccaaagaaag gctgtagtct tcggcgtcga
     6841 atcccaaccc ggtcaacacc tgtttgatgc gcgtctcgta ggtgtatcca ccgcgcctct
     6901 cgaagtcttc ctgtagtttc ccatatcgtt cgatcacatc ggggttggtc gccatctggc
     6961 tttcgagttt gtgcaactct tcctgttttt ggatcaattc ctcgaaaacg gaatgacacg
     7021 catcccagag agtcccatcc attttgaaat ctgcctcttg cgacaaatac ccgattcgga
     7081 ctcccctgtt tctggatacg tttcccgtcg aagcatcatc ctccccgacg atgatccgca
     7141 gcagggttgt tttgccaacg ccattgggac cgaccaaccc caaccgtgcg cccttctcnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cacattgctg agattgggat
     7321 gcatactgtc cttgcgcagg atgccttcgc attgcatccc tgctttttcc atcacccgcc
     7381 gcgaagggat attttccacg tcagtggtgg cgtacacgcg gaagatcgtc ggctgttcga
     7441 atgcccacac aatcgaggcg catacggctt cggtcatgta gcctttgccc cattgggcga
     7501 atgcccctgt gtaaccgaag ctcatcttgt aaggttccat gcgaagctcg atcatgcctg
     7561 ccagcgcatg gctttccctg atctccagca aatagcggaa attcgtttcc ccatcccacg
     7621 ctgccatgga tcgtttcatc agttcctgtg tctgttccac gcaggtgtgt ggacgccatg
     7681 ttagaaaacg ggtgacttcg ggatgcctgg cccagccttc gaacacggct ggagcatcgt
     7741 ccatgcgtgg tttgcgcagg gtgagtcttt cagtgatgat gacctcaggt ggtttcataa
     7801 atcctttttt gtggtgcggg atattatctt gccctgtcag tttcctgcct ttgcattctc
     7861 tttagcgcca ataaaccagc gatattgaaa atcacagcca gcaatgccac aggcacaaac
     7921 ccgaaacgct ggtaaatgat tgcggcaagg aatgcaccga gcgcacgtcc tacggcatga
     7981 cctgtcacat tcagtgagag catggtggcg cgcgccgatg ggacaagctc agtcatcatg
     8041 gggatgtgac tgaccatgat gtattcaaag gtgatataga agaagaacag cccgatcaac
     8101 gcgccgatct gggttgttcc aatgatcggg agcaggagcg aggcaacagc attgccgacc
     8161 agaccaatgg tcagcgcgag cggtttgccg agtctatcgg tggtgagagc gacgagtccc
     8221 tctccgctca attctgagat gccgatcaca gcagacgccc ctgcaagcgc ggcgatcttc
     8281 agcccaaatg aatcttcgag ccagacgccg aagatcacat tgaccagttc attgcccgca
     8341 ctggcccaca tggcgatcga aatccctgcc aacgcaggga ttgatttgaa gaccgcactg
     8401 taaccttcgc gtgaattggc aacaggctca tgatgttcat cttcatgcgg aatgatgcgc
     8461 cagatgatga agaatacaag ggcgccaagt gcagcgagga cagggaaggg tgccgaccaa
     8521 ctgaatttcg agatcaggaa tcccatcgca ggaacaccga tgatgaacgc catggaccag
     8581 gccatctccg tgacagcaag ggcggtgccg cgttgttcgt agggaatgcg atcgccgaaa
     8641 taggcttgca tggcggggtc gaacatgtac tttcccagca cggcaaggat gagtgctgcg
     8701 gcaagcgtcc aatacgatgg gtgaatggcg acgatgccca tgccaagcgt gaagatggag
     8761 atgcctgcca gcatgccaaa cttgcgtcca cgccgatcgg cgatgggtgc aaagagcggg
     8821 cttgccgagc cgatcaggga acgggctgtc atcaacaggg acatggaggc aatatccacg
     8881 ccgaggccgc gcgcaaatac cgataagaat ggatacacca tcctgtgggc ggtgttcagg
     8941 atggtgcgga ggaatacaaa gattgtgagt tgtaggcgta ttttcaaaat ataaactgta
     9001 gtgcaagata ttatcctgca ctacctttct agttggcctt gagttgcaaa aaccattttg
     9061 caaggaagta ggctgccagc catgcgagca ggttcaagcc gacgaagaac ccgatgatga
     9121 cgtaattgcc tgaattgaag atcggcgatg gcggcgggtg catgatgttt tgtgcagtgg
     9181 aaaagatcac ccgcagaaca acaatgaaaa taatgttgcc cagtgaagtg agccatgaaa
     9241 tattgaacaa ccacaagcca gcctgaagca gaatgcccgt aatggcaaag accatggcca
     9301 gacggaagcg aggttcagcg agaaataatc cgttccagtt ggcttgcatg gcaaagaggg
     9361 aaagcggcag gtaggtgagc caataaacaa tgccggtgcg gccaagagcg gcactccagg
     9421 cgtggagctg gtcatttttc aggagcagtc ctaaaagtcc agtaagagcc gcagccgcga
     9481 aggcaagttc agcggtcaat acccaggcgc cgtgcaggta cacgatccgc acgtttgatc
     9541 ccagtgattg ttcttcgggg ccaaataagg ccagcaaggc gattgccccg atggtaagta
     9601 gaaaaaaaag caggggtgat tttgttttta acatgtcacg gattttaaca tcctgaacga
     9661 tgtttttata gtgccttttg tcagttccat gcggttaccc attgagataa aattcatacc
     9721 tggtttgaaa tctaatgaag tggaaggttc tttattaaca tggaatactg gcctttgatc
     9781 gtgatcgttc ttcagttggt gtttttggaa gggattctat ccattgacaa tgccgcagtg
     9841 atcggtgccc tggtttcccc actgcctgag aatgaggatg tgccctggcc gggaaagctg
     9901 aaaaaactcg ggacagtttt acatcctttc cttggttttc aaaggatggc tgcgctgcgg
     9961 gtgggtttgt tgggggccta tttgggacgc ggcgcaatgc tgtttttgac gagttttttg
    10021 atccacaact catggatcaa attgatcggt gccgtgtatt tgatccacct tgcgtttgac
    10081 aaccttgaag atatgacggg tgatagcgag gaggaaggcg ggctttctcc gatcaaggtg
    10141 cgctcatttt gggccaccgt gatgaccgtg gagatcatgg atctcatatt cagcattgat
    10201 aatgtggttg cggctgtatc gctttccgat aagatctggg tggtgatgtt gggggtgggg
    10261 atcggcattt tgacaatgcg ttacgcggcg ggtattttta gttatgcagt gcagcatgaa
    10321 ccgatcctga agcaggcggc ctatattctt gtcttgaaca tcggcgtgga gttgattttg
    10381 gatcaggtct ggcatgttga aattcccgat ttgttgcgtt ttggaatttc agttgcaacg
    10441 atcgcgcttg ccattgccta tgctcatttt ccttttttgc agaagtttcg ctttgtgctg
    10501 gtctggctgg cgaaaggcat cggcatcgtc aacgaatttg tggattggtt gttcatcccc
    10561 tttaaagaat tgttcaatct tattgccagc ctttttgtgc gccctgcagg tacccaccct
    10621 gccgagtgac cttgaaatct gtatataaac ataacccgcc ctgcgaaagt ttgatttcgg
    10681 ggcgggttat gtttttgtct gtcattttca ggaagataat tccttttcca tccacaccag
    10741 gtgagtggtg cccaaatgcg gggtgggaat tcctgcaata tggtagccgt atttttcaaa
    10801 atatgctgtg gctgtaacac gtgccattgc gccaagggtc ttgaatccat gttcgtgggc
    10861 gcgtttttca atttccacga tcagaaattt tccgatgcct ttgtgctgat gttcctgcgc
    10921 aacggccaaa tgagagacct gacccacccc gggcgctttt tcaaacagtt tgatcacacc
    10981 aagaacttcc cccgtctttt cgtccacagc catgaaatgc tcgctgatgg gctcgtaatc
    11041 gtctttttca gtgccttttg ggtgccccca tggttcgcga agaaccttgt aacgtaacga
    11101 atagtaggcc ttgaactcct cgcgggtttt tggcgttttt atgattggca tattgccctc
    11161 cgcaacaaac tttctcaatt gactgggtca ttatactgct caatcatggc gcccgaacaa
    11221 aactgttgac tcttttcctt gacagtgact tttaccatgg agtacactgg ctaaggttgg
    11281 ggagtagcca cctgttcccc acaggatgga tcgtcgtcaa accattggtg taacatccac
    11341 catcggcggt tcgtgcgcaa attttgtgcg ttggcgagac caccacgaat tgattcgtgg
    11401 tggtcttttt ttatcttcat ttcaggagac gagtgatggc tatcgaaaaa aaggaagaca
    11461 tgcttcagga aaatgcctgg catgcattga atgccgagca ggttctgcgt gacctggagg
    11521 ttcatgaaga gggactgacg agtgccgaag cggagaagcg ccttgcgcat tatggtccga
    11581 atcagttgcg cgaagcaccg cggcctggct tccttgccct gctgtgggga cagttgaata
    11641 attttgttgt gattcttttg attgtcgcct cggtgatctc tgccctgctg ggcgattata
    11701 ttgaagctgc cgcgatcatg gcgatcgtgg ttttgaatgc tgtccttggt attgtgcagg
    11761 agcagcgcgc tgagcaggcc ctggccgctc tcaagaaact ggctgcgccc gatgcgcgcg
    11821 tcctccgtga tggtgcgcat acctctgtcc cttcttataa ccttgtgccg ggggatatcg
    11881 tctttcttga agcagggaat ttcattcccg ccgacttgcg cctgcttgaa gccgtcaact
    11941 tgcgtgtgga ggaggcatcc ctgaccggtg agtcgcttcc tgtgcagaag aatgccgcca
    12001 ctgtgcttga taaaaatgtg ccgcttggcg atagaaaaaa caccgccttt atgggcaccg
    12061 tggtcaatta cgggcgtgga cgtggtgtgg tcaccagcac aggcatgcgc acccagcttg
    12121 gcctcatcgc caccatgctt cagaacgttg aaaccgagga gacgccgctc caacgcaggc
    12181 tcgaccagct tggacgttcg ttgagcatcg gctcgttgat ccttgtggcg gttgttttta
    12241 tcgttgcact cttcaactat accgagatca gcggattgtt ctcttcgccg ttggattacc
    12301 tcaaggaatt cgcctctgaa atcacagagg tattcatcat cgccatcagc cttgcgattg
    12361 ccgccgtacc ggaagggctc cccgccgtgg tgacgatctc gcttgcgctg ggcatgcggg
    12421 aaatggtaca acgccacgcc cttatccgaa aactgtcctc cgttgagacg cttggttcgg
    12481 ccacgacgat ttgttccgac aagaccggaa ccctgaccca aaatgaaatg accgttaccc
    12541 gtgtgtgggc agataatcaa ttcgtccaca taacaggcac cgggtatgcc ccgaacggtg
    12601 aatttcagat ggatggaaag actgtggatg tgacgaaata ccccgcgatc ctttctgcgc
    12661 tgtggcttgg tattttgaac aatgatgcca ccatcgaaac gaccggtgaa accgaatcgc
    12721 agaggaccta tcgcattgtg ggtgacccaa ccgaaggtgc tttgttggtg gcggctgcca
    12781 aggctggggc gatccacgtt gatattgatg aagcctaccc gcgtgagaac gaagtgccat
    12841 ttgattcgga acgcaaacgc atgatcacca ttcacgatgt gcgcagtccg aaaccgactg
    12901 acccatcccc gttttacgat gaaaagcata aagattggga cgtgatcgcc gtcaaaggtg
    12961 cgcccgatat tgtgctggaa ctttgcacca aatacctcgg catggacgac aagccgcgtg
    13021 aaatgacaaa ggaagcgaaa gagcgcgtcc tcgctgccaa cgatgccatg accaaagatg
    13081 ccctgcgcgt acttggtttt gcatatcgcg tggaaaaaga tgtgcccgat aaccctgaaa
    13141 atatcaaagc cgatgaactc gaaaaggacc tggtcttcgt gggtctgctt ggcatgatcg
    13201 acccgccgcg taccgaggtg aagcccgcac tcgaaaaagc ccgccatgca ggcatccgca
    13261 cggtgatgat caccggcgat ttcccgaata ccgccaaggc cattgccgaa tcgatcagcc
    13321 tgctgcgccc cggtaagaat gtaatgaccg gtgctcaact cgatgccatt gatgataacg
    13381 aactcaagag gatcatcgaa gataccgatg tctttgcccg tgtatccccc gaacacaaga
    13441 tgcgcatcgt ggatgccttg caggccaatg acgagatcgt cgccatgacc ggtgacggtg
    13501 tgaacgatgc tcctgccatc aagcgcgccg acattggtgt ttcaatgggc attacaggga
    13561 ccgatgtagc caaggaaaca gccgatatgg tgctcaccga tgataactac gccagcatcg
    13621 tttcagcagt ggagcaggga cgcatcatct atagcaatat ccgcaaattc gtgttcttcc
    13681 tgctttcctc caacgtggct gagatcatga tcattttcct tgccactctg gcaggcctgc
    13741 ccgcaccatt gaccgcgatc caattgctct ggctcaacct catcaccgat ggcgcgcctg
    13801 cactggcact cgccatggaa aaaggcgatc ccgatatcat ggatcagaaa ccgcgcgcca
    13861 agaacgaacc gatcgtcaac cgttcgatgg gcatcggcat catcgtgcag accatcgcac
    13921 agacgagcgc cgtgctcggg gcttttgtca tggggttggc gtggcacctc gaagcaggag
    13981 cagttctccc gtcggggatg aacgtgttgt ccttcgtcct ctcacatgac tggcgcggtg
    14041 tggatgttca aaccgcagag acgatggcct tcgttacgtt gtcgctggct gagttgttcc
    14101 gtgcgtacac cgtccgctcg gagcgtgctt ccattttcca gatcgggttc ttttccaaca
    14161 agtacatgca gtatgcggtg ggcatttcca ttgcgctgct gttgctggtg tgtgccgtgc
    14221 ccttcctgca gcccattttt aacacccact tcctcagcgg gcgtgaatgg gggctggtgc
    14281 ttgggttggc gctcatgcct gccgtggctg aagagatcac aaaattcttc ctgcgaagaa
    14341 gcagggcata aaaaacgaga gtggctaagt ccgtgggcta atcacaaaga cgccaagcca
    14401 cgaagcccca agaatccttt gtgccttcgt ggcaatcaaa gcccattctt ttagacactc
    14461 cctaaaaaaa caaaatcctc ctcgaaaaaa atcggggagg attttttgat taaaaagtga
    14521 agtatgattc agccatgttc agatttcaag gcgaaccatt tacccctgag cagaaaacga
    14581 aatttcattt ttttgcctcc atccttgcgc tggcattgat cgccgtggtc gtggccgttt
    14641 tatttcaacc cttgattctg ccgcgcatgg atttttacaa cgagctttgg gggccttcat
    14701 acctgctcgt acacagacaa agcccatacg ataccgccag cctgaaccct gtgttgcctg
    14761 ctgtctggtt cccaatggca attggattct tctttccgct tggctggctt tctgaagatg
    14821 cggccttgca ggcctggttc tttttcaata ttgttgagat ttgcatcgtc gtttttctgg
    14881 tgcagggaaa agaccgctcg ttgtacaaca cggcagcact ggccttgcta tgtttctttt
    14941 tcccctttgt cttgaatcat ttcaacctcg ggcagttttc catcaccatt ctattgtgct
    15001 tgctgctttc agcgcattgg atcggaaaat ataaaaactg gccttcggcg tttttccttg
    15061 cgcttgccct tgccaagccg caattgggga ttctggctgt gattggtctg gctttgcatc
    15121 aattccgcca acgtggattc gcagggatgt ttttgttcgg cgcacagacc ttgctcatgg
    15181 ctctgttgat gagcctgccc ctgttcatcg cccaccctgc atggattccc gactggattg
    15241 caagcatggc aaaaaattac acctggcagc acccttcgtt gttttcggtt tttcagcaaa
    15301 tgctgggaag atgggggctg gtgccttggg cggcaattgc agcgggtgtg ctggcattca
    15361 catttttatt gtggaaaaag ataccgccct ttgcagccat gctatggagc cttgcgctga
    15421 caacgcttgt cactccctat gtctggagtt gggattttgt cctgctcctg ccgttgtgga
    15481 tgttttcgtt tacaaaggca aattggaaat ggaaaatatt gttattggtt tcatacctta
    15541 ttgcctggta tggcatggca ctggtgcaaa tgcaggcagg gagtcagaac cagtctttct
    15601 ggtgggtgcc tttgtggttt atcggcacac ttgtgccagc ctggcgaatg ggtgaagtaa
    15661 aatatttcga ctcatgacga ttttattttt tacaaaagga tgaccatatg ccgcgtttta
    15721 tccgtgtgac tttttttctc gcgcttcttt ccctcaccct ggcctgtgga ctcttccaat
    15781 cggccccgtc ggtgcgtgat cctttgtggg tttacgccaa cccgaatttc tcccccgatt
    15841 acgcctccat tcaatcttct gagagcggac tcgtcaccta tcaaagcggc gcttccaacg
    15901 aactgccgat ctcgctcatt accctcaacc cagccagcgg agaggaaatc ttcacgccca
    15961 cccccaacga tgagttggtc atttttgaag gagttgccgc gggtagggcc atttttgttg
    16021 gccgagtcag tgatcaggtc aatgaagacg gcgatgttct ctatcccatc attgcgttgg
    16081 atccctcaac aggtctggaa gtatggcgat acgatggcag cgtgtttgaa agcggaattc
    16141 cgcgcacgga aaaatatctt tttgtttcaa aacgctattc ccagatcgat gtggtggatt
    16201 tgatgaacgg ggaagtggtt gccagtttta cgaaggaata cgatgccgcc gaactcttcg
    16261 acctcaaccc aggctggatt cggtatatgt acaccgatac tgccatttat tcgctcagcc
    16321 cggcgggagt gctgcgccgt tatgacctgc ccacattaac cctgcaatcc acacttaacc
    16381 ttgatatacc gcattacctt gatgcgttct tcattgaagg agatcgactc tacgtatatt
    16441 ccgcagaggt tctggatgct gatttggacc tgcttgcctt cgaccttgcc agcggcacat
    16501 acctgtggga attgcgcggg atgcgcggtt ccacggtggg cctggagttt tacaacggct
    16561 tcgggtacgt ggatacggtc aacggcccga gcaaaataga cctttccaac ggacaggttc
    16621 tttggtcggt ttctgttcaa ttggcgccct ttttcatcgg tgatgattca aatggcgttt
    16681 ttctggccac acaggatgga cttctcactg cgtacgattc caccagcgga aaggaactgt
    16741 ggacttacag aacaggccag cccaactcga tcaacctcag cgcagtgaat ggggtggcgt
    16801 ttatcaccac tgccgaagac ctggctgcgt tcgaatcctt ttttgttccc aatcaacttg
    16861 atgcggtgga tattgccaca ggcaaggcac tctggcgtta caaaaaccca tacgtgaccc
    16921 tgcccatcgg cacggacaat gccttcatcg tcgggtatta cggtggcatc accgccctgc
    16981 ctgcaaaata aaatccccgc tctttttgag gtgtgatgtc agggcgtagg aaaaaaataa
    17041 agacttctag ggcttccatt ttcgcaggaa ggcgtgcatg tccttgaggg tttgtctcca
    17101 gtctgattcg gcgctgtttt ttcgttgaat atcctgtgcg cgctgattca tcgctgcgat
    17161 cacgcgctca tcctcgcccg cgtccagcca gaagccgtga tcgttttcgg cacaataatc
    17221 cagtttcagg tcgtgcaggc ggtattgaaa ttcatccatg gctgcgccgc aatgcggaca
    17281 ggggaattct gttttggctt ggtggtggat caacgagccc ttgcgggaat cgtcatcgaa
    17341 ggccacatct tcaagtttgt ccagttcgtt aaagtccagc cacatgccgc ggcagttggg
    17401 gcaggaatcc acttcgatca tgcctttgta gtattttttg gcgagttctg tgttgcattt
    17461 tgggcagttc attttattgt tttctccagc atggaaagag aaaaatgtct attttctcaa
    17521 gccgccgttg ggggtgttat tcagtaaggt ttgatgtact ttcagccatc gcttcatcca
    17581 ccagatcttc aaccgatctt ccattttcct gaaaatcgag ccagaccagg aattcggcat
    17641 tgcccttcgg ccccagcaaa ggtgacttga tcaacccgcg caggttgaaa ccttcctgtt
    17701 ttgcgaacgt caacacatcc agcaaaacct gtctgtgaat ctcagggtcg cggatgacac
    17761 catcgccgcg ggccacatct tttttacccg cttcgaattg gggtttgatg agggcaacga
    17821 tcccttcttt cttcttttct ctttcatcgt cattgaaaga agaaagagga aagaaagaga
    17881 accatttttt gaccaccggc agcaatatct tcaacgaaat gaatgaagca tccaccgtca
    17941 ccaaagacac cgcctcaggc agcgactcga catgccgtgc attggtctct tccatgacaa
    18001 ccacacgggc gtagttgcgc aatttccaat gcaggatgcc cttgcccaca tcaatggcgt
    18061 agactttggc cgctccgcgt tgcagcaggc agtcggtgaa tccgccagtg gatgcgccga
    18121 catctgcgca gatgaaatct ttcacgtcaa ttccgaatct ctccagcgcc gcatccaatt
    18181 tttcgccgcc acgtgaaaca aagcgcggac cggtatccac cgtcagcgct gattttgtgt
    18241 ctatcgctgt ggcaggtttc agggtcactt gatcgttcac gcgcacttgt cccgccatga
    18301 taagggcctg agctttcgcg cgcgattcgg ccaatccgcg ctccacgagc aaaacatcca
    18361 atcgaat
//
LOCUS       DAOQLC010000090        18074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1085_length_18074_cov_20.962484, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000090 DAOQLC010000000
VERSION     DAOQLC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1085_length_18074_cov_20.962484"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PL000_14885"
     CDS             <1..473
                     /locus_tag="PL000_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90222.1"
                     /translation="RAARILLVARFLCIIPHMLHPIWKFESDLTSRLLFWSILSIFSG
                     LFIWFAADGFGRGFGIQAVVWGLIDAAIAIVGARTAARRKSTADPKREAIFIRKVLWI
                     NFGLDVLYILGGVWLIQNSVDGFWRGSGWGIVLQGAFLFFFDLFHAIKIPVELK"
     gene            470..970
                     /locus_tag="PL000_14890"
     CDS             470..970
                     /locus_tag="PL000_14890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HNC90223.1"
                     /translation="MTFSFLNRKCKAREHPEGGCGVFATDFISKGELISLWGGDIVHR
                     SKLDPDMPRFTQRVLQIEEDLFILTSAAPEPNDCFNHSCDPNLGFTGQIGLVAMRDIS
                     AGEELCFDYAMSDGGPYDEFDCYCGSPLCRKKVTGNDWKLPELWVKYDGYFSSYLAKR
                     IEKIRK"
     gene            967..1581
                     /locus_tag="PL000_14895"
     CDS             967..1581
                     /locus_tag="PL000_14895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNC90224.1"
                     /translation="MKYLAGERLKFDWRVVSVTIISTLLLMVDSYHKLTPYKYWDRII
                     LYLAIPMLVISIFFFEDPREYGFSFGDWKLGLAYTILGILLMAPIIYYLGHGNPVMRA
                     YYADYLPGLPWTTFLDLLGWEFFFRGWILFAYARRFGHEALWLQAVPFALAHISKPEV
                     ETLSTIFGGFAFGWVAWRTRSFVWPFLIHWFIATFVILVAAGAL"
     gene            1583..2026
                     /locus_tag="PL000_14900"
     CDS             1583..2026
                     /locus_tag="PL000_14900"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90225.1"
                     /translation="MSKESIRPALESESAQIKDLINLVGINPTGLDWKRFVVMVNDGG
                     QVIACGQIKPHGTDIRELASIAVHPDHRGKGLARAVIEVLLEQTERPVYLMCIAHNGP
                     MYEKFGFHTVSDDQMPKYFKRIKNLFDVAVLFRKTEENLLVMKLE"
     gene            2036..2605
                     /locus_tag="PL000_14905"
     CDS             2036..2605
                     /locus_tag="PL000_14905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNC90226.1"
                     /translation="MKNVLYILLGIVAGFLLAGALIFVSRAPAGEPIVLRAAPTAAPI
                     KVHVIGAVPRPDVYDLPEGARVQDAINAAGGLLADANVDGLNLAALVVDGQQLSIPYK
                     DGAEPVETVELPGSETATPDPNAGEKININTATLEELDTLPGIGLTTAQKIIDYRLAN
                     GPFAKIEDIMNVSGIGPTTFEGLKDLITV"
     gene            2605..3153
                     /locus_tag="PL000_14910"
     CDS             2605..3153
                     /locus_tag="PL000_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC90227.1"
                     /translation="MPFDHFAAIAPLYARVTYSKREIMREVAALPVKGRLLDVGGGTG
                     RVSSAIRDLVDEVVIADVSMGMLKETLPTPLRPVCGGSESLPFADESFERVIMVDALH
                     HVINHAESAREMFRVLKTGGVLVIEEPDIRTFGVKLIALAEKLLLMRSHFLAPDKIEK
                     LFEIGEKKIRAEDGTAWVIVRK"
     gene            complement(3215..4366)
                     /locus_tag="PL000_14915"
     CDS             complement(3215..4366)
                     /locus_tag="PL000_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC90228.1"
                     /translation="MDFDLNEEQKAWKKAVHDFVGKVVKPKSREVDETESFNWEAVRK
                     GGPLGLMGLSIPEAYGGSGVDMVSSSIAMEELGWGCGSTALAFTAHNGLGTAPIALYG
                     SEELKKKWLPLVAEGKNKLGALALTEPGAGSDIQGGVITRAEKQGDEWVINGAKMWCT
                     NASIAEYIITLVRTDPKGGSKSLSMILVPTDSKGLTIGPPEKKMGLRGSPACGISYDN
                     VRVPLGNLVGAEGRGLQQTLATLDAGRISIGAISVGLAQAALEAAVDYARERKAFGQS
                     IAEFQAIQFKLSNMATEIELARTMIHKAAWTKDQGRPFNKEAAMAKLYATEMAERVCY
                     DAIQIHGGNGYSREYNVERMYRDARLMTIGEGTSEIQRLVIARHVLASE"
     gene            complement(4390..5208)
                     /locus_tag="PL000_14920"
     CDS             complement(4390..5208)
                     /locus_tag="PL000_14920"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HNC90229.1"
                     /translation="MQQYFKKQIALPQDHGSWVFIFSPLLIGVFAGKHFTYATFNLII
                     AAMSAFMIRQPMTVIVKTISGRRPKTDLPAARFWLLVYGGIATLALLGLILEGFAYIL
                     YLAIPGAPVFAWHLWLVSKRSERRQSGVEIIATGVLALVAPAALWVGLGHYDPAGWWL
                     WVWAWLQSAASIVYAYLRLEQREQVEGQERSALWKAGRRAFLYTSFNLILSLLLGWAG
                     LIPQLIFAAFLVQWLETLWGITHPATGWKPVKIGMRQLIVSILWTVLFIVFNHA"
     gene            5305..5997
                     /locus_tag="PL000_14925"
     CDS             5305..5997
                     /locus_tag="PL000_14925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC90230.1"
                     /translation="MAKKKNVKNAREAKSDRTIQYVVVALALIVLGFIGWPYLPKPKS
                     NVAVGTTPACEVFDSIAAAEQYTSAPPLSIDESKPYFATFKMEKGGEFVVQLYSDKAP
                     VTVNNFVFLACKGFYNGVTFHRVMDGFMAQGGDPTGSGMGGPGYSFEDEISDLTFDRE
                     GLLAMANSGPNTNGSQFFITYAPTDWLNGLHTIFGEVVSGMDVVNAITRRDPQQNPGV
                     EGDVIESIIITQ"
     gene            6105..6347
                     /locus_tag="PL000_14930"
     CDS             6105..6347
                     /locus_tag="PL000_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90231.1"
                     /translation="MTETFTKGESHGCIMCGKLYQLYVVTDSKGKFVDAKVMSAGGRL
                     VKHPQRALVACETHSNAEIEAAVARAYGVQKEDDDD"
     gene            6428..8011
                     /locus_tag="PL000_14935"
     CDS             6428..8011
                     /locus_tag="PL000_14935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNC90232.1"
                     /translation="MSSTSKFILAIDLGTSGPKVALFSTQGELIGSEFQETPVILLPN
                     GGAEQSPAGWWSAIDTACKRLLAKKLVSSDDIVAIANTTQWSGTVAVDADGNAIENAV
                     IWMDSRGAPYIKKIADGFPKIEGYGLSKVLNWIRLTGGAPGNSGKDPLAHILYLKDVR
                     PDIYQRTYKFLEPMDYIGLLLTGEFAASFNSITLHWMTDNRDIQNVKYHDGLIQLAGI
                     DRAKLPDLKPANAHLGTLRREIASAWGLRDDVKVFMGSPDLHSAAVGSGAVRDFEPHL
                     YVGTSGWLVCHVPFKKTDIFHNLAALPSGIPGRYLLTNEQESAGGCLQYLRNNILFPQ
                     DELSVGEKPANAYQLLDQMAERTPAGSGKLIFTPWLYGERTPVEDHLVRGGFHNQSLS
                     TTRADMVRAVFEGVAYNARWLLQYVEQFNKRPVESINMVGGGAKSNIWCQIHADVLNR
                     PIRQVKDPIEANVRGAALLASAGMGYLKYDEIGTHVKIAATYVPNPDNRKIYDELFRE
                     FLAIYESNRKIHARLNRMH"
     gene            8067..9569
                     /locus_tag="PL000_14940"
     CDS             8067..9569
                     /locus_tag="PL000_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC90233.1"
                     /translation="MSWMDTLMKQAGKLNPRVLAFLEKRMKNIPSVAKKIEGEYAEIM
                     TELETSVKPYKNKFTAFSQIPAKGRPHADILNEMEAMRALEESHWKDGFISGAVYHGD
                     QAHIDFLNQVYALNSQSNPLHADVWPSATKFEAEIVSMTAAMLGGAGVDACGTLSSGG
                     TESIMLAMKTYRDWAREVKGITRPEMIVPVTAHAAFDKASQYFNIKTIHVPVDENFRA
                     DLKAVKKAISKNTIVIVGSAPSFPHGAIDPIAEMSELARQHNIGFHTDACLGGFVLPW
                     AEKLGYDVPAFDFRLAGVTSISVDTHKYGYAAKGTSVILYRTQELRRFQYYTTTEWPG
                     GLYFSPTFAGSRPGALSAACWAALTSIGEEGYLAATKRILETAEKIKQGIREIPELYV
                     LGDPFFVVAFASESLNVYKVLDHMAHKKWSLNGLHKPTCVHLCVTLRHTQPGVAERFL
                     TDLREAVEHVKGHPEEKGSMAPVYGMAATIPMRGMVSDLLKKYLDLIFKV"
     gene            complement(9654..10955)
                     /locus_tag="PL000_14945"
     CDS             complement(9654..10955)
                     /locus_tag="PL000_14945"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNC90234.1"
                     /translation="MTFPLPSLIAVSAANPWTYILFGVIGFAFGYTLEMSGFGDSRKL
                     AAQFYFTELTVLKVMFTAIVTAMVLLFGAVGLGILDFSQVWVNPTYLWSGLVGGLIMG
                     VGFIIGGFCPTTSLASASTGKIDGMMFMLGGFVGAALFAESEPLFTNWYNNAGYFGRV
                     TLDEVFGISKGAVVVLVILMALFMFWGSEQLERIFGKKDLSKEPKIRVAGAAVLAVMA
                     FAVFFIGSPSLEDKYNKLTFTRTETIEQLNADPIVETKIYTADEMLSNRLVFVSPAEA
                     FKTKYAQTVNPVYLDVRPEADFNLYHLEDAVNVPLDRLTEVIPVLLTEPPANTVYILM
                     SNDEAAAVQAWKTLVASSVPNVYILEGGINNWIAFFGKDDKALQPNPNAGDDQLGYTF
                     PAALGSRYDSCSPNPQEYEELDFEAKIKLQLKRDKSGGGCG"
     gene            complement(11042..11587)
                     /locus_tag="PL000_14950"
     CDS             complement(11042..11587)
                     /locus_tag="PL000_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNC90235.1"
                     /translation="MTTQTKKSIFSRPEKSYVHPYFGGIVLGIVLFLALFLTGNGLGS
                     SGATARIDAAIVDAVSPSHVDNNPYLLKMAGGDKNPFDDWIVPVFVGALLGGFSSGFF
                     NGRLKFMTTKGPNISNQTRWLMAFLGGVIFLYGARMARGCTSGQALTGGTVLSAGSWV
                     VMFAIFGGAYGLAYFVRKLWN"
     gene            complement(11610..14297)
                     /locus_tag="PL000_14955"
     CDS             complement(11610..14297)
                     /locus_tag="PL000_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNC90236.1"
                     /translation="MNKYKLLTVVGFLVLVLAIGIGTVFASPQPAPTQQESILHPNFI
                     LLDKNGVNVIENNSPVSTTQTCGQCHDTAFIESHAFHADLGLADYQETKDLNASNGLF
                     GEWDPLTYRYLSQDGDERLDLSTAEWLMLNGDRVVGGGPATTSRNGIALTDVKANVEN
                     PETAILNNGTAATWNWDESGTMEMNCFLCHLASPNTEARSEAIHAGEFGNANTATLLN
                     LNIVTTSADGWAWNAEAFNENGELKSEVLGIQDPTNENCAACHGEVHPNSGEPLTVNQ
                     CDLEYPQTATTGQVVSGQRINASGVNLTGKNDLDRSWDIHAERQLQCTDCHYALNNPA
                     HASELQSSNPEHLIYDPRSLEINEYLQRPDHNFARGESAQFNVSPEYKGTMRRCENCH
                     DASKGHSDWLPYIDTHMEAVACETCHIPQMYAPAIQAYDWTVITTNGNAVSSCRGVEG
                     TPNEVTTLVTGYEPVLLNRTNGDGNTLLAPYNLITSFYWVYDDANGNKRPVRLVDLET
                     AYLDNGNYASDIITAFDTNNDGKLSTAELKIDSSAKEEAVKAKLLGLGLNNPRIEGMV
                     QPYSINHNVTRGEYAVNDCKACHDADSRMTQPIKLADNAPVMPVFDGNNNVNGTGDLV
                     KGADGTLYYQPNPAKDNMYVFGTSRVNWVDWFGAIAFAGSLLGVIGHGTLRIFSSRKQ
                     VKGHVRTERVYMYEPYRRFWHWLQTSAIVLLLFTGLIIHRPDIFGAFSFRGVVTVHNV
                     LAVILVINAAMSLFYHLATERIQEFIPRPYGFFDDAMVQAKYYINGIFKGEGHPFEKL
                     PTSRMNPIQKATYFAILNVLLPLQIITGALMWAVQKWPEIAGGLPFLAPFHSLVAWMF
                     GTFIIVHVYMTTTGATPLEATRAMITGYEEVEVHTHNEG"
     gene            complement(14365..15801)
                     /locus_tag="PL000_14960"
     CDS             complement(14365..15801)
                     /locus_tag="PL000_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNC90237.1"
                     /translation="MKKRFPLWTIGLVGIILLILVPVLYFLPRTQSKNDPAAHLPTKP
                     VHVDHKDIVKGEFKTGQDVTRACLECHEDAATEFMKTTHWTWESKAFDVPWREEDVTI
                     GKINQINNFCIGSQGNENKCMSCHAGYGWEQGKEQQQSNEENVDCLACHADTGSYGKG
                     LFGNPAEGVDLLAAAQSVRAPTRENCGKCHFDGGGGNGVKHGDLDESLYFPTQNNDVH
                     MGGDLNMQCTDCHWTQDHQILGRMIADNYTIDPKEQVSCEQCHVEQKHEDERINTHLS
                     AVACQTCHIPALALEDPTKVYWDWSQAGQEGRTDDHFTYLKIKGEFIYDKNFAPAYMW
                     FNGNNEYRYILGDPLGQDGITYINKPAGSIDDKSAKIFPFKLHVAKQPYDVNNNYLLQ
                     PITSGKDGFWTTFDWNSAFELAAPITGLEYSGQYDFTTTYMYWATTHMVQPADQALQC
                     DSCHGENGRLDWEALGYPGDPIEWGGRQ"
     gene            complement(15798..16145)
                     /locus_tag="PL000_14965"
     CDS             complement(15798..16145)
                     /locus_tag="PL000_14965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC90238.1"
                     /translation="MVTPTLQQEITQLEANFCAALSDPNRLLILYALNDKPMNVTELG
                     NELGLNQPTTSRHLKVLRERGLVVAVRMGTTITYHLSDPRLIQALDLLRSVMRERLAY
                     QASLINEISQENV"
     gene            16329..16940
                     /locus_tag="PL000_14970"
     CDS             16329..16940
                     /locus_tag="PL000_14970"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNC90239.1"
                     /translation="MEVFQREASSVDVATSLRELREARGISMRTLATKSGLSANALSM
                     IERGKTSPSVSTLYKLADALGVSITAFFGTETEKKQVVFLKQDERTRMSFTRGLFEAL
                     GGEQFVGRVEPFMLTLESGASSGPHSIVHSGHEFVFCLRGQLDYTVEKQVFHLTVGDS
                     LLFASRLQHKWKNPGHTVTNALIVISGFAEGEQPHSMHWKKGE"
     gene            complement(17027..17371)
                     /locus_tag="PL000_14975"
     CDS             complement(17027..17371)
                     /locus_tag="PL000_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HNC90240.1"
                     /translation="MSEETKEIQWTIHKTHPEMVKNARDRLSEVVDPEIGMNIIQLGL
                     VRDIEIENNIAHLKMILTTPFCPYGPAMIEMTKAKATEGLNMPVNIEMGMDVWDFSMM
                     EDPSALDWGMYA"
     gene            complement(17381..18010)
                     /locus_tag="PL000_14980"
     CDS             complement(17381..18010)
                     /locus_tag="PL000_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNC90241.1"
                     /translation="MKSTRDKILQTLLKKPRSTINDLAEAVGINPISVRHHLTNLQME
                     GLVEGQEERHGVGRPRLVYVLTSEGMEHFPTRYMLLTTRLLSQMKETMPGPVVAKLFN
                     QIAEDLASQYANDMKHLSMEERLDFVKDMLAQEGFTVEWEKKDGNYQIHEISCPYYQI
                     GVAHPEVCTVDQTLISKMLALPANKVQCILDGGAHCTYVVQPVVEVKSK"
BASE COUNT         4128 a         4192 c         5031 g         4723 t
ORIGIN      
        1 ggcgagcggc gaggattctc cttgtcgctc gttttctttg tataatccct catatgctgc
       61 accccatctg gaaatttgaa tcggacctaa ccagccgcct gcttttctgg agcattctct
      121 cgatcttttc tggacttttc atctggttcg ccgccgatgg atttggccgc ggctttggca
      181 ttcaagcggt tgtgtgggga ttgatcgatg cagcgattgc cattgtcggc gcaaggactg
      241 ccgctcggcg caaatcaaca gccgacccga agcgtgaagc gattttcatc cgcaaagtgt
      301 tgtggatcaa cttcggtctg gatgtgttgt acatcctcgg cggtgtctgg ctcattcaaa
      361 attcggttga tggattttgg cggggcagcg gatggggaat tgttctacaa ggggcattcc
      421 tctttttctt tgatcttttt catgcaataa aaattcctgt ggagttgaaa tgacgttttc
      481 ttttctcaac cgaaaatgca aggcgcgcga gcaccccgag ggcgggtgcg gcgtttttgc
      541 caccgacttt atttcgaaag gcgagttgat ctcattgtgg ggcggtgaca ttgtccatcg
      601 ctcaaaactc gaccctgaca tgccgcgttt tacacagcgc gttttgcaga tcgaggaaga
      661 ccttttcatt ttgacatcag cggcccccga accaaacgat tgcttcaatc attcctgtga
      721 tccgaacctg ggtttcaccg ggcagatcgg cctggtggca atgcgtgata tcagcgcggg
      781 ggaagaattg tgctttgatt acgccatgtc tgatggcggg ccgtacgatg aattcgactg
      841 ttattgcggc tctcctcttt gccgcaaaaa agtgacaggc aacgattgga agctgcctga
      901 attatgggtg aaatacgacg gttatttttc ctcctacctt gcaaaacgca ttgaaaaaat
      961 caggaaatga aatatctggc tggcgaaaga cttaaatttg attggagggt tgtcagtgtt
     1021 acgatcatct ccacattgct tttgatggtg gattcgtatc acaaattgac gccctacaaa
     1081 tattgggatc gcatcatcct gtatcttgcg attccaatgt tggtgatttc gattttcttt
     1141 tttgaagatc caagagagta tggcttttcc tttggcgatt ggaagctggg gcttgcctac
     1201 accattttgg ggattttgtt gatggcaccc atcatttatt atttgggtca tggtaaccca
     1261 gtgatgcggg cctattacgc cgattacctg cccggactgc catggacgac ctttctggac
     1321 cttttggggt gggagttttt ctttcgcggt tggatcttgt ttgcgtatgc gcgcaggttc
     1381 gggcatgaag ccttgtggtt gcaggcggtt ccgtttgcgc tggctcatat cagcaagccc
     1441 gaagtggaaa cactttccac gatctttggc ggatttgcct ttggctgggt ggcgtggcgc
     1501 acaaggtcat ttgtctggcc gttcctgatc cactggttca ttgccacgtt tgtcattctt
     1561 gttgctgcgg gagcgttgtg aaatgagtaa agaatcgatt cgtcctgcgt tggagtcaga
     1621 atctgcccaa attaaggacc tgatcaattt ggtcggaatc aacccgacgg ggttggattg
     1681 gaagcgtttt gtcgtgatgg tgaatgacgg tgggcaggtc attgcctgcg gtcagatcaa
     1741 gccgcacggg accgacatcc gcgaactggc ttcgattgcg gtgcatcccg accaccgtgg
     1801 aaaagggctg gcgcgtgcgg tgatcgaggt attgctggag cagaccgagc ggcctgtgta
     1861 tttgatgtgt attgcccata acggcccgat gtatgaaaaa ttcgggtttc atacagtatc
     1921 cgatgaccaa atgccaaaat atttcaaacg catcaagaat ctattcgatg ttgcggtgtt
     1981 gtttcgaaaa acggaagaaa acttgctcgt catgaagttg gagtaaaatc gtctcatgaa
     2041 aaacgtttta tatattctgc ttggtatcgt ggctggattt ctgctggcgg gtgcgttgat
     2101 ctttgtttcg cgtgctcctg ctggtgagcc gatcgttttg cgcgcggcgc ctacggcggc
     2161 acccatcaaa gtgcatgtga tcggagccgt gccgcgcccg gatgtgtatg acctgcccga
     2221 aggcgcccgt gttcaggatg ccatcaatgc cgcaggcggt ttgctggccg atgcaaatgt
     2281 agacggcttg aatcttgctg cactggttgt ggatggccag cagttgagca ttccgtacaa
     2341 agatggtgcc gaacctgtgg agactgttga attgccaggc tctgaaaccg caacacccga
     2401 cccgaacgcg ggtgagaaga tcaacatcaa caccgccacc cttgaagaac tggatactct
     2461 gcccggcatc ggtttgacca cagcgcagaa aatcattgat taccgtctcg ccaatggccc
     2521 gtttgcaaag atcgaagata tcatgaacgt ttcaggcatt ggcccaacta cttttgaagg
     2581 tctcaaagac ctgatcaccg tctaatgccc ttcgatcatt ttgcggcgat tgccccgctt
     2641 tatgcgcggg tgacttattc aaaacgtgag atcatgcgtg aggtggctgc gctccctgtg
     2701 aagggacgcc tgcttgatgt gggcggcggc acggggcggg tttcttctgc cattcgtgat
     2761 ctggtggatg aagtggtgat cgccgatgtt tccatgggga tgttgaaaga gacgcttccg
     2821 actccgctgc ggcctgtgtg cggcggttct gaatcattgc ccttcgccga cgaatctttt
     2881 gaacgcgtca ttatggtgga tgcattgcat catgtcatta accatgctga aagtgcgcgt
     2941 gaaatgttcc gtgtgttgaa gacgggtggt gtgctggtga tcgaggagcc tgatatccgc
     3001 acctttggcg tgaagttgat cgcgcttgca gaaaagctgc tgctgatgcg gagtcacttc
     3061 cttgcgccag ataaaataga aaagttgttt gaaattgggg aaaagaagat cagggcagaa
     3121 gatggcactg cctgggtaat tgtccgaaaa taaagtttga ttccagatgt atcaaaacgt
     3181 tgaccgccga ctgcagtcgg cggtcaaatg tacgttattc acttgccaat acatggcggg
     3241 cgatgaccag tctttggatc tcgcttgtgc cttcaccgat ggtcatcagg cgggcgtcgc
     3301 ggtacattct ttccacgttg tactcgcggc tgtatccatt tccaccatgg atttgaatgg
     3361 cgtcatagca aacgcgctca gccatttcgg tggcgtagag ttttgccatg gcggcttcct
     3421 tgttgaatgg cctgccctga tcctttgtcc acgcggcttt gtggatcatg gtgcgggcca
     3481 gttcgatctc ggtggccata ttcgataatt tgaactgaat cgcctgaaat tctgcaatgc
     3541 tttgaccaaa tgctttgcgc tcacgggcat aatccacggc ggcttcaagc gcggcttgtg
     3601 ccaacccaac ggaaatggca ccgatgctga tacgccctgc gtcgagtgta gccagagtct
     3661 gttgcaggcc acgaccttct gcaccaacga ggtttccaag cggcacgcgt acattgtcgt
     3721 agctgattcc gcaggcgggt gatccgcgca gccccatttt cttttcgggc gggccaatgg
     3781 tcagtccctt ggaatcggtg gggacaagga tcatgctcaa tgattttgag ccgcctttcg
     3841 ggtcggtgcg tacaagggtg atgatgtatt cggcgatgct ggcattggta caccacatct
     3901 ttgcgccgtt gatgacccat tcatcgccct gcttttcggc acgggtgatc acgccgcctt
     3961 gaatatcaga ccccgccccg ggctcggtca gcgcgagtgc gccaagttta tttttgcctt
     4021 cagccacgag cggcagccat ttctttttca attcttcgct gccatacaag gcgatcggcg
     4081 cagtgccgag gccattgtgg gcggtgaacg ccaatgcagt ggaaccacag ccccagccca
     4141 actcttccat tgcaatggaa gaggaaacca tatccacgcc tgagccgccg tatgcctcag
     4201 ggatgctcag gcccatcaac cccaacggac ctcccttgcg aaccgcctcc caattaaacg
     4261 actcggtctc atccacttca cgtgacttcg gtttgaccac tttgcccaca aaatcgtgga
     4321 cggctttttt ccatgctttt tgttcttcgt ttagatcaaa atccattttt tctcctgtga
     4381 gttatttggt caggcatgat tgaagacgat gaaaaggact gtccacaaaa tgctgacgat
     4441 gagttgacgc atgccgatct ttactggctt ccaacctgtg gcagggtggg tgatgcccca
     4501 aagcgtttcg agccattgca caaggaatgc ggcaaagatc aattggggaa ttaaacccgc
     4561 ccagccaagc agcaatgaca ggatcaaatt gaaggatgtg tacaggaagg cccgtctgcc
     4621 cgccttccac agtgcgcttc tttcctgacc ttcaacctgt tcccgctgct caagcctgag
     4681 atatgcgtag acgatgctcg cggcagactg aagccatgcc caaacccaga gccaccagcc
     4741 cgctgggtca taatggccaa gtcccaccca aagggctgcg ggagccacaa gggcaaggac
     4801 acccgttgca atgatttcca cgcctgattg tctgcgctcg gaacgtttgc ttacaagcca
     4861 caagtgccac gcaaagacgg gtgcgcctgg aatggcgagg tataaaatgt aagcaaaccc
     4921 ttcaaggata agtccgagta aagccagggt ggcgattccc ccgtagacga gcaaccagaa
     4981 acgggcggca ggaagatccg tttttgggcg gcggcccgag atcgttttga cgatgactgt
     5041 catcggttgg cgaatcataa atgcagacat cgccgcaatg atgagattga aggtggcgta
     5101 ggtgaaatgt ttccccgcga aaaccccgat caatagtggg ctgaagatga acacccatga
     5161 tccgtggtct tgcgggaggg cgatctgctt tttgaagtat tgctgcatat ttggcaagca
     5221 tacaagagaa ttcgcctgtg cgcaaggttg ttacaccatg ggtgttatat aattggaaca
     5281 aattcaattg aggagagttc aggaatggca aaaaagaaaa atgtaaaaaa cgccagagag
     5341 gcaaaatctg atcggaccat tcaatatgtg gtggtggctt tggctttgat cgtgcttggg
     5401 ttcattgggt ggccgtacct tccgaagcca aaatccaatg ttgcggtcgg caccacgccc
     5461 gcttgtgaag ttttcgattc cattgccgct gccgaacaat atacatctgc gccgccgttg
     5521 agcattgatg agtcgaagcc ttattttgcg accttcaaaa tggagaaggg cggtgaattt
     5581 gttgtgcagt tgtattctga taaagccccg gttacagtga ataattttgt gttccttgcc
     5641 tgcaagggct tttacaatgg tgtgactttc catcgtgtaa tggatggctt tatggcgcag
     5701 ggcggcgacc cgacaggctc aggcatgggc gggcccgggt attcctttga agatgaaatt
     5761 agcgacctga catttgacag ggaagggctg ctggccatgg ccaattctgg gccgaacacc
     5821 aatggcagcc agttctttat tacctatgcc cccacagatt ggttaaatgg attgcacacc
     5881 atttttggtg aagtggtcag cggtatggat gtggtgaatg ccatcacgcg ccgcgacccg
     5941 cagcaaaacc ctggtgtgga aggcgatgtc atcgagagta ttatcattac gcaataaaat
     6001 tgctggaagc gcagactaca gtctgctttt tcaatagcgt caccattgaa caggctggag
     6061 ttctctctgg cctgttttgt tttgaaaacg aaaaggagtt tgttatgaca gagacattca
     6121 ccaaaggtga atcacatggc tgtatcatgt gtgggaaact gtaccagctt tatgtggtca
     6181 ctgattcgaa ggggaaattt gtagatgcca aggtaatgag cgcaggcggg aggctggtga
     6241 aacaccctca acgggcgctt gtagcctgtg aaacccacag caatgccgag atcgaagccg
     6301 cagtggctcg ggcgtatgga gttcaaaaag aggacgatga cgattgaccg atttatggtt
     6361 ttttcgcatg ggttggtgta tcatcgccgt gttttgatgc agtcacaact cctaaactcg
     6421 aggccaaatg tcatccacaa gtaaattcat tctcgccatt gacctgggta cgtcgggtcc
     6481 caaggttgca ttgttttcca cgcaaggtga attgatcgga agcgaatttc aagaaacgcc
     6541 tgtcattctt ttgccgaatg gcggcgcgga acagtcacct gctggttggt ggagcgcaat
     6601 tgataccgcc tgcaaacgtc tgcttgcaaa gaagctggtt tccagtgatg acattgttgc
     6661 catcgcaaat actacacaat ggtcgggcac ggtcgcagtg gatgcagatg gaaatgccat
     6721 tgaaaatgcc gtcatctgga tggattctcg tggcgcacct tatatcaaaa agatcgcaga
     6781 tggtttcccg aaaattgaag ggtacggact ctcgaaggtc cttaattgga ttcgcctgac
     6841 gggcggcgcg ccgggaaact cgggcaagga tccgcttgcg catattttgt acctcaaaga
     6901 tgtccgcccc gatatttatc aacgcacata taaatttctc gagccgatgg attatatcgg
     6961 tctgctgctt actggcgaat tcgccgcatc gttcaactcg atcacgctgc actggatgac
     7021 ggacaatcgc gacattcaaa atgtgaaata tcatgacgga ttgatccagt tggcggggat
     7081 tgaccgcgcg aaacttcccg acctgaaacc tgccaatgcg cacttgggca ctctgcgccg
     7141 tgagatcgcc agtgcatggg gcctgcgcga tgacgtaaaa gtattcatgg gttcgcctga
     7201 tctgcactcg gcggctgttg gttctggcgc cgtacgtgat tttgagccgc atttatacgt
     7261 tggaacatca ggttggttgg tatgccatgt ccccttcaaa aagaccgaca tttttcataa
     7321 cctggccgcg ctgccatcgg gaatccctgg gcgatatttg ctcaccaacg aacaggaatc
     7381 agctggtgga tgcttgcaat acctgcggaa caacatcctg tttccgcagg atgaattgtc
     7441 tgtgggcgag aaacctgcca atgcttatca attactcgac caaatggcag aacgcacccc
     7501 ggcaggcagt ggaaaactga tcttcactcc ctggctatat ggcgagcgga ctcctgtgga
     7561 agatcatctt gtgcgcggcg gattccacaa ccagtcactc agcaccaccc gcgccgacat
     7621 ggtacgggct gtctttgaag gtgtggctta caatgcgcgc tggctgttgc agtatgtgga
     7681 gcagttcaac aagcggcctg tcgaatccat caacatggtg gggggcggtg caaaatcgaa
     7741 tatctggtgc cagatccatg cggatgtgtt gaatcgcccc atccgtcagg tgaaggaccc
     7801 gatcgaagcc aatgtgcgtg gcgcagccct gcttgcttct gcagggatgg gttatttgaa
     7861 atacgatgag atcggcacac atgtgaaaat tgcggccacg tatgtgccca accctgataa
     7921 tcgaaaaatc tatgatgagt tgtttcgtga attccttgcg atctacgaaa gcaatcgcaa
     7981 gatccatgcc cgtttgaatc gaatgcatta atctatatgg tagaggcaca gcaacgctgt
     8041 gcctctacca tcatctggag aagaatatga gttggatgga tacactgatg aagcaggcgg
     8101 ggaaattaaa tccgcgggtg ctggcgtttt tggaaaaacg catgaagaat attcccagcg
     8161 tcgcgaaaaa gatcgaaggc gagtacgccg agatcatgac cgagttggaa acttcggtaa
     8221 agccttacaa aaacaaattc acggcgtttt cccagatccc tgccaaggga cgacctcacg
     8281 ccgatatcct gaacgaaatg gaagcgatgc gtgccctgga ggaatctcac tggaaggacg
     8341 gctttatctc tggggcggtc tatcacggtg atcaggcgca tattgatttt ctgaatcaag
     8401 tgtatgcgct taactcgcag agtaatccgc tccatgcaga tgtgtggcca agcgccacca
     8461 agtttgaggc agagatcgtt tcaatgactg cggccatgct tggcggggca ggtgtggatg
     8521 cctgtggaac gctttcctct ggcggcacgg aaagcatcat gctggcgatg aagacctatc
     8581 gtgattgggc gcgtgaggtg aaggggatta cccggcctga aatgatcgtc cctgtaacgg
     8641 cacacgctgc tttcgacaaa gccagtcagt actttaatat caagaccatt cacgtgcctg
     8701 tggatgaaaa ctttcgcgct gacttgaagg cagtgaaaaa agcgatctcg aagaatacga
     8761 tcgtcattgt agggtcggca ccttcgtttc cgcatggcgc cattgacccg atcgcagaga
     8821 tgtctgaatt ggcgcgtcag cacaacattg gtttccacac cgatgcctgc ctcggcggtt
     8881 ttgttttgcc ttgggctgaa aaattgggat atgacgtccc agcctttgat tttcgcctgg
     8941 caggtgtgac gtccatttca gtggatacgc ataaatatgg gtacgctgcc aaagggacct
     9001 cggtcattct ctatcgcact caggaattgc ggcgctttca atattacaca accactgaat
     9061 ggcctggcgg tttatatttt tccccgacct ttgctggcag ccgtcccggt gccttgagcg
     9121 cggcttgttg ggcggcattg acttccattg gtgaagaagg gtatctggct gcgacgaaga
     9181 gaatccttga aaccgcagaa aagatcaaac aaggcattcg agaaattccc gaattgtacg
     9241 ttttgggcga tccgttcttt gtggttgctt ttgcctcaga atcgctcaat gtgtataaag
     9301 tgctggacca tatggcacac aaaaagtgga gtttgaatgg cttgcacaaa cccacctgtg
     9361 tgcatttgtg tgtcactttg cggcataccc aacccggtgt ggcagagaga ttccttacgg
     9421 acctgcgcga ggcggttgag catgtcaagg ggcacccgga ggaaaagggt tccatggctc
     9481 ctgtctacgg catggcggcg accattccca tgcgagggat ggtcagtgac ttgttgaaga
     9541 agtatttgga tttgattttc aaagtgtagg caggaaagaa gaaagaggaa agaataaaga
     9601 aatcgcgcga atgatctcgc gcgatttctt tatttgattg ctgaaaaccg ttcttatccg
     9661 cacccaccgc cagatttatc acgtttcaac tgtaacttga tcttggcttc gaaatccaac
     9721 tcttcgtatt cctgcgggtt ggggctgcat gaatcgtagc ggctgcccaa agctgcgggg
     9781 aaggtgtagc ccaattggtc gtcaccagca ttcgggttgg gttgaagcgc tttgtcatcc
     9841 ttgccgaaga atgcgatcca gttgttgatt ccgccttcga ggatgtacac attcggcaca
     9901 ctggaggcga cgagggtctt ccatgcctgc acagcggcgg cttcgtcgtt gctcatcaaa
     9961 atgtagacgg tgtttgcggg tggctcggtg agcaaaacgg gaatgacttc ggtgagacgg
    10021 tccagcggta cgttgacggc gtcttcaaga tgatagagat tgaagtcggc ctcggggcgt
    10081 acatcaagat agacggggtt gacggtttgg gcgtacttgg tcttgaaggc ctctgctggg
    10141 ctgacaaata cgagccgatt ggaaagcatt tcatcggcgg tgtagatttt tgtttcgacg
    10201 atgggatctg cgttcaattg ctcgatggtt tctgtgcgtg tgaaggtgag cttgttgtat
    10261 ttatcttcga gcgatgggct gccgatgaag aagactgcga aggccatgac ggcaagcact
    10321 gcagcacccg caacgcggat tttaggttcc ttgctcaggt cttttttgcc gaagatgcgt
    10381 tccagctgtt cggaacccca gaacatgaac aatgccatga ggatgacgag gacgacaacc
    10441 gcacctttgg agatgccaaa gacctcgtcg agggtgacgc gcccaaagta gcctgcgttg
    10501 ttgtaccagt tggtgaagag cggttcgctt tcagcgaaaa gagccgcgcc gacaaaacca
    10561 ccgagcatga acatcatgcc gtcaattttg cctgtggagg cagatgccag tgaagttgtg
    10621 ggacaaaaac cgccaatgat aaaaccaacg cccatgatga gcccgccgac aaggccagac
    10681 caaagatagg tggggttgac ccaaacctgg ctgaaatcaa gaattccgag gccgaccgct
    10741 ccgaaaagca agaccatggc ggtgacgatg gcggtgaaca tgaccttgag cacggtcaat
    10801 tcagtgaaat aaaattgtgc cgcaagtttg cgtgagtcac caaaaccaga catttcgagg
    10861 gtgtaaccga atgcaaagcc aatgacaccg aagagaatat atgtccacgg gttggctgcg
    10921 ctgactgcaa tgagagaggg gagagggaat gtcattttgt ttctcctgtg tgtgagttga
    10981 aggttttagg ttgaaggttg caggttattc ttcaacctgt aacttacaac ctgcaactct
    11041 tttagttcca aagttttctg acaaagtatg ccagcccata cgcaccgcca aagatggcaa
    11101 acatcaccac ccaggagcca gccgaaagca ccgtgccgcc tgtgagtgcc tgccccgatg
    11161 tgcatccacg ggccatgcgc gcaccgtaga gaaagatcac accgccaaga aaggccatca
    11221 accagcgggt ttggtttgaa atgttggggc ctttggtggt catgaatttc aagcggccat
    11281 tgaaaaagcc tgaagagaat ccgccgagta aagcgccgac gaaaactggc acgatccagt
    11341 catcaaaggg gtttttgtca ccgcctgcca ttttcaacag gtaggggttg ttgtccacgt
    11401 gagatgggga gactgcatct acaatggcgg cgtcaatgcg ggcggttgcg cctgatgagc
    11461 caaggccatt gcctgtcaga aagagcgcca ggaacaggac gatgccaagc acaattccgc
    11521 caaagtatgg atgcacatac gatttttcag gccgactgaa gattgatttt tttgtttgag
    11581 tagtcatggt tttattcatc ctttatcttt catccttcgt tgtgcgtatg cacttccact
    11641 tcttcgtagc cagtgatcat ggcgcgggtt gcttcgagcg gtgttgcacc agtggtggtc
    11701 atgtacacat ggacgataat gaacgtgccg aacatccacg caacgagcga gtggaacgga
    11761 gcaaggaagg gaagtccgcc agcaatttcg ggccattttt gcacagccca cattaatgcc
    11821 cctgtgatga tctgcagagg cagaagcaca ttcaaaatgg caaagtaggt ggctttctga
    11881 attgggttca tgcggctggt cggcagtttc tcaaatgggt gcccttcgcc cttgaagatg
    11941 ccgttgatgt aatactttgc ctgcaccatg gcgtcatcga agaagccata agggcgcggg
    12001 atgaattcct gaatacgttc ggtggcaagg tggtaaaaga gcgacatggc cgcattgatg
    12061 acgaggatga ccgcaagtac attgtgtacc gtcaccacac cgcggaatga gaatgctcca
    12121 aagatatctg gcctgtggat gatgagacca gtgaagagca ggagaacgat ggcagatgtc
    12181 tggagccaat gccagaagcg acgatacggt tcgtacatgt aaacccgctc ggttcttacg
    12241 tgacccttaa cctgtttccg cgacgaaaag attctcagcg tcccatgtcc gatcacacct
    12301 aaaagcgagc cagcgaaggc gattgcccca aaccaatcca cccaatttac gcggctggtg
    12361 ccgaaaacgt acatattgtc tttggcggga ttgggctggt aataaagcgt gccatcggcg
    12421 cctttgacca gatcgccagt gccgttgaca ttgttgttgc catcgaatac tggcatgacg
    12481 ggtgcattgt ccgcaagttt gatgggttgg gtcatgcgtg aatctgcgtc gtgacaggct
    12541 ttgcagtcat tgactgcata ttcgccgcgg gtcacgttgt ggttgatgct gtagggctgc
    12601 accatacctt cgatccgcgg attgttcaga ccaagcccga gcagttttgc ttttacggct
    12661 tcttcctttg cggaagagtc gatcttcagt tcggcagtgc tcaatttgcc gtcgttgttt
    12721 gtgtcgaaag cagtgatgat gtcgctggcg tagttgccgt tgtcgaggta ggctgtttcg
    12781 aggtccacaa ggcggacggg gcgtttgttg ccattggcgt catcgtacac ccagtagaac
    12841 gaagtgatga gattgtacgg agccagcagg gtattgccat cgccgttggt tcgattgagc
    12901 aatacgggtt cataccctgt aacgagggtt gttacttcat tgggagtgcc ttccacaccg
    12961 cggcaggagg aaacagcgtt gccgttggtg gtgatgacgg tccagtcata ggcctgaatg
    13021 gcaggggcgt acatctgcgg gatgtgacag gtttcgcagg cgacagcttc catgtgggtg
    13081 tcgatgtagg ggagccaatc tgaatggcct ttgctggcgt cgtggcagtt ttcacagcgg
    13141 cgcatcgtgc ctttgtattc gggagagaca ttgaattgtg cgctttcgcc gcgtgcgaaa
    13201 ttatggtcag ggcgctgcag gtattcgttg atctccaaag agcgcgggtc atagatcaga
    13261 tgctcagggt tgctgctttg cagttcgctg gcgtgagcag ggttgttcaa tgcgtagtga
    13321 cagtcggtgc attgcaactg gcgttccgcg tggatatccc atgagcggtc gagatcgttc
    13381 ttgcctgtca ggtttacgcc tgaggcatta atgcgctgtc cgcttacgac ctggcctgtg
    13441 gttgcagtct gtgggtattc gaggtcacat tgatttacgg taagcggctc accagaattc
    13501 ggatgaacct ctccgtgaca ggcggcgcaa ttttcattgg tggggtcttg aattccaagg
    13561 acttcgctct tgagttcgcc gttttcgttg aaggcttcgg cattccaagc ccagccatca
    13621 gcagatgtgg tgacgatatt taggttgagc agtgtggctg tgttggcgtt gccaaactca
    13681 cctgcatgga tcgcttcgga gcgggcttcg gtattgggtg atgcaagatg gcagaggaag
    13741 cagttcattt ccatggtgcc cgattcgtcc caattccagg tggcggcagt accgttgttc
    13801 agaatggctg tttcagggtt ttcaacgttg gctttgacat cggtcaatgc aatgccattg
    13861 cgggatgtag tggctggtcc gccaccgaca acacggtctc cgttgagcat cagccattca
    13921 gcggtggaga ggtcaaggcg ttcatcacca tcctgtgaaa gatagcggta ggttagcgga
    13981 tcccattcac cgaagaggcc gttgctggcg ttgaggtctt tggtttcctg ataatctgca
    14041 agcccaaggt ctgcatggaa agcatggctt tcaataaatg cggtgtcatg gcattgtcca
    14101 caggtttggg tggtggagac tgggctgttg ttttcgatca cattcacgcc gtttttatcg
    14161 agcaggataa agtttgggtg aaggatgctc tcttgttggg tgggagcagg ctgaggcgaa
    14221 gcaaagaccg ttccaattcc aatggcaagg acaaggacta gaaatccgac tacggtgaga
    14281 agtttatatt tgttcataat tcatccttgt ggattaggaa ttaggaatta agtgattctt
    14341 aattcctaat tcacaattcc tgatttattg ccttcctccc cattcgatcg ggtcgccagg
    14401 gtagccgagg gcttcccagt cgaggcggcc attttcgcca tggcaggaat cgcattgtag
    14461 agcctggtca gcgggctgca ccatgtgggt tgttgcccag tacatgtagg tggtggtgaa
    14521 gtcgtattgt ccgctgtatt cgagacctgt gatgggggct gccagttcaa atgcactgtt
    14581 ccaatcaaag gtggtccaga agccgtcttt ccctgaggtg atgggttgga gcaggtaatt
    14641 gttgtttaca tcgtagggtt gtttggcaac atgtagtttg aaggggaata ttttcgcgga
    14701 tttgtcatcg atgctgcccg caggtttgtt gatgtaggta atgccatcct gcccgagcgg
    14761 gtcgcccaaa atgtagcggt attcattgtt gccgttgaac cacatgtagg caggtgcgaa
    14821 attcttgtcg tagatgaatt cacccttgat cttgagatag gtgaagtgat cgtctgtgcg
    14881 gccctcctgc cctgcttgag accagtccca gtagactttg gtggggtctt caagcgcgag
    14941 tgcgggaatg tggcaggttt ggcaggctac ggcagagagg tgggtgttga tgcgttcatc
    15001 ttcatgcttt tgctctacat ggcattgttc acaggagacc tgttccttgg gatcgatcgt
    15061 gtaattgtcg gcgatcattc tccctaagat ttgatggtct tgtgtccagt ggcagtcggt
    15121 gcattgcatg ttcaagtcac cgcccatgtg gacgtcattg ttttgggtgg ggaagtacag
    15181 gctttcatca aggtcgccgt gtttgacgcc gtttccgccg cctccatcaa aatggcattt
    15241 gccgcagttt tcgcgtgttg gagctcggac gctttgagca gcggctaaaa ggtcaacacc
    15301 ttcggcggga ttgccaaaca gacctttgcc gtaggagcct gtatcagcgt ggcatgcgag
    15361 acaatccacg ttttcttcgt tggactgttg ttgttccttg ccttgctccc agccataacc
    15421 tgcatgacag gacatgcatt tgttttcgtt tccttgtgag ccaatgcaga agttgttgat
    15481 ctggttgatc ttgccgatcg tgacatcttc ttcgcgccag ggcacatcaa aggcttttga
    15541 ttcccatgtc caatgcgtgg tcttcataaa ttcagttgcc gcatcttcgt gacattccag
    15601 gcaggctcgg gtcacatctt gtcccgtttt gaactcacct ttgacaatgt ctttgtgatc
    15661 cacgtgtaca ggctttgttg gtaggtgtgc agcaggatca ttttttgatt gggtgcgtgg
    15721 gaggaagtaa aggacaggga cgaggatcaa caaaatgatg ccgaccagac ctatcgtcca
    15781 gagtgggaag cgttttttca tacattctcc tgtgaaattt cattgatcag gctggcttgg
    15841 taggcaagtc gctcgcgcat cacacttcgc aacaggtcaa gcgcctgaat taatcttgga
    15901 tcggataaat ggtaggtgat tgtggtaccc atgcggacgg caaccaccag ccctcgttcg
    15961 cgcaggacct tgagatggcg cgacgtagtg ggctgattca acccgagttc atttcccagc
    16021 tcggtgacgt tcatgggctt atcgttgagg gcatatagaa ttagtaagcg atttgggtct
    16081 gacagtgcag cgcaaaaatt agcttccagt tgggtgattt cttgttgaag ggtaggggtg
    16141 accatggtac ctgcctatat gcgaataaac gaatatttca atcccatcat aatattgtga
    16201 cagattgtac aatagtggtg tgtgtcatat taagtttcgt ttaatgtcac ataaagttag
    16261 gatggtgtat aattcgggca gatttggtag ggttacagtc ttcgatacca cgcaaaagga
    16321 gtttggaaat ggaagtattt caacgtgaag cgagttctgt agatgtggca acaagtttgc
    16381 gtgaacttcg agaggcgcgt ggtatctcaa tgcgtacact cgctacgaaa agcggattgt
    16441 cagccaacgc cttgagcatg atcgagcgtg gcaagacttc accttcggtg agtactttgt
    16501 ataaattggc agatgctttg ggggtttcaa ttactgcgtt ttttggtacc gaaacagaaa
    16561 agaaacaggt tgtttttttg aagcaggatg aacgcactcg tatgtcattt acgcggggtt
    16621 tgtttgaggc cttgggtggg gaacagtttg tcgggcgggt tgagccgttc atgctgactc
    16681 tggagagtgg agcctccagc ggaccgcata gcattgttca cagtgggcat gagtttgttt
    16741 tttgtcttcg tggtcagttg gactacaccg ttgaaaaaca agtcttccac ttgactgtgg
    16801 gtgacagtct tttgtttgcc tcccgtttgc agcataaatg gaaaaacccc gggcacacgg
    16861 ttacgaatgc tttgatcgtg atttctggtt tcgcagaagg ggaacagcct cattccatgc
    16921 actggaaaaa aggcgaatag ttcagatttg gatgataaag ataaaaaaat acgccaccaa
    16981 tttggtggcg tattttttgt aaaagacaaa tcaaacgact tgtaaattag gcgtacatgc
    17041 cccaatccaa tgcggagggg tcttccatca tggagaaatc ccacacatcc atacccattt
    17101 caatattgac tggcatgtta agcccttcgg tggctttggc cttggtcatc tcgatcatgg
    17161 ctgggccgta agggcagaaa ggggtggtca ggatcatttt gaggtgggca atgttgtttt
    17221 cgatctctat atcacgtacc aaacccaact gaatgatgtt catgccaatt tcagggtcta
    17281 ctacttcaga aagcctgtct cgggcgttct ttaccatttc aggatgggtt ttatggatgg
    17341 tccattgaat ttctttggtt tcttcgctca tggttcctct ttatttgctt ttaacttcaa
    17401 ctacaggttg gacgacataa gtgcagtgtg cgccgccatc gagaatgcat tggactttgt
    17461 tggcaggaag tgcaagcatt tttgagatca aggtttggtc aacggtgcaa acttcagggt
    17521 gggcaacacc aatctggtaa tacggacatg aaatttcatg gatctggtaa tttccatctt
    17581 tcttttccca ttccacggta aagccttcct gagccaacat gtctttgacg aagtcaagcc
    17641 gctcttccat actcaggtgt ttcatgtcgt tggcgtattg gctggcaaga tcttccgcaa
    17701 tctggttgaa taatttggca accacagggc caggcatcgt ttctttcatt tgggagagta
    17761 gtcgtgtagt caacagcata tagcgtgttg gaaagtgctc catgccttca gaggtgagga
    17821 cataaaccaa gcgcggacgc ccaaccccat gacgctcttc ctggccttcc accaatccct
    17881 ccatttggag gttggtaagg tggtggcgca cagaaattgg gttgatgcca acagcttctg
    17941 ccaggtcatt aatggtggac cgtggctttt ttagcaaggt ttgtaaaatc ttgtcgcgag
    18001 ttgatttcat gcatggcagt ataaccaaga ggcgagcgaa tgtcaatata ctagataaat
    18061 atcataaata tatg
//
LOCUS       DAOQLC010000091        18022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1092_length_18022_cov_26.296544, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000091 DAOQLC010000000
VERSION     DAOQLC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_18022_cov_26.296544"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..485
                     /gene="rpsU"
                     /locus_tag="PL000_14985"
     CDS             288..485
                     /gene="rpsU"
                     /locus_tag="PL000_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNC90242.1"
                     /translation="MATVNLRNGESQDSLLKRFRKKIVKSGILSTIRNKRWFVSKSET
                     RRMEKKKAIRRIKRRSFKDTE"
     gene            500..757
                     /gene="infA"
                     /locus_tag="PL000_14990"
     CDS             500..757
                     /gene="infA"
                     /locus_tag="PL000_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNC90243.1"
                     /translation="MTKEEGKIKVDGLVIEALPGTQFRVRLDNGHEVLAYLSGKMRKH
                     YIRILLGDHVSMEMSPYDLTRARITFRQRKNTPATPSTTGS"
     gene            complement(819..1820)
                     /locus_tag="PL000_14995"
     CDS             complement(819..1820)
                     /locus_tag="PL000_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HNC90244.1"
                     /translation="MTDLSTTYLGLKLKNPLVASASPLSKKVESAKKLQDAGISAIVM
                     YSLFEEQIIHESLELDHYLTRGTDSFAEALSYLPDSGMYSIGPEKYLSQLTGLKKALN
                     IPVIGSLNGVSKGGWTHYAKQIQDAGADALELNLYFISTDTALTSQELENAQIELVAE
                     IKSAITIPLAVKLSPFITALPNYARRIVEAGADGLVLFNRFYQPDFDLNELEIVHSLD
                     LSTSADLRLPLRWVSILHGKVNADFALTSGVHTSSDVLKAMMSGAKVAMMASKLLQTG
                     EQVIPSMLDELQAWMKEREYVSIEQMQGSMSQKSVKEPAAFERANYMKVLNSFRDLV"
     gene            complement(1817..5383)
                     /gene="nifJ"
                     /locus_tag="PL000_15000"
     CDS             complement(1817..5383)
                     /gene="nifJ"
                     /locus_tag="PL000_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNC90245.1"
                     /translation="MKQPIIMIDGNEATASVAHRLSEVIAIYPITPSSGMGEFSDEWS
                     AKGKKNLWGTTPLVIEMQSEGGAAGTVHGALQTGALTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARAIASHALSIYGDHQDVMAVRQTGWALLSSGSVQEAQDFAAIAQAAT
                     LKSRVPFMHFFDGFRTSHEVTKIFQLTDEELRQMVDDDLVRAHRARGLSPEHPTLRGT
                     AQNPDVYFQMREASNNYYAATPAIVQEMMDKFAKITGRHYNLFDYSGHPEAERVIIIM
                     GSGGEVVEETVNYLAKKNEKVGVLRVRLYRPFSIEHFLKGLPKNVKSIAVLDRTKEPG
                     ANGEPMYLDIVNALMEGNRSNIKVIGGRYGLSSKEFTPAMTKSVLNELAKPEPKNHFT
                     LGINDDVSNTSLSVDPSFSLESEGMVSCVFFGLGSDGTVGANKNSIKIIGEETENFAQ
                     GYFYYDSKKSGTVTMSHLRFGPKPIRAPYLIEANQANFVACHQYSFLERVDMLKYAKE
                     GGIFLLNSLYGPNEVWDQLPQEVQKDIITKKLKFHVINGYDVAEKTGMGGRMNTIMQT
                     AFFAISGILPRDAAIAEIKHAIEKSYGKRGEAVVRKNFEAVDATVANLYEVKVPAAVT
                     SKMTRRLPVPDEAPDFVKNVLGQIIDSDGDNIPVSAFPIDGTFPTGTTQWEKRNLALE
                     IPVWDADLCIQCGKCVMVCPHAVIRHKVYDEKMLADAPETFKHTASKFKEFSNGYAYT
                     VQVAPEDCTGCTLCVEVCPVKDKRAVGRRAINMEAQPPLREAEAKNWDFFMNIPDLDR
                     KLINPSTIKNSQLLRPLFEFSGACAGCGETPYVKLVSQLFGDRAIIANATGCSSIYGG
                     NLPTTPWAKNAEGRGPAWSNSLFEDNAEFGLGMRLTIDKQKEYARELLKSFETTVSTE
                     LVEAILNANQADEAGIGAQRERVAELKNKLSKSSDSRAKDLISLADNLVQKSVWIIGG
                     DGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQSSKSTPRAAVAKFAMSGKGM
                     PKKDLGLIAMSYGYVYVAKIAMGANDQQTLKALIEAESYDGPSLVIAYSHCIAHGYDM
                     AKGLEQQKLAVQSGHWPMYRYDPRLAMNGQNPLVIESKEPSIPFSQYAYNETRYKMLT
                     QMNEERAEELMKEAQHDAKSRWTLYQQMASMHYGNNNEEKKS"
     gene            complement(5555..5923)
                     /locus_tag="PL000_15005"
     CDS             complement(5555..5923)
                     /locus_tag="PL000_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90246.1"
                     /translation="MNSKPMMILIAGPYRSGTNDDPEKIAANVHFMESFALPIFRKGH
                     IPLLGEWFALPLVALAGSRQIGDEPFNEIFHPIAVMLLDKCDAVLRVGGASSGADQMV
                     SVAKEKGAQIFYSLDEIPQA"
     gene            complement(5924..6466)
                     /locus_tag="PL000_15010"
     CDS             complement(5924..6466)
                     /locus_tag="PL000_15010"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC90247.1"
                     /translation="MPFELIKSEPLLQGRAFKIRRDTMKTPDGRETKFDIVEHGGSVV
                     IIPVDGEGNLLFVRQYRHAAGMDMLELPAGTRDGDEPFAECAAREIREETGMEAGTLI
                     QVGAFYLAPGYSTEYMGVFLATDLKHNPLEADDDEFLSVEKIPVRDAIRSAERGELPD
                     AKTLAALLMAKPHLEKYLGA"
     gene            complement(6531..7286)
                     /locus_tag="PL000_15015"
     CDS             complement(6531..7286)
                     /locus_tag="PL000_15015"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNC90248.1"
                     /translation="MSNKQRGLLIMCVFVISNLLISACQMSASSAPAATPTALPTGQF
                     TSALPTGDNPMNMIAEFAKQTSVAQTTVANGGTPLAPQQTPATTGTVITAQPGTLPTA
                     TPTVGVPSTPTNAAAVATTPAVASTPSGPTTTPVPAGVRPATYTLQNGEFPYCIARRF
                     NVDPDELLSLSGLTSPDLYYPGLKLTIPQSGAFPGDRMLTSHPSTYTVASADENLYSI
                     ACKFGDVDPASLASANGISLSAALTIGQQIKVP"
     gene            complement(7352..9340)
                     /locus_tag="PL000_15020"
     CDS             complement(7352..9340)
                     /locus_tag="PL000_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90249.1"
                     /translation="MMNINQLKKFPALLFALITLVAYGLLVPFTGFYWDDWPFAFIAK
                     FLGPAEFNPAFAPFRPFLGPIFYFTTSLIPPVPLYWQIFALIIRFLIGITALWMFKQV
                     WPQRTNTALFAALLLLVFPGYSQHWVALTHINQELIPFIFYLLSFGFSFKGLHEQKTR
                     YIVIAILFQFCGIFPTEYFFGIEGIRFFLLFAFMQGSLLERFMKTIKAWWPFLLVWIL
                     NAVWLVYYYRFGPYTSYEVTATQSLTALSFLKEALDALWKAGIYIWFQILPLALTSLP
                     SPASILSLGLILLSFAFLALYLNPDTETTKTDPSFAISLILIGIPAILLGRLPSLAAG
                     LPLTLQSSYDRFMVSMMIGGSLFLLGMMELLFGKFRIKNYIFALVIALGIGQQFFNAN
                     IFRRDWENQRDIYWQMAWRIPALEPNTLLLTHQMPIDYETDISFTAPINWMYGTETSI
                     QNGLPYLMLYTEKRLGGTTLPSFAPDTPVTFPYRTRTFNGSTSQAIVFYMPQNGCLKV
                     FNPARGDLEIYSKLPDVLVDAIPLSDPSRIITDPATPATPMFFPEPEHGWCYYYTKAE
                     LAQQNNDHQTVMALADEAIALGYNPVDPYEWFVFIEAYAMQGDVQTAEKLSTNALASD
                     ARIRKSLCAVWEQVQVNGPEGSADEIQQVILTLKCNSQ"
     gene            9476..10579
                     /locus_tag="PL000_15025"
     CDS             9476..10579
                     /locus_tag="PL000_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90250.1"
                     /translation="MKKILHVLFLVCILMLQACGESPSPTAAVDVPEPTAIASAAAVV
                     TAGVVVPPTEATFIAAPPVTHVVIPSAGTSDRATAHDNENSLFFDTKKVKTGDEYFRN
                     RFERPFTAVDMNYLPDLDIVNFSINSDENFFYVKISMVGLDASTQTLNGSYGIEIDRN
                     ADGRAELLLTTRPPYSTEFTADNVAAYLDLNSNVGGGVINRPDSFGDDGFEAIIFDLN
                     KGIYPENDPDFLWVRQATDGELPAVEIAFARWMFKDNGKEAFMWSVISNANGLDPSKM
                     NYHDHYTPEQAGAANTDDPNYPLKELSALDNTCRVPLGFQASGTELLGCYMKSGDVEE
                     KATPVPDGPNSTCSIFAELCNRTKLTYQLYIPR"
     gene            complement(10615..11592)
                     /locus_tag="PL000_15030"
     CDS             complement(10615..11592)
                     /locus_tag="PL000_15030"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNC90251.1"
                     /translation="MREKILELLSGKQVNTQPVFSGLIHITAEGLQHEGLIFHEIHKD
                     AAKMAKAAASTFKLTGIPSAALPLDLCAPAEMMGAELNYYENMEYQFPQVKKALVESA
                     RDIKAVQFGAGRLELICEAISLVKNDIGGEAVISGLIPGPYTLLLYVCNPANMFMEMK
                     KEPQVVRDALTHLASFLAKVGQAYLNAGADFITIHEMGGSPGFIGPARFEQFILPALK
                     DLHANLPAPKVLSVCGKTDAAMHLLAQTGADAISVDQLNHLAASRSVLQDMLLFGNID
                     PVETLWQGDPAKVAESVVGAKEAGVNAIWPGCDLVPQTPLANLQSMKSA"
     gene            complement(11682..12563)
                     /locus_tag="PL000_15035"
     CDS             complement(11682..12563)
                     /locus_tag="PL000_15035"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNC90252.1"
                     /translation="MNKFLERLNSGEILVADGATGSNLQKMGLKPGKPPEDLIIDDPD
                     TILKLASSFAQAGSDIILTCTFGGTRMRMKDSKYQDRTPEVNIRAAEIARKAASLNGG
                     LVAGSMGPIGALVKPYGPVDADDVKATFAEQAKALADGGVDLLLIETMFSFEETTAAF
                     EGARSTTDLPIVISFSYDRGTRTMMGVKPKDVMKRYSEMGATLIGANCGTTLENMEAV
                     IKEYAATNSNIPLWVKPNAGVPHMDIETEQGVYDMGPEDMAAFSKKYVELGAKVVGGC
                     CGNTPEHIAAIVKAVKN"
     gene            complement(12659..14689)
                     /locus_tag="PL000_15040"
     CDS             complement(12659..14689)
                     /locus_tag="PL000_15040"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNC90253.1"
                     /translation="MNKHNIILQPSGRRGQVEEGMSVRSAARELGVEIESICAENATC
                     GKCMVLIEEGRFEKYNIDSKRDNVSPVTTEERAYLERRPKLLKDKGWEIGQVRLSCQA
                     KVCGDVLINVPEESRGNKQIVRKSASSREIEVKPAIRKYLVSMSPPTLERPIADWERL
                     AKGLETSMALVRGTEEKLPRWHELQIDYHCLKTLSPTLRAAKWNVTVSVWQDKEVIEV
                     QPGYVEDSYGAAVDIGSTTVALYLCNLRTGEMLAAESEMNPQIVYGEDVMSRIQYTIE
                     HKDGLEKLHKAIISTLNKLLKQAAKTAKIKTEEILEMVLVGNSTMHHILLNLHPKDLG
                     LAPFVPAIHKSMDIKARELGLHINSSGNIHVLPTIASFVGADTSAMIVAEEPHKQDEN
                     WLLIDVGTNAELVLGNRKRLVCTSTPTGPALEGAHVEYGMRAAPGAIERVHIDEKTLE
                     PKYKVIGVDGWNTEHAEFKGHVKGICGSAIIDSVAELFRAGIVDSRGKFKKGLNSNRI
                     REGESGWEYVIAWAEETSIGRDIPMTQQDVRQIQLAKAALFTAARTLLKRSGMQSPDK
                     IILAGGFGSYIDKEKAMLIGLIPDCDLENVYAVGNAAGDGARIALLNVEKRNEIDGVT
                     RKVDRFELPTDPEFQNNFMLATSFPHMSEPFEHIAHLIPNRVADPMAKNFIK"
     gene            complement(14748..15038)
                     /locus_tag="PL000_15045"
     CDS             complement(14748..15038)
                     /locus_tag="PL000_15045"
                     /inference="COORDINATES: protein motif:HMM:NF025142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HNC90254.1"
                     /translation="MSKYRVFYWKEIPASIIVEGDGRTIKKQLSQKVQNKIDAYAMVV
                     GLTSTEDYSAQYKRGAWVEREGAPEDVAEALLAELEAEAAKVEIPKREKTSE"
     gene            complement(15120..16160)
                     /locus_tag="PL000_15050"
     CDS             complement(15120..16160)
                     /locus_tag="PL000_15050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90255.1"
                     /translation="MKRLWVRLSLMISGVLFFMFFMQFLVITLEQSGMRSDLDGPPDA
                     PPAEIQGRLIEFMAFSIAVGLGGGVVIARVISTPINGLVNAAHQIGKGELSTRVPARG
                     SQEMIELSETFNKMAADLQHAEKLRNNLMADVSHELRTPLTVMEGNLRAVLDHVYPLD
                     ETVIANLYSQTHHLIRLVNDLRELSLAESGHLKLEKIPTDIQALIAETLQAIEPLSVE
                     KNIKMENEAVPTSDVTVDPFRIRQALFNLLSNALRHTPDGGTISIKTTSDDHAITISI
                     QDTGDGLTTDELASVFNRFYRADRSRSRETGGSGLGLAIVKAIIESHGGSVQAQSNGK
                     GTGSVFSICIPK"
     gene            complement(16160..16864)
                     /locus_tag="PL000_15055"
     CDS             complement(16160..16864)
                     /locus_tag="PL000_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90256.1"
                     /translation="MSQRILVVDDDRSIVKVICSYLEQSGYQTLAAYDGEMALHQIRR
                     ERPDLVILDLMMPNRDGWEVTQIVRADKALAPTPIIMLTARVEDTDKIVGLELGADDY
                     ITKPFNAREVVTRVKALLRRVKLDQSPHATPHILTCGGLKLDLDQRTLSVDAEPVELT
                     RTEFNLLEAFLSNPGYTLTRDDLLEKSMGYAYEGMGRALDTHIRNLRRKIEPDPDNPT
                     YIQTVYGVGYRLTGDK"
     gene            17006..17962
                     /locus_tag="PL000_15060"
     CDS             17006..17962
                     /locus_tag="PL000_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90257.1"
                     /translation="MKSNFVSSVSKIALAVLALVVVLVATGCSGLSVMPGGSASFASV
                     DPTYADLAYATQSDAQKLDLYIPEGSGPFPVVIMVHGGGFMFGDKADGAGLTGVDQLL
                     AAGYAVASINYRLSAEAQYPSQIHDAKASVRFLRANAEKYNLNPDKFAAWGASAGGNL
                     VSLLGTTCGVEELEGADLGNAEQSSCVQAVVDWFGPIDFLKMDEQFAGTSCEQSHNAA
                     NSPESKLVGAEIQTVPDLVATTNPMNYITADDAPFFIENGTADCNIPPIQNKNLADAL
                     SAVIGADNVTYVSLEGAGHGGSQFETAENLKLVIDFLDKNLK"
BASE COUNT         4248 a         4329 c         4742 g         4703 t
ORIGIN      
        1 gatggtactt ggagggcgac ctcctgggag agtaggtcat tgccaagggg cttttctttt
       61 taatatttat catgcataaa atcttctcat tcatgcatgt atcgtgtata atgcgaatgc
      121 agttttggca tggagttttt ctccatgaaa ttttgaggag tgtgaagcgc atcttttgat
      181 cttatggtca tctagttcgt ttcaggtctt gttataaagt cctcggctga ttctgcgtaa
      241 cggccgattt tttttggaca tttttattca gaaagtaggt gatttcattg gctacagtta
      301 atcttcgtaa cggcgaatcc caagattcat tgctcaagcg ttttcgtaag aaaatcgtca
      361 agagtggaat tctgagcacc attcgcaata agcgctggtt tgtctccaag agcgagaccc
      421 gtcgtatgga aaagaagaaa gcgattcgcc gtatcaagcg tcgctccttc aaggacactg
      481 aataaatcaa gaggcgtgta tgacaaaaga agaaggcaaa atcaaagttg atggtcttgt
      541 aattgaggca ttgcctggta ctcaattccg tgtgcggctt gacaatggtc atgaagtact
      601 ggcttatctt tctgggaaaa tgcgcaagca ttatattcgt attctccttg gagatcatgt
      661 ttccatggaa atgtcaccct atgatttgac ccgtgcccgc atcaccttcc gtcagcgcaa
      721 aaacacgcct gcaacacctt ctactactgg cagttaattt ccaaaaaaaa caagtcctcc
      781 atgaaaatgg aggacttgtt ttttttaaag tttttttctt ataccaaatc tctgaaagaa
      841 ttcagcacct tcatgtaatt ggcgcgttca aaagccgctg gttctttgac agatttctga
      901 ctcatgctgc cctgcatctg ttcaatggaa acatattcac gctctttcat ccacgcttgc
      961 aactcgtcta gcatggacgg aatgacctgt tcgccggttt gaagtaactt tgacgccatc
     1021 atggcaactt ttgcaccaga catcatggct ttgagtacat cgctgctagt gtgtacgccg
     1081 cttgtaagag caaaatctgc attgactttt ccatgcagaa ttgagaccca acgcaggggg
     1141 aggcgtaaat cagctgaagt gctaagatcc aggctgtgaa cgatttccag ctcattcagg
     1201 tcaaaatcag gctggtagaa ccgattgaat aatacaagac catcggcgcc agcttcaacg
     1261 atacggcggg catagtttgg cagggcggta atgaagggac ttaatttcac tgccaatgga
     1321 atggtgatcg ccgattttat ctcagccacc agttcgattt gggcgttttc caattcctga
     1381 gaggtcaatg ctgtgtcagt ggaaataaag taaaggttaa gctccagcgc atctgcaccg
     1441 gcatcctgaa tttgttttgc gtagtgggtc cagccgcctt ttgagacgcc gttcaagctg
     1501 ccgataacag ggatgttcag ggcttttttc aggccagtca gttgactcag gtacttctca
     1561 gggccgatgg agtacatgcc gctgtcaggc agataggaca gggcttcggc aaatgaatcg
     1621 gttccacgag tcaggtagtg gtccagttcg agcgattcgt ggatgatctg ctcttcgaat
     1681 agagagtaca tcacaattgc agaaatgcct gcatcctgca attttttagc cgactcgacc
     1741 ttttttgaga ggggagatgc tgaggctacc agcggattct ttaatttcag gccaagatag
     1801 gtcgtagaaa gatcggtcat gattttttct cctcattatt gtttccatag tgcatcgaag
     1861 ccatttgctg ataaagcgtc caacgggatt ttgcatcgtg ctgggcttcc ttcatcaatt
     1921 cttctgcacg ttcttcattc atttgcgtga gcatcttgta gcgggtttcg ttgtaggcat
     1981 actgcgagaa tggaatgctc ggttccttgg attcgatgac aagcggattc tggccgttca
     2041 ttgccagacg cggatcgtag cgatacatgg gccagtggcc tgattgcacc gcaagtttct
     2101 gttgctcgag gcctttggcc atgtcgtaac cgtgagcgat gcagtgactg tatgcgatga
     2161 caagggacgg cccgtcatag gattctgcct cgatcaatgc cttgagtgtc tgctggtcgt
     2221 tggctcccat ggcgattttt gcaacataca catacccata ggacattgca ataagcccca
     2281 gatccttctt tggcattccc ttgccgctca tggcaaattt ggcaactgct gcgcgtggag
     2341 tggatttgct ggattggccg ccagtgtttg aatacacttc cgtatccaaa accaacacat
     2401 tgacgttgcg cccgctggca agcacgtggt caagaccgcc gtaaccaata tcataggccc
     2461 agccatcacc accgatgatc cagaccgatt tctggaccag attgtctgca aggctgatca
     2521 ggtctttagc gcggctatcg cttgatttgc tgagtttgtt cttcaattcg gcgacccgtt
     2581 cacgttgggc gccaatgcct gcttcatctg cttgattggc gttcaaaatg gcttcaacca
     2641 actcggtgct tacagtcgtt tcgaacgatt tgagcagttc acgtgcatat tctttttgct
     2701 tatcgatcgt caggcgcata cccagaccga attcggcatt atcttcgaaa agtgaattgg
     2761 accaggcagg cccgcgccct tcggcattct tggcccatgg ggtggtgggg agattaccac
     2821 cataaatgga ggaacatccc gtggcatttg caatgatggc gcggtcaccg aacaattgtg
     2881 agacgagctt cacatacggg gtttctccac aaccagcaca ggcaccgcta aactcaaata
     2941 acgggcgcag caactgtgaa ttcttgatgg tggaggggtt gatcaacttg cgatcaaggt
     3001 cggggatgtt catgaagaaa tcccaattct tcgcttccgc ttcacgcaga ggtggttggg
     3061 cttccatatt aatggcgcgt cggccaaccg cacgtttatc ctttacaggg caaacttcca
     3121 cacacaaggt gcagcctgtg caatcttcgg gggctacctg aactgtatac gcatagccat
     3181 ttgagaattc cttgaacttc gaagcagtat gcttgaaggt ttcgggggca tctgccagca
     3241 ttttctcatc atatactttg tggcgaatga ccgcatgtgg acaaaccatc acgcacttgc
     3301 cgcactggat gcacagatct gcatcccata ctggaatctc aagcgccaga ttgcgttttt
     3361 cccactgcgt tgtgccggtg gggaaggtac cgtcaatggg gaacgcggaa acggggatat
     3421 tatcgccatc tgagtcgatg atctgcccaa gcacgttctt gacgaaatca ggcgcttcat
     3481 ccgggactgg gagtctgcgt gtcatctttg aggtgaccgc cgctggaact ttgacctcat
     3541 aaagattcgc cactgtggca tctacagctt caaagttttt ccgtacaacc gcttcgccgc
     3601 gcttgccata actcttttcg atggcatgtt tgatctcggc aattgccgca tcacgcggaa
     3661 gaatgccgct gatggcaaaa aaggccgtct gcatgatggt attcatacgt ccacccatgc
     3721 ctgttttttc agcgacatcg tacccgttga tgacgtggaa tttgagtttc ttggtaatga
     3781 tatccttctg cacttcctgt gggagttgat cccatacttc gttgggacca tacagactgt
     3841 tgagcaggaa gatgccaccc tctttggcat atttcagcat atccacacgc tcgaggaagg
     3901 aatattgatg gcaggcaacg aaatttgcct gattggcttc gatcagatac ggtgcgcgga
     3961 tgggtttggg cccaaagcgc aaatgagaca tggtcaccgt gccagatttc ttcgagtcat
     4021 agtagaagta accttgggca aagttttcag tctcttcccc gatgattttg atcgagtttt
     4081 tgttcgcacc cactgtcccg tcggagccga ggccgaaaaa cacacaacta accatgcctt
     4141 ctgattcaag tgagaaagag gggtccacac tcaaactggt gtttgaaacg tcgtcattga
     4201 taccgagggt aaaatgattc ttgggttcgg gtttggccaa ctcattcaaa accgattttg
     4261 tcatggcggg ggtgaattcc ttggacgaaa gaccataacg cccaccgatg actttgatat
     4321 tgctgcgatt accttccata agcgcattga cgatatcgag atacatcggt tcgccattgg
     4381 caccaggttc cttggtacgg tccagcacag caatggactt cacattcttg ggcaggccct
     4441 taaggaaatg ctcaatggag aatgggcgat ataaacgcac ccgcagtacc ccaacttttt
     4501 cgttcttttt tgcaaggtag tttacggttt cttctactac ttcaccgcca gaacccatga
     4561 tgataatgac acgttcggct tctgggtggc cgctgtaatc gaagaggttg taatggcggc
     4621 ctgtgatttt ggcgaattta tccatcattt cctgcacgat ggcaggtgtg gcggcataat
     4681 aattattcga agcttcccgc atttggaaat acacatctgg attttgcgca gttccgcgca
     4741 gggtgggatg ctcggggctg agtccacggg ctcgatgggc acggaccagg tcatcgtcca
     4801 ccatttgacg gagctcttca tccgttagtt gaaatatctt ggttacctcg tgtgaagttc
     4861 tgaacccatc aaagaaatgc atgaaaggga cacgcgattt tagagtggct gcctgcgcaa
     4921 tggctgcaaa atcctgagct tcctgaaccg agccagagga gagcaaagcc cagcccgtct
     4981 gacggacagc catcacatct tgatggtcgc cataaatgga aagcgcatgg gatgcaatgg
     5041 cacgtgcggc cacatggaag acggtgctgg tcaattcgcc cgcgatcttg tacatatttg
     5101 ggatcattaa caataagccc tgagatgcgg tgaaggtggt tgtcagtgca cctgtttgaa
     5161 gcgccccatg tacggtccca gctgcgccgc cttcagattg catttcaatg acgagtgggg
     5221 tggttcccca aaggtttttc tttcctttgg ctgaccattc atcagaaaat tcacccatgc
     5281 cagaggatgg ggtgatcggg taaatggcga tgacttcgct tagacggtgg gcaacgcttg
     5341 cggtcgcctc attaccgtcg atcataatga tgggttgttt cattcttgct cctttgtaag
     5401 caggcttaca cctgggaatt tcttcaattt acaagtgtat cccccttaca actctaaggt
     5461 tagttattat ccgcttaaat atatgtgctt gttgtcacat tttggacacg aatggtggaa
     5521 ataaaaagcg gcggaatatc cgccgctttg ttgattatgc ctgaggtatt tcatcaagcg
     5581 aataaaatat ctgtgcccct ttttccttcg ccacacttac catttgatct gccccgctcg
     5641 aggccccacc cacacgaagg acggcatcgc atttatcaag aagcattaca gcaatgggat
     5701 gaaagatttc attgaaaggt tcatcgccga tctgcctgga ccccgccaga gcaaccagtg
     5761 gaagcgcaaa ccattctccc aataggggaa tgtgcccctt gcggaaaatc ggtagcgcaa
     5821 acgactccat aaaatggaca ttggctgcga tcttttcagg atcatcattg gttcctgagc
     5881 ggtatggccc tgcaatcaga atcatcatgg gcttgctgtt catttatgct cctaaatatt
     5941 tttcaaggtg aggtttcgcc attaataagg ccgccaatgt tttggcatct ggaagttcgc
     6001 ctcgttctgc gctgcgaatt gcatctcgaa cagggatctt ctccacggat agaaattcat
     6061 catcatctgc ttcaagcgga ttatgtttca aatcggtggc caggaagacc cccatgtatt
     6121 cagtggaata tcccggtgca agataaaacg cgccgacttg aatcagtgtg ccagcctcca
     6181 tgcctgtttc ttcgcggatc tcacgcgcgg cacattcggc aaagggttca tcaccatcgc
     6241 gtgtgccagc agggagttca agcatatcca tccccgccgc atggcgatat tggcggacga
     6301 aaagcaggtt accttccccg tccacgggga tgatcaccac tgaaccgccg tgttcaacaa
     6361 tgtcgaattt ggtttcgcgg ccgtctgggg ttttcattgt gtcgcggcga atcttgaagg
     6421 cacgtccctg taacaagggt tcagatttga tgagttcaaa aggcatatgg gtctccttgg
     6481 tagcaaaaaa gcccaagcat gcgcctgggc ttttttatca gcatgatcga ttacgggact
     6541 ttgatctgtt gaccaatggt caaagcggcc gagagggaaa ttccattggc agaggcgagc
     6601 gaggcgggat caacatcgcc gaacttgcag gcaatgctgt acagattttc atctgcagag
     6661 gccacggtat aggtggaagg atgcgaagtc aacatgcggt caccggggaa cgcgccgctc
     6721 tgcgggatgg tcagcttaag accggggtag tacaggtcgg ggctggtaag tccgctcagg
     6781 ctgagcaatt catcggggtc tacattgaaa cgacgggcaa tacaatatgg gaactcacca
     6841 ttctgcaggg tataggttgc agggcgcacc ccggcaggga cgggggtggt ggtggggccg
     6901 cttggggtag aggcaacggc aggagtggtg gcaacagcag ctgcattggt gggagtgctc
     6961 ggtacaccaa cagtgggagt tgcggtgggc agggttcccg gctgggcagt gataacagtg
     7021 cccgtagttg caggggtttg ctgcggggca aggggagtgc cgccgtttgc cacagtagtt
     7081 tgagccactg aagtttgttt tgcaaattca gcgatcatgt tcatggggtt gtcaccggtg
     7141 ggcagggcag aggtgaattg tccggtgggc agtgcagtgg gggttgcggc cggtgcggaa
     7201 gatgccgaca tctgacaggc agagatcagc aagttcgata ttacaaagac acacatgatt
     7261 agcaagccgc gttgtttatt actcatagtt tctcctgttt cgatatactc agtgggcgat
     7321 ggtagcacat gaactttaaa gcgtcaagcg attattgcga attgcactta agtgtcaata
     7381 ttacctgttg aatctcatct gcgcttcctt ctggaccgtt gacctgcacc tgttcccaga
     7441 cagcgcacaa gcttttgcga atgcgtgcat ctgatgcaag cgcgttagtg gatagttttt
     7501 ctgcagtctg aacatctccc tgcattgcat aggcttcaat gaagacgaac cattcataag
     7561 gatcaacggg gttatagccc agcgcaatgg cttcgtccgc cagtgccatc acggtttgat
     7621 gatcattatt ttgttgagct aattcggctt tggtgtagta gtaacaccag ccgtgttcag
     7681 gctcggggaa gaacatgggt gttgcaggtg tggctgggtc agtgatgatg cgcgatggat
     7741 ctgaaagcgg aatggcatcc acaagaacat cagggagttt tgagtagatc tccaaatctc
     7801 cacgagcggg gttgaacact ttcaggcatc cattttgagg catgtaaaag acgatggctt
     7861 gtgaagtgga cccattgaat gtgcgggtgc ggtaagggaa tgtgaccggg gtatctggtg
     7921 caaaagatgg cagggtggtg ccgccaaggc gcttttcagt gtagagcatc aggtagggaa
     7981 ggccgttttg aatggatgtc tctgtgccgt acatccagtt gatcggtgcg gtgaaggaaa
     8041 tgtcggtctc gtagtctatg ggcatttggt gggtgaggag cagggtattc ggctccaggg
     8101 cagggatgcg ccatgccatt tgccagtaga tatcgcgctg gttttcccaa tcgcggcgga
     8161 agatattggc gttgaagaat tgctgtccga ttccgagcgc gatgaccagg gcgaagatgt
     8221 aattttttat gcggaatttc ccaaaaagga gttccatcat acccagaagg aacaaactgc
     8281 caccgatcat catagaaacc atgaagcggt cataagagga ttgcagggtg agcgggagtc
     8341 ctgcggccaa ggatggaagc ctgcccagca aaatggcggg aataccgatg agaatgagcg
     8401 aaatggcaaa agacggatcg gtttttgtgg tttctgtgtc ggggtttaga taaagggcga
     8461 gaaatgcaaa agaaagcaga atcaggccga ggcttaagat ggaagcgggc gatgggagtg
     8521 aggtcaatgc caggggcaga atctggaacc agatgtaaat tccagctttc cagagagcat
     8581 ccaacgcttc ctttaaaaag gaaagtgcgg tcagtgattg tgtggctgtg acttcgtaag
     8641 aggtgtatgg gccgaatctg taataatata cgagccacac ggcattgaga atccagacga
     8701 ggaggaaggg ccaccacgct ttgattgttt tcatgaagcg ctcaagcaag ctgccttgca
     8761 tgaatgcgaa aagcaaaaag aaacgaatgc cttcgatgcc gaaaaaatac tcggtgggaa
     8821 agatgccgca gaattgaaaa aggattgcga tgacgatgta tcgggttttt tgttcgtgca
     8881 gccccttgaa gctgaagccg aaggagagca ggtaaaaaat aaaaggaata agttcctggt
     8941 tgatgtgggt gagtgccacc cagtgctggc tgtaacccgg gaagacgagg aggagcaatg
     9001 cggcaaacag agcggtgttg gtgcgttgcg gccatacctg tttgaacatc cacaaggcgg
     9061 tgatgccgat cagaaagcgg ataatgagtg caaatatctg ccagtaaagc ggtacaggcg
     9121 gaatcaggct ggtggtgaaa taaaagatcg gcccaaggaa tgggcggaag ggagcaaaag
     9181 cagggttgaa ttcggctggg ccgaggaatt tggcgatgaa ggcgaagggc cagtcatccc
     9241 aataaaaccc ggtaaaggga accagcaagc cataggcgac aagcgtgatc aaagcgaaca
     9301 acaaagcagg gaattttttt agttgattta tattcatcat gtttttgtaa ttataagata
     9361 gcagaccttt cgtttttgtt aggtataatg attttattct aaacctattt cctacaagat
     9421 gaaattatag ttaatgatat attgatgccc tgcatctcaa aacgaggaaa tgaagatgaa
     9481 aaaaatactt cacgtattat ttttggtttg tatattaatg ctgcaggcgt gtggggaatc
     9541 gccttctcca actgccgcag tggatgtccc tgaacccacc gccattgcaa gcgcagcggc
     9601 ggttgttact gctggtgttg tcgtgcctcc aaccgaggca acttttatag ctgccccgcc
     9661 tgtgacccat gtagtcattc catcggcagg gacgagtgat cgtgccaccg ctcatgataa
     9721 cgagaattcc ctgttctttg acaccaaaaa ggtcaagaca ggagatgagt attttagaaa
     9781 tcgcttcgaa cgtccattta ccgctgtgga tatgaattac ctgccagacc ttgatatcgt
     9841 gaatttctcg atcaatagtg atgagaactt cttttatgtg aaaatttcaa tggtggggct
     9901 cgatgcttcc acccaaacgc tgaacggctc ctacgggatc gaaatcgacc gtaatgccga
     9961 tggccgcgct gaattgttat tgaccacccg cccgccctat tcaactgaat ttactgccga
    10021 taatgtagcc gcgtatctcg atctcaacag caatgttggc ggcggcgtga tcaaccgccc
    10081 tgattcattc ggcgatgacg gatttgaggc catcattttc gacctaaaca aagggatcta
    10141 cccggaaaac gaccctgatt ttctctgggt gcgccaggct acagatggcg aattgccagc
    10201 agtggaaatt gcctttgcac gctggatgtt taaagacaat gggaaggaag ccttcatgtg
    10261 gagtgtgatt tcaaacgcca acgggcttga cccatccaaa atgaattatc acgatcacta
    10321 cactcctgag caggcaggcg cagccaatac cgatgacccc aattatcccc tcaaggaact
    10381 ttcggcactg gataatacct gccgagtgcc tctcggtttt caggcttcag gcactgaact
    10441 tttaggctgt tacatgaaga gcggcgatgt ggaggaaaaa gccacacccg tgcccgatgg
    10501 ccccaactcc acctgtagta tttttgcaga gttgtgtaac cgtacgaaac ttacttatca
    10561 actttacatt ccacgctaaa ccaagatgat aaaaaaagcg cctgacgaaa tccgtcaggc
    10621 gcttttcatt gattggagat ttgccaacgg tgtttgcggg acaaggtcac aacctggcca
    10681 gatggcattc actcccgcct ctttcgcacc gacaactgac tcagcaactt tggcaggatc
    10741 tccctgccag agagtctcaa cagggtcaat attgccaaac aaaagcatat cctgcaaaac
    10801 ggaacgcgat gcggcaagat gattcaactg atcgaccgaa atggcatccg cacctgtttg
    10861 ggccagtaaa tgcatggcgg catccgtttt tccacatacg gataatacct tcggcgcagg
    10921 aaggtttgca tgcaggtctt tcagtgcagg caggatgaat tgctcaaacc tggcagggcc
    10981 aatgaaaccc ggtgacccgc ccatctcgtg aatggtgata aagtcggcgc ctgcattgag
    11041 gtaggcctgc ccaacttttg caaggaagga agcaagatgg gtgagggcat cccgtacgac
    11101 ctgcggctct tttttcattt ccatgaacat gttggcagga ttgcatacgt acagcagcag
    11161 ggtatatggc ccagggatca aacctgagat gaccgcctct ccgccaatat cattctttac
    11221 cagactgatc gcctcacaaa tgagctctaa ccgcccagca ccaaattgaa ccgccttgat
    11281 atctctggct gattcaacca atgctttctt cacctgcggg aactgatact ccatgttttc
    11341 atagtaattc aattcagcac ccatcatttc agcgggcgca cacagatcca acggcaaagc
    11401 ggcggaagga atcccggtca gcttgaatgt gctggcggcg gcctttgcca tttttgccgc
    11461 gtctttgtga atttcgtgga agatcagccc ttcatgctgc aacccctcag cggtgatgtg
    11521 gatcagcccg ctaaaaacag gctgggtatt gacttgtttc cctgacaaca actctaagat
    11581 tttttcacgc atggttgcat ccttttacaa aatacggatt atgaattccg tgtaccgcaa
    11641 ctcataatcc gtacctgatt cctgtctatt ttttgttgtt actaattctt tacagccttc
    11701 acgattgcag cgatatgttc tggggtattc ccacaacaac cgccgaccac tttggctccc
    11761 agctcaacat atttctttga gaaggcggcc atgtcttcag gacccatatc atacacacct
    11821 tgttcggtct caatatccat gtgtggaacg cctgcattgg gtttgaccca cagagggata
    11881 tttgaattgg tggccgcata ttccttgatc accgcttcca tgttttccaa cgtggttcca
    11941 cagttggcgc cgatgagggt tgctcccatt tcggaatagc gcttcattac gtctttgggt
    12001 tttacgccca tcatggtgcg tgtgccgcgg tcgtagctga atgagatcac aatggggagg
    12061 tctgttgtag agcgtgcccc ttcgaaggca gcagtggtct cttcaaaaga gaacatcgtt
    12121 tcgatcaata acaggtccac accgccatct gcgagggcct tggcttgttc ggcaaaggtt
    12181 gcttttacat cgtccgcatc cacggggccg tagggtttga ccaaagcgcc gatcgggccc
    12241 attgaaccag cgaccagccc tccgttaaga gaggccgcct tgcgggcgat ctctgctgcg
    12301 cggatgttca cttccggagt gcggtcctga tatttcgaat ctttcatccg catgcgtgtg
    12361 ccgccaaagg tgcaggtcaa gataatatcc gagcctgcct gtgcgaagga agaagcaagt
    12421 ttgagaatcg tgtcagggtc atcaatgatc agatcctcgg gtggcttgcc aggcttgagg
    12481 cccatctttt gtagattcga acctgttgct ccatccgcaa caagtatttc cccgctgttc
    12541 aatcgttcca aaaacttatt catacctgct ctccttgttt ccacgaaggg acacaaaggt
    12601 cacaaaggta ttagatcttt tcctttgtga cccttcgtct ccttggtggt gaaaaaaatt
    12661 attttatgaa atttttcgcc atcgggtccg ccacacgatt ggggatcaaa tgtgcaatgt
    12721 gttcgaacgg ctcgctcatg tgcgggaagc ttgtcgcaag catgaaatta ttttggaatt
    12781 caggatcggt gggcagttca aagcgatcta ctttgcgggt tacaccatcg atctcgttgc
    12841 gcttttcaac attcaacaat gcaatgcgtg cgccatcgcc agcggcattt ccaactgcat
    12901 acacattttc caggtcgcaa tcggggatga gcccgatcag catggctttc tctttgtcaa
    12961 tgtaacttcc aaagccgcca gcgaggatta ttttatcagg agattgcatc ccgctgcgtt
    13021 ttaataatgt ccgtgccgcg gtgaacaaag ctgcctttgc cagttgaatc tggcgcacat
    13081 cctgctgggt catggggatg tcgcggccaa tggaagtttc ctcagcccag gcgatcacat
    13141 actcccagcc gctttctccc tctcggatcc gattcgaatt taaccctttc ttgaacttgc
    13201 cgcgagaatc aacaatgcct gcccggaaca attctgccac cgagtcgatg atcgctgagc
    13261 cgcagattcc cttcacgtgc cccttgaact cggcatgttc cgtgttccaa ccatccacgc
    13321 cgatgacttt gtattttggt tcaagtgtct tctcgtcaat atggacgcgc tcaattgcgc
    13381 caggcgcagc ccgcattcca tactccacat gagcaccttc aagtgcaggg cctgtggggg
    13441 tggaggtgca gaccagccgc ttgcgatttc caagcaccaa ttcggcgttg gtgccgacgt
    13501 cgatcaacag ccagttttca tcctgtttgt gcggttcctc tgccacgatc atggcgctgg
    13561 tatcggcgcc gacaaaggag gcaatggtgg gcagcacatg gatattgccc gatgaattga
    13621 tgtgcagacc caactcacgc gccttgatgt ccatggattt gtgaatggca gggacaaatg
    13681 gcgccagccc caaatccttc gggtgcaggt tcaacaaaat atggtgcatg gtggaatttc
    13741 ccaccagaac catttccaga atttcctcgg ttttgatttt tgcggttttg gctgcctgct
    13801 ttaataactt gttcagagtg gagatgatgg ccttgtgtag tttttccagg ccatccttgt
    13861 gttcgatggt gtattgaatg cgcgacatca catcttcgcc gtacacgatc tgcggattca
    13921 tttcggattc agcagcgagc atttcgcctg tgcgcaaatt gcacaggtac aaggccaccg
    13981 ttgttgagcc gatatccaca gcggcgccgt agctgtcttc cacatatcca ggctgcactt
    14041 cgatcacttc cttatcctgc cagaccgaaa ccgtgacatt ccattttgcg gcgcgcaggg
    14101 tgggggagag ggttttcagg cagtggtaat caatttgcaa ttcgtgccag cgggggagtt
    14161 tttcttctgt gccgcgaacc agtgccatgg aggtttcgag gcccttggcg agacgctccc
    14221 aatcggctat ggggcgctcc agcgtgggcg ggctcatgga aaccagatat ttacggatcg
    14281 caggtttgac ctcgatctcg cggctgcttg cgctcttgcg gacgatctgc ttatttccgc
    14341 ggctttcttc aggcacgttg atcagcacat cgccgcagac cttggcctga catgaaagcc
    14401 tcacctgacc gatctcccag cccttgtctt tcaatagttt gggtctgcgc tcaagatatg
    14461 cacgttcctc cgtagtaaca ggggatacgt tatcacgctt cgaatctatg ttgtattttt
    14521 caaagcggcc ttcttcgatc aagaccatgc actttccgca ggtggcattt tcggcacaga
    14581 tcgattcgat ctccacaccc agttcacggg ctgccgaacg gacagacatg ccttcttcga
    14641 cctgaccacg gcggccagag ggttgcaaaa tgatgttgtg tttgttcata atattgacaa
    14701 tagacggcgg acgaccgacc acgatcagcg gctatcgtcc acggtcatta ctccgaagtt
    14761 ttttcccgct tgggaatttc tacctttgct gcttctgctt ccagttccgc cagaagtgct
    14821 tcggccacat cttcaggtgc gccttcgcgt tcgacccagg ccccgcgctt gtattgcgcg
    14881 ctgtaatctt cagtggaggt taacccaacc accatggcat aggcatcgat cttgttctgc
    14941 accttttgtg agagttgctt tttgatggtg cggccatctc cttctacgat gatggaagca
    15001 ggaatttcct tccagtaaaa aacgcgatat ttgctcatgt cagttccttt aaattatata
    15061 taatttgtgt cagcgatttt acgtgaggtt ggcatagcgg tcaatgtaaa aaataaaaat
    15121 tattttggaa tgcagattga aaagacgctg cctgtgccct ttccattact ttgtgcctgc
    15181 acactgccgc catgcgattc gatgatcgct ttgacaatgg ccagccccaa tccgctgccg
    15241 cctgtttcgc gtgagcgcga tctatcggcg cgataaaagc gattgaaaac agaagcaagc
    15301 tcatcggtcg tcagaccgtc gcctgtgtcc tgaatcgaaa tcgtgatcgc atgatcatcg
    15361 cttgttgttt tgatcgaaat cgtcccgcca tcaggggtgt gccgcaaggc gttggatagc
    15421 agattaaaca gcgcctgccg aattcgaaat ggatccacgg tcacatcaga cgttggaact
    15481 gcttcgttct ccattttgat atttttttca actgagagag gttcgatcgc ctgaagtgtt
    15541 tcagcgatga gtgcctggat atcggtgggg attttttcca acttcaaatg acccgactcg
    15601 gcgagtgaca actcgcgcag atcgttgacc agccgaatca gatgatgggt ttgactgtaa
    15661 agattcgcga tcaccgtttc atcgagcgga tacacatgat cgagcacggc gcgcaaattg
    15721 ccttccatca cggtgagcgg tgtgcgtagt tcgtgcgaga catcagccat taagttgttg
    15781 cgcaattttt ctgcatgttg cagatcagcc gccattttgt tgaaggtttc tgaaagctcg
    15841 atcatttcct ggctgccgcg ggcgggtacg cgtgtggaga gttcgccctt gccgatctga
    15901 tgcgcggcat tcacaagccc gttgatcggc gtgctgatca cacgcgcaat gaccacacct
    15961 cctccaagcc cgactgcaat tgaaaaagcc atgaactcga tcaggcggcc ttgaatttcg
    16021 gcgggcggcg catcaggcgg gccgtcgagg tcggatcgca ttccactctg ttccaatgtg
    16081 atcaccagga actgcatgaa gaacataaaa aacaacaccc cgctgatcat caaactgagg
    16141 cgcacccata atcgcttcat tatttatctc ctgtcaaacg atagccaacg ccatagacag
    16201 tttgaatgta ggtcgggttg tctgggtcgg gctcgatctt gcggcgcaaa ttgcggatgt
    16261 gtgtgtcgag tgcgcgtccc atgccttcgt aggcgtagcc cattgatttc tcgagcaggt
    16321 catcgcgggt gagtgtgtaa ccggggttgg atagaaatgc ttcgagtaaa ttgaactcgg
    16381 tgcgggtgag ttcgacgggt tctgcatcca cacttaatgt gcgctgatcg agatccaatt
    16441 tcaggccgcc gcaggtgagg atgtgaggtg tggcgtgtgg agattgatcc aatttgacgc
    16501 ggcgcagcag ggctttgacg cgtgtgacca cttcgcgggc gttgaacggc ttggtgatgt
    16561 agtcgtcggc gcccaattcc agcccgacga tcttgtctgt atcttccacg cgggcggtga
    16621 gcatgatgat gggggtgggc gcaagcgctt tatctgcgcg gacaatttgg gtgacttccc
    16681 aaccgtcacg gtttggcatc atcaggtcga ggatcaccag atcggggcgt tcgcggcgga
    16741 tctggtgcag cgccatttcg ccatcgtagg cggcaagggt ttgataacca gattgttcca
    16801 ggtatgagca gatcactttt acgatggaac ggtcatcatc tacaacaaga attctttgtg
    16861 acattttcta cctcgtatcg agtcatcata gcatatcatt ctcaaggaaa tctcaagaat
    16921 acaaacaggt catcttcagg atttcttgac aagtctttgt gatactgggg gagttcgatc
    16981 aatcattttg tagatggaga caccaatgaa atctaatttt gtaagttctg tttcgaagat
    17041 cgcgctggct gtgctggcgc tggttgttgt gctcgttgcc acggggtgca gtgggttgtc
    17101 ggtgatgccg ggcggttctg ctagttttgc ttccgttgac ccaacttatg cagacctcgc
    17161 ttatgcaacc cagtctgatg cgcagaagct ggatctgtat attcccgaag gcagtggacc
    17221 tttcccagtt gtcattatgg ttcatggcgg cgggtttatg ttcggtgata aagccgatgg
    17281 cgcggggctg acgggtgtgg atcaacttct cgctgctggc tatgctgtgg cgagtatcaa
    17341 ctatcggtta agtgcagagg cgcagtatcc ttcgcagatt catgatgcaa aggcatctgt
    17401 ccgctttttg cgcgcgaatg cagagaagta caacctcaac cccgataagt ttgcggcgtg
    17461 gggcgcttct gcgggcggga atttggtgtc gctgcttggc acgacctgcg gtgtggaaga
    17521 attggaaggc gcagacctcg ggaatgcgga gcaatcgagt tgtgtgcagg cggtggtgga
    17581 ttggtttggc ccgatcgact tcttgaaaat ggacgaacag tttgcgggga caagctgcga
    17641 acagagtcat aatgccgcca attctcccga atcaaaactg gtcggcgcgg aaattcaaac
    17701 cgtgcctgat ctggttgcaa ccaccaaccc gatgaattac atcactgcgg acgatgcgcc
    17761 tttcttcatt gaaaacggca cagccgattg caatattccg cccattcaaa acaagaatct
    17821 tgcagatgcg ctcagcgcgg tgatcggcgc ggataacgtg acctacgttt cactcgaagg
    17881 cgcggggcat ggcggctctc agtttgagac agccgagaac ttgaaactgg tgattgactt
    17941 tttggataag aatttgaagt agttttttgg gagatgtaaa aagatgagcc gctcaggctc
    18001 atctttttgc ttgtgtgaat tg
//
LOCUS       DAOQLC010000092        17600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1135_length_17600_cov_21.153149, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000092 DAOQLC010000000
VERSION     DAOQLC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_17600_cov_21.153149"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1452)
                     /locus_tag="PL000_15065"
     CDS             complement(139..1452)
                     /locus_tag="PL000_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90258.1"
                     /translation="MRIVNGRVALVFALAALFLLTGCGGGEKYTYRVNGLAGQAHVEY
                     ASPDGSMVEEDVQLPWSIEVDLGNSAEFTLYATNASEFGTVHCEVLKGEFLVGDASGS
                     VFAGCSGSVTGSGDNTKVNFKGFDDVPPDSRSLPTSGGLVAGNELIMAVGGNVLDGLD
                     LYVLDPASPGRMAKLTKGLYSAFCPAISPDGTKIAFRKRDIRWDIFVMTVSDSTLRRI
                     TDNEAEEGCPVWSPDGQNLAFDSRNESAFSEIILSSSDGRTQNLLTAALRAEQPEANF
                     YSPNWSPDGKQIAFTLRLKDNDIYLMNIEDGSASPLEVTPDQNEAFPRWSPDGESVLF
                     FRKDGIYIGKADGSGSVKVNTGSLIPDNANWSPDGARIVFSADDKETEIKTKALYLVN
                     LDGSGLTLFAEIPNTDLFDPFWQSFASDSPMLKLQSLPVVPTTQQ"
     gene            1589..2029
                     /locus_tag="PL000_15070"
     CDS             1589..2029
                     /locus_tag="PL000_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90259.1"
                     /translation="MNTKNNLNFWWRWLVTANIGVILFGLTQALLPNLVRQGFSLMVF
                     GSASYIDSFPLDAIRYITLTHAVMGAVMIGWGVSMMYTLFTQFRKGEWHGWLSLVVAL
                     ILWFIPDTAMSIATGFWQNAVLNTVFAILFVIPLSATYQIFRRA"
     gene            2033..2413
                     /locus_tag="PL000_15075"
     CDS             2033..2413
                     /locus_tag="PL000_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90260.1"
                     /translation="MNNQLNLSRILLANGVFCSTSGLIFLLAAKPLSEFLNTVPVVMS
                     VLGIGLIAYGVWVIYMATRRLITNFFSIFVIGADLLWVLFSVLLLILPAFNFSAEAKW
                     AIGITAICVDLFATVQFLEWRKMR"
     gene            2547..2918
                     /locus_tag="PL000_15080"
     CDS             2547..2918
                     /locus_tag="PL000_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90261.1"
                     /translation="MAKILVVEDIQDNFELVRRALSGTDHQIIHAPDAETGLQSALDT
                     IPDLILLDLGLPDHDGQTLAGWLRETPELANTPIVALSAWPEENAKEMVESYGFSGYI
                     SKPITRVADFIARINSFLKAQ"
     gene            2919..3794
                     /locus_tag="PL000_15085"
     CDS             2919..3794
                     /locus_tag="PL000_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90262.1"
                     /translation="MQTPDSNEPIQLIPASRFNYEELTAIYNQTRVDYMIPMPMNAAR
                     LAEYVRIYDVDLDHSLVAATHDGQMLGVAMLGIRKKRVWLTRLGVLPNTRRSGIGSAL
                     VGGLIEDAAKLGLDFVMLEVIKNNTPAHQLFLKYGFREIGELLVLRRSPLIPPIDPIV
                     ADAQRMDRTEALVLVGHDRGTQPWTNQSESLINAHEVSGLSITLVDGSRGWLVYQREK
                     FLLTRFAIKTEAGDPATVAYALLTHLHSQYPRLDTHIENIQVNDPHLPAYYKMGYVES
                     FRRIEMWRGDFPSSN"
     gene            complement(3800..5563)
                     /locus_tag="PL000_15090"
     CDS             complement(3800..5563)
                     /locus_tag="PL000_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90263.1"
                     /translation="MANQKWCVINKATLCESPKGATVTEIYLGALVEVTGRQEDVPML
                     GKDGQTYTSTWVECVYRNSQGTVAGWVRDEFFDDYVEKFPPPVVEIPAATEEQMGEFP
                     YATKNPNDPAQYLVLGRNSKGEEQVKYNLCAEFCVAFVMGVGIKQFLRDWENSAGSLY
                     KWALGGDSDKTSDLSLVKNMLNPYSYSAANGNIIDFAVTLIGPAFEDKTLSPGRFQKM
                     LQTHSLLAQVVINKNTGKLIPNNETKRANGGINHWIVLESATPNGTDGGRVEIYNPFN
                     NQRQEYSYEYFISSFGGGTYTGCWVKRRSEMVAVKAMDEVASAKKWCIAPGTFRQTPD
                     GVGVLSFMKGAVMESSGATEVKNGKTWIQVKYKSRTGWVRNSVLEDYEDRFSTPEVII
                     PHPTPEEDDAEQYMFLPGEDGKKRNMCGQLCASFIMNIDVESFVQDWKLKARPYYQVS
                     IAGKNDNGTGIDSLETMFRASPYNAAPGDVFRFELGMKDPVTGILILSAGRMKKMLET
                     HYLVAGVRIRQSDKLTGRLSGQGIGHWVVVDKVFPNGKNSGNGGWVEIYNPYPNRRQE
                     YAYDEFMQSFAGTLGLWVRRK"
     gene            5825..6565
                     /locus_tag="PL000_15095"
     CDS             5825..6565
                     /locus_tag="PL000_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC90264.1"
                     /translation="MRIAFISDIHGNFTALQAVLDDIKVQNIDEVICLGDTVSLGPQP
                     REVLDRLRELNCVIIKGNHDSAILDPEEAEKFEITSHLIPDLYWCRERLTKNDLDFIA
                     SFKTLHEISMPNGVKILCFHGSPLSPTDIIQATTPPETLDKYFEGHQATVFIGGHSHI
                     QMYRRHGDKLILNSGSVGNSFMFAFTPGNAPRLLPWAEYTIISQNGNSLDVDLRRVYF
                     DTTVLMEAVQASGIPSAEWWFRQYATTK"
     gene            6756..8135
                     /locus_tag="PL000_15100"
     CDS             6756..8135
                     /locus_tag="PL000_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90265.1"
                     /translation="MFVTKNLYQNDETWKSTPLGNSSETIGGWGCLLTSVTMMLNGIG
                     YNETPVTANEKFKAAGGFSGALFMPSYLPYVWPNCAYRDMQPCEAYPAPIPQIDAAVA
                     AGKPVILQVDWNKQAGIQTHFVLVKEKKGDDYVLYDPYKYSGDGPDKEVLLTTRYKYN
                     GAKLTSEISAVLWFDSYSSTPPEPPKVTKVPVPAEKYLLYAGEDDLALRSEPSVSGYL
                     WKRMLLGTELICLEDKAKAKSKIGVQGQWIQVQDPKGDQGYVAAWFVSEQKGKPAAST
                     TTTSTPAAAPAPVAAAPAPVPAGALALYPTEELSFRSQPVISPETLVRRVPVTEQLIC
                     LEAANTAIPKVGVQNQWLKVRGADKKEGYVAAWYVKYAGGSTAQAAATSAPAATGSKK
                     VKTTVEMVSLRNQPVVSDATLIKRVPINYEFTITEAGGEAKVGKNDQWIKVKDATNEG
                     YVAAWFVSF"
     gene            8154..11186
                     /locus_tag="PL000_15105"
     CDS             8154..11186
                     /locus_tag="PL000_15105"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC90266.1"
                     /translation="MEESSRTTKIFISYSRKDKLFVRKLNAALDAAGIDAWVDWEGIE
                     LASDWMARITAAIEGGDAFVFVISPDSLKSKVCMDELELGISSNKKIIPVLYREPEKK
                     QKMHPKLASTNWVYLRPKKDDFKNTIPKLIEAIQTDLEWVQQHTRLLQRATEWDQKRR
                     NKSYLLQGTDLEDGEQWMTASTTGPNRSVNPMQAEYISTSRKTSVQRQRNLTIGVGAM
                     LVISIFFGIFAFLQRNIARQSERDAVNSRSTAVANELARATQQAIAEEQRVIAEQQKL
                     IAQENEEKAEAQRSAAEAKIYQDRDGELDTSTLLAVNALQKLEGLDDAEDILRNNITL
                     LPIPVKQMNLGARVWAIQPSADQSKFVTTDSAGKACVWSMEDGSQFFCAQHDGIVYDA
                     NFSQDGTILATGTEKGVLTFWNVDTGEQIKSIQYEGTIWDLNPHPGGRWLGVGRSNAI
                     SMIDMTDLTEALFITLSGEVKTIDFDDTGSFMAMGTSKGNVTVWAVMGNQSVGGPKHN
                     GEVIDVELSPDGNWVVSVGQDSTARSAQSRAGGQKYSVTHGDWVEDVTFGPDSSWFVT
                     ASDDNTVRVIDTATGQERLRMVHNNFVQKVRVSGDGQWIATTGYDKTLRIWDAASGAE
                     TMQIPLEGIGSSIRFNRDATRLIVGDYDGHVTLWNISELKARRGFQQFSEFVNEAHFS
                     PDGKWLAVNTDDRKIWRIDLDESGNPQGERKVIAETDGLTSDMAISADSNWVAAVEND
                     ENIADYNRVVLARADGSNEFFLSHDTQVITSTAFTPDSKQIITADEKGVIHIWNVETG
                     EKVNSLSADGIILTLAVSPNGKYLVAGIEDANHSLVWDLTTMTQVKVLEQIGKIKSVQ
                     FSNNGNLLATGSSETSISLWNAGDGSFAPVNDGFLANGEVLSLDFRPDDQWLAVGDST
                     GFVYIFDIGLHEEIARLPHVDKVTSISFSPDGSAISTAARKTVLFWGVSSIPQILSKD
                     LIENACSRLTHNFDQSKWELLFSDEEYRPICPNLAVEN"
     gene            11206..12537
                     /locus_tag="PL000_15110"
     CDS             11206..12537
                     /locus_tag="PL000_15110"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HNC90267.1"
                     /translation="MENQPQQNTPILEVAWTRFAQLDAMATERSKAHLRLRRWIATLG
                     VLTTFFAILTQLFAERLNPVAALILKIIFIVTPLAASALAAYVSQFYSTGDWLVARAG
                     AEEILKEIYAYRTILKGTPTRRTWLENRLGEIQRSVYRGMNGEMILKPYKGMLPPPPR
                     FDPKYPNSDPGFNDLNGDEYFNYRLDNQLSWHIKEINKRQTERTRLKIYILLAGLAGA
                     LFAAIGSSLTMWVALAAAFSAAFIGWQELRSLDSVVRNYSKVVLELNILFDHWKNLEG
                     EERDQTEFYKTVRSTEDILWSQNVEYIKAMQEALRDSDLEEEASLINRVIKEQRESDK
                     RFRGAIADAVVDHTRESMQETEETLVETYKSTLGTLAEEASSDLVQAELAAMREAAQN
                     AVENFSEKIGLSSAMQAIHDEFAGVDIDANTPMSVLNDLMSRLPKTTEVKG"
     gene            12554..14956
                     /locus_tag="PL000_15115"
     CDS             12554..14956
                     /locus_tag="PL000_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HNC90268.1"
                     /translation="MTETTPTPTAQSTPAAEAGKEAQKQAGNEAGTATTTPAPAVAAT
                     PIVASTTAEQTAKKEKEPEKSSTLSELRSSESSHPVRPEVRPHAFVVMPFGKKKGGDG
                     SLYDFNAIYKELIKPALEEAGFEPFRADEETSSGDILTDMFQELLLADLCLCDLSIDN
                     ANVYYELGIRHAFRKRGVVHIQAGRAYMPFDIFNVRTLPYHITPEGVPDPQYIKADIK
                     AIARMTRDTWASDRDAIHSPIFNLLTGLREPERTALRTPLATGFWREYNEWRNKVTVA
                     QRQKRIGDILLLTEEIQNPLIKEEAIGEAGRALSDMGRNELALAQYRKGLEVNSSNLN
                     FRRQEAFNLNRMGRVDEAIVKIESLLNDFPNDSEAIAYLGRIYKEMWVGSWKNINDKN
                     KRIKNAFETYHWLIRAVDIYLRGYHIDLNNNYPGINAFTLSIIAVHLADKFDDPKDPD
                     PDITRIRQNLPELRGSLIFGLENITDDDRADYWTLISLAELRVLTADNVSAVARAYRK
                     AITASRRNLFFLNSSLSQLEILKALGLREEFVQVGIDTLKDEIMRTSGDDASHSTSKK
                     GRSKKTKTDARAFLFTGYMVDYPAKEKKTFPPERENEIRQEIIRKLEKFKITPDDRAF
                     VGGLSAGSEILFAEVCAEKGIKVKAFLPLPESAYIRKFVSPAGDNWVDRFHKIRNHPL
                     VDEMYQIEHVGLPKEGDDPYERNNRWALYSSLGKGIDKVYLIALWNAHGDKPKDLDAR
                     LVHHMIELMRDTGGSIEQINTSKYLHSVIDNVLGRLMDEQESGKAKSNATKVRKLSDS
                     KK"
     gene            complement(14990..15460)
                     /locus_tag="PL000_15120"
     CDS             complement(14990..15460)
                     /locus_tag="PL000_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90269.1"
                     /translation="MKEPIILKSDDPILNTIKFKPYRNMVQRRVVPFVPGEGQPQTME
                     VITPWGARLVAKKGDLLVGELNAPDDMWPVSPEIFDASYMVISPGICVKRAVTLLAPL
                     TDLTDGDEDQTVIIHTLEGVDTVRAGDFLLAKGVKGEIWPYPKAKAAEIMRPAE"
     gene            15540..16889
                     /locus_tag="PL000_15125"
     CDS             15540..16889
                     /locus_tag="PL000_15125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNC90270.1"
                     /translation="MRHSFIRYNFILVSAILLSSLACSSLTPTPEPALTYRYDNLPNA
                     GEEDETAARFRAISQWGKTNLTYYFANGTSKINGNIERDLIRAAFQLWADETPLTFNE
                     VSDSAQADIIIGWAEGEHGDGDPFDGPGDVLAHASYPSPYQERQVFLHFDDAERWVDS
                     NTQNVDLLTVAAHEIGHNLGLDHSNDPNALMYPSYSGPHRFLGNDDVAGVQSLYGLAA
                     QPAESPETPPQGATPPPSANQDSDGDGISNTDEALRTGTDPNNPDSDADGLSDGVEVI
                     YRMNPLDADMDNDGVNDGQEVAQGSNPFFPDQTASISPELSAEVSEFLTKAIELQIEA
                     YRRGDSSIASSIMAGDIFTTLDQDITTLNGQGFAQISEIDYYNSYINDIRVINETNIE
                     VDTCEVWSTNTYRRADGKLIQSTDPRLLPQTITIQKLSQGWFITQVNFFEAPSFCNS"
     gene            16897..17496
                     /locus_tag="PL000_15130"
     CDS             16897..17496
                     /locus_tag="PL000_15130"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC90271.1"
                     /translation="MNKLIVIVGASGVGKTTLARALCSKQNFALAYEQHNERPFQALF
                     KNDPKYALANQMDYLLYRAEQEYEFRQGDLPALMDGGFDLDFHGFTRLFHTRGLLNDA
                     EYDLCRRFYTLTRSLLPPPDLIIALSADSQTISTRLASRNRINIASSEDANLLEEYVG
                     EWLATVPEEKVLKLDVTHENEEYTQSVPLILDTLDLLHK"
BASE COUNT         4591 a         4794 c         4178 g         4037 t
ORIGIN      
        1 tgaagaatcc cgcgccgcgc ttgattggtt ccaaacctcc gtgcccgaac gcacactgca
       61 caactggcaa aacaccgccg ccaaattgat ctccgatgat ataaaagcgg gccgcgtttg
      121 aaacgcggcc cgcttttttt actgctgggt tgttggaacc acgggcagtg attgtagctt
      181 gagcatggga gaatctgacg cgaaagattg ccagaacgga tcgaacaggt cggtatttgg
      241 aatttcagca aaaagggtca aaccgcttcc atcgaggttg accagataca aggccttggt
      301 tttaatttca gtttccttgt catctgcact gaaaacaatt cgcgcaccgt caggtgacca
      361 gttggcattg tctgggatca gtgaacctgt attcactttg acagagccgc tgccatcggc
      421 cttgccgatg tagatgccat ccttgcggaa gaagagcaca ctttcgccat caggcgacca
      481 gcgcggaaag gcttcgtttt ggtcgggggt cacttccagc ggactggcac taccgtcttc
      541 gatattcatc agataaatat cgttatcttt gagacgcagt gtgaaggcga tttgttttcc
      601 atcaggagac cagtttggag aatagaaatt tgcttctggt tgttccgccc gcaaagcagc
      661 ggtcaataaa ttttgcgtac ggccatccga actcgaaagg atgatctctg aaaacgcaga
      721 ttcgtttcga gaatcgaagg ccaggttttg cccatccggc gaccagacag ggcagccttc
      781 ttccgcctcg ttgtctgtaa ttcggcgtag cgtcgaatcg ctgacggtca tcacgaaaat
      841 atcccagcgg atatcacgtt tacgaaaggc gattttggtt ccatcgggcg aaatggcggg
      901 gcaaaatgca gaatacaaac cttttgtcaa ttttgccatg cgcccgggag aggcggggtc
      961 aagcacgtac agatcgaggc catcaagtac gttcccgcca acagccatga tcaactcgtt
     1021 accggcaacc agcccacccg aagtcgggag cgagcgggaa tcagggggaa catcgtcaaa
     1081 gcctttgaaa ttcacttttg tattatcacc acttcctgta acagatccgc tgcacccggc
     1141 aaagacactg cccgaagcat cacccaccaa aaactcaccc ttgagcactt cgcaatgaac
     1201 cgtcccaaat tcggatgcat tggtagcgta caaagtgaat tcggcggaat tgcccagatc
     1261 cacttcaata ctccatggca attgaacatc ctcttccacc atgcttccat cggggctggc
     1321 atattccaca tgtgcctgcc cagccaaacc gttgacacga taggtatatt tttctccgcc
     1381 cccgcagcct gtcagcaaga acaaagctgc caaagcaaaa accaatgcaa ctctcccatt
     1441 tacgattctc atctcatcct ccttggcgga atttgaattt caaagttcgg attatacaag
     1501 tcaatttaat aactggcagt acatggcagt tcatttcgtg gactcggcag tgcgcgattc
     1561 gtacactggg ggcaaaggag aaactcatat gaatacaaaa aacaatctca atttttggtg
     1621 gcgctggttg gtaactgcca acatcggcgt gattcttttt gggctcacac aggccctcct
     1681 gcccaatctg gtacgtcagg gctttagttt gatggtcttc ggctcagcct cttatataga
     1741 ttcatttcca ctcgacgcaa tccgttacat caccctcacc catgcagtga tgggcgccgt
     1801 catgatcggt tggggtgtat caatgatgta cacactcttc acccaattcc gcaaagggga
     1861 atggcatgga tggctcagcc tcgtggtggc actcatcctc tggttcatcc ctgataccgc
     1921 catgtcgatt gcaacgggct tttggcagaa tgccgtactc aatacagtgt ttgcaatcct
     1981 ttttgtcatt ccactttctg caacctacca aattttcaga agggcataaa caatgaacaa
     2041 tcaactcaac ctcagccgca tcttattggc caatggtgtc ttctgctcca ccagtggatt
     2101 gatcttcctg ctcgccgcca aacctctatc ggaattcttg aataccgttc cagttgtgat
     2161 gagcgttttg ggcattggat tgattgcata cggggtatgg gtgatttaca tggctacacg
     2221 ccgtttgatc actaatttct tcagcatatt cgttatcggt gcagatttgc tctgggtgct
     2281 gttcagtgtg cttttattga tactcccagc tttcaacttc agcgcagaag ctaaatgggc
     2341 cattggcatc acagccattt gcgttgatct gtttgccacc gttcaatttt tggagtggcg
     2401 taaaatgaga tgagccaacg agcaagtgga aaatatgccg tttcttatta cattctccac
     2461 ttgctcaacc accgtcggtt ggctatgcta tagtcaaccg acggaaccct gtccggtatg
     2521 cgatcaaaga aaagcgagcg tgaagcatgg ccaagatact ggtcgttgaa gatattcagg
     2581 ataattttga acttgtacgg cgagccctct ctggcaccga ccatcagatc atccacgcac
     2641 ccgatgcgga aactggcctg caatctgcgc tggataccat ccccgatctg attctgctcg
     2701 atcttggtct gcccgatcat gatggtcaaa cgctggcggg gtggctgcgc gaaacccccg
     2761 aactggccaa cacccccatc gttgcactct cagcctggcc cgaggaaaat gcaaaagaga
     2821 tggtggaaag ttatggcttc agtggataca tcagcaaacc cattacccgg gttgccgatt
     2881 tcatcgccag aatcaattca tttctcaaag cccaataaat gcaaacccct gactcgaacg
     2941 aacccattca attaatccct gcaagccgtt tcaattatga agaactgaca gccatctaca
     3001 accaaacacg cgtcgactac atgatcccca tgccgatgaa tgcggcgcgc ctcgcagagt
     3061 acgtgcgcat ctacgatgta gatctggatc attcactggt ggcggcaacc catgatgggc
     3121 agatgctcgg ggttgccatg ctgggcattc gaaaaaaacg cgtctggctc acacgccttg
     3181 gggttctgcc caacacgcgc cgcagtggaa tcggcagcgc attggtgggc ggtttgatcg
     3241 aagatgcagc aaaacttggc ctcgattttg tcatgctcga agtgatcaag aacaacacac
     3301 ccgcacacca gctttttttg aaatatggct tccgcgagat cggtgagctt ttggtcttgc
     3361 ggcgttcccc gctgattcca cccattgacc cgatcgtggc agatgcccaa cgtatggatc
     3421 gtactgaagc cctggtgctg gtcggtcacg accgcggcac ccaaccctgg accaaccaat
     3481 ccgaatcctt gatcaatgcc catgaggttt cgggtctgag tatcacgctg gtggatggca
     3541 gccgcggctg gctggtatat caacgcgaaa aattcctact tacccgtttt gccattaaaa
     3601 cagaagcagg cgacccagcc acggtcgcct acgctctgtt gacacattta cattcccagt
     3661 atccgcgttt ggatactcac attgaaaata ttcaggttaa cgatccgcac ttgcctgctt
     3721 attacaaaat ggggtatgta gaatcgttcc gtcgcattga aatgtggcgc ggtgatttcc
     3781 cttccagcaa ttgaaaggct tattttctac gcacccacag cccaagcgtg cctgcaaatg
     3841 actgcatgaa ttcatcgtag gcatattcct gacggcggtt tggataggga ttgtagatct
     3901 caacccagcc gccatttccg ctgtttttgc cattggggaa aaccttatcc accacaaccc
     3961 agtgcccaat gccctgtccg ctcaatcggc ctgtcagctt gtcgctttgt cgaatccgca
     4021 cacccgctac gaggtagtgg gtttcgagca ttttcttcat cctgcctgca gaaagaatga
     4081 ggatgccggt cacagggtct ttcattccca attcaaaacg aaaaacatcg ccgggggctg
     4141 cattgtaggg agaagcccga aacatggtct ccagtgaatc aatgccagtg ccattatcat
     4201 tctttcccgc aatggacacc tgataatacg gcctggcctt gagcttccaa tcctgcacaa
     4261 aactttcaac atcgatattc ataatgaaag aggcgcacaa ctgtccgcac atattgcgtt
     4321 ttttgccgtc ttcaccaggc aggaacatat attgttcagc atcatcttcc tccggtgtgg
     4381 gatgcgggat gatgacttcg ggagttgaga aacggtcttc gtaatcttca agaacggaat
     4441 ttcgcaccca acccgttcgg ctcttgtatt tgacctgtat ccaggtcttg ccatttttga
     4501 cttcggtggc gcctgaagat tccatcactg cgcccttcat aaaactcaac accccaacac
     4561 catcgggggt ctggcggaaa gtaccggggg caatgcacca tttcttggcc gacgcaactt
     4621 catccattgc tttgacggcc accatctctg acctgcgttt gacccagcac cccgtgtatg
     4681 tgccgccccc aaaggaacta atgaaatatt cgtaactata ttcctggcgc tgattattga
     4741 acgggttgta tatttccaca cgcccgccat ctgtgccgtt tggtgtggcg ctttccaaaa
     4801 cgatccaatg attaataccg ccattcgcgc gtttggtctc gttgttgggg atcaacttgc
     4861 ctgtattttt gttgatgacc acctgagcca gcagggaatg tgtttgcaac attttctgga
     4921 agcggccagg gctcaaagtt ttgtcttcga aggcggggcc gatcaacgta actgcaaagt
     4981 caatgatatt tccgttcgcc gcgctgtaag agtagggatt cagcatgttt ttgacgaggg
     5041 agaggtcgga ggttttgtca ctgtcaccgc ccagtgccca cttatacaaa ctgccggcac
     5101 tgttctccca atctcgcagg aattgtttga tcccaactcc catgacaaaa gcgacacaaa
     5161 attcggcgca caggttgtat ttgacctgct cttcgccttt gctgttcctg cctaatacca
     5221 gatattgggc agggtcgttg gggtttttgg tggcataggg aaattcgccc atctgctctt
     5281 ccgtggccgc gggaatttca accacgggtg gaggaaattt ctccacgtag tcatcgaaga
     5341 attcatcacg cacccaacca gcaacggtgc cttgtgagtt acggtaaaca cattccaccc
     5401 atgtggaggt gtaggtttgc ccatccttgc cgagcatggg aacgtcttcc tgtcgtcctg
     5461 tcacttcaac taatgcccca agatatattt cagtcacggt ggcacccttt ggggattcac
     5521 acagggtggc cttattaata acgcaccatt tttggttcgc catgctttct ccagtcgatg
     5581 ttttttatgt ttggcggaac aaatttcccg caattatggc acaggaggtg gatggaggtc
     5641 aacatacaat tctcacaagc aatttcggca aatttacaac cgcaacaatt tcacaacaaa
     5701 atcccttttc agccattttt ggcggcaaaa tatcctcaat ctaatccatc acccaaaagt
     5761 tctatttcag ctcaaaatgg taaggattat ctcttgttcg tgtacaatag ccaaaaggtc
     5821 agcgatgcgc attgctttta tttcagacat tcacggaaac ttcaccgctt tacaagcggt
     5881 tcttgatgac atcaaggtac agaacatcga tgaggtcatt tgtcttggtg atacggtcag
     5941 ccttgggccg caacccaggg aagtgcttga ccgcttgcgt gaattgaact gtgtcattat
     6001 caaggggaat catgactccg ctattttgga ccctgaagaa gctgaaaaat ttgaaattac
     6061 cagccatctc atccccgact tgtattggtg cagggaacgt ttgaccaaaa acgaccttga
     6121 ctttattgcc tctttcaaaa cgcttcacga aatcagcatg ccgaatgggg tcaaaatttt
     6181 gtgctttcat gggtcaccgc tttcgcccac cgatatcatt caggcaacca ccccacccga
     6241 gacactggat aaatactttg aaggccacca ggcgaccgtg tttatcgggg gacactctca
     6301 tattcaaatg tatcgccgcc atggggataa actgattcta aattcgggca gtgtcggaaa
     6361 ttcgttcatg ttcgcattta ctccgggcaa tgcgccgcgt ctgctgcctt gggcggaata
     6421 tacgatcatc agccagaacg ggaattcact ggatgtggac ctgcgcaggg tgtatttcga
     6481 cacaacggtt ttgatggagg ctgtacaggc cagcggcatt cccagtgcag agtggtggtt
     6541 taggcagtac gcaacaacga aataagctca ttatttacgg ggaaatcata cggggagaaa
     6601 aatgcccatg atgggctgtc aggcatcggt ttgggggtga agcgcttttt tttccacaat
     6661 aaacctaaca aaattacaac ttcattgcga atcgaaatgg agtaaaattg ccgttaatca
     6721 aatttcacaa cttaacaaat gcaggaggta agccgatgtt cgttaccaag aatctctacc
     6781 agaacgacga aacgtggaag tctactcctc tcggaaattc cagtgaaacc atcggtggat
     6841 ggggttgttt gctcacatcc gtcaccatga tgctcaatgg gattggctac aatgagaccc
     6901 cggtaaccgc caatgagaag ttcaaagctg caggtggttt tagcggggct ttatttatgc
     6961 cgagttacct cccctacgtc tggccgaatt gcgcctatcg cgacatgcag ccctgtgagg
     7021 cttaccctgc acccattccg cagattgatg cggcggtggc ggcgggcaag cccgtcattt
     7081 tacaggtgga ttggaacaag caggctggta ttcaaacgca ttttgttttg gtgaaggaaa
     7141 agaaagggga cgattacgtt ttatacgacc cctataagta cagcggtgac ggccccgaca
     7201 aggaagtgct gctcaccacc cgatacaaat acaatggtgc aaaactcacc tcagagatca
     7261 gtgcggtgtt gtggtttgac tcatacagca gcacaccacc tgaaccgcca aaagtgacca
     7321 aggttcccgt acctgcggag aagtatctcc tttacgcagg tgaggatgat cttgccctgc
     7381 gctctgaacc ttctgtaagc ggctatcttt ggaagcgtat gctgctcggt accgagttga
     7441 tctgcctgga agacaaggca aaggccaaat cgaagatcgg tgtacaaggt cagtggatcc
     7501 aggtacagga tccgaagggc gatcagggat atgtggccgc atggttcgtt tctgagcaaa
     7561 aaggaaagcc tgctgcttcg accacgacca cctcgacacc tgctgctgct ccagcacccg
     7621 ttgctgccgc cccagcccct gttcctgctg gtgcgctggc tctatacccc actgaagaat
     7681 tatccttccg ttctcaacct gtgatttcac ccgagactct tgtccgaagg gttccagtca
     7741 cagaacagtt gatttgtctt gaagctgcca atacggccat tccgaaggtg ggtgtacaaa
     7801 atcaatggct gaaggtgcgc ggtgcggata agaaggaagg ttatgtagcg gcctggtacg
     7861 tcaagtatgc gggcggttcc actgcacaag cagcggctac atcggcccct gcagcgacag
     7921 gctcgaagaa ggtcaagacc acagttgaaa tggtctccct gcgtaatcag cctgtagtga
     7981 gcgacgccac cttgatcaag cgcgtgccca tcaactatga attcaccatt accgaagcag
     8041 gcggcgaagc caaggttggc aaaaatgacc agtggatcaa ggtcaaagat gcgaccaatg
     8101 aaggctatgt agcggcctgg tttgtaagtt tctaatacaa agcagacccc atcatggaag
     8161 aaagttcgcg gacaaccaag attttcattt cctattcgcg caaagacaag ctctttgtac
     8221 gaaaattgaa cgcagccctc gatgcagcgg gtattgatgc atgggtggac tgggagggca
     8281 ttgaactggc ttccgattgg atggccagaa ttacagccgc gatcgaaggt ggtgatgcct
     8341 ttgtcttcgt gatcagtccc gattcgctta aatccaaggt ctgcatggat gaattggaac
     8401 tcggcatctc cagcaataag aagatcattc ccgttctata ccgtgagcct gagaaaaaac
     8461 aaaagatgca cccaaaactg gcctccacca attgggtgta tttacggccg aaaaaagacg
     8521 atttcaagaa caccatcccc aaactgatcg aagccattca aacagacctc gagtgggtac
     8581 agcaacatac ccgcctgctc caacgcgcca cggaatggga tcagaaacga cgaaacaaaa
     8641 gctacctcct gcaaggtacc gacctggaag atggcgaaca atggatgacc gcttccacca
     8701 ctggccccaa ccgcagcgtc aacccgatgc aggcggaata tatcagcacc agccgcaaga
     8761 cttcggtaca gcgtcagcgg aatctgacca ttggtgtcgg agccatgctg gtcatcagca
     8821 tctttttcgg catctttgca tttctacagc gcaatatcgc caggcaaagt gaaagggatg
     8881 cggtcaacag ccgttccact gcggttgcaa atgaactggc acgtgccacc cagcaggcca
     8941 ttgcagaaga gcaacgggtg attgctgaac aacaaaagtt aattgcgcag gaaaacgaag
     9001 aaaaagcgga agcgcagcgc agcgcagcag aagcaaaaat atatcaggac agggatggtg
     9061 agttggatac cagcaccctg ctggcggtca atgctctcca aaaactggaa gggttggatg
     9121 acgctgagga tatcctgcgc aacaatatca ccctgctgcc gatcccggtc aaacaaatga
     9181 acctgggggc ccgtgtctgg gctattcaac cgagtgctga ccagtccaaa tttgtcacaa
     9241 cagattcggc aggaaaagcc tgtgtctgga gcatggaaga tggatctcaa ttcttttgcg
     9301 cccagcatga cggaattgtt tacgatgcaa atttcagcca ggatggaacc atcctggcaa
     9361 caggcaccga aaaaggtgtt ctgacctttt ggaatgtaga caccggcgag cagatcaaat
     9421 ccatacaata tgaaggcacc atctgggatc tcaatcctca cccgggcggt cgctggcttg
     9481 gtgttggccg cagcaatgcc atcagcatga tcgatatgac cgatttgacc gaagcgttgt
     9541 ttatcacgct aagcggtgaa gtcaagacca tagattttga tgatacaggt tcctttatgg
     9601 ccatgggtac atccaaagga aatgtaaccg tttgggcggt catgggcaac caatcggtcg
     9661 gcggaccaaa gcacaatggc gaagtcattg atgttgaact cagtcctgat ggaaattggg
     9721 tagtcagtgt cggacaggat agcactgccc gcagcgcgca atccagggca ggcggacaaa
     9781 aatattccgt tacgcacggc gactgggtgg aagatgtcac cttcggcccc gatagttcgt
     9841 ggtttgtcac tgcttcagac gataacactg tcagagttat agacacagcc acaggtcagg
     9901 aaagattgcg catggtccac aataattttg tccaaaaggt gcgtgtcagt ggagacggtc
     9961 aatggatcgc caccacaggg tatgacaaaa ccctgcgcat ctgggatgct gcctccggag
    10021 cagagaccat gcagattccg ttggagggaa tcgggtcttc catccgcttc aacagggatg
    10081 caacccgctt gatcgttggg gattacgatg gacatgtgac cctgtggaat atttcggaac
    10141 tcaaagcccg ccgaggattt cagcagtttt ccgagtttgt aaacgaagcg catttctctc
    10201 cagatgggaa gtggctggcc gtcaacaccg atgaccgcaa aatctggcgc atcgacctgg
    10261 acgaatcagg aaacccacaa ggcgagcgca aggtaattgc cgaaaccgat ggtctgacca
    10321 gtgatatggc gatcagcgcc gactcaaact gggttgcggc ggttgaaaac gacgagaaca
    10381 tcgccgatta caaccgtgtc gttttagccc gtgcagatgg cagcaatgaa ttcttccttt
    10441 ctcacgatac acaagtcatc acttctactg cattcacacc agacagcaaa cagatcatca
    10501 cagccgatga aaaaggcgtc atccacattt ggaatgtgga aactggcgaa aaggtgaatt
    10561 cattgtcagc ggatgggatt attttgaccc tggcggtttc accaaatggc aaatatctgg
    10621 tggcaggcat tgaggacgcc aaccatagtc ttgtctggga tctgaccacg atgacccagg
    10681 ttaaagtact ggaacagatc ggcaagatca aatctgttca attcagcaac aacggaaacc
    10741 tgttggccac aggcagttca gaaacctcca tttccctgtg gaacgcaggc gacggctctt
    10801 ttgccccggt gaacgatggc tttttggcca acggcgaagt gctttccctc gatttcagac
    10861 ctgatgatca atggcttgct gtgggtgaca gcaccgggtt cgtttatatt tttgatattg
    10921 ggctgcacga agagatcgcc cgcctccccc atgtagataa ggtaaccagc atttccttct
    10981 caccagacgg cagcgccata tccacagcag cgcgcaaaac cgtcttgttt tggggagttt
    11041 catccattcc acagatcttg agcaaagacc tgatcgaaaa cgcctgctcc cgcctcacgc
    11101 ataatttcga ccagagcaaa tgggaactcc tgttctctga tgaagaatat cgacccatct
    11161 gccctaacct ggcagttgaa aactagacct attggagaag ccagtatgga aaatcaacct
    11221 cagcaaaaca ccccaattct ggaagtcgcc tggacccgct tcgcgcaact ggacgccatg
    11281 gcaactgagc gatcaaaggc ccacctccgc ctgcgaagat ggatcgctac actgggtgtc
    11341 ctcaccacct tttttgccat tctcacccag ctctttgcag aaaggctcaa cccggtggca
    11401 gctctgatct tgaagatcat cttcatcgtc actccacttg cggcttctgc actggctgca
    11461 tatgtcagtc agttctattc cacaggtgat tggctggttg cccgtgctgg tgcggaagag
    11521 attctcaaag aaatctacgc ctaccgcacc attttgaaag gcacacccac ccgtcgcacc
    11581 tggctggaaa accgcctggg tgaaattcaa cgctcagtgt atcgcggcat gaatggcgaa
    11641 atgattctca aaccctacaa gggaatgctt cctcctcctc cgcgtttcga ccccaagtac
    11701 ccaaacagcg accctggttt caacgacctg aatggagatg aatacttcaa ctacaggctt
    11761 gataatcaat tgagctggca tataaaagaa atcaacaaaa ggcaaactga acgaacccgc
    11821 ctaaagattt acattttgct ggctggcctt gctggcgcat tgtttgccgc cattggctcc
    11881 tcactcacca tgtgggtggc gctggccgcc gccttctctg ccgccttcat cggctggcag
    11941 gagctacgca gccttgattc ggttgtccgc aattacagca aagttgtgct tgagctgaac
    12001 attctcttcg accactggaa aaacctcgaa ggcgaagaac gagaccagac cgaattctac
    12061 aagaccgtcc gctccaccga agacatcctc tggtcgcaaa atgtcgaata catcaaggcg
    12121 atgcaggaag ccttgcgcga ctctgacctt gaagaggaag ccagcctcat caatcgcgtg
    12181 atcaaggaac agcgcgaatc agacaaacgc tttagaggcg cgatcgctga cgcggtggtg
    12241 gatcataccc gtgagagtat gcaggaaacc gaagagaccc tcgtcgagac ctataaatcc
    12301 acacttggaa ccctcgcaga agaagcctcc tcagaccttg ttcaagccga acttgccgcc
    12361 atgcgcgaag ccgcccaaaa tgcggttgaa aacttctccg aaaaaatcgg gctttcctcc
    12421 gcgatgcaag caattcatga tgaatttgcc ggtgtagaca ttgacgccaa caccccaatg
    12481 agcgtgctca acgacctcat gtcacgttta ccgaagacga ccgaagtaaa gggctaacgc
    12541 ccaaggatca acaatgaccg aaaccacgcc cacccccacg gctcaatcca cacctgccgc
    12601 agaagctggt aaagaagccc aaaagcaagc gggtaatgag gcagggaccg caacaactac
    12661 tcccgcccca gcggttgccg ccacacccat cgtcgcatct actacggctg agcaaaccgc
    12721 aaaaaaagaa aaagaacccg aaaaatcttc cacgctttct gagctgcgct caagcgaatc
    12781 ttcgcatcca gtgcggcccg aagtccgacc gcatgctttt gtggtcatgc cgttcggcaa
    12841 gaaaaaaggt ggcgacggtt cgttatacga cttcaatgca atttacaagg aattgatcaa
    12901 accagccctg gaagaagctg gctttgagcc tttccgcgcc gacgaagaaa cctcctccgg
    12961 cgatattctg accgacatgt tccaggaatt actgctcgcc gacctgtgtc tgtgtgacct
    13021 aagcattgac aatgccaatg tctattacga gcttggcatc cgtcatgcat ttcgaaaacg
    13081 cggcgtggta cacattcagg ctgggcgtgc ctacatgcct ttcgatattt tcaacgtccg
    13141 caccctgccg tatcacatca cccctgaagg cgtgccagat ccccaataca tcaaagcaga
    13201 tatcaaagcc attgcacgca tgaccaggga tacctgggcc tcagaccgcg atgccattca
    13261 cagcccgatc tttaatcttt tgaccgggtt acgagaacca gagcgcactg ccctgcgcac
    13321 cccgctcgcc acaggtttct ggcgtgaata caacgaatgg cgaaacaagg tcacggttgc
    13381 ccagcgccaa aagcgcatcg gcgatattct cctgctcacg gaagaaatcc aaaacccgct
    13441 cattaaagaa gaggccattg gcgaagcagg cagggccctt tcagacatgg gacgaaatga
    13501 actcgccctt gcccaatacc gcaaaggatt ggaagtcaat tccagcaacc tgaatttccg
    13561 acgtcaggaa gccttcaacc tcaaccgcat gggccgcgta gatgaagcca ttgtcaagat
    13621 cgaaagcctg ctgaatgact tccccaatga cagtgaagcg attgcctatc tgggtcgaat
    13681 ctacaaggag atgtgggtcg gttcgtggaa gaacatcaac gacaagaaca aacgcataaa
    13741 aaatgccttt gagacctatc actggctgat tcgagctgtg gatatttacc tgagagggta
    13801 tcacattgac ctcaacaaca actaccccgg tatcaatgca ttcacgctat ccatcattgc
    13861 cgttcatctc gccgacaaat ttgatgaccc caaagatccc gaccccgata tcacccgcat
    13921 ccgccaaaac ctccccgaat tacgcggctc tttgatcttt ggcctcgaaa acataaccga
    13981 tgatgacagg gcagattact ggaccctgat ttcgctggcc gaattaagag tgctgaccgc
    14041 ggacaacgtg tctgcagtgg cacgcgccta ccgcaaagcc atcacggcgt cacgccgaaa
    14101 tcttttcttc ctgaattcct ccctctcgca attggaaatc cttaaagcgc ttggcctgcg
    14161 tgaagagttc gtgcaagtag gcattgacac tctcaaagac gagatcatgc gcaccagcgg
    14221 cgatgatgct tctcacagca caagcaaaaa aggcagatcg aaaaagacca agaccgatgc
    14281 acgcgccttc ctgttcactg gctacatggt ggattacccc gccaaagaga aaaagacctt
    14341 cccacccgag cgcgaaaatg aaatccgtca ggaaatcatc agaaaactgg agaaattcaa
    14401 gatcactcca gatgaccgcg ccttcgtagg cggactctct gctggaagcg aaattctctt
    14461 tgccgaagta tgcgccgaaa aaggcatcaa ggtcaaggct ttcctgccat tgccagaatc
    14521 agcctacatt cgcaaatttg tttccccagc tggcgataat tgggtggacc gtttccacaa
    14581 gatccgtaat cacccgcttg tggatgaaat gtaccagatc gagcacgttg gcctgccaaa
    14641 agaaggcgat gacccctatg agcgtaacaa ccgctgggct ttgtactcct ccctcggaaa
    14701 aggcatcgac aaagtctacc tcattgcctt gtggaatgcc cacggtgaca aacccaaaga
    14761 cctcgatgcg cggcttgtcc atcacatgat cgaattgatg cgcgacacag gcggttccat
    14821 cgaacagatc aacacctcaa aatacctgca cagcgtgatc gacaatgtcc tcgggcgcct
    14881 gatggatgag caggaaagcg gcaaagcaaa atcgaatgca accaaagtgc gcaagctctc
    14941 tgattcgaaa aaataatccc taatcccact tcaaaaaaag acccgccgat cactcggcgg
    15001 gtctcatgat ttcagcggct ttcgccttgg ggtaaggcca gatctcccct ttaacacctt
    15061 ttgccagtag aaaatcacct gcacgaacag tatccacccc ttcaagggtg tgaatgataa
    15121 ccgtctggtc ttcatcgccg tcagtcaggt cggtcaacgg cgccagcaag gtcacagccc
    15181 gcttgacaca gatccccggg ctgatgacca tgtacgaagc atcaaagatc tcggggctta
    15241 ctggccacat atcatccggg gcattcaact ctccaaccaa cagatcacct tttttagcca
    15301 ccaaacgcgc cccccacggt gtaatcacct ccatggtctg cggctgaccc tcacctggca
    15361 caaaaggcac aacccgtcgt tgaaccatgt tacgataagg tttaaacttt atggtattca
    15421 agatcgggtc atcagatttc aatataattg gctctttcat acacctctcc gtatcaatga
    15481 tcaacaaacc aattgtaacg taaaaaccaa aaccataaat caaaaatgag gagacaggca
    15541 tgcgtcattc ttttatcagg tacaacttca ttctcgtttc agccatctta ttgagttcac
    15601 tggcctgctc atcgcttacc ccaacaccag aacccgccct cacttaccgc tacgacaatc
    15661 ttcccaacgc tggtgaagaa gatgaaaccg ccgcccgctt tcgcgccatt tcacaatggg
    15721 gaaaaaccaa cctcacctat tacttcgcca atggaacctc aaagatcaac ggaaatatcg
    15781 aacgcgacct catccgcgcc gcttttcaac tttgggctga tgaaaccccg ctcaccttta
    15841 acgaagtatc tgattctgct caagcagaca ttatcatcgg gtgggctgaa ggcgaacatg
    15901 gcgacggcga tcccttcgac ggccccggcg acgtgcttgc ccatgcatcc taccccagtc
    15961 catatcagga acggcaggtc tttctccatt ttgacgatgc cgaaagatgg gtggatagca
    16021 acacacaaaa cgtagacctg ctcaccgtag ccgcccatga gatcggacat aacctcggcc
    16081 tcgatcacag caatgacccc aacgcgctca tgtacccatc ctactcgggg ccgcatcgct
    16141 tcctcggcaa cgatgacgta gctggtgttc aatctttgta tggccttgcc gcacaacccg
    16201 ccgaatcgcc cgaaacaccg ccgcaaggcg caacacctcc cccaagcgcc aatcaagaca
    16261 gcgatggtga cggcatctcc aacaccgacg aagccctgcg cacaggcacc gaccccaaca
    16321 accccgatag cgacgccgac ggcctcagcg acggtgtgga agtcatctac cgcatgaatc
    16381 cgctggatgc cgatatggat aatgacggcg tcaatgacgg tcaggaagtg gctcagggca
    16441 gtaatccgtt tttcccagac caaacggcga gcatttcccc cgagctatct gctgaagtca
    16501 gcgaattcct cacaaaagcc atcgaactgc aaattgaagc ctaccgccgc ggcgactcaa
    16561 gcattgcatc ttccatcatg gcaggtgata ttttcaccac gctcgatcaa gacatcacca
    16621 cgttaaacgg gcagggtttt gcccagatct ctgaaattga ttattacaac agctacatca
    16681 acgacatccg cgtgatcaac gaaaccaaca ttgaagtgga tacctgcgaa gtgtggagca
    16741 ccaacaccta ccgccgcgcc gatggcaaac tcatccaaag caccgacccg cgcctcctgc
    16801 cgcaaaccat cacgatccaa aaactatcgc agggttggtt catcacccag gtcaattttt
    16861 ttgaagcacc ctccttttgt aactcgtaga gattccatga acaaactcat tgtcattgtc
    16921 ggcgcgagcg gtgtgggcaa aaccacactg gcacgcgccc tgtgcagcaa acaaaacttt
    16981 gcccttgcct acgaacagca caacgaacgt ccctttcagg cgctcttcaa aaacgatcca
    17041 aaatacgcgc tcgcaaacca aatggattac ctgctgtatc gcgccgagca ggagtatgaa
    17101 tttcggcagg gagatctccc tgccttgatg gatggcggct tcgatttgga cttccacgga
    17161 ttcacgcgcc tctttcacac ccgtggattg ctcaacgatg ccgaatatga cctgtgccgc
    17221 cgcttctaca ccctaacccg cagcctgctc ccgcctccag acttgatcat cgccttgagc
    17281 gcggactcac aaaccatcag cacaaggctt gcctctcgaa acaggatcaa cattgcgtca
    17341 agtgaagatg cgaacctgct cgaagagtat gtcggtgaat ggctggcaac tgtccctgaa
    17401 gaaaaagtcc taaagttaga cgtcacccat gaaaatgagg agtacaccca aagtgttccc
    17461 ttaattcttg atacacttga cctcttgcat aagtaaagtg ttgtagacca caaagtcaca
    17521 aaggcactaa gcttttttct tttgcaagag ttctactcaa aaaaggagct aaaaatgagt
    17581 aacgacccat ttatcgaaaa
//
LOCUS       DAOQLC010000093        17532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1146_length_17532_cov_23.392573, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000093 DAOQLC010000000
VERSION     DAOQLC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17532
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1146_length_17532_cov_23.392573"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1064
                     /locus_tag="PL000_15135"
     CDS             <1..>1064
                     /locus_tag="PL000_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HNC90272.1"
                     /translation="TAYQGIYKLPPAHTLVWENGAARIQKYWDYNYLPKHTASEDELI
                     EELRARLRDAVKMRLLSERPLGAHLSGGIDSSIIVALMSELASGPVKTYSAGFEEQNF
                     TELPYARAVAQKYSTDHHEFTLTYGDIPATLEKIAYHFGEPFADASAIPLYHLSVMTR
                     EHVTVALNGDGGDEDFAGYQRYWLDGFANAYARAPRFLTRSLIPSIANFLPDNADRPV
                     GQSLVNGIKRLEQIPEIDRRASILRWGSYFSPRQRSALWKPEFRFDSANAQNLLAKQF
                     DSAQGSYLDKTLYTDLHTYLPGDLLVKADRMTMAASLEGRSPFLDHEIVEWAARLPDQ
                     FKVKGRSGKYLLKKAFAKELP"
     assembly_gap    1065..1164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1165..1457
                     /locus_tag="PL000_15140"
     CDS             <1165..1457
                     /locus_tag="PL000_15140"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HNC90273.1"
                     /translation="KGRSGKYLLKKAFAKELPDAIKGRGKQGFGIPVSAWFRGPLYDW
                     SKQMLLAQGSSLHQWFDAGALARLIDEHKSARVDHGKRLYALSMLAVWANSL"
     gene            1459..2451
                     /locus_tag="PL000_15145"
     CDS             1459..2451
                     /locus_tag="PL000_15145"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC90274.1"
                     /translation="MTDSPKKFPLWNIAKIILALILVWFVLSKTDLAQLYALRERISL
                     PWLALTLLLYFSLTLLKALQYYFLIGRRVDYPQVLNIVVVQNAISNFIATSAGIASYL
                     ALFRVEQGVKVSRAALAFLLAKVGDLISIWLFMLVSSLLVWPLVASFHSLILLSLSFI
                     GVAILGFFAAVFLRQKFVSVLAWFVEKLKLSRLGFVNKLMDLLASLAEQEHGFVFRMV
                     GTGIAFSLIYMTVTMLWLYASLQTFSFEIQFLQVVFVNIFMQLISYLPIQVFGGLGVN
                     ETTSLYLYSVFNFPQTELAATLIGTRLLFYLTNLVVLLYLPVHTLFFSRSAQKQ"
     gene            2490..4835
                     /locus_tag="PL000_15150"
     CDS             2490..4835
                     /locus_tag="PL000_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90275.1"
                     /translation="MQNASPKNIIQLLTVGLVSGLVFAFLEIKALWGPDQIWNKLLTP
                     LGVAAIIAYILLALVGVFILFLSLWNPSPLRALAQKIKSLRWLVAFATIGFLAWMYLF
                     SPWQATLTIPWTQFIVAAALARLLAWCFVSEQDQPFGWSDLALAFSIFLYPRIVQEIR
                     QYLPAPLLTRGSIAVGFVFQFALLAALYESVGKSLLAKLIELRARLEPVRLILAALFL
                     LAPFIYRYSTGPKEYILYFNIRFMVALIALWFAAYLLCKDSSRLISPESFGVAAGWLV
                     LVSAITRSLLLVVDDPFGLYWSEGNRLYDYSLVFGQDLYNYAGRIPDPYNTPGRYGLW
                     GVLYLWQGLPIWAHRLWNVALLTVPSFVLAWALTKKLSPSFLRQMVFLWVTAFFILLA
                     PLHPPFMIMTVIVALFIFHPSPYVRGASLVAASLYAGISRWTWVFAPGAWGALTDLLL
                     FYPKREGNWFKRLLPTVLMAVLGAVPGFLLNIGNFLGYTTGENTTAQQPLLWYRLLPN
                     LTLGPGIVLLILLTTGPLLVLLAYKMSTRQWKLDGFQLLAVWGALIGFFTIGLVISTK
                     IGGGGDLHNLDMYIGTLIVVTMLGLTGQVEGQDVKRWSSSALTMVCLLAILPIYPFTP
                     FHPSAGSHAWLDLPTREDTDKALKVINDSVANYSQAGEILFMDQRQLLTFGYVTAIPF
                     VPEYEKKYMMDQALASNETYFRPYYQDLANKRFALIVTEPLKVDLKEEGGVFSEENDL
                     WVKWVSAPTLCFYEQVHADKTVGIALLVPRENPVGCEKYLK"
     gene            5035..5718
                     /locus_tag="PL000_15155"
     CDS             5035..5718
                     /locus_tag="PL000_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90276.1"
                     /translation="MSGVTASGKSLIGKRVLVFGYGGRFAVGVELLRRGAAHVVLCDH
                     FVLLDKERNLELLPVYPEYLVGENDNVRPRAEYITLLHGDIREDAIQKQITEVDVVLS
                     TSVYEHLDDVKGITHALAKLTASNGFHLHFVDLRDHFFKYPFEMLTFSEGVWRNFLNP
                     TSNLNRFRLGNYREVFDAYFRKVEIKILERLEPEFRAIRARIRPEFLTGDEAVDSVGL
                     IQVIAEMRK"
     gene            5763..7406
                     /locus_tag="PL000_15160"
     CDS             5763..7406
                     /locus_tag="PL000_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNC90277.1"
                     /translation="MQQRFSSFIIRNEKLIPVFIFLLFLAASLPGVDWGVPALWNPDE
                     MIWRVDSALRGEIIFDETEPDFNYPSLPKYVMFGIGKIVYGLGGTPSDFYISARSFSA
                     LLGAVAGVLVYFMARSIGVDVLTSALAGLLYVASGVAAANGRFAHNDLYLQLFSVLCV
                     YFVIKYQFTKARLWLYGSFFSVGLAASSKFTGGSLLLVPLFVFVFMNWSEVRRNWLQS
                     FEMLFIGGALSFGGYILGTPKALLWMAYYFKRVIPALQRYPQYGFNSGTPIGLFGQWP
                     VFEDAVGTFAYYIFILCFIWFVAKIILWKLGRLQMDETQAQAIMILAATVLLFDLPFM
                     ISINYIPRYFIPFVPFLSILGALFIKEIVEIAKAKNWKYVPQIVSVLLVVGITYSMLR
                     LASTALLFMNDARMPASEFVANLPGEGKTIEYTLYPPIVNKKQFAKAYNYPVFFLKYP
                     TDVVPTGGRYEYNQGEAGLIDRETDYLIIDTLTYGRLYTESVCVTNPVECDFFKRLVA
                     GEIKTFRLMKEFTYTLPPWLPDISLSAVNPEVRIYERVR"
     gene            complement(7453..8094)
                     /locus_tag="PL000_15165"
     CDS             complement(7453..8094)
                     /locus_tag="PL000_15165"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90278.1"
                     /translation="MPPKLKLLPASQYKGVNQYDPIKYYYWPVFGKMYRQRVELALGE
                     CTGGERVLEVGFGTGLAFPNLHDMYKEIHGIDLTADIHAVKSVFEPTGIPLFLEKGDV
                     LKMPYQDNFFDTVLLVSILEHLKPAELKQAFAEVNRVLKPGGQMVYGTPVEKPFMVFM
                     FSLLGHNIRDEHFSTEKEIEEAAHKAFSKGHVIHMQSTPPIAGSVYTVGNFVK"
     gene            8175..8534
                     /locus_tag="PL000_15170"
     CDS             8175..8534
                     /locus_tag="PL000_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC90279.1"
                     /translation="MLFEDMRQWRIMVLMIVVETHIFTRQVLLLLSDEEYRLLQAVLV
                     NRPESGVIIPSSGGLRKMRWGFLGKGKRGGVRVIYYWAVRQERILMLLIYPKNVKDDL
                     TREQLKILRNIVKEEFP"
     gene            8531..8827
                     /gene="nadS"
                     /locus_tag="PL000_15175"
     CDS             8531..8827
                     /gene="nadS"
                     /locus_tag="PL000_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HNC90280.1"
                     /translation="MKEDLFNELVVSVREGGAILRGEKKPARAFKITAPNIKRIRAGY
                     KLSQQEFAALLGISVATLRNWEQGRRMPEGPARVLLQVAARYPDVVWDVVKPIR"
     gene            8857..11376
                     /locus_tag="PL000_15180"
     CDS             8857..11376
                     /locus_tag="PL000_15180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC90281.1"
                     /translation="MPFSSSKKWSALFAVWILHGVVALWQFLSLPSEGLSLQRIVMLG
                     LLLAWVAFAMFLAFSAFRQLHWLNGLLLRINKPFIRDGIFIAAVLALFVRISLMFLLG
                     LAAQTPEFHYGAYVERLSPFLDLLAFVAFEIIVLNLFFVFREGTEYFGLIKSISAKLF
                     IGLVLIGGVVLYISKTDMGIAPIYKGDWARGLPAVPLLEWQIFLAVLFCIGMFLAEVD
                     GRLLKIPSLDLWIALAVWVATVGLWLSQPTVPNASALEPREPNYEIYPFIDAHTYDKF
                     AQSVLIGNGFGPNEIPQRPLYIVFLVFMHTVVGQNYENVIALQTVFFAAFPVVLYIFG
                     REFFGRPIGVSLALLAALRDFTSNLVSPITGNISYSKLYLSEIPTAICLILFLLIGIR
                     WIRSGFPLYLGFLLGGVLGIGMLNRTQVVVALPVLLFFAFILQPKKLLFIIKGTLPML
                     AVIALVVAPWLWRNWQMTGKIIFDNPASQTANLALRYNRLNGEDVDILQKPGESNSDY
                     NDRMVAMANHAMSVNSQGIFKAIASSFINHGINNILLFPLRNDLATPADLITPTTPFW
                     QQWEGRPNFLQSLLLAFYVFLFSLGVTMACQRNGWLGLLPLMVNLAYNLWTSIALLSG
                     QRFMLSMDWSIAMYDLLGIFALLSIFFYALENGRAKILKWYEANTVSIIPTLEAIKWP
                     RYVFAGLMFFAVGASLPLSEMSFSQRYPALEQNQPADLTLLSSLAQTDLEPACQKLAE
                     SSGLEIVRGRALYPRFYSAGDGEIFTDSVGYKKVDEGRLVFDLLGQKNHRVIFPMSQS
                     PEFFPNASDVTIWFDKNASPWFILVEQGDAQRFYVSDRVCK"
     gene            complement(11442..12875)
                     /locus_tag="PL000_15185"
     CDS             complement(11442..12875)
                     /locus_tag="PL000_15185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90282.1"
                     /translation="MKTTPLFDEAGMEYQKTRIAHWDKVAKKRDAWAGLGRWYHRRLI
                     QIYKYLVNPNQRILEIGCGMGSLIAHLQPSHGVGVDFSPEMIARAKARHPEIEYHQLD
                     AHDLSSLEGTFDVVIFSDSVNDLWDVQRALEQVKKFCTPHTRLILNFYSHLWQLPLTI
                     AQGLNLAAPMLNQNWLTPEDVDNMLRLAGFESIRPMREVLWPLPLGGLADRYLVKLWP
                     FRDFALSNFVVARPAATPVKNPSVSVIVAARNEAGNIKNIFERLPRMGSKTELIFVEG
                     HSRDNTFQTIQDEIPLHPSTPSLLFQQTGIGKADAVRLGFEKASGDILMILDADLTVP
                     PEDLPRFYDAIASGKGEFINGVRLVYPMEKEAMRTLNFIGNKLFSMAFSWLLGQPIKD
                     TLCGTKVMWREDYEKIAANRSYFGDFDPFGDFDLIFGAAKLNRKIIDLPIRYRDRTYG
                     TTNISRWKHGILLFRMVAFAARRIKFV"
     gene            13044..13691
                     /locus_tag="PL000_15190"
     CDS             13044..13691
                     /locus_tag="PL000_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90283.1"
                     /translation="MKKTILAILTLALVLTSCAKAKDTEVAPVFTGGSSTVEWASKAA
                     TVEELVSEADLIVRVRVIEAPVTRVLRHELPVWGENNKIVGSETFEALFSDTVFEVVK
                     TYLGDPPSQITVMQIGGYDPTVSKGIEEVGDDPLYKVREGYILFLVNISGDQIHAPDR
                     ELYRIVNPFGRYGIDGEAVFSYGQNSGTDAVFVTSTIKELELQIEQAVDLLKRPD"
     gene            13696..14253
                     /locus_tag="PL000_15195"
     CDS             13696..14253
                     /locus_tag="PL000_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90284.1"
                     /translation="MKKKILPVILTIALILTSCLKVKGAEAEPMTNVSESLALDLAFS
                     YSKDTRLGGWLVPPEKAYGQVMTYDEAMLFVFGKSVEPNTKEYEKRNKTIWLIVLEGE
                     FVEHVPASAGGDIPATEIIHKQMIIFLDGETGEIMREILPSPQMELPVTNLPVLQKTN
                     EEFPVLPTRAPALTEVPYPTLPAIQ"
     gene            14401..15198
                     /locus_tag="PL000_15200"
     CDS             14401..15198
                     /locus_tag="PL000_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90285.1"
                     /translation="MKNKRQYLKIFLLIAVVGIAFGFFVKAGAQVDVVTYLIEEFKLQ
                     SVPVKQVVVLEENPLKIQVVAVSFQNEGITFEDHLTLNSVDRIVALSAREKGYSIQSY
                     RTIIQDNKGNQLFGEERIIDVEQINSVLNRIQTNISDNSVNTLAIREVEMLLSDYKLE
                     NVSAQVDVTSLNGSRYLYIQLSLPSMDNAEKAATLLWVFHHTKYFRELEENGSQIAVY
                     RAKLLDEKGVTLMDYLYDYEINSGSWVQDERIPPLGGSPGADPAASP"
     gene            15224..15847
                     /locus_tag="PL000_15205"
     CDS             15224..15847
                     /locus_tag="PL000_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90286.1"
                     /translation="MKKTLMVVICVIFGVLYFSIPPSVAVQSRPDYHIPDTPEAKEVI
                     KAVERAYDVQAEAKFNFDIKKFPDIFINDPRFPVSSATLKTIRELTNNPSVESAGYLD
                     YKVAYYSWLIESTLHAEKVKEKAKQENRPLTAEEKKSLVDQNGRIAPVRSESPKRTLS
                     LTFLSVSVDEDVATVVLNDGPWTLEMYLVLVDKKWYVAGTKGLVFHP"
     gene            15976..16446
                     /locus_tag="PL000_15210"
     CDS             15976..16446
                     /locus_tag="PL000_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC90287.1"
                     /translation="MESTNEELRELTLLEQIESDPDVNQSTLATQLGVAVGTVNWHLK
                     RLIAKGAVKVSRAERKKLRYIITPEGIALRARLAVNYVEQSFSVYRRTRQKVKDHVTK
                     IRHAGYDRVRIVGSGDVADICKLTCLEQGIAVVNDKSAPALILDGYKVRLEGLE"
     gene            16443..17453
                     /locus_tag="PL000_15215"
     CDS             16443..17453
                     /locus_tag="PL000_15215"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC90288.1"
                     /translation="MTDRHVLITGGAGYIGSILTSELLRQNYRVSILDSLLFGGESIV
                     PFLSHPNFHFVKTDVTEKRAVKDAIKKDWQKPDTVIHLAGIVGFPACQAVGKQVAWKY
                     NVDATKMVFEQSADLGVGRFVFASTYSNYGLSPDGKPVTEETPLNPQSLYAETKIAAE
                     EFLLAQKDAVIAPLLFRFATLYGISPRTRFDLIVNQFVLDAFTKRSLIIYQRGYSRSF
                     VHIRDVVRGVIMGIEAETSKIRGQVFNLGTENGNYSKDDIVRLVLKRMPETVVEYKDL
                     TFGGDMRDITVSFEKIRTVLGFGTTLDVDDGVREVLFALKSGLIRNPIDDRYRNAQFI
                     VQ"
BASE COUNT         3896 a         4217 c         4452 g         4867 t
ORIGIN      
        1 acggcgtatc agggcattta caaacttccg cccgcgcaca ccctcgtttg ggaaaacggc
       61 gcggcacgca ttcaaaaata ttgggactac aactatctcc ccaaacacac tgccagcgaa
      121 gatgaactca tcgaagaact gcgcgcccgc ctgcgtgacg cggtcaagat gcgcctgctc
      181 agtgaacgtc cgcttggggc tcatctcagc ggcggcatcg actcgagcat catcgtcgcg
      241 ctcatgtccg agttagccag cggacctgtc aaaacgtatt cggctggttt cgaggaacag
      301 aatttcaccg agcttcccta cgcccgcgcc gtcgcgcaaa aatactccac cgaccatcac
      361 gagttcacgc tgacctacgg cgacatcccc gccacgcttg aaaagatcgc ctatcacttc
      421 ggcgagccgt ttgccgatgc ctctgccatt ccgctctatc acctctccgt gatgactcgc
      481 gagcatgtga ctgttgcgtt aaatggggat ggcggcgatg aggacttcgc gggctatcaa
      541 cgctactggc tggatggatt cgccaacgcc tacgcccgcg cgcctcgttt cttaacccgc
      601 agcctgatcc cgtccattgc aaattttctc cccgataacg cggaccgccc cgtggggcag
      661 agtctcgtca acggcatcaa acgcctcgaa cagattcccg aaattgaccg ccgcgccagc
      721 atcctgcgct ggggatcgta tttctcgccg cgtcaacgct ccgccttgtg gaaacctgaa
      781 ttccgatttg attctgccaa cgcccaaaat ttactggcaa agcaatttga ctctgctcaa
      841 ggctcctacc tcgacaagac tctctacacc gacctgcata cctacctccc tggcgacttg
      901 ctcgtcaaag cagaccgcat gacgatggca gcctctctcg aaggacgttc acccttcctc
      961 gaccatgaaa tcgtggagtg ggctgcccgt ttgcccgacc agttcaaggt caaaggaaga
     1021 agcggaaaat atctgcttaa gaaggcattt gccaaagagt tgccnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnntcaaag gaagaagcgg aaaatatctg cttaagaagg
     1201 catttgccaa agagttgccc gatgccatta agggccgcgg caaacaagga ttcggaatcc
     1261 ccgtcagcgc ctggttccgc ggaccgctgt atgattggtc gaagcagatg ttattggcgc
     1321 agggcagttc attgcatcaa tggttcgatg caggtgcgtt ggcgagactc atcgatgaac
     1381 acaaatccgc gcgcgtcgat cacggcaagc gtctgtatgc gctgtccatg cttgcggtgt
     1441 gggcaaactc gttatagcat gaccgattcc ccaaaaaaat tcccgctctg gaacattgcc
     1501 aagatcatcc ttgcgttgat tttggtctgg ttcgtgttat ccaaaacaga cctagcccaa
     1561 ctctacgccc tgcgtgaacg gatctccctg ccgtggctcg cgctgactct cctcctgtat
     1621 ttttccctga ccctactcaa agccctgcaa tattattttc tcattggccg ccgcgtggat
     1681 tatccacagg tgttgaatat cgtcgtggtg caaaatgcca tttcgaattt catcgccaca
     1741 agcgcgggca ttgcatcgta ccttgctttg tttcgcgtcg agcagggagt caaggtcagc
     1801 cgcgctgcgc tggcttttct cctcgccaaa gttggagatt tgatctccat ctggctgttc
     1861 atgttggttt ccagtttgct cgtctggccg ttggtggctt catttcacag ccttatcctt
     1921 ctttccttgt cttttattgg tgtggcaatt ctcggttttt ttgcagcagt ttttctgcgc
     1981 cagaaatttg tttccgttct ggcctggttt gtggaaaaac tcaaactgtc gcggctcggc
     2041 ttcgtcaata aactcatgga tctgcttgcc agcctcgccg aacaggagca tggatttgtt
     2101 tttcgcatgg ttggcacggg catcgctttc tcactgatct acatgaccgt caccatgctg
     2161 tggttgtacg ccagtctgca aaccttttcc ttcgaaattc agtttttaca agttgtcttt
     2221 gttaatatct tcatgcaatt gatttcctac ctgcccattc aagtcttcgg tgggttgggc
     2281 gtcaacgaaa ccacatcgct gtatctctat agtgttttca acttcccgca gacggaactt
     2341 gccgcgaccc tgatcggcac acgcctgctt ttctatctga caaaccttgt ggtgctgtta
     2401 tacttgcccg tgcatacttt gttttttagc cgctctgcgc aaaaacaata gcatatcttc
     2461 aatcgtttgt ccgtttctcg atccgcttta tgcaaaatgc ctcccctaaa aacatcattc
     2521 aattactcac tgtgggtctg gtttcaggcc tcgtttttgc tttcctcgaa atcaaggctc
     2581 tgtggggccc cgatcaaatt tggaataaat tactcacgcc actcggcgta gccgcaatca
     2641 ttgcctacat tttgcttgcc cttgttgggg tcttcatcct ctttttgagc ttgtggaatc
     2701 cgtcgccgct tcgcgcattg gctcaaaaaa taaaaagcct gcgctggctg gtcgcatttg
     2761 ccacaatcgg attccttgcc tggatgtatc tcttctctcc gtggcaggcg accctcacca
     2821 taccgtggac tcaattcatc gtggctgcgg ctcttgcccg actgttggcc tggtgcttcg
     2881 tctccgaaca ggatcagccc ttcggttgga gtgaccttgc actggcgttc agcatttttc
     2941 tgtatccgcg catcgtgcag gaaatacgtc aatatcttcc tgcgccgctg ctcacccgtg
     3001 gatcgattgc ggtgggattt gtctttcaat ttgctttgct tgccgctttg tatgaatcag
     3061 tagggaagtc cctgctcgca aaattgatcg agcttcgagc ccgcctcgaa cctgttcgtc
     3121 taattttggc tgctttgttt ttgctggctc catttattta tcgttattcc actggcccga
     3181 aagaatacat tttatatttc aatatccgct tcatggttgc cttgatcgct ttatggtttg
     3241 ccgcatatct gctttgcaaa gactcaagcc gactcatttc cccagagtct tttggcgttg
     3301 cggctggctg gctggtgctt gtctctgcca ttacgcgctc actcctgctt gttgtggatg
     3361 atcccttcgg cctgtactgg tctgaaggca accgcttgta cgattattcc cttgttttcg
     3421 ggcaagattt atataactac gcggggcgaa ttcccgatcc ctacaacacg cccggacgtt
     3481 acggtttgtg gggcgtgctc tacctctggc agggacttcc catctgggcg catcgtttgt
     3541 ggaatgttgc cttgcttacg gtcccatctt ttgtgctggc gtgggcattg accaaaaaac
     3601 tgtccccgtc ctttttacgg cagatggttt ttctttgggt cacagctttc tttattttgc
     3661 ttgcccctct gcatccgccg ttcatgatta tgacggtcat tgtggcattg ttcattttcc
     3721 acccgtcgcc atatgtgcgc ggtgcttcgc ttgtggctgc cagtctttac gctggcatca
     3781 gccgctggac gtgggtcttc gcgccaggcg cttggggtgc gttgaccgac ctgcttttgt
     3841 tttacccaaa gcgtgaaggc aattggttca agcgtttgct tcccacggtt ttgatggccg
     3901 tgctcggcgc tgttcctggg tttttgctga acattgggaa ttttcttgga tatacaacgg
     3961 gagagaatac caccgcgcag cagccattgc tttggtatcg cctccttccc aatctgacac
     4021 ttggtccggg tatcgtattg ttgatcctgc tgacgacagg tccgctcttg gtgttgcttg
     4081 cttacaaaat gtccacacgc caatggaaat tggatggatt tcagttgctg gctgtgtggg
     4141 gggcgttgat cggtttcttc acgattggct tggtgatcag caccaagatc ggcggcggcg
     4201 gtgacttgca caacctcgac atgtacatcg gcactctcat cgtggtgacc atgcttgggt
     4261 tgactgggca ggttgagggt caggatgtga agcgctggtc ttcatccgca ttgacgatgg
     4321 tatgcctgct tgccattctg ccgatctatc cattcacacc atttcatcca tcggctggct
     4381 cgcatgcctg gctcgacctg cccacccgcg aagataccga caaggcgttg aaggtcatca
     4441 acgattcggt ggcgaattat tcgcaggcgg gcgaaatcct tttcatggat caacgtcagt
     4501 tgttgacctt tggctacgtg accgcgatcc cgtttgtgcc tgagtatgaa aagaaatata
     4561 tgatggatca ggcgctggct tcgaatgaga catacttccg cccgtattat caggatcttg
     4621 caaataaacg ctttgcgttg atcgtaaccg agccgctcaa agtggatttg aaggaagagg
     4681 gcggtgtgtt ctctgaggaa aatgacctgt gggtcaagtg ggtttccgcg ccgaccctgt
     4741 gtttttacga gcaagtccat gccgataaga cggtggggat tgcgctgctt gtgccgcgtg
     4801 agaatcctgt tggctgtgaa aaatatttga aataataaaa tcggtggttg agcatgccca
     4861 aacccctgca agggccacca ctaaaaaggt actctcttga atttatctta tcttcttcct
     4921 cgtatacttc gccacttcat gcctgagtcg gtggcgcgct ttttattgaa acgccgctgg
     4981 atcatccgcc ctgggttgga atcaagcgat ccatttgccg cagtggaaca gtatatgtct
     5041 ggggtaaccg ccagcggaaa atccctgatc ggaaaacggg tgctcgtctt tggttatggc
     5101 ggtcgtttcg ccgtgggtgt ggaattgctg cggcgtggtg cggcgcatgt ggtgttgtgc
     5161 gatcattttg tcctgctcga caaggagcgt aacctggagt tgctccctgt ttaccctgag
     5221 tatcttgtgg gcgaaaatga taatgtgcgc ccgcgtgcgg aatatatcac cctgttgcat
     5281 ggggatattc gcgaggatgc aattcagaag cagatcacgg aagtggatgt tgtcctcagt
     5341 acatcggtct acgagcattt ggacgatgtc aaaggcatca cccatgccct cgcgaaattg
     5401 accgcatcca atggattcca cctgcatttt gtagacctgc gcgatcactt tttcaaatat
     5461 ccctttgaga tgcttacttt ttccgaaggt gtgtggcgga atttcctcaa cccgacgagc
     5521 aacctgaatc ggtttcgctt gggcaattat cgggaagtgt ttgatgcgta ttttcgaaaa
     5581 gtggaaatca agatattgga acgtctcgaa cctgaatttc gtgccatccg tgcgcgcatc
     5641 cgccctgagt tcctgacggg tgatgaggcg gttgattctg tcggcttgat ccaggtcatc
     5701 gctgagatga gaaagtaaag ccgacttaag tcggcgctgt gccagttgga gattttttta
     5761 tcatgcaaca aagattttcc agttttatca tccgaaacga aaagctcatt cccgttttta
     5821 tctttctgct ttttctcgcg gcatcccttc caggtgtgga ttggggtgtg cccgcgcttt
     5881 ggaatcccga tgaaatgatc tggcgcgtgg atagcgcctt gcgcggcgag atcatcttcg
     5941 atgagaccga acccgatttc aattacccgt cgctccccaa atatgtgatg ttcggcatcg
     6001 gcaagatcgt gtatgggttg ggcggcacgc cttcagattt ttacatttcg gcacgttcgt
     6061 tctcggcatt gctgggagcg gtggctggtg tgcttgttta tttcatggca cgcagcatcg
     6121 gtgtggatgt gttgacctct gcgttggctg gcttgctgta tgttgccagc ggtgtagccg
     6181 cagcaaatgg acgctttgca cacaatgacc tgtatttgca gttgttttcc gtcctctgcg
     6241 tttatttcgt gatcaaatat caatttacaa aagcgcggct gtggttgtat ggttcattct
     6301 tttcggtggg gttggcggct tcgagtaaat ttacgggcgg cagcttgctg cttgtgccat
     6361 tgttcgtttt tgttttcatg aactggagcg aggttcgcag gaactggctg caatcgttcg
     6421 agatgctttt cattggcggc gcgctcagtt tcggcggata cattcttggc acgcccaagg
     6481 ccttgctctg gatggcgtat tatttcaagc gcgtcattcc cgcgttgcag cgttatccgc
     6541 aatatggttt caactcaggc acgcccatcg gcttgtttgg tcaatggccg gtgttcgaag
     6601 atgcagtggg gaccttcgct tactatattt ttattctttg ctttatctgg tttgtagcca
     6661 aaataattct ttggaaactc ggcaggctgc aaatggatga aacacaggca caggcgatca
     6721 tgatccttgc cgccacggtc ctgctgttcg acctgccgtt catgatctcg atcaattaca
     6781 tcccgcgcta tttcatcccg tttgtgccgt tcctctccat tttgggcgcg cttttcatca
     6841 aagagatcgt tgaaattgca aaagccaaaa actggaaata tgttccgcag atcgtttccg
     6901 tcttgctggt tgtgggcatt acctattcca tgctgcggct ggcaagcacg gccctgctct
     6961 tcatgaacga tgcgcgcatg cccgccagcg aattcgtcgc aaacctgcct ggcgaaggca
     7021 agaccatcga atacaccttg tacccgccca ttgtgaacaa aaagcaattt gcaaaagcct
     7081 ataactatcc cgtctttttt ttgaaatacc cgactgacgt tgtccccaca ggcggacgat
     7141 acgaatacaa tcaaggcgag gcgggattaa tagaccgcga aaccgactac ctgattattg
     7201 atacgcttac ctatgggcga ctctacaccg aatccgtgtg cgtgaccaac cccgtggaat
     7261 gtgatttctt caagcgtctg gttgcgggcg aaataaagac cttccgcctg atgaaggaat
     7321 ttacctacac cctcccgccc tggctgccag atatctctct ttcagcagtc aaccccgagg
     7381 tccgcattta tgagcgggtg cgttaaaaag agacagttac cttcgtgtag cgggtgactg
     7441 tctctttttt ttttatttca caaaattccc caccgtatac acagaccctg caatcggcgg
     7501 cgtgctctgc atgtggatga catgtccctt tgaaaacgcc ttgtgcgcgg cctcttcgat
     7561 ttccttctcg gtcgaaaaat gttcatcgcg gatattatgc ccgagcaagc tgaacatgaa
     7621 caccatgaac ggtttttcaa cgggagttcc atagaccatt tgcccgccag gtttcagtac
     7681 gcggttcact tccgcgaagg cctgcttcaa ttcggcaggt ttgagatgct ccaaaatgct
     7741 gaccagcaaa accgtgtcga agaagttgtc ctgatacggc atcttcaaca cgtcgccctt
     7801 ttccagaaac agcggaattc ccgtcggctc aaaaacagac ttgacggcat ggatgtctgc
     7861 ggtcagatca atgccatgga tctccttgta catgtcgtgc aggttgggga aagccagtcc
     7921 cgtgccgaag cctacttcca acacgcgctc gccgcccgtg cattcaccca atgccaattc
     7981 cacgcgttga cggtacatct tcccgaaaac gggccagtaa tagtatttga tcgggtcgta
     8041 ctgattgacg cctttgtatt gtgaggcagg gagtagtttg agtttcgggg gcatgctttt
     8101 tcctgttttt tgttttaagt cccgtgattt tacctgatta aacaaactac ggcttcccat
     8161 ctctcggcgt tgttttgctc tttgaggata tgcgccaatg gcgtataatg gttttgatga
     8221 ttgttgtaga aacacatatt ttcacgcggc aagttttgtt gcttttgagc gatgaagagt
     8281 accgtttatt gcaggcagta cttgtcaatc gtccggagtc tggtgtgata atcccttcga
     8341 gtggcggatt acgaaagatg cgctggggat ttttagggaa gggcaagcgc ggcggtgtac
     8401 gtgtgatcta ctattgggca gtcaggcagg aacgtatttt gatgcttttg atttacccca
     8461 aaaacgtgaa ggatgatctc acgcgggaac aattgaaaat tctgcggaac attgtcaaag
     8521 aggaattccc atgaaagaag atttgtttaa tgagctggtg gtaagtgtgc gcgagggtgg
     8581 cgcaattttg cgcggagaaa aaaaacctgc acgtgcattt aaaataactg ccccaaatat
     8641 caaacgcatt cgggcaggtt ataaactttc ccaacaagaa tttgccgctc tactggggat
     8701 tagtgttgcc accttgcgga attgggaaca gggcaggcgg atgcctgaag gccctgcacg
     8761 ggttttgctt caagttgccg cacgctatcc tgacgtggtt tgggatgtgg tgaaacctat
     8821 ccgatagcca atctcattta tttggatttt gttgttatgc ccttttcctc ttctaaaaaa
     8881 tggagcgccc tcttcgctgt gtggattctg catggcgttg ttgccctgtg gcaatttttg
     8941 tccctgccat cggaggggct ttcgcttcaa cgcattgtaa tgcttgggct gctgctggcc
     9001 tgggttgcct tcgccatgtt cctggctttc tcggcttttc gccagctcca ctggttgaat
     9061 ggacttctac tccgaatcaa taaaccattc ataagagatg gtatttttat cgctgctgtt
     9121 ctggctttgt ttgtccgcat cagcctgatg tttttgctcg ggctggccgc ccagactcca
     9181 gagtttcatt acggcgccta tgttgaacgc ctgtcgccgt ttttagacct gctggctttt
     9241 gtcgcctttg agatcatcgt gctgaatttg ttctttgtct ttcgggaggg gacagaatat
     9301 ttcggcttga tcaagtccat ttcggcgaaa ctgttcatcg gtctggtttt gatcgggggt
     9361 gtggtccttt acatttcgaa aaccgacatg ggcatcgctc ccatttacaa aggcgactgg
     9421 gcgcgtggac ttcccgccgt gcccttgctt gaatggcaga tttttcttgc cgtgttgttt
     9481 tgcatcggta tgttcctcgc cgaagtggat ggacgcctgc tgaaaattcc cagcctcgat
     9541 ctgtggattg ctcttgcggt ttgggttgcc acggtcggat tgtggctgag ccagcccact
     9601 gtgccgaatg cctcggctct cgaaccgcgc gaaccgaatt acgaaatcta tccattcatt
     9661 gacgcgcaca cctacgacaa attcgcccaa tccgttttga tcggcaacgg gtttggcccc
     9721 aacgaaattc cgcagcgccc gctgtatatt gtttttctgg ttttcatgca cacggttgtc
     9781 gggcagaatt acgaaaatgt cattgcgctc cagaccgtgt tctttgcagc gttccctgtt
     9841 gtattgtata tttttggcag ggaatttttt gggcggccca tcggcgtttc gctggctttg
     9901 ctggctgcct tgcgcgattt cacctcaaat ctggtttcgc ccatcacggg gaatatcagt
     9961 tattcaaaac tgtatctctc cgaaatcccg actgcgatct gtctgatcct gttcttgctc
    10021 atcggcatcc gttggatcag atcgggtttc ccgctttatc tcggttttct gcttggcggt
    10081 gtattgggta ttggcatgtt gaatcgtacg caagtggttg ttgctctgcc tgttttgctt
    10141 ttctttgcgt tcattcttca gccgaaaaaa cttctcttca tcattaaggg cacgcttcca
    10201 atgttggcag tgatcgcgct ggtcgttgcc ccgtggctgt ggcgcaactg gcagatgacg
    10261 ggcaagatca tcttcgataa tcccgcctcg caaaccgcca acctcgccct gcggtacaac
    10321 cgcctcaacg gcgaagatgt ggatattctg caaaagcctg gcgaatctaa ttctgattac
    10381 aacgaccgca tggttgcgat ggcgaatcat gccatgtcgg tgaactcgca gggcattttc
    10441 aaggcgatcg caagttcgtt tatcaatcac ggcatcaaca acattttgct tttcccgctc
    10501 cgcaacgacc tcgcaactcc cgctgatctt attaccccaa ccacaccatt ctggcagcaa
    10561 tgggaaggcc gtcccaactt cctgcaatcg ctcttgctgg ctttttacgt ttttcttttc
    10621 tcgctcggtg tcaccatggc ctgccagcgc aacggctggc ttggccttct gccgctcatg
    10681 gtcaacctcg cttacaacct gtggacctcc atcgccctgc tttctggtca acgcttcatg
    10741 ctttcgatgg attggtccat tgccatgtat gatctgctgg gcattttcgc attgctcagt
    10801 atctttttct atgccctcga aaacggacgc gcgaaaattc tcaaatggta tgaagccaac
    10861 acggtttcca tcattccaac cctcgaagct ataaaatggc cgcgctatgt ttttgcaggc
    10921 ctgatgtttt tcgcggtggg tgcatccctg cctttgagtg agatgtcttt ctcacagaga
    10981 tacccagccc ttgagcagaa tcaacccgct gacctgactc tcctctcaag cctcgctcaa
    11041 accgacctcg aacctgcctg ccaaaaactg gcagaatcca gcgggttgga gatcgtgcgc
    11101 gggcgggcgt tgtatccgcg tttttattca gcaggtgacg gtgagatctt cactgattcg
    11161 gttggctaca aaaaagtgga tgaaggcagg ttggtgtttg acctgctcgg ccagaagaat
    11221 caccgtgtca tcttccccat gagccaatcg cctgaatttt ttcccaatgc ctccgatgtg
    11281 accatctggt tcgataaaaa cgcatctccc tggttcattt tggttgaaca gggagatgcg
    11341 caaaggtttt atgtttcaga tagggtgtgt aagtaatctt ttggtagcgt ggactttagt
    11401 ccgcatttcc aaagcacaga ctgaagtctg cgttacgcga gttacacaaa cttaattcgt
    11461 ctcgccgcaa aggccaccat tctaaacaac agaatgccgt gcttccagcg cgagatgttg
    11521 gtcgtgccgt aggtgcggtc acgatagcgg atggggagat cgatgatctt gcggttgagt
    11581 ttggccgcgc caaagatcaa gtcaaaatcg ccaaaggggt cgaaatcgcc aaagtaggaa
    11641 cggttggcgg cgatcttttc gtaatcttca cgccacatca cttttgtgcc gcatagtgtg
    11701 tctttgatcg gttgtcccaa cagccacgag aatgccatgc tgaataattt gttgccgatg
    11761 aagttcagcg tgcgcatggc ttccttttcc attggataca ccagacgaac tccgttgatg
    11821 aattcgccct tgcccgatgc gatggcgtca tagaatcgcg gcaggtcttc gggcgggacg
    11881 gtcaagtctg cgtcgaggat catgaggatg tcgccggagg ctttctcgaa gccgaggcgc
    11941 actgcgtcgg ctttgccaat gcctgtttgt tgaaagagca ggctcggggt tgaagggtga
    12001 agcggaattt catcctgaat tgtctggaag gtgttatcgc gcgaatgtcc ttcgacgaag
    12061 atcaattcgg tttttgatcc catcctgggc agacgctcga aaatgttttt gatgttgcct
    12121 gcttcgttac gtgcggcgac gatcacggag acgcttgggt ttttcacagg cgtggctgca
    12181 gggcgcgcaa cgacaaaatt ggacagcgcg aaatcgcgga acggccagag tttgacgagg
    12241 taacgatctg cgaggccgcc gaggggcaga ggccagagca cctcgcgcat gggacggatg
    12301 ctctcgaagc ccgcgaggcg cagcatgttg tccacgtctt cgggggtgag ccagttttgg
    12361 ttgagcatgg gcgcggcgag gttgagacct tgcgcgattg tcagcggaag ctgccagagg
    12421 tggctgtaaa aattgaggat caggcgcgtg tgcggcgtgc agaatttttt cacctgttcg
    12481 agcgcgcgct gtacatccca aaggtcgttg accgaatccg aaaagatgac cacatcgaag
    12541 gtgccttcaa ggctggagag gtcgtgggca tcgagctggt gatattcgat ctctgggtgg
    12601 cgggctttgg cgcgcgcgat catttcgggt gagaaatcca cgccgacgcc gtgcgagggt
    12661 tgcagatgcg cgattaagct gcccatgccg cagccgatct caaggatgcg ctggttgggg
    12721 ttgacaaggt atttgtagat ctggatcaag cggcggtgat accagcgccc aagcccggcc
    12781 caggcatcgc gtttcttcgc gaccttgtcc cagtgggcga tgcgggtctt ctggtattcc
    12841 atgcctgctt catcgaaaag tggagttgtt ttcatggcgg ggtgattgta ttcgcggtgg
    12901 ggcagtttgc aaaggtggaa aattttgtag atatgaattg tcttttgaaa atcgtccgct
    12961 tgcacgaaat gaaacacgtc aatataatcc attgtatggg gtgaattgat tttgttcccc
    13021 caaccaaaga aaggttgcaa attatgaaaa aaacgatcct tgcgatcttg acgcttgcct
    13081 tggttttgac ttcatgtgca aaagcgaaag ataccgaagt agcacctgtt tttacaggcg
    13141 ggagctctac tgtcgaatgg gcttccaaag cggcaactgt ggaagaactt gtgtcagagg
    13201 cagaccttat cgtgagagta cgggttattg aggcgcctgt tactcgagtg cttcgtcatg
    13261 aacttccggt ttggggtgaa aataataaaa tagttggttc agaaacgttc gaagcgcttt
    13321 tctcggacac agtctttgaa gtagtaaaga cgtatctagg tgatccacca tcgcaaatca
    13381 ctgtcatgca aattggcggg tatgacccaa ctgtgtcaaa gggaattgaa gaggttggag
    13441 atgatcctct gtacaaagta agagaggggt atatcttgtt tttagtgaac atctctggtg
    13501 accaaattca cgctccagat cgtgaacttt atcgaatcgt aaacccattt ggacgctatg
    13561 gaattgacgg tgaagcggtt ttttcgtacg gtcaaaactc tggaacggat gcggtttttg
    13621 taacttcaac gatcaaagaa cttgagctgc aaattgaaca agccgttgat ttgctgaaaa
    13681 ggcccgattg aagatatgaa aaagaaaatc ctgcctgtaa ttttgacaat tgctctgatt
    13741 ttgacatcct gcttaaaagt gaaaggtgcc gaggcagaac cgatgactaa tgtttcagag
    13801 agtcttgcat tagaccttgc gttttcttat tctaaagata ctcgtttggg tggctggttg
    13861 gtgccgccag agaaagccta tggtcaagtg atgacctatg acgaagccat gctgtttgtt
    13921 ttcgggaaat ctgttgagcc aaacacaaaa gaatacgaga agcggaataa aaccatatgg
    13981 ttgatcgtgc ttgaagggga gtttgtagag catgtacccg cgtcggcagg tggtgacatt
    14041 ccagcaacgg agattattca taagcaaatg atcatatttc tagatggcga aactggagaa
    14101 atcatgcgag agatcctgcc ctccccacaa atggaattgc ctgtaaccaa tttgccagtt
    14161 ttgcagaaga caaacgagga atttcctgtg ttgcccacac gagccccggc tttaacggaa
    14221 gtgccttacc caactttgcc agcaattcaa tagctgtaag ggatgtcaat ggtaattggc
    14281 ttttgttcga tcaaccttcg tgggttgaag attacccata tcttatttgg aataaatctc
    14341 aaaattcttc atttcgcacc tacgggcctg tgccgtagta gaagttgatt ggaggtaggc
    14401 atgaaaaaca aaagacagta tttgaagatt tttttactga tagctgttgt gggaatagcg
    14461 tttggctttt ttgtaaaggc tggcgctcag gtagatgttg ttacgtactt gattgaggaa
    14521 tttaagctgc aaagcgttcc ggttaagcaa gttgttgttt tagaagagaa ccctttgaaa
    14581 attcaagttg ttgcagtcag cttccaaaac gaaggaataa cctttgagga tcatcttact
    14641 cttaactcag tagatagaat agtcgccctt tctgcccgtg agaaaggtta ttctattcag
    14701 agttacagaa cgatcattca agataataaa ggaaatcaac tttttggaga agaaagaatt
    14761 atcgatgtag aacagataaa ttctgtactg aatagaattc agacaaatat atccgataat
    14821 tcagttaata cactggcgat tagagaagta gaaatgttgc tcagtgatta taaattagaa
    14881 aatgtatcag ctcaggtaga tgtaacttcc ttaaatggat cgcgatattt gtatattcag
    14941 ctatctttac cgtctatgga taatgctgaa aaagctgcta ctttgttatg ggtgtttcat
    15001 catacaaagt attttagaga acttgaagaa aatggttctc aaattgctgt gtatcgggcg
    15061 aaactgctcg atgaaaaggg agttacatta atggattatt tgtatgatta tgaaattaat
    15121 tctggtagtt gggtgcagga tgaaagaatt cctccattgg gaggaagccc tggcgcagac
    15181 ccggcagcgt caccataatt atggacctta attagaggca aaaatgaaaa aaacattgat
    15241 ggttgttatt tgtgttattt ttggagtgtt gtatttctct attcccccat ctgtggctgt
    15301 tcagtctagg ccagattatc atattcctga tacgccagaa gcgaaagaag ttattaaagc
    15361 tgttgagaga gcctatgacg ttcaagcaga agctaaattc aactttgata ttaagaaatt
    15421 tcctgatatt ttcataaatg acccgcgttt tcctgtaagt tcagcgactt tgaaaacgat
    15481 cagggagttg accaataatc cgtcagtgga atctgcagga tacttagatt acaaggtggc
    15541 atattattca tggttaatag agtctactct tcatgctgaa aaggtaaaag aaaaggcgaa
    15601 gcaggaaaat cgtcctttga cagcggaaga aaaaaaatca cttgttgatc agaatgggcg
    15661 cattgcgcct gtgcgttctg aaagcccaaa aagaacactg tctttaacat ttctgtctgt
    15721 gagtgtagat gaggatgttg ctactgtggt tctaaacgac ggtccctgga cattggagat
    15781 gtatcttgtt ttggttgata agaaatggta tgtagctgga accaaagggc ttgtttttca
    15841 tccataaact ttagggtata gcaaagaagc cacgtgattt gtccaagtca cgtggcttct
    15901 tgttttccag aatcttctct accccgtctc aaatgggtta taattcagcg actgaatttt
    15961 ggaatgaaat ttgagatgga atcaacaaac gaagaactcc gcgaattaac cctgctcgaa
    16021 cagatcgaga gcgatcccga tgtcaaccag tccacgcttg ccacgcagtt gggcgtggcg
    16081 gtgggcacgg tcaactggca tttaaagcgg ctcatcgcca aaggcgcggt caaggtctca
    16141 cgcgccgagc gcaagaaact gcgctacatc atcacccccg aaggcattgc cctgcgtgcc
    16201 cgccttgctg tgaactacgt cgagcaatct ttttccgtgt atcgccgcac acgccaaaag
    16261 gtaaaagacc atgtgacaaa gatccgccac gcaggatatg accgcgtgcg catcgttggc
    16321 tcgggtgatg tggctgatat ttgcaaactc acctgcctcg aacagggcat tgcggttgtg
    16381 aatgacaagt ctgcgcctgc gcttattttg gatggataca aggttagatt ggagggattg
    16441 gaatgactga ccgtcatgtg ttgatcacag ggggcgcggg atatatcggt tccatcctga
    16501 cctcggagct gctgcgccaa aattacagag tcagcatcct ggatagttta ctgttcggtg
    16561 gcgagagcat tgtcccattt ttgagccatc ccaactttca ctttgtcaaa acagatgtga
    16621 ccgaaaaacg cgccgtcaag gatgcgatca agaaagactg gcagaaaccc gatacggtca
    16681 tccatctcgc gggcatcgtt ggttttcctg cctgtcaggc ggtggggaag caggtggcgt
    16741 ggaagtacaa tgtcgatgcc acgaaaatgg tcttcgagca gtcagcagat ttgggcgtgg
    16801 gacgattcgt attcgcatcc acgtatagca actatgggct gagccccgat ggaaagccag
    16861 tcacagaaga gaccccgcta aatccgcaat cgttgtacgc cgagaccaag atcgccgcag
    16921 aagaatttct gcttgcgcaa aaggatgcag tcattgcgcc gctattgttc cgctttgcca
    16981 ctttgtatgg aatttccccg cgcacaagat ttgacttgat cgtcaatcag ttcgtgttgg
    17041 atgcatttac aaaacgctcg cttatcatct accaacgcgg atactcacgc tcatttgtcc
    17101 atatccgtga tgtagtgcgc ggcgtgatca tgggcattga agccgaaaca tcgaaaatcc
    17161 gtggacaggt tttcaacttg gggaccgaga atgggaatta ctccaaagac gatattgtgc
    17221 gcctcgtttt gaagcgcatg cccgaaaccg tggtggaata caaagacctg accttcggcg
    17281 gcgacatgcg cgatattacc gtgtcttttg aaaagatcag aactgtgctt ggctttggaa
    17341 caactctgga tgtggatgat ggtgtgcgag aagtattgtt tgcgctgaag tctggcttaa
    17401 tccgcaaccc gatagatgac cgatatagga atgctcaatt cattgttcaa taaatggaga
    17461 taaacatggc agagaatttc tggcaaaaca aaaaagtgat cgtcactggc ggagcgggct
    17521 ttttgggctc gt
//
LOCUS       DAOQLC010000094        17377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1162_length_17377_cov_21.691029, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000094 DAOQLC010000000
VERSION     DAOQLC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_17377_cov_21.691029"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PL000_15220"
     CDS             complement(<1..543)
                     /locus_tag="PL000_15220"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC90289.1"
                     /translation="MSQKNIKTISNPKLKLEVLTKEDVLKIHNATLQIIEKVGVRFPS
                     KRALEIWESVGAEVDHEKKIVRVKPQVIEDALKKAPPAYKLAARDPQQDLQLDGNHVN
                     LGTDGCGVEVIDIHTGEKRTSCLQDVRDIARVADATEEVAFHWVAVSAQDAPVEARGL
                     HELKAVWENSTKHVQTESIYN"
     gene            complement(625..1281)
                     /locus_tag="PL000_15225"
     CDS             complement(625..1281)
                     /locus_tag="PL000_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNC90290.1"
                     /translation="MSEELYKEMAQSIIDGDSDISTELAKKAIELNMHPLDAITNGFV
                     VGVNYIGDQFGKGEAFLPELVMAGEAMKAAVAVLEPELLKLGSAREMMGKVVLATVEG
                     DIHEIGKTLVGTMLSASGFEVVDLGVDQPADKIIGKALEIDAQIIGMSALLTTTMVRQ
                     REVIEELDKEGLRPRIKVMVGGAPITRDWVTKIKADGYSEDAIGAVKIAKELVGKADA
                     "
     gene            complement(1294..2025)
                     /locus_tag="PL000_15230"
     CDS             complement(1294..2025)
                     /locus_tag="PL000_15230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC90291.1"
                     /translation="MEKELTHKPLKEEIYDALHRQIIAGKYTPGDWLRQEDIASQMGV
                     SMTPVREAFDLLVAKGIAERVPYRGVRVREISAREVVEAYGLRLMLEALIAREAAENI
                     SPQQLTQIETIMEEMDRHVILSEMPQERQLSREFHAAIAEASGNALMVQVYTIVSNAF
                     PDWLLYEALFRKPELVDESVRQTHDEHAAILDALKKHDADLAVKVSLEHVMESGRWLE
                     THLNIPAKLLKEKEKQITHLLKKPK"
     gene            2189..4075
                     /locus_tag="PL000_15235"
     CDS             2189..4075
                     /locus_tag="PL000_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNC90292.1"
                     /translation="MSDIDLNPLKPAIESYIPLGRSGLLPALHAAQKIYGWLPEEVAS
                     EVAKSLRVPLADVHGVIEFYSLYYNEQVGQKFIRVCTDPACALKGADGLLKHLCKNYK
                     VDAGQTTDDLSLTIEQSPCLGLCDQAPAALVDDDAETNISHNFRSYDIGVPQSLVYGS
                     IRELTANCGEGTTTLAKYGEYSAYKKALSVKPEDVIAEVKASGLVGRGGAAFPTGIKW
                     EGAAKASADQKYVICNADESEPGTFKDRVLLLDDPHRTIEGMCIAAYAIGATKGYIYI
                     RGEYPYIVPVLEEALNEARMAGYLGERFDIEIRVGAGAYICGEETALFESIEGKRGFP
                     RVKPPFPTTHGVFGKPTVINNVETMCNVPLIIEKGSAEYRKIGTEKSPGPKLFCVSGD
                     VSKPGVYEVPFGVTLRELLEMAGGVTGKKALQSVLFGGAAGAFATSEHLDVRMTFEDL
                     RAAGLPLGSGVVMVFDETRDMRDVLQRLGHFFAHESCGKCYPCQMGTQRQMEILNRVA
                     AGKTLPDDMIRLQDVGWTMTEASLCGLGQTAASAVLSAMKLWPALFENGGGKTKSDKK
                     SVAAKPRAASARKSVKAKAVKKTTVKKPVAKKTVKKAVKPSKPKAKKTATKKSTANKT
                     AKKR"
     gene            4086..4784
                     /locus_tag="PL000_15240"
     CDS             4086..4784
                     /locus_tag="PL000_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNC90293.1"
                     /translation="MTETVNLIIDGQEVSAEQGRTLLEVARAMGKNIPTICFHDATTA
                     NALCRICVVEVEGQRVLQPACIVKAGAGMKVQTRSEKVIRARRTILEMLASTMDLSEA
                     ADIQQMMVEYGASSERFPDAERRESEVKDDNPMYVRDYSKCLLCWRCVQVCAEDAQYT
                     FAINFDGRGFETQIGTFFDKAIPQTSCVMCGQCVGVCPTGALKPKREFLLEQGMSPSE
                     ISVMNTGRKQRKPR"
     gene            4781..6997
                     /gene="fdhF"
                     /locus_tag="PL000_15245"
     CDS             4781..6997
                     /gene="fdhF"
                     /locus_tag="PL000_15245"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNC90294.1"
                     /translation="MIKSDRVTTTTCPYCGVGCTLQLHVKDDFIYKVTSPFDSPVNQG
                     NLCVKGRFGYDFIYHPKRTTTPLVRRYLLEGKPKPAGREQVFAVSTDGKPIPDSKISN
                     DWDWVETDWDTALNFVADNLVRIYQRDGSDAMAVYCCAKATNEDNYLLQKMYRALFRT
                     NNVDHCTRLCHAGSVVALQQAVGSSAMSNTASQVIMNDVFIVTGSNTSENHPIIALQM
                     KEAVQKHGAKMIVIDPRRIDLVDMAEMWLPLKPGTNVPVFSAMAHVIVKENLVNHEFI
                     ANRTEGYQDFVESLEKFTPEYAEEISGVPAEEIRKAARMYAGAKNGAIYWGMGISQLS
                     HGTASAMALIHLAFLTGHIGRDGTGLNPLRGQNNVQGASDMGSMPFHYPGYMRVDNPE
                     NRAKWEKAWNIEEGGLSLKLGLTTTEILSHAHEGGVRALYIMGENPMMSEPNLNDTCR
                     HMMELEFLVAQDIFINESGSFADVFLPATPFAEKDGTFSNTDRRVQRVRAAQAPRGMA
                     RPDWQIICDVALRIESRLGRPTAKWDYTNPEQILREMGDVNSDYAGITYDRIDKIGLI
                     YPVPSMDHPGTPTLFKESFPRGRGKFHQLDYVPVREDVDDEYPFILTTGRLLEHWHGG
                     TMTRNSSLNEIYPEARVEIHPADAEIHGIRNDDPVKVQSRRGEVVVRATVTEKTTVGV
                     VFLPWHFHEAAANLLTNDALDPQAKIPEFKACAVQVFPARESDLPNPEIVLNRGRY"
     gene            complement(7066..10398)
                     /locus_tag="PL000_15250"
     CDS             complement(7066..10398)
                     /locus_tag="PL000_15250"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC90295.1"
                     /translation="MAKVFVSYSRKDTEFTKHLTQELQKSEIEFWVDWEGIPPTVDFM
                     KKIENGIEESDAFLFLLSPDSAKSKVCGDEIAHAVKNNKRLIPLMIRDVKEDEVPAAF
                     SHLNWIFFREQDDFDTSFKKLLQGINTDFEWVEAHKRLLIRALEWKNKNKDKSFLLRG
                     QDLHEAESQLALNSSKDPYPTDLHREYILKSRQATDQQRRWTTVFIAIAAVIMFALAV
                     YGLNSARLAVNEAARANESEQREATAKNLALLKETEAKINQLSALAMSKIDQSYNEAL
                     LLSVEAYLHAKDNKINDVNTQSAITKILQSHPGLHQILLGHEDWVNTMVLNPDQTILV
                     SGSWDYTVNIWNMSNPDFPVKLSTLKEFTGPVENVTFTPDGKILATSSDYELILWDIS
                     DPSSPTLLSKTDGYFTNLGFTVDGKILTSLNIHADVNNTIALFDVSNPKSPVELIQLE
                     SGDESLDINSAIFNRNKNILFFTRSDGSIVAWDLQDPNAPTVLSTTDGFGSKVTAIAL
                     SPDENTLALGDTNTLIVLWDVTDPAAPTIYSNWSGHSLSINSLAFTADGNTLASSSNE
                     TNSQIVLWDVTDRYVPYKIRTINGHSLGVNTVLFNANNNLLFSGSRDGSIMVWDSAQP
                     KTEMEIGAIDGTMIDMAIQPNSNILVSVDSDHKLTSWDISKLSSPVKIKTQDGPASII
                     LFNKDGSLMASMNFDETVTLWDMKDFSKLNTIKLSGETINAIEFAPDGKTLAINTEKA
                     SFWDVSDPKKPVKISTLSQESNEISDMTINADGNLLAGIAANNVILWDISDVTNTKRM
                     GVLEGHSNQVLAAAFSPINDSLLVTAGKDKTIILWNVDDPINPQKMDTLANHSDWISS
                     VAFSPDGTQLASGSYDKYVNLWNISNPEVSVLISKMVGHGGNIGTIDFDPSGVFLASL
                     GNNDNIIFWDITPASWVQKACTITGGDLTDIQWKQYFPTEDYSSTCEPFQTIPEETTL
                     IPSTGGGDTPEFLAACTDSQTPSCSEPTYEITDLFCIDRGSYGLYTLSPGTTFEVLTP
                     GFNCINEDMNSDGEPRISCTGPADTNFLVSFCNSACTNSLETSYQCQAGFGLNAAEGC
                     CAPVTSASNGCITQTLALPECE"
     gene            complement(10496..11668)
                     /locus_tag="PL000_15255"
     CDS             complement(10496..11668)
                     /locus_tag="PL000_15255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNC90296.1"
                     /translation="MKDARFNQYFQKSFLLQIITAWTVLVLAVYFIHENWLQNNLPAF
                     ALLWAVGILGLQTSIRWRWNNLNDEEKDFEARKISNLIYHAVMNTSTDSIAVVDPFGN
                     YIFANKQTAVLHGYSSSEEFIGVSAFKFFSLDEVARAIKYMEMTRADGVIRNIEFNLL
                     RKDGSTFPAELSAALVKNELGLPVAFIAVTRDITERKRITDQLRDVNEQLRKQLGEIE
                     KLQSILREQAIQDPLTGLYNRRYMEEVLKQEFARASREGKPFSIVMLDLDNLKTLNDT
                     HGHVVGDEALKLLARQIKRLTRIEDTACRYGGDEFLVILHNADEMTAQNRIEELTKRI
                     QEARITSVGEEMPISFSAGSATYPRNGDTVEQVIQFADTALYNVKASRKKPRVTSQ"
     gene            11719..12360
                     /locus_tag="PL000_15260"
     CDS             11719..12360
                     /locus_tag="PL000_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNC90297.1"
                     /translation="MTRNYLVPLTEIRREQEVLKSRFIATLAPAFSIDEARAFMARMK
                     KEFSDASHNVPVYVIGGGNTVTEYFSDDGEPSGTSGKPALAVLRGSGLGDVVLVITRY
                     FGGTLLGTGGLVKAYTEAAQSVVNAVERGRRVPVHVVMMAIPYNLLERVRLLVSKDRG
                     KILGEDYAGDITLTMQFPVENFDTFQADMRELSAGKIQAEVIESKEEIVSASA"
     gene            complement(12309..13532)
                     /locus_tag="PL000_15265"
     CDS             complement(12309..13532)
                     /locus_tag="PL000_15265"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNC90298.1"
                     /translation="MDYVQRAFEHIRHLSETIGGRGSCGPNHRRAAEYSAEALRSAEV
                     SQIEFEPFRGMASTYLPFALAFASALTGTCLALFFGNRLTFGIAALMNFLGAWAMFAE
                     SEFASNWTRWLIPTAETINVTGAVSPQTKPKRQIILCSHLDTHRTPIFYSSTAWQKAF
                     GVSVTGAFLSMVLSALTFGLGTLLDLNEFRWVGLLFGLVQMYALILVISAEFTPFSPG
                     ANDNASGTGIILALAERLRAEPLTNTKVNFIFTDCEETGAHGILAYLKKHAEAFGKDA
                     VYIILDEVGAGQLKTVTEDGLIRKSKTHPRALELARNANFGLTQSLIEAKGEAYTDAL
                     PATKQGLIALPISSAFPNPEQAVSHWHQMSDRIEFIDRQSLQDTFTFTWNILQAVDHQ
                     ADAETISSFDSITSA"
     gene            complement(13549..16350)
                     /locus_tag="PL000_15270"
     CDS             complement(13549..16350)
                     /locus_tag="PL000_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HNC90299.1"
                     /translation="MNLTTILPASRIRDRLIDRIAYANDASYFRLVPQAVVQPNSIGE
                     IQSLFKFSQQNKIPMTFRAAGTSLSGQAVTDGILVDISKHWGEFSVEADVNRIRFQPG
                     IVGGFLNNVLKPHRRRIGPDPASIDACMMGGILANNSSGMCCGTTENSYKTIHSMTFV
                     LPNGFVLDTTDPNANQLFENEQPQIAKGLLELKKRVTSNLKLSTRIAKRYQIKNNNGY
                     LLNAFLDFNSPLDILVHLLIGSEGTLGFIAEGVLHTLPDFTHKYTGQMYFKNIRDAAA
                     AIYPIKQSGARAAEIMDRESLRAVENNAGVPAILKQLPNGAAAILVEYQAADSESMLQ
                     FKKEAQRVIGKLKLIHDVEFTDDPIQQAALWKPRKGIIASVGAMRPRGTTSVNEDVAF
                     PVEHLADAVTDLRHLFDDFGYTEGVIFGHAKDGNLHFLVNQAFDTPEQINHFDNFMQA
                     MVKIVSGKYDGSLKAEHGTGRNMAPFVETEWGSEAYAIMRDLKSLLDPDGMLNPNVLI
                     NDDPKAHVTHLKSLSLVAPEIDKCIECGFCESKCPSRRLTLTPRQRIVVQRELARMRL
                     ADSSSPEFDSVSTDYTYAGIDTCALDGLCGTACPVGIDTGKFIKKLREKRVTSNKSAE
                     WIVENFATVEKAVGIGVSLGHAAEKVIGANGVKSISAAAEKVTGTRLPKWNHSIPHSP
                     KKLSALRGEKEFIYFPSCISRQLGKPTHHSPLSTVFLTIAKRANINLQIPDQTEGHCC
                     GMPFSSKGYKSAYQAALHKTLMQMWEWSEHGKYPIVIDTTSCTHTLKTCGEDLTPEDR
                     EIWRQLTLLDGVEFLHDHVLPKLEIHPVNEEVILHPNCSARKLNLDVKMHAIARQCAR
                     SAVVPFALGCCAFAGDRGLTHPELTASATEKESAEVLAKEYGGHYSSNITCEIGMKEA
                     TGKDYVSIVYLVEKASRKI"
     gene            16439..16699
                     /locus_tag="PL000_15275"
     CDS             16439..16699
                     /locus_tag="PL000_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90300.1"
                     /translation="MTLMEQIEKQIRQLPLESQREVLDFVTFLQLRMRSTLPASMEVE
                     RGKRIKAALLNLAELKVFADIKDPVEWQKQIRADRPLPGRSV"
     gene            16696..17064
                     /locus_tag="PL000_15280"
     CDS             16696..17064
                     /locus_tag="PL000_15280"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC90301.1"
                     /translation="MILADSNLIIYAASGNYPALLDWFAENQPVASAVSLVETLGYYK
                     LTTKEKAALEALFAELTVLYPTVEIFQRAVDLRQQRSVSVGDALIAATALHHNLILAT
                     HNPKDFEWIESLSLVDPLAE"
BASE COUNT         4000 a         4082 c         4687 g         4608 t
ORIGIN      
        1 gttgtaaatg gattcggtct gcacgtgttt ggtggagttt tcccaaacgg ctttcaattc
       61 gtgcaatccg cgtgcttcga cgggggcgtc ctgcgccgag actgcaaccc agtggaatgc
      121 aacttcttcg gttgcatccg ctacacgggc gatgtcacgt acatcttgca ggcaggaggt
      181 gcgtttctct cctgtgtgga tgtcgatgac ttccacgccg cagccatcgg ttccgaggtt
      241 gacatggttt ccgtctagtt gcagatcctg ctgtgggtcg cgtgccgcaa gtttgtaagc
      301 aggcggcgct ttcttgagcg catcttcgat gacctgcggc ttgacccgca cgatcttctt
      361 ttcgtgatcc acttccgcgc cgacagattc ccatatctcc agcgcgcgtt tggatggaaa
      421 ccgtacgcca accttctcga tgatctgcaa tgtggcgtta tggattttta ggacgtcttc
      481 cttggtcagc acttccagtt tcaacttggg gttgctgatg gttttgatat tcttttgaga
      541 cataagccct ccgaggcaaa atgtctctct tctctctccg agttaaacct tcaacttgta
      601 acctgtaact tgcaactctt tcccttacgc gtctgccttg ccaaccagtt cttttgcaat
      661 cttcactgcg ccgatggcat cttcagagta gccgtctgcc ttgatcttgg tgacccagtc
      721 acgggtgatg ggagcgccgc caaccatcac tttgatgcga ggtcgcaggc cttctttatc
      781 caactcttcg atcacttcgc gctggcggac catggtggtg gtgagcaacg cgctcatgcc
      841 gatgatctgg gcgtcgatct ccagtgcctt gccgatgatc ttgtcagcgg gttgatccac
      901 accgaggtca accacttcga aaccagaggc gctcagcatg gtgccgacga gagtcttgcc
      961 gatctcatga atatcgcctt caacggtggc aagaacgacc ttgcccatca tttcacgggc
     1021 ggagccgagt ttgagcagtt cgggttccag aacggccacg gctgctttca ttgcttcacc
     1081 agccatcacc agttcaggca ggaacgcttc gccttttccg aattgatcgc caatgtagtt
     1141 aacgcccact acaaagccgt tcgtgatcgc gtcgagcgga tgcatgttca gctcgatcgc
     1201 ctttttggcc agttcggtgg agatatcgga atccccatca atgattgatt gtgccatttc
     1261 tttgtaaagt tcttcagaca tattcaagcc tccttatttt ggttttttca acaaatgagt
     1321 gatttgcttt tctttttctt tcaataactt tgcaggtatg tttaaatgag tttccagcca
     1381 cctgccagat tccatcacat gttccagcga aactttgacc gcaaggtctg cgtcgtgttt
     1441 ttttaacgca tcaaggattg ctgcatgttc atcatgggtc tgtcggacgc tttcatccac
     1501 cagctcgggt ttgcgaaaca gcgcctcgta caatagccag tcaggaaatg cgtttgacac
     1561 aatagtataa acctgaacca tcaatgcgtt tccagaagct tcggcaattg ccgcatggaa
     1621 ttcccgactg agctgccttt cctgaggcat ttcactcaat atgacatgcc tgtccatttc
     1681 ttccatgatc gtttcgattt gtgtaagttg ctgaggcgag atattctccg cagcttcacg
     1741 tgctatcaat gcttcaagca tgaggcgcag cccgtatgcc tccaccactt ctctggcaga
     1801 tatctcccgc accctgacgc cgcggtaggg aacacgctcg gcaattcctt ttgccaccaa
     1861 caagtcgaag gcttcacgga ctggggtcat actgacgccc atttggctgg caatatcttc
     1921 ctgacgcagc cagtcgccag gcgtgtattt gccggcaatg atctgcctgt gaagtgcatc
     1981 gtaaatttcc tctttaagag gtttgtgggt gagttctttt tccatggctt gtcaatatga
     2041 ttatatatta tatataatta gggtgaattt cggaagtaag aattgtcacg aaaattgtca
     2101 catgaaagcc aggatcagtt tgaaaataaa aatcgtgggc cagcaataaa aaattcgttt
     2161 aagttatact cggagtactg gagcacatat gtctgacatt gatctgaatc cgctaaaacc
     2221 tgcaattgaa tcttatattc ccctcggccg ttcaggcttg ttgcctgcgt tgcatgcggc
     2281 gcaaaagatt tatggatggc tgcctgaaga ggttgcgtct gaagttgcaa aatcgctgcg
     2341 tgtgccgctg gcagatgtgc atggcgtgat cgaattttat tcgctctatt ataacgagca
     2401 ggtggggcag aagtttattc gggtttgcac cgacccagcc tgtgcattaa aaggtgcaga
     2461 cggactcctc aagcacctgt gcaaaaacta caaagtggat gcgggtcaaa cgaccgatga
     2521 cctgtctctg accattgagc aaagtccatg ccttgggctt tgcgaccagg cccccgctgc
     2581 acttgtggat gatgacgccg aaacgaatat ttcgcacaat ttccgttcct atgatattgg
     2641 cgtgccgcaa tcgctggtct atggctcgat ccgtgagttg accgcaaact gcggggaagg
     2701 cacaaccacc cttgcgaaat atggtgagta ttccgcctac aagaaagcat taagcgtcaa
     2761 accagaagat gtcattgctg aagtaaaggc cagtggattg gtcggtcgtg gcggcgcggc
     2821 attcccaaca ggtatcaaat gggaaggcgc ggccaaagcc agcgctgatc aaaaatatgt
     2881 catttgcaac gcagacgaat ccgagccagg gactttcaaa gaccgtgttt tactgttgga
     2941 tgatccgcac cgtaccatcg aagggatgtg cattgccgca tatgcgatcg gtgctacaaa
     3001 aggttacatc tacattcgtg gtgagtaccc gtatattgtc cctgttctcg aagaggcgtt
     3061 gaacgaagca agaatggcgg gctatcttgg agaaagattt gatattgaaa tccgcgttgg
     3121 cgctggtgcc tacatttgtg gcgaggagac cgcgctgttt gaatcgatcg aagggaagcg
     3181 cggttttccg cgtgtgaagc cgcccttccc gacgacgcat ggcgtgtttg gcaagccgac
     3241 tgtcattaac aatgtcgaga ccatgtgcaa cgtgccgctc atcattgaaa aaggctctgc
     3301 cgaataccga aagatcggca cggaaaaatc acctggcccg aaattgttct gtgtttctgg
     3361 cgatgtttcc aaaccaggtg tgtacgaagt cccctttggt gtgactttgc gtgaactgct
     3421 tgaaatggca ggcggtgtga caggcaaaaa agcattgcaa tcggtgttgt ttggcggggc
     3481 ggctggcgct tttgccacat ccgaacattt ggacgtgaga atgacctttg aagatctgcg
     3541 cgccgctggc ttgccgcttg gctcgggcgt ggtcatggtt ttcgatgaaa cccgcgacat
     3601 gcgcgatgtt ttacagcgac ttggtcattt ctttgctcat gaatcctgcg gcaagtgcta
     3661 cccctgccag atgggtacac agcgtcaaat ggagattttg aatcgtgtgg cagcggggaa
     3721 aaccctgccc gatgacatga tccgcttgca ggatgtaggc tggacgatga cggaggcaag
     3781 tctgtgtggt ttggggcaga cggctgcaag tgcagtgttg tctgccatga agttatggcc
     3841 tgcacttttt gaaaatgggg gtggaaaaac aaagagtgac aagaagtctg tagccgcaaa
     3901 gccgcgtgca gcctctgcga ggaaatcagt aaaagcaaaa gcggtcaaga aaaccacagt
     3961 aaagaaacct gtcgcgaaga aaacggtgaa gaaggctgtt aaaccttcca agccgaaggc
     4021 gaagaaaact gctacgaaaa aatccaccgc gaataaaacc gcgaagaaac gctaaaggtt
     4081 aagccatgac tgaaaccgta aatctaatta ttgatggaca ggaagtcagc gctgagcaag
     4141 gtagaaccct gcttgaagta gcccgtgcaa tgggaaagaa tatcccaacc atctgttttc
     4201 atgatgcgac gacagccaac gccctttgta gaatctgtgt cgttgaggtg gaggggcaga
     4261 gagtgctcca gccagcatgc atcgtcaaag caggagcagg catgaaggtc cagacgcgaa
     4321 gcgagaaagt catccgcgcg cgaaggacga ttttggaaat gcttgcctct accatggacc
     4381 tttccgaggc ggcggatatc cagcagatga tggttgagta tggtgcttca tcggagcgtt
     4441 ttcccgatgc tgaacggcgt gagtcggagg tcaaggacga taacccgatg tacgtccgcg
     4501 attactcgaa gtgcttgctg tgttggcgct gtgtgcaggt ctgtgcggaa gatgcgcaat
     4561 atacttttgc gatcaatttc gatgggcgtg gatttgaaac ccagatcggc acgttctttg
     4621 acaaagccat tccgcagaca tcctgcgtga tgtgcgggca atgcgtgggt gtgtgcccaa
     4681 caggcgcatt gaaaccgaag cgtgaatttt tattggaaca aggcatgagc ccgagcgaga
     4741 tcagtgtgat gaacacgggc cgcaagcaaa ggaagccgcg atgattaaat ctgaccgagt
     4801 taccacaaca acctgtccct attgcggagt gggatgcact ttgcaattgc acgtcaaaga
     4861 cgattttatt tacaaggtga ccagcccctt tgattcaccg gtcaatcagg ggaacttgtg
     4921 cgtgaaaggc cgctttggct acgattttat ttatcacccc aagcgtacta caacgccttt
     4981 ggtgcgccgt tatttgcttg aaggcaaacc aaaacctgca ggccgtgaac aggtgtttgc
     5041 cgtcagcaca gatggcaagc ccattcctga ttcgaagatc tccaatgatt gggattgggt
     5101 cgaaacagat tgggatactg cgctgaattt cgtggccgat aaccttgtcc gcatttacca
     5161 gcgtgatggt tcggatgcga tggcggttta ttgctgtgca aaagccacca acgaagataa
     5221 ttatctttta cagaaaatgt accgcgccct attccgcacc aacaatgtgg atcactgcac
     5281 acgcctgtgc catgcagggt ctgtggtggc gcttcagcag gcagttggtt cctctgcgat
     5341 gagcaacacc gcttcacagg tcatcatgaa cgatgtgttc atcgtgacag gttcgaacac
     5401 ttcggagaac catcccatta ttgcattaca gatgaaggaa gccgtgcaaa aacacggagc
     5461 gaagatgatc gtgatcgatc cgcgccgcat cgatttggtg gacatggccg agatgtggct
     5521 tccgctcaag cctggcacaa atgtgcctgt tttctccgcc atggcgcatg tgatcgtgaa
     5581 agagaacctg gtcaatcacg agtttattgc caatcgtacg gaaggctatc aggattttgt
     5641 ggaatcgctt gaaaaattca cccctgaata tgccgaggag atttcaggtg tgcctgccga
     5701 agaaattcgc aaggcggcgc gcatgtatgc aggtgcgaag aatggcgcca tttattgggg
     5761 catgggtatt tcccagcttt cccacggtac agcctctgca atggccttga tccacctggc
     5821 atttctcacg gggcatatcg ggcgtgacgg tacggggctc aatcccttgc gcggacaaaa
     5881 taatgtacag ggcgccagcg atatgggatc catgcctttc cattaccctg gttacatgcg
     5941 tgtggataac cccgagaatc gtgcaaaatg ggaaaaagcc tggaacattg aagaaggtgg
     6001 tttgagcctg aaacttggcc tgaccacaac tgaaattctc agccatgctc acgaaggcgg
     6061 ggttcgggct ttgtacatca tgggtgaaaa cccgatgatg tcggagccga atctcaacga
     6121 tacatgcagg catatgatgg aattggaatt cctcgttgcg caggatatct ttatcaacga
     6181 gagtggttcc tttgcagatg tcttcctgcc tgcaactcct tttgctgaaa aagatgggac
     6241 tttctcgaac actgaccgtc gtgtacagcg tgttcgcgcg gctcaggccc cccgtggaat
     6301 ggcccgcccc gactggcaga tcatctgcga tgtggcttta cgcatcgaat ctcgccttgg
     6361 caggcccact gcgaaatggg attacaccaa cccggaacag attctgcgcg aaatgggcga
     6421 tgtcaattcc gattatgcag gcatcaccta cgaccgcatt gataagatcg gtttgatcta
     6481 ccctgtgcct tcgatggatc acccaggtac accgacactc ttcaaagaat ccttcccgcg
     6541 tgggcgtgga aaattccatc agttggatta tgtccctgtg cgtgaagatg tggatgatga
     6601 atatccgttt atcctcacca cggggcgttt gcttgaacat tggcacggtg gaaccatgac
     6661 gcgaaattca tcgctgaatg aaatttaccc tgaagcccgt gtggaaattc atcctgccga
     6721 tgcggagatc catggcattc gaaacgacga ccctgtcaag gtccagagta gacgcggcga
     6781 ggttgttgtc cgcgcgacag tgaccgagaa aaccaccgtc ggtgttgtct tccttccctg
     6841 gcatttccac gaagcggcag caaacctatt gacgaacgat gcgcttgatc cgcaggcaaa
     6901 aattcccgag ttcaaggctt gcgcggtgca agtctttcca gcacgggaga gtgacctgcc
     6961 gaatcctgag attgttttga atagaggcag atattagaaa aagcagccct gagattttct
     7021 cagggctgct ttttgatttg gatgcttgag cgtaaaaagg tttgtttatt cgcactcagg
     7081 taatgcaagg gtttgcgtga tgcacccatt gctggcggaa gtgacagggg cacagcaacc
     7141 ttcggccgca ttcaagccga accctgcctg acattgatag gatgtttcca gtgaattggt
     7201 gcaggctgag ttgcagaaag agacgaggaa atttgtatct gcagggcctg tgcaggaaat
     7261 tcgcggctca ccatcgctgt tcatatcttc gttgatgcag ttgaacccgg gtgtaagcac
     7321 ttcaaaggtg gtgccaggtg acaaggtgta caggccatag gatccgcggt caatgcagaa
     7381 cagatcggtg atttcgtagg tgggttcgct gcaacttggg gtttggctgt ctgtgcaggc
     7441 cgccagaaac tcaggggtat caccaccgcc tgttgaggga atcagggttg tttcttcagg
     7501 gatggtttgg aatggctcgc aggttgagct gtaatcttcg gttggaaaat actgcttcca
     7561 ttgaatatcg gtcaggtcgc cgccagtgat ggtgcaggct ttttgcaccc atgatgctgg
     7621 agtgatatcc caaaaaatga tgttgtcgtt gtttcccaaa gatgccagaa aaacacctga
     7681 cggatcaaaa tcaatcgtgc cgatgttgcc gccatgtccg accattttgc tgatcagaac
     7741 ggatacttca gggtttgaga tattccaaag gtttacatac ttgtcgtaac tgccagaggc
     7801 caattgagtt ccatcagggc tgaatgccac agatgagatc cagtcagagt gattggccag
     7861 agtatccatt ttttgcgggt tgatgggatc atccacattc cacaagataa tggttttatc
     7921 cttgccagca gtgacgagca ggctgtcatt aattgggcta aatgcggctg cgagaacctg
     7981 attggagtgg ccttccaaca ctcccatgcg tttggtgttg gtgacgtcag aaatatccca
     8041 aagaatgaca ttgtttgctg ctatgcctgc cagaaggttg ccatctgcat tgatggtcat
     8101 gtcactgatc tcattggatt cctgtgaaag tgtggagatc ttaacaggct ttttggggtc
     8161 gcttacatcc caaaatgagg ctttttcagt gttgatcgcg agagttttcc cgtcgggagc
     8221 gaattcgatc gcgttgatcg tttctccgct caatttgatc gtgttcagtt tggaaaagtc
     8281 tttcatatcc caaagggtta cagtttcatc gaaattcatg gaagccatca aactgccgtc
     8341 tttgttaaaa agtatgatgc tggcagggcc atcctgagtt ttgattttta ccgggcttga
     8401 aagtttggaa atatcccagc ttgtaagttt gtggtcgctg tcaaccgaaa caaggatgtt
     8461 gctgttgggt tgaatggcca tgtcgatcat tgttccgtca atggcgccga tttccatttc
     8521 ggtttttggt tgagccgaat cccagaccat gatggagcca tcgcggctcc ccgagaagag
     8581 cagattgttg tttgcgttaa acagtacggt gttgacgccc agtgagtgcc cgttgatggt
     8641 gcggattttg tagggtacat acctgtctgt aacatcccac agcacgatct gggagttggt
     8701 ttcgttgctg ctggaggcca gtgtgtttcc atctgcggtg aaggccagac tgttgattga
     8761 aagagagtgt ccgctccaat tgctgtagat cgtgggggct gcagggtcgg tgacgtccca
     8821 caatacaatc agggtgtttg tatctcccaa tgccagtgtg ttttcatcgg ggctcaaagc
     8881 gatggcggtg acctttgatc caaacccatc tgttgtagag aggactgtgg gggcgttggg
     8941 gtcctgcaga tcccaggcga caatgcttcc gtcactgcgt gtgaagaata ggatgttctt
     9001 gttgcggttg aaaatggcac tgttgatatc caggctttcg tccccgcttt ccagttgtat
     9061 cagctcaaca ggcgatttcg gattggacac atcaaataag gcaatcgtgt tgttgacatc
     9121 agcgtgaatg tttaatgatg taaggatttt tccgtccaca gtgaatccga ggttggtgaa
     9181 atatccgtcg gttttgctga gcagtgtcgg ggaggatgga tcggagatgt cccacaggat
     9241 caattcgtag tcggaggaag tcgcaaggat ttttccatca ggagtgaagg ttacattttc
     9301 cacggggcca gtgaattctt tgagcgtgga cagtttcaca ggaaaatcag gatttgacat
     9361 attccagatg ttgacggtgt aatcccagct gcccgaaaca aggatggttt ggtcggggtt
     9421 gagcaccatg gtgttgaccc agtcttcatg gccgagcaaa atttgatgca aaccagggtg
     9481 gctttgcaag atcttggtga tggcactttg ggtgttcacg tcattgattt tattgtcctt
     9541 ggcgtgaagg taggcttcca cactcaggag cagggcctca ttgtagcttt gatcgatctt
     9601 ggacatggcc agcgctgaca attggttgat ctttgcttcc gtttccttga gcagtgcaag
     9661 gttttttgcc gtggcttccc gctgttcact ttcatttgcc ctggccgctt cattgacggc
     9721 caatctggca gagttcaagc catacactgc cagtgcaaac atgatcaccg ctgcaatggc
     9781 aatgaagacg gttgtccacc ttctttgctg gtcggtggct tggcggcttt ttaagatgta
     9841 ttcacggtgc aggtcggttg gataggggtc ttttgaagaa ttgagcgcca actggctttc
     9901 tgcctcatgc agatcctgtc cgcgcaggag aaagctcttg tctttgtttt tattcttcca
     9961 ctccaacgcg cggataagca ggcgtttatg ggcctcaacc cattcaaaat ctgtgttgat
    10021 gccttgcagt agttttttga aggatgtatc gaagtcatcc tgctcgcgga agaatatcca
    10081 attgaggtgc gagaacgctg cggggacttc atcctcttta acgtctcgga tcatcaacgg
    10141 aatcaggcgt ttgttgttct tgaccgcatg agcaatttca tctccgcaga cctttgactt
    10201 ggccgaatca ggactgagaa ggaatagaaa cgcatctgat tcctcaattc cgttttcgat
    10261 cttcttcatg aagtctactg ttggggggat gccttcccaa tccacccaga actcgatctc
    10321 acttttttgc agttcctggg tcaggtgttt tgtaaattct gtgtctttgc gggaatatga
    10381 aacaaatact ttggccatgt tctgctccat tcgggccgtt aattgacaat tcctataata
    10441 acggcttgat tttacattga tttatgtaaa cacgggcaga ccttgcattt tcagttcatt
    10501 gcgatgtgac ccggggcttt tttcgagatg ccttgacgtt gtacagcgcc gtgtctgcaa
    10561 actggatgac ttgttcaacc gtatcgccat ttctgggata ggtggctgat ccagcggaga
    10621 agctgatcgg catctcttca cctacactgg ttattctggc ttcttgaatg cgcttcgtca
    10681 attcttcgat acggttttgg gcggtcattt catcggcatt atgcagaatg accagaaact
    10741 cgtcgccgcc atatcggcag gcagtgtctt cgatccgagt caacctcttg atctgtcttg
    10801 ccagaagctt tagggcttca tctccaacaa catgaccatg ggtatcgttg agggttttca
    10861 agttgtccaa atcgagcatc acaatgctga atggcttacc ctcgcggctg gctcgagcaa
    10921 attcctgttt gagcacttct tccatgtagc ggcgattgta cagccccgta agcggatctt
    10981 ggatcgcctg ctcgcgcaga atggattgca gtttttcgat ctccccaagc tgcttgcgca
    11041 gctgttcgtt cacatcgcgg agttgatctg taatgcgttt acgttcagta atgtcgcggg
    11101 tcactgcaat aaatgccaca ggcaggccga gttcgttttt taccagtgct gcgctcaatt
    11161 cggcaggaaa agtcgacccg tcttttcgca ggaggttgaa ttcgatgttc ctgatcactc
    11221 catccgcgcg ggtcatttcc atgtacttga ttgctcttgc aacttcatcc aatgaaaaaa
    11281 atttaaaagc actgaccccg ataaattctt cagaagaaga atacccatga agtaccgcag
    11341 tttgcttgtt tgcgaatatg tagtttccaa acgggtctac aacagcaatg gaatctgttg
    11401 aggtattcat gaccgcatgg taaatcaaat tgctgatctt tctggcttcg aaatcctttt
    11461 cttcatcgtt taggttattc caccgccagc gtatcgaggt ctgtaagccg agaattccca
    11521 cggcccacag cagggcaaag gcgggcaggt tgttctgaag ccagttttcg tgaataaaat
    11581 aaacggcaag aaccagaaca gtccacgcag ttataatttg aagcaggaat gatttttgga
    11641 aatattggtt gaatctggca tctttcatct tgccctcgcc tttaagtttt gtgaattata
    11701 cgccttttgg aaacgcccat gactagaaat tatctcgtcc ctctcaccga aattcgccgt
    11761 gagcaggaag tgctgaaatc gcgcttcatc gccacgcttg cgcctgcttt ttccattgac
    11821 gaagcacggg ctttcatggc gcgtatgaaa aaagaatttt ccgatgccag ccataatgtg
    11881 cctgtgtacg tcattggcgg agggaatacg gtgacggaat atttttcgga tgatggggaa
    11941 ccgagcggga cttcggggaa gcctgctctg gcggtcctgc ggggcagcgg acttggcgat
    12001 gtggttttgg tcatcacccg ttattttggc ggcaccctgc tcggtacggg cggactggtc
    12061 aaagcataca cagaggctgc gcaatctgtc gtcaacgccg tggagcgcgg acgtagagtc
    12121 cctgtccatg tggtgatgat ggcgattccc tataatctgc ttgagcgggt gcgtctgctg
    12181 gtctcgaaag accgcggaaa gattctgggt gaagattatg cgggggatat cacacttacc
    12241 atgcaattcc cagttgagaa tttcgacacc tttcaagcgg acatgcgcga actctcagca
    12301 gggaagattc aagcggaagt gatcgaatca aaggaagaga tcgtttcagc gtcagcttga
    12361 tgatcgactg cctgcaaaat attccacgta aaagtgaacg tgtcttgcag tgactgtctg
    12421 tcaatgaact cgatgcggtc ggacatttgg tgccagtgag aaactgcctg ttcagggttg
    12481 gggaaggctg aagagatggg cagggcgata agcccctgtt tggtggcggg cagggcatcg
    12541 gtgtaggctt cacctttggc ttcgatcagg ctttgtgtca acccgaaatt tgcatttcgt
    12601 gccaattcga gcgcccgtgg atgcgttttg gattttcgga tcaacccatc ttctgtaacc
    12661 gtttttagct gccccgcgcc gacttcgtcg agaataatat aaacggcatc ttttccaaat
    12721 gcttctgcat gtttcttcaa gtaggcgagg atgccatgtg cccctgtttc ttcgcagtcg
    12781 gtaaaaatga agttgacctt tgtgttcgtc agcggttctg cacgaaggcg ttctgcaagg
    12841 gcgaggatga tgccagtgcc tgaggcgttg tcgttggctc ctggtgagaa gggggtgaat
    12901 tcagccgaga tgaccaggat caaggcatac atttgcacca acccaaaaag caggccaacc
    12961 catcgaaatt cattgagatc cagcagggtt cccaatccaa atgtcagcgc gcttaatacc
    13021 atgctcagaa acgctccagt gacggaaacc ccaaaggctt tttgccaggc tgtggatgaa
    13081 tagaatatcg gggtgcggtg cgtgtcgaga tgagagcaca ggataatttg tcgtttgggt
    13141 ttggtttgcg gagaaactgc cccagtgacg ttaattgttt cggcggtcgg aatcagccag
    13201 cgggtccagt tggaggcgaa ttcactttct gcgaacatgg cccatgcgcc gagaaaattc
    13261 atcagggcgg cgattccgaa tgtgaggcga tttccaaaaa ataaagcgag gcaggttcca
    13321 gtgagtgcgg aggcaaaggc cagcgcaaaa ggcaggtagg tggatgccat gccgcggaac
    13381 ggctcaaact caatttggct tacctctgct gatctcaagg cttcggcaga atattctgcc
    13441 gcccgccgat ggtttgggcc gcaactgccg cgcccgccga tggtttctga aagatgacga
    13501 atgtgctcga acgcgcgctg gacgtaatcc attttgggtg ccttcgtgtt agatcttcct
    13561 gcttgctttt tccaccagat atacgatgga aacataatct ttgcctgtgg cttctttcat
    13621 gccgatctca caagtgatgt tggaagagta gtggccgcca tattcttttg caaggacttc
    13681 ggcgctctct ttttcggtgg cagatgcggt gagttcgggg tgggtcagtc cgcggtcacc
    13741 tgcaaaggca cagcagccaa gcgcaaatgg gaccactgcc gatctggcac attgcctggc
    13801 aatggcgtgc atttttacat cgaggttgag tttgcgggcg gagcaattgg gatgcaggat
    13861 cacttcttcg ttcacagggt ggatttcaag cttgggcagg acatggtcgt gtaaaaattc
    13921 cacgccgtcg aggagggtga gttgacgcca gatctccctg tcttcggggg tgaggtcttc
    13981 gccgcaggtt ttgagcgtgt gtgtgcagga ggtggtgtct atgacgatgg gatattttcc
    14041 atgctcagac cattcccaca tttgcatgag ggttttgtga agagctgcct gatatgcaga
    14101 tttgtatccc ttggaagaga agggcatgcc gcagcagtga ccctctgttt gatcggggat
    14161 ttgcagattg atgttggctc gcttggcgat ggtgaggaaa actgtggaaa gtggtgagtg
    14221 gtgagtaggt ttgccgagtt ggcgcgaaat acaggagggg aagtagataa attctttttc
    14281 accacggaga gcactgagtt tttttggaga gtgcgggatg gagtgattcc acttggggag
    14341 tcttgtgcca gttactttct ccgcggccgc tgaaatagat ttgactccat tcgcgccaat
    14401 tactttctct gcggcatgtc cgagtgaaac gccaattcca accgcttttt caaccgttgc
    14461 gaaattttct acaatccact ctgcagactt gttacttgtt actcgtttct cacgcagctt
    14521 ctttataaac ttccccgtgt caattcccac tgggcaggca gttccacaga gaccgtcgag
    14581 ggcgcaggtg tcgattccag cgtaggtgta atctgttgaa acagaatcaa attcgggtga
    14641 ggatgaatcg gcgaggcgca ttcgggcaag ttctctttgg acgacgatcc tttgtctggg
    14701 agtcagagtc aacctgcggc tgggacactt cgactcgcag aatccacatt cgatgcattt
    14761 gtcaatttca ggcgcaacga gtgagagcga cttcaagtgg gtgacgtgtg ctttcgggtc
    14821 gtcgttgatg agcacgtttg ggttcaacat tccatcaggg tcgagcaatg atttgaggtc
    14881 acgcatgatg gcgtaggctt cactgcccca ttcggtttcg acgaaaggcg ccatgttgcg
    14941 ccctgtgccg tgttcggctt tgagagagcc atcgtatttg ccgctgacga tcttcaccat
    15001 cgcttgcatg aaattgtcga agtggttgat ctgttcaggg gtatcgaaag cctggttgac
    15061 gaggaaatgc aagttgccat ctttcgcgtg gccgaaaatg acaccctcgg tatatccgaa
    15121 atcatcgaag agatgacgca ggtcggtaac ggcgtcggcg agatgttcaa cgggaaaagc
    15181 gacatcttcg ttgaccgagg ttgtgccgcg cggacgcatc gcgccgaccg atgcaatgat
    15241 gcctttgcgc ggtttccaca atgcggcctg ctggatggga tcgtcggtga attcgacatc
    15301 atgaatcagt ttaagcttgc cgatgacgcg ctgcgcttct ttcttaaact gaagcatgga
    15361 ttctgaatct gcggcttgat actccaccag aatcgccgcc gcgccattag gcagttgctt
    15421 gagaatggca ggcacgcccg cgttgttttc caccgcgcgc agggactccc tatccatgat
    15481 ctctgccgcg cgcgcacccg actgtttgat ggggtagatc gccgccgccg catcgcggat
    15541 gtttttgaaa tacatctgtc ctgtgtattt gtgggtgaaa tcgggcaagg tgtggagcac
    15601 gccttcggcg atgaatccaa gcgtgccttc agagccgatc aacaaatgga cgagaatatc
    15661 gagcggagaa ttgaaatcga gaaaggcatt gagcaggtag ccgttgttgt tcttgatttg
    15721 atagcgtttg gcgatgcggg ttgaaagttt caggttggag gttacacgtt ttttgagttc
    15781 gagcagacct ttggcgatct gcggttgttc gttctcgaaa agctgatttg cattcgggtc
    15841 ggttgtatcg agcacaaatc catttggcag aacaaaggtc atcgagtgga tggttttgta
    15901 cgagttctcg gtggtgccac agcacatgcc gctggagttg ttcgcgagga tcccgcccat
    15961 catgcacgca tcaatggaag cagggtcggg gccgatgcgc cgtctgtgcg gtttgagcac
    16021 gttgttgaga aatccgccca cgatcccagg ctgaaagcgg attcggttta catcggcttc
    16081 cacgctgaat tccccccaat gtttggaaat atccaccaga atcccatctg tcactgcctg
    16141 ccctgaaaga ctcgtccctg ccgcgcggaa ggtcatcggg attttattct gctggctgaa
    16201 tttgaaaagc gattgaattt ccccgatcga attcggctgc accaccgcct gcggcaccag
    16261 ccgaaagtac gaagcgtcat tggcataagc gatgcggtcg atcaaacggt cacggatgcg
    16321 cgaggcgggg aggatggtgg tgaggttcat gggcatatta taagactgtg gggattttgc
    16381 gaaaacattt tgacagcaaa agttgcggtt ataattaatt catataggag tacactctat
    16441 gactctcatg gaacagattg aaaaacagat ccgccaactt ccgcttgaaa gccagcgtga
    16501 ggttttggat tttgtcacct tcctgcagtt gcggatgcgt tctacgctgc ctgcttccat
    16561 ggaagtggaa cgtggaaaac gtatcaaagc cgctttgcta aacctggctg agttgaaagt
    16621 ttttgctgat atcaaagacc ctgttgaatg gcagaagcag attcgcgctg acagaccgct
    16681 tccggggcgt agtgtatgat ccttgctgat agtaatctta ttatttacgc agcatctgga
    16741 aattaccccg ctcttctgga ttggtttgca gaaaaccaac ctgttgcttc ggctgtgagt
    16801 ttggtggaaa cattgggata ttacaaactt accaccaaag aaaaggccgc gcttgaagcc
    16861 ttgtttgctg aattgaccgt cctttatccc actgttgaaa tatttcagcg tgcagttgac
    16921 ctgagacagc agcgttccgt ttctgtaggc gatgctctaa tcgctgctac tgcacttcac
    16981 cacaacttga ttcttgcgac gcataaccca aaagatttcg aatggatcga atcgctgtct
    17041 ttggtcgatc cccttgctga ataattaaat ccccaattat tacttaggca gagctgagag
    17101 agcaagaaaa tagatgactt gccattcatg aatttacaaa tggatggcaa gtcattcgtt
    17161 tatttgtaga tttcattcgt ggaaggtggt ctcactccct cgatttggac aaaacgtggg
    17221 tcgtttgaat gtcgaggtct tccaatattg gagtaggcgg tgtcgcttgg ctcgatacag
    17281 tggcacaaaa aatatcagca tcttcttgac ctccaaatac ggcttgtaac tttacgccca
    17341 gtattgacta gacaactttc ctaaagtaca atcgcgc
//
LOCUS       DAOQLC010000095        17062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1200_length_17062_cov_26.172047, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000095 DAOQLC010000000
VERSION     DAOQLC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1200_length_17062_cov_26.172047"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PL000_15285"
     CDS             <1..773
                     /locus_tag="PL000_15285"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNC90302.1"
                     /translation="ALHLTRCTSMAVNEERTADGHVLAAHNEDWVPEDEDGVLVISAK
                     PDKEPPFLAMTYGGLLPNVGFNAFGIAQLIDSVYPSDSRIGIPRLVVARAVLSSHRIS
                     GAIGRTLVQHRAAGYNHLLIHESGEMYSIEVSARKFEILYAHDGYMVHTNHYLDPQMK
                     QIEKDPEELLSSRVRYFRASRLLRQNGQHTIKSLQAIQKDHTNIPNSICNHNIEGLDP
                     LDREKTISALVIDLTSREMHITWGNPCQNAYHTYHLNA"
     gene            886..1758
                     /locus_tag="PL000_15290"
     CDS             886..1758
                     /locus_tag="PL000_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNC90303.1"
                     /translation="MRDLTNEILELIRRTSSSLPPDVEKRLRESIEKEEPGSAARGAL
                     ETILKNIDLSRANSTPICQDTGTPIFYVHYPVGWSTIKLKEQIRSAMAEATKRSYMRP
                     NAVDAIYDKNTGNNLGGDDFPYVHFEETEGDDLTIELMLKGGGCENVGRQYSLPDNSV
                     GAGRDLAGVRKVVLDAVQKAQGQGCAPGILGVAIGGDRGSSYIKSKEVLFSEMGTRNK
                     DPKLAELEERLTSEANQMGIGPMGFGGKTTVLDTKITGLSRLPASYFVSVSYMCWAYR
                     RRKMTVKGEEITYD"
     gene            1804..2430
                     /locus_tag="PL000_15295"
     CDS             1804..2430
                     /locus_tag="PL000_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNC90304.1"
                     /translation="MKQIKTPITDEVIRELKVGDAVSLSGMMVTGRDAAHKWMIETFI
                     KKTRAPQGDDLQVYEELKKLLAGSVIYHCGPVVTGLDTKEYKIIAAGPTTSIREEPYQ
                     ADVMKHFNVKGVIGKGGMGAKTLKGCQDTPGVYFHAIGGAASFLAQTVKKVQGVFKLD
                     FGVPEAMWVIEVQDFPVVVTMDSHGGSQHSVIDDSSKKVLDDLLAQPY"
     gene            complement(2501..2863)
                     /locus_tag="PL000_15300"
     CDS             complement(2501..2863)
                     /locus_tag="PL000_15300"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HNC90305.1"
                     /translation="MDSNEIQPSSPLNTKSILSLVFAVLTLLTFCTGWLPVPFTGILC
                     FPASFLLGILALTYGTISLRQIRQRNETGHPMAWTGVIIGGFVFVCVLCMLIAIISIF
                     LYSPDLLPTPPFLQNPQI"
     gene            complement(2907..2981)
                     /locus_tag="PL000_15305"
     tRNA            complement(2907..2981)
                     /locus_tag="PL000_15305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2944..2946),aa:Pro,seq:ggg)
     gene            3110..3688
                     /locus_tag="PL000_15310"
     CDS             3110..3688
                     /locus_tag="PL000_15310"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNC90306.1"
                     /translation="MSDMIEVVIDSIRVHLMTPQRVVVLKQTNAERYLTIWVGPYEAE
                     AITVALQEVEMSRPLTHDLLKNIFTTFNARILRIEITELQGEVFFGNIIAEVDGRELR
                     IDSRPSDAIAIAVRAHVPILVHAHVMESAGMAPDQDMPDTSPTTPLARREAPAPLSDD
                     ANKRLSVFKDFFDKLDIDNPDRDKPDSTPPLD"
     gene            3698..5104
                     /gene="dnaB"
                     /locus_tag="PL000_15315"
     CDS             3698..5104
                     /gene="dnaB"
                     /locus_tag="PL000_15315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC90307.1"
                     /translation="MTDSLPETFPPYGDDSSSAAPASSTSSSIPHSREAEEAVVGAVL
                     INPEIYYDVAQFLSPDDFYIHRNKFVWEAFTRLHEQRIPVDLLTLSEELDRVGQLAEI
                     GGSAYLTSLINQVPSSLNAESYGRIVEGHSIRRKMINAANKIASVAYNEASTVEDVMN
                     EAEKAVFNVSERRLKHDLQPISTVLSDYYDRIDDLAKRPDEIHGVPTGFIDLDRLLTG
                     LQPSDLLIIAGRPGQGKTGFLLSIAKNAALTHKKHVAIFSLEMSNEQVVQRLIAQETG
                     IDSQRLRNGKLHEHEWPLFTHAIEVFSDTHIYLDDTPAITPLQLRTKCRRLHMEYGID
                     LVIIDYLQLMGGDSRNDNRVQEVSHISRSLKVLARELNVPVLTAAQLSRAVEQRTDKK
                     PVLSDLRESGSLEQDADIVMFIYRPDQYEKDSEKQNVAEIIVAKHRNGPVGSVEMIFR
                     GAQAKFENAATRVFRPNE"
     gene            5177..5833
                     /locus_tag="PL000_15320"
     CDS             5177..5833
                     /locus_tag="PL000_15320"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HNC90308.1"
                     /translation="MAAFSGFTSSETFTQIPDSFIRLLGEIEDVAELKVALHAIWRIE
                     HIEGNFRALRESDFTAEMLGLEIKEIKRGLKKCLERGTLLKAENESEVLYFLNSPRGR
                     LAVDSFTKGKLAGIASATRERSNLFKLYEENIGPLTPLVADVLKDAEEIYQAEWIAEA
                     IGLAVENNKRNWKYIEAILKRWKEEGRAEKQDRRNDKKDGQQSLSRKIEQLRKRADKK
                     "
     gene            5772..7145
                     /locus_tag="PL000_15325"
     CDS             5772..7145
                     /locus_tag="PL000_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC90309.1"
                     /translation="MASRASAERSSSSASAPIKNDLPGDPNCPHCHGVGYLRSDVPVG
                     HAEFGKLEICTCRRSKVADNIRDRLFAMSHLDELKDLTFESFKPRGRKGLGEMQARSI
                     EMAFNHAQHYAKSLNGWLLLQGGYGSGKTHLAAAVANFVVGMGVPTLFLTVPDLLDAL
                     RFAYGSEDTTFEERFEQIRSAKLLVLDDFGTQNATGWAQEKLFQIINYRYINKLATVV
                     TTNLSLDEIEPRIRSRLSDPELVSPVKMSAPDYRRPMEDTSHPELSSLDLLSSRTFGS
                     FEDRAAEKLTPDEHKSLQKALKIAHSFSEKPKGWLVFEGTYGCGKTHLAAAIANYRAG
                     LGDPPLFVMVPDLLDHLRAAFSPNSGTSYDRRFDEVRTAGLLVLDDLGAQSATPWARE
                     KLHQLLNYRYNAELPTVITVAKENLDQGQIDERIITRMLDERLCEYIEIKAPAYQGKG
                     KRKIGRK"
     gene            7374..8225
                     /locus_tag="PL000_15330"
     CDS             7374..8225
                     /locus_tag="PL000_15330"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HNC90310.1"
                     /translation="MLPSYLLSLREGLEAALIIGIVLGALQKIRRTDLAPMLWYGTFA
                     AIGVSILTAILLTIFGLSLEGSAEQIYEGITMFLAAGLLTWMIFWMSQQARTLKAELE
                     ADVNKAAASRNGRALFWMAFVAVVREGVELAFFITAAFFAGNTENVGTNTIQTLAGVV
                     LGLSTSILLGWSLFATAVRLDLRRFFQVTGFILILFAGGLVAHGIHEFNEVGWIPNII
                     EHVWDVNAIVDEKSVLGELLKTLFGYNGNPSLTEMIGYFVYLAVVAVFFQRSNTKKDV
                     MVAQPSA"
     gene            8341..9009
                     /locus_tag="PL000_15335"
     CDS             8341..9009
                     /locus_tag="PL000_15335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC90311.1"
                     /translation="MNTKYEAYEQPGITVDLAIFTVNNNKLKVMLVRRAEDPYKDFWS
                     LPGGFIKQGESLEEAANRVLNEKTGVKNVYMEQLYTFGDPARDPRSRVITVTYFALIP
                     WAQLAQPASEKVADLAWSHIDRVPKLAFDHKMILDYAVKRLRAKASYSNIVYGLMPVQ
                     FRLSELQTMYEIIINDKLDKRNFRKRMLSTGLLQETGEKDLTGAHRPAMLYQFKKMEI
                     AFLD"
     gene            9017..10036
                     /locus_tag="PL000_15340"
     CDS             9017..10036
                     /locus_tag="PL000_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90312.1"
                     /translation="MKKQTLPLPEFFESARVGEIWKVDYASRAEQARQYARQHDLKPA
                     SAASEKISLLVIDAQNTFCMPNFELFVGGRSGHGAVDDNVRLCEFIYRNLGRLTHIIA
                     TMDTHMSQQIFHPIFFVDPEGGHPTPYTDIHLADLISGRWKFNAALAPRLNIAPEYGQ
                     QMMIHYAESLEKKGKYALTIWPYHAMLGGIGHALVPALEEAIFFHSIARFAQPDFEIK
                     GNKPFTEHYSVIGPEVLTGPMGETLGSHDTTFIQHLQEVDRLYIAGQAKSHCVAWTVS
                     DLLADIQDADPSLAEKVYLLEDCSSPVVVPGVVDHTEAANEAYIRFAQAGMKVVKSTD
                     EISSM"
     gene            10072..10623
                     /locus_tag="PL000_15345"
     CDS             10072..10623
                     /locus_tag="PL000_15345"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HNC90313.1"
                     /translation="MPNLTRLSKFVSVILRHRANDFDLHLDAQGFTDFAELKRIVIEK
                     STDVYSEEDWQKVLNGELDGKKRFEVKDGRIRAMYGHSKVDPVSYPKVEPPEILYHGT
                     TPQAEKNIRREGLRSMKRQYVHLSSNLERANSVGSRRTEHVTILVVRAREAAVAGVEF
                     YAPEPEHFLARSIPPNFIDFPQN"
     gene            10632..11975
                     /locus_tag="PL000_15350"
     CDS             10632..11975
                     /locus_tag="PL000_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNC90314.1"
                     /translation="MSIFDGKRLTNETFKLDIDRMRRGWYSDKYFENINRMLTALSKE
                     NYNYSGSFPILPNGISPEQIAVGDIEVEMQWFTRRMGKTTVVGVDKSLEMLRHCTGYF
                     DGDTFVDTSDRLEVWAVQDGSIVKFEGDPTKIQPVIKVRGRYRDFALLETPTLGILTR
                     SSRVATNVYETLVASRGKPVLFFPARFDVHEVQAADGYAYNIALLRFNKDHAQELGAF
                     VSTDAQGDWWGGAGGGTVAHAAIASFLGDTAEAMMQFAHILPARIPRIALVDFNNDSV
                     RDSLRVLDAMFAKYRELMDAGNREEAEKYKLYGVRLDTSGSLRDVSVQPLGDPALDLG
                     VNPRLVFNVRQGLDNAWESWTLPTSWKDAAREFCHNVKIVVSGGFNPEKIRKFERLEV
                     PVDIYAVGSYLFNNSNSTSTDYTADVVRVKIHGEWIEMAKMGRKVGENENLERVW"
     gene            complement(12171..13103)
                     /locus_tag="PL000_15355"
     CDS             complement(12171..13103)
                     /locus_tag="PL000_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90315.1"
                     /translation="MPQNKSMPDEHQIEKILSTIQPMPGEGFYSKMKTAPWQVKRTGK
                     TTTMKYKYAFLTLAMLLALTATVVFVPPVRAQVEEWFFVKFQSPNNSNLSFGFVSNEP
                     MKYQLMQPGYLPEIFKDINSASFGDVSELLYKVDDTFLIITQQPAADNDALPDGEAVT
                     INGLPAVLNTNLSGTYQNQPPSSDTNSSGGIYLSSGGDMTPEASLEITSSSQDLQIYT
                     GTLPDGAEVPLEQSQADGDIIIDNLDSESLSLQSQPNVDVTVENLAPIAPESFEYFNA
                     SKLTFIMGGTKIELLTNLPMDELLKIAEQLIPAN"
     gene            complement(13114..13683)
                     /locus_tag="PL000_15360"
     CDS             complement(13114..13683)
                     /locus_tag="PL000_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC90316.1"
                     /translation="MTEQPVLQNIKRWLWQEQEYSITDADAFATLYELHHLTVFRYVY
                     GLSGGPQQDAEDITAETFIRAWTTRHRFTGSKQAALGWILRIARNLIIDSSRRRRVRP
                     EEENIDLESLLDTKSLPEIDIITREQAMVLWKTLSTLPEDLREMLVLRYMLGWQVKQI
                     ALHLDITENNVSVSMRRALNRLQQGWPQS"
     gene            13957..16020
                     /locus_tag="PL000_15365"
     CDS             13957..16020
                     /locus_tag="PL000_15365"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HNC90317.1"
                     /translation="MKRIFISYRRQDSGVFIGRIYDQLKLHFGERNVFRDVYNIPAGS
                     DFREVLNKEVGQCDIFLAVIGRQWVDISSTQGRRRLDDPNDFVRIEVELALSNPNILV
                     IPVLVDNAEMPEVEDLPPSLQPLCYRNAVKVRTDPDFPHDMQMLLQQIKPAKSFISTR
                     VGWLMLALSILLIAGLSFFPMFNGEKDIPSPTAAQTDIPPTPTPLFAPVEPVEPGQVM
                     VLVANMEQVGSTKRDVTRFIVEDLVNHFESDLSSTNIRIREYERFITSNDEALQLAEQ
                     TQAVIVVWGQYDNDNVTVNIQLGSLQSRPAIALDRSLLEQAVNIRVKLTDERQETLAY
                     HILSSLILIHCAENDMVKAMSLIISFDLLDAPGPEPIGNSSAAHVQKAMKAFLSDRKT
                     ALDELNQTVELAPDNPLFYMLRAILYQGMGEFALSNQDLDTALLLAPEDWILPYHLKG
                     GISLLLSDVPNGVEFYTRVIEKTPDSWFPYNQRGYLYFLAGQYDLARSDIEKSIALKP
                     EAEWPYMWGALIALRQGRMEDASFMMSLLLQEQPNPTFINRLMTILYGEATAKLLGES
                     MAAMGHLTLGQYDVAIQDTDGVLAVMPAYTDMYMLRGLSYCNLDNYVDAEAAYTQGLT
                     LDPSFALLHLLRVEVRTKQGNVAGAMEDIDAVQQNNELTANLAPYLAAAQSGEFSCKQ
                     LLRSK"
     gene            16173..16655
                     /locus_tag="PL000_15370"
     CDS             16173..16655
                     /locus_tag="PL000_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90318.1"
                     /translation="MNDQLSKNVIKKIKADYKLEEIPRVAECLGQYGTDKPETERVQL
                     GILNVANGDIPKLQMLVSVALTDYRDLLVQAEYLNDPYNLLQTLLRNLQESNTFTTEE
                     VTETMRATGGTNYRKAFEFLMVMIPQRGKTLNAEQYQLIDKLGRALKLPVKTWKHLAV
                     "
BASE COUNT         4228 a         4126 c         4423 g         4285 t
ORIGIN      
        1 atgcacttca cctcacccgc tgcaccagca tggcagtcaa cgaagagcgc acagccgatg
       61 gacatgtgct ggccgcccac aacgaagatt gggttcccga agatgaagat ggcgtcctgg
      121 tcatttcagc gaagcccgac aaggaacccc cgtttcttgc catgacctat ggcgggctgc
      181 tgcccaatgt gggattcaac gcctttggca ttgcccaact cattgactct gtttatccga
      241 gcgactcgcg cattggcatt ccgcgcctcg tggtggcgcg cgctgtcctt tcttcgcatc
      301 gtatttcggg tgccattggg cgtaccctcg ttcaacatcg cgcggctgga tataaccacc
      361 tgctgattca cgaaagcgga gaaatgtatt ccatcgaggt ctcggcgcgc aaatttgaaa
      421 ttctgtatgc gcacgatggg tacatggtcc ataccaatca ttatctcgac ccgcaaatga
      481 agcagatcga gaaagacccc gaagaattgc tctcttcccg tgtgcgttac ttccgtgcct
      541 cgcgcctgct gcgccagaac ggtcagcaca ccatcaagtc cttgcaggcc attcaaaagg
      601 atcacaccaa catccccaac tcgatctgca accacaacat cgagggactt gatccgctcg
      661 accgcgaaaa gaccatctcc gcccttgtca ttgacctgac ttcacgtgaa atgcacatca
      721 cctggggaaa cccctgccag aatgcgtatc acacttatca tttgaatgcg tagggtgtaa
      781 gaaagtgaaa agaaaaagag tctgtgcgcc cggcacagac tctgaatttt taaatggcaa
      841 attcatgcct tcgggattat cataagcact tctattggag gtcccatgcg agatttaaca
      901 aatgaaatcc ttgaattgat tcgccgcaca tcttcaagcc tgccgccgga cgtggagaaa
      961 cggctgcgcg aatccattga aaaagaggag cccggttccg ctgcccgcgg ggcgttggag
     1021 accatcctca aaaatattga cctttcccgg gcaaattcca cgccgatttg ccaggatacc
     1081 ggcacgccta ttttctatgt gcattacccg gtgggctggt ctacgatcaa gctcaaagag
     1141 cagatccgct ctgcgatggc cgaagccacc aaacggtcct atatgagacc gaatgccgtt
     1201 gatgccatct acgacaaaaa caccggaaac aatctcggcg gcgatgactt cccctatgtc
     1261 catttcgaag agacagaagg cgatgacctc accatcgaat tgatgctcaa aggcggcgga
     1321 tgcgaaaatg tcggccgtca atattccctg cctgataatt ccgtcggcgc ggggcgtgac
     1381 ctggccggtg tgcgcaaagt ggttttggac gcagtgcaaa aagcacaggg acagggatgt
     1441 gcgcccggta ttttaggtgt tgccatcggc ggtgaccgtg ggtcgtcgta tatcaaatcg
     1501 aaggaagtgt tgttcagcga aatgggcaca cgcaacaaag acccgaaact ggctgaattg
     1561 gaagaacgcc tgacgagcga agccaaccaa atgggcattg gcccgatggg gttcggcggc
     1621 aagaccaccg tgttggatac gaaaatcaca gggttgagtc gtttgcctgc ttcgtatttt
     1681 gtatctgttt cgtacatgtg ctgggcctat cgccgccgca agatgacggt gaagggtgaa
     1741 gagatcactt acgattaacg attttcgatt tacgatggtg cggttcgtaa aaaggagaat
     1801 gaaatgaaac aaataaaaac accgatcacc gatgaggtca ttcgtgaatt aaaggttggt
     1861 gatgcggtca gcctgtcggg tatgatggtg accgggcgcg atgccgccca caaatggatg
     1921 atcgagactt tcatcaaaaa gactcgtgct ccgcagggcg atgacctgca agtgtatgaa
     1981 gaattgaaaa aactgctggc tggctcggtc atttaccact gtggccccgt ggtgaccgga
     2041 ttggatacga aggaatacaa gatcatcgcc gcgggcccca ccacatccat ccgcgaagaa
     2101 ccgtatcagg cggatgtgat gaagcatttc aatgtcaaag gggtgatcgg caaaggcggc
     2161 atgggcgcca agaccttgaa aggctgtcag gatacccccg gggtgtattt tcatgccatc
     2221 ggtggtgcag cctcttttct ggcgcagacc gtgaaaaagg tgcaaggtgt tttcaaactg
     2281 gattttggtg tgcccgaagc catgtgggtg atcgaagtac aggatttccc cgttgtggtt
     2341 accatggatt cgcatggcgg cagtcagcat tcggtgattg acgattcttc caaaaaggtg
     2401 cttgacgatc tgctggcaca gccgtactaa ccccacatta gcaaagtgct gacctgaaat
     2461 cgaattacaa actccgcctg tatttcagac gggttttgtt ttatatctgt gggttttgta
     2521 aaaagggtgg tgtggggagt aaatccggcg aatataaaaa gatcgagatg atcgcaatca
     2581 acatacacag cacacaaaca aaaacaaagc cgccgatgat gaccccggtc catgccatgg
     2641 ggtggcctgt ttcgttccgc tgacggatct gccgtagtga aattgtgccg taggtcaggg
     2701 caagaatgcc aagcagaaaa ctggctggga aacaaaggat ccccgtgaat ggaacgggaa
     2761 gccagcccgt gcaaaatgtc aacagggtta acactgcaaa gacaaggctg agaatggatt
     2821 tggtattgag aggggaggag ggctgtattt cgtttgagtc catggaaaaa taaaaccgcc
     2881 ctgtaagggc ggttcctttt gtgctgtcgg ggcgagagga tttgaacctc cgacctcttg
     2941 caccccatgc aagcgcgcta gccgggctgc gccacgcccc gattgagcga agcggattat
     3001 agctgtattt catcattctg gcaaatgcaa tttttcacgg ctccgggtgg caacctgcaa
     3061 ccttccacct tcacccttta tgctaaaata agcgcattgg agttgcctta tgtcagatat
     3121 gatagaagtt gtaattgata gcatacgtgt acacttgatg acaccacagc gtgttgtcgt
     3181 tctcaagcag acgaatgccg aacgctacct taccatttgg gtgggccctt acgaagcaga
     3241 agcgatcacc gtggcattgc aggaagttga aatgtcccgc ccgcttaccc acgacctgct
     3301 taaaaatatt tttaccactt tcaacgcgcg catcctgcga atcgaaatta ccgagcttca
     3361 gggcgaggtc ttctttggaa acatcatcgc cgaagtggat ggacgggaat tgcgcatcga
     3421 ttcgcgcccg tctgatgcga ttgccattgc ggttcgtgcg catgtgccga tccttgtaca
     3481 tgcccatgtc atggaatccg cgggcatggc ccccgatcag gatatgcctg atacatcgcc
     3541 cacaactccg ctggcacgcc gcgaagcccc tgcaccgctc tcagatgatg ccaacaaacg
     3601 tctgtctgtt ttcaaggatt tctttgacaa actcgacatt gacaaccctg accgcgacaa
     3661 acccgattca accccacctc tggattgatc cacatcaatg acagattccc tccccgaaac
     3721 attcccccca tacggggatg attcatcatc cgccgccccc gccagttcga cgtcttcgtc
     3781 catcccgcac agccgcgaag ccgaagaagc cgttgtaggg gcggtcttga ttaaccccga
     3841 aatctattac gacgtagccc agttcctgag cccggatgat ttctacatcc accgcaacaa
     3901 gtttgtgtgg gaagccttca cacgtttgca cgaacaacgc atccctgtgg atttgctcac
     3961 cctctccgaa gaacttgacc gtgtagggca gcttgctgaa attggcggct ccgcctacct
     4021 gacgtcgctc attaatcagg tcccatcttc gctaaacgcg gaatcttatg gccgcatcgt
     4081 tgaaggccac tccatccgcc gcaagatgat caatgccgcc aacaagatcg catccgtggc
     4141 atacaacgaa gcctccaccg tggaagatgt gatgaacgaa gcggagaagg ccgtcttcaa
     4201 cgtcagcgaa cgccgcctca aacacgacct ccaaccgatt tccacggttt tgagcgatta
     4261 ctacgaccga attgacgacc tcgccaaacg cccggatgag atccacgggg tacccaccgg
     4321 gtttattgac ctcgacaggc tgctgacagg tttacagcca tccgatttgt tgatcattgc
     4381 gggccgcccg ggccagggta aaacggggtt cctgctttcg atcgcaaaga atgcggcgtt
     4441 gacccacaaa aagcatgtcg ccatcttttc gctggaaatg tcgaatgagc aggtggtgca
     4501 gcgcttgatc gcccaggaaa cggggatcga ttcgcaacgc ctgcgaaacg gtaaactgca
     4561 tgagcatgaa tggccgcttt tcacccatgc cattgaagtg ttttccgata cgcatatcta
     4621 cctcgacgat actcccgcca tcacccctct gcaattgcgc accaaatgcc gccgtttgca
     4681 catggagtat ggcattgacc ttgtcatcat tgactacctc cagctcatgg gcggcgactc
     4741 gcgcaacgat aaccgcgtgc aggaagtttc acacatctcg cgcagtttga aggtgctggc
     4801 tcgtgaattg aatgtccccg tgttgactgc ggcacagttg agccgtgccg tggaacaacg
     4861 cactgacaag aagcccgtgc tttcagacct gcgcgaatca ggctcacttg aacaggatgc
     4921 cgatatcgtc atgttcattt atcgtcccga ccaatacgaa aaagacagcg agaaacaaaa
     4981 cgtggctgag atcattgtgg ccaagcaccg caatggacct gttggcagcg tggaaatgat
     5041 cttccgcggc gcacaggcga agttcgaaaa tgcggccacg cgtgtattca gaccgaacga
     5101 gtgacaaggg aaaggatgat gggtgaattt tgaaggatga agatcgcgct catccttttt
     5161 ataaacccat aaagcaatgg ctgctttttc tggttttacc tcttctgaaa catttaccca
     5221 gatccctgat tcgttcatcc gcctgcttgg tgagatcgaa gatgtggctg aattgaaggt
     5281 ggctctccat gccatctggc ggatcgaaca tattgagggg aattttcggg ctttgcgtga
     5341 atcggatttt acggctgaga tgcttgggtt ggaaattaag gagatcaagc gcgggctgaa
     5401 gaaatgtctg gagcgcggca ccctgctcaa ggcggagaat gaatcggagg ttctgtattt
     5461 cctcaattcc ccgcgcgggc gtctcgcagt tgattctttt acaaaaggga aactggctgg
     5521 cattgcttcg gcaacacgtg aaagatcaaa cctgttcaag ttgtacgagg aaaacatcgg
     5581 cccgttaaca ccgttggttg ctgatgttct gaaagatgcg gaagaaattt atcaggccga
     5641 gtggatcgct gaagcgatcg gtctggcggt tgaaaataat aaaagaaact ggaaatatat
     5701 tgaagcgatt ttgaagcgct ggaaggaaga aggccgtgcc gaaaagcagg atagacgaaa
     5761 cgataaaaaa gatggccagc agagcctcag ccgaaagatc gagcagctcc gcaagcgcgc
     5821 cgataaaaaa tgatctgcct ggcgacccga actgcccgca ctgccacggt gtgggatatt
     5881 tgcgttcaga tgttcctgta gggcatgcgg aattcggcaa gctcgaaatt tgcacctgcc
     5941 gccgttccaa agtggctgat aatatccgcg accgtttgtt cgcgatgagt catcttgatg
     6001 agctaaaaga cctgaccttt gaatctttca aaccgcgcgg ccgcaaaggg ttgggagaga
     6061 tgcaggctcg ttcgattgaa atggcattca accacgccca gcattacgcc aaaagtttaa
     6121 atggctggct gcttttacag ggagggtacg gcagcggaaa gacccacctt gccgccgccg
     6181 ttgcaaattt tgttgttggc atgggcgttc ccactttgtt cctcacagtg cctgacctgt
     6241 tggatgcgct gcgttttgca tacggctccg aagatacaac ttttgaagaa cgctttgaac
     6301 agattcgctc tgcgaaattg ctggtgctcg atgattttgg aacgcaaaac gccacaggct
     6361 gggcacagga aaaattattt cagatcatca actatcgcta catcaataaa ctggccacag
     6421 tggtgacgac caatctctca ctggatgaga tcgaaccccg catccgctcg cgtctttcgg
     6481 acccggaact ggtcagccca gtgaaaatgt cggccccgga ttatcgccgc ccaatggaag
     6541 ataccagtca ccccgaactt tcctctttgg atctgctttc aagccgaacg ttcggttcct
     6601 ttgaagaccg tgccgcagag aaactcacgc cggatgagca taaatccctg caaaaggcgt
     6661 tgaagatcgc ccattccttt tctgaaaagc caaaaggctg gctggttttt gaaggcacct
     6721 acggatgcgg caagacgcac cttgctgctg ccattgccaa ttatcgtgct ggccttggtg
     6781 acccgccgtt gttcgtgatg gttcctgatt tgctcgatca tctccgtgcc gcttttagtc
     6841 ccaactcagg gacaagctac gaccgccgct tcgatgaagt gcgcaccgcc ggtctgcttg
     6901 ttttggatga ccttggtgcg caatctgcca caccttgggc gcgtgagaaa ttgcatcagc
     6961 ttttgaacta ccgctacaat gccgaattgc cgactgtcat cacagtggca aaggaaaatc
     7021 tcgatcaagg tcagatcgat gaacgcatca tcactcgtat gctggatgaa cgcctgtgcg
     7081 aatatatcga gatcaaagcg cctgcctatc aagggaaagg gaaacgcaag atcggcagga
     7141 agtagccttt ttaactgata tcaaggaaac gctgatgtaa cgctgaccaa agcggaccat
     7201 cagcgttttt tgtttaatgg gaggcggcac gggccgaatt tggagccttt tcctcaacca
     7261 aattttcaac atggttgaaa atttggttca agtgcctatc tagattctcc ccctggtctc
     7321 gtttataaac agggcaaata agaccaaaaa acacacaaag gaaaaagaat cgaatgctcc
     7381 cttcctattt gctctccctg cgcgaaggcc ttgaagccgc cctcattatc ggaattgtac
     7441 ttggcgcctt gcagaagatc cgccgtacag atctggctcc catgctttgg tatggcacat
     7501 ttgccgccat cggggttagc atccttaccg ccattttgct gaccattttt ggtttgagcc
     7561 tcgaaggcag cgccgagcaa atttatgaag gtatcaccat gttccttgcg gcaggcctgc
     7621 ttacctggat gatcttttgg atgagccagc aggctcgtac cctcaaagcc gaactggaag
     7681 ccgatgtgaa caaagccgcc gcatcacgca atggccgcgc cctgttttgg atggcttttg
     7741 tcgctgttgt gcgcgaaggc gtggaactgg ctttcttcat cactgccgcc ttttttgcgg
     7801 gcaataccga gaacgtcggc acgaatacga tccagacgct ggctggtgtg gtccttggct
     7861 tgagcacctc cattctgctt ggctggtcac tgtttgcaac cgctgtccgc cttgacctgc
     7921 gccgcttttt tcaggtcact gggttcatcc tcatcctttt tgcgggtggg ttggtggcgc
     7981 atggaattca tgaattcaac gaagtcggtt ggatccccaa tatcatcgaa catgtttggg
     8041 atgtgaatgc gatcgttgat gagaaatctg tgctgggtga attgcttaag actctttttg
     8101 ggtacaacgg caatccctct ctcaccgaaa tgatcggtta ttttgtgtac cttgctgttg
     8161 tggcggtctt cttccaacgc agtaatacca aaaaggatgt gatggtcgcc cagccttcgg
     8221 catagagtgg ttgggaaggt aactgatttt caagagcggg gttcgcgcac agcgcgaacc
     8281 ccgctctttg ttaataaagc tcttgacaac gaccacaaaa ataaatatac taagtgtata
     8341 atgaacacta agtatgaggc ttatgaacaa cccggtatca ccgttgacct tgccattttc
     8401 acggtcaaca acaacaaact caaggtaatg ctggtcaggc gtgccgaaga tccctataaa
     8461 gatttttggt ctttgcccgg cggattcatc aaacaaggcg aatcgctcga ggaagccgca
     8521 aacagggtct tgaatgaaaa gaccggtgtg aagaatgtgt acatggagca gctttacacc
     8581 tttggcgacc ccgcgcgcga cccgcgctca agagtgatca cagtgacata ctttgccctc
     8641 attccctggg ctcagcttgc acaacctgca tcggaaaaag ttgccgacct tgcctggtca
     8701 catatcgatc gcgtcccaaa actcgccttc gaccataaaa tgattttgga ttatgccgtc
     8761 aaacggctgc gcgcaaaagc aagttacagc aacatcgtct atgggttgat gcccgtgcaa
     8821 ttcaggcttt cagagctcca aaccatgtac gagatcatca tcaacgacaa actcgataaa
     8881 cggaatttca gaaaaagaat gctctctaca ggcctgctgc aagagaccgg cgaaaaagac
     8941 ctcaccggcg cgcacagacc cgccatgttg tatcaattca aaaaaatgga aatagctttt
     9001 ctcgattagg tgaaccatga aaaaacaaac cctgcccctt cctgaatttt ttgaatcggc
     9061 ccgtgtgggt gaaatctgga aagtggatta tgccagccgt gccgaacaag cccgtcaata
     9121 cgcgcgtcaa cacgacctga agcctgcctc tgctgcaagt gaaaagatct cccttttagt
     9181 gatcgatgca caaaacacct tctgcatgcc caattttgaa ttattcgttg gcggccgcag
     9241 cggacatggt gccgtggacg ataacgtccg cttgtgtgaa tttatctatc gcaaccttgg
     9301 ccgccttact cacatcatcg ccacgatgga tacccacatg tcacagcaga tctttcatcc
     9361 catctttttt gtggatcccg aaggcggtca ccctacccca tacaccgaca ttcatcttgc
     9421 agatttgata tcgggcaggt ggaaattcaa tgcagcactt gctccacgcc ttaatatcgc
     9481 ccccgagtac ggccaacaaa tgatgatcca ttacgctgaa tcgttggaga agaaaggcaa
     9541 atatgcgctt accatctggc cttatcacgc catgctcggc ggcatcggcc atgccttggt
     9601 ccccgcattg gaagaggcca tcttcttcca ttcgatcgca aggttcgccc agcctgattt
     9661 tgagatcaaa ggcaacaaac cgtttacaga gcattattcc gttattggcc ccgaagtgct
     9721 gactggcccc atgggcgaaa ccctcggctc tcacgataca acgttcatcc agcatttgca
     9781 ggaagtggat cgtttgtata tagcggggca ggccaagagt cattgcgtag cgtggacggt
     9841 ttcagacctg ctggctgata ttcaagacgc cgacccaagc ctggctgaaa aggtgtatct
     9901 gctcgaagat tgttcctccc ctgtggtcgt gcctggggtg gtggaccata ccgaagcggc
     9961 gaatgaagcg tacatccgct ttgcgcaggc tgggatgaag gttgtcaaat ccaccgatga
    10021 gatcagttca atgtaaacat acgggtgtat aaacagtaaa aatggagtat tatgccaaac
    10081 ctgacccgcc tctcaaagtt cgtgtctgtc attttgcgtc accgtgcgaa tgattttgac
    10141 cttcatctgg atgcgcaggg atttactgat tttgccgaat tgaaacgcat tgtgattgaa
    10201 aaatccaccg atgtttatag cgaagaagac tggcaaaagg ttttgaatgg cgagctcgat
    10261 ggaaaaaagc gtttcgaagt gaaagacgga cgcatccgcg cgatgtatgg ccatagcaag
    10321 gttgacccgg tcagctaccc gaaggttgag ccacccgaaa ttttgtatca cggtacaacc
    10381 ccgcaggctg aaaagaatat tcgcagggaa ggcctgcgtt ccatgaaacg tcagtatgtt
    10441 cacctgtctt caaatctgga gcgcgccaac tcggttggca gccgccgcac cgagcatgta
    10501 acgattttgg tggtgcgcgc cagagaggcc gccgttgcgg gagttgagtt ttatgcgcct
    10561 gagcctgagc attttttagc aagatccatc ccaccgaact ttattgattt cccacagaat
    10621 tgagaagccc catgtccatt tttgacggta agagactgac aaacgaaaca ttcaagctcg
    10681 atattgatcg catgcgccgc ggttggtatt cggataaata tttcgagaac atcaaccgca
    10741 tgttgaccgc actctcgaag gagaattaca actattccgg gagttttccc attttgccga
    10801 atggcatatc tcccgagcag atcgccgtgg gcgatatcga agtggagatg caatggttca
    10861 cacggcggat gggcaagacc accgtggtgg gtgtggataa gtctcttgaa atgctcaggc
    10921 attgtacggg ttatttcgat ggcgatactt ttgtggatac atctgatcgg ttggaggtat
    10981 gggcagtgca ggatggcagc attgtcaaat ttgaaggcga ccccacaaag attcaacctg
    11041 tcatcaaggt gcgcggacga taccgtgatt ttgccctgct cgaaacaccc acgctgggca
    11101 ttcttacacg ttccagccgt gtggcaacga atgtgtatga aaccctggtg gcttcacgcg
    11161 gcaagcctgt gctgttcttc cctgcgaggt tcgatgttca tgaagtgcag gctgcggatg
    11221 gatatgccta taacatcgcc ttgctgcgat ttaacaagga tcatgcccag gagttgggcg
    11281 cgttcgtttc gaccgatgcg cagggtgact ggtggggggg tgctggcggc gggacagtgg
    11341 cccatgccgc gattgcatcc ttccttggtg atactgccga ggcgatgatg cagtttgcac
    11401 acatcctccc tgccagaatt ccgcgcattg cactggtgga tttcaacaat gactctgtcc
    11461 gcgattcgct gcgggtgttg gatgcgatgt tcgctaaata tcgcgagttg atggatgcag
    11521 gcaacaggga ggaagcggaa aagtacaagc tttacggtgt gcgccttgat accagcggaa
    11581 gtttacgtga tgtatcggtg cagcctttgg gcgaccccgc tttggatctt ggggttaacc
    11641 cgcggctggt gtttaatgtc cgccaggggt tggataatgc ctgggaaagc tggaccctgc
    11701 ccacgtcgtg gaaggatgcg gcgcgggaat tttgtcataa tgtgaaaatc gttgtttcgg
    11761 gggggttcaa tccagaaaag atccgtaaat ttgaaaggct tgaagtgcct gtggatattt
    11821 atgctgttgg ttcgtatctt ttcaataaca gcaatagcac atccaccgat tacacggcag
    11881 atgtggtgcg cgtgaagatt catggagagt ggatcgaaat ggcgaagatg ggacgcaagg
    11941 tcggcgaaaa cgaaaacctt gaacgggttt ggtgattgag tggttgatca aattaaaaaa
    12001 ggctcatgaa aaacgttgcc aaagcgtgtg cctgacgtag gggttgcgtc cagtattttt
    12061 ttgaggtgag tgataaagtt gaattctctt ctcctctgga aaatgaaatg gccggtgaac
    12121 atcaccggcc atttcatttt tgtaacgata gactgttttt tgaatgagcg ttagttggct
    12181 gggatcaatt gctcggcgat cttgagtaat tcgtccatag gtaaattggt gagtagctca
    12241 atttttgtgc cgcccatgat gaaggtcagt ttggaggcat tgaagtattc aaaagattct
    12301 ggtgcaatcg gggcaaggtt ttcaacagtg acatccacat tgggttgcga ttgcagtgac
    12361 aggctttcag agtcaaggtt gtcaatgatg atatctccat ctgcttgcga ttgttccagt
    12421 ggaacttctg ctccgtctgg cagtgtgcca gtatagattt gcagatcctg tgatgatgag
    12481 gttatttcca gtgatgcctc gggggtcata tctccgccgc tgctgaggta aatgccgcca
    12541 ctgctgttgg tgtcactgct ggggggctga ttttgatatg ttccagacag gtttgtgttt
    12601 agtacggcag gcagaccgtt gatcgtgaca gcttctccat ctggcagtgc atcgttgtcg
    12661 gcggcgggtt gttgggtgat gattaagaac gtatcatcca ctttgtagag gagctctgaa
    12721 acatcgccaa agcttgcaga gtttatatct ttgaagatct caggcaggta tccaggctgc
    12781 attaactgat atttcattgg ctcgttgctg acgaagccaa aactcaagtt gctgttgttg
    12841 gggctttgaa atttgacaaa aaaccattcc tcgacttgtg cccgcaccgg ggggacaaag
    12901 acaacggtgg cggtcagtgc aagaagcatg gccaatgtca aaaatgcata tttgtatttc
    12961 atggttgtag tttttcctgt tctcttaacc tgccagggag cggttttcat ttttgaataa
    13021 aaaccctcgc caggcatggg ctgaattgtg gataatattt tttcgatctg atgttcatcg
    13081 ggcatggatt tattttgagg cattttcatt ctcctacgac tgaggccaac cctgctgtaa
    13141 acggttcaag gcacgacgca tactgacgct gacattgttt tcggttatat cgaggtgtaa
    13201 tgcaatctgc tttacctgcc agccaagcat atagcgcaag acgagcattt cgcgcaggtc
    13261 ctcgggcaaa gtggataatg tcttccatag gaccatagct tgttcacggg tgatgatgtc
    13321 gatttcgggc agcgatttcg tgtccagcag tgattcgagg tcgatgtttt cttcttcggg
    13381 gcggaccctg cgtcggcgtg acgaatcaat gatcaggttg cgtgcgattc gtaaaatcca
    13441 accgagtgcg gcctgtttgc tgccagtgaa gcggtggcgg gtggtccatg cccggatgaa
    13501 ggtttcggct gtaatatcct ctgcgtcttg ctgaggtccg ccgcttagcc catatacata
    13561 gcgaaatacg gtcaggtggt gtagttcata gagcgttgca aaggcatcag catccgtgat
    13621 ggaatattcc tgttcttgcc ataaccagcg tttaatgttt tgcagtacgg gttgttcagt
    13681 caaacagacc tccgatatgt gaaatacgcg cgttcaccat ataaaacgca gatgggctca
    13741 aaatctgaca aacgaatata cactggaata attaaaggct tatggaattt tgagtattgt
    13801 gggggatgtg tcagtctgtt ttggggtggg tgacatggtc aaggccgttt cttttttctg
    13861 gaaagtggtt ggcccaagcc ttgtttattg tgattttttg cattaataac tggatgacag
    13921 tataatcaac aaaaatacat tgctgtgagg tggattgtga aacgaatttt catttcttat
    13981 cgcagacagg atagcggggt ctttattgga aggatttatg atcaacttaa actccatttt
    14041 ggcgaacgca atgtgtttcg ggatgtatac aatattcccg ctggcagtga tttccgtgaa
    14101 gtattgaata aggaagtggg gcagtgtgac attttcctgg ctgtgatcgg acgccagtgg
    14161 gtggatatct ccagtactca gggcaggcgg cgtttggatg accccaatga ttttgtgcgt
    14221 attgaagttg aattggcatt aagtaatcca aatatccttg tgatccctgt tttggtggac
    14281 aatgctgaaa tgcctgaggt tgaagatttg cctccgagct tgcagccttt gtgttatcgg
    14341 aatgcggtca aagtgcggac agatcccgat ttcccccatg acatgcaaat gttgttgcaa
    14401 caaataaagc ccgccaagtc tttcatatca acccgggttg gctggttgat gttggccttg
    14461 tccatcctcc tgatcgcggg attaagtttt ttccccatgt tcaatggaga aaaggacatt
    14521 ccatctccca cagctgcaca gactgatata ccgccgacac ccactccgct gtttgctccg
    14581 gttgaacctg tggagcctgg tcaggttatg gtccttgtgg ctaacatgga gcaggtcggt
    14641 tcaacaaagc gggatgtgac ccgttttatt gtagaagatc ttgtcaatca ttttgagtct
    14701 gatctgtcga gtacgaatat ccgtattcgg gagtatgaac gatttattac ctccaatgat
    14761 gaagcgctgc aattggccga gcagacacaa gcggtgattg tggtttgggg acagtatgat
    14821 aacgacaatg ttacggtgaa tattcaactt ggttccctgc agtcccgacc tgccatagca
    14881 cttgaccgtt ccctgcttga gcaggccgtc aatattcgag tgaaactgac ggatgaacgt
    14941 caggagaccc tggcatatca catcttgagt tctcttatcc ttatccattg tgctgaaaat
    15001 gacatggtca aagcgatgag tttgatcatt tcgtttgacc ttctggatgc gcccggcccc
    15061 gagcctatcg ggaattcgag tgctgcccat gtgcaaaagg caatgaaggc atttctttcg
    15121 gatcgaaaaa cagcgttgga tgagctgaat cagacggttg agcttgctcc tgataatcca
    15181 ctgttttaca tgcttcgcgc aatactgtat caaggcatgg gggaatttgc cctaagtaat
    15241 caagatcttg ataccgcact gttgctggct cctgaagatt ggattctgcc ttaccatctt
    15301 aaaggcggaa taagccttct tttgagcgat gttccaaatg gcgtggagtt ttatacaagg
    15361 gtgatcgaga aaactcctga tagctggttc ccttataacc aacgtggcta cttgtatttc
    15421 cttgcaggac agtatgatct ggcgcgtagt gatattgaga aatccattgc gctcaaaccg
    15481 gaggcagaat ggccgtatat gtggggcgca ttgattgctt tgcgtcaggg gcgcatggaa
    15541 gatgcctcct ttatgatgtc actcctgttg caggaacagc caaacccaac ctttattaat
    15601 cgtttgatga ccattttata tggggaagcc actgcaaaac tgttgggcga atcgatggct
    15661 gcaatgggac atctcacttt gggccagtat gatgtggcta ttcaagatac ggacggcgtt
    15721 ttggcggtca tgcctgccta tactgatatg tatatgttgc gcggattaag ctattgcaac
    15781 ctggataact atgtggatgc agaggccgct tatacacagg gcctgaccct tgacccctca
    15841 tttgcccttt tacatttgct gcgtgtggag gtccgaacaa aacaggggaa tgtagcgggc
    15901 gcaatggagg atattgatgc cgtacaacag aataatgaac tcacagctaa tttggctccc
    15961 tatcttgctg cggctcagtc tggggagttt tcttgtaaac aattattgag atccaaatag
    16021 gcgtaaaggt tttttgtttt acgaaaaaaa ggtcaagata tccagtacgg atatcttgac
    16081 ctttaaaatt cagcagtttc cttttgggcg ggtgagtggt tattactttt gggccatgtt
    16141 catgcaaaat attgaatgat attttctgca atatgaacga tcaactatcc aaaaacgtga
    16201 ttaaaaaaat taaggcagat tacaaattgg aagaaattcc ccgtgtggcc gaatgtttag
    16261 gacaatacgg cactgacaaa cccgagacag aacgtgtgca gcttgggatc ttaaacgtgg
    16321 caaatgggga tattcccaaa ctccaaatgt tggtgtccgt tgctcttacc gattacaggg
    16381 acctgctcgt gcaggcggaa taccttaatg atccttataa tcttttgcaa accctgcttc
    16441 gaaacttgca agagagcaat acatttacaa cagaggaagt gacggaaacg atgcgcgcca
    16501 cgggtggaac gaactatcga aaggcttttg agttccttat ggtaatgatc ccccagcgcg
    16561 ggaagaccct gaacgctgag caatatcaat taatagataa actcggcagg gcgttgaaac
    16621 tgcccgtcaa aacatggaaa catcttgctg tataagaaac aaaactccac aagcatcaac
    16681 ttgtggagtt ttgtttctac ctagaaaaca ttcctgcctc tgaattaaat tttatggctg
    16741 agaccagatt agggaattgc gtttgtggca gaatttgcgc agtggatgtc cagtcatgtc
    16801 cgttaacttc cctgttcttt tctttaaaaa catcttctct ggtgatcatt tgaaaatcgg
    16861 ccagaataag ggaggggggt ctcaactgcc ggtggcaccc ccaaagcgac ccgtaccacc
    16921 gatcacagtc cactgatcgg ttccaacgaa cacaccaggg ctggtctgca cggaacctga
    16981 ttgctcatgg ccaatgcagg gtttgcacct gctccacggg cgaccagggc aatgatcttc
    17041 gctatagcca cacttccctg cc
//
LOCUS       DAOQLC010000096        16955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1215_length_16955_cov_31.561479, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000096 DAOQLC010000000
VERSION     DAOQLC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16955
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1215_length_16955_cov_31.561479"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1447
                     /locus_tag="PL000_15375"
     CDS             32..1447
                     /locus_tag="PL000_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNC90319.1"
                     /translation="MTTGLPESKAKRVFNNVLGAMGNTPLVKLERIGRDLPVPLYAKL
                     EFMNPGGSVKDRVGANIIEQAEKRGEIKPGGTIVEATSGNTGVGLAIAAALKGYKTIF
                     VMPDKMSNEKILLLRAYGAKVVITPTAVGPDDPRSYYEVAKKFARETPNAILANQYHN
                     PDNPKTHELSTGPEIWEQTDGKLTDVIIGIGTGGTITGVGRYLKSKNPNITIVGVDIE
                     GSILTEIWQNKGIIPPGAYPKTYKVEGIGEDFLPSTMDINYVDAIERAGDKESFYWAR
                     QLVRQEGIFAGGSSGSAIAGALKYCRKLTPDRLPVVILPDSGSRYLSKFYDDKWMREF
                     GFLSMEFGETALGDLLIAKPNKQLITATLGDSIRKVVSVMHQNAVSQMPVLGADGSLV
                     GLIEEVDLLNHMLDEHDHSNDEKIDALIQNAGAVFPPETPLEDAMPSLTSGYALIVVD
                     HGKPVGILTKIDVLDYVAGKI"
     gene            1503..1973
                     /locus_tag="PL000_15380"
     CDS             1503..1973
                     /locus_tag="PL000_15380"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC90320.1"
                     /translation="MMPFLLRIWRTLPLWVHFLAAKLVRPRFRAAVAALIFNELGHVL
                     LFKHTYRKFEWGIPAGGLEYREQPADAVRREFFEEAGMHIQVERLLTVVSAREDHHLG
                     VVYLCKVTEGEFRPSLEISEMKYFPVDDLPTMMLTTEKELIKWAVENQATDKLR"
     gene            1996..2379
                     /gene="crcB"
                     /locus_tag="PL000_15385"
     CDS             1996..2379
                     /gene="crcB"
                     /locus_tag="PL000_15385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNC90321.1"
                     /translation="MTNLLLVGFGGFIGSILRYLASGYVQQISKGMGFPYGTLTVNVV
                     GCFIIGFLAQLAETHGVFTTESRLFVFVGILGGFTTFSSFGNETFNLARDGQMMGALA
                     NIGANVIVGLLAVAAGRIVSSLIWG"
     gene            2511..3425
                     /gene="prmA"
                     /locus_tag="PL000_15390"
     CDS             2511..3425
                     /gene="prmA"
                     /locus_tag="PL000_15390"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNC90322.1"
                     /translation="MNWLEVSLTVDGELAESVADVLARFAPNGVMTEQGVKFNNEEDE
                     GTATGPITVRAYLEVNDQLEETRQKLEESLYYLGMIQPVPTPAYKQIADQNWMEAWKQ
                     HYKPILIGKSLLILPAWLESPEPSRIPIKIDPGMAFGTGTHPTTQLCLELMERSSLVT
                     SPLSQVIDVGCGSGILSIAALKLGAEKVLGVDIDIESVRNSRENADTNGVGDELILGQ
                     GSVTEVLAGQFAFKSAPLVVANILGPILIRLFDAGLADLVEPNGEIILSGILDHQAES
                     VIGAGQAKGLKRGEVRQMGDWVAISMSR"
     gene            3510..4274
                     /locus_tag="PL000_15395"
     CDS             3510..4274
                     /locus_tag="PL000_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90323.1"
                     /translation="MKRANNFLLAVTCLALAAMACSAVSGGAEPTPITIPDIPTLPSF
                     DATAIPDVPQPTDVPPSNGGGGDVLFQDDFVPGRGEWGTGSDADRTVEYVNEQLNIKI
                     AAERYFVWTTPNDQDYNKVHMEVTAYNHNADPNVGFGIICNQQFIEDSMYYFAITSNG
                     EYAIVRAALAQDDVFLTNNGQWNTSDLIAKNAESYRIGVDCANGKLALYVDGQLIDSV
                     DDSTYAAGGFALFVWSADQANGADVAFDDFIMTQLP"
     gene            4367..4858
                     /locus_tag="PL000_15400"
     CDS             4367..4858
                     /locus_tag="PL000_15400"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNC90324.1"
                     /translation="MNRRKMFLYILLNIFVSASVTGGMLFWYDRNYRAVSQSVVAQVT
                     PVSGEVDSASETDLQTDIPVKISSVVGAGTVTSEIVVVKFEGEGQLDLASWQLKDEDG
                     NTFTFPKLTLYQNGAVQVHTIAGTDTVIDLYWGINDAVWNSGENARLFDAQGNLRAVY
                     RVP"
     assembly_gap    4872..4971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4972..6550
                     /gene="dnaX"
                     /locus_tag="PL000_15405"
     CDS             <4972..6550
                     /gene="dnaX"
                     /locus_tag="PL000_15405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC90325.1"
                     /translation="HMTQALYRKYRPKQWDTVIGQDHVVQTLINSIKADRVGHAYLFA
                     GPRGTGKTTVARLLAKAVNCLNEDPAKRPCNECDHCKAVNENRFMDMIEIDAASNNGV
                     DDVRDLREKINFSPSQGRYKVYVIDEVHMLSGGAFNALLKTLEEPPPHAIFVLATTEI
                     HKIPATVLSRCQRHEFRRVPIDEIVANLKQIASSEHIQVDDEAFALIARQSAGGMRDA
                     QSLLDQLASTGTKITLALAQQVLGTATSQTVLDVATSILENQPAHGLETIHRALDSGA
                     DPRSLARQIVEYMRGLMLIQMGNIEQVEATRDVKTQMQAHANAFHSTDVLRMMKIFNN
                     AAVDLRGGWQPSLSLELALAEALDAPTESPQITAAPRRTPPKVDSVSKTESQPEVKSA
                     SSPQGEASPVQSESAPEKPILNSNDIVRSWKSLAASLPKSQANLSALLNSVKMIDIQG
                     KTLLLGLASDVLVSKLDKPDQIEAIQKLIKDHFHVDVAVKCIVTNAKGKIPPNVAQDG
                     MVAAAIHHGGEIVDMDD"
     gene            6605..6934
                     /locus_tag="PL000_15410"
     CDS             6605..6934
                     /locus_tag="PL000_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNC90326.1"
                     /translation="MAKGFNRGPAMGGQSGMLQQLQQMQKKMEIAQAQLAEETVSATA
                     GGGAIKVVMTGDQKCKSVEISPEFLKDADAEMLQDMVLSAVNMALDESRALQEKLMGP
                     LAGGLPF"
     gene            7005..7610
                     /gene="recR"
                     /locus_tag="PL000_15415"
     CDS             7005..7610
                     /gene="recR"
                     /locus_tag="PL000_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNC90327.1"
                     /translation="MILPESIQSLVTALERLPGIGPKSASRLAFYLLRASEDVAQDLS
                     VALANLKANTAFCQECFNITDAGRERCEVCESTNRDGSLICVVEEALDVLALERTGGF
                     HGKYHVLQGVLSPIEGIGPDDLKIKQLVARVANGGVKEIILATNPSMEGDATAQYLQS
                     QLRAYNVHVTRLARGLPVGGDLEYADQNTLLRALSGRQEMN"
     assembly_gap    7611..7710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7992..9167)
                     /locus_tag="PL000_15420"
     CDS             complement(7992..9167)
                     /locus_tag="PL000_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNC90328.1"
                     /translation="MEENLAGKTCFVIMPFGEKKDLDGQPINFDLIYRDFIKDAVEGL
                     DLKCIRCDEIAEAGWIHSKMFEYIFSADVAVVDITSLNPNVFYELGVRHALKKSVTIL
                     IRKSGTPTPFNIQGFQMLEYKPDDLQNLENGKQKIRDFIKNGIAGQVLDSPIYEVLDN
                     LKVERKPKRIGAKNIHLYPITKVAGKEIGMITGDIQNIKEIDVWVNSENTNMQMARHF
                     ERSISATIRYLGAKRDRAGRVVDDLIANELREAAGTADVPAGIVIPTGSGELNKSHNV
                     KKIFHAASVIGQVGRGYMPITEIAECVRNALELIDSEEMAAENIQSILFPLMGTGTTR
                     LDADEISRQLIDAAISYVEENPQTKVQKIFFLAYHEEDLNICRHILEHDARITHPKS"
     gene            complement(9181..9771)
                     /locus_tag="PL000_15425"
     CDS             complement(9181..9771)
                     /locus_tag="PL000_15425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC90329.1"
                     /translation="MKKVVITQKALALDDFFQVEEAHLQYELFNGEMSRPVRRFSLER
                     GNSAAVLVYNLDTQKIILINQFRYPTYKNGHGWLTEIIAGMVDPGEKPEESARRELLE
                     EAGLVVSTFETICSFYPSPGGSSEMVFLYYAEVSGEFALYNKTGGLPGHGEDIISLEM
                     TLDNALEDIREGKIVDAKTIMAIVWLENRMLKKSHS"
     gene            9946..11193
                     /locus_tag="PL000_15430"
     CDS             9946..11193
                     /locus_tag="PL000_15430"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC90330.1"
                     /translation="MSLPVYRRKFQTFISSVNASNEVAEQIEHWLTDKAGIPVLHDIQ
                     NFQSGASMEDGLELCQSMLVLITKRGIESGRVKKEFELGVKQQARFGNFFHVIPVRVE
                     ECIIPDFLEHSAFVDTVESGFDMSAAMKLLSGLYSNGLVSGDGKTHDLFVSCPWSREE
                     DALTKLVFKLAKRIGFRLIGNPIETHEKSDVSTSLISGCGGGIAVIPDGIGEKTMNKI
                     ISDLGIMSDLNLPFLVVMEKVVNLPDLITQHALEIVHLDKDTLSQDADLEKQIRASVI
                     RLYEDWVSPLEQHYIFYGTDLKEEHKARSRFVRRAIQQITSMPCMMGEDIQRGQIQNE
                     IINMIIGARMMIADISRENLNTCIEAGVAIGSNVPLNLLSGDERHKPPFMFRDRQIWH
                     YQNELELLGMVHKLVAPYRKFVI"
     gene            11702..13211
                     /locus_tag="PL000_15435"
     rRNA            11702..13211
                     /locus_tag="PL000_15435"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            13334..13407
                     /locus_tag="PL000_15440"
     tRNA            13334..13407
                     /locus_tag="PL000_15440"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13368..13370,aa:Ile,seq:gat)
     gene            13424..13495
                     /locus_tag="PL000_15445"
     tRNA            13424..13495
                     /locus_tag="PL000_15445"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13456..13458,aa:Ala,seq:tgc)
     gene            13802..16752
                     /locus_tag="PL000_15450"
     rRNA            13802..16752
                     /locus_tag="PL000_15450"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            16832..16948
                     /gene="rrf"
                     /locus_tag="PL000_15455"
     rRNA            16832..16948
                     /gene="rrf"
                     /locus_tag="PL000_15455"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         4305 a         3885 c         4485 g         4080 t
ORIGIN      
        1 cggtaaaata cccacaacca aaggagagag aatgacaaca ggattacccg aatcaaaagc
       61 gaaacgtgtc tttaataacg tacttggcgc aatgggcaat acgccgcttg tcaaactgga
      121 gcggatcggg cgcgacctgc ctgttccact gtatgcgaaa ttggaattta tgaacccagg
      181 cggttcggtg aaagacaggg tgggggcaaa catcattgaa caagccgaga aacgtggcga
      241 gatcaaaccg ggcgggacca ttgtcgaggc cacatcgggt aatactggcg ttggccttgc
      301 cattgccgct gcgctcaaag gctacaaaac catcttcgtc atgcctgaca agatgagcaa
      361 tgaaaagatt ttattgttgc gggcgtatgg cgcaaaggtg gtcatcaccc caactgcggt
      421 cggtcccgat gatccgcgct cgtattatga agtggcaaag aagttcgcgc gagaaacccc
      481 caatgccatt ctggccaatc aatatcacaa ccccgataac ccgaaaaccc acgagctgag
      541 caccggccct gagatctggg agcagaccga tggcaagctg accgatgtca tcatcggcat
      601 tggcacaggc ggaaccatta ccggtgtggg gcgttacctc aaatctaaga atccgaacat
      661 tacgatcgtt ggcgtggata tcgaaggctc gatcctcact gagatctggc agaataaagg
      721 catcattcct ccgggcgcgt atcccaaaac ctacaaagtg gaaggaatcg gcgaagactt
      781 tttaccctct acaatggata tcaactatgt ggatgccatc gaacgcgcag gagataaaga
      841 gtcgttttat tgggcgcgtc aactggtgcg gcaggagggc atttttgcgg gcggttcctc
      901 tggctccgcg attgcaggcg cgctcaagta ttgccgcaag ttgacccccg atcggctgcc
      961 tgtggtcatt ctccctgatt ctggttcgcg ttatctttcg aaattttacg atgacaagtg
     1021 gatgcgcgaa ttcggtttcc tatcaatgga atttggagaa acggctctgg gtgacctgtt
     1081 gatcgccaag cctaacaaac agttgatcac cgcaacatta ggcgactcga tccgcaaggt
     1141 ggtttcggta atgcaccaga atgcagtatc gcaaatgcca gttctcggcg ccgatggatc
     1201 gctggtcggc ctcatcgaag aagtggatct gctcaatcac atgctcgacg aacacgacca
     1261 tagcaacgac gaaaagatcg atgcgctcat acaaaatgcg ggcgcggtct tcccgcccga
     1321 aactccgctt gaagatgcca tgccctcgct cacatcaggc tatgcattga tcgtggtgga
     1381 ccacggcaag ccagtcggca tcctcacgaa gatcgatgtg ttggattacg tggcaggcaa
     1441 gatttaggac gatttgaacg cccagttgag agataaaact ttcaactggg cgattgaaac
     1501 ttatgatgcc tttcctgtta cgaatctggc gaactttgcc tttgtgggtg cattttctgg
     1561 ctgcgaaact ggtgcgtccc agattccgag cggcggtggc ggcgctgatc ttcaatgagt
     1621 tgggacatgt actgctgttt aaacacacct atcgcaaatt tgagtggggc atccctgcag
     1681 gtggtttgga gtatcgtgaa caacccgctg atgcggtcag gcgtgagttt tttgaagaag
     1741 cagggatgca tatccaggtg gaacgcttat tgaccgtggt cagtgcgcgc gaagatcacc
     1801 atttgggtgt ggtgtacctg tgcaaggtca cagaagggga attcagaccg tcattggaaa
     1861 tttcagagat gaaatatttt cccgttgatg atttacccac gatgatgctc accacagaaa
     1921 aggaattgat aaaatgggcg gtggaaaatc aagccacgga taaactcaga tgaacatagg
     1981 aacaaaggaa atcagttgac caatcttctc ctcgtcggtt ttggcggttt tatcggctcg
     2041 atcctgcgtt atcttgcaag cggatacgtt caacagattt cgaagggcat gggctttccc
     2101 tatggcacac tgaccgtcaa tgtggtcggc tgttttatca tcggctttct ggcccaactt
     2161 gccgagacac atggcgtatt tacgactgag tcacggcttt ttgtttttgt tggcattctt
     2221 ggcggattca ccacattttc ctcgtttggt aacgaaacgt tcaatcttgc ccgtgatgga
     2281 cagatgatgg gtgcgctggc aaacatcgga gcaaatgtga ttgtgggact tctggctgtg
     2341 gcggctggca gaatcgtttc atccttgatc tggggttgat ggaacaactc aaaatgcggc
     2401 atgtgttccg tagccaaaaa acggctgctt tggcggccgt ttttttatct ctgacagttt
     2461 tggtcgtttg gttgtaaaat tcggcaccta ttattttccc tggagtaaat gtgaactggc
     2521 ttgaagtatc cctcactgta gatggcgaac tcgccgaatc tgttgccgat gtgttggcgc
     2581 gctttgcacc caacggtgtg atgaccgaac agggtgtcaa attcaacaac gaagaggacg
     2641 aaggcacagc cacaggtccc atcacggtcc gtgcttattt ggaagtgaac gatcagcttg
     2701 aagagacgcg ccaaaaactg gaagagtcgc tttactattt ggggatgatc cagcccgtgc
     2761 cgactcctgc ctacaagcag atcgcggatc aaaactggat ggaagcctgg aaacagcact
     2821 ataagccgat tctcatcgga aagagtttgc tgatcctgcc tgcctggttg gaatccccgg
     2881 agccgagccg tattcccatc aagatcgatc ctggcatggc atttggaaca ggtacgcacc
     2941 ccacgacaca gttgtgcctg gagttgatgg agcgctcatc actcgttaca agtcccttgt
     3001 cgcaggtcat tgatgttggc tgcggttctg ggatactttc gattgccgca ttgaaacttg
     3061 gcgcggaaaa ggtcttgggt gtggatattg acattgaatc ggttcgcaac tctcgtgaga
     3121 atgccgatac aaatggagtg ggggatgaat taatcctcgg gcagggttca gtgacggaag
     3181 tgctggcggg gcagttcgct ttcaaatcgg cgccgttggt ggtggcgaat atcctggggc
     3241 cgattttgat tcgccttttt gatgcagggc tggctgatct ggtggagcca aacggcgaga
     3301 tcattttaag tggcattctc gaccatcagg ctgaatctgt cattggggct gggcaggcca
     3361 aagggttgaa aagaggcgag gtgcgccaaa tgggtgattg ggttgcgatc tccatgtctc
     3421 gttaaatcca cacattcccc ataatgtact atttagttat tggcacagac tgataaaatc
     3481 ccgccatcat aattttagga gaatgtatca tgaaacgtgc caataatttt cttcttgctg
     3541 taacctgtct tgcgcttgcg gccatggcct gtagcgcggt ttcaggtggc gcagaaccca
     3601 ccccgatcac catccccgac atcccaaccc tcccgtcttt tgatgccaca gccattcccg
     3661 atgtgccgca gccgaccgat gtgccgcctt caaatggtgg tggcggtgat gttttgttcc
     3721 aggacgattt cgtccctggc cgtggcgagt ggggtactgg ctccgatgcc gaccgcaccg
     3781 tggaatatgt gaatgagcag ttgaacatca agatcgctgc tgaacgctat ttcgtgtgga
     3841 ccacacccaa cgatcaggat tacaacaagg ttcacatgga agtgactgcc tacaaccaca
     3901 atgccgatcc gaatgtgggc tttggcatca tctgcaacca gcaattcatc gaagattcaa
     3961 tgtactactt tgcgattacc tcgaatggcg aatatgccat tgtccgtgcg gctttggcgc
     4021 aggatgatgt cttcctgacc aacaacggtc agtggaacac ctcagacctg atcgccaaga
     4081 acgcagagtc ctatcgcatc ggcgtggatt gtgccaacgg taaattggcc ctgtatgtgg
     4141 acggccagtt gatcgactcg gtggacgatt ccacctacgc tgcgggcggc tttgctctgt
     4201 tcgtgtggag cgccgatcag gccaacggtg cggatgtggc ctttgatgat ttcatcatga
     4261 cccaactgcc ctaaaacggt tttgattttc aaagtcctgt ggtcggtaga tcacaggact
     4321 tttttatttt ccgcctgcgc acattctgtg ctatgattca agcaccatga accgccgtaa
     4381 aatgtttttg tacatcctcc tcaatatctt cgtctcagca agtgtgacgg gggggatgct
     4441 gttctggtat gaccgcaact accgcgctgt cagtcagtct gttgtggcgc aggtgacacc
     4501 cgttagcggc gaggtggatt ctgcctcaga aacagacctg caaacggata ttcctgtgaa
     4561 aatcagcagt gttgtgggcg caggcacggt cacctctgag atcgttgtcg tcaaatttga
     4621 aggcgagggc cagttggacc tggcctcctg gcaattgaaa gacgaagacg gaaacacctt
     4681 caccttcccc aaactcaccc tttatcaaaa tggagccgtg caggttcaca ccattgctgg
     4741 tacagatacc gtcattgacc tgtactgggg catcaacgac gctgtatgga attctgggga
     4801 aaatgcccgc ctattcgatg cacagggcaa tttgagggct gtgtaccgag ttccgtaata
     4861 ttcacaaaaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacacatgac
     4981 ccaagctctg tacagaaaat accgcccaaa acaatgggat acagtcatcg gacaggacca
     5041 cgttgtccag acactcatca attccatcaa ggctgaccgc gtaggtcatg cctacctttt
     5101 cgcaggtcca cgcggtacag ggaagactac tgtggcgcgt ttgctggcaa aggcggtcaa
     5161 ctgtctcaac gaagaccctg caaaaaggcc ctgcaacgaa tgcgaccatt gcaaagctgt
     5221 caacgaaaac cgctttatgg acatgatcga aattgacgcc gcctcgaaca atggcgtgga
     5281 tgatgtccgt gatctgcgcg agaagatcaa tttttcacct tcgcagggca gatacaaagt
     5341 ttatgtgatc gatgaagtac acatgctttc tggcggcgcg ttcaatgcct tgctcaaaac
     5401 gcttgaagaa ccgcccccgc acgccatttt cgttttggcg accactgaaa ttcacaagat
     5461 ccctgccacg gttctgtcgc gatgtcagcg ccatgaattt cggcgtgtgc ccattgatga
     5521 gatcgtcgca aacctgaagc agatcgcctc ttctgaacat attcaagtgg atgatgaagc
     5581 ctttgccttg atcgcccgtc aatcagcggg tgggatgcgc gatgcgcaat ccctgcttga
     5641 ccagctcgca tctacgggca caaagatcac gctggctctt gcgcagcagg tccttggcac
     5701 ggccacgagt caaaccgtcc ttgatgttgc cacatccatt cttgaaaacc agcctgccca
     5761 tgggcttgaa accattcacc gcgccctcga ttctggagca gatccgcgct cgctggcccg
     5821 ccaaattgtc gaatacatgc gtggcctcat gctcattcaa atgggcaaca tcgagcaggt
     5881 cgaagccaca cgcgatgtca aaactcaaat gcaggcccat gcaaacgcat ttcactccac
     5941 cgatgtgctt cgcatgatga agatcttcaa caacgcggca gtggatttgc gcggcggctg
     6001 gcaaccctct ttgagcctgg aacttgccct cgcagaagca ctggacgccc cgacagaatc
     6061 tcctcaaatc accgccgcgc cgcgtcgcac cccgccaaag gttgattctg tttctaaaac
     6121 agaatcccag cccgaggtta agagcgcatc gtccccgcag ggggaagcgt cgcccgtcca
     6181 aagtgaatct gctcctgaga aaccgattct taattccaac gacattgtca gatcgtggaa
     6241 gtcgttggcg gcgtcgcttc caaaatcgca ggcgaattta tcggcattgt tgaattcggt
     6301 gaagatgatc gatatccaag gcaaaacgct cctgctcggc ctcgccagtg atgtgcttgt
     6361 ttccaagctc gataagcccg accagatcga agcgatccaa aaattgatca aagaccactt
     6421 ccacgtggat gttgcggtga aatgcattgt caccaacgcc aaaggtaaaa ttcctccgaa
     6481 cgtggcgcag gatggcatgg tggctgccgc cattcaccac ggcggcgaga tcgtggatat
     6541 ggatgattag gaatatctga tgcttgcatg gtgagcatcg aataaaaact tacgaggttt
     6601 aattatggct aaaggattca atcgtgggcc cgcaatgggc ggacaatcag gcatgttgca
     6661 gcagttacag cagatgcaaa agaaaatgga aattgcacag gcgcagcttg ctgaagaaac
     6721 ggtcagcgca acggctggcg gtggtgcaat taaagttgtg atgacgggcg accagaaatg
     6781 caaatccgtt gaaatttccc ctgaatttct caaagacgca gatgccgaaa tgcttcaaga
     6841 catggttctc tccgcggtca acatggcgtt ggacgaatcg cgcgccttgc aagagaagtt
     6901 gatgggtccg cttgcgggtg gtttgccttt ttagggtgca agttacaggt tgaaagttgc
     6961 aggattgttt ttcaatctgt aaccttctac cttcaacctt caacatgatc cttcccgaat
     7021 cgatccaaag tttagtcacc gccctcgaac gcctgccagg cattgggcca aagtctgctt
     7081 cacggctggc tttctatttg ttgcgtgcat ccgaagatgt ggcgcaggat ttgtctgtgg
     7141 cgttggcaaa cctcaaagcg aataccgctt tttgccagga atgtttcaac attaccgatg
     7201 caggccgtga gcgctgcgaa gtgtgcgagt cgaccaatcg tgatggctcg ctcatctgtg
     7261 tagtggaaga agcgttggat gtgcttgccc tcgaacgcac aggcggtttt catggaaaat
     7321 atcatgtctt gcagggagtt ttatctccca tcgaaggcat tggccccgat gatttgaaga
     7381 tcaagcaatt ggtggcccgt gtggcgaatg ggggagtgaa agagattatc cttgccacca
     7441 atcccagcat ggaaggcgat gcaacggcgc aatatctgca aagtcaattg cgggcgtaca
     7501 atgtgcatgt cacacggctg gcacgcggtt tgcccgttgg cggcgatctc gaatatgccg
     7561 atcaaaatac cctgttgaga gcattatctg gaagacagga aatgaattaa nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgccgatcaa aataccctgt tgagagcatt
     7741 atctggaaga caggaaatga attaacaaac atgattcgcc ccaacgtaag gaatcggtcg
     7801 cgaatattgg caaaagtctg aaagattaac cactgagcca cggagacaca gagaaaacaa
     7861 attatgtaag agattagatg aattgcaaga tacggacgag atataaaaag ccgaccctgt
     7921 aaaatggatc ggctttttcg tgtaaattcg cggctaaaga cttgaaagag taaattcggg
     7981 atgtctcatg tttatgactt tggatgggtg atgcgtgcat catgttccag gatgtgcctg
     8041 caaatattca aatcttcctc gtgataggcc agaaagaata ttttttggac cttcgtctgc
     8101 ggattttctt ccacatatga aatggccgca tcgatcaatt gcctggagat ttcgtctgca
     8161 tcgagccgcg ttgtacctgt tcccatcaac ggaaagagga tggattggat gttttcagca
     8221 gccatttcct ccgagtcgat gagttccagt gcgttgcgaa cacattcagc gatttctgta
     8281 atgggcatgt atccacgccc aacctgcccg atgactgagg cggcatggaa tatttttttg
     8341 acattgtgtg atttgttcaa ttcacctgaa ccagtgggaa tgacgatccc tgctggcaca
     8401 tcggcagtcc ctgctgcttc gcgcagttca ttcgcgatca aatcgtcaac gacgcgcccg
     8461 gccctgtctc ttttcgcgcc aaggtagcga atggtggccg atatggagcg ttcaaaatgc
     8521 cttgccatct gcatgttggt gttttccgag ttcacccaga cgtcaatttc cttgatgttt
     8581 tgaatgtcac ctgtgatcat tccgatctct tttccagcga ccttggtaat aggatagagg
     8641 tgaatattct tggcgccgat tctctttggt ttgcgttcta cttttagatt atcgagaact
     8701 tcataaattg ggctgtccaa aacctgtccc gcaatcccat tcttgatgaa atctcgaatc
     8761 ttttgttttc cattttccag gttttgcagg tcatctggtt tgtattccag catttgaaac
     8821 ccctgaatgt taaatggggt gggggtgcca gatttacgga tcaaaatggt cacgcttttt
     8881 ttcaacgcat ggcgaactcc cagctcgtaa aacacattag ggttgaggga ggtaatatct
     8941 acaaccgcca catccgcact gaaaatatat tcgaacatct tggaatggat ccagcctgct
     9001 tcagcgattt cgtcacagcg gatgcacttc aggtcaagac cctccacggc gtccttgata
     9061 aagtccctgt aaataaggtc aaaattgatg ggttgtccat caaggtcttt cttttcccca
     9121 aacggcatga taacaaagca ggtctttcca gctaaatttt cttccatgag atttctccgt
     9181 ttatgagtgg gatttcttca acatgcgatt ttccaaccat actatggcca tgatggtctt
     9241 ggcatccaca atttttcctt cgcgaatgtc ttccagagca ttatccaggg tcatttcaag
     9301 cgagatgata tcttcgccat gaccgggtaa tccgccagtt ttattatata aagcgaattc
     9361 cccagatact tctgcgtaat aaagaaaaac catttctgaa ctgcctcctg gggatggata
     9421 aaaactgcaa atggtctcaa acgtagagac caccagacca gcctcttcca acaattccct
     9481 gcgtgccgat tcttcgggct tttctccagg gtcaaccatt cctgctatga tctcggtcag
     9541 ccagccatgt ccatttttgt aggttggata tcgaaactga ttgatcaaaa tgatcttttg
     9601 ggtatcgaga ttgtaaacca ggacagcggc ggaattgcct cgctcaaggc tgaaacggcg
     9661 tactggacga ctcatctccc cgttgaatag ttcgtactgt aaatgcgctt cctctacctg
     9721 gaaaaaatca tcaagggcta gggccttttg ggtgattacc acttttttca taacaccttc
     9781 cttgtttcat accgctaact gttttgaagg gtatatcaga acgattttga attatccagc
     9841 agggatttca cgaataactt caagtatact ttaatccact gccattgatt aatgcgcttg
     9901 aacttttttg taagttgacc gatcttatta tccaaggaca ggcaaatgag tcttccagta
     9961 tacaggcgga agttccagac atttatcagc tccgttaatg ccagtaatga agtggcggaa
    10021 caaattgagc attggctcac agataaagct gggattcctg ttttgcatga tatccaaaac
    10081 tttcagtccg gtgcttcaat ggaagacggc ctggagttat gccaatccat gctcgttttg
    10141 atcaccaaac gcgggatcga gtctggacga gtaaaaaaag aatttgaatt gggggtcaag
    10201 caacaggcca ggtttggaaa tttcttccat gtcattcccg tccgtgttga agaatgcatt
    10261 attcctgatt tcctggagca ttctgccttt gttgacaccg ttgaaagcgg atttgacatg
    10321 tctgctgcga tgaaattgtt gtctggcctt tattccaatg gattggtttc aggagatgga
    10381 aaaactcatg acctttttgt cagttgtccc tggtctcgtg aagaagatgc cttaacaaag
    10441 cttgtcttca aacttgccaa gcggatcggc ttcaggttga ttgggaaccc gattgaaaca
    10501 cacgagaaaa gtgatgtgtc caccagtctc atctcaggat gtgggggagg tattgccgtt
    10561 atcccagacg gtattggtga gaaaaccatg aataagatca tctccgattt ggggattatg
    10621 agtgatttga acttgccttt tctagtggtg atggaaaagg tagtaaattt gccagattta
    10681 atcactcaac atgcccttga aattgtacat cttgataagg atacattatc ccaagatgca
    10741 gatctggaaa aacaaatccg tgcatccgtc attcgcttgt atgaagattg ggtatcacca
    10801 ttagagcagc actatatttt ttatgggact gatctgaagg aagaacataa ggcgagaagc
    10861 cgttttgtgc gtcgcgccat tcagcaaatc acatccatgc cttgtatgat gggagaggat
    10921 atccagcgtg gacagataca gaacgaaatt attaacatga tcatcggcgc gcgaatgatg
    10981 atcgcagata tctcaagaga gaatttaaat acatgcatag aagccggagt tgccatcggc
    11041 tcgaatgtcc cattaaattt gttgtcaggt gatgagcgtc ataaaccacc gtttatgttt
    11101 cgagatcgcc aaatctggca ttatcaaaat gaattggagt tactcggaat ggtccataaa
    11161 cttgttgctc cttatcgaaa attcgtcata tagcaaaact aatgaaattt gtaagcatga
    11221 atagtttatc ggtcagtgtt caggaagtga agatggcgct cgcaatgaga ttgaagtaat
    11281 agagttgcca tttcttgttt ttaaaaagta tagattctgt gttctaaaaa atgatttaaa
    11341 ttggtgcgat tttttaaggt ttaaatgcct tgtagatatt gacaaagttt tagagatgca
    11401 tataattgcc attcgccagt acaaaacaaa agatttcgtc tccccgacaa atcaatgaca
    11461 actgagtaac cgtctagcat cagagacaca aagcaaattg ttttgcagcc tgatgagccg
    11521 atgcgaaaga aaagcatcgg tattttagtc tgtgtaaacc cttgacagac ggttatagta
    11581 gcgagtagaa taccgctcgc tcaaatggca agcaccttaa caactgaaaa gtgataggaa
    11641 acaacatttt tgttgaacca gtaaattccg taacttatac actcgcaaga gtgaaaaatt
    11701 ttttgcggag agtttgatcc tggctcagga tgaacgctgg cggcgtgcct aatacatgca
    11761 agtcgaacga ggtgcttttg tagcaatacg aaagtatcct agtggcgaac gggtgagtaa
    11821 cgcgttggtg acctacccca aagtgtggga taacagtccg aaaggattgc taataccgca
    11881 tgtggttatc agatttagaa actgatatct aaaggagcaa ttcgctttgg gagggacctg
    11941 cgtcccatca gctagttggt gaggtaacgg ctcaccaagg cgacgacggg taggggacct
    12001 gagagggtgg ccccccacaa tggaactgaa acacggtcca tacacctacg ggtggcagca
    12061 gtagggaata ttgctcaatg ggcgaaagcc tgaagcagca acgccgcgtg tgcgatgaag
    12121 gccttcgggt cgtaaagcac ttttctgaga gatgagaaag gacagtatct caggaataag
    12181 tctcggctaa ctacgtgcca gcagccgcgg taaaacgtag gaggcaagcg ttatccggat
    12241 tcactgggcg taaagcgcgt gcaggcggtt cggtaagttg ggcgtgaaat ctcctggctt
    12301 aactaggaga ggtcgttcaa tactaccggg cttgagagtg gtagaggaag atggaattcc
    12361 cggtgtagtg gtgaaatgcg tagatatcgg gaggaacacc agtggcgaaa gcgatcttct
    12421 ggaccatttc tgacgctcag acgcgaaagc tagggtagta aacgggatta gagaccccgg
    12481 taatcctagc cgtaaacgat gtaaacttgg cgtcggtggc ttaaactcca tcggtgccgc
    12541 agctaacgcg ataagtttac cgcctgggga ctacgatcgc aagattaaaa ctcaaaggaa
    12601 ttgacggggg cccgcacaag cagcggagcg tgtggtttaa ttcgatgcta cacgaagaac
    12661 cttacccggg tttgacatgc aagtggtagt gatctgaaaa gtgaacgacc cgcaagggag
    12721 cttgcacagg tgttgcatgg ctgtcgtcag ctcgtgtcgt gagatgttcg gttaagtccg
    12781 ctaacgagcg caaccctcgc cgtatgttac atgtgtcata cgggaccgcc ggtatcaagc
    12841 cggaggaagg tggggatgac gtcaagtccg catggccttt atgtccgggg ctacacacac
    12901 gctacaatgg tcggtacaat gggttgctaa accgcgaggt ggagccaatc ccccaaagcc
    12961 ggcctcagtt cagattgcag gctgcaactc gcctgcatga agccggagtt gctagtaaac
    13021 gcgcgtcagc tatagtgcgt tgaatacgtt ctcgggcctt gtacacaccg cccgtcacgt
    13081 catgggagct ggtaacacct gaagccggta gtctaaccgc aaggaggacg ccgtcgaagg
    13141 tggtgctggt gactgggacg aagtcgtaac aaggtaggtg taccggaagg tgcgcctgga
    13201 tcacctcctt tctagagtaa gtgagcactc cctatgggga agttgctcta aaaatgccca
    13261 cacggtgaat gggcgagctg gttccgaaaa tccgtttcct gtcactgttc agttgttgag
    13321 gaggcccacc cccgggcttg tagctcagtt ggttagagca ctgtgctgat aacgcagggg
    13381 tcacaggttc gaatcctgtc aagcccacta accaggcaat cctggggatg tagctcagcg
    13441 ggagagcagc gcctttgcaa ggcgaaggtc gcgggttcaa atcccgccat ctccactggg
    13501 ttcgtagtat gaacaaacgg agtaaagata agtttgttaa acggtacaaa gacgtaaaaa
    13561 agtaagtaac accgcatttt ggcaagggta ctttatggac ccggcggtgt aacgtcgggt
    13621 cgttttgtgc ctaaaatcga gttgaagggt aatcgatgcc cttgattatg atctagagca
    13681 tcggtcactg gtcaacagtg aagtgaacct taaaaactga atagcaagat gatgtaggcc
    13741 gataaagaaa tttatcgtag taattaataa agcgaaaagt atcaatttct ccgtatcacg
    13801 tggagaagct actaagagct tatggtgaat gccttggcgt caagtgccga tgaaggacgt
    13861 gggacactgc gataagcctc cggggagccg tgtacaggcg ttatatccga ggatgtccga
    13921 atagggaaac caactgttgc gaaccaacag tgtctctggc tgaatatata ggccagttga
    13981 cgggaacctg gggaactgaa acatctaagt acccagagga aaagaaatta ttcccccagt
    14041 agtggcgagc gaacggggag aagcccaaac cgactctgct tgcagagtcg gggttgtagg
    14101 accggcatat ggaagttaca aaacagtcag ttagcggaaa ggtgtgggaa agcctgccaa
    14161 agagggtaat agccccgtat gcgaaaactg attgtcttcc gccgtgttcc tgagtactgc
    14221 cgtgcacgct aatacggcag gaagctaggg agtccactct ctaaggctaa atacacttga
    14281 cgaccgatag tgaactagta ccgtgaggga aaggtgaaaa gtccccctgt taggggagtg
    14341 aaatagaacc tgaaaccata agcttacaag cagttgaagg gctacgacaa gttgagcccc
    14401 ctagaaatag ggacgttcct tgttatgcct gacagcgtgc ctcttggaga atgagcctgc
    14461 gagttaattt cagtggcgag gttaagggag taacacccga agccgtagcg aaagcgagtc
    14521 ttaatagggc gtatagtcgc tggaattagg cccgaaaccg tgtgagctac ccatgggcag
    14581 ggtgaagcga gtctaaacac tcgtggaggc ccgaactcac taacgttgca aagttaggag
    14641 atgacctgtg ggtagaggtg atatgccaat cgaacacgga gatagctggt tctccccgaa
    14701 atagctttag ggctagcgtc atgcgtttag aactggaggt acagcactga atgggctaag
    14761 gggcttacag cttactgaac ccaatcaaac tcggaatgcc agttctccat agcatggcag
    14821 tcggactccg ggagatgagt ttcggggtcg aaagggaaac agcccagatc atcggctaag
    14881 gtcctaaaat ctatgctaag tgggaaacga ggtgaggtta cttaaacaac caggaggttg
    14941 gcttagaagc agccaccctt taaaaagtgc gtaacagctt actggtctag tgacttcgcg
    15001 cggaaaactt aacggggcta agcatagtac cgaagccatg agtctcaacg caagttgggg
    15061 cagtagggga gcgttctgtc agcggtgaag gtcgatcgta agggcgactg gagcggacag
    15121 aagtgagaat gcaggcatga gtagcgtaca aacacgtgag aaccgtgttc gccgtaagtc
    15181 taaggtttcc gacgccaggt cattccgcgt cgggttagtc gagtccttag ccgaggccga
    15241 gaggcgtagg tgatggacat ccggtcaaca ttccggaacc gctagagagt gcgatgggag
    15301 gacgcaacga ggtaggccag cccattattg gatttgggtt cgatccatct aggtgttgag
    15361 actggtaggt aaatccgcca gttgagctga ggtgggagcg ggccgtaagg cgaagtggct
    15421 gagccttgtt gccaagaaaa gtctctaagc ataactttct agtgctcgta ccgcaaaccg
    15481 acactggtag acgggtagag tataccaagg cgaacgggag aaccctcgtt aaggaattag
    15541 gcaacatgac cccgtaactt cgggagaagg ggtaccctct agggtgttaa agccctggag
    15601 ggtcgcagtg aaatggctct ggtgactggt taacaaaacc acaggtctct gctaagccgt
    15661 aaggcgatgt ataggggctg acgcgtgccc agtgccggaa ggttaaggag agaggttagc
    15721 gcaagcgaag ctttgaaccg aagccccggt gaacggcggc cgtaactata acggtcctaa
    15781 ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaccgcgtaa caattagagc
    15841 actgtctcga cgagggaccc ggtgaaattg aaatggctgt gaagatgcgg cctacccgca
    15901 gcaggacaaa aagaccccgt ggagctttac tgcaacttgg cattgtattt ggacatgatc
    15961 tgtgtaggat aggtgggagg ctttgaagct ggcgcgctag cgtcggtgga gccgtcggtg
    16021 aaataccacc ctgattatgt ttagatccta accccattcc gtaatccgga tgggggaccg
    16081 tgccaggtgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga ggcgcccaaa
    16141 ggttccctca ggtggaatgg aaacccacca tagagtgtaa aggcataagg gagcttgact
    16201 gtaaggccaa cgcgccgaac agagacgaaa gtcgggctta gtgatcccac ggtaccgagt
    16261 ggaagggctg tggcttaccg gataaaagct accccgggga taacaggctg gtgagatcca
    16321 agagttcaca tcgacgatct cgctcggcac ctcgatgtcg gctcatcgca tcctggggct
    16381 ggacaaggtc ccaagggtcg ggctgttcgc ccgttaaagc ggtacgcgag ctgggttcag
    16441 accgtcgtga gacaggtcgg tctctatccg ctgtgggcgt aagggatttg aagggagctg
    16501 ctcctagtac gagaggaccg gagtggacag acctctagtg tgccagttgt tccgccagga
    16561 gcatcgctgg gtagccatgt ctggcagaga taaccgctga aagcatctaa gtgggaaact
    16621 cgccctaaga ttagatccct gtattcccgt aagggagtaa gaccgcaggt agactacctg
    16681 cttaataggc agcatgtgta agcacagtaa tgtgttaagc taagctgtac taatggtcga
    16741 gggcttcctt ctcgtgatac ggagaagttg gtacttttca gaaaactaca tcatatattt
    16801 gctatttggt gacaatcttg tgaaattgtc ccttggtgat ttgagcgacg tgggtacacc
    16861 cggtcccatt ccgaacccgg aagttaagca tgtcagcgcc gatggtactt ggagggcgac
    16921 ctcctgggag agtaggtcat tgccaagggg ctttt
//
LOCUS       DAOQLC010000097        16345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1281_length_16345_cov_13.482198, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000097 DAOQLC010000000
VERSION     DAOQLC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_16345_cov_13.482198"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(508..735)
                     /locus_tag="PL000_15460"
     CDS             complement(508..735)
                     /locus_tag="PL000_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90331.1"
                     /translation="MPFLAAWNMIPDPLKKFVVGLLIVLLIVLLGVSFRAVSCLGHKA
                     TEGGTSEKINHSNVPDDSVFDSIIHSLPINR"
     gene            complement(738..1088)
                     /locus_tag="PL000_15465"
     CDS             complement(738..1088)
                     /locus_tag="PL000_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90332.1"
                     /translation="MNKRIIMYFGIAIATVGVGVMIFASIEQATQSMELTTSGIKSTG
                     AYIGAVLLILVGAVFIIYGTSTIKADFAHVDIESEAEAKPPDPKPIPPPTEVKLEGPV
                     TTITGAPVQKKEGG"
     gene            complement(1099..1755)
                     /locus_tag="PL000_15470"
     CDS             complement(1099..1755)
                     /locus_tag="PL000_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90333.1"
                     /translation="MKKLIIKWLIDLHEKLREKEWARNANKTLEGLYLRYFGWFRGNA
                     PLRIIGDSIAASLLMINDRFGACENQGIPGEKTEGMLFRFALAIKGKPKKLACSIGGN
                     DLRDNVPVSDVVGRIFQMVERARSAGAEFGWIEPPALGPAFADLNLKVPLLISAVEIE
                     NARRQLKGLPTLTIIRVRKEAQGSDGYIKPEYFGDGIHPNYMCLSIVYFSAINSFFQA
                     "
     gene            complement(1752..2102)
                     /locus_tag="PL000_15475"
     CDS             complement(1752..2102)
                     /locus_tag="PL000_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90334.1"
                     /translation="MGTEPPDYWNGEIGKAKLTQAARAHIKSIWLVYGDDWINKIVPS
                     QKARIDATGRFVSAGWLQHRDVPYWTGSAWAQPPEDNIKAGQHADIHKDFPMETFFMV
                     LAAEINRSVKEGVK"
     gene            complement(2543..2884)
                     /locus_tag="PL000_15480"
     CDS             complement(2543..2884)
                     /locus_tag="PL000_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90335.1"
                     /translation="MWNSLRSMVKISEDSRVEVSWKIAATIMCAMIGGSVALGAYMAR
                     LDDTVESNQMLKEKVDKIDRNLERVCYALNEALETRKKTTLPCDRMQDPKRYGALTTV
                     YENAPVPDSVF"
     gene            complement(2956..5370)
                     /locus_tag="PL000_15485"
     CDS             complement(2956..5370)
                     /locus_tag="PL000_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90336.1"
                     /translation="MTAISNTRYDHLRRDFAWRSMDFEVRQCGVIDELTEIDGTEHYG
                     VYLQDHMHGVICAPMRANSSALLISDITSLGGNQYRFNFTGALDGSVVVGDFLHVKGA
                     TNEDNNGRYEIVSKGTAYAVVINANGAAQASGGTVVCAISYSPMIETQSAPSAGQYSP
                     DYTSGSGIVRLNASRSTEYFVLQYEGGGSIDRIGVGGKGSGGGGGGTGINLLSNGDGE
                     TDTTGWATYADAAGVEPVDGTGGSPNISWTLTSTSPLRGNGSFLLTKDAANRQGQGVS
                     YDVTIDNADKGQIIEFRGEGRLVSGVYDSDIRVFVYDVTNANVIEFSPRDLESAGAAV
                     PFTFKKSLQVPINATVLRICIHIASTSTSAFSMVFDTLEFGPVVRAYGSPITDMKEYG
                     PLTIGAVTTPPSKGTVVLERAMWCRMGSYAFISYEYIQSSSGSGGSGVYKYPMPPGLK
                     IDHSRASFRGDNGITSRGILGHGWLTGDGSSGSGATVRAFDADNLSLLTEEGAFADNG
                     DTQYSFSNSSLRIHFTAMVPIVGWSANAEVNSDYDTRLMTCRASGTPASASAGNPIIF
                     PTVDFDSHGGYDAATGEYTAKFAGVIDITGWIDCSTANLRIAVFINGSQDTVIGGTGS
                     TLTAGSIAGSIKVKADDVITVRPLASGTGAFVAGGFISFSRKTGASQVAASETISASA
                     YVSANYAVNANEAIDFDTVLFDSHGMITPGSGNTWIATAPASGQYLISGNGKCAIATP
                     GIQLYKNGSALSYVTVLQGNVPNSFAVLVDLKQGDTISLNSLSTTTIDGGSAPYVTQV
                     SIIRCR"
     gene            complement(5367..6986)
                     /locus_tag="PL000_15490"
     CDS             complement(5367..6986)
                     /locus_tag="PL000_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90337.1"
                     /translation="MSLFGSTNTDARFDEIDQVFVPNHITAEVKTIRPLAGQPGRYGI
                     RLNEFPLRWDDPVSFSVPNPSVIISGYTMVSTDPAASEFRPGFYFDEGSPPVVFRSNI
                     VYFHASQNGVQVSVEYWGGGTHLAASLIQTKTIDKNKLPNLTWSGTPVNQRVIVANDI
                     TLTDDTVVKARMIVCFGTFSIATGKKITFKAQATKTAVRTNDRFEGASRAGSLGGQNA
                     YYVSAGPVIHNGEDGVAGLAGHDGLRGIFTTERRSGKGGDGGDGGPVDLYDGGSVLDT
                     YPGGSGESGGSDSGGLSASGGSGAGGGSCGSTSFTAAHTAIGGAGGDSGQGGPRVIIL
                     CLGDFINFGTILCDGDDGSAGADGAIATDTSPSAAAFGGTGGGGGAGGGAGGSLCVWV
                     SGDYNGGTMRAKGGDGGDGGDGSAHSVYSGQATELTDPWPTYRLFGGNGGDGGDGGDG
                     GHIEVYARTSSFGTITAPKGLKGLKGVGGAGTAGPRVGADGSDGADGVDGDVLTCDTD
                     SNPVSLLGAIASSEISGILFSEAEGVYGLVA"
     gene            complement(6988..8922)
                     /locus_tag="PL000_15495"
     CDS             complement(6988..8922)
                     /locus_tag="PL000_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90338.1"
                     /translation="MSFRSVFSAGFGYAPLGLLPGGGVSRFSVFSFPDLGSEPRIHVF
                     APDGIDIGYLPLDIQDSQVSKFRFRYGVTGSLDFSIELNAEPEFPQVRFAEMLFYIGN
                     RKAYRGYISKYPGPEKKISDPWRIAGLSMSARFDKKKIALKSGVNLYRIDSISVSGSY
                     VTITLDQDLGTDEVLNCVALVQDATDSDNNGRYQITAFGVNQITFFNPAGHNQALPVG
                     VVRILPLAWSSPITLVSDLIKQVIENYTDDIPIITDTSLIEDSTGIVTGDTVDLDGMK
                     MTDFFAHMRTLAQDYRLYIDEDKRVVFQKKSTSLVAIIIIGYEINETQEVLDDSEVIN
                     RWYVNQKKDRTVTAPQGFKAGAVSSDATSIANYGLLESEQDVPVYYGTGLCQAMADAL
                     VQSTKDPKNTITAKNVPFDYYELGLYKIITHPRMRDTVLHECETLTGWTYNAERMKAS
                     IITDNVIYGNAAIKIAYDWYAEGEVFYLSPNLPIMKPERLRFWYRGNRIGQKITFMYG
                     ENSYTENEVSFSCYTTRWRPFDVDLTAFTGSKLGQFGFRLDDSGEVLRQATFGSGLMG
                     TAFAWGNRYSEVSNFFIDEITLYSLSSEHKTIELTEVEVVAEDTQVCNLTFGPRLEGM
                     PEYLAKQFRNLETARIALRS"
     gene            complement(8931..9767)
                     /locus_tag="PL000_15500"
     CDS             complement(8931..9767)
                     /locus_tag="PL000_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90339.1"
                     /translation="MTRSYEYQVRNVHGDLLDLSSQRYRTSVPKSSRNYALADRLEQG
                     GSVLLGDGKIKGRKINLKFDYAGGGASRAAQHNDVHQLMNTLASFFRIQDAPFYLENL
                     VRNTRTRVYGDFSPSHMDGNEFLIQQNSTIELDVLDAAWEDLTETSSSVVTMASGDTL
                     DLSPALPNYCEDIFPVIEVTARADSPTTFSIETGRDPGTGFVVFRTILLSEATFTLGR
                     VITIDSRNGKILIDGRSAPEIISFGFPVKFDRRNQRLRYYSSTGTGSVDVQIRHRVRT
                     LY"
     gene            complement(9770..12589)
                     /locus_tag="PL000_15505"
     CDS             complement(9770..12589)
                     /locus_tag="PL000_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90340.1"
                     /translation="MKELVTRIRLESKDFISDMGSAKNSALALSAAVAGVATGMAALA
                     IHAAETIDKNTVLARSAGEATDRFSAMSYAMKLGGVDAESLAAGMAKMARPEQQKNLV
                     AMGIAVTDATGKLRSQTDVMLDLAGVIEKTNDPLRRLEIAQSALGKSGGQFIEALKDG
                     PAALRELMTEADALGQVFSKTDAIAADQFNDNLDKLKMGVSGVSTEIGKSIVQWVNHT
                     KILDGAIGIVKNVISMWRNMDQGTKEFVYTFGTALVATTALVGVLYGVIQVAPLVSAA
                     IKAAFITNPIGLFVTAIALVAAGVIYMKSRWEEFQAFFAPLTAMIRNFQSQVSSGIAS
                     VIGIFSRFSSTLSPIKGIFDSIAGAFKNVDLVATGLKAAMAPMLITFYTLATVINVLV
                     TAFDALIQVLRTPVGEGLMKFMAGSMTGNMVMMAEGVQKVSQGWGEASEIVKKASGDM
                     KKSLSSLSDNVSTTLKNIVTKSAPAAQAMDNLFNSADRKLNIGPMETFETFGNEFYKI
                     YENAIKAKEGFAAAGLAGMDFKSRMQAIALGIEGVKGVFSSLSGMFTQYLQSLQKLSQ
                     TRLTNFTQNLDFMAGGAAKMLELQLAADNAFYDAQIKGLQKQKDALLDEERRYQDDLK
                     ALKDSYAQQRKAELDEEVKAEIRRLDDQYQAQVAYLEQSGVTGTELEARKADLLAQIE
                     AQKNQAISESQKRLQNDITNRNSQLDEEAKVHATTAKQREDDLALHIQMIEDQKAASA
                     QATADKKAMIERQTKLMEWNAGRATFEIGKRAQMAAAVASVAMGVMNAIQAGVLLAAT
                     IPFAGWIMGPILAGTLSALVIAAGARTVSSISAQQYPPPPLFGDGGIAMGGAGGMGII
                     GDNGPEIAMPMNNPGKDFGALKGELASQLADAASGAMGGTRVADKIEVNLTNVFQPRD
                     DYEMIKSKISVDLRDMVRGVIESVR"
     gene            complement(12586..12891)
                     /locus_tag="PL000_15510"
     CDS             complement(12586..12891)
                     /locus_tag="PL000_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90341.1"
                     /translation="MFDTFWPDEVEEWSEVAEALRIEEFLERMSAAQYGQMIPDEEGK
                     VAESKKKMVESLDDERRILMGLKTRVQMLADKTRSWIEAKKKAKMEKEYREAKEERK"
     gene            complement(12945..13277)
                     /locus_tag="PL000_15515"
     CDS             complement(12945..13277)
                     /locus_tag="PL000_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90342.1"
                     /translation="MKLKLQKPGSDRVLEVELPDGKVKKLIIRRLSVREADERDKEIN
                     RIQAEVKDGKLSSTDGAIKVLGKYVKPETDLKWVWDLEDLDYVTQIIESLTLVVAGKA
                     EASEEEKK"
     gene            complement(13274..13864)
                     /locus_tag="PL000_15520"
     CDS             complement(13274..13864)
                     /locus_tag="PL000_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90343.1"
                     /translation="MPAITNPPGILADGKYALGPGRLFLDTETSGGINKDAGPTNEIK
                     WSIEMNWIGLRSADYGDRDDESVASSSQTMVECGLAKPDWDTLLLIHPCLEGEFDTDG
                     NLIAVHFVDARGYRTEDHRMQITFKEYDPHTNIVAVDPWRIVDFYQAAAMIEKADLTW
                     DAKTQRYFPVKFQAKPDPDHLSPLGRPVAWKIRGDS"
     gene            complement(13922..15085)
                     /locus_tag="PL000_15525"
     CDS             complement(13922..15085)
                     /locus_tag="PL000_15525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90344.1"
                     /translation="MSDNLLRRLQAYHSGDLMIIPKKGIAYQATKDRIQYTDEYIESC
                     RKYKENPDRMSAILAGRNQIVREYVKEEVEVLDVGVGAMDFLESRDRTYGYDIVPETV
                     SKLKKAGKYSDDFSRFRAFTLWDVLEHMEDPSRFLRRIPDRSFVFVAIPTFHEPSFTG
                     IESWPHYKPGEHLYYFTELGLIEFFRMNGFQLLCTNNSEIEAGRRDITLAAFIKDAPS
                     YNDMIGMYEEMHQKRYGVSAADHLEQIAGLVRANKPNSIIDYGCGQAGFGNYFWRDGE
                     RRIYLYDPAIPQYKDFPTGSAFDLALCCDVMEHIEMRHVDRVLEEIRSVSRNAIFTIA
                     TKPARAKLPDGRNAHVTLLSPEEWREWIEDVFGFAEMEPTVHPKTVLVRTFKP"
     gene            complement(15182..15895)
                     /locus_tag="PL000_15530"
     CDS             complement(15182..15895)
                     /locus_tag="PL000_15530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008373 - sialyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 29 protein"
                     /protein_id="HNC90345.1"
                     /translation="MRTKTNFISLDQMRARFKGKSIAVIGSGPTALLNSGEHIDSHDL
                     IIRIQNYKMRGFESQVGSRTDVHYSFYGMSMRKTANELKEDGVSLCMCKLPDGALPFS
                     SPWHRQNNKQHGVNYRYVYTARAAFWFCDTYVPSLSRFMELFALVQNEDDKYPGHQPT
                     TGFACLLELMQCETKSVYVTGFDFFTSNKHNLNESWAPKNPDDPIGHRPLKELKLLKK
                     IFPANWKADRSLEELMKSC"
     gene            complement(15879..>16345)
                     /locus_tag="PL000_15535"
     CDS             complement(15879..>16345)
                     /locus_tag="PL000_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90346.1"
                     /translation="VYQNLRAPMGRSDGFGFETIPSIPSVRAAIKALPDHKLIKIGQG
                     DSDDIRADEDLTGKTSISESISYFCAADIIIGQCSWIIPLAECRKIPLIVLFGHCIRS
                     AEHQFIRSISPEKLLLRHKRNGNLEIGLWDDDSMIEQKVKTWESGLVHAHQN"
BASE COUNT         4083 a         4531 c         3614 g         4117 t
ORIGIN      
        1 atagtagacc ataccttccg gattcgttca actacttttt tacagaacac atggtttttt
       61 taatgttatc tgcttttgag ggccgcttgt gtgccccgtg taagcaagat ttcagagggg
      121 gtagccatag tagcacggag ggcaaaaaat acccccggtt tttacaccgg gggccaggct
      181 cacggagttt cagaaatggt tcaaggtttc ggacaggatg gcttctgcaa atcgcaagaa
      241 tacgttatgc caaacagagt tcgtaacgga ttagtaaatg cgcgatccgc agcctgccat
      301 ccgcagtttc catcgtatgc gagccacatc atcgcgcatt cagatgcgcg aatgcatttt
      361 gcttctgcgc gcgcaaactc cacccagtcc tgcgtcactt ccattcgatt gtagtatttt
      421 ttctcaacta caatccaatc ctcgggcttc atgtaacccg gtgactcagg aagcgcacag
      481 gcaacattcc gatccgcgat ttttctatca gcggttgatc ggcaagctgt gaatgataga
      541 gtcaaaaaca gaatcgtcag gcacattgct gtgattgatt ttctcactgg ttcctccttc
      601 ggttgctttg tgtcctaagc atgatactgc tcggaaggac acaccgagca gaacaatcaa
      661 aagaacgata agaagcccca ctacaaactt cttcaacggg tccgggatca tattccatgc
      721 tgcgagaaac ggcatcgcta tcctccttct tttttttgaa ccggcgcgcc agtgattgtg
      781 gtgaccggtc cttcaagttt aacttcagtc ggtggcggta tcggcttcgg gtccggaggt
      841 ttcgcttctg cttcggactc aatgtcgacg tgcgcaaagt ctgccttgat cgttgaagtt
      901 ccgtaaataa taaatacggc accaaccagg atcagaagca ctgcaccgat atacgcacca
      961 gtggatttga ttccagaagt tgtcaactcc atcgactgag tcgcctgctc gatcgatgca
     1021 aagatcatga cgccaacacc gacagtggca atcgcaatcc cgaaatacat gatgatccgc
     1081 ttgttcatgg tttcctcctc aagcctgaaa gaatgaattg atcgcagaaa agtaaactat
     1141 gctcaagcac atatagttcg gatgaattcc atcgccgaag tattccggct taatatatcc
     1201 atcggagcct tgcgcttcct ttcgcacgcg gatgatcgtg agagtaggaa gtcccttcag
     1261 ctgacgtctt gcgttttcaa tttcaaccgc actgatcaaa aggggaacct tcaaattcag
     1321 atccgcgaat gcagggccaa gagccggtgg ctcgatccag ccaaactcag cgccggcact
     1381 gcgcgcacgt tcgaccattt gaaagatgcg accgacgaca tcggagacag ggacattgtc
     1441 ccttaaatca tttcctccga tactgcacgc tagctttttc ggttttcctt tgatcgcaag
     1501 agcgaagcga aacaacatgc cctcggtctt ctcgcctggg attccctgat tctcgcatgc
     1561 gccaaaacga tcattaatca taagcaaaga tgcagcgatc gaatctccga tgattcgcag
     1621 cggcgcattt ccacggaacc agccaaaata cctaagatac aatccctcta gagttttgtt
     1681 tgcgttgcgc gcccattctt tctcgcgcaa cttctcatgc agatcaatca gccatttgat
     1741 gatgagcttt ttcattttac gccttccttc actgatcgat tgatttctgc cgcgagtacc
     1801 atgaaaaacg tttccatcgg gaaatccttg tgaatgtccg catgctggcc cgctttaata
     1861 ttatcttctg gtggttgcgc ccatgcactg ccggtccaat aaggaacgtc gcgatgctga
     1921 agccagccag cggaaacaaa tctaccggtc gcatcgattc gcgctttctg tgacggtacg
     1981 attttgttaa tccaatcatc accgtagacc agccagatcg atttgatgtg agcacgtgcg
     2041 gcctgcgtga gcttcgcttt tccgatttca ccgttccagt aatcaggagg ctcggtgccc
     2101 attccgccaa tctcgatctc ccatgaatcc tcggcagtga tccaattacc gcatccccag
     2161 catttaccgt ctgcaggcag acaaaggaca tacgaatcgc tatcacaagt ggcgtgaacg
     2221 gaagcatagc gccggccata cttggccgcg tccaggcgat tctctggcgg gtccgctaaa
     2281 tactttgcga caatctctgc agtggggtca tcctttccgg gcccgacaat tgcctcatac
     2341 accgggacat gtagcacggc caatcttggc cctgtggacc gtccagtgcc gcacgtttga
     2401 aagttcccaa gtcggtgctt atacgcatca ggggcgcaca aaacgtgcgg atgcgtaaaa
     2461 ttgtaatagt cgtttgctga gtccaaatga tgcctccagt gaatctggaa acatcgcatc
     2521 ccatgtcatc gcacaaatca agtcagaaaa ctgaatctgg aaccggtgca ttctcataca
     2581 ccgtagtaag cgctccgtat ctttttggat cctgcatccg atcgcacggc aaggtggtct
     2641 ttttccgcgt ctcaagcgct tcattcaagg catagcatac tcgctcaagg ttccggtcga
     2701 tcttatccac cttctccttc agcatttgat ttgattcaac cgtgtcgtcc aggcgcgcca
     2761 tgtatgcacc cagagccacg gaaccaccta tcatcgcgca catgatggtg gccgcaatct
     2821 tccaggagac ttcaactcgg ctgtcctcgc tgatcttcac catagatcgt aaagaattcc
     2881 acatcgcggg cctcccgtct attgtatttt ctcagtttac ctgcgcaaaa tgattctaga
     2941 cacaaatgcc taaaatcatc tgcaccgaat gatgctcacc tgagttacgt aaggagcgga
     3001 gcccccatca atcgtcgttg ttgaaagtga attcaacgag atcgtgtctc cttgcttcaa
     3061 atcaaccagg actgcaaagc tatttggaac gtttccttgc agtacggtca catacgaaag
     3121 cgccgatccg ttcttataca actgaattcc gggagtcgca atcgcgcatt tcccattacc
     3181 agaaatcaga tactgcccgg acgcaggcgc tgtggcgatc caagtgtttc ctgatccggg
     3241 agtgatcatc ccatgcgaat caaacaacac ggtatcaaaa tcaatcgcct cattcgcatt
     3301 cacagcgtag ttcgcagaga cataagcact agcagaaatg gtttctgatg ctgcgacttg
     3361 cgaagcgcca gtctttctgc tgaaggaaat aaatcctcct gcaacgaaag cacctgtgcc
     3421 ggatgcaagc ggacgcacag ttatgacatc gtctgccttc actttgatcg agcctgcaat
     3481 cgagccagcg gtcaatgtgc ttccggtgcc gccaattacg gtatcctgtg aaccattgat
     3541 gaaaacagca atacgaagat tcgcagtaga gcaatcgatc catccagtga tgtcgatcac
     3601 tcctgcaaac ttcgcagtgt actctccggt ggccgcatca tatccaccgt gcgaatcaaa
     3661 atctacggtc ggaaaaatga tcgggtttcc agctgatgcg gacgcaggcg ttccggatgc
     3721 gcggcaagtc atgagcctcg tatcgtaatc ggaattcacc tctgcatttg cagaccagcc
     3781 gacaatcgga accatcgcgg tgaaatgaat acgcagactt gaatttgaga aactatactg
     3841 agtatctccg ttatctgcga aagctccttc ctcagtgaga agcgaaagat tatccgcgtc
     3901 aaatgcgcgc accgttgcgc cgctaccgct actgccatca ccggtgagcc agccatgtcc
     3961 aagaatgccg cgcgaagtga taccattatc accgcgaaat gaagcacgac tgtgatcgat
     4021 tttcaggcca ggcggcattg ggtacttgta aacacctgag ccaccgctac ctgatgatga
     4081 ttgaatatac tcgtaactga tgaacgcata tgatcccatg cgacaccaca tcgcgcgttc
     4141 aagtacaacc gttccctttg aaggtggagt cgtgactgca ccgatggtta agggaccata
     4201 ctccttcatg tccgtaatcg gtgatccgta cgcacgaaca accggtccga actcaagcgt
     4261 atcgaaaacc atcgagaatg ctgatgtaga agtcgacgcg atgtgaatac agatccgcag
     4321 aacagtagcg ttgattggta cctgaagact tttcttaaaa gtgaaaggga cagccgctcc
     4381 tgctgattcc aaatcgcgcg ggctgaattc tatcacattc gcattcgtta catcgtaaac
     4441 gaaaacgcga atatcagaat cataaacacc ggagacgaga cgtccctctc cacgaaactc
     4501 gatgatctga cctttgtccg cattatcaat cgttacatca tagctcactc cctggccctg
     4561 acgattcgcc gcgtcctttg tgagcaaaaa cgaaccattg ccgcgtaacg gcgatgtgga
     4621 ggtcaacgtc cagctgatgt ttgggcttcc accagttcca tctaccggtt caactccagc
     4681 ggcatccgcg taagtcgccc agccggttgt atcggtttca ccgtctccat tgctcagtaa
     4741 attgattccc gttccgcctc cgcctcctcc ggaacctttt cctccgactc caattctatc
     4801 gatgcttcct ccaccttcat actgcagcac aaagtattct gtgctgcgcg aagcattcag
     4861 gcgaacgata ccagaaccag aagtgtaatc cggagaatac tgcccagccg atggagcaga
     4921 ctgagtttcg atcattggag aatatgaaat agcgcaaact acggttccac cgcttgcctg
     4981 agcggcaccg tttgcattaa ttacgacagc gtatgcggtt ccttttgaaa caatttcata
     5041 tctgccattg ttgtcttcgt tggtcgctcc cttcacatga aggaaatctc cgactacaac
     5101 agaaccatct agcgcacccg tgaaattaaa gcgatactga ttgcctccaa gacttgtgat
     5161 gtctgagatt aatagagcgc tggaattcgc gcgcatcgga gcgcaaatga caccgtgcat
     5221 atggtcctgt agatacacac cgtagtgctc cgttccatcg atttcggtta gctcatcaat
     5281 taccccacat tgccgaactt caaaatccat cgagcgccat gcgaagtcgc ggcgaagatg
     5341 atcgtatctt gtgtttgaaa ttgccgtcat gctactaacc cgtaaactcc ctctgcttct
     5401 gaaaacagaa ttcctgaaat ttccgatgat gcaatcgcac caagaagcga aactgggtta
     5461 gaatcagtat cgcatgtgag aacatctccg tccacgccat ccgctccatc agacccatct
     5521 gctccaacgc gcgggcctgc ggtgccagct ccacctactc ccttcaatcc ttttaatccc
     5581 tttggtgccg tgatcgttcc aaatgatgat gtgcgcgcgt agacttcaat gtgaccgcca
     5641 tcaccgccat caccgccatc accgccattt ccaccaaaca atctgtatgt gggccaggga
     5701 tcggtaagct cggtagcttg accggaatac actgaatgcg cagaaccgtc tccgccatca
     5761 ccgccatcac cacccttcgc tcgcatcgtt ccgccattgt aatctcccga cacccatacg
     5821 cacaaagaac cgccagcgcc tccccctgcg ccacctccgc ctccggtgcc accgaatgcg
     5881 gcggctgaag gcgatgtatc agtagcgatt gcaccatctg cgcctgcaga tccatcatca
     5941 ccatcgcaaa gaatcgttcc gaaatttata aaatcgccaa gacacaaaat gatgactcgc
     6001 gggcctccct gaccactatc accacctgca ccaccgatgg cagtgtgggc tgctgtaaac
     6061 gacgtagagc cacaagaacc tcccccggca ccagaaccac cagaagcgct caaaccgcca
     6121 gaatctgaac cacctgattc tcccgatccg ccaggataag tatcaaggac gctcccgcca
     6181 tcatatagat ctaccggtcc gccatcaccg ccatcgccac cctttcctga tcttctttcg
     6241 gttgtaaaaa ttccgcgtag accatcatga ccagcaagcc ctgccacacc atcctcaccg
     6301 ttatgaataa ccggaccagc tgaaacataa tatgcatttt ggccgcctaa cgaaccagcc
     6361 cgcgatgctc cctcaaaacg atcgttcgtt ctgactgcgg tttttgtggc ctgcgctttg
     6421 aacgtgattt ttttaccggt cgcgatggaa aacgtgccga agcaaacaat catgcgagct
     6481 ttcacgaccg tgtcgtccgt gagagtaata tcattcgcaa cgatcactcg ctgatttacg
     6541 ggagtgccgc tccatgtgag attcggcagc ttgtttttat caattgtctt tgtttgaatc
     6601 agactcgcgg caagatgagt cccacctccc cagtattcaa cacttacctg caccccgttt
     6661 tgtgatgcgt gaaaataaac gatattggag cgaaatacga ctggcgggct tccctcatcg
     6721 aaatagaatc cgggcctgaa ttctgatgca gccggatcgg tcgaaaccat tgtgtatccg
     6781 ctaatgatta cggaaggatt cggaacagaa aaagaaactg gatcatccca gcgcagagga
     6841 aactcattca ggcgaattcc atatctgcca ggctgacccg caagtggcct gatcgttttt
     6901 acttctgcag taatgtgatt cggcacaaag acctgatcga tttcatcaaa tcttgcgtcc
     6961 gtgttcgtgc ttccgaaaag actcatctca actcctcaac gcaatgcgcg ccgtttcaag
     7021 attcctaaac tgctttgcca gatactctgg cattccctca agccttggac caaaagtcaa
     7081 attgcatacc tgcgtgtctt ctgctaccac ttcgacctca gtcagctcaa tagttttatg
     7141 ctccgatgaa agagagtaca aagtgatttc atcaataaag aaattgctga cttctgaata
     7201 gcgatttccc caagcgaaag cagtgcccat cagcccggag ccgaatgtag cctggcgcag
     7261 aacttctccg gaatcgtcca ggcgaaaacc aaactgcccc agcttactgc cagtaaatgc
     7321 ggtcagatcc acatcgaacg gcctccaccg agtcgtgtag catgagaatg aaacttcgtt
     7381 ctcggtataa ctattctcgc catacatgaa agtgattttc tggccaatcc gattccccct
     7441 gtaccagaat ctcaagcgct ccggtttcat gatcggaagg ttcggagaaa gatagaaaac
     7501 ctctccctcc gcataccaat cgtatgcgat tttaattgcg gcattaccgt agatcacgtt
     7561 gtcagtgatg atcgaggcct tcatgcgctc ggcattgtaa gtccagccag tgagcgtttc
     7621 gcattcatgc agaaccgtgt cgcgcatacg gggatgagtg atgattttgt acagacctaa
     7681 ctcgtagtaa tcaaagggaa cgttctttgc cgtgattgtg ttttttggat ccttcgtgct
     7741 ttgaacaaga gcatccgcca tcgcctggca taacccagtg ccgtagtaga caggaacgtc
     7801 ctgctcgctt tccagaagtc cgtagttcgc aattgaagta gcatcgctgg agacagcgcc
     7861 ggccttgaat ccctgcggag ccgtaacagt acgatccttc ttctggttca cataccagcg
     7921 gttgatgact tccgaatcat ccagaacttc ctgcgtctca ttgatctcat atccaatgat
     7981 gatgattgca acgagcgatg tgcttttttt ctggaaaacc acacgcttgt cctcgtcgat
     8041 atacagccgg taatcttgcg cgagcgttcg catgtgcgcg aagaaatccg tcatcttcat
     8101 gccatctaga tcaaccgtat ccccggtgac gatcccggtg gaatcctcga tcaaagatgt
     8161 gtcagtgatg atcggaatat cgtccgtgta attttcgatc acctgcttaa tcaaatcact
     8221 cacaagcgtg atcgggctgg accacgcaag aggtagtatg cgaacaacgc caaccggcag
     8281 cgcctggtta tgtcccgcag gattgaagaa cgtgatctga ttcacgccaa acgcagtgat
     8341 ctgataccgc ccattgttat cggagtctgt cgcatcctgc accagcgcta cgcagttcaa
     8401 aacctcatcg gtcccaagat cctgatccag agtgatcgtc acataggaac cggaaacaga
     8461 aatggaatca atccgataga gattcactcc ggacttgagt gcgattttct ttttatcaaa
     8521 acgagcggac attgaaagac ctgcgatcct ccacggatca gaaatctttt tctcagggcc
     8581 cggatacttc gaaatgtatc cccggtatgc tttgcgattc ccgatataaa aaagcatctc
     8641 agcaaaacga acctgcggaa attccggttc agcgttcagc tcaatgctaa aatcaagcga
     8701 gccagtcacg ccgtaacgaa acctgaattt tgagacttgc gaatcctgaa tatctaaagg
     8761 caggtatccg atgtcgattc catctggcgc aaaaacatga atacgtggct cagatcccaa
     8821 atctggaaaa gaaaaaacag agaatcgaga tactcctccg ccaggcaaaa gcccaagtgg
     8881 agcgtatcca aaccccgcag aaaatacaga cctgaagctc atcaatcacc tcaatacaga
     8941 gtgcgcacgc ggtgacgaat ttgcacatca accgatccag tgcctgtgct tgaatagtac
     9001 cgtagtctct ggttgcgtct atcgaatttt accgggaatc cgaatgaaat gatctctgga
     9061 gcggagcgtc catcaatcaa aatctttcca ttacgagaat caatagtgat tacacggcca
     9121 agcgtaaagg tagcttctga taaaagaatc gttcgaaaca ccacaaaccc ggttccagga
     9181 tctcgcccgg tttcaatcga aaacgtagta ggggaatccg cacgcgcagt gacttctatc
     9241 acggggaaaa tatcttcgca gtaatttgga agcgcaggac tcaaatccag cgtgtcaccg
     9301 ctagccatag tcacaacaga tgaacttgtc tccgtcaaat cttcccatgc agcgtccaaa
     9361 acatcaagct caatggttga attctgctga atcaaaaact cgtttccgtc catgtgcgaa
     9421 ggagagaagt ctccgtagac gcgagtgcga gtattcctga caaggttctc cagatagaaa
     9481 ggtgcgtcct gaattctgaa aaacgatgca agcgtattca tgagctggtg cacatcgtta
     9541 tgctgtgccg cgcgacttgc tccaccgcca gcgtaatcaa acttcagatt gatctttctg
     9601 cctttgatct tcccatcacc cagcaaaaca gatccaccct gctccaggcg atctgcaagc
     9661 gcatagtttc ttgatgattt tggaacggac gtgcggtatc tctggctaga aagatcgagc
     9721 aaatcaccgt gaacatttct cacctgatac tcataagagc gtgtcatttt tacctcaccg
     9781 attcaataac accacgcacc atgtcgcgca gatcaaccga aatctttgat ttgatcattt
     9841 cgtaatcatc gcggggctgg aatacgtttg taagattgac ctcaatctta tccgctactc
     9901 gggtgccgcc catcgctcca gatgccgcat cagctaactg agaagcaagc tcacctttca
     9961 gcgcaccaaa gtccttacca ggattattca tcggcatcgc gatctcgggc ccattatccc
    10021 cgatgatacc catgccgcct gctccaccca ttgctatacc accgtcgcca aacaaaggtg
    10081 gtggtggata ttgctgagcc gagattgatg aaacagttct cgcgccggca gcaatcacaa
    10141 gcgcagagag agtgcccgcc agaatagggc ccatgatcca tccagcgaat ggaatggtag
    10201 cggcaagcaa aacgcccgcc tgaattgcgt tcatcacgcc catcgcaacc gatgcgacag
    10261 cggctgccat ttgcgcacgc tttccaattt caaaagttgc tcggcctgcg ttccattcca
    10321 tgagctttgt ctgacgctca atcattgctt tcttgtccgc agtggcctgc gcacttgcgg
    10381 ccttctgatc ctcgatcatt tgaatgtgca aagcaagatc gtcttctcgt tgtttcgcag
    10441 tggtcgcgtg cacctttgct tcttcatcaa gctggctgtt tcgatttgta atatcgtttt
    10501 gcaaacgctt ctgagattcg ctgatcgctt gattcttctg cgcttcaatc tgcgcgagta
    10561 aatccgcttt gcgtgcttcc agttcagtgc cggtaactcc tgattgctca agatacgcaa
    10621 cctgcgcctg atactgatcg tcgagcctgc gaatttccgc cttcacttct tcatcaagct
    10681 cggcttttct ttgttgagcg tatgaatcct tcagcgcttt caggtcatcc tggtaacgtc
    10741 gctcctcatc cagaagcgca tctttctgct tttgcagtcc cttaatctgc gcatcgtaaa
    10801 atgcattgtc agccgccaat tgcagctcaa gcatcttcgc ggcaccacct gccatgaaat
    10861 ccagattctg ggtaaagttt gtaaggcgag tctgcgaaag tttctgcagg gattgcagat
    10921 actgcgtgaa cattccggag agagaagaga agacgccttt cactccttcg atgccaagag
    10981 cgatcgcttg catgcgtgac ttgaagtcca ttccagcaag acctgcggcc gcaaagccct
    11041 ccttcgcttt gattgcgttt tcgtaaatct tgtaaaactc gttcccgaat gtttcgaatg
    11101 tttccatcgg tccaatattg agtttgcgat ccgcagaatt gaaaagatta tccatcgcct
    11161 gcgcagcagg ggcagacttg gtaacgatgt ttttcaaagt agtgctaacg ttatccgaca
    11221 aagaactcag agacttcttc atgtccccag atgctttttt cacaatctcg gaagcctctc
    11281 cccatccctg gctgactttc tgaacaccct ccgccatcat caccatgttt ccggtcattg
    11341 agccagccat gaacttcatg aggccctcac caaccggagt gcgaagaact tgaatcaatg
    11401 catcaaacgc agtaaccaga acgttgatca cggttgcaag cgtgtagaaa gtgattaaca
    11461 tcggagccat cgcggccttc aatccagtag ctactagatc tacgttcttg aacgcgcccg
    11521 cgatggaatc aaaaatgcct ttgatcggag acagagtaga actgaagcga gaaaagatac
    11581 cgatgactga tgcaatcccg ctcgatacct gagactgaaa attccgaatc attgcggtca
    11641 atggcgcgaa gaaagcctga aattcctccc accttgactt catgtagatc acaccagccg
    11701 caacaagtgc gatcgcggtc acaaacagtc cgatcgggtt tgtgataaat gcagctttga
    11761 ttgcagcgct cacaagcgga gccacctgaa tcactccgta gagaacacca acaagagcgg
    11821 tggttgcaac aagcgccgtg ccgaacgtgt acacaaattc ctttgtgccc tgatccatgt
    11881 tgcgccacat ggaaatgaca tttttcacaa ttccgatcgc gccatccaga atctttgtgt
    11941 gattcaccca ttgaacgatc gacttcccaa tctccgtaga tactccggag actcccatct
    12001 tcagcttatc gaggttatcg ttaaattggt ctgcggcaat tgcatccgtt tttgaaaaca
    12061 cctgaccaag tgcatcggcc tcggtcatca actcgcgcag tgcagcaggc ccatccttca
    12121 acgcttcaat gaattgtccg cctgatttgc caagtgcgga ttgcgcaatt tccaatctgc
    12181 gaagcggatc gtttgttttt tcaatcacgc ctgcaagatc aagcatcaca tcggtctgac
    12241 tgcggagctt tccagtcgcg tccgttacag cgatacccat cgctacaagg ttcttttgtt
    12301 gctccggacg cgccatcttc gccataccag cggcgagtga ttctgcatct actccaccca
    12361 atttcatggc gtagctcatt gcagagaagc gatccgtcgc ctcaccagcg ctacgcgcga
    12421 gaacggtgtt cttatcgatt gtttctgcgg catgaatggc aagagccgcc atccctgtgg
    12481 cgactcctgc aactgcggca gacagggcaa gtgcggagtt cttcgcactg cccatgtcag
    12541 aaataaaatc cttagattct aatcggattc gcgtaacgag ttctttcatt tccgctcctc
    12601 cttagcctct ctgtactcct tctccatttt cgcttttttc ttggcttcga tccatgaacg
    12661 agttttgtct gccagcattt gaacccgtgt tttcaggccc atcagtatgc gacgttcatc
    12721 gtccagtgac tctaccattt tctttttgga ttccgcaact ttcccttctt catcaggaat
    12781 catttgcccg tattgcgcag cgctcattcg ctcaaggaac tcctcgattc gaagcgcttc
    12841 tgcaacttct gaccattctt ctacttcgtc tggccagaaa gtatcaaaca attctgatat
    12901 ggagggacgc gccaggatga gaatactcct gattgcggtt ctccttattt tttttcttct
    12961 tcagaagcct ccgcttttcc tgcgacaaca agcgtgagac tttcgatgat ttgagtcacg
    13021 taatccaaat cttccagatc ccatacccac ttcaaatcag tctcaggctt cacgtatttt
    13081 ccgagaacct tgattgcgcc atcggtgcta ctaagtttcc catccttcac ttctgcctga
    13141 atgcgattga tttctttgtc gcgttcgtct gcttccctaa cggacaagcg ccggatgata
    13201 agttttttaa ccttaccgtc cggcagctca acttccaaaa cccggtcgga gccgggcttc
    13261 tgtaatttta gtttcatgaa tctccacgga ttttccaggc cactggtctg cccagcggac
    13321 tcaggtgatc tgggtccggt ttcgcctgga acttcactgg gaaatacctc tgagtttttg
    13381 catcccatgt caagtccgct ttttcaatca ttgcagcggc ctgatagaaa tccacaatcc
    13441 tccacggatc gactgctacg atattcgtgt gtggatcgta ttccttgaat gtgatttgca
    13501 tgcggtggtc ttcggtgcga tacccgcgcg catctacaaa atgcactgca atcaggtttc
    13561 catcggtatc gaattcgccc tcaaggcatg gatggatcag aagcagagta tcccagtcag
    13621 gctttgcaag accgcactca accattgtct ggctggagct tgcaactgat tcgtcatcgc
    13681 gatctccgta gtccgcagaa cgcagaccaa tccagttcat ttcaatggac catttgattt
    13741 catttgtagg gccagcatcc ttattgatgc ctccggaagt ttccgtgtcc agaaacaatc
    13801 ttcctggacc gagcgcgtac ttgccgtctg ctagaatacc gggtgggttt gtaattgcgg
    13861 gcatagtgtc ctccgaccca ctcccccacc gtcacatatt ctatccggtc aattcaattt
    13921 tttaaggttt gaatgtgcgc accagaacgg tcttcggatg cacagtgggc tccatttcgg
    13981 cgaagccaaa aacatcctca atccattcgc gccactcctc cggagacagc aaagtcacat
    14041 gcgcattcct gccatcgggg agcttcgcgc gcgcgggctt ggtggcaatt gtgaaaatcg
    14101 cattacgact cacagaccgg atctcctcaa gaacgcggtc cacatgccgc atttcaatgt
    14161 gctccatcac gtcacaacaa agggctagat caaatgcgga accggtggga aaatccttat
    14221 actgaggaat cgccggatcg tagagataaa ttctccgctc gccatccctc cagaaatagt
    14281 tcccaaaacc ggcctgaccg catccgtaat caatgattga atttggttta tttgcgcgga
    14341 ccaggccggc gatctgctcc aaatgatcgg cagccgaaac accgtaacgc ttctggtgca
    14401 tctcctcata cattccgatc atatcgttgt aggacggagc atccttgatg aatgcagcaa
    14461 gcgtgatgtc cctacgccct gcttctatct ccgaattatt tgtgcacaaa agctgaaatc
    14521 cgttcatacg gaaaaactca atcaatccca gctcggtgaa atagtacagg tgctcccctg
    14581 gcttatagtg cggccatgat tcaatcccgg tgaatgatgg ctcatgaaat gttgggattg
    14641 cgacaaagac aaaagagcga tccggaatcc tgcgcagaaa tctgctcgga tcttccatgt
    14701 gctccagaac atcccacaaa gtgaatgcac ggaaccgcga gaaatcatcc gaatacttgc
    14761 cggccttctt cagcttggaa accgtttccg gaacaatatc gtatccgtaa gtgcgatcac
    14821 gcgactcaag aaaatccata gcgcccacgc cgacgtcgag cacttccact tcttccttca
    14881 cgtattctcg aacgatctga ttccttcctg cgagaatcgc gctcattcga tccggatttt
    14941 ctttatactt ccggcaggat tcaatgtatt cgtcagtata ctggatgcga tctttcgtcg
    15001 cctgataggc gattcccttc ttggggatta tcatcaggtc ccccgagtga tacgcctgga
    15061 gccttctcaa caggttgtcg ctcaattaca aatccatcct tcttcccgca cgcaatacac
    15121 tcaaattcct tatccatgct ttgcatccac atccaccggc acgctttgca gtacatatgt
    15181 ctcagcatga cttcatcaac tcctccagcg aacggtctgc cttccaattc gccggaaaaa
    15241 tctttttcaa caatttcaat tctttcaacg gacgatgccc gattggatca tccggattct
    15301 ttggagccca tgactcattc aggttatgct tattcgaagt gaagaaatca aacccagtaa
    15361 catacacgct tttggtttcg cactgcatca actcaagcaa gcaagcaaac ccggtggtag
    15421 gctgatggcc gggatacttg tcgtcttcat tctggaccag cgcgaataat tccatgaacc
    15481 tgctcaggct tggaacatat gtatcgcaga accagaatgc agcgcgcgcc gtgtaaacgt
    15541 accgatagtt tacgccatgc tgcttattgt tctgccggtg ccacggggat gaaaaaggaa
    15601 gcgcaccgtc tggcagcttg cacatgcaaa gcgatacgcc atcctccttc aattcattcg
    15661 ctgttttgcg catggacatc ccgtagaatg aataatgaac atccgttcga ctaccgactt
    15721 gagactcaaa cccgcgcatc ttgtaatttt ggatcctaat gattaaatca tgcgagtcga
    15781 tgtgctcccc ggaattcagc aaagcagtgg gaccggaacc aataaccgct attgattttc
    15841 ctttgaagcg ggcacgcatc tgatcaagcg aaatgaaatt agttttggtg cgcatgaacc
    15901 agtcccgact cccaagtttt gaccttctgc tcaatcattg aatcatcatc ccacaaaccg
    15961 atctccaaat ttccattcct tttgtgcctg agtaatagct tctcaggtga aatagaccgg
    16021 atgaattggt gctcggcact gcgtatgcaa tgaccgaaca aaacaatcaa aggtattttt
    16081 ctgcattctg caagagggat gatccaagag cactgtccta tgatgatgtc tgccgcacaa
    16141 aagtagctga tcgattcaga aatcgaagtt tttccagtca gatcctcatc agcgcgaata
    16201 tcatccgaat caccctgccc gatcttgatg agcttgtgat ccggcaaagc cttaatggca
    16261 gcacgaacgg aaggaatcga tgggatcgtt tcaaacccaa acccatctga acgccccatc
    16321 ggagcccgca agttctggta gacta
//
LOCUS       DAOQLC010000098        16204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1298_length_16204_cov_11.755031, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000098 DAOQLC010000000
VERSION     DAOQLC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1298_length_16204_cov_11.755031"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PL000_15540"
     CDS             <1..866
                     /locus_tag="PL000_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90347.1"
                     /translation="VVDMMFDNWPMAFQLTEGAKHAVQSPSGYYGFRVMRGFDDKGKV
                     LKPTPSDALFFTGHHVDHELVENIEVDCDDRLRRMKAKEPRRFLLTMGGAGAQRELFK
                     AIVEHVIPLVKQDKVTLFVNLGDHKNNWEWLQGELKDYKDLIQTHFTWEDTKEFTDSI
                     RKGPAKGLHVFLHDNTFHAVYATNYLMRVVDVMITKPSELAFYPVPKIFNARVGGHEM
                     WGAIRGAEIGDSTVEVRTIPQTLQAIDMLTDYDDLLTMFCDCIVKNKSIGIYDGAYKS
                     VELATGKKFKR"
     gene            complement(924..2207)
                     /locus_tag="PL000_15545"
     CDS             complement(924..2207)
                     /locus_tag="PL000_15545"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90348.1"
                     /translation="MRRPISEEPSKPVEPIMPSTPPPAEGGTTFVAMRHRNYQLYFGG
                     QLISNIGTWMQVIAQGWVVYQLGHSELTLGLVAFASAIPTLIISPWGGVVVDRVSRRN
                     LLMMTQAGAMLLAFALALLTFANVVKEWHVIVLAALLGVVNAFDAPARQAFVPEMVGK
                     RDLPNAIALNSMMFNSARVIGPAIAGLMLAAIGAAWCFTLNGISYFAVLAGLWWMKLP
                     PHRSTQHTTSPWQQMVSGVKYAAGYREISALILLSLVFSIFGVSYFTILPAFVEKILN
                     QGAIAYGWVNAASGLGAITGALVLASPLSNGKRGRILVITNLIFPILLIAFSFVRIYW
                     LSLVLAYGLGVGFMTQFTTINTLLQTRVDDAFRGRVMGLYTLTFFGFAPFGNLMIGAL
                     GEKMGLSFAMTLFACLSLILSRVVLTKTPEVQSLA"
     gene            2653..3429
                     /locus_tag="PL000_15550"
     CDS             2653..3429
                     /locus_tag="PL000_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90349.1"
                     /translation="MPASNILVIEDDTLVARTIERCLRGSEYHVQVVNTGVAGLKAAR
                     KKIPDLVLLDVMMPGMDGYTVCKEMREDALLKDVPVIFLTAKNKVEDKIVGLSVGADD
                     YLGKPFNVDELLLRVKAILRRTNPSGSRAVKSEKGLAGVVDTKTSQVTQTAPDPVIRI
                     GEYSLDTRTYMFSSPKKGKLRLTPIQYTLLYHLMTHPNKIFSPALLLDEVWDYPSDTG
                     SADLVRVHIKNIREAVEEDPKNPVFIKTVQGYGYTIQTEE"
     gene            3566..5578
                     /locus_tag="PL000_15555"
     CDS             3566..5578
                     /locus_tag="PL000_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90350.1"
                     /translation="MTSQPKVLVVDDEASIRTTIEGILSLGDYELHFAENGLQALAMA
                     IEIQPDIILLDVMMPGMSGFEVCKNIRAMQSLAEVPIVLVTALDDRESRMAGMKAGAD
                     DFVTKPFETQELFLRVQNMTRLNRYMRQTVYNSIELKFYESFVGEQNYSDPNATQVNA
                     ISNIRDYVEADAAMLVLFDLENPELATRKLLDSGASWRSENVFLVKNSRLCSSMTQTI
                     TMIDYSATTLGEVDPVLHDAATNPIRNIIVAPLNANNISFGAMIFINPLFDFEKDDRR
                     TRFLQLMVRGVANAAFALEHTRQLTISKAALEASQWEIINSRNTLRTFFDNIPSCVYI
                     IDRSYVIQIINSHRSERVSKAPQELVGSRCYEVLYGYNAPCPLCRVADAFMGMPSVRS
                     MREWQPKETFIQWEITTIPIFESSNTVNRVIVFDEDVTEKWVLEASLIETEKLASIGQ
                     LAANVAHEINNPLAAIIANAQLLLREQANADEDTKEALELIEAAGVRAAKIVGDLLKS
                     ARKEKREEFEEISLNETIMDAISIANFEIRNRDVTLKLELGENMPKILAHQNQLKGVW
                     INLIINALGAIEGQKGIITIMTRYEDREFRVAFSDNGKGIAPEHQEHIFEPFFTTKEA
                     SKGTGLGLSVSLQVIKEHHGTIDFETKPGKGTKFIIILPDVQQNGE"
     gene            complement(5606..6433)
                     /locus_tag="PL000_15560"
     CDS             complement(5606..6433)
                     /locus_tag="PL000_15560"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC90351.1"
                     /translation="MTESNPKLNAEGWEVVPKSPHKIKSPVMLTIPAGEFLMGTSDED
                     IKLLQIKESDWAYEWSDNDLFTAEQPRHSVSLPSYEIAQYPVTNAEYYTFIWDKGHRI
                     PRNWPGYTFLEGTDDHPVVGISKTDVEAYIQWLNEKTEVNFRLPTEAEWEYAARGDDG
                     RIFPWGNTFDPWRCNTVESMKKGTTPVGSYSPSGDSLFGAADMVGNVWEWTQSLFMPY
                     PYRPNTNREEVKPNGRYVVRGGAWYYSRKLARCAVREGVLQDHLSPSIGFRLARSIR"
     gene            complement(6430..7503)
                     /locus_tag="PL000_15565"
     CDS             complement(6430..7503)
                     /locus_tag="PL000_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90352.1"
                     /translation="MVITYIHSNHLADQIRVQVRCRNMVDAINRTGTHRAYLLDLASF
                     VQNTPLAQRLCLESDLLIIHRYLYGPILSAIQYWKARDKTVIADFDQAVNYLTEDMPA
                     FPFWHEGLPWEDSGFDGHSTIEPTPFEQFKWGLALVDAATVSSVRLVDDWSHVTNIFR
                     IPDFVNSHHYPLLNQNHGNEIWIGMGNCTGYESFKKSGLAEALENVCRKRPQVRLVLP
                     GSDESIGLLNIGASQLNVFFPHCFEDWVGILLELDIGLAPIHGNYSLRLDSYDLLEFM
                     IAKIPWIASETPALQKLSQLGQWTQNTPSAWEHAILNTVDQLSVKQRNAAGAPFLYAL
                     SQDIGANVTKVLQVYSIIMQGAR"
     gene            complement(7505..9157)
                     /locus_tag="PL000_15570"
     CDS             complement(7505..9157)
                     /locus_tag="PL000_15570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90353.1"
                     /translation="MQTELKVRKILGKTYLNENRLADALDVFSKILVDYPNDVETLHI
                     LGNFYLASGDGKTAQKIYQRALQLEPDNKAIQRQVRLAEEAGDTGTEEPIPTDLPAVT
                     RLLQRLSGVQTISENDIIRAATLLEKIVNSDNPAQLVALHLDEIDELLPALIELNIRQ
                     AQADGRPELAESLRNLQINIDHQMTVREGDELEQAVLDSVSGFNGNILFLNAEAGKQS
                     KRMNMLKTALKNLGCNVSEKRDFLALRDPKPDVVIANNPHLDPSLIESMSALSAAGVP
                     IILDLDIDFEKQPITHHEYNTKGLGTRTRSNAYTAALSVAAMVTVPSEIHADSLKKSV
                     NNIHVIPDGWSNQNKLWRKEAPARKTINIGWMGTSGELEDLVFIRRYIVRIIREFPNT
                     RVVVIGNPQAYRLFESLPENRRLYLPAVDDDELPYLLSQVDMLLVPLRNTPFNISRPD
                     TSLVEAGARGIPWVASSIPSFRRWQNGGVIAESMDEWHLNMRHLIMDEELRHKLGEQG
                     RSAAELREMNRIGKLWLDLIGEVISTEVSLPAAMPAYARLER"
     gene            9368..10039
                     /locus_tag="PL000_15575"
     CDS             9368..10039
                     /locus_tag="PL000_15575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC90354.1"
                     /translation="MTNTINQISRGYGLSALNTQTNQAESTSDFLSSFKDIISTMTMV
                     NGGMSTGQQLNSTINDLMSPLLMLILLEKLVELQVEDDSGSVNTTQETVQNVVSNQTE
                     KSVPSGRPVGGFLSQDYHPGHNGLDFGVVVGTPVETTMDGKVVYAGWNNQGYGNLVIV
                     ENGDYKTYYAHLSSIPVSVGDTVTAGATIGLSGNTGNSTGPHLHYEIRKNNVPIDPTS
                     ATLGG"
     gene            10051..10875
                     /locus_tag="PL000_15580"
     CDS             10051..10875
                     /locus_tag="PL000_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90355.1"
                     /translation="MLPMTKPSSASNMEIMALLRQLARDQAVVRMLNIYKGLPISYDT
                     YVYAVGASEIQMPSSKNHIACLYHQGETFLQGDTLPVVIRSKVTRLNLAKDFAILTNF
                     EIVKNDIGKRMQIRVEPDEPLVATIQFRGSAYEFLAPVADISANGASVYFDGDMFPSR
                     LAQPGNELTMSISLPDSAARKLRKAMPLPKIEGRKIASSLQTHLHGGQDRQGIVTAKG
                     KIIAARHETASKRYRLNTQLTFKDPHRMVLLQYISQRQTEIIHDLRILSEDLYHNK"
     gene            10993..12306
                     /locus_tag="PL000_15585"
     CDS             10993..12306
                     /locus_tag="PL000_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNC90356.1"
                     /translation="MIFPGPISISPINNTNGQSFDLRVFQRITAQVLSVTGTTAVLEV
                     EGHPVVAQLTSADQSALLSSQRTAQFVITQLGDNSVTLKLIKNEQTFPAGTAFGPDLA
                     ERFLENNNLPITTSNLVMTRALLRQHLPITPRTLKELQGALSEYDDWGEAEADLAAAI
                     KSAGLPLTGQSLALSARRPAPTAQAFSQLMATLAQAAKQNLPEDLLEQLRSNLQFLNS
                     LILDRGGDSSRMADQLKAWAEAFGRSLENILLSEIQNGAESISKNNLIELAKLQNLLE
                     QFGRQETARSLKEFLTDIRREQLMNVRSDSPSTTEWLEIGFALPKANKDFASARLRIA
                     RDGTAKAINPASTRLVLQVDLSPAETVEVDLSFAGKQIRSSVTSPNPAWCKQAQAELP
                     SLTDALAELGYTLTDFQVDVQMPQSFNRLQSGTGSIDLMTVNIEV"
     gene            12308..12616
                     /locus_tag="PL000_15590"
     CDS             12308..12616
                     /locus_tag="PL000_15590"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNC90357.1"
                     /translation="MGYKRFRVRPRSENESAPLRATALQYDSDRDKAPRVVASGQRKM
                     AEQILAEAKKHNISIYEDTALTAALAAVNPGEEIPPELYQVVAQVLAYVYRVTEKQGK
                     "
     gene            complement(13012..14271)
                     /locus_tag="PL000_15595"
     CDS             complement(13012..14271)
                     /locus_tag="PL000_15595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HNC90358.1"
                     /translation="MIRSMFTAISSLNLHQKYLDVVSNNLANANTTGYKSSRVLFQDQ
                     FAQMMAPGSAPSATQGGINPTQIGLGVGMGFVSPDFTQGTLQSTGRNLDLGVEGDGFF
                     VYGQDASRRYSREGSMGLDADGYLVNAATGLRTQGWMADATGAVDPNAPVDDIQIDTT
                     KSLARATENVALGGNLSSDTDGTGTATVSIAVYDSLGALQNASIRFTRGGVVGTPTNV
                     WTWQVMDTSVTPPVAGTGTGTITFDANGQVSTTTPPTVTTPATIPGSAGSTTPIPVTF
                     DFTKMTQLKATTSATVTSQDGLAAGSVTDVYISPNDGGVSLVYSNGLSEKVGQVALAR
                     FTNPAGLIQSEGTTYMSGLNSGEPEIGAASSGGRGTIASGHLEASNVDMAQEFTNMIL
                     AQRGFQASSRVITTSDEILQELVNLKR"
     gene            complement(14350..14778)
                     /locus_tag="PL000_15600"
     CDS             complement(14350..14778)
                     /locus_tag="PL000_15600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HNC90359.1"
                     /translation="MADPFHVQLGRVNGVGAQPATQASTVATNATTAESLSFLETLEN
                     VQRVHFSNHAQSRIQSREISLNSENVNRLSDAIDKAEKRGGKSSLVMVDDLAFIVNVQ
                     SRTVVTALDTTQRGEGVFTQIDSVVFADPAVEKSTIDVKG"
     gene            complement(14781..15197)
                     /locus_tag="PL000_15605"
     CDS             complement(14781..15197)
                     /locus_tag="PL000_15605"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNC90360.1"
                     /translation="MQVNNVLSAYQVTANNTANANPASNELDSGQFMQILLAQLTHQN
                     PLEPMDNAEMMSQFSQLNSLQELRGINTSMESVSVTSQTTYLTSLVGKTVKASRADGK
                     VIEGVVEGVATDVENPQVLVGDEVVDLIDILEIKAD"
     gene            complement(15227..>16204)
                     /locus_tag="PL000_15610"
     CDS             complement(15227..>16204)
                     /locus_tag="PL000_15610"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNC90361.1"
                     /translation="DVEIPATKVATKPVNVTKVEMVDFEVVEVNPQPTPVVTKELPKA
                     VSADIEMPVVKMATKPVNVAKAEAVELEAVEVKPQSTPVATKELSNVVSVGVEVSGDV
                     EVLSRFEVAGRFEGREDAVVFEQGTAFAEQSAEADSESALVKFDPEKKADVKEVEQES
                     VLDASGVVEQQKGAAEIKKMEKAAMKPLDLGAEVVEQITSQLKPHFKSGETSIRMQLN
                     PQDLGTIEVQMTHNAQGVSVSFITEQSGTGQLLESQAGQLRQSLKDAGVQLANLNINQ
                     HHQPSHEGGGFRQSQQFTQTPPRSVLQVEVDEGMQVQRAGSSSEIDYLI"
BASE COUNT         3956 a         4238 c         4064 g         3946 t
ORIGIN      
        1 acgtggtgga catgatgttc gataactggc ccatggcgtt ccaactcacc gaaggtgcca
       61 agcacgccgt tcaatctccc tcgggctatt atggtttccg tgtgatgcgc ggtttcgatg
      121 acaagggcaa agtcctcaag cccactccca gtgacgcact cttcttcacc ggtcaccacg
      181 tggaccatga actggtcgag aatatcgaag tagattgcga cgaccgcctc cgccgcatga
      241 aagccaagga accccgccgc ttcctgttga caatgggtgg ggcaggcgca cagcgtgaac
      301 tcttcaaagc catcgtggag catgtgattc cattggtcaa gcaggacaaa gtgaccctct
      361 tcgtcaacct cggcgaccac aagaacaatt gggaatggct gcaaggcgaa ctcaaagatt
      421 acaaagacct tattcaaacc cacttcactt gggaagatac aaaggaattt actgacagca
      481 tccgcaaagg acctgccaag ggattgcacg tcttcctgca cgataacacc ttccacgccg
      541 tctacgctac caactacctc atgcgcgtgg tggatgtgat gatcaccaaa cccagtgaac
      601 tcgccttcta ccccgttccg aagatcttca acgctcgcgt cggcggacat gaaatgtggg
      661 gcgccatccg cggcgcagag atcggcgaca gcactgtcga agtccgcacc attccgcaga
      721 ctttgcaagc cattgatatg cttacagatt atgatgacct gctcaccatg ttctgtgatt
      781 gcattgtcaa gaacaagagc attggcattt atgatggtgc ctacaagagt gtggagctcg
      841 ccaccggaaa gaaattcaaa cgataaacga tcagccccta aaggtttcca aaacctttag
      901 gggctttttt tgatcatcaa tctttacgcc aggctttgca cttcgggggt ctttgttaat
      961 accacccgtg aaaggatcag cgataggcag gcaaacagag tcattgcaaa gctcaacccc
     1021 atcttttcgc cgagcgcccc gatcatcaag tttccgaaag gcgcaaaccc gaaaaaggtt
     1081 aatgtgtaca agcccatgac ccgaccgcga aacgcatcgt ccacacgggt ttgtaacagg
     1141 gtgttgatgg tggtaaattg ggtcatgaac cccacaccca ggccatacgc cagcacaagc
     1201 gaaagccagt atatccgtac aaaggaaaat gcaatcaaca aaatggggaa gataagattg
     1261 gttatcacca ggattcttcc gcgcttgcca ttacttaatg ggctggccaa aaccagggcc
     1321 cccgtgatcg ctcccaaccc ggaagctgca tttacccatc catacgcaat tgcaccttga
     1381 tttaagatct tctctacaaa tgcaggcaga atggtgaaat atgaaactcc aaaaatgcta
     1441 aagaccagcg aaagcaaaat caaagcagag atctcccgat accccgccgc atatttcaca
     1501 ccgctgacca tttgttgcca tggcgaggtg gtatgttggg tactgcgatg cggaggcagt
     1561 ttcatccacc ataacccggc caataccgcg aaataagata tcccattcaa agtaaaacac
     1621 caggccgcac cgatggccgc cagcatcaac ccggcaatgg cagggccgat gacccttgca
     1681 ctgttgaaca tcatcgaatt caaggcgatg gcatttggga ggtctctttt tccaaccatt
     1741 tccggcacaa aggcctgcct cgccggggca tcaaaggcat tcaccacacc caacaaagcc
     1801 gccagaacga tcacatgcca ttccttcaca acatttgcaa aggtcagcaa agccagcgca
     1861 aacgccagga gcattgcccc agcctgtgtc atcatcaaaa gattgcggcg tgaaacacgg
     1921 tccaccacca cgccgcccca cggcgaaatg atcagcgtgg gaattgccga agcaaaagcg
     1981 accagtccca gggtcaattc ggaatgaccg agttgataca ccacccaccc ctgtgcaatc
     2041 acctgcatcc acgttccaat atttgaaatc aactgtccgc caaaatacag ttgatagttt
     2101 cggtgccgca tggctacaaa ggtcgtgccg ccctccgcag gcgggggggt actcggcatg
     2161 atcggttcaa caggtttgga cggttcttca gaaattggtc ttcgcatggt ttcaaagatg
     2221 gggattttat tggatgggat taagaatcga caagtgacat tgtatccctg ttttgacctt
     2281 gcccctcacc cgactctcgg tcttgactcg ccgcaagctg ccacaactta agtgagattc
     2341 cctccccgta aaaatcttta cgtaaaattt atgtgagttt caataacatt gcagccgcct
     2401 tcacgccata tgaccgtttt ttgaacggcg tataattcca cttgaaaatc gtcctttggt
     2461 tgaatttgtg tacccgacca acctgacaaa acttccctta attccgtgac atcccgctta
     2521 aatttttccc aaagaataga aatatgactc gaactggatt attatcaaga aagaagtcaa
     2581 ctgcctgccc cggcaatatg gttgttgaat tcattcattt cacccctcat tgatgacagg
     2641 aggatacata ccatgcccgc gtccaacata ttggttattg aagatgatac cctggttgcc
     2701 agaaccattg agcgttgcct gcgtggaagt gaatatcacg ttcaggtggt taacacgggg
     2761 gtggctggtt tgaaagctgc acggaagaag atccccgacc tggtgctgct cgatgtaatg
     2821 atgcccggca tggatgggta caccgtctgc aaggaaatgc gggaagatgc ccttctaaag
     2881 gatgtccctg tcattttttt gacggccaag aacaaggtcg aggataagat cgtgggtttg
     2941 agcgtcggcg ctgatgatta tcttggcaaa ccgttcaatg tggatgaatt actcctgcgt
     3001 gtgaaggcga tcctgcggcg gaccaatccc tctggttccc gcgctgtgaa atctgaaaaa
     3061 ggactggcag gggttgtgga tacaaagacc tcgcaagtta cgcagaccgc gccagaccct
     3121 gtcattcgca ttggcgagta ttcactggat acacggacct acatgttctc cagcccaaag
     3181 aaggggaaat tgcggctgac ccccatccaa tacactttgt tgtatcacct gatgacacac
     3241 ccgaacaaga tattttctcc cgccctcctg cttgatgaag tatgggatta cccctctgat
     3301 accggcagtg ccgatcttgt acgtgtgcat atcaagaaca tccgtgaagc cgtggaagaa
     3361 gacccaaaga acccggtgtt catcaagacc gttcaaggat acggctatac cattcaaacc
     3421 gaagaataaa aactatcgtc gttttctcgt cccggttgcc gattcataaa aagctcttga
     3481 ggctcgtcat ggacaaggaa caacggcttt tcttcacaag gatacaattt tccaagccca
     3541 ttgcaatcag ccaaggatga gaagcatgac cagccagcca aaagtattag ttgtggacga
     3601 cgaggcaagc ataagaacga caatcgaggg gatcctgtcg ctgggggatt acgaactgca
     3661 ttttgctgaa aatggattgc aggcacttgc catggcaatt gagattcagc ctgacattat
     3721 tttgttggat gtgatgatgc caggtatgag cgggtttgag gtctgtaaaa atatccgcgc
     3781 gatgcagagt ttggctgaag tgcccattgt cctcgtcacc gctttggatg accgcgaatc
     3841 acgcatggct ggtatgaagg ccggggcgga tgattttgta acaaaaccct tcgaaaccca
     3901 ggaattgttt cttcgtgttc aaaacatgac ccgtctgaac cgctacatgc ggcagaccgt
     3961 gtataactcg atcgagttga agttctatga atcgttcgtt ggggagcaga attacagcga
     4021 ccccaatgcc acccaggtga atgccatcag caatatccgc gattatgtgg aagccgacgc
     4081 ggccatgctg gtgctgttcg atcttgaaaa ccccgaattg gccacacgaa aactattgga
     4141 ttcaggcgcc tcctggagat ctgaaaatgt atttctggta aagaacagcc gattgtgcag
     4201 ttcgatgacc cagaccatta caatgatcga ttacagcgca accacacttg gcgaagtgga
     4261 ccctgttctt catgatgcag ccaccaaccc gatccgtaat atcattgtgg ctcctttgaa
     4321 tgccaataat atttcctttg gcgccatgat ctttatcaat cccctgttcg atttcgaaaa
     4381 agatgaccgg cggacccgct tcttgcaact catggtgaga ggtgtggcaa atgccgcatt
     4441 tgcgctcgaa cacacccgcc agttgaccat cagcaaggcc gcgcttgaag ccagccaatg
     4501 ggagatcatc aattcacgca acaccctgag gacatttttc gataacatcc ccagttgtgt
     4561 ctacatcatt gaccgttcct atgtcattca gatcatcaac agccatcgca gcgaaagggt
     4621 cagcaaagct cctcaggaac tcgttggcag cagatgttat gaagtgttat atgggtacaa
     4681 tgccccctgt cccctgtgcc gtgtggcaga tgcattcatg ggaatgcctt ctgtacgaag
     4741 catgcgtgaa tggcagccca aggaaacctt cattcaatgg gaaattacca ccattcccat
     4801 ttttgaaagc tcaaatacgg tcaatcgagt cattgtcttt gatgaggatg tgaccgaaaa
     4861 gtgggtgttg gaagcaagcc tgatcgaaac cgagaaactg gcttccatcg gtcagttggc
     4921 ggcgaatgtt gcccacgaga tcaataatcc gctggctgcc attatcgcca acgcccaact
     4981 gttactgcgt gaacaggcaa atgccgatga agatacaaag gaagccctgg agttgattga
     5041 agctgccgga gtgcgggccg ccaagatcgt gggcgacctt ctcaagagtg cccgcaagga
     5101 aaaaagggag gaattcgagg agatctcatt gaatgaaacg atcatggatg cgatctccat
     5161 cgcgaatttt gagatcagga acagggatgt cactctcaaa ttggagcttg gcgaaaatat
     5221 gccaaagata ctggctcacc aaaaccaact aaagggagtt tggatcaact tgatcattaa
     5281 tgctttgggg gccattgaag gtcaaaaggg aatcatcacc atcatgacgc gttatgaaga
     5341 tcgtgaattc cgtgtcgctt tttcagataa tggaaaggga attgctcccg aacatcagga
     5401 gcatatcttc gagcccttct ttaccaccaa ggaagccagc aagggaaccg ggctgggtct
     5461 ttccgtcagt cttcaggtca tcaaggaaca ccacggcacc attgactttg agaccaaacc
     5521 cggcaaaggc acgaaattca tcatcattct gccggatgtg cagcagaatg gagaatgaac
     5581 cctgctctac ctaaagtgac ttgtttcaac gaatggaacg ggcaaggcgg aatcctatgg
     5641 atggggaaag atggtcttgc agcacaccct cgcgcacagc acagcgcgcc agtttgcgtg
     5701 agtaatacca cgctcccccg cggaccacat acctgccatt tggctttact tcttccctgt
     5761 tggtattggg gcggtaagga taaggcatga acagcgactg ggtccactcc cacacatttc
     5821 caaccatatc cgctgcaccg aaaagactat cgccgctggg cgaataagac cccacgggcg
     5881 tggttccttt tttcatgctt tccacggtgt tgcatcgcca cgggtcaaag gtgttgcccc
     5941 acggaaaaat tcggccgtca tccccgcgtg ctgcatattc ccattccgcc tctgtgggca
     6001 ggcgaaagtt gacctcggtc ttttcattca accattgaat ataggcctca acatcggtct
     6061 tggaaatgcc cacaacaggg tggtcatcgg tgccttctaa aaaagtatac ccgggccagt
     6121 tgcgtgggat gcgatgaccc ttatcccaga tgaacgtgta atattctgca ttggtcacgg
     6181 gatattgcgc gatctcatag gaaggcagcg acacggaatg tctgggttgt tcggctgtga
     6241 atagatcgtt atcagaccat tcgtaagccc aatctgattc cttgatctgc aatagtttga
     6301 tatcttcgtc actggtgccc atcagaaatt cgcctgcagg gatggtgagc ataacgggtg
     6361 atttgatctt gtggggagat tttggaacga cctcccagcc ttcagcattg agtttggggt
     6421 ttgattctgt catcgggcac cttgcataat aatggaatag acctgcaata ccttggtgac
     6481 attcgcaccg atatcctggc taagtgcata caggaagggt gccccggctg cgtttctctg
     6541 cttcaccgag agctggtcta cggtatttaa aatggcatgt tcccatgccg acggtgtgtt
     6601 ctgtgtccac tgcccaagtt gtgataattt ttgaagggcg ggtgtttcac tggcgatcca
     6661 gggaattttt gcgatcatga attcaagcag gtcgtaggaa tcaaggcgca gactgtaatt
     6721 cccgtggatc ggggccagcc caatgtccaa ttcgagcaaa attcccaccc agtcttcaaa
     6781 gcaatgcgga aaaaacacat tcaattgact cgcgccgata ttcaataaac cgatagattc
     6841 atctgaaccg ggcaaaacca gcctgacctg cggacgtttg cggcagacat tctcaagtgc
     6901 ttctgcaagc ccgcttttct tgaaactttc atagcctgtg caatttccca taccgatcca
     6961 gatctcgttc ccgtgattct gattcaacag cgggtaatga tgcgagttca caaaatcggg
     7021 gatcctgaat atgtttgtca catgagacca atcatccacc agccgcaccg atgacaccgt
     7081 agctgcatcc accaatgcaa gcccccactt gaattgttca aagggtgttg gttcgatggt
     7141 cgagtggcca tcgaaccccg aatcctccca cggcaggccc tcatgccaaa atggaaaagc
     7201 tggcatgtct tcggtcaggt aattgaccgc ctgatcgaaa tccgcgatca cagttttgtc
     7261 gcgtgctttc caatactgaa tggcagacag gatcgggccg taaagataac ggtggatgat
     7321 cagcaggtct gattcaaggc aaagcctttg ggccagtggt gtattttgaa cgaaggatgc
     7381 caggtccagt aaataggccc gatgggtacc tgtacggttg atggcatcaa ccatgttgcg
     7441 gcatcgcacc tgcaccctga tctggtcagc cagatgattg gagtggatat atgtgatgac
     7501 cactttagcg ctccagtctt gcgtaggctg gcatggccgc gggcagggag acctcggtgc
     7561 tgatgacctc tccgatcaaa tccagccaca gttttccaat gcgattcatc tctctcaatt
     7621 ctgcggcaga ccgtccctgc tccccgagct tatggcggag ttcttcatcc atgatgaggt
     7681 gccgcatatt gagatgccac tcatccattg actctgcgat gacgccccca ttctgccagc
     7741 ggcgaaagga cgggattgaa gacgccaccc agggaatacc ccttgcacct gcttccacca
     7801 aactggtatc ggggcgggaa atgttgaatg gagtattgcg cagcggaacc agcagcatat
     7861 ccacctgact cagcaggtag ggcaattcat catcgtctac tgcgggcagg tagagacggc
     7921 ggttctccgg caggctttcg aacaaccgat atgcctgcgg attcccgatg accacaaccc
     7981 gtgtattggg aaattcgcgg atgatgcgca caatatagcg gcggatgaac accagatctt
     8041 ccagttcacc gcttgtcccc atccagccga tgttgatggt ttttcgcgcg ggggcttcct
     8101 tccgccaaag cttgttctgg ttggaccatc catctggaat gacatgaatg ttgttcacag
     8161 attttttcaa tgaatcagcg tgtatctcag aaggcacggt caccatcgcc gccactgaca
     8221 gggcagccgt gtaggcattg ctgcgggtgc gggttcccag gccttttgtg ttgtattcat
     8281 ggtgggtgat gggttgtttt tcgaaatcaa tatccagatc aaggatgatg gggacacccg
     8341 ctgccgacaa ggcggacatg ctttcaatga gtgacgggtc gaggtgcgga ttgttggcaa
     8401 tgaccacatc tggtttgggg tcgcggaggg cgagaaaatc ccttttctct gaaacgttgc
     8461 agccaaggtt tttcaaggct gttttgagca tgttcatgcg tttggattgt ttccctgctt
     8521 cggcattaag aaacaggatg ttgccattaa agccggaaac gctgtccaat acggcttgtt
     8581 cgagttcatc gccttcccgt acggtcattt gatgatctat gttgatctgc aggttgcgca
     8641 gggattcagc cagttcaggg cggccatctg cctgtgcctg acgaatgttc aattcgatca
     8701 aggctggcag gagttcatca atttcatcca ggtggagtgc caccagttgg gcggggttat
     8761 cgctgttgac gatcttctca agcagagttg ccgcgcggat gatgtcgttt tcgctgatgg
     8821 tttgaacacc gctgaggcgt tgcaacaggc gggttaccgc gggcaggtcg gtagggatgg
     8881 gttcttccgt ccctgtatca cctgcttctt cagccagcct cacctgacgt tgaatggctt
     8941 tgttatctgg ttccagttgc aaagcgcgtt gatagatctt ttgcgcggtt ttcccgtcgc
     9001 cgcttgccag gtaaaaattc ccgaggatgt gcagggtctc gacatcattc gggtaatcca
     9061 ccagaatctt gctgaagaca tccagcgcat ctgcaagacg gttctcgttg agataggttt
     9121 ttccaaggat ctttctgact tttaattctg tttgcatcgg cttcaccaat cgactctgcg
     9181 gcacgttttc tggttacggc ctcgttcctt ttttgttttg cccagtatag tttattgata
     9241 agtcttatcc tataaatcat ttctgaatat tgcgtaaaga agagataaac aatttatgtg
     9301 caaataatac ggattttatg cgtcgtttac tcacattctt gagcttcgat cttagaatga
     9361 aggcactatg acaaatacca tcaaccaaat ctcccgcggc tatggtcttt cggcattgaa
     9421 tacccagacc aatcaggcag agtccacttc tgattttcta tcctcattca aggacatcat
     9481 atccaccatg acgatggtga atgggggaat gtccactggg caacagctca actccacgat
     9541 caacgacctg atgtcgccgt tgcttatgct cattctgctc gaaaagctgg tggagcttca
     9601 ggtggaggat gacagcggct ctgtcaacac tacccaggag accgttcaaa atgttgtttc
     9661 gaatcaaacg gaaaaatctg ttccatcagg ccgtccggtg gggggctttc tgagtcagga
     9721 ctatcatccc ggtcacaatg ggttggattt tggggttgtc gttggcacac ccgtcgaaac
     9781 gaccatggat ggcaaggtgg tatatgcagg ctggaacaac cagggttatg gaaatttggt
     9841 catcgtggaa aatggggatt acaaaaccta ttacgcccat ctttccagca tccccgtatc
     9901 ggttggcgat actgtgaccg ctggtgccac catcggcctt tccgggaata cgggcaattc
     9961 cacaggtccg cacctgcatt atgagattcg caagaacaac gtgcccattg acccaacttc
    10021 agccaccctt ggcggataag gtattgtttc atgctgccaa tgaccaaacc atcttctgca
    10081 tcaaacatgg agatcatggc attgctgcga cagcttgcaa gagatcaggc tgttgtgcgg
    10141 atgttaaaca tttataaagg cctgcccatt tcgtatgata cctatgtcta tgcggttggt
    10201 gcttctgaga tccaaatgcc aagcagtaaa aatcacatcg cctgcctgta tcatcagggt
    10261 gaaacctttt tgcagggcga tacactccct gtggtcatcc gctcaaaagt gacgcgctta
    10321 aatctggcaa aagactttgc gatcctgaca aattttgaga tcgtcaaaaa tgatatcggc
    10381 aagcggatgc agatacgagt ggagcctgat gagccgctgg tggctaccat tcaattcagg
    10441 ggttcagcct atgaattcct agcaccggtc gccgatatct cggcaaatgg cgcaagcgtt
    10501 tatttcgatg gtgatatgtt tcccagccgt ttggctcaac ctggcaacga attgaccatg
    10561 tccatttcac tgcccgactc agcggcccgc aagctcagga aggcaatgcc gctgccaaag
    10621 atcgagggtc ggaagatcgc ctcatccctg caaacccatc tgcatggcgg ccaggatcgg
    10681 cagggaattg tcacagcgaa gggcaaaatc atcgcggccc gtcatgaaac ggcatcgaag
    10741 cggtatcgct tgaacaccca gttaaccttc aaagacccgc atcgcatggt gcttttgcaa
    10801 tacatttctc aacgccagac cgaaatcatt catgacctgc ggattttgtc tgaagacctt
    10861 tatcacaaca aatagagttc atagaaagac aaagaaagat ctgtacctgc ccagtgtgcc
    10921 aactggtttt cataaaacct gttgtttgta gtaaatggtt tggcaatacg gaatgaggcg
    10981 taagatgcat ttatgatttt tcccgggccc atttcgattt caccgatcaa caacacgaac
    11041 ggccaaagct ttgaccttcg tgtttttcag cgtatcacag cccaggtatt atccgtcact
    11101 ggcaccactg ccgtgctgga agtggaggga caccctgtgg tcgcccaact cacttctgcc
    11161 gatcaatcgg cgctgctctc atcacaacgc acggcacaat tcgtcatcac ccagttgggc
    11221 gataattctg tcacattgaa actcatcaaa aacgaacaga catttcctgc tggtactgcc
    11281 tttggtcctg atctggccga gcgctttctt gaaaacaaca acctgcccat caccacctca
    11341 aacttggtga tgacccgcgc cctcctccgt cagcatttgc cgatcacgcc gcgtacgctc
    11401 aaagaattgc agggcgcact ttcagaatac gacgattggg gtgaagcgga agcagacctg
    11461 gccgcagcga tcaaatccgc tggtcttccg ctgacgggac aaagcctcgc tctttccgcg
    11521 cgtcggcctg ctccaaccgc ccaggcattt tctcaactga tggcgactct agctcaagcc
    11581 gcaaagcaga atctgcccga agaccttctc gaacagttga gatcgaacct gcaatttttg
    11641 aattcgctga tccttgatcg gggcggagat tcttcccgaa tggctgacca actcaaggct
    11701 tgggcagagg cgttcggcag gtcgttggaa aacattctgt tgagtgagat ccaaaacgga
    11761 gccgaatcca tttcgaaaaa taatctgata gagcttgcga aattacagaa tttgctcgaa
    11821 caatttggaa ggcaggagac cgcccgatcg ctcaaggaat ttctgaccga tatccgccgc
    11881 gaacagttga tgaacgtgag atctgattcg ccgtcaacca ccgaatggct ggagatcgga
    11941 tttgcccttc caaaagcgaa caaggatttt gcgtcggcac gtttgcgaat cgcaagggat
    12001 ggcactgcca aagcgatcaa ccctgccagc acgcgccttg tccttcaagt ggatctttcc
    12061 cccgccgaaa ctgtggaagt ggacctttct tttgcaggga aacagatccg cagctcggtt
    12121 acgagcccca acccggcatg gtgcaaacag gcgcaggcag aactgccctc actgacagac
    12181 gcactcgccg aactcggtta caccctcaca gattttcaag tggatgtgca aatgccgcag
    12241 tctttcaaca gactgcaaag cggcacaggc agcattgact tgatgacagt taatattgag
    12301 gtgtgagatg ggatataaac gcttcagggt cagaccgcgc agtgaaaacg aatcggcgcc
    12361 tttgagggcg accgccttgc aatatgactc tgacagggat aaagctccgc gcgtagtagc
    12421 tagtggacag cgcaaaatgg ctgagcagat cctggccgaa gcaaagaaac acaatatttc
    12481 catttatgaa gataccgccc tgaccgctgc gcttgcggct gtgaatcccg gtgaagagat
    12541 tccacctgag ttgtatcaag tcgtggcgca ggtgttggct tatgtatatc gggtgacgga
    12601 gaaacagggg aagtaattcc gttttcaaaa ccgcggcagc agaaaagacc tggaattcaa
    12661 aacaaggaat gcacccgcca taacaggagg gtgtattttt ttcttctgat atctttattt
    12721 gacctcttgc ataagtttta agaaatgcca ttttgaacca cggagttttt tttaattgtt
    12781 ttctctgtgc ctccgtggct ccgtggttaa gctttcaggt tttgcaagag gtcattgcaa
    12841 aagtctgaaa agtccgcttt tcactacgaa gataccaagg cgcaaatttt gtgtctttga
    12901 gattttgtgg ttgcgttttc aaacacgctc cacacaaaga ctacagccag tttaaaacat
    12961 gactcgccca cgaaacttga tttcggggcg agtcatgttt tgttttacaa attatcgctt
    13021 caggttgacc agttcctgca ggatctcatc ggaggtggtg atcacacgag aggaggcctg
    13081 gaagccgcgc tgggcgagga tcatgttggt gaattcctgt gccatatcca cgttggaggc
    13141 ttcaaggtga ccggaggcga ttgtgccgcg tccgccgctg gaggcagcgc cgatctcggg
    13201 ttcgccggag ttaagcccgg acatgtaggt ggtgccttcg ctctggatca accctgcggg
    13261 gttcgtgaag cgtgccagag ccacctgccc aaccttttca gaaagaccat tcgagtaaac
    13321 cagtgacaca ccgccatcat tcggggagat gtacacatcg gtgacagaac cagccgcgag
    13381 gccgtcttga ctcgtaaccg tcgcgctggt ggtggccttc agttgagtca tcttggtgaa
    13441 atcaaaggtg accgggatcg gggtggtcga acctgccgaa ccggggatgg tcgccggggt
    13501 ggtgacggtc ggcggggtgg tggttgaaac ctgtccattt gcatcaaagg tgatcgtacc
    13561 ggtaccggtg ccagccacag gcggggttac cgaggtatcc atcacctgcc aggtccagac
    13621 gttcgtcgga gtgccaacca cgccgccgcg ggtgaagcgg atcgatgcat tctgcaaagc
    13681 gcccagcgag tcgtacacgg caatggaaac cgtggctgtg ccagtgccgt ctgtgtcaga
    13741 actcaggttg ccgccgagag ctacgttttc tgtggcacgg gcaagggatt tggtggtgtc
    13801 gatctgaata tcatccacag gggcgttcgg gtctacagcg ccagtggcat ctgccatcca
    13861 gccttgggtg cgcaaaccag tggccgcatt gacaaggtag ccatctgcat cgaggcccat
    13921 ggagccttca cgtgagtagc ggcggctggc atcctgcccg tagacgaaga agccatcacc
    13981 ttccacaccg agatcgaggt tgcggccggt gctttgcagg gtaccctgcg taaaatcggg
    14041 tgaaacgaat cccatgccaa cgccgaggcc gatctgggtg gggttgatcc caccctgtgt
    14101 ggcagaaggc gccgagcccg gagccatcat ctgggcaaac tgatcctgaa acagtacgcg
    14161 gctggatttg tagcccgtgg tattggcatt ggcaagattg ttggaaacga catcgaggta
    14221 tttctggtgc aggttcaaag aactgatagc ggtgaacatt gagcggatca tgatatttct
    14281 ccttttggtt gtgtttcagg cttcctccag gagggccagc ctgaattgaa tttgaatttg
    14341 aaaaacggac tatcctttta catcgatcgt ggatttctcc actgcggggt cggcaaatac
    14401 gacactgtcg atttgcgtaa atacaccttc gccgcgctgg gttgtatcca atgccgtgac
    14461 aacagtgcgg ctttggacgt tgacaatgaa ggccaggtca tccaccatca caagagatga
    14521 tttcccgccg cgcttctctg ccttgtctat ggcatctgaa aggcggttca cattctcgct
    14581 gttcaggctg atctcgcggc tttgaatgcg gctttgtgca tggttcgaaa aatgcacacg
    14641 ctgaacattt tcgagagttt ccagaaagct caggctttct gctgtggtgg cgttggttgc
    14701 gaccgtactg gcctgagttg caggctgtgc gccgacccca tttacccgtc cgagttgaac
    14761 gtgaaatgga tctgccatgg ttaatctgcc ttgatctcaa gaatatcgat caggtccaca
    14821 acctcatccc ccacaaggac ctgtggattc tccacatcgg tggcaacacc ctccaccacc
    14881 ccttcgatca ccttcccatc ggcgcggctg gctttcaccg tcttgccgac caggctggtc
    14941 aggtatgtgg tttggctggt gacagaaaca gactccatcg aagtgttgat gccgcgcaat
    15001 tcctgcaggg aattaagctg cgagaactgc gacatcatct ccgcgttgtc catcggctcc
    15061 agcggatttt ggtgagtcaa ctgcgccagc aggatctgca tgaactgacc tgaatcaagt
    15121 tcgttgctgg ccgggtttgc attggccgtg ttgttcgcgg tcacctgata agcgctcaaa
    15181 acattgttta cttgcatacg gtttctcctt ttctacaaaa tatttttcag atcaaatagt
    15241 cgatctcact ggatgagcca gcccgttgca cttgcatccc ctcatccacc tccacctgca
    15301 gaacgctccg cggcggggtc tgtgtaaact gctggctttg tctaaaacca cctccttcat
    15361 gactgggttg atgatgctgg ttgatgttca ggtttgcgag ctgcacacct gcatccttca
    15421 aagactggcg caactgcccc gcttgcgact caagcagctg cccggtacct gactgttccg
    15481 tgatgaagct cacgctcaca ccctgcgcgt tgtgcgtcat ctgtacttcg atcgtcccca
    15541 gatcctgcgg attcaattgc atgcgtatcg aagtctctcc gctcttgaaa tgcggcttca
    15601 actggctggt gatctgctca accacctctg cccccaggtc aagcggcttc atggcggcct
    15661 tctccatctt cttgatttcg gcggcgccct tttgctgctc aaccacaccc gacgcatcca
    15721 aaaccgattc ctgttcaacc tcttttacat cggccttttt ttcaggatcg aatttcacca
    15781 gagcggattc cgaatccgcc tcggcagatt gctccgcaaa tgcagttccc tgctcgaaaa
    15841 caaccgcatc ttctctaccc tcaaacctgc ccgccacctc aaaacgactc agcacctcaa
    15901 catcccctga cacttccaca cccaccgaaa ccacattgga caattccttt gtcgccacag
    15961 gtgttgactg aggttttact tcaacagctt cgagttcaac cgcttccgct ttcgctacgt
    16021 tgacaggctt tgtcgccatt ttcacgacag gcatttcaat atcagccgaa actgctttgg
    16081 gcaattcctt agtcaccaca ggtgttggct gggggttgac ttcaacaacc tcgaaatcaa
    16141 ccatttccac tttcgttaca ttgacaggtt ttgtcgccac ttttgtggct ggtatttcca
    16201 catc
//
LOCUS       DAOQLC010000099        16128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1310_length_16128_cov_23.060847, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000099 DAOQLC010000000
VERSION     DAOQLC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_16128_cov_23.060847"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..790)
                     /locus_tag="PL000_15615"
     CDS             complement(140..790)
                     /locus_tag="PL000_15615"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HNC90362.1"
                     /translation="MSTPVILGINLVILVALGAVAKFAKSEPSTKGGFNFNYTTSDLV
                     IMAVLGALAGVINTWMGNVWYAANTVSPIYGAALQGTFMWAYILAYFLVRKSGSMLII
                     GIIEASVEALLGNQAGLSTLGWGISQGLGAEIVMAFCMYSKFGWLALGLAGAGASQFG
                     TVWSFILYGWSSLTDYLIAAPINLISGFIFSGLLGYFLGTMIANTGLLRATRRGAE"
     gene            complement(854..2221)
                     /locus_tag="PL000_15620"
     CDS             complement(854..2221)
                     /locus_tag="PL000_15620"
                     /inference="COORDINATES: protein motif:HMM:NF017207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monomethylamine:corrinoid methyltransferase"
                     /protein_id="HNC90363.1"
                     /translation="MLNYLEILDRAHTGTYITEESWDLEKIAMTTKRLVKKYKLEWNK
                     EELVTDDAALSEAIWKAGYELAVEVGAYSRSTERIIQLSQDEIDNGIRNMPQEVVMGE
                     GKDARTLYARHLHDERAPLFFGGSPGTPIPERIFLANVMSYMQEPLIDLATCGTLVEV
                     DGREVRTGNPIEIVSTRRELQYMRQGLKRVGRAGMGMLAAQSSVSELGDLAAAHPDYL
                     RKCDSHLVPVLNELKMDHRNISRAVNSLEYGMVNASLPCVIVGGLGGGPAGSAVVNVA
                     SYLVANITCLADYHILHPIHVRHVATSTREVLWVINATAQAFARHAPSIIVADIYPKS
                     GAGTKELLYETAANAIVNAVSGGHLEGVGAADGNAPNCSGLEARLMAEVALATHRMKM
                     SLKDANQFVLQLLPKYEHVFTQDGNPGKSFDEVYDTVSVTPQDFWQKMYEEVKTELKE
                     MGLQL"
     gene            complement(2275..2931)
                     /locus_tag="PL000_15625"
     CDS             complement(2275..2931)
                     /locus_tag="PL000_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNC90364.1"
                     /translation="MPENEHLNAITAAIIEGETDDAVEATQSALSASIDPMEILNIGL
                     MNGADEVGQRFERGEYFLPELMMTGRALKASMEVVKPALLEKYSDSTDRVKGKVVAAT
                     VHTDIHDIGKNIVSSMLTASGFEVTDMGVDVPIKSIIDKAEEIEARIICLSALLTTSM
                     PFMKDLIQLLVARDIRHKYIVLVGGASVTESWANHIGADGTARNAADAVKLAREKIGM
                     "
     gene            complement(3012..4253)
                     /locus_tag="PL000_15630"
     CDS             complement(3012..4253)
                     /locus_tag="PL000_15630"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC90365.1"
                     /translation="MSNLSNSNWDVVIAGGGPAGFFAAIRCAELNPSLQVLILEKSSQ
                     TLGKVLISGGGRCNVTHACFDPAQLIQFYPRGGDALRGAFTRFQPRDTVEWFERRGVK
                     LKTEADGRMFPITDDSSTVANCLREAAKQARVRVQLGTSLQRVEKTSSGRFRLELRKD
                     AESLSIQTNRLLIAAGSDSKTREIVKSLGHSIAEPVPSLFTFNVKDKRIDGLAGIAVE
                     AVTLKMDSITTRGPLLITHWGFSGPAVLRCSAWGARILYEKKYASPLTVNWLGDYKTD
                     SALEVLQRNKEWHENARKKVTAHSAFSQIPIRLWKQLTEFIGEKNWADVSKAELRKLA
                     EELTAGTFMIQGKGQFKEEFVTCGGVNLKEVNFKTMQSRLVDGLFFAGEVLDIDGITG
                     GFNFQSAWTTGWLAGNSMTDL"
     gene            complement(4317..5270)
                     /locus_tag="PL000_15635"
     CDS             complement(4317..5270)
                     /locus_tag="PL000_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC90366.1"
                     /translation="MTSKTLPQSQHFTYHELAEGIWAAIVIPTGLAASNSGIVDLGDH
                     TLIFDTTLSPASAVELRKTAEHLTGRAVTYVLNSHWHQDHVFGNAIFAPETKIVASAR
                     TSEIIAEKIPSIMDEFKKHWPNQLKEWAESAKVAKDEAERLDYEDGVRFAQSIMDTFP
                     QLELRLPDQTFTDRVEFKGTKRTVEFITYGGGHTDSDAFLYIPAERIIFAGDLLATKF
                     QPGMSSGHPYEWLKILAKIKSIEPNQLVPGHGELATLEDISLIERYLNELLQMAEQNW
                     RAGGTAESAAALRPPVFIEGWENAEAFEINMKFLHEAVQKK"
     gene            complement(5401..5640)
                     /locus_tag="PL000_15640"
     CDS             complement(5401..5640)
                     /locus_tag="PL000_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90367.1"
                     /translation="MSSEEKQIVHRVVENFIRTGNATDEQVKVTCLPVNKTSFVEQTG
                     GDGRIILLDEYKLDGKVIWASYSARSGTVYLSLKK"
     gene            complement(5642..6139)
                     /locus_tag="PL000_15645"
     CDS             complement(5642..6139)
                     /locus_tag="PL000_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNC90368.1"
                     /translation="MNFQEGDPVVHWTYGFGQVVRLEERELAGAKTLYYAVQVRDLTV
                     WVPDDGKLESRLRPPTPETEFKKMINILSSPGKPLPEDRHERKTRLQEMQKNGGTEAL
                     CHIIRDLHTYQQVKSLNDNDQVILKQSQTALLGEWGFVFSISTAQAEIELHRVLAPKT
                     HEIKE"
     gene            complement(6160..6639)
                     /locus_tag="PL000_15650"
     CDS             complement(6160..6639)
                     /locus_tag="PL000_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90369.1"
                     /translation="MINLNISALIYQPIKKVFDFVCTPENDFQWQYGTLASTTLSTGA
                     ENIGTIFRSVGNLMGHRIQSTFEVTEFEPNKKYGFKSLSGPLLSQTVYTFEIDESSTK
                     VSISTQANVVNFFQSDEGILEKKMKKQLKENLAMLKTLLEVRRVTAPLNADTQPSKT"
     gene            complement(6658..7065)
                     /locus_tag="PL000_15655"
     CDS             complement(6658..7065)
                     /locus_tag="PL000_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90370.1"
                     /translation="MDKKNKNPLLAGLINVLIPGASQLYVNNDRARFFRSFLMGAGAF
                     VVAIMLGNLIQNTRGYSLPQGVCMGSALTLVAVILFISGLRVAGERNSQTDNAAYYKS
                     MRRDGYAAKQTAANKAQTEGSASEQKDAEPKQK"
     gene            7397..8422
                     /locus_tag="PL000_15660"
     CDS             7397..8422
                     /locus_tag="PL000_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC90371.1"
                     /translation="MRKTRKYATISDVAQHAGVSIATVSRVLTGNTPVIPETAERVRA
                     AIAELRFVPRTAARTLASRKTNTIGLILSEIGGAFFPHLLKGIEAEVRAAGYELLIHS
                     TQGMRQHQHRAVGEHNTDGLLVFTSSLELDELKRLHSMNFPLVLMHQTPPADLNIPVI
                     TIENKDGAEMLVGHLIEKHGRRRIVYMQGPEGHEDSHWRERGYREALEKRNIPFDPEL
                     VVDGAFDEEQACTTIQQMLMDGIEFDAVFAGDDDAAMGVYRALKLAGRIIPDDVAVVG
                     FDDIQFARYISPALTTIRAPIEEVGREAVRQLIHLLNGQQAQSLVLMRTELVIRESCG
                     CALSNRL"
     gene            8510..9883
                     /locus_tag="PL000_15665"
     CDS             8510..9883
                     /locus_tag="PL000_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC90372.1"
                     /translation="MKKNIFKLLAFLVLTSLLLTACGGGGAAPAEKAKVTIFVGFGTG
                     TDPDQVTAQEALAKKFNESHTDIEVEFLIVPVEESTERYLAMVAGGNAPQLVGPHGIS
                     TIAKFKDQWEDVTPYIEKDKYDMSDFYGPSTTLNKYPDLNAGLPLGLYPSFIFYNKDA
                     FDAAGLEYPTHDYADTSWNIDSMKEMALKMTLDANGNTPADSGFDPATIKQWGYADTW
                     TDFRGQMTMFGADTKGRPTSDDYKTAQVNSAEWLFGAQWLHDGVFKDHFIPDAAGQGA
                     IDAASGDPFGGGHVAMWYSHTWYMSEGLNDLPFKYDIAPLFYNQNGERISRIHADLFT
                     MPKTAKNKDAAWEVMKWLVAPEQIVDVCKVYGCLPARQSSQEAYLNYMKERYPGIDYD
                     VIFTAIDHLDNPNHESWVPEWGKVNDTLNNTQTTLYTDPEADVEALMNTTNEDVQKIL
                     DEYWASK"
     gene            9959..10924
                     /locus_tag="PL000_15670"
     CDS             9959..10924
                     /locus_tag="PL000_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC90373.1"
                     /translation="MKPANTISLARREALAGYAFIIPWIIGFLVFTIGPMLASLYFSF
                     TDYNIVDPPRWVGLENYVRVFSGIPEYLRTGDTRQLGDPIFWKSLQVTIYFASLALPL
                     NLLFSFLLAVLLNQKVPGVNIWRTIYFLPSIIAGVAVTLLWIRILNPRMGILNPFLQN
                     VLGVDNPPGWLQDPNWAIPALVIMSLWGVGGSMIIYLAGLQGIPTDLYDAAKVDGANA
                     WQRFWNVTFPMMTPVLFYNLVLGMIGTFQYFTEVYVATSGTGGPQRATLFYNLYLYQN
                     AFRYFDMGYASTLAWILFIIVLVLTALIFRSSEMWVYYEGQLRGK"
     gene            10924..11982
                     /locus_tag="PL000_15675"
     CDS             10924..11982
                     /locus_tag="PL000_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC90374.1"
                     /translation="MKRIIDLFSWLFKSIKGHEFLFKGITTLVLAAGAVVIMIPLMFM
                     ISTSLKDPGQLRSDANTLIPRKPRAVELNGEKFNLYQVTVDGVSLEMAMIKKAPGGMA
                     FFADPANPEELIELKLAEQTALYDIDIHWENYPEALNAMPFFRYLGNTLVVTFVGMAG
                     MLFSSSLVAYGFSRFRARWLNVLFLVLLSTIMLPSQVRLIPIYIFFQKLGWVNTLLPL
                     IVPQFFANAYDVFLLRQFFMTIPLELDDAAKIDGANPLQVLLYIILPQARPALVSVGI
                     FHFLYAWNDFYEPLIFLHSRDNWTLAVGLQTFDALYTINTHLIMAVSVLMIFPPILLF
                     FFSQKIFTQGVVFSGVKG"
     gene            12061..13383
                     /locus_tag="PL000_15680"
     CDS             12061..13383
                     /locus_tag="PL000_15680"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNC90375.1"
                     /translation="MTNYTFPHNFLWGAATAAYQVEGAWNEDGKGESIWDRFSHTPGK
                     ISDQTTGDVACDHYHRYEQDIALMRRLGLKAYRFSIAWTRVLPAGRGRINPKGIDFYD
                     RLIDSLCAANIEPFLTLYHWDLPQALQDEGGWENRDTGLAFADYSALMVKHLGDRVKY
                     WTTFNEPSVVAFDGNLSGEHAPGNRDPRIAYQVAHNLMVAHGLGVQAIRSADSKVQAG
                     IVLNLWMADPATDAPEDIAEARAAWDRSESLFLDPIFKGYYSPAVYDLVGENMPKLQD
                     GDMALISQELDYVGINFYSRNLVSATQEIDTVPGSEYTEMGWEVCAPAMRRMLVKINN
                     EYRLPPIYITENGAAFKDEVSADGKIHDPRRLDYLKQHFIQTRLAMQDGVDVRGYMVW
                     SLMDNFEWGHGFTKRFGLIRVDYETQQRTFKDSGDWYAEVIRTNQIIE"
     gene            13413..14429
                     /locus_tag="PL000_15685"
     CDS             13413..14429
                     /locus_tag="PL000_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90376.1"
                     /translation="MKPDTKPETGVSHFANGWPHHFARDLEDILAHHCTYIVHCFSEN
                     EMVFARKRTAQFFKMTRDAGLGCWANPWSVLGLFGGEQFSAFVPRNPDACQILSTGQR
                     APAACPSADETKTAMKLWIDMAIDFGADTIFWDEPHLYIPDWDDLHFASDDAFACFCP
                     RCRVSFQNRFGESMPETLTAEMRLFREDLMVNFLAEMIGYAKDKGAKNAITLLPVEDE
                     ANESLPWDRIADIRGLDIFGTDPYWYLHDKDCKTFVGEQMKKTMDICEPRGLTPHFWA
                     QGFGIPSGRENELETGFSLAVERGAQSIAIWGMHGNSAFDGASENPEKVWDVVGKTFG
                     KLRR"
     gene            14426..14983
                     /locus_tag="PL000_15690"
     CDS             14426..14983
                     /locus_tag="PL000_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90377.1"
                     /translation="MKIISLASDQLELIQQAAQILVDAFKDHSPEAWPTLADGLTEVD
                     EMLADDRILRVALDENENVIGWIGGIEQYDGHVWELHPLVVKPGVQGKGIGRALVQDF
                     EEQVRMRGALTVMLGTDDEDNRTSLSNTDLYEEPWKKINEIKNLKRHPFEFYQKMGYR
                     ITGVVPDANGAGKPDILMSKKIQSK"
     gene            14994..15881
                     /locus_tag="PL000_15695"
     CDS             14994..15881
                     /locus_tag="PL000_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC90378.1"
                     /translation="MEKLFGGIEGGGTKFNCAVGNGPENILAEARFATTTPAETIRQV
                     CDFFMSHVDQLQAIGFGSFGPFDTDPTSPTYGYITTTPKPHWGNTNVLGMLREKINLP
                     FAVDMDVAVAGLGEATWGASKNDTCSLYLTVGTGIGGGYIMNGKPLRGLTSLEMGHIR
                     LARDPKLDPFQGACPYHGDCFEGLAAGPAVEKRLGQKGETLPDDHPFWELEAGYIAQA
                     LVNYILCLSPQRIIIGGGVMQKDFMFPAVRRKTQELLGGYISHDVILNHIDEYIVPPA
                     LGGRSGVLGSIALAMNLNA"
     gene            15878..>16128
                     /locus_tag="PL000_15700"
     CDS             15878..>16128
                     /locus_tag="PL000_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90379.1"
                     /translation="MKKIPNLPIVDLMPFSEIDDKRPALLVTSAPAWNAVKNNLRGLN
                     ITHTIEVTEATTEYWDNLQLPITNHQIEIIYAVGGGLVA"
BASE COUNT         3753 a         4118 c         4129 g         4128 t
ORIGIN      
        1 tgacttgttc gaaacgaaca agcggttctt gattggattg attcaatgaa ggctctcaaa
       61 cattaaccac gaagggtcac aaagggtcac gaaggttttt cctttgtgac cctttattcc
      121 tttgtggttt aaaaaaatat tactctgccc cgcgccgtgt tgcgcgcaac agacctgtgt
      181 tggcgatcat ggtgccgagg aagtaaccaa gcagacccga gaagatgaag ccagagatga
      241 ggttgatggg ggcggcgatg aggtaatcgg tgagggatga ccagccgtag aggatgaagc
      301 tccacacggt gccgaactgt gaagcacccg cgccagcgag accaagagcc agccagccaa
      361 acttgctata catgcagaat gccatgacaa tttcagcgcc gagaccttgt gaaatgcccc
      421 agccgagtgt ggagagaccc gcctgattgc cgagcagcgc ttcaacactg gcttcgatga
      481 tgccgatgat gagcatggaa ccagacttgc ggacgaggaa gtaggcgagg atgtacgccc
      541 acataaaggt cccttgcagg gcggcgccgt agatcgggct gacggtgttg gcagcatacc
      601 agacgttgcc catccaggtg ttgatcacac cagcgagcgc gcccaacaca gccatgatga
      661 cgaggtcgga ggtggtgtag ttgaaattga atccaccttt ggtgctcggc tcagactttg
      721 cgaattttgc aactgcgccc agcgccacca gaataacgag attgatacca aggattacag
      781 gagtactcat gatttcattc ctcttttcaa aacgggattt ggttttgcaa cagggcttcc
      841 accctgacat gcactacagt tgtaaaccca tttctttcaa ttcggttttg acttcttcgt
      901 acatcttttg ccagaaatcc tgcggcgtga cggaaaccgt atcgtacact tcatcaaacg
      961 atttgcctgg gttgccatcc tgcgtgaaga catgctcgta tttcggcaga agttggagga
     1021 cgaactgatt cgcatccttg agcgacatct tcatgcgatg ggtcgccagc gccacctcag
     1081 ccatgagacg cgcttccaat cccgagcaat tgggtgcgtt cccatcggca gcgccaaccc
     1141 cttcgagatg tccgccactg accgcgttga caatggcatt cgcggcggtt tcgtagagca
     1201 gttcctttgt ccctgcgcct gatttcggat aaatatccgc aacgatgatg gatggcgcgt
     1261 ggcgggcaaa ggcttgcgct gtggcattga tgacccacag cacttcccgt gtggatgtgg
     1321 cgacatggcg cacatgaatg gggtggagga tgtgatagtc tgcaaggcag gtgatgttcg
     1381 ctacgagata cgaagcgaca ttcacgactg ccgagccagc aggtccgccg cccaacccgc
     1441 cgacgatgac acagggcagg gaagcattga ccatgccata ttccaatgaa ttgaccgcgc
     1501 gcgaaatatt gcggtgatcc attttgagtt cgttcagaac gggcacgagg tgcgagtcac
     1561 atttgcgcaa ataatcagga tgtgccgccg cgagatctcc caactctgaa accgagcttt
     1621 gcgctgccaa cattcccatg ccagcacgac cgacgcgttt caatccctga cgcatgtact
     1681 gcaattcgcg gcgagtggag acgatctcaa tggggttgcc cgtgcggact tcccgcccgt
     1741 ccacttcgac gagtgtgccg caagtggcga gatcgatcaa cggctcctgc atgtacgaca
     1801 tgacgttggc gaggaaaatg cgttcgggaa taggcgtccc tggcgaaccg ccgaagaaaa
     1861 gaggagcccg ctcatcgtga agatgacgcg catacaacgt ccgcgcatcc ttgccttcgc
     1921 ccataaccac ttcctgcggc atattgcgga tgccgttgtc gatttcatcc tgtgaaagct
     1981 gaatgatgcg ttcggtggag cggctgtacg cgcccacttc cactgccagt tcgtaacccg
     2041 ctttccagat cgcttcggag agtgctgcgt catccgtgac gagttcttcc ttgttccact
     2101 cgagtttgta ttttttcacc agacgcttgg tggtcatggc gatcttttcc agatcccagc
     2161 tttcttcggt gatgtaggtg cctgtatggg cgcggtcgag gatttcaaga tagttcagca
     2221 tgtgggtttc ctgttgataa ataaacagac gcacagaaag tgcgcctgtt gtgtttacat
     2281 tccaattttt tcgcgcgcca gcttaacagc atcggcggcg ttacgggcag tgccatcggc
     2341 accaatgtga ttcgcccagc tttctgtgac ggaagcaccg cccaccagca caatatattt
     2401 atggcggatg tcgcgggcca caagcaactg gatcaaatct ttcatgaacg gcatcgaagt
     2461 ggtgagcagc gccgagagac aaatgatgcg cgcttcgatc tcttcggcct tgtcaatgat
     2521 ggatttgatg gggacatcca cacccatgtc ggtcacttcg aagcccgagg cggtcagcat
     2581 ggaagaaacg atgttcttgc caatgtcatg aatatcggtg tgaaccgtcg ccgcgaccac
     2641 cttgccttta acacggtctg tgctgtcgga atatttttca agcagggcgg gctttaccac
     2701 ttccatcgaa gccttgagtg cgcgccctgt catcattaat tctggcagga agtattcgcc
     2761 gcgttcgaaa cgctgtccca cttcatcagc tccgttcatc aaccctatgt tgaggatctc
     2821 catcgggtca atgctggcac tcagggccga ttgggtggct tcgacggcat catctgtttc
     2881 cccttcgatg atggcggctg tgatcgcatt caaatgctcg ttctcaggca tgaagtacct
     2941 ccaaacttgt cttgggatgg gtggaatttt atgttagtta ttcgcatggc gcaagtgccg
     3001 ccatggatgc tttacaaatc tgtcattgaa ttgcccgcca gccagccagt tgtccatgca
     3061 gattggaagt tgaacccacc tgtaatgccg tcaatatcga gcacctcacc agcaaagaac
     3121 aaaccatcca caaggcggct ttgcatggtt ttgaaattca cttcctttaa gtttaccccg
     3181 ccgcaggtga caaattcttc tttgaattgt cccttgccct ggatcatgaa tgtgccagcg
     3241 gtcaattctt cggcaagttt ccgtaattcc gccttcgata cgtcggccca attcttttcg
     3301 ccgatgaatt cggtcaactg tttccataac cgaatcggga tctgtgagaa ggccgaatga
     3361 gctgtcactt ttttgcgggc attttcatgc cattccttgt ttcgctgtaa tacttccagc
     3421 gccgagtcgg ttttgtaatc gccgagccag ttgacagtta agggcgaagc gtattttttc
     3481 tcgtacaaaa tccgcgcacc ccaggccgag cagcgcagca cggcggggcc actaaatccc
     3541 cagtgggtga tgagcagcgg cccgcgtgtg gtgatcgaat ccattttcag ggtcacggct
     3601 tcaaccgcaa ttcccgcaag gccgtcgatc cttttgtctt tcacgttgaa agtgaataat
     3661 gatggcacag gctctgcaat ggaatgcccc agcgatttaa cgatctcacg cgttttggaa
     3721 tcgctgcctg cggcgatcaa taatctgttt gtctggatgg aaagactttc cgcatctttt
     3781 cttaactcaa gcctgaatct gcccgaggag gttttttcca cccgttgcag tgacgtgccc
     3841 aactgcaccc gcaccctggc ttgtttcgcc gcctcacgca gacagttcgc caccgtgctt
     3901 gaatcatcgg tgatgggaaa catgcgccca tcggcttcgg tttttaattt tacgccgcgt
     3961 ctttcgaacc attccactgt atccctgggt tggaagcgtg tgaaagcgcc gcgcaatgca
     4021 tccccgccgc gtggataaaa ttggatgagt tgagcagggt cgaaacaggc atgtgtgaca
     4081 ttgcaacgcc cgccgccaga gatgagcacc ttgccgagag tctggcttga tttttcgagg
     4141 atgagcacct gcagcgacgg atttaattca gcacagcgaa tcgcagcaaa gaaacccgct
     4201 ggcccgccgc ctgcaatgac tacatcccag ttggagttgg aaaggtttga cattttataa
     4261 aaactccatg tcagtataca ctgacatgga gtttttatgg ttcgtgaatg gaagggttac
     4321 tttttttgaa ctgcctcatg taggaatttc atgtttatct cgaaagcttc ggcgttttcc
     4381 cagccttcga tgaacaccgg aggccgtaag gcggcggcac tctcagcggt gccgccagcg
     4441 cgccaatttt gttctgccat ttgcaacaac tcgttgagat aacgctcgat caatgatata
     4501 tcttctagtg tagcaagttc gccgtgaccg ggtaccagtt ggtttggttc aatggatttg
     4561 atttttgcaa gaatcttgag ccattcgtac gggtgaccgc ttgacatacc aggttgaaat
     4621 tttgttgcca acaaatcgcc agcgaagatt atccgttcgg cgggtatgta taggaatgca
     4681 tcactatcgg tgtgcccgcc gccataggta atgaactcga cggtacgttt tgtccctttg
     4741 aattccactc gatctgtgaa ggtttgatct ggcaggcgta attccaactg cgggaaggta
     4801 tccatgatgg attgtgcaaa ccgcacacca tcttcataat ccaaccgctc ggcctcatct
     4861 tttgcgacct ttgcactttc ggcccattcc tttagttggt tgggccaatg cttcttgaat
     4921 tcatccataa tggacggaat cttctcggcg atgatctcgc ttgtgcgtgc agaggcaact
     4981 atttttgttt cgggagcgaa aatcgcatta ccaaagacat ggtcttgatg ccagtgactg
     5041 ttgagcacat aggttaccgc acgcccggta agatgctcgg cagttttacg caattcaaca
     5101 gccgatgcgg gcgagagggt ggtatcaaaa ataagggtgt ggtcgccaag gtccacgatc
     5161 cctgaattgc ttgccgccag gcctgtcgga atcacgatcg ccgcccagat tccctcagcc
     5221 agttcgtgat aggtaaagtg ctgtgattgg ggaagtgttt tagaagtcat gggagtgcct
     5281 caacggaacg agtatttgtg caagatttta cctattttat aaaccaccca taaatataaa
     5341 catccttcaa acgatcggaa tgaagtttcg gttgcttgaa ggaatatatt ttcgcctgat
     5401 ttattttttt aggctcaagt aaactgttcc actgcgggca ctgtaacttg cccagatcac
     5461 tttcccgtcc aatttgtatt catccagcaa aatgattcga ccatcgccgc cggtctgttc
     5521 tacaaaactg gtcttgttca ctggcaggca ggtcaccttg acctgttcat ctgtggcatt
     5581 tccggtgcgg atgaaatttt ccacaactct gtgtacgatc tgtttttctt ctgatgacat
     5641 attactcctt aatctcatgt gtctttggag caagcacgcg gtgtagttcg atctcggcct
     5701 gggcggtgga aatggagaaa acaaagcccc attccccgag cagggcggtt tgcgattgtt
     5761 tcagaatcac ctgatcgttg tcgttgagtg acttcacctg ttggtaggtg tgcagatcac
     5821 ggatgatatg acagagcgct tcagtgccgc cgtttttttg catctcttgc agccgggttt
     5881 ttcgttcatg acggtcttcc gggagcggtt tccccgggct tgagagaatg ttgatcattt
     5941 tcttgaattc ggtttccggg gtggggggac gtaaccggct ttccaatttt ccgtcgtctg
     6001 gcacccatac agtcaagtca cgtacctgaa ccgcatagta cagagtcttg gcccctgcca
     6061 attcccgttc ctccaaccga acgacctgac caaatccata tgtccagtga acgactgggt
     6121 cgccttcttg aaaattcatg tggttctcct gataaagggt taggtcttgg atggctgtgt
     6181 atctgcattg agcggagccg taacacggcg gacttccagc agggttttga gcattgcaag
     6241 gttttctttg agttgttttt tcatcttttt ttccagtatc ccctcatctg attggaaaaa
     6301 attgaccaca ttggcctgcg ttgagatgct caccttggta ctgctttcgt cgatctcgaa
     6361 tgtgtacact gtctgcgaaa gcaacggacc ggagagggat ttgaagccgt attttttgtt
     6421 tggctcaaat tccgtcactt caaatgtgct ttgaatccga tgtcccatca aattcccgac
     6481 actgcgaaag atcgtcccga tgttttctgc gcctgtggaa agtgtagtgg aggccagagt
     6541 gccatattgc cattgaaagt cattttcggg ggtgcaaacg aaatcaaaaa cctttttgat
     6601 gggttggtag atcagtgcgc tgatatttaa attgatcatt ccactctttt ccggggttta
     6661 tttttgcttg ggttctgcat ccttttgttc agatgccgaa ccttcagtct gtgccttgtt
     6721 tgcggcggtt tgttttgctg cgtaaccatc acgccgcatg gatttgtaat aggcggcatt
     6781 atcggtttga ctgttgcgtt cacctgccac tcttaatccg ctgataaaca agatgaccgc
     6841 tacgagtgtg agcgcactgc ccatgcatac tccctgtggc agggaatacc cacgtgtgtt
     6901 ttgaatcaaa tttcccaaca tgatcgcaac gacaaaggct cctgctccca tcagaaatga
     6961 acggaagaat ctggctctgt cattgttcac atataactga cttgccccgg ggattaaaac
     7021 atttatcaaa cccgccagaa gggggttttt atttttctta tccataaatt tctccttttc
     7081 atggagagaa gacgttccac agatccccct tccagatccc atgatctctc tctgtcattg
     7141 tagcacgttc acttgcgaat tgttcgagca attttgtatg ccaacctgca gtagccattg
     7201 ccttgaatga tcggcgataa gcagagcttc ctccggttca agcattgcgc agtggagttt
     7261 ttcgcgggtg agattctatt gtttttgtaa tcagtttggg ggtgggagaa gaagcgaatt
     7321 tttagtcaat ttgttgacat gatgggtgag tctcgttata ctatgaaagc gttttctgaa
     7381 aacgctttca tagtccatga gaaaaactag aaaatacgcc acaatttccg atgtagctca
     7441 acatgcgggg gtatccattg ccacggtgag ccgtgtattg acgggaaata cccccgtgat
     7501 accggaaacc gctgagcgcg tgcgtgctgc gattgctgag ctgcgttttg tcccacgcac
     7561 ggcggcgcgt acccttgcca gccgaaaaac gaacacgatc ggtttgattc tttccgaaat
     7621 cggcggcgcg ttttttcctc atttgttgaa aggcattgag gcggaagtgc gtgcagcggg
     7681 ctacgaattg cttattcact ccactcaggg aatgcggcag caccaacatc gtgcagtggg
     7741 ggagcataat accgatggcc tgttggtgtt cacctccagc ctcgaacttg acgaactcaa
     7801 acgtctgcac agcatgaatt ttccgcttgt gttgatgcac caaaccccac ccgctgattt
     7861 gaatatccca gtcattacca ttgaaaacaa agacggcgct gaaatgctgg tcggtcacct
     7921 gatcgaaaag catggtcggc gccgcatcgt ttatatgcag gggcctgagg gccatgaaga
     7981 ttcgcactgg cgcgaacgcg ggtatcgtga ggcattggaa aaacgtaata tcccattcga
     8041 ccctgaactg gtggtggatg gggcttttga tgaagaacag gcctgcacca ccattcagca
     8101 aatgcttatg gatggaattg aatttgatgc ggtcttcgcc ggggatgacg atgcagcaat
     8161 gggtgtgtat cgcgccttga aactggccgg ccgcatcatc cctgacgatg tggcggttgt
     8221 cggtttcgat gacattcaat ttgcccgata catctccccc gcgttgacga cgatccgcgc
     8281 gccgatcgaa gaagtggggc gtgaagctgt ccgccagctg atccacttgt tgaatgggca
     8341 gcaggcccaa agtttggttt tgatgcgcac agaattggtc atccgtgaat cgtgcgggtg
     8401 tgcgctttcc aatcgtttat aaagacgggg aggtgttgtc gaagaagata caggatcaca
     8461 aggtcaaaat gaaaaccatc cattccatta atcacccctg gaggagcaaa tgaaaaaaaa
     8521 tatattcaaa ttgctggctt ttctcgtatt gacatcgctt ttgttgacag cctgtggcgg
     8581 aggcggagcc gcgcccgctg aaaaagccaa ggtgaccatc tttgtgggct ttggtaccgg
     8641 cactgaccct gatcaggtca ctgcacaaga ggcacttgca aagaaattca acgaatcgca
     8701 caccgatatc gaagtggaat tcctgatcgt tcctgtggaa gaatccactg aacgttattt
     8761 ggcgatggtg gctggcggaa atgcgccgca attggtgggt ccgcatggaa tttcaaccat
     8821 tgcgaagttc aaggatcaat gggaggatgt gactccctac atcgaaaaag acaaatacga
     8881 tatgagcgat ttctatggtc cttcgaccac gctcaacaag tatccagatt tgaatgcggg
     8941 tttgccgctg gggttgtacc cctccttcat tttctataac aaggatgcct tcgatgcggc
     9001 tgggttggaa taccccaccc acgattatgc cgatacttca tggaatatcg attccatgaa
     9061 agagatggcg ctcaagatga ccctggatgc gaacggaaac acccctgctg attctggctt
     9121 tgatcctgcc accatcaaac aatgggggta tgctgataca tggaccgatt tccgcgggca
     9181 gatgacaatg tttggcgctg acaccaaggg tcgcccgacc agtgacgatt acaagaccgc
     9241 tcaggtcaat tcggcagagt ggctgtttgg tgcccagtgg ctgcatgatg gcgtattcaa
     9301 ggatcacttc attcccgatg cagcgggtca gggcgcgatt gatgcagctt ctggtgaccc
     9361 gtttggcggt ggtcatgtgg ccatgtggta cagccatacc tggtacatgt ctgaaggttt
     9421 gaatgacctg ccgttcaaat atgatattgc gccattgttc tataaccaaa atggcgagcg
     9481 catttcccgc attcatgccg acctgttcac catgcccaag actgccaaga acaaggacgc
     9541 cgcctgggaa gtgatgaagt ggttggtggc tcccgaacag atcgtggacg tgtgtaaggt
     9601 gtatggatgt ctgcctgcgc gccagtcttc acaagaggcg tacctcaatt acatgaagga
     9661 acgctaccca ggcattgact atgatgtcat cttcaccgcc attgatcacc tcgataaccc
     9721 caaccatgag tcgtgggtgc ctgaatgggg caaggtgaat gataccttga acaataccca
     9781 gaccacactc tacaccgatc ccgaagcaga tgtggaagcc ctgatgaaca ccacgaacga
     9841 ggatgtgcag aagatcctcg atgaatattg ggcaagcaaa tagattgata tgactggatg
     9901 gaggggcaac cctccatcca gcttgaaatt attttttagt gtttttttct ggaggcgcat
     9961 gaaaccagca aataccatca gcctcgcccg ccgcgaagcc ctggccgggt atgcttttat
    10021 cattccgtgg atcatcggct ttttggtttt caccatcggc ccaatgcttg catcgttgta
    10081 tttttccttt accgattaca acattgttga cccgccacgc tgggtgggac tggagaatta
    10141 tgtccgcgtg ttctctggaa ttcccgaata tctaagaaca ggtgacaccc gccagttggg
    10201 cgacccgatc ttctggaagt ctttgcaggt gacgatatat tttgcttcgc tggccttgcc
    10261 tctgaatctg ctcttcagct ttttgctggc agtgttgttg aatcaaaagg tacccggggt
    10321 gaatatctgg cggaccattt attttctgcc ttccatcatt gccggggtgg cggttaccct
    10381 gttgtggatt cgcatcctca acccgcgcat gggaattttg aatccgtttt tgcagaatgt
    10441 gcttggggtg gataatcccc cgggctggct gcaagatcca aattgggcca tccctgcatt
    10501 ggtcatcatg agcctgtggg gggtgggtgg gtcgatgatc atttatctgg ctgggttgca
    10561 gggtattccc accgacctgt acgatgccgc caaggtggat ggagcaaacg cctggcaaag
    10621 gttttggaac gtcacttttc cgatgatgac ccctgtcctt ttttataacc ttgttctggg
    10681 tatgatcggc acctttcagt atttcacgga ggtctatgtc gccaccagcg gcactggtgg
    10741 accgcagcgc gccacacttt tctacaatct atacctgtat caaaatgctt ttcgctattt
    10801 tgatatggga tacgcctcca cgctggcttg gatcctgttt atcatcgtgc tcgtgctgac
    10861 cgcacttatt ttccgctctt cggagatgtg ggtatattat gaaggccaat tgagaggcaa
    10921 ataatgaagc gaataattga tcttttttcc tggcttttca agagtatcaa ggggcatgaa
    10981 ttcctgttca aaggcatcac tactttggtt cttgcggcgg gagcggtggt gatcatgatc
    11041 ccgctgatgt tcatgatttc cacttctctg aaagaccccg gtcagctgcg ttcagatgca
    11101 aataccctga tcccgcgcaa accgcgggcc gttgaactca acggcgaaaa atttaacctg
    11161 tatcaggtga ccgttgatgg cgtttcgctc gaaatggcaa tgatcaaaaa agcgccgggt
    11221 ggcatggcct tttttgcaga tccagccaac cccgaagagt tgatcgagct taagctggcc
    11281 gaacaaaccg ccctctatga cattgatatc cattgggaga actatccaga agcgttgaat
    11341 gccatgccct ttttccgcta tctggggaat acgctcgttg ttacctttgt aggcatggct
    11401 ggcatgttgt tttccagttc actggtggct tacgggttca gccgtttccg tgcgcgttgg
    11461 ttgaatgtgc ttttcctggt gttgttatca accatcatgc tgccctcaca agtgcgcctg
    11521 attcccattt acatcttttt ccagaaactt ggctgggtaa atacgctttt gccattgatc
    11581 gtgccgcagt tctttgcgaa tgcctatgac gtatttttgc ttcgccagtt tttcatgacc
    11641 attccgcttg aattggacga tgccgccaag atcgatggag cgaatccttt gcaggtattg
    11701 ttgtacatca tcctgccgca ggcacgccct gcactggttt ctgtgggtat cttccatttc
    11761 ctctacgcgt ggaatgattt ttacgaaccg cttatcttcc tgcattcccg tgataactgg
    11821 actttggccg tgggtttgca aacttttgat gccctctaca cgatcaatac acaccttatc
    11881 atggctgtgt ctgttttgat gatcttcccg ccgatcctgc tgtttttctt cagccaaaag
    11941 atatttacac aaggtgtggt cttcagcggc gtaaaaggat aaatttacct ggccgaaaac
    12001 catcaccgta tacatcactc tgaacatgga cgaaaacaat ccacctgaaa cctgaatcct
    12061 atgacaaact ataccttccc acacaatttc ctttggggtg cggcaaccgc tgcgtatcaa
    12121 gtcgaaggcg cctggaacga agatggcaaa ggcgaatcca tttgggaccg cttcagtcac
    12181 acccccggca agatctcaga ccaaaccacg ggggatgtcg cctgcgatca ttatcaccgt
    12241 tatgaacagg atatcgccct catgcgccga cttggcctca aagcctatcg tttctccatc
    12301 gcatggacac gcgtcctgcc agccgggcgc gggcgtatca acccgaaggg aattgatttt
    12361 tatgaccgcc tcattgattc gctttgtgcg gctaatatcg aaccctttct cacactctat
    12421 cactgggact tgccccaggc tttgcaggat gaaggcggct gggagaatcg cgacaccggc
    12481 cttgcctttg cagattattc cgccttgatg gtgaaacacc ttggcgaccg cgtgaagtat
    12541 tggacaacgt ttaacgagcc cagtgtcgtc gcgtttgatg ggaatctttc cggggaacat
    12601 gctccgggga atcgagatcc gcgcattgcc tatcaggtgg cccataacct catggtcgca
    12661 catggtctgg gcgtgcaagc catccgcagt gctgattcaa aagtgcaggc gggaattgtc
    12721 ctcaatttat ggatggcaga cccagccacc gatgcgcccg aagacatcgc cgaagccagg
    12781 gcagcatggg atcgcagtga atcccttttc cttgacccga tcttcaaagg atactactct
    12841 cccgcagtgt atgatctggt gggggagaac atgccaaaac tgcaagacgg cgacatggcc
    12901 ttgatctcac aggaactcga ttatgtgggc atcaattttt actcgcgcaa tctcgtcagc
    12961 gccacacagg aaattgacac cgtacctggc tcggaataca ccgaaatggg atgggaagtt
    13021 tgtgcgcccg caatgcgcag gatgctcgtg aagatcaata atgaatatcg tctgccgccg
    13081 atctatatca ccgaaaatgg tgcggcattc aaagatgaag tcagcgccga tggaaagatc
    13141 cacgacccgc gcaggctcga ctatttgaaa cagcatttca tacaaacccg cctcgccatg
    13201 caggatggcg tggatgtgcg cggttacatg gtctggtccc tgatggataa cttcgaatgg
    13261 ggtcacggct ttaccaaacg attcggcctc atccgcgtgg attacgaaac ccaacagcgc
    13321 actttcaaag actcgggtga ttggtacgcc gaagtgatac gcaccaatca gatcatcgaa
    13381 tagtttaaga aacaaaacct ggaacctgaa acttgaaacc cgataccaaa cctgaaaccg
    13441 gcgtttccca ttttgccaac ggctggccgc accactttgc gcgcgatctt gaagatattc
    13501 ttgcacatca ctgcacgtac atcgtgcatt gtttcagcga gaacgaaatg gtctttgcgc
    13561 gcaaacgcac tgcacaattt tttaaaatga ctcgcgatgc gggccttggc tgctgggcga
    13621 atccctggtc tgtgcttggg ctgttcggcg gcgaacaatt ttctgcattc gtgccgcgca
    13681 accccgatgc ctgccagatc ctttccacgg gtcagcgtgc gcccgccgcc tgcccatcag
    13741 ccgatgaaac caaaaccgcc atgaaactct ggatcgatat ggcgattgac ttcggtgccg
    13801 ataccatctt ctgggatgaa ccgcacctct acataccaga ttgggatgac ctgcacttcg
    13861 cctcagacga cgcctttgcc tgcttctgtc cgagatgtag agtctcgttc cagaatcgct
    13921 tcggtgaatc catgcccgaa acattgaccg ccgaaatgcg cctcttccgc gaagacctga
    13981 tggtgaattt cctcgccgag atgatcggct atgccaaaga caaaggcgca aagaatgcca
    14041 tcacgctttt gcctgttgaa gacgaagcca acgaatcact cccatgggat cgaatcgccg
    14101 acatccgtgg gctcgacatc ttcggtactg acccgtattg gtatttgcac gataaagatt
    14161 gcaaaacctt cgtcggtgag cagatgaaaa agacgatgga catttgcgaa cctcgtgggc
    14221 tgactccgca cttttgggcg cagggttttg gaatcccatc gggcagggaa aatgaactcg
    14281 aaacaggatt cagcctcgcc gtcgaaagag gcgcgcaatc catcgccatt tggggcatgc
    14341 acggaaattc cgcctttgat ggtgcttccg agaatcctga aaaagtttgg gatgtggttg
    14401 gcaaaacctt tggaaaattg agaagatgaa gatcatttca ctggcctccg atcaacttga
    14461 attgatccag caagctgcac agattcttgt ggatgccttc aaagaccatt ctcctgaagc
    14521 ctggcccacg ttagccgatg gcctgaccga agtggatgaa atgctcgctg atgatcgcat
    14581 tctgcgcgtt gcactcgatg aaaacgaaaa tgtcatcggt tggatcggcg gcatcgaaca
    14641 atacgacggg cacgtctggg aattgcatcc gctggttgtg aagcctgggg tgcagggcaa
    14701 aggcattggc cgcgcattgg ttcaggattt tgaagaacag gttcgaatgc gcggcgcact
    14761 caccgttatg ctgggcacag atgacgaaga taacaggact tccctgtcga acacggatct
    14821 ctacgaggaa ccctggaaaa agatcaacga gatcaaaaac ctgaaaagac acccctttga
    14881 gttttaccaa aaaatggggt atcgtatcac aggtgttgtg ccagatgcca acggcgctgg
    14941 caaacctgat attttgatgt ctaaaaaaat acaatcgaaa taggatcaat aaaatggaaa
    15001 aactttttgg cggcatcgag ggcggcggaa cgaaattcaa ctgtgctgtg ggcaatggcc
    15061 ccgaaaacat tcttgctgaa gcgcgctttg caaccaccac accagccgaa acgatcaggc
    15121 aggtgtgtga ttttttcatg tcccatgtag atcaacttca ggcgatcggc ttcggttcgt
    15181 tcggcccatt tgatacggac cccacttcac ccacctatgg gtacattacc accacaccga
    15241 aaccgcattg gggcaatacc aacgtgcttg gcatgctgcg cgagaagatc aacctgccct
    15301 tcgcagtgga tatggatgtg gccgtggcag ggttgggaga agcaacctgg ggcgccagca
    15361 aaaatgatac ctgctctctt tacctcacgg tgggaacagg gatcggcggc ggctacatca
    15421 tgaacggcaa acccctgcgc ggactcacca gcctcgaaat gggacacatc cgcctcgccc
    15481 gtgacccgaa actggatccg tttcaagggg catgtccgta ccacggagat tgcttcgaag
    15541 gtctcgctgc aggacccgca gtcgaaaaac gcctgggtca aaaaggtgaa accctgccag
    15601 acgatcatcc cttctgggag ttggaagcag gttatatcgc acaggccctg gtcaattaca
    15661 ttctctgcct ctcgccgcaa cggatcatca tcggtggcgg cgtgatgcaa aaggacttca
    15721 tgttccccgc tgtgcgcaga aagacacagg aactattggg cggctatatt tctcacgatg
    15781 tgattctcaa ccacattgac gaatacattg tcccacccgc gctgggcgga cgttcgggag
    15841 tattggggag catcgccctt gcaatgaatt tgaacgcatg aaaaagattc caaacctccc
    15901 cattgtcgat ctcatgcctt tctccgagat tgatgataaa cgtcccgcat tgctcgtcac
    15961 ttctgcgcct gcatggaacg ctgtcaaaaa caatttgcgc gggctgaaca tcacccacac
    16021 aattgaagtc accgaagcaa ccaccgaata ttgggacaat ctccaattac caattaccaa
    16081 tcaccaaatt gaaatcatct acgccgtggg tggcggactt gttgctga
//
LOCUS       DAOQLC010000100        15917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1328_length_15917_cov_26.023515, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000100 DAOQLC010000000
VERSION     DAOQLC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1328_length_15917_cov_26.023515"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1625
                     /locus_tag="PL000_15705"
     CDS             <1..1625
                     /locus_tag="PL000_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90380.1"
                     /translation="NDANYAGSANGTLVISKANQNISFTTAAPVGALVGGPVYTPAAS
                     STSSLLVSITIDPSSAAVCSISGGDISFLSTGTCKVNANQAGDSNYNPASQVQQSFGI
                     GLGNQVISMTSSVPAAAVVDGPVYTPSATGGASGNPVIFTIDASATSVCSIASGDVSF
                     HAIGTCVVNANQAGNSNYAAAAQVQQSFAVGKGTPIISWANPADITYGTPLSATQLNA
                     TASVLGTFTYSPVLNSVLGAGAGQILSVDFTPNDTVNYFSVNGTTVTINVLKADPILS
                     VTNSPVEYNGSPRVAVVASSVPGTISNILYDGSLNVPRNIGTYAITADFVPTDGVNYN
                     SLVAGSAGDFAIVPQGPDYPTFADVPMNHWAWAYIESIYAAGITGGCGTSPLIYCPSD
                     PVTRAQMAIFILRGVHGKAYVPPPASGTVFIDIPTDYWAADWIEQLAEEGITVGCGDG
                     NYCPENAITRAEMSVFLLRSKLGSTHTPPPATGTTFADVPANYWAASWIEQLALMEIT
                     SGCGDGNFCPEGQVTRDQMAVFIQRMFNFPLP"
     gene            1752..3245
                     /locus_tag="PL000_15710"
     CDS             1752..3245
                     /locus_tag="PL000_15710"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90381.1"
                     /translation="MQALTKKLPFWLKLLYGSGDWGLSSIGMMRSIFYALYLTDVVGI
                     EPRLASIGALAGIVWDAINDPLIGMISDRIQTRWGRRRPFLLWFAFPFGLSFVILWSA
                     PNWESQIALTIYVTLAFMISDTLTTLVAMPYLALTPELTRDYDERTTLSSFRTVFQLL
                     AAMMVVITAPMIVDQVIHNGGTQQQGFMLAGAVFGGLSAVPLFLIGWFVHEKFQPEPQ
                     EELPFRETLRIAWKNIPFRYAAGIYMFNWSAVDMIAIIFPYFLLYWVAEGNLLAKVNI
                     LGIDLALESAFFGIMMLVCILCVPFWLWLAKTRNKREAYILGMSFWVIVQTMIFTIQP
                     GETGYLLFIGALAGVGVSAAYILPDSILPDVIEWDELRTRRRQEGIYYGIRTLIRKLT
                     GAMVIFLTLQILGWSGYQAPPDGAIQSTQSASALIAIRLMVSLLGAVILAGTILLAWS
                     YPLSREKYERIQKLLKRRRNETPPPPKPTTDPVSTTDSPETQSAKPI"
     gene            complement(3315..5000)
                     /locus_tag="PL000_15715"
     CDS             complement(3315..5000)
                     /locus_tag="PL000_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC90382.1"
                     /translation="MYDRKKLDELKNSLETWEATSLKKALSQLPERKAENEFITTSSE
                     PINRVYTPQDVAELDHETDLGLPGEYPYTRGVHPTLHRSKLWTMRMFAGFGTAEETNK
                     RFKYLLEQGQTGLSIAFDLATLMGYDTDQPEALGEFGKCGVAISSLKDMEILLEGIPL
                     DKVSSSMTINSPAAIIWAMYIAAAENKGVRADQLRGTTQNDILKEFIAQKEFIFPPEP
                     SMRLVVDTMEFGAQKVPQWNTISISGYHIREAGSTAAQELAFTLADGLEYVRWGIARG
                     MDVDDFAPRLSFFFNAHNDFFEEIAKYRAARRIWAREMRETFKAKNPRSWLLRFHTQT
                     AGVSLTAQQPENNVVRVAIQALAAVLGGTQSLHTNSLDEALALPSEHAVTIALRTQQI
                     IAEESGVTNTIDPLGGSFFVEAQTDRMEKQVYDYFRRIEELGGVIPSIEKGFFQSEIA
                     DAAYRYQREIDLGVRKIVGVNAYAEDKPYTIPILKMDPNGYTRQVGRLNEVRKTRDSG
                     RVGQALDRLRIACQGTENTMPHIMECVHAYATLGEIVAVMKETFGVYEELSVI"
     gene            5132..5659
                     /locus_tag="PL000_15720"
     CDS             5132..5659
                     /locus_tag="PL000_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HNC90383.1"
                     /translation="MPRFEIDIDPCDHITADAIGAPGQRVFYLQAYQDQRTITILIEK
                     AQLQSLAIGIEQFLSQINQQNPNLEEARGEFSEDQMRIHPPVDPLFRAGEIGLGYDKE
                     RDRVVIFTKELLTEQDDPEAAAQIRFWASRTQMRKLARWGADVVSRGRPTCPQCGQPE
                     EPGGHFCPKKNGHLS"
     gene            5715..6425
                     /locus_tag="PL000_15725"
     CDS             5715..6425
                     /locus_tag="PL000_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HNC90384.1"
                     /translation="MLPHPHQEIQAALTHGDYELKGQFMLGSNYTFFVDVQHEGKIFK
                     AVYKPSKGEQPLWDFPDNTLALREVAAWRLSEALGFHIVPFTTYREDGPYGPGSLQQY
                     IEYNIEHHYFNFTPEERETLRPVVLFDILANNADRKGSHVFFEEGTHKLYGIDHGICF
                     HQENKLRTVIWDFSGQQIPDDLLAPLAEAENWPGLFEQYLSPGEIRALQRRARTLLKS
                     GLFPLRPVDRRAFPYPPI"
     gene            complement(6471..6602)
                     /locus_tag="PL000_15730"
     CDS             complement(6471..6602)
                     /locus_tag="PL000_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90385.1"
                     /translation="MNTGLFIIVAAAFVLYGLTRTTESILDVRQAERDNGEDVKIYL"
     gene            6868..7902
                     /locus_tag="PL000_15735"
     CDS             6868..7902
                     /locus_tag="PL000_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC90386.1"
                     /translation="MKHYKLALIGFGNVARALARLLVRKQSLLEQQGITFSFTGISTG
                     RHGFAVNPDGLDIQKALELTESGQSISSLSTTPVADSLAVIKNSSANVMFENSPVNTE
                     TGQPALDHMLTALNLGMHAITANKGPVVHGFSELNALADKMGKKFRYESTILGGSPVF
                     SVLREAFPLAEVSSFKGIFNATTNLILSRMENGETYEDAVKYAQSIGVAEADPTNDVD
                     GWDAAFKVSALVRVLWNTPMTPQQVKPTGIRGITSEMIAKAKAEGKRYKLVCFGEKDG
                     DAVKVSVSPQLVDATSPLYGMMNSSTGVTFRTDVILDYSIILSEKPGMQGGPVETAYG
                     LFADFVNIVK"
     gene            complement(7976..8824)
                     /locus_tag="PL000_15740"
     CDS             complement(7976..8824)
                     /locus_tag="PL000_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90387.1"
                     /translation="MPITRDSVKTYYDQNTRLFLAFGKNNKRKAQNIHRSLWVEGVQT
                     LEDALNVSNANILKEIEAIAPQQARVADLGCGVGASLFYIYPRLQMPNHALGLTLSPV
                     QARLARGTATQLGLEGQILFAEGDFTSVPLGSGLDAVYSVEAVCHAVDPERYFREAAR
                     LLRKGGRLMLVDDYQAARNFSQAESMWLKAYMDGWYVPGVRTVEQATEWAEQYGLRLI
                     RNDELTPHLRLRNLPDLLARLILFLGNHLPIKHAILPSMLGSMALQQCLHMGIVEYRF
                     LVFEKA"
     gene            complement(8844..9851)
                     /locus_tag="PL000_15745"
     CDS             complement(8844..9851)
                     /locus_tag="PL000_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNC90388.1"
                     /translation="MLRRFLHLTLLALILSACGVTALPSQPILAQPPTVTLIPSITPP
                     PPTATAIPTSTATPVPKSRRVLIVSFDGLRPDAIAAANMVNVMSLMKSGAYTLTAQTI
                     MPTLTLPAHSSMLVGTCPAKHIVRWNEYVPENGFAQGVDLFDIAHAAGLRTVMVVGKE
                     KLRQVTEETSTDYFAFVDKTDKIEDEFAIVQLAIQQLSEGFDLMFVHFPNGDLEGHDS
                     GWMSVAQLRTYANDDESLGFILQSLEKRDMLKDTLIIITSDHGGHDSTHGTDLPEDMT
                     IPWIVSGPGVIPMPLTTQVHTMDTAATAAFSLGLSIPPEWDGVPIYEAFGMPIVPSEG
                     C"
     gene            complement(9854..11515)
                     /gene="hutU"
                     /locus_tag="PL000_15750"
     CDS             complement(9854..11515)
                     /gene="hutU"
                     /locus_tag="PL000_15750"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNC90389.1"
                     /translation="MSTPRVIRSPHGTQLTCKNWLSEAVYRMIQNNLDPEVAEKPEDL
                     VVYGGRGKAARNWEAFDAILESLKNLEADETLLVQSGKPVVVFKSHKDAPKVLIANSN
                     IVPHWATWEKFDELDRKGLIMYGQMTAGSWIYIGTQGILQGTYETFGALAKLKGWGSL
                     KGKFVLTAGLGGMGGAQPLSITMNEGVGLIVEVDPERAERRRAIGYVDMVVDNLEEAM
                     TLVEENVKNGTPKSIGLIGNAADVYSELAARGIIPDVVTDQTSAHEALFYVPSGISLT
                     AANQLRVSDPEKYKKMAMDSMAKHVQAMLAFQRAGAEVFDYGNNLRQQAYNHGVTDAF
                     EFPGFVPAYIRPLFCEGKGPFRWVALSGDKEDIYTTDRAIMELFPEDEHLHRWLKMAK
                     EKVPFQGLPSRICWLGYGERAKAGLMFNELVASGKVKAPIVIGRDHLDSGSVASPNRE
                     TEAMKDGSDAISDWAILNAMINAVGGATWVSFHHGGGVGMGYSQHAGQVIVADGTPEA
                     AQRLERVLTTDPGMGVVRHADAGYEIAIDAAKRHGIKMPMLGKSS"
     assembly_gap    11539..11638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11695..12543)
                     /locus_tag="PL000_15755"
     CDS             complement(11695..12543)
                     /locus_tag="PL000_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90390.1"
                     /translation="MTHRKTSFFALILLVTVTACVIPGLSTPTAPPLFAPTTDPGRLE
                     TMVAETVVAAIAETEQSQPTPTRIPTSQPTSTATPEPITAGSTLTLQEDGTTTFLDER
                     AGYQITIPDGWLAVRVDEQEYYDAWNLAEATDPNIQAILLGMKEQDPNVQRLRAIDTQ
                     DGHIQSDFVTDISFIWNEQGKFETIEDLQAIASELPNAPSAFRFEVATVQTIIAPSGI
                     QFGVIEATSSFTNTSGDLVNVYLKQIVFKAKTGTQSIILLTVEGLKETTLPAFDGMIE
                     TIKLNQ"
     gene            complement(12620..13033)
                     /locus_tag="PL000_15760"
     CDS             complement(12620..13033)
                     /locus_tag="PL000_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC90391.1"
                     /translation="MQITRQADYAVRAVLHLARTGDQRTATSTIAEEQHIPPSFLAKI
                     ISQLSIAGLLHTSRGARGGVTLAREAKEITLLEVIEAIDGPIQLNECVGENGICSFDE
                     SCPLRPVWCDAQEELVGRLKGTNFADMIAKGQTSV"
     gene            complement(13231..14079)
                     /locus_tag="PL000_15765"
     CDS             complement(13231..14079)
                     /locus_tag="PL000_15765"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HNC90392.1"
                     /translation="MLRKLILLIAILALASMACGFNINIPDAPKPGPQVTDDINVKAP
                     KADDINLKLSFGAGEMILAPGADQLVAGTASYNYSAFKPNINTDGGNVSITMSEVDFK
                     TFPNFNEFKNKWDFELGDNPMNLSIESGAYDGTFELGGLALENLSIKDGAANVVLSFS
                     EPNLSEMSTFVYETGASDVKMTGLANANFSLMDFSSGAGDYTLDFSGELQRDASIKIS
                     SGLSNIIIVVPEGVNAVVTMDGGASNISVGSSWSMNGGTYMQKGEGPTLTFVIEIGAG
                     NVTLTH"
     gene            complement(14149..15567)
                     /gene="radA"
                     /locus_tag="PL000_15770"
     CDS             complement(14149..15567)
                     /gene="radA"
                     /locus_tag="PL000_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC90393.1"
                     /translation="MAKTHTRYVCQQCGRVAASYMGKCPQCGSFSSMVEEVIHDEPVS
                     KGSSNARGLSGRSEPRPISEVGGEAEDRVALPITEFSRVLGGGIVLGSIVLIGGDPGI
                     GKSTLMLQMAMEMAGIQKVLYVSGEESERQVKMRAVRLLGGKKELPKNLLLVTETNLE
                     VILNHVNELKPDLLIVDSIQTVYLSELDSSAGSVSQVRECASQLRELAKSSGVSVFMI
                     GHVTKEGAIAGPRVLEHIVDTVLYLEGDRFQAYRLLRSVKNRFGATAEVGVFEMREGG
                     LVEVTNPSEAFLAERLVNAAGSAIAVTMEGTRPILVEIQGLTSPTQFGNARRTPNGVD
                     FNRLLLISAVLTRRVGLKLSEQDVFVNVVGGIQIDEPAADLAVAAAIASSWKDVPVRA
                     EAVLIGEIGLAGELRMPGQMVARLREAQKLGFKAAIVPKAIRQGEAYPKGIEIVEVRS
                     LDQALNTALMKDGLASKARKSE"
     gene            15725..15798
                     /locus_tag="PL000_15775"
     tRNA            15725..15798
                     /locus_tag="PL000_15775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15759..15761,aa:Arg,seq:gcg)
BASE COUNT         3833 a         4391 c         3820 g         3773 t
ORIGIN      
        1 tcaacgatgc caattatgct ggctctgcca atggcaccct cgtcatctcg aaggctaatc
       61 agaacatctc attcactaca gctgccccgg ttggtgcttt ggttggtggt ccggtctata
      121 ctccggcggc ttcctccacc tcaagccttc ttgtgtccat caccattgat ccatcctcag
      181 ctgcggtatg ttccatttcc ggcggcgaca tttccttcct tagcacaggc acgtgtaaag
      241 tcaatgccaa tcaagctggg gattcgaatt acaaccccgc ttcacaggtc caacagtcct
      301 tcggtattgg ccttggaaac caggttattt cgatgacctc gtctgttcca gccgcagccg
      361 tggttgacgg acctgtttac acaccaagtg caacaggcgg cgcttctggt aacccggtta
      421 tctttaccat tgatgcctcc gcaacttcgg tctgctccat tgccagtggc gatgtttcct
      481 tccatgcaat tggtacttgt gtggttaatg caaaccaagc tggtaattcc aactatgctg
      541 cagctgcaca agttcaacaa tcctttgctg tgggcaaggg cacgcctatt atttcatggg
      601 ccaatcctgc cgatattaca tatggcaccc cgctgagcgc cacacaactt aatgccactg
      661 cttcagtact tggcacattt acatacagtc ctgtactgaa ttctgttctg ggagcaggtg
      721 caggccagat actgtccgtt gacttcacgc caaacgacac agtaaattac ttcagtgtca
      781 acggaaccac tgttaccatc aatgtcctca aggcggatcc catcctgtct gtcacaaata
      841 gcccggttga atataacgga tctccgcggg tagccgttgt agccagttca gtacctggca
      901 cgatcagcaa cattctctat gatggctcct taaatgttcc cagaaacatc ggtacctatg
      961 ccattactgc tgattttgtt ccgaccgacg gagtcaacta caacagcctg gtcgccggat
     1021 cagcaggtga ctttgcaatc gtgccacaag gtccagacta tcccaccttt gcagatgttc
     1081 ccatgaatca ctgggcctgg gcgtatattg aatcgatcta tgctgcggga attacgggtg
     1141 gctgcggaac gtcaccgctg atctactgcc caagcgatcc cgtcacacgt gcgcagatgg
     1201 caatctttat tctgcgtgga gttcatggca aggcatatgt cccgccgcca gccagcggaa
     1261 ccgtgttcat tgatattccc accgattact gggccgcaga ctggatcgag caactcgctg
     1321 aagaaggaat cacggtcggt tgtggtgatg gaaattactg tccagaaaat gccattaccc
     1381 gggctgaaat gtcggtcttc ctgctgcgct caaaactggg cagcacccac acaccgccgc
     1441 ctgccaccgg caccaccttc gcagatgtcc ccgccaatta ctgggcagcc agctggatcg
     1501 aacagcttgc cttaatggag atcaccagcg gttgtggtga cggaaacttc tgtccggaag
     1561 ggcaggtcac ccgcgaccag atggcagtct tcatccaacg catgttcaac ttcccgctgc
     1621 cgtaaccgcc tttccccact atcattaaat aaaccagccg caggtaatct gcggctggtt
     1681 tatttaaact ttatgctaca ctatccccat ccacgcatct caaaatccca ctcggtgttt
     1741 attggtacat tatgcaagcc ctgacaaaaa aacttccctt ctggcttaaa ctcttatacg
     1801 gctccggcga ctggggcctt tccagcatcg gtatgatgcg ctccattttc tacgcactct
     1861 acctcactga tgtggttggg attgagccgc gtctcgcctc cattggcgca ttggcgggca
     1921 tcgtctggga tgcgatcaac gaccctctga tcggcatgat cagcgaccgc attcaaacac
     1981 gctggggcag aaggcgcccc ttcctattgt ggtttgcctt tcccttcggc ttaagtttcg
     2041 tcatcttatg gtcagccccc aactgggaaa gccagatcgc tttaacgatc tacgtcacgc
     2101 tcgccttcat gatctccgac acgctcacca cgctcgtagc catgccctac ctggctctca
     2161 caccggaact cacccgcgat tacgacgaac gcaccaccct ctccagcttc cgaactgttt
     2221 ttcaattgct tgccgcaatg atggtcgtca tcaccgctcc gatgatcgtt gaccaggtca
     2281 ttcacaacgg cggcacacaa cagcaaggct tcatgcttgc aggcgccgtt ttcggcgggt
     2341 taagcgccgt tccgcttttt cttatcggct ggttcgtgca tgagaaattc cagccagagc
     2401 cgcaggaaga actccccttt cgtgaaaccc tccgcattgc atggaagaac atccccttcc
     2461 gttacgctgc aggcatttac atgttcaact ggtcggcagt ggatatgatc gccatcatct
     2521 tcccctactt tttgctctac tgggtcgccg aaggcaacct gcttgccaag gtcaacatcc
     2581 tcggcataga tctcgcactt gaatccgcct tctttggcat catgatgctc gtatgcatcc
     2641 tctgcgtccc cttttggcta tggctcgcaa aaacccgcaa caaacgcgaa gcctatatcc
     2701 tcggcatgtc cttctgggtc atcgtgcaaa ccatgatctt caccatccaa ccgggcgaaa
     2761 caggttacct gcttttcatc ggcgccctgg ccggggtcgg tgtttcagcc gcctacatat
     2821 tacccgactc gatcctgccc gacgtgatcg aatgggacga actgcgcaca cgccgcagac
     2881 aggaaggcat ctactacggc attcgcaccc tcatccgcaa gttgaccggc gcaatggtca
     2941 tcttcctcac cctgcaaata ctcggctggt ccggatatca agccccgcca gatggggcca
     3001 tccaatccac gcaatctgct tctgccctga tcgcaattcg attaatggtc tccctgctcg
     3061 gcgccgtcat ccttgcaggc acgatcctgc tcgcatggtc atatccgctc tcacgcgaaa
     3121 aatacgagcg gattcaaaaa ctcttaaagc ggcgcaggaa tgaaaccccg cctccaccca
     3181 aaccgacaac tgatcctgtt tcaacaacag attctcccga aacgcaaagc gccaaaccca
     3241 tttaacgata cccacccaaa caaaaaacgg acagcttttg ctgtccgttt tttgttttca
     3301 ttgaatcagt ctatttaaat cactgacaac tcttcataca caccgaaggt ttccttcatc
     3361 actgccacga tctcacccag ggtggcatac gcatggacac attccatgat atgaggcatt
     3421 gtattctcag taccctgaca tgcaattcgc agccgatcca aagcctgccc cacgcgtccg
     3481 ctatcacgcg tcttacgaac ctcattcaag cgccccacct gacgggtata accatttgga
     3541 tccatcttaa gaatgggaat ggtatatggc ttatcctcag cataggcatt cacaccgacg
     3601 atcttgcgca ctcccagatc gatctcacgc tgatagcgat acgctgcatc tgcgatttcg
     3661 ctctggaaga agcccttttc aatggatggg atcaccccgc ccaattcctc gatcctacgg
     3721 aaataatcat aaacctgttt ttccattcgg tcggtctgcg cttctacaaa gaaactaccg
     3781 cccaatggat cgatggtatt tgtcacgccc gattcttcgg caatgatctg ctgggtacgc
     3841 aaggcgatcg tcaccgcatg ttccgaagga agcgccaagg cctcatccaa tgaattggta
     3901 tgcaggcttt gtgtgccgcc caacactgcc gccaaagcct gaattgccac acgaacgaca
     3961 ttattctctg gctgttgtgc cgtcaacgaa actccggccg tctgtgtatg gaaacgcagc
     4021 aaccacgaac gcggattctt cgccttgaag gtctcacgca tctcacgtgc ccaaatccgt
     4081 cgtgccgcac gatacttggc gatctcttca aagaaatcgt tgtgcgcgtt aaagaagaac
     4141 gacaaccgcg gcgcaaaatc gtccacatcc ataccacggg caatgcccca acgcacatat
     4201 tcgagaccat cagccagggt aaaggccagt tcctgtgcag cggttgaacc cgcctcacgg
     4261 atatggtacc cgctgatcga aatcgtattc cactgcggca ccttttgagc gccgaactcc
     4321 atcgtatcca ccaccagcct catcgaaggc tcgggcggaa agataaattc cttttgtgca
     4381 atgaattcct tgaggatgtc gttttgcgtg gtgccgcgca actgatcggc gcgcactccc
     4441 ttgttctcag cagcagcgat atacatcgcc caaatgatcg cagcaggtga attgatggtc
     4501 atgctcgaag agaccttatc caacggaatg ccttcgagca aaatttccat atccttcaag
     4561 gatgagatcg ccacgccgca ttttccaaat tcacccaacg cctcaggctg gtcggtatca
     4621 tagcccatta aagttgccag gtcgaaggca atgctcaatc ctgtttgacc ctgttccagt
     4681 aagtatttga agcgtttgtt cgtttcctcc gcagtgccga accctgcaaa cattcgcatg
     4741 gtccacaatt tgctgcggtg cagagtcggg tgaaccccgc gcgtgtaggg gtattcaccc
     4801 ggcaatccaa gatcggtttc atgatccaat tcagccacat cctgaggcgt atagaccctg
     4861 ttgatgggtt ctgaagaggt ggtgataaat tcgttttcag ccttgcgctc agggagctga
     4921 gacaacgcct tcttcaggga agtcgcctcc catgtttcaa gcgagttttt caattcatcc
     4981 aatttttttc tatcgtacat ggcacactcc tttggcggga tgtacggatt atacatgagg
     5041 cgtagtctgg tatactacca aaaatacact tttaaaaaaa acagacaaac aggtggtttc
     5101 atctgtccaa tgattttaca aaaggtaaca tatgcctcgt ttcgaaattg acatagatcc
     5161 atgtgatcac atcactgccg atgccatcgg cgccccgggc caacgcgtct tttatctgca
     5221 agcctaccag gaccaacgca cgatcaccat cctgattgaa aaagcccagt tgcaatcgct
     5281 cgccatcgga atcgaacaat tcctctcaca gatcaaccag caaaacccca acctcgaaga
     5341 ggcgcggggt gaattctctg aagatcaaat gcgcatccac ccaccggtag acccgctctt
     5401 ccgtgccggt gagatcgggc ttggctacga taaagaacgc gaccgcgtcg tgatcttcac
     5461 aaaagaattg ctcaccgaac aggatgaccc cgaagccgca gcacagatcc gcttttgggc
     5521 cagccgcacc caaatgcgca aactcgcccg ctggggcgca gatgtggtca gccgtggacg
     5581 ccccacctgc ccgcaatgcg gccaacctga agagcccggc gggcatttct gcccaaagaa
     5641 aaacgggcac ttgagttaag ttgaacgttg ctggttgaaa gtttcaacca gcaacgtgta
     5701 accttcagcc atttatgctc ccacatcccc atcaagaaat tcaagccgcc ctcacccacg
     5761 gtgattacga actcaaaggc cagttcatgc tgggctcaaa ttacacattc tttgtggatg
     5821 tgcagcatga aggcaaaata ttcaaagctg tgtacaaacc cagcaaaggc gagcagccgc
     5881 tttgggattt ccccgataat acgctggccc tgcgtgaagt agccgcctgg cgactaagcg
     5941 aagcgctcgg ctttcacatc gttccgttca ccacctatcg cgaagatggc ccatacggcc
     6001 ccggttccct gcaacaatac atcgaataca acatcgaaca tcattacttc aacttcaccc
     6061 ccgaagagcg tgaaaccctg cggcccgttg tgctattcga tattctcgcc aacaatgccg
     6121 accgcaaagg cagccatgtt ttttttgaag aaggcacaca caaactatac gggatcgatc
     6181 atggaatttg ctttcaccag gaaaacaaac tacgcaccgt aatctgggat ttctccgggc
     6241 aacagattcc agacgacttg cttgcgcctc tcgccgaagc cgaaaactgg cctggcctct
     6301 ttgagcagta tctcagcccg ggcgagatcc gcgcactcca acgccgcgca cggacattgc
     6361 taaagtcagg gttgttcccg ttacgacccg ttgatcgaag agcgtttccg tatcccccga
     6421 tctaatcaaa aagggacact gaaaattcag tgtccctttt tttcgtccat ttacaaataa
     6481 atctttacgt cttcgccgtt atctctttcg gcttgacgaa catcaagaat ggactcggtc
     6541 gtgcgggtaa gcccgtataa aacgaaggct gctgcaacaa tgatgaacaa tccggtattc
     6601 attttgattc tcctttctat ttccatgcac aaacacccgg aagaacagac gcaaaactaa
     6661 agatttgcag acaaaatagg tcaacaaatt tatgataaaa agacttccgc taagcttcgt
     6721 gaagggatgc acaagtatta taacccaatt cagcattccc ataccagaaa aaacccttgg
     6781 cggataaaat aacgcccaca aaaatgattt gaaacccgaa cacggctctt tcaacaattc
     6841 acgaaataaa cgaaaacgga gacctcaatg aaacactaca aactcgcact gatcggcttc
     6901 ggcaacgtag cccgtgccct tgcccgattg ctcgtccgca aacaaagctt gcttgaacaa
     6961 cagggaatca ccttctcatt cacgggcatt tccaccggca ggcacggatt cgccgtcaac
     7021 cccgatggcc tcgacattca aaaggcgctg gaactcaccg aaagcggaca atccatttcg
     7081 tcgctttcca ccactcctgt ggccgattcg ctggcggtca tcaagaactc atctgcaaat
     7141 gtcatgttcg aaaactcacc cgtcaacact gaaaccggcc aacccgccct ggaccatatg
     7201 ctgactgctt tgaacctggg aatgcacgcc atcaccgcaa acaagggacc tgttgtacat
     7261 ggtttcagcg aattgaacgc cctggcagat aagatgggca aaaagttccg atacgaatcc
     7321 accattttgg gtgggtcacc cgtcttctcg gtcctgcgtg aagcgttccc tctggcagaa
     7381 gtctcctcct tcaagggcat cttcaacgcc acaaccaatc tcatcctctc acgcatggaa
     7441 aatggtgaga cctacgaaga tgccgtcaaa tatgcccagt ccattggtgt ggcagaagct
     7501 gacccaacca atgatgtgga tggctgggat gcagccttta aagtttccgc gctggtacgg
     7561 gtgttgtgga acacgccgat gaccccgcaa caggtcaaac cgactggcat tcgcggaatc
     7621 acaagcgaaa tgatcgcaaa agccaaagct gaagggaaac gctataaact ggtttgcttc
     7681 ggtgaaaaag acggagatgc ggtcaaggtc agtgtttctc cccaattggt ggatgcaact
     7741 tccccgctgt acggaatgat gaactccagc accggcgtca ccttccgcac cgatgtaatt
     7801 ctggattact cgatcatcct ctctgaaaaa cccggtatgc aaggcggacc tgtggaaacc
     7861 gcctatggtt tgtttgcaga ctttgtgaat atcgttaagt aaaaagggtg cgacgcactc
     7921 aaaagaaaaa acggcgatga aaataaatca tcgccgtttt tcttgtttat tctcattatg
     7981 ctttctcaaa caccaaaaac cgatactcaa cgatccccat gtgcaggcat tgctgcagcg
     8041 ccatgctccc aagcatggat ggaagaatgg catgtttgat gggcaagtga ttgccaagga
     8101 agaggatcaa acgagccagc aggtcgggca gattacgcag gcggagatgt ggagtgagtt
     8161 catcatttcg gatcaggcgc aggccatatt gttcagccca ctcggtcgcc tgctccaccg
     8221 tccgcacacc ggggacatac cagccatcca tgtatgcctt caaccacatt gattcggctt
     8281 gtgaaaaatt ccgcgccgcc tgatagtcat ccaccagcat caacctgccg cctttccgca
     8341 acagacgggc cgcctcacgg aaatatcgct cgggatctac ggcatggcaa accgcttcca
     8401 cagaataaac cgcatccaaa ccgctcccca acggcacaga cgtgaaatca ccttctgcaa
     8461 aaagaatctg accctccagg cccaactggg ttgctgtacc gcgcgccagc cgggcctgca
     8521 ccggactcaa tgtcagcccc agtgcatggt tcggcatttg caaacgcgga taaatataaa
     8581 aaagacttgc acccaccccg cagcccaggt ctgcaacgcg ggcctgttgc ggagcaatgg
     8641 cttcgatctc ttttaagata ttcgcattgg aaacattaag tgcatcttca agagtctgca
     8701 caccctccac ccacaaagag cggtgaatat tctgtgcctt gcgcttgttg ttttttccaa
     8761 acgcaaggaa cagacgggtg ttttggtcgt aataggtttt aacggaatca cgtgtaattg
     8821 gcatattttt ttctgtatcg ttatcaacaa ccttcagatg gcacgatcgg cataccgaag
     8881 gcctcataga tcggcactcc gtcccattcg ggcggaatgg aaagccccag tgaaaatgca
     8941 gccgtcgcgg ccgtatccat cgtatggact tgcgtggtga gcggcatggg gatcacacct
     9001 ggcccgctta cgatccacgg aatggtcata tcttcgggca ggtcggtgcc atgagtagag
     9061 tcatgaccgc catgatcgga ggtaatgatg atcagggtat ctttgagcat gtcgcgtttt
     9121 tcaagcgatt gcaggatgaa ccccagcgat tcgtcatcat tggcataagt acgcagctgc
     9181 gcaaccgaca tccaacccga atcatgtccc tccagatcac catttggaaa atgcacgaac
     9241 atcaaatcga agccttcgct gagttgttga atggcaagct gcacgatcgc aaactcatcc
     9301 tcgatcttgt cggtcttgtc tacaaaggca aagtaatcgg tgctggtctc ttcggtaacc
     9361 tggcggagct tctccttgcc aaccaccatc accgtccgca agccagcggc atgggcaata
     9421 tcaaaaaggt caacaccctg cgcaaatcca ttttcgggca cgtactcatt ccaacgcaca
     9481 atatgtttgg cggggcaggt gcccaccaac atggatgaat gtgcaggcag tgtgagggtt
     9541 ggcatgatgg tttgtgcggt caatgtgtag gcgccacttt tcatcaacga catcacgttc
     9601 accatgttgg cagcggcaat ggcatcgggc cgcaggccgt cgaaacttac gatcaagaca
     9661 cggcggcttt tcggcacagg ggtggcggtg ctggtgggaa tcgccgttgc cgttggaggc
     9721 ggcggggtga tgctcgggat gagagtgact gtcggaggct gggcaagaat cggctgggaa
     9781 ggaagcgcag tgactccaca tgcggagagg atcagtgcaa ggagagtcaa atgcaaaaaa
     9841 cgccgaagca tgttcaggat gatttcccaa gcatcggcat ttttatcccg tgacgttttg
     9901 cggcgtcaat ggcaatttca taacccgcat cagcgtgtct gaccacgccc atgcctggat
     9961 ctgttgtaag gacacgttcc aacctttgag cggcttcggg cgtgccatcg gccacgatga
    10021 cttgcccagc atgttggcta tagcccatgc ccacaccgcc gccgtgatgg aacgaaaccc
    10081 acgttgcgcc gccgactgca ttgatcatgg cgttgagaat cgcccaatcg gagatggcgt
    10141 cggaaccgtc tttcatggct tcggtttcgc ggttgggaga tgccaccgaa ccagagtcaa
    10201 gatgatcgcg gccaatgacg atgggcgctt tgacttttcc gcttgcaacg agttcgttga
    10261 acatcaggcc tgccttggca cgttcgccat aaccaagcca gcaaatgcgc gagggcaatc
    10321 cctggaaggg gactttttct tttgccatct tcagccagcg gtgcaggtgt tcatcttcgg
    10381 ggaagagttc catgatggcg cggtcggtgg tgtaaatatc ttccttgtcg ccggagagcg
    10441 ccacccagcg gaaggggcct ttgccttcgc aaaacagcgg acggatgtag gcgggcacaa
    10501 agccggggaa ttcgaaggca tctgtcacgc cgtggttgta ggcttgctga cgcaggttat
    10561 tgccataatc gaacacttcc gcgcctgcgc gttggaaggc aagcatggct tggacgtgct
    10621 tggccatgga atccattgcc atctttttat atttttcggg gtcactcacg cgcagttgat
    10681 ttgcggcggt gaggctgatt ccagatggga cataaaataa cgcttcgtgc gcagaggtct
    10741 ggtcggtgac cacatcgggg atgatcccgc gcgcggcaag ttcggaatat acatcggcgg
    10801 cgttgccgat caggccaatg gatttgggcg ttccgttctt tacattttct tccacgagcg
    10861 tcatggcttc ttcgaggtta tccacaacca tatccacata gccaatggcg cggcggcgtt
    10921 cggcgcgttc ggggtccact tcaacgatca ggccgacacc ctcgttcatg gtgatcgaaa
    10981 gcggttgggc gccgcccatg ccgccaagtc ccgccgtcaa aacaaattta cctttgagag
    11041 atccccaccc cttaagtttg gcgagtgcgc caaaggtttc gtacgtgccc tgcaaaattc
    11101 cctgtgtgcc gatgtagatc catgaaccgg cggtcatctg cccgtacatg atcagtccct
    11161 ttctatcgag ctcgtcaaat ttttcccagg tggcccaatg cggcacgatg ttggagttgg
    11221 cgatcagaac tttgggcgca tccttgtgcg atttgaaaac gaccaccggc ttgcccgatt
    11281 gcacaagcag ggtttcatct gcttcgagat ttttcaaaga ttcaaggatt gcatcgaagg
    11341 cttcccagtt gcgggcggct tttccgcgcc cgccgtaaac gacgaggtct tcgggctttt
    11401 cggccacttc gggatcgagg ttattctgaa tcatgcgata gacagcttcg ctgagccagt
    11461 tcttgcaggt tagctgtgtt ccatgcggtg agcggatgac gcggggtgtg gacattgggg
    11521 aatctccttg gtgaaattnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg
    11641 cggtgagcgg atgacgcggg gtgtggacat tggggaatct ccttggtgaa atttttactg
    11701 atttaattta atcgtttcga tcatcccgtc aaatgcgggc agggtggttt ctttcaaacc
    11761 ttccacagta agcaggataa tggattgtgt tcccgttttg gctttgaaaa cgatctgctt
    11821 caagtacaca tttaccagat caccggaagt gtttgtgaac gaagaagtgg cttcgatcac
    11881 accgaattgt atcccgctcg gagcgatgat ggtttggaca gtggcgactt cgaacctgaa
    11941 agcagagggg gcattgggca gttcactggc aatggcttgc aggtcttcga tggtttcgaa
    12001 tttcccctgt tcgttccaga taaaactgat atcggtcaca aaatcgcttt ggatgtggcc
    12061 gtcctgcgtg tcaatggcac gcagacgctg aacatttggg tcctgttctt tcatgcccag
    12121 aaggatcgcc tggatgtttg ggtctgtggc ttcggctaaa ttccaggcat cgtagtactc
    12181 ctgctcgtcc acgcgtacgg cgagccagcc atcagggatg gtgatctggt acccggcacg
    12241 ttcatcgagg aaggtggttg tgccgtcttc ctgtaaggtg agcgtactcc ctgctgtgat
    12301 gggctccggg gtggcagtgg aagtaggttg ggaggtcggg atgcgtgtgg gggttggttg
    12361 tgattgttca gtttcagcga tggcggcgac aactgtttca gcgaccatgg tttccaggcg
    12421 gccgggatcc gttgtcggcg cgaagagagg cggggccgtc ggggtggaaa gtcccggtat
    12481 gacacaggct gtgactgtta ccagaaggat cagtgcgaag aaagatgtct ttctatgggt
    12541 catggattcg ttccttttcg caaaaataaa aaagcctccc ggctattata gccgagaggc
    12601 ttcaggatgc tttcgcgctt tagacggaag tctgtccttt tgcgatcata tccgcaaaat
    12661 tggtgccttt caacctgccc accagttcct cttgtgcatc acaccatact gggcgcaagg
    12721 ggcatgattc gtcgaaggag caaataccat tttcgccaac acattcatta agttgaatgg
    12781 ggccgtcaat ggcttcaatg acctccaaaa gggtgatctc ttttgcttca cgggcgagtg
    12841 ttacgccgcc gcgcgcaccg cgagatgtgt gcaacaaccc tgcaatggat aattgggaga
    12901 tgatcttggc caaaaaggat ggcggaatat gctgttcttc agcaatggtg ctggtggctg
    12961 tgcgctgatc gccagtgcgg gcaagatgga gcacggctcg aacggcataa tcagcctgac
    13021 gtgtaatttg catgataacc tctcttaaaa caggtaaact ttaccgataa tcacgatttt
    13081 attgtgtaag gtagtacaag gcaagtgatg taggtcatag ataaattatg accttttgct
    13141 ccgaaatggg gtggaaaccc catacaggtt taaacgaaat aaaaatgccc gcgtcaaagg
    13201 tttgatgcgg gcatttttgc gcaatagctt ttagtgtgtc aaagtgacat ttcctgcgcc
    13261 gatctcaatt acaaaggtaa gagtggggcc ttcacctttt tgcatgtaag taccaccatt
    13321 catgctccaa ctcgaaccca cgctgatatt cgaggcaccg ccatccattg taaccacggc
    13381 attcacacct tcaggaacaa caatgatgat gttgctcaat ccgctggaaa ttttgatgga
    13441 ggcatcacgc tgcaattcac cagagaaatc gagagtgtag tcacccgcac cggatgagaa
    13501 atccatcagg ctgaaattgg cattggcaag gcctgtcatt tttacatcgg aagcacctgt
    13561 ttcatacaca aaggtggaca tctcggacag gttgggctct gaaaacgaaa gcactacatt
    13621 ggcagcgcca tctttaatgg aaagattttc gagggcaagc ccgcccaact caaatgtacc
    13681 gtcataggcg cccgattcga tgctcaagtt cattgggttg tcgcccagtt caaaatccca
    13741 tttgttcttg aattcgttga aattcgggaa agttttaaaa tccacttcgc tcattgtgat
    13801 tgaaacattc ccgccatcgg tattgatatt gggtttgaat gcagagtaat tgtagctggc
    13861 agtacctgcc accagttgat ctgcgcccgg cgcaagaatc atttcaccgg cgccaaaaga
    13921 aagctttaaa ttaatatcat ctgccttggg ggccttgacg ttaatatcat cggtcacttg
    13981 cggaccgggc ttgggagcat ccgggatgtt aatattaaat ccgcaggcca tgctggccaa
    14041 tgcgaggatc gcgatcaata agattaattt tcgtaacatg gaaaatacct cctcaggtaa
    14101 tacagtgtct gaggaggtat acggctgatc ttttatttgg ttgcagggtt attcggattt
    14161 tctggccttc gatgcaagac cgtctttcat caacgccgta ttcaaggctt ggtccagcga
    14221 gcgcacttca acaatttcga tccccttcgg ataagcctca ccttgacgaa tggctttggg
    14281 aacaatggcg gctttgaatc ccaatttctg agcctcgcgc agtcgtgcca ccatttgacc
    14341 gggcatgcgt aattcaccag ccaggccaat ctcaccgatc aatacggctt cggctcgcac
    14401 aggtacatct ttccatgaag aagcaatggc tgcggcaaca gcaagatcgg cggcaggctc
    14461 atcaatctgt atgccgccca ccacattcac aaaaacatcc tgctctgaaa gtttcaaacc
    14521 tacacggcgg gtcaatacag ccgatatcaa cagcagacga ttaaagtcca cgccgttggg
    14581 ggtgcggcgt gcatttccaa attgggttgg ggaggtaagt ccctgaattt caaccagaat
    14641 gggacgtgtg ccttccatgg tcacagcaat ggccgagcca gctgcattga ccagtcgctc
    14701 tgcaagaaat gcctcagatg gatttgtcac ttcaaccaat ccgccttcgc gcatttcgaa
    14761 aacccccact tccgcagtcg cgccaaagcg gttctttacc gagcgcagca accgataagc
    14821 ctgaaagcga tccccttcaa gatacaacac ggtatccaca atatgttcca acacacgcgg
    14881 accggcgatc gcgccttcct tcgtcacatg cccgatcata aacacagata cacccgaaga
    14941 cttcgccaat tcacgcaact gcgaagcaca ctcacgcacc tgcgaaaccg accctgccga
    15001 cgaatccaat tcagacaagt acacagtctg gatcgagtca acgatcaaca ggtcgggctt
    15061 taattcgttg acatgattca agatcacttc aagattggtc tcagtcacca ataataagtt
    15121 ttttggcaat tccttttttc cacccagcaa gcgcacagcg cgcattttta cctgccgctc
    15181 cgattcctca ccggaaacat acagcacctt ctgaatcccg gccatctcca ttgccatttg
    15241 cagcatcaaa gtggatttgc caatgcccgg gtcaccccca atgaggacaa tggaaccgag
    15301 cacaattccg ccgccaagga cacgtgaaaa ctccgtgatc ggaagcgcca ctcgatcttc
    15361 cgcttctcct ccgacctctg aaatgggtcg cggctccgaa cggccactca gcccacgggc
    15421 attggatgat cccttggaca ccggctcatc gtggatcact tcctccacca tgctgctaaa
    15481 ggacccgcac tgtggacatt tgcccatgta agaagcggct acacgtccgc attgttgaca
    15541 aacatatcgg gtatgtgttt ttgccatatt cgatctccca taaggtgagg tagggcgagt
    15601 ataacagaat ttatgttctg ttttcaaagc ccgcccattt cacaaccacc gcacaccgac
    15661 ttaaaaacca aaaacgcttg accatcgtgc cacattacag taaaatacca cccgcataaa
    15721 aaacgcttcc gtagctcagt ggattagagc gcttgcttgc ggagcaatag gtcgcaggtt
    15781 cgagtcctgc cgggagcaca aaaaaccgtc taaatacagg cggttttttt atttaggtcg
    15841 cacgccccat cgagggataa tattcgagtc ctgccgggag cacaaaaaac cgtctaaata
    15901 caggcggttt ttttatt
//
LOCUS       DAOQLC010000101        15862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1338_length_15862_cov_21.649649, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000101 DAOQLC010000000
VERSION     DAOQLC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_15862_cov_21.649649"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..3228)
                     /locus_tag="PL000_15780"
     CDS             complement(19..3228)
                     /locus_tag="PL000_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90394.1"
                     /translation="MTENEAPTPAEILKSILEGLSGTEASVRLEAIEKLHSINYSSEA
                     IYIELERLAIHDDLEEVRKRALEALDLATHKHVRGRVNRLNAGDRTVLVEEIGEWESL
                     GLLDLTRAAVLRRRYDFDKRAKVTADAVMSEKPATVEKAEPAPKAEPAPAEPRPTLMQ
                     TLLSETSIKIALYLGAFFVVASAAILAAVSPELRLFVLIVASLGFGGLSLGIRKRLPQ
                     PSFALFIVFSFLLPITANVLQDTLGLTDVVKAVYWVVISLGMAVLWGGSIWLYESRVF
                     SVMSFLAFVLAFYRIGEVFEAEGEVYGLLMGAATLTGLGLVWVLKKWRDDKFALPLFL
                     STLAVQALILGGTLVLFAIQLFDGNDSALWNLASMLTWGLAFIFLVLADVLFPFVFFA
                     WFAALALVPIPWFLGAAFDMEFNGLAILFFVWGLILSVFSEAGFRLEWTRKFSLPVAM
                     VAIPAFVLALINAFSEGVSLGFGIALGTTLVYAVLQLLRGREWVWALALLNFFIAYFA
                     FFNLPLIEPLKVYFGYQALGLSLLLMLPGLFGKNDLRTASTWKLPTLAFGVIFTLTAT
                     VAFVIGENSLHAMVGFGVLAVFFTAYALVQGSAFYGYLPASFLALSALFGVEYLDKDW
                     WLPVLTTLAVLYYAFGAALLSRAGWSRMLRNSGLVLGTLIVVAAAAQMKGAGGWYAMV
                     VGLIFAVEMYLSGEGLLEIAPPIFFSAGVILVQRSIPTPMGGTHQYLVHSLVWLGWDL
                     LMHLTFKRARPLSWLVRGGGALVTVVNYLILMDMGSAKAGALDFGIYTLLFLTVNLVY
                     RRPILFHTFGVTLPLFVMFAFRLVGVEKWIHPVIFVAMGFYAVGFALRWMKLEWAKDW
                     DKALILEGLGWGMVISIAAPTLGGLDASIPVAFAATLWAAEAFALRDVRLGFPANGLY
                     LLAYFIILNELNVDQPQFYSMGAAALGMFQHYLLTRSGSKTGTFVMGMLSQLTLLGTT
                     YIQMVDSEKLIYFVVLFFQSIAVLVYGLVIRSRSLTFTPIVLVVLGVVTVLYTALKGL
                     NAVVLIGCTGVILLGLGIAAVLLRERIARLGERLSDWRA"
     gene            complement(3551..5020)
                     /locus_tag="PL000_15785"
     CDS             complement(3551..5020)
                     /locus_tag="PL000_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNC90395.1"
                     /translation="MSTPEWVKDAVFYQIFPDRFAKSSRNPAAALPFEPWDSAPTTHG
                     FKGGDLYGVLEKLDYIQELGCTAIYLNPIFASASNHRYHTFDYYNVDPLLGGNEALKK
                     LLKEMHKRGMRLILDGVFNHASRGFWQFHHVLETGDSSPYKDWFHFDHDRLTGKKHWG
                     AYPTPHEDKILQHEDSLTAIGYRAWWNIPALPKFNTKTPAVREFLFEVAEYWIKFGID
                     GWRLDVPGEIDDDEFWREFRRRVRAINPDAYIVGEIWHEAQRWLQGDQFDAVMNYLFT
                     SASLSFFAGQHLDMGVISQAGGIKDRVRAVNAYEFANEIDRILNLYPPDITSAQLNLL
                     DSHDMPRFLSCASGDKAALKMAWLLMFALPGAPCIYYGDEVGVDGRHDPECRKAFPWD
                     ESKWDKELLEFAKACTTLRTEHAALRRGEYKRLYAEGEVAAFMRSYKNEHLTMVFNTA
                     TEDRSIELHFEKKPRVLLGNASLDGNRVTIPARSGIVLK"
     gene            complement(5033..6649)
                     /locus_tag="PL000_15790"
     CDS             complement(5033..6649)
                     /locus_tag="PL000_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNC90396.1"
                     /translation="MTKRIFLSLLLFLLISSCAPATPTTAPAPTTVAPPKIWWSDAVF
                     YEIFVRSFNDSNGDGAGDFNGVTQKLDYLQELGITALWLMPIFPSPSYHGYDVTDYYG
                     VNPQYGTMDDFKNLVAEAHKRDIRIIIDLVINHTSDKHPWFREAKKDPQSPYRDWYIW
                     NETDLKYEGPWGQRVWHSSTTGFYYGIFEAFMPDLNYANPAVTEEMKKIAAFWLADVG
                     VDGFRLDAAKHLIEDGMKQENTNATHLWYQNEFYPAYKAVDPEAMTVGELFGDGLNTI
                     ANYIKNDQFDLAFNFQLAASFLEAVNTEKSGDLARTLQVSDKAIPDHQYATFLTNHDQ
                     NRVMTQLGGDVNKAKLAAFLMLTSPGTPFIYYGEEIGMQGKKPDENIRLPMQWTSGEN
                     AGFSITRPWRAPASDYVFINVDAQTGAIDSLLEHYRALIQLRNANPAMQTGNLISLKS
                     SHPSVYSALRINENGMFLILANLSKETISKYNINLNGAGLAESAYSVETMFGAGQAAG
                     PVGSGEAFQEYKPFENLDPYAMYVLKLISK"
     gene            complement(6718..7620)
                     /locus_tag="PL000_15795"
     CDS             complement(6718..7620)
                     /locus_tag="PL000_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC90397.1"
                     /translation="MTTNPISRFFRWFFYRSRGDSPFKTILIHATLIIACVIAVYPVL
                     RVVTISLRPNDTLLTTDLRIIPENATLDNYREVLFGDAEKNRQSDFFLWIWNSVSVVA
                     VTSVISVILAAISAYAFSRFRFPGRSAGLIFLLTTQMIPAGMLLLPLFIMIAKLKMIN
                     TPLGLIIAYAVTSVPLTIWTLKGYYDTIPVDLEEAALIDGASRLTVFTKIILPLSTPA
                     LAIAFLFSFMGGWSEYLVARVVLQKNEMFTWPLGIFTFAQQFTVSWGQFAAASVLIAI
                     PIMALFLYSSKWLISGLTLGSVKG"
     gene            complement(7631..9133)
                     /locus_tag="PL000_15800"
     CDS             complement(7631..9133)
                     /locus_tag="PL000_15800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC90398.1"
                     /translation="MESDNFLRRNLPNILWIAVIVSIYFVLSSLIAPFSDGITLSSKI
                     VGERLQEIGSFLFAILGGLAIIEIYFYQIFFRQPLNRTATIITRAIQVAMGLLTFTFI
                     IYFFNLTDLIKLGDSAIGKFVNMPVDSFANLQKSLISSKVATKDSAKMVAGIIFALGI
                     LVLYAIIQFTSKPRNSRVGLAYLLIMPAFIGVLFLIIYPFLFEIKLAFSNASLGTQAT
                     KNAQYSLLFGWENLKTLFTGTVAKDAKFFEVFWRTILWTVINVTFHVLGGMGLAILLN
                     RPLKFKGLYRTLLVIPWAIPTTIAAMALRSEFDSRYGLFNILLGNLNTWLTTVSQNTA
                     NIIGIGNGFAWLAATIGPVSWKQDPFWAFVSVLIANIWLGIPFMSVIILGGLQSISQE
                     YYEAAEIDGATRWQQFRNITLPLLRPVLTPAIILGVVWTFNKFDIIYLITQGGPQEKT
                     DILVSSMYKAAFQFYRYGFTAMFALVIFAILFIFTLFYLRVSDGLKSATE"
     gene            complement(9296..10615)
                     /locus_tag="PL000_15805"
     CDS             complement(9296..10615)
                     /locus_tag="PL000_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC90399.1"
                     /translation="MKTKLSLLFSLLVVASMVLTACGGGAVATEAPAVEEPAATEAPA
                     TEAPAATEAPAAEPITITFWEQEGEDVDVFIDGLIADFQAANPNITVERTHYENEALR
                     DQFQTASLAEAAPDVVRVPNDFAGPFSTLDIIAPVDALYDDAFLGQFFPGALDPAKVG
                     GTLWGVPDNYGNHLMLIYNKSLIAEPPATFEELITVSKELTAGDVQGFAYNLNEPFWG
                     AGFYGAFGGWPLDESDQPQFNNQAFIDYLTFVASLKTDGVVPAECDYACADTLMKEGK
                     AAMIINGDWSLGDYTTALGENLGVAPVPTINGNPYTEMTAGKYFMVSKAVLDDAARQE
                     AVTAFITYMTSAEVQKKWLDEFKRLPSNAEVAKDPSIASDPILAGSMADLAVGRGQPA
                     APEMRCAWDAWRPNLEGVMAGTVTPEDAAVAAQEAADACVATLGTTP"
     gene            complement(10720..11880)
                     /locus_tag="PL000_15810"
     CDS             complement(10720..11880)
                     /locus_tag="PL000_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNC90400.1"
                     /translation="MGLTRAAVTLIVNDLIENEVIFEAESRSIPSGRPPVVLEINPKR
                     GLVAAVDMGATHLNLAIADFSAKIIGEKSLPLDIRKGPQACMPEVISNLKALLSEYGV
                     SSPQLMAIGIGVPGPVITDAGMVVSPPIMPGWDGYPIRQTLEQEFGCAVSLNNDAELG
                     ALGEWAYGAGRGEKNLAFIKVGSGIGAGLIINQQIYGGTTGSAGEIGHITIDENGPLC
                     TCGSRGCLEAFAGGHAIALQAKKLVESGKRTLLSSIPVENLTAQEVAKAARRGDLPSQ
                     EIIMRAGTSIGIAIAGLVNLFNPGAVIIGGGVAQSGDLLTTSIRQAVRERSLRASERG
                     VHITTAMLGRRSSLMGATVQAVNIAIHDAIEKKNPVARDSFPSEIKQVFESN"
     gene            complement(12166..13266)
                     /gene="ugpC"
                     /locus_tag="PL000_15815"
     CDS             complement(12166..13266)
                     /gene="ugpC"
                     /locus_tag="PL000_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019292901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNC90401.1"
                     /translation="MASVSFQKVHKKFGDLTIINSLDIEVQDKEFLVLVGPSGCGKTT
                     ALRLLAGLEEITGGEIKIGDRLVNDVAPKDRDIAMVFQSYALYPHLSVYDNMAFGLKL
                     RKTPKEEIKRRVNEAADILGIQDLLQRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQMRAEISKLHQRLQTTFIYVTHDQTEAMTMASRIAVINKGDLQQLDTP
                     QNLYDRPNNLFVAGFIGSPSMNFFPAKIVKEGGKVMVDTEDFKVQIPENLAAPYKNLE
                     GKNVVFGIRPENIHDPEFAPNNIHGEKVSSKVDVTELMGNETLLYLVSGKNTYVGRVD
                     PRSKLRVGNQSQFIFDMDKFHIFDAETEKAIR"
     gene            complement(13321..14301)
                     /locus_tag="PL000_15820"
     CDS             complement(13321..14301)
                     /locus_tag="PL000_15820"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC90402.1"
                     /translation="MDRILNWGLLSTAKINRALIKPLNASKRTRLLAVASRNISSAEA
                     YAREWSIPRAHGSYEALLNDPEIDIIYNSLPNHLHAEWTIKALRAGKHVLCEKPLALS
                     LEEVDAMIAASKETGKVVTEAFMYLHHEQTARIKQIVDNGELGTLQLIKGAFSFTLSR
                     EGNYRWMTEMGGGSIWDVGCYPISFARTLVGAEPVEVFGWQVTGQGGSDDAFVGQMKF
                     EGGVHMQFDSSFRSPHRSYMEIVGSEAVLKISNPFKPGKDNNLILVRGDDETVIRVKG
                     GELYMGEVDDMCDAVLNGKPPHVSLEASRNNTAVILALLESAKSGKLVIV"
     gene            complement(14294..15454)
                     /locus_tag="PL000_15825"
     CDS             complement(14294..15454)
                     /locus_tag="PL000_15825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90403.1"
                     /translation="MQTTTSKNTILILTAGCFLSFFVFGFTDNLKGPTLPAMIAELNI
                     NYGTSGNIFFGQYLGFLITSFIAGVLADRFGLKTVLIIAGAFLFIGVTGYSSFQSAVL
                     LGVSLFLIGLGLGAIELGANAAIVEAHPEKRGLYLNLMAVMHGLGSLLAPLFAGWLLS
                     MNTNWRTIYRWDIALIALFIFLFSFVRFPKSKEHNSIDFKHIAQIAFKGNLPWFYFAI
                     ACYVAAEIGLSSWLVTFLQELRSASVTSSTQALSLFFTMLMLGRLGGGFIVQRLGYLR
                     AILFASVGALVCLAAGTFTNFSVLLPITGLFFSIIFPTFTAAVSIDHPESTNSILGIL
                     FTFAGLGGLIGPWAIAWASDLMGLQLGFSVGIIFTALLLWAIFVLEKGKRNG"
     gene            complement(15551..>15862)
                     /locus_tag="PL000_15830"
     CDS             complement(15551..>15862)
                     /locus_tag="PL000_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90404.1"
                     /translation="YVIFKADALPPFKMGYFNPHGWLAYWLDGVLFRKTFEVQTGSLH
                     PDNNCNAEIYCGDQFVELESLAPLEKLDTNASVTHTETWDIFTDLDSLPKEAQQLLAA
                     S"
BASE COUNT         4062 a         4128 c         4052 g         3620 t
ORIGIN      
        1 cctgaaccct gcctccccct acgccctcca atcgctcaac cgctcaccca accgcgcgat
       61 tcgctcacgc agcaacaccg ccgcaattcc cagaccaagc aagatcacgc ccgtacaacc
      121 gatcagcacc actgcgttca aacccttcaa ggcagtgtac aacaccgtca ccacacctaa
      181 aaccaccaac acgatcggcg tgaaggtcaa actccgcgag cggatcacca atccatacac
      241 caacaccgca atggactgga agaacagcac cacgaaatag atcaacttct ccgaatccac
      301 catctgaata taggttgtcc ccaacaaggt cagttgtgaa agcattccca tcacgaaagt
      361 ccctgtcttg ctccccgacc gcgtcagcaa ataatgctgg aacattccca acgctgccgc
      421 acccatcgaa tagaattgcg gctggtccac gttcaattca ttcaaaatga tgaaatacgc
      481 cagcaaatac aacccgtttg cagggaagcc caaccgcaca tctcgtaaag cgaatgcctc
      541 cgccgcccac aacgttgccg cgaacgccac agggatcgaa gcatcaagcc cgcccagcgt
      601 tggcgccgcg atcgaaatca ccatgcccca gcccaaccct tccaaaatca aagccttatc
      661 ccagtccttt gcccactcca gcttcatcca ccgcagcgca aaaccaaccg cataaaatcc
      721 cattgccacg aagatcacag ggtggatcca cttctccacg cccaccaaac gaaaagcgaa
      781 catcacgaac agtggcagag tcacaccaaa agtgtggaac aagatgggtc ggcgatagac
      841 cagattcacc gttaagaaaa gaagtgtata aattccaaaa tccagcgccc cagccttcgc
      901 gcttcccata tccatcagga tcaggtaatt caccaccgtc accaacgcac cgccgccgcg
      961 caccagccaa cttaatggac gcgcgcgctt gaacgtcaaa tgcatcaaca gatcccagcc
     1021 cagccacacc aggctatgga caagatactg atgtgtgccg cccatcggcg tcgggatgga
     1081 acgttgcacc aggatcaccc cagcgctgaa aaagatcggc ggcgcaatct caagcagacc
     1141 ctcgccgctc aaatacattt ccacggcaaa gatcaaaccc accaccatgg cgtaccatcc
     1201 gcccgcccct ttcatttgag ccgccgccgc aaccacgatc aaagtgccca gcaccaaacc
     1261 gctgttccgc agcatccgcg accagccagc gcgtgaaagc agcgccgccc caaaggcata
     1321 atacaaaacc gccagcgtcg tcaacacggg cagccaccaa tccttatcca gatactccac
     1381 cccgaacaac gccgaaagcg ccaaaaagct cgcaggcagg tatccataaa acgccgaacc
     1441 ctgcaccagc gcatacgccg tgaagaacac cgccaacact ccaaacccca ccatggcatg
     1501 gagcgaattc tcaccgatga caaaggccac cgtcgccgtc aatgtaaaga tcaccccaaa
     1561 cgccagggtc ggcaatttcc atgtcgaagc ggttcttaga tcgttttttc caaacagccc
     1621 gggcagcatc aaaagcaggc tcaacccaag tgcctgatat ccaaaataca ctttcaacgg
     1681 ttcgatcaac ggcagattaa agaacgcaaa ataggcaatg aaaaaattca gcaacgccag
     1741 cgcccaaacc cactcacgcc cgcgcagcaa ctgcaaaacc gcatacacca acgtcgtacc
     1801 cagggcgatt ccaaacccca gcgaaactcc ctcactgaac gcattgatca acgccagcac
     1861 aaaagcaggg atcgccacca tcgccacagg cagactgaac ttccgcgtcc attccaacct
     1921 gaaacctgcc tcgctaaaga cagaaaggat cagcccccaa acaaaaaaca agatcgccag
     1981 cccgttgaat tccatgtcga aggctgcccc caaaaaccac gggatcggca ccaaagccag
     2041 tgccgcaaac caggcaaaaa acacgaacgg gaaaagcaca tcggcaagca caaggaatat
     2101 gaaagccagt ccccatgtca gcatcgaagc cagattccaa agcgcggaat cgttgccatc
     2161 gaacaattga attgcaaata agaccagtgt cccgcccagg atcaatgcct gcaccgccag
     2221 tgtgctcagg aacagcggca gcgcaaactt atcatcccgc cacttcttca gcacccaaac
     2281 caacccaagc ccggtcagag tcgccgcgcc catcagcagg ccgtacactt ctccttccgc
     2341 ctcgaacacc tccccgattc gatagaaggc cagcacgaaa gccaggaagc tcatcacact
     2401 gaacacccgc gactcataca gccagatgct tccgccccaa agcactgcca tccccaatga
     2461 gatcaccacc caatacaccg ccttgaccac atccgtcagg ccgagcgtat cttgcagcac
     2521 attcgccgtg atcggcagca ggaacgagaa cacaatgaaa agcgcaaagc ttggctgggg
     2581 cagccgcttg cggataccca atgaaagccc gccgaaaccg agcgaagcca cgatcaacac
     2641 gaacaggcgc agctccggtg aaaccgccgc caaaatggcc gccgatgcca ccacaaagaa
     2701 tgcccccaaa tataaagcga tcttgatgct cgtttcgctt aacagggttt gcatcagggt
     2761 ggggcgcggc tcagcggggg caggctctgc cttgggtgcg ggttctgcct tttcaaccgt
     2821 ggcaggtttc tcgctcataa ccgcatcggc agtgaccttc gcccttttgt cgaagtcata
     2881 acgccttctc aacaccgcag cgcgcgtcag gtctaacagc ccgaggctct cccactcacc
     2941 gatctcctcc acaagcacag tgcggtcacc cgcattcaat cggttgaccc ttccgcgcac
     3001 atgtttatgg gtggcaaggt caagcgcctc caacgcgcgc tttcgaacct cctccagatc
     3061 atcatggatc gccaaccttt ccaactcgat gtaaatggcc tcactgctgt aattgatcga
     3121 atgcagtttc tcaatggcct ccagccgcac cgatgcttcg gtgccgctca agccttcaag
     3181 aatggatttg agaatttcag caggggttgg ggcttcattt tcagtcataa aaggtttcca
     3241 tgttttttat tttttggttt ctcgctcaaa ccgccgtcct cggcggcggg gacgccgccg
     3301 agagcatatt tttataaaat ggttcttcag gattaagtgt tcaccacgga gatttataaa
     3361 ggttttctct gagtctccgt gtctcggtgg taaaaaaagc aggagacccc accagacacc
     3421 aaagagcctg taaaatttac tacctgcctg acagttttcg gtaatcaaca aacttgaacc
     3481 actgaggcac cgaattttaa tggctttctt tgtgtttccg tgcctcagtg gttggtcttt
     3541 ttctttttgc ctacttcaaa acaattccac tccgcgcagg gatcgtcacc cgattcccgt
     3601 ccaaagaggc atttccgagc agtacccgcg gtttcttctc aaaatgcaat tcaatggagc
     3661 ggtcctctgt tgcagtgtta aacaccatag tcaaatgttc gttcttgtat gaacgcatga
     3721 acgccgcgac ttccccttcg gcatacaggc gcttgtattc gccgcgccgc agggctgcat
     3781 gttcggtccg cagggttgtg caggctttgg cgaactcaag cagctctttg tcccatttgc
     3841 tttcgtccca cggaaatgcc tttctgcatt cggggtcgtg ccgtccgtct acaccgacct
     3901 catcgccata gtaaatgcaa ggtgcgccag gcagggcaaa cattaacagc caggccatct
     3961 tgagcgccgc tttatctcca cttgcgcagg aaaggaagcg cggcatgtcg tggctatcga
     4021 gcaggttcaa ttgcgccgaa gtgatatcag gcggatacag gttcaggatg cggtcaattt
     4081 cattcgcaaa ttcatacgcg ttcaccgcac ggacacggtc cttgattccg cccgcctgcg
     4141 agatcacgcc catatccagg tgttgccccg caaaaaagct gagtgaagcc gatgtgaaga
     4201 gataattcat caccgcatcg aactggtcac cctgcagcca gcgctgggcc tcatgccaga
     4261 tctcgccaac gatgtatgca tcggggttga tcgcccgcac ccgtctgcgg aattcacgcc
     4321 agaactcgtc atcatcgatc tcaccgggca catccagccg ccagccgtca atgccgaact
     4381 tgatccaata ctcggcaact tcaaataaaa attcgcgcac ggcgggggtc ttggtgttga
     4441 atttcggcag cgcagggata ttccaccagg cgcggtaacc gatggcggtc aggctgtctt
     4501 cgtgctgaag gattttatct tcgtgggggg tggggtaggc gccccaatgc tttttacctg
     4561 tcaggcggtc atggtcaaaa tggaaccagt ctttgtaagg cgaggaatcc cccgtctcca
     4621 gcacatgatg gaattgccag aatccgcgtg aggcgtggtt gaacacaccg tccaggatca
     4681 accgcatacc gcgcttgtgc atttctttga gcagtttttt caacgcttca ttgccgccca
     4741 acaagggatc cacgttgtag taatcaaagg tgtgataacg gtgattcgaa gccgaagcaa
     4801 agatcgggtt aagatagatc gctgtgcatc caagctcttg aatgtaatcc aatttttcga
     4861 gcacgccgta caagtcaccg cctttgaatc catgcgtggt tggggctgag tcccacggct
     4921 caaacggcag tgcagccgca gggttgcggc tgctttttgc gaaacgatcg gggaatatct
     4981 ggtaaaaaac tgcatctttt acccattctg gtgtcgacat tatttatcct cgttactttg
     5041 aaatgagttt caaaacgtac attgcatacg gatcaagatt ctcgaacggc ttgtattcct
     5101 ggaaggcttc gccgcttcca actggacctg cggcctgccc agccccaaac atggtctcca
     5161 cactgtaggc ggattctgct aagcctgccc cgttgaggtt gatattatat ttgctgatcg
     5221 tttcttttga gaggtttgcc aatatcagaa acattccatt ttcattgatt cgcaaagccg
     5281 aatacaccga aggatggctg gatttcaacg aaataagatt acctgtctgc atggcggggt
     5341 ttgcgttgcg caattggatc agcgcgcggt agtgttcgag cagcgagtcg atggcacctg
     5401 tttgggcatc gacatttata aaaacataat cgctggcagg cgcccgccac ggacgagtta
     5461 tgctaaagcc cgcattttct cccgaggtcc actgcatggg caggcggatg ttttcatctg
     5521 gttttttccc ctgcatgcca atctcttcgc cgtaatagat gaacggtgtg ccgggcgagg
     5581 taagcattaa aaaggcggcc agtttggcct tgttcacgtc tccgcccaac tgcgtcatga
     5641 cgcggttctg gtcatggttc gttaaaaacg tggcgtattg atgatcgggg atggccttgt
     5701 cgcttacttg cagagttctg gcgaggtcgc ctgatttttc agtgttgacc gcttcgagga
     5761 aactggccgc aagttgaaag ttgaaagcca ggtcgaattg atcgttcttg atgtaattgg
     5821 cgatggtatt taatccgtcg ccgaacaact caccgacggt catggcttca gggtcaactg
     5881 ctttgtatgc gggatagaat tcattttgat accaaagatg agtggcattg gtattttcct
     5941 gcttcatgcc gtcttcgatc aggtgtttgg cggcatccag gcggaagcca tccacgccca
     6001 catcggccag ccagaaggcg gcgatttttt tcatttcttc ggtaacagcg gggtttgcgt
     6061 aattcaggtc aggcatgaag gcttcaaaga tgccgtaata aaaacccgtt gtagaggagt
     6121 gccagacgcg ctgcccccac gggccttcat atttcaggtc tgtctcgttc cagatatacc
     6181 aatcgcgata gggcgattgg gggtcttttt ttgcttccct gaaccagggg tgtttgtcgg
     6241 atgtgtgatt gatgaccaga tcaatgatga tgcggatgtc gcgtttgtgg gcttctgcca
     6301 ccaggttttt gaagtcatcc atcgttccat attgcgggtt tacaccgtaa tagtctgtga
     6361 cgtcgtaacc gtgataggag ggggagggga aaatgggcat cagccagagt gcggtgatac
     6421 caagttcctg tagatagtcc agcttttggg tgacgccgtt gaaatcaccc gcgccgtcgc
     6481 cgttgctgtc gttgaaagag cgcacaaaaa tttcgtagaa cacggcatcg ctccaccaaa
     6541 ttttaggagg agcaactgtc gttggcgctg gagcagttgt cggcgtggcg ggagcgcagg
     6601 aagaaatgag caggaaaagc agcagggaga gaaaaatccg tttggtcatg acagtcaata
     6661 gacaaaaata ttcccttcct ctcaaggaaa gaaagggaat attcaatgat gaaagtatta
     6721 tccctttacg ctgccaaggg tgagacctga gatgagccac ttggaagaat agaggaagag
     6781 cgccatgatg ggaatggcga tcaaaaccga tgcggctgcg aattgacccc acgaaaccgt
     6841 gaattgctgt gcgaaggtga agatgcccaa cggccaggtg aacatttcat tcttttgcaa
     6901 aaccacgcgt gccaccagat attcgctcca gccgcccatg aaggagaata ggaaggcaat
     6961 cgccagtgcg ggtgtggaga gaggcaggat aatcttggtg aatacggtca ggcggcttgc
     7021 gccgtcgatc agcgcggctt cttccaggtc aacgggaatt gtatcgtagt agcctttgag
     7081 ggtccaaatg gtgagcggca cggaggtcac tgcataggcg atgatcagac ccagaggcgt
     7141 attgatcatt ttcaatttgg cgatcatgat aaataaggga agcaacaaca tacccgcagg
     7201 gatcatctgc gttgtcagca ggaatataag ccctgccgag cgcccgggaa aacggaagcg
     7261 cgaaaacgca tacgcgctga tcgctgccag aatgacgctg atcactgaag tcaccgctac
     7321 aacagagacc gagttccaaa tccagaggaa gaaatcggac tggcgattct tttccgcgtc
     7381 gccgaacaat acttccctgt aattgtccag cgtggcgttt tctgggatga ttctgaggtc
     7441 ggtggtcagg agtgtatcat tggggcgcag cgaaatggta accacgcgca agacgggata
     7501 tacagcgatg acacaggcga tgataagggt cgcatggatc aaaatggttt tgaagggact
     7561 gtcgccgcgt gaccgataaa agaaccagcg aaagaagcgg gaaattggat tcgttgtcat
     7621 gtgatgctcc ttattcagtt gcggacttaa gcccatcgct gaccctaaga taaaagagag
     7681 tgaagataaa caggatcgca aagattacca gtgcgaacat ggcagtgaag ccgtaccgat
     7741 aaaactggaa tgcggcttta tacatggagg aaaccagaat atccgttttc tcctgcgggc
     7801 cgccctgcgt gatgaggtag atgatgtcga acttattgaa ggtccagacg acgcctaaaa
     7861 taatggcagg tgtcagcacg gggcgcagta gagggagggt gatgttacgg aattgttgcc
     7921 atcgggtggc gccgtcgatc tctgcggctt cgtaatattc ctgcgaaatg ctttgcaacc
     7981 cgccgagaat gatgacggac ataaaaggaa tgcccagcca gatatttgcg attaacaccg
     8041 aaacgaacgc ccaaaatggg tcctgtttcc aactgacagg gccgatggtg gccgccagcc
     8101 aggcgaagcc atttccgatc cctataatat ttgccgtgtt ttgactgacc gtggtcaacc
     8161 aagtgttgag gttgcccagc aggatattga acaagccgta gcggctgtca aattcactgc
     8221 gtaatgccat ggctgcaatc gtggttggaa tcgcccaggg aatgacgagc aaggtgcgat
     8281 aaagcccttt gaacttcaag ggtctgttca gcaggatggc cagccccata ccgcccaaaa
     8341 cgtgaaaggt gacgttgata accgtccaca ggatcgtgcg ccagaacacc tcgaagaatt
     8401 tcgcgtcttt cgcgaccgtg cctgtgaaga gtgtcttgag attttcccac ccgaaaagca
     8461 gactgtattg ggcattctta gtcgcttggg ttccaagcga tgcattggag aatgcgagtt
     8521 tgatctcgaa aaggaagggg tagatgatca ggaagagcac gccgataaaa gcgggcatga
     8581 tcagcagata ggccagcccc acgcggctgt tgcgcggttt gctggtgaat tgaatgatcg
     8641 catacagcac caggatgccg agggcaaaga tgatgcctgc gaccattttt gccgagtctt
     8701 ttgtcgcaac ctttgagctg atgagggact tttgaaggtt cgcaaacgag tccacgggca
     8761 tgtttacaaa cttgccgatg gcgctgtcgc cgagtttgat taggtcggtt agattgaaaa
     8821 agtaaatgat gaacgtgaag gtgaggagac ccatcgcaac ttggatggct cttgttatga
     8881 ttgttgcggt gcggttaagc ggctggcgga agaaaatttg ataaaaataa atttcgatga
     8941 ttgcaagtcc gcccaggatt gcgaaaagaa agctgccaat ttcctgaagc cgctcaccta
     9001 cgatcttgct gcttaaggtg atgccgtctg agaagggagc gatcaacgat gatagaacga
     9061 aataaatcga gacgattaca gcgatccata ggatgttggg caggtttcgt cgcaggaaat
     9121 tatcgctttc cataataaac ctcttttgtt tgctcaccat ggggaacagg gcgagtcttc
     9181 aagaatatca gcgcaattcg cggccaagtg tggatattat aaagcttgaa gcttaagtag
     9241 attgaaaata ggatggggag cgtcacgctc cccatcctat tttattacag gaatactatg
     9301 gggtggttcc gagggtggct acacaagcat cggcagcttc ctgagcggcg acggcggcat
     9361 cttcaggggt gacggtgccg gccattacgc cttcgaggtt cgggcgccat gcatcccatg
     9421 cgcagcgcat ttcaggagcg gcaggctgac cgcggccaac ggcgaggtcg gccatggagc
     9481 cagcgaggat cgggtcactt gcaatgctcg gatccttggc aacttcggcg ttggagggaa
     9541 ggcgcttgaa ttcatcaagc cacttcttct gaacttcagc ggaagtcatg taggtgatga
     9601 acgcggtgac agcttcctgg cgggcggcgt catcgaggac ggccttggag accatgaagt
     9661 atttgccagc ggtcatttcg gtgtaggggt tgccattgat ggtggggacg ggggcaacac
     9721 cgaggttttc accgagagcg gtggtgtaat cgccgagaga ccagtcgcca ttgatgatca
     9781 tggcagcttt gccttccttc atcagggtgt cggcgcaggc ataatcacat tcagcgggca
     9841 caacgccgtc ggttttgagg gaggcgacga aggtcagata atcgatgaaa gcctggttgt
     9901 tgaactgagg ctgatcgctc tcatcaagag gccatccgcc gaaagcgcca tagaagccag
     9961 cgccccagaa aggctcattg aggttgtagg cgaaaccttg aacgtcacca gcagtgagtt
    10021 ccttggaaac ggtgatgagt tcttcgaaag tggcaggggg ctcagcgatc aaggatttgt
    10081 tatagatcag catgaggtga ttgccgtagt tgtcggggac gccccaaagg gtgccgccaa
    10141 ctttggcagg atcaagagcg ccggggaaga actggccgag gaaggcatca tcgtacaacg
    10201 catcaacggg ggcgatgatg tcgagggtgc tgaaaggacc agcgaaatcg tttggaacgc
    10261 gaacgacatc gggggcggct tcagcgaggg aagcggtctg gaactggtcg cggagagctt
    10321 cgttctcgta gtgagtgcgc tcaacggtga tgttcgggtt ggcagcctgg aaatcagcga
    10381 tcaaaccgtc gatgaacacg tctacatctt cgccttcctg ttcccagaaa gtgatggtga
    10441 tgggttcggc agcgggggct tctgtagcag caggagcttc ggtagcgggg gcttcagtgg
    10501 cagcgggttc ttctacggcg ggtgcttcag ttgcgacagc tccgccaccg caggcagtga
    10561 ggaccatgct ggccaccact aacaagctga acaacaaact taatttggtt ttcattactc
    10621 ttctccttac ttaataggtg attatgttga ttggtattga acgatcattt catgatttgt
    10681 gcatgtttgt ttttctgagt gcaggcgatc acctcctttt cagttacttt caaatacctg
    10741 tttgatttca gacgggaatg agtctcgcgc aacaggattt ttcttttcga ttgcatcgtg
    10801 aatggcaata ttcactgcct gcacggtggc acccattaat gacgaccttc ttccaagcat
    10861 ggcggttgtg atatggaccc cgcgctcgga ggcccgcagt gaacgctccc gcaccgcctg
    10921 acggatcgaa gtggtcagca aatctcccga ttgtgcaaca ccgccgccga taataaccgc
    10981 acctgggttg aacaaattaa ccagacccgc aatggcaatg ccgatggacg ttccagcgcg
    11041 cataatgatc tcttgcgaag gcaggtcgcc acgccgtgcg gctttggcta cttcctgtgc
    11101 agtcaggttt tcaacgggaa tactggaaag caaggttcgt tttccagatt cgaccaattt
    11161 tttagcttgc agggcgatcg catgtccgcc agcaaacgcc tccaaacagc cacggctgcc
    11221 acaggtgcaa agcggaccat tttcatcgat cgtgatatgc ccaatttcac cagcagaacc
    11281 ggttgtccca ccgtaaattt gttgattgat gatgaggcct gcgccaattc cgctgcctac
    11341 tttgataaaa gcgagatttt tctcgccgcg gcctgctcca tatgcccatt cacccaaagc
    11401 acccagttct gcatcgttgt taagagaaac tgcacagcca aattcctgtt caagggtctg
    11461 acggatcggg tagccgtccc agcctggcat gatgggtgga gaaaccacca tccctgcatc
    11521 tgtaatgacc gggcccggaa caccgattcc gatggccatt aactggggtg aggaaacccc
    11581 atactcactt aacaatgctt tcaaattgct gattacttca ggcatacaag cctgagggcc
    11641 ttttcgaata tccagcggta aggatttttc accgataatc tttgccgaaa agtcagcgat
    11701 tgctaggttt aggtgggtgg cgcccatatc cactgcggca accaagccac gctttgggtt
    11761 gatctccagc accactggag ggcgtccact tggaatggat ctgctttctg cctcaaaaat
    11821 gacctcattt tcgatcagat catttacgat caaagtcact gcggcacgtg ttaaacccat
    11881 ttgttctgca agatctgttc ttgaaatgcc gttgagggcg aatcgaatca aatctaccgc
    11941 agaatgtttg ttaaaattct taatattttg attggtgggt atccaataaa acatgatttt
    12001 ttaggctaca aaactttttt ggtttcaaaa ttaccttttc tataatagca caaaatagta
    12061 ttaaatcaag tgttttaaca aaattcatgc ttaaaatttc ttccctaaac taaaaaggac
    12121 tggagctaca aactccagtc cttttttatg ttatttcttg acttcttagc gaatggcttt
    12181 ttcagtctct gcatcgaaaa tatggaattt gtccatatca aagatgaatt gcgattgatt
    12241 tccaacgcgc agcttcgagc ggggatccac acggccaaca taggtgttct ttccgcttac
    12301 cagataaagc agggtttcgt tacccatcaa ttcggtcaca tccaccttgg aggaaacttt
    12361 ctcgccatgg atgttattcg gggcgaattc tgggtcatga atattttcag ggcgaatgcc
    12421 gaagacaaca ttcttgcctt ccaggttctt gtagggggcc gcaaggtttt caggaatttg
    12481 aaccttgaaa tcctcggtat ccaccattac tttgccgcct tctttgacaa tcttggcagg
    12541 gaagaagttc atggacggag agccaatgaa accagctaca aacaggttgt tggggcggtc
    12601 atacaggttt tgcggagtgt ccaactgttg caaatcacct ttgttgatga cggcgatgcg
    12661 tgaagccatg gtcatggctt ccgtttgatc gtgggtcacg tagatgaatg tagtttgcag
    12721 gcgttgatgg agcttgctga tttcagcacg catctgcacg cgcaattttg catcgaggtt
    12781 ggagagcggc tcatcgaaca ggaagacctt tggctcgcgg acgatggcac gacccacagc
    12841 cacacgctgg cgctgaccgc ctgataactg gcgcggcttg cgctgaagca gatcttgaat
    12901 gcctaaaata tctgcagctt cgtttacgcg acgcttgatc tcttccttcg gggtcttgcg
    12961 aagcttgaga ccaaaagcca tattgtcgta cactgaaagg tgcggataca acgcgtagga
    13021 ttgaaatacc atggcaatat cgcgatcttt aggcgccaca tcgttcacga ggcgatcacc
    13081 gatcttgatt tcgccgccgg tgatttcttc caaaccagcg agcagacgca gggcggtggt
    13141 tttaccgcaa cctgatgggc cgactaagac aaggaattcc ttgtcctgaa cctcgatatc
    13201 gaggctgttg atgatcgtca gatcaccaaa cttcttatgc actttctgaa atgaaacgct
    13261 tgccatggta tcctccagtc aaaagaatat agtgactgaa ttataaaccc gcaaaacaac
    13321 ttagacaata accagttttc cgcttttggc tgattcgagc agagccagga ttacagcggt
    13381 gttgttgcgg ctggcctcca gcgaaacgtg aggaggcttg ccattcaaca ctgcatcgca
    13441 catatcgtcc acttcaccca tgtataactc gccacccttc acccgaatga ccgtttcgtc
    13501 atcgcctctg actagaatca ggttgttatc cttaccgggt ttgaatgggt tggaaatttt
    13561 caaaaccgcc tccgaaccga cgatttccat gtaagaacga tgcggcgacc ggaaactcga
    13621 atcgaactgc atatggactc caccctcaaa cttcatttga cctacaaaag catcgtcgct
    13681 tccaccctga cctgtaacct gccagccaaa tacttcaaca ggctcagcgc cgacaagggt
    13741 acgggcaaaa gagatcggat agcatcccac gtcccaaata ctcccacctc ccatttccgt
    13801 catccaacgg taattgccct cacgcgaaag tgtgaaagag aaagcccctt ttataagctg
    13861 caaggtgccc aattcgccat tgtctacgat ctgtttgatc ctggccgttt gttcatgatg
    13921 aagatacatg aatgcttcgg ttaccacttt gccagtttct tttgaggcgg caatcatggc
    13981 atccacttct tcgagtgaga gagcaagagg tttttcacac agcacatgtt tgccagcgcg
    14041 caacgccttg atcgtccatt cagcgtgcag gtgattgggc agggaattgt aaatgatgtc
    14101 aatttccggg tcattgagca gggcttcgta actgccgtgc gcgcgcggaa tactccattc
    14161 tcgagcgtat gcttcggctg aggaaatgtt tctcgaagca acggccagca atcgggtccg
    14221 ttttgatgcg ttgagaggtt tgatcaaggc gcggtttatt tttgcggtgg agagcaatcc
    14281 ccaattaaga atcctatcca tttcgttttc ctttctctaa aacaaagatt gcccagagca
    14341 ggagggctgt gaaaatgatg ccgaccgaaa aaccgagttg caaccccatc aggtcgcttg
    14401 cccatgcaat ggcccacgga ccgatcaacc cgcccaaccc tgcaaaagtg aagaggatgc
    14461 ccaggattga gtttgtactt tcaggatggt caatggatac tgccgcggtg aaggttggga
    14521 agatgatcga gaaaaaaagc ccggtgatgg ggagaagaac actgaagttt gtgaatgtgc
    14581 ctgctgccag gcagaccaat gcgccgaccg atgcaaataa aatcgcccgc agatacccaa
    14641 gccgttgaac aataaaaccg ccgccgagcc gtcccagcat tagcatggtg aaaaataatg
    14701 aaagtgcttg cgtgcttgat gtaacacttg cactgcgtaa ctcctgcaga aaggtgacca
    14761 gccaggagga taatccgatt tcagcggcga cgtagcaggc aattgcaaaa tagaaccaag
    14821 ggagattgcc tttgaaggcg atctgggcaa tgtgtttgaa atcaatactg ttgtgttcct
    14881 ttgattttgg aaagcgcaca aaagaaaaaa ggaagatgaa aagggcgatc agggcgatgt
    14941 cccagcgata aatcgtgcgc cagttggtgt tcatggataa caaccaacca gcaaaaagcg
    15001 gtgcaagcag agatcccagt ccgtgcatga cggccattaa atttaggtag aggcctcttt
    15061 tttcaggatg tgcctctaca atggcggcgt ttgcaccaag ctcgatggcg cccaaaccca
    15121 acccgatgag gaaaagtgaa actcccaaca gaactgcgct ttgaaatgag ctgtatccgg
    15181 tgacgccaat gaaaagaaag gcccccgcga tgatcaggac ggttttcaac ccgaaacggt
    15241 cagcaagtac accagcaata aaggatgtga tcagaaagcc gagatattgg ccgaagaaaa
    15301 tatttccact tgtgccgtaa ttgatgttca gttctgcgat cattgcaggc agagtggggc
    15361 ctttgagatt gtcggtaaaa ccgaagacga aaaaggagag gaagcatcca gccgtcagaa
    15421 ttaagatcgt gttttttgag gttgtggttt gcatgatgga ggaaattata ctggttgaag
    15481 ctgggctttt ttctgtgtca ggctgggaaa agaaaactcc cggtgttttg aaatttcacc
    15541 gggagcaggc ttatgaagct gccagtagtt gttgtgcttc tttgggcagt gaatctagat
    15601 cggtaaagat atcccatgtt tcggtgtgag ttacagatgc gtttgtatcc agtttttcaa
    15661 gtggagcaag gctttccaac tctacaaatt ggtcaccgca atatatttcc gcattgcaat
    15721 tattgtcggg gtgcagcgat cctgtttgga cttcaaacgt cttgcggaag agaacgccat
    15781 ccagccaata ggccagccag ccgtggggat tgaagtaacc cattttgaag ggcggcaatg
    15841 catcagcttt gaaaatgaca ta
//
LOCUS       DAOQLC010000102        15244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1418_length_15244_cov_22.502008, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000102 DAOQLC010000000
VERSION     DAOQLC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1418_length_15244_cov_22.502008"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..2613)
                     /locus_tag="PL000_15835"
     CDS             complement(22..2613)
                     /locus_tag="PL000_15835"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNC90405.1"
                     /translation="MVAKSAEPKVKNGLLPTPAMAKGLPKASLTDNARQVLMKRYVRR
                     GDDGKPAENVEEMFWRVAHHVAKVEEQWGADVAQRTVEYYHLLSSKKFFPNSPTFTGA
                     GTPLGQLAACFVLPITDDMGRDGAGIFQTLRDAALIQQTGGGNGFSFSRLRPKGALVQ
                     TSAGQATGPVGFLRVYDHAFGEIAQGGTRRGANMAVLRVDHPDVEDFVTCKINENSIT
                     NFNISVGITDAFMQAVKDDAEWPLRFPDLGEMKQKGFSGTLEQAEAAGITIHTYRKVN
                     ARELYNKIVKQAHHNGEPGVLFLDAANRGNPVPHLYPLEATNPCGEQWLGPYENCCLG
                     SVNLNEHCGPDGSVDWETLRQSVVTATRFLDDVVEANAYVPAVPQLKEAAHRARRIGL
                     GIMGLADLMYHAGVRYGSKEGQEFGAQVMEFVRFHAMKTSVELAEERGPFPAIEGSIY
                     DMDDMKWHAPKSLVDYQHNWGRPEVKWDAIVSGIKQHGIRNAAQTTVAPTGTIATVAG
                     CEGYGCEPVFALAYIRHVNDNGKDLKLTYASPRFDEALKKLGLGEEKRQEIVEQVMNE
                     GTCQNISEIPQAVRDTFVVSGDITAEEHVRMQGALQAFVDNSLSKTVNFHENATEEDV
                     AKAYMTAWELGCKGITVYVTGSRDKVVLETKATADKKAPASAEPKTVSSPQGAPVQQA
                     APTIWHGTKKPRPKALTGKTYNIETPVGKAFITINENGGTQPFEAFVNTAKAGSEIAA
                     VSEAIGRLISYILRMASPIAPVDRMREIVVQLIGIGGGRSLGFGINRVRSLPDGIGQV
                     LDQYLREKDGAPVEEVKTNGNGNGHHEEPQAHTMKIGDICPECGEAAVVNEEGCRKCY
                     ACGFSEC"
     gene            complement(2775..3224)
                     /gene="nrdR"
                     /locus_tag="PL000_15840"
     CDS             complement(2775..3224)
                     /gene="nrdR"
                     /locus_tag="PL000_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNC90406.1"
                     /translation="MRCPYCKHHDSKVLDTSHDSHGGIRRRRECLKCRQRFSSYERPI
                     LATPLIIKQDGGREEFDREKLARGIRISCAKRPVSAADIERMIGQVETQLQKMGKAEV
                     SSRVVGDLVMTTLKEVDQIAYIRYAIVYLGLDDLQSIRTEIDQLLED"
     gene            3531..4787
                     /locus_tag="PL000_15845"
     CDS             3531..4787
                     /locus_tag="PL000_15845"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC90407.1"
                     /translation="MMRIRLRLPVFAALITVFLIAQLVQPSQTWMFLLTVFSGIFISA
                     YLWTRSLGMSLQLRRETRLGWIQVGGQIEERITLSNTSVLPAAWIQFKDHSTLPEFHA
                     SRSTSISAGFFDQWIVTADSNQRGLFYLGDADIVTGDPFGIFEATIHASERTSILVLP
                     QVLTLPEFSILPSGSFGDGQPRRNAPEQTIHASTVREYVHGDSVRMIHWPTTARTNKV
                     FVRLMESAPAGDWWILLDLDKKNLFGTSWDSIEEQSVMLAASLASRGLRDRKAVGLVT
                     NSKDPAWIHPQKGEGQRWEILQSLALAQPAELTLTQTLERIQPLLGRRHSLIIVTASP
                     KPDWLKSLPPLIKSGIVPTVFLFDAATFGGNESPASVAANLDGLGIKYHLISHGLLEP
                     PKVLPRQQGGWKWRSTPTGEIVPIRS"
     gene            4797..7028
                     /locus_tag="PL000_15850"
     CDS             4797..7028
                     /locus_tag="PL000_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNC90408.1"
                     /translation="MKFFSLRSFLTWLILLLAMGALTAGLKDGILDVQDAAFFSVAAF
                     TVTLAFLIGFGTSSPRRAWSIVLVSGVLFAVIESARLMEPFKLIIRAIPPFERDLLLW
                     FIERDSPEAIFPDASIFLIQFTELVRRADTFFTLIFNAALARPMVREFIWDLPILFCA
                     AWAGWKTSRSNQALLALVPALVLQTFILEYTGKDLFPLQLGIFTLLMLIGVNHNWSIA
                     SGNGKTARETYSAILILSIALTMGSGFVPSISISEAAKKLTRKDDIGKALGLDKESVQ
                     AYVISGLSGLPRQHLIGMSPTLAETVMFTVQTGELPPTENSIIAQAIPRHYWRWLTYD
                     VYNGQGWSTSEVSSVTYSAGETILREGASFQTLHQQVKKSFSEDNRLYWAGQLGGVDQ
                     PVSVNWRDPLSEADMLGGTTQAQIYSADSLLVIVSADELRASPVVPITGPQEKYLSLP
                     VTVPQRVHALAESLTADFNNPYDKAKAIERYVRTYPYSLDVTPPAPNQDVADYFLFDL
                     KKGYCDYYATAMVVLARSVGLPARLVVGYANGIYNARSAQYTIREADAHSWVEIYFTG
                     VGWVEFEPTAGQLELNLPDDLSEAETLPPPVFPEPVSRLRDFDKQGFFPERNLLPPIL
                     AAVLSVLFVGIWFLYVQGLLRRHETIGSIYEYVYQHGRKIYDASPANETPTVFAQKLA
                     ARLKSGNEWIAPAPDEVRFLTALYLQETYSAHPVSQDERREAIKVWRKLFWRLLYAQF
                     KRI"
     gene            complement(7038..7664)
                     /locus_tag="PL000_15855"
     CDS             complement(7038..7664)
                     /locus_tag="PL000_15855"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC90409.1"
                     /translation="MKTRLILFSTLILGALLFSCESNKSTPTQPVELDTDTLRTEAVS
                     TFASALTETLAAVPTASPAPTEKFTATPVVLTATLETPAAADPCYDLIWIEDVTIPDG
                     TNLKPNETFTKTWLVQNSGGCAWPPGFTFQNVGGDPMRGETFILSEAIPVGAKREISI
                     QLAVPTDQNGLIQSSWRMADANGIFFGDTLTVNITVGNILTPTSTTSP"
     gene            7789..8760
                     /locus_tag="PL000_15860"
     CDS             7789..8760
                     /locus_tag="PL000_15860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC90410.1"
                     /translation="MNAVEVNQLRRIYRAQIGVFNRSIKEVVAVDEISFDVKQGELFG
                     LLGPNGAGKTTTVKMLTTLLIPSLGSASVAGFDVVKQANEVRKRIGFIFGGERGLYWR
                     LSGDDNLRYFASLYGVDPDVSKKRIPYLMELVGLKGRGNERVGGYSRGMKQRLHVART
                     LLHDPDILFLDEPTIGLDPVGAREFRAVILDLQAQKKTILLTTHYMFEADALSDRIAI
                     INKGRVIALDTPGGLKQHVSDLSVVEVETFGAPDSTLEKLRSLPFIDSLSVEDREQKQ
                     MLLVQTKRGAEAVPDVMAALDGQRIGRVIVREPTLEDAYVRLVGGQS"
     gene            8757..9554
                     /locus_tag="PL000_15865"
     CDS             8757..9554
                     /locus_tag="PL000_15865"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90411.1"
                     /translation="MNYANLFRYWRTVLTVAEMSIRTQFSDSFILFAIVFQPMIIAIL
                     ALFMLREKGGDYAMFVVVGSGLTGLWSSLLFVSGNSINSERWTGTLESLVGVPTPFDV
                     IVFGKNLANVFQSLLSMVTSYTLAAFLFGYSLSFTQPLLFMVTLLFSMFAFICFGLTI
                     APVFVMYRSVQQWQNAMEFPVYILGGFLFPIAMLPGWATPISYLLPPYWAAQALHGTS
                     TAMMPFEQILFCWGMMIVFSVVDLVIASQLFKVMLYKARVDATLDVD"
     gene            9557..10357
                     /locus_tag="PL000_15870"
     CDS             9557..10357
                     /locus_tag="PL000_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90412.1"
                     /translation="MNTIRKNLYLFWQGTIISYRALFAWLRPVTYVASKVLMPLAQMF
                     FFTFLGSYATNGKNIDFYVIGNAIQIASVSGIFGVTMSVGGDRDAGTLPYLFGTPANR
                     FMIFFGRAFMHVIDGALGVVIALTWGVLLMGLDLSRTDLPALGLTILITTFSTCGLGL
                     LMGCLSLLTANVMFVNNFVYFSLLVFSGANVPIATLPEWMQAVSTILPLTRGIASARA
                     LAAGASLSEVSPLLLSEAGIGLFYGVIGYFLFSWFEIQAKQKGTLETV"
     gene            complement(10688..11050)
                     /locus_tag="PL000_15875"
     CDS             complement(10688..11050)
                     /locus_tag="PL000_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90413.1"
                     /translation="MKSKWIEHNGKKILYQDFSNLFFNTQAVAEELSQVQQVVIGEPE
                     NSVLVLSDFRNTEVTNALMPLLNEASRNTKSHVRKTATLGITGVKRTLGDLLSRITGQ
                     PIMYFENEKDAKEWLVKD"
     gene            complement(11066..11473)
                     /locus_tag="PL000_15880"
     CDS             complement(11066..11473)
                     /locus_tag="PL000_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90414.1"
                     /translation="MKIRDASGWTIFIFGALAFILGLMGLIRPEILLSILGFEVIDRA
                     ARASSDYTIVFMTASSMASLNIGIYYVLAAFNDVKIFYRWTVPFRTLTFIVFTTTVIT
                     GIAPMRFFGVGLWEFLGALSTGIALYREDRAKK"
     gene            11584..12228
                     /locus_tag="PL000_15885"
     CDS             11584..12228
                     /locus_tag="PL000_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC90415.1"
                     /translation="MAKHLVLVAGNIGAGKTTLTERIGARLGWWTGYESVTDNPYLSD
                     FYGDMHAWSFHLQIFFLGHRADQYLEASRDARSAILDRSIYEDAHIFARALHHMGNLG
                     ERDYLAYRRLFEVVVNGLPRPDLLIYLKAPVPTLMDRIRRRARNMETGITPDYLSLLD
                     SFYSEWLSAFDLCPVLTIQTDDLDFAHHPGHLDLVVQRIQDKLAGKEVMDLTQK"
     gene            12230..13471
                     /locus_tag="PL000_15890"
     CDS             12230..13471
                     /locus_tag="PL000_15890"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNC90416.1"
                     /translation="MSNTLLSKIPLFAGLPRKELDQILAAMEKKEMADREILFKEGDP
                     GEHFYIVMKGNLEVLMAAGQAEELLLNVMSEGEHFGEMGLIMPGGHRTATVRARGEST
                     LLSMSRETFLGLTQKHPELSGSMVRVLSQRLDATNTQTFHELTEKNRKLQTAYDELKA
                     AQAQIIEKERLERELQVAAEIQLSILPDKLPEPPNFDFGARILPARQVGGDFYDVFYV
                     GEGLIGVLIGDVADKGVPSALFMSRAHALVMAEADTGLSAGEVMQLVNRHITRLQKST
                     QFVTVLYGILNLTTNEFSYARAGHEPPLILQPDGTVERLPYRPGMALGLWDSILLDEH
                     AITLPPGSTLLLFTDGMTDCRDPNGVAFGLDNIKSVLETLPAASAQQVCDHMLDTLVK
                     YQNGSKQDDDVTLVAIRATGK"
     gene            complement(13554..14690)
                     /locus_tag="PL000_15895"
     CDS             complement(13554..14690)
                     /locus_tag="PL000_15895"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010081119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HNC90417.1"
                     /translation="MKFETLAIHAGQEPDPNNGAVMTPVYLTSTYMQDGIGKPRQGYE
                     YSRTLNPTRKALQDCLAELESGQWGLAFASGMAATDTVLRMLGSGDHVVAGNDVYGGT
                     FRLFDKVLRRFGLDFTFADTTDPENLAEALTPSTKIVWLETPTNPLLAVSDIRAIAEV
                     VKHHPNKPLLVVDNTFATPYLQRPLEMGADLVVHSMTKYLGGHSDVVGGAIVGKDKEL
                     GEKLAYLQNAIGGIQGPMDSFLVLRGIKTLPIRMDRHAQNAERIVAFLEKQKNVKQLI
                     YPFHESHPQVQVAKRQMLNGGGMISFVMKDGRDAAVKVAESTKIFTLAESLGGVESLI
                     EVPAAMTHLSTQGSKLEVDPGLVRLSVGIENVEDLLGDLEQALR"
     gene            complement(14747..15190)
                     /locus_tag="PL000_15900"
     CDS             complement(14747..15190)
                     /locus_tag="PL000_15900"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90418.1"
                     /translation="MITYRSYEKKDWQAIFQIHDRARPDELIGSCDPRGFIPIEQDEE
                     VDDLKRANKFVACDDETVVGFVGVDEDYLAWLYVDPSHYRKGIGRELLKIGIREIGEG
                     AWTVALDGNKKAIALYESEGFVEIERFAGDNAGYPCMCLKMKRVK"
BASE COUNT         3348 a         3967 c         3975 g         3954 t
ORIGIN      
        1 caacctgcaa ccttcaaccc actaacattc cgaaaaccca caggcgtagc acttgcggca
       61 gccttcttca tttaccaccg ccgcttcacc gcattcggga caaatgtcgc cgatcttcat
      121 ggtgtgggct tgtggttctt cgtggtgacc gttgccgttt ccgttcgttt tcacttcttc
      181 cacgggggcg ccgtcttttt cacgcagata ttggtcgagc acctgaccga tgccatcggg
      241 taacgagcgt acgcggttga tgccaaagcc gagcgaacgt ccgccgccga tgccgatgag
      301 ttggacaacg atctcgcgca ttcggtccac gggtgcgatg ggggaggcca tgcgcaaaat
      361 gtaggagatc aatctgccga tggcttcaga gactgcggcg atctcggagc cagccttggc
      421 cgtgttgaca aaggcctcga agggctgggt tccgccgttc tcgttgatgg tgatgaatgc
      481 cttgcccacg ggcgtttcga tgttgtaggt cttgccagtc aacgccttgg ggcgcggctt
      541 cttggtgcca tgccagatgg taggagctgc ctgttgtaca ggagccccct gcggggatga
      601 tacagttttc ggttcggcag aagcgggtgc tttcttatcc gcagtggctt tggtttccag
      661 aaccaccttg tcgcgtgaac cagtcacgta cacggtaatt cccttacagc cgagttccca
      721 ggccgtcatg taggccttgg ccacatcctc ttcggtggca ttctcgtgga agttgacggt
      781 cttcgaaagt gaattatcca cgaaggcttg cagcgcgcct tgcatgcgca catgttcttc
      841 ggcggtgata tcgcccgaaa ccacaaaggt atcgcgcacg gcttgcggaa tttcactgat
      901 gttctggcag gtgccttcgt tcatcacctg ctccacgatc tcctgtcgtt tctcttcacc
      961 gaggccgagt ttcttcaacg cctcatcgaa gcgcggactg gcgtaggtca gtttcaaatc
     1021 cttgccgttg tcgttcacgt ggcggatgta agccagcgcg aaaacgggtt cacagccata
     1081 gccttcgcag ccagccactg tggcgatggt tcccgtgggg gcgacagtgg tctgcgcagc
     1141 attgcggatg ccatgttgtt tgatgccgct gacgatcgca tcccacttaa cctcgggcct
     1201 gccccagtta tgctgatagt ccaccagcga tttgggcgcg tgccacttca tgtcatccat
     1261 gtcataaatg gaaccttcga tggcggggaa aggtccgcgc tcttccgcaa gttccacgct
     1321 ggtcttcatc gcatggaagc gcacgaactc catcacctgc gcgccgaatt cctggccttc
     1381 cttcgagcca tagcggactc ccgcatggta catcaggtca gcaagaccca tgatgcccaa
     1441 accgatgcgg cgtgcgcgat gcgcggcttc tttcaactgc ggcacagcgg gtacataggc
     1501 atttgcttcc accacatcat caaggaagcg tgtggccgtc acaaccgact ggcgcagggt
     1561 ttcccaatcc accgagccat cgggcccgca gtgttcattc aaattcacag aacccaaaca
     1621 gcagttctcg tacggtccaa gccattgttc gccgcaggga tttgttgcct caagcgggta
     1681 caagtgcgga acaggatttc cgcgattggc ggcatcgagg aacaacacgc caggttcacc
     1741 gttgtgatgc gcctgcttga cgatcttgtt atacagttcg cgggcattca ctttcctgta
     1801 agtgtgaatg gtgatgccag ccgcttccgc ctgttcgagc gtgccgctga atcccttttg
     1861 tttcatttcg ccgaggtcag ggaagcgcaa tggccattct gcatcatctt tcacggcttg
     1921 catgaaggca tcggtaatgc ccacagatat gttgaagttg gtgatggagt tttcgttgat
     1981 cttgcaggtg acaaaatctt ccacatcggg atggtccaca cgcagcaccg ccatgtttgc
     2041 accgcggcgg gttccacctt gggcgatctc accgaaggca tggtcataca cacgcaggaa
     2101 gcccacaggg cctgttgcct gcccagccga ggtctgcacg agcgcaccct tcgggcgcag
     2161 acgtgagaac gagaagccat ttccgccgcc cgtttgttgg atgagagccg catcgcgcag
     2221 ggtctggaag atacccgcac cgtcacggcc catgtcgtcg gtaatgggga gcacgaaaca
     2281 ggcggccaac tgtccaagcg gagttcccgc accggtaaaa gtgggagagt tcgggaaaaa
     2341 cttcttgctt gaaagcaggt ggtaatactc aacggtgcgc tgggctacat cagcgcccca
     2401 ttgttcttcg acctttgcca catggtgagc cacacgccag aacatctctt cgacattctc
     2461 agcgggtttg ccgtcatcgc cgcggcgtac gtaacgtttc atcaacacct ggcgggcgtt
     2521 gtctgtcaga gatgccttgg ggaggccctt tgccatggct ggcgtgggca gaagaccgtt
     2581 cttgactttg ggttcagcgg attttgcaac cattttttac tctcctgata aattattttc
     2641 gtagtgcaga ctttagtctg catttgtttt gatctgcatt tactgaaatt tgtactatta
     2701 agcctgcgct ccatagttgg atataaaaac gacaaagaca ttcccccctt ggaaggggag
     2761 cccttgtcgt tgttttaatc ttcgagcaac tggtcgatct ctgtgcgaat ggactgaagg
     2821 tcgtccagac ccaggtacac aatggcgtag cggatgtagg cgatttggtc cacttccttg
     2881 agggtggtca tcacaaggtc acccaccacg cgggaggaaa cttcagcctt gcccattttc
     2941 tggagttgtg tctccacctg gccgatcatg cgctcaatat ccgctgctga aacggggcgt
     3001 tttgcgcacg aaatgcggat tccccgcgcc aatttctcgc ggtcaaattc ctcacgtcca
     3061 ccatcctgtt tgatgatgag cggcgtggct aaaatggggc gttcgtaact gctgaagcgc
     3121 tgacggcatt tcaaacactc acggcggcgg cggattcccc cgtggctatc gtgggaggta
     3181 tcgagcacct tcgagtcatg gtgtttgcaa tacggacaac gcattaaatc tctccataaa
     3241 tagaacaaat gttagagcca aatatgggcg tttatgctct gcatccccgt atatatggct
     3301 aaagtgttcg gcattttagc accaacccca cccatatgca agaggacaac atggtgacag
     3361 tttccttaaa atattgcaca gccaaaaaat ttaacgttca taaatctgtt tcgacatgga
     3421 ttttcaaatt cttatcgtgg gcaacatcgt tttgaacgat atcaaaaaat cagatccaca
     3481 ccctccttgc gtagggtgag tgatatggat tgaaaagtta taatcgttca atgatgcgaa
     3541 tccgtttacg cctgcctgtc tttgccgcgt tgatcacggt gtttctcatc gcgcagcttg
     3601 tgcagccatc acaaacatgg atgttcctgc tgactgtgtt cagcggcata ttcatcagcg
     3661 cgtatctttg gacgcgttcg cttggcatga gtctgcaatt gcggcgtgag actcgtttgg
     3721 ggtggattca ggtcggaggt cagatcgaag aacgcatcac cttatccaat acatctgttt
     3781 tgcctgccgc atggattcaa ttcaaagatc attccacact tccagaattt catgcctcac
     3841 gctccaccag catcagcgca ggtttttttg atcaatggat cgtgaccgcc gacagcaacc
     3901 agcgtggact tttttatctg ggtgatgccg atatcgtgac gggcgatccc tttggaatat
     3961 ttgaagccac catccacgcc tcagagcgga cctcgatcct ggttctgccc caagtgctga
     4021 ctctccccga attctcgatc ctgccatctg gttcctttgg cgatggtcag ccgcggcgca
     4081 acgcacctga acaaaccatc catgcatcca ccgtgcgcga gtatgttcat ggcgacagtg
     4141 tccgcatgat ccattggccc accaccgcgc ggacaaataa agtcttcgtc cgtttgatgg
     4201 aaagcgcccc cgcaggggat tggtggattt tgctcgacct cgataaaaaa aatctcttcg
     4261 gcacaagttg ggattccatt gaagagcaaa gcgtaatgct ggccgcatcc cttgccagca
     4321 gaggattgcg cgaccgcaaa gccgtcggcc ttgtcaccaa cagcaaagac cccgcgtgga
     4381 tccatccgca aaaaggagaa ggccaacgat gggaaatttt gcaatcgttg gcgctggctc
     4441 agcccgctga actcaccctc acccaaaccc tcgagcgtat tcaacctttg cttgggcggc
     4501 ggcacagcct catcattgtg acggcttcgc ccaagcccga ctggttgaaa tcgctcccgc
     4561 cgttgatcaa aagcggaatt gtccccaccg ttttcttgtt cgatgccgct acatttggcg
     4621 gaaacgaatc tcctgcatcg gttgccgcca atctcgacgg gctgggaatc aaatatcacc
     4681 tcatctcgca tgggttgctg gaaccgccca aagtgcttcc ccgccagcag ggcggctgga
     4741 aatggcgttc cacccccaca ggtgagatcg tgccgatacg aagttgatcc atgcacatga
     4801 aatttttttc actgcgttcc ttcctgacct ggctcatttt gctccttgca atgggcgctc
     4861 tgaccgcagg cctgaaggat ggcattcttg atgtgcagga tgcggctttc ttttctgtgg
     4921 ccgcgttcac agtgacactc gcttttttga tcggcttcgg tacatcttcc ccgcgccgtg
     4981 catggagcat cgtactcgta agcggtgttt tatttgcggt catcgaaagc gcacggttga
     5041 tggagccgtt caaactcatc atccgtgcaa taccgccttt tgaacgtgac ttgctgcttt
     5101 ggttcatcga aagagattca cccgaagcca tctttccaga tgcgtccatt tttttgatcc
     5161 agttcaccga gttggtgcgg cgtgcagata ccttttttac gctgatcttc aatgccgcgc
     5221 ttgcccgccc aatggtgcgc gaattcattt gggacctgcc aatcctattt tgcgcggcct
     5281 gggcaggttg gaaaaccagc cgctccaacc aggccttgct ggcgcttgtg cctgcactgg
     5341 ttttgcaaac gtttattttg gaatacacag gcaaagacct gttcccgctt caacttggta
     5401 tctttaccct gctcatgctc atcggggtca atcacaactg gagcattgcc agtggcaatg
     5461 ggaaaaccgc gcgcgaaact tattcagcca ttttgatcct gtccattgca ttgacgatgg
     5521 ggtcggggtt tgtgccgtcc atttcgatca gtgaggcagc caaaaagctc acacgcaaag
     5581 atgatattgg caaagccctc ggtctggata aggaatctgt gcaggcttat gtcatctctg
     5641 gtctttctgg gttgccgcgt cagcatctca tcggcatgag tcctacgctg gctgaaacgg
     5701 tcatgttcac tgtgcaaacg ggcgaacttc ctcccaccga aaattcgatc atcgcgcagg
     5761 caattccgcg ccattattgg cgctggctga cctacgatgt gtacaacggt cagggctggt
     5821 ccacttctga agtgagcagc gtaacttatt cagcaggtga aacgatcttg agggagggcg
     5881 cgtcgtttca gaccttgcat caacaggtga aaaaatcttt cagcgaagat aaccgtctgt
     5941 attgggcggg gcagcttggt ggtgtggatc aacctgtcag cgtgaattgg cgcgatccgc
     6001 tttcagaggc ggatatgctt gggggcacca cgcaggccca aatttatagt gcggattcgc
     6061 tgctcgtcat cgtcagtgcg gatgaattgc gcgcgtctcc tgtcgtcccg ataactggtc
     6121 ctcaggaaaa atatttgtcc ctccccgtaa cagtgccgca gcgtgtgcat gcccttgccg
     6181 agtctttgac cgccgatttc aataacccgt acgataaagc caaagccatc gaaagatatg
     6241 tgcgtaccta tccatattca cttgatgtga ccccgcctgc accgaatcag gatgtggccg
     6301 attattttct cttcgatctg aaaaagggat attgcgatta ctacgccacg gccatggtgg
     6361 tgctggcccg ttcggtgggg ctgcctgcgc ggttggtggt tgggtatgcc aatggcattt
     6421 acaacgcgag aagcgcacag tacaccatcc gtgaggcaga tgcgcacagt tgggtggaaa
     6481 tttattttac aggggttggt tgggtggaat tcgagccgac agcaggccag ttggagctaa
     6541 acctgcctga cgatctttca gaggctgaga cgttgcctcc accagttttc cctgagcctg
     6601 tcagcagatt aagagatttc gacaagcagg gatttttccc tgagcgcaat cttctgcccc
     6661 cgattcttgc ggcggtgttg agcgtgttgt ttgttggaat atggtttttg tacgtgcagg
     6721 gcttgctgcg tcggcacgaa accattggct cgatctatga atatgtatat cagcacggca
     6781 ggaaaattta tgatgcctct cctgccaatg aaaccccgac ggtatttgcg caaaaactgg
     6841 cggcacggct aaaaagcggc aatgagtgga tcgctcctgc gcctgatgag gtccgctttc
     6901 tgacggcgct gtatttgcag gaaacataca gcgctcatcc cgtctcgcag gatgagcgcc
     6961 gtgaagcgat caaggtgtgg cgaaaattat tttggagatt gttgtacgcg caattcaaac
     7021 ggatataaca gaacgattta aggggaggtt gtggatgtgg gggtgaggat gttcccaacc
     7081 gtgatgttga ctgtgagcgt atcgccaaag aaaatgccat tggcatctgc catgcgccac
     7141 gaactttgaa ttagaccgtt ttgatcggtg gggacagcca gttgaatgga aatctcgcgc
     7201 tttgctccaa ctggaatagc ttcactgagg atgaaggttt cgccgcgcat tgggtcgccg
     7261 cctacgtttt gaaaggtgaa tccaggcggc caggcgcatc caccactgtt ttggaccagc
     7321 caggttttgg tgaaggtttc attgggtttc agatttgtgc catcggggat ggtcacatct
     7381 tcgatccaga tcaggtcata gcaggggtcg gctgcggcgg gtgtttcgag cgtggcggtg
     7441 aggacgactg gagttgcggt gaatttttcc gtcggcgctg gtgatgcagt tggaacagcg
     7501 gccagggtct cggtcagggc tgaagcgaag gtggagaccg cttctgtgcg cagtgtgtct
     7561 gtatccaatt ccacgggttg ggtcggtgtt gatttgttcg attcgcatga gaagagcaat
     7621 gcgcccagga tcagtgtcga aaaaaggatt aaacgtgttt tcatggtttg ccttccaaaa
     7681 gatactcctt taatggtgtt ggaagtatac aacaatcgtg attgaatttc agattgaatt
     7741 attgtacaat tgttctaata atccccattt gataatacag gtgcccatat gaatgcagtg
     7801 gaagtaaatc aactgcggcg aatctatcgc gcgcaaatcg gtgtctttaa tcgctccatc
     7861 aaggaagtgg tggctgtgga tgagatctct tttgatgtga agcaggggga gttgtttggt
     7921 ttgcttggcc caaacggtgc aggcaagaca accacagtga aaatgttgac cacgttgttg
     7981 atcccttcgc ttgggtccgc ttctgtggct ggttttgatg tggtgaaaca ggccaatgaa
     8041 gttcgcaagc ggatcgggtt tatcttcggc ggtgaacgcg gcctgtattg gcgtttgtcg
     8101 ggcgatgata acttgcgcta ttttgcaagc ctgtacggtg tggaccccga tgtttccaaa
     8161 aaacgaatcc catacttgat ggaactggtc ggtttgaagg ggcgcggcaa cgaacgggtg
     8221 ggcgggtact cacgaggcat gaagcagcgt ctgcacgtgg cgcggacctt gctgcacgac
     8281 cctgatatcc tgtttttgga tgagccgacc attggtctcg acccagttgg cgcacgcgag
     8341 ttccgtgcgg tgattttgga tctgcaagct caaaagaaga ccattttatt gaccacgcat
     8401 tacatgttcg aagccgatgc gctctcagac cgtattgcga tcatcaataa aggcagggtc
     8461 atcgcgctgg atacgccagg cggcttgaaa cagcacgtaa gcgatctttc ggtggtggaa
     8521 gtggagactt tcggtgcccc agattccacg cttgaaaaat tgcgaagcct gccttttatc
     8581 gattctcttt ctgtggaaga tcgcgaacag aagcaaatgc ttcttgtgca aacgaaacgc
     8641 ggcgccgaag ccgtgcccga tgtgatggcc gccctcgatg gacagcgtat cggcagggtg
     8701 atcgtccgtg agccgacttt ggaggatgcg tatgtgcgcc ttgttggagg tcaatcatga
     8761 actatgccaa ccttttccgc tattggcgca cggtgttgac cgtggctgaa atgtccatcc
     8821 gcacccagtt cagcgacagt tttattttgt ttgccatcgt ttttcaaccc atgatcattg
     8881 ccattctggc tttgttcatg ctgcgtgaaa aaggcggcga ttatgccatg ttcgtggtgg
     8941 tgggcagcgg cctgaccggg ctgtggagca gcctgctgtt tgtctcgggc aacagcatca
     9001 actcggagcg ctggactgga acgcttgaat cgcttgtggg cgtgcccaca cctttcgatg
     9061 tgatcgtatt tggcaagaac ctggccaatg tttttcagtc cctgctttca atggtcactt
     9121 cttatacgct ggcggctttc ctgtttgggt attcgctttc attcacgcag ccgttgctgt
     9181 tcatggtcac attgttgttt agcatgtttg ccttcatctg ctttgggctc accattgcac
     9241 cggtctttgt catgtatcgc agtgtgcagc aatggcagaa tgccatggaa ttccccgtgt
     9301 acattctggg cggattttta tttcccattg ccatgttgcc gggctgggca acccccatca
     9361 gctatttact tccgccttac tgggcggcgc aggccttgca cggcacttca accgccatga
     9421 tgcctttcga gcaaatcctg ttctgctggg ggatgatgat cgtatttagc gttgtggacc
     9481 tggtcattgc cagccagtta ttcaaggtca tgttgtataa agcacgcgta gatgcaaccc
     9541 tggacgtgga ttagacatga ataccattcg taaaaacctt tatcttttct ggcaggggac
     9601 gatcatttcc tatcgtgccc tgtttgcctg gctgcgtcca gttacctatg tggcttcaaa
     9661 ggtgttgatg ccgctggcgc aaatgttttt ctttaccttc cttgggtcat atgcgaccaa
     9721 cgggaagaac attgattttt atgtgatcgg gaatgccatt caaattgcat cggtgagcgg
     9781 tattttcgga gtgaccatga gcgtaggcgg tgaccgggat gcgggtacat tgccctacct
     9841 ttttggaact cccgccaatc gctttatgat cttcttcggg cgtgctttta tgcatgtgat
     9901 cgacggggcc ctgggagtgg tgatcgcttt gacctggggc gtcctactca tgggcctcga
     9961 tctttcgcgg accgatctgc ctgctctcgg tttaacgatc ttgatcacca cttttagcac
    10021 ctgcgggctt ggactattga tgggctgcct gagcctgctg actgccaatg tgatgttcgt
    10081 gaataatttt gtttattttt ccctgcttgt cttttctgga gccaatgttc caattgccac
    10141 cctgcctgag tggatgcagg ctgtctcaac cattctgccg ctcacacgtg ggatcgcttc
    10201 ggcacgtgcg cttgctgctg gagccagttt gagcgaggtt tctcctttgc ttctcagtga
    10261 agcaggcatc ggtctgtttt atggcgtgat cgggtatttt ctcttctctt ggtttgaaat
    10321 acaagccaag caaaaaggaa cgctggaaac cgtatgaagc ctgattcacc ccaaatgtaa
    10381 gaaatcagcc gagaattgtt cggaacgtcg cgaatatttt caaaagtcag aaagattaac
    10441 cacggagcca cggagacaca gagagaaaaa aataaaaact ccgtccacag gaggctccgc
    10501 actgcttcag tgtctccgtg gttcaaaatg gcttttcaaa gacaatcctt gaaaagtatg
    10561 caagtggtca aatgaattgc aagatatgga tgagttataa aaagccgacc cattttattg
    10621 ggtcggcttt tttcgtgtaa attcacggct aaagaattga aagattaaat tcgggatgtc
    10681 tcatgtttta atctttgaca agccattcct ttgcatcttt ttcattttca aaatacatga
    10741 tcggctggcc tgtgatgcgt gaaagcaggt cgcccaaggt gcgtttgacg cctgtgatgc
    10801 ccagcgtggc agtttttcgg acatgcgatt tcgtgttccg ggaggcttcg ttcaggagcg
    10861 gcatcaatgc gtttgtaact tccgtattcc tgaaatcaga aagcacaagg acggaatttt
    10921 caggttcgcc gatcacgacc tgctgcacct gggatagttc ttctgctaca gcttgtgtgt
    10981 tgaagaaaag attggaaaaa tcctggtata gaattttttt gccgttatgc tcgatccatt
    11041 tcgacttcat gatgtactcc tgtttctact tttttgcacg gtcttccctg taaagtgcga
    11101 ttcctgtgga aagggctcca aggaactccc aaaggcccac cccgaagaaa cgcatggggg
    11161 cgatgcctgt gataactgta gtagtgaaca cgatgaatgt caacgtgcgg aaggggactg
    11221 tccagcgata gaagatcttc acgtcattga aggcagccag aacataataa atgccgatgt
    11281 tcaaggatgc catggaggag gcggtcatga agacgatcgt gtaatcgctg cttgcacgcg
    11341 cagcgcggtc aataacctca aagccgagga tggagagcag aatttcgggt cggatcaggc
    11401 ccatcaatcc aagaatgaac gccaatgccc caaagataaa gatggtccag cctgaagcgt
    11461 cgcgaatttt catatgtaga ggtctcctgg tttattgcgt tgaatgatgt tttggaatgg
    11521 gtttttgccg cacctcttca aattatactt gtaaaccctt ctcatgataa aataaaaaaa
    11581 tctatggcga aacatctggt gctggtggct ggcaatattg gcgcgggaaa gacaaccctc
    11641 acagaacgaa ttggcgcccg ccttggctgg tggacgggat acgaatcggt caccgataac
    11701 ccgtacctct cggattttta tggggacatg catgcctggt cattccactt gcagatcttc
    11761 ttccttgggc atcgtgccga tcaatacctt gaagcctccc gcgatgcgcg ttccgccatt
    11821 ttagaccgca gtatttatga agatgctcat atctttgcgc gcgccctgca tcacatgggt
    11881 aacttgggcg aacgagatta tctggcttat cgccgcttgt tcgaggtcgt cgtcaatgga
    11941 ttgccgcgcc ccgacctgtt gatctatctt aaggcccccg tccccacatt gatggaccga
    12001 attcgcaggc gtgctagaaa tatggaaact ggcatcaccc cggattacct atccctgttg
    12061 gattcatttt acagcgaatg gctcagcgca tttgaccttt gtcccgtttt gaccattcaa
    12121 acagacgacc tcgattttgc ccatcacccc ggccatttgg atcttgtcgt tcagcggatt
    12181 caagacaaac tggcaggaaa agaagtcatg gacctcaccc aaaaataaca tgtcaaacac
    12241 cctgctctcg aaaatccccc tgttcgctgg ccttccccgc aaagagttgg accagatcct
    12301 tgccgccatg gaaaaaaagg aaatggccga ccgtgagatc cttttcaagg aaggcgatcc
    12361 cggcgagcat ttttatattg tcatgaaggg gaatttggaa gtgttgatgg cagcggggca
    12421 ggctgaagag cttttgttga atgtgatgag tgaaggggag cattttggtg aaatggggtt
    12481 gatcatgcct ggcgggcatc gcacggccac cgtgcgcgca cgcggagaat ccaccctgct
    12541 ttcgatgagc cgcgaaacat tcctgggcct tacccagaaa caccccgaac tttcaggctc
    12601 catggtgcgt gttctaagtc agcgtttgga tgccaccaat acccaaacct tccacgaact
    12661 caccgaaaag aatcgaaaac ttcaaaccgc gtacgatgaa ttaaaagccg cccaggcgca
    12721 gatcatcgaa aaagaacgcc tcgaacgtga acttcaagtt gctgcagaaa ttcaactcag
    12781 catcctgcca gacaaacttc ccgagccgcc caattttgat tttggtgcgc gcatcctgcc
    12841 tgcccgtcag gtggggggcg atttttacga cgtcttttat gttggcgaag ggctcatcgg
    12901 tgtcttgatc ggtgatgtgg ctgataaagg cgtaccttcc gcacttttca tgtctcgtgc
    12961 ccacgccttg gtaatggctg aggctgatac gggtctatcg gcaggcgaag tgatgcagct
    13021 ggttaaccgc catatcacgc gtttgcaaaa atccactcaa tttgtcacag tgttatacgg
    13081 cattcttaat ttgacgacca atgaatttag ttatgcccgt gccggacatg aacctccgct
    13141 cattttgcag cccgatggca ccgtggaacg attgccttac cgcccgggca tggcgcttgg
    13201 cctgtgggat agcatcctgc ttgatgaaca cgccatcacc ctgccgccag gctccacact
    13261 gctgttattt acagatggca tgaccgattg ccgtgacccc aacggggtgg catttggtct
    13321 tgataacatt aaatcggttc tcgaaaccct ccctgcggct tcagcgcaac aggtttgtga
    13381 ccacatgctc gatactctgg tgaaatatca gaatggctcc aaacaggatg atgatgtaac
    13441 ccttgtcgcc atccgtgcca ctggaaaata atcgatctcg catggtcgaa acataaaaac
    13501 gggtcacaag gaattttcct ttgtgacccg ttgtgattcc tgacagtaaa actttatcgc
    13561 agtgcctgtt ccagatcccc caacaaatct tcaacatttt cgatcccaac cgataatctc
    13621 accagcccgg ggtcaacctc cagcttcgaa ccctgtgtgg acaagtgcgt catcgccgct
    13681 ggcacttcga tcagtgattc gaccccgccg agcgattccg ccaatgtaaa tatctttgtg
    13741 gattccgcca ccttgacagc cgcatcccgc ccatctttca tcacaaacga gatcatgccc
    13801 ccgccattga gcatttggcg cttcgccacc tggacctgtg gatggctctc gtgaaatggg
    13861 tagatcagct gtttgacatt tttctgcttc tccaaaaacg ccacgattct ctccgcattc
    13921 tgcgcatgac gatccatgcg gatcggcagg gtcttgatcc cacgcagcac aaggaacgaa
    13981 tccatgggcc cctgaatccc gccgatggcg ttttgcaaat atgccagctt ctcgccgagt
    14041 tccttgtcct tgccaacgat ggcgccgccg accacatccg aatgcccgcc gagatatttt
    14101 gtcatcgagt gaacaaccaa gtccgcgccc atttcaagcg gacgctgtaa atacggggtt
    14161 gcgaaagtat tatccactac caataaaggc ttattaggat gatgctttac aacctctgcg
    14221 attgcgcgaa tatcactgac agccaacagc ggatttgtgg gagtctccag ccaaacgatc
    14281 ttcgtagacg gagtcaatgc ttcggcgaga ttctctggat cggtcgtgtc agcaaatgta
    14341 aagtcaagac cgaagcggcg cagtactttg tcaaataaac ggaaggtgcc gccgtacaca
    14401 tcgttgcctg cgaccacatg atcgccgctt cccagcatgc gcagaaccgt atcggtcgcc
    14461 gccatgcccg aggcgaaagc cagaccccac tgtccgcttt cgagttctgc aagacaatcc
    14521 tgcaaagcct tgcgcgtggg attcaatgtg cgcgaatatt catatccctg ccgcggcttg
    14581 ccgattccat cctgcatata cgtggatgtg agatagacgg gcgtcatcac cgcgccgttg
    14641 ttcgggtcgg gttcctgacc tgcgtgaatc gcgagagttt caaatttcat gtgtgctcct
    14701 taatgtgtag agcgagatac tatctcgctc tactgaatat ggtgatttat tttacccgtt
    14761 tcattttcaa acacatgcat ggatagcctg cattgtcacc ggcaaaacgc tcgatttcca
    14821 caaagccttc gctctcgtac aatgcaattg cttttttatt gccatcgagc gcgactgtcc
    14881 atgcgccttc gccgatctcg cggattccga ttttcaataa ttcacggcca ataccttttc
    14941 tgtagtgact cggatcaaca tacagccatg cgagataatc ctcgtccaca ccgacgaaac
    15001 ccacgactgt ttcatcgtca cacgcgacga atttgttggc gcgcttcaag tcgtccactt
    15061 cctcatcctg ctcgatggga ataaacccgc gcggatcgca cgagccgatc aattcatcag
    15121 gccgcgcacg gtcatggatc tggaaaatgg cctgccaatc tttcttttca taacttcgat
    15181 acgtgatcat ttttcctcgc gatttttatg aagttaattc ttttgtaatt ttgatcagat
    15241 ccat
//
LOCUS       DAOQLC010000103        15019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1446_length_15019_cov_27.903168, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000103 DAOQLC010000000
VERSION     DAOQLC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1446_length_15019_cov_27.903168"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..108)
                     /locus_tag="PL000_15905"
     tRNA            complement(36..108)
                     /locus_tag="PL000_15905"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Ile,seq:cat)
     gene            complement(188..259)
                     /locus_tag="PL000_15910"
     tRNA            complement(188..259)
                     /locus_tag="PL000_15910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Gly,seq:tcc)
     gene            426..3005
                     /gene="gyrA"
                     /locus_tag="PL000_15915"
     CDS             426..3005
                     /gene="gyrA"
                     /locus_tag="PL000_15915"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC90419.1"
                     /translation="MELGIVRKIDIDTEMQQSYLDYAMSVIVARALPDARDGLKPVQR
                     RILYAMYDMGIRADTAYKKSARIVGEVLGKYHPHGDSAVYEAMARMAQDFSMRTLLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSAALDILFDLNKETVDFVPNFDDTLEEPHVLPAA
                     IPNLLVNGATGIAVGMATSIPPHNLGEIVDAIVYMIERWEKLDDIDVEQLMEFVQGPD
                     FPTGGIIIQEKGEEGIESAYGSGRGRITVQAKAHIEEMERSKSRIIVTELPYTVNKSS
                     LIERIAELARDGHLEGLSDLRDESDRQGMRIVLEMTKNADPEAVLRELYKRTPMQTTF
                     SINLLALVDGEPRTLTLKQALRVYVEHRLEVIKRRAEFDLDKAKARAHILEGYLVALK
                     NLDELIGIIRNAPDVETARQKLMKRYKLTELQANAILDMQLRRLAALERKKIETEYKE
                     VTGVIKDLTTLLKSPKLMRGVVAEELLKVKAAYGDRRRTQIVNLKKGKASKMLTATDL
                     MPDQIVWVGVTADGVIARTHDDKQPKHSGNDAPRWLVKASTSDTLYLAAENGKAAAVA
                     MHVIPEAEKLSDGVPYQKVAPLGEDDVLSAMFALPARKSEFPPETCVISITRFGMVKK
                     SLVTELPGPSSQSFVLARVNEGDGLGWVGLTDGKKKDVLLVTASGMAIRFKEDDVRPM
                     GLVAAGVNGIKLDDNDEVVGAEILPAEGEIFLLASDGKAKRVDQKDFPSQGRYGKGVR
                     AWDLPKKITLVGAVSGKPNHMATIVLAKGAPKSARLDAAAVRKRASGRGDVIVEMKKD
                     EIITGLSVAWMVDRYVKLAKTVEEGEKKEEKAKKAAVKKTPAAKAKAKPAKASAKAKK
                     KKK"
     gene            complement(3074..3745)
                     /locus_tag="PL000_15920"
     CDS             complement(3074..3745)
                     /locus_tag="PL000_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90420.1"
                     /translation="MFINRLELPALDLIAQRDYYANVLELPVHLEGDSLLVKAGKTDL
                     IFTQAPPQWEGAYHFCFNIPENQFPYAKAWLSARIPLVKDDKGNDEFNSQSWNSTSLY
                     FKDAVGNILEFIARHRQQNSVNSPFNSGQILQVSEIGLASQDVISFAKELCAKLGVSV
                     YEQDFNDTFTPIGDEDGLFILPVEGRIWYPNTGIPAKMLPIKVDVEANGKLFHLEGVP
                     YRIKE"
     gene            complement(3754..4323)
                     /locus_tag="PL000_15925"
     CDS             complement(3754..4323)
                     /locus_tag="PL000_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNC90421.1"
                     /translation="MTREEALELVREFVKNEGLVRHMLSVEAAMRFYAEKFGEDVEAW
                     GLIGLLHDFDWEIHPTLEQHPQDGAPMLRERGVPEDIIQDILSHADHLKLPRDTMRRK
                     AICACDEVTGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGTDRSEMEHAAKEFG
                     IDIWEHVGNVITAMRKIAPELGLVGNLQQ"
     gene            complement(4375..5025)
                     /locus_tag="PL000_15930"
     CDS             complement(4375..5025)
                     /locus_tag="PL000_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNC90422.1"
                     /translation="MLKKIKILTWRIILTLSLIAILTLFLPRLITQLYAAPRIFTVDK
                     SPVEQVAIVFGAGLLRDGSAGPVLSDRVNTAVQLYHQGKADKLLMSGDNRFVEHNEPE
                     AMRQYALARGVPDEDIILDYAGQSTYDTCYRAREIFQVQSAILVTQSFHLPRALFLCN
                     WFGVESTGVEANNRYFRKISRLYWNFRELFATPQAAWEVLVAKPVPILGKPEPIFE"
     gene            complement(5033..6040)
                     /locus_tag="PL000_15935"
     CDS             complement(5033..6040)
                     /locus_tag="PL000_15935"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNC90423.1"
                     /translation="MKKLLLILTLTILACASPINLALEGATPTPTAETNSAPTATPQA
                     TPEPGTEKNPLILALGVSAHPSTQMVDAGKVIAAYLEGRTGYRIVIVVPPTENALVES
                     LQKGNTHIASLAPFSYLLARESDTVTAIMSSVHSGQVFYGAQFIANREKDFTSFYDAE
                     RNANTAEAATALKQFEGKKPCWSDNVSPSGYVVPLGVLNQAQVKIRDGAFIESQPSVV
                     RAVYADDICDFGATFIDARELPALEYDYADVLERVVIIWRVPPVIPYEIIAMSNSLPL
                     EMRRVIQRAFVDLMLTPEGKSAVQVVYGIDEMQIAEDSNYTDFLLYVKASGLDISGLL
                     E"
     gene            6308..6988
                     /locus_tag="PL000_15940"
     CDS             6308..6988
                     /locus_tag="PL000_15940"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HNC90424.1"
                     /translation="MKNTFNTIMSILILVVLAAGVYFIVQTVRESAQAAADAATAPFQ
                     QVNQANQALQTQVSQMMNPTPTIIPDPVTYINEIRALARLETIQYTVEKVITAEVGQG
                     TFGFLYGDKLLFVAHGIVIAGIDMEKLQPTDMRFDGQVLYVKLPPTEVFIATLDNDKS
                     YVYDRETGALTKGNVDLETIARQSAEDEIRKAALEDGILVQGQVNAEAYLLKFFTALG
                     YKSVIFEH"
     gene            complement(7023..7724)
                     /locus_tag="PL000_15945"
     CDS             complement(7023..7724)
                     /locus_tag="PL000_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90425.1"
                     /translation="MKRRVLLTGLVSALLAACNAATPQPTATPTPITVPSVQPTAPIP
                     PTTTPVTASLTVKDSPINCRLGPGTVYELINELDAGENALVVGRSEDFAWWYIRDPGN
                     PDGFCWVSANVTETQGIVEELPIIQPPVTTVTDVNLRVEPNRIVINCNQFPQTVFLEA
                     EITTNGPAFVIWRWEASTGAASNDSTLVFKESGPQIINDYYQFAEPNEYWIRLHILSP
                     NELTEQVNIPVNCTP"
     gene            complement(7910..9880)
                     /locus_tag="PL000_15950"
     CDS             complement(7910..9880)
                     /locus_tag="PL000_15950"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC90426.1"
                     /translation="MSILGLAGYGFLGLLLLIFLSGIRFIPNNRIGIIEKRFTFKGSV
                     KSGLIALNGEAGYQPRILRGGLHYLMPIQYVVHTMPLVTISQGKIGYVFARDGRQLDP
                     AQVLASNAEVTDFQDVEGFLKKGGQRGPQRRILREGTYAFNLVQFIVITEERIYTLPL
                     SKEEADTIRGMAQVIAERGGFRPVVIKDTDDSIGVVTVHDGPSLAQGEIIAPVVGDDP
                     DQSVNYHNKFQDADNFLRAGGQRGRQLQVLVEGTYYVNRLFGTVEMIPKTVIEVGNVG
                     VVVSYTGETGDDLSGKEYRHGELVNKGYRGVWSEPLLPGKYPFNTYAGKVISVPTTNI
                     ILKWIRTETGAHRYDENLTEVSLITKDAFEPSLPLSVVVHIDYQKAPLVIQRFGDIKK
                     LVEQTLDPMVSAYFKNVGQMRTLIQLLQDRSAIQQQASQEMREKFAHYNLALEEVLIG
                     TPSSPENDTQIETILMQLRSRQVAEEQVETYNQQEKAAVKERELREAQSRAQMQTKLT
                     EAELNINIQTDQGKAEYQRSIQQAQQIRALAEAEAEKVARIGIAQALATEEQVRAYGG
                     PQFQVTQQVLNRFAEAIQQSGVDVVPRVVVSGSGNGEGGNSSTSSSIMEGLLTMLLSE
                     RFGAMAKESQSPRSPEADALREQIRKDMEKKK"
     gene            10100..11899
                     /gene="lepA"
                     /locus_tag="PL000_15955"
     CDS             10100..11899
                     /gene="lepA"
                     /locus_tag="PL000_15955"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNC90427.1"
                     /translation="MTDHIRNFCIIAHVDHGKSTLADRLLQLTGAISERDMTEQALDS
                     MDLEREKGVTIKASAVRMFYNAKDGQRYELNLIDTPGHVDFGYEVSRALKACEGAILV
                     VDATQGIEAQTLANLYQALDADLTIVPVINKIDLPSARPDEVAEDVGSLLGIEPDSVL
                     RVSAKEGINVDQILEAIVQRVPSPKDADDDPVRALIFDAHYDSYKGVIAYIRVFEGKI
                     KATDVLRMFSTQTDLRPVEIGIFAPGMKPVENIGSGEVGYIATGFKTVHECRVGDTMT
                     LASAPASEPLPGYFTPKPMVFAGIYPVEADDYTTLRESLEKLQLNDASLSFLPETSQA
                     LGFGFRAGFLGLFHMEIIQERIEREYDLDVLFTAPSVEYEVLTIGDEVVKVDSPAELP
                     DPSKIVEVREPWMNIEIITPTDYYGPIMDLVTKRRGIFKKQEYPAPHRVQLDFEIPLS
                     EIIVDFFDHLKSRTKGYASLDYQFLEYRADKLQKLEILVNGDTVDALAAIVHEKDAYH
                     KGQRLITKLKDLIPRQLYDVAVQAASGGRIISRANVKATRKDVLAKCYGGDISRKKKL
                     LEKQKRGKKRLKMVGNVEIPQEAFMAVLKLDDE"
     gene            11935..12939
                     /locus_tag="PL000_15960"
     CDS             11935..12939
                     /locus_tag="PL000_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC90428.1"
                     /translation="MSQFLKDSKRWLPGALISVALIAAILYFVDINAMVNAIRSANYY
                     ILALAMSLSFVWMLGRAKVWQTLLRDKPAFRDVLFATGEGYLLNNFLPFRLGEIGRVF
                     LLSRKSGLQFGEVLPTVFIERSVDLAFSAAVFLAALPFVAGTAGSERIGIIVGIAVVI
                     GLLVMYFVARNNQWALGVFHKLSARWPSLQKFGGEFLESFFTGMGVLTDGWLFLRFLF
                     WMTLDWAIALLNYYLMLRAFFPQAEWIWGFFILGAAAFGGAIPSLPGAVGTFDGAIVG
                     ALTLLSGDQSTALAAALTARFYNYLNSGVIGGIGLMREGQTLTGVYQQIKDFRKKKEE
                     "
     gene            12936..13961
                     /locus_tag="PL000_15965"
     CDS             12936..13961
                     /locus_tag="PL000_15965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNC90429.1"
                     /translation="MKKNYLVTGGAGFIGSNYVHRLLQRGETVTIFDNLSRAGAPRNL
                     EWLKKEFGANAFEVVVGDVRDADRIAEAARSADVIVHLAGQVAVTTSVTNPRNDFEVN
                     ALGTFNVLEAARSSGRNPIFLYASTNKVYGGMDDVEVVEDATRWHYKDLTYGAPETQP
                     LDFHSPYGCSKGTGDQYVRDYFRIYDLPTVVLRQSCIYGPRQFGIEDQGWLAWMIIAA
                     VTGRQITIYGDGKQVRDVLHVEDLMDAYDAAVANIHVAKGQVYNMGGGVNNVLAIWAE
                     FGPMIENLLGKKVEVARGDWRPGDQRVFYADYRKAERELGWKPKISLEEGIERLFQWV
                     KENKDLF"
     gene            14148..14960
                     /locus_tag="PL000_15970"
     CDS             14148..14960
                     /locus_tag="PL000_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNC90430.1"
                     /translation="MQEYFSELAAQFVASIPNIVTALLIFILSLYFARLLSNFLKRVL
                     VRRKTDSGVTQLLTQTLQWTIIVFGAITALQRFFNVTAFLTGLGIIGFTVGFALQNVM
                     QNFVSGIILLMQQPFKVGEEIRVLDFDGVVLQINLRTTELHALDGRIVILPNADVISH
                     AIVNYTRANRRRVDLPLTIASTANPEKVRSLLLEVFKDTPGYVIAPAPEINFTSFNDT
                     SIGLTASFWVDTAVLKPEHAADAALVLVKNAFEKERVASPLPAQTVFLKSEQ"
BASE COUNT         3547 a         3746 c         4030 g         3696 t
ORIGIN      
        1 aattccatcc ctcgatgggg cgtgcgaaca atttgtgggc ccggcaggat tcgaacctgc
       61 gaccaagcga ttatgagtcg cctgcgctaa ccgctgcgcc acgggcccgt gttaagtacc
      121 aagtgttcca gtgtcatgtg ccaggcaaac tgacaccaaa gcacctgaca ctggaacact
      181 gatgaagagc gggagatggg attcgaaccc acgaatatca gcttggaagg ctgatgcctt
      241 accacttggc gactcccgca ttgcgagcgt gattttactg tgaggtgtga acccagtcaa
      301 gccacttgac gccttccatc tttccccgta agataagccc cgcaatagaa cataaattca
      361 ccacaaatag cgcaaagtta cgaaaaaaat ttctttgtat gccattgtgg ttatgaggaa
      421 ctcctatgga actcggtatt gtcagaaaaa tagacattga cactgaaatg cagcaatcgt
      481 atctcgatta cgccatgagc gtgatcgtgg cgcgcgccct gcccgatgcg cgagacggac
      541 ttaaacctgt acagcgccgc attctgtatg ccatgtacga catgggcatc cgcgctgata
      601 cggcttataa gaaatccgcc cgtatcgtgg gtgaagtgct tggtaaatat cacccgcatg
      661 gcgattcggc tgtatatgaa gccatggcac gcatggcgca ggatttttcc atgcgcacgc
      721 tacttgtcga tggtcagggt aacttcggtt ccgtggatgg cgacccgcct gctgcgatgc
      781 gttataccga ggcgcgcctt acttctgccg cactcgatat cctctttgac ctcaacaagg
      841 aaaccgttga cttcgttccg aacttcgacg ataccctcga agagccgcat gtattgcctg
      901 ctgcgatccc gaaccttttg gtgaatggcg caacgggtat tgcggtgggt atggctacat
      961 ccattccgcc gcacaacctc ggcgagatcg tagatgcgat cgtgtacatg atcgagcgtt
     1021 gggaaaaact ggatgacatc gacgttgaac agttgatgga atttgtgcaa ggccccgatt
     1081 tccccacggg cggcatcatc attcaggaaa aaggcgaaga gggcattgaa tccgcatatg
     1141 gctcggggcg cggacgcatc accgtgcagg ccaaggcaca catcgaagag atggaacgca
     1201 gcaagagccg catcatcgtc accgaattgc cgtacacggt caataaatcg agcttgatcg
     1261 agcgcattgc cgaactggca cgcgatggtc acctcgaagg cctctctgac ttgcgcgacg
     1321 aatccgaccg acaaggcatg cgcatcgtcc ttgagatgac gaaaaatgcc gacccggaag
     1381 ctgttctgcg cgaattgtac aaacgcaccc cgatgcagac cactttcagc attaacctgc
     1441 tggcattggt ggatggcgaa ccccgtacac tcactctcaa acaggcgctg cgtgtttatg
     1501 tcgagcaccg actcgaggtc ataaaaagac gcgctgaatt tgatctggat aaagccaaag
     1561 cccgcgccca tattcttgag ggatatctgg tcgcattaaa gaatctggat gaacttatcg
     1621 ggattatccg caacgcacca gacgttgaaa ctgcccgcca gaaattaatg aaacgctaca
     1681 agctgacaga gttacaggcc aatgccattc tcgatatgca attgcgccga ctggccgctc
     1741 ttgagcgcaa gaagatcgaa accgaataca aggaagtgac cggggtgata aaagacctga
     1801 ccacactctt gaaatcgccg aaactgatgc gcggtgtggt ggctgaagaa ctgttgaaag
     1861 tgaaagccgc ttatggtgat cgccgccgta cacagattgt caatttgaag aagggcaaag
     1921 cttccaagat gctcactgcc accgacctga tgcccgatca gatcgtatgg gttggtgtta
     1981 ccgccgatgg tgtcattgcc cgcacacatg atgataaaca gcccaagcat tctggtaatg
     2041 atgccccgcg ctggttggtg aaggcctcca cttctgatac gctttacctt gcagcagaaa
     2101 acggaaaggc cgctgcggtt gcaatgcatg tcattcctga agcggaaaaa ttgagcgacg
     2161 gtgtgccgta tcaaaaagtt gcgccgttgg gcgaagatga tgtactttcg gccatgtttg
     2221 ctttgcctgc acggaaatct gaattcccgc ctgaaacttg tgtcatttcc atcacgcgct
     2281 ttggcatggt taagaagagt ttggttaccg aactgcccgg gccttcttca cagtcgtttg
     2341 tccttgcaag ggtcaatgaa ggggatgggt tgggctgggt cggtttgacc gatggaaaga
     2401 aaaaggatgt tttgctggtc acagccagcg gcatggcgat ccgcttcaag gaagatgatg
     2461 tgcgcccgat ggggcttgtc gctgctggtg tgaacggaat caaactggat gacaacgatg
     2521 aagtggttgg tgcagagatc ctgcccgccg aaggggagat cttcctgctg gcgagcgatg
     2581 gcaaggccaa gcgtgtagac cagaaggatt tcccgtcaca aggccgctat ggcaagggcg
     2641 tccgcgcctg ggatttgccg aagaagatca cgctcgttgg cgcggtcagt ggtaaaccga
     2701 atcacatggc gaccattgtc cttgcgaagg gtgctcccaa atctgcacga ttggatgcgg
     2761 ctgccgttag aaagcgcgcc tctggccgcg gcgatgtcat cgttgaaatg aagaaggatg
     2821 agatcatcac cggcctgtct gtggcgtgga tggtggatcg ttatgtaaaa ctggccaaga
     2881 ctgtggaaga aggggagaag aaggaagaaa aagcaaagaa ggccgctgta aagaagacgc
     2941 ccgctgcaaa agcgaaggca aaaccagcga aggcttccgc aaaggcaaag aaaaagaaga
     3001 aataggaaag agaaaacccg catctgagac aaaacagatg cgggttttgt ttttgaaaag
     3061 ggtaagccaa tttttattct ttgattctgt aaggcacgcc ttccagatga aacaacttcc
     3121 cattcgcttc cacatccact ttgatgggca acatctttgc agggatgcct gtattgggat
     3181 accaaatgcg tccctcaact gggagaatga atagaccatc ttcgtcgccg atgggggtga
     3241 aggtgtcgtt gaaatcctgc tcgtacactg aaacacccag ctttgcgcat aattcttttg
     3301 caaatgagat gacatcctgc gaagccagcc caatttcgct gacttgcaga atttgtccgc
     3361 tgttgaatgg ggagttgacg ctgttctgct gtctgtgacg ggcgatgaac tcaaggatgt
     3421 ttcctactgc atccttgaaa tacagggatg tggaattcca tgactggctg ttgaattcat
     3481 cattgccttt gtcatctttt acgagcggaa tccttgctga gagccaggct tttgcgtacg
     3541 gaaactgatt ttcagggata ttgaaacaga agtggtatgc accttcccac tgtggcggtg
     3601 cctgggtgaa gatcagatct gttttcccag ctttcacaag caaagaatca ccctcaagat
     3661 gaacaggcag ttccaaaacg tttgcgtagt aatcgcgttg ggcgatcagg tccaaagcgg
     3721 gtagctcgag tctgttgata aacatatgac cagctactgc tgaagattcc cgaccaaacc
     3781 caattcaggt gcgatcttgc gcatggctgt gatgacatta ccgacatgct cccaaatatc
     3841 aatcccaaat tctttggcgg catgttccat ttcagagcgg tcggtgcctg cggcaaaggc
     3901 tttgtccttc catttctttt tgacggaact ggcttcaagg tcgtaaagcg aacgggacgg
     3961 gcgcaccaat gccacagcgg tgatgagacc tgttacttca tcacaggcac aaatggcttt
     4021 acggcgcatc gtgtcgcgtg gaagtttcaa atggtcggca tggctgagaa tatcttgaat
     4081 gatatcttca gggacgccgc gctcacgtaa catgggcgca ccatcctgtg ggtgctgttc
     4141 gagcgtgggg tggatctccc agtcaaagtc atgcagcagg ccgatcaagc cccaagcctc
     4201 cacatcctcg ccgaattttt cagcgtagaa acgcatggcg gcttccaccg aaagcatgtg
     4261 gcgcacaagg ccttcgtttt ttacaaactc acggacgagt tcaagggctt cttcacgagt
     4321 cattggggtt ctccttaata aggttgcgcg agacagcatc ccgcgttacg aaatttattc
     4381 aaagatgggt tctggtttgc cgagaatggg tactggtttt gcaaccaaca cctcccatgc
     4441 cgcctgcgga gtggcaaaca gttcgcggaa gttccaatac aaccgcgata ttttgcgaaa
     4501 gtatcggttg ttcgcttcca caccagttga ttcaacgccg aaccagttgc acaggaacaa
     4561 ggcacgcggc aggtgaaaac tttgcgttac aagaatggcc gattgcacct gaaatatttc
     4621 cctggcgcgg tagcaggtat cgtatgtgct ctggcctgca taatccagaa taatatcttc
     4681 gtcgggcacg ccgcgtgcaa gcgcgtattg gcgcatggcc tcgggttcgt tgtgttccac
     4741 aaagcgattg tcgccgctca ttaacaactt gtcggccttg ccttggtgat acaactgaac
     4801 tgccgtattg accctatcac ttaacaccgg gccagccgag ccgtcgcgca gcaagccagc
     4861 accaaacaca atcgccacct gctcaacagg agatttgtcc acggtgaaga tgcgtggggc
     4921 ggcgtaaagc tgggtaatga ggcggggcag aaaaagtgtg agaatggcga tcaggctgag
     4981 ggtcagaatg atgcgccatg tcagaatttt gatttttttc aacatggagg agttattcga
     5041 gcaatcccga tatatcaagc cccgaggctt ttacatagag cagaaaatcg gtgtagtttg
     5101 aatcttcggc gatctgcatt tcatcaatgc cgtacacaac ctgcaccgcc gattttccct
     5161 cgggggtgag catcaggtca acgaaggcac gctggatcac gcggcgcatt tcgagcggca
     5221 gcgaattgga catggcgatg atctcatatg gaatgacggg cggcacacgc cagatgatga
     5281 cgacgcgttc aagcacatcg gcataatcgt attcgagtgc gggcaattcg cgggcgtcaa
     5341 tgaacgttgc tccaaaatcg cagatgtcat cagcgtaaac agcgcgcacc acactgggct
     5401 gactttcgat gaaagccccg tctcttatct taacctgggc ctgattcagc actccgagcg
     5461 gcaccacata ccccgaaggt gaaacgttgt cgctccagca gggtttcttt ccctcgaatt
     5521 gtttcaaggc ggtggcggcc tcagccgtgt tggcgttgcg ttcggcatcg taaaaagagg
     5581 tgaaatcttt ttcgcggttg gcaatgaatt gtgcgccgta aaagacctgc cccgaatgta
     5641 cactggacat gatggctgta actgtgtcac tttcgcgcgc gagcaggtat gagaatggcg
     5701 ccagcgatgc gatgtgggta tttccctttt gaagcgattc aacgagggca ttttcagtgg
     5761 gcggcaccac aatgacaata cggtagcctg tgcgcccttc aagatacgca gcgatgacct
     5821 tgcccgcatc caccatttgg gtgctggggt gtgccgatac ccccaaagca aggatcagcg
     5881 gattcttttc agtgccaggc tcgggtgtgg cttgtggcgt tgcagtcggc gcggaattgg
     5941 tttcagcagt gggggtcggt gtggcacctt ccaaagccag attgatggga gaggcacacg
     6001 cgagaattgt gagggtcagg ataagcagga gttttttcat tttcaaaatt gtacatgcat
     6061 aattgcgcga tgttgctata ataactcacc ttgcatggat tctataacta ttcgggagtg
     6121 gctaaaagaa tgggctttga ctgccacaaa gtcacaaagg ctcgaaggca caaaggattc
     6181 ttggtggctt agtggcttgg cgtctttgtg attagcctat ggacttggtc actctcaact
     6241 atccgtgtgg aacaaaatcg ggcacaagtg cgtatatcat taataaggag attaagaaag
     6301 gacaatcatg aaaaatacat tcaatacaat catgtccatc ctcattttgg tcgtgctggc
     6361 tgctggtgtc tattttatcg tacaaactgt gcgtgagagt gcgcaggctg ccgccgatgc
     6421 tgccactgca ccatttcagc aggtgaatca ggcgaaccag gctttgcaga cgcaggtatc
     6481 gcagatgatg aaccccaccc ccaccatcat cccagacccg gtgacctaca tcaatgagat
     6541 ccgtgcgttg gcacgtttgg agacgatcca atacacggtg gagaaagtca ttaccgccga
     6601 ggtgggtcag ggaacctttg ggtttctgta tggagataaa ctgctgtttg tggcgcatgg
     6661 cattgtgatc gcaggcattg acatggaaaa gctccagcct accgatatgc gctttgatgg
     6721 acaggtgttg tatgtgaaat taccccccac cgaggttttt attgccacac ttgacaatga
     6781 taaatcctac gtgtacgacc gtgagaccgg cgccctgacc aagggcaacg tggacctcga
     6841 aaccattgcc cgtcaatcgg ctgaggatga gatccgcaag gccgcgttgg aagatggcat
     6901 tctggttcag ggtcaggtca atgccgaggc gtatttgctg aagtttttta cggcacttgg
     6961 gtataaatcg gtgatcttcg aacattaaga tggagtgcgg acgaaaagtc cgcactcttc
     7021 ttttatgggg tgcaatttac agggatgttc acctgttcgg tcaattcatt tgggctgaga
     7081 atgtgcaggc ggatccagta ttcgttcggt tctgcgaact ggtaataatc gtttatgatt
     7141 tgtggccccg attctttgaa taccaatgtc gagtcattgg aggcggctcc cgtgctggct
     7201 tcccatctcc aaataacaaa agcggggcca ttggtggtga tttcggcttc gagaaagacc
     7261 gtttgtggga actggttgca gttgatcacg atgcggttcg gttcgacccg cagattgaca
     7321 tcggttactg tagtgaccgg aggctgaatg atcggtaatt cctccacgat tccctgggtt
     7381 tcagtcacat tggccgaaac ccagcaaaac ccatccgggt tgccaggatc gcggatgtac
     7441 caccaggcaa aatcttcact tctgccgact acgagggcat tttctcccgc atcaagctcg
     7501 ttgattaact catatacggt ccctggccca agcctgcaat tgatggggga gtcttttacg
     7561 gtcaggcttg ccgtcacagg agttgtagtg ggcgggatgg gagcagtagg ctgcacactg
     7621 ggaacggtga ttggcgtagg cgtggcagta ggctgtggcg ttgcggcgtt acaggcagcc
     7681 agtaaagcag agacaagtcc tgtcagcagg acgcggcgtt tcatgtttcc tccttggaac
     7741 ataacaatct gcttgctgta acattaatta tccgtatttg gtgcgcagtt tcaagttaat
     7801 ttttgaaaat cagtacttga gagtgggtta tttttcagtg tttgtacgtg gaaaatgaaa
     7861 actcccccgc aaaaggtaca ttgcggggga gtttgtatat aacctgggcc tacttcttct
     7921 tttccatatc cttgcggatt tgttcacgca gggcgtctgc ttctggagag cgcggggatt
     7981 gggattcctt tgccatggct ccaaaacgtt cggagagtag catggtcagc agtccttcca
     8041 tgatgctgct ggaggtggaa ctgttgccgc cctcgccgtt tccgctgccg ctcacaacca
     8101 cacggggaac cacgtccaca ccagattgtt gaatggcttc cgcaaaacgg ttgagtacct
     8161 gctgggttac ctggaactgc ggcccgccat aagcacggac ctgttcttcg gtggcaagcg
     8221 cctgggcgat accgatgcga gcgacctttt cggcttctgc ttcggccaat gcgcggatct
     8281 gttgcgcctg ttgaatggag cgttgatatt cagccttacc ctggtcggtt tggatgttga
     8341 tgttcaactc cgcttccgtt agtttggtct gcatttgcgc acgggattgg gcttcgcgca
     8401 gttcacgttc cttcacggca gccttttcct gctgattgta ggtttcaacc tgttcttctg
     8461 ccacctggcg cgaacgtaac tgcatcagga tggtttcgat ctgggtgtca ttttctggtg
     8521 aagacggtgt accgatcagc acttcttcaa gtgcaagatt gtagtgcgca aatttctcac
     8581 gcatttcctg gctggcctgc tgttgaatgg cgctgcggtc ttgcaataat tggattaagg
     8641 tacgcatctg gccgacattc ttgaaatagg cagagaccat cgggtcgagg gtttgttcca
     8701 ccagtttctt gatgtcaccg aagcgctgga taacgagcgg ggctttctgg taatcaatgt
     8761 ggaccaccac tgaaagcggc agggacggct cgaaggcatc cttggtgatg agcgaaactt
     8821 cggtcaggtt ttcatcgtag cggtgtgcgc ccgtctccgt gcggatccac ttgagaatga
     8881 tgttggttgt cgggacagag ataacctttc ccgcatacgt gttgaatgga tacttgcccg
     8941 gcagaagagg ttcactccac acaccgcggt agcccttgtt aaccaattca ccgtggcgat
     9001 attccttgcc agaaagatca tcaccggttt cacctgtgta ggaaacgacc acgcccacat
     9061 taccgacctc aattacggtc ttggggatca tttccacggt gccaaacagg cgattgacgt
     9121 aataagtgcc ttccaccaat acctgcaact gacgaccgcg ctgaccgccg gcacgcagga
     9181 aattatccgc atcctggaat ttgttgtgat aattcacgga ttgatcggga tcgtcaccaa
     9241 ccactggggc gataatctca ccctgtgcga gtgatgggcc gtcatgtacg gtcacaacac
     9301 cgatcgaatc gtctgtgtcc ttgatgacca cagggcggaa accgccacgc tctgcaataa
     9361 cctgtgccat gccgcggatg gtatccgctt cttccttgct gagaggcagg gtgtagatac
     9421 gctcctcggt aatgacgatg aattgcacaa ggttgaacgc gtaggtacct tcgcgcaaaa
     9481 tgcggcgttg tggaccacgc tgtccaccct tcttcaagaa accttccaca tcctgaaaat
     9541 cagttacctc cgcattggat gccagcacct gtgcagggtc taactgcctg ccgtcacggg
     9601 caaatacata gccgatcttg ccttgggaaa tcgtcaccaa cggcatggta tggacaacat
     9661 actgaatggg catcaagtag tgcagaccac cacgcaggat gcgcggttga taccccgcct
     9721 ccccgttcaa agcgatcagg cccgatttca ccgacccctt gaatgtgaag cgtttttcga
     9781 tgataccgat gcggttattg ggaatgaaac ggatcccgga aagaaagatc agaagcaaaa
     9841 gacccaaaaa accataacct gccaaaccca aaattgacat tttttctcct ttgtgcgtcg
     9901 aattacgcac aatacataaa tacgaactgg ccccatcagg gttgcttgaa ttctaaaccc
     9961 tttcattttt gcgggtatcg ccctttgata ctacccagcg ggcaaaatgg gaaaaataaa
    10021 tcggttatac ttcgttcgct tacagggcag aaaccttccc tttgtagtaa atttaaaaaa
    10081 acgaaatttc gaaagcagca tgaccgacca tatccgtaac ttctgtatca tcgcccatgt
    10141 tgaccatggc aaatccaccc tcgcagaccg cctgcttcaa ttaacaggtg ccatctccga
    10201 gcgtgatatg accgaacaag ccctcgactc gatggatctc gaacgcgaaa aaggcgtcac
    10261 catcaaggcc tcggcggttc gcatgtttta caacgccaaa gacggccaac gctatgaact
    10321 taaccttatc gacacccccg gccatgtgga ttttggctat gaagtcagcc gcgcactcaa
    10381 agcctgcgaa ggtgccattc tggtggtgga tgcgactcag gggatcgaag cccaaacgct
    10441 ggcgaatcta tatcaggcgc tcgatgccga cctgacaatt gttcctgtta tcaacaaaat
    10501 tgacctcccc tcagcccgtc ccgatgaagt ggctgaagat gtaggctccc tgcttgggat
    10561 tgagccagat tcggttctgc gcgtttccgc caaagaaggc atcaatgtag accagatcct
    10621 cgaagccatt gtccaacgcg tgccttctcc caaagatgca gatgacgatc ctgtacgcgc
    10681 actgatcttt gatgcacatt atgattcgta taaaggggtc atcgcttata tccgtgtgtt
    10741 tgagggcaag atcaaggcga ctgatgtgct gcgcatgttc tccacccaaa ctgatttacg
    10801 ccctgttgag atcggtatct ttgcccccgg tatgaagcct gtggaaaaca ttggttccgg
    10861 cgaggttggt tacatcgcca ccggattcaa gaccgtgcat gaatgtcgtg tgggtgatac
    10921 gatgacgctt gcttctgcgc ccgcctcaga gcccttgcct gggtatttca ccccaaaacc
    10981 catggtgttt gcgggtatct accctgtgga agccgatgat tacaccaccc tgcgcgaatc
    11041 gctggaaaaa ctgcaactca acgatgcttc tttgagcttc ctgcctgaaa catcgcaggc
    11101 gcttggcttc gggttccgtg cgggtttcct tggtctcttt cacatggaga tcattcagga
    11161 acgcatcgaa cgtgaatacg atctggatgt cctctttacc gcgccatccg tggaatacga
    11221 agtgttgacc attggcgatg aagtggtgaa agtggattca cccgccgaat tgcccgaccc
    11281 cagcaaaatt gtcgaagtgc gcgaaccgtg gatgaatatt gagatcatca cacccaccga
    11341 ttactacggc cccatcatgg acctggtgac caagcgccgc ggcattttca aaaaacagga
    11401 atatcccgca cctcaccgtg tgcaacttga tttcgagatc ccgctttccg agatcattgt
    11461 ggatttcttc gaccatttga aatcgcgcac caaaggctat gcctcgctcg attatcaatt
    11521 cctcgaatac cgcgccgata aacttcaaaa attggagatc cttgtcaatg gcgatacagt
    11581 ggatgcgctt gctgccatcg tccatgaaaa ggatgcctat cacaagggcc agcgccttat
    11641 taccaaactc aaagacctga ttccgcgcca gttgtatgat gtggccgtgc aagccgcctc
    11701 cggtggacgt atcatcagcc gggccaacgt caaagccacg cgtaaagacg tgctggcaaa
    11761 gtgttatggc ggcgatatct cacgcaagaa gaaactgctt gagaaacaaa aacgaggcaa
    11821 gaaacgtctc aagatggttg gtaacgtgga gattccgcag gaagccttca tggccgtgtt
    11881 gaaactggat gatgaatagc ctactggata gttgccagta aatcgtaaat cgaaatgtcc
    11941 caattcctca aagattccaa acgctggctg cctggtgccc tcatcagtgt ggcgttgatc
    12001 gctgcgattc tgtacttcgt ggatatcaat gcaatggtga acgccattcg ttcggccaat
    12061 tactacatcc tggcactggc catgtctctc tcttttgtat ggatgcttgg ccgtgcaaag
    12121 gtttggcaga cgcttttgcg tgataaaccc gcatttcgcg acgttctttt tgcaaccggg
    12181 gaaggctacc tgctcaataa ttttctcccc ttccgcctcg gtgagatcgg acgtgtcttt
    12241 ttgctcagcc gcaaatcggg tttgcagttt ggcgaagtgc tccccacggt tttcatcgaa
    12301 cgctccgtag acctggcatt ttccgcggct gtatttttag ccgcgttgcc ctttgtggct
    12361 ggcactgcag gctctgaacg catcggtatt atcgttggta ttgcagtggt cattggtttg
    12421 ttggtgatgt attttgtggc tcgcaacaac caatgggcgt tgggtgtctt tcacaaattg
    12481 agcgcgcgct ggccgtcttt gcagaaattc ggcggggaat tccttgaatc atttttcacc
    12541 ggcatgggtg tgttgacgga cggctggttg tttctgcgtt ttctattttg gatgaccctc
    12601 gactgggcga tcgccttgct gaattattac ctgatgctgc gggcgttctt tccgcaggca
    12661 gaatggatct ggggattttt catccttggt gcggcggcgt ttggcggcgc gatcccttct
    12721 ctgcctgggg cagtgggtac atttgacggc gccatcgttg gggcattgac cctgctctcc
    12781 ggcgaccaat ccactgcatt ggcggcggca ttgactgcgc gtttttacaa ctacctgaac
    12841 agtggtgtga tcggaggcat tggcctcatg cgcgaaggtc aaacattgac aggcgtttat
    12901 caacagatca aagatttcag gaagaaaaaa gaagaatgaa aaagaactat cttgttaccg
    12961 gcggtgcggg gtttatcggc tcgaattatg ttcaccgatt gttgcagcgc ggcgaaaccg
    13021 tcaccatctt cgataatctt tcgcgtgcag gcgcgccgcg caacctggaa tggttgaaaa
    13081 aagaatttgg tgcgaatgcc tttgaagtgg ttgtagggga tgtgcgcgat gccgatagaa
    13141 tcgctgaagc agcccgcagc gcggatgtga tcgtacatct ggccgggcag gtggcagtca
    13201 ccacctccgt taccaatccg cgtaatgatt ttgaagtcaa tgcattgggc accttcaatg
    13261 tattggaagc cgcacgttca tcgggcagga acccgatctt tttgtacgcc tccaccaaca
    13321 aagtgtatgg cggcatggac gatgtggaag tggtggagga tgccacgcgt tggcactaca
    13381 aagatttaac ctacggtgcg cccgaaaccc agccattgga tttccattcg ccttacggat
    13441 gctcaaaagg cacaggcgat caatacgtgc gcgactattt ccgcatctat gacttgccga
    13501 cagttgtcct gcgacaaagc tgcatttacg gcccgcggca gtttggcatt gaagaccagg
    13561 gctggctcgc gtggatgatc atcgccgccg tgacaggacg ccagatcacc atctacgggg
    13621 atggcaaaca ggtgcgcgat gttttgcatg tggaagattt gatggatgcc tatgatgcag
    13681 cggttgccaa tatccacgtt gcgaagggac aggtctacaa catgggcggc ggtgtgaaca
    13741 acgtgctggc gatctgggcc gaatttggcc cgatgatcga gaacctgctt gggaaaaagg
    13801 tcgaagtggc tcgcggcgac tggcgccccg gtgaccaacg tgttttttat gccgattatc
    13861 gcaaagcgga gcgcgagttg ggttggaagc cgaagatcag ccttgaggaa ggcattgagc
    13921 gtttgttcca atgggtgaag gaaaacaagg acctgttcta ggcagccatc tgaaagagtt
    13981 tgtaaagttt tccgctgctc aagccgccga gaacatattt tgaaaaatgt ttttgaaact
    14041 tccgaaaggc tcactttcgg aagttttatt ttcatcgcgt tataatctgg ctctctggaa
    14101 ttgatatcag catctgcgta accggcagat agcccattgg agtttttatg caggaatatt
    14161 tttcggaact tgccgctcag ttcgtcgctt ccattccaaa tattgttacc gcgttgttga
    14221 tctttatcct cagcctgtat tttgccaggc tgttgagtaa ttttctgaaa cgggtgcttg
    14281 tgcgccgaaa gaccgactct ggcgtgacac agcttctcac ccaaaccctg caatggacga
    14341 tcattgtgtt tggtgcgatc actgcgttgc agcgattttt caatgtcact gcttttttga
    14401 ccgggttggg gatcatcggt tttacggttg gctttgccct tcaaaatgtg atgcagaatt
    14461 ttgtgtcggg catcattctt ttgatgcagc agccgttcaa agtgggcgag gagattcgcg
    14521 tgctggattt tgacggtgtg gttttgcaga tcaacctgcg caccaccgaa ttgcatgcgc
    14581 ttgatggccg catcgtcatc ctgcccaatg cagatgtgat ttctcatgcc attgtcaatt
    14641 acacccgcgc caaccgccgc cgcgtggacc tgccgctcac gatcgccagt actgccaacc
    14701 cggaaaaggt gcgttccctg ttgttggaag tgttcaagga tacgccgggc tatgtcattg
    14761 cgcctgcacc ggagatcaat tttacgagtt tcaacgatac ctccattggg ttgaccgctt
    14821 cattttgggt ggacacagca gtgttgaaac ccgaacatgc cgccgatgcc gccctggtat
    14881 tggtgaagaa tgcctttgaa aaagaaagag tcgcaagccc gctgcctgcc caaacggttt
    14941 ttctaaaatc ggagcagtag cagttatttt gagctgattc gctggagcag cgtgattcac
    15001 cccaaacgta aggaatcag
//
LOCUS       DAOQLC010000104        14848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1470_length_14848_cov_23.065977, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000104 DAOQLC010000000
VERSION     DAOQLC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1470_length_14848_cov_23.065977"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1146)
                     /locus_tag="PL000_15975"
     CDS             complement(58..1146)
                     /locus_tag="PL000_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90431.1"
                     /translation="MKKHLMVFLTFILMACAAPAAEVSPTEGAAPLATVTAIPPTETA
                     EPQTFFKVFGDAPIVSKGESGTWDDRYTDPGAVVFHDGIFHMFRNGFRGFPAESQVGY
                     VTSVDGYTWEKQGSDPVFLTKDVPYAQVAMFVSSALVEDDGTWVLYFYTLDSVNFPFS
                     GVIGRATAASPNGPWIPDAEPVLMPGSAREWDSRNVAAPHVIKTADGYVMYYSGTDST
                     GKVQVGMAASSDGVQWAKYNDPATTEIQYQESDPVLLLGEKSSWESDQVHQARVFPDG
                     DGWVMIYRGSNKNQASMGLGIATSRDGIHWAKSALNPVLLPTEIKGAYQFWFHNALFV
                     NGTYFVFIEGDINQRTQIYLATREGEMP"
     gene            complement(1151..2737)
                     /locus_tag="PL000_15980"
     CDS             complement(1151..2737)
                     /locus_tag="PL000_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNC90432.1"
                     /translation="MRRYIVTMRIISLTLILLCLLTACTSPTAIPTPIPSPTTTPPPL
                     TGEWIGTAAKPDGSIASVIVNFDEDIPELNIEPMTRKWKLELKQEGGQLRFLAEGRTR
                     DPFERIEFSGRAENGLISGEITWDGHTSQTHFLQLAAVTDEILASYEGVYRFDSGRTL
                     SIIVSPEFSSSGLEYFGKSLMMTDFENGNLRSLYAVDDSTFLVGVLRVVGAPFDGRIE
                     FMKDGQGNIEGLMWWQTPNDILSSPVPSEFAARVPFVMDDGVSYFSADGTKLVGRLTM
                     PSSAGPHAAFVMPHGSEAGTRDNFGAKLTAHYFLSRGIATLNYDKRGVGDSEGIYHES
                     ADSTNLKNLAADANAGVEYLLTRSEIDAQRIGLIGGSQAGWLIPIAASESEHVSFFVI
                     LSGPVASTLQEDIFSAYTDDGESSTSYDDAEITRKLRSLGFGGFNPIPYLAELTQPGL
                     WLWGSVDKSVPVTFSRENLQVLIDGGKNNFEYQVLAGGDHGLSITPNGLFSEMPYSPG
                     LALYGPLTEWLEKNNISAKE"
     gene            complement(2775..5471)
                     /locus_tag="PL000_15985"
     CDS             complement(2775..5471)
                     /locus_tag="PL000_15985"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC90433.1"
                     /translation="MTVPAMIPTKINIPRPRMDVVVRPRIFSILDEGWAAPTALTLIS
                     APAGSGKTTLLVNWLQNKKHKAAWFSLGDEDDLFPRFVSYVIAALQSLDPVLGRSAAK
                     AFTSGDGQTPQSEAVINSLLRDCAVLEHPVALVLEDYHFIKSAEIHRLVTEIAEYLQP
                     YLRLFITTRNDPPLPLAKWRVRHQLVEIRSSDLQFTAQETGEFLRRVMKVDLPAAELT
                     NLQQQTEGWAAGLQLAALSLRSRKQQGIAWSWGDGNRDLSDYLMAEVLGQLPPEKQDF
                     LMQTALVTRFSAALCNTLTGGNNAQALLDELDSENLFLVSLDDSREWFRFHHLFADFL
                     QKQALIRYPAEAIRGLHQRAARWLAGQGYVTEAIEHALAAQDYEFAAALIGPQSQEWM
                     RRGEVATILEKMKQLPDEIVSKNAGLCIWYGWVYALGDSPQLAERWSDRAEAVLSPDL
                     KTVAADPEQFGPGIRNSHAQILAIRATAARHRHDFQTAVQLAEQALRIVPKGNTHLQM
                     IISAGLSTSQIEVGDFAQADSVTSATRQLAYQTGHPFIEFSMLINEAVLAMARGQLYK
                     AHQVAEDALHLADAESMQHLAFIPHGYLGEVNFLWNKLNEARQHILQGNHNLNFKEYP
                     TSSVRAYLNLAMLHQAEGEFAQALDALEKARRFAQKHQVADLFERAKGLQALYQFSAG
                     KTEAVERWAKSSGWDSFDPVQSKLLFNEESFYAACRYRIDLAKPTEYQKVEALLEWCL
                     GEAEAQQRVGVILKVRLLQVCLYQAQGESDAALAALVQALTLAEPEKFIRPFLDAGEL
                     IESLLPRVPHSHSARAFAQTILTNFHTQKPQPMPVLLEPLNEQELGILRLMAQGFSNP
                     EIAQKRALAVSTVRWYVKHIHRKLGVHNRTQAAARAREMDLL"
     gene            complement(5569..5949)
                     /locus_tag="PL000_15990"
     CDS             complement(5569..5949)
                     /locus_tag="PL000_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90434.1"
                     /translation="MDPRLEQDALIEDALKSQPLAPMPRSITANVMSRIQAVPSQQRP
                     ALVTWNDFVLSLFIALSIGAVLFALQSLPPVAVVKLQIQWILLYQDFIVNARWLLPAL
                     SFGLAAFFAALTIPTLVQMVSNRQ"
     gene            complement(5934..6500)
                     /locus_tag="PL000_15995"
     CDS             complement(5934..6500)
                     /locus_tag="PL000_15995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC90435.1"
                     /translation="MVISEQILVQKAQKGDVQAFEALVNEHQQYVYNLALRVVKDENE
                     ALDLAQETFVRAWTALPNFKGQSQFRTWLYRIVTNLCYNRLPNLRRSLNDLGDDMMED
                     IPEPYLDTPAQIFEISETRKHLQDAIQSLDSNYQLLITLRYQNELSYEEIASTLNLPL
                     GTVKTGIFRAKEQLRKSLAQLEESWIPA"
     gene            6705..8012
                     /locus_tag="PL000_16000"
     CDS             6705..8012
                     /locus_tag="PL000_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNC90436.1"
                     /translation="MKRPIVITLLIVALVLVCAGIGSVIYFANGFSVNNPFDKLNISS
                     VLEESKTLKVDTTKPIKLKVLDDAGTVTITGADVETVQVRIVKTAYDSSQARADAEVK
                     NIKYTIEQNGDTITLKYELPKSVNFSNHINTVDFIVTVPTEVVVSVNNSSGEVSVADT
                     KGNVDIKNSFGDVTIENIEGALTVHTNSGKVTATSIVAGTENIDLSSDFGEIILKKAG
                     GTDISLDSNSGNITLSEVRVTGDLTANTDFGNTEFENGSADALHVETNSGKVSLTKLK
                     ISKLLFVKNDFGDIELDQAMADSYDMHTNSGDITVDGAKGKLKADTDFGNVTISNAQS
                     VTLTVETNSGTIEFSGSLGVGPHMVKSDFGTIDISLPADVKLTVDLKTDFGKIKSELP
                     VTVILTDGARSDGDQIAGDINGGGEQLTVQANSGNITIRVSSQ"
     gene            8091..8426
                     /locus_tag="PL000_16005"
     CDS             8091..8426
                     /locus_tag="PL000_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90437.1"
                     /translation="MNDIIPCLGVVGSLTIIFGFFAFLRYINYKETIALAEKGLVKSE
                     SKPNKILLRWGIVITALGFALSLGLYTLGFASADNYPLHLGPWMLGGFVPLFLGLGLV
                     LLHYLTEKE"
     gene            complement(8431..9486)
                     /gene="hypE"
                     /locus_tag="PL000_16010"
     CDS             complement(8431..9486)
                     /gene="hypE"
                     /locus_tag="PL000_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNC90438.1"
                     /translation="MENEIDFEGYTCPVPVQPRDTVVLGHGSGGKLSHDLINRLFLPD
                     LGKAAPRALDDAAVLNINGQRLALTTDSHVVSPLFFPGGDIGRLAVCGTVNDLAMVGA
                     RPIALTCGFVVEEGLSFEILQQVVRSMRESAAEAGVYIAAGDTKVVQKGGADKLFINT
                     SGVGVVETGVNISGANAQIGDVLIMSGTMGDHGVAVLSARENLQFETDLQSDVAPLNH
                     LVAAMMGAGEIHVLRDPTRGGVATTLNEIASQSKVGIELLETALPVKPQVRAACEMLG
                     FDPLNIANEGKLLAIVKAADAESILSAMRKTKYGEEACIIGKVIETKSPQVRLRTAIG
                     GTRLIDMLPGEMLPRIC"
     gene            complement(9502..10587)
                     /gene="hypD"
                     /locus_tag="PL000_16015"
     CDS             complement(9502..10587)
                     /gene="hypD"
                     /locus_tag="PL000_16015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNC90439.1"
                     /translation="MKYIDEYRDPAKVRGLLAEIGETVTQPWVLMEICGGQTHAFLHH
                     GLDEMLPGGIELVHGPGCPVCVTPLEQIDKALAIAARPEVIFTSYGDMLRVPGSSVDL
                     FNVRAKGGNVKVVYSPLEALKLAQENPEKEIVFFAIGFETTAPPNAMAVLQAKQKGIK
                     NFSVLVSHVCVPPAMEAILGSKHNRVQAFLAAGHVCAVMGYHEYPAIAEKYKVPIVVT
                     GFEPVDLLRGVLMAVKQLESGKAEVENAYERAVTFEGNQAAQQVVQTVFQPADRKWRG
                     IGLIPQSGWGLRPDFAEFDAEARFSVGEIITQESPLCIAGEILQGMKKPPQCPAYGKQ
                     CTPSSPLGAPMVSAEGACAAYYRYHRE"
     gene            complement(10584..10832)
                     /locus_tag="PL000_16020"
     CDS             complement(10584..10832)
                     /locus_tag="PL000_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNC90440.1"
                     /translation="MCLGIPGKLQKIYDEGSLKMGKVDFGGIVKEACLAYVPEVQVGQ
                     YVLIHVGFAISVLDEEEAQARLQMIHEIDALGEELGSV"
     gene            complement(10891..13185)
                     /gene="hypF"
                     /locus_tag="PL000_16025"
     CDS             complement(10891..13185)
                     /gene="hypF"
                     /locus_tag="PL000_16025"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNC90441.1"
                     /translation="MRSIRVHVTGIVQGVGFRPFVYGLAVRLGLRGWVLNTSAGVDIH
                     VEGAEVQVALFIEKLSSELPPLATLDSVTVKEAEVENHSVFTIQPSNHIEGAFQPVSP
                     DVALCADCERELFDPQNRRYLYPFINCTNCGPRFTIIKDLPYDRPATTMADFPLCEDC
                     RAEYENPTDRRFHAQPVACPTCGPFVQLRSLSTGHVSNIELRLSAVLKTRQFLREGRV
                     VAIKGLGGFHLACDAMNERAVNELRQRKGRAGKPFALMFANLDTVMQYCEVNEEEAKL
                     LTGHEKPIVLLKLKAHTSLAANVAPHMDTLGAMLPYTPLHHLILNQTDEKLNAQSVPP
                     VLVMTSGNLSEEPISITNEDAFEKLSPLADAFLLHNRDIHVRCDDSVVRADGRGITFL
                     RRARGYAPYPVLLPFESRPTLAVGGELKNTFCLTRDRYAFLSQHIGDMENVEVYESFE
                     QNVERLSHLFRVQPQVIAHDMHPNYFTTQWAQKMEGRRVPVQHHHAHIAACMADNRLT
                     DRRVIGLSFDGTGYGTDGAIWGGEVLLASYADFERVAHLQYLPLPGSDSATRHPWRIA
                     VGYAEALGLEINDLPFMQGLDATAVEIVRQQVRKDLNTPHTSSMGRLFDAVASLGGVR
                     SDVSYEAQAAIELEVLSKRFINGAEAYPYQIRNGIVLLGELLQDVIADIRSKQPVGLI
                     GARFHQTLCALAVEVCRNTRNETGINEVALSGGVWQNQTLLNLTREKLADERFVVFSH
                     RQTPANDGGLALGQAVIANYSSER"
     gene            complement(13202..13858)
                     /gene="hypB"
                     /locus_tag="PL000_16030"
     CDS             complement(13202..13858)
                     /gene="hypB"
                     /locus_tag="PL000_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNC90442.1"
                     /translation="MTTRIPVVEKILSANDRMAERNRNFLDQAGVFAINIMASPGAGK
                     TSTILKTLETLRGRLKLGVVEGDLASSIDAEKAAAAGLPAVQINTGGECHLDAAMLSD
                     ALPTLPLSEIDLLLVENVGNLVCPANFQIGTHLNVLIASIPEGDDKPYKYPGMYRDVQ
                     ALIINKIDLLPYVPFNMEYFRKGVEVLNPGLVTFAVSAKTGEGMEAWTDWLVQNIKSK
                     "
     gene            complement(13878..14258)
                     /gene="hypA"
                     /locus_tag="PL000_16035"
     CDS             complement(13878..14258)
                     /gene="hypA"
                     /locus_tag="PL000_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNC90443.1"
                     /translation="MKIPFLAIIKVVAMHELAVTESILDISLRHAKEAGASRVTDINL
                     VIGQFASIVDDSVSFYWNIVAEGTMAQGARLHFERIPAEMACSACGHTFHPDDETFEC
                     PSCASMQVRITQGEEFRVDSIDVE"
     gene            14514..>14848
                     /locus_tag="PL000_16040"
     CDS             14514..>14848
                     /locus_tag="PL000_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HNC90444.1"
                     /translation="MEIPRHWRLKQQRYGLVGEVCPHCDYKIFPPRDVCPNCGDEAKE
                     LFTFSGKGQVYSYTTVYEAPAGYDANAPYTVAIVKLDEGPMVTAQLTDVDNSEVQIGM
                     PVEMVTRKMR"
BASE COUNT         3661 a         4006 c         3703 g         3478 t
ORIGIN      
        1 gatggacggc agacagtgtt tctttttgaa aagccactat ctaccgtcca ttttcattta
       61 tggcatttcg ccttcacgcg tcgctaaata aatttgcgtc cgttgattaa tatcgccttc
      121 gataaaaaca aaataggtgc cattcacaaa cagtgcgttg tggaaccaga attgatatgc
      181 gcctttgatc tcggtgggca aaaggacggg attaagcgca gacttcgccc agtggattcc
      241 atccctgctg gtggcgatgc ccaaccccat actggcttga ttcttattcg acccgcggta
      301 gatcatcacc cacccatctc catctggaaa gacacgcgcc tgatgcacct ggtctgattc
      361 ccagctgctt ttttcaccaa gcagcaaaac ggggtcgctt tcttgatatt ggatctctgt
      421 cgtggcaggg tcattgtatt tcgcccattg aactccatca ctcgaagcag ccatgccaac
      481 ctgcacctta cccgtgctgt cagttccact gtaatacatc acatacccat ccgctgtctt
      541 gatgacgtgc ggcgcggcaa catttctcga atcccattca cgcgccgacc ctggcatgag
      601 cacaggctcg gcatcgggta tccaggggcc gttggggctg gccgccgttg cccgtccgat
      661 caccccacta aagggaaagt tcaccgaatc aagcgtgtaa aaatacaaca cccaagtccc
      721 atcgtcttcc accagcgcgc tggaaacaaa catcgccacc tgggcatagg gaacatcctt
      781 tgtcagaaaa acggggtcac ttccctgctt ctcccaagtg tacccatcca ccgaagtgac
      841 gtagcccacc tgtgattcag cggggaatcc gcggaagcca ttgcgaaaca tgtgaaaaat
      901 tccgtcatgg aagaccacag cgccagggtc ggtgtagcgg tcatcccagg tacctgactc
      961 acctttggaa acaatgggcg catcgccaaa aactttgaaa aatgtctgcg gctctgcagt
     1021 ttcagtgggc ggaattgcag tgaccgttgc aagcggcgcg gcgccttcgg tagggctcac
     1081 ttccgcagca ggggcggcgc aggccatcaa aataaatgtc agaaaaacca taaggtgttt
     1141 tttcattgaa ctattccttt gcagagatat tatttttttc aagccattcc gtcaaaggac
     1201 catacaacgc cagccctggc gaataaggca tttccgagaa gagaccattg ggggtgatgc
     1261 tcagaccatg atctccacca gcaaggactt gatattcaaa attgtttttg ccgccatcga
     1321 tcagaacctg taaattttcc ctgctgaaag tgacaggcac gcttttatcc acactgcccc
     1381 atagccacag gccgggttgt gtcaattcag caaggtatgg gatcgggtta aacccgccga
     1441 agcccaaact gcgcagttta cgggtgatct ctgcatcgtc ataactcgtt gaagattcac
     1501 cgtcatcggt gtaggcgcta aagatatctt cctgaagtgt ggatgccacc gggcctgaaa
     1561 gaattacaaa gaaagaaaca tgttcagact cagatgcggc aatcgggatc aaccaccctg
     1621 cctgacttcc gccgatcaaa ccgatgcgct gcgcatctat ttctgaacgg gtaagcagat
     1681 actcgacccc ggcatttgca tcggctgcca gatttttcaa attcgtagaa tcagcgctct
     1741 catgataaat accctcagaa tcgcccacac cgcgcttgtc ataattcaaa gtggcaattc
     1801 cgcggctgag gaaataatgc gccgtcaatt tcgcgccaaa attatctcgt gtccccgctt
     1861 cagagccgtg cggcatcaca aaggctgcat gcggccccgc tgacgatggc atggtgagtc
     1921 gccccaccag cttcgttcca tccgcactaa agtagctgac gccgtcatcc atcacaaatg
     1981 ggactcgcgc cgcaaactct gaaggcacag gtgaagagag aatatcattc ggggtctgcc
     2041 accacatcaa accttcaata ttcccctgcc cgtctttcat gaattcgatt ctgccgtcga
     2101 agggggcgcc caccacgcga agcaccccaa ccaaaaaagt ggaatcatcc actgcataca
     2161 aactgcgcag gttgccgttt tcgaaatcgg tcatcatcag cgatttgccg aagtattcaa
     2221 gcccgctgga tgagaattca gggctgacaa tgatgctcag ggtacgccct gaatcaaaac
     2281 gatagacgcc ttcatagctg gcaagaattt catctgtcac agcagccagt tggaggaaat
     2341 gggtctggct tgtatgccca tcccaggtga tctcgccgga gatgagtcca ttttccgccc
     2401 tgccgctgaa ttcgatcctc tcgaacgggt cgcgggtgcg gccttctgcc aaaaagcgga
     2461 gttgcccgcc ttcctgtttc aattccaatt tccacttgcg ggtcattggc tcaatattca
     2521 gctcgggtat gtcttcatca aaattcacga tgaccgatgc aatggagcca tcgggcttgg
     2581 cggcggtgcc gatccactcc cctgtcagtg gcgggggtgt tgtagttggg cttggaatgg
     2641 gtgtgggaat cgctgtcggg cttgtacaag ccgtcaaaag gcagagcagg atcaaggtca
     2701 gggatatgat gcgcatggtc acaatatatc ggcgcatcca cgaaaagtcg ccctccaatt
     2761 ggagggaaaa tcaattaaag aagatccatc tcacgtgccc gtgctgccgc ctgcgttcga
     2821 ttatgcaccc caagttttcg atggatgtgt ttgacatacc agcgcacagt gctgaccgcc
     2881 agcgcgcgct tctgtgcgat ctcaggattg gaaaatccct gtgccatcag ccgcaagatc
     2941 ccaagttcct gttcgttgag cggttcgagc agcacaggca ttggctgcgg tttttgggta
     3001 tgaaaattgg tcaaaatggt ttgcgcaaat gcgcgtgcag aatgtgaatg cggcacacgc
     3061 ggcaaaagcg actcgatcaa ctcacccgca tcgagaaaag ggcgaatgaa tttttcaggc
     3121 tcagccagtg tcaatgcctg aaccagggcg gccagtgccg catcactctc tccctgtgcc
     3181 tgatacaaac aaacttgcaa cagacgcact ttgagaatca cacccacccg ttgttgtgcc
     3241 tcagcctcgc cgagacacca ttcgagcagc gcttccactt tttgatattc cgtcggcttt
     3301 gccagatcaa ttcgataccg acaggctgca taaaacgatt cttcattaaa cagcagcttc
     3361 gactgaacag gatcaaagga atcccaaccg ctggattttg cccaacgttc aaccgcctcg
     3421 gtcttccccg ctgaaaactg gtacagcgcc tgcaaacctt ttgcccgctc aaacagatct
     3481 gccacctgat gtttttgcgc aaaacggcgc gccttctcaa gagcatccaa cgcctgtgca
     3541 aattctcctt cagcctgatg cagcatggca agattcagat aagccctgac actgctggtt
     3601 gggtattcct tgaaattcag gttgtgattc ccctgcaaaa tatgttgtcg cgcttcgttc
     3661 aatttattcc aaagaaagtt cacctcgcca agatacccat gcgggatgaa ggcaagatgc
     3721 tgcatggatt cggcatcggc aagatgcagg gcgtcttcag caacttgatg cgctttatac
     3781 aattgtccgc gcgccatggc cagcaccgct tcatttatca gcatggaaaa ctcgatgaag
     3841 ggatgccctg tttggtaggc caactgtcgc gtagcgcttg ttacagaatc agcttgtgca
     3901 aaatcaccca cctcgatctg cgaagtggat aagccagccg atatgatcat ctgcaagtgg
     3961 gtgttgcctt tgggcacgat cctcaaagcc tgttcagcaa gttgcaccgc agtttgaaaa
     4021 tcatggcgat gccgtgcggc agtggcacga atcgccagaa tttgtgcatg ggagttgcga
     4081 ataccggggc caaattgctc agggtcggct gcaaccgttt tcaggtcagg tgaaagaacc
     4141 gcttcggcac ggtctgacca tcgttcggcc agttgcggcg aatcaccaag cgcgtacacc
     4201 catccatacc aaatacacaa ccctgcattc ttgctgacaa tttcatcagg tagttgtttc
     4261 attttttcaa ggatggtggc aacttctccg cggcgcatcc actcttgcga ttgcgggccg
     4321 atcaatgcag ccgcaaattc ataatcttga gcggcaaggg catgttcgat ggcctcggtc
     4381 acatatccct gcccagccag ccagcgggcg gcgcgctgat gcaggcctcg aattgcctcc
     4441 gctgggtatc tgatcaatgc ctgtttttga aggaagtcag caaacagatg atgaaaacga
     4501 aaccattcac gtgaatcgtc cagtgaaacg aggaagagat tttctgaatc cagttcatcc
     4561 aaaagggctt gggcgttgtt gcccccagtc agtgtgttgc acaaagcggc gctgaaacgg
     4621 gttacgagcg cggtctgcat gagaaaatcc tgtttttcgg gcggcaattg cccgagcacc
     4681 tcggccatca aataatcgga aagatcacga ttgccatctc cccatgacca ggctattccc
     4741 tgctgttttc ggctgcgcag ggatagggcg gccagctgca aaccagccgc ccagccttcc
     4801 gtctgttgtt gaaggtttgt caactcagca gcagggaggt caactttcat cacacggcgt
     4861 aaaaattcac ccgtttcttg tgccgtgaac tgcaaatcgc tggagcgaat ttcaacaagt
     4921 tgatgacgta cccgccattt tgccagcggc aggggtggat cattacgggt ggtaatgaac
     4981 aaccgtagat acggctgcaa gtattcagcg atttcagtca ccaaacggtg aatctcggcg
     5041 gatttgataa agtgataatc ttccaacacc agtgcaacgg ggtgttcaag cacagcacaa
     5101 tcgcgtaaca ggctgttgat aaccgcttcg ctttgcgggg tttgtccatc tccgcttgta
     5161 aaggctttgg cagcagacct gcctaaaact gggtccaacg attggagcgc tgcgatcaca
     5221 tacgaaacaa aacgcggaaa gagatcatcc tcatctccca atgagaacca ggccgcttta
     5281 tgctttttgt tttgcagcca gttgaccaac aaggtggtct tacccgagcc agcaggcgca
     5341 gagatcaggg tcaatgctgt gggggcggcc cagccttcat caaggatact gaaaatacgc
     5401 ggacggacaa ccacatccat gcgcggacgg gggatgttga tcttggtggg aatcatcgcg
     5461 gggacggtca tagtggatga agggtattat agttaatttg tgccattata aaaacatccc
     5521 gccgacagat cacgtcggcg ggatgggcgg tgacagatat ccgttctttt attggcggtt
     5581 tgaaaccatt tgaacaagag tggggatggt cagcgcggca aagaaagcag ccagcccaaa
     5641 tgaaagcgca ggcagcagcc agcgggcatt gacgatgaaa tcctgataaa gaaggatcca
     5701 ctgaatttgc agttttacca ctgccacagg cgggagactt tgcaaggcaa acaatacggc
     5761 gccaatggat aaggcaatga acaaactcaa tacaaaatca ttccacgtaa caagcgcggg
     5821 gcgttgttga gatgggacag cctgaatgcg tgacatcaca ttggctgtga tgctgcgcgg
     5881 catgggcgca agcggctggg acttaagcgc atcttcgatg agcgcatctt gttctaggcg
     5941 gggatccatg attcctccag ttgggcaagc gatttgcgga gttgttcctt ggcgcggaaa
     6001 atgcctgttt ttacggtacc aagcggcagg ttaagagtcg aggcgatctc ttcgtaggaa
     6061 agttcatttt ggtatcgaag agtaatcagc aattggtaat ttgagtccaa cgattgaatg
     6121 gcatcctgca aatgctttcg agtttcgctg atttcaaaga tttgagcggg ggtatccaga
     6181 tacggttcgg ggatgtcttc catcatgtca tcgccgaggt cgttgaggga gcggcgcagg
     6241 ttcggcaggc ggttgtagca caggttggtg acgatgcgat acaaccacgt gcggaattgg
     6301 ctctgcccct taaagttagg cagcgccgtc caggcgcgca cgaaagtctc ctgtgccaga
     6361 tcgagcgctt cgttctcatc tttgacgacg cgcaaggcaa ggttgtagac gtattgttgg
     6421 tgctcattca ccagcgcttc gaaggcttga acgtcgcctt tttgggcttt ttgaacgagg
     6481 atctgttcag agattaccat tttctactgg gttagacagg gaaaggggcg gaaaagtttc
     6541 agaagagcaa ggatgaatta tgaaggattt ttggcggctt cgtgatctgg cgtctttatg
     6601 tttactccac ggacccagct acgttccgtg gataaacgaa tttgaaactt tttcccgaat
     6661 cccctgtcaa acgtagtgac aaacataatt caaggagaaa caaaatgaaa agaccaattg
     6721 ttatcaccct attgattgta gcactcgtgc tggtatgcgc gggaattggc tcagtgatct
     6781 attttgccaa cggcttctca gtcaacaacc cgtttgataa gctgaatatt tcatccgtgc
     6841 tggaggaaag taaaaccctc aaggtggaca ccaccaaacc gattaagctg aaagtattgg
     6901 acgatgctgg caccgtcacc atcacaggtg ccgatgtaga aacagtgcag gtaaggatag
     6961 tcaagaccgc ttacgactca tcacaagccc gcgcggacgc ggaagtaaaa aacatcaaat
     7021 acaccatcga acaaaatggc gacaccatca cgctcaaata tgaactaccc aaatctgtga
     7081 actttagcaa ccacatcaac accgtggatt tcattgtcac cgttccaact gaagttgtcg
     7141 tgagtgtaaa caacagttca ggcgaagtca gtgttgctga caccaagggc aatgtcgata
     7201 tcaaaaacag ctttggcgac gtgaccattg agaatataga gggtgcctta acggttcaca
     7261 cgaatagcgg caaagtgact gccacatcca ttgtggcagg tacggaaaac attgatctga
     7321 gttcagactt tggcgaaatc atcttgaaaa aagctggcgg cacggatatt tccctcgact
     7381 caaacagcgg caacatcaca ttgagtgaag tccgtgtcac aggtgatctc acagcgaaca
     7441 cagatttcgg caacacagag tttgaaaacg gctcagctga tgcgctgcat gtcgaaacca
     7501 atagcggcaa agtatcgctc accaagttga aaattagcaa actccttttc gtaaagaacg
     7561 actttggcga tatcgaactg gatcaagcca tggcagattc ctatgatatg cacaccaata
     7621 gtggcgatat cacagtggac ggcgccaaag gcaaactgaa agccgataca gattttggca
     7681 acgtcaccat cagcaatgcc cagtccgtta cattgaccgt tgagacgaac agcggcacca
     7741 tagaattcag cggctcgttg ggcgtggggc cgcacatggt caagtctgat tttggcacca
     7801 ttgatatcag cctgcctgcc gatgtgaaat tgaccgtgga tctcaaaacc gattttggca
     7861 agatcaaatc agagttgccc gtcacggtca tcctgactga cggcgccagg tcagacggcg
     7921 accagatcgc tggagatatc aacggcggcg gcgaacaact cactgtgcaa gccaacagcg
     7981 gcaacatcac catcagggtc agtagtcaat aaaaccataa aaaattcggt gacagcgggc
     8041 ggctgtcacc gaatttttta tatgctatca ttgtcaggaa aggactcgac atgaacgata
     8101 tcattccctg tcttggcgtg gttggctcac tcaccatcat ctttggcttt ttcgcctttc
     8161 tccgttatat aaattacaaa gaaaccatcg cccttgctga aaagggatta gtcaaatcag
     8221 aatcaaaacc aaacaaaata cttttgcgtt gggggattgt tatcaccgcg cttggcttcg
     8281 cactctcact cggactctac accctcggct ttgcctctgc cgataactac ccgcttcacc
     8341 tcggaccctg gatgctgggc ggcttcgttc cgctttttct tggcctcggc ctggtcctgc
     8401 ttcactacct gaccgagaag gaataatcca ctagcaaatc ctcggcagca tttcacctgg
     8461 cagcatatca attaatcggg ttcccccaat ggcggtccgc aatctcactt gcggactttt
     8521 tgtttcgatc accttcccaa tgatgcaagc ctcttcgcca tattttgtct tccgcatggc
     8581 agagaggatg gattccgcat ctgctgcttt gacaatggcc agcagtttgc cttcgttggc
     8641 aatattcaac gggtcgaagc cgagcatttc acaagccgcc cgcacctgcg gtttgacagg
     8701 taatgccgtt tccagcaatt cgatccccac tttcgattgt gaagcgattt cattcaaggt
     8761 agtggccact ccaccgcgag tggggtcacg cagcacatgg atttcacctg ctcccatcat
     8821 cgccgcgacc aaatggttca acggcgcaac atccgattgc aggtcggttt caaattgaag
     8881 attctcgcgg gccgataaaa ccgccactcc atgatcgccc atggtcccag acatgatgag
     8941 gacatccccg atctgtgcat tcgcgccaga aatatttaca cccgtctcca ccacgcccac
     9001 ccctgaagtg ttgatgaaga gtttatctgc gccgcctttt tgcaccacct ttgtatcgcc
     9061 agccgcaata tacacccccg cctccgccgc cgattcgcgc atggacctga ccacttgttg
     9121 cagaatctcg aaagacaggc cttcttcaac tacaaatccg caggtgaggg caatgggtct
     9181 ggcacccacc atcgccagat cgttgaccgt accgcaaacc gccagcctgc cgatatcacc
     9241 ccctgggaag aacaacggag aaacgacatg cgaatccgtt gtcagcgcca gccgctgccc
     9301 gttgatgttc aacacagccg catcgtccag tgcgcgaggc gcggcctttc caagatcagg
     9361 cagaaagagt cggttgatca agtcgtggct tagttttcca ccggagccat gtcccagcac
     9421 cacggtatcc cgaggttgaa caggcacagg gcaggtatag ccttcaaaat caatttcgtt
     9481 ttccatcaat aacctcttca cctattcccg atgataccga taataggccg cacaagcccc
     9541 ttctgctgaa accatcggcg cgcccagcgg agaggatggt gtgcattgtt ttccataagc
     9601 aggacattgc ggcggcttct tcataccttg caggatctcc cctgcaatac ataaaggcga
     9661 ttcctgtgtg atgatctcac ccacagagaa acgcgcttcg gcatcgaact cggcaaagtc
     9721 ggggcgcagc ccccagccgc tttgcggaat taaaccgatg ccgcgccact ttcggtccgc
     9781 aggttgaaaa acggtctgca ccacctgttg agcagcttga tttccctcaa aggttacggc
     9841 acgctcgtag gcattctcca cttctgcctt gcctgactcc aattgcttca ctgccatgag
     9901 cacaccgcgc aacaggtcca ctggctcgaa cccggtgacc acgatcggca ccttgtattt
     9961 ttctgcgatc gcaggatatt cgtgataccc catcaccgcg cagacgtgcc cagccgcgag
    10021 aaaagcctgc acacggttgt gctttgaacc caaaatcgcc tccattgcag gcggcacaca
    10081 gacgtgtgaa acgagcaccg agaaattctt aattcccttt tgtttggcct gcaacacagc
    10141 cattgcattg ggcggcgccg tggtctcaaa accgatcgca aaaaagacga tctctttttc
    10201 ggggttttcc tgcgcaagtt tcaacgcctc cagcggcgag tacaccacct ttacattccc
    10261 accttttgca cgcacattga acagatccac actggagccc ggtacacgca acatatcgcc
    10321 atacgaagtg aagatcacct caggacgggc agcaatcgcc aacgctttgt cgatctgctc
    10381 cagcggggtc acacagactg ggcagcccgg gccatgcacc aattcaatgc cgccaggcag
    10441 catctcatcc aacccatgat gcaggaaggc gtgcgtttgt ccgccgcaga tctccatcaa
    10501 cacccatggc tgggtgacag tttcgccgat ctctgccaat aatccgcgca cttttgcagg
    10561 gtcgcggtat tcatcaatat atttcataca ctcccaagtt cctcccccaa tgcatcgatc
    10621 tcatggatca tctgcagcct ggcttgcgcc tcctcctcat caagcacact gatcgcaaag
    10681 ccgacatgga tcaacacata ctgccccacc tgaacctcag gaacatacgc aaggcaggct
    10741 tcctttacga taccgccaaa gtccactttg cccattttga gcgaaccctc atcatagatt
    10801 ttttgcagct tccccggaat gccaagacac ataacgaact ccttattgaa aaatcctgct
    10861 gttttttgaa acacatcgtt tctggtctgt ctatctttca gacgagtaat ttgcgatcac
    10921 ggcctgtccc aatgccagac ctccatcgtt tgcaggtgtt tgccgatgcg agaacacaac
    10981 aaaacgttca tccgccaatt tctcacgcgt caagttcagc agggtttggt tttgccaaac
    11041 accgcctgaa agcgccactt cattgattcc tgtttcattg cgcgtgttcc tgcaaacttc
    11101 aaccgccaag gcacacagag tctgatgaaa ccgcgcaccg atcaacccta caggttgttt
    11161 tgacctgatg tctgcgatga cgtcttgaag cagttcgccc aacaacacga tcccgttcct
    11221 gatctgatat ggataggcct ctgccccgtt gatgaatcgc ttcgaaagaa cttccaactc
    11281 gattgcggct tgtgcttcgt aactgacatc gcttcgcacc ccgcccaggc tggctactgc
    11341 gtcgaacagc cgacccatgc tcgaagtatg cggcgtgttc aaatccttgc gcacctgctg
    11401 acggacgatc tctacagcgg tcgcatccag tccctgcatg aagggcaggt cgttgatctc
    11461 cagcccaagg gcttctgcat atccaacagc aatgcgccac gggtggcggg tggcagagtc
    11521 actgccgggg agtgggagat attgaaggtg tgcaacccgc tcaaagtccg cgtaggaagc
    11581 gagcaatact tccccgcccc agatcgcccc gtcggttcca tagcccgttc catcgaaaga
    11641 caatccgatc actctgcggt ctgtgagcct gttatcggcc atgcaagcgg caatgtgcgc
    11701 atgatgatgt tggacaggca cccgcctgcc ttccattttt tgcgcccact gagtcgtgaa
    11761 ataatttgga tgcatatcat gagcaatgac ctgcggctgc acgcggaaca gatgcgaaag
    11821 ccgctcaaca ttttgctcaa aagactcgta cacttcgaca ttttccatat cgccgatgtg
    11881 ctggctcaga aacgcgtagc ggtcacgtgt gaggcagaag gtattcttca actcgccgcc
    11941 cactgccagt gtgggcctgc tctcgaatgg cagcaaaacg gggtatgggg catatccccg
    12001 cgcacgccgc aggaaggtta tgccgcggcc atcggcacgc accaccgaat catcgcagcg
    12061 cacgtggata tcgcgattgt gcaacaggaa ggcatcggcc agcggggaga gtttttcaaa
    12121 cgcatcttca tttgtgatgg agatgggttc ttcgctaagg ttaccgctgg tcatcaccag
    12181 cacaggcggc acagactggg cgtttagttt ttcatctgtc tgattgagaa tgagatgatg
    12241 aagcggtgta tatggcagca ttgcgccgag ggtatccatg tgcggagcaa cattggcagc
    12301 caggctggta tgtgccttga gcttcaataa aacgatcggt ttttcatgcc cggtcagtaa
    12361 tttcgcttcc tcttcattga cttcgcaata ttgcatcaca gtatcgaggt tggcaaacat
    12421 cagcgcaaat ggcttgcctg ccctgccctt gcgttgacgc aattcattga cagcgcgttc
    12481 gttcatggca tcgcaggcaa gatgaaatcc gcccaaccct ttaatggcaa caaccctgcc
    12541 ctcccgcaga aattgacgcg ttttcaagac cgccgaaagg cgcaattcaa tattcgaaac
    12601 atgccccgta gacaggcttc gcaattggac aaacggtccg caagtggggc aggccacagg
    12661 ctgggcatgg aatcttcggt cggttgggtt ttcgtattca gcacggcaat cttcgcacag
    12721 ggggaaatct gccatcgtgg ttgcaggccg gtcatatggc aggtctttga taatggtgaa
    12781 acgcggacca cagttggtgc agttgatgaa cgggtacagg tagcgcctgt tttgcgggtc
    12841 gaagagttcg cgctcacaat cggcacaaag ggcaacatca ggtgaaacgg gctggaatgc
    12901 accttcaata tgatttgaag gttgaatggt aaaaaccgaa tggttttcaa cctcagcctc
    12961 tttaactgtg accgaatcca gcgttgccaa tggaggcagt tcactcgaaa gtttttcaat
    13021 gaataaagcc acctgtacct ccgccccttc cacatgaata tccacacctg ccgatgtatt
    13081 gagcacccag ccacgcaaac caaggcggac agccagcccg tacacaaacg gacggaagcc
    13141 cactccttga acgatgccgg taacgtgaac gcgaatgctg cgcattggag aaattagtgg
    13201 atcatttgct ttttatattt tgtacaagcc agtcggtcca tgcttccatg ccttccccag
    13261 ttttggcaga caccgcaaac gtcaccagcc ccgggttcag cacttccaca cccttgcgga
    13321 aatattccat attgaaaggt acatagggca gcagatctat tttattgatg atgagtgcct
    13381 gcacatcgcg gtacatgccc gggtacttat acggtttgtc gtcgccttcg gggatggaag
    13441 cgatcaacac gttgagatgg gtgccgatct gaaaattagc cgggcagacg agattgccca
    13501 cattttcgac gaggagcaga tcgatctcac ttaacgggag agttggtaag gcatccgata
    13561 acatggccgc atcgagatga cattctccgc cggtgttgat ctgcacagcg ggcaaacctg
    13621 ccgccgcagc tttttccgcg tcaatggatg aggccaggtc accttccact acgccgagtt
    13681 ttaaccgtcc gcgcagggtt tcgagggttt ttaagatagt gctggttttc cccgctccgg
    13741 gggaggccat gatattgatg gcaaataccc ccgcctggtc aaggaaattg cgattacgct
    13801 ctgccatgcg gtcattggca gagaggatct tttctacaac tgggatgcgt gtggtcattt
    13861 ttctccgtgg tatgcgtcta ttcaacgtca atgctatcca cccgaaattc ctcaccctgg
    13921 gtgatgcgta cctgcatact tgcacaggat gggcattcga aggtttcatc atcggggtgg
    13981 aaggtatgcc cgcaggcgct gcaagccatt tctgcgggga tgcgttcaaa gtggagcctt
    14041 gcaccctgcg ccatggtccc ttcggcgaca atattccagt agaagctgac tgaatcatcc
    14101 acaatggatg cgaattgccc gatcacaagg ttgatatctg tgactcggct ggctccagcc
    14161 tcttttgcat ggcggaggga aatatcgagg atgctttcag taacggcaag ttcgtgcatg
    14221 gcaaccacct taattattgc cagaaaaggg attttcataa gtgaataaag tcattccatt
    14281 aatggacaat tttcctctga tttgttgatc ctccagaggg gagaaaatgc caattgagag
    14341 tggacaggta acgggtccgt attgaacgcg ggcgagggtg atgaaatccc aaaagggatg
    14401 catccacaat ttgtgtagtc attcccatcc gcttgttgtc tctttcggtt gaaagttgat
    14461 agtgtttgat agccccgctg gataaaatta gacttgaaga aaggaactaa accatggaaa
    14521 tcccacgaca ctggcgtctt aaacaacaac gatatggatt ggtaggcgag gtctgcccac
    14581 actgcgatta caagattttt cctccgcgtg atgtgtgccc caactgcgga gacgaggcca
    14641 aggaattatt cacttttagc ggcaagggcc aggtttattc gtacaccact gtttatgaag
    14701 ctccagctgg ctatgatgca aatgcccctt acaccgtggc aatcgtaaaa ctagatgaag
    14761 gtcccatggt gacggctcaa ttgaccgacg tggacaattc cgaagtccag atcggcatgc
    14821 ccgtggaaat ggtcacccgc aagatgcg
//
LOCUS       DAOQLC010000105        14650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1493_length_14650_cov_21.424872, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000105 DAOQLC010000000
VERSION     DAOQLC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_14650_cov_21.424872"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PL000_16045"
     CDS             complement(<1..546)
                     /locus_tag="PL000_16045"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC90445.1"
                     /translation="MAKVFISYSRKDIDFAKRLTGELQKSELDFWVDWEGIPPTVDWW
                     REVEKGIEEADVFLFLISPDSVASKVCGQEIEHAAKNGKRLIPLVVRDIRGEEAPNHL
                     RHLNWIFFRESDDFEASLKKLLEGIHTDYEWVQSHRRLQVKALEWERNNKETSFLLRG
                     RDLQDAEFRLATNTSKEPYPTD"
     gene            complement(626..2452)
                     /locus_tag="PL000_16050"
     CDS             complement(626..2452)
                     /locus_tag="PL000_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNC90446.1"
                     /translation="MYGWHLKILRVNLTTHKVTTEDVDPKIARDYLGGRGWAIHYLYK
                     EMDPMADPLSPENKLIFATGPLTATPAPTGNRYMVVTKSPLTGALAHSNSGGEFPTWM
                     KRTGFDLYIFEGRSPEPVYLFVNEDQVEVRSAAHVWGKDTHETTDILKAETSEEARVA
                     CIGPAGENLALMAAIMNDKHRAAARSGVGAVMGSKNLKAVVAMGSKNPPLHDPEGMRT
                     LSVETSKNVGADVKKGSNMRIYGTSYVPQVTNTLGILPTHNFLQGTFEHVHNVDGDAL
                     KNQYLIRHTPCYRCPLSCGRLTEVPDGPYKGKGEGPEYETISSLGTGCGVSNLAALVK
                     ANYLCNEYGMDTISTGMTIAAAMEMYEKGYIPEEVIGQPLKFGDHDAMIAMIKLMAYN
                     EGFGKELAQGSYRLAEKYGHPEIAVTTRKQEFPGYDPRGSQGMGLLYATSNKGASHME
                     GDVAYEEVFGVPVKENPLTTEGKPELVKHFEDSFALMDSSGVCVFVGIRYMFNKDRMI
                     LPQTLANMMNLSTGAGYTAEEALRAADRVYTLERMFLLKAGSTEDTLPHRMLHEPLPD
                     GPAKGKVVELDKMLPEFYKLRGWDEKGWPTKEKLADLGLPLN"
     gene            complement(2549..2788)
                     /locus_tag="PL000_16055"
     CDS             complement(2549..2788)
                     /locus_tag="PL000_16055"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC90447.1"
                     /translation="MMIRVKLIANYRAALPPEAEHGVVELNVPDGTTVYDAISRFDIP
                     LNDESVIVLNGLTVDMNTPLQEGDMVTAFSAIAGG"
     gene            2827..3267
                     /locus_tag="PL000_16060"
     CDS             2827..3267
                     /locus_tag="PL000_16060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC90448.1"
                     /translation="MKRQIPYHGWCFVCGNENPRGIGITMFVDDNGILTSEFTLNETH
                     QGPPGYSHGGASAAILDEVMGLVVWAAGHKVVAANININYRKPLPLHQPLLAEARISE
                     VGERKITSVGEIRLADSTVAVSGSGVYVVAPHLFEAKRLKGVVS"
     gene            3264..3962
                     /locus_tag="PL000_16065"
     CDS             3264..3962
                     /locus_tag="PL000_16065"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HNC90449.1"
                     /translation="MKTIGLIGGMSWESSIEYYRIINETAKEKLGGLHSAKSLMVTVD
                     FAEIEKLQHEDRWDEAAQILVQCAQDLEHGGADFIVLCTNTMHKLADQITANVSIPFL
                     HIADATAEKIRAAGLKRIGLLGTRFTMEHDFYKGRLVQNFGLEVLIPEQTDRDIVHRV
                     IYDELVQGKIVETSREEYKRIIEWLIEQGAEGIILGCTEIELLVKQNDSRVPLFPTTL
                     IHAVAAVEKALQED"
     gene            3969..4424
                     /locus_tag="PL000_16070"
     CDS             3969..4424
                     /locus_tag="PL000_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90450.1"
                     /translation="MRIFHAIFFSLLLSACLTTPGPDTTPTADSPFVTVQEENPYSPK
                     PEDLGKQQASVIVTSVDLSERSDLSPVRVEVSIFGSMPSVCNELRVTVDPPTAAYEVH
                     IEAYSVADPNQNCENVFQQFEGHILLGVYTPGRYTIWVNDTFVGDMISN"
     gene            4500..5183
                     /locus_tag="PL000_16075"
     CDS             4500..5183
                     /locus_tag="PL000_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90451.1"
                     /translation="MFNFFKKKEKKPETSLADALRDVIFGVAPATEWPKGDTGEHPWS
                     VFIEARRHIMERQNFEEAAKLYKQITETPGLESRHYLQAWFFLRKMNIQPPADMARKA
                     YGIVVDVRLQSGPELVACYADHTARYLHSSGGGIVWEAPDTSLNDVIDALIRAGDEVA
                     SKVPPLENNIRPNAPQQVDAVQIGVLTPSGIHHGIGTFEGWSKDPMGSTIVNAASYLM
                     QNLVNKKKS"
     gene            complement(5272..6381)
                     /locus_tag="PL000_16080"
     CDS             complement(5272..6381)
                     /locus_tag="PL000_16080"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC90452.1"
                     /translation="MIPGLDGLRAVAFLLVLGAHTDNLSFGWVGLQLFFVLSGFLITG
                     ILIRMKNSLGKREYFTKFYGRRFLRIVPLYYFYIFLMFGLKFAAPVLEFKPLLNEFGD
                     KFWLQVWLSIFYVYDFFHTTSAYEHSRFFTHLWSLSVEEQFYILWPLIVFLTPNEKMK
                     RLFFGAIALGPIMRGVVYWVYSYHRLPFMTTIPDIAVYAMPFSHIDAFAFGAFASQFE
                     IPRPRLQLFLASILVPAIGLSTQYLVEGRALWSTLGQGFLFSVGYMQIWGYSLLNYLF
                     MVLIYNVAKTGLFNHTLDLPPLRYLGKISYGLYVYHTAAIWLVLKLGSIDSGNMVLYG
                     ISFVVTAVVASLSYQVFEKPINDLKDKYFPLSVSA"
     gene            complement(6425..7420)
                     /locus_tag="PL000_16085"
     CDS             complement(6425..7420)
                     /locus_tag="PL000_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90453.1"
                     /translation="MRKLSLIHLSILFLVAACNLPVATPVPTPHLFQTMQASTPVAVD
                     TAAPTAPVVEEPSEPTGKIAFTCQVFKELEREQICMVNADGTNYQRLTTEDDTRHFYP
                     SISPDGLSVLYSALDEQSKHYEIYEFDLAAGEPKALTFALGDLNAPEISPDGNSIAFT
                     RFFDDPEHPTTWLMDRDGGNPRQVSDVRAWDPTWSPDGTQILFASYADGLSQLFIINL
                     DGSGLRQVSHLPALRGRSDWSAAGLIVTYSGKPWERELFSMNPDGSDLQQISPAGGNS
                     QGPSFSPDGEWVAFTAYFDRFEVHGCEIYIMRVDGTDLRRLTDNDYCDYQPRWGP"
     gene            7569..9422
                     /gene="htpG"
                     /locus_tag="PL000_16090"
     CDS             7569..9422
                     /gene="htpG"
                     /locus_tag="PL000_16090"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNC90454.1"
                     /translation="MANDAPQQYTFKAETKQLLNILIHSLYKDREVFLRELLSNASDA
                     LNRLRFEMVTNQNILDSNAELQIRIKTDKDARMLIIQDTGIGMTKDEVIENLGTIAQS
                     GARKFLDATKDKKVDFTQVIGQFGVGFYSVFMVAEWVRVTSRSFRPDANAVSWYATGE
                     DNYEVSEADMSERGTRIEIKLKEDAAEFAEEFRLKNIIHKHSDYIGFPIYMGDGTEAV
                     NKQTSLWRTSRQEIKEDQYKEFYRQTTLDFEDPLLHTHMVTDAPVQLYALLFIPAKAE
                     RGIFALRKEDGLKLYSRNILIDDYNKDLLPEYLRFVQGVVDSEDLPLNVSRETVQSSG
                     LMPKLKKVLTNQVMRELELLAKNNAEKYHTFWQEFGPYLKQGVAANLAEADAIKPLLR
                     FKTNLNPETWTSLEEYASRMKPEQKEIYYIIGEDPKSVLRSPHLDYFHSQGTEVLLLT
                     DPMDSFMLMGLRKYKDLDLKNVAQADVDTAKQSTEQPDTEKISGDEFNTLIERFKQVL
                     GDRVTDVRASTRLVQSVARLVDTDANANPDMQRVYKYLGKEYEIPKKVLELNPSHTIL
                     KNLLHQESDSELQNIIIEQIYDSALLVEGLHPDPSSIAERVQQIMEAALKK"
     gene            9648..10274
                     /locus_tag="PL000_16095"
     CDS             9648..10274
                     /locus_tag="PL000_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90455.1"
                     /translation="MKKQTTLFSVLLVILALFFSNTQPVGAAAPAVISAALPNPGAII
                     PVAAGKTATYSQGPHTSGFPSSWGIVNPGKVTSSIDLGYSGTVVAPISGYLAIMKDCG
                     DHQVAVVRASNGWAIGLTHIFVNTTVRNKSVSQGQVIGKTVPPPATSKGCGYGKGSHI
                     HYTLMKWVQSGTIKYTEQDIVNTYFSKWIVKNTYLDGPTRIPIGGVIK"
     gene            10383..11012
                     /locus_tag="PL000_16100"
     CDS             10383..11012
                     /locus_tag="PL000_16100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90456.1"
                     /translation="MDIQTAYNEWSGIYDTNTNLTRDLDSKLTRDLLAHQKFDSILEL
                     GCGTGKNTIFFSQIGKKVHALDFSPGMIEKARTKVSASHVRFEMADLTKHWPCQNEAY
                     DLISCNLVLEHIAELPHIFSEAARVLHRGGKFLINELHPFKQYQGTKARFERGTETVE
                     LEVFVHHISEFITTALKHDFKLLHFNEYWHPQDENKPPRIATFIFEKQN"
     gene            complement(11023..12180)
                     /locus_tag="PL000_16105"
     CDS             complement(11023..12180)
                     /locus_tag="PL000_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90457.1"
                     /translation="MSFDEVAVLLPDLTLTLRANLSNWYGIGTSIISSPDVRMREWSV
                     FVRYQIQLADLQRRAILVKIRRHPDQSISESISSPGLKEEGRNDYEALKAIENFVSTA
                     ENIPGENNNTNPYGYFSSEEKDHFCAVRPLAFFDPFSALVMEELEARPLRNMILNFKT
                     AYHAGWQEQLSNWFYRSGRWLRVYHEQLGKLHEGPFFSDDIHAALEKSINVIEPFHDF
                     DFTLVRNVLRQTRAAYKDHNLSFGLLHWNFDCANFLVAEDNRIGILDPHFIDGPIYMD
                     IAKIMTDLQTYRLQTLSYGLFIREKVMQKFHRSILQGYFGSEQPDEVAINLYCIYAVL
                     DKWQIDEEAEHEHGNEHGWLHQFIQSHATWRRKYFMKLLSAYVDRLQKLLP"
     gene            complement(12473..12994)
                     /locus_tag="PL000_16110"
     CDS             complement(12473..12994)
                     /locus_tag="PL000_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90458.1"
                     /translation="MLTSFWFAGGGTFWQPIDYFPHPVETILNLQPYGNEFWVTTTDG
                     ATYHILYPCQEAAGCWQEDEAVSDLVPSDAYVVSEGRCESDYFLYPLFQQEIKSCVTS
                     TIFAETPQGSSISTIASPWRVSLALTEDKKLWIWQKPWSFPDRVLAFKVFFTALGLLV
                     GTMVDTLLTRKYK"
     gene            complement(13163..>14650)
                     /locus_tag="PL000_16115"
     CDS             complement(13163..>14650)
                     /locus_tag="PL000_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC90459.1"
                     /translation="FPGEYPYTRGVQPTMYRSRFWTMRQYAGFSTAEESNKRYRYLLA
                     QGQTGLSVAFDLPTQIGYDADDPIAQGEVGKVGVSISSIRDMEQLFDQIPLEKVSTSM
                     TINAPAGVLLAMYIAVAKRQGADMRQLRGTIQNDILKEYVARGTYIFPPAPSMRLITD
                     IFQFCAAEVPFWNTISISGYHIREAGSTAVQEVAFTLANGMAYVEAALKAGLNVDDFA
                     GQLSFFFNAHNNLIEEVAKFRAARRMWARIMRERFKAQKPASWQLRFHTQTAGSTLTA
                     QQPENNVVRVTLQALSAVLGGTQSLHTNSMDEALWLPTEKSVRVALRTQQIIAYESGV
                     ADSIDPLAGSYLIEHLTDEIEKGALDYIAKIDEMGGALQSIERGYMQNEIQNAAYAAQ
                     QAVERKEQIVVGVNQFSVDEVLTLERLKVDPSIELGQREKLKKLRDARDSGRVTALLG
                     KLTEAAKGTENLMPLFIECVENDITLGEVCNALRGVWGEYVAEGF"
BASE COUNT         4034 a         3819 c         3388 g         3409 t
ORIGIN      
        1 atcggtggga tacggctctt tcgatgtatt cgtcgcaagc cgaaactcgg catcctgcaa
       61 atccctgccg cgcaggagaa aactggtttc cttgttgttc cgctcccact ccaacgcctt
      121 gacttgcaag cggcggtgac tttgcaccca ttcgtaatcc gtgtggatgc cttccagcag
      181 tttcttgagc gatgcctcga agtcgtcact ctcgcggaag aagatccagt tcaaatgtcg
      241 caagtgattc ggcgcttcct ctcccctgat atcgcgtacc accaacggaa tcaagcgttt
      301 gccatttttt gcggcatgct caatttcctg tccgcacacc tttgaggcga ctgaatcggg
      361 gctgattaga aagagaaaga catcggcttc ttcgatccct ttttccactt cccgccacca
      421 atccacggtc ggcggaatgc cttcccaatc cacccagaaa tcaagttcgc ttttctgcag
      481 ctcgcccgtg agtctctttg caaagtcaat gtccttgcgc gaatatgata tgaatacttt
      541 cgccatgatg tattccctcc gctgttgagt caagttttaa tgacaaaaaa gaccctaaag
      601 gtttgaaacc tttagggtct gtaaactaat tcaacggcaa tcccaaatct gctaattttt
      661 ccttcgtcgg ccagcctttt tcatcccaac cgcggagttt gtaaaactcg ggcagcattt
      721 tgtcgagttc caccaccttg cctttggcgg ggccatcggg cagcggttcg tgcaacatac
      781 ggtgcggaag ggtatcttct gtggaaccag ctttcagcag gaacatgcgt tcaagggtgt
      841 acacacggtc ggcggctctc agtgcttctt cggccgtgta cccagcgccc gtggagagat
      901 tcatcatgtt cgccagcgtt tgcggcagga tcatgcgatc tttattgaac atgtaacgaa
      961 taccgacaaa cacacacacg ccggaagaat ccatcaacgc gaaagaatct tcaaagtgct
     1021 tgacgagttc aggcttacct tcagttgtga gcggattttc cttcacaggc acgccaaaga
     1081 cttcttcgta tgccacgtca ccttccatgt gggaagcgcc cttgttggag gtggcgtaca
     1141 gcaagcccat cccttgcgag ccgcgcggat cgtacccggg gaattcctgc ttgcgagtgg
     1201 tgacggcgat ttcggggtgg ccatattttt ccgcaaggcg atagctgccc tgggcaagtt
     1261 ccttaccaaa gccttcgttg tacgccatca gcttgatcat tgcgatcatc gcatcgtggt
     1321 caccaaattt cagcggctga ccgatcacct cttctggaat gtatcccttc tcatacattt
     1381 ccattgcggc ggcaatggtc atgcccgtgg agatcgtgtc catgccgtat tcattgcaca
     1441 gataatttgc tttcaccaat gcggcaaggt tgctcacccc acagcctgtt cccagcgacg
     1501 aaatggtttc atattctggg ccttcccctt tgcctttgta gggaccatcg ggaacttccg
     1561 tgagacgccc gcacgaaagc gggcagcgat aacaaggtgt gtggcgaatc agatattgat
     1621 tcttgagcgc atctccatcc acattgtgaa catgctcaaa agtcccttgc aggaaattat
     1681 gcgtaggcaa aatgcccagg gtattcgtca cttgcggcac ataagaagtg ccataaatgc
     1741 gcatgttcga acccttcttc acatctgcgc ctacgttctt ggaggtttcc acacttaggg
     1801 ttcgcattcc ctcggggtca tgcagcggcg ggtttttact ccccatcgcc accacggctt
     1861 taagattttt actgcccatc acggccccca caccagaacg ggcagccgca cgatgtttat
     1921 cgttcatgat cgccgccatc aacgcaaggt tctcacccgc aggcccaata catgcgactc
     1981 tggcttcttc ggatgtttct gcctttagga tatcggtagt ctcatgcgta tccttgcccc
     2041 aaacatgcgc ggcagaccta acctcaacct ggtcctcatt cacaaacaga tacacaggct
     2101 caggcgacct gccttcaaaa atatacagat caaaacccgt gcgcttcatc caggtcggga
     2161 attcgccgcc cgaattggaa tgagccagcg cacccgtcaa aggagatttg gtaaccacca
     2221 tgtaacggtt gcctgtcggt gcgggggtgg cggtcagcgg acctgtggca aagatcagct
     2281 tgttctcagg tgaaagcgga tcagccatcg ggtccatctc tttataaaga taatggatcg
     2341 cccatccgcg cccgccgagg taatcacggg cgatctttgg gtccacatct tcggtggtga
     2401 ctttatgagt ggtgagattt actcgcaaaa ttttgaggtg ccagccgtac atagggttct
     2461 cctgtggaaa aagatgggta ggaagggtgt agttacaggg tgaagggttt ttctttgcgc
     2521 ttcttcgtat ccttcgcggt caacgattct atccgccagc gattgccgaa aatgcagtga
     2581 ccatatcgcc ttcttgaagc ggggtattca tgtccaccgt caggccgttc aacacgatga
     2641 cgctttcatc gttgagggga atatcgaagc gtgaaatggc atcatacacc gtcgtcccat
     2701 cagggacatt caactcaacg acgccgtgtt ccgcctcggg cggcagggct gcacgataat
     2761 tggcaatgag tttgacgcgg atcatcattt gtgaatttta gcatgaaagc ggataaaata
     2821 attggcatga aaagacaaat tccctaccat ggctggtgtt ttgtgtgcgg aaatgaaaac
     2881 ccacgcggca tcggcatcac catgtttgtg gatgacaacg gcatcctcac atctgaattt
     2941 acgttgaacg aaacacatca aggcccgcct ggatattctc acggcggggc atccgccgcc
     3001 attttggatg aggtgatggg gttggtggtt tgggcggcag gtcacaaggt ggtggcggcg
     3061 aatatcaaca tcaactaccg caaaccactt ccgctgcatc agccgcttct ggcagaagca
     3121 cgcatcagcg aggttggcga aagaaagatc accagcgtag gagaaattcg tttggctgat
     3181 tcaactgtcg ccgtgagtgg aagcggggtg tacgtcgtcg caccgcattt attcgaagca
     3241 aaacgtctaa aaggagtcgt gtcatgaaaa ccatcggcct gatcggcggc atgagctggg
     3301 agtcatccat tgagtattac cgcatcatca acgagaccgc aaaggaaaaa ctgggcggtc
     3361 tgcattcggc aaaaagtttg atggtgaccg ttgattttgc agagatcgaa aaactgcaac
     3421 acgaagaccg ctgggatgaa gctgcccaaa ttctggtgca atgtgcgcaa gaccttgaac
     3481 acggcggcgc cgattttatt gtgctctgca ccaacaccat gcacaaactg gccgaccaga
     3541 tcactgccaa tgtaagcatc ccttttttgc acattgcaga tgcaaccgct gaaaagatcc
     3601 gtgccgcagg cttgaaacgc atcgggttgc tcggcacacg cttcaccatg gaacatgatt
     3661 tttacaaggg ccgccttgtt caaaacttcg gtcttgaagt tttgattccc gaacaaacag
     3721 atcgagacat tgtccatcgc gtgatctacg atgaactcgt gcaaggcaag atcgtggaaa
     3781 catcacggga agaatataaa cgcatcatcg aatggctgat cgaacagggc gccgaaggca
     3841 tcatccttgg ctgcaccgag atcgaattgt tggtcaaaca aaacgacagc cgcgtgccgc
     3901 tcttccccac tacgctcatc cacgcggtgg cggctgtgga aaaggccttg caggaggatt
     3961 gacccctcat gagaattttc cacgcgatat tcttttccct gctcttgagc gcgtgtctga
     4021 ctacgcccgg gcctgatacc acgcccacag cagattcgcc ctttgtcacg gtgcaagagg
     4081 aaaaccctta ttcgcccaaa cccgaagacc tcggaaaaca acaagcaagt gtcatcgtca
     4141 cttctgtaga tctctccgaa cgttcagacc tctctcctgt acgggtggag gtcagcattt
     4201 ttggttccat gcccagtgtg tgcaacgaac tgcgcgtcac cgtagacccg ccaaccgcag
     4261 cttacgaggt tcatatcgag gcctacagcg ttgccgaccc aaatcaaaat tgcgagaacg
     4321 tcttccagca atttgaaggg catatcctgc tcggcgtgta caccccagga cgatacacca
     4381 tttgggtaaa tgataccttt gtgggagata tgatctccaa ttaattgacc atatttccca
     4441 ttcacgccac ctaaaatctg gatatactcc tgccagccca aaaacaggag atatcaatcg
     4501 tgttcaattt tttcaagaaa aaagaaaaga aaccagaaac aagccttgct gatgcattgc
     4561 gtgatgtgat cttcggtgtt gcccctgcaa ctgaatggcc caagggagat acaggtgaac
     4621 atccgtggtc ggttttcatt gaagcgcgcc gacacatcat ggaaagacaa aatttcgaag
     4681 aagccgcaaa actttacaag cagatcacag aaacgcccgg acttgagtct cgtcactatt
     4741 tacaggcatg gttcttcctc cgcaagatga acattcagcc gcctgccgat atggcacgga
     4801 aagcctacgg catcgttgta gatgtccgct tgcaaagtgg acctgaattg gtggcttgtt
     4861 atgccgacca taccgcccgc tacctgcata gctctggggg cggaatcgtt tgggaggcac
     4921 cagatacctc cctgaacgat gttattgatg cgctgatccg cgcaggagat gaggtggcat
     4981 cgaaagtccc gccgttggaa aacaatatca ggcccaacgc cccgcagcaa gtggatgcgg
     5041 tccagatcgg tgtactgaca cccagcggca tccaccacgg cattggaacc ttcgaaggct
     5101 ggtccaaaga ccccatgggc agcacgatcg taaacgcagc cagttatttg atgcaaaacc
     5161 ttgtcaacaa gaaaaaatca taggaattca aaagctccac gtttcttgaa aatgtggagc
     5221 ttttttttcg cgtctatgtt ttcattcgtc tgcgcaaaga tctgatcgca attaagccga
     5281 aacagagagc gggaaatact tatccttgag gtcgttgata ggtttttcaa agacctgata
     5341 actaaggctt gcaaccacgg cagttaccac aaaagaaatt ccatacaaga ccatattccc
     5401 cgaatcaatg gaacccaatt tgagaaccag ccaaatggca gccgtgtgat acacgtatag
     5461 cccatacgat atctttccaa gataccgcag cgggggcagg tcgagggtat ggttgaaaag
     5521 tcctgtcttc gccacgttat agatcaaaac cataaacagg taattcaaca acgagtatcc
     5581 ccagatctgc atgtaaccaa ctgagaatag aaatccctgg ccgagggtac tccaaagcgc
     5641 acgaccttcc acaaggtatt gagtcgacag gccgattgca gggacaagga tcgaagcaag
     5701 aaacagttga agccgcggac ggggaatctc aaactgggag gcaaaggccc caaacgcaaa
     5761 ggcatcaata tgcgaaaaag gcatggcata aacggcaata tcgggaatcg tcgtcatgaa
     5821 aggcagccta tgataactgt agacccaata cacaactccg cgcatgatcg gtcccaatgc
     5881 aattgcgcca aagaacaacc gcttcatttt ctcattgggt gtcagaaaca cgatcaaggg
     5941 ccacaggata tagaactgct cctcaacaga aagcgaccaa aggtgggtaa agaaacgcga
     6001 atgttcgtag gcgctggtgg tatggaagaa gtcatagaca taaaaaatgg aaagccatac
     6061 ctggagccag aatttatcgc cgaactcatt caaaagcggc ttgaattcca aaacaggtgc
     6121 ggcaaacttc aagccaaaca tgaggaagat atagaaataa tacaacggca caatgcgtaa
     6181 aaaacgcctg ccatagaact tggtgaaata ctcccgtttc cccagagaat tcttcatccg
     6241 tatcaaaatc cctgtgatga gaaaacccga aagtacaaaa aataactgca accccaccca
     6301 gccaaagctc aaattatcag tatgagcacc aagaaccaaa agaaaagcaa ccgcccgcaa
     6361 gccatcgaga cctggaatca taagcgtgat tatacgtgct gaaattcaag gctacaagaa
     6421 gtttttacgg accccagcgg ggctgataat cgcaataatc gttatctgtc aagcggcgca
     6481 gatctgtacc atccactcgc atgatgtaga tctcacagcc gtggacttcg aaacggtcaa
     6541 agtaggcagt aaacgccacc cattcgccat caggagaaaa ggaaggtccc tgcgaatttc
     6601 cgccagcggg tgaaatctgc tgcaggtcag agccatcagg attcatggaa aataattccc
     6661 gttcccaggg ttttcctgaa tacgtgacga tcagccctgc cgcagaccag tcacttcgtc
     6721 cacgcagggc aggtaaatga ctcacctggc gcaacccact gccatcgagg ttgatgataa
     6781 atagctgact taagccatca gcataggaag cgaacaggat ctgcgtccca tctggcgacc
     6841 aggttgggtc ccatgcacgc acgtccgata cctgccgcgg gttgccgcca tctcgatcca
     6901 tcaaccaggt ggtggggtgt tcaggatcgt caaagaagcg tgtaaaagca atcgaatttc
     6961 catcgggcga aatttcaggc gcgttcaggt cgccaagagc aaaagtcagg gctttgggtt
     7021 cgcccgcagc cagatcgaat tcatagatct cataatgttt gctctgttca tccagggctg
     7081 aatacaatac actgagcccg tcaggtgaaa tggatggata aaagtggcgg gtgtcatctt
     7141 cggtggtcag gcgctgatag ttcgtcccat cggcattgac catgcagatc tgttcccgtt
     7201 caagctcttt gaagacctga caggtaaagg caattttccc tgttggttcg gaaggctctt
     7261 caaccactgg agcagttggc gctgctgtat caacggcaac tggcgtactg gcctgcatgg
     7321 tttgaaacaa atgcggggtc ggcacagggg ttgccactgg cagattgcaa gccgccacaa
     7381 gaaataaaat tgaaaggtgg atcaaggaaa gttttctcat attcaagctt tgtaaacggc
     7441 attgatcttt ctctaacaaa actcttactg acaagtctgt ccgtacgata taaaatttta
     7501 cacacaacca agatcatcaa tccgcatcat tttgcgggtg aaattcaatc aacactcagg
     7561 aggcaaccat ggcaaacgat gcacctcagc aatacacttt caaggctgaa acaaaacaat
     7621 tactgaacat cctcatccat tcattgtaca aggatcggga ggtttttctg cgggaactgc
     7681 tctcgaacgc atccgacgcg cttaatcgcc tgcgctttga aatggtcacc aaccaaaaca
     7741 tcctcgactc aaacgcggag ctgcaaatcc gcatcaagac cgacaaggat gcacgaatgc
     7801 tcatcattca agacacgggc atcggcatga ccaaagacga ggtcatcgaa aatctgggga
     7861 ccatcgccca atccggtgcg cgcaagtttt tggatgcaac caaagacaaa aaagtggact
     7921 ttacacaggt cattggccaa tttggtgttg gcttttattc cgtattcatg gtggcagaat
     7981 gggtacgggt cacctcccgc tcttttcggc cagatgccaa tgcagttagc tggtatgcca
     8041 caggtgagga caactacgaa gtcagcgaag cagatatgag cgaaagagga acgaggatcg
     8101 agatcaagct caaggaagat gctgccgaat tcgcagagga attccgactt aagaacatca
     8161 tccataaaca ctcggattat atcggcttcc ccatctacat gggcgacggc actgaagcag
     8221 tcaataagca aacctcgctc tggcgcactt cgaggcagga gatcaaggaa gatcaataca
     8281 aagagtttta ccgccaaacc acacttgatt tcgaagaccc gcttttgcat acgcacatgg
     8341 tcactgatgc gccagtgcag ttgtatgcac tcctgttcat ccccgccaag gccgaacgcg
     8401 gaattttcgc actgcgaaag gaagacggcc tgaaacttta ctcgcgcaac attctgatag
     8461 atgactacaa caaagacctg ctgccagaat atctgcgttt cgttcaaggt gtggtggact
     8521 cggaagacct gccgctcaat gtctcccgtg aaactgttca atcaagtggg ctgatgccga
     8581 agctgaaaaa agtcctgaca aaccaggtca tgcgcgaatt ggagttgctg gcaaaaaaca
     8641 acgcagaaaa atatcacaca ttctggcagg agtttggtcc atacttgaaa cagggtgtgg
     8701 ccgccaacct cgcggaagca gatgccatca agccgttgtt acgcttcaaa accaatctaa
     8761 accccgaaac atggacttcg ctcgaagagt acgcatctcg aatgaaaccc gaacaaaagg
     8821 aaatctacta catcatcggc gaagacccga aatctgtgct gcgcagtccg caccttgatt
     8881 atttccattc acagggaacc gaagtgctgc tgctcacaga cccgatggat tccttcatgt
     8941 taatgggact gcgcaaatac aaagaccttg atctgaagaa tgtcgcccag gccgatgtag
     9001 acaccgcaaa acaatcaacc gaacaacccg atacggaaaa aatctcaggc gatgaattca
     9061 acaccttgat cgaacggttc aagcaggtgc ttggcgaccg cgtcacagat gtacgtgcca
     9121 gcacccgcct tgttcaatct gttgcacgcc ttgtggatac agatgccaac gcaaacccgg
     9181 atatgcagcg tgtgtacaaa tacctcggca aggaatatga aattccaaaa aaggttttgg
     9241 agttgaatcc atcccacacc atattgaaga atttgcttca tcaggaatca gattcagaat
     9301 tgcaaaacat catcattgaa cagatctatg atagtgccct gctggtggaa ggtctccacc
     9361 ccgacccatc cagtattgca gagcgagtac agcaaatcat ggaagcagca ctaaaaaaat
     9421 aacggcacac atacgcaact aaaaagataa aaactgtcgc tatttgtgta aatcggttgt
     9481 aaagcattga tattgtgatt tttattacaa tatcaatgct tttattttta aacctgaaaa
     9541 gaaatacatt ttgttaccaa attgtgacaa ggctgtgaca tccccgtaac aatggggcgc
     9601 tataaataaa gtatggaaaa caacaaacaa aaaaggagac aatgaaaatg aaaaagcaaa
     9661 ccaccctctt ctctgtgcta ctcgtcattc tggctttgtt tttctcaaac acccagccag
     9721 ttggcgcggc tgcaccagcc gtgatctcag ccgctctgcc caaccctggc gcgatcattc
     9781 cagttgccgc tggaaaaacc gccacgtact cacagggacc acacacttcg ggcttcccat
     9841 ccagctgggg cattgtaaac cctggtaaag tcaccagcag cattgacctt ggctactccg
     9901 gcaccgttgt tgctccgatc agtggatacc tcgccatcat gaaagattgc ggcgatcacc
     9961 aagtggcagt tgtccgcgcc tcaaatggct gggccatcgg cctgacacac attttcgtca
    10021 acaccacagt aagaaacaaa tcagtgtcac aggggcaggt gatcggcaaa accgttccgc
    10081 ctcctgcaac cagcaaaggc tgcggatacg gaaaaggctc acatatccac tacacgctaa
    10141 tgaaatgggt ccagagcggc acgatcaaat acactgaaca agacattgtc aacacctatt
    10201 tttccaagtg gatcgtgaaa aacacctatc ttgatggtcc caccaggatc cccattggcg
    10261 gtgtgatcaa ataaaaagcg caaactttag caaagcaggc atccatatac aggatgcctg
    10321 ttttgctctt aaaacaaact acaatgtgga caaaccaaat ttacaggaac cgaaataatc
    10381 aaatggatat tcaaaccgca tacaacgaat ggtcaggaat ctacgacaca aacaccaacc
    10441 tgacccgtga tctcgattca aaactgaccc gtgacctgct tgcccatcaa aaatttgact
    10501 ccatcctcga gcttggctgc ggaacaggca aaaacaccat cttcttcagc caaattggga
    10561 agaaagtaca cgcactggac ttctcacctg gcatgatcga aaaagccaga acaaaggtca
    10621 gcgcaagtca tgtccgcttt gaaatggcag acctcaccaa gcactggcca tgtcaaaacg
    10681 aagcctatga cctgatttcc tgcaatctgg ttttggaaca tatcgctgaa ctcccccata
    10741 ttttttccga agcagcacgc gtgttgcatc ggggcggaaa attcctcatc aacgagctac
    10801 atcccttcaa acagtatcag ggcacaaaag ctcgtttcga gcgcggcact gagaccgttg
    10861 aactggaagt ctttgtacat cacatctctg aattcatcac caccgccctg aaacatgatt
    10921 tcaagctact ccacttcaat gagtactggc atccgcaaga tgaaaacaaa ccgcccagaa
    10981 tcgcaacttt tattttcgaa aaacagaact aagggcaaag tctcaaggaa gtaatttttg
    11041 cagcctgtcc acataagcgg aaagcaattt cataaaatac ttccgcctcc aggtggcgtg
    11101 gctctgaata aactgatgca gccatccatg ctcgttccca tgttcatgtt ccgcctcttc
    11161 gtctatctgc catttatcca gaaccgcata aatgcaatag agattgatcg ccacctcatc
    11221 aggctgctca gacccaaaat acccctgcaa tatggatcga tggaacttct gcatcacttt
    11281 ctcgcggata aacaaaccat aggacagagt ttgcaggcga taggtctgca aatcggtcat
    11341 gatcttggca atatccatgt aaatggggcc atcaatgaaa tgcgggtcca atatcccaat
    11401 acgattgtct tcggcaacta aaaagtttgc acagtcaaaa ttccaatgca acaaaccaaa
    11461 agacaagttg tgatccttgt aagcggctct tgtttgcctc aaaacattac ggacaagtgt
    11521 aaagtcaaag tcatggaatg gctcaatgac atttatgctc ttctcaagag cggcatggat
    11581 atcatcgcta aaaaacggtc cttcgtgcaa cttacccaac tgctcatgat acacacgcaa
    11641 ccagcgtccc gaacgataaa accagttact taattgttcc tgccagcctg catggtaggc
    11701 cgttttgaaa ttcaatatca tgttgcgcaa tggacgtgct tccagttctt ccatgacaag
    11761 ggcgctgaag gggtcaaaaa atgccaatgg acgaactgcg caaaagtggt ctttttcttc
    11821 actactaaaa tatccgtatg gattggtgtt gttattttcc cccgggatat tctcagccgt
    11881 tgaaacaaaa ttctcaatgg ctttcagagc ctcataatca ttccgtcctt cctctttcaa
    11941 accagggctg ctaatcgatt ctgaaatact ttgatcaggg tggcggcgaa ttttcaccag
    12001 aatcgcccgc ctctgtaaat ctgccagttg aatttggtaa cgcacaaaaa ccgaccattc
    12061 ccgcatccgc acatcagggg aacttatgat cgacgtacca ataccatacc aattagaaag
    12121 attcgctcgc agggttaaag tcagatctgg aagcaaaact gcaacttcgt caaaagacac
    12181 aaaacgctcc tttaaaaacc aataaattaa aacaaagcta catcaggtct ctggttttat
    12241 tgtatcagca ggaaaagaac accctgcgct acttgcattt caacaatata ttaattattt
    12301 tgattccttt tcgtccactt tgtagtgtca tttatagtag catggtgaac ttgggaaaca
    12361 cactccacca ttagtccttg ccaatttgga tttttttacc agatgcccac ctttagatac
    12421 caatgtcacc gcccagattt ctgaaaatct ccccgcaacc tgcaaggatt tgttatttgt
    12481 atttccgagt aagtagtgtg tcaaccattg tccctaccag cagcccaagc gctgtgaaaa
    12541 agaccttgaa agcaaggact ctgtcaggaa agctccaggg cttttgccag atccataatt
    12601 tcttatcctc tgtcagagct agcgagaccc tccagggact tgcaatcgta ctgatcgaac
    12661 tgccttgagg agtctcagca aatatggtgg aagtgacaca ggatttgatc tcctgttgaa
    12721 acaagggata taaaaaataa tcactttcgc atcggccttc actgaccaca tacgcatcac
    12781 ttggaacaag gtctgaaacc gcctcatcct cctgccaaca acctgccgcc tcctggcatg
    12841 gatacaaaat atgataagtg gcgccatctg tggtcgtcac ccaaaactca ttgccatagg
    12901 gttgtaaatt gagaatcgtt tctacgggat ggggaaaata atcgatcggc tgccaaaacg
    12961 tccccccgcc cgcaaaccaa aaactggtta acaagtatcc gaacaatggg cccaaggtga
    13021 tgcaagccag aaatattttc agtctttttg aagattccat aaggcggata ttttatctca
    13081 aacgaacacg ggggttgctt tttcaataaa aacgggacag aaaatagcag gaactttctg
    13141 tcccgttgta gaagtgaccg ggctaaaacc cctccgccac gtactcaccc cacacgcctc
    13201 tcagtgcatt gcaaacctca ccgagggtaa tatcattttc cacgcactca ataaagagcg
    13261 gcatcaaatt ttccgtccct ttggcagctt cggttaattt tccaagcaat gctgtaaccc
    13321 gcccactgtc acgtgcatca cgcaatttct tcaacttctc ccgttgaccc agttcaatgg
    13381 acgggtccac tttcaagcgt tccagagtga gaacttcatc cacggaaaat tgattgacac
    13441 ccacaacgat ctgctctttg cgctcgacgg cttgctgggc ggcataggca gcattttgaa
    13501 tttcattctg catgtaaccg cgctcaatgg attgcaatgc gccgcccatt tcgtcgattt
    13561 tggcaatata gtcaagcgca cctttttcga tttcatcggt caaatgctcg atcaggtatg
    13621 aaccagccag cgggtcgatc gagtcggcta cccctgattc gtaggcaatg atctgctgtg
    13681 tacgcaatgc cacgcggaca gatttctcgg ttggcagcca caacgcctca tccatgctgt
    13741 tggtgtgcag gctttgtgtg ccgccaagta ctgcagaaag tgcctgcagg gtcacacgca
    13801 ccacattgtt ttcaggttgc tgcgccgtga gtgtggagcc tgcggtttga gtatgaaagc
    13861 gcaactgcca cgaagcaggc ttctgtgctt taaatctctc gcgcataatt cgcgcccaca
    13921 tgcgccgtgc tgcgcggaat ttggcaacct cttcgatcag gttgttgtga gcattgaaga
    13981 agaatgaaag ctggccagca aaatcgtcca cattcaaacc agccttgagg gcggcttcca
    14041 cataggccat gccattcgcc agcgtgaaag ccacttcctg cacagcagtg gaccctgcct
    14101 cacggatatg gtaccccgag atcgagatcg tattccaaaa tggaacttca gcggcgcaaa
    14161 attggaagat atctgtgatc aaacgcatcg aaggtgcagg cgggaaaata tacgttccac
    14221 gcgccacgta ttctttgaga atatcattct gaatcgtgcc gcgcagctgg cgcatatctg
    14281 caccctgacg ttttgccacg gcgatataca tggccagtaa aacacctgca ggcgcattga
    14341 tggtcattga tgtggaaact ttttcaaggg ggatctggtc gaacaactgc tccatatcac
    14401 ggatggaaga aatggaaaca cccaccttgc caacctcacc ctgtgcgatc gggtcatcgg
    14461 catcgtaacc gatctgggtc ggcaaatcga aggccacact cagccccgtc tgcccctgtg
    14521 caagcaggta gcgataccgc ttgttggatt cttctgcagt ggaaaagcca gcgtactggc
    14581 gcatcgtcca aaagcgtgaa cggtacatcg tcggttgaac gccgcgggtg tagggatatt
    14641 ctccggggaa
//
LOCUS       DAOQLC010000106        14418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1528_length_14418_cov_24.068022, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000106 DAOQLC010000000
VERSION     DAOQLC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_14418_cov_24.068022"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..116)
                     /locus_tag="PL000_16120"
     tRNA            complement(43..116)
                     /locus_tag="PL000_16120"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Asp,seq:gtc)
     gene            complement(161..1297)
                     /locus_tag="PL000_16125"
     CDS             complement(161..1297)
                     /locus_tag="PL000_16125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC90460.1"
                     /translation="MKRITTILFIASLFLATTQSVHAHKQTPPSEPESGGVVCEPAAY
                     LVPPDDCLPIGPSTYLTNLAHLGITFPPRPLPASKPDLSLTQLPYFYFHLDKDYVPVY
                     GAPGEKGPGGQQFPPGFVYVSYIDRVEQNGVYYLMPNGGWIPGKGSRVGEISNFQGLI
                     FSSTPKNSFGWAFEMLPVMKSPGYNAAQTGKQIQPFEVVIVYDVQTMDNEEWYMIGPD
                     QWLEGRKVARVIVNTTIPQGVTSNRWIDVDLAEQTLAVYENNRLIFATVIASGLDPFW
                     TRPGLFQIFEKKETETMRNNDMADFYYLDNVPWTMYFDGARALHGAYWRTRFGYPQSH
                     GCVNLSVGDSHWLFNWAVVGDWVYIHDRTGQTPTDPALYTGGAY"
     gene            1452..1823
                     /locus_tag="PL000_16130"
     CDS             1452..1823
                     /locus_tag="PL000_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HNC90461.1"
                     /translation="MSKNRSQQMVDRLRELQASSPDIEASAVVSVDGLSIASALPQGV
                     EEDRVSAMSAAMLSLGERIASELGRGSLEQVYIKGLKGYVVLMSVGDEAVLTALAREQ
                     AKLGLIFLDMRRAAEDLAKLI"
     gene            1834..2469
                     /locus_tag="PL000_16135"
     CDS             1834..2469
                     /locus_tag="PL000_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-vinyl reductase"
                     /protein_id="HNC90462.1"
                     /translation="MAPLQKSGLFYPNKFGMITIKSLEEVMGKNGLNAILNLAGLNNY
                     IENYPPDNLDKGFDFAELSAIGSALEEMYGPRGGRGLALRAGRATFSDALRNFGALAG
                     AADLAFVVLPLQSKLRIGLPAFAKIFSQLSDQQTTVEEKDTEWIWTIHKCPCCWGRTG
                     ADKPVCFIAAGLLQESLKWVSGGNEFRVNESKCVAIGDSVCEFIVQKEPIS"
     gene            2488..3342
                     /locus_tag="PL000_16140"
     CDS             2488..3342
                     /locus_tag="PL000_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90463.1"
                     /translation="MADIKSKILIIEDDLDVAEMLNAYFRVQGYDVFTVNWGEDGVRS
                     CQTIHPDLVILDIRLPDIDGYEVARRLRNDRRTSEVPIIFLTEKRDRSDRLQGLEIGA
                     DDYITKPFDVQELRLRVRNALKRVSQGSLTNPVTGLPEGALVDEKLSEVIGKDGVAVL
                     FVSIDNMDSFREAYGFVASDDVLRAISLMIVNTMRETSRPEDFLGHLSTTDFILVIPP
                     SNLAALSEKLRARLEQSVEYFYPIKDREQMVNSRNRLGVKLAEIASLKDRFSDVGQIK
                     AELVRLKK"
     gene            3351..4091
                     /locus_tag="PL000_16145"
     CDS             3351..4091
                     /locus_tag="PL000_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNC90464.1"
                     /translation="MRITVVTPTYNEAENLPKLVSALFSLPLDINLLVVDDNSPDGTG
                     RIADELVVKYPGRVDVLHRPGKMGLRSAYLNGFQKVMDSSVDAIVQMDADFSHDPSAL
                     LDMANLLTDNDIVLGSRYVKGGSVDERWPLWRRSLSAFGNYYARTILGLPLHDVTTGY
                     RMWRRETLQQVPFERIHSSGYVFLVEMAYLAHCLEFKIGEAPIYFADRRWGKSKMSIK
                     IQLEAAIRIWQVWWNYRDLKKAGKAGRL"
     gene            complement(4094..5878)
                     /locus_tag="PL000_16150"
     CDS             complement(4094..5878)
                     /locus_tag="PL000_16150"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC90465.1"
                     /translation="MGNPYYITHIIILLISSTIGILLLLRVWSLRSLPGAHGLILTIL
                     CVVEWSLAYAMEIVRLDLTEKVLWGKLQYFGISYAALGMFIFAVNYSGHGARLTSTRK
                     ASLALLASLGFIAALTNDRHHLIWNQIQFTNNIPFGPLELSHGVLFFVVVAFQYALLL
                     AVTGIFLQIAIHGQSLYRAQSKIMLVGMSFPWLANLIYVTGWNPYKGVDFTPIALTFS
                     AIAFSISFLRYRFMDLLPVAHDSVFNAMKDGVIVLDSKDRIADINPMGSFIFQNRGNL
                     LGNEIRVLFPDWNNWISPGHHDEIHQELCLDYNGETATFKLRITPILEQQGEHNGNII
                     ILSNITEQKRFQEQIMEASRLKSQLLANLGHDLRSPLGAIIGYAEMLEDGSFGPMSNE
                     QVKATAEILDGANQLLSFINNLVGQAQIETGKVILREYPFNVEEVIGPLLSTLNFHAH
                     KKGLILIQFVDPDLPKKLVGDQFWLRQIVMNLVHNAVKFTDQGSVTVRFIVQGEDQWA
                     IQVSDTGIGIPPEAQKRVFQAFEQINSMENAKQSGFGLGLSIVAKLTSLMNGKIELKS
                     EAGTGSTFTVILPLKEAVEQEGPTMFVR"
     gene            6136..7992
                     /gene="typA"
                     /locus_tag="PL000_16155"
     CDS             6136..7992
                     /gene="typA"
                     /locus_tag="PL000_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNC90466.1"
                     /translation="MLIERTDIRNIAIIAHVDHGKTTLVDGLLGQSHTFRENQHVEER
                     VMDSNDLERERGITILAKNTAISLIDENGKTVKVNIVDTPGHADFGGEVERVMNMVDG
                     VLLLVDAAEGPMPQTRFVLKKALELGHKAVVVINKVDRKDAEPARVLNETFDLFIELG
                     ASEEQADFPVVYAQATAGRAGLTPEPGPDLKPLFDVILKHIPAPKVDVDAPLQLLVTM
                     LGYDDYRGVTAIGRVFAGTIKAGQKLARMKLDGSILPESARYLYSFKGLNKIEIDEVK
                     AGDIISLAGLEGIAIGETLADPANPVALPTIKVEEPTVRMTFGVNTSPFTGREGTWST
                     SRKLRERLYDELRTNVSLRVEDTESAENFLVSGRGELHLGILIETMRREGYEFQVSRP
                     EVIYHKADDGVLLEPYEEVHVETSNETVGVVVEMLGSRRGKMMEMHDAGQGMTRIVYV
                     VPTRGLLGFRYQFLTSTRGEGVMHTIFSGYDEMAGPISSRTKGSIVAWEAGSTTAYAL
                     KAAEERGQLFVGPGVEVYEGMVVGENARGGDLSVNVCKKKHLTNMRASGGDMEIRLTP
                     PRQMSLDEAIEYLADDELLEVTPENYRIRKRILSTDERGKQTKKAKEQFGEE"
     gene            complement(8041..8781)
                     /locus_tag="PL000_16160"
     CDS             complement(8041..8781)
                     /locus_tag="PL000_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC90467.1"
                     /translation="MKKFMGLCLVAGIFISACSMPSAAGPSDSEISTAAALTVQAVIN
                     STPLASPTAAGGQQLDPEAATPVVITPTFSQPMASVGDVINCRSGPGTNFERVTQILP
                     TDSVKIVGFYPPNYWVVNTKDGDCWISGEFTTPVGSFAAVPTVTAPPTPEGGGPEAAT
                     FPKNGWTFFCYGPGKADITLSWNDNAENELGYRIIRDGEKVAELAPNTKYFAETIDLN
                     SGESVAYQIQAYNEAGSSDSATANITCP"
     gene            complement(8793..10163)
                     /locus_tag="PL000_16165"
     CDS             complement(8793..10163)
                     /locus_tag="PL000_16165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC90468.1"
                     /translation="MMNLSTAHIKDKNFRKLSQKVVAEMKRLSIPGVAIGVWHKGREF
                     SDGFGITSIENPLPVTSDTLFQVGSISKTFTGTLIMQLAEQGKVNLDAPVHTYLKDLK
                     LSDKAVEKQVTVRQLLTHTGGWVGDYFNDFGNGDDALARMVKDIAHLPQVMPLGEIWS
                     YNNTGFNIASRLIEVLTGKPYEQAAQEMLLDPLGLKMSFFFPSDLLITHRFVTGHYNE
                     NKKTFVSRPWAIGRAGNGVGGVVSTVKDLLAYARFHMGNGSRIIKRKSLEAMRMPQIS
                     AGGRGEMGITWFINRSGSLTRYSHGGATNGQQALFVFIPEKDFALALLTNNDNGGVLN
                     NNLLGLALEVYFGIKPKLPKPIKVSTNELKEYVGSYRIGTEAFDLKLKNGELIFQHIP
                     LGGFPTPDSPPGPAMPPMRVAFYEKDQTLMLDEPMKNGIGDFIRDKNGRVQFFRIGGR
                     AHKKIK"
     gene            complement(10163..11326)
                     /locus_tag="PL000_16170"
     CDS             complement(10163..11326)
                     /locus_tag="PL000_16170"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HNC90469.1"
                     /translation="MPFYHKLGKIPHKRHTQFKKADGKLYREEVMGLEGFSSIQSILY
                     HHFLPPRVKMTEDLGSAKPEYVDFGPIRHRAFRTDQTPLGPDPVSARIPLLGNNDVIL
                     GVSRAKDSMGYFYRNSQAYETWWVHDGSGTLKSQFGNLPFRKGDYIVIPFGVTWQMQL
                     NEECKFFTIENPSQIEPPKRYRNNYGQLLEHAPYCERDIRLPEELETHTERGDFEVRV
                     KVRDRLSRHILDFHPFDVVGWDGYLYPWIFNIEDFEPITGRLHMPPPIHQTFDAHNFV
                     VCSFVPRLFDYHPEGIPAPYNHSNIQSDEVLYYAEGNFMSRKGIDRSDISLHPFGLPH
                     GPQPGMTEASIGKVETKELAVMVDTFHPLNLTKQAAAMEKPEYMSTWLEGDGE"
     gene            complement(11357..11833)
                     /locus_tag="PL000_16175"
     CDS             complement(11357..11833)
                     /locus_tag="PL000_16175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC90470.1"
                     /translation="MRNTHPRDEDTYTSDVFEVLFNYEVTRSRRYPSPLALLYIEMAP
                     ITLDAELQAQAPAVFAAALNSHLRSVDIPSTYGRAYRVLLPSSNEHGARAVCERLLSV
                     YRNKFEVANGSIAFSLHIGATAHNGGAELSAAELIQKAEEALKQAKLKGPNTYMVI"
     gene            complement(11855..12250)
                     /locus_tag="PL000_16180"
     CDS             complement(11855..12250)
                     /locus_tag="PL000_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC90471.1"
                     /translation="MEQNKIITVRPKETFISKQQLPNFEGISTQTAGTKHLCMHLVII
                     PPGGRAVAHYHNGYETTIYIIKGRAETRYGEKLEHSSINEAGDFLFIPPNLPHQPINL
                     SETEEVVAVVARNDPNEQESVVVYQPQDS"
     gene            complement(12266..13159)
                     /locus_tag="PL000_16185"
     CDS             complement(12266..13159)
                     /locus_tag="PL000_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC90472.1"
                     /translation="MIFATCRTPHTAPRPALVSNDRIYPLPLADMHAVIALGADKAAS
                     LASQESFAPAEVQFLAPIQPASLRDAYAFEQHVRTASQNRGRDVPQEWYKFPAFYYTN
                     PRTIFGPDEVIPNPHYTSAMDFELEIAVIIGKSGRDIKPEDAPAHIFGYTIFNDWSAR
                     DVQREELRIGLGPAKGKDFASSLGPVIVTSEALADRAVDRPGVYDLMMTAKINGVEMS
                     RANLKDIFWSFGEIIARASDTCELQAGDVIGSGTVGTGCLLELTKGQGPWLNAGDVVE
                     LEIEKIGTLKNTIGTKPTPLF"
     gene            complement(13231..14361)
                     /gene="hppD"
                     /locus_tag="PL000_16190"
     CDS             complement(13231..14361)
                     /gene="hppD"
                     /locus_tag="PL000_16190"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HNC90473.1"
                     /translation="MATKTIDETRPMTEEADFLQIKSIDHVHFYVGNAKHAMYYWWKA
                     FGFKPVAYSGLETGNREFASYVLESGQARIVVSAPYGPSSPLASHHMVHGDGVKVIAM
                     EVDDVEKAWQATTSRGAKSAWAPREEKDDHGIYRTSSIYTYGETLHTFVDRADYKGVF
                     APTYRPIKDKEAHSTGLAAIDHIVGNVQLGKMNQWVNFYHHVMGFRQLMHFDDKDIST
                     EYSALMSKVMQNGNGRVKFPINEPAEGKRKSQIEEYLDYYLTPGAQHLAIITGDILTT
                     VDQLRKNGVEFLRVPDTYYEALPERVGSIKEDYKTIQELGILVDKDDEGYLLQIFTRP
                     IQDRPTMFIEIIQRHGAQGFGKGNFKALFESLELEQQRRGNL"
BASE COUNT         3411 a         3353 c         3857 g         3797 t
ORIGIN      
        1 gtctttttgt tgctgtgaat acagcatttg ccttcaataa ggcggtcccg acgggattcg
       61 aacccgcgat ctcggccttg acagggccgc atgttaggcc gctacaccac gggaccaatt
      121 agctaagcgg gcgatagttt accatgatgg tattttgatg tcaatacgct ccgccagtgt
      181 aaagtgcagg atctgtgggg gtctgtccgg tacggtcatg aatgtagacc caatctccaa
      241 caactgccca attgaataac caatgtgaat cgccaacgga taaattgaca cacccatgtg
      301 actgtgggta tccaaaacgg gtgcgccagt aagcgccatg aagtgctcgc gctccatcaa
      361 agtacatggt ccatggaacg ttgtcgaggt aatagaaatc tgccatgtca ttgtttcgca
      421 tggtttctgt ttcttttttt tcaaatattt gaaaaagacc ggggcgtgtc cagaatgggt
      481 caagtccgct ggcgatgacg gtggcaaaga tgagtcggtt gttttcgtat actgccagag
      541 tttgttcagc aagatcaaca tcgatccagc gatttgatgt gacgccctgc ggaattgtgg
      601 tgttgacgat gacgcgggcc accttccgac cttccagcca ttgatctggt ccgatcatgt
      661 accattcctc attatccatg gtttggacat cataaactat cacaacttca aagggttgga
      721 tttgtttgcc tgtttgggct gcattgtagc cgggcgattt catgacaggt aacatttcaa
      781 atgcccagcc aaaagagttt ttaggtgtgg aagaaaagat caagccttga aaattcgata
      841 tttcgccaac gcgtgagcct ttcccgggaa tccagccgcc gttgggcatg aggtaataaa
      901 cgccattttg ttccacacgg tcaatgtagg agacatatac aaaaccaggt ggaaattgtt
      961 gcccgccggg acctttttcg cctggggctc cataaactgg tacatagtct ttgtcgaggt
     1021 ggaaataaaa ataaggtaac tgagtcaggc tcaggtcagg ttttgaagcg gggagtggtc
     1081 tgggagggaa ggtaattcca aggtgggcaa ggttggttaa gtaggttgaa ggtccaatgg
     1141 ggaggcagtc atctggggga acgagatagg cggcgggttc acaaacaacc ccaccacttt
     1201 cgggttctga ggggggcgtt tgtttgtgcg catgaacaga ttgtgttgtt gcgaggaaaa
     1261 gagatgctat aaaaaggatt gtagtgattc ttttcatggc gggaaattat agcatgtgta
     1321 gtttgctgtt ttgtaagaat agaagttgcc gaacttgaca caatgcatcc catgttctaa
     1381 aatagtacaa ctattataaa gaagcttgca tcctttcaaa atggatgttc gcatccccac
     1441 aggagggctg gttgtccaaa aatcgttcac aacaaatggt tgatcgcctg cgtgagttgc
     1501 aggcttcgtc acccgatatt gaagcatcgg cagttgtaag tgttgatggt ttgagcattg
     1561 catctgccct tccgcaaggc gtagaagaag atcgtgtttc agccatgtct gcggccatgc
     1621 tttcattggg tgagcgtatt gcaagtgaat taggacgtgg ctccctcgag caggtttaca
     1681 tcaaaggctt gaaggggtat gttgttttga tgtctgtcgg tgatgaagct gtactcactg
     1741 cactggcacg cgaacaggcc aagcttggcc tgatcttcct tgatatgcga cgcgccgccg
     1801 aagatctggc gaagttgatc tagtggagta gttatggccc ccctgcaaaa aagcggttta
     1861 ttttatccca ataagttcgg tatgatcacc atcaaatctt tggaagaggt gatgggtaaa
     1921 aatggcttaa atgccatttt gaatttggca ggtttgaata attatattga aaactacccc
     1981 ccggataatc tggacaaggg ttttgacttt gctgaacttt ctgcaatcgg ctccgccttg
     2041 gaagaaatgt acggtccacg cggcggacgc ggtttagccc ttcgtgcagg gcgtgctaca
     2101 ttctcagatg ctttgcggaa ttttggtgca ttggctggtg ctgcagattt ggcctttgta
     2161 gtacttccgc ttcaatccaa actgagaatt ggtcttcccg cctttgcaaa gattttcagc
     2221 caattgagtg accagcaaac aacagtggaa gaaaaggata ctgagtggat atggaccatt
     2281 cataagtgtc cttgttgttg gggccgtacc ggagcagata aacccgtctg tttcattgca
     2341 gcaggtttgt tgcaggaaag cctcaaatgg gtttctggcg gaaatgaatt ccgcgtcaat
     2401 gaatccaaat gtgttgccat tggtgactct gtttgtgaat tcattgtcca aaaagagccg
     2461 atttcataac tcaagtgcag gtttggcgtg gcagacatta agtccaaaat tctcatcatt
     2521 gaagacgacc ttgacgtggc tgaaatgctg aacgcctatt ttcgcgtaca aggctatgat
     2581 gtttttactg ttaactgggg tgaggatggg gttcgctcct gtcaaaccat tcacccagat
     2641 cttgttattc tagatattcg tctcccggac attgacggtt atgaagtagc ccgtcgcctt
     2701 cggaatgacc gccgcacctc tgaagtcccc attattttcc tcacagaaaa acgagaccgc
     2761 tctgaccgcc tgcaagggtt ggaaatcggt gcggatgatt acattaccaa gcctttcgat
     2821 gtacaggaat tacgcttgcg cgtacgcaat gcgcttaagc gtgtcagcca gggttctttg
     2881 accaacccag tgactggcct gcctgagggt gcattggtgg atgaaaaact ctcggaagtg
     2941 attggaaaag acggggttgc cgtcctcttt gtttcaattg ataacatgga ttctttccgt
     3001 gaagcatatg ggtttgttgc ctctgatgat gtgttgcgtg caatcagctt gatgatcgta
     3061 aacacaatgc gggaaacgag ccgccccgag gatttcctgg gccatcttag tacaactgat
     3121 tttattttgg ttattccgcc ttcaaatttg gcagccttaa gtgaaaagct gcgagcccgc
     3181 cttgaacagt cggtggaata tttctacccg atcaaagacc gtgaacaaat ggtgaatagc
     3241 aggaacaggc ttggggtcaa attggctgag atcgcttcat taaaggatag attctcagat
     3301 gtgggtcaaa tcaaagcgga gcttgttcgg ttaaagaaat aggagtttgt ttgcgtatta
     3361 cagttgtaac acctacgtat aacgaagcgg agaacctgcc caaactggtc tccgctttat
     3421 tttccctacc acttgatatc aacttgttgg tcgtggatga taacagccct gatggaacag
     3481 gtcgtatcgc tgatgaattg gttgtgaaat atcccggccg tgttgatgtg ctgcatcgcc
     3541 ctggtaagat ggggttgcgt tccgcctatt taaatggctt ccaaaaagta atggacagta
     3601 gcgtagacgc catcgttcaa atggatgcgg acttttcgca tgacccgtcg gcgcttttgg
     3661 atatggcaaa cctcttaaca gacaatgata ttgttcttgg gtctcgttat gtaaagggtg
     3721 gttcagtgga tgagcgctgg ccgttatggc ggcggagcct gtcggctttt ggaaattatt
     3781 acgcacgcac gattctgggg ttgcctttgc atgatgtcac aaccgggtat cgtatgtggc
     3841 ggcgtgagac cctgcaacaa gtcccgtttg aacggattca ttccagtgga tatgtatttc
     3901 tggtggagat ggcctatctt gcacattgtc ttgagtttaa gatcggtgaa gcccccattt
     3961 attttgccga ccgacgctgg ggaaaatcga aaatgtcaat taagatccaa ttggaagccg
     4021 caattcgtat ctggcaggtg tggtggaatt atcgtgacct gaaaaaagct ggcaaggcgg
     4081 gaaggctgta gaactaacgg acgaacattg tcgggccttc ctgttccacg gcttctttaa
     4141 gcggaaggat gacagtgaat gtgcttccag tgcctgcttc actcttcaat tcgatctttc
     4201 cattcatgag ggatgttaat tttgcaacaa tggaaagtcc caatccaaac ccgctttgtt
     4261 ttgcgttttc catgctgttg atttgttcga aggcttgaaa gacacgcttt tgcgcctccg
     4321 gggggattcc gatgcctgta tccgatacct gtatggccca ctgatcttca ccttgtacga
     4381 taaagcggac ggtcacagaa ccttgatctg taaatttgac tgcattgtgt accaggttca
     4441 tgacgatctg tctcaaccag aattggtcac cgacaagttt tttgggaaga tcagggtcga
     4501 cgaattgaat gaggataagt ccttttttgt gagcatggaa attcaatgtg gaaagaagcg
     4561 gtccaatgac ttcttccacg ttgaaaggat attcccgtaa gatgactttg cctgtttcaa
     4621 tttgagcctg accgacaaga ttgttgataa aagacagcag ttgatttgcg ccatcgagga
     4681 tttcagcggt ggctttcact tgttcgttgc tcatcgggcc aaaagaacca tcttctaaca
     4741 tttcagcata tccgatgatt gcaccaagcg gtgagcgcag gtcgtggcca aggttggcga
     4801 gtaactgact cttaaggcgg ctggcctcca tgatctgttc ctggaacctt ttttgttcgg
     4861 taatattgct gaggataata atattcccgt tatgctcgcc ttgttgttcg aggatcggtg
     4921 tgattcgcaa cttaaaggtt gcggtttcac cgttatagtc caggcaaagt tcctggtgga
     4981 tttcatcgtg gtgtcccggg cttatccagt tgttccaatc tgggaatagc accctgattt
     5041 cattccccaa taagtttcca cgattttgga aaatgaaact tcccatcgga ttaatatctg
     5101 cgatacggtc tttggagtcc agtacaatga ccccgtcttt catggcatta aacacagaat
     5161 cgtgtgcaac gggtagaagg tccatgaacc gatagcggag gaaactgatg gaaaatgcaa
     5221 tggctgagaa ggtaagggcg atgggagtga agtccacccc tttgtatgga ttccacccgg
     5281 ttacataaat aaggtttgcc agccacggaa atgacatgcc aacaagcatt attttagact
     5341 gtgctcgata aaggctttgc ccatgtattg cgatctgtaa aaatatccct gtgacagcca
     5401 acagcaaagc gtattgaaag gcgaccacaa caaagaacaa taccccatgg cttaattcca
     5461 gagggccaaa gggaatattg ttggtgaact ggatttgatt ccagatcaag tgatgacggt
     5521 cattggtgag ggcggcaata aacccaaggc ttgcaagtag cgccaacgaa gcttttcggg
     5581 ttgaagtaag ccgtgcccca tgcccgctgt aattgactgc aaaaatgaac ataccgagtg
     5641 cggcgtagga aattccaaaa tattgcagct tgccccaaag gaccttttcg gtcaggtcta
     5701 ggcgcacaat ttccatcgcg tacgctaaag accattccac cacgcataaa atggttaaga
     5761 tcaggccatg agcgccaggc agggagcgga ggctccaaac ccgaagcagc aaaagaatcc
     5821 cgatcgtact gctaatcagc aggatgatga tatgcgtgat gtagtatgga tttcccataa
     5881 gtaggccaat tttactgggt aaatgccagt tccgtgcgtt tcaagttaat tagaatatta
     5941 aacgctttgt tctggatggg tatctgcccg gaaatgacag gaatctgcct cgccctaccc
     6001 aaaaacatca caatctgata atatacacac cagcacgccg aaccactgat ttcaagccct
     6061 cggcctcccc gtcgggggtt tttgtgtgtc ttacggcctt tcataaaaca acgcccaaaa
     6121 gattaaagag accctatgct tatcgaacga actgacatcc gtaacatcgc catcattgcc
     6181 cacgttgacc acggcaaaac cacccttgtg gatggattgc tcggacaatc acatacattc
     6241 cgcgagaacc agcatgttga agaacgcgta atggattcga acgatcttga gcgtgaacga
     6301 ggcattacga ttcttgccaa gaacacagct atttcattga tcgatgaaaa tggcaagaca
     6361 gtcaaagtta atattgtaga tacgcccgga catgccgatt ttggcggtga agttgaacgc
     6421 gtaatgaaca tggtcgatgg tgtgcttttg cttgtagatg cggcagaagg ccccatgccc
     6481 caaacccgct ttgtgttgaa gaaggcgctc gagttgggcc acaaggccgt tgtggtcatc
     6541 aataaagtag accgtaaaga cgcggaacct gcccgtgtgc tcaatgaaac ctttgacttg
     6601 ttcattgaac ttggcgccag tgaggaacag gcggattttc ccgttgttta tgcccaggcc
     6661 accgcaggaa gagcgggtct tacccctgaa cccgggcctg atctgaaacc cctgttcgat
     6721 gtgatcctca agcatatccc cgcacccaag gtggatgtgg atgctccttt acagctgctc
     6781 gtcaccatgc tgggatatga cgactatcgc ggcgtcactg ccatcggccg tgtctttgca
     6841 ggtacgatca aagctggcca aaaactggcc cgcatgaaat tggatggctc catccttcct
     6901 gaaagcgcgc gttaccttta ctcattcaaa ggtttgaaca agatcgaaat tgatgaagta
     6961 aaggcgggcg atatcatttc gctggcaggc ctcgaaggga tcgcaattgg tgaaaccctt
     7021 gccgaccctg ccaaccccgt tgctctgcca accatcaaag tggaagagcc gacggttcgc
     7081 atgacttttg gtgtgaacac ctcgcccttt actggcaggg aaggcacctg gagtacttcg
     7141 cgtaaattgc gtgagcgttt gtatgacgaa ctgcgtacca atgtttcctt gcgtgttgaa
     7201 gatactgaat cggccgagaa tttcctcgtt tctggtcgtg gagagttgca tcttggcatc
     7261 ctcatcgaaa ccatgcgccg cgaaggatac gaattccagg tctctcgccc tgaagtgatc
     7321 tatcacaaag ccgatgacgg cgttttgctt gagccgtatg aagaagtaca cgtagagacc
     7381 agcaacgaaa ctgtgggtgt ggttgtggaa atgcttggca gccgaagagg caaaatgatg
     7441 gagatgcatg acgcaggtca gggtatgacc cgtatcgtgt acgttgtccc cacccgtggt
     7501 ttgctgggct tccgttatca attcctcact tccacccgcg gagaaggtgt catgcacacc
     7561 attttcagcg gctacgatga aatggctggc cccatctctt caagaacaaa aggctccatt
     7621 gtggcttggg aagcgggctc aaccacggcg tatgcattga aagctgccga agagcgcgga
     7681 cagttgttcg ttggccctgg tgttgaagtg tatgaaggta tggttgtggg tgaaaacgcc
     7741 cgtggtggtg acctttctgt aaacgtctgc aagaagaagc atttgacaaa tatgcgtgct
     7801 tcgggtggtg atatggaaat ccgcctcacc ccaccccgac agatgtctct cgatgaagcg
     7861 atcgaatatc tcgccgacga tgaattgttg gaagtgaccc ctgaaaatta tcgtattcgc
     7921 aagcgtatcc tctccacaga tgagcgcggt aagcagacga aaaaggcgaa agagcaattt
     7981 ggtgaagaat aaaaaatatg ccgacacgaa agtgtcggca tattttttat gatgggagat
     8041 ttagggacag gtgatatttg ccgttgcgct atcggaactg cctgcttcgt tgtaggcctg
     8101 aatttgataa gccacacttt caccagaatt gagatcgatc gtttctgcaa agtattttgt
     8161 gttcggggcc agttcggcca ccttttctcc gtctctgatg attcggtatc ccaattcatt
     8221 ttcggcgttg tcattccagg agagtgtgat atcggccttg cccggaccat agcaaaagaa
     8281 cgtccatccg ttcttgggga aggtggctgc ttcagggccg ccgccttcag gcgtgggcgg
     8341 tgcggtgacc gtgggaactg cggcaaaact tccaacaggc gtggtgaatt cccccgagat
     8401 ccagcaatca ccatccttgg tgttgacgac ccagtaattt ggcggataaa aaccaacgat
     8461 ctttactgaa tcagtaggca ggatctgggt gacgcgttca aagtttgtac ctgggccgct
     8521 gcggcaattg atcacatctc ccacactggc cattggctgg gagaacgtgg gtgtgatcac
     8581 aaccggtgtt gccgcctctg ggtctaactg ctgaccacct gccgcggttg ggctggcaag
     8641 cggtgtggag ttgatgacgg cctgaacggt caacgctgcg gcagtggaaa tttctgagtc
     8701 cgaaggtcct gcggcagatg gcatcgaaca agccgaaatg aaaatccccg ccacaagaca
     8761 caaccccatg aattttttca taatgctcct cattacttta tttttttatg ggcgcgtccg
     8821 ccgatgcgga agaattgcac gcgtccattt ttgtcgcgaa tgaaatcgcc gatgccgttt
     8881 ttcattggct catccagcat cagggtttga tctttttcgt agaaggcaac ccgcatgggt
     8941 ggcatggcag gtccgggcgg gctatcgggc gtgggaaatc cgcccagcgg aatgtgctga
     9001 aagataagtt cgccattctt gagtttcagg tcgaacgctt ctgtgccgat tcgatacgag
     9061 ccgacatatt ccttcaattc gtttgtgctg accttgatcg gtttcggaag tttcggcttg
     9121 atgccaaaat acacctcaag cgcaagtccc aacaggttgt tgttcagcac tcctccatta
     9181 tcgttgttgg tgagcaaagc cagcgcaaag tccttttcgg gaataaatac gaacagggct
     9241 tgctgtccat tggtggcacc gccgtggctg tatcgggtga gtgaacctga acgattgata
     9301 aaccatgtga tccccatctc gccgcgtcca ccagcactga tctgcggcat gcgcatggct
     9361 tccaaagatt tgcgcttgat gatgcggctg ccattcccca tgtgaaagcg cgcataggcc
     9421 agcaaatctt ttaccgtgct gaccacgcca cccacaccgt tgcccgcccg cccaatggcc
     9481 cacgggcgtg agacaaaggt ttttttattt tcgttgtaat gtcccgtcac aaaacgatgc
     9541 gtgatgagca ggtcgcttgg gaaaaagaaa ctcatcttca aacccaacgg gtcgagcagc
     9601 atttcctgtg cggcctgctc gtaaggcttc cccgtcaaca cttcgatcag gcgtgaggca
     9661 atgttgaagc ctgtattgtt gtacgaccag atttctccga gcggcatcac ctgcggaagg
     9721 tgcgcaatgt cctttaccat gcgagccaac gcatcgtcac cgttgccgaa gtcgttgaaa
     9781 taatctccga cccagccgcc tgtatgggta agcaattgac gtacggtgac ctgcttttcc
     9841 accgccttgt cgctcagttt caagtccttg aggtaagtat gcacaggggc atccaggttg
     9901 actttgccct gttccgccag ctgcatgatc agggtgcccg tgaaggtttt gctgatggag
     9961 ccgacttgaa acaacgtatc tgacgtaact ggaagcggat tctcaatact ggtgattcca
    10021 aacccgtcag agaattccct gcctttgtgc caaactccaa tggcaacacc aggtatggat
    10081 agccgcttca tctccgctac aactttttgc gagagcttgc gaaagttttt atccttgatg
    10141 tgggctgtgc tcagattcat cattattctc catcgccttc cagccaggtg gacatgtatt
    10201 caggcttttc catggcagca gcctgtttcg tgagattcag cgggtggaag gtatccacca
    10261 tcactgcgag ttccttcgtt tccactttgc cgatgctggc ttctgtcatg cctggctgcg
    10321 gcccatgcgg aagcccaaac ggatgcaagc tgatatcgct gcggtcaatt cccttgcggc
    10381 tcatgaaatt tccctcggca taatacaaca cctcatccga ttggatgttg gaatgattgt
    10441 atggagcggg gatgccctcg gggtgataat caaagagacg ggggacgaat gaacacacaa
    10501 caaaattatg agcgtcaaag gtttggtgga tgggtggcgg catgtgcagc cgccctgtga
    10561 tgggttcgaa atcttcaatg ttgaagatcc acgggtagag ataaccatcc cagccaacga
    10621 catcgaaagg gtgaaaatca aggatgtgac gtgataatct gtcgcgcact ttcacgcgga
    10681 cttcaaaatc gccgcgttcg gtgtgggtct ccaattcttc aggcaggcgg atatcccgtt
    10741 cacagtaggg ggcatgttcg agcagttgac cgtagttgtt tcggtagcgt tttggcggct
    10801 cgatctggct tggattttcg atggtgaaga atttacattc ctcatttagt tgcatttgcc
    10861 aggtaacccc aaatggaatg acgatgtaat cgcctttgcg aaacggcaga ttcccaaatt
    10921 ggcttttaag cgtgccgctg ccatcatgta cccaccaggt ctcataggct tgtgagttgc
    10981 gataaaaata ccccatgctg tcttttgcgc gtgaaacgcc aaggatgacg tcgttattgc
    11041 cgaggagtgg aatcctcgcc gaaacaggat caggcccgag cggggtttga tccgttctaa
    11101 aggcgcggtg acggatcggg ccaaagtcca catattcagg tttggcgctg ccaaggtcct
    11161 ccgtcatttt cacgcgtggt ggaagaaagt ggtgatagag aatggattga atggaggaaa
    11221 atccctccaa ccccatcact tcttcacgat atagctttcc atcggccttt ttgaattggg
    11281 tgtgacgctt atgcgggatc ttgccaagct tgtgataaaa aggcatgaat atctccttga
    11341 gtggtgacga aaaaatctaa atcaccatgt aggtgttggg acctttgagt tttgcctgtt
    11401 tcaaggcttc ttcggctttt tggatgagtt ctgcagcgga gagctcggcc ccaccattgt
    11461 gggcggtggc accgatgtgc agtgagaatg caatggaacc attggcgacc tcgaatttgt
    11521 ttcgatagac cgacaacaat ctttcgcaaa cggcacgtgc gccatgttcg ttggaactgg
    11581 gcaacaaaac cctgtaggcc ctgccatagg tggatgggat atccaccgaa cgcagatgcg
    11641 aatttagcgc agcggcgaaa accgcgggag cctgagcctg taattctgcg tcgagtgtga
    11701 tgggtgccat ctcaatataa agcagggcca gcggagatgg ataccgcctt gagcgggtga
    11761 cttcataatt gaacaacacc tcaaacacat cgctggtgta ggtatcttca tcacgcgggt
    11821 gcgtattgcg catgtgcttc tccgtttctt ttgtttatga atcctgcggt tgataaacca
    11881 cgacactttc ctgttcgttg gggtcgttgc gcgccaccac agccacaact tcctcggttt
    11941 cgctcaggtt gatgggttga tgcggcaggt tgggcggaat gaaaagaaaa tcgcctgcct
    12001 cgttgatgct ggaatgttcg agcttttcgc cgtacctggt ttcggcccgt cctttgatga
    12061 tgtagatggt ggtctcatag ccattgtgat aatgcgccac ggccctcccg ccaggcggga
    12121 tgataacaag gtgcatgcac aaatgtttgg ttcctgcggt ttgggttgag atgccttcaa
    12181 agttcggtaa ttgctgcttt gagatgaagg tctcttttgg gcggacggtg atgatcttgt
    12241 tctgttccaa tttgtaaatc tcctttcaaa acaacggggt tggttttgtt ccaatggtgt
    12301 tcttcaacgt tccgattttt tcaatttcca attcaaccac atcgcctgcg ttcagccacg
    12361 gcccctggcc tttcgtcaat tccaaaagac aacccgtgcc aaccgtccct gagccgatca
    12421 catcgcctgc ctgtaattca catgtgtctg aggcccttgc aatgatctcg ccgaaagacc
    12481 agaaaatatc ctttaaattt gcgcgggaca tttcaacgcc gttgattttt gccgtcatca
    12541 taagatcata cacgcccggg cgatccactg cccgatcggc aagcgcttcg ctcgtcacga
    12601 tcactggtcc taaagacgag gcaaagtctt tccccttggc tgggccaagc ccgatccgca
    12661 gttcttcgcg ctgcacatcg cgggcggacc aatcgttgaa gatggtgtac ccaaaaatgt
    12721 gtgcaggggc atcttcaggt ttgatgtctc gtccgctttt gccgatgatg acggcgatct
    12781 ccagttcgaa atccattgcc gaagtgtagt gcgggttggg aatcacttcg tcggggccaa
    12841 agatggttcg cgggttggtg taataaaaag cagggaattt gtaccactcc tgcggtacat
    12901 cgcgtccgcg gttttggctg gcggttctca catgttgttc gaaggcgtag gcatcgcgca
    12961 gactggcggg ttgaatgggt gcaaggaatt gaacttcggc aggtgcaaat gattcctgtg
    13021 aagcgaggct ggcagcttta tccgctccga gtgcgatgac ggcatgcata tctgcgaggg
    13081 gcagcgggta gattctgtca ttcgagacga gggcaggcct cggcgcggtg tgaggcgtgc
    13141 ggcaggtggc gaaaatcatg tgggtgaatt ggtcagtaaa caaaagtgac cagtcatcag
    13201 tgtcaatgcc tgacgactgg tcacaggtcg ctacaaattg ccgcggcgtt gttgttccag
    13261 ctcaagtgat tcgaacaggg ctttgaagtt ccctttgccg aatccctgtg cgccgtgtct
    13321 ttggatgatc tcgatgaaca tggtggggcg gtcttgaatg gggcgggtga agatctgcag
    13381 aaggtagcct tcatcgtcct tgtcgaccag aatgccgagc tcctgaatgg ttttgtaatc
    13441 ttccttgatc gagccgacac gctctgggag ggcttcgtag taggtatcgg gtacgcgtaa
    13501 aaattccacg ccgtttttgc ggagttggtc tacggtggtg agaatatcgc ctgtgatgat
    13561 ggcgaggtgc tgtgcgccgg gggtgaggta ataatcgagg tattcttcga tctgactctt
    13621 gcgtttgcct tcagcgggtt cgttgatcgg gaatttgacc cgcccatttc cattttgcat
    13681 gaccttcgac atcagtgcag aatattcagt ggaaatgtct ttgtcgtcga aatgcatgag
    13741 ctggcgaaag cccatgacat ggtgataaaa attgacccac tgattcattt tgccaagctg
    13801 cacgttgcca acgatgtggt caatggcggc cagccccgtg gagtgggctt ccttgtcttt
    13861 gatggggcgg taggtggggg caaatacgcc tttgtagtcg gctcggtcta cgaatgtatg
    13921 cagggtttcg ccgtaggtat aaattgaaga tgtgcggtag atgccgtggt catctttttc
    13981 ttcgcgggga gcccaggccg attttgcgcc gcgggaggtg gtggcctgcc aggctttttc
    14041 cacgtcgtct acttccatgg caatgacttt gaccccgtca ccatgcacca tgtgatgcga
    14101 tgcaagcggg ctggatgggc cgtagggggc cgaaacgacg atgcgcgcct gtcctgattc
    14161 gagcacgtac gaagcgaatt cgcggttgcc cgtttcaaga ccggagtagg ccacgggttt
    14221 gaagccgaag gccttccacc agtaatacat ggcatgcttg gcattaccga cataaaaatg
    14281 aacatgatca atggacttga tctgcagaaa gtctgcttct tctgtcatcg ggcgggtttc
    14341 atcgattgtc tttgttgcca tagattctcc tttgggtttg gttaatggga ttttacgata
    14401 aaggccttgc gtatgcaa
//
LOCUS       DAOQLC010000107        14417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1529_length_14417_cov_14.899039, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000107 DAOQLC010000000
VERSION     DAOQLC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1529_length_14417_cov_14.899039"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="yut"
                     /locus_tag="PL000_16195"
     CDS             complement(<1..925)
                     /gene="yut"
                     /locus_tag="PL000_16195"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNC90474.1"
                     /translation="MNWSLNLDKRIPTPLRILLRGAGQVVFCNNAVTGLIVLAAFYAG
                     GTITGLAATVGLISSTVTAHACRFCKADIEAGLYGFNGVLSGACLSIFLEHTPQLWLY
                     IVLASMLSSLMWAVLTRMLQLFNMPAATSPFVLVSWVFLVTIYAFDSYALGPALPAAS
                     MQLAVMDIGTLPLETWFTALARGIGQVIFTDNTLAGGMLLTGIAIASLRGALMVIGGA
                     FIGVVVPAILGVHASAIEAGLYGFNPALVMIAIGWVFLEPSAKSALLAILAGILAVLC
                     QAVLSSLLTPMGLPVLASPFILTLWVVLFIAS"
     gene            complement(941..1579)
                     /locus_tag="PL000_16200"
     CDS             complement(941..1579)
                     /locus_tag="PL000_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC90475.1"
                     /translation="MLDTGTQARPRHHSRKETNKRKLLQTACRLFCKEGIHATGIARI
                     IEESGVSRRTLYLHFGSKENLLKAVFEAEASIWFRWFDRDLPQMHCSPVERILALFDL
                     MQNWFKSEDFYGCVFINAVAEHDKGNSWIQEIANRHGEKINARLQAMVAQSGIQDPEL
                     VTKKLSLLIDGTIVTAMVTGNGEVAHIGRLTAEDILRSAPCTNNQATKMHSI"
     gene            complement(1642..2829)
                     /locus_tag="PL000_16205"
     CDS             complement(1642..2829)
                     /locus_tag="PL000_16205"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC90476.1"
                     /translation="MKNNDIHDVAVVGAGPAGLSAAYELARAGLEPLVLERTAAVGDV
                     WRNHYDGVRLNSGRYFSALSGSKFPLSAGSWPSREEVVRLLETFPERGGFTVQTNIDI
                     KQVNYDPDRDLWIITSIDGKRFESRAVVMAIGGCRIPVIPKWEGMETFPGEIIHSSKF
                     KNAQDFSGKHVLVIGTGNSAAEIASRLTEHASHVTVSGRTPPHILPKSVYGIPLIGIG
                     VWTRYWPGTIVDRILSFLQRSMIGNLSAHGLPYPTLPLSKQYAINNVVPILYRPFVDD
                     VRAGRIRIVGPIQKITGRTVHVLRTLTTDPGSTHSLTTLEPDVIIAGTGFRTGISKLV
                     QVPGITDENDRSVISGDQEFKDAPRLYFIGQVNPLSGLLREIRLEAGRIAKKIKQQLA
                     AGR"
     gene            complement(2915..3478)
                     /locus_tag="PL000_16210"
     CDS             complement(2915..3478)
                     /locus_tag="PL000_16210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNC90477.1"
                     /translation="MINSTTDIIVNGLDLKRIWAALDSIKADDKGDIANPGYRATVEW
                     DSGFHSTTHTTDGQVVLSDEPKRYGGEEMGATPEDLLLAAVGACLVNTYIAALSAARI
                     HVESLRMNVSGRVNFRTAFGLEAGTPGYENIKIVVDIQTDAPEEKVIALMEKVFPTAP
                     IPDTIMRPVPIDVEIVHHAEATATHAS"
     gene            4061..4792
                     /locus_tag="PL000_16215"
     CDS             4061..4792
                     /locus_tag="PL000_16215"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNC90478.1"
                     /translation="MELDKKEFDPVSKRGNSHKHSKRTIVNAILYVVKGGIQWRLMPN
                     DFPPWQTVYDHFSRWNKRGVWEECLDKLTLFSRQKAGRCAVPSYGIIDAQSVKTQYAS
                     EERGIDGGKKVKGHKRHIVVDILGNLLHVKVHAANVSDTKSACTVLEGVIDKYPSLKA
                     FSGDAGYRGTAVDFVVNGLGLALHISEKIEGKWAVLPKRWVVERTFSWLGNFRRLSKD
                     FEILPATAENMIRIAMMKITLAKCV"
     gene            4817..6886
                     /locus_tag="PL000_16220"
     CDS             4817..6886
                     /locus_tag="PL000_16220"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNC90479.1"
                     /translation="MQEKRSEAQKTLELVDSYLKEHAQDEWLISGLAGVEEQLDGLLS
                     KQKEIVQEEANQEKAVTTLERVTKSLDDCQKQCRTRKQELDDASIRLQQGKDALSQLL
                     GDRLLREYRTEKETLLREMAFLTKIAELEDHRAKLEDGKPCPLCGATEHPFAEGNVPV
                     PDETEQKIKALTKLISKAEDQEAAIKKLEEAEVTARKNLTEGEKQETAAANDKKAAEK
                     SFAEVKEGLEKLRADFTARRQAVSAKLLLLGITEIPEMQVSSLLESLRARLNAWQAQV
                     KKKAEIEKQIADLDSEMKRLDAVIETQSTTLAEKRERLETLKKDHTIGSDERKALYGD
                     KNPDNEELRLNKTVSDAEDAEKKARDRHNELHQRWNTAKTHIESLKKRIDQREPELRK
                     LEIEFSTALAPAGFSDEVQFLEARLTAEQRDELSTKAKKLDDNQTDLKARQKDRETRL
                     ATEIAKMVTNKSLEEMEPQFKKYEDSLKDLRNIIAGLKHKLSENTAAKERIKAKQTAI
                     EAQKKECHRWENLHELIGSADGKKYRNFAQGLTFEMMIGHANRQLQKMTDRYLLVRDD
                     AQPLELNVVDNYQAGEIRSTKNLSGGESFIVSLSLALGLSHMASKNVRVDSLFLDEGF
                     GTLDEEALDTALETLAGLQQDGKLIGVISHVPALKERISTQIQVTPQTGGRSQISGPG
                     CCRNGSV"
     gene            7268..8158
                     /locus_tag="PL000_16225"
     CDS             7268..8158
                     /locus_tag="PL000_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNC90480.1"
                     /translation="MRELETNLLDWQKRYGTEEACAQALTQQRWPEGFRCPRCGHDHG
                     YVISTRHSYECSKCHYQTSLTAGTLFHSTNLSLTKWFWAIYLSASDKGGISAVRLSKQ
                     IQVSWVTACRILRKIRIAMGHRDSIYRLHDLIEIDDALVGGRRSGKRGRGAEGKTPIL
                     VAIENRSKCAGFIAMQQVSAVTHETVAKFVQCHLPPSQLVRSDALASLAEIGKTQHHA
                     ARVSPPDMAGEWLPWVHIAIGNLKAFLLGTYHGVSSKYLQEYLNEFCYRFNRRMWEAE
                     LPLRLLNACLTHTQIKLKIV"
     gene            complement(8423..9388)
                     /locus_tag="PL000_16230"
     CDS             complement(8423..9388)
                     /locus_tag="PL000_16230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC90481.1"
                     /translation="MNPDNLPAIEHSSHRLLDVADFYRLAEVPPEIEWFANLENFCTR
                     RAYKNALQDFMQFTGISHPEEFRQITRAHIIAFRDELRNRDLSGATIRHSLAALSSLF
                     ENLCEQNAVTHNPVKGVKRPSVESYEGKSPALGDHQARALLDAPDSVSLKGKRDRAIF
                     AILLYHALRRDELCKLKVKDVKQERRGVLHMKVSGKGGKTRYLPIHPQVGGWLHDYLE
                     AAGHREDDNGPLFRSLSNNRHKGAQKAITPDAVYKIVQAYSERLGFRIGAHSLRATAA
                     TNALDHQADIAKVQEWLGHANIATTRIYDHRKTRPEDSPTFKVNY"
     gene            9542..9838
                     /locus_tag="PL000_16235"
     CDS             9542..9838
                     /locus_tag="PL000_16235"
                     /inference="COORDINATES: protein motif:HMM:NF015640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HNC90482.1"
                     /translation="MKRTDKAEKISITLPPDMLNIIREKVKDGAYASTSEVIREAMRL
                     WQRQEEEHHARIASIRARLEQSAHSGEPVLLDAAFEKLEQFHQQRINAPGHEKL"
     gene            9825..10172
                     /locus_tag="PL000_16240"
     CDS             9825..10172
                     /locus_tag="PL000_16240"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC90483.1"
                     /translation="MKSYEVYLTPDAIQDLTNIYEYIAAKSGLPEVAWAYIEKLRQKC
                     HDLKTTPLIGQQRDDLRKNLRIVAIDKNAIAAFEVNENKQSVTILNIFYGGRDYEAIM
                     GSETPYKNAELDK"
     gene            10174..11124
                     /locus_tag="PL000_16245"
     CDS             10174..11124
                     /locus_tag="PL000_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC90484.1"
                     /translation="MTDLITTVNRALTAKEFQGLADVPPEVEWFANLGNVETRRAYEN
                     ALKDFMNFTGIQNPEEFRIVTRAHIIAWRDDLLSRTLSSMSIRHRLAAISSLFEYLCE
                     KNTVTHNPVKGVKRPAVESYEGKTPAIGDHQARELLDAPDGSSLKGKRDRAILATLLY
                     HALRRDELCRLKVKDFKQERRGVPHMKISGKGGKTRYLPLHPAASSLIHDYLDAAGHG
                     LEDTGALFRSASNNRDKESQKPITPDGVYKLVQRYSEKLGFKIGAHSLRATAATNALD
                     HQADIAKVQEWLGHANIATTRIYDHRKTRPEDSPTFKVAY"
     gene            11228..11554
                     /locus_tag="PL000_16250"
     CDS             11228..11554
                     /locus_tag="PL000_16250"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC90485.1"
                     /translation="MVWTVETLDQIVDEELEALPSDMRARFVWVSQLIEIHGLQNVRE
                     PYIKHVEDVLWEIRMKGKDGISRALYATVKPERVVVVRVFVKKTQKTPRGEIKLALKR
                     AKEIEQ"
     gene            11551..11835
                     /locus_tag="PL000_16255"
     CDS             11551..11835
                     /locus_tag="PL000_16255"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC90486.1"
                     /translation="MTKVNELHRKWLNDPSYKADFEAITEEFEIASAIIEARNLAGLT
                     QSELAKRLKAKQSLVARLESGADNTTIKTLQRIAQATGTHLKISFEQPGT"
     gene            complement(12192..12824)
                     /locus_tag="PL000_16260"
     CDS             complement(12192..12824)
                     /locus_tag="PL000_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90487.1"
                     /translation="MIKERYLATTLILCSCITMAMADNVIAESTSFSNNNVPSYENGV
                     LTIPRVDTPDQIGNYQDATLKFDPQLNTWILQQVNPAKFNSSNPKIKSVHVFTIDDSF
                     PTQIFLQVVGDFTCGEFGQINTRRKDNLFEVQISVIPTPPGQTCTANITEFVRVIQLD
                     VYRLDAGDYQYSINGDNLGTFSLTEANKFGECGGTRNPEEICEIEVVTAN"
     gene            complement(13138..13617)
                     /locus_tag="PL000_16265"
     CDS             complement(13138..13617)
                     /locus_tag="PL000_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90488.1"
                     /translation="MDETEAERWRKDGRVEYVEQDMILTSGTTQSNPGWGLDRLDETS
                     VTLDNTYVYTNTGAGREIYILDSGLDLSNPTVAAQFGGRASVLWDVNGGTGADCNGHG
                     TQVSSAAAGSTKGIAKGATVIMAKITSGCTGGSSISTSVLVRGQIFLPLLQGNLWHY"
BASE COUNT         4027 a         3258 c         3499 g         3633 t
ORIGIN      
        1 tactcgcaat aaacaggact acccataagg taagaataaa gggagatgcc agaacaggca
       61 ggcccatggg tgtcagtaga ctagataata ctgcctggca cagcacggcc agaataccgg
      121 ccaggatcgc cagcaaggcg cttttggcac tgggctcgag aaaaacccag ccgattgcaa
      181 tcatgaccag ggccggattg aatccataga gtcccgcctc gatagcactt gcatggacgc
      241 cgagtattgc gggtacgaca accccgatga aagcgccgcc aatcaccatc agcgcgccgc
      301 gcagggaggc gatggcaatt ccggtaagaa gcatgccgcc ggcaagcgta ttgtcggtaa
      361 agataacctg gcctatgcct cttgccagtg cagtgaacca tgtttccaga gggagggtac
      421 ctatatccat gacggctaac tgcatgcttg ctgcgggtaa cgctgggcca agtgcgtagc
      481 tgtcgaaggc gtagatcgtt accagaaata cccagctgac caggacaaat ggtgaagttg
      541 cggctggcat gttaaagagc tggagcattc tggtcagaac tgcccacatg aggctcgaca
      601 gcatggatgc caatacaata tagagccaga gctggggcgt atgttcaaga aatattgaga
      661 ggcaggcgcc tgacagcaca ccgttgaagc catataatcc tgcttcgata tcggctttgc
      721 agaatctgca ggcatgagca gtcacagtgc tgctgatcaa gccaacggtt gcagccagcc
      781 ctgtgatcgt tccgcctgcg tagaatgccg ccagtacgat caatcctgtg acggcgttgt
      841 tacagaaaac gacctgtccg gcacccctca gcaaaatgcg tagaggtgtg ggtatcctct
      901 tgtccaaatt taatgaccag ttcaaggtgg tgttcctctt ttaaattgag tgcatctttg
      961 ttgcctgatt gttggtgcaa ggggcgctgc gcaggatgtc ctctgcagtc agtcgtccga
     1021 tatgggcgac ttcgccattg ccggtcacca tcgcggttac gatggtgcca tcgatcagga
     1081 ggctcaattt cttcgtgacc aattcgggat cctgtatacc actttgcgca accattgctt
     1141 gcagcctggc attgattttt tcgccgtgcc ggttggcaat ttcctgtatc caactgttac
     1201 ccttatcgtg ctcggcgacc gcattgatga acacgcagcc gtaaaaatcc tcgcttttga
     1261 accagttctg catcagatca aacaaggcca ggatacgctc aacgggagag caatgcattt
     1321 gcggcaaatc ccgatcgaac cagcgaaacc agatacttgc ttcagcctcg aatactgcct
     1381 tgagcaagtt ttctttcgag ccgaaatgca gatacagcgt gcggcgggat acgcctgatt
     1441 cttcgataat tctggcgatg cccgtggcat gtatgccttc cttgcaaaac aggcggcagg
     1501 ccgtctgcag caattttctt ttattggttt ctttccgtga atgatgtctg ggtcgtgcct
     1561 gcgttccggt gtctaacata gcaatgatcg attgggtact tcgccttcag agcggcagcg
     1621 aagcttgatt gaaaatttcc attatcgtcc cgccgccaat tgttgtttga ttttttttgc
     1681 gattctgcca gcctcgagac ggatttcccg tagcagacca cttaatgggt ttacttgccc
     1741 gatgaagtag aggcgggggg cgtccttgaa ctcctggtcg cccgaaataa ccgatctgtc
     1801 attctcgtcg gtgatgcctg gcacttgaac cagtttggaa attccggtgc gaaaacctgt
     1861 gcccgcgatg atgacatcgg gctccagcgt cgtgagtgaa tgggtgctgc cgggatcggt
     1921 tgtcagcgtg cgcaggacat gtacggttcg cccggtgatt ttctggatcg gaccaacgat
     1981 tctgatccgg ccggcccgga catcgtccac gaaaggacga tagagaatgg gtacgacatt
     2041 gttgatggcg tattgtttgg agagtggcag ggtcggatag ggaagaccat gcgcgctcag
     2101 gttgccgatc atgctgcgct gcaggaagct caatatgcga tccacgatag taccgggcca
     2161 gtagcgggtc catacgccaa taccgatcag tggaatgcca taaacgcttt taggcaggat
     2221 atgcgggggc gttctgcccg atacggtgac atgactggcg tgttcggtca gcctgctggc
     2281 aatttcggcg gcagaattgc cggtgccgat gaccagcaca tgcttgccgg aaaaatcttg
     2341 agcgttcttg aatttcgagg agtgaatgat ctcgccgggg aaagtttcca tgccttccca
     2401 tttgggtatg actggaatgc ggcacccgcc aatggccatg accacagcgc gtgattcaaa
     2461 acgtttgcca tcaatgctgg tgatgatcca caggtcgcgg tcgggatcgt aattgacctg
     2521 tttgatatcg atgtttgttt gtactgtaaa tcctccccgc tcagggaagg tctccagcag
     2581 gcgcaccact tcctcgcgcg aaggccagga gccggcggag aggggaaatt tgctgcctga
     2641 caaggccgag aaatagcggc cggaattcag gcgtacgcca tcatagtgat tgcgccagac
     2701 atcccccacc gcagcggtgc gttccagtac cagcggttcc aggcccgctc tggccagctc
     2761 ataggcggct gacaatcctg ccgggcctgc gccgaccacg gctacatcgt gaatatcatt
     2821 atttttcata gttcaccttg tttggtatgc agttattcat gctcaaaaat gcacgcgact
     2881 ggctgccata aatcccgatg cgcggatgaa gtactcagga agcatgcgtt gcggttgctt
     2941 cagcgtggtg aacaatctcg acatcgatcg gcaccggtcg catgatcgtg tccgggatgg
     3001 gcgcggttgg aaatactttt tccattagcg cgatgacctt ttcttccggg gcatcggtct
     3061 ggatatcgac aacgattttg atattttcgt aaccaggcgt gcctgcctcc agaccaaagg
     3121 ctgtccggaa attgactcgg cccgaaacgt tcatgcgcag ggattccaca tggatgcggg
     3181 cggctgataa agcggcgata taagtattga ccaggcaagc acccacagct gccagcagta
     3241 aatcttccgg tgtggcgccc atttcttccc cgccatagcg tttgggttca tcggaaagca
     3301 ccacttggcc atcggtggtg tgcgtggtgc tatgaaagcc gctgtcccac tcaactgtcg
     3361 cccggtagcc agggttagcg atatcgcctt tgtcatctgc tttgatcgaa tcgagcgctg
     3421 cccatatccg tttcaggtcg agtccattca cgataatgtc ggtggttgaa ttaatcatag
     3481 ctaagtctcc atgatgagcg caggtggtgg aaatgaattc cccttatctg cctgataaaa
     3541 atattttact gattgtcatt ctagcacact tatcgcaaaa agtataccga tcgttctctt
     3601 tttaaatcct cttctaaaac agatggatag cctagaaaat tagaaacctg aggcgcgcga
     3661 ggtgtgttga tgagctatga agaaaggcag catggtcggt tgagtttttc ggtggtgtga
     3721 tgatcaaaga aggcgccagt gcgagactga acgaaacatc aggtcgagaa taaagaaaaa
     3781 taaaagcggt gattgggatt cttgcaaaca ataacctcaa tcgttactct tatttttctt
     3841 gattcgcgac ctgaccccga tattgcgata caaaagctca aatcaatatg aagcagaggc
     3901 agtaaaatga gaaaaattgc ttaattggtg ttcctggaat ttgttgacta cgtcacccac
     3961 tactctttag aagctgtctg gcaaaacgat ttccattaga atcaaggatt ttgataacaa
     4021 gctaagagat gtcatacaaa acggatataa ataatgagga atggaacttg ataaaaaaga
     4081 gtttgatcct gtcagcaaac gtggcaatag tcacaaacat agcaaaagaa ccattgtgaa
     4141 tgccattctg tatgttgtca aaggcggtat ccagtggcgg ctgatgccga acgattttcc
     4201 tccctggcag actgtttacg atcattttag ccggtggaac aagcgtggtg tctgggagga
     4261 atgcctggat aagttgacgc tcttttctcg tcaaaaagct ggacgctgtg ccgtacccag
     4321 ttatggcatc attgatgcac aaagcgtgaa aactcagtac gccagcgaag aacgaggaat
     4381 tgatggtggc aaaaaagtta aaggtcacaa gcggcatatc gtggtagaca ttctcggtaa
     4441 tttactccac gtcaaagttc atgctgccaa tgtgagcgat accaaaagcg cttgtacagt
     4501 gctggaaggt gtcatcgata aatacccttc actcaaagca ttctcagggg atgccggtta
     4561 tcgaggtaca gcagttgatt tcgtagttaa tgggctaggc ctggctttgc acatttcaga
     4621 gaaaatcgaa ggtaaatggg ctgttttacc gaagcggtgg gtggtcgagc gcacgttttc
     4681 ctggcttggt aactttcgcc gtttatccaa ggatttcgaa atcttgccgg ctactgccga
     4741 aaacatgatt cgaatcgcca tgatgaaaat aacacttgca aaatgcgtct aatattttac
     4801 cagacagctt cttaaagtgc aagaaaaacg ttccgaggcc caaaaaactc tggaacttgt
     4861 tgacagttat ctcaaggagc atgcacagga tgaatggctg atcagcggtc tggccggtgt
     4921 tgaagaacaa ttggatggcc tgctttccaa gcaaaaagaa atcgttcaag aagaggccaa
     4981 tcaagaaaaa gccgtgacga ctctggaacg ggtgacaaaa tcacttgatg actgccagaa
     5041 gcaatgccgc actcggaagc aggagctgga cgacgcatca ataaggcttc agcaaggtaa
     5101 agatgcatta agccagttgc tgggggatcg gctgttgcgt gaataccgca ccgagaaaga
     5161 aactctgctt cgtgaaatgg ccttcctgac gaaaatagcg gagcttgaag accaccgggc
     5221 caaattggaa gatggcaaac cctgtccact ctgtggtgcg acagagcacc cattcgcgga
     5281 aggcaatgtt cccgttcccg atgaaaccga acagaagatt aaagcactca ccaaactgat
     5341 cagcaaggct gaggatcagg aagccgctat caagaaactt gaagaagctg aggtcacggc
     5401 ccgtaagaat ctgacggagg gtgaaaagca ggagacagca gcggctaatg acaagaaggc
     5461 tgccgagaaa tcctttgccg aggtgaagga aggtctggaa aaactccgtg ctgattttac
     5521 cgcacgcagg caggccgtct ctgccaaact cctgctgctt ggcatcacgg aaatccccga
     5581 gatgcaagtt tcatcactgc ttgagtcact cagagcacgg ctgaatgcat ggcaggccca
     5641 ggttaagaaa aaagctgaaa tcgagaaaca gattgcagat ctcgacagtg agatgaagcg
     5701 tctagatgcg gtcattgaaa cccaaagtac caccctggcc gaaaagcggg aacgcctaga
     5761 gaccttgaaa aaggatcaca ccatcgggag tgacgagcgg aaagcattgt acggtgataa
     5821 aaatcccgac aatgaggagc tccgcttgaa caagaccgtt tctgatgccg aagatgccga
     5881 aaagaaagcc agagatcggc acaatgaact gcaccaaaga tggaacaccg caaagaccca
     5941 tattgaatct ttgaaaaagc gcatcgacca gagagaacca gaactgagaa agttggaaat
     6001 tgaattttcc acagcgcttg cgcctgcggg cttttcagat gaggtacagt ttttggaggc
     6061 cagactgacc gctgagcaga gggatgagct gtctacgaaa gcaaagaaac tggacgacaa
     6121 ccaaacagat ctcaaggcca gacaaaaaga tagagaaaca cgtttggcca cagaaattgc
     6181 caaaatggtt accaacaaat ctcttgagga gatggaaccg caattcaaaa agtacgaaga
     6241 ttctctgaaa gatctgcgga atatcattgc cggtctcaag cacaagctga gtgaaaatac
     6301 agcagccaaa gagcggataa aagcaaagca aacggctatt gaggcccaga aaaaagagtg
     6361 ccatcgatgg gaaaatctgc acgaattgat cggctccgca gacggaaaga aataccgcaa
     6421 ttttgcccaa gggctgactt tcgaaatgat gattggccat gccaaccggc aactgcagaa
     6481 aatgaccgac cgctacttgc tggtccgtga tgatgcacag ccgctggaac tcaacgtggt
     6541 tgacaattac caagcgggtg agattcggtc cacgaagaac ctttccggtg gtgagagctt
     6601 tatcgtcagc ctatcgctgg cactggggtt gtcccacatg gccagcaaga atgtccgtgt
     6661 ggattcacta tttctggatg aaggcttcgg cacgctggac gaggaagccc tcgacaccgc
     6721 tctggaaacc cttgcgggtc tgcagcagga cggtaagcta atcggtgtta tttcgcacgt
     6781 accagcccta aaggagcgga ttagcaccca gatccaggta acgcctcaaa ctggaggcag
     6841 gagccagata tccggtcctg gatgttgtcg taacggatct gtctagcgga gctgaaattt
     6901 caagaagatg attaagaata aggagacatg aatgaacaaa aaaatcagcg gcgcagagta
     6961 tcctctttcg aaaatattca gctccgactt tgacaacacg tatcacatct gaaactaatt
     7021 gattttatgg tgctgttgag aggagtcgaa cctctgacct actgattacg aatcagcgca
     7081 ttacattact gtagtacccg attaccagga catgtgaatc atttctgaaa aaagataagc
     7141 aattctagcc tatatctttt tatcgcgtgg cactggtgta gcaccatgct ttattttata
     7201 tgcctctgaa ctgttttcag taatttctgc caaatgcttt tagatcaacg atatagggag
     7261 ttgaatcatg agagaactgg aaactaattt gctggattgg cagaaacggt atggaacgga
     7321 agaagcttgt gcgcaggcgc tgacgcagca acgctggccg gaagggttcc gttgcccgcg
     7381 ctgcgggcat gatcatggtt atgtcatcag cacacgccac tcctacgagt gctccaagtg
     7441 tcattaccaa acctcgctta cggcgggaac actgtttcac tccaccaatc tgtcgctgac
     7501 taaatggttc tgggcgattt acctgtctgc ctcggacaag ggtggcattt ctgccgtacg
     7561 tctttctaaa caaatccagg tttcctgggt tacggcttgc cggatattgc gcaagattcg
     7621 cattgctatg ggacacaggg atagcatcta tcggctgcac gatctgattg agattgacga
     7681 tgccttggtg ggtggtcgcc gcagtggaaa gcgaggtcgt ggtgccgaag gtaaaacccc
     7741 aatactggtg gcaattgaga accgaagcaa gtgtgccgga tttatcgcca tgcaacaggt
     7801 ctctgctgtt actcatgaaa cagtagcaaa gtttgttcaa tgtcatctcc cgcccagtca
     7861 gttggtgcgt agcgatgcgc tggcttccct ggcggaaatt ggcaaaaccc agcatcatgc
     7921 cgcacgggtg tcgccgcctg atatggcggg cgaatggctg ccctgggtgc atattgccat
     7981 cggcaacctc aaggcatttc tgttgggcac ttatcacggt gtatcgtcca aatatctgca
     8041 agagtatctc aatgagttct gctaccgttt caaccggcgt atgtgggagg ccgagttacc
     8101 gctacgcctt ctcaatgctt gcctgacgca cactcagatc aaactgaaaa tagtatagag
     8161 gcatattatt ttattggtga gtatggtagg gcttgaacct acgaccaagg aattatgagt
     8221 tctgcttatt cgtatttgac tttataaaag atatttttcc acggttcttt catttagcaa
     8281 ttcgtagaag gctatatgtt tcgtattttg gaagtgcatc tgatggtaag gattggtcaa
     8341 tgggatttat cgaaacccag aatgaaagaa attccttttt tattatcgcc gcgtagccag
     8401 caataagttc cgaatccgcc attcaataat tcaccttgaa cgtcgggcta tcctctggcc
     8461 tagttttgcg gtgatcgtaa atacgagtag tagcaatatt ggcgtgtcct aaccactcct
     8521 gcaccttggc aatatctgcc tgatgatcga gcgcattggt cgcagccgtg gcacggagcg
     8581 aatgtgcccc gatcctgaat cccagtcttt ccgagtaggc ctgcacgatc ttatagacgg
     8641 catctggggt aatggctttc tgtgcaccct tatgtcggtt attgctcagc gatcgaaata
     8701 aagggccatt atcatcttct ctatggcctg ccgcctctag ataatcatgc aaccatccgc
     8761 ctacctgagg atggattggt aagtagcggg tcttgccacc cttgccggat actttcatgt
     8821 gcagtacccc acgtcgttcc tgtttgacgt ccttcacctt taatttgcac agttcatcgc
     8881 ggcgcagcgc atggtaaagc aatatggcga agatggcgcg atcccgtttt cctttaagcg
     8941 agacactatc aggcgcatcg agtagcgcgc gcgcctgatg atcgcccagt gccggagatt
     9001 tgccctcata gctttccacg ctagggcgct tcaccccctt gaccggattg tgtgtgaccg
     9061 cattctgttc gcacaagttt tcaaacaatg aggaaagggc tgccaggcta tgacggatgg
     9121 tggcacccga caaatcacga tttctgagct catcccggaa cgcaatgata tgtgcgcgag
     9181 taatttgccg aaattcttct gggtgagaga taccggtaaa ttgcataaaa tcctgcagcg
     9241 catttttata agcgcgccgc gtgcaaaaat tttctagatt ggcaaaccac tcgatctcgg
     9301 ggggaacttc tgctagccga taaaaatcgg ccacgtctaa gagtcgatgt gaggagtgct
     9361 cgatggcagg taaattatct gggttcatgg aaagtatctt actattgata aaatatgtta
     9421 tcaatagtaa agtagatatg acatcaagga aggttgaaat aattttgctt tattaaattt
     9481 cattgagtag gatattatcc taccattgaa aggtgttaga aacatacata ggaaacaaaa
     9541 aatgaaacgc accgataaag cagaaaaaat cagcattaca cttccaccgg atatgctgaa
     9601 tattattaga gaaaaagtga aggatggcgc ttacgcttcc acaagtgagg ttattcgtga
     9661 agcgatgcgt ttatggcaaa ggcaggaaga agaacatcat gcgagaattg cttccattcg
     9721 agcgcgtctg gaacaatccg cacacagtgg tgaacctgtt ttgctggatg ctgcgtttga
     9781 aaagctagag caatttcatc aacaacggat aaacgcacca ggccatgaaa agctatgaag
     9841 tgtacttgac acccgatgcg atacaagacc tgactaatat ttatgaatat atagctgcaa
     9901 agagcggact tcctgaagtc gcgtgggcat atatagagaa attacgccaa aagtgtcatg
     9961 accttaaaac aacacctttg attggacaac agcgcgatga tttgcgcaag aatctcagaa
    10021 tcgttgccat tgataaaaac gcgattgcag cttttgaggt taatgagaat aaacaaagcg
    10081 tcaccattct taatatcttt tatggtggta gagattatga agcaattatg ggtagtgaaa
    10141 cgccgtataa aaatgctgag ctagacaaat aacatgacag atcttattac tacagtcaat
    10201 cgcgcactta ctgcaaaaga atttcagggc cttgcagatg ttccacctga agttgaatgg
    10261 tttgccaact taggtaacgt cgagactcgg cgtgcctatg aaaatgcctt aaaggatttt
    10321 atgaatttca cgggcattca gaatccggaa gaattcagaa ttgtaactcg tgctcatatt
    10381 attgcctggc gcgatgattt gttgagtcga acattaagta gcatgagcat tcggcaccgt
    10441 ttggcggcaa tatcttcttt atttgagtac ctgtgtgaga aaaatacagt tacgcataat
    10501 ccagtcaagg gtgtaaagcg accagcggta gaaagttatg aaggaaaaac tccggcaatc
    10561 ggcgatcatc aggcacgtga attgctcgat gcgccggatg gtagctcact taagggcaag
    10621 cgcgatcgtg cgatactggc caccctactc tatcacgcac tgcggcgtga tgaattgtgc
    10681 cgattgaaag tcaaggattt taagcaggag cggcgtggtg tgccacatat gaaaatatcc
    10741 ggcaaagggg gcaaaacacg atatttacca ttgcatcctg ccgccagtag cttaatccat
    10801 gattatctcg atgcagctgg gcatggcctg gaagatacgg gggcattatt ccgttcagcc
    10861 agtaataatc gagataagga atcacaaaaa ccaattacgc cagacggtgt ttataagttg
    10921 gtacagagat actctgaaaa actggggttc aagatcggcg cacattcgtt acgcgcaacg
    10981 gcagccacca atgcactcga tcatcaagca gatattgcga aagtgcaaga atggttgggc
    11041 catgccaata ttgctaccac aagaatttat gatcatcgca aaacccggcc agaggatagt
    11101 ccaacgttta aggttgctta ttgattgctc tattacccat tgatgaagga atcctcgcga
    11161 ctgaccaata tgtcagtcgc gattaaagaa tcttttgcag tctataacat atatgttata
    11221 ttaaatcatg gtatggactg ttgaaacatt agatcaaatc gttgatgaag agcttgaagc
    11281 cttgccttct gatatgaggg cgagatttgt ttgggtcagt caactgattg agatacatgg
    11341 tttacaaaat gtgcgcgagc cttacataaa acatgttgaa gatgttttat gggagatccg
    11401 tatgaaaggc aaagatggaa tttctcgtgc actgtatgca acagtcaagc ccgagcgagt
    11461 agtcgtggtg cgtgtttttg taaagaaaac gcaaaaaacg cctcgcggag aaatcaagct
    11521 agccctaaaa agagcaaagg agattgagca atgactaaag taaatgaact ccacaggaag
    11581 tggcttaatg atccaagtta caaagctgat tttgaggcaa tcactgagga atttgaaatt
    11641 gcttctgcaa ttattgaggc caggaatctg gcaggattga cacaatctga gttagctaag
    11701 cggttgaaag ctaaacaatc actggttgca cgccttgaga gtggagcaga caacacaacg
    11761 ataaaaacgc tacaacgtat tgcacaagcc actggcacac acctcaagat ctcttttgaa
    11821 caacctggca cctaatgggg ctcttttacc caaagtacaa tttgtcgcgt tgcgcactgc
    11881 gggtagttac ctcatagctg tgaaaactgc catatacgac aagactttgt aagactaatg
    11941 acgcattacg tacctttgtt ggaatttacc cataaatgtg gaatcgatgc aaatgctcat
    12001 cgtcccagcc aaaaacgatt tgaatcacgt ggtgaaagtc agctaatgac gttcgatctg
    12061 gaatccggag gcgacgccag atcattggac tcacccgcga agagcgatct tgatggagtc
    12121 atgtggaagg aaaaaaccac cgactaactg ccggtggcgt gtttacaaac cgtatatcta
    12181 cagtgatgtc atcaatttgc tgttactact tcgatctcac aaatttcctc tggatttctt
    12241 gtaccaccgc attcaccaaa tttattagcc tcagttagac tgaacgtacc taaattatca
    12301 ccattgatac tatattgata atccccagca tctagacgat atacatctag ctggataact
    12361 ctaacaaatt ccgtaatgtt tgcagtacat gtctgtcctg gcggtgtcgg aattacagat
    12421 atttgaacct cgaacagatt atctttcctg cgcgtattaa tttgtccaaa ctcaccgcat
    12481 gtgaaatctc ccacaacttg taagaagatt tgcgttggaa aagaatcatc tatggtgaaa
    12541 acgtggactg atttaatttt tgggttggaa gaattgaatt tcgctggatt aacttgttgt
    12601 agaatccagg tgtttaactg tggatcaaac ttaagcgttg catcctgata attcccaatc
    12661 tgatccggcg tgtcgacacg gggaatcgtc aaaacaccgt tttcataact aggtacattg
    12721 ttattcgaaa aacttgtgct ttccgctatt acattatcgg ccattgccat agtaatacag
    12781 ctgcacaaaa tcaatgttgt agctaaatat ctttccttaa tcatacagta gctcctataa
    12841 agcagacagg acgctatgaa acctaaacgc ctatccactt ttgaatattt attttgtaaa
    12901 cagttgctta ccattgctgc actataaatc gagacgttgc gccggttaac ggagtgccgt
    12961 tagtattggt aacagtgcca attacacctg tgttcctgag tgcatcataa agcgttgttg
    13021 catttgtcgc agtattacat aaagtacccg cagcttggca cgccactgca aaaataccag
    13081 caatataagg tgcagagaat gatgttccat tatttgtgac ggatgttcca ttgaaattca
    13141 atagtgccac agattcccct ggagcaaagg cagaaatatt tgaccccgta cgagaaccga
    13201 agtagatata gatgagcctc cagtgcaacc tgatgtaatt ttggccatta tcaccgtagc
    13261 ccctttagca atgcctttgg tgcttccagc agcagcacta ctaacttggg tgccatgacc
    13321 attacaatct gcaccagttc caccattcac gtcccaaagt acagatgccc gtccaccaaa
    13381 ttgagcggcg actgttggat tcgataaatc caagccagaa tcgagaatat agatttcgcg
    13441 accagctcct gtatttgtat aaacatacgt attatcaaga gtaactgatg tctcatctaa
    13501 cctatccagt ccccaccctg gattgctctg ggtggtacca ctggttaaga tcatatcttg
    13561 ctcaacgtat tctacacgtc catcctttct ccatctttct gcttcagttt catccattaa
    13621 tacgtggatt gcgttaatag tgtcaaatat agatactatt tctcctcgaa gccctaattt
    13681 gttaactaac tcttctttat tctgaccagt actatgttct ccaaaaggaa ccgttccact
    13741 atcccttttt gactcatcag gtggttgaat taaagggtct tctttattat taagaaaagc
    13801 ggatggtttt ttaaaaccaa taatataagg gtattgttaa ttaagtatag aaaacaggtt
    13861 ttatttatat tacattcaat tagttgaaag ttaataacta tatagattta acaatgccaa
    13921 tataagaacc ttctactaat ttttccccat tactaattgc ttgttcaatt ttataattta
    13981 cgtgatcccg attcgcctgc gcctggaaat taatgaaaca aagaaacaag attggtacta
    14041 tatattttat aagacgtctg caagaaatat tcattacaat taattcctta aatgtgaaag
    14101 gattgtttga tactatcagt aatactatcg ttacattagt gataaggatg aaagaggaag
    14161 ataagataac acttactacc atcatatcag tttatttctt taaagaagaa aagtaaacca
    14221 actacttagt agacgaatca tataaaggta ggtgacgctc tgtttatgca gaagaaccta
    14281 tacttacagt taaatttggc accgtatgac agctatgatg taattaggca aaccacacct
    14341 ctcacttacc ccaagtaaaa aacggctcaa ccacatgttg aatctgcaaa cccgcaatca
    14401 gcccaaaata ccgcgca
//
LOCUS       DAOQLC010000108        14079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1596_length_14079_cov_27.945807, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000108 DAOQLC010000000
VERSION     DAOQLC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1596_length_14079_cov_27.945807"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..628)
                     /locus_tag="PL000_16270"
     CDS             complement(20..628)
                     /locus_tag="PL000_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90489.1"
                     /translation="MIRVAVVSPNSALRVGLRELLGRQPDIQVIGEAVDLESVHEMEA
                     EVFVLASVSSGRLLENSITSPILFLTDDIESVRMVLGSKVRAWGVLSLEATEDELAAA
                     VRAVGEGLWVGAPGLVGEMIRFSGRRESLGEESLTEQLTAREKEVIQLMAQGLANKQI
                     ALSLGISEHTVKFHLSSLYSKLGISSRTEAIKRGIELGLISL"
     gene            complement(629..1528)
                     /locus_tag="PL000_16275"
     CDS             complement(629..1528)
                     /locus_tag="PL000_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC90490.1"
                     /translation="MSKNIFVELSNAIADAAEQAGKFTVLVDARRRFPASGIVFAKDL
                     ILTADHVVEREEDINVILADGTEVTARLVGRDSGTDLAVLKLDSASATPAEVSKTPAR
                     VGQFVLAVGRPSSNGIESSFGSVNSLGGPVRTGRGGMLEKYIKTDVVSYPGFSGGPLV
                     NGDGTVLGINTSGFGNSGAITIPADVAWKIAEALAKHGKIKRGYLGIRSQVVELSADG
                     KKALKREQESGLLLVGLEADSPAGKGGLIVGDILVGIAGEPVAHQDDLFARLSGDVVG
                     KSTPVDVLRGGKLETLKVEIGER"
     gene            complement(1634..2566)
                     /locus_tag="PL000_16280"
     CDS             complement(1634..2566)
                     /locus_tag="PL000_16280"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNC90491.1"
                     /translation="MRFAIFGTGGVGGYFGGKLAQVGEDVTFIARGKNLESLKNSGLR
                     VDSILGDFVLEKVQATDLPHSLKNIDVILLATKTWQLDSAIAEMKPMLGEQTAILPLL
                     NGMEHMDTLRAEFGGHVLGGLCRISSFLIAPAHVSHVAVNPHIEFNEWKQETSPRVGE
                     VLSAFSKIKEITVDTPPDIELAMWEKYLLICAFSGVGAITRQPVGVFRSDPESRAMFI
                     RSLHEVVAVARARGVNLTEKSAESVLARIDQTQPDMMASMQKDVMEGRPSELEAQIGA
                     LVRMANAANIPVPTNEFIYSTLLPLEQKARSKAT"
     assembly_gap    2664..2763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3002..3391)
                     /locus_tag="PL000_16285"
     CDS             complement(3002..3391)
                     /locus_tag="PL000_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90492.1"
                     /translation="MANAEERMKILKMIEEGKISADEGSKLLAALSGPRKGVPTPPRP
                     PSVGNSTPRWLRIRVTDTRTGRSKASVQIPLALVDAGMKIGAHFAPEVEGVDMSNVME
                     ALRMGVTGKIIDVTDEEDGEHVEIYVE"
     gene            complement(3401..4039)
                     /locus_tag="PL000_16290"
     CDS             complement(3401..4039)
                     /locus_tag="PL000_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90493.1"
                     /translation="MSSEERRKILQMVADGKISAEEAAGLMRALDEDAGEEDSAGEQI
                     EVLQAESGFGPEQTEAPEFDEVRRRAFRFAMIPLWIGVIVTVLSAWGMYSVQMGAGLN
                     FWFFCLTLPLLFGVLLIALGAGGRASRWIYVNVDRSHQNEWPRKITLALPIPLGFVSW
                     FLKNFGSRIDGLKKTTVDDVLMAISMTKSVTEPLIVHVNDDNSGDRVQVFIG"
     gene            complement(4039..5130)
                     /locus_tag="PL000_16295"
     CDS             complement(4039..5130)
                     /locus_tag="PL000_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90494.1"
                     /translation="MSKTISAGRSPRVVIENIEGDLSLVGWEGDDILLKGDEDEMHIR
                     QDGNQVIISCDDDLSLRVPKSASVSIKKIGGDAAMRGVMGDIELNEIEGDLSIRDVNS
                     VSIGTIHSDFSLRGAKGSIAIKKVLSDVSIRDVDGNVSLESVADDLALRDVRGNVSAN
                     VAEDVVLYLDPKPGNAYAVTAGEDILLVMPPRANATLTLSADEIDIEWKGVDNDDDAT
                     SRVVTLGDGSALVTLSAGGDIRVTNQSNAGDSAEDFGNFAGIGMDWSGFGERISRQVE
                     QATKRATRQADEAIRRAAANVERRAKVNVNAKVGRWNWDLSPKGVPVTPPSQASEEER
                     MAILKMLQEKKISAEDAEKLLSALEGGES"
     gene            complement(5134..5517)
                     /locus_tag="PL000_16300"
     CDS             complement(5134..5517)
                     /locus_tag="PL000_16300"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNC90495.1"
                     /translation="MRPAPTRCPICQSELTVARLHCSSCDTTIEGNFASGQFSNLTPE
                     QLEFVFTFVRCEGKINRMEQEIGLSYPTIRNRLHEIIRALGYEPGRDEPVEITQEKRN
                     SILDDLHAGKITADEATRLLRGEEE"
     gene            complement(5534..6790)
                     /locus_tag="PL000_16305"
     CDS             complement(5534..6790)
                     /locus_tag="PL000_16305"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90496.1"
                     /translation="MLNNTIEKATKLYREYPRTFWILIVITFIDRIGGALLFPFFALY
                     LTSKFGVGMADVGVLFAAFSVSSFVGSAVGGALTDRFGRKGIIIFGLIASSFSTVAMG
                     LVTSFEYFFVLAFFIGILTDVAGPAHQAMIADILPEDKRADGYGILRVAFNLSVVIGP
                     AIGGLLAARSYLMLFLSDAAISLLTVILIAIFLPETKPEAHPDAPKESMTRTFAGYGQ
                     VFRNTAFMLFLGAVLLQVFTYMNMNTSLGVFLRNEYGINESGYGLLLSINAAMVVLMQ
                     FPITRRITNFPPMLMMAAGTFLYVIGFSMYGFVTTYAMFVAAMVIITFGEMVVSPVAQ
                     ALVASFAPEEMRGRYMAVSGFSWGIPFAVGPYLAGLIIDGPSPHMLWYAAGIVGTLST
                     VGFLVLYRVKSKQTQNLELMPEVTAS"
     gene            complement(6780..7019)
                     /locus_tag="PL000_16310"
     CDS             complement(6780..7019)
                     /locus_tag="PL000_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90497.1"
                     /translation="MDILSERMSELKRKSIQEDLDAIHLEEEAVKGKTLLDKNLAWIG
                     NWMVSRGEKLRKRYNQSLHEANSTQLVKKAAYHAK"
     gene            7210..8196
                     /gene="corA"
                     /locus_tag="PL000_16315"
     CDS             7210..8196
                     /gene="corA"
                     /locus_tag="PL000_16315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNC90498.1"
                     /translation="MIRSLFFSPGKPVRTNIPTEEFPRLLRDRRGLLWVDFEAEPEES
                     ALPILNGFGFHQLAIDDALQQTHAPKIDDWGDYLYIVLNYMHLTKATEPWDTEIDELD
                     IFLGRNYVITHHDNPITSINETWNTSQRDPRYSQDGADHLLYKIIDAIVMNYMPIIEK
                     IDEEIDWVEDQVFDRPSSQTLARLFTLKRVLLAMRRILLPQREVLNKMARDDFQVIDR
                     KDRIFFRDIYDHLVRLHDVNESLRDLVGGALDTYLSVINNRMNEVMKTLTIITTLFMP
                     LTFVTGFFGMNFFAANPPYESWTLPAVFYGTLSLMLLSPIIMFIWMRRHTWV"
     gene            8309..8986
                     /gene="lexA"
                     /locus_tag="PL000_16320"
     CDS             8309..8986
                     /gene="lexA"
                     /locus_tag="PL000_16320"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNC90499.1"
                     /translation="MMMVRKSKGLGERHQKILDFIASYQRDHKHPPSIREIGEHCDIS
                     STSVVNYYLDQLEKSGNIERDRKISRGVRLTGSTPLGDMFRVPVLGVIQAGEPIPVPS
                     NADFNAFTPEDSVEIAMSLMPTKEKGKELFALKVQGESMIDAMVNDGDIVILKPTQDA
                     RNGDMVAVWLSDRNETTLKFFFKEKDKYRLQPANPTMSPIYVNKNEALEIKGKVVMVI
                     RKVERYM"
     gene            complement(9046..9429)
                     /gene="rplL"
                     /locus_tag="PL000_16325"
     CDS             complement(9046..9429)
                     /gene="rplL"
                     /locus_tag="PL000_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNC90500.1"
                     /translation="MSDKLAKLVDELSTLSVLEAADLVKMLEEKWGVSAAAPVAVAAV
                     AGGAAASAEPVEEKTEFDVVIKDAGAKKIDVIKVIRQLTSLGLGEAKTLAETAGGKVL
                     SAVGKDAAMDAKKKLEEAGAVITVE"
     gene            complement(9502..10032)
                     /gene="rplJ"
                     /locus_tag="PL000_16330"
     CDS             complement(9502..10032)
                     /gene="rplJ"
                     /locus_tag="PL000_16330"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNC90501.1"
                     /translation="MAISKQRKEEVQAQYADWVKRSEAVILVEYSGANMKSIDAIRSK
                     IRETGGEFHIVKNTLARRVFADNGMDVPADYLVKSTAVSFAFKDPASTAKALTDAIKG
                     NEFVKVKGGLMGGKSLSPAQVKALSDMPPLPVMRATLLGVLQAPAGKLVRTLAEPARG
                     LAAVIKARSESAQASA"
     gene            complement(10253..10966)
                     /gene="rplA"
                     /locus_tag="PL000_16335"
     CDS             complement(10253..10966)
                     /gene="rplA"
                     /locus_tag="PL000_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNC90502.1"
                     /translation="MAKHGKKYTAAAAKVDIDKVYTAREAVSLAKETSITKFDATIEV
                     HMRTTLDSRQADQQLRDVVVLPHGLGKTVRVLVFAQGEGAAAARAAGADLVADDDETM
                     KKIEGGALDFDVAIATPDAMGKVGRLGRVLGPRGLMPNPKAGTVVGAADMERAIKEAK
                     AGRVEYRLDKTNNVHVSIGKASFGADKLFENYVALMDAVNKSRPSGAKGNFVKRITIS
                     STMGPGIKTDPNEHIDGAE"
     gene            complement(11017..11442)
                     /gene="rplK"
                     /locus_tag="PL000_16340"
     CDS             complement(11017..11442)
                     /gene="rplK"
                     /locus_tag="PL000_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNC90503.1"
                     /translation="MAKKLKAIVRLQLEAGKANPAPPVGPALASHGINIMAFCKEYNA
                     RTSNRPGEVLPAEITIYTDGSFTFVLRTPPAAVLLRKAAKVEKGSGVPNKDKVGKVTR
                     AQVKEIAELKMKDLNAINIEGAMKQVEGTARSMGITVTD"
     gene            complement(11545..12240)
                     /gene="nusG"
                     /locus_tag="PL000_16345"
     CDS             complement(11545..12240)
                     /gene="nusG"
                     /locus_tag="PL000_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNC90504.1"
                     /translation="MMDLPESQEQFEQEPMGEQTEEQNTPAVVSVSSSDTKPVPASES
                     QIPTDLPPVETIVEGPAWYVIHCYSGYENKVRHNLEQRIETMGMKDKIFDVVIPTQEE
                     IEVKDGKRRTVERHIFPGYVLVNLTLSEESWYVVRNTPGVTGFVGMGNSPTPLRPEEV
                     AQIVKRMEAEAPTVKVSFKVGERVRIVDGPFNDFRGVVSEIDMERTKVRVMVSFFGRE
                     TPVELDFLQVEKA"
     gene            complement(12257..12475)
                     /gene="secE"
                     /locus_tag="PL000_16350"
     CDS             complement(12257..12475)
                     /gene="secE"
                     /locus_tag="PL000_16350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNC90505.1"
                     /translation="MAEKEKKVKKSDNAVQRYFRETSGELRKVSWPTWPEARRLTELV
                     LAVMVIVGFFLAIIDYVSDLLINLILGI"
     gene            complement(12539..12611)
                     /locus_tag="PL000_16355"
     tRNA            complement(12539..12611)
                     /locus_tag="PL000_16355"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12576..12578),aa:Trp,seq:cca)
     gene            complement(12687..12836)
                     /gene="rpmG"
                     /locus_tag="PL000_16360"
     CDS             complement(12687..12836)
                     /gene="rpmG"
                     /locus_tag="PL000_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNC90506.1"
                     /translation="MRPVITLQCSDCKERNYTTEKNRRNDPNRLEFNKYCSRCKKHTQ
                     HRETK"
     gene            complement(12880..14079)
                     /gene="tuf"
                     /locus_tag="PL000_16365"
     CDS             complement(12880..14079)
                     /gene="tuf"
                     /locus_tag="PL000_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNC90507.1"
                     /translation="MAKEKFDRSKPHLNVGTMGHIDHGKTTLTAAITKVAGLAGQAEF
                     KAYDQIDNAPEEKARGITINIAHVEYQTDKRHYAHVDMPGHRDYIKNMITGAAQVDGA
                     ILVVAAPDGPMPQTHEHVLLARQVEVPSIVVFLNKVDMMDDPELLELVELELRELLNK
                     QGFPGDTTPIVRGSAKQALESAATDPNAPEYAPIRELLRVIDEYIPEPKRDTDKPFMM
                     SVEDVFSIKGRGTVVTGRIDRGVVKVGEPIEIVGLRETKSTVCTGVEMFHKQLDEGMA
                     GDNVGLLLRGIEREDVERGQVLAKPKSITPHKKFLAEVYVLKKEEGGRHKAFFSGYRP
                     QFYVRTMDVTGDIKLPEGVEMVMPGDNVNLTVELIVPVALEQGSKFAIREGGLTVGAG
                     VVTKIIE"
BASE COUNT         3528 a         3867 c         3193 g         3391 t
ORIGIN      
        1 gctggcggga tgtttgttct taaagcgaga tcaagcccaa ctcgattccc cgtttgatgg
       61 cttcggtacg gctggaaatt cccagtttcg aatacagcga tgacaaatga aatttcacag
      121 tatgttcact gatccccaac gaaagtgcga tctgtttgtt cgccaacccc tgcgccatta
      181 attgaatgac ttccttctcg cgggcagtca attgctctgt gagagactct tctcccaaag
      241 attccctgcg ccccgaaaag cggatcattt ctccgaccag gcctggtgcc ccaacccaaa
      301 gcccttcccc cactgcacgc accgccgccg ccaattcatc ttccgtggcc tcgagtgaaa
      361 gcacacccca ggcacgcacc tttgaaccca acaccatacg gacagactca atgtcgtctg
      421 tcaaaaacag gatcggagag gtgatgctgt tctccaacaa acgaccagaa gagaccgagg
      481 caagcacaaa tacctcggcc tccatttcat ggacgctttc aagatcaacc gcttcaccga
      541 tcacctgaat atcgggctgc ctgccaagca gttcacgcag accgacccgc aaagcagaat
      601 tcggcgaaac gaccgcgaca cggatcaatt atctttctcc aatctccact ttgagcgttt
      661 ccaattttcc tccacgcaac acatccactg gagtggattt accaaccaca tcaccactca
      721 aacgggcgaa caggtcatct tgatgcgcca caggttcgcc tgcaatcccc acaaggatat
      781 cgccgacgat caaaccgcct ttgcctgcag ggctatcggc ttcaagacca accaataaca
      841 aaccgctttc ctgttcacgc ttcagggctt tctttccatc tgcagagagt tcaacaacct
      901 gactgcggat gcccaggtac cctcgtttga tcttgccatg cttcgccagc gcttcggcga
      961 ttttccatgc tacatccgca gggatggtaa ttgcgccaga gtttccaaag cctgaggtgt
     1021 tgataccgag tacggtacca tccccattga ccagggggcc gcctgaaaag ccagggtagg
     1081 aaaccacatc ggtcttgatg tatttttcca gcatacctcc acgtccagtg cggacgggac
     1141 cgccaagcga attaaccgag ccaaagctgg attcgattcc gttggaagaa ggtcggccaa
     1201 ctgccaaaac aaattgcccc acgcgtgcgg gtgtcttgga aacttcagcg ggagtggctg
     1261 aggctgagtc taatttcaaa actgccagat cagttccaga gtcccgacct acgaggcgcg
     1321 cagttacttc cgtgccatca gcaaggatca cgttaatatc ctcttcgcgt tccaccacat
     1381 ggtctgccgt caaaatcagg tctttggcaa atacaatgcc gctggcagga aaacgtcggc
     1441 gcgcatccac taacacagta aatttgccag cctgttcggc ggcatcggcg attgcgtttg
     1501 aaagttcgac aaaaatattt ttactcatgg ttatctcctt gcgcatcagt atagcgaatg
     1561 catctctgga gtgtatcgcc taaagggatg gatttgtact ggaaaaatgg gtaggttggt
     1621 aaatggtaaa aaatcaggtt gctttgctgc gggctttttg ttcaagcggc aggagggttg
     1681 aataaatgaa ttcgttggtt ggcacaggga tatttgccgc attcgccatg cggaccaggg
     1741 caccgatctg cgcctccaat tcggagggac ggccctccat cacatctttt tgcatcgaag
     1801 ccatcatatc tggctgggtt tgatcaatgc gagccaaaac actttcagca gatttttcgg
     1861 ttaggttaac accgcgcgca cgggccacgg caaccacttc gtgcaacgac cgaatgaaca
     1921 tggcgcgtga ttcaggatcg gagcgaaaca ctcccacagg ctgacgggtt atcgccccca
     1981 cgccgctgaa ggcacagatc aaaagatatt tttcccacat ggccaattcg atatcgggcg
     2041 gggtatctac cgtgatctct ttgatctttg aaaacgccga caaaacctca cccacccgcg
     2101 ggctggtttc ctgtttccat tcgttaaatt caatgtgtgg attcaccgca acatgactca
     2161 catgggctgg tgcgatcaaa aatgaactaa tcctgcacaa gccgccaaga acatgcccac
     2221 caaattccgc acggagtgta tccatgtgtt ccatgccatt caacagaggc aggatcgctg
     2281 tctgctctcc taacatgggt ttcatttcag caatggcaga atccaattgc catgttttgg
     2341 tggcaagcaa aatcacatca atatttttga gcgaatgcgg taaatcggtg gcttgcacct
     2401 tttccagaac aaaatccccc agaatcgaat ccacacgcaa acctgaattt ttcaaggatt
     2461 caaggttctt tccacgtgca atgaaggtga catcctcacc gacctgggcg agttttccac
     2521 caaagtatcc gccaacacca cctgtaccaa agatcgcaaa tctcattcaa atctcccgtg
     2581 atctacatta taaaattcaa aaagcaaatt cggacagggg cttaatataa cccaagttgc
     2641 cgcctgcaca aaaacaggga aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnttcggac aggggcttaa tataacccaa gttgccgcct gcacaaaaac agggaaaaag
     2821 atgtactcgc gtaatttaca tcagaatttg aaggcagcct cttgcgttaa tataaacaac
     2881 cctccgaaga aggttgttta tagcggatag cagccgcttc cccccaggct gttacaaaca
     2941 gttttgacct gcaaacttta ccccgccatg caacgcaaag cagaataaaa atataaagat
     3001 gttattccac gtagatctca acgtgttcgc cgtcttcctc atcggtcacg tcaatgatct
     3061 tgccggttac gcccatgcgc agggcttcca tcacattgga catatccacg ccttccactt
     3121 cgggagcgaa atgcgcgccg atcttcatac cggcatccac caatgcaagc ggaatctgca
     3181 cagaagcctt ggaacggccc gtgcgagtat cagtcacacg gatgcgaagc cagcgcggag
     3241 tcgaattccc cacgctgggc gggcgcggcg gtgtgggcac accttttcgc ggcccgctca
     3301 aagctgcaag aagcttcgaa ccttcgtctg cgctgatctt cccttcttca atcatcttca
     3361 atatcttcat tcgttcttct gcgtttgcca tgttaacctc tcatcctatg aacacttgca
     3421 cacgatcacc gctattgtcg tcgttcacat gaacgatcaa cggctcggtg accgattttg
     3481 tcattgaaat cgccatcagc acatcatcca ctgtggtttt cttcaaccca tcgattcgag
     3541 aaccgaaatt cttcaagaac caactcacaa aaccaagcgg aatgggcaaa gccagggtaa
     3601 tcttcctcgg ccactcattt tgatgcgaac gatccacgtt gacatagatc cagcgcgaag
     3661 ccctgccacc cgcacccaac gcgattaaca gaaccccgaa caaaagcgga agtgtcaggc
     3721 aaaagaacca aaaattcaaa ccagcaccca tctgcacaga atacattccc catgcgctca
     3781 gcacagtgac aatgaccccg atccaaagcg ggatcatcgc aaaacggaat gcccgcctgc
     3841 ggacttcgtc aaactcaggg gcctcggtct gttcgggacc gaaacctgat tcagcttgaa
     3901 gaacttcgat ctgctccccg gcagaatcct cttcacctgc gtcctcgtcc agtgcgcgca
     3961 ttaatccagc ggcttcttca gcgctgatct ttccatcagc caccatctgc aaaatcttcc
     4021 tgcgctcttc cgatgacatt aggactcccc tccttccaat gcagaaagca atttctcagc
     4081 atcttctgcc gaaatcttct tttcctggag catcttcaaa attgccatgc gttcttcttc
     4141 cgaagcttgt gaaggcggtg tcactggcac accctttgga gatagatccc aattccaacg
     4201 ccccaccttc gcatttacat tcaccttggc acgtcgctct acatttgcag ccgcacggcg
     4261 aatggcttca tcagcctgac gggtggcccg ttttgtcgcc tgttccacct ggcgcgagat
     4321 ccgctcgcca aacccggacc aatccatacc gatgcctgca aaattaccaa aatcctcagc
     4381 cgaatcaccc gcattggact gattggtcac acgaatatcg ccgcctgcgc ttaaggtcac
     4441 caaggcagaa ccatcgccca aggtcaccac acgcgaagtg gcatcgtcat cattgtcaac
     4501 acccttccat tctatgtcga tctcatcggc agaaagggtc aaggttgcat tggctctggg
     4561 aggcatgacc agtagaatat cttcacccgc agtcacagca taggcattac cgggtttcgg
     4621 atcaaggtaa agcactacgt cctcagccac atttgcgctc acatttccac gcacatcgcg
     4681 caaggccaga tcatcggcaa ccgattcaag ggatacgttg ccatccacat cacggatgga
     4741 tacgtcacta agcacttttt tgatggcaat gcttcccttt gcaccgcgca gactgaaatc
     4801 agagtggatc gtaccgatcg aaaccgaatt cacatcacgg atggaaagat cgccttcgat
     4861 ttcgttcaat tcaatatcac ccatcactcc gcgcatggcg gcatcgccgc cgatcttttt
     4921 gatggaaacc gatgccgatt tgggcacacg cagggaaagg tcatcatcac acgaaatgat
     4981 gacttgattc ccatcctgac ggatatgcat ttcatcttca tcacccttca gcaaaatatc
     5041 atcgccttcc cagccgacaa ggctgagatc accttcaata ttctctatga caactctggg
     5101 tgagcgccct gctgaaattg tcttggacat ggcctactcc tcttctccac gcagtagacg
     5161 ggtggcttca tctgccgtga tcttacctgc atgcagatcg tcaagaatac tattccgttt
     5221 ttcctgtgta atttccacgg gttcgtcacg gcctggctcg tatcccaggg cacggatgat
     5281 ctcatgcaat cggttacgga tggtcgggta ggaaagcccg atctcctgtt ccatgcggtt
     5341 gatcttgcct tcacagcgca cgaaggtgaa gacgaattcc agctgttccg gggtcaggtt
     5401 tgaaaactga cccgaagcga aattcccttc gatggtcgta tcacaagacg aacaatgcaa
     5461 tcgtgcaaca gttaattcag actggcagat cggacaacgg gtaggggctg gtcgcatgag
     5521 gattcctcca gggttaggat gccgtaactt caggcatgag ttccaaattt tgtgtttgtt
     5581 ttgatttcac tcgatacagt acaagaaaac cgactgttga caacgtacca acaatacctg
     5641 ctgcatacca cagcatgtgc ggtgagggtc catcgatgat cagaccagca aggtaggggc
     5701 ctactgcaaa gggtatcccc catgaaaagc cggagactgc catgtaacgt ccgcgcattt
     5761 cttcgggagc gaaggacgct acaagcgcct gcgcaactgg tgagaccacc atctcaccga
     5821 aggtaattat aaccatggct gccacgaaca tggcgtaagt ggttacaaat ccgtacatgg
     5881 agaaaccgat cacgtacagg aaggtcccag ccgccatcat taacatgggc gggaagtttg
     5941 tgatgcggcg tgtaattggg aactgcatca acacaaccat ggcagcattg atactgagca
     6001 aaagtccata ccccgattcg ttaatgccgt attcattccg caggaacacg cccagcgatg
     6061 tgttcatatt catataagtg aagacttgca acaacaccgc tccaaggaag agcatgaagg
     6121 ctgtattgcg gaaaacctgc ccatacccgg cgaaggtgcg cgtcatgctt tctttgggtg
     6181 catcagggtg tgcttcgggt ttggtttcag gcaggaaaat ggcgatcagg atcactgtca
     6241 gcaaggaaat ggccgcatcg gacaggaaca gcataaggta agaacgagcc gcaagcagtc
     6301 ccccaatggc agggccgatc accacagaga ggttaaacgc aacgcgcaaa atgccgtatc
     6361 catcagcccg tttatcttca ggcagaatat ctgcgatcat ggcttgatga gcagggccag
     6421 ctacatcggt caatattcca atgaagaaag ccagcacaaa gaaatattca aacgatgtga
     6481 ccagacccat tgccaccgtg ctaaacgagg atgcgatcaa gccaaaaatg atgattccct
     6541 tgcggccaaa tcggtcggtc aatgccccac cgacagcgga accgacaaaa cttgaaacgg
     6601 aaaacgctgc aaaaagcacg ccgacatcgg ccatgcccac cccaaatttg ctggtaagat
     6661 agagcgcaaa aaatggaaat agcaacgcac cgccaatacg atcaataaaa gtaatgacga
     6721 ttaagatcca gaatgtacgc ggatattcgc gatagagttt cgtggctttt tcaatggtgt
     6781 tatttagcat gataagctgc cttctttaca agttgcgtcg aattggcctc gtgcaaggat
     6841 tggttgtagc gtttgcgcag tttctcgccg cgtgagacca tccaatttcc aatccatgca
     6901 aggtttttat ctaaaagggt tttcccttta acggcttctt cttcaaggtg aatggcatcc
     6961 aaatcttcct gaatgctttt gcgtttcaac tcagacatac gctccgataa aatgtccatg
     7021 gaaactcctt ttgcttgtca aaatcaatcg gcttttataa aaatcaaggt gtcacgtcta
     7081 atttctaata aaattataca acttttttga actttgtcaa tagaaaagta aataatatca
     7141 attagtatat tgtaaaaatt gatactttcc tcaaaaaata tcaattttcc atgcttttgg
     7201 aggcaaaata tgattcgttc gctatttttt tcgcccggaa aacctgttcg caccaacatc
     7261 cctactgaag agtttccccg cctcttgcga gaccgccgcg gactgctttg ggtggatttt
     7321 gaggccgaac ccgaagaaag tgcattaccc atccttaatg gttttggatt ccaccaacta
     7381 gccattgacg atgccctgca acaaacccac gcacctaaga tcgatgattg gggagactac
     7441 ctctatatcg ttctaaacta tatgcatctg acaaaagcca ccgaaccctg ggatacggag
     7501 atcgatgagt tggatatctt cctggggcga aactatgtca tcacccacca cgataacccc
     7561 atcacttcca ttaatgaaac ctggaatacc agtcagcgtg acccgcgcta ttcgcaagat
     7621 ggcgccgacc acctgctcta caagatcatt gacgccattg tgatgaatta catgcccata
     7681 attgaaaaaa tagatgaaga gatagattgg gtcgaagacc aggtctttga ccgccccagt
     7741 tcacaaaccc ttgccagatt attcacactc aaacgtgtcc tgcttgccat gcggcgcatt
     7801 ctgctgcccc agcgtgaagt actcaacaaa atggcacgtg acgactttca ggtcattgac
     7861 cgtaaagacc gtatcttttt ccgcgatatt tacgaccacc tcgtccgttt gcatgacgta
     7921 aatgaaagtc tgcgcgatct cgtcggcggc gcattagata cctacctatc cgtcatcaac
     7981 aaccgcatga atgaggtcat gaaaaccctt accatcatca ccaccctttt catgccgttg
     8041 acttttgtaa caggtttttt tgggatgaat ttcttcgccg cgaatcctcc ctacgaaagc
     8101 tggaccctgc cagcggtctt ttacgggacc ctgagcctga tgctgttatc cccaatcatt
     8161 atgttcatct ggatgcgtcg ccatacctgg gtataggcgg ccccatggtt tgccgcataa
     8221 atctgctaaa aaaatccttg acacagaaca ggcgttcggt tatcattcct agcacaaatg
     8281 agcgaaaaat gcgctcgtgg aggctaatat gatgatggtt agaaaaagca aaggtctggg
     8341 cgaacgccac cagaaaatcc ttgatttcat tgcgagctat caacgcgatc acaagcaccc
     8401 cccttcaatt cgggagattg gggagcattg tgacatttcc tctacttctg tagttaatta
     8461 ttatctggat cagctggaaa aatcgggcaa catcgaacgt gatcgcaaaa tatcccgcgg
     8521 tgttcgtctg acaggctcca caccgcttgg tgatatgttc cgcgttccag tgcttggtgt
     8581 cattcaagct ggtgaaccca tccctgtccc ttccaatgct gatttcaatg cctttacccc
     8641 cgaagactcg gttgaaattg ccatgtcttt gatgccaacg aaagaaaaag gcaaggaatt
     8701 gttcgcgcta aaagtccagg gagaatcgat gatcgacgcc atggtaaacg atggtgatat
     8761 cgtcatcctc aaacccaccc aggacgcccg caacggcgac atggttgcgg tatggctttc
     8821 agaccgcaac gaaaccactc tgaaattctt cttcaaggaa aaagacaagt accgcctgca
     8881 acccgccaac cccaccatga gcccgatcta tgtcaacaag aacgaggctc tggagatcaa
     8941 aggcaaggtg gtcatggtca ttcgcaaagt agaacggtac atgtaataaa caaataaaaa
     9001 aggacggctc ttgcgagccg tcctttttta tttaagactt gcttcttact caaccgtgat
     9061 aacggcgcca gcttcttcga gcttcttctt ggcgtccatc gcggcatctt tgccaacagc
     9121 ggagagtacc ttgccgccag cggtttcagc gagggtcttg gcttcgccga ggccgaggct
     9181 ggtcaactga cgaatgacct tgatgacgtc gatcttcttg gcgccagcgt ccttgatgac
     9241 cacatcgaac tcagtcttct cttcaacagg ttcagcggaa gcagcagcgc cgccagcgac
     9301 agcagctaca gccacaggag cggcggcgga aacgccccac ttctcttcga gcatttttac
     9361 gagatcagcc gcctcaagga cggaaagtgt ggaaagttca tccacgagtt ttgcgagctt
     9421 atcagacatt gtatagcact ccttgctaga taatttgaaa tttgattatg gttcgggcag
     9481 ggcccgacga taaatcgata actaggccga agcctgtgcg ctttcagaac gcgccttgat
     9541 gacagccgcg agaccgcgag caggttctgc gagcgtgcgg acgagtttgc cagccggggc
     9601 ctgcaaaacg ccgagcaatg ttgcacgcat aacgggcagc ggcggcatat cagacagggc
     9661 cttgacctgg gcggggctca aagacttgcc gcccatcaaa ccgcccttca ccttgacgaa
     9721 ctcattgcct ttgatggcat ccgtcaatgc ttttgcggtg gaagccgggt ccttgaatgc
     9781 gaacgaaacg gccgtagact tcacaagata atctgcagga acatccatac cattatctgc
     9841 aaaaacacgg cgggcaagtg tattcttgac gatatggaat tcaccgccag tttcacggat
     9901 ctttgagcgg atcgcatcga tcgacttcat atttgcgccg ctatattcca caagaatgac
     9961 cgcttcactg cgcttgaccc aatctgcata ctgggcctgt acttcttcct tgcgttgttt
    10021 ggaaattgcc aaaggttatt cacctccttt caaataaaaa gtctttgcca cttacgcagg
    10081 caaagacttt ttgcaatccc cgcgagggga agccctactg ggagggaaac cggcacagaa
    10141 atatgccgat attttgctat cagtctccac ctgagcgggg aattaagcct tgaagatctc
    10201 aaggcacccg ccttcactgg cgaagtggta ttttaggttt taacgagtat ttctactcgg
    10261 caccatcaat gtgttcattc gggtctgtct tgatgccagg acccatggtt gaagaaatcg
    10321 taatgcgctt gacgaagtta cccttggcac cagatgggcg ggatttgtta actgcatcca
    10381 tcaaagccac atagttttcg aaaagcttat cagcaccaaa agaagccttg ccaattgaaa
    10441 catgcacgtt gttcgtctta tcaaggcggt actcaacacg gccagccttg gcttctttga
    10501 tcgcacgttc catatcagca gcaccaacta cggtaccagc cttgggattc ggcatcaaac
    10561 cgcgaggacc gagaacacga cccaaacgac cgacctttcc cattgcatcg ggtgtggcga
    10621 tggcaacatc gaaatccaac gcaccacctt cgatcttctt catggtctca tcatcatctg
    10681 cgaccagatc ggcaccagca gcacgagccg cagcagcacc ttcaccctgg gcaaatacga
    10741 gcacgcgaac cgtcttacca aggccgtgag ggagcacgac cacatcgcgc aactgctgat
    10801 cagcctgacg agagtcaagt gtcgtgcgca tgtgaacttc gatggtcgca tcgaatttgg
    10861 taatggaagt ttcttttgcc agtgacaccg cttcgcgggc agtgtaaacc ttatcaatat
    10921 caaccttggc agccgccgca gtatattttt ttccgtgttt agccatttta tcctccgtgg
    10981 tacgaacgga cagcacagag ccgtcctccc acaaaattaa tcggttaccg taatgcccat
    11041 cgaacgggct gtaccttcaa cctgcttcat tgcgccttcg atgttgatcg cattcagatc
    11101 cttcatcttc agttcggcaa tttccttcac ctgggcacgg gtcactttac cgaccttgtc
    11161 cttgttcgga acgcctgatc ccttttcaac cttggcagcc ttgcgaagca aaaccgcggc
    11221 gggcggggtg cgcagaacaa atgtgaatga accatctgta taaatggtaa tttctgcagg
    11281 gagtacttca cccggacgat tggaagttcg ggcattgtat tccttacaaa aggccatgat
    11341 gttgatgcca tggctagcga gtgcaggacc gacgggaggc gcagggtttg ccttgccagc
    11401 ctcaagctgc agacgtacga ttgcttttag tttctttgcc atttcgactt aactcctttg
    11461 tggttttagc gggtcatttg ggagaccctc ccaccgcatc aggccaaact gaggcctgct
    11521 actgtccaat taatattttc tactttacgc cttctcaacc tgaagaaaat ccaactcaac
    11581 aggcgtttca cgtccgaaga aactgaccat gacgcgaact tttgtgcgtt ccatgtcaat
    11641 ttctgaaacc acaccacgaa aatcattgaa aggcccgtcc acaatgcgaa cccgctcacc
    11701 cactttgaat gaaactttga ccgtgggagc ttcagcttcc attcgtttaa cgatctgggc
    11761 aacttcttca gggcgcaagg gtgtggggct gttccccatt ccaacaaaac cagtgacgcc
    11821 aggggtgtta cgcaccacat accaggattc ttcggagagg gtcaaattca ccaacacata
    11881 accagggaag atgtgacgtt ccacagtgcg gcgttttccg tctttgactt cgatttcttc
    11941 ctgagtcgga ataaccacgt caaaaatctt gtctttcatt cccatggtct cgatgcgttg
    12001 ctcgagatta tggcgaacct tgttctcgta tccagaataa caatggatca cataccaagc
    12061 aggcccctcg acaattgtct caaccggggg caggtcggtg ggaatctgcg attcggaggc
    12121 aggaacaggt ttcgtgtccg aggaagaaac ggaaacaacc gcaggagtat tctgctcctc
    12181 ggtttgctca cccatcggtt cctgttcaaa ctgctcttgc gattccggta aatccatcat
    12241 tttcctctat tcaagactaa attccgagta tcagatttat caataagtcc gacacatagt
    12301 caatgattgc caggaaaaaa cccacaatca ccataaccgc caaaacgagt tctgtgagac
    12361 gccttgcttc tggccaggta ggccagctga ctttgcgaag ttcaccagat gtttcgcgga
    12421 aataacgctg aacggcgtta tcactcttct tgactttctt ctctttctca gccaaggtaa
    12481 tactccttct atggttttac ggctaaaacg ccgcacaaag cgcggcgttt gccacaggca
    12541 ggccaggtag gaatcgaacc cacaaccgct cgttttggag acgagtgctc tgccaattga
    12601 gccactggcc taaattagtc agatggccgc tttgtatcag ctacttatca gggcagcctt
    12661 tgctccacgg tcatctgacg atgaaactac ttggtttcgc ggtgctgggt gtgcttcttg
    12721 cagcgcgagc aatacttgtt aaactccaac cgattcggat cattgcggcg attcttttcg
    12781 gtggtgtagt tccgctcttt gcagtcactg cactgaagtg taatgaccgg gcggatgtct
    12841 ttcttcttcg aagccatctt gctaccttac tttcctgaat tactcgatga tcttggtaac
    12901 gacacccgcg ccgacggtga gaccgccttc acgaatggcg aacttggaac cctgctcaag
    12961 agccacaggc acaatcaact ctacggtcag gttcacattg tcaccaggca tgaccatttc
    13021 cacaccttcg ggcaacttga tgtcacctgt cacatccatc gtgcggacat agaactgcgg
    13081 acgatatcca ctgaagaacg ccttgtgacg tccgccttct tccttcttca acacgtacac
    13141 ttccgcaagg aacttcttgt gcggggtgat cgacttcggc ttggccaata cctgaccacg
    13201 ctccacatct tcacgttcaa taccacgcaa caacagacct acgttgtcac ctgccatacc
    13261 ttcgtccaac tgcttgtgga acatttccac acctgtgcag acggtgctct tggtttcgcg
    13321 aagtccaacg atttcgatcg gctcacctac tttgacaacg ccacggtcaa tacgtccagt
    13381 taccactgta ccgcgaccct tgatcgagaa tacgtcttcc acgctcatca tgaacggctt
    13441 gtccgtatca cgcttcggct caggaatgta ttcgtcaatc acgcgcaaca actcgcggat
    13501 cggggcatat tcgggggcgt tcgggtcagt cgctgcactt tccaaggcct gcttcgctga
    13561 tccgcggacg atcggggtgg tgtcaccggg gaagccctgc ttgttcagca gttcgcgcag
    13621 ttccaactcc accagttcga gcagttcggg gtcatccatc atgtccactt tgttcaggaa
    13681 cacaacgatt gacggaactt ccacctggcg tgccaacagc acatgttcgt gtgtctgggg
    13741 cattggacca tcgggggctg ccactaccag aatcgctccg tccacctgag ccgcaccggt
    13801 gatcatgttc ttgatgtagt cgcggtgacc gggcatgtcc acgtgtgcat agtggcgctt
    13861 gtcggtctgg tattccacgt gtgcaatgtt gatcgtgata ccgcgcgctt tttcttcagg
    13921 tgcgttgtca atctggtcgt aggctttgaa ttctgcctgg cccgccagac ccgctacttt
    13981 tgtgattgcc gctgtcaatg tcgtcttccc atggtcaatg tgtcccatgg tccctacatt
    14041 tagatgcggt ttgctgcgat caaatttttc cttcgccat
//
LOCUS       DAOQLC010000109        14061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1599_length_14061_cov_16.269385, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000109 DAOQLC010000000
VERSION     DAOQLC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 14061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1599_length_14061_cov_16.269385"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PL000_16370"
     CDS             <1..420
                     /locus_tag="PL000_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90508.1"
                     /translation="VLDSLTELSNACMKMIIGSKPTASQPEYGVAQNNLINFLRLLTQ
                     ALPATFVMTAHVDRQMDEITGTVKLMVKSIGKALAGDIPPLFSDVILTTREATKFYWD
                     TAAANADTKTRSLPIQGKIEPNFGQIMNTWANRANVK"
     gene            432..653
                     /locus_tag="PL000_16375"
     CDS             432..653
                     /locus_tag="PL000_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90509.1"
                     /translation="MDETQNEVKSVRSATDAEVLLHNALQNKWPFVLCVKTPTARGTV
                     TNLSEKEEAQMLAKFARRAKKRLQASEGL"
     gene            653..1078
                     /locus_tag="PL000_16380"
     CDS             653..1078
                     /locus_tag="PL000_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90510.1"
                     /translation="MPTAQTFLESAGKLLQDRGHQYDRPEGERSMGAAVQAFNIITKH
                     NLSEAEGWLLLQILKDVRQWQNPDYHRDSAEDCVAYAALKAEALSAQQKTGLYSPDSE
                     KVNVQNEHQAFVSALSGGVYPKVPGTLHDGKFVPRLHED"
     gene            1142..1603
                     /locus_tag="PL000_16385"
     CDS             1142..1603
                     /locus_tag="PL000_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90511.1"
                     /translation="MAQLFSAETYINAVMDQPLERRIPLPPNDYVGIIQKVEARQWSS
                     KSDPSKSGVALDISIQLDLPFEVQQECQMDKPLFTIRDSVMLDLTENGALAQGPGQNR
                     RLRGYREATHKNQLGVPFSPKDLVGCLIKVRIVHDLYEDQPIEKITAVTEA"
     gene            1673..2011
                     /locus_tag="PL000_16390"
     CDS             1673..2011
                     /locus_tag="PL000_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90512.1"
                     /translation="METKTAYVAYTNTDLTAGQGYQIPAALCAIRATAVRLAKGINVQ
                     GSDGPVKEVSLVKVDGMWYAPIGTAVRLEQPLTKDLDAQKEYDKRVAVIEKARAAGLT
                     EEDLASLKAL"
     gene            2141..3463
                     /locus_tag="PL000_16395"
     CDS             2141..3463
                     /locus_tag="PL000_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNC90513.1"
                     /translation="MNTYCDISVIQLAENRQRRLFAEEALMELQDSIERIGLMHPIVL
                     RQTEDSVVLVAGERRLRAIQNIYGLGGSFTFNGQTVPAGQIPFVNLGDLTSIEAQEAE
                     LEENTRRVDLTWQERAQATAALVALRRAQADNLDKLPPTLGDLAEELKGTRGNSSKEA
                     VSKELIVARHLDNPAVRDAASVKDAFKALKRVEQADQHKKIAKELGSVLMTSKYFLEQ
                     ADCLQWMQGKKEQFDVICTDPPYGIGADTFGSAGKTDATYSAHGYDDSPGNFRAIMGE
                     FPELAYNIAKPDAHLYMFCDFSYFRVLYALFSDAGWNVFRTPLIWFKPQAFRAPWPEH
                     GPQRKYEMILYARKGDKKTTKIAGDVLTYPADEQLGHSAQKPVGLIRDLLSRSVIPGN
                     TVLDPFCGTGTIFKAAQDLPCIVTGVELDQQFYGIAAKRIQELSGEKK"
     gene            3460..4134
                     /locus_tag="PL000_16400"
     CDS             3460..4134
                     /locus_tag="PL000_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC90514.1"
                     /translation="MTTILLDTETNGREPQEPIEVAWVELEEGPAFFLNRKTNIHIDR
                     YKPELISTYGALATHHIMPEELRKCKPSSEAIIPPTDYLIGHNVDFDWQVMGQPPCKR
                     ICTLAMARVVYPRVDSYSLVALSYYLRGANLLVKDVVKNAHCALDDVALCHWILQAIV
                     DERYITDMESLYVFSEKCRIPEIISFGKHKGTPIWQLPKSYVQWLLRQPDLDPYLQKA
                     IEKYCR"
     gene            4185..6608
                     /locus_tag="PL000_16405"
     CDS             4185..6608
                     /locus_tag="PL000_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNC90515.1"
                     /translation="MILGSGNQNATIMIVGDCFTPLEATSVEPFLGENGRALNAVLHE
                     AKILRSQCYLTNVYNAVPPAGDMCYIIPKEKKHIHAEMVSWRGKQVHRRLLAEVIRLE
                     REIELVRPNVIVAAGDEALWVLTGAVGVDKWRGSLLTLDGEPGKTKVIPIYHPSRVAW
                     VADMKALTVQDLRRVAQESKTPELHEPKWNYLVEPFYHTVIEALNEILGRLNGNYELV
                     WLTLDLETSVGHIACCGLATDPLNAICIPFMCQEYKNGYWSLLEEIEIVSLLHKVLTH
                     PNVRVRGQNLLYDCQYIYRHWKFVPRVAQDTMITHHTIWAGLPKRLDFQASMYCEFYR
                     YWKDDGKTWNTNISDAQWWRYNCMDCTYTHEIGEVTQAAVKKLGLGAVEAFQQAMFWP
                     ILKTMLTGVRIDKTRQSKLAIDLLDEIACRETYINTVAGHSLNIRSSQQMCKFFYDDL
                     QIPPIKSPPKRGQVSHITCNSEALQKIKVIQPILTPLINKIDELRSLGVFHSTFVRAG
                     LDLDGRMRCSYNPCGTETYRLSSSKSAFNTGTNLQNIPSGSEEEGGLQLPNIRKLFIP
                     DSGFTFFDMDQDRADLQVVVWEANDAELKDALRRGVDMHLFNAINLAGGTSPDIDWLV
                     KGHPEYDRILARYVRERRLAKPFIHGTNYGGKPVTMAKAAGITTHQADMFQRRYFARH
                     PGIVAWHERTKAFLDTRKYVENQFGYRRFYFEDTGSAFTKALAWIPQSTVALVVNRIW
                     MNFFNNLPEVMVLLQVHDSLAGQFPTIQRDYCLKRMKEEARIVIPYEDPLVIPVGLKT
                     SLTSWGDCK"
     gene            6739..8010
                     /locus_tag="PL000_16410"
     CDS             6739..8010
                     /locus_tag="PL000_16410"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNC90516.1"
                     /translation="MARNFPSWIDAYVKYAGVTEAPRRMAFWAGVSAVAGALRRHVWI
                     DMKRFQWTPNFYIIFVAPPGIVAKSTSADIAMDLLKQVPGIKFGPDTVTWPALVTAFA
                     NAQESFMMDETWYPMSPLTLVSSEMGSLINPMDRQMVDLYITLWDGRKGYEKVTKMSG
                     NDTISAPWINMLACTTPQWLADNMPAATIGGGFTSRCIFLYSDSKERYIPFVDEMVDD
                     NDEDTRDKLIQDLEHISVNLVGPYNITKEARDWYRPVYEEFWKNVHLRMDDSMMEGYA
                     ARKQTHLFKTALILSAAQRDEKVITLDDLQLASLMLDDVESTMGKVFSRIGKSDDSLA
                     AEKFIQHVKKNGELSYEQAYRMVHAYFPDFRNFEGMVSGAVKSGYLILIQKSTGLFLR
                     AKEETPSQPPAPISREEAYQNELLERQMAGR"
     gene            complement(8252..8491)
                     /locus_tag="PL000_16415"
     CDS             complement(8252..8491)
                     /locus_tag="PL000_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90517.1"
                     /translation="MSDRHLNEYNKAVGEGAYPASPAPPTAPGGSANPTIYKDMDQFS
                     QSVRAMVARTGMTPDAAKRFIIDDWGKKGIVLKPY"
     gene            complement(8709..8993)
                     /locus_tag="PL000_16420"
     CDS             complement(8709..8993)
                     /locus_tag="PL000_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90518.1"
                     /translation="MANEQEEVCPPYERRLAWQEESAKLHARIDEITGMLQDMRRILD
                     VHVALDKDISPALKEIVVVWKGSKIMIPLLVTILTGLVASYAWIKDHIKP"
     gene            complement(9006..>9212)
                     /locus_tag="PL000_16425"
     CDS             complement(9006..>9212)
                     /locus_tag="PL000_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90519.1"
                     /translation="DTENNLTNATAKLIALKNAATEKFSITAAGMLNMVVPANYANDA
                     AAAAGGVPVGGVYRNGSVLQIRVT"
     assembly_gap    9213..9312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9313..9953)
                     /locus_tag="PL000_16430"
     CDS             complement(<9313..9953)
                     /locus_tag="PL000_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90520.1"
                     /translation="MSIFNLPPPPATSDVTALSWRDWFYKLTIALKTFVASALPAGGT
                     TGQYLKKNSNADQDAGWVTPTAILPSGGTTGQALTKNSNADQDAGWSTISIDLTNYAY
                     LPGRAGGQTLKGGTGTTDLLNLVGTAGNGVATNAAIALKVGNAGATTAMTVYNDTAIS
                     LGSAAKEDPTLFALAANKGIKVNFASPSSDSHCFEATSSRHCFYAQATGVGTG"
     gene            complement(9950..11260)
                     /locus_tag="PL000_16435"
     CDS             complement(9950..11260)
                     /locus_tag="PL000_16435"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90521.1"
                     /translation="MKILIIDKDSCGLGMALKFQEAHHQVKLFISPASKSCKAGLGLV
                     PKVEAWQPHAKWADMIVMLDNSYAMEDLERLIRQGYPVFGSNLEGATWELDRKKGQKI
                     LQSAGIQTIPGQSFTRYEDAEAYVLGRPKRYVSKPNGDMDKKLSYVAKNAADLIYMLR
                     SWKRQGKIQSEFIIQDFLPGIEMGVGGWFGPDGWSKALCENWEFKKLCNDDLGVATGE
                     MGTVLRYVEKSALAEEVLLPLTPYLHAINYVGYCDVNCIISEEGIPFPLEFTMRFGWP
                     LANIQMAVHRGDPAQWMLDKIEGRDTLRVSDEIALGVVMVLPDFPYSSYTARVLEGIP
                     IYGITKSSARHISLSSVMAGKAPAMEGGRVVEKALPVSAGDYLCVVTTSGDTVSEARD
                     STYKIVKSLEVPNSIQYRTDIGCRLEKQLPTLHDYGFATGMKYK"
     gene            complement(11263..13971)
                     /locus_tag="PL000_16440"
     CDS             complement(11263..13971)
                     /locus_tag="PL000_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90522.1"
                     /translation="MEWEQKIKNRPEIYGDERIRINQEKLQELRETIAEYQNSPPFRP
                     EHQSIYNRYNRDIASYLKTLGAKPYTDQHGHGWLEVSIDKAREQTGGPRTNMYGQIDP
                     RLAATMGTVAGGALVGAYLADDRNKFFGLLTGAAGAALGLRLLKPLVEKLPPSLGTKE
                     AYVKDAVDRLGPQYEQAARFHWDQLQKAITTDPKVGGKTVTEQAESLSDGQYQLLKQR
                     EATMIRTKTYFEQLSPEVRKIASSERMYDAQEGEVPGAANRLTPEEQAVKASILDPLT
                     RRLEALYREAVRVNEGKEIADLDPHNPRMTKRRWMDKVVSFGEGAFPGSGFVRQAGSM
                     KGRSMFGLQMPDGSLRVVNQQGRDLIGYGPDRNPIGVIARSGKELKVGENVTVAGGTA
                     KLVQVSTRMIESQTKLEYSKNALANKLAAVAELESYIRERKWMGETLKALTDQGYAIK
                     ADSAAGIPKGFRRVLGDRALEQYYIQNRLANTFQDGVLKSGSSTNLLERINSAAVGTM
                     FWNPYPHLFNAADHLIDSVGWGWFDPRQLQTLGKEFYQAYKDVSTLSPDYQKYLNSGM
                     ALQYAAVVTRDTVANLIKGIPERGWKAMAEEYGYGARPDRMIADIFDKSRRILWGGSD
                     VMMITAYKHLASKKGTDILNQAIRRHVEAHNPTYRVPSNIGFDELMKIPNMPEAVAES
                     ISRFASRAMQSRIGNLFGRYHYGQMKSFGHLMRDNLVQDAKSIETRGEAQAHLAFSAF
                     KLFVVYPYMLDSIAKYLSGDPDAEMRRAGATTIPNVFYHIILGDKKVNDLLKEAWSLP
                     PATKMIAELDANRQMFTGKHIWEPGDNALGAANDVGLWAVGSMISPARDAMDISSGHK
                     SGKQFALGAIGIKSKTPEQKAKTEAYKAREASEARRRQLQRGYLKD"
BASE COUNT         3361 a         3826 c         3494 g         3280 t
ORIGIN      
        1 gttctcgact ccctcacgga gctgtccaac gcctgcatga aaatgattat tgggagcaag
       61 ccgaccgcat cgcagcctga atacggcgta gcccagaaca acctcatcaa ctttctccgc
      121 ctgctgaccc aggctctccc tgcaaccttc gtcatgactg cgcacgttga taggcagatg
      181 gacgaaatca ccggaaccgt caagctcatg gtcaagtcga tcggcaaggc cctggctggc
      241 gacattcccc ctctgttttc cgacgtgatc ctgaccacac gcgaagccac caagttttat
      301 tgggacaccg ccgcagccaa tgcggacacg aagaccagaa gtcttcccat ccaaggaaaa
      361 attgagccca atttcgggca aatcatgaac acctgggcta accgggcgaa cgttaagtaa
      421 ggggaagtga gatggatgaa acacaaaatg aagttaaatc tgttcgttcg gcaactgatg
      481 cggaagtatt gctacacaat gctctgcaaa acaagtggcc ctttgtactg tgtgtgaaaa
      541 ccccaactgc ccggggcaca gtaacaaacc tgagcgagaa ggaagaagcc caaatgctgg
      601 ccaagttcgc caggagggct aagaagcggc ttcaagcgtc ggagggacta taatgcctac
      661 cgcacagaca tttctcgaat ccgccggcaa gctcctccag gatcgcggcc accaatacga
      721 tcgtcctgag ggagaacgga gcatgggtgc cgctgttcag gctttcaaca tcattaccaa
      781 gcacaacctt tctgaggccg aaggctggct attactgcaa atcctcaagg atgttcggca
      841 gtggcaaaat cccgactacc accgggactc cgcagaagat tgcgtggcct atgcggcgct
      901 gaaggcggag gcattaagtg cccaacaaaa gacaggactt tattcccccg attcggaaaa
      961 agtaaacgta cagaatgaac accaagcatt cgtctccgct ctttctggcg gtgtctatcc
     1021 aaaagttccg gggactttac atgacggcaa gttcgttccc cgtcttcacg aagattgaag
     1081 aaaaagcccg caggagcttt aatcctgcac cctccatcca tccttatcaa ggagtttcaa
     1141 aatggcacaa ctgttttccg cagaaaccta catcaatgca gtcatggatc agcccctgga
     1201 acgtcgcatc cccctgccgc cgaacgacta cgtgggcatc atccagaagg tcgaagcccg
     1261 gcaatggtcc tccaagtccg atccgtcgaa atccggcgtc gccctggaca tttccattca
     1321 gcttgacctg cccttcgagg tccagcaaga atgccagatg gacaagcccc ttttcaccat
     1381 ccgggacagc gtcatgctgg atctgacgga aaatggggca ttggcgcagg ggccagggca
     1441 gaaccgccgg ctccggggct accgcgaggc aacccacaag aaccagctcg gcgttccctt
     1501 ctccccgaag gatctggtgg gatgcctgat caaggttcgg atagtgcatg atctgtacga
     1561 ggatcagccg atcgaaaaga tcacggcggt aacggaagct taattgttgt tgggggggtc
     1621 gttgttaggc ccttccctct tttacctgga aggaaaattg agaggaggaa cgatggaaac
     1681 aaagacagca tacgtggctt acacaaacac ggaccttacc gcagggcagg gttatcaaat
     1741 cccggcagct ttatgcgcca ttcgtgcgac agctgtaagg ctcgctaaag ggataaatgt
     1801 ccaggggtca gacgggccgg ttaaggaagt gagtctagtg aaagtggacg gtatgtggta
     1861 tgctccgatt ggaactgcag ttcgcttgga acaaccacta acaaaagatc tagatgcgca
     1921 gaaggaatac gacaaacggg ttgcagtgat cgagaaagcg cgcgcggcgg gtttgaccga
     1981 agaagacttg gcctcactta aagctctatg agagggaaac aactacctcg cgccgtgcgg
     2041 gtcgatctgc gaaaagtgcg ggcaggcgac gtgaacaaca acgaaccttt atgtgaatca
     2101 tgttacacca gtttaattga acacccatca ggagattgaa atgaacacct attgcgacat
     2161 atccgtcatc caactggcag aaaaccgtca gcgccgcctt ttcgctgaag aagctctgat
     2221 ggaactccaa gactccatcg aacgcatcgg cctgatgcac cccatcgtcc tgcggcagac
     2281 agaggattct gtcgtgctgg tcgccgggga gcgtcgcctt cgcgccatcc agaacatcta
     2341 tggtctcggc ggttccttta ctttcaacgg gcagactgtt cctgcaggcc agatcccctt
     2401 tgtcaacctc ggcgacctca ctagcatcga agcccaagaa gctgaactgg aggaaaacac
     2461 ccggcgggtc gatcttacct ggcaggaacg tgctcaggcc actgctgccc ttgttgccct
     2521 tcgccgcgct caggcagaca atctcgataa gctccccccg actctggggg atttggcaga
     2581 agagctgaag ggtactcggg ggaactccag caaagaggcg gtgtccaagg agctaatcgt
     2641 agctcgccac ctggacaacc ctgctgttcg cgacgcagca agcgtgaagg atgcctttaa
     2701 ggcactgaaa cgagtcgagc aggccgacca gcataaaaag attgcgaagg agctgggatc
     2761 tgttctgatg acctcgaagt attttctgga acaagcagac tgccttcaat ggatgcaggg
     2821 gaaaaaagaa cagttcgacg tgatctgtac tgatcccccc tacggcatag gggcagatac
     2881 ctttgggtcg gcggggaaaa ctgatgccac ctacagcgct cacgggtacg acgactcgcc
     2941 cggaaatttt cgcgctatca tgggggagtt tcccgaactg gcctacaaca ttgcaaagcc
     3001 agatgcccat ctgtatatgt tctgcgattt ctcctatttt cgcgttcttt atgccctttt
     3061 cagcgacgcc ggctggaacg tctttcgcac cccgcttatt tggttcaagc ctcaagcctt
     3121 ccgcgcccct tggccagaac atggtccgca gcgtaaatat gaaatgatcc tgtacgccag
     3181 gaagggagat aagaagacca ccaagattgc cggggacgtg ctaacatacc ctgccgacga
     3241 acaactgggt cacagcgccc agaagcctgt agggctgatc cgcgacctat tgtccaggtc
     3301 tgtaattcct ggcaacaccg tcctcgaccc tttctgcgga acaggtacta tcttcaaggc
     3361 tgcccaggac ctaccctgca tcgttacggg ggttgagctg gatcagcaat tctacggcat
     3421 cgccgcaaag cgtattcaag aactctcagg agaaaagaaa tgaccaccat attgctggac
     3481 acagaaacca acggccggga gccacaagaa cccattgagg ttgcttgggt agaactggaa
     3541 gaagggcctg ccttttttct gaataggaag acaaatatcc acatcgatcg gtataagccg
     3601 gaattgatca gtacttacgg tgcccttgcc acccaccaca tcatgccaga agagctgcgg
     3661 aagtgcaagc ctagcagcga agccatcatc ccccctactg actacctcat cggccataat
     3721 gtagacttcg attggcaggt tatgggtcag ccgccttgca agaggatttg cactcttgcg
     3781 atggccaggg tcgtctatcc tcgcgtcgat agctatagcc tcgtggccct cagttactat
     3841 cttcggggtg ctaatcttct ggtaaaagat gtagtcaaaa atgcccattg cgcactggac
     3901 gatgtggcgc tgtgccattg gattctgcag gctatagtag acgaacgtta cataaccgat
     3961 atggaaagtc tctacgtctt cagcgaaaaa tgccgcattc ccgaaatcat ctcttttggt
     4021 aagcacaaag gtacacccat ctggcaactt cccaagtcct acgtgcaatg gcttctcagg
     4081 cagcctgacc ttgatcccta tctgcagaaa gccatcgaga agtattgccg ttaaccttcc
     4141 taatctaggc ccttttcgca gggccttttc caggagtaca gattatgatt ttaggatcag
     4201 gtaaccagaa cgcaacaatc atgattgtgg gggattgctt tactcccctg gaagcaacat
     4261 cggtagagcc tttcctcggg gaaaacggac gtgcccttaa tgctgttttg cacgaggcca
     4321 aaattctccg cagtcaatgc tacctgacga acgtgtataa tgcagtacct cctgcagggg
     4381 acatgtgcta tatcattccg aaggagaaaa agcacattca cgctgagatg gtttcctgga
     4441 gaggaaagca agttcatcga cggttgcttg cagaggttat tagactagag agggaaattg
     4501 agcttgtccg tccaaacgtg attgttgctg cgggggacga agctctctgg gtgctgacgg
     4561 gggcagtcgg cgtagacaag tggaggggga gtttgttaac gctggatggg gaaccaggta
     4621 agacaaaagt catcccaatc taccacccca gtcgagttgc ctgggtagca gacatgaagg
     4681 cgctgactgt gcaggattta aggcgggttg cacaagaatc taaaacccca gaactacatg
     4741 aacccaaatg gaattatctt gtcgagcctt tttatcatac tgtcatagaa gcccttaatg
     4801 aaattttagg taggctgaat ggcaactacg aacttgtttg gttaacactc gacctcgaaa
     4861 ctagtgtagg tcatatcgct tgttgcgggc tggcaactga cccgttgaat gcaatctgca
     4921 tccccttcat gtgccaggaa tacaagaatg ggtattggtc cttgctggag gaaatcgaaa
     4981 tcgtctccct cctccacaaa gtccttaccc accccaatgt cagagtccgc gggcagaacc
     5041 ttctctatga ctgtcaatac atctaccgtc attggaaatt tgtcccgcgc gtcgctcagg
     5101 acacaatgat aacccaccac acgatctggg caggtcttcc caagcgcctc gatttccaag
     5161 cttccatgta ttgcgagttt tatcgatact ggaaggacga cggaaagacc tggaatacca
     5221 acatcagcga cgcacagtgg tggcgatata actgtatgga ttgcacctac acccatgaaa
     5281 tcggggaggt cacgcaggcg gcggtcaaga agcttggtct gggggcggta gaagccttcc
     5341 agcaagccat gttctggccc attctgaaga ccatgctgac cggcgttcgt attgacaaga
     5401 ctcggcagag caagctggcc attgaccttc tcgacgagat tgcttgcagg gaaacctata
     5461 tcaacaccgt cgcagggcac tcgttgaata tcagatcttc tcagcagatg tgcaaattct
     5521 tctacgacga tctccaaatc ccaccaatta aatctcctcc gaaacgcggg caggtttccc
     5581 acataacttg taacagcgag gccctgcaga agatcaaggt tattcagcct atcctgaccc
     5641 ctctcattaa caagattgac gagcttcgat cccttggggt gtttcattct acctttgtcc
     5701 gagctgggct tgatctggat gggcggatgc ggtgcagcta taatccttgc ggaacagaaa
     5761 cttatcgact gagttccagc aaaagtgcct tcaacaccgg gacgaatctg cagaacattc
     5821 cctcgggatc ggaggaggaa ggtggccttc agcttcccaa catccgtaaa ctatttatcc
     5881 ccgactcagg ctttaccttc ttcgacatgg accaggacag ggcagacctt caagttgttg
     5941 tgtgggaggc gaatgatgca gaactgaaag atgctctgcg ccgtggcgtt gacatgcacc
     6001 tgttcaatgc aatcaacctg gcaggaggga caagccccga catagattgg ctggttaagg
     6061 ggcaccctga atacgacaga atactcgccc gatatgtacg ggaacgccgt ctggccaaac
     6121 ccttcatcca cggaactaac tacggtggta agcccgtaac aatggccaag gcagcgggga
     6181 tcacaacgca ccaagcggat atgtttcaac gccggtattt cgctaggcat cctgggatcg
     6241 tcgcttggca tgagaggact aaggccttcc tggatacccg caagtatgta gagaatcaat
     6301 tcggctatcg tcgattctat ttcgaggaca cagggagcgc ctttaccaaa gcccttgcct
     6361 ggattccgca atcgaccgtc gctctggtag taaacaggat ctggatgaat ttcttcaaca
     6421 accttcctga ggttatggtt ttgctgcaag ttcatgactc gctggcgggg cagttcccta
     6481 cgatccagag agattactgc ttaaagagga tgaaggaaga agccaggatt gttatccctt
     6541 acgaggatcc cctggtgatt ccagtagggc ttaagacttc gctgacgagt tggggagatt
     6601 gcaaatgaaa ccgcatctct acagaaaagg tggctattgg ttctgccgac ttcccggtac
     6661 ctacggagtt gttggaggcc ttgcaacccc cctagaagcc tatcagcagt ataaacttta
     6721 cacgaggata aaaatctgat ggcacgaaac tttccctcct ggatcgacgc ttacgtcaaa
     6781 tacgctggcg ttacagaggc tcctcgccgc atggccttct gggctggtgt gtccgcagtt
     6841 gccggtgccc ttcgacgaca cgtctggatt gatatgaaac gatttcaatg gacacctaac
     6901 ttctatatca tcttcgtagc tccgccaggg attgtagcga agtcaacttc tgccgacata
     6961 gctatggacc tcctcaaaca agtccccggt atcaagttcg gcccggacac cgtaacatgg
     7021 cctgccctgg taactgcctt tgccaacgcc caggagtctt tcatgatgga tgagacctgg
     7081 tatcccatgt ccccactcac cttggtatca tcagaaatgg gcagcctcat caaccctatg
     7141 gacaggcaaa tggtggacct gtacataacc ctctgggacg gtcgtaaggg ctatgagaag
     7201 gttacgaaaa tgtcaggcaa cgacaccatc tctgctccct ggatcaacat gctggcctgc
     7261 accacgcccc aatggctggc agacaacatg cctgcagcta ccatcggagg aggctttaca
     7321 tcgcgctgca tctttctcta ttcagactca aaagaacgct atatcccctt cgtcgatgaa
     7381 atggtagatg acaatgatga ggacaccagg gacaagctca ttcaagactt ggaacacatc
     7441 tctgtgaacc tagtcggccc ctacaacatc acaaaggaag cacgggattg gtatcggccc
     7501 gtctatgaag agttctggaa aaacgtccac cttcgcatgg acgattccat gatggaaggg
     7561 tacgcagcgc ggaagcaaac acacctgttc aagacagccc ttatcctctc cgccgctcag
     7621 agagatgaga aggttattac cctcgacgat cttcagcttg cttccctaat gctggacgat
     7681 gtagagagca caatggggaa ggtattttcc cggattggaa agtccgacga ttcgctggca
     7741 gcggagaagt tcatccagca tgttaagaag aatggggaac tttcctacga acaagcctat
     7801 cggatggtcc atgcctactt ccctgacttc agaaacttcg aggggatggt atcgggagca
     7861 gttaaatctg gttacctcat cctgattcag aagtccacgg gcctattcct tcgtgccaag
     7921 gaagaaaccc caagccagcc accggccccc atctccaggg aagaagccta ccagaacgag
     7981 cttcttgagc gtcaaatggc tggtcggtga gattgtatgt ccacatcaca ttgaggttat
     8041 acggccatga aacgcataac aacagaaagc ctcattacag gccctcgtcc ttctctccat
     8101 tattgctgcc tttgggcctt cttctccctc ttcaaatccc cgacactaat agctgcccgt
     8161 ctcggcgttt gtactaagtc cgtcagtaac gcaaagaaaa gggcaatgca ggaaggttgc
     8221 caacattgcc ctaattgccg gaaagatcag ctcagtaagg tttcaataca attcccttct
     8281 ttccccagtc gtcaatgatg aaccgcttgg ctgcatcagg ggtcatgcca gttctcgcca
     8341 ccattgccct gacggattgg gagaactggt ccatatcctt atagattgtg ggattggcac
     8401 tccccccagg ggctgtaggt ggtgcggggc tggcaggata agctccctcc cctacagcct
     8461 tgttgtattc attcagatgc ctatctgaca tcccccccgc accttgtcgt ccgaacgcat
     8521 cccgcaagag ggttgcattg ttagcctgcg cgagttggaa gctttctgcc aaatccgcat
     8581 cctgttgtaa ttgccccgtt gccctgttga gctggtccca gggcatggtc caaggttcgt
     8641 tcccgccata cgggacagta gtgtacatgt tagctggaag tgccattacc aaactccaaa
     8701 aaagaagact acggtttaat gtgatctttg atccaggcat aggaagctac gaggcccgtc
     8761 agaatggtta ctaataaggg aatcattatc ttgcttccct tccagacaac tacaatttcc
     8821 ttgagggcag gacttatgtc cttatcaaga gctacatgaa cgtctagaat gcggcgcatg
     8881 tcttgcagca taccggtaat ttcatctatc cgtgcatgaa gtttggctga ttcctcctgc
     8941 caagctagtc tgcgttcgta tggcgggcat acttcttctt gttcatttgc cattattttt
     9001 ccttgttagg ttactcgaat ttgaaggaca ctgccattgc ggtaaacccc accaacagga
     9061 acgcccccag ccgctgccgc tgcatcatta gcatagtttg cggggaccac catattcagc
     9121 atccctgcag ctgttatact gaatttttcg gtagcagcat ttttgagtgc tataagtttt
     9181 gcggttgcgt tggtcaggtt gttttccgta tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnatgcctgt tcctacaccg gtagcttgag cgtaaaaaca atgtctgctg
     9361 gaagtagcct caaaacagtg agagtccgaa gaagggctag caaaatttac tttaatgcct
     9421 ttgttagctg ccagagcgaa aagagtggga tcttcttttg cggcacttcc tagtgaaata
     9481 gctgtatcat tgtacacagt catcgccgta gttgcacctg cgttacccac tttaagcgca
     9541 atcgcagcat tagtggcaac cccatttccc gctgtaccaa ctaaatttaa aagatcggtt
     9601 gtccctgtac cacccttcaa ggtttgacca ccagccctcc caggcaaata ggcataattt
     9661 gtcagatcaa ttgatatagt agaccacccc gcatcctgat ccgcattgct attttttgta
     9721 agagcttgac ctgttgtacc ccctgaagga agaatagccg ttggagtaac ccaccctgca
     9781 tcttggtctg cattgctatt tttctttaag tattgccctg tcgttcctcc ggcgggcaga
     9841 gcgctggcta caaaagtctt taaggcgatg gtcagcttat agaaccagtc cctccaggac
     9901 agggccgtta catcgctagt tgcaggaggt ggtgggagat taaagatgct catttgtatt
     9961 tcatcccggt agcgaaccca tagtcgtgga gcgtagggag ttgtttctcc aacctgcacc
    10021 caatgtccgt gcgatactga atgctgttgg gaacttctag agatttgacg atcttatagg
    10081 tagaatccct cgcctcacta accgtatcac ccgaagtcgt caccacacag aggtagtctc
    10141 cggcacttac aggcagggcc ttttccacaa ccctgccgcc ttccattgca ggcgccttgc
    10201 ctgccatcac ggaggacagg gaaatgtgcc gcgccgacga tttggtaatg ccgtagatcg
    10261 ggatcccctc cagcaccctt gccgtatagc tggaatacgg aaagtccggc agcaccatca
    10321 ccaccccgag cgcgatttca tctgaaaccc gtagggtgtc cctaccctca atcttgtcca
    10381 gcatccattg cgcaggatca cctcgatgga cggccatctg aatgtttgcc agcggccatc
    10441 cgaagcgcat tgtaaactcc agagggaagg ggattccttc ttcggaaatg atacagttga
    10501 catcgcaata ccccacgtag ttaatagcat gaaggtaagg ggtgagaggc aggagaacct
    10561 cctcggccag cgcagacttc tctacgtacc gcagaaccgt acccatctcc ccggttgcaa
    10621 ctcccagatc gtcattacaa agtttcttga actcccagtt ttcacagaga gccttggacc
    10681 agccatccgg cccgaaccac ccgccaactc ccatttcgat tccggggaga aagtcctgga
    10741 tgatgaactc ggattggatt ttcccctgcc tcttccagga ccgcagcata tagatcagat
    10801 ccgctgcatt cttggcgacg taggagagtt tcttgtccat gtccccgtta ggcttagaga
    10861 cgtaccgctt cggccgcccc aggacataag cctcagcatc ctcataccga gtgaaggact
    10921 gcccaggaat tgtttggatt ccagcactct gcaggatctt ctgccccttc ttcctgtcca
    10981 gttcccaggt cgccccttcc agattgctcc caaacaccgg atagccctgg cgaatcagcc
    11041 tctccagatc ttccatcgca taggaattat ccagcatgac gatcatatcc gcccattttg
    11101 catggggctg ccaggcttca accttgggca ccagccccag accagctttg cacgatttac
    11161 tggcagggga aatgaacagc ttaacctgat ggtgagcctc ttggaacttc agcgccattc
    11221 ccaatccgca ggaatcttta tcgatgataa gaattttcat agtcagtctt tcagatatcc
    11281 gcgttggagt tgacgccggc gggcttcgga ggcttcgcgg gctttgtaag cctcagtctt
    11341 ggctttctgc tctggagttt tggacttgat gccgatcgcc ccaagggcaa actgtttgcc
    11401 ggacttatga cccgaggaaa tgtccatcgc atctcgagca ggggagatca tactccccac
    11461 tgcccacaag cccacatcgt ttgcagcccc cagcgcatta tcccccggtt cccaaatatg
    11521 cttgcctgta aacatttgcc tgttagcgtc cagttccgca atcatcttgg tagcaggtgg
    11581 aaggctccag gcttccttca acaggtcgtt aaccttcttg tcacccagga tgatgtgata
    11641 gaaaacattg gggattgtcg tagctccggc tctccgcatc tcagcatcgg gatcaccgct
    11701 gagatattta gcaatggaat ccagcatata aggatagacc acgaaaagtt tgaaggctga
    11761 gaacgcaaga tgcgcttgtg cctctccgcg agtctcaata ctctttgcgt cctgaacgag
    11821 gttatctcgc atcagatgcc caaaggactt catctgccca tagtggtaac gaccgaacag
    11881 atttccaatt ctcgattgca tggcccggga agcgaaacgg gaaatcgatt ccgccacagc
    11941 ctcaggcatg ttagggattt tcatcagctc gtcaaagccg atattcgagg gaacgcgata
    12001 ggtagggttg tgggcctcga cgtgcctgcg aattgcttgg ttcagaatgt ccgtcccctt
    12061 ctttgacgcc agatgtttgt acgctgtaat catcataaca tcggaaccac cccagagaat
    12121 cctgcgagac ttgtcgaaga tgtctgcaat catcctatcc ggccgtgcgc cgtagccgta
    12181 ctcctcggcc atagctttcc agccccgctc aggaatcccc ttaatcaggt tagcgaccgt
    12241 atctctagtt acgacagccg cgtactgcaa agccattccg gagttcaggt acttctgata
    12301 atccggggaa agcgtcgata catccttgta ggcttggtag aattccttac caagggtttg
    12361 gagttgccta ggatcgaacc atccccaacc tacagaatcg atcaagtgat cggccgcatt
    12421 gaacaagtga gggtaaggat tccagaacat cgtccccact gccgcagagt tgatgcgttc
    12481 aaggaggttt gtcgaggatc ccgatttaag caccccatcc tgaaaggtat tggcaagacg
    12541 gttctggata tagtattgtt ccagtgcccg atcccccaaa actctacgga agcctttggg
    12601 aatgcctgca gccgaatccg cctttattgc atagccttga tctgtcaagg ccttgagagt
    12661 ctcccccatc catttgcgtt cgcgaatgta ggactcgagc tcagcaaccg ccgcgagctt
    12721 gttagccagg gcgttcttgc tgtattccag tttcgtctga ctttcaatca tccgagttga
    12781 gacctggaca agtttcgcag ttcccccggc caccgtgaca ttttccccga ccttcagttc
    12841 cttacctgac cgggcaatga ctccaatggg attgcgatca gggccatatc cgatgagatc
    12901 acgcccctgc tgattcacca cgcgcaggct tccgtccggc atttgcaggc caaacatgct
    12961 gcgacccttc atgctgccgg cttgacggac aaacccgctt ccagggaaag caccttcccc
    13021 gaaggacaca accttgtcca tccagcggcg tttcgtcatc ctcggattgt gggggtcgag
    13081 atcggctatt tctttcccct cattcaccct gacagcttcc cggtagagcg cctcaagtct
    13141 cctggtcagt gggtccagga tggaggcctt cacagcctgt tcctcgggcg tcagccgatt
    13201 cgcagcacca ggaacctccc cttcctgcgc atcgtacatc cgctcgctag aggcgatctt
    13261 tcggacctct ggagagagtt gctcgaagta ggttttcgtc ctgatcatcg tggcttctcg
    13321 ctgtttcagg agttggtact gtccatccga gagagattcg gcttgctcgg tgacagtctt
    13381 ccccccaacc ttcgggtcag tggtgatggc cttctgcaac tgatcccaat ggaagcgagc
    13441 tgcttgttcg tattgggggc cgagtctgtc cacagcatcc ttgacgtagg cttccttcgt
    13501 tccgaggctg gggggaagct tctcgaccag gggcttgagc aggcgcagac caagagcagc
    13561 ccccgcggcg cccgtcaaga ggccgaagaa cttgttcctg tcatctgcca gataagcccc
    13621 gacaagcgcc ccaccagcaa ctgtccccat cgtcgcagca aggcgagggt caatctgccc
    13681 atacatgtta gtacgagggc cgcctgtttg ctccctggcc ttgtcgatgg agacttcaag
    13741 ccaaccgtgt ccatgctggt cagtgtaggg ctttgcgccg agggtcttga ggtaggaggc
    13801 tatatcacgg ttgtagcgat tatagatgga ttggtgttcg gggcggaaag gaggggaatt
    13861 ttggtattca gcaatcgttt cccgaagctc ttgtaacttt tcctgattta ttcgaattct
    13921 ttcgtccccg tatatctcgg gacgattctt tattttttgt tcccattcca attctactgc
    13981 aagctcttct tgcgcccgat ttagtctacg acgcccctca gattctggcc acccctccac
    14041 cttcgccaca gtgtctgcat c
//
LOCUS       DAOQLC010000110        13832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1630_length_13832_cov_21.069101, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000110 DAOQLC010000000
VERSION     DAOQLC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1630_length_13832_cov_21.069101"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PL000_16445"
     CDS             complement(<1..360)
                     /locus_tag="PL000_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90523.1"
                     /translation="MKQIEEYYAPWVKGIPMYISEHIELAWRKPELHRMMSNENPLPP
                     SDKVLEAMFKYAKMTNRYPDQGLIVRSKIAEINGVAGPQNVMIGNGSSEVYDNIFRMF
                     IEPGDEVIQHTPCFGIYG"
     gene            complement(448..1323)
                     /locus_tag="PL000_16450"
     CDS             complement(448..1323)
                     /locus_tag="PL000_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNC90524.1"
                     /translation="MSIVNAKEIMIPAAKEGWAVGAFNVTDLLQFEAVIDAAIEKKAP
                     VIVQTSVKPSQFLGTNMMVAIYRTLAESAPVPVCLHLDHSTSIDYCKKCADAGYTNIM
                     IDASKQSYEENIRQTKEVVDYCHSVGNISVEGELGTVGGVEDQIKVAEDEAQLANPEQ
                     SIEFVERTGVDIFAPAIGTAHGVYKTKNPKIDFERMATIHKMLNSNGIKTPLVVHGGT
                     GLPDDYVAKLLAAGGAKFNVSTELKHTLINAKWEYLNAHRDEYDPGKLDVFVRDATRK
                     AVMHWMDKLGCNGKA"
     gene            complement(1417..2352)
                     /locus_tag="PL000_16455"
     CDS             complement(1417..2352)
                     /locus_tag="PL000_16455"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HNC90525.1"
                     /translation="MTMTLAIDKAIAKVPFLAGSKNIKKTPLSGGITNLNFRIDADGR
                     SYVIRLAGEGTDQLGIKRDVEYAANKAAGELGIAPEVVYFIEPEGYIVTRFSNGKRIM
                     PEDIVKPDYLARVAKKIRLFHRRGPKLKGEFNVFKRVEMLTKVSKANNARFPHDWDFI
                     MQKMNEAKKALEKDPYVPTPCHNDLLNLNWLDEDVPGDIGEIRLLDWEYAGMGDIFFD
                     LGNFSHHHRLNEDQVDIFLYEYFGEITDKTYARLRLMWPMSELHEAMWGTTQTGISKL
                     EEDFQGYADLWFGRFRQHVTDFRWEQWLKDVAKKK"
     gene            complement(2464..4896)
                     /locus_tag="PL000_16460"
     CDS             complement(2464..4896)
                     /locus_tag="PL000_16460"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC90526.1"
                     /translation="MSEFPTQAQVVIIGGGVGGSSIAYHLTKLGWKDVVIIEKHELTS
                     GSTWHSAGLVGQMRSDANLTRMMHYSTDLYRSLKAETGQDTSWREVGGIRLASSAERM
                     EENRRLVGMARSFGVPMELISPKEAQDLFPLMDITGVVGAAYTPNDGSIDPTGLTNAL
                     AIGAKNRGARIFTNTTVTAINLKNGRVSEVVTDKGTIKTEVVVNASGMWGREIGKMVG
                     LSLPVLPMAHLYIMTKPIEGVTSKFPNLRDPDLLVYWREEVGGLITGGYERQPATFGM
                     NGIPKDFKFQLLPPDWDRFTPLMENSIRRVPAVANAEVKLLLNGPEGFTPDGEFLLGP
                     TTVKGFWVACAFCAHGLAGAGGIGKVMAEWIVDGSPEWDMWRLDVRRFGPNYNNLDYV
                     AARTFETYTQYYDIHYPGEERISRRNVRTSPTYFRLRDLGCSFGEKMGWERPNWYRIY
                     EEKATHGHEPKGWMRHNWSRAIGHEHLMTRANAGLFDETSFNKFEVRGPGALKFLNYV
                     CANQIDVPVGNLVYTQALNKRGGIECDFTVTRLAEDRFFIVTGTAFGQHDMSWLSLQM
                     PEDGSVTIEDVGSNYACIGMWGPKARTILEKVTTEDVSNAGFPYMTFKRMNVGDVPVL
                     ASRVTYVGELGWEFYCPMEYGLRLWDTLWEAGQSEGMVAGGYKAIDTLRLEKGYRYWS
                     GEISPDYTPYEAGLGFAVKLDKENFIGKDALVKQKAEGIKRKLCTIILDDDRTIVIGK
                     EPIRAGDKLVSWVASGGFGYSINKSIAYAYLPMEFAKAGTKLSVECFGEQVSAEVAQA
                     VLWDPKGERIRQ"
     gene            complement(4982..5791)
                     /locus_tag="PL000_16465"
     CDS             complement(4982..5791)
                     /locus_tag="PL000_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC90527.1"
                     /translation="MAKLVKDLMHPGIITCKPTATLGQVAVLLNQHNVHALFVIDRDG
                     RIMGVISDFDLMAGEWLSSDPDSLNVMRKLTAADLMSNPVDTISANTPLPEAVDQFMK
                     KGVSRFLVTENEKPVGILSLSDFVASIADNAKSKRDTVADVMSDAILVCRGKTPVASA
                     ARAMTSAGWRSVLVVDAKGKILGVVSGHDLLRFVKDGLGENLTVRDVMHPALTIDIHA
                     SLRDAANMMIQNHHHRLVVIDKDDPDAFPLGAISTFDIVAEMANPSSVWQK"
     gene            5935..6723
                     /locus_tag="PL000_16470"
     CDS             5935..6723
                     /locus_tag="PL000_16470"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HNC90528.1"
                     /translation="MGKPLIPAQRRERIQEYLAIHQIARTADLMNLLETSEATVRRDL
                     EWLEQKGILERTHGGAILNQRVIFEQEYQQRAQHFPEEKKMIGELAASLIEEGDIVFI
                     NSGTTATQVLQHIRRDTRVTIFTNNVNAALELGDAGFHYYLTGGEFQSRSNSLAGRFA
                     LDNLNMVFANKVILGVDGVSIKHGCTVPTNQEAEVVRKMIERTKGQVIVVADHSKWGA
                     VSNFPVANMGEVDKLVTDDGFGKEAIKDLAEHSVEVLLARNGKV"
     gene            6789..9218
                     /locus_tag="PL000_16475"
     CDS             6789..9218
                     /locus_tag="PL000_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC90529.1"
                     /translation="MKTQAEIVIIGGGIYGAQAAYHLAKNGRKDVVIIEKGEIASGES
                     SHAAGLVTQFATSQAMLKFRMYSVQMYKELGLFSTVGSLRVASSKEQLLEMERSVSRA
                     KALGLDCEVIGPEETKRAMPQISEKNLFGGIYLPGDGQLDPYTTTTSMAKFARDLGVE
                     IYTNTRVTGIKVSAKGEVQAVVTDKGEIRCEIIINAAGMWAPRIAAMAGLHVPTTPVD
                     HQHIALKAVPGHEFAPDTPCLRDPDNLVYMRQEQGGLVIGGYEPTPLPRWIDGTPWEH
                     GSKSFPGDFDQFEMLLEGAIRRLPFLDQAGIITLVRHPGAYTPDCQPLLGPMAGVKGF
                     WMMAGMSLNGYGGAGGMGKLMAEWIIDGEAPMDVYGYRATRFGNYYSDFKYAAERTVE
                     SVKYYYRLKFPHDEHEHGRPHRTSPVHYRLMENGAVFGEKFGWERVNYFDPGKEWRRM
                     GEDQREWGWAKPPFFERMRQEHIATRERVALFDLTSFGKIEVKGKGALPLLQRLTSSN
                     VDKPIGSAVYTQFLNSRGGIESDLTVTRLGEDYFWVITGSGFIANDLARIQMHVDEND
                     GEVSIRDITMEHACLALWGPKAREVLRKITSSDVSNESHPYLMTKPIAINGVKVLAQR
                     VSYAGELGWELYIPNNRATMVWDMLIEAGKEFGMELGGYKVLDPLRLEKGFKYFTTDI
                     TPTVTPYEAGLGFCVDLNKGDFIGKEALVAQKAQGVKRKLCTLVLTDTEDYVQIYGGE
                     AVHHEGKVITRVRSGGYGFTVKKNILYAFLPTELAVKGNRFVIELIEGNREAEVTSSV
                     LVDPKGERLRA"
     gene            9274..10353
                     /locus_tag="PL000_16480"
     CDS             9274..10353
                     /locus_tag="PL000_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNC90530.1"
                     /translation="MPSAKQTPPSYVGFGMLTPVYIMALDQLPKHNTGAIVHQVSEYV
                     YDDAAIIACNLRQWNVPTGMIGTSVGDDLLGHHIENTLKNMGVQGKVRFTKKYKTPLE
                     VNVSDKLGARTYFWQRSPEILSTLDTADLGMLKDADLMYVDWYDGDHIIRAMNEARRC
                     GVPVFLNLEHGHRDPEIMEKYVSRATICQAVTDDAQIGRKGAMLTVAKKLLKAGIKTA
                     IITMARQGCMVVQGDEIIRVYAPKVKAVDGSGAGATFSSGFIYGHLKGWGLEETVRFA
                     IAAASLKVTRSGLKMFPISEIKKLAASLSVERMEYRDNQFFELNDLISQAQKSPLVKE
                     SKKLAEKLMLKKKTERKKIKKSLVE"
     gene            10405..11841
                     /locus_tag="PL000_16485"
     CDS             10405..11841
                     /locus_tag="PL000_16485"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HNC90531.1"
                     /translation="MSFAAFLTQEQVERIHEASLEILEEVGLKVRYEPARAMFEKHGC
                     SVDGERVKFPREVVEKYRKMCPPTFTFYARDPKFDRTIPRDSPVIVTASSAPDIIDPV
                     TGHERRAESGDIARIAHLINELPGYDMFSISTLADDAPAGQFTISRLYPALKYCLKPI
                     RVTTTDHKDTLSVMKMAYMVAGGEEAYKEHPFLTHHYCPTVSPLSMDHLSTENVMYFA
                     SEGLPVYPTIVPNAGLTSPMSMAGTLVQGNAEFLATTVLMQMVKEGTPTIYATLATVA
                     DMRTGAYTSGAIECGMLHMAFAQMSRFYNVPCGGYVGLTNSKLNDAQSGYETGMSAMG
                     GLLGGMDMFNVGGLIDALKTFDFAKAVIDDEVAQMLKRAKRGVNFSEDELAIGLIKEI
                     GPGGSFITAKHTISRMKTEAVMTKLADRDARTTWEKKGSTDIHARAMKRVKEVMTSNT
                     TPLISPELDDKLRAEFPGMVAGMLHPIE"
     gene            11854..12474
                     /locus_tag="PL000_16490"
     CDS             11854..12474
                     /locus_tag="PL000_16490"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNC90532.1"
                     /translation="MFGFFKKKESVFQNLIEQQASLAYEGLRLLVKYFETNDPEVAEE
                     LSIKEKEADEVRRILIDELNRSFITPFDREDIFALSRSIDDVIDYADTTVMEMVVLKV
                     APTPFMLRIASLLKDAAYEIMHGVQRLPKHPKVAIDHAQRAKALENRVEAVYREAIAD
                     LFSGPEDIHHLVEMLKMREVYRHLSNAADRGDEAANIIADIVVKKT"
     gene            12545..13477
                     /locus_tag="PL000_16495"
     CDS             12545..13477
                     /locus_tag="PL000_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNC90533.1"
                     /translation="MRDSSNIVATMISSRAFHPQTALGVTAVAEFLGPFLFGVTVAKT
                     VGSDIVDSSLLSLDALVACLAAAIIWNLITWFLGIPSSSSHALIGGLIGVAAFTVGFG
                     SINVHAIYKVLTGLFVSPLAGFVVGFMILRMIYFLAQNASPNINELFKRAQLLTAIGL
                     AFSHGTNDAQKTMGIITLSLIIGGELTGFQVPFWVIAVSASTMALGTAMGGWRLIRTL
                     GGKFYKIRPVHSFATQITSAVVILAASFGGAPVSTTQVVSSAIVGVGSSERFGKVRWG
                     VVSDILTAWIITIPVSGLISAGIYWFVESWIRGI"
     gene            13640..>13832
                     /locus_tag="PL000_16500"
     CDS             13640..>13832
                     /locus_tag="PL000_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90534.1"
                     /translation="MRTKNYAYQLFALFVIAATLLAACGGGAPAATEAPAATEAPAMT
                     EAPAATEAPAATEAPADPMA"
BASE COUNT         3259 a         3298 c         3676 g         3599 t
ORIGIN      
        1 gccgtaaatg ccgaaacagg gggtgtgctg aatgacttcg tcgcccggtt cgatgaacat
       61 gcggaagatg ttgtcgtaca cttcgctgga accgttaccg atcattacgt tctgcggacc
      121 cgcaacaccg ttgatctcgg cgatcttgct gcggacaatg agaccctgat cggggtagcg
      181 gttggtcatc ttggcgtact tgaacatggc ctccaacacc ttgtcagaag ggggcagagg
      241 attttcgttg gacatcatgc ggtgcagttc gggcttgcgc catgccagtt caatgtgctc
      301 cgaaatatac atcgggatgc cctttaccca cggggcgtaa tattcttcaa tttgtttcat
      361 tactttgatc tcctgattta ttttgtttat ttcaggctgc aaggagctta actgtcaacc
      421 tgccaacctg taacttgcaa cctgttatta cgccttgccg ttgcagccga gcttgtccat
      481 ccagtgcatg actgccttgc gggtcgcgtc gcggacgaag acatcgagct tgccgggatc
      541 gtattcatcg cgatgggcat tcagatattc ccatttggcg ttgatgaggg tgtgcttcaa
      601 ctcggtggaa acgttgaatt ttgcgccgcc agccgcgagc aacttggcaa cataatcatc
      661 gggcagtccg gtgccgccgt ggaccacgag cggggtcttg atgccattgc tgttcaacat
      721 cttgtgaatg gttgccatgc gctcgaaatc gatcttcgga ttcttggtct tgtacacgcc
      781 gtgcgcggtg ccgatggcag gggcgaagat atccacaccg gtgcgttcaa cgaattcaat
      841 ggattgttcg gggttcgcga gctgcgcttc atcttccgca actttgatct ggtcttccac
      901 gccgcccacg gttcccaact caccttccac agagatattg ccgacactgt ggcagtaatc
      961 caccacttcc ttggtctggc ggatattctc ttcgtaggac tgcttggagg catcgatcat
     1021 gatgttggtg tagcctgcat cggcgcattt cttgcagtag tcaatgctgg tggaatggtc
     1081 aaggtgcaga cagacgggta caggcgcaga ctcggccagt gtgcggtaga tggccaccat
     1141 catgttggtg ccgaggaatt gtgagggttt tacagaggtt tgcacaatga ctggagcctt
     1201 cttttcaatg gctgcatcga tcaccgcttc aaattgcagc aagtcggtga cgttgaatgc
     1261 acctacagcc cacccttctt ttgccgccgg gatcataatt tcttttgcat ttacgataga
     1321 cataattttc tccttacaag gtatcctttt gttcgatttt tgaaactttg tgaaaaggat
     1381 gaatgttttt ttaccttcat cctttatctt tcaatcttat tttttcttgg cgacatcttt
     1441 caaccactgt tcccagcgga agtcggttac atgctggcgg aagcggccaa accataagtc
     1501 ggcataaccc tgaaaatcct cttccagttt ggagatgccc gtttgtgtgg tgccccacat
     1561 cgcttcatgc aattcagaca tgggccacat caggcgcagg cgggcatatg ttttatcagt
     1621 gatctcacca aagtattcat acaggaagat atccacctgg tcttcattca aacgatggtg
     1681 atggctgaag ttgcccagat caaagaaaat atcgcccatg cctgcgtatt cccagtccaa
     1741 caggcggatc tcgccaatat caccggggac atcttcatca agccagttca gattaagcag
     1801 gtcattgtga cagggcgtcg gcacatatgg gtccttctcc agtgcttttt tggcttcatt
     1861 cattttttgc atgatgaaat cccaatcatg cgggaatctt gcgttgtttg cttttgaaac
     1921 cttggtcaac atttcgacgc gtttgaacac attgaactcg cccttgagct ttggtccgcg
     1981 gcgatggaat aatcggatct tctttgcaac gcgtgccaga taatctggct tgacaatgtc
     2041 ctcaggcatg atgcgtttgc cgttgctgaa ccgcgttacg atataccctt caggttcgat
     2101 gaaatagacc acttcagggg caatcccaag ctcgccagcc gctttgtttg cggcgtactc
     2161 cacatctcgt ttaatgccga gttggtcagt gccttccccc gcaagacgga tcacgtagga
     2221 gcgaccatcc gcatcaatcc tgaagtttaa atttgtgatg ccaccgctca gcggggtttt
     2281 cttgatattt ttcgagcctg ctaaaaacgg tacttttgcg atggctttat cgattgctag
     2341 agtcattgtc atgaaatcct tcctttggtt ggttttggat tttttccaag gcatcgggag
     2401 tcttttggat tcccgatgcc ttgatgattc aacgcatcgt tcctaactcg tacctgatcc
     2461 tgtttactgg cgaattctct cgcccttcgg gtcccacaac acagcctgcg ccacctccgc
     2521 gctgacctgt tcaccgaaac attccacgct caattttgtg cctgcctttg caaactccat
     2581 cggtaaatac gcatacgcaa tggacttgtt gatcgaatac ccaaatccac ccgaagccac
     2641 ccagctcacc agcttatcgc cagcgcggat cggctccttg ccaatgacga tcgtgcggtc
     2701 atcatcgagg atgatcgtgc agagtttgcg tttgatccct tcagcctttt gcttgaccaa
     2761 tgcatccttg ccgatgaaat tctccttgtc aagtttcact gcaaagccga ggcccgcttc
     2821 gtagggggtg taatcgggcg aaatctcacc gctccaatat cgatagcctt tctcaaggcg
     2881 cagggtgtca atggccttgt atccacctgc caccatgcct tcagactggc ctgcttccca
     2941 gagtgtatcc cagaggcgca gaccgtattc catcgggcag tagaactccc atcccagctc
     3001 gcctacataa gttacgcgcg aagcgaggac agggacatcc cccacgttca ttcgtttgaa
     3061 ggtcatgtac gggaagccag catttgaaac atcctcggtg gtgacctttt caaggatggt
     3121 acgggccttc ggtccccaca taccgataca ggcatagttg gaacccacat cttcaatcgt
     3181 cacactgcca tcttctggca tttgcagtga cagccaggac atatcgtgtt gaccaaatgc
     3241 ggtacctgtc acaatgaaga agcggtcttc cgcaagtctg gtgacggtga aatcgcactc
     3301 aatgccgccg cgtttgttga gcgcctgtgt gtacacaagg ttcccgacag gtacgtcgat
     3361 ttggttcgcg caaacataat tgaggaattt cagcgcgcca ggtccgcgta cttcgaattt
     3421 attgaaggaa gtctcatcga acagacctgc atttgcgcgg gtcatcaaat gctcatgacc
     3481 gatggcgcgt gaccaattgt gacgcatcca gcctttgggc tcgtgaccat gggtggcttt
     3541 ttcttcataa attcgatacc aattcggacg ttcccagccc atcttctcgc caaaagaaca
     3601 gccaaggtcg cgcaggcgga agtaagtggg ggaggttcgc acattgcggc gagagattct
     3661 ttcctctccc gggtagtgga tatcgtaata ctgggtgtac gtttcaaatg tgcgggctgc
     3721 tacataatca agattgttgt agttcgggcc aaagcggcgc acgtcgaggc gccacatgtc
     3781 ccattcgggg ctgccatcta cgatccattc ggccatgacc tttccgatac cgcccgctcc
     3841 agcaagacca tgcgcacaga atgcacacgc cacccaaaaa cccttgaccg ttgttgggcc
     3901 aagcaggaat tcgccatcgg gggtgaagcc ttcggggcca ttcaacagca atttcacttc
     3961 tgcgtttgcc accgcaggca cacggcggat tgaattttcc atcaacgggg tgaagcggtc
     4021 ccaatctggc ggaagcaatt ggaatttgaa atctttcgga attccattca tgccgaatgt
     4081 ggcaggttgg cgttcgtaac cgcctgtgat caacccgcca acttcttcac gccaatacac
     4141 cagcaggtca ggatcacgca ggttcgggaa tttactggtc acaccttcga tgggcttggt
     4201 catgatgtaa agatgtgcca ttggaaggac tggcagactc aacccgacca ttttgccgat
     4261 ttcgcgtccc cacatccccg aggcattgac gacgacttcg gttttgatcg tgcccttgtc
     4321 ggtaacgact tcacttacac gcccgttctt gagattgatg gcagtaaccg tggtgttggt
     4381 gaaaattctt gcaccgcgat tctttgcgcc aatggcgagg gcattggtca acccggttgg
     4441 gtcaatgctg ccatcgttgg gggtgtacgc tgcaccgacg acgcctgtaa tgtccatcaa
     4501 tggaaacagg tcttgtgctt ccttggggga gatcaattcc atcggtaccc cgaaggaacg
     4561 ggccatgccc accagccgcc tgttttcttc catgcgttca gcagaagaag caaggcggat
     4621 gccgccgact tctcgccatg aggtgtcttg tcctgtttcg gctttaaggc tgcgatacaa
     4681 atctgtgctg tagtgcatca tgcgggtgag gttggcatct gaacgcattt gaccaacgag
     4741 tcccgccgag tgccatgtgg agcctgatgt cagttcgtgc ttttcaataa tgacaacatc
     4801 cttccaccct agctttgtga ggtggtaggc aatacttgaa ccgcccacac ctccgccgat
     4861 gataacgacc tgtgcctgtg ttggaaattc ggacatgctg tgctcctgta tatggaataa
     4921 tcaatataac caaagacaga ggacgaaggg taacagcatg gtcatccttc gtcctctgtt
     4981 gttatttctg ccaaaccgag ctgggatttg ccatttctgc gacaatgtcg aatgtggaaa
     5041 tcgcaccaag ggggaatgcg tctgggtcgt ctttatcaat tacaaccagg cggtggtgat
     5101 gattttgaat catcatgttt gcagcatcac gcaggctggc gtggatatca atggtcagtg
     5161 cagggtgcat tacatcgcgg acggtcagat tctcgccgag gccatctttg acaaaacgca
     5221 acaggtcatg tccgctgaca acgccgagga tcttgccctt tgcatccacc accagaacag
     5281 accgccatcc agcggaggtc atggcgcggg cggcagaagc cactggtgtt ttgccgcggc
     5341 aaaccaaaat ggcatctgac atgacatcag caaccgtgtc acgttttgat ttggcattgt
     5401 cggcaatgct tgcgacaaaa tctgacaggg agagtatgcc cacaggtttt tcgttctcag
     5461 ttaccaggaa gcgtgaaacg ccttttttca taaactggtc cactgcttcg ggtagggggg
     5521 tgtttgcaga gatggtatcc accgggttcg acataagatc cgcagcggtt agttttcgca
     5581 tcacattcaa gctgtcgggg tcggaggaaa gccattcgcc tgccatcaaa tcaaaatctg
     5641 aaatgacccc cataatgcgc ccatcacggt caatgacaaa aagggcatgc acattgtgtt
     5701 gattcaaaag tacggccacc tgtcctaatg tggctgttgg cttgcaggtg atgatgccgg
     5761 ggtgcatgag gtctttgaca agttttgcca tggcagctcc aataaaatca aaaccaagac
     5821 ctgacaggtt tgacggtgat tattgagcct gtcaggtctg agcggaattg aacgaattat
     5881 aatgaaaaaa tcaaaaactt gcaaattcct tcaaattctt atataatccg ccagatggga
     5941 aaacccctta tcccagccca acgaagagag agaattcagg agtatcttgc aattcatcaa
     6001 atcgcgcgca ctgccgactt aatgaatttg cttgaaactt ctgaggcaac cgtccgccgc
     6061 gatcttgagt ggctcgaaca aaaggggatc cttgagcgta cgcatggggg cgcgatcctg
     6121 aatcaacgcg tgatctttga gcaggaatat caacagcgcg cgcagcattt tccagaagag
     6181 aaaaaaatga tcggcgaact tgccgcgtct ttgatcgaag aaggcgatat tgtttttatt
     6241 aacagcggaa cgactgccac acaggttctt caacatatcc gccgtgatac gcgggtgacc
     6301 atttttacta ataacgtaaa tgccgctttg gaattgggtg atgcgggttt tcactactat
     6361 ttgacaggcg gtgaattcca gtcacgctcc aactcactgg caggccgctt cgcgctggac
     6421 aatttgaaca tggtctttgc caacaaggtg attctcggcg ttgacggagt ttccataaag
     6481 catggttgca cggttcccac caatcaggaa gccgaggttg tgcggaagat gatcgagcgt
     6541 accaagggcc aggtgatcgt ggtggcagac catagcaagt ggggcgcggt gtcaaacttc
     6601 cctgtggcaa acatgggtga ggttgacaaa cttgtaactg acgatggttt cggcaaggaa
     6661 gcgatcaaag accttgctga acattctgtg gaagtattgc tcgccagaaa tggtaaagtg
     6721 taaagacgaa aactgatgaa caggcgcatg atgggcctgt tcatttatct actctcaatg
     6781 gagcgaatat gaaaactcag gcagagatcg ttatcatcgg tggtggaatt tatggagcgc
     6841 aagccgccta tcatcttgca aagaatggcc gtaaggatgt ggtcatcatt gagaaaggcg
     6901 aaattgccag cggtgaatct tcacatgcgg cgggtttggt gacgcaattt gcgacctcgc
     6961 aggccatgct caaattccgc atgtacagcg tgcagatgta taaagagctg ggattgttca
     7021 gtaccgtagg aagtctgcgg gtagcctcga gcaaggaaca gttgctcgaa atggaacgca
     7081 gtgtcagccg cgccaaagca ttgggtttgg attgcgaagt cattggccct gaagaaacca
     7141 aacgcgccat gcctcaaatc tcagaaaaga atttgttcgg cggcatttac ctgcccggtg
     7201 acgggcagtt ggacccatat accaccacca cgtccatggc gaagtttgcc agggatctcg
     7261 gtgtggaaat ttacaccaac acccgcgtga ccggcatcaa agtttcagcg aaaggggagg
     7321 ttcaggcggt tgtaacagac aaaggtgaga ttcgatgcga gatcatcatc aacgcggcgg
     7381 ggatgtgggc gccgcgtatt gctgccatgg cggggttaca cgtcccaacc acccctgtgg
     7441 accatcaaca cattgccttg aaagctgtgc ccggacatga atttgcgccc gatactccct
     7501 gcttgcgtga ccctgataat ctggtttaca tgcgtcagga gcagggcggt ttggtcattg
     7561 gcggttacga gccaaccccg cttccacgtt ggattgatgg gacaccctgg gaacatggca
     7621 gcaagagttt cccaggtgat tttgatcaat ttgaaatgct gctcgaaggt gccattcgtc
     7681 gccttccgtt tttggatcag gcgggtatta tcacactggt tcgccatccc ggcgcataca
     7741 cccctgattg tcaaccctta cttggaccga tggctggtgt caaagggttc tggatgatgg
     7801 cggggatgtc gctcaatggt tatggcggcg caggcggaat gggtaagttg atggccgaat
     7861 ggatcattga cggcgaagcg ccaatggatg tgtatggata tcgtgccacc cgctttggaa
     7921 attattattc tgactttaag tatgcagcgg aacgcactgt tgaaagtgtg aagtattact
     7981 atcgtttgaa attcccgcac gacgagcacg aacatggtcg cccgcatcgg accagcccag
     8041 tgcattatcg tttgatggaa aatggcgcgg tctttggcga aaaattcggc tgggaacgtg
     8101 taaattattt tgatcctggc aaggaatggc gccgaatggg cgaggaccag cgtgaatggg
     8161 ggtgggcaaa gcctccgttc tttgaacgta tgcggcagga acatattgcc acacgtgagc
     8221 gcgtggcctt gtttgacttg acatcgttcg gcaagatcga agtcaaaggt aaaggtgcgc
     8281 tgccgttgtt gcaaagactg acttcaagca atgttgataa gcccataggc agtgcggtat
     8341 acacccagtt ccttaattca cgcggcggca ttgagtctga cctaacggtt acccgtttgg
     8401 gagaagatta tttctgggtc atcacgggtt cgggttttat tgccaacgac cttgctcgta
     8461 ttcaaatgca tgtggatgaa aatgacggag aggtgtccat ccgtgatatt acgatggaac
     8521 atgcctgcct tgctttgtgg ggaccgaagg cacgtgaagt gctgcggaaa attacaagca
     8581 gtgatgtttc gaacgagtcc catccgtacc tgatgaccaa acccatcgcg atcaacggtg
     8641 tgaaagtgtt ggcgcagcgt gtcagttatg cgggtgagtt gggctgggaa ctgtatattc
     8701 caaacaatcg cgccaccatg gtttgggata tgctgatcga ggcagggaag gaatttggta
     8761 tggaacttgg cggttataaa gtgctcgatc cattgcgcct ggagaagggt ttcaagtact
     8821 ttaccaccga catcacaccc acggttaccc cttatgaagc agggcttggt ttttgtgttg
     8881 atttgaacaa aggtgatttt atcggcaaag aggcattggt tgcgcaaaaa gcacaaggtg
     8941 tgaagcgtaa actttgcaca ctggtattga ccgacaccga ggattatgtt cagatttatg
     9001 gtggtgaggc ggttcatcat gaaggcaagg tcatcacccg tgtgagaagt ggcggatacg
     9061 gctttaccgt caagaagaat attttgtatg ctttcctgcc cacagagttg gcagtgaaag
     9121 gcaaccgttt tgtcattgaa ctcattgaag gcaaccgcga ggcagaagtg acctcatctg
     9181 tattggttga cccgaaaggc gagcgtttac gtgcctgata ttgtgtaatc gcaaagagtt
     9241 tgcaggcctt tgcgaaattt tgtggtgaaa agaatgcctt ccgctaaaca aactcctcca
     9301 tcttatgtag gctttggaat gttgacgcct gtgtacatca tggcactgga tcaactaccc
     9361 aagcacaaca ctggcgcgat cgtacatcag gtcagcgaat atgtgtatga cgatgctgcg
     9421 atcattgcct gcaatttgcg ccaatggaat gttccaacgg ggatgatcgg tacatccgtg
     9481 ggtgatgatt tgctgggtca tcatattgag aacaccctga aaaatatggg ggtgcagggc
     9541 aaggtgcgtt tcaccaaaaa atataaaaca ccccttgaag tgaatgtttc agataaattg
     9601 ggtgcaagga catacttctg gcagcgttct ccagagattc tttctacctt ggacactgcg
     9661 gatttgggaa tgttgaaaga cgcagatctg atgtatgtag attggtatga cggtgaccat
     9721 atcatccgcg caatgaatga agcacggcgc tgtggtgtgc ctgtctttct taatcttgag
     9781 catggtcatc gtgaccctga gatcatggag aagtacgtaa gccgcgccac catttgccag
     9841 gcagtgaccg atgatgccca gatcggcaga aaaggcgcaa tgttgaccgt tgccaaaaaa
     9901 ctgctcaaag cgggtatcaa aaccgcgatc atcaccatgg caaggcaggg gtgtatggtt
     9961 gtgcagggtg atgagatcat ccgtgtgtat gctcccaagg tcaaggcggt ggatggttcg
    10021 ggggcgggcg cgaccttttc gtcgggcttc atctatgggc atctcaaagg atgggggctt
    10081 gaagagaccg tccgctttgc aattgcggca gcttcactca aggtgacccg ctctgggttg
    10141 aagatgtttc cgatcagtga gatcaaaaaa ctggcggcgt ccctttctgt ggagcggatg
    10201 gaatatcggg ataatcagtt ttttgaattg aacgatctga tctcgcaggc acagaaaagt
    10261 ccgctggtta aggaaagcaa gaagttggct gaaaaactga tgttgaaaaa gaagacggaa
    10321 cgaaaaaaga tcaagaagtc gctggtggag tgattcacca tgaaacgaat attgaaaccc
    10381 ttatcaattt aaaggatgtg aaccatgtca tttgctgcat ttcttaccca ggaacaggtt
    10441 gaacgaattc acgaggcttc acttgagatt ttggaggaag ttggtctgaa ggtgcgttat
    10501 gaacccgcac gggccatgtt cgaaaaacat ggctgctcgg tggatggcga acgggtgaaa
    10561 tttccccgtg aggtggttga aaaataccgc aagatgtgcc cgccaacctt cactttctac
    10621 gcacgtgacc cgaagtttga ccgcacgatt ccccgggata gcccggtcat tgtcacggcg
    10681 agttccgctc cggatatcat tgaccctgtt acgggtcatg aaagacgcgc tgaatctggc
    10741 gatatcgccc gaatcgcaca tctgatcaat gaactgccag gctacgatat gttttccatt
    10801 tccacactgg cagatgacgc accggcgggt caattcacaa tctcgaggtt gtatcctgct
    10861 ctcaaatatt gtcttaaacc gatccgtgtc accacgaccg atcacaaaga taccttgagc
    10921 gttatgaaga tggcgtatat ggtggcgggc ggtgaggagg catataagga gcatccgttc
    10981 ctgacgcacc attattgccc gacggtttca ccgctttcaa tggatcatct ttccactgag
    11041 aacgtaatgt attttgcaag tgaaggcctg cctgtttacc cgaccattgt gccgaatgcg
    11101 gggcttactt cgcccatgtc aatggctggg acgctggtgc agggtaatgc ggaattcctg
    11161 gcgacaactg tgttgatgca gatggtgaag gagggtactc caaccatcta tgccactctt
    11221 gcaacagtag ccgatatgcg cacaggcgcc tatacttccg gtgccattga gtgtggaatg
    11281 ttgcacatgg cttttgcgca aatgtcccgt ttttataatg tgccttgtgg tggttatgtc
    11341 ggcctgacga attccaaatt gaacgatgct caatctggct atgagacggg catgtcagcc
    11401 atgggcggcc ttttgggcgg catggatatg ttcaatgtcg gcgggctgat cgatgcgttg
    11461 aagacattcg attttgcaaa agccgttatt gacgacgaag tggcacaaat gttgaagcgt
    11521 gccaaacgcg gcgtgaattt ttcggaggat gaacttgcga tcggcttgat caaagagatc
    11581 ggccctggcg ggtcgtttat taccgcgaaa cataccatca gccgtatgaa gacagaagcc
    11641 gtgatgacca agttggccga ccgtgatgcg cgtacaacct gggaaaagaa aggttccact
    11701 gatatccatg ctcgtgcaat gaaacgcgta aaggaagtca tgaccagcaa tacaaccccg
    11761 ctgatttccc ccgaattgga tgataagctc cgcgcagaat tcccgggaat ggtggcagga
    11821 atgctccacc caattgagta gtgaaaggta tttatgtttg gctttttcaa gaaaaaagaa
    11881 agcgtttttc aaaatttgat cgaacagcag gcctctctgg cttatgaagg gttgcgcctg
    11941 ttggtgaaat attttgaaac caatgatcca gaagtggcag aggagctctc gattaaggaa
    12001 aaagaagcgg atgaagtgcg ccgcatcctg atcgatgagt tgaatcgcag ttttatcact
    12061 ccgtttgacc gcgaagatat ttttgcgctt tcccgctcca ttgatgatgt cattgattac
    12121 gccgatacga ccgtaatgga aatggttgtg ttaaaggtcg ccccaacccc ttttatgctc
    12181 cggatcgcat ctttgttgaa agatgctgct tatgaaatta tgcatggggt tcaacgtttg
    12241 ccaaaacatc caaaggtggc aatcgatcat gctcaacgcg cgaaggcgtt ggagaatcgt
    12301 gtagaagcgg tttatcgtga agccattgca gatctgttca gcggccctga ggatattcac
    12361 cacctggtcg aaatgttgaa gatgcgcgag gtttatcgtc atctctccaa cgcggcggac
    12421 cggggggatg aggcggccaa tatcattgcc gatatcgttg taaagaaaac ttaacaaggt
    12481 gttaatgtct tctgaattgc tccttgttat cattctggcg ctggtttttg aattcatcaa
    12541 tggtatgcga gattctagca atattgtagc gactatgatt tcctcgcgcg cattccatcc
    12601 gcaaaccgct ctgggcgtaa cggcagtggc agaattttta ggcccgtttt tgtttggcgt
    12661 gaccgtggca aaaacagtcg gcagtgatat cgttgattcc agcctgttgt cgttggatgc
    12721 actggttgcc tgtttggcgg ctgccattat ttggaacctg attacatggt ttcttggaat
    12781 tcccagcagt tcatcgcacg ctttgatcgg cggtttgatc ggcgtagctg cctttacggt
    12841 aggctttggt tcgatcaatg tccatgcgat ttataaggtt ttgaccgggt tgttcgtttc
    12901 gccgttggct ggttttgttg tcggttttat gatattaagg atgatctatt ttcttgcgca
    12961 aaatgcatcc ccaaatatca atgagttgtt taagcgtgct cagttgctca ctgccatcgg
    13021 tctggctttc agtcatggaa cgaatgatgc ccaaaagacg atgggtatta tcacgctgag
    13081 tctgatcatc ggcggtgaat tgaccgggtt tcaagtcccg ttttgggtta ttgctgtcag
    13141 tgcatccacc atggcgttgg gtactgccat gggcgggtgg aggctgatcc gcacgctagg
    13201 cggaaagttt tataagatcc gcccggtaca tagttttgcc actcagatca cttcagcggt
    13261 cgtaattttg gctgcatcat ttggtggagc cccggtcagc accactcagg ttgtaagttc
    13321 tgccattgtt ggtgttggtt catcggagcg gtttggaaag gtacgttggg gtgtggttag
    13381 tgatatcctt actgcgtgga tcattacgat ccctgtcagt gggttgattt cagcaggtat
    13441 ttattggttt gttgagagtt ggattagagg tatttaacca ccattcttgt tcatttattc
    13501 aaagggccgt ttgactttga atattgataa tgctaaaatg ttaacgctgt gttaacggat
    13561 cggaggtgtc tgggaagaga agtctgtcat gtgtttccat tcgtctgatc aaccaaaacc
    13621 caaaaggaga tagagagaaa tgcgtacgaa aaattatgct tatcaactgt tcgccttgtt
    13681 cgtaattgcg gccaccttgt tggctgcttg tggtggtggt gcccccgccg ccactgaagc
    13741 ccctgctgcc actgaggccc ctgccatgac cgaagcccct gccgccaccg aggctcctgc
    13801 tgctaccgaa gctcccgccg accccatggc tg
//
LOCUS       DAOQLC010000111        13826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1632_length_13826_cov_25.319657, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000111 DAOQLC010000000
VERSION     DAOQLC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_13826_cov_25.319657"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..3712
                     /locus_tag="PL000_16505"
     CDS             83..3712
                     /locus_tag="PL000_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HNC90535.1"
                     /translation="MTVDKPTLNKTENTVVFTITVSNGGPDNATGVKVKDLLPAGLTY
                     SSHSPGETYNSGTGIWDVGTLTSGGVGSTDSLTITATVTASFLPITNKAEVSSWDAGQ
                     TDPNSTPGNNSTTEDDDASTIITTQNANLSLTKTVNTTTPIVGSNVVFTVNVQNTGPF
                     QATNVTVTDLLPGSLTYISHTGAGTYVPGTGLWTLPTPLGVGAGASLQITASVDVPGP
                     ITNTATISNSDQYDNNPANDQASITLNPDTSDLSLSIAVNNTTPDKNDNVVFTVTLNN
                     AGPTTATNVVVTDVFPSGLSNIIDDDGGVGAPYPYNSNTWTIPSLPSGASAVLNITAN
                     VITQGAKIYSAQVWSSDQLDPNSTPGNNSTTEDDDASISLIPEVSDLSLIKTVDNPIP
                     NNGDTVVFTITVNNNGPDIATNVLVADVLPVPFTYISDDDGGGAFPYNAGVWSIPSLA
                     NGASAILHITALTGPGTLNTNYAEIMTSDQIDPDSTPGNGLIAEDDQDGSPSADLSLS
                     MTVSNPSPPLGSDITFVLTVNNIGVGTANNVFVKDILPAGLEWQSDNGAGAYNSGTGL
                     WNVGSLTPSGVGSTATISIVAKVMSTGVKTNFAEIVTSSLLDPDSTVSNGSVTEDDDA
                     NAVVTPAAGMLVIISEVAWAGTVAEGDDEWIELYNPGTSDININGWKLKDNSSIISLT
                     GSIPAGGYYLIEDTSVAVSDVTHDLIFNMSLSNSGESLELYDGSNILIDTANKDGGSW
                     PAGDVDTKGTMERIVSKTDSDDAWVTNTGFIKNGKDRNGGDIWGTPKNPAPVSTPTPT
                     PSGGMSIVISEVAWAGTAANSSDEWVELYNPGASQIDITGWKLIDGGGTEIILNGKID
                     PGSFFLLEDNENTVSDIIASQLDGNLALTNEGETLQLLDGSNTIIDSANNDGGGWPAG
                     SNSTYGTMERIVSSPDGNNAWYTNAGIVKNGKDANGGDIWGTPKQGNSPIPTATATAT
                     PITQTPTRTLTPTKTVTPTRTVTRTPTAEPVGRPVINEFLARPGYDWNRDGFVDVYDE
                     FIEIKNIGIVEINLKGWKLDDEADSGSTPFTFPDLLLLPGDRVVFYGNETRILLSDGG
                     DTVRLLNASNKVYDAYTYPLAKFKDKSVCRMPDGNGSWYEDCIPTPNLTNSRQGSVPS
                     MPGGSNAESELCDFPDTMPEDFVYAECRGFGGNIWDTFFWDQFGWLGRMIFQPASSKW
                     EIIVE"
     gene            3808..4644
                     /gene="pyrF"
                     /locus_tag="PL000_16510"
     CDS             3808..4644
                     /gene="pyrF"
                     /locus_tag="PL000_16510"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNC90536.1"
                     /translation="METFFTFLEKRVDDSSSLLCIGLDPHIPDLKEPTAESALDFCLT
                     LIKQTAPYAAAFKPNAAFFEVFGAEGWLALKQVIEAINAESNRLGSRIPIILDAKRGD
                     IASTADAYARSAFENLGADCITLNPYLGKDSIEPFIQNSEKGVFLLCKTSNAGSMDLQ
                     NLLVLPMGSDTPMPLYIYVAKLAQEWNEKNNIGLVVGATHPQIMEMIRAAVPNLWFLS
                     PGVGAQGGELQLALKAGLRPDGKGILLPVSRAVARAQNPAKAAAELRDEILNIKRELK
                     LR"
     gene            4662..5273
                     /gene="pyrE"
                     /locus_tag="PL000_16515"
     CDS             4662..5273
                     /gene="pyrE"
                     /locus_tag="PL000_16515"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNC90537.1"
                     /translation="MTELNSNLASLADGLLSAGCIKFGEFTLKSGLKSPIYIDLRQII
                     TYPSLLEQVGAAYLPLLKELKFDRLAGLPYAAIPIATAVSLQGNYPMIYPRKEVKAYG
                     TKAEIEGEYHAGETALVIDDLATTGGSKFEAIEKLTAVGLAVKDVVVLVDRQSGAKES
                     LEQAGYSMHAVLTIRQMLDHWESTGKVEKDKIEATRKFLEQNG"
     gene            complement(5642..6604)
                     /locus_tag="PL000_16520"
     CDS             complement(5642..6604)
                     /locus_tag="PL000_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90538.1"
                     /translation="MKPDKLEQAMELILSGNKRKALPILSRLVQEEPRNESAWVCLYE
                     CVDDLPKKTYCLKKILAINPDNQHARAELERISRSNTAIISLEQPPVIVVRGQTQLPI
                     QKTSTIDIIRYQFMKNPIRMWMIVILIMFLILNTGAFIFRWYTLRTVAVDHPLMPPLR
                     TTQSPYPMNLTPEYLYYATQESLPMVQANQIQIIIPETAQPQVSPMQQSLDEYLTAIE
                     QENWEAAYDHTCTAIQQVIHSPKEMRDRVFFEHSTLPVSHEILPPIDRPNRLLFTLTG
                     TTWSSRTFEVSLEINTLKFCGMGVERGDLRYLLFPAGGPLNIAQ"
     gene            complement(6647..7786)
                     /locus_tag="PL000_16525"
     CDS             complement(6647..7786)
                     /locus_tag="PL000_16525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC90539.1"
                     /translation="MSEKNSSKTSQPIMVGRHYPELDGIRSIAILLVIFFHCRIIAAP
                     QNPAEKVYYALVESGWIGVDLFFVLSGFLITGILLDTFKQENYFRNFYLRRILRIFPL
                     YYAVLLLFTLLAAFGLLKFFEQKSAIAQVSYWLYLQNWLALLDLAPTKIISHFWSLAI
                     EEQFYLLWPALVLFAARRNSVGRLGILTIIMAFIVRVVFVHYEKLAYFFTFSRMDTLA
                     LGACVAVLLRQHGSLGRFRTPALLLAAFSAVTVVTVGVTQKGFYGHDPEVLLYGLLPL
                     GIFFASFLTIALTSNENGALRLFLRNEHLRMIGVISYGVYIFHWPIIVLVQPMWPLVE
                     SFWVNQLGFLALITVTSIFVAWFSYRFFEKPFLQLKDKIAPVQKA"
     gene            7921..9012
                     /locus_tag="PL000_16530"
     CDS             7921..9012
                     /locus_tag="PL000_16530"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HNC90540.1"
                     /translation="MYNNYLRPFLFRLDPERAHTLTLDAVRMAGNMRLSLWLLERMFK
                     TPSKPVELFGLTFRNPVGLAAGYDKDGVAIRGLAALGFGHVEIGTVTPRPQPGNPSPR
                     VFRLLEDEAVINRMGFPSKGSEFMQLKLNPSLRPGWFERIYGFQPHNKNKFERSINKG
                     GAILGINLGKNKETPNEDAVYDYVSLVDVFSRYADYLTINISSPNTVGLRELQGRAAL
                     EKLLAHIHQQRLVSQTYHEKRLPILVKLAPDLTDAELDDAIEAILQAKMDGIIVTNTT
                     LAREGLKSVLRGETGGLSGSPLAVKSEAVLHRTLKQVNGAVPIVSAGGIMSPDDAKRR
                     LEMGAALVQVYTGLIYRGPGLAREIVKSL"
     gene            complement(9015..9632)
                     /locus_tag="PL000_16535"
     CDS             complement(9015..9632)
                     /locus_tag="PL000_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90541.1"
                     /translation="MTPKIILEGTRLTFKTDIAFALNEHPRIVGPRKYKYHSPLISGE
                     WCAFTNFPWGRGLINFEPQEEAQAMETYETWVKLFELQRYYSWIVDRFHLSTRMYQLN
                     THHKDYDFRWLEERLLPLNFRLVLLTRSPESFEAARAERLKVSGNPSQYDDLNLFIEE
                     QKLIRRLASESILPKLEIDISDNNIPAAVEKIADWMEATGGLYMP"
     gene            9756..10118
                     /locus_tag="PL000_16540"
     CDS             9756..10118
                     /locus_tag="PL000_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90542.1"
                     /translation="MDDRITIIEGPPPIFEHVNDGWALGLNESSTLSVPALTRLRTFN
                     GPALVQRCYNAWHNKSSIHLHYRSEAGLEETAPIIAARNVDTPDGHMLLLWVYLDGEN
                     VEYEVDAGDEDQQDDFPE"
     gene            10186..10956
                     /locus_tag="PL000_16545"
     CDS             10186..10956
                     /locus_tag="PL000_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC90543.1"
                     /translation="MSTLHTDLKSGAAILTLNRPSRANSFNFEMTTALMDALSTAEND
                     PQVRCVVITGGGKVFSAGQDITEMKQGGGVVSYREHLEKTYNPLILQIRRMGKPVIAA
                     VNGPCAGAAFGIALACDLRIAHSRAYFVVGFSGIALAPDSGVSLLLPKYIGLGRAQEY
                     FYSNKPITSHLAYQWGLVNQVGGFNFNALILQIAGRLAAGPREAFAAGKKAFNQAILP
                     NLEESLRYEGILQDEAGRSAEHREGVAAFLGKRLPKFD"
     gene            11181..12479
                     /locus_tag="PL000_16550"
     CDS             11181..12479
                     /locus_tag="PL000_16550"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HNC90544.1"
                     /translation="MSGQINAFEMAQKQFDGVAKQLKLDPGVAEVLRWPMREYSFRIP
                     VRLDDGSLKVFQGFRVQHNDARGPNKGGIRFAANETMDTVRALATWMTWKCAVADIPL
                     GGGKGGIIVDPSTLTVNEKEEMCRGWVRAMWKNIGPRNDVPAPDVGTTPQMMGWMMDE
                     YSRLVGQYTPGVFTGKPLGGGGSLGRTEATGYGVIYTVREAMKHLNIDGKNSIAAIQG
                     FGNVSQYAAVGFVEMLGGQVACVSCWDRHDKKSYTYSKKGGIDPRFLLTIVDEYGTID
                     KKKAQDAGYTVEDGDAWITKEANVLIPAALEGQVTADTVGKMSKGIKIVAEGANGPCT
                     PEADEHFKKNGIFNIPDFLCNAGGVTTSYFEQVQNDMNFYWSKEEVLERLDQKMTSAF
                     HGVLDRSEKEKVYMRDAAYMVAIDRVVTAMHLRGWLTGNS"
     gene            12648..13823
                     /locus_tag="PL000_16555"
     CDS             12648..13823
                     /locus_tag="PL000_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNC90545.1"
                     /translation="MSKQTFKWDDPFLFNDQLTDEERMIRDTARKYCQEKLMPRILEA
                     NRNESFDRSIMTEMGGLGFLGSTIPEQYGGAELNYVSYGLIAREVERVDSGYRSAMSV
                     QSSLVMYPIFTYGTEEQRKKYLPNLASGKWVGCFGLTEPNHGSDPASMETKAKKVDGG
                     YILHGNKMWITNSPIADVFVVWAKLDGEIRGFILEKGMKGLSAPKIEGKFSLRASTTG
                     EIVMEEVFVPDENLMPNVSGLKGPFGCLNKARYGIAWGALGAAEFCWHAARQYTLDRP
                     QFGRPLAANQIIQLKLANMMTEITLGLQGALRVGRLMDDDKSTPEMISLIKRNSCGKA
                     LEIARHARDMHGGNGVSDEFHVIRHVMNLEAVNTYEGTHDIHALILGRAQTGIQAFS"
BASE COUNT         3557 a         3438 c         3499 g         3332 t
ORIGIN      
        1 tgtttctgcg cctgccatgc agggagatgc tgtcagcgcc gcgccgttgt tgcgggcgac
       61 gagctcgaat ctggcgttgt cgatgacagt tgataaaccc acgctcaaca agacggaaaa
      121 tactgtagtt ttcacaatca cagtatcaaa tggcggacct gataacgcaa ctggcgtaaa
      181 agtgaaagat ttattgcctg cggggctgac atattcatca cacagcccgg gagaaaccta
      241 taatagtggt acaggaatct gggatgttgg aacattgaca tccggcggag tggggtccac
      301 tgactcatta actattaccg cgacagtgac agcttctttt ttgcctatta ccaacaaagc
      361 tgaagtgtcg tcttgggatg ccgggcaaac cgaccctaat tcaacccccg gaaacaactc
      421 aacaactgaa gacgatgacg ccagtacaat aatcaccact caaaatgcaa acttgagtct
      481 aaccaaaacc gtcaatacaa ccacacctat tgttggcagc aatgtagttt ttactgttaa
      541 tgttcaaaac acaggtcctt ttcaagcgac taatgtgacg gtcactgatt tgttacccgg
      601 tagtctcact tatatatctc atactggtgc tggaacctat gtgcccggaa cgggtttatg
      661 gacattacct acacctcttg gtgtgggagc aggcgcctct cttcaaatca ccgcttctgt
      721 agacgtaccc ggcccaatca ccaatactgc aacaatatcc aattcagatc aatatgataa
      781 caaccctgcc aacgatcagg ccagtattac actgaatccc gatacatctg acctaagttt
      841 aagcattgca gttaataata caactccgga taaaaacgac aatgttgtct ttactgtcac
      901 gctaaataat gcaggaccaa caactgcaac gaatgtggtt gttactgatg tatttccttc
      961 tgggttaagc aatatcatcg atgatgatgg tggtgttggt gccccttatc catataattc
     1021 aaatacttgg acaattcctt cccttccaag cggggctagt gctgtattaa atatcactgc
     1081 aaatgtaatt actcagggag caaagatata ttctgctcaa gtttggtcta gtgatcaact
     1141 tgatcccaat tccaccccgg gaaataactc cactacagaa gatgatgatg cgagtatttc
     1201 cttaattccc gaagtctctg atttaagttt gataaagaca gtggataatc ccattccaaa
     1261 taatggtgac actgtagtct tcaccatcac agtgaataat aatggacctg atattgcaac
     1321 taatgtttta gtagcagatg tgttgcccgt tccatttact tatatttctg atgatgatgg
     1381 tggcggtgct tttccctaca atgcgggtgt ctggagtatt ccatctcttg caaacggggc
     1441 aagtgccatt ttgcatatta ctgctcttac cgggccgggg actttaaata ccaattatgc
     1501 tgagataatg acaagtgacc agattgaccc tgattcaacc ccaggaaatg ggttgattgc
     1561 agaggatgat caagatggat cgccatctgc cgatttgagc ctttccatga cggtgagtaa
     1621 tccatcacct ccattaggta gtgacataac ttttgtactt acggtcaata atataggagt
     1681 tggaactgcc aataacgtat ttgtgaaaga cattctccca gcagggctgg aatggcaatc
     1741 agacaatggc gctggggcat ataacagtgg gacagggtta tggaacgttg gcagtttgac
     1801 accgagcggg gtcggttcta ctgcaaccat aagcattgtt gcaaaggtaa tgtctactgg
     1861 cgtaaaaaca aattttgcag aaatagttac cagcagtcta ttagaccccg attcgacggt
     1921 ttccaatggg tctgttaccg aggatgatga tgccaatgca gtagttaccc ctgctgctgg
     1981 tatgttggtc atcatcagcg aagttgcttg ggcgggaacc gttgccgaag gtgatgatga
     2041 atggatcgaa ctatataatc cgggcacatc tgatatcaat attaatggat ggaagttgaa
     2101 ggataattca tccataatct ccttaactgg ttcaatacca gcaggaggtt actatcttat
     2161 agaagataca tcagtggctg tttcagatgt tacacacgac ttaattttta atatgtcatt
     2221 atcaaattct ggagaatctc tggaattata tgatggatca aacatcttga ttgatactgc
     2281 taataaagat ggtggtagtt ggcctgctgg cgacgtggac acaaaaggaa caatggaaag
     2341 aattgtttct aaaacagata gtgatgacgc ttgggtgacg aataccgggt ttataaagaa
     2401 cggtaaagac agaaacggtg gcgatatttg gggaactccc aagaatcccg cacctgtaag
     2461 cacgcccaca ccgaccccgt ccggtggaat gtctattgtc attagcgaag ttgcatgggc
     2521 gggtactgct gcaaacagta gcgatgaatg ggtggagttg tataaccccg gtgcctcaca
     2581 gattgatatt accggctgga agttgataga tggcggtggc actgaaatta ttttgaatgg
     2641 caaaattgac ccgggcagct ttttcttgct ggaagataac gaaaacacag tttcagatat
     2701 tatagcaagc caacttgacg ggaatctggc tttaactaat gaaggcgaaa ctctacaatt
     2761 gttggacggt tcaaacacaa tcattgattc tgccaataat gatggtggag gctggcctgc
     2821 tgggagtaat tcaacttatg gcaccatgga acgcattgtt tcatctccag atggaaataa
     2881 tgcttggtat acaaatgcag gcattgtaaa aaatggtaaa gatgcaaatg gcggagatat
     2941 ttgggggacg ccaaaacagg gaaattctcc tatccccaca gccaccgcca cggccacgcc
     3001 catcacccaa acccccacgc gtaccctcac gcccaccaaa accgtcaccc ccacgcgcac
     3061 ggtcacccgc acgcccacag cagaacccgt gggcaggcct gtcatcaacg aattccttgc
     3121 acgtcccggc tacgattgga accgcgacgg ctttgtcgat gtgtacgatg aattcatcga
     3181 aatcaagaac atcggcatcg tagagatcaa cctcaaaggc tggaagttgg acgacgaagc
     3241 cgattcaggc tccacaccct tcaccttccc cgatctgctc ctcctgcccg gagaccgggt
     3301 cgtcttttac ggcaatgaaa cccgcatcct gctcagcgat ggcggcgaca ccgtgcgcct
     3361 gctgaacgcc agcaacaaag tgtacgacgc ctacacctac ccgcttgcca aattcaaaga
     3421 caaatccgtc tgccgcatgc ccgatggcaa cggctcatgg tacgaagact gcatccccac
     3481 gcccaacctc accaactcgc gtcagggttc cgtcccctcc atgccgggcg gctccaatgc
     3541 cgaatccgag ctttgtgatt ttcccgatac catgcccgag gattttgtct acgccgaatg
     3601 ccgcggcttc ggcggaaaca tctgggatac cttcttctgg gatcaattcg gctggctcgg
     3661 gcgcatgatc ttccaacccg catcaagcaa atgggaaatc attgtagagt aaccacaaac
     3721 ctaaaaataa aagaataaat aaaacaacaa acggtaaacc gatggatggt ttaccgtttt
     3781 gcttgtttcc ctgtatacta tcgtctcatg gaaaccttct tcacttttct cgaaaaacgc
     3841 gttgacgact catcctccct gctctgcatc ggactcgacc cccacatccc cgacctcaag
     3901 gaacccaccg ccgaatcagc cctcgacttt tgcctgactc tgatcaagca gacagctccc
     3961 tacgctgcag cattcaaacc caacgccgcc ttcttcgaag tcttcggcgc cgaagggtgg
     4021 ctggccctca aacaggtcat cgaagccatc aatgccgaat cgaaccgact gggttcgcgc
     4081 atccccatca tcctcgatgc gaagcggggt gacatcgcct ccaccgccga tgcctatgcc
     4141 aggtccgctt ttgagaacct cggcgcagat tgcatcacct tgaacccata tctcggaaaa
     4201 gactccatcg aacccttcat tcaaaattct gaaaaaggtg tcttcctgct ttgcaaaaca
     4261 tccaacgcag gttcaatgga cctgcaaaat ctgctcgtat tgccgatggg ttcagatacc
     4321 cccatgcccc tgtatattta tgtcgccaaa ctcgcacaag agtggaacga aaaaaataac
     4381 atcggcctgg tggtgggtgc cacccatccg cagatcatgg aaatgatccg cgcggccgtg
     4441 ccgaacctgt ggttcctttc accgggtgtc ggcgcacagg gcggcgaatt gcagcttgcc
     4501 ctgaaagccg gtctgcgccc cgatggcaag gggatcctgc tccctgtttc gcgtgccgtg
     4561 gcccgcgcac aaaaccccgc caaagccgcc gccgaattac gcgatgagat actgaacatc
     4621 aagagagaat tgaaactcag ataaccgaaa aggaatcatc catgacagaa ctcaattcaa
     4681 acctggcatc ccttgccgat ggcctgctct ctgcgggctg catcaagttc ggagaattca
     4741 cccttaaatc cgggctcaaa tctcccatct acattgacct gcgtcagatc atcacctacc
     4801 ccagtttgct ggaacaggtc ggtgcagctt acctgccgct gttaaaagaa ttgaagttcg
     4861 accgtctggc tggcctgcct tatgccgcca tccccattgc aacggctgtt tctctgcagg
     4921 gcaattaccc catgatctat ccacgcaagg aagtaaaggc ctacggcacc aaggccgaga
     4981 tcgaaggcga atatcacgca ggtgaaacgg ccctcgtcat tgatgatctc gccaccaccg
     5041 gcggaagcaa gttcgaagcc atcgaaaaac tgaccgcagt tgggctggcc gtgaaagacg
     5101 tggttgtgct ggtggacaga caatccggcg caaaagaatc actggaacag gcgggctatt
     5161 ccatgcatgc tgtgttaacc atccgtcaaa tgctcgacca ttgggaatcc acgggcaaag
     5221 tggaaaaaga taagatcgaa gcaacgagaa aatttttgga acaaaacggt taattcaggt
     5281 ttttatggaa ttgacctctt gcataagtaa agtgttgtag accacaaagt cacaaagtca
     5341 ctaagtcact aagctttttt ctttgagtct tcgagacttt gtgccttggt ggtaaaaaaa
     5401 tggacttatg caagagttct aataaagccc ccgccacaag aaactgaatg agagcgaaag
     5461 atctctggtg taattgcaga gaccattcgc tctctttttt tgtcacctgt cagcaggcaa
     5521 atttgtttga cttcttgcgt aagttgcaag gaatgtcttt gaaatgccat tttgaaccac
     5581 ggagttttta attgttttct ctgtgtctcc gtggttaatc tttcagactt ttgaaacagg
     5641 tctattgtgc aatattgagc gggccgcctg cggggaataa caggtaacgc aagtcgccgc
     5701 gctcgacccc catgccgcag aacttcaagg tattaatttc cagactgact tcaaaggtcc
     5761 ttgaactcca ggttgtgcct gtcagagtaa aaagcagcct gttggggcgg tctatcggcg
     5821 gaagaatctc atggctgacg ggcagggtgg agtgctcaaa aaatacacgg tcgcgcattt
     5881 ctttgggaga gtggatcacc tgttgaatgg cagtacacgt atggtcatat gcggcctccc
     5941 agttttcctg ttcgatcgca gtgagatatt catccaatga ttgctgcatc ggtgaaactt
     6001 gaggttgggc tgtttcaggg ataatgatct gaatttgatt ggcctgtacc atcggcagtg
     6061 attcctgagt ggcataatac aggtactcgg gtgtcaggtt cattggatag ggagattggg
     6121 tggttcgcag cggcggcatc agcggatggt ccacggctac cgttctgagc gtgtaccaac
     6181 ggaaaatgaa cgcgcctgtg ttcaagataa ggaacatgat cagaatcacg atcatccaca
     6241 ttcggatcgg attcttcata aactgatatc ggatgatgtc aattgtggag gttttctgga
     6301 tgggaagttg agtctgcccc cgtacgacga tgaccggcgg ctgttccaat gaaatgatgg
     6361 ccgtgttgga ccgtgatatc cgctcaagtt cggcgcgggc gtgttgatta tcggggttga
     6421 tcgccagtat ttttttcagg cagtatgttt tctttggcag gtcatccaca cattcataca
     6481 ggcataccca agccgattcg ttgcgcggtt cctcttgaac cagcctgctc aaaatgggca
     6541 aagccttccg cttgttccca gaaaggatca gttccatggc ttgttcgagt ttatctggtt
     6601 tcatattccc tcaatcatct aaaaatttgt acaaaaataa aaataactac gctttctgaa
     6661 ccggggcgat cttgtctttc aattgcaaaa aaggtttttc gaaaaaacga tagctgaacc
     6721 aggccacaaa aatggaagtg accgtgataa gcgcaaggaa tcccagttgg ttcacccaga
     6781 aactttcaac gagcggccac atcggctgca ccaggacgat gatgggccaa tggaaaatat
     6841 acacaccata actgataacc ccgatcatgc gcagatgttc attgcgaagg aagagtcgca
     6901 gcgctccatt ttcgttggaa gtaagtgcga tggtaagaaa actggcaaaa aagattccca
     6961 acgggagcag gccatacaat aacacctcgg ggtcgtggcc gtagaatccc ttttgtgtga
     7021 ctcccacagt gaccacggtc acggcgctga aggcggctaa aagaagggca ggtgtgcgaa
     7081 accgcccaag cgagccatgc tgacggagaa ggaccgccac acaagcgccc agtgcgagtg
     7141 tatccatgcg tgaaaacgtg aagaagtacg ccagcttttc ataatggacg aagaccaccc
     7201 gtacaatgaa tgccataatg atcgtaagaa tgccaagcct gcccactgaa tttcgcctgg
     7261 ctgcaaacaa gaccagcgca ggccaaagca gatagaactg ttcctcgatc gccaaagacc
     7321 aaaaatggct gatgatcttc gtgggcgcaa ggtcaagcag ggccagccag ttttgaagat
     7381 aaagccagta cgaaacttgt gcaatggctg atttttgttc gaagaacttc aacagcccga
     7441 atgcagccag aagggtaaaa aggagcagta ccgcatagta aagtggaaaa atgcgtagaa
     7501 tgcgccttag atagaaattg cgaaaatagt tttcctgttt gaaggtatcg agcaaaatcc
     7561 ccgtgatcag aaagccggaa agcacgaaga acaggtccac cccgatccat ccgctttcga
     7621 cgagggcata gtagactttt tcagcagggt tctggggggc ggcgatgata cggcagtgaa
     7681 aaaatatgac cagcaggatg gcgatgcttc tgatcccatc cagttccggg taatgccgcc
     7741 ccaccatgat cggctgggat gttttggaag aattcttttc cgacattctg cttttcgctg
     7801 ctcacaaact cccacgccat ccgatgcctt aataccgcag ccgttcgcct gcaatgggtt
     7861 aaaatgaatt ttgaactttg ttgattgtag cataggcagt gtaatcataa aaaggacttg
     7921 atgtataaca attatcttcg cccgtttctt tttcgtctcg accccgaacg cgcccacaca
     7981 ttgacattgg atgccgtccg catggcgggg aacatgcggc tctcgctctg gctgttggag
     8041 cggatgttca aaactccgtc caaacctgtt gaattgtttg gcctgacctt cagaaatcct
     8101 gtcggccttg cggcaggtta tgacaaggat ggcgttgcca tccgcggact cgctgcactg
     8161 ggatttggcc acgtggaaat cggaacggtc accccgcgac ctcaacccgg caatccgtct
     8221 cccagggttt ttcgcctgtt ggaagatgaa gcggtcatca atcgcatggg ctttccgagc
     8281 aagggctctg aattcatgca gcttaagttg aacccatccc tgcgcccggg gtggttcgaa
     8341 cgcatttatg gcttccagcc tcacaacaag aacaaattcg agcgttccat taacaagggc
     8401 ggggccattc tggggatcaa cctcggcaag aacaaggaaa cccccaatga agatgccgtg
     8461 tatgactatg tttcactggt ggatgtcttt tcaagatatg ccgactacct gaccatcaat
     8521 atcagttcgc ccaatacggt gggactgcgt gaattgcagg gacgtgctgc actggaaaaa
     8581 ttacttgccc acattcatca acagcggctg gtttcgcaaa cgtaccacga aaagcgattg
     8641 cccatccttg tcaaacttgc accggacctg accgatgccg aactggatga tgccatcgaa
     8701 gccattttgc aggcgaagat ggatggcatc atcgttacca acacaaccct tgcccgcgag
     8761 ggattaaagt ctgtgctgcg gggagaaaca ggcggcttga gcgggagtcc gcttgcggtt
     8821 aagagcgaag cggttcttca tcgcacgctg aaacaggtga acggtgcagt tcccattgtg
     8881 agcgcgggcg gaatcatgtc tcccgacgat gcaaaacgcc gccttgaaat gggggcggcg
     8941 ctggtgcagg tgtatacagg gttgatctat cgcgggccgg ggctggcgcg ggagatcgtg
     9001 aaatctttgt aagcctacgg catgtacaac ccgcctgttg cttccatcca gtctgcgatc
     9061 ttttccacgg cggcggggat gttgttgtct gaaatatcga tttcaagttt tggcagaatt
     9121 gattcgctgg caagcctgcg gatcaatttt tgttcttcga tgaaaaggtt caagtcatcg
     9181 tattgcgatg ggttgcccga aaccttgagt ctttcggcgc gtgcggcttc gaaagactca
     9241 ggagagcggg tgagcagcac caggcggaaa ttcagcggca gcaggcgttc ttcaagccag
     9301 cgaaagtcgt aatctttgtg gtgggtgttc aattgataca tacgcgtgga aagatggaaa
     9361 cgatcgacga tccacgagta gtaacgctgc aattcaaaaa gtttcaccca ggtttcgtag
     9421 gtttccatgg cctgggcttc ctcctgcggt tcaaagttga tcaaaccgcg gccccacgga
     9481 aaattggtga aggcgcacca ctcaccggaa atgagcggcg aatgatattt gtatttgcgc
     9541 ggccccacga tgcgtgggtg ttcgttgagc gcaaacgcga tgtcggtttt aaaggtcagg
     9601 cgggtgcctt cgaggatgat tttaggggtc attttgctca tgtttgtaat tatattcaca
     9661 gaataacctt acagaaaaat tctaataata ttctgaaacg attcctctgc attgttgtgg
     9721 tatattcccg aacaggtgaa accaaaggta aatttatgga cgatagaatt acgatcatcg
     9781 aaggaccccc acccattttt gagcatgtca atgatggctg ggcgcttggg ttgaacgaaa
     9841 gctcgaccct ttccgttccc gcgctgaccc gtctgcgcac gttcaacggg cccgcacttg
     9901 tgcagcgttg ctacaacgcc tggcacaaca aatcatccat tcacctgcat tatcgcagcg
     9961 aagccggtct cgaagaaacg gcccccatca tcgccgcgcg caatgtagat acccccgatg
    10021 ggcacatgct tcttctgtgg gtgtatctcg atggcgaaaa cgtggaatac gaggtggatg
    10081 caggcgacga agaccagcag gatgattttc ccgaataact gactctcaaa gtattgaccg
    10141 tcaccttgca caaggtgacg gtcgcttttt ttgaatcgag gatgaatgtc aacgcttcat
    10201 actgacctca aatctggtgc ggcgattctg acgctgaatc gccccagccg cgccaattcc
    10261 ttcaacttcg aaatgaccac tgccttgatg gatgccctgt caactgcaga gaacgacccg
    10321 caggttcgct gcgtggtcat caccggcggt gggaaggtgt tcagcgccgg tcaggatatt
    10381 accgaaatga aacagggtgg tggtgtggtt tcgtaccgcg agcatttgga gaaaacctat
    10441 aaccctttga tcttgcagat ccgccgcatg ggcaagcctg tcattgccgc ggtcaatggg
    10501 ccgtgtgcag gggcggcatt tggtattgca ctggcttgtg acctgcggat cgctcattca
    10561 cgcgcttact tcgtggtggg gttcagcggt attgcacttg caccggattc gggtgtatcg
    10621 ctccttttgc cgaaatatat tgggctgggc cgcgcacagg aatattttta ttccaacaaa
    10681 cccatcacat cccatctggc gtatcagtgg ggattggtca atcaggtggg tgggtttaac
    10741 ttcaacgcgc tgattctgca aattgcgggc aggctggccg caggtccgcg tgaggcgttt
    10801 gcggcgggga agaaagcctt caatcaggcg atcctcccca atttggaaga gtctctccgt
    10861 tatgaaggca ttttgcagga tgaagcaggc cggtcggcgg aacaccgtga gggagtggcg
    10921 gcatttttag gaaagcgcct cccgaagttc gattaggaat ttccttcaaa accagtcaaa
    10981 actcatcatt aatcagcgca aatatgcgga tttttgtcat attgacgtca ttctgattct
    11041 cgtgtaaagt ttaggggtac ttgtgattta atggattaac tttatggttc agcagattga
    11101 cataaatcgt gtttagagcg ggtaccccgt ccggggtgcc cgcttatatt ttctcaaatt
    11161 ctcatcaagg agaacaaaaa atgtcaggac agatcaatgc ctttgaaatg gcgcagaagc
    11221 aattcgatgg agttgccaag caattgaagc tggatcccgg cgtagccgag gttcttcgct
    11281 ggccgatgcg tgaatattcc ttccgcatcc ctgtgcgctt agacgatggt tcgctcaagg
    11341 ttttccaggg cttccgcgtt cagcacaatg atgcccgcgg tccgaacaaa ggcggcattc
    11401 gcttcgctgc caatgagacc atggataccg tgcgcgcgct cgccacatgg atgacctgga
    11461 aatgcgctgt ggcggatatt ccgctcggcg gcggtaaggg cggcattatt gtcgatccct
    11521 ccacacttac cgttaatgaa aaagaagaaa tgtgccgcgg ttgggtgcgt gccatgtgga
    11581 agaacatcgg cccacgcaat gacgtgcccg ctcccgatgt gggtaccact cctcaaatga
    11641 tgggctggat gatggacgaa tattcccgcc tcgtcggtca gtacaccccg ggtgtattta
    11701 ccggcaaacc gctcggcggc ggcggctctc tcggccgcac cgaagccacc ggctacggcg
    11761 tgatctacac cgtgcgcgaa gccatgaaac acctcaatat cgacggcaag aattccatcg
    11821 ccgccattca gggctttggc aacgtttcac agtatgcggc tgttggtttt gttgaaatgc
    11881 tcggtggtca ggttgcctgc gtttcctgct gggaccgcca cgacaagaag tcctacacct
    11941 acagcaagaa gggcggcatt gatccgcgct tcctgctcac catcgtggat gagtatggca
    12001 ccattgacaa gaagaaggct caggatgccg gttacaccgt tgaagatggc gatgcctgga
    12061 tcaccaagga agccaatgtg ttgatccccg ccgcgctcga aggacaggtc actgctgata
    12121 ccgttggcaa gatgtcgaag ggcatcaaga tcgtggctga aggcgcgaac ggcccctgca
    12181 cccccgaagc cgatgaacac ttcaagaaga acggcatttt caacatcccc gatttcctct
    12241 gcaacgcggg cggcgttacc acctcctact tcgaacaggt tcagaacgat atgaacttct
    12301 actggagcaa ggaagaagtg ctcgaacgcc tcgaccagaa gatgacctct gctttccacg
    12361 gcgtgctcga ccgcagtgaa aaggaaaagg tttacatgcg cgatgccgct tacatggtcg
    12421 ccattgaccg tgttgtcacc gccatgcacc tgcgcggctg gttgactgga aattcgtaac
    12481 gagtaattgg taattcgtaa ttgggagagg actcgccttt gggtgagtcc tctttttgat
    12541 atgcgattct ttcttaacct aaatctgatt ctgttttctt gagcagaatc tccccccaac
    12601 attttgatac aataccaaca ccaatctcta cctaccaaga ggctagcatg tcaaaacaaa
    12661 ctttcaaatg ggatgatccc tttctcttca atgaccagct gacagatgaa gagcgtatga
    12721 tccgcgatac tgcgcgcaaa tactgccagg agaagttaat gccgcgcatc ctcgaagcga
    12781 atcggaatga atcctttgac cgcagcatca tgaccgaaat gggcggtctg ggtttcctcg
    12841 gctccaccat ccctgaacaa tacggcggcg cggaattgaa ctatgtctcc tatggtttga
    12901 tcgcccgtga agtggaacgt gtggatagcg gttaccgctc tgcgatgagc gtgcaatcat
    12961 cactggtgat gtacccgatc tttacttatg gaaccgagga acaaagaaaa aaatacctgc
    13021 ctaatttggc aagcggcaaa tgggtcggtt gtttcggtct caccgaaccc aaccacggga
    13081 gtgaccccgc cagcatggaa accaaggcga aaaaagtgga tggcggctat attttgcacg
    13141 gcaacaaaat gtggatcacc aattccccga tcgccgatgt tttcgtggtg tgggcaaaat
    13201 tggatggcga gatccgcggt ttcattttgg aaaaaggtat gaaaggcctg agcgctccaa
    13261 agatcgaagg caaattcagc ctgcgtgcca gtaccaccgg tgagatcgtg atggaagaag
    13321 tctttgtacc cgatgagaac ctgatgccga acgtttctgg tttgaaggga ccatttggat
    13381 gtttgaacaa ggcccgctac ggaattgcat ggggcgccct gggtgcggct gaattttgct
    13441 ggcatgcggc aagacaatat acattggacc gtccacagtt tgggcggccg ttggctgcga
    13501 atcagatcat tcaacttaag ctggcgaaca tgatgacgga gatcaccctc ggtttacagg
    13561 gcgctttgcg tgtgggcaga ctcatggatg atgataagtc cacacctgaa atgatctcgc
    13621 tcatcaagcg taattcctgc ggcaaagcgc ttgagatcgc ccgtcatgct cgcgatatgc
    13681 acggtggtaa cggcgtttcc gatgaattcc atgtcattcg gcatgtaatg aatttggaag
    13741 cggtcaatac ctacgaaggc acgcatgaca tccatgcttt gatccttggc agggcccaga
    13801 cgggaattca ggctttttca taaaaa
//
LOCUS       DAOQLC010000112        13721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1652_length_13721_cov_23.036660, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000112 DAOQLC010000000
VERSION     DAOQLC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_13721_cov_23.036660"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(707..1156)
                     /locus_tag="PL000_16560"
     CDS             complement(707..1156)
                     /locus_tag="PL000_16560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HNC90546.1"
                     /translation="MTVINFYSTKDQYGGFSNFSRHPFELDGKQWLTTEHYFQAQKFP
                     GNEAYQEKIRLEKSPMVAARLGRSRAQPIRPDWEQVKDELMKAALLAKFRQNKDIREI
                     LLSTGDAQLVEHTKNDRYWGDGGDGSGKNKLGLLLMEIREKLRQEAK"
     gene            complement(1480..2106)
                     /locus_tag="PL000_16565"
     CDS             complement(1480..2106)
                     /locus_tag="PL000_16565"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC90547.1"
                     /translation="MGRGPVIEQDELFEVANRLEAEGKEVTALALLEALGRGSLTTIY
                     KHLEVWKTAKPSKVQLSNNEMPDRVKSAFMVAWREASQEAGQAVEEAREKAKEEVAAA
                     LRQFHGALEAIARLEKEREADADQIQALNEHVDKLAQDVSSLEASNAALKAQGEAQGK
                     DLERLQKELAKAQAERDESMKAAAEQKGKLASLEAQNADLMKVIGGKK"
     gene            2324..2617
                     /locus_tag="PL000_16570"
     CDS             2324..2617
                     /locus_tag="PL000_16570"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNC90548.1"
                     /translation="MELVGEDSLKHFAKKFSSARPGLDRWLKLTKAAQWKTFQDVKAT
                     FPATDYIPQNQYCFDIGGNNYRLLTAISFQLGVVTVLDFMSHADYDKKKLNTR"
     gene            2626..3045
                     /locus_tag="PL000_16575"
     CDS             2626..3045
                     /locus_tag="PL000_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90549.1"
                     /translation="MDAKYLELVEQFPLLPITNKNEHKAAKDMVLRLTARDGELSPVE
                     VGYAKVLVQLIQSYESALVGNFFDGVTGSDVLEYLLNEHQMTQTDAADIAGVSKQNLN
                     DFLKGRRGLPKDARLRLAKHFKLDAHIFELSRELTSV"
     gene            complement(3037..4776)
                     /locus_tag="PL000_16580"
     CDS             complement(3037..4776)
                     /locus_tag="PL000_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90550.1"
                     /translation="MQWSSALDDGQIMGVMSDEGRGLYRGLYWGEETRHGVLDIDAGS
                     KYHDAHELRELASKFAAVGLPLVPYQSSDTGGWHLYFYLTEWANAEEVEGTIRKYLKA
                     NSYSILAGTLEIFPSGNALRLPLQKGFGWLSSDGQLEVRREELTFGQALALFYNDLEE
                     NKRNWGEVRSLIERAISDAGAGVAGCAQERENTVSIEGLEGLYKRGIDWEKWQRGKRY
                     WQDGLTEPNQRHDAILTVGHYLWFGDEARGLRALPLQRNAGKRAELIKAWLIEKHNGH
                     SEEVIKGRWSEIEADIERAAWWTSQRALIGRNQKQYEPYQLTDRLLKRLKWLHKQTGK
                     LFTIEELEKANIERSQDARHRIAVAVAQLEAENQEIDISKVARRAKACHKTVAKHRDL
                     LCSPGGEYIAGGEGGLSGSLLPAVGSETPEEEISVQTYLPLFGESGETALLDQGVNEA
                     SFISGDESQTFKALSLIELTEFFGFKSEHEDIEPSKFVHDLSVLPSCESTVTIGVGSF
                     KVAPVVSCLAESQPESTQHQAQALRVAWQSLTPGPVLSGIQALRHATPGGIYQWSGER
                     AGSKSRGPPLASN"
     gene            complement(5230..5856)
                     /locus_tag="PL000_16585"
     CDS             complement(5230..5856)
                     /locus_tag="PL000_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90551.1"
                     /translation="MSDTARDIQTFDPSSTEGLQELFEQGLLKESLEQSQGVLEGKQG
                     VPVELAATYLGLSVSGVLKRLRKGVLPGFKVPSKRGEKWLVSYEGLPESAMDTLKDSF
                     SVPEVVLLEPEESSSLLEGLLDDAEDSSAHAQNIIEESSKRADVDRALLEELRSRNSE
                     LETKLQAASWRNGYLESKLEERDIQIKLLTDSQHKPSWWQRFKAFFVK"
     gene            6057..6383
                     /locus_tag="PL000_16590"
     CDS             6057..6383
                     /locus_tag="PL000_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90552.1"
                     /translation="MSITANKVILWQALLLLTGIALQEWYICSPHYMGFDRILQPPVY
                     LAVLSLIIPAALILKQKEGRAHWLCLMLISPYLFFSMATHMSDLSHDWKAENLFGPAR
                     LQRLVY"
     gene            complement(6419..7543)
                     /locus_tag="PL000_16595"
     CDS             complement(6419..7543)
                     /locus_tag="PL000_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90553.1"
                     /translation="MKRTFKSLLAVAGLAFIGIGFALTAAPAQAAIVCDEQGTNSSTY
                     PLDGAGAATSHAYRCKNTANGAKPSDARTQDLFNSLASMPVNVRNQLKSKNVNYYYFN
                     NRSEAITYFNGTAPFNDQVNYESITPFAQSTSRCGATGYATNRFTGVPLIAVAIYDNC
                     NYDQNTNPALYVQNPSVRKTGFHESGHAFDFSFGLGGGDFPSNRSGFVSLFNGDKTLL
                     TPSNWAQYSTGSKNNYICNLFSYASPSAFEKELGATSNGGPGGQVCQTNLVPYTYYQT
                     KTPTQIASEKLPYFMGNKQEIWAEAFVIVVEGNNTSPASFLPITDRVIGMNQSPVRSF
                     NCVRATLESYINLLTSPKNLPASDPYSLPSKGCPVPSGAL"
     gene            complement(8298..8870)
                     /locus_tag="PL000_16600"
     CDS             complement(8298..8870)
                     /locus_tag="PL000_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90554.1"
                     /translation="MSEPGGHLDTRIALSELADELQCHKQSLFKIAKRLGINPIKQRD
                     INRRNQLVAMITNEEAQLIKTEFLMRAVNSNQLGADSDLAITEDGVFYLLQLEPTHDP
                     GRIKLGFTTDLDGRLRKHRCSAPFAQCLKTWPCRRIWERTVIDAVTTGMEVLHTEVFR
                     TGCLNSAIQKADQIFALLPSVQIEQEVDAE"
     gene            complement(8986..9204)
                     /locus_tag="PL000_16605"
     CDS             complement(8986..9204)
                     /locus_tag="PL000_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90555.1"
                     /translation="MLGDIELTKEADEFKPIFDFFLVEENLEQEPPFPEEKLWNWFVV
                     DQAGNREEIGLPHIEGKDISWRFIGVRK"
     gene            complement(9308..9601)
                     /locus_tag="PL000_16610"
     CDS             complement(9308..9601)
                     /locus_tag="PL000_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90556.1"
                     /translation="MVSEEPPTFEFVLELPEHLEGPLKLPNGDTVDVGDMIEHTSLGI
                     GKLYRIATYHDELGVLLCVEFPNNQHEMLGLNFVRKVNPETGKSPGGGSSSNT"
     gene            complement(9611..11638)
                     /locus_tag="PL000_16615"
     CDS             complement(9611..11638)
                     /locus_tag="PL000_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90557.1"
                     /translation="MVSSAGLTGIKQVFSERAALVSNRESEEPGDLRLKGSPENLAMR
                     TADAQAARSAGVSDVRAAQDTNPNKHNGSAGGDQLESIQIIALDDGGKQKVLAERPKE
                     AKPQPDITPEYLRERAKQPDIWAKKFVEDMDKAEKLPAPYKDAQIAKVLKDAEAVYRP
                     RGQAEKPQAPLDQMDTGTLLAHEVKRNPAAEPVQNLHTWAKQLPEGPEKDRYSQLVRD
                     QMAELSPEAKARAERLAHVTEKIKGAGLEEGGVHQAVKPDQLVLQGRAQHQENDWEAF
                     TKLDPKQQRDIIKAFEKAGEVGKEAYEDQIQAVAESIPKGFYNVGKGLYDSAIAAGTF
                     MIEAADRPEKIPQAAQKLAENLSTTLMSGLKISEVIACYSQEVAQSGDYSRPLRDLQV
                     VTTLANERWQAMPLEKRTEKGSELIAEMGVGSVIGAADKLAKSGKLIDALEDLAKYSR
                     ELTAPGREKATRALGTFLDEVFQPKGLTTNGFEMPIPKQQRDIDDLVMLKNKGLPDTV
                     KPSEQVITKRRFAAQERAVHTLEVPEEVYSAAEKRGLSRQIVKEKLDHVAKALTDAYN
                     TLGDYSPQKHGSERSYGLKLHEVLRRQLGTDELIHTEASYKKGFPVPWGKLGSSRVDI
                     ALGEHEKPFVSLCLKTLGAVPSAQQERGWYRNLPRFDDDSVIPRIYFKLGK"
     gene            complement(11639..12148)
                     /locus_tag="PL000_16620"
     CDS             complement(11639..12148)
                     /locus_tag="PL000_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90558.1"
                     /translation="MGAGIVLGIIAALIAGGVANEVMKDPNAAFGPAILVGGAVFWFF
                     KNQEDKANEARDKPTEFLVNVGMAEAFGFVKQVLTESHMGDTWWQIRSIDPEAGKILA
                     ITSWKEYIGDQVGEATRQITLNASFYPGTENKTMLVFQWDVVSSFNRNQANQVIDQFT
                     AAVKNGLGA"
     gene            complement(12190..>13721)
                     /locus_tag="PL000_16625"
     CDS             complement(12190..>13721)
                     /locus_tag="PL000_16625"
                     /inference="COORDINATES: protein motif:HMM:NF014581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HNC90559.1"
                     /translation="DNYVTLTPEDSIKHAIVCGPTGSGKTTAVFIPNLSERLGSSVIV
                     TEATAGTETADLYYNTAGYRRMCGDNRIYYFNPDDLHSDRINPLDVVNSYAKAQELAQ
                     LVIENTTSKNNYGDDVWPKSEANLLTILIAHAAAEKQHLGYIRALLREGPDGLMPLLE
                     KSKVEQVRQEYRGFCNNSRDGFKYGVFAGLMQRLGLWVNPRIVALTEKTDIDIEGLPS
                     QLFSFYLATPAAKTHLKPIAALIFNFILDLALNKHFEKPLFLSLDEFTNFGMIPGIAE
                     KLSIIRHKRIGVMLGFQDYSQLLKVYGKDDATQMFSQPGTKFFFRPRTLEVAEKISKM
                     AGNTTVYERKMTSSGHIQERESGRPLIDPGEVLALADQEMIVFTPQCPPLKMPRFTWE
                     DYKDSMAIKPLPKIEVVVDERLVRECNELKKPAEWQQQAEEMRKKEQSEKPKPQAKPE
                     QKAQNSSKGAPKKNRNRFKERPQRPKEERREEPEKPKLRAVEPQETRTKQEKNKATSY
                     D"
BASE COUNT         2899 a         3902 c         3105 g         3815 t
ORIGIN      
        1 atctttgcct tgagcttgga attacccgcc aaaccctcta tcggtacgta agtccggccg
       61 gtaagttgag agaaagcggg aagaaactcc tgaaagcagg cagccatcgc agtggacgcc
      121 ggtgacatcg actattgaaa tctagtgaca tcgactactg aaaatgacaa taccgcctcc
      181 agctttacta ggtcttcggc cgctagcctg gtgttcaact ggggcgtttg aaccgggtct
      241 ttgcctggtt ttctcggtct tggcatggct aattcctagt tttcctatac aataatcctt
      301 acctacatac taacgcttca gcttacgttt ttaggctgct caatttttag cttacgctat
      361 aagtccacaa tattgagcca tttactattt tagcttacga tctttatcct gccttactac
      421 gtaagggcgc tgcgcggctt cgtccgtctt gtctcgttgc ccactcgccg ccgcgccgcc
      481 cgcagtagtc gcaacacccc ggcacagcac gaccctaccg ggctcccttc aaaacccagt
      541 tagtgcgagg tcgtgcggag gtagcccctt cggggcgggc ggggaaccac aagcaccgct
      601 gcaaggggcg ctttctgcac tgctcgcagg acaccttgag cgccctgggc aacacctttg
      661 ctccctagtc cagctaacac gccgggcggc tctttgaggt tctgtgttac tttgcctctt
      721 gtcttaattt ttctcggatt tccattagaa gcaagcccag cttgttcttg ccgctgccgt
      781 ctccaccatc accccaatac ctgtcattct ttgtatgctc aacaagttgg gcgtctccgg
      841 tcgatagcaa tatttcccgg atgtccttgt tctgccggaa tttggctaga agagcggcct
      901 tcatcagctc gtcttttact tgctcccaat ctggcctgat gggttgtgct cggctgcgcc
      961 ctagccttgc cgccaccatc ggcgacttct ccagccttat cttttcctga taagcctcat
     1021 tccctgggaa tttctgcgcc tggaaatagt gctctgttgt tagccattgc ttgccgtcca
     1081 gctcaaacgg atgtctcgaa aagtttgaaa agcccccgta ctggtccttt gtgctgtaga
     1141 aattgatgac cgtcatacac ctgcctaaat ctggccaacc ctccaatata catcaaattt
     1201 tttccctgtc ttgaaaaaat tgattgggaa aattcttgct ctatgcttgt tttcgaggca
     1261 cttcaagagg tctcgataca attcaagagt acacacaaaa tcggggccag agtttgcgcc
     1321 tcttgccccg attgcacact tcaaaaggag gtaaatccca tggaaatgct agttttatgc
     1381 cttttcattg tagcattgtc agtgctctcc ctcagtcgat aacctgaagg agaccgccac
     1441 gggacgccct ttctgggcgc cctgggcggt gcaatttact tactttttgc cgcctattac
     1501 cttcatcaaa tcggcgttct gagcctccag ggatgcaagc ttgccctttt gctctgccgc
     1561 cgccttcatg ctctcgtccc gctccgcctg ggcttttgct agctcctttt gcagccgctc
     1621 taggtctttg ccttgcgcct cgccctgagc tttcaaggct gcattgctcg cttccaggct
     1681 gctcacgtcc tgggccaact tgtccacgtg ttcattcagc gcctggatct gatcggcgtc
     1741 cgcttcccgc tccttctcca gtctcgctat cgcctccagc gccccatgaa actgcctcaa
     1801 ggcggcggct acttcctctt tcgccttctc tctcgcttct tcgactgcct gccctgcctc
     1861 ctgcgaggct tcccgccagg ccaccatgaa agcgctcttt acccggtccg gcatctcgtt
     1921 attactcaat tgcaccttac tgggctttgc cgtcttccac acctccaggt gtttgtagat
     1981 ggtcgtcaga ctcccccgcc ccaatgcctc cagaagtgcc agcgccgtta cttctttgcc
     2041 ctctgcctct agccggttcg ccacctcaaa tagctcgtct tgctcaatca ccggacctct
     2101 gcccatcttc agcacctctc acccctctcg acgctcaaaa tataccacga taattaccgt
     2161 aattatctta ttacgattct tattattatc attattacta cagttgcgcg tcaccttccg
     2221 ccaaaaatcc cccgaaaccc ccaccagtcc acgccgccgc ccacccttga cggagtactc
     2281 atttttgagt acaattaagg cgtcaccagt catcaggagc ccaatggagt tagtcggcga
     2341 agacagcctt aaacactttg ctaaaaagtt ctccagcgcc cgccctggac ttgatcgctg
     2401 gctcaagctc accaaagccg ctcagtggaa gaccttccag gacgttaaag ccaccttccc
     2461 agctaccgac tacatccctc aaaaccaata ctgctttgac atcggaggca acaactacag
     2521 gcttttgacc gctatctcat ttcaacttgg ggtggtcact gtcctggact tcatgagcca
     2581 cgccgattac gacaaaaaga agctcaacac taggtagaga taatcatgga tgctaaatac
     2641 ttggagctgg tcgagcaatt ccctctcctt ccaatcacca ataaaaatga gcacaaagcg
     2701 gcaaaagata tggtccttcg cctgactgcc cgtgatggag agctttcccc ggttgaagtt
     2761 ggctacgcta aagtcttggt tcagctcatc caatcctatg aatccgccct tgtcggcaac
     2821 ttcttcgatg gcgtcaccgg ctccgatgtc cttgaatacc tgctcaatga gcaccaaatg
     2881 actcaaaccg atgctgccga cattgccggg gtttcaaaac agaacttgaa cgatttcctc
     2941 aaaggtcgcc gcggtctgcc taaagatgcc cgccttcgcc tggccaaaca cttcaagctc
     3001 gatgcccata tctttgagct gtcccgagaa ttgacctcag tttgaagcca ggggaggacc
     3061 ccggctttta cttccggctc tctctccaga ccattgataa attccccccg gcgtggcatg
     3121 ccgcaatgcc tgaataccgg agagcacggg ccccggtgtc aagctttgcc aagcaacccg
     3181 cagggcttga gcttgatgct gggtgctctc tggttggctt tctgccaggc atgaaacaac
     3241 gggggctacc ttgaacgaac caaccccgat agtcacggta ctctcacaag agggcagtac
     3301 actgaggtca tgcacgaact tacttggctc tatatcttca tgctcgctct tgaagccgaa
     3361 aaactcggta agttcgatca ggctgagcgc cttgaacgtc tggctctcat caccgcttat
     3421 aaagctggct tcgttgactc cctgatccaa aagggcagtt tcaccactct caccgaacaa
     3481 gggcaaataa gtctgaactg aaatttcttc ttctggggtt tcagaaccaa cagccggaag
     3541 caaagagcct gacaagcccc cctcgccccc cgctatatat tcgcctcccg ggctgcaaag
     3601 aagatccctg tgcttggcaa cagttttgtg acaagccttc gccctcctgg ctaccttcga
     3661 tatgtctatc tcttggttct cggcttctag ctgggccacg gctaccgcta tccggtgccg
     3721 ggcgtcctgg ctcctctcta tgttcgcctt ctccagctcc tctatcgtga atagcttgcc
     3781 ggtttgtttg tggagccact tcagccgctt cagtaacctg tctgttagtt gatagggttc
     3841 atattgcttc tggttccggc ctattagcgc cctctggctt gtccaccatg cggccctttc
     3901 tatgtccgcc tctatctccg accaccggcc cttaatgacc tcttctgagt gcccgttatg
     3961 cttctctatt agccacgcct taattagctc cgcccgcttt cctgcattcc tttgcagtgg
     4021 gagcgccctt aaccctctcg cttcatcccc aaaccacaaa taatgcccaa cagtaagaat
     4081 cgcgtcatgt ctctgattag gctccgttag cccgtcttgc cagtagcgct ttcctctttg
     4141 ccacttctcc cagtcaatgc cccttttgta cagcccctcc aaaccctcga tagatactgt
     4201 gttttcacgc tcctgggcgc agccggcaac gccggcgccg gcatcactta ttgccctctc
     4261 tatcaggctt cggacttctc cccaattcct cttgttttct tccaggtcgt tgtagaagag
     4321 cgccaacgct tgcccaaagg tcagttcctc tcttctaact tctagctgcc cgtctgaaga
     4381 tagccacccg aagccctttt gtaatggtag tctcagggca ttgccacttg ggaatatctc
     4441 cagcgttcca gccaaaatgc tgtagctgtt tgccttcagg tacttcctta tcgtcccttc
     4501 tacttcctcc gcgttcgccc attcggtgag ataaaaatag aggtgccagc cccctgtatc
     4561 gctcgactgg taagggacta gaggcaatcc gactgcagca aatttggacg cgagctcccg
     4621 tagctcgtgc gcgtcatggt atttgctccc ggcgtctata tccaagaccc catgccgggt
     4681 ttcttcgccc cagtacagcc ctcgataaag cccgcgcccc tcgtccgaca tcactcccat
     4741 gatctggccg tcgtccaggg cgctactcca ctgcatccac cttctttgtc ctggtctctt
     4801 ccagatcgag ccctgacgcc tgggatatag cgcgcatata tcccccgcca ccgcatccag
     4861 tcccgggggc aaagatttaa ttttctcagc taacttttgc tctcgggcta caactgccca
     4921 ctgagcggct ttctctgccg tttccttcgg tcctttacta gaccgacaac ttgacgaacg
     4981 gactcaagcc ctctattctg tcagcagttt caaaaaccca tccgccaaga ttgttttcga
     5041 aacacccaca aaactgaaaa ctcagatcca gtctaggatt tcaaatgcgc cagctaccaa
     5101 gctggtgttt tttgttgtcg gcttttttcc tccaaatgtc tcagcgatac cggccgtata
     5161 tacaagagat agacaccgcc gatggtatca ccgactgtca ataatagagt agttgggctt
     5221 tgatcgctac tattttacga aaaaagcctt gaatcgctgc caccatgagg gcttatgctg
     5281 gctgtcggtg aggagtttga tctgaatgtc gcgctcttca agtttcgatt ctaggtagcc
     5341 gtttctccaa gaagctgctt gtagctttgt ttcaagttct gaattacggc ttctaagttc
     5401 ctctaagagt gctctatcta catcagctct tttcgaggac tcctcaatta tattttgcgc
     5461 atgagcggag gagtcctccg cgtcatcaag gagtccttcc aatagagacg aggactcctc
     5521 cggctctaaa aggactacct cgggcacaga aaaggagtcc ttgagggtat ccatcgcact
     5581 ttcaggcagg ccttcataag agactagcca cttctcgccc ctcttagagg ggactttaaa
     5641 gcctggaaga acaccttttc gcagtctttt aagcacgcca ctaacagaaa gaccgagata
     5701 agttgcagcc agttcgacag gcacgccttg ctttccctca aggacgcctt gtgattgctc
     5761 caaggactcc ttgaggagtc cttgttcaaa cagttcttgt agtccctcgg tagaggacgg
     5821 gtcaaaggtc tgaatatcgc gggcggtatc actcatggca aggagtatag gtggaatcga
     5881 aaagcctgtc tagctcttgg gctctcaaga catcgagtgc atatacaaac aggactgcta
     5941 ttagttgacc agtctattat cttcaccgaa aaaagttacc ggtagtagta cgtatttgca
     6001 aacagccaag attagttagg ctagaactag tatgggctga aaatgggtgt acgggtatga
     6061 gcatcacggc taacaaagtt attctctggc aagcactctt gcttttgact ggaatcgcgc
     6121 ttcaagaatg gtacatatgc tcaccgcact acatgggatt cgacagaatt ttacagccac
     6181 cggtttatct agcagttctt tctctaatca ttccggctgc tctaattctc aaacaaaaag
     6241 aaggtcgggc acattggctg tgcctgatgt taattagccc ttacttgttt tttagcatgg
     6301 cgacacatat gtcagattta tcacatgact ggaaggcaga aaatttgttt ggtccagcaa
     6361 ggcttcagag attggtctat taaacagagg ggtagcaata gctacccctc tgcattaatc
     6421 agagtgcccc actaggaacg ggacacccct tcgaagggag cgaataaggg tcacttgcag
     6481 gaaggttctt gggtgaagtg agcaagttga tgtaagactc caaagttgct cgaacacaat
     6541 tgaaagagcg aacaggactc tgattcatac caattaccct atcagtgatt ggaaggaagc
     6601 tggctggcga agtgttgtta ccctcaacaa cgataacgaa tgcttcagcc cagatttctt
     6661 gcttattgcc catgaaatag ggcaatttct ctgaggcgat ttgggtaggt gttttggttt
     6721 ggtagtaggt gtatggaaca agattggttt gacagacctg cccaccaggc ccaccgttgc
     6781 tagtggctcc aagttccttc tcaaaagccg acgggctggc ataagagaac aggttacaga
     6841 tgtagttgtt ctttgaaccg gtggaatact gagcccagtt agaaggagtc agtaaggttt
     6901 tgtcaccgtt gaacaaagag acaaaccctg agcggttaga aggaaaatct ccaccgccga
     6961 gcccaaaaga gaaatcaaaa gcatgaccac tttcatggaa gccggtttta cgaacactcg
     7021 gattctgaac ataaagggcg ggattggtgt tctggtcgta gttgcaatta tcgtaaattg
     7081 ccacagcaat caacggtacc cctgtgaatc ggttagttgc ataaccggta gcaccacacc
     7141 gggaagtact ttgtgcaaac ggagtgatgc tctcgtagtt cacctgatca ttgaaaggag
     7201 ctgtaccgtt gaagtaggta atagcctcgc ttctgttgtt gaaatagtag tagttgacgt
     7261 tcttggactt cagttgattt cggacgttga ccggcattga ggcaagagag ttgaacagat
     7321 cttgcgttct agcatcagac ggtttggctc cgttggctgt attcttacat ctgtaggcat
     7381 gagatgttgc agcgcctgca ccgtcgagag gataagtgct gctgtttgtt ccttgctcat
     7441 cgcaaactat ggccgcctgg gccggagcgg cagtaagggc aaagccaatg ccaatgaatg
     7501 ccaacccagc gacagccagg aggcttttga atgttcgttt catgttcttt acctttcggt
     7561 taatggttgt ttgggttatc ccttcatggt tttcaacgca ggcgacaacc gactaaacga
     7621 cactgccgct taacttgatt gcttgtattt ctttgcctgt gtggcttggt aagttttgcc
     7681 cggttgaaaa ctaaagagat aacgtcatga gaatcgggca aattagaggc tgaaaaccga
     7741 ctttgtaagc gattggaaaa ttaaagggct ctgagaaaac cccgaaaccc aagccgctta
     7801 acaacttcct aaagtttagg attcagaaag ggccgttcct agctagcgct aagagcagat
     7861 aaatggagcc tattagaggg ggctaggagg tcgctggtca aaactttgga ccgggtttgg
     7921 tatgaaaggc tatccctacc ggtagcgttt agccaaaagc gccactttgc agccggtcaa
     7981 aaagcgccac caccatatgt agcgccagga caagaagcca agcgcaaaat tggccgctgt
     8041 ggtctaccca agcccccacg aaaagccgct ttcagagcca gccaagatgc aataaaacat
     8101 aaaagatatt atccgcagca attatcacga cattggcact tcttcacatc ttaagccact
     8161 ctaagtacaa aacacctgaa tctcgatact gtctctacta caacgcaatg attagcggct
     8221 tctggtaagg ctgctcttga ttgggattga ttggaaagtg cactgtcctg gagcttcggc
     8281 ttcgggagtt tttgcttcta ctcagcgtct acttcttgct cgatttgcac ggatggtaaa
     8341 agcgcaaaaa tttgatcagc cttttgaatc gcggaattta gacaacctgt tctaaaaacc
     8401 tctgtatgta agacttccat gccagtagtg acagcatcta ttacggtcct ctcccaaata
     8461 cggcgacagg gccaagtctt aagacattgg gcgaaaggtg ctgagcatct gtgttttctg
     8521 agtcgtccat caagatctgt agtaaacccg agctttattc gccccggatc gtgggttggc
     8581 tctagctgta gtaaataaaa aacgccatcc tctgttatcg ccaagtctga atctgcgcca
     8641 agttggttgg aattgacggc gcgcattaga aattcagtct tgatgagctg tgcctcttcg
     8701 tttgtaatca ttgctacaag ctgattacga cgattgatgt ctcgttgttt aattggattg
     8761 attccaagtc ttttggcaat tttaaaaagg ctctgcttat ggcactgcaa ttcatcagca
     8821 agctcagaca gtgcaattct agtgtctaaa tgtccgcctg gttctgacac gaattctaaa
     8881 tgccttatct aaatctgctc aagccctccg ggattgtagc aaagtctgat aataatcatt
     8941 atgtcttagt gaaagagccc ccaccggggc tcttttccgt aggtactact ttcgcacacc
     9001 aataaagcgc cagcttatgt ccttgccctc aatgtgcggc aaaccgattt cctctcgatt
     9061 tccagcttga tcaacaacaa accaattcca taacttctct tcgggaaatg gtggctcctg
     9121 ctctagattt tcttcgacca agaagaaatc aaaaattggc ttaaactcat cggcttcttt
     9181 cgtaagttct atgtcaccaa gcatttcgag cccatctaca ctcgaattgg taatggtgcc
     9241 tatcaaggta ttcttgtgaa acaacttaaa cgtgctactg ctcatacttc aaaaaccgca
     9301 attgccatta tgtattactg gaagagcccc caccggggct ctttcccgtt tcagggttga
     9361 ccttccttac aaagttaaga cctaacattt catgctgatt gtttggaaac tctacacaaa
     9421 gtagaacacc taattcatcg tggtaagtgg ctatccgata taacttacca attcccaagc
     9481 ttgtgtgctc gatcatgtct cccacatcga cggtatcgcc gttcggcagc ttcagaggac
     9541 cttcaagatg ctctggtagc tctaacacaa actcaaatgt tggcggttct tccgaaacca
     9601 ttagcaatcc tcactttcca agtttgaaat aaatacgcgg aattacagaa tcatcatcaa
     9661 aacggggcaa atttctgtac cagccgcgtt cttgctgagc agatggcacc gcccctagtg
     9721 tcttcagaca caagctgacg aacggttttt cgtgctctcc taaggctata tcgactctgg
     9781 aagagccaag tttgccccat ggaacaggaa agcctttttt gtaacttgct tccgtatgaa
     9841 tcaactcatc cgtgccgagt tgacgcctta aaacttcgtg caacttaagg ccataagaac
     9901 gctcagatcc atgtttctga gggctgtagt caccgagagt attgtatgca tcggtaagtg
     9961 ctttggctac gtggtcaagt ttttctttta ctatttggcg gctcaaaccc cgtttctctg
    10021 cggcgctata gacctcctcc ggtacctcca gagtgtgaac agctctctct tgagctgcaa
    10081 atctgcgctt agttatgacc tgctcacttg gtttgaccgt atccggcaaa cccttattct
    10141 tgagcattac caggtcgtct atgtcccgct gctgcttcgg tatcggcatc tcaaagccgt
    10201 tcgttgtcag cccttttggc tgaaacactt catcgaggaa tgtgcctagt gcccgcgtgg
    10261 ctttctctct accaggcgcg gttagctctc ttgagtactt agccaagtcc tctagggcat
    10321 ctatgagctt gccagatttc gccagcttgt cagccgcccc tatcactgaa ccgacgccca
    10381 tttccgctat cagctcgctg cctttctccg tgcgcttctc taggggcatt gcttgccagc
    10441 gctcattagc caaggttgtt actacctgca agtccctcag cggtcggctg tagtcaccgc
    10501 tctgcgctac ttcctggctg taacaggcta ttacttctga gatcttcagc ccgctcataa
    10561 gggtggtcga tagattctct gccagcttct gtgccgcctg cggtatcttc tccggtctat
    10621 ccgccgcctc tatcatgaat gttccagccg ctattgcgct gtcatagagc cctttgccga
    10681 cgttgtaaaa gcctttcggt attgactccg ctactgcctg tatctggtcc tcataggctt
    10741 ctttgccgac ttcgccagcc ttctcaaacg ccttgattat gtccctctgc tgcttcggat
    10801 cgagcttggt aaaagcctcc cagtcgtttt cttggtgctg cgctctgccc tgcaacacta
    10861 gctggtcggg cttcactgcc tggtgtacgc caccctcctc tagccccgcc ccctttatct
    10921 tctcggtcac atgcgccagc cgctcggctc tggctttcgc ttccggcgac agctccgcca
    10981 tctggtcccg cacaagttgg ctgtaacgat ccttctccgg tccttccggt agctgctttg
    11041 cccatgtatg gagattttgc acgggctccg ctgccgggtt gcgcttcacc tcatgggcta
    11101 gtagtgtacc ggtgtccatc tggtctagcg gcgcttgcgg cttttctgct tgccccctgg
    11161 gcctgtaaac cgcttcggcg tctttcaaga ccttggctat ttgcgcgtct ttgtatggtg
    11221 ccggtaactt ctcggctttg tccatgtcct ctacgaactt cttagcccag atgtctggct
    11281 gttttgctcg ctctctcaag tactcaggcg ttatgtccgg ttgcggcttt gcttccttcg
    11341 ggcgctccgc tagtaccttt tgcttgccgc cgtcgtccag ggcgattatc tgtatgctct
    11401 ctaactggtc gcccccggcg ctcccgttgt gcttgttcgg gttggtgtct tgcgccgccc
    11461 tcacgtcgga gaccccggcg gacctcgccg cctgggcgtc cgccgttcgc atcgccaaat
    11521 tttcagggct cccctttagc ctcaggtcgc ccggctcttc gctctctctg ttggacacca
    11581 gggcggccct ctccgaaaat acttgcttta ttcccgttag ccccgcgctg gaaaccacct
    11641 aagcacctag cccgttcttc actgctgctg tgaactggtc tattacttgg tttgcctggt
    11701 tcctgttgaa actcgatact acatcccact ggaatacaag catagtcttg ttctcagtcc
    11761 ctgggtagaa cgaggcgttc agggttattt gccgggtcgc ttctcctacc tggtcgccta
    11821 tgtattcctt ccagctcgta atggctagaa tcttgcccgc ctccgggtca atgctcctaa
    11881 tttgccacca tgtatccccc atgtggctct ctgttagcac ctgtttgacg aacccgaaag
    11941 cctccgccat gcctacattt actagaaact ctgtcggctt gtccctggct tcatttgctt
    12001 tgtcttcctg atttttgaag aaccagaaga ccgccccacc taccagtatt gccggtccaa
    12061 atgcggcgtt cgggtctttc atcacctcat tagccacacc tccggctatc agtgccgcta
    12121 ttatccctag aactattcca gcgcccattt ccctctcctt gcctgcttct tcgcggctcc
    12181 ctcacgtacc tagtcgtatg aggtggcttt gtttttctcc tgcttggtcc ttgtctcctg
    12241 tggctctacc gcccttagct tgggcttttc cggttcctct cgccgctctt ctttcggtct
    12301 ttgcggtctt tctttgaacc tgttgcggtt cttctttggg gctcccttgc tgctattctg
    12361 ggctttctgc tcgggctttg cctgaggctt cggcttctcc gactgctctt ttttgcgcat
    12421 ctcttcagcc tgttgttgcc actctgctgg cttcttcagc tcgttgcact ctctcaccag
    12481 gcgctcatct accactacct ctatcttcgg cagtggctta attgccatcg agtctttgta
    12541 gtcttcccat gtgaatctgg gcatcttcag cggcggacac tgcggcgtga atacaatcat
    12601 ttcctgatcg gcaagcgcca gaacctcgcc gggatcgata agcggacgcc cgctctcgcg
    12661 ttcctgtatg tgcccgctgc tggtcatctt ccgctcgtac acggttgtat ttcctgccat
    12721 cttcgatatt ttttctgcta cttccagcgt ccttggcctg aaaaagaact tcgtgccagg
    12781 ctggctaaac atctgcgttg cgtcgtcttt tccgtacacc ttgagtaact gcgaatagtc
    12841 ctgaaaccca agcatcaccc ctattctctt gtgccgtatg atgctcagct tctccgctat
    12901 ccctgggatc attccgaagt tcgtaaactc gtccaggctc agaaatagag gcttctcaaa
    12961 gtgcttgttc agcgccaggt caagtatgaa attgaagatt aacgccgcta ttggcttcag
    13021 gtgcgtcttt gctgccgggg tcgccaggta gaaactaaag agctggcttg gtagcccctc
    13081 tatgtctatg tccgtcttct ccgtcagcgc cactatcctg gggttcaccc ataaccctaa
    13141 cctctgcatc agcccggcaa atactccgta cttgaagccg tcccggctgt tgttgcaaaa
    13201 gccccggtac tcctgcctta cctgctctac tttgctcttc tccaaaagcg gcatcagccc
    13261 gtctggacct tccctaagta gcgcccggat gtagcctaga tgctgctttt ctgccgctgc
    13321 atgtgcaatc agtatagtta gtaagttcgc ctccgacttg ggccaaacgt cgtccccgta
    13381 gttgtttttg cttgtcgtgt tctctattac aagctgggct aattcttgcg ccttcgcata
    13441 cgagttaacg acatccagcg ggttaatcct gtcgctgtgt aggtcgtcag ggttgaagta
    13501 ataaatccgg ttgtctccac acatgcggcg ataaccggca gtgttgtaat agagatccgc
    13561 tgtttcggtt ccggctgtgg cttccgtcac tatcaccgat gagccaaggc gctccgatag
    13621 gttcgggatg aatacggccg ttgtcttgcc gcttcctgta ggtccgcaca ctatggcgtg
    13681 tttaatgctg tcttccggtg tcagcgtgac gtaattgtca t
//
LOCUS       DAOQLC010000113        13645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1666_length_13645_cov_23.899117, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000113 DAOQLC010000000
VERSION     DAOQLC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1666_length_13645_cov_23.899117"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PL000_16630"
     CDS             complement(<1..245)
                     /locus_tag="PL000_16630"
                     /inference="COORDINATES: protein motif:HMM:NF016175.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90560.1"
                     /translation="MFQKRIIIFANGELPNKEKARALLREDDFIIAADGGTRHAIELG
                     RTPNIIIGDLDSLPVNLEPSTLNIELMQFPADKNETDL"
     regulatory      complement(272..379)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(410..2392)
                     /locus_tag="PL000_16635"
     CDS             complement(410..2392)
                     /locus_tag="PL000_16635"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HNC90561.1"
                     /translation="MTTFRSRILESINDANLQTALDNNTERRLKGRAAAFESIPDWRD
                     RRLRANKIRADVIDNLDAYLEKFIAKNEANGVIVHRAKDAAEAIQIVLGIARSITNHH
                     LPTTKTLIAKSKSMVSEEIALNHALEKENMQVIETDLGEYIVQLRHERPSHIITPAAH
                     LKKEQVAELFHEKLGIPYTIDIPTLTATARKVLREVFLTADIGLSGVNFGVAETGGVC
                     VITNEGNGRMVTTLPPVHIALMGMERIVPNLDDLALMLSLLPRSATGQKLTVYTQLLH
                     KPIQNQTRHLIILDNGRTRLRNSPLKESLYCIRCGACLNACPVFRELGGHAYIGSDLS
                     IAPYPGPIGSVISPGLLGENYVQLAQASSLCGACKDACPVNIDLPKLLTRVRAGAVNS
                     KQSSVISGGAGLTRSTRFGLQMYTRIATHPRLFAASQKLAALTSSLFAPFSQWIHLPA
                     FTGWGYSKDFPKFAGKTFRERYVKRAESGESEIDNAYSSNPQSPVTNHQLPIGRHQFT
                     EELTALGGHVYNVENATQEIIKFLKSRNINKIHLEPNLLDEGLLRESNIEFTHEPDPQ
                     ITVGVTNAICGLADTGSVLIVDGDGSPLKGSLLPEIHVAILKTSNILPSLPDAMHLVK
                     DTRAAVFITGPSRTADIEMTLTIGVHGPKEIHVFLT"
     gene            complement(2447..3172)
                     /locus_tag="PL000_16640"
     CDS             complement(2447..3172)
                     /locus_tag="PL000_16640"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNC90562.1"
                     /translation="MPTVQLFVTCLVDSFFPQTGEAVVDILHRLGIGVDFAPDQTCCG
                     QPQFNAGLRKDARAIAEHTIRVFENTTGDIVTPSGSCAHMFRHNYPELFEGDSLWLPR
                     AKALAERTYEFTEYLVDKLNVTDLGARWDGALTYHPSCHTLRGINVDRQPRALLAKVK
                     GATLIDLPHAEECCGFGGIMSVEHPELSAEWLKRKISNLEASQAPVLVVTDAGCRMHI
                     AGGLNRQKKSQRVMHIAEVLNGK"
     gene            complement(3182..4294)
                     /locus_tag="PL000_16645"
     CDS             complement(3182..4294)
                     /locus_tag="PL000_16645"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNC90563.1"
                     /translation="MTMSHPPIKKIAISTGGGDAPGLNAVIRAAALSAIKRGWEVYGV
                     RDGFNGILSPEEYPDGGFISLTRERVRGITHLGGTILGTTNRNNPLRYPITRADGSIE
                     EVDRGDEVVRGFRLHHIDALITVGGDGSLAIASVLAKKGLRLISVPKTIDNDLDKTDV
                     TFGFDTAVGFATEALDRLHSTAASHGRVMVVEMMGRYAGWIALHSGVAGSADVILIPE
                     HPYDIHKVVEKIKEREERGSKFTIVVVAEGASPVGGAVTVVEHEAGRAERLGGIGEKV
                     AAEIHAISGKETRVVVLGHLLRGGSPTSFDRLTALRFGAAAVRALEEGQSGVMIALDP
                     PHVKYVPIEDAMGKMKMVPMDSDIVRTAYDLGVSFG"
     gene            4452..6593
                     /gene="glgP"
                     /locus_tag="PL000_16650"
     CDS             4452..6593
                     /gene="glgP"
                     /locus_tag="PL000_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNC90564.1"
                     /translation="MKFLTTPSQKFDIPKRIARLGELAYNLWWTWQPEAARLFGRLDY
                     DLWERLGHNPIRMLQEISHGRLVQAAKDQDYLTLYDALFESFDSYFTKSSWTQQAHPE
                     FDGNPVAYFSMEFGLHETLPIYSGGLGVLAGDHLKESSDLGLPLIAVGFMYGQGYFSQ
                     RISEDGWQEALNNPLTFEHLPVTLVTKNKKPVTIQIEFPDRTITAQIWEVRVGRVPLY
                     LLDSNVEGNTDFDRLLTARLYWSDLDRRVMQEVLLGVGGVRALRALDYSPAVWHMNEG
                     HASFLTLERVRELVTKGLPFADAAKKSLGQNVFTTHTPVPAGNDEFPLWLIDKYLANF
                     WPELGLTREEFVDIAKHTHPRGDAYSMPVLALKLSGGSNGVSELHGEVSRGMWNFLWP
                     EKQEKDVPIQHVTNGVHTANWMARRMNVLFEKYFGNDWYDHLDEPNFWSKLDSIPDQE
                     LWAVRLHLKRKLTFYMRERVRERWRQGGFHPVQVVSSGVMINPYALTIGFARRFATYK
                     RANLILSDIERLLDIINRPNMPVQIIFAGKAHPADEPGKELIQKVYRTVKKAETGGRL
                     VFVEDYDMNLARYLVQGVDVWMNTPRRPLEASGTSGMKAALNGVLNFSVLDGWWREAY
                     NGTNGWAIGEDKEYPSNEVQDAADAEDLYHTLEHEIIPLYYNRDPKEISHEWISRMKD
                     TMKTCIPQFSTRRMVKEYVEKFYAPALNRKP"
     gene            6609..6953
                     /locus_tag="PL000_16655"
     CDS             6609..6953
                     /locus_tag="PL000_16655"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC90565.1"
                     /translation="MFPEITVTELGEKLKTEEKFILLDIRELPELNQAKIEDSRLEVT
                     PMSRMGREGVAALSEEVRSQQVPVYVICHHGSRSVQVTGWLLQQGFKNTFNVRGGIDE
                     YARRVDPSVGFY"
     gene            7375..8994
                     /locus_tag="PL000_16660"
     CDS             7375..8994
                     /locus_tag="PL000_16660"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HNC90566.1"
                     /translation="MKNNPNKLWVPVFVLGWLFDFLFWKKSPGINFAIFAVSCLLVAF
                     YLLLSDGLRPNRASLILLPLFGFFAAVTFFRAEPMTTFLAVTFTLLVMTTLAMTYLGG
                     RWYLYTIGDYLGKGLQLLGSMLARPVTFTGDVRKLQAEAGIQPPKRNVWPVVRGIVIA
                     VPIIAIFSSLLSSADVVFGQRLTDLLNWFNLENLPELIFRFIYIIIIGYALAGVVLHA
                     GIESKDEKLLNEDKPFIPPFLGFVESTIVLGSVVALFAVFVVIQFQYFFGGQANINIE
                     GYTYSEYARRGFGELVTVAFFSLVMLLTLTSITRRETEAQRRIYSGLGVALVVLLLVM
                     LFSAFRRLGLYEAAYGFSRLRTYTHVFLVWIGLLLVVTIVLEILHKERMFTFAALVAS
                     FGFAISLPILNVDAFIVEQNIRRGDLQAQTEASVSRVQEEEVDAQYFVELSDDAVPAL
                     VKAYLNPKLPDPVHEKIGAALACINQQRTYDGGRESSWQSFHFSSFNADRALASISEE
                     LKNYEVVDLDFPPTVILPSGEDFPCSTYYFD"
     gene            9122..9679
                     /locus_tag="PL000_16665"
                     /pseudo
     CDS             9122..9679
                     /locus_tag="PL000_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
     gene            complement(9803..10618)
                     /locus_tag="PL000_16670"
     CDS             complement(9803..10618)
                     /locus_tag="PL000_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNC90567.1"
                     /translation="MFDLSNARFAFLDIETTGLSPWFGDRICQIAIVLTEGKRIKSTL
                     DLLINPGRGLSPAASHINGLDESKLSAAPMFEEITDQLEAALKDAVVVCHNAKFDIQF
                     LDNEYRKLGRSIEYPNLIDTLLLAREYFDLPSYSLHHLAEDFSISRNSQGSRAMADAL
                     TAKNLFFAMMDTLKPNGKPLDDYIGIYNSPAWPEEGVYLPTELSEAIHSGRRLFIKYM
                     DKDGEISERWISPNQVIGLADYIYLQAYCHTREAERTFRLDRIIEVAMEIEKE"
     gene            11095..13074
                     /locus_tag="PL000_16675"
     CDS             11095..13074
                     /locus_tag="PL000_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNC90568.1"
                     /translation="MKKTMYWLTSVIVTITLTLGSFSAVGASPLAASKTYYVSLTGSD
                     TNAGTATAPFKTFTKAVSMLVAGDVLQVYGGTYTQQLNVKKSGTAAAPITVQPVAGQK
                     VIIDGGMTLDRDIILSGSYINISGFETVSATDFCVDVTGSNISVKNFYIHNCFRFGAR
                     IKGKYVTFEGSTLHSNVLENTGGVNTTGGWAGTLRVGLGGENITLKNNTIYNNYGEGI
                     GVGQGRFVNIYNNVVHDNYSQNIYVNNSTDVNVENNFVYSVDPTYYRSGAPANCIGMA
                     DEYYSGWGAQLARVRVINNIAAFCKRGIGYTYSENTDGGMDTVTIAYNTVWGSTEAGI
                     VAINQPTKTRNSVISNNIVQQSDGRLTDIQVGVGISYSNNFWVSPAKTFKSGSGDKTG
                     DVKLAAAPGLTFESFRLSSSSPAIAGAVSMGIAQDFAGNSRGPSYDMGALQFTGIVSA
                     TATAAPALPTATQPIGMTATPTRQVTATSTPIWGFPTAVPSATKTATPVVAPSTATAT
                     PIVQVSPTFTPTGVVPTATVPVSTQPSSETVFDDKNGNLVYSVGWSDVAAKQAYGGSY
                     KQTTQSGSTVSITFTGQSFSVLYKGGPSYRKMDVFVDGALVATLDENLTVSTYQPRWD
                     YPGQLAAGTHTLMLVFVSTPGVANNNGSVDAVIVR"
     gene            complement(13154..13477)
                     /gene="trxA"
                     /locus_tag="PL000_16680"
     CDS             complement(13154..13477)
                     /gene="trxA"
                     /locus_tag="PL000_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNC90569.1"
                     /translation="MTDLQYVTEADFQEEVVGSTMPVLVDFTATWCQPCKMIEPIVKQ
                     LAEEWDGKVKVVKLDADQNPNVLMQYGVMGIPTLMLFKGGEIKERLTGFQPKEKLAAK
                     VTPHV"
     gene            13571..13642
                     /locus_tag="PL000_16685"
     tRNA            13571..13642
                     /locus_tag="PL000_16685"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13603..13605,aa:Lys,seq:ttt)
BASE COUNT         3120 a         3516 c         3516 g         3493 t
ORIGIN      
        1 agatcggttt cgtttttatc ggcgggaaat tgcatgagtt caatgttcaa ggttgaaggt
       61 tcaaggttga caggcaaaga atcaaggtcg ccaatgatga tgttgggtgt tcttcccagt
      121 tctatggcat ggcgtgttcc gccgtctgcg gcgatgatga agtcgtcttc acgcagcagg
      181 gcgcgggctt tttccttatt gggcaattcg ccgttggcaa agatgatgat gcgtttttga
      241 aacaaaaaat cctccgaagg cggaggatgg ctataggtga gtcaagagtc cctccgctgg
      301 tattaaccag atcaggtaat gcgggtcgag cgaagtacgc tcctctcagc ctttcgaaaa
      361 ggctccccgt tcaattgtga agtcagtata tgtccgcgat gggcggatgt caagtcaaaa
      421 aaacgtggat ctctttcggg ccatgcacac cgatggtcag ggtcatttca atatcagccg
      481 tgcgcgatgg ccctgtaatg aagactgcgg cgcgtgtatc tttcacaaga tgcatggcat
      541 cgggcaaaga tggcagaata ttggatgtct tcaaaatggc gacatgaatc tcaggaagca
      601 acgacccctt gagcggactg ccatccccgt ccacgatcag aacggacccg gtatctgcca
      661 gcccgcaaat ggcattcgtc accccgacag tgatctgcgg gtcgggttca tgggtaaatt
      721 caatgtttga ttcgcgcaaa agaccttcgt ccagcagatt tggctcaaga tgaatcttgt
      781 tgatgttgcg cgatttcaaa aacttgataa tttcttgcgt ggcgttctca acgttgtaga
      841 catgcccgcc cagcgcggtg agttcttcgg tgaattgatg cctgccaatg ggcaattggt
      901 gattggtaac tggagattga ggatttgacg agtatgcatt atcaatctct gattctccgc
      961 tttctgccct cttcacatat ctttctcgaa acgtcttccc tgcaaatttt ggaaagtcct
     1021 tgctgtagcc ccagcctgtg aatgcgggca ggtgaatcca ctgcgagaac ggggcgaaga
     1081 gagaggatgt cagcgcagcc aatttttgtg acgccgcaaa cagccttggg tgagtcgcga
     1141 tgcgggtgta catctgcaaa ccgaatctgg tcgagcgagt caatcccgcg ccgccactga
     1201 tcactgacga ctgtttactg ttcactgctc ctgccctcac acgagtcaat aatttcggca
     1261 gatcaatatt caccgggcag gcgtctttgc acgcgccgca caatgacgaa gcctgcgcga
     1321 gttggacata attttcgccg agcagcccag gcgaaatgac cgagccaatg gggccaggat
     1381 acggcgcaat ggaaaggtca ctgccgatgt aggcatggcc gccgagttca cgaaaaacgg
     1441 gacaggcatt caagcacgcg ccgcagcgga tgcaatacaa cgattctttg agcggcgaat
     1501 ttcgcaggcg tgttcgtccg ttatcaagga tgatgaggtg gcgcgtttga ttttgaatgg
     1561 gtttgtgaag cagttgggtg tagacggtga gcttttgccc cgtggccgag cgggggagta
     1621 gtgagagcat gagcgcgaga tcatcaagat ttgggacaat gcgttccatg cccatcagcg
     1681 caatgtggac aggcgggagt gttgtgacca tgcgcccgtt gccttcattg gtgatgacgc
     1741 acacgccgcc cgtttcagcc acaccaaagt tgactccgct caagccgatg tcggcggtga
     1801 ggaaaacttc gcgcagcact ttgcgtgcgg tggcggtgag ggtggggata tcgatggtgt
     1861 agggaatgcc gagtttttcg tggaagagtt cggcgacctg ttcttttttg agatgcgccg
     1921 caggggtgat gatgtgactt ggccgctcgt gccgcaattg cacaatgtat tcgccaagat
     1981 cggtttcgat gacctgcata ttttcttttt ccaatgcatg gttgagcgcg atctcttcac
     2041 tgaccatgct tttggatttt gcgattaagg tcttggtagt tggtaagtgg tgattggtaa
     2101 ttgacctggc aattcccaac acaatttgaa tcgcctctgc cgcatctttt gcgcggtgga
     2161 cgatgactcc attggcctcg ttctttgcga tgaatttttc aagatacgca tcaaggttgt
     2221 caatcacatc cgcgcggatc ttgtttgcgc gtagccgcct gtcgcgccaa tcagggatgg
     2281 attcaaatgc ggcggcgcgg cctttcaaac ggcgctcggt gttgttatcc agcgcagttt
     2341 gcaggtttgc gtcgttgatt gattcgagaa tacgagagcg aaatgtagtc ataggagttt
     2401 gaacgtttaa atgttgtaac gttggaacct tctaacgtcg gtatattcat tttccattca
     2461 acacttcagc gatatgcatt acgcgctgac ttttcttttg acgattcaac ccgcccgcaa
     2521 tgtgcatgcg acaacccgca tcggtcacga ctaaaacggg agcctgactc gcttccaagt
     2581 tgctgatctt gcgctttagc cattctgctg aaagttcagg gtgctccaca gacatgatgc
     2641 cgccaaagcc gcagcattct tcggcgtgcg gaaggtcgat cagcgttgcg cctttgactt
     2701 ttgccagtaa cgcgcgcggc tgacgatcca cattgattcc gcgcagggtg tgacaggatg
     2761 gatggtaggt gagcgctccg tcccaccttg cgccgagatc ggtgacgttg agtttatcta
     2821 caagatattc ggtaaattca tacgtgcgtt ctgccagtgc ctttgcgcgt ggcagccaga
     2881 gcgaatcacc ttcaaagagc tcagggtaat tatggcggaa catgtgcgcg cacgaacctg
     2941 atggagtcac aatgtcgcct gttgtgtttt caaagactcg gatggtgtgt tctgcaatgg
     3001 cgcgcgcatc cttgcgaagc cccgcgttga attgcggctg tccgcaacag gtttggtcgg
     3061 gggcaaaatc cacgccaatg ccgaggcggt ggagaatatc cacgacggcc tcaccagttt
     3121 gcgggaagaa agaatcgacg agacaggtga cgaagagttg aacggtgggc atgaatccgt
     3181 tttacccgaa cgaaaccccg agatcatacg ccgtgcggac aatgtccgaa tccattggca
     3241 ccatcttcat cttgcccatg gcatcttcga tcggtacgta tttgacgtgc ggcgggtcga
     3301 gggcgatcat cacaccactc tgaccttctt ccaatgcgcg cactgcagct gcaccgaatc
     3361 gcagtgcggt gagacggtca aaggaagtgg gggagccgcc gcgcaataaa tgaccaagca
     3421 ccacaacgcg ggtttccttg cctgaaatgg catgaatttc agccgccact ttttcgccaa
     3481 tgccgcccag tcgttcggcg cggcccgctt catgctcaac cacggtcact gcaccgccta
     3541 ctggagatgc gccttcagcc accaccacga tcgtaaattt gctgccccgc tcttcgcgct
     3601 ccttgatttt ttcaacgact ttgtgaatat cgtagggatg ttcaggaatc aagatcacat
     3661 ctgccgaacc agccacgcct gaatgcaaag cgatccagcc tgcgtagcgg cccatcattt
     3721 ccaccaccat cacccgtcca tgcgaggcgg ctgtggaatg caggcggtca agcgcctcgg
     3781 tggcgaatcc gaccgcagta tcgaaaccaa aggtgacatc ggttttatcg aggtcattgt
     3841 cgatcgtttt tggcacagag atcagacgca ggcccttttt tgcaagcaca cttgcaattg
     3901 ccaatgagcc atctccgccg accgtgatga gcgcgtcaat gtggtgcaag cgaaagccgc
     3961 gcaccacttc atcgccgcga tccacttctt cgatgcttcc atctgcgcgg gtgatcggat
     4021 acctgagcgg attgttgcgg ttggtcgttc ccagaatggt tccgcccaaa tgggtaattc
     4081 cgcgtacccg ctcgcgtgtg agtgaaatga acccgccatc ggggtattct tctggtgaga
     4141 ggattccgtt gaaaccatcg cggactccgt acacttccca gccgcgtttg atggctgaaa
     4201 gggctgctgc acggatcact gcattcaaac cgggggcgtc gcctccaccg gtgctgattg
     4261 ctattttttt aatgggagga tgagacatgg tcatgaggtt gctccagtaa atatagactt
     4321 tctggttgga ttatagtcct gttgacagag tgtatgccat gtgaaaatct acactttgtt
     4381 cacattacaa tttatgcagg aaaacaaatc cgtgcaactt cgcgtcattt ttacggtatc
     4441 attctgccgc tatgaaattc ctaaccaccc cctcccaaaa atttgatatc cccaagcgca
     4501 ttgcgcgcct cggtgaatta gcctacaacc tgtggtggac gtggcagcca gaagctgccc
     4561 gcctcttcgg acgactcgat tatgacctgt gggaacgcct cggccataat cccatccgca
     4621 tgctgcaaga gatcagccac ggtcgtttgg ttcaagccgc gaaagaccag gattacctca
     4681 ccctgtacga tgcccttttt gaaagttttg attcttattt cacgaagtca tcctggaccc
     4741 aacaggccca ccccgaattc gatggcaacc ctgtggccta tttctccatg gaatttggtt
     4801 tgcacgagac cctgcccatt tattcgggcg gccttggcgt actggcaggc gaccacctga
     4861 aagaatcctc cgatcttggc ctgccattga tcgctgttgg attcatgtac gggcagggat
     4921 atttctccca acgcatcagt gaagatggct ggcaggaagc gctcaacaat ccgctcacct
     4981 ttgaacacct gcctgtcaca cttgttacca aaaacaagaa acctgtcacc attcaaatcg
     5041 aatttcccga ccgcaccatc accgcccaaa tctgggaggt gcgtgttggc cgtgtgccgt
     5101 tatacctcct cgattcaaac gttgaaggca acaccgattt tgaccgtctg ctcactgccc
     5161 gcctgtactg gtctgacctt gaccgccgcg tcatgcagga agtattgctc ggcgtagggg
     5221 gcgtgcgtgc cttgcgcgcg ctggattatt cccccgcggt ctggcacatg aacgaaggcc
     5281 acgcatcttt cctgaccctt gagcgggtgc gtgaactcgt gacaaagggc ctcccctttg
     5341 cagatgccgc caaaaaatcc ctcggacaga acgtcttcac cacccacaca cctgtccctg
     5401 caggcaatga cgaattcccg ctctggctta ttgataaata cctcgccaac ttctggcccg
     5461 aacttggcct cacccgcgaa gaatttgttg atattgccaa gcatacccat ccgcgcggtg
     5521 atgcctactc catgcctgta ctggctttga aactgtctgg cggcagcaac ggcgtttccg
     5581 aattgcatgg cgaagtctcc cgcggcatgt ggaatttcct ctggcctgaa aagcaggaaa
     5641 aggatgtccc catccagcat gttaccaacg gcgtccacac cgccaattgg atggcacgcc
     5701 gcatgaacgt tcttttcgaa aaatatttcg gcaacgattg gtatgaccat cttgatgaac
     5761 ccaatttctg gtcgaaactt gattccattc cagatcagga actgtgggcg gtgcgtttgc
     5821 acctcaaacg caagttgact ttttacatgc gtgagcgtgt ccgcgagcgc tggcgtcagg
     5881 gtggattcca ccccgtgcag gtggtttcct ccggtgtgat gatcaatccc tacgcattga
     5941 ccatcggttt tgcccgccgt ttcgccacct acaagcgtgc caatctcatc ctttctgata
     6001 ttgagcgctt gctcgatatc atcaatcgcc cgaacatgcc tgtgcagatc atctttgccg
     6061 gcaaagcgca ccccgccgat gagcctggca aggaattgat ccaaaaggtc tatcgcaccg
     6121 tcaaaaaagc cgaaaccggc ggacgcctcg tcttcgtgga agattacgac atgaaccttg
     6181 cccgctacct cgtccagggc gtggatgtgt ggatgaacac cccccgtcgt ccgctcgaag
     6241 ccagcggcac ctcaggcatg aaagccgccc tgaatggcgt gctgaatttc tccgtgcttg
     6301 atggctggtg gcgcgaagcc tacaacggca ccaacggctg ggccattggc gaagacaagg
     6361 aatacccctc gaacgaagtt caggatgccg ccgatgcaga agacctctat cacaccctcg
     6421 aacatgagat cattccgctc tactacaacc gtgacccgaa ggaaatttca cacgagtgga
     6481 tcagccgcat gaaagacacc atgaagacct gcattccgca attctccacc cgccgcatgg
     6541 taaaggaata cgtggaaaaa ttctacgctc ctgcactcaa tcgtaagccg taaatcttta
     6601 gaggtcccat gtttcctgaa atcaccgtta ccgaacttgg cgaaaagctc aagaccgaag
     6661 aaaagtttat cttgctcgac atacgcgaac taccggagct gaatcaagcc aaaatcgaag
     6721 acagcaggct tgaggtcacg ccgatgagcc gtatgggtag ggaaggggtg gcggctctct
     6781 ccgaagaagt ccgctcacag caggtacccg tctacgttat atgtcatcat ggcagccgaa
     6841 gtgtgcaggt gacaggctgg cttttgcagc agggtttcaa aaataccttt aacgtccgcg
     6901 gcggaattga tgagtatgcc cgtagggtgg acccttctgt aggtttctat taatcccaaa
     6961 agggcacagt cacactgtgc cctttctgtt ttaaagccaa actgccaccc ttgcggatgg
     7021 cagtttgggg tttgcgctgt ggcgtccttt agatcttgag gagggatgcc acagcgctgg
     7081 gtttactact actattcata agcgacatca cctcctcctt tcattaggat ggcaattcaa
     7141 tttgtttgcc cccatccagt gtcgaaagta tgtcataaaa taaaatgaat gtcaataggg
     7201 catttttgat tctcacaaat ttgtaatatt aaatcggcgc tttttggcaa aatatgatac
     7261 cttttgagag tctccgccag actttcacgg ctattctgtt cccattgcgc gggtcatacc
     7321 gaggcatatt caaatcatcg tcctgagtta cggcaaagga cgttaggaga actgatgaaa
     7381 aacaatccga ataaactgtg ggttcctgta tttgtacttg ggtggctgtt cgatttcctg
     7441 ttttggaaaa aatcacctgg cataaatttt gccatttttg cggtttcgtg tctgttggtg
     7501 gcgttctacc tgcttttgag cgacgggctt cgcccgaatc gcgccagcct gattctgctt
     7561 ccgctctttg gctttttcgc cgctgtcaca tttttccgcg ccgagccaat gaccaccttc
     7621 ctggccgtga cattcaccct tttggtgatg accaccctcg caatgaccta ccttggcggg
     7681 cgttggtatt tgtacacaat tggtgattat cttggcaaag gcttgcagct gcttggaagc
     7741 atgctggcac gccccgtcac ctttacaggg gatgtgcgca aattgcaggc cgaagcaggg
     7801 attcaacccc caaagcggaa tgtttggccg gtggtgcgcg gcattgtcat tgctgtgccg
     7861 atcattgcca ttttctcttc cctgctttct tcagctgatg tcgtcttcgg tcaacggctg
     7921 actgacctgc tcaactggtt taaccttgaa aacctgcctg agctgatctt ccgctttatc
     7981 tatatcatca tcatcgggta tgcgctggct ggggtggtct tgcatgcggg gatcgagtcg
     8041 aaggatgaga aacttctcaa tgaagataag cccttcattc caccgttcct tggctttgtt
     8101 gaatcaacca ttgtgctggg cagtgtcgtg gccctgttcg ctgtgtttgt cgtcattcag
     8161 ttccagtatt tctttggcgg acaagccaac atcaacattg aaggctatac ctactctgaa
     8221 tacgcccgcc gtggcttcgg tgaactggtg acagtggctt tcttctcact ggtgatgctg
     8281 ttgacactta cctccatcac caggcgcgaa accgaagcac aacggaggat ctactctggg
     8341 ttgggggtgg cgttggtggt gctcctgttg gtcatgctct tttcggcgtt caggcgtctt
     8401 ggcctgtatg aagcggcata tggattttca cgcctgcgca catacaccca tgtcttcctt
     8461 gtctggatag gtttattgct cgtcgtcacc attgtgcttg aaattttgca caaggagcgt
     8521 atgttcacct ttgcggccct ggttgcgtcg tttgggtttg caatttccct gcccatcctc
     8581 aatgtggatg cgtttatcgt ggagcaaaac attcggcgtg gtgatttgca ggcgcaaacc
     8641 gaagcctccg tttcacgggt gcaggaagaa gaagtggatg cccaatattt tgtggagctt
     8701 tctgacgatg ccgttccggc gcttgtgaaa gcctacttaa accccaaact gcctgaccct
     8761 gtgcatgaga agatcggcgc ggcgctggct tgtatcaacc agcagcgcac ctatgacggt
     8821 ggacgtgaat cttcgtggca atccttccat ttctcctcgt tcaatgctga tcgggcattg
     8881 gcttcgatct cagaggaatt gaaaaactac gaggttgtcg atctggattt cccccctacg
     8941 gtcattttgc cctctggcga agattttccc tgttccacct attattttga ttagtttgta
     9001 agaaaccagt ctcattgtca gtctattcac agaatatcca aacctaaacg agccaaagga
     9061 actgacaatg gacttcaagc tgaccatctt ttctgattac atctgaccgt ggtgctatgt
     9121 cggccagggt gtggtcgacc aactgaaagc tgaatacaac gtggatgtgg agtggcgtcc
     9181 gttttacctg cgccccgata ccccgcctga aggcatggac ctgcccgatt acattctccg
     9241 tgcgcgggcc aatggcgccg atgaacgttt gcaaagcatg gcgaaaatgc atggcatgaa
     9301 cttcaactcg ccagaccgca tttacaatac ccgccttgcc catgaagcca ctgaatatgc
     9361 ccgtgaacat ggcaaagcac tcgaattcca tcgcgtggtt tttcgcaagg tgtatggtga
     9421 agccctcgat atcagcaaat gggaaatact ccgtgccgct gccgaagagg ttggcctcaa
     9481 tggggaagat atgcaaactg ttgtagaagg cggaaaatac accgctgaag tggcggggca
     9541 ggttcaatat gcccagcaga tcggtgtatc cggtgtgccg acttatgtca tcaacgagcg
     9601 ttatgccatc gttggcgccc agccgtatca ggccttcaaa aatgccctga cgcggatctt
     9661 ccaagagaag aaagattaaa atcaagacaa aataatactc accacgaagg acaactttcg
     9721 tggtgagtat gtttttaaga ggcttttgcc agccacctgc ttatttcatt ttttggtgaa
     9781 tcgcatagcg aagcaatctt ttttattcct tttcgatttc cattgccact tcaataattc
     9841 ggtctaatcg aaacgtacgc tcagcttcac gggtgtggca gtaggcttgc aggtaaatgt
     9901 aatcagccag cccgatgact tgattggggg agatccagcg ctctgaaatt tctccgtcct
     9961 tatccatata tttgatgaat agccgcctgc cgctgtgaat ggcttcgctc aactcggtgg
    10021 gcaggtatac gccttcttcc ggccaggcag gggagttgta aatgccgatg taatcatcaa
    10081 gtggtttgcc atttggcttg agcgtatcca tcatggcgaa gaagagattt ttggctgtga
    10141 gagcatctgc catagcacgc gagccttgtg aattccgtga aatgctgaaa tcttcggcaa
    10201 ggtgatgcag gctgtaggaa ggcaggtcaa agtattcgcg ggccagcaac agggtatcga
    10261 tcaaatttgg atattcgatg gaacgtccca acttgcggta ttcattatcc aggaattgga
    10321 tatcgaattt ggcattgtga cagacaacga ccgcgtcttt taacgccgcc tcaagctggt
    10381 cggtgatttc ttcgaacatg ggtgccgctg aaagttttga ctcatccaac ccgttgatgt
    10441 gagaggctgc cggggagagt ccccgcccgg ggttgatgag caggtcaagg gtcgatttga
    10501 tgcgtttgcc ttcggtcagg acaatggcga tctggcagat ccgatctccg aaccaggggg
    10561 aaagtccggt ggtctcgata tccagaaaag cgaagcgtgc attggaaaga tcaaacatga
    10621 gttgggggaa ccgccttttt gatgtgggct tatcttaccg ctttttttcg tttggggaaa
    10681 tcgtaacttt ttcctgtttt tagcgaccct gtacatacca agtcgctgat agagacaaaa
    10741 aaatcgtttc aaaaagtatt taaaaaattg atttttaagt ttaaattttc cgtcctttcc
    10801 atcacctttc aggatttggg ggagtgtctt tggcattttt tgccattatg agcattggat
    10861 aagctgtttc ttgctgattt gtaaagtgac tttgcctgtt tacagaaatg tgcagatttc
    10921 tagtactcag gtcatggctg ggagaccctt gcacgggaga ccgacatgtt aatctttatg
    10981 cacgcaaata taccgtttgc cttttgcgaa caaacgctgc aacgaagcgg caaatctgac
    11041 aaaagcctga acgcaaagaa tacaggccaa cggtgtaacg aaggagatat cgtaatgaaa
    11101 aagacaatgt actggctgac gagtgttatc gtaaccatca ccctcacact tggaagcttt
    11161 agcgcggttg gggcttctcc cctggcagca tcaaagacct attatgtttc actgaccggc
    11221 agcgacacca atgcaggaac tgccaccgct ccgttcaaaa cgttcaccaa ggccgtttct
    11281 atgcttgtgg ctggtgacgt gctgcaggtt tatggaggta cgtacaccca gcaactcaat
    11341 gtgaaaaaga gcggcacggc tgctgcgccc atcactgttc agcctgtggc tgggcagaag
    11401 gtgatcattg acggcggtat gacccttgat cgtgacatta ttttgtctgg cagctatatc
    11461 aatatcagcg gctttgagac cgtgagcgcc accgattttt gtgtggatgt gactggttcg
    11521 aatatttcag tgaagaattt ctacattcac aactgttttc gctttggtgc gagaattaag
    11581 ggcaagtatg tgacctttga aggttcaacc cttcacagta atgtgctgga gaacactggc
    11641 ggcgtaaata ccactggcgg ctgggctgga accctgcggg ttggccttgg cggtgagaat
    11701 atcaccctca aaaataacac catctataac aattacggcg aaggaattgg cgttgggcag
    11761 ggacgctttg tgaacattta caacaatgtc gtacatgaca actactcgca gaatatttac
    11821 gtcaacaatt cgacggatgt gaacgttgaa aataacttcg tctacagcgt tgaccccacc
    11881 tattaccgca gcggtgcccc tgcgaactgc attggcatgg cagatgaata ttacagcggc
    11941 tggggtgcgc agctggcccg cgtgcgcgtg atcaataaca ttgccgcatt ttgcaagcgc
    12001 ggtattggtt atacctattc cgaaaatacc gatggcggca tggatactgt gacgattgcc
    12061 tacaacaccg tttggggcag cactgaggca ggtatcgtcg ccattaatca acccaccaag
    12121 acccgcaact cggtcatttc taataatatc gtccaacaat ctgatgggcg tttgacagac
    12181 attcaggtcg gtgtaggaat cagctatagt aacaacttct gggtcagccc ggccaagacc
    12241 ttcaagagcg gatctggcga caagaccggt gatgtaaaac tggccgcggc tccaggcctg
    12301 accttcgagt cgtttcgcct gagcagcagt tcacctgcca ttgcaggagc ggtctcaatg
    12361 ggcatcgccc aggatttcgc tggaaactca cgtggacctt cctatgatat gggtgccttg
    12421 caattcactg gtatcgtttc tgccacagct acggctgccc cggcattgcc gaccgctacc
    12481 cagccgattg gtatgaccgc caccccaacc agacaggtaa ctgccacctc tactccaatt
    12541 tggggctttc ccaccgctgt accgtctgcc acaaagaccg ccaccccagt tgtagctcct
    12601 tcaacagcca ctgccacacc cattgttcaa gtttcaccca cctttacccc cactggtgtt
    12661 gtacctaccg caacggtgcc agtctctaca cagccatcgt cagaaacagt gttcgatgac
    12721 aagaatggga atttggtcta ttctgtgggt tggtcagacg tagctgcaaa acaggcttat
    12781 ggtggttcct ataagcagac cactcaaagc ggttcaacgg tttccatcac gttcacggga
    12841 caatccttca gcgttcttta taagggcgga ccgtcctatc ggaagatgga tgtatttgtg
    12901 gatggtgctc tggtggctac actggatgaa aacctgaccg tctccaccta tcagccgcgc
    12961 tgggattacc ccggccaatt agcagcaggc acccacactt tgatgctggt ttttgtcagc
    13021 acacccggtg ttgccaataa caacggctct gtcgacgctg tgatcgttcg atagcaaaaa
    13081 aacagttcag ccgatgcccg actgaactgt aatatctaaa acaaaaagag ccaggtcact
    13141 ggctcttttt gttttagacg tgcggtgtaa ctttcgccgc cagtttttcc ttgggctgaa
    13201 atcctgtcag acgttccttg atctcgccgc ccttgaacag cataagggtg gggatgccca
    13261 tgacaccgta ctgcatcagg acgttcggat tctggtctgc atcgagtttc actaccttta
    13321 ccttgccgtc ccattcttca gccagttgct tgacgatggg ttcgatcatt ttacagggct
    13381 ggcaccaggt cgctgtgaaa tccacaagga cgggcatggt ggaaccaacc acctcttctt
    13441 gaaaatctgc ctcagttaca tattgtaggt ctgtcatttt tgcctccagc ggcatagacg
    13501 ttatgtcgaa tttcaatatt acgttgacgg tcttaatttc acgtgataaa atgcagtccg
    13561 ttttagtaat gggcgcgtag ctcaatggca gagcagtggc cttttaagcc attggttaag
    13621 agttcgaccc tcttcgcgct cacaa
//
LOCUS       DAOQLC010000114        13613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1671_length_13613_cov_21.635639, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000114 DAOQLC010000000
VERSION     DAOQLC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1671_length_13613_cov_21.635639"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2093)
                     /locus_tag="PL000_16690"
     CDS             complement(<1..2093)
                     /locus_tag="PL000_16690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC90570.1"
                     /translation="MAKVSLRIYNREIEGLIDQAHINEAIAHCHHILRTFPKHLATYR
                     LLGKAYLELKRYGEAVDIFSRVLMSVPDDFVSHVGMSIIRDEENKLDDAIWHMQRAFE
                     AQPSNSAIQGELQRLYGRRDGMEPPKIRMTRGALAHMYVQGELYPQAIAEIRAVLAQE
                     PDRADMQVLLARAYFHGGQKADASDMCMQLLKRYLYCYDANCIMVDLLPATAGAVEST
                     QVYRMRVGELDPYTAFAKGSVFLANEVPDASINLERLDYKEEDAPATQSWGASSSSLG
                     LGSGASSAPMLSSSNSEPDWLKNASDTSASKSGEDIPDFLRAAGWGESTGPEQPTSFF
                     DDTPASGDLTPADIPDWLKGQVPTNVSQPAAPQPASSQSIETPDWLSGLDTGSETSSA
                     SGDIPDWLSGLGTAGAVAAASSAQSEPAPAQPGNVPDWLSGLGDAATPSEPASSIPPA
                     QPGNIPDWLSGLGDDATPSEPVSSIPPAQPGNVPDWLSGLGDEPTTTNAAQTDDVSDW
                     LSGLDSPAPVAAPTPATPSQSSDVPEWLSGLGDAEAAKSAQPDDVMEWLNGLDTPKAS
                     VAPAAAASGDVPDWLRDIDDSQTPSGNGPDSLFADAQAAPAQSDDLPAWMSGLGETSQ
                     PAASQPTSDSDLPAWMTGIPDAQPEELGQTGEATEWLHSINTDAPLSQTPAPSVESLG
                     STAQEQDDAVAWLESL"
     gene            complement(2158..3453)
                     /locus_tag="PL000_16695"
     CDS             complement(2158..3453)
                     /locus_tag="PL000_16695"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNC90571.1"
                     /translation="MRAVDIIIKKRDKQELTSQEIDFFIQGFASGEIPDYQASAFAMA
                     VLLNGMTPFETADLTLAMAKSGQVLDLSKVVDIAVDKHSSGGVGDKTSISVMPMVAAC
                     GLPVGKMSGRGLGFSGGTLDKLEAIPGYRVNLTTDEFKQQLKEKGIVLTGQSLDLAPA
                     DGKMYALRDVTGTVPSMPLIASSIMSKKIAAGAQAIVLDVKTGLGAFMQTLEDAHTLA
                     DLMVDIGRLAGRKTVALISDMNQPLGSAVGNALETIEAIELLRGHAPFDYYEHCLHVA
                     AHVLVLGQRAKDLDEGRVMAEKSITDGSALEKFRVLVQAQGGDVSYVDDISKFEQAKY
                     VEVVNAPRSGFISQVHARLVGEASVLLGAGRSKKTDPIDHAVGFLIYKKVGDQVQAGE
                     PLFAIHANDETKLAEARKAVLAAYTFSDEFVSPLPLFYG"
     gene            complement(3612..4640)
                     /locus_tag="PL000_16700"
     CDS             complement(3612..4640)
                     /locus_tag="PL000_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90572.1"
                     /translation="MTLGRIAFLGSGETSLAGGRIFETLARLIPDPLRVAILETPAGF
                     ELNASLVANRVADFLKTRLQNYKPTIDLIPARKQGTEFSPDNPDILKPLLQANLIFMG
                     PGSPTYTARQLRGTLAWDIIRARHRQGATLVFASAATISVGAWALPVYEIYKVGEDVH
                     AKEGLNLFSDFGMNVSFVPHWNNAEGGIDLDTSRCFVGLERFDQWHRLLPPENIIVGL
                     DEHSGVIMDCEQGTCDVHGVSSVTVMKNKDMEMYPTGASFSLSELGNCALPVPLEKGI
                     RPEIWEMLNKASSEEGPSEEVLKLLELRQQARVAKNFAESDRLRDQIASQGWVVQDSK
                     EGQKLIKG"
     gene            complement(4642..5100)
                     /locus_tag="PL000_16705"
                     /pseudo
     CDS             complement(4642..5100)
                     /locus_tag="PL000_16705"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(5160..6122)
                     /locus_tag="PL000_16710"
     CDS             complement(5160..6122)
                     /locus_tag="PL000_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90573.1"
                     /translation="MKRWKQILFRIFLFACLVVLFGPFLVPVPELQGLVTEAELADPD
                     SKFIEVNDVTVHYKEKGEGDTTFILLHGFGSSVFSWREVMDDFSKYGRVIAYDRPAFG
                     LTERPMPETWQENPYGMGANVELLRGLMDTLKIEKAVLVGNSAGGGVSVAFALTYPER
                     VDKLILVDPGVGGGYGPQFPAWALPVMWTPQMRHLGPLMMRDYQETLPNTITREWANE
                     NNLSAEVRQEYLNALQIKNWDRAFYELTFAPAYPELRPLLPNLTVDTFIIAGADDRLI
                     RTWYFEAISQETPAPLVLLPQCGHVPHEECPEQFMQEVLKFLEL"
     gene            complement(6159..6893)
                     /locus_tag="PL000_16715"
     CDS             complement(6159..6893)
                     /locus_tag="PL000_16715"
                     /inference="COORDINATES: protein motif:HMM:NF024107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90574.1"
                     /translation="MKIIKRVLFGLVLVVGLAVLGFVIWAETPLGPAPEALAALESDS
                     NVTVTIGDAITFEPVNIKPVTGFIFYPGGHVDYRSYAVPLREIAAQGYLVVLLPVKLN
                     LAFFDVNAGDPVFAVHPEIQQWVVGGHSLGGVASALFAKDHLDTVDGIVFWASYPADD
                     SLKNSSLKMLSIYGTNDMAGMEQFSETSALLPASTEYVVIEGGNHAQFGDYGPQPGDK
                     QATISRTEQQTQVVNAVVKFLEELVH"
     gene            complement(6880..7566)
                     /locus_tag="PL000_16720"
     CDS             complement(6880..7566)
                     /locus_tag="PL000_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC90575.1"
                     /translation="MKTNLETLNEEIISCRKCPRLVEWREEVARVKRKAYKDDEYWGR
                     PVPGFGDPKARVLVVGLAPGAHGSNRTGRQFTGDASGGFLFPALYRAGFANQARAESR
                     SDGLILKDMYITASGRCAPPENKPNPEELNTCQPFLERELQLIQPSVIVCLGKIAFDR
                     ILKITATRGLKFAHGAVHTLLPSSFINHPSSLICSYHPSQQNTLTGKLTVKMFDEIWE
                     KAKLLLNENH"
     assembly_gap    7587..7686
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7696..8748)
                     /gene="queA"
                     /locus_tag="PL000_16725"
     CDS             complement(7696..8748)
                     /gene="queA"
                     /locus_tag="PL000_16725"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNC90576.1"
                     /translation="MKTSDFDYVLPESSIAQTPADPRDSSRLLVLHRDSGNVEHRIFR
                     DLSLLLQPKDLLVLNRTRVIPARIFAKKETGGKVELLLLRRRDTVTWEALVGGKGLRV
                     GKKVVVEVEEAGRADNFLQAEIIEILEGSERLIRFSEAIEPYFPKVGNIPLPPYIHEK
                     LSDPERYQTVFAKEAGSAAAPTAGLHFTPQLLDTLQNKGVKVGYVTLHVGLDTFAPVT
                     EESPEEHKIHTEWCELPQETADLINQTRQNGGRVVAVGTTSVRTLESAASRSVIGNRT
                     SDIGAFTGPTSIYILPGYQFKVVDAMVTNFHLPKSTLIMLVSAFAGRERILDTYNLAV
                     REGYRFYSFGDAMFID"
     gene            complement(8769..9632)
                     /locus_tag="PL000_16730"
     CDS             complement(8769..9632)
                     /locus_tag="PL000_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90577.1"
                     /translation="MKISLRLLPLFFLLSACGRLDSILYTPQQTPETWLQIQPFSEFQ
                     IASQKVVFVQPSTSIIVYFLGLLTIGVGLYFLKLRDGQRSRFWWGVALLLWGIGALLA
                     GTSYEAFSYAIKCEGRTACLWTSWWEIAYLIVSVWSIDAMMLAVAFTSTEGKLRKILS
                     VYSIVNAVFYFAVVMAGAFIPVKFLISFELLLIVAAPSVIAFFVINGWRYAKHKLKSD
                     LVALGAWLWLGLTIAAYFLYLISGNTTVLWEKGFWFSENDVLHIGLIIWMIYLALVLA
                     PHVRDMNQEIS"
     gene            9734..11128
                     /locus_tag="PL000_16735"
     CDS             9734..11128
                     /locus_tag="PL000_16735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC90578.1"
                     /translation="MKLSFHGAAQTVTGSMHLVEINGSRLLLDCGLYQGKRKESIEKN
                     RNFPFDPKTIDAVILSHAHIDHSGNLPNLIKQGFSGLIYCTSATAELTDIMVRDSGHV
                     QEKQAESTSYHNAKRGEPPVDPLYTEEDAKEVEPLLQPVRYDVPFEPANGVIARLQDA
                     GHILGSASISLHLEEKRIRKHLWFSGDIGRRNMPILRDPVLPKDDVDVLMMETTYGDK
                     PHRDPEEAYNEMRLVIKRTLDRGGKVIIPSFAVGRTQELVYNLSRMYDEGDLPNVPVY
                     VDSPLAVNASKIFRKHEECYDAEMKALVHSGHPALDFKLLTYINSLEESKELNARTDP
                     MIIISASGMAEAGRIVYHIRWGIENKKNTIMIVSWQSPETLGRKLAEREPNVKIFGET
                     YRCRAEIVTIGGLSAHAGQDMLLEYASGVRQKAEYIFLVHGEDKGAIPLREKLREKNI
                     KQIYYPAMHTSVEL"
     gene            complement(11135..>11361)
                     /locus_tag="PL000_16740"
     CDS             complement(11135..>11361)
                     /locus_tag="PL000_16740"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HNC90579.1"
                     /translation="ESVARYLMLGGIILFLIGGGVYLAARFGIPLGRLPGDIRIEGEN
                     GSFYFPVTSSILVSVILSLLLNLISKLMNK"
     assembly_gap    11362..11461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11462..12486)
                     /gene="ruvB"
                     /locus_tag="PL000_16745"
     CDS             complement(<11462..12486)
                     /gene="ruvB"
                     /locus_tag="PL000_16745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012914214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNC90580.1"
                     /translation="MSKSPSSSKNSGRALDPESKPDDRVDNALRPQKLDDLIGQDQVK
                     ENLSILIDAARARGEALDHVLFYGPPGLGKTTLAHVLGNEMGVNVKVTAGPAIERAGD
                     LAAILTNLRAGDVIFIDEIHRLGRAVEEVLYPAMEDFSLDIVIGKGPSARSIRLKLPK
                     FTVVGATTRLALVTAPLRARFGAVYRLDYYDLEAMKKIISRAAAMLKVESDADGIVEM
                     ARRARGTPRIALRLLRRVRDYAQVRANGVITQKVAREALDLLQVDPLGLDDVDRRVLK
                     TIIEKYSGGPVGLNTIAASISEEQDTIMDVVEPYLLQLGFLDRTPQGRVATKSAYEHL
                     GYTYTEQG"
     gene            complement(12557..>13613)
                     /locus_tag="PL000_16750"
     CDS             complement(12557..>13613)
                     /locus_tag="PL000_16750"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90581.1"
                     /translation="FCISPQDSVIIAAMENKTYSKLFVAGFFLLLVAQLFGPKLTLKT
                     ISERFLFRLPLIQNFNSLRYTLGDQVFNEGIVGKDGWLFYSGDFSIHDYQKTSPVSKK
                     NLKEVEAILNQLNDAAMRSGGSLLVVIPPDKNTIYPQYMPDEIPVLGEVSRLEQFMQY
                     MQEHTNIQIMDLRPVLSEASQSTQVYFKSDAHWNCLGAYYASAEIMSLISISHPEVRP
                     RPLSDFEIGTMVDSTLDISKTMGLGLQEETWVLTPKFETHVEVMPAPFAENKQMRVSE
                     NSQTDLPTAVILHDSFYNECLNQLLEPYFSKTYSLHYGTSKLSDYVGLIEKEKPDVVI
                     VEFAERHIEYFFKLLTE"
BASE COUNT         3553 a         3489 c         3312 g         3059 t
ORIGIN      
        1 agcgattcaa gccatgctac ggcatcatcc tgctcttgtg cggtggagcc aaggctttca
       61 accgatggcg cgggcgtctg cgaaagcggc gcatcggtgt tgatgctatg cagccattca
      121 gtggcttccc cggtttgccc aagttcttca ggttgggcgt caggaatgcc cgtcatccag
      181 gcaggcagat cgctgtcact ggtgggttgt gacgcggcgg gctggctggt ttcacccaaa
      241 ccactcatcc aggcgggaag atcatcagat tgtgcgggag cagcctgagc atcagcaaac
      301 aaggagtcag ggccattgcc gctcggagtt tgggaatcat caatatcgcg cagccaatca
      361 ggcacatcgc cagaagcagc cgcggcaggt gcaacggatg ctttgggggt atccaaaccg
      421 ttcagccatt ccatcacatc atcaggttgt gcagatttgg cggcttcagc atcaccaagg
      481 ccgctcaacc attcgggcac atcagaagat tgcgaaggtg tggcaggggt tggagcagcc
      541 acaggagcgg gagaatcaag gccgctcaac caatctgaaa catcatcggt ctgggcagca
      601 ttggttgtgg tgggttcatc acccaatccg ctcaaccaat cagggacatt gcccggttgc
      661 gcgggtggaa tggaacttac aggttcagag ggagtagcat catcgccaag tccgcttagc
      721 caatcaggga tattgcccgg ttgtgcgggt ggaatggaac ttgctggttc agaaggagtg
      781 gcggcatcac caagaccgct tagccaatcg gggacgttgc ccggttgtgc gggcgcaggc
      841 tcagattgcg cagaactggc agcggcaaca gcccctgcag taccaagtcc actcaaccag
      901 tcaggaatat caccggaagc ggacgatgtc tcagagccgg tatcgagtcc gcttaaccaa
      961 tcgggagttt ctatggattg agatgaggca ggttgaggtg cggcaggctg cgagacattc
     1021 gtcggcacct gacctttcaa ccagtcggga atgtcagcag gggtcagatc accgctggca
     1081 ggggtatcat caaaaaagga agtgggctgc tcggggcctg ttgattcgcc ccaacccgcc
     1141 gcacggagaa agtctggaat atcttcacca gatttggatg cgctcgtatc ggaagcattc
     1201 ttcaaccaat caggttcgct attggaagaa gaaagcatcg gcgccgaaga tgcacccgaa
     1261 ccaaggccaa gggatgaaga agatgctccc cagctttgag tcgctggtgc gtcctcttcc
     1321 ttgtaatcaa gacgttccag attaatggaa gcatcgggaa cttcattcgc aagaaataca
     1381 gagcctttgg caaaagcagt gtatgggtcc aactcaccca cacgcatacg atacacttgt
     1441 gtactttcca ccgcccccgc agtggcgggt aacagatcca ccatgatgca attcgcatcg
     1501 tagcaataca gataacgctt caacaactgc atgcacatgt cggaggcatc ggctttttgt
     1561 ccgccgtgga aatatgcacg agccaacaac acctgcatat cggcacggtc tggctcctgt
     1621 gccagaacag cacgaatctc ggcaatggcc tgcggataca actcgccctg cacatacatg
     1681 tgagcaagcg caccgcgtgt catgcggatc ttgggcggct ccatgccatc acgtcggcca
     1741 tagagtcttt gcaattcacc ctgaatggca gaattggaag gctgtgcttc gaaggcacgc
     1801 tgcatatgcc agatggcatc gtccagtttg ttctcttcat cacggatgat gctcatcccc
     1861 acatgcgaaa caaagtcatc gggcacagac atcaagacac ggctgaaaat atccacagcc
     1921 tcgccgtagc gtttcagttc aagatacgcc ttgccaagca ggcgataggt tgccagatgt
     1981 ttggggaagg tccttagaat atggtggcaa tgtgctatgg cttcattgat gtgagcctga
     2041 tcaatgagtc cttcgatctc gcggttgtaa attctcaaag aaacttttgc cattaccgtt
     2101 ttcctgatta attaaggttg cctgatttgg gggtaattca cgaggctata cgctatttta
     2161 cccgtaaaac agcggcaatg gcgatacaaa ttcatcgcta aatgtgtatg ccgcgagcac
     2221 cgccttgcgc gcttcagcca atttagtctc atcattcgca tggatcgcga acaacggctc
     2281 tcctgcctgc acctggtcgc ctactttttt gtagatcagg aagccaaccg catggtcaat
     2341 tggatcggtc tttttggacc tccccgcccc aagcaggacc gaggcctcac ccacgagtct
     2401 ggcgtggacc tgtgaaatga atccgcttcg aggagcattc accacctcga catatttagc
     2461 ctgctcaaat ttggaaatgt catccacata cgaaacatcc ccgccctgag cctgaaccag
     2521 cacacggaat ttctccagcg cactgccatc tgtgatggat ttctcggcca tcacgcggcc
     2581 ttcatccaaa tcttttgcgc gttgccccaa aaccaacaca tgggcggcga catgtaaaca
     2641 atgctcataa taatcaaacg gggcatgtcc gcgaaggagc tcaatggctt cgatggtttc
     2701 aagggcattc cccaccgccg aacccagtgg ttgattcata tcggagatca gggccacagt
     2761 tttacggcct gcaaggcggc caatatccac catcaaatca gccagtgtat gtgcatcttc
     2821 cagggtttgc atgaatgcac cgagacccgt ttttacatcc aaaacaatgg cctgtgcgcc
     2881 cgccgcgatc tttttgctca tgatggagga tgcgatcaac ggcatggaag ggacggtccc
     2941 cgtcacatcg cgcaaggcgt acatttttcc atctgcaggg gcaaggtcga gcgattgacc
     3001 tgtaagcaca atcccttttt cttttagttg ctgcttgaat tcatcggtgg tcaggttcac
     3061 acgatagccg ggaatggctt cgagtttatc cagggtgccg ccactgaacc caagcccgcg
     3121 gcccgacatt ttacccacag gcaaaccaca agcagccacc attggcatga ctgaaatgct
     3181 ggtcttgtca cccaccccgc ccgaggaatg tttatccacg gcaatatcca ccaccttgga
     3241 aagatcaagc acctgccctg attttgccat cgccagagtc aagtcggcgg tttcaaaggg
     3301 ggtcatacca ttcagcaaaa cagccatggc aaaagcagaa gcctgataat cgggaatttc
     3361 cccgctggca aatccttgaa tgaaaaaatc tatctcttgc gaggtcaatt cctgcttgtc
     3421 tcgttttttg atgatgatgt ctacggcacg catgaggcca actccttatg atttccttct
     3481 ggaatatttt atcgcagata agcagagacc gccctgcctc ttaataataa agcccgaaat
     3541 aaaaacaaca aaataaaaag agttgcatca aaacgatggc aactcttttt ataaaagcct
     3601 tgaaacacga attatccttt aatcagcttc tgaccttctt tgctgtcttg caccacccag
     3661 ccttgtgatg cgatctggtc acgcaaccga tctgactcgg cgaaattctt tgcaacgcgg
     3721 gcctgctgac gaagttcaag cagcttcaat acttcctctg aagggccttc ttctgaagaa
     3781 gctttattca gcatttccca gatttcgggt cggattccct tctccaacgg tacaggcagg
     3841 gcacaattgc ccagctcgct taatgaaaaa ctcgcccctg tggggtacat ttccatgtct
     3901 ttgtttttca tcaccgttac ggagctgaca ccatgcacat cacaggtgcc ctgctcgcaa
     3961 tccatgatga cccctgagtg ctcatccaat ccgacgatga tgttttcggg cggaagtaat
     4021 ctatgccatt gatcaaaacg ctccaaccca acaaaacagc ggctggtatc caggtcaatg
     4081 ccgccttcgg cgttattcca atgcggaacg aaagaaacgt tcatgccaaa atcggagaac
     4141 agattcaaac cttcctttgc gtggacatcc tcgccaactt tgtaaatttc ataaacaggc
     4201 aaagcccatg cgccaactga aatggtcgcg gcggaggcaa agacgagggt cgccccctgt
     4261 ctgtggcggg cgcggatgat atcccatgcg agcgtgccgc gcaattgacg ggcggtgtaa
     4321 gtaggcgaac caggccccat gaaaataaga ttggcttgta acaggggctt cagaatatcg
     4381 ggattatcgg ggctgaattc ggtcccctgc ttgcgggcgg ggatcaggtc aatggtgggt
     4441 ttgtaatttt gcaggcgggt cttcagaaaa tcggccacgc ggtttgcaac gagggaggca
     4501 tttaactcaa agccagctgg cgtttccaga atggcaaccc gcaacgggtc agggatgagg
     4561 cgggcgagtg tctcaaagat gcgtccgccc gcaagagagg tctcgccaga gccgagaaag
     4621 gcaattcgtc caagtgtcat actaactgga tgattcgttt agtttatcta ttacgttgag
     4681 gaaagtttca ttatcaggga tatcagccat gttggagcca taatggacat aacgaatgcg
     4741 cccgtcaagg tcaacgacgc aatttaaagg catacgtccc agtttgaaga gattcacttc
     4801 ctgcttgtag agttttgcca cacggtgacc agggtcgggc aggccgatgt atggcaggct
     4861 ttcattctgc caatagcgtt gaaaggcctc cactccatcg gggccaacgg caacgatctc
     4921 cacattccgt tttgtgaaat cagaataatg atcacgcatg cgcgccaact gcgcgcggca
     4981 gtatggtcac ataaagccgc gtaaaaatgc aagcacaaca ggcttgcttc cgcggaaact
     5041 ggaaaggcgg acagtattcc catgaatatc ttcgagttca aaatcgggtg cgatttcgcc
     5101 cggggtgatg atattgagca gcattgtttt tcacactttc agtagaattt gcaaaacgat
     5161 catagctcca ggaatttcag aacttcctgc ataaactgct ccgggcattc ttcgtgcggc
     5221 acatgaccgc attgaggcaa taacaccaac ggcgcagggg tttcctgtga aatcgcttca
     5281 aaataccagg tgcggatcag gcggtcatct gcaccggcaa tgataaaagt atcaaccgtc
     5341 aaattcggca gcaacggacg caactccggg taggcaggtg cgaaagtcaa ttcgtaaaat
     5401 gcccgatccc aattctttat ttgaagcgcg ttcaaatatt cctgacgcac ctccgcgctg
     5461 aggttgtttt cattggccca ttcacgcgtg atcgtgtttg gaagcgtctc ttgataatcg
     5521 cgcatcatca atgggccaag gtgtcgcatt tgcggagtcc acatgacagg caatgcccac
     5581 gcgggaaact gcggaccgta tccaccaccg acgcctgggt ccaccaaaat gagtttgtca
     5641 acccgttcag ggtaggttag cgcaaaagcc accgaaaccc caccgcctgc tgaattacca
     5701 accagcaccg ctttttcaat tttcaaggta tccatcaggc cgcgcagcaa ttccacgttg
     5761 gcgcccatgc catacggatt ttcctgccac gtttctggca tcggacgctc tgtcagccca
     5821 aaggcaggac ggtcgtacgc aatgacacgg ccatactttg aaaaatcatc catcacttcg
     5881 cgccatgaaa aaacgctcga gccaaatcca tgcaaaagga tgaaagtggt atcgccttca
     5941 cccttttctt tgtaatgcac cgtaacgtcg ttcacctcga taaatttact atcagggtca
     6001 gcgagctcgg cttcagtcac aagtccctgt aattctggca caggcacaag gaaaggccca
     6061 aacagcacaa caaggcaggc gaaaagaaat attctaaaaa gaatctgctt ccaacgtttc
     6121 ataatcaatt caacagactt gataccaata aagaaagatt aatgcaccag ctcctcaagg
     6181 aatttcacaa cagcattgac cacctgtgtc tgctgttctg tgcgggagat ggttgcctgt
     6241 ttgtcaccgg gctggggacc atagtcgcca aattgggcgt ggttacctcc ctcaatcaca
     6301 acgtactcgg tgcttgcggg cagcaaggcg cttgtttcac taaactgttc catccctgcc
     6361 atatcgttcg tgccataaat ggacaacatc ttcaatgaac tattcttcaa cgaatcatct
     6421 gcagggtacg aagcccaaaa gacgatccca tccaccgtat ccaaatgatc ctttgcgaac
     6481 aaagccgatg ccacaccgcc aagggaatga ccacccacaa cccattgctg gatctcggga
     6541 tgcacagcaa acaccgggtc acctgcattg acatcaaaaa aagccaaatt cagttttacc
     6601 ggcagcaaca ccaccagata tccctgtgca gcaatctcac gcagcggcac agcatatgaa
     6661 cggtaatcca catggccgcc ggggtaaaag ataaaaccag ttacaggttt aatatttaca
     6721 ggctcaaaag tgatggcatc accgatcgta acagtgacat ttgaatcgct ttccaaggca
     6781 gccaatgcct ctggtgcagg gccaagcggg gtttctgccc agatcacaaa tcccaataca
     6841 gccaatccaa cgacaagtac aagtccaaac agaacacgct taatgatttt cattgaggag
     6901 caatttcgcc ttttcccaaa tttcgtcgaa catttttacg gtcaattttc cagtcaacgt
     6961 gttttgctgg ctggggtgat aggagcaaat caaagatgaa ggatgattta tgaacgatga
     7021 aggcaggagc gtgtgaaccg ctccatgtgc gaacttcaac cctcgagttg cagttatttt
     7081 gagtattcga tcaaaagcaa tcttacccag acacacaatt acactgggtt gaataagttg
     7141 aagttctctt tccaaaaacg gctgacaggt gttcaactct tctggattcg gcttattctc
     7201 tggcggggcg caacgacctg acgctgtgat gtacatatcc ttcaaaatca gaccatcgct
     7261 tctggactcc gcccgggcct gattcgcaaa cccagcccga tacaacgctg ggaaaaggaa
     7321 tccgcccgaa gcatctcctg taaattgacg tcctgttcgg ttcgagccgt gtgcgcctgg
     7381 ggcaagcccc accaccagca cacgtgcttt cgggtcgcca aaacctggta cgggtctgcc
     7441 ccaatattca tcatctttat aagccttgcg tttgacccgc gccacttcct cgcgccattc
     7501 caccaagcgc ggacatttgc ggcaggaaat gatttcttcg tttaatgttt caaggttggt
     7561 cttcattggg tttcaggttg aaggttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnttcg aactcttaat caatgaacat ggcatcgcca aaggagtaaa aacggtaccc
     7741 ctcgcgcacg gcaaggttgt aggtatccaa aattctctcc cgccccgcaa aagcgctgac
     7801 cagcatgatc agtgtggatt ttggcaaatg aaaatttgtg accatcgcat ccacaacctt
     7861 gaattggtag ccggggagaa tgtagatcga ggtgggaccg gtaaaagccc caatgtccga
     7921 ggtgcgattt ccaattactg atcggcttgc ggcactttcg agagtccgaa ctgaggtggt
     7981 gccgacagca accacccgcc cgccattttg cctggtttga ttgatgaggt cagcggtttc
     8041 ttgcggaagt tcgcaccatt cggtgtggat cttgtgctct tcaggagact cttcagtgac
     8101 gggggcaaac gtatccaaac caacgtgcag ggtgacgtac cccaccttca cacctttgtt
     8161 ttgcagtgta tccagcagtt gcggtgtgaa atgcagacct gcggttggtg cggcggcgga
     8221 gccagcttct tttgcaaaca cagtctggta acgctcgggg tcgctcaatt tttcgtggat
     8281 gtatggcggc aaggggatat tccccacctt tggaaaatac ggctcaatgg cttccgaaaa
     8341 acggatcaac cgttcagaac cttcgaggat ttcgataatt tcggcttgca gaaaattatc
     8401 cgctctaccc gcctcttcaa cttccacaac cacctttttg cctacacgca aacccttccc
     8461 gcccaccaag gcttcccatg tgaccgtatc gcggcggcgc aacagaagca attccacttt
     8521 gccgcctgtt tcctttttgg caaaaattct cgcagggatg acccgagtcc tgtttaggac
     8581 gagaagatct ttgggttgta gaagcaggct gaggtcccga aagatgcggt gttccacatt
     8641 cccagaatca cgatgcaaca cgagcaatcg cgaagaatcg cgcggatcag caggagtctg
     8701 cgcaatggag gattcgggca atacgtaatc aaaatcggat gttttcatgt aggttcgttt
     8761 cgaattattt atgaaatttc ctggttcata tcgcgaacgt gtggagcgag cacgagtgca
     8821 aggtagatca tccaaatgat gaggccgata tgcaaaacat cattttcaga aaaccaaaat
     8881 cctttttccc acagcaccgt tgtgttgcct gaaatgagat acagaaaata agcggcaatg
     8941 gtgagtccca gccataacca ggctcccaat gccacaagat cagatttcag tttgtgtttg
     9001 gcatatcgcc agccattgat cacaaaaaat gcgatcacac ttggcgcagc gacgatgaga
     9061 agcagttcaa aagaaattaa aaatttgaca gggataaaag cgcccgccat caccacggca
     9121 aaataaaaaa ccgcattgac aatggaatat acagaaagga ttttgcgcag tttcccttca
     9181 gtgctggtaa aagcgacagc caacatcatc gcatcaatgc tccagacaga aacgatgagg
     9241 taggcgatct cccaccaact tgtccacaaa caggccgtac ggccttcaca cttgatggca
     9301 tagctaaagg cttcataact tgtgcctgcc aaaagcgcgc cgattcccca tagaagcagg
     9361 gcaactcccc accaaaaacg ggaacgttgc ccatcgcgaa gtttcagaaa gtacagcccg
     9421 actccgatgg tcaacaagcc gagaaaataa acgatgatcg aagtactcgg ttgaacaaac
     9481 acgacctttt gcgaagcaat ttgaaattca gaaaacggtt gtatctgaag ccatgtttcg
     9541 ggggtttgtt gcggcgtata gaggatcgaa tccaatcgtc cacaggcaga aagaagaaaa
     9601 aagagaggaa gcaggcgcag ggaaattttc ataaaaatga cagtttgggc gttttcaaaa
     9661 agcgctcgtt gaagttcgaa tgataatgga ttaaaatgaa aagtgaaagc acgaccgtat
     9721 cacggaggca acaatgaaac tcagttttca cggggcggcc caaacggtaa ctggctcgat
     9781 gcatctggtg gaaatcaatg gttcgcgttt gcttttggac tgcggattgt atcaggggaa
     9841 aagaaaagaa tcgatcgaaa agaatagaaa ttttcccttt gaccccaaaa ccatagatgc
     9901 ggtaattctc tcccatgctc acattgacca ttcaggcaac ctgcccaact tgatcaagca
     9961 ggggttttca ggcctgatct attgcaccag tgccaccgca gaattgaccg acatcatggt
    10021 tagagattca gggcatgtgc aggaaaagca ggcggaaagc accagctatc acaacgcaaa
    10081 gcgcggcgaa ccacctgttg atccgcttta cacggaagaa gatgccaaag aggtcgaacc
    10141 gctgttgcag ccagtccgct acgatgtgcc gttcgagcct gccaacggtg tgatcgccag
    10201 gctgcaggat gcaggtcata ttcttggttc ggcttccatc tctctgcatt tggaagaaaa
    10261 aaggattcga aaacatttat ggttttcagg cgatatcggc cgtagaaata tgccgattct
    10321 gcgcgatcct gttctgccaa aagatgatgt ggatgttttg atgatggaaa ccacctacgg
    10381 cgacaaacca caccgtgacc cggaagaggc ctacaacgaa atgcgcctcg tcatcaaacg
    10441 gacacttgat cggggcggaa aggtcattat cccctctttt gcagtcgggc gcacccagga
    10501 gcttgtgtac aacctcagcc gcatgtacga tgaaggcgat cttcccaacg tacctgtgta
    10561 tgtggatagt cctctggcgg tcaatgcgtc gaaaatattc cgcaagcacg aagagtgcta
    10621 cgatgccgaa atgaaagcac tggtgcattc gggtcacccc gcactggatt tcaaattact
    10681 gacctacatt aacagccttg aagaatcaaa agagttgaat gcacgcaccg accccatgat
    10741 cataatctcc gcttcgggaa tggcagaggc aggacgcatt gtgtatcaca tccgctgggg
    10801 aatcgagaac aagaaaaata cgatcatgat cgtatcgtgg caatcacccg aaacattagg
    10861 acgaaaactg gccgagcggg agccaaatgt gaaaatattt ggcgaaacct accgctgccg
    10921 cgcagagatc gtcaccattg gcgggctgtc ggcacacgcc gggcaggata tgctgcttga
    10981 gtatgcttcg ggggtcaggc aaaaagctga gtatatattc ctcgtccatg gagaggataa
    11041 gggagcaatt ccattacgag agaagttaag agaaaaaaat ataaaacaaa tttattatcc
    11101 cgccatgcac acaagcgtgg aactgtaaaa cacattactt gttcatcaat ttcgagatca
    11161 ggttcagaag caaagacagg atcaccgaaa ccaaaatgga acttgtgaca gggaagtaaa
    11221 aacttccatt ctccccttca atgcggatgt caccaggcag gcgcccaagc ggaatcccaa
    11281 accttgccgc aagatacact cccccaccga tcaggaatag aatgattccg ccaagcatga
    11341 gatagcgcgc cacggattcc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nccttgttct gtgtaggtgt atcccaaatg ttcataagcc gatttggtag cgactcggcc
    11521 ttgcggggta cggtctaaaa acccaagctg caacagatat ggctcaacca catccatgat
    11581 ggtatcctgc tcttcgctga tggatgcggc gatggtattc aaacccacag ggccgccgct
    11641 gtatttttcg atgatggtct tcaaaacgcg gcgatccacg tcatcaaggc caagcggatc
    11701 cacctgcaac aggtcgagag cttcacgggc aactttctgg gtgatgaccc cattggcgcg
    11761 gacctgggca taatcacgca cgcggcgcaa aaggcgcagg gcaatacggg gagtccctcg
    11821 cgcacggcgc gccatttcga cgatcccatc cgcatcggat tccactttta acatggccgc
    11881 ggcacgagaa atgatcttct tcatggcttc cagatcgtaa taatccaaac gatagacagc
    11941 accaaaacgt gcccgcagcg gagcagtgac caaagccaac cgcgtcgtag cgccgaccac
    12001 agtaaacttc ggcaatttca aacggatcga acgcgccgat ggccccttgc caataacgat
    12061 atccagggaa aaatcttcca ttgctgggta caaaacttct tccacggcac gcccgaggcg
    12121 gtgaatttcg tcaataaaga tcacatcgcc agcacgcaga ttggtgagga tggccgcaag
    12181 gtcacctgca cgctcgatgg cggggccagc cgtcactttc acgttgacgc ccatttcatt
    12241 ccccagcaca tgcgcaaggg tggtttttcc cagaccaggc gggccataaa ataaaacatg
    12301 atccaatgcc tcgcctcgtg cccgagccgc atcgatcaaa atggacaaat tttccttgac
    12361 ctgatcctgg ccgatcaaat cgtccaactt ttgcggacgc agggcgttgt ctacgcggtc
    12421 atcgggtttg gattcagggt caagggctcg ccctgaattt tttgaagagg aaggggattt
    12481 gctcatgggg agattatacc ctgcgggtag aagctatcaa taaacaacga tccgttttca
    12541 gttaagcgcg aagcggctat tccgtcagca gtttgaaaaa gtattcgata tgtcgttctg
    12601 caaattcaac aatgaccaca tcgggctttt ccttttcgat caggccaaca taatctgaaa
    12661 gtttcgaagt gccatagtgc agggaatatg ttttgctgaa atacggctcc agcaactggt
    12721 tcaaacattc gttgtaaaac gaatcatgca aaatgaccgc agtgggcaaa tcggtttgcg
    12781 aattctctga aacgcgcatc tgtttatttt cggcgaacgg ggcgggcatc acctcaacat
    12841 gggtttcgaa tttcggggtc aagacccatg tttcctcctg caaaccaagc cccattgttt
    12901 tggaaatatc aagcgtggaa tcgaccattg tgccaatttc aaaatcggat aaagggcgcg
    12961 gtcgcacttc gggatgactg atactgatca acgacatgat ctccgctgag gcataatacg
    13021 ccccaaggca attccaatgc gcatcagact tgaaataaac ctgcgtggac tgactggctt
    13081 cactcagcac agggcgcagg tccatgatct gaatgttggt atgctcctgc atgtattgca
    13141 tgaattgctc aaggcgcgag acctcaccaa gcacgggtat ttcatcgggc atgtattgcg
    13201 gatagatcgt gttcttgtct ggcgggatga ccaccagcag gctcccgccg ctgcgcatgg
    13261 cagcatcgtt aagttgattc aggatcgctt ccacttcctt caaattcttt ttactgacgg
    13321 gggaggtctt ttgataatca tggatgctga aatcaccaga gtagaacagc catccatcct
    13381 tgcccacgat cccttcattg aagacctgat cgccaagcgt ataacgcaag ctgttgaaat
    13441 tctggatcaa tggcaggcgg aataaaaaac gctccgagat cgttttgagc gtcaattttg
    13501 ggccaaaaag ctgcgccacc agcagcagaa agaagccagc gacaaatagt ttggaatagg
    13561 ttttattttc catcgcggca ataataacag aatcttgagg cgagatacaa aaa
//
LOCUS       DAOQLC010000115        13587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1676_length_13587_cov_25.378067, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000115 DAOQLC010000000
VERSION     DAOQLC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1676_length_13587_cov_25.378067"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1165
                     /gene="pstC"
                     /locus_tag="PL000_16755"
     CDS             224..1165
                     /gene="pstC"
                     /locus_tag="PL000_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC90582.1"
                     /translation="MKQENMTAENKNFRPLNLQKKLRPAESAIQVLLFLAGVLSIFVT
                     IAIVYELGKEAMLFFTDPDVTLAKFLGTTKWQPGIGQYGIWALVSATLVTSFIAISIS
                     LPLGLAAAIYLSEYASFKSRSILKPILEVLAGIPTVVYGYFALLFVTPVLKSMIGDQL
                     QVYNMLSAGIVMGIMILPLISSMSEDALSAVPRSLREAAYAMGATKVETSLQVVVPAA
                     LSGIGAALIVGISRAVGETMIVAVASGSGPNFSFNPLDAAETMTGHIARISGGDLSYN
                     SIDYTSLFAIAMMLFLLTLVLNLISQWVVSRFRERYE"
     gene            1170..2405
                     /gene="pstA"
                     /locus_tag="PL000_16760"
     CDS             1170..2405
                     /gene="pstA"
                     /locus_tag="PL000_16760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC90583.1"
                     /translation="MNMNQHYPQDREFRRMLDARYRVATLWQALFLSALLIAIVSLMA
                     LIYNVVDGAFGYIAYEYKKDPAQFTSTPIANLTQEELAAILKENLSSGAYKKLNNEQP
                     VAERSQADLFNLFMERLVQLDTKATWSMTDSLMNGEDIRAEVAEKYPKAQLEFRSWLT
                     PQFLVTPMSSKAEFAGVRTAVLGSLWLVGIAILFALPIGTGAAIYLQEYAPKNWFTAI
                     IQTNINNLAGVPSIVYGMLGLAIFVRVLEPLTSGALFGVTDSNGRTILSAGLTMGVLV
                     LPLIIINAQEAIKAVPDSLRQAAYGVGATRWQTVWYHVLPNSLPGILTGSILALSRAL
                     GETAPLIVVGASTFISLDPSGPFSKFTALPIQIYQWTTRAQSEFHAIAAGAIIVLLVL
                     LLSLNATAVLLRNRFQRRW"
     gene            2430..3200
                     /gene="pstB"
                     /locus_tag="PL000_16765"
     CDS             2430..3200
                     /gene="pstB"
                     /locus_tag="PL000_16765"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC90584.1"
                     /translation="MEKNYAIESNKLSIHYGNFTAVRDVSIKIEPQKITAIIGPSGCG
                     KSTLLRSLNRMNDFVQGCHVTGELLLQGQNLYGKNIDPVEVRRKIGMVFQKPNPFPKS
                     IYENIAWGARINNFKGKMDDLVEQSLRSAALWDEVKDKLQQSAYALSGGQQQRLCIAR
                     TIAIQPEIILMDEPASALDPISTLRIEELMQELKKNYTIIIVTHNMQQAARASDFTAM
                     MMIDEQRAGGVIEFDETKKIFTRPQDKRTEDYVTGRFG"
     gene            3233..3688
                     /locus_tag="PL000_16770"
     CDS             3233..3688
                     /locus_tag="PL000_16770"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNC90585.1"
                     /translation="MNKPKVIFLCTGNSARSQMAEGLLRALAGEHFEVFSAGTEPKGR
                     ILPEVQAVMREVGIHMEGQFSKSVMEYLGRAVFAHVVTVCADAEENCPAVFLNMGTHE
                     HWPFDDPAKFAGDDEQRITHTRRVRDQIEERLRLWLTGQGITPADYRHT"
     gene            3727..4419
                     /gene="phoU"
                     /locus_tag="PL000_16775"
     CDS             3727..4419
                     /gene="phoU"
                     /locus_tag="PL000_16775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC90586.1"
                     /translation="MIRKTFESEIQLVKDEVLLLGSMVEHAIIGSVEALKKRDIKASE
                     KLIAEDKEINKKRFAIENQLMILIATQQPMAHDLRLLASTMEIISELERMGDYAKGIA
                     NINIRMGEETLLKPLIDIPRMAQKGADMLHRALTAFVNEDVQAANAIPVEDDEVDALY
                     NQIYRELMMFVIQDPKTIERANWLLWVAHNLERVADRVTNICERTVFIATGEFGEIKS
                     SDDEFWKGQKGS"
     gene            complement(4486..5247)
                     /gene="pstB"
                     /locus_tag="PL000_16780"
     CDS             complement(4486..5247)
                     /gene="pstB"
                     /locus_tag="PL000_16780"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC90587.1"
                     /translation="MDATQPAFTIQNMDFYYSGAKKTLSNINLEIQPQKVTALIGPSG
                     CGKSTFLRCLNRMNDTIPGTHVDGKILLKGEDIYAPAMDVVTLRQRVGMVFQKPNPFP
                     QSIFDNVAFGPRVMGMDVNLNEVVEKSLTAAALWDEVKDNLKSDAMSLSLGQQQRLCI
                     ARVIAVQPEVILMDESTSALDPIATLRVEELIADLKKDYTIVIVTHNMQQAARVSDHT
                     GFFWLGELVEFAPTNQMFTVPKEELTEAYITGRMG"
     gene            5394..6269
                     /locus_tag="PL000_16785"
     CDS             5394..6269
                     /locus_tag="PL000_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC90588.1"
                     /translation="MKRAAFFFLLVSFLLVSCGGTSSSNAVSDTAAQYIENKGSDTIV
                     NLALAWAEKYQADHVDVRISVTGGGSGTGIAALINNTVGIANASRKIKDEEIAEAQAK
                     GVTPVEHIIARDAIAVIVNPENPVNELTLQQISDIYSGKITNWSEVGGDDRPIVKLSR
                     ETNSGTHVYFLETVLRLGNKEDKTLFSTDTLLLPSSEGIISEVRDNPNAIGYDGLGYV
                     PDDLKMIAIAKEAGAPYVLPSIETVNDKSYAIARDLYMYTNGEPSGVIKEYLEWILSA
                     EAQQIVADLGFVPVK"
     gene            6278..7174
                     /gene="pstC"
                     /locus_tag="PL000_16790"
     CDS             6278..7174
                     /gene="pstC"
                     /locus_tag="PL000_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC90589.1"
                     /translation="MEKSLSLREFIITRLIQASGYSAILFVALIFFFLLREGLPTLSE
                     VESSALLNVRWYPIENYFGILPLISGSLIVTLGAALIAVPFGIGTAVYISEIAPRWVR
                     EILKPLVELLGGLPSVVLGFLGILIVSPFLRVFLDLPTGLTAFTGSLLLGGIAVPTVV
                     SVAEDALDAVPRAYREGAWALGATKWQTIWRVTLPAARSGVLTAIMLGVGRAIGETMT
                     VMMVTGNAPVLASSLGSLFSPVRTMTATIAAEMGEVANGSTHYHVLFFIGMVLFLISL
                     VVNVIASAVVFRAKKRAERILS"
     gene            7250..8113
                     /gene="pstA"
                     /locus_tag="PL000_16795"
     CDS             7250..8113
                     /gene="pstA"
                     /locus_tag="PL000_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC90590.1"
                     /translation="MMKLLARNRHTVQKLGFGLITLMAVVTVIPIVGTIVFILFKGGS
                     AISLEFLSGFPRDGMREGGILPAIIGTLYLTVGTAVFSVPLGIAAAIYLAEYAKDTAL
                     TRMIRLAIINLAGIPSVVYGLFGLGLFVLFLKFGTSILAASLTLSIMTLPVIISTAEE
                     SLRSVPQAFRTVSISLGATRWQTIRRIVLKEALPGILTGVILGLERAAGETAPILFTG
                     AAFFLPRLPGSPFDATMALPYHLFVISTQVPEMPIQIQYGTALVLLVFVLTMNVIATV
                     IRSRARAKRQW"
     gene            8141..8899
                     /gene="pstB"
                     /locus_tag="PL000_16800"
     CDS             8141..8899
                     /gene="pstB"
                     /locus_tag="PL000_16800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC90591.1"
                     /translation="MNNKILIENLSLQYSDGTESLRGVNMEIRQNAVTVLFGPAGGGK
                     STLLRCLNRLNDLTEVRASSGQILIDGENILDPKVDVVALRRKVGMVFARPVPLPMSI
                     RENVVYGLEMAGEKRKSKLDEAVERSLKLAAIWDEVKDRLNDPANALSGGQQQRVCLA
                     RVLALQPEVVLLDEPTSGLDPISTGKVEAALQELKKEYTIILVPHSVQQAARTADHAA
                     FFLQGELIEYDEGKKMFTTPKQKKTEDYIMGRFG"
     gene            complement(8974..10632)
                     /locus_tag="PL000_16805"
     CDS             complement(8974..10632)
                     /locus_tag="PL000_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90592.1"
                     /translation="MNNELPAKSIQVLTQQWGLILALLTFIGWGSLLLYAHLKRNTGF
                     QFTDLELAALSLGGWPLPALLISFPVLFLSAFLPAGVLIGITAVILLTSGGIALRAIW
                     KGNSPWALVPVSIFLVLAFLRLGLVADVIMPMYFDSAEHYRIIRELLDSAWPTASYYH
                     LGYHVIIAAFVRISAADPASVMLIFGQLILAALPLPIFFLLYRATFSKSAALLGVIFA
                     AFGWFMPAHAVNWGKYPALLSLLLLQFTLGILLIKKNWMPAVAALAATVIHTRSVILL
                     ALFGAAWILANLWEGQPHPRRRLILAVVATMLGMLTLIIYRNPILGPILEPYATLITL
                     LSALLAASVFKAFSHPIMVSLLIILLILTAIFIPVSSSLTLLDRPLAEMILFLPLAFI
                     GGLGGARPLKFIAPALAALIILNAWMNISFGPSKCCQLVSRDDATALDWVGRHYKKDS
                     VIAIAGVNLNINAFGRPMRGAGIDAGIWINPLTGIQTVPFPNSTNFASPEAHDQLCSN
                     GITHIYVGARPQSFASDFAELVPEWYETTFSLPKAHIVKVLNCK"
     gene            complement(10775..11380)
                     /locus_tag="PL000_16810"
     CDS             complement(10775..11380)
                     /locus_tag="PL000_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90593.1"
                     /translation="MTRKRLYKILGLFSAAVLLALATAFPLIRGLLSADSKPNPEQNI
                     FEMPLCDLQPGGLCLVTFGAISSAEMAIVIQTPGGEYPAFYLRATHQGVENTYPCEAG
                     SLPNQVICVGARTPLGEPLEIKVFTNESDLLIASGKFNVSAMATTLLGSNSGFSVMGK
                     MATPTPSPSPSPSPTVQVIFTPTSAPIATMTSTPDVGYPNP"
     gene            complement(11383..12084)
                     /locus_tag="PL000_16815"
     CDS             complement(11383..12084)
                     /locus_tag="PL000_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNC90594.1"
                     /translation="MTGTILNIATILLGGVIGLIFGSRIPERFKGTVIAGMGLFTAAM
                     GLQMFLKSENQLVVLGALIIGALTGEWWKLEDRLQALGQTLEQRFSQDSETGAGSKFV
                     RGFMVSSLLFCIGPMAILGSIQDGLTGDYQLLAVKSMLDGFAAIAFSSTLGVGVLFSS
                     VVVFVYQGGISLLAGQLNSIVTDPMMNELTATGGVLLIGVGISNLLELKKIRVGNFLP
                     SLIAAPLIVWILGLF"
     gene            complement(12270..12812)
                     /locus_tag="PL000_16820"
     CDS             complement(12270..12812)
                     /locus_tag="PL000_16820"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC90595.1"
                     /translation="MTFYTAKGDDGTTGLLGEGRVPKYHARMEAVGALDEASAALGLA
                     RAQSAAPQTASILLEAQRDLYKLMAEVAATPENAERFRFINEARVKWLEEQANELSAV
                     VEMPKEFILPGDTLGGAALSMARAIIRRAERRVVGLFDEEEVGNPDLQRYLNRLSSLC
                     FVLELLENQNAGKQTTLAKA"
     gene            12879..13505
                     /locus_tag="PL000_16825"
     CDS             12879..13505
                     /locus_tag="PL000_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90596.1"
                     /translation="MLIPIHQEMTREALEGRVSPRALEIIIAANCKQDALSGQIGHDE
                     FHYDNNAIDEGHRYVIEQRGFVIASLLSPGVLSAWIAFGRLTHTVQDFYSHTNYVTMW
                     LDSHPSGTPPTPPEIDPVQKDLIESPSLHSGKLYMPMDWLYFVPFLRKLSLSLLPADS
                     HAKMNLDSPVQGAKFAYARAAAVKRTIYEFDLLEKLLTPEMFSKFVDM"
BASE COUNT         3153 a         3442 c         3677 g         3315 t
ORIGIN      
        1 acctgaacaa cgtcaacgac aacattctcg atgtgggtta cttccccgct cctgccgatg
       61 ctcttcaagc ttcaatggat gcctggatga ccgccaccgg gaactaaaag aaaaatatgc
      121 cgacgggctg gtctctgaat tgaggccagc ccatttatgc tgtaatcaca gatccacaca
      181 ttgctccgtt aagtggatct ggactggaaa ccatagagcg aacatgaaac aggaaaatat
      241 gaccgcggaa aataaaaact tccgcccgct caatttacaa aagaaactcc gccccgctga
      301 aagcgccatt caagtgttgc tttttcttgc gggagttttg tctatttttg tgaccattgc
      361 cattgtgtat gaattgggca aggaagccat gctatttttc accgaccccg atgtaacgct
      421 ggcaaaattc ctgggcacca caaaatggca gccaggtatc ggacaatacg gtatttgggc
      481 cctggtctca gccaccctgg taacaagttt tatcgccatc tccatctccc tcccgcttgg
      541 gctggctgcc gccatctacc tcagtgagta tgcctccttt aagtcacgtt ccattctgaa
      601 accaattctt gaagtgcttg ccggtatccc aacggttgtt tacgggtatt ttgccttgtt
      661 gttcgtcacc ccggtattga aatccatgat cggcgatcag cttcaagttt ataacatgct
      721 gtcggcgggg atcgtgatgg gcatcatgat cctgccgctg atctcttcga tgagtgagga
      781 tgccttgagc gccgtgcctc gttcactgcg cgaagccgct tatgcaatgg gtgccaccaa
      841 agtggagacc agcctgcagg tggttgtgcc tgcggcgctt tcggggatcg gcgccgcgct
      901 gatcgtgggc atttcacgcg ctgtcgggga gacgatgatc gttgctgtgg catcaggctc
      961 aggcccgaat ttttcgttca atccactgga tgcggctgaa accatgaccg gtcatatcgc
     1021 ccgcatcagc ggcggtgacc tttcctataa cagcattgat tacaccagcc tctttgccat
     1081 tgcgatgatg ttgtttctgt tgacccttgt gttgaatctg atcagtcaat gggtggtgag
     1141 ccgcttcagg gaaagatatg agtaatcaca tgaatatgaa tcaacattac ccgcaagacc
     1201 gggaatttcg gcgcatgctc gatgcgcgtt atcgtgtggc gaccctttgg caggccctgt
     1261 tcctctcggc gttgttgatc gcgatcgtca gtttgatggc gttgatttac aacgtggtgg
     1321 atggcgcctt cggctacatt gcctacgaat ataaaaagga tcccgctcaa ttcacctcca
     1381 ccccgatcgc gaatctgact caggaggaac tggccgccat tctcaaagag aatctttctt
     1441 cgggtgcata caagaaatta aacaatgaac agcctgtagc cgagcgttca caagcggatc
     1501 tgttcaacct gttcatggaa cgtcttgtgc agttggatac caaagccacc tggagtatga
     1561 ccgattcgtt gatgaatggc gaggatatcc gtgcagaggt tgcagagaaa taccccaagg
     1621 cacagcttga attccgctca tggctcaccc cgcagttttt ggtcaccccc atgtcatcga
     1681 aggctgaatt cgcaggggtg cggaccgctg tgttggggtc gttgtggctg gtgggaattg
     1741 cgatcctgtt cgcactgccg atcggcaccg gcgcggcgat ctatttgcag gaatatgcgc
     1801 cgaagaattg gttcaccgcc attattcaga ccaacatcaa taacctggcg ggtgtgccca
     1861 gcatcgtcta tggcatgttg gggctggcca tctttgtccg tgtgttggag cctttgacca
     1921 gtggggcctt gtttggcgtc acagactcga atggtcgcac gatcctttcc gcggggctga
     1981 cgatgggtgt gcttgttctt ccgttgatca tcatcaacgc acaggaggcg atcaaggctg
     2041 tgccggattc gctcaggcag gcggcgtacg gcgtcggtgc cacccgctgg cagaccgttt
     2101 ggtaccatgt gctgccgaat tcattgcccg gtattctgac cggcagcatt cttgcgcttt
     2161 cgcgtgcgct tggcgagacc gcaccgttga tcgtggtggg ggcctccact tttatcagcc
     2221 tcgacccatc cgggccattt tcaaaattca cggccctgcc cattcaaatc tatcaatgga
     2281 ccacccgcgc ccagtctgaa ttccatgcca ttgcggcggg tgcgatcatt gtcctgctcg
     2341 ttcttctttt gtcgctgaat gcaaccgcgg ttttgctgcg taatcgcttt cagagacgct
     2401 ggtaatctgg aatttgtgga tataaaccca tggaaaaaaa ttacgcaatc gaatccaaca
     2461 agctctccat tcactacggc aatttcaccg cagtaaggga tgtaagcatt aaaatcgagc
     2521 cgcagaagat cactgcgatc atcgggcctt cagggtgtgg aaaatccacg ctgctgcgtt
     2581 cgttgaaccg catgaacgat tttgtgcagg gctgtcatgt caccggcgaa ttgttgctgc
     2641 agggacaaaa cctctatggt aaaaatattg accccgtgga agtgcgccgc aagatcggca
     2701 tggtctttca aaagccaaac ccgttcccga aatccattta tgaaaacatt gcctggggcg
     2761 cacgcattaa taacttcaag ggaaaaatgg atgaccttgt ggaacagtcc ctgcgcagtg
     2821 ccgccttgtg ggatgaggtg aaggataaac ttcaacaaag cgcatatgcc ctctccggcg
     2881 gtcagcagca gcggttatgc attgcccgca ccatcgccat tcagcctgag atcattctca
     2941 tggatgaacc tgcttccgcg ctcgacccca tctccacgct ccgcatcgaa gaactgatgc
     3001 aggagttgaa gaagaactac accatcatca tcgttaccca caacatgcag caggcggcac
     3061 gtgcttctga tttcaccgcc atgatgatga tcgatgaaca gcgcgcaggc ggtgtgatcg
     3121 aatttgatga aaccaaaaag atattcaccc gtccgcagga taaacgcacc gaagattatg
     3181 tgacgggacg ctttgggtaa cacaccctcg caatgaaatc atggagaaaa cgatgaacaa
     3241 accaaaggtc atttttcttt gcactggcaa ctccgcacgc agccagatgg ctgaagggtt
     3301 gctgcgtgcc ctggcagggg agcacttcga agtgttcagc gcaggcaccg agccaaaggg
     3361 caggatcctg cccgaggtcc aggcggtgat gcgcgaggtg ggcatccata tggaggggca
     3421 attttccaaa tcggtcatgg aatatcttgg acgggctgtt tttgcgcacg tcgtcacagt
     3481 gtgtgccgat gccgaagaga attgtcctgc tgtcttcctc aacatgggga cacatgaaca
     3541 ctggcctttt gatgatcctg ccaaattcgc cggggatgac gaacagcgca tcacccatac
     3601 ccgacgcgtg cgcgaccaaa ttgaggagcg tctgcgcctt tggctgaccg ggcagggcat
     3661 taccccagcg gactatcgtc acacgtagtt gatggtagta aaataagaaa aaccatcaga
     3721 ggtcttatga tcagaaaaac atttgagagt gaaattcaac tggtgaaaga tgaagtgctt
     3781 ttgctgggca gcatggtgga acatgccatc atcggctcgg tggaagcgtt gaagaagcgc
     3841 gacatcaagg cttctgagaa attaatcgcc gaagacaagg aaatcaacaa aaagcggttt
     3901 gcgatcgaaa atcagttgat gatcctcatt gcgacccagc agccaatggc acatgacctg
     3961 cgcctgctgg cctccaccat ggaaatcatt tcagagttgg agcgcatggg cgattacgcc
     4021 aaaggcatcg ccaacatcaa cattcgcatg ggtgaggaaa ccctgctcaa gccgttgatc
     4081 gatattccgc gcatggctca aaaaggcgcc gatatgctcc atcgtgcgtt gaccgctttt
     4141 gtcaacgaag atgtgcaggc tgccaatgcc atccctgtgg aagatgatga ggtggatgcg
     4201 ctctacaacc agatctatcg cgagttgatg atgttcgtga tccaggatcc aaagaccatt
     4261 gagcgcgcga attggttgtt gtgggtggcc cataatctgg aacgtgtggc cgaccgtgtg
     4321 accaatatct gcgagcgtac cgtatttatc gccacaggtg aatttggcga gatcaaatcc
     4381 agtgatgatg aattctggaa aggtcagaag ggttcgtaaa aagagttggc aaaacaaacc
     4441 cgccccgagt atttcggggc gggtttgttt tttcattgtt aatttctatc ccatcctgcc
     4501 tgtgatgtag gcttcggtca gttcctcttt tggaacggtg aacatctgat tggtcggtgc
     4561 gaactcgacc aattctccca gccagaagaa gccggtgtgg tctgaaacgc gggcggcttg
     4621 ctgcatgttg tgggtgacga tgacgatggt gtagtctttt ttcaaatcgg cgatcagttc
     4681 ttccacgcgc agggtggcga tggggtcaag cgcagaagtg gactcatcca tgaggatgac
     4741 ttcaggttga acggcgatca cgcgggcaat gcacaaacgc tgctgctggc cgagggatag
     4801 gctcatggcg tcggatttca aattgtcttt cacttcatcc cacaatgcgg cggcggtgag
     4861 gcttttttcc accacttcgt tcaggtttac atccatgccc atcacacgtg gaccaaaagc
     4921 cacgttatcg aaaatggact gtggaaatgg attcggtttt tgaaagacca tgcccacccg
     4981 ttgacgcagg gtgaccacat ccatggcggg ggcgtagata tcctcgcctt tgagcaggat
     5041 cttcccgtcc acatgtgtgc cggggatggt gtcgttcatg cgattgaggc agcgtaaaaa
     5101 cgttgatttg ccgcatcctg acgggccgat gagtgcggtc actttttgcg gctgaatctc
     5161 aaggttgata ttcgacagcg tctttttggc gccgctgtag taaaaatcca tgttttggat
     5221 ggtgaaggcg ggttgggttg cgtccatgaa atgattttat caaagaaagg gatgaaggat
     5281 gagggcgaat gataaaggga gatttgattt tttggatcag ggtcagtttt cggcgcgttt
     5341 gtttcgattt gtctacttgt ttctgagcct gccaacgact ataatccatc ccaatgaaac
     5401 gcgctgcttt tttcttttta cttgtctctt tcctgcttgt ttcctgcgga gggacatctt
     5461 cttccaatgc tgtttctgat acagcggctc aatacattga aaacaagggt tccgatacca
     5521 tcgtcaatct ggcgttggcc tgggctgaga aatatcaggc ggaccatgtt gatgtgcgca
     5581 tttcggtgac gggcggcggt tcagggacgg gaattgcggc tctcatcaac aacacagtgg
     5641 ggatcgccaa tgcttcacgc aagatcaagg atgaggagat cgccgaggca caagcgaaag
     5701 gtgtgacgcc tgttgaacat atcatcgcgc gcgatgccat tgctgtgatc gtgaatcctg
     5761 aaaaccctgt caacgagttg accttgcaac agatctccga tatttatagc ggcaagatca
     5821 ccaattggag cgaggtgggc ggtgatgatc gtccgatcgt aaaactttcg cgtgaaacca
     5881 attcgggtac gcatgtatat tttttggaga ccgtgctgcg tttgggaaac aaggaagata
     5941 agaccctgtt ttccacagat acgttgctgc tgccgtcttc ggagggcatc atctctgaag
     6001 tgcgtgataa cccgaatgcc atcggctatg atgggttggg gtatgtgccc gatgatctga
     6061 aaatgatcgc cattgcaaaa gaagcgggcg caccctatgt gctgccttcg atcgaaactg
     6121 tgaacgacaa aagttatgcg atcgcgcgcg atctttacat gtataccaat ggtgaaccca
     6181 gcggcgtcat caaagagtat cttgagtgga tcctttccgc cgaggctcag cagattgtgg
     6241 ccgatcttgg gtttgtgcct gtgaaataga gaccgccatg gaaaaatccc tgtcccttcg
     6301 tgagttcatc atcacccgcc tgatccaggc ttcgggttat tctgccattc tgttcgtggc
     6361 gttgatcttc ttcttccttt tgcgcgaagg attgcccacc ttgagcgaag tggaatcttc
     6421 agccctgctc aatgtgcgct ggtacccgat cgaaaattat ttcggcattc ttccgttgat
     6481 cagcggttcg ctcatcgtta cactgggcgc ggcattgatc gccgttccgt ttggaattgg
     6541 taccgccgtg tatatttccg agatcgctcc gcgctgggtg cgtgaaattc tcaaaccctt
     6601 ggtggagttg ctgggcggac ttccttctgt ggtgttgggc tttcttggaa ttttgatcgt
     6661 ctctccattt ctgcgtgtct ttctggacct gccaacgggt ctgaccgctt ttacaggatc
     6721 cctgctgttg ggtggcatcg ccgtgccgac agtagtctct gtagccgaag atgcgctgga
     6781 cgcagttccg cgtgcgtatc gtgaaggtgc ctgggctttg ggtgccacca aatggcagac
     6841 catctggcgg gtgaccctgc ctgccgcccg ttcgggtgta ctcactgcca tcatgctcgg
     6901 ggttgggcgc gccatcggcg aaaccatgac cgtgatgatg gtgacaggga atgcgcctgt
     6961 gctggcttcc agtttgggca gcctcttttc cccggtccgc accatgaccg cgaccattgc
     7021 cgctgaaatg ggtgaggtgg caaatggcag cacacattat catgtgctgt tcttcattgg
     7081 catggtgctc ttcctgatct cactggtcgt caatgtcatc gcttcggcgg tggtcttccg
     7141 tgccaagaaa agagcggaga gaatcctttc gtagagtttt tgctcccttt cttttttatc
     7201 gaatggcgta aaggaaagag agaagaagaa agaagccctg ttttcaatta tgatgaaatt
     7261 acttgcacgc aatcgtcata cggttcaaaa gctcggtttc ggcctcatca ctttgatggc
     7321 ggtcgtcacg gtcattccca ttgtcggtac gatcgttttc atccttttca agggcggttc
     7381 ggccatttcg ctcgaattcc tttcgggttt cccccgcgat ggaatgcgcg aaggcggaat
     7441 ccttcccgcg atcatcggta cgttgtacct gacggtgggc accgctgttt tctccgtgcc
     7501 cttgggcatt gccgcggcca tttacctggc agaatatgcc aaagatacgg cgttgactcg
     7561 catgatccgt ctcgcgatca ttaatctggc agggattcct tccgtggtgt atggcttgtt
     7621 tggcctgggt ctgttcgtgc tttttctgaa attcggcaca tccatcctcg ccgcttccct
     7681 gaccctttcc attatgaccc tgcctgtcat catcagcacc gcagaagagt cgctgcgttc
     7741 cgtgccgcag gcattccgca cggtgagcat ttctcttgga gccacccgtt ggcagaccat
     7801 ccgccgaatt gtgttaaagg aggccctgcc cggtattctc actggggtca tcctggggtt
     7861 ggaacgggct gccggtgaaa cggcgccgat cctttttacg ggtgcagcct tctttttgcc
     7921 gcgtttgccc ggttccccat tcgatgcgac catggcattg ccgtatcatc tatttgtgat
     7981 ttccacacag gtccctgaaa tgccgatcca gatccagtat ggtacggcgc tcgtcctgct
     8041 ggtttttgtg ctgaccatga atgtcatcgc cacggtgatc cgttcacggg cacgtgcgaa
     8101 acgccagtgg taaccatcca ttgcaagttg aatatcgcaa atgaacaata aaatccttat
     8161 cgaaaatctt tccctgcaat attccgacgg caccgagtca ctgcgcggtg tgaacatgga
     8221 aattcgccag aatgcggtga ccgttttgtt cggcccggca ggtggcggaa aatccacgct
     8281 gctgcgctgt ctcaaccgct taaatgacct gaccgaagtg cgcgccagtt caggtcagat
     8341 tttgatcgat ggtgaaaaca tccttgaccc gaaggttgat gttgtggccc tgcgccgcaa
     8401 ggtggggatg gtgtttgccc gtcccgtacc actgccaatg agcatccgcg agaatgtggt
     8461 ttatggattg gaaatggcgg gtgaaaagcg aaaatccaag ctggatgagg ctgtggagcg
     8521 cagtttgaaa cttgccgcga tctgggatga agtgaaagac cgattgaatg accctgcgaa
     8581 tgcgctttca ggcgggcagc agcaacgtgt ctgccttgcg cgtgtgctgg ccctgcaacc
     8641 tgaagtcgtt ttgctcgatg aacccacttc gggtctcgac ccgatctcca cgggcaaggt
     8701 ggaagccgcc ttgcaggaat tgaaaaagga atacacgatc atccttgtgc cgcattccgt
     8761 tcagcaggcg gcgcggaccg ccgaccatgc ggctttcttt ttgcagggtg agttgatcga
     8821 atatgacgaa ggcaaaaaga tgttcaccac gccgaagcaa aagaaaaccg aagattacat
     8881 catggggcgt tttggataag cgcagaagta aaagaccgtc gacaaccgtc gacggtcttt
     8941 tttgaattcc tctgctaaag gcggcaagtt gggttattta cagttaagca ctttgacaat
     9001 atgtgctttc ggaagcgaga aggtggtttc gtaccattct ggaaccaact cggcaaaatc
     9061 ggaggcaaaa ctctgcggcc ttgcgccgac ataaatgtgg gtgatcccgt tgctgcaaag
     9121 ctggtcatga gcttctggtg aagcaaagtt ggtggagttg gggaagggga cggtttggat
     9181 ccctgtcagc gggttaatcc aaatgcccgc atcaatgcct gcaccgcgca tgggtctgcc
     9241 aaaagcgtta atattcaggt tgacccccgc aatggcgatg accgaatcct ttttgtaatg
     9301 cctgccgacc caatcaagcg cggttgcatc gtcacggctt accagttgac agcatttcga
     9361 aggtccgaag ctgatgttca tccatgcatt caggatgatc aatgcagcca gcgcaggcgc
     9421 aataaatttc agcggacgcg caccgcccaa cccgccaatg aaggcaagcg gtagaaagag
     9481 gatcatttcg gccaatggcc tgtcaagcag ggtcaacgat gaagagacgg ggataaaaat
     9541 ggctgtaagg ataagcagga tgatgagcag agagaccatg atcgggtgag agaaggcctt
     9601 gaaaacggaa gcagccaaca gcgcggaaag aagcgtgatg agcgtcgcat acggttccaa
     9661 aatcggaccg agaatcgggt tccggtaaat gatcagagtc agcattccca gcatggtggc
     9721 gaccaccgcc aaaatgaggc ggcgccttgg gtggggttga ccttcccaca ggttggcaag
     9781 aatccaggcc gcgccaaaca gggcaagcag aatcaccgaa cgggtgtgga tgaccgtggc
     9841 ggcaagtgcc gccacagcgg gcatccaatt ttttttgatg agaaggattc ccaatgtaaa
     9901 ttgaagcaag agcaggctaa gcagggcggg atacttcccc cagttgaccg catgtgccgg
     9961 catgaaccag ccgaaagcgg cgaagatcac accgaggagt gccgccgatt tcgagaaggt
    10021 ggcgcggtac agcaaaaaga agatcggcag gggcagtgct gcaagaatca attgcccgaa
    10081 gatgagcatg acactggcag ggtcagcggc gctgatgcgc acaaaggctg cgatgatgac
    10141 gtgatagcca aggtgataat aagaagcggt gggccaggcg gaatcgagta attcacgaat
    10201 gatgcggtaa tgttcggcag agtcgaaata cattggcatg atcacatcgg ccacaagacc
    10261 cagccgcaga aaagccagca cgagaaaaat ggaaacaggg accaacgccc agggcgaatt
    10321 ccccttccaa atggcgcgaa gggcaattcc tccgctggta agaaggatga ccgccgtaat
    10381 gccgatgaga acgccagcag gaagaaacgc gcttagaaac agcactggaa aggaaatcaa
    10441 caaggcaggc aggggccagc cgcccaagga tagggcggct aactcaagat cggtgaactg
    10501 gaagcctgtg ttccgtttta aatgagcgta gagcaacagg gagccccagc ctatgaaggt
    10561 cagcaatgca aggataagcc cccattgttg agtgagcact tgaatgcttt tggctggcag
    10621 ttcgttgttc atgagaattt agcttttttg aaccgtaatg actgggggaa aacaggggtt
    10681 gaagtttttc taccgcacat tcgcccgttt ttactgtgaa gatcgctgcg ttttaacttt
    10741 tttctttgcg atctttgtag ttcaaatttg tcatttatgg gttggggtaa ccaacgtccg
    10801 gtgtggaggt catagtggct atgggagcgg aagtgggtgt gaagatgact tgcacggtgg
    10861 gcgatgggga aggcgaggga gatggggtgg gggtggccat tttgcccatg acactgaatc
    10921 cgctgttgct ccctaaaagg gtggtcgcca tcgcagagac attgaatttt ccgctggcga
    10981 tcagcagatc gctttcgttg gtaaatacct tgatctcaag cggttctcca agaggggtgc
    11041 gcgcacccac acagatgacc tgattgggca gggaccccgc ctcgcacgga taggtgtttt
    11101 ccacaccttg atgtgtggca cgcaggtaaa aggcggggta ttctccaccg ggtgtttgga
    11161 tgacaatggc catttctgct gaggagatgg cgccaaaagt gacaaggcat agcccgccgg
    11221 gttgcagatc acacaagggc atttcgaaga tgttttgctc agggttgggt tttgaatcgg
    11281 cggagagcag ccctctgata agcggaaaag cagttgccag cgcaagcaga accgcagcgc
    11341 tgaatagtcc taaaattttg tagagtcttt ttcgtgtcat ttctaaaata aacccaggat
    11401 ccaaacgatc aatggagccg caattaatga aggcaggaaa ttcccaacgc gaatcttttt
    11461 gagttccagc aggttgctga tgcccacccc gatcaaaagc accccgccgg tggcggtcaa
    11521 ttcattcatc atcgggtcgg tgacgatgga attaagctgc ccggcgagca gactgattcc
    11581 gccctggtac acgaacacga cgaccgatga gaataataca cccactccca acgtggatga
    11641 aaatgcgatt gcggcgaaac catccagcat ggattttaca gccagcagtt ggtaatcgcc
    11701 tgtcaaacca tcctgaatgg agccaaggat ggccatcggg ccgatgcaaa ataaaagcga
    11761 agataccatg aatccacgca cgaatttgga gcccgcacct gtttctgaat cttgcgagaa
    11821 acgctgttcg agggtttgcc ccagggcttg cagcctgtct tcaagtttcc accattcgcc
    11881 ggtcaatgcg ccgatgatga gtgcgccgag caccaccaac tggttttcac ttttcaagaa
    11941 catttgcagt cccattgccg cggtgaacaa tcccatgccg gcaatgacgg tgcctttgaa
    12001 ccgctccgga atacgtgaac cgaagatcaa accaataacc ccgcccagaa gaatggttgc
    12061 aatattgagt atcgtacctg tcatgttttc cttgtgtgct cactttgcgc acttgagttg
    12121 atacggcgcg aggtttgtct aaactttgat cgtggtctgc cgtctgtggt caatgccttc
    12181 atcagttttc aaagcgctgc gtaaggtgag tttaattaaa tgtagagtaa tacagggcgc
    12241 cattctttat ccaaggcact ctgttgtttt tatgcttttg ccagtgtagt ttgtttgccc
    12301 gcgttttggt tttcaagcaa ttcaagcaca aaacacagcg atgaaagccg attgagataa
    12361 cgctgcagat cggggttgcc tacttcctct tcatcgaaca gccccaccac acggcgttca
    12421 gcacggcgaa tgatggcacg cgccattgaa agagccgccc cgccaagcgt gtcacctggc
    12481 aaaatgaact cttttggcat ttcaacgacc gcacttaatt cattggcctg ttcttcaagc
    12541 catttcacgc gggcttcatt gatgaagcgg aaacgctctg cgttttccgg tgtggccgcc
    12601 acctcagcca tcagtttgta cagatcgcgc tgtgcttcaa gcaaaatcga agccgtttgc
    12661 ggtgccgcgg attgtgcacg tgccagcccc agcgcagccg aggcttcatc caatgcgccg
    12721 accgcttcca tgcgggcatg atatttgggg acacggccct cgcccagcag gcctgttgtg
    12781 ccgtcatcgc cctttgcagt ataaaaggtc atgcaggcat tataaccagt ttgaagcccc
    12841 gcacagattc tccagcccag actgggtata attttgaaat gcttatcccc attcatcagg
    12901 aaatgacccg cgaagccctt gagggccgtg tcagtccgcg tgcgcttgag atcatcatcg
    12961 cggccaattg caaacaagat gccctcagcg gtcagatcgg ccatgatgaa ttccattatg
    13021 ataacaacgc cattgacgaa ggtcatcggt acgtcatcga acagcgcgga tttgtcatcg
    13081 catccctgct ttcgcctggt gtcctctctg cctggatcgc ttttggcaga ttgacccaca
    13141 ccgtacagga tttctactcc cataccaact acgtcaccat gtggcttgat tcacacccca
    13201 gcggcacgcc tcccacaccg cccgaaattg atcccgttca aaaggacctg atcgaaagcc
    13261 caagcctgca ctccggcaaa ctctacatgc ccatggactg gctgtacttt gtaccttttc
    13321 tccgcaaatt atcactgtcc ctgctcccag cggattctca tgcgaagatg aatcttgatt
    13381 cgcctgttca gggggcaaaa tttgcctacg ctcgtgctgc tgcggtcaaa cgtaccatct
    13441 acgaattcga tctattggaa aaattgctca cccctgaaat gttctccaaa tttgtggata
    13501 tgtaaaaagc ctactcttta ttttctaaac caatggtcat gccctgaccg tcgtcctatt
    13561 tacagcggtt gacgagattt tgtttcc
//
LOCUS       DAOQLC010000116        13404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1720_length_13404_cov_24.019552, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000116 DAOQLC010000000
VERSION     DAOQLC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1720_length_13404_cov_24.019552"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="PL000_16830"
     CDS             complement(<1..1631)
                     /locus_tag="PL000_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNC90597.1"
                     /translation="MIQLQLSNITLVLGAKRIFENLNWEIQNGQKIGLIGANGAGKSS
                     LFKLIEGEHSPELGGSITRARLITTGYLSQHPELDLTLTAFEAALAGNPRVAEVQADL
                     ERVEASLGDAEVYGNERRLQQALELQHKLIEEYHALGGDSYPARVRSMLLGLGLAESE
                     LNKSLSILSGGQKKLVGLARLLLVRPDILLLDEPDNHLDLPGKMFLEKLIREYDGTVV
                     LISHDRYLLDAAVTHIAELEDGKLTVFEGDYSSFIADKETRMARQEELFRAQQHEITR
                     MQIAIKRFAIWGKIYDNEKFAAKAKTMQKRLDKMDKIEKPVLDRKRMELRLNGWRGSN
                     KVLELAGVSKSFGSKQVFANLNETIWHGERVGLIGPNGAGKSVLLRMILGKETPDVGE
                     IKIGPSISIGHYAQEHETLDFNQTVVEAVRYAGEMSESRATAFLLRYLFTYKQVSQKI
                     GELSGGERSRLQLALVVLSGANFLLLDEPTNNLDIASAEVLEQALEDFEGTVLVISHD
                     RYFLDRTVERLLVIEDGQLGGYQGGYSDYLGAKGK"
     gene            1830..2651
                     /locus_tag="PL000_16835"
     CDS             1830..2651
                     /locus_tag="PL000_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC90598.1"
                     /translation="MPDQLNTLSQSSLQDYYDCPRRFELRHLQHLVYPAVETEPALEN
                     EKHQQEGEYFHRLVQQHLIGIPAEQVGRLANTPNLQRWWENYLSAKDLASLRNHSKLY
                     AETTLSAPLGNLRLVAKYDLIAVSSEQVLIYDWKTYRKRPKNDWLSIRWQTRVYRALL
                     VQAGAHLNGGKPFAPEQIEMIYWFADFPNEPARFTYKAEQFKRDWDALVKISDEIKTA
                     SDFPKTDDASKCSYCPYRSYCNRGIRAGDAADAELETEAEELFDINFEQIGEIAF"
     gene            2820..2891
                     /locus_tag="PL000_16840"
     tRNA            2820..2891
                     /locus_tag="PL000_16840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2853..2855,aa:Arg,seq:ccg)
     gene            2959..4041
                     /locus_tag="PL000_16845"
     CDS             2959..4041
                     /locus_tag="PL000_16845"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC90599.1"
                     /translation="MALRNILFIIFVMLVCTGCGVKLTGEGASTAAPDFVTSTLPPSA
                     PPLATQTPIAQQSIAPTAMATHLPAEGITNTQVNIRAATSTASTSFGVIGAFETVQVL
                     GKDASGSWYQIMYAGSPNGNGWVRAEYVQVNDAGKILPVEGLTGNGGLVLEAVNLRSG
                     PGSDFESLGVLSSKDAVLVTGKDPSGAWVQVEVTGAENLKGWVTVKFLQVENLEAIPV
                     IGEIASPTSLPAEEVSTAIPPASQDGDSLQSPLTVVRFSSTESRAMQIQGNVSAPAGD
                     TEDWIQFSTGGNGVVIEALCSSDTLTIELWFDGQRTDGFSQICGEKQFVTVEPNGKYV
                     LRILETSTNQFRNTDFILTIEDIHQP"
     gene            4038..4898
                     /locus_tag="PL000_16850"
     CDS             4038..4898
                     /locus_tag="PL000_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90600.1"
                     /translation="MKSLPSIDTMGSRPDIIRNKRIFGMIYGIMAGLAFAGSSWGWDG
                     YTLSQFHGYFPWNVLVTGALLCAIVGGFIGWLTARMESSLAGVLFWLGGSFFFAWLTV
                     ALPLQISPIIATKMDPQLAGLLDYSSNIEFTSRFGVALAWILPFTVIVGIAQIPVTEP
                     AVFSTSFFGKIGPLLFGMLVISIGGVITDSLINAQFRSAIIALDKTAQFVLDNRGNEN
                     VDPLVAREIHARAFGTVEESMTDTRYLFVGSFDETLGDINILVKFEKAWVTCEVLYNQ
                     PITCKPVQSE"
     gene            4914..6788
                     /locus_tag="PL000_16855"
     CDS             4914..6788
                     /locus_tag="PL000_16855"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC90601.1"
                     /translation="MEKSPKTFNLRFVLLLGTVIVLLLIQFSALLPNVTTPVERIEYS
                     TRDVLMRLRGAQDPSQNIVIVEIDDFSFNWTGYQWPWPRSYLAGIVDQINKGGGKVVG
                     VDIFLSEPDEDTANDQAFATALANAPAAVGVVQIFESPVDTFSISTLLQAQPVYQSAL
                     DANGITAFTRDEDAIVRSVQAYDFYKDKVYYHWAFEVARLYLDVEPPTQPTDTSIQFN
                     GQSVPLRAGHVLVNFAGPSRTYTTYRAADVHDGITLEENPDAFRGKIVLIGATTVTLQ
                     DMYPTPFSTQIPTAGVEIVASTIDTLITGQYLTEAPPWLALALTILAAVIASLISRSN
                     RPTLTIVLLVLVMAGYAVGGVVLFLRQRYILPFVVPQVMLFLGIVLPTLEQAVSQELE
                     KRRVRNLFSRFISPEMVDQMMSTQDLNSLNKRSNISIIFSDIRGFTTLSEKLAPEDVV
                     ALLNPYLEAMSKVIYKHGGTVDKYEGDAIIAFFGEPVPFKDHAIRALRASLDMRKALD
                     ELKAQWEKEGRPTQIEMGIGINSGDVFVGLLGSAQRINYTVIGDNANLASRLQDLTKT
                     YAWPILISESTYQQVKDEFDAEFADAVTVKGKTKPVNVYKVLGYKGAPPAEQLQSWQK
                     "
     gene            6883..7530
                     /locus_tag="PL000_16860"
     CDS             6883..7530
                     /locus_tag="PL000_16860"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNC90602.1"
                     /translation="MANFTNARAFMKAEKIPDIIVGRLPVYLRALQRMADNGLKTTSS
                     QELGEHVGISAAQIRKDISQFGDFGKQGTGYSIAYLLDKLREILKVDRIWEVALVGAG
                     DMGHALARYQGFTNRGFHIVMVFDNDKEKIGQKINGFTIEDTEKMSERLKSAGIKIAM
                     LTVPAPAAQSVTDKLVQAGVKAILNYAPINLNVPNGVKVQYIDPSTHLQRMTYYL"
     gene            7728..8573
                     /locus_tag="PL000_16865"
     CDS             7728..8573
                     /locus_tag="PL000_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC90603.1"
                     /translation="MKIFGKRLFRPRSIWIRKIVQWFFFLLIALISVNHTLVENGGGI
                     PFLSTASLHALCPFGGVVTIYQYATVGTFVQKIHESAFILMIIGFVLAFLFGPVFCGW
                     VCPLGTIQEWVAKLGHKFFKRKYNHFIPAKLDSVLRYARYIVLAWVVYVTATSGTLIF
                     AEYDPYFALFNFWSTEVAVGGLIILGATLILSLFIERPWCKYACPYGAVLGITNLFRV
                     FKIKRAESTCKADGACSIMCPMNIAVDTVQTVRDHQCISCLECTSEAICPVAKTVVFT
                     TGAVK"
     gene            8570..9346
                     /locus_tag="PL000_16870"
     CDS             8570..9346
                     /locus_tag="PL000_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90604.1"
                     /translation="MNLTSKTLAAIILVILFGGIVFSTAMGWWATESSKVAATYTEGE
                     FAGQANPADIRGSYTFGDVEKNFGIPAAIMAQAFNIQTENPAAFAAKELETIYGESEF
                     EVGTASVRLFVAFYNGLPFDLSTDMYLPQSAADLLKSRNLTAEQSAYLESHSIPNLAA
                     ESDSPEVAPAQTAESAPEAEATQAVVTTEASTERLVKGKTSFQEVLDWGVSQEIIEQI
                     MGMPMPNPLTKIKDYCLEKGLDFETIKTALQAEVDKAGQQ"
     gene            9430..10092
                     /locus_tag="PL000_16875"
     CDS             9430..10092
                     /locus_tag="PL000_16875"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90605.1"
                     /translation="MSKIWNASEYDLSRRRLIPCYDLFYSTAAELAARSVKDAAPVIL
                     DLGAGTGLLSEFVLNKIGPASLYLLDESAEMLAKAQQRLAEYNPLVFIQQMTGPLPSA
                     KFHAVISSLAIHHLTDEDKRDLFKRIHESLLPGGVFINAEQILGPTEWQDQMFEDMHL
                     NGARALGSDENEIRAAQERMKADRCATLEEQVAWLRDVGFKNSGTFFQHFRFAVYAGW
                     KG"
     gene            10179..10382
                     /locus_tag="PL000_16880"
     CDS             10179..10382
                     /locus_tag="PL000_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90606.1"
                     /translation="MRKRNWRLVGFGIFLLILAIGFYFFMATIAPTSLDPVALMKIVG
                     STSGTVGGLSIAIILIGLIGKKA"
     gene            complement(10434..11441)
                     /locus_tag="PL000_16885"
     CDS             complement(10434..11441)
                     /locus_tag="PL000_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNC90607.1"
                     /translation="MALFSKDLGVDLGTMFTRIADGAQVLLEEPTIVAIEVDDQKMVA
                     VGMEARDMFGRVPESIEVARPLRNGVIADYEISETLLQYLLQRVSGSMRIFRPRVMIS
                     VPYGVTSVERRAVYEAVMEAGSREAFLIQQPLAAAIGIDLPINSPSGNMLISLGGGCT
                     ETAVMAMYGIVAADTLRAGGMDLDEAIVNYVRRKYGVVIGQATAEQLKIRIGAAVPQD
                     TENSMEVQGQDQVTGLPRPVTLTTGEIVEALQEPLKQIVESGRKVLEKTPPELVSDII
                     DRGVALCGGGALLRGVDKLLTKSLGIPAYLVDNPLTCVVQGAVKSFGMLNVIRRNLPQ
                     V"
     gene            complement(11447..11887)
                     /locus_tag="PL000_16890"
     CDS             complement(11447..11887)
                     /locus_tag="PL000_16890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNC90608.1"
                     /translation="MTIRCEIVSQDRTVFTGDVDIVVLPGAGGEMGVLPKHAPVLTTL
                     KYGIVRVRKAGKEELFTVAGGVAEVQPDIVTILADAAENVEEIDEARADAARKRAEEA
                     LKKGLPADTDAYLAMEAALRKSNLRLDAVRRYRKNSRSPYSSEQ"
     gene            complement(11956..13356)
                     /gene="atpD"
                     /locus_tag="PL000_16895"
     CDS             complement(11956..13356)
                     /gene="atpD"
                     /locus_tag="PL000_16895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC90609.1"
                     /translation="MANANGRVVQILGGVVDVEFPNGDVPGLFEAIEVNRENKEPLIL
                     EVQKHLGNSWVRTVAMDSTDGLQRGLPARSTGAPITVPVGPATLGRIFNVLGNAIDKK
                     GDVQAASHYPIHRAAPAFEEQSTRVEVFETGVKVIDLIAPFTKGGKTGIFGGAGTGKT
                     VIIMELIRSVASVHEGNSVFAGVGERTREGTGLYREMIEYGVMKDTVMVYGQMNEPSG
                     VRLRVALTALSMAEYFRDQGKDVLLFIDNIFRFSMSGSEVSALLGRMPSAVGYQPTLA
                     TEMGELQERITSTRTGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSIVEKGI
                     YPAVDPLASTSRILDPNIVGEEHYRTAREVQRVLQRYKDLQDIIAILGIDELSEDDKL
                     VVSRARKIERFFSQPFFVAERFTGISGRYVTIQDTVRGFREILDGKCDDLPEQAFMMA
                     GTIEDVRERAEKLSKA"
BASE COUNT         3380 a         3638 c         3254 g         3132 t
ORIGIN      
        1 tatttccctt tcgcgccaag ataatcgctg tagccgcctt gatacccgcc cagctgacca
       61 tcttcgatga ccagtaacct ttccactgtg cggtcgagaa aataacggtc atgagaaatg
      121 acaagcaccg ttccttcgaa atcttcaagg gcttgttcca aaacttcagc agaggcaata
      181 tcaagattat tggttggttc atcgaggagc aggaaattcg ccccagagag caccaccaaa
      241 gccaattgca ggcggctgcg ttccccgcct gagagttcgc cgatcttttg cgagacctgt
      301 ttgtaggtga ataaatatcg caaaagaaat gccgtcgccc gtgattcgct catctcaccc
      361 gcataacgca cagcttccac aacggtttgg ttgaaatcga gggtttcatg ttcctgagcg
      421 taatgcccaa tggaaatgct gggaccgatc ttgatctcgc ctacatcggg cgtttctttt
      481 ccaaggatca tgcgcaatag aacagacttc cctgcaccgt tcgggccgat caaacccaca
      541 cgttcgccat gccagatggt ctcgttgagg tttgcaaaca cctgcttcga gccaaaggat
      601 tttgaaactc ctgcaagttc aagcaccttg ttcgaaccgc gccagccatt caaccgcaac
      661 tccattcgtt tgcgatcaag tacgggcttc tcgatcttgt ccattttatc gagccgcttt
      721 tgcatggtct tggccttggc ggcaaatttt tcgttgtcgt aaatctttcc ccaaatggca
      781 aagcgcttga tcgcaatttg catgcgggtg atctcatgct gctgcgcgcg gaaaagttcc
      841 tcctgccgtg ccatacgcgt ttccttgtct gcgatgaagg aggaataatc gccctcaaag
      901 acggtcaact tgccatcttc caattcagcg atatgtgtca cggcggcatc gagcagataa
      961 cggtcatgtg aaatcagcac taccgtccca tcgtattcgc ggatgagttt ttcaaggaac
     1021 atcttgccgg ggaggtcgag gtgattatca ggctcgtcca gcagcaaaat atcggggcgc
     1081 accaaaagca ggcgtgccaa cccgaccaat tttttctgtc cgccgctcaa aatagacagg
     1141 gacttgttta attcactctc agccagaccc agcccgagca acatgctgcg tacgcgcgca
     1201 ggatacgaat caccgccaag ggcatgatat tcttcaataa gtttgtgctg caactcaagc
     1261 gcctgctgaa gcctgcgctc gttgccatag acctcagcat cgcccaaact ggcttccacg
     1321 cgttccagat cagcctgcac ttctgccaca cgcggattcc ctgcaagcgc ggcttcaaac
     1381 gccgtcagcg taagatcaag ctcgggatgt tgagacaggt atcccgttgt aatgaggcgg
     1441 gcgcgggtga tcgatccgcc caattcggga gaatgttccc cttcgataag tttgaagaga
     1501 gatgacttcc ccgcgccatt cgcaccgatg agaccgatct tctggccgtt ctggatctcc
     1561 caattgagat tttcaaagat gcgctttgcg ccaaggacaa gtgtaatgtt tgaaagttga
     1621 agttggatca tgttgacctt taaataaaaa atgccgcagt cggcctgcgg catgtgaaag
     1681 ttgacaaaag gctttcagcg tacaggcgcg attccagaag gctgcgcgga aatgaagcca
     1741 cgtacgccaa tgacaagggg aaatttgcct gcactgaatt tgtaaatcat atgcgcgatt
     1801 ataactgaaa actgtggcaa aatatagcca tgcctgacca actcaacacc ctcagccagt
     1861 cttcgcttca agattattac gattgtccgc gccgatttga attgcgtcat ttacagcacc
     1921 tggtttaccc agccgttgaa accgaacctg cgcttgaaaa tgaaaaacac cagcaggaag
     1981 gcgaatactt tcaccgcctt gtgcagcagc atcttatcgg catcccagcc gagcaggttg
     2041 gcaggctcgc aaacacaccc aacctgcagc gttggtggga aaattattta agtgcaaaag
     2101 acctggcgag cctcagaaat cattcaaagc tatacgctga gacaactcta tctgcgccgc
     2161 tcggaaacct tcgccttgtt gcaaaatatg accttatcgc ggtaagcagc gaacaggtat
     2221 tgatctacga ttggaaaacc taccgcaaac gcccaaagaa tgactggttg agcatccgct
     2281 ggcagacgcg ggtgtaccgt gccctgcttg tgcaggcggg cgcgcatttg aacggcggaa
     2341 aaccgtttgc gcccgaacag atcgaaatga tctactggtt cgcagatttc cccaacgaac
     2401 cagcccgatt cacctacaaa gccgaacaat tcaaacgcga ttgggatgca cttgtaaaaa
     2461 tttcggatga aataaaaacc gcatcagact tcccgaaaac tgatgatgcc tcgaaatgca
     2521 gttactgccc ctacagatcg tactgcaacc gcggcattcg ggcaggtgat gccgccgatg
     2581 cagaactgga aaccgaagcc gaggaattgt tcgatatcaa tttcgagcag atcggtgaaa
     2641 tcgcattttg agctcaggct taaagaaaaa gcgccaggca aagtcgctta acagactggc
     2701 gattttgaaa aaaggcaaat tttttattgg cttttcgcgg ccccgcattg aagggtttct
     2761 ggctttgcac agccataggc attcgccatt gtgccgctat ggtaaaattc ctctcgcccg
     2821 cccccgtagc tcagtggaca gagtgtcggc ctccggagcc gaagagcgca gttcgagtct
     2881 gcgcgggggc gcctgttttt gcatgcagag ggatttctca agtgagggcg tgcaatttga
     2941 tcttctggat gcattcaagt ggcccttaga aacattctct ttattatctt tgtaatgctt
     3001 gtttgcacag gctgcggtgt gaaactcacc ggagaaggtg catccacagc agcgccagat
     3061 tttgttacct ccaccctgcc tccctcggca cctcccctgg caacacaaac cccaatcgca
     3121 caacaatcca tcgcccctac agcaatggcc acccacctgc ccgctgaagg aataacgaat
     3181 acgcaggtga atatccgcgc agctacatcc accgcaagca catcgtttgg ggtgatcggc
     3241 gcatttgaaa cggtgcaggt ccttggcaaa gatgccagcg gaagttggta tcaaatcatg
     3301 tacgcagggt cgcccaatgg aaacggctgg gtccgcgccg agtatgtgca ggtcaatgat
     3361 gcggggaaga tcctgcctgt tgaaggcctc acaggcaacg gcgggcttgt cctcgaagcc
     3421 gtcaacctcc gcagcggacc agggtcagat tttgagtcgt tgggtgttct cagttcaaaa
     3481 gatgcggtac tcgtcactgg caaagacccg agcggtgcat gggtgcaggt cgaagtgaca
     3541 ggcgcggaaa atttgaaggg ctgggtcacg gtcaaatttt tgcaggtgga aaaccttgag
     3601 gcaatccctg tgatcgggga aattgccagc ccaacatccc tccccgccga agaagtttcg
     3661 acggccattc cgccagcctc acaggatgga gactcgcttc aatctccgct gacagttgtc
     3721 cgcttttctt caacagaatc acgggccatg cagattcagg gcaatgtctc cgcccccgct
     3781 ggcgacacgg aagattggat acagttttca acaggcggca atggtgtcgt aattgaggcg
     3841 ctttgctcca gtgatacact caccatagaa ttgtggtttg acggacaacg cacggatggt
     3901 ttttctcaga tttgcgggga aaaacaattc gtaactgttg agccgaacgg aaagtacgtt
     3961 ttacgaattc tcgaaaccag cacaaatcag tttcgaaaca cagatttcat tttgaccata
     4021 gaggacattc atcaaccatg aaaagcttac caagcatcga caccatgggc tcacgcccgg
     4081 atattattcg caataaacgc atctttggaa tgatctacgg aatcatggca gggttggcct
     4141 ttgctggctc ttcctggggc tgggatgggt acacgctctc ccaattccac gggtattttc
     4201 cgtggaatgt gctggtcaca ggggcgcttc tgtgcgccat tgtcggcggg ttcattggct
     4261 ggttgacggc ccgcatggaa agcagcctgg caggcgtgct attctggctg ggaggatcgt
     4321 ttttcttcgc atggctcacc gttgccctgc cgttgcaaat ctcccccatc attgccacga
     4381 aaatggatcc gcaactggca ggcctgctgg attacagcag taacatcgaa ttcacctccc
     4441 gttttggagt ggcactcgcg tggatcctgc catttaccgt gatcgtgggc atcgctcaaa
     4501 tccctgtcac agaacctgcc gtattttcaa catcgttttt tggaaagatc ggtccgctcc
     4561 tgtttgggat gctggttatc agcatcggcg gcgtcatcac agacagtttg atcaatgccc
     4621 agtttcgaag cgccatcatt gcactcgaca aaacggccca attcgttttg gataaccgcg
     4681 gcaatgaaaa tgtggatccg ttggttgcgc gtgaaataca tgcccgcgcc tttggaaccg
     4741 tggaagaaag catgaccgac acaagatatt tattcgttgg aagtttcgac gaaaccctgg
     4801 gagatatcaa cattctggta aagtttgaaa aagcatgggt gacctgcgag gtcctctaca
     4861 accagccgat cacatgcaag ccagtccaat cagaataacg aggaatacaa agcatggaaa
     4921 aatcgccaaa gactttcaac ttgcgctttg ttctgctttt ggggacggtc attgtcttat
     4981 tgttgatcca attttctgcg cttctgccga atgtgaccac ccctgtggag cgcatcgaat
     5041 attccacgcg cgatgtgttg atgcgcctgc ggggcgcaca ggacccctcc caaaacatcg
     5101 tgatcgtgga aattgacgat ttctccttta attggacggg gtatcaatgg ccctggcccc
     5161 gatcgtatct tgctgggatc gtagaccaaa tcaacaaggg tggcggcaaa gtggtgggcg
     5221 tggatatttt cctctccgaa cccgatgaag atactgcaaa tgaccaggcc tttgcgacgg
     5281 cactggcaaa tgcgcccgct gctgtgggcg tggtgcagat attcgaaagc cctgtcgata
     5341 ctttttcgat ttccaccttg ttgcaagccc aacctgttta tcagtccgct ctggatgcaa
     5401 acggcatcac cgccttcacc cgcgatgaag atgccatcgt ccgcagcgtg caggcatatg
     5461 atttctacaa ggacaaagtt tattatcact gggcctttga ggtcgcaagg ctgtatcttg
     5521 atgtcgaacc accgacccag cctacggata ccagtatcca gttcaacggt cagagtgtgc
     5581 cgctgcgtgc ggggcatgta ctggtcaatt ttgcgggtcc atccagaacc tacaccacct
     5641 atcgcgcggc cgatgtccac gatgggatca cgcttgaaga aaatccagac gcgtttcgcg
     5701 gcaagatcgt cttgatcggg gcaacgaccg tcaccttgca ggatatgtac cccaccccct
     5761 tttccactca gatcccaacc gcaggcgtgg aaattgttgc cagcaccata gatacgctga
     5821 tcacaggtca atacctgacc gaagcgccgc cctggctggc gttggcactg accatcctag
     5881 ctgctgtgat cgcctcgctg atctcccgct ccaatcgccc aacgttaaca attgtgctgc
     5941 ttgtgttggt aatggcaggt tatgcggtag gcggcgtggt gttattttta cgtcagagat
     6001 acatcctgcc attcgtcgta ccgcaggtca tgttgttttt ggggattgtc ctgcctactt
     6061 tggaacaggc ggtttcgcag gagttggaaa agcgccgtgt cagaaatctt tttagccgtt
     6121 tcatctcccc cgaaatggtt gaccagatga tgtccactca ggatctgaac tcactgaaca
     6181 aacgctccaa catcagcatc atcttttcag atattcgcgg atttaccact ctttcagaaa
     6241 agctcgcgcc tgaagatgtg gtggccttgc tcaaccccta tttggaagcc atgtccaagg
     6301 tcatttacaa acatggcggc accgtagaca aatacgaagg cgacgccatc attgctttct
     6361 tcggcgagcc tgtcccgttc aaagaccatg ccatccgcgc cctgcgcgcg tcactggata
     6421 tgcgaaaggc gctggatgaa ttaaaagcac aatgggaaaa agaaggccgc cccacccaga
     6481 tcgaaatggg cattggcatc aactccggtg acgttttcgt aggcctgctt ggctcggcac
     6541 agcgtatcaa ctacaccgtg atcggcgata atgccaacct cgcctcccgc ctgcaagacc
     6601 tgacaaaaac ctatgcgtgg ccgattctca tcagcgaaag cacctaccaa caggttaaag
     6661 atgagtttga tgcggagttc gcggatgcgg tcacggtcaa agggaagaca aaacctgtaa
     6721 atgtgtacaa agtacttggt tacaaaggcg caccacccgc tgagcagcta caatcctggc
     6781 aaaagtagca aaaacgaaaa aacggcaccc taaaaaatgc cgttttttcg tttgtacgat
     6841 acttgtgtgg aattgtacaa aacctctttt tatggtagag ttatggcgaa tttcacaaac
     6901 gcgagggcat tcatgaaagc tgaaaaaata cctgatatta tcgtcggccg actccccgtt
     6961 tatctacgcg ccttacagcg catggcagat aatggcctga agacaacttc atcgcaggag
     7021 ctcggggaac atgtaggcat ttccgccgcc caaatccgta aggacatctc gcaattcggc
     7081 gatttcggca aacaaggcac ggggtattcc atcgcctacc tcttggataa attacgtgaa
     7141 atccttaaag tagatcgtat ttgggaggtg gcccttgtag gtgcaggtga tatgggtcac
     7201 gcgctggccc gttatcaggg ctttaccaac cgcggctttc acatcgtcat ggtcttcgac
     7261 aatgacaagg aaaagatcgg tcaaaagatc aatggcttta cgatcgaaga cactgaaaaa
     7321 atgagtgaac gcctgaaatc ggctggaatc aaaattgcaa tgctcaccgt cccagcacca
     7381 gctgcacaga gcgtcaccga caagttggtt caggctggcg tcaaagcgat cctcaactac
     7441 gcccccatca acttgaatgt tcccaatggt gtcaaagtac aatacattga tccatccaca
     7501 catttacaga gaatgaccta ttatttataa aaagtggaaa acaggggcaa atcaaaatca
     7561 caaaaacctg ccgaacatat gcgttcggca ggtttttctt taccttccac acaatctcta
     7621 attacctgct tcccaaaaaa cgctttaccg aatcttcata ttctctttga acggcctaaa
     7681 cacaaattct ttacactatg tccaataaaa agaacaagga gaatatgatg aaaatctttg
     7741 gtaaacgcct cttccgtccc cgcagtatct ggatcagaaa aatcgtgcaa tggtttttct
     7801 ttctacttat tgcgcttatc tcggttaacc acacattggt ggaaaacgga ggcggcattc
     7861 ccttcctgtc cactgcatca ctgcatgcac tctgtccttt cggcggcgtg gtcaccattt
     7921 accaatatgc cacagtgggt acctttgtac agaaaatcca tgaatctgcg ttcattctca
     7981 tgatcattgg cttcgtgctg gcctttcttt tcggccccgt attttgcggt tgggtttgcc
     8041 cgcttggaac cattcaggaa tgggttgcca aactgggaca taaattcttc aagcgaaaat
     8101 acaaccactt catcccagcc aaactcgatt ccgtcctgcg atatgcacgc tacatcgtcc
     8161 tcgcctgggt ggtgtacgtc accgccacca gcggcacact gatctttgcc gaatacgacc
     8221 catattttgc cctcttcaat ttttggagca ctgaagtagc cgtcggcggc ttgatcatcc
     8281 ttggcgccac actcatactt tcgcttttca tcgaacgccc gtggtgcaaa tacgcctgtc
     8341 catacggagc agttctggga attaccaacc tcttccgtgt tttcaagatc aaacgcgccg
     8401 aatccacctg taaagccgat ggcgcctgct ccatcatgtg cccaatgaat atcgccgtgg
     8461 atacagtcca aaccgtacgc gatcaccagt gcatctcatg cctcgaatgc acatccgaag
     8521 ccatttgccc tgtcgcaaaa actgttgtct tcaccacagg agccgtaaaa tgaacctcac
     8581 atccaaaaca ctcgccgcga tcatcctcgt cattttattt ggcggcatcg tcttcagcac
     8641 agccatgggc tggtgggcca ctgaaagcag caaggttgca gccacgtaca ccgagggaga
     8701 atttgcaggt caggccaacc ccgccgacat ccgcggctcg tacaccttcg gcgatgtgga
     8761 aaagaacttc ggtatccccg ctgccatcat ggcgcaggca ttcaacatcc aaaccgaaaa
     8821 cccagccgcc ttcgccgcca aggaactcga aaccatctat ggcgagagtg aattcgaagt
     8881 aggcactgca tctgtcagat tattcgtggc tttttacaat ggcctgccgt tcgacctcag
     8941 caccgacatg tatcttccac aaagcgcggc ggatctgcta aaaagcagaa atctcaccgc
     9001 agaacaatct gcttatttgg aatctcacag catccccaac ctcgccgccg aatcagactc
     9061 acccgaggtt gctcctgccc aaacagcaga gtcagcacct gaagcagaag caactcaagc
     9121 cgtagtcacg accgaagcat ccactgaaag actcgtcaaa ggcaagacat ccttccagga
     9181 ggttttggat tggggcgtga gtcaggagat catcgagcag atcatgggta tgccaatgcc
     9241 caatccgttg accaagatca aagactactg cctcgaaaaa gggttggatt ttgaaaccat
     9301 caaaacagcc ctgcaagccg aagtagataa agcagggcag caatagccat cagaaaacag
     9361 gcacaggcgg ttcaaaacga accgcctgtt tattttcaaa aaagtgaatc gaagatacaa
     9421 tcggccgcta tgagcaaaat atggaacgcc tctgaatacg acctttcacg ccgccgcctt
     9481 atcccctgct atgatctgtt ctactccaca gccgcagaac ttgctgcccg ctcggtcaaa
     9541 gatgcagcgc ctgtcattct tgatcttggc gcagggacag gtctgcttag cgaattcgta
     9601 ctcaataaga tcggccctgc ctctctatac ttactggacg aatccgctga gatgctggcg
     9661 aaagctcaac aaaggctcgc ggaatataac ccgttggtgt tcattcagca aatgaccggg
     9721 cccctgccgt ctgccaaatt ccacgccgtc atttcctcac tggccattca tcaccttaca
     9781 gacgaagaca agcgcgatct tttcaaacgc atccacgaat ctctgctgcc tggcggggta
     9841 ttcatcaacg ccgaacaaat cctgggaccc accgaatggc aggaccagat gtttgaagat
     9901 atgcacctca acggcgcgcg cgctctgggc agtgacgaga atgaaatccg cgctgcacag
     9961 gagcgtatga aagctgacag gtgcgccacg cttgaagagc aggttgcatg gttgagagac
    10021 gttggtttca aaaactcagg gacattcttt cagcatttcc gttttgcagt ttacgcaggc
    10081 tggaaaggat aatgattttt tcattcccta aactactttg cattgggtaa aatcaaaatc
    10141 cagagacaaa atccattatt aaaaggagtc ttacaaatat gagaaaaaga aactggagat
    10201 tggtcggctt tggaattttt ctacttattc ttgccatcgg gttttatttt ttcatggcaa
    10261 ccatcgcccc cacctctctt gatccggtcg ccttgatgaa aattgtaggc tctacctcgg
    10321 gaacagttgg aggcctcagc atcgcaatca tcctcattgg gctgatcggc aagaaagcat
    10381 aaaaagccag ccagtacgca caaaaaaggc tcccatcagg gagcctttga tttttacacc
    10441 tgcggcaggt ttctacgaat tacgttcaac attccaaaac tcttgaccgc gccctgcact
    10501 acacatgtca gcgggttatc gaccaaataa gctggaatgc ccaaagactt ggtcaacagc
    10561 ttatccacac cgcgcaagag cgcgccaccg ccgcacaaag ccacgccgcg atcaataata
    10621 tcggaaacca gttcaggcgg ggttttttcg agcaccttac gcccagactc aacgatttgc
    10681 ttcaacggtt cctgcaaggc ttccacaatt tcgcctgtgg tcaaggtgac gggccttggc
    10741 agacctgtga cttgatcctg cccctgcact tccatgctat tttcagtatc ctgcggtacg
    10801 gccgcaccga tacggatctt caattgctcg gccgttgcct gaccaatcac cacgccatat
    10861 ttgcggcgca cataattgac aatggcttcg tccaggtcca tgcccccagc acggagcgta
    10921 tctgcggcaa caattccata cattgccatt acagcagttt cagtacaccc accaccaagg
    10981 ctgataagca tattgcctga gggggaattg attggcaagt caatgccgat cgccgcagcc
    11041 aaaggctgct gaatcaggaa cgcctctcgg ctgcctgctt ccatcacggc ttcatacact
    11101 gcacgacgtt caacgctggt cacaccatac ggcacagaga tcattacacg cgggcggaag
    11161 atacgcattg acccgctcac acgctgcaaa agatactgaa gtaatgtttc gctaatttca
    11221 taatcagcga tcacgccatt ccggagcggg cgggcaactt cgatactttc cgggaccctt
    11281 ccaaacatgt cacgggcttc catgccaact gcgaccattt tctgatcatc cacctcaatg
    11341 gcgacgatgg tcggctcctc caacagcact tgcgcgccgt cggcgatacg ggtgaacatg
    11401 gtacccaggt caacaccaag gtctttggaa aagagtgcca ctatgtttat tgttccgatg
    11461 aatagggcga acgtgagttt ttgcggtaac gacgcactgc atcaaggcgt aaattggatt
    11521 ttcgcaatgc agcctccatg gcgaggtagg cgtcagtatc ggcaggcaag cccttcttaa
    11581 gagcttcttc tgcacgcttg cgtgcggcgt cggcccgggc ttcatcaatt tcttcgacat
    11641 tttcagcagc atcagccaga atggtgacaa tatctggctg cacttcagcc acgccgccag
    11701 ctacagtaaa gagttcttct ttacccgcct tgcggaccct gacaatgcca tacttcaaag
    11761 tggtgaggac aggcgcatgc ttgggaagca cacccatttc gccccctgca ccaggcagga
    11821 caacgatatc cacatcaccc gtgaacacgg tgcgatcttg agaaacgatt tcacaacgaa
    11881 tggtcatata aaaacctttt ggagattagg aaattggaga tcagccctgg ctgatctccg
    11941 atcgctaatt accaattacg ctttagacag cttctcagcg cgttcgcgca catcttcgat
    12001 ggtaccagcc atcatgaatg cctgttcagg caggtcgtcg cacttgccat caaggatctc
    12061 gcggaagccg cggacagtat cttgaatggt tacataacgg ccagagatgc cggtaaagcg
    12121 ttcagccacg aagaaaggct gcgagaagaa acgttcaatc ttacgagcgc gggacacaac
    12181 cagtttgtcg tcttcggaga gttcatcaat accgagaatg gcgataatgt cctgcaagtc
    12241 tttgtagcgc tggagaacac gctgcacttc acgagctgtt cggtagtgtt cctcacccac
    12301 gatgttagga tcaagaatac gggaggttga agcgagcgga tccacggcgg ggtaaatacc
    12361 tttttccacg atggagcgtt cgagggcgat ggtcgcatcc aagtgggtaa aggtcgccac
    12421 aggagcggga tcggagtagt catccgcggg cacgtacaca gcctgcatgg aggtgatgga
    12481 gccggtgcgg gtggaggtaa tgcgttcctg cagttcaccc atttcagtag cgagagtcgg
    12541 ttgataaccc accgcagaag gcatacgtcc aagaagcgcg gacacttcgg aaccagacat
    12601 ggagaagcgg aaaatgttat caatgaagag caacacatcc ttgccctgat cgcggaaata
    12661 ttccgccatc gagagcgcgg tcaacgccac gcggaggcgt acaccggaag gctcattcat
    12721 ctgtccgtag accatcacgg tgtctttcat aacgccgtat tcgatcattt cacggtaaag
    12781 accagttcct tcacgggtgc gctcgcccac gccagcgaac acagagttac cttcatgaac
    12841 ggaggccacc gagcggatga gctccatgat gataacggtc ttgcctgttc ctgcaccacc
    12901 gaaaatgccc gtcttaccac ccttggtgaa gggggcgatc aggtcaatga ctttgacgcc
    12961 agtttcgaag acttccacac gtgtggattg ttcttcaaac gcaggcgccg cacgatgaat
    13021 ggggtaatga gatgccgctt gaacatcacc cttcttgtcg atcgcattgc cgagaacgtt
    13081 aaagatacga ccaagggtcg cgggtccaac cgggaccgta atcggggcac ctgttgatct
    13141 ggcagggagt ccacgctgga gaccatctgt cgaatccatg gccactgtac gaacccagct
    13201 attgccaaga tgtttttgaa cttcaagaat aaggggttcc ttgttttcac gattcacttc
    13261 aatcgcttca aagagcccgg ggacgtcacc attggggaat tcgacatcca ccacaccacc
    13321 taaaatttgt acaacacggc cgtttgcatt tgccatgatt tcctccagtt atttcgcggc
    13381 cagcgcttct gcgccgccga caat
//
LOCUS       DAOQLC010000117        13230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1753_length_13230_cov_22.314839, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000117 DAOQLC010000000
VERSION     DAOQLC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13230
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1753_length_13230_cov_22.314839"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PL000_16900"
     CDS             <1..1028
                     /locus_tag="PL000_16900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90610.1"
                     /translation="TLILSDLGSVTATLIALLLYHAGSLALWHIYLLSLFAGIFTAFQ
                     YPAYIAATTALVSKDDYARAQGMVGLAQAASTIFAPMIAAALLPSIGLSGIMTIDLLT
                     FVFAFSILLWIKFPLRSDLTPAPVTQTGFAREVSFGFRSIFANPTLRALTILFVVANF
                     FLAIGATLLAPTILSHTANSEGALAAILSVGAAGGLIGGGLLSVWGGPKRKVNGILLG
                     GVAACLIGISLFGLARSVLIWGIASFFFSFFEPFVEGSNTALWQSTVEINAQGRVFSA
                     RQLLTQIPYLIGMAGSGWFAELGIARFTGTQNGIHLSTTLVLAGLAGSAVFIIGYFVP
                     PIREIQE"
     gene            1063..1896
                     /locus_tag="PL000_16905"
     CDS             1063..1896
                     /locus_tag="PL000_16905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90611.1"
                     /translation="MITWIIGIAVVLVLLFLLDREIYRYEGIHIGPRLQAWLYDRWAE
                     KYDHDKKDGQLQDVEMLAKPLLTKLTEVPEPFILDVATGTGRLPFVVASQPEFTGHII
                     AVDISHGMLKQAAKKIAPHQSRVELMKYDSFPLPFPDETFDVVSCVEALELIGEKQAA
                     IQELARVLRPGGTLLTSRGTEESGRGIVVISAEMLTSLLKTSGFEDIEITPWWRVFNR
                     VFARKPGALAPAGHKSVKDVLLCQQCKRAGNFSKTEASFTCGHCQKQVHVDEYGIAIF
                     R"
     assembly_gap    2055..2154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2255..2659
                     /locus_tag="PL000_16910"
     CDS             2255..2659
                     /locus_tag="PL000_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90612.1"
                     /translation="MANKTFLYIGAGILLLIGLCLLGYGLLNVIGSTSAQGSSSWLTF
                     GLGFSCVGVFFLAGGIGLIVFASRQKGPAGSGDQITYKIDLPAQTKVEQMKCNSCGGA
                     LKADNVKMIAGAPTVECPYCGTVYQLTEEPKW"
     gene            2683..4350
                     /locus_tag="PL000_16915"
     CDS             2683..4350
                     /locus_tag="PL000_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90613.1"
                     /translation="MKRKLLLLSFILILVLAAFSNASAQDAPYYFEVNKEVVHVYWNP
                     DGTMSVDYTWYFNNLPGSHPIDFVDVGMPNYNFNTSTIVAESDGMDLMVTTQDYQGSG
                     SGFAVVMGSRTIQPGDKGILHVFVPNISSVLYNDANDANYASAVFAPTWFGQEFVSGN
                     TDFTMTFHLPPNMNPEEPRWHEAPSDFPETPQAAYDSENRVTYTWYAPDATAYNYYSF
                     GASFPRIYVPAEAIYTPPPEPLFNIDEDTIFSLLCGGFFFFIFFISPAIAAITGRRRK
                     MQYLPPRVAIEGHGIKRGLTAVEAAILMEQPLDKVMTMILFSVVKKGAAQVETRDPLK
                     LSVAAEQPATLHPYEKDFIKAFAVDEKRSRVLQDMTVALIKSVSEKMRGFSRKETVEY
                     YKSITEKAWAQVEAAGTPEVKSQKMDESLEWTMLDKDYDDRARRVYNGPVYVPTWWGR
                     YDPSYRRISTPTTVSGPTISSTPRPSSSGQALPGADFAASMVTGVQTFSQKVIGNVQS
                     FTERVTGATNPLPKSSSSGGRSGGGGRSCACACACAGCACACAGGGR"
     gene            4359..4892
                     /locus_tag="PL000_16920"
     CDS             4359..4892
                     /locus_tag="PL000_16920"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC90614.1"
                     /translation="MLGAIFKKAPHTPPHLFVPNALYMLTASTYRSEPFMQTTERKLN
                     WIKTFLTSAEMYKWEVVAWVVLDNHYHAVLKAPDSADNLPKFVASYHRFTARNWNEEE
                     QASGRKVWWNYWDTCIRSEYDYYNRLRYTFWNPVMHGLVKDPNDYPFSSYKVFLSKWK
                     VKFNFTNMEEVKNVPEF"
     gene            4876..6273
                     /locus_tag="PL000_16925"
     CDS             4876..6273
                     /locus_tag="PL000_16925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNC90615.1"
                     /translation="MSLNFSSLKNILSLPFQQAPKADAAETETRLYHYVHENEMEKSR
                     IHLRIDPDGTGTLIVNASSVMHLNPTAAFMAWLILEGKTEDERVQALRARYSVSKKQA
                     REDLAEFNYQLEEMIRPDGACPVHELDLETKMPFSARPSAPYRMDLALTYRCNNDCAH
                     CYNARERNFPELTTQQWFKIIDQLWELGVPHIVFTGGEATLRNDLPELIHHAESNGQI
                     TGLNTNARRLMDMDYVKKLVNAGLDHVQITVESSVPEIHDEMMRAKGAFRQTIAGLNN
                     VLASELYVMTNTTMLRTNVHTIPATLDFLSDLGVPTIGLNALIYSGHGLTVGTGLLES
                     ELQPILNIATQKTAEHGQKLIWYTPTQYCQFDPTAHNLGVKGCTAALYSMCIESNGNV
                     LPCQSYYHPLGNMLTDSWDSIWNHKLSTQLRERQNLPFKCETCSLVAECGGGCPLQFE
                     NHREKFGVNLPVLTL"
     gene            6329..6670
                     /locus_tag="PL000_16930"
     CDS             6329..6670
                     /locus_tag="PL000_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90616.1"
                     /translation="MNDHAWIKEFPAAITVCDDEGIILEMNDKAAKTFEKDGGYKLVG
                     SNVLDCHNDPSRGKLERLMSAREKNVYTIEKNGVKKMIFQSPWYKDGIYAGFVELSLE
                     IPFEMPHFIRS"
     gene            6685..7695
                     /locus_tag="PL000_16935"
     CDS             6685..7695
                     /locus_tag="PL000_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90617.1"
                     /translation="MEICYLIAFPDTDDGQNPAPERFKGLKDAPYFQPIDIDLVTLGE
                     ETLIIEGHAVQVFRQRYDGCVQMIECHFDLKDPFAASVLQERAKIQSALESKFVPTHY
                     RQNGLFEEYTILLVKEAKPTPDKWIEKNALALTKFIRSQREVFDKSEIEEILISRTHY
                     SAVELTLIDWEGAIIIAPNEDYQSDIALLKIGNYQLLRYRMLDQSIENTLDKINETFF
                     EKKRGLRPTRGVIRQIAEHKLEVMLDFERAEQNLLLIGDWYTAKLYEAIQSEFYLKDW
                     KENVKTKLDNLENIVQTIRDNFSLSLESVWGRIEMLGWMIMLIGYAYLFLADMGILKI
                     GK"
     gene            7717..8847
                     /locus_tag="PL000_16940"
     CDS             7717..8847
                     /locus_tag="PL000_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC90618.1"
                     /translation="MNFIQKIFTKTAPLPAGTHHMQAMHDEKPYRLHLRLHKDGSGLL
                     ILNAATVIQLNSTAAEYAYHFIKGTSPDEAAKQVAARYRVDKKTALADFNNFADQIQT
                     LISTPDLDPASFLNFERAQPHSTESTLRLDCAITYRLPESTHATYAPVKRVDRELTTT
                     EWQSILDKAWQAGIPHIIFTGGEATLREDLPQLIGHAEKNGQVCGLLTDGLKLADKNY
                     FETLLQTGLDHVMLILQPDEPKSWKAIETIVPQDIFLTVHLTLTKENINEAQNTLQKL
                     AYLDVQNISLSAADASLLDEMLELQDTASSLRLTLRWDLPVPYSAANPVSIETVDEET
                     PSGAGKTWMYVEPDGDVLPAQGEPDKILGNFLRDDWRTISKQ"
     gene            8858..9199
                     /locus_tag="PL000_16945"
     CDS             8858..9199
                     /locus_tag="PL000_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90619.1"
                     /translation="MKIIGTENMGSMELQMELQKGGKFIMYEYVISLLVITFKRNSNI
                     YFVKADESAVVKGLPFTLLSLLVGWWGIPWGIIYTIQAIFTNLSGGKDVTTDVLSAIR
                     ARQSAQNVNPA"
     gene            9230..10246
                     /locus_tag="PL000_16950"
     CDS             9230..10246
                     /locus_tag="PL000_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90620.1"
                     /translation="MKKNFVAVLALLLFSLACQTLFPKNTTPPDNNVTAEPTQRGTPS
                     PIVCTDDSCINACLERINNTLETRRFDSIGGDYAGAEANFDLVKYQVENGAISDPEIL
                     YVPKEFKPFQDDLQAQQTVWKYASSLLTENELKWITEYVVFTDGESNTLAWVNNKDDL
                     DLSRWQLGVDIIDAADPVDLTATLIHEFGHLITLNSEQFTPTDYYYGWNQNNSACPQF
                     TSPEGCSTPDSYINLFYQNFWIDIFDEWLETVENPQVDSDEEFDALRRAFYSKHSEQF
                     VREYAATNIREDMAESFMHFVLTPQPNGNNPVEQKMLFFYQFEELTTLRRQIIQNVCS
                     YTQE"
     gene            10248..10811
                     /locus_tag="PL000_16955"
     CDS             10248..10811
                     /locus_tag="PL000_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HNC90621.1"
                     /translation="MTKLPIPECYWVEENRLLAGEYPGHHDPEIMRRRIDAFLEAGAD
                     TFFDLTQAHELVPYEATLQEEARIYGIDASYQRFPIRDYGIPTANTMTAILNAIDEAT
                     SAGHCVYVHCWGGVGRTGLVVGCHLVRHGFTNQQAIAQVDTLFKTRPNRIFHPRSPES
                     DEQVDFILNWWEDATELHRNNKRFCEG"
     gene            10808..11527
                     /locus_tag="PL000_16960"
     CDS             10808..11527
                     /locus_tag="PL000_16960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90622.1"
                     /translation="MNWHTRYTQQANWTRELRAYLFEQAGMDRAKRILEVGCGTGAIL
                     STLNTSATRHGLDLEPASLAECRIHAPAALLSCADAHSLPYPDQSFDIVYCHFLLLWV
                     KDPLQVVREMARVGRTVLALAEPDYSQRVDEPATLKSLGVWQTESLRRQGADPSFGGM
                     LAETFHQAGIKLIETGPIQSQVVMRSAEDWETEWAVLQTDLEGIAQREEIQKMKVLDE
                     IARKNGDRTLYVPTYFAWGKT"
     gene            11586..12221
                     /gene="lepB"
                     /locus_tag="PL000_16965"
     CDS             11586..12221
                     /gene="lepB"
                     /locus_tag="PL000_16965"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC90623.1"
                     /translation="METLQHEEQPQSPIEGQVEGQIEGQPTQEAPAQEKIKWGRFFVD
                     ILETLVLAVILFFGINAVSARVRVDGYSMRPTLDDGEFVLVSKFNYKLSNVQRGDIIV
                     FHFPMDPKQELIKRVIGLPGDRVHVKDGVVYINNQALDEPYIAAPPAYTSMWNVPEDR
                     LFVLGDNRNDSSDSHSWGYLPFENVVGKAVLIYWPPPMWDVIEHKDLLVAQ"
     gene            12231..12527
                     /locus_tag="PL000_16970"
     CDS             12231..12527
                     /locus_tag="PL000_16970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HNC90624.1"
                     /translation="MSDNEYTPRSFPCNECHSGILQPRHITYFTWLGNELITVPHFPA
                     WVCDVCGKREYDEKAISWLNMILDPNAGKPNAKSRRPLPRPKLRTNNPRPAHDS"
     gene            12549..13103
                     /locus_tag="PL000_16975"
     CDS             12549..13103
                     /locus_tag="PL000_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90625.1"
                     /translation="MTLDTSPRTYRFLPADIMTSGYRVVGKIMITNNGVMGMMNDATH
                     SAMEVNDARLARLHMPTKLVDHFELIRMMKRQIHAVCLARREDLGPLALVRGGYASVV
                     EHPVRIATQMFEVEGTMELPGRFDFTAIMTDGTRTFLPVLNATLTAILIPNLRVESAS
                     ILVNRNLIDIMALINQRVKPEIQP"
     gene            13157..13229
                     /locus_tag="PL000_16980"
     tRNA            13157..13229
                     /locus_tag="PL000_16980"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13191..13193,aa:Glu,seq:ttc)
BASE COUNT         3429 a         3701 c         3087 g         2913 t
ORIGIN      
        1 ccaccctgat cctcagtgat cttggcagtg taaccgccac actgattgcc ctgctcttgt
       61 accatgctgg cagcctcgcc ttgtggcata tttatttact cagcctgttt gcgggcatct
      121 ttaccgcctt tcaatatcca gcctacattg ccgctacaac tgcactagtt tcaaaagacg
      181 attacgcccg cgcacagggc atggtgggtc ttgcgcaggc ggcatccact attttcgcac
      241 ccatgatcgc cgccgctctt ctgcccagta ttggactgag cggaatcatg accatagacc
      301 tgctcacttt tgtttttgca ttcagcatcc tgctatggat caaattcccg cttcgctccg
      361 acctcacacc tgctcccgtt acccaaacag gattcgcccg tgaagtttcc ttcggcttcc
      421 gctctatttt cgccaacccc actctccgcg cattgaccat tcttttcgtt gtggcaaatt
      481 tctttcttgc catcggtgcc actttgctgg ctcccaccat cctcagccac acagccaaca
      541 gcgaaggcgc actggcagcc attctatccg tcggcgcagc aggtggattg attggcggcg
      601 ggttgctcag tgtttggggc ggccctaaac gcaaggtgaa tgggatcctg cttgggggtg
      661 tggcagcctg tttgatcgga atcagtttat tcggtctggc gcgaagcgtt ctcatctggg
      721 ggatcgccag tttcttcttt tcctttttcg aaccattcgt cgaaggcagc aatacggctt
      781 tgtggcaatc cactgtagag atcaacgcac agggacgggt cttctcagcg cggcagctgc
      841 ttacgcaaat tccctacctg atcggcatgg caggttcagg ctggtttgca gaacttggaa
      901 tcgcccgctt cacaggcacg caaaacggaa tccatttatc aacaaccctg gtactggcag
      961 ggctggcagg gtcggctgtt ttcatcattg gttatttcgt gccgcccatt cgtgaaatac
     1021 aggaataagg ttttcgtcat cggcaattct tcaggagtct ttatgattac atggatcatt
     1081 ggcattgcag ttgtgctggt tctcttattt ttgttggatc gggaaatcta tcgttacgaa
     1141 ggcattcaca tcggtccgcg cttgcaggcc tggttatatg accgctgggc cgagaaatat
     1201 gaccacgata aaaaagacgg tcaactacag gatgttgaaa tgctggcaaa gccgctgctc
     1261 acaaagctga ccgaggtccc cgaaccattc atccttgacg tggcaacagg gacggggcgc
     1321 ctgccattcg tcgtagccag ccagcccgaa ttcacaggcc acatcattgc ggtggatatt
     1381 tcacacggca tgttgaaaca ggctgcaaaa aagatcgccc cgcatcaaag ccgcgtggaa
     1441 ttgatgaaat acgattcttt tccgctccca ttccctgatg aaaccttcga tgtcgtttcc
     1501 tgcgtggagg cattggagtt gatcggcgaa aagcaggcgg cgatacagga actggcgcgt
     1561 gtgctgcgtc cgggcggcac cctgctcacc tcacgcggaa cagaggaatc agggcgcggc
     1621 attgtcgtca tcagcgcaga gatgctgacc tcgcttttga aaacttctgg attcgaagat
     1681 atagaaatca cgccctggtg gcgtgtgttc aaccgtgtct tcgcccgcaa acctggcgca
     1741 cttgcgccag cgggacacaa atcagtgaag gatgtacttc tttgccagca atgcaaacgg
     1801 gcggggaatt tctcaaagac agaagccagc ttcacgtgtg ggcactgcca aaagcaggtc
     1861 catgtggatg aatatggcat tgcgatattt agataaaggt gacggggcga aataaaagac
     1921 aatgtctgta gcgcgagact gcccgaaggg cgtatctcgc gctacagcat ttacacaaat
     1981 ccattacctg gacaaaagct aatcggataa aagcaggaga atttcaagac gaaagtcaca
     2041 tggcgaaatg agacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaatcg
     2161 gataaaagca ggagaatttc aagacgaaag tcacatggcg aaatgagacg gaaatgctaa
     2221 acttttcata catcaaccta ggagatgtga ctttatggcg aacaagacct ttttgtacat
     2281 cggagcgggg atattgctgc tgatcgggct gtgccttttg gggtatggtc tattgaacgt
     2341 gatcggctca acctcggcac aagggagtag ttcctggctg acatttggcc tgggattttc
     2401 atgtgtgggg gtgttttttt tggcgggggg aatcgggctt attgtttttg caagcaggca
     2461 aaagggtcca gcaggaagcg gggatcagat cacctataaa attgacctgc ctgctcaaac
     2521 caaagtcgag caaatgaagt gcaacagttg cggcggcgca ttgaaagccg acaatgtcaa
     2581 gatgatcgcg ggcgcaccca ctgtggaatg tccatactgc ggcacggttt atcaattgac
     2641 cgaagagccc aagtggtaac ccttgcaatt ggagaagcca atatgaaaag aaaactattg
     2701 ctgctttcgt tcattttgat tctggtgctt gctgcatttt caaatgcctc tgcacaggat
     2761 gcgccgtatt acttcgaagt gaacaaggaa gtggttcacg tttactggaa ccctgacggc
     2821 acaatgagcg tggattacac ctggtatttc aacaacctgc caggctcaca tccgattgat
     2881 tttgtagatg tgggcatgcc caactacaac ttcaatacat ccaccattgt ggctgaatct
     2941 gacggcatgg atttgatggt cacgacgcag gactatcaag gctcagggtc gggttttgca
     3001 gtagtgatgg ggtcacgaac cattcagccc ggggataagg gcatactgca tgtcttcgtc
     3061 ccgaatattt caagcgtttt atacaacgat gcaaacgacg ccaactacgc cagcgccgtc
     3121 tttgcgccga catggtttgg tcaggaattc gtttcaggca acaccgattt caccatgacc
     3181 ttccacctgc cgccgaacat gaatccagaa gaaccacgct ggcacgaagc gccaagcgat
     3241 tttcctgaaa caccccaggc ggcctacgac tcggaaaacc gagttactta cacatggtat
     3301 gcacctgacg ccactgcgta caactactat tcttttggtg catcgtttcc acgtatttat
     3361 gtccctgcgg aggccattta caccccgccg ccagagccgc tcttcaatat tgatgaagac
     3421 accattttca gcctgctatg cggtggattt ttcttcttca tcttctttat ctcacctgcc
     3481 atcgcagcca tcacggggcg ccgccgcaaa atgcagtacc tgccgccgcg tgtggcaatt
     3541 gaaggtcacg gcatcaaacg cggactcaca gcggtggaag ctgccattct catggaacag
     3601 ccgcttgata aagtgatgac catgatcctg ttcagcgtgg tcaaaaaggg tgccgcacaa
     3661 gtggaaaccc gcgacccgct caaactttca gtggcggcag agcaacccgc caccctgcat
     3721 ccctacgaaa aagactttat caaagcattc gccgtggatg aaaaacgcag ccgcgttctg
     3781 caagatatga ccgttgcgct tatcaaaagc gtttccgaaa agatgcgcgg gtttagccgc
     3841 aaagaaaccg tcgagtacta caaatccatc acagagaaag cctgggctca agtggaagcg
     3901 gctggcacac cagaggtcaa gagccagaaa atggacgaat ctctggaatg gaccatgctt
     3961 gataaagatt acgatgaccg cgcacgccgt gtctacaatg gacctgtgta cgtaccaacg
     4021 tggtggggcc gttacgaccc gtcctatcgc cgcatatcca caccgacaac tgtttcgggg
     4081 ccgacgatat catccacccc gcgtccttcc tccagcggac aagccctgcc cggtgcagat
     4141 ttcgccgcat cgatggtgac aggtgtacag accttctcgc aaaaggtgat cggaaatgtt
     4201 cagtccttta ccgaacgcgt cactggggcg accaacccct tgcccaagag ttcctccagc
     4261 ggcggtcgca gcggcggagg cgggcgctcc tgtgcctgcg cctgtgcctg tgctggttgt
     4321 gcctgcgcct gcgctggcgg cggacggtaa acctcggcat gcttggcgct attttcaaaa
     4381 aagcaccgca cactcctccg catttatttg taccgaatgc gctctacatg ctcaccgcct
     4441 ctacctaccg cagtgagcca ttcatgcaaa caacagagcg caagttgaat tggatcaaaa
     4501 cctttctaac ttcagctgaa atgtacaaat gggaagtggt tgcctgggtg gtgttagaca
     4561 atcattacca cgccgtgctc aaagcgcccg actctgcaga caacctgccg aaatttgtgg
     4621 ccagctacca tcgcttcacg gcgcgaaact ggaatgaaga agaacaggca agcggaagga
     4681 aggtctggtg gaattattgg gatacctgca ttcgctcaga atacgattac tacaaccgcc
     4741 tgcgctacac cttttggaac cctgtcatgc acggccttgt aaaagacccg aacgattacc
     4801 catttagcag ctacaaggta tttctctcga aatggaaagt gaaattcaac tttacaaata
     4861 tggaagaggt gaaaaatgtc cctgaatttt agctccctga aaaacatcct gtccctgcca
     4921 tttcagcaag caccaaaggc cgatgcagcc gagaccgaaa cacggctcta tcactacgtc
     4981 catgaaaacg aaatggaaaa gtcgcgcatt cacttgcgca ttgaccccga cgggacgggg
     5041 acactcatcg tcaacgccag cagtgtcatg cacttaaatc caaccgcggc cttcatggcc
     5101 tggttgatcc tggaaggcaa aacggaagac gaaagggttc aagccctccg cgcccgttac
     5161 tcggtttcca aaaaacaggc ccgcgaagac cttgcagagt tcaattacca actcgaagaa
     5221 atgattcggc ctgacggcgc ctgcccagtg catgaacttg acctggaaac caaaatgcct
     5281 tttagcgcgc ggccttccgc cccctatcgc atggatctgg cgctcaccta tcgctgcaac
     5341 aacgactgcg cccattgcta caacgcacgt gaaagaaact tccccgagct caccacccag
     5401 caatggttca agatcatcga tcaactttgg gaattgggtg tgccgcacat cgttttcaca
     5461 ggcggcgaag ccacactgcg caacgacctg ccagagctga ttcatcatgc cgaaagcaac
     5521 ggtcagatca caggtctaaa cacaaacgcg cgccgtctga tggatatgga ctatgtcaaa
     5581 aaattggtca atgcagggct tgaccatgtc cagatcacgg tcgaatccag cgtgcctgaa
     5641 attcatgatg aaatgatgcg ggccaaaggc gcattccgcc aaaccattgc tggcctgaac
     5701 aatgtccttg cctccgaatt gtatgtgatg accaacacca ccatgcttcg caccaacgtc
     5761 cacacgattc ctgccacgct ggattttctc tccgacctgg gagtccccac catcggtctc
     5821 aacgccctca tctattcggg tcatggcctg accgttggga cgggtctgct cgaaagcgaa
     5881 ctgcaaccca tcctcaacat cgccacccaa aaaaccgccg agcatggaca aaaactaatt
     5941 tggtacaccc ccactcaata ctgccaattt gaccccactg cgcacaacct cggcgtaaaa
     6001 ggctgcacgg ccgcactgta cagtatgtgc atcgaatcga acggcaatgt ccttccctgt
     6061 caatcctatt accatccgct tggcaacatg ctaaccgact cgtgggattc gatctggaac
     6121 cacaaactct ccactcaatt gcgcgaaaga caaaacctgc ccttcaaatg cgagacctgc
     6181 tcactcgtcg cggaatgcgg cggcggatgc ccgcttcaat ttgaaaacca tcgtgaaaaa
     6241 tttggagtga atctgcctgt actgaccttg tagccgaaag ggtgaagagg acattctctt
     6301 cacccttcat cccttcccct ggagtctcat gaacgaccac gcctggatca aagaattccc
     6361 tgccgccatc accgtctgtg atgacgaggg catcatcctt gagatgaacg acaaagccgc
     6421 caaaaccttt gaaaaagacg gcggatacaa actcgtcggg tcgaatgtgt tggattgtca
     6481 caacgaccca tcacggggta aacttgaacg gttgatgtca gcccgcgaga aaaacgtcta
     6541 caccatcgaa aagaacgggg tcaaaaagat gatctttcaa tctccatggt ataaagatgg
     6601 catctatgcg ggcttcgtgg aactctctct ggagattcca ttcgaaatgc cacacttcat
     6661 ccgttcctaa cacctttacc tctcatggaa atctgctacc tgatcgcctt ccccgacaca
     6721 gacgatggtc aaaaccccgc ccctgagcgc ttcaaggggc tgaaagatgc gccgtatttt
     6781 caacccatcg acattgacct tgtcaccctc ggcgaagaaa ccctcatcat cgaaggccac
     6841 gccgtacagg ttttccgtca gcgctatgat ggttgtgtgc aaatgatcga atgtcacttc
     6901 gacctgaaag acccatttgc cgcctctgtc ctgcaggaac gtgccaagat ccaaagcgcc
     6961 cttgaaagca aattcgtccc cacccattac cgccaaaacg gactcttcga agagtacacc
     7021 attctgctcg taaaagaagc caagcccacc cccgataaat ggatcgaaaa gaacgctctg
     7081 gccctcacca aattcatccg ctcacaacgc gaagtgttcg acaaaagcga gatcgaagag
     7141 atcctgatct cgcgcacaca ttactcagcc gtggaactca ccctgataga ttgggaaggc
     7201 gccatcatca tcgcccccaa cgaagattac caatcggaca ttgccttgtt gaaaatcggc
     7261 aactaccagc tcctgcgcta ccgcatgctc gatcagtcca ttgaaaacac gctcgataag
     7321 atcaacgaaa cattcttcga aaagaaacgc ggattacgcc caacccgtgg cgtcatccgc
     7381 cagatcgcag aacacaaact cgaagtcatg ctcgatttcg aacgcgccga acaaaacctg
     7441 ctcctcatcg gtgattggta caccgccaaa ctgtatgaag ccattcaaag cgagttctac
     7501 ctcaaagact ggaaagaaaa cgtaaaaaca aaactcgata acctcgagaa catcgtccaa
     7561 accatcaggg ataatttctc cctatcgctt gaaagcgtat ggggccgcat tgaaatgctc
     7621 ggctggatga tcatgctcat cgggtacgcc tacctcttcc tcgcagatat gggcatcctc
     7681 aagatcggaa aataaacaca cggaacagga gttgacatga acttcattca aaagatcttc
     7741 accaaaaccg cgccgctccc cgcgggaact caccacatgc aagccatgca cgacgaaaag
     7801 ccctaccgcc tgcatttgcg ccttcacaaa gatggctctg gcctgctcat ccttaacgca
     7861 gccactgtca tccagctcaa ctcaaccgcc gccgaatacg cctatcactt catcaaaggc
     7921 acctcccccg atgaagccgc aaaacaggtt gccgcccgct accgtgtgga caaaaagacc
     7981 gcgctggcag atttcaacaa ctttgccgac cagatccaaa cgcttatctc aacgcctgac
     8041 cttgaccccg cctcctttct taactttgaa cgggcacagc cccattcaac agaatcaacc
     8101 ctcaggcttg attgcgccat cacctaccga ctgcccgaat ccactcacgc cacctacgct
     8161 cccgtcaaac gtgtagaccg tgaacttacc accacagagt ggcagtccat tctcgacaaa
     8221 gcctggcagg caggcattcc gcacatcatc ttcacaggcg gagaagccac cttgcgcgaa
     8281 gacctcccgc aactcatcgg ccacgctgaa aagaacgggc aagtctgcgg cctgctgacc
     8341 gatggtttga aactcgccga taaaaactat ttcgaaaccc ttctgcaaac aggcctcgac
     8401 cacgtcatgc tcatcctgca gcctgacgaa cccaaatcat ggaaagccat cgaaaccatc
     8461 gtcccgcagg atattttcct caccgttcat ttgaccctca ccaaagaaaa catcaacgag
     8521 gcccaaaaca ccctgcaaaa gctcgcctat ctcgatgtgc aaaacatttc actcagtgcc
     8581 gcagatgcca gtctgctcga tgaaatgctt gaacttcaag atacagccag ttccctccgc
     8641 ctcaccctac gctgggacct gcccgtgccc tactctgcgg ccaatcccgt ctccatcgaa
     8701 accgtggatg aagaaactcc ctccggtgca ggcaagacct ggatgtacgt cgaacccgat
     8761 ggcgatgttc tgcccgctca gggcgaaccc gataaaatcc tcggtaactt tttgagagat
     8821 gactggcgta caatttcaaa acaataggag cgcaaaagtg aaaatcatcg gcacagaaaa
     8881 tatgggcagc atggagctac aaatggaact tcaaaagggc ggaaagttca tcatgtacga
     8941 gtatgtcatt tccctgctcg tcatcacgtt caagcgcaac tccaacatct actttgtcaa
     9001 agcagatgaa agtgccgtag taaagggtct gccattcacc ttgctctcat tactcgtcgg
     9061 ttggtggggc attccctggg gaattatcta caccattcaa gcgatcttca ccaaccttag
     9121 cggcggaaaa gatgtgacaa ccgatgtact ctccgcaatc cgcgccagac aatctgcaca
     9181 aaacgtcaac ccagcctaaa atccagcagg gacattaaaa aggtaaccca tgaaaaaaaa
     9241 cttcgtggca gttttagcgt tgctcctctt ctctcttgca tgccaaaccc tcttcccaaa
     9301 gaatacaaca ccccccgata acaacgtcac cgctgaaccc acacaacgtg gaaccccatc
     9361 tcccatcgtt tgcaccgacg actcctgcat caacgcatgt cttgaaagga tcaacaacac
     9421 cctcgaaacc cgccgcttcg actcaatcgg cggggattac gccggtgcag aggcgaattt
     9481 tgaccttgtc aaatatcagg tggaaaacgg ggccatcagt gaccccgaaa tactctatgt
     9541 acccaaagaa ttcaaaccct ttcaagacga cctgcaagcc caacaaacgg tctggaaata
     9601 tgcatcatcc cttcttacag aaaacgaact caaatggatc actgagtacg tagtttttac
     9661 cgacggtgaa agtaacaccc tggcatgggt caacaataaa gacgatcttg atctttcgcg
     9721 ctggcagcta ggggtggata tcatagacgc cgccgatcct gtagacctga ccgccaccct
     9781 gatacatgaa ttcgggcacc tcatcaccct aaactcggaa caattcacac ccaccgacta
     9841 ttactacggc tggaatcaaa ataattccgc ctgcccgcaa ttcaccagcc cggaagggtg
     9901 cagcaccccc gattcctata tcaacctgtt ctatcaaaac ttctggatcg atatatttga
     9961 cgaatggctc gaaactgtcg aaaatcctca agtagactca gatgaagaat tcgacgcctt
    10021 acgaagagcg ttttactcaa agcacagcga acaatttgtc cgcgaatacg ccgccaccaa
    10081 catccgtgaa gatatggccg agtcgttcat gcattttgtc ctcacccccc aacccaatgg
    10141 aaacaatccc gtggaacaaa aaatgctctt cttttaccaa tttgaagaac tgaccaccct
    10201 gcgccgccag atcatccaaa acgtctgttc atacactcag gaataaaatg accaaactcc
    10261 ccatccccga atgttactgg gtcgaagaaa accgccttct ggcaggtgaa tacccgggcc
    10321 atcacgaccc agagatcatg cgtcgcagaa tcgatgcctt tctcgaagcg ggcgcggata
    10381 cctttttcga cctcacacaa gcgcacgaac tcgtcccata cgaagccacc ctgcaggaag
    10441 aagcacgcat atacggcata gacgcctcct atcaacgctt tcccatccgc gattatggca
    10501 ttcccactgc aaacaccatg accgccatcc tcaatgccat agacgaagct acaagcgcag
    10561 gtcactgcgt gtatgtccat tgctggggcg gcgtgggacg caccgggctc gtcgttggct
    10621 gccaccttgt ccgccacggc ttcacaaacc aacaagccat agctcaggtg gacacactgt
    10681 tcaaaacccg cccaaaccgc atcttccatc cccgctcgcc agaaagcgac gaacaagtgg
    10741 acttcatcct caattggtgg gaagacgcaa ccgaactcca ccgaaataac aaaagattct
    10801 gcgaaggatg aactggcaca cgcgctacac gcaacaagca aactggaccc gagaattgag
    10861 ggcctatctt tttgaacagg ctgggatgga tcgtgccaaa cggattctcg aagtaggctg
    10921 cggtacgggt gccattttga gcaccctcaa cacctcagcc acgcgtcatg gtctggacct
    10981 tgagcctgct tcgctcgccg agtgcagaat ccacgccccg gcagccttgc taagctgtgc
    11041 cgatgcacac tctctcccct accccgatca gtcgttcgat attgtctatt gtcacttcct
    11101 gcttttgtgg gtgaaagatc cattgcaggt tgttcgcgaa atggctcgcg tgggccgcac
    11161 cgtgttagca ttggccgaac ctgattacag tcaacgtgtt gacgaacctg ccacgctcaa
    11221 atcactgggc gtatggcaga cagagtcatt acgccgacag ggagctgacc catcattcgg
    11281 cggaatgctg gccgaaacat tccatcaggc tggcatcaaa ttgatcgaaa caggacccat
    11341 tcaaagtcag gttgtgatgc gttctgccga agattgggaa actgaatggg ccgtgctcca
    11401 aacagatttg gaaggtatcg cccaaaggga agaaatccaa aaaatgaagg ttttggatga
    11461 aattgcgcga aaaaatggag ataggacctt gtatgtgcct acatactttg cctggggtaa
    11521 gacttaagga gtggcagact tcgaataaag ggaagtataa ttttgcccat acattggagg
    11581 catgattgga aacattacaa cacgaagaac aaccccaaag cccaattgag gggcaggttg
    11641 agggtcaaat agaagggcag cctacacagg aagcgcctgc tcaagaaaag ataaagtggg
    11701 gccgcttttt tgtggatatt ctggaaactt tggtgctggc cgttatttta tttttcggta
    11761 tcaatgcggt ttcagcgcgc gtacgggtgg atgggtatag catgcgcccc acccttgatg
    11821 acggtgaatt tgttctggtt agcaaattca attacaaatt aagcaatgtg cagcgcggcg
    11881 atattattgt gttccacttc ccgatggacc ctaaacagga gttgatcaaa agagtgatcg
    11941 gcctgccggg cgatcgggtt cacgtgaaag atggcgttgt ttacataaac aaccaggcat
    12001 tggacgaacc ctatattgcc gcccctcccg cttataccag catgtggaat gtccctgaag
    12061 atcgtctgtt cgttttgggc gataatcgca atgattcatc tgattcacat tcctggggtt
    12121 atttgccctt tgaaaatgtg gttggcaagg cggttttgat ctattggccg cccccaatgt
    12181 gggacgtgat cgaacacaag gacttactgg ttgcacaata aggatgcccc atgagtgaca
    12241 acgaatacac accacggtca ttcccctgta acgaatgtca ttctggcatt ctacagccgc
    12301 gtcacatcac gtattttaca tggcttggca atgagttgat cacggtgccg catttccccg
    12361 cctgggtttg tgatgtgtgt ggaaaacgcg agtacgatga aaaagcaatc agctggctga
    12421 acatgatcct cgaccccaat gcaggcaagc cgaatgcaaa atcgcgccgc ccgttaccgc
    12481 gccccaaact ccgtacgaac aatccccgtc ctgcccacga ttcgtaaccg agagggaatt
    12541 ttcactccat gacacttgat acttctccgc gcacctatcg ttttcttcca gcagacatca
    12601 tgacttcagg gtatcgtgtg gtcggcaaga ttatgatcac aaacaacggt gtcatgggga
    12661 tgatgaatga cgcaactcat tctgccatgg aagtgaatga tgcgcggctg gctcgtttgc
    12721 atatgccaac gaagcttgtg gaccattttg aattaattcg catgatgaag cgtcagatcc
    12781 atgctgtgtg tctggcccgc cgtgaagacc tgggtccact ggctctggtg cgcggcggat
    12841 atgccagcgt ggtggagcac ccggtacgga tcgcaactca aatgttcgaa gtggagggaa
    12901 ccatggaatt accaggacgc ttcgacttca ctgccattat gacagatggc acgcgaactt
    12961 ttttgcctgt gctgaacgct acactgactg caatcctcat tccaaacctg cgtgtcgaga
    13021 gtgccagcat ccttgtcaac cgcaatttga tcgacattat ggcgctgatt aatcaacggg
    13081 tgaaaccaga aattcaaccc taaaatcgct tgactgacac agaatgggat gatagaattc
    13141 gcccgctcta aaaaaggccc ccatcgtcta gaggcctagg acgcggccct ttcaaggcca
    13201 cgaccggggt tcgaatcccc gtgggggcac
//
LOCUS       DAOQLC010000118        13185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1766_length_13185_cov_23.707388, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000118 DAOQLC010000000
VERSION     DAOQLC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1766_length_13185_cov_23.707388"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1046)
                     /locus_tag="PL000_16985"
     CDS             complement(198..1046)
                     /locus_tag="PL000_16985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC90626.1"
                     /translation="MDSLSERRKAILFLALAAILWSTSGLFIKILDWQPLSILAARSF
                     FAAIVFLVYLRRIPMRFNLWQLLAAGMFILTQFLFITSTKLTTAANAIFLQYTAPIYV
                     VLLAFWFLHEKPSRVDWLSMLIIFIGLTLFFGDKLSTEGFYGNLLAILSGVTSAVMMV
                     SFRAQKDGNPAESNLIAFLITAAFGFPFVLKETWTVESWSILAFLGVFQIGFAFIFFT
                     QGIKHIPALEANLIGTLEPVLNPIWVFLFYGESMGAYALIGGLVVLGGVVLSAVGSAK
                     AVKDRH"
     gene            complement(1170..2381)
                     /locus_tag="PL000_16990"
     CDS             complement(1170..2381)
                     /locus_tag="PL000_16990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC90627.1"
                     /translation="MTDAPSPLIFISYSRVDSKAQQRLADALRKHGLTVWVDNEKLTP
                     GNPAWEREIEKAILSCSAVIAILSPEAKRSEWVRREISFAEQHNKRIFPVLLRGDESS
                     TMTLRMINHQFVDLRQNEKEGVEVLFNTISEYLEYVKANPSGEGAGSNSLFSKPKAWI
                     FAVLGIFALLMTTSFLLKMGPFTVPATPTATTPPTATATIPPTATAQPEVVIPEFFTV
                     DFDTDLENWDTFLVGDPENEVKTSVEKSYMKFELSGKNQHYYTYYTLRTYNNVRVDIR
                     SENRGEYNSVATVICRYHIDKDWYEFNFDSGGLYQILYVKWNDDKTKTISTLIANGGS
                     SLINAGKNVNDYSVICNDRTLSLWINGNEISTVIDNRFVLEEGYVGIGVSSGTLIPIE
                     NRIDTLTISQP"
     gene            complement(2556..3503)
                     /locus_tag="PL000_16995"
     CDS             complement(2556..3503)
                     /locus_tag="PL000_16995"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HNC90628.1"
                     /translation="MYHPTTRVLAVLSLLQTYGRMSGAELAQRLEVNIRTLRRYIVML
                     QDLGIPIEAERGRNGAYVLSAGFKLPPMMFTNEEALALTVGLISVHRLNPADTGYAVE
                     SALAKLERVMPLDLKSRVRALTETITLDLKTESSVPTNEVILSALSSAAQLQKQVHIQ
                     YRPDQGEETERDFDPYGLTYFQNKWYVIGFCHLRESLRSFRLDRISGIKTLEAGFSRP
                     DGFDALGHIMQAIATLPRKYPFEIVLKTDIETAQREVFDVLGVLEAGRDGIILRGSVD
                     DLDWLARQLSIFSFGFSVRTPKELKTALKQHAVRLGNVI"
     gene            complement(3523..4245)
                     /locus_tag="PL000_17000"
     CDS             complement(3523..4245)
                     /locus_tag="PL000_17000"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HNC90629.1"
                     /translation="MSRQTHSPIVELRMYKLRKNRRDELIELFEREFIESREAVGIQV
                     LGQFRNLDDPQSFVWLQGFSDMPARAQALHAFYTSPIWMENRKAANATILDSGNVLLL
                     HPARPDSGFKLKIGARPPRGNTTPQDGFVAVTICYFDGSVTNEFIEYFESTLQPALKE
                     AGASILAYFVTEDSPNTYPRLAIREGEHVFVWFAGFRESETFAAMLKSKAWKEISKSL
                     SQHVSGDPEVLRLTPTPRSWLK"
     gene            complement(4242..4718)
                     /locus_tag="PL000_17005"
     CDS             complement(4242..4718)
                     /locus_tag="PL000_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90630.1"
                     /translation="MQTENDGRNDFNFFIGNWTVKHRKLKELLKNCAEWDEFESTVVD
                     RAIMGGIGNLEEMIFERTAGRFYGTSLSIFNPKTKQWSQYWVDSANATLQDPMIGKFT
                     DGVGEFFSEDEFEGRTVLARARWYDIEENSCKWEQSLSDDGGQTWETNWVMEFIRA"
     gene            complement(4745..5209)
                     /locus_tag="PL000_17010"
     CDS             complement(4745..5209)
                     /locus_tag="PL000_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90631.1"
                     /translation="MDGRNGFDFLVGSWKVHHRRLRERLKGSSDWEEFKGDTVDRKIL
                     NGLGNMDENIMYRETGPIHAVTLRLFDPQAQEWSLYWATGISGILEVPMIGGFKHGLG
                     EFYSQEVFEGRHIFSRFVWSEITTVSCKWEQAFSADGGQTWETNWVMDFERV"
     gene            5427..5963
                     /locus_tag="PL000_17015"
     CDS             5427..5963
                     /locus_tag="PL000_17015"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HNC90632.1"
                     /translation="MTNRYKIRQNLISIGDDFWIENEEGKKVFKIDGKVLRIRKTLVF
                     EDAKGKKLAQIQERLLTIKDTMVVEDADGKDIATIKKALISPFRDKWDVNVKNGPDLD
                     VQGNILDHEYTVKQGREKVAEISKKWFRLTDTYGVEIEDGQNDILILAIAVAVDMMAH
                     DDKEDKKRKKEKDEKKDR"
     gene            complement(6023..6766)
                     /locus_tag="PL000_17020"
     CDS             complement(6023..6766)
                     /locus_tag="PL000_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90633.1"
                     /translation="MKKSLDVKKTAIIFFVLLFAAYIPVFLKEQVLDAFVREDRIYET
                     LSPIYLFVTSSMFAVAFYRSPIKLSLKDPAWLKRLFFLAFCGLFFLAAMEEISWGQRI
                     FGVETPNLIKDVNVQEELTFHNLNVFQGDDAKLPLDFDQLSAIFALTFGFLVPFVCYF
                     FIPVKNFLGTKFPVLPLQIGLLYPVNYLIQKTLMRVLPIFPSLFQHPNMRFPQAVYEV
                     REHNYAMLLMVSAVFFILLKLDLAGDGTQ"
     gene            complement(7082..7516)
                     /locus_tag="PL000_17025"
     CDS             complement(7082..7516)
                     /locus_tag="PL000_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC90634.1"
                     /translation="MYKKILVPLDGSPLAEAVLPHAQALAKSEGAEIVLLSVPVTPSY
                     DYLERTPGLASQIIESAERETEAYLKAEVEKLTGEGMKVSGVMREGPIPEMILKVAEE
                     VHADMIAMSTHGRSGIQRWLMGSVADRVVHHAHIPVMLIHPN"
     gene            7630..8514
                     /locus_tag="PL000_17030"
     CDS             7630..8514
                     /locus_tag="PL000_17030"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNC90635.1"
                     /translation="MPMALYLGCPVWSFKGWVGNFYPKGTKSTDFLREYARRLTTIEG
                     NTTFYAVPAPKTIEAWAEQTPETFRFCPKIPKAISHNGKLADYADRARQFVEIMRPLA
                     SRLGPMFLQLPPSYSPRLMDDLATFLDAFPTDIRLGVEVRHLDWFEEPHRTRLNQLLT
                     DHNMARVVIDTRPIRDLAGDESIKGSSYESLLEARERKPNVPVFEEQTTDFTFLRYIG
                     HPQMEQNQAWVEEWVNRITDQLSAGTDAFVFCHSPDNMLAPSICREIHQKISSRIKIQ
                     PLPWDDIRSDVFEQGKLF"
     gene            complement(8511..8885)
                     /locus_tag="PL000_17035"
     CDS             complement(8511..8885)
                     /locus_tag="PL000_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC90636.1"
                     /translation="MNSKQVIETFWQMMEGNDFYAVAQLLHEEYTLDWPQSGERIRGA
                     ENFAKLNTAYPAEGKWRFVINSIVVDGENVVTDVSVTDGVRHDRVITFSTIRDEKIWK
                     QVEFWPEPFEAPNWRAQWVERI"
     gene            complement(8887..9282)
                     /locus_tag="PL000_17040"
     CDS             complement(8887..9282)
                     /locus_tag="PL000_17040"
                     /inference="COORDINATES: protein motif:HMM:NF021514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC90637.1"
                     /translation="MSGETELIKLLQNMKPELNTGEYVYCRADSKKQAAELEPLCFFI
                     EKEGVTMILPKEKAKAMNIPYTTVCAWITLTVHSSLEAVGLTAAVSKVLTEANISCNV
                     VAAFYHDHIFVPIADASRAMDALLSLTKG"
     gene            complement(9279..10274)
                     /locus_tag="PL000_17045"
     CDS             complement(9279..10274)
                     /locus_tag="PL000_17045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90638.1"
                     /translation="MTNIPLTPLLEKILASRSAWIDSKHESAFRLFNGFLEGCPELAI
                     DVYASTAVIHNYADDFEHGQALVEEASRFLRASLNWLRAGIVKTRHGTQEEKRGTLLF
                     GTEFDRKIKEHDTWYAINPILNRDASFYLDTRNLRKWLIENAKDKMILNTFAYTGSFG
                     VAALKGGANRVVQIDLNREFLNLAKSSYTLNGFPIRKGDFIARDFFEQVSSMKRTNTF
                     FDYVLIDPPFFSSTSKGRVDQVHESARLINKVRPLINDGGSLIAINNALFVSGDEYMQ
                     SLEALCKDGYLKINELIPIPEDFTGYPETRQGNPVTNPAPFNHSTKIAILGVKRK"
     gene            10415..10999
                     /locus_tag="PL000_17050"
     CDS             10415..10999
                     /locus_tag="PL000_17050"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC90639.1"
                     /translation="MQQRSEETRSKILGAAIKLFSTRGFNSASVDDICRDAGISKGAF
                     YHHFKSKQALFLALLDGWLQTIDNAIEASKDKTVPETFMQITEAFPYIFETAGEGLPM
                     FLEFWMQASRDEKIWQASIAPYRRYQKHFTQLIKKGVDEGSFVEVDPELTSRMIISTA
                     MGLLLQSLLDPKGAKWEKVARDSTNMLVNSLLKK"
     gene            11096..11959
                     /locus_tag="PL000_17055"
     CDS             11096..11959
                     /locus_tag="PL000_17055"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNC90640.1"
                     /translation="MKIVTDCAADMPNEELERLGVVQAPLFIQFPEGEVNAMEISADE
                     FYNRLEAMRPQIPTTAQPSSGIFAELYRKLAQTEKNIFSVHISSGLSGTINSARDGGE
                     QVKAEANVSFWDTLTLSGGERFQVVAAALAHKAGWAMDKIQERLMKIREKTEVIYTLD
                     TLEYLARGGRIGRVKALAGALLNLKPVIRVDTDGKYSTVKTERTINKSIATIADHLVE
                     KYGNTPVWVTVLHGRFAEKADLLADEFRQKLNVAKMEVQRISPVLGVHTGPGIVGAAV
                     VPMELMNDLGE"
     gene            complement(12019..>13185)
                     /locus_tag="PL000_17060"
     CDS             complement(12019..>13185)
                     /locus_tag="PL000_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC90641.1"
                     /translation="FNLTLPMTALDLEKQKQAKEYSRINRRLWLVDTLFGVAYALAWL
                     FLGWSISLREWLHQLVTVQWSVTTGADWLIIALYVAVFGGISAIINLPLSYYSGFVLP
                     HRFGQSNQSLKDWLVDQVKGLAIGAPLGLIMLELMYLALRLTGDAWWLWVAGGLLLFN
                     ILVSNLAPVLIMPLFNKYVPLGEEHKELEERLLALAERANTKVKGVFKFDMSKRTKAA
                     NAALTGLGNTRRIILGDTLITEFTLDEIETVLAHELGHHVHKDIPFLIAFGTVSTTLS
                     LFIASLALDSAVSFFSFTSPADIAALPALMLILTAYGLLTSPVNNAISRWRENMADDY
                     ALSSTQKGEAFASAFTRLANQNLGEIDPEKWVVFMFYSHPPLGERIEKAKRFAA"
BASE COUNT         3566 a         3229 c         3224 g         3166 t
ORIGIN      
        1 gcgcgcccat cacccaatca aggtcgaggg gcgtgccggg gttacgagtg tgattactgg
       61 tcataatttg ccttttcatt cagccaaact aatattttcc atactataac ataaggtgaa
      121 ttcgagcgga gatttatcac aattgggcgt gcagcaaatt accaagagaa tggtcaaagg
      181 tataaggttg catgatttca atgcctgtct ttcactgcct ttgcactccc aaccgcgctc
      241 aaaacaaccc cgcccaatac caccaacccg ccgatcaaag cataagcgcc catgctctct
      301 ccataaaaca gaaacaccca aatgggattc aaaactggct ccagagtacc gatcaggttt
      361 gcctccaggg cagggatatg tttgatgccc tgtgtaaaaa atatgaaggc aaacccaatt
      421 tgaaatacgc caagaaaagc aaggatggac cagctttcca cggtccatgt ttcttttagc
      481 acgaagggaa agccaaatgc agccgtgatc aaaaatgcaa tgaggttgct ctcagcgggg
      541 ttgccatctt tttgagcacg gaaggaaacc atcatcaccg ccgaggtcac tccgctgagg
      601 atggcaagca ggttgccgta gaatccctct gtgctgagct tgtcaccgaa gaagagggtc
      661 agcccaataa agatgataag catcgaaagc caatccacac gggagggttt ttcgtgcagg
      721 aaccagaagg caagcaacac gacataaatg ggcgccgtgt attgcagaaa gatggcgttt
      781 gcagccgtgg tcaatttggt ggaagtgatg aacaggaact gtgtcagaat gaacatccct
      841 gccgccagca gctgccaaag attgaaacgc atcgggatgc ggcgcaggta aacgagaaaa
      901 acaatggcgg caaagaaact gcgggctgcc aggatcgaaa ggggctgcca atccaaaatt
      961 ttgatgaaca acccgcttgt gctccacaaa atggcggcaa gtgcaagaaa aagaatcgcc
     1021 tttcggcgct ctgaaagaga atccatcgaa ttatccttat aagaaatagg gttggttctg
     1081 aattatacgg catcgggaaa gttctgctcg aaacgcttaa aaacaaagag ccgcctttat
     1141 gacaggcggc tccttgaagg ggacacagat taaggctggc tgatggtgag cgtatcaatt
     1201 ctgttctcaa ttggaatcag cgtgccagaa gaaacaccga tccccacata gccttcctcc
     1261 aacacaaacc tgttatcaat cacagtgctg atctcgttgc cattgatcca cagcgaaaga
     1321 gtgcggtcgt tgcaaatcac cgaataatcg ttgacattct ttcctgcatt aataaggctc
     1381 gaaccgccat tggcgatcaa tgtggaaatg gtctttgtct tatcgtcgtt ccatttgacg
     1441 tacaaaatct gatacagacc gccgctgtcg aaattaaatt cgtaccagtc cttgtcaata
     1501 tgatagcggc agatcacagt ggcaacgctg ttgtattcac cgcggttttc gctgcggata
     1561 tcaacgcgga cattgttata agttctgagc gtgtaatagg tatagtagtg ttgatttttg
     1621 ccgctgagtt cgaatttcat atagcttttt tcaacgcttg tctttacttc gttctcagga
     1681 tcgccgacga gaaaggtatc ccagttctca agatcggtgt caaaatccac tgtgaagaac
     1741 tcagggatga ccacctcagg ctgggctgtg gcagtgggcg ggatggtggc ggtggccgtt
     1801 gggggtgttg tggctgttgg cgttgctgga acggtaaagg gccccatctt caggaggaat
     1861 gaagttgtca taagcagggc aaagatgccg agtacggcaa aaatccatgc ctttggtttt
     1921 gagaacaggg aattcgagcc cgcgccttca ccagaagggt tggccttcac atattccaga
     1981 tattctgaaa tcgtattgaa gagaacttcg accccttctt tttcattttg gcgcagatca
     2041 acgaattgat ggttgatcat acgcaatgtc atggtggaac tttcatcgcc gcgcagcaag
     2101 acagggaaaa tgcgtttgtt atgctgctcg gcaaagctga tctcacggcg cacccactcc
     2161 gaccgttttg cttcaggtga aagaatcgca attacagcac tgcaagacag gatcgctttc
     2221 tcgatctccc gctcccatgc agggtttcct ggagtaagtt tttcgttatc cacccacact
     2281 gtcagcccat gcttacgcaa ggcatctgcc aggcgctgtt gggctttcga gtccactcgg
     2341 ctgtaactta taaaaattaa tggggaaggc gcgtcggtca agttggtctc cgtaaaattc
     2401 gtctctttgc cctaccagac aggctgttct tattcataaa acaccgccga aaacggcggc
     2461 tgaagcctgt gtgaaatttt aaggcaaaga aaaattcgta ggcattataa gccaaaagac
     2521 cttactttga ctttgctatt atgttatgta gagaatcaga tcacatttcc cagcctgacg
     2581 gcgtgttgct tcaaggctgt tttcaattct ttgggtgtac gcacggaaaa gccaaaggaa
     2641 aaaatggata gttgcctggc gagccaatcg agatcatcca cactaccgcg caggatgata
     2701 ccatcgcggc ctgcctctaa cacacccagc acatcgaaca cctctcgctg tgcggtttca
     2761 atatctgttt ttagcacgat ctcaaatgga tattttcgcg gcagggtggc aatcgcctgc
     2821 atgatgtgac ccaacgcatc aaacccatcg ggacggctga atcctgcttc gagagttttg
     2881 attccgctga tacgatccag ccgaaatgaa cgcagacttt cgcgcaagtg acaaaagcca
     2941 attacatacc atttgttttg aaaataggtc agcccgtagg gatcaaaatc acgctcggtc
     3001 tcttcacctt gatcggggcg gtattgaatg tgtacctgtt tttgcaattg cgccgcactg
     3061 ctcaaggcgc tgaggatcac ctcattcgtc ggtacagagg attcagtttt gagatcgagc
     3121 gtgatggtct ccgtcaacgc gcggacacgg ctcttcaggt cgaggggcat cacccgctcc
     3181 agcttggcta atgcactttc gacagcgtac cctgtgtcgg cggggttgag gcgatgcaca
     3241 gagatgagac ccaccgtcag cgccagcgct tcctcgttcg tgaacatcat cggcggcagt
     3301 ttgaatcccg cagagagtac gtacgcgccg ttccttccgc gctccgcctc gatgggaatg
     3361 ccaaggtctt gcagcatcac aatatagcgc cgcaaagtgc ggatgttgac ctcaagccgt
     3421 tgggccaact ccgcaccaga catgcgcccg taggtctgca gcaaagacaa cacggccagc
     3481 acacgcgtag tggggtgata catacgcgcg agtatacacg ttttatttca gccacgaacg
     3541 cggggtggga gtgagccgca acacctcagg atcaccgctg acatgttgac tcaacgattt
     3601 ggatatctcc ttccaagcct ttgactttaa catcgccgcg aacgtttctg actcgcgaaa
     3661 gcctgcgaac caaacaaaaa catgttcgcc ctcgcgaatt gccagccttg gatatgtatt
     3721 cgggctatcc tcagtgacaa aataagccag aatcgatgcc cctgcttcct tcaatgcagg
     3781 ctgaagcgtg gactcaaaat attcaatgaa ttcattggtg acagacccat caaaataaca
     3841 gatcgtcact gctacaaatc catcttgcgg ggttgtattg ccccgtggtg gacgagcacc
     3901 tattttcaat ttgaatccag aatcggggcg ggcggggtgc agcaaaagta cattgcccga
     3961 gtcaagaatg gtggcattgg cagctttgcg gttctccatc caaatgggac tggtataaaa
     4021 cgcatggagt gcctgtgcgc gggcaggcat gtcgctgaat ccctgcagcc atacaaaact
     4081 ttgtggatca tccaaattgc gaaattgacc gagtacttga atacccaccg cttctcgaga
     4141 ctcaatgaat tcacgttcaa agagttcgat cagttcatcg cgtctgttct ttcgcaattt
     4201 gtacatgcgc aattcaacga ttggggaatg ggtctgcctg ctcatgcgcg gatgaactcc
     4261 ataacccaat tggtctccca ggtctggccg ccgtcatctg aaagagattg ctcccacttg
     4321 caggaattct cttcgatgtc ataccaacgt gcgcgagcaa gaacggttcg accttcaaac
     4381 tcatcttctg aaaagaactc acccacccca tcggtaaatt tcccgatcat cgggtcttgt
     4441 aaagtggcat tggcgctatc aacccaatac tgactccact gctttgtttt tgggttgaat
     4501 atgctcaggg aagtgccata gaaccgcccc gcggttcttt caaagatcat ttcttcaaga
     4561 ttaccaatgc cgcccatgat ggcacggtct acaacggtgc tttcgaattc atcccattcg
     4621 gcacaatttt tcaaaagttc tttgagtttg cggtgtttaa ccgtccaatt cccgatgaag
     4681 aaattaaaat cgttcctgcc atcattttct gtttgcatgt tcggctcctt gaaatatgtt
     4741 gaatttatac gcgctcgaaa tccatgaccc agttggtttc ccaggtctgg ccgccatcgg
     4801 ctgaaaatgc ctgttcccac ttgcaggaaa cggttgtgat ctcagaccaa acgaaacggc
     4861 tgaagatgtg acggccttca aagacttcct gcgaataaaa ttcgccaagt ccgtgcttga
     4921 agccgccgat catgggcacc tccaatatcc cgctgatgcc tgtggcccaa tacaaactcc
     4981 actcctgcgc ctggggatcg aacaaccgca aggtcacagc atggatcggc cccgtctcac
     5041 ggtacatgat gttctcgtcc atgttgccaa ggccgttcag gatctttcta tccacagtat
     5101 cgcccttgaa ttcctcccag tcagacgacc ctttcagccg ctctctcaac ctgcgatgat
     5161 gtaccttcca ggacccgacc agaaaatcaa aaccatttct tccatccatt atttatgctc
     5221 cttataaagt ggttagtaca atagcctcag tataggaaga aatgcggaca ggatcgttcc
     5281 taatttaaaa gggatttctt aataaaataa cccacgttca tcacatattt ttgcaatcca
     5341 atcggcatac agcatgaaca tgctgaaaaa tccaatcgag tatactcacc atgaaagttc
     5401 gttcatccgt aaaaagaggt gctcaaatga caaatcgtta caaaatccgc caaaacctga
     5461 tctccattgg agatgatttt tggatcgaaa acgaggaagg caaaaaggtc ttcaaaatag
     5521 acggcaaagt gttgcgcatc cgcaagacac tggtgtttga agatgcaaaa ggcaaaaagc
     5581 tcgctcaaat tcaggaacgg ttgttgacca tcaaagacac aatggtggtg gaagatgccg
     5641 atggcaaaga cattgccacc attaagaagg cgttgatttc ccccttccgt gataaatggg
     5701 atgtcaatgt aaagaacgga cccgatcttg atgtgcaggg gaacattctc gaccatgaat
     5761 atacggtcaa acaggggcgg gaaaaagtag ctgaaatttc aaagaaatgg ttccgcctga
     5821 ccgacacata cggcgtggaa atcgaagatg ggcaaaacga tattctcatt cttgccattg
     5881 ccgttgcagt ggatatgatg gcgcatgacg acaaggaaga taagaagcgc aaaaaggaaa
     5941 aggacgagaa aaaggacagg taaattcaaa aaaaatatgc cgcgattgcg gcatattttt
     6001 tttcttggat aaaggaaagt ttttattgag tcccatcgcc agcaagatcc agtttcaaca
     6061 aaataaagaa cactgctgaa accatcaaca gcatggcgta attatgttca cggacttcat
     6121 aaacggcctg cggaaatctc atattgggat gctgaaatag cgacgggaaa atcggcaaga
     6181 cccgcatcaa ggtcttttgg atcagataat tcacaggata taacaggccg atttgcagcg
     6241 gcaacacggg aaatttcgtt cccaaaaagt tttttacagg aataaaaaag taacaaacga
     6301 atggcaccag aaaaccaaag gtcagggcga agatcgcact caattggtca aaatccagcg
     6361 gaagtttggc gtcatctcct tgaaacacat tcaaattatg aaaagtcaat tcttcctgga
     6421 cgttcacatc tttgatcagg tttggggtct caaccccaaa aatcctctgc ccccagctga
     6481 tctcctccat ggcggcaaga aagaacaaac cgcaaaaagc aaggaagaaa agccgcttaa
     6541 gccaggcagg gtctttcaaa ctaagcttga tcggcgatcg atagaatgca actgcaaaca
     6601 tgctggaagt aacaaacagg taaattggag aaagggtttc ataaattcga tcctctcgaa
     6661 caaacgcatc caaaacctgt tccttcaaaa atacggggat gtaggcggca aacagcaata
     6721 caaaaaaaat gatcgctgtt ttcttgacat caagactttt tttcattcgt caccttattc
     6781 gttttaaagt tattgattat gcagatggca ggagaagggc atattcaaca gacttgctta
     6841 attgctatgt gagatcaaat tcatatcagc atccataaaa aatcaccttg cataaaactc
     6901 tcccattaac cgcggcaagt atagcattaa ttaaatttcg aacatcagaa ccccaaaaaa
     6961 accttggtac tattcaaaaa taatcacaaa aaaaaacaat gggcttttgg gggaaatacc
     7021 gcaaaaaccc attgacctca aatcaaaatt ctaatgaaat ctacacccct ttgtatttgc
     7081 cctagtttgg gtggatcaac atgacaggga tatgggcatg atgcacaacc cgatctgcca
     7141 cgctgcccat tagccagcgt tgaattccag agcgcccatg tgtagacatg gcgatcatat
     7201 cagcatggac ctcttcggcc accttcaaga tcatttcggg aattggcccc tcacgcatca
     7261 caccgctgac cttcatcccc tcccctgtca gcttttccac ttcagccttg agatacgcct
     7321 cggtttccct ttcggcactt tcgatgatct ggctggccag accaggtgtg cgttcgagat
     7381 aatcatagct tggggtgact ggcacactca aaagcacgat ctccgcgcct tctgattttg
     7441 ccagagcctg cgcatggggc aacactgcct ccgcaagcgg cgagccatcc aatggaacga
     7501 ggatcttttt gtacatggta catctccttt atctgaaaag aaaatctttt gaataagctc
     7561 atctatacca tagcacggcg gggggaattt tcaagtgaca atagacacat cttctgcaaa
     7621 gcatataata tgcccatggc tctatatctt ggctgtcccg tctggtcttt caaaggctgg
     7681 gtcggaaatt tttaccccaa aggcacaaag tccactgact ttttgcgcga atatgcccgc
     7741 cgcctgacaa ccatcgaagg caacacaact ttctacgctg tgcctgcgcc aaaaacaatt
     7801 gaagcatggg cagagcaaac gccagagacc ttccgctttt gtccgaagat tccaaaggcg
     7861 atcagtcata acggaaaact ggcagattat gccgaccgcg cccgccaatt tgtggaaatc
     7921 atgcgcccgc tcgcttctcg tcttggaccg atgttcctgc aactgccgcc cagttattcg
     7981 ccgcgtctga tggatgacct tgccacattc ctggatgcct ttccaacaga tatacgcctt
     8041 ggcgtggaag tacgccacct cgactggttc gaggagccgc atcgtacaag gctgaaccaa
     8101 ttgttgaccg atcacaatat ggcacgggtt gtcattgaca cgcgcccgat tcgagatctg
     8161 gctggcgatg aatccatcaa gggaagttca tatgaaagtc tgctcgaagc acgggagcgc
     8221 aaaccaaatg tccccgtctt tgaagagcaa accacagatt tcacttttct gcggtacatt
     8281 ggtcacccac aaatggagca gaaccaagcc tgggtggagg aatgggtcaa tcgaatcaca
     8341 gaccaattgt cggcggggac agatgcgttc gtcttctgtc attcacctga caatatgctc
     8401 gcaccgtcca tctgccgcga aattcaccag aaaatttcga gccgaatcaa aattcagccg
     8461 ctgccgtggg atgatatccg ctcggatgtt tttgaacagg gaaaattatt ctaaattcgc
     8521 tccacccatt gcgcacgcca gttgggggct tcaaatggct cgggccagaa ttcaacctgt
     8581 ttccatatct tctcatcacg gatggtggaa aatgtgatga cgcgatcgtg ccttacccca
     8641 tcagtgacgg agacatcggt tacgacattt tcaccatcga caacaatgga gttgatgacg
     8701 aagcgccact tgccttccgc aggataggcg gtgttgagtt tggcaaaatt ttccgcgccg
     8761 cgaatgcgtt cacccgattg aggccagtcg agggtgtatt cttcgtgcaa tagttgggca
     8821 acggcataaa aatcattgcc ctccatcatc tgccagaatg tttcgatcac ttgctttgaa
     8881 ttcatgtcaa cctttggtga gggaaagcaa ggcatccatc gcacgcgagg catctgcgat
     8941 cgggacaaag atatggtcgt gataaaacgc agcaaccaca ttgcagctaa tattggcctc
     9001 tgtcagaacc tttgagactg ccgcggttaa gccgaccgct tcaagcgaag agtggacggt
     9061 aagtgtgatc cacgcacaga cggttgtgta ggggatgttc atcgccttgg ctttttcttt
     9121 tggcaggatc atcgtcacac cttctttttc aatgaaaaaa caaagtggct ctaattctgc
     9181 ggcctgcttc tttgaatcgg cacggcaata tacgtattcg cccgtgttga gttcgggttt
     9241 catgttttgc aaaagtttga tgagctcggt ttctccgctc attttcgttt gacacctaaa
     9301 atcgcgatct tggtggaatg attgaatggc gcagggttgg taacggggtt gccctgtctg
     9361 gtttcggggt aacctgtaaa atcttcagga atggggatga gctcgttgat cttcaaatac
     9421 ccatctttgc ataatgcttc gagggactgc atatattcat cgccgctgac aaagagcgca
     9481 ttattgatcg cgataagaga accgccgtca ttgatcaacg gacggacctt attgataagc
     9541 cgcgcgcttt catgcacttg atccaccctg cctttggatg tgctggagaa aaacggcgga
     9601 tcgatcagca cataatcaaa aaaggtgttg gtacgtttca tgctgctgac ttgttcgaag
     9661 aaatctctgg cgatgaaatc tcctttacgg atcggaaacc cgttcaaagt gtaggatgat
     9721 tttgcaaggt tgagaaattc acggttcaag tcaatttgca cgacacggtt tgccccgcct
     9781 ttaagtgcgg ctacgccgaa actccctgtg taggcaaatg tgttcaagat cattttgtct
     9841 ttggcatttt cgatgagcca ttttcgcagg ttccgcgtat cgagataaaa actggcatct
     9901 cgattcaaaa tggggttaat ggcataccag gtatcgtgtt cttttatttt tcggtcgaac
     9961 tcagtgccga ataacagagt gccgcgtttt tcctcctgtg taccatggcg ggttttgaca
    10021 attcccgcac gcagccaatt caacgatgcg cgcagaaatc gagaggcttc ctcaacgagc
    10081 gcctgaccgt gttcaaaatc gtctgcgtag ttgtggatca cagcggtgga cgcatacaca
    10141 tcaatggcaa gctcggggca gccttcgagg aatccgttga aaagacgaaa ggccgattcg
    10201 tgcttggaat cgatccatgc agagcgggac gcgagtattt tttccagcag gggggtgagc
    10261 ggtatgtttg tcatttgggg tggtgagatt atatcccttc cacattcaac actggcaatg
    10321 tgatattcaa caccttgaca ttgccattct acgggagtaa aaacaaaccg accagtcggt
    10381 ttttatcacc tccccatttt agaagcagga aatcatgcaa cagagaagcg aagaaacccg
    10441 ttcaaaaatt cttggggctg ccatcaagtt attttccacg cgtgggttta actcggcaag
    10501 cgtggacgat atctgcaggg atgcgggcat cagcaaaggt gcgttctacc atcatttcaa
    10561 aagcaagcag gcccttttcc tcgccttgct tgatggctgg ctgcaaacca ttgacaatgc
    10621 cattgaagca tcgaaggaca agaccgtgcc tgagaccttc atgcagatca ccgaggcctt
    10681 tccctatatc tttgaaaccg caggtgaagg cctgcccatg ttccttgaat tttggatgca
    10741 agccagccgc gatgaaaaga tctggcaggc gagcattgct ccctaccgcc gttatcaaaa
    10801 gcatttcacc cagttgatca aaaaaggcgt ggatgaaggc tcgttcgttg aagtggaccc
    10861 tgagttgacc tcgcgcatga tcatatccac ggccatgggg ttgttgctgc aaagcctgct
    10921 ggacccgaaa ggcgcaaaat gggagaaggt ggcccgcgac agcaccaata tgcttgtaaa
    10981 tagtttactg aaaaaatagg agtggcaaaa gtgattctgg gcagacttgt tgccagtccg
    11041 cgaatttagc cactccccaa aaataaaacc gtcataaccc aaagaggagc aaaccatgaa
    11101 aatcgtaaca gattgcgcgg cagatatgcc gaatgaagaa cttgaaaggc tgggcgttgt
    11161 acaagcaccc cttttcatcc aatttcctga aggcgaagtg aacgccatgg aaatttcagc
    11221 agatgagttt tacaaccgcc ttgaagccat gcgtccgcag atcccaacca cggcacagcc
    11281 atccagcgga atattcgccg agttgtatcg caaactggca cagacggaga aaaacatttt
    11341 ttctgtgcat atttcttcgg ggctgagcgg cacgatcaac tcggcacggg atggcggtga
    11401 gcaggtcaag gcggaggcga atgtcagctt ttgggatacg ctcacccttt caggcgggga
    11461 acgttttcag gtggtggccg ctgccttggc ccataaagca ggctgggcaa tggataaaat
    11521 tcaggaacgc ctcatgaaga tccgcgaaaa gaccgaggtg atctacacgc tcgacacttt
    11581 ggaatatctc gcgcgtggag gccgtattgg acgtgtgaag gcattggcag gagcgttgct
    11641 caacctcaag cccgtcatcc gagtggatac tgacggcaag tacagcaccg tcaaaaccga
    11701 gcggacgatc aacaaatcca ttgcgacgat tgcagatcat ttggtggaaa aatatggcaa
    11761 cacacccgta tgggtcactg tgctgcatgg acgttttgca gaaaaagccg acctgctggc
    11821 agatgaattc aggcagaaat taaacgtcgc caaaatggaa gttcagcgca tctcccctgt
    11881 tttgggcgta cacacaggcc caggcattgt cggcgctgcc gtagtgccaa tggaattgat
    11941 gaacgatctt ggagaataaa aaagaaagac gaaagcggaa agaaaacggg cttgcaattt
    12001 ctgcaagccc gttcaatttt atgcggcaaa tctttttgct ttttcgatcc gctcgcccag
    12061 cggcgggtgg gaatagaaca tgaagaccac ccatttttcg gggtcgatct ccccaaggtt
    12121 ttgattcgcc agccttgtga aagcggaggc aaacgcctct cccttttggg tggaactcaa
    12181 ggcgtagtca tcggccatgt tctcgcgcca acgtgaaatg gcattgttga cgggtgaagt
    12241 cagcaagcca taggcggtga ggatgagcat gagcgcgggc agggcggcga tgtctgcggg
    12301 ggaggtgaag gagaaaaagc ttacagcgga atccaatgcc agtgatgcaa tgaacaggct
    12361 gagggtagta ctgaccgtgc cgaaggcgat caaaaaagga atatccttgt ggacgtgatg
    12421 tcccaactca tgggcgagga ctgtttcaat ttcatccaaa gtgaactcgg tgatgagggt
    12481 atcgccaagg ataatgcggc gtgtgttgcc aagcccggtc aacgcggcgt tggcagcctt
    12541 tgtgcgtttg gacatgtcga acttgaagac accttttact tttgtgtttg cgcgttctgc
    12601 cagagccagc aggcgttctt ccaattcctt gtgttcctca ccaagcggga catatttatt
    12661 gaagagcggc atgatgagca cgggcgcaag attcgaaaca aggatgttga aaagcagcaa
    12721 gccgcctgcc acccacaacc accacgcatc accagtgaga cgcagggcga gatacatcaa
    12781 ctcaagcatg atcaacccaa gcggcgcgcc aatggcgagg cctttgacct gatccacgag
    12841 ccaatctttg agtgactgat tcgattgtcc aaaccgatgc ggcaggacaa atccgctgta
    12901 ataactgagg ggtagattga tgatggcaga gatgccgccg aagacggcca cgtacaacgc
    12961 gatgataagc caatcggcgc ctgtggtcac cgaccattgg acagtgacca gttgatgaag
    13021 ccattcgcgg agtgaaatgc tccaaccaag gaaaagccat gccagagcat aggccacgcc
    13081 aaaaagcgta tccaccagcc aaaggcggcg attaattcgg gaatattctt ttgcctgttt
    13141 ttgtttttcg aggtcgaggg cggtcatggg gagtgtgagg ttgaa
//
LOCUS       DAOQLC010000119        13167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1769_length_13167_cov_25.432657, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000119 DAOQLC010000000
VERSION     DAOQLC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1769_length_13167_cov_25.432657"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1944)
                     /locus_tag="PL000_17065"
     CDS             complement(16..1944)
                     /locus_tag="PL000_17065"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HNC90642.1"
                     /translation="MISRIRVGIKYLFQLGFRPLALYGLYKFGLKTGHYRRVTPASAD
                     TIIPNSHSLFVLPDRDLLAQILGEDGKTDLLNAADEIAAGKVRVFGELTPLDFQLNQP
                     LRHWTEYESKPALLSSFFFLHNDIKFLWEPARFGWAFILGRAYHLTQDEKYADAFWRH
                     FEAFTDANPVNMGPHWMNGQEVAIRMMALVWCAHVFAKAPIANYQSLVTAIYQHAARI
                     PSTLVYARAQNNNHLITEAAALYTAGLFFKNKTLRESGWHWLNWCFQNQIGDFGEYIQ
                     HSANYHRLMLQTALWVNLIKQDVFSAHTSQAIARSAHWLFSLLDNVSGLTPNLGANDG
                     ALIFPLSAADFRDYRPTVQAASRAFLRTSLPSGMWDEMSLWFGLPANERVINSDAYLT
                     ENVRGKNSWAYLRASRFRSRLSHMDQLHLDLWWRGLNIAQDAGTYLYNAESPWDNPLV
                     TTRVHNTVTVNGRDQMTRAGRFLVLDWINAYSKNLIEADDEILGRTRAYHRAYHGLKH
                     ERTVTVYQNERWVVNDKFSARSRSERTYRLHWLLPDYEWELENQDDAHGIRLRIKSPS
                     GWITLAVHSSFPLQLSSFSLVRAGELVYGQREVQPYEGWVSHIYGQRAPALSFVVEMT
                     SAYDTELTSEFIFPDSAA"
     gene            2133..2375
                     /locus_tag="PL000_17070"
     CDS             2133..2375
                     /locus_tag="PL000_17070"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC90643.1"
                     /translation="MEELISKLAAFIATDILKQPKRAIRADEALISSGLIDSFSLMDI
                     SLFVEGNFGVRIDDTELNAETFDTLEQLAKLIQSRQ"
     gene            2379..3830
                     /locus_tag="PL000_17075"
     CDS             2379..3830
                     /locus_tag="PL000_17075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HNC90644.1"
                     /translation="MDLSSPLFLFLFLPVFLLIYLVSAGKARTGILLLASVIFLAWGD
                     ASAILWLGGILVSGYWMGLAIAKAQETGQSAAKWLWAGLGVNVILLAFFKYLTAFGQE
                     GLLWLNTPETWVDDLSGLTVPIGLSYVTFQLIAYLVDVWKGNTPAEKNFVSFSAYLLF
                     FPKLVSGPITRYKPFAVQMEQLNPSMDDVAAGFRRLLSGFIKRTVIANQLALMANAVF
                     NQPTANVEPKFAWLGLAAYTLQIFFDFSGYTDMALGLGLMIGIRLPENFNFPYIAQSV
                     SDFWRRWHITLSTWFREYVFYPLERKRFKWMGQQVNILIVFLLTGLWHGFNPTFMVWG
                     MIHGIALAVESLGFGRWLKGAWRPLQHLYTLTIVVIGWVFFRSQDLNFAFEFFRRLAG
                     DRSNLMPLPFSQTTPMPFIEPSFIMALIAGTLFSFPLTSLWRQLRERFESRQSNSYLL
                     FQVMEDAALLLFFILGLGLQLSGSFLPNLYAKF"
     gene            3844..4911
                     /locus_tag="PL000_17080"
     CDS             3844..4911
                     /locus_tag="PL000_17080"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90645.1"
                     /translation="MSTESIARQRYSLAFVLFLLGGLLFIMISSSLAGISDFENNLIM
                     KNLLIEKTNWLHLKIGDRVFPEVVIGKDGWMEYSGDGNLDDFQTTQPLWNKHSLAKRL
                     TAFEAYLNEQGITLLIVVAPNKASIYPERLPAEIQPLTEQTRLDDFIAALKENNLPQV
                     LDLRPALQQAKLEQEVYYYSNTHWNGYGAFVAYTTILNELAKTHPELHPYTPEELDLG
                     TKRATVQDIAKLLQANFIKEPHFFYIPQGHPVQSLTLKDYYGFNHITSVDDESLPTLL
                     MIHDSFGYRFLNEYMSMNFEKAYFIHSGKDTPTYLNRAAIEQFNPDIIVIEIVERSLN
                     NLNNYLSQFDSPIKFYQEPKK"
     gene            4908..6590
                     /locus_tag="PL000_17085"
     CDS             4908..6590
                     /locus_tag="PL000_17085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC90646.1"
                     /translation="MTTLADLIHQNYSKNPDKTAIILQHAGQDDKPVSYLDLVKGSAQ
                     YAQTYAEAGIQPGEVVILILQHGEDLVYSFWGAILHGAIPAIMPFLTEKLSPERYRAD
                     LTALLSVTQPAAIVTYPEFEADIRAALTADGSVRRVLLTSQLAPRSEPDFNQLRGLQC
                     KPTDIVLLQHSSGTTGLQKGVALSHQAVLNQLNAYTRAIQLTSDDVLVSWLPLYHDMG
                     LIAGFLMPILMGITLVLQSPFDWVRAPHKLFQSVSKYKGTLSWLPNFSYNFCAQKVRE
                     RDLEGVDLSSWRVITNCSEPVKWESHQSFFQRFEKYGLRESALNCSYAMAENVFGVTQ
                     TPLGTKPVTDEIDRASYINERIANPPQDGKPALKMMSSGRALPNTRLLILSEEGKPLP
                     DKVIGEIALQSDCMLTGYYHRPDATEKALRDGWYLTGDYGYTLNGEIFVAGRKKDMII
                     VGGKNVYPQDLESLAYEVPGIHAGRAVAFGIEDTKAGTEEVVIVAEVDTDDPEERQRI
                     ADALRKHITQNSAIALRHAHLVDPKWIVKTSSGKTARSANREKFLKEMDMQS"
     gene            6633..6899
                     /locus_tag="PL000_17090"
     CDS             6633..6899
                     /locus_tag="PL000_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90647.1"
                     /translation="MSVLIAVLTIFVLVINYNLYQIWFNPKDYIDKLTYNSLPFLMLN
                     NPRLYKWYTSKTFLWFARILNLFFALLMTTFLFLVVLGSLGFFP"
     gene            complement(6982..7572)
                     /locus_tag="PL000_17095"
     CDS             complement(6982..7572)
                     /locus_tag="PL000_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90648.1"
                     /translation="MSFDEPNFDDAGDAPLPEESNNNRTFLIAVGILGGIILISIACL
                     GAVYVFGSQGRAAVQETEVAGAAMASATANAVNETVNRAITATFEASILPTATTTFTV
                     TPTSPVGVASATPTPDPALAGLSGTSVAQTATIGAAYTQAALAALSTVPPTSTALPNT
                     GFADEFGLPGIVVMSLAFVIVILLARRLRSAPETIR"
     gene            complement(7633..8688)
                     /locus_tag="PL000_17100"
     CDS             complement(7633..8688)
                     /locus_tag="PL000_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90649.1"
                     /translation="MQAPVHHIVGLTSIVRERLLPVNGTVLARLNQKVSPSDVIAEAK
                     WSREHVLLNVAHQLRVKPEAADRLIKFNFKDVVPADAEVAVGEGLFPRVVRAPREGRV
                     AAVGGGQVLLEVGESKIELRAGIAGTVIEIVPNRGVIIQTAGALVQGVWGNGRIDSGL
                     LVNLADKPESVLTADRLDVSLRGSIILAGMVKDAETLQAAAELPVRGLILSSLYPSFI
                     QLAREMRYPIVVTDGFGPMPMNSAAYRLLSTNAKRDVTVNAEVYDRYSGARPEVIINL
                     PISSDPPAPREVETFSPGLQVRMRRPPAMGAIGVISAIKSGLTKLPSGLHAHAAEVKL
                     ENGETVIVPLVNLEVVG"
     gene            complement(8693..10450)
                     /locus_tag="PL000_17105"
     CDS             complement(8693..10450)
                     /locus_tag="PL000_17105"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNC90650.1"
                     /translation="MASSLIDGNSILAIDVGAANTRAVFFDVVEGEYRFVASGVAPST
                     AEAPYKDVSEGVRNAIQNLQSVLGRSLLDGSRNLITPSQPNGSGVDALVATLSAGPTI
                     KTVLVGLLKDVSLESARRLAESTYMRIVETLNISDRRKPDQQIDAVLRARPDLIVIAG
                     GTDGGASRSIQKLLEPIGLASFTMPEEKRPAVLYAGNEKIEAEIKTLIGSLATSLHFS
                     PNVRPSLDTEDLEPAQRELARMTINIRKRQIRGVDLLDMWSGGHLLPTAYATGRMVRF
                     LSKVYSSQKGILSVDLGASAAIIAAGFSDKSSLNVYPQFGLGENLTGLLNYTKLEDIL
                     RWSSLDVSPGVLRDYMYQKALYPSTIAATKEDLDMSQAIARQALFLAMQSARRDFPRN
                     VAGIKPNLTPLFEPILAGGGILSDASRPGQSLLLLLDGIQPVGVTTVIIDQNNLLPLL
                     GVTASQNNILPVQVLESGAFLSVGTVVAPVTSAAYGTPILRAILTYENGTEARMDLKY
                     GGLETLPLANGQTGKLTVQCLRGTDVGFGPGRGGTIPVSGGALGVVFDGRGRPLDLPA
                     DSVRRRDLIKKWNWTLGGE"
     gene            complement(10453..11166)
                     /locus_tag="PL000_17110"
     CDS             complement(10453..11166)
                     /locus_tag="PL000_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90651.1"
                     /translation="MVLTPQMLDMITGALSLLFTILIFSYVLGDNPLFRVAVYIFVGI
                     SAGYIASVAIWQVIWPKLLYPLVVGPSTPFESIGYLVFPLLGFALILMKISPRLAGLA
                     RFVMAFIVGVGAAVAIAGALSGTLIPQVSGAINSFDMVAAGARNVGFIEALVNGGTIL
                     AGTIFTLIYFHFGARPKADGTMHRFALIEIPAWIGRLFIGITLGVVFAGVYSAALTAL
                     IERIDSIINFINSFIGNFQ"
     gene            complement(11168..>13167)
                     /locus_tag="PL000_17115"
     CDS             complement(11168..>13167)
                     /locus_tag="PL000_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90652.1"
                     /translation="PDWLRSMSAESGAQNSAPVSSSNDLFGASSTDDTPDWLRSMSAE
                     SSPQDSAPVSSSNDLFGSATTDDTPDWLRGMQAQSAEPAASSEPVSSGFPESSADLFG
                     GNVPDWLKGTAEEKSTAAPLSEPDWLKSESENTPVDNAPDWASAGVTSGDELEMPAWL
                     NPAESAAQPSEAQDISLGDVPDWLKASAPKETIFGETPAADPAAVPDWLNSLQSAAPE
                     SSSKPAEIEFSSTPPAEAGIDNLFTDLPDWLSKADDMAAPVSSSPSPITNTDAIAPGD
                     LPSWVQAMRPVDSGLPTSMPVSLSSDQTLESRGALAGLQGVLPAVPGFSPASKPKAYS
                     IRLQANEEQQSHAALLEQILAAETAPVPLSSLSNLNTSRALRWFLVAVFFLILPAILI
                     MRTQFFALPPSELKPAMKGALDVAQLLPEGAPVLVAFDVEPARVGEMEAVAAPMFDQM
                     LLLHHPRFTFVSTSESGAILAERFISTGPLAGHQYQSGIEYMNLGYLPGGQMGIYSFA
                     QNPRQSAPYLFNSGVTESPLLNAYLSPAWAAEPWSGITTLSQFTAFVIVTDNADSARA
                     WIEQTSSTRGAMPIVVISSAQAAPMIRPYFDSQQISGLVAGLYDGAVFEQNNLNRPGT
                     TRIYWDAYNIGLLLAMVLILLGGLWNLVLGLRDRAAAREVK"
BASE COUNT         3323 a         3587 c         3330 g         2927 t
ORIGIN      
        1 cttgaaaata ttttatcacg ccgctgaatc agggaagatg aactcgcttg ttaattcagt
       61 gtcataggcg gaagtcatct caaccacaaa cgacagcgcg ggagccctct gcccatatat
      121 atgagacacc cagccttcat acggttgaac ctctcgttga ccgtaaacca attcaccggc
      181 gcggacgagg gaaaatgacg aaagctgaag ggggaaggat gaatgaactg cgagtgtgat
      241 ccacccagat ggagatttga tacgaagcct gattccgtgc gcatcgtcct gattctccaa
      301 ctcccattcg taatcgggga gcagccagtg taagcggtag gttcgttctg agcggctacg
      361 cgcagaaaat ttgtcattta ccacccacct ctcgttttga taaaccgtca ccgtgcgctc
      421 atgtttcaaa ccatgataag cccgatgata ggcccgtgtg cgacctaaaa tctcatcatc
      481 cgcttcgatc aaattctttg agtaggcatt gatccaatcc aacacgagga agcgacctgc
      541 acgagtcatt tggtcgcggc catttacggt gacggtgttg tgaacacgcg tcgtcaccag
      601 cggattatcc cagggcgatt cggcgttgta caaatatgtc cctgcatcct gggcgatgtt
      661 taatccgcgc caccacaggt cgagatgcaa ttggtccatg tgcgaaaggc gcgaacgaaa
      721 acgcgaagcc cgcaggtacg cccatgaatt ttttccacgc acgttttctg ttagataggc
      781 atcggagttg atcactcgtt cgttggcggg caggccaaac cacaaggaca tttcgtccca
      841 catgccagag ggcaggctgg ttcgcaaaaa agcacgcgat gcggcttgca cggtcgggcg
      901 gtaatcgcgg aagtcggcgg cgctcaaagg gaagatcaat gccccatcgt tggcacccag
      961 gttcggggtc agccctgaaa cgttatcgag gagagagaaa agccaatgag cagaacgtgc
     1021 aatcgcctgc gatgtgtgtg cggagaagac atcctgtttg atcaaattga cccacagggc
     1081 cgtttgcagc atcagccgat gataattggc gctgtgctga atgtattcgc caaaatcgcc
     1141 gatctggttt tgaaaacacc agttgagcca atgccagcca gattcacgca aggtcttatt
     1201 tttgaagaac aaccccgctg tgtacagcgc agcggcctcg gtgatgagat gattgttatt
     1261 ttgtgcgcgg gcatacacga gtgtggaggg aatgcgcgct gcatgctgat aaatggcggt
     1321 gacaagagac tggtaattgg caattggtgc tttggcaaag acatgcgcac accagacgag
     1381 agccatcatt cgaatcgcaa cttcctgtcc gttcatccaa tgcggcccca tgttgacggg
     1441 attcgcgtcg gtgaaggctt cgaaatgcct ccaaaatgca tcggcgtatt tttcatcttg
     1501 cgtgaggtga taggcgcggc caaggataaa cgcccagccg aagcgggcgg gctcccagag
     1561 aaatttgatg tcgttgtgaa gaaagaagaa tgaagaaagc agcgctggct tcgattcgta
     1621 ttccgtccaa tggcggagag gttggttgag ttggaaatcg agcggggtta attcgccaaa
     1681 cacacgaact tttcctgcgg cgatctcatc ggctgcgttg agaagatctg tttttccatc
     1741 ttcgccaagg atctgcgcca gcagatcacg atcggggaga acaaacaggg aatgggaatt
     1801 gggaattatg gtgtcagcgc ttgcaggtgt gacgcgcctg taatgccctg ttttcaaccc
     1861 aaatttatac aagccataca aagccagcgg gcgaaagccc agctgaaaaa ggtatttgat
     1921 cccaacacga attctcgata tcatggttcc caaagtttac cagccaattt gcaaaagggc
     1981 ggcattcccc cgccactgat agtgtagaat tcggggctga ccgcagcaca catcaggatt
     2041 gatccctgct gccttcaacg cgaacaagcc aagaaaattc gtgaaaaact tgtacgataa
     2101 agaaattaca acccaaaaag gaaccattca tcatggaaga actcatttca aaacttgccg
     2161 ccttcattgc aaccgatatt ctcaaacaac ccaaacgcgc cattcgcgcc gatgaagccc
     2221 tgatctccag tggattgatc gactccttca gcctgatgga tatcagcctg ttcgtggagg
     2281 gaaatttcgg cgtccgtatc gatgatacgg aactgaatgc agaaaccttc gataccctgg
     2341 aacaactcgc caagctgatc caatcacgcc agtaattcat ggacctttct tcgccgctct
     2401 ttctcttcct gtttttgcca gtctttctgt tgatctatct ggtcagcgct ggcaaagcgc
     2461 ggacgggcat cctcctgctt gccagtgtca tctttctggc atggggagat gcttctgcca
     2521 ttttatggct gggcggcatt ctggtcagcg gatattggat gggactcgcc attgcaaaag
     2581 ctcaagagac gggtcaaagc gcggcgaaat ggttgtgggc agggttgggg gtcaatgtaa
     2641 ttctgctcgc cttcttcaaa tatctaaccg cctttgggca ggaaggtttg ctctggctca
     2701 acacccctga aacatgggtg gatgacttga gtgggttaac cgtacccatc ggcctttcct
     2761 atgtcacgtt tcaattgatc gcctatctgg tggatgtgtg gaagggcaat acgcccgccg
     2821 aaaaaaattt tgtttccttt tccgcctatt tgctgttctt tccaaaactt gtctctggcc
     2881 ccatcacccg ctacaaaccg tttgcagtgc aaatggagca actcaaccca tccatggacg
     2941 atgtggctgc agggtttcgg cgcctgcttt cagggtttat aaaacgtact gtgatcgcaa
     3001 atcagctggc gctgatggcc aacgccgttt tcaaccagcc gaccgccaat gtggaaccga
     3061 aattcgcctg gctgggattg gccgcataca ccctgcagat cttttttgat ttttcaggct
     3121 ataccgatat ggccctcggg ctgggattaa tgatcgggat ccgcctgcct gaaaacttca
     3181 attttcccta catcgcgcaa agtgtcagcg atttttggcg gcgctggcac atcaccctct
     3241 ccacctggtt tcgtgaatat gtcttctatc cgttggagcg caaacgtttc aaatggatgg
     3301 gacaacaggt caatatcctc atcgtcttcc tgttaactgg tctctggcat ggcttcaacc
     3361 ccaccttcat ggtttggggc atgatccacg gcattgcatt ggcggtggaa agcctcggct
     3421 ttgggcgctg gctcaaaggg gcatggcggc cattgcagca tctctacacc ctgaccatcg
     3481 ttgtgatcgg ctgggttttc ttccgctcac aagatctgaa ttttgccttc gaattcttcc
     3541 gccgcctggc aggtgacagg tccaatctta tgccgctgcc attcagccag accacaccca
     3601 tgccattcat tgaaccgtct ttcatcatgg cattgatcgc agggacactg ttctcgttcc
     3661 cactcacctc gttgtggcgg caattacgcg aacgattcga atcacgccag agcaattcct
     3721 acctcctgtt tcaggtcatg gaagatgcgg cactgctcct gtttttcatc cttgggctgg
     3781 ggcttcaatt gagcggcagt ttcctgccaa acctgtatgc aaaattctaa aatggaaaaa
     3841 gtcatgtcca ccgaatcgat tgcacgccag cgatattccc ttgccttcgt tttgtttttg
     3901 ctgggcggcc tgctttttat catgatctca agcagtctgg ctggaatttc agattttgaa
     3961 aataatctca tcatgaaaaa cctgctcatc gaaaaaacca actggctgca cctcaagatc
     4021 ggagacaggg ttttccctga agtggtgatc ggaaaagacg gctggatgga atacagcggc
     4081 gacggcaacc tcgatgattt ccaaaccaca cagccgcttt ggaacaaaca ttcgctggca
     4141 aaacggctga cggctttcga agcctactta aacgagcaag gcatcaccct gctgatcgtg
     4201 gttgccccca acaaggccag catctacccc gaacgcctcc ccgctgagat tcagccgctt
     4261 accgagcaaa ccaggctcga tgactttatc gccgcgctaa aagaaaataa cctgccccaa
     4321 gtattggacc tccgccctgc cttgcagcag gcgaaactcg aacaggaagt ctattactat
     4381 tccaacaccc actggaacgg ctatggcgca ttcgtggcat ataccaccat ccttaatgaa
     4441 ctcgccaaaa cccaccccga actccatccc tacacaccag aggaactgga tctgggcaca
     4501 aagcgcgcca cggtacagga tatcgccaaa ttgttgcagg ccaatttcat caaagaaccg
     4561 catttcttct acattcccca aggccaccct gttcaatcgc tcacgctgaa agattattac
     4621 ggcttcaatc acatcacctc tgttgacgat gaaagcctgc ccaccctgct catgatccac
     4681 gattcgttcg gctaccgctt tctcaacgaa tacatgagta tgaatttcga aaaggcatat
     4741 ttcatccaca gcggaaaaga cacccccacc tacctcaacc gtgccgccat tgaacaattc
     4801 aacccggata taatcgtcat cgagatcgtg gagcgaagtt tgaacaacct caacaactat
     4861 ctttcccaat tcgattcccc catcaagttt tatcaagaac ccaaaaaatg accacgcttg
     4921 ccgacctgat tcatcaaaac tacagcaaga atccagataa gaccgccatc atcctccaac
     4981 atgcagggca ggatgacaaa cccgtttcct atcttgacct ggtaaagggt tccgcacaat
     5041 atgcccaaac ctacgccgaa gcagggattc aacccggcga agtggtcatt ctcattttgc
     5101 agcacggcga agacctggtc tattcatttt ggggtgccat cctgcatggt gcaattcccg
     5161 ccatcatgcc gttcctcacc gaaaagctct cccccgaacg gtatcgcgcc gatctcaccg
     5221 cgcttctttc ggtcacgcag ccagccgcca ttgtcaccta ccccgaattc gaagcagata
     5281 ttcgcgccgc actgacagcc gatggctccg tccgcagggt tttgctcacc agccaactcg
     5341 cgcccagatc cgaaccagat ttcaaccaac tgcgcggctt gcaatgcaaa cccaccgata
     5401 tcgtcctgct ccagcattct tcaggaacca caggtctgca aaaaggcgtc gccctttcac
     5461 atcaggcggt gctcaatcaa ctcaacgcct acacccgcgc catccaactc accagtgatg
     5521 atgtgcttgt ttcatggctg cccctgtatc acgacatggg tctcattgca ggcttcctca
     5581 tgcccatcct catgggcatc accctcgttc tacaatcccc gttcgattgg gtgcgtgcgc
     5641 cgcacaagtt atttcaatcg gtcagcaaat acaaaggcac actttcgtgg ctgccgaatt
     5701 tctcctacaa cttctgcgca caaaaggtcc gcgagcgcga tcttgaaggc gtggaccttt
     5761 catcgtggcg tgtcatcacc aattgctccg aacccgtcaa atgggagagt catcagtcct
     5821 tttttcaacg cttcgaaaag tacggcttgc gcgagtccgc gctcaactgc agttacgcca
     5881 tggcagagaa tgttttcggg gtcacccaaa ccccgctcgg cacaaaacca gttaccgatg
     5941 agattgaccg cgcgtcctac atcaacgagc ggatcgcaaa tccgccccag gatggaaaac
     6001 ccgcgttgaa aatgatgtca tcgggacgcg cgcttcccaa tacccgtttg ctgattctgt
     6061 ccgaagaggg aaagcccctc cccgataaag tgatcggtga gatcgccctg caaagcgact
     6121 gcatgttgac gggttattat caccgccccg atgccaccga aaaagcattg cgcgatggct
     6181 ggtatctcac aggagattac ggctacaccc tgaacggcga gatctttgtg gcaggccgta
     6241 aaaaggacat gatcatcgtc ggcggcaaga acgtgtatcc gcaagacctg gaatcgctgg
     6301 cttacgaagt gcctggtatt catgcaggcc gcgccgtggc atttggcatc gaagatacaa
     6361 aagcagggac agaagaagtg gtcatcgtgg ccgaagtgga taccgacgac cccgaagaac
     6421 gccagcgcat cgccgatgcc ctgcgaaaac acatcaccca aaattcagcc atcgccctgc
     6481 gccacgccca cctcgttgac ccgaagtgga tcgtcaaaac ctccagcggc aagaccgccc
     6541 gctccgccaa ccgcgaaaaa ttcttaaagg aaatggatat gcaatcatag ttttaagtat
     6601 tgccaaagcc aactgacaaa atgagcaaac ccttgtccgt tcttattgcc gtgctcacta
     6661 ttttcgtcct tgtgatcaat tacaacctgt atcagatctg gttcaacccc aaagactaca
     6721 tcgacaagct gacatacaac tcattgccgt ttctgatgtt gaacaatccc cgcctttaca
     6781 aatggtacac ctcaaaaacc tttctatggt ttgccaggat tttgaatttg ttttttgctt
     6841 tactaatgac cacgttttta tttctggtcg ttttgggaag cctgggcttt tttccctgat
     6901 aaatgtgctt cacgataagc ttttaaacga aaacgggatg gccgcataag cgtgccatcc
     6961 cgttcatatt tatgggaatg attatcgaat tgtctcgggc gcgcttcgta atctgcgggc
     7021 cagcaaaatc acaatgacaa aggccagtga catcaccacg atgccaggca aaccaaattc
     7081 atcggcaaaa cctgtattcg gcagcgctgt ggacgtgggc ggcacggtgc tcaacgccgc
     7141 aagggcagcc tgtgtgtatg ccgcgccaat ggttgcggtc tgtgcaacag aggtgccgct
     7201 caacccagca agagcggggt caggtgtggg ggtggcagat gcaacaccaa cgggagaggt
     7261 cggagtgact gtaaaagtgg tcgtggctgt cggcaaaatg gacgcctcga aagtggcggt
     7321 gattgcgcga tttaccgtct cattgaccgc attggcagtg gcggaagcca tggctgcacc
     7381 ggcaacttct gtctcctgta cggcagcgcg gccctgtgaa ccgaaaacgt aaaccgctcc
     7441 gaggcaggca atggagatca ggataatgcc gcctaaaata cccaccgcga tcaaaaacgt
     7501 tctattgtta tttgattctt cgggaagcgg ggcatcgcca gcatcgtcga aattgggttc
     7561 atcaaaagac atggaacact ctcctcgtga tatcaccttc aacaggtgat ttaattacaa
     7621 ctattttgaa tattatccca caacttcaag atttacgaga ggtacgataa cggtttcgcc
     7681 attttcaagc tttacttctg cagcatgggc atgcagaccg ctcggtaatt tggtcaaccc
     7741 ggatttaatg gccgagataa caccgatcgc ccccattgca ggcggacggc gcatgcgtac
     7801 ctgcaaacct ggcgaaaaag tttcaacttc acgaggggcg ggcgggtcgc tggagatggg
     7861 caggttaatg atgacttccg gacgagcacc gctgtaacgg tcatacactt cagcattgac
     7921 ggtgacatca cgtttggcat tggtactgag caggcgatac gctgccgagt tcatcggcat
     7981 ggggccaaaa ccatcggtca caacgatcgg gtagcgcatt tcacgagcca attgaataaa
     8041 ggacgggtac aaactggaaa ggatcaaccc acgaacaggc aattcggcag cggcttgcag
     8101 ggtttcagca tccttgacca taccggcaag aatgatggac ccgcgcaggc ttacatccag
     8161 acgatcagcg gtcaacacgc tttcgggttt gtctgcaagg ttgacgagca accccgaatc
     8221 aatgcgtcca ttgccccaaa cgccttgtac aagcgcgcct gcggtttgaa taatcacacc
     8281 acggtttgga acgatctcga tcacggtacc ggcgatgcct gcacgtaatt cgatcttgga
     8341 ttcacccact tccagcaaaa cctgtccgcc gccgacagct gccacacgac cttcacgcgg
     8401 ggcgcggacg acccgtggga acaacccctc cccaactgcc acctcggcat ccgcagggac
     8461 aacatccttg aaattgaatt tgatcaggcg gtctgccgct tcaggcttta ctcgcaattg
     8521 gtgagcaaca tttaataaca catgttcgcg tgaccatttc gcttcggcga tgacatcgga
     8581 cgggctgacc ttttgattca ggcgtgcaag cacagtgccg ttcacgggca aaagccgttc
     8641 gcgcacaatg gatgtcaggc caacgatatg gtgtacgggc gcttgcatga gtctattcgc
     8701 ctccgagagt ccaattccat tttttgatca aatcacgacg acgcacacta tcagcaggca
     8761 aatcgagtgg acgtccacgg ccatcgaaga ccacgcccaa tgcgccgcca ctgaccggga
     8821 tcgtgccgcc gcgtccgggg ccaaaaccca catcggtgcc gcgcaggcat tgaaccgtta
     8881 acttaccagt ctggccattt gccaacggca gggtttcaag accgccatat ttcaaatcca
     8941 tgcgggcttc ggtgccgttt tcataggtca gtatggctcg cagaattggg gtgccataag
     9001 ctgcggatgt cactggggcg accaccgtcc caacgctgag gaatgcaccc gattcaagca
     9061 cttgaacagg gagaatattg ttctgagatg cggtcacacc caacaggggc agcaggttat
     9121 tttggtctat gatgacggtg gtcacaccca caggttggat cccgtcaagg agcagaagca
     9181 ggctctggcc ggggcgcgaa gcgtcgctca aaattccacc gcccgccaga atcggctcaa
     9241 agagcggagt cagattcggc ttgataccag ccacattccg tggaaaatca cggcgagccg
     9301 attgcattgc aaggaaaaga gcctgccgtg caatggcctg cgacatatca aggtcttcct
     9361 tggtggcggc aatggtggat ggatataaag ccttttgata catgtaatcg cgcaacacgc
     9421 ccggcgagac atcgagcgaa gaccagcgca aaatgtcttc gagttttgtg taattaagca
     9481 aacctgtgag gttctcgcca aggccgaatt gtggatacac attcaatgaa gatttgtcac
     9541 tgaaacctgc tgcaatgatc gcagccgagg cgccaaggtc cacgctcaaa attccctttt
     9601 gagaactgta caccttggaa aggaagcgaa ccatgcgacc agtggcatag gcggtcggca
     9661 gcaggtggcc gcccgaccac atatccaaaa ggtccacgcc tctgatctgg cgtttgcgga
     9721 tgttgatggt catgcgggcc agttcacgtt gggcgggttc gaggtcttcg gtatcaagag
     9781 aaggacgcac attggggctg aagtgcaggg aggttgccag tgaaccgatc agggttttga
     9841 tctctgcttc gatcttttca ttgcccgcgt ataacacagc agggcgtttc tcttcgggca
     9901 tggtgaaact cgccaggccg atcggctcca gcaatttttg aatggaacgg gatgcacctc
     9961 catcggttcc acctgcgatc acgatcaggt cgggtcttgc ccgcagcacc gcatcgatct
    10021 gttgatcggg tttacggcgg tcactgatat tcagggtttc aacgatccgc atgtaagtgg
    10081 attccgcaag acgacgcgcg ctttcaagcg atacatcctt gagcaagcca acgaggactg
    10141 ttttgatggt gggtccagcc gacaaggttg caaccaacgc atccacaccc gacccgttgg
    10201 gctggctcgg tgtaatcagg ttacgtgaac catccagcag ggagcgtcct aaaaccgatt
    10261 gcagattttg aatggcattg cggacacctt cgctcacatc tttgtacggc gcttcagcag
    10321 tagagggcgc aacgccagaa gccacaaaac ggtattcccc ttcgacaaca tcgaagaaga
    10381 ccgctcgtgt attggccgcg ccgacatcaa tggcgagtat ggaattaccg tcgatcaggg
    10441 aggaagccat aattattgga agttcccaat gaaactgtta ataaaattga tgatcgagtc
    10501 aatgcgttcg atcagggcgg ttaaggctgc cgagtacaca cccgcaaaga ccacacccag
    10561 ggtgatgcca atgaacaagc ggccgatcca tgcagggatt tcgatcaagg cgaagcggtg
    10621 catggtgccg tccgctttgg ggcgtgctcc aaagtgaaag tagatcaggg tgaagatcgt
    10681 acccgccagg atggttcccc catttaccaa tgcctcgata aacccgacgt tacgtgctcc
    10741 tgctgcaacc atatcgaagg aattgatcgc accagaaacc tgagggatta atgtacctga
    10801 aagagcaccc gcgatcgcca ccgcagcccc aacaccaacg atgaaagcca tcacaaagcg
    10861 agcaagcccg gccaatcgcg gcgaaatctt catcaaaatt aatgcaaacc ccagcagggg
    10921 aaagacaagg tatccgatac tttcaaaagg agtggatgga ccaacgacca aaggataaag
    10981 gagcttcggc caaatcacct gccaaatggc tacagaagca atatacccgg ctgagattcc
    11041 cacgaaaata tagaccgcca cacgaaaaag cggattgtct ccaagcacat aactaaagat
    11101 caaaattgta aataacaggc taagcgcccc ggtgatcata tccagcatct gaggtgtgag
    11161 aaccatgcta cttcacctcc ctggccgcag cgcggtcgcg cagacccaag accaaattcc
    11221 acaacccacc cagcaggatc agaaccatgg ctagcagcag gccaatgttg taggcatccc
    11281 aataaatgcg tgtggtgccc gggcggttga ggttattctg ctcgaacaca gcaccgtcat
    11341 acaaacctgc caccaacccg ctgatctgct gcgagtcgaa atacgggcgg atcatcggtg
    11401 cagcctgcgc gcttgagatc acaacaatgg gcattgcacc acgcgtagaa cttgtctgtt
    11461 cgatccatgc gcgggcagaa tcggcgttat ctgtcacaat gacaaaagcc gtgaattgtg
    11521 aaagggttgt aattccactc catggttcgg ctgcccaggc agggcttaga taggcattga
    11581 gcaagggact ttcagttaca ccgctgttga acaaataggg agcgctttgg cgcggatttt
    11641 gagcaaagga ataaataccc atttgtccac cgggcaggta acccaaattc atatattcga
    11701 ttccactttg gtattggtgc ccggccaaag gtccggtaga aatgaagcgt tccgccaaaa
    11761 tcgcacccga ttcactcgtg gaaacaaatg taaagcgcgg gtgatgtaaa agcaacatct
    11821 ggtcaaacat cggtgcagcc acggcttcca tctcacccac acgagcaggc tcgacatcaa
    11881 aggccaccaa cacaggcgca ccctctggaa gcagttgagc cacatccaaa gcgcctttca
    11941 tcgcaggctt taattcactg ggtggcaggg cgaagaattg cgtccgcata attagaatgg
    12001 cgggcaggat caggaagaac accgccacaa ggaaccagcg caatgcgcgg gatgtattca
    12061 aattggaaag cgaagaaagc ggaactggag cggtttcagc ggcgaggatc tgctccaaca
    12121 aagccgcgtg tgattgctgt tcttcgttcg cttgcaaacg gattgaataa gccttgggct
    12181 tgctcgccgg agaaaagcct ggcacagcag gcaacacacc ctgcaaacca gccaacgccc
    12241 cccgcgactc aagtgtttgg tcgctggaaa gcgaaacagg catggatgtc ggcaaacccg
    12301 agtccactgg ccgcatggct tgcacccagg aaggcaaatc accaggagcg atcgcatcag
    12361 tattggtgat cggtgatggt gaggaagata ctggtgcagc catgtcatcg gctttcgaaa
    12421 gccaatcagg aaggtcggta aagaggttgt caatccctgc ctcggcggga ggagtggagg
    12481 aaaattcaat ctcagcaggt ttcgatgaag attcaggcgc agccgattga agcgagttga
    12541 gccagtctgg tacagcagca gggtcggcag cgggtgtctc accaaagatg gtttctttcg
    12601 gagcagaagc cttcaaccaa tcaggcacat cacccaacga aatatcctgc gcttccgaag
    12661 gctgtgcagc agattccgct ggattcaacc atgcgggcat ttccagttca tcaccggaag
    12721 tcacacctgc actcgcccaa tcaggggcat tgtcaacagg tgtattttca gattcgctct
    12781 tcaaccaatc aggttcggac aaaggcgctg cggtggattt ttcttccgct gtgcctttta
    12841 accaatctgg gacatttccg ccaaaaagat cagcggacga ttcagggaat ccactggaaa
    12901 ctggttctga agaagcggca ggttctgcgc tttgagcttg cataccgcgt aaccagtccg
    12961 gtgtatcgtc ggttgttgcc gagccgaata agtcattgga actgctgact ggcgcactat
    13021 cttgcgggct gctttcagca gacattgagc gcagccaatc cggtgtgtca tcggtggaag
    13081 atgcaccgaa caaatcatta gaactgctga caggcgcact attttgcgca ccactctcag
    13141 cagacattga gcgcagccaa tccggtg
//
LOCUS       DAOQLC010000120        13057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1797_length_13057_cov_26.191509, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000120 DAOQLC010000000
VERSION     DAOQLC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1797_length_13057_cov_26.191509"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..2557
                     /locus_tag="PL000_17120"
     CDS             29..2557
                     /locus_tag="PL000_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC90653.1"
                     /translation="MQKYLPVLRARRERRLQKQNKQANNARGAILSGGIVLSLLLGVF
                     IILGAFAYADVTRELPSVEILPRLLNPPDGLLLEPTHIYDRTGTHILFTFAPVDEQRR
                     YIPANPQNPQHIPANLIDAIIAVQDPQFRKHSGYSLQGLDDANQHPTIAQKLIGGLVI
                     YNEPPSLRRAIRERILAAQITAQFGRTQVMEWYLNSAHFGNYAYGVEAASQLYFGKAV
                     EQLTLAESAILAAASESPALNPLDAPQVALQRGREVLYVLNGLGIVSDEATEIALAEE
                     PEIRASTEDDANQPDPEPAPAFVNMVLAQLDTQLPRDRVERGGINIITTLDYDLQQQA
                     ACVTEVYAVRMAGLEEPKEACDAARFLPSLPPAVTLTDSSASALILDPNHGQVLAVVG
                     ETVQGQETPLVTAHSPGSSLDAFVYLTGFTRGLSPATLAWDIPGEGIIQNFDGEYHGP
                     VRLRLALANDYQVPMEALKVQMGVENVSGIASSFGVALDKQVTMLQLAGAYGVFGKQG
                     VYFGQNMNDEFLPSAVLRVEGVDRSLLLDWSLPQAKPVLTPALAYLMTHVLSDEASRW
                     PSLGRPNVLEIGRPAGVKVGQSANGLDAWVVGYTPAHVVVTWTGVHGEAESVTPRFPA
                     ALWSALMKVASGDAPADGWTAPQGVSTITVCDPSGMLPTKECPRLVSEVFLNGSEPVQ
                     ADTLYREFGINRETGLLATVFTPPELIETRIYMLVPESARDWALSAGLEIPPASYDAI
                     QAPPVNPFANIVEPKLFAEVGGVVKIMGTAAGENFQYYRVQVGKGLNPQEWVQLGGDV
                     ISPVEDGLLAEWDTSGLSGLYAVQLLVVRADQIVDTAVIQVTIK"
     gene            complement(2550..6305)
                     /locus_tag="PL000_17125"
     CDS             complement(2550..6305)
                     /locus_tag="PL000_17125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC90654.1"
                     /translation="MSSEPNEYNALNEAPAQDTLTGAYTRATFKASLHALIEQAQRDN
                     TTCSLMVIDLDHFKSINDAFGHAKGDEVLSGAAHLILQNTRASDLFFRYGGDEFVLLL
                     PNTTKTEAFDLAQRLLGIIRAQSFSRDTVLTLTLSIGVAAFPEDASDGQSLFEKADAR
                     NYEAKRRGRACVISRDGGTTSDLSFIKPSRLIERDASLELAQNFLSALVSQQHGVFGV
                     IGPNGVGCSRFLVEVEKRARLQGFEVIALRGNPQLMARPYGVLENILQNPDGTAFTQN
                     NLEKIIKAFQRRLTESGRDRLLISVDDLPQLDWGTLDLLRQILSSNEFPVLGLVYATD
                     PDHARVTAPFRAALSEYVTLSPLTPAGLQVWLRTLLQWDPPNDFIHWLEKHTHGLPAQ
                     AEQVLKHMLKEGALTRQDSGWHLSENYATIGAAGMRQWQKRVSPNNLPAALTSFVGRE
                     TELKDVRQLLATTRLLTVVGPGGMGKTRLTLQVAKEAINGFRNGVWLVELAPVTDPAL
                     ALYTIATVLGVEQVQSHSLMETVCTWLSDKEILLILDNCEHLIDMCAQFSHEVLSTSP
                     SVRILASSREALDIAGELVYRMPALETPNIQAEVPAGQLMQYAAARLFAERAAFALST
                     FRVNAEEAPLIAQICRQLDGIPLAIELAAARLRTLTLEEIATGLNDRFNLLTRGNRAA
                     APRQQTLRALIDWSYDSLSENERKLFCRLSIFQGSWTQDAIGIICSGNGIASHGISDL
                     FTQLINKSLIEAIERSTDKEPRFRMLETIRQYAYEKLLETEGSETIRQKHLAYFMGLV
                     ERAAPNLRTFDMLIWLDRLEDELDNIRAALEWGLKSDIETVLRLASALLWFWHIHSHQ
                     NEATDWLKQGLAREANERGGNPIGPQRALLRGNALNTISFLMRNSEQRITLLEESLSL
                     FRELGVQGRQGLATALRILGNSELEKGHNKQARAFVEEGLSIFMEIREPFDMAECLDL
                     LVSLAMDEGDFERARGLQEEALSLRKEIGDKDGTAYALYILGQIAHRQNKAALAQALY
                     EESLKLFREIDNRTWVADITGTFAVLASAQGNRKKTNLLIEEGLALARDLDDKYGLAH
                     LMLNLGEAMRFQGDLDRASQLYEEALVLSQRMDANNFISSVLYGKAEIARSQNDLEQA
                     YALHCEALNIRQKISHTYGIVRSLNAIAELTILQGNSPQAVRLFGAIESMYKTSRPPT
                     EQAGHDRVVSAARSQMGETMFNSIWSEGQKLPLDQAISLVLTKRSAT"
     gene            6609..6995
                     /locus_tag="PL000_17130"
     CDS             6609..6995
                     /locus_tag="PL000_17130"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNC90655.1"
                     /translation="MPLIPQTCPSCSSPLVVTQLNCTSCGTGVVGKFELSPFSRLSPE
                     RLHFLEIFVRNRGNVKEMERETGESYWAIRRQLDEVITEMGFEETPKEDDPSTRRQEI
                     LARLSRGEINVQEATKLLAQIKGDPA"
     gene            6992..8353
                     /locus_tag="PL000_17135"
     CDS             6992..8353
                     /locus_tag="PL000_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90656.1"
                     /translation="MNGTKERLMILDMIAEGKITAAEGEELFKVLEEGDEPSAEPDFP
                     MPGPPSRPFPPLRPEPPQSPRGQRASTELVAALKTAGIDHVTLSDIQEMQDHNLTAEY
                     VHEMLALGIEPDGLGEWINLRMHDITPRYVRELRDMGVDDLDMDELIELSLHDVSAKY
                     VSELREVGLKDLDVDELVELSNHEVSAKYIAEMHELGLKDLDVDELIELNDHEVSARY
                     VAELRAMGLKDFDVDDLIELSNHEISPKYVAELKKMGFKNFDVDDLVELGVHEISPEF
                     IAELQTLGIKDLDIDDLVELGTHGVSPEFIAQMRESGLKNLDTDDLVQLSIHNIDPEF
                     MKALRDFGITGFDVDDVVELGIHNVTARYLTEMKEAGLKDVDTEELVEMRIHNVTPKF
                     VRELREMGFSNLPADELVELSIHHVTPRFIREMRQRYGENLTLEQLLDMRLHGVEEAL
                     GSR"
     gene            complement(8430..9404)
                     /gene="pfkA"
                     /locus_tag="PL000_17140"
     CDS             complement(8430..9404)
                     /gene="pfkA"
                     /locus_tag="PL000_17140"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNC90657.1"
                     /translation="MADKFTVGVLTSGGDAPGMNAAIRAVVRTALTNNMKVIGVHNGY
                     EGLINGEYHQLDARAVGGILQRGGTILLTSRSKRFQEPTGQRDAIRKMNEAGMDALIV
                     IGGEGSMNGAYALSQKGVKVIGIPGSIDNDIWGTNIAIGTDTAMNTIMEAVDKLRDTA
                     SSHQRAFVIETMGRNSGYLAVMAGIVCGAEMVLIPEVPVTGEEVAAAVEDAYQRGKTH
                     AIIINAEGSAIRTTDLVEKIDTLDLGFKTRMTILGHIQRGGSPTAYDRLLASRMGVKA
                     VEALVEGQHGVMTGLKGKGVDFIPLLDVISNKRKVNMEYYHMTKVLAR"
     gene            complement(9394..10326)
                     /locus_tag="PL000_17145"
     CDS             complement(9394..10326)
                     /locus_tag="PL000_17145"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNC90658.1"
                     /translation="MFEILFLGTSASAPSAKRGLSGQIVSHNEYRFLVDCGEGTQRQI
                     LQSGIGFKRLTRILLTHGHLDHILGLGGLLSTFLRWEAIEELEIYAGHSTLERVRTLL
                     YDVVLRGNQPPMPLILKEIKPGVIFEAEDFTVTAFSVTHRGPDCLGYIFEEKPRRPFL
                     SQKADELGVPFGPERRDLVEGREITLANGTRIKPDEVLGDWEKGSKLVLVGDAGRTDN
                     LLEVCKDADALVIESTYLDEEADMAKQFSHLTARMGAELAQKAGVKKLILTHISRRYR
                     EKDVIAEAQSIFANTVVARDFDNYQIRKETEHGR"
     gene            10625..12952
                     /locus_tag="PL000_17150"
     CDS             10625..12952
                     /locus_tag="PL000_17150"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HNC90659.1"
                     /translation="MPLLKPSTPPKKTAKPAGKTIPPKKGEAPQNHPAQKGKGQSPAP
                     TPPPPPTPALSAWDRLSPERKLDVVGIILAFFGVIIILGLASANRSAVVGGIIFFIAQ
                     IFGWGVYILPLGLLIFGLWLVFRKIERIPPLSIERVVGSVILFLWLLIVLHTFVATAE
                     TAEAEALKGIGGGYIGGKLQWFLWAGFGGGGAFIALVAWLVIGIAVLLDKPVQDLFFW
                     LGPIFTWIRNLLNKPIASDSPAPASASTEDLTPIDPSAMPDVKPAAGIGQPTRTTSGA
                     TVIHWTLPNVSEILDAGNAPSINEDFIQQRARLIEETLASFGAPAQVVAISRGPSITQ
                     FGVEPLFLESRGGVKTKVRVSKIASLSDDLALALAAPRIRIQAPVPGHSYVGIEVPNE
                     EMAMVALRDILEGEVYKRNTKPLNFALGRDVAGLPVIASIESMPHLLIAGTTGSGKSV
                     CVNSILTCMLLYNTPDDLRLVLVDPKRVELTGYNGIPHLLGPVVVEMDRVIGALQWMT
                     REMDKRYHMFAQVGSRNITDYNAKMKLKGEKKLPFLVVIIDELADLMMIAPDETEKTI
                     TRLAQLARATGIHMILATQRPSVDVVTGLIKANFPARIAFAVASNTDSRVILDQPGAE
                     RLLGRGDMLYQAPDAPSAARLQGVYVSDHEIQSLVEYWRNQAGGASPYAVAGSNPADA
                     VPTNVPLKQAPLWEDMTKVEGDPLFDDAVEIVRKEGRASVSMLQRRLRIGYTRASRIV
                     DTMEDKKIVGPPEGATQMRAVLDYGQTAPPKDDGM"
BASE COUNT         3003 a         3408 c         3414 g         3232 t
ORIGIN      
        1 ttttttgttt aaacgctaaa ataggggtat gcaaaaatat ctgcccgtcc tgcgtgcgcg
       61 gcgtgaacgc agactacaaa agcaaaacaa acaggcgaat aatgcgcgtg gcgcgattct
      121 cagcgggggg atcgttttat cgctgctgtt gggtgtgttc atcattttgg gtgcgtttgc
      181 gtatgccgat gtgacgcgtg aattgccttc cgtggaaatc cttccgcgtt tgcttaaccc
      241 gcctgatggg cttttgctcg aacccaccca tatctacgac cgcacaggca cgcacatcct
      301 ttttacgttt gcacccgtgg acgaacaacg gcgttatatt cccgccaacc cgcagaatcc
      361 ccagcatatc cctgccaatt taatagatgc catcatcgca gtgcaagacc cgcagtttcg
      421 aaaacattcg gggtattccc tgcaagggct ggacgatgcg aatcagcacc cgaccattgc
      481 gcaaaaactg atcggcgggc tggtcattta caacgaacca ccctcactgc ggcgtgccat
      541 tcgtgagcgg atccttgcgg cgcagatcac ggcacagttt gggcgcacac aggtgatgga
      601 gtggtatctc aacagcgcgc actttggcaa ttatgcctac ggagtcgaag ccgcttcaca
      661 gttgtatttt ggcaaggcgg tggagcagtt gactctggct gagtctgcga tcctggcggc
      721 ggcaagcgag tctccggcgc tcaatccgct ggatgctccg caagtggctt tacaacgcgg
      781 gcgtgaagtg ctttatgtgt tgaacggact cggcattgtc agcgatgaag caacggaaat
      841 tgccctggca gaagagcctg agatccgtgc ttcgactgag gatgatgcga atcaacccga
      901 ccctgaacct gctccggctt tcgtgaacat ggtcctcgcc cagttggata cccagcttcc
      961 gcgtgaccgt gtggagcgcg gcggaatcaa catcatcacc acgctggatt atgacctgca
     1021 acagcaagcg gcttgtgtca ctgaagtgta tgcagtgcgc atggctgggt tggaagagcc
     1081 gaaagaggcc tgtgatgccg cgcgatttct gccttcactg ccgcctgcag tgacgctgac
     1141 agattcatct gcaagtgcgc tgatcctcga cccgaatcat ggccaggtgt tggcggtggt
     1201 gggggaaact gttcaagggc aggaaacgcc gctcgtgacc gcacacagcc ccgggtccag
     1261 tttggatgcg tttgtttatt tgacaggatt cacccgcggg ttgagtcctg ctacattggc
     1321 gtgggacatc ccgggcgaag gcatcatcca gaattttgat ggcgaatatc acggaccggt
     1381 gcggctgcgg cttgcgctgg cgaacgatta tcaagtgccg atggaggcat tgaaggtgca
     1441 aatgggggtt gagaatgtga gcgggattgc gtcgtctttt ggtgttgctc tggataagca
     1501 ggtgacgatg ctgcaattgg cgggtgcgta tggtgtgttt ggcaagcagg gtgtttattt
     1561 tggccagaac atgaacgatg aatttctgcc ttccgctgtg ctgcgtgtgg aaggtgtgga
     1621 tcgttcgctg ttgttggatt ggtctctgcc gcaggcgaag cctgtgttga cccctgcatt
     1681 ggcctatttg atgacccatg ttttgagtga tgaggcttca cgctggccat cgttgggcag
     1741 gccaaatgtg ttggagatcg gcaggcctgc cggtgtgaag gtcggtcaat cagccaatgg
     1801 attggatgcg tgggtggtgg gatatacccc tgctcatgtg gtggtgacct ggaccggggt
     1861 gcatggtgaa gcggagtcgg ttactccgcg cttccctgct gcgttatgga gcgccttgat
     1921 gaaagttgct tcgggtgatg ctcctgctga tggttggacc gcgccgcagg gagtttcgac
     1981 catcacagtg tgtgacccat cgggtatgct gcctacaaag gaatgtccgc gtttggtgag
     2041 tgaggttttt ctgaatggca gtgaacctgt gcaggctgat accctgtacc gtgagtttgg
     2101 gatcaaccgt gaaacgggtc tgcttgccac cgtatttaca ccgcccgaat tgatcgaaac
     2161 ccgcatttac atgctggtcc ctgaatcagc acgtgactgg gccttgagcg ccgggttgga
     2221 gattccgccc gcttcttatg atgccattca ggcgccgccg gtgaatccgt tcgcgaatat
     2281 tgtggaacca aaattgtttg ccgaggtcgg cggcgttgtg aagatcatgg ggaccgcggc
     2341 tggggagaat tttcagtatt atcgcgtgca ggttgggaag ggtttgaatc cgcaggaatg
     2401 ggttcaactt ggcggcgatg tgatttcgcc tgttgaagat ggcctgcttg ccgaatggga
     2461 taccagcggt ttgagcggtt tgtatgctgt gcagctgctg gtggtgaggg cagatcagat
     2521 cgtggataca gcagtgattc aggtgacgat caagtagctg atcgttttgt cagtacgaga
     2581 gagatcgcct gatccagagg taatttttgc ccctcgctcc agatcgagtt gaacatggtt
     2641 tcgcccattt gagatcgtgc agcggagacc acacggtcat gcccggcttg ttctgtagga
     2701 gggcggcttg ttttatacat ggattcgatc gcaccaaata atcgaacggc ttgtggggaa
     2761 ttcccttgaa ggatggtcaa ttcggcaatt gcgttcagcg agcggacgat tccgtatgtg
     2821 tggctgatct tctgcctgat gttcagtgct tcacagtgca gggcgtatgc ttgttctaaa
     2881 tcgttttgag aacgggcgat ttcggctttg ccatacagca cggatgaaat gaagttgttg
     2941 gcatccattc tttggcttag aaccagggct tcttcataaa gctgcgaggc ccggtcaagg
     3001 tcaccttgaa aacgcatggc ttctcccaga ttcagcatta agtgagccag tccgtatttg
     3061 tcatccaggt ctctggctag agccagacct tcttcgatca agagatttgt ttttttgcgg
     3121 tttccctgtg ctgaagccag aacggcaaag gtgcctgtga tgtcagccac ccatgttcgg
     3181 ttatctatct cgcggaatag tttcaggctt tcttcataaa gggcctgtgc cagtgcggct
     3241 ttgttttgtc tgtgagcaat ctggcccaaa atgtagagtg cataggcggt tccatccttg
     3301 tcgccgatct cttttcgtaa tgacagggct tcttcctgaa gtcctctggc gcgttcaaag
     3361 tcgccttcat ccattgccag gctgaccaat aaatcaagac attcagccat gtcgaatggt
     3421 tctcttatct ccataaaaat ggacaggccc tcttccacaa atgcacgtgc ctgtttgttg
     3481 tgcccctttt ccaactcact atttcccagg atccgtagtg cagtggcaag tccctgcctg
     3541 ccttggacgc ccaattcacg gaacaatgat agggattctt ccaataatgt gatccgctgc
     3601 tcggaatttc tcattaggaa actgatcgta tttaaggcgt tgccccgtaa caaggcacgc
     3661 tgcggaccta tgggattccc gccgcgttcg tttgcttcgc gtgcaagacc ctgctttagc
     3721 cagtcggtgg cttcgttttg gtggctatga atatgccaga accacaataa cgcacttgcc
     3781 aatcgtaaaa cggtttcaat gtcacttttc aatccccatt ccagtgcggc gcggatattg
     3841 tccaattcat cttccaaccg atccagccag atcagcatat cgaatgtccg taaattcggc
     3901 gctgctcgtt ccaccaaacc cataaaatag gccaggtgtt tttggcggat ggtttcactg
     3961 ccttccgttt ccaataattt ttcatacgca tattgacgga tggtttccag catgcggaag
     4021 cggggttctt tgtcagtgga ccgttcgatg gcttctatca gtgatttgtt gatgagttgg
     4081 gtgaataagt ccgaaatgcc gtgtgatgca attccatttc ctgagcatat gatcccgatc
     4141 gcatcctgag tccagcttcc ctggaagatg gagaggcggc aaaataactt ccgctcattt
     4201 tctgagaggc tgtcataact ccagtcgatc agggcgcgca gggtttgctg acgcggcgct
     4261 gctgcgcggt tgccgcgtgt caacaggttg aaacggtcgt tcaaccccgt ggcaatttcc
     4321 tcgagcgtta aagttctcaa ccgtgcggcg gccagttcaa ttgcaagcgg aatcccatcc
     4381 agttgcctgc aaatttgggc aatgagaggc gcttcttctg cattgaccct gaacgtggaa
     4441 agggcaaagg cggcgcgctc tgcaaaaagc cgtgctgcgg cgtactgcat taattgtccg
     4501 gcaggaacct cggcttgtat atttggggtt tccaacgcag gcatgcgata taccagctct
     4561 cctgcaatgt cgagcgcctc cctgctgctt gcaagaatgc gtacactcgg actggtgctc
     4621 aatacctcat gggaaaattg tgcgcacatg tcaatcagat gttcacaatt atcgaggatg
     4681 aggagaattt ccttgtcact gagccaggtg catacggtct ccattaaaga atgggattgc
     4741 acctgttcca ctcccaatac cgtggcaatg gtgtacaacg ccagtgcagg gtcggtgacg
     4801 ggggcaagtt caacgagcca aaccccattc cgaaaaccat ttattgcttc tttcgccact
     4861 tgcagcgtca gacgggtttt gcccattccg cccgggccaa ccaccgttaa gagtcgagtc
     4921 gttgccagta gttgacgaac atcttttagc tcagtttcgc gtcccacaaa actggtgagt
     4981 gcggccggta aattgttcgg gctgacacgc ttttgccatt gccgcattcc tgctgcgccg
     5041 atggttgcat agttttcact gagatgccat cccgaatcct gccttgttag tgcaccctct
     5101 ttcaacatgt gtttcaaaac ctgctcagct tgagcgggta aaccatgggt atgtttttcc
     5161 agccaatgga tgaaatcgtt gggcgggtcc cattgaagca atgtccgcag ccacacttgc
     5221 aaacctgcag gcgtgagcgg ggacaaggtc acatactctg acagagccgc ccgaaacggt
     5281 gcggtgaccc tggcatggtc tgggtcggtg gcgtagacca atcccaacac cgggaattca
     5341 tttgaggaaa gaatctggcg caggaggtcc aatgtgcccc aatcaagctg gggtaggtca
     5401 tccacactga tcagcaaacg gtcacgtcct gattcggtga gtcggcgctg aaaagctttg
     5461 atgatttttt cgagattgtt ttgagtgaat gcggtgccgt cagggttttg cagtatgttt
     5521 tccagtacgc cataaggcct ggccattaat tggggatttc cgcgcagggc aatgacttca
     5581 aaaccttgca gtcgcgcccg tttttccact tcaacgagaa aacgggaaca tcccacacca
     5641 tttggaccaa tgacgccgaa tacgccgtgt tgttgactca ctaaggcaga taaaaagttt
     5701 tgtgccagtt ccagactggc gtcacgttcg ataaggcgcg atggctttat aaatgacaaa
     5761 tcagatgttg tgcccccatc cctgcttatt acacatgccc gtccgcgccg cttggcttcg
     5821 taattgcgcg catctgcttt ttcaaatagc gattgaccgt ccgatgcatc ttcagggaag
     5881 gcagctacgc caatacttaa tgtcagggtc agtactgtat ccctgctaaa agactgggca
     5941 cgtatgattc ccagtaagcg ttgtgccaga tcgaacgctt ctgtctttgt tgtgtttggc
     6001 agcaataaaa caaattcatc accgccatac cgaaaaaaaa gatcggacgc ccgcgtattt
     6061 tgcaggatga gatgagcggc cccacttaat acctcgtccc cttttgcatg gccaaatgca
     6121 tcattgatgc ttttgaaatg atcaaggtca atcaccatta aagaacatgt tgtgttatca
     6181 cgctgcgcct gttcaatgag ggcatgtaaa cttgccttga atgttgcacg tgtatatgcg
     6241 ccggttagag tatcctgggc gggtgcttca ttcaaggcat tgtattcgtt gggttctgat
     6301 gacatcggtg ggtattccat aagacaagcc gtcttcagaa aaaggcgaac agctttcatt
     6361 atatggcaaa atcaatccgg acattcagtc tcctgaaaga tccttcccaa ggttacaaat
     6421 cgttgtttac cgtgggtctt tcctgcgtgg atgcccattt tggcgtgtaa aaccctaatt
     6481 tgagtaaaaa aacggcgcaa aagccgtaaa acaaaataaa taggcgtaaa accaaaatat
     6541 tttgaagtaa accaaaaata ttgacaaatc caaaaatatt tgctaatata tcgccaggag
     6601 ctccccctat gccattaatt ccccaaacct gcccaagttg ttcgtctccg ctggtggtta
     6661 cccaactcaa ctgcacatca tgtggtaccg gcgtagtcgg caaattcgaa ctttcgccat
     6721 tctcccgtct ctcgccggaa cgcctgcact ttttagagat ctttgtgcgc aaccgcggca
     6781 atgtcaaaga gatggaacgc gaaacaggcg aatcctattg ggcgatccgc cgccaacttg
     6841 atgaagtcat cactgaaatg ggttttgaag aaacacccaa agaagatgac ccatccaccc
     6901 gacggcagga aatccttgca cggctgagtc gtggcgagat caacgtgcag gaagccacca
     6961 aactgcttgc tcaaattaaa ggagacccag catgaacgga accaaagaac gcctgatgat
     7021 tttagatatg atcgctgaag gcaagatcac agccgccgag ggcgaggaac tttttaaagt
     7081 gttggaggag ggcgatgaac cttcggccga accagacttc ccgatgcctg gtccgccctc
     7141 aaggccattt ccgccccttc ggcctgaacc tccccaatcc ccgcgcggac agcgtgcttc
     7201 aaccgaactg gtcgctgcac tcaaaactgc aggcattgac catgtcacct tgagcgatat
     7261 tcaggaaatg caagaccata accttactgc cgagtatgtt catgaaatgc tggcccttgg
     7321 tattgaacca gacggccttg gcgagtggat caacctccgc atgcatgaca tcaccccccg
     7381 ctacgtccgt gagttgcgcg atatgggtgt tgatgacctg gatatggatg aactgatcga
     7441 actcagcctt catgatgtat ctgcaaaata tgtttctgag ttacgcgagg tcggcttgaa
     7501 agacctcgat gtggacgaac tggttgaact tagcaatcac gaggtctcag ccaaatacat
     7561 tgccgaaatg cacgaactgg gattaaaaga cctggatgtg gatgaactca tcgagttgaa
     7621 cgatcatgaa gtttctgcca gatatgtcgc cgaattacgt gccatgggtt tgaaagattt
     7681 tgacgtggac gacctgattg aactcagtaa ccacgagatc tcccccaaat atgttgccga
     7741 gttaaagaag atgggcttca agaatttcga cgtggatgat ctggtggaac ttggcgtcca
     7801 tgagatatca cctgagttca tcgcagagct gcaaacactg ggaatcaaag atctggatat
     7861 cgatgacctc gttgaattgg gcacacatgg tgtttcaccc gaattcattg cacagatgcg
     7921 agaatctgga ttgaagaatc tggatacgga tgaccttgtc caattaagca tccacaacat
     7981 tgatccggaa ttcatgaagg ccctgcgtga ttttggcata acgggtttcg atgtggatga
     8041 tgtggtcgag ctgggcattc acaacgtcac tgcgcgctat cttactgaga tgaaggaagc
     8101 gggtttgaaa gatgtggata ccgaggaatt ggtcgagatg cgcattcaca acgtcacccc
     8161 gaagttcgtg cgtgaactgc gcgaaatggg tttcagcaac ctgcccgccg atgaattggt
     8221 tgaattgagc attcaccatg tcaccccgcg ctttatccgc gaaatgcgcc agcgatatgg
     8281 agaaaacctt acccttgagc agctgctcga tatgcggctg catggagtgg aagaagcact
     8341 gggcagccgc taactttgca gaaagcctca taaacgaaaa ggccgttgag ataacttctc
     8401 aacggccttt tcgtattctt caattttcac tatctcgcca gcaccttggt catgtgatag
     8461 tactccatat tgaccttgcg cttgttgctg atcacatcca aaagtggaat aaaatccacg
     8521 cctttgcctt tcagtccggt cattacgcca tgttgacctt ccacaagcgc ctccacagct
     8581 ttgacaccca tgcgtgaggc cagcaaacgg tcataggcgg ttggtgaacc tccgcgttga
     8641 atatggccga gaatggtcat gcgggttttg aagccgagat cgagggtatc gatcttttcc
     8701 accaggtcag tggtgcggat ggctgaccct tccgcattga tgatgatcgc gtgtgtctta
     8761 ccgcgttgat aggcatcttc cactgcggcg gccacttctt caccggttac tggtacttcg
     8821 gggatcaaca ccatttctgc accgcaaacg attcccgcca tgacggcaag gtaaccgctg
     8881 ttacgcccca tggtctcgat tacgaaagcg cgttgatggg aggaggcggt atcacgcaat
     8941 ttgtccacgg cttccataat ggtgttcatg gcggtgtcgg taccgatggc aatgttcgtg
     9001 ccccagatat cgttatcaat actgccgggg atgccgatca ctttcacacc tttttgcgag
     9061 agcgcatacg cgccgttcat ggagccttcg ccgccgatca cgatcaatgc atccatgcca
     9121 gcttcgttca tcttgcggat cgcatcgcgc tggcctgtcg gttcctgaaa ccgtttgctg
     9181 cggctggtga gcaaaatggt cccgccgcgc tgtaagatcc cgccgactgc acgtgcatcc
     9241 agttgatgat actcgccatt gatcaagcct tcgtatccat tatgaacacc gatgactttc
     9301 atgttattgg ttaacgcggt ccgcaccacg gcgcggatcg cggcattcat gccgggggca
     9361 tcgcctccgg aagtaagtac ccctactgta aatttatctg ccatgttctg tttctttcct
     9421 gatttgataa ttgtcaaaat cgcgggccac caccgtattg gcaaaaatgg attgcgcttc
     9481 ggcgatcaca tctttttcac gatagcggcg cgaaatatgg gtaagaatta gttttttaac
     9541 ccctgctttt tgagcgagtt ccgcacccat gcgtgcggtt agatgggaaa attgctttgc
     9601 catgtctgct tcttcatcca gatatgtcga ttcgatcacc agggcatcgg catccttgca
     9661 gacttcgagc agattgtcgg ttcttccagc atcccctaca aggacaagtt tactgccctt
     9721 ttcccagtca cccagcacct cgtcaggttt gatgcgggtg ccgttggcga gggtgatctc
     9781 cctgccttcc accagatcac ggcgctccgg tccaaatggg actccgagtt catccgcttt
     9841 ttgtgagagg aagggacggc gcggcttttc ttcgaaaata tacccaaggc aatccgggcc
     9901 gcggtgggtc accgaaaacg cagtgaccgt gaaatcttcg gcttcgaaaa tgaccccggg
     9961 tttgatctct ttaaggatca atggcatcgg cggctggttg ccgcgcaaga ccacatcata
    10021 caaaagtgtg cgcacacgtt cgagcgtact gtgcccggca tagatctcaa gttcctcaat
    10081 ggcttcccag cgcaaaaaag tggaaagcag cccgcccagc ccaaggatgt gatcgaggtg
    10141 gccatgagtc agcaaaatac gggtcagtcg tttaaaaccg atgcccgatt gaaggatttg
    10201 tcgttgcgtg ccctcaccgc aatcaacaag aaaacgatat tcattatggg aaacgatctg
    10261 ccctgaaagt ccgcgttttg cagaaggcgc ggaggcagat gtgccaagaa aaagtatttc
    10321 gaacaaggtg aattcctcag gaaatgcttg aaaacccggg ggaacgggaa tcggaaagat
    10381 gcaatgagaa acggcgtaaa accccgcaat tgtaccttaa agaaaagacc atctcccgcc
    10441 tgaagaaaaa atcgatagta tttgaaagcg cagcgcacaa ccagtcgctg tgctgcgaag
    10501 ataacagacc atttgtgcta taatcttttt taacttcaac aaatataaac atcatatgat
    10561 gttgggcaga aaaaaggatg cgaatcattt tcatccttca tcctgtctcc ttcatctttt
    10621 tcatatgcca ctcctgaaac catccacccc tcccaagaag acggcaaaac cagcgggcaa
    10681 aaccattcct cccaaaaaag gggaagcgcc gcagaatcac cccgcccaaa aagggaaggg
    10741 acaatctcct gcgcctacac cacccccgcc cccaacaccc gccctctccg catgggatag
    10801 gctttccccc gagcgcaaac tggatgtggt tgggatcatc ctcgctttct ttggcgtgat
    10861 catcattctg gggcttgcct ctgccaatcg ctcagcggtt gttggtggca tcattttttt
    10921 cattgcccaa attttcggct ggggagtcta catccttcca cttggcctgc tcatctttgg
    10981 tctgtggctg gtcttccgca agatcgaacg catccctcca ctttcaattg aacgtgtggt
    11041 gggcagcgtc atcctctttt tatggctgct catcgttctg catactttcg tcgccacagc
    11101 agaaaccgct gaagccgaag ccctcaaagg tatcggtggt ggatacatcg gtggaaaact
    11161 ccaatggttt ctttgggctg ggtttggcgg cggcggcgcc ttcattgcac ttgttgcctg
    11221 gctggtcatt ggcattgccg tcttgcttga taaacccgta caggatttat tcttctggct
    11281 cggaccgatc ttcacctgga ttcggaacct gctgaacaaa cccatcgcct ccgactcacc
    11341 cgcgcctgct tctgcttcga cagaagacct caccccgata gacccctccg ccatgcccga
    11401 tgtgaaaccc gccgcgggga ttggccaacc cacccgtacc accagcggcg ccaccgtcat
    11461 ccattggact ctgccaaacg tcagcgaaat tcttgatgca ggcaacgcac cctccattaa
    11521 tgaagatttc attcaacaac gcgcaagatt gatcgaagaa acactcgcct cttttggcgc
    11581 accggcacag gtggtcgcca tcagccgtgg accttcgatc acccaattcg gcgtggaacc
    11641 gcttttcctt gagtccagag gcggggtaaa gaccaaggtg cgtgtcagca agatcgcctc
    11701 cctttccgat gacctggccc ttgcgctggc cgcccctcgc atccgcattc aagcgccggt
    11761 gcctggtcac agttatgtcg gcattgaagt tcccaacgaa gaaatggcaa tggttgccct
    11821 gcgggatatt ctcgaaggcg aagtatacaa acgcaacacc aaacccctaa actttgcact
    11881 cggacgagat gtggctggtc tgccggtcat tgccagtatt gagagcatgc cgcacttgtt
    11941 gatcgcaggc accaccggct cgggtaaatc ggtctgtgta aattccatcc tcacctgcat
    12001 gttgctctac aacacccccg atgacctgcg cctcgtattg gtggacccca aacgcgtgga
    12061 gttgaccggc tacaacggca ttccgcacct gcttggcccg gtcgtcgttg aaatggatcg
    12121 cgtgatcgga gccttgcaat ggatgacacg cgaaatggac aagcgctatc acatgttcgc
    12181 gcaggtgggt tctcgcaaca tcaccgatta caacgccaaa atgaaattaa aaggcgaaaa
    12241 gaaactaccc ttcctggtgg ttatcattga cgaactagcc gacctgatga tgatcgctcc
    12301 tgacgagacc gaaaagacca tcacgcgcct cgcacaattg gcccgtgcca caggcattca
    12361 tatgattctc gccacccaac gtccgtctgt agatgtggtg acaggtctca tcaaagccaa
    12421 cttcccggcc cgcattgctt ttgccgtggc ctccaatact gacagccgtg tgatcctcga
    12481 ccagcccggc gctgaacgct tgctcggccg tggtgacatg ctctatcagg cgcctgatgc
    12541 tccctctgcc gcacgtttac aaggcgtcta tgtttcagat catgaaattc aaagcctggt
    12601 ggaatactgg cgcaatcagg ctggcggggc gagtccgtat gctgtggctg gttcgaaccc
    12661 tgccgatgca gtgccgacca acgtaccgct caaacaggca cctctttggg aagatatgac
    12721 caaagttgaa ggcgacccgc ttttcgatga tgccgttgaa atcgtccgca aggaaggccg
    12781 cgcatctgta tccatgctgc agcgcaggct gcgcatcggc tatacccgcg cctcccgcat
    12841 cgtcgacaca atggaagata aaaagattgt cggcccgccc gaaggtgcca cccaaatgcg
    12901 agccgtgctt gattatggtc aaaccgcacc cccaaaggat gacggaatgt agaacagaac
    12961 gagagcgaca ttcaatgtcg ctcttttatt gcctgatatc gggcaactca tcaggcactg
    13021 cctgtcatca ggtaataact gggagtggct aaaagga
//
LOCUS       DAOQLC010000121        12684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1878_length_12684_cov_21.697601, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000121 DAOQLC010000000
VERSION     DAOQLC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1878_length_12684_cov_21.697601"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2273)
                     /locus_tag="PL000_17155"
     CDS             complement(<1..2273)
                     /locus_tag="PL000_17155"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNC90660.1"
                     /translation="MNFQYSPVAFVLLITALISIFIAFYIWPQRKTRGVTALIILSLS
                     AAQWLIFYALEVSAIDLETKFFWGKLEYVGIVLAPLSWLLFSLDYAGLERFLGRRAIV
                     VYSIIPAITIALAVTTEYHGLIWSEYHIEYLANFSALGVTHGLWFWVHFSYSYIILLI
                     GTALLIRVFLSRQEGMYRSQGVALLVASLAPWIGNALYLTGNSPIPNLDPTPFAFTIS
                     IAALAWAIFGLHLADITPLARDLVIDSMREGMIVLDARNNVIDINTAASLMIGVSIPD
                     AIGKTAADVFSPWPHLIEKFRSAVDVNQEIAVGTGDSQRRYQVRFSALTGQQDELLGR
                     VIMLRSLDESLVLAPKVQTEKSASRSMLEADTQPQADSPRNIFSLLYNFFFAPAKTDQ
                     KAPANMNPGWFQARERSFTIITRVSALIGSIVFIFVFFFMDDAELQRVNLIYGVAVVL
                     LWSFGLLRTLDFNYRAFLFLLMVYALAVVETINYGFSVESFIFFTAFVVTAVVLIGRS
                     GGWMAAAISLFSLFIFNWLIGTRYFIPVNLTLETVVPPDFGSGLTSLLVFSASSLAMV
                     SSITILMESLNRAWQSELQSSNLLQQERDLLEQRVEERTQDLEDARDSAVRSNIELRK
                     YYRVIEQSGNSIVITDLDGNIQYANPRFEEVSGYALEEVLGKNPRILKSGRQKPEFYE
                     KMWRTISSGHIWTGVFHNRRKDGTLYWESATIAPVLDQDNVVVNYVAIKEDITSRREV
                     ESQLRKLSRAVEQSGNTV"
     assembly_gap    2405..2504
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2506..2892)
                     /locus_tag="PL000_17160"
     CDS             complement(2506..2892)
                     /locus_tag="PL000_17160"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC90661.1"
                     /translation="MQNQRKESRKKLIAFTPVYELLHKKLIGYVGDLTLQGVMVIGET
                     SLEAGRRLAIGIEFPADELDEKLQRVAVSGRVAWCRPDEDTPQYLNIGFEFLELSPEG
                     AEIIKGVLDRYQFRYIMDISELKRKS"
     gene            complement(2906..4153)
                     /locus_tag="PL000_17165"
     CDS             complement(2906..4153)
                     /locus_tag="PL000_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNC90662.1"
                     /translation="MPEFLTLLPPDEARDKLIAHLSAPEFDSDSIDILHALGRVTAED
                     ILAPHPLPDFQRTTVDGYAVRARDTFGATDSLPAYLNLIGEVPMGDAPSFEIGTGQCA
                     LIHTGGMLPNGADAVVMLEYTQHIDGAGEIEVFKSVADGENIIRIGEDVAQGQVVIPR
                     GTILRPAEIGGLTALGFTSLRVARKVMVGLISTGDEVIEPNQTPRPGQVRDINSYTLG
                     ALVEKAGGATKRYGIIADQFSALKEAAAQALSECDVVVITAGSSASTRDMTAEVIRQL
                     GEPGVLVHGINTRPGKPTILGVCNGKAVIGLPGNPVSALVNGYLFVVPVIEKLLGMLP
                     KPKASVRAMLTVNLPSQAGREDWWPVKLIAEQPASNVKYKAEPIFGKSNLIFTLAAAD
                     GLLRIHPDATGLSAGEVVEVVLI"
     gene            complement(4157..>4998)
                     /locus_tag="PL000_17170"
     CDS             complement(4157..>4998)
                     /locus_tag="PL000_17170"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNC90663.1"
                     /translation="LLLLTALAYLLGASISAYLGKSFQNLAFLLGLGYILLAQLSMNL
                     LAEAFRPHNEPMIEGETPAQKEVLRSNLLYISYAALTACAMIAFLLYFNHSLNLFSFL
                     FLLLSFVLVVLYAVPPLRFLERGFGELALAVHMAYVAPSIGFLLQAKENHRLLIIIAL
                     PLTALALAYFLILNFTSFPSDQKYQRATLLRRLGWETAVPLHHSLIIFAYVIFLCSPI
                     FGYSFLLIWPAFLTLPFAILQMLLLRHISLGGKPNWPLLTTTALAVFGLTAYVLTLTF
                     WMR"
     assembly_gap    4999..5098
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5099..6193)
                     /locus_tag="PL000_17175"
     CDS             complement(<5099..6193)
                     /locus_tag="PL000_17175"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC90664.1"
                     /translation="MILPPFKLERYFAKYEFTTEYLLCSSDCEAMSIAELLALEPGSA
                     EKFQNVWLGYTESLGSPSLRKEITNLYTTMQPDDILVHTGAGEAIYLFMHAAFKKGDH
                     VIVHSPGYQSLAEVARAVGCDVSPWRAREQDGWALDLDELRHLMRTNTKGIIINTPHN
                     PTGYLMSHADFEAVNKFAQEKGLILFSDEVYRESEYDSADRLPAACDMGEHAVSLGVT
                     SKTYGLAGLRIGWVATKNKKILQAMASLKDYTTICNSAPSEFLAEVAMRNRQILVDRS
                     LGIIKHNLTVVDDLFNRHAALFTWARPKAGSMGFPKLLKGDIEDFCDDLVRKAGVLLL
                     PGSVYDDSRNHFRLGLGRKNLPQAVERLEGY"
     assembly_gap    6262..6361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6471..6941)
                     /locus_tag="PL000_17180"
     CDS             complement(6471..6941)
                     /locus_tag="PL000_17180"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC90665.1"
                     /translation="MSETTELAEKLKTEGEKYIEFFSAMNESLWQQEVYTEGEMWTVR
                     NVLSHFVTSERGLVKLFEQIRQGGTGAADDFSIDRYNASQQAKTKDLSPSELLEQYKE
                     VRANSVKWVSGLKDEELEIKGRHPFLGETVLREMVKMLYIHNQLHFRDLKKALK"
     gene            complement(6969..8645)
                     /locus_tag="PL000_17185"
     CDS             complement(6969..8645)
                     /locus_tag="PL000_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90666.1"
                     /translation="MSFKKLPLDLLASIFLIAFTLFYAARFVDFSIPPFEDAAMLMRY
                     AQHLAEGHGIVWNIGEAPVDGATDFLFMAASAALIKLGFTVGQSVRGIGFVSHLLTVL
                     IVYWTNRRVNHTDIPFSLLSGLYLAVGTGFSYVAAYFGTPFFALAAASTWTLGLLLIQ
                     KENPPFSLILAFALSGLVTGLIRPEGVILASLMLLAIIVMRGWKNSVAIIVTFGSVFL
                     LLGGAYFVWRWNYFGYPLPNPFYKKGDSGLHWDSFYNSLSNTLRMCLPFVFVFILGFR
                     SRETSTRTLAYLIPILGFASAFILISDEMNFGARFQYALVPLVLMSWTPLLFSGNQSG
                     LGVLSSLYREVPVGGDQNQLSVRNGRVYLVAGIALFVSLVYYSSYQNCFLTSFQQTCA
                     RPYERDGRYEMGRLLAEYRGKGYVIATTEAGLVPYYSGWTAIDTWGLNDQFIAHNGKI
                     TVEYLDKYKPHIIMFHDYYSPLVPPKLTDANLAQRWFGMTILLKTYAEENGYILAAVF
                     GDSPYDTNYFYVRPDFEDSKRLVGQISGFKDYYWFTTGKRSINYAEFDKP"
     gene            complement(8712..9425)
                     /locus_tag="PL000_17190"
     CDS             complement(8712..9425)
                     /locus_tag="PL000_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC90667.1"
                     /translation="MPLALVTGGAHRLGKAFALSLARMGYSIALHYRASANEADHTVK
                     EIRALGVDCLPIRADLTLPEKIDFLFSLVDEFKTPLKVLVNSAAVMPVGKPQELEIHD
                     WDLALDLNLRAPFMLAQEAAKRMTDGGLIVNITDIGAQKAWSRYPSYTVSKAGLESLT
                     KLLARALAPTIRVNAIAPGLVLPSDVVTEEQWDNLVQKLPLKRAATLEEVTSALEFLI
                     KNEYITGQTIVVDGGYSLL"
     gene            9557..10417
                     /locus_tag="PL000_17195"
     CDS             9557..10417
                     /locus_tag="PL000_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90668.1"
                     /translation="MNNPIIAKELKGRMRGRQGFVMLTAYLGLISLVIYAVYVYSVRN
                     TYSRSPGFLQEAGKQVFLIVVLLEIILTGFIGPALTSGAISSERERQTYDQLRMSLLS
                     AKDIVIGKLGSSVMYLLLLIVAAIPLQSLAFFLGGVGIEEVLVSIWILTLSTINFCTL
                     GLYFSGISKRTSIATILSYTTLILPILLFAAGIYAINENSYLLSNASFEFQRWLLNVL
                     WTIISTNPASAAFVSEAMLIEEQNLFLIQSPVPPMVLVSPWILYSIFSITMTILMIRL
                     IVFYLKRHEP"
     gene            10432..12015
                     /locus_tag="PL000_17200"
     CDS             10432..12015
                     /locus_tag="PL000_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90669.1"
                     /translation="MSTINHLNHTLQNWIQWLRVQRALTWYLRGLAAALGLSLLFGGI
                     GLAQAKLLKQEFLALVVASVTLLPILSAIIAFFWKVNTTKAARHFDRAFHLEERISTA
                     LELSTADHANEMVQKQLDDAVSAAHRIQPRRDLPLRLKRFDSALTVILALLIGVLWFR
                     GDALFAAASRQRAVEQAIAAQQEQIEEIIKEINENEALTAEQKQELTEPLNQALQALD
                     QNPSLEGAVSILTNTGEQLQALNSEQAGQTAQALKEAGSSLQAQEGTPLSSVGQNLAS
                     GNFTQAANDLNNMDLSQMSPEELQKLADQLEATAQSLASTNPQLAQAMQQAADAIRNG
                     DLAAAQQALANASQQMANAGQQVTAAQTASQAAGQMQQGAGQLLAAGGGNQSAQANGQ
                     GQGQQGNNSGGQNSSAGSGSGSGDSPDSPQQGNEAGSSPIEQNNGAGDGGESTYEQIY
                     APSLLGGENGENVGLPTSGNDGDVVGTSPTTATDGQSLVPYTNVFTQYEQFYYQAIEN
                     GQVPMQFFDLIRNYFISLK"
     gene            12026..>12684
                     /locus_tag="PL000_17205"
     CDS             12026..>12684
                     /locus_tag="PL000_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002515251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC90670.1"
                     /translation="MSDFSVDTFREYANRIETEVGRVIVGQGDVVRHVLVSILAGGHV
                     LLEGVPGLGKTMLIRTLGQALDLKFSRIQFTPDLMPADILGTEILSDDNGKREFRFRE
                     GPLFSNLVLADEINRATPKTQSALLEAMQEASVTVAQRTYKLEEPFFVMATQNPLEME
                     GTYPLPEAQLDRFFFKVNVDLPNVGEMVEILDRTTGKESPAASKAVTGADIIQMRALA
                     RT"
BASE COUNT         3306 a         3194 c         3166 g         2718 t
ORIGIN      
        1 accgtgttac cactctgctc aaccgcacgg gaaagttttc tgagttggct ttccacctcg
       61 cgtcgtgaag tgatatcttc cttgatggca acatagttga caaccacgtt atcctgatca
      121 agcacaggag caatcgtcgc ggattcccag tacagggttc cgtctttacg gcgattgtgg
      181 aagaccccgg tccagatatg cccggaactg atcgtgcgcc acatcttttc gtaaaactct
      241 ggtttttgcc tgcccgattt caaaatccgt ggattctttc ccagcacctc ctccaaagcg
      301 tacccggaaa cctcttcgaa cctggggttg gcatattgaa tattgccatc gagatcggtg
      361 atgacaatgg aattgccgct ctgttcaata acacggtaat acttgcgcaa ttcaatattg
      421 ctgcgaacgg cgctatcacg tgcatcttcg aggtcttgcg tacgttcctc cacgcgttgt
      481 tcgagcaggt cacgttcctg ttgaaggaga ttggaagact gcaactcgga ttgccaggct
      541 cgattcaaac tttccatgag gatggtgatc gaggagacca ttgcaagtga acttgcagaa
      601 aaaaccagca aactcgtcaa ccccgagcca aaatcaggag gcacgacggt ttcaagcgtt
      661 aaattgacag gaataaaata ccttgttcca atcagccagt taaaaatgaa aagcgaaaaa
      721 aggctgatcg cggccgccat ccatccgccg ctacggccga tcaacaccac tgcggtcacc
      781 acgaaggctg taaaaaagat gaagctttcc acagaaaagc cgtaatttat ggtttccaca
      841 acagcaagcg catacaccat gagcaggaac aaaaacgccc tgtaattgaa atccagtgta
      901 cgaagaaggc caaacgacca aagcagtaca acggccaccc catagatcaa attcacccgc
      961 tgcaactccg catcatccat aaagaagaat acaaagatga atacgatact gccaatcaaa
     1021 gcggagactc tggtaatgat ggtaaaggaa cgctcgcggg cctggaacca gcccgggttc
     1081 atattggcag gcgctttctg gtcagttttt gcgggggcaa agaaaaaatt atagagcagg
     1141 ctgaagatgt tgcgggggct atctgcctgg ggttgggtat ccgcttccag catggagcga
     1201 gatgcggatt tttccgtctg taccttcggc gcgaggacta gagattcatc caaagaacgc
     1261 aacatgatga cacggccaag caattcatcc tgttgccccg tcagggcaga gaagcgcact
     1321 tgataacgac gctgcgagtc gcccgtgccg acagcgatct cctgattaac atccactgcg
     1381 ctcctgaatt tttcgatcag atgcggccat ggagagaata catctgcggc tgtctttcca
     1441 attgcatctg gaatgctcac gccgatcatc agggaggcag cggtattaat atcgatcaca
     1501 ttgttgcgtg catcaaggac gatcatcccc tcgcgcatgg agtcgatcac aagatcgcgc
     1561 gccaggggcg taatatctgc cagatgaaga ccaaaaatcg cccaagcaag ggcggcaatg
     1621 ctgatggtga atgcaaacgg cgtagggtcg agattgggaa tggggctgtt gcctgtaagg
     1681 taaagggcat tcccgatcca cggtgccagg ctggccacca ataatgcaac tccctgactt
     1741 cgatacatgc cttcctgtct gctcaaaaac acgcggatca gcaaagccgt accgatcagg
     1801 aggatgatat aagaatagga aaaatgcacc cagaaccaaa ggccgtgtgt cacccccagt
     1861 gctgaaaagt ttgccagata ttcgatgtga tactcggacc agatcaggcc atgatattcg
     1921 gtcgtgaccg ccaatgcaat tgtgatggct gggatgatcg aataaacgac gatggccctg
     1981 cggccaagaa aacgttccag acccgcataa tcaagcgaaa acaaaagcca ggaaagggga
     2041 gccagaacga taccaacgta ctccagtttc ccccaaaaga attttgtttc aaggtctatt
     2101 gcagaaactt caagtgcgta aaaaatgagc cattgagccg cacttaacga caggatgatc
     2161 agggctgtca caccacgggt tttacgctgc ggccaaatgt aaaaggcgat aaaaatcgag
     2221 ataagagccg taatcagcaa cacaaaggcg acgggagaat actgaaaatt catacacaac
     2281 ccttcaaatt tacaagctgt attttcgcaa ggaattcccg cattatacgc gaaggggtgg
     2341 cattacagaa aaccgcccgc cttttgtaat gtcattgaaa agacaagtga aattgggggt
     2401 ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttatg atttgcgctt
     2521 taactccgaa atatccatga tatagcggaa ttgataccga tcaagtacac ctttgatgat
     2581 ttcggccccc tctggggata actcaagaaa ttcaaaaccg atattaaggt attggggggt
     2641 atcttcatca ggccggcacc aggctacacg accagaaacg gccacacgct gcaatttctc
     2701 atccaattca tctgcaggaa attcgatgcc aatggcaaga cgcctgcctg cctcaagcga
     2761 ggtttccccg atcaccatca caccttgaag ggttaagtca ccaacatacc cgatcaattt
     2821 tttatgaagc agttcataca cgggcgtaaa agcaatcaac ttcttgcgcg attccttgcg
     2881 ttgattttgc acgacgacct cctttttata tcagaacaac ttccacaact tcacccgcac
     2941 tcaaacccgt tgcatctggg tggatgcgca aaagcccatc tgccgcggca agagtaaaga
     3001 tcaggttcga ttttccaaag atgggttcag ctttgtattt gacattcgag gctggctgct
     3061 ccgcgatcaa cttcacgggc caccaatctt cacggcccgc ctgtgaagga agattgacag
     3121 tgagcatcgc ccgcacactc gccttcggct ttggcagcat ccccaacaac ttttcgatga
     3181 caggcaccac gaacaaatat ccattcacca acgcgctgac aggattgccc ggcaggccga
     3241 tcacggcttt gccattacac acccccaaaa tcgtcggttt gccggggcgg gtgttgatcc
     3301 catgcaccag cacaccaggc tctcccagct gtcggataac ctcggcggtc atatctctgg
     3361 tggaggcgga ggaacctgcg gtgatgacga ccacgtcaca ttcggatagg gcctgcgccg
     3421 ccgcttcttt caacgctgaa aactggtcgg cgataatccc atatcttttg gttgcgccgc
     3481 cagctttttc caccaaggcc cccagcgtgt acgaattaat atcccgcacc tgcccgggcc
     3541 gcggagtttg atttggctcg atgacctcat caccagttga aatcagccca accatcacct
     3601 ttctggctac gcgcagactg gtaaatccca aagcggtcaa tccgccaatt tcggctgggc
     3661 gcaaaatcgt ccccctcgga ataacaacct gtccctgtgc cacatcctca ccaatgcgaa
     3721 tgatgttttc tccatctgct acagatttga agacttcaat ttcccctgcc ccgtcaatat
     3781 gctgggtgta ttccaacatc acaactgcat ctgcaccatt gggcaacatt ccacctgtat
     3841 ggatcaaagc acattgccct gtaccgatct caaaagacgg ggcatccccc atcggcactt
     3901 cgccaatcag gttgagatac gcgggcagag agtctgtggc gccgaaagtg tcccttgcgc
     3961 ggacggcgta cccatccacg gtggtgcgct gaaaatcagg caggggatgc ggtgcaagga
     4021 tatcttccgc agtaacacgg cccaaggcat ggagaatatc aattgaatct gaatcaaatt
     4081 cgggtgcgga aagatgcgcg atcaatttgt cacgcgcttc atcgggtggg aggagggtca
     4141 aaaattcagg catgggttat ctcatccaaa aagttagagt gaggacataa gcggtcaatc
     4201 caaaaacggc gagggcggtg gtggtgagca gcggccaatt cggtttgccg ccaagtgaaa
     4261 tgtgtcgaag caaaagcatt tgcaggatcg caaatggaag tgtaagaaag gcaggccaga
     4321 tgagcaaaaa tgaatatcca aagatcggtg aacaaaggaa aatgacatac gcaaaaatga
     4381 tcaggctgtg atgcaaaggc acagcggttt cccacccaag gcggcgaagg agtgtggcac
     4441 gttgatattt ttggtcggag ggaaaggaag tgaaattgag gatcaaaaaa taagcaagtg
     4501 ccaacgccgt gagcggaagg gcaatgataa tgagcagtcg gtgattttcc tttgcttgca
     4561 gcaaaaaccc aatggaggga gcaacatagg ccatatgcac ggcgagggca agttctccaa
     4621 atccgcgctc aagaaaacga agcggcggga cggcatacag cacgacgaga acaaaggata
     4681 aaagaagaaa gagaaaagag aaaaggttca ggctatgatt aaagtagagc aggaatgcga
     4741 tcattgcaca ggcagtgagg gcggcatagg aaatgtaaag caaattactg cgaagaactt
     4801 ccttttgcgc aggggtttcg ccttcgatca tcggttcatt gtgcgggcga aaagcctcgg
     4861 ccaacaggtt catgctcaat tgggcaagga ggatgtaacc cagcccaagt aaaaaagcca
     4921 gattctgaaa cgatttaccg agatacgcag aaatactcgc gccaagaagg tatgccagcg
     4981 cagtgaggag taaaagaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     5101 acccttccaa cctctccacc gcctgcggaa ggttcttcct gcccagcccc agtctgaaat
     5161 ggtttctgga atcatcgtac acagaccctg gcaaaagcaa aacgcccgcc tttcttacga
     5221 ggtcatcgca aaaatcttcg atatcacctt tgagcaattt cggaaaaccc attgacccag
     5281 ctttgggacg tgcccaggtg aaaagtgcag catggcggtt gaacaggtca tctacgacgg
     5341 tcaggttgtg tttgatgatc ccgagactgc gatcgaccaa aatttggcgg ttgcgcatgg
     5401 caacttccgc taggaattcg ctcggcgcgg aattacagat ggttgtgtaa tctttgagcg
     5461 aggccatggc ttgaaggatc tttttgttct tcgtagcgac ccagccaatg cgcaaccctg
     5521 caaggccata ggttttcgag gtcacaccca gcgacacggc atgttcaccc atgtcacaag
     5581 cggcggggag tctatcggcg gaatcgtatt cagattcacg gtagacctca tcggagaaaa
     5641 ggatcaggcc tttttcctgg gcgaatttgt tcaccgcttc aaaatccgca tgggacatta
     5701 agtatccagt tgggttgtgc ggcgtgttga taatgatgcc tttggtattt gtccgcatca
     5761 ggtgacggag ttcatccaga tccaaagccc agccatcctg ttcccttgct cgccacgggc
     5821 tgacgtcaca gcccacggca cgagccacct ctgcaaggga ttgatagcct ggggaatgca
     5881 caatgacatg gtcccctttt ttgaaggcgg catgcataaa aagataaatc gcttcccccg
     5941 cccctgtatg gaccaaaata tcatcgggct gcatagtggt gtatagattg gtgatctctt
     6001 tgcgcagcga tggactgccc aacgattccg tgtacccaag ccagacattt tggaacttct
     6061 cagccgagcc tggttccaag gccagcaatt ctgcaatgga catggcttca caatctgaag
     6121 agcagagtaa atattcggtg gtgaattcgt atttggcgaa atagcgttcg agtttgaagg
     6181 ggggaagtat catgattggg ctcctggtca gtggtcagtg gtcagtggtc agtggtcagt
     6241 ggtcagtggt cagtggtcag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 ngtcagtggt cagtggtcag tggtcagtgg tcagtggtca gtggtcagtg gtcagtggtc
     6421 agtaaagagt ggatgaatgg ctgagacatt accatccaca tatttgtcat ttacttcaat
     6481 gcttttttca aatcacggaa atgaagttga ttgtggatgt acagcatttt caccatttcg
     6541 cgaagaacag tttcccctaa aaatggatgg cggcccttaa tttccagttc ttcatctttc
     6601 aagccagaga cccattttac agagttggcg cgcacttctt tgtattgttc caacaactcg
     6661 gagggagata gatctttcgt ctttgcctgc tgactggcgt tgtagcggtc aatggaaaaa
     6721 tcatccgcgg cgcctgtgcc accctgacga atctgctcga aaagtttgac cagtccgcgt
     6781 tcagaggtga cgaaatgaga caacacattg cgaacggtcc acatttcacc ttcggtgtat
     6841 acctcctgct gccacagcga ttcgttcatc gcagagaaaa actcaatata tttttctcct
     6901 tcggttttca acttctcagc caattcggtt gtttcagaca ttgaaaaact ccttcgataa
     6961 ttggtaattt atggtttgtc aaattctgcg taattaatcg agcgttttcc tgtggtgaac
     7021 cagtaataat ctttgaaacc cgaaatctga ccaacgagtc ttttgctgtc ctcgaaatca
     7081 gggcggacat aaaaatagtt tgtgtcatac gggctgtcac caaaaacggc agcgaggata
     7141 tatccatttt cctcggcgta agtcttgagc aatatcgtca tgccgaacca acgctgggca
     7201 aggttggcat cggtcaactt tggcggcaca agcggcgaat aataatcatg gaacatgatg
     7261 atgtgcggtt tgtacttgtc caaatattca actgtgatct ttccgttgtg agctataaat
     7321 tggtcgttca aaccccaggt gtcgatcgcg gtccagccag aataatacgg gaccaacccc
     7381 gcctccgtgg tggcgatcac atagcctttg ccgcgatact ctgccaacaa tctgcccatc
     7441 tcatagcgcc catcgcgttc atacggtctt gcacacgtct gctggaagga tgtgaggaag
     7501 caattttgat acgacgaata atagacgagg ctcacaaaca gcgcaattcc cgcaacaaga
     7561 tacacgcgtc cattcctaac cgacagttga ttctggtctc cccctacagg gacttcgcga
     7621 tacaggctgg aaagaacacc cagccctgac tggtttcccg aaaacaaaag cggagtccac
     7681 gacatgagca cgagcggaac aagcgcatat tgaaagcgcg caccgaagtt catttcgtcg
     7741 gagatcaaga taaacgcaga cgcaaagccg agaatcggaa tgaggtatgc gagcgtccgt
     7801 gttgaggttt cgcgggagcg aaaccccagg atgaacacaa acacgaacgg caggcacatc
     7861 ctcaacgtgt tagacaggga attgtagaac gagtcccagt gcaggccgct atcgcctttt
     7921 ttgtaaaatg gatttggaag cgggtaaccg aaataattcc agcgccacac aaagtaagcc
     7981 ccacccaaca agaggaaaac actcccaaat gtgacgatga tcgcaaccga atttttccat
     8041 ccgcgcatga cgatgatcgc cagcagcatc aacgaagcaa ggatgacccc ttcggggcgg
     8101 atcaaccctg tgacaagtcc actcagggcg aaggcgagaa tcaagctgaa gggtggattc
     8161 tctttttgaa tcaggagcag gcccagggtc caggtggagg cggcggcgag ggcaaagaaa
     8221 ggcgtcccga agtaggcggc cacataggaa aatccagttc caacggcaag gtaaagtccg
     8281 cttaataatg aaaagggaat atctgtgtga ttaacgcggc gattcgtcca gtaaacgatg
     8341 aggacagtga gcaaatgcga aacaaaacca atgccccgca cagactgacc gacggtaaaa
     8401 cccaatttga tcaatgcagc agaggcggcc atgaaaagaa aatcggttgc gccatctacg
     8461 ggcgcttcgc cgatattcca gacaatgccg tgaccttctg caagatgctg ggcgtaacgc
     8521 atgagcatgg cggcatcttc aaaaggtgga atggaaaaat ccacaaagcg tgcagcataa
     8581 aacagtgtga aagcaatcag gaagatcgaa gcaagaagat cgaggggaag ttttttgaaa
     8641 gacattggca atcagtgaat agttatcagt gagcagttat cagtttttta tatttgttta
     8701 ccatttatac actacagcaa cgaatacccg ccatccacaa cgatggtttg acctgtgatg
     8761 tattcatttt tgatgaggaa ttcaagcgcg gaggtgactt cttcgagcgt ggcggcgcgc
     8821 ttgagcggaa gtttttgaac gaggttatcc cattgctctt cggtgaccac atccgatggc
     8881 aggacgagac caggcgcaat ggcgttgact cggattgtcg gcgcaagggc gcgagcaagc
     8941 agcttcgtaa gcgactcgag cccagccttg ctgaccgtgt atgatgggta gcggctccac
     9001 gccttttggg cgccaatgtc tgtaatgttg acgatcagcc cgccatcggt catccgcttt
     9061 gccgcttcct gcgccagcat aaatggcgcc cgcaggttga ggtcgagcgc caaatcccaa
     9121 tcatggatct cgagttcctg tggttttcca acgggcatga ccgccgccga attgaccagc
     9181 accttcagtg gggttttgaa ttcatccacc agtgaaaaaa ggaaatcgat tttttcaggc
     9241 agggtcaaat ctgcacggat cggaaggcaa tccacaccca gggcgcgaat ctccttgacc
     9301 gtgtggtcgg cttcattggc agaggcacgg taatgcagcg cgatggaata tcccatgcgg
     9361 gccagggata aggcaaaggc tttgccgagt ctgtgggcac cgccagtgac aagtgcgaga
     9421 ggcatacagg gattgtaaac gaaaaacgca gagcaggtaa aatgttattg ttacactcag
     9481 cagccgatcg ccaaaaggaa ccaacatgga aggctcaacg tcatctttgc atgaaaaagc
     9541 accccgctgg agctggatga acaaccccat tatcgccaag gaactaaaag gccgcatgcg
     9601 cggcaggcag ggatttgtga tgctgaccgc ctacctcggg ctgatcagcc tcgtcattta
     9661 cgccgtctat gtctattcag tacgcaacac ctacagccgc agtcctggct ttttacagga
     9721 ggcaggcaaa caggtctttt tgatcgttgt gcttctggaa atcatcctga cgggattcat
     9781 cggccctgcg ctcacgtctg gtgcaatttc gtcagaacga gagcggcaga cctacgacca
     9841 gctgcgaatg tcactgctca gcgcaaaaga cattgtgatc ggcaaactgg ggtcatccgt
     9901 catgtacctg ctcttgctca ttgtcgctgc aattcccctg caaagccttg ctttctttct
     9961 gggaggcgtg ggcattgagg aggttctagt ttccatctgg attctcaccc tttccacgat
    10021 caacttctgc acactgggct tgtacttttc aggcatctcc aaacggacat ccatcgcaac
    10081 catcctgtcc tacaccaccc tcatcctgcc gatcctctta ttcgcagcgg ggatctatgc
    10141 cataaatgaa aattcctacc tgctctcaaa tgccagcttc gagtttcaac gctggctgct
    10201 caacgtgttg tggaccatca tttcaaccaa cccagcttct gccgccttcg tcagcgaagc
    10261 catgctgatc gaagaacaaa atttattcct gatccaatca cccgtaccgc cgatggtcct
    10321 ggtgtcacca tggattttgt actcgatctt ctctataaca atgaccattt taatgataag
    10381 gttaatcgtc ttttacctga agcgtcacga gccttaatgg agcaaacgac catgagcacg
    10441 atcaatcatc tcaatcacac cctccaaaac tggattcaat ggttacgcgt ccagcgcgca
    10501 ctgacctggt atctgcgcgg gttggccgca gcgctggggc tctccctgct ctttggcggc
    10561 atcgggctgg ctcaggcaaa actcttgaaa caggaatttc tcgcgcttgt cgttgcctcg
    10621 gtcactttat tgccgatcct ctcagcgatc atcgcatttt tctggaaagt aaatacaaca
    10681 aaagccgcgc gtcattttga ccgcgccttt catctcgaag aacgcatcag cactgcgctg
    10741 gaactaagca ccgcggacca cgccaacgaa atggttcaaa agcaattgga cgacgcggtc
    10801 tccgccgcgc accgcatcca accccgccga gacctgcctc tgcgactaaa aagattcgac
    10861 tcagccctga ccgtgatcct cgcgctgttg atcggtgttt tatggttccg cggtgatgcg
    10921 ctgttcgccg ctgcaagccg tcagcgcgct gtggagcagg ccatcgccgc acaacaggaa
    10981 cagatcgaag agatcatcaa agagatcaac gaaaacgaag cgctgaccgc cgaacaaaaa
    11041 caggaactga ccgaaccgct caaccaggcc ttgcaagccc tcgaccagaa cccatcgctc
    11101 gaaggtgctg tttccatttt gacgaataca ggcgaacaac tgcaagccct gaacagcgag
    11161 caggcaggtc aaaccgcaca ggcgttgaaa gaggcgggca gttccctgca agcacaagaa
    11221 gggactccgc tttcatcggt ggggcaaaac cttgcaagtg gaaatttcac acaagccgcg
    11281 aatgacctca acaacatgga cttgagtcag atgagccctg aagagttgca aaaactcgcc
    11341 gaccaactgg aagccacggc acaatcactg gcatccacaa acccgcaatt ggcacaggcc
    11401 atgcaacaag ccgcagacgc gatccgcaat ggggatttgg ccgcggcaca gcaggcgctt
    11461 gccaatgcct cgcaacaaat ggcgaatgca ggtcagcaag tcactgccgc gcagactgcg
    11521 agtcaggcgg cgggccaaat gcaacagggt gcaggtcagc tattggcggc gggcggcggg
    11581 aatcaatcgg cgcaggcaaa tggtcagggt caggggcagc agggaaataa ttcgggtgga
    11641 caaaatagca gcgctggatc aggctcgggg tcgggtgatt cgccagattc tcctcaacag
    11701 ggaaatgagg cgggcagttc tcccatcgag caaaataacg gcgcaggcga tggcggcgaa
    11761 tccacatacg aacagatcta tgctccatcg ttgcttggcg gcgaaaacgg agagaacgtt
    11821 gggcttccca cttctggaaa tgacggcgat gtggtcggca ccagccccac caccgcgacg
    11881 gatggtcaaa gccttgtgcc gtacaccaat gtgtttacgc aatacgagca gttctattat
    11941 caagccatcg aaaatggaca agtgcccatg caattctttg acttgatccg aaattatttt
    12001 atctctttga aataacggag cgaccatgag cgatttttct gttgacacct tccgcgaata
    12061 tgcaaaccga atcgaaaccg aagtggggcg cgtgatcgtc ggtcagggcg atgtggtacg
    12121 gcatgtactg gtgagcatcc tcgcgggcgg acacgtcctt ttggaaggcg tgccagggtt
    12181 gggcaaaacg atgttgatcc gcacgcttgg gcaggcattg gatttgaaat tctcgcgcat
    12241 ccaattcacg ccagacctga tgcccgctga cattctcgga acagaaatcc tcagcgacga
    12301 caacggcaag cgcgaatttc gtttccgcga agggccgctg ttctcaaatt tggttctggc
    12361 agatgaaatc aaccgcgcca caccgaagac gcaatctgcg ctgctcgaag cgatgcagga
    12421 ggcctcggtg acggtggcgc aaagaactta caaactcgaa gaaccgttct tcgtgatggc
    12481 gacacaaaat ccgctcgaaa tggaagggac ctatcccctg cccgaagcgc aactggatcg
    12541 tttcttcttc aaagtgaatg tggatttgcc caacgtgggt gaaatggtcg agattttaga
    12601 ccgcaccaca ggcaaggaat cacctgcggc aagcaaagcc gtcactgggg ccgatatcat
    12661 ccaaatgcgc gcgctcgccc gcac
//
LOCUS       DAOQLC010000122        12349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_1961_length_12349_cov_17.820970, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000122 DAOQLC010000000
VERSION     DAOQLC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12349
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1961_length_12349_cov_17.820970"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1466
                     /locus_tag="PL000_17210"
     CDS             273..1466
                     /locus_tag="PL000_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC90671.1"
                     /translation="MFAPTVFALTEVRGKNLAANTIGMVLRSVMTFHLYLDARGIDFE
                     GRMASGDLLSLGEVEDLARLCRRPLAELAALSRPSEGSDANVVSLEKVRMGVPKALTA
                     DLDPNVVASRLRYIRMYIQWLATERLSRHGLAQSVASRLGDSTRFVADAIDTRIPHGS
                     GRNTLGQREGVADEVVAELLRVVDPHSPNNPWRDQHTRYRNALLIHWLLYLGLRIGEA
                     LGVRVSDIVAYRKEVTIHRRADDPDDPRRYQPQTKTRARVLPVSGTLLAETQAYILNH
                     RSALPQAKKHAFLFVASRTGQPMTLAAVGKLFKELRERCPLLPEGLSGHVLRHTWNDA
                     FSKKMDEAGVTPENERQARSYLMGWSPTSDTAAVYTRRYVRNKAKEVSLGLQEQLVNK
                     GKGNE"
     gene            1459..3003
                     /locus_tag="PL000_17215"
     CDS             1459..3003
                     /locus_tag="PL000_17215"
                     /inference="COORDINATES: protein motif:HMM:NF041502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNC90672.1"
                     /translation="MSKLVEVAAHRGAAPELPELARTRSGVLFDPHADRWAYRDATAT
                     VNLDFATWPVTETFVASAKLTLLWYAEQMSPSHLKNMHERLMHFLKTACARRDGFLQE
                     VTSTDLINYRATLTDATSWYLGSLSGLLQKWNSLGYPGVTAEAIALLRQLRTQGNRKG
                     EAVLTHDPIHGPFTDMELESLHASLDKAYADGEVDREGYLLAYLYMLLGQRTVQYAAL
                     KVRDVGVAYAKDGTPLYTLRVPRAKQRNQISRAEFRDRVLIPQIGEPLVLHSHEVQKS
                     FHGLLPEPLDSPLFPARRRIENEPKGFEHHRTAQALGQELKKILGGLRAMSERTGQPL
                     HITPTRFRRTVATRAAMEGHGELIIAELLDHSDTQNVGVYIEARPEIVERIDRAVAMY
                     LAPMAQAFAGVIIDDESQAKRAGDPSSRVCDPRFSPSMKPMGNCGKHGFCGFLAPIAC
                     YTCVNFQPWLDGPHEAVLDYLIGERDRLAAQTDLRIASVNDRTILAVAEVVRLCEARR
                     SGVADG"
     gene            2996..5044
                     /locus_tag="PL000_17220"
     CDS             2996..5044
                     /locus_tag="PL000_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90673.1"
                     /translation="MAEIVLFTPKAELDAAANLRGFVDMCRSKLSVFGADLPFQNDVW
                     DVTEAVATKGKGGKRERITFSNAATVDQKSPEMMREPFLSFAKAYVRYMHGMRPTKII
                     HNRVAALRAIEAALLETGLPPDPVQIDSRILNRAAQIVADRFSDGAAYRVGGQIEMLG
                     IFLSENRLAAVPARWRNPIKRPSSAVRVGKEFDERREEKMPSSAALDALPRIFRIATE
                     PADVITVSVAAILLASPDRISEVLTLPEACEVREPRKGKEDAYGLRWWPAKGAEPMVK
                     WLVPSMASVVEEALQKIRTATAEARRIAEWYEQHPGQLYLAADVVHLRSREWLSLEDI
                     AKVLGLSDSSAANTWCRAKGIKPEQREGEGKRVFARFSDVEAAVLKMLPRGFPVLDQA
                     TGLKYSEALFVVQCNQLNVRIGMHRCMIEPVGIGQINTGLGSRVAHGYESIFSRFGFT
                     EPDGSPIQITSHKFRHYLNTLAQAGGLSQLDIAKWSGRRDIKQNEAYDHVTPSQMLQK
                     IRDAVGGDQMFGPLAELPKKVLIRRDEFARLVVPTAHTTDLGYCVHDYTASPCQLHMD
                     CIHCRDLVCVKGDTEKERLLRRRLDEAKGLMERAQAATSEGYSGSDRWLDHHRTTVDR
                     LAQLCSIMDDPRVPDGAVIQLAAHNMPSRIDRVTERGQFQPENEQVRLLAGVKALLGE
                     "
     gene            5716..8265
                     /locus_tag="PL000_17225"
     CDS             5716..8265
                     /locus_tag="PL000_17225"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC90674.1"
                     /translation="MAAKEAKARIKINKLLEGSGWRFFDDATGKANIVLEPKVKLTKA
                     VIDALGDNFEQSSNGFIDFLLLDDAGFPLVVLEAKSEDKNPLVGKEQARKYAKSQNCR
                     FVILSNGNLHYFWDLEQGNPHIVTRFPTPDSIKSYHRFQPNPQRLITEPVGKDYIALT
                     QMPSYAAEAGWNNPAERDAFILKNRLRFLRDYQKRAVQAIQDAVAEGSSRFLFEMATG
                     TGKTLTAAAVIKLFLRTGNARRVLFLVDRLELEDQANKAFITQLKNDYTSVIYKERRD
                     EWRKAEIVVTTVQSLLFNDKYRRLFSPTDFDLVISDEAHRSIGGNARAVFEYFVGYKL
                     GLTATPRDYLKKYDASKPSTRDPRETERRLLLDTYRTFGCESGQPTFRYSLLDGVRDG
                     FLINPVVVDARTDITTQLLSDEGYAVANTNENGEAEQESFFQKDFEKKFFSEATNRLF
                     GQTFLNNGLRDPISHEFGKSIVFAVSQNHAAKLTQILNEQADALWPGHYQSDFAVQVT
                     SQIPDAQQYTINFTNNNLLGSANFLDAYKTSKARVCVTVGMMTTGYDCPDLLNLALMR
                     PVFSPSDFVQIKGRGTRKHGFLEQLFDKQLKEQIGKQEKTQFKFFDFFANCEYFEEKF
                     NYDEVLKLPRPGAGEGNGDGGGTTISPTGEYIHGGSDNIQHVKEEAVGPQGMKIDRMF
                     FEAFEKTVKADDALKSFVDNEQWDNAVEYVAANVLNRPAEFFTLDKLRRAAGVDRRLT
                     LREILEKIFGLIPGFKSKDELLEDEFQKFLLDQPEDELAKHADTIVALKYFFKAYATD
                     GRLRDIIEKRRLTELNVNPAFSMADFKAVPKEWRDRLPEYVKDYVSLNQFM"
     gene            8285..10147
                     /locus_tag="PL000_17230"
     CDS             8285..10147
                     /locus_tag="PL000_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC90675.1"
                     /translation="MLDADTKRRIDTCRDILVGKVPDPKSQVEQITIALIYKFMDDMD
                     AEAEELGGDRTFFAGNYAKYGWAKLMASYMGGFEVLALYSEAIQKMNENPGIPALFRD
                     IFKNAYLPYRDPETLRSFLKEIDGFRYDHSERLGDAFEYLLSVLGSQGDAGQFRTPRH
                     IIDFMVEVIDPKKNESVLDPACGTAGFLISAWKHILKQNTKQRAGDQLTPDERTNLAA
                     SIHGYDISPDMVRLSLVNMYLHGFTDPHIVEYDTLTSEERWNETVDVILANPPFMSPK
                     GGIKPHKRFQIQSKKSEALFVDYIIEHLAPSGRAAIVVPDGIVANPSTGYVQLRRLAL
                     NNGLFAVVSLHPFVFKPYAGAKTSILFFDRKRIREQKTDVLFLRVDNDGFERGEQRRR
                     IDLDDLPMAKGIIQSFRDGLKLNSESDSIAYAVVDTNQITGTLSLFANKYLHQGQLNA
                     DLVKIADLFNIEKGSLQSTKAEDGEFNFITAAEEWSTNKTYTHDCEALVFAHGASGSL
                     GRTHYVNGKFIASDLCFILTPKIGGKAVDLRFYHAYFSVIREEIVAALAKGAAKLSIN
                     KSDFGSHAIAYVPIETQQELGNLIQQEQNRIRDLKRQIVEAEQNVEASVYEALG"
     gene            10220..11827
                     /locus_tag="PL000_17235"
     CDS             10220..11827
                     /locus_tag="PL000_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HNC90676.1"
                     /translation="MTSSLATILGRGYFPKELPPPFNTKLYGAFAETAPAVFHLDTSK
                     NGSKGNLVSRPAIHNLARTGTLRRKLTIPNPVNQYQVARAVAEGWAELKVVCKQSPIS
                     LTTPRYLKHGARAINPRSPFDAIPLARARSRTASRYLLATDLSQFYPSIYTHSIPWAL
                     HTKAVAKSKPNDYSLLGNVLDLAMRNGQDKQTIGIPIGPDTSLVIAEAILSAVDAQLS
                     GAMTKRGFRYIDDIACGFRTVAEAEETLGRLQHLVGDLQLQLNPRKTRIVELPSELEA
                     SWVPHLRLFAFRSSTPGAQQRDLLSYFGWAFELAANQREEAILRYSVQRMRSVSIFEK
                     NWGLYESLLLQCLSVEPGTAPAVIGELYKYHLRMPLDRARITESLQQLIEDHAPLHHG
                     SEVVWALWGAICLGCTLDAKVVEKSLLASDPCVALCALHIRAKGLVTGPVDVSPLEAL
                     MHDGELTDTQWLLAYEANVKGWLPNKAGKDFVAAHKHFGPMKAAGVSFYDESAMLPIV
                     VKAAVVQGYGDADDDFDLAYLVGNVSG"
BASE COUNT         2836 a         3276 c         3492 g         2745 t
ORIGIN      
        1 gcgccagcca ggaagacgcc cagctttgac gacatggacg acgacatccc gttctagatc
       61 cgacctgggt tgtagctgta aacaagatcc cggtaagcct tgtgcttact gggatttttt
      121 attgtttact cttttttctg tttgtctcta tactgttcgc tatcgtatac ctttaaaaat
      181 tgtaaacaat gtggtcaaac taccgcgtta agactgttgt gctttcctcg ggggagcgtc
      241 ttcctgttct tcttgatcgg gatgggcagc caatgtttgc tcctaccgtc tttgcattga
      301 cagaggttag aggaaagaac ctagcagcca acaccatagg catggtcctt cgcagtgtga
      361 tgacttttca tctgtatctt gatgcacgcg ggatcgactt tgaggggcgt atggctagcg
      421 gtgatttgct ttctcttggc gaggtggaag accttgctcg cctctgtcgg cggcctcttg
      481 ccgagttggc tgcgctgtcc cggccaagtg aaggtagtga tgcgaatgtg gtatctcttg
      541 agaaggtcag gatgggggtt ccgaaggcct taaccgcaga ccttgatccc aatgttgtgg
      601 caagccggct gcgctacatt aggatgtaca ttcaatggct cgcgaccgag cgtctgagca
      661 ggcatggttt ggcacaatca gtggcgtccc ggcttggcga ttcgacaagg ttcgtagcag
      721 atgcaatcga tacgcgcatt ccgcatggat cgggtcgtaa cacgctcggc caacgagaag
      781 gggtcgcgga tgaagtcgtg gcggagctat tgcgcgttgt tgatccgcac tctccgaaca
      841 atccgtggcg ggatcagcac acacgatatc gcaatgccct ccttatccac tggctcttgt
      901 atctcgggct gcgcattggt gaggcgctgg gtgtgcgtgt aagtgatatc gtggcctatc
      961 gcaaagaagt cacgattcac cgacgcgccg atgatcccga tgatcctcgg cgataccagc
     1021 cgcaaaccaa gacccgggca cgggtcctcc ctgtcagcgg cacgctgctg gcagagacgc
     1081 aagcctacat cctgaaccac cgaagcgcat tgccacaagc aaagaagcat gcatttctgt
     1141 tcgtcgctag tcgaacaggt cagcccatga ccttggccgc tgtggggaag ttgttcaagg
     1201 aactgcggga acgctgcccg ttgttgccgg aaggactctc tgggcacgtt ttgcgccaca
     1261 cctggaacga cgcgttctcg aaaaagatgg atgaggccgg tgtgacccct gaaaacgagc
     1321 gccaggctcg tagctaccta atgggctggt caccaacatc ggatacggct gctgtctata
     1381 cccgccgcta tgtacgcaat aaggcaaaag aggtttcact ggggctgcaa gagcaacttg
     1441 tgaataaggg gaagggtaat gagtaagctg gtggaagtcg ccgcgcatag gggggctgct
     1501 cccgaattac ctgagttggc gcggacacgg tcaggtgtcc tgtttgatcc gcatgcggac
     1561 agatgggcat accgtgatgc aacagctacc gtaaatctag actttgctac gtggccggta
     1621 acggaaactt tcgttgcgtc agcgaagctt accttgctct ggtatgcgga gcagatgtcg
     1681 ccgtcacatc tgaagaatat gcatgaacgt ctgatgcatt ttctgaaaac cgcatgcgcc
     1741 aggcgggatg gctttttaca ggaagtgacc agtaccgacc taattaatta cagagcaacc
     1801 ctgaccgatg ctacgtcatg gtatctcggg agtctttccg ggctattgca gaaatggaat
     1861 agcctcggat atccgggagt gacggctgaa gcgattgccc tattgaggca actgcgcacg
     1921 cagggcaacc gcaaggggga ggcggtgcta acgcatgacc cgatccatgg cccgttcact
     1981 gacatggagt tggaatctct gcatgcgtcg cttgataagg cgtatgcgga cggcgaggtg
     2041 gatagagagg ggtatttgct cgcttatctt tacatgttgc tcgggcagcg tactgtgcag
     2101 tatgcagcat tgaaggtacg tgacgttggc gtggcatacg cgaaagacgg tactcctctg
     2161 tatacgcttc gcgtcccccg cgctaagcag cgtaatcaga tttcacgcgc tgagttcaga
     2221 gatcgcgtgc ttattcccca gatcggtgaa cctctggtac ttcattctca cgaggttcag
     2281 aaatcgtttc atggactcct gccggaaccg ttggattcac cattattccc cgcgagacgg
     2341 cgaattgaaa atgagcccaa agggttcgag caccaccgta ccgcacaggc tcttgggcag
     2401 gagctgaaga agattcttgg cgggctgaga gccatgtcgg aacgaacagg gcagccattg
     2461 cacatcaccc ccacacgctt ccgccgcacc gtggccacaa gggctgctat ggaaggtcat
     2521 ggcgagctga tcattgccga attgttggac cacagcgata cccagaatgt tggggtatac
     2581 atcgaggctc gacccgaaat cgtggagagg atcgaccggg cggttgccat gtatctggcg
     2641 ccgatggcac aggctttcgc cggagttatc atcgacgacg aatcccaagc gaagcgcgca
     2701 ggagatccgt ctagcagggt gtgcgacccg agattttccc cctctatgaa gcccatgggt
     2761 aactgcggca aacatggctt ctgcggcttc ttggcaccca tcgcctgcta cacctgcgtg
     2821 aatttccagc cgtggctgga tgggccccac gaggcagttc tcgactactt gatcggcgaa
     2881 cgggatcgac ttgcggctca aaccgatcta cgtattgctt ccgtaaatga ccgcaccatc
     2941 ttggctgtcg ccgaagtggt caggctgtgt gaggctcggc gcagtggagt ggctgatggc
     3001 tgaaatagtc ttgtttacgc ccaaggcaga acttgatgct gcggcgaatc tacgtggttt
     3061 tgttgatatg tgccgtagca agcttagcgt cttcggggcg gatttgccgt tccaaaacga
     3121 cgtgtgggat gttacggagg cggtcgctac caagggtaag ggcggcaagc gagagcgcat
     3181 cacgttctcc aatgcggcta cggtcgatca gaaatcgccg gagatgatgc gtgaaccatt
     3241 cctgtccttt gcgaaggcat acgttcgtta tatgcatggg atgcgaccaa ccaaaataat
     3301 tcacaacagg gtggctgcat tgcgggccat cgaagcagcg ttgttagaga caggtttgcc
     3361 tcctgacccg gtgcagatcg attcgcggat cctaaaccgt gcggcgcaga tcgtcgccga
     3421 taggttcagc gatggagccg cataccgtgt aggtggtcag atcgagatgt tggggatttt
     3481 cttgtcagaa aatcggcttg ccgctgtacc tgcgcgttgg cgaaacccga tcaagcgtcc
     3541 cagcagtgcc gtgcgcgtcg gcaaggagtt cgatgaaaga cgtgaggaaa agatgccgtc
     3601 ctcggctgcg ctcgatgctt tgccccggat tttccggatc gctaccgagc ctgctgacgt
     3661 aatcaccgtg tcggttgcgg cgatccttct tgcctcaccg gatcgtatca gtgaagtcct
     3721 cactcttcct gaagcctgcg aggttcgcga gcccagaaag ggcaaagagg acgcatacgg
     3781 cttgcgctgg tggccggcca agggggccga gccgatggtc aagtggctgg tgccctccat
     3841 ggcatctgtc gtggaggaag cacttcagaa aatccggaca gccacagcag aggctcgacg
     3901 aattgcggaa tggtatgagc agcatcccgg tcagctttat ctggctgcag atgtggtcca
     3961 cctgcggagc cgcgaatggc tgtcgctcga agatattgca aaggttctcg gactttccga
     4021 tagcagtgct gcaaacactt ggtgtagggc gaagggtatc aagccagagc agcgcgaagg
     4081 ggaggggaag cgtgtctttg cacggttctc agacgttgag gccgccgtgc taaagatgtt
     4141 gccgcgcggg tttccagtct tggaccaagc aacaggactg aaatatagcg aggccctgtt
     4201 cgtcgtgcag tgcaatcagc taaatgtgcg gattgggatg catcggtgca tgatcgagcc
     4261 ggtcggtatc ggccagatca acacggggct aggcagccgt gtggcgcacg ggtatgagtc
     4321 gatcttcagc cgcttcggct tcaccgagcc agatggcagc ccgatccaaa ttactagcca
     4381 taaattccga cactacctta acacgcttgc gcaggccggt ggtctgagcc agttagacat
     4441 tgccaagtgg tcagggcgcc gggatatcaa gcagaacgaa gcgtacgacc acgtcacccc
     4501 cagccagatg cttcaaaaaa tccgggatgc tgttggtggc gatcagatgt tcgggccact
     4561 agcagaacta ccgaagaagg tgctgatccg ccgggacgag ttcgctaggc tggtcgtgcc
     4621 gaccgcgcat accaccgacc ttggctactg cgtacatgac tacacagcat caccctgcca
     4681 attgcacatg gattgcatac attgtcggga cttggtgtgc gtgaagggcg atacagagaa
     4741 ggaaaggtta ctgagacgcc gcctggatga agctaagggg ttgatggaaa gggcgcaagc
     4801 ggcgacatcg gagggctact ctggcagtga tagatggctt gatcaccatc ggacgacagt
     4861 ggatcgactg gctcaactgt gctcgattat ggatgacccg agagtaccgg acggtgcagt
     4921 catccagttg gcagcccaca atatgccgtc acgaattgat cgggtaaccg agagaggtca
     4981 gttccaaccg gaaaatgagc aggtccgtct actcgccggt gtcaaagcct tgttggggga
     5041 ataatcttga acacactgag aaagaaacct gtggtccgca ggggtaagaa cctgaccgac
     5101 gatgcaattg agcagatcgt ccggctccta gatggttggg aaggaaaatt aacctgggag
     5161 acgttgatcg ccgctatcgt gactaggctg cattgcgagt acacaaggca ggcattgcat
     5221 aagcatgaac gtattcgtgc agcctataca ctgcgtaagg agtcgctcgc tgggcatagg
     5281 gatgcggctg tttcgagagg atcagggccg cttgcggatg caatggcacg tatagcccgt
     5341 ctcgaagcag aaaaccaacg cttggaggct gagaaccaga ggttgcttga gcagtttgtc
     5401 gtgtgggctt acaacgcgca tacacgtggg ctggacaagg agtttctgag tcaaccactg
     5461 ccacgcgtaa atagggacca gacggcacca ctgaaattag cgaagaaagc gacgggaaag
     5521 cgatagccgg gcgcttttca gcgcattagc tttggcagcc cggcgttagt gcgggtagcg
     5581 ttttcggttg ctgccgggtg agtgatttct acatctcggc caagtaaaaa cctatttgga
     5641 attatggttc ttccgtgcgt tgctttacac tctcccctca taaatacatt catctgcgac
     5701 gcagggagcg ccacagtggc agccaaagaa gcaaaagcac gcatcaaaat caacaaactg
     5761 ctggaaggtt ccggctggcg attctttgat gacgccacag gcaaggcgaa catcgttctc
     5821 gaacccaagg tcaagctgac caaggcagtc atagacgcgc tcggcgataa cttcgagcaa
     5881 tccagcaacg gcttcattga ttttttactc ctcgacgacg ccggcttccc gctcgtcgtc
     5941 ctcgaagcca agtcggaaga caagaatccg ctggttggca aggagcaggc gcgcaagtac
     6001 gccaaatcgc aaaattgccg tttcgtcatt ctctccaacg gcaacctgca ctacttctgg
     6061 gatctggagc agggcaatcc gcacatcgtt acccgcttcc cgacgccgga ttcgattaag
     6121 agttatcacc gcttccagcc gaatcctcag cgcctgatta ccgagccggt cggcaaggac
     6181 tacattgcac tgacccagat gcccagctac gccgccgagg ccggctggaa taatccggca
     6241 gagcgtgacg ccttcatcct gaagaaccgc ctgcgcttcc tgcgcgacta ccagaagcgt
     6301 gccgtgcagg cgattcagga tgccgttgcc gaaggcagta gccgcttcct gttcgagatg
     6361 gccaccggca ccggcaagac gcttaccgcg gcagccgtca tcaagctctt cctacgcacc
     6421 ggcaacgccc ggcgcgtgct cttcctcgtg gatcggctgg agctcgaaga ccaggccaac
     6481 aaagccttca ttacgcaact caagaacgat tacacctcgg tgatttacaa ggagcgccgc
     6541 gacgagtggc gcaaggccga gatcgttgtt actacagtgc agtcgctgct attcaacgac
     6601 aagtatcgtc ggcttttttc gccgaccgat ttcgatctgg tgatttcgga cgaagctcac
     6661 cgctccatcg gtggcaatgc ccgcgccgtg tttgagtatt tcgtcggtta caagttggga
     6721 ctgactgcca cgccacgcga ttaccttaag aagtacgacg ccagcaaacc gagcacgcgc
     6781 gacccgcgcg agaccgagcg tcgcctgctg ctagacacct accgcacctt cggttgtgaa
     6841 tcggggcagc cgaccttccg ctattcatta ctcgatggtg tgcgcgacgg ctttctcatc
     6901 aatccggtgg tcgtcgatgc acgaactgac atcacgacgc aattgttgtc tgacgaaggc
     6961 tatgctgtcg ccaataccaa cgaaaatggc gaagcggaac aagagagctt tttccagaaa
     7021 gatttcgaga agaagttttt ctccgaggcg accaaccggc tattcggcca gactttcctc
     7081 aataacggtc tgcgcgatcc gatcagtcac gaattcggca agtccattgt gttcgccgtt
     7141 agccagaacc acgctgccaa gctgacgcag attctcaacg agcaggccga cgcgctttgg
     7201 ccgggccact atcagtccga ttttgctgtg caggtaactt cgcaaattcc cgacgcgcag
     7261 caatacacca tcaacttcac caacaacaat ctgctgggtt cggccaattt tctggacgct
     7321 tacaaaacca gcaaggcgcg ggtttgcgtc acagtcggca tgatgactac cggctacgac
     7381 tgcccggacc ttctcaacct ggcgctaatg cgcccggtgt tctcgccatc cgacttcgtg
     7441 cagatcaagg gccgaggtac gcgcaaacat ggcttcctcg aacagctttt cgacaagcag
     7501 ctcaaggagc agatcggcaa gcaggagaaa acgcaattca agtttttcga ctttttcgcc
     7561 aactgtgaat acttcgaaga gaaattcaac tacgacgagg tgctgaagct gccccggccc
     7621 ggtgcaggcg agggaaatgg cgacggtggc ggcacgacca tcagcccgac cggcgaatac
     7681 attcacggcg gctcggacaa catccaacac gtcaaggaag aagccgtcgg cccgcagggc
     7741 atgaagatcg accgcatgtt cttcgaagcc ttcgagaaga ctgtgaaggc cgacgacgca
     7801 ctgaagtcct tcgttgataa cgaacagtgg gacaacgcgg ttgaatatgt tgcggctaat
     7861 gtgctcaaca ggccggctga atttttcacc ctcgacaagc tgcgtcgcgc tgccggcgtg
     7921 gatcgccgcc taactttgcg cgagattctg gaaaagattt tcggtctgat tcccggcttc
     7981 aaatcgaaag atgaactgct cgaagacgaa tttcagaagt ttttgcttga ccaacccgaa
     8041 gatgagttgg ctaagcacgc tgacaccatc gtcgcactca agtacttctt caaagcgtat
     8101 gcgaccgatg gccgcctgcg cgacatcatc gaaaagcgcc ggctgaccga actcaacgtc
     8161 aatcccgctt tcagcatggc cgacttcaaa gccgtgccca aggagtggcg ggatcgcctg
     8221 cctgagtacg tgaaggacta cgtatctctc aaccaattca tgtaatagaa agaaacatcg
     8281 aataatgctg gatgccgaca ccaaacgccg aatcgacacc tgccgcgaca tccttgtcgg
     8341 caaggtcccc gacccaaaat cacaggtcga acagatcacc atcgcgctga tctacaagtt
     8401 catggacgac atggacgccg aagctgaaga actaggtggc gaccgtactt tcttcgctgg
     8461 caactatgcg aaatatggct gggccaaact catggcctcc tatatgggcg gcttcgaagt
     8521 gctggcactg tattccgaag ccatccagaa aatgaacgag aaccccggca ttccggcgct
     8581 gttccgcgac atcttcaaga acgcctatct gccctaccgc gacccagaaa ctctgcgcag
     8641 cttcctcaag gaaatcgacg gtttccgcta cgaccattcc gagcggctcg gcgatgcatt
     8701 cgaatatttg ctttcggtgc ttggcagcca aggcgacgcc ggccagttcc gcacgccgcg
     8761 ccacatcatc gacttcatgg tcgaggttat cgacccgaag aaaaacgaaa gcgtgctcga
     8821 ccccgcctgc ggcacagcgg gcttcctgat ttcggcgtgg aaacacatcc tcaagcagaa
     8881 cactaagcag cgagcgggcg accagctcac tcctgatgag cgcaccaatc tggcggccag
     8941 catccacggc tacgacatct cgcccgatat ggtgcgcttg tcactggtca atatgtacct
     9001 ccatggcttc accgatccgc acatcgttga gtacgacacg ctaactagcg aggaacgctg
     9061 gaacgaaacc gtcgatgtta ttttggctaa tccgcccttc atgtcgccga agggcggtat
     9121 caagccgcac aagcggtttc agatccaatc caagaagtct gaggcgctct ttgtcgacta
     9181 cataatcgaa catttggctc cttcaggtcg tgctgcgatt gttgttcctg atgggattgt
     9241 cgcgaaccca tcaaccggct atgttcagct caggcgtctc gcacttaata atgggctgtt
     9301 tgccgtcgtg tcgttacatc cttttgtatt taaaccttat gcaggtgcaa aaacgagcat
     9361 tcttttcttt gacagaaaga ggattagaga gcagaagacg gacgttctat ttctgcgtgt
     9421 agataacgat ggtttcgaga gaggggaaca gcggcgccga atcgatctcg atgatttgcc
     9481 catggctaaa ggaataattc agtcatttcg cgatggtttg aaattgaatt ccgaatcaga
     9541 ttccatcgct tatgcagtag tggatacaaa tcagataacc ggcactttga gcttattcgc
     9601 caataaatac ttacatcaag gccaattgaa tgcagacttg gtgaaaattg ccgatttgtt
     9661 caacattgaa aaagggtctc tacaaagcac gaaggccgaa gacggcgagt tcaatttcat
     9721 tacagcagca gaggagtgga gtactaacaa aacgtacaca cacgactgtg aagcattggt
     9781 tttcgctcat ggcgcttcag gttcgcttgg gcgaacccac tatgtgaatg gcaagttcat
     9841 tgcgagcgat ctttgcttca ttcttacgcc aaagattggg gggaaagctg ttgatctacg
     9901 gttctaccat gcctacttct cggtaattcg cgaggaaatc gtagcagcgt tggcgaaagg
     9961 cgcggccaaa ttgtctatca ataaatccga ctttggcagt cacgcaatcg cttacgtgcc
    10021 gatcgagact caacaagagt tgggtaactt aatccaacaa gagcagaaca gaatccgcga
    10081 tttaaagcgc caaattgttg aagcagaaca aaatgttgag gccagtgttt atgaggcgtt
    10141 agggtgattt gccagtgcca ccaaaaaagg acatggggct cttagcgaag tcggtgcagg
    10201 catacatgat ggatgtggta tgacttcgtc gcttgctacc attctcgggc gagggtattt
    10261 cccgaaggag ctgccgccac cattcaacac gaagctatac ggtgcctttg cagaaacagc
    10321 cccggcggtt tttcacttgg acacatccaa gaatgggagt aaggggaatc tggtgtcgcg
    10381 gcctgccatc cacaatctgg ctcgcaccgg gacattgcgg cgcaagctga caatccccaa
    10441 tccggtcaat cagtatcagg ttgcgcgcgc ggttgccgag ggctgggcgg agttgaaggt
    10501 ggtatgcaag cagtcgccga tttcgctgac aacgccgcga tacctgaagc atggagctcg
    10561 tgcgatcaac cctcgcagcc ccttcgatgc cattccgctt gctcgggcac gctcgcgcac
    10621 ggcatcacgt tacctgctgg cgactgacct cagccagttc tatccgagca tctacaccca
    10681 ttcgattccg tgggcactgc acaccaaggc cgttgcgaag tcgaaaccga atgactactc
    10741 gttgcttggc aacgtgctcg acctggcaat gcggaacggc caggacaagc agacgatcgg
    10801 catccccatc gggccggata cgtcgctcgt tatcgccgaa gcgatccttt cggcggttga
    10861 tgctcaactt tccggcgcga tgacgaaacg tggctttcgt tacatcgacg atatcgcctg
    10921 cggatttcga accgtcgccg aagctgagga aacgctgggg cggttgcagc acttggtggg
    10981 tgatcttcag cttcaactga atcccaggaa aacgcgaatc gttgagttgc cgtcagaact
    11041 ggaggcgtca tgggtgccac atctgcgttt gttcgcgttt cgttcatcaa ctcccggcgc
    11101 gcaacagagg gatttgttga gttacttcgg ctgggctttc gagctggcgg caaaccaacg
    11161 cgaagaagcc attcttcgct attcggttca gcggatgcgt tcggtctcca ttttcgaaaa
    11221 gaactggggg ctttacgaaa gcctgctcct gcaatgcctt tccgttgaac ccggtactgc
    11281 gccggcggta attggcgaac tttacaagta ccacctgcgc atgccacttg atcgtgctcg
    11341 cattaccgaa tccttgcagc agttgatcga ggatcacgca ccgctgcatc acggtagcga
    11401 agtcgtttgg gctttgtggg gcgcaatctg cctcggctgc acgcttgatg caaaggtggt
    11461 tgagaagtct ttgctggcat ctgacccttg tgtggcgctc tgcgccttgc acattcgagc
    11521 caaggggctg gttactggac cggtggatgt cagccccctt gaagcgctga tgcatgatgg
    11581 agaactgacc gatacgcaat ggttgcttgc ctatgaggca aatgtaaaag gctggcttcc
    11641 caacaaggcc ggaaaggact tcgtggcggc acacaagcat ttcgggccga tgaaagcggc
    11701 tggtgtgtcg ttttacgacg aatccgcgat gcttcctata gtggtaaagg ctgccgtcgt
    11761 gcagggctat ggagatgccg acgatgattt tgatctggcg tatttggttg gcaatgtttc
    11821 cgggtagcgc tgctcgtgtc tattgagttt tcgagcccct ctctagtcgt agccaacagg
    11881 gacattgccg ccctggttgt gtggtgctgt tccgggggta cgaatgtcgc caactggatg
    11941 attaatagcc ggtgacgcca acaagcctga gttccgggtg taggccggag gcggccatcg
    12001 tagcggcatg gcaaatagtg tagctataga tgttcagtgc gacgggcttg gtcggcaggt
    12061 gtttcacaag tacacttgtc tcgattgacg cttataggta gttctgctga cgcgctgacg
    12121 cttgtcacct gctcaaaccc gcaccgcttc gcggtgcctg ctttgagcag gtagaatgta
    12181 aagaactcgg cagttggcta taaattattt acattagaaa ggttgtgctg gatgaaaact
    12241 ggttttaata caagtgtttg ttgccttgtt tacggttttt gttccacaca accgttctga
    12301 cgcgaggaca tcatggccaa gaccttcacc aaactgaccc gaccgctca
//
LOCUS       DAOQLC010000123        12158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2005_length_12158_cov_25.112038, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000123 DAOQLC010000000
VERSION     DAOQLC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_12158_cov_25.112038"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PL000_17240"
     CDS             complement(<1..586)
                     /locus_tag="PL000_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90677.1"
                     /translation="MPINYQEIYTQVKQVGQGAKERRKKKEEAQELAQKLLETFSSEL
                     DALRSKVDSAKQADANIRCAVPLDEALASHYPTPDSVSATLIAADGSQAIADRHSAVQ
                     FCVINVGAISMKPDSGETPAVEVDSELFFGDAIEENGLTTDGGVALRRDLAERSVIEK
                     MSKGLKGSVVSFTDGTLEIFRAKDIDNANMYRNTV"
     gene            731..1453
                     /locus_tag="PL000_17245"
     CDS             731..1453
                     /locus_tag="PL000_17245"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNC90678.1"
                     /translation="MNKKILVFAILAFALILSACGPTTINQEAPAVVRTLNVNGLGQV
                     NLTPDIAYIYIGVHTEGATATEAVEANKTQTTALIASIKKSGVDEKDIRTTNFSIWPS
                     QQYGPDGTISGTVYMVDNTVNVTVRDLENLGDLLDSAISAGANTINSIQFDVADKVEA
                     LKEARAKAVADAQAQAQELAEAAGVSLGDIQTISFYENSVYPMDMGKGGGGAAYDAAS
                     AVPIQPGQLTVSVNVNMVYSIK"
     gene            1570..2508
                     /locus_tag="PL000_17250"
     CDS             1570..2508
                     /locus_tag="PL000_17250"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC90679.1"
                     /translation="MKAGRVIIPYHSQSRSHFMLRVGYIGLGLMGKSIARNILKAGFP
                     VVVHNRSRGAVDELVAEGAVAANSPKEVAEQVDVVFTNLPDSPDVEKVALGENGILAG
                     GRAGLIVVDNSTIKPASARLVAEKLKEKGIFSLDAPVSGGDIGAKNGTLTIMVGGEVE
                     ALEKVMPVFQAMGKTITHVGDAGAGQVAKAANQIMVAAQMVAMGELLVFSKKAGVDPR
                     KVVDAIKAGAAQCWALDIKPPRLFDGNRNPGFKSYMQLKDMKIVLDTAKEYGVPVTST
                     EENTRFYQMMIDMGLGELDNSAVVGVIEKLAGVEII"
     gene            2556..3098
                     /locus_tag="PL000_17255"
     CDS             2556..3098
                     /locus_tag="PL000_17255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNC90680.1"
                     /translation="MQINQPVPDFELPDLDGNLHRLSQNRGKIVIVNFWSCECPHSER
                     TDKAIMAMFVQHLHRAAENDAVTEWRDEVKMLSIASNRNEHVEALKTAAEARRLPTVL
                     LDADCRVADLFEAQTTPHVFVVDREGILRYCGAVDDATFRNRMPTRFFLDLAVEALLD
                     GQLPALTESPAYGCAIVREI"
     gene            3111..4919
                     /locus_tag="PL000_17260"
     CDS             3111..4919
                     /locus_tag="PL000_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC90681.1"
                     /translation="MIELKQRVMFLDKMHLFNGLKEEQLSGIGQKLTEREFSAGSVIF
                     KRGDKPDGFYMVYKGRVKVTRPRDVGEDFLAWLAPGDYFGEEALFENRNRSATITAME
                     NSTLLFLTREEFEKLLDRYDVLKPNFKVAIKSRKLARSTRFKWLGKNEVIYFIARRHK
                     LKLYQALVYPVLALAIPAGLFGLGVLTAATTPYALATVALVGILGWGVWNAIDWSNDY
                     YIVTNQRVIWLEKVIGLHDSREEAPLGTILSVGVETDAVGRVLDYGNVIVRTFVSRLE
                     FDYVDHPNQAADMIREYWERTKAITTRSQMDVMKDTIKQKLGLPLDKRPQVELSPVVP
                     EDQEKIAKTPYWILALSALFKLRVEEGATVIYRKHWIVYLQRGWRSLLTTLGVVFVFF
                     ARIWYFASSPTASFMKPNADGALRPDTLLLTIPVLMLPIGFWLWYEYTDWKNDIFKVT
                     SDEIIDLDRKPFGKEERRSAQIESILSTSYKREGLIANIFNYGTVNISVGGNQLAFED
                     VIDPAGVQADINRRRSARIAKKNEDAGKDDRERFATWLAAYHQNINEFTAPVKVVKED
                     TASTNQFTKFNPPGMEDMAAEDNEQDMGGTDMAGGE"
     gene            4991..5518
                     /gene="def"
                     /locus_tag="PL000_17265"
     CDS             4991..5518
                     /gene="def"
                     /locus_tag="PL000_17265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC90682.1"
                     /translation="MALREIVFLPEPVLRRKAKPVTKFDKELQTLIDDMIETMREAPG
                     VGLAAPQVNISQQLAVIEYDEDEDEEENEDAPPKPKKLYVIINPEIIKASEEKVMGIE
                     GCLSIPGLQGEVERHQSIQVKALNRHGSPVKLKVDGWMARIFQHEIDHLNGVLFTDRA
                     TRVWKPREDEDVPLD"
     gene            5532..5996
                     /locus_tag="PL000_17270"
     CDS             5532..5996
                     /locus_tag="PL000_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90683.1"
                     /translation="MSIRLLTPDDLPRLREFWIEHWGSDLMITRGESIRCDEVEGFVY
                     GEWLGLLTFLVRGDECEITSLDSLQAGQGIGSALLEAVLAEVKKRHCRRLFLITTNDN
                     LHALGFYQKRGFELAALYRGAVNQSRKFKPSIPLLGENNIPLRDEIELEMQV"
     gene            5993..7261
                     /gene="recF"
                     /locus_tag="PL000_17275"
     CDS             5993..7261
                     /gene="recF"
                     /locus_tag="PL000_17275"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNC90684.1"
                     /translation="MNGKTSFQPFQTTAFQPVTYNPMYLKHLSLTNFRSLTRLDTELP
                     RRAIVLVGSNAQGKTSVLEAIYFLAAFTSFQTHVDRQIVNFHEAKNPLAVTRLVADYQ
                     RGKSKHHLEARLILEPTGVNGQRLRKEILLDGVKRHASDVIGHFNAVIFVPQMSQIVE
                     GGPDERRRYLNLALAQTVPAYARALSEYNQALTQRNALLKALSERGGNSDQLQVWDEA
                     LVKFGAQIILWRIQAVQEIERFASRIHHELTRGAEILRLAYEPAYDPLPKPNGQLGLK
                     LDTTIDRSSIELGEIQDGFLARLKQIRSEEIARGVTTIGPHRDDLRFIINKADVSDYG
                     SRGQLRTTLLALKLAEVEWMKERTGEWPVILLDEVMAELDTSRRADLLKYVSPDAQVL
                     FTTTDLTLFAPEFVQSAEVWNVKGGVVTSK"
     gene            7328..8872
                     /locus_tag="PL000_17280"
     CDS             7328..8872
                     /locus_tag="PL000_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90685.1"
                     /translation="MGRIKRDDFQSYKTDFFAMSHPWVMLSVAILLVNDYVLKVYFPS
                     WLTGKLSDLAGLFFFPILLSAVLNIVLEPLKFQPRHIAFSSFWFTALWFTLIKTLPFF
                     SGLTGDILSSLMSLPVKIICDPTDMIALAMLFPAWKLRLSAGNGRMTKKRGLSYAMLF
                     IASLAAMASGPSGNIRVITRLVVHENKIYTYHDYSKDPYASNDPYASSSYEENYYSSD
                     GGQTWERSNETLPLQVVDQFMVPSKLPFTLCLPKAPNTCYRTGKETILESLDGGKTWN
                     VSWRIPLGREEFLSRILARHHLGPYDLVSLDYNGEQVVVAAIGLQGVLVKMSDAPWIS
                     VGVDGLGPIYFEARNINEAVDAIDEEIVALGLVAIVLFLVNAFFNVVRKGDQGVYFVA
                     VTLITFFGALGMLGLHFTPMGFTDASPPFLDGSSLLFLNGFAVVMFALQQILTGVGKQ
                     GARNLFVLFMTWLVSCSLFLLWAYGVIHVYETAKTIAVVLHVFSMLWVVYFFVRGPIQ
                     NKGPVL"
     gene            8980..9909
                     /gene="rbsK"
                     /locus_tag="PL000_17285"
     CDS             8980..9909
                     /gene="rbsK"
                     /locus_tag="PL000_17285"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HNC90686.1"
                     /translation="MSETGKYDVLVIGSLNADLVVRAPRFPQPGETISGDDLHTIPGG
                     KGANQAVAAARQGGRVAMLGRVGMDNFGDFLLENLKQNQVDVSNVQRDDASTGTAIIV
                     VDANGQNSIVLSAGANGHVSSADVDRASSLTPSLILLQLEIPSPTVLHAAQFAKANGA
                     RVILNPAPAQPLPDDLIRLADFIIPNETELSLLTGLEVKDSASAEAAAKALLKRGVEN
                     VIVTLGSKGALVLSDTRVMQVDAYPIDVVDTTAAGDAFIGGFASSLLRGLELKDAVRY
                     ANACGALAATKFGAQPSLPTKNEVETFMSLHDQ"
     gene            10036..11013
                     /locus_tag="PL000_17290"
     CDS             10036..11013
                     /locus_tag="PL000_17290"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNC90687.1"
                     /translation="MNPKRIIIDTDPGVDDALTFLLALASPEIQLEALTTVHGNTNVE
                     NTTRNALAVLEFLHAENIPVAKGCSLPLLVPLHKSGDMVHGAGGIGNTNLPEPKNKPV
                     DEHAIDYLIQRVLAEPKELSIFPIGPLTNIALAIRKEPRFAEAVKELVIMGGSLRAGG
                     NITPAAEFNLHADPHAAHIVFHSGIPITLIPLDVTYKCLLTSEDVERLNRTGSPIARF
                     VRDATGVYMEFYKKYEGFDGCALHDPLTLATIIAPELLTFENHYVDVDISGGISMGNT
                     LADFMKVSKKPANVKVALGVRGREFVELFIERMETLSASVGTHTAEDGG"
     gene            11040..11987
                     /locus_tag="PL000_17295"
     CDS             11040..11987
                     /locus_tag="PL000_17295"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HNC90688.1"
                     /translation="MPKHIILDTDPGIDDSLAILLALASPELVLDGISTIHGNASTAQ
                     VTRNALAILELAKAGHIPVYAGCELPLVQPSLLSPETHGDSGLGYAKLSEPLGRPQLK
                     HGSDFLIERIMSSVEDVTLVAIGPLTNVAMAIRKEPRIVEKVKEVFIMGGAIRHQGNT
                     TPLAEYNTFVDPHAAHIVFHSGMPMILTPLDVTYQCIFLKEDLDRLLKIDSPITKFIA
                     DATRFYMEFHDEYQKIDGCVINDPMTLALTFMPEICDYESLYVDVDISTGVGLGNTFA
                     DFYNYEKKKPNMKVALGVRPRMFMELFLERMEKLARLLE"
BASE COUNT         2893 a         3006 c         3284 g         2975 t
ORIGIN      
        1 ccacggtgtt gcggtacata ttggcattgt caatgtcttt agcacggaag atttcaagcg
       61 tgccgtctgt aaatgaaacg accgagcctt tcaagccttt gctcatcttt tcaataacgc
      121 tgcgctcggc aaggtcgcgc cgcagggcaa cgccgccgtc ggtggtgagt ccgttctctt
      181 cgatggcgtc gccgaagaag agctcgctat ccacttctac agctggggtt tcacctgaat
      241 caggtttcat agagattgcc cccacattga tgacacagaa ctgaaccgcg ctgtgtctgt
      301 ctgcaatggc ttgcgaacca tcagcggcga tgagagttgc agaaacagaa tcaggcgtcg
      361 ggtagtgaga agcgagggct tcatccagcg gcaccgcgca gcgaatattg gcatccgcct
      421 gtttggcaga atccactttc gagcgcagtg catccagttc agaagagaag gtttcgagga
      481 gtttctgcgc gagttcctgc gcctcctctt tcttctttct tctttccttc gcgccctgcc
      541 caacctgctt gacctgtgtg tagatttcct gatagttaat tggcatagaa catttgtgca
      601 aaattttagc cgtaaaccaa ccaaaataca aggtggtgcg gttctcttaa aaataaataa
      661 gtcatcccca gacgggaact ttttctgaag atgggtcgtc tgataggcat aacacttaag
      721 gagacataca atgaacaaga aaattttggt atttgcaata ttggccttcg ccctcattct
      781 gagcgcctgc ggcccaacca cgatcaatca ggaagcacct gccgttgttc gcacattaaa
      841 cgtcaacgga ctcggacagg tcaacctcac acctgatatc gcctatattt atattggtgt
      901 tcacacagaa ggtgcaaccg ccactgaagc agtggaagcg aacaagaccc aaaccactgc
      961 gctgatcgct tcgatcaaga aatcaggcgt ggatgaaaaa gatatccgca ccaccaattt
     1021 cagcatctgg ccttcacagc aatacggccc cgatggcacg atcagcggca ctgtttacat
     1081 ggtggataac acggtcaatg tgactgtgcg tgatcttgaa aacctcggtg acctgctcga
     1141 ttcagccatc agcgcgggtg ccaataccat caacagcatc cagttcgatg tagcggacaa
     1201 ggttgaggct ttgaaggaag cccgtgccaa agccgttgca gatgcgcagg ctcaggcgca
     1261 ggaactggcc gaagccgctg gcgtttcact cggcgacatt cagaccatca gcttctatga
     1321 aaatagcgtt taccccatgg atatgggcaa aggtggtggc ggtgcagcct atgatgcagc
     1381 ctcggctgtg ccgatccaac ctggtcagtt gacggtgtct gtgaacgtca acatggtgta
     1441 ctcgatcaaa taaacgcatc acccttaacc gaaacctgct cctgaacaag gagcaggttt
     1501 tacgtttcat cctcctctcc tgaatgcggc aagttgtggt tgcgtttggg agaggtgcct
     1561 ttttgggcgg tgaaggctgg gcgcgttata atcccgtatc attcccaatc aaggagtcac
     1621 tttatgttaa gagttggata catcggtctt ggattaatgg gcaaatccat cgcgcggaat
     1681 attctcaagg cgggttttcc tgtggtcgtg cataaccgtt cccgcggcgc agtggatgaa
     1741 ctggtcgctg aaggggccgt tgcggcaaat tcgccgaagg aagtggccga acaagtggat
     1801 gtggttttca ccaacctgcc cgattcaccc gatgtggaga aagtggcatt gggagaaaac
     1861 ggaatcctcg cgggtgggcg cgctggcctc attgtggtgg ataactccac catcaaacct
     1921 gcttctgcgc gtttggtcgc agagaagttg aaagagaagg gaatcttctc gttggatgcc
     1981 cctgtttcag gcggcgatat tggcgcgaag aacggcacac tgaccatcat ggtcggtggc
     2041 gaggttgaag cgctggagaa ggtgatgcct gtattccaag cgatgggcaa aaccatcaca
     2101 catgtgggtg atgcaggtgc cgggcaggtg gcgaaggccg ccaaccagat catggtcgcc
     2161 gcacagatgg tggcgatggg tgaattgctg gtcttttcca aaaaagctgg cgttgacccc
     2221 cgcaaagtag tggatgcgat caaagctggc gcagcacaat gctgggcgct tgatatcaaa
     2281 cccccgcgtt tgttcgatgg caaccgcaac cctggcttca aatcgtacat gcagttgaag
     2341 gatatgaaga tcgttttgga taccgcaaaa gaatacggtg tacccgtcac ctccaccgag
     2401 gaaaatacaa ggttctacca aatgatgatc gatatggggc tgggcgagct ggataactct
     2461 gccgtggtgg gtgtgatcga aaaactggcg ggtgttgaga tcatctaaac gttaacacgt
     2521 ttgaacgttc caacgtttaa aacctgtaaa ctcgaatgca aatcaaccag cctgtccctg
     2581 atttcgaact gcctgatctt gatggcaatc ttcaccgctt gagccagaat cgcgggaaga
     2641 ttgtcatcgt taatttctgg tcgtgtgagt gtccgcattc ggagcgcacc gacaaggcga
     2701 tcatggcgat gttcgtgcag cacttgcacc gcgctgccga aaacgatgca gtgacagagt
     2761 ggcgtgacga ggtaaaaatg ctgtccatcg cgtcgaatcg aaatgagcat gtggaggcgt
     2821 tgaaaactgc cgctgaggcc cgccgcctgc ccacggtcct gttggatgcg gattgccgcg
     2881 tggcagatct gtttgaggcg cagaccactc cccatgtgtt tgtggtggat cgggagggaa
     2941 tccttcgcta ctgtggcgca gtggatgatg ccacctttcg gaatcggatg cccacccgct
     3001 tctttctgga cctggcggtt gaggcattgc tcgatgggca gttgccagcg ctgacagaat
     3061 ctcccgccta tggctgcgcc attgtccgtg aaatttgata gaatcaaaac gtgattgagc
     3121 tcaaacagcg cgtgatgttt ttggataaga tgcacctctt caatgggttg aaagaggagc
     3181 agttatctgg cattggtcaa aaactgaccg agcgcgaatt ttctgcgggg agtgttattt
     3241 ttaagcgcgg cgataaaccc gatggctttt acatggtcta taaaggcagg gtcaaagtga
     3301 cccgtccgcg tgatgtgggg gaggattttc tggcgtggct ggcaccgggc gattattttg
     3361 gcgaagaagc cctgtttgaa aaccgaaatc gttcggccac catcaccgcc atggaaaata
     3421 gcaccctgct tttcctcacg cgtgaggaat ttgaaaaact gcttgaccgt tatgatgttc
     3481 tgaagccgaa tttcaaagtg gccatcaaga gccgcaagct ggcacgttcg acccgtttca
     3541 aatggctggg taagaacgaa gtcatttatt ttattgcccg cagacataaa ttgaagttgt
     3601 atcaggccct ggtgtatccg gtgcttgcgc tggcgatccc tgccgggttg tttggattgg
     3661 gtgtgttgac agccgccacg accccctatg cactggctac tgtggctttg gttggcatcc
     3721 ttggttgggg ggtgtggaat gccattgact ggagcaatga ctattacatc gtcaccaatc
     3781 aacgcgtgat ctggttggag aaagtgatcg gcctgcatga cagccgcgaa gaagcgccgt
     3841 tgggcacgat cctttctgtg ggtgtggaaa ccgatgcggt tggccgtgtg ctggattatg
     3901 gcaatgtgat cgtgcgtacc tttgtcagca ggcttgaatt cgattatgtc gaccacccca
     3961 atcaggctgc agatatgatc cgcgaatatt gggaacgcac aaaagccatc accacccgca
     4021 gccagatgga tgtgatgaag gacaccatca aacaaaaatt gggcctgccc ttggataaac
     4081 gtccgcaggt ggagctttct ccggtggtgc ccgaagatca ggaaaagatc gcaaaaacac
     4141 cttattggat tcttgcgctc tccgcccttt ttaaattacg tgtcgaggaa ggcgccaccg
     4201 tcatttaccg caaacattgg atcgtgtact tgcaaagagg ctggcgttca ctgttgacca
     4261 cgctcggtgt cgtttttgtg ttctttgccc gcatctggta ttttgcgtcc tcgccgaccg
     4321 catcctttat gaagccgaat gccgatggtg cgctcaggcc cgatacgctt ttgttgacca
     4381 ttccggtgtt gatgctgccg atcggttttt ggctatggta tgaatacacc gactggaaga
     4441 acgatatctt caaagtcaca tccgatgaga tcatagatct tgaccgcaag ccttttggta
     4501 aagaggaacg ccgctctgcc cagatcgaaa gtattttgag cacctcttat aaacgcgaag
     4561 gattgatcgc caacatcttt aactatggca cggtcaatat ttcggtgggc ggcaaccaac
     4621 tggcctttga agatgtgatc gaccctgccg gggttcaggc ggatatcaac cgcaggcgtt
     4681 cggcacgtat tgccaaaaag aatgaagatg caggcaagga tgaccgcgaa cgctttgcca
     4741 cctggctggc ggcctatcat cagaacatca acgaattcac cgctccggtg aaggtggtaa
     4801 aggaagatac cgcctccaca aaccaattta caaaattcaa tccgcccggc atggaagaca
     4861 tggccgccga agataacgaa caggatatgg gcgggactga catggcgggt ggggaatagc
     4921 ctgcaatgtg cggtatcatt gcggctcaaa atgaccaaag acaagtttta gcaaaaagga
     4981 agaccaaaga atggctttaa gagaaatcgt attcctgccc gagcctgtat tgcggcgcaa
     5041 ggccaaaccc gtgacgaaat tcgacaagga attgcaaacc ctcatcgatg acatgatcga
     5101 aaccatgcgc gaggcaccgg gcgtcggcct ggccgctccg caggtgaaca tttcgcagca
     5161 gctggctgtg atcgaatatg acgaggatga agacgaagag gaaaatgaag atgcccctcc
     5221 gaagccgaag aagttgtatg tcatcatcaa cccggagatc atcaaggctt ctgaagaaaa
     5281 ggtgatgggg attgaaggct gcctttcgat tcccggcttg caaggcgaag tggaacgaca
     5341 tcagtccatt caagtgaagg cgctcaaccg ccatggcagc cctgtcaaac tcaaggtgga
     5401 tggctggatg gcgcgcattt tccaacatga gatcgaccat ctgaacggag tcctcttcac
     5461 agaccgtgcc acccgcgtct ggaagccaag agaggatgag gacgttccgc tcgattagca
     5521 attccctgaa aatgagcatc cgcctcctca ctcccgatga cctgccccgc ctacgagaat
     5581 tttggatcga acactggggc agtgacctga tgatcacacg cggcgaatct atccgttgtg
     5641 atgaagtgga ggggtttgtc tacggtgagt ggctcggcct gttgaccttt cttgtccgcg
     5701 gtgatgaatg tgagattact tcgctcgata gtttgcaggc tgggcaggga attggctcag
     5761 ccctgctgga agcagttctg gcagaagtaa aaaagagaca ttgccgccgt ctgtttctca
     5821 tcaccaccaa cgacaacctg cacgcgctcg gcttttacca aaaacgtgga ttcgaactgg
     5881 cggccctgta tcgcggtgcg gtcaaccaat cgaggaagtt taaaccgtcc attccattgc
     5941 ttggagaaaa caatattccc ctccgcgatg agatcgaact tgagatgcaa gtttgaatgg
     6001 caagacaagt tttcagcctt tccaaaccac cgccttccaa cccgtaactt acaacccgat
     6061 gtacctcaaa cacctctccc ttaccaactt tcgcagtctc acccgcctgg atactgaact
     6121 tccacggcgg gcaattgtgt tggtggggag caatgcgcaa ggcaaaacca gcgtgcttga
     6181 ggcgatctat tttctggcgg cattcacttc cttccaaacc catgtggacc ggcagatcgt
     6241 caattttcac gaagcaaaga atcccctcgc tgtgacccgc cttgtggcag attaccagcg
     6301 tggaaaatcg aaacatcatc tcgaagcgcg gctgatcctt gaacccactg gcgtgaatgg
     6361 gcaaagactc cgcaaagaga ttctgctgga tggggttaag cgtcacgcca gcgatgtcat
     6421 cgggcatttc aacgcggtca tttttgtgcc gcagatgtcg cagatcgtgg agggcggccc
     6481 cgatgaacgc agacggtatc ttaatctcgc cctggcacaa actgttcctg cgtatgcgcg
     6541 ggcgctcagt gaatacaatc aggcgttgac ccagcgtaac gccctgctca aggcgctgag
     6601 tgaacgagga ggaaacagtg accagcttca ggtgtgggat gaagcgttgg tcaaattcgg
     6661 ggcgcagatc attttgtggc ggattcaagc cgtgcaggag atcgagcgtt tcgcgtcgcg
     6721 catccatcac gaattgactc gcggtgcgga gattttgcgc cttgcctatg aacccgccta
     6781 cgaccccctg cccaaaccga atggacaact gggcttgaaa ctggatacaa ccattgaccg
     6841 ttcatccatt gagttgggtg aaattcagga tggattcttg gcgcggctca aacagatccg
     6901 cagtgaagag atcgcgcgtg gcgtgaccac gatcggcccg catcgcgatg acctgcgatt
     6961 catcatcaac aaagcagatg tgagcgatta cggttcgcgt ggacaactcc gcacgaccct
     7021 gctcgcgctt aaactcgccg aagtggaatg gatgaaggaa cgcaccggtg aatggcctgt
     7081 gattctgctc gatgaagtga tggccgaact tgataccagt cgccgtgctg acctgctgaa
     7141 atacgtcagc cccgatgcac aggtgttgtt taccacaacg gatttgactt tgttcgcgcc
     7201 ggagtttgtg caaagcgcgg aggtttggaa tgtgaagggt ggtgtggtta cgtcgaagta
     7261 gcaatgaggt tattttcata attaactgaa aatgggtgaa atcaagaaaa tgggataggt
     7321 gatgacaatg ggcaggatta aacgcgatga ttttcaaagt tataaaaccg atttttttgc
     7381 catgagccat ccatgggtga tgctgagtgt tgccattttg ttggtcaacg actatgtctt
     7441 gaaggtatat tttccttcct ggcttaccgg caaactcagc gaccttgcgg ggctgttttt
     7501 ctttcccatt ctcttgagtg cagtattgaa tattgttctt gagccgttga aatttcaacc
     7561 acgtcacatt gctttttcgt ccttttggtt taccgcatta tggtttactt taatcaaaac
     7621 cctgccgttc ttcagcggcc tgactgggga tattctttcc tctctgatgt cgctccctgt
     7681 aaaaattata tgtgacccga ctgacatgat tgcattagca atgctttttc ctgcatggaa
     7741 attgcgattg agcgcaggga atggcaggat gacaaaaaag cgcgggctgt catatgcaat
     7801 gttgtttata gcatcccttg cagccatggc ttctggccct agtggcaaca ttagggtaat
     7861 aacgcgcctt gttgttcatg aaaataaaat ctatacgtat catgactact ctaaagaccc
     7921 ctacgcctct aatgacccat acgccagcag tagttatgaa gagaattatt acagctctga
     7981 tggcgggcag acatgggaaa gatcgaacga aacactccct ttgcaagtcg ttgatcaatt
     8041 tatggttccc tcaaaattgc ctttcacgtt gtgcctgccg aaagcaccga acacgtgtta
     8101 ccgcacaggc aaagaaacga ttctcgaatc ccttgatggc ggaaaaacat ggaatgttag
     8161 ttggcgcatt ccattgggca gggaggagtt tttgtctcgc attctggcaa ggcatcatct
     8221 tgggccgtat gatctggtct ctcttgatta caatggagaa caggtggttg ttgcggccat
     8281 tggtttgcaa ggcgtcctag tcaaaatgag cgatgctcct tggatttctg ttggggtgga
     8341 cgggttgggt ccaatttatt ttgaggcaag gaatataaac gaggcagtgg atgccataga
     8401 tgaagagatc gtcgccctcg gcctggtggc tatcgtgctt tttctggtca atgcgttttt
     8461 taatgtcgtg agaaagggcg atcagggtgt ttacttcgta gcagtaacat tgattacgtt
     8521 ttttggtgcg ttaggtatgc taggacttca cttcaccccc atgggcttta ccgacgcgag
     8581 cccgcctttc ctagacggtt cttccttgct ttttttgaat gggtttgccg tggtcatgtt
     8641 tgccctccag cagatactca ctggagtggg aaaacaaggc gcaagaaatc ttttcgtgct
     8701 tttcatgacc tggctggttt cctgctcgct ttttctcctt tgggcatacg gcgttattca
     8761 cgtgtatgaa actgcgaaaa caatagccgt tgttttacat gtgttttcta tgttgtgggt
     8821 tgtctatttt tttgtcaggg gaccaattca aaataaagga ccagttttgt agcttcgcgt
     8881 gaaatacact gtgagtacat agatataatg ctcccaatga ttcaattttt cgatgccgct
     8941 ttcgacatca cgcgataacg atagtgtaaa attgcccgta tgtctgaaac agggaaatac
     9001 gatgttcttg tcatcggttc gttgaacgcc gacctcgtcg tccgtgcgcc gcgatttccg
     9061 cagcccggcg aaaccatcag cggtgatgac ttgcacacca ttcctggcgg caagggggcc
     9121 aatcaggctg ttgctgcagc gcggcagggt ggcagggtgg ccatgttggg gcgcgtgggc
     9181 atggataact tcggcgattt cctgctcgaa aacctcaagc aaaaccaagt ggatgtttcc
     9241 aatgtccagc gtgatgatgc ctccacggga actgccatta tcgtggtgga tgcgaacggg
     9301 cagaacagca tcgtgctatc tgctggagcg aacggtcatg tctcttctgc ggatgtagat
     9361 cgcgcttctt ccttaacccc gagcctgatt ttgcttcaat tggaaatccc ctccccgacg
     9421 gtgcttcacg ctgcgcaatt tgccaaagcg aacggtgcgc gtgtcatcct caacccggcg
     9481 cctgcacaac ccctccccga tgacttgatc cgccttgcag atttcatcat tcccaatgaa
     9541 accgaattaa gcctgttgac cgggcttgag gtcaaagaca gcgcctctgc cgaagcggct
     9601 gcgaaagccc tgctcaaacg cggcgtagag aatgtcattg ttacgttggg aagcaaaggg
     9661 gcattggtct tatccgatac acgggtgatg caggtggatg cctatccaat tgacgttgtc
     9721 gataccacag ccgcaggcga tgccttcatc ggcggattcg cctcatctct cctgcgcgga
     9781 cttgaactca aagatgcggt tcgatatgca aatgcctgtg gcgcgctcgc tgcaaccaaa
     9841 ttcggcgcac aaccatccct gccaacaaaa aacgaagtgg aaacctttat gtcattgcat
     9901 gaccagtgac ctttcgcccg aagcgatctc atcgcggccg ttgagaccgc tcaccacatg
     9961 aactgcctgt caattcatgt gcggcgcaag tgttcccaga tcatcgctct cctcgcaatg
    10021 acggggaaaa aataaatgaa cccaaaacga atcatcatag acaccgaccc cggcgtagac
    10081 gacgcgctga catttctcct tgcccttgct tcccctgaaa tccagctcga ggcattgacc
    10141 accgtccacg gtaacacgaa tgtggaaaat accacgcgca atgcgctggc tgtattggaa
    10201 ttccttcacg cggaaaatat ccctgtggcg aagggatgtt cgttgccgtt gctcgttcca
    10261 ctgcacaaat caggtgacat ggtgcatggt gcgggtggaa tcggcaatac aaacctgcca
    10321 gaacccaaaa acaaacccgt tgacgaacat gcgattgatt atttgatcca gcgtgtgctg
    10381 gccgagccga aggaactcag catttttccc atcggcccgt tgaccaatat tgcgctggcc
    10441 attcgcaaag agccgcgttt tgccgaagcc gtgaaggaac tggtcatcat gggtggttct
    10501 ctgcgtgcgg gcggtaatat cactcctgct gctgaattca acctgcatgc cgacccgcat
    10561 gccgcgcata tcgtttttca ctcgggaatt cccattacgt tgatcccgtt ggatgtgacc
    10621 tacaaatgcc tgctcacttc tgaagatgtg gagcgtttga acagaacggg ctcgcccatt
    10681 gcccgatttg tccgcgatgc gacgggggtt tacatggagt tttacaagaa atacgagggc
    10741 ttcgatggtt gtgcgctgca cgatccgctg acattggcga cgatcattgc gcccgaactg
    10801 ctgaccttcg agaatcatta cgtggatgtg gatatttctg gcggaatttc gatggggaac
    10861 acactggccg atttcatgaa ggtttcgaag aaacccgcaa atgtgaaggt tgccctcggt
    10921 gtgcgtgggc gcgaatttgt ggaattattt attgagcgga tggaaactct gtctgcttcc
    10981 gtcggcaccc acaccgccga ggacggcggc tgagcatagg aaggtacaga gaaaaaatca
    11041 tgcccaaaca catcatttta gacaccgacc ccggtattga cgactctttg gcgatcttgc
    11101 tcgctctggc ctcgcccgaa cttgtcctcg atggaatcag cacgatccac gggaatgcat
    11161 ccacggcgca ggtgacgagg aatgcgctgg cgattttgga actggcaaag gcggggcaca
    11221 tccctgttta tgcgggctgt gaattgccgc ttgtgcagcc gtccctgctc agcccggaga
    11281 ctcacggcga cagcggcctg ggttatgcaa aactgtcaga accgctgggc aggccccagc
    11341 ttaagcacgg aagcgatttc ctcatcgaga ggatcatgtc gtcggtggag gatgtcacgt
    11401 tggtggcgat cggtccgctg acgaatgtcg ccatggcgat tcgcaaggag ccgcgcattg
    11461 tggagaaggt gaaggaggtt ttcatcatgg gcggggcgat tcggcatcag ggcaatacca
    11521 cgccgctggc ggagtacaac acctttgttg acccgcatgc cgcgcacatc gtttttcatt
    11581 cgggcatgcc gatgatcctc acgccgctgg atgtaaccta tcaatgtatt tttctaaaag
    11641 aggatctgga caggttgtta aagatcgatt cgccgatcac gaagttcatc gcggatgcga
    11701 cccgctttta catggagttc cacgatgaat accaaaagat agatggctgc gtgatcaatg
    11761 acccgatgac tctggcgttg acgttcatgc ctgagatctg cgattacgaa tctctttatg
    11821 tggatgtgga tatctcgaca ggcgttgggc tgggaaatac gttcgctgat ttttataact
    11881 acgaaaagaa gaagccgaat atgaaggtgg ccctgggggt tcgtccgcgc atgttcatgg
    11941 agttgttttt ggagcggatg gaaaagctgg cgaggttgtt ggaatgacga tctttcacca
    12001 cagagcccac aggggccaca gagaaaaaaa agactccgtg ctctttgtgc tctctgtggt
    12061 taagtcttta aactgccagc ctatattttc gtgaccattg agattgaaaa gtgttatgat
    12121 gttcatacag atccattcac attttttcgg aggagatt
//
LOCUS       DAOQLC010000124        12044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2027_length_12044_cov_22.161315, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000124 DAOQLC010000000
VERSION     DAOQLC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_12044_cov_22.161315"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..806)
                     /locus_tag="PL000_17300"
     CDS             complement(51..806)
                     /locus_tag="PL000_17300"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNC90689.1"
                     /translation="MITTYHRPSTLDEALALLSQPNTVPLGGGTLLSQPKTAPVSVVD
                     LQRLGLDSIRINGNPSTGSGQALQIGATCTLQTLLEAADCPKALQTALKLEAPINIRN
                     TATAAGTLVAGDGRSTFVTALLALDAKITIVSGQSSVVSVGEYILTHSQGLITQIAVP
                     LNAKFAFEFVSKTPADKPLICAALTQWNSGRTRLALGGYGASPLLAMDGTEADGLDTA
                     ARNAYHEANDEWASAEYRMDVAATLAKRCLESL"
     gene            complement(849..2189)
                     /locus_tag="PL000_17305"
     CDS             complement(849..2189)
                     /locus_tag="PL000_17305"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90690.1"
                     /translation="MKDKNSLNAEERALAEMLAESAAQVEIGASFQNELEKKLMNAHK
                     PKTGFGLFSFGRAASTLASVLGLAILSLIFIWAIRAISSKPQPAIGNPFAVECPVTIP
                     NGSTPPGETATYEGYLGNGKLWTSLWPEGKIIMTPENRNADGSLSMKWLWWRGVSGPL
                     SIEGRRLDAEAEPLRSFISSGYGDIDFQVSELIFPTTGCWEVTGYAGNASLTFVTEVI
                     FEAATPTLIPTIAAQSTPISPTATFEVLPTASSTLFRDDFEGTLGNGWEWTHENSEQW
                     NLTDNGWLEIMVSYGIINNSNMDNLLLRQAPAGNFELETKLIFAPMEAPQMAGLLIYE
                     NNANYVELVHGACLTQPCLGDTFYFDQMTRGSFIEENFATPVFGTGAIHLRLRFEGDS
                     FTAYASENGTKWKLIGTHSRKIQPLYIGLFAGGSFDRAAQIPAQFDYFIVNALP"
     gene            complement(2186..2770)
                     /locus_tag="PL000_17310"
     CDS             complement(2186..2770)
                     /locus_tag="PL000_17310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC90691.1"
                     /translation="MTTTMTTHPHSPPADEVDLARLARTNAEAFGELYRRHVTRVYRY
                     HMAHTGNIKDAEDLTSQTFIAALENIGSFRGTGSFAAWVLGIAARKKAMFFRRIGTRP
                     EVQMEAADQIPNPGLPTEQLANQRLRLDSITRALKQISPERADAITLCFFSELTYAEV
                     GIALNKSEAAAKMLVIRGLQDLRERTSLAFEVEP"
     gene            complement(2825..3415)
                     /locus_tag="PL000_17315"
     CDS             complement(2825..3415)
                     /locus_tag="PL000_17315"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNC90692.1"
                     /translation="MIILTYLAVGASAISGALEARKHEMDIVGATTIAFVTAFGGGTI
                     RDLLLGRTPVFWVIDPWLTVVTFLVGAIAFYRLDRISNRLLIIADAIGLGLFSILGAT
                     YTLQLELSPIVAVLMGVVTGMFGGVLRDLLCNRIPNVFRQSTELYATCSFIGTSVFIL
                     LRSFTVGSFTASVIGAAIIFTMRLAAVRFKVTLPAP"
     gene            complement(3433..3909)
                     /locus_tag="PL000_17320"
     CDS             complement(3433..3909)
                     /locus_tag="PL000_17320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC90693.1"
                     /translation="MSKIKQPNSRHCFICGLENPVGLHLHMYEVEPGVIETQYIAPDH
                     FQGYPGVLHGGIVAALIDEISGRAQMGSDPANPRFMFTGKLEVKYRKNVPINKLLKIV
                     GKAGKSKRNIAEAWAGIYDAETNELLAEGSTVSVDVPKDQFEVSKLEELGWKVYPD"
     gene            3932..4900
                     /locus_tag="PL000_17325"
     CDS             3932..4900
                     /locus_tag="PL000_17325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNC90694.1"
                     /translation="MNQPKIFITRVIPSPGLNLVREHFLPVAWADEMPPTREQLLAHV
                     RGVDGLLCLLTEKIDGELMDAAGPQLKVISSMSVGVDHIDVAAATARGIPVGNTPGVL
                     TDATADQAFALMLAAARRVTEAERFLRAGKWVTWQPSLLLGADLVGATLGIVGFGRIG
                     QAIAKRAQGFDLRVLYHSPNAQPAYGAKPVDLDTLLRESDFVSINVPLKAETRHLVNA
                     DFLAKMKPNAILVNTARGGVLDQTALYDALKSKRIFAAALDVTDPEPLPMDCPLLELE
                     NCIIVPHLGSASQKTRDMMSLLAAQNLIAGLKGERLTHCVNPQVYG"
     gene            5164..5901
                     /locus_tag="PL000_17330"
     CDS             5164..5901
                     /locus_tag="PL000_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC90695.1"
                     /translation="MLEVDLHPKNTPVNPPDSDLVLLREVVKTYHGSAGTFTALKGVD
                     LRVESGAFVSIIGKSGSGKSTLINVITGIDRPSSGEVIVDRTPVHTLNEEQIAIWRGR
                     SVGVIFQFFQLLPTLTAVENILLAMDYGKHYPRLERPERAMQLLELVGMADYAHRLPG
                     SLSGGQQQCVAIARALANDPVLLTADEPTGNLDSKSTEMVFQLFENLAGTGKAILMVT
                     HDNELAVRAQRTIRLVDGRIQDEFVNR"
     gene            5882..6568
                     /locus_tag="PL000_17335"
     CDS             5882..6568
                     /locus_tag="PL000_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC90696.1"
                     /translation="MNSSTDKPLIELRSVVKVYESLAGSVTALNGIDLQVFQGEFLVV
                     TGKSGSGKTTLVNMLTGLDRSTSGEIWVNGEPLHKFGPEKAARLRGRTMGVIFQTFEL
                     LPTLTVLQNVMLPMDFARRYSRWEQRKRAYHLLEQVGIADHARKLPTALSGGQQQRLA
                     IARAMANDPAILVADEPTGSLDSATANAVVDIFAGLVQEGGTVFLVTHDKDIAKFGSR
                     VITLSDGEIV"
     gene            6581..8989
                     /locus_tag="PL000_17340"
     CDS             6581..8989
                     /locus_tag="PL000_17340"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90697.1"
                     /translation="MLRARWYKVINDLFGNKTRTLLIVLSMSVGLFALGIILSARTIL
                     ADGLAESFATIYPSSGTVRTIELFGEDFIESVRKMDDVQEADARRSVSVRMQTKSGEW
                     KNLTIFVFADYDDIRVNKVTSQSGAWPPPEREILIERAALSVIEAQVGDVILIRFPND
                     VERRIRIAGLAYDPAQLPAQIDGTPYGYITFDTLEWFGESYGFNELHVIAVNPEDKDW
                     AQQVVNRVKDKAENSGYTIPLSMTAEPGQLPMNDVLQGILLLMGLLGVLSLFLSIFLV
                     VNTVSALLTQQKRQIGVMKAVGGGTLQILGMYLVMVFSYGVLALLIAIPFGMYGAREL
                     SSVLAVFFNFDLYSMDVPPQTYLIQAAVGLILPVLASLVPFLSNLRISAAEAMSSYTM
                     GRGRFGKNWIDQLLSGANLWFARMLSIRSILLSVRNTFRNKGRLTLTLITLTLGSATF
                     ISVFNVRASLSSTVEDMIKWFNCDTMFALDRSYRADKVQREAESIPGVTQTDVWIQLP
                     TRMIRRDGSESGMIYMFAPTVRPDSLIVSPSLAEGRWLAPGDENAVVVPSALLNDEPE
                     LELGGDIVLKIDGDEHVFKIVGTFKGMAFLPIMYVNYDYITKITNRIGQADAVMVATQ
                     QHDAAYVDAVTRTLEERFNRVGVEIGLATTINTERRDAEASFDSIVALLLLMAILLAL
                     VGGLGLMGTMSINVLERTREIGVLRAIGAPNRSVASVFILEGIIIGLMSWAMGAAVAI
                     PMSRGLNQALGQAVMGVPLTYSYSLPGLWLWLVIVILLSVLASFIPARTASRLTVREV
                     LAYE"
     gene            9050..10282
                     /locus_tag="PL000_17345"
     CDS             9050..10282
                     /locus_tag="PL000_17345"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC90698.1"
                     /translation="MFNKLSLTFHRFPRTFWVVAGTHFIDVIGNTLLMPFFALYVTQK
                     FGVGMTEAGIILATNSVAGLIGSTIGGAMADRFGRRGIILFGLIVSAFSGLTLGFVNQ
                     FYMFYGLSVFVGLMGSAAHPAHNAMIADILPAEQRAEGFGIMRVISNFAWIIGPTIGG
                     FLAAYNFLYLFLSDAVISSITAVLVFRLIPETKPQPKEKTEHETLAQTIGGYRVALFD
                     RPFLAFIFASILMILVYQQMYSSLSVYLRDVHNVNPRFYGLVMSSSAVTVVIFQFWVT
                     SIIKKYPPFLMMATATFFYMIGFGMYGFVSTIPLFILAMVIITIGEMVGMPTSNVLAA
                     SFAPEEMRARYMAVFGLTWMVPSMIGPWAAGLIFDNMNPNYVWYISIVLCLIAMLAFY
                     LLQVRLGGREQFQRAKDG"
     gene            complement(10349..11041)
                     /locus_tag="PL000_17350"
     CDS             complement(10349..11041)
                     /locus_tag="PL000_17350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNC90699.1"
                     /translation="MTQLTGRERAAYVQNMFTQIAKRYDLMNRLMTGGQDIRWRKMVI
                     KLTRMTNTASLLDLGTGTGDLAREALTQFPQARITASDFTLEMMRVGKKNGPLNFSTA
                     DALHLPFNSTTFDAVISGFLMRNVINLDKAIEEQYRVLKNGGRIVILDTTRPKKNILS
                     PFIWIHMHIIIPTLGGLLTGVREAYRYLPETTEGFVTAEDMASRMSKAGFQNVNFKRL
                     MFGTIAIHWAEK"
     gene            11112..12005
                     /locus_tag="PL000_17355"
     CDS             11112..12005
                     /locus_tag="PL000_17355"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HNC90700.1"
                     /translation="MTEQTFNWQAWGLAIRPRTLPAAAAGVVMGGALAWHDGFFRLDA
                     TLVCLFAALLLQIGSNLANDVFDFERGTDTPERLGPTRVTQAGLLTSSQVKLGMAVVF
                     VLSALMGLYLIWLGGWVILAIGIAAIISAIAYTGGPFPLGYYGLGDVFVFIFFGLASV
                     AGTYYVQAGDVSSAAWWMTLPPGLIITAILVVNNLRDLENDRKAGKYTLAVMFGEQFA
                     KAEYLTCIFAAYLILPVAAWFGLIPWLSLMAWASFPIAFRAIRTVFTQKGRPLNAALA
                     GTGQAAFMFSLLFWIGLALQR"
BASE COUNT         2561 a         2967 c         3412 g         3104 t
ORIGIN      
        1 aaggaaaaac acaaaaggcc tcgcaatgag gccttttgtg tgtgacagac ttataaactt
       61 tccagacagc gttttgccag ggttgcggcg acatccatgc ggtattcggc ggaggcccat
      121 tcgtcgttgg cttcgtggta ggcgttgcgg gcggcggtgt cgagtccgtc ggcttcggtg
      181 ccgtccatcg cgagcagtgg acttgcgcca tagccgccga gggcgagccg tgtgcggcct
      241 gagttccatt gggtcagcgc ggcgcagatg agcggtttgt cggcgggggt tttggagacg
      301 aactcaaaag cgaattttgc gttaagcggg actgcgattt gggtaatcaa tccctgtgag
      361 tgggtcagga tgtattcgcc aacgctgacg accgatgatt gtccgctcac gatggtgatc
      421 tttgcatcaa gggcaagcag agccgttaca aaagtggatc gtccatcacc cgccaccaat
      481 gtccctgcag cggttgctgt gttgcggata ttgatcggcg cttcgagttt gagcgcggtt
      541 tgcagagctt tggggcaatc cgcggcttcg agcagggttt gcagggtgca ggttgcgccg
      601 atttgcaggg cctgccctga gcctgtcgaa gggttgccgt tgatgcggat cgaatccagc
      661 ccaaggcgct gaaggtctac aacggaaaca ggagcagttt tcggctggga gagaagcgtg
      721 cctccgccga gggggacggt attcggttga gagagaagcg cgagggcttc gtctaaggtc
      781 gaaggtctgt gataggttgt gatcattgca tctccttgat ggccctatta tacaatgcaa
      841 ggaaggcatt acggcagcgc attgacgatg aaataatcga attgagcggg gatctgtgcc
      901 gcacggtcga atgacccgcc agcgaacagg ccgatgtaaa gcggctgtat ttttcgcgaa
      961 tgggtgccga tcagtttcca ttttgtgccg ttttcgctgg cataagctgt aaaggaatct
     1021 ccctcaaacc gtaagcgaag atggatggcg cctgtaccga agactggggt tgcgaaattc
     1081 tcttcgataa aactgccgcg ggtcatttga tcgaaataga aagtgtcgcc gaggcatggc
     1141 tgagtgaggc aggctccatg cacgagttcg acataatttg cgttgttttc gtagatcagc
     1201 aaccccgcca tttgtggtgc ttccatcgga gcgaagatta gtttggtttc caattcaaaa
     1261 ttgcctgcgg gggcctgacg caaaagcaaa ttgtccatgt tgctgttgtt gatgatgcca
     1321 taactgacca tgatctcaag ccagccgttg tcggttagat tccattgctc gctgttctcg
     1381 tgtgtccatt cccagccgtt gcccagtgta ccttcgaaat catccctgaa taaagtggat
     1441 gaggcggtgg gcaaaacttc aaatgtcgcc gtgggtgaga tgggggtact ttgtgccgcg
     1501 atggttggga ttagtgtagg cgttgccgct tcgaagatga cttcggtaac gaatgtaagc
     1561 gaggcgttgc ctgcgtaacc agtgacttcc caacagcctg tggtggggaa gatcagttct
     1621 gagacctgga aatcaatatc tccatatccc gacgagatga atgaacgtaa gggttcggct
     1681 tctgcatcca ggcggcggcc ttcgatgctt aacggtccac tgacgccgcg ccaccagagc
     1741 catttcatgc tgagcgaccc atcggcgttg cgattctcgg gagtcatgat aatttttcct
     1801 tcaggccaga ggctggtcca caatttacca ttcccgagat acccttcata ggttgcagtt
     1861 tcgcctggtg gagtgctgcc atttggtatg gtgacagggc actcaacggc aaaaggattt
     1921 ccaatggcgg gttgaggttt ggatgaaatt gcgcggatgg cccagataaa tatcaaagaa
     1981 agtatggcaa gccccagcac cgaagccagt gtggaggcag ctctgccgaa tgaaaataaa
     2041 ccaaaccctg ttttgggttt atgggcgttc atgagttttt tctccagttc gttttgaaac
     2101 gaagcgccga tttccacctg tgcggcgctt tctgcgagca tttcagccaa cgcgcgttct
     2161 tcagcgttca gcgagttttt gtctttcatg gttccacctc aaaggcgagc gaggtccgtt
     2221 cgcgcaggtc ttgcagcccg cggatgacga gcatttttgc ggcggcttca cttttattga
     2281 gcgcaatgcc aacctcggcg taagtgagct cactgaaaaa acaaagggtg atggcgtctg
     2341 cccgctcggg gctgatctgt ttcagggcgc gggtgatgga atcaagcctg aggcgctgat
     2401 tcgccaattg ctcggttggc aggcctgggt ttggaatttg atccgccgct tccatctgga
     2461 cctctggtct ggttccgatt cttcggaaga acatcgcctt ctttcgggct gcaatcccca
     2521 aaacccatgc cgcaaaggag ccagttccgc gaaatgagcc aatgttttcg agtgcagcga
     2581 tgaaggtctg tgaggtgagg tcttccgcat ctttgatgtt gcccgtgtgc gccatgtgat
     2641 agcgatagac gcgtgttaca tggcggcgat acagttcgcc aaaggcttct gcatttgtgc
     2701 gggccaaacg ggcaaggtca acttcatcag caggcgggct gtgtggatga gttgtcatgg
     2761 tcgtggtcat ggacctcctg ttacgtgtat gttgcaggag gtatggaaaa agtaacaggg
     2821 aattttatgg tgcggggagt gtcactttga agcggacggc tgcgaggcgc atggtaaaaa
     2881 tgatcgccgc gccgatcaca gatgccgtaa atgatccaac ggtgaatgac ctgagcaaaa
     2941 taaaaacact ggtgccgatg aaggagcagg tggcatacaa ctctgtgctt tgacggaaca
     3001 catttgggat gcggttgcaa agcaggtcgc gcaatacacc gccgaacatg ccagtgacca
     3061 cgcccatcaa cactgccacg atgggcgaaa gctcaagctg cagggtgtac gttgccccga
     3121 gaatgctgaa caaaccaagc ccgatcgcat cagcaatgat gagcagtcgg tttgaaattc
     3181 tatcgaggcg gtaaaatgcg atcgccccca ctaaaaacgt gaccacggtc agccatgggt
     3241 caatgaccca aaaaacgggt gtgcgtccca gcagcaggtc gcgaatcgtc ccgccgccga
     3301 acgctgtgac aaacgcaatg gtggtggccc cgacgatgtc catttcatgt ttgcgcgctt
     3361 cgagcgcacc cgaaatggcc gaagcgccca cagccagata tgtgaggatg atcaaaatat
     3421 tttccacgaa gattaatcgg ggtaaacctt ccagcccaat tcttcgagtt ttgaaacttc
     3481 gaactggtcc ttcggcacat ccactgaaac cgtcgaacct tcggcgagca gttcgttcgt
     3541 ttcggcgtcg taaatccccg cccatgcttc ggcgatgttt cgtttgcttt tgcccgcttt
     3601 gccgacgatc ttcaatagct tgttgatcgg cacattcttg cgatacttca cttcaagctt
     3661 tcccgtaaac atgaagcgcg gattggcagg gtcgctgccc atttgcgcgc gtcctgagat
     3721 ctcatcgatc aatgcggcca caatgccgcc gtgcagcacg cccgggtaac cttgaaaatg
     3781 atcgggcgcg atgtactgcg tttcaataac gcctggttcc acttcgtaca tgtgcaaatg
     3841 caaaccaact ggattttcca atccgcaaat gaagcagtga cgtgaatttg gttgttttat
     3901 ttttgacatg gtacggatta tactcggagg catgaatcag ccgaaaattt ttatcacccg
     3961 cgtcattccc agccctggct tgaatctggt cagggagcat tttctccccg tggcatgggc
     4021 tgacgaaatg ccgcccaccc gtgaacaact ccttgcgcat gtgcgcggcg tggatggtct
     4081 gctttgcctg ctcacagaaa aaatagacgg tgaactcatg gacgcggctg gtccgcagtt
     4141 gaaggttatc agcagcatgt cggtcggcgt ggaccatatt gatgtggctg ctgccacggc
     4201 gcgtggaatt cctgtgggca atacgcccgg tgtgctcacc gatgccactg ccgatcaagc
     4261 ctttgcattg atgctcgccg ccgcgcgccg cgtcaccgag gcggagcggt ttttgcgggc
     4321 aggcaagtgg gtcacatggc agccgagtct tttgcttggt gccgatctgg tcggtgctac
     4381 acttggcatt gtcggttttg gcaggatcgg tcaggcgatt gccaaacggg cgcagggatt
     4441 cgatctgcgg gttctttatc acagcccaaa tgcacagcct gcttacggcg caaaacctgt
     4501 ggacctggac acccttttgc gcgaatccga ttttgtctcg atcaatgtgc cgctcaaagc
     4561 tgagacacgg catctggtga atgcagactt tttggcgaaa atgaaaccga atgcgatttt
     4621 ggtcaacact gcccgcgggg gcgtccttga ccaaaccgcc ttgtacgatg cgctcaaatc
     4681 caaacgcatt ttcgccgccg cactcgatgt caccgacccc gaaccactgc cgatggattg
     4741 tccgcttttg gagttggaga actgcattat tgtgccgcat ctcggaagtg ccagccaaaa
     4801 aacccgcgac atgatgtcac ttcttgccgc tcagaacctg atcgccgggc tgaagggcga
     4861 acgcctgacc cattgcgtaa atccgcaggt gtatgggtag atcattttga aacactgagc
     4921 catgtggttt aaactcctgc cagaggttta atagacttca cgcaaaagcc tgaaagatta
     4981 accactgagt cacggagaca cagagaaaaa aataaaaaaa ctccgtggtc caaaatggct
     5041 tttcaaagat attccttgca acgtatgcaa gaggtcaaat aaaaaaatgt tcccagtgca
     5101 ggcaataccg ccaagggtgg caattgggcg agtgttacac cgggaaccag ttaggaaaac
     5161 atcatgctgg aagttgatct gcatcccaaa aacaccccag tcaaccctcc cgactccgat
     5221 ctggtccttt tgcgggaggt cgttaaaacc taccacggct cggcagggac attcaccgcg
     5281 ctcaaaggcg tggacttgcg cgtcgagtcg ggcgcttttg tttcgatcat tgggaaatcg
     5341 ggcagcggca aatccacgct gatcaatgtc attactggca ttgacaggcc cagttcaggt
     5401 gaagtcatcg ttgaccgtac cccggtacat accttgaacg aggagcagat cgccatctgg
     5461 cgtggtcgtt cggtcggtgt catcttccaa ttctttcaac ttctgcccac attgacggct
     5521 gtggaaaata ttttgctggc catggattat gggaagcact atccgcggct cgaacgcccg
     5581 gaacgggcca tgcaattgct tgaattggtc ggcatggcag attatgccca ccgcctgccg
     5641 ggctcgcttt ccggcgggca gcagcaatgt gtggcgatcg cacgcgcatt ggcgaacgat
     5701 cccgtcctgc tcactgccga tgagccgaca ggcaatcttg attcaaagtc cacggagatg
     5761 gtgtttcaac tttttgaaaa ccttgcaggc accggcaagg ccattctcat ggtcacgcac
     5821 gacaacgagc ttgccgtccg cgcccagcgc acgatccgcc tggtggatgg gaggattcaa
     5881 gatgaattcg tcaaccgata agccattgat cgagttgcgc tccgtggtta aggtctatga
     5941 aagcctggca ggttccgtta ctgctttgaa cggcattgac ctgcaggtct ttcagggcga
     6001 gtttttggtg gtcactggaa aatcaggaag cggcaagacc accctggtca atatgcttac
     6061 agggttggat cgctccactt caggggagat ctgggtcaat ggtgagccgt tacacaagtt
     6121 tggccctgaa aaagccgcaa ggcttcgggg caggacaatg ggtgtgatct ttcaaacgtt
     6181 tgaacttctg cctactttga ccgttttgca aaacgtgatg ctgccgatgg atttcgccag
     6241 gcgctattcc cgctgggagc agcgcaagcg tgcataccac ttactcgaac aggtggggat
     6301 cgccgatcat gcaaggaaac tcccgaccgc attatcaggc gggcagcaac aacggctggc
     6361 gattgcccgc gccatggcca atgaccctgc catccttgtt gcagatgaac ccaccggcag
     6421 ccttgactct gcaacagcga acgcagtcgt tgatatcttt gcagggttgg ttcaggaagg
     6481 cgggaccgtt tttctcgtta cgcatgacaa ggatattgcc aaattcggtt cacgggtgat
     6541 caccttgtct gatggtgaaa ttgtttaaag gagaatctcc atgctaaggg cgcgctggta
     6601 caaggttatc aacgatttat ttggcaacaa gacgcgcacg cttttgatcg tgctgtccat
     6661 gtcggttggg ctttttgcat tggggatcat tcttagcgca cgcactattc tggcggatgg
     6721 attggcggaa agttttgcaa ccatctaccc gtccagcgga accgtgcgca cgatcgagct
     6781 gttcggtgag gatttcatcg agtctgtccg caagatggat gatgtgcagg aagccgacgc
     6841 acgcaggagt gtctccgtcc gcatgcagac aaaatcaggc gagtggaaaa acctcaccat
     6901 ctttgtgttt gccgattatg acgatatccg cgtgaacaag gtcacctcac agagcggggc
     6961 ctggcccccg cccgagcgtg agattctgat cgaacgcgcg gcgctctctg tcatcgaagc
     7021 acaggttggt gacgtgatcc tgatcaggtt tccgaacgat gtcgagcgca gaattcgcat
     7081 tgcagggttg gcatatgacc ctgcccaact ccctgcgcag atcgacggca caccgtacgg
     7141 ttacatcacc tttgatacat tggaatggtt cggcgaatcc tatgggttta acgaattgca
     7201 tgtgatcgca gtaaaccctg aagacaagga ttgggcacaa caggtggtca atcgcgtcaa
     7261 ggacaaggcc gaaaattctg gctacaccat tccgttgagt atgaccgcgg agccaggtca
     7321 actcccgatg aatgatgttt tgcagggcat tcttctgttg atggggctgc ttggtgtatt
     7381 gtctctcttt ctgagcatct tccttgtggt caacaccgtg tctgctttgc ttacccagca
     7441 aaagcggcag atcggtgtga tgaaagcggt tggtggcggc acgcttcaaa ttctggggat
     7501 gtatctggtg atggtatttt cctatggtgt gctggcgttg ctgatcgcca ttccgtttgg
     7561 gatgtatggc gcacgggaac tcagttctgt tctggcggtg ttctttaatt tcgatcttta
     7621 ttcgatggat gtgccgcctc aaacatatct gatccaagcc gctgtcggat tgatcctgcc
     7681 ggtccttgcc tccctcgtgc cgttcctctc caacctgcgg atcagcgcgg cggaggcgat
     7741 gagcagctac accatgggca ggggacgctt cggtaaaaat tggattgatc aattgctttc
     7801 cggtgcaaat ttatggtttg ctcggatgtt atccatccgc tcgatcttgc tttcggtgcg
     7861 gaataccttc cgcaacaagg gcaggttgac gttaactctg attacgctca cgctggggtc
     7921 cgcaaccttt atcagtgtgt tcaacgtccg tgcttcgtta tccagcactg ttgaggatat
     7981 gatcaagtgg ttcaactgtg atacgatgtt cgctttggat cgaagttatc gtgctgataa
     8041 ggtccagcgt gaggcggaaa gcatccccgg tgtgacccaa accgatgtgt ggatccaact
     8101 cccaacgcgg atgatccgca gggatggaag tgaaagcggc atgatctaca tgttcgcccc
     8161 cactgttcgc ccggatagtt tgatcgtttc accttccctt gcggaaggca ggtggcttgc
     8221 tccaggtgac gagaatgcag tggtcgtccc ttctgcgctt ctgaacgatg aaccagaact
     8281 cgaactcggc ggcgacattg tgctgaagat tgacggtgat gaacacgtgt tcaagatcgt
     8341 gggaacattc aagggcatgg cgttccttcc gatcatgtac gtcaattatg attacatcac
     8401 gaagatcacc aaccgcatcg gccaggcaga tgcggtgatg gttgccacac aacaacatga
     8461 cgctgcctat gtggatgccg taactcgtac gctggaagaa cgttttaacc gtgtcggcgt
     8521 tgagatagga ctggcgacca ccatcaacac agagcggcgt gatgccgagg cctcttttga
     8581 ttcgatcgtt gcccttttgt tgttgatggc gatcctgctg gctttggtcg gcgggttggg
     8641 gctgatggga acaatgagta tcaatgtttt ggagcgcacc cgcgagatcg gtgtattgcg
     8701 cgccattggg gcgccaaatc gtagtgtggc gtccgttttt attctcgaag gaatcatcat
     8761 cggtttgatg agttgggcga tgggcgcggc ggtcgccatc cccatgagca ggggtttgaa
     8821 ccaggctctc ggtcaggcgg tgatgggcgt gccattgacc tattcctatt cactccccgg
     8881 gctttggttg tggttggtta tcgtcatcct tttgagtgtt ctggctagtt tcatcccggc
     8941 acgtacggct tcccgattaa cggttcgcga agtgcttgcc tacgaataac acacatgacc
     9001 gccgttgttt tgcaggcagt catatgtcca aatatgttat aggagaaaaa tgtttaacaa
     9061 gttatccctt acctttcacc gctttccacg cacgttctgg gtggtggcag ggactcattt
     9121 tattgatgtg attggcaata ctttgctcat gccgttcttt gctttatatg tcacccagaa
     9181 gtttggtgtg ggcatgaccg aggcgggtat catcctcgct acgaattcgg tggcagggtt
     9241 gatcggcagc accattggcg gtgccatggc tgaccgtttt gggcgccggg gcatcatcct
     9301 tttcggattg atcgttagtg cctttagcgg actcacgctt gggttcgtga accagttcta
     9361 tatgttttac ggcttgtcgg tatttgttgg gctgatgggc agcgctgcgc atcctgctca
     9421 caatgccatg atcgcggata tccttcccgc cgaacaaagg gctgagggct tcggcatcat
     9481 gcgcgtgatc tccaacttcg cgtggatcat cggccccacc atcggtggat ttctggctgc
     9541 ctataatttc ctttacctgt ttttgagcga tgcggtcatc agcagtatta ccgcagtgct
     9601 ggtcttccgt ttgattcccg agaccaagcc tcaacccaaa gagaaaaccg agcacgaaac
     9661 gctggcgcag accatcggcg gatatcgtgt ggcgctcttt gatcgtccgt ttctggcttt
     9721 catctttgca tccatcttga tgatcctggt gtatcagcag atgtacagtt cgctttcggt
     9781 gtatctgcgc gatgttcata acgtcaatcc gcggttttat ggattggtga tgtcttcaag
     9841 cgctgtgacg gtggtgatct tccagttttg ggtgacgagc atcatcaaga aatatccgcc
     9901 gtttttgatg atggcaaccg ctactttctt ttatatgatc ggctttggta tgtacggatt
     9961 tgtttccacc attccgttgt ttattctggc aatggtcatc attaccattg gagagatggt
    10021 cggtatgccc accagcaatg tgctggctgc atcctttgcg cccgaggaga tgcgcgcccg
    10081 ttacatggcg gtctttggcc tgacctggat ggtgccttcg atgatcggcc cctgggcggc
    10141 agggctgatc ttcgacaata tgaatccaaa ctatgtctgg tacatcagta ttgtgctgtg
    10201 tttgatcgcc atgctggcgt tttatctgtt acaagtgcgg ctgggtggac gggaacagtt
    10261 ccaacgcgca aaagacgggt aagtagaaaa tgaaaggtgg aaagtgaaat gcgggattgt
    10321 gtcttccgct ttctactttc cacctttttt atttttctgc ccagtggatt gcgatcgttc
    10381 cgaacatcaa tcttttgaaa ttgacatttt ggaaccccgc ttttgacatt ctcgacgcca
    10441 tgtcttcggc agtgacaaaa ccttctgtgg tttcaggcag atagcggtac gcttccctaa
    10501 cccctgtcag taacccaccc agggtgggga tgatgatgtg catatggatc cagatgaatg
    10561 gggagaggat atttttcttc gggcgggtgg tgtctagaat gacgattcgt ccgccatttt
    10621 tcagcacgcg atattgttcc tcgatggctt tatccagatt gatgacgttg cgcatgagaa
    10681 agcccgaaat gaccgcatcg aatgtggtgc tgttgaaggg caggtgcagc gcgtcggctg
    10741 tggaaaaatt cagagggccg ttctttttgc cgacgcgcat catttcgagg gtgaaatctg
    10801 aagcggtgat gcgggcttgt ggaaactgcg tcagggcttc gcgtgccaga tcgcctgtac
    10861 cagttcccag gtccagcaag gaagcggtgt tcgtcatccg tgtaagtttg atgaccatct
    10921 tgcgccagcg gatatcctgc ccgccagtca tcagtcgatt catcagatcg tagcgtttgg
    10981 cgatctgcgt gaacatgttt tggacgtagg cggcgcgttc gcgtcctgta agttgggtca
    11041 tgaaatctcc tgaaaataat ttgcgcaatt ataagttata ctgcccgcca tcaaacttac
    11101 tggagcaaac tttgaccgag caaacattta attggcaggc ctggggattg gcgatccgtc
    11161 cacgcacact gcctgctgcg gcggctggtg tggtgatggg cggcgcgctt gcgtggcatg
    11221 acggattttt tcggcttgat gccacgctgg tttgtttatt cgccgccctg cttttacaga
    11281 tcggcagcaa ccttgcaaac gatgtttttg attttgagcg cggtacagat acccctgaac
    11341 ggcttggccc cacgcgtgtg actcaagctg gcttgctgac atcgtcacag gtgaagttgg
    11401 gaatggcggt tgtctttgta ctttcggcgt tgatggggct ttaccttatc tggcttggcg
    11461 gttgggtcat ccttgccatt ggtattgctg cgatcatttc cgccatcgct tacacaggcg
    11521 ggccgtttcc gcttggttac tacgggttgg gcgacgtttt tgtctttatc ttttttggtc
    11581 ttgcttcggt ggcagggact tactacgtgc aggctggcga tgtttcttct gctgcgtggt
    11641 ggatgacact tccaccaggg ctgatcatca ctgcgatttt ggtggtcaat aatctgcgcg
    11701 accttgaaaa tgaccgcaag gctggcaagt atacgctggc ggtgatgttc ggcgaacaat
    11761 tcgcaaaggc tgaatatttg acctgtatct ttgcggctta tttgattttg cctgttgcgg
    11821 cgtggtttgg gttgattccg tggttgtctt taatggcatg ggcttcgttt ccaattgcct
    11881 tccgcgccat tcgcacggtc tttacccaaa aaggacgtcc gcttaacgct gcgcttgcgg
    11941 gtacagggca ggcggcattt atgtttagtt tgttgttttg gattgggttg gcgctgcaga
    12001 ggtagaaaag aaagaagtaa gaagaaagaa gattgatttt tcaa
//
LOCUS       DAOQLC010000125        11942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2052_length_11942_cov_22.419282, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000125 DAOQLC010000000
VERSION     DAOQLC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11942
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2052_length_11942_cov_22.419282"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..370
                     /locus_tag="PL000_17360"
     CDS             119..370
                     /locus_tag="PL000_17360"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNC90701.1"
                     /translation="MQITISFSEKPFPAEPKEGVARKFRLYAALGMYRAGELSMGAAC
                     ELAGVDRYVFTDFCKTQGVVIQTQTPDELESDFSRLAGK"
     gene            375..848
                     /locus_tag="PL000_17365"
     CDS             375..848
                     /locus_tag="PL000_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90702.1"
                     /translation="MLVVADSSALVALSVCEALDLLLKIYGDVRVPRAVYDEVVVIGK
                     SQSAALQSFLEGRVVEVDLSRWVLAAGGLGQGELEAMALYRQLSADVLLIDDRRARAV
                     AEHNQIVCLGALGFLLLAKQNGQVRLVAPYIERLRSSSLYYGADLLEKFLLLAGE"
     gene            1466..3337
                     /locus_tag="PL000_17370"
     CDS             1466..3337
                     /locus_tag="PL000_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90703.1"
                     /translation="MSVVFKKMFFACVVAVMVLTTGAWQNSQDVLAASPRQSAPEAPL
                     FSSLTWADLGSVSRSITTSVRGDAITLSGKGYAAAERFQGGVSQDVADYYSNAQLAAS
                     GWTSYDAYDTAEGTHKVFYHDAGYYFAVDFLTCADDSSSTCVTVWMSEKTAPSPAAQF
                     TGFAPDPFATTFSKTSPASGTINLDPKNLTLTWGTYSPTPDKYSYCVKIGSACDDNDP
                     NWTGTYLNTSVKLTNLEYDKIYYWQIKAITCATCIPKKFVYANDAKWWTFTTKPQSAA
                     GVVILGNVGTPSATLTYVVSGTTKTVSSDGSGGYSITVPSGWTGTITPSKVGFYFTPI
                     NASFSNLTAAQTIQNFTATPTGSGTPEISGNAGVAGAILSYSDGGQAKTAIADSTGAY
                     KFTVSANWTGTVTPSKDNFYFNPAYRSYTNVTAHQTGQNYTISIFYDVPSTYWAFNYI
                     ERLYAAGITGGCSSSPLMYCPEGTVTRAEMSVFLLRGIHGSAYVPPAVGSSTGFNDVP
                     ASHWAAAWIKQFATEGITGGCGTSLYCPEGKASNAEMAVFLLRSKHGAAYTPPAVGGS
                     TGFSDVPTSYWAAAWIKQLAAEGISTGCGNGKFCPDDAVTRASMAVLMVKTFSLPKQ"
     gene            3566..4033
                     /locus_tag="PL000_17375"
     CDS             3566..4033
                     /locus_tag="PL000_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90704.1"
                     /translation="MPRLKKYLIIACLLIVTLFNQSCATPAAPTAAFTPVIVMTEEPS
                     LEMPTTTPSAPSLTIDPAFQRGQVIPLSVGDVFAISVPPGSSIWKVAYAAEVVEPLTP
                     ADKMTEPGSQGWLFRAVAAGQTDIRLTAPAPACAKPPCPPAAPMVFVFTVDVK"
     gene            4193..5452
                     /locus_tag="PL000_17380"
     CDS             4193..5452
                     /locus_tag="PL000_17380"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNC90705.1"
                     /translation="MRSRLALLFLLVIFLFTTACGQQGPRPVSFMVFGEPAELKAYQD
                     LVAAFEKQHPEIKVELIHIPSQGDYRKRLAADLVGGEPADVVLINYRRFSGLALKNAF
                     EPLGPYLEKSESIAYDDFYALSMRAFSLNDQVVCIPQNISSLVAYYNKDLFDAAGIPH
                     PDAGWTWDEFISASKALTLDVDSDGRVDQYGAGVEPTLIRVAPFVWQNGGKVSINNAL
                     TLAEPLDLEAVQWFVDWQVTHHIVPNAEEEKAESSESRFINGRVAIYFNSRRLVPTFR
                     EITAFDWDVAPLPVGRSDATILHSDAYCLTAASQHKEDAWTFIEFANSAAGQSLIAQS
                     GRTVPSLIQVAESEAFLDPSAKPEHSRVFLDVIPFTLRLPQQTNWADIEEICDEELQR
                     AFFGEVTALEAMQSAVERALPLFTENE"
     gene            5445..6341
                     /locus_tag="PL000_17385"
     CDS             5445..6341
                     /locus_tag="PL000_17385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC90706.1"
                     /translation="MNRTYTASSRTQLLLMLTPYLLGAVVLVGLPALLSLLLAFLRYD
                     GISAPVWVGGFNFQLVLANPVDLLFPISLYNTLYYVILAVPVRVLSALALALLLNRRG
                     KGVGLYRAVVYLPTIIPDVAYALIWMWIFNPLYGPLNLILEGLGLPTPGWLADAQYAK
                     MVFVVMTAFQIGEGFVVLLAGLQNIPPEYYAAAAVDGGNFWQQFRYITLPLLAPWLLL
                     LTLRDVILSSQNIFTANFIMTNGDPYYSTLFLPLLIYEEAFDRMHFGPGSAMTVLLGL
                     VTAALIGFILSIVGSWGETDAI"
     gene            6331..7176
                     /locus_tag="PL000_17390"
     CDS             6331..7176
                     /locus_tag="PL000_17390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC90707.1"
                     /translation="MQFNSQRRMFHSIAFHAVSILISALFILPLVWMASASLRQPGLP
                     PPRTVEWWPEPFAWSNYTRIFEMLPMGRYVLNSLLVAGLAVPITLVTASLAGFAMSQV
                     NRRWRENLLGLSVVLLVVPTVAVWVARFILFRTVGLFNSYLALLAPSVMGSSPLFVLL
                     FFWSFRRIPRELYDAARMDGAGPLEIWWRVALPLSNATMIVVGILTFLLYWGDFTTPL
                     FYLKSQTLYTLPVGLRQLQELDRTNWPLLLAGSMVMTLPSVLLFGIVQRFFLPENRLA
                     GIYGQ"
     gene            7272..>7972
                     /locus_tag="PL000_17395"
     CDS             7272..>7972
                     /locus_tag="PL000_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90708.1"
                     /translation="MTKRNRMFLSIASSLMLLAVALGLWGRLGPMSTQVTQALSSNVQ
                     HVETDEKEADHPEEAREYRALAWRDENGNIPADGLFTAAQQINEMRAQQGNNPQAITS
                     AGWTWIGPGNIGGRVRSIIIHPTDTNIMWAGSVTGGIWKSTDGGASWNILTDFMSNMA
                     VTTMVIDPSNPNILYAGTGEGFTGNREISEPLGGGVFKTTDGGSTWTQLSSTNNSNWY
                     FVNRLAIHPSNPQIL"
     assembly_gap    7973..8072
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8073..9953
                     /locus_tag="PL000_17400"
     CDS             <8073..9953
                     /locus_tag="PL000_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90709.1"
                     /translation="ALVNSGNDFLSGQGWYDNALWVDPTNPNTLVVGGVDLYRSTNGG
                     STLTQISEWWNSPQSAHADHHVIVEKPGFNGTSNTTVYFGNDGGVYRANNVYTVVGTT
                     GWTELNNNLGITQFYGAAGNATTGKIIGGTQDNGTLIYTPAGGTEGWTTTFGGDGGWS
                     AADPTDDNYLYGEYIYAQIHRSVNGGVYSDPIWGEYWDGSSYQYKNPPYIITDTYNSN
                     ANFISPFILDPNDSNRMLVGAASLWRSNDVKAPNTTTTGPSWYVIKSSTGYNITAIAV
                     APGNSDIVWVGHENGDVFKTTNGTATNPTWTRADTGSPALPNRYVTRITIDKNDNNKA
                     YVTFGGFSTDNVWATTNGGSTWADITGSGATGLPSLPVRSLVIHPNDSNWLYIGTELG
                     LFASSDGGATWGLPHDGPTNTPVDELFWMGTKLVAATYGRGLFTQEASNTLFADVPAD
                     YWALSFIERLYNAGITGGCGTNPLIYCPEAPVTRAQMAIFILRGIHGSAYVPPAATGT
                     VFTDVPAGSFAADWIEQLAAEGITGGCGNGSYCPDAYITRAQMAIFLLRGEHGSAYTP
                     PAATGTVFGDVPVGAFAADWIEQLAAEGVTSGCGGGNYCPDANVTRAEMAVFLVRTFS
                     LP"
     gene            10126..>11942
                     /locus_tag="PL000_17405"
     CDS             10126..>11942
                     /locus_tag="PL000_17405"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HNC90710.1"
                     /translation="MKSFSILFRYLLIFMLVAGTFTNVPVASAIPSAASGPALSVNAA
                     ADVHPISPYIYGMNFATEAIAADLNLPVRRWGGNSTTRYNWQLDVHNTGADWYYENIP
                     EDNAHPELLPNGSESDEFVEQDRRTNTETIMTVPLIGWTPKARREDHPYDCAFKVSKY
                     GPQDSTDYWDPDCGNGWHNGVQLTGNNPTDTSVAITSSFVTAWINHLKGNYGTAANGG
                     VMFYNLDNEPMLWNSTHYDVHPNPVTYDEMRDKTYAYAAAIKAADSSAQTLGPVEWGW
                     CAYFYSAADGCSPGADRAAHGNVDFVPWYLQQMKAYEQAHGVRILDYLDLHVYPQVDG
                     VYSDALGSAAVQSARLRSTRQLWDPGYVHEGWIGQPVYLIPRMEDWVANNYPGTKLAI
                     TEYNWGALGYMNGALAQADILGIFGREGVDLATLWGTPDDFNAPGIFAFRMYRNYDGA
                     GGTFGDTSVQSSSADQSQLAIYAAKRSTSGTLTLMVINKTASTITSDLTLSGFQPVDT
                     AQVYRYSSANLGAIVKQADQAVTSSGFTASYPANSITLIVIPSSVSQTTFVDVPADHP
                     LYAYIQALYDAGYTAGCSTDPMMYCPDTILDRAQSAVFML"
BASE COUNT         2586 a         3183 c         3087 g         2986 t
ORIGIN      
        1 tccgcgtatg gattcacggg cgagatggcg gatgtcagcg ggttgacgta tctgcgggcg
       61 cgggatgttc tacgtatcaa attcgagtat catagggcag gtagagcaag gagctaaaat
      121 gcaaatcaca atttcattct ctgaaaaacc ttttcccgcc gaacccaaag aaggtgttgc
      181 acgtaaattc cgcttgtacg cggcgctggg aatgtaccgc gcgggggaat tgtccatggg
      241 cgcggcgtgt gagctggcag gtgtggaccg atatgtcttt accgattttt gcaaaacaca
      301 aggtgtggtt atccagaccc agaccccgga tgagttagag tcagattttt cgcggttggc
      361 aggtaagtaa aaccatgctt gttgttgcag atagttccgc attggtggct ttgtcagttt
      421 gtgaagcgtt ggatcttttg ctcaaaatat atggtgatgt ccgagttcct cgggcggtat
      481 atgatgaagt ggtagtgata ggcaaatcac aatctgcggc tttacaatct tttcttgaag
      541 ggcgagtggt ggaagtggat ttatctcgtt gggtgctggc ggcaggcgga ctgggacaag
      601 gggaattgga agcaatggct ctttatagac aactctcagc cgatgttttg ttaattgatg
      661 atcgtcgtgc aagggcagtg gctgaacata atcaaattgt ttgccttggt gcattggggt
      721 ttctgttgtt ggcgaaacaa aatggacagg ttcggcttgt cgctccatat atcgaaagat
      781 tgcgttcgtc ttccttgtat tatggtgcag acctattgga aaaattttta ttgcttgctg
      841 gggagtagtg cccaatcctg cgccccgtat gggacagtac gtgacacctc gcacttgacc
      901 cctgaaaccc tcaccactta tagattcacg ggcgagatgg cggatgtcac ttttgcaaaa
      961 actggacctc ttgcagcaaa ccgactatgg agaatcccaa aactccatct gccctcacct
     1021 gtttccccta cacatgccac aagagatcca tttcataata ttacctgcca ggctgtaatg
     1081 atgtcacccc tctaattgga ggggtgtcat tttcaaattt gcaagacagg aaacagggat
     1141 tgccacccac ataggcagct aacaaaaaaa ctttgaacca ctgctcctgg aaaacgacaa
     1201 caaaaaacgc catagatttt cgtgtagaca atggaatccg cgcctgaatc cctgtaagta
     1261 tgcaaacgaa aaacgtacga ttcccctacg cgcggaacat tttccttttt tcgtccgttt
     1321 aatatggtgt acaatactta agattaaatg ggtacccgtt ttaatttaac gggcccgctg
     1381 gctggtttaa caggttgctc aaatcatcat aactgctctg atgacccgaa agcccagccg
     1441 aagcaccaaa aacaaggaga taaaaatgtc cgtagtcttc aagaaaatgt tttttgcctg
     1501 tgttgtggcc gtaatggttt tgaccactgg cgcatggcag aactcacagg acgtgcttgc
     1561 tgcatccccc cgccagagcg cgcctgaagc tccactattt tccagcctca cttgggccga
     1621 tctcggctct gtcagtcgca gcattaccac cagcgtcaga ggcgatgcga tcaccctctc
     1681 tggtaagggc tatgccgcag cagaacgatt ccagggcggc gtttcgcagg atgtagctga
     1741 ttactattcc aatgcccaac ttgccgcttc tggctggacc tcctacgatg cctatgacac
     1801 cgcagaaggc acccacaagg tcttctacca cgatgcaggc tactatttcg ccgtcgattt
     1861 cctcacctgt gccgatgact cctccagcac ttgtgtcacc gtgtggatga gtgaaaaaac
     1921 ggctccttct cccgccgccc aattcactgg ttttgccccc gatccatttg ccaccacctt
     1981 cagcaaaacc tctcccgcca gcggcaccat caacctcgac cccaagaacc tcaccctcac
     2041 atggggcacc tacagcccca cccccgataa atacagttac tgtgtcaaga tcggctcggc
     2101 ttgtgacgat aacgacccga actggaccgg tacctacctc aacaccagcg taaaactcac
     2161 caacctcgaa tacgacaaga tctactactg gcagatcaag gccattacct gcgccacctg
     2221 tattccgaag aaattcgtct acgctaacga cgccaaatgg tggaccttta ccaccaaacc
     2281 gcagagcgcc gcaggcgtgg tcattttggg taacgttggc acccccagcg ccaccctcac
     2341 ctatgtagtc agcggcacca ccaagaccgt ctcatccgat ggcagcggcg gatactcgat
     2401 caccgtccca agtggatgga ccggcacgat cacaccctcc aaagtcggat tttatttcac
     2461 cccgatcaac gcttctttca gcaacctgac agctgctcaa acgatccaaa actttaccgc
     2521 caccccaaca gggtccggta cacctgagat cagcggcaat gcaggtgtgg caggcgccat
     2581 cttaagctac tccgacggcg gacaagccaa gaccgccatt gccgatagta ccggtgccta
     2641 caaattcacc gtctccgcaa actggaccgg taccgtcaca ccctccaaag ataatttcta
     2701 tttcaacccg gcctatcgca gttatacgaa cgtcactgcc catcagaccg gccagaacta
     2761 caccatctcg atcttctatg atgtgcccag cacctattgg gcgttcaact acatcgaacg
     2821 tctgtacgcg gctggcatca ccggcggctg ttcctccagc ccgttgatgt actgccccga
     2881 aggcaccgtc acccgcgctg aaatgtcggt cttcctgctg cgcggtattc acggctcggc
     2941 ctatgttcct cctgcggttg gcagcagcac aggtttcaac gatgttcccg cttcacattg
     3001 ggccgccgca tggatcaagc aattcgccac cgaaggcatt accggcggat gcggtaccag
     3061 cctgtactgc ccggaaggca aagcctccaa tgctgaaatg gccgtcttcc ttctgcgttc
     3121 caagcatggt gccgcttaca ccccgcccgc ggtgggtggc agcaccgggt ttagcgatgt
     3181 cccaacttct tattgggccg ccgcatggat caagcagttg gccgccgaag gcatctccac
     3241 tggttgtggc aatggaaaat tctgccccga tgatgccgta acccgtgcca gcatggcggt
     3301 cttgatggtg aagaccttca gcctgccgaa acaataagct ttctgagttt tgaatccaaa
     3361 accctcgcga aaatcttcgc gggggttttt tgtacccgta ccgcagactt tagtctgcca
     3421 tttaccattc aaagcggact gaagcccaca ttacgagcct tacaaaagag gaagaaaatc
     3481 acctcggttt ttattgccta atcataaaaa aaattgtata gtgtgggagt aatgattcca
     3541 accaatgtga ttggaaatat catccatgcc tcgtttgaaa aaatatctta tcatcgcctg
     3601 tttattgatt gtgacccttt tcaatcagtc gtgtgccacc cctgccgcgc caacagccgc
     3661 tttcactcca gtcattgtga tgacggagga accctccttg gaaatgccca ccacaactcc
     3721 atctgctcca agcctgacga ttgaccctgc cttccaacgc ggacaggtga ttccgctttc
     3781 ggtgggggat gtgtttgcaa tttccgtgcc gccaggttcc tcgatttgga aagtggcata
     3841 tgcagccgag gtggttgagc ctctgacacc cgcagataaa atgacggagc ctggttcgca
     3901 agggtggttg tttcgtgcag tggccgccgg gcaaactgat atccgtttga ctgcacctgc
     3961 gcctgcctgt gccaagccgc cttgtccacc ggctgccccg atggttttcg ttttcaccgt
     4021 cgatgtgaag tagggagtct atttttaggg tggcgaaatg cagattggac agaatcaggt
     4081 ttgaggtcgg ttggaggcgc agtttggttt caagattctt gttcagggaa gacatccgat
     4141 taaattgatc ttcttctttc taaaaaatcc cgtcgttgaa acaggagaaa atatgcgaag
     4201 tcggttggcg cttttgtttt tattggtgat ctttcttttt acaaccgcat gtgggcagca
     4261 gggacctcgc cccgtcagtt ttatggtgtt tggtgaacct gccgagttga aagcgtacca
     4321 ggatttggtg gcggcttttg aaaagcagca ccccgaaatt aaagttgaat tgatccacat
     4381 tcccagccag ggtgattacc gcaaacggct ggcggcggat ctggtaggcg gtgagcccgc
     4441 cgatgtggtc ttgatcaatt atcggcgttt cagcgggctg gcgctcaaga atgcttttga
     4501 gccgctggga ccgtacctcg aaaaatcaga gtcgatcgcg tatgatgatt tttatgcgct
     4561 ttcgatgcgt gcgttttcgc tgaacgatca ggtggtttgc attccgcaaa atatttcaag
     4621 cctggtggcg tattacaaca aggatttgtt tgatgcagcg gggattcccc accccgatgc
     4681 aggctggact tgggatgaat ttatttccgc ttcaaaagcg ttgactttgg atgttgattc
     4741 ggatggccgc gtggatcaat acggcgcagg tgtggagccg accttgattc gtgtggcgcc
     4801 ttttgtttgg cagaatggcg gcaaggtcag catcaacaat gcgctcacac ttgccgagcc
     4861 gcttgatctg gaagccgtgc aatggttcgt agattggcag gtgacgcatc acattgtgcc
     4921 gaatgccgag gaggaaaaag cggagagcag tgagagtcgt tttatcaacg ggcgtgttgc
     4981 catctatttc aacagccgcc gtttggtgcc gaccttccgc gagatcacgg cttttgattg
     5041 ggatgtggct cccctgcctg ttggaagaag cgatgccacc attcttcatt ccgatgccta
     5101 ctgcctgact gcggccagcc agcacaagga agatgcatgg accttcatcg aatttgccaa
     5161 ctccgccgct ggacaatcgc tgatcgcgca aagcgggcgc actgtacctt cgctgattca
     5221 agtggctgag tcagaggcct ttctcgaccc aagtgcaaaa cctgagcaca gccgcgtatt
     5281 tctggatgtg attcccttca ccctgcgttt gccgcaacaa accaattggg ccgatatcga
     5341 agaaatttgc gacgaggagt tacaacgcgc attctttgga gaggtcacag ccttggaggc
     5401 catgcaatcg gcggtggagc gtgccctgcc cttgttcacg gaaaatgaat agaacttata
     5461 ccgcttcatc ccgcacgcaa ttgcttttga tgctgacgcc ttacttattg ggggcggtgg
     5521 tgttggtcgg cttgcccgcc ttgctttcat tactgctggc gttcctgcgt tacgatggaa
     5581 tttccgcgcc tgtgtgggtg ggtggtttta attttcaatt ggtgctggca aatcctgttg
     5641 atttgctttt tcccatttcg ctgtacaaca ctttgtatta tgtgatcctg gctgtgcctg
     5701 tgagagtgtt gagcgccctg gcattggcct tattgctcaa tcgccgcggc aaaggtgtgg
     5761 ggttgtatcg ggcggtggtg tacctgccca ccattattcc cgatgtggcc tatgccttga
     5821 tctggatgtg gatattcaac ccgttgtatg gtccgttgaa cttgatcctt gaagggttgg
     5881 gcctgcccac acccgggtgg ctggccgatg cccaatatgc aaaaatggtt tttgtggtga
     5941 tgaccgcatt tcagatcggc gaaggctttg tggtgttgct ggcagggttg caaaatattc
     6001 cgcctgaata ttatgccgcc gccgccgtgg atggcggtaa tttctggcag caattccgct
     6061 acatcaccct gccgctgctg gcaccgtggc tgcttttact caccctgcgc gatgtcattt
     6121 tgagcagcca gaatattttt accgccaatt tcatcatgac caatggcgac ccgtattact
     6181 ccactttgtt tttgccgctg ctgatctacg aagaagcctt tgaccgaatg cactttggcc
     6241 cgggctccgc catgaccgtg ctgttgggct tggtcaccgc cgcattgatc gggttcattc
     6301 tttccattgt gggcagttgg ggagagaccg atgcaattta attcccaacg caggatgttt
     6361 cactcgatcg catttcatgc cgtctcgatc ttgatctcgg ccctgttcat tctgccgctc
     6421 gtttggatgg cttccgcttc gcttcgtcaa ccaggcctgc ctccgccacg aacagtggag
     6481 tggtggcccg agccttttgc atggtcgaat tacacgcgta ttttcgagat gctcccaatg
     6541 gggcggtatg ttcttaattc cctgctcgtt gcaggccttg ccgtgccgat cacactggtc
     6601 actgcttcgc tggctggctt tgcaatgtct caggtgaatc gccgctggcg ggaaaatctt
     6661 ctcgggttgt ctgttgtcct tttggttgta cccactgtcg ctgtgtgggt ggcgcgcttt
     6721 attttgttcc gcactgtggg gttgtttaat tcatatctgg ctttgctggc tccgtcggtc
     6781 atggggtcga gtccgctgtt tgtgctgttg ttcttttgga gttttcggcg cattccccgt
     6841 gaactgtacg atgccgcccg gatggatggt gccggtccac tggaaatttg gtggcgggtg
     6901 gctttgccgc tttcaaacgc cacaatgatc gtagtgggca ttctgacctt tttgttgtat
     6961 tggggcgatt tcaccacgcc cttgttctat ttgaaatcgc agactcttta caccctgcca
     7021 gtgggtctgc gtcagttgca ggaattggac cgcacgaact ggccgttgct tttggcaggt
     7081 tcaatggtga tgaccctgcc ctctgtgttg ttgtttggaa tcgtccagcg gttctttttg
     7141 ccagaaaacc gccttgccgg tatttatgga cagtgagttt gaaaaaggag gtgccttgta
     7201 cctgagtaac tgaacttgcg ttttgaggaa tagttttttc attgaaccca attattacaa
     7261 ggagaatgat catgacgaaa agaaatcgaa tgttcctttc catcgcttca agcctcatgc
     7321 ttttggccgt tgcgcttggt ctgtggggca ggcttggccc aatgagcact caggtgaccc
     7381 aggcattgtc ttcgaacgtg caacatgtgg agaccgatga gaaggaagcg gaccatcctg
     7441 aagaggcccg cgagtatcgt gctttggcct ggcgcgacga gaacggcaat atccctgcgg
     7501 atggcttgtt tacggctgcc cagcaaatca atgaaatgcg cgcccagcag ggaaacaatc
     7561 cgcaggcgat caccagcgca ggctggacgt ggatcggccc gggcaacatc ggcggacgtg
     7621 tgcgctccat cattatccac cccaccgata cgaatatcat gtgggctggc agtgtgacgg
     7681 gcggcatttg gaaatccacc gatggcggcg cttcatggaa catcctgact gattttatgt
     7741 ccaacatggc tgtgacgacc atggtcattg acccgtccaa cccgaatatt ttgtacgctg
     7801 gtacaggtga aggtttcaca ggcaaccgtg aaatttccga gcctctcggc ggcggggtgt
     7861 tcaaaaccac ggatggcggc agcacatgga cacaactgtc ctccaccaat aattcaaact
     7921 ggtattttgt gaatcggttg gcaattcatc cgtccaaccc gcagattctg ttnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcactcgt caactccggc aatgacttcc
     8101 tctccgggca gggttggtac gacaatgccc tttgggtgga tccgaccaat cccaacactc
     8161 ttgttgtggg cggcgtggac ctttaccgaa gtacgaacgg cggctcgacc ctgacccaga
     8221 tcagtgaatg gtggaattca ccgcaatcgg cccatgccga ccatcatgtc attgtggaaa
     8281 agccaggttt caatggcaca agcaacacaa ccgtttactt tggtaacgat ggcggcgtgt
     8341 accgcgccaa taatgtgtac acagtcgtcg gcaccactgg ctggacggaa ctgaataaca
     8401 acctcggcat cacccaattc tacggcgcgg caggcaatgc cacgacgggc aagatcatcg
     8461 gtggtacgca ggataacggc accctgatct acactcccgc aggcggcaca gaaggttgga
     8521 ccacaacctt tggcggtgat ggcggctggt cggcggccga cccgacagat gataactatc
     8581 tgtatggcga atatatctat gcacagatcc atcgcagtgt gaatggcggc gtgtactcag
     8641 acccgatctg gggtgaatat tgggatggct cttcctacca atacaagaat ccgccctaca
     8701 tcatcaccga tacctataac tcgaatgcca atttcatttc gcccttcatc ctcgacccga
     8761 acgattcgaa ccgcatgctg gttggtgcag cttcgttgtg gcgttccaat gatgtcaagg
     8821 cgccgaacac caccacaaca ggcccatcct ggtatgtgat caaatcatcc acgggctaca
     8881 atatcactgc cattgcggtt gctccgggta attctgatat cgtctgggta gggcatgaaa
     8941 atggcgatgt gttcaagacc accaacggca ccgccaccaa tcccacatgg actcgtgcgg
     9001 atacaggttc tcctgccctg ccgaaccgtt atgtcacccg cattaccatt gataagaacg
     9061 acaataacaa ggcctacgtc acgtttggtg gtttctccac tgataatgtg tgggccacga
     9121 ccaatggcgg ctccacctgg gctgatatca ctggcagcgg cgcgacgggt ttgcccagct
     9181 tgccggtccg cagccttgtg atccacccga atgattccaa ctggctgtat atcggcacag
     9241 aactcggtct tttcgccagt tcagatggcg gcgccacttg gggtctcccg catgatggcc
     9301 cgaccaacac ccccgtggac gaactgttct ggatgggaac aaaactggtt gcagcgacat
     9361 acggacgcgg cttgttcaca caggaagcct ccaacacgct ctttgccgat gttcccgcgg
     9421 attattgggc cttgtcgttc attgagcggt tgtacaatgc cggcattacc ggtggctgtg
     9481 gtaccaatcc gctgatctat tgcccggaag cgcctgtcac ccgcgctcaa atggccatct
     9541 tcattttgcg tggtattcat ggcagcgcct atgttccacc agcagcgacc ggtacggtct
     9601 ttaccgatgt ccctgccggg tcctttgcag cagactggat cgaacagctt gcggctgaag
     9661 gcatcacggg tggttgtggc aatggaagct actgtcccga tgcctacatc acccgcgccc
     9721 aaatggcgat cttcctgttg agaggcgagc atggcagtgc ctacactccg ccagcggcta
     9781 cgggtacggt ctttggtgat gtccccgtcg gcgcatttgc tgcagattgg atcgagcagc
     9841 ttgcggctga aggtgtgacg agcggctgtg gcggcggtaa ctattgcccc gatgccaatg
     9901 tcacccgcgc tgaaatggcc gtcttcctgg tacggacgtt cagtttgcca taatcaatac
     9961 cccatattcc agtaagcaga cgcaccccga aaacaatttc ggggtgcgtt ttctatttct
    10021 caagccgttg cctcgatggt accgatgtat gtttcaaaat tttaagtgga ggggtacact
    10081 atatctgtca aatcccttcc cagaaaaagt gcataggaga ctcgaatgaa aagtttctca
    10141 atcctatttc gttatttatt gatttttatg ctggtggcgg gcacgtttac caatgtgcct
    10201 gttgcttcgg caattccctc agcggcctca ggcccggcat tgagtgtgaa tgctgcggct
    10261 gatgtacacc ccatcagccc atatatctat ggaatgaatt ttgccacgga agccattgcc
    10321 gctgacttga atctgccagt ccgccgttgg ggtgggaatt cgaccacacg ttacaactgg
    10381 caattggatg tccacaacac tggcgcggat tggtattacg agaacattcc cgaagataat
    10441 gctcacccgg agttgctgcc caatggttct gaatcagacg agttcgttga acaggatcgc
    10501 cgcaccaaca cagagacgat catgaccgtt cccctcatcg gctggacgcc caaggcccgc
    10561 agggaagatc atccgtatga ttgcgcattc aaagtcagta aatatggtcc gcaggacagc
    10621 acagattatt gggaccctga ttgcggcaat ggctggcaca acggtgttca actcacaggc
    10681 aataacccca ccgataccag tgtagcgatc acttcttctt tcgtcactgc atggatcaat
    10741 catttgaagg ggaattatgg cacggctgcc aatggcggcg tgatgttcta caatctggat
    10801 aacgagccga tgctgtggaa cagcactcat tatgatgtgc atcccaaccc ggtcacctac
    10861 gatgaaatgc gcgataaaac ttatgcgtat gccgctgcga tcaaagccgc cgattcatcc
    10921 gcgcaaacgc ttggaccagt ggagtggggc tggtgcgctt atttttattc tgccgctgat
    10981 ggttgttccc ccggtgcaga ccgcgccgcg cacggcaatg tggattttgt gccttggtac
    11041 ctccagcaga tgaaagccta tgaacaagcc catggcgtgc gcatccttga ttatcttgac
    11101 ctgcatgtgt atccgcaggt ggatggtgtt tattccgatg cgttgggcag tgccgctgtg
    11161 caatcggcgc gtttgcgttc cacccgccaa ctttgggacc caggttatgt gcatgaaggc
    11221 tggatcggcc agcctgtgta tttaattccg cgcatggaag attgggtggc gaacaattac
    11281 cccggcacga aactcgccat cactgaatac aactggggtg cgctcggata catgaacggc
    11341 gcactggctc aggctgatat cctcggcatc tttggccgcg aaggcgtgga tctggcaact
    11401 ttgtggggca cgcccgatga tttcaatgca cccggtattt ttgcatttcg catgtatcgt
    11461 aattacgatg gcgcgggcgg cacgtttgga gataccagcg tgcaatcttc cagtgccgac
    11521 caatcgcaac tggcgatcta tgcggcgaag cgcagcacga gcggcacatt gaccttgatg
    11581 gtcattaaca agaccgcctc aaccatcacc agcgacctga ccttgtcagg atttcaaccc
    11641 gtggatactg cacaggtcta tcgctatagt tctgccaatt taggagccat tgtcaaacaa
    11701 gcggatcagg cggtcacctc cagcggattc acagccagct accctgcgaa ttcgatcacg
    11761 ttgatcgtga tcccttcctc tgtttcacaa accacttttg tggatgtccc cgcggatcat
    11821 cctttgtatg cctacattca ggcgctgtac gatgcaggct acaccgcagg ctgctccacc
    11881 gacccgatga tgtactgccc cgatacgatc ctggatcgcg cgcaatctgc cgtgttcatg
    11941 ct
//
LOCUS       DAOQLC010000126        11902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2063_length_11902_cov_13.769815, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000126 DAOQLC010000000
VERSION     DAOQLC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2063_length_11902_cov_13.769815"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..654)
                     /locus_tag="PL000_17410"
     CDS             complement(19..654)
                     /locus_tag="PL000_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90711.1"
                     /translation="MGKHGNNDWLRGFFAEQDVFIIGSGKSADSLTTKQINKLQKSKT
                     IVMGNRAKQFKAPSIFLFLDSDLIERLDMPGAWPYGESFHVLAGPQSRMVEEANVHRF
                     GQVHDIVSQNPSYLLCDHSGLAAVNAAFIGRARRVFLVGVDCGFGVWKASGTPPTLQD
                     PDFKLMRRYMSTVPLFEKLHKKMQNLYRTTDDTNLKFLPFMDLDVALAGQK"
     gene            complement(638..1009)
                     /locus_tag="PL000_17415"
     CDS             complement(638..1009)
                     /locus_tag="PL000_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90712.1"
                     /translation="MDVAASRIEKCRGEIVRVSKENRTEKIYKKFTDDLADSAKKLER
                     HLGVLRGMRGNALWDFQQRVMLDGENKKTNAQITVGDRMFALIQQDPKYKAYKKPRRS
                     GSGMLMGRIGNLKVVLNGKAR"
     gene            complement(1035..1670)
                     /locus_tag="PL000_17420"
     CDS             complement(1035..1670)
                     /locus_tag="PL000_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90713.1"
                     /translation="MKGCWKKEEAAYVLGDGPSLRDFDYSLLIGKNVIACNDAYFRLQ
                     EIGLNPSIWMFVDAKFVGQCKSRRGINFYDLPFPIVAGQHCGLRERDNVRLFISAREP
                     TMAEGSLYYREHTGAAAINMAIQTGAKNIYLLGIDLDYIGDLSHSRPGHRMDGSENRS
                     SYQKQIKRYERFASFASNITNLSTISALQSFPKKSWQEHFGVSDAKPKSDI"
     gene            complement(1671..3128)
                     /locus_tag="PL000_17425"
     CDS             complement(1671..3128)
                     /locus_tag="PL000_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90714.1"
                     /translation="MSWATQLLSRTGGYLADVTSAFRLKTDLAKHGDAPAEVGSVLIV
                     SENDDGSVTGTPYLKSPETSSDFRLRTGIDSILFQDNFNATAQNTQIWKYVSSTLTAA
                     MSGGFLQINSAAAGNTGTYVSMQTFQYFPLVKTAPLYIDVDFILSAAPIPANERFRIG
                     LFVPNTTNLPIDGCYAVLSSVGLQIVMNFNGTETSTTLAGFTPSANTNYHLCLVVGVN
                     EVEVWIDDVLMGEVDVPAGNSQPFMQGALPLSVECVNTGAVTGAMTLKIGSMQVSIAD
                     LAFGFSVPEVQALAGLGGGHGQNGQTQGQSTQWANAAEPTGAAATNTTAALGTGLGGL
                     FKANAMATSATDIIISSFLNPANTINITGRNLVIRGVWVDVMNQVTAVATTAFGFAMA
                     LAYGHTALSLATAETGSFATGTTHAPRKVPIGFLYLPVAAPVGQAAGKQYVEFAAPII
                     VRPGEYVAIVAKVIVGTATASETLLFLCGFNAYFQ"
     gene            complement(3125..>3433)
                     /locus_tag="PL000_17430"
     CDS             complement(3125..>3433)
                     /locus_tag="PL000_17430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90715.1"
                     /translation="PAGADGADAVVHEGTAEIDFGSTPTDSGSVSITGQTGILTTSRP
                     RAWFVSRATTDNSAADHEQAAARCVVICSEPIADTGFTISVFCQGYVTGKFSIEWSWI
                     "
     assembly_gap    3434..3533
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3534..3772)
                     /locus_tag="PL000_17435"
     CDS             complement(<3534..3772)
                     /locus_tag="PL000_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90716.1"
                     /translation="MPPEEIIVIEKEIIVIEKEIETVTVLSETIAGPQGLPGGSILYG
                     SGAPNNSLGNAGDSYIDTDVGDLYAKDQIGWNLELS"
     gene            complement(3772..4137)
                     /locus_tag="PL000_17440"
     CDS             complement(3772..4137)
                     /locus_tag="PL000_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90717.1"
                     /translation="MAATKNFTISAGATFEFTLLYKDSAGVAIDITGWLFQMKAKRTF
                     SQNETPALSIDETDGILVTGAEGRLDVTISAARTDAIRDAWIATSKIDGYYELLGIDP
                     GGKAKILLSGKLTVIPGVI"
     gene            complement(4137..4577)
                     /locus_tag="PL000_17445"
     CDS             complement(4137..4577)
                     /locus_tag="PL000_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90718.1"
                     /translation="MNIRAKLKTAIINEIANSTSVDSAHISKFRDRPVDFSEMPFVVV
                     SSMDEPADRLDDLHTYKMTSQILITVYTGGVADPEEQSDLICGEIEDALLNPLCELID
                     TDTGDAIGKSLTLIGTKVRRQERAEKTAVFAAMLFECEAYAEIS"
     gene            complement(4574..4942)
                     /locus_tag="PL000_17450"
     CDS             complement(4574..4942)
                     /locus_tag="PL000_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90719.1"
                     /translation="MSDFFLDGMAADNAAIHSLDAEFTESATIEHPLVDPTISQEIPV
                     VFSETYQTLNPETGIPAMSSSPGIMIYLPPVDVLIGTSLLSLIPSGCRIIIRGISYLL
                     SEPQPDGMGCYFVKLKREIV"
     gene            complement(4948..6021)
                     /locus_tag="PL000_17455"
     CDS             complement(4948..6021)
                     /locus_tag="PL000_17455"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNC90720.1"
                     /translation="MSGVLDNLFETRSMLPALMQMRAPTAFLRNLFFKQEKTFDTEAV
                     DIDIYKEAQRRAAHVRLYQEGTLMERQDYSTYTYKPFPLKPIMITTAREAFRRGPGEA
                     LYNSVSPMARAQATLARDLLTMVDAIGRTEELMCSQALFNDAVKTKDVRGNDLAADIS
                     FGRAADHNVSSAGVTSGYGGLWDASGSDPLETLRKLKRKVAKDSGFVPTVAVFAGDIT
                     DMFLSHSVIRDMAKDTRSSQIDIQMRFPTYLTDGAVYVGRFEGLDVFEYMGTIRDDYT
                     DPDNPTVVDLVPAKKILLGNPDARSEMLYGAVDDLDSLAPVARFPKSWRENNPSAQFV
                     QVISRPLPAPHQVDSYIVAQVDA"
     gene            complement(6035..6400)
                     /locus_tag="PL000_17460"
     CDS             complement(6035..6400)
                     /locus_tag="PL000_17460"
                     /inference="COORDINATES: protein motif:HMM:NF014921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HNC90721.1"
                     /translation="MSLLDTSNYDNLIAGQDPDPTTRNITVKSGENLVRGQVAAIETS
                     TGKVVAYDSSESDGHNAARFIVFEDVDATSGDTKGTVYTTGRFNESALVFTTTGELDD
                     TVREALRDYGIIVSPSIQN"
     gene            complement(6402..7619)
                     /locus_tag="PL000_17465"
     CDS             complement(6402..7619)
                     /locus_tag="PL000_17465"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNC90722.1"
                     /translation="MSYRYFAIKPEALETLQVIMSEKKERRADMMPGQSVPQKTVHAV
                     VGSIGLLNISGALWKSEDDAMWYGGTSYEMLNSALTQLESDPAVRAIVMMMDSPGGEV
                     SGTGEFAARIAASQKTVVACVEGMACSAAYWIASQSDFLVMSETSEVGSIGVVATYMD
                     AKGFFESKGIKIYQVVSSQSPMKRPDLASDEGMAEVRKTIDALAEIFVGKVSEGRNVS
                     RETVLSDFGRGGVLVGIDAIKRKMADGVMGLREALGLMASGPAPSGPVAVENVAADVQ
                     PVAPAVAVVEPAPAAPVVAQDPKSSFDAGVKAERERIQAIEDLEIKGYDALIAEAKFK
                     TGVDAASVLLAVNAEEKKRRAALQSNIIADAAELPADMRSVGSETASEIDDVTEGLIQ
                     AARAVSGKSAGGN"
     gene            complement(7616..9238)
                     /locus_tag="PL000_17470"
     CDS             complement(7616..9238)
                     /locus_tag="PL000_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNC90723.1"
                     /translation="MARRTKLQIAKEKLELIETQQKIQKYELQQKVMAIVDTQVGHGG
                     GTPYDGASITSQANQGWYAFSGDPDDTLLGMQRLRERSRDLYTNNVVGGGAIRTKTSS
                     VIGSGLKLQSAVDPKLVGLADTVVREKENEIERLWEMWVKEADASNQCDYYELQDQAY
                     RTFLICGDSFTVFPRIERPNSPFSFRIKVIEGDRCSNPNYQSDNAALRGGVVIGPNEE
                     PLGYHFRFRKALWQDTWQYFPAFGGKSGRRNAIHLMRQDRPGAKRGTPMLTIMMSHLK
                     QIGRYMDAETMAAVISSFFVGFVESSNDDALSSTITNFFQREAGIPDATPPRYDIVPG
                     TIQHLRNGEKMNLSAPGRPNNSFGAFMQSMMEFAGMGLEIPYEILMRHYSSSYSASRG
                     ARIDWERVAKIDRVWFNRKWNQQIYENWLFEQVAFGMLDLPGFLTDPYVREAWCNAVW
                     TGDAAGLLDPQKEVSAAISRIEAGLSTRTLEAAGLTGMDFEHILTILATEQQMMKDKG
                     VTIGMPSQSSGSGFQKNQDPQNSDQQPQGEGQ"
     gene            complement(9250..9489)
                     /locus_tag="PL000_17475"
     CDS             complement(9250..9489)
                     /locus_tag="PL000_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90724.1"
                     /translation="MASFTLAQAQENLERATQAYQNALSGKGYTRMNGGSSMTVNRQE
                     ILTLKSEMIYWQNMVNDLSGVTSNRQKVAIPRDIR"
     gene            complement(9527..>9883)
                     /locus_tag="PL000_17480"
     CDS             complement(9527..>9883)
                     /locus_tag="PL000_17480"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNC90725.1"
                     /translation="DVWFKQLTAESLVQRNKKGGGVDFSWVLPPGKRAEALDCRVYAR
                     AAAAGLSVDRFTDVQWDRIREEFNSTIKGDRRIQKQQKRDAPAPPRMSGAPSGMQLKP
                     SHRPARGLRADWHDPD"
     assembly_gap    9884..9983
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9984..11197)
                     /locus_tag="PL000_17485"
     CDS             complement(<9984..11197)
                     /locus_tag="PL000_17485"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HNC90726.1"
                     /translation="MAKSSKPRSAPKKTKASIELNTSADDLIFGVGVASLKPEAKFPV
                     SEWADAFRVLSPVSSAEAGPWRTSRTPYAREIMDMLSSGSPAQRVIFMKASQIGGTEI
                     GLNWLGYIADMAPGPTLYVVPNESFAKDYSRLRITPMIDESERLKSKISEAKSRDSGN
                     TIMRKEFPGGYLAMTGANVPSNLRGKPIRYLFMDELDEFPGDVGGQGDPVELAITRTR
                     TFSRRKKIYAASSPTIAGRSRIEKLFEESDKRYFFVPCTSCGHMQRLVWSQIKWVDRE
                     PNTAVYECEKCQHHMREFEKTSMLANGEWRATQTAKNAATVGYHLSALYSPPGMFSWA
                     ECVESFLKSKEDRLLLKVFVNTILGEVFEERGDAPDWEILSARKENYPRGLIPSGGYI
                     ITAGVDIQKDRA"
     gene            complement(11175..11744)
                     /locus_tag="PL000_17490"
     CDS             complement(11175..11744)
                     /locus_tag="PL000_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90727.1"
                     /translation="MGEKITQAEFARRVGVAKKNINKAIKSGRIQKDADGKIDFETAA
                     RDWKENRDPAMDRGSQSQSSDESSSGYARARTLQTAWNVKIKQLQWEKAAGKLIEKEA
                     VVASLRKFTIYVRDSILAIPDRVAARMSAQVEEYIARVLRKHLPKNVAEKVIEEISSD
                     QIHKITRDTWEAESRAVMEELENGKVIKT"
BASE COUNT         2779 a         3186 c         2742 g         2995 t
ORIGIN      
        1 catcggattc ttaatcgttt atttttgtcc agcaagcgca acatcaaggt ccatgaaagg
       61 gagaaatttt aggttcgtgt catcggtcgt gcgatacaga ttctgcattt tcttgtgcaa
      121 cttttcaaac aaaggaaccg tgctcatgta ccttcgcatc aatttgaaat ccggatcttg
      181 cagtgtgggt ggagtgccgc tcgctttcca tacaccgaaa ccacagtcga cgcctacgag
      241 gaacactctg cgcgcgcgcc cgatgaatgc agcgtttaca gcggccagac cggagtgatc
      301 acaaagcaag taagaaggat tctgcgaaac tatgtcatgc acctgaccga atcggtgaac
      361 gttcgcctcc tcaaccattc tggattgagg gcccgcgaga acgtgaaacg attcaccgta
      421 aggccatgca ccaggcatgt ctaggcgctc aatcaaatcg ctatcgagaa aaaggaagat
      481 cgacggagcc ttaaactgct tcgcgcgatt tcccataacg atcgtttttg atttttggag
      541 tttgttgatt tgctttgtcg ttaaagaatc cgcagacttc ccagatccga tgataaaaac
      601 atcctgctcc gcgaagaaac ctcgcagcca gtcattatta ccgtgctttc ccattcaata
      661 ccacctttag atttccaatc ctgcccatga gcatcccgga cccgctacgc cttggctttt
      721 tgtatgcttt gtattttgga tcctgctgaa tcaacgcaaa catccggtcg cccaccgtga
      781 tctgagcatt ggttttttta ttttctccgt caagcataac tctctgctga aaatcccaga
      841 gtgcatttcc acgcatgccg cgcaaaacac cgagatgacg ctccagtttt tttgcagagt
      901 ccgcaaggtc atccgtgaat ttcttataga tcttttcggt tcggttttct tttgaaacgc
      961 ggactatctc accgcgacat ttctcaatgc gactcgcggc cacatccata gattcgacta
     1021 cagcctgcac tttttcaaat atctgacttc ggcttagcat cacttactcc aaaatgttcc
     1081 tgccatgatt tttttgggaa cgattgaaga gcagaaattg tactaaggtt tgtgatgttt
     1141 gaagcaaagg aagcgaaccg ctcatatcgc ttgatttgtt tttggtaact agacctattt
     1201 tctgacccat ccattcgatg ccctggacgg gaatggctca ggtctccaat gtagtctaga
     1261 tctattccga gcaaatagat gttttttgca ccggtctgga tcgccatgtt tatggcggca
     1321 gcgccagtgt gctcgcggta atacaggcta ccttccgcca ttgttggctc gcgcgcggaa
     1381 atgaataaac gaacgttgtc cctttcgcgc aatccgcagt gctgacctgc aacaattgga
     1441 aaaggcagat cgtaaaaatt aattccgcga cgcgacttgc attgccctac gaattttgcg
     1501 tccacaaaca tccaaatgga tggattcaag ccgatttctt gaaggcgaaa atacgcatcg
     1561 ttgcatgcga taacgttctt tccgataagt agagaataat cgaagtcgcg caggctggga
     1621 ccatctccca gcacatacgc ggcctcttct tttttccagc aacctttcat ttactgaaag
     1681 taagcgttaa agccacaaag gaacagaagc gtctcggatg cggttgcggt tccgacaatt
     1741 acctttgcga cgatcgccac gtattcacca ggacgaacga taatcggagc ggcaaactca
     1801 acgtactgct tccctgccgc ctgaccaact ggagcagcta caggaagata gagaaaccca
     1861 atcggaacct tcctcggcgc gtgcgtcgtt ccagttgcga atgacccggt ttctgcggta
     1921 gcgagcgaaa gcgctgtgtg cccatatgca agagccatcg cgaaaccaaa tgcagtggtg
     1981 gcaaccgccg tcacttgatt catcacatcc acccatacac cacgaatcac tagatttcgt
     2041 cccgtgatat tgatcgtgtt tgcaggattc aaaaaggatg atatgataat atccgtcgcg
     2101 cttgtcgcca tcgcgtttgc tttgaacaaa ccgccaaggc cagtgccgag tgccgccgtt
     2161 gtgtttgtag cggcagcgcc agtaggttct gcagcattcg cccattgagt ggactggccc
     2221 tgagtctgac cgttctggcc atgacctcca ccaagtcccg cgagtgcctg aacctctggt
     2281 acgctaaaac caaacgcaag atccgcgatc gaaacctgca tagatccgat tttcagagtc
     2341 atcgcaccgg tgacggctcc tgtgtttacg cactcaactg aaagcggaag cgctccctgc
     2401 atgaaaggct gagagttacc tgcaggaaca tctacctcgc ccatgagaac atcgtcgatc
     2461 caaacctcta cctcattcac gccgacaaca agacacaaat gatagtttgt gttcgcggac
     2521 ggagtaaatc cagcaagagt ggtggacgtt tccgttccat tgaagttcat aacgatctgc
     2581 agaccaacgc tagagagaac cgcatagcat ccatcaatcg gcaagtttgt agtgttcgga
     2641 acaaacaagc caatgcggaa tctttcgttc gcaggaatcg gtgcggcaga aagaatgaaa
     2701 tcaacgtcga tatacagcgg cgcagttttc acaagtggga aatactgaaa cgtttgcatt
     2761 gatacgtacg ttcccgtatt acctgcagcc gcagagttta tctgcaagaa accgccagac
     2821 atagcggcgg tcaaagttga tgaaacgtat ttccagattt gagtattctg cgcggtagca
     2881 ttgaaattgt cctgaaagag aatcgaatca attccggtgc gcagacgaaa atcagatgaa
     2941 gtctctggac ttttcaggta cggcgtcccg gtgactgacc cgtcatcatt ttctgaaacg
     3001 atcaaaacag atccaacctc tgctggcgcg tctccgtgct tcgcaagatc agtttttaaa
     3061 cggaacgcgc tagtgacgtc ggcaaggtag ccgccagtgc gtgaaagtaa ttgtgtagcc
     3121 cagctcatat ccagctccat tcgattgaaa atttaccagt cacatagccc tggcagaaaa
     3181 cagaaatagt aaaaccggta tccgcgatcg gttctgaaca aatcacaacg catcgagcag
     3241 cggcctgctc atgatccgca gcggaattat ccgtagtcgc gcgcgaaaca aaccatgctc
     3301 gaggacgcga agtcgtgagt attccagtct gaccggtgat ggatacgctt ccagaatcag
     3361 tgggagtaga tccaaaatca atctccgcag ttccctcatg cacaaccgca tcagcaccat
     3421 ccgctcctgc gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngaaagtt
     3541 caagattcca accgatctgg tccttcgcgt acagatctcc aacatcggta tcgatgtaag
     3601 aatccccagc attccccagt gaattattcg gagcgccgga gccatacaaa atagatccgc
     3661 caggcaatcc ttgtgggccc gcaattgttt ctgaaagaac ggtgaccgtc tcaatttctt
     3721 tttcgatgac tatgatttct ttttcgatga ctatgatttc ttcgggaggc attagatcac
     3781 ccccggaatt actgtgagct tgccggacaa aagaatcttc gcctttcctc ccggatcgat
     3841 tccaagcaac tcgtaatacc cgtcgatctt tgaagtggcg atccaggcat ctcgaattgc
     3901 atccgtgcgt gctgcgctaa tcgttacatc aagccttcct tctgctccgg tcactaagat
     3961 cccatctgtc tcatcaattg aaagagccgg ggtttcattc tgcgaaaacg tacgcttcgc
     4021 tttcatctgg aaaagccatc cggtaatatc aatcgctact ccagctgagt ccttatacaa
     4081 aagcgtgaat tcgaatgtcg cgccggcaga gattgtgaag ttctttgttg cagccattat
     4141 gaaatctccg cgtaagcctc acattcaaac aacatcgcag caaagactgc cgtcttctcc
     4201 gcacgctcct gccggcgcac ctttgtgccg atcagagtga gggattttcc gattgcatcc
     4261 cctgtgtccg tgtcgatcaa ttcgcaaagg ggattcaaaa gagcgtcctc gatttcgcca
     4321 cagatcagat cgctttgctc ctctggatct gcaacaccgc cagtataaac agtgataaga
     4381 atctgactgg tcattttgta agtgtgcaaa tcgtccaggc gatctgccgg ttcatccatt
     4441 gatgatacaa cgacaaaagg catctcagag aaatcaaccg ggcggtcccg aaattttgaa
     4501 atgtgcgcag agtcaacact ggtcgaattt gcaatttcgt ttatgattgc tgttttcagt
     4561 tttgcgcgaa tgttcatacg atttctctct tgagcttcac aaagtagcac cccatcccat
     4621 caggctgtgg ctcagaaagc aaataagaaa ttccacggat gatgatcctg cacccggacg
     4681 ggataagaga gagaagagaa gtgccgatca aaacgtccac tggcggcaga tagatcatga
     4741 tgccaggact tgagctcatc gccgggatgc cggtttctgg attcagtgtt tggtaagttt
     4801 cagaaaatac tacagggatt tcttgtgaga tggttgggtc aacgagtgga tgctcgatgg
     4861 ttgcggactc ggtaaactca gcatcgagtg agtgaatggc cgcattgtct gcggccattc
     4921 cgtccaagaa aaaatcgctc attcagatca ggcatcaacc tgtgctacaa tatacgaatc
     4981 cacttgatgt ggcgctggaa gtgggcgaga aatgacctga acgaattgcg cgctcgggtt
     5041 attttctctc catgatttag ggaagcgtgc gactggagcc agggaatcca ggtcgtctac
     5101 cgcgccgtaa agcatctctg agcgtgcgtc tggattccca aggagaatct ttttcgcagg
     5161 caccagatca actacggtcg ggttatccgg gtccgtgtaa tcgtcgcgaa tcgtgcccat
     5221 gtattcaaac acatccagac cttcaaagcg cccaacatac acagcaccat cagtgaggta
     5281 tgtggggaat cgcatttgaa tatcgatctg gctggatctg gtatccttcg ccatgtcgcg
     5341 gatcacagaa tgcgaaagga acatatcggt aatgtcccct gcgaatactg caaccgttgg
     5401 aacaaaaccg gaatctttcg ccacctttct cttcagcttg cgcagagttt caagcggatc
     5461 tgagccggat gcatcccaca gtccaccgta accggaggtt actcctgcgg agcttacatt
     5521 gtgatccgca gcgcggccga aagaaatgtc tgcagcaaga tcattgccgc gtacgtcttt
     5581 cgtttttacc gcatcattga aaagtgcctg agaacacatc agctcctcgg ttcttccaat
     5641 tgcgtctacc atagtgagca gatcgcgtgc gagtgtcgcc tgcgctcttg ccatagggga
     5701 aacggaattg taaagagcct cgccaggacc acggcgaaac gcttcgcgcg cagtggtaat
     5761 catgatgggc ttcagaggga acggcttgta ggtgtaagtc gaatagtcct gacgttccat
     5821 cagggttcct tcttggtaca gcctcacgtg cgccgctctg cgctgtgctt ctttgtagat
     5881 gtcgatgtct acggcttcgg tatcaaaagt cttttcttgc ttgaagaaaa ggtttctgag
     5941 gaaagcggtc ggcgctctca tttgcatgag agcaggaagc atcgacctcg tttcgaatag
     6001 attgtctaat acgccgctca ttgtgttttt ctccttagtt ttggatggat gggctcacga
     6061 taattccgta atcgcgaagc gcctcacgta cagtgtcatc cagctcgccg gttgtggtga
     6121 acaccagagc ggattcgtta aagcgaccgg tcgtatagac cgtgcctttt gtatcaccac
     6181 tggtggcgtc tacgtcctca aagacgatga atcgcgcagc attgtggccg tctgattcag
     6241 atgagtcgta agcaaccact tttccagtgg aagtttcgat cgccgctacc tgaccacgaa
     6301 ccaagttctc gccggacttg accgtgatat tcctggtagt ggggtccgga tcttggccgg
     6361 cgatcagatt gtcatagttc gatgtgtcca atagagacat atcagtttcc tcctgcagat
     6421 ttgccgctta cggctcgcgc cgcttgaatc aggccctcgg ttacatcgtc gatttcagaa
     6481 gcggtttctg atccgaccga tctcatgtct gccggcaatt ctgccgcatc agcaatgata
     6541 ttgctttgga gcgcagcacg gcgttttttt tcttcagcgt tcacagccag aagaacggac
     6601 gccgcatcga ctccggtttt gaatttcgct tccgcgatca gcgcatcgta tcccttgatt
     6661 tccagatcct caatcgcctg gattctctca cgctcagctt tcacaccggc atcaaaggat
     6721 gattttggat cctgcgctac aactggagcc gccggtgcag gttccactac cgctactgcc
     6781 ggcgcgacag gctgaacatc tgccgcaaca ttttcaactg ccaccggacc cgatggtgca
     6841 ggaccggaag ccatgaggcc aagagcctcg cgcaaaccca tcacaccatc ggccatcttt
     6901 cgtttgattg catcaattcc cacgagaaca cctccgcgtc caaagtcaga cagcacagtc
     6961 tcccgtgaga cgttcctgcc ttcgctgacc ttccccacaa aaatttctgc tagagcgtca
     7021 atcgtttttc ttacttccgc catcccttca tcggacgcaa ggtccgggcg tttcattggg
     7081 gactgcgaag aaacaacctg atagatcttg attcctttgc tttcaaagaa tcccttagca
     7141 tccatgtagg tggctacaac gccgatcgac ccgacctcag acgtctcgct cattacaaga
     7201 aaatcagatt gtgacgcaat ccagtatgcc gcagaacatg ccatgccctc cacgcatgca
     7261 accaccgttt tctgtgatgc cgcaatgcgc gccgcgaatt ccccagtgcc agaaacctct
     7321 ccgccaggag aatccatcat catcacaatc gcacgcacgg caggatcgga ttcaagctga
     7381 gtgagcgcag agttgagcat ttcgtaagaa gtgcctccat accacatagc atcgtcctcc
     7441 gatttccata gtgcacctga gatattgaga aggccgatcg atccgactac cgcatgcaca
     7501 gttttctgcg gaacagactg accaggcatc atatcagcac gcctttcttt cttctccgac
     7561 attatcacct gcagggtttc cagcgcttct ggtttgattg cgaaatatct gtagctcatt
     7621 gaccttctcc ttgtggttgc tgatcagaat tctgcggatc ttgatttttc tggaaaccag
     7681 atccggatga ttgcgaaggc atgccgatcg tcacaccttt gtccttcatc atttgttgtt
     7741 cggttgcaag gattgtgagg atgtgctcaa aatccattcc ggtcaatccg gcagcttcaa
     7801 gcgtacgagt agagagccct gcctcgattc tggaaattgc agcgctcacc tctttttgtg
     7861 gatcgagcaa gccagccgcg tctccagtcc acaccgcatt acaccatgct tcgcgcacgt
     7921 aaggatcggt caggaatcct ggcagatcaa gcatcccgaa cgcaacctgc tcaaacaacc
     7981 agttctcgta gatctgctga ttccattttc ggttgaacca gacacggtcg atttttgcga
     8041 cacgctccca gtcaatgcgc gcgccacgcg aagcggagta ggatgatgag tagtgccgca
     8101 tgagaatttc gtaggggatc tccaatccca tgccggcaaa ttccatcata ctttgcatga
     8161 acgcgccaaa cgaattgttt ggacggcccg gagctgacag gttcatcttc tcgccattac
     8221 gcaaatgctg aattgtccct ggcacaatat cgtatcttgg aggagttgca tccggaatcc
     8281 cggcctcacg ctgaaagaag tttgtgatcg tagagctcaa agcgtcatcg tttgacgact
     8341 cgacaaagcc aacgaagaac gaggaaatca cagcggccat agtctccgca tccatatatc
     8401 tcccgatctg cttcagatgg ctcatcataa ttgtgagcat tggagtccca cgcttcgcac
     8461 ctggacgatc ctgccgcatc aagtgaatgg cattccttcg tccgctttta ccgccgaacg
     8521 caggaaaata ctgccatgtg tcttgccata gtgccttgcg aaatctgaaa tgatagccca
     8581 gaggttcttc attcggacca attacaacgc caccgcgcaa cgccgcattg tccgattgat
     8641 agtttggatt tgaacaacga tctccctcaa tgactttgat gcggaaagaa aatggcgaat
     8701 tggggcgctc gattcttgga aaaactgtaa acgaatcacc gcaaatcaaa aaggtgcgat
     8761 acgcttgatc ctgcaactca tagtaatcgc attggtttga tgcgtccgct tccttgaccc
     8821 acatctccca cagccgctcg atttcgtttt ccttctcgcg tactacagtg tctgccaggc
     8881 caaccagctt tggatctaca gccgactgca acttcaagcc ggacccgatg acgctggatg
     8941 ttttcgtgcg gatcgctcct ccgcctacaa cattgtttgt gtacagatcc ctgctgcgct
     9001 cgcgaagcct ctgcatccca agcagagtat catccggatc tccgctaaaa gcataccatc
     9061 cctgatttgc ctggcttgtg attgatgcgc catcatacgg agtgccgcca ccgtgaccga
     9121 cttgcgtgtc tacgattgcc atcaccttct gctgaagctc atatttctgg atcttttgct
     9181 gagtttcaat cagctccagt ttttcttttg cgatttgaag tttcgtgcgt cgagccatct
     9241 gaaatctcct taacgaatgt cgcgcgggat cgcgaccttt tgcctattgc ttgtgactcc
     9301 gctaagatca ttcaccatgt tctgccagta gatcatctcc gatttcagcg taagaatctc
     9361 ctgacgattc accgtcatgg acgatcctcc gttcattcgc gtgtagcctt tgccggacaa
     9421 agcgttctgg tatgcttgag tggcgcgctc gagattttct tgtgcctgcg ctaacgtgaa
     9481 agatgccatg caccccccac tcacgggctg gacgcttggt caatccttaa tcaggatcat
     9541 gccaatcggc acgcaggccg cgtgctggtc ggtgcgatgg cttcaactgc atcccacttg
     9601 gagcgcccga cattctgggc ggagccggcg catctcgctt ttgttgtttt tggatcctgc
     9661 gatcgccctt gattgtggaa ttaaattctt cccggatgcg atcccactga acatcagtga
     9721 atcgatcaac gctcaatccg gcagcggcag cacgcgcata gactcggcag tccagggcct
     9781 ccgctctttt cccaggagga agcacccagc taaagtcgac gcctcccccc ttcttgtttc
     9841 tttgcaccaa agattctgcg gtgagttgct tgaaccagac atcnnnnnnn nnnnnnnnnn
     9901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnntcagcac ggtccttctg aatatccacg ccggcagtga
    10021 tgatatatcc tccggacgga atcaatccac gcggatagtt ttcctttcgc gcggaaagaa
    10081 tctcccagtc cggagcgtct ccacgttcct cgaaaacctc gccgagaatc gtgttcacaa
    10141 aaacctttag aagcagacgg tcctcttttg atttcaggaa cgactcaacg cattcagccc
    10201 atgagaacat acccggtgga gagtaaagcg cagagagatg atagccaacg gtagctgcgt
    10261 tcttagcagt ctgagtcgcg cgccattctc cgtttgcaag catcgatgtt ttttcaaact
    10321 cccgcatatg gtgctggcac ttctcgcatt catagaccgc agtgtttggt tcgcgatcca
    10381 cccatttgat ctgtgaccat accagccttt gcatgtgacc gcatgaagtg catggaacaa
    10441 agaagtatcg cttatcagat tcctcgaaaa gtttctcaat gcgcgaacgg ccagcgatcg
    10501 tcggcgatga tgcagcgtag attttcttcc ggcgcgagaa agttctggta cgagtgattg
    10561 caagttctac cggatcgccc tgcccgccaa catcacctgg aaattcgtcc agctcatcca
    10621 tgaacaaata tctgatcggc ttaccgcgaa ggttgctcgg cacgttcgct ccggtcattg
    10681 caaggtatcc tccggggaat tccttccgca tgattgtgtt tccagagtcg cgtgacttcg
    10741 cctcactgat ttttgatttg agcctttcgc tttcgtcgat cattggagtg atacgcaggc
    10801 gtgaataatc ttttgcaaaa gattcattag gaacaacata cagagtcggg cccggtgcca
    10861 tgtcggcaat atagcctaac cagtttaaac cgatctcggt cccaccgatc tgcgatgcct
    10921 tcatgaagat cactcgctgt gccggagatc cggatgaaag catgtccatg atttcgcgcg
    10981 cataaggagt gcgactggtt ctccacggtc cagcttccgc acttgagact ggagagagaa
    11041 cacgaaacgc atcagcccac tcggagacag gaaacttcgc ttctggtttc agagatgcaa
    11101 caccgactcc gaaaatcaaa tcatcggctg acgtattgag ctcgatgctg gccttcgttt
    11161 tctttggagc gcttctaggt tttgatgact ttgccattct ccagctcctc catcaccgct
    11221 ctagattcag cctcccaagt atctcttgtg attttgtgga tctggtccga tgaaatctcc
    11281 tcgataactt tctcggctac atttttcgga aggtgctttc tgagaacgcg tgcgatatac
    11341 tcctcaacct gcgccgacat gcgagccgca actcggtcag ggattgcaag aatcgaatcg
    11401 cggacataga ttgtaaattt tctcaggctt gcaactaccg cttctttctc aatcagcttt
    11461 cccgcagcct tctcccattg gagctgcttg atttttacgt tccacgcggt ttgcaaagtc
    11521 cgcgcgcgtg catatccgga cgatgactca tctgaggatt gcgattgcga tcctcgatcc
    11581 attgctggat ctcgattctc tttccagtct cgagccgcag tttcgaaatc aattttcccg
    11641 tcggcatcct tctgaatgcg tccgctcttg atcgccttgt ttatattctt cttcgcaacg
    11701 ccaactcgcc tggcgaattc cgcctgagtt atcttttcgc ccatcagcta gtctttcctt
    11761 ccgcattcgt ttgataagcc tatacggatc aatcccagct ttgcaaagac acagaccgcg
    11821 cacaatctca gccgcagtga actcatcggt tacccccggc ttttttttca ctgactagca
    11881 aggccccgcg ctgccccaaa cc
//
LOCUS       DAOQLC010000127        11772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2098_length_11772_cov_25.625331, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000127 DAOQLC010000000
VERSION     DAOQLC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2098_length_11772_cov_25.625331"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PL000_17495"
     CDS             complement(<1..1382)
                     /locus_tag="PL000_17495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNC90728.1"
                     /translation="MPTPESILKSIFGYDTFKPLQREIIANVQARRDTLVVMPTGGGK
                     SLTYQVPALMFDGLTVVVSPLIALMKDQVEQLNALGVPAVFLNSSLSQEEYQENMDSV
                     KSGQSKLLYVAPETLLTPRIYSLLSSLKLDLLAIDEAHCISEWGHDFRPEYRQLVDVR
                     RKFPSAVCMALTATATPRVRADIAASLGFTQSNEFLASFNRENLFIEVSPKREPIAQT
                     LRFLENFKDQSGIIYCFSRKQVDDLTATLARHQYSVRSYHAGLEDDERRRNQEAFIRD
                     DVQIIVATIAFGMGINKPNVRFVIHFDLPKSIESYYQEIGRAGRDGLPAHCLLLYNYG
                     DASKIRYFIDQKEEPERSASYQHLEAMTRYAEGSICRRKPLLSYFGETFAQENCGTCD
                     NCNNEPVDQQDITVAVQKFLSCVKRSGERFGAAHVVDILLGSKNEKIERYKHHELSTY
                     GIGRELTKSQ"
     assembly_gap    1484..1583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1651..4851
                     /locus_tag="PL000_17500"
     CDS             1651..4851
                     /locus_tag="PL000_17500"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC90729.1"
                     /translation="MASIPISKTKIIPPRRRDELLTRKRLVDFLFDSLDKKLILVSAP
                     AGYGKTSLLIDFANQSDIACCWLALDEMDRDPQRFLAYLIAAIAERFPDFGAASVSML
                     GGLTSFEQDMEKMLVMLVNEAYEQIHEHFILVLDDFHLIENIQPIQSFLNRFLQLVDE
                     NCHVVISSRVLNSLPDLPLMVARDQVSGLGFADLAFHPDEIQSLLAQNNNLHISDDEA
                     RKMIETTEGWIAGLQFSGMGFLPPSHTGVGLSDFLGQQVLDRQPEYLRDFILRTSMLG
                     EFDSSLCETVLAPLYAEPQNWHRLIASVVQNNLFVLPVGDDGRWLRYHHLFRDFLRKR
                     FEQTHPEEVRPILQRLGQAYENLAEWEKAYYTYKQLNDTGLQAALIEHAAATMLLNAR
                     VTLESWLNDLPPSMLRTRPGLLSIRGTILDTKGQAREGLDLLNQAEQAFRAEQNVPGL
                     ARALLRRGSVHRFLGDFDATLRDAEESMQLTEAVDDLQVMYAEGLRLKALALYRLGHA
                     RQSVSFFESSLNLYIRLKHTPTIPILFMETGMAYQTIGSYAEAGTAYDKALQIWQRDG
                     NLLWQASLLNNLAILHQSYGEYEKAALAFENGLLCARRSSYTRMEALISIGLGDMYSE
                     LEDFSIALTNYENAEEIARRINDRFLIFYTITARVRLALLQKDADGAHRIMEQDVELF
                     QSNASSRYERGLLALMRGHLALLTHENAEAVAHLEEALRCFSEDGRKLEGDTSRIWLA
                     AASHQNRNNELAAQVLKPLLGTQPAHPVLVAVHQAQMWLNGLQTHPEVGRMVGDLFIR
                     AARFSARIPADRRQLRRMAHVLQIPNPHMVIQAFGRAAVSVGGKTLSLSDWQTQSVRD
                     LFFYFLTASRAFTKEQIAETLWPEVDEPHKIRLRFKNEIYRLRRAVGQEIIAYEDIFY
                     KFNRSLDFEYDVEAFEAFLAHAKAAATPVEQIGYYKKAVDLVQGPFLEDVYADWATPE
                     RERLSQVYLSTLLTLGDLLLKQTQTEQALAICKRALDYDVTFEAAYALSMQVYHRMGD
                     RAAIVRTFQTCEEVMQRELGLPPSRETKELYKRLVM"
     assembly_gap    4908..5007
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5326..5469
                     /locus_tag="PL000_17505"
     CDS             5326..5469
                     /locus_tag="PL000_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90730.1"
                     /translation="MKQQAASIGIFLQRIDRQQIQLILAIASLVALALGSGAPLDGGS
                     GGG"
     gene            5470..6036
                     /locus_tag="PL000_17510"
     CDS             5470..6036
                     /locus_tag="PL000_17510"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HNC90731.1"
                     /translation="MILILFVAVGLLVGLLWAWFLKTPYHGPDLKAMWLALLGFLPQI
                     LVARLPNLLPNRMDAASLTISQVALLGFAWLNRHVAGMYLFICGAALNLLVMGANGGF
                     MPVSPQTADRLFSEEALLELSPGDRIGPKDILLLPQQTRLEWLADRFLPPAWFPFQAA
                     FSFGDVFIAVGVFWMLVRPNTLSGNRGT"
     gene            6038..6316
                     /locus_tag="PL000_17515"
     CDS             6038..6316
                     /locus_tag="PL000_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90732.1"
                     /translation="MLAPITIRRPASVNITAARPGVSRGLSRVFSAAVVNKQFCELLL
                     KDPQKALSRGYLGESFLLSKEEFDLILSIRASSLSDLARQVNRYQNGN"
     gene            6409..7911
                     /locus_tag="PL000_17520"
     CDS             6409..7911
                     /locus_tag="PL000_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC90733.1"
                     /translation="MALLKKMITELSLQNLPDASRQLWAVFQAFDDQLFVLDADGVIL
                     DFKLGGQVSHLPVSMDMLLMRKIQNIFPAEIGAKLADVLNKIRTGSRVSHLEYSLQTP
                     SGRLWFESRLVALANGQIIMMVRDITRHKISEETIKRQFGQLAALRSIDMAITSDLGL
                     KPVLSMILEQTRMQLGVDAASILLFDPQTNLLEFAADAGFFTKALQHTRLLVGEELAG
                     RAIQDQSVVYVDNLLEQPTAFQRSPQFRLENFISYYAVPLISRGQPVGVLEIFHRARL
                     ETDQEWLNYMETLAGQAAIAIDNGTLFKHLQQSNAELMLAYDKVIEGWSRALDLRNKE
                     TEEHSRRVTELTVRLAERMGIVQGELTHIHRGAMLHDIGKVAIPDSILLKPGPLTDEE
                     WTVMRSHPLIAVELLAPIEHLVPALDIPRSHHEKWNGEGYPDQLSGWGIPLSARIFSV
                     VDVFDALISDRPYRPGWPVGKALQYIKEQSEIHFDPHVVPIFIDMMESRV"
     gene            complement(8033..10282)
                     /locus_tag="PL000_17525"
     CDS             complement(8033..10282)
                     /locus_tag="PL000_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90734.1"
                     /translation="MNKNNLQIGEKIETSLSALILIFLIFFSYAFLFRMPYAGFYFNP
                     SEGRVDMVYVKTTPETSLQIGDILIKVGDITLDEFRKNRRLSFFENTRPEETVDILIR
                     RDGQEKTIHWVFPGFTSAEFLGRFFNIWWLAFIFWLFGFVGQILIRPKDTRRLLLIAA
                     NYITSIWLITGNLSSSHLMESSTLMHATSWLLLPVYTHLHWIFPRPLNGLSKNVLIPV
                     YGVSGALALGEILHILPRSLYVIGFLGALIGSLVLELLHFIRQKDQRQNVTMLAAGVF
                     LAFAPTILFGLLILFSGFPALGPITFLALPFMPLTYFIVISRNQLGGVEVRANRLISI
                     YAFLIILGSVLLFLIMPITLLPLFEGETAFVTLAISSLTSLISILVFPAFQAYVEQRF
                     LGIKLPYQNIQESFSNRIAASASINDLLLLLENDVFPSLFIRQFAFIQTSNGNLNVLL
                     AKNVSADEMPDEGRISQLVERSGKFIPTGGWIRLILPLKAGDSFIGFWLLGRRDPDDH
                     YPQAEIVILQSIANQTAIALSYVLQTEYLKSIYQDGINQYEDERMRLALLLHDSILNE
                     LAVLRMNLNEMEEPPAFQNAYQKLTSQIREIIFNLRPPMLEYGLKPAIEEMAETLMEH
                     SQRKIKVITTIETDGSRYQAGNMEEHIFRMVQEASENSARHAQGQTIQIKGVLMKGEI
                     SLSIEDDGMGFNTKDGLGIDTLVRKKHFGLAGILKRAQLIGAEVFINSIPEKGTHVQV
                     VWKPSPIHG"
     assembly_gap    10370..10469
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10476..11159)
                     /locus_tag="PL000_17530"
     CDS             complement(10476..11159)
                     /locus_tag="PL000_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90735.1"
                     /translation="MTKKIRVAILDDHQSIVDGYIYRLEKDPQIEITATMAFGEELES
                     TLEKTPADVLLLDVNVPISKENQNPYPILHCIPKLLQTHPDLNILIISMHAERGLIRA
                     VMDAGASGYILKDDPAILKNLGNVVKIIAEGGVQYSQAAHDQYARYLSEEVLSPRQLE
                     TLSLSAAYPNSTTSDLANKLNVSHSTVRNLLSNAYFKLNVHNRAAAIAKARQMGLITP
                     NPPEATANQ"
     gene            11522..>11772
                     /locus_tag="PL000_17535"
     CDS             11522..>11772
                     /locus_tag="PL000_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90736.1"
                     /translation="MAGTQIREYLNPSNYDHFYELFHALSEVVLSKISVIRTACHILN
                     TLHAQTPQVFSNVDAATHLTAFNKFTVEFDADMRRFFWP"
BASE COUNT         2675 a         2823 c         3028 g         2946 t
ORIGIN      
        1 cactgactct tggtcaattc cctgccaatg ccgtatgtgg aaagttcatg atgtttgtat
       61 ctttcgatct tttcgttctt tgaaccgagc aggatatcca ccacatgtgc cgcgccgaat
      121 cgttccccgc tgcgcttcac gcaggacagg aatttttgca cagccaccgt gatgtcctgc
      181 tggtcgacgg gctcgttgtt gcagttgtca caggtgccgc aattttcctg cgcaaaggtc
      241 tcgccaaagt aggaaagcag cggcttgcgc ctgcaaatgc ttccttcggc ataacgggtc
      301 atcgcctcca agtgttgata ggaagcgctg cgttcaggtt cctctttctg gtcgatgaag
      361 taacggatct tgctggcatc gccatagttg tacagcagca gacaatgtgc gggcaggcca
      421 tcgcgccctg cgcgcccgat ctcttgatag tagctttcaa tgctcttggg tagatcaaag
      481 tggatgacaa agcgcacatt gggtttgttg atgcccattc caaaggcgat ggttgccacg
      541 atgatctgca catcatcacg gatgaatgcc tcttgattgc gcctgcgttc gtcatcttcc
      601 aaccctgcat ggtatgagcg gacggagtat tggtgccgcg ccagggtggc ggtgagatca
      661 tccacctgct tgcgcgaaaa acagtagatg atgcccgact ggtccttgaa gttctcaaga
      721 aaacgcaggg tctgtgcaat gggttcgcgt ttgggagata cttcgatgaa caggttttcc
      781 ctattaaaag aagcgaggaa ttcattggat tgtgtgaacc cgagggaggc ggcaatatcg
      841 gcacggactc gcggtgtagc ggtggccgtc agggccatgc atacggcaga gggaaatttt
      901 cggcgcacgt ccaccagttg gcggtattcg gggcggaagt cgtgtcccca ctctgagatg
      961 cagtgagctt cgtcgatcgc aagcagatca agtttcagcg aggaaagaag cgagtagatc
     1021 ctgggagtca gcaatgtttc aggcgccaca tacagcagct tggattgtcc gcttttcaca
     1081 gagtccatgt tttcctgata ttcctcttgt gagagcgaac tgttgaggaa aacggcaggc
     1141 acacccaggg cattgagttg ttccacctgg tctttcatca gcgcgatcaa aggggagaca
     1201 acaacggtca atccgtcaaa catcaacgcg gggacttgat atgtgagcga ttttccgccg
     1261 cccgtgggca tgacaaccag tgtgtcgcgc cgggcctgca cattggcaat gatctcacgt
     1321 tgcagcggct tgaaggtgtc gtaaccaaat atggatttta gaatggattc gggtgtgggc
     1381 atgtcggcct gattataatg ttactgcatt cgttttcagg ctttgttcca tgtaaaatga
     1441 gggatggaaa gaacatccaa atgtaaaaca aaagcattct tcgnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnccgcatg gtttggcggt gaattaagaa ccatcctttt
     1621 cgaacctctc tttttatatt gggtacactg atggcatcca ttccgatcag caagacaaag
     1681 atcatcccac cccggcgccg tgacgagttg ctcacccgca agcggctggt ggattttcta
     1741 ttcgattcgt tggataaaaa attgatcctt gtgtctgcgc ctgctggcta tggaaagaca
     1801 tctctgttga tcgattttgc aaaccagagt gacattgcct gttgctggct tgcgctcgat
     1861 gaaatggacc gtgacccaca gcgcttcctt gcttatttga tcgcagccat tgcagagcgt
     1921 tttccagatt ttggcgcggc atcggtttca atgcttggcg gcctgacctc ttttgagcag
     1981 gacatggaaa aaatgctggt gatgctggtt aatgaagcct atgagcagat ccacgaacat
     2041 tttattctgg tgttggatga ttttcacctg atcgaaaata ttcagccgat ccagtctttt
     2101 ctcaatcgtt tcctgcaatt ggtggatgag aattgccatg tggtcatttc ttcgcgggtc
     2161 ttgaacagcc tgcccgacct gcccttgatg gtggcgcgtg accaggtgag cggtttgggc
     2221 tttgccgatc tcgctttcca ccctgatgag atccaatcct tgctggcgca gaacaacaac
     2281 ctgcacatct cggatgacga agcgcgtaag atgatcgaaa ccacggaagg ctggatcgca
     2341 ggcctgcaat tttctggcat ggggtttctg ccgccttcgc acactggtgt gggtctttca
     2401 gatttcctcg gtcagcaagt gctggaccgt caacctgagt atctgcgtga tttcattttg
     2461 cgcacatcca tgctgggtga gttcgattca tccctgtgtg aaacggtcct tgctccgtta
     2521 tatgcagaac ctcaaaattg gcatcgcctg atcgccagtg tggtgcaaaa caatctcttt
     2581 gtgctgcccg tgggtgacga tggacgctgg ctgcgatatc accacctgtt ccgtgatttt
     2641 ttgcggaaac gcttcgagca gactcatcca gaagaggtcc gcccgatctt gcagcggctt
     2701 ggtcaggcct acgaaaacct ggccgaatgg gaaaaggcct actataccta caaacaatta
     2761 aatgatactg gtttgcaggc ggcattgatc gaacacgccg ctgccaccat gctgttgaat
     2821 gcccgcgtaa ctttggagtc atggctgaac gacctgcccc catccatgct gcggacacgc
     2881 cccggcctgc tttccatccg cggcacaatt ttggatacaa aaggacaggc acgcgaagga
     2941 ctcgacctgt taaatcaagc ggaacaggct tttcgtgcag aacaaaacgt gccagggttg
     3001 gcgcgggctt tgttgcgacg gggcagcgta caccgcttcc tcggagattt cgatgccacc
     3061 ctgcgtgatg ccgaagaatc catgcagttg accgaagcgg tggatgacct gcaggtcatg
     3121 tatgcggaag gtctgcgcct caaggcgttg gctttgtatc gtttgggtca tgcgcgtcaa
     3181 tcggtcagtt tctttgaaag ttcactgaat ctctacattc gtttaaagca tacccctacc
     3241 atcccgatcc ttttcatgga gacgggcatg gcctatcaaa ccattggaag ctatgccgag
     3301 gctggcactg cctacgataa ggcattgcag atctggcagc gtgatgggaa cctgctctgg
     3361 caggccagtt tgttgaataa ccttgccatc ctgcaccaat cctacgggga atatgagaaa
     3421 gccgccctcg cttttgaaaa tggattgctg tgtgcccgtc gcagcagcta tacccgcatg
     3481 gaagccctga tctccatcgg ccttggtgat atgtattccg aactggaaga tttcagcatc
     3541 gccctgacca attacgaaaa tgcagaagag atcgcccgca gaattaatga ccgcttcctg
     3601 attttttaca cgatcacggc acgggtcagg ctggccttgt tgcaaaaaga tgcggacggg
     3661 gcacatcgga tcatggaaca ggacgtggaa ctgtttcagt caaacgcatc atcccgctac
     3721 gaaagagggc tgctcgcgct catgcgcgga catctcgcac ttttgaccca tgaaaatgcg
     3781 gaagctgtgg ctcatctcga agaggctttg cgttgtttct ctgaagatgg ccgcaaactg
     3841 gaaggggata ccagccgtat ctggctggcg gcggcatctc atcaaaaccg taataatgag
     3901 ctggctgcac aggtgctcaa accacttttg ggaacacagc ccgctcaccc cgttcttgta
     3961 gcagttcatc aggcgcagat gtggctgaat ggactgcaaa ctcacccgga agttggccgt
     4021 atggtgggtg acctgttcat ccgtgccgct cgattctctg ccagaatccc cgccgacagg
     4081 cggcagcttc gccgcatggc acacgtgttg cagattccaa atccgcatat ggtcattcag
     4141 gcgtttggcc gggctgctgt cagtgtgggg ggcaagaccc tctctctttc cgattggcag
     4201 acccagtcag tgcgcgatct ctttttctat ttcctcaccg cctcgcgtgc cttcacaaaa
     4261 gaacagatcg ccgaaacgct ctggcccgaa gtggatgaac ctcacaagat caggctgcgt
     4321 ttcaaaaacg agatctaccg cctgcggcgc gcagttgggc aggagatcat tgcctacgaa
     4381 gatatcttct ataaattcaa ccgttcgctt gattttgaat atgatgtgga agctttcgaa
     4441 gcattccttg ctcatgccaa agccgccgcc acccccgttg aacagatcgg gtattacaaa
     4501 aaagcagtgg accttgtaca gggtccgttc cttgaggatg tgtatgccga ttgggccacc
     4561 ccggaacgtg agcggttaag tcaggtttat ctctcaaccc ttcttacctt gggcgacctt
     4621 cttttaaaac aaactcaaac cgagcaggct ttggcaatat gcaaacgggc attggattac
     4681 gatgtcacct ttgaggcagc atacgccctt tccatgcagg tgtatcaccg catgggcgac
     4741 cgtgccgcga tcgtgcggac cttccaaacc tgcgaggagg tgatgcagcg tgaacttggc
     4801 ctgccgcctt caagggaaac aaaagaactc tacaaacgcc tggtcatgta atctcatttc
     4861 aagataaaaa ttccgtacat ttgcaccgcg aagcccgcaa agggcgcnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnatt taatagaaga acagggcaca aagctcgtgg
     5041 agtactgaaa atccgcgagt tttgcgtctt gttttgtttt aacttaccca ctgcctttat
     5101 ctaattgtta cggcttttgg ctgctcaagc cgccgtcctc ggcagcgggg acggcggctt
     5161 ggaaatgcaa aaggaggttc attttcaggg gaagaacggc tcaaagtcgc agaatcagtt
     5221 ttcggcgggg atcgaatcgg aaattcctct taaaagcatg ggggtttgaa gcctgttttg
     5281 aagcctggtg gctcgtatcc tgttcgcaac gaaaggatgt gtaccatgaa acaacaggct
     5341 gcctccatcg gtatttttct tcagagaata gatcgccagc aaattcagtt aattcttgcc
     5401 attgcttctc ttgtggcgct ggcccttggg tctggtgctc ctcttgatgg cggcagcggc
     5461 ggcggataag tgatcttaat tttattcgtt gctgttgggc tcctggttgg ccttttatgg
     5521 gcgtggttcc tgaaaacgcc ctaccatgga cctgatctga aggccatgtg gctggctctt
     5581 ttggggttcc tgcctcaaat tttggttgcc cgcctgccca accttttgcc aaaccgcatg
     5641 gatgcggcca gcctcaccat ctcacaggtc gcgctgcttg ggtttgcatg gctaaatcgg
     5701 catgttgctg gaatgtatct tttcatctgt ggcgcagcgc taaatctgct cgtcatgggg
     5761 gcgaacggcg ggttcatgcc cgtcagcccg caaactgccg accgcctgtt ttcagaagaa
     5821 gccctgttgg aactttcacc cggggaccgc atcggcccga aggatattct cctgcttccg
     5881 cagcaaaccc gcctcgaatg gcttgcggat cgtttcctac ccccggcctg gttcccgttt
     5941 caagcggcat tcagcttcgg tgacgttttt atcgcggtcg gcgtcttttg gatgttggtc
     6001 cgcccaaata ctttatcagg aaatagaggt acatgacatg cttgccccca tcaccatccg
     6061 aagaccagca tctgtaaaca tcactgcggc gcgtccgggc gtttcgcggg gattaagccg
     6121 cgttttttca gcggcggttg ttaacaagca gttttgcgag ttgctgctca aagaccccca
     6181 gaaggcttta agtagagggt atctgggtga atcgtttttg ctcagcaagg aagagtttga
     6241 cctgattctc tccatccgcg ccagttcact ttctgatctg gccaggcagg ttaatcgtta
     6301 tcaaaatggc aattgacatg catgatcgat tccgtccccg cagacaatgt atctgatgct
     6361 ctcttttccc aattttaaat atagaggtct gcctggagtc tttctttaat ggcgctttta
     6421 aaaaaaatga ttaccgaact ctctttgcaa aacctgccag atgccagccg ccaactttgg
     6481 gctgtctttc aggcctttga cgaccagcta tttgtcctcg atgcagatgg cgtgattttg
     6541 gatttcaagt tgggtggtca ggtcagccac ctgccggtgt caatggacat gctgttgatg
     6601 cggaaaattc agaacatctt ccccgctgag ataggcgcga aacttgccga cgtgttgaac
     6661 aaaatccgca ctgggagcag ggtctctcac ctcgaatact ccctgcaaac gccttctggc
     6721 aggctgtggt tcgaatcacg actggtggct cttgccaatg gacagatcat catgatggtg
     6781 cgcgatatca cccgccacaa gatatctgaa gaaacgatca agcgccagtt tgggcagttg
     6841 gccgccctgc gctccattga tatggcgatc acctctgacc ttggcttgaa accagttctt
     6901 tcgatgatcc ttgaacaaac ccgtatgcaa ttgggtgtgg atgccgccag cattctgttg
     6961 ttcgacccgc agacaaatct gcttgaattt gccgcagatg cggggttctt cacaaaagct
     7021 ttgcagcata cccgtctgct ggttggggag gaacttgccg ggcgggccat acaggatcag
     7081 tcggtggtct atgttgataa cctgctcgaa cagcccacgg catttcaaag atcgccccag
     7141 tttcggctgg aaaatttcat ctcctattat gctgtgccgt tgatttccag agggcaaccc
     7201 gttggcgtgt tggagatttt ccatcgtgcc cgccttgaaa cagatcagga atggctgaac
     7261 tatatggaga cccttgcagg gcaggcagcc attgccattg ataatggcac gcttttcaag
     7321 caccttcaac aatccaatgc ggaattgatg ctggcctacg acaaggtcat cgaaggctgg
     7381 tcgcgggcat tggatctgcg caacaaggaa accgaagaac attcgcgccg tgtgactgaa
     7441 ttgaccgtca ggctggcaga acgcatggga attgttcagg gcgaacttac acacatccat
     7501 cgcggcgcca tgctgcatga tatcggcaag gttgcaattc cagatagcat cctgcttaaa
     7561 cctggtccct tgaccgatga agaatggacg gtcatgcgga gccacccgct gatcgcggtg
     7621 gaactgctgg caccgatcga gcatctcgtg cccgctttgg atattccccg ctcacaccat
     7681 gaaaagtgga atggtgaagg ctacccggac caactttcag gctggggaat tccactctca
     7741 gcccgcattt tttcagtggt ggatgtgttc gatgccctca tatcagatcg tccctaccgc
     7801 ccgggttggc cggtgggtaa ggctctgcaa tatatcaagg aacaatcaga aatacatttt
     7861 gacccgcatg ttgtgcccat tttcatcgat atgatggaaa gccgggttta atttcaacat
     7921 accgatttga acaaaggggg aaaggggata gggtgcgctt caacttcttg ggtggctggt
     7981 catcattgga gcaagagtgg tgaggttgaa gcgcgcctgg atatttacga gattacccat
     8041 gtatggggga aggcttccaa acaacctgaa catgggtgcc tttttcgggg attgaattga
     8101 tgaaaacctc cgccccgatc aactgggcgc gtttcaaaat tcccgccaga ccgaaatgct
     8161 ttttcctgac aagggtatca attcccaacc cgtcttttgt attaaacccc atcccgtcat
     8221 cttcgatgga gagtgagatt tcacccttca tcaacacacc cttgatctgg atcgtctggc
     8281 cctgtgcatg gcgtgccgaa ttttcactgg cctcctgcac catgcggaag atatgttcct
     8341 ccatgttccc agcctggtat cttgagccgt cggtttcaat cgttgtgatg accttgatct
     8401 tccgctggct gtgttccatc aaggtttctg ccatttcttc aatggcgggt ttcaacccgt
     8461 attccagcat gggcgggcgc aggttgaaaa tgatctcacg gatctgactc gtcagctttt
     8521 gataagcatt ttggaaagca gggggttctt ccatttcatt caggttcatg cgcagtacag
     8581 ccagctcatt gaggatgctg tcatgaagca gcagcgccag tcgcatgcgc tcatcttcgt
     8641 actgattgat cccatcctga tagatggatt tgagatactc cgtttgtaac acgtagctta
     8701 atgcaatggc ggtttggttt gcgatggatt gcaggatgac gatctctgcc tgtggatagt
     8761 ggtcgtccgg gtcgcggcgt ccgagcagcc aaaagccgat gaaagagtca ccagctttca
     8821 gggggagaat gaggcggatc catcctcccg tggggatgaa tttcccgctt cgttctacca
     8881 gttggctgat tctgccttcg tctggcattt catccgctga gacatttttt gccagcagca
     8941 cattcaaatt cccattggag gtttgaatga aggcgaattg acggatgaac aaacttggaa
     9001 atacatcgtt ctcaagcagc aatagaagat cgttgatgga tgcactggcc gcaattcgat
     9061 ttgaaaaact ttcctgtatg ttttggtagg gaagtttgat tccgagaaat cgttgttcca
     9121 cataggcttg aaatgctggg aaaaccagaa tcgagatcag gctggtgaga gaggaaatgg
     9181 ccagggttac aaatgccgtc tcgccttcaa acaatggtaa aagcgtgatg ggcataatga
     9241 ggaacagtaa aacggagccg agaatgataa gaaaggcata aattgaaatc aggcggttgg
     9301 cgcgaacttc caccccgccg agttggttgc gtgaaatgac gatgaaatag gtcaggggca
     9361 tgaatggcag ggccaggaat gtgatggggc ccagggctgg aaacccgctg aataaaatta
     9421 acaacccaaa caggatggtt ggtgcaaaag ccagaaagac accagccgca agcatggtca
     9481 cattttggcg ctgatctttt tgacggatga aatgaagcaa ttcaagcacg aggcttccga
     9541 tcaatgcccc aagaaaacca atgacatata aactgcgcgg caggatgtgc aatatctcgc
     9601 ccagtgccaa agcaccgctc acaccgtaaa cagggatcaa cacatttttt gataacccat
     9661 tcaaaggacg cgggaagatc caatgcaggt gggtgtagac aggcaggaga agccagcttg
     9721 tggcgtgcat cagggtggaa ctttccatga gatgcgagga ggataaatta ccagtgatca
     9781 accagatgct ggtgatgtag ttggccgcaa taagcaggag cctgcgcgtg tcttttgggc
     9841 ggatcaagat ctgtccaacg aatccgaaca gccaaaaaat aaaagccagc caccaaatgt
     9901 tgaaaaaccg cccgaggaac tcggcgctgg taaagcccgg aaagacccaa tggattgttt
     9961 tttcctgccc gtctcgacga atcaaaatat caaccgtttc ttcggggcgt gtgttttcga
    10021 agaatgacag cctgcggttc tttcgaaatt catcgagtgt aatatccccg acctttatga
    10081 ggatatcgcc gatttgcagg gaggtttcag gcgtggtctt tacatagacc atgtccacgc
    10141 gcccttctga agggttgaaa taaaaccctg cataaggcat cctgaataaa aaggcatagg
    10201 aaaaaaatat caggaagatc aatatcagcg cggaaaggga tgtttcaatc ttttccccaa
    10261 tctggagatt gtttttgttc atgggtttcc tggctggaaa tgggtgggtt gataagggat
    10321 aggaggccgt ttcgattata gcgtaggaac atgccatgag caccactttn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctgtttatt gattggctgt tgcttccggc
    10501 ggatttggtg taattagccc catttgccgc gcttttgcga tggcggcggc gcggttgtgt
    10561 acattcaatt tgaagtaggc gttggagagc aggttgcgga ccgtggaatg gctcacgttc
    10621 aatttgttag caaggtcaga tgttgtggaa ttggggtatg ccgcgctgag agagagtgtt
    10681 tccagttgac gagggctgag aacttcttcc gataaataac gggcatattg atcgtgtgca
    10741 gcctgactgt attgaacccc gccttctgca atgattttca ccacattgcc cagattctta
    10801 aggatggcgg ggtcgtcttt caaaatatac ccactggccc cggcatccat cactgcgcgg
    10861 atcaatccgc gctcggcgtg catgctgatg atgaggatgt taagatcagg atgcgtttgc
    10921 agtaatttgg gaatgcaatg caggatcggg taggggtttt gattctcttt tgatatcggg
    10981 acgttgacat ccagcagaag cacatctgca ggtgtttttt ccagcgtaga ttccagttcc
    11041 tccccaaacg ccatggtcgc tgttatttcg atctgcgggt ctttttccag ccgataaatg
    11101 tacccatcca cgatactttg atgatcgtcc aggatcgcca cgcgtatttt ttttgtcata
    11161 taccacactc ccgcttgcat ttaattcagg gtagcagaaa gaatcctcgg tttcaagcca
    11221 gtatgcagga ttcagtacaa atgtctatgc ctggttgcac ctgtctattg aatcgccttt
    11281 tgggtatctg tacaatttac ttaagaatat caggcttaat catggatgaa atgagccatt
    11341 gctctcattc cattcatcat ggggaaaagg ggataggatg cgcttcaact tcccgggtgg
    11401 cttgaacgtc agaagactgg cggaggaggt tgaagtgcgt ctggatatgt tcatttaaaa
    11461 gaaggtggga tagtgcgtgg attgagaaaa caaaacccgc agcgggtgat acaatccctg
    11521 catggcagga acacaaataa gggaatacct gaatccatca aactacgacc atttctacga
    11581 gctttttcat gcattatctg aagtggtgtt gagcaagatc agtgtgattc gcacagcctg
    11641 tcatattctg aacacccttc acgctcaaac cccgcaggtt ttttcaaatg ttgatgctgc
    11701 tactcatctg accgcattta acaagttcac tgtggaattt gatgcggaca tgcgcaggtt
    11761 tttctggccg ca
//
LOCUS       DAOQLC010000128        11732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2105_length_11732_cov_24.544489, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000128 DAOQLC010000000
VERSION     DAOQLC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2105_length_11732_cov_24.544489"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PL000_17540"
     CDS             complement(<1..1652)
                     /locus_tag="PL000_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNC90737.1"
                     /translation="MIHLTSLNPNVELHLSDGRILSGPRGTQVGEFLRAVKDDFSAPI
                     VAAILNNEIHELTYPIERESDCAPVTMDRADGARIYRRSLIFLLEIAFAELFPKGKLT
                     IDHSVSSGGFYCQVTEREALTQKELDALKTHMEKIVAEDQAFERKQIPIQEAIQYFEK
                     LGYSDKVRLFSHRQKDYLTLYSLNGRMDYLHGYMVPSTGYLRWFDLNCIDGGFTLQFP
                     RRHAPTHLEPMGDYPKLLNTFRQYGDWLTRLAIDNVGALNDAIVAGRADELIMVSEAL
                     HEQHVADIAQQIAQKNSRIILIAGPSSSGKTTTSRRLAIQLLARGISPYPLELDNYFI
                     DRSKTPLDEEGKPDFEALEALDLNRLAQDIEKLLRGEKVQLPRYNFKSGLSEMGDVIQ
                     LKDGQPLILEGIHGMNPRLIPDRWSGAAFRVYVSALTQLNLDRHNRVSTTDTRLIRRM
                     VRDARERGYSATQTISRWESVRRGEKRHIFPYQENADVMFNSALVYELAALKPLAEPL
                     LRQVQHRSPEYIEARRLLAFLEWFLPLDVNLIPANSIVREFLG"
     gene            complement(1674..2774)
                     /locus_tag="PL000_17545"
     CDS             complement(1674..2774)
                     /locus_tag="PL000_17545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNC90738.1"
                     /translation="MQSSISNVKATDQLKVLVFGAGAIGTYYGGSLALAGHHVVFVEQ
                     PKMVEQLRERGLRLDLTIDERRKTKDAFVVEPRSFVIEPSLEEALKFGPFDVALFALK
                     SFDTVSALEGMKPFAEKLPPILCLSNGVENEQAIARTLGADKVIYGTVTTAIGRRGAG
                     DIVLEKLRGTGIAAGHVLSEKLVAAFQNAYLNCRLYPEPHGMKWSKMLTNLIANPTSA
                     ILNMTAAQVFANRKLYKLEIAMLKECLDVMKAQNLEVVDLPGTPVKLLALAAYLPLWL
                     SKPFLSRAAGSGRGGKMPSFHIDLYAGRGQSEIEYLHGAVVREGKKHGVPTPVNEVLT
                     TTLLALTNKEIPLEEFAGQPEKLLANVLTLKR"
     gene            complement(2835..3479)
                     /locus_tag="PL000_17550"
     CDS             complement(2835..3479)
                     /locus_tag="PL000_17550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC90739.1"
                     /translation="MSESRPQPFQITLLRHGESTGNAESRWQGQADFPLNDTGRAQAR
                     ALAERWKSENMKFDFVISSPLGRAKETAEIVASCLDLKVEFDPLWLERDNGEFAGLTA
                     HEVRQNFQHPAFSTPYDPVGVDGEGDWELFLRAGQALHSLLKREPARYLIVSHGGLLN
                     QIMHAVVGLAPQANNAGTRFRFENTAFAQLTYYPHQHRWAIDKLNDHAHWRDAE"
     gene            complement(3476..4417)
                     /locus_tag="PL000_17555"
     CDS             complement(3476..4417)
                     /locus_tag="PL000_17555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC90740.1"
                     /translation="MSSNTTVDLVIPVFNEEGVIEHTHKRICAVVDSLAYDFKIIYVD
                     DGSRDGTVASLRKIADSDQRVILLQLSRNFGHQAALTAGMDASQSEIAITLDGDGQHP
                     PEMIPQMLNLISQGYDIVQTQRMDEAQPASFKKWTSGLFYRLINVISGTQVLPGAADF
                     RALTRPALNALKAMPEYHRFLRGMVSWIGFSMVILPYQPDERIGGVSKYSLSKMIRLA
                     MDAVFSFSLVPLYVGLSLGGILLCLAMVEMVYVLSFWVTGQTSNLAPGWSSLMFVILI
                     VGGMLMALLGFIGVYVGYIFQEVKRRPVYLVKGEMKR"
     gene            complement(4445..6151)
                     /locus_tag="PL000_17560"
     CDS             complement(4445..6151)
                     /locus_tag="PL000_17560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNC90741.1"
                     /translation="MNRNEILEQLKANPKISVLIVGAGINGIGTFRELALNGVDVLLV
                     DRGDFCSGASAASSHMAHGGIRYLENGEFRLVREAVQERNRMIQNAPHIVQPLPTTIP
                     IFKYFSGLFNAPLKFLGWLDKPSERGSVIIKLGLIMYDAYTGKTRTVPRHQFFSRAEA
                     LKNWPRLNSEIVNTAKYYDGLISNPERLALEVMLDGEAENPNARAVNYVSMVKAEKDT
                     VILRDELTDQTYEVRPELVINAAGPWIDFVNHSMGLSTRSIGGTKGSHIVIDHPELRA
                     AIGENEFFFENKDGRIVLIFPLQDRVMIGTSDIKIDNPDEAQCTDEEVDYFIGLVARV
                     FPDIKVSREQIVFRFTGVRPLASSGAKTTGQYSRDHHIEVLNGDWTNLGFPIYSLVGG
                     KWTSFRAFSEQVADKALAFLGMPRQKDTRDLAIGGGRGFAVDPDGQKRQIEGFAAWTG
                     LDKERLQTLYLRYGSRTEAVANFMKRAADEPLKSLPSYTRREIMFLAQYEKVARLDDL
                     LLRRTMLAMLGRLTKESVLETADVLGEALGWGSDQKTAEVQRTLRLLRDRHGVVLNEA
                     KS"
     gene            complement(6258..7751)
                     /gene="glpK"
                     /locus_tag="PL000_17565"
     CDS             complement(6258..7751)
                     /gene="glpK"
                     /locus_tag="PL000_17565"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNC90742.1"
                     /translation="MSKYVAAIDQGTTSTRFIIFDHGGNVIAVDQKEHRQIYPKPGWV
                     EHDPLEVWERTQEVMKGALQKSGIQAADIAAIGITNQRETAVVWDKSTGKPVYNAIVW
                     QDTRTDVICNELAKVGGQDRLRSKTGLPLATYFSGPKIKWILDHVEGARAKAEKGELL
                     FGNMDTWVIWNLTGVHVTDVTNASRTLLMNLNTLDWDDELLNLLDVPRAMLPEIKSSS
                     EVYGHVTQSGLSALQGIPVAGDLGDQQAALFGQTCFSAGEAKNTYGTGCFMLMNMGEI
                     PVPSKSGLLTTLGYKIGNQKAVYALEGSIAITGALIQWLRDNLGLIQSSAEVESLASS
                     VEDNGGIYFVPAFSGLFAPYWKSDARGAIVGMTRYVTKGHIARAALEATAYQTCEVLD
                     AMEADSGVKLTALKVDGGMVFNELLMQFQSDILNVPVVRPKVAETTALGAAYAAGLAV
                     GFWKDYDELRANWGRDKEWTPQMDTTLRGKLYAGWKKAVTRTFDWVE"
     gene            complement(7948..9570)
                     /locus_tag="PL000_17570"
     CDS             complement(7948..9570)
                     /locus_tag="PL000_17570"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNC90743.1"
                     /translation="MQDDLLAAKPPQNTSMDDPIAARRTFAIISHPDAGKTTLTEKLL
                     LYGNAIELAGNVRARKNQRSTTSDWMEMERERGISITSTILQFPYQSNIVNLLDTPGH
                     QDFSEDTYRTLSAVDSAVMVIDAAKGIEPQTLKLFEVCRKRGIPIFTFINKMDRPARD
                     PLELLDEIKKVLGMEPVPMNWPIGDGPEFRGVYDRASKGVYLFERTERNERMAPEIFV
                     PLDELGKNNILPQKRYDELVESVALLDEVGVVFDHDLVLQEKQTPVFFGSAVTNFGVR
                     LFLDTFVKYAPQPQPYLSDRGAVVPQDPGFSGFIFKIQANMNPKHRDSVAFLRICSGR
                     FERGMDLVHAQSGKTVRLVRPYKLFASEREIIDEAYPGDVLGIPNNGEFAIGDSLCTG
                     ELVRFAPIPRFQPEHFALLRNTDLGKQKQFQKGLLQLESEGAVQVLFNVNAFKREPIL
                     AVVGQLQFDVVQARLKAEYNVPTELERLPHVMMRWIKGPETAIQNITQRSEVIIARDS
                     REEWAAIFSTPFFLKYFIEKNPDLRFVEITEN"
     assembly_gap    9637..9736
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9777..10541)
                     /locus_tag="PL000_17575"
     CDS             complement(9777..10541)
                     /locus_tag="PL000_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HNC90744.1"
                     /translation="MFNSKVFLAEFIGTFALVFIGMGAGIVNAGLLGVALAHGFTLAV
                     FAYAYGHISGTHVNPAVTFALALKNVVTWGQAVVYWIAQFAGAILAAFVMKLFLDSLG
                     GPIDAGATTGALTDKAPILAMVMEAILTFFLVNTILHTAVGGKGGRFAGWAIGTTLAL
                     AILGGGPFTGASLNPARTLGPAVFASIPSLTSPNTYLIYLFGPLIGATLAVLVYNFFT
                     SSDEIEEDEEEVVEEVPAVVEEEAPVKKSRSRKSEE"
     gene            complement(10596..11525)
                     /gene="fmt"
                     /locus_tag="PL000_17580"
     CDS             complement(10596..11525)
                     /gene="fmt"
                     /locus_tag="PL000_17580"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC90745.1"
                     /translation="MTLRIVFMGSPDFALPSLSTLAAEPNYQVVGVVTQPDRASGRGR
                     ELKPPPVKSLALELNLPVMQPEKLRQPEAMEQLRAWEPDLIVVAAFGQILKKDVLDLP
                     RHGCINVHASLLPRWRGAAPINAAILHGDEETGVTIMQMDVGLDTGPMLAKRSVRIKP
                     DDTAGSVSQALSTLGADLLIETLPAYLAGNLTATPQPEDGFTYAPMLKKTDGLLDFTQ
                     PAIDLERRVRAMNPWPGAWFEWNGNPLKVLRASTVSGEKGLASGSRFTVEGRPAVQTS
                     AGALVLDEIQPAGKKAMPGKSFLAGARDWVAVA"
BASE COUNT         2817 a         3171 c         2985 g         2659 t
ORIGIN      
        1 cccaaaaatt cgcggacaat ggaattggcg gggatcaaat tcacatccaa tggcaggaac
       61 cattcgagga aggcgagcag ccgtctcgct tcgatgtatt cgggcgagcg gtgttgcacc
      121 tgccgcagga gcggctcggc cagcggtttg agcgcggcga gttcatagac cagcgcagag
      181 ttgaacatca catccgcgtt ttcctgatag gggaagatgt gccgcttttc accgcggcgc
      241 accgattccc aacggctgat ggtctgcgtg gcggaatatc cgcgttcgcg cgcatcgcgc
      301 accatgcggc ggatgaggcg cgtatccgtt gtggaaactc tattgtggcg gtccaggttg
      361 agctgtgtca gggctgagac atatactcga aacgccgcac cagaccaacg gtcagggatg
      421 aggcgggggt tcatcccatg aatgccttcg aggatgagcg gctggccgtc tttcaattgg
      481 atcacatccc ccatctcgct caaccccgac ttgaagttgt atctcggcaa ttgaactttt
      541 tctccccgca gcagtttctc gatatcctgc gcgaggcggt ttagatcaag cgcttcaagc
      601 gcttcgaaat cgggtttgcc ttcttcatcg agcggagttt tcgagcggtc aataaaatag
      661 ttatccaatt caagcggata cggtgaaatg ccgcgcgcaa gcaattggat cgcaaggcgg
      721 cgcgaagtgg tcgtttttcc tgaagaggat gggcccgcga tcaaaataat gcgggaattt
      781 ttttgcgcga tctgctgggc gatatcagcg acatgctgct cgtgaagcgc ttctgaaacc
      841 atgatgagtt catcggcgcg tcctgccaca atggcatcgt tgagtgcgcc gacattgtca
      901 atggcgaggc gcgtcagcca atcaccgtat tgacgaaagg tgttaagcag ttttggatag
      961 tcgcccatcg gctcaaggtg tgtgggcgca tggcggcgcg gaaattgaag tgtaaaacca
     1021 ccatcgatgc aattgagatc gaaccagcgc agataccccg tggagggaac catgtaaccg
     1081 tgcaggtaat ccatacggcc gttgaggctg taaagggtga ggtaatcttt ttggcggtgc
     1141 gaaaacaagc ggactttgtc tgagtagccg agcttttcaa aatattgaat ggcttcctga
     1201 atcggaattt gtttgcgttc aaaggcctgg tcttcagcca cgatcttttc catgtgagtt
     1261 ttgagcgcat caagttcttt ttgcgtcaac gcctcacgct cggtgacctg acaataaaac
     1321 ccgcccgatg aaacggaatg gtcaatggta agtttgcctt ttgggaaaag ttcagcgaat
     1381 gcaatttcaa gcaggaaaat cagcgaacga cgatagatgc gcgcgccatc ggcgcggtcc
     1441 atggtgacgg gggcgcaatc tgattcacgt tcgatgggat aggtcaattc gtgaatttcg
     1501 ttgttgagga tcgcggcaac aatgggagcg ctaaaatcat ctttgactgc acgaaggaac
     1561 tcccccacct gtgtgccgcg tggacctgac aagatgcgtc catcggagag atggagttca
     1621 acattgggat tgagagaagt gaggtgaatc atgggtttta acgtcgaaac gttttaacgt
     1681 tttaacgtta aaacgttggc caatagtttc tctggctgac ctgcaaactc ttccagcgga
     1741 atttccttgt tggtcaatgc cagcagggtt gtagtcaaga cttcattgac gggggtgggc
     1801 acgccatgtt tctttccttc gcggacaact gcaccatgca gatattcgat ctcgctttgg
     1861 ccgcggcctg cgtacaggtc aatgtgaaag gagggcattt tgccgccgcg tccactgcct
     1921 gcggcgcggg aaaggaacgg tttggacagc cagagcggca gatacgcagc caacgccagc
     1981 aacttgacag gtgtaccggg cagatccacg acctcgagat tttgcgcctt catcacgtca
     2041 aggcattcct tgagcatggc gatctcgagt ttgtataatt ttcgattggc aaagacttgt
     2101 gccgctgtca tattcaagat cgcagaagtt ggattcgcaa tgagattggt caacattttt
     2161 gaccatttca ttccatgtgg ttcgggatac aaacggcaat tgagataggc attctgaaat
     2221 gcagcaacga gcttttccga aaggacatgc cccgccgcaa tgcctgtgcc gcgtagtttt
     2281 tcgaggacaa tatcccctgc tccacgcctg ccaatggcgg ttgtgacggt accgtaaatg
     2341 accttgtctg cgccaagcgt gcgcgcaatc gcctgttcat tttccacgcc atttgaaagg
     2401 caaagaatgg gcggaagttt ttcggcaaag ggcttcatcc cttccaaggc agacaccgta
     2461 tcgaaggact tcaacgcaaa aagcgccacg tcaaagggac cgaatttcag cgcctcttcc
     2521 aaagagggtt cgatcacaaa ggaacgcggc tcaacaacaa aggcgtcttt tgtcttccgt
     2581 ctttcatcga tcgtcaggtc aagcctcagt ccgcgctcgc gcaactgctc caccattttg
     2641 ggctgttcca cgaaaacgac atgatgaccg gccagcgcaa gcgacccgcc ataatatgtc
     2701 ccgatcgcgc ctgcgccgaa gacaaggact ttcaattgat ctgttgcttt tacattggaa
     2761 atggatgatt gcaaaggaac ctcggggaaa gaaaaggatg aaagatgaag gatgaataaa
     2821 acctgtttac ctgtctactc cgcatccctc caatgcgcat ggtcattcaa tttatcgatc
     2881 gcccagcgat gctggtgcgg ataataggtc aactgggcaa aagcagtgtt ctcaaagcgg
     2941 aaacgggtgc ccgcattatt ggcctgcgga gccagcccga ccacggcatg catgatttga
     3001 ttcaacaaac cgccatgcga aacgatcaaa tatcgcgcgg gctcacgctt gagcaggcta
     3061 tgcaatgcct gacccgcacg caggaacaac tcccaatccc cttccccatc cacaccaacg
     3121 ggatcgtagg gcgtggaaaa agcagggtgc tgaaaattct gccgaacttc atgcgcggtg
     3181 aggcctgcaa actcgccatt gtcacgctcc aaccagagcg ggtcaaactc cactttcaaa
     3241 tccaggcatg aagcaacgat ctctgctgtt tcttttgccc gtcccagcgg actggagatc
     3301 acaaaatcga acttcatgtt ttcagatttc cagcgttcag caagcgcacg cgcctgtgcg
     3361 cggccagtat cgttgagcgg aaaatcagcc tgaccctgcc aacgggattc ggcattaccg
     3421 gtcgattcac cgtgacggag cagggtaatc tgaaaaggct gtggacggga ttcgctcatc
     3481 gtttcatctc ccctttcacg agatacacag gtctgcgttt gacttcctga aaaatataac
     3541 cgacatatac gccaatgaaa cccaacaagg ccatcaacat gccgccgacg atcaggatca
     3601 cgaacatcaa cgaactccaa cccggtgcaa gattcgaagt ctgccctgtg acccaaaacg
     3661 agaggacgta caccatttcc accatggcca ggcagagcaa aattccgcca aggctcaaac
     3721 ccacgtacaa gggcacaagt gaaaatgaaa agaccgcatc catcgcaagg cggatcattt
     3781 tactgagcga gtatttcgaa acaccgccaa tacgttcatc gggttgatac ggaaggatga
     3841 ccatcgaaaa cccgatccac gaaaccatgc cgcgcaaaaa acgatgatac tcgggcatgg
     3901 ccttgagcgc attgagcgcg gggcgtgtga gtgcgcggaa gtctgcggcc ccgggcaaaa
     3961 cctgcgtccc gctgatgacg ttgatcaggc gatagaacag gcccgaggtc cattttttga
     4021 acgaggcggg ttgggcttcg tccatgcgct gggtttgcac aatgtcatac ccctgcgaaa
     4081 tcaaattcaa catttgcggg atcatctctg gcgggtgctg cccatcgcca tcgagcgtga
     4141 tcgcgatctc actttgcgag gcgtccatac cagccgtcag cgcggcttga tgcccaaagt
     4201 tgcggctgag ttggagcagg atcactctct ggtctgaatc tgctatcttt cggagcgaag
     4261 caactgtccc gtcgcgtgaa ccgtcatcca catagataat tttgaaatca taagcaagcg
     4321 aatccaccac ggcgcagatc cgcttgtgag tatgttcgat cacgccttct tcattgaaga
     4381 cggggatgac aagatcaact gttgtgttgg aagacattac aggattctac catgcgccct
     4441 tggctcagga cttggcctcg ttcaggacaa ccccgtgcct gtctcggagc aaacggagag
     4501 tccgctgcac ctcggcagtt ttctggtcac tgccccaacc gagtgcttcg cccagaacat
     4561 cggcggtttc aagcacggat tccttggtaa ggcggccgag catggccaac atggtacggc
     4621 gcaaaagcag gtcatcgaga cgggcgacct tttcatactg cgcaaggaac atgatctcac
     4681 gccgcgtgta cgaaggcaga gacttcaacg gctcatcggc ggcgcgtttc ataaaattgg
     4741 caaccgcttc agtgcgcgaa ccgtaacgca ggtacaaagt ttgcaggcgt tccttgtcga
     4801 ggccagtcca tgctgcgaag ccttcgatct gacgtttctg cccatcagga tcaactgcaa
     4861 atccgcggcc gccgccaatg gcaagatcgc gcgtatcttt ctgacgcggc attcccaaaa
     4921 acgccagtgc cttatcggcc acctgctccg aaaaagcgcg gaaggatgtc cacttgccgc
     4981 caacgagaga gtaaatgggg aaaccgagat tcgtccaatc gccattcaac acttcgatat
     5041 gatgatcgcg gctgtattga cctgtggtct tcgccccgct gcttgccagc ggacgtaccc
     5101 ccgtaaagcg gaagacgatc tgttcacggc tcactttgat atcagggaaa acgcgcgcca
     5161 ccagtccgat gaagtaatcc acttcttcat cggtgcattg cgcctcatcg ggattatcga
     5221 tcttgatatc tgatgtgccg atcatcacgc ggtcttgcaa ggggaagatc aagacgatgc
     5281 gaccatcttt gttctcaaag aaaaattcat tttcgccgat ggcagcgcgc aattcaggat
     5341 ggtcaatgac gatgtgcgag ccctttgtgc cgccaatgga acgggtggaa agacccatgc
     5401 tgtggttgac aaaatcgatc cacgggccag ccgcattgat gaccagttcc gggcgcacct
     5461 cgtaggtttg gtcggtcaat tcatcgcgca aaatgacggt atctttttca gccttcacca
     5521 tgctgacgta atttaccgcg cgcgcattgg ggttctcggc ttcgccatca agcatcactt
     5581 ccagcgccaa ccgttcgggg ttggagatca gaccgtcgta atatttggcg gtgttcacaa
     5641 tttcagaatt caggcggggc caattcttca gcgcttcggc acgcgaaaaa aattgatggc
     5701 gcggcacggt acgggtcttg cctgtgtagg catcgtacat gatcaaacca agtttgatga
     5761 tgaccgatcc gcgctcggag ggtttatcga gccagcccaa aaatttcagc ggcgcattga
     5821 acaaccctga aaaatattta aagatcggaa tcgtcgtcgg cagcggctgc acaatatgcg
     5881 gcgcattttg gatcatgcga ttgcgctcct gcaccgcctc gcgcaccaga cgaaactcgc
     5941 cgttctccaa ataacggatt cccccgtgcg ccatgtgcga agaagccgca ctcgcacccg
     6001 agcaaaaatc gccgcgatct acgagtaaaa catccacacc gttcaaagcc agttcacgga
     6061 atgtgccaat gccattgatc cctgcgccta cgatcaacac cgagatcttt gggtttgctt
     6121 tcagttgttc gagaatttca tttcggttca tttttattat ccagtttcat gttcaaaggt
     6181 tgaaggttac aggttgaagg tttcaggttt caaacttgca acattcaacc tgcaacttgc
     6241 aacccactca caacccgcta ctcaacccaa tcaaacgtgc gtgttacggc tttcttccag
     6301 cctgcatata attttccacg cagggttgtg tccatctgcg gagtccattc cttgtccctg
     6361 ccccagttgg cgcggagttc gtcgtaatcc ttccaaaaac ccaccgcgag cccagccgca
     6421 taagccgcgc cgagggcggt cgtctctgcc accttcgggc gcaccacagg cacattcaaa
     6481 atatccgact ggaactgcat cagcaattcg ttgaacacca tgcccccatc cactttcaac
     6541 gcggtcaact tcacacccga gtcggcttcc attgcgtcga gcacttcgca ggtctggtag
     6601 gcggtcgctt cgagggccgc ccgcgcgatg tgacccttgg tcacgtaacg cgtcatgccg
     6661 acaatggccc cacgcgcatc cgacttccag tatggagcga acaaccccga aaacgcaggg
     6721 acaaaataaa tgccgccatt atcttcgacg gatgaagcca acgattccac ctccgccgac
     6781 gactggatca gccccaagtt atctctcagc cactggatca gcgctcccgt gatggcgatc
     6841 gaaccttcca acgcgtaaac agctttctgg tttccgatct tgtacccgag cgtagtcaac
     6901 agccccgatt tcgaagggac gggaatctcg cccatgttca tcaacatgaa acagcctgtg
     6961 ccgtaggtgt tcttcgcctc ccctgcactg aagcaggtct gcccgaaaag cgcggcctgc
     7021 tgatcgccca agtcacctgc aacagggatg ccttgcaggg cactcaagcc gctttgagta
     7081 acgtggccat atacttccga agatgacttt atctcaggca gcatcgcgcg cggcacatcc
     7141 agcagattta aaagttcatc gtcccaatcg agggtgttca aattcatcaa caatgtgcgt
     7201 gaggcattgg tcacatcggt gacgtggacc ccggtcaaat tccagatgac ccaggtatcc
     7261 atattgccga agagcaactc gcctttttca gctttggcgc gcgcaccttc cacgtgatct
     7321 aaaatccatt taattttggg gccagagaaa taggttgcca gcggaagtcc cgtcttggaa
     7381 cgcagtctgt cctgcccgcc caccttggcg agttcgttgc aaatgacatc cgtccgcgta
     7441 tcctgccaga caatggcatt gtacacaggc ttccccgtgg atttatccca caccactgcc
     7501 gtttcacgct gattggtgat tccaatggcg gcgatatctg cggcttgaat tccgctcttt
     7561 tgcaaggcgc ccttcatgac ttcctgtgtg cgttcccaaa cctcaagcgg atcgtgctcc
     7621 acccagcctg gtttcgggta aatttgacga tgctctttct ggtcaacagc aataacattc
     7681 cccccgtgat cgaagatgat aaagcgggta ctggtggtac cctgatctat ggcagcaaca
     7741 tattttgaca tggcatcctt ccttggcgtt aattgtaaaa caaaaccacg aaaaaatgac
     7801 catgtatcag aaacacaaat ttctctataa agacatcaag ccgcaaagaa aaaatcttcg
     7861 cggctgggtt ttcaggcatt ccctgcccca cacttcaaca gactttcatc tttaaatcaa
     7921 aacggcggtt gaaacccgcc gttttactta attctctgta atttccacaa agcgcaaatc
     7981 tggattcttc tcgatgaagt acttcaggaa aaacggggtg ctgaaaatcg ccgcccattc
     8041 ctcccgcgaa tcgcgcgcaa tgatgacttc gctgcgttgg gtgatatttt ggatcgccgt
     8101 ttcaggtcct ttgatccagc gcatcatcac atgcggcaga cgttccaact cggtgggcac
     8161 attgtattcc gctttcaaac gggcctgcac cacatcgaac tgcaattgac cgaccactgc
     8221 cagaatcggc tcacgtttga aggcattgac attaaacaac acctgcacgg ccccttcgct
     8281 ttccaattgc aagagtccct tttggaactg cttctgcttg cccaggtctg tgttgcgaag
     8341 cagtgcaaaa tgctcaggtt gaaaacgagg gatcggggca aaccgaacca gctcccccgt
     8401 acagagagaa tcgccgatgg caaattctcc gttgttggga atcccaagca catctccggg
     8461 gtaggcttcg tcaatgatct cgcgttcgct ggcaaacagt ttatacggac gaaccagccg
     8521 cacggtcttg ccgctctgcg catggaccag gtccatgccg cgctcaaagc gacccgagca
     8581 aatacgcagg aaggccacac tgtcgcggtg ctttgggttc atattcgcct gtattttgaa
     8641 gatgaaacca gaaaagccag ggtcttgcgg cacaaccgct cccctatcgc tcaagtatgg
     8701 ctggggttga ggcgcgtatt tcacaaaggt atcgaggaag aggcgcacgc caaagttggt
     8761 gaccgcactg ccaaagaaga ccggggtttg cttttcctgc aatacaaggt catggtcgaa
     8821 aaccacaccc acttcatcca gcaaagcgac gctttccacc agttcgtcat agcgtttctg
     8881 cggcaggata ttgttctttc caagttcatc cagcggaacg aatatctcag gcgccatgcg
     8941 ttcattgcgt tcggtgcgct cgaacaaata cacacctttc gaggcacggt catacacgcc
     9001 gcggaattcg gggccgtcgc ctatgggcca gttcattggc acaggttcca tgcccaacac
     9061 ttttttgatc tcatccagca attcaagcgg atcacgggcc gggcgatcca ttttgttgat
     9121 gaaggtaaaa atcggaatcc cacgcttgcg gcagacttca aacaatttca gcgtttgcgg
     9181 ttcgatgcct ttggcggcat cgatcaccat cacagcactg tccaccgccg aaagggtgcg
     9241 gtaggtatct tcagaaaaat cctgatgtcc gggggtatcc agcaggttga caatattgct
     9301 ttggtacgga aactgcaaaa tggtcgaagt gatcgaaata ccgcgctcgc gctccatctc
     9361 catccaatcg gaggtggtcg aacgttggtt cttgcgcgca cggacattgc ccgccagttc
     9421 gatggcattg ccatacaaaa gcagtttttc ggtcaaggtg gtcttgcccg catcaggatg
     9481 ggaaataatg gcaaaggtcc gccgcgcagc gatcggatcg tccattgaag tgttttgggg
     9541 cggctttgca gccagtaaat catcttgcat gctttaatct ccgggaaatt acaaaggccg
     9601 tagattttag catagatttt tatcaatcgg aaaaaannnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnctga cgtttactac ccgccatccc cccagttttg cggattttac
     9781 tcttcgctct tgcgggagcg ggatttcttc acaggagctt cttcttcaac aaccgcaggt
     9841 acttcttcca caacttcttc ttcatcctct tcaatttcat cggaactggt gaagaagtta
     9901 taaaccaaca cagccagggt tgcaccgatc aacggaccaa acagatagat cagataggtg
     9961 tttgggctgg tgaggctcgg aatggaagca aagacagccg gtcccagggt gcgggcaggg
    10021 ttgagtgaag cgccggtgaa aggtccaccg cccaaaatgg ccagagccaa tgtggtgccg
    10081 atcgcccaac cagcaaagcg tccgcccttg ccgccaacgg cagtgtgcaa gatcgtgttg
    10141 acgaggaaga aggttaggat cgcttccatc accatcgcca aaatgggagc cttatcggtg
    10201 agcgcaccgg tggtcgcgcc agcatcaatg gggccaccca aagaatcgag gaacaatttc
    10261 atcacaaatg cagccaggat cgcgcccgca aactgcgcaa tccaataaac cacagcctga
    10321 ccccaggtga caacattttt cagtgcgagt gcaaaggtca cagcggggtt cacgtgcgtt
    10381 ccggaaatat ggccgtaggc gtaagcgaaa accgccagcg taaatccatg tgcaagagcc
    10441 acacccaaca ggcctgcatt cacaatgcct gcacccatgc cgataaacac cagcgcgaat
    10501 gtgccgatga attcggcaag aaacactttc gaattgaaca ttttcttctc caaaaagata
    10561 tgatttgaga ttataacccg tggggtctcg atacgctacg caactgcaac ccaatcccgt
    10621 gcgcctgcca gaaaggattt gcccggcatt gccttttttc ctgcaggctg aatttcatcc
    10681 aaaaccaagg cgccagcgga agtttgaaca gcgggtcttc cttcgaccgt aaacctgctg
    10741 ccgcttgcca agcctttttc gccgctgaca gtggatgcgc gcaacacctt gagcggattc
    10801 ccattccact caaaccatgc gcccggccag ggattcatcg cccgcacacg gcgttccagg
    10861 tcaatggcgg gttgggtgaa atcaagcagg ccgtcggtct ttttcaacat cggcgcatac
    10921 gtgaatccat cctcgggttg cggcgtggcg gtgagattcc ctgcaaggta ggcaggcagg
    10981 gtctcgatga gcaggtctgc tcccagtgtg gataatgcct gtgaaaccga acccgctgta
    11041 tcgtcaggtt tgatgcgcac ggacctcttt gccagcatgg ggcccgtgtc gagtcccaca
    11101 tccatttgca tgatggtcac gcctgtttct tcatccccgt gtaaaatggc ggcgttgatg
    11161 ggcgccgcgc cgcgccaacg cggaagcagg gaagcatgca cattgataca gccatgacgg
    11221 ggcaaatcca atacgtcttt tttcagaatc tgaccaaaag ccgcaaccac aatgagatct
    11281 ggctcccatg cacgtaactg ttccatggct tcgggctggc gcagcttttc aggctgcata
    11341 acaggcaggt tcagttcaag cgcaagggat ttgaccgggg gtggtttcaa ctcacggcca
    11401 cgccctgagg cgcggtctgg ctgggtgacc acaccaacca cttgataatt cggttcggcg
    11461 gccagtgtgg aaagagaagg caatgcaaaa tcgggcgaac ccatgaaaac aattcgtaat
    11521 gtcatccttt gattttaccg cttgttgaat ttttttcagg atctgcaaca tggtttgcgc
    11581 tttaaaattc aatccagtac tcttgggtga agaggattgg ttgcatgata gtgggaatgg
    11641 ttgtaaaata ctggaaatcc aaaatcaact tattcggagg aataaatcat gtctcaagca
    11701 ccaatgtctc ctgatgttac cagtgacgat aa
//
LOCUS       DAOQLC010000129        11626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2141_length_11626_cov_21.417423, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000129 DAOQLC010000000
VERSION     DAOQLC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2141_length_11626_cov_21.417423"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..551
                     /locus_tag="PL000_17585"
     CDS             21..551
                     /locus_tag="PL000_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90746.1"
                     /translation="MTIILETPRLILRHLVLDDLDALFALYRDPEIRKYFPDGVKNYE
                     DTREELEWYLNGHPEHPELGLWATVHKETGKFIGRCGLLPWEIDSRLEIEVAYLLDKK
                     FWHQGLATEAAKGILAYAFDNLSLKRVICLMHPDNIASQRVAQRVGMILEGSTDNVAA
                     NNFPTLIYAINKPVAS"
     gene            604..1101
                     /locus_tag="PL000_17590"
     CDS             604..1101
                     /locus_tag="PL000_17590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90747.1"
                     /translation="MDITIRRLEPSDYEAMQKIFAGPKVVWGTLQLPYPSIEIWRNRI
                     AEVPNDMFRLVAIVGTEMAGQLDLFTSPNHPRRRHAGQIGMSVRDDFQGQGVGSALMQ
                     VATDLADNWLNLQRLELEVYCDNEPAVGLYKKFGFEIEGTLKGYAFRAGEFVDVYAMA
                     RTRLK"
     gene            1147..1638
                     /locus_tag="PL000_17595"
     CDS             1147..1638
                     /locus_tag="PL000_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90748.1"
                     /translation="MLVRWLVFIHVLSAITFFLAHGAAAAMVFKVRSETDFARIRAML
                     DLSVSTFKAYMLSFFMMGVTGLTMPFLIHIWNRAWIWLSIVLILFVVVWMGLVNEKQI
                     KQLRRLVGLPYMQGFNEFPAETPASPEEVAALLKKINPYQWALVGYGIPAVVLWLMVF
                     KPF"
     gene            1679..2176
                     /locus_tag="PL000_17600"
     CDS             1679..2176
                     /locus_tag="PL000_17600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90749.1"
                     /translation="MDIVIRRLEPSDYEAMQKVFAGPKVTWGTLQQPFPSVEIWRKRL
                     AEIPESQFWLAAMVGNELAGQLDIQTSPNHPRRKHVGQLGMAVRDDFQGKGVGTALMR
                     AAVDMADNWLDLLRLELGVFVDNEPAVRLYQRFGFFIEGTLTRYAFRAGTYADVYAMA
                     RFKPG"
     gene            2277..3890
                     /locus_tag="PL000_17605"
     CDS             2277..3890
                     /locus_tag="PL000_17605"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC90750.1"
                     /translation="MSKRIFISYSRREIGFVDALVDQLESKGNQVWLDYRSLIPGTPW
                     LDQIHRGIEEADVLLLVVSKASLASQNVEVEWKSVLQQKKRIILLVFEAVDLPNELEK
                     YEWVDFRGSFQRGLNELFSQMAKPVNENHPVPQTGFKMPGIVWVAFLLSVVVAILSLP
                     TIWTLLIPWVLIPLPYRILKRNYNFTYVQAALFFLPIALFSGTIINDNINAAEFDVIM
                     ANTVLGFPLAIILLFVLRSSGMQRWGKEEATLPRFANPYTPDNPRPSPVPFYIDHAPQ
                     DARIAEMLENTLEDYGHPVVKQMQDARAVFVMLSRFKKDSEADPEKQVVFPVIVQTSE
                     DISPKLAKVQWIDMRSGVRGLDAIAQLLPEPEKLLKALGNRPRGRMLVLPAPVAAMQF
                     FLTLLGVFAMGCFFKLFVELINNDVFNLPNGDSFMVLVFFFIADLVLVGWLLVTMLRN
                     LIQREGGLASFRAFTRALGGVGFLAFLQTALGIMLDSSFADIFGEDSPIIGIVSIFPT
                     FIFLVGGVVMAVFLLVRYKDIRAWFPVSK"
     gene            4162..>10042
                     /locus_tag="PL000_17610"
     CDS             4162..>10042
                     /locus_tag="PL000_17610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNC90751.1"
                     /translation="MKNPSIKYIVITVLLVIFVVASLVVNQRTGTSTVSSETQEGETS
                     SYIPQSEPVRVDAPSQPVPQQVVIQFAPDATEAERAAYIESIGGTVVESIDSLDTIVV
                     NVPEEVAQAPLPESTAVASSEADYYVAALDEVAPVNDPHYVDQWALPAIAAPEAWAAM
                     SADAPAVTIAVIDSGICVTHPDLNGRIVSGWDFVEGDAVPQDDFGHGCAVSGVIAANM
                     NDGIGIAGVAPNANIMPLRVLNSSGFGSYSDVAAAVVFAVDNGAQVINLSLGGSSPSS
                     TLENAINYAVANGVVVVAAAGNNGTEGALYPAAYDSVVAVGSIDPNLAHSSFSNYGSQ
                     VDVWAPGRDIMTTKRTGDYGLVSGTSFAAPYVAGAEAVYMALSRTPVFAGGLLTFSTQ
                     IEVTAEPTTVVEVTPTDVPVTEISDAAVLEQLRSIYNLPADRDAFVQTIQQAGGTLFG
                     LFIIPAPDNAEGGPSAHYFTAVQNGDSLTISQEYTPKFAEALDALAAEGVVDQGVVDT
                     FNSPTAQGDGTAQLGLPWTTGANATFVAGPHGDGFQPFPTEIANALDFAPADNKVRAA
                     REGFAQQIVCPGQVQSSWVIVTHPGGWRTSYYQVSNIQVDPDGLSVGRGAYLGDLAAN
                     SASALRCGGNNFTSPLVHFSVLQNGKQVKINGFDIGGWTVGTTTMTKVSDASVVNVGG
                     TLSNNGTYGTNLTCDATVLPPDYIKCADEGGTCSFPGLETVYFGSDGCFGTEAATTSI
                     SCDSGTFGMPAGDSRSCYVDSKSTTLSPACPQSGGVILYWAKDFSCTNAASDAGYRLQ
                     AASGSQDYGVDTAFNDKASALRVPTGWSVMLYGGAANSGEMLCVNKDVSDLSLLGTFP
                     NSASEVNDGISSMQVFANTNCGGVLNSYAPPQNLLPLNGSSNANNFDLNFQWAAAPMG
                     GSYYVEWWNNNTPNTKNFCNWSTALNCDIAGTPAAGTYSWHVRAFDGVDYSGWSQTFT
                     FTIRTSANAIFLDRLAITDELGPAGLGDGSPSPAEYVEANAYKDIFLPGDPIRLYIYY
                     TNQYPTNEAIDFEWLVTDPAGRIVPEMSETAVDTSNNANNSWYWHRAYAIPDDAIRGT
                     YTFTGKVTYNSVTTTQSQTFTITGSPSVEVHDAFSTTSLGVASVDGGPGVPEASIPSD
                     VISKTTPTYNAGDNIRLYIDSFNDTASAVNATFTWQVLDPIGRHVPQLEWAGTLSAAV
                     GHTWWSLPATIPSYMGTGDYVFTGTINNGGHITSQSQTFHVNGLSAPANDNWANRITI
                     GSATYTNTQDTWNATSENDDPSSSCGGTNSNSVWYRYTPASNGLLEIDTTGTDYPVEI
                     GVWTGASLASLTQFACSNELDIVTGDVAIKNMSVTSGTTYYIELTDVGTPGGGKLKLS
                     VNFISSASLTNDGINNAATITNNITINNAGANITPLDVRSATVGVEDRALDSCNRAIG
                     KATVWYKYITTLPGDLVLDTGGSNYDTMLSVWEDVPGSGLSLISCNDDIGGGVWDVDS
                     HLLAQLKASTTYYIQVAEFNGVSENSNSLSLDGIGNKPPVSAEFDGGTLHLFSQFNPA
                     PILDLPADNTYTDDTTPTLSLSTTLLPSGAATYHFQVDDSSDFSSALVNTAGVAGTSY
                     TVTAGEALPSGVYYWRVAAQDGSANYSAWSASRTLNITSLISPTNNSVTSNTKPTFSW
                     GASAGALEYNLQIDESTSFAAVELDRLQAGTSFTLTTALVPGDHYWRVRVRTASGWGG
                     FTPPWKFTILQALVAPVLENPAASFATTDRTPSFSWFSVPAGNTYNIQIDTDATFATP
                     DVDYTTLPGNPISYQQPTDLPDGTYSWRVRALNVLGEFGPWSTARTFTIDNVASDAPL
                     LNLPADNAYTALSKPTFSVFAVTGAASYTIYVDTEPFTNPAKVPFVFQTGITTTSYTL
                     NDAQVLPYGVYYWRMRAKDAAGNNSSWSVIRTLYVTPMTAPANNGFVTTATPNFTWVA
                     AT"
     assembly_gap    10043..10142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10143..>11626
                     /locus_tag="PL000_17615"
     CDS             <10143..>11626
                     /locus_tag="PL000_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90752.1"
                     /translation="NNGFVTTATPNFTWVAATGALEYNLQVAKTADFASIEMERLQTS
                     GTSYTSPALDYGSHYWRIRVRTATGWGSWAPAWKFTVSPALTAAPVLVSPAVSAVVAD
                     NTPTLTWNKVMDDDRYEIQIDNLSTFASPEQTSLTSFNVLNYTATTLPDGIYYWRVRN
                     TNYLDVGGTWSTSRSFTVDTTPPAAPNLSAPADAASNIGTPAFSWAAPTTATKYQFEY
                     DNNSDFSSPTYTSAELTTTSHTPSPAMALGLHYWHVRARDAAGNWSSWSASRSITINP
                     PIPLTVTVLSPAASAVTNDNTPDFTWNALGVGTPYTYTYEIQFATASTFAAPLPQSAT
                     GLAVTNYTPASLFTDGVIYWRVRGVNVNNVTGPWSTSRSFTVDTIAPLAPSLTAPADA
                     AAVTTSPAFTWAAATTASKYQFAYDSANTFDSNGGNALYTSAELTTTSHTPPSMLTPP
                     LPKTYYWHVRAKDAAGNWSPWSVTRSLQINPATPLAPTLTLPAAS"
BASE COUNT         2592 a         2991 c         2962 g         2981 t
ORIGIN      
        1 aattggcgtt gggataaccc atgacgatca tcctcgaaac cccgcggctt atcctacgtc
       61 atctcgtcct ggatgatctc gacgcattat ttgccttata tcgtgacccc gaaatccgca
      121 agtattttcc tgatggcgtg aaaaattatg aagacaccag ggaagagctc gaatggtatc
      181 tgaatgggca tcctgaacac cccgagttgg ggttatgggc gacggttcac aaggagactg
      241 gtaaattcat tggtcgctgc ggcttgctgc cgtgggaaat cgactccaga ctggaaatag
      301 aggttgctta tttattggat aagaaattct ggcatcaagg tttggcgaca gaagctgcca
      361 aaggaatttt ggcatatgcg ttcgacaact taagtctgaa gcgagtcatc tgcctgatgc
      421 atcctgacaa tatcgcctca caaagagttg cccaacgagt cggcatgatc cttgaaggaa
      481 gcactgataa tgtagccgca aataattttc cgaccctaat ttatgcgatc aataagccag
      541 ttgcttcatg acaaggcgat ccaactggca ttgggtttaa aaaaccagat aggaattttg
      601 aatatggaca ttaccatccg ccgtttggaa ccaagtgatt acgaagccat gcaaaagatc
      661 ttcgcgggtc ccaaagtagt ttggggaacg ctacaacttc cctacccttc catcgagatc
      721 tggcgcaacc gcatcgctga ggtacccaac gacatgttcc gactcgttgc catagtggga
      781 accgagatgg cagggcaatt ggatctattc acctcaccca atcatccccg ccgcagacac
      841 gcggggcaga tcggcatgtc cgtgcgggat gactttcaag gccagggcgt cggctcggcg
      901 ctgatgcaag tcgccacaga cctggcggac aactggctca atctccaacg cctcgaactg
      961 gaagtgtatt gcgataacga gcccgcggtg ggactctaca agaaattcgg cttcgaaatc
     1021 gaaggcacgc tgaaaggcta cgcgttccgc gcaggagaat ttgtcgacgt gtacgcgatg
     1081 gcaaggacaa ggttgaagta acggttacat tcgattcata caggtatttg taaaggagaa
     1141 aatcttatgt tggtccgctg gctcgtgttc attcatgttt tatcggcaat cacgttcttt
     1201 cttgcgcatg gggctgccgc ggcgatggtg ttcaaagtcc gctctgagac ggactttgcc
     1261 cgcatccgtg ccatgctgga tttatccgta tccaccttca aagcctatat gctgtcattc
     1321 tttatgatgg gggtgacagg gcttaccatg ccattcttaa tccacatctg gaatagagcg
     1381 tggatctggc tgtcgatcgt tttgattttg tttgttgtgg tctggatggg tctggtcaac
     1441 gaaaaacaga tcaaacaact gcgccgttta gtggggctgc cctacatgca ggggtttaat
     1501 gagttcccag ctgaaacgcc tgccagccct gaagaagttg cagctttgct caagaagatc
     1561 aatccatatc aatgggcact ggtcgggtat gggattcccg ccgttgtatt atggttgatg
     1621 gtcttcaagc ctttctaagc catttgatat ttgttgaata aaaaggagac ctgtcctcat
     1681 ggatatcgtc attcgcagat tggagccttc tgattatgaa gccatgcaaa aagtatttgc
     1741 cgggcccaag gtgacctggg gtacgcttca acaaccattt ccttccgttg aaatctggcg
     1801 caaacgtttg gctgaaatac cggagtctca attctggctg gcggcgatgg tcggcaatga
     1861 actggcgggg cagttggata tccaaacctc acccaatcat ccacgccgca aacatgtggg
     1921 gcagcttggc atggcggtgc gcgatgattt tcaaggaaag ggtgtgggta cggcccttat
     1981 gcgggctgct gtggatatgg cagataactg gcttgatctg ctgcgccttg agctgggagt
     2041 ctttgtcgat aacgaacccg ctgtccgctt gtatcagaga ttcgggtttt tcatcgaagg
     2101 gacattgact cgttatgcct tccgtgccgg tacttatgca gatgtctatg ccatggctag
     2161 gttcaagcca ggatagtttc attgtgttgc aagtgtttta caaattcaga aactgggaga
     2221 agagcatctt caaaagggta taatttttat gcattcttta ttgtgtgagg tgtcttatga
     2281 gtaaaagaat atttatcagc tattcacggc gtgaaatcgg tttcgtcgat gctcttgtag
     2341 atcaattgga gagcaaaggg aatcaggtct ggctggatta tcggagcctg attcctggta
     2401 ccccctggct tgaccagatc cataggggaa ttgaagaagc agatgtactt ttgctggtcg
     2461 tgtccaaggc ttcgctggct tcacaaaatg ttgaagtgga gtggaagagc gttctccagc
     2521 agaagaaacg catcatcctg ctggtgtttg aggcggtgga tcttccaaat gaacttgaaa
     2581 aatatgagtg ggtggatttt cgcggtagtt ttcagagggg attgaatgaa ttgttttctc
     2641 aaatggcaaa gcctgttaat gaaaatcatc ctgtcccgca aactggattc aaaatgcctg
     2701 gtattgtttg ggtggctttt ctattaagtg tagtggttgc catcctttcc ctgcctacca
     2761 tatggaccct gcttatccca tgggtgttaa ttcccctgcc gtatagaatt cttaagcgca
     2821 actataactt tacatatgtt caggcggcgt tgtttttcct gccgattgct ttattctccg
     2881 gtacgatcat taatgacaat attaatgcgg ccgaatttga tgtgattatg gcaaataccg
     2941 tgcttgggtt tccgctggct atcatccttc tatttgtact gcgttcttca ggaatgcagc
     3001 ggtggggtaa agaggaggcc accctgcctc gctttgccaa cccatacaca ccggataatc
     3061 cacgtccgtc ccccgtccct ttttatattg accatgcgcc acaggatgca aggatcgctg
     3121 agatgttgga aaacacgttg gaggattacg ggcacccagt tgtgaagcaa atgcaggatg
     3181 ccagggcagt ttttgtgatg ctctcccgct tcaagaaaga cagtgaagcg gatcctgaaa
     3241 aacaggttgt gtttcctgtc attgtgcaga cctcggagga tatttcgccc aagcttgcca
     3301 aggtgcagtg gattgacatg cgcagtggag tgagaggact ggacgcgatt gcgcagttgt
     3361 tacccgaacc tgaaaaactg ctgaaggcac tgggcaaccg tcccaggggc agaatgttgg
     3421 tgttgccagc gccggtggca gccatgcaat tctttttaac cctgctgggt gtctttgcca
     3481 tggggtgttt tttcaagctg tttgttgagt tgattaacaa cgatgtgttc aacctgccaa
     3541 atggggattc tttcatggtc ctggtgttct ttttcattgc ggaccttgta ttggtcggct
     3601 ggttgctggt taccatgtta cggaatttga tccaacggga aggcgggctg gcaagttttc
     3661 gtgcgtttac acgagccctg ggtggggtgg gatttctggc tttcctgcaa acagctttgg
     3721 gaatcatgct tgatagctct ttcgctgata tttttgggga agattctcct attatcggta
     3781 ttgtctccat ttttccgact tttatctttc tagttggcgg ggttgtcatg gcagtgtttt
     3841 tgttggtacg atacaaggac atccgtgcat ggtttcctgt atcaaagtaa tggactgggg
     3901 gattgggacg agacgaggtt tacttcttat ggatgagaat gaggcggttc ggctgcattc
     3961 actttacccg taatgaatcc acccctgtac ttgtctgaca tagtaccaat ggactagtga
     4021 tttctttaac catttgtaag tgatttataa ggcaaatggg agtttaagaa gctaaaattg
     4081 aaataaaccg acgataggcc cgctttcagg cgggtgatga tgtttgtgtg actgatcgat
     4141 gatgataaaa ggaagtgtaa tatgaaaaat ccctccataa aatacattgt gattacggtg
     4201 ctgctggtga tttttgtcgt ggcatccctg gtggtgaacc agcgtactgg aacctccacg
     4261 gtctcttcag agacgcagga aggagaaact tcctcataca ttccacaatc agagcctgtg
     4321 cgtgtggatg cgccctcaca gcctgttccc caacaggtgg tgattcagtt tgcgcccgac
     4381 gccaccgaag ctgagcgtgc tgcttacatt gaatccattg gcggtacggt tgtggaatcg
     4441 atcgattcac tcgacaccat tgtggtcaat gtgcctgaag aagtggctca ggctcctttg
     4501 cctgaatcca cagcggtggc atccagtgaa gcggattatt atgttgccgc tttggatgag
     4561 gttgcacctg tgaatgaccc tcattatgtg gatcagtggg ctctgccggc cattgccgca
     4621 cccgaagcat gggctgccat gtctgcggat gcgcctgcgg tgacgattgc tgtgatcgat
     4681 agcggtatct gtgtcaccca ccctgatttg aacggccgca ttgtcagcgg ttgggacttt
     4741 gtggaaggcg atgctgtgcc gcaggatgat ttcggtcatg gctgtgcggt gagcggcgtg
     4801 atcgctgcga acatgaacga tggtattggt attgcgggtg ttgcgcccaa tgcgaatatc
     4861 atgccattgc gtgtgttgaa cagcagcggt ttcggttctt attcggatgt ggctgcagcg
     4921 gttgtatttg ctgtggataa cggcgcgcag gtcattaact tgagccttgg tggttcaagc
     4981 ccttcgagca cgctggaaaa tgccattaac tatgctgttg cgaacggcgt agtggtggtg
     5041 gctgcggctg gaaacaacgg cacggagggt gccttgtacc cggctgctta tgattctgta
     5101 gtggcggtcg gttccattga cccgaatctg gctcacagtt cttttagtaa ttacggctcc
     5161 caggtggatg tgtgggcccc gggccgtgac atcatgacca ccaaacgcac cggtgactat
     5221 ggcttggtga gtggtacttc ctttgctgca ccttatgtgg ctggcgccga agccgtgtac
     5281 atggctttga gccgcactcc tgtcttcgct gggggactgc ttactttttc gacccagatc
     5341 gaggtcacgg ctgaaccgac gactgtcgtt gaggtcactc ctactgatgt gccggttaca
     5401 gagattagcg atgcggcagt gcttgagcag cttcgttcca tctataacct gcccgcagac
     5461 cgtgatgctt tcgtgcaaac cattcaacag gctgggggaa ccttgtttgg cctgttcatc
     5521 atccctgccc cggacaatgc cgagggtgga ccttctgctc attactttac ggctgtacag
     5581 aacggtgaca gcctgaccat ttcacaggaa tacacaccga aatttgctga agcactcgat
     5641 gcattggctg cagaaggtgt ggtggatcag ggtgtggtgg atactttcaa cagcccaacc
     5701 gcccagggtg atggcacagc tcagttgggc ctgccatgga caaccggtgc gaacgccacc
     5761 tttgtggcgg ggccgcatgg ggatggcttc cagcctttcc cgaccgagat cgccaatgcg
     5821 ctcgactttg cgcctgctga taacaaggtg cgcgctgccc gagaaggttt tgcccaacag
     5881 atcgtctgcc cgggtcaggt gcagagcagt tgggtgattg tgactcaccc cggcggctgg
     5941 cgtaccagtt actatcaggt ctccaatatt caggtggacc ccgacgggtt gagcgtggga
     6001 cgcggtgcct acctcggtga cctggctgcg aacagcgctt cagctttgcg ttgtggcggc
     6061 aataacttta cttccccact ggttcatttc tcagtgttgc agaacggcaa gcaggtcaag
     6121 atcaatggat ttgatatcgg cggttggacc gtgggcacaa ccaccatgac caaagtgagc
     6181 gatgcatccg tggtcaatgt gggtggcaca ctttcgaaca atgggactta cggtacgaac
     6241 ctgacttgtg acgcgaccgt acttccccca gattacatca aatgtgccga cgaaggcgga
     6301 acctgtagtt tccccggcct tgaaacggtc tattttggtt cagatggctg ttttggtact
     6361 gaggctgcca ccaccagcat ttcctgcgat agcggaacct ttggcatgcc tgccggtgat
     6421 tctcgttcct gttatgtgga tagcaaatcc actacgttgt caccggcttg tccgcaatcg
     6481 ggcggagtga ttttatactg ggccaaggat ttctcctgca ccaacgccgc atctgatgcg
     6541 gggtatcgcc tgcaagctgc cagcggatca caggattatg gtgtggatac tgccttcaac
     6601 gataaggctt ctgctttgcg tgttccaacg gggtggtctg tcatgcttta tggcggtgca
     6661 gcgaatagcg gcgaaatgtt gtgcgtgaac aaggatgtca gcgatctttc cctgcttggc
     6721 accttcccca actccgcttc ggaggtcaat gacggaattt cctccatgca ggtgtttgca
     6781 aatacaaatt gtggcggcgt cttaaattcg tatgcacctc cacaaaacct gcttccgttg
     6841 aacggctcca gtaatgccaa taactttgat cttaatttcc aatgggctgc tgcaccaatg
     6901 ggtgggtcct actatgtgga atggtggaac aacaataccc ccaacaccaa gaacttctgc
     6961 aactggtcca ctgcccttaa ttgtgatatt gccggtaccc cggcggcagg aacttactcc
     7021 tggcatgtac gggcttttga tggtgtcgat tattcaggtt ggagtcagac attcacattt
     7081 accatccgta ccagtgcaaa tgcgattttc cttgatcgtc ttgcaattac agatgaattg
     7141 ggaccggcag gccttggcga tggcagcccg tctcctgctg aatatgttga ggcgaatgcc
     7201 tacaaggata tattcctgcc cggtgatcct attcggctat acatatatta caccaaccaa
     7261 tacccaacca acgaagcaat tgatttcgaa tggttggtca cagacccggc tgggcgtatt
     7321 gtgccagaaa tgtctgaaac cgctgtggac acctcgaata atgcgaataa cagttggtac
     7381 tggcatcgtg cctatgccat cccagatgac gctattcgtg gaacatacac tttcactgga
     7441 aaggtcacct acaatagcgt aacgaccacc cagtcgcaga ctttcacgat tacaggcagc
     7501 ccttccgttg aagtgcatga tgccttctcc accaccagcc tcggtgtggc ttcggtggac
     7561 ggcggccccg gggttcctga ggcttccata ccttcagatg tcatcagcaa gaccacaccc
     7621 acttacaatg cgggcgataa tatccgcttg tacattgatt ccttcaacga tacagcctct
     7681 gctgtcaatg ccacttttac ctggcaggtt ctcgacccga tcggacgtca cgttccgcaa
     7741 ctggagtggg ccggcacatt gagcgcggca gtggggcata cctggtggtc tctgcctgcc
     7801 accattcctt cgtatatggg gacgggtgat tatgtcttta ccggcaccat taacaacggt
     7861 ggtcatatca ccagccagag ccagaccttc catgtcaatg gcttatcggc tccggcaaac
     7921 gataattggg ccaatcgcat caccattggc agcgccacct acacaaatac acaggatacc
     7981 tggaacgcca cctcggaaaa tgacgatccc tcttcgtcct gcggcggtac caactcaaac
     8041 agtgtttggt atcgctatac cccggccagc aatggtcttt tggaaatcga caccaccggc
     8101 actgactacc ccgttgagat cggcgtgtgg actggcgctt ctcttgccag ccttacacag
     8161 tttgcctgct ccaacgaact ggatattgtc accggtgatg tggcgattaa gaatatgtct
     8221 gttaccagcg gcaccaccta ttacatcgag ttgaccgatg tgggtacccc aggcggcggc
     8281 aaactgaagt tgagcgtcaa cttcatcagc tctgcttccc tcacaaatga tggcatcaac
     8341 aatgccgcta ccatcaccaa caacatcacc atcaataacg ccggtgcaaa catcacccca
     8401 ttggatgtcc gctctgctac ggtgggtgtg gaggaccgcg cactggattc ctgtaaccgc
     8461 gccattggaa aagccacagt ttggtacaag tacatcacca ccctgccggg cgatcttgta
     8521 cttgatacag gtggcagcaa ttacgacacc atgctttccg tctgggaaga tgtacccggc
     8581 agtggtctct cccttattag ctgtaacgac gatatcggcg gcggggtatg ggatgtcgac
     8641 tcgcatcttt tggcgcagtt gaaagcctcc accacctact atattcaagt tgctgaattc
     8701 aacggcgtat ctgaaaactc aaacagcctg tcactcgatg gcatcgggaa caagcccccg
     8761 gtctccgcgg aattcgacgg cggcaccctg cacttgttct cgcagtttaa ccctgcaccc
     8821 atccttgatt tgcctgctga taacacctac accgatgaca ccacgccgac cttgagcctt
     8881 tccactacat tgctccccag tggggcagcc acctatcact tccaggtgga tgatagctcg
     8941 gacttttctt ctgccctggt gaacactgcc ggtgtcgctg gcaccagcta caccgttacc
     9001 gcaggtgaag cgttaccgtc aggtgtttat tattggcgtg tcgctgcaca agatggcagc
     9061 gcaaactatt ctgcctggag cgcatcccgc acattgaata ttacgtcact gatcagcccg
     9121 acgaataatt cagtgacaag caacaccaaa cccaccttca gttggggagc gtctgcaggt
     9181 gcgcttgaat acaacctgca gattgatgaa tcaacctcct ttgccgctgt ggaattggat
     9241 cgcctccaag ccggtacctc tttcacgctc actacagccc ttgtccctgg tgaccattat
     9301 tggcgtgtcc gcgttcgtac ggcttcaggc tggggcggat tcaccccacc atggaagttc
     9361 accattttgc aggcattggt tgcaccggta ttggaaaacc cggctgcatc tttcgccacc
     9421 accgaccgta caccttcgtt ctcctggttc agcgtgccag ccgggaatac ttacaacatt
     9481 cagatcgata ctgatgccac ctttgccacc cccgatgtgg attacaccac actccccggc
     9541 aaccccatca gttatcaaca gcccactgac ctgcccgatg gaacctactc ctggcgtgta
     9601 agagccctca atgttcttgg cgagtttggt ccgtggagta cggctcgtac cttcacgatt
     9661 gataatgttg catccgatgc tccgctcttg aatctgcccg cagataatgc ctatactgcg
     9721 ctttccaaac cgacctttag tgtttttgca gtaactggtg ctgcttcgta tacaatttat
     9781 gtagatacag agccgtttac aaatccggcg aaagtcccat ttgtatttca gacaggtatt
     9841 accactacaa gctatacatt aaatgatgca caagtattgc cctatggggt gtattactgg
     9901 cgtatgcgtg caaaagatgc agctggaaat aactcctctt ggagcgtgat tcgcacactg
     9961 tatgtcaccc ccatgacggc cccggctaat aacggtttcg tcaccacggc cacacctaac
    10021 tttacatggg ttgctgctac agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnaataacgg tttcgtcacc acggccacac ctaactttac atgggttgct gctacagggg
    10201 cgcttgaata taaccttcaa gtagccaaaa ctgctgattt cgcttccatt gaaatggagc
    10261 gcttgcaaac cagcggtaca tcctacacca gtcccgcatt ggattatggt tcacattact
    10321 ggcgtatacg tgttcgcaca gcgactggat ggggttcatg ggctcccgcc tggaagttca
    10381 cggtctcacc tgcgctgaca gctgctcctg ttctcgtcag cccggccgta tctgctgttg
    10441 ttgcagacaa tacccccacc ctgacctgga acaaggtgat ggatgatgac cggtatgaga
    10501 ttcagatcga taatctcagc acctttgcat caccggagca aacctctctt acgagtttca
    10561 atgttcttaa ctatacagct acaactcttc cggatggaat ctactactgg cgcgtacgca
    10621 acaccaacta tcttgatgta ggaggtacat ggagcacttc ccgctccttt acagtggata
    10681 ccaccccgcc cgctgcgccg aatctctctg caccggcaga tgccgcttcg aatattggca
    10741 caccggcctt ttcatgggcg gcgccaacca cggcgaccaa ataccaattt gagtacgata
    10801 ataattccga tttttcgagc ccgacctaca catcagcgga attgactacg accagccata
    10861 ccccctcgcc agccatggca ttgggattgc attactggca tgtccgtgcc agagatgccg
    10921 ccggtaactg gagttcatgg agtgcatctc gcagcattac gatcaaccca cccatcccct
    10981 taaccgtaac ggtgctctcc cctgctgcat cagcggtaac aaacgataac acgcctgact
    11041 tcacgtggaa tgccctgggt gtcggaacgc catacaccta tacctatgaa atccagtttg
    11101 ccacggcgtc cacatttgcg gcaccgctgc cacaatcagc gacgggtttg gcggtaacaa
    11161 attacacccc cgcctctctg ttcactgatg gtgttatcta ttggcgtgtt cgtggtgtga
    11221 atgttaataa cgtcaccggt ccgtggagca catcccgttc cttcactgtg gatacgatcg
    11281 ccccgcttgc accaagcctg accgccccgg ccgatgcggc agcagtcacc acttcaccag
    11341 ccttcacgtg ggcggctgcc actacagcaa gcaagtatca atttgcgtat gacagtgcca
    11401 acacctttga ctccaatggc ggcaatgctt tatacacttc cgctgagttg accaccacca
    11461 gccatacccc gccatccatg ctaaccccgc cactgccaaa gacctactat tggcatgttc
    11521 gcgcaaagga tgcggctgga aattggagcc cgtggtcagt gacgcgttcg ctccagatca
    11581 accctgccac tccgcttgct ccgaccttga cattgccagc ggcttc
//
LOCUS       DAOQLC010000130        11247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2242_length_11247_cov_26.956934, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000130 DAOQLC010000000
VERSION     DAOQLC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2242_length_11247_cov_26.956934"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PL000_17620"
     CDS             complement(<1..1427)
                     /locus_tag="PL000_17620"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HNC90753.1"
                     /translation="MKNKLLQTILSSLAIGFAVLACGQFGATPVPQSPTGVAIATIVP
                     EETLEAVAGSAGLGDSLYPGFGNGGYDVTHYLLDFTIHDVATSDLTAIVTIEANATQD
                     LGSFNLDFIGFDINSLTVNGAEADYSRNGQELIVSPLKPLSDGDAFTVVVQYSGSPEE
                     MQSVALPVQTGWVTVEGGSFVLSEPDGSANFYPVNDHPLDKAAYTFRVTVPKPFEVAA
                     NGLLIETTDKGDATTFVFDSPSLMASYLATINIDEFETETMESDGGIPIRNYYSAGLS
                     EKVRAPFARQGEMIDYFSELFGPYPFEVYGSLVMDVEFGTALENQTMSIYGIDMIDLE
                     DVEGTELTVAHELAHQWFGDSVSVADWRDIWLNEGFATYAEVLWVEHADGPEARDEYV
                     SDLYSDMELYGDVGGAPGDPPADDLFNASVYEWGGLTLHALRLEVGDEAFFEILRTYH
                     ERYKNSNATTEDFVAVAEEISGQELG"
     gene            1499..2143
                     /locus_tag="PL000_17625"
     CDS             1499..2143
                     /locus_tag="PL000_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HNC90754.1"
                     /translation="MNASPLFEFALLGFISMFTMINPIGVIPVYTAMTAKLSPQESHR
                     VARKASLTALFILLAFALTGQLIFRFFSISVNSLRVVGGVIFFLMGYEMLQAHLSRTQ
                     FDDETTHEYINDISITPLGIPIICGPGAITTAILLMNESSSPLESGIVIGIIIAIITF
                     TYVLLLGAKQVTHLIGENGNKVMLRLMGLIVMVIAVEFFFSGLTPILREIFKIQ"
     gene            2151..3698
                     /locus_tag="PL000_17630"
     CDS             2151..3698
                     /locus_tag="PL000_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90755.1"
                     /translation="MNRKLDWPTLVLFAALLFGAIVRFWPALTNGFPLNDGGMFYTMI
                     LDLKENNYQLPEFTEYNNADIPFAYPPFGFYIAALLSDLLPVSALQILLWLPALVNTL
                     SIFFFYKLAGQVLNSRLLASLAVLIYALSSRAFLWQVMGGGITRAFGMLFLILMLWQA
                     VQLFKSEGHPTHLALTILFGAGAILSHPQTALHAVLGGAILFGFYGRNKRGVLSVTAI
                     GLGVVLLTAPWWGTVLLRHGFEPLLSAGQTSQRTLESYLAILDFSGLDDYVFLPAFLL
                     AVVGAVAMFKKHEFLLIAWPLLALLADPRGGEGIALLPLSMLAGTGLEKAANWLNRSK
                     EGSSEDPLSNRKAQALLFVLFTYLILGAMIFDFQLVNTSLKPADLEMIQWVKENINER
                     KTFLLSTGREFSMSDPMQEWFPALTSQYSVTTLQGLEWTLNELFFPWHSELVAFQHCA
                     DLNCVTNWSGKNKINYDYLLVTVPASEDKSILADSLRNLALSAKASPSYTVVYESDST
                     LIFEFHR"
     gene            complement(3733..4389)
                     /locus_tag="PL000_17635"
     CDS             complement(3733..4389)
                     /locus_tag="PL000_17635"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNC90756.1"
                     /translation="MNQSHIIQKTAEYIKQEFSEDSSGHDWWHIYRVWKNAITICEHE
                     KADPFIVELAALLHDLDDWKFNDTGDETPLRAKAWLDSCGVEIPIADEVCEIIKHISY
                     KGAGVENKMKSLEGFIVQDADRLDAIGAIGIGRAFAYGGYKNRPMYDPESAPQMHATF
                     EQYKNSKSATINHFYEKLLLLKDMMNTPTAKKIAEQRHEVMLGFLDQFMKEWEGRDVF
                     "
     gene            complement(4433..4792)
                     /locus_tag="PL000_17640"
     CDS             complement(4433..4792)
                     /locus_tag="PL000_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90757.1"
                     /translation="MRWVILILVTLTAGWMLADGLRALTIGDYFTPASGEYAGQLGPW
                     TLFVQALGIEPRSTFMKAIFVVYGIAALIAVAGFALNQPWGRNALIAVALLGLWYLPI
                     GTAANIVVLILLFLKRS"
     gene            complement(4794..5267)
                     /locus_tag="PL000_17645"
     CDS             complement(4794..5267)
                     /locus_tag="PL000_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90758.1"
                     /translation="MSQSITHEDFFNNLMALLDETFDNTHGIYLDKDTSLFRTLETVS
                     AEEASIPVGGKCASLAAQVAHVTFYMEVLERYVVHHDNSRVDWGEVWRTVEKVTPEEW
                     TASKNKLQATYQRISNLFRENPVWNDDSIGGSLAIVVHTAYHLGEIRQALCVLKK"
     gene            complement(5269..5838)
                     /locus_tag="PL000_17650"
     CDS             complement(5269..5838)
                     /locus_tag="PL000_17650"
                     /inference="COORDINATES: protein motif:HMM:NF039496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HNC90759.1"
                     /translation="MSSLDKAVQTQLENIQKKTGKSLAELAAIAQKSGLSKHGELRDM
                     FKEKLGLGHGDANALVHAIFQSDGTRAAEGKNEDAVLDEIYSGAKAGFRPIHEKLIKE
                     MKKFGEFEIVPKKGYVSLRRKKQFAMIGPKTNTRFEVGINAKDFKQNARLLEQPKGSM
                     CNYIVNVNDPREVDAELVAWLRSAFDGAG"
     gene            complement(5859..6398)
                     /locus_tag="PL000_17655"
     CDS             complement(5859..6398)
                     /locus_tag="PL000_17655"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNC90760.1"
                     /translation="MAYQFTSMFHLVSAVSALAFGACVLYTRKGTRLHKQLGYAYLLN
                     MLGLNITALFIYQLTGHFGPFHGAALASLLTLLAGFVPAYLRLPHGRWLEFHYEFMNW
                     SYVGLIAAGVSEVATRLPSAPFWPAVVASMFAVFLFGGIWISRSRGKHLSNARNSAIR
                     SDAPVNGWTALRRRRTACQ"
     gene            complement(6435..6842)
                     /locus_tag="PL000_17660"
     CDS             complement(6435..6842)
                     /locus_tag="PL000_17660"
                     /inference="COORDINATES: protein motif:HMM:NF020157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HNC90761.1"
                     /translation="MENLYINHAAVFVCAIFNLALGALWYSPALFYKAWLAESKLTEE
                     QLAKQNPAKTYGLAFLFSLLISYNLAFFLGDANTNAAWGAAAGFLAGFGFAALIFAVI
                     ALFEQRSWKYVFVNGGFITVYFTVIGFILGIWR"
     gene            complement(6914..7741)
                     /locus_tag="PL000_17665"
     CDS             complement(6914..7741)
                     /locus_tag="PL000_17665"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC90762.1"
                     /translation="MRFEIHHPSFPLSNFIDSLIYFEGMRPQHSLDRFLPDGNTELII
                     ELSDAPQYIHHNDTLEIVQSCRYAWVSGVRTHPITIPSGRESTKLVVTFKKGMARAFY
                     PFPMHELTDSVVMADLVFGRRILDFRERLLFTRSPQEMFQLVELFLLNMAGDKLYEDT
                     KVTSVVYAVTSMFRQPTLLNFKKMGEQIGYSQKHFIELFKGQVGVPPKQYLKIARFQK
                     AIKAIEGNRPVQWSDIAMDSGFYDQAHFINDFKAFSGFTPGEYIKRKVPTLNYVPVA"
     gene            7878..8699
                     /locus_tag="PL000_17670"
     CDS             7878..8699
                     /locus_tag="PL000_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNC90763.1"
                     /translation="MATKTKKTTPVFDSDEEDIIEEAPALIPAPTIQDSSEETITFKK
                     SHFYSVLVVLAFAVGVLVGYVVWGPGSGRSTATAAAAQAAAQAPAGAEQAPQYRRYDI
                     PTEGFPSLGPEDAPIVIVEFSDFQCPFCRRFHDETYQSLLEAYPGQIRFVYRNLPLTS
                     IHPAAMPSAIASLCAKDQNAYWDYHEKLFSSEQLDEETFIRYAADLGLDTEKFTSCLA
                     SGEHDAEIQEDMSFAIDLGVQSTPTFFVNGLAIVGAQPLANFQQLIDKELAGEIP"
     gene            8830..9495
                     /locus_tag="PL000_17675"
     CDS             8830..9495
                     /locus_tag="PL000_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC90764.1"
                     /translation="MKKFVAIAGNIGVGKSTLVKMLCNEMGWEPFYEPVTENPYLADF
                     YQNMSAWAFHSQVFFLTHRLRSHMGLAQHPSSVIQDRSVYEDAEIFAQNLYDQGNMQD
                     RDYQTYRELYETAVQFLPPPDLVIYLRASVPTLLNRINSRGRAYERTIAPEYLQNLND
                     LYESWIENFTLCPVLAVPADDLDYVAHSGHLRLIIAKVEDKLVGKEEVVFEPEEVARA
                     AED"
     gene            complement(9524..10420)
                     /locus_tag="PL000_17680"
     CDS             complement(9524..10420)
                     /locus_tag="PL000_17680"
                     /inference="COORDINATES: protein motif:HMM:NF016533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HNC90765.1"
                     /translation="MQLPETFISTIKNVYKRDGERFLAVLPVLIDEVSDRWGLTKVTP
                     VPNLSFNFVAFANRGDDEVILKMGVPNPELTSEMTALKLFNGDGACQLLEHDEERGLL
                     LLERLKPGKMLAKLEDDEERTIIAAEVMSKLWREVSSDNKFIKLSDWFAGLKKIRPHF
                     DGGTGPFPKKLLERVESFLPELFADTDVKLMHGDFHHFNILSSERGWLVIDPKGVIGP
                     VGYEVGPLMLNPWSSISDWSRFKVHAERRVSILAERLGWEREKIINWSTAHAILSAWW
                     SIEDNMEDANTIQCAEIFSQLK"
     gene            complement(10471..>11247)
                     /locus_tag="PL000_17685"
     CDS             complement(10471..>11247)
                     /locus_tag="PL000_17685"
                     /inference="COORDINATES: protein motif:HMM:NF025986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC90766.1"
                     /translation="SIGVGMYQHDVDQTALTHALDGVVESVVNSVGVDVNTASSALLT
                     HVAGIGPKLANNIVTHRDANGPFKSRTALKKVAGLGPKAFEQAAGFMRIRGGANPLDE
                     SAIHPESYAIAESILARAALTVDSSIPDRIAALESLTAKISHEELAKELNCGVPTLKD
                     ILEQLVRPGRDPRSDTPAPILRSDVLKAEDLLTGMQLKGTVRNVVDFGAFIDIGVKQD
                     GLLHRTQIPNGTVLKVGDIIDVEIQKIETERGRISLGWVK"
BASE COUNT         2716 a         2985 c         2720 g         2826 t
ORIGIN      
        1 cccaattcct gtccgctgat ctcctcggcc acagcgacaa aatcttcggt ggtggcgttg
       61 ctgttcttgt agcgctcgtg ataggttctc agaatttcga agaaggcttc atcgcccact
      121 tcaaggcgca gggcatgcaa tgtgagtccg ccccattcat aaacgctggc attgaacagg
      181 tcatccgctg gaggatcgcc cggcgcgccg cccacatcgc catacaattc catgtctgaa
      241 taaagatcgc tgacatactc atcccgcgcc tcggggccat cggcgtgttc cacccataag
      301 acttcggcgt aggtggcaaa cccttcgttg agccagatat cgcgccaatc agccacactg
      361 acactgtcgc cgaaccactg gtgcgccagt tcatgtgcca cagttaattc tgtgccttcc
      421 acatcttcga ggtcgatcat atcaatgcca taaatggaca tggtctggtt ttcgagcgcc
      481 gttccaaatt caacatccat aacaagggag ccatatacct caaagggata cgggccaaat
      541 aattcgctga aataatcgat catctcaccc tgccgggcaa acggtgcgcg gaccttttca
      601 ctcaaccccg ccgagtaata attgcggatc ggaatcccgc catccgattc catcgtctcg
      661 gtctcaaatt cgtcaatatt gatcgtggcc agataacttg ccatcaaact gggtgaatcg
      721 aagacaaatg tggttgcgtc acctttatcg gtggtctcga tcaacaggcc attggcggcc
      781 acttcaaatg gttttggaac cgtgacacgg aaagtatagg cagccttatc caatggatgg
      841 tcattgaccg ggtaaaaatt tgccgagcca tcgggttcac tcaacacaaa actgccgccc
      901 tccacggtaa cccaacccgt ttgcacaggc aatgcaacag attgcatctc ttccggtgaa
      961 ccgctgtatt gaacgaccac ggtaaatgca tcaccatccg aaagcggttt gagcgggctg
     1021 acgatcagtt cctgcccatt gcgggagtag tctgcttcag ccccgttgac cgtaaggctg
     1081 ttgatatcaa agccgataaa atcaaggttg aaactgccga ggtcctgggt ggcattcgcc
     1141 tcaatggtga cgatcgcggt caggtcgctg gtggcaacat catggatcgt aaaatccaac
     1201 aggtagtgcg tcacatcata gccaccgttc ccaaaccccg ggtagagcga atcgcccagg
     1261 cctgccgaac cggcaaccgc ttccagcgtc tcttcaggga cgatggtggc aattgccaca
     1321 ccggtcggtg attgtggaac aggggtggcg ccaaactggc cgcatgcaag aacggcaaaa
     1381 ccgatcgcaa gagatgagag aattgtttga agtaatttat ttttcatttt tatccttgtg
     1441 gttgaggcag tatatcacgg tggtgtgttg taaaattgaa tgcacagatt ggagtcaaat
     1501 gaacgcttcc ccgctttttg aatttgcatt gcttggcttt atttccatgt tcaccatgat
     1561 caaccccatt ggcgtcattc ccgtttacac ggcgatgacc gcaaaattat cgccgcaaga
     1621 atcgcatcgt gtggcgcgca aagccagcct gacggcgtta ttcatcctgc tggccttcgc
     1681 cctgacgggg caactgatct tccgtttctt ctccatctcg gtcaacagcc tgcgcgtggt
     1741 gggtggcgtg atcttttttt tgatggggta cgaaatgctg caagcccacc tttcacgcac
     1801 acaatttgac gatgaaacca cccacgaata catcaacgac atctccatca cgccgcttgg
     1861 catccccatc atctgcggac cgggtgcgat cactaccgcc attttgttga tgaacgaatc
     1921 ctcttcaccg ctcgaatcag ggatcgtgat cgggatcatt attgcgatca tcaccttcac
     1981 ctacgtcctg ctgcttggcg caaaacaggt cacacatctc atcggcgaga acggcaacaa
     2041 ggtgatgctg cggctgatgg gtttgatcgt gatggtcatt gccgtggaat ttttcttcag
     2101 cggtctgacc ccgatcctcc gcgaaatttt caagatacag tgaggctcac atgaatcgca
     2161 aactggattg gcccaccctc gtgctttttg ccgcgctgct ctttggcgca atcgtccgct
     2221 tttggcctgc gctcacaaat ggttttccgc ttaacgatgg cggcatgttc tacaccatga
     2281 tcctcgattt gaaggaaaac aattaccaac tgcccgaatt cacagaatac aacaatgcgg
     2341 atatcccctt cgcctacccg ccattcggat tttacatcgc cgcgcttctt tctgacctgc
     2401 tgcctgtttc tgcgctgcaa atattgctct ggctgccagc tttggtgaac accctctcga
     2461 tcttcttctt ttacaaactc gccggacaag tcctcaattc acgtctactg gcgtcactgg
     2521 ctgtgttgat ctatgcactt tcgtcacgtg cctttttgtg gcaggtaatg ggcgggggca
     2581 tcacccgcgc ctttggcatg ttgtttttaa ttctgatgct atggcaggcg gtgcaattgt
     2641 tcaagagtga ggggcaccca actcatcttg cgttgaccat cctgtttggt gcgggcgcga
     2701 tcctgagtca cccgcaaacc gcattacatg cagtgctcgg cggtgcaata cttttcgggt
     2761 tttacgggcg caacaaacgc ggagtgcttt ctgtaacggc cattgggttg ggcgtggtgc
     2821 ttctcaccgc tccctggtgg ggaacggtgc ttttgcgcca cggctttgaa ccgttactct
     2881 cggcggggca aacgagtcag cgcacactcg aatcgtatct tgcgattttg gatttcagcg
     2941 gcctggatga ttatgtcttc ctgcctgctt ttctgctggc ggtggtcggt gctgtggcca
     3001 tgttcaaaaa acatgagttc ctgctaatcg cctggcctct gctggccctg ctggcagacc
     3061 cacgcggcgg cgagggcatc gccctgttgc cattgtccat gctggctggg accgggttgg
     3121 agaaggccgc caactggcta aaccgttcaa aagaagggtc aagcgaagat ccgctctcga
     3181 accgcaaggc tcaagccttg ttatttgtcc tttttactta tctcattctc ggcgccatga
     3241 tctttgattt tcaactggtg aataccagtt tgaagcccgc cgatcttgaa atgatccaat
     3301 gggtgaagga aaacatcaac gagcggaaga ccttcctgct ttccacaggg cgcgaatttt
     3361 ccatgtctga ccccatgcag gaatggttcc ctgcgttgac gagccagtac agcgtcacca
     3421 ccttgcaggg ccttgagtgg accttgaacg agcttttctt cccgtggcac tcagaactgg
     3481 tcgcatttca gcattgcgcc gatctaaatt gcgtcaccaa ctggagcgga aagaacaaaa
     3541 tcaactacga ctatctgctg gtaactgtcc ctgccagcga agacaaaagc attttggctg
     3601 actcgctgag aaaccttgcg ctttcagcaa aggcatctcc atcctacacg gtcgtatatg
     3661 agtcggacag cacattgata tttgaattcc accgatgacg atattttcag ggaacagaat
     3721 ccagattgcc agttagaaaa catccctgcc ttcccattcc ttcataaatt ggtcgagaaa
     3781 gccaagcatg acctcatgcc gctgttctgc tatttttttg gcagtcggtg tattcatcat
     3841 gtcttttagc aaaagcaatt tctcgtaaaa gtgattaatg gtggcgcttt tactgttctt
     3901 gtactgttca aaagtggcat gcatttgcgg cgcagactca ggatcgtaca tgggtctgtt
     3961 cttgtagccg ccataggcaa aggctcggcc aataccgatg gcgccgatgg catcaaggcg
     4021 gtcggcatcc tgcacgatga agccttcgag gcttttcatt ttattttcca cacccgcccc
     4081 cttgtaggaa atgtgtttga tgatctcgca aacttcgtcg gcgatgggaa tttccacgcc
     4141 gcaagaatca agccaggctt tggcgcgaag cggggtttca tcgcctgtat cgttgaattt
     4201 ccagtcatca agatcgtgca gcagggcggc cagttccacg atgaatggat ccgctttttc
     4261 gtgttcgcag atggtgatgg cattcttcca cacgcgatag atgtgccacc aatcgtgtcc
     4321 ggaagagtcc tcggagaatt cctgtttgat gtattcagct gttttttgga tgatgtgaga
     4381 ttggttcatg cggctatttt gccacagaaa tcaggcatta cgtggtgcgg ggtcaactcc
     4441 ttttgaggaa taacaaaatc aggacgacga tattggccgc cgtgccgatg ggcagatacc
     4501 acaagccgag aagcgcaact gcgatcaatg cgttgcgccc ccagggttga ttcaatgcaa
     4561 agcccgccac agcaatcagc gccgcaatgc cataaacgac gaagatggct ttcatgaatg
     4621 tggagcgcgg ctcgatgcca agtgcctgca caaaaagtgt ccacggacca agctggcccg
     4681 catattcgcc gctggcgggt gtgaagtaat caccgatcgt caatgcgcgc aggccatctg
     4741 caagcatcca gccagcggtc aaggtgacga gaatgaggat gacccatctc atattatttt
     4801 ttcaacacac acaatgcctg gcggatctcg ccgaggtgat acgccgtgtg aaccacaatg
     4861 gcaagcgagc cgccgatcga gtcatcgttc cacacggggt tttcgcgaaa caaattcgaa
     4921 atgcgttgat acgtggcctg caatttattt tttgaggcgg tccattcttc aggcgtcacc
     4981 ttttccacgg tgcgccacac ttcaccccaa tccacgcggc tgttatcgtg gtgaaccaca
     5041 taccgctcaa gcacttccat gtagaaggtt acatgggcca cctgtgcggc gagcgaggcg
     5101 cactttccgc ccacagggat cgatgcctct tccgcagaga cagtttcaag ggtccgaaaa
     5161 agagacgtgt ctttatcgag gtagataccg tgggtgttgt cgaaggtttc atcaagcagt
     5221 gccatcaggt tgttgaaaaa atcttcgtga gtgatggatt gtgacatttt atcctgcccc
     5281 atcaaatgcc gacctgagcc acgcgacaag ttctgcatcc acctcacgcg gatcattgac
     5341 attaacgatg tagttgcaca tgcttccttt tggttgttcc aacagacggg cattttgctt
     5401 gaagtccttc gcgtttatgc ccacctcaaa acgggtgttg gtctttggac cgatcatggc
     5461 aaactgtttt ttgcggcgca ggctgacata tcccttttta ggaacgatct cgaactcgcc
     5521 gaactttttc atctctttta tcaatttctc gtgaatgggt cgaaaacctg ccttcgcacc
     5581 tgaataaatt tcatcgagga cggcgtcttc gtttttacct tccgcagcgc gcgtgccatc
     5641 agactgaaaa atggcatgga caagcgcgtt ggcatcgccg tgacccagcc caagcttttc
     5701 cttgaacatg tcgcggagtt cgccatgttt gctcaatccg cttttttggg caatggcggc
     5761 aagttctgca agcgacttgc ctgtcttctt ttggatattt tccaattggg tctgtacagc
     5821 tttatcgagg ctactcataa atcatctcct taaaaaaatc attgacaggc tgtgcgcctc
     5881 cgccttaagg cagtccaccc attcacaggc gcatcagatc gaattgccga gttccgcgca
     5941 ttggacaaat gttttccccg gcttcgggaa atccatattc ctccgaacaa aaagacagcg
     6001 aacatactgg cgaccaccgc aggccagaac ggtgcggaag gcagacgggt ggcaacttct
     6061 gatactcccg ccgcgatcaa ccccacatac gaccagttca tgaattcata gtgaaattcc
     6121 agccaacgcc catgaggaag gcgaaggtat gcagggacga atccagccag caaggtgagt
     6181 aaactcgcca atgccgcacc atgaaaaggt ccgaaatgac ccgtgagttg ataaatgaaa
     6241 agtgcagtga tgttgagtcc cagcatgttt aagaggtacg catatcccag ttgtttgtgc
     6301 aggcgtgtcc ctttgcgggt atacagtaca cacgcaccaa aggccaatgc tgagacggca
     6361 gacaccagat gaaacattga tgtaaattga tatgccattt tcttccttct aaagagaaaa
     6421 catcctatta ttttctaacg ccatataccg agtataaatc cgatgaccgt aaaatacact
     6481 gtgataaagc caccgttgac gaaaacatat ttccacgagc gctgctcgaa cagggcgatg
     6541 actgcaaaaa tcagggcggc aaaaccgaaa cccgcaagga aacccgccgc cgctccccag
     6601 gctgcattcg tgtttgcatc tccaaggaaa aatgccaggt tatacgagat caacagcgaa
     6661 aacagaaagg caagtccata ggttttggcg gggttttgct ttgcaagttg ttcctcggtc
     6721 agttttgatt cagccagcca ggctttgtaa aacagagcgg gcgaatacca tagggcgccc
     6781 aatgccagat tgaaaatagc gcaaacaaaa acagcggcgt gattaatata caggttttcc
     6841 atgagatttc tcctttgttt tttggaggta atcttacaga aatcgaatcc aaatgtattg
     6901 gaaaaaattt acattaagcc accggaacgt aattcagagt cggcaccttt cttttgatgt
     6961 attcgccggg agtgaacccg gaaaaagcct tgaagtcgtt gataaagtgt gcctgatcgt
     7021 aaaaaccgct gtccatggca atatcactcc attggacggg ccgatttcct tctattgcct
     7081 tgatcgcttt ttgaaaacga gcgatcttga gatattgttt tggcggcaca ccgacctgcc
     7141 ctttgaacaa ttctatgaaa tgtttttggg aatacccgat ctgctccccc atttttttga
     7201 aattgagcaa agtgggttgg cgaaacatgc tggtgaccgc atatacaaca ctggtaacct
     7261 ttgtgtcttc gtaaagttta tccccggcca tgttcagcag aaataattca accagttgga
     7321 acatctcctg cggtgatctg gtaaaaagca atctctcacg aaaatcaaga atgcgcctgc
     7381 caaaaaccag atcagccatg acgaccgaat cggtcagttc atgcatcggg aacggataaa
     7441 aagcacgggc cattcccttt ttgaaagtca ctaccaattt ggtgctctcc ctgcccgaag
     7501 gaattgtgat tgggtgggtc cgcacgccac tgacccaggc ataacgacag gattgaacga
     7561 tttccaacgt gtcattatga tgaatatact ggggtgcatc tgacaactca atgataagtt
     7621 ccgtgtttcc atcgggcaga aatctatcca aagaatgctg tggacgcatg ccttcaaaat
     7681 agatcaggct gtcaataaaa ttactcaacg gaaatgatgg gtggtgaatt tcaaaacgca
     7741 tgacgaattt tccaatacca gtattttaga acaaatattc tgttattgca agcccgtttt
     7801 tagaacttca acagagtaaa tcttagccaa acctaagtcc ttttccacaa aaatcttgta
     7861 agggctataa ttccctcatg gctactaaaa ctaaaaaaac cactcctgtt tttgattctg
     7921 acgaagaaga catcatcgaa gaagctcctg ccctgattcc cgcccccacc atacaggatt
     7981 cttccgaaga gaccatcact ttcaagaaaa gtcattttta ctcggtactg gttgtgctgg
     8041 catttgcggt cggcgtgttg gtagggtacg ttgtttgggg tcccggctct ggtagaagca
     8101 ccgctacagc cgcagctgct caggccgctg cgcaagctcc tgcaggtgcc gaacaggctc
     8161 cgcaatacag acgctacgat atccccactg aaggcttccc cagccttggc ccggaggatg
     8221 cgcccattgt gatcgttgaa ttcagcgatt tccaatgccc attctgccgc cgcttccatg
     8281 atgagaccta ccaatccctg ttggaagcct accccggcca gatccgcttc gtgtaccgca
     8341 acctgccgct tacctccata cacccggcgg ccatgccctc cgcgatcgcc tcgctctgtg
     8401 ccaaagacca gaatgcctac tgggattatc acgaaaaact gttcagcagc gaacaactcg
     8461 atgaagagac cttcatccgc tacgctgccg atcttgggct ggataccgag aaattcacct
     8521 cctgccttgc aagcggcgaa catgacgccg aaattcaaga ggatatgagc tttgccattg
     8581 accttggcgt acaatccaca ccgacgtttt tcgtcaacgg gcttgccatt gtcggcgccc
     8641 agccgctcgc caacttccaa caactgatcg acaaggaact ggccggggag atcccgtaac
     8701 cgcaagccag gatcgatttc aaataaagag ttcacggaag ttcttaaaat ttccgtgaac
     8761 tctttttctt tttggtatga tgtcccgcgt gcctatcacc cccaaagatt ttttatttga
     8821 gagcagaaca tgaaaaaatt cgtagccatc gctggaaaca tcggtgtggg aaaatccacg
     8881 cttgtaaaaa tgctatgcaa cgaaatgggc tgggaaccgt tctacgaacc cgtcactgaa
     8941 aatccatatc ttgcagattt ttaccagaac atgtctgcct gggcttttca ttcgcaggta
     9001 ttcttcctca cccatcgact gcgatcacat atggggcttg cccaacaccc cagttctgtg
     9061 attcaagacc gcagcgtgta tgaagatgct gaaatcttcg cgcaaaacct gtacgaccag
     9121 ggcaacatgc aagaccgcga ttatcaaacc tatcgcgagt tgtacgaaac cgccgtgcaa
     9181 ttcctgcccc caccagacct tgtcatttac ctgcgtgcct ccgtgcccac gctgcttaac
     9241 cgcatcaact cacgcggacg cgcctacgag cgcaccatcg cccccgaata tctgcagaac
     9301 ctcaacgacc tctacgaatc atggatcgaa aatttcacac tctgccccgt gctggctgtt
     9361 ccagcagatg accttgatta tgtagcccac tccggtcatc tgcggttgat catcgcaaaa
     9421 gtggaagata aactggtcgg aaaagaagaa gtggtcttcg agcctgaaga agttgcacgc
     9481 gccgcagaag attagatcat aaaaaacctc ccgagggagg tttttatttt agttgggaaa
     9541 atatctcagc gcattgaatc gtgttggcat cttccatatt gtcttcgatg ctccaccatg
     9601 cagacaggat ggcatgagcc gttgaccagt tgatgatctt ttcgcgctcc caacccaacc
     9661 tttcggcaag gatgctcacc cgcctctcag cgtggacctt gaacctgctc cagtccgaaa
     9721 tgctgctcca cgggttgagc ataagcggac ctacctcata cccaacaggc ccaatcactc
     9781 ccttcggatc aatgaccagc caaccacgtt ccgatgaaag aatattgaag tgatgaaagt
     9841 cgccgtgcat taatttcaca tcggtgtcag caaataactc gggcaaaaat gattccactc
     9901 gctccaaaag tttttttggg aatggccccg tcccgccatc aaagtgcgga cgtatcttct
     9961 tcaacccagc aaaccaatct gataatttaa taaatttatt gtcacttgaa acttcgcgcc
    10021 atagcttact cattacctca gcggcgatga tcgtgcgttc ttcatcatct tcgagcttgg
    10081 caagcatctt gccaggcttg agtctttcca acaataaaag cccgcgctct tcgtcatgtt
    10141 cgagcaattg acaggcaccg tcaccattga ataatttcag tgcggtcatt tcgctggtca
    10201 actcagggtt tgggactccc attttgagaa tgacttcgtc atcacctcga tttgcaaaag
    10261 cgacaaagtt aaaagataaa ttgggaacag gagttacctt cgttaatccc caacggtctg
    10321 agacctcatc tattaaaaca ggaaggacag cgagaaatct ctcgccgtcc cttttgtaga
    10381 cgttttttat ggtggaaatg aaggtttcgg ggagttgcat tttatctatc gtactccata
    10441 gggacacagc ggcgctgtgt ccctacaaga ctatttcacc cagccaaggc taatgcgtcc
    10501 gcgctcggtt tctatttttt gaatttcaac atcgatgatg tcacccactt tgaggacggt
    10561 gccgttgggg atttgcgtgc ggtgcagcaa accatcctgt ttgacgccga tgtcgatgaa
    10621 ggcgccgaag tccaccacat tgcggacggt gcctttgagc tgcatccctg tcagcaggtc
    10681 ttcggctttg agcacatccg agcgcaggat gggggcggga gtatcggagc gcgggtcacg
    10741 gcctgggcgg acgagttgtt cgaggatatc tttcaatgtg ggaacgccgc agtttaattc
    10801 tttggctaac tcttcgtgag agatttttgc ggtgagggat tccaaggcgg cgattctgtc
    10861 tgggatggac gaatcaactg tcagggcggc acgagcgaga atggattctg cgatcgcgta
    10921 ggattctgga tggatggctg attcatccaa cgggttcgct ccgcctcgaa tccgcatgaa
    10981 gcctgctgcc tgttcaaagg cttttggccc aagccctgcc accttcttca atgcggtacg
    11041 ggatttgaag gggccgttcg cgtcgcggtg tgtgacgatg ttgtttgcga gtttggggcc
    11101 gatgcccgca acatgcgtga gcagcgcgga gctggcggtg ttgacatcca cgccgacgct
    11161 gttgaccacg ctttcgacaa cgccatcgag ggcatgcgtg agcgcggttt gatcgacatc
    11221 gtgctgatac atgcccacgc caatgga
//
LOCUS       DAOQLC010000131        11132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2278_length_11132_cov_23.977340, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000131 DAOQLC010000000
VERSION     DAOQLC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2278_length_11132_cov_23.977340"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1406
                     /locus_tag="PL000_17690"
     CDS             117..1406
                     /locus_tag="PL000_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90767.1"
                     /translation="MNQKVIIFFAIILLAACSSSPSRVSTESPSVEPSQTSIPSPLPL
                     PSDTPEPSPTVTPIPIGGGQLLVAFYASGSCGACIIIGDFFTGEILFEIPLSTDFQTG
                     NIFWSPDGESILYSDITTARMNVLLFDLETGQSKKLGDHPRKGGSPEFVNSMKYVEWS
                     YDSEYVMYDVYYEDSNLQKSYFASKSGVPGFYESGFTDWFPDSRTMFSIYGGKDSYNV
                     ETNTFVPTSTDVLSKFNVVYILKDFILLEREKTKISVIPFPENWNDALAWQYDALGSQ
                     VVVLAQLSEEIKNGQVHVYVADKIDDNKVSVIGSVLVSNQMSYFVKIIDLKNLPVEIK
                     PDDLLQQIGDTPLVVSPDGDYYLKGYCTFMETCLNYNPNWKNEIVRGWGFQVVGFNGV
                     EQVLPADLSQFKGVTKTTANVINTSDGLLDGIAFYWK"
     gene            1512..2003
                     /locus_tag="PL000_17695"
     CDS             1512..2003
                     /locus_tag="PL000_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90768.1"
                     /translation="MKTLGGVIMKDYWAILNYEIQMYFGARHLQSFPINGASQSLTTL
                     QASAMTEVKALHVRILLDIFLERKNSDDINIDDILPDWREKNQTVVHNLEIAYNKKLE
                     IGKTPKWYLNKYLAHPDKRRGDHFDWAPVIKRMDTPLKEVFKTLPTEKLHALVFFQKY
                     LYA"
     gene            2193..2510
                     /locus_tag="PL000_17700"
     CDS             2193..2510
                     /locus_tag="PL000_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90769.1"
                     /translation="MENEYAEWIYFGISLVIAAWIIVAINSSAKNTLIGYLRERGAKD
                     IVVIREWLSFDRTITFFVEFTHPDGNKISTYCKFGRLGTFEDGEIFWSVPTNLEPPKP
                     KTK"
     gene            2868..3119
                     /locus_tag="PL000_17705"
     CDS             2868..3119
                     /locus_tag="PL000_17705"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNC90770.1"
                     /translation="MTLQQITIEIPEKVLLAEKTDAESFGREILILAAVKLFEIGRLS
                     SGRASELAGMSRIEFLLNLNRYKVFPFASELDDLENAHA"
     gene            3112..3597
                     /locus_tag="PL000_17710"
     CDS             3112..3597
                     /locus_tag="PL000_17710"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HNC90771.1"
                     /translation="MPKAISNTSPLLYLYKIGAINLLPQIFDEVWTAESVKNELLAGR
                     SKGYDVPNPADYPWLNIVNPKSMPSEWLALDLGAGELGAMALALENPDRIVLLDDMLA
                     RRTAQVAGLQVWGTLKVLLEAKSQGLIEKIEPYISKLSDAGMWVSAEVKQRILILAGE
                     S"
     gene            3723..4265
                     /locus_tag="PL000_17715"
     CDS             3723..4265
                     /locus_tag="PL000_17715"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC90772.1"
                     /translation="MKKTPVATRKPKSSSTVVKAELRRDALIADLIETRRKIVDVASS
                     LSPTEQDQVFLGIWSMKDLLAHLIGWLEENRKAVKTIRAGKLPEFYAYKDRDWQTYNA
                     YLVSKYKRGDMTELLAKLQVSQQKFIKSIRAVPIEVLEQDFGVRFNRYKVTIVRLLQA
                     ELKDEKVHYAQMEAFRNKAK"
     gene            4375..4863
                     /locus_tag="PL000_17720"
     CDS             4375..4863
                     /locus_tag="PL000_17720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90773.1"
                     /translation="MEIHIRKATANDYDDLCELFDEVDALHRVNLPHVFQKSTGPARE
                     KDYYLGLIADENIGLFVAEMEEKLAGFVHVLVRDTPVFSIVIPRCYAIVDGIAVKSEF
                     QKHGIGKMLMDKAQEWAETKDAEFMELNVYEFNRNAISFYEGLGYQSSSRKMRKEISK
                     AG"
     gene            4982..5884
                     /locus_tag="PL000_17725"
     CDS             4982..5884
                     /locus_tag="PL000_17725"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HNC90774.1"
                     /translation="MPENVSTYQIVDLMPDFWEFHAKNRDADVSEQASQFKRMVVDKH
                     PEAYAARVIGLDPNKPYDEALAERYLLCHERSTAQQVSTMQRISASIARDMSAYEEKF
                     RKVFPDFNYTGSVYFMNSLLGFDGATRTVNGKIALLFGLDTMAWVYGDDLDLQPFFHH
                     EIFHIYHSQFLEDDSDEVAAAVWQEGLAQFVAKSLNPDAEGVNLFGLPKEMPQRSEAM
                     LSTLASGLLGVLDSTSRDDYGRYFFGNNGSEEIPPRSGYYLSYLVVQKLSKKYSLQEM
                     AHSKLAAIKPEIKTALEELANGGR"
     gene            5887..6711
                     /locus_tag="PL000_17730"
     CDS             5887..6711
                     /locus_tag="PL000_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90775.1"
                     /translation="MQGETMEAYDDDLGRFEAEGAAPLPDSGNQGYIDHDNARIWYAD
                     YGVGPTVVLLHGGLGHSGNWGYQIPTLIGNGYRTIVIDSRGHGRSTRDDRPYTYELMA
                     SDVLAVMDVLGVEKAALAGWSDGACIALVLAAQVPARVEGVFFFACNMDPSGAKEITE
                     FAPILQRCIGRHIKDYGQLSETPDQFEALSEAVGLMQRTQPNYSANDLAQITVPVAVI
                     HSEFDEFIKLEHAEYLARSIPNADFVYLSGVSHFAPLQRPDQFNDTLLNFLGKVLV"
     gene            6884..7597
                     /locus_tag="PL000_17735"
     CDS             6884..7597
                     /locus_tag="PL000_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90776.1"
                     /translation="MTDKPSTTQPIMDRRLLVLLVLSVAFLVIAFLGGQPSSARGFYY
                     AVWLTFSGWLWKKRERLSLWLQARPLPEFPLFVGLGLVMILVEETIAGVAVHLLNVDS
                     VGQLFAVVPLYYFNNLLLLPGFIVGWYVLLKFCAYSRTEVFVLVGLFGLFSEKIYIHV
                     LTIPILGIPLILPTMFTYMAIIMPSILSLRSQSTRPLNRPLRYLLGFVFPIIVSMPFI
                     FIHTLLTNAGLIDPTILTK"
     gene            7667..9517
                     /locus_tag="PL000_17740"
     CDS             7667..9517
                     /locus_tag="PL000_17740"
                     /inference="COORDINATES: protein motif:HMM:NF013438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HNC90777.1"
                     /translation="MKKLFMLGLVVLLSLSTAVPVFAETNASHAAVPNQFVDVPQNHW
                     AVAWINQLYTEGVTGGCSTAPLRYCPDAVVTRAQMAVFLVRVIHGSTFTPPATSVIFE
                     DVPADYWASAWINQLNADGVTGGCSSAPKLFCPEANVSRAQMAVFLLRAKYGSAYSPP
                     ASADVFSDVPADYWARDWIGQLYAEGYTGGCSSNPLQFCPEAFVSRDQMSVFILRAIH
                     GANYVPPPIDQTSLRPFPQHVQYAPGSILPDHRTQQQLDDDVRAFYDYWKANYLIAAG
                     VNADGIPMFRVAFGKNEPDRSATVSEGQGYGMLIVPIMAGYDPDAQAIFDGLWAFARA
                     HPSEGDPRLMDWKVPQTDGNDSAFDGDADIAFGLLLADAQWGSAGLVNYKAEAETMIA
                     GILESTIGPSSHLPMLGDWTDPNGVTYNQYTPRSSDFMLTNFRAYGQATGNPVWDDVV
                     AQSQGVVVAMQANHSPQTGLLPDFIVRKNGSYEPAPVNFLEADTDGAYSYNAGRDPWR
                     LGMDALLSDDAASRASALKISHWAESAVSGSPANFRAGYNLNGTPLPNSDYFTSFFVA
                     PLGVAAMNDSAQQDWLNAVYDSVYATREDYYSDSVTLLCLLVMTGNFWTP"
     gene            9514..10473
                     /locus_tag="PL000_17745"
     CDS             9514..10473
                     /locus_tag="PL000_17745"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HNC90778.1"
                     /translation="MNSIHSQSLVWDAHACFPLNPNSDLSELKRYKDSGVNFVSLNIG
                     MDMDSFENVIQVLACFRKYIAAHPDDLVLALAVDDVIGAKASGKLAVAFDLEGSDPLR
                     GDLNLISFYYDLGVRQMLLAYNKDNRASGGCMEGHIGLTDFGKQVIREMNRVGMVVDV
                     THMSYRATMEAFETSAAPVIFSHSNPKSLRDNARNITDEQIKACAKTGGVIAINGIGD
                     FLGGTTSEIMVKHIEYVMDLVGPEHVGIGLDYVVDKQELIDYIEGHPDVFPPDKMNDY
                     LAFVEPEQLPEITDLLHKNGHSEQVIAGILGGNFLRIAKQVWK"
     gene            10475..10975
                     /locus_tag="PL000_17750"
     CDS             10475..10975
                     /locus_tag="PL000_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC90779.1"
                     /translation="MQKKISTELTSANIASILALLQETPEKMETLSKAVSAEKLRQPL
                     GANERSFTETLAHLINSEARISESIYLALLVDEPLLADVHSEREWGKLLRFDLLDFPE
                     LLTYFKTRRKVLLRVLSALTEAQWARPIREEGKKRKESIYLRARSLALHELEHVNDLE
                     RKIGQG"
BASE COUNT         2862 a         2490 c         2799 g         2981 t
ORIGIN      
        1 atggggaggg atagttgttg tttgggggcg tataatttga cgtatatagg gtgtttgcgc
       61 ttgcaccttc cgcaataaaa accataagta aaaaaagata ttcaaaaggg aaaaatatga
      121 atcaaaaagt catcattttt ttcgctataa tccttttggc ggcttgttcg tcaagtcctt
      181 cccgtgtatc aactgaatct ccgtccgttg aaccttccca gacttccatt ccctctccat
      241 tacccttgcc gtcagataca cctgagccat ccccaaccgt cactcccatt ccgatcggtg
      301 gcgggcaatt gcttgttgca ttttatgcat ctggcagttg tggtgcatgt atcataatag
      361 gagatttttt tacgggagaa attttattcg agatcccttt atcaacagat tttcaaacgg
      421 ggaatatttt ctggtctccc gatggggaaa gcatccttta ttccgatatc actacagcca
      481 gaatgaatgt tcttttgttt gatttggaga cggggcaatc taaaaagctg ggtgatcacc
      541 cgcggaaggg tggatcgcca gaattcgtta attcaatgaa gtatgttgag tggtcatacg
      601 acagcgaata tgttatgtat gatgtgtatt atgaagacag taatcttcaa aagtcttatt
      661 ttgcctccaa aagcggggtg cctggttttt acgaaagcgg atttactgac tggtttcccg
      721 atagcaggac aatgttctca atttatggcg gaaaagattc gtacaatgtg gagacgaaca
      781 catttgtgcc aacatctaca gatgtgttaa gtaaattcaa cgttgtttat attttgaaag
      841 actttatcct gttggaaagg gagaagacaa agatctcagt cattccgttt cctgaaaatt
      901 ggaatgacgc tctggcctgg caatacgatg cacttggttc gcaagtcgtt gttttggctc
      961 agctttctga agaaattaaa aatggacagg tacatgttta tgttgccgat aaaattgatg
     1021 acaataaagt atctgttatt ggatctgtct tggtttcaaa tcagatgtcg tattttgtga
     1081 aaataatcga cttgaaaaac ttgcctgttg aaataaagcc agatgatttg ctgcagcaaa
     1141 taggggatac gccgcttgtc gtctcgcccg atggggatta ctacttgaaa ggttattgca
     1201 cttttatgga aacgtgtttg aattacaacc caaactggaa aaatgaaatt gtcagagggt
     1261 gggggtttca ggtcgttggg tttaatggcg ttgaacaggt gcttccagca gatttatccc
     1321 agttcaaagg ggtgacgaag acgactgcca atgtcattaa tacaagcgat gggctgcttg
     1381 atggaattgc cttttattgg aagtgatggc tttgaaaata aaaatgggcc agcaaggggc
     1441 agttgttgtt tggggatgta tatagggtgt tacacagtta gatgtataaa acttagttgg
     1501 atgggtgtgc catgaaaact cttggaggtg tgattatgaa agactattgg gcgatattga
     1561 attatgagat acaaatgtat ttcggtgcac gacaccttca aagttttcca atcaatggtg
     1621 ctagccaaag cctgacaacc ttacaggcaa gtgcaatgac agaagtaaaa gccctccatg
     1681 ttcggatttt gcttgacata ttccttgaaa gaaaaaatag tgacgacata aacattgatg
     1741 atatcctccc ggattggcga gagaagaatc aaaccgttgt tcacaatttg gaaattgctt
     1801 acaataaaaa acttgaaatc ggcaagacgc ctaagtggta tttgaacaaa tatcttgccc
     1861 acccagataa aaggagagga gatcactttg attgggctcc agtgattaaa cgaatggata
     1921 cgcctctaaa ggaagttttc aaaactttac caacagaaaa gttacacgca ctcgtatttt
     1981 ttcaaaaata tctttacgca taaggtgagt caaaaaacgc ctaacaaagc gtgcatctga
     2041 cgctggggac tgccgtaagg tgtgcggcaa tctcaagcat tttctacgcc ttgtcatttt
     2101 tttggttgga cggcttcgcc gtctctgcgt gggtaacgca atccgttggg cagttgattg
     2161 agttttcata attcacaggg aatcagatac atatggaaaa tgaatatgcg gaatggattt
     2221 actttggaat ttctttagta attgctgcat ggatcatagt agcaataaac tcctctgcca
     2281 aaaatacact catagggtat ctccgagaaa gaggggcaaa agatattgta gtcattcgtg
     2341 aatggcttag ttttgacaga acaataactt tttttgtaga gtttacgcac ccggatggca
     2401 acaagatttc aacatattgc aaatttggcc gcttaggaac atttgaagat ggagagatat
     2461 tttggtctgt gccaactaat ttagaaccgc cgaagcctaa aacaaagtaa taaaaattcc
     2521 tcccttcgta atatcatgaa tttagtgaga gaatttactc acagtttaac aaagtattgc
     2581 ttacttattg cagttataat agattccaga aacggggaaa atcttttcac tcaataacaa
     2641 gttttatggt taaaaactgc ccaacaaagc atgcgcttga ccggtgggat tctgcgcaca
     2701 tctcaagcag tcctctgtgc cttatcgttt ttccagttgg acggcttcgc cgtccctgcc
     2761 ccagcgtggg taatgcaatc cgttggcacg ccccgtgcaa agcgaaagtt gcaattaaaa
     2821 atcgaagttg aaacgagtaa aattagcggt ggaaaaaagg agtaaaaatg actctccaac
     2881 aaattactat cgaaattcca gaaaaggtac ttctcgcaga aaagacggat gctgaatctt
     2941 ttgggcgtga aattctcatt ttagcggcag tcaagttgtt tgaaattggc aggctttctt
     3001 ctgggcgagc atccgaactt gcgggaatgt ccagaattga gtttttgctc aacttgaacc
     3061 gctataaggt ttttccgttt gcctccgagc ttgatgattt agaaaacgcc catgcctaaa
     3121 gccatcagca acacttcccc actactttat ctatacaaga ttggcgcaat caatttactt
     3181 ccacaaatat tcgatgaagt ttggacagct gaatctgtca aaaatgaatt actggcaggg
     3241 cggagcaaag gctatgatgt tccaaatcca gcagattatc cctggttgaa catcgtcaat
     3301 ccaaaatcca tgccttctga atggcttgcg ttagatttgg gagcgggtga acttggcgca
     3361 atggctttgg ctttagaaaa tcccgatcgt attgttttgc ttgatgacat gcttgctcgt
     3421 cgtactgcac aggtagcggg tttacaggtt tggggaactt tgaaagtttt actggaagca
     3481 aaatctcaag gtttgattga aaagattgaa ccgtatatat cgaaattaag tgacgcaggt
     3541 atgtgggttt ccgctgaagt aaaacagcgt attcttattt tggcgggtga gtcttaaagt
     3601 caaaagcgtg ccaacaaagc gtacctctga cactggggac tgccgtaggg cgggcgcaaa
     3661 tcttaagcag gtttctgctt ctgagcagag tccacgagcg cacaagatat ggacagtaaa
     3721 agatgaaaaa gacacctgtt gcaaccagga aaccaaagag ttcgtccact gttgtcaaag
     3781 cggaattgag gcgggatgcg cttatcgcag acttgatcga gactcgcaga aaaatcgttg
     3841 atgtggcttc gtctttgtca ccgaccgagc aagatcaagt ctttttgggc atttggtcca
     3901 tgaaagattt gttggcccac ttgataggat ggcttgaaga aaatcgcaag gcggtgaaaa
     3961 cgatccgcgc gggcaagctc cccgaattct atgcgtataa agatcgcgat tggcaaactt
     4021 ataatgcgta tctcgtctcg aaatataaac gtggagacat gacagagtta ttggcgaagt
     4081 tgcaggtttc ccagcagaaa ttcataaaat caattcgggc cgttcctatt gaggtacttg
     4141 aacaggattt tggggtaagg tttaatcggt ataaagtcac aattgtccgc ttgttgcagg
     4201 ccgaactcaa ggacgaaaag gtacattacg ctcaaatgga agcgtttagg aataaggcga
     4261 agtaaaatta acagcggacg caatcgaaac ttgaagggta tgattgctgt tctcaatcgc
     4321 agagagagta cattcccaag aggcgtttat gcaaaacgaa ggtgaaagca ggtaatggag
     4381 atacatattc gtaaagcaac tgcaaatgat tatgacgatt tatgtgaact cttcgatgaa
     4441 gtggatgctt tgcatcgcgt taatctgccc catgtttttc aaaagtcgac tggccctgct
     4501 cgagaaaaag attattacct ggggctgatt gccgatgaga acattgggct ttttgttgcg
     4561 gaaatggaag agaaacttgc agggtttgtg catgtgcttg tccgagatac ccctgtcttt
     4621 tccatcgtga tcccccgctg ttatgcaatc gtcgacggga tcgccgtaaa atctgaattt
     4681 cagaagcatg gaattgggaa aatgctgatg gacaaagcgc aggaatgggc agagaccaaa
     4741 gacgcggaat tcatggaact gaatgtctac gagtttaaca ggaacgcgat ctccttttac
     4801 gaaggtctcg gctatcaaag ttccagcagg aaaatgagaa aggaaatcag caaggcaggg
     4861 tgaaaaactg tagcccggac tggttaagtg caacactgaa attcgaattg aattgaaaaa
     4921 acaagatcag gttttgaaaa ttatcggtcg tccttcttaa tcttactttg gagagaataa
     4981 aatgcctgaa aacgtatcaa cctaccagat cgtggatctg atgcctgact tctgggaatt
     5041 ccatgcaaaa aacagggatg cagatgtttc agaacaggca tcccaattta aaagaatggt
     5101 tgtggataaa caccccgaag cctacgccgc cagggtcatt ggccttgatc caaacaaacc
     5161 ctatgacgaa gcacttgccg agcgttacct gctttgccat gagcgcagca cggctcaaca
     5221 agtcagcacc atgcagagga tatccgcttc gatcgcacgt gacatgtccg cgtatgaaga
     5281 aaagtttcgc aaggtttttc ccgacttcaa ctatacaggt tcggtttact tcatgaattc
     5341 gctcctcggt tttgatgggg ccacgcgtac ggtcaatgga aagatcgccc tgttatttgg
     5401 tttggatacg atggcatggg tgtacggcga tgatctggac ctgcagccat ttttccatca
     5461 tgagattttt cacatctacc attctcaatt tctggaagat gatagcgatg aagtcgccgc
     5521 cgccgtctgg caggaggggc tcgctcaatt tgtggcgaag tcgttgaatc cagatgccga
     5581 gggcgtgaat ttgtttgggc ttccaaagga aatgccgcag cgctctgaag caatgctctc
     5641 cacacttgcc agtggtcttc ttggtgtgct tgattcaacc agcagagatg attacggcag
     5701 atactttttt ggaaacaatg gcagtgaaga gattccaccg cgtagtggtt actatctgag
     5761 ttatctggtt gtgcagaaac tttcgaaaaa atactccctg caggaaatgg ctcattcgaa
     5821 actggcagcc atcaaacccg aaatcaaaac ggctttggaa gaattggcca acggcggaag
     5881 atgaccatgc aaggagaaac aatggaagcc tacgatgacg atctcggcag atttgaagcg
     5941 gaaggagccg caccactgcc agattctgga aatcagggat atatcgatca tgacaacgcc
     6001 cgcatctggt acgcagacta tggggtgggt ccaaccgtgg tgttgttgca cggcggcctt
     6061 ggccatagcg ggaattgggg atatcaaatc cccaccctga tcgggaatgg ctaccgcacc
     6121 attgtcatag acagccgcgg acatggccgc agtacgcgtg atgaccgtcc atatacctat
     6181 gagttgatgg cctccgatgt tttggccgtg atggatgttt taggagttga aaaagcggcg
     6241 ctggctggtt ggagtgatgg cgcatgtatc gcgttggtcc ttgctgcaca ggtgcctgca
     6301 cgggtggaag gtgtcttctt ctttgcttgc aatatggatc cgagcggcgc gaaggaaatt
     6361 accgagttcg ccccgattct ccagcggtgt atcggcaggc atatcaaaga ctatggtcaa
     6421 ctgtcagaaa cgccagacca gtttgaggcg ttgtctgaag cggtgggtct catgcagagg
     6481 acacagccca attactccgc caatgacctc gcgcagatca ctgtgccggt tgcggtcatc
     6541 cacagtgagt ttgacgaatt catcaaactt gaacatgctg aatatcttgc acgcagcatt
     6601 ccaaacgcgg actttgttta tctttcgggt gtgagccact ttgccccatt gcagcggcct
     6661 gaccaattca acgacacgct tctcaatttc ctcggcaaag tgctggtgtg aggcgcctga
     6721 aaagttttag accgatcttg ttttaacaac atggtcgtca tggtcgtttg ggggtgcatc
     6781 tcaaaccttg aaaacgcata gtaaaatcca cgcacaattg ttttgcataa aaccgtcgtg
     6841 cgtggatttt ggttttcacg gcggaatttt ttgaaggatc gttatgactg ataaacccag
     6901 cacaacccag ccgatcatgg atagacgcct tcttgtcttg cttgttctat ccgttgcgtt
     6961 tcttgtcatt gcctttcttg gcggccagcc ttcatcagca cgcggtttct attatgccgt
     7021 ttggctgaca ttctcaggtt ggctatggaa gaagcgagag cgcctgagcc tctggctgca
     7081 agcccgtcca ttgcctgaat ttccgctttt tgttgggctg ggtctcgtca tgatccttgt
     7141 tgaagaaacc attgctgggg tggctgtcca cttgctgaat gtggacagtg tagggcagtt
     7201 gtttgccgtt gtgcctttgt attatttcaa caacctgctt ttgctgcctg gcttcatcgt
     7261 cggctggtat gttctgctca agttttgtgc atactcgcgg actgaggtgt tcgtcctcgt
     7321 gggactcttt gggttgtttt cggaaaagat ctatatccat gtcctgacga tccccattct
     7381 tgggattccc ctgatccttc ctacaatgtt cacctacatg gcgatcatca tgccgtcgat
     7441 cctttcattg agatcgcaaa gcacccgtcc gttgaacagg ccgcttcgat acctgctggg
     7501 gttcgtgttt ccgatcatcg tttcaatgcc ttttattttc atccacactt tattgacgaa
     7561 tgcgggttta attgacccga ccattttgac aaaataaacc tgttctcgtt gtgacagccc
     7621 accgtcattc cccgtctgag atgatttatc gttggaggag cctgtgatga aaaaactgtt
     7681 catgcttggg ttggttgttc tgctttcgct ttcaacggct gttcccgttt ttgctgagac
     7741 caatgcatct cacgctgctg tgccgaatca gtttgtggat gttccgcaga accattgggc
     7801 ggtggcgtgg atcaaccagc tttacaccga aggtgtaacg ggcggatgca gcactgctcc
     7861 cttgcgatac tgccccgatg cggtggtcac tcgcgcgcaa atggcggtct ttcttgtgcg
     7921 tgtcattcat ggcagcacat tcacaccgcc agccacttcg gtgatctttg aagatgtccc
     7981 agccgattac tgggccagcg catggatcaa tcagttgaac gccgatggcg tcacaggcgg
     8041 atgcagcagc gccccgaaac tattttgtcc agaagcaaat gtcagccgcg cacagatggc
     8101 ggttttcctc ctccgcgcca aatatggcag tgcctatagt ccacctgcct cggcggatgt
     8161 cttcagcgat gtgcccgcgg attattgggc ccgtgattgg atcggacaac tctacgccga
     8221 aggctacacc gggggctgtt catccaaccc tttgcagttc tgccccgaag catttgtcag
     8281 ccgcgatcag atgtctgttt ttatcctgcg tgccattcac ggcgctaatt atgttccccc
     8341 gcccattgac cagacctccc tgcgcccgtt tccgcagcat gtccagtatg cgcccggcag
     8401 cattcttccc gatcatcgca cccagcagca gcttgatgac gatgtccgcg ctttttatga
     8461 ttactggaag gcgaattacc tgatcgcagc gggcgtgaat gccgatggca ttccgatgtt
     8521 tcgcgtggcg ttcggaaaaa atgaacctga ccgttcagcc accgtatctg aagggcaggg
     8581 ctatggcatg ttgattgtcc ccatcatggc ggggtatgac cctgatgcgc aagccatctt
     8641 tgatggattg tgggccttcg cgcgtgcgca tcccagtgaa ggcgatccgc gcctgatgga
     8701 ttggaaggtg cctcaaaccg atggcaacga ctcagccttt gacggcgatg cagatattgc
     8761 cttcggttta ctgctggcag atgcccaatg gggcagcgca gggctcgtaa attacaaagc
     8821 cgaagcggaa acaatgatcg caggtatttt agaatcgacc atcgggcctt ccagtcatct
     8881 gcccatgctc ggcgactgga cagatccaaa tggtgtgacc tacaaccaat acacgccgcg
     8941 ttcctccgat ttcatgctga ccaattttcg cgcgtatggg caggctacgg ggaatccagt
     9001 ttgggatgat gtggttgcac aaagccaggg tgtggttgtt gcaatgcagg ccaatcacag
     9061 tccgcaaaca ggtctgctgc ctgattttat tgtcaggaaa aatggcagtt atgaacccgc
     9121 tcccgtcaat tttctggaag cagataccga cggcgcctac agctacaatg ctgggcgcga
     9181 cccgtggcgt ttgggtatgg atgccttgtt gagcgatgat gccgcctcac gtgcaagtgc
     9241 gttgaagatc tctcattggg ctgaatctgc ggtgtctggc agccctgcaa acttccgcgc
     9301 tggatataac ttgaacggta cgcctttgcc aaacagcgat tacttcacgt cgtttttcgt
     9361 ggccccgttg ggcgttgcgg ccatgaacga ttccgcccaa caggattggc tgaacgccgt
     9421 ttatgattca gtctatgcca ctcgtgaaga ttattattca gacagcgtca ctctgctctg
     9481 cctgctggtg atgacaggaa acttttggac accatgaact caatccactc tcaatccctc
     9541 gtctgggatg cacatgcctg cttcccgctc aaccccaact ccgatctgag cgaattgaaa
     9601 cgctacaaag atagcggcgt gaatttcgtc tccctgaata tcggcatgga tatggattcc
     9661 ttcgagaatg tgattcaggt gctggcctgt ttcaggaaat atattgccgc gcatcctgac
     9721 gacctcgtgc ttgctcttgc ggtggacgat gtgatcggcg cgaaagcatc tggcaaactc
     9781 gcagtggcct tcgacctcga aggctccgac cccttgcgcg gagatttgaa tctcatttcg
     9841 ttctactatg accttggcgt ccgtcaaatg cttctggctt ataacaaaga caaccgcgcc
     9901 tcaggcggct gcatggaagg tcacatcggc ctgaccgatt ttggaaaaca ggtgattcgt
     9961 gagatgaacc gtgtgggcat ggttgtggat gtgactcaca tgagctatcg tgccacgatg
    10021 gaggcatttg aaacctcggc ggcgcctgtc atcttttccc attccaaccc caagtcgctg
    10081 cgcgataatg cgcggaatat caccgatgaa cagatcaaag cctgtgccaa aacaggcggc
    10141 gtgattgcga tcaatggcat tggtgatttt ctcggcggaa ccaccagcga gatcatggtc
    10201 aagcacatcg aatatgtaat ggatctggtc ggccccgagc atgttggcat tggtctcgat
    10261 tatgttgtgg ataagcagga attgatcgat tacatcgagg gtcatcctga cgtatttccg
    10321 cctgacaaga tgaacgatta tctggccttt gttgagccag aacaactccc tgagatcaca
    10381 gacctgttgc ataaaaatgg tcacagtgag caggtgatcg ctggtatttt gggtgggaat
    10441 tttctgcgga tcgcaaaaca agtctggaaa tgacatgcaa aagaagatct ccaccgagtt
    10501 gacctcagcc aatattgcca gcattctggc tttgcttcaa gagactcccg aaaagatgga
    10561 aactctgagc aaagcagtct ctgctgaaaa actccgtcag ccgctgggtg ccaacgaacg
    10621 ttccttcacc gaaacactgg cgcatttgat caacagcgaa gcacggatat cagagtccat
    10681 ctacctcgct ttgctcgtgg atgaacccct cctggcggat gtccactctg aacgggagtg
    10741 ggggaagttg ttgcgatttg acttgctgga tttccccgag ttgctgacgt atttcaaaac
    10801 ccgccgcaag gttttgctga gagtcctgtc ggcactgacc gaagctcaat gggcaagacc
    10861 aattcgcgag gaaggcaaga agcgaaaaga atccatctac ttgcgggcga ggtccctggc
    10921 gctccatgag ttagagcatg tcaatgatct ggaaagaaag atcggtcagg gttgagattc
    10981 tgctacgatg ctttttttga atccgcgtaa attcgcgcaa ttcgcggcaa agtttttcaa
    11041 atggatttcc tcaatgggga cgcgcgactg gaaaacaaaa aacacaccca aaacagagtg
    11101 tgttttattt gtggcgggga gggcgggatt cg
//
LOCUS       DAOQLC010000132        10902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2334_length_10902_cov_24.324329, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000132 DAOQLC010000000
VERSION     DAOQLC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2334_length_10902_cov_24.324329"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PL000_17755"
     CDS             complement(<1..357)
                     /locus_tag="PL000_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HNC90780.1"
                     /translation="MTPKVETSTPPHTPTPIGPYNHIAKVGTFITIGGTAGVDPATGK
                     LAGDDVFSQTVQILKSFQVMLESVGSDLDHVVHINIFLKNMSDFEEMNRAYISKMGNH
                     RPARTVIGVNELPKPGV"
     gene            501..2165
                     /locus_tag="PL000_17760"
     CDS             501..2165
                     /locus_tag="PL000_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNC90781.1"
                     /translation="MKKQTVLPFLVAVLVLSLACQTLTNPGAQASPTPPILFSTPETP
                     APQTGSAQLAGLTYATHDSGTDSNQLWQIGSDGKAVVISESGYAIFSPDGTQAAYIDF
                     NGQCSWLLDYEKKEARPLDCTGSNNPEFFELDEVIGWAAGQPDTLFMLFNESFGIGGG
                     TGYFGTLSTSTGEKRIFDNQHAVSTVLPSPDGRTVLFSNFDFAALYTLGSDSQPIDLG
                     QYKLGEFKLAYPAWSPDGKTIAWGLMNENGDLKYALGIMDPQTKTSKILHPYSPMLAP
                     VFADGPYVSTPQMSSPKPIWSPDGQWLAFEAYVYSQDQNNPQREAWVIRADGSAENKL
                     DGKFINWSPDGQWVLYFKPLPNTPSKADLMAAHPDGSNAARIAEVTFDEFLTQAVWSP
                     DSKHLLLRDGETVALTEAGSWQLNSLSGTEIPPDARLLAWLDSFPMPFSQLTVLPLPT
                     PSAASDFSCPTAPPTRLSVGATGRVTYTDGQTIRLRSAPEAGDNVIDQLPEGTEFEVI
                     DGPVCTPRIDRADAFVYWKVKVPTRNGISGWLAEGTFNAYFIEPWP"
     gene            2243..3829
                     /locus_tag="PL000_17765"
     CDS             2243..3829
                     /locus_tag="PL000_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90782.1"
                     /translation="MKYFKKQDLIWMPPLALGLGAVLSLIQPGNWFFGWLESSLLFLL
                     CFLLLTVSARWANGASTVPDTSKTLAWMLAVAFALRFFGGVATYLALPVDGFADEDDK
                     AGYVYTDAHRRDGQAWELAGSEHPILDAFNKKFAYDQYGGLLAISAFVYRYISPDAHR
                     PLLLVLLASFAGTLCLSFLWKAVTLQWGQKVAVAAGWVFALYPEGVLLGGSSMREPYL
                     MLFSAFTLWGFVIWQKTQDKKTLIWFGLGILGMLLVSPVVALVTLALFAGWVYFTREH
                     SQLSWWGALAISVVFIAGLFWLASTLDRQGNLGGGTPLAVINNFLREAIKMDLHHLER
                     GSGWVQKLFDEMPKWLRLPFVLVYGVFQPVLPAAFVEPTTFTWRVIAIARAVGWYALL
                     PALILSFGAAAGQQSDEKRKLLVWLSLVAWFWVLLTSLRGGADQWDNPRYRTIMFLWQ
                     AILAGHVWVWWRESRNAWFLRVVAMEVVFLLVFGQWYASRYFHLGGQMQFAQMVAVIL
                     GGWVLILLGGWWQDRKKGNP"
     gene            3869..4831
                     /gene="gmd"
                     /locus_tag="PL000_17770"
     CDS             3869..4831
                     /gene="gmd"
                     /locus_tag="PL000_17770"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNC90783.1"
                     /translation="MPTALITGITGQDGSYLAEFLLKKGYRVVGVARRSSTVTYERIQ
                     HILDDITVVQGDLHDQGSLLSFLEEYRPTEVYNLAAQSFVPTSWNQPALTGDVTALGV
                     TRLLEAIRFVDPKIRFYQASSSEMFGKVMEVPQRESTPFYPRSPYGVAKMYGHWITVN
                     YRESFNMFATSGILFNHESPRRGLEFVTRKISDAVARIKLGMSKELRLGNLEAQRDWG
                     FAGDYVEAMWLMLQQNQPDSYVIGTGETHSVREFCEIAFSHLNLDYKQYVVQDEKFYR
                     PAEVDLLISDPSRARTELKWEPAVSFKELVTMMVDSDLARLKKG"
     gene            5030..6850
                     /locus_tag="PL000_17775"
     CDS             5030..6850
                     /locus_tag="PL000_17775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC90784.1"
                     /translation="MLAILGVILYVAQAWRYAHTTIPGLDEGSYLFKGYLYLHGVYEP
                     FEPYGPLTNKAPFAFLIPGFAEYIFGAGLRTGRFFMIFLGLLAILGTWITARRWAGNW
                     FATATVWAYALAPTVIKIHAITASEVIIACMLAWMCVFLLAEERPLWQIVAGSVLGAL
                     AVLTRQNMVVVLPLLILYVFWQHGKQKGLVSFGTASAIFLAVHIYYWPNILTIWAPWL
                     PDGLTPFLNPFRLPKEAVPVWDPSIDFWNRTISFFQGIRYHFIPAAGSIFALMILRIP
                     RDWKTVPAMRAALFLALTYFILFAMHGWAAVASQYESYSCVYCFTPYLSFFDPLGILF
                     TVIVLSHLANRTPSRWILFLGAATLLTLATGFGFSSFDSLSQFFLKFPVPRMSGGQFL
                     PGTTTLLAFITNKFGTELIWVKRYVSAALGLLVGVAVLLIAGIITRRMKDAQTRPGFA
                     FVLLNLSIAMGLILSPLLHVGGRSIDCETDMLVANEQVGAHLASFIPADSLVYWDGGL
                     SFTAMTYVPQARIFPPQINDGYSYRIGGDADTLARFSHWNTELKEEWKASADIFIIQS
                     KRYTLWKDFLNPNEFEEYPKPPVAPSCEDGSELRIFHRLP"
     gene            6847..7809
                     /locus_tag="PL000_17780"
     CDS             6847..7809
                     /locus_tag="PL000_17780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC90785.1"
                     /translation="MNLSLVVPVYNEQDNLPLLFEAINKVMPTMNRTWEVILVDDGSQ
                     DNSLAVLNEYAKKDFAHVRVISFRRNFGQTAAIAAGLDYAQGEIIVLLDADMQNDPAD
                     IPMMLAKLDEGYDLVSGWRKSRKDNAITRNFPSMIANRIISKVTGVHLHDYGCTLKAY
                     RRDVLEGFRLYGEMHRFIPVYAHSVGAKITEVVVNHHPRKHGKTKYGLERTAKVVLDL
                     FTVKFLISYSSKPIYLFGGAGGFLMVISAIIMLYLFVRRIFFLVAIANSPLLQMSAMF
                     FILGFQSILLGLIAELLVRTYHESQRKPTYTIRTMINLEQDPRD"
     gene            7802..8707
                     /locus_tag="PL000_17785"
     CDS             7802..8707
                     /locus_tag="PL000_17785"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC90786.1"
                     /translation="MINWLSKNRQTVARGIGSVLALVLLAVLIQEQGDGELFSAFRRV
                     SLGYFGLALVVLLVSRLFAILRWHILLRSAGVNISFLRSVELTFTGNFSSNFLPTTIG
                     GDVVRLTGAMQLGYDRAICLASLVVDRLVGMAGMALALPFGLVPLFALSNGSTQSVTL
                     SALLQKGVDFIRRTFESLSIWLKKPSALFASLLATLGNQAFIYLMVYFLLLGIGHRLS
                     FWLVAGMYTLSYFVTLIPISINGLGVQELSMTFLLTSLGGLTESESATVAVLVRIIFL
                     ITSLPGAYFLPSILAAMNSKEVDER"
     gene            8771..10732
                     /locus_tag="PL000_17790"
     CDS             8771..10732
                     /locus_tag="PL000_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90787.1"
                     /translation="MKPTSSKIFWQTFITATVLMSGLAAYQTVQRVADLEITLWRSKW
                     ILLVGLFALNIFAGMFVLWQLSTARASYLTEKQAAVTKSGINPWAGSVLIAFGFFLVW
                     AVRLFFFGDVLPQIAPIFWVFLWASLIQTFGLQLLTGRDWLTPFVAAVLVQGVVYQIF
                     GHLTIVSAYPFSIGYSEAGRHYYASLFFARPLYGTELALPFLHPTRYFLMSLPFLVDG
                     LPLWAHRLWQALLWIGLTAASSILLIRRLNFTGWVRFALIAWAFLYFFQGAVYYHLQV
                     CVILILAGVSVKHPWRSLLFVVLASVWAGASRVNWFPVPAMLAVAIYLLETPLEGKGW
                     RYWLTPFLWGICGLAAASASQFAYIAVSGNSDARSFGSSFTSDLIWSRLLPNETFPMG
                     ILLGIVIVSAPLIFAFIQMVRGKISNLHPLRWLALIAMLAVLFLGGMVVSVKIGGGAD
                     LHNMDAFLVMLALVVTAFIGGRVAGEREANPAWGAIPVWVVAAAALVPIGFALPQVGF
                     FQRYDSNLVEADLFKLQASTQNIEGEILFVTERQLLIFNDVNNIPLVPEYEQMELMEM
                     AMSGNRDYLENFYADLRSHRFALIVAEEQKFALQKHGAFLEENNAWVRFVGAPILCAY
                     KPVTTLSSTNIQVFAPRENNPECKDPFEK"
BASE COUNT         2099 a         2914 c         2956 g         2933 t
ORIGIN      
        1 cacaccgggc ttgggtaatt catttacacc gatgaccgtc cgtgcggggc ggtggtttcc
       61 cattttgctg atgtaggcgc ggttcatttc ttcgaagtcg ctcatgttct tgagaaagat
      121 attgatgtga accacgtggt ccagatccga gccgacggat tcgagcatca cctgaaagga
      181 ttttaggatc tgcacggttt gcgaaaagac atcatcaccg gcgagcttgc ctgtggcagg
      241 gtccacgcct gcggtgccgc cgatggtgat gaaggtccca actttggcga tgtggttgta
      301 tggcccgatg ggggtggggg tatggggcgg tgtggaagtt tcaactttgg gggtcatggt
      361 cactccttgt gttttgattg cattgtaccc tgctgttttt gaaaaccatc tttgcagtta
      421 aacctctgca gctatttttt agctaacatc gcaaaaccga taaaaaaggg gaatataatt
      481 gcatcgctca aggaggagcc atgaagaaac agacagttct gccctttctc gttgccgttc
      541 ttgtcctctc gcttgcctgc cagaccttga ccaaccccgg cgcacaagcc tctcccacac
      601 cgcccatttt attttccacc cctgaaacac ccgccccaca aacaggctct gcgcaattgg
      661 ccgggctcac ctacgccacc cacgactcgg gcaccgattc caatcaactc tggcagatcg
      721 ggagcgatgg caaagccgtg gtcatttctg aaagcggata cgccatcttt tcaccggatg
      781 gcacgcaagc cgcctatatc gatttcaacg gtcaatgctc gtggcttctc gattacgaaa
      841 agaaggaagc cagaccgctc gattgcacgg gttccaacaa ccccgaattt tttgaacttg
      901 atgaggtcat cggctgggct gcaggccaac ccgataccct gttcatgctt ttcaacgaat
      961 cctttggcat aggcggtggc acaggttatt ttggtaccct ctccacctcc accggcgaaa
     1021 aaaggatttt cgataaccaa cacgcagttt ccaccgtttt gccctcaccc gatggcagga
     1081 ccgtgctttt ctcaaacttc gattttgccg cgctttacac tttgggcagc gattcccagc
     1141 ccattgacct cgggcaatat aagctgggtg aattcaaact cgcctacccc gcatggtcac
     1201 cggatggcaa aacgatcgca tggggcctga tgaatgaaaa cggcgacctc aaatatgccc
     1261 ttggcatcat ggacccgcaa accaaaacct caaagatttt gcatccatac tcccccatgc
     1321 ttgcccccgt ctttgccgat gggccatatg tttccacacc acaaatgtct tccccgaagc
     1381 caatctggag tcccgatggt caatggctgg ctttcgaagc ctatgtctac agtcaagacc
     1441 aaaacaaccc ccaaagggaa gcgtgggtca tccgtgccga tggcagtgca gaaaacaaac
     1501 ttgatggaaa attcatcaat tggagtccgg atggtcaatg ggttttgtat ttcaaaccac
     1561 tgcccaacac cccctccaaa gccgacctga tggccgccca ccccgatggc tccaacgcgg
     1621 cccgcattgc cgaagtcacc tttgatgaat ttttgaccca agccgtttgg tcaccggatt
     1681 caaaacacct gctgttgcgg gatggagaaa cggtcgcact caccgaggca ggctcctggc
     1741 aattgaacag cctctcaggg acagagatcc ccccagacgc aagactcctt gcatggctgg
     1801 attccttccc catgcctttc tcccagttga ccgtccttcc gttacccact ccctctgccg
     1861 catctgattt ctcctgcccc accgcgcctc ccacacggtt gagcgtggga gccacaggcc
     1921 gagtcaccta caccgatggt cagacgatcc gcctcaggtc cgcaccggaa gcgggagata
     1981 atgtgataga tcaactgccc gaaggcaccg aattcgaagt cattgatggc cccgtctgca
     2041 ctccgcgcat tgaccgtgcc gatgcctttg tctactggaa ggtcaaggtc ccaacgcgca
     2101 acggcatttc aggctggctt gccgagggta ccttcaacgc ctacttcatc gaaccgtggc
     2161 cgtaaagaga aaacacaagc aggtttgttt gtctaagaat gtgcggcttt tagactcgcc
     2221 tacttcgtgg taaaaaaggg gaatgaaata tttcaaaaaa caggatctga tttggatgcc
     2281 gcctctggcg cttgggctgg gggcggttct ttcgctcatt caacccggta actggttttt
     2341 cggctggctg gaatcttccc tcctctttct gctttgtttt ctgctgctga cagtttcagc
     2401 acgttgggca aacggcgctt cgactgtccc tgacacgagc aagacccttg catggatgct
     2461 ggccgttgcc tttgctttgc gtttctttgg gggtgtggca acctaccttg ccctgccagt
     2521 ggatggcttt gcagatgaag acgataaagc gggctacgtc tacaccgatg cgcatcgccg
     2581 cgatggacag gcctgggagt tggcagggtc cgaacacccg attctggatg cgttcaacaa
     2641 gaaatttgcc tacgatcaat acggcggcct gctggctatc agcgcctttg tatatcgcta
     2701 catttcgcct gatgcccacc gcccattgct gctggtgttg ctcgcatcct ttgcagggac
     2761 gttgtgcctg tcctttctat ggaaggctgt aaccctgcaa tgggggcaga aggtggcagt
     2821 tgccgcgggg tgggtcttcg cgctgtaccc ggaaggtgtt ttactcggcg ggtcttcgat
     2881 gcgagagccc tacttgatgc tgttcagtgc atttactttg tggggattcg tcatttggca
     2941 aaagacgcag gataaaaaaa ctttaatttg gtttgggctt ggcattcttg gaatgctgtt
     3001 ggtttctccc gtggtggcgt tggtcacttt ggctcttttt gcgggctggg tttatttcac
     3061 tcgagagcat agccagcttt cgtggtgggg ggcgctggcg atctcggttg tgttcattgc
     3121 cgggttgttc tggctggctt ccactttgga caggcaggga aacctcggcg gcggaactcc
     3181 gctggcggtg atcaacaatt ttttacgtga agccatcaaa atggacttgc atcatttgga
     3241 gcgcggctcg ggctgggtgc aaaagttgtt tgatgaaatg ccaaagtggc tgcgcctgcc
     3301 ttttgtgttg gtgtatgggg tctttcaacc tgttttaccc gccgcctttg tggagccaac
     3361 cacgtttacc tggcgggtca ttgcaattgc acgcgccgtg ggctggtatg ctttgctgcc
     3421 ggcgctgatc ctctcgttcg gagcagcagc aggccaacag tcagatgaga agcgtaagct
     3481 cctggtctgg ctgagtctgg tggcgtggtt ctgggttctg ctcacctccc tgcgcggcgg
     3541 cgcggaccag tgggacaatc cgcgttatcg cacgatcatg ttcttgtggc aggccattct
     3601 ggcggggcat gtctgggttt ggtggcgtga gtcgcgcaat gcgtggttcc tgcgtgtggt
     3661 tgcaatggaa gtcgtttttc tgctggtctt cggacagtgg tatgcaagcc gttatttcca
     3721 ccttggcggg cagatgcagt ttgcccaaat ggtggcggtc attcttggcg ggtgggtgct
     3781 gattttgctg ggcggctggt ggcaggaccg aaaaaagggg aatccgtagt gtataattca
     3841 gcgtctgaat tttgaataag gagttaacat gcccactgca ttgatcactg gcattacagg
     3901 tcaggatggt tcctaccttg ctgagttttt gttgaagaag ggatatcgtg ttgttggcgt
     3961 tgcacgccgt tcgagcacgg ttacctatga acgcatccag catattttgg atgatattac
     4021 cgttgtacag ggcgatttgc atgaccaggg gtctttgctt tctttccttg aagaataccg
     4081 cccgaccgaa gtatataacc ttgcggcgca gtcttttgtg ccgacctcat ggaatcaacc
     4141 cgcgctgaca ggtgatgtga ccgctctggg tgtgacccgc ctgctggaag ccattcgttt
     4201 cgtagacccg aagatccgtt tttatcaggc ttcatccagc gagatgtttg gcaaggtgat
     4261 ggaagttccc cagagagaat ccacaccgtt ctatccgcgc agcccgtatg gggtggcgaa
     4321 aatgtacgga cattggatca cggtcaatta ccgcgagtcg ttcaatatgt ttgccacgtc
     4381 gggcattctg ttcaaccatg aaagtccgcg ccgcgggttg gagtttgtga cccgcaagat
     4441 ttcagatgcc gtggcccgca ttaagctggg catgtcgaaa gagctgcgtc tgggtaacct
     4501 tgaagcccag cgcgattggg gctttgcagg cgattacgtg gaggccatgt ggctgatgct
     4561 gcaacagaac cagcccgata gctatgttat cggtacaggc gagacccatt cggtgcgtga
     4621 gttttgcgaa attgccttct ctcacttaaa ccttgattac aaacagtacg tggtgcagga
     4681 tgaaaaattc taccgccccg ctgaagtgga tttgttgatt tccgatcctt ccagggcgcg
     4741 aacagagttg aagtgggagc ctgccgtttc attcaaggaa ctggtcacca tgatggtgga
     4801 ttcggatctg gcacggctga aaaaggggta gcccttttcc attggttcta tgaatagtac
     4861 tctgttcggt ctcttgcgta gaatgcaagg tcgcaatact gaatcccgtt ctgaaccaca
     4921 gaggcacgaa gacattgggg aaagttcaaa aaaactccgc gcttctgtgt ctccgtggtt
     4981 aagtttttcg actttcaaca gaaaagattg gcaatcctgg gtgccgggtt tgcttgcaat
     5041 tcttggggtg attctgtatg tggcacaggc gtggcgttac gcacatacca ccatccccgg
     5101 tctggatgaa ggctcctatc ttttcaaagg atatctttat ctgcacggcg tgtatgaacc
     5161 tttcgagcct tatggtccgc tgaccaataa ggctcctttt gcatttttga tccctggttt
     5221 tgcagaatac atttttgggg caggtctgcg cacggggcgc tttttcatga tctttctcgg
     5281 cctgcttgcc attttgggga cgtggatcac cgcacgccgt tgggcaggga attggtttgc
     5341 aacggccacg gtctgggcgt atgcactcgc gccgacggtg atcaagatcc atgccattac
     5401 cgcttccgag gtcatcatcg cctgcatgct ggcgtggatg tgcgtcttcc tgcttgctga
     5461 ggagaggccg ctgtggcaga tcgtggcagg gtcggtgctt ggtgcgctgg ccgtgctgac
     5521 gcggcagaac atggtcgtgg ttctgccttt gctcatcctg tatgtctttt ggcagcatgg
     5581 caaacaaaaa gggctcgttt cttttggtac ggcttccgcc atctttttgg cggttcacat
     5641 ttattactgg cccaacatcc tcaccatttg ggcgccgtgg ctgcccgatg gactcacccc
     5701 tttcctgaat ccgttccgcc tgccgaagga agcggttcct gtttgggatc cgtccattga
     5761 tttttggaat cggacgattt cgttttttca gggaatccgc taccatttca ttcccgccgc
     5821 tgggagcatt tttgcgctga tgatcttgcg catcccacgg gactggaaaa ctgtccctgc
     5881 catgcgagcg gccttgttcc tcgccctgac atatttcatc ctcttcgcca tgcacggttg
     5941 ggcggcggtg gcgagtcagt atgaaagcta tagctgtgtg tactgcttca cgccgtatct
     6001 gtcgtttttc gacccgctgg gaattctttt tacggtgatc gtcctctcgc atttggcgaa
     6061 tcgaactcca tcccgttgga tcctgttcct cggcgcagcg accctgctca cccttgcgac
     6121 aggattcgga ttttcatcct tcgacagcct cagccagttt ttccttaagt tcccggtccc
     6181 gcgcatgagc ggcggacaat tcctgccggg tacaaccacc ctgctggcat ttatcaccaa
     6241 taagttcggc acggaattga tctgggtcaa gcgttatgtt tctgcggccc tgggtttgtt
     6301 agtgggtgtt gcggttctgc tgattgcagg catcatcacg cgccgcatga aggatgcaca
     6361 gactcgcccg gggtttgcct tcgttttgct caatttgtcc atcgcgatgg ggctgatcct
     6421 ttctccgctg ctgcatgtgg gtggccgcag tattgactgc gaaacggata tgctggtcgc
     6481 caacgaacag gtcggcgctc atctcgcaag tttcatccct gcggatagtc tggtatattg
     6541 ggacggcggt ctgtcgttta ctgcgatgac ctacgtgccg caggcgcgta tctttccgcc
     6601 gcagatcaac gacggttatt cgtaccgcat cggcggcgat gccgatacac tggcccgctt
     6661 cagtcactgg aataccgaat tgaaggaaga atggaaagcc agtgccgata ttttcatcat
     6721 tcaatccaaa cgctacacac tctggaagga tttcctgaat ccgaatgagt ttgaagagta
     6781 ccccaaaccg cccgtggccc cctcttgcga ggatgggtct gaactaagga tatttcacag
     6841 gctgccatga atctttcact tgtagttcca gtctataacg agcaggataa cctgccgctt
     6901 ttgttcgaag ccattaacaa ggtcatgccc acgatgaatc gcacatggga ggtgattttg
     6961 gtggacgatg gcagtcagga taacagcctg gctgttttga acgagtacgc caaaaaagac
     7021 tttgctcatg tgcgtgtgat ctcattccgc cgcaacttcg ggcagaccgc cgcgattgcc
     7081 gccgggctcg attatgccca gggcgagatc atcgtgctgc tggatgcaga catgcagaac
     7141 gaccctgctg acatccccat gatgcttgcc aaactggacg aaggttatga ccttgtcagc
     7201 ggttggcgca agagccgcaa agataatgcc atcacccgca atttcccatc catgatcgcc
     7261 aaccgcatca tttcaaaggt gacgggggtg catctgcacg attacggctg cacactcaaa
     7321 gcctatcgcc gcgacgtgct tgaagggttc cgcttgtatg gcgaaatgca ccgctttatt
     7381 ccggtctatg cccattcggt cggggcgaag atcaccgagg tggtggtcaa ccatcacccg
     7441 cgcaaacatg gcaagaccaa gtacggcctg gagcggaccg caaaggtcgt gctcgatctg
     7501 tttaccgtaa aatttctgat ctcctattct tcgaaaccca tttatctgtt tggcggcgca
     7561 ggcgggtttc tcatggtcat cagcgccatc atcatgttgt atctttttgt gcgccgcatt
     7621 ttctttttgg ttgccattgc caattcgccg ctgttgcaga tgagcgcgat gttcttcatc
     7681 ctcggcttcc aatccatcct gctgggtctg atcgcggaac tgctcgtccg cacgtatcac
     7741 gagtcacagc gcaaacccac ctataccatc cgcaccatga tcaacctgga gcaggatccg
     7801 cgtgattaac tggctcagca agaaccgtca gaccgttgcg cgtgggatcg gcagcgttct
     7861 ggcgttggtg cttctggcgg tcttgattca ggagcagggg gatggtgaat tgttttccgc
     7921 cttccgccgc gtttcactgg ggtattttgg gctggctctt gtcgtcttgc tcgtttcgcg
     7981 gctttttgcc attttgcgct ggcacatcct gctgcgctcg gcgggtgtga acatttcctt
     8041 cctccgctcg gttgaactca cctttactgg caacttttcg tccaatttcc tgccgaccac
     8101 catcggcggg gatgtggtgc gtttgaccgg tgccatgcag cttggttatg accgcgccat
     8161 ttgcctcgcc tcactcgtcg tggaccgttt ggtggggatg gcgggaatgg cattggccct
     8221 tccctttggg ctggttccgc tttttgcgct ttcgaacggt tcgacccaat ctgtcacatt
     8281 atccgccctc cttcaaaaag gcgtggactt tatccgccgc actttcgaat cgctttcgat
     8341 ctggttgaag aaaccctcgg ccttgtttgc ttccctgctg gctacgctgg gtaatcaggc
     8401 ctttatttat ttgatggtct attttctttt gctgggcatc ggtcaccgcc tttcgttctg
     8461 gctggtggcg ggcatgtaca cactctctta tttcgtgacc ctgattccca tttccatcaa
     8521 cggcctcggt gttcaggaac tttcgatgac gtttttgctc accagcctcg gcgggctgac
     8581 cgaatcagaa agcgccacag tggccgtatt ggtgcgcatt attttcctca tcaccagcct
     8641 gcctggtgca tacttcctgc cctccatcct tgccgcaatg aatagcaagg aagttgacga
     8701 aagataaacc tgatatcggg gcggggaatt gatccgcccg atttttgaac gacgcaggtc
     8761 tgcttccgca atgaaaccaa cctcctcgaa aatcttctgg caaacattca tcaccgccac
     8821 agtcctcatg agcgggctgg ccgcctacca gaccgttcag cgcgtggctg atttggagat
     8881 caccctctgg cgatccaagt ggattctgct ggtgggcctg ttcgcgttga atatttttgc
     8941 ggggatgttt gtgctgtggc agctttccac tgcccgcgct tcatacctga ccgaaaaaca
     9001 ggccgccgtt acgaaaagcg gaatcaaccc gtgggctggg tctgtgttga ttgcctttgg
     9061 cttctttctc gtttgggcgg ttcgcctttt cttttttggg gatgtccttc cgcaaattgc
     9121 acccatcttc tgggtttttc tctgggcaag tttgattcaa acctttggct tgcaattgct
     9181 gacaggcagg gattggctca ccccgttcgt ggcggctgtg cttgtgcagg gggtggtcta
     9241 tcaaattttc gggcatctca ccatcgtttc tgcgtatcca ttttccatcg gttattctga
     9301 agcggggcgg cattattatg cttcgctgtt ctttgctaga ccgctgtacg gaaccgaact
     9361 cgcgcttcca ttcttgcacc ccacgcgtta ctttttgatg tcgcttccat ttctggtgga
     9421 cggtttgccg ctttgggcgc atcgcctctg gcaggcgctg ctgtggatcg gtctgactgc
     9481 cgcctcttcc attttgttga tccgccgctt gaacttcaca gggtgggttc gattcgcgct
     9541 cattgcctgg gccttcctgt attttttcca aggcgcggtt tattatcatt tgcaggtctg
     9601 tgtcattttg attttggcgg gggtgtcggt caagcacccg tggcggtcat tgctgttcgt
     9661 ggttctcgct tctgtttggg cgggtgccag ccgcgtgaac tggtttcccg tccctgccat
     9721 gctggcggtt gcgatctacc tgcttgaaac accgctggaa ggaaaaggct ggcgttattg
     9781 gctgaccccg ttcctgtggg gaatctgcgg gcttgctgcc gcatctgctt cgcagtttgc
     9841 gtacatcgcc gtttcaggca acagtgatgc gcgttccttc ggttccagtt tcacatctga
     9901 cctgatctgg agccgcctgc ttccgaatga aaccttcccg atgggaattt tattggggat
     9961 tgtcatcgtc tctgccccgt tgatctttgc gttcattcaa atggtccgcg gaaagatttc
    10021 caatttgcat ccgttgcgct ggctggcatt gattgccatg ctggctgttc tttttctcgg
    10081 cggcatggtc gtcagcgtga agatcggcgg cggcgcagat ttgcacaaca tggatgcgtt
    10141 tctcgtaatg ctggcgcttg tcgtgacggc tttcatcggc gggcgggtag cgggggagcg
    10201 cgaagcgaat cctgcctggg gggcgattcc tgtttgggtt gttgcagcag ccgccctcgt
    10261 cccgatcgga ttcgcgcttc cacaggttgg attctttcaa aggtacgatt caaaccttgt
    10321 cgaagcggac ctgttcaagt tgcaggcatc cacacaaaat attgaaggcg aaatcctttt
    10381 cgtaaccgaa cgtcagttgt tgattttcaa cgatgtaaat aatattccgc ttgtgcccga
    10441 atatgagcag atggaactga tggaaatggc gatgtcgggc aaccgcgact atcttgaaaa
    10501 tttttacgct gacctgagat cgcatcgttt cgcgctgatc gttgccgagg aacaaaaatt
    10561 tgcactgcaa aaacacggcg cgtttttgga agagaacaat gcgtgggtgc gttttgtggg
    10621 cgcaccgatc ctgtgcgcgt ataaacctgt gacgactcta tcctcgacaa atattcaagt
    10681 gtttgcgccg cgcgagaaca accccgaatg caaagacccg tttgaaaaat aaccatgcga
    10741 attttgctca tcaacagcga gtatccaccc atcggcggcg gcgcaggcaa tgccagcgcg
    10801 aacatcgccc gttgtcttgc cgcactcggg cacgaggtga cggtgttgac ctcgctgttc
    10861 ggcgaccagc cttttttgga aaccctcgac ggcgtcacga tt
//
LOCUS       DAOQLC010000133        10857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2351_length_10857_cov_23.195982, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000133 DAOQLC010000000
VERSION     DAOQLC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_10857_cov_23.195982"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..723)
                     /gene="nth"
                     /locus_tag="PL000_17795"
     CDS             complement(58..723)
                     /gene="nth"
                     /locus_tag="PL000_17795"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC90788.1"
                     /translation="MKNPKERALLIHQTLLTAFGEPIWRNPLPAIDELVSTILSQNTN
                     DINRDRAFDALRAKFPTWESVRDANAEDVIAAIKPAGLANQKGPRIQQVLRAITEERG
                     SLNLDFLAGLPIEEARSWLTKFNGVGPKTAAIVLCFSLNMPAFPVDTHVYRVTGRIGL
                     RPEKMTVEQAHPHLESVLPPETYYAAHLNIIRLGREICHARKPNCPQCPIVKLCEYKE
                     KTK"
     gene            complement(720..1445)
                     /locus_tag="PL000_17800"
     CDS             complement(720..1445)
                     /locus_tag="PL000_17800"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNC90789.1"
                     /translation="MQPGVLGLAVVGSYARGEHTAHSDIDLDIFVESLSGEADYTLTH
                     MEGRLVSLKYLTIAQELESLGKPQKAIWAVPGLRQMVILTDDTGAIANLKQAAQNFNW
                     QAMQPAADEYAAEELMSCAEEIHKIVHGLSADNESKVLYAVWGLFRGLANAVAVQQGM
                     MMTSENRYFDIIQQTIGATHPWTKSFRLALGADIIATQPAYITRGKAALDLYRHTAVL
                     FQNIIPEKHRDVIENTLQLLDTL"
     gene            complement(1490..2542)
                     /locus_tag="PL000_17805"
     CDS             complement(1490..2542)
                     /locus_tag="PL000_17805"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNC90790.1"
                     /translation="MRQPLLELTRGRVVESTHFGSIAIVDSNGKLLHSYGDPYEVAFL
                     RSSAKPFQALPFIERGGAEHFHYTQSEISISCASHETAQIHLDTVRAMQAKIGIQETD
                     LQCGGHLPGDVPVLKQVILQGILPTANFNNCSGKHTSMLAHAKMRGLPLENYLDRSHP
                     IQQDILTTVSEMCKFDRDKIELGIDGCSAPNFALPLFNAALGMARMCDPRELSEPRAT
                     ACQKITAAMIAHPEMVSNFGEFDCELMQACQGRVITKRGAEGFQLIGLMPGVYGEYGV
                     GIAFKVTDGDASRMNDELESSVRVRPAVTLEILRQLKAIQDAQLKSLQAFGPKKQLKN
                     HRGLVTGESYPAFQLA"
     gene            complement(2545..3402)
                     /locus_tag="PL000_17810"
     CDS             complement(2545..3402)
                     /locus_tag="PL000_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC90791.1"
                     /translation="MQEITNNVFIEDQFPGVTLGVIVTPRGLIQIDAPPSPEDARSWR
                     AALMNLGGGMERVLVNLDAHPDRTLGARAMDCTVIAHEKTANTFRTRPNTFKAQGEET
                     GADWESIAGLGSVRWAPPEISFLNQMTLHWSDSPVFLEHHPGPASGSIWVHLPNEKIL
                     FVGDAVLKNQPPFFAGSNLANWLTTLNLLLSPAYKGYTIISSRGGVVTANIVKAQLDF
                     IKHTHDKLEKSTTKKPNPAATEKLVTSLLTWFKAPAARQKQFAQRLRYGLLHYNARPN
                     HTAGQYNED"
     gene            complement(3427..5559)
                     /locus_tag="PL000_17815"
     CDS             complement(3427..5559)
                     /locus_tag="PL000_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90792.1"
                     /translation="MTEDRSRSLLELLVNISREVATALDLRTVLQRLLFAALQYVGGE
                     RGSIVVMDDLGKPVDATIVYGKQLHEHTTQQLRDTVERGLAGWVIQRRKAALVPDTSK
                     DERWLRREDDSVDRSGAKSALCVPLMARERLVGVLTLVHSIPNAFTEEHLDLMRAIAD
                     QASVAVLNARLYTESQRTARVMSSLAEGAAAINTSLELPDVWRRILNQTMQALQVETV
                     ALALIDNTTDDIVFQAAAGHNSGNIPGRHIKNGRGLTGLVVSNGFGLIVPNVNADERF
                     SEVDKFGGIVMRALAIVPIQTQGKVIGILEAINPISGAFDMDALLVLTGLGSLAGTTI
                     QNAQFMERLKKAHERYRELFEDSVDPILITDWEGRVLEANRQAVSLSGYTNEQLHNMS
                     IDQLHEVKWNNTGLNFETLKHNDECNYESDLHQQNGGVVPIEVHAHRVEIEESDSVQW
                     ILRDITERKELDALRKDMTDMIYHDIRSPLGNIVSSLEMMKDMLPENDETLFTMLNIA
                     RSSAGRIQRLVYSLLDTNRLESGQPIVDQHAIDPVALIRESVRDTERGVENREQTLEN
                     KANTVLPLIWVDVDMIYRVFINLIENAIKYTPMGGHIEVGAQTTDGMFVKFWVKDNGP
                     GIPPQDRDRVFDKFTRLRGKNNNPSGIGIGLSFCKLAVQGHGGKIWVESEVDKGTTFF
                     LTLPVAQKKATGQLKRQTGRLTIKEDKL"
     gene            5642..6304
                     /locus_tag="PL000_17820"
     CDS             5642..6304
                     /locus_tag="PL000_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC90793.1"
                     /translation="MSLDASRIKALCFDVDGTLSDTDDLYAQKVERLFPRFLFKNPAR
                     AARRFVMWVEAPGNALLGYADRLGLDDEMVAIINWISRHRKHSDKKFLLVPGVDDMLK
                     QLHGRYPMAVVSARDERGTLAFLEQFGLVKYFDAIVTGLSAEHTKPYPDPVLLAARKM
                     NVPAENCLMIGDTTVDIRAGKSAGAQTVGVLCGFGEEPELKKLGADLILSDTPKLLDV
                     LK"
     gene            complement(6413..7564)
                     /locus_tag="PL000_17825"
     CDS             complement(6413..7564)
                     /locus_tag="PL000_17825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC90794.1"
                     /translation="MASEERFNELDGMRGIATILVISYHIFKRGDYFTTNAVLHFITS
                     LTLYGWYALDTFFVLSGFLITGILLRTKEEKDYFKNFYVRRSLRVFPLYYFVLALILF
                     LMPKLDPDYVAEIPKALPYYLFYQQNWFHFLTNVKGTEHLSVTWSLAIEEQFYLLFPF
                     LVYFTRKETLAKVAGGIIFVSILARVLSAFLWTDVDQMTNFYFYNTFTRFEEISFGIL
                     IAVAFTYPDWKAKLSRIAMPVFLIAYPALLITEAVTSRSIGIPHPAYGNVPLTVVGYT
                     LASVFAAALIVVLTTHDRMSFIRRFFRNKVLTFFGDHSYSIYLFHMPIGLTLLDYLWR
                     NDYRGWQIYFLYIGLTFGITIFISILTWNFLEKPMLGLKKYFEYKEPAK"
     gene            7691..9508
                     /locus_tag="PL000_17830"
     CDS             7691..9508
                     /locus_tag="PL000_17830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90795.1"
                     /translation="MALISLQEVSIGFGGPPLLDKVNLQIEQGEWIGLLGRNGMGKST
                     LLKLVNDDIAPQSGIIARQQDLRTAYLPQEVPAGITGKVFDIVAGGLDSLKPVPASDE
                     HHWRGQLQVEQVISRMQLDGEARFEILSAGMKRRVMLARGLVRQPELLLLDEPTNHLD
                     IASIDWLETFLKRWGGTLLFVTHDRVFLQKLTSRIVELDRGRLFDWNCDYSTFLERRD
                     AMLSAEAEQNALFDKKLAQEEVWIRKGIEARRTRNEGRVRALKRLREQRKERRERPGK
                     VKMQIQSEARSGKIVIEAENVSYAYDGRTIVKEFTTTIQRGDKIGIIGPNGSGKTTLL
                     RLLLEELTPQTGTVEHGTNIEIGYFDQLRAQLDETKSVLDNVGQGSDNVTINGRSRNL
                     NVYLEDFLFSRDRINAPISALSGGERNRLMLARLFAQPANLLVMDEPTNDLDVETLEI
                     LEELLLDYEGTLLLVSHDRAFLNNIVTSTLALDGSGRVIETVGGYDDWAKQNESAPIE
                     SKPRKAAVGLENEARSSSSKKPSYKEQRAIEAQKRELAELPQRIEKLEADLHALTAEM
                     ATPNFYQRDSSEIAQTAERLKQMQDELNQAYARWEELET"
     gene            9542..9982
                     /locus_tag="PL000_17835"
     CDS             9542..9982
                     /locus_tag="PL000_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC90796.1"
                     /translation="MQFTKDELAALLNGEKPLQLTGLDLSKANLIRAELSKANLSGAD
                     LNGAFLDEARLRYANLSEANLEGAHMNRANFHGANLADADLSDSDLSEADLSGANLAD
                     ATLMYANLTSANLSGANLNGAKYNHHTQWPAGVDPKAAGAVLAE"
     gene            complement(10184..>10857)
                     /locus_tag="PL000_17840"
     CDS             complement(10184..>10857)
                     /locus_tag="PL000_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90797.1"
                     /translation="VKSMLGYLPQELGMYTELTAEQFVDYMAILKGLDDSRKRNQRVA
                     EVLEMVGLREVAKRKIKGFSGGMKRRVGIAQALVNDPKMLIVDEPTAGLDPEERIRFR
                     NLLVNLAADRVVLLSTHIVEDIGQTCRDLAVLAKGRILFRGSPAEMLKATEGHVWTLT
                     MSGLEKPNHDLTVVSMLHLAEGTQYRLVGANVNGYSNAQSTQPGLEDGYVWLMKNSDN
                     LVSVE"
BASE COUNT         2528 a         2689 c         3035 g         2605 t
ORIGIN      
        1 ttgtgtgcgg cacaccaagt tgttccaaag caatactgac tgggggagtt tccatgttta
       61 tttggtcttc tctttatatt cacacagttt gacgatggga cactgtggac aattcggctt
      121 gcgcgcatgg cagatttcgc gcccgaggcg gatgatgttc aaatgcgcgg cgtaataggt
      181 ttcaggcgga agcacagatt cgagatgcgg gtgggcctgt tccacggtca ttttttcggg
      241 gcgcagaccg atgcggcctg taacgcgata gacgtgggta tccactggaa aagcgggcat
      301 gttcagcgaa aaacataaaa cgatagcggc agtcttcggg ccgacgccgt tgaacttcgt
      361 cagccaggag cgtgcctctt cgatgggcag accagctaaa aagtccagat tgagcgagcc
      421 gcgttcttct gtgatggcgc gtaagacctg ttgaatgcgc gggccttttt gattcgccaa
      481 tcctgcgggt ttgatcgcgg cgatcacatc ctccgcgttt gcatcgcgga cagattccca
      541 ggtggggaat tttgcgcgca gggcatcgaa ggcgcggtcg cggttgatgt catttgtatt
      601 ttgcgagagg atggtggaga cgagctcatc tatggcaggg agcgggttgc gccaaatggg
      661 ctcgccgaat gccgtgagca gagtttggtg gatgaggagc gcgcgttctt ttggattttt
      721 cataatgtgt cgagcaattg tagcgtattc tcaattacgt cgcgatgttt ttcagggatg
      781 atattttgaa aaagcacggc agtgtgcctg taaagatcga gggcggcttt tccacgggtg
      841 atgtaggctg gctgggttgc gatgatatct gcaccaagcg cgagacggaa ggattttgtc
      901 cacgggtgag tggcgccgat ggtttgctgg atgatatcga agtagcggtt ttcgctggtc
      961 atcatcattc cttgttgaac ggcgaccgca ttggccaatc ctctgaacag cccccacaca
     1021 gcatacaaga cctttgactc attatccgct gaaagcccgt ggacgatctt gtgtatttcc
     1081 tcagcgcaac tcattaattc ctcggcggcg tattcatctg cggcgggttg catggcctgc
     1141 caattgaaat tttgtgcagc ttgtttgagg tttgcgattg cgcccgtatc gtcggtgagt
     1201 atgaccattt gcctcagccc cggtacagcc cagatcgctt tttgtggttt gccaagcgat
     1261 tccagttcct gtgcgatggt gagatatttc aaactgacaa ggcgaccttc catgtgggtg
     1321 agggtgtaat cggcttcacc cgaaagcgac tcgacgaaga tatccaggtc aatatcgctg
     1381 tgggcagtgt gttcgccccg tgcatagctg ccaaccactg caagaccaag aacgcccggt
     1441 tgcattagcc gggcgctcag agatttgagg aatgggtctt cgtggaggtt catgcgagtt
     1501 ggaaggcggg gtacgattcg cctgtgacca gtccgcggtg atttttgagt tgttttttgg
     1561 ggccgaaggc ttgcagagat ttcagttgtg cgtcttgaat ggctttcaat tgacgcagga
     1621 tttccagcgt aactgcaggc cgcacacgca cactggattc aagctcgtcg ttcatgcggg
     1681 aggcatcgcc atcggtgacc ttgaaggcaa taccaacgcc gtattcgcca tacacgccgg
     1741 gcatcaggcc gatgagttgg aagccttccg cgccgcgctt ggtaatgacc cgtccctggc
     1801 aggcttgcat caattcacaa tcgaactcgc cgaagttgct caccatttca gggtgagcga
     1861 tcatggcggc tgtgatcttt tgacaggccg ttgcgcgggg ttcgctcaat tcacgcggat
     1921 cacacatccg tgccatgccg agggctgcgt tgaacaaggg cagggcaaaa ttgggcgcag
     1981 aacagccgtc aatgccaagc tcgatcttgt cacggtcaaa tttgcacatc tctgaaacgg
     2041 ttgtcagaat gtcctgctgg atgggatggc ttctatcgag ataattttcc agtggcagtc
     2101 cgcgcatttt ggcgtgagca agcatggagg tgtgcttgcc agagcagtta ttgaaattag
     2161 cggtgggcag gattccctgc aggataacct gcttcaatac aggcacatcc ccgggcaggt
     2221 gtccgccgca ttgcaggtcg gtttcctgaa tgccgatctt ggcctgcatg gctctgaccg
     2281 tatcgagatg aatctgtgcg gtttcgtgcg aagcgcatga gattgatatc tcgctttgtg
     2341 tgtagtggaa atgttccgct ccgccgcgct caatgaatgg cagtgcctga aacggtttgg
     2401 ctgatgaacg taaaaaggcg acctcgtatg ggtcgccgta ggagtgcaga agtttaccgt
     2461 tcgaatccac gatggcgatg gaaccaaagt gggttgattc gaccacgcgc ccgcgggtca
     2521 gttcaagcag gggttggcgc atgtttaatc ctcattgtat tgcccggcgg tgtgatttgg
     2581 gcgggcgttg tagtgaagca acccatagcg caggcgttgg gcgaattgtt tctggcgggc
     2641 ggccggggcc ttgaaccatg tcaggagtga cgtaaccagt ttctcggtcg ccgctggatt
     2701 cggttttttc gtcgtgctct tttccagctt gtcgtgtgtg tgtttgatga aatcgagttg
     2761 cgcttttacg atgttggccg tcaccacacc gccgcggctg ctgatgatgg tatagccttt
     2821 gtaggccggg ctcagaagca ggttgagggt ggtaagccag ttggcaaggt ttgaacccgc
     2881 aaaaaaaggc ggctggtttt tcaagacagc atccccgacg aacaatattt tttcattggg
     2941 gaggtgtacc caaatggaac cgctggctgg cccggggtga tgttccaaaa atacggggga
     3001 atcgctccaa tgcagggtca tttgatttaa aaaggaaatt tcaggaggtg cccaacggac
     3061 acttcccaac ccggcaatgg attcccaatc tgcgcctgtt tcttcccctt gagccttgaa
     3121 ggtgttcggg cgggttcgaa aggtgttggc ggttttttcg tgggcgatca ctgtgcaatc
     3181 catggcacgt gcgccgaggg tgcggtcggg gtgtgcatcg aggttgacga gaacccgttc
     3241 catgccgccg ccgagattca tcaaagccgc gcgccatgag cgtgcgtctt cgggggaggg
     3301 gggtgcatcg atctggatca atccgcgggg tgtgacgatc acgccgagtg taaccccggg
     3361 aaattgatct tcgatgaaaa cgttattggt aatttcctgc atgctgctcc tgaaaaaaat
     3421 ggaaaattaa agcttgtctt ccttgatggt caaacggcct gtctgacgtt tgagctggcc
     3481 tgtggctttc ttttgggcaa ccggcagggt gaggaagaag gtggtgcctt tatcaacttc
     3541 actttcaacc cagatctttc caccatgtcc ctgcactgcc agcttacaga aagacagccc
     3601 aatgccgatg ccgctggggt tgttattttt gccgcgcaga cgggtgaatt tatcgaagac
     3661 gcggtcgcgg tcctgcgggg gaatgccggg cccattgtct ttcacccaaa acttgacgaa
     3721 cattccatcg gtggtctgcg cgccgacttc aatatgcccg cccatgggcg tgtacttgat
     3781 ggcattctcg atcaggttga tgaatacgcg gtagatcata tccacatcca cccaaatgag
     3841 cgggaggacg gtgttggcct tgttttccag agtctgctcg cggttttcca cgccgcgttc
     3901 ggtgtcgcgg accgattcgc gaatcagcgc caccgggtca atggcgtgct gatcaacgat
     3961 cggctgacct gattcgaggc ggtttgtgtc gagtaacgaa tacacaagcc tttgaatgcg
     4021 ccccgcagaa ctgcgtgcga tgttgagcat ggtgaaaagg gtctcgtcgt tctcaggcag
     4081 catatccttc atcatctcaa ggcttgaaac gatgttgccg agcggcgagc ggatatcgtg
     4141 atagatcatg tcggtcatgt ctttgcgcag ggcatccagt tccttgcgtt cggtgatgtc
     4201 acgcagtatc cactgaaccg agtcgctttc ttcgatctcc acacgatgag catgaacttc
     4261 aatggggaca accccgccat tctgctggtg caggtctgat tcgtaattgc attcatcgtt
     4321 atgcttgagg gtttcaaagt tgagaccggt gttgttccac ttgacctcat gcagctggtc
     4381 aatgctcatg ttatgcaact gctcattggt gtaaccactc aggcttaccg cctgacgatt
     4441 ggcttccagt acacggcctt cccaatctgt gatcaagatc gggtcaacac tgtcttcaaa
     4501 cagttcgcgg taacgttcat gggccttctt caatcgctcc atgaactggg cgttttggat
     4561 ggttgtgcct gcaaggctgc cgagaccggt caacaccagc agcgcgtcca tgtcgaatgc
     4621 gccagagatg ggattgatgg cctcaaggat gccgatgacc tttccctgag tctggatcgg
     4681 gacgatggcc aaggcccgca tcacgattcc cccgaatttg tccacttcac taaagcgttc
     4741 gtctgcgttg acgttgggta cgatcaaccc aaacccattg ctgaccacca acccagtcaa
     4801 cccgcggcca tttttgatgt gcctgccggg gatattgcct gagttgtgac ctgctgcagc
     4861 ttgaaaaacg atgtcgtcgg tggtgttgtc gatcaaagcc agtgccacgg tctcgacctg
     4921 taaggcctgc atggtctggt taaggatgcg gcgccacaca tcgggcaatt ccagcgaagt
     4981 gttgatcgcc gccgccccct ctgccagtga ggacatgaca cgggcggtcc gctggctttc
     5041 ggtgtagagg cgcgcgttca aaacggccac actggcctga tcggcgatgg ctcgcatcag
     5101 gtccagatgt tcttcagtaa aagcattggg aattgagtgg acgagggtca acacacccac
     5161 cagacgttca cgtgccatca acggcacaca caacgccgat tttgccccgc ttctatccac
     5221 agagtcatct tcacgacgca gccagcgttc atccttgctg gtatcaggta ccagtgcagc
     5281 tttgcggcgc tggatcaccc agcctgcaag accgcgttcc actgtgtcac gcaattgctg
     5341 ggtggtatgt tcatggagtt gtttgccgta gacaatggtg gcatccactg gtttgccaag
     5401 atcatccatg acaacgatgc tgccgcgttc gccgcccaca tattgaaggg ctgcaaaaag
     5461 caaccgctgt agtaccgtgc gaaggtccaa cgcggttgcg acctcacggc tgatgttgac
     5521 caataattca agcaatgaac gagagcgatc ttcagtcata aatatttgtt aaattatact
     5581 acgcaaacct tttcgaatta ccctgaagga cggagggagc cgttttggta caattgcgcg
     5641 catgtcactt gatgcttccc gtattaaagc cttatgtttc gacgtggatg gcaccctgag
     5701 cgataccgat gacctgtatg ctcaaaaggt ggagcggctt ttcccccgct ttttattcaa
     5761 aaaccctgct cgtgcggcgc ggcgctttgt catgtgggtg gaagcacctg gcaatgccct
     5821 gcttggttac gcggacaggc tgggtcttga cgatgaaatg gtcgcgatta tcaattggat
     5881 ttcccgccat cgcaaacatt cggataaaaa attcctgctc gtaccaggtg tggatgacat
     5941 gttgaagcag ctgcatggac gttacccaat ggctgtggtc agcgcacggg atgaacgcgg
     6001 cacgctggct tttctcgaac aattcgggct ggtgaaatat ttcgatgcca tcgtaacagg
     6061 cctgtctgca gaacatacca agccgtatcc agaccctgtt ttgttggcgg cacggaaaat
     6121 gaatgttccc gcagaaaatt gcctgatgat cggcgatact acagtggata tccgtgcggg
     6181 gaagtccgcg ggtgcgcaga ccgttggtgt gctttgcggg tttggggagg aacctgaact
     6241 gaaaaaactt ggtgcggatc tgatcctttc agatacgccg aaactgttgg atgtgctaaa
     6301 gtgacctgtt gtgtcatgaa gtaagcaaaa tcaacatcaa aaacataact caccccgaaa
     6361 ttcagcttcc gtgggtgagt tatgttttta aacctctggc agcaatggct gtttactttg
     6421 cgggctcttt gtattcaaaa tactttttca ggccaagcat cggcttttca aggaaattcc
     6481 aggtcagtat ggaaatgaag atggtgattc caaaagtcag accgatgtag agaaaataga
     6541 tctgccagcc ccgatagtca ttccgccaca aataatcgag cagggtgagg ccgatgggca
     6601 tgtggaagag atagatcgaa taactgtggt cgccgaaaaa ggtcaacacc ttgttgcgga
     6661 aaaatcggcg gataaaactc atcctgtcat gggtggtcaa caccacaatg agcgccgccg
     6721 caaaaacgga agccagtgtg tacccaacga cggtcagcgg cacattgccg taggcggggt
     6781 ggggaatgcc aatacttcgg ctggtgaccg cttctgtgat aagcaaagca gggtaggcga
     6841 tcaaaaagac aggcatggcg atcctgctga gtttggcctt ccaatcgggg taggtaaatg
     6901 caacggcgat caaaatccca aatgagatct cttcaaaacg ggtgaatgtg ttgtagaaat
     6961 aaaagttcgt catctggtca acatccgtcc acaaaaaggc ggaaagcacg cgcgccagaa
     7021 tggaaacgaa aatgattccc cccgccacct ttgccagtgt ttccttgcgc gtaaaataga
     7081 ccagaaaagg gaacagcagg taaaactgtt cttcaatggc cagtgaccag gtcaccgaga
     7141 gatgctccgt gcctttcacg ttcgtgagaa agtggaacca gttctgttga tagaaaagat
     7201 aataaggcag cgccttcgga atctcagcca cgtaatcggg gtcgagtttg ggcatcagaa
     7261 acaggatcaa agcgaggaca aaataataaa gaggaaagac ccgcagggaa cggcggacat
     7321 aaaagttctt aaaataatct ttctcttcct tcgtcctcaa caggatccca gtgatgagaa
     7381 aacctgaaag gacaaaaaaa gtatccagcg cataccatcc atacagggtc aggctggtga
     7441 tgaagtgcag taccgcattt gtcgtgaaat aatcgccccg tttgaaaata tgataggaga
     7501 tgaccaggat cgttgcgatt ccacgcatgc catccagttc attgaaacgt tcttctgacg
     7561 ccatggaagc atccttttcg ttcacaggaa taataaggtg cgtaattgta ctatcgttta
     7621 ttttctgaat agtaaacccg cggggtttgg tatttcgtag tatattctgc cccctacgag
     7681 gaaaaatttc atggcattga tcagtttaca ggaagtcagt atcgggtttg gcggtccgcc
     7741 gctgcttgat aaagttaatc tacagatcga gcagggagaa tggatcggct tattgggccg
     7801 caacggcatg ggaaaatcca ccctgctgaa actggtcaac gatgatattg ccccacaaag
     7861 cggcatcatc gcccgccaac aggatttgcg caccgcctat cttccgcagg aagttcctgc
     7921 tgggatcaca ggaaaggtgt tcgacattgt tgcaggcggt ctggactcac tgaaacctgt
     7981 ccctgcctcc gacgaacatc actggcgcgg acagcttcaa gtggaacagg tcatctcgcg
     8041 gatgcagttg gatggcgagg cccgttttga gatcctctcc gctgggatga aacgccgtgt
     8101 catgcttgcc cgtggattgg tacgccagcc cgaactgctt ctgctcgatg aacccaccaa
     8161 ccacctcgat atcgcctcca ttgactggct tgaaaccttt ctcaaacgct ggggcggaac
     8221 cttgctgttc gtcacccacg accgcgtctt tttacaaaaa ctcacctccc gcattgtcga
     8281 attggaccgt ggacgcctgt tcgattggaa ttgcgattac tccaccttcc tcgaacgccg
     8341 tgatgccatg ctttctgcag aagccgagca aaacgcactc ttcgacaaaa aacttgccca
     8401 ggaagaagtt tggattcgca aagggatcga agcccgccgt acacgcaacg aaggccgtgt
     8461 gcgtgcgctc aaacgtctgc gcgaacaacg caaggaacgc cgcgaacgac cgggcaaagt
     8521 gaaaatgcag atccagagcg aagcccgttc gggtaagatc gtcatcgaag cggaaaacgt
     8581 cagctatgcc tacgatggac ggaccatcgt caaagagttc accaccacca ttcaacgcgg
     8641 cgacaagatc ggcatcatcg gcccgaacgg ttctggaaaa acgactttgc tgcgcctgct
     8701 tttggaagaa ttgaccccgc aaacggggac ggtggaacac ggcacgaata tcgagatcgg
     8761 ttacttcgac caactccgcg cccaattgga tgagaccaag tcagtgctcg ataatgtggg
     8821 gcagggcagt gacaatgtca ccatcaatgg ccgttcgcgc aacctgaatg tgtatctgga
     8881 ggattttctc ttttcacgtg accgcatcaa cgcgccgatc agcgcgctct ccggcggcga
     8941 gcggaatcgt ttgatgctgg cgcgtctatt tgcccagcct gccaacctgc ttgtgatgga
     9001 tgagcccacc aacgatctgg acgtggaaac attggagatc ctcgaagaac ttttgctcga
     9061 ttatgaaggc acgctgttac tcgtcagtca tgaccgtgct tttctgaata acattgtcac
     9121 cagcacactg gcccttgatg gcagcggacg ggtgatcgaa acggttggtg gatacgacga
     9181 ttgggcgaag caaaatgaat cggcgccgat cgagtcgaag ccgcgcaagg cagctgtcgg
     9241 cttggagaat gaggcgcgct cttcctcctc caaaaaaccg agttacaagg aacagcgtgc
     9301 gatcgaagct caaaaacggg aacttgccga acttccacag cggatcgaaa aactggaagc
     9361 ggatcttcac gcactgacgg ctgagatggc gaccccgaat ttttatcagc gggatagttc
     9421 tgagatcgcg cagacagcgg agcggctgaa gcaaatgcag gatgaactca accaggcgta
     9481 tgcgcgttgg gaagaattgg aaacgtagta gtattgaagt aaattatgga aaggcgatac
     9541 catgcaattc acaaaagatg aacttgccgc attgttgaat ggagagaagc cgctgcaatt
     9601 gacaggtctt gacttgagca aggccaatct gatcagggct gagttgagca aggccaacct
     9661 gagcggcgcc gacttgaacg gagccttttt ggatgaagcc agattgcgct atgccaacct
     9721 gagcgaagcc aatttggaag gcgcccatat gaatcgggcg aattttcacg gggccaattt
     9781 ggctgatgcc gatctcagcg acagtgattt gagtgaggct gacctgagcg gggcgaatct
     9841 ggccgatgca actttgatgt atgccaacct gacctcggcc aacctgagcg gtgcaaattt
     9901 gaacggcgcg aaatacaatc accacacgca atggcctgcc ggggttgacc caaaagcggc
     9961 tggtgcagtg cttgctgaat aaaaataaac ttgcaggaca aaagaacgaa cctcgaaagt
    10021 aaagtttcga ggttcgttcg tcttaaggca aagtggggga tctgtttgtg aaatacacag
    10081 aacatactgg cttctgataa aacttccaca atgggttctg aaatcctttt tagggattta
    10141 tcccgatgtg attcgtgatt tgtaaatatt tttgttttgt ccgctattcc acactgacca
    10201 gattatcaga gtttttcatc aaccacacat agccatcttc caaaccaggt tgtgtggact
    10261 gtgcattcga atacccgttg acatttgcac cgaccagtcg atactgagtc ccttccgcaa
    10321 gatgcagcat ggagacgaca gtcaggtcgt ggttcggttt ttccaaacca gacatagtca
    10381 atgtccagac gtggccttcg gtggctttga gcatttcagc gggactgccg cgaaagagaa
    10441 ttcttccttt tgcaagcaca gccagatcgc ggcaggtctg accgatgtct tcgacgatgt
    10501 gtgtggaaag caggacgaca cgatctgcgg cgaggttgac cagtaaattg cggaagcgaa
    10561 tccgctcttc ggggtcaagc cccgctgttg gctcgtcaac gatgagcatt tttgggtcgt
    10621 tgaccaaggc ttgagcgatt ccgacgcggc gtttcatccc gccagagaag ccttttattt
    10681 tgcgcttcgc cacttcgcgc agaccgacca tctccagcac ttctgccacg cgttgattgc
    10741 gtttgcggga gtcgtccaag cctttgagga tggccatgta atccacgaat tgttcggcgg
    10801 tcagttcggt gtacatgcca agttcctgcg gaagatagcc aagcatggac ttgaccg
//
LOCUS       DAOQLC010000134        10773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2366_length_10773_cov_21.562885, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000134 DAOQLC010000000
VERSION     DAOQLC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2366_length_10773_cov_21.562885"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..1941
                     /locus_tag="PL000_17845"
     CDS             259..1941
                     /locus_tag="PL000_17845"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC90798.1"
                     /translation="MSDVYDLKSVKLPYLAGGALKLFAALVEGPLGGLLTPSLFESAG
                     VNWLRKQSFNDAPTHHPIHFTGELQTESSAVPENEWPQNASTTKGFQFKSVHELANAY
                     RSGKTTPEEVARKVLDAIKASDSTDLPLKAFIAVYNEDVMRQARQSTQRIKAGKALSI
                     FDGIPVAIKDEMDMAPYPTTVGTSFLGKNPAQEDATVVARLRNAGALLIGKANMHEIG
                     INVTGLNPHFGTTRNPYSPRHYTGGSSSGSATAVAAGLVSFAIGADGGGSIRIPASFC
                     GLVGLKATFGRISEYGAYPLDWSVAHIGPLAASASDAALAYALIAGPDPKDPNSLHQP
                     LPSLQGWDDLNLKGLKLGVYWPWFRHADAETVAACEAMLKEYEKMGCEIVEVTIPNLE
                     ANRIAHSVTITSEMAQAMNETYGKHHRDHGLDVRVNLALARQFSATDYLTSQRIRTRM
                     IQHFNDAFQLADVILTPTTGITAPEIKKGALPDGESDLTNTIEIMRFATAANMTGLPA
                     ITFPVGYTQKGLPIGLQVMGKAWDEKTLLRLALSAEQVVERKAPQVYFELLK"
     gene            2008..2415
                     /locus_tag="PL000_17850"
     CDS             2008..2415
                     /locus_tag="PL000_17850"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC90799.1"
                     /translation="MAELKTKKTEASVEDFLNGVKDEATKKDCFEIVKLMKQATKADP
                     KMWGSSIVGFGTQHLKYASGRELEWMILGFSPRKQNLTLYLPGSLESYAGMLKKLGKH
                     TTGKGCLYIKSLKDVDTKVLKELVQQAVKTTKQ"
     gene            2412..2708
                     /locus_tag="PL000_17855"
     CDS             2412..2708
                     /locus_tag="PL000_17855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC90800.1"
                     /translation="MKPLKDPEEFEKKALHKFVDFSNAHVLEIGCGEGRLTWKYAAAS
                     TLTVGVDPDQNALRIARADTPYSLQEKVRFSRATARQLPFSKETFDIAVLAWSL"
     gene            2727..3149
                     /locus_tag="PL000_17860"
     CDS             2727..3149
                     /locus_tag="PL000_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90801.1"
                     /translation="MVHALDEIRRTLKPNGILIDLRPVEDHWSVEVASQQNILTAGQL
                     TDMPIGKADDEAAFRAMREVEARGWFTREREEEFSFFYYWDTPSEMKEFMEKEWKDFE
                     KMEDKVYQTAKSLWSTADADAQMRVRVKMLITKWRNTT"
     gene            complement(3162..4526)
                     /locus_tag="PL000_17865"
     CDS             complement(3162..4526)
                     /locus_tag="PL000_17865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNC90802.1"
                     /translation="MSLSFLRDRSFLREMLTIALPISFQQLINASLNLIDVLMVGQLG
                     ETSIAAMGLSNQVFFVFILLLFGLTSGMAIFTAQFWGKQEIEPIRKVLGMSILVSTLI
                     ALLFSLAATLMPQTVLGFYTADPEVIDIGTRYLRIVGLSYIPVALATSYIATLRSIQF
                     VKITVVATTTALIFKTILGYMLIFGYGPVPALGVEGAAIGTASGWTLELVLLLVMVYT
                     QKTPLAANPLTFFSFDLSFFTRVLKTTLPALANEMFWSLGITTYNAIYAHIGTDAIAA
                     INVNATIEELGFVVFMGLGNACAVMVGNRIGAGKKEEAYETVRRIVILGVSAAWIVGI
                     IVVLMRDVVTNLYDLSPSGVHNVRMLMLMMACTLWLRMFNFSTFIGALRAGGDTRFAL
                     IMEICSIWLIGVPVAYTSAFVFNLPVYYVYLMVGLEELAKAFVSGWRVRSRKWIHDLV
                     NETS"
     gene            complement(4625..5557)
                     /locus_tag="PL000_17870"
     CDS             complement(4625..5557)
                     /locus_tag="PL000_17870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC90803.1"
                     /translation="MKITYSIIAPIYNEIDNLPELYRRVKEVMDSSGEPWELILVDDG
                     STDGSTDGIRALAEKDKTVRPVIFARNFGHQVAITAGWDYARGDAVVIIDADLQDPPE
                     VILELAKKWKEGYEVVYAVRGEREGETWFKKFTAAMFYRIIYSITDVKIPVDTGDFRL
                     MDRKVVNVLKQMKERHRFPRGMSAWVGFKQIGVTYKRAARVAGVTKYPFKKMLRLALN
                     AITGFSYFPLQVATYFGFISAGISILAIPVVAILRLAGSHFFEGQTTTLVSVLFLGGV
                     QLISLGILGEYIGRLYDEAKGRPLYIVRDAPEDQ"
     gene            complement(5554..6723)
                     /locus_tag="PL000_17875"
     CDS             complement(5554..6723)
                     /locus_tag="PL000_17875"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC90804.1"
                     /translation="MKKKISQWSLPFRYAMGFVVFIAIVALLIYAREAVKMLVIAGFV
                     SYLISPAVILLMQKTKLSRTAAVNIVYFSALIFLVGVPATLTPIFFDEIKIVAQDILD
                     LSAEFSAVLSSPIQFGGMSFHFEQLGESLAHLQDTVLTPLPEEALALLETTSVNVLWF
                     LVIIVSVHLFMSQWPSIRDWLVDLAPEEYHHDMRELYKRLRNVWMAYLRGQIVLMLVV
                     GIVFTIAWLILGIPGALVLGVMAGLFTLVPDVGPFLAAAFAVGVALLEGSSWIPYSNV
                     IVAIIVFATYLVLINLKNFFVRPLIMGRSLHMNEALIFIAIMIATILEGIMGALLVVP
                     VLASVVVIMEYVRRRVLGLPPFEDDGEKRFVAPPEKIKARRPIKISTKEKRKEDL"
     gene            complement(6720..7988)
                     /gene="thrC"
                     /locus_tag="PL000_17880"
     CDS             complement(6720..7988)
                     /gene="thrC"
                     /locus_tag="PL000_17880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC90805.1"
                     /translation="MKNFTGYKCSLCGTEYLPGQVTYTCPKDGGNLDVVIDFDSIRKK
                     YQPEDITSRTEPSLWKYLPLLPVSEPAGDSTPLHAAGGTPVFALPRLAEKLGLKHLWL
                     KDESRNPSASFKDRASAILVARAQEIKAEVVVTASTGNAGAALACMAAAVGQKAVIFA
                     PKSAPPAKVAQLLVFGAKVILVDGTYDDAFDLTVKAADEFGWYCRNTGYNPFTVEGKK
                     TAAFEIWEWWMEAHRDWHKKDSPLDNHPPLCIFVSVGDGNIISGIHKGFKDLLALGWI
                     PNMPRIIGVQAEGSAAIANAFNANTETITPVSATTLADSISVDLPRDGVRAVRAAKET
                     DGTYITVADNEIIKSIAELGKMGVFAEPAGATAYAGLVKATSLGVVGSDDPILVMNTG
                     SGLKDVRAAMQAVQSAPIIEPTLEAVKKLL"
     gene            complement(8085..>8774)
                     /locus_tag="PL000_17885"
     CDS             complement(8085..>8774)
                     /locus_tag="PL000_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC90806.1"
                     /translation="ASRYAAKTLREEITASMAEKFDVRPEQIHFEDGIVHVNGHSLSY
                     ADVYKEMTSMGQQPKVRYEYEAPKTQPLGTGGDMHFAFSFGVQAAEVEVNKLTGEVKV
                     LKVISANDVGMAVNPLGLQGQVEGGVMMGLGNCLTEEFIVENGNVVTDQLARYRVPGI
                     MLTPEITAIIVEHPISSGPFGAKGVGEISSIPTTPAITNAIYNAVGVRFDKLPVDQEV
                     IARELWEREEK"
     assembly_gap    8775..8874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8875..>10773)
                     /locus_tag="PL000_17890"
     CDS             complement(<8875..>10773)
                     /locus_tag="PL000_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC90807.1"
                     /translation="QEAFVEHGAVQCGFCIPGQIMTAYALLNRNLDPNNDDIRFALKD
                     TLCRCAGYPSIENAIIAAAQALRTGEPVQKPTHIPDSVHEHKTVGHKHLRPEAVEKVT
                     GEAIYTDDLKFDGMLYAKAKRAMIPHGFLKKLDTSKAKALKGVIAVLTAEDVPAEKNH
                     GLVIYDWPVMVGIGERVRYVGDALAIVAAESPELAEQASALIEAEFDLQPVITNAVMA
                     REEGVPQLHEKGNLLKHIKVRKGDMDAGFAEADIVIEHTFHTPATDHAFIEPECSIAV
                     PLPDGRMEIYVGSQIPYQDRTQVARVMGWPEERVRIVGQLMGGGFGGKEDVMGQIHTA
                     MLANVTQRPVKLLFDRRESLLVHPKRHATQIRVKIGAKKNGRLVAAETELYGDTGAYA
                     SLGEKVMTRATTHSAGPYDIPHVRADCYAMYTNNPPAGAFRGFGVTQSAFAVESMMDK
                     LAEELNIDPVELRRMNALHVGSVTNTGQELKESVGLLECIDRVESEMLKVAGLPRGEL
                     FKPRVTPDTPHLVRSWGFASAYKNTGLGGGAPDISGADVELYADGTFQVRSSAAELGQ
                     GLVTVMRLVVSEELAVAPEKVRVLVMDTDLTPNGGPTTASRQTFVTGNASRYAAKTLR
                     EEITASMA"
BASE COUNT         2770 a         2968 c         2689 g         2246 t
ORIGIN      
        1 tagtgccttg gtgactttgt ggtctacaac actttactta tgcaagaggt caaataaagt
       61 ttttcgcacc agcaaaatga cagactttcc gaagttcgat cttcaacaca ttgcctaaac
      121 acaggcgggt tctccgatga gcccgcctgt gttattttgg attcttacga ccaaaacacg
      181 ccatcaggca tacaatgaaa tgaagcaggg taaaattcac gggtcaaagt ttttgaatat
      241 ctcatcacga ggtgaagcat gagcgatgtt tacgatctca agtcagtgaa acttccgtat
      301 ttggcgggcg gcgcgttgaa attgtttgcg gcactggtcg aagggccgct gggcggactg
      361 ctcacgccga gtctgtttga aagcgcagga gtcaactggc tgagaaagca aagcttcaac
      421 gatgcgccca cccaccatcc gattcacttt acgggggaac tgcaaaccga atcatctgct
      481 gtgcccgaaa acgagtggcc gcaaaatgca tccacaacaa agggctttca attcaaaagc
      541 gtacatgaac tcgcaaacgc ctaccgcagc ggaaaaacca cacctgaaga agttgccaga
      601 aaagttttag acgcgatcaa ggcaagcgac tcaaccgacc tgccgcttaa ggcattcatc
      661 gctgtgtaca acgaagatgt gatgcggcag gcacgccaat ccacacagcg catcaaagct
      721 ggcaaagcgc tcagcatttt cgacggcata cccgtagcca tcaaagatga aatggacatg
      781 gctccctacc ccaccacggt cggcacatcc tttttaggaa agaaccctgc ccaagaggat
      841 gcgaccgtcg tcgcccgttt gcgaaatgca ggcgcattac tcatcggcaa agccaacatg
      901 cacgagatcg gcatcaacgt cacaggcctg aatccgcatt tcggcacaac acgcaaccca
      961 tacagcccaa ggcattacac gggcggcagt tccagcggct ctgccacagc cgttgccgca
     1021 gggcttgtct cttttgccat cggcgcagat ggcggcggat cgatccgcat tcccgcttca
     1081 ttttgtggat tggtcggatt gaaggccacc tttggaagaa tcagcgaata cggcgcatac
     1141 ccgctggatt ggagcgtcgc tcacatcgga cctttggctg catccgcctc cgatgcagcg
     1201 cttgcctacg ccttgattgc tggccccgac cccaaagatc cgaactcatt gcatcaaccc
     1261 ctgccatccc tccaaggctg ggatgatctc aatctcaaag ggctcaagct tggcgtttat
     1321 tggccctggt tccgtcatgc ggacgcagaa acagtcgccg cctgcgaggc catgctcaag
     1381 gaatatgaaa aaatgggctg cgagatcgtg gaagtcacca tccccaacct cgaagcaaac
     1441 cgcatcgccc acagcgtaac catcacaagc gaaatggccc aagccatgaa tgagacctac
     1501 ggaaaacatc atcgcgacca tggactggat gtgcgcgtga acctggcact cgcccgccaa
     1561 ttttcagcca ccgactatct cacctcccag cgtatccgca cccgcatgat ccagcatttc
     1621 aacgacgcct tccaactcgc cgatgtcatc ctcacaccga ccacaggcat caccgcgcca
     1681 gagatcaaga aaggtgccct gcccgatggc gaatcggacc tgaccaacac catcgagatc
     1741 atgcgcttcg ccaccgccgc caacatgacc ggcctccccg ccatcacctt ccccgttggc
     1801 tacacccaaa aaggactgcc catcggtctg caggtcatgg gcaaggcatg ggatgagaaa
     1861 accttattgc gccttgcact ctccgccgaa caagttgtag aacgcaaagc cccacaggta
     1921 tatttcgaat tgctcaaata gccacccacg aaaagaacaa atgtgctaaa atcactccag
     1981 tttatccact agaaaaagga gaccatcatg gctgagctaa aaacaaaaaa gaccgaagca
     2041 agcgtggaag atttcctaaa cggcgtcaaa gacgaagcca caaaaaagga ctgttttgaa
     2101 attgtaaaat tgatgaaaca agccacaaag gcagacccca aaatgtgggg ctcaagcatt
     2161 gttggcttcg gcacccaaca cctcaaatac gccagcggcc gcgaactgga atggatgatc
     2221 cttggtttct caccccgcaa acaaaacctc accctctacc tgcccgggag ccttgagtca
     2281 tacgcaggca tgcttaagaa attgggcaag cacacaacag gtaaaggttg tttgtatatc
     2341 aaatcgctca aagatgtgga tacaaaagtg ttgaaagaac tcgtgcaaca ggcagtgaaa
     2401 accaccaaac aatgaaacca ctcaaagacc ccgaagaatt tgaaaaaaag gctctccaca
     2461 agtttgtgga tttttcaaat gcgcacgttt tggaaatcgg atgcggcgaa ggcaggttga
     2521 catggaaata cgccgccgcc tccaccctga ccgttggagt ggaccctgac caaaatgcat
     2581 tgcgcatcgc ccgcgcagac actccctaca gcctgcaaga gaaagtccgt ttttctcgag
     2641 caaccgcccg ccaactccct ttttcaaaag aaacctttga tattgccgtc ctagcctggt
     2701 cgctctgatg aatggaacac gagagcatgg tccatgctct ggatgaaatt cgaagaaccc
     2761 tcaaaccaaa cggaatcctg atcgacttgc gccccgtgga agaccactgg tcggttgaag
     2821 tggcctccca acaaaacatt ctgaccgcag gccagttgac cgacatgccc atcggcaaag
     2881 ccgacgatga agccgccttc cgcgccatgc gggaagtgga ggcacgcggc tggttcacgc
     2941 gggaacgcga agaggaattt tctttttttt actactggga tactccgtct gagatgaagg
     3001 agttcatgga aaaggaatgg aaagactttg aaaaaatgga agacaaagtt taccaaaccg
     3061 cgaaatcgct ctggtcaaca gccgatgcag atgcacagat gcgggtccgt gtgaaaatgt
     3121 tgatcacaaa atggagaaac acaacctgag cggatgcgcc tttacgaggt ttcgttcacc
     3181 aggtcatgaa tccattttcg tgacctgacg cgccagccac tgacaaaagc tttggcaagt
     3241 tcctccaacc caaccatcag ataaacataa tatacgggga gattgaacac aaacgcactg
     3301 gtatatgcca ccggcacgcc gatcagccag atggaacaga tctccatgat gagggcaaaa
     3361 cgggtatctc cacctgcacg taaagcgcca ataaatgtgg agaagttgaa catgcgaagc
     3421 cacaaagtgc aggccatcat caacatcagc atgcgcacat tgtgtacacc gctgggggaa
     3481 agatcgtata aattcgtaac aacatcacgc atcaaaacaa cgataatgcc cacaatccac
     3541 gcggcagaca ccccaaggat cacaatgcgc cgcacagttt cgtaggcttc ttcttttttg
     3601 cctgcaccga tacggtttcc aaccattacc gcacaggcat tgcccagacc cataaaaaca
     3661 acaaagccca gttcttcaat agtggcattg acattgatcg cggcgatcgc atctgtgccg
     3721 atgtgggcgt agatggcgtt gtaggtggtg attcccagcg accaaaacat ttcattggca
     3781 agcgcaggca gtgtggtttt gaggacacgg gtgaaaaatg aaaggtcgaa ggagaaaaat
     3841 gtcaacgggt tggcggcgag gggtgttttt tgggtgtaga ccatgaccaa tagcagaacc
     3901 agttccaggg tccagcctga agcggtgccg attgcggcac cttccactcc aagagcggga
     3961 acaggtccat agccaaagat cagcatataa ccaagaatcg tcttaaagat cagggctgtt
     4021 gttgtggcaa caaccgtgat cttgacgaat tgaatgctgc gcaaagtggc aatataggaa
     4081 gtggcaagtg caacgggaat ataggaaagc ccgacgatgc gaaggtaacg agtgccaata
     4141 tcgatcactt cagggtcggc cgtgtaaaag cccagaacgg tctgcggcat gagcgtggcc
     4201 gcaagagaaa atagaagcgc aatgagggtg gagacaagga tgctcatacc caacactttg
     4261 cggatcggct cgatctcctg cttgccccaa aactgggcgg tgaaaattgc catgccgctg
     4321 gtgaggccaa aaagcaggag aataaaaaca aagaacacct gattcgacaa tcccatggcg
     4381 gcaatggagg tttcgccaag ctgccccacc atgagtacat cgatcaaatt gagcgaagcg
     4441 ttgataagct gctgaaatga aatagggagc gcgatggtga gcatttcgcg caggaaggaa
     4501 cggtcacgga gaaaggagag ggacatgggg gaaaaggata aaggatgagg aataaaggat
     4561 gaaaaagtcg ttgaacgttt tgttttaagc gttcaacgac tgattgccat tcagcgattg
     4621 atatttactg gtcttccggt gcatcgcgga caatatacaa cggtcggcct ttggcttcat
     4681 cataaaggcg gccaatgtat tcgcccagaa tacccaacga gatcaactgc acaccgccaa
     4741 gaaaaagcac agaaaccagt gtagtggttt ggccttcaaa gaaatgcgaa cccgccagac
     4801 gcagaatggc cacaacagga atggcaagga tggaaatacc cgccgagatg aacccgaaat
     4861 acgtggccac ctgcaaaggg aaataggaaa agcccgtaat ggcgttcaac gcaagacgca
     4921 gcattttctt gaagggatat ttcgtcaccc ctgctacacg ggcggcgcgt ttgtaggtaa
     4981 ccccgatctg tttgaaaccc acccatgctg acatgccgcg cgggaaccga tggcgttcct
     5041 tcatctgctt gagcacattg accactttgc ggtccatgag gcggaaatcg cctgtatcca
     5101 cggggatctt cacatcggtg atgctataaa tgatgcggta gaacatggct gcggtgaatt
     5161 tcttgaacca ggtttcgcct tcgcgctcgc cgcgtacggc atataccacc tcataacctt
     5221 ccttccattt tttcgccaat tcaaggatca cttcgggcgg atcctgcaaa tcggcatcaa
     5281 taatgacgac cgcatcgccg cgggcataat cccagcctgc ggtgatggcc acctgatgac
     5341 caaaattacg cgcaaaaatc acggggcgaa ccgttttatc tttttcagcc agagcgcgaa
     5401 taccatcagt ggaaccgtcg gtggaaccat catctacaag gatgagttcc cacggctcgc
     5461 cagaagaatc catcacctct tttacgcgac gatacagttc gggaagatta tcaatttcgt
     5521 tatagatggg agcaatgatc gaataagtga ttttcataaa tcttctttcc gtttttcttt
     5581 tgtggatatt ttgatcggcc gccttgcttt tattttctca ggcggagcga caaagcgttt
     5641 ttcaccgtca tcttcaaagg gcggcaatcc cagtacgcgc cgccgcacat actccatgat
     5701 cacaaccaca gaagccagta cgggcaccac cagcaaagcg cccataattc cctccaggat
     5761 cgttgcgatc atgatggcga tgaagatcaa ggcttcattc atgtgcaaac tgcgccccat
     5821 gatgagcggg cgcacaaaga agttcttcaa attgatcagc accagatacg tggcaaacac
     5881 aatgatcgcc acaatcacat tcgagtacgg aatccagctt gacccttcca acagagccac
     5941 accgaccgcg aaagccgccg caagaaatgg ccccacatcg ggcacaagcg tgaacaaacc
     6001 agccattacg cccaacacca acgcaccagg aatgccaagg atcagccatg cgatcgtgaa
     6061 tacaattccc accaccagca tcagcacgat ctgcccacgc aggtatgcca tccacacatt
     6121 gcggaggcgt ttgtacaatt cgcgcatatc atggtggtac tcctcggggg cgaggtccac
     6181 cagccaatca cggatgctcg gccattgact cataaaaaga tgcacactga caatgatcac
     6241 caaaaaccac aacacattca ccgatgtggt ctccagcaaa gccagtgctt cttcgggcag
     6301 aggggtcagg acggtatctt gcaaatgtgc gaggctttca cccaattgtt cgaaatgaaa
     6361 agacattcca ccaaattgaa ttgggctgga aagcaccgca ctaaattcag ccgaaaggtc
     6421 gagtatgtcc tgggcaacga tcttgatctc gtcgaaaaag atcggcgtca acgtggcggg
     6481 cacgcccacc aaaaagatca gcgccgaaaa ataaacgata ttcaccgccg cagtccgcga
     6541 gagttttgtt ttttgcatca acaaaatcac agcagggctg atcaggtatg aaacaaaccc
     6601 agcgatcacc agcatcttca ccgcttcgcg cgcatagatc aacagggcaa caatggcaat
     6661 aaaaacaaca aagcccattg cataacggaa aggaagactc cactggctga ttttcttttt
     6721 cataataatt ttttcaccgc ttcgagggta ggttcaatga tcggcgcgga ttggacagcc
     6781 tgcatcgccg cgcggacatc cttgaggccg ctgccagtat tcatcaccaa aatgggatca
     6841 tcgcttccaa cgaccccgag gctggtcgct tttaccaaac cagcataagc cgtggccccg
     6901 gcaggttcgg caaagactcc cattttgccc agttccgcaa tggacttgat gatctcgtta
     6961 tctgccacag tgatataggt gccgtccgtc tctttggcgg cgcgaactgc acgcacccca
     7021 tcacgcggca ggtctacgga aatgctgtct gccaaagttg tggcagagac tggcgtaatc
     7081 gtttcggtat ttgcattgaa cgcattcgcg atcgccgccg acccttccgc ctgcacaccg
     7141 atgatgcgcg gcatgttggg aatccaaccc agggcgagca ggtctttgaa tcccttgtga
     7201 atgccagaga tgatgttgcc gtcacccact gaaacaaaaa tacaaagcgg cgggtgatta
     7261 tcgagcggac tgtctttctt gtgccagtcg cggtgcgctt ccatccacca ctcccaaatt
     7321 tcgaaggcag cggtcttttt cccttccact gtaaagggat tgtagcccgt gttgcggcaa
     7381 taccagccaa attcgtcggc ggctttcacg gtcaggtcga aagcgtcgtc gtatgttcca
     7441 tccaccaaaa tgacctttgc cccaaaaacc agcaattgcg ccacttttgc aggcggtgca
     7501 gacttgggag caaagataac tgctttttgg cccaccgccg ccgccatgca cgccagtgcc
     7561 gcgcctgcat tgcctgtgga tgcggtcaca accacttcgg ctttgatctc ctgtgcccgt
     7621 gccaccagaa tggcggaagc acggtccttg aaggaagccg acggattacg cgactcatct
     7681 ttcagccaga gatgtttcag gccgagtttt tctgccagcc gcggcagggc aaagacaggc
     7741 gtcccgcccg ctgcgtgcaa aggcgtggaa tcacctgcgg gttcagacac aggcaaaagc
     7801 ggtaaatact tccacagaga tggctctgtc cgcgaagtga tatcttcggg ctggtatttt
     7861 ttccgaatgg aatcaaagtc gatcacaaca tccagattcc cgccatcttt cggacaagtg
     7921 tatgtaacct gcccaggcag gtactctgtt ccacaaagtg agcatttgta gccagtgaaa
     7981 tttttcatag tggcatgatt ttacccgata gacaaggccc ggactagaaa aggactctca
     8041 ggctttcgcc atgagagtcc agttactaat caccgattac taatttactt ctcttcccgc
     8101 tcccacaact ctctggcgat cacttcctga tcgacaggca atttatcgaa cctgacgccc
     8161 accgcattgt agattgcatt tgtaatcgct ggcgtggtcg ggatgctgga aatttcaccg
     8221 acacccttcg ctccaaacgg acccgacgaa atgggatgct caacgataat ggcggtgatc
     8281 tccggggtga gcatgatgcc aggaacgcgg tagcgcgcca attgatctgt gaccacattg
     8341 ccgttttcga cgatgaattc ttcggtcagg cagttgccga ggcccatcat cacgccgcct
     8401 tcgacctgtc cctgcaagcc aagcggatta accgccatac ccacatcgtt ggcggagatg
     8461 accttcaaaa ccttcacctc gccagtcagt ttattcactt ccacttcggc ggcttgcaca
     8521 ccaaacgaga aggcgaagtg catgtcgccg cctgttccca atggctgggt cttcggcgct
     8581 tcatattcat agcggacttt gggctgctgt cccatgcttg tcatttcttt gtacacatcg
     8641 gcatacgaaa gcgaatgccc gttgacatgg acaatgccgt cttcaaaatg aatctgctcg
     8701 gggcgcacat cgaatttttc cgccatcgaa gcggtgatct cttcgcgcag ggtcttcgcg
     8761 gcgtagcgtg aggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgcca
     8881 tcgaagcggt gatctcttcg cgcagggtct tcgcggcgta gcgtgaggca ttgcccgtga
     8941 cgaaggtctg gcgcgaggca gtggtcgggc cgccgttcgg ggtcaggtcg gtatccatga
     9001 caagcacacg gactttttca ggcgcaaccg ccaattcttc agaaacgacc aagcgcatga
     9061 cggtgacgag tccctgcccc aactctgcgg cagaactgcg gacttggaag gtaccatcgg
     9121 cataaagttc cacatccgcg cctgagatgt caggcgcgcc accaccgagt cccgtattct
     9181 tgtacgcgga ggcaaagccc cacgaacgta ccaggtgcgg ggtgtcaggt gtcacgcgcg
     9241 gcttgaacaa ttccccgcgc ggcaaacctg caaccttcaa catttcactt tcgacccgat
     9301 caatacattc cagcaggcca actgactctt tcaattcctg tcccgtgttg gtgacgctgc
     9361 caacgtgcaa ggcgttcatg cggcgcagtt caacagggtc gatgttcaat tcttccgcga
     9421 gtttatccat catcgactcg accgcgaaag cagactgcgt gacgccaaat ccgcggaatg
     9481 cgcccgcggg cggattgttg gtgtacatgg cgtagcaatc ggcgcggaca tgcggaatgt
     9541 cgtacgggcc agccgagtgc gtggtggcac gggtcatcac tttttcgccg agcgaagcat
     9601 acgcgcccgt atcgccatag agttcagtct ccgccgcaac gaggcggcca tttttcttcg
     9661 cgccgatctt cacgcggatc tgcgtggcgt gccgcttcgg atgcacaagc aaactttcgc
     9721 ggcggtcaaa caataatttc acgggacgct gtgttacgtt ggcaagcatg gcggtgtgga
     9781 tctgccccat cacatcttcc ttgccgccga agccgccacc catcaactgc ccaacgatgc
     9841 gcacgcgctc ttcgggccag cccatcacgc gcgcaacctg tgtgcggtcc tgatacggaa
     9901 tttgcgaacc gacatagatt tccatgcgtc catcgggcag cggcacggca atgctgcact
     9961 caggctcaat aaaagcatgg tccgtggcag gtgtatggaa agtgtgctcg ataacaatat
    10021 cggcttcggc aaaacccgca tccatatcac ctttgcggac cttgatgtgc ttcagcaggt
    10081 tccccttctc gtggagttgc gggacgcctt cttcgcgagc catgaccgcg ttggtgatga
    10141 cgggctggag gtcgaactcc gcctcgatca acgccgaagc ctgctcggcc aactcaggcg
    10201 actcagcggc gacaatggca agtgcgtcgc ccacgtagcg cacgcgttcc ccgatgccca
    10261 ccatcacagg ccaatcgtaa atgacgaggc cgtgattctt ttcagcaggg acatcttcgg
    10321 cggtaagaac ggcgataaca cctttcaagg ctttcgcttt cgaggtatcc aattttttca
    10381 aaaagccatg cggaatcatg gcgcgtttgg ctttggcata caacatgccg tcgaacttca
    10441 agtcatcggt gtagatggct tcgcctgtga ccttctccac ggcttcggga cgcaggtgtt
    10501 tgtgtccgac ggttttatgc tcgtggacag agtctggaat atgcgtcggc ttctggacag
    10561 gctcgcctgt gcgcaatgcc tgcgccgcag caatgatcgc gttttcaatg gatggatagc
    10621 ctgcacaacg gcaaagggtg tctttcaaag caaaacggat gtcatcgttg ttcgggtcta
    10681 ggttgcgatt cagcagggca tacgcggtca tgatctgacc ggggatgcag aatccgcatt
    10741 ggacagcgcc atgctccaca aaggcttcct gca
//
LOCUS       DAOQLC010000135        10388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2481_length_10388_cov_25.309107, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000135 DAOQLC010000000
VERSION     DAOQLC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2481_length_10388_cov_25.309107"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PL000_17895"
     CDS             complement(<1..193)
                     /locus_tag="PL000_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90808.1"
                     /translation="MKKLLFTLLFLSLLAACIPATESPPQTQTTSAAATDSSFMQSVQ
                     PSSLPLPTETNKPKGHNQPV"
     gene            complement(264..2081)
                     /locus_tag="PL000_17900"
     CDS             complement(264..2081)
                     /locus_tag="PL000_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90809.1"
                     /translation="MYKKIFSLLLICSLLTTGFTFASTAPQPASAQAATATFEDVPTT
                     YWAYQWIEALAANSITGGCSTTPKLFCPDTLVTRAQMAVFLLRSKHGNAYTPPAATGT
                     VFTDVPAGYWAADWIEQLSAEGITSGCSTGLYCPETIVSRDQMAIFLLRSEYGSSYIP
                     PAATGTVFTDISADYWAASWIEQLVNEGITAGCSDTGYCPEQSVTRAQMAVFLVRAFN
                     LPPTPPAFNLDQTLSDGAQLNTIAFDGLAFITGSLGADSFFPPGKVADFWGFQYLRDN
                     DPSEMGHNTDFLTRASLNLLYVLTSDQKAALITLAKNQVASINEYGYKRFVLMKAFRR
                     LVEGDLPAGTTGLNPDAVKAYSAELYQLDGQISYERAQIMGGIISAFTTEQRAYLDAM
                     VGQGMTSWPVVNEPSELKDLTQDEKIAVMTYAGDMFSWYAGSFQADVYFCPERQGTYF
                     GSFYMKDAPAMGNPDFTIPSNLTAEMGDAFLKTLTADQAALINNLVTDQKPALYGIVS
                     SREEVSTMLRQFMAGGTPDQTAILRLMETYGELDGEIITSYIFAFNQINQTLTASQRA
                     QLLKLRVDWLQDLNQPTGAFLYSQAITMPEIPNTDFLFK"
     gene            complement(2124..3953)
                     /locus_tag="PL000_17905"
     CDS             complement(2124..3953)
                     /locus_tag="PL000_17905"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90810.1"
                     /translation="MYRKFLSILFLTVLLTGCMTPPSVTETILVPEVSETAVVTSPVL
                     QLLNTSFILTSTDVSNGGVLPVEFTCDGNGSTLPLAWSGAPSETQSYAVIMHHEAPDG
                     IHWYWVLYNIPADVTSLPKNVSGIGTLGNNINNGLVEYSPPCSKGPGDKIYTYTVYAL
                     AAEPQINVPAEQVNRDTLLAAIQNITLASAELNVVYAREGMTDPNLQGNQQEQGNNQQ
                     SNNQLTNIAPAYNIEQATSDKAQGMTISYDALAFMTGDLGSDSFFPPGKVADFWGFQY
                     LRDNDPSQMGHAGDFLTSAAMNTLNNLTANQRAQLVALAQSQVDDINTYGYKRFILMD
                     AFRRLAEGDLPAGTTGLNEEAVKAYSAELYQLDGQISYERAQLMGQILISLSAEQRAY
                     FDAMVGKGMLEWNDVQEPEDVRGLDRDTKVAVMTYAADLFSWYAGSVEADVYFCPERH
                     GTYFGSFYLKDIKAMSDPTYAIPTNLTGDLGETMLQKLTPDQAALITGLVDNQKSALQ
                     GMVDAREQISIELRKFKDGSAADQATIMSLMEQYGAYDGEIIYNMAVNFAAVKSSLTS
                     EQQAELDALRTELLGDQSHPTGAYLYSQAIPMPEIPNSDFLFK"
     gene            4146..4367
                     /locus_tag="PL000_17910"
     CDS             4146..4367
                     /locus_tag="PL000_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90811.1"
                     /translation="MGETSSTKTGTFEPVGPVPEHQRKGLARVLMAEGLKRLQKLGAV
                     MVYVSSYGKAAHATYESVGNHLELWEKSF"
     gene            4522..7236
                     /locus_tag="PL000_17915"
     CDS             4522..7236
                     /locus_tag="PL000_17915"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC90812.1"
                     /translation="MSTPVLATKLSIPAPRPKIVPRPHLTDQLNVGLHRKLTLVSAPA
                     GFGKTTLVAEWIASCERPVAWLSLDEADNDPIRFLTYLIAALQTIVSTIGADVLRILQ
                     SAQPQSSEAVLTILINEIAAISDDFILVLDDYHLIEEKTIGNALTFLLEHMPPQMHLV
                     IITREDPNLPLARLRVRDQLTGLRATDLRFTDSEAAEFLNQVMGLRLSAEDIAALEAR
                     TEGWIAGLQLAAISMQGHKDAGGFIKSFTGSNQFVLDYLIEEVLHQQPESIQAFLLRT
                     SILDRMCGSLCDAVLLDSSTSGQETLESLEHSNLFIVPLDNERHWYRYHHLFGSLLRQ
                     RLGQSWKPEEIARSHIRASEWFEKHEDPAQAFHHAIAAGDHSRAAALAEKYWQGMNEN
                     FQSAAWLGWVKQLPERLIRSRPVLCTQIAWVYMDLSEAEASESRLRDAEQCLENPTDE
                     MVVVAEEQFRSLPARIAIARTYNAQSVSDYPAALKYAKLAIKLAPEEDHFLRAQTNAI
                     LGGTYWANGDLDAACKSMSDWIDNAQKAGNYIFAIASASGKADILMEQGYLREALRTY
                     QQSLQLASEHETESQHIIAHHHLGLALLYHEMQNDESAAQHFQKSMELGQYSVLQDWA
                     YRKNLAQARLKESEGDLDSALGFLDDAKRFYIRSLIPNTRPIDAFKARIFLKQGRLSN
                     AQEWVRQHGLSVSDDPGYLREFELGTLARVLLSEYQTHRKERIINDALNLLKRLLKAA
                     EDQKRMGSILEILMVTALAYHALGSTSQAFVTLERALTLARPEGYVRIFVNEGDLMRA
                     LLTDFRASNEKQRRGNGSDLKDYVDKLLSAFTQPKDVQRSKLIEPLSKRELEILQLIA
                     QGLSNQEIGKRLFLALNTVKGHNRIIFDKLQVQNRTEAVARARELDLL"
     gene            7460..8749
                     /locus_tag="PL000_17920"
     CDS             7460..8749
                     /locus_tag="PL000_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNC90813.1"
                     /translation="MTNGQNLKTDPGQPMVYQIRIKGHLNDQWVDWFEGMNIILEGNG
                     NTLLTGPVIDQSALHGLLKKVRDLGMTLLSVNGVHLDQAEAPEASQNFLLSNQNNLGG
                     ILMKAIVYTKFGSPDVLKLQEVAKPAPKDNEVLIRIVATTVAVEDPGIRARPGLNGFL
                     KPKRSILGFYLAGEVESVGKDVKRFKKGDKVYGNTGLSLLGTYAEYICMPEDGALAMK
                     PANITDEEAAALPNGALTALPFLRDKGNIKSGQKVLIYGASGAVGTIAVQLAKYFGAD
                     VTGVCSTTNLELVKSLGADKVIDYTQEDFTKNGQTYDIIFDTVGKSAFSRCKDSLTEN
                     GIYLTTVPSLATFIQMLRTSMTGGKKVGIAATGLRPSNEKTKELIFLNGLIEAGKIKA
                     VIDRSYPLEQIAEAHKYVEAGHKKGNVVITLNHNAKN"
     gene            8801..9793
                     /locus_tag="PL000_17925"
     CDS             8801..9793
                     /locus_tag="PL000_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNC90814.1"
                     /translation="METKANTAKMKAVVNTQYGSPDVLQLKEVERPTPKDNEVLVKVH
                     AVSVNAADWHLLRADPFLVRLQFGLFKPRHTILGADIAGRIEAVGRDVKRFKVGDDVF
                     GDISGSGWGGFAEYACAREDTLVQKPANLSFEEAAAVPMAAVTALQGLRKGGIQPGKK
                     VAINGASGGVGTFALQIAKAFGAEVTAVCSTRNVELARSLGADHVIDYTQEDFTKNGK
                     QYDLILATNGYHPLADYQRALSPTGSYVMTGGTNAQMTEAMLYGPLKSRNGKQQMGNL
                     LAQPNQKDLETVAELLAGGKVKSIVARRYSLAETADAIRCVEAGHAQGKIVITT"
     gene            9863..>10388
                     /locus_tag="PL000_17930"
     CDS             9863..>10388
                     /locus_tag="PL000_17930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90815.1"
                     /translation="MSFATFWAEDTLTSIASLPGFTAGTAFDWDELAGINRIPQAEVM
                     DRVNHGHRPYVARMDGQPVAYGWLATSKISIGELGINVELPSGDRYLWDFATLPDWQG
                     RGFYPRLLQSILEQEIQNAKRFWIIHAPENLPSGAGMSKAGFEFVGQLSFTWDGKIGL
                     APYADSTERARIGAD"
BASE COUNT         2588 a         2426 c         2839 g         2535 t
ORIGIN      
        1 cgacaggttg gttatgccct ttgggcttat ttgtttcggt gggcaaaggc agggatgaag
       61 gctgaacgct ttgcatgaag gatgaatcgg ttgctgctgc agaagtagtt tgtgtctgtg
      121 gtggtgattc tgtggcgggt atacaggcgg cgaggagcga cagaaagaga agagtgaaaa
      181 gaagtttttt cataagcctt ttgatcacaa aagagacaaa gggtcactaa ggaaaaacct
      241 tagtgaccct ttatagttac gaattacttg aacaggaaat cagtattcgg aatttcaggc
      301 atggtgatgg cttgagagta caggaaggct cctgtgggct ggttcagatc ttgcagccaa
      361 tcgacgcgca gtttcaacaa ttgcgcgcgc tggctggcgg tgagggtttg gttgatctga
      421 ttgaaggcga agatgtagct ggtgataatc tcgccatcga gctcgccata ggtttccatc
      481 agcctgagga ttgcggtttg atcaggagtg ccgcctgcca tgaattgtcg cagcattgta
      541 gagacctcct ccctgctgct gacgattcca tacaaagccg gtttctgatc agtcacaagg
      601 ttgttgatca gtgccgcttg atctgctgtg agagtcttga gaaaggcgtc acccatttca
      661 gcggtgaggt tggatggaat ggtgaaatcg ggattgccca ttgcaggcgc atctttcata
      721 tagaaggaac cgaagtaggt gccctgccgt tcgggacaga agtagacatc ggcttgaaaa
      781 gaaccggcgt accaactgaa catgtcacca gcgtaggtca ttactgcgat cttttcatcc
      841 tgcgtaagat ctttgagttc tgaaggctcg ttcaccacag gccatgaggt catgccctgt
      901 ccgaccatcg catcaagata agcgcgttgc tcggttgtga acgcagagat gattccgccc
      961 atgatctgtg cgcgttcgta actaatctgg ccgtcgagtt ggtagagttc tgcagaatag
     1021 gctttgactg catctggatt taagccggtt gttccagcgg gcaggtctcc ttctacgagg
     1081 cggcggaatg ctttcatcag cacaaagcgt ttgtagccat attcattgat cgatgcaacc
     1141 tggtttttgg cgagtgtaat tagcgcggct ttctggtcag aagtgagtac gtagagcagg
     1201 ttcaatgaag cacgggtgag aaagtcggta ttgtgtccca tttcgctggg gtcattgtcg
     1261 cgcagatatt gaaagcccca gaaatcagcg actttgccgg gagggaagaa tgaatctgcg
     1321 ccaaggctgc cggtgataaa tgcaaggcca tcaaaggcga tggtgttcaa ttgagcgcca
     1381 tctgaaaggg tttgatcgag gttgaatgcg ggcggtgtag gtggcagatt gaatgcgcgg
     1441 acgagaaaga ccgccatctg tgcgcgtgta accgactgtt cagggcagta tcctgtgtca
     1501 ctgcacccgg cggtgatgcc ttcgttgacc aattgctcaa tccaacttgc ggcccaataa
     1561 tcggctgaga tatcggtgaa gacggtgccg gttgcggctg gcgggatgta gctgctgcca
     1621 tattcagatc ggagcaggaa gatcgccatc tgatcgcgtg agacgatggt ttccgggcaa
     1681 tataggccgg tggaacaacc tgaggtgatg ccttcggctg agagttgctc gatccaatcg
     1741 gctgcccaat agcctgccgg tacatcggtg aagacggtcc ctgtggcagc gggtggagtg
     1801 taggcattgc cgtgtttgga tcgcaacaga aagactgcca tttgtgcgcg ggtaacaagc
     1861 gtatcaggac agaataattt gggcgtggtg ctgcatccgc cggtgatgct gtttgcagcg
     1921 agcgcttcga tccattgata cgcccaatag gttgtgggga catcttcgaa tgtggctgtc
     1981 gctgcttgtg cagaggcagg ctgtggcgca gttgacgcaa aggtgaaacc ggttgtcagc
     2041 agggaacaaa tcaagagtag tgagaagatt tttttgtaca ttgtttcatc ctttcttttg
     2101 aaatggttcg tttcagtttt tgattacttg aagaggaaat cagaatttgg aatttcaggc
     2161 atggggattg cttgtgagta caggtaggcg cccgtgggat gagattggtc accgagcagt
     2221 tctgtgcgca gggcgtcgag ttctgcctgt tgttcggagg tgagcgagct cttgactgcg
     2281 gcgaagttca cggccatgtt gtagatgatc tcaccgtcgt acgcgccgta ttgttccatc
     2341 aggctcatga tggttgcctg atcagccgca ctgccgtctt tgaacttgcg aagttcaatg
     2401 gagatctgtt ctcgggcatc taccattcct tgcagggcag atttctggtt gtcaaccagg
     2461 cccgtgatca gcgcggcttg atcgggagtc agtttttgca gcatggtctc gccgaggtca
     2521 cctgtcaaat tagtggggat ggcataggtc gggtcgctca tggcttttat gtctttgagg
     2581 tagaacgagc cgaagtaggt gccgtggcgt tcggggcaga agtacacatc tgcttcgaca
     2641 gagccagcat accaactgaa cagatcggca gcgtatgtca tcacagcgac tttggtatcg
     2701 cggtcaagtc cacgcacatc ttcgggttcc tgaacatcgt tccactccaa catgcctttg
     2761 ccgaccatcg catcgaagta ggcgcgctgt tccgcgctaa ggctgatgag gatctgtccc
     2821 attaattggg cgcgttcgta gctgatctgt ccatcgagct gatacaactc cgcagagtag
     2881 gctttgaccg cctcttcatt gagtccagtc gtgccagcgg ggaggtcacc ctcggcgagg
     2941 cggcggaagg cgtccatgag gatgaagcgt ttgtatccgt aggtgttgat gtcatccacc
     3001 tggctttgag caagagcaac gagttgagcg cgttgattgg cagtgagatt attgagtgtg
     3061 ttcatcgcgg cactggtgag gaagtcgccc gcatgtccca tttgggaagg gtcattgtcg
     3121 cgcagatatt ggaagcccca gaagtccgca actttgccag gagggaagaa agagtctgag
     3181 ccgagatcgc ccgtcatgaa tgccagggca tcgtaagaga tggtcatgcc ctgagcttta
     3241 tctgaggtgg cttgctcgat attgtaggcg ggtgcaatat tggtgagttg attgttgctt
     3301 tgttggttgt taccctgttc ctgttgattc ccctgtaaat tcgggtccgt cataccttcg
     3361 cgggcataca ctacattcag ttcagcgctg gcaagggtga tattttgtat ggctgcgagc
     3421 aaggtgtcac gattcacctg ttcagcagga acgttgatct gtggttcggc ggcaagcgcg
     3481 tacacggtgt atgtgtatat cttgtcaccg ggtccctttg agcagggcgg ggaatactcg
     3541 acaagcccat tgttgatgtt gttgccaagt gtgccgatac cgcttacatt cttgggtagg
     3601 ctggtcacat cggcggggat gttatacaac acccaatacc aatgaatacc atcgggtgct
     3661 tcatgatgca tgatgactgc gtagctttgt gtctcgctgg gggctccgct ccaggcgagc
     3721 gggagagtcg aaccgttgcc gtcacaggtg aattcgacag gcagtacgcc gccgttcgaa
     3781 acatctgtac tggtgaggat gaagcttgtg ttgagcaact gcaaaacagg agacgtgaca
     3841 acagcagttt cactaacttc aggaacaaga atggtttctg taaccgaggg tggtgtcatg
     3901 catcctgtta gcaaaacggt gagaaagagg atggagagaa atttgcggta catatcattt
     3961 tttcctttca ctcctacgat agcaggaaga tgtaaggaaa ttgtttagaa gggtttagga
     4021 ttttgtttac gccatccttt caagggtttt tgagagactg taaaataaat acaagtcatc
     4081 tcaaaaatga ttaaaccgtc caaagccgct ggatttccct tataaatttg ggagttttac
     4141 tcaacatggg tgagacaagt tccactaaaa ctggaacctt cgagcccgtt ggcccggtcc
     4201 ctgaacacca gcgcaaagga cttgcgagag tattaatggc cgaagggttg aaacgactgc
     4261 aaaaactcgg tgcggtcatg gtatatgtca gctcatatgg caaagccgca catgcaactt
     4321 atgagtcagt tgggaatcat ttggagttgt gggagaagag tttttaggaa ttgacttctg
     4381 ggtttgtttg ttggcatctc tttcgatttt atgaagaaat aaaacaccca atcacattcc
     4441 gcccgtggaa gattacccaa acggtgatct caatgatctt ggaaggtgtt aatttttgaa
     4501 tattggtttt ataatgaatc catgtccaca ccagttttgg ccacgaaact ttccatccct
     4561 gcaccgcggc ctaaaattgt ccctcgtccc cacctcactg atcaactgaa tgtaggtctg
     4621 caccgtaaat tgacccttgt ttctgctcct gcaggctttg gaaaaaccac cctggtggcg
     4681 gaatggatcg cgagttgtga aagacctgtc gcttggttgt cgttggatga agcggataat
     4741 gaccccatac gatttctgac gtatctgatc gctgctctgc agacgattgt ttcaaccatt
     4801 ggggcagatg tgctgcgtat acttcagtct gctcaaccac agtcatccga agcggtgctg
     4861 actattttga tcaatgaaat tgccgccatc tcggatgatt ttatccttgt cctggatgac
     4921 taccatctga ttgaagaaaa aacgattggc aatgcgctaa ccttcttgct ggagcacatg
     4981 ccgccacaaa tgcatttggt gatcatcact cgtgaggatc caaatctgcc tttggcccgg
     5041 ttgcgtgttc gcgatcaatt gaccgggctg cgagccacag acctgcgttt tacagactct
     5101 gaagctgccg aattcctcaa tcaggtgatg ggattacgcc tttcggcaga agacatcgct
     5161 gcgctggagg cccgaacgga aggctggatt gcgggattgc aattggcggc gatctcaatg
     5221 caagggcata aagatgctgg gggattcatc aagtctttta ccggcagcaa ccaatttgtc
     5281 ctggactatc tgatcgaaga agttttgcat caacagcctg aaagcattca ggcttttttg
     5341 ctgcgcacat ccattctcga ccgcatgtgc ggttcccttt gcgatgccgt cttactggat
     5401 tcttcaacat ctgggcagga gacgttggaa agcctcgaac acagcaacct gttcattgtt
     5461 ccgctggata acgagagaca ttggtatcgt tatcatcacc tctttggaag tctattacgc
     5521 cagcggttgg gacaaagctg gaagcccgaa gaaattgccc gatctcatat tcgcgccagt
     5581 gagtggtttg agaagcacga agacccggct caggcattcc accacgccat cgctgccggg
     5641 gatcacagca gggcggcggc gttggctgaa aaatattggc agggtatgaa tgaaaacttt
     5701 cagtcggctg cctggctggg ttgggtgaag cagctaccgg agcgattgat ccgttcgcgt
     5761 cccgtacttt gcacacagat cgcctgggtg tatatggatc ttagtgaggc ggaagccagt
     5821 gagtcccgcc tcagagatgc tgaacagtgt ctggaaaatc caacggatga aatggttgtt
     5881 gtggcggaag agcaatttcg ttccctgcct gcgcggatcg cgatcgcccg cacgtacaat
     5941 gcccaatctg taagcgatta tcctgccgcg ttgaagtatg caaaactggc gatcaaactc
     6001 gctcctgaag aagatcattt tttgcgtgcc cagaccaatg cgattttggg cggcacgtat
     6061 tgggcaaatg gcgatctgga tgccgcctgc aaatccatga gcgattggat cgataatgcg
     6121 cagaaagccg gtaactatat ttttgccatt gccagcgctt ctggaaaagc ggatattctg
     6181 atggagcaag gatatctccg tgaagcatta agaacctatc aacaatccct gcagcttgct
     6241 tcagagcatg aaaccgagtc gcaacacatc attgcgcatc atcatttggg gttggcactg
     6301 ctttaccatg aaatgcaaaa cgatgaatct gccgcgcagc attttcagaa gagcatggag
     6361 ctcggtcagt attccgtttt gcaagattgg gcatatcgta aaaaccttgc tcaagcccgg
     6421 ctgaaagagt ctgagggaga tctggactct gcactcggct ttttggatga tgccaaacgc
     6481 ttttacatca ggtctctcat acccaacaca cgtcccattg atgcattcaa ggcgaggatt
     6541 tttctcaaac aaggacgatt gtccaatgcg caggaatggg tgcgccagca tgggctttcg
     6601 gtgagcgatg atcctggcta tttgcgcgag ttcgagcttg gtaccctggc gcgggtgctt
     6661 ctgtctgagt atcaaaccca tcgaaaagaa cgcattatta atgatgcatt gaacctgttg
     6721 aagcggctgt tgaaggcggc agaagaccaa aaaaggatgg ggagcattct cgaaattttg
     6781 atggtaacgg ctcttgccta ccatgcactt ggcagcacat ctcaggcatt cgttacgctg
     6841 gaacgcgctc tgaccctggc aagaccggaa ggctatgtcc gcatctttgt aaatgaaggc
     6901 gatctcatgc gggcattgct tacggatttt agagcctcca atgaaaagca acggcgcggc
     6961 aatggcagcg acttgaaaga ctatgtagat aaactcctct ctgccttcac acaacccaaa
     7021 gatgtacaac gatcaaaact gatcgagccg cttagcaagc gtgagctgga aattttgcaa
     7081 ctcattgccc agggtctctc aaatcaagag atcggcaagc ggcttttcct tgccttgaac
     7141 accgtaaaag ggcacaatcg aattatcttt gacaagttac aggtccaaaa tcgcaccgaa
     7201 gccgtggcgc gtgcccgtga attggatctg ttatagctgc acggttactt gatcgataaa
     7261 tctgatttta ggtgctgagc cacgcacgaa gggggatttt gttcgtggct ttaatacccc
     7321 ttccgtcaca ccccatccaa aaacaacacc cccaataaca cttaagtgtc tattcgttaa
     7381 cacctccttg taggtaaact aaccctacac tgaataagtg gtgcggactg aaacaaacgg
     7441 ccacaaaagg aacaacggaa tgacgaatgg acaaaacctg aaaaccgacc caggacagcc
     7501 aatggtttat caaatcagga ttaagggtca cctgaacgat caatgggtag attggtttga
     7561 gggcatgaac atcatccttg aaggcaacgg caacacgctg ttaacgggcc cggtgatcga
     7621 tcagtccgca ttacatggat tgctaaagaa agtacgcgat ctgggcatga ccttgctatc
     7681 ggtaaacggc gtccatctcg atcaggcgga agcgcctgag gccagccaaa atttcttatt
     7741 gtcaaatcaa aataacctcg gaggaatact tatgaaagca attgtgtaca ccaaattcgg
     7801 atcaccagac gttcttaaat tacaggaggt agccaagcct gcccccaagg acaatgaagt
     7861 actgataaga atcgttgcaa caaccgttgc agtagaagac ccaggtatac gcgcccgtcc
     7921 aggtctgaac gggtttttga aacccaagag atccatcctg ggattttatc tggctggcga
     7981 agtggaatcc gtcggaaaag acgtaaaacg attcaagaag ggcgacaaag tgtatggaaa
     8041 taccgggctg agcctattgg gtacctatgc cgaatacatt tgcatgcctg aagatggggc
     8101 gcttgcaatg aaaccagcca acatcaccga tgaggaagcc gctgcccttc ccaacggggc
     8161 attaaccgcc ctgcccttcc tgagagacaa gggaaatatt aagagcggac aaaaagttct
     8221 tatctatgga gcatccgggg cggtgggaac cattgccgta caactcgcca aatactttgg
     8281 cgcggatgtc actggggtat gcagcaccac gaatctggaa ttggtcaaat cgttgggagc
     8341 tgacaaggtc attgattaca cccaggaaga ttttacaaag aatggtcaga cgtacgacat
     8401 catttttgac acagtgggca agagtgcgtt ttcacgctgt aaggactcgc tgaccgaaaa
     8461 tgggatctat ctaaccacag ttccgtcact ggcaaccttc atccaaatgc tgcgtacatc
     8521 catgacaggc ggaaagaagg tggggatcgc ggcgaccggg ttgagacctt ccaacgaaaa
     8581 gacgaaggaa ttgattttcc tcaacggact gatcgaggca ggcaaaataa aagcggtcat
     8641 tgaccgaagc tatccgctgg agcagatcgc cgaagctcac aagtatgttg aggcagggca
     8701 taaaaaggga aatgtcgtca tcaccctgaa ccataatgcc aaaaactaaa ttacagagaa
     8761 taagaagata aagacgaatt cgatcacagg agcaaatatc atggaaacaa aagcaaacac
     8821 agccaaaatg aaagcggtcg tgaacaccca atacggatca cccgacgttc tacaactcaa
     8881 agaggtcgaa cgccccacgc ccaaggacaa tgaagtgttg gtaaaggtcc atgctgtgtc
     8941 tgtcaacgct gccgactggc atctcttgcg cgccgaccca tttcttgtcc gtttgcagtt
     9001 tgggcttttc aaacccaggc acacgatcct tggcgctgac attgccgggc gcatcgaagc
     9061 cgtaggcagg gatgtcaaac gcttcaaggt cggtgatgat gtatttggcg atatctctgg
     9121 ttctggctgg ggtggattcg cagagtacgc ttgtgcccgt gaagatactc tcgtgcagaa
     9181 acctgccaat ctctcgtttg aagaagcagc ggctgtgccg atggcggcgg tcacggcatt
     9241 gcagggactg cgaaagggcg gcatccagcc tgggaaaaaa gtggcaatca acggggcgtc
     9301 cggaggcgtt ggcacattcg ctttgcagat cgccaaagcc ttcggggcag aggtcaccgc
     9361 agtgtgcagt acccgcaatg tcgaactggc tcgttcgctt ggtgcagacc atgtcattga
     9421 ttacacccag gaagacttca caaaaaatgg aaagcaatac gacctgatcc ttgccacgaa
     9481 tggctatcat ccgttggctg attaccaacg cgccttatcc ccaactggca gctatgtgat
     9541 gacgggcggc accaatgcgc agatgacaga agccatgctc tatggaccgt tgaaatcaag
     9601 aaatggaaaa caacaaatgg gcaacctgtt ggcgcagccg aaccaaaaag atttggaaac
     9661 tgtcgctgaa ctgctggcgg gcggcaaagt gaaatctatc gttgccaggc ggtattcgct
     9721 ggcggaaact gccgatgcca tccgctgcgt ggaagcgggc cacgctcagg gcaagatcgt
     9781 gatcacaacg tagatctgat tttcattcca actctgaaac gtgttcttga atttattggt
     9841 agcactttta cgaggagaca aaatgtcatt cgccaccttt tgggctgaag ataccctgac
     9901 ctcaatcgca tcattacctg gatttacagc tggtactgcc ttcgattggg atgaactcgc
     9961 cgggatcaat cgcatccctc aggcagaagt catggaccgt gtcaatcatg ggcatcgccc
    10021 atacgttgcg cggatggatg gtcaaccggt tgcatatggc tggcttgcca caagcaagat
    10081 atccatcggc gaactcggaa ttaacgttga acttccatca ggtgatcgct atctctggga
    10141 tttcgccact ctcccagatt ggcagggacg cgggttttat ccgcgtttgt tacagtccat
    10201 cctcgaacag gagatccaaa atgccaaacg cttctggatc atccatgcac cggaaaacct
    10261 cccttccggt gcgggtatga gcaaggcagg atttgaattt gtcgggcagc tctctttcac
    10321 atgggatgga aaaataggcc ttgcccccta tgcagattca accgaacgcg cccgcatcgg
    10381 tgcggacc
//
LOCUS       DAOQLC010000136        10371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2487_length_10371_cov_22.001551, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000136 DAOQLC010000000
VERSION     DAOQLC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_10371_cov_22.001551"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PL000_17935"
     CDS             <1..728
                     /locus_tag="PL000_17935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC90816.1"
                     /translation="PGDLGFDVMHFNLHKTFSVPHGGGGPGSGPVGVSQRLVDYLPSP
                     LVGIIEEGDDDLEPLYGFVTPKHSIGRMKAFHGHFGGGLVRAYTYIAMHGPDGLKDVS
                     QYAVLNANYLQARLRDTYKIPYDRICMHEFVMEGQFEGSDIRALDISKRLMDYKFHPP
                     TNYFPLIVHEALMIEPTETENKDTLDRFADALIAIAEEAKSQPDLLHHAPHNTPFGRM
                     DEVKAARELVLCCWLPENYETLK"
     gene            868..1671
                     /locus_tag="PL000_17940"
     CDS             868..1671
                     /locus_tag="PL000_17940"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC90817.1"
                     /translation="MLNKLLFHRFPKAKSWLTRIQYELISTLDRDKNAIFMNFGYAPH
                     SHAYEPVALPKEEEIHRYPLQLYHHVARHIDWKNADALEVSSGRGGGAHFIMRHFKPR
                     SYTGVDFSTRAVDFCRRQYKLDGLKFHHGNAEALTFPDNSFDVVVNVEASLYYPNISK
                     FFEHVRRVLKPNGYFLYTDLRYEEKVAIWHAQLKNMGLKLIKEEDITDNVLKAMELDR
                     ARRVALVDRYVPAILRKQFYHFAGVSPDESETLPHLENRRYWYFVLQKA"
     gene            1771..2646
                     /locus_tag="PL000_17945"
     CDS             1771..2646
                     /locus_tag="PL000_17945"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNC90818.1"
                     /translation="MQPLSEDIRQKIILFQQTEITEYHIYKRLAKRIKSEGNAKIFDQ
                     IAEDELRHYNGWKEYTNEEVQPRWFFVWFYYLVSILFGFTFGVKLMEMGEEKAQANYE
                     AVSKVIPEATKYQDEEDKHEHQLIEMLDEERLQYAGSVVLGLNDALVELTGALAGLTL
                     ALQNVKLIALSGLITGIAASLSMAASEYLSTRSEETNKHPVRAAVYTGIAYIITVTLL
                     ILPYLLFENYYLDLVMALTTAVVIIAVFNYYISVAKGESFRERFVEMAGLSLSVAAFS
                     FVIGYFIRQWLGIEV"
     gene            2677..3843
                     /locus_tag="PL000_17950"
     CDS             2677..3843
                     /locus_tag="PL000_17950"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HNC90819.1"
                     /translation="MKILSVDIGTGTQDIFLYDSNLDIENGFKLVLPSPTMMVHRRLK
                     QALHTRTPILLSGYQMGGGPSAWAIEEMARAGIPVYMTPDAATTLNDELDKVEKLGIK
                     IVSEGEAKGLKGKVESLELRDFDFGLISKTFADYGVSLSDLSAIAVAVFDHGNAPAGV
                     SDRQFRFDYLNERIRAKNALSSFAFLSSDIPRIMTRLQSVADSAHGLPCPLVVMDTAP
                     AAVLGADFDPAVAQRKSKIVCNVGNFHTLAFRLGEMGIEGVFEHHTGEIDLPKLESLL
                     RALADGSLKHEDVFNDMGHGALMYSQSRFEFGKDDFDVVVTGPRRSLFHPSSGIPHPA
                     EHLRPYFAVPFGDMMIAGCFGLLAATAEILPELAEPVFGSLRSGRGRGVAPWDG"
     gene            complement(3891..5708)
                     /locus_tag="PL000_17955"
     CDS             complement(3891..5708)
                     /locus_tag="PL000_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNC90820.1"
                     /translation="MKKHLFLLLILPSLACSLLTGGGGNADPANPPSATQEETVDQQP
                     NIPAATPTPFDPLTGSGNSTRTVLTFDELMSGESSSPVDDSAFAIPADAAPPTFTFQG
                     KLELTDEAQAGGFQSLRDDFGYSADPAWMHLPEMNFEFVQSGSHLIPVDQSLVYSNQP
                     TWNYMVAPGRAWNESGDQGFSRASFPFALIERNQNCVHNGVMTFLFNDTTISNVRYQV
                     TQETCMYFKFDMWGQLKAAYTPSQVANAEAVTAAHFDWLKNQLPTKPMSALASDYPNA
                     KVNETFMSSGTTPEHTTVFGVFYNGVNYISNCNTRHGQYAYCSEMRLPSYSTAKSVLA
                     GMAYMRLGQLYDENVGDILVKEYLPEATNWDSITLEHTLDMATGRYEAPTYMTDEDFS
                     AISTNFLVAEKYDDKLKYALSFPASGKPGEQWVYQSTATFVATQTMYAYLRQQAGGDA
                     DLFDMLVQDIFIPLHLSTGTLSSLRTDNSPTGRAFGSHGMFYTQDDIAKIAAFLNSQS
                     GQINGEQILHPDLLAATMQRDSSDHGLSTGSELYNNSFWAKEYRSPCQFYVPFMWGYG
                     GISVAMLPNGATYYAFSDNGEFTFDGAVNELSKLSSVCP"
     gene            5841..6410
                     /locus_tag="PL000_17960"
     CDS             5841..6410
                     /locus_tag="PL000_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90821.1"
                     /translation="MNILFFVLGVAATVVLFAFFYLAAIRPSTERKWMQIPLPDEALV
                     NVYLSDDGDVLADGENGGLYEFYSDPDPVWKKVSEPEAQHPSVTCGPIMEDLYESLPP
                     PGEVSMMVSENCPFAEQANYLEVALLKNGETWFLKSSTNSYVGIGLFVLLPVGVLINI
                     VLYGIWLFFLAIDLILTQRHKRTQTASAP"
     gene            6513..6839
                     /locus_tag="PL000_17965"
     CDS             6513..6839
                     /locus_tag="PL000_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90822.1"
                     /translation="MKTLRMINGIFLGLSLCFALAIPILGIGSAAINWNGICYGFTDG
                     QVPCSWWEYTQNEMFWASFIFLPLLVMTLLMWGLMNLIRWVRRGAGTINSPRRFHDKE
                     NSHVQK"
     gene            6826..7305
                     /locus_tag="PL000_17970"
     CDS             6826..7305
                     /locus_tag="PL000_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90823.1"
                     /translation="MFKNKAIFLFLALCLTACTSQPALSAEGENWVAFSAEKAQADQM
                     LDWLFTTDAEYWSPTEADIHALESGLPAYLQENKSAFYMTETLIWERLDEYNRQYVGI
                     VLDGRKVIYANYLCQRGEGSGWKEQFAFVADGGACYFQFKFDTSTGGFFDLQVNGEA"
     gene            7375..>7702
                     /locus_tag="PL000_17975"
     CDS             7375..>7702
                     /locus_tag="PL000_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90824.1"
                     /translation="MRLYYWHYGVIVSSLATAVFHIMCFPYFGWFDPIVLNGFGSLAL
                     LAAYFLPIPFLQQNRRVTYWAIVAYTLLTIILWIILGDKTFQFATTAAIGYYAKVAEI
                     FLMVFMG"
     assembly_gap    7703..7802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8199..9455
                     /locus_tag="PL000_17980"
     CDS             8199..9455
                     /locus_tag="PL000_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HNC90825.1"
                     /translation="MNQSFHTENRRKLSAHMADGEVMLFFSGESVRKTADENFPFFTN
                     RNFLYLTGVKQEGSALLLEKKNGIVQERLFAAKPDFAQEIWHGRRFTEIELNQLSGVE
                     RIEDIANLNRALNELLSFQHITALWLCFDALASERSFDIEREFAKGIQGRHPHVVIRN
                     SYPLLAKMRKIKAPEEIEAIRKAMSITEAGIRRLMRISKPGVMEYELEAEFNSELASH
                     GQRRTAFPSILAGGERIFYLHYSNPMAAIADGDLILSDVGAAYDEYCTDISRVFPANG
                     RFSERQAQIYEVALAANRAIMAEVRPGVSFQVLNPICRQASFDGLKALGLLNDIADIG
                     KYVWHGAVHHVGLDTHDVGGYDEPIARDMIFTVDAGIYVREWGIGLRIEDNVLVTADG
                     CENLSAAIPTTLEEIEGFMAGAKAKN"
     gene            9452..>10371
                     /locus_tag="PL000_17985"
     CDS             9452..>10371
                     /locus_tag="PL000_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90826.1"
                     /translation="MITSTRKSDFVQVNGIRLHYLDWGGNGPTLIFLTGMGSSAYIYG
                     GFAPLFTDQFRVLALTRRGHGDSDYPETGYDADTLVEDIRQFMDALQIEKAAFAGHSI
                     AGVELTHFAATHPERVEKLIYLDALDDRRKERVVMEQHPLRNVQIKREESIPPRTLEE
                     YIANVKKEVPEFARIWSAVWDEEFAHVVVVNEEGIVVDKMPASIEKMMVENLIKDYAP
                     KVVKAQIPILSFYGKRKRKLTDDYTEEQKAAYEKFHRDVIEPFFKYLVDEFQGRFPQA
                     QIVVIPDGHHYCFMAQEELVHAEMRKFLLA"
BASE COUNT         2460 a         2443 c         2799 g         2569 t
ORIGIN      
        1 gtcccggtga cctcggcttc gatgtgatgc atttcaacct gcacaagacc ttctcggtgc
       61 ctcacggcgg cggcggacca ggctcaggcc cggtcggggt tagccagcgc ctggttgatt
      121 atctgccttc cccgctcgtc ggaatcatcg aagaaggcga tgacgacctt gaaccgctgt
      181 acggctttgt cacacccaaa cacagcatcg gacgcatgaa ggctttccac ggtcatttcg
      241 gcggtggact tgtccgcgct tacacctata ttgccatgca cggccccgat gggttgaagg
      301 atgtttcgca atacgcggtg ctcaatgcca attacctgca agcccgcctg cgcgatacct
      361 acaagattcc gtacgatcgg atctgtatgc atgaattcgt catggaagga cagttcgaag
      421 gcagtgatat ccgcgcgttg gacatctcga aacgcctgat ggattacaaa ttccacccgc
      481 cgacgaatta cttcccgctc atcgtccatg aagcgttgat gatcgagccg accgaaaccg
      541 aaaacaaaga caccctcgat cgttttgccg atgcgctcat cgccatcgca gaagaggcca
      601 aatcccagcc cgatctgctc catcacgctc cgcacaatac cccgttcgga agaatggacg
      661 aggtcaaagc ggctcgcgaa ttggtcctct gctgttggct gcctgagaat tacgaaacgt
      721 tgaaataaga atccccctcc ctgggctgct ctttaaaacg ggcaccacct gcacgaaatt
      781 acagggttat aatacgcggc aaccccgcac aaaagaacat ccagcatagc ctgctggatg
      841 ttctttataa ccacccaagg aggagcaatg ctgaataaac tgttatttca tcgttttcca
      901 aaagccaaga gctggctcac gcgcatacag tacgaactga tctccaccct ggatagggac
      961 aagaacgcca ttttcatgaa ctttggctat gctccccatt cacatgcgta tgaaccggtt
     1021 gcgcttccca aggaagagga aattcaccga tacccgctgc aactctatca tcatgtggca
     1081 aggcatatcg actggaaaaa tgccgatgcg ttggaggtca gctccgggcg gggcggaggc
     1141 gcgcacttta tcatgcgcca cttcaagccg cgctcctaca ccggggtgga tttttccaca
     1201 cgtgctgttg acttctgccg aagacaatat aagttggatg gactcaaatt ccaccacgga
     1261 aatgccgaag cgttgacctt tccggataac tccttcgatg ttgtcgtcaa tgttgaagct
     1321 tcgttgtatt atcccaatat ctcaaaattt tttgaacatg tgcggcgggt gttgaaacca
     1381 aacggatatt ttctctatac cgacctgcgt tatgaagaaa aggtcgcgat ctggcacgcg
     1441 cagctgaaaa acatgggctt gaaactcatc aaggaagaag atatcaccga caacgtcctg
     1501 aaagccatgg agttggaccg tgcgcggcgg gtcgccctgg tggatcgata tgtccctgcc
     1561 attttgagaa agcagttcta tcattttgca ggggtgtcac cggatgagtc ggaaaccctg
     1621 cctcatcttg aaaaccgacg gtattggtat tttgtcctgc aaaaggcata acgaaattca
     1681 caaacaaaag gatgcgccgt ttgacgcatc ctttttgatt tcttaaatcg cttacaattg
     1741 aaaacagaat tttccacaag gagtattttt atgcagccat taagcgaaga cattcgccaa
     1801 aagatcattc tgtttcagca gaccgagatc acggaatatc atatttacaa acgacttgcc
     1861 aaacgcatca aatccgaggg gaatgcaaag atctttgacc agatcgccga agacgaactg
     1921 cgtcattaca acggctggaa ggaatatacg aacgaagaag ttcaaccgcg ctggttcttc
     1981 gtctggtttt attatcttgt cagtattctg ttcggtttca ccttcggcgt aaaactcatg
     2041 gaaatgggcg aggaaaaggc gcaggccaac tatgaagctg tttccaaggt tattcccgaa
     2101 gcaaccaaat atcaggatga agaagacaag cacgaacatc aactcattga aatgctcgat
     2161 gaagaacgcc tgcaatatgc aggctcggtg gtcctcggcc tgaatgatgc gcttgtggag
     2221 ttgaccggcg ccctggccgg gctcacgctt gccctgcaaa atgtgaagtt gatcgccctt
     2281 tcaggcctca tcacaggcat tgccgcttcg ctttcaatgg cagccagtga atatctatcc
     2341 acccgttcag aagagacaaa caagcacccc gtccgtgctg cggtttatac gggcattgcc
     2401 tacatcatca ccgtgaccct gctcattttg ccgtatctcc ttttcgaaaa ctattacctc
     2461 gatctggtca tggcgcttac caccgcggtg gtcatcattg ccgtgttcaa ctattacatc
     2521 tcggttgcca aaggcgaatc gttccgtgag cgctttgtgg agatggctgg gcttagttta
     2581 agtgtggcgg ccttctcgtt tgtcattggc tatttcattc gtcaatggct tgggattgag
     2641 gtgtaggaca aacttaagtt tgtcctacga aatattatga aaattttatc agtagacatc
     2701 ggcacaggca cgcaggatat ttttctttac gattcgaatc tcgatatcga gaacggattc
     2761 aaactcgttt tgccatctcc gaccatgatg gttcaccgcc gtctcaaaca ggcgcttcac
     2821 acgcggaccc cgatcctgct cagcggatat caaatgggtg gcggcccctc ggcatgggcc
     2881 atcgaagaaa tggcgcgtgc gggcatcccc gtgtacatga cgcccgatgc tgcaacgaca
     2941 ttgaatgacg agttggacaa ggtggagaaa ttggggatca agatcgtttc ggagggtgag
     3001 gcgaaaggtt taaaggggaa ggttgaaagt ttggagttga gagattttga ttttgggttg
     3061 atttcaaaaa catttgccga ttacggtgtt tcactgagtg atctatcggc aattgctgtg
     3121 gctgttttcg atcatggcaa tgcgcctgcg ggagtctcgg acaggcagtt caggttcgat
     3181 tatctcaatg agcggatccg cgcaaagaac gccctctcct cttttgcctt tctttcttcc
     3241 gatattccca ggatcatgac ccgcctgcaa tcggtggcgg attcggcgca cgggctgccc
     3301 tgtccgctgg tggtgatgga tacagccccc gccgccgtgc tgggcgcgga ttttgaccct
     3361 gccgtggcgc agcgcaaaag caagatcgtt tgcaatgtgg ggaatttcca cacgctggcg
     3421 ttccgtttgg gtgaaatggg aatcgagggc gtttttgagc atcacacagg tgaaattgac
     3481 ctgccgaagc tcgagtcact tttgcgtgct ttggccgatg gctcgctcaa gcatgaagat
     3541 gtgttcaatg acatgggtca cggcgcgttg atgtattcgc agagtcggtt tgaatttggg
     3601 aaggatgatt tcgatgtagt ggtgacaggg ccaaggcgtt ccctttttca tccttcatcg
     3661 ggcatccctc atcctgcgga acatctgcgc ccgtattttg ctgtgccgtt tggcgatatg
     3721 atgatcgcag ggtgttttgg tctgctggct gctacagcgg agattctgcc tgaattggct
     3781 gaacccgttt tcgggtcgtt gcgaagcggg cgcgggcggg gagtggctcc gtgggacgga
     3841 taatgccgaa gggttgagga tgcctgcggg gcagacatcc tcaaaaagaa ttaggggcag
     3901 acagaagaga gtttggaaag ttcgttgacc gcgccatcga aggtgaattc gccgttatcg
     3961 ctgaaggcgt agtaggtggc gccgttgggg agcatggcaa cgctgatgcc gccatagccc
     4021 cacatgaatg ggacataaaa ttggcagggg gaacgatatt cttttgccca gaagctgttg
     4081 ttgtaaagtt cagaccctgt acttaacccg tggtcggagg aatcgcgctg catggtggca
     4141 gcgagcaggt ctggatgcag gatctgttcg ccgttgatct ggcccgattg cgagttgaga
     4201 aaagcggcga tcttggcgat atcgtcctgt gtgtaaaaca tgccgtgact gccaaaggcc
     4261 ctgcctgtgg ggctgttatc ggtgcgcaga gaagagagag tgcctgtgct gagatgcagt
     4321 ggaatgaaga tatcctgtac gagcatgtca aagagatcgg catcaccgcc tgcttgttgg
     4381 cgcagatagg catacatggt ttgggtggcg acgaaggtgg ccgtggattg atagacccat
     4441 tgctcgccag gtttaccgct ggctgggaaa ctgagcgcgt atttcaattt gtcgtcgtat
     4501 ttttctgcaa cgaggaaatt ggtggagatg gccgagaaat cttcatcggt catgtaggtg
     4561 ggggcttcgt aacggccagt ggccatatcg agtgtgtgtt cgagggtgat cgaatcccag
     4621 ttggtggctt cggggagata ttctttgacg aggatgtcac cgacgttctc atcgtacagt
     4681 tgaccgaggc gcatgtaagc catgcctgcc agcaccgatt tggcggtgga ataggatggc
     4741 aggcgcattt cggaacaata ggcatattgt ccgtggcggg tgttgcagtt ggaaatgtag
     4801 ttcacgccgt tgtagaacac accaaagacg gtggtgtgtt cgggcgtggt gcccgatgac
     4861 atgaaggttt cattgacctt tgcgttgggg taatcagaag caagcgcgct catgggtttg
     4921 gtgggcaatt gattcttgag ccagtcgaaa tgcgcggcgg tgactgcttc ggcatttgca
     4981 acttgtgacg gggtgtaagc ggctttgagc tgcccccaca tgtcaaactt gaaatacatg
     5041 caggtttcct gagtcacttg atagcggaca tttgaaatgg tcgtgtcgtt gaaaaggaag
     5101 gtcatcacgc cgttgtggac gcaattctga ttccgctcga tgagggcaaa tggaaaagac
     5161 gcccgcgaaa aaccctggtc acctgattca ttccatgcgc ggccaggagc caccatgtaa
     5221 ttccaggtgg gttgattgga gtacacaagg ctttggtcca cggggatcaa atggctgccg
     5281 ctttgaacga attcaaaatt catctcgggc aggtgcatcc aggcggggtc agcggagtaa
     5341 ccaaagtcat cgcgcagaga ttggaatccg cctgcctgcg cttcgtcggt caattcaagt
     5401 ttgccttgaa aggtgaatgt gggcggtgca gcatcggcgg ggatggcaaa ggcagaatca
     5461 tccacagggc tggaagattc gcccgacatc aattcatcaa aggtgaggac cgtgcgggtc
     5521 gaattgccag aaccagtcaa cgggtcgaag ggagtcgggg tggcggcggg gatgttcggc
     5581 tgctggtcaa ccgtttcctc ctgtgtggcg gagggagggt tggcagggtc agcgtttccg
     5641 ccgccgcctg tcaaaaggga acaagccagg ctgggtagaa tcaaaagcag gaataaatgt
     5701 ttcttcattt gagcctccag tattaaggat atgcgccttt atagcacaac atggggcggg
     5761 catcatccgc cattggtggg aggtgaattt acagtgtcac ggctataatt caatttatga
     5821 tgcgatattt catacgggca atgaacatcc tgttttttgt gctgggcgtt gcggctacgg
     5881 tggttttgtt cgcgttcttc tatctggctg cgattcgtcc ttccacggaa cgcaagtgga
     5941 tgcaaattcc cctgcccgat gaagcgcttg tcaatgtgta tttgagcgac gatggcgatg
     6001 tgcttgcaga tggcgaaaac ggcgggctgt atgaatttta cagcgacccc gaccccgtgt
     6061 ggaaaaaagt aagcgaacct gaagcccagc atcccagcgt aacatgcggc cccatcatgg
     6121 aagacctgta cgaaagcctg ccgcctcccg gtgaagtcag catgatggtg agcgagaatt
     6181 gcccctttgc cgaacaagcc aattatctgg aagtcgccct gctcaaaaac ggcgaaacct
     6241 ggttcctcaa aagctccacc aattcctacg tgggcatcgg tctttttgtc cttcttccag
     6301 ttggcgttct gatcaatatt gtgctgtatg ggatctggct gttctttttg gcgattgacc
     6361 tgatcctcac ccaaagacac aagcggacac aaaccgcatc tgcaccttaa ccgaaatctg
     6421 attcggcgta ctggggcagc ctctccccga tacttgatgg caattgcatc catttgggag
     6481 tctctgaaag ggcgggggaa tataatcaat tcatgaaaac acttcgaatg atcaacggca
     6541 tctttttggg gttgagcctg tgttttgcat tggcgattcc cattttagga atcggctcgg
     6601 cggcgatcaa ctggaatggg atttgctacg ggtttacaga cgggcaggtt ccctgttcct
     6661 ggtgggagta cacccaaaat gaaatgtttt gggcttcgtt catttttctc cccttgctgg
     6721 tgatgacgtt gctcatgtgg ggtctcatga atttgatccg ctgggtcaga cgcggagcgg
     6781 gaacaataaa ctctccccgt cgttttcatg acaaggagaa ttcccatgtt caaaaataaa
     6841 gcaatctttc ttttccttgc cttgtgcctc accgcctgca catcacaacc tgcgttgagc
     6901 gcagaagggg aaaattgggt ggcattttcc gcagaaaagg cacaagccga ccaaatgctc
     6961 gattggctct tcaccaccga tgctgaatat tggtccccca ctgaagcgga tatccatgcc
     7021 ctggagagcg ggttacccgc ctatttacag gagaataagt ccgcgtttta tatgacagag
     7081 actctgatct gggagcgtct tgacgagtac aaccgtcaat atgttggcat tgtccttgac
     7141 ggaaggaagg tcatttatgc caactattta tgtcagcgtg gagaaggaag cgggtggaag
     7201 gagcaattcg ccttcgtggc tgatggaggt gcttgttatt ttcaattcaa gtttgatacg
     7261 tccacgggtg ggttcttcga tttgcaagtg aatggtgagg cttaaatagt aaaaaagtaa
     7321 gactcctaat gtaaatacgt ctatacataa acctgacgca tggagtttta taaaatgaga
     7381 ctttattact ggcattatgg ggtgatcgtg agcagcctgg ccacggcagt attccacatt
     7441 atgtgtttcc cttactttgg atggttcgac ccgatcgttt tgaacggatt tgggtctctg
     7501 gctttgttgg cggcgtattt tttacccatt ccgttcctgc aacagaaccg ccgcgtgaca
     7561 tattgggcca ttgttgccta caccctgttg acgattattt tgtggatcat ccttggtgac
     7621 aagaccttcc agtttgccac cacagctgcc attgggtatt atgccaaagt ggcagagatc
     7681 ttcttgatgg tctttatggg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nngccattgg gtattatgcc aaagtggcag agatcttctt gatggtcttt atgggctttg
     7861 acatgcaatg ggcattgcag cctgcatcaa aaaatgcgga ggctgaccag ggttcttgac
     7921 atcagctgac cgctcgcata caatcagcga gttcgttatc ccaccaaaca tggaaaggag
     7981 gtacgcaaga aaatgaaaac cattaacttg ttcaagaaat ttgtcctgag cgcacctccc
     8041 gcaaatggaa tctagccgcg atcttgtctt gttcgcttgc tttaagtttc caaccgcagg
     8101 gtgcagctcg ccctgcggtt tttgattctg tcaccttgca gggtgatcgt caaaaaatga
     8161 atccactatg aaggcccaaa aggcaaaatg gagaaaaaat gaaccagagt tttcacaccg
     8221 agaacagacg caagctatct gcacacatgg cagatggcga ggtcatgttg ttttttagcg
     8281 gcgaaagcgt tcgaaaaacc gccgacgaga acttcccgtt ttttacaaac cgcaacttcc
     8341 tgtatttaac gggtgtcaag caggagggtt ctgcattact gcttgaaaag aagaacggga
     8401 tcgtccaaga gcgtttgttt gcagccaaac ccgattttgc acaggagatc tggcatggca
     8461 ggcggtttac agaaattgaa ctcaaccaac tcagcggcgt ggagcggatc gaagacattg
     8521 ccaatttgaa cagggcattg aatgaacttc tttccttcca acatatcaca gccctttggt
     8581 tgtgtttcga tgcactcgct tcagagcgtt cgtttgacat cgaacgcgag tttgcaaaag
     8641 ggatccaggg caggcatcct cacgttgtga tcaggaacag ctatccgctt ttggcgaaga
     8701 tgcgaaagat caaggcgccc gaagagatcg aagccattcg caaggccatg agcattaccg
     8761 aggcgggaat ccgtcgtttg atgcgtattt ccaaacctgg cgtgatggaa tatgaacttg
     8821 aagccgaatt taattccgaa cttgcctcac atgggcagcg ccgcacggcg tttccgtcca
     8881 ttcttgcagg cggcgagcgg atcttttatc tgcattactc caaccccatg gcggccatcg
     8941 cagacggtga tttgatcctc tcggatgttg gagcggccta cgatgagtac tgcacagata
     9001 tctcacgcgt ttttcctgcc aacggccgtt tcagtgagcg gcaggcgcag atttacgagg
     9061 tggctctcgc agcaaatcgt gccatcatgg cagaggtgcg cccaggcgtt tcttttcagg
     9121 tcttgaatcc gatttgcaga caggcctctt tcgatgggct caaggctttg ggcttgctga
     9181 atgacattgc cgatatcggt aaatacgtct ggcatggagc agtccatcat gtagggttgg
     9241 atacccatga tgtaggcgga tacgacgagc cgatcgccag ggatatgatc ttcacagtgg
     9301 atgcaggtat ctatgtccgt gagtggggga tcggtctgcg cattgaagat aatgtgcttg
     9361 tcacggccga tggctgcgag aacctgtcag cggcgatccc gaccacgctt gaagagatcg
     9421 aaggttttat ggcgggcgca aaggcgaaaa attgatcact tcaactcgca aaagcgattt
     9481 tgttcaagtg aacgggattc gcctgcatta tctggattgg ggcggaaatg gcccgacctt
     9541 gattttcctc acaggcatgg gaagttccgc ttacatctac ggcggatttg ctccgctttt
     9601 caccgatcag tttcgtgttc tggcccttac ccgtcgcgga catggcgatt cggactaccc
     9661 cgaaacaggg tacgacgccg atacgcttgt ggaagacatc cgtcaattta tggatgcctt
     9721 gcagattgaa aaagcagcat tcgcaggaca ttccattgct ggcgtggagc ttactcattt
     9781 tgcagccacc caccccgaac gggtggagaa attgatctat ctcgatgcac ttgatgaccg
     9841 cagaaaagaa cgggttgtca tggaacagca tccgctcagg aatgtgcaga tcaaaagaga
     9901 ggaaagcatt ccgccgcgta ccctcgaaga atatattgcc aacgtaaaaa aggaagtgcc
     9961 tgagttcgcc agaatctgga gcgcggtatg ggatgaggaa ttcgctcatg tagtggtggt
    10021 gaacgaagaa ggaatcgtgg tggataaaat gcctgcttcc atcgagaaaa tgatggttga
    10081 aaacctcatc aaagactatg ctcccaaagt ggtgaaggct cagatcccga tcctcagttt
    10141 ttatggaaag cgaaaacgca aactgaccga tgattacacc gaggaacaaa aagccgccta
    10201 tgaaaaattc catcgtgatg tgatcgagcc ttttttcaaa tatctggtgg atgagtttca
    10261 aggcaggttc ccgcaggcgc aaattgtggt gatccctgat gggcatcatt actgcttcat
    10321 ggctcaagag gaactggtcc atgctgaaat gcggaaattc ctgcttgcat a
//
LOCUS       DAOQLC010000137        10183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2558_length_10183_cov_26.342220, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000137 DAOQLC010000000
VERSION     DAOQLC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2558_length_10183_cov_26.342220"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3824)
                     /gene="rpoC"
                     /locus_tag="PL000_17990"
     CDS             complement(<1..3824)
                     /gene="rpoC"
                     /locus_tag="PL000_17990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC90827.1"
                     /translation="MSWSYGEVLKPETINYRRLRPEKDGLFCEAIFGPTRDWQCYCGK
                     YKNPRYKGITCEKCGVEVTRSAVRRERMGHITLATPVAHIWYTRRIPSQMGMLLDVSR
                     RNLDRVLYFAQYVVTYVDEEARKKALQRLEDEITVSEREQAAGVNSKIAEIKTKRDQK
                     IAEIKQKQTALEQGYDEGIAEQMDPIIKEGQKLEKLLQSQMGEPAKKAVVFELTGEKI
                     IDANDKVASKHITMIQKSVQKRLESLENELKDKKQREVEELKTQAAAIKAQADQEMEA
                     LRSALENETTDSSNSSSRLRDELLELRPFTFLSEIRYRELKQRWGQVFRADMGAEAFF
                     DILERLDLDKLSEELWHEVRTTKSKQKRKKSTTRLKVVEAFRRSGNSPAWMILTVLPV
                     IPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGAPDVIIRNEKRMLQEA
                     VDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRNLLGKRVDYSGRSVIVVGPQ
                     LKLNQCGLPKSMALELYRPFVIARLVQNNYAANVKGARRLIERNRPEVWEALESVIGD
                     RPVMLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAFNADFDGDQMAVHVPLSQKA
                     VAEARELMLASRNLLKPADGEPIISPSKDMVLGVYYLTMEKKAVHRGDGRAFADMDEV
                     ELAYGLGQVEIHTEIKFRAKTWYDDKGDRLAEPEVRVLNTTVGRVLFNRVLPEQVQFM
                     NEKLDKGGVKDLIAEVYELCGQEVTTHVADAVKSIGFEYATRSGTTLAVADISIPPER
                     KGIIEEALKSVEVVMRDFRRGLLTEQEKNEREIQIWQEITERVGVAVKKHMDPDGNLS
                     IMATSGATKGGFSTISQLAGMRGLMADPSGRIIPMPIRSNFREGLTSQEYFISTHGAR
                     KGLADTALRTADAGYLTRRLVDIAQDIIINEHDCGTHDGIWIRKSDDVAGQSITSRIY
                     SRLVAERILDPKTGEVIAEYNDVITHDLARKIAGAGVTEVKLRSPMTCQLSHGICSKC
                     YGIDLARGELVDLGAAVGIVAAQSIGEPGTQLTLRTFHTGGVASQGASDITTGLPRVE
                     EIFEARKMPKGEAVVAEISGMIHIRQSEKYADMREVRIEHAEMVHDEYVIPEDWKFVA
                     KDETEVQVGDVLANKEKATIIAQHAGRVAVEKKDHKIIVSYEQREEVIEDIPTTSRLL
                     VKDGAHVDAGQPLTEGSLNPHRVLKIQGRDACQMYLMTEVQKVYRSQGQNIHDKHFEV
                     IIRKMLSKVQVTR"
     gene            complement(4092..7127)
                     /locus_tag="PL000_17995"
     CDS             complement(4092..7127)
                     /locus_tag="PL000_17995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNC90828.1"
                     /translation="MNPPSGNPSQTGNTTPLYRPALDKDNMPLPRRVTEVDLEGTRVS
                     RAAYEPTSQRPPQQTPPPQIPRQNNPQFNFPPIDWRKGWGCFLRGLIASAFVLVIVGL
                     CLGSYFVYQYYSIASTLPDVGDLRDRASQFETTRILDRNGQVLYEILDPSAGRRNYIP
                     LSEISPNLVAATIATEDKEFYTNPGFDPIAIIRALWQNYVTGGQGGGASTITQQLARA
                     LILSPEERTQRTVTRKAREIILAAEITRRFTKDEIIELYLNEIYYGNLAYGVEAAAET
                     YFGKSAKDLTLAEASFLAGLPQSPAVYDIYTNPEATLIRQQQVLVLMFEQSTDQECIK
                     VSNSETPICVDPIAATQAADAMKTYQFRVPSFDAKYPHWVNFVRSQLEEQYDAQTIYR
                     SGFVVYTTLDATMQDKAQEFVSGQISNLVDNNTHNGALVAIRPSTGEILAMVGSPDFN
                     NAAISGQINMAVSPTRQPGSSIKPFTYVAAFEKGWTPSTLIWDVPTQFPDGANPPYEP
                     RNYDGKFHGAMTVRIALSNSFNIPAVKALEFVGIYDDPNTPQKDGLIGMAERLGITSL
                     TRNDYGLSLTLGGGDVSLIDMTSAYSVFANSGKRMPPVAILKITDYAGNLIYEYKPAE
                     GEQVIRSEHAYLISSILSDNESRSLMFGPNSQLNLSFPVAAKTGTTNDIRDNWTIGYT
                     PDLVTGVWIGNADYTPMINSTGLSGAAPIWHNFMEFAVPYLTNGAPNWFTRPSGIMDR
                     IVCKLSGTEPGNMCGAQYTEVFASDQPPLQPGQDLVRRIRIDLWTGLQSSDACNGPKD
                     EAVVMNVTDKWARDWLATNPGRDWLENHDLPRNPMYAPERECSANDPQPIIEIDLSDG
                     ETVTSPILNVKGSAAANGGFQGWTLDYGQGAEPGSWSVLTASNTPIKDGSLYLWDMST
                     LPNGIYTLRLTLIGERAEVDDRVTLSLNLPIPTVQVPTLTATPVPTIFIIPTIPTRTP
                     TPVIIIVPPTSTPIPTETPTEFPTETPTETPTESPTPGP"
     gene            complement(7279..7728)
                     /locus_tag="PL000_18000"
     CDS             complement(7279..7728)
                     /locus_tag="PL000_18000"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNC90829.1"
                     /translation="MAGLYFEEFSVGQKFNTVGRTVSEGDIFNFAGLTGDFNQIHTDA
                     AFAAKTQFGQRIAHGLLGLSLATGLIMRTGLLEGTVLAFREINEWKFVKPFYIGDTIY
                     AELTVTETKALPRIGGGALISAIAVKNQSDEICQRGSLNLLVLSKPK"
     gene            7833..>10183
                     /locus_tag="PL000_18005"
     CDS             7833..>10183
                     /locus_tag="PL000_18005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90830.1"
                     /translation="MRDWSLRPGDPLYLTLAADARLCKPDYVNDHIWEVELGATDPER
                     AAISLYTTFGLRARSMRLSLRFTENNVAVTDPNTFFTKPSLRRFFPNFLTLDFVPLEN
                     LQVSTEFWVPESHAVAGRVILTNKSNAVRQIKLEVCAALAPLDGQSIIPTQQQLVNIL
                     AGQTSGIAPVIFMTGGPKHGPGPQPSLLLELELGPGATRQITFAEAALDTVAMSFELA
                     RHSAARPWEAERARIELLDASQVLDIRTGDPDWDAALAFSQRASHGLFFSGNGGLPNA
                     SFVNARHVDNGFSRKGDGADYPPSWNGQSPLEAYYVSSILQGTPHIAKNLLLNFLATQ
                     DEDGEVDNKPGITAQRGKLLAAPLLASLTWKYYQATQDEAFLAEAFPKLIKFFWSWFS
                     SAHDRNRDGAPEWDHILQTGFDDNPVFDVWHPWSQGLDISLVHSPSLEAMLYREAWSI
                     VKIAKRLGKPEEETALVIAQSEILKTSINASWNPNQSFYSYRDRETGLVSTGQVIAKK
                     KGNGNARPKIEFETGVRLLVEIQTKNPAAKRPEVEISEYFTKSKGEVETILGHQFQWR
                     TGGLVATTQKLFTRVGRVSVTGLDEKDKVLVKLIDTTGEDLTLALPLWAGVPEAQQVN
                     ALIGRNLLTADRFDRPFGFPSLAASPNPEADTVSMSVTLPWNQLIGEGLLAYGFRAEA
                     TRLTAHLMNGVIQNLKQNRSFYNRYHAEKGTGIGERNSLHGFAPVGLFMQTLGVTILS
                     PTKVKLEGKNLFPWAVTIKYKNLTISRGLEQTVVTFPNGESVIV"
BASE COUNT         2359 a         2711 c         2719 g         2394 t
ORIGIN      
        1 cgggtgacct gcaccttgct caacatcttg cggatgatga cctcaaagtg cttgtcgtgg
       61 atgttctgac cctgtgaacg atacaccttc tggacctcgg tcatgaggta catttggcag
      121 gcgtcacgac cctggatctt gagtacacga tgggggttga gtgagccttc tgtgagcggc
      181 tggccagcat ccacatgcgc accgtccttt acgagcaggc gggaggtggt gggaatatct
      241 tcaatgactt cttcgcgctg ttcgtaggaa acgatgatct tgtgatcctt cttttccacg
      301 gctacgcgcc cggcgtgctg ggcaatgatg gttgcctttt ctttgttggc aagaacatct
      361 ccgacttgca cctcggtttc atccttggca acaaatttcc agtcttcagg aatgacgtat
      421 tcatcatgaa ccatttcagc atgttcaata cgtacttcgc gcatatcggc atatttttcc
      481 gactggcgga tgtggatcat accgctgatc tcagccacaa cagcttcgcc tttcggcatc
      541 ttgcgggctt cgaagatttc ctccacacgc gggagaccgg ttgtaatatc ggaagcgcct
      601 tgcgaggcca caccacctgt gtggaaggtg cgcagggtca actgggtgcc tggttcacca
      661 atggactggg cagccacaat acccactgct gcgccgagat cgaccagttc gccacgggca
      721 aggtcaatac cgtagcactt tgaacaaatg ccatggctca actgacaggt catgggtgag
      781 cggagtttta cttccgtcac gccagcgccg gcaatcttgc gagcaaggtc atgggtaata
      841 acgtcgttgt actcagcgat cacttcaccg gtcttcgggt caaggatgcg ttctgcgaca
      901 aggcggctgt aaatgcggct ggtgatggac tgaccggcaa cgtcatccga cttgcggatc
      961 catattccat catgggtgcc gcagtcatgt tcattgatga tgatgtcttg ggcaatatcc
     1021 accagacggc gagtgaggta acctgcatct gccgtacgaa gagcagtatc ggcaagaccc
     1081 ttgcgggcgc cgtgtgttga aatgaagtac tcctgcgagg taagtccttc gcggaagttg
     1141 gaacgaatcg gcatcggaat gatgcgtccg gaaggatcag ccatcaaacc acgcatacct
     1201 gcaagctggg aaatggtgga gaaaccacct tttgtcgcgc cggaggtcgc catgatggaa
     1261 aggttgccat cgggatccat gtgcttcttg acggccacac ccacccgttc agtgatttcc
     1321 tgccagatct ggatttcgcg ttcgttcttt tcctgttctg taagcaaacc acggcggaaa
     1381 tcacgcatga ccacttcaac cgacttaagg gcttcttcaa taattccctt gcgttccggc
     1441 ggaatggaaa tatcggcaac cgccagcgtt gtaccggagc gggttgcata ctcaaagcca
     1501 atgcttttta cagcatcggc cacatgggtg gtgacttcct gcccacaaag ctcatatact
     1561 tcagcgatca ggtccttcac gccgcctttg tccagttttt cgttcataaa ctgaacctgt
     1621 tcaggcaaaa cgcgattgaa caacacacga ccaaccgtgg tattcaatac acggacttca
     1681 ggttcggcca gacggtcacc cttgtcgtcg taccaggtct tcgcgcggaa tttgatctcg
     1741 gtatgaattt caacctgacc caagccatag gccaattcca cctcgtccat atcggcgaat
     1801 gcgcgaccat caccgcggtg aacagccttc ttttccatgg tgaggtaata cacacccagc
     1861 accatgtcct tggacgggga aatgatcggc tcgccgtcag cgggtttcag aaggtttcga
     1921 gaagcaagca tcaattcacg ggcttcggcc acggccttct gtgaaagcgg aacatgaaca
     1981 gccatctggt cgccatcaaa gtccgcattg aaagcggtag tgacgagcgg atgcaattgg
     2041 atcgcagacc cttcgataag gatcggctca aaggcctgaa tacccaaacg gtgcagggtc
     2101 ggcgcacgat tcaacataac ggggcggtca ccgatgacac tctcgagcgc ttcccaaacc
     2161 tcgggcctgt tacgctcaat caagcgacgc gcacctttga catttgccgc gtagttattc
     2221 tgcacaaggc gtgcaatgac gaatgggcga tacagttcaa gggccatgct cttgggcaga
     2281 ccgcactggt tcaacttcaa ctgcggacct accacgatca cggagcggcc ggagtaatcc
     2341 acacgtttac caagcaggtt gcggcggaaa cgacccttct tgcctttaag catatcgctc
     2401 aaggatttga gttcacggcg cccacggcgg ctgagggcct taccgcgctg gctgttatcg
     2461 atcaggctgt caacagcctc ctgcaacata cgcttttcgt tgcggatgat gacatccggt
     2521 gcgccgagtt caagcaatct cttcaaacga ttgttacgat tgatcacacg gcggtacaga
     2581 tcattgaggt cggaggtggc gaaacggccg ccgtccaact gaaccatcgg gcgcaggtcc
     2641 ggaggaatga cgggcagaac ggtcaaaatc atccacgccg ggctgttacc agagcggcgg
     2701 aatgcttcaa ccaccttcaa acgggttgta gattttttgc gcttctgctt gctcttggtg
     2761 gtgcggacct catgccaaag ttcttcagag agcttatcga ggtcaaggcg ttcgagaatg
     2821 tcgaagaagg cttcggcacc catatcagca cggaagacct gtccccagcg ttgtttcagt
     2881 tcgcgatagc gaatttcgct gaggaaggta aatggacgca gttccagcaa ttcatcacgc
     2941 aggcgtgagg aactgttgga agaatcagta gtttcatttt ccagcgcact gcgcagggct
     3001 tccatttcct ggtcagcctg ggctttgatg gcagcggcct gtgtcttgag ttcttcaact
     3061 tcgcgctgct tcttatcctt caactcattt tcaagagatt ccagtctctt ctggacgctc
     3121 ttttgaatca tggtgatgtg cttgctggca actttgtcgt tggcatcaat gattttctca
     3181 ccggtcaact caaagacaac cgccttcttg gcaggttcac ccatttgaga ttgaagcaat
     3241 ttttcaagct tctggccttc cttgatgatg ggatccatct gctcggcaat accttcatcg
     3301 taaccttgtt caagcgcagt ctgcttctgc ttgatctccg cgatcttttg atcgcgcttg
     3361 gttttgattt cagcaatctt ggaatttaca ccggcagcct gttcgcgctc agaaacggta
     3421 atttcatctt cgaggcgttg gagggctttt ttgcgggcct cttcgtccac atatgtgaca
     3481 acatactgcg cgaaatacag aacacggtcc aggttacgac gggatacatc gagcagcata
     3541 cccatctgcg aagggatgcg gcgggtatac caaatgtgag caaccggcgt ggccagggta
     3601 atatgcccca tacgttcgcg gcgaacggct gagcgggtga cttcaacacc gcatttttca
     3661 catgtaatgc ctttgtagcg ggggttcttg tatttgccac agtaacactg ccaatcacgg
     3721 gtggggccaa agatggcttc acagaacaac ccatctttct ctgggcgaag gcggcgatag
     3781 ttgatggttt ccggtttgag aacctcgccg tatgaccaag acatgattgt ctgtggtgag
     3841 gcaaggctga tacggagtgc ggttaatccc ttggtttcca ctgagtactc tcctcaaata
     3901 aatagagttc ttttcgggcg tctctgcaaa gcgtgtagtt aacttactgc agagacaaac
     3961 aaaggcaagc gctccgagag gattctctca agcgcttgct gtgtgtgact cggtttttac
     4021 atccaacgaa gtgattatat gcgtgggaga acccctcgtc aagggtgtta atcagtgaag
     4081 agtctccaat tttaaggccc gggtgtgggg gattcggttg gggtttcggt gggtgtctcg
     4141 gtagggaact cggtcggggt ttcggtaggg atgggcgtgc tggtgggtgg gacgataata
     4201 atgaccggtg tgggggtacg ggttgggatg gtggggatga tgaagatggt ggggacagga
     4261 gttgctgtca gcgtaggcac ctgcaccgta gggatgggca ggttgaggct taaggtgaca
     4321 cggtcatcca cttccgctct ttcaccgatg agggtcagtc tcagggtgta gattccgttt
     4381 ggcagcgtgg acatatccca caaatagagt gagccgtctt tgatgggggt attgctggcg
     4441 gtcaaaacgg accatgagcc tggctcagct ccttgaccat aatccagcgt ccaaccttgg
     4501 aaaccaccat ttgcagcagc agaccctttg acattcaaaa tgggtgaggt gacagtttct
     4561 ccatcactga gatcgatttc gataatgggc tgcgggtcgt tcgcactgca ttccctttcg
     4621 ggggcataca tgggattgcg cggcaggtcg tgattttcaa gccagtcacg gcctgggttg
     4681 gttgcaagcc agtcacgtgc ccatttatcg gtgacgttca tgaccacggc ttcatctttc
     4741 ggcccgttac aggcatcgga agattgcaag ccggtccaca ggtcaatgcg gatacgacga
     4801 accagatctt gaccaggctg cagagggggc tggtctgagg caaagacctc tgtatattgt
     4861 gcgccacaca tattcccggg ttcggtgcct gaaagtttgc agacgattcg atccataatg
     4921 ccagatggac gagtgaacca gtttggcgca ccgttggtaa ggtacgggac cgcaaattcc
     4981 ataaagttgt gccaaatggg cgctgctccg ctcaaccccg tggagttgat catgggggtg
     5041 taatcggcat tcccgatcca cacaccggtg acaaggtctg gggtatagcc aatggtccag
     5101 ttatcgcgga tatcgttggt ggtgcctgtc ttcgcggcaa cggggaagga cagatttagc
     5161 tgcgagtttg gaccaaacat caacgagcgg gattcattat cagagagaat ggaggaaatc
     5221 aggtaggcat gctctgagcg aataacctgc tctccttcgg caggtttgta ctcgtaaata
     5281 aggttgccgg cataatcggt gatcttaaga atggcgacgg ggggcattcg ttttccactg
     5341 ttggcaaaca cagagtacgc ggaagtcatg tcaatcaggc tgacatcccc cccacccagc
     5401 gtgagagaca agccatagtc attgcgtgtg agcgacgtga ttcccaatct ttcggccatg
     5461 ccgatcaaac cgtctttttg cggggtattt ggatcgtcat agatgccaac aaactccaga
     5521 gccttgaccg cggggatatt gaaagaattc gaaagtgcaa tacgtacggt catggcaccg
     5581 tggaattttc cgtcgtagtt acgaggttcg taaggcggat tcgccccatc gggaaattgc
     5641 gtgggcacat cccagatcag cgtggagggt gtccaacctt tttcgaaagc agcaacatag
     5701 gtaaacggct tgatggatga accgggttgg cgggtaggac tgacagccat gttgatctgc
     5761 ccggagatgg cggcattatt gaaatcgggt gagccaacca tcgccagaat ctcgcccgta
     5821 gatgggcgaa tggcaacaag tgcgccgtta tgggtgttgt tatccaccag attggaaatc
     5881 tgcccggaga caaattcctg cgctttgtcc tgcatggtgg catccagagt ggtgtacacc
     5941 acaaagccag agcgataaat ggtctgcgca tcgtactgct cttccagctg ggaacggaca
     6001 aaattcaccc aatgcggata ttttgcatcg aacgaaggca cacggaattg gtatgtcttc
     6061 atcgcatctg cggcttgtgt cgctgcaatc ggatccacac agatcggtgt ttcgctgttg
     6121 ctgaccttaa tacactcctg gtcggtgctt tgctcgaaca tgagcaccaa cacctgctgt
     6181 tgacggatca gggtggcttc ggggttggtg taaatatcgt acacagcggg agattgcggc
     6241 agcccggcaa ggaaggaagc ctcggccagg gtgaggtctt tggccgattt gccaaagtag
     6301 gtttcagcag cggcttcgac accataggca aggttgccgt aatagatctc gttcaaatac
     6361 agttcaatga tctcatcctt ggtgaagcgg cgggtgattt ctgctgccag gatgatttca
     6421 cgagccttgc gtgtgaccgt acgctgggtg cgttcttcag gagagaggat caacgcccgc
     6481 gccaattgct gggtaatggt ggaggcaccg ccgccctgcc cgcctgtgac atagttttgc
     6541 cacaaagcgc ggataatggc gatggggtca aacccggggt tggtgtagaa ctctttatct
     6601 tcggtggcaa tggttgccgc gacaagattg ggtgaaattt cagaaagcgg aatgtaatta
     6661 cggcgcccgg cactggggtc gaggatctcg tacaacacct gcccgttgcg atccaaaatg
     6721 cgtgtggttt caaactgcga agcccggtcg cgtaaatcac ctacatcggg aagggtgctg
     6781 gcgatggaat aatattgata aacgaagtac gaacccaaac acaaaccaac aatgaccagt
     6841 acaaaagccg aagcgatcaa accgcgcaga aaacaacccc aacctttgcg ccaatcaatc
     6901 ggcggaaagt tgaattgagg gttattctgg cggggaattt gcggtggggg tgtctgttgc
     6961 ggggggcgtt gtgaagtcgg ttcataagcg gcccggctga ctcgcgtacc ttccaaatcc
     7021 acctcggtga ctcggcgcgg caagggcata ttgtccttgt ctaatgcggg gcggtagagc
     7081 ggggtcgtat ttccagtttg agagggattt ccagaaggtg ggttcaaagg cgcaggggtg
     7141 tgttcccccc gtgtttcttc ttctgatttt ataattcggc gtaatcgatc gttgtcttcg
     7201 ttcatcaggt tggcccataa atctccaatt atcaattatg aattccataa aagataattg
     7261 gagactggtt aataaaaact attttggctt actcaacacc agcaaattta acgaaccacg
     7321 ctggcaaatt tcatcgcttt gattcttcac agcaatggcc gagatcaatg cgccgccgcc
     7381 gatgcgcggc agggctttgg tttcggtgac ggtcaattca gcgtagatcg tatcgccaat
     7441 gtaaaaaggc ttgacgaact tccactcgtt gatctcgcgg aaagcgagaa cggtgccttc
     7501 cagcaatccc gtccgcatga tcaagccggt ggcgagtgac aggcccaaaa gtccatgagc
     7561 gatgcgctga ccgaattgag tcttcgccgc aaaggccgca tccgtatgga tctggttgaa
     7621 atcgcccgta agcccggcaa aattgaaaat atcaccttca ctgaccgtgc gcccgaccgt
     7681 attaaatttt tgcccgacag aaaattcctc gaaataaagt ccagccattt ttagtctcct
     7741 cgtctttttt aacgttacaa cctttgaacg tgtcaacgtt gtaacgatta tacccgtttg
     7801 acctcacacc atttcgcccg tacaattgcc ctatgcgcga ttggtctcta cgccctggcg
     7861 acccgcttta cctcactctg gcagcggacg cccgcctgtg caaaccagac tacgtcaacg
     7921 accacatttg ggaggttgaa ctcggagcca cagaccccga gcgagccgcc atttctctct
     7981 ataccacgtt cggtttacgc gcccgctcca tgcgcctttc attgcgtttt accgaaaata
     8041 acgtcgcagt tacagaccct aacacctttt tcaccaaacc gtcgctgcgc cgtttcttcc
     8101 ctaacttcct aaccctcgat tttgttcccc tcgaaaacct gcaagtttca accgaattct
     8161 gggtacccga atcacatgcc gtggccgggc gggtgattct caccaataaa agcaacgccg
     8221 tgcgccagat caagctcgaa gtctgtgccg cacttgcccc cctcgacggc caatccatca
     8281 tccccaccca acaacaactc gtcaacattc tggcaggaca aaccagcgga atcgcccccg
     8341 tcatctttat gactggcggc cccaaacatg gccccggccc gcaaccctcg ctcctgctgg
     8401 aactggaact tggtcctggt gccacgcggc aaatcacctt cgccgaagca gcgctcgaca
     8461 ccgtcgccat gtcttttgaa ctggcccgcc acagcgccgc ccgcccgtgg gaggcagaac
     8521 gcgcccgcat cgaattgctc gacgcctcac aggttttaga tatccgcacc ggtgaccctg
     8581 actgggacgc cgcgctagcc ttcagccaaa gagcctcgca tggattgttc ttcagcggca
     8641 acggcggcct gccaaacgca tcttttgtca acgcccgcca tgtagataac ggcttctcac
     8701 gcaaaggcga tggcgcagat tacccaccct catggaatgg acaatcaccg ctggaggcgt
     8761 actacgtttc cagcattctg cagggcacac cccacatcgc caaaaatctt ctgctcaatt
     8821 tccttgccac acaggatgaa gacggcgaag tggataataa acccggcatc accgcccagc
     8881 gcggaaaact cctcgccgcc cccctgctcg ccagcctgac gtggaaatat tatcaagcca
     8941 cgcaggatga agccttcctt gccgaagcat tccccaaatt aattaaattc ttctggtcat
     9001 ggttctcctc cgcacatgac cgcaaccgtg atggcgcccc tgagtgggat cacattctgc
     9061 aaaccggttt cgatgacaac cctgtttttg atgtttggca cccctggtcg cagggattgg
     9121 atatttcact ggttcacagc ccgtcacttg aagccatgct gtaccgcgaa gcatggtcca
     9181 ttgtgaagat cgccaaacgc cttggcaaac ccgaggaaga aactgccctc gtcattgcgc
     9241 aatctgagat tctcaaaaca tccatcaatg caagttggaa cccgaatcag agtttctatt
     9301 cctatcgtga ccgcgaaacg gggctggtct caacaggtca ggtcatcgcc aagaaaaagg
     9361 gaaatggcaa tgcccgcccc aagatcgagt ttgaaactgg ggtccgcttg ttggtggaga
     9421 ttcagaccaa aaatccagcc gcgaagcgcc ccgaagtgga aatcagcgaa tacttcacaa
     9481 aatccaaagg cgaagtggaa accattttag ggcatcaatt ccaatggcgc acgggtgggc
     9541 tggtggctac cacgcaaaaa ttattcacac gcgtaggccg cgtgagcgtg accggcctcg
     9601 atgaaaaaga caaggtgctt gtaaaactga tcgacaccac cggcgaagac ctcaccctgg
     9661 cactgccgct ctgggccggt gtgcctgaag cacaacaagt gaatgccttg atcgggcgca
     9721 acctgttgac agcagaccgc ttcgaccgcc catttggatt cccgtcactg gccgcttcgc
     9781 ccaacccgga agcggatacg gtttcgatga gcgtgactct cccctggaat caattgattg
     9841 gcgaaggctt gctcgcatac ggattccgcg ctgaagccac ccgcctcacc gcacacctga
     9901 tgaacggtgt catccaaaac ctgaaacaga atcggtcttt ctacaaccgt taccacgccg
     9961 aaaaagggac aggcatcggt gaacgcaatt cactgcatgg cttcgcaccg gtggggttat
    10021 tcatgcaaac attgggtgtg acgatcctat ctcctacaaa agtaaaactc gaaggcaaaa
    10081 atcttttccc ttgggcagtg acgatcaaat ataaaaatct caccatctcg cgtggactgg
    10141 agcaaacggt ggtaaccttc cccaatgggg aaagcgtgat cgt
//
LOCUS       DAOQLC010000138        10135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2583_length_10135_cov_23.936905, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000138 DAOQLC010000000
VERSION     DAOQLC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_10135_cov_23.936905"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..4710
                     /locus_tag="PL000_18010"
     CDS             448..4710
                     /locus_tag="PL000_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC90831.1"
                     /translation="MNRTKPAPESYQLPRLMRSRPFGVGEDGENVGRTKGTIISSTLK
                     YMLKQVELRTKDVQAVEEAKHELVRRINEAIADPRHHVTLEYLLNEGNVYSAEFDLYF
                     SEITRQVSGQSFDEFHFMRAANSVPEAIAVLVRPLSLRQVYNLLPRFAAKFADTDFRV
                     ISVKGNSAVIQWRCARELQTLPPETHDVFIHESCLFVQGSLSHVPTVVSGDSPARVIE
                     RKCQLWGDDCCEYEFVWEARNLLERLFGKKNSQAVPAIPAASALNERVPELQRKTMDF
                     PPLPERMTFHPYGLDENGKRIVNTDAATLRSVMEYLKECIARNVEAALPVDVPAVLRE
                     RQVAEAQAQALDLLVTKLNAAMPDARFHVTMEDMTRDGNAFSAEFSSFFAIYCNELSG
                     DPKFDFNRGVKMGYLAPWLIRPLTLRRTFIIMPNLVARFFKADIRVLNVTPTSAVLQW
                     RVGDHLLQLPKSMHGRYVYDVCQVFQGVFSEIPRVHSNLPPAQIRETKCQAYGDDCCE
                     WEFVWEAPQTRNLWNIFKARQGGKASEVLFSAVPASPSPELPRLPRKLISHPYGMDAD
                     GRPIKDLNGLFLRVNIDFMLAAVARRAVENLPNGMTAETAVTQAQQAAMENLLEQVNA
                     CLPKSQHLTQEGLLGLGYASFDLGTVIRETCAEIAQVPNFFFHQGFAIVQSVVYLLRA
                     LSIRQVFTGVPRFASKFGNIDLRVVNDSATSAILRWYPDPTLERSSPYTHQHVVNMTC
                     QTIQGSMAYVPPLVAGLPPAQVREIKCRLHGDEYCEWEFTWQLPRPSRYRSVWLGGLI
                     TLLLLANFYFQGTGWQWVNWFALAFFPWLSGWLLNRWSQRGYQLDRREKLLLEQRDAS
                     EQQYDALQKSNAELQLANLTLQEKIAEVTALTETLEQRVVDRTREVEEARKQAEDANR
                     AKSTFLASMSHEIRTPMNGIIGMAGLLFDTRLTPEQREFAETIRSSSDALLTIINDIL
                     DFSKIEAGKMDLESHPFDLRECVESAVDLVALKASEKGLEVGLLIEPNVPEAVLGDVT
                     RLRQILVNMLSNAVKFTEKGEILITVQNETPLLHFTIKDTGIGIPAERLSSLFQSFTQ
                     VDASTTRKYGGTGLGLAISKRLSEMMGGTMWVDSIAGAGSTFHFTVQTPPTTATTTPK
                     LIVPTQLRGKRVMIVDDNETNRRILTLQSENWGMRPVAYATPLEALTAIRSGERFDVA
                     VLDMHMPDMDGISLASEIRKYEAARHLTTPLLMLTSLSSREEMNADLFSAFLTKPVKQ
                     SVLYNALIEALAIEVAAPIQTASQEQQFDHTLAERIPIRILLAEDNAVNQKLALRMLD
                     RMGYRVDVAANGLEVLEALGRQFYDLIFMDVQMPEMDGLEATRAIRVMPSLTRQPRVV
                     AMTANAMQGDREMCLEAGMDDYISKPIQVKELQRAIEEMAIK"
     gene            complement(4810..5958)
                     /locus_tag="PL000_18015"
     CDS             complement(4810..5958)
                     /locus_tag="PL000_18015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90832.1"
                     /translation="MKIALLSEKYTPDLGGLAISAGRMGHLLDSAGHKVRVFAPSPNL
                     PASEKQTLPINGISVTRFGTRKRVDDTLVDWFELIEAEHKREPFDLIHAYFLPMAGFV
                     GAYAGKFLGIPSVVSIRGNDIERAAFDPSKFSHVMYALQNASAVTTNASVLAKKAKAL
                     IEREIHLIPNGIDTNIFKPLERNEMLAEAIGLNVGKKKEERNFVIGFVGELREKKGLA
                     TLLSGYAQTAKEMPVSLLIVGEIREGEDKRVLEEFQAINPQLQITITGHIPHRDLPAY
                     YSLMDIFIHPSLRDGMPNAVLEAMACEKTVIATPVGGMLDVIEDEKNGRMVPVNDAET
                     LAREIKALLNDEQLRKQLGENARKKIQAEFTPQKELEANLRIYKSLQS"
     gene            5970..6260
                     /locus_tag="PL000_18020"
     CDS             5970..6260
                     /locus_tag="PL000_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90833.1"
                     /translation="MAEKCEMIIGYLVPHRCENPALGACVKCGRGFCDEHTNATREGR
                     VCLACQQGLEMPVALPIAAALFTPDDLSTFDRTRRFNDDVDDENGELFSDLS"
     gene            complement(6266..7330)
                     /locus_tag="PL000_18025"
     CDS             complement(6266..7330)
                     /locus_tag="PL000_18025"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC90834.1"
                     /translation="MYSLDTLANEKDDFQKAVLNARAFKPLYVKIKINYGCNLKCAMC
                     KHWRETREAPIPMDRFKEVISELAELGTKKIHFSGGEPMLRPQLPDLVAHATGLGMRV
                     TLTTNGTLINKEKAKALVEAGLRGVNVSIDSPIRKVHEKVRGVEGSFKLTTKAVSLFN
                     KYSHKGKLTVRINTVVSRENYFSLATLPELAHELGADGINLIPVDDHCGEHLSMRKKD
                     IALFNEEIAPYIEKRASELGVNIADEDAFPFGRDESEVKLGRAGRYAFGYYSKFPCYA
                     PWTHSLIDFNGLVYVCCMTREQIPPLGDIRKQSFKEIWEGLAYQNVRQKMHPPSLKPC
                     QRCDDFILENKKIWETVGPY"
     gene            complement(7407..8273)
                     /locus_tag="PL000_18030"
     CDS             complement(7407..8273)
                     /locus_tag="PL000_18030"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90835.1"
                     /translation="MFRKTSQVNEYRVLINDQPWNIFEAGSGTPMLFLHNGGGTLWNW
                     AHQLQYFTDTYRVIAPDLPGFGRSHRPAQPLTLDAYVDGLERLLEILDCPQPILVGNC
                     IGASIALEFALRYPEKVSALTLFNICGGPPMLNPFLRIWSTTQPITAFGVTAHRVMIE
                     AAAHPALRKFGTSLLYADHEPTLHPQLESFLQAQQSDPDLRASLYWLAKGLDSFSIFS
                     QPRQKPVHFPPVLLGWGAKNRTLHPRWADQITDWLKPQEFWLVENAGHMPMYEQPEQV
                     NARLEQFLRVHL"
     gene            complement(8249..8701)
                     /locus_tag="PL000_18035"
     CDS             complement(8249..8701)
                     /locus_tag="PL000_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90836.1"
                     /translation="MIKLIRTNSENTDFIELVRHLDADLAERDGSEHAFYAQFNKIAK
                     IKHVVVAYEDGTPIGCGAIKEYETGTMEVKRMYTTPASRGKGIASRVLSELENWAAEM
                     SCQKCVLETGIKQPEAIALYKKNGYQIIPNYGQYIGVDNSLCFEKLLK"
     gene            complement(8787..>10135)
                     /locus_tag="PL000_18040"
     CDS             complement(8787..>10135)
                     /locus_tag="PL000_18040"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNC90837.1"
                     /translation="CNSKIPTHGVNVMEKKQLSRLILTAIMVFTLACNAVTQMIPSTG
                     QETPTNIPPSPVDTATPAPTAIPPTPTLAVTTMTSEKDGATLVLVPAGEFTMGRGDAS
                     DSSDYAPAHIVYLDAYWIDQTEVTNGMYAQCVQAGGCKRPTDTSSNNNPSYYGNPEFD
                     DYPVIYVEWDMAKAYCKWAGRRLPTEAEWEKAARGTDGRSFPWGEDTISGFENFAMIV
                     GDTTKVASYEKGKSPYGAYDMAGNVHEWVNDWYDYEYYKNSPSSNPQGPDVGRPPARV
                     LRGGSFVEDGFFSLTTTRIWNLDTLIGVGFRCASSDNIATTDSPNQPPAQQVTCADGV
                     VIDYTETPKGVVLEICAFNESYEIPGLAKGAFAVGPNNKFFVYCTNGGDVFAVRIGDT
                     QMQLIGDVKDFYMIRRGEVPEFAFSFEGDHPYKVTVREIKSNETQVFPIPRFVSAP"
BASE COUNT         2223 a         2430 c         2815 g         2667 t
ORIGIN      
        1 tttacacagg tcaatgcctt gaccaggctc gggtatgtgc tgatgggaat attgctgatg
       61 gggaagtttt caagcgcccg tgctgtgtgg acaccataat atacgtcact gggaacttca
      121 cggtcaccta aaaggtcatg ttcaatgcga tggttcactt ttgctccaga gttttgattt
      181 gcgtatccct gtgttgcgag tgactggaac cctgtttcag cttagagtcg gcttgtaagg
      241 ggctggcagg tcacgggtcg gcagggatgc ggatttttat tccaaaattt tggaatgctt
      301 tgacaaaata atgataccat tgtgaaaatc aaattttaaa ggaatttgta gtgtaatctg
      361 ccgcaaaacg gatctggatt tatctgaggt ttgaagttta tgagtatatc ggaacttaat
      421 tcccctttgc agacaaagag cggatctgtg aacaggacga aacctgctcc agagtcatat
      481 caattgccgc ggttgatgag atcgcggccg tttggtgtgg gtgaggatgg agaaaatgtc
      541 gggcgtacca aggggacgat catcagctcc actttgaagt acatgctgaa acaggtggag
      601 ttgcggacga aggatgttca ggcggtggaa gaggcaaaac atgagttggt gcggcggatt
      661 aatgaagcga tcgcagaccc acgccatcat gtgactttgg aatatctgct caacgaaggt
      721 aatgtatatt ccgccgaatt tgatttgtat ttttccgaaa taacacgcca ggtatcgggg
      781 caatcatttg atgaatttca ttttatgcgg gctgccaaca gcgtgcctga agcgattgct
      841 gtgttggtgc gcccgctttc gttgcggcag gtctataacc tgttgccgcg ttttgcggcc
      901 aaatttgccg atacagattt tcgagtcatt tcggtgaagg ggaattcggc cgtaattcag
      961 tggcgttgcg ccagggaatt gcagacgttg ccgcctgaaa cacatgatgt gtttattcat
     1021 gaaagctgcc tgttcgtgca gggatcgctt tcgcatgtgc ccactgttgt gagcggggat
     1081 tcgcctgcac gggttattga acggaaatgc cagttgtggg gcgatgattg ttgtgaatat
     1141 gaattcgtat gggaggcaag aaatcttttg gagcgtttat tcggcaagaa aaattcccag
     1201 gctgttccag ccatacctgc tgcttcggct ttgaacgagc gcgttcccga actccaacgg
     1261 aagaccatgg attttccgcc attgcctgaa cggatgacgt tccatcctta tggattggat
     1321 gagaacggca agcgtatcgt caacactgat gccgccactt tgcgcagtgt catggaatat
     1381 ttgaaagagt gtattgcgcg gaatgttgaa gccgctctgc cagtggacgt gcctgcagtt
     1441 ctgcgcgaaa gacaggtcgc agaagcacag gcgcaagccc tggatttgct ggtgaccaaa
     1501 ctcaatgcag ccatgcctga tgcgcgcttc catgtgacga tggaagatat gacgcgtgac
     1561 ggcaatgcat tctctgccga gttttcatcc ttctttgcca tttattgcaa tgaactttct
     1621 ggtgacccga aatttgattt caatcgtggt gtgaagatgg gctatcttgc gccgtggctt
     1681 atccgcccgt tgactttgcg gcgcactttc atcatcatgc ccaatttggt ggctcgtttt
     1741 ttcaaggcgg atattcgtgt gctcaatgtc accccaacca gtgcggtgct tcagtggcgg
     1801 gtgggcgatc atctgttgca gttgcccaag tccatgcatg gacgatacgt atatgacgtt
     1861 tgccaggtgt ttcagggagt gtttagtgaa attccgcgag tccattccaa cttgccgcct
     1921 gcgcagattc gtgaaacgaa atgtcaggca tatggcgatg attgttgtga atgggaattt
     1981 gtttgggagg ccccccaaac gcgcaatttg tggaatattt tcaaagccag gcagggcggg
     2041 aaggccagtg aggtgctttt cagcgccgtc cctgcttcgc ccagccccga gcttccacgg
     2101 ctgcctcgaa aattgatttc tcatccctat ggcatggatg cggatgggcg gccgatcaag
     2161 gatctgaacg gtctcttcct gcgtgtaaat attgatttca tgctggcggc tgtggcgcgt
     2221 cgtgcagttg agaatctgcc caatggcatg accgcagaaa ctgcagtgac gcaggcgcaa
     2281 caagccgcaa tggaaaattt gctggaacag gtcaatgcct gcctgcccaa atctcaacac
     2341 cttacgcaag aagggttgtt ggggttgggc tatgcatcct ttgacctggg tacggtcatc
     2401 cgtgagacat gtgccgagat cgcccaggtg ccgaactttt tcttccatca agggttcgcc
     2461 atcgtgcaaa gcgtggtgta tctcctgcgc gcgttgagca tcaggcaggt ctttaccggg
     2521 gttccgcggt ttgcctcaaa atttggaaac attgatttgc gtgtggtgaa tgacagtgcc
     2581 acttcggcga tcctgcgctg gtatcctgat ccaactttgg agcgttcatc gccgtacacc
     2641 caccaacatg tggttaatat gacctgccag acgattcaag gcagcatggc gtatgtgccg
     2701 cccttggtgg ctggcttacc gccagcgcaa gtgcgtgaga tcaaatgtcg tttgcatggt
     2761 gatgaatatt gtgaatggga attcacatgg cagcttccgc ggccatcaag gtatcgcagt
     2821 gtctggctgg gtggtctgat caccttgctt ttactggcta atttttattt tcaaggcacg
     2881 ggctggcaat gggtgaactg gtttgcgctg gcgtttttcc cctggttgag cggctggctg
     2941 ctcaaccgtt ggtcacagcg cggataccaa ctcgatcggc gcgaaaaact tttgcttgag
     3001 cagcgcgatg cctcagaaca gcaatatgat gccctgcaaa aatcgaacgc tgaattgcaa
     3061 ttggcaaacc tgactctgca ggaaaagatc gccgaagtga cggcactgac cgaaacactg
     3121 gaacagcgtg tggttgaccg tacgcgtgag gttgaagaag cccgcaagca ggccgaagat
     3181 gccaaccgcg ccaagtccac cttcctggcc agcatgagcc acgagattcg taccccgatg
     3241 aacggcatca tcggcatggc ggggctgctt ttcgatacgc gcctcacccc tgaacaacgt
     3301 gaatttgcag agaccattcg cagcagcagc gatgccctgc tcaccatcat caatgacatc
     3361 cttgatttct ccaagatcga agctggaaaa atggatctcg aatcgcatcc cttcgatttg
     3421 cgtgaatgtg ttgaaagcgc agtggatctg gtggcattga aagccagtga aaagggactt
     3481 gaggtgggtc tgctcattga gccaaacgta cccgaagccg tgctgggtga tgtcacccgt
     3541 ttgcgccaga ttcttgtcaa catgttgagc aatgcggtca agttcactga aaaaggcgag
     3601 atcctcatca ccgtacaaaa cgaaactccg cttctgcact tcaccatcaa agacacaggc
     3661 attggcattc ccgctgagcg cctatcctcg ctctttcaat cttttaccca ggtggatgcc
     3721 tccaccacac gcaaatatgg cggcaccgga cttgggcttg ccatcagtaa acgtctttct
     3781 gaaatgatgg gtggaacgat gtgggtggat agcattgccg gggctggttc caccttccac
     3841 tttacggttc aaaccccgcc gacaactgcc acaacaaccc cgaaattgat cgtcccgaca
     3901 cagctgcgcg gcaaacgcgt catgatcgtg gatgataacg agaccaatcg ccgcattctc
     3961 accttgcaat cagagaactg ggggatgcga cccgtggcat acgcaacccc gctcgaagca
     4021 ctgaccgcca tccgcagtgg cgaacgcttt gatgtggcgg tccttgacat gcacatgccc
     4081 gatatggatg ggatttccct tgcttcggag atccgtaaat acgaggccgc ccgccatttg
     4141 acaaccccgc ttctcatgtt aacttccctt tcctcgcggg aagaaatgaa tgcggatcta
     4201 ttttccgctt tccttaccaa gcccgtcaaa caatctgtgt tgtacaacgc attgatcgaa
     4261 gcgcttgcca ttgaagtggc tgctcccatc caaaccgcat cacaggaaca gcaattcgat
     4321 cacaccctgg ctgaacgtat tcccattcgt atcttgctgg ccgaagataa cgccgtcaac
     4381 caaaaactgg ccttgcgtat gttggaccgt atgggatacc gtgttgacgt tgcagctaat
     4441 gggttggaag tgcttgaggc cttgggcagg caattctatg accttatctt catggatgtg
     4501 caaatgcccg aaatggatgg tcttgaggcc acccgcgcca ttcgggtgat gccaagcctt
     4561 acccgtcagc cccgtgtcgt tgccatgacc gccaacgcca tgcaaggtga ccgtgaaatg
     4621 tgcctcgaag caggcatgga cgattacatc agcaaaccga ttcaagtgaa ggaattgcaa
     4681 agagccatcg aagaaatggc cataaaataa aaatatcctc cccaattttc aaaaatgcag
     4741 aattggggag gatgcttttt aagcgattcg ccgtatgctt ttttaccaga tcgctgaccg
     4801 acgttttact tagctctgaa gtgacttata gattctcaga tttgcctcga gttctttttg
     4861 cggtgtgaac tctgcctgga tcttctttcg agcattttcc cccaactgct ttcttagttg
     4921 ctcgtcattc aacaaggctt tgatttcccg cgccaatgtt tctgcgtcgt tgacggggac
     4981 cattcggcca tttttttcat cttcgatcac atccagcatg ccgccaacag gggtggcaat
     5041 gacggtcttt tcgcaggcca tggcctccag cactgcattg ggcattccgt cgcgtagtga
     5101 tgggtgaatg aagatatcca tcaaggaata ataggcaggc agatctctgt gggggatatg
     5161 ccctgtaatg gttatctgta attgtgggtt gatggcctga aattcttcaa ggacgcgttt
     5221 gtcttcgccc tctcgaattt caccgacgat caacaacgaa acgggcattt ccttcgcggt
     5281 ttgcgcgtag ccagagagaa gggttgctaa tcctttcttt tcgcgcagtt ctcccacaaa
     5341 gccaatcaca aagtttcttt cctctttctt ctttcctacg ttcaacccaa tcgcttctgc
     5401 cagcatctcg ttccgctcca gcggtttgaa gatgttggta tcgatcccgt tggggatgag
     5461 gtgaatttcc cgttcaatca atgccttggc tttcttggcc aatacgctgg cattggtcgt
     5521 cacggcgctg gcgttttgca gggcgtacat cacatgtgaa aacttcgacg ggtcaaaggc
     5581 ggcgcgttca atgtcgttgc cgcggatgga gacgacgctt ggaatgccca gaaattttcc
     5641 tgcatacgca cctacaaatc ctgccatcgg aaggaaatag gcgtggatta aatcaaacgg
     5701 ttcgcgctta tgttctgctt cgataagttc gaaccaatcg accaaagtat cgtctacgcg
     5761 tttgcgtgtg ccgaagcggg tgacgctgat tccgtttata gggagagtct gtttttcgga
     5821 ggctggcagg tttgggcttg gggcaaagac gcggacttta tgtccagcag agtcaagcag
     5881 atgccccata cgtcctgctg agattgcgag accgccgagg tctggtgtat acttttctga
     5941 aagcaatgca attttcatga ggtgtctcaa tggcagaaaa atgtgaaatg attatcggtt
     6001 atttggtgcc gcatcgttgt gaaaaccctg cactgggagc atgtgtgaag tgcgggcgcg
     6061 ggttttgtga tgagcataca aatgccaccc gggaaggacg tgtgtgcctt gcctgtcagc
     6121 aggggttgga gatgcctgtt gccttgccga ttgccgccgc gttgttcacg cctgatgatc
     6181 tgagtacctt tgatcgcaca cgtcgtttca atgacgatgt ggacgatgaa aatggcgagt
     6241 tgttttctga tctgagttga ttgagttaat acggtccgac cgtttcccag atttttttat
     6301 tttccaaaat aaagtcatcg cagcgctgac agggtttgag ggagggcggg tgcattttct
     6361 ggcgcacgtt ttggtaggcc agcccttccc agatctcctt gaatgattgt ttgcgaatgt
     6421 cgcccagcgg ggggatttgt tcgcgggtca tgcagcacac atacacgaga ccattgaaat
     6481 caatgagact gtgcgtccac ggggcatagc aggggaattt gctgtagtag ccgaatgcat
     6541 acctgccggc gcgtcccaat ttgacctcgg actcgtccct gccaaatgga aatgcatctt
     6601 catctgcaat gttgactccc aattcgctgg cgcgtttttc gatgtaggga gcaatttctt
     6661 cgttgaatag tgcgatgtct ttctttcgca tggagagatg ttcgccgcaa tggtcatcca
     6721 cagggatcag gttgatcccg tcggcgccga gttcgtgggc gagttcgggc agggtggcaa
     6781 ggctgaaata attttcgcgg ctgaccaccg tgttaatgcg gacggtcaat tttcccttgt
     6841 gagaatattt gttgaaaagt gagaccgctt tcgttgtcag cttgaacgag ccttccacac
     6901 cgcgtacttt ttcatgcact ttgcgaatcg gcgaatcgat ggaaacgttc acgccgcgta
     6961 accctgcttc gacgagggct tttgcttttt ccttgttgat gagtgtgccg ttcgtggtga
     7021 gggtgacgcg cattcccagc cctgtggcgt gtgcgacgag gtcgggcagt tgcgggcgca
     7081 gcatgggttc accgccagag aaatggatct tctttgtgcc cagttcggcc aattcgctga
     7141 tgacttcctt gaacctgtcc atggggatgg gcgcttcgcg ggtttcgcgc cagtgcttgc
     7201 acattgcgca tttgaggttg cagccatagt tgatcttgat cttgacataa agcggcttga
     7261 acgcgcgcgc gttcaaaacc gctttttgga aatcatcttt ttcgtttgca agggtatcga
     7321 gactgtacat ggattctcta cgggtaaaag ggaattgaat ttaatccatt gtaacacttt
     7381 gaaaattttc cacctatcat ctatttttaa agatgaacac ggaggaactg ctccagcctt
     7441 gcgttgacct gttcgggttg ttcgtacatg ggcatgtgac cagcattttc gacaagccaa
     7501 aactcctgcg gtttgagcca gtcggtgatc tgatcggccc agcgcggatg cagggtgcgg
     7561 ttttttgctc cccaccccag caagacgggc ggaaaatgaa ctggtttttg ccgtggttgg
     7621 ctgaaaatgc tgaaactatc caaacccttt gcgagccaat acaacgaggc ccgcagatcg
     7681 gggtcgcttt gttgggcttg taaaaaactt tcgagctgtg gatgaagggt cggttcatgg
     7741 tccgcgtata gcagggatgt gccaaacttg cgcagagcgg gatgggcagc cgcttcgatc
     7801 atgacccgat gggctgttac cccaaatgcg gtgatgggtt gcgtagtgga ccagattcgt
     7861 aaaaatggat tgagcatggg tggaccgccg cagatgttga aaagcgtcag ggctgatact
     7921 ttttcgggat accgcagggc aaattcgagg gcgatggatg cgccgatgca atttcccacg
     7981 agaatgggtt gggggcagtc caaaatttcg agcaggcgtt ccaatccatc gacgtaggca
     8041 tccagggtca ggggttgggc aggacgatgc gagcggccaa agcccggcag gtctggtgca
     8101 atgacgcggt aggtatcggt gaaatattgg agttgatgcg cccaattcca gagcgttcca
     8161 ccgccgttgt gcaggaaaag catgggcgtc cctgaaccag cctcgaagat gttccaaggc
     8221 tggtcgttga tcaggacgcg gtattcattt acttgagaag tttttcgaaa cacagactgt
     8281 tatcaacccc gatgtattgc ccatagtttg ggatgatctg atagccattc tttttataaa
     8341 gtgcgattgc ttcaggttgt ttgatgcccg tttctaaaac gcatttttgg caggacattt
     8401 ctgccgccca gttttcaagc tcggacaaaa cgcgggatgc gattcctttt ccacggctgg
     8461 cgggggtggt gtacatccgc ttgacttcca ttgtgcctgt ttcgtattcc ttgatcgcgc
     8521 cgcagccgat gggcgtgccg tcttcataag cgacgaccac atgtttgatt tttgcgatct
     8581 tgttgaattg tgcgtaaaaa gcgtgttcgc tgccatcacg ttcggccaga tcggcatcaa
     8641 ggtggcggac gagttcgata aagtctgtgt tttctgagtt ggttcttatt agtttgatca
     8701 tgataatttg taaaatggat gatgaatgcc cgccgacagc agatcgtgag caacggtcgg
     8761 cgggcgtgga gtgcgataag gtatttttat ggtgccgata caaaccgtgg gatcgggaag
     8821 acttgtgttt cgttgctctt gatctcccga acggtgactt tgtatggatg gtcaccttcg
     8881 aacgagaagg caaattcagg aacctctcct ctgcggatca tgtaaaagtc tttgacgtcg
     8941 ccgatcaact gcatctgtgt atctccaatg cggacggcga atacgtcgcc gccgttggtg
     9001 caatagacga agaacttgtt atttgggccg acagcaaagg ctccctttgc caacccgggg
     9061 atctcataac tttcgttgaa ggcgcaaatt tccagcacga cgcctttggg ggtttcggtg
     9121 tagtcaatga ccaccccatc ggcgcaagtg acttgctggg cgggtggttg attgggcgag
     9181 tctgttgttg cgatgttgtc tgaactggcg cagcggaatc cgaccccgat cagggtgtcg
     9241 agattccaaa tgcgggtggt tgtgagcgag aagaatccat cttcaacgaa cgatccgcct
     9301 ctgagcacgc gcgctggcgg gcggcctaca tcaggtccct gtgggtttga ggagggggag
     9361 tttttgtaat attcataatc gtaccagtcg ttgacccatt catgtacatt gcctgccatg
     9421 tcgtaggcgc cgtaggggct ttttcctttt tcgtagctgg ctactttggt ggtgtcaccg
     9481 acgatcatgg cgaaattttc aaagccactg atcgtatctt cgccccaggg aaatgacctg
     9541 ccatcggtgc cgcgtgcagc cttttcccat tcggcttcgg tggggagtct gcgccctgcc
     9601 catttgcaat aggccttcgc catgtcccat tccacataga tgacagggta gtcatcgaat
     9661 tcggggttgc catagtaact ggggttgttg ttggaactgg tgtctgtggg gcgtttgcat
     9721 ccgcctgcct gaacacactg ggcatacatg ccattggtga cttcggtctg gtcgatccaa
     9781 taggcatcga ggtaaacaat gtgcgcgggg gcgtagtcgc tgctatctga ggcatcacct
     9841 ctacccatgg taaattcccc ggctggcaca aggaccagag tggctccatc cttttcactg
     9901 gtcatggtcg tgacggcgag ggtgggagtg ggtgggatcg cggttggcgc aggggtggcg
     9961 gtatccaccg ggctgggcgg gatgttcgtt ggggtttcct gccccgtaga aggaatcatt
    10021 tgcgtgacgg cattacaagc aagcgtgaaa accattattg cggtcaagat cagacgggaa
    10081 agctgtttct tctccattac attgactcca tgagttggga ttttggaatt acagg
//
LOCUS       DAOQLC010000139        10082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2594_length_10082_cov_20.203251, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000139 DAOQLC010000000
VERSION     DAOQLC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_10082_cov_20.203251"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..568
                     /locus_tag="PL000_18045"
     CDS             218..568
                     /locus_tag="PL000_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HNC90838.1"
                     /translation="MNDQAVEQEIQAKGLTAPRVTPADIEANIASEHYFTAEQGAEAA
                     APSYGDKYPQALGLLTFCVLVLRNGFTVTGESACASPENFDAELGRKIARQNAVAKIW
                     PLMGYELKCRLVSV"
     gene            777..1328
                     /locus_tag="PL000_18050"
     CDS             777..1328
                     /locus_tag="PL000_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90839.1"
                     /translation="MNTSADPFADFVEPSPQAEKSLYPYQEGKAIAKVSYSHDAMIDM
                     LIANPRISQGELANYFGYTQPWISLVMSSDAFKARLAARREELIDPAIRLSIEERFHA
                     VVTRSQEVLLAKLSQPANQVSDNLALRAMELGAKALGLGTGQQAAPSVPAADHLASLA
                     HRLIDLQQSARAQVYEAEAINAT"
     gene            1575..3044
                     /locus_tag="PL000_18055"
     CDS             1575..3044
                     /locus_tag="PL000_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90840.1"
                     /translation="MAQAESIKLTAELVEALAGTFLSPMYDNPQPTPDFHRQGWALYC
                     SSAELAAIAAPRGHAKSTAFTHDYGLGVLLFRIQSYMVIASATEDLAIGHLSDMAMEL
                     RDNEDLRREFQIEKLLVDARTEIIVLFKDGYQAKVVAKGSGQKMRGMKWRGKRPGLII
                     ADDLEEDEQVENKARREKFLRWVNRALIPCRRRGGLVRMHGTILHEESVLSKAMRSKA
                     WSSLFFSAHSSMDDFSNILWPGAFTEDRLRAIRQVFLEDGDAAGYSQEYLNKPRDNSE
                     AYLRSSDFLPMGDADYAAKKIYKIGADFAVSKADTANRTSFTVGGQSLDGLVHIVDQR
                     VDRWSTNEWIDVMFQLAEEYTVDEFVVEGGVIWKAVEEIIFREMRERNLYLPIRVINP
                     VKDKAVRARPFQKRLRGQGLRFNKKAEWYENYEDECLSFVPGSEARLDDQFDSTAILV
                     KGFDNQASPEEEDFFSDEEVEFYTHDPRIFMNRCQTTGY"
     gene            3046..5187
                     /locus_tag="PL000_18060"
     CDS             3046..5187
                     /locus_tag="PL000_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90841.1"
                     /translation="MLELETSLSTSDLVESVNIAELLMEGERRQIALLVQSDFDADVT
                     GREDWERKMKGALELALQVTEPKSFPWQGAANVKFPIITIAALQFHSRAYPAMVPGPD
                     LVRCRTFGPDPTGEKEARATRVANHMSYQILEEDESWEEDHDRVLLTVPIIGCAFKKI
                     YFNQELGHNVSEYVPAKDIYIPYFAKSVEAAARWTHVVETSKNELISYQRRGFFCEQE
                     PLSAPVDPLKPSIGQPVERMLGTEISPRQEDLPYELLEQHRYLDLDGDGYEEPYIVTM
                     IRGSGQLCRIVARYTSQDVKRNSAGEIYSITCQQNLQKYSCIPSPDGGIYDLGFGILL
                     QPLNDSINTIINQLLDAGTLANTAGGFLGRGAKIRSGDTAFSPFEWKRVDSLGDDLRK
                     SIIPLDVREPSAVLFQLLGLLIDYSERVAGSMESQVGISPGQNTPAETTRTVVAEGQK
                     VFIGIFKRLYRAMKGEFRKLYNLNQVYLQEGTTVYYDASGERQKILSLDYLPDAKTVC
                     PMADPNMVTDMQRIQQAQFLKQSAMTTAGYDLQAVERRFLEALKISDIRTVYPGPDKV
                     PPLPNAKLEIEKMKVQERSQAQRMEMQMAALQLLGEAELNQARVQELRAKAVLHLSQA
                     DGVKSGHEIALLEAQIGAAKAHQESLLKSAQLILDSLHKSEGRVNDARQSRGTPRPDG
                     GGNPGMAEPPSNQVLQGLLGGQGEGDAGGLG"
     gene            5295..5615
                     /locus_tag="PL000_18065"
     CDS             5295..5615
                     /locus_tag="PL000_18065"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90842.1"
                     /translation="MNTSGIQPAGHVILVKPKVVEETTKSGIVLATATQRSREHMGQT
                     EATVIALGPTAYSDQAQPWCAVGDQVIFARYAGTERVGADGEVYRLINDLDVKAIYTG
                     GDNV"
     gene            5608..6405
                     /locus_tag="PL000_18070"
     CDS             5608..6405
                     /locus_tag="PL000_18070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90843.1"
                     /translation="MSDLETPNMPDAPDPAEAEARLIGWRPREEYTGPEERWKTAEEF
                     LEEGKRINGFLRKDLDKLRSELARRDASLAEMKQAIQEFSAHHEKTVKAEYERAVRDL
                     RAARKQALRENDGETVVQLEEQLEALGPPPTPRRPTQPAPNLAQDPVWVDWLGENDWF
                     QKDPKRRGLANGYSEILRAEQPELVGRPFLDEVTRRVQADFPELFGQARSRPQQVLTG
                     GRGSAAPSGRKSYADLPAEAREACDKFVRLGLVKSKEDYVKDYFGVA"
     gene            6402..6851
                     /locus_tag="PL000_18075"
     CDS             6402..6851
                     /locus_tag="PL000_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90844.1"
                     /translation="MMETVRPTVQKPTEQVRNEAERVRRPERIPFGVPQSRLNVPLQI
                     PGYHLHFINDSPGRLQQAQLGGYTFVAPSEIGLSGAETQYKVLAGRNEDGSAMYAYLM
                     KIELEFYEEDQRFHQKTVDQFDEAIRRGKIEERGNENRYGKMNSQTF"
     gene            6864..7502
                     /locus_tag="PL000_18080"
     CDS             6864..7502
                     /locus_tag="PL000_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90845.1"
                     /translation="MANANTPIGLKPVRTNTAAGWDGSANLYYIPSTDTNAYRIGDAV
                     VSVAGGDTLTGAPAVTLAGTRNAAMTTGSLRGAIIGIGTAAGTAGMAVPLAADPNALG
                     IVSIPATKTQNYFVWVTDDPGQVYEIQADSIASTAFNKNCPLFVANDPSVPTFNSASY
                     AQGSQADTTQALPLKILGAPFRPDNDLTSPGTYARIFVMINQHELAGNTAGV"
     gene            7535..8461
                     /locus_tag="PL000_18085"
     CDS             7535..8461
                     /locus_tag="PL000_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90846.1"
                     /translation="MAGPIMTSNHPRFLWPGVKGIWGRTYDDHQTEYTDLFDIETSDK
                     AYEEFVQITGFGLAPVKAQGADAVYDSEVQGPISRFVHLAYALGYIVTHEEIQDNLYL
                     EVSKTRASSNARAFRQTKERVAANIYNRAFSSSAAYFGADGVALCSTAHPNTSGGTYQ
                     NKPTIDADLSEASLEDLLILMMQATDDRGLLINLMPKSLHIAPQNWFNANRILHSVYQ
                     AGNANNDINVLRATGAFPGGIKLNHYFTAPQAWFVRSNIERGKGMVFLQREPISFDQD
                     KDFDTKNAKSLGYERYSCGFVDPRAVYGVNGP"
     gene            8578..9318
                     /locus_tag="PL000_18090"
     CDS             8578..9318
                     /locus_tag="PL000_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90847.1"
                     /translation="MGTPTRFKNGVTNAAVNSIFGNLPIPDVTINGLTEYFNDFHTYA
                     AGDWTVTASGSGSSAISAGLGGRLALTWGTSGTQGNTLTSGGFSFNAASTSANGSQFW
                     FSSGIINPANASAPNFLVGAIKGNPTSISDGVYFTKAAAGLPWRINIKAAAASTTTVT
                     LPTSTNAANNSVQELGYYYDGRANPTLFIYVNDVMVGTFGANGSLGTLANLPASTILL
                     NPSAAIGTAAGPLLVDYLFCACEIPGRL"
     gene            9516..9851
                     /locus_tag="PL000_18095"
     CDS             9516..9851
                     /locus_tag="PL000_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90848.1"
                     /translation="MNEVHVCNGFGYRMPVSGTALTTSAPATGNAIFKASPGSTFQLI
                     AAAAATAIIEVTNEDATAAGTNSNWITMGTITLAGAGTDGFMTQACWKWVRARITAAS
                     AATSVLMGS"
     gene            9853..>10082
                     /locus_tag="PL000_18100"
     CDS             9853..>10082
                     /locus_tag="PL000_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90849.1"
                     /translation="MTISLVDKVAGTTPAGGGGAVSSVNSRTGDVVLTAADVGATLLT
                     GSNTGDETTTSIGTLISGATEKPTPIDADELS"
BASE COUNT         2290 a         2803 c         2832 g         2157 t
ORIGIN      
        1 gggggagggg caaaaaaata ccgactaaat tttatattat ccccctccta attttgcaac
       61 ctgatttgaa gtatggccat atcattgttt cgttatccgg ccacagctcg gttatgcccc
      121 tcgcacccgc gcacagccct tgcctccacc ctccccatcg tgtaagctgg tggggcaggt
      181 tgcagagcgc ggccactttt tagagtagag gacagatatg aatgaccaag cagttgagca
      241 ggaaatccag gccaagggcc tgacggcgcc gcgcgttacg cctgcggaca tcgaagcgaa
      301 tatcgccagc gagcattact ttactgccga gcaaggggcc gaagctgccg ccccttcata
      361 tggggacaag tacccacaag ccctcggcct cctgaccttc tgcgtcctcg tcctgcgcaa
      421 tggattcacc gttaccggtg agtcggcgtg tgccagcccg gagaattttg atgcagaact
      481 tggccgcaag atcgcacgac agaatgccgt tgccaagatc tggccgctga tgggatacga
      541 gctaaagtgc aggctggttt ctgtctgaag ggacaagtcg atgagcgcac aggtggattt
      601 cagagagttg gaaaaggaac ggactttcgg gcagggccag gacctgcgtg cggggctgaa
      661 ggcgatgctg ggagaagacg ggtacgccag atttcttcag cactatggaa cttccaaggc
      721 aacgctgcgg ggaccctgtg caagtgggtg tgcttgcaag gaaaggagtc aggacaatga
      781 acacttctgc tgatcccttt gccgatttcg tcgagccctc gccgcaagcc gaaaagtctc
      841 tctatccgta ccaagaaggc aaggccatcg caaaggtcag ttattcgcat gacgcgatga
      901 ttgatatgct gatcgccaat cctcgtattt cgcagggaga gctggccaac tattttggct
      961 acacgcagcc ctggatcagt cttgttatgt cgagcgatgc ctttaaggcc cggctggccg
     1021 cccgtcgcga ggagctgatt gatccagcca tccgcctctc catcgaggag cgctttcatg
     1081 ctgtggtgac gcggagtcaa gaggttcttc tcgctaagct cagccagccc gccaaccaag
     1141 tctccgacaa cctggccctc cgtgcaatgg agttgggggc gaaggccctc ggtctgggga
     1201 cagggcagca ggcagcccct tctgttcccg ctgccgatca tctcgccagc ttggctcatc
     1261 gcctcattga ccttcagcaa tctgcccgtg cacaggtcta cgaagcggag gccattaatg
     1321 caacatagtg tgtgcgcctc cgggaccatc gacctggatc tcaggcagct tgctctggaa
     1381 gaacttctgg agcactctca cggggtcatc atagaaatcc ctcgccgcac gatgattcgg
     1441 gtagagctgg aattgattcg cattgctaaa gaaggtggta catcagattt cctggaaggt
     1501 gctctcagta caatcagatg gcttagcaac ggtggtccct ccccttacga agctataatg
     1561 gggcttctgt cctgatggct caagcggaat ccatcaagct gacggctgaa ctggtagagg
     1621 cccttgcagg taccttcctc agcccgatgt acgacaatcc gcagcccaca cctgacttcc
     1681 accgtcaggg atgggcgttg tattgcagct ccgcagaact cgctgccatc gctgccccgc
     1741 gcgggcacgc gaagtccact gccttcaccc acgattacgg attgggtgtt cttcttttcc
     1801 gcatccagag ttacatggtg atcgccagtg cgacagaaga cttggcgata ggccacctct
     1861 ctgacatggc tatggagctt cgcgacaacg aggatcttcg cagggaattc cagattgaaa
     1921 agctccttgt tgatgccagg acagagatca tagtcctctt caaggatggc taccaggcaa
     1981 aagttgtggc aaagggttct ggccaaaaga tgcgggggat gaagtggcga ggtaagcgcc
     2041 ctggcctcat catcgcagac gacctggagg aggatgaaca ggtcgagaac aaggctcggc
     2101 gggagaagtt tctccgctgg gtcaaccgag ctctcatccc ctgccgccgc cgtgggggac
     2161 tggtccgtat gcatggaact atcctgcatg aagaatccgt cctttccaag gccatgcgca
     2221 gtaaagcttg gtcttccctt ttcttcagtg cccattcttc gatggacgat ttctccaata
     2281 tcctgtggcc cggtgctttt acagaagacc ggctgcgggc tattcgacaa gtctttctgg
     2341 aggacgggga tgcagcgggc tacagccagg agtaccttaa caagccccgc gacaactctg
     2401 aggcgtatct ccgcagcagc gactttcttc ctatgggcga tgccgactat gcagccaaga
     2461 agatttacaa gatcggggcg gattttgcgg tcagcaaagc agacacagcg aaccgcacta
     2521 gtttcactgt tggcggccag tctctggatg gcctggttca tattgtcgat cagcgggtag
     2581 atcgctggag cacaaacgaa tggatcgatg tgatgttcca gcttgcagag gaatacactg
     2641 tcgatgagtt cgtggtggag ggcggggtta tttggaaggc ggtcgaggag atcattttca
     2701 gggagatgag ggaacgcaac ctttatcttc cgatccgcgt tatcaaccct gtcaaggaca
     2761 aagctgtccg cgcccgccca tttcagaaga ggcttcgcgg tcagggtctc cgtttcaaca
     2821 agaaggctga gtggtatgag aactatgagg atgagtgcct cagtttcgtc cctggttccg
     2881 aagcccgcct ggacgaccag ttcgattcta ccgctatcct ggtgaagggt ttcgacaatc
     2941 aggcatctcc cgaggaagag gatttctttt ctgacgaaga ggtagagttc tacacccacg
     3001 atccgcgcat cttcatgaac cgttgccaga caaccggata ctaatatgct tgagctggaa
     3061 acctccctaa gtacctccga tcttgtcgag tcggtcaaca tcgccgaact tctgatggaa
     3121 ggggaaaggc ggcagattgc ccttctcgtc cagtccgact ttgatgctga tgtgacgggg
     3181 cgggaagact gggagaggaa gatgaagggg gctctggagc tagccctcca ggtcacagag
     3241 cctaagtcct tcccctggca aggggcagcc aatgttaagt ttccgatcat tacgattgct
     3301 gccctccagt ttcactcccg cgcttacccc gctatggtac cggggccaga cctcgtgcgc
     3361 tgccgtacct tcggcccaga tccgacgggg gagaaggagg ctcgggctac gcgcgtagca
     3421 aaccacatga gctaccagat tctggaggaa gatgagagtt gggaggagga tcatgatcgc
     3481 gtcctcctga cagttcccat catcggctgt gccttcaaga agatctactt caaccaggaa
     3541 ctcggccaca acgtcagtga gtatgttccg gccaaggata tctatatccc ctatttcgca
     3601 aagagtgtgg aggccgctgc tcgctggact catgtagttg agacttcgaa gaatgaactg
     3661 atctcttatc agcgcagagg gtttttctgc gaacaggaac ccctcagcgc tccggtcgat
     3721 ccgcttaagc cctccatcgg ccagccggtc gaacggatgc tgggaacaga gatttccccc
     3781 cgtcaggagg atttgcctta cgaactcctg gagcagcatc gctacctcga tctggatggg
     3841 gacggatacg aagagcctta catcgttact atgattcgag gtagcggtca gctctgccgc
     3901 atcgtcgcgc gctacaccag ccaggatgtg aagagaaact cggcggggga aatctactct
     3961 atcacctgcc agcagaacct tcaaaagtac tcttgcatcc ccagtccgga tggcggtatt
     4021 tatgacctgg ggtttgggat tctcctccag cctctgaatg attcgatcaa tacgatcatc
     4081 aatcagcttc tcgatgcagg aactctggcg aacactgcgg ggggttttct tgggcgcggg
     4141 gcgaagatca gatcggggga tacagccttc tcccccttcg agtggaaacg ggttgatagc
     4201 ctgggggacg accttagaaa gagcatcatt ccccttgatg tgcgggaacc ttctgcagtc
     4261 cttttccagc ttctcggcct tcttattgac tactccgaga gggtagcggg gtcgatggaa
     4321 tcccaggtcg gcatatctcc aggccagaat acccccgctg aaaccactcg gactgttgtg
     4381 gcggaagggc agaaggtttt tatcggaatc ttcaaacgcc tctacagggc aatgaaggga
     4441 gagtttcgga agctgtacaa tctcaatcag gtctatcttc aggaaggcac gaccgtctat
     4501 tatgatgcct cgggagagcg gcagaaaatc ctcagcctcg attacctccc tgacgccaag
     4561 actgtctgcc caatggccga tcccaacatg gttacggata tgcagcggat ccagcaggcc
     4621 caattcctta agcaatccgc catgacgacc gcgggctatg acctccaggc cgtcgagcgg
     4681 cgtttcctag aagccttgaa gatctccgac attcgcacag tctatcctgg acccgataaa
     4741 gtcccgccac ttccgaacgc caaactggaa atcgagaaga tgaaggtcca ggagcgctcc
     4801 caggcccagc ggatggaaat gcaaatggct gccctccagc tcctcgggga agccgaactc
     4861 aaccaggcca gggtgcaaga gcttcgcgcc aaggctgttc tccatctgtc ccaagcagat
     4921 ggggtgaaat cggggcacga aattgccctg ctcgaagctc aaatcggagc cgcgaaggcc
     4981 catcaggaaa gcttgctgaa atcagcacag ctcattttag acagtcttca caaatcagaa
     5041 ggaagagtaa atgatgcaag gcagagcagg ggaactcccc gccccgacgg cggaggaaat
     5101 ccaggaatgg ctgaaccacc cagtaaccaa gtacttcaag gacttcttgg gggacaaggc
     5161 gagggagatg caggcggcct gggctgatgg agtctatacg gatcccacgc agagtacagt
     5221 ggcaagttac gtttgccgtt tgctgcagga catgtccgag atgactctgg atttctttaa
     5281 ctaaaggaag aggtatgaac acttcaggta tccaaccggc tggccacgtt atcctggtca
     5341 agccgaaggt tgtggaagag acaacgaaga gcggcattgt gctggcgacg gcgacgcagc
     5401 ggtcgaggga acacatgggc cagaccgagg caacggtcat cgccctaggc cctaccgcct
     5461 actccgatca ggcccagcca tggtgtgctg taggggatca ggttatcttc gcccgttacg
     5521 ctggtacgga gcgggtaggg gctgacgggg aagtgtaccg gctcatcaac gatctggatg
     5581 tgaaggcaat ctatacggga ggggacaatg tctgatcttg aaacccctaa catgcctgac
     5641 gccccagatc ctgcagaagc tgaagcccgg ctgatcggct ggcggccgcg cgaggaatac
     5701 acaggcccgg aagagcgctg gaagacggcg gaagaatttc tggaggaagg caagcgcatc
     5761 aatggctttc ttcggaagga cttggacaag cttcgcagtg agctggctcg gcgggatgcc
     5821 tcccttgcag agatgaaaca ggccatccag gaattctctg ctcatcacga gaaaacagtc
     5881 aaggccgaat acgaacgcgc cgtgagagac cttcgggcag ctcgcaaaca ggctcttcgg
     5941 gaaaacgacg gggagactgt ggtgcagctt gaggagcagc tggaagccct tggcccacca
     6001 cctacgccga gacgtcccac gcagcctgcc cccaatctcg cccaggaccc tgtgtgggta
     6061 gactggcttg gggaaaacga ctggtttcag aaggacccca aacggagggg gctggcgaat
     6121 gggtacagcg aaatccttcg cgctgagcag cctgagctgg tcggtcggcc ttttctggat
     6181 gaagtcactc ggcgggtgca ggctgatttt cctgaactct tcggtcaggc tcggtctcgc
     6241 ccgcagcagg ttctcactgg tggtcgaggc tcggcagctc cgtctggccg caagtcctac
     6301 gccgacctcc ctgctgaagc cagggaagct tgcgacaagt tcgttcgcct ggggcttgtg
     6361 aagtccaaag aagattacgt aaaagactat ttcggagtag catgatggaa acagttagac
     6421 caacggtcca aaagccgact gagcaagttc gcaatgaagc agagcgggtc cgtcgcccgg
     6481 agcgcattcc ctttggggtg ccgcagtccc ggttgaatgt ccccctgcaa atccctggat
     6541 accatcttca cttcatcaat gactcgccag gtcgcttgca gcaagcccaa ctcggcggct
     6601 atacctttgt agctccttcc gaaatcggct tgtctggcgc cgagacccag tacaaagttc
     6661 tggccgggcg caatgaagat ggatcggcca tgtacgccta tttgatgaaa atcgagctgg
     6721 agttttacga ggaagaccag agatttcacc agaaaacagt agaccagttc gacgaagcca
     6781 tccggcgcgg caagatagaa gaacgtggca acgaaaatcg ctacggcaaa atgaactctc
     6841 aaacttttta aaggagccac taaatggcaa atgcaaatac tccgatcggg ctgaagcccg
     6901 tcaggaccaa taccgccgca ggctgggatg gctcggcgaa cttgtactac atcccctcga
     6961 ctgatacgaa tgcctatcgt atcggggacg cggttgtttc tgtggctggt ggggatactc
     7021 ttacaggtgc tcctgcagtc accctggccg gcactcgcaa tgctgcgatg actacaggaa
     7081 gtctgcgtgg agctatcatt ggtatcggta ctgctgcagg caccgccggc atggccgttc
     7141 ccctggccgc tgaccccaat gccctcggca tcgtcagtat tcccgcaacg aagacgcaaa
     7201 actacttcgt gtgggttacg gacgatccgg gacaagtgta cgagatccaa gccgattcga
     7261 tcgccagcac agctttcaac aagaactgtc cgctgttcgt ggccaatgat ccttcggtgc
     7321 cgacgttcaa ctccgcctcg tatgctcagg gttctcaggc tgataccacg caggccctgc
     7381 cgctcaagat cctcggcgcg ccgttccgcc cggataatga tctgacctcg ccgggcacat
     7441 acgctcgcat cttcgtcatg atcaaccagc atgagctggc cggcaacacg gctggtgtct
     7501 aacccactaa caacctactc aaggagattc aaaaatggct ggtccaatca tgacttccaa
     7561 tcatcccagg tttctgtggc ctggggtgaa gggaatttgg ggtcgtactt acgacgatca
     7621 tcagacggag tacactgatc tgtttgacat cgaaacctcg gacaaggcgt atgaggagtt
     7681 cgtccagatc acgggcttcg gtcttgcgcc ggtgaaggcc cagggcgcgg atgctgtcta
     7741 cgactctgaa gttcagggtc cgatttcccg ctttgttcac ctggcttatg ctctgggcta
     7801 catcgtcacg cacgaagaga ttcaggacaa cttgtatctg gaagtcagca agactcgtgc
     7861 ctccagcaat gctcgtgcct tccggcagac caaggagcgg gtggcagcga atatctacaa
     7921 tcgggccttc agctcgtcgg cagcttactt tggcgccgat ggtgtggccc tgtgttcgac
     7981 ggcccatccc aacaccagtg gcggtacgta ccagaacaag ccgacgatcg acgccgacct
     8041 cagtgaagct tccctggaag acctcctcat cctgatgatg caagctaccg acgaccgtgg
     8101 tctgctcatc aatctgatgc cgaagagcct gcacatcgcg ccgcagaact ggttcaacgc
     8161 caatcgcatt ttgcactcgg tttatcaggc gggcaacgcg aacaatgaca tcaacgttct
     8221 gcgggcaact ggcgccttcc ccggcggtat caagctcaac cattacttca ccgcaccgca
     8281 agcctggttc gttcgttcca atatcgagcg gggcaagggc atggtcttcc tccagcgcga
     8341 gcccatttcg ttcgaccagg acaaggactt cgacaccaag aacgccaagt cactgggcta
     8401 cgagcggtac agctgcggct tcgtcgatcc gcgggcagtc tatggggtta atggtccgta
     8461 aggggtaatt gtgtgtccgt atatccttac cgttatacgg ccatgcaacc ccatgcaatc
     8521 agcaaaactt tacgtggcct accagtcacg ttacctgtaa cgtaaaggag cagcacaatg
     8581 ggaactccca ctcgctttaa gaatggtgtc accaacgccg ccgtcaattc catctttgga
     8641 aacctgccca tccccgatgt taccatcaac gggctgacgg aatacttcaa cgactttcac
     8701 acctacgccg caggagattg gacggtgaca gcctcgggct ctggctcgtc cgccatctcc
     8761 gcaggtttgg gcggtcgtct cgcactgacc tggggcacat ctggcactca aggcaatact
     8821 ctgacttctg gagggtttag ctttaatgcg gcttcgacct ctgccaatgg tagccagttt
     8881 tggttctcct caggtatcat caatcccgcc aatgcctctg cccccaactt tcttgtcggt
     8941 gcgatcaagg gcaatcctac ttccatttct gatggcgtgt actttacaaa ggctgccgct
     9001 ggattgcctt ggcgaatcaa catcaaggcg gctgcagcct cgaccacaac tgttaccctc
     9061 ccgacttcaa ctaacgcagc gaataactcg gtgcaggaac tcggctacta ctatgatggc
     9121 agggcgaatc caaccctgtt catctatgtg aacgatgtga tggtaggaac tttcggagcg
     9181 aacggcagcc ttggcactct ggccaacctc cctgcttcga ccatccttct caacccctcc
     9241 gccgccatcg ggacagctgc tgggcctctc ctggtcgact acctgttctg tgcttgcgaa
     9301 atcccaggcc gcctgtaatc tccagaggct tcggcctctt ttccgaaagg tactattatg
     9361 gataaactaa ttcacagtga agggcgcacg gagcaacgtg ggcgggcaga ggacggcaag
     9421 ccagggcttc gagagaagtg gaaggtctgg ccagggtcgg agttgcctgg agtgaagaag
     9481 aagcgggctg agcctgttca aaaggagccg aaaccatgaa cgaagtccat gtgtgtaatg
     9541 gctttgggta tcgtatgcca gtatcaggaa cagctcttac taccagtgct cctgcaacgg
     9601 gtaatgcgat ctttaaagcc tctcccggtt ctaccttcca gctaatcgcg gctgctgccg
     9661 ccactgcaat cattgaagtg acgaacgagg atgctacggc ggcggggacg aattccaact
     9721 ggatcactat gggcaccatt accctggctg gggcaggtac tgacggcttc atgacccagg
     9781 cctgctggaa gtgggttcgt gccaggatca cagcggcgtc tgcagcaact tccgttttga
     9841 tggggtctta atatgaccat ttctcttgta gataaagttg cagggacaac cccagctggc
     9901 ggcggtggtg ctgtctccag tgtcaattcc cgaaccggag atgttgtttt gactgcggcg
     9961 gatgttgggg ctaccttgct aacaggctct aataccggag acgagacgac tacttccatt
    10021 ggcacgttga tttctggagc aacagaaaaa ccgacgccaa tcgacgcgga cgaactgagc
    10081 at
//
LOCUS       DAOQLC010000140         9899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2661_length_9899_cov_26.093864, whole genome shotgun sequence.
ACCESSION   DAOQLC010000140 DAOQLC010000000
VERSION     DAOQLC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2661_length_9899_cov_26.093864"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..281)
                     /locus_tag="PL000_18105"
     CDS             complement(27..281)
                     /locus_tag="PL000_18105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90850.1"
                     /translation="MKTIYVFGLAFAFLLLVFVFTSVNFNPSLVQAQNQANSFTSLQT
                     TPTPPVEGESVIGSTDGIMLMGVVITVIVVVPLLFHRKKH"
     gene            617..1171
                     /locus_tag="PL000_18110"
     CDS             617..1171
                     /locus_tag="PL000_18110"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNC90851.1"
                     /translation="MIKRIFDWLKQPLGLIAVGIAFLALFSVAAYGLYLTQQPPAQPI
                     QFPHQTHVAFGIQCLYCHPNAAKGPTAGIPAQAKCWACHQQIAKTQTSALLAPLKDAV
                     ISGTPIEWVPVAIVPDFVQFNHRAHIAAGKNCEECHGDMSKVTIAQNPQVMNMGWCLD
                     CHVKNAGEDQVKLVKLTDCGTCHY"
     gene            1192..3369
                     /locus_tag="PL000_18115"
     CDS             1192..3369
                     /locus_tag="PL000_18115"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC90852.1"
                     /translation="MSQQFSRRDFLKLAGVGAATTAILTGCGPASRYVKREPYMQMPE
                     YNYNGQSTYYATTCRECAAGCGLIVRTMQGRALKTEGNANNPLNLGKTCARGQATLHG
                     LYNPDRVTDPMKGGSTITWDEATQTVADALKNNKPEEIAFLMGMAPDHLFDLVTDLAN
                     ATGINAPVRFGALGMFEARATLSKAAENLFGEAKMPFFDVGGAEVVLSFGANYLETWL
                     SPVSYTRGFVGLREAQTKQRGVFIQLEARMSSTAAKADEWLPIRPGTEALVALAIGRL
                     ASELRGGPMPRAFSSVDPKDAAKKSGIKLETLEHIASLFTGAKVALAIPGGSALGQSN
                     GLTVAEAVLALNAVADNFGKPGGVYFSPLAPNQTSYQRPASAKEMQEFIQKMADGKFK
                     VLFVHGVNPVFELPAALGFKDALKGVGQVISFATFPDETASEAGFIFPDHHGLESWGY
                     QRVATGSNQPVLSGSQPVVSPFYNTRATADVLIAAAQLAGGAFATALPFKDEVEFLQS
                     KVAPLMSEADGFFSAADIDTFMATFQQYGGWWKLSDGRGVPGTADVLNRNMDGAEAEF
                     VGEGEFFFAPFVSPTLAEAGANKPWLQELPDPTTTVMWNTWIEVNPETAHELGLHNDD
                     VVKVISEFGELQAYVYLYPAIRPDTIAIPFGQGHTAYGRYAENRGANPANLLGVHFNE
                     AGDFAFSGTKVKVEKVSSAVSGVGLSRMESRIGVYGEALGEEH"
     gene            3372..4097
                     /locus_tag="PL000_18120"
     CDS             3372..4097
                     /locus_tag="PL000_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC90853.1"
                     /translation="MIKLDEVKMSAEENGKWGMVIDQDICTGCQACVAACAMENNISF
                     VGEEDSGYGRSMHWIRIERFWEGEYPEVKMTPQQPMMCQQCGHATCEPVCPAFATVHS
                     QAEQLNLQVYNRCVGTRYCANNCPYQVRAFNWRDYQRPEPLSNQLNPDVTVRRMGVME
                     KCTFCIQRIHKAQDKAKAEGREVVDGEFTTACAQACPANAIVFGRVDNPESLVSQLAH
                     RGHGAKHLEELGTLPNVTYFKGG"
     gene            4100..5527
                     /locus_tag="PL000_18125"
     CDS             4100..5527
                     /locus_tag="PL000_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90854.1"
                     /translation="MSDTKHHEAHSHEEHLREKHLMLDPLPRGKMNDMVMESMLEKPT
                     WKYWVIFAFLSFVVVYFLFYKWGVLIAKGQGQAGVMRPDYWGIFLVNTVFWIGISHAG
                     TFISAILRVFRAEFRRPFTRAAELMTTFGLVQAGFSIFMHMGRVWLFYWLMPYPNQRL
                     LWPNLHSPLTWDLLAITTYLLSSTMYLFLPLIPDVAMARDRSTGWRKNLYKVLALGFR
                     GTEGEWTHLRNAMNIFAWAIIPVMFSVHTIVSWDFAAATRPGWSSTIFGPYFIVGALH
                     SGMGAAAVVLAVIRGTMKNMKYFVRGEHFDAIGKLMLIISMAWAYFFFNDYMVQWYGG
                     DKWTQQLLHFHEAGPLGWMWFGMLFCNIVVPWAILWNAKWRATPWAITIVGLVINVGM
                     WFERYIIVPISVTINRMPFTWRMYTPGIEVPMGIGTVALFILLYMAASKLIPLIPVWE
                     VQEGQMAHELKKFGRETVVAVSELE"
     gene            5537..6073
                     /locus_tag="PL000_18130"
     CDS             5537..6073
                     /locus_tag="PL000_18130"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HNC90855.1"
                     /translation="MSESKIVDLLAVFPDLEPAANAIEQLRTLGVHDDCMNVISGVPV
                     TEAMLGRPHQWTNVPRIAMGGAILGFSGGFFLAFISPYIYPYPIQVSTQAFVPGPPSV
                     VVLFELTMLGMLLSTFLGVFLDSFFPNYRPMKYVPEISDGKIAILVECPHADEKKITD
                     ALNKMGAESVKPAEAQHL"
     gene            6070..6627
                     /locus_tag="PL000_18135"
     CDS             6070..6627
                     /locus_tag="PL000_18135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC90856.1"
                     /translation="MRLFKQLVVVFAVLAVLVGALMVFSYDVIKIDWIVFMEIQPSYS
                     AQENPLPVPAQSIPVEGAAYIPNLGAPSNPVPADETSIARGAELYTIHCLMCHGATGQ
                     GNGTISAFLVKKKPADLTSELVQSKTDGSLFLTISNGIFNPNNTLFPDTEFSGQMPPL
                     SENLSVRERWDLVNYIRTLTVPAAQ"
     gene            6640..7290
                     /locus_tag="PL000_18140"
     CDS             6640..7290
                     /locus_tag="PL000_18140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90857.1"
                     /translation="MKDDVRKYIYGAVTVFLFGVLAWVAIVYVNSCGFTLTCIRGAQP
                     VVRTPIPTLLPATMPAAQTGDGKVVVSDKCRVAAVDLIGAWVEAGSSETEAFQFTDIN
                     GLNCESSFAEVMPLFVESNFWVSGSLSCVSCHSTDVTISLAQLDLSSYAGITSGSRRA
                     DAESKGTDILGGGKWEGSLLYDFLTTAKADVPGHTEAVSDLVIFAGTPAPLATPTP"
     gene            complement(7401..8219)
                     /locus_tag="PL000_18145"
     CDS             complement(7401..8219)
                     /locus_tag="PL000_18145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC90858.1"
                     /translation="MNKLQKFINYDFRDEKHSKWIFIIPSVVLLAFFALPLAAIILRS
                     LNLNFFENALSQQAFKALRLSLLTSSITTLCTAVFGTPLAYILARWKFTHKTWLELIL
                     ELPIVLPPSVAGVALLITFGRNGLLGPFFKFFGISLPFTTTAVVLAQLFVAAPLFLRS
                     ARIGFAHIDQQLEESARVEGANQWQLFSEVTLPLSARALASGAILTWTRALGEFGATI
                     LFAGNLEGMTQTMPMAIYLGFERNIGVALALSVVLIFVSVILLMLTKRLEKNKE"
     gene            complement(8234..9043)
                     /gene="modA"
                     /locus_tag="PL000_18150"
     CDS             complement(8234..9043)
                     /gene="modA"
                     /locus_tag="PL000_18150"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC90859.1"
                     /translation="MKQIFVLTVSLSILAAACNTSIAQISTPTARETIPQTLIVFAAA
                     SLTEAFTEIGAAFDAANPGVNTTFNFAGSQILRTQIEEGAPVDIFASASSKEMESLLT
                     SNLVDSKAPRFFLTNKLVVILPENNPAALEKLEDLANPGIKIILATEEVPVGKYARLS
                     LNLMDASFGSGFKDKVLDNIVSNEDNVRQVVSKVQLGEADAGIVYVSDTMATSELKSI
                     EIPADLNVVAKYPIAPLIKSEHTDLATKFIEYVFSAEGQAILIKWGFTPVK"
     gene            complement(9179..9724)
                     /locus_tag="PL000_18155"
     CDS             complement(9179..9724)
                     /locus_tag="PL000_18155"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNC90860.1"
                     /translation="MTDKKTHHIGTLSPEMALLGLLYKEPGHGYDLHRKVNTDLGQVW
                     HLSQSQAYAILKRLEMQGDISTKKISQGKLPSRQLLQMTAQGRQHFLNWLHTASGGST
                     RVIRMEFITRLYFLKMYLPQKITKAFEQQRAEINTHIKRLEENLHTLPTDQIYNRMSL
                     EMRVKQLVLTLEWLDENQKNF"
BASE COUNT         2317 a         2495 c         2495 g         2592 t
ORIGIN      
        1 agcagcggga tgtttgtttt gtaggtttag tgtttttttc gatggaagag caacggtaca
       61 acgacaatga ccgtgatgac cactcccatc agcatgatgc catctgtgga accgatgacc
      121 gattcgcctt caactggtgg ggtgggggtt gtttgcagtg acgtgaagga atttgcctga
      181 ttttgcgcct gcacaaggga tgggttgaag tttacgctcg tgaatacaaa aacaagcaaa
      241 agaaaagcaa aggccagccc aaaaacatag atcgttttca ttgttatcgt tgccaattgt
      301 aacacggctc aaaagtctct aatttaactc ttatatgtac ttttctgcac tttcgatgta
      361 taatccgccg agttaatttg ctggcggggt tcagcaaatt tgtatttgga gaatgcatga
      421 gtaatctgac gaaaattcta cgcccatttc tcgtcctgat gaatatcggg acgggtttta
      481 tgtatatgca tttaatgggg cggcgggttt cggctcgtaa cttttgcatt tgtacaatgt
      541 ttgtatccat gtaaagtagc cttcgccgcc ttcacccggc ggcgttgcgc ttaaaagatt
      601 catttcagga gcatccatga tcaaaagaat ttttgactgg ctgaaacagc ctcttggtct
      661 gatcgcggtg gggatcgctt tcctggcgtt gttcagtgta gcggcttatg gtttgtacct
      721 tacccagcag ccacctgcac aacccattca gttcccccat cagactcacg ttgcatttgg
      781 tattcaatgt ttgtattgtc atccaaatgc ggcgaaggga ccaaccgctg gcatcccagc
      841 ccaggccaaa tgttgggcct gccaccaaca gatcgccaag acacagacga gcgccctgct
      901 tgcaccgtta aaggacgcag tcatcagtgg tacccccatc gagtgggtgc cggttgcgat
      961 cgtgccagac tttgttcagt ttaatcaccg tgcgcatatt gcggctggga agaactgcga
     1021 agaatgtcac ggcgacatga gcaaagtgac cattgcccaa aacccgcagg tgatgaacat
     1081 ggggtggtgt ctggattgtc acgtgaagaa tgccggtgaa gatcaggtca aactggtcaa
     1141 actcaccgac tgcggcactt gtcactatta aaccgggcga aaggaatcaa tatgagccaa
     1201 caattttccc ggcgtgattt tctgaaactg gctggtgtgg gcgcggcaac gactgcgatc
     1261 ctcaccggtt gtgggcctgc ttcacggtat gtcaaacgcg aaccgtacat gcagatgccc
     1321 gaatacaact ataacggtca gagcacctat tacgccacca cttgccgcga atgtgcggcc
     1381 ggatgcggac tcattgtccg caccatgcag ggacgtgccc tcaaaaccga aggcaatgcg
     1441 aacaatccgc tgaacctcgg aaagacttgc gcacgcggac aggccacatt acacggcttg
     1501 tacaaccccg atcgcgtaac cgacccaatg aagggcggta gcacgatcac atgggatgaa
     1561 gcaactcaga cggttgcaga tgctctcaag aacaacaagc ccgaagagat cgccttcctg
     1621 atgggaatgg cccccgatca tctctttgac ctggtaactg acctcgccaa tgcaacaggc
     1681 atcaatgccc cggtccgctt tggtgctttg ggtatgttcg aagcccgtgc cactttgagc
     1741 aaggccgctg agaatctttt cggtgaagcc aagatgccct tctttgatgt gggtggggcg
     1801 gaagtggtcc tctcctttgg tgcaaactac ctcgaaacat ggctttctcc tgtttcatat
     1861 actcgtggct ttgtcggcct gcgtgaagcc caaaccaagc agcgcggagt cttcatccaa
     1921 ctcgaagcgc gtatgtcttc caccgccgcc aaggcggatg aatggctccc gattcgcccc
     1981 gggaccgagg cactggttgc gcttgctatc ggcagactcg cctctgaatt gcgtggcggc
     2041 cccatgcccc gcgctttctc aagtgtggat ccgaaagatg ctgccaagaa atcgggcatc
     2101 aaactcgaaa cccttgagca tattgccagc ctgttcaccg gcgcgaaggt ggcgcttgcc
     2161 atccccggtg gttccgctct tggtcagagc aacggcctta cggttgccga agccgtcctt
     2221 gctttgaatg cagttgccga taacttcggc aaacctggtg gtgtgtactt ctcgccgttg
     2281 gccccgaacc agaccagcta tcagcgccct gcttccgcca aggaaatgca ggaattcatc
     2341 caaaagatgg ctgacgggaa attcaaggtg ttgttcgtgc atggtgtcaa ccctgtattc
     2401 gaacttcccg ccgctttagg tttcaaggat gcattaaaag gtgtgggtca ggtgatttcc
     2461 tttgcgacct tccccgatga aaccgcaagt gaagctggtt tcattttccc cgatcaccac
     2521 ggacttgaat cctggggata ccagcgtgta gccactggtt ccaaccaacc cgtcctctcc
     2581 ggctcacagc ctgttgtatc accgttctac aacacccgcg ctacagccga tgtcttgatc
     2641 gctgccgccc agctggctgg tggcgcattc gcaactgcct tgccgttcaa agatgaagtt
     2701 gaattcttgc agagcaaagt tgccccgctg atgagcgaag ccgatggctt cttctccgca
     2761 gccgatattg atactttcat ggctaccttc cagcaatatg gtggatggtg gaaactttct
     2821 gatggccgtg gcgtgcccgg cacagcagac gttctcaacc gcaacatgga tggtgccgaa
     2881 gctgaatttg ttggtgaagg tgagttcttc ttcgccccat tcgtttcacc gacccttgcc
     2941 gaagcaggtg caaacaaacc ctggcttcag gaattgcccg acccaaccac gactgttatg
     3001 tggaatacct ggattgaagt aaaccctgaa actgctcatg aactgggcct gcataatgat
     3061 gatgtcgtca aggtgatcag tgagtttggt gagttacagg cgtatgttta cctgtaccct
     3121 gcaattcgcc ccgacacgat cgccattccg tttgggcagg gtcacactgc atatgggcgt
     3181 tatgctgaaa atcgcggcgc caatcctgca aatttgcttg gcgttcactt taacgaagcc
     3241 ggtgattttg catttagcgg tacaaaggtc aaagttgaaa aggtctccag tgctgtaagc
     3301 ggtgttggtc tgtctcgcat ggaaagccgc attggtgtgt atggcgaagc gctgggtgag
     3361 gagcattaat tatgattaaa cttgatgaag tcaaaatgag cgcggaagaa aacggcaaat
     3421 ggggcatggt cattgaccag gacatttgca cgggttgtca ggcctgcgtt gccgcctgtg
     3481 ccatggagaa taacatctcc tttgtcggcg aagaagattc aggctatggc cgttccatgc
     3541 actggattcg cattgaacgc ttctgggaag gggaatatcc tgaagtgaag atgacccccc
     3601 aacagcccat gatgtgccag caatgcgggc atgccacttg tgaaccggtc tgcccggcat
     3661 ttgccactgt tcactcacag gctgagcaat tgaaccttca agtgtataac cgctgtgtgg
     3721 gtacgcgtta ttgcgcgaac aactgcccgt accaggtgcg tgcattcaac tggcgcgact
     3781 atcagcgccc tgagccgctt tccaaccaac tcaaccctga tgttacagta cgccgcatgg
     3841 gtgtaatgga aaaatgcacc ttctgcatcc agcgcattca caaagcacag gacaaggcca
     3901 aagcggaggg acgcgaggtt gtggatggtg aattcaccac cgcgtgtgca caggcctgcc
     3961 ctgcgaatgc gatcgttttt gggcgcgtag ataaccccga atcgctggtt tctcaactgg
     4021 ctcatcgtgg tcatggcgcc aaacatcttg aagaactcgg cacgcttccc aacgtgacct
     4081 acttcaaggg agggtaaaca tgtcagatac aaaacatcac gaggcacact cgcacgaaga
     4141 acatcttcgc gaaaaacacc tcatgctcga cccgctgcca cgcggaaaga tgaacgacat
     4201 ggtcatggaa tccatgttgg aaaaacccac ctggaaatac tgggtgatct ttgcctttct
     4261 ctcttttgtg gtagtgtatt tcctcttcta taagtggggt gtcctgatcg caaaaggtca
     4321 gggtcaagcc ggtgttatgc gccctgacta ctggggtatt ttcctcgtca acaccgtgtt
     4381 ttggatcggt atcagccatg caggaacctt catttctgcg attttgcgcg ttttccgcgc
     4441 tgaattccgc cgtccattca cccgtgccgc agaattgatg accacctttg gcctggtaca
     4501 ggctgggttt tccatcttca tgcacatggg tcgtgtgtgg ctcttctact ggcttatgcc
     4561 ttaccccaat cagcgcttgt tgtggcccaa cctgcattct ccactcacat gggacttgct
     4621 ggccatcacc acctatctgc tttcctccac catgtatttg ttcctgccac tcattccaga
     4681 tgtggccatg gctcgcgatc gctccactgg ctggcgcaag aatctgtaca aggttctcgc
     4741 acttggcttc cgtggtacag aaggtgaatg gacccatctg cgcaacgcaa tgaacatctt
     4801 tgcatgggcg atcatcccgg tcatgttctc agttcacacc atcgtgtcct gggactttgc
     4861 agcagccacc cgcccaggtt ggtcttcgac catctttggc ccgtacttca tcgtcggcgc
     4921 gttgcattcc ggtatgggcg cggcagccgt cgtgttggca gtgatccgcg gcaccatgaa
     4981 gaatatgaaa tacttcgtgc gcggtgaaca cttcgatgcc atcggcaaac tcatgctcat
     5041 catctccatg gcttgggctt acttcttctt caatgattac atggttcaat ggtacggcgg
     5101 cgataagtgg acacagcaat tgctgcactt ccatgaggct gggccgttgg gctggatgtg
     5161 gtttggcatg ctgttctgta acattgttgt tccctgggct attttgtgga acgcaaagtg
     5221 gcgcgccaca ccatgggcca tcaccatcgt tgggttggtc atcaatgtcg gtatgtggtt
     5281 tgaacgctac atcatcgttc cgatctcggt aaccatcaac cgtatgccct tcacctggcg
     5341 catgtacacc ccgggcattg aagtccccat ggggatcggc acagtggctt tgttcatcct
     5401 gctttacatg gcggcgtcca agttgattcc gcttatcccg gtttgggaag tgcaggaagg
     5461 tcagatggca cacgaactca agaaatttgg acgcgagacc gtggttgcgg tcagcgaatt
     5521 ggaataggag gacgacatgt ctgaatcgaa aattgtcgac ctgcttgcgg tcttccccga
     5581 cctcgaacct gctgccaatg ccattgagca gcttcgcacc ttgggcgttc acgatgattg
     5641 catgaacgtc atctctggtg tgcccgttac tgaagccatg cttggccgtc ctcaccagtg
     5701 gacaaacgtg ccgcgcattg ccatgggcgg tgccattttg ggcttttctg gaggcttctt
     5761 tttggctttc atctcgccct acatttaccc ataccccatt caagtcagca cacaagcatt
     5821 cgtccctggt cccccgtctg tggtggtgct ctttgagttg accatgctgg gtatgttgct
     5881 ttcaactttc ctcggcgtat ttctcgatag tttcttcccc aactaccgcc cgatgaaata
     5941 cgtgcccgag atcagcgacg gcaagatcgc cattttggtg gaatgtccac atgctgatga
     6001 aaagaaaatt acggacgcct taaacaaaat gggcgctgaa tccgtgaaac ctgcggaggc
     6061 gcaacaccta tgagactctt taagcaactc gttgtcgtat ttgcggtcct tgccgtgctg
     6121 gttggcgcgt tgatggtctt ctcctacgac gtgatcaaaa ttgactggat cgtcttcatg
     6181 gagatccaac cctcctacag cgcgcaggaa aacccgctgc cggtgcctgc tcaatccatt
     6241 ccggtggaag gcgccgccta cattcctaat ctgggcgccc catccaatcc tgttcctgct
     6301 gatgaaacct ccattgcgcg tggtgccgaa ctgtacacca ttcactgcct gatgtgccat
     6361 ggagccactg ggcagggcaa tggcaccatc tctgcttttc tggtgaagaa gaaacccgct
     6421 gatttgacca gtgaacttgt ccagagcaag accgatggca gtctgttcct gaccatttcc
     6481 aatggtatct tcaatcccaa taacaccctc ttccctgata ctgaattctc cgggcaaatg
     6541 ccgcctttga gcgagaatct ctcggtacgc gaacgctggg atttggtcaa ttacatccgc
     6601 acgcttaccg tgcccgcggc gcagtaggga ggtacccata tgaaagatga tgttagaaag
     6661 tatatctacg gcgcagtcac tgtgttcctg tttggcgtgc tggcctgggt tgccattgtg
     6721 tatgtcaact cttgcgggtt cacgttgact tgtatccgcg gtgcgcagcc tgtggtacga
     6781 accccgatcc cgaccctgct gcctgccacc atgcctgccg cacaaaccgg tgatggaaaa
     6841 gtggtcgttt ctgataaatg ccgtgtagct gctgtggatt tgatcggcgc atgggttgaa
     6901 gctggctcct ctgaaaccga ggccttccaa ttcaccgata tcaacggctt gaactgtgaa
     6961 tcttcttttg cggaagtgat gccgctcttt gtggaatcca atttctgggt ttcagggtcg
     7021 ctctcctgcg tttcctgcca ctcgacagat gtgaccatct ctctcgccca gcttgacctg
     7081 agcagttatg caggcattac ctctggctcg cgccgtgccg atgccgaatc caaaggcact
     7141 gacatccttg gcggtggaaa atgggaaggc tcattgcttt acgacttcct gaccacagcc
     7201 aaagctgatg ttcccggaca cactgaagcg gtttcagatc tggtaatctt cgcaggtaca
     7261 cctgctccgc ttgcaacccc aacaccctaa aaaattgagt ttagtgacaa aaacaccccg
     7321 aaacattcgt ttcggggtgt ttttgcttta atgtcaatgg agccctgatt gcaaaactta
     7381 tatgaatttt ttgctaagtt ttattccttg tttttttcca accttttagt aagcatgagc
     7441 aatatgaccg atacaaagat caacacaact gagagtgcca atgctacacc gatgttccgc
     7501 tcaaaaccaa gatagatggc cattggcatg gtctgggtca taccctccaa attccctgcg
     7561 aatagaattg ttgccccgaa ttcacccaga gcgcgtgtcc atgtcaggat ggccccactt
     7621 gcaagcgctc gagcagataa tggtagcgtg acctctgaaa atagttgcca ttgatttgct
     7681 ccttccactc gcgcagattc ttcaagctgt tggtcaatgt gtgcaaaacc gattcgtgct
     7741 gaacggagga ataatggtgc cgccacgaac aattgtgcca gcaccacagc agttgttgta
     7801 aatggcaggc tgatcccaaa aaacttaaaa aacggaccga gtaatccgtt tcgtccgaaa
     7861 gtgatcaata acgccactcc tgcaaccgag ggagggagca caatggggag ttcaagaatt
     7921 aactccagcc acgttttgtg tgtaaatttc caacgtgcca gaatataggc gaggggtgtg
     7981 ccgaaaactg ctgtacataa ggtggtgatt gaactggtta gcaggcttag ccgcagcgcc
     8041 ttgaaagctt gttgcgataa ggcattttca aagaaattca gattgagcga acgtagaatg
     8101 atcgcagcca gcggaagtgc aaaaaaagca agcaaaacca cactggggat gatgaatatc
     8161 cattttgagt gtttttcatc ccggaaatcg taattaataa atttttgtaa cttattcata
     8221 tgaagaccga atctcatttg actggggtaa atccccactt gatgaggatc gcctgccctt
     8281 ctgctgaaaa tacatactcg atgaattttg tagctaaatc tgtatgttct gatttaatca
     8341 atggcgcaat gggatacttt gcgacgacat tcaagtcagc gggaatttca atggatttca
     8401 attcggaagt tgccatggtg tcagagacat agacaatacc tgcatctgct tcgccaagct
     8461 ggactttgga taccacctgc ctgacattgt cttcgttgga aacgatattg tccaaaactt
     8521 tgtccttgaa acctgatcca aatgaagcat ccatcaagtt gagggataga cgtgcgtact
     8581 tgccgacagg tacttcttct gtagctaaaa taattttgat gcctgggttt gcgaggtctt
     8641 ccaatttttc gagggcagca gggttgtttt ctggcaggat gacaactaat ttatttgtca
     8701 gaaaaaaacg tggcgccttt gaatcgacaa gattacttgt gagaagggat tccatttctt
     8761 tgctgctggc agaagcaaat atatcaacag gtgcaccttc ttcgatttga gttcgcagga
     8821 tttgcgagcc tgcaaagttg aatgttgtgt tgacgccagg attagcggca tcaaacgcag
     8881 cgccgatctc ggtaaatgcc tcagttaagg aggccgcagc aaaaacgatc aaagtttgtg
     8941 gaattgtctc cctggcagtg ggagtagata tttgtgcgat agaggtatta caggccgcag
     9001 ccaagatgga caaagaaaca gttaggacga atatttgttt catcaaactc tccgaaatcg
     9061 ggaaaacagt tttatactca ctgagtgagt atagcgtaga atcttcgaac gatacaaata
     9121 cttttactga aacatgagat cggttttatg aattattccc cgtttatttc atgggaacct
     9181 agaagttctt ctgattctcg tccagccatt ccagtgtgag aaccaattgc tttacacgca
     9241 tctccaaact catacggtta tagatttggt cggttggcaa tgtgtgcaaa ttttcttcca
     9301 gacgtttaat gtgtgtattg atttcagcgc gttgctgctc gaaagctttt gtaatttttt
     9361 gtgggagata catttttaga aaatacaagc gggtaataaa ttccatgcgg atgacacggg
     9421 tgcttccgcc actggcagta tgaagccagt ttaagaaatg ttgtcgccct tgtgcagtca
     9481 tttgcaacag ctgacggctg gggagttttc cctgtgagat ttttttggtc gaaatatcgc
     9541 cttgcatttc caaccgtttt aaaatggcgt atgcctgact ttgacttaag tgccagacct
     9601 gcccaaggtc tgtattgacc ttacgatgca ggtcgtagcc atgaccgggt tccttgtata
     9661 gcaggccaag taaagccatt tcaggtgata gtgttccgat gtgatgtgtt tttttgtctg
     9721 tcatatactc accaaacaag tagcctgatt tgatgagtat atcgtaaaaa caggagtaac
     9781 tttttgaaat aaattcctgc catggttgga gattgtttgc attttaagac tttatattta
     9841 atacgttttt ctggcggaga gggcgggatt cgaatacaac ccctcgatgg ggcgtgcgg
//
LOCUS       DAOQLC010000141         9864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2683_length_9864_cov_23.456927, whole genome shotgun sequence.
ACCESSION   DAOQLC010000141 DAOQLC010000000
VERSION     DAOQLC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_9864_cov_23.456927"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PL000_18160"
     CDS             <1..938
                     /locus_tag="PL000_18160"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HNC90861.1"
                     /translation="IGKLRGLQQIASKRGTFTALALDHRQNLRKANPAFVDDTELSRF
                     KLDVTKALAHRATSVLLDPEVSAAQAIAQNVIPSTVGLVVAVESTGYTGDATARHAQI
                     IPGWSVEKAKRMGASAIKLLVYYHPDSPTAQETEAFTKNIADECARHDLVLMLEPLSY
                     SLNDKKLSSDEKRYVVVESAKRLTPLGADILKAEFPLDVAETDQSLWKEACEEISAAT
                     DIPWILLSAAVEYETFLQQVVVACNAGASGIAVGRAVWKEAVTMTSDERVKFLRMVGR
                     QRMSRLTSLCHALAKPFYDFYQAEAPFGWHKTYGE"
     gene            940..2091
                     /locus_tag="PL000_18165"
     CDS             940..2091
                     /locus_tag="PL000_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major facilitator superfamily domain-containing
                     protein 6"
                     /protein_id="HNC90862.1"
                     /translation="MKKIWPFGYYFAFFAGMSAFVPYIVLYYQTLDFSGTQIGLLVAL
                     SSLISLVGGPFMTGLADSGNRHKLIMSLALTVAAVSMFVLPFLRTFLPVLILIGVFSF
                     ISAPANSLADSAAMSMLGDEKHLYGRVRMGGTFGWALTAPLAGIIVARYGLSWAFWAY
                     ALFMVIALLISLNFLYTEQTEANPFWQGVRALLGNRQLMLFMFMAFVCGTAFATVNNY
                     LFAYMNQLHIDESQMGYTLTIATAAEIPVLFFANRLLSQFKPRGLLILSMAATGLRLL
                     LYAFFDSLTGILVFQLLNGLTFPALWVAGVSYSNDNAPPGLSATTQAVFGAMIFGFGA
                     ATGGFLGGILLENLGGQMMYAIFGAVTLVMLVIYVFLEPRFAARHEKAN"
     gene            2075..>2464
                     /locus_tag="PL000_18170"
     CDS             2075..>2464
                     /locus_tag="PL000_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNC90863.1"
                     /translation="MKKPIDILVAGEINPDLILTGDVKPEFDQVEKLVTSATLTVGSS
                     SVIFACGAARLGLRVAFIGVCGDDVFGRFMLAEMQKRNVNVDHVIIRTAGQTGLSVIL
                     NREADRAILTHPGLIADLQASDIADSLL"
     assembly_gap    2465..2564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2565..3576
                     /locus_tag="PL000_18175"
     CDS             <2565..3576
                     /locus_tag="PL000_18175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HNC90864.1"
                     /translation="GLCVRSFIHAKGGRHRRPTHPARSAGLFMILCLTPNPAIDRSMD
                     VDALQIGEVHRAKKTLAAAGGKGINVARAIRTLGGEPLCMGPIGGHAGNLLADLAKGE
                     GFSAHWTRVQNETRTCIILVQPNQDATVVNEVGQSIRAEECDALIHDVWEQSERVGLV
                     SVSGSLPPGFPLERYQALLAGLVERGKSVWVDTSGPALKTALTVSGIHIKVNAAELSE
                     ALGVEISNAEQAAKAMQHLHQRGISQAVITLGRHGAVFSSEAGVWLAQPPQLKIVSSV
                     GSGDAFLGGLLFALENGASSETALQKAVAAGAANTLEFGGGTFSLPSFEEILQNTKIS
                     LM"
     gene            3765..9863
                     /locus_tag="PL000_18180"
     CDS             3765..9863
                     /locus_tag="PL000_18180"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNC90865.1"
                     /translation="MRDHQQNSFEQWRALLIMGAIILAVGGFLFVFQRLQGPTPDSVP
                     VEVDVTESSPGGDDVQDDNYSILVDLSSGQSQPENTNPLPQATGEPLSSAEIEAILAR
                     LPALTADSAAVDFNIPQEILPPPRPGNTVEETFPPFETESGPGEVESGPLQVLRFSPE
                     GEIPIAPFISVTFNQAMIPLGTLSDLAATDVPVTIEPPLPGTWRWLGTKTLTFEYDSD
                     EIDRLPKATEFHVTIPAGTLSINGNALAEAVTWTFKTPPPTVVSFYPEDAPQPLDPVF
                     FLQFDQRIDPAAVLKTIAVTAGDQQIEIVPATQEQIEADERVSALTKDAPEGRWLAFR
                     ATQPFKTETRISVVVGPQVPSLEGPLTTTTTQTFGFSTYSPLRVTGQRCSWYEDRCPP
                     LSPFFITFNNPLDLDSFSEDMLSVSPEIPGVSASVYATDLQISGETRGQTTYTVTVSG
                     KIRDIFGQALGEDQQLKFKVDKADPILVNPEQTLVTLDPSSKEPLYSAYVMNYNKLNL
                     KVYAVQPSDWPQYRTYLREWQQDNSNAKMPGRLVEEKTLNLDFPDDTLSEVEIKLRPY
                     MDGEFGHFVLVIEPPAGIFESDDEQWRRFSQTIVIWVQVTQIGFDVYTDHSEMIAWTT
                     NLKDGAPLTGVKIMPENGGPQTLSDAEGIARFDIPNGATYLTASLGADTVLLPRSGYY
                     WDEGGWSAEAPLDTVQWFVFDDRQMYRPGEEVHLKGWLRRVGGRQGGDVELPGDAVRS
                     VSYQLFEPQGNLIGEGKLDVNSLGGFDLAFTLPEGINLGFAQLYLTAEGDVNGLTNLQ
                     YFHSLAVQEFRRPEFEVTARNETAGPYFAGEQATVAVDAKYYAGGGLPGADVRWNVAS
                     NPGTYSPPNWPDFTFGTQRNWWLFSDFLYQNGSFSQGEYFEGQTDALGTHYLNLNFKP
                     QGDPDKNPQPYTITASATVIDVNRQAWSSGTSLLVHPADVYIGLRTASYFVERGEALN
                     VEYIVTDLDGNPVKGREVVITAGVLDWKFKNGDWVEEVVDVQTCSLLSTPEPGLCSFE
                     TPLGGSYQITALVTDELGRKNQSRILRWVSGGKQPPSRNLEQEEVTLIPDKENYQPGD
                     IAKILVQAPFSPAEGLLTVTRNGILYTTRFTVEDGTATLRIPIEEEHIPNLNIQVDLV
                     GSAPRTGDDGETLENIPPRPAFASGNMTLNIPPEQRTLSLQLTPAQSTLEPGGETNLT
                     VLVKDANGKPVADAELAVVVVDEAILALTNYQMKDPLSIFYTTRSSYLSSYYSRASLI
                     LANPQSLAAQSLSRQAADSVLYAAATEAPASVEMPMSEALTLTGGGGETLPDAAIAVR
                     ANFNPLAAFEPTIRTGSNGEARVFIQLPDNLTRYRVMVVAVDDGGSRFGVGESNLTAR
                     LPLMVRPSAPRFLNFGDTFELPVVLQNQTNEPMMVDVVARATNLALENAGQRVTVPAN
                     DRVEVRFNASTLMAGEVQIQFAAVSGGYADAATANLPVYTPATSEAFATYGVLDEGTI
                     AQPVQYPSGVFPQFGGLEVSTSSTALQALTDAVLYLVQYPYECSEQVASRILAVASLR
                     DVLSAFKAEGLPTPSEMEAAVSVDIDRLQSMQNADGGYPYWRRGFESSPFNTIHVTHA
                     LFRAQEKGFNVPVEMQQNSLSYLRDIESHYPSWYGNETRWTLSAYALFVRNLMGDRDS
                     QKAEALLNEAGLENLPMEAIGWLWSVIDDAEQLDAIRLHVNNRVVETAGAANFTTAYT
                     EQTYLLLSSDRRTDAILLDALIEDDPQSDLIPKLVNGLLAAARQTQGRWGSTQENVFV
                     LTALDRYFNTYESQTPDFVARIWLGDTYAGSNEFRGYTTDVSETLIPMNYVLSETSSG
                     GGMQDLILSKDGPGRLYYRLGLQYAPTELDLDPLDMGFVVERRYEGVDDPEDVTLDAD
                     GIWHVKAGARVKVTVTMAADNRRYHVALVDPLPAGFEIINPSLAVSQPVPQDPTSPDF
                     KYGWWWWGPWYEHQNMRDSRAEAFTSLLWDGVYEYVYYVRATTPGTFITPPAKAEEMY
                     SPEVFGRSGTDRVIVK"
BASE COUNT         2123 a         2668 c         2686 g         2287 t
ORIGIN      
        1 ccatcggcaa actccgcgga ctgcaacaga tcgcctccaa acgcggcacc ttcaccgccc
       61 tcgccctcga ccatcgtcaa aacctgcgca aagccaaccc cgccttcgta gacgataccg
      121 aactctcccg cttcaaactc gatgtgacca aagcactcgc ccatcgtgca acttctgttt
      181 tgctagaccc tgaagtctct gccgcgcaag ctatcgcgca aaacgtcatt cccagcactg
      241 tcggtcttgt ggtcgctgtc gaatcaacgg ggtacacagg cgatgccacc gcccgccacg
      301 cgcagatcat ccctggctgg tctgttgaaa aagccaaacg catgggcgca tcggcgatca
      361 aattgttggt ctactatcac cccgattccc ccactgccca agagaccgaa gcctttacaa
      421 aaaacatcgc cgatgaatgc gccaggcacg atctcgtgct gatgctcgag ccgctatcct
      481 attcgctcaa tgataaaaaa ctatcttctg atgaaaaaag atatgttgtc gtggaaagcg
      541 ccaaacgtct cacaccgctc ggagcagata ttctcaaagc tgaattcccg ctcgatgtcg
      601 cagagacaga tcaatcttta tggaaagaag cctgtgaaga aatctctgcc gcaaccgaca
      661 ttccctggat cctgctctcc gctgcggtcg agtacgaaac cttcctgcag caagttgtcg
      721 ttgcatgtaa cgcaggcgcc agcggaatcg ccgtcggtcg cgccgtatgg aaggaagccg
      781 ttacgatgac cagtgacgaa cgtgtcaaat tcctgcgtat ggttgggcgt cagcgcatgt
      841 cacgcctcac atccttgtgt catgcacttg ccaaaccgtt ctacgatttt tatcaagccg
      901 aagcgccgtt cggttggcac aaaacatatg gagaatagat tgaagaagat ctggccgttt
      961 ggttactact ttgctttttt cgcggggatg tcggcttttg ttccgtacat tgttctttac
     1021 tatcaaaccc ttgatttctc aggcacgcag atcggtttac tggtggcgct ttcgtcgctg
     1081 atctctctgg ttggcggacc cttcatgaca gggctggccg attcggggaa ccgtcacaaa
     1141 ttgatcatgt ctctggcgct gaccgttgca gcagtatcca tgttcgtgct gccttttctg
     1201 cgcacctttc tccccgtgtt gatcttgatc ggcgttttct cttttatatc ggcaccagcc
     1261 aattcactgg ccgatagcgc cgccatgtcg atgctgggag atgaaaaaca tttgtatggc
     1321 cgtgtgcgca tgggcgggac atttggctgg gcgctcacgg ctccgttggc ggggatcatt
     1381 gttgcccgtt acggattatc gtgggcattt tgggcatatg ccctttttat ggtgatcgca
     1441 ttattgatca gcctgaattt cctgtacacc gaacaaacag aagcaaatcc attttggcag
     1501 ggagtgcgtg ccttgctggg aaaccgccag ttgatgttgt tcatgttcat ggcttttgta
     1561 tgcggtactg cctttgcgac ggtcaataat tatttgttcg catacatgaa ccagcttcac
     1621 attgacgaat ctcaaatggg atatacactc accattgcaa cggctgccga gattcctgtg
     1681 ctgttctttg ccaatcgttt gctgagtcaa ttcaaaccgc gcggtttgtt gatcctttca
     1741 atggcggcca caggcctgcg cctgctgttg tacgcgttct tcgattcgct gacgggcatt
     1801 ctggtcttcc agcttctcaa tggacttacg tttcctgcgc tgtgggtggc gggggtctcc
     1861 tattccaacg acaacgcacc accagggtta agtgccacca cccaggctgt attcggcgca
     1921 atgatctttg gcttcggcgc agccacaggc ggattccttg gcggaatcct tttggagaac
     1981 ctcggcggcc agatgatgta cgcgattttc ggcgcagtga cactggtcat gcttgtgatc
     2041 tatgtcttcc tcgaaccacg ctttgccgct cggcatgaaa aagccaattg atattctcgt
     2101 tgctggtgag atcaaccccg acctgatcct gacgggtgat gtaaaacccg aattcgatca
     2161 ggttgaaaag ttggtcactt ctgcaacgct taccgttggt tcgtcctccg tcatctttgc
     2221 ttgtggcgcg gcacggcttg gattgcgggt tgccttcatc ggcgtgtgcg gcgacgatgt
     2281 cttcgggcgc ttcatgctcg ctgaaatgca aaaacggaac gtgaatgtcg atcacgtcat
     2341 catccgaacc gctgggcaga ctggtctcag cgtaattttg aatcgcgaag cggaccgcgc
     2401 gattctgact cacccaggcc tgatcgctga ccttcaagca tccgatatcg ctgacagttt
     2461 gctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggctt gtgtgtgcgg
     2581 agctttatcc acgcaaaggg cgggcggcac cgaaggccaa cccaccctgc aagaagcgct
     2641 ggccttttta tgatcctctg cctgaccccc aaccccgcca ttgaccgctc catggatgtg
     2701 gatgcattgc agatcggtga ggtgcatcgc gcaaaaaaga cactggccgc tgcaggtgga
     2761 aaaggaatca atgttgcacg tgccatccgc acgttgggcg gggaaccgtt gtgcatgggg
     2821 ccgatcggtg ggcatgctgg gaatctgcta gccgacctcg caaagggtga ggggttctcc
     2881 gcgcattgga caagggtgca aaatgaaacc cgcacttgca tcattctcgt acaacccaat
     2941 caggatgcca cggtcgtcaa tgaagtcggg caatccattc gtgcagagga atgtgatgcc
     3001 ctgatccacg atgtttggga gcagtcagag cgggttgggc tggtttctgt gagtggcagc
     3061 ctgccgccag gctttccgct tgaacgctac caagcattgc ttgcagggtt ggtggagcgc
     3121 ggcaaatccg tttgggtgga taccagcgga cccgccctga aaactgcatt gaccgtgtcg
     3181 ggaatccaca tcaaggtcaa tgccgctgaa ttgagcgagg cgcttggcgt ggaaatttcc
     3241 aacgcagagc aggcggctaa agcgatgcag caccttcatc agcggggaat ttcgcaagcg
     3301 gtcatcacgc ttggcaggca tggcgctgtt ttcagttcgg aggcgggggt gtggctcgcg
     3361 cagccacctc aattgaaaat cgtcagcagc gttggcagcg gcgacgcatt tttgggcggg
     3421 cttttgttcg cacttgaaaa tggcgcatcc tctgaaactg ccttgcaaaa agcagttgcc
     3481 gcaggggcgg cgaacacact cgaatttggc gggggaactt tttcattgcc ttcctttgaa
     3541 gagattttac aaaacacaaa aatttccctg atgtaaagca ggatcattcc cattgcattg
     3601 aactcgttat aataacctct cgaaaagtct gaaagattaa cctctgcggg ttcttgccct
     3661 ggcagttcaa aaatacttcc atgtgatttt tgaaggcggc gaagtctttg gcgggaaccg
     3721 aacccctgtt tgcagcgtcg tgatacggga aggaagaata tgccatgaga gatcaccagc
     3781 aaaactcgtt cgaacaatgg cgcgccctgt tgatcatggg tgccattatc ctggctgtgg
     3841 gcggtttcct gtttgtgttt caacgtttgc aggggccaac gccagattct gtccctgttg
     3901 aagttgatgt aaccgaatcg tccccgggag gtgatgacgt gcaagacgat aattacagca
     3961 ttcttgtgga tttgagcagc ggtcaatcgc agcctgagaa tacgaatcca ttgccgcagg
     4021 cgacgggcga accgctttcc tctgcggaga tcgaggcgat tctcgcgcgt ctccctgcgc
     4081 tgacagctga ttctgccgcg gtggacttta acatcccgca agagattctg ccgccgccgc
     4141 gcccggggaa cacggtcgaa gaaacgttcc cgccctttga aactgaatca ggcccgggtg
     4201 aggtggaatc gggtccgctt caggtgttgc gattctcacc cgaaggcgag atccccatcg
     4261 cgccattcat cagcgtgacg tttaatcaag ccatgattcc gctgggcacc ctgagcgacc
     4321 ttgccgccac ggatgtgcca gtgacgatcg agccgcctct gcccggtaca tggcgctggc
     4381 ttggtaccaa aacgctgacc tttgaatacg actcggatga aattgaccga ctgcccaaag
     4441 ccactgaatt tcacgtcacc atccctgcgg gcacgctttc gatcaatggc aacgcactgg
     4501 ccgaagccgt gacgtggaca ttcaagacgc cgccgcccac cgtggtatcg ttttacccgg
     4561 aggatgcgcc tcagccattg gatcctgttt tcttccttca attcgatcaa cggatcgacc
     4621 ctgcggctgt tttgaaaacc atcgcagtga cagcgggcga tcagcaaatt gagatcgtgc
     4681 ctgctacgca ggagcagatc gaagccgatg agcgggtgag tgctctgacc aaagatgcgc
     4741 ccgaaggccg ctggctggct ttccgtgcca cgcaaccttt taagaccgaa acgaggatct
     4801 ctgttgttgt gggtccgcag gttccttcgc tcgaaggccc gctgaccacg accaccacac
     4861 agacatttgg cttttccacc tactcgccat tgcgtgtgac cggacaacga tgttcatggt
     4921 atgaagatcg ctgcccgccg ctctctccct tctttatcac gttcaacaat ccgctcgatc
     4981 tggattcttt tagtgaagat atgctgagcg tatcacctga gatccccggc gtttctgcca
     5041 gtgtgtatgc cacagatctt cagatcagcg gtgaaacacg cggacaaacc acctacacgg
     5101 taactgtttc tggaaaaatc agagatatct tcgggcaggc attgggcgaa gaccagcagt
     5161 tgaaattcaa agtggataag gctgacccga ttctggtgaa cccggagcag accctggtta
     5221 cgctcgaccc atcatccaaa gagccgctgt attcggccta tgtgatgaat tacaacaaac
     5281 ttaatttgaa ggtctatgcc gtacagcctt cagattggcc gcaataccgc acctatctgc
     5341 gtgaatggca gcaggacaac tcaaatgcaa aaatgccagg caggctggtg gaagagaaga
     5401 ccctcaacct cgatttcccc gatgacaccc tctctgaggt ggagatcaaa ttgcgcccat
     5461 acatggatgg ggagttcggg cacttcgtcc ttgtgatcga accgcctgcg ggaatcttcg
     5521 aaagcgatga cgagcaatgg cggcggtttt cgcagaccat tgtcatctgg gtgcaagtga
     5581 cgcagatcgg ctttgatgtg tacaccgatc acagcgaaat gatcgcctgg acgacgaatc
     5641 tcaaagatgg tgcgccgttg actggtgtga agattatgcc tgagaatggc ggcccgcaga
     5701 ctttgagcga tgccgaaggc attgcccgtt ttgacattcc caacggcgct acatatctca
     5761 ccgcttcact tggcgccgat acggttctcc tgccgcgcag cggttattac tgggacgagg
     5821 ggggctggtc ggctgaggct ccgcttgata cggtgcagtg gttcgttttt gatgaccgtc
     5881 agatgtaccg ccccggtgaa gaggttcacc tcaaaggctg gctgcggcgc gtcggcggca
     5941 ggcagggcgg cgatgtggaa ttgccaggcg atgcagtgcg ttcggtcagt tatcagctct
     6001 ttgaaccgca ggggaatttg atcggtgaag gcaaattgga tgtgaattcg ctcggcggat
     6061 tcgaccttgc tttcaccctg cccgaaggaa tcaatttggg gtttgcccaa ctctacctga
     6121 ccgccgaagg agatgtaaac ggcctgacca acttgcagta ttttcattca cttgcagttc
     6181 aggaattcag gcggcccgaa ttcgaagtca ctgcgcggaa tgaaacagca ggaccgtact
     6241 ttgcgggaga acaggccacc gttgccgtgg atgcgaaata ttacgccggc ggcggcctgc
     6301 ccggtgctga tgtccgctgg aatgtggcca gcaaccctgg cacatactcg ccgcccaatt
     6361 ggcccgattt cacctttggc acccaaagga actggtggtt attttctgat ttcctgtatc
     6421 agaatggctc gttttcacaa ggtgaatatt ttgaagggca aaccgatgcc ttgggcacgc
     6481 actatctgaa cctgaatttc aagccgcagg gcgaccccga taaaaatcca cagccataca
     6541 ccatcacggc cagtgccact gtcattgatg tcaaccgtca ggcatggagc agcggcacat
     6601 ccctgcttgt tcatcctgcc gatgtgtaca tcgggctgcg taccgcaagt tattttgtcg
     6661 aacgcggcga agcgctcaac gtggagtaca ttgtcaccga ccttgatggc aacccggtga
     6721 aaggccgcga agttgtgatc acggccgggg tgttggattg gaaattcaaa aatggagatt
     6781 gggttgaaga agtggtggat gttcaaacct gctcgcttct ttccacgccc gagcctggac
     6841 tctgttcctt tgaaactccc cttgggggaa gttatcagat caccgccctc gttaccgatg
     6901 agcttggacg caagaatcaa agccgtattt tgcgttgggt gagcggcggt aaacaaccgc
     6961 cttcgcgcaa cctggaacag gaagaggtca cactcattcc tgacaaggaa aattatcaac
     7021 ccggcgacat tgcgaagatc cttgttcagg ctcccttcag ccccgccgaa ggcctgctga
     7081 ccgtcacccg caacggcatt ttatacacca cacgcttcac cgtggaagat ggcaccgcca
     7141 cactgcgcat ccccattgaa gaggaacaca tccccaactt gaacattcaa gtggatctgg
     7201 tcggctctgc gccgcgcacc ggagatgacg gtgaaaccct cgaaaacatt ccgccgcgcc
     7261 ccgcttttgc cagcggaaat atgaccctga acattccgcc cgaacagcgg accctgtctt
     7321 tgcaattgac tcccgcccaa tccacgcttg aaccgggcgg tgagaccaac ctgaccgtgt
     7381 tggtgaagga tgcaaatggc aaacccgtag ccgatgcaga actggccgtg gtggtggtgg
     7441 atgaagccat ccttgcgctg acaaattacc aaatgaaaga cccgctcagc attttctaca
     7501 caacacggtc atcctatctt tcaagttatt acagccgtgc cagcctcatc cttgccaatc
     7561 cacaatcatt ggctgcacaa tcactgtcaa ggcaggcggc tgattccgtg ttgtacgctg
     7621 ccgcaaccga agcacctgca tcggttgaaa tgccgatgtc tgaagcgtta acgctgaccg
     7681 ggggcggcgg cgaaaccctg ccagatgcgg ccattgccgt gcgtgcaaat ttcaatccgc
     7741 tggcagcttt tgagccgacg atccgcaccg ggtcgaatgg agaggcgcgg gtgttcattc
     7801 aactgcccga taacctcaca cgatatcggg tgatggtggt tgcggtggat gatggcggaa
     7861 gccgtttcgg cgtgggcgaa tccaacctta cggcacgttt gccgttgatg gttcgtcctt
     7921 ctgcgccgcg cttccttaat tttggcgata cgttcgaatt gccggttgtg ctccagaacc
     7981 agaccaatga accgatgatg gtggatgtgg ttgcccgcgc aaccaacctt gcacttgaaa
     8041 atgcgggcca gcgtgtcacc gtcccagcca atgacagggt ggaggtgcgt ttcaatgctt
     8101 cgacgctcat ggcgggcgag gttcaaattc agtttgcggc ggtttcaggg gggtatgccg
     8161 atgccgccac ggccaacctg cccgtgtaca cccccgccac cagtgaagcc ttcgccacct
     8221 acggcgtgct tgatgaaggt acgatcgccc agcctgtgca atacccaagc ggtgtatttc
     8281 cgcaattcgg cgggttggag gtcagcacat cgtccacggc gttacaggct ttgaccgatg
     8341 ccgtgttgta tctggtgcag tatccgtacg aatgttccga gcaggtggca tcgcgtattc
     8401 tggcggtggc ttcgctgcgc gatgtgcttt ccgcgttcaa ggctgagggg ctgcccacac
     8461 cgtcagaaat ggaagcagct gtcagcgtgg atattgaccg cttgcagtcc atgcaaaatg
     8521 ccgatggcgg gtatccgtat tggcggcgcg gatttgagtc gagtccgttc aacaccatcc
     8581 atgtgaccca tgccctgttc cgcgcacagg aaaaaggatt caatgtgcct gtggagatgc
     8641 agcagaattc attgagttat ctgcgcgata tcgaaagcca ttacccttca tggtatggca
     8701 atgaaacgcg ctggacgctc agtgcgtatg cgttgtttgt gcgcaacctg atgggtgacc
     8761 gcgattcgca gaaggccgaa gcccttttga acgaagctgg cctggagaat cttccgatgg
     8821 aggccatcgg ctggttgtgg tctgtgattg atgatgcaga acaattggat gcgatccgac
     8881 tccatgtgaa caaccgtgtg gttgaaacgg caggcgcggc caatttcacc actgcctaca
     8941 ccgagcagac ctatctcctg ctcagttccg accgccgtac cgatgcgatc ctgctggatg
     9001 ccttgatcga ggatgatccg caaagcgatt tgatcccgaa actggtcaat ggcttgctcg
     9061 cggcggcgcg tcagactcag ggacgctggg gcagcacaca agaaaatgtc tttgtgctga
     9121 cggcgctcga ccgctacttt aacacctacg aatcgcagac gcctgatttt gtggcgcgca
     9181 tctggctggg cgatacctac gcgggcagca atgagttccg cgggtacacc accgatgtct
     9241 ctgagacctt gatcccgatg aattatgtgc tgtcagaaac gtcatcgggc ggcgggatgc
     9301 aggatctgat cctgagcaag gatggccccg gacggttgta ttatcgcctc ggcttgcaat
     9361 atgcgcccac tgaattagac ctcgatccgt tggatatggg ttttgtcgtt gaacgccgtt
     9421 atgaaggggt ggatgacccc gaagatgtga cgttggatgc cgatggaatc tggcatgtca
     9481 aagcgggtgc gcgtgtgaag gtgaccgtca ccatggctgc ggataatcgc cgctatcacg
     9541 ttgcattggt tgacccattg cctgcagggt ttgagatcat caatccgtca ttggctgtct
     9601 ctcaacctgt gccgcaagac ccaaccagcc ccgatttcaa atatggttgg tggtggtggg
     9661 gaccgtggta cgaacatcaa aacatgcgcg atagccgcgc cgaagccttc acctctttac
     9721 tgtgggatgg cgtttatgaa tatgtgtatt atgtccgcgc caccacgccg gggacattca
     9781 ttaccccgcc tgccaaggcc gaagaaatgt actcgcccga agtctttggc cgcagcggca
     9841 cggatcgggt gatcgtgaag taaa
//
LOCUS       DAOQLC010000142         9858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2687_length_9858_cov_20.609201, whole genome shotgun sequence.
ACCESSION   DAOQLC010000142 DAOQLC010000000
VERSION     DAOQLC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2687_length_9858_cov_20.609201"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1054)
                     /locus_tag="PL000_18185"
     CDS             complement(86..1054)
                     /locus_tag="PL000_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HNC90866.1"
                     /translation="MALLASLFFGFVPMFLFAAFVYWLDRYEKEPKLLLGAAFFWGVV
                     IAAGGSYILNTAFGIGIFMFTGSEGAADFGTTSIVAPIIEEFLKGLAVAIVFFMFRKE
                     FDTILDGIIYGGIVALGFAATENTLYIYRNGYQENGWGGLIVLVFIRVILVGWQHPFY
                     TSFTGIGFAFSRTQRNPLIKFIAPFLGYGVAVTTHAFHNTFGGLIGGFEGLFAGTFVD
                     WIGWGLMLGFIIWMINNERNIVKRQLYDEVTNGIISAAHYQRALSPWTLTMAGLSGRA
                     TSRFYQACGELAHKKEQLARHGDESGNVAIINSLRTELTALAPQVR"
     gene            complement(1066..1686)
                     /locus_tag="PL000_18190"
     CDS             complement(1066..1686)
                     /locus_tag="PL000_18190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNC90867.1"
                     /translation="MRVRITFTKQGALRYTGHLDLHRLWERAMRRADLPITYSQGFHP
                     QPRMSLAAALPLGFSSRCEMLDVRLNDEIPLEDIRVRLKDNLPHDIQVTNIESVDERA
                     PALQTQVVSAQYHVQLTEPVDGSDLKRRVAELLESESIPRERRGKFYDLRPLIEALTA
                     DIESSTLQMTLAAREGATGRPDEVLTALGIEPEYARVERTRLIFQE"
     gene            complement(1689..2213)
                     /locus_tag="PL000_18195"
     CDS             complement(1689..2213)
                     /locus_tag="PL000_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90868.1"
                     /translation="MNKSLFLPRLQRERDKFELLLNQVGFSRRMTMKGVVDGLSVKDM
                     LADILSREQFIADRLNEILQGEVYSPCASHSALDAFQSKFGYPDYESPLLNQEARNHL
                     VIHRHKNIGLDDIVEQELAAYENILSAFEHLTHNQCLDHDLFHRTAEHTYKVYRRASL
                     EIQTWLKSIATESN"
     gene            complement(2215..4161)
                     /locus_tag="PL000_18200"
     CDS             complement(2215..4161)
                     /locus_tag="PL000_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNC90869.1"
                     /translation="MMNPEQIELKLDRILLKVQKPGRYVGGELNSIVKDWEKAKTKVA
                     FVFPDIYDIGISNVGIKILYDQINQREDALAERAYAPWTDMEELMREHGIPLYTLESK
                     QPLACFDLIGFTLPYETLYTNTLNILDLAGIPVRSADRNESHPIIIAGGHSTTNPEPM
                     HAFIDAFVIGEGEEVIHDVVDTIQKFKSSPSYKRSELLVALAQISGVYVPKFYETTYL
                     EDGTVAHTLPTIPEAPKLVNKRIVAKLLPPPVKFIVPNIEVIHNRVSVEIMRGCTRGC
                     RFCQAGMITRPVRERSVQEVVDAADAAVRSTGFEELALMSLSSSDYTHISELVDAISK
                     RFEGRKLSVNLPSLRIESVSVDLMDKLKQHRSAGFTLAPEAASERMRRIINKFIPDED
                     IINTTREIYSRGWTTIKLYFMIGHPSETVEDVQAIVDLCRRVIEEGRKVAGWKVKLHA
                     GVSTFVPKPQTPFQWVSCDTKENILEKQALLKRQLLKDKSIRLSWTKPEDSLLEAWLS
                     RGDRRMAEVIYTAWQNGAKFDAWDEAKKQEAWLPAFEAHGLDPAFYTHRQRRTDEVFP
                     WEHITAAVRKNFLFQDFRQSLEGQIRVDCRLNCFACGILPTFANLRRENPGEGWKCPE
                     VKSPVRVTSNQLSVISEQSPLAGD"
     gene            complement(4248..5567)
                     /locus_tag="PL000_18205"
     CDS             complement(4248..5567)
                     /locus_tag="PL000_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90870.1"
                     /translation="MKTIHKLFSIFSLIAILALAFATPAQAFDGRTGNKVVIEKDEVI
                     EDDVYVNAETFILEGTVKGDLVVFGTNITINGVVEGDLIAAGQSVAINGSVSDDARIA
                     GAALLISDSAVIGGDVVGGSASFETKEGSSIEGELVIGAAQALLAGNVTGDALLGLGA
                     LDLRGEFGGNVLANVGEPTEEAGPPFSMFMPQVPFSMPTVNPGITIAEGAKINGNFDY
                     TQFADIDIPTDVVGGKVTRTEPVIDPEETITPPTQSELIVDWSLNLLRTIVTLLILGL
                     LLGWLAPTFIKTLVEKVQTQAAISLGWGVVAYAVFFFAIFIVVVAMVIGGILFGALQL
                     GSLSGTIIVVGLLIIFALIVGFVLATAFLTKIVISWLGGKLILARINPALAESKVWPL
                     ALGVIIVALLTALPGIGWLANLLVVLLGLGALWLWGRETMQARKTAA"
     gene            complement(5636..6328)
                     /locus_tag="PL000_18210"
     CDS             complement(5636..6328)
                     /locus_tag="PL000_18210"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNC90871.1"
                     /translation="MNKEFFDVIKRGYLEVVQRLLSSNPDLIYEKENGLSPVMVALYH
                     REPKIVNYLAEKTGSLNIFEASALGRTTQVVRHLARDPMLVNAYTDDGFQPLGLACFF
                     GHAETAEYLIKAGASINSPSRNQLRATPIQSAAAAGHVEIVLLLLKNRADPNVREQGG
                     YTPLHAAAQNGDVAMIRSLLFNGADLTIRSREGKLPIDMAVESGHVDAAKLLKEGITR
                     RFRTKITLQRTE"
     gene            complement(6442..7557)
                     /locus_tag="PL000_18215"
     CDS             complement(6442..7557)
                     /locus_tag="PL000_18215"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC90872.1"
                     /translation="MKTFKAFLAFTLLFLTGCGTTEMEVPMPSESNSTTPNQQVDEFF
                     AEFVSDNTPGGAVIVVQNGKILHHAAYGLANLEEGTPLTTDHIFHLASVGKQMTALGI
                     LMLVEDGSLNLDDPVGIHVPEVAHFGNEFTIRHLLHHTSGLPDYDDQITDALYTRSDT
                     PTNEDLVAALSEMTELPSTPGDSFEYSNPGYDLLAVVIERVSGENFPDFMKSHIFDPA
                     GMTHTFSLPNEKRRADPLVAMSYTNESGTPEAYPYDDLDNLYGSGSIYSTTTDMALYD
                     EALYTEKFVSQALLAEAFLPTELNDGSIEPYGFGWELEEWNGENYVAHSGAWLGFNSD
                     YIRFPERHLAVIVLLNRDYDYPDDPRIALKIAEYFLQ"
     gene            complement(7554..8090)
                     /locus_tag="PL000_18220"
     CDS             complement(7554..8090)
                     /locus_tag="PL000_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC90873.1"
                     /translation="MTEESLDIVNDNDIVTGQAPRSTIHQNGLQHRGAHVFLFTPEGK
                     MLIQKRSADRTSAPSLLDCSVSEHVKAGESYLEAARRGLREEMGVTVEELTPLVKFKM
                     IYGPNDNEISTLYKGLVDPASVKFDPVEIAEIRYLSMDELRSVLENEKQTLCGWFVEI
                     LHWYWGHPAAMTILKAWS"
     gene            complement(8214..8972)
                     /locus_tag="PL000_18225"
     CDS             complement(8214..8972)
                     /locus_tag="PL000_18225"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC90874.1"
                     /translation="MSKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWSVEY
                     YHELLQIAGGKERMKHHWKTKGFSKPLTEEEIDNLVKEMHKRKTALFVELIESGKLPL
                     RPGIHRFMKELMEAGIKIGVCTTSNEQAAKAITEGILSDIKFEVVLAGDVVEKKKPDP
                     EIYNLGLAKLELKPEEAFVVEDSKNGVKAAKAAGLKTIVTTNPYTEKEDVEAGDVIVS
                     CLGDPDGEKAKMRKGDIKDFDGIVHAKQLIELFG"
     gene            complement(9057..>9858)
                     /locus_tag="PL000_18230"
     CDS             complement(9057..>9858)
                     /locus_tag="PL000_18230"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC90875.1"
                     /translation="IEPGDEVIQHTPCFGIYGLRGTLLGAKMVSVPMIYEDHKLKFDP
                     DGLMKAVTDKTKIIVIPNPNNPSGNFMDPEHFEPFAKLGIPLVVDEAYIEYAGLGKSQ
                     VELTKKYKNVFITRTLSKAYGLAGMRFGYTIAHEDTLKQVAGSLLPWNVGTIPMWAAL
                     AAFEDTEGLAERVKFNNDAVDYISESLSVIPGFYVMQSKANYILFDCGETGKTGKQVL
                     EYAETKGIILRGESKKYGSDGWFRVTIGSKEENELFVNTVLEFFGMKK"
BASE COUNT         2215 a         2353 c         2802 g         2488 t
ORIGIN      
        1 gggattatat cgcacgggag ggaaagcaaa aggctgacga aacatcgcca gcctttgaag
       61 attttagtcc gtgctttgga gttttttacc tgacttgcgg agccagcgcc gtcaattctg
      121 tgcgcagcga gttgatgatg gccacgttgc cgctttcatc gccgtgcctg gcgagttgtt
      181 cttttttatg ggccagttct ccgcatgctt gatagaagcg tgaggtggct ctgccgctca
      241 agcctgccat ggtcagggtc cacggggaga gcgctctttg gtaatgcgct gctgaaatta
      301 ttccgttcgt cacttcatcg taaagctgcc gcttgacgat attgcgctcg ttgttgatca
      361 tccagatgat aaagccgagc atgaggcccc agccgatcca atctacgaag gtgcctgcga
      421 aaaggccttc gaaaccgccg atcagcccgc cgaaggtgtt gtgaaaggca tgggtggtga
      481 ctgccacacc gtagccgaga aagggtgcga tgaatttgat caacgggttg cgttgggtgc
      541 gtgaaaaggc aaatccgatc cctgtgaaag aggtgtagaa cggatgctgc catcccacca
      601 gaatgacgcg gatgaagacg agcacgatca ggccgcccca gccgttttcc tgatatccgt
      661 tgcggtaaat gtagagtgtg ttttctgtgg cggcgaaccc gagcgccaca atgccgccat
      721 aaataatgcc atcgaggatg gtgtcaaatt ccttgcggaa cataaaaaat acaattgcca
      781 ctgcgaggcc cttgagaaat tcctcaatga tgggggcaac gatggatgtg gttccaaagt
      841 ccgcggctcc ttcagagccc gtgaacatga aaatgccgat gccaaaagcc gtgttgagga
      901 tgtatgaccc gccagccgcg atcaccacac cccaaaagaa agccgcaccg agcaaaagtt
      961 tcggctcttt ttcataacgg tcaagccaat acacaaaggc cgcgaataaa aacatcggca
     1021 caaaaccaaa gaatagtgaa gcgagcagag ccatctgatt tctccttatt cttgaaaaat
     1081 taagcgggtt ctttccacac gcgcatattc aggctcaatg ccaagcgcgg tcaatacttc
     1141 atcggggcgg cccgtggcgc cttcacgtgc ggcgagggtc atttgcaggg tggaggattc
     1201 tatatctgcg gtcagggctt cgatgagcgg acgcaggtcg tagaattttc cacgacgttc
     1261 gcgcggaatc gattcggatt cgagcaactc tgcgaccctg cgcttcaaat cggacccgtc
     1321 aactggctct gtcaattgga catggtattg cgccgagacc acctgtgttt gcagggcagg
     1381 cgcacgctcg tccacgcttt cgatgtttgt cacctgaata tcatgtggaa gattatcctt
     1441 caaacgcacc ctgatatctt ccagcggaat ctcatcgttc aggcgtacat ccagcatttc
     1501 acagcgtgaa gagaagccca gcggcagcgc ggcggcaagg ctcatccgcg gttgggggtg
     1561 aaagccttgt gagtaggtga tgggcaggtc ggcgcggcgc atggcgcgct cccagagacg
     1621 gtgcaggtca aggtgacctg tgtaacgcag ggcgccttgt ttggtaaatg tgatgcgaac
     1681 acgcatgttt aatttgattc cgttgcgatg gattttagcc aggtctgaat ttcgaggctg
     1741 gcgcggcgat agaccttgta ggtgtgttcc gcagtgcggt ggaaaaggtc atggtcgagg
     1801 cattgattgt gggtcaggtg ttcaaaggcc gaaaggatgt tttcgtaggc ggcgagttcc
     1861 tgttccacga tgtcatccaa accgatgttc ttatgcctgt gaataaccag atggttgcgg
     1921 gcttcctgat tcagcagggg tgattcgtaa tcagggtaac cgaatttgct ctggaatgca
     1981 tcgagtgcag agtgtgaagc gcagggagag taaacctctc cctgcaggat ttcatttaag
     2041 cgatcggcaa taaactgctc acggctgaga atatccgcca gcatatcctt gaccgataat
     2101 ccatccacga cgcccttcat tgtcatgcgg cgcgaaaaac caacctgatt caggagcaat
     2161 tcgaacttat cgcgttcgcg ttgcaagcgt ggaagaaaaa gactcttgtt caatctagtc
     2221 acccgccaat ggcgattgct cactgatgac cgacaattga ttgctggtca cccgtacggg
     2281 gctcttaact tcaggacact tccagccttc ccctgggttt tcgcgtcgca ggttggcaaa
     2341 ggtcggcaaa atgccacagg caaaacaatt caagcggcaa tccacgcgga tctgcccttc
     2401 gagcgattgg cggaaatctt gaaagaggaa attcttgcgc acggcggcgg taatgtgttc
     2461 ccacgggaag acctcgtcgg tgcggcgctg acggtgggta taaaacgcgg ggtcgagccc
     2521 gtgtgcttcg aaagcaggca gccaggcttc ctgtttcttg gcttcatccc aggcatcgaa
     2581 tttcgcgccg ttctgccagg cggtgtagat cacctctgcc atgcggcggt caccgcgcga
     2641 aagccaggct tcgagcagag agtcttcggg ttttgtccaa ctcagcctga tgcttttatc
     2701 tttcaaaagc tggcgtttca gcagggcttg tttttcgagg atgttttctt tggtatcgca
     2761 agaaacccac tggaaaggcg tttgcggttt gggcacaaag gtgctcacac ctgcatgcag
     2821 tttcaccttc caacctgcca ctttacggcc ttcttcaatg acgcggcggc acagatctac
     2881 aatggcttgc acatcttcca cggtttcgct ggggtggccg atcatgaaat acagtttgat
     2941 cgtcgtccaa cctcggctgt agatttcgcg ggtagtgttg atgatgtctt catcgggaat
     3001 gaacttgttg atgatgcggc gcatccgttc gctggcagct tcaggcgcaa gggtgaaacc
     3061 tgctgaacga tgctgtttca gcttgtccat cagatccaca gagaccgatt cgatccttag
     3121 cgacggcaaa ttcaccgaga gtttgcgccc ttcgaaacgc ttgctgatgg catccaccaa
     3181 ttcgctgatg tgagtgtaat cggatgacga gagcgacatc aatgcgagtt cttcaaaccc
     3241 cgtagagcgg actgccgcat cggcagcatc caccacctct tgaacgcttc tttctctaac
     3301 aggacgcgtg atcatacccg cctgacagaa tcgacaccct cgggtgcatc cccgcatgat
     3361 ttccaccgaa acacggttgt gaatcacttc aatgttcggc acaatgaatt taacgggcgg
     3421 cgggagcaac tttgccacaa tacgcttgtt taccagcttt ggcgcttcgg gtatggtggg
     3481 cagggtgtgc gccacagttc catcttcaag ataggtggtc tcgtagaatt ttggcacata
     3541 cacacctgag atctgggcaa gcgccaccag caattcactc cgcttgtagc ttggagacga
     3601 tttgaacttt tgaattgtgt caacaacatc gtgtatgact tcttcacctt caccgatgac
     3661 aaatgcatca ataaaggcat gcatcggctc ggggttggtg gtggaatgtc cgcctgcgat
     3721 gatgatgggg tggctttcgt tgcggtctgc tgaacggacg ggaatgcctg caaggtcgag
     3781 aatgttcagg gtgtttgtgt agagggtttc gtaagggagc gtaaagccga tgaggtcgaa
     3841 acaggccagg ggttgtttgg attcgagcgt gtagagcggg atcccgtgtt cacgcatcaa
     3901 ttcttccatg tcggtccacg gtgcataggc acgttctgcc aatgcgtctt cacgttgatt
     3961 gatctgatcg taaaggatct taatgccgac atttgaaatg ccgatgtcgt aaatatcagg
     4021 gaagacgaag gcaacctttg ttttggcttt ttcccaatct ttgacgatgc tattaagctc
     4081 gccgcccacg tatcgaccgg gcttttgtac ctttaaaagg attcggtcaa gttttaattc
     4141 gatctgttct gggttcatca tgggcgggat tataacaaag aacctccgat taaaaagaaa
     4201 gcggatggac ttgtgagggt ccacccgctt ttgaggggaa gatgaaatta agcggcggtt
     4261 ttgcgggctt gcatggtttc gcggccccaa agccagaggg cgccaaggcc aagtaaaaca
     4321 acgagcaggt tagccagcca gccaatgcct ggcaatgcgg tcagcagagc gacgatgatc
     4381 acgccgagcg caagcggcca tactttactt tcggccagtg cagggttgat gcgcgcaagg
     4441 atcagtttgc cgcccagcca ggaaatgacg atcttggtaa gaaaggcggt tgccagaaca
     4501 aagccaacga tcaatgcaaa gatgatgagc agaccaacca caatgatcgt gccgctcagg
     4561 ctgccaagtt ggagtgcgcc gaagaggatt ccgccaataa ccatggccac gacaacgatg
     4621 aagatggcga agaagaagac cgcgtaggca accacgcccc agcccagtga tatggctgcc
     4681 tgggtttgga ctttttctac cagggtcttg atgaaagttg gagcgagcca gcctagcagc
     4741 aatccaagga tcaggagggt cacgatggtg cgtaacaggt tgaggctcca atccacgatg
     4801 agttcagatt gtgtgggtgg ggtaatggtt tcttctgggt cgataacggg ttcggtgcgt
     4861 gtaacctttc cgccgacaac atcggtcgga atatctatgt cggcgaattg ggtgtaatca
     4921 aagttaccgt tgatctttgc accttcagca atggtaatgc cagggttgac ggtaggcatg
     4981 gaaaacggta cctgtggcat gaacatggaa aatggaggac cagcttcttc agtgggctca
     5041 ccgacgttgg cgagtacgtt gccgccaaat tctccgcgca agtcaagcgc tcccaagccg
     5101 agcagtgcat cgccagtgac attgcctgcc agcagggctt gtgcggcacc gatcaccaac
     5161 tcaccttcga tggaactgcc ttctttggtc tcaaagctgg cactaccgcc aaccacatct
     5221 ccgccgatta cggctgagtc gctgataaga agtgcggcac ctgcgatacg ggcgtcatct
     5281 gaaaccgagc cgttgatcgc cacgctttgc ccggcggcga tcaaatcgcc ttcaacaacg
     5341 ccattaatgg tgatattggt tccaaatacc accaaatcac ctttgactgt cccttccagt
     5401 atgaaagttt cagcgttgac atatacatcg tcttcgatca cttcatcttt ctcgatgacg
     5461 actttattgc ctgttcggcc atcaaatgcc tgggcagggg tggcgaaagc aagggcaagg
     5521 atggctatga gggaaaaaat ggaaaaaagt ttatgaatgg ttttcatggg ggctcctttg
     5581 aaaaaaactt cccgatgtag gtatacgtgg ccatcatcgg gaggttgcat tcattttatt
     5641 cggttctttg gagggttatt ttcgtcctaa aacggcgggt gatgccttct ttgaggagtt
     5701 tcgccgcatc cacatgacct gattcgactg ccatgtcgat ggggagtttt ccctccctgc
     5761 tgcgaatcgt caaatctgca ccgttgaaga ggagcgagcg gatcatggcg acatcgccat
     5821 tttgtgcggc ggcatgcagc ggcgtgtagc caccttgttc gcgtacattt gggtctgcgc
     5881 ggtttttgag gaggaggagc acgatctcaa catggcctgc ggcggcggct gattgaattg
     5941 gggtcgccct taattgattt ctagaggggg agtttatgga ggctccagcc ttgatcaggt
     6001 attcggcggt ttcggcatgg ccaaaaaaac atgccagccc aagcggttga aaaccatcat
     6061 ccgtataggc gttgacgagc attgggtcgc gggcaaggtg gcgcaccacc tgtgtggttc
     6121 tgccgagggc cgaagcttcg aagatattca ggctgcccgt tttttcggcg aggtaattca
     6181 cgatcttcgg ttcgcgatga tagagggcca ccatgaccgg gctgagacca ttttcttttt
     6241 catagatcag gtcggggttt gatgaaagga ggcgctggac cacttcaagg tacccgcgct
     6301 tgatcacgtc aaaaaattcc ttgttcattt gatgatcact ccatttcctc tgtaggattt
     6361 acccgcatgt ggataattaa tgaccgatta tacgtctgat ggtttgtgaa gctgtttgca
     6421 atcctgttag ccgtttttga gttattgaag gaagtattca gcgattttta gagcgatgcg
     6481 tggatcgtct ggatagtcat aatcacggtt aagtaaaaca atgaccgcta aatggcgttc
     6541 gggaaaacgg atgtaatcgg agttgaaccc gagccaggca cccgaatgag caacgtaatt
     6601 ttcaccattc cactcttcca gttcccagcc gaagccgtaa ggttcgatgc tcccatcgtt
     6661 taattctgtg ggtagaaatg cttcggccag cagcgcttgc gagacgaatt tttcggtgta
     6721 aagggcttca tcgtacaaag ccatatctgt tgttgtggaa taaatggagc ctgagccata
     6781 gagattatcc aaatcgtcgt aggggtaggc ctcgggtgtt ccactttcat tggtatagct
     6841 cattgcaacc agtgggtcgg cgcggcgttt ttcgtttggc agggagaagg tgtgggtcat
     6901 gccggcaggg tcaaagatat gggatttcat gaagtcaggg aagttttccc cggaaacgcg
     6961 ttcgatgacc actgccagca ggtcgtaacc gggattgctg tattcaaatg aatctcccgg
     7021 ggtggagggt aattcggtca tttctgagag agcggcgaca aggtcttcat ttgttggggt
     7081 atctgaacgg gtgtaaagcg catcggtaat ttggtcatcg taatcgggca ggcccgaagt
     7141 atggtggaga agatggcgga tggtgaactc attgccaaaa tgagctactt cgggaacgtg
     7201 aatgcctacc gggtcatcca ggttcaagct gccatcttca acaagcatga gaatgccgag
     7261 ggctgtcatt tgtttgccaa cggatgcgag gtgaaagatg tggtctgtgg tgagcggtgt
     7321 gccttcctcc agattggcca atccataggc ggcgtgatga aggattttcc cgttttgcac
     7381 aacgattacg gcaccgcctg gggtgttgtc actgacaaac tctgcgaaga attcatccac
     7441 ttgttggttg ggtgtggtgg aatttgattc agatggcatg ggaacttcca tttctgtggt
     7501 gccgcagcca gtcaggaaga gcagggtgaa agcgaggaag gctttgaagg ttttcatgac
     7561 cacgccttga gaatagtcat cgcggcgggg tgcccccaat accagtgcag aatttcgaca
     7621 aaccagccgc atagggtttg tttttcattt tccaacacgg atcgtaattc gtccatgctg
     7681 aggtagcgga tttcggcgat ttcgacaggg tcaaatttca cactggcggg gtcaacgagg
     7741 cctttgtaga gggtgctgat ctcgttgtcg ttggggccgt agatcatttt gaacttcacg
     7801 aggggggtga gttcttcgac cgtgacgccc atttcttccc gcagcccgcg tcgggcggct
     7861 tcgagatagc tttcacctgc tttgacatgc tcggagacgg agcaatccag cagagatggg
     7921 gcggaggtac ggtcggcgct gcgtttttgg atgagcattt tgccttcggg tgtgaaaagg
     7981 aaaacatgcg caccgcgatg ttgtaatcca ttttggtgga tggttgagcg aggggcttgc
     8041 cctgtgacaa tgtcattgtc gtttacgatg tcgagggatt cttctgtcat gtttttttga
     8101 ggggagaaaa tatcaatggt ggtggagcag gttgagggtg agttggaggc ggggtttgat
     8161 ttggaaaaag aaagaggcgc ggaagagcga tccgcgcctg cttgttttgt gggctatcca
     8221 aacagttcaa tgagttgttt ggcgtgtacg atcccgtcaa agtctttgat gtcgcctttg
     8281 cgcattttcg ccttttcgcc gtcggggtcg ccgaggcagg agacgatgac atcgcctgct
     8341 tccacatctt ctttttcagt gtagggattg gtggtgacga tggttttcag gccagcggct
     8401 ttggcggctt tgacaccgtt cttggagtct tcgaccacaa aggcttcttc gggtttcaac
     8461 tcaagtttgg caaggccgag gttgtagatt tctggatcgg gtttcttctt ttcaaccaca
     8521 tcgcctgcga gcacaacttc aaatttgata tcggacagga tgccttctgt gatggctttg
     8581 gcggcctgtt cattggaggt ggtgcagacc ccgatcttga tgccagcttc catgagttct
     8641 ttcatgaagc ggtgaatgcc ggggcgcagg ggaagtttgc ccgattcgat caactcgacg
     8701 aaaagggcgg tcttgcgttt gtgcatttcc ttgacgagat tatcaatctc ttcttcggtg
     8761 agcggcttgg aaaacccttt ggtcttccag tgatgtttca tgcgttcctt gccgccagcg
     8821 atctggagta attcgtggta gtactccacg ctccactcgt cggtgaagcc gaattccttg
     8881 aaggtcatat taaacgatac gcggtgacca tcgcgttcag tgtcgatgat cacgccatcc
     8941 tggtcgaaga aaactgcttt tattttggac atggtttcct ctttctcgcc acaaaggagc
     9001 gcgaagggtc acaaagttgt ttttctctgt gatccttcgt gttcttcgtg gaaaccttat
     9061 ttcttcattc caaagaattc aagcacggtg ttgacgaaga gttcgttctc ttctttcgag
     9121 ccgatggtca cacggaacca gccgtcggaa ccatatttct tgctttcgcc gcggaggatg
     9181 atgcccttgg tttcagcgta ttcgagcacc tgtttgccgg tcttgccggt ttcgccgcag
     9241 tcgaagagga tgtagttggc tttggactgc atgacgtaga agccgggaat gacgctcaac
     9301 gattcgctaa tgtaatccac cgcgtcgttg ttgaacttga cgcgctcggc aaggccttca
     9361 gtgtcttcga acgcagcaag ggcggcccac atggggatgg tacccacgtt ccaaggcagg
     9421 agcgaaccgg caacctgttt aagggtgtct tcgtgcgcaa tggtgtaacc gaaacgcatg
     9481 ccagccaaac cataggcctt ggaaagcgtg cgggtgatga acacgttctt gtacttcttg
     9541 gtcaattcca cttgcgactt gcccaaacca gcatattcga tgtaggcttc atccacaacc
     9601 aacggaatgc ctaacttggc gaagggttca aagtgctccg gatccatgaa gttgccgctg
     9661 gggttgttgg gattcgggat gacgatgatc ttggtcttgt cggtgacggc tttcatcaaa
     9721 ccatcggggt cgaatttcag tttgtgatct tcgtagatca tcggcacaga gaccatcttg
     9781 gcgccgagca atgttccgcg caggccgtaa atgccgaaac agggggtgtg ctgaatgact
     9841 tcgtcgcccg gttcgatg
//
LOCUS       DAOQLC010000143         9564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2786_length_9564_cov_24.183931, whole genome shotgun sequence.
ACCESSION   DAOQLC010000143 DAOQLC010000000
VERSION     DAOQLC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9564
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_9564_cov_24.183931"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3434)
                     /locus_tag="PL000_18235"
     CDS             complement(<1..>3434)
                     /locus_tag="PL000_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC90876.1"
                     /translation="EGRAQAMLQAIPDLMFRLNREGVFLDYKADAKDLHEQTVSLIGT
                     RNRDIAPVEFADLIDHYIAKTLEANQLQTFEYQLPVPGKGLRDYEARMTPSGTDEVLA
                     IVRDITERKQVEQNLRETQSRVELALKGANAGMWDWNVQTGETIFNERWAEIIGYALE
                     EIEPVSIQTWADFCHPDDLEISDGLLKKHFAGETQFYECEVRMKHKNGSWVWVFDRGK
                     VMEWDSEQRPLRMIGTHLDITERKQTEEALRAREELYRMISTINADYVFSEDVDMNGK
                     TSTKWVGGGFEAITGYTFEEYSKQGGWLSILHPEDREKDAADFAALKKNQKIVSVLRM
                     IAKNGDIRWVQVHANPLWDSELQCLTGVYGAVQDITERKKAEDALHASEEKYRVLIES
                     LDNMIASVDNDGRFLYVNDMAAAQMHLTPNELIGKTMYELFPPEVTARQMSDIQRVIH
                     GDQGITFEAPSFSSTAPRWYRTSILPIHDEDGLVTQVLINSTDIHNLKTAQQELQDLN
                     RTLEERVKQRTAEVQDLYDNSPIGYHSLDADGTLLFINQTHLTWLGYTREEMIGHSIT
                     EFLTTSGLEGFKEIYPSFLKNGMVRDVESEFRRKDGSIVPVLISATAVYNEAGEFVMS
                     RTSVFDNTGQKAAEQALRNANLELARAMRMKDEFLASMSHELRTPLTGILGLSEALQI
                     NVYGPINEKQQNTLANIEASGRHLLDLINDILDVSKIEAGKLELQMEACSLSEICQSS
                     IQLTKGMAGKKRQTVNFTMNPANISVRGDARRLKQILVNLLSNSVKFTPEEGMLGLDV
                     TANDSEGLVYISIWDKGIGITPEDLKKLFQPFVQLDSSLSRQQSGTGLGLALVQRLVE
                     LHGGSIDVQSVPGEGSRFTVTLPCQPADKASQEVENPSQLKFHQALIIEDTPADAEHL
                     ARYVKSLGITAIMHTSGKDAVERVIETQPDVVFLDLNLPEVSGWDVLTQIKNSRNTKH
                     IPVVITSVDDDKSKASKLDADGYLVKFFTLSDLRTALSHIQKPSLASSEGHINPAAAT
                     VATIMIVDDNEVNINTLADFLAMQNFNIISVKSGVDFLAQAPHIHPDIVLMDIQMPGM
                     DGLETTRRLRRLSDRRLASVPVVAITALAMPGDRERCIEAGADEYITKPFRLTEIH"
     assembly_gap    3435..3534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3535..>5660)
                     /locus_tag="PL000_18240"
     CDS             complement(<3535..>5660)
                     /locus_tag="PL000_18240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC90877.1"
                     /translation="FEKIQESEEKYRLLFETMVQGVVFQDENGGIIHANPAAERILGL
                     TLDQMQGRTSIDPRWKSIHEDGSDFPGDTHPSMVALKTGKPIHNVTMGVYNPAFDSYN
                     WININATPRFNGNSEKPHQVYATFEDITERKLAENSLLASEKKLKSLIASQTHFVIRI
                     NMEGKYVYWNPQFEKEFGWIHEPNGLLHADSTKTICEHHRKRVFDVVQQCVSQPGQVF
                     SVEIDKPARNGGIRTTLWEFMCITDEHNQPSEIQCMGMEITDRKKAEEAMRESEAKYR
                     LLAENISDVIWVLDVESSRFRYVSPSVTSLRGYSVEEVMTQDMAATLTPVSLEYLSQV
                     TPERLGKFQQGLSKSYTDEIEQTCKDGSTVWTEVISRYVIDPETNRLEVYGVSRNITE
                     RKKAEAAIRLIEKRNTALIEHAPDGIALVDADGMFLFASPSAYRMFGYDAAEIVGTQS
                     REKVHPQDVSLLANLRGKLLSNPNVPQTVEYRFMHKNGEYHWLESTYTNMFDEPSISG
                     IVINFRDVTERKLSEEITRQQSEQIHLLYEASQKLNRTLNLQEIYQTICDFMVTTIPT
                     STMFISAFDQETQLITCRAYWIENKWLDVSNFPSIPLEEEGKGTQSLVIRTGKAMLLN
                     DFQSYMKNTSTVYQVDSDTNEISSEDDETPEEEVTRSALIVPLKSDGIVRGIIQVSSY
                     RQDAYTENQLKLLESLALHIASAEQNA"
     assembly_gap    5661..5760
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5761..7571)
                     /locus_tag="PL000_18245"
     CDS             complement(<5761..7571)
                     /locus_tag="PL000_18245"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC90878.1"
                     /translation="MSKQKTKLQLQQELELAQKRIEELERKLPAPDENEERFSRFFQA
                     SPAQMAITDINTGKYLDINKTFLETLEFTREEVLGKTALELNLFFDPDQRGALLGKMK
                     EQGFLRDEDVLVRTKSGGLRHGIFSAEYIQVNSQSFLLTVMNDITERKLAEERLSESE
                     ARLKGFLDSAPDAMIIVNAQGKIILANLQTEKLFGYKQSELLEMKIEQLIPERLRDAH
                     PQKRDYYLQNPQHKTTGIQQGIYALRKDGSEFPVEISLSHHKMKNEVVILSAIRDITE
                     KKQAEVFLLKSTQILHESQLMARLGSWTADLKTGIFESSSEDAQLIGWAPGGHSLNEL
                     MNIIHPDDREFVQVSWSAALQGGPFDIEYRIIAGDDIRWLRVKASIKFDNHGVPASAI
                     GVTQDITERKQSAILQETVYHIANAAQTTESLQDFFKGIHHHISNVMAARNFYIALHD
                     EEENLLHFVYSMDEKDPIRESMPPDKGLTSHVLRTGESLLYVYNQTGQRIEVVGTQPK
                     VWLGVPLIARNKIIGVMAVQNYSDPHAYGIREQRILEFVSSQVATAIHLKKATDLLQK
                     SQTSLEMALATANLGSWELDIKRGKGMVWSKEMFHLFR"
     gene            complement(7789..>9564)
                     /locus_tag="PL000_18250"
     CDS             complement(7789..>9564)
                     /locus_tag="PL000_18250"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC90879.1"
                     /translation="IRKLPAVETLGSVTVICSDKTGTLTENRMTVVVLDVADHALDLT
                     EEIERDGTLRATRGLGAPAQSSLSLAVIGGALCNDSKLIDTGDDRFHTLGDPTEGALV
                     VAAAKMGYWKDSLDPSFPRAAELPFDSERKRMTTVHHLGQYDPTVLAGLEIGDHRYIA
                     FTKGGVDGLLDITSHIWSEGKSQILDSGWRSKIEAANERLAKKGMRVLGIAFRLMNSI
                     PEVIQTDLEQNLTFVGLFGMIDPPRGEVQEAVATCRAAGIRPVMITGDHPLTAIEIAR
                     QLGITENGRALTGVEIENLSSTELRSVVDEVNVFARVAPEHKLRIVQALQENGHIVSM
                     TGDGVNDAPALRKADIGVAMGITGTDVSKEASDLVLLDDNFATIVASVKEGRTIYDNI
                     RKFVRFSVAGNIGKVLVMLIAPFLGKPLPLLPLQLLWLNLLTDGLLGLGMGVENPEPD
                     TMKRKPYSPTEGVFSRGAGTQTIWVGAMIGALALGLGSWYFFTGRPEWQTMIFTSLAF
                     MQVFQAFASRSSKESVFKMGLLSNSLLAGMGLLVVVLQLMVMYIPVLAHFFEVMPLSG
                     CDLAIAASTGVIVLTVMELEKKLKK"
BASE COUNT         2070 a         2324 c         2404 g         2566 t
ORIGIN      
        1 tcgtggatct ccgtcaaacg gaagggcttg gtgatgtatt catcggcgcc cgcttcaatg
       61 cagcgttcgc ggtcgccggg catggccaga gcggtgatcg ccaccacggg aacagaggcc
      121 agccgtcggt ctgacaggcg gcgcaaacgg cgggtggttt cgagcccatc cataccgggc
      181 atttgaatat ccatcaagac aatgtctggg tggatatgcg gagcctgggc caggaaatcc
      241 actccgcttt ttacggagat gatattgaaa ttctgcatgg caagaaaatc ggccaatgtg
      301 ttgatgttga cctcgttatc gtccacgatc atgatggtgg caacggttgc tgcggcaggg
      361 ttgatgtggc cttcactgct ggcgaggctc ggtttttgaa tatgacttag ggcggtgcgc
      421 aggtctgaaa gagtgaagaa cttgacgagg tatccatctg cgtccaactt ggaagccttg
      481 gatttgtcat catccacgga tgtgataacg acgggaatgt gtttggtgtt cctgctgttc
      541 ttgatctggg tgagcacatc ccaacctgaa acttcgggca ggttgagatc aaggaaaaca
      601 acatcaggct gtgtttcaat gactcgctcc acagcgtcct tgccgctggt gtgcatgatg
      661 gcggtaatgc ccagcgactt gacgtaacgt gccaggtgtt cagcgtcggc gggagtatct
      721 tcgatgatca aggcttgatg gaattttagt tgagacggat tttcaacttc ctgcgaagcc
      781 ttgtccgcag gctggcaggg cagggtcact gtaaagcggc tgccctctcc ggggacactt
      841 tgaacgtcaa tgcttccgcc gtgcaattca accagtcgtt gtaccaaagc cagcccaagg
      901 ccggtgccgc tttgctgcct tgaaaggctg ctatccaatt gtacaaatgg ctggaatagt
      961 tttttcaggt cttcgggggt gatgccaatg cctttatccc aaatggagat gtataccaag
     1021 ccttcactat cattggctgt gacatccaac ccaagcatgc cttcttccgg ggtgaatttg
     1081 accgaattgc tgagcaggtt gacgaggatc tgcttcaatc gccgcgcatc accacggaca
     1141 ctgatattgg ccgggttcat tgtaaagtta acagtctgac gctttttgcc tgccatgcct
     1201 ttggtaagct ggatgctgct ctggcagatt tcgctgagcg aacaggcttc catttgcaat
     1261 tcgagcttgc ccgcttcgat cttggataca tccagaatgt cgttgataag gtcgagcagg
     1321 tgacgtccgc tggcttcgat atttgcaagc gtgttttgct gcttttcgtt gatcggaccg
     1381 tacacattga tctgcagagc ttccgaaaga cccagaatac cggtcaatgg ggtgcgcagt
     1441 tcatggctca tgctggcaag gaattcatct ttcatgcgca tggcgcgtgc cagttcgagg
     1501 ttggcattgc gcagggcctg ttcagccgct ttttgaccgg tgttatcgaa tacggaggta
     1561 cggctcatca cgaattcgcc tgcttcattg tagactgccg tagcgctgat caataccggg
     1621 acgatgctcc catctttgcg gcgaaattcc gattcgacat cgcgtaccat gccatttttc
     1681 aaaaaggacg gatagatctc tttaaaccct tccagaccgc ttgtcgtaag gaattccgtg
     1741 atcgaatggc cgatcatttc ttcgcgggtg taccccagcc aggtgaggtg ggtttggttg
     1801 ataaacagga gtgtgccatc tgcatccagt gagtggtagc cgatgggtga gttgtcgtac
     1861 aggtcttgca cttcggcagt gcgttgtttt acgcgctctt caagtgtgcg gttgaggtct
     1921 tgcaactcct gctgggcggt tttcaggttg tgaatgtcgg tggaattgat cagcacttgc
     1981 gtaaccagcc catcttcgtc atggatcggc aaaatggagg tgcggtacca gcggggcgca
     2041 gttgaagaaa aactgggggc ttcaaatgta atgccttgat caccatggat tacgcgttgg
     2101 atgtcgctca tttgtctggc tgttacttct ggcggaaaga gttcatacat ggtcttgcca
     2161 attaattcat ttggcgtcag gtgcatttgc gcggcagcca tatcgttaac atacaggaat
     2221 ctgccatcgt tatccacgct ggcgatcata ttatccagac tctcgatcaa cacacggtat
     2281 ttttcttcac tggcatgcag ggcatcttcc gcttttttgc gctcggtaat atcctgcacg
     2341 gctccatata cccctgttaa gcattgaagt tctgaatccc agagcgggtt ggcatggact
     2401 tgtacccagc gtatatcgcc gttttttgcg atcattctga gaacactgac gattttttgg
     2461 ttctttttta atgcagcaaa atcggcggca tccttttctc ggtcttcggg gtggagaatg
     2521 gagagccaac ctccctgttt tgaatattct tcaaaggtgt acccggtaat ggcttcaaag
     2581 ccgccgccca cccacttggt ggatgttttg ccattcatat ccacatcttc tgaaaataca
     2641 taatctgcat tgatcgtgga gatcatgcgg tacagttctt cgcgggcgcg caaggcttct
     2701 tcagtttgtt ttctttcggt gatatccaga tgagtaccga tcatgcgcag cggacgctgt
     2761 tccgaatccc attccatgac cttgccgcgg tcaaataccc aaacccatga accgtttttg
     2821 tgtttcatac gaacttcaca ttcgtaaaac tgggtttcac ctgcaaagtg ctttttcaac
     2881 aaaccatccg atatttccaa atcatcagga tgacagaaat cagcccaggt ttgaatgctg
     2941 accggttcaa tttcctccag cgcataccca atgatctcag cccagcgttc attgaatatg
     3001 gtttcccctg tttgcacatt ccaatcccac atgcctgcat ttgcgccttt cagagcgagt
     3061 tccacccttg attgggtctc acgcaggttt tgttcaacct gttttcgttc agtaatatcg
     3121 cggacaatgg ccaatacctc atcggttccg ctgggggtca tgcgggcttc gtaatcacgc
     3181 aggccttttc cggggacggg caactggtat tcaaatgtct gtaattggtt ggcttccaga
     3241 gtcttggcta tgtaatgatc gatcaggtcg gcgaattcaa caggcgcgat atctcgattt
     3301 ctggttccga tcagggaaac ggtttgctca tgcaggtctt ttgcatctgc cttgtagtct
     3361 aaaaatacgc cttcacgatt caaacggaac atcagatcgg ggatggcttg cagcatggcc
     3421 tgtgcgcggc cttcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcattt
     3541 tgctctgcgg atgcgatatg cagcgcgaga gattccagga gcttcaactg gttttccgta
     3601 taggcatcct gacgataact cgatacctga atgatcccgc ggacgatccc atcggatttc
     3661 agtggaacga tcaaagccga acgtgtaact tcttcttcag gtgtttcatc atcttctgat
     3721 gagatttcat tggtgtcgct gtctacctga taaaccgtac ttgtattttt catatacgat
     3781 tgaaaatcat tgagaagcat ggctttgccc gtgcgtatga cgaggctctg tgtgcctttg
     3841 ccttcctctt caagtggaat ggacgggaaa ttgctgacat ccagccattt gttttcgatc
     3901 cagtaggcgc ggcaggtgat taactgtgtt tcctgatcga atgctgaaat gaacatggtg
     3961 cttgtgggaa tggtggtcac catgaagtca cagatcgtct gatagatttc ctgcaggttg
     4021 agggtacgat ttagcttttg gctcgcctca tacaacaggt gaatctgctc gctttgctga
     4081 cgggttatct cctctgacag tttgcgctcg gtcacatcgc ggaagttgat gacgatgccg
     4141 ctgatacttg gctcgtcaaa catgttggta taggtgcttt caagccagtg atactcaccg
     4201 ttcttgtgca tgaagcgata ttccacggtt tgggggacat ttgggttgga aagtagtttt
     4261 ccgcgcaggt ttgccagcaa actcacatct tgtgggtgga ccttttcacg cgattgtgtg
     4321 cccacgatct ctgccgcgtc atacccaaac atgcggtatg ccgacgggct ggcaaacaag
     4381 aacattccat ctgcgtccac gagggcaata ccgtccgggg catgttcgat cagggcggtg
     4441 ttacgttttt cgatcaagcg aatggcggct tccgcttttt tacgctcggt aatgttgcgt
     4501 gagacgccat acacctccaa ccggttggtc tcagggtcaa ttacataacg tgagatgacc
     4561 tctgtccaga cggtggagcc atctttacag gtttgttcga tctcatcggt ataggatttg
     4621 gaaagaccct gttggaattt ccccagtctt tccggagtga cttgagaaag atattccaat
     4681 gaaacaggag tgagagtggc ggccatatcc tgcgtcatga cttcctccac actataaccg
     4741 cgtaaagaag tgacagaagg actgacataa cgaaagcgtg atgactccac gtccaaaacc
     4801 cagatcacat ctgaaatgtt ttcggccaat aggcggtatt tggcttcact ttcacgcatt
     4861 gcctcctcgg cttttttgcg atccgtgatt tccatgccca tgcattggat ctcgctgggt
     4921 tgattgtgtt catcggtaat gcacataaac tcccacagcg tggttcggat gccgccattg
     4981 cgcgccggtt tatcaatttc aactgaaaac acctgcccgg gttgtgaaac acattgctgc
     5041 accacgtcaa agacacgctt ccgatgatgt tcgcaaatgg tctttgtgct atcggcatga
     5101 agcaagccgt ttggttcatg aatccagcca aattcttttt cgaattgggg attccagtaa
     5161 acgtatttcc cttccatgtt aatacgtatg acgaagtggg tttgtgacgc gatcagcgat
     5221 ttaagttttt tctcgctggc caggagtgaa ttttccgcga gcttgcgttc ggtaatatct
     5281 tcaaaggttg cgtagacctg atgcggtttt tctgaatttc cgttgaagcg cggcgttgca
     5341 ttgatattga tccagttgta ggaatcaaag gctgggttgt acacccccat ggtcacattg
     5401 tgaatgggct ttcctgtttt tagggcaacc atggacgggt gggtatctcc cgggaaatca
     5461 gaaccgtctt catgaatgga tttccagcgc gggtcaatgg aggtgcgtcc ctgcatttgg
     5521 tccagtgtca atcctaatat tctttccgcg gccgggttcg cgtggatgat gcctccgttt
     5581 tcatcctgga agaccacgcc ttgcaccatc gtttcgaaca acaaacgata tttctcttca
     5641 ctttcctgta tcttttcaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 cgaaacaggt ggaacatttc ctttgaccag accattccct tgccccgttt gatatcaagt
     5821 tcccaactgc ccaaattggc tgtagccagt gccatttcca ggctggtctg acttttttgc
     5881 agcaggtcgg ttgccttttt gaggtggatt gcggtggcaa cctgcgagga aacaaattca
     5941 aggatgcgct gttcccgaat cccataagca tgagggtctg aatagttttg aactgccata
     6001 accccaatga ttttattgcg ggcgatcaat ggcaccccca gccagacctt gggttgtgtg
     6061 ccaacgacct cgatgcgctg gccggtttgg ttgtagacat acaacagtga ttctccggtg
     6121 cgcagaacat gactcgtcag ccctttgtcc ggaggcatgg attctcgtat tgggtccttt
     6181 tcgtccatcg agtagacgaa atgcagtaaa ttttcctctt catcatgcag ggcgatatag
     6241 aagttccggg cagccatgac attggatatg tggtgatgga tccccttgaa aaaatcctgc
     6301 agcgattcgg tggtttgtgc ggcattggca atgtgatata cagtttcctg taatatggcc
     6361 gattgcttgc gttctgtaat atcctgtgtg acaccgatcg cagaggcggg taccccatgg
     6421 ttgtcaaatt tgatgctcgc tttgacgcgc agccatctta tgtcatcgcc tgcaatgatg
     6481 cgatactcaa tatcgaaggg cccgccctgt agcgccgctg accatgaaac ctgtacgaat
     6541 tcccggtcat cagggtgaat gatgttcatt aactcattca gagaatgacc gcctggtgcc
     6601 caaccgatta actgggcatc ctctgatgaa gattcaaaga tgcccgtttt cagatctgcc
     6661 gtccaacttc ccaagcgagc catcaattga gactcatgca ggatctgtgt gctttttaac
     6721 aggaacactt ccgcctgctt tttctctgtg atatctcgaa tggcgctgag aattaccacc
     6781 tcgtttttca ttttgtggtg gctcaggctg atctccacgg ggaattcgct gccatccttg
     6841 cgaagtgcat agattccctg ttggatgccc gtggttttgt gctgcggatt ttgcaaatag
     6901 tagtcgcgtt tttgggggtg ggcatcacgt aatctttcgg ggatcaactg ttcaattttc
     6961 atctccagca attcgctttg tttgtagccg aacagcttct ctgtctgcag gttcgccaga
     7021 atgatcttcc cctgagcatt aacaatgatc atggcatcgg gtgcggaatc taaaaagcct
     7081 ttcagccggg cctcgctttc acttaatcgt tcttcggcca gttttcgctc tgtgatgtcg
     7141 ttcatcacgg tcaatagaaa actttggcta ttcacctgaa tatattctgc cgaaaaaatt
     7201 ccatgcctca gccctcccga ttttgtgcgg accaaaacat cttcatctct caaaaaaccc
     7261 tgctctttca tcttgcccaa taaagcccct ctttgatctg ggtcgaaaaa aagatttaat
     7321 tcaagggcgg tctttcccaa tacttcttca cgggtaaatt ccagggtttc caggaaggtc
     7381 ttattgatgt caagatattt ccccgtgttg atatcggtga tggccatttg agccgggctg
     7441 gcctggaaaa aacgggagaa acgttcttca ttttcatcag gagcgggtag tttgcgttcc
     7501 aattcctcga tccgcttttg cgccaattcc aattcctgtt gcagttgtaa tttggttttt
     7561 tgcttgctca tgagaaactc ccgtatgatt caatggtcca cctttggtta tgtaatttag
     7621 ggcaatgaaa tcgggaaggc ggatttgtac aagttgaaaa taacgcctgt cattgtaatt
     7681 gtccataagt aaaagtactt aattaaccag tgcgttacct ctgtagagta gtatagaaaa
     7741 aacccgcctc atacaaggcg ggttttggtc tttctgaatc acccctgatc attttttcag
     7801 tttcttttcc agctccatta cagtcaatac gatcactcct gtggatgcag caatcgccag
     7861 gtcgcagccg ctcagcggca ttacttcgaa gaagtgcgcc agtacgggga tatacatcac
     7921 catcaattga agaacgacca ccagcaaccc catgcctgcc agcagtgaat tgctcagcag
     7981 ccccattttg aatactgatt cttttgatga gcgtgatgcg aacgcctgaa agacctgcat
     8041 gaaagccagc gaagtgaaga tcatggtctg ccattcgggg cggcctgtga aaaagtacca
     8101 ggaaccaagc ccaagtgcga gcgcaccgat cattgcgccg acccagatgg tttgcgtacc
     8161 tgctccgcgt gagaatacgc cttcagtggg ggagtagggt ttgcgcttca tcgtatcggg
     8221 ttcggggttt tccacaccca tgccgaggcc aagcagacca tcggtgagca ggtttagcca
     8281 gagtagctgc aaaggcagca acggaagggg tttcccaaga aacggagcga tcaacatcac
     8341 cagcaccttg ccgatgttgc cagccacact gaagcgcacg aatttgcgga tattgtcgta
     8401 gatggtgcgg ccttctttga cagaggcaac gatggttgca aaattgtcat cgagcagcac
     8461 aaggtcagaa gcctctttgg aaacatccgt gccggtgatt cccatggcca caccgatatc
     8521 tgccttgcgc agggcgggcg catcgtttac accatcgccg gtcatcgaaa caatgtgacc
     8581 gttttcctgc aacgcctgca cgatgcgcag tttgtgttct ggggcaacac gggcaaatac
     8641 attgacctca tccacaacgc tccgcaattc agtggaggaa agattttcga tctcaacacc
     8701 ggtcagggcg cggccatttt ctgtgatgcc caattgccgt gcgatctcga tcgcggtcag
     8761 cggatgatcg ccggtgatca tcaccgggcg aatccctgcc gcacggcagg ttgccacggc
     8821 ttcctgcact tcgccgcgcg gcggatcgat catgccaaac agaccgacga aggtcaggtt
     8881 ttgttcaagg tcggtttgaa tgacctcagg gatggaattc atcaaacgga aggcgatgcc
     8941 caatacgcgc attccttttt tggcgagacg ttcattcgcc gcttcgatct tggacctcca
     9001 acctgaatca aggatttgag attttccttc cgaccagata tggctggtga tgtccagcaa
     9061 tccatccaca ccgcctttgg tgaaggcaat gtaccgatgg tcgccgattt ccagccctgc
     9121 aagcacggtt ggatcatatt gaccaaggtg atggacggtg gtcatgcgtt tgcgttccga
     9181 atcaaaggga agttcggcag cgcgcgggaa ggatgggtca agcgaatctt tccagtaacc
     9241 cattttggcg gcggcaacca ccagagcgcc ttcagtgggg tcacccaaag tgtggaagcg
     9301 gtcatcgcct gtgtctatca actttgagtc gttacaaagc gcgccgccga tcactgccag
     9361 cgacagagag gattgggccg gggcacccag cccacgggtg gcgcgaagcg ttccgtcgcg
     9421 ttcgatctct tcggtcagat ccagcgcatg gtcggccaca tcaagcacaa cgaccgtcat
     9481 cctgttttcg gtgagtgtgc ctgttttgtc ggaacaaatg accgtgaccg agccgagcgt
     9541 ttccacggcg ggaagtttgc gaat
//
LOCUS       DAOQLC010000144         9480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2821_length_9480_cov_30.626737, whole genome shotgun sequence.
ACCESSION   DAOQLC010000144 DAOQLC010000000
VERSION     DAOQLC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_9480_cov_30.626737"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..1297)
                     /locus_tag="PL000_18255"
     CDS             complement(71..1297)
                     /locus_tag="PL000_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90880.1"
                     /translation="MQRDPRSSVSPELKQVRLDNEALSNFQKGIIGFDVFLSSVRPSI
                     KVKTQKVYREFQDILPAYVVDEVAQEMELVLWNKAKDIRAGVWFSKFVGECAKRLLLD
                     MASRERSLLRGAQPHEVPRDAEGEPYMEPDPPDIDRQRALDNLKKLLKSTEPPEGDSK
                     AAVQERRDTNIMQTVTTSKPYLSFVDQAPEVRDHRSLRLFVLEVSEEALAAQKFSRVK
                     RLREDHPGATPEHKELIRIYKESGLTLKQFAQELKVERETLCSYLYINTKSVPEKVMA
                     RARTFAGGDIVIYQRYRTKSMSEIFEGWATQLGLDPQASPTLYVIGLILGGVSQLTLR
                     RWRNNETRPGDEALNRYDIVIRLMEGTLKQDGYRGMNLEIEKLLARYGVKESEPKRGL
                     EFYAAIFSCSLSTIIP"
     gene            complement(1239..3011)
                     /locus_tag="PL000_18260"
     CDS             complement(1239..3011)
                     /locus_tag="PL000_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90881.1"
                     /translation="MRNPFGLLFKGLALACFWWILFTGLSTVQSQPPQLAEVLPRLAS
                     WGIAVVLSGLVAWLIEINGREALQQGLEASQFWGVQVTRGRFPVLARGAGGVSDQRAV
                     VAGLKVELEKLPEEMVALAMGLLSLPLKGKGAREREIMTFVACAKSISGDFHYTGFTL
                     LGLPPADPSYDFDPADPLLVPLAIGLSRVIEEPRGKGLLQLMRATALLKEYRALAVED
                     QSAFSQVISHFDQPASLSLAKSEAGEMNLRSDRVAALLQVLLEAEKRLPQQEAAGDRG
                     GNEREPPLQTVAPLGNAPVDVGSIGVISTAVMDPELPHQEKVTEPPQDIEGADIFVAL
                     YDLLHEPYRINGAKGELRVGLLHGKHVYLMPEALAKGLMGERFSGSKPHELINRILAC
                     LAEKGVLYQVGDKKLFQVIFRGTINEEEKTNIYKGMILFEWEKIMPLLGSRGDSLFVP
                     EVMTDESLREVRNVKLAKKKPVCDGGLAEVAPETEAEVVVCPALEPDRNDMTRYQREL
                     FDQLNKLAEGNSPHVARQQHESGVVWLYVALTKVESQEVPLSVFQVGTLLKHRDSVSG
                     FQVSENAKGSTILGFSRAETGQAR"
     gene            complement(3030..3959)
                     /locus_tag="PL000_18265"
     CDS             complement(3030..3959)
                     /locus_tag="PL000_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90882.1"
                     /translation="MQLSLEQKRNMRHYAYVNGFRNAAWLTGYVEEIRENCLFISQNG
                     YKNTAVMVFFDAKTQPKGVKQGSAISVICRVQGHKQGGDRIPVLRVLGYERPKLINMP
                     PATAYQIALKHNAPASQVKPSTAAPKTVNPRLNPNSNIVHLAGLVEACVPLPSSKNPG
                     LVVLIKQHRENDQAVPVRLYGQMMSSYKSRLKRGMPIMVEGMARMRIEDVADGHGGVT
                     QTLVPFIQTSDIRIPQRGAEFLYIPGWAGEFMYETAGSTELKDMIKSMLDEDGENAST
                     ADAHEGEGVMDSAVAAATPSLSPILASPTFDDL"
     gene            4070..4510
                     /locus_tag="PL000_18270"
     CDS             4070..4510
                     /locus_tag="PL000_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC90883.1"
                     /translation="MSALQLKAESKGHKFSDACSEMGVSQTFLSSVFSAEGGPSIDSL
                     DTRTLRSIATYLDVPLAQVLFLADILAAEDFMIQRTMDQDLAAVYTLLVNDPLWSDIT
                     PPLADFTTLTPRFKFLIALLYEQYTGRSLLRKAEATGLIDKKGG"
     gene            complement(4522..4809)
                     /locus_tag="PL000_18275"
     CDS             complement(4522..4809)
                     /locus_tag="PL000_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90884.1"
                     /translation="MTYPPFAPHELRIMAMMTPTATAVATSLSQTIFRDCVPPEQASL
                     SFIEQVLAGMTAYPLSARDIALGAILEMVEEGKATRGEDIRRIILASERFR"
     gene            5285..6145
                     /locus_tag="PL000_18280"
     CDS             5285..6145
                     /locus_tag="PL000_18280"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC90885.1"
                     /translation="MLPKNTKQGLKSLFDQGTDVFSSFAELRRQVLEQKDRAALSKIY
                     GMAQHGFIDAINFLGYLYDNGEGGVAKNPRLAAKFWYTAAKQGDALALHNLGLLYWQG
                     RGLPKQEATARQLLGMASDRFITRSDIVLGLIAESHNDATNAIHYFAEASAQKHPNIA
                     ARQAMLLLKAQGPSAADQAFSMLLQAAEQGDPLAQFNLARFYGAGLGNTGNQTNPVEA
                     SYWLNILQSNPFGKPYWNLIPTSLRTYNITDLEFSHAKSSADEWIKQHPTLPVPTDYA
                     KSIYIADETM"
     gene            complement(6210..6476)
                     /locus_tag="PL000_18285"
     CDS             complement(6210..6476)
                     /locus_tag="PL000_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90886.1"
                     /translation="MRGKPDVSAFLEGGKADRAERGTPPPPTDTATTPKMPIPTVQKN
                     WRLRWDTASALKDRVEALKREGETKLTETDFVERLIRKELGLDS"
     gene            complement(6473..7108)
                     /locus_tag="PL000_18290"
     CDS             complement(6473..7108)
                     /locus_tag="PL000_18290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC90887.1"
                     /translation="MRYACVNTKGGVGKTTTAVHLAMMLARQSSNNRVLLIDGDPQTS
                     SASWAAWRREANLTPSPTTTVLGGKAIYNEGLNLAKGFDHVVVDAGGRDAAGLRSALL
                     LAERAIIPVGASNLDAAAMTDLLEVVDLAKDYNPQLDVRVLLMRVDPRTKDTSEMLEF
                     LKEQNLNVLATRICERVSFRRAIGEGTIVQELQKDKQAIEEMEHFFKEVMA"
     gene            complement(7733..8584)
                     /locus_tag="PL000_18295"
     CDS             complement(7733..8584)
                     /locus_tag="PL000_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90888.1"
                     /translation="MSGICAPVGADNAGSMVGAQVGSGNRCGHNPAAPCLTPLEPAGR
                     NLPPIITAVIDRFERYYDSPSILPALNAVNGSTRQQRSERREALSIVFHLIMKYFNLL
                     TGRIEIPTARNGFIGLTIDWIASKCNLGYKRVQRALADLEAAGIIEVKRRAERITESG
                     YRGIASIKRVSTHLFRIFGLYDEFKRQQKYKQDQQAKKVQEMVDRENPAKAQRALFLK
                     GLTNKLANGQGKRKTHFKQLQEESVAEKERERQLLMLQIKQEHPDWSLEEVRREANKQ
                     LLRGSIN"
BASE COUNT         2119 a         2639 c         2382 g         2340 t
ORIGIN      
        1 cacatttgct gttacaaacc agagtgcagg tcttaaatga tgacttacga aaaacttttt
       61 gtcgttggtt tcaggggatg atggtagaaa gtgagcagga aaaaatggcg gcgtaaaact
      121 ctagcccccg ttttggttca ctctccttga ctccatagcg ggccagaagc ttctcgatct
      181 ccaggttcat gcctcggtaa ccatcctgct tcagcgttcc ctccatgagc cggatcacga
      241 tgtcgtaacg gtttagtgct tcatcaccgg ggcgcgtctc attgtttcgc caccgccgta
      301 gggtcaactg cgaaaccccg cccaggatca ggccgatgac atacagtgtg gggctggctt
      361 gaggatcaag acctaactgc gtggcccaac cttcaaagat ctccgacatg ctcttggttc
      421 ggtagcgctg gtagatcacg atatcgccac cagcaaatgt ccgtgcccgt gccatgactt
      481 tctcgggtac ggacttggta ttgatgtaca agtatgagca aagtgtttca cgctcgacct
      541 tgagctcttg ggcaaactgc ttcaacgtca ggccgctttc tttgtagatc cggatcaact
      601 ccttatgctc cggtgtggcc cctggatggt cctcgcgaag ccgtttgaca cgggaaaact
      661 tctgggcggc aagagcctcc tcgctcacct ccagtacaaa caggcgcaaa ctgcgatggt
      721 cacgcacctc cggcgcctga tctacaaagc tcagataggg cttgcttgtc gtcactgtct
      781 gcatgatgtt ggtgtctcgc cgctcttgaa cagccgcctt ggaatcccct tcgggcggct
      841 ctgtcgattt caataatttc ttcagattat ccagggcgcg ttgacgatcg atgtcgggcg
      901 gatctggctc catatagggc tccccctccg catcccgagg gacttcatgc ggctgagccc
      961 cacgcaatag cgatcgctcc cgggatgcca tgtccagcag caagcgcttg gcgcattctc
     1021 ccacgaactt tgaaaaccag acaccggcgc ggatatcctt agccttgttc cataagacta
     1081 gctccatctc ttgcgccact tcgtcaacga catacgcggg gagtatgtcc tggaactcgc
     1141 gatagacctt ctgggttttc actttaatgg aaggcctgac cgatgacaag aatacgtcga
     1201 accctatgat tcctttttgg aaattggaca aagcttcatt atcgagcctg acctgtttca
     1261 gctcgggaga aaccgaggat cgtggatccc tttgcatttt cactcacctg aaaacccgaa
     1321 accgagtctc tatgcttcag caacgtgccc acctgaaata ccgatagggg cacttcctgg
     1381 ctttcgacct tcgtcagagc cacatacaac cagaccacac cagactcgtg ctgctgcctt
     1441 gcaacatggg ggctgttccc ctctgccagc ttgttcagct ggtcaaacaa ttccctctgg
     1501 taccgagtca tgtcattccg gtccggctcc agcgccggac aaaccacgac ctctgcctct
     1561 gtctctggtg caacctccgc caaccctcca tcacataccg gcttcttttt ggccagcttt
     1621 acattcctga cctcacgtag gctctcgtcc gtcataacct ccggaacaaa caaactgtcg
     1681 ccacggcttc ccagcagtgg catgatcttt tcccattcaa acaaaatcat tcctttgtaa
     1741 atattcgtct tctcctcctc attgattgtt cctctgaata tcacctgaaa cagcttttta
     1801 tcgccgacct gatacagaac ccctttttca gccaggcagg cgaggatgcg gttgatcaac
     1861 tcatgcggtt tactaccgct gaaccgctcc cccatgagtc ccttcgccag ggcctcaggc
     1921 atgagataaa cgtgctttcc atgcagcagc cccacccgca attccccttt ggcaccattg
     1981 atccgatagg gctcgtgcag caggtcatat agcgcgacaa aaatatctgc cccctcaatg
     2041 tcctgcggag gttccgtgac cttttcctgg tgaggcaact ctgggtccat cacagcggtg
     2101 gatatgaccc cgatactgcc aacatccaca ggcgcattcc ccagaggtgc cacagtctgc
     2161 aagggcggct cgcgctcatt cccgcctcta tcccccgctg cttcctgctg agggagacgc
     2221 ttctccgctt ccagaagaac ctggagcagg gcggcaaccc gatcgctgcg gagattcatc
     2281 tctccggctt ccgacttggc caatgacaaa gatgccggct gatcgaagtg gctgatgacc
     2341 tggctgaaag cggattgatc ttcgacagcc aatgccctgt attccttcag caaagctgtt
     2401 gcccgcataa gctggaggag tcccttgcca cgaggctctt caataactcg actcagaccg
     2461 atcgccagag gcaccaggag aggatcggcg ggatcaaaat catacgaggg atcagctggc
     2521 gggaggccga gcagggtaaa tcccgtgtag tggaagtcgc cagatatcga tttcgcgcag
     2581 gccacaaagg tcattatctc gcgctcgcgc gcgcccttcc ctttcagcgg aagtgacaag
     2641 agccccatcg ccaaggccac catctcctcc ggcagcttct ccaactcgac cttcaaccca
     2701 gccacaacgg cgcgctgatc acttacacca cccgctcctc gggccaacac cggaaatcgc
     2761 cctcgagtga cctgcactcc ccaaaactgg gaagcctcca gcccttgctg cagggcttcc
     2821 ctgccgttga tttctatgag ccacgccacc aacccggaca gcacaaccgc gatgccccag
     2881 gaagccagcc tgggaagcac ctcggccagt tggggaggct ggctctgaac ggtggaaagt
     2941 ccggtgaaca atatccacca aaagcaagcg agggccagcc ccttgaagag cagcccgaag
     3001 ggattgcgca tagtcccgct cttagtggct tacaggtcgt cgaaggtagg ggacgcgaga
     3061 attggtgaca gtgaaggggt agccgctgcc acggcggaat ccatgacacc ctccccctca
     3121 tgtgcgtctg cggttgaggc gttctctccg tcttcgtcca gcatggattt gatcatgtcc
     3181 ttgagctctg tgctgcctgc ggtttcatac atgaactcac cggcccagcc ggggatatac
     3241 aagaactcag caccacgctg gggaatccgg atgtcgctgg tctgaataaa tggcaccagc
     3301 gtctgcgtga cccctccatg cccgtccgca acgtcttcta tccgcatcct ggccatccct
     3361 tccaccatga ttggcatgcc gcgctttagg cggctcttgt aggaggacat catctgacca
     3421 tagagccgga cgggcactgc ctggtcgttt tcgcggtgct gcttgatcag gacaaccagc
     3481 cctgggtttt tcgaggacgg cagggggaca caagcttcga caaggccagc cagatggacg
     3541 atgttgctat tggggttcag acgcgggtta accgttttcg gcgcggccgt tgatggtttg
     3601 acctgggagg caggggcatt gtgcttcagt gcaatctggt aagccgtcgc cggcggcata
     3661 ttgatcaatt tcggacgctc gtatcccagg actctcagca cggggatgcg atcaccaccc
     3721 tgcttgtgtc cttgaacccg acagatcacg gaaatcgccg acccttgctt cacacccttg
     3781 ggctgtgtct ttgcatcgaa gaaaaccatc accgccgtgt tcttgtagcc attctgggag
     3841 atgaacaggc aattctccct gatttcctct acatagcctg tcagccatgc ggcgttgcgg
     3901 aatccattca cataggcgta atgccgcatg ttccgctttt gctctaaact aagttgcatc
     3961 ccgtagtatc cccgtgtgtt ttgtgccgcg tcagcttcac attactaaca agtgataata
     4021 ttccaacctg tattcggagg caagattggc gatcaaaggg ctagcgctgt tgagtgcgct
     4081 acaactcaaa gccgagagta aggggcataa attttcagac gcctgttctg aaatgggggt
     4141 ctctcaaact ttcctctcca gcgtgttctc cgcagaaggc ggcccctcca tcgatagcct
     4201 ggatacccgt actttacggt cgatagctac ctatctggat gtgccgctag cccaggtgct
     4261 gtttctggcc gacatactgg cggctgagga tttcatgatc cagcgcacga tggatcagga
     4321 tctggctgct gtttacacgc tgctggtcaa tgacccgttg tggtctgaca tcaccccgcc
     4381 tctcgcagac ttcaccacgt tgacaccgcg cttcaagttt ttgatcgccc tgctctatga
     4441 acagtacaca ggcaggtccc tgttacgaaa agccgaggct acaggcctca tcgacaaaaa
     4501 agggggctaa tgcccctctt ttcaccggaa ccgctcggat gcaaggatga tccgtcgaat
     4561 gtcttcccct ctggttgcct tcccttcctc taccatttcc aggatcgccc ccagggcgat
     4621 gtctcgcgca ctgaggggat aggccgtcat tccggctaaa acctgctcga taaagctcag
     4681 gctcgcttgt tcgggcggaa cgcagtcgcg aaaaattgtc tgtgaaaggc tggtggctac
     4741 ggctgttgcg gtcggcgtca tcatcgccat gatcctcaat tcatgtggtg caaagggggg
     4801 ataggtcatg gttaagtgcc ctggctcccc ccggataacg ccaaagctca aatacatatt
     4861 gcacgccata gatagctagc tatatagcta gctatctatg gcgaaagaag tcttgaagaa
     4921 cgtgaccgtc cccggaaacg caccctgtcc agggttggga ttttgcagga tctgcaggta
     4981 tgagacctgt gttcatccca tgataaagcc gttccaccac gatcattaaa aagcatttca
     5041 caagctagta atatgtgctc taggtcacat ctccattaac ccgttcactt attgttggct
     5101 tattgctttc cctcttgatg acactatttc atgatatgat ttttagtcaa agcggcgcta
     5161 agaaggcaaa gctcgcacta cttcatgagg gaaatgacga atgattccgg caagattgag
     5221 gcgtggtttt gttgcaattt gtatctgctt atcccctttg gccagggctg atctggatat
     5281 ccccatgctg ccaaagaaca ccaagcaggg cctgaaaagc ctgtttgacc agggaactga
     5341 tgttttttcg tcctttgcag agctacggcg ccaagtcttg gaacaaaagg atcgagcagc
     5401 gttgtcgaaa atctatggca tggcccagca tggatttatc gacgccatca acttcctggg
     5461 ttacctctat gacaacgggg aaggcggcgt ggcaaaaaac ccgcgcctgg ctgcgaaatt
     5521 ctggtacacc gccgccaagc agggagatgc cctggcattg cataacctcg gtcttcttta
     5581 ctggcagggg cgaggactac caaaacaaga ggccacggca cgccagttgc ttggcatggc
     5641 ctcggaccgt ttcatcactc gatcggatat cgtgctgggt ctgattgcgg aatcgcataa
     5701 tgacgcaact aacgctattc attatttcgc ggaggcgtct gcgcaaaagc accccaacat
     5761 agcagcccga caggcgatgc ttttgctcaa ggcgcaagga ccatcagcag cggatcaggc
     5821 attctccatg cttcttcaag ccgcagaaca gggcgaccca cttgcccaat tcaatttggc
     5881 caggttctac ggggccggac ttggcaacac tggcaaccag acgaatccgg ttgaagcatc
     5941 gtattggcta aatattctcc agtccaaccc gttcgggaaa ccttactgga acctgattcc
     6001 tacttcactt cggacctaca acatcaccga tctagagttt tctcacgcca aatcctccgc
     6061 agatgaatgg atcaagcagc atcccacatt gcccgtaccc acagattacg caaaaagcat
     6121 ctatattgcg gatgaaacca tgtagctagc tatatagcta gctacattct ttagccacca
     6181 tcagtcatta ctgctagcta gctatataac tagctatcta gcccgagctc tttcctaatc
     6241 agccgctcca caaaatccgt ttctgtcagt tttgtttctc cttctcgttt tagtgcttca
     6301 actctgtctt taagggcaga ggcggtatcc cagcggagac gccaattttt ctgaacagtc
     6361 ggaatgggca ttttcggtgt agttgccgta tccgtgggcg gcggcggagt tccacgttca
     6421 gctctatcgg ccttgccccc ttctaaaaat gcactgacat caggcttgcc tctcatgcca
     6481 tcacctcttt aaaaaaatgt tccatctcct cgattgcttg tttgtctttc tgcaattcct
     6541 ggacgatagt tccctcaccg atagcccttc gaaaggagac acgctcacag attctagtgg
     6601 ccaagacgtt caagttttgc tccttcagaa actccagcat ttcagatgta tctttggttc
     6661 gtggatccac gcgcatcagt aggacacgaa catccaattg aggattgtag tccttggcca
     6721 ggtcgaccac ttccagcagg tctgtcatcg ccgctgcatc cagattcgag gctccgacag
     6781 gaataatcgc acgttcggcc agcagaagag ccgagcgtaa tccggccgcg tcgcgaccac
     6841 ctgcgtcgac gacaacatgg tcaaaacctt tggccagatt caatccttcg ttgtagatgg
     6901 cttttccgcc tagaactgtc gtggtcggtg aaggagtgag gttggcctcg cgtcgccatg
     6961 ctgcccaact ggcggaactg gtttggggat cgccatcgat gagaaggaca cggttatttg
     7021 aagactgcct ggccagcatc attgccaggt gtacggctgt agttgtttta ccaacaccgc
     7081 ctttcgtatt tacacaagca tatcgcatag atttgttccc tttcctgcta gctatatagc
     7141 tagctagcag tatagctagc tatatagcac aagtcaatta aagatggtat aaggaacgag
     7201 ctgatgcgga ggttggtcca atagcgatat ttgcagatcg gtcatcaaga cgaattgttt
     7261 actgaagtgc ggagagaggc ttgtgccaga ttcgacgcct tattcctaaa aacaaaaaat
     7321 tcgggtgaaa gtcgcgccag aggcgcggtc aatggatgac ccgttagggt catttaaggc
     7381 gcgagttgtc ggtagagctt gagaagatgg tgttcgcgcc gtaacgagcc gtaggctcga
     7441 tattttatga agaaggatgt gaagggcgcc cgcgagggcg cttcatttca atgaagcata
     7501 gatatagata aatccttatt tgagtatgga agaccactta aggattgaaa ctaggacact
     7561 taaattcagt gtcagctcta tggccggatg ggcttttcgt gagttcctga ttgcgcgttg
     7621 cttttacggc atccgctggg gcgttctgac gacttttggc ggagcggaag agggctagtg
     7681 ggtgtatttg ggaggcgtgc ccacgggggg tctcgggagg ttcccgattc gattagttga
     7741 ttgagccgcg gaggagttgc ttgttggctt ctcgtctgac ttcttccaac gaccagtctg
     7801 gatgctcctg cttgatctgc aacatcagca gttgccgctc tcgctccttc tcggctacag
     7861 actcttcctg aagctgcttg aagtgtgtct ttcgctttcc ctggccattc gccagcttat
     7921 tggttagccc cttcagaaag agggctctct gggccttggc agggttttcc cgatcaacca
     7981 tttcctggac tttttttgcc tgctggtcct gtttgtattt ctgctgtctc ttgaattcgt
     8041 cgtagaggcc gaaaatccgg aaaaggtgcg tggaaacacg tttgattgag gcgataccac
     8101 ggtagccgct ttcggtgatc cgctctgctc tgcgcttcac ctcgatgatg ccagcggcct
     8161 ctaggtctgc caaggcgcgt tgcacacgct tgtagcccag attgcacttg ctggcaatcc
     8221 aatcgatagt tagcccaatg aagccgttcc tggcggtagg gatctcgatg cgccctgtca
     8281 gaagattgaa atacttcatg atcaagtgga agacaatgga caaagcctca cgccgttcac
     8341 ttcgctgttg gcgcgtgctg ccattgacgg cattcaatgc cggcaggata gacggagagt
     8401 cgtagtaacg ttcaaagcga tcgataaccg ccgtgatgat cggcgggaga tttcgcccgg
     8461 ccggctccag gggagttaga catggggcgg ccggattgtg tccacaccgg ttaccgctac
     8521 ccacctgcgc tccaaccatc gaaccggcat tgtcagcccc aactggggcg caaatgccac
     8581 tcatagcaat tccttgtctt gcttttgagt gatccgatgg ctagaatgtg gagactaatc
     8641 cgaagacggg tctcctctgg aggcgtttaa agttgtcggt cgccaaacta atcaactttc
     8701 tacgctttgg tgttctagct cttcggatca cggccctcga ttgagggcct taattttttg
     8761 tacgtctgaa aacttcttcc cagtccgcaa ctaaattctg atccttaccg tttttctctt
     8821 cgcagcctag ctccggccaa ctcatcatgt agtgagcact ggcgtcggga agcgcaggga
     8881 aaacgaatta ttcttgaaac agctgcatat tactactatg caaggtttcc ttgcaacatg
     8941 aattcgaatt cagaattgaa ttcaaatcat gccatggttc cggaggatac gacttgttgg
     9001 acacttctgc aacaggtgga catctgcata gaaatcatca tggtccaagc agcaagtcca
     9061 tcaaccgagt gatgtgttgt ctcttcctcc agtaaaaatc tacatcatgt ttgtcaacgt
     9121 agaatcccgt aggctcctcg gaagagaaat cgagaaagta gagctcagct cggctttggc
     9181 ctgatggctt catggcaatt ccatctgcga ggataccgta ttccgcgcgg ctgccgccca
     9241 aggaggttga gccaaacatc cgagcaaaaa tgcgaaactt ggaagtgcca agatcgcgct
     9301 gattgccgcc gacgatcaac cttacttgtt ccgatagtat gcctcctcct ccgtacgtga
     9361 gggtccaacg ggattctgaa tttttttcac aaaaagtgat ttaaagtgtt gatattatta
     9421 atatagggta tacggattta atggttgatg gggcaccttc accccatcaa tctgatacca
//
LOCUS       DAOQLC010000145         9421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2845_length_9421_cov_18.297352, whole genome shotgun sequence.
ACCESSION   DAOQLC010000145 DAOQLC010000000
VERSION     DAOQLC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_9421_cov_18.297352"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PL000_18300"
     CDS             complement(<1..322)
                     /locus_tag="PL000_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC90889.1"
                     /translation="MTNIQKTMLKAVEEELQRQVARLDQPRTLPFHEMLTYHMGWTGD
                     GAGPEATGKRIRPLLVLLSVASCEETTTTTSTSTSTSAPSWLRAKSAAAAVELVHNFS
                     LVHDD"
     gene            complement(366..968)
                     /locus_tag="PL000_18305"
     CDS             complement(366..968)
                     /locus_tag="PL000_18305"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNC90890.1"
                     /translation="MDSAITTFIFDFGNVFVKWDIHALYNRFFPNPQAVDSFLEEIRF
                     HEWNAHQDAGRPFKDGIAILSEKYPHYADLIQAYDTHWEDSITHMYHGTVEILRRLKG
                     EGWPIYLLSNFSAEKFPLMRERYEFLNLFDDMIISGEHKLIKPDPAIFQLTLERIDRK
                     ASECLFIDDSLANIETARNMGFHTIHFQSPEQLERDLKIW"
     gene            complement(969..1895)
                     /locus_tag="PL000_18310"
     CDS             complement(969..1895)
                     /locus_tag="PL000_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90891.1"
                     /translation="MRSSDIKYLSFGLGIGALVASLFFIGFLFIPQSNPPADTLIAGI
                     KNTLVPATATPQNTETSTPAPTAEGTLTPTFTNTPTAQPTSTPLPTATATLSTAQIMI
                     TTGEIVPAGPLSLEQQIKLYEASLFFIAPTYEQSKQMSVLINHVRFSDPSNTCGPLSI
                     AILQKAGIISETTLEAHDFFLINPDLGKDQITIKTAFPSERYTNTRYKVKINQFDWRA
                     NPLLPGDFVYIYSGTEGNFEHMLVVNRVDSQGRVYSVTNYNTEQGFVITEVMLYDPSD
                     PTLGMFAQWTAKPRAKLGSTGFAGFEVWRLKQ"
     gene            complement(1941..2792)
                     /locus_tag="PL000_18315"
     CDS             complement(1941..2792)
                     /locus_tag="PL000_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HNC90892.1"
                     /translation="MKKAFCALLITLLLAGCNTSQVRGPAPLEAGYRYSSYGPTSNPG
                     AEYWPEVGQRIASEFENATPATIWIVGVVDGEGSYLNFPCETDDPNIHCGPVDMNESM
                     LTTFDEQGFDVWLQVEAGNADMDELIDIVLGQYEHHPSVVGFGVDVEWYKSSQVPQGE
                     PVSDEVAQAWVRAIRNHHPEYQLFLKHWDTAWMPPTARDGILFINDSQGFESFEQMTT
                     EFAEWGKTFAPARVGFQYGYQADQRWWSEMQNPASEIGHVLLEKIPNTQALFWVDFTI
                     LETFPPE"
     gene            complement(2789..3586)
                     /locus_tag="PL000_18320"
     CDS             complement(2789..3586)
                     /locus_tag="PL000_18320"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC90893.1"
                     /translation="MRSIGEYLRLYFIIEAQYIKARMQYRADFIISSIGMFFSSLVTL
                     GVFWVIFDTVPDLAGWSLMEMAFIYAFYMIAISPMQVIFDHFWQLRFNLQQGNFLKYY
                     FRPLNMMFYYTSEMFDLKGLTQFFVGIALLVYTANQLHVTWTFARVVLLFVTLFSAAL
                     VQISIVVLAACSAFWILDSYPVLGLAWRLREFAPYPMTIFDGAFRFTFTYILPIGFIA
                     FYPSQIFLRPEDVSTLTYFSPIVGIGLFAFTYWVWKKGVNSYTGTGS"
     gene            complement(3589..4380)
                     /locus_tag="PL000_18325"
     CDS             complement(3589..4380)
                     /locus_tag="PL000_18325"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNC90894.1"
                     /translation="MRKYLAFTRGSFMVGLVYRFGPLLALIGNFVYLGVAYYLWHSIY
                     KNSDTLRSMTFNETFLYVAIGSAVFILLKTYVDWEVSYEIREGIISTYLTKPLDYQTY
                     ILFTSLGQTLMSLMVITLPTVLVVTLVFKVSIPLGIGTLLFPFSLLLAFVISFCFDYF
                     TGILAFYTESVWGLSTTKEIIILALSGALIPLQFFPAKLQSILLLLPFQTIYHTPLMM
                     LTRPNQDVRTFVGMLIVQGVWAVVLYVLTRLFFEQAVKVLRVAGG"
     gene            complement(4377..5399)
                     /locus_tag="PL000_18330"
     CDS             complement(4377..5399)
                     /locus_tag="PL000_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC90895.1"
                     /translation="MSLIHVNNLTKHFKILNRREGLSGAFRDLFSGDYRTVKAVDGIS
                     FDIEAGEIVGYIGPNGAGKSTTIKMMTGILKPSSGEIRINNHIPFENRIRQAQIMGVV
                     FGQRTQLWWDLPVIESFRILKEIYKVPQKTFDEHMGMFNELVGLKALYSQQVRTLSLG
                     QRMLCDITASFLHNPQIVFLDEPTIGLDISIKAKIRTVIKELNEARKTTIILTTHDLG
                     DVEALSQRVIIIDKGKKLYDGDTKKVNALFGAYRTLKVQVNDFNEGTLDALKNKLAAQ
                     FGATNKIVIAQTEEFWTDITIDQAATPLSEVLNYVMTNFSILDVRIVEIAMEDVVQRI
                     YDGALS"
     gene            5579..6664
                     /locus_tag="PL000_18335"
     CDS             5579..6664
                     /locus_tag="PL000_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNC90896.1"
                     /translation="MITVQNLSKNFGELVVLKDINVAINKGEVISIIGPSGTGKSTFL
                     RCLNLLEKPNHGRIVIDGVDILKKGVNVPKLRQRMGMVFQSFNLYEHLTVLGNLTIGP
                     IKLRGMSKQAAEEKAMELLKTVGLSEKADSYPDELSGGQKQRVAIARCLAMDPEIILF
                     DEPTSALDPTMISEVLAVIRRLAKGGMTMAIVTHEMDFARDVSTRVFYMDEGTIYEEG
                     SPMDIFENPQKDKTRAFVRQLRVLRQEISSLNFDLYKLNASIETFCAKHTIDKRVVGD
                     ILLLVEELLVNMLLPVKQEGQIINLALEYAEKRDEFQIVFDYPGESYDPTFDDSADNL
                     ALMIVKKKARDIRHLYVNGLNQLSVSI"
     gene            6677..6991
                     /locus_tag="PL000_18340"
     CDS             6677..6991
                     /locus_tag="PL000_18340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC90897.1"
                     /translation="MIHKTINENDVTLAILGRLDSVTQSELAQELETVFKTKVYTLIL
                     DLSGLEYLSSAGLRVFLSARRMASETGGKVKIIGASDAINEILQITGYADITVMAKRG
                     RS"
     gene            6988..8517
                     /locus_tag="PL000_18345"
     CDS             6988..8517
                     /locus_tag="PL000_18345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HNC90898.1"
                     /translation="MSNDHIRKIAGFFAVLTIFSLLLVACAPSAVSEAAQGTTMDEVA
                     KGRVGVLLGTVYDTYLVKNYPSTQVLQYKNYPDLILAVQSGKVDSGFINCQAFKELQK
                     ENPFLTLLVGDVFSNPVGVGFSQENDPLRGEFNKFLTEIKSNGVYDDWHRRWFQDGNY
                     EMPVIENTKANGEVVIGIVSDKGYPFSVIKDGQLVGSDIELAERFGAYLQKEVVFSDM
                     EFGSLIAAVSTDKVELIVSTIMITDERKKQIDFSDPYFELSACVMGFAPEQAETAKTT
                     SAFSSISKSFYNNIIVENRYLLILDGVKTTGLISLLAILLGTLLGALICFMRMSHQKF
                     MQGFARVYISIIRGIPVLVLLMLIFYVVFARVNVDPILAAVLAFGINFAAYVSEMFRA
                     SVESIDKGQTEAGVAGGFTKAQTFFYIVMPQAARRVLPVYKGELISLVKMTSIVGYIA
                     VQDLTKASDIIRSRTFDAFFPLIMTAVLYFFISWLLLVLMSAVERKIEFRRRSTVAES
                     N"
     gene            complement(8554..9294)
                     /locus_tag="PL000_18350"
     CDS             complement(8554..9294)
                     /locus_tag="PL000_18350"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNC90899.1"
                     /translation="MGNSIKKVGGLGMFHTPPIHRLEKFMIPSTANIACDSFVESLLR
                     LLSLRDIETEEHTRRVAALSLDLARAYGKFSHAELFDIYQGALIHDVGKIGIPDAVLQ
                     KRGSLTPEEEAQIRMHPIYAYELFRTLPCFEPILDIAYCHHEKWDGSGYPRGLRGEQI
                     PLMARLFTIADVWDALTSERPYRAPWPEEKVHDYILEQSGRYFDPAIVKLFISRYFRR
                     STPRKSAKKFIGYTTLQQKVNLFIGEGR"
BASE COUNT         2407 a         2032 c         2518 g         2464 t
ORIGIN      
        1 tatcatcatg aactaatgaa aagttatgga cgagttccac cgcggctgcg gcagactttg
       61 cgcgtagcca agaaggggcg gacgtagatg tagatgtaga tgtagtagtg gtggtttctt
      121 cacaggaagc aacagagagt aatacaagca agggacgaat tcttttccct gtagcttccg
      181 ggccggcgcc atctcccgtc cagcccatgt gataggtgag catctcatgg aagggcaggg
      241 tacgcggctg atcgaggcgt gcaacctgcc gttgtaattc ttcttcaaca gccttgagca
      301 tggttttttg aatatttgtc atggtggtgt tccgttttgc agagttggtt ttggctcgca
      361 cgactttacc agattttcaa atccctttcc agttgttctg gcgattggaa atgaatggtg
      421 tgaaagccca tgtttcgtgc ggtttcgatg tttgccagag aatcgtcaat gaacagacat
      481 tcactggctt tgcgatcgat cctctccagg gtgagttgga agatggcagg atctggtttg
      541 atgagtttat gctcgcctga gatgatcatg tcatcgaaca ggttgagaaa ctcgtaacgt
      601 tcccgcatca gcgggaattt ttcggcggaa aaattactga gcaggtaaat tggccagcct
      661 tcccctttca acctgcggag aatttccact gtgccgtgat acatgtgggt gatgctgtct
      721 tcccagtgtg tgtcataggc ttgaatcaga tcggcgtaat gcggatactt ttccgaaaga
      781 atggcaatcc catccttgaa tggacggcct gcgtcctgat gggcgttcca ttcgtggaag
      841 cgaatttcct cgagaaaaga gtcaacagct tgcgggttgg gaaagaagcg attgtaaagg
      901 gcgtggatat cccacttaac gaagacgtta ccaaagtcga aaatgaaggt cgtgatggcg
      961 gaatccattt attgttttaa tctccagacc tcaaaacctg caaacccagt agagccgagt
     1021 tttgcgcgcg gttttgccgt ccattgtgca aacatgccga gcgttgggtc cgatgggtca
     1081 tacagcatga cctcagtgat gacgaaaccc tgctccgtgt tgtagttggt aaccgaataa
     1141 acgcgcccct gcgaatcgac acgattgacg acgagcatgt gttcaaaatt gccctcggtg
     1201 cctgagtaga tgtaaacgaa atcaccaggc aggagcgggt tggcacgcca atcgaattga
     1261 ttgatcttta ctttgtatct ggtgtttgtg taacgctcac tcggaaacgc ggttttgatt
     1321 gtgatctggt ctttgccgag atcaggattg atcaggaaga agtcgtgtgc ttccagcgtg
     1381 gtttcgctga tgatgcctgc tttttgcaaa atggcaattg aaagcgggcc gcaggtgttg
     1441 cttgggtctg aaaagcggac atggttgatc agcaccgaca tttgtttgct ttgttcgtag
     1501 gtcggtgcaa tgaagaacaa ggatgcttca tataacttga tctgctgttc gaggctgagc
     1561 gggccagcgg ggacgatctc tcccgttgtg atcatgatct gcgcggtgga aagtgtggcg
     1621 gttgccgttg ggagcggcgt tgaagtgggt tgagcagttg gggtatttgt aaatgtgggc
     1681 gtaagtgtgc cttctgctgt gggggcagga gtggatgttt ccgtgttttg cggcgttgct
     1741 gtggcaggga ccaaggtgtt cttaataccc gctatgaggg tatcggcagg tgggttcgat
     1801 tgcggaataa aaagaaaccc aataaaaaaa aggctcgcta ccagcgcgcc aattcccaac
     1861 ccaaaactta gatatttgat gtcagatgat cgcatgtcag tttcaatatt attattttaa
     1921 tcgaaccttg gaatcaatgc tcattctggc ggaaaggttt caagaatggt gaaatccacc
     1981 cagaaaagcg cctgtgtgtt cggtattttt tcaagaagca catgcccgat ctcggaagca
     2041 gggttttgca tttcactcca ccaccgttga tcggcctggt agccatactg aaaaccgacg
     2101 cgggcagggg cgaaggtctt tccccattct gcaaactcgg ttgtcatttg ctcaaaagat
     2161 tcgaagccct gactgtcgtt gatgaacaga atcccatcgc gggctgtggg cggcatccag
     2221 gcggtatccc aatgcttgag aaagagttga tattctggat gatgatttcg gatcgccctt
     2281 acccatgcct gagcaacttc gtctgatact ggttcgccct gtggcacttg cgacgatttg
     2341 taccattcca catccacccc gaaaccgacg acgcttggat gatgttcgta ttgaccaagg
     2401 acaatatcga tcagttcgtc catgtcggcg ttgcccgctt ccacttgcag ccatacatca
     2461 aatccctgct cgtcgaaggt ggtaagcatg gattcgttca tatccactgg gccgcagtga
     2521 atgtttgggt catctgtttc gcaagggaaa tttagataac tgccttcgcc atccacgacc
     2581 cctacgatcc agatcgtggc gggggttgcg ttctcgaatt ccgatgcgat gcgttgacca
     2641 acctcgggcc aatactccgc cccagggttg gatgttggcc cgtaggatga gtagcggtat
     2701 cccgcttcca atggggcggg gccacgtact tgcgaggtgt tgcagcccgc cagcagcagc
     2761 gtgatgagga gtgcgcagaa tgccttcttc atgacccagt ccctgtgtag ctgttgacgc
     2821 cctttttcca tacccaatag gtgaatgcaa atagcccgat gccaacaatg ggcgagaagt
     2881 aggtcagcgt ggaaacatcc tctggcctca aaaagatctg tgatggataa aaggcgatga
     2941 atccgattgg caggatgtag gtgaaggtga aacgaaatgc accgtcgaag atcgtcatgg
     3001 ggtagggggc aaactcacgc aagcgccagg ccagtcccag cacggggtag gagtctaata
     3061 tccaaaaagc ggagcaggca gccagcacca cgatggaaat ttgtaccagt gcggcgctga
     3121 acaaggtgac aaacagcaga accacccgcg caaaggtcca tgtgacatga agctgattgg
     3181 ctgtgtagac cagtaaagcg atgcccacaa agaattgggt cagccccttc aggtcgaaca
     3241 tttcggaggt gtagtagaac atcatgttca acggacggaa gtaatacttc agaaaatttc
     3301 cctgctgcaa attgaagcgc agctgccaga agtggtcgaa gatgacttgc atgggcgaga
     3361 tggcgatcat gtaaaacgcg tagatgaaag ccatttccat caaagaccag cctgcaaggt
     3421 cgggtacggt gtcgaagatg acccaaaaga cccccagtgt gaccaggctt gaaaagaaca
     3481 ttccgatcga gctgataatg aaatctgcac gatattgcat gcgcgccttg atgtattgcg
     3541 cctcaatgat gaagtacagc cgcagatatt cgccaatgct gcgcatggtt agcctcccgc
     3601 gactcgcaag actttgaccg cctgttcgaa gaagagacgg gtcaaaacgt acaggacaac
     3661 tgcccataca ccttgaacga tcaacatgcc gacaaatgtc cgcacatcct ggttgggtct
     3721 ggtcagcatc attaatgggg tatggtaaat cgtttgaaag ggcagcagca acaaaatgct
     3781 ttgcagtttc gcagggaaga attgcagtgg aatcaaggcg ccagaaagcg caaggatgat
     3841 gatctccttg gtggtgctca gcccccaaac cgactcggtg tagaacgcga ggatgcctgt
     3901 gaaatagtcg aagcagaaac tgatgacgaa tgccagaagc aggctaaagg gaaagagaag
     3961 cgttccaatc cccagtggaa tactcacctt gaaaaccaat gtcacaacga gcacagtcgg
     4021 cagggtgatc accatcagac tcatcagcgt ttgaccgagg ctggtaaaaa gaatgtaggt
     4081 ctgataatcc agcggtttgg tgaggtaggt ggagatgatg ccttcgcgga tctcgtatga
     4141 gacttcccaa tccacgtagg ttttgagcag gataaaaact gcggagccaa tggcaacata
     4201 taaaaaagtt tcgttgaagg tcatgctgcg cagtgtgtcg gagtttttgt aaatgctgtg
     4261 ccagagatag tacgcgacgc cgaggtaaac aaagtttccg atcagcgcaa ggagcggacc
     4321 aaagcgataa acaagcccaa ccatgaagga gccgcgtgtg aatgccagat actttctcat
     4381 gacagggctc catcataaat gcgttgaacg acatcctcca tggcgatttc cacgatgcgg
     4441 acatccagaa tggagaagtt ggtcatcaca tagttcaata cttccgaaag cggagtggcg
     4501 gcctggtcaa tggtgatgtc tgtccagaat tcttcggttt gggcaatgac gatcttgttg
     4561 gttgcgccga actgtgcggc gagtttgttt ttgagggcat cgagcgtgcc ttcgttgaaa
     4621 tcgttgacct gtactttcag cgtgcgatac gctccaaaca gggcattgac cttctttgtg
     4681 tcgccatcgt acagcttttt gcccttgtca ataatgatga cacgctgcga taaggcttcg
     4741 acatcgccaa ggtcatgcgt ggtgaggatg atggtggtct tgcgcgcttc gttcagttct
     4801 ttgatgacgg tacggatctt ggccttgatg gaaatatcca gcccgatggt cggctcatcc
     4861 agaaatacga tctgtgggtt gtgcaggaaa gaggcggtga tatcgcacaa catgcgctga
     4921 ccgagcgaga gtgtgcgcac ctgctgcgaa tacaaggcct tcaacccgac cagttcattg
     4981 aacatgccca tatgctcgtc aaaggttttt tgcggaacct tgtagatttc tttcaatatc
     5041 ctgaacgatt caatgacggg caggtcccac cacaattgcg tgcgttgacc aaagaccact
     5101 cccatgatct gcgcctgacg gatccgattt tcaaacggga tgtggttgtt gatgcggatc
     5161 tcgccgcttg aaggtttgag aatccctgtc atcattttga tggtggtgga tttgcccgcg
     5221 ccgtttggtc caatgtaccc aacgatctca cctgcttcaa tatcaaatga gatgccgtcc
     5281 actgctttga cggtacgata atcccccgaa aacaggtcgc gaaatgcgcc gcttaaacct
     5341 tcgcggcggt taagtatttt gaaatgtttg gtcaggttgt tgacatgaat gaggctcatg
     5401 aactggattt tacaccgtaa tctggcggag gctcatgagg ttggtagagg actttgctcc
     5461 atttcggctc ttaaatctgg gtgctataat cagtatgttc catactgggg aatactgttt
     5521 ggaaagaaag atttcaagaa agcctattct atcggcgatg gcgcgttgag gtgaaaccat
     5581 gatcactgtg caaaatcttt ccaagaattt tggggaactt gttgtactca aagacattaa
     5641 tgtagcaatc aacaaaggcg aagtcatatc gattatcggc ccatctggta ctggtaaaag
     5701 tactttcctg cgttgtctta acttgctcga aaagccaaat cacggaagga tcgtgatcga
     5761 tggcgtggat attctaaaaa agggagtgaa tgttcccaag ttgcgccaaa ggatggggat
     5821 ggttttccag tcttttaatt tatatgagca tctgaccgtg ttgggaaact taaccattgg
     5881 cccgatcaaa ttgcggggaa tgagtaaaca ggccgcagaa gaaaaggcca tggaactact
     5941 caaaacagtg ggactctctg aaaaagcgga tagttatcct gatgaacttt caggtggaca
     6001 aaaacaacga gtcgccatcg cccgatgtct ggccatggac cccgagatca ttctcttcga
     6061 cgaaccaact tcagcccttg acccgacaat gattagtgag gttttggctg tgattcgtcg
     6121 tctagccaaa ggcggaatga cgatggcgat cgtgacacat gaaatggatt ttgcccgaga
     6181 tgtttcaacc agggtgttct acatggatga aggaacaatc tatgaagagg gaagcccgat
     6241 ggatattttt gagaatccac aaaaagataa aacacgtgca tttgtccgtc aactgcgtgt
     6301 tttgaggcag gaaatcagca gcctgaattt cgatttatat aagttaaatg ccagtataga
     6361 gactttttgc gccaaacaca ccattgataa gagagtggtt ggtgacattc tgctgcttgt
     6421 ggaagagttg ttggtcaata tgttgctgcc ggttaaacag gagggacaaa tcattaacct
     6481 ggcgttggaa tatgctgaaa aacgtgatga atttcagatt gtattcgatt accccggcga
     6541 atcatatgac ccaacatttg atgatagcgc agataacctt gcgctgatga ttgtaaaaaa
     6601 gaaggccagg gatattcgtc acttgtatgt aaatggtctg aatcaacttt ccgtttcaat
     6661 atagaggctt cccatgatga tccataaaac cataaatgaa aatgatgtga cccttgccat
     6721 tttaggcaga cttgatagcg taacacaatc tgaattggcg caggagttgg agacggtatt
     6781 caaaacaaaa gtatatacgt tgatattgga tctttcgggt ttggaatatc tcagcagtgc
     6841 tggcctgcgg gtttttctat ctgcacgaag aatggccagt gagacgggtg gaaaggtaaa
     6901 aatcatagga gcaagtgatg caattaatga gattcttcag atcaccgggt atgcagatat
     6961 cacggtgatg gcaaagcgag gcaggtcatg agcaacgatc atattcgaaa aattgctggc
     7021 ttttttgcgg ttttgacaat attctcactt ttacttgttg catgtgcccc gagcgcagtt
     7081 tctgaagcgg cccaagggac taccatggat gaagttgcaa aaggcagggt gggggtgctg
     7141 cttggcaccg tttatgatac atatttggtg aagaattatc caagcacgca ggtcttgcaa
     7201 tataagaatt atcctgactt gattctggct gttcaaagcg gtaaagtcga ttcgggattt
     7261 atcaactgcc aggcatttaa agaactgcaa aaagaaaatc cgtttctgac acttcttgta
     7321 ggtgatgttt ttagtaaccc tgtcggtgtt gggtttagcc aggagaatga cccgctacga
     7381 ggagaattca ataaatttct tacagagatc aaatctaatg gcgtatacga cgactggcat
     7441 cgacgctggt ttcaggacgg caattatgaa atgcctgtaa tcgagaatac aaaagccaat
     7501 ggtgaggttg tgatcggcat tgtgagcgac aaaggatacc ctttctcggt gatcaaagac
     7561 ggacaattgg taggttcaga cattgagctg gctgaacgtt tcggtgcata ccttcaaaaa
     7621 gaggtcgtgt tttctgatat ggagttcggc agcctgattg ctgccgtttc cactgataaa
     7681 gtggaattga tcgttagtac gatcatgatc actgacgaaa gaaagaagca gattgatttc
     7741 tctgatccgt attttgaatt aagcgcctgt gtgatggggt ttgcgccaga acaggcagag
     7801 accgcgaaaa ccacttccgc tttttcttcc atttcaaaaa gtttttacaa taacatcatt
     7861 gtggaaaatc gttacctttt gattttggat ggtgtgaaaa ctactggatt gatctcgttg
     7921 ttggcaatcc tgctcggcac actgttggga gccttgatct gttttatgcg catgtcgcat
     7981 caaaaattta tgcagggatt tgccagagtt tatatttcca ttatccgagg gattcctgtg
     8041 ttggttctgt tgatgctgat tttctatgtg gtctttgcta gagttaatgt tgaccccatt
     8101 ctagcggcag tgttggcgtt tggtattaac tttgcggcct atgtgtcaga gatgtttcgc
     8161 gcctctgttg aaagcattga caaggggcaa actgaggcag gtgttgcagg tgggtttaca
     8221 aaggctcaaa cattcttcta tattgtgatg ccgcaggctg cgcggcgggt tttgccagtt
     8281 tacaaaggtg agttgatttc gctggtgaaa atgacctcta ttgtcgggta tattgctgtt
     8341 caagacctga ccaaggccag cgatattatc cgaagccgta cgtttgacgc ctttttccca
     8401 ttaatcatga ctgcagtgtt gtattttttc atatcatggc tgttactggt tttgatgagt
     8461 gctgtggaaa gaaaaataga gtttagacgc cgcagcacag ttgcagaatc aaattagtgg
     8521 agggaaaagt aaattcgctc taatcctgcc ttttcacctt ccttcaccaa tgaataaatt
     8581 gaccttctgc tgcaatgtgg tatatccgat gaatttttta gcggatttgc gtggggtaga
     8641 tcggcgaaag tagcggctga taaacagctt gacgatggca gggtcaaagt acctgcctga
     8701 ttgttcgagg atatagtcat gtactttttc ttcgggccag ggtgcgcggt aggggcgttc
     8761 cgaggtgagg gcgtcccaca catccgcaat ggtgaagagg cgggccatca gcggaatttg
     8821 ttcgccccgc aagcctcgtg gatagccgct accatcccac ttctcgtgat ggcagtaggc
     8881 aatgtcgaga atcggctcaa agcatggcag ggtgcggaac agttcatagg cataaattgg
     8941 atgcatgcgg atctgcgcct cctcctcagg ggtgagggat cccctttttt gcagaacggc
     9001 atcgggtatc cctatttttc ccacatcatg gatcaaagcg ccctgataga tatcaaacaa
     9061 ctcggcatga ctaaatttcc cataagccct tgcaaggtca agtgagaggg cggcgacacg
     9121 gcgggtatgt tcttcagttt cgatatcacg tagtgagagc agccgtaaca ggctttcgac
     9181 gaatgagtcg caggctatat ttgccgtact tggaatcatg aatttttcca atctatgtat
     9241 gggaggggtg tggaacatac ccagaccacc gacctttttt atgctgttac ccatacctgg
     9301 cctcctgata atacgcggtc gatgtgtgcg gcaaaatggg ccagattaaa tggttttgcg
     9361 ataaacccgt ttgcgcctgc tgcccttgcc tttggaagtg caatttccca atcggtggca
     9421 g
//
LOCUS       DAOQLC010000146         9343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2877_length_9343_cov_21.831503, whole genome shotgun sequence.
ACCESSION   DAOQLC010000146 DAOQLC010000000
VERSION     DAOQLC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2877_length_9343_cov_21.831503"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PL000_18355"
     CDS             complement(<1..1399)
                     /locus_tag="PL000_18355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC90900.1"
                     /translation="MTQILFGQSYYLRFDPKLWDAMMPYPPLGTLYAASAIRARGYDV
                     ALFDAMLADSEEEWARSLDEHRPEYAVIFEDNFNYLSKMCLLRMREAAFTMIRMAKER
                     GCTVILCGADATDHYSQYLERGADYCLLGEGEETLAELLDQLSAGKDAEGLPGLVSKR
                     STHALRRPDIKDLDALPFPAWDLVDVQKYKKIWLERHGYFSMNMVTTRGCPYHCNWCA
                     KPIWGQRYNSRSPENVAAEMKWLKENFAPDHIWFADDIFGLKPNWVERFAELLHELDA
                     VIPFKCLKRADLVTEKTASALAKAGCKTVWLGAESGSQKILDAMDKGDQVEDIHRAAH
                     LLHENKIEVGFFLQFGYPGETWADVQKTLKMVRECMPDDIGISVSYPLPGTKFFERVK
                     MELGEKQNWVDSDDLAMLYHGPFPQEFYRILHGRVHHEFRLRRAWKKKDLRGMIRTPY
                     YLLGVAKSEYMLRKFL"
     gene            complement(1396..2256)
                     /locus_tag="PL000_18360"
     CDS             complement(1396..2256)
                     /locus_tag="PL000_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90901.1"
                     /translation="MDLKQAVLETLAYSDVFDHPLTLGELRKFLVAPASQAELQACAA
                     GMESVAYANGFYHLAHRTDILEIRKLREKNSKSAFKRAEFYGRILGRLPFIRMVAMTG
                     SLAMLNLSKNKDMDYLIVAKAGRVWTARAFALLFGRLARMVGDVICPNVIVSENALAW
                     EAKNLYTAREFAQMIPLSGWGVYRRLLNVNAWVADFLPNAFSDLDESPRENSSLTQRL
                     GESFLNRGLGDAFEKWEMTRKIARLQAQEGYGTETNFSAEICQGNFDHHGTWAMKAFQ
                     DRVSSLQVEK"
     gene            2360..3547
                     /locus_tag="PL000_18365"
     CDS             2360..3547
                     /locus_tag="PL000_18365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90902.1"
                     /translation="MHLTVVSPFPPTITGIGQYGFHITRAIEQAGLFSRITVLAGAKT
                     ESHNSRLLNNAEIDYCWRPDDLRTRGVILSRLKHLQPDLVWFNLGASVFGRSPLANLS
                     AMFSPMLAQRMGLPTIVTLHELVELADLRALNAPGGFLAPLGARLLTEVATKADLVCL
                     TMQHYVDWLSARNINCVHIPIGSYHEPELLEETDEQELLFFTTLAPFKGLEILLKAFK
                     ELKNDFPKLRLSIAGADHTRFPNYSKDLRKQCDEANSIRWLGQIPEEKVKDLFRSAQI
                     VVLPYTASTGSSSVLYQSATWGRAIVASDLNEHKHLARENGLQIQFFENGSVQSLKDS
                     LCNLLKQNSLRKSQTLHNFNAIQKLTPSETCRHYIQAFNSALESCRSQKRIIIPRKEM
                     ELL"
     gene            3544..6474
                     /locus_tag="PL000_18370"
     CDS             3544..6474
                     /locus_tag="PL000_18370"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC90903.1"
                     /translation="MIPRRVLLAVLFASLLHGFFVLTARYRLSYDAYNHMLFADHYAA
                     DWFSLWETRWYTGFSVVSYPPLTHQLIALFIPILGFDAAYALVLWVVTSLYPLGVYAF
                     SRIFTSRGASSYAALASALLLPIYVTAHIFGQLPFLTATLFTFFGMAALAKYAREGGL
                     RNLLLAVSFTATTMAAHHATLMIQPFFIFAIAVFQLGKNTWKHLVIRLGTFAALAIPA
                     GLAVIWPFWVWGTQQELQTPIDHLSRHNFLAEPFARTIFFWPFYFPLIAVVPFIFYKW
                     GKKFIGLQFSFVILFILGLGGTTKLPALLFGKSWEWLTYDRFAFWASLSLLPFFGILF
                     IKFHRRMKTRFALNLKPATLRKNLIPTLTFLIIASGAIGSWFTPKFFPIQPDPVDMQP
                     IVDFMAAEDHSYWRYITFGFGDQFAYLNLLTKNATTLDGSYHTARSIPELRESGIGQI
                     DTAFWTQQGIPAVAPILQASGKYGVRWGFVNLRLFVPELKKNGWEFVKYLPNGIQVWE
                     NPDFTFQPPIIPPEDPFESFSWGILPMLSLVTTLALGTVTFWQERGIRLVQNAYAVVV
                     SIIPLSLGLWYYKTIFEFKHTQVYFTYDHALFFLSDALALTAILLWLAATPQVRLNLF
                     HKLFFALCSWITLSAAWSLDWRTSLYIAGHFWLISLLILSLQDQPKAWTGIFAGAILA
                     LTLQGIIGWSEFRSQSTQVLERLNLPWPGLISASSKGAGILKYANGENFLRVYGSFPH
                     PNILGGFAVFGIAAATALLLRKRESLLIWAAPIVGAVVIALSFSRSAWLGASAFFIVL
                     LLKAKFLEKKKLWAILALLALAFAVVMFPLRELFLSRTTAATSNTEKFSLVGRAWLSQ
                     QAWSYIKEKPLTGIGAGSFVIQLAQRSGEFNFVEPVHNVPLLVLAELGPIGLGLLLAL
                     AFVMTRQIWRSHQSGVIVTSALLAGLGVITLFDHYLWSLAPGRMLFGLALGLWQGQVQ
                     AAHE"
     gene            6467..7474
                     /locus_tag="PL000_18375"
     CDS             6467..7474
                     /locus_tag="PL000_18375"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNC90904.1"
                     /translation="MNKIAVNGRCLTRRITGVERYAREISKRLAGSHILQPQNGTGQV
                     AGNLWEQVILPGQLKHDEILWSPANAGPWLVKNQVITIHDASVFDHPEWFKPAFAAWT
                     RLSWQMLARRVRAIITVSNFSCERLKHHLKIPAEKIHVVHNGVGKPFAPQAQPHIDAV
                     RSKYGIQKPYFLFVGTIEPRKNLGGLLQAWNRAGITTHELFIAGARGRVFQPISGAPN
                     IVYNVHDDDLPALYSGAAAFIIPSFYEGFGLTALEAMACGTPVIASRTTVFPETLGEA
                     AQFIDPYSVNQLSDAMRAILEDSALAATLRKRGLEHASRFSWDESARKTQEIICAAGG
                     S"
     gene            7471..8565
                     /locus_tag="PL000_18380"
     CDS             7471..8565
                     /locus_tag="PL000_18380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC90905.1"
                     /translation="MKLALVHEWLNVYGGSERLLTEILQLYPQADLHALIHNPVNLQN
                     TPLAGRRVKTSFLQNIPHVEKLYRGLLPLMPLAIENMNVRNYDVVLSISHAVAHGIKT
                     HKGQTHISYVCTPMRYAWHLQDDYLHLHHLDKPLVGAAARLTLSLLRRWDRESASRAD
                     HLLAISQWTAERIRQAWGRESQVIHPPVNVNRFSPADEREEFYLLVSRLVPYKMTTEI
                     IMAFNELKLPLLIAGDGPEMPRLQKLAGSNVRLLGHQPDSVVTDLMNRAKAFLHMAVE
                     DFGIAMVEAQAAGCPVIAYEKGGAAEIVRNGETGLLFAQQNAKALVEAVVKFKSLKLN
                     SKAASMNAARFSTERFTKEFLSYMENLCGN"
     gene            complement(8574..9164)
                     /locus_tag="PL000_18385"
     CDS             complement(8574..9164)
                     /locus_tag="PL000_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90906.1"
                     /translation="MNTIKWIQKYSTGRNVAIFFSLNMVFYLAILLYSIPLVGRSAPS
                     MKLFDVSPSGYTKEYAVTLLTAIGSEGRDLYLSLQLPLDFVYPGLFIVSYSLMFAWLL
                     RKNYDLRSKIHYVLYLPIFAGLFDYLENVFVILMLKTFPDLSAGLVSAASFATVAKSI
                     LSSVFFTLLLLSIVQTLWRAAFKRADTAPVPDSRRV"
BASE COUNT         2209 a         2765 c         2161 g         2208 t
ORIGIN      
        1 ataaaaactt tctcaacatg tattcgctct tcgccacgcc caacagataa tacggcgtgc
       61 ggatcatgcc gcgcaggtct tttttcttcc atgcacggcg cagacggaat tcatggtgaa
      121 cccgtccatg caggatgcgg taaaactcct gtggaaacgg tccgtggtaa agcatggcga
      181 ggtcatctga atccacccag ttttgcttct cgcccaattc catttttaca cgctcaaaaa
      241 atttcgttcc aggcagcggg taggaaaccg aaatgccgat gtcatcgggc atgcactcac
      301 gtaccatttt caaggtcttt tgcacatcgg cccaggtctc accggggtag ccaaattgca
      361 aaaagaaacc gacctcgatc ttgttttcat gcagcaaatg cgcggcgcgg tgaatatcct
      421 ccacctgatc gcctttgtcc atcgcatcca aaattttctg cgaccccgac tccgcgccaa
      481 gccacactgt tttacagcct gccttcgcaa gcgccgaagc cgttttctcg gtcacaaggt
      541 cggcgcgctt caaacacttg aacgggatga ccgcatccaa ctcatgcagc aattccgcaa
      601 accgctccac ccagtttggt ttcaagccaa aaatatcatc ggcaaaccaa atgtgatcgg
      661 gagcaaaatt ctccttcaac cacttcatct ccgccgccac gttctcaggc gagcgcgagt
      721 tataacgctg gccccagatc ggcttggcgc accaattgca atggtacggg cagccacggg
      781 tcgtcaccat gttcatcgaa aaatatccgt gccgctcaag ccagatcttt ttatacttct
      841 gcacatccac cagatcccag gcagggaatg gcagcgcatc caggtctttg atatcgggac
      901 ggcgcaaggc atgtgttgac cttttggata caaggccagg aagcccttcc gcatccttcc
      961 ccgccgacaa ctggtccagc aattctgcaa gagtctcctc cccttcgccc agcagacagt
     1021 aatccgcgcc gcgttcgagg tattgactgt aatggtcggt cgcatcagcg ccgcacaata
     1081 tgaccgtgca tccgcgctct ttggccatgc ggatcattgt gaaggccgct tcgcgcatcc
     1141 gcagcaggca catcttggaa agataattga aattatcttc gaaaatgacc gcgtattctg
     1201 gccgatgctc atcaagcgac cttgcccatt cttcttccga atcagccagc atcgcatcga
     1261 agagcgccac gtcatatcca cgcgcgcgga tggcgctggc cgcgtacaaa gtccccagcg
     1321 gcggatatgg catcatggca tcccaaagct tggggtcaaa ccgcagatag taggattgtc
     1381 caaagaggat ctgtgtcatt tttcaacctg taaacttgaa accctgtctt gaaaagcttt
     1441 cattgcccac gtgccgtgat ggtcaaaatt accctggcaa atctccgctg aaaaattcgt
     1501 ctctgtgcca tagccttcct gcgcttgcag gcgggcgatc ttgcgggtca tctcccactt
     1561 ttcgaaggcg tcacccagcc cacggttaag aaaagattcg ccaagccgct gagtcaggga
     1621 actgttctcg cgtgggcttt catccagatc agaaaaagca ttcggcagaa aatccgccac
     1681 ccaggcattg acgtttaaca aacggcgata cacaccccag ccgctgagcg gaatcatttg
     1741 cgcgaattcg cgggcggtgt aaagattttt tgcctcccac gccagcgcat tttccgaaac
     1801 gatcacattc gggcaaatga catcgccaac catgcgcgcc agcctgccga acagcagggc
     1861 aaaggcccgt gccgtccaca cccgccctgc tttggcaacg atcaaataat ccatgtcctt
     1921 gtttttcgaa agattgagca tcgccaggga acctgtcatt gcgaccatac gaataaaagg
     1981 cagccgaccc aaaatgcgcc cataaaattc ggctcgcttg aaggcgcttt tggaattttt
     2041 ctcacgcagt tttcgaattt ccaaaatatc ggtgcgatga gcaaggtgat aaaaaccatt
     2101 tgcataagcc acagattcca tgcccgccgc acaagcctgc aactctgcct gtgaagcagg
     2161 cgcaactaaa aacttacgca gctcacccag ggtcaatgga tgatcgaaca cgtcggaata
     2221 tgcgagggtt tcaagaacag cctgcttcaa atccataata ggagacgaac tatactttgc
     2281 aagttaaaat cctgcaacgt atagttgccg tgctacgcgt tgtctaattc tcacattaat
     2341 ggaataattc cagcttctta tgcatttaac cgtagtcagc ccattccctc caacaattac
     2401 cggtattgga caatacgggt ttcacatcac aagggccatt gaacaggcag gtttgttctc
     2461 tcggatcacc gtccttgctg gagcgaaaac cgagagtcac aattcccgtc ttctgaacaa
     2521 cgcagagata gattattgct ggcggccaga tgacctgaga acgcgcgggg tcattctgtc
     2581 gcgcttgaaa cacttacagc ctgatctggt ctggttcaat ctgggtgcga gcgtctttgg
     2641 caggtcaccg ctggcaaacc tttcagccat gttctcgccc atgctcgcac agagaatggg
     2701 actgcccacc attgtcaccc tgcatgaact cgtggagctt gccgacctgc gcgcactcaa
     2761 tgccccaggc ggattcctcg ccccgctcgg cgcgcggctg ttgaccgaag tcgccaccaa
     2821 agccgacctt gtctgcctga ccatgcagca ctatgtagat tggctctccg cccgcaacat
     2881 caactgtgtg catatcccca tcggctcgta tcacgagcct gaacttttgg aagaaaccga
     2941 cgaacaggaa ttgctgttct tcaccacatt ggctcccttc aaagggctgg agattctgct
     3001 caaagccttc aaagaactga aaaacgattt tccgaagtta cgcctttcca ttgctggcgc
     3061 agaccacacc cgctttccca attattccaa agacctcaga aagcaatgcg acgaagcgaa
     3121 cagcatccgc tggcttgggc agattcccga agaaaaagta aaagacctct tccgcagcgc
     3181 acagatcgtg gttctgcctt acaccgcttc cacggggtct tccagcgttt tgtatcaatc
     3241 ggccacctgg gggcgcgcca tcgttgcctc tgacctgaac gagcacaaac acctcgccag
     3301 agaaaacggt ctgcagatcc aattctttga aaacggcagc gttcaaagcc tgaaagattc
     3361 gctctgcaac ctgctaaagc agaactctct gcgcaaaagc cagaccctgc acaacttcaa
     3421 cgccatccaa aaactgactc ccagcgaaac ctgccgtcat tacattcagg ctttcaattc
     3481 cgcactggaa agctgccgca gccaaaaacg gatcatcata ccgcgcaagg aaatggaact
     3541 gctatgatcc cgcgccgcgt gcttctggct gttctcttcg catctctgct gcacggattt
     3601 ttcgttctca ccgcgcgcta ccgcctgagt tacgatgcct acaaccacat gctctttgcc
     3661 gaccactacg cggcagattg gttctcgtta tgggaaacac gctggtacac gggcttcagt
     3721 gtggtttcct acccgccgct cacacatcaa ctcattgcac tcttcatccc catcctcggc
     3781 ttcgatgccg catacgccct tgtgctttgg gtggtcacct ccttgtatcc gctgggtgtg
     3841 tatgccttca gcagaatctt cacaagccgc ggcgcttcat cgtacgctgc tctggcatcg
     3901 gcgcttttgc tgcccatcta cgtcactgcg cacattttcg gccagcttcc atttctcacg
     3961 gccaccctgt tcaccttctt tggcatggcg gccctcgcaa aatacgcacg ggaaggcggc
     4021 ctgcgcaatc ttttactggc ggtctcattc accgccacca ccatggctgc tcatcatgcc
     4081 actctgatga tccagccgtt ttttattttc gccattgccg tctttcaact tggcaaaaac
     4141 acatggaagc atctcgtcat caggctgggc acatttgccg cgctggcgat ccctgcgggg
     4201 ttggcggtca tctggccgtt ttgggtctgg ggcacacagc aggaactaca aacccccatt
     4261 gaccatctct cccgtcacaa ctttctcgcc gagccatttg cgcgcaccat ttttttctgg
     4321 ccgttctatt ttccgctcat cgctgtggtc ccattcattt tttacaaatg gggcaaaaaa
     4381 ttcatcggtt tgcaattttc attcgtcatc cttttcatcc tcggccttgg cggcaccacc
     4441 aaactccccg ccctgctctt cggcaaaagc tgggaatggc tcacgtacga ccgcttcgca
     4501 ttttgggcat cgctcagcct gctcccgttc tttggaatcc tcttcatcaa attccacagg
     4561 cggatgaaaa cccgcttcgc ccttaacctg aaacctgcca cgcttcgaaa gaacctgatc
     4621 ccaaccctga catttctcat cattgccagc ggcgccattg gttcatggtt cacgcccaaa
     4681 ttttttccca tccaacccga ccccgtggac atgcaaccca tcgttgattt catggctgcc
     4741 gaagaccatt cctactggcg ttacatcacc ttcggtttcg gcgatcaatt tgcctacctc
     4801 aacctgctca ccaaaaacgc caccaccctc gatggaagtt atcacaccgc ccgctccatc
     4861 cccgaattgc gcgaaagcgg aatcggccag atcgacaccg ccttctggac ccagcaggga
     4921 atccctgccg ttgccccgat cttgcaagcc tcgggaaagt acggcgtgcg ctggggcttt
     4981 gtcaacttgc ggctgtttgt ccctgagttg aaaaaaaacg gctgggaatt tgtcaaatac
     5041 ctgccaaacg gaattcaagt ttgggaaaat ccagatttca catttcagcc gccgatcatt
     5101 ccgcccgaag acccttttga atctttttca tggggcatcc tgccgatgct ttcgctggtc
     5161 accacgcttg cgcttggcac ggtcactttt tggcaggaac gcggcattcg actcgtccaa
     5221 aatgcgtatg ccgttgtcgt gagcattatt ccgctcagcc ttggcctctg gtattacaaa
     5281 accatttttg aattcaaaca cacacaggtg tatttcacct acgaccatgc cctctttttc
     5341 ctcagcgatg cactggcgct gacagccatc ctgctctggc ttgccgccac tccacaagtc
     5401 aggctcaact tattccataa attattcttt gccctttgct cctggataac gctcagcgcc
     5461 gcatggtctt tggattggcg cacttctttg tacattgctg ggcatttttg gctgatctcc
     5521 ctgctcatcc tctctttgca ggatcaaccc aaagcgtgga cagggatctt tgcaggcgca
     5581 attcttgcgc tgacgcttca aggcatcatc ggttggagtg agtttcgatc tcaatctaca
     5641 caagtgcttg aacggctaaa cctgccgtgg ccaggtttga tcagcgcttc ttccaaaggt
     5701 gctggcattt tgaaatacgc caacggcgaa aactttttgc gcgtttacgg ttcattccca
     5761 cacccgaaca ttttgggggg ctttgctgta tttggaattg cagccgccac agccttgttg
     5821 cttcgcaaaa gggagagtct gcttatttgg gccgcaccaa ttgtcggcgc ggtggtgatc
     5881 gcgctttcct tttcacgctc cgcctggttg ggggcatccg cttttttcat tgtgctgctg
     5941 ctgaaggcaa agtttcttga aaagaaaaaa ctctgggcca tccttgcact tctcgcgctt
     6001 gcatttgctg tggtcatgtt tcccttgcgc gaactttttc ttagcagaac aaccgccgcc
     6061 acaagcaaca cagaaaaatt ttcactcgtc gggcgcgcct ggctcagcca acaggcatgg
     6121 agctacatca aggaaaagcc gctgacaggc atcggcgcag gttcatttgt aatccaactt
     6181 gcccaacgtt caggcgaatt caattttgtt gagcctgttc acaacgtccc cctgctggtt
     6241 cttgcagaat tgggaccaat cgggctcggc cttctgctgg cattggcctt tgtaatgacc
     6301 cgccaaatct ggaggtcgca tcagtcgggc gtgatcgtca caagcgcact tttggcgggc
     6361 cttggtgtca tcaccctctt cgaccactat ctctggtcgc ttgcgccagg ccgcatgttg
     6421 tttggactcg cactcgggct ttggcaggga caagtgcagg ccgcccatga ataaaattgc
     6481 cgtcaatggc cgctgcctca cccgccgcat tacaggcgtg gaacgctacg cccgcgagat
     6541 cagcaagaga cttgcaggca gccacatcct tcagccgcaa aacggtacgg ggcaggttgc
     6601 agggaatctt tgggagcagg tcattttgcc tggccagcta aagcacgacg aaattctttg
     6661 gtctccagcc aatgcaggtc catggcttgt gaaaaatcaa gtcatcacca tccatgatgc
     6721 cagtgtcttc gatcatcccg aatggttcaa acccgccttt gccgcctgga cgcgactctc
     6781 ctggcagatg cttgcccgca gggtcagggc catcatcacg gtttccaatt tttcatgtga
     6841 acggctgaaa catcacctca aaatccccgc tgaaaaaatt catgtggtcc ataacggtgt
     6901 tggaaaaccc tttgcgccgc aagcacaacc acacatcgac gcagtgagat cgaaatacgg
     6961 aatccaaaaa ccatacttcc tgttcgttgg caccattgaa ccgcgcaaaa acctcggcgg
     7021 cttgctccag gcatggaatc gggctggcat cacaacccac gaacttttca tcgctggcgc
     7081 cagaggcagg gtctttcaac ccatcagcgg cgcacccaac atcgtttaca acgtgcatga
     7141 tgatgacctg cccgccctgt attcgggtgc cgccgcattt atcatccctt ctttttatga
     7201 aggcttcggc ctgaccgccc tcgaagccat ggcttgcggc acacccgtca ttgcctcgcg
     7261 caccaccgtt ttccctgaaa cactgggaga agcggcgcaa tttatagatc catattctgt
     7321 aaaccaacta tccgatgcca tgcgggccat cctcgaagac tctgcacttg cggccaccct
     7381 gcgcaaacgc ggcctcgaac acgcatcccg tttttcatgg gacgaatcgg cgcgaaagac
     7441 tcaggaaatc atctgcgcgg caggcggttc atgaagctcg ccctcgtcca cgaatggctc
     7501 aatgtgtatg gtggttcgga acgtctgctg accgagatct tacagttgta cccgcaggcg
     7561 gatcttcacg cattgatcca caaccccgtc aatctgcaaa acacaccgct tgcaggccgc
     7621 cgcgtcaaaa catcctttct gcaaaacatc ccccatgtgg aaaaactgta tcgcggcctc
     7681 cttccgctga tgccgctcgc catcgagaac atgaacgtgc gcaattatga cgttgtcctg
     7741 tccatttcac atgcggttgc acacggcatc aaaacgcaca agggtcagac tcacatttcg
     7801 tatgtttgca cgccgatgcg ctacgcctgg catttacagg atgactatct ccacttgcat
     7861 catttggata agcccctggt cggggccgcc gcccgcctga ccctgagcct gctccgccgt
     7921 tgggacagag aatcagcatc ccgagccgat catctgctcg ccatctcaca atggacggcc
     7981 gaacgcatcc gtcaggcctg ggggcgcgaa tcgcaggtca tccacccgcc cgtaaacgtc
     8041 aaccgtttct cgcccgccga tgaaagagag gaattctatc ttctcgtttc gcgcctcgtg
     8101 ccatacaaaa tgaccaccga gatcatcatg gctttcaacg agttgaaact tccgctgctc
     8161 atcgctggcg atggacctga aatgccgcgc ttgcaaaaac tggcaggaag caatgtccga
     8221 ttgctaggcc atcaacccga ttcagtcgtc acagacctga tgaatcgtgc aaaagccttc
     8281 ctccacatgg ctgtggaaga ttttgggatc gccatggtcg aagcacaggc agcggggtgt
     8341 cccgtcattg catacgaaaa aggcggcgcg gctgaaattg tccgcaacgg ggagactggg
     8401 ctgttattcg cacagcaaaa tgcaaaagcg cttgtggagg cagtcgtaaa gttcaaaagc
     8461 ctgaaattaa acagcaaagc tgcatccatg aatgcagctc gcttttcaac tgagagattt
     8521 acaaaggaat tcctctcgta catggaaaat ctctgcggaa attagtcaac gcctcatacg
     8581 cgccgcgaat cgggcacggg cgcggtatcc gctcttttga atgcagcccg ccaaagggtc
     8641 tgcacgatgc tcaaaagcag caacgtaaag aaaaccgaag acaggatact ttttgcaacg
     8701 gtcgcaaaac tggcggcaga tacaagacca gcagaaaggt cagggaaagt cttcaacatc
     8761 agaatgacaa aaacattttc cagatagtcg aacaaccctg caaagatcgg aagataaagc
     8821 acgtaatgga tcttcgaacg caagtcataa ttctttctga gcagccacgc aaacatcagg
     8881 gaatatgaga cgatgaacaa acccgggtaa acaaaatcca gcggcaactg caacgataag
     8941 tacaagtccc tgccctcaga accaatggca gttagcagag tcaccgcata ctcctttgtg
     9001 taccctgagg gagaaacatc gaacaatttc atgctggggg cagatctgcc caccaacgga
     9061 atcgagtaaa gcaagatcgc aaggtaaaaa accatattca gagaaaagaa gatggcgaca
     9121 ttccttcctg ttgaatattt ctggatccat ttgatggtgt tcataaaaaa cctcttcgat
     9181 aaaaaactca acaattggca acttacttct tatgtgggtg actcttgtac caagccacgg
     9241 ttgccttgat ggcatccttc atcggtgttg cctgcatacc gaaggttttc tcgaacttgc
     9301 ttgaatcaac gatgaacggt ttttcgaact cgtacatcat ctc
//
LOCUS       DAOQLC010000147         9267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2901_length_9267_cov_24.741641, whole genome shotgun sequence.
ACCESSION   DAOQLC010000147 DAOQLC010000000
VERSION     DAOQLC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_9267_cov_24.741641"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            423..1301
                     /gene="panB"
                     /locus_tag="PL000_18390"
     CDS             423..1301
                     /gene="panB"
                     /locus_tag="PL000_18390"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC90907.1"
                     /translation="MSTVTPVSTSTSRKKVTTQTFRQKKERGEPITMLTAYDYPTAMA
                     MDKAGVDSILVGDSLAMVVLGYENTLPVTMEEMLHHARAVARGARSALLVGDMPFMSY
                     QVSAEEALRNAGRFLQQGGMDAVKLEGGRERADAVRAIVGAGIPVMGHIGLTPQSVHQ
                     LGGFRAQGKTASAAKRLLEDAKILEDSGAFSLVLESVPARLAEVISKQISIPTIGIGA
                     GVGCDGQVLVTHDLLGLFDRFTPKFVKQYANFHAEMNKAFTAYIDDVETKRFPAVEHT
                     VEMTDEEWNAFQEEIK"
     gene            1298..2212
                     /locus_tag="PL000_18395"
     CDS             1298..2212
                     /locus_tag="PL000_18395"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNC90908.1"
                     /translation="MKQDILLVGTGALATLFAARLSEAGHGVHMLGTWQKGLAELKQH
                     GARVVDANGNERAFQVHATDDPREVSGAKFAIVLVKSWQTVRVAKQLKDALAADGHAL
                     TLQNGLGNFETLALDLGMGRVSMGVTTTGATLVGPGLVRAGGEGVISLEQNQALGPLE
                     AALRSSNFNLQTVHDARSLIWGKLVINAAINPLTALLQVPNGELLAHPKARRMMGELA
                     RETAEVAEAEHVHLPFSNPVSAAEDVARKTATNHSSMFQDVRRGAPTEIDAICGAVMK
                     MGQKHGIRTPYNKACWTMVRASVSHALN"
     assembly_gap    2307..2406
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2440..3342
                     /locus_tag="PL000_18400"
     CDS             2440..3342
                     /locus_tag="PL000_18400"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNC90909.1"
                     /translation="MRKFLLLIMLGLLFAFPASVFAQSDLKLTNVNVQLWPEYDQPSM
                     LVITDFEIPATTALPVNVTFRIPQDANLIAVAVYSADGALTNAIFEGPQEDGEWQSFT
                     VSLDSTAGRFEYYQTLSFNGNQRIFSYLWDGIYAVDTFAIRVLEPFDTTSLKTDPALT
                     SISQESNLKYYEGEPVTLASGEQFILNLEYEKTTDTLIVNTQGVQPSAPVDENTPGRV
                     SLSNSLPYIIGGLGVIMIVGGVVYYWQSGRRSTGGKSRKRSRSHAEEQEDGEETYCPQ
                     CGSRSKTGDRFCRTCGARLRHQEE"
     gene            3398..4885
                     /locus_tag="PL000_18405"
     CDS             3398..4885
                     /locus_tag="PL000_18405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC90910.1"
                     /translation="MNQDPLIVIYFVYGLAFFSMGLLVAMEGGRSTDARLRMALRPLA
                     GFGLVHAIHEWLEMFKVMGHFNEIAAAVYPVVGLSVLAFSFLSLAAFGAYLVLGSEST
                     WRVSLIIPLALEAIWVYGLLTFKGIYSIDDMYVIADVWTRYSIAIPAGLLAAAGLVVQ
                     QRAFRRAGLVSFGRDSLWASVAFGWYSLVGQIFTAPSHLAPSTFLNSTLFLHLFGFPV
                     QLLRAVTAIFASVFVIRFLRAFQVESDHKIAELQEARLHESQNREQLRGELFRKVVGA
                     QEAERQRIARDLHDETGQSLTAIGMGLRGLSDETNEKKRTATLGQLQSLTSDALKELQ
                     RIIADLRPAHLDDLGLSATLRWYASRVHELTSIHVRVDIAGDEPGLDDAVKITIFRIV
                     QEALNNIIKHADATNVNVHLDYHENKIKIFIRDNGRGFDMDAVQSRIGRTSLGLAGME
                     ERAMLLGGVVDVRSRPNYGTEIEAVIPYQLKKVEGSKNDTPVAGG"
     gene            4860..5507
                     /locus_tag="PL000_18410"
     CDS             4860..5507
                     /locus_tag="PL000_18410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90911.1"
                     /translation="MTRLLLVDDHAVVRSGLKMLLSGHEEMEIVGEAGSAAEAMREAE
                     RTTPEVILMDIGLPDKTGIEATRDIKKRFPEMKIVALTIHEDEEYFFQMLDAGASGYV
                     PKRAAPDELITAIRAAAAGEVYLYPSMAKLLVHDYLNAERPAEEKLNLDGLTDREREV
                     LTHLAEGAGNDEIAEALVISPKTVERHRENIMRKLNLHSRSELVRYAIRKGIIKA"
     gene            5922..6263
                     /locus_tag="PL000_18415"
     CDS             5922..6263
                     /locus_tag="PL000_18415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90912.1"
                     /translation="MNTTLAFVIGVLLRLIVPLTVTALVVFVLHRLDVRWQAEAEKER
                     AALVKDNMPCWKEEGLSVDEIMVRAAKNEQPCWQTHRLSNGYLREACLDCEVFLAAPA
                     PAAKHINAHVS"
     gene            6276..7271
                     /locus_tag="PL000_18420"
     CDS             6276..7271
                     /locus_tag="PL000_18420"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNC90913.1"
                     /translation="MFSRFEKALIALMFALVFAGVTLVAVSAQDGTPPAAQEENDDCA
                     ECHEDSQMSWQNGPHGNAKSDPIFVSAWTEQGQPGACLTCHVTGYDEATGTWKEDGVT
                     CAACHKDEGGDHPKTSMTVDPTSNTCGNCHTDTRFIAEESWQGSAHYQNGMDCTSCHD
                     PHNASLKITINLKDPNAANDASRLCISCHEEASMNFPYSSHSQQDITCIDCHLEHLEN
                     PNKDIHAVPDHSFKASIKTCSTCHAEQMHAAGNVVSTKEAASVSVTETEPTAEPTPAV
                     EMSSIVPEPTPVSPIGYAGLAALIGLAAGMVLAPWLERWYRLILKKSEEVRHDGK"
     gene            7258..8037
                     /locus_tag="PL000_18425"
     CDS             7258..8037
                     /locus_tag="PL000_18425"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC90914.1"
                     /translation="MTENNLNRRDFIKISAVGLAVAAGMRVAQGEEVGASSATKGEHQ
                     WVMVIDQSKCTGCEQCNLACHAHNDINPELQWTKVLDAGEVNGEKVYLPRPCMQCEHA
                     PCVEVCPVGASYYRDDGIVMMDYDKCIGCRYCMVACPYDARAFNWKEFTGPNPAVPEW
                     GEPEVDRRPRGVPEKCSFCYNRIDRGMSVGLVPGVDAEATPACVVTCPTGARIFGDLN
                     DPHSNVSQVLKKHPSYRLRENLGTNPRVYYLPVREDGVVED"
     gene            8039..>9267
                     /gene="nrfD"
                     /locus_tag="PL000_18430"
     CDS             8039..>9267
                     /gene="nrfD"
                     /locus_tag="PL000_18430"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNC90915.1"
                     /translation="MKTILKTIRENIFTPWGLWLGFLGMFLVAALVAGLSVFWFGLSL
                     TNLTDIVPWGLWITIDLSSIAVAAGAFSLCAIVYLFGFKRYEPIARTATFIGFTGYSM
                     AMMALLLDIGRPDRFWKSFVYWNTHSLLWEVTMCVGLYFTVLVLEVLPIVASFDWLRK
                     KFPRIAEKMEHLHHYAPVLAIFGLGLSSLHQSSLGAVYGVIKARPIWYRPEMSVMFMF
                     TAILGGVALTLFASMLSARLTSKAKVNDILIERVSQVLGYMMIAYLYWRFWDWLAQTY
                     TYEPGRTEAFELLTKGTLSFNFWFGEMLFGALIPTVILLYKPTRLSPFWRMLGLAMIV
                     GGVVAFRFDTNIVGFLAVIPYLPGEAVISYTSYSPSLVEWAAGLGIIAFGLLAFSIGV
                     KYLRVVDHRLTSEEYETV"
BASE COUNT         2035 a         2330 c         2545 g         2257 t
ORIGIN      
        1 agcagcaatg gggtaaataa aaacgccccg cgttttccgc gaggcgttgt gtgtttcgag
       61 agtaacttcg ttcggttact tcatcccaag cactaacgcc tcgcggtggg taccggaggt
      121 agtagtggtc atggtcatgg agaagtgagc cgagttcaaa gtgctgtgca tgttgggcgg
      181 tattctatgt gaatgaacgg attgagtcaa gggtgaaatt gtgtttgtaa gaaagtccct
      241 gcagcaatgg tcaatacaat gtgacacaat agccaaaaac ttttttttgt gtcataatac
      301 gtcagccgca tgtatccaaa agaacgtgac ggcgaaaatc aaatcaagcc gaaatcgtgt
      361 gcatatcagg ggcgaaagca cacaacgcgg actctacctt tgccaaaggc acggagggta
      421 ccatgtcaac tgttacacca gtctcaacca gcacgtcacg taaaaaggtg actacgcaga
      481 cgttccgtca gaaaaaggag cgcggcgaac cgatcaccat gcttaccgcc tatgactatc
      541 ccactgcaat ggcgatggat aaggcgggtg ttgattccat tctcgtgggc gattcgcttg
      601 cgatggtggt cttgggttat gaaaacacat tacccgttac gatggaggaa atgcttcatc
      661 acgcacgggc tgtggcacgc ggcgcaagat cggccctgct tgtcggcgat atgcctttca
      721 tgtcgtatca agtttcagca gaggaagcgc ttcgaaacgc gggccgcttt ttgcaacaag
      781 gcggcatgga tgccgtcaaa ctcgaaggcg ggcgcgaacg cgccgatgca gtccgcgcca
      841 ttgtgggggc agggattccg gtgatggggc atatcggcct cacaccacaa tcggtgcatc
      901 agcttggcgg gtttcgcgca cagggcaaaa ctgcctcggc agcaaaacgc ctgcttgaag
      961 atgcgaaaat tttggaagac tcaggcgcat tcagccttgt gctcgaatcg gtccctgcgc
     1021 ggctggctga ggtgatctcc aaacagatct caattccaac cattggcatc ggtgcagggg
     1081 tgggttgcga tggtcaggtg ctggtcacac acgatctgct tgggttattc gaccgtttca
     1141 cgccgaagtt cgtcaaacaa tatgcaaact ttcatgcaga gatgaacaag gcatttacgg
     1201 catatattga tgatgtcgaa acgaaacgat tccctgcggt tgaacatacg gttgaaatga
     1261 ccgatgaaga gtggaatgca tttcaggaag agatcaaatg aaacaggata ttcttctggt
     1321 tggtacaggc gcacttgcca ccctgttcgc ggcacgtttg agcgaagcgg gccacggtgt
     1381 tcatatgctg ggcacctggc aaaagggatt ggctgaactg aagcaacacg gcgcacgcgt
     1441 ggtggatgca aatgggaacg aacgcgcgtt tcaagtccat gccacggatg atccgcgcga
     1501 ggtgagcggt gcgaagtttg cgatcgtgct tgtcaaatca tggcagactg tacgcgtggc
     1561 gaaacagttg aaagatgcac tggctgcaga tggacatgcg cttacgcttc aaaatggcct
     1621 tggtaatttt gaaacgctcg cactggacct gggaatgggt cgggtttcaa tgggtgtgac
     1681 caccaccggg gcaacattgg tcgggccagg tttggtgagg gcaggcggtg agggagtcat
     1741 ctctcttgaa cagaatcagg ctctcggtcc gttggaagcg gctcttcgtt cgtccaattt
     1801 caatttgcag accgtccatg atgcgcgttc cctgatctgg ggcaagttgg tcattaatgc
     1861 ggccatcaac ccgttgactg cgctgttgca ggtgcccaat ggtgaattgc ttgcacatcc
     1921 caaggcccgt agaatgatgg gcgagttggc gcgtgagacc gctgaagttg ccgaggcgga
     1981 gcatgtgcat ttgccgttca gcaaccccgt cagcgcggcg gaggatgtgg cccgtaaaac
     2041 agcgaccaat cactcgtcca tgtttcaaga tgtgcggcgc ggtgcgccga ccgagatcga
     2101 tgcgatctgc ggcgcggtaa tgaaaatggg gcaaaagcac ggaatccgca ctccatacaa
     2161 caaagcctgt tggacaatgg tgcgggcttc ggtgagccat gccctgaatt aataagcgtt
     2221 ctgcacccct gataaaaagg gtgactttca tccataatag ttcgtgattt cgtttctgtc
     2281 cgctgtttct tgcgcatggt aagatannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnntaaa agaaactctt attgttggag gtttcttcca tgcgcaagtt tctgttgttg
     2461 ataatgttgg ggcttctttt tgcattcccc gcttccgttt ttgcccaatc cgatcttaag
     2521 ttgaccaatg tgaatgtgca gctctggccc gaatatgacc agcccagcat gctggtgatc
     2581 accgatttcg aaattcccgc cactacggcc ctgcccgtaa atgtgacctt ccgcattccc
     2641 caagatgcaa atctgatcgc tgtggctgtt tactctgcag atggtgctct aaccaacgcc
     2701 atatttgaag gcccgcaaga agatggcgaa tggcagtctt tcaccgtttc acttgattca
     2761 actgcagggc gtttcgaata ttaccaaacg ctttcgttca atggcaacca gcggatcttc
     2821 tcctatcttt gggatgggat ctacgcagtg gatactttcg ccattcgtgt gttggagcct
     2881 tttgatacga cctcgctcaa gaccgatcca gcgctcactt ccatttcaca ggaaagcaac
     2941 ctcaaatatt atgaagggga acccgtaacc cttgccagtg gcgaacaatt catcttgaac
     3001 ctcgaatatg aaaaaacgac cgacacactg atcgtcaata cgcagggtgt acaaccctcg
     3061 gcgcctgtgg atgaaaacac ccctggccgc gtttccttga gcaattcgct gccgtatatc
     3121 attggcggat tgggtgtgat catgatcgtg ggcggggtgg tgtattactg gcagtcgggt
     3181 cgccgctcca cgggcggcaa atcacgcaag cgttcccggt ctcatgctga agagcaggag
     3241 gacggcgaag aaacatattg cccgcaatgc ggctcccgtt caaaaacagg tgaccgtttc
     3301 tgccgaactt gcggcgcaag attacgacat caggaagaat gaccatgagg ccctgaaggt
     3361 ttttcaaaaa cctttggggt cttatacata aaaacacatg aaccaggatc cattgattgt
     3421 catctacttt gtgtatggcc ttgctttttt cagcatgggt ctgttggtgg caatggaagg
     3481 cgggcgttcc accgacgcac gattgcgcat ggcgttgcgt ccgctggcgg gatttgggct
     3541 ggtccatgcc atacacgaat ggctcgaaat gttcaaagtg atgggccatt tcaatgagat
     3601 cgctgcggcg gtttacccgg ttgttgggtt atcggtgctg gctttttcgt tcctgtcact
     3661 ggctgctttt ggcgcatacc ttgtactggg gagtgaatcc acctggcggg tcagcctcat
     3721 cattccactg gcattggagg ccatctgggt ttatggcctg ttgaccttca aaggaatcta
     3781 ttccatcgat gacatgtatg tcattgcaga tgtgtggaca cggtattcca tcgccatccc
     3841 agcagggttg ttggcggccg ccgggctggt cgtgcagcaa cgtgcatttc gacgtgctgg
     3901 gctggtaagt tttggacgcg atagtttgtg ggcctcggtg gcttttggct ggtacagcct
     3961 tgtggggcag atctttaccg caccgagcca tctggcgcca tccacttttt tgaattcaac
     4021 cttgttcctg cacctctttg gttttcctgt tcagctgctt cgggccgtca cagctatttt
     4081 cgcttctgta tttgtcatcc gttttttgcg tgcttttcaa gtggaaagcg atcacaagat
     4141 cgccgaactt caggaagcgc gtttgcacga atcgcaaaac cgtgaacaat tacgcggaga
     4201 gttattccgc aaggtggtgg gtgcccagga ggcggaacgt cagcgcattg cgcgtgacct
     4261 gcatgatgaa acgggtcaat cgcttacagc cattggcatg ggcctgcgcg gactttcaga
     4321 tgaaaccaat gaaaaaaagc ggactgcgac ccttggacag ttacagtcac tcacctctga
     4381 tgcgttgaag gaattacagc gcatcatcgc ggaccttcgg cctgcccatc tcgacgattt
     4441 gggcctgtct gccactttga gatggtacgc ttcgcgcgtc cacgagttga cttcgatcca
     4501 cgtgcgggtg gatatcgctg gcgacgagcc cgggcttgac gatgccgtga agatcaccat
     4561 cttccgcatt gtgcaggaag ccttgaacaa tatcatcaag catgctgatg ccacgaatgt
     4621 aaatgtccat ttggattatc acgaaaacaa gatcaaaata tttatccgcg ataatgggcg
     4681 cggcttcgat atggatgcgg ttcaaagccg catcgggcgt acctccctgg ggttggcggg
     4741 gatggaggaa cgcgccatgc tgttgggcgg tgttgtggat gtccgctcgc ggccgaacta
     4801 tggcacagag attgaagctg tgattcccta tcaattgaag aaggtggagg gtagtaaaaa
     4861 tgacacgcct gttgctggtg gatgatcatg ctgtggtgcg ctcggggctg aaaatgctgt
     4921 tgagcggcca cgaagagatg gaaattgtcg gtgaggcggg ttctgcggca gaggccatgc
     4981 gggaggccga gcggacaacc cccgaggtta ttcttatgga tattggtctg cccgataaaa
     5041 cgggcatcga agccacgcgt gatatcaaaa agagattccc cgaaatgaag atcgtggccc
     5101 tgaccattca cgaagatgag gagtatttct ttcaaatgct ggatgcgggt gcttcaggct
     5161 atgtgcctaa gcgggctgcc cctgatgagt tgattaccgc cattcgtgcg gcagcggcgg
     5221 gggaggtgta tctgtaccct tccatggcga aactgcttgt acatgattac ctgaatgccg
     5281 agcgccctgc cgaggaaaaa ctgaaccttg acggcctgac cgaccgtgaa cgtgaagtgc
     5341 ttactcattt ggctgaaggc gcagggaatg atgagatcgc cgaggccctt gtcatcagcc
     5401 cgaagacggt ggaacgtcac cgtgaaaaca tcatgcggaa gttgaatcta cactcccgct
     5461 ctgaattggt tcgttatgcg attcgcaagg ggatcatcaa ggcataaata gtgttagatt
     5521 ccgttttctt tggttgaatt aaaaccaatc ctgcttacgt aacctgaatt gtcgaattca
     5581 ccgacaggcc atccattcat gggtggcctg tcgatttaag gtgggggaga tcccccataa
     5641 aggtggggaa tgtgtggggt gtagtccgca ggtttgggcg gggaaagatg cgtgagtcct
     5701 gcgttcagga tgggggacga gccgaattta ctttctttta agggaagcgt atcctgtagg
     5761 caagatacag aaaagagaaa gaaaggagat tgccatgaac ggttcagaga tcattttttt
     5821 gctcttcgtc ttccgccttg tgatcccatt tggggtgctg atgctggtgg gcgaatggct
     5881 ccgtcgtaga gaatccaatt actggctgaa atgaggtccc catgaatacc acacttgctt
     5941 ttgttatcgg tgttttgctc cgcttgatcg tgccgctgac cgtgaccgcg ctggtcgttt
     6001 ttgtgcttca caggttggat gtgcgctggc aggcagaagc tgagaaggaa cgtgccgcgc
     6061 tggtgaaaga caatatgcca tgctggaagg aagagggtct ttctgtggat gagatcatgg
     6121 tgcgtgccgc taaaaatgaa caaccctgct ggcagaccca tcgtttgtcc aatggatatt
     6181 tgcgggaagc ctgccttgat tgcgaggttt tcctcgctgc acccgcacct gctgccaagc
     6241 atatcaatgc ccatgtgtca taaggagatg tgaaaatgtt ttcgcgtttt gagaaagccc
     6301 tgattgccct gatgttcgcc cttgtatttg cgggcgtgac ccttgttgct gtttcggcac
     6361 aggatggtac ccctcccgct gcccaggagg aaaatgatga ttgtgccgag tgtcatgaag
     6421 attcgcagat gagttggcaa aacggtccgc atgggaacgc caaatctgac ccgatctttg
     6481 tatccgcctg gaccgaacaa ggacagcccg gagcctgcct gacctgtcac gtgactggct
     6541 acgatgaagc cactggcact tggaaggaag atggcgtgac ctgcgcggcc tgccacaagg
     6601 atgaaggcgg cgaccacccg aagacctcca tgaccgtaga cccaacctcc aacacctgcg
     6661 gcaattgtca taccgatacc cgtttcatcg cggaagaatc ctggcagggc agtgcccatt
     6721 accagaacgg tatggattgc acctcctgcc atgatccgca taacgcttct ttgaagatca
     6781 cgatcaattt gaaagacccg aatgccgcca atgatgcttc gcgtttgtgc attagctgcc
     6841 acgaagaagc cagcatgaac ttcccgtact catcacacag tcagcaggat atcacctgta
     6901 ttgattgtca tttggaacat ttggaaaacc caaacaagga tatccacgcg gttcctgatc
     6961 actcgttcaa agccagcatc aagacctgca gcacttgtca cgcagaacag atgcatgcag
     7021 cgggtaacgt ggtttcgacc aaggaagcgg ccagtgtttc agtaacagaa acagaaccca
     7081 cagctgaacc cacccctgct gttgaaatgt cctccattgt gcctgagccg acccctgttt
     7141 ctccgatcgg gtatgcaggt cttgccgccc tcattggtct tgcggctggc atggtccttg
     7201 ctccgtggct tgagcgctgg tatcgcctga ttttgaagaa atctgaggag gttcgccatg
     7261 acggaaaata atttgaaccg ccgcgacttc ataaaaattt ccgcggttgg gctcgcagta
     7321 gcggctggca tgcgcgttgc gcaaggcgag gaagttgggg cttccagcgc caccaaaggt
     7381 gagcatcaat gggtaatggt gatcgaccaa tccaagtgta ctggctgtga acaatgcaac
     7441 ctggcttgcc acgcccataa tgatatcaat cccgaattgc agtggaccaa agttcttgat
     7501 gcgggcgaag tcaacggcga aaaagtgtac ctgccccgtc catgcatgca gtgtgaacat
     7561 gccccatgtg tggaagtctg ccctgtgggt gcctcctact atcgtgatga tggcattgtc
     7621 atgatggatt acgacaaatg cattggctgt cgctactgca tggtggcctg cccgtacgat
     7681 gcccgtgcct tcaattggaa ggaattcaca ggcccgaacc ccgccgtgcc cgaatggggc
     7741 gagcccgaag tagatcgtcg tccacgcggt gtaccggaga aatgctcctt ctgctacaac
     7801 cgcattgatc gcggcatgtc tgtgggtctt gtccccggtg tggatgcgga agcaaccccg
     7861 gcctgcgttg tcacctgccc aaccggcgcc cgcatcttcg gtgacctgaa cgacccgcat
     7921 tcaaacgtca gccaggtctt gaagaagcac ccatcctatc gcctgcgcga aaacctggga
     7981 accaatccac gtgtttatta cctgcccgta cgagaagacg gcgttgtgga ggactaacat
     8041 gaaaaccatc ctgaaaacca ttcgcgaaaa catctttacc ccgtggggat tgtggctggg
     8101 ctttctcggt atgttcctgg ttgcggcttt ggtggcgggt ctctcggtct tctggtttgg
     8161 tctttccctc accaacctta ccgacatcgt cccctggggt ttgtggatca caattgatct
     8221 ttcctccatc gctgtggcgg caggcgcatt cagcctttgt gccatcgtct acctctttgg
     8281 tttcaaacgc tacgaaccca ttgcacgcac agccactttc atcggtttta ccgggtatag
     8341 catggctatg atggctcttc tgctcgatat cggccgcccg gaccgtttct ggaaatcctt
     8401 cgtctattgg aacacccatt ccctgctttg ggaagtgacc atgtgcgtag gcctgtactt
     8461 taccgtgctg gtgctggaag tgctgcccat tgtggcttcc tttgactggc tgcgcaagaa
     8521 attcccgcgc attgccgaga agatggaaca tctgcatcat tatgcgccag tgcttgccat
     8581 attcggcctg ggactttcaa gcctgcatca gtcttcgctg ggagcagtgt atggcgtcat
     8641 caaagcccgc cccatttggt atcggccaga gatgtcggtg atgttcatgt tcacagccat
     8701 tcttggcggc gtggcactga cccttttcgc ctccatgctt tccgcccgcc tgacctccaa
     8761 agccaaagtg aatgacatcc tgatcgagcg cgtttcccaa gtccttggct atatgatgat
     8821 cgcctacctg tactggcgct tctgggattg gcttgcccaa acctacacct acgaacccgg
     8881 gcggactgag gcatttgaac tcctcaccaa agggactctc tccttcaact tttggttcgg
     8941 cgaaatgttg ttcggcgcgc tcattccgac cgtcatcctg ctctacaaac ccacacgttt
     9001 atctcccttc tggcggatgc ttggcctggc gatgatcgtg ggcggcgtgg ttgccttccg
     9061 tttcgatacc aacatcgtcg gtttccttgc ggtcattccc tacctgcccg gtgaagcggt
     9121 catctcctac acctcttatt cgccatcgct tgtagaatgg gctgccgggc ttggcatcat
     9181 tgcctttggc ctgctggcat tctccattgg tgtgaaatac ctgcgggtgg tggatcaccg
     9241 cctgaccagt gaagagtatg aaactgt
//
LOCUS       DAOQLC010000148         9154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_2955_length_9154_cov_22.628970, whole genome shotgun sequence.
ACCESSION   DAOQLC010000148 DAOQLC010000000
VERSION     DAOQLC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2955_length_9154_cov_22.628970"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PL000_18435"
     CDS             <1..1037
                     /locus_tag="PL000_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90916.1"
                     /translation="IAHLPLRAVQGSGEKQVRPRNDIINGLKTYTLWIIAFFIVYFIV
                     WPGMWVAPGKMLYEVYGNAFSYAFQGARLSVTQELQPSNFSLASAGLGVFDFLTSIAW
                     RTTPITWIGLALSIIFFFSKKEEVVSPLAKYLVASLLLEALACIGMFSLVQGRDQPHY
                     VLASYYGFEFAAAIGWALALRWLSLSFAWAGQRSAQIAFVAILLSAQAFPLLQNAPYF
                     FTYLNPIMTAAAGKPAPFFYGERMEQAAAYLAQQPDAKDQTALVYFGRSFSYYYPGKT
                     LLFKPILFDDKAQLIENLRESDYLVLYSGLEDRLPLLKELTPEHVIELYGRTYVEIYR
                     VADIPASFYK"
     gene            1081..>1735
                     /locus_tag="PL000_18440"
     CDS             1081..>1735
                     /locus_tag="PL000_18440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC90917.1"
                     /translation="MNLRKTFLVLLLLALTIAPRLSALDSFVTIDEPFWLSVGANYYY
                     ALGQRELENTVYEYHPAVTTMSVITAAFLVDFPDYRGLGQGYFDVDKEKFDPFIVANG
                     HDPMVLLVISRLFQVAIIILFVLIIFYLLSVLFGEEKAFFITAFVSSAPYFLGHSRVL
                     SHEAMVAFFVLASIFGLLAYLEFGRKGHYLLISGVSAALAQLTKSSAMAMFPVIVLML
                     "
     assembly_gap    1736..1835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1836..2805
                     /locus_tag="PL000_18445"
     CDS             <1836..2805
                     /locus_tag="PL000_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90918.1"
                     /translation="FGIWLGWLALAYFIFWPGMWVAPGKMLYEVYGNAFSYALQGARL
                     QVTQELQPSRFSLAAMGGSILNFILQVATKETPLSWIGIGLALFSLRRAGAAFEKRLM
                     LYLFLNAVMSILLFSAAQGRNSPHYIMSAHVSMDVIAALGWVSLSGLTLNQNARTNFQ
                     IKAGSVVFLLVMQLGSALFYHPYYYTYHNPLVVNAPLSDYGEGFEQAAAYLAQKPNAE
                     SLKVLSFRGRGPFSYFFSGETIIFNPLFLEEPGMVSMIERLEQADYLVINDALRPRTP
                     RTELFVSALDGVTPEHSIFINGVSTIRIYRMADLPASFYEVLSNAR"
     gene            complement(2822..3790)
                     /locus_tag="PL000_18450"
     CDS             complement(2822..3790)
                     /locus_tag="PL000_18450"
                     /inference="COORDINATES: protein motif:HMM:NF025600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HNC90919.1"
                     /translation="MSITRRDFLRLSAYGFGTAFLAACGFRPGDNPPPLNPTELPALS
                     PTVTSQALKELGFEAFADQLKVYQDAQYLYVESDGMPNHPMMIGIKSWQQQVPLPQPF
                     TGSNAWKIPLHPVVAENPISAKTDLYRGAIALAVNGVPIFNALNNRGDDAYLYGELDE
                     FGGHAGRADDYHYHTAPLHLSELVGMDKPIAYALDGFPIYGLTEPDGSAVTDLDEFNG
                     HFAADGSYHYHGTKTYPYINGGMRGVVTVRDDQIEPQPHMTPIREALQPLQGATITNF
                     TELDSSHYSLEYQINGQKYYVNYGFEGNTYTFEFVDASGKKTVETY"
     gene            complement(3794..4369)
                     /locus_tag="PL000_18455"
     CDS             complement(3794..4369)
                     /locus_tag="PL000_18455"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNC90920.1"
                     /translation="MKTNIFSLFLIFLLMGCSLAPGDPPAPPDTTPVIDPVSSNGTFL
                     LTSPEVSEGGALPREFTCDGSSATLPLAWSGAPTGTKSFAVVMHHIPGPGDSHWYWVV
                     YNIPADILSLEKNSTGIGTLGTNSVNGQTEYAPPCSKGPGEKVYTYTVYALSAEPQIS
                     LSASQVSREVLLDAIQNITLASAALNVTYTR"
     gene            complement(4373..5110)
                     /locus_tag="PL000_18460"
     CDS             complement(4373..5110)
                     /locus_tag="PL000_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90921.1"
                     /translation="MKRTSLFTLTTLLIVLTLAACRGAPPPPDQTTNTQPVPPTSDGS
                     VNTAGMVNIKADVWADNWFAFYLADQLIKEDSVSITTERSFNAETFTFAASYPMTFNF
                     IAKDFKENDTGLEYIGTNRQQMGDGGLIAQFINADTGEVIAVTDSNWMCTVIHEAPLD
                     KACETSANPVAGEMPCGFTSLEEPANWMDAAFDTSAWAKATIYSAGQVDPKDGFEQIA
                     WNPSAQFIWGPDLETNNTLLCKLTING"
     gene            complement(5191..7068)
                     /locus_tag="PL000_18465"
     CDS             complement(5191..7068)
                     /locus_tag="PL000_18465"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC90922.1"
                     /translation="MKTRYIPLLLFALLFTALVPISNTQAATLAYTVVDTAQTKCYNA
                     TGEIACPSSGSSFYGQDAQTTGTAPSYTISADGLTVADNNTNLTWVRKPDTNNDNSLT
                     EADKVTYSGASSACSSKASANYGGYSDWRVPTIKELYSLMDFRGTDPSGLSGSDTSGL
                     TPFIDTDYFYFVYGDTSAGARIIDAQYWSSTAYIAPSNDNKVFGVNFADGRIKGYPST
                     MAKQFIRCVRGDSYGVNSFVNNGDGTVTDNSSGLMWSQSDSASAMNWQEALAWVQTKN
                     AENYLGHNDWRLPNAKELQSILDYSHSPDTSNSAAINSVFNATQITNEGGAADYPSYW
                     SNTTHATYNGSGGFAVYVSFGRALGWMQLQGQSCYTLVDVHGAGAQRSDPKSGSSSSY
                     YLGTSCNGGSAYGHGPQGDIIRVDNFVRLVRDAVTSSTTFTDVPETHELYPYIEALYQ
                     AGYTAGCSTSPMMFCPDQILDRAQSSVFMLRGAMGSAYTPPAAPWNTFVNDDWTGYEW
                     AQAWAEGMFQEGLTAGCQTSPLMFCPETQLSRVEASVFGLRMKYGVDYLPPTASGTLL
                     ADMTDTSYWGTAWAEQAYQDGLLPACGTQNGKPMFCPNDLLTRGWGAYLIVKAKNIPT
                     P"
     gene            complement(7098..8990)
                     /locus_tag="PL000_18470"
     CDS             complement(7098..8990)
                     /locus_tag="PL000_18470"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HNC90923.1"
                     /translation="MNTLAKTFTTSLLVFLVGCQSLNPAPAQNTSNVLIEDAQANLLE
                     TTDESNSSRETESLDGIGNNTANPTWGSVNQPLLRFAAADYEDGNNSPAGADRPSPRL
                     ISNTFSVSPEEGIANDRDFTAFVYAWGQFLDHDIDLTDAASPKEAFPIVVPTGDAWFD
                     PANTGTMTIPVSRSNYDPNLSGPRQQTNSITAFIDGSQIYGVDPERAAALREFVGGRL
                     RTSTGNLPPLNTEGFPNANDAHRVDDSQLFLAGDVRANENPELLSLHTLFLREHNRIA
                     AEAAERHPDWTDEELYQFARRKVIAELQKITYDEFLPAILGPNAIPPYRGYQPEVNPG
                     IANEFSTAAFRFGHSMLGDDIEFLDNDGNENREAMPLRDSFFNPTVLHETDIDPILKY
                     LASDNAQEIDPIVVDDVRNFLFGAPGQGGFDLASLNIQRGRDHGLADYNTTRAAYGLE
                     RITSFEQITSDKALQASLASTYGSVDNIDLWIGGLAEDHLPNSSLGQTFTAILVDQFS
                     RLRDGDRFWYQASLSNRIVNDVQNTTLADIIRLNTDLTKLQADVFFFNESTVIVDAEA
                     PDQQNGQPNNGGSNGHQPPQAAINACSGLAENSSCSFIGRNQTTVNGTCLIVPSSTLA
                     CVPTRP"
BASE COUNT         1785 a         2118 c         2803 g         2348 t
ORIGIN      
        1 agattgctca cctgcccttg cgggcggtgc aagggtcggg cgaaaaacaa gtgcgccctc
       61 gcaatgacat tatcaacgga ctcaaaacct acaccctgtg gattatcgcc ttcttcattg
      121 tctacttcat cgtgtggccg gggatgtggg tggcaccggg caagatgctc tacgaagtgt
      181 acggaaatgc ctttagctat gcctttcagg gagcgcggct ctccgttaca caagaactac
      241 agccttccaa tttcagcctt gcctcggcag ggctcggcgt ttttgacttc ctcacttcca
      301 ttgcatggcg caccaccccc atcacatgga tcggacttgc actttcaatt atttttttct
      361 tttccaaaaa agaggaggtt gtttctccgc ttgccaaata tctggttgcc tccctgcttc
      421 tcgaagccct tgcctgcatt ggcatgttct ccctcgtgca gggacgtgac cagccgcatt
      481 atgtccttgc ttcctattat ggtttcgagt ttgccgctgc aatcggctgg gcattggctt
      541 tgcgctggct cagcctgtct tttgcatggg caggccagag atcagcgcag atcgcttttg
      601 ttgcgattct cttgagcgca caggcttttc cgctattgca aaatgcaccg tattttttca
      661 cctacctgaa tccgatcatg acggctgcgg ctggaaagcc tgctcccttt ttctatggcg
      721 aacgcatgga acaggccgcc gcctacctcg cgcaacaacc cgatgccaaa gaccagaccg
      781 ccctcgttta ttttggccga agtttcagct attactatcc cgggaagacc ctgctattca
      841 aaccaatcct tttcgatgac aaagcccaat tgatcgagaa tttgcgcgaa tcagattacc
      901 tcgtcctgta ttcaggcctc gaagaccgcc tgccgctgct caaagagttg actcctgaac
      961 atgtcatcga gttgtatggc cgaacctacg tggagatcta tcgcgtagcg gatattcccg
     1021 cgagttttta caagtgacag tcacctcaaa ggtgactgtc acttatgacg gacacaaccc
     1081 atgaacctcc gtaaaacatt ccttgtcctc ctcctgcttg ctttgaccat tgccccgcgt
     1141 ctttcggcgc tggattcttt cgtcaccatt gacgagcctt tctggctctc ggtgggtgcg
     1201 aattattact atgccctcgg tcagcgtgag ttggaaaata cggtgtatga atatcaccct
     1261 gctgtgacga cgatgtcggt catcacggcc gccttcctgg tggatttccc cgattaccgc
     1321 gggttggggc agggatattt cgatgtggat aaagagaagt tcgatccgtt catcgttgcc
     1381 aatggacatg acccgatggt tttgctggtc atcagccgcc tctttcaggt ggcgatcatc
     1441 attttattcg tgttgatcat tttttatctg ctttcggttt tgttcgggga ggaaaaggcg
     1501 ttttttatca cggcttttgt ttccagtgcg ccatattttc tggggcattc gcgggtgttg
     1561 agccatgaag cgatggtggc gttctttgta ctggcttcga tctttgggtt gctggcctat
     1621 ctggaattcg ggcgcaaggg acattacctt ttgatctcgg gtgtttctgc cgcgctggca
     1681 cagctgacaa aatcttcagc gatggcaatg ttcccggtca ttgtgttgat gctggnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntttcg gcatttggtt gggctggctg
     1861 gccctggcgt attttatttt ttggcccggt atgtgggtgg caccgggcaa aatgctttac
     1921 gaggtatatg gcaatgcctt cagttatgcc ttgcaaggcg cacgcctgca agtgacccag
     1981 gaattgcagc cttcgcgttt cagccttgcg gcaatgggcg gatcgatctt gaatttcatt
     2041 ttgcaggtgg cgacgaaaga gacaccgctt tcatggatcg ggatcggttt agcgctcttt
     2101 tccttgcgga gagcgggtgc ggccttcgag aaaagactca tgctttatct ttttctgaat
     2161 gcggtcatgt ccattttgct gttcagcgcg gcgcagggac ggaattcgcc gcactacatc
     2221 atgtcggcgc atgtgagcat ggatgtgatt gcggcgctgg gttgggtctc tctttcaggg
     2281 ttgaccctga atcagaatgc ccgaaccaat tttcagatta aggcaggaag cgtggtcttc
     2341 ctcctcgtga tgcagttggg cagtgcgctg ttttatcatc cgtattatta cacgtaccat
     2401 aatccgcttg tggtgaatgc gccgctttca gattatggcg aaggctttga gcaggccgcg
     2461 gcgtatttgg cacagaagcc gaatgcagag tcgttgaagg tactgtcgtt tcgcgggcgc
     2521 gggccatttt cttatttctt ttcgggggag accatcattt tcaatccgct gtttttggag
     2581 gagcctggca tggtttcgat gatcgagcgg ttggagcagg cggattatct ggtgatcaat
     2641 gatgcgttga ggccgcgtac accacgaacg gaattattcg taagcgcctt ggacggtgtg
     2701 acgcccgagc attcgatttt catcaacggg gtttcgacga tccgaattta tcgcatggct
     2761 gacctgccag catcgtttta tgaagtatta agtaacgcga gataatctct cgcgttacgg
     2821 tttaataggt ttccactgtt ttctttcctg aagcatccac aaattcgaac gtgtaggtat
     2881 ttccctcgaa cccataattg acgtaatact tttgcccgtt gatctggtat tccagcgaat
     2941 agtggctgga atccagttcg gtgaagttgg tgatggtggc accttgcagg ggttgcagcg
     3001 cctcgcggat cggggtcata tgcggctggg gttcgatctg gtcatcgcgg acggtgacca
     3061 ctccgcgcat gccgccgttg atgtagggat aggtcttcgt gccgtgatag tgataactgc
     3121 cgtctgcggc gaagtgaccg ttgaattcgt cgaggtcggt gacggcggag ccatcgggtt
     3181 cggtgagccc gtagatggga aagccatcga gggcgtaggc gatgggctta tccatgccga
     3241 caagttcgga aagatgcagt ggggcagtgt gatagtgata atcgtcggcg cggcctgcgt
     3301 ggccgccgaa ttcatccaat tcaccataga ggtaggcatc gtcgccgcga ttgttgagcg
     3361 cgttgaaaat tgggacgccg ttcacagcga gggcaatggc gccgcgatac aggtcggtct
     3421 ttgccgagat gggattctct gcaacaacgg ggtggagggg aattttccat gcgttgctgc
     3481 cagtgaaggg ttggggcagt ggcacttgtt gctgccagct tttgattccg atcatcattg
     3541 ggtggttggg cattccatcg gattcgacat aaagatattg ggcatcctga tacaccttca
     3601 attgatcggc gaaggcttcg aagccgagtt ctttcaaggc ttgtgaagtg accgttggcg
     3661 acagggcagg cagttcggtt gggttgagcg gagggggatt gtccccgggt ctgaatccgc
     3721 aggcggcgag gaaggcggtg ccgaatccgt aggcggagag tcttagaaaa tcgcggcgcg
     3781 tgatggacat gagttacctc gtgtaggtta cattcaaagc agcgctggcg agggtgatat
     3841 tttgaatcgc atcgagcaat acctcgcggc tgacctgtga agcggagaga gaaatctgtg
     3901 gttcggcgga aagcgcgtag acggtgtagg tgtaaacttt ttcgccaggg ccttttgagc
     3961 agggtggcgc gtattcggtt tggccgttga cactgttggt tccgagtgtg ccaatgcccg
     4021 tgctattctt ttcaagactc agaatatcgg cgggaatgtt gtaaacaacc caataccaat
     4081 gactatcacc aggtccgggg atgtgatgca tgacgacagc gaaacttttt gtccctgtgg
     4141 gtgctccgct ccacgcaagg ggcagggtgg cgcttgaacc atcgcaggtg aattccctgg
     4201 gcagggcacc gccttcggat acttctggac tggtgagtag gaatgtgcca tttgatgaga
     4261 cagggtcgat cactggagtt gtgtcgggcg gagcaggcgg gtcaccgggg gcgagcgagc
     4321 aacccatcag caggaagatg agaaataacg aaaatatatt tgttttcatg cgtcatccat
     4381 tgatcgtcaa tttgcacaga agcgtattgt tcgtttcaag gtcgggaccc cagatgaatt
     4441 gcgcagatgg attccacgcg atctgctcga atccatcctt cgggtcaacc tgcccagcgg
     4501 aatagatagt ggctttggcc caggcagacg tgtcgaaggc ggcatccatc cagtttgcag
     4561 gttcttccaa agaagtgaat ccacagggca tttcgcctgc cacagggttg gcggatgttt
     4621 cgcaggcctt atccagcggc gcttcgtgga tgacggtaca catccagttt gaatcggtga
     4681 cggcgatcac ctcgcctgtg tcggcgttga tgaattgcgc gatcaaaccg ccatcgccca
     4741 tttgctggcg attggtgccg atgtattcga gacctgtgtc attttctttg aaatctttgg
     4801 cgatgaaatt aaaggtcatc gggtaggatg cggcaaaggt gaaagtctct gcattgaagg
     4861 agcgttcggt cgtaattgaa actgaatctt ctttaatgag ttggtcagcc agatagaaag
     4921 caaaccaatt gtcagcccag acatcggctt tgatgttaac catgcccgcc gtgttgacgg
     4981 atccatctga cgtgggtggg actggctgtg tgttggtcgt ttggtcgggg ggtggcggtg
     5041 cgccgcgaca ggcggccagt gtgagcacga tcagcagggt ggtcaatgtg aaaagcgatg
     5101 ttcgtttcat gatggagagt ccttttgagt tggttttctt tggctacact cgcatagatt
     5161 tcaatgcgag cgtagccaaa gtatatgatt ttatggggtt ggaatgtttt tggctttgac
     5221 gatcaaatag gcgccccatc cgcgggtgag gaggtcattt gggcagaaca tgggtttgcc
     5281 gttttgggtt ccacaggcgg ggagcaagcc atcttgatag gcctgttcag cccaggcggt
     5341 accccaataa ctggtgtcgg tcatatcggc gagcagggtt ccactagccg tagggggcag
     5401 gtagtccacg ccatatttca tccgcaaacc gaagacgctg gcttccacac gggagagttg
     5461 ggtttcaggg cagaacatga gcggactcgt ctggcagcct gctgtgagtc cttcctgaaa
     5521 catgccttcg gcccaggctt gcgcccattc ataacccgtc caatcgtcgt tgacaaaggt
     5581 attccacggg gcggcgggcg gggtgtaggc agagcccatt gcgccacgca acatgaacac
     5641 ggaagattga gcacggtcga gaatttggtc ggggcagaac atcatggggc tggtgctgca
     5701 gcctgcggta tagcctgcct gatacaaggc ttcgatgtag ggatagagtt catgcgtttc
     5761 aggaacatcg gtgaaggtgg tgctggaggt cactgcatcg cgcacgagac ggacaaaatt
     5821 atctacgcgg atgatgtcac cttgcggtcc gtgcccgtaa gcagagccgc cattgcagct
     5881 tgtgccgaga taatagcttg atgaactgcc gctcttcggg tcgctgcgtt gtgcgcctgc
     5941 accgtgaaca tctacaaggg tgtagcagga ttgtccttgc aattgcatcc agccgagggc
     6001 gcggccaaag gaaacgtaga ccgcgaatcc gccactgccg ttgtaggtgg catgggtggt
     6061 gttggaccaa taagatggat aatctgccgc gccgccttcg ttggtgatct gtgtggcgtt
     6121 gaaaacggaa ttgatcgcgg cggagttgct tgtatcgggt gaatggctgt aatcgagaat
     6181 gctttgtaat tccttggcat tgggcaggcg ccagtcgttg tgaccgagat aattttcagc
     6241 attcttcgtc tgcacccagg ccagggcttc ctgccagttc atggcgctgg cactgtcgct
     6301 ttgtgaccac atcagtccgc tggaattatc ggtgaccgtt ccatcgccgt tgttcacgaa
     6361 actgttgact ccgtagctgt cgccgcgcac acagcggatg aattgctttg ccatcgtgct
     6421 gggatagcct ttgatgcgtc catcggcaaa gttgacgccg aagaccttgt tgtcgttgga
     6481 tggtgcgata taagccgtgc tcgaccagta ttgggcatca atgatgcgcg cacccgccga
     6541 cgtatcgccg tagacgaaat agaaataatc ggtgtcaatg aacggggtaa ggcctgaagt
     6601 gtcggagcct gagagcccgc tggggtcggt accgcggaaa tccatcagcg agtacagctc
     6661 tttgatggtg ggcacgcgcc agtcactgta gccgccgtag ttggcagatg ctttgctgct
     6721 gcaggccgag gatgcgcctg aataggtcac cttgtccgct tcggtgaggg agttgtcgtt
     6781 gttggtatcg ggcttgcgga cccaggtcag gtttgtgttg ttgtcggcca cggtcaaacc
     6841 gtcagcgctg atggtgtagc tcggggccgt acccgttgtc tgtgcgtcct gaccgtagaa
     6901 ggaggatccg cttgatgggc aggcgatttc tccagtcgcg ttgtagcatt tggtttgggc
     6961 ggtatccacc actgtgtagg caagggttgc cgcctgtgtg ttggaaatgg gaacaagcgc
     7021 ggtgaagagc agggcaaata gaagcagagg aatgtagcgg gttttcattt cattctcctt
     7081 tgatgattct cttagtgcta tggccttgtg gggacacaag caagcgttga gcttgggacg
     7141 atgagacagg taccgttgac ggtggtttga ttcctgccga tgaaagagca ggaggagttt
     7201 tcagcgaggc ctgagcaggc gttgatcgcg gcttgcggag gctggtgtcc gttggatccg
     7261 ccgttgtttg gttgtccgtt ttgctgatcg ggcgcttcag catccacaat gacggtggat
     7321 tcgttgaaga agaagacgtc tgcctgaagt ttggtcaggt cggtgttgag gcggatgata
     7381 tcggcaaggg tggtgttttg cacatcgttg acgattcgat tcgacaggga agcctgatac
     7441 cagaaacggt caccatcacg cagacggctg aactgatcga cgaggattgc ggtgaaggtt
     7501 tggccgaggc tggagttggg caggtggtct tcggcgaggc cgccgatcca caggtcaatg
     7561 ttgtccacgc tgccgtaggt ggaggcgagg gaggcttgca gggctttgtc tgaggtgatt
     7621 tgctcgaagc tggtgatgcg ttcgaggccg taggcggcac gggtggtgtt gtagtcggcg
     7681 agtccgtggt cgcgtccacg ttggatgttg agggaggcga ggtcgaatcc gccttgaccg
     7741 ggggcgccaa agaggaaatt gcggacgtca tccacgacga tggggtcaat ttcctgggcg
     7801 ttgtcagagg cgagatattt cagaatgggg tcaatatctg tttcgtggag caccgtgggg
     7861 ttgaagaagg aatcgcgcag tggcatggct tcgcgatttt cgttcccgtc attgtcgaga
     7921 aattcgatgt catcgcccaa catgctgtga ccaaaacgaa aggccgcggt ggaaaattca
     7981 ttggcgatgc cggggttcac ttctggttgg tagccgcggt aggggggtat ggcattggga
     8041 ccaaggatgg cggggaggaa ttcgtcgtag gtgatctttt gcaattcggc aatgaccttg
     8101 cggcgcgcaa attgatacag ttcctcgtcg gtccaatcgg ggtggcgttc ggcggcttct
     8161 gcggcaatgc ggttgtgctc gcgcaggaag agcgtgtgca gggaaagcag ttcggggttt
     8221 tcgttggcgc ggacatcgcc tgcaagaaac agctggctgt catccacacg gtgggcatcg
     8281 ttggcattgg ggaagccttc ggtgttgagt ggcggcaggt tgcctgtgct ggtcctcagt
     8341 cgtccgccga cgaattcgcg caaggccgcc gcacgttcgg ggtctacgcc ataaatttgg
     8401 gagccatcaa tgaatgcggt gatggaattg gtctgctggc gcgggccgct gaggtttggg
     8461 tcgtagttcg agcgtgagac ggggattgtc atcgtacctg tgttggcggg gtcgaaccat
     8521 gcatcgcctg tgggaacgac aatggggaaa gcctctttcg gcgaggcggc gtcggtcagg
     8581 tcaatatcgt ggtcgaggaa ttgtccccaa gcgtagacga aggcggtgaa atcgcggtca
     8641 ttggcgatgc cttcttcggg gctgaccgag aatgtgttgc tgatcagcct cgggctggga
     8701 cgatccgcgc ctgcggggga gttgtttcca tcttcatagt ccgctgcggc gaagcgaagc
     8761 aggggctggt tgacgcttcc ccaggttggg ttggcggtgt tgtttccgat tccatccagc
     8821 gattcggttt cgcggctgga attggattcg tcggttgttt ccaataagtt tgcctgtgca
     8881 tcttcgatca gtacgttgga ggtgttttga gcgggggcag ggttgagaga ctggcacccg
     8941 acgaggaaga cgagcaggct ggtggtaaag gttttggcga gggtgttcat gttgggttcc
     9001 tttggtgttg aactattttt agattgaatt gacctcttgc ataagtaaag tgttgtagac
     9061 cacaaagtca ccaaggcacc aagctttttt tctttgagtc ttcgagactt tgtgcctcac
     9121 ctgcactggc gtgcggcgca agtgttgtgg taaa
//
LOCUS       DAOQLC010000149         8953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3048_length_8953_cov_26.957519, whole genome shotgun sequence.
ACCESSION   DAOQLC010000149 DAOQLC010000000
VERSION     DAOQLC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_8953_cov_26.957519"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PL000_18475"
     CDS             <1..1066
                     /locus_tag="PL000_18475"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC90924.1"
                     /translation="ITIPPMKTTHPLLDLARKSGNPVITGNQVTFVWKGKSAPCLITD
                     IHEWEDHPQKLTRTAPDLWTCSLDLHRDAYLEYAFYDPRTKKRIKDPLNKKTVYNGIG
                     QYNHFFYMTAASPTIYTAKRKEIPVGRITRHLVDASLMHEGGKRELHLYHPPVHEPVP
                     LLLVYDGTDYLKRAMLNNIVDNLINEKRIRPIAMAFLQNGDDHRSLEYACSDATLTAL
                     DQSILPFASQKLNLLSPKQTRGAFGVLGASFGGLMSMYTGLRMPDIFGKVICQSGVFE
                     LNSRDFVAVDLIRAKQSREIAIWMDIGRFDSLLEDNRRLQPLLRENGYNVTYREANAG
                     HNFTFWRNEIPMALEAMFPA"
     gene            1063..1521
                     /locus_tag="PL000_18480"
     CDS             1063..1521
                     /locus_tag="PL000_18480"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNC90925.1"
                     /translation="MILATLCYVKKDGHTLMIHRNKRPNDIHEGKWNGLGGKFEKGES
                     PEECVIREVWEESGLTLRTPRLHGTLTFANFKGNHWYVFVFTAHEFEGKLLQDVPEGS
                     LEWVPDEKILDLPLWDSDQVFLPWIEQGRFFSAKFNYDGERFINHAVAFY"
     gene            1686..2468
                     /locus_tag="PL000_18485"
     CDS             1686..2468
                     /locus_tag="PL000_18485"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC90926.1"
                     /translation="MKLETITRVPENQTHQTPLLFIHGMWHGAWCWDEFFLPFFAGHG
                     FHVTALSLRGHAGSEGNIRGSKIADYIHDVEQIAKTLPTPPALIGHSMGGFLTQKYLE
                     RNTAPAAVLLASNPYFGLWPTFLRMSAQRPLTLLKVLSQLRLYPVVENSEAARWTLFS
                     KDMPEEQVLKYHKKMNDESFRMFLDLLGLNLAHPQKIKTPLLILGAEFDNAITAGQVH
                     ATARGYGLQAEIFPNMAHDMMVEAGWRSVAERILNWLNEKGI"
     gene            2471..4438
                     /locus_tag="PL000_18490"
     CDS             2471..4438
                     /locus_tag="PL000_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90927.1"
                     /translation="MKKTFIGIAVLVITTLACSTLMPAQSAAEPTVGATDVPAPGSGV
                     GLSGLDGFPCPDSDFTCVTLTVPLNHFDPSDSRTLEVVFGVLPATGERKGMFVTATGG
                     PGTAGLMSADGYTAVMDPAIPEHYDIVFFDQRGVGQSGGLQCTNAAAKYFLADFSTGT
                     PEEEQALLQTARSFANDCVTEAGNPDFLPYLGTAQAVEDLELFRQAMGDEKIWLYGES
                     YGTQYAQTYAAAHADHLAGLILDGTVDLTLTGLEYYTQQAAAFNSTLTLTLEACNADD
                     ICAESMGGGDAIAAYNKLSQRLKQGSIEFNFPLPSGGIANRAFTFSDLENSAASYMYS
                     ETLRMIFLRALAAYSRNEDIVPMARILYASLALDPETLIPKTDPSYSDALFYAVECQD
                     YSYFSGTPEERGTALMQAGNAVEQSLENFESIFYGDVPCLFWPNTPTENTRPAPLVAD
                     GVPTLVLGATADPATPVSNGIAVFRRLADGYLVTQNGGPHVTYGVGSACVDAIVTEFL
                     VNGQPPAERENTCEGVVADEFISLLPLDAKEFIDPLDALTMIEDEIYLLPEYYYWDYI
                     TPTSIGCPFGGVMTFEAADAGDSFSFDNCSFTNGFSLSGSGFNNLDDGTFAFDVTVSG
                     LKAGTLTYLHDSEGGRNVRGEYGGEAIDISR"
     gene            complement(4463..4960)
                     /locus_tag="PL000_18495"
     CDS             complement(4463..4960)
                     /locus_tag="PL000_18495"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNC90928.1"
                     /translation="MKDHILAALQEQFHQLEALLESLSEEQMTAPNFDEDWSVKDIVN
                     HLWGWQQISIARMKAGLLDREPEFPAWLVSFPGNWDENASQTNAWIYKNFHDQPWAEV
                     NKKWREGFLQLLDLGGRVSERYLLDGDRYVWLKGYSLAFILVASYEHHQEHLEKLVIW
                     LKNQQ"
     gene            complement(4991..5755)
                     /locus_tag="PL000_18500"
     CDS             complement(4991..5755)
                     /locus_tag="PL000_18500"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNC90929.1"
                     /translation="MNLRNAFSQLGLSKQLVIAHASLRAFGSVEASAVLDALLETTRG
                     VIMPTFTYKTMLNPEVGPPHNGITYGSEADLNKMAEPYRPDMPVDKLMGILPEVLRNH
                     PKARRSSHPIQSFAGINADAIVNAQTIYEPLAPIAALAERDGWVLLLGVDHRVNTSIH
                     YAEKLSGRMQFIRWALTPGRVVECPGFPGDSEGFNAIAPAVEKFTRRVEVGNALVQAV
                     PLRNLIHAVMDAIRDNPLALLCGRPDCERCEAVRWG"
     gene            complement(5807..6580)
                     /locus_tag="PL000_18505"
     CDS             complement(5807..6580)
                     /locus_tag="PL000_18505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNC90930.1"
                     /translation="MAYTTILTETRGRVGIVTLNRPQAMNAFNSTMLTELFDALEAFD
                     TDPAVGAMVVTGNEKVFAAGADIKEMAEASPFEMITQGRVEKWDRIRAIKKVVIAAVS
                     GWALGGGCEFAISCDMIVASESAKFGQPEITIGVIPGGGGTQRLARLLGKQLAMEMVI
                     NNRTLTAAEALQFGLANRVVPVEGYLDSAIAFAEEIASRAPMAVRMAKDAVNAAFETT
                     LTEGLRAEKRNFYPLFSTEDQKEGMKAFIEKRKANWKGK"
     gene            6676..7074
                     /locus_tag="PL000_18510"
     CDS             6676..7074
                     /locus_tag="PL000_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90931.1"
                     /translation="MQILLMIHSYVRWLIVLVAIAAVIKFALGWLRGGQFKGMDRGLA
                     SGFSGLIDLQATLGLILMLWSGFSGVGFPMFRIEHATTMVIAAVVAHLPARWKTAADK
                     TRFRNTLFCVLGALLLIYVGVMRLPGGWSR"
     gene            7185..7490
                     /locus_tag="PL000_18515"
     CDS             7185..7490
                     /locus_tag="PL000_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNC90932.1"
                     /translation="MENKLYVGNLPYATTEDELKAHFSQAGTVTSVALIKDRATGRAK
                     GFGFVEMSTADEAQKAISMFNGQNFMGRSITVNVARPPENRPRNFGDRNRGGGQNRN"
     gene            7560..8141
                     /locus_tag="PL000_18520"
     CDS             7560..8141
                     /locus_tag="PL000_18520"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HNC90933.1"
                     /translation="MKRIILLGPILFAIVVSTLTLVKYEFLRGLGWHPIHDPTFDWPS
                     GLALGPYGWIMTTTFIISGSLMTLFAARLFLDLEPVRTSQIGCTLLAFSGLALAALAF
                     TTDPTLRTTPATWHGRLHDLSFVLLGLTLFPAMIVLGFAFRKSERWKPYAFYTWLTIA
                     LAAPAFAIKGAAFYVFLFAILLWNEIIALKLTD"
     gene            8230..8787
                     /locus_tag="PL000_18525"
     CDS             8230..8787
                     /locus_tag="PL000_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90934.1"
                     /translation="MRIARAIKTDQETIKRFLDVLGGGSVALGNSRLAQVSFFVLGHS
                     FIQEFIEGIFRKKEEYLINALEDGGFPTEGGPIGSLKADQKKCLEAAEHLLHASQQWQ
                     DGDEIARSEVGWAVSEFNNSCRQRLDRMKNLIIPLLEQTFSLEEDQKMAEEITNMAFE
                     NLPKDTPDKYIKVIEKLEEQLSDWK"
BASE COUNT         2137 a         2588 c         2184 g         2044 t
ORIGIN      
        1 cattacaatt ccccccatga aaaccaccca tccccttctc gaccttgccc gtaaaagcgg
       61 aaaccctgtc atcacaggaa atcaggtcac ctttgtatgg aaagggaaat ccgcgccgtg
      121 tttgatcact gacatccacg aatgggagga tcatccgcaa aagctgaccc gcaccgcccc
      181 cgacctgtgg acctgctcac tcgacctgca tcgagatgcc tacctcgaat acgcctttta
      241 cgacccgcgc acaaaaaaac gcatcaaaga cccattgaat aaaaaaacgg tctacaacgg
      301 catcgggcaa tacaaccact tcttttacat gaccgccgca agccccacca tctacaccgc
      361 caaacgcaaa gagatccccg tcggcaggat cacccgtcac cttgtggatg catcgttgat
      421 gcacgaaggc ggcaaacgcg aactgcacct ctaccaccca cccgtccacg aacctgtgcc
      481 gctcctgctg gtgtacgacg gcaccgacta cctcaaacgc gccatgctca acaacatcgt
      541 ggataacctc atcaacgaaa aacgcatccg cccgatcgcg atggccttcc tgcaaaatgg
      601 agatgaccac cgcagcctgg aatacgcctg ttcagatgca accctgaccg ccctcgatca
      661 atccatcctg ccatttgcca gccagaaatt gaatctactc tccccgaaac agacccgcgg
      721 cgcatttggc gtattgggcg catccttcgg cggattaatg tccatgtaca cggggctgcg
      781 catgcccgat atctttggga aggtcatctg tcagtcagga gtcttcgaat taaacagccg
      841 cgacttcgtc gccgtggacc tgatccgcgc gaagcaatcc cgcgagatcg cgatctggat
      901 ggatattggc cgcttcgaca gtctgctcga agataaccgc cgacttcaac cgctcttgag
      961 agagaacggc tacaacgtca cctaccgcga agcgaacgca ggccacaact tcaccttctg
     1021 gcggaatgaa attccaatgg cattggaggc aatgttccct gcatgatcct cgcaacctta
     1081 tgctacgtca aaaaagatgg gcacaccctg atgatccatc gcaacaaacg ccccaacgac
     1141 atccacgaag gcaagtggaa tggactcggc ggcaaatttg aaaaaggcga atcccccgaa
     1201 gaatgtgtca ttcgcgaagt gtgggaagaa tcggggctga ccctccgcac accgcgcctg
     1261 catggcaccc tcacctttgc caatttcaaa ggcaatcact ggtacgtctt tgttttcaca
     1321 gcgcatgaat ttgaaggcaa acttctgcag gatgtacccg aaggcagcct ggaatgggtc
     1381 cccgacgaaa agatcctcga cctcccgctt tgggatagcg accaggtctt cctgccgtgg
     1441 atcgaacagg gacgattctt ctccgccaaa ttcaactacg acggcgaacg cttcatcaac
     1501 cacgctgtgg cattttatta actgatgtta cgcaggactg caagatttat cctgccattc
     1561 gcttgttttc ccaacttgca ggcgcaaagt gaactttgcg gcacacccca accccatccc
     1621 attcaaactg atacaatgga agcatacatt cgacggacga ttggccatgt aagaggagag
     1681 cagccatgaa actcgaaacc atcactcgtg ttcccgaaaa tcaaacccat caaacgcccc
     1741 tgctttttat ccacggcatg tggcatggcg catggtgttg ggatgaattc ttcctgccct
     1801 tctttgcagg gcatggattt catgtcacag cactcagcct gcgcggacat gcaggcagtg
     1861 aagggaatat ccgtggaagc aaaattgcgg attacatcca tgatgtggag cagatcgcaa
     1921 aaacgcttcc gactccccct gccctgatcg gccattcgat gggcggattt ctcacccaga
     1981 aatatctcga aagaaataca gcgcctgccg ccgtgctgct ggcatcgaat ccatactttg
     2041 gattgtggcc aacctttctg cgcatgtccg cacagcgccc gttgaccttg ctgaaggtac
     2101 tctcgcaact gcgtttgtat ccagtggtgg aaaattccga agccgcccgc tggaccctgt
     2161 tttcaaaaga catgcccgaa gaacaggttt tgaaatatca caaaaagatg aacgatgaat
     2221 cttttcgcat gtttcttgac ctgctcgggc tgaatctggc tcatcctcaa aaaatcaaaa
     2281 cgcccctgct catcctcggt gcggaatttg acaacgccat caccgcgggt caggtccatg
     2341 ccacggcgcg gggatatggg ctgcaggcag agatcttccc gaacatggca cacgacatga
     2401 tggtggaggc gggttggaga tcggtagccg aaagaatcct gaactggttg aacgaaaaag
     2461 gaatatgacc atgaaaaaaa catttatcgg tattgccgtg ctggtcatca ccacgctggc
     2521 ctgttccacg ctgatgcccg cccaaagtgc cgcagaacca accgtcggcg caacggatgt
     2581 tcctgcgcca ggatcaggcg tcggcttaag tgggctcgat gggtttcctt gtccagatag
     2641 tgatttcacc tgcgttacat taaccgtgcc gctgaatcat ttcgacccat ccgattcgcg
     2701 cacgctggag gttgtctttg gtgttttgcc tgcaacgggt gaacgcaaag gcatgttcgt
     2761 cactgccact ggcgggcctg gcacggcagg tttgatgagc gcagatggat acaccgctgt
     2821 gatggatccc gccattcccg agcattacga cattgttttc tttgatcagc gtggcgtggg
     2881 gcaatcgggc gggttgcaat gcacaaatgc ggccgcaaaa tatttcctgg ccgatttttc
     2941 cacaggcaca cccgaagagg agcaggcgct tttgcagaca gcacgcagct ttgcaaatga
     3001 ctgtgtcacc gaagcgggca accccgattt tcttccatac cttggcaccg ctcaagccgt
     3061 ggaagatctg gagcttttca ggcaggcgat gggcgatgaa aagatctggt tgtatggcga
     3121 aagttatggc acgcaatatg cgcagaccta cgccgcggca cacgccgacc atctggcggg
     3181 gttgatcctt gatggcacgg tggatctcac gctgacaggg ttggagtatt acacccaaca
     3241 agcggcagcc ttcaacagca cgctgaccct gaccctcgaa gcctgcaacg ccgatgatat
     3301 ctgcgcagag tcgatgggcg gcggtgatgc cattgcagcc tacaacaaac tttcacaacg
     3361 attgaaacaa ggcagcattg aattcaactt ccccctgcct tcgggcggca ttgcaaaccg
     3421 cgccttcact ttttcagatc ttgaaaattc agcagccagc tacatgtatt ccgaaacatt
     3481 gcgcatgatc ttcctgcgcg cgctggcggc gtactcacgc aatgaagata ttgtgcccat
     3541 ggcgcgcatc ctgtatgcat cgctggcgct cgacccagaa acactcatcc ccaaaaccga
     3601 cccttcctat tcagatgcgt tgttttatgc agtggaatgt caggattaca gttatttcag
     3661 cggcacaccc gaagaacgcg gcacggcttt gatgcaggcg ggaaatgcgg ttgaacaatc
     3721 gctggaaaat ttcgaatcga tcttttatgg ggatgtgccc tgtcttttct ggccaaacac
     3781 acccaccgaa aacacccgcc ccgcgccgct tgtggctgat ggtgtaccca cattggtcct
     3841 tggcgcaacc gccgaccccg ccacaccggt ctcgaatggc attgcggtct tccgccgcct
     3901 tgctgatggc taccttgtga cacaaaatgg agggccgcac gtcacctatg gtgttggctc
     3961 ggcctgcgtg gatgcgatcg tgacggagtt tctggtgaac ggccaaccgc ccgcagagcg
     4021 cgaaaacacc tgcgagggtg tggtggccga tgaattcatt tcattactgc cgttggatgc
     4081 aaaagagttt attgacccgc tcgatgcact gaccatgatc gaagatgaga tctatcttct
     4141 tcctgaatac tattactggg attacatcac accgacctcg atcggatgtc catttggcgg
     4201 cgtgatgacc tttgaagccg cggatgcagg cgattctttc tccttcgaca actgctcctt
     4261 cacaaatggc ttttccctca gcggcagtgg attcaataac ctggacgacg gcaccttcgc
     4321 tttcgatgta accgtcagcg gattgaaagc gggcacactc acctacctcc acgattcaga
     4381 aggcggacgc aacgtccgcg gggagtatgg cggcgaggct attgatattt caaggtagag
     4441 actgcctgct tgtacggcgg gtctattgct gattcttcag ccagatcacc aatttttcaa
     4501 gatgctcctg gtggtgttcg taagaagcca ccaggatgaa cgcaagcgaa tatcccttta
     4561 accatacata tctatcccca tcgagtaaat atctttctga aaccctcccg cccagatcaa
     4621 ggagttgaag aaaaccttcc ctccattttt tattcacctc cgcccacggt tggtcgtgaa
     4681 agttcttgta gatccaggca tttgtctggc ttgcgttttc atcccaattg cccgggaaac
     4741 ttacaagcca ggctgggaat tcaggctcac gatcgagcaa gcctgctttc atccttgcaa
     4801 ttgaaatctg ctgccagccc cacaagtgat taacgatatc tttgacagac cagtcctcat
     4861 cgaaatttgg ggcggtcatt tgttcttcgc tcaacgattc aagcaatgcc tccaactgat
     4921 ggaactgttc ctgcaacgcc gccaggatat gatctttcat gctcatgatg tgcctcattg
     4981 ggtcaaacca tcatccccag cggacggctt cacagcgctc gcaatcgggc ctgccgcaca
     5041 acaaagccag cggattatcc ctgatggcat ccatcacggc gtgaatcaga ttccgcaggg
     5101 gtacggcctg cacaagcgca ttgccaactt ccacacggcg ggtgaatttt tccacagcgg
     5161 gtgcaatggc attgaatccc tctgaatcac cgggaaagcc ggggcattcc accaccctgc
     5221 ccggggtcag cgcccagcgg ataaactgca ttcggcctga gagcttctca gcataatgga
     5281 tactcgtatt cacccgatga tccactccaa gtaacagcac ccagccatcc cgttcggcca
     5341 gagccgcaat gggcgccagc ggctcataaa tggtctgcgc gttgacgatc gcatctgcat
     5401 tgatccccgc aaaagactgg atcgggtgtg aagaacgcct cgccttcgga tggtttcgca
     5461 acacctcagg caggatgccc atcaacttat ccacgggcat atcagggcgg taaggttcgg
     5521 ccattttgtt caaatcggct tcactgccat aggtgattcc gttgtgcggc ggccccacct
     5581 ccggattcaa catggttttg taggtgaagg tcggcatgat cactccgcgc gtggtttcaa
     5641 gcagggcatc gagcacagcc gaggcctcca ccgaaccaaa ggcgcgtagt gaggcatgtg
     5701 caatgacaag ttgtttgctc aggcccaatt gtgaaaaagc atttcgcagg ttcattcgtt
     5761 tgacaatcag atggttggat ggttaagggt tcaaacagcg aacaggctac ttccctttcc
     5821 agttcgcctt gcgtttttca atgaaggctt tcatgccttc cttttgatct tccgttgaaa
     5881 acagcggata gaagttgcgt ttctcagccc gcagcccttc ggtcagtgtg gtctcgaaag
     5941 cggcgttgac ggcatccttc gccatgcgca ccgccatcgg tgcgcgcgaa gcgatctctt
     6001 ctgcaaaagc aatggcagaa tcgagatagc cttccacagg cacaacccga ttcgcgagcc
     6061 cgaactgcag ggcttcggcg gcggtcagtg tgcggttgtt gatgaccatt tccattgcaa
     6121 gttgttttcc gagcaaacgc gccagccgct gggttccgcc cccgccggga atcacaccga
     6181 tggtgatctc aggctgtcca aatttcgctg attcagaagc gacgatcata tcgcaggaga
     6241 tcgcgaattc gcacccgccg cccagcgccc accccgaaac cgccgcaatg accacctttt
     6301 tgatcgcgcg aatacggtcc catttttcaa cgcgaccctg tgtgatcatt tcaaaaggcg
     6361 aggcttctgc catttccttg atatctgcgc ccgcggcaaa gactttctcg ttgccggtca
     6421 ccaccatcgc accgacggcg gggtctgtat caaaggcttc cagcgcgtcg aacaactcgg
     6481 tcaacatggt ggaattgaaa gcgttcatcg cctgcgggcg attcagggtg acaatgccca
     6541 cccgtccgcg ggtttctgtc agaatggttg tgtatgccat gagtcctcca agtggattgg
     6601 gcatttccat gcccatgcag taaattataa gggatgaaag gccttgttgt ataatcgccc
     6661 actggagaac aaaacatgca aatcttatta atgatccatt cttatgtccg ttggttgatc
     6721 gtccttgttg ccattgcggc tgtcatcaaa tttgcactgg gctggctgcg cggcggccaa
     6781 ttcaaaggca tggaccgcgg tcttgcctct ggtttcagcg gcttgatcga cctgcaggcc
     6841 acactcggcc tcatcctcat gttatggtct ggttttagcg gtgtcggctt ccccatgttc
     6901 cgaattgagc atgccaccac catggtcatt gctgcggtag tggcgcatct gcctgcccgc
     6961 tggaaaaccg ccgcagacaa gacccgcttc cgcaacaccc tgttctgcgt cctcggcgca
     7021 ttgctgctga tctatgtcgg ggtgatgcga ctgcctggcg ggtggagcag gtaacggctg
     7081 cgtctgatcg gtcttttttc caaaaaatta ttctcagttt atattaatca tgataaactt
     7141 tgtcatgtcg ttcaggctgg aacgttgaca cacttggaga agagatggaa aacaagttat
     7201 atgtaggcaa cctgccctac gccaccactg aagatgaact caaagctcac ttcagtcagg
     7261 ccggaaccgt tacttctgtt gcgttgatca aagatcgtgc cactggccgc gccaagggtt
     7321 tcggctttgt cgaaatgtca acagccgacg aagcgcaaaa ggccatcagt atgttcaatg
     7381 gccagaactt tatggggcgc tccatcacgg ttaatgtcgc ccgcccgcct gaaaaccgtc
     7441 cgcgtaattt cggtgatcga aaccgcggcg gtggacaaaa ccgcaactag aaaaatctcc
     7501 agccctgaac ctgcatgaaa tcaacagccc cgaatgaacg gggctgttat cattaaggca
     7561 tgaaacgcat catccttctc ggccccatcc tctttgccat cgttgtttcc acgctcacac
     7621 tcgtcaaata cgaattcttg cgcggcctcg gctggcatcc catccacgac cccaccttcg
     7681 attggccaag cggcctcgct cttgggccct acggatggat catgaccacg accttcatca
     7741 tcagcggatc gctcatgacc ctcttcgccg cccgtctctt tctggacctt gaacctgtcc
     7801 gcacatctca aattggatgc acccttctgg cattctctgg tttagccctt gccgccctcg
     7861 cattcaccac cgacccaacc ctgcgcacca cacccgccac ctggcatgga cgtttgcacg
     7921 acctgtcttt tgtgctgctg ggcctcacgc tcttccccgc catgatcgtg cttgggtttg
     7981 ccttccgcaa aagcgaacga tggaaaccct atgccttcta tacctggctg accattgcgc
     8041 tggccgctcc cgccttcgcc atcaaaggcg cggcatttta tgttttcctt tttgcaattc
     8101 tgctttggaa tgaaatcata gccttgaaat tgaccgatta gaagtaggca ggccgaaaat
     8161 ttccgctctg ttttacggca tggtatactc cacaaaacat tacatcccaa aaaagaatca
     8221 gggaaagata tgcgaatcgc aagagccatc aaaaccgacc aggaaaccat caaacgtttc
     8281 ctcgatgtac tgggcggggg atccgttgcc cttggtaaca gcagactggc ccaggtcagt
     8341 tttttcgtgc ttggccacag tttcattcag gaattcatcg aaggtatctt tcgaaaaaag
     8401 gaagaatacc tcatcaacgc gctggaagat ggcggcttcc ccaccgaggg cggtcccatt
     8461 ggctcactaa aagctgacca gaaaaaatgc cttgaagcgg ccgagcacct tctgcacgca
     8521 tcacagcaat ggcaggatgg cgacgaaata gcgcgcagcg aagtgggttg ggcggtcagc
     8581 gaattcaaca attcgtgccg tcaacgcctt gaccgcatga agaatctgat catcccactg
     8641 ctggaacaaa ccttctccct tgaagaagat caaaaaatgg ctgaagagat caccaacatg
     8701 gccttcgaaa atcttcccaa agacacgcca gacaagtaca ttaaggtcat tgagaaactc
     8761 gaagagcagt tgagcgattg gaagtagcag taaataagtc atcactcaaa acaggtcaga
     8821 aactctgacc tgttttgtct ttaattgcct aaactctgga ttgccagcaa attatatata
     8881 atgtacaatc cgcgccaatc aaggaatcat tcccccatgc taaatctcaa cctcaaaacc
     8941 acccccttcc acg
//
LOCUS       DAOQLC010000150         8874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3094_length_8874_cov_17.245039, whole genome shotgun sequence.
ACCESSION   DAOQLC010000150 DAOQLC010000000
VERSION     DAOQLC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3094_length_8874_cov_17.245039"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..744
                     /locus_tag="PL000_18530"
     CDS             73..744
                     /locus_tag="PL000_18530"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90935.1"
                     /translation="MTITKIGGMEFYDVHFSYIDLEDGQEHFVNVPEQTGGGLIHEGR
                     PKPGIVHTIAMSGNEMPTIYRIEIQTLAGTGKLLVSGPLSREPVRIAFDYFKAHASRV
                     SGMIRPSEHDFHLHLVDLQGGGEPDVSTLASLIALSSGAIGRPVQSQLVIMGDMSLGG
                     VITPARNLAESLQVAFDAGAKRILLPMSSVGDIPTVPGELFAKFQTSFYSDPVDAVFK
                     AIGVE"
     gene            871..1734
                     /locus_tag="PL000_18535"
     CDS             871..1734
                     /locus_tag="PL000_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HNC90936.1"
                     /translation="MSEWNEIKRSHSKTNFPHLQLGNRFFSKKPCREAVGNIYKVNLK
                     KERKMKNMTKVWIMASMVSVNAWSGESVTSDLDKCITQKMISSTAKGAVTGALMGFLA
                     AKASGKDKEHERKAALIGAAAGGVFGYVKAWNKAVDDCKKEHPDWVVESDIKRSPNYD
                     AVVAEFNYKPPQKNSIAIVRPLQIPEPVKPGDTANIQAKFVILTPDGGEAKVKIDRKF
                     FVVADGQEEPLSFPGRDSEDRVVENGEQVDEFNLPIGKDVPVGTKVRVQYAISLNGAS
                     LVSQSGIIEVK"
     gene            1731..3062
                     /locus_tag="PL000_18540"
     CDS             1731..3062
                     /locus_tag="PL000_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNC90937.1"
                     /translation="MTMRTAIMRGVTLALAILLAGCTGYQSMPGYQRVYQGYQVPQNL
                     ADAVQARLRTEGISQAIIGRDSVGRLKLNGVYRDEDEVERAFIIVQSIVGIQSTSPFY
                     PENVQEKRWEREAGAALSDYFKRKKQAQSNVPGKKRALVIGINRFQSYPQITDIQGED
                     DARLMGIVLANYGYDVRSLLGERATKAKIESEIANLDKVLEPNDTLFIYISSHGTPPV
                     PTSGGGDERKMSIVAYDSGPLNSSMDRTGGALTIQRTSVSDKLVQRLAQRTSAMTRVI
                     IDTCYSGEILRDMSNVGSAYQDKMNGGGFEKASVSESSWTGASYTSKAIAFVDDGNSS
                     VGQASKTSEQVTRRNYSFITATSPGELSWGPPSTVGTFPDPTSQSELLRGSFFTQSFI
                     AYLKQFSGDVPRAFEEARIFTSKMVQQGPKQSQNPRRFTTIPADIDNLSKH"
     gene            3080..3949
                     /locus_tag="PL000_18545"
     CDS             3080..3949
                     /locus_tag="PL000_18545"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNC90938.1"
                     /translation="MNHCSLNKAILIAFAVMSVGVHAEEPTYSAKALFFGEDGDMKTV
                     ATVKKPSDTEGKAVAITTAPKNTTQKSKNVVAKKTPESRPLGAAYFVRLKKPNGTTED
                     ALLGSRIFSTGDRFQLGLKVNRPSYVYIFNEDSDGRITMLHPRPGGSAAVSAMGTVFL
                     PTQGSFEFQGEPGTEKLLVLMSKEEMLQPDAALYKAQPDIVAGGSPSACKVMVADAGN
                     YASKAIVYSQETNTASTNCKTTAAPLYASKAIVFADDPQPAAGQQLASYVVKPETASK
                     SEPLLLKIQLAHH"
     gene            4005..7010
                     /locus_tag="PL000_18550"
     CDS             4005..7010
                     /locus_tag="PL000_18550"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HNC90939.1"
                     /translation="MSNNHLIVGLGGTGGKVIRALRKIVKTHTDANGNSPSEANFEYA
                     YLDTSDGLLKEIDQWMVLGQDCSLARAQTGIYAASGVRPILQDPDSYPGMRGWIEPRR
                     VFDFIDSSTAGAAQKRKLGRLVFAQNARTITDLLNDRLQALASSTKNAEATIHVICGL
                     AGGTGSGFIVDTIAQLRRLCPDEASYRIMVYALLPDENSPWVKSVSGFGTYYANGYAA
                     LSELNAMACRNYRPINVLDGSRIEAAKFFNGCYLINNINENRVRFDVQESVPHIIAEF
                     LYQKTLNQRWETLARAENGENDTNFIEMDDGVGARAKVFMSFGIKRVVVPEQEIKEYL
                     AYGFAEQATRQLMFNNFRQGEGFADEPEYKDWGAEVRKPDVQERLQISDAHLTLDRGI
                     LEDDVKAEWKPTDEYWPQVAATLEAQIKSDKSIKESEWIRTLRTRLSDVFDSTFRRLG
                     GVRKFYEVKSQAQQEMARHIVRRIERDFFGRWSSGELSLLQLRQYTDAVLDNLTQRKK
                     SFEDKIQTEANVMQKLEEDLQRLTLDFNSVGTFGRVFTDKRDTRFSEAREKLQLLFSK
                     NTKRQGYMFAPRLIPLILDELTVMRGKIDQLHVQLTDATETIRTEQNNRLRTDKEEVC
                     QTRLFDIDAVRDLLRRMTLDERGQKERTQRVRKAILALAGVEVDSFEKLAARVTLNSL
                     ITMLSQQAAAITDAVHTEMAQASPVMHVNIVERLSRQYAGNSQSLNKFVVDLYREAGN
                     MVLFNKAEIERRVGNGVGAAGLQQALCVMLPECEEQREFRQEMSELFQRQKDAGISNL
                     SVLPGTLTNEVVVIKVSSLMPARFIDTLGFLKRHYDGLCKIPDEATLLHGEGDGSKLP
                     PLFAPTGVDMEKRRKRKPYLLLAKFLNFIKQRENPKTGEKEWILQSKDNDGFMLITAL
                     NGGGNWIKVWDADQSESLQNDVEKTVKSELAANYIHRERKQELLQKFVSAVNERLEQA
                     DGNADDPQYCELRAMTPTFKSIIGLNDDA"
     gene            7040..7807
                     /locus_tag="PL000_18555"
     CDS             7040..7807
                     /locus_tag="PL000_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90940.1"
                     /translation="MYKYICKSVGDCSIADRGEPITSETMLNDPKCEVCAGPLELIEE
                     SGASASSQNNGTKRGVIIAGAVAVLAIAGIGGWYVSHSKTDHQESLQTVSSQESTVQA
                     TSQEAVASTTEPMTQTVSGGIAPSEAETSTARRAADTSLGNSEFSEAEKKAARAAALE
                     MIKTAVAKMGQGDLVGAEKELMEAKARDASEPLVYYNLSIVRLRQKQPEEALKQLEAA
                     FMAGFSHFDAMDQDHDLDPLRNNPKFKELMLTYRKGS"
     gene            7897..8538
                     /locus_tag="PL000_18560"
     CDS             7897..8538
                     /locus_tag="PL000_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90941.1"
                     /translation="MAEEFRVESVSLPIGPELHITQNQLWLGTSTAPMKLRGIIADGK
                     DVTLKTELGLNIIFSFENKDRMYFSIPFVGERIYYKRDTMYAAVSASATPREAKSKQS
                     PMAVNVQTSPPLTPTAMPLSSVSPAPVTSNPTAAVITQDMNTQIHVSSASETQYELAL
                     QAMRDGDNDAALRSLSTALASGFSDWQRIDEEPLFVALASDVRFQVLRSRWKK"
BASE COUNT         2435 a         1984 c         2232 g         2223 t
ORIGIN      
        1 tgggatgaaa ccttcttgct ctctatctta ggattttaag tgcgtttgcc ctaaaatagt
       61 ctcctgtttt acttgaccat tacaaaaatt ggtggtatgg agttttatga tgttcacttt
      121 agttatatcg acttggagga tggtcaggaa catttcgtta atgtacccga acaaacgggt
      181 ggtggcctga ttcatgaagg ccgtcccaaa cctggcatcg tacacactat cgccatgagc
      241 ggcaatgaaa tgcccaccat ctatcgtatc gaaatacaaa cattggcagg cactggcaaa
      301 ttgttggtat ctggcccact atcacgtgag cctgtacgca ttgcctttga ttactttaaa
      361 gcacatgcaa gtcgagtcag tggcatgatc cgcccaagtg agcatgactt ccacttacat
      421 cttgttgatt tacaaggcgg cggagaacca gatgtatcta cattggcaag cctcattgct
      481 ttgagttctg gagctatagg tcgcccagta cagagccaat tagtcataat gggcgatatg
      541 agtctaggag gtgtcattac tcctgcaaga aacctagcag aatccttgca agttgctttt
      601 gatgcaggag ccaaaagaat acttttgcca atgtcgagtg tgggagatat tccaaccgta
      661 cctggggaac tgtttgcaaa gttccagacc agcttttatt cggatcctgt ggatgcagtg
      721 tttaaggcaa ttggtgtaga gtaatttggc ttgcttttta gggggttaag cgaaaatatt
      781 cgaaaatttt gattgtgctc tgctatcttt ttttggtaaa aataggagga ttactaatat
      841 tgatatgcta gatgctattt tagcatccat atgtcagaat ggaatgagat taagcgctcc
      901 cattcgaaaa ctaattttcc tcatctacaa ctgggaaatc ggttttttag taaaaaacct
      961 tgtcgggaag cagtgggcaa tatctataaa gtaaacctga agaaagagag aaaaatgaaa
     1021 aacatgacga aagtgtggat catggcgtca atggtgagtg tgaacgcttg gtcaggggag
     1081 tcagtgacat ctgatctaga caaatgcatc actcagaaga tgatctcctc caccgctaaa
     1141 ggtgctgtga ctggcgcact gatgggattt cttgctgcta aagcatctgg gaaagataaa
     1201 gaacatgaac ggaaggcggc actcatcggc gcggcggcag gtggtgtctt tggctatgtc
     1261 aaggcgtgga acaaggcagt cgatgactgc aagaaggagc acccagactg ggtggtcgag
     1321 tcggacatca agcgcagtcc caattacgat gctgtggtgg ctgagttcaa ttacaagcct
     1381 ccgcaaaaga atagcatcgc cattgtccgt cccttgcaga tacccgagcc agttaagcca
     1441 ggcgatacgg ccaacatcca agccaagttt gtgatattga caccagacgg cggtgaggcc
     1501 aaagtcaaga tcgatcgcaa gttctttgtt gtcgcagatg gccaagaaga accactgtct
     1561 ttccctggca gggacagcga agaccgtgtt gtcgaaaacg gtgaacaggt ggacgagttc
     1621 aatttgccaa tcggtaagga tgtaccagtt ggtacgaaag tccgtgtcca atacgctatc
     1681 tcgctgaacg gtgcatcgct tgtttcgcaa agcggcatca tcgaagtgaa atgacgatgc
     1741 gtaccgcaat aatgagaggg gtgacgctcg cattggcgat actgctggct ggctgtacgg
     1801 gttatcaatc catgccgggc tatcagcgcg tctatcaggg ttatcaggtt cctcagaact
     1861 tagctgatgc agtacaggct cgtctgcgca ccgaaggcat cagccaagct atcattggcc
     1921 gcgattccgt ggggcgttta aaactcaacg gtgtctatcg tgatgaagat gaggttgagc
     1981 gcgcattcat cattgtccag tccatcgtag gtattcagtc cacttcgccg ttctatccag
     2041 agaatgttca ggaaaagcgt tgggagcgcg aagctggggc tgctttatct gactacttca
     2101 agcgtaaaaa gcaagctcag agtaatgtac ctggaaaaaa gagagcgttg gtcattggta
     2161 ttaatcggtt ccagagttat ccacaaatca cggatattca aggtgaagat gatgctaggt
     2221 tgatgggcat agtcttagcc aactatggtt atgacgtgag gtctctactc ggagagcgcg
     2281 caactaaggc taagattgag tcggaaattg ccaatcttga taaagtgctg gagccaaatg
     2341 acaccctgtt catttatata tcgagtcatg gcactcctcc tgtgccgacg tcaggcggcg
     2401 gtgacgagcg caagatgtca attgtcgcct atgattcagg accgttgaac tccagtatgg
     2461 atcgcactgg tggtgcatta actatccaac gcacttctgt aagtgacaag ctggttcaaa
     2521 ggctagcaca gagaacgtct gcaatgacgc gtgtcattat tgacacctgc tatagtggag
     2581 agatactcag agatatgtct aacgtaggat ccgcctatca agacaagatg aatggcggtg
     2641 gttttgaaaa ggcatctgtt tctgaatcat cgtggacggg ggcttcttac accagtaagg
     2701 caattgcatt tgtggatgac ggaaatagca gcgtcgggca ggctagtaaa acatctgagc
     2761 aagtcacacg ccgcaattac agttttatta cagcaacaag cccaggtgaa ttatcttggg
     2821 gtccgccttc cactgtcgga acttttccag acccaaccag ccagagtgaa ctactcagag
     2881 gctccttctt cacacaatcg ttcatcgctt atctgaaaca attctcaggt gatgttccac
     2941 gtgcctttga ggaagctcga attttcacca gtaagatggt tcagcagggg ccaaagcagt
     3001 cacagaaccc acgccgcttc acgaccattc cagctgacat tgacaatcta tccaaacatt
     3061 aaattgaagg aatatcgcta tgaatcattg cagcctaaat aaagccatcc tcatagcatt
     3121 cgcagtaatg tctgtgggcg ttcacgccga agagcctacc tatagcgcaa aggctctctt
     3181 ctttggcgaa gatggtgaca tgaagacggt agcaactgtc aagaagcctt ccgatacgga
     3241 gggtaaagcg gttgccatta ctaccgcacc aaagaataca acacagaaat ccaagaatgt
     3301 agtcgccaag aaaactcctg agagtcgccc tcttggcgca gcctactttg tacgactcaa
     3361 gaagcccaat ggtacaacgg aagacgctct tctaggctct aggatatttt caacaggcga
     3421 tcggtttcaa ttagggctga aagtcaatag acctagctat gtttatatct ttaacgagga
     3481 ttcagacggt cgcatcacga tgttacatcc ccgtccaggt ggctcagcag cagtgagtgc
     3541 aatggggacg gtttttctgc caacccaagg ttcatttgag tttcagggag agcctggcac
     3601 agagaaattg ttggtgttga tgtccaagga agaaatgctg caacccgatg cggcactcta
     3661 caaagcacaa ccagatattg tagctggtgg ttcacctagt gcgtgcaaag ttatggtggc
     3721 agatgctggt aactatgcaa gcaaagccat cgtttattcg caagagacaa acactgcgag
     3781 caccaactgc aaaaccactg ctgcgccgct ttatgcctcc aaagctatcg tctttgccga
     3841 cgatccacag cccgctgcgg gccagcaatt agctagttac gtggtgaagc ccgaaacggc
     3901 atcgaagtct gagcctttat tacttaagat tcagttggct catcactaaa acaaaaaatc
     3961 cgcgcatatg tacatgctgc ggcgaatcaa ttattgagat aattatgtca aataaccacc
     4021 ttattgtcgg tcttggcggc actggcggaa aagtgatccg tgctctgcgt aagattgtta
     4081 agacccacac agatgcgaat ggcaacagtc cttcagaagc aaacttcgaa tacgcatatc
     4141 tagacacatc agatggtctt ctcaaggaga ttgatcagtg gatggtactt gggcaggact
     4201 gctcgctagc acgagcccag acaggcattt atgccgctag tggtgtgcgg cctattctac
     4261 aggacccaga ttcatatcca ggcatgcgtg gatggattga gcctcgacgc gtttttgatt
     4321 tcatcgattc aagcacagca ggagctgctc aaaaacgcaa attgggtcgc cttgtcttcg
     4381 cccagaacgc gcgtacaatc actgatttgc ttaatgatag acttcaagca ttagcaagca
     4441 gcaccaagaa tgcagaggca actattcacg taatttgtgg cttggcgggt ggcacaggta
     4501 gcggctttat cgtcgataca attgctcaat tgcgcaggtt gtgtccagac gaagccagtt
     4561 accgcatcat ggtctatgct ttgttaccag atgaaaactc accttgggtg aaatctgtct
     4621 caggatttgg aacgtactat gctaatggct acgctgcgct ctcagagttg aacgcaatgg
     4681 cgtgtcgaaa ctatcgcccc atcaatgtgc tggatggctc tcggatcgaa gcagcgaaat
     4741 ttttcaacgg ttgctatctc atcaataata tcaatgaaaa ccgcgtacgg tttgatgttc
     4801 aggagagtgt tcctcatatc atcgccgaat ttctttacca gaagacgctg aaccagcgtt
     4861 gggagacact tgcgcgtgct gagaatggtg agaacgatac caattttatt gagatggatg
     4921 atggtgtagg tgcccgcgcc aaggtgttca tgagctttgg tataaaacgt gtggtggtac
     4981 ctgagcagga gatcaaggaa tatctggcgt atggttttgc cgagcaggca acgcgccaat
     5041 tgatgttcaa taactttcgg cagggcgaag gcttcgctga cgaacccgaa tacaaagact
     5101 ggggtgcaga ggttcgcaaa ccagatgtgc aagagcgtct tcagattagt gatgcacacc
     5161 tgaccctaga ccgtggcatt ctagaagatg atgtcaaggc ggagtggaag ccaactgacg
     5221 agtactggcc gcaagtcgca gccacacttg aggcacaaat caagtcggat aagagcatca
     5281 aggagtcgga gtggattcgt accttgcgta cccgtctatc ggatgttttt gacagtacgt
     5341 ttcggcgatt gggtggcgtt cgtaagtttt atgaagttaa gtcacaagca caacaagaaa
     5401 tggcacgcca tatcgtgcga cgcattgagc gtgatttttt tggtcgatgg agttctggag
     5461 aactgtcatt gctccaattg cgccagtaca cggatgcggt gctggacaat ctaactcagc
     5521 gcaagaagtc gtttgaggat aagatccaga ctgaggcgaa cgtgatgcag aaactggaag
     5581 aagacctcca gcgactgact ttggatttca acagtgtggg tacctttggt cgagttttca
     5641 ctgataaacg agatactcgc ttttctgagg cacgagaaaa gttacaattg ttattttcca
     5701 agaataccaa acggcaaggc tacatgtttg ccccacgcct gattcccctt attcttgatg
     5761 aactgacggt gatgcgtggg aaaatagacc aactgcatgt ccaactcact gatgccactg
     5821 aaacgattcg tacagagcag aataatcgac tcagaactga caaggaagaa gtttgccaaa
     5881 ctcgactctt tgatatcgat gccgtgcgcg acttgttgcg ccgcatgacg cttgacgaac
     5941 gggggcagaa agaacgtacc cagcgtgtcc gcaaggccat cttagccttg gcgggtgtcg
     6001 aagtagacag ctttgaaaaa ctggcagcgc gagtcacgct taatagccta atcacgatgc
     6061 tttctcagca ggctgccgca attactgatg cagttcatac ggaaatggca caagcgtcac
     6121 ctgtcatgca tgtgaatatt gttgagcgtt tatcacgtca atatgcgggt aactctcaat
     6181 cactaaacaa gttcgtggtg gatttgtatc gtgaagcggg aaacatggtg ctattcaata
     6241 aggctgaaat agagcgacgt gtgggcaatg gcgtaggtgc agcaggactg cagcaagctc
     6301 tctgcgtcat gctaccagag tgtgaagagc agcgcgagtt ccgccaagaa atgagcgagt
     6361 tatttcaacg ccagaaagat gcaggcattt cgaatctttc tgtactccca ggtacattga
     6421 ctaacgaagt ggtggtgatt aaggtctctt ctctcatgcc tgcacgtttt attgatacgc
     6481 ttggattcct caagcggcat tacgacggct tgtgcaagat tcccgatgag gccactttgc
     6541 tgcatgggga aggtgatggc agcaagctgc caccgttgtt tgctcccact ggtgttgata
     6601 tggagaagcg tcgtaaacgc aaaccctact tgctgttggc caaatttctc aattttatca
     6661 aacaacgtga gaatccgaag actggtgaga aagaatggat tcttcaatct aaggataatg
     6721 atggattcat gcttattacc gcgcttaatg gcggagggaa ctggattaag gtctgggacg
     6781 ctgatcaatc cgaatcattg cagaatgatg tcgaaaaaac agtgaagtcg gaattggctg
     6841 ccaactacat tcacagagaa cgcaagcaag aacttctcca aaaatttgtg agtgccgtca
     6901 atgaacgctt agagcaagct gacggcaacg cggatgatcc acaatattgt gagttgcgag
     6961 ccatgactcc aacatttaag agtatcatcg gcttaaatga tgatgcctaa aattaaaaca
     7021 catcaacacg agaatgaaga tgtataaata tatatgtaag tcagttggtg attgctcaat
     7081 cgctgaccgt ggcgagccta tcactagtga aacaatgctt aacgatccca aatgtgaggt
     7141 ctgtgctggc ccgttggagt tgatagaaga gtcaggtgcg agtgccagct cccaaaataa
     7201 tggaaccaaa cgtggtgtga ttatcgctgg agcagttgcg gtattagcta tagctggtat
     7261 tggtggttgg tatgtatcgc actccaaaac agaccatcag gagtcgttac aaacagtatc
     7321 ctcacaagaa tcgacagtac aggctacctc tcaggaggca gtagcatcta ccactgagcc
     7381 gatgacacag actgttagcg gaggcattgc cccgagtgaa gccgaaactt cgactgcacg
     7441 tcgtgcagcc gatacaagct taggtaattc tgagttttcc gaggcagaga agaaagcagc
     7501 tcgtgctgcg gcacttgaga tgatcaagac tgcagtggcc aaaatgggac agggtgatct
     7561 cgttggtgcc gagaaagagc tgatggaggc caaggcacgt gatgccagcg agcctctcgt
     7621 ctactataat ctctctattg tacgcctacg tcaaaagcag ccagaagaag ctctcaagca
     7681 attggaagct gcttttatgg ctgggttttc acatttcgat gctatggatc aggatcatga
     7741 tctagatccg ttacgaaaca accccaaatt caaagagctg atgctaactt atcgtaaggg
     7801 ttcatgatgc gttggagcct gtgggtatta tcactcacta tactggtaac ggggtgcgag
     7861 aagcatcccg accctaagaa tattgaaggc cactgggtgg ccgaagaatt ccgtgtcgag
     7921 agtgtcagtt tacctattgg ccctgaactt cacatcacac agaaccaact ttggctaggt
     7981 actagcactg caccaatgaa actaagaggt atcatcgctg atggtaaaga tgttacgctc
     8041 aagacagagc ttggcctaaa catcattttc tcttttgaga acaaagatcg aatgtatttc
     8101 tctattccgt tcgttggtga acgcatctat tataagagag acacgatgta tgctgctgtg
     8161 tctgcaagtg ctacacctcg cgaggccaag tcaaaacagt ctccgatggc tgtgaatgtg
     8221 caaacatcgc cgccgctcac tccaacagca atgcctttat catccgtgtc tcctgcgcca
     8281 gtcacctcta atcccacagc tgccgtcatt acacaagaca tgaatactca gattcatgta
     8341 tcctctgcat cggaaacaca gtacgaactt gccctgcagg ccatgcgaga tggtgacaat
     8401 gatgctgcat taaggagcct atcaacagct ttggctagtg gattttcgga ttggcaacgt
     8461 attgatgagg agccgttgtt tgtagcatta gccagtgatg tccgatttca agtcctgcgt
     8521 tcgcgttgga agaagtgagt ataagcccca accgccgaaa atttcgcctt aggggttgat
     8581 gataaatact gataggggct tagtgtttgg ataatttcgc tttttgtgac tatcggtaaa
     8641 tttccttgca aaacagttgg tggaattttg ggcgcaatgg aacggcgaac cgacttaaca
     8701 tacaccccct aaaattcacc accgatactc cgtaccctgc tgccatgcct ccgcctcaat
     8761 ataacaatga cgcatctcag cctcatggct catatcacgc aataaatcag ccattaatcc
     8821 atctaaatct tcacgggttt tataattgat gcgtaagcta taatcaccag acga
//
LOCUS       DAOQLC010000151         8764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3161_length_8764_cov_23.915145, whole genome shotgun sequence.
ACCESSION   DAOQLC010000151 DAOQLC010000000
VERSION     DAOQLC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3161_length_8764_cov_23.915145"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4148)
                     /locus_tag="PL000_18565"
     CDS             complement(<1..4148)
                     /locus_tag="PL000_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90942.1"
                     /translation="MKDNWFRKASSVFLVVFLMMAVLPVQNAFAAVTRPTDWTSVGSS
                     VGNGTTLSTTGTYAAAAGTERYTLLAVTVVNTGATVTSTVSGTWGGVAFNSNTPTRFI
                     GQVGNGTSRRVIYLFGFNETDIAARANDTITISSSLNSATTYMHSYMSTYAGVNQSTP
                     TTVSSVDNGGGPTTTAQYTAALNTVNGSYGASILLVGGTTGGGVTNTDAAATPNTGYA
                     MNVNNVQVGAASGTYATLGNKAFTGTGTTQPTFTFANNRNVLIGVVLAPSLPNTTTAG
                     TAAATAASSTSINVSMPYTDDGNANNTYTVDYKLSSAVGWTNWVTNAAHTASPYTTTI
                     TGLSSATNYDVRVTYNDADGVTGTNPQTISNVTTLTNIFDTSIRFTGTGNPLTQSYTI
                     GSGGDLLVVSIVAGGATSRTGGAPTYNGVALTQADATRTATETVTEMWYLVNPPNGAA
                     YNISIPNNGGLTLYATASSYFAQSGYTYALDVANGNTNGNSANPSVSVTTTTNGDVIV
                     GAMGNGNNAAPTGRSGTMLYENDNGLFSDNSQYFYQGTAGAYATSWTITADDWGVVVA
                     AFKPVPTTTTVGLATATAASSTTINVSMPYTVDNNANNTYTVDYKLSSAVGWTNWVTN
                     AAHTASPYTTTITGLLPASDYDVRVTYNDADGVTGVNPQTISNIFTSLADTSVRFTGA
                     GNPLTQSYTIGSGGDLLVLSIVAQGATARTGGAPTYNGVALTQADSTRIATETVTEMW
                     YLVNPPNGAAYNISIPNNGGLNLYATASSYFAPSGYTYAFDVANGNAVGGVGSANPSV
                     SVTTTANGVIVGAMGNGNNAAPTGRSGTMLYENDNGFFSDNSQYFYKAPAGAYATSWT
                     IGADDWGVVVAAFKHTILPNSTTVGSATAVAASSTTINVSMPYTDDGNLNNTYTVDYK
                     LSSAVGWTNWVTNAAHIASPYTTTITGLLPASDYDVRVTYNDADGVTGTNPQTISNIT
                     LPATPISGSWTNVYSGTGTTVVNAVSATSTSTCTAGAGSNRVVLVAFSASAAAAANSN
                     FSMTAPTATFGGVSVTEITSTKASADLRPHAWIGYIKEANIPAGAQNISIAFTKGGTN
                     NSAVANISIFCATFTNVDQTSVYSHAVAGPNAAFQPASPTISMNVGVNDQAILFTPTS
                     ATATVNNYTPASYVSASFLGTVGYSVDIAHRTIPASAVAGENATVTWSANGRSSILGV
                     TLKRAPNKLDQVITVGTAAPANAVFGTTFPVAATSDSGLNVAITTSGGCSGSGTSNAV
                     ITMTSGTTACVVHYNQAGDTAYNAAPEVTSSTTAQKATPVITFNAAPTPTYLGGNFVV
                     SANTTNTDSNALTYSYVSGPCAFVSGGTFSSTGAGTCVAQANGAATANFNAASQTQNV
                     SIAKA"
     assembly_gap    4175..4274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4275..8155)
                     /locus_tag="PL000_18570"
     CDS             complement(<4275..8155)
                     /locus_tag="PL000_18570"
                     /inference="COORDINATES: protein motif:HMM:NF027952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90943.1"
                     /translation="MHRQFIAKIAAALMIVMTALGAMPVTPAFAADCANTSTTQYRSA
                     NTGNWNAPATWECSDDGINWQVPTTNTPTNANSFITIRNTHNVTVSANVTADQLTIDA
                     GGQVTVNTGITWTLNNGTGATDVTINGTIENSGTMADIGNAVLVFNSGSIYRHAQNGG
                     VIPSSVDFAPVTWNTASTIEITGITSTAPSGLAQSFGNFTWNSTSQGATTISLGGNLT
                     TINGNFTVNSTGTGATRINGGATDITVNIGGNYTQTAGTLIFSSGTGDVTFNVGGNFS
                     VSGGVFNMSNNDAAVGTVNLTGNFTHTSGTIREIALNTGTFNFLTGTHSFTSGGTVTG
                     DINFVVNNGATLNMGTSTMSGNIFTLSNGGTLGIGSTNGITTAPTASGNIQTTTRTYN
                     TGANYTYNGTASQAAGNGLPTTVNNLTIDNTGGAGNNTVSLSTSHTVNGVLTFTNGNI
                     STGANSIILPVAATVSGAGAGKYVNGNVQRAFTAGSPTFTFPIGDGSNYAPVDLTFTG
                     ITTGGDVTASTTASDCAGSSGIDTTKSANHCWTLTNSGTVFTSYSPTFNFAGVGTDAD
                     AGANTANFIVRRFSGGTWSATTNGTRTATSTQATGVTAMSDFAVGESSLANTTTTVNC
                     ATNPVTVFSGTVCTATVTRASGANTPSGTITWGTDGAGAFTAANTCVLAATATPGVSD
                     CSVTYTPSAVGTGSHTVTASYGGDSNFNSSNGNQVVTVNKATPTITFGAAPTPTYLGG
                     NFTVSANTTNTDSSTLTYSYVSGPCAFVSGADFSSSGAGTCVVQANGAATANFNAASQ
                     TQNIAIAKATPTITFGAAPTPTYLGGNFTVSANTTNTDSSTITYSYVSGPCAFVSGAT
                     FSSSGAGNCMVQADGAATTNFNAASQTQSITIAKATPTINFGAAPTPTFPGGNFTVSA
                     NTTNTDSSTLTYSYVSGPCTFVSGADFSPTGTGTCVVQANGAATTNFNAASQTQNVLI
                     SAPGATTPTITFDPAPNPTYLGGNFTVNATTTNTDSSTLTYSYVSGPCAFVSGATFSS
                     SGAGTCVVRADGAATANFTAASAIQNVTIAKANPTLSISNPTVTFTGAPQTANVFGSV
                     AGTVSNVLYNGSATAPTNLGTYAVTADFAPTDTTNFNSLVGASAGNFTIQPAGSGVDY
                     PTFLDVPTDYWAWQYIEAIYAAGITSGCGNGNYCPTNNVTRAQMAIFLLRGIHGSYYT
                     PPAATGTVFADVPADYWAASWVEQLALEGITGGCGNGNYCPDAPITRAEMSIFLLRAK
                     YGSAYVPPTATGTLFIDVPADYWAVDWVEQL"
     gene            complement(8229..8459)
                     /locus_tag="PL000_18575"
     CDS             complement(8229..8459)
                     /locus_tag="PL000_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90944.1"
                     /translation="MEKKSRIQELAERVNMTYDEFIGEMRKRGCSEPTASKIWRGEYE
                     NFVDFKDNDVNLSNLRKAAAVLKVGTGILLPK"
BASE COUNT         1905 a         2014 c         2478 g         2267 t
ORIGIN      
        1 gccttggcga tcgaaacatt ctgtgtttgc gatgcagcat tgaaattagc cgtcgctgct
       61 ccattggctt gagcaacaca ggtccctgcg ccagtggaac tgaatgttcc accgctgaca
      121 aatgcacatg ggccgcttac atagctgtag gtcaacgcat tactgtctgt gttggtggta
      181 tttgcgctga caacgaagtt gcctcccaaa taagtggggg tgggcgctgc attgaatgtg
      241 atgactgggg tcgccttttg cgccgtggtg gagctggtca cttcaggagc ggcgttataa
      301 gctgtatctc cagcctggtt gtagtgaacc acacaggctg tcgtgccgct ggtcattgtg
      361 atcacagcgt tggatgtacc gctgccggaa catccaccac tggtggtgat cgccacattc
      421 aggcctgaat cggatgttgc agccactggg aatgtagtgc cgaagaccgc atttgcaggg
      481 gccgctgtac caacggtaat aacctgatcc agcttattgg gcgcacgctt taaagtgacg
      541 ccaaggatgg aagatctgcc atttgcactc caggttacag ttgcattctc accagccacc
      601 gcggaggctg ggatcgtcct gtgcgcaata tctacgctat agccgacagt gcccaagaac
      661 gatgcggata cataagaggc aggagtatag ttattaaccg tcgcggttgc cgaagtgggt
      721 gtgaaaagga tggcctgatc attcacacca acattcatgg aaatggtcgg agaagcgggt
      781 tgaaatgcag cattgggtcc tgccaccgca tgcgaataga ccgaggtttg atccacatta
      841 gtaaatgtgg cgcagaagat gctgatgttg gcaactgcgc tattattggt gccgcccttg
      901 gtaaaggcaa tgctgatatt ttgggcgcca gccggaatat tcgcttcttt gatgtaaccg
      961 atccatgcgt gcggacgaag gtctgcactt gctttcgtac ttgtgatttc agtgaccgat
     1021 actccaccaa atgtggcagt gggggccgtc attgaaaaat tggaattggc tgcggcagct
     1081 gctgaagccg aaaaagcgac caaaaccacc cggttgctgc ctgcccctgc cgtacacgtg
     1141 cttgtgctgg tagcggatac ggcgttaaca accgtcgttc ctgtgccgct atatacattt
     1201 gtccaactgc ctgagattgg agtggcgggc agtgtaatat tgctgatggt ttgcggattt
     1261 gttccagtaa caccatcggc atcgttatag gtcacacgta catcatagtc gcttgcaggc
     1321 agcagacccg tgatggtggt cgtgtatgga gatgcgatgt gagctgcgtt tgtcacccaa
     1381 ttcgtccaac caacagcgga agagagcttg tagtcaaccg tataggtgtt gtttaagttg
     1441 ccgtcatctg tatacggcat ggaaacattg atcgtggtcg aacttgctgc aactgctgtg
     1501 gcagaaccaa ccgtggtgct gttgggcaat attgtatgtt tgaaggccgc aacaacgacc
     1561 ccccaatcat cggcgccgat ggtccagctg gtggcataag ccccggcagg cgctttataa
     1621 aaatactggc tgttgtcaga gaaaaagccg ttgtcgtttt cgtacagcat ggtgccgctg
     1681 cgaccagtgg gagctgcatt gttcccatta cccatcgcac cgacgattac tccgtttgca
     1741 gtcgtggtga cagaaacgga tggattggct gaaccaaccc cgcccacggc gtttccgttg
     1801 gccacatcaa aagcataggt gtagccgctc ggtgcaaaat aggaagaggc agtcgcatac
     1861 agattgaggc cgccgttatt ggggatgctg atgttgtagg cagcaccgtt gggcgggttg
     1921 acgagatacc acatttcggt gacggtttca gtggcgattc tggtggagtc cgcttgggtg
     1981 agggcaacac cattgtaggt gggtgcgccg ccggttctgg ctgtagcacc ttgtgccaca
     2041 atggatagga caagcaagtc cccgcctgac ccgattgtat aactttgggt caacggattc
     2101 cccgctcccg taaagcggac acttgtatca gcgagagatg taaaaatatt gctgatggtt
     2161 tgcggattta cccctgtgac accatcggca tcgttatagg tcacacgtac atcatagtcg
     2221 cttgcaggca gcaaccctgt gatagtggtt gtgtaggggg atgcagtatg ggcggcattt
     2281 gtcacccaat ttgtccaccc aacagcggaa gagagtttgt agtcaaccgt ataggtgttg
     2341 tttgcattgt tatcaactgt atacggcatg gaaacgttga tcgtagttga acttgccgca
     2401 gtagctgttg ctaagccaac agtagttgtc gttggaactg gtttgaaagc cgcaacaacg
     2461 accccccaat cgtcagcagt gatcgtccaa ctggtggcat acgcaccggc ggtcccttgg
     2521 taaaaatact ggctgttgtc agagaaaagg ccattgtcgt tttcgtacag catggtgcca
     2581 ctgcgaccag tgggagctgc attgttccca ttgcccatcg caccaacgat cacatcccca
     2641 tttgtggttg tggtgacaga gacagaggga ttggcagagt tgccgttggt gttcccattt
     2701 gcgacatcaa gagcataagt gtagccgctc tgtgcaaaat aggaagaggc agtcgcgtat
     2761 agggtaagac cgccattgtt ggggatgctg atgttgtagg cggcaccgtt gggcgggttc
     2821 acgagatacc acatttcggt gacggtctca gtagcggttc tggtggcgtc tgcctgggta
     2881 agggcaacac cattgtaggt gggtgcgcca ccggttctgg aagttgcgcc tccggccaca
     2941 atggatacga caagcaagtc accgcctgac ccgattgtat agctctgggt caacggattc
     3001 cccgttcccg taaagcggat gcttgtatca aatatgtttg ttagtgttgt gacgttgctg
     3061 atggtttgcg gatttgttcc agtaacacca tcggcatcgt tataggtcac gcgtacatca
     3121 tagtttgttg cgctactcaa gcctgtaatg gtggttgtgt agggagatgc agtatgagct
     3181 gcatttgtca cccagtttgt ccacccaaca gcggaagaga gtttgtagtc aaccgtatag
     3241 gtgttgttcg catttccatc atcggtatat ggcatcgaga cgttaatgga tgtggaactt
     3301 gcggctgttg ccgctgctgt acccgctgtg gtggtgttgg gaagggacgg ggcaaggacc
     3361 acaccgatca agacatttct gttattcgca aatgtgaagg tcggctgggt ggttcctgta
     3421 ccggtaaatg ccttgttgcc cagggttgca taggtgccac ttgccgcccc tacttgaacg
     3481 ttgttaacat tcattgcata gccagtattt ggggtggcag ctgcatctgt gttggtgacc
     3541 ccgccgcctg tggttccacc tacgagcaga atcgaagctc catagcttcc attgacggtg
     3601 ttcaatgccg cagtatattg cgcagtagtg gtagggccgc cgccattatc aacggaacta
     3661 actgtagtag gagtggattg gtttaccccc gcataggttg acatataact gtgcatatat
     3721 gtcgtagcgg aattcaaaga ggaactgatc gtgattgtgt cattggcgcg tgcggcgata
     3781 tccgtttcat tgaaaccaaa caaatagatc acacggcgac tggtcccatt tccgacctga
     3841 ccaataaaac gagttggagt attgctatta aaggccactc cgccccaggt tccactgacc
     3901 gtactggtta ctgtcgcgcc cgtattgaca actgttacag caagcagggt atagcgttct
     3961 gtcccagccg ccgcagcata ggtgccggtg gtgctcagtg tagtgccatt tcccactgaa
     4021 cttccaactg aagtccagtc tgtggggcgg gtcacagccg caaacgcgtt ctgtactggt
     4081 agaaccgcca tcatcaaaaa gacaaccaaa aaaacggatg aagccttgcg gaaccaatta
     4141 tctttcatat ctatctcctt gcttttgttt ggatnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnntataac tgctcaaccc aatccactgc ccaataatcg gcggggacat
     4321 caataaagag ggtgcctgtt gcggttggcg gcacataagc acttccatat ttggcacgca
     4381 acaggaagat ggacatttct gcacgagtga tcggcgcatc cggacagtaa ttgccattgc
     4441 cacatccgcc ggtgatgccc tcaagtgcaa gttgctcaac ccagcttgcc gcccaatagt
     4501 cggcagggac atcggcaaat acagtccctg tggcggcagg tggagtgtag taggaaccat
     4561 gaattccgcg aagcaggaaa atggccattt gtgcgcgtgt gacattattg gtcggacagt
     4621 aattaccatt tccacaacca ctggtgatgc ctgccgcgta aatggcttcg atatattgcc
     4681 aggcccaata atcagtgggt acatcgagga aggttgggta atccacaccc gaaccagcag
     4741 gctggatcgt gaaattcccg gcagaggctc caaccaggct gttaaagttg gtggtatcgg
     4801 tcggcgcaaa atcagctgtt acggcatacg tccccaaatt ggtcggtgca gtggcagagc
     4861 cgttataaag aacattactg accgtgccag cgacagaccc aaacacattt gcagtttgtg
     4921 gagcacctgt aaatgtaact gttgggtttg aaatggatag agttggattc gccttggcga
     4981 tggtgacatt ctgtattgcg gatgcagccg tgaaattggc ggtggccgca ccatcggctc
     5041 tgacgacgca ggtgcctgct cctgaagaac tgaaagttgc gccgctgaca aatgcacacg
     5101 gaccactcac ataactgtaa gttaatgtgc tgctgtcggt attggtggtt gttgcgttga
     5161 ctgtgaaatt accgcccaga taagtgggat tcggtgcagg gtcgaatgtg attgtcggtg
     5221 ttgtggcgcc aggcgcagag atgaggacat tctgtgtttg tgaggcggca ttgaaattgg
     5281 tggtcgctgc accattggct tgaaccacgc aggtgcctgt tcccgttggg ctgaaatctg
     5341 caccgctgac gaaggtacat ggaccgctca catagctgta ggtcaatgtg ctgctatcgg
     5401 tgttggtggt gttggcgctg acagtgaaat tgccgcctgg gaatgtgggt gttggtgccg
     5461 cgccgaaatt gattgtcggg gtcgctttgg caatggtgat actctgcgtt tgtgaggcgg
     5521 cattgaaatt ggtcgttgcc gctccatctg cttgaaccat gcagttgcct gcccctgaag
     5581 aactgaaagt tgcgccactg acaaaagcgc acggaccact cacataacta taagttattg
     5641 tgctgctatc ggtgttggtg gtgtttgcgc tgacagtgaa gttgccgccc aaataagtgg
     5701 gagttggggc tgcaccgaag gtgatcgttg gggttgcttt ggcaatggcg atattttgcg
     5761 tttgtgaggc ggcattgaaa ttagcggtcg ccgctccatt ggcctggact acacaggtgc
     5821 ctgctcctga agaactgaaa tctgcaccac tgacgaaagc gcacgggccg ctcacataac
     5881 tgtaggtcaa tgtgctgctg tcggtgttgg tagtgtttgc gctgacagtg aagttgccgc
     5941 ccaaataggt gggggttggg gcagcgccaa aagtgatcgt cggggtggct ttgttaactg
     6001 ttacaacttg attcccattg ctgctgttaa agttcgagtc gccgccatag gatgctgtaa
     6061 cagtatgcga gccagtgcct actgcgctgg gggtataggt gacggagcaa tcggaaaccc
     6121 caggtgtggc ggttgcagcc agaacacagg tattcgctgc cgtaaaagca cctgccccat
     6181 cggtgcccca ggtgattgtg ccactggggg tatttgctcc gcttgctcgt gtaacagtgg
     6241 cggtacagac ggttccgctg aacactgtca ccgggttggt ggcacagtta acggttgtgg
     6301 ttgtgttggc aagggaagat tctccaacag cgaaatcgct catggctgtt accccggttg
     6361 cctgtgtgct tgtggcggtg cgggtaccgt tggtcgttgc agaccacgtc ccacctgaga
     6421 accttcggac gataaagttg gcggtgtttg ccccggcatc tgcatcggtg ccgacaccag
     6481 caaagttaaa agtggggctg taactcgtaa ataccgttcc tgaattggtc agggtccagc
     6541 agtgattggc gctctttgtc gtatctatac cggaactgcc cgcacaatcg cttgcagtag
     6601 tggatgcagt gacatctccc ccggtggtga tgccggtaaa tgtaagatca actggcgcgt
     6661 agtttgatcc gtcgccgatc gggaaggtaa atgttggaga cccggcagtg aatgcccgtt
     6721 gaacattccc gtttacatat tttccagcac ctgctcctga aaccgttgct gcaaccggca
     6781 gaatgatgga gttggctccc gtggaaatat taccgttggt aaaggtgagt acgccattca
     6841 cggtgtggct ggtgcttagg gagactgtgt tatttccagc gccgccagtg ttatcaatgg
     6901 tgaggttatt aaccgtggtc ggaaggccat tgcctgcggc ctgactggct gtgccgttgt
     6961 aggtgtagtt cgctcctgtg ttataggtgc gtgtggttgt ttgaatgtta cctgaggcag
     7021 tcggcgctgt ggtaatgccg ttcgtcgagc caatgccgag tgtgccccca ttggaaagtg
     7081 tgaagatatt gcccgacatg gtactggttc ccatgttaag cgtggcacca ttgtttacga
     7141 caaagttaat gtcaccggtg accgtgcctc ctgaggtgaa ggagtgtgta ccggtcagga
     7201 agttgaacgt ccctgtgttg agagcaattt cgcggatggt tccgctggta tgcgtgaagt
     7261 tgcctgttaa attcacggtg ccgacggcgg catcattatt gctcatgtta aatacgccgc
     7321 ctgataccga aaaatttcca cctacattga aggtgacatc cccggtcccg gaactaaaaa
     7381 taagggttcc tgcagtttgg gtgtaatttc cgccgatgtt gaccgtaatg tcggtggcac
     7441 cgccgttgat acgggtggct ccagtgcctg tgctattgac cgtgaaattc ccgttaatcg
     7501 ttgttagatt gccgcccaat gagatggtcg tggctccctg actggttgaa ttccaggtaa
     7561 agtttccaaa tgattgagcc aggccgctgg gtgctgtgga tgtgatgccg gtaatttcta
     7621 tggtggaagc ggtgttccat gtgacggggg caaaatcaac cgaggatggg atcacaccgc
     7681 cattttgggc gtggcggtaa atgctgccgg agttgaaaac caatacagca ttgcctatat
     7741 cagccatggt gcccgagttt tcgatcgttc cattgatggt gacatcggtg gcgccggtgc
     7801 cattgttgag ggtccatgtg atgcccgtgt tgaccgtcac ttgcccgcct gcatcaatgg
     7861 tgagttggtc agctgtgaca tttgcagaaa cagttacatt gtgcgtgttg cgaatggtaa
     7921 tgaagctgtt tgcattggtg ggggtatttg tagtgggcac ctgccagtta atgccgtcgt
     7981 cagaacactc ccaggttgcg ggggcgttcc aattgccggt attggctgag cggtattgtg
     8041 ttgtggaagt gttggcgcaa tctgccgcaa atgccggtgt tacaggcatg gcccccagtg
     8101 cggtcattac gatcatgagt gcagctgcga tcttggctat aaactgtctg tgcatgtttg
     8161 tatcctcact ttatattagg ctgataacat cgactcacac gacttccgca acttgctgtt
     8221 tgcaggatct actttggcag taatatgcct gttcccacct tcaaaacagc cgccgctttg
     8281 cgtaaattac tgaggttgac atcgttatct ttgaaatcta caaagttttc atattcgccg
     8341 cgccagatct ttgatgcggt tggctcgctg catccccgtt tgcgcatttc tccgatgaac
     8401 tcatcgtagg tcatgttgac tctctctgcc aattcttgaa ttctgctttt cttttccatt
     8461 tttctcttcc tttttttgca tgatattgct cactttacaa tggatttgta aagtcggctt
     8521 ttgggtggat ggtggtgtct atgatggagg atgggcatcc ccacagcctg tgaaaattta
     8581 taaagttgaa atctgcctta attttgtttt gcaagctgga gcacatgtgg gtttataaat
     8641 ttatttttaa gcggaggtaa aaaaaacgtg caatgttttt gcaaggcagc ttgcatttct
     8701 gatagctaca agttcatttt acagagattt gacaaagagt caagatgaaa acagctgatt
     8761 atgc
//
LOCUS       DAOQLC010000152         8589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3260_length_8589_cov_19.608390, whole genome shotgun sequence.
ACCESSION   DAOQLC010000152 DAOQLC010000000
VERSION     DAOQLC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_8589_cov_19.608390"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1154..1492)
                     /locus_tag="PL000_18580"
     CDS             complement(1154..1492)
                     /locus_tag="PL000_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90945.1"
                     /translation="MKTTFFLLSLLLLSACQTTGTQSNLRAFSLTDVRAAKVDADAHS
                     DALASLCYGELITQLQVAEADPVGLVSTYQRARDVRRYAESDKLHTACGPLAVDAIMT
                     LRSPIVGVVR"
     gene            complement(1493..1984)
                     /locus_tag="PL000_18585"
     CDS             complement(1493..1984)
                     /locus_tag="PL000_18585"
                     /inference="COORDINATES: protein motif:HMM:NF013152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HNC90946.1"
                     /translation="MSTKQSPSKTSDRGVEFICAWEKYSPIPYTDQSGHITWGYGHAQ
                     RKGEAVPKYVSEEAALDLMKKDITVAEDAVAAQVKVHLAQHQFDALVSLCFNIGSTNL
                     AESTLLKLLNSEKYVSAANQFLVWNKVRNPRTGELEVSNGLLVRRSSERNLFLSANYD
                     ASH"
     gene            complement(2307..2687)
                     /locus_tag="PL000_18590"
     CDS             complement(2307..2687)
                     /locus_tag="PL000_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90947.1"
                     /translation="MVDPSTQSAVAVAVTGAGITVFGVALGLHPELLLAGFCGAMWSL
                     SICDPMPALRRITVSTISTFVAGYLTPALSAVVLAHNVSWLPPNLTGEIVQYPLAMMI
                     GFLSHQVIGPWLLRAAGKKAEVTK"
     gene            complement(2687..3343)
                     /locus_tag="PL000_18595"
     CDS             complement(2687..3343)
                     /locus_tag="PL000_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90948.1"
                     /translation="MSNKTSLLDLIYQSQSSKETTANNLFDAASPAMLFGRRASLCSG
                     LNWFYYGGYMLVDGVLTAIANNAAALALTASSNNYIEATRAGVVSANTTGFTPGRIPL
                     YLAVTSTSTVSDYTDYRPWVQPVAVTSKASVAVTTADYTLSAAEARCGYLTTTGALTG
                     NRNVIVPNDWQGIVFCNNTGAFTTTFKTQSGTGIVVAQGKRAVLFADGTNVVRITGDT
                     "
     gene            complement(3348..6575)
                     /locus_tag="PL000_18600"
     CDS             complement(3348..6575)
                     /locus_tag="PL000_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNC90949.1"
                     /translation="MSSAGQIAGAIVGGVIGFFAGYPFLGAQLGMLLGGSINPPKGPT
                     TYGPTLSDLTVQTSTYGAAIPRGYGAFSVVGNIFWLENNQLKATVTKKKSGGGKGGGK
                     KTTTVTTTYSATFAVGLCKGPIVGVRRIWIGPTLIYDATSSDPSTIAASNSAASGFVV
                     YTGTDTQQPDTRMQATLGVANTPAWRGLAYIVFYDLQLANYGNSLAGAQVKVEVLTLG
                     ATYSYTYNAVTTPGGYYFGRSAWNGSVFCAISGTSSGVCWTSPDGVTWTPRTMPETNS
                     WADIASNGSLFVVLVLDWHVNYCYTSPDGINWTKRYFQFPSVTAGYHTRIIWNGAKFL
                     VGTDDAGYVPFETSPDGITWSSQTSPYSGVTSCLAWNGSKFLAVHNSQFWTSPSGNAG
                     TWTLAYTDPGGGINWNSATAKGNLFCVASDDGGRPTCTSPDGVTWTKTTQSFPTNLNG
                     LATDGRVFLAGSNGRYYTSPDGVTWSYTAVTPSGEYSYPCFNGAQFLLLVPTTTKGIL
                     VTPSFISAIQPTLSQVVSGECLLSGNLNSGDIDTTLLSSNVTGYRIGQQGSIRNSLEP
                     LQTAFPFDVVQRGYKIKFIPRGNSAVMTIAAADLDAHSSKDKPGVQITTKREVDSQLP
                     RRVIVKFLDMDREYDVGSQYAERLNTSAINEKTVDLPIVFNSTDAAGKAQIILYSAWL
                     ERTDIQFNLPSTYNALEPADVVNLPTPEGVIAVRLVGISYTSDHRLECKAKVNVAAVY
                     TPNAVGVAGAVTGQTTLVPVGASTYLLLDLPRMSDSQADPCLLLAMTGVKAGWKGGSL
                     FQSTDSGSTWADIYDVGAPGSTMGIATNSIGAVEQRMKDSGSSLSVKLTQGSLSSITE
                     LAMLAGGNHFAYGVNGRWEIIAAQTCTLVSGTTYTLTNLLRGRYGTEQYMGSHVNGDS
                     VVLLDSTTLSLLSMSSSVIWLSRLYRGITFDRDISTDSNLSFSYNAINLKPLSPVYLT
                     GDRDNGFDWNLSWLRRTRANAEWMDSVDAPLGETSEAYQIDVFSDGTYTSVKRTISTT
                     SPNTIYSSSNQIADFGSNQSTLYLKIYQMSEVVGRGYPLTQSITR"
     gene            complement(6572..6982)
                     /locus_tag="PL000_18605"
     CDS             complement(6572..6982)
                     /locus_tag="PL000_18605"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNC90950.1"
                     /translation="MLTSEIVAAARQCLGTPFVHQGRVVGVGLDCAGVCCHVAGSFGL
                     DVVEVQGYGETPNKGMLEQALDMQDYLMVVEDMRPGDILLMRFFSEPQHLAILTEDNT
                     IIHAYETIGSCVEHGLDDRWLSRIVRIYRFKDAV"
     gene            complement(6982..7815)
                     /locus_tag="PL000_18610"
     CDS             complement(6982..7815)
                     /locus_tag="PL000_18610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HNC90951.1"
                     /translation="MKASVADYRFRVLCMRIVPTTGSPIYLTDYPTNLVMNSHTYLST
                     NGYQFSGYNAEAGFSPSSIDLEGIAGAAGISRAQIGSGLFDGARCYAFATTWSNPVED
                     QEPIVAGIFGQASLMDNRYTINGMSLVDALNQSVGDIYTPSCPKVFCGQEYGGCKANL
                     AANTVTGTLTAVASGSIFTDSSRLEAADTFGAGTIQFTSGPNAGFKPQEIKSFSGGVI
                     EVFEPFYYLPAVGNTYSMVRGCRKTIAACQARVGGSNIVNFGGFPWIPTGSTYATFGT
                     G"
     gene            complement(7812..8456)
                     /locus_tag="PL000_18615"
     CDS             complement(7812..8456)
                     /locus_tag="PL000_18615"
                     /inference="COORDINATES: protein motif:HMM:NF020901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HNC90952.1"
                     /translation="MASFLEERLSLNVREGASYTEDYSVEITTTASDAEYRVLRHGFP
                     KRKWTINFTLTRDNMGRLVKDLYDRVFGKFAGFRVKCLDDFTSAVDGMSSPTKDDQTL
                     QYVSSGVYQLRKVYGLSAPGLGVGRPSRTIYKPVAGTVIVAKNGTLLSSGVSVDTTTG
                     LVTISPAPSYPADTITAGFEFDLPCRFDSSFASHALSSDLRDCGSIDIVELLSP"
BASE COUNT         2237 a         2171 c         2087 g         2094 t
ORIGIN      
        1 acgatcaacg ttttatacag catcttgttg ttcatataca ccagagacct atcgcgcaat
       61 tacagcaacg cgatacgtgt tctacgtaat gtatattaca agctgttctc aaaacaaaac
      121 agcttagaag agattcgcat gcttatcaca gaagctttga tcgagagcgg tgagggtttt
      181 tagccgcgac gcagccacag acagttgaca ctgtgcgcaa aacccgacat aatcttttct
      241 gcgtggtgga tgttgtcgga tacttcattg gcgaaataaa accgacatcg cttgttcccg
      301 caacattggt ggtgattgtt gcagttactt cttcccaacg aaatcggctc caggccgata
      361 ctactctgca actacttgtt ccccaaaaac accaaagacc gcgcattgtc gcggtctttt
      421 tactggttct ccacaaatta tcggagattt catatggacc attttggaat aggtgcaggg
      481 atacgtgggt ctgtatccac gtatctgcga tgcgctcgca gtactggaag aacaacttca
      541 ttggttgaaa gcgtgaagga aggggacaga gttgtctttt caaccatgaa cgaagctacc
      601 agggttcatc gtttgtgttt gtacagaaag gtgaagatcg aatgcgtcgt tgttgatcca
      661 cgagaaccac acaggttgtt tgagaaaggt ccttctcagg gtagaacttt gtttgatcac
      721 gcctgggtag agcagtacta tctcgaagca atagacaaag cctaccaaaa actggacagt
      781 ttagagttgg agttgtctgg gtatcaagag gaattccgta gagataacaa gcgcagagaa
      841 gaagaaataa gcaggtggga accatagggg tgtttgaggt tccggccaga cacggtaagt
      901 ctcggaaata ggaacagttc gattcccttc tgggcagaaa cacaaacccg catttctgcg
      961 ggttttcttt ttgttatcag caattagctt tttggaggta ccgtcaacgc ggtgtgaaag
     1021 acacctatac tcagcaaacc tgtcttgata gcgtccacca gcggtgctgc gtcagccctg
     1081 tctgttatga caaggaacac ccacaccaag aacagcaggg ctgcgaacac gaatgttggg
     1141 atgttgtctg aattcaacga accaccccaa caatcggtga gcgcagagtc atgatggcgt
     1201 ccacggccag tggaccacat gctgtatgta gcttgtccga ctctgcgtat ctgcgcacat
     1261 cgcgtgctct ttgataggtg gacaccaatc caacagggtc cgcctctgca acttgtagtt
     1321 gagtgatcag ctcaccgtag caaagagaag caagggcatc agaatgagcg tccgcatcaa
     1381 ctttcgccgc ccgcacgtcg gttaaggaaa aggcacgcaa gttggattgc gtacctgtag
     1441 tttggcatgc agaaagcagc aagagggaaa gaagaaaaaa cgtggttttc atttaatggc
     1501 tcgcatcgta gtttgctgaa aggaagagat ttctctccga cgatctcctg acgagcagac
     1561 cgttcgacac ttcgagttcc ccagtgcgtg gattgcgcac cttgttccag acgaggaatt
     1621 ggttggccgc actcacgtat ttctcgctat tgagcaactt caatagcgta gattccgcaa
     1681 ggttggtgga accaatgttg aagcagaggg ataccaaggc gtcgaactgg tgttgtgcca
     1741 gatgaacctt cacctgggcc gcaaccgcgt cctcggccac ggttatgtct ttcttcatca
     1801 gatcgagagc agcctcttca gacacgtatt ttggcacagc ctcacccttg cgttgagcgt
     1861 gaccatatcc ccatgtgata tgaccagatt ggtccgtata tgggattggg gaatacttct
     1921 cccatgcgca gataaactca actccacgat cgcttgtctt cgacggcgat tgctttgtgc
     1981 tcatttcact cctccaactt gtccattaaa aggtatcggg gctcgcctat ctcctcgtac
     2041 acgaggacgc aatctatctc tcaccaaaag aagggcaata cccgaaacaa taatcgtctc
     2101 cggccaattc ggtttgtcac cgagagccac atatagcgca ctcccggccc cgcccaccgt
     2161 gaggcaatgg aacgctagtc gagttaacca accagaacac ggggacatgc gattgatcgc
     2221 tggctcacca aggaacatga caatgagaga cgaaacaaac acgattattt gcatgagagg
     2281 ggtgcagaat tctggagtat ctgggatcat tttgtcacct ccgctttttt accggcagcc
     2341 ctcagcaacc atggtccgat gacttggtgt gacaaaaagc caatcatcat agctaaggga
     2401 tactgaacga tttctccagt taagtttggt ggcaaccagc ttacattgtg ggctagtaca
     2461 acagccgaca gtgctggtgt gaggtatcca gctacaaaag tagaaattgt ggaaactgtg
     2521 atgcgcctaa gagctggcat cggatcacaa atcgacaacg accacattgc gccacagaaa
     2581 ccggccagca acaattctgg gtgtagccct agtgctacac caaaaacagt tatgcctgcg
     2641 cctgtgaccg caaccgcaac cgcagactgg gtggatggat caaccattag gtatctcccg
     2701 taatacggac aacgtttgta ccatctgcaa acaacacagc acgcttacct tgagccacaa
     2761 caatgccggt accagactga gttttgaacg tggttgtgaa ggctccagtg ttgttgcaga
     2821 aaacaatacc ctgccaatca ttgggaacaa tcacatttct gttaccagtc aaagctccag
     2881 ttgtggttag gtatccgcac cgagcttctg cggcagacaa ggtgtagtca gcagtggtca
     2941 cagcaacact ggctttgctc gtaacggcca caggctgaac ccatggtctg taatcggtgt
     3001 agtcactgac cgttgaagtt gaggtaaccg caaggtatag cggaatacga cccggagtaa
     3061 aaccagtggt attggcacta accacacctg cgcgggtagc ctcgatgtag ttgttgctgc
     3121 tggcagtaag tgcgagggcc gctgcattgt ttgcaatcgc agtcaacacc ccatcaacca
     3181 acatgtatcc accgtagtag aaccagttca aacctgaaca aagagatgct cgcctaccaa
     3241 aaagcatggc cggactcgca gcatcaaaca ggttgttggc agtggtctct ttgctacttt
     3301 gcgactgata aatcagatca agtagcgatg ttttattact cattttgtta tctcgtgatt
     3361 gattgggtta gtgggtagcc gcgaccaaca acttccgaca tctggtatat ctttaaatac
     3421 aaggtcgatt ggttactgcc aaaatctgct atttgattcg aactactgta aatggtgttt
     3481 ggtgatgttg tggaaatggt gcgcttcaca gaagtgtatg ttccatcaga gaacacatct
     3541 atttggtatg cttcagaagt ttcacccaac ggggcatcca cactgtccat ccactcagca
     3601 ttcgctctgg tccgacgcaa ccacgaaaga ttccagtcaa aaccgttgtc tctatctccg
     3661 gtaagataaa caggagagag tggttttaga ttgattgcgt tgtaggagaa agacagatta
     3721 gaatccgtgc taatgtctcg gtcgaaagta atccctctat acaacctaga aagccatata
     3781 accgaactac tcatgctcag caaagatagc gtcgtggaat caagcaggac aacgctatca
     3841 ccattgacat gagaacccat gtactgctct gtcccatacc taccacgaag caggtttgtc
     3901 aacgtatagg ttgttccact caccagagta catgtttgtg cagcgatgat ttcccacctg
     3961 ccgttaactc cataggcaaa atggtttcct cctgccaaca ttgccagttc tgtgatgctg
     4021 gacaaagaac cttgggtgag ttttaccgac aaagaactac cgctgtcctt catacgctgc
     4081 tcaacagcac cgatcgagtt cgtggctata cccatggttg aaccaggggc accaacatca
     4141 taaatatctg cccacgtcga accagagtcc gtgctctgga acaacgagcc acccttccaa
     4201 ccggctttga ctcccgtcat tgccaacaac aaacaaggat cggcttgaga gtcagacatc
     4261 ctcggaagat cgagcaagag gtatgtagat gcacccacag ggacaagcgt tgtttgccca
     4321 gtaacggcac cggcaacgcc aacggcattt ggggtgtaaa cggctgcgac gttgaccttg
     4381 gccttgcatt cgagcctgtg gtcgcttgtg taactgatgc cgactaagcg cacagcaatt
     4441 acaccttcag gtgttggtag atttacaaca tctgcaggtt ccaatgcgtt gtaggttgat
     4501 gggaggttga actgtatgtc ggtgcgttcc aaccacgctg aatagagaat gatctgagcc
     4561 tttccggcgg catctgttga gttgaatacg atcggaagat caactgtctt ttcgttgatt
     4621 gcggatgtat ttaacctttc tgcgtattga gaaccaacgt catactcgcg atccatgtcc
     4681 aggaatttga caattacacg tctcggtaac tggctgtcca cctctcgctt tgtagtgatt
     4741 tgaactcctg gtttgtcttt tgacgaatgt gcatccaagt ctgccgcagc aatggtcatc
     4801 acagcagaat tcccacgcgg gatgaacttg atcttgtacc ctctttgaac cacatcaaac
     4861 gggaaagctg tttgtagtgg ttccagtgag tttctgatag aaccctgttg accgattctg
     4921 tatccagtaa catttgaaga aagcaaggtc gtatcaatgt ctccagagtt tagattgcca
     4981 gaaagcaaac actcaccaga tactacttgg gataaagtag gttgaatcgc agaaatgaag
     5041 ctcggggtaa cgagaattcc tttggtggtg gttggaacca gcagcagaaa ttgtgcgccg
     5101 ttgaaacatg ggtaggagta ctcaccggac ggggtgacgg ccgtgtaact ccatgtgacg
     5161 ccatctggcg acgtgtagta gcggccgttc gatccagcga gaaagactct cccatctgtt
     5221 gccaacccgt ttaggttcgt aggaaacgat tgtgtggtct tcgtccacgt gactccatct
     5281 ggtgatgtac atgttggtct accaccatca tcactggcga cacaaaacag gtttcccttc
     5341 gctgtcgcgc tgttccagtt gattccgccg ccagggtcag tataggctag tgtccatgtt
     5401 cccgcatttc cactgggcga tgtccaaaat tgggagttgt gcacggcgag gaatttagag
     5461 ccattccacg ccaagcagct tgttacccca ctgtatggtg atgtttggga ggaccatgtg
     5521 attccgtctg gcgaggtctc gaatgggaca tagcctgcgt cgtcagttcc gacgaggaat
     5581 ttagcgccgt tccagatgat gcgtgtgtgg tatccagccg tgacgctcgg aaactggaaa
     5641 tatcgtttcg tccagttgat tccgtctggc gaggtgtagc agtaattaac gtgccaatcc
     5701 agcactagca caacgaacag actgccgttg cttgcgatgt ctgcccagga gttggtttcc
     5761 ggcatcgtcc gtggggtcca ggtaacgcca tcgggcgaag tccagcatac accggaggat
     5821 gtcccagaga tggcgcaaaa aacgctaccg ttccaagcgc tgcgaccgaa atagtagcca
     5881 cccggcgtgg tgacggcgtt gtaggtgtaa ctgtaggttg cgcctagcgt gaggacttcc
     5941 actttcacct gagccccagc caaactgttt ccatagttgg ccaactgcaa atcatagaag
     6001 acgatgtacg ccaagccgcg ccatgctgga gtgttggcaa caccaagtgt ggcctgcata
     6061 cgagtgtctg gttgttgggt gtctgttccc gtgtagacaa caaaaccact ggcagcagaa
     6121 ttgctcgctg cgatcgtgct tggatcactg ctcgtagcat cgtagatgag tgttggacct
     6181 atccaaatgc ggcgaacacc aacgatcggg cctttgcaca atccgacagc gaaggttgca
     6241 gagtaggtag ttgtgacagt ggttgtcttt tttccaccac ccttaccacc accggatttt
     6301 ttcttcgtga cagttgcctt taactggttg ttctctaacc aaaagatatt gccaacaaca
     6361 ctgaatgctc cgtagccacg aggaattgca gcaccatagg tgctggtttg cacagtgagg
     6421 tcggacagtg ttggaccata ggtggttgga ccttttggtg ggttaataga tccaccaagg
     6481 agcataccca gttgagcgcc taaaaatggg tatccagcaa aaaacccaat cacaccacca
     6541 acaatagcac cagcaatttg cccagcagaa ctcatacggc atccttaaat ctatagattc
     6601 gaacaatacg agataaccag cgatcatcca gaccatgttc aacacacgaa ccgattgttt
     6661 catatgcatg aataatggtg ttgtcttcag ttaggatagc caaatgctgt ggttctgaga
     6721 aaaagcgcat caacagaata tccccaggtc gcatgtcctc aacaaccatc aaataatcct
     6781 gcatgtccaa cgcttgttcc aacatacctt tgttgggagt ttccccgtac ccctgcacct
     6841 caaccacatc gagtccgaaa gagcccgcta catggcaaca aacaccagcg cagtctaggc
     6901 cgacaccaac aacacgacct tgatgcacaa aaggtgtacc caagcactgt ctagcagcag
     6961 caacaatctc actggtaagc attagcctgt tccaaatgtt gcgtatgtgc ttccggttgg
     7021 aatccacgga aagccaccaa aattgactat gttacttcca ccgactcgcg cttgacacgc
     7081 tgcaattgtc tttctgcacc cacgcaccat gctataggta ttacccacag cagggagata
     7141 gtaaaacggt tcgaacacct ctatcactcc accagaaaac gatttgattt cttgtggctt
     7201 aaatcctgcg tttggtccgc ttgtgaattg aattgttcct gcaccaaaag tatctgctgc
     7261 ttccaatcgg cttgaatctg tgaaaataga accactagcc acggctgtta gcgttccagt
     7321 cactgtgttt gcagcaaggt tcgctttgca cccgccatat tcttggccgc agaaaacctt
     7381 cgggcacgaa ggtgtgtaga tgtccccaac agattggttc aacgcatcta ccaatgacat
     7441 accatttatg gtatatcgat tgtccatcaa agaagcttgt ccaaaaatcc cagcaacgat
     7501 cggttcttgg tcttcaacgg gattgctcca cgtggtggca aaggcatagc aacgggcacc
     7561 gtcaaaaagg ccgctaccga tctgagcacg cgaaatacca gcagcaccag cgattccttc
     7621 taggtcaatg gaggaaggag aaaacccggc ctctgcgttg taacctgaga actgataccc
     7681 gttggtgctc aagtaggtgt gcgaattcat caccagattt gttgggtaat ctgttagata
     7741 aataggcgag cctgttgttg gcacaatgcg catgcacaac acgcgaaacc tgtaatctgc
     7801 aactgacgct ttcatggtga caacaactcc acaatatcga tgcttccaca atctcgcaaa
     7861 tcgctcgaaa gtgcgtgact tgcgaaactg gaatcaaatc tacacggtag gtcgaattcg
     7921 aaccctgcag tgattgtatc tgcgggatac gagggggcag gggatatggt caccagacca
     7981 gttgttgtgt ccacactcac accggaactc aacagggtac cgttcttggc gacaatcacc
     8041 gtgcccgcca caggcttgta gatcgtccta gatgggcgac ccacacccaa accaggggca
     8101 ctcaggccgt acaccttgcg cagttgatat accccactcg atacatactg caaagtctgg
     8161 tcatcttttg tcggggagga cataccgtca acagcactgg tgaaatcgtc aaggcatttg
     8221 actcgaaacc ctgcaaactt accaaacaca cggtcgtaca ggtccttcac cagtcgaccc
     8281 atgttgtctc tggtcagggt gaagttgatt gtccacttgc gtttcgggaa accatgacgc
     8341 aacacccggt actcggcatc actcgctgtc gttgtgattt caaccgaata gtcctcagtg
     8401 taactagcac cctctcgaac attcaacgag agcctttctt ccaaaaaact agccatggcg
     8461 ctgagccctc ctcattacat taagcacgct ctgcattcct tgcgtggccg atctgcgaac
     8521 atcggacggc gattccatcc cgtagatgtt catggtgata tgcgtgctgt caccggcacc
     8581 actgagagt
//
LOCUS       DAOQLC010000153         8426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3361_length_8426_cov_23.618206, whole genome shotgun sequence.
ACCESSION   DAOQLC010000153 DAOQLC010000000
VERSION     DAOQLC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3361_length_8426_cov_23.618206"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PL000_18620"
     CDS             <1..219
                     /locus_tag="PL000_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90953.1"
                     /translation="PPAASGTLFADFPSTDPSYWAIDWAEQAYNDGLLPACGTDSATG
                     KPMFCPSQLVDRGWGAYLIVKAKNLPLP"
     gene            386..3556
                     /locus_tag="PL000_18625"
     CDS             386..3556
                     /locus_tag="PL000_18625"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNC90954.1"
                     /translation="MKTQNRISPTVKALRGIVLLAVLFSMIQAQPASAQVTGQGLEIC
                     LLPGTVQLKQDESQALKSALRKADAEVEFNGAVCFAPIELREEGEWMFITLAGLTNKP
                     GGAWTISEDAVWSDNAIIRKTPGGMLASVAGTKAFSAALSSVPESIVPLEAKRVIDPL
                     SAYSPSPHAGAAFQFPFEAGYQVYYGTKGGGVHTGEFSTVLGGTSMAVDLMSDGITAS
                     NHAPNRLLAVAPGTVVKKCSDATKNSTYLIIDDGTDKFLYAHIRSSTAPPLWAVVNVA
                     GTILGELVPGSFSDGYCGYANQTAGFFHVHLEFLNRSSLTLSDWTLDTATEKWQRGAE
                     VWSAGEWKQVGGSVTGCSQVSYTGVILYENANCNVETAGAMKAFPAAITWTDIAVADP
                     IQNKASSIFVSSGWSAKVFEKSIAEGGGSWRCVTDRLSDLSTSYYTNGDTSRKMNDDI
                     SSIQVYADGNCGSGGTSDWTALYFNGHDHWLDTSNFNNQMCQETITSSGLDKNYTSSA
                     PCNNGVANDWVAEYNTTVNFASGNYVFKAENDDGLKLWVNGVSVLDRSAAQGLSYACP
                     ARSLSGNVPIRAILNEETGDARIKIAWTTDVNVCNLPGAFGKVGPVNGATAQPNNPTL
                     SWNASVDATEYFYCIDTSNDTVCDTGWQSVGNVTSQAISGLAVDKTYYWQIRANSAGG
                     ETFADGNTWWSFKTQPSNMLINPGFEDGTTTPTAWNKDAFVLANSTFEWSIDFFHSGS
                     RSVKIISATANDARWTQTVTVQPNTDYKLSGWILTNDVAHTAETNDAGANLSILDGTV
                     NHSTPSLFGDNPWTFVSLTFNSGANTQVTVAARLGMATGSTTGTAWFDDLKLEPATSA
                     PTFGDVPLDHPQYAYIQALWDNGYTAGCSTDPLLFCPDTILDRAQSAVFMLRGQLGST
                     YAPPAAPWNTFGDDWTGFEWAEPWAEGMWQEGLTQGCQASPLLYCPSTQLPRVEASVF
                     GLRMKYGVNYTPPTATGTLFADFPSTDPSYWAIDWAEQAYLDGLLPACGTDSVSGKPM
                     FCPSQLVDRGWGAYLIVQAKGLLPTP"
     gene            3983..5356
                     /locus_tag="PL000_18630"
     CDS             3983..5356
                     /locus_tag="PL000_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC90955.1"
                     /translation="MKKLHRSFRLLVILFVLVNLLPSYNSLAAPLAADYPTDPTADIS
                     WSAGTNGVADVQAAFNQARAVESGQLGISLPALTLPSQADWDALSDGEKSLWLINRER
                     SDRGVDPLEDVELNVTSVAQTYADFLLDNDAWGHTEDGRDPWERLDSNPAIQACHDFL
                     GVSENLAVFVTSGTSISLPIERAVYMWMYDDSGSGWGHRHAILWSSYTDSHGSSGSEG
                     FLGIGRASGGPYQGPFSQPWLHAELIVMNVFDPCAGYSVPQATFGDVPVDHPQYKYIQ
                     ALYDGGYTAGCSTSPLMFCPDTIMDRAQAAVFMMRGQFGSGYTPPPAPWDTFADDWTG
                     FEWAEPWAEGMWQEGLTGGCRQDPLMYCPETDLSRVEATVFGLRMKYGVNYEPPLATG
                     KVFADLTDPTYWGAGWAEQAYADGLLPECGFDNETGKPYFCPLDLVNRAWGAYLIVKA
                     KNLLPAP"
     gene            5590..7158
                     /locus_tag="PL000_18635"
     CDS             5590..7158
                     /locus_tag="PL000_18635"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC90956.1"
                     /translation="MNKRSYLPVVLIMLIVMVFGGHTNAYASNPAAAKSARVEVNGVS
                     TPCTTFKTCDDLLGPDATLYISGEWNEQLRVTESGTAGHPIRVIGTDAIIKVDKTEVS
                     GIALTGNYINLSGFEVIGGDSFGIYITGQVIKVENNIVHDSVLENGTAGNCRQGSWGS
                     AIKVREAMYVEVRNNQVYHSCGEGIALRGTYVLAEYNTVWDNFSVQLYVDNSPYTTAQ
                     NNTVKCTPGFPTPRQPVGIAIGEERFSGWGNQRHDNKVLNNYVDGCADGIVSWEGEMS
                     TAKFIDSVISGNTVVNGNGRAIGLWSKNQNVLIENNTIYSEVFIKYPEGVTSINNTVI
                     GSTPSIFSDVPATYWAVSYIESLYNAGITGGCSTSPLLYCPTSSVTRAQMAVFLLRGI
                     HGSGYTPPAATGAVFGDVSTSTFAAAWIEQLAAEGITSGCGNGNYCPDATVTRAQMAI
                     FLLRSKYGSAYVPPVATGVFPDVPVGSFAADWIEQLAAESITSGCGGGNYCPNSSVTR
                     DQMAVFLVKTFNLP"
     gene            7232..8083
                     /locus_tag="PL000_18640"
     CDS             7232..8083
                     /locus_tag="PL000_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90957.1"
                     /translation="MKTQNQSIPQFDLGGNGPSLHFLHANGYPPECYLPLFELIKTQY
                     HLFGMKLRPLWPDSSPKEISDWKPLSDDLLRFLSNRGTDPVIGVGHSIGGIVTLRAAL
                     RAPQKFRAIILLDPVLFVPNFILMWNIVRALGLGNKAHPKIGGALKRRREFDNLDLIF
                     RGYRTREVFKYMSDESLRAYILGITKEKQGGGFELAYSPEWEARIYLTGLRDLDIWHR
                     LPKLEVPTLIIRGAETDTFLEAAAMLVKQKQPKVKIESLPESTHILPLEYPQKVFEIM
                     QTFLNDL"
     gene            8218..>8426
                     /locus_tag="PL000_18645"
     CDS             8218..>8426
                     /locus_tag="PL000_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90958.1"
                     /translation="MSHIKLSWRNIIIAATLPAVMEVIWQLYNTYVPVYLQAGNPAFT
                     DAGATTVGFGLGPALTGFILVFDNIA"
BASE COUNT         1984 a         2073 c         2203 g         2166 t
ORIGIN      
        1 ccgcccgccg caagtggcac gttatttgcc gatttcccct ccacggaccc gagctactgg
       61 gcaatcgatt gggcggaaca agcctacaat gatggtctgc tccccgcctg cggaacggat
      121 tccgcaacag gcaagccgat gttctgccca agtcaactgg tggatcgcgg ctggggtgcg
      181 tacttgattg tcaaagccaa gaacctgccg ttgccgtaag tttgccagac tagatcgaat
      241 aaaaaagggc agtcactgaa aaagtggctg cccttttgtc atgcaaatac gatgaatctc
      301 tcccaatttc tgacttataa aatagatgca ggtattgcgc atttgtgcgc agaagtttgt
      361 tattttgcca gaatggaagg ttcatatgaa aacacagaat cgaatttctc caacggtgaa
      421 ggccttgcgt ggaattgtgc ttttggcggt tctcttttcc atgattcagg cacagcctgc
      481 cagcgcgcag gtcacagggc agggattgga aatctgcttg ttgcctggta cggtgcagtt
      541 gaagcaggat gagagtcagg cgctcaagtc tgcgctgcga aaggcggatg cggaagtgga
      601 attcaacggc gccgtatgct ttgctccgat cgaattgcgc gaggagggtg agtggatgtt
      661 catcaccctt gcagggttga cgaacaaacc aggcggtgct tggacgatct cagaggatgc
      721 ggtctggtca gataacgcga tcattcgcaa aaccccgggc ggaatgctgg cttcggttgc
      781 aggtacaaaa gccttttctg cggctctttc ttcggtgcca gaatcaattg tcccgttgga
      841 agcgaagcga gtgatcgatc cattgtctgc ctactcgccg agtccacatg cgggggcggc
      901 attccaattc ccgtttgagg cggggtatca agtttattat gggacgaagg gcggcggtgt
      961 tcatacgggc gagttttcta cggtgctggg cggcacgagc atggcagtgg atttgatgtc
     1021 ggatggaatt acggcttcaa accacgcgcc gaatcgtttg ctggcggttg cgccgggcac
     1081 ggtggtaaag aaatgttcag atgccaccaa gaattccact tatctcatca ttgatgatgg
     1141 caccgataaa tttttgtatg cccacattcg ttcatccact gcgccgcctt tgtgggcggt
     1201 ggtgaatgtg gcagggacga tcctgggtga gttggttcca ggttcgttca gcgatgggta
     1261 ttgcggttac gccaaccaga ccgcaggttt cttccatgtt catctggaat tcctgaatcg
     1321 ttcttcgctc acgctcagcg attggactct cgatacggcc actgaaaaat ggcagcgcgg
     1381 tgccgaggtg tggagcgctg gtgagtggaa gcaggtgggt ggcagcgtaa cgggatgcag
     1441 tcaggtttca tatacgggcg tgatcttgta tgagaacgcc aactgtaatg tggaaaccgc
     1501 aggggcgatg aaagccttcc cggctgcgat cacgtggacg gatattgctg tggcggaccc
     1561 gatccaaaat aaagcatctt cgatcttcgt ctcttcgggc tggtcggcta aagtatttga
     1621 gaaatccatc gctgaaggcg gcggctcgtg gcgatgtgtg acagatcgtc tttctgactt
     1681 gagcacaagt tattacacca atggcgatac atcgcggaag atgaacgatg acatctcgtc
     1741 cattcaagtt tatgcggatg gaaactgcgg cagcggcggg acctcagact ggactgccct
     1801 gtatttcaat gggcatgatc attggctgga tacttccaac ttcaacaacc agatgtgcca
     1861 ggaaaccatt accagcagcg gcctcgataa gaactacact tcctccgctc cctgcaataa
     1921 tggtgtggcc aacgattggg ttgcggaata caacaccacg gtgaattttg cctctggaaa
     1981 ttatgtcttc aaagccgaga atgatgacgg gctcaaactg tgggtgaatg gcgtcagcgt
     2041 tcttgaccga agcgccgctc aaggcctttc gtatgcctgc ccagcccgca gcttaagtgg
     2101 gaacgttccc atccgtgcaa tcctgaatga agaaacaggc gatgcccgca tcaagatcgc
     2161 atggacgacg gatgtcaatg tttgtaacct gccgggcgcg tttggcaagg ttggccctgt
     2221 caacggtgcc actgcacaac ctaataatcc caccttgagt tggaatgcca gtgtggatgc
     2281 aacggaatat ttctactgca tcgatacttc gaacgacact gtttgcgata cgggctggca
     2341 aagcgttggc aatgtgacca gtcaggcgat cagtgggttg gcagtggata agacctacta
     2401 ttggcagatc cgcgcgaaca gtgcaggtgg tgagaccttt gccgatggca atacctggtg
     2461 gtcttttaaa acccagcctt ccaatatgtt gattaaccca ggctttgaag atggcacaac
     2521 cactccaact gcctggaaca aggatgcctt tgtgttagcc aacagcacct ttgagtggag
     2581 cattgatttc ttccactcag gcagtaggag cgtgaagatc ataagcgcca cagccaacga
     2641 tgcgcgctgg actcaaactg taacggttca gccaaatacg gattacaaat tatctggctg
     2701 gatcttgacc aatgatgttg cccacaccgc tgagacgaac gacgctgggg ccaatctctc
     2761 cattttggat ggtacggtca accactcaac cccgtcactg ttcggcgata acccgtggac
     2821 tttcgtgtct ttgacattca actccggagc aaacacgcaa gtgacggttg ccgcgcggct
     2881 tggcatggct acaggctcaa caacaggcac cgcctggttc gatgacctga aattggaacc
     2941 agcaacaagt gcgcccacct ttggcgatgt gccgcttgac catccgcaat atgcctatat
     3001 tcaggcgttg tgggataacg gctacaccgc aggatgcagc acggatccgc tgttgttctg
     3061 ccccgatacg atcctggacc gtgcccagtc tgcggtgttc atgctgcgcg gacaacttgg
     3121 ctcaacctat gctccgcctg ccgcaccgtg gaataccttc ggcgatgatt ggactgggtt
     3181 cgaatgggcc gagccgtggg cggaaggcat gtggcaggag ggattgactc aaggttgtca
     3241 ggccagcccg ttgctgtatt gtccttccac acagcttccg cgagtggagg ccagcgtatt
     3301 tggtctgcgc atgaaatacg gagtcaatta caccccgccc acagccacag ggacattgtt
     3361 tgccgatttc ccatccacag acccgagtta ctgggccatt gactgggccg agcaagccta
     3421 ccttgatggt ctgctccccg cctgtggcac cgatagtgtc agtggcaagc cgatgttctg
     3481 tccaagtcaa ctggtggatc gcggctgggg tgcgtatcta atcgtgcaag ccaagggatt
     3541 attgccaaca ccataatccc tgcttttctc ctttgagttg cagcgcgaga cccctgggtc
     3601 ttgcgctgca cgttttaacg ggtttggtta tggatactcc tgtaactact ccatatttcc
     3661 attgttcatt ttttacgagt agaactcttg cataagtcca tttctttacc accaaggcac
     3721 aaagtctcga agactcaaag aaaaaaagct tagtgccttt gtgactttgt ggtctacaac
     3781 actttactta tgcaagaggt caagtgtttt tttgagtgca acagaacgtc cattgtgaaa
     3841 ttcatattga aataaatttt tatttttatt gaagaataaa attattttgc gatgtacgct
     3901 taacaaacct gtgggtttgg gaatttataa aaaatggtat atgtagccat cattgttgca
     3961 gaaatatcca cgggagttaa gtatgaaaaa actacatcgc tcatttcgcc ttttggtaat
     4021 cctttttgtg cttgtgaatc tattgccttc ctataattca ttagccgctc cgttggcggc
     4081 tgattatcca accgatccaa cagcggatat cagctggtcc gcagggacaa atggtgtggc
     4141 ggatgtgcag gctgcattca accaagcgcg ggctgtggag agtggtcagc ttggtatctc
     4201 attacctgca ttgaccctgc ccagtcaagc ggactgggat gccttgagcg atggcgaaaa
     4261 atccctgtgg ctgatcaacc gtgaacgaag cgaccgtggt gtcgatccgc ttgaagacgt
     4321 ggaactgaat gtcaccagtg tggcccaaac ctatgctgat ttcctactcg ataatgatgc
     4381 ctgggggcac actgaagatg gccgtgatcc gtgggagcgc cttgattcca accctgctat
     4441 tcaggcttgc catgatttcc tcggtgtttc agagaatctt gcggtttttg tcaccagcgg
     4501 aacgagtatt tctttaccga tcgagcgcgc tgtttacatg tggatgtatg acgacagcgg
     4561 ttctggctgg gggcatcgcc atgccattct ctggtcttct tatactgata gccatggctc
     4621 ctctgggagc gaaggctttc tcggcattgg ccgtgcatct ggcggaccgt atcagggtcc
     4681 attttcacaa ccctggttgc atgctgaatt aattgttatg aatgtttttg atccatgcgc
     4741 tggttattct gttccgcaag ccacctttgg cgatgtgcca gtggaccatc ctcaatataa
     4801 atatattcag gctttatatg atggcggcta tacggcaggg tgcagcacca gtccgctgat
     4861 gttttgcccc gatacgatca tggatcgtgc ccaggcggca gtcttcatga tgcgcgggca
     4921 gtttggctca ggctatacac ctccacctgc accatgggat acctttgctg atgattggac
     4981 gggttttgaa tgggcagagc cttgggccga agggatgtgg caggaaggcc tcacaggtgg
     5041 gtgtcgacag gatccgctga tgtattgtcc tgagacagac ctttcacgtg tggaggcaac
     5101 ggtcttcggt ctgcgcatga agtatggagt taactacgag ccaccattgg caacgggaaa
     5161 ggttttcgcc gacctcaccg atcccaccta ttggggcgca ggttgggcag agcaagccta
     5221 tgcagatggc ttgctcccag aatgcggttt tgataatgag acgggcaagc catatttctg
     5281 tccgttggat ttggtcaacc gtgcctgggg ggcgtatctc attgtcaagg ccaagaacct
     5341 gcttccagcg ccgtaagatc gtttcaacat gggcggaatc atgccaaaag gcgattcctt
     5401 acattgggga gcggtcattt gacggggttt tcgtccaact aacaaaattg taggtttttt
     5461 tgaaatcttt ttgagcacca atggatactg tttaatgtat cattttcata tcaaaacgcc
     5521 gaggcggctc gatttgcttc ggctttaatt ttggacatcg aatttttcta tatttaataa
     5581 ggaaagaaaa tgaataaaag atcatacctc ccagtcgtcc tcattatgct gatcgtcatg
     5641 gtgtttggcg ggcatacaaa tgcgtatgcc tccaaccccg ctgctgccaa aagcgccagg
     5701 gtggaagtta atggtgtatc cacaccatgt actacattca aaacgtgtga cgatttgctc
     5761 ggccccgatg ccacactcta catttcagga gagtggaacg agcaattacg tgtcaccgag
     5821 tcgggtactg cgggtcaccc cattagagtc atcggtactg atgcgatcat caaggtcgat
     5881 aagaccgagg tgagcggaat cgccctaaca gggaattaca tcaacctctc tggttttgag
     5941 gtcattggag gcgactcctt tggcatttac atcacagggc aggttattaa agtggaaaac
     6001 aacattgtcc acgatagtgt gcttgaaaat ggcacggctg gcaattgccg ccagggcagt
     6061 tggggtagtg ccattaaggt tcgtgaagcg atgtatgtgg aagtacgcaa taatcaggtt
     6121 tatcacagct gcggtgaagg gattgccctg cgcggaacct atgttttggc agaatacaac
     6181 accgtgtggg ataacttctc agtacagctt tatgtggata actcgccata caccactgcc
     6241 cagaacaata ccgtcaaatg caccccaggc ttcccaaccc cccgtcaacc tgttggaatt
     6301 gcgataggcg aagaacgctt tagcggctgg ggcaatcagc gccacgataa caaggttttg
     6361 aataattatg ttgatggctg tgcagatggc attgtttcgt gggaaggtga aatgtccact
     6421 gccaaattca tcgactcagt gatttctggc aatacggttg taaatggaaa tggacgtgcc
     6481 atcgggttat ggtcaaagaa tcagaacgtg ttgattgaaa acaatacgat ctactccgag
     6541 gtgttcataa aatatcccga aggcgtaact tcgatcaata acaccgtcat tggctcaacg
     6601 ccttccatct tttcagatgt tccagccaca tattgggctg taagttatat cgaaagtttg
     6661 tataacgcag gcattactgg tggatgctcc acatcgccgc ttctgtattg cccaacttcc
     6721 tctgtgaccc gtgcgcaaat ggcggtcttc ttactgcgtg gaattcatgg cagcggctac
     6781 actccacccg cagcaactgg agcagtcttt ggtgatgttt ccaccagcac ctttgctgct
     6841 gcatggatcg agcaattggc ggctgaaggt atcaccagtg gatgcggtaa cggaaactac
     6901 tgtcctgatg ccaccgtcac ccgcgcacag atggctattt tccttttgag gagcaagtac
     6961 ggttctgcgt atgtgccgcc tgtggcaact ggtgttttcc cggatgttcc cgttggctcc
     7021 ttcgcagcag actggatcga acaattggcc gctgaaagca tcaccagtgg atgtggcggc
     7081 ggaaactatt gtcccaactc gtcggtaact cgcgaccaaa tggctgtatt ccttgtgaag
     7141 acctttaatc ttccataggt ttaaactaat ttgcaaattt atgaagcctt cacaacttgt
     7201 gaaggcttca tattatttat aatcaaaaca aatgaaaact cagaaccaat caatccccca
     7261 gttcgattta ggcggtaatg gcccatcttt gcacttcctt cacgccaacg gctatccccc
     7321 cgaatgttac ctgccgctct ttgagcttat caaaacccaa taccatcttt ttggaatgaa
     7381 acttcgcccg ctttggcctg attcaagccc gaaagagatt tccgactgga agcctctctc
     7441 cgacgacttg ctgcgcttcc tctctaaccg tggaactgat cctgtaattg gggtgggtca
     7501 ttccatcgga ggcattgtca ctttacgcgc cgccttgcgc gctccgcaga aatttcgcgc
     7561 catcattttg ctggacccag tcttgttcgt gccgaacttt atcctgatgt ggaatattgt
     7621 ccgtgcattg gggcttggaa acaaagccca cccaaagatt ggcggcgcac taaaacgcag
     7681 gcgtgagttc gacaatctgg atctgatctt tcgggggtac cgtacccgtg aggttttcaa
     7741 atacatgagc gatgaaagtc tgcgggctta tattttgggt attacaaaag aaaaacaggg
     7801 tggtggtttt gagcttgctt attctcctga atgggaagcg cgtatttacc tcacaggcct
     7861 gcgcgacctc gatatctggc acagattacc caaactcgaa gtgccgaccc tcatcatccg
     7921 cggcgctgaa acggatacgt ttctggaggc agccgcaatg cttgttaagc agaagcagcc
     7981 caaggttaag atcgaatcgt tgcctgaatc cacgcatatc cttccgttgg aatatccgca
     8041 aaaggtcttt gagatcatgc agacatttct caatgatctg taaaccctcg aaaggtcact
     8101 cgaagcttcg gaattccaaa aaaacctact tgactcgcaa ccttttttct tgtatgctgt
     8161 tttaacgtta gaacaatttt ttgaacgatt gttcaaaacc tcacaggaga agagaaaatg
     8221 tcccacatta aattgagctg gcgtaatatc atcatcgcag cgacactgcc cgccgtgatg
     8281 gaagtcatct ggcagttgta taacacctac gtccccgtct atttgcaagc gggcaacccc
     8341 gccttcacgg atgcgggagc caccaccgtc ggattcggtc tcggacccgc actgacgggt
     8401 ttcatcctcg tcttcgataa catcgc
//
LOCUS       DAOQLC010000154         8379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3397_length_8379_cov_28.475372, whole genome shotgun sequence.
ACCESSION   DAOQLC010000154 DAOQLC010000000
VERSION     DAOQLC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3397_length_8379_cov_28.475372"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PL000_18650"
     CDS             complement(<1..778)
                     /locus_tag="PL000_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90959.1"
                     /translation="MADMIKCPVCGENNRLGQEFCLNCQTRLGPVPGSSKGDETLKPG
                     QAPTKKNTADLEPILPQWLKDARDSARNIGSGDVPAEPSTPKQTQPASPVDFLAGLHS
                     QADDNDEEDIPDWLANITGDAPKSKKSQPEPSEVRWVEMGEKNDFVQDEQPAAESDTP
                     PWLAGLASNAPQTEKDELTDWFRDASGIQKPAEAAPSSSVDSPDWLRSMAAEDGQGGF
                     TSSSEPADTFGNISASADDTPDWLRAMAADNEAKNNSQPAS"
     gene            complement(778..1395)
                     /locus_tag="PL000_18655"
     CDS             complement(778..1395)
                     /locus_tag="PL000_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90960.1"
                     /translation="MRIFSVIFAFIAITAGVLVLLGYFIPAFSVIQVLLLNWAVILAG
                     VAVIVGVFNLALVHSDKVRRGEKGSVYSALLVVSLLATLIFGILLRPGHTIMKMVVNG
                     IIIPSEAALMGLLTISLLYAAIRLLRRRVDLMSIIFLITAAFVLLGTATLTFGGIPLI
                     GEASQWVTQVLALGGARGILIGVSLGILMTGLRVLFGIDRPYGGN"
     gene            complement(1395..2132)
                     /locus_tag="PL000_18660"
     CDS             complement(1395..2132)
                     /locus_tag="PL000_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90961.1"
                     /translation="MKIIELAILAVSALLLLAVTFWKRRSPANLRDIPALTRLARTLG
                     LSIEDGTRLHISLGRGSFLDVRGGSGLAGLAVLRHIAERTSVSDEPSIASAGDPALGL
                     LAQDTLQAGYHAAGVEELYVPTTGRVTGLTPFGYAAGAMNISQHENVSTNILLGHFGP
                     EVALLAETADRENITVIGASDDLTGQAVIFANTQDALIGEELFAAGAYLGAGVSHAAS
                     LTVQDILRWFIILALMGGAFAKFAGMI"
     gene            complement(2129..2386)
                     /locus_tag="PL000_18665"
     CDS             complement(2129..2386)
                     /locus_tag="PL000_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90962.1"
                     /translation="MLYSLYMKPSREYWPRWAETLRRYQLTEFAASLLEAGSPLALLG
                     AQALYFSRGLFESDQLTALAATLEEDGQAFASYLVEERASL"
     gene            complement(2454..3704)
                     /locus_tag="PL000_18670"
     CDS             complement(2454..3704)
                     /locus_tag="PL000_18670"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNC90963.1"
                     /translation="MPSLNEVLAVILGGGRGARLYPLTQMRSKPAVPIAGKYRLIDIP
                     ISNCINSGIYRIAVLTQFNSVSLHRHITNTYHFDSFHPGWVQIWAAEQTIESADWYQG
                     TADAVRKQTLEIQSSGAKNVLILAGDHLYRMNYNEMAEYHWNSKADITVAVQPVPRDE
                     ASRLGILKREKNGRISAFVEKPKDVEVQNKFISRDDAVRPFLGSMGIYMFKTRVLMEL
                     LSNFPSFHDFGGDIIPQAIQSYSVYGFDFSGYWQDIGTIRSFYETNLALTTSETPFNF
                     YDPKLPIYTDTRFLPGSIVEDSKIRDVLIAEGSRIQKSDITHSIIGIRSQISRGCIIK
                     DTIMLGSDYYERDDEGLPIGIGENCHIEGAILDKNVRIGSDVVIRPFPRNTDIDNAKW
                     YVRDGIVVIPKDMVIESGTKIGPE"
     gene            complement(4193..4275)
                     /locus_tag="PL000_18675"
     tRNA            complement(4193..4275)
                     /locus_tag="PL000_18675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4239..4241),aa:Leu,seq:caa)
     gene            4329..5387
                     /gene="ychF"
                     /locus_tag="PL000_18680"
     CDS             4329..5387
                     /gene="ychF"
                     /locus_tag="PL000_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNC90964.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRSNTPTSASAGRIEVHTAVVDVP
                     DPRVTKLSGMFNPKKTIYAKVTYADIAGLESGSAKSGISGQLLNQLNQMEGLILIVRG
                     FADDNVMHPNGSIDPLRDVDSMLTELLLNDMIAVERKLEKLTEERKKGGTDKVINARQ
                     TELFEKLHATLSDNKPLRSMEFTNEEQKELASYGLLTRKPILTVFNMGEGQTAPTAQL
                     DHPSVALMGKLEMEIAQLSAEDAAVFMEEYGIKELSLNRMINLSYDLLKVQTFFTVGE
                     DEVRAWTTRRGASAAESAAEIHTDLQRGFVRAEVVSYDDLTSLGSMNEAKAKGKLRLE
                     GKEYPVKDGDIMHIRSSL"
     gene            5471..>6426
                     /locus_tag="PL000_18685"
     CDS             5471..>6426
                     /locus_tag="PL000_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90965.1"
                     /translation="MFRERGVWGAILLSLIFLFVLACGISVTPNTSSPPAFDPTKAAL
                     ELQATSMSLQLTQAALNAQQAAQSTSPPPQPTAAPTEAPAQAQPTPTQDVEARIRAAN
                     VLVYENTDEYGIGMWIQDALDNMGIKYTQTGSYSGHFMEYLNSGTKYDLIIVGAEAKD
                     RISGEFWDVINTRLTRDKVALIAEVWYLDTEANGPISKILGGCGIRYQKDYQLADSIY
                     WWDSTHPVFNEPNTVLPLLHYDRYWANQTGDQIRLGGSGDAVLLAGLAAQKSNEEAVL
                     ATCYEGRVIIQTFSDHDYKMADVIPLWENYIHYTLKNHFLAVP"
     assembly_gap    6427..6526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6590..>8379
                     /locus_tag="PL000_18690"
     CDS             6590..>8379
                     /locus_tag="PL000_18690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNC90966.1"
                     /translation="MAYTQKKWSLAPLYPGYESAELQNAFDMIEEQVTSFEGVRGKLN
                     PEISAEQFMQIVRASEETLRIANKLYSFAGLSFTADTQDQAAQSLQNRAMQFLAEIEN
                     RTMFFNLWWKEVDETNARRLMDAAGDYRYYLEALRLFKPHTLTEAEEKIVNIKNVTGS
                     NALINLYDAITNRYTFKMRVGGKEKEMTAAELQSFRYSTDPKVRAESYQEQFRVYGND
                     GVILGQMYQTRARDWHNENIDMRKFTSPIAARNLNNDIPNEAVDALLNTARKNVKIFQ
                     RYFNLKAKHVGMKKLRRYDIYAPVARSKKTYEFDTAADMVLEAFSAFDPKVAELAKRV
                     FDEDRIDSEIRKGKRGGAFCWSVLPEMTPWVLVNYQGTGREVATLAHELGHAIHSMLA
                     AGHNTFSFHSSLPLAETASTFAEMMLIDKMLAEETDESVRRDILFKQMDDAYATILRQ
                     SYFALFEKQAHEMVQKNASVDELCAAYMENLKEQFGDSVELSDEFKWEWVGIPHIYHT
                     PFYVYAYAFGQLLVLSLYQQFKAEGESFKPKYLKILSAGGSEAPEKILTEAGVDIRSA
                     KFWQGGFDVLEKLVSELEQLPADKPKVKKAV"
BASE COUNT         2239 a         2187 c         2068 g         1785 t
ORIGIN      
        1 tgctggcagg ctggctgtta ttctttgctt cattatccgc agccatggca cgcaaccaat
       61 caggcgtatc atcagctgaa gcggaaatat tcccaaacgt atctgcaggt tcgctggatg
      121 aggtaaagcc accctgacca tcttctgcag ccatggaacg cagccaatcg ggagagtcca
      181 cacttgaaga tggagcggct tcggcgggtt tttgaattcc gcttgcatca cggaaccaat
      241 cagtcaattc atctttttcg gtttgtggtg cgtttgaggc cagaccagcc aaccagggtg
      301 gggtatcaga ttctgcggca ggctgttcat cctgtacaaa atcattcttc tcacccatct
      361 ccacccaacg cacttcagaa ggctcaggct gtgacttttt cgatttcggt gcatcaccgg
      421 ttatgttggc aagccagtct gggatatctt cttcatcgtt gtcgtctgcc tgagaatgca
      481 gaccagctaa aaagtccaca ggagatgcgg gttgggtttg tttgggggtc gaaggttcag
      541 caggtacatc accgctacca atattccggg ccgagtcgcg ggcatccttc aaccattgcg
      601 gcagaatcgg ttcaaggtcg gctgtattct tttttgtggg ggcctgcccg ggcttcaagg
      661 tttcatcgcc tttggatgag ccaggcacag ggccaagccg ggtctggcaa ttcaaacaga
      721 attcctgacc gaggcggttg ttctcaccac aaacggggca cttgatcatg tctgccatta
      781 attgcccccg tatgggcggt caatgccaaa gagaacacgc agaccggtca taaggatacc
      841 cagcgaaaca ccgatcaaaa taccgcgcgc accacccaaa gccagcactt gtgtcaccca
      901 ctgtgaagcc tcaccaatga ggggaattcc tccaaaggtt agggtggcag tgcccagcag
      961 gacaaatgca gctgtaatta aaaagataat gctcatcaga tccacgcggc ggcgcaaaag
     1021 acgaatggct gcataaagca gggaaatggt gagtaatccc atcaatgcgg cttcagaggg
     1081 aataatgatg ccatttacca ccattttcat gatggtgtgc ccaggccgca gtaaaattcc
     1141 aaagataagg gtggccagta ggctcaccac cagtaaggca ctgtagacgc tgcccttttc
     1201 acctcggcgc actttgtcgg aatgcacaag ggcaaggttg aaaacgccca caatgacagc
     1261 cacaccggca aggatgacag cccagttcaa taacaatact tgtatgacag agaaagccgg
     1321 gatgaaatag cccagcaaaa ccaatacacc ggcggtaatg gcaataaagg cgaaaattac
     1381 tgagaagatt cgcatcagat catccccgca aatttggcga atgcgccgcc catcagagct
     1441 agaatgatga accagcgcaa aatatcttgc acagtcaggc tggctgcatg ggaaacgcca
     1501 gcgccaagat aagcacctgc ggcaaacagt tcttcgccga tcaaggcgtc ctgcgtattg
     1561 gcaaaaatga cggcctgccc ggtcagatca tcactggcgc cgatcacggt gatgttctca
     1621 cggtcagcag tttcagcaag taacgcaact tccgggccaa aatgtccaag caaaatgttg
     1681 gttgaaacgt tttcgtgttg ggaaatgttc atcgccccag cagcgtaacc aaatggggtc
     1741 aaacccgtta cacgcccagt ggtggggaca tacaactcct caacacctgc agcgtgatat
     1801 cccgcctgta aagtatcctg cgccagcaaa ccgagcgcgg gatcgccagc agaggcaatt
     1861 gacggctcat cactgaccga ggtccgctca gcgatatgcc gcaacacagc caaccccgcc
     1921 aaaccagagc cgccacgcac atccagaaaa ctgccacgcc caagtgaaat atgcagacgt
     1981 gtaccgtctt caatgcttaa acccagtgta cgagccagcc gtgttaaggc tgggatgtca
     2041 cgtaaatttg cgggagatct gcgtttccaa aaggtgactg ccagtaaaag gagcgcacta
     2101 accgcaagga tggccaactc gatgatcttc acaacgaagc cctctcttca accagatacg
     2161 atgcaaacgc ctgaccatcc tcttcaaggg tggcagccag ggcagttagt tgatcgctct
     2221 caaataaacc acggctaaaa taaagcgcct gcgctccaag caaggcaaga ggacttcctg
     2281 cttcaaggag cgaggcggca aattcggtaa gctggtaacg gcgcagggtt tctgcccagc
     2341 gcggccagta ttcacgcgat ggcttcatat aaagcgagta tagcataaat ttaatgacgt
     2401 ctcgcgcagg tgttgatgac aagtaaattg cagactgaat gaacgaaccc tttttactca
     2461 ggcccgatct tcgtccctga ttcgatcacc atatccttgg gaatcaccac aattccatcg
     2521 cggacgtacc actttgcatt gtcaatatcc gtgttgcgcg ggaatggacg aatgacaaca
     2581 tcactaccga tacgtacgtt cttatccagg atcgcgcctt caatgtgaca attttcgccg
     2641 attccaatag gcaacccttc gtcatcgcgt tcatagtaat cagaaccaag catgatggtg
     2701 tctttgatga tgcatccacg cgagatctgg ctgcggatac cgatgatgga atgggtgata
     2761 tccgatttct gaatacggct gccttctgcg atcaacacat cacggatctt gctatcctct
     2821 acaatggaac cggggaggaa gcgtgtgtcg gtgtagatgg gaagtttcgg atcataaaaa
     2881 ttgaacgggg tttctgaagt ggtcagggcc aggttggttt catagaaaga acggatggtt
     2941 ccaatatcct gccagtagcc gctgaaatcg aagccataga ccgagtagga ctgaatcgcc
     3001 tgtggaataa tatcgccgcc aaaatcgtga aaactgggga agtttgaaag caattccatc
     3061 agcacacggg ttttgaacat gtaaataccc atcgaaccga ggaaaggacg caccgcatca
     3121 tcacggctga tgaacttatt ctggacttcg acatccttgg gtttttctac gaacgcggaa
     3181 atgcgaccat tcttttcgcg cttcaaaata cccaaacgtg atgcttcatc acgcggtaca
     3241 ggctgtactg ccactgtaat atctgccttg ctattccaat gatattcagc catttcattg
     3301 tagttcatac gatacaggtg atccccggca aggatcagca cattcttcgc cccactggat
     3361 tgaatttcaa gagtctgttt acgaaccgca tccgcagtgc cctgatacca gtcagcactc
     3421 tcaatggtct gctctgccgc ccaaatctgt acccagccag gatggaaaga gtcaaaatgg
     3481 taggtattcg taatatggcg atgcaaagat accgagttga actgggtcaa cactgcaata
     3541 cgatagatcc ccgagttaat acaattgctg atcggaatat caatgaggcg atattttcct
     3601 gcaatcggaa ccgcaggctt ggagcgcatc tgcgtcaaag gatacaagcg tgcgccgcga
     3661 cctccaccca aaatcactgc aagtacttca ttcaacgagg gcatagtagt gtctcctgaa
     3721 gcgtatttta ctgttttttt cgcaacttgc taagttggac tttccctcga ttcatggctg
     3781 tagcaaagtc aaaaaagctc aatgtgaatg acgcgaataa aactatagat cttgacccta
     3841 atgaatggtt ttttttgttt acccgtgaat accagccagc acttttaaag atcacggcat
     3901 gtgtttctgc ctgccaccca gcctcttctt aacatgatga acaacataaa cgatcgggta
     3961 ggccaggagg atcaacaaat ctatcacggc gaagaaaaga taaagtttca tcacacacct
     4021 ccttcataaa atcaaactac acagtacaaa gcataacgta aatgctgtat ataccatttt
     4081 acagttaaaa aacagggtgt caacgcaggc agactcgatg catataaaac tcaaacgtcc
     4141 tcccatgatg ggaggacgtt cgggttataa taccgcttcg ggtatcagcc agtgccgaag
     4201 gagggaatcg aaccctcatt cccggaggga acacgatttt gagtcgtgcg cgtctgccta
     4261 ttccgccact tcggcattcg gttactgagc gcccgatagt ataacaaact ctctcgaata
     4321 aggtcaagat gaaactagga attatcggac tcccccaatc aggaaaaacc accattttca
     4381 acgcgctcac gcgcagcaat acccccacca gcgcatccgc cgggcgtatc gaagttcaca
     4441 ccgccgtcgt ggatgtaccc gacccgcgtg tgaccaaact ctccggcatg ttcaacccga
     4501 agaaaaccat ctacgccaaa gtgacctatg cggatattgc cgggctggaa tctggttcgg
     4561 ccaaaagcgg catttccggg caactgttga atcaactcaa ccaaatggaa ggcctgatcc
     4621 tcatcgtgcg cggattcgcg gacgataacg tcatgcaccc aaatggcagt attgacccgc
     4681 tgcgcgatgt ggacagcatg ttaaccgaac tgctgctcaa cgacatgatc gccgtggaaa
     4741 gaaaacttga aaaattgacc gaagaacgaa aaaagggcgg caccgacaaa gtcatcaatg
     4801 cgcgccagac agagttgttc gaaaaactcc acgcaaccct ttcagataac aaacccctgc
     4861 gcagcatgga atttacgaac gaagagcaaa aagaactcgc cagctatggc ctgctcaccc
     4921 gcaaacccat cctcaccgtg ttcaatatgg gtgaaggaca aaccgcgccc acagcccagc
     4981 tcgatcaccc cagtgtggcc ttgatgggca aactcgaaat ggagatcgcc caactcagcg
     5041 cggaagatgc cgccgttttt atggaagaat atggcatcaa agaattaagc ctcaaccgca
     5101 tgatcaacct ctcctacgac ctgttgaaag tgcagacctt cttcaccgtc ggcgaagatg
     5161 aagtgcgcgc atggacaaca cgccgtggag caagcgccgc agaatcggca gcggaaatcc
     5221 acaccgatct gcagcgcgga tttgttcggg cggaggtggt ttcctacgac gacctgacca
     5281 gtctcgggtc gatgaatgaa gcgaaggcca aagggaagtt aagactcgaa ggcaaggaat
     5341 atccagttaa ggatggtgac attatgcaca tccgatccag tctataatct agttgatacg
     5401 cccttctggt ttttatataa tgagattgcg gagattaagt tccgcaatct catttttttg
     5461 ggagggcacc atgtttagag agcgtggggt atggggggcc atactgctat cgctgatctt
     5521 tctgttcgtt ctggcctgcg gcatatccgt aacgcccaac accagcagcc cgcctgcatt
     5581 tgacccaacc aaggcggcgc ttgaactcca ggctacaagc atgtctctgc aattaaccca
     5641 ggccgcattg aatgcgcaac aagcggccca gtcaacatct cccccaccac aaccaaccgc
     5701 ggcaccaaca gaagcgccag cacaagccca gcccacaccc acacaggatg tggaagcgcg
     5761 catccgcgcc gccaatgtgc tggtttacga aaacacagac gagtacggca tcggcatgtg
     5821 gattcaagat gcgcttgaca acatgggcat caaatacacg cagaccggtt cctacagcgg
     5881 gcacttcatg gagtatctca actccggcac aaaatatgac ctgatcatcg tcggcgcgga
     5941 ggcaaaggat cgcatctccg gcgagttttg ggatgtgatc aacacccgcc tgacccgcga
     6001 caaggtggca ctgatcgctg aggtctggta tctcgacaca gaagcgaatg gaccgatcag
     6061 caaaatactg ggcggctgtg gaattcgcta ccaaaaggat tatcaactgg cggattcgat
     6121 ctactggtgg gactcaacac atcccgtttt caatgaaccg aatacggtgc ttccgcttct
     6181 tcactatgac cgatactggg ccaaccaaac cggagatcag attcgcctcg gcggctccgg
     6241 ggatgccgtg ttgctggcag gcttggctgc gcagaaatcg aacgaggaag ccgtgctagc
     6301 cacctgctat gaaggccgtg tcatcatcca gaccttttcg gatcatgatt acaaaatggc
     6361 agatgtgatt ccgctctggg agaattacat ccactacacg ttgaaaaatc actttttggc
     6421 tgtgccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntcaa aaatccatcc
     6541 cgatgacgta taatttggag gctgtaaatt attttttgga gaagacaaaa tggcttatac
     6601 ccagaaaaaa tggagccttg ctccactgta ccccggttat gaaagcgccg agctgcaaaa
     6661 cgcgtttgac atgatcgaag agcaggtcac ctcttttgag ggcgtgcgcg gaaaactcaa
     6721 ccctgagatt tccgcggaac aattcatgca gatcgtccgt gccagtgaag agacgctgcg
     6781 catcgcaaac aagttatatt cctttgcggg cctgtctttt acagcagata cgcaagacca
     6841 ggcggcgcaa tccctgcaaa accgcgccat gcaatttctg gcagagatcg aaaaccgcac
     6901 catgttcttc aatttatggt ggaaggaagt ggacgagacc aatgccaggc gcctgatgga
     6961 tgccgctggc gattaccgct actatctcga agcgctgaga ttgttcaaac cgcacaccct
     7021 gaccgaagcg gaagagaaga tcgtcaacat caagaatgtg actggctcca acgcgctgat
     7081 caatctctat gatgcgatca ccaatcgcta taccttcaag atgcgtgtgg gcggcaaaga
     7141 aaaggaaatg accgcggcag agcttcaatc cttccgctat agcaccgacc cgaaagtgcg
     7201 cgcggaaagt tatcaggagc agttccgcgt gtatggcaat gacggcgtga tcctcgggca
     7261 aatgtaccag acccgcgcac gtgactggca caacgaaaac attgacatgc gcaagttcac
     7321 cagtccgatc gcggcgcgca acttgaacaa tgacatcccc aacgaagccg tggatgcgct
     7381 gttgaacaca gcccgcaaga atgtaaaaat cttccagcgt tatttcaacc tgaaagccaa
     7441 acatgtcggc atgaaaaaac tgcgccgcta tgacatttac gcgcccgtgg cacgctcaaa
     7501 gaagacttac gaattcgata ccgccgcaga catggtattg gaggcattct ctgcattcga
     7561 cccgaaagtg gcggaattgg ccaaacgtgt ctttgatgaa gaccgcattg acagcgaaat
     7621 tcgcaagggc aagcgcggcg gcgcgttctg ttggtctgtc ctgccggaga tgactccgtg
     7681 ggtgctggtc aactatcagg ggacgggacg cgaggttgcg actctggcgc atgaactcgg
     7741 ccacgccatc cattccatgc tggcggcggg gcacaacacc ttttcgttcc actcctctct
     7801 cccgttggca gagaccgcat ccacatttgc ggagatgatg ctgatcgaca agatgctggc
     7861 tgaagagacc gatgaatccg ttcggcgcga catcctcttc aaacaaatgg acgatgccta
     7921 cgccaccatt ttgcgccagt catattttgc gctgttcgaa aagcaggcac atgaaatggt
     7981 gcagaagaac gcctccgtgg atgagttgtg cgcagcctac atggaaaacc tgaaagagca
     8041 attcggcgat tcggtggagt tgagcgacga attcaagtgg gaatgggtcg gcattccgca
     8101 catttaccac accccgttct acgtgtatgc ctatgccttc ggacaattgc ttgtgctttc
     8161 gctgtatcag cagttcaaag cggaaggcga atcgttcaag ccgaaatatc tgaaaattct
     8221 ttcggcgggc ggatcagaag cgcccgaaaa aatcctcacc gaagcaggcg tggacatccg
     8281 ctcggcgaag ttctggcagg gcggtttcga tgtactcgaa aaactggtga gcgagttgga
     8341 gcagttgcca gcggataagc cgaaggtgaa aaaggctgt
//
LOCUS       DAOQLC010000155         8362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3409_length_8362_cov_24.659685, whole genome shotgun sequence.
ACCESSION   DAOQLC010000155 DAOQLC010000000
VERSION     DAOQLC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3409_length_8362_cov_24.659685"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PL000_18695"
     CDS             complement(<1..796)
                     /locus_tag="PL000_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNC90967.1"
                     /translation="MAKTLFDKIWDSHVVTEQEGAPAVLYIDLHLVHEVTSPQAFTGL
                     RQRGLKVRRPDKTLATMDHSIPTTPIDVPIADAMAAAQIKQMETNAAEFGITLHGMTS
                     PHRGIVHVIGPELGRTQPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLLQ
                     KKPKTYEVRVDGKLGPGVSAKDIILALIAKIGVGGGTGHVFEYTGEAIRGLTMEQRMT
                     ICNMSIEGGARAGMIAPDEVTFEYLKGREFAPQGADWDKAVAYWKTL"
     gene            complement(917..2551)
                     /locus_tag="PL000_18700"
     CDS             complement(917..2551)
                     /locus_tag="PL000_18700"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC90968.1"
                     /translation="MTNYVKIFDTTLRDGEQSPGATMTSAEKLEVAHNLARLGVDIIE
                     AGFPAASPDDLEAVRRIALEVGNPAHPESDAKIPVIAGLARANKTDIDKAWEAVQGAK
                     KPRIHTFLATSAIHMKHKLKMDPEEVVQRVSEMVAYAKSLCADVEFSPEDAGRSDPEF
                     LYVVLGEAIKSGATTLNIPDTVGYTTPDEFFKLIDGIIKNTPGMHEGITVSVHCHDDL
                     GLATANTLAGIRAGARQAEVTINGIGERAGNTSLEEVVMALKTRHPIFGLETGIETPQ
                     LSRISKLVSNYTGMIVQPNKAIVGANAFAHEAGIHQDGMLKHQTTYEIMRPEDVGVNQ
                     TKLVLGKHSGRHALRNRLAEMGHSLDEVELDKAFARFKELADRKKVITDLDLEALIAD
                     EFYKPRDVYLLNGLQVTSGTMGMPTATIRLRGPDGIVHTIAAIGTGPVDATYKAIDAI
                     VNVPCTLLEFNIHAITEGIDALGEVTVRIQSDSSNTTMDPQSEVEYIRVYGGHGADSD
                     IIVASAKAYINALNKLIVAQTEGKGKVPNDFGVMLG"
     gene            complement(2629..3684)
                     /gene="ilvC"
                     /locus_tag="PL000_18705"
     CDS             complement(2629..3684)
                     /gene="ilvC"
                     /locus_tag="PL000_18705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNC90969.1"
                     /translation="MAKINFGGVEETVVTRDEFPLEKAREVLKNEVVAVIGYGVQGPA
                     QAMNMRDNGINVIVGQREGTPNWDKAVADGWVPGKTLFSVEEAAEKGTVIQYLLSDAG
                     QRSLWPKIVECLNEGDMLYFSHGFSVTFKDDTGVVPPPNVDVALVAPKGSGTSVRRNF
                     LAGSGINASYAVHQNFTGKAEERTIALGIAIGAGYLFQTDFRNEVYSDLTGERGILIG
                     ALSGVMEAQYNVLRKHGHSPSEAFNETVEELTQSLIRLVGENGMDFMYANCSTTAQRG
                     ALDWHPRFRDAVAPVFDALYESVISGNEARITLEANSRADYREQLEKELAAFRDSEMW
                     RAGAAVRSLRPENWKKS"
     gene            complement(3784..4344)
                     /gene="ilvN"
                     /locus_tag="PL000_18710"
     CDS             complement(3784..4344)
                     /gene="ilvN"
                     /locus_tag="PL000_18710"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNC90970.1"
                     /translation="MNYTFIALVENRPGVLNRVASLFRRRNFNIESLAVGRTEDPEVS
                     RMTVVVDCPNGDIDAHKIEANLYKLVNVIDVQDVTRQPSVTRDLALIKVKVGPEKRAE
                     VNGLADIFRARIVDVASDSVIVEITGTEDKIEGMIELLRPLGIVEMVRTGQVAMTRGV
                     NDGVRRVPGVTGHRFEDVSEMMEMTP"
     gene            complement(4398..6083)
                     /gene="ilvB"
                     /locus_tag="PL000_18715"
     CDS             complement(4398..6083)
                     /gene="ilvB"
                     /locus_tag="PL000_18715"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNC90971.1"
                     /translation="MKKTGAEIMWECLVREGVEVVFGYPGGANMPIYDAMLSYPVHHV
                     LVRHEQGGAHMADGYARASGKVGVAMATSGPGATNLVTGIATAMMDSVPVVFITGQVA
                     AHLIGGDAFQETDVTGITLPITKHNYIVTRADEIAEVVREAFYIARSGRPGPVLIDIC
                     KNAQIETCEFEYPEEVKLPGYQPVDHAPKASLSEAVKLIEKARKPIILCGHGVLMSKA
                     ENELMQFAVKTQTPVASTLLGLGAFPASHELSLGMMGMHGEVHTNLAIQNSDLILAFG
                     MRFDDRVTGTLKTYAPRAKKIHIEIDPSEVHKNVAVDVPLVGDLKTVLSDLIPLVDEY
                     DHEDWKQEINGWKSEADKRSIMNWDADGKLYVAHLISDIWKATGGGAIVTTDVGQHQM
                     WTAQYYQLDKPNRWISSGGAGTMGFGLPSAIGAWFAAKDQEIWAIAGDGGFQMTAAEL
                     TTAVQEGANVKVAIMNNNFLGMVRQWQEFFFEKRYSAVNMLTPDFVKLADAHGVPARR
                     VTKREEVADAIEWARKTKGPVVLEFKVEREDAVYPMVPAGNALDQMLQRPIKQ"
     gene            complement(6142..7821)
                     /gene="ilvD"
                     /locus_tag="PL000_18720"
     CDS             complement(6142..7821)
                     /gene="ilvD"
                     /locus_tag="PL000_18720"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC90972.1"
                     /translation="MRSDTVKKGYEKAPHRALLRATGLKDEDFNKPFVAIVNSYVDVV
                     PGHVHLQEFGKMVKDAVRAAGGVPFEFNTIGVDDGIAMGHMGMKYSLPSRELIADCVE
                     TMVEAHRFDAMVCIPNCDKIVPGMLLAAMRVNVPTIFVSGGAMAAGKTPDGETLDLIS
                     VFEGVGAFSAGKIDEKRLSILEKFACPSCGSCSGMFTANSMNCLMEVLGLALPYNGSA
                     LAKTPEREALARQAAAQVMTLIERDIKPRDIVTAEAIDDAFALDMAMGGSSNTVLHTL
                     ALANEAGVDYPLTRINAVADKVPHICKVSPAGKWHMEDVHRAGGIPAILNEVQRGTGM
                     LHLDRMTVTGKTLGESIQGCEIKDEEVIRRYENAHSKRGGLSILFGNLAPNGAVVKVG
                     GVSEAMMKFEGPAVIFESQDEAMAGILAGKVKAGDCVVVRYEGPKGGPGMQEMLSPTS
                     AIMGQGLGDKVALITDGRFSGGTRGACIGHVSPEAAAGGPIGALKAGDIVQIDLVARR
                     LDVKLSEAEIQSRLAALPPFQSKITSKWLKRYSYFVTSADTGAVLSTDGGR"
BASE COUNT         1889 a         2367 c         2062 g         2044 t
ORIGIN      
        1 gcagggtctt ccagtaggcg acggctttat cccagtcggc gccttgggga gcgaattcgc
       61 gtcctttgag atattcaaag gtgacctcgt caggggcgat catacctgca cgcgcgccgc
      121 cttcaatgga catgttgcag atggtcatgc gctgttccat tgtgagtcca cgaatggctt
      181 cgccggtgta ttcgaagaca tgcccagttc ccccacccac gccgatcttg gcgatgagcg
      241 cgaggatgat gtctttggca gaaacaccgg gtccgagttt gccatccacg cggacttcgt
      301 aggtcttggg tttcttctgc aagaggcact gggtcgcaag aacatgctca acctcagacg
      361 tgccaattcc gaacgcgagt gcgccaaatg cgccatgtgt ggccgtgtga ctgtcaccgc
      421 agacgatggt catgccgggc tgggtgcgcc cgagttccgg tccgatcacg tggacgatgc
      481 cgcggtgcgg actggtcatg ccgtgcaggg tgatgccgaa ttcagcggca ttggtttcca
      541 tctgtttgat ctgcgcggca gccattgcat ctgcaatggg aacgtcaatc ggtgtggtcg
      601 ggatggaatg atccatcgtc gccagcgtct tatcggggcg gcggactttg agtccgcgtt
      661 ggcgcaaacc tgtaaaggct tgaggagatg ttacttcgtg gaccagatga aggtcaatgt
      721 aaagaaccgc aggggcaccc tcctgttcag tcacgacatg agagtcccag attttatcga
      781 aaagagtttt agccataaag tttccttgta atatgtcatt gcgaggaacg cttttgccct
      841 tcgcgacgaa gcaatctcct aataattcaa ggattgcttc gggcgaaaga gcaccgccct
      901 cgcaatgacg tgatacttat cccagcatca cgccaaaatc attcggcact ttccctttgc
      961 cttctgtttg ggcgacaata agtttattca acgcgttgat atatgccttg gcactggcaa
     1021 cgataatatc tgaatcggcg ccatgtccgc catatacacg gatgtattcc acttcgcttt
     1081 gcggatccat ggtggtgttg ctgctatcgc tttgaatacg gacagtcacc tcacccagcg
     1141 catcgatccc ttcggtaatg gcgtggatgt tgaactccaa caaagtacag ggcacattca
     1201 cgatcgcatc aatggcttta taggtcgcat ccaccgggcc tgtaccgatc gcagcaatag
     1261 tgtggacaat gccatctggc ccgcgtaaac gaatggtagc ggtgggcatg cccatggtgc
     1321 cgctggtaac ctgcaacccg ttgagcaaat acacatcacg cggtttgtag aattcatctg
     1381 caatgagggc ttcgaggtca aggtctgtaa tgaccttctt acggtcggcc agttccttga
     1441 agcgcgcaaa tgccttgtcc agttcaactt cgtcaagcga gtgccccatt tcagcgaggc
     1501 gattccgcaa cgcatggcgg ccggagtgct tccccaacac cagcttggtc tgatttacgc
     1561 caacatcttc agggcgcatg atctcataag tggtctggtg tttgagcatt ccatcctgat
     1621 ggatccctgc ttcgtgggca aatgcattcg cgccaacaat ggccttgttc ggctggacga
     1681 tcatgcccgt gtaattgctg accagtttgc tgatgcgtga gagttgcgga gtctcaattc
     1741 ccgtctcaag gccaaagatg ggatgacgtg tcttcaaggc catgaccact tcttcgagcg
     1801 aggtgttccc tgcacgttca ccgatgccat tgatggtcac ctccgcctga cgtgcccccg
     1861 cacgaatgcc agccagggtg ttggcggtag caagtccaag gtcatcatgg caatgcacag
     1921 agactgtgat gccctcgtgc attcccggtg tgttcttgat gatgccatcg atcaacttga
     1981 agaactcatc gggcgtggta tatcccactg tatcgggaat gttcaatgta gtggcgcccg
     2041 acttgatggc ttcacccaaa accacataca aaaactcagg gtcggagcgg cccgcatctt
     2101 cagggctgaa ctccacatcg gcgcacagcg acttggcgta tgccaccatt tccgaaaccc
     2161 gttgcacgac ctcttcggga tccattttca acttatgctt catatggatc gcagatgtgg
     2221 caagaaaggt gtggatgcgc ggcttctttg cgccttgcac agcctcccag gccttgtcga
     2281 tatcggtctt gttggcgcgt gccagcccgg cgatgacagg aattttcgca tcagactcag
     2341 ggtgggctgg gttgcccact tcgagagcaa ttcgtctgac ggcttccaga tcatcgggcg
     2401 aggcggcggg aaatcccgct tcaataatat ccacgccgag acgtgcgaga ttatgagcca
     2461 cctccaactt ctccgcagag gtcatcgttg ccccgggaga ttgttctccg tctcttaaag
     2521 tcgtgtcgaa tattttcaca tagttggtca taggtattcc tttgggtaat tagagatcag
     2581 taattggaga ttatgttatt ccaatctcta attaccaatt accaaaaact aactcttctt
     2641 ccaattttca gggcgtaatg aacgtaccgc cgctccagcg cgccacattt cagaatcacg
     2701 gaaagcagca agttcctttt caagttgttc gcgataatct gcgcggctgt tggcctcaag
     2761 agtgatgcgg gcttcgttgc cgctgatcac agattcatac aatgcatcga acacgggggc
     2821 caccgcatcg cggaagcggg gatgccagtc gagtgctccg cgttgggcgg tggtggaaca
     2881 gttggcatac ataaagtcca tgccgttctc ccccaccaga cggatcaggg attgggtgag
     2941 ttcttccacg gtctcattga aggcttcgct gggggaatgt ccatgcttgc ggaggacgtt
     3001 gtactgcgct tccatgactc cggagagggc gccgatcaaa atgccgcgtt caccggtcaa
     3061 gtcactgtac acttcattcc tgaaatcggt ctggaagagg taccctgcac cgatggcgat
     3121 gcccaatgca atggtgcgtt cctcggcttt accggtgaaa ttttgatgca cagcatagct
     3181 ggcattgata ccgctcccag caaggaagtt tcggcggaca ctggtgcccg aaccctttgg
     3241 agccaccaaa gccacatcca cattgggagg aggcacaacc ccggtatcat ccttgaaggt
     3301 cacggaaaaa ccgtgcgaga aatacagcat atcgccttca ttcaaacatt ccacgatctt
     3361 gggccacaaa gagcgctgcc ctgcatcaga gagcagatat tgaatgaccg ttcctttttc
     3421 cgcggcttct tccacggaga aaagggtttt gccggggacc caaccatctg caacagcctt
     3481 gtcccaattc ggcgtgcctt cacgctgacc gacaataacg ttgatgccgt tgtcgcgcat
     3541 gttcatcgcc tgcgccgggc cctgtacacc gtacccgatc actgccacta cttcattctt
     3601 caagacctca cgcgcttttt caagcggaaa ttcgtcgcga gtcacaaccg tctcttccac
     3661 gccaccaaaa ttgatttttg ccatgattac tattctcctt aagatttttt ttagttagtt
     3721 acaggtttca agtttcaggt ttcaggcttt attcatcctt catccttcaa aattcatcat
     3781 ttcttacggt gtcatttcca tcatttcaga aacgtcttcg aagcgatgac cggttacacc
     3841 cggcacgcgg cgcacgccat cgttcacgcc gcgggtcatg gccacctgcc cggtgcggac
     3901 catttccaca atgcccagcg ggcgcagcaa ttcgatcatg ccttcgattt tgtcttcggt
     3961 gccggtgatt tccacgatga ccgaatccga ggccacatcc acgatgcggg cgcggaaaat
     4021 atcggcgagg ccgttgacct cggcgcgttt ctcggggccg acctttacct tgatcagagc
     4081 caggtcacgg gtcaccgaag gttgacgcgt gacgtcctgc acatcaatga cattgacgag
     4141 cttgtaaaga ttcgcttcga tcttgtgcgc gtcgatatcg ccattggggc aatccacaac
     4201 cacggtcatg cgcgaaacct cggggtcttc cgtgcgtccc acagccagcg actcgatatt
     4261 gaaattgcgg cgtcggaata acgatgcaac gcgattcaat acgccgggtc tgttttcaac
     4321 taatgcaatg aaggtgtagt tcatatgtct ctccagtaat ctccagtcag caatatgtgg
     4381 agatcggtat ttggtaatta ttgttttatc ggcctttgta acatctgatc cagcgcattg
     4441 cctgcaggga ccattggata cacggcatcc tcacgctcga ccttgaactc gagcaccacg
     4501 gggcctttgg tcttgcgcgc ccattcgatg gcatctgcca cctcttcgcg tttggtcacg
     4561 cggcgggcgg ggacgccatg tgcgtctgca agtttcacga aatcaggcgt aagcatgttt
     4621 acggctgagt agcgtttttc aaagaagaat tcctgccatt gacgcaccat gccaaggaag
     4681 ttgttgttca tgatggcgac cttcacattt gcgccctcct gcacagcagt ggtgagctct
     4741 gccgcggtca tttggaaacc gccatccccc gcaatggccc agatctcttg atcttttgca
     4801 gcaaaccatg caccaatggc agagggcagg ccaaagccca tggtgccagc gccgcctgaa
     4861 gagatccagc ggttcggctt gtccaattgg taatattgcg cggtccacat ttggtgctga
     4921 ccaacatccg tggtgacgat cgcgccacca cccgtggcct tccaaatatc agagatgaga
     4981 tgcgccacgt acaattttcc atccgcatcc caattcatga tgctgcgttt atcggcttca
     5041 gatttccagc cgttaatttc ctgcttccaa tcttcgtggt cgtattcatc caccagcgga
     5101 atgagatcgc tcaaaacggt tttgaggtcg cccaccaggg gaacatccac tgctacgttc
     5161 ttatgcactt ccgaaggatc gatctcaatg tggatcttct tcgcacgcgg cgcataggtc
     5221 ttgagcgtgc ctgtcacacg gtcatcgaaa cgcataccaa aggcgaggat caaatcggaa
     5281 ttttggatcg ccaggttggt atggacttcg ccatgcatcc ccatcatgcc gaggctcaac
     5341 tcatgcgaag cagggaaggc acccaaccca agcagggtgc tggcaaccgg ggtttgcgtc
     5401 ttcactgcaa attgcatcaa ttcattttca gccttcgaca tcaaaacacc atgtccgcac
     5461 aaaatgatcg gcttgcgggc tttctcgatc aatttcacag cctcgctcaa cgaagccttg
     5521 ggagcatggt ccacgggttg atagccgggc agtttcactt cttccggata ttcgaactcg
     5581 caggtttcga tctgggcatt cttacaaata tcgatcaaca cggggccggg gcgtccgctg
     5641 cgggcaatgt aaaaagcctc gcgcaccact tccgcgatct catcggcacg ggtcacaatg
     5701 taattgtgtt tcgtgatcgg aagtgtaatg cccgtcacat ccgtttcctg aaatgcatcg
     5761 ccgccgatca aatgcgcggc cacctggcca gtgatgaaca ccacagggac cgaatccatc
     5821 atggcggtgg caatacccgt cacaagattc gttgcaccag gacccgatgt tgccatggcc
     5881 accccaacct tacccgaagc gcgtgcatat ccatctgcca tgtgtgcgcc gccctgttca
     5941 tgacgcacca acacatgatg caccggataa ctaagcatcg catcgtagat cggcatgttc
     6001 gccccgcccg gatacccgaa gaccacttcc acgccttcgc gcaccaaaca ctcccacata
     6061 atttctgcac ctgtcttttt cattctcttc tccttgattt agaccagaga ccgttgagca
     6121 caaaccatga tggcctgttg cctaccgccc accgtctgta gataacactg cgcctgtatc
     6181 tgcgctggtg acgaaatacg aatatcgctt taaccacttt gaagtgattt tggattggaa
     6241 tggaggcagc gccgccaagc gactctggat ctccgcttcg ctcaacttaa catccagcct
     6301 gcgtgccacc aaatcaattt ggacaatgtc cccggctttt agcgccccaa ttggaccacc
     6361 cgcagcagct tccggcgaca cgtgaccaat acatgcgcct cgcgttccac cactgaagcg
     6421 gccatcggta atgagtgcca ccttgtcgcc aagtccctgc cccatgatcg cagaggtggg
     6481 cgaaagcatt tcctgcatac cgggtccacc ctttggccct tcataacgga ccaccacaca
     6541 atcgccagcc ttgaccttgc ccgccaaaat tccagccatg gcctcatcct gcgattcaaa
     6601 aataacggca gggccttcaa acttcatcat ggcttcactt acgccaccca ctttgaccac
     6661 tgccccattc ggcgccaggt tgccaaacaa aatggataaa ccaccacgtt tggagtgggc
     6721 attttcatac ctgcgaatca cttcttcatc tttgatctca caaccttgaa tcgattcccc
     6781 cagtgtcttt cctgtgaccg tcatgcggtc caaatgcagc atacccgttc cccgttgcac
     6841 ctcgttcaaa atggcaggga ttccccccgc gcgatggaca tcttccatat gccatttgcc
     6901 cgccgggctg actttgcaga tgtgaggcac cttgtctgcc acggcattga tgcgggtcag
     6961 cggataatcc acacctgctt cattagccag agccagcgta tgcagcaccg tgttggaaga
     7021 cccacccatc gccatatcca aggcaaacgc atcgtcaatg gcttcagcag tgacaatgtc
     7081 tcgcggcttg atatcgcgtt cgatcagggt catgacctgc gcagcggcct gccttgccaa
     7141 tgcttcgcgt tcgggggtct tcgccagtgc agatccgttg tacggcagag ccaatccgag
     7201 cacttccatc aaacagttca tggaatttgc ggtgaacatg cccgagcatg aaccgcagga
     7261 tgggcaggca aatttttcca aaatggataa acgcttttcg tcaattttcc ccgctgaaaa
     7321 cgcacccacc ccttcaaaaa ctgagatcag gtcaagggtt tcgccatcgg gtgtcttgcc
     7381 tgcagccatc gcaccaccag agacaaaaat ggtcggcacg ttcacgcgca tggcggccaa
     7441 cagcatgcct ggcacgatct tgtcacagtt tggaatacag accattgcat cgaatcggtg
     7501 ggcttcgacc atcgtctcca cacaatctgc gatcagttcg cgggaaggaa gtgaatactt
     7561 catccccata tgccccatgg caattccatc gtccacgcca atggtattga attcaaaagg
     7621 cacaccgccc gccgcacgga cagcatcctt caccattttt ccgaattcct gcaaatgcac
     7681 gtgcccgggg accacatcta cataagaatt aacgatggca acaaaaggct tattaaagtc
     7741 ttcgtctttc aaacccgtgg cacgcaacaa tgcacggtgt ggtgctttct catacccttt
     7801 tttaactgta tctgatcgca taatgctcct ttgatgtgta gtgcttgacc gtggactgcg
     7861 gtcaatcttc tgtagcctgt tttttataca aaaagagccg cccgtggtta gggcggctct
     7921 tcgtacttac ttagtgattt actttactct caccgtagcc atcctaaccg aaaacagatc
     7981 cttaataata aggaccgcaa ggactactac aataagggag agggtgaaca aagatgtgct
     8041 catgggttgt gacttcgccc gattataggc agattcggaa ataagtcaag gggggttgtg
     8101 tcggattcgg ggatgctaat cgtttacatt ttgtagaatc atcaacgttc ctgaataggg
     8161 catctattca ccttcgctag gaaaacgccc tgctgaaaca cctaaatttg gtatttggtc
     8221 tgtagagttg gtccaatcat ttgcgctgag ttggcaagtc ttgcaacaca acaacatacc
     8281 agcaaaaaag cacttacatt ttccccttgg aagtataatg accatatatg tccatccttt
     8341 ccgataaaca catcctgctt gg
//
LOCUS       DAOQLC010000156         8067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3594_length_8067_cov_19.939216, whole genome shotgun sequence.
ACCESSION   DAOQLC010000156 DAOQLC010000000
VERSION     DAOQLC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_8067_cov_19.939216"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2243)
                     /gene="recJ"
                     /locus_tag="PL000_18725"
     CDS             complement(<1..2243)
                     /gene="recJ"
                     /locus_tag="PL000_18725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNC90973.1"
                     /translation="MTSRWLDPQPVNTDPLHALGLPPLVAQTLLRRGISSPVEAEAFL
                     HPDATPPVSFPDIEKAVAAILVAIRKNDLICVWGDFDVDGQTSTALLVQTLQILKANV
                     TYYIPIRGRESHGVHIESLKPILDHGAKLLLTCDTGIAAHEAIEYANSRGVDVVVTDH
                     HDLGETLPNAKAIINPKLLPEDHPLKHLAGVGVAYKLAEALLAENHPSELANLLDLVA
                     LGLIADVALLKGETRALAQKGIEALRATNRLGLKVIAELSGTALETLTEETIGFTFAP
                     RLNALGRLGDANPAVELLLTKDPPRARVLSAQIEGLNAQRRLLTSQVYEAAEAKLREA
                     PDLLTEPAIVLSHPGWPGGVVGIVANRLVDRYHKPALLLTESEDGIVRGSARSVEGLH
                     ITEAIATQKDLLLGFGGHPMAAGLSMPLDKLADFRRGLGQAVEKQLGEIVFDEPTLQI
                     DAWLDLAEINISLADALEMLAPFGAGNPALTLATRGVTLKSVTTIGKTKEHLRLTIED
                     EAGKTQSILWWGGAGEELPPTDNKFDIAYSLRASTFRGQKQVTLQFEEFRVVEEKPIE
                     ARKTKVEIRDMRLQSSLLNLQPSTLIWAEGADRAVGTPRFELRPADEFSIYTTPASPA
                     DLRKALGIVKPKIVYVFAIPPAEEKPEEFLTHLAGLCKFTLNQRRGKVTVRELAAAMA
                     TRESAIQIGLEWMSAGGQLSVAFDEDQITLSAEKQEKNPYLQAELFVALKGILNETAA
                     YRKYFASA"
     gene            2490..3086
                     /locus_tag="PL000_18730"
     CDS             2490..3086
                     /locus_tag="PL000_18730"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC90974.1"
                     /translation="MKIAIIGAGNIGGTLGRKWARTGHEVRFGVRNPADSKFASLRSA
                     GAVVSVAEALVAADVVLLSMPGGAVADFAEQHGATLAGKIVIDSTNSVRNAHMNNLAA
                     LKEKSPEAKLIRAFNALGWENFEVPQINGVQIDLFYCGHAESRTITDQLIAEIGLRPI
                     YIGDLDLAAVLDGMTRMWFALALAQGRGRRIAFKLMEE"
     gene            3086..3499
                     /locus_tag="PL000_18735"
     CDS             3086..3499
                     /locus_tag="PL000_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HNC90975.1"
                     /translation="MASVGYSGTPLAKKLGLKPTLTLLTINAPKEYRSWLGELSGGVS
                     LVETAREPYEAVHVFATESAFLDGILSKLRRELKQDGFVWVSWPKKSSKVPTDITEDV
                     IREIALPLGFVDIKVCAVSEVWSGLKLVIRKSERS"
     gene            complement(3513..4448)
                     /locus_tag="PL000_18740"
     CDS             complement(3513..4448)
                     /locus_tag="PL000_18740"
                     /inference="COORDINATES: protein motif:HMM:NF024926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance efflux transporter family
                     protein"
                     /protein_id="HNC90976.1"
                     /translation="MTRLIAIGSLAALFFSSTFVLNRAMSLQGGHWAWTSSLRFGFML
                     IFLILLLLATQGKKALAEVRRVFLSHWLFWILAGSIGFGVFYSLITFSSQYAAGWVVA
                     TTWQTTILATPIVLVFFGRKVPTKGLLFTGIIFVGIILVNIEHAALTSLREVLFSALP
                     VLIAAFAYPIGNQLVWEARMGHNARIPKIENPILENGFARVLLLTLGSIPFWVLILLF
                     SSPPAPASGQLLSTALVALLSGVIATTLFLYARHLCHQPYEIAAVDATQSMEVVFSLI
                     GEIVFLHGAFPGPIGFVGVALTILGLIAYMKVQAN"
     gene            complement(4449..5261)
                     /locus_tag="PL000_18745"
     CDS             complement(4449..5261)
                     /locus_tag="PL000_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90977.1"
                     /translation="MLSNLSRWLTYTCALLYAILGVLLFLFPSQLAPVFAWKVTAFMT
                     ITIGGWCLGNAWLAYINARRWEWKLVYSSLVYLWLFGIGELIVLFVFRDKLKLEHPIA
                     WLYFITLIVNALAAFVGVFDYLRIRPSNQSNGPTFTKIQSLPAFAFVLFVGFLGLYGL
                     TAQIGDIGTNAGIFPEVMSLFTLRSFGVFYFALALGVVPYFWDRNLNAILHHSFAAYP
                     LVVFITTAAFIYIRLFDFAKRPGGLAYFGVYLAVGIPLIFVFIKYGTGGSRN"
     gene            complement(5441..6823)
                     /locus_tag="PL000_18750"
     CDS             complement(5441..6823)
                     /locus_tag="PL000_18750"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNC90978.1"
                     /translation="MTPKFYPPEPYRIKVTEPIRLISPEAREIALKEAGYNLFSMKAE
                     DIFIDLLTDSGTGAMSQEQWAAIMRGDESYAGARSYQRLKTAVEDIFGFKFFVPTHQG
                     RAAENILSACLVKPGNYIPSNMHFDTTDANIRARGGRPTNLVIDEAFDPANPHPFKGN
                     MDINKLRAFIQKVGVANIPFGMVTVTNNAGGGQPVSMENLKAVAAVYREFNIPFFIDS
                     ARYAENAYFIKLREKGYENKSVIDIAREMYALADGMTMSAKKDAIVNIGGLLCLNDET
                     LFGAIRNELILREGFPTYGGLAGRDLDAMAVGLYEGLDEAYLASRLTQTAYLAARLNE
                     AGIPTIQPAGGHAVYLDAAHVFPNIPQSEFPGQALAVELYREGAIRGVEIGSVMFAYP
                     DPDSGKMIHPKLELLRLALPRRTYTQSHMDYVADCAAQIKSRADKVRGYKFTFAPELL
                     RHFTARFEPL"
     gene            complement(7078..7656)
                     /locus_tag="PL000_18755"
     CDS             complement(7078..7656)
                     /locus_tag="PL000_18755"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC90979.1"
                     /translation="MEAYSESLELFRSISPTSVSVSVTLTHIATAKEANKDYNSAEGD
                     YREALQIAKISDRQDGIAAITGNLAGLAFAREQWAEAESLAREALALAEKVGQQELIA
                     SDCHLLAKALFKQNPVDAVGATRPVQKSALSGNVEAQENDLSDREGSSLHEALLLSRR
                     AVEIYTRLRHPVLQSAQEMLAEIERALQKSER"
     gene            complement(7676..>8067)
                     /locus_tag="PL000_18760"
     CDS             complement(7676..>8067)
                     /locus_tag="PL000_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90980.1"
                     /translation="QYGGGSKDYEKFPMLDTEWDFISAALPRLLTGDNNRLQKVCNSL
                     DKFLDFTGKWDDLLWLNEESEERAIAAGDRETAGLRAYLAGWAYSLRNQSNKVLACAN
                     RAADYWSDSTPGKKANAIKLRGGRPST"
BASE COUNT         1843 a         2050 c         2285 g         1889 t
ORIGIN      
        1 gcgcttgcaa aatattttcg gtaagcggcg gtttcgttga gaatgccttt cagtgccacg
       61 aacaattccg cctgcaggta tggatttttc tcctgctttt cagcagacag ggtgatctgg
      121 tcttcgtcaa acgccaccga gagttgacct cccgccgaca tccactccaa cccgatctgg
      181 atcgcgcttt cgcgggtcgc cattgctgcg gccagttcgc gcacggtcac cttgccgcgg
      241 cgctggttga gcgtgaattt acacaggcct gcaaggtggg ttagaaattc ctctggcttt
      301 tcttcggcgg gcggaatggc aaacacgtaa acgatctttg gtttgacaat gcccaatgct
      361 ttgcgcaaat cagcggggga ggcgggtgtg gtgtagatcg agaattcatc ggcggggcgc
      421 agctcaaagc gtggagtgcc aacagccctg tcggcgcctt cggcccagat gagggtggat
      481 ggctgcaaat taagcaggga tgactgcagg cgcatgtctc tgatctcgac cttggttttt
      541 cgcgcctcga tgggtttttc ttccaccaca cgaaactctt cgaactgtaa cgtgacctgc
      601 ttctgcccgc ggaatgtgga ggcacgcagg gaataggcaa tgtcaaattt attgtcggtg
      661 ggtggcagtt cttcgcctgc gccgccccac cacaaaatgc tttgtgtttt gcctgcctca
      721 tcttcgatcg tcaggcgcag atgttctttg gtctttccga tggtggtgac agatttcaag
      781 gttacgccgc gggttgccaa agtgagcgcg ggattccccg caccgaaggg ggcgagcatt
      841 tccaatgcgt cggcgaggga aatgttgatt tccgccagat caagccaggc gtcgatctga
      901 agcgtgggtt catcgaagac gatctctccc agttgtttct ccaccgcctg accaagtcca
      961 cgtcgaaagt ctgcaagttt atccaatggc atggaaaggc ccgctgccat tgggtgcccg
     1021 ccgaaaccga gcagcaggtc tttttgagtg gcgatggctt cggtgatgtg cagaccttcc
     1081 accgagcgag cggagccgcg cacgatgccg tcttctgatt cggtcagaag caatgcgggt
     1141 ttgtgataac ggtctacaag ccgattggct acaatgccca ccacaccacc aggccagcca
     1201 gggtgagaga gcacaatggc tggctcggtc aacaggtcgg gagcctcgcg caattttgct
     1261 tccgccgctt catacacctg actcgtcaac agccgccgct gtgcgttgag gccttcgatc
     1321 tgtgcagata gcacacgtgc gcgcggcgga tctttcgtga gcagcaattc aactgcaggg
     1381 ttggcatcgc ccaaacgccc cagggcattc aagcgcggag caaatgtaaa gccaatggtt
     1441 tcctcggtca gcgtttcgag tgcggtgcct gaaagttctg caatgacctt caaccccaac
     1501 cgattggttg cccgcagtgc ttcgatccct ttttgcgcaa gcgcgcgtgt ttcaccctta
     1561 agcagtgcca catcggcgat gaggccgagc gccacgaggt cgagaagatt cgcaagttca
     1621 gagggatgat tttcagcaag cagggcttca gccagtttgt atgccacgcc cacacctgca
     1681 agatgtttca acggatgatc ctctggcagc agtttcgggt tgatgatggc ctttgcattt
     1741 ggaagcgttt cgccgaggtc atgatgatcg gtcacaacca catccacgcc gcgtgaattg
     1801 gcatactcaa tggcttcgtg tgcggcaatg cccgtatcgc aagtgagcag caacttcgcg
     1861 ccatgatcga gaatgggctt gaggctttcg atgtgaacgc cgtggctttc cctgccgcgg
     1921 atcggaatgt agtaggtcac attcgctttc aaaatttgca gggtttgcac cagtagggca
     1981 gtggaggttt gcccatccac atcgaaatca ccccacacac aaatcaaatc attttttcgt
     2041 atagccacaa gaattgcggc gactgctttt tcgatatctg gaaaagatac aggtggggtt
     2101 gcatcgggat gaagaaacgc ttctgcttct acgggagagc tgatcccgcg tcgaagcaga
     2161 gtctgcgcga caagcggggg caggcccaac gcatggagtg gatcggtgtt gacaggttga
     2221 gggtcaagcc agcgggatgt catgatgaat tgattttatc gcttgtcatg attttgtgtg
     2281 cgcctttgta cctcaacgtt cacgttgcgt acgtatcctt cgcgcctcgc aaatcgccgc
     2341 cgaggacggc ggctggagcc tgtatgtgag tttcctccac catcctcgac aaaggattct
     2401 ctcgcaggcc cccttacttg tggcgcatat cagatttttc agcatgcgtt atcattgatg
     2461 cagctctttt atctggagag gaactgaaaa tgaaaattgc aatcattggc gcggggaaca
     2521 ttggcggcac gcttggaagg aagtgggcac ggactgggca tgaggtccgc tttggggtgc
     2581 ggaaccccgc tgactcaaaa tttgcctccc tgcggtctgc gggagcggtg gtgtctgtgg
     2641 cggaagcatt ggttgcggcg gatgttgtcc tgctttccat gcctggcggt gcggtggcag
     2701 attttgccga gcagcacggc gcaacccttg cagggaaaat tgtgatcgac tcgaccaaca
     2761 gcgtccgcaa tgcgcacatg aacaatctgg ccgccttgaa agagaagtca cctgaagcaa
     2821 aattgattcg ggcgttcaac gccctggggt gggaaaattt tgaagtgccg caaatcaacg
     2881 gcgtgcaaat tgacttgttc tactgcggcc atgcagagtc gcggacgatc accgatcaat
     2941 tgatcgcaga gattggcctg cgcccgatct acatcggcga ccttgacctt gccgccgtgc
     3001 tggatggcat gacccgcatg tggtttgccc tcgccctcgc ccagggccgt ggacgcagaa
     3061 tcgctttcaa attaatggag gagtaatggc atctgtcggt tattctggaa ctccgcttgc
     3121 aaagaaactc ggcctcaagc ccacactgac ccttttgacg atcaatgcgc cgaaggaata
     3181 tcgttcatgg ttgggagaat tatcgggcgg ggtatcgttg gttgaaacgg ccagagagcc
     3241 gtatgaggca gttcatgtct ttgccacgga gagcgcgttt ctggatggga tattgtccaa
     3301 gctgcgcagg gaattgaagc aggatggttt cgtgtgggtt tcctggccga agaaatcatc
     3361 caaagtgcca acggatatca ccgaagacgt catccgcgag atcgcactgc cgcttgggtt
     3421 tgtggatatc aaagtatgcg ccgtgagtga agtatggtca gggttgaaat tggtgattcg
     3481 taaatcagag cggagttgac atccgtattc ggctagttgg cttgcacctt catgtaggcg
     3541 atcaacccaa ggatggtgag tgcaactccc acaaaaccga tcggcccggg gaatgcgccg
     3601 tgtaaaaaaa cgatctcgcc gatcaaggaa aataccactt ccatggattg ggtggcatcc
     3661 acggcggcga tctcgtaggg ctggtggcag agatggcggg cgtaaagaaa aagggtcgtg
     3721 gcaatgacgc ccgacaacag agcgacaagc gccgtgctga gcaattgtcc gctggcgggg
     3781 gcggggggag aactaaacag gagaatcaac acccaaaacg ggatcgagcc caaggtcaag
     3841 agaaggactc gggcgaagcc attttccaga atcggatttt cgattttggg aatccttgcg
     3901 ttgtgaccca tgcgcgcctc ccaaaccagt tgattgccaa tggggtaggc aaaggcggcg
     3961 atcaaaacag gcagggcgct gaacagcact tcacgcaaac tggtcaacgc ggcatgttca
     4021 atgttgacga ggatgatccc cacaaaaatg attcccgtga aaagcaaacc tttggtgggc
     4081 accttgcgcc cgaaaaagac caacacgatg ggggtggcaa ggatggtggt ctgccatgtg
     4141 gtggcaacga cccagcccgc ggcatattgt gaactgaacg tgatgagtga gtaaaaaaca
     4201 ccgaagccaa tgctgcctgc aagaatccag aacagccaat gactcagaaa gacgcgcctt
     4261 acttcagcca gcgctttttt gccctgtgtg gcgagcagca ataagatcaa gaagatcagc
     4321 atgaacccga agcgcagact ggatgtccac gcccagtgac cgccctgtaa gctcatggcg
     4381 cggttgagga cgaaggtgct gctgaagaat aaagcggcaa ggctgccgat tgcgatgagt
     4441 cgtgtcattt aatttctact gcctcctgtg ccatatttga taaagacgaa gatcaacgga
     4501 attcccaccg caagatacac accgaaatac gccagtccgc ctgggcgctt ggcaaaatcg
     4561 aagaggcgga tgtatataaa tgcagcggta gtgataaaga caaccagcgg atacgccgcg
     4621 aaggaatggt gcaggatggc gttcaggttt ctgtcccaaa agtagggcac aactcccagt
     4681 gcaagcgcaa aatagaaaac cccgaaactc cgcaaggtga acaatgacat gacttcaggg
     4741 aagatgcccg cattggtgcc gatatcgccg atctgtgcgg ttaatccata caagccgagg
     4801 aagccaacga acagtacaaa tgcaaatgcg ggcaggctct gaattttcgt gaaagttggt
     4861 ccgttggatt ggttggacgg acgaatacga agataatcaa aaacaccgac gaatgccgca
     4921 agagcattga cgatcagcgt gatgaaatac aaccaggcaa tcggatgttc aagtttcaac
     4981 ttgtcgcgga agacgaacag cacgatcaac tcaccgatgc cgaatagcca tagataaacc
     5041 agcgaggagt aaaccagttt ccactcccag cggcgggcgt tgatataagc cagccatgca
     5101 ttgccgagac accagccgcc gatggtgatg gtcatgaagg cggttacttt ccacgcgaag
     5161 acaggcgcaa gttgtgatgg gaataaaaat aaaagcacgc ccaatattgc atacagtaaa
     5221 gcacaggtgt aagtcagcca gcgggaaagg ttcgatagca tggtttctcc aattttctgt
     5281 agataaagct gtgccctatt gtagcagtta cttctcttta tctttcgtaa aataaaaagt
     5341 caccagctga ttggactgct gatgactttt tatttaatct ttatcattgc gaggagggtg
     5401 ctttcccaac gaacaaggtc tctccttgcg ctgacgtatg ctaaagcggt tcaaaccgtg
     5461 cggtgaaatg cctgagcagt tcaggtgcga aggtgaattt gtagccgcgc actttgtcgg
     5521 cgcgggattt gatttgagcg gcgcaatctg ccacatagtc catgtggctt tgtgtgtacg
     5581 tgcggcgcgg aagggcaagc cgcagcaatt ccagcttggg gtggatcatt ttgccagaat
     5641 caggatcggg gtaggcgaac atcaccgagc cgatctccac gccgcggatc gcgccttcgc
     5701 ggtacaactc cacagccagc gcctgcccgg ggaattcgct ctgcggaata ttcgggaaga
     5761 cgtgcgccgc atcgaggtaa acagcatgcc cgcctgcggg ttgaatggtg gggatgccag
     5821 cctcgttgag ccgcgccgca agatacgcgg tttgcgtaag ccgtgaggcg agataggctt
     5881 catccagccc ttcgtacaaa ccgaccgcca tcgcgtcgag gtcacggcct gccagcccgc
     5941 cgtaagtggg gaaaccttcc cgcaggatca attcgtttct gatcgcgccg aacaaggttt
     6001 catcgtttag gcacaacagc ccgccgatgt tgacgatggc atcctttttc gcggacatgg
     6061 tcatgccgtc ggccagcgcg tacatttcac gcgcaatgtc gatcaccgat ttgttttcgt
     6121 atcctttttc gcgcagtttg ataaagtagg cgttctcggc atagcgcgcg gaatcaatga
     6181 agaaggggat gttgaactcg cgatagacag ccgccaccgc tttgagattt tccatcgaaa
     6241 caggctgacc gcctcctgcg ttgtttgtaa cggtcaccat tccgaagggg atattcgcca
     6301 ccccgacctt ttgaatgaag gcgcgcagtt tgttgatatc catgttgccc ttgaagggat
     6361 gcggattcgc agggtcgaag gcttcgtcaa tgacgaggtt ggtggggcgc ccgccgcgcg
     6421 cgcggatgtt ggcatcggtg gtgtcgaaat gcatgttcga agggatgtaa ttgcctggtt
     6481 tcaccagaca ggccgagagg atgttttccg cggcacgccc ctgatgagtg gggacaaaaa
     6541 acttgaaccc gaaaatatct tccacggcgg ttttcaagcg ttggtacgag cgcgcacccg
     6601 cataggactc atctccgcgc atgatcgctg cccattgttc ctgactcatg gcgcctgtgc
     6661 cagagtcggt cagcaggtca atgaagatgt cctctgcttt cattgagaat aggttataac
     6721 ctgcctcttt cagcgcgatc tcccgcgctt caggcgagat caggcggatg ggttccgtga
     6781 ccttgatgcg gtacggttcg ggggggtaaa acttgggggt catttgggtc tcctgaaatt
     6841 tgaggttttg tcaaattttg ggtagcgagc ctggcatgtg ttcccctgat ggccattcgg
     6901 tctccaatga cgggcgtatt ttacaagtaa tctacatttt gaggttgtaa caaatgtcat
     6961 gaattcccca tatttgtttg atgtcattgc gagggcggtg catttcgccc gaagcaatcc
     7021 accattaatg aggagattgc ttcgctcgcc caaagggctc gctcgcaatg acatatttta
     7081 tctttctgac ttttgcaatg ctctttcgat ctccgccagc atttcctgcg cggactgcaa
     7141 tactggatga cgcaggcgcg tgtaaatctc aacggcgcga cgggataata ataatgcctc
     7201 gtgtagggac gacccttcac ggtcagacaa gtcgttttct tgcgcttcta cattgccaga
     7261 caaggcactt ttttgcacag ggcgggtcgc ccctacagca tcgacggggt tttgtttgaa
     7321 cagggctttg gcgaggaggt gacaatcgct cgcaatcaac tcctgctgcc cgactttttc
     7381 tgccagcgcg agggcttcgc gggcgaggga ctcggcttct gcccactgct cgcgggcgaa
     7441 ggcgagtcct gctagattgc cagtaattgc tgctatgcca tcctgtctat cgctaatctt
     7501 agcaatttga agggcttcgc gataatcacc ttcagctgag ttgtaatctt tatttgcttc
     7561 ttttgcagtg gcgatgtgag ttaacgtaac agacaccgat acgcttgtgg gagaaatgct
     7621 acgaaagagt tcaagcgatt cactataggc ttctatagcc cttgcataat ccttgttaag
     7681 ttgatggccg accccccctc aatttgattg catttgcttt tttccctgga gtgctgtctg
     7741 accaataatc ggcggcgcgg ttggcacacg ccaaaacctt gtttgattga tttctgagag
     7801 agtaggccca tcctgctaaa tacgccctca aacctgctgt ctcacggtcg ccagcagcaa
     7861 tggcgcgttc ttcggattcc tcattcagcc aaagcaaatc gtcccatttc cctgtgaagt
     7921 caagaaactt atcgaggcta ttgcatacct tttgtaggcg gttgttatcg cctgtgagca
     7981 ggcgcggcag ggcggcggag atgaaatccc attcggtgtc cagcatgggg aatttttcgt
     8041 agtctttgct tccaccgccg tattgca
//
LOCUS       DAOQLC010000157         7959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3661_length_7959_cov_18.463310, whole genome shotgun sequence.
ACCESSION   DAOQLC010000157 DAOQLC010000000
VERSION     DAOQLC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3661_length_7959_cov_18.463310"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PL000_18765"
     CDS             <1..252
                     /locus_tag="PL000_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90981.1"
                     /translation="IGLAPYADSTERARIGADLLQVPLIESVLAPCWSCGGMVAKHPD
                     QVDADSCWPPQHASENACCCVKEVKRSNSGIALSAHSAR"
     gene            257..634
                     /locus_tag="PL000_18770"
     CDS             257..634
                     /locus_tag="PL000_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90982.1"
                     /translation="MPYTVIYNSELHVVEAKLQGDMTVGEVEEIITRIAQTAKEKGCR
                     SIFTDFREVKQKLSILEIYKLPDRTRNIFASFGFDVSLFRRANIAADDLDDYIFHENV
                     MVNRGQNEKVFTDIHNARKWLIG"
     gene            732..1691
                     /locus_tag="PL000_18775"
     CDS             732..1691
                     /locus_tag="PL000_18775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC90983.1"
                     /translation="MNTGLIWLGRVVGLIVALALVGGIYEAVAEAADAKAYPPPGQLV
                     DVGGYRLHINCTGTGGPTVVIDAGLGDWSTGWAWVQTDVSQETRVCTYDRAGYGWSDE
                     GPLPRNAEQFAKELHTLLHNANIPGPYVMVGHSLGGLTARVFTGMYPAEVAGVVLIDS
                     MSPRQFKQSSGNPVAESNLRSRPFVPFTLLAHIGLVRLLAGPLGLIPNLPSDAQKMYL
                     AKMSRATYLQTYFGDESRGMLESRGQAEAVTSFGDLPLIVLTAKLNDMSGWQDWQTEL
                     LQLSSNSQQLFAESGHTIEIDKPDAAVTAILKMVERVRLSSQK"
     gene            2114..>3401
                     /locus_tag="PL000_18780"
     CDS             2114..>3401
                     /locus_tag="PL000_18780"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC90984.1"
                     /translation="MINLSGRQRMLIQLMTRLAIEHQDDETKATLAQAETTFEQTLSA
                     LENGGETIYLPDDFVTLPQTNDVEIKAALSRVREDWGRFRISMDAIQSMPQGDIELQS
                     AVQDLEAQSALLAQQADRVVQLYEASATTKLNILRNIQIIFLLLALVLLGVGAAVTRQ
                     SLLRPLTELGAAAARLGENDLEYPVQVQGPEEMRNLSQSFDGMRVNLNSARQELIDLN
                     NNLEKRVFQRTQELEALNIVSQEITSRLDIEQVLNSVAEKSRALMDGEVSFLCLLDAN
                     QQHLNLQTFSGAADAAIGDTTVISAHDLTTTIVSSEKAVVCSIDTCRDGCSILSQKYR
                     GSHLAASLHSSGQIIGALCVGSSSPQRFGEESAEMLTKLANTAAIALENARLYEQAER
                     IATLEERRRIAAEMHDGLGQTLSYLGLMTDQVVDFLS"
     assembly_gap    3402..3501
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3502..3758
                     /locus_tag="PL000_18785"
     CDS             <3502..3758
                     /locus_tag="PL000_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90985.1"
                     /translation="TNAVNHAHAAHINMSFSQVDEGYVLKIEDDGGGFDSSQPRPAGH
                     FGIQIMKARALRVGGVLTVNSAPRQGTQVSLCFPAEKTGN"
     gene            3762..4421
                     /locus_tag="PL000_18790"
     CDS             3762..4421
                     /locus_tag="PL000_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC90986.1"
                     /translation="MEKIRVLLVDDHALFREGLAAVISSQPNMEVVGNANDGLEAVIK
                     AQELKPDLILMDIQMSTMDGLESTRQIKQILPDTTIVVLTVRDDDEKLFEALKSGAQG
                     YLLKDIRSHKMVEMLQSAMNGEAAITPSLAGRVLAEFRRMSLNQPGSSVADHEDNSLT
                     ERELEVLSLVTKGASDKEVAEKLFVSLNTVKSHIRNILSKLRVNSRKEAAKLAQSKGL
                     L"
     gene            4579..4731
                     /locus_tag="PL000_18795"
     CDS             4579..4731
                     /locus_tag="PL000_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90987.1"
                     /translation="MPENKKEEPQHVGQKIFDNIWLLFVLSLLISTVLYNVWGIIDLM
                     NVPFAK"
     gene            4752..5276
                     /locus_tag="PL000_18800"
     CDS             4752..5276
                     /locus_tag="PL000_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC90988.1"
                     /translation="MLAPEKNWWKPMGRMEKSWLTVALVWCIFLTIMMPLWYFYGKQN
                     VPTETYRTSPADYGAKVNAFVEQYTVGKDEATGFPIVAAPAGSDIYLRASTWQWYPVL
                     QLEKGQEYRLHISSMDLQHGFSLQPVNINLQVVPGYDYVATITPTSTGDFTIVCNEYC
                     FVGHHTMVGKIIVK"
     gene            5288..>6432
                     /locus_tag="PL000_18805"
     CDS             5288..>6432
                     /locus_tag="PL000_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HNC90989.1"
                     /translation="MTSLAPTTPWSAGAKDDYRDCPVTTLKVDMHTERLIIANAVMAV
                     VTLTLGGIAALLIALTRWQAIHLLPAAWFYRLLTLHGIDMLVAWIVFFEMAGLHFGST
                     VLLNARHASPKLAWAGFGMMTVGGLMANAVVLIDPNSSVMFSAYVPLKAHPLFYLSYI
                     LFAVGALITVITFFINIVVAKREGRFTGSLPLVVFGLMTAAILATYTLLEGAAAIVPA
                     FFWSLGILKTYDPGIYRNFFWGFGHPAQQVNLAAMVSIWYALAQMTVGIRPVNEKFSR
                     LAFILYLFFINLGSAHHLLVDPGLSFAWKAVNTSYAMYLAVLGSMMHAFSIPAALEIA
                     LRAKGHRKGLFGWLRNAPWAEPGFSSLVLSMFLFGWIGGVTGVTIGT"
     assembly_gap    6433..6532
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6650..7315
                     /locus_tag="PL000_18810"
     CDS             6650..7315
                     /locus_tag="PL000_18810"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC90990.1"
                     /translation="MTASFLLGLLGSLGHCVGMCSAVILLFDRQPIFSDKRAWALAHA
                     GRITTYTILGFVFGAFGQTLWTFGNLQAALSISFAIIAFYMASAFIGLTPSPELLFSG
                     LIKRWGNAIRTFKSASLPTSYLLGLLWGLLPCGLVLTALLTAVTSANALHGALNMTVF
                     GIATIPSLFAVKWLATKSSSRFLSRGLASLVMMFFGFQFAMRGFASLGMVDHLMLGSF
                     MLW"
     gene            7319..>7959
                     /locus_tag="PL000_18815"
     CDS             7319..>7959
                     /locus_tag="PL000_18815"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNC90991.1"
                     /translation="MTLASASSIPSKALVACSLCGLTTLHPLTNDNGDVFCCPSCREV
                     AALLAESPAIEQKQVTVEGRSDEVILTLGGMWCSSCAWLVSENLKRTKGVVSAEVSFI
                     QQQANITFDSSITNYQSLKRRVRSLGYQATLPNEKPRDEEESFFTRLLIGGVMVLHDM
                     IVGGGIYARELLGWASPETAWLVHFFQIMMLVTSIPVLVLLGLPILRAGFASLL"
BASE COUNT         1786 a         1909 c         2069 g         1995 t
ORIGIN      
        1 ataggccttg ccccctatgc agattcaacc gaacgcgccc gcatcggtgc ggacctgtta
       61 caggttcccc tcattgagtc ggtccttgct ccatgctgga gttgcggagg aatggtggca
      121 aagcatcctg accaggttga tgctgattca tgctggccgc ctcaacatgc cagtgagaac
      181 gcatgttgct gtgtgaaaga ggtcaaaaga tcgaacagcg gtattgccct ctctgcgcat
      241 tcggcgaggt gaaatcatgc cttatacggt tatctacaat tcagaattac atgtggttga
      301 agcaaaactg caaggggata tgactgtggg cgaagtggag gaaattatca ccaggatcgc
      361 gcaaacagcc aaagagaagg ggtgccgatc cattttcacc gattttcgtg aagtgaagca
      421 aaagttatcg atcctggaaa tttataaatt gcctgatcgc accagaaaca tcttcgcctc
      481 attcggcttt gatgtatcgc tctttcgacg ggcaaatatc gcagcagatg acctggatga
      541 ttatattttc cacgaaaatg tcatggtcaa tcgcggacaa aacgaaaagg tatttactga
      601 tattcacaac gcaaggaaat ggcttattgg atgatcgtgc cgcctggcaa ggccagcgat
      661 gttccctccg gggtggatct aacccctgga gaagatattg cgaaagcaat atcaaccaaa
      721 aggaaaaaca aatgaacaca ggtttgatct ggctggggcg ggtcgtgggg ttgatcgtag
      781 cattggcatt ggtggggggc atttacgagg ctgtggcaga ggctgctgat gccaaggcat
      841 atccgcctcc cggtcagttg gtggatgtcg gcggttatcg cttgcatatc aactgcaccg
      901 gtacaggtgg cccgactgtg gtcattgacg ctggtctggg tgattggtct acggggtggg
      961 cctgggtaca gacagatgtc tcgcaggaga cacgagtttg cacctacgat cgggctggat
     1021 atggttggag tgatgaggga cctctgccac gcaacgcgga gcagttcgca aaagaattgc
     1081 ataccttatt gcataatgcc aatatccctg gcccatatgt gatggtggga cactcgcttg
     1141 gcggcctcac tgcacgggtg tttaccggca tgtatccagc agaggtcgcc ggggtcgtgc
     1201 tgattgactc gatgagtccg aggcagttca agcaatcttc tggaaacccc gtagcggaga
     1261 gtaacctgcg gtcgcgtcca tttgtgcctt tcacgctgtt ggctcacatt gggcttgtgc
     1321 gcttgcttgc aggaccactc gggctaattc caaacctgcc atcggatgcg caaaaaatgt
     1381 acctggcgaa aatgtctcgt gcaacatatt tacagaccta ttttggtgat gagtctcgtg
     1441 gaatgttgga aagcaggggg caagcggaag ccgttacatc ttttggcgat ctgcctctga
     1501 tcgttctgac cgccaaactt aatgacatgt caggttggca ggattggcag accgaattgc
     1561 tgcaattgtc gtccaacagc cagcaacttt ttgcagagag tggtcatacc atcgaaattg
     1621 acaagcccga tgcggctgtc acagcgattt tgaaaatggt tgaacgggta cgcctgtcaa
     1681 gccagaagta gatgattgcg gcagggtcag tatggtaaga gtttgtcatg atacagggcg
     1741 ggcggattcg gttttggggt gtatgaaaaa cagtttgatc gataatcctt ttgttttacc
     1801 ccatcattaa ccagggagta tccacttcga gataacaagt ccaaaagagt gtcgttccga
     1861 gtctcatttt tgttttttgc ggcgaagatt ctataggata acttgagaga ccctcatctt
     1921 ttcggcttcc agttcggcaa taccctccgg tgtataattc caaaatcaaa atcaacctgt
     1981 ttcaaaaagg tggatggtgc ttaagagtct gcaaggtcgg ttaggtgctt tattcattgc
     2041 atttgttttg cttgtgctta cttcggttgg cgcaacattt tggggtgtcg agacccagca
     2101 aaaggatgcg cttgtgatca acctctcagg gcggcagagg atgttgatcc aattaatgac
     2161 aaggctggcg atcgagcatc aggatgatga gacaaaggca acgctggcgc aggcggaaac
     2221 gacatttgag caaacacttt cagcattgga aaacggcggc gagaccatct atctgccaga
     2281 cgattttgtg accctgcctc agacgaacga tgtagagatc aaggcggcat tgagccgtgt
     2341 gcgggaagat tgggggcggt tccgcatatc catggatgcg atccaatcca tgccgcaggg
     2401 tgatattgaa ctgcaatctg ccgttcagga tctggaggcg caatctgcct tgctggcaca
     2461 acaggcagac agggtggtgc aattatatga agcctctgcc acaacaaaac tgaacatcct
     2521 ccgaaatatt cagatcatct ttttgcttct ggcgttggtg ttgcttgggg tgggggcggc
     2581 ggtcacgcgt caatccctgc ttcggccgtt gacggagttg ggcgctgcgg ctgcccgtct
     2641 tggagaaaat gacctcgaat acccggtgca ggttcaaggt cccgaagaga tgcggaactt
     2701 gtctcaatcc ttcgatggga tgcgcgtcaa cttaaattcc gcccgccagg aattgatcga
     2761 cctcaataat aatttggaga agcgtgtctt tcaacggaca caggaattgg aagcgttgaa
     2821 tatcgtcagc caggagatca cctcgcggct tgatattgaa caggtgttga attcagtggc
     2881 tgaaaaatca cgcgcgctga tggatggtga agtctctttt ttatgtttgc tggatgccaa
     2941 ccagcagcat cttaatcttc aaacgttcag tggtgcagcg gatgcggcta tcggggatac
     3001 gacggttatc agtgcccacg atctgacaac caccattgtg agcagcgaga aggctgtggt
     3061 ttgcagcatt gatacctgcc gtgacgggtg ctcgatcctc tcacaaaaat atcgggggag
     3121 tcatctggca gcttccttgc attccagtgg gcagatcatc ggtgcattgt gtgtgggcag
     3181 ttcaagtccg cagcgtttcg gagaagagtc ggcggaaatg ctgacgaaac ttgcaaacac
     3241 cgccgcgatc gcattggaaa atgcacggct gtatgaacag gcggaacgaa ttgcaacact
     3301 ggaagagcgc cgccgtattg cggcggagat gcacgatggg ctggggcaga ccttgagtta
     3361 cctcgggttg atgaccgatc aggtggtgga ttttctttcc annnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn ntgaccaatg cggtcaatca cgcccatgcg gctcatatca
     3541 acatgagttt cagccaggtt gacgaggggt atgtattgaa aatcgaagat gatgggggcg
     3601 gtttcgactc ttcccaaccc cgccctgcag gacactttgg catacagatc atgaaagcgc
     3661 gtgcattgcg agtcggcgga gttttgacgg tcaactccgc gcccaggcag ggtacacaag
     3721 tcagcttgtg cttccccgca gaaaaaacag ggaattagtt tatggaaaaa atacgcgtgt
     3781 tattggtgga tgaccatgcc ttgtttcgtg aagggctggc agcggtgatc agttcacagc
     3841 ccaatatgga agtggtcgga aatgccaacg atggattgga ggcggtgatc aaagcacagg
     3901 agcttaaacc agacctgatc ttgatggata ttcaaatgtc aaccatggat ggtttggaat
     3961 ccacccgcca gatcaaacag atcctgcctg ataccacgat cgttgtgctc actgtccgcg
     4021 atgatgacga gaaattattt gaagcgttga agagcggcgc gcaggggtac cttctaaagg
     4081 atatccgctc gcataaaatg gtggagatgt tgcaaagcgc catgaacggt gaggctgcta
     4141 ttacaccgtc gctggcgggg cgggtccttg ccgagtttcg caggatgagc ttgaatcagc
     4201 caggcagttc cgtggcagat catgaagaca actctttgac cgagcgggaa ctggaagtgc
     4261 tttcccttgt tacgaaaggt gcttcagaca aagaggtggc tgaaaaatta ttcgtgagtt
     4321 tgaacacggt caagagccat atccgcaata ttctttcaaa gcttcgtgtc aattccagaa
     4381 aggaggctgc gaagctggct caaagcaaag ggttgcttta gttgaaaaag aactaattct
     4441 ttttcaaaaa agagtgatgt aaaaatcact ctttttttat tgcaatgatt acatataaca
     4501 attcgtttat cggggcaatt acagtaatat gggttatgcc attctgcatt tttcaatcac
     4561 atcatgagga gaattgccat gcccgaaaat aaaaaagaag agccgcagca tgtgggtcaa
     4621 aaaatttttg acaacatctg gctgctgttc gtgttgtcgc tgttgatcag caccgtgctt
     4681 tacaacgtgt ggggaatcat tgacttgatg aacgtcccgt ttgccaaata aaagggttgg
     4741 aggaatagac tatgttagcg cctgaaaaaa attggtggaa gcccatgggg cggatggaaa
     4801 aatcctggct cacggttgcc ttggtctggt gcatttttct gacgatcatg atgccgctct
     4861 ggtattttta cggcaagcaa aatgtgccca cagaaaccta ccgaacctcg ccagcagact
     4921 acggggcgaa ggtcaatgcc tttgtggaac aatacaccgt cggcaaggat gaagcgactg
     4981 gtttcccgat tgtcgccgcg cctgcgggtt cagatatcta tctgcgtgcc agcacctggc
     5041 agtggtatcc tgtcttgcag cttgaaaagg gacaggaata ccgcctgcac atctcttcaa
     5101 tggatttaca gcacggcttt tctcttcaac ctgtaaacat caatcttcaa gttgtgcctg
     5161 ggtacgatta cgtagcgacc attaccccca ccagcactgg tgatttcacc attgtttgca
     5221 atgaatattg ttttgtcggt catcacacga tggtgggcaa aatcatcgtg aaataggaga
     5281 aacacagatg acatctcttg ctcccacaac tccctggtcg gcaggtgcga aggacgacta
     5341 ccgcgattgc ccggtaacca ccctcaaagt ggatatgcac accgaacgcc tcatcatcgc
     5401 taatgcggtg atggcagtgg tgacacttac tctcggcggc attgcggcat tattgatcgc
     5461 ccttacccgc tggcaggcga ttcatttact acccgctgct tggttctacc gattattgac
     5521 cttacatggc atcgatatgc ttgtcgcgtg gatcgtgttc ttcgaaatgg ctggtttaca
     5581 tttcggttca accgtgctgc tgaacgcgcg tcacgcttcg cccaaactgg catgggcagg
     5641 tttcggcatg atgaccgttg gcggcttgat ggcgaatgcc gttgtgctga ttgacccgaa
     5701 ttccagcgtc atgttctccg cctatgttcc gctcaaagcg cacccgcttt tctacctcag
     5761 ttacatcctc ttcgcggttg gtgcgttgat caccgtcatt accttcttca tcaatattgt
     5821 cgtcgccaaa cgcgaaggac gcttcacggg ttcattgccg ctggtcgtct tcggtttgat
     5881 gaccgccgcc attctcgcca cctacaccct cctcgaaggc gccgccgcca tcgtgcccgc
     5941 cttcttctgg tcactgggta ttctcaaaac ctacgacccc ggtatttacc gcaacttctt
     6001 ctggggcttc gggcatcccg cccagcaagt caatctcgcg gcgatggttt ccatttggta
     6061 cgcgctcgct caaatgaccg ttggcattcg ccccgtcaat gaaaagttct cacgcctcgc
     6121 tttcattttg tatctgttct tcatcaatct tggttcggcg catcacctgc tagtggaccc
     6181 tggtctttcc ttcgcttgga aagccgtcaa cacgtcctac gccatgtatc tcgccgtgct
     6241 cggctcgatg atgcacgcct tctccatccc tgctgcattg gaaattgccc tgcgcgccaa
     6301 gggacacaga aaaggtttgt tcggctggct tcgcaatgca ccgtgggcag agcccggttt
     6361 tagttcactc gtcctttcca tgttcctctt tggttggatc ggcggcgtga cgggcgtcac
     6421 catcggcacc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggtgta
     6541 ttacttcacc aactggtggc tgcttggtcg tacctggttt attaactaag ccagaattct
     6601 cctttgacat cctgcccggg gatcgccacc tcgggcagga acccttaata tgaccgcatc
     6661 atttctatta ggattactcg gtagccttgg gcattgcgtg gggatgtgca gcgctgttat
     6721 tttgctcttc gaccgtcaac cgatttttag cgataaacgt gcctgggctc tcgcacatgc
     6781 agggcgcatc acaacctata cgatccttgg attcgttttt ggcgcgttcg gtcaaacctt
     6841 gtggacattc ggaaacctgc aagccgcgct ttcgatttca tttgcaatca tcgcctttta
     6901 tatggcatcc gcatttattg gattaactcc ctcacccgaa cttttatttt ctggattaat
     6961 taaacgctgg ggtaacgcca tccgcacttt caagtccgct tcgctcccaa cctcgtacct
     7021 gctcggtctt ctgtggggac tcctcccctg cggcttggtt ctgaccgcgc tcctcacagc
     7081 ggtgacatcc gccaacgcgc tacacggcgc actgaatatg accgtcttcg gcattgccac
     7141 aatcccaagt ttatttgccg tcaaatggct ggcgacaaaa tcctcctccc gcttcttatc
     7201 tcgcggactt gcctcgcttg tgatgatgtt cttcggcttc caattcgcca tgcgcggatt
     7261 tgcctccctc ggcatggttg accatcttat gcttggctca ttcatgcttt ggtaaacaat
     7321 gactcttgca tctgcttctt ctatcccttc aaaagcactc gtcgcctgtt ccctgtgcgg
     7381 actgacgact cttcatccgt taacgaatga caatggggat gtattttgct gtccgtcgtg
     7441 cagagaggta gcggcgcttc tagcggagag tccagccatt gaacagaagc aggtcacagt
     7501 cgaaggaaga agcgacgaag tcatcctcac gctgggtggc atgtggtgtt cgtcttgcgc
     7561 gtggttggtg agcgagaatc tcaaacgcac aaaaggcgtg gttagcgcag aggtgagttt
     7621 catccaacaa caagccaaca tcaccttcga ctcatcaatt accaattacc aatcactaaa
     7681 acggcgcgtg cgctcgctgg gttatcaagc cacactgccc aacgaaaagc cgcgcgatga
     7741 agaggaatct tttttcacgc ggttgttaat cggcggcgtg atggtgctgc acgacatgat
     7801 cgtgggcggc ggaatttatg cgcgtgaact gctcggctgg gcaagcccag agacggcgtg
     7861 gctcgtgcat tttttccaga tcatgatgct ggtcacgagt attcctgttt tggttttgct
     7921 gggtctgccg attttgcgcg cagggtttgc aagcctgct
//
LOCUS       DAOQLC010000158         7638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3894_length_7638_cov_25.417777, whole genome shotgun sequence.
ACCESSION   DAOQLC010000158 DAOQLC010000000
VERSION     DAOQLC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_7638_cov_25.417777"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="cysS"
                     /locus_tag="PL000_18820"
     CDS             <1..1221
                     /gene="cysS"
                     /locus_tag="PL000_18820"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC90992.1"
                     /translation="AVKHVMNYTDVDDKIINRANQLGEDPLKLSQRYIEDYANDLKSL
                     NVLPATSNPQVSKTMPLIIQFIEGLIQKGHAYAATNGDVYFRVTSDDDYGRLSGRKLE
                     DMQAGARIEIGEAKDHPMDFALWKAAKPGEISWDSPWGKGRPGWHIECSAMNLAELGE
                     QIDIHGGGNDLIFPHHENEIAQSESYTGKEFSRYWIHNGMLQLGGEKMSKSLGNIVSI
                     KEFLSRRSADVMRMLVLNGTYRAPLMFNEDTLDAAEKNVERLKSALRPASASSQGLSA
                     EAASALTSASESAKANFTEAMDNDFNTAGAVAALFELSKAINTARDNGATGDQLKAAQ
                     TMFGELAGVLGLKLEEKKGSSEVEAQVIALIAERTEARKQKQWKRSDELRDQLKEMGV
                     TIEDSKDGTTWRWG"
     gene            1291..2736
                     /locus_tag="PL000_18825"
     CDS             1291..2736
                     /locus_tag="PL000_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC90993.1"
                     /translation="MPTLMLPKSGKPKADILESMRRARDKDAQWQNGKVFGLVYHISE
                     EIDDLLKEAYTMFFSENGLNPTAFPSLSKFENEIIAMASNLLGGDDKVAGTMTSGGTE
                     SLLMAVKTARDYARAKRGITKPEIIMPSTAHPALDKAGEYFNVRMIHIPVREDLRADV
                     DATRRAITPNTIMLVGSAPSYPHGVVDPIRELAALAQEHGLLFHTDACVGGFMLPFVR
                     KLGYPVPEFGFDVPGVTSISADLHKYAYAAKPASLVLYRTSELRRHQMFVSTDWPGGI
                     YPSPSMAGSRPGGPVAAAWALLNYLGEDGYLEITDIVMRTAKILQDGVNEIPGLKVLS
                     NPEMSVFAIASDSMDVYELADELALRGWHLDRQIFPPSLHVTVNYVHAQVGAEFLTDL
                     ANAAERVRRPGLRKTATKFLVQAANLLTRVLPEETVSNLMDKASSLLGGGGGLPARMA
                     PMYGLIGSLPNRGDLKELVLDLLDNINSYKE"
     gene            complement(2737..3204)
                     /locus_tag="PL000_18830"
     CDS             complement(2737..3204)
                     /locus_tag="PL000_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC90994.1"
                     /translation="MKKEFTIATISTKDSPFINDLCKLRDGLSAHHDDTTSSERFRQF
                     MLKHLGEETMLTFLILNNGRPIGYGLAFDVIEHAYMPEWTRKGYITQFFVNAEYRGQG
                     IGEMGLAYIHDWFRARGITDVLLNVAIKNEVGNRFWQKNGYTPYATRMICRLK"
     gene            3259..>3941
                     /gene="rlmB"
                     /locus_tag="PL000_18835"
     CDS             3259..>3941
                     /gene="rlmB"
                     /locus_tag="PL000_18835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNC90995.1"
                     /translation="MKELIYSRNAVYEVLRAKRRQVFSIEIAEGVQEKGRLTEIVKMA
                     QQQKIRISRVPRPKLDKVHQNNQGVVAEVSGYPYSDVVEILEQAKEELPFVLILDSLQ
                     DPQNFGTLIRTAEAIGVHGVVIPLARSVDVTPAVVNASSGASEHMLIAQANLSQTIDA
                     LKDNDVWIVGLDQAGAEVEAGSRHLKGALGLVVGSEGEGLHDLVRKKCDIVLKLPMKG
                     RIESLNAAVA"
     assembly_gap    3942..4041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4042..5328
                     /locus_tag="PL000_18840"
     CDS             <4042..5328
                     /locus_tag="PL000_18840"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNC90996.1"
                     /translation="WGTATASHQVEGNNTNNNWHAWEEAGHTVHKSGLAADWWGGRWR
                     EDFDRAAETGQNAHRLSLEWSRIQPTPDTWDEEAIEKYRTMLRGLRERNMTALVSLHH
                     FTDPLWVTERGAWENAEIVPLFEKYVRKVVGALKEYVTLWCTINEPNVYALGGYVQGA
                     FPPGKKDIRLSMRVLGNMLRGHAAAYRAIHELQPESRVGYALHYRPMVPRLSWSPLDR
                     LMRNIRYNGINMGFPSAISTGVMRSPVGNQNIPEAKGTQDYLGLNYYSVDTVWFDLFK
                     PGELFSNSGYPKEADMSDTNFIANIPEGIFNSIKWIERTYPNLPIIITENGVEDSDDH
                     MRPRYLAQHLHQVWRAVNFNWQVKGYFHWSLVDNFEWERGWTQRFGLWGLDLDTQKRI
                     RRPSVDLYAEICKENGISSEMVQKYCPEVFKKIFPS"
     gene            5430..6011
                     /locus_tag="PL000_18845"
     CDS             5430..6011
                     /locus_tag="PL000_18845"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC90997.1"
                     /translation="MSTREKILDTALTLFNDQGTAAVSTNHIAEAAGISPGNLYYHFR
                     NKDEIIHELFERLFAANDAAFSLSADTFPTLADLQNMVKANYKILWQFRFAHRELAAL
                     LRADGELRSRFLSVRKRGFDGFTQLFAAFTSAGVLRAANPTVIENLAETCWLITEFWL
                     NSLEIGGKAVTEAQMERGVQLMMQSLEPYIAQP"
     gene            6051..>6373
                     /locus_tag="PL000_18850"
     CDS             6051..>6373
                     /locus_tag="PL000_18850"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HNC90998.1"
                     /translation="MSEKTFRKLAGIFFIVGAILVNIPYTLLIINFDYPDILRLPAAE
                     ILTRFQAGGSALIYTWLAFAWVGLPMLLGAIMLKRILADENAPFLETATTLGVIGFIV
                     QVVGLL"
     assembly_gap    6374..6473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6530..>7638
                     /locus_tag="PL000_18855"
     CDS             6530..>7638
                     /locus_tag="PL000_18855"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNC90999.1"
                     /translation="MTHAEQIASQLNVKPSQVTAVINLLDEGNTVPFIARYRKEMTGS
                     LDDEQIRIIADELLRLRTLDERRVSILASIEEQGKLTDELRESINAAVTMTALEDLYA
                     PYKKKRRTRAMVAREKGLEPLAELILKQSTSGSPEKLAVKFLNEQVANIEEALQGARD
                     IVAEMISENANVRAATREKALKFSTVHSVKVADAADEKRVYESYYDFSMRVDRLQPHQ
                     ILALTRGEKEGILRVRVDVPERDWLNAVQSEFEQDIISPFAEQLELAIRDSAERLLLP
                     AIERDVRREKGEAADNHAIQVFATNLRALLGQAPLANQVVLGIDPGFRTGCKIAVVDA
                     TGKLLDTGTIYPHEPKNDWKGAINTLQDMINRHHVT"
BASE COUNT         1805 a         2098 c         1912 g         1623 t
ORIGIN      
        1 gccgtcaaac acgtcatgaa ctacaccgac gtggacgata agatcatcaa ccgcgccaac
       61 cagctcggcg aagacccgct taaactgtca caacgctata ttgaagacta cgccaacgat
      121 ctcaaatcgt tgaatgtatt gcccgctaca tccaacccgc aggtcagcaa gaccatgccg
      181 ctcatcatcc aattcatcga agggttgatc caaaaaggac atgcctatgc cgccaccaac
      241 ggtgacgtgt atttccgtgt caccagcgac gacgactacg gccgcctctc aggccgcaaa
      301 ctcgaagaca tgcaggcggg cgcgcgcatc gaaatcggcg aagccaaaga ccaccccatg
      361 gattttgctt tatggaaggc cgccaaaccc ggtgaaatct catgggacag cccgtggggc
      421 aaaggccgcc ccggctggca catcgaatgc tctgccatga acctcgccga actgggcgag
      481 cagatcgaca tccacggcgg cggcaacgac ctcatcttcc cgcatcacga aaatgaaatc
      541 gcccaaagcg agagctacac cggcaaggaa ttctcccgct actggatcca taacggcatg
      601 ttgcagctcg gcggcgaaaa aatgtccaaa tcgcttggca acatcgtcag catcaaagaa
      661 ttcctttcca ggcgttccgc cgatgtcatg cgcatgttgg tcttgaacgg gacctatcgc
      721 gcaccgttga tgtttaatga ggataccctc gacgccgccg aaaagaacgt cgagcgcctc
      781 aaatccgccc tgcggcctgc ttctgcctcc tcacagggac tcagcgccga agccgcttcg
      841 gctctcacct ctgccagcga atctgcaaaa gcgaacttca ccgaagccat ggacaatgac
      901 ttcaacaccg caggtgctgt cgccgcgctg tttgaactaa gcaaagccat caacaccgcc
      961 cgcgataacg gcgccacagg cgatcaactc aaggccgccc aaaccatgtt cggtgaactt
     1021 gcaggagtcc tcggcctcaa gctcgaagaa aagaaaggct ccagcgaagt tgaggcacag
     1081 gtcatcgccc tgatcgcaga gcgaactgaa gcgcgcaaac aaaaacagtg gaaacgttcc
     1141 gacgaactcc gcgaccagct caaagagatg ggtgtcacca tcgaagacag caaagatggc
     1201 acgacctggc gctggggtta aaaaataaat gttgtgatat tgcagggaca cagcgccgct
     1261 gtgtccctat ttttttaaaa ggagtccttc atgcctacac tcatgcttcc caaatcagga
     1321 aaacccaagg cagatattct cgaatccatg cgccgcgcac gggataaaga tgcccaatgg
     1381 caaaacggga aggtctttgg cctggtctat cacatctcag aagagattga cgacctgctc
     1441 aaagaagcct acaccatgtt cttttcagag aacggactca accccacggc ctttcccagc
     1501 ctgagcaaat ttgagaatga gatcatcgca atggcatcca acctgttggg cggcgatgac
     1561 aaagtggcgg gaaccatgac ttcaggcggc accgagtctt tgttgatggc ggtcaaaacc
     1621 gcgcgtgatt atgcacgtgc aaagcgaggc atcaccaaac cagagatcat tatgcccagc
     1681 accgcacacc ctgcactgga taaggcaggt gaatatttca acgtgcgcat gattcacatt
     1741 cccgtgcgtg aagatctccg cgctgatgtg gatgccacac gccgcgccat cacaccgaat
     1801 accatcatgt tggtcggttc ggctccctca tatccgcatg gcgtggtgga tccgatccgt
     1861 gaactggctg cactggcgca ggaacacggt ctgctttttc acacagatgc ctgtgtgggc
     1921 ggcttcatgc tgcccttcgt ccgcaagttg ggataccctg tgcctgagtt tggttttgac
     1981 gttcccggtg taacctccat ctcagcggat ttgcacaagt atgcctacgc tgccaaaccc
     2041 gcttccctcg ttctttatcg gacctcggaa ctgcgccgcc atcaaatgtt tgtttccacc
     2101 gattggccgg gcggcattta tccttctcca tccatggcgg gttcgcgccc cggcggccct
     2161 gttgctgcgg catgggcgtt gctcaattat ctcggggaag acggttatct tgagatcact
     2221 gatatcgtca tgcgcacggc aaaaatcttg caggatggcg tcaatgagat ccccggtttg
     2281 aaggtgttga gcaacccgga gatgagcgtc tttgccatcg catctgattc aatggacgtg
     2341 tacgaactcg ccgatgaact cgccctgcgc ggctggcatt tggacaggca gatctttcca
     2401 ccgtctctgc atgtgacggt gaattatgtc catgcacagg tgggggcgga gtttctcaca
     2461 gacctggcga atgccgctga aagagtccgc aggcccgggc taagaaagac cgcgacgaag
     2521 tttctggtcc aggctgccaa cctgttgacc agagtcctgc ctgaggagac ggtcagcaat
     2581 ctgatggaca aggcttcgtc gcttcttggt ggaggcggcg gtttgcctgc gcgtatggct
     2641 cccatgtatg ggctgatcgg ttcactgcca aatcgcggcg atttaaaaga attggtgttg
     2701 gatttgctgg ataatatcaa ctcgtataaa gagtgatcat ttgaggcggc agatcatgcg
     2761 tgtggcgtag ggggtgtagc cgtttttctg ccagaagcga tttccgactt cgtttttgat
     2821 cgctacgttg agcaacacat cggtgatgcc gcgtgcgcga aaccaatcgt ggatgtaggc
     2881 cagccccatt tcaccgatgc cctgaccgcg atactcagcg ttgacgaaga actgcgtaat
     2941 gtatcccttg cgggtccatt cgggcatgta ggcgtgttca atgacatcga aagcaaggcc
     3001 gtagccaatg ggtcggccgt tgttgaggat caagaacgtt agcatggttt cttcgccgag
     3061 gtgcttgagc atgaactgcc tgaaacgctc ggatgacgtt gtgtcgtcat ggtgtgcgga
     3121 taatccatca cgaagcttgc acaggtcatt tataaatggg gaatcttttg tgctgatcgt
     3181 ggcgatggtg aactcttttt tcatgctgaa aattatacat tcacagaaga gttgtttctc
     3241 atgtaaaatc aatccaccat gaaagaactt atctattccc gcaatgccgt gtatgaggtg
     3301 ctgcgcgcaa aacgcagaca ggtgttttcc attgaaattg ccgaaggggt gcaggaaaaa
     3361 gggcgtttga cagagatcgt taagatggca cagcagcaaa aaatcaggat cagccgcgtg
     3421 ccacgcccca agctggacaa ggtccatcag aacaatcagg gtgtcgttgc tgaagtaagc
     3481 ggatatccgt attcggatgt ggttgagata ctcgaacagg caaaggaaga actgcctttt
     3541 gttttgatcc tcgattcgtt gcaggacccg cagaacttcg gcacgcttat ccgcacggcg
     3601 gaggccatcg gtgtacatgg tgtggtgatt ccgctggcgc gctcggtgga tgtgacccca
     3661 gccgtggtca atgcatcttc tggcgcgagc gagcatatgt tgatcgcaca ggcgaatctt
     3721 tcgcagacga tcgatgcgct caaagataat gatgtgtgga tcgtcggtct ggatcaggct
     3781 ggggcggagg tggaggcggg atctcgtcat ctcaaaggcg cactcggtct tgtcgtcggt
     3841 tcggaaggcg aagggttgca tgacctcgtc cgaaagaaat gtgacatcgt tttgaagctg
     3901 cccatgaaag gcaggatcga atcgctcaat gctgctgttg cnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn ntggggaacg gccaccgcct cacatcaggt ggaaggcaac
     4081 aacaccaata acaactggca cgcatgggag gaagcggggc acaccgtcca caaatctggt
     4141 cttgccgccg attggtgggg tggacgctgg cgcgaagact ttgaccgtgc cgccgagaca
     4201 ggccagaacg cgcatcgcct gtctttggaa tggagccgca ttcaacccac gcccgatacc
     4261 tgggatgaag aagccatcga aaaataccgc accatgctgc gcggattgcg tgaacgcaac
     4321 atgaccgctt tggtgagcct gcatcatttt acagatccgc tttgggtgac agaacgcgga
     4381 gcctgggaaa atgcagagat cgtcccactg tttgagaaat acgtccgcaa ggtggtcggc
     4441 gctttgaagg aatatgtgac gctttggtgt accatcaatg aaccgaacgt ctatgctttg
     4501 ggcgggtacg tgcaaggcgc attcccacct ggcaagaagg atatccgtct atccatgcgt
     4561 gtgcttggaa acatgctgcg cggacatgct gccgcgtatc gtgccatcca tgaattgcag
     4621 cccgaatcgc gtgtaggcta tgccttgcat taccgtccga tggttccgcg tttgagttgg
     4681 tcgccgctcg acaggctcat gcgcaacatc cgctacaatg gaattaacat ggggttccct
     4741 tctgccattt ccacgggtgt gatgcgttct cctgttggaa atcaaaacat ccctgaagcc
     4801 aaaggcacac aggattatct cggcttgaat tactactcgg tggatacggt ctggttcgat
     4861 ctcttcaaac cgggagagtt attctccaac agcggctacc ccaaagaagc cgatatgagc
     4921 gacacgaact tcattgccaa tattcccgaa ggcattttca actccatcaa atggatcgaa
     4981 cgtacatatc ccaatttgcc catcatcatc accgagaatg gtgtggagga ttccgacgat
     5041 cacatgcggc cgcgctatct ggcacagcat ttgcatcagg tttggcgtgc cgtcaatttc
     5101 aactggcagg tgaagggata tttccactgg tcactggtgg ataattttga atgggagcgc
     5161 ggttggacgc agcgcttcgg cctgtggggg cttgacctcg atacccaaaa acgcatccgc
     5221 cgcccaagcg tggacctgta tgccgagatc tgcaaagaga acggcatctc gtctgagatg
     5281 gtgcagaaat attgccccga agtgttcaag aaaattttcc catcctgata ccaactaaaa
     5341 acagggacgg attccgtccc tgtttttatc ttgacaaatt agagtattta ctctaaaata
     5401 atagagtaaa tactctagga gatgatccca tgtccacacg cgaaaaaatc ctcgataccg
     5461 ccctgaccct tttcaacgat caaggcacgg cggcggtcag caccaatcac attgccgaag
     5521 cggcgggaat cagccccggt aatctgtatt accacttccg caacaaagac gagatcatcc
     5581 acgagttatt cgagcgcttg tttgccgcta atgacgccgc cttcagcctc tccgccgata
     5641 cgttccccac acttgctgac ctgcaaaaca tggtgaaggc aaattacaag atcctctggc
     5701 aattccgctt tgcccaccgc gaactcgccg ccttattacg cgccgatggt gaacttcgca
     5761 gccgctttct ttccgtccgc aagcgcggat tcgacggctt cacccagctt tttgcagcct
     5821 tcacctcggc aggtgtactc cgcgctgcaa acccaacagt gatcgaaaac ctcgcagaga
     5881 cctgctggct gataaccgaa ttctggttga acagcctcga aataggcggc aaagccgtca
     5941 ccgaagcaca gatggaacgc ggcgttcaat taatgatgca atcgctcgaa ccctatattg
     6001 ctcaaccata aacacagtct tattttgttc atcgttcaaa aaggaaacac atgtctgaaa
     6061 aaacttttcg taaacttgcc ggtatatttt tcatcgtcgg cgcgatcctc gttaatattc
     6121 cgtacaccct gctcatcatc aacttcgact atcccgatat cctgcgcctg cccgccgcgg
     6181 agatcctcac cagatttcaa gcaggcggca gcgccctgat ttatacctgg ctcgcctttg
     6241 catgggttgg cctgcccatg ctcctcggcg cgatcatgct caaacgcatc ctcgcagatg
     6301 aaaatgctcc ctttctcgaa actgcgacga ctctcggtgt gatcggattc atcgtgcagg
     6361 tggttggatt gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcatcc
     6481 ttcccccttc acgcgcagcg ttcatccttt ctgataaaat tccaccccta tgacccacgc
     6541 tgaacagatc gcatcccaac tcaatgtcaa gccatcgcaa gttacggctg ttatcaacct
     6601 tttagacgaa ggcaacaccg tcccatttat tgcccgttac cgcaaagaaa tgacaggctc
     6661 tctcgacgat gaacagatcc gcatcatcgc cgatgaactc ctgcgcctgc gcacgctcga
     6721 cgaacgccgc gtctccatcc ttgcttccat cgaagagcag ggcaaactca ccgacgaact
     6781 ccgcgagagc atcaacgccg ccgtcaccat gaccgcgctc gaagatctgt acgcgccgta
     6841 taaaaagaaa cgccgcaccc gcgccatggt cgcccgtgaa aagggactgg aaccactggc
     6901 tgagttgatc ctcaagcaat ccaccagcgg ctcccctgaa aaactcgccg tgaaattcct
     6961 caacgagcaa gttgcgaaca tcgaagaagc cctgcaaggc gcgcgtgaca tcgtcgctga
     7021 aatgatcagt gaaaatgcca acgtccgcgc tgcgactcgt gagaaggcgc tcaagttttc
     7081 caccgttcat tctgtgaagg tcgcggacgc agccgatgaa aaacgcgtgt acgaaagtta
     7141 ttatgatttc tccatgcgcg tggatagact ccaaccgcat caaatcctcg cactcacccg
     7201 cggcgaaaag gaaggcatcc tgcgtgtacg cgtggacgtc cccgaacgtg actggctcaa
     7261 tgctgtgcaa tctgaattcg agcaggacat catcagtccc tttgccgaac aactcgaact
     7321 tgccatccga gactccgccg agcgtctttt gctcccagcc atcgagcgtg atgtccgccg
     7381 cgaaaaaggc gaagccgcag acaaccacgc cattcaagtc ttcgccacca atctgcgcgc
     7441 cctgctcgga caagctccgc ttgcgaatca agttgtcctc ggcatagacc ctggcttccg
     7501 cacaggctgc aaaatcgccg tcgtggacgc cactggcaaa ctgcttgaca caggcacgat
     7561 ctatccgcac gagccgaaga acgattggaa gggcgcgatc aacaccctgc aagatatgat
     7621 caaccgccat cacgtcac
//
LOCUS       DAOQLC010000159         7607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3920_length_7607_cov_24.614539, whole genome shotgun sequence.
ACCESSION   DAOQLC010000159 DAOQLC010000000
VERSION     DAOQLC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_7607_cov_24.614539"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PL000_18860"
     CDS             complement(<1..455)
                     /locus_tag="PL000_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91000.1"
                     /translation="MTGQYRHLGIFSDWVAAAQRQQPLYQNTPPGPRTRELVRETLGF
                     CHTDESPAEVRIEREWQKDGLIGQEVSWSVGFGPRTHAYVFKPANATRTLPGIVALHD
                     HSDFKFYGKEKIADGPEEPSPVLNEFRKTPYGGRAYVNALAREGFVVLV"
     gene            complement(471..1703)
                     /locus_tag="PL000_18865"
     CDS             complement(471..1703)
                     /locus_tag="PL000_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91001.1"
                     /translation="MSNKESKAIICPSCGAPLEAKEEAKRVKCGYCGTLVNISQSAQS
                     ATPVTPPAQTESKTIVDPEDAELDAAEQDIKRSWQIIIAIVAAIFFVVLGVIRSSKPA
                     YRPVPTLRPNNTPAATSTPRPPSLAKHLLTFNLKDSAPENPKDASALVVNSQGNIMVS
                     DTDAGHIRIFDETGKLITTIATNKNAPVTGMAVSTSGEILLVHNLEIHRYNTEGQETG
                     VMKDQLFEEVFTASDGSLYTLSVDQTISHYKQNGELDFQIKDAFGQYASTTDSPTLTV
                     DEDGQMYLLGINSGWVLKFSADGTFLNKFNAADSLKVNPSAPAGDSAFFGLVTPTKTP
                     TPKNILLQSIAVDANSRVYVCTSEGISIFSTSNQYVGSFRPASGNCEKMAFDNASHLY
                     ILTTENRVGKFEILTSTQ"
     gene            complement(1843..3378)
                     /gene="hutH"
                     /locus_tag="PL000_18870"
     CDS             complement(1843..3378)
                     /gene="hutH"
                     /locus_tag="PL000_18870"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNC91002.1"
                     /translation="METIYLDGNSLTFEQVQAVAYGKPNAPRVEISDTAKKLVTRAAD
                     AVQTLLSRGTIAYGITTGFGAFKDRIIPPDQVEQLQRNILVSHAVGVGEPFDIPTTRA
                     IMLIRANTLSRGHSGIRLTTLQLLVEMLNAGVHPLIPEKGSLGASGDLAPLAHMALPI
                     IGEGKVEYQGAVMAGAEAFKRAGLTPIKLAAKEGLALTNGTTIMTAMGVLQTLRAERY
                     SQTADIAGCLSLEALNGTDLAFDERIHNLRPFPRQIECARYLRGLLKDSQFTREHASL
                     NVQDAYTLRCIPQVHGAVRDAIAYARWVFEIELNAVTDNPLIFIDEKEEITVLSGGNF
                     HGEPLAIAMDYLAFSVAELGNISERRIMRLTDESSNIHTLPAFLTKEGGLNSGFMILQ
                     YTAAALATENKVFAHPASVDTIPTSANVEDHVSMGATAAIKLRSVLDNVEKILSIELM
                     SAAQGVDFRKQVIGLEKNLGVGTRGVYKLIRTHIPFIESDTILYEHMETMRQLVAEGR
                     VLA"
     gene            complement(3391..5541)
                     /locus_tag="PL000_18875"
     CDS             complement(3391..5541)
                     /locus_tag="PL000_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HNC91003.1"
                     /translation="MKQLLQNIKNGNTTVEDIPIPTPREGQALIKVAASLVSAGTERM
                     VVEFAEKSYLGKARSRPDLVKQTLDKAKREGIMPTVNAVFNRLDQPMALGYSTAGTIV
                     ALGKNMQGFKVGQRVACAGGGYATHAEYNVVPRNLLTPLPKNVDFESAAFTTLGAIAL
                     HGLRLAEPQLGENVAVIGLGLLGLMTIQLAAAAGCNVLGIDLDPKRIKLASSLGLEAV
                     SRQNAESASAARFATANRGFDSIIICADTPSNDPVELAGVIARDRAKVVATGAVGLNF
                     PRKIYYEKEISFINSRSYGPGRYDSNYEENGNDYPIGYIRWTEGRNFQAVVDLMASGK
                     LKVTPLISHRFDIIEGVKAYEVITGKRKEPFLGVVLKYPEEKMKEERKIVFPSIAHRS
                     SSVVKLGVLGAGLYANATLLPVLKDNKDYELIGIASSGGLHAQHSGKKFGFQYATSSE
                     DEIINDPKINTVAILTRHDTHADLAIKALKAGKHVFVEKPLAINSNQLSVVSKQLLKT
                     DHCLLTTGFNRRFSPLARSLKSQLESLQEPKHLHYRVNAGFIPANHWTQDEAIGGGRI
                     IGEACHFIDTLTFLVGALPVKVSAQALPNNGKYREDNVSMTFTFADGSIGVVDYLANG
                     DKSMPKERLEVFCGGMVAVLDDYVSLSVVKDGKRKEEKGAQDKGWRGEMAAFAEAVQG
                     KGEAPIPYGQIIAVTKSTFAAVESIRSGNPVEIK"
     gene            5756..7582
                     /locus_tag="PL000_18880"
     CDS             5756..7582
                     /locus_tag="PL000_18880"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNC91004.1"
                     /translation="MELGGYANKVAWVDLSAGKVEFKPIAEDDARKYIGGRGLGVKYV
                     FDNGPTVDALSPDNILCFMNGPLTGSEANMSGRMAVVTKSPLTGTVTDSHHGGWSAAR
                     LRWAGYDGLVFKGKAAKPTYIYIHDGQFELLDASEVWGKGVHDTVKHFKEKYGEKDLT
                     VITIGQAGENQVKYACWVNENDRASGRGGTGAVGGSKNLKAIVIKAEKKIVKAANRDK
                     WKEAHTKALAEIMDERVVTSPRKGGLSVYGTNVLMNMTNVIGGLPTKNSQFTGWEHAE
                     KVSGEYVNDNILVDNPTCHACPVACKKEVEIKDGPYAGLRMESVEYEPAWSVGTNCGN
                     DDIRVVAKMIDCANDYGFDAIEVGHPLSVWMEASERGYTNGSGKLNWGDGAAMVAAAE
                     MIAYRKGFGDVLANGANAVAAHFGHPELAMTVKGQGVPAYDPRGIKGMGLGYATSNRG
                     ACHLRGYTPAAEVVGNVLGPSTVADPLAWKGKGEMNFIFQNVHAVTDCLDICKFATFA
                     ESLDSFAMQYEAMTGVPCDAGHLLKVGERVYNLERHINNLNGFREGSDTLPKRFTDEP
                     STYGGSKGQVCELDDMLVEYYEKRGWNNGVVPESKLKELEII"
BASE COUNT         1716 a         2023 c         2016 g         1852 t
ORIGIN      
        1 tggacgagga caacgaatcc ctcgcgcgcc agggcgttga cgtatgccct tccgccgtag
       61 ggagttttgc gaaactcgtt caacacaggc gaaggttctt ctggtccgtc cgcgatcttt
      121 tctttgccgt aaaacttgaa gtcgctatga tcgtgcaacg ccacaatgcc cggaagagtc
      181 cgcgtggcat tggcgggttt gaaaacatac gcatgcgtcc gcggaccaaa ccccaccgac
      241 cacgaaacct cctgcccgat caaaccatcc ttttgccact cgcgctcaat gcggacttcg
      301 gcgggagatt cgtcggtgtg gcaaaagccc agagtttcac gcaccagttc ccgggtccgc
      361 gggcccgggg gcgtgttttg gtaaaggggc tgttgtcgct gtgcggcggc gacccagtcg
      421 ctgaaaattc ccaggtggcg atattgtccc gtcatgtgat gtgttcccgt ttattgtgtg
      481 gaggtcagga tttcgaattt ccccacccga ttttcggtag tgaggatgta caaatggctt
      541 gcgttgtcaa aagccatttt ttcgcagttc ccggaagcgg gtctgaacga accgacatat
      601 tggttgctgg tgctgaaaat ggagattccc tccgaggtgc aaacataaac acggctgttg
      661 gcatccacgg caatggattg cagaaggatg ttcttggggg tcggggtttt ggtgggggtg
      721 actaaaccaa aaaatgcaga atcacctgca ggggcagaag gattgacctt caatgagtcg
      781 gcggcattga atttgttgag gaaggttcca tcggctgaaa acttgagcac ccatccgctg
      841 tttattccaa gcagatacat ctgtccgtcc tcatccacgg tcagggtggg ggagtcggtg
      901 gtgctggcgt attgaccaaa ggcatccttg atctgaaaat ccagttcccc gttttgtttg
      961 tagtggctga tggtctggtc aacagatagg gtgtacagcg acccatcaga agcggtgaag
     1021 acctcttcaa aaagttggtc tttcatcacg cctgtttcct gaccttccgt gttgtagcgg
     1081 tgaatttcca gattgtgtac aagcaggatc tctccactcg tggaaacggc catcccagtg
     1141 acgggggcat ttttgttggt ggctatggtt gtgatgagtt ttcccgtttc atcaaagata
     1201 cgaatatggc ctgcatcggt atcactcacc atgatattgc cctgactgtt cacaaccaga
     1261 gcggaggcat ctttggggtt ttcgggggcg ctgtctttca agttaaaggt caacaggtgt
     1321 ttcgccaggc ttggcggacg gggtgtgctt gtagcggctg gggtattatt gggccgcagg
     1381 gttggaacag gacgataggc aggctttgag gaacggatca cccccaagac gacgaagaaa
     1441 atagccgcaa caatggcgat gatgatttgc cacgacctct taatatcctg ttcggcggca
     1501 tcaagttcag catcctctgg gtctactatg gttttacttt cggtttgtgc aggcggtgtg
     1561 acaggtgttg cagactgggc gctttgtgaa atattcacca gtgtgccgca atatccgcac
     1621 ttgacgcgtt tggcttcttc tttggcttcg agcggcgccc cacaggatgg gcaaatgatg
     1681 gctttggatt ctttgttgct catgttctct ctcttgggtg aaatttttac ctaggttaat
     1741 gcacaggttc cactacgtcg ataggaaatc gtggggaatt gagaaagcaa aaggccgagg
     1801 cgccaacctc gaccttttgc tttcggttct gtgaaaaacg ggttacgcca aaacccttcc
     1861 ttccgccacc aattgacgca tcgtttccat atgctcatac aggatcgtgt cagattcaat
     1921 aaaagggatg tgagtacgga tcaatttgta gacgcctctt gtccccacgc ccaaattttt
     1981 ctcaaggccg atgacctgtt tgcggaagtc cacgccctgc gcggcagaca tcaactcgat
     2041 ggagaggatt ttctccacgt tatcaagcac gcttcgcaac ttaattgcag cggttgctcc
     2101 catactgaca tggtcctcca cgtttgcaga agtggggatc gtatccacac tggcagggtg
     2161 tgcaaagacc ttgttctcgg ttgccaatgc cgccgctgtg tattgcaaga tcataaagcc
     2221 agaattcaat ccgccttctt tggtgaggaa cgcaggcagg gtgtggatgt tcgaactttc
     2281 atcggtcagg cgcatgatgc ggcgctctga aatattgccc aactccgcca ccgaaaaagc
     2341 cagataatcc atcgcaattg ccagcggttc accgtggaaa tttccgccag ataaaaccgt
     2401 gatctcctcc ttttcatcaa tgaagatcag cggattatcc gtcacggcat tcagttcgat
     2461 ctcaaaaacc cagcgtgcat aggcaatggc atcacgaacc gccccatgca cctgtggaat
     2521 gcagcgcaag gtgtacgcat cctgcacatt caacgaagca tgttcacggg tgaattggct
     2581 gtctttcagc aaaccccgca aataacgcgc acactcgatc tggcgcggaa aaggccgcag
     2641 gttgtggatc cgctcatcga aggcgaggtc ggtaccgttc aatgcttcga ggcttaagca
     2701 gcctgcaatg tcagcggttt ggctgtagcg ttctgcgcgc agtgtttgca gcacgcccat
     2761 cgctgtcatg atggtcgtgc cattcgtcag tgccagacct tctttcgcag ccagtttgat
     2821 cggcgtcaga cccgcacgtt tgaatgcctc agcgccagcc atcactgcac cctgatattc
     2881 caccttcccc tcgccgatga tcggcaaagc catgtgcgcc agcggagcaa ggtcaccgct
     2941 tgcgccgagc gatccctttt ctgggatcag cggatgcacc cccgcattga gcatctccac
     3001 cagcaattgc aaagttgtga gtcgaatacc ggaatgtccg cgcgagagtg tgttggcgcg
     3061 tatcagcatg atggcccgcg tggttgggat atcgaacggt tcgccgacac ccacggcatg
     3121 gctcaccaag atattgcgct gcagttgctc cacctggtcg ggtggaatga tgcggtcttt
     3181 gaacgccccg aagcctgtgg tgattccata cgcgatcgtg ccgcgtgaaa gcagggtctg
     3241 cacggcatcg gcggcacgcg tcaccagttt ctttgccgta tcgctgatct ccactcgcgg
     3301 cgcatttggc tttccataag ccactgcctg cacctgttca aatgttaaac tgtttccatc
     3361 cagatagatc gtttccatgc aacctcgagg ttattttatt tcgacaggat ttccacttcg
     3421 aatcgattcc accgccgcaa atgtggattt ggtcacagcg atgatctgcc catagggaat
     3481 cggcgcttcg cccttccctt gaactgcttc agcaaaagcg gccatttctc cccgccagcc
     3541 tttgtcttgt gccccttttt cttccttcct ttttccgtct ttcaccacac tcaatgagac
     3601 ataatcatcc agaaccgcca ccatgccccc gcaaaagact tcaagccgct ccttcggcat
     3661 ggatttatcg ccattggcaa ggtaatccac cacgccgatg gaaccatccg caaaagtgaa
     3721 ggtcatcgag acattgtcct cgcggtattt gccgttgttg ggcaaagcct gcgccgaaac
     3781 tttcacaggc aaagcaccga ccaaaaaagt gagcgtgtca ataaaatgac aggcttcacc
     3841 gatgatgcgt ccgccgccga tggcttcgtc ctgagtccaa tggttggcag ggataaaacc
     3901 agcgttgacg cggtagtgca ggtgtttggg ttcctgaagg ctttcaagtt gagatttgag
     3961 cgaccgggca aggggggaaa atcttctatt aaacccagtc gtcagtaagc agtgatcagt
     4021 tttcagcaac tgtttactga caactgacaa ctgattacta tttatcgcca acggcttttc
     4081 cacaaacaca tgcttcccag ccttcaaagc ctttatggcg agatcggcat gggtgtcgtg
     4141 tctggtgagg atggcaacgg tgttgatttt tgggtcgttg atgatctcgt cttctgaaga
     4201 tgtggcatat tggaaaccaa actttttgcc tgagtgctgg gcatgcaaac cacctgaaga
     4261 ggcaatgccg atcagttcat aatctttatt gtcttttagc actggcagca gggtggcgtt
     4321 ggcataaagc cctgcgccaa gaacacccag cttgaccaca gacgatgaac gatgggcgat
     4381 ggaaggaaat acaatttttc tttcctcctt catcttttct tcagggtatt tcaacaccac
     4441 cccaaggaaa ggctcttttc tctttcctgt aatgacttcg tacgctttga ccccttcgat
     4501 aatatcgaag cgatggctga tgagcggtgt cactttcaac tttccgcttg ccatcagatc
     4561 cactactgcc tgaaaatttc gtccttccgt ccagcggatg tagccgatgg ggtagtcgtt
     4621 gccgttctct tcgtaattcg agtcgtagcg gcccgggccg taggagcggg agttaatgaa
     4681 ggagatttct ttttcgtagt agatctttct ggggaaattc aggccaactg ctcccgtggc
     4741 taccactttg gcacggtctc gagcgatgac tcccgccagt tccacaggat cgttggaggg
     4801 agtgtcggcg cagatgatga tgctgtcgaa tccgcggttg gcagtcgcga agcgtgccgc
     4861 agatgctgat tctgcatttt ggcgggagac agcttccagt ccgagtgaag aggcgagttt
     4921 gattcgcttt gggtcaaggt caatgccaag gacgttgcat cctgctgcgg ccgccagttg
     4981 aatcgtcatt aatcccaaaa gacccaggcc gatgacagcg acattttctc cgagttgcgg
     5041 ctctgccagt cggaggccgt gcaaggcaat ggcaccaagg gtggtgaagg cagcggattc
     5101 aaaatcgaca tttttgggga gtggcgtgag caaattgcgc gggacaacat tgtattcggc
     5161 atgggtggca tacccgccgc ccgcacacgc aacccgctga ccgactttga agccctgcat
     5221 atttttgccg agtgccacaa tggttccagc agtggagtag ccaagtgcca tcggttgatc
     5281 gaggcgattg aacaccgcgt tgaccgtggg catgatacct tcgcgtttgg ctttatcgag
     5341 ggtctgcttc acgaggtcag gacgagagcg tgccttgccg agatagctct tttctgcaaa
     5401 ttccaccacc atgcgttcgg ttcctgctga aacaaggctt gctgccacct tgatcagagc
     5461 ctgcccttca cgtggggtgg ggatggggat atcttctact gtggtatttc catttttgat
     5521 gttttgaagg agttgtttca tggatgagct ttctatagtt ttaccaagta ttgaaagtat
     5581 aacgcaattc aaaatgcgtg gacttgatgt gaaaaataac gcaaaggaag aatgtcacct
     5641 ttaccgttaa gtttttactt aattattgga atcgatttca ccaaatgcta ttctgaacgc
     5701 aggacggacg gcttgcgccg ttctatattc atttgtcaaa aaaaggagat tcattatgga
     5761 actcggtggt tatgcaaaca aggttgcttg ggtagacctt tccgctggta aagtggaatt
     5821 caaacccatc gccgaagacg atgcccgcaa atacatcggc ggacgcggtt tgggcgtgaa
     5881 gtatgttttc gacaacggcc ccacggtcga tgccctctcc cccgataata tcctctgctt
     5941 catgaacggc ccgctcacag gctctgaagc caacatgagt ggacgcatgg ctgttgtgac
     6001 caaatcgcct ctcactggaa ccgtaactga ttctcaccat ggtggctggt ctgctgcccg
     6061 tttacgctgg gctggatatg atggcttggt cttcaaaggc aaggctgcca aacccaccta
     6121 tatttatatt catgatggcc agttcgaact gcttgatgcc tctgaagttt ggggtaaggg
     6181 cgttcacgac accgtgaagc acttcaaaga aaaatatggt gaaaaagacc tcaccgtcat
     6241 taccattggt caggctggtg aaaaccaggt caagtatgcc tgctgggtta atgaaaatga
     6301 ccgcgcttcc ggacgcggcg gcaccggcgc agttggtggc tcaaagaacc tcaaagccat
     6361 tgtcatcaag gctgagaaga agatcgtcaa agccgcgaat cgcgataagt ggaaagaagc
     6421 ccacaccaaa gccctcgccg aaatcatgga cgagcgcgtt gttacctctc cccgcaaggg
     6481 tggtctctct gtttatggca ccaacgtgct catgaacatg accaacgtca tcggcggtct
     6541 gcccaccaag aacagccagt tcaccggttg ggaacacgct gaaaaagtca gcggcgaata
     6601 tgtcaatgac aacatcctcg tagataaccc cacctgtcac gcctgccccg ttgcatgcaa
     6661 gaaggaagtt gaaattaaag atggtccgta cgctggtctc cgcatggaat cagtggaata
     6721 cgaacccgcc tggtcggtcg gcacaaactg cggcaatgac gatatccgcg ttgtcgccaa
     6781 gatgatcgac tgcgccaacg actatggctt cgatgccatc gaagttggtc acccgctctc
     6841 cgtttggatg gaagccagcg aacgcggcta caccaacggc tctggcaaac tcaactgggg
     6901 cgacggcgcc gccatggttg ctgccgctga aatgatcgcc taccgcaagg gttttggcga
     6961 tgttctcgcc aacggcgcga atgcagttgc tgctcacttt ggccaccccg aactcgccat
     7021 gaccgttaag ggtcagggcg tgcccgcata cgatccgcgc ggcattaagg gcatgggcct
     7081 gggctacgcc acatccaacc gcggtgcctg ccacctgcgc ggctacaccc ccgccgccga
     7141 agtcgtcggc aacgtacttg gaccttcaac cgttgctgat ccgctcgcgt ggaagggcaa
     7201 aggcgaaatg aacttcatct tccagaacgt tcacgccgtc accgactgcc tcgacatctg
     7261 caaattcgcc accttcgccg aatccctcga ctccttcgcc atgcaatacg aagccatgac
     7321 gggcgtcccc tgtgatgcgg gtcacctgct caaggtcggc gaacgcgttt acaacctcga
     7381 acgccacatc aacaacctca acggcttccg cgaaggctcc gacaccctgc cgaagcgctt
     7441 caccgatgaa ccctccacct acggtggcag caagggtcag gtctgcgaac tcgacgacat
     7501 gctcgtggaa tactacgaga agcgcggatg gaacaacggc gttgtgcccg aatccaaact
     7561 caaggaattg gagatcatct aattccccgc ttcattccta actgaag
//
LOCUS       DAOQLC010000160         7542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3968_length_7542_cov_22.933752, whole genome shotgun sequence.
ACCESSION   DAOQLC010000160 DAOQLC010000000
VERSION     DAOQLC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3968_length_7542_cov_22.933752"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PL000_18885"
     CDS             complement(<1..326)
                     /locus_tag="PL000_18885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91005.1"
                     /translation="MLPTILEGKKIFIIEDDPMSLSLISSCLLKHGARIEANALGFDI
                     IDHLVESMPVDLIILDVNLRKNLPGYQVLENIRSMPSLYQVPVVMVTATDWEIALPKA
                     RAAGANG"
     gene            complement(326..4027)
                     /locus_tag="PL000_18890"
     CDS             complement(326..4027)
                     /locus_tag="PL000_18890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNC91006.1"
                     /translation="MKNQSTMTDKVILRAQALAWAGKHTQAIETASHELGRHNLKPKM
                     QMDLLDLRAESYIAIGKLDLAAADADAMTRLAKQGLSLSIQALNRKAIVQMRTGDLKS
                     AVKSASAALKIAQKSKQKRCISQSLLILSESQTRKGQLEESIVTAEKAIAIFQELNDL
                     SGAGRAYWCISTACFDLFRAENSRKAAQAALELCQQAGDQYGIGNAYNAYTYTDTDIA
                     ERLQHLHQAKAAYETAGYVERQTVALVNLAVVYSELGLYTHNRRLNEEAIRITGTMGA
                     RLLFIYALGNIIDTEIKLGELQSAQRRIKEYVKLVPTLGDPTQETSLAQALGDIAFAE
                     GNFKIAIQHYQSGLRISAQTNLGRENVFLTEISKTHLASRDSATALKTTTKATNLHRE
                     QSYAKPDGFPTQAIWWRHVQALLANKKIKEAREALDRAYDLLLEGIQNIPDVGLRRNY
                     LNKVALNRELLQYWLKDASKRKLSKERTCAYLNIESNPREPFKRLTNTSLRLNALHTV
                     EEIKNFLVEEATELSGGERVVLILENDGKREAANSLLPHGEDARTFPASIGRYLTATR
                     LTHTTQLILPKKQGLSRIIAPLITQNKLIGYIYVDMDSTYGVFNETDRDMIGMLANQA
                     AIAIDNASLVIGLEQKINERTHQLQERIYELQIINSFQQNLAAEMDFQSIVSFVGDKL
                     REVFNTSDLGINWYDEKSNLLHYLYAYEHGVRLNVPPQAPRPGGLFERMTRTRQPFIL
                     NNAADYKKMNVPAIPGTDQSKSSIAVPIISNDRVLGFIATENHQRENAFGESELRLLT
                     TIAASLGSALENSRLLDETQRLLKESNTLADVGRDISSSLETKTVLENINSYASNLLK
                     GSMSAIFIPEKDGDSFRAIAVVGHNAEELRNEVIASGEGVLGDIARNKSAEIVNNIDN
                     DPRAVMITGTENVPDEHLLAVPLLADDELKGLMAVWRAGKNMSFTHNELTFLTNLARQ
                     AVIALKNSQLFTEMQEARATAEQADKAKSAFLANISHELRTPLNAIINFTELVAMGTM
                     GPINIEQEEALGYSLSSSKHLLQLINDVLDISKIQAGKLTLFVENDVNLHVEIQDTLK
                     MVEPLLHKHAELYGHDVKLIRDIDKGLPIIACDRRRIKQVLLNLLSNAVKFTQKGSIT
                     LSAKRHEDHIAFAVMDTGQGISQDQQKQIFEPFTQALEGTKHVEGTGLGLPITKSLIE
                     AHGGSIWLESTPGEGTSFFFKIPTTIQAG"
     gene            complement(4069..4476)
                     /locus_tag="PL000_18895"
     CDS             complement(4069..4476)
                     /locus_tag="PL000_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91007.1"
                     /translation="MIKDPVFLYVEDDPYSIKVMRMIFEKVMRIPTLIVFEGSDNFMQ
                     NLLELNTPPDVFLLDIQMKPYNGFELLHMIRADRRFRASKIVALTASVMGEEVDQLKR
                     SGFDGAISKPIHITSFPGLINRVLNGENIWFVS"
     gene            complement(4508..4930)
                     /locus_tag="PL000_18900"
     CDS             complement(4508..4930)
                     /locus_tag="PL000_18900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91008.1"
                     /translation="MTITSTYHLENKRIFLIEDDVLNLAIINRVLINSKATVFQNYNS
                     IGIVTHIIQHLPIDLILLDIMLRRGVSGMDVILELKNNIHTANIPVVAVSALDPEIAI
                     PQAQRAGFNGFIGKPINSMSFSQDIAAIIHGEQRWVAS"
     gene            complement(5061..5813)
                     /locus_tag="PL000_18905"
     CDS             complement(5061..5813)
                     /locus_tag="PL000_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91009.1"
                     /translation="MENLQKFDSIISLFTKLHQGQHTPPVGEWKRRQVPNPSAKSPAV
                     QILIVDDHRYVHDVISKIMVNVPDMTIAGHAANGQEAIQFCQTHQPDIILMDVVMPVM
                     DGLDASRTIHSLYPEVKILVLSSFQDHESVYALLKNGAVGYITKNSLTSELIQIIRTT
                     ATGKTVFSPEVMEQLLFSSQNPQMIYKFNLTDRELEILVLLADGMLNQQIAKKLTISL
                     STVKFHKTNIFQKLGVKTRSEALVVAVKNNLI"
     gene            complement(5791..6819)
                     /gene="fni"
                     /locus_tag="PL000_18910"
     CDS             complement(5791..6819)
                     /gene="fni"
                     /locus_tag="PL000_18910"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HNC91010.1"
                     /translation="MKKVAPIDQRKADHIKINLEQDVRSALTTGLEKYRFIHEALPEL
                     DLNRIDTTLSLFGKKLASPTLVSSMTGGTSEAETINMRLAEAAQECGIAMGVGSQRAA
                     IEHPEQAATFQVRRVAPDILLFANIGAVQFNYGYGIDQCRKAIEMIEADGLYLHLNPV
                     QEAVQDAGDVNWVGLAKKIEEVCKKLEVPVIAKEVGWGISEHTARILADCGVQAIDVA
                     GAGGTSWSQVEMHRAPDEFTRQLAATFVGWGIPTAESIVGVKRAVPEMTIFASGGIKD
                     GLDIAKCVALGASLGGMAGHFLKAAAISTENVIETMELVKRQIEVTMFATGAGTLASL
                     RNGKLAKV"
     gene            6916..7158
                     /gene="acpP"
                     /locus_tag="PL000_18915"
     CDS             6916..7158
                     /gene="acpP"
                     /locus_tag="PL000_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC91011.1"
                     /translation="MSDTFSELKDIIKDLLGADEAKITPEARFREELEADSLDLVELI
                     MAFEDKFGTEISDEDAQKITTVGEAVAYIDAHRGSK"
     gene            complement(7253..>7542)
                     /locus_tag="PL000_18920"
     CDS             complement(7253..>7542)
                     /locus_tag="PL000_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC91012.1"
                     /translation="VLEMFEHDPKTEKVIMIGEIGGSEEEKAAEYIAKKMTKPVAAFI
                     AGQTAPPGKRMGHAGAIIEGGAGTAADKIKALEAAGVKVAKHPEEIPTLLQ"
BASE COUNT         1695 a         1683 c         1972 g         2192 t
ORIGIN      
        1 ccgtttgcgc ctgctgccct tgcctttgga agtgcaattt cccaatcggt ggcagtgacc
       61 atcacaactg gcacttgata aagcgacggc atggagcgga tattctccaa aacctgatac
      121 ccaggcaggt ttttgcggag attgacatct aaaatgatca agtccacagg catggattcg
      181 acgaggtggt cgataatgtc aaaccctaat gcattggctt cgatacgggc tccatgttta
      241 agcaggcagg aactgatcaa actaaggctc atcgggtcat cttcgatgat gaagattttt
      301 tttccttcaa gaatggtggg caacatcaac cagcctgtat ggtggtgggg attttgaaga
      361 aaaatgatgt cccttcacct ggtgtgcttt cgagccagat acttccacca tgcgcctcaa
      421 tcaggctttt ggtaatcggc aaccccaggc cagtgccttc cacgtgtttg gtcccttcca
      481 aagcctgggt aaaaggttcg aagatctgct tctgttggtc ttgcgaaata ccctgacctg
      541 tatccatgac agcgaaggca atatggtctt catgtcgttt ggcacttaat gtgatgcttc
      601 ctttttgcgt gaacttcacc gcattggaaa gcaggttaag taacacttgt ttgattcggc
      661 ggcgatcgca ggcaatgatc ggcaggcctt tatctatatc gcggatcagc ttcacgtcat
      721 gtccatatag ttctgcgtgt ttgtgcagca agggctcaac catctttaat gtgtcttgaa
      781 tttcgacatg gaggtttaca tcgttctcaa cgaagagggt gagtttgcca gcctgtatct
      841 tgctgatatc cagaacatcg ttgatcaatt gcaatagatg tttcgaactg ctcaaggaat
      901 aaccaagagc ctcctcttgc tcaatgttga tcgggcccat tgtgcccatt gcaaccagtt
      961 cagtgaagtt gataatggca ttcagcggag tgcgtagttc atggctgatg ttggccagaa
     1021 aagcgctttt ggctttatca gcttgttctg ctgtggcgcg ggcttcctgc atttcagtga
     1081 acaactgcga gtttttcaat gcaatgaccg cctgccttgc cagattggta aggaaggtaa
     1141 gttcattatg agtgaaactc atatttttac ccgcccgcca cacggccatc aggcctttca
     1201 attcatcatc agccaacagt gggacagcca gcaggtgctc atcggggaca ttttccgtgc
     1261 ctgtaatcat caccgcacgt ggatcattgt ctatattatt tacgatctca gcagatttat
     1321 ttctggcaat atcgcccaat acaccttccc ccgatgcgat aacctcattt cgaagttctt
     1381 cggcattgtg ccccactaca gcaattgcgc ggaagctatc cccatctttt tctgggatga
     1441 agatcgcgct catgcttccc ttgagcaggt tactggcata cgagttaatg ttctcaagga
     1501 cggttttggt ctcaaggctg gaggaaatgt ctcggcctac atcggcgagg gtattgcttt
     1561 ccttcaacag ccgctgggtt tcatcaagca gacgagagtt ttcgagggca gaaccaagcg
     1621 aggcagcgat cgtggtcagc aggcgcagtt cagattctcc aaaggcattt tcacgctgat
     1681 ggttttcggt ggctatgaat ccaagcacac ggtcattgct gatgatgggc accgcaatgc
     1741 tggacttgct ttggtcagtc cctggtatgg cgggtacatt catctttttg tagtctgctg
     1801 cattgttgag gatgaacggt tggcgggtgc gggtcatcct ttcaaacagg ccgcctgggc
     1861 gtggagcctg cggaggaaca ttcaacctga cgccgtgttc ataggcgtat aaatagtgga
     1921 gcaggttcga tttttcatcg taccaattga ttccaagatc ggaggtgttg aacacttctc
     1981 taagtttgtc gcccacgaaa ctcacgatgg attggaaatc catttcagcg gccagatttt
     2041 gctggaagga gttgatgatt tgcagttcat agatgcgttc ctgcagttga tgggtgcgct
     2101 cgttgatctt ttgttcaagg ccaatgacga ggcttgcatt atctattgcg atggctgcct
     2161 gatttgcaag catgccgatc atatcgcggt cggtttcgtt gaagacaccg taggtcgaat
     2221 ccatatccac gtagatgtag ccgatgagtt tgttttgggt gatgagtggg gcaatgattc
     2281 ggctgagtcc ctgctttttg gggagaatca gttgagtggt atgggtgagg cgggtagcgg
     2341 tgagatatct gccgatcgaa gcggggaatg tccgcgcgtc ttctccgtgt gggagtaggg
     2401 aattcgctgc ttcgcgcttt ccgtcgtttt caaggattaa gacaacacgt tctccaccgc
     2461 tgagttcggt tgcttcttcc acaagaaaat ttttgatctc ttcaacagta tgtaaagcat
     2521 taagacgcag gctggtattg gtgagacgtt tgaacggttc gcggggattc gattcgatat
     2581 taaggtaggc gcaagtacgt tcttttgaga gttttcgctt gctggcatct ttcagccaat
     2641 attgcagcaa ttcacgattg agcgccactt tgttgaggta gttacgacgg aggcctacat
     2701 ctggaatatt ttgaatacct tccagcaaca ggtcataagc gcggtcaagc gcttcacgag
     2761 cctcttttat ttttttgttg gcaagcagtg cctgcacatg acgccaccag atcgcctgtg
     2821 tggggaaacc gtctggtttg gcataggatt gctcacgatg caggtttgtg gcttttgtgg
     2881 tggttttgag cgctgtggcg gagtctctgc ttgcaaggtg ggttttgctg atttcggtga
     2941 ggaaaacatt ttccctgccc aaatttgttt gtgcagagat tctgagtcca gattgatagt
     3001 gttgaatggc tatcttgaaa ttgccttccg caaaggcgat atctcccaga gcttgggcaa
     3061 ggcttgtttc ctgtgttggg tcgccaagtg tggggacgag tttcacgtat tcttttatgc
     3121 gtcgttgggc agattggagt tcgccaagtt tgatttctgt gtcaattata ttgccaagtg
     3181 cgtaaatgaa taataatctg gctcccatgg tgccagtaat gcggatcgct tcttcattca
     3241 ggcggcgatt gtgtgtatac aggccaagct cggaatatac aacggcaagg tttacaagag
     3301 caacagtttg gcgttctaca tagcctgcgg tttcgtaggc agctttggct tgatgcaggt
     3361 gttgaaggcg ttcggcaata tctgtatcgg tataggtgta ggcattgtat gcgttgccga
     3421 tgccgtattg atcacccgcc tgttgacaga gttcaagcgc ggcttgtgcg gctttgcgtg
     3481 aattttcagc gcggaacagg tcgaagcagg cagttgagat gcaccaatat gcgcggcctg
     3541 cccctgaaag gtcattgagt tcctgaaaaa tggcaatggc tttttctgcg gtaacaattg
     3601 attcttccag ttgtcctttt cgagtttgag attcgctgag gatgagcagg ctttgagaaa
     3661 tacacctttt ctgttttgat ttttgggcaa ttttcaatgc ggcactggca gacttgacag
     3721 cacttttcag gtcaccagtg cgcatttgaa cgatggcttt gcggttgagt gcttgaatgg
     3781 acaaggataa accctgcttt gccagccgtg tcatggcatc tgcatctgcg gcggcgaggt
     3841 cgagttttcc aatggcaatg taagactcgg ctcgcaggtc gagcagatcc atttgcattt
     3901 tgggtttcag gttgtgcctg cccaactcat gcgaggctgt ttcgatggct tgtgtatgtt
     3961 tgcccgccca tgccagagct tgcgcacgca aaataacttt atcggtcatt gttgactgat
     4021 ttttcatgac ttcatccaat ttctgtaaat caggaagatg tgaacttttt atgaaacgaa
     4081 ccagatgttt tcaccattca ggacgcggtt gatcagacct ggaaacgatg tgatatgtat
     4141 gggttttgaa atggcaccat cgaatccgct tcttttgagc tggtccactt cctcgcccat
     4201 gacacttgcg gtgagtgcca caattttact tgctcgaaac cgtctgtctg cgcgaatcat
     4261 gtgtaaaagt tcgaagccat tgtatggctt catttgaata tccagcagga aaacatcagg
     4321 aggcgtattt aactcaagca ggttttgcat gaaattatca ctgccttcaa aaacgatcaa
     4381 ggttggaatg cgcatcactt tttcgaagat cattctcata accttgatgg aatatgggtc
     4441 gtcttccaca tacagaaata cagggtcttt gatcatgaat gttgtattcc tgaggccgcc
     4501 cgaccagcta gctggcaacc catcgctgtt ctccatgaat aatggcggcg atatcttgcg
     4561 agaaagacat tgaattaatt ggctttccga tgaatccgtt aaaaccagct ctttgtgcct
     4621 gcgggattgc tatctccggg tcaagcgcag atacagctac aacagggata ttggcggtgt
     4681 gtatgttatt tttgagttcg agtattacat ccatgccgct gacgcccctg cgcagcatga
     4741 tatcaagcag gatcaggtca atgggcaggt gttgaatgat gtgggtgaca ataccgatgc
     4801 tgttgtagtt ttgaaatacc gtggccttac tgttgattag cacgcgatta atgatcgcca
     4861 gattcaaaac atcatcctcg atcaggaaga tccttttgtt ttcaagatgg taggtgctgg
     4921 tgatggtcat aattctcctt tgctgtttcg ctgttggttt caaaaccctg atttgtcagg
     4981 caactgcgcg attttattgg caggcaggga gattgggtac tgtctcttta aaacgaaaac
     5041 ctgtccattt ggacaggtag ctagatgagg ttgttcttga cggcgaccac cagtgcctcg
     5101 gagcgtgttt ttacacctaa tttctggaaa atgtttgttt tatgaaactt cacagtggaa
     5161 aggctgatgg taagtttttt cgcgatctgc tgattgagca tcccatctgc cagaagaacg
     5221 aggatttcga gttcacggtc tgttaggttg aatttgtaga tcatctgcgg gttttgggaa
     5281 gaaaaaagca attgttccat cacctctggg gaaaataccg tcttcccagt tgcggttgtt
     5341 ctaataattt ggatcagctc actggtaagg gagtttttag tgatgtatcc aactgctccg
     5401 ttttttagga gagcatatac actttcatga tcttgaaaac tggaaagcac aaggatcttg
     5461 acctcagggt aaaggctgtg aatggtgcga gatgcgtcga gaccatccat gacaggcatg
     5521 acaacgtcca tcaatataat gtcaggttgg tgggtctgac agaattgaat ggcttcctgc
     5581 ccgttggcag catgacctgc aatggtcatg tctgggacat tgaccatgat ttttgaaata
     5641 acatcgtgaa catatctgtg atcgtccacg attagtattt ggactgccgg ggattttgct
     5701 gaagggttag gcacctgtct tcttttccat tctcctactg gtggtgtgtg ctgtccctga
     5761 tgtaattttg tgaacagtga aattatacta tcaaactttt gcaagttttc cattgcgcaa
     5821 tgatgccaat gttcctgctc ctgtggcaaa catggtgacc tcgatctgcc ttttgaccag
     5881 ttccatggtt tcgatgacat tctcagtgga gatggccgct gccttgagga agtgccctgc
     5941 catgccgccc agggatgcac cgagggcaac acatttggcg atatcgagac cgtctttgat
     6001 gccgccactg gcgaagatgg tcatttcggg cacggctctt ttcacgccga caattgattc
     6061 tgctgtggga attccccacc cgacaaaagt cgctgccagt tggcgggtga attcatcggg
     6121 tgcgcgatgc atttcgacct gagaccagct tgtgccgcct gctccagcca cgtcaatggc
     6181 ttgtacgccg caatcggcca aaatcctggc tgtgtgttct gaaattcccc aacccacttc
     6241 cttggctata acgggtacct caagtttttt gcaaacctct tcgattttct ttgcgagacc
     6301 aacccaattg acatctcctg catcttgtac ggcttcctgc acagggttta agtgcagata
     6361 aagcccatct gcttcgatca tctcgatggc tttgcggcat tggtcaatgc cataaccgta
     6421 gttgaattgc acggcgccga tattggcaaa gagcagaata tccggtgcaa cacggcgtac
     6481 ctgaaatgtg gcagcctgtt ccgggtgttc aattgcagcg cgttgacttc caacccccat
     6541 ggcaattcca cattcctggg cggcttcagc gaggcgcatg ttgatggtct ctgcttcaga
     6601 agtgccgcct gtcatggaac tgaccagggt gggggatgcc aattttttgc cgaacaggct
     6661 gagggtcgtg tcgatgcggt tcaaatccag ttctggcagg gcttcgtgaa tgaatcggta
     6721 cttttcaagt ccagtggtca gtgcagagcg aacatcctgt tctaagttga ttttaatgtg
     6781 gtcagctttt ctttgatcaa tgggcgcaac ttttttcata aggtagtgtt cctgagtatg
     6841 atgatccgaa cccggcttga catgtcaagt ggacggatta caataggcca gtcaaaattt
     6901 gaacaggagg ttgtcatgag tgacacattt agtgaactga aagatatcat caaggatctg
     6961 cttggtgcgg atgaggcgaa gatcacacct gaagcccgtt tccgcgaaga attggaagcc
     7021 gattcgctcg atctggtcga gttgatcatg gcgtttgaag ataaatttgg caccgaaatt
     7081 tccgatgagg atgcccagaa gatcaccact gtgggtgagg ctgttgcata cattgatgct
     7141 caccgcggca gcaagtagaa actccgcaat tactgaatga gattataacc ttagagccgc
     7201 ccgacgacgt ggtcgccagg cggctctatt gattcttgat agagattgaa agctattgca
     7261 gaagggtggg aatctcttcc gggtgcttgg caaccttgac gccagcggct tcaagggctt
     7321 tgattttgtc ggcggctgtg cctgctccgc cttcgatgat tgcaccggca tggcccatgc
     7381 gttttccggg aggggcggtt tgcccggcga tgaaggctgc aacgggtttg gtcatcttct
     7441 ttgcgatgta ttctgcggct ttttcttctt cgctaccgcc aatttcgccg atcatgatga
     7501 ccttttcggt cttggggtca tgttcgaaca tttcaagcac at
//
LOCUS       DAOQLC010000161         7528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_3976_length_7528_cov_24.256122, whole genome shotgun sequence.
ACCESSION   DAOQLC010000161 DAOQLC010000000
VERSION     DAOQLC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7528
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3976_length_7528_cov_24.256122"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /locus_tag="PL000_18925"
     CDS             complement(<1..1674)
                     /locus_tag="PL000_18925"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC91013.1"
                     /translation="MADYLFRGSLEKLDPETYELTQLEQERQYRKLILIPSESTAPMA
                     TREALASAFQNIYAEGYPDEESRWMTEDEILDYPARLADYRRNGDPRYYKGVEYADAV
                     EALARRRAAETFAANGFSADQIYVNVQALSGGPANNAVYQALLNVGDTVMGLDLLHGG
                     HLSHGSPVNRSGKWFKAVHYSVDANEKLDYEAIRQLALENKPKLLIAGYSSYSWIPDW
                     KKFREIANEVGAYFLADISHIGGLVAAGVVPSPIGIADVVMSTTHKSLDGPRGAVLMT
                     TKADVAKKIDKAVFPGEQGGPHVNVFAGLALTFKLAQTKQFKRLQEQTIKNAAAMADQ
                     FTKRGLRVPFGGTNCHMLNVDCSSIKGEDGTTLSGDQAARILDIVGVVVNRNTIPGDK
                     SAAAPSGIRLGTPWITQRGFDEKKSRQLADVIADVLLACAPHSVDTVRKGKQRRAKLD
                     FNVLNAARLKVRKLAEGAGIDFKSKKSGYPHFYYIDDKSTSGVFEITGLRVRQVFDYA
                     ASSDLSALKPGASQATTIATPKGTVKGVLTCIDFGAYYLQVPAAKAGVVG"
     gene            1858..2331
                     /locus_tag="PL000_18930"
     CDS             1858..2331
                     /locus_tag="PL000_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91014.1"
                     /translation="MSNIPRIMRAVGWLLFMVMWIPFTLMFLNMPNGNSNYDSLIGLP
                     KTMALFLGITIGLAIISTALLVGSSVVGWLIGRMVLARGESGTARIIAIQPTGTRVNN
                     YYYGMRFTLEVQSFGEAFQSHAERLVPMHDMTKYQIGMTVNVKYDPMTKTVAMVD"
     gene            2683..3168
                     /locus_tag="PL000_18935"
     CDS             2683..3168
                     /locus_tag="PL000_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91015.1"
                     /translation="MKKKISILIGGLLAVAMLFGAVSVSTAYAQSGSLTPFSDHGGPG
                     HGGRGLGQAELEAAAKVLNMTADELSTALQAGKTLEELATAAGVDIQDVKDAIMAVHA
                     EEIRARIEAGVADGSISQEKADWLLEGLDKGFLDGPGFGFGPGGHHDGQPAQPAQTTT
                     P"
     gene            complement(3254..4018)
                     /locus_tag="PL000_18940"
     CDS             complement(3254..4018)
                     /locus_tag="PL000_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91016.1"
                     /translation="MNSSTESMRWETKVFIFLMISSCLFTTGICACFSSPLILADEDV
                     REELQQFGFSGPAEKCPQIPTGWVIAKENTFDKNLNLWPLGKNSDDYDITNLGMADGV
                     LHYEVEALQGVYSRQFPGLDKNLWDFYLTAQIQKVSGPRDARYGVIFRVRGEKQLFFS
                     IRDSGSVRVAVHEYGGWLDPLFTGYSRYVNAEESNELVVIAQGDIYTFCVNGYIVGSV
                     KTTKYMSGKFGLGFELNGGEKAIFEIDNFNTYAPEE"
     gene            complement(4147..5277)
                     /locus_tag="PL000_18945"
     CDS             complement(4147..5277)
                     /locus_tag="PL000_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91017.1"
                     /translation="MKNKLQIIISVILLMAVTLACGGTAATPAAQDPNIVINTAIAGT
                     QQANALAQATVNANVLTAMPATPTPGPSVDYVALTEEELAALIDEAVAEAVAATEATT
                     SSVYTTTSDDTVTTEEVTYVYDYYYYADYYVEYAEDLMAQYYELYSDLAYQAIAEMQA
                     IEAELNQLNETLVSIDQSLQEISTTLEQGLALAEESIAQLENAAQEAQVKAEAVKGQA
                     QDMIEVIKADQQGRVDEISSIQPHDVPTDQLAVFQSAFDFIDAANLAMGDNKLSHDEL
                     MNLAQLGKNAQAGFMELGKNGGGPGMPGGGTLDFTQLSGRFGDITTQLARGEMPHARK
                     GLGEFELSLGGRPGGGGPGLPGGGGPGGPGAPGGGGPGGPRP"
     gene            complement(5350..6672)
                     /locus_tag="PL000_18950"
     CDS             complement(5350..6672)
                     /locus_tag="PL000_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC91018.1"
                     /translation="METNLFLNSSVDADITPFGFIWGMLRSPLHTLADLARDNGDIAH
                     VKMPKRDFYLLNHPEFIEEVLVKQQANFIKGPSLQRARIILGEGLLTSEGDEHLSQRR
                     ALQPAFHRTKMEEYLPVMGLNTFEHLTRWQHGSVVDMSDEMMRLTLNIALWSFFGSAP
                     EGSVERVSKAMKTLVRLFPLAQLPLPEATRMLFPKFRQAKTDLNIVTETLLANPHSEQ
                     AKRALITILKNEHHDQFTDEQLHAHVLTFLLAGHETTALLLSWCWDMISQDPFVQGKL
                     QAEADAVLGDRFPTSEDIKSLNYTRMVVKETLRMRPPAWALGRQAVQDCTIGGQDIKA
                     GSVVLMSQWVTQHDPRFYDSPLQFRPERWSEIERLPFPRYAFFPFGGGNRVCIGEHFA
                     MTEAIAILAIISRHWQILPTQAKHATPKPSVTLRPNKGVKVLIEKRDV"
     gene            complement(6815..>7528)
                     /locus_tag="PL000_18955"
     CDS             complement(6815..>7528)
                     /locus_tag="PL000_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91019.1"
                     /translation="QNILFTMNAISWLSFQTLEENGEKPMLKIIIADDHQIVRRGLRM
                     TIEAEKDLKVLAEAVNGTEVLALIKKHSPDVVLMDLQMPELDGVETLKAIRPEFPELP
                     VLILTSFTDDAHVYAALRAGASGFLLKEMGGDELVEAIRGAAKGKPQLHPDIAKRLMA
                     HAPMPEDPFGKLTEREREILKLLGRGMSNKEIAVKLTLTEMTVKGYVSDVLMKLGVSD
                     RTQAALMAVRFGLVKPEEL"
BASE COUNT         1647 a         1928 c         2066 g         1887 t
ORIGIN      
        1 gccgaccaca cccgccttgg cggcaggcac ctgcaaataa tacgcgccga agtcaataca
       61 ggtcagcaca cccttcacgg tccctttcgg ggtggcgatg gtggtggcct gactcgcccc
      121 gggcttcaat gcgctcagat cagaggaggc ggcgtaatcg aacacctgac gcacgcgcaa
      181 gcccgtgatc tcaaacacac cgctcgtgga tttatcgtca atgtaataga aatgcggata
      241 tccgctcttc ttcgatttga aatcaatgcc tgcaccttcc gccaactttc taaccttcaa
      301 acgtgcagca ttcaacacat tgaaatcaag tttggcgcgg cgttgtttgc ccttgcgcac
      361 ggtatccacc gagtgcggag cgcatgccag cagcacatcg gcaatgacat cggccagttg
      421 gcgcgatttc ttttcgtcga atccacgctg ggtgatccac ggtgtgccga ggcggattcc
      481 cgaaggggcc gccgcgctct tgtcaccggg gatggtgttg cggttgacaa ccacgccgac
      541 gatatcgagg atacgtgccg cttgatctcc actcaatgtg gttccgtctt cgcctttgat
      601 cgaagagcaa tctacattca gcatgtgaca gttcgtgccg ccgaagggca cgcgcagtcc
      661 gcgcttggtg aattggtctg ccatcgcggc ggcatttttg atggtctgtt cctgcaggcg
      721 tttaaactgt ttggtctgag ccagtttgaa ggtcagggcc agacccgcaa acacgttcac
      781 atgcggcccg ccttgttcgc cggggaagac cgctttgtcg atcttcttgg caacgtcggc
      841 tttggtggtc atcaacactg cgccgcgtgg accatcgagc gatttgtgag tcgtagacat
      901 caccacgtcg gcgatgccga tgggcgaagg aacaactccc gctgcaacca ggccgccgat
      961 gtgggaaata tccgcaagga aatatgcacc gacctcattg gcgatctcgc ggaacttctt
     1021 ccaatcaggg atccatgagt atgaggaata gcccgcgatg agcaatttgg gtttgttttc
     1081 gagcgcgagt tggcgaatgg cttcgtaatc gagtttctcg ttcgcatcca ccgaataatg
     1141 cacagctttg aaccacttgc cgctgcggtt gacgggcgag ccgtgcgaga gatgcccgcc
     1201 atgcagcagg tcgagaccca tcacggtatc gcccacattc aacagcgcct gatagaccgc
     1261 gttgttggcg gggccgcccg aaagcgcctg cacattgacg tagatctggt cggcgctgaa
     1321 tccattggcg gcgaaggttt cagcggcgcg gcggcgggcg agggcttcca cggcatcggc
     1381 atattccacg cccttgtaat agcgtgggtc accgttgcgg cgataatcag caagtcgcgc
     1441 gggataatcg agaatttcat cctcggtcat ccaacgcgac tcttcgtcgg ggtagccttc
     1501 tgcataaata ttttggaatg ccgaagccag cgcttcacgg gtggccatcg gcgcggtcga
     1561 ttcactgggg atcagaatca acttgcggta ttgccgctcc tgttccaatt gggtcaattc
     1621 gtatgtctca gggtcaagtt tttcgagcga accacggaat aaatagtcag ccattctttt
     1681 ctccttttga gagttgattt ttggggattg tcagacagtt gaattgtagg acgggaaggg
     1741 gggaaggtca agtgaaaaaa ggcatattaa ccgatcaagt ggtacggagg agtggttttc
     1801 aaagcgggca tagtgcgaca tactatatga aactaattta caactatagg agatgaaatg
     1861 agcaacatac cccgaatcat gcgtgctgtt ggctggttgc tgtttatggt catgtggatc
     1921 ccctttacct tgatgttcct caacatgccg aacggcaact caaactatga ctctctcatc
     1981 ggactgccga agaccatggc attattcctt gggatcacga tcggtctggc gatcatttcc
     2041 acggctttgc tggtcggttc ctccgtggtc ggctggttga tcggacggat ggtgctggcg
     2101 cggggggaat ctggtacggc ccgtatcatt gccatccaac ccacagggac gcgcgtgaac
     2161 aattactact acggcatgcg ttttacgctg gaggtgcaat cgttcggtga agcttttcaa
     2221 tcacacgcgg agcgcttggt gcccatgcac gatatgacca aataccagat cggcatgacc
     2281 gtcaacgtaa aatatgaccc gatgaccaaa acggttgcaa tggtggattg atagtttgtt
     2341 ttgaagatgg acaggttttg aaaatgggaa acattttcaa aacctgtttt tgattctccc
     2401 tgctttttgc atgggcaagt cggattgcca atccgactta cattgggcgc gcatcgtggc
     2461 aactcttgga aagtgtctga aaagcggact ttcgcagatg ggtttgaaat tcgcggcgaa
     2521 atgttttgtt ggctgttaag aacggcagaa ccattaatac tttctaattt caaacacaca
     2581 atttccatat accccggtca cagcttgatt acatgggcgg aatatcattt gcgaaggttg
     2641 gaggcgctcc aaccacagat caaagataaa gaggtaaaaa atatgaaaaa gaaaatttca
     2701 attctgatcg gcggtttatt ggcggttgcg atgctgttcg gagcggtgag tgtgagcaca
     2761 gcctatgccc agagcggaag tttgactccg ttcagcgatc acggcggccc cggtcacggt
     2821 ggtcgtgggc tgggtcaggc agaactcgaa gcggccgcca aggtactcaa catgaccgcg
     2881 gacgaactgt ccactgcgct gcaggcaggc aagacacttg aagagcttgc caccgccgca
     2941 ggtgtggata ttcaagatgt gaaggatgcc attatggcgg tccatgctga agagatccgc
     3001 gcccgcatcg aagccggtgt ggccgacggc agcatctcgc aggaaaaagc cgactggtta
     3061 ttggaaggtc tggataaagg cttcctggat ggtcccggct ttgggttcgg ccctggcggt
     3121 catcacgatg gtcagcctgc ccaacccgca cagaccacaa ccccataacc attatgaagc
     3181 cataagataa agaaaggagt taaaagaaac agatgaggcg cactcctccg cctcatctgt
     3241 tttgattcgt gaattattcc tctggggcgt aagtgttgaa attgtcgatc tcgaaaatgg
     3301 ctttttcacc gccgttcaat tcaaaaccga gaccaaactt tcctgacatg tattttgtgg
     3361 ttttgacgct tccaacgatg tatccattta cgcagaaagt gtagatgtct ccctgcgcga
     3421 tcacaaccag ttcattgctc tcttctgcat tgacatacct ggaatacccc gtgaacaagg
     3481 gatccagcca gccgccatat tcatgcaccg ccactcgaac actgcccgaa tcacgaatgg
     3541 agaaaaacaa ttgcttttct ccgcgtaccc gaaaaataac gccgtatctt gcatcgcgtg
     3601 gaccagatac tttttggatt tgagcagtga gataaaaatc ccatagattt ttatccagcc
     3661 cggggaattg acgggagtac accccctgca atgcttcaac ctcataatga aggacgccgt
     3721 cggccattcc cagattcgta atatcatagt cgtcggagtt ctttcccagc ggccatagat
     3781 tcaggttttt atcgaaggta ttctccttgg cgataaccca tcccgtcggg atttgcggac
     3841 atttttctgc cgggccgcta aacccaaact gctgcaactc ttcacggaca tcctcatctg
     3901 caaggatgag gggtgatgaa aaacacgcac agatccctgt tgtaaagaga caactcgaga
     3961 tcatcagaaa gataaagacc tttgtttccc aacgcatgga ttctgttgaa ctgttcatgt
     4021 tttccacctc ttgatatgaa attaattata tggtcattgt aaaactttga ttcttgcagg
     4081 tcaagcaaga ggtgacaaat gacatagaaa aaaggatggc agttctgtct gccatccttt
     4141 tttctgttac gggcgcgggc cgccaggacc accgccaccg ggagcaccgg gtcctccggg
     4201 gccgccaccg ccggggagac cagggccacc gccaccgggg cgcccaccga gtgagagttc
     4261 gaattcaccg aggcctttgc gggcgtgggg catttctccg cgggccaatt gagtggtgat
     4321 gtcgccgaag cgtccgctca attgagtgaa atcaagtgtt ccgcctccgg gcatgccagg
     4381 gccgccgcca tttttgccaa gttccatgaa accagcctga gcgttcttgc caagttgagc
     4441 cagattcatc aattcatcgt gggagagttt gttatcgccc attgccaggt tggcggcatc
     4501 aatgaaatcg aaggcgctct gaaagactgc aagttgatcg gtggggacat catgcggctg
     4561 gatgctgctg atctcgtcca cgcggccctg ctggtctgct ttgatcactt cgatcatatc
     4621 ctgcgcctga cccttcacgg cttcggcttt cacctgcgct tcctgggcgg cattttccaa
     4681 ttgggcaatg ctttcttcag ccagtgcaag accttgttcc agcgtggtgc tgatctcctg
     4741 tagtgactgg tcaatggaga cgagcgtttc gttcaattga ttcaactctg cttcgatggc
     4801 ttgcatttcg gcaatggcct gataggcaag gtctgaatac agttcgtaat attgtgccat
     4861 caggtcttcg gcatattcga cgtagtaatc ggcgtagtaa tagtaatcgt acacataagt
     4921 gacttcttca gtggtgacgg tgtcgtcgct ggtggtggtg tacacagaac tggtggtggc
     4981 ttcggttgct gctaccgctt cagcgacggc ctcatcaatg agggctgcaa gttcttcttc
     5041 ggtcagtgcc acataatcca cacttgggcc tggggtgggg gtggcgggca tggcggtgag
     5101 cacattggcg ttgactgtag cctgtgccaa tgcatttgcc tgctgtgtcc ctgcgatggc
     5161 ggtgttgatg acgatgttcg ggtcttgcgc ggctggggtg gcggctgtgc ctccgcaggc
     5221 gagtgtcact gccatgagca aaatgacgct gatgatgatt tgaagtttgt ttttcattgg
     5281 ttcctccaat tgatggttgt ttttcatcgg gttgcttttt gtgtgttgaa tttttttatc
     5341 ttttattcac tatacatctc tcttttcgat aagtaccttt acacctttgt tgggacgcag
     5401 ggttacgctg ggtttgggtg tggcatgttt ggcctgggtg ggaaggatct gccagtggcg
     5461 cgagatgatg gcgaggatgg caatggcttc ggtcatggca aagtgctcac cgatgcagac
     5521 cctgtttccg ccgccgaatg ggaagaaagc gtaacgggga aatggaagcc tttcgatctc
     5581 agaccagcgt tcgggtcgga attgaagcgg cgagtcgtaa aatcgcgggt catgctgggt
     5641 cacccattga ctcatgagca cgaccgatcc agctttgata tcctgtccgc cgatcgtaca
     5701 atcttgcacg gcctgcctgc ccagagccca cgcgggcggg cgcattcgca gagtctcttt
     5761 cacgaccatg cgggtgtagt tgaggctttt gatatcttcc gaggtgggga agcggtcgcc
     5821 gagcacggca tcggcttcgg cctgcagttt gccctgcacg aacggatctt gtgagatcat
     5881 gtcccagcac caggagagca gcaacgcggt ggtttcatgg cctgccagca aaaaggtgag
     5941 tacatgtgca tgcagttgtt cgtcggtgaa ttggtcgtgg tgttcgttct tcaaaatggt
     6001 gatgagcgcg cgcttggctt gttccgagtg cggattggca agcagggttt cggtgacaat
     6061 gttgaggtcg gtcttggcct gcctgaattt ggggaataac atgcgcgtcg cctccggtaa
     6121 cggaagctgg gccagcggaa agaggcggac cagcgtcttc atcgctttgc tcacgcgttc
     6181 caccgagcct tcgggtgcgc tgccgaaaaa cgaccacagg gcaatgttca aagtcagccg
     6241 catcatttca tcagacatat ccacgaccga gccgtgctgc cagcgggtga ggtgctcaaa
     6301 agtgttgagc cccatcacag gcaggtactc ttccattttt gtgcggtgaa aggcaggctg
     6361 taaagcacgg cgctgtgaga gatgttcatc gccttcactg gtgagcaaac cttcgcccag
     6421 aatgatgcgc gcacgctgca ggctgggccc cttgatgaaa ttcgcctgct gcttgaccag
     6481 cacttcttca atgaattcag gatggttgag cagatagaaa tcgcgttttg gcattttcac
     6541 gtgggcaata tcgccattgt cacgtgcaag gtctgccagc gtatgcagcg gactgcgcaa
     6601 catgccccag ataaaaccaa acggtgtaat gtcggcatct acagatgaat tgaggaaaag
     6661 gtttgtttcc atggttcggc tccttgtttg ttgccccaag gatacaggaa gccgtttttt
     6721 atgtatgccc gctgtggagg gtgattcctc cccctatttg gtgggtctat cgcattcccc
     6781 tatttcggtt ttgaaatggg gaaagggatg gtgtttacaa ttcttcaggc tttaccaacc
     6841 caaaccgcac cgccatcaac gcggcttggg tgcggtcgct cacacccagt ttcatcaaaa
     6901 catcgctgac atagcccttc accgtcattt cggtcagtgt caacttaacg gcgatttcct
     6961 tgttgctcat gccgcggccg agcaatttca aaatttcacg ttcgcgctcg gtcaattttc
     7021 cgaacgggtc ttcgggcatc ggcgcatgtg ccatcaggcg tttggcaata tcggggtgca
     7081 gttgcggttt gcctttggcc gccccgcgaa tcgcttcgac caattcatct ccgcccattt
     7141 cctttagtag aaatccgctt gcgcccgccc gcaaggccgc atacacatgg gcatcatcgg
     7201 tgaacgaggt caggatcaac acaggcagtt cggggaattc agggcggatg gctttcaacg
     7261 tttccacgcc atccagctct ggcatttgca aatccatcag caccacatcg gggctgtgtt
     7321 ttttgatgag cgccaaaacc tccgtgccat ttacggcttc ggccagcacc ttcaaatctt
     7381 tttcagcttc gatcgtcatc cgcagcccgc ggcggacgat ctgatggtca tcggcgatga
     7441 ttattttcag catgggtttc tctccgtttt cctcgagcgt ctgaaaactc agccacgaaa
     7501 ttgcattcat ggtgaaaagt atattttg
//
LOCUS       DAOQLC010000162         7430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4060_length_7430_cov_19.978576, whole genome shotgun sequence.
ACCESSION   DAOQLC010000162 DAOQLC010000000
VERSION     DAOQLC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4060_length_7430_cov_19.978576"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..1378
                     /locus_tag="PL000_18960"
     CDS             56..1378
                     /locus_tag="PL000_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC91020.1"
                     /translation="MSTKETLIKKLKEKNAKIAILGLGYVGLPLAVVFGEAGFNVTGV
                     DPDTRKIEALNKGVSYIPDVKTEAVAKLVKDGKFTATADFSVLKEMDAVSICVPTPLR
                     QTGDPDMSFIISATEQLAKYMHKGMVVVLESTTYPGTTRELLLPKLGVAHDLKIGEDW
                     FLAFSPERVDPGREDFTTINTPKVMGGITEACGEVATAWYEGAIKIVHHVSTAEAAEM
                     SKLLENTFRMINIGLVNELAIMCERLGVDVWEVIDAAATKPFGFMKFTPGPGLGGHCI
                     PIDPLYLSWKMKSFNYNARFIELASEINTNMPRYVVARILDAMNDRGMILKGSKVLVL
                     GVAYKPDIDDVRESPALDVIGLLQKKGAVVEYHDPYIPHIHHEYDGWHMDSISDVMKS
                     VQSADAVVVVTNHKSYDYKAIVDSAKFVFDTRNATSKVAKNSENVVRL"
     gene            1387..2367
                     /locus_tag="PL000_18965"
     CDS             1387..2367
                     /locus_tag="PL000_18965"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNC91021.1"
                     /translation="MGHYLVTGAAGFIGARTSELLIGQGHTVVGIDNMNDAYDPRMKE
                     YRLKKLQAMKGFEFVRDDISDKGILKRQAALVSRPFDGVINLAARAGVRFSVDNPWVF
                     LESNVLGTLNMLEICRQYGCKKFILASTSSIYGENPPYPTPETASSSEPMQPYAASKK
                     GAEALAHSYHHLHDIDVTIVRYFTVYGPAGRPDLAMFRFVQWILEGQPIRINGDGNQS
                     RGFTYIDDIARGTIAALKPLGYEVINLGGHEVITINELVEMVEDLTGKKAVVQHGPPN
                     LADMRTNQADVSKAKELLNWHPQVTLREGIGNLINWYNAEREWAKEVLTP"
     gene            2498..3859
                     /locus_tag="PL000_18970"
     CDS             2498..3859
                     /locus_tag="PL000_18970"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNC91022.1"
                     /translation="MRSKFKNDPLMSRVLRSSAHLFTSNTISLGLNVLMTALVFRLLG
                     AAGSGLIALVMGYASTVNSLLSFRMSELVVRYGGEHLEKGEEEKASALIKAAGLTEAA
                     VSVLAFLAVLISSVWASRIFTGETNSALLFIAFAVGLLANFNTETSTGILQVTGKIRY
                     QGTINLIQSVLSLGIVAAAFFLPNQTWVVLLAYLTGKVVLGLGLFFTAQNQLAQKLGA
                     HRAWHRGPLMPFSESRALIRFAVSSNISATIIKVFRESEPLWVGYFLSTEAVGYYKAA
                     YSLVSFLSVPADPLIAATYPEINRLIVQKAWASLRSFLRKVTALAFAVNAAAALGFIF
                     LGQFALILFTGHREFVAAYPTLLALFVGLAFNYTLFWNRPLLLALGLPDFPIWVTLVV
                     GLVKVALAFWLVPQYGILAAGGLLSFYYIASVGVMAVRGVREIGQQENFEPQRHEDAK
                     TQS"
     gene            3906..5033
                     /locus_tag="PL000_18975"
     CDS             3906..5033
                     /locus_tag="PL000_18975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC91023.1"
                     /translation="MRIAVITNSRIPSLTANSIQAMKVIQALMQLDHDVKMFAPKESD
                     PASKQTLLTHYGLRLAPDLELLPSEKHFKRFDFIFHAQRAAKRFKADLIYTWLPQSAA
                     LGLWAGYPVVLEMHADVAGRMGAWWLRQFWKTSGRKLMTVTTSALRHALERSTRLQFK
                     DESLLVAPNGVELERYAELPSPLEARRQLNLPEGLTVGFTGHIYPGRGADLLFELAKQ
                     MPQVNFLWVGGTPELVDFWRGKLTEARMTNVTMTGFVKHEVIPLYQAASDVLLMPYSR
                     SISASSGQDIAEVINPMKMFEYMASGRAIVSADLPSIREVLHEGNAVFVEAGEVGKWK
                     VEIESLLADEPRRNALANQARRDVSQLTWVKREERVMKVIM"
     gene            5055..5285
                     /locus_tag="PL000_18980"
     CDS             5055..5285
                     /locus_tag="PL000_18980"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNC91024.1"
                     /translation="MFTHIAYHAEILNGKPHIVGTRLSIEFILELFASGATREDVIKA
                     YPQLTPEAVEEALRYAAQSVKNEVLLDVRIGT"
     gene            5282..5659
                     /locus_tag="PL000_18985"
     CDS             5282..5659
                     /locus_tag="PL000_18985"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNC91025.1"
                     /translation="MKALDFPLLADENVHPDVVLFLREAGMDVKSVAEQGKYGLPDIQ
                     VLQQATETGRVVLTHDSDFGGLALAGAQFIGIVYVRPGHIHTDFTIKTLQVIRENAPD
                     VTPPFILVAERNGDVVKIRVRQL"
     gene            complement(5730..7322)
                     /locus_tag="PL000_18990"
     CDS             complement(5730..7322)
                     /locus_tag="PL000_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91026.1"
                     /translation="MKPISKIFIIVFLAAILITSCVGPQFMPKTEPTIAMETAMDMVQ
                     TAAVKTQTAQPTLTPTPTPTYLPWQVPTNTPLPRQTYIDPEGWYKVTFPIDWKPVDDK
                     PNHFAGNNGDFFETGYLPEMGYVTNALTACLWFANVEAHEKDNSILNGALLESRRSDF
                     CSVYSKHYDPNVRPHITYEVQENPGADPAHRFVYLKQGFINGINGDAYLLRTKPVNNQ
                     AKFDPGVVPLSPEESDFWGDPASLPSNISVTEFILPPEAQVSPSEIEFYEFVPEELHR
                     EDAQVPTSTPYKKLTTEEQLASLGYELTGRPGELRQLFRDGRILFNNINTDVRGVYNF
                     QTTSGPINAFVVYTAGSTDVYMYGFLVENDSISTWDYNHQDPRFPPILYQDELLWLKT
                     TKNFKHIQVIKSNREVLFSFTAAVEPMYAIHNFSAWNGHWIMDTGNFLIQDGEILNEK
                     FGYPEVFNWGLIKGKPIYFFRKGSRIGISYGDKILPIQYQDVAHGLCCRFSGNNPYIT
                     YNSIRFFGKKDGAWHYVVVKFE"
BASE COUNT         1715 a         1754 c         2035 g         1926 t
ORIGIN      
        1 agcttgtgat atgcaggttt cggcgggttg caaacccgcc ctacgaaagg aaaaaatgtc
       61 taccaaagaa acactcatca agaaattgaa ggaaaagaat gcgaagatcg ccatcctcgg
      121 cctgggttat gtggggctgc cgctggctgt ggtatttggc gaggcgggct ttaacgtcac
      181 cggggtggac cctgacacgc gcaagatcga agcgttgaac aagggtgtgt cttacatccc
      241 tgatgtgaaa acagaagcgg tggcgaaact cgtgaaggat ggaaagttca ctgccacggc
      301 ggatttctcg gtgttgaagg aaatggatgc cgtcagcatt tgtgtgccga ctccgctgcg
      361 ccagaccggt gaccccgata tgtcgttcat catctccgcg accgagcaac tggcgaagta
      421 catgcacaag ggcatggtcg ttgttttgga atcgaccacg tatccgggca cgacccgtga
      481 actgcttctg ccaaaactgg gagttgctca cgacctcaag atcggcgagg actggttcct
      541 ggccttttcg cccgagcgcg tggacccggg ccgcgaagat ttcactacca tcaatactcc
      601 caaggtgatg ggcggcatca ccgaagcctg tggtgaagtg gcaaccgcct ggtatgaagg
      661 tgcgatcaag atcgtgcatc atgtttccac ggcagaggct gcggaaatgt cgaagctgct
      721 cgagaatacc ttccgcatga tcaacattgg cctggtcaat gaacttgcca tcatgtgcga
      781 acgccttggt gtggatgtgt gggaggtgat cgatgccgcc gccaccaaac cgttcggatt
      841 tatgaaattc accccggggc ccggccttgg gggtcactgc attccgatcg acccgctgta
      901 tctttcgtgg aagatgaaat cgttcaacta caatgcacgc ttcattgaac tggcctctga
      961 gatcaacacc aatatgccgc gctatgtcgt ggcacgcatc ctcgatgcga tgaatgaccg
     1021 cggaatgatc ctgaagggct cgaaagtgct tgtgctgggc gtggcttaca aacccgatat
     1081 tgacgatgtc cgtgaatcgc ctgcgttgga tgtgatcggc cttttgcaaa agaaaggtgc
     1141 ggtcgtggaa tatcacgacc cgtacattcc gcacatccac cacgaatatg acggctggca
     1201 catggacagc atcagtgatg tgatgaagtc cgtccaatct gccgatgcgg ttgtggtcgt
     1261 cacgaatcac aaatcctacg attacaaggc cattgtagat tctgcgaagt ttgtgttcga
     1321 tacccgcaac gccaccagca aggttgcgaa gaacagcgaa aacgttgtaa gactttagag
     1381 gaaaccatgg gacattatct ggttacaggt gcggcaggct tcatcggtgc gcggacttct
     1441 gaacttctga tcgggcaggg gcataccgtg gtcggcattg acaacatgaa cgacgcgtac
     1501 gatccgcgca tgaaggaata tcgcttgaag aagctgcagg cgatgaaagg tttcgaattc
     1561 gtccgtgatg acatttcaga caaggggatt ctgaaacggc aggcggcgct tgtcagccgt
     1621 ccgttcgatg gtgtgatcaa tcttgcggcg cgggcgggcg tgcgcttcag cgtggataat
     1681 ccctgggtct ttttggagtc taatgtcttg ggcaccttga acatgctgga gatctgccga
     1741 cagtatggat gcaagaaatt cattctcgct tccacttcca gtatttatgg cgaaaatccg
     1801 ccgtacccga cacctgaaac cgcctcaagc agtgaaccca tgcagccgta tgccgccagc
     1861 aaaaaaggcg cggaggcatt ggcacattcc tatcaccacc tgcatgacat tgacgtgacc
     1921 atcgtccgct atttcacggt ctatggcccc gctggccgcc ccgatctggc catgttccgc
     1981 ttcgtgcagt ggattttgga aggccagccc atccgcatca atggtgatgg aaatcaatca
     2041 cgcggattca cctacattga cgacatcgcc cgcggaacca tcgccgcctt gaagccgctt
     2101 ggttacgaag tcatcaacct tggcgggcat gaggtcatca ccatcaatga gcttgtggaa
     2161 atggtggaag acctgacggg caaaaaagcc gtcgttcagc acggtccgcc caatctggcc
     2221 gacatgcgca ccaatcaagc cgatgtgagc aaggcaaagg aattgctcaa ctggcatccg
     2281 caggtcactc tgcgcgaagg catcggcaac ctgatcaact ggtataacgc cgaacgtgaa
     2341 tgggccaaag aagtcctgac gccgtaaaga atagaatcga ccgaatccaa aacaagtcca
     2401 cggagaaagc agagaatgtc tctgcgccct ccgtggactt tcttgttgtc gcctgcaggt
     2461 gttctctgta gcgggaaatc ttgtgtacaa ttcatccatg cgatcgaaat tcaaaaatga
     2521 cccgctgatg agccgtgtgc tccgttccag cgcacatctt tttaccagca acaccatcag
     2581 ccttggttta aatgtgctga tgacggcgct cgtctttcgt ttgctgggcg cggcgggttc
     2641 gggcttgatc gcgcttgtga tgggttatgc gtccacggtc aacagcctgc tttcgttccg
     2701 catgagtgaa ttggtcgtgc gttatggcgg cgagcatctc gaaaaggggg aagaggaaaa
     2761 agcatccgcg ctcatcaagg ccgcagggct gaccgaggcc gccgtttcgg tgctggcttt
     2821 tttggcggtg ttgatttctt ccgtgtgggc ttcgcgcatt tttacaggcg aaacaaattc
     2881 agcccttttg tttattgcct ttgccgttgg gctgctggca aatttcaaca cggaaacttc
     2941 cacaggtatc ttgcaggtga cggggaaaat tcgatatcaa ggcacgatca atttaattca
     3001 aagcgttctt tcgctgggta tcgttgctgc agctttcttt ttgccgaatc aaacctgggt
     3061 cgttttgctg gcttatctca caggcaaggt cgttttgggg ttggggttgt tctttaccgc
     3121 ccaaaatcaa ctggcgcaaa aactcggcgc gcacagagcg tggcaccgtg gcccgctcat
     3181 gccgttttcc gaatcgcgcg ctttgatccg tttcgcggtc agttccaaca tcagcgcgac
     3241 catcatcaaa gtgtttcgcg agagtgagcc attgtgggtg ggctattttc tttcgacgga
     3301 agcggttggg tattacaagg cggcctattc actggtcagt tttctttccg tgcccgccga
     3361 cccgctgatc gcggccacgt atcccgagat caaccgactc attgtgcaaa aggcatgggc
     3421 atcactgcgg agttttttgc gcaaggtcac ggcgctggcg tttgctgtca atgccgctgc
     3481 cgcgttgggc tttatcttcc tcggtcaatt tgcgctgatc ctttttacgg gtcatcgtga
     3541 atttgtggcg gcatatccaa cactgttggc gttgttcgtc ggccttgctt tcaattacac
     3601 cctcttttgg aatcgtccgc tgttgctggc gttgggcctg cccgattttc cgatctgggt
     3661 cacgcttgtt gtggggttgg tcaaagtggc gctggcgttt tggctcgttc cacaatatgg
     3721 catcctcgcg gcgggcgggt tgctttcgtt ctattacatc gcttcggtgg gggtgatggc
     3781 ggtccgtggg gtgagagaga ttggtcaaca ggaaaatttt gaaccacaaa gacacgaaga
     3841 cgcgaagact caaagttaaa gcttcgtggc tttgtgcctt tgagacttcg tggtaaaact
     3901 ctcacatgcg catcgctgtc atcacaaatt ctcgtattcc atcactcact gccaattcga
     3961 ttcaggcgat gaaggtgatt caggctttaa tgcagttgga tcacgacgtg aagatgttcg
     4021 ccccaaagga atctgatccc gcctcaaagc agactctgct cacgcattac ggtctgcgcc
     4081 tcgcccctga cctggaactg ctcccgtccg aaaaacattt caaacgcttt gactttatct
     4141 tccatgccca acgggctgcg aaacgattca aggccgatct gatctacacc tggctgccgc
     4201 aatctgccgc gctgggattg tgggcaggct accccgttgt cctcgaaatg cacgcggatg
     4261 tggcgggacg catgggcgcg tggtggctgc gtcaattttg gaaaacgagc ggccgcaagc
     4321 tgatgacggt cacaacgtcg gcgctgagac atgccttgga acgctcgacc aggctccagt
     4381 ttaaggatga gtcgctgctg gtcgcgccga atggcgtcga gttggaacga tatgccgaat
     4441 tgccgagtcc gcttgaagca agacgtcaat tgaatctgcc cgaagggttg accgttggat
     4501 tcaccggtca catttacccc ggccgcggcg cagacttgtt atttgagttg gcgaagcaaa
     4561 tgccacaggt gaattttttg tgggtgggcg gcacgcccga gttggttgat ttttggcgcg
     4621 gaaaactgac cgaggcgcgc atgaccaacg tgacgatgac gggctttgtg aagcacgagg
     4681 tcatcccgct ttatcaggcc gcgtcggatg tgctgctgat gccgtattcg cgctccattt
     4741 ccgccagcag cggacaggat attgccgagg tcatcaaccc gatgaagatg ttcgagtaca
     4801 tggcttcggg gcgcgccatc gtttcagcag atctgccttc gattcgagag gtgctgcatg
     4861 agggaaatgc ggtgtttgtg gaggcggggg aggttggaaa gtggaaagtg gaaattgagt
     4921 ctctgctcgc ggatgagcca cgcaggaacg cactggcgaa tcaggcgcgg agggatgtca
     4981 gtcagttgac gtgggtgaag cgggaggagc gggtgatgaa ggttataatg tgagtatcgg
     5041 tcaggagcac aaccatgttt actcatattg cttatcatgc tgaaatctta aacggcaagc
     5101 ctcatattgt tggtacacgc ctgagcattg agtttattct tgaactcttt gccagcggcg
     5161 ccaccaggga ggatgtcatt aaagcatacc ctcaattgac tccagaggcg gttgaggagg
     5221 cattgaggta tgcggcgcag tctgtaaaaa atgaagtgct gttggatgta aggattggga
     5281 cgtgaaagcg cttgatttcc cgttgcttgc ggatgaaaat gttcaccccg atgttgtttt
     5341 gtttctgcgg gaagctggta tggatgttaa atctgttgcc gagcagggga aatatgggct
     5401 tcctgacatt caggttttgc aacaggcgac agagactggc cgtgtcgttt tgacgcatga
     5461 cagcgatttt ggtggtcttg ctttggcggg ggcacaattt ataggaattg tttatgtccg
     5521 tccgggtcat attcatacgg atttcacgat aaagacttta caagtcatac gagaaaatgc
     5581 tccagatgtg acaccgccat ttattctcgt tgctgagcgg aatggggatg tcgtgaagat
     5641 cagggtcaga caattgtagc aattgtgcaa tgaaaagatg ataaaaacag caactgcttg
     5701 aaggcggttg ctgttttttg ttactgtagt tattcgaatt ttacaaccac ataatgccac
     5761 gcaccgtctt ttttgccaaa gaagcggatg gagttataag ttatgtatgg gttgttgcct
     5821 gaaaacctgc agcataaccc atgtgccaca tcctgatatt gtatgggcag gattttatcg
     5881 ccataggaaa tcccaatcct tgaacctttg cggaagaaat agattggctt gcctttgatc
     5941 aagccccaat taaacacttc tggataacca aatttctcgt tcaggatttc accatcttgg
     6001 attaagaagt tgccggtgtc cataatccaa tgcccgttcc aggcgctgaa attgtgtata
     6061 gcgtacattg gctcaactgc tgctgtaaaa gaaaataaaa cttctcggtt gctttttatt
     6121 acttgtatat gcttaaagtt tttagtggtt ttcagccaaa gcaattcgtc ttggtataaa
     6181 atgggaggaa agcgggggtc ttgatggttg taatcccaag tgctgatcga atcattttca
     6241 acaaggaaac catacatgta gacatctgtt gacccggcag tgtagacaac aaaagcattg
     6301 attggacctg aggtagtttg aaagttgtaa actcctcgta catctgtgtt tatgttattg
     6361 aacaaaatcc tgccatcgcg gaacaattgt cgtaattctc ctggacgacc tgttaattca
     6421 tacccaagag acgcaagttg ttcttcagtg gtaagttttt tgtagggagt tgatgttgga
     6481 acttgtgcat cttcacgatg taactcctct ggaacaaact cgtagaactc gatttcggaa
     6541 ggacttacct gcgcttctgg aggcaaaatg aattcagtaa cggagatgtt ggacggcagg
     6601 gacgcagggt ctccccaaaa gtctgattct tcagggctta ggggaacaac tccggggtcg
     6661 aattttgcct ggttgttaac tggttttgtc cgcaataagt atgcatcccc attaatgccg
     6721 ttaatgaaac cttgttttaa gtaaacaaag cggtgcgcgg ggtcggctcc gggattttcc
     6781 tgtacttcat aagtgatatg tggacggaca tttgggtcat aatgttttga atatacacta
     6841 caaaagtctg atctccgcga ctctagaagt gctccattga gtattgagtt gtctttttca
     6901 tgagcctcaa catttgcaaa ccagagacag gcggttagcg catttgttac ataccccatt
     6961 tctggcaaat accccgtttc aaaaaagtca ccattatttc ctgcaaagtg gtttggtttg
     7021 tcatcaacgg gcttccaatc aataggaaaa gtgactttat accaaccctc ggggtcaata
     7081 taagtttgtc ggggtagtgg ggtgtttgtg ggaacttgcc agggaagata cgttggcgtt
     7141 ggtgttggcg taagcgtggg ttgcgctgtt tgagttttga cagccgcagt ctgtaccatg
     7201 tccattgcgg tttccatggc aatggtgggt tcggtctttg gcataaattg tggcccaaca
     7261 caagatgtga taagtattgc tgccagaaaa acgatgatga atattttgct gataggtttc
     7321 aagattttcc ttccctttgt tcagttcttg tccaacttta ttttaccaag tggtgcccct
     7381 caccactgct gacaaatcca tcatcaatcg cgttcttttt agcgaaagtg
//
LOCUS       DAOQLC010000163         7275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4211_length_7275_cov_23.932825, whole genome shotgun sequence.
ACCESSION   DAOQLC010000163 DAOQLC010000000
VERSION     DAOQLC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4211_length_7275_cov_23.932825"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..954)
                     /locus_tag="PL000_18995"
     CDS             complement(70..954)
                     /locus_tag="PL000_18995"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNC91027.1"
                     /translation="MKKYWPTTRDYLLIIASSLIQAVSLRLFFIPANLASGGVSGISQ
                     LINHYTGWPIGLMVLIGNIPLFLLGWRFLGGVKFAMRTAVAILTYSLFTDLILKVPYF
                     VEYSQILISDLSGDIFLNSLYGAIISGIGYGMVYRARGTSGGSDILARILNNWRGIPM
                     TQSYLIVDTAVILSAGFVFGWKEALYAMITLYVSGIVSETVLEGGGTVRTAMIVTAKP
                     EEVSNRVIEELERGVTWLEGRGAYTGNSRPVLYCVVNRAEIASLKAIVHESDPEAFMV
                     IGVAHEALGEGFKPLKGR"
     gene            complement(991..3333)
                     /locus_tag="PL000_19000"
     CDS             complement(991..3333)
                     /locus_tag="PL000_19000"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNC91028.1"
                     /translation="MKLPALRMQDLTFGLSLLALICLPIALSEIVRDAGVSLLLPLTL
                     IGAVLAWALAGWGVRKLFSGFILLLLGPLVLYVRIGQLWDSLFEWVRQFFALLPNFFD
                     AAFNDTQLDSTLLAAAQDVFTQRALAFSGRITLWVTAMVRGVQIEDPVVRTLIWCLAI
                     WLIAVWAGWQIYRNQKFTLGMFPSTVVLAFVLDYTGKEKEILWFHLALLLFLYGLTNY
                     QSLQKRWDARKIDYSESTSIDTLAATGALTVVLVAVAFLVSTFSIKDILDDFRERRAG
                     SNESQAESLGLESVKDNFRVTGFGNGLPRSYLLKAGPEISTQLAMTISTGELPPMSRN
                     AHPNVPRYYWRTLTYSIYTGAGWSNPTIGAEDIPANQPLIETENANQRIVRAQITYPD
                     DFSERLYWAGTFISTDVPIQAAWVHRSQDANPLDTDLLAAFAPVETYTVESAILNVSV
                     QELRDSPSVYPDWVRKQFLGLPDSVPERVLSLARDLTASEANAYDRAIAIQNYLREFP
                     YTLDISSPPAGRDVTDYFLFDLKQGYCDYYATSMAVLARAAGLPARLVVGYANGNYDL
                     ERARYIVTENYAHSWVEIYFAGIGWVEFEPTASEPVILREDQNDAAASTADSQPEEDQ
                     AQNKFTLPLQNIWASIFALLALASIGIGIDTLRLKRIPPAQTIQILYKRLRRLARPVT
                     GYASKNRTAYLYSSDLIRSLSALDASPRLQNWLAPARNEIELLTELFSRSLFAPTEPS
                     RAEAEQAIQAWASLQWRLALANLLIVVARHNMSRYQPPRL"
     gene            complement(3330..4604)
                     /locus_tag="PL000_19005"
     CDS             complement(3330..4604)
                     /locus_tag="PL000_19005"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC91029.1"
                     /translation="MKLNSVLPPLLSLTMLVMQILDPSKIWQALFVAFSGLWLAAWWW
                     ARSLKLNVRLTREVRFTWAQVGDALEERFTLTNHGHVPATWVEVVDHSTLPDYSAACA
                     VGVDQASVNVWSTRAVCKRRGVYELGGTTLRCGDPFGIYSVEIFLPEKTTLVVMPPVI
                     PLPTVEITPGGWMGDGRPRPNLVDQTVNAATVREFTQGENPKIIHWPTTARQRKFYAR
                     QLDGAPASDWWIALDADAGVQAGADWENTLELGIILAASLADRSLRARHSVGLLASGR
                     QTTWLKPQSGERHRLEIFRALAMLEPGQLPLAQLLERANSSLGNRISLIVITPSIKTD
                     WIPALTHLMWKGIHPTVLLMDPSSFGAPQKADALAGIFAQLGIPRFILNRSLLQQPEA
                     HPGEGQWAWRITPSGKAISARPPSDMTWKRLG"
     gene            4685..5860
                     /locus_tag="PL000_19010"
     CDS             4685..5860
                     /locus_tag="PL000_19010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNC91030.1"
                     /translation="MSITLNPNREKSLLRRHPWIFASAIRHVDETHASGSTVDLLSSE
                     GQFLARASYSPNSQIRARVWTFNDEPVDEEFFRKRIRAAIEMRSVLSGSSKSLRLIHA
                     ESDGLPGLIVDRYEDVLVLQSLTAGSEFWKETIADLLVEETGIATIYERSDADVRELE
                     GLKPITGILRGTITNLQLPITEYGLEFKVDIAHGHKTGFYLDQRENRRRVGELSKNRD
                     VLNCFCYTGGFSIHALANGAKSVLSVDSSADALALLEENIALNQLPADRHTSLEGDVF
                     QLLRKFRDANRSFDLIVLDPPKFAPTAAHAEKASRAYKDINLLAFKLLRRGGLLFTYS
                     CSGGIDAALFQKIVASAALDAGVDATIIEHLSQGSDHPVSLHFPEGMYLKGLVCVKG"
     gene            complement(5887..7011)
                     /locus_tag="PL000_19015"
     CDS             complement(5887..7011)
                     /locus_tag="PL000_19015"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HNC91031.1"
                     /translation="MSGLNNAIQLIREGRKEEARKILELILRSEPGNIQAWFWSVETY
                     SSLEKRIQVLELCLEINPGNAQVLQALQKLRSQRPAQNAVTPPPAQPTHQTYSAPLRD
                     EPPKPAASSYEPMYYEDKPKASSSTHPYFDDEPSAETDHYAYTSTPQKKDTISYSDTF
                     SLTKPKPTRKSYAFHEVWMIALSMLRMDAYEDTLNDPEATTGRALEWIAYAGIISGLL
                     FPFTLGASPQFAELQTMSELNQFFERFGTVAFTLILALAMAVISPLLSVISLTISAWF
                     QNLIAAFMGGHGNFTRTIYAQAAYLAPLSILIAVIGIIPVVGQCFNSLVGIYGLVLNI
                     RALQAAHSLTTGKAIGVIFLPSIFMIGFLCLAFFIVSAFN"
BASE COUNT         1677 a         1903 c         2065 g         1630 t
ORIGIN      
        1 atgcgaagaa gaagtaacga acatctttcc tctttcttct ttatcaatgg aagaaagagg
       61 aaagaaaaat tatctgcctt tgagcggttt gaacccttcg cccagggcct catgtgcgac
      121 cccgatgacc ataaatgctt cggggtcgct ttcgtgtacg atggctttta gcgaagcgat
      181 ttcagcacgg ttgaccacgc aatacaaaac tggacgtgaa ttgcccgtgt aggcgccgcg
      241 gccttcgagc caggtcacgc cgcgttcgag ttcctcgatc acacggttgg aaacctcttc
      301 gggtttggca gtgacgatca ttgccgtgcg gaccgtgcct ccgccttcga gcaccgtttc
      361 tgagacgatg ccgctgacgt acagcgtgat cattgcgtac aacgcttcct tccacccgaa
      421 gacaaagccc gcagaaagga tcactgccgt atccacgatg agatagctct gggtcatggg
      481 gatgccgcgc cagttgttga ggatgcgcgc aaggatatcc gagccgccgc tggttccgcg
      541 tgcgcgatag accatgccat agccgatgcc gctgatgatg gcgccataca atgagttgag
      601 aaagatatcg ccgctcaagt cgctgatgag gatctgcgaa tattcgacaa aataggggac
      661 tttcaaaatc aggtcggtga acagcgagta ggtgaggatc gccaccgccg tgcgcatggc
      721 gaacttgacc ccgccaagga aacgccagcc cagtaagaac agcggaatat tgccgatcaa
      781 caccatcaaa ccgatgggcc agcctgtgta gtgattgatg agttgcgaaa tgccgctcac
      841 gccgcccgag gccaggttgg cagggataaa gaacaggcgc aaactcaccg cctgtatcag
      901 gctggatgcg atgatcaaaa gataatcccg cgtggtgggc cagtattttt tcatgaaact
      961 tcctttttta aaactaaatt tgtggaggca ttatagccga gggggttggt aacgcgacat
     1021 attatgtcgc gctaccacaa ttaataaatt tgccaatgcc aatctccact gcaaactagc
     1081 ccaggcttga atggcttgct ctgcttcggc gcgggatggc tccgtcggcg cgaacaggct
     1141 gcgcgagaac aattcggtta atagttcgat ctcgttgcgt gcgggtgcaa gccagttttg
     1201 caggcggggt gaagcatcaa gtgccgataa acttcggatg agatcagagg aatacaaata
     1261 cgctgttctg tttttcgagg cgtaccctgt cacaggtcgg gcgaggcggc gcaggcgttt
     1321 gtacaagatc tggatcgtct gtgcgggcgg aatccgcttg aggcgcaggg tgtcaattcc
     1381 gataccaatg ctggcaagtg caagcaaggc aaagatggaa gcccaaatgt tttgcagcgg
     1441 cagtgtgaat ttattctgcg cctgatcttc ttcaggttgt gaatcggctg tagaggcggc
     1501 tgcatcgttt tggtcttcgc gcaaaatgac gggttcgctg gcggttggtt cgaattccac
     1561 ccagccgatg cccgcgaaat agatttccac ccaggagtgg gcgtaatttt cagtgacgat
     1621 gtatcgtgcg cgttcgaggt cgtagttgcc gttggcatag cccaccacca gccgtgcggg
     1681 cagccctgcg gcacgcgcca gcaccgccat ggaagtagcg taataatcgc aatacccttg
     1741 ttttagatcg aagagaaaat aatcggtcac atcgcggcct gcgggcggcg acgaaatatc
     1801 gagcgtgtac ggaaattcgc gcagataatt ctggatggcg atggcgcggt cgtaggcatt
     1861 ggcttcggat gcggtgagat cgcgcgccag agataacacc cgctcaggca ccgaatcagg
     1921 cagaccgaga aactgcttcc gcacccaatc aggatacacg ctgggagaat cgcgcaattc
     1981 ctgcacgctg acgttgagga tcgccgattc aacggtgtag gtttccacgg gtgcaaatgc
     2041 cgcgaggagg tctgtatcaa gcgggttggc atcctgcgaa cgatgcaccc atgcggcttg
     2101 gatcggcaca tcggtgctga tgaatgtccc cgcccaatac aggcgttcgc tgaaatcatc
     2161 ggggtaggtg atttgggcgc ggacgatgcg ttggtttgcg ttttcagttt cgatcagcgg
     2221 ctggttcgcc gggatgtctt ctgcgccgat cgttgggttc gaccaccccg cgcccgtgta
     2281 aatggaatag gtcaaggtgc gccaataata gcgcggcaca tttgggtgtg cattgcgcga
     2341 catgggcggc aattcgcctg tggaaatggt catggctagt tgggtggaga tctctggccc
     2401 tgccttcaac aggtaggaac gcggcaggcc gttgccgaat cctgtgaccc tgaaattatc
     2461 tttgaccgat tccaacccga gcgattcagc ttgcgattcg ttcgagcctg cacgcctttc
     2521 acgaaaatca tcgaggatgt ctttgatcga aaacgtggaa acgaggaagg caaccgccac
     2581 caatacaacg gtcagcgcgc ctgtggcggc gagggtgtca atgctggtgc tttcggaata
     2641 atcaattttg cgggcatccc aacgcttttg cagggattga taattggtca gcccgtacag
     2701 aaataaaagc agtgcaaggt gaaaccagag aatctccttt tccttgcctg tgtaatcgag
     2761 gacgaatgcc agcaccacag tggatgggaa catgcccagc gtaaattttt gattgcggta
     2821 gatctgccag cctgcccaca ctgcgatcaa ccagatggcg aggcaccaga tcaaggtgcg
     2881 cacaacgggg tcttcgatct gcacgccgcg caccatggcc gtgacccaca atgtgatgcg
     2941 tccgctaaat gccagcgccc tttgcgtaaa aacatcctgt gcggcggcca gcagcgtgga
     3001 atcgagttgg gtgtcgttga aggctgcatc gaaaaagttg gggagcaggg cgaagaactg
     3061 cctgacccat tcaaacaatg aatcccacaa ctgaccgatg cggacgtata aaaccagcgg
     3121 acccagcaaa agcaggatga accctgaaaa taattttcgc acgccccagc cagccagtgc
     3181 ccatgccagc accgcgccga tcaaggtgag cggcagcaac aaactcacac ccgcatcgcg
     3241 gacgatctcg cttaatgcga tcggcaggca gatgagcgcg agcagtgaaa gcccgaaggt
     3301 caagtcttgc attcgcaagg cgggcagttt catccgagcc tcttccaggt catgtcgctg
     3361 ggtgggcggg cggagatggc tttgcccgag ggtgtgatgc gccaagccca ctgcccttcg
     3421 ccaggatgtg cttcgggttg ttgcagcagg ctgcggttaa ggatgaagcg tggaattccc
     3481 aattgggcga aaatgcctgc aagggcgtcc gctttttgag gggcgccgaa tgacgagggg
     3541 tccattaaaa gcacggttgg gtgaatgccc ttccacatta aatgggtgag tgcgggaatc
     3601 caatccgttt ttatggaagg ggtgatgaca atgaggctga tgcgattgcc gagcgatgag
     3661 ttggcgcgtt cgagcaattg cgccagcggc agttgaccgg gctcaagcat ggccagcgcg
     3721 cggaatattt ccagccgatg ccgttcaccc gattgcggtt tcagccaggt ggtttgcctg
     3781 ccgctcgcaa gcaggcccac cgagtgccgc gcgcgcagac tgcggtcagc cagagatgcg
     3841 gccaggatga tgccgagttc aagcgtgttc tcccaatcgg cgcctgcctg cacacctgca
     3901 tcggcatcga gtgcgatcca ccaatcgctg gcgggtgcgc catccaattg gcgcgcataa
     3961 aattttcttt gccgcgccgt ggtgggccag tggatgattt tcgggttttc gccctgagtg
     4021 aattcgcgca cggtggccgc atttacggtt tggtcaacaa ggtttgggcg tgggcgtcca
     4081 tcgcccatcc aaccgcctgg tgtgatctcc actgtgggca ggggaatcac aggcggcatg
     4141 accaccagcg ttgttttttc aggcagaaaa atttcaaccg aataaattcc gaaagggtcg
     4201 ccgcagcgca gggttgtgcc gcccaattca tacacaccgc ggcgtttgca aacagcccgc
     4261 gtgctccaca cattgacgga tgcctgatcc acgccaaccg cacaagccgc cgaataatcg
     4321 ggcagggttg aatggtccac aacttcaacc caggtggcgg gcacatggcc gtgattggtc
     4381 agtgtaaaac gctcttcgag tgcatcgccc acctgcgccc atgtgaatcg aacttcgcgc
     4441 gtcaggcgca cgttcagttt caaagaccgc gcccaccacc atgccgccag ccacaatccg
     4501 ctgaacgcca cgaacaacgc ctgccagatc ttcgatgggt cgaggatctg catcaccagc
     4561 atggtgagcg ataacagcgg cggcaggacg gaatttaatt tcatgcgcgc gatcatcttc
     4621 ccattataag ctcagctttt tgcaggggag gcggatcgaa aagattttgc ggtaagataa
     4681 gcgcatgtcc atcactctca acccaaaccg cgaaaaaagt ttgctgcgcc gccatccgtg
     4741 gatctttgcc agcgccatcc gtcatgtgga tgaaacccac gcatcaggct caaccgttga
     4801 cctgctctcg tccgaggggc agttccttgc ccgggcatcc tactccccaa attcgcagat
     4861 ccgcgcccgt gtgtggacat ttaacgatga acccgtggat gaagagtttt tccgcaaaag
     4921 aatccgcgcg gccattgaaa tgcggtctgt gctgtcgggc tcatcaaaat ccctgcggct
     4981 gatccacgcc gagtcagatg gcctccccgg gctgatcgtt gaccgatatg aggacgtcct
     5041 tgttctgcaa tcgctcaccg ctggctccga attctggaag gaaaccatcg cagacctgct
     5101 ggtggaagag acgggcattg ccacgatcta cgaacgctcc gatgccgacg tgcgcgaact
     5161 cgaaggcttg aaaccgatca caggtattct gcgcggcaca attaccaatc tccaattacc
     5221 aatcaccgaa tacggcctcg aattcaaggt tgacatcgcg catggccaca aaacgggttt
     5281 ctacctcgat cagcgcgaaa accgccgccg tgtgggtgaa ttgtcgaaaa accgcgatgt
     5341 attaaattgc ttctgctaca caggcggatt ctccatccac gctttggcga acggggcgaa
     5401 atcggttctg tccgtcgatt cctctgcgga tgcgctggcc ctgctcgaag aaaatattgc
     5461 cttgaatcaa ctccccgctg accgccacac ttctcttgaa ggcgatgttt tccaactgct
     5521 cagaaaattc cgcgatgcga atcgatcttt tgatctgatc gtgctcgacc cgcccaaatt
     5581 cgcacccacc gccgctcatg ccgaaaaagc ctcgcgggct tataaagaca tcaatttgct
     5641 ggccttcaag ttgctccgcc gcggcggatt gcttttcacc tattcctgct ccgggggcat
     5701 cgatgcggcc ctgtttcaaa agatcgtcgc ctccgccgcc ctcgatgcag gcgtggatgc
     5761 gacgatcatt gagcatttgt cacagggcag cgatcacccg gtcagtttgc atttccccga
     5821 agggatgtat ttgaagggac tggtttgtgt gaaggggtga tttccacttg cagggtttac
     5881 cacctgtcag ttaaaggcac tgacgatgaa aaacgccagg cacaggaaac ctatcataaa
     5941 aatgctgggg agaaagataa ccccgatcgc ttttccggtg gtcaaagaat gagcggcctg
     6001 caaggcgcgg atatttaaca caagtccgta gatgccaacc agactgttga agcattggcc
     6061 gacaacgggg atgatgccaa tgacggcgat caaaatggat aaaggtgcaa gatatgccgc
     6121 ttgtgcatag atcgtgcggg tgaaatttcc atgtccgccc atgaaagcgg cgatcaagtt
     6181 ctggaaccat gcgctgatcg tcaggctgat cacactcagc agcggggata tcacagccat
     6241 tgccagtgcc aggatcaatg taaaggcgac cgttccaaac ctttcgaaga actgattgag
     6301 ctcagacatt gtttgcaatt cggcgaattg tgggctggcc ccgagcgtaa atgggaataa
     6361 caatccgctg atgatgcctg cataggctat ccattcaagg gcgcggcctg ttgttgcttc
     6421 agggtcattg agcgtgtctt cataggcatc catccgcagc atggagagtg cgatcatcca
     6481 cacttcgtga aacgcatagg actttctcgt tggcttgggt ttcgtgagtg aaaaagtatc
     6541 ggagtaactg atggtatctt ttttctgcgg tgtggatgtg taagcgtaat gatctgtttc
     6601 agcggatggt tcgtcgtcga aatagggatg tgtggatgaa gaagcctttg gtttatcttc
     6661 ataatacatg ggctcataag acgaggcggc aggttttgga ggctcgtctc tgaggggtgc
     6721 ggaataggtt tggtgcgtcg gttgtgcagg cggcggagtc actgcatttt gggctggccg
     6781 ctggctgcgt agtttttgca aagcctgcaa gacctgtgca ttgccggggt tgatctcaag
     6841 gcagagttcc agcacctgga tccgtttttc aagggaggaa taggtctcca cagaccaaaa
     6901 ccacgcctgg atattccccg gctccgaccg taagatcaat tcgagaatct ttcgcgcttc
     6961 ttccttccgc ccttcgcgaa tcaactggat ggcattgttt agacctgaca taggacctca
     7021 tggaaaatgc agaatacggg gttatgtggt caacgaataa aaatgaacga tttgtagcgt
     7081 gaatgcctgt aatgtaacac tgcctagtat aaaacctttt ttatgttttc aagttgatct
     7141 accagcagac tcgatgattt ttgcaggtct attcttcaat gagttgctca tcgggtggta
     7201 aggattgctt gaatgtgaaa gcgaacggcg ttgggccatg cgactccaaa tgagccagcc
     7261 gcttctttgc ctcat
//
LOCUS       DAOQLC010000164         7148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4340_length_7148_cov_27.118286, whole genome shotgun sequence.
ACCESSION   DAOQLC010000164 DAOQLC010000000
VERSION     DAOQLC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4340_length_7148_cov_27.118286"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="PL000_19020"
     CDS             <1..1164
                     /locus_tag="PL000_19020"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC91032.1"
                     /translation="SEKDELGNAFSRMITGLRDAVGQVAESASAVSSAASQLASASEQ
                     SGDAARQIATTIQQVAMGITQQTTGVTKTSASVEQMNRAIDGVARGAQEQATAISKAS
                     QVTSRISTAIEQVANNAQSVTRDSAEAAAHSRNGAVTVQETIAGMEAIRSKVGLSATK
                     VEEMGTRSEEIGAIVETIEDIASQTNLLALNAAIEAARAGEQGKGFAVVADEVRKLAE
                     RSSLATKEIAALIKGIQKTVNEAVGAMKASASEVEAGVERANSAGVVLEKILVAAESV
                     YKQAEEAGNAASKVSVAAAELVESVDAVSAVIEENTAATEEMAANSSELTQSIENIAS
                     VSEENSASVEEVSASTEEVSAQVEEVSASATSLMDMAKQLSQVVARFKLAAEK"
     gene            1317..1769
                     /locus_tag="PL000_19025"
     CDS             1317..1769
                     /locus_tag="PL000_19025"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC91033.1"
                     /translation="MEQQLVVFELANEFYGINIAMVESIIKMQAITQLPQTPVYVKGV
                     TNLRGSVLPVIDLRTRFALDAKEDTKQTRIMIVTMGSIKAGIIVDGVSEVLRVADDSV
                     EPLPPMINSVDSAFLKGIVRLEDRLIIMLELSKVLNLDEQKSLEALAA"
     gene            1872..4130
                     /locus_tag="PL000_19030"
     CDS             1872..4130
                     /locus_tag="PL000_19030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC91034.1"
                     /translation="MDRILGRFSIGFKLGGSFFFIIVLIAVTLSLSYSDLNQLNLGMA
                     SMYHDHTIPIQNLSEARALVGRIKSNIQLNLQIPKPEAEDEKTNGAPSCDSCHVFETS
                     GDHHVKDGESAGDTNRCQVCHSAQFEDSEHGLSTASIMSEQSCSSCHPSEVIDKQHHQ
                     VEQDIAADVARVNEIIAEYRQSGLLTVEEEAELARFDKAWADYQVIITEIISQMDSGE
                     AQTALHRVVGGDALKSQQAVDESIGKLVAVIQELAHQSQEQGANTFNTAAREMLSVGL
                     FAILLSIGLGFVITANIRTPVEVMAKGLQNMRQGDLDWNVSDAVKESIVQRSDELGVA
                     GMGFDGTLQYMREMAGVAGQIASGDLTVLVNPRSEKDEFGLAFSKMVDSLNALIRLVM
                     ESTENLNSASRQLALSSGQSGEATRQIATTIQQVAMGITQQTAGITKTSSSVEQMNRA
                     IDGVARGAQAQAEAIGKASLVAGRIRAAIKQVTSNAQTVTHDSAEAANHSRNGANTVR
                     ETIAGMEVIRSKVNLSAAKVEEMGARSGEIGVILETIEDIASQTNLLALNAAIEAARA
                     GEQGKGFAVVADEVRKLAERSSLATKEIAKLIKGIQGTVNEAVEAMNASASEVEAGVV
                     RANSAGVVLENILVAAESVYKQAEEAGTAAEKVEVASGELVEAVDAVSAVVEDNTAAT
                     EQMAGNSSELTQSIENMASISEESSAAVEEVSASTEEVSVQVGEVSSSADSLMKMAQQ
                     LSQVVSRFNLKG"
     gene            4136..6457
                     /locus_tag="PL000_19035"
     CDS             4136..6457
                     /locus_tag="PL000_19035"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC91035.1"
                     /translation="MKNVVHIPLHRSFLGRLLFFMLLIGILPVVINAAISYNLAQKAL
                     DEATDEMQTIIEKDQTAYILAWANERNQDIVTLAGVARITSMNPETANEAIKQYYRLW
                     GGYETIFLVDMEGKSIATSDDHPLDVAERTYFQEALKGRVVMSEPLTSKATGNLIIVF
                     AAPVRASGGLIVGVVGETVPVSAITQLLIKNRTGETSESYLVNSEGYFVTTPRFVKEM
                     QKAGLIEGQPELKYQLQTTASKELQAARSGQGIYENYLGEEVIGRYTWLPDLKLGLIS
                     EKQTSEAKAASDRLATFSLITIVLSVVLIFVVAFTIARGITRPVQLMANTANRLALGD
                     IDQNLDYASRDEYGILADSIRLMLDYQKEMATVANSISSGNLTESIQPKSEKDQLGQA
                     FKQMIESLREAIQRVAFSANTLTFASEQLAISADQAGRATSQIATTVQQVAKGITMET
                     ESVTRTAFSVEQMSQTIGTVAQGSQDQTKSVTSATHITNQINEAIQQVAGNVQAVTNV
                     SAGAAEAARMGAQTVVGTVKDMQLIKSKVNLSAQRVADMGAQSDQINVILETIEDIAS
                     QTNLLALNAAIEAARAGEHGKGFAVVADEVRKLAERAGRATREIGGLIKNIQHTVSEA
                     VIAMDEGGQEVESGVQMANQAGKALEDILKAAEEVYRQAEQADHGAELMRSASSSLVS
                     SVDEVSTVVEGNMAAMQSMTAGMAEMTRTIENIAAVSEENSAAIEEVSASAEEMSAQV
                     EEVTASAQSLMEMAKSLQEIVQQFNLGESVASA"
     gene            complement(6540..>7148)
                     /locus_tag="PL000_19040"
     CDS             complement(6540..>7148)
                     /locus_tag="PL000_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC91036.1"
                     /translation="EMNDEAGISSTLINLAVALFNQGRYEEEKPLLQESIEISRRINQ
                     PRNLAGALGNLGTILLKEKNYAGALELFLQGLETHRNTGYRYGIAIALDNVGTAYFHL
                     ENDSEALYYIKQSIREAQDIKADFIALDALVWVGGIYARRGELEKAFEMWEMIRSHNK
                     TDPETIQNLDELMSKFSIGTPLEEQGRMRSFTEIVEEIMQTK"
BASE COUNT         1772 a         1621 c         2053 g         1702 t
ORIGIN      
        1 tctgaaaagg atgaacttgg aaacgccttt tccagaatga tcaccgggct gcgcgatgcg
       61 gtgggacagg tggcagagag tgccagtgcg gtctccagcg cggcatccca actggcatct
      121 gcttctgaac aatcgggaga tgccgcccga cagatcgcca caaccattca acaagtggca
      181 atgggaatta cccaacagac cacgggcgtt accaagacct ccgcttcagt ggagcagatg
      241 aaccgtgcca ttgatggggt tgccagagga gcacaggaac aagcaacggc catcagcaag
      301 gcttcacagg tcacttcacg gatcagcacc gcgatcgaac aggtggcaaa caatgcccaa
      361 tccgtgacac gagattcggc tgaagctgcc gcccattccc ggaacggcgc tgtaaccgtt
      421 caggagacca tcgcaggcat ggaggcgatc cgcagcaaag tggggctctc tgcaacaaaa
      481 gtggaagaga tgggaacacg ctctgaagag atcggcgcca ttgtggaaac gatcgaagat
      541 attgccagtc agaccaattt gttggctttg aatgctgcca ttgaagcagc ccgcgctggt
      601 gaacaaggca agggttttgc agtggtggct gatgaagtgc gcaaactggc cgaacgttcc
      661 agcctggcta caaaagaaat cgctgccctg atcaaaggta ttcagaagac ggtcaatgaa
      721 gctgtgggag ccatgaaggc ttctgccagt gaagtggaag cgggggttga gcgggccaac
      781 tcggctggtg tagtgttgga gaagatcctg gtggcggctg agtctgtgta caagcaggct
      841 gaagaggctg gcaatgcagc ttccaaggtc agtgtcgctg ctgcagaatt ggtcgaatca
      901 gtggatgccg tttcagcggt cattgaagag aacacagctg ccactgaaga aatggccgcc
      961 aattcctccg aattgacaca atccattgag aatatcgcca gtgtcagcga agagaacagt
     1021 gcttcagttg aagaagtttc cgcctccact gaggaggtct cggcccaggt ggaggaagta
     1081 tctgcttcgg caacctccct catggatatg gccaaacaac tttcgcaggt ggttgcccgt
     1141 ttcaaactgg cagcggagaa ataagcgatc tttatggaga actttctcat tcacttgaga
     1201 aagttctcca tcacgggttt cttgcattag caccgtcatt gccttgaatg aaataaagaa
     1261 caagcctttt gcaagatgcc cactgtataa gcagactatt tttgaaagca ggtaccatgg
     1321 aacaacaact ggtggttttt gaactggcaa atgaatttta tggcatcaat atcgccatgg
     1381 ttgaaagcat catcaagatg caggcgatca cccaacttcc gcagacccct gtgtatgtga
     1441 agggtgtgac caatctgcgc ggcagtgttc tgcctgtgat cgatctgcgc acccgttttg
     1501 cattggatgc aaaggaagat accaaacaga cccgcatcat gatcgtcacc atgggaagca
     1561 ttaaggcggg cattatcgtg gatggggttt cagaagtgtt gcgtgttgca gacgattctg
     1621 tagaacccct gccgccgatg atcaattcgg tcgattctgc tttcttgaag ggcattgtcc
     1681 gcctggaaga tcgcctgatc atcatgctgg aattgagcaa ggttttgaat ctggacgagc
     1741 agaagtcgtt ggaggcttta gcggcttaga ttctgtcatt cggactgagg caggcttggg
     1801 cggggcggaa gcgcggtccg tcctcctgcc ttcaggtact ttcacgcctg taattcatga
     1861 aggatgtttt tatggatcgg atattgggca ggttttcaat tgggtttaag ttgggcggaa
     1921 gtttcttttt catcatcgta cttattgcgg ttaccctgtc tctcagttac tcggacctga
     1981 atcaactgaa tcttggcatg gcgagcatgt atcatgacca taccatcccg attcaaaatc
     2041 tgagtgaggc cagggcattg gtggggcgga tcaagagcaa tattcagctt aatttgcaga
     2101 tccccaaacc cgaggctgaa gatgagaaaa cgaacggagc accgagttgc gattcctgcc
     2161 atgtgttcga gacaagcgga gatcatcatg tgaaggatgg ggaatctgct ggtgatacca
     2221 atcgttgtca ggtgtgtcac tctgcacagt ttgaagattc ggaacatggg ctttcaacag
     2281 ccagtattat gagtgagcaa agctgttcaa gctgccatcc ttctgaagtg atcgacaaac
     2341 agcatcatca ggtggagcag gatattgctg ctgatgttgc acgggtcaat gagatcattg
     2401 cagagtatcg ccagagcggg ttgttgacgg ttgaagagga agcggaactg gcgaggtttg
     2461 acaaggcatg ggcggattat caggtgatca ttactgagat tatttcgcag atggattctg
     2521 gcgaggcgca aactgccttg catcgtgtgg ttggtgggga tgcgttgaag agtcagcagg
     2581 cagtggatga atcgatcggt aagctggtgg ctgttattca ggagcttgcc catcaatcgc
     2641 aggagcaggg cgcaaatacc tttaacactg ctgcacgaga gatgctgtct gtcggtttgt
     2701 ttgccatcct gctttccatt gggttggggt ttgtcatcac agcgaatatt cgtacgcctg
     2761 ttgaggtcat ggctaaaggt ttgcagaata tgcgtcaggg tgatttggat tggaatgtat
     2821 ctgacgcagt gaaggaaagt attgttcagc gttcggatga actgggtgtg gcaggcatgg
     2881 ggtttgatgg cacactacaa tatatgcgtg agatggcagg ggttgcaggc cagatcgctt
     2941 ccggcgacct gaccgttctt gtgaatccgc gtagtgaaaa ggacgaattt ggtctcgctt
     3001 tttcaaaaat ggtggacagt ttaaatgccc tgatcaggct ggtgatggag agcaccgaga
     3061 atctaaattc ggcttccagg caactggctc tctcttccgg acaatctgga gaggcgacac
     3121 gccagatcgc gaccaccatt caacaggtgg caatgggtat tacccaacaa actgccggga
     3181 ttacgaagac atcttcttca gtggagcaga tgaatcgtgc catcgatggg gtggcgcgtg
     3241 gtgctcaggc acaggcggag gcgatcggca aggcttcgct ggttgccggg cgaatcaggg
     3301 ctgccataaa gcaggtgacc agcaacgcgc agaccgtcac ccatgattcg gcggaggcgg
     3361 ccaatcattc gcgcaatggg gccaataccg ttcgggagac cattgcgggg atggaggtta
     3421 tccgttccaa ggtgaatctt tctgctgcca aagtggagga gatgggcgca cgttccgggg
     3481 agatcggcgt gatccttgaa accatagaag atattgccag ccagaccaat ctgcttgcgc
     3541 tcaatgcagc cattgaggca gcccgtgctg gtgaacaagg caagggtttt gcggtggtgg
     3601 ctgatgaggt tcgaaaactg gccgaacgtt cgagcctggc gacaaaagag atcgcgaagt
     3661 tgatcaaggg cattcagggt acggtcaatg aggctgtgga agccatgaat gcttcagcca
     3721 gtgaagtgga ggcaggagtc gtccgcgcca attcagctgg ggtggttttg gagaatattc
     3781 ttgtagcggc tgagtctgtt tacaagcagg ctgaagaagc ggggactgct gctgaaaaag
     3841 tggaagttgc ctccggggag ttggttgagg cggtggatgc cgtctctgcg gtggtggaag
     3901 ataacacagc tgccacagag cagatggcgg gaaattcgtc agagctgacc cagtctattg
     3961 aaaacatggc aagcatcagc gaggaaagca gcgcagctgt cgaagaggtt tctgcgtcca
     4021 ctgaggaggt ctcggtgcag gtgggggagg tctcttcatc ggcagattct ttgatgaaga
     4081 tggcgcagca actttcacag gttgtctcgc gtttcaattt gaaaggctaa tcattatgaa
     4141 aaacgtcgtt cacattccgc tccatcgttc ttttcttggc agactgcttt tcttcatgct
     4201 gctcatcggc attctgcctg tggtcatcaa tgcggccatc agttacaacc tggcacaaaa
     4261 agctttggac gaagccacag atgagatgca gaccattatc gaaaaagacc agacggctta
     4321 tattcttgcc tgggccaatg agcgcaatca ggatatcgta acccttgccg gggtggcccg
     4381 cattacttcc atgaatcccg agactgcgaa tgaagccatc aagcagtact atcgtttgtg
     4441 gggcgggtat gaaaccatct tccttgtgga tatggagggg aaatcaattg ccacatcaga
     4501 cgatcatccg ctggatgtag cggagcggac ctattttcaa gaggcgctta aggggcgggt
     4561 ggtcatgtcg gagccgctca cttccaaggc gacggggaat ttgatcatcg tgtttgcggc
     4621 gcctgtacgg gcgagcggtg gcttgatcgt gggggtggtt ggtgaaacag tgccagtcag
     4681 cgcgatcaca caactattaa ttaagaatag aacgggcgaa acctcggaaa gttatctcgt
     4741 taattcggaa ggatattttg tcactacgcc gcgttttgta aaggaaatgc agaaggcagg
     4801 tttaattgaa ggccagcctg aattgaaata tcaattgcaa acgaccgcca gcaaggaatt
     4861 gcaggcggcc aggtctgggc aggggattta tgaaaactat ctgggtgaag aggtcattgg
     4921 gcgctatacc tggctgcccg acctgaaact gggattgatc tctgaaaagc agacctctga
     4981 agccaaggca gcctcggatc gtcttgccac ttttagtttg attacgatcg ttctttcggt
     5041 tgttctgatc tttgttgtgg cgtttacgat cgcccgcggc attacaagac ctgtgcaatt
     5101 gatggccaat acggccaacc gcctcgccct tggcgatatt gaccagaatc ttgactatgc
     5161 cagcagagat gaatatggca ttcttgccga ctctattcgt ttgatgcttg attatcaaaa
     5221 agaaatggca acggttgcca attccatttc cagcggcaat ttaacagaga gtattcagcc
     5281 gaaatctgaa aaagaccagt tgggacaggc cttcaagcag atgattgaaa gtctgcgtga
     5341 ggccattcag cgtgttgcgt ttagcgcaaa tacattgacc tttgcttctg aacaactggc
     5401 gatctcggca gatcaggcgg gcagggcaac ttctcaaatt gcgacaactg tgcagcaggt
     5461 ggccaaaggc attaccatgg aaacagagtc ggtcacacga acggccttct cggtggagca
     5521 gatgtcacaa accattggga ccgtggcgca gggttcacag gatcagacaa agtctgtaac
     5581 gagtgccacc catattacga accagatcaa tgaagccatt caacaggtgg ctggaaatgt
     5641 gcaggctgta accaacgttt cagctggagc agccgaagca gcccgcatgg gtgcgcagac
     5701 ggtggtgggc acggtgaagg atatgcagtt aatcaagtcc aaggtcaacc tgtcggcaca
     5761 gcgtgtggct gatatgggcg cacagtcaga ccagatcaac gtgatccttg aaaccattga
     5821 agacattgcc agtcagacga atctgctggc attgaatgcc gccattgaag cggcccgtgc
     5881 aggtgaacat ggcaagggat ttgccgtggt agccgatgaa gttcgcaaat tggcggagcg
     5941 tgcaggcagg gccacccgtg agatcggcgg gttgatcaag aacattcagc acacggtttc
     6001 tgaagctgtg atcgccatgg atgaaggcgg tcaggaagtg gagagtgggg tgcaaatggc
     6061 gaatcaggcg ggtaaggcgt tggaagatat tctgaaagct gcagaggaag tgtatcgtca
     6121 ggctgagcag gcagatcacg gcgcggaatt gatgcgatct gcatcgagta gtttggtttc
     6181 atcggtggat gaagtcagca ctgtggtgga ggggaatatg gctgccatgc aatccatgac
     6241 tgctggcatg gctgaaatga cacgcaccat tgaaaatata gctgctgttt ctgaagaaaa
     6301 cagtgccgcc attgaagagg tgagcgcttc ggcggaagag atgagcgccc aagtggagga
     6361 agtcactgct tcggctcaat ctttgatgga aatggcgaaa tcactgcagg agatcgttca
     6421 acaattcaac cttggagagt ctgttgcctc tgcataaatc cgcctgacct ctcactgtcc
     6481 atgttggcgt tgtaagaggg tatcttcggt ttttcatttg gaaaatgctt tttgtaaccc
     6541 tatttggttt gcataatctc ttcaacgatc tcagtaaagg aacgcatcct tccctgctct
     6601 tccaaaggtg ttccaattga gaattttgac atcaactcat ccagattctg aatcgtctca
     6661 gggtcagtct tgttgtgact gcggatcatt tcccacattt caaacgcctt ttccagttca
     6721 ccacgccgcg catagatgcc tcccacccag accaacgcat ccaacgcgat aaagtccgcc
     6781 ttgatgtctt gtgcctcacg gatggattgt ttgatgtaat acaacgcctc gctgtcgttc
     6841 tccaaatgaa aatacgccgt gccgacgtta tccagtgcga tggcaattcc ataccgataa
     6901 cctgtgttgc gatgggtttc gagtccctgt aaaaataatt ccaacgcacc tgcgtagttt
     6961 ttctctttca ataaaatcgt accgagattt cccaacgcgc ccgccaaatt gcgtggttgg
     7021 ttgattcggc gcgagatttc gatggactct tgcaagagcg gcttctcttc ctcgtaacgt
     7081 ccctgattga acaatgccac tgccagattg atgagtgtgg atgaaattcc tgcttcatca
     7141 ttcatctc
//
LOCUS       DAOQLC010000165         7117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4364_length_7117_cov_24.821580, whole genome shotgun sequence.
ACCESSION   DAOQLC010000165 DAOQLC010000000
VERSION     DAOQLC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4364_length_7117_cov_24.821580"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..954)
                     /locus_tag="PL000_19045"
     CDS             complement(1..954)
                     /locus_tag="PL000_19045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HNC91037.1"
                     /translation="MRKGQNPAKFVKDVARPERITVALLNYIPFLSGFYAETLDVLKV
                     SLESMRKDAGLPFDLMVFDNGSCEEVREFLIQEKEEGRIQYLILSEKNMGKGGAWNVM
                     LAGAPGEIIAYTDSDVLFSPTWLSRSVELLEAFPNVGMVTARPYRTSPEFYSSTLEWA
                     RNSEHASLDEGQFIPWETFLEFNLSLGQTVEENEKVYAETKDWRIQYKGMTALAGASH
                     WQFTAYKSTLQKFLPFDMDKPMGQVRQLDKRMNDAGLLRLMVSDPLAMNMSNTLGYLR
                     GELGKERGKKKEGLVRRVLEVGLVKKILLAVYNKIFSWYYS"
     gene            complement(986..1909)
                     /locus_tag="PL000_19050"
     CDS             complement(986..1909)
                     /locus_tag="PL000_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91038.1"
                     /translation="MPLITLFSAPKPFTNPHIAMIQRNAITSWTLLPDVEVILLGEEA
                     GLAEIAKELGVKHLPAVARNANGTPLISSMFQLARENSNGDLLCIINADMVLMPDFVE
                     NAKQTAKLEEKFVLLSQRWDYDITSLLDFSAGWESGLRESVRKQNQLHRPAGSDFFLF
                     PKSCYTDIPNFIIGRAGWDNWMIYKARRENWPVIDCTPSVMIVHQNHDYSHLPGGRSH
                     YDHPDTNENIRLAGGQANIRYTILDATHQLVDGKLTRPKMTSLRFTRRLELVLRSIFF
                     FLPEAMIENIARPKRWQKRFKKLFNVLTFKR"
     gene            complement(1968..2783)
                     /locus_tag="PL000_19055"
     CDS             complement(1968..2783)
                     /locus_tag="PL000_19055"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNC91039.1"
                     /translation="MKPKVVAIIQGRMSSSRLRGKILADIEGQPMLTRVFTRTSRATT
                     LDEVIFATTTDASDDPVAEYCDFSGIPFTRGSLFDVLDRYYQAAKDAKADVVVRITAD
                     CPVIDPELIDRVVNTLLEDEYDFACNRLPPPWTRTYPIGLDVEACTFKVLKKAWKEAK
                     EPQHREHAMPYFYEGVQLTNLNRQLQTGISPRGFNIALLHHTTDFGDYRWTVDTPEDL
                     EFMRQVYVRFDGRDDFTWKEVLDLVHDNPELMKINEGVQHKTLKDVDKRALKR"
     assembly_gap    2900..2999
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3000..>4297)
                     /locus_tag="PL000_19060"
     CDS             complement(<3000..>4297)
                     /locus_tag="PL000_19060"
                     /inference="COORDINATES: protein motif:HMM:NF027579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNC91040.1"
                     /translation="MKPYTSLKSILAEFFPLHRTLASDDHDKTLEIIGSYMPDSSNYT
                     IETYTPLEKAWTWYVPERYAVHEAYLEIEGGERVVDFKNNPLHIVSYSQPVDKTLSFE
                     ELQPHLYFNEKRPHAIPWVFKYYDRDWGFCLPKNVFDKLPRDKKYRAVIKSEFITDPK
                     QGFKMATAVIHPEGGPSASAGEIIVQAHTCHPHQANDDGAGVVSTIELARRLAEKPLP
                     AGSMSVRFWFGPETIGTIVYLSHNEHLIQNLRGGIFMEMTGNKNKIAWHHTRQHTHLL
                     DRITHFIVSKSAHDERDFAAAPANDERVINGPGVNVPCISINRWPYDEYHTTDDNLDI
                     MHDDMLVGAAETAEQIIRMYATNYIPKRTFRGPVFLSGNGLWVDWRENWALNRAIEKI
                     MMRFEGELSVFEIAEQVGLDYWMVRDYIEKFRAKGFVEALP"
     assembly_gap    4298..4397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4398..5539)
                     /locus_tag="PL000_19065"
     CDS             complement(<4398..5539)
                     /locus_tag="PL000_19065"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNC91041.1"
                     /translation="MSANVFVPEDMQQELLRFTQTLVRTKSLSGQEGAVIKLVEKKML
                     DLGYDEVKIDSMGNIIGRIGNGAKSIMFDSHVDTVEVKDEAEWNIPPFSGEIVDGRLH
                     GRGSVDMKSAVAASIYAGALAKQMGFAKDKTIYVSCTVFEEDCDGENLKHLFKELNLK
                     PNYVVICEPSNNIITLGHKGKAQISIKTHGVSAHGSAPEKGVNAIYEMAEIIQRVDKT
                     NLELMKKGTPRGTLVMSQISSVSASLNAVPSECEVYLDRRMIPGETEEDIKREMDEII
                     KGKNATWEVGTLHRKSWTGMDIKYEPFHPAWKIDLEHELAKACISAYQENFGNVPAAY
                     DFWDFSTNAVTPVSMGIPTIGFGPGEYKLAHMRNENCEITQIIDACG"
     gene            complement(5549..5935)
                     /locus_tag="PL000_19070"
     CDS             complement(5549..5935)
                     /locus_tag="PL000_19070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC91042.1"
                     /translation="MTRTSVSSSHAPKAIGPYSQAVWTGNLLYCSGQTPIDPATGKLV
                     AGGISEQTSQAFNNLEAVLTEAGLTMDNVIKVNVYLTSMANFAGMNQVYQERFSMPYP
                     ARTTVAVAELPLNALVEIEFVAEKKQ"
     gene            complement(5950..6708)
                     /locus_tag="PL000_19075"
     CDS             complement(5950..6708)
                     /locus_tag="PL000_19075"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNC91043.1"
                     /translation="MLNKKDLLDGFGKIKIESDTIVVHTSYKSLGGVEGGVDTVIDVM
                     QELVGKDGTVLFPAFNFQSWTETHYFDVMETPSKMGMITEQARLRPNAKRTPHPIYSF
                     SALGKRADEFSKTEDVEAYGPNSAFALFHKLNATIISIGLDFNNSFSMHHYIEYNVGC
                     DYRRIKEFAGIYMGYDRVPQVKTYTMFVRTNDRVKTYIVPGMNDLLHAGVIKEIQVGA
                     AKVHHLTANEFFDNMSVIVREHPEKLHYVEEPKY"
     gene            complement(6698..>7117)
                     /locus_tag="PL000_19080"
     CDS             complement(6698..>7117)
                     /locus_tag="PL000_19080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC91044.1"
                     /translation="KIRQILQSGGVLERVTFGGQISQTDLPRWYHMADLYISPSHVDG
                     SSVSLMEALACGLPCLVSDIPANKEWVFENENGWLFRDGDANNLAEKILAAISQREKL
                     PEIGRASRSTAEMRADWKKNAEALMNVYRSLGGKNAQ"
BASE COUNT         1657 a         1820 c         1673 g         1767 t
ORIGIN      
        1 ctacgaataa taccaactga agatcttgtt atacaccgcc agcaaaatct tcttcaccaa
       61 accaacctcc agcacacgcc gcaccaatcc ttctttcttc tttcctcttt cttttcccaa
      121 ctcccctctc aaatacccca gcgtattgga catattcatc gccagcggat cactgaccat
      181 caagcgcagc agacccgcgt cattcatacg cttatccaac tggcgcacct gtcccattgg
      241 tttgtccatg tcgaagggta aaaacttttg cagtgtggat ttatacgccg tgaactgcca
      301 atgacttgca cctgcaagcg cagtcatccc tttgtattga attcgccaat cctttgtctc
      361 tgcgtagacc ttttcattct cctccacagt ttgtccaagc gaaagattga actctaaaaa
      421 tgtttcccac ggaatgaatt gcccctcatc cagggatgca tgttctgaat tcctcgccca
      481 ctccagcgtg gaagaataga actcaggcga agtgcggtat gggcgcgccg ttaccatgcc
      541 gacatttgga aaggcttcga gcaattccac cgaacgcgag agccatgtcg gcgaaaacaa
      601 aacgtcacta tcggtatagg caatgatctc accgggcgcg cctgccagca ttacattcca
      661 tgcgccgccc ttgcccatgt tcttctcgga gaggatcaaa tattgaattc gtccctcctc
      721 cttctcctgg atcaaaaact cgcgcacctc ttcacaggaa ccgttatcga agaccatcag
      781 gtcaaacggc aaccctgcat ccttgcgcat ggattcgagt gagaccttca gcacatccaa
      841 agtctcggcg taaaatccgc tcaggaaggg aatgtaattt aacaacgcca ccgtgatccg
      901 ttcggggcgg gcaacatctt tgacgaactt ggcagggttt tgaccttttc gcatgttagg
      961 ttccaggttg gaaagttgga acgttttaac gtttgaacgt taaaacgtta aataatttct
     1021 taaatcgttt ctgccagcgc ttcgggcggg caatgttttc gatcatggct tcggggagaa
     1081 aaaagaaaat agaacgcaag accagttcaa ggcggcgtgt gaagcgaagc gatgtcatct
     1141 ttgggcgggt aagtttacca tctacaagtt gatgggtcgc atccaagatc gtgtatcgga
     1201 tattcgcctg tccgcctgcc agacggatat tctcattcgt atcgggatga tcataatgcg
     1261 acctgccccc gggcaaatgg ctgtaatcgt gattttgatg cacgatcatc accgaaggtg
     1321 tgcaatcgat cacgggccag ttttcgcggc gggctttata gatcatccag ttatcccaac
     1381 ctgcacgacc gatgatgaaa tttggaatat cggtatagca ggattttgga aaaaggaaaa
     1441 aatcgcttcc agcgggacgg tgtagctgat tctgttttct gacagattct ctcagcccag
     1501 actcccaccc cgcagaaaaa tcgagcagcg atgtgatgtc gtaatcccaa cgttgactca
     1561 acagcacaaa cttctcttcc aactttgccg tttgttttgc gttctcaaca aaatcaggca
     1621 tgagaaccat atcggcattg atgatgcaaa gcaaatcacc gtttgaattc tcccgcgcca
     1681 gttgaaacat ggatgagatc aacggtgtgc cgtttgcatt gcgggcaaca gcaggcaaat
     1741 gcttcacgcc cagttctttt gcaatttcag caaggcccgc ttcctcacca agcaaaatga
     1801 cctccacatc agggagcaga gtccacgatg tgatggcgtt gcgttggatc atcgcgatat
     1861 gcggattggt gaaaggcttt ggagcggaaa ataaagtaat tagaggcatg ggggaggttt
     1921 acaggtttga aggttgaaac ttgcgagctt tccaaacttc aaactttcta acgcttgagt
     1981 gcgcgtttat caacatcttt caaagtctta tgctgcacac cctcgttgat cttcatcaat
     2041 tcgggattgt cgtgaacaag atccaaaact tccttccagg taaagtcatc gcgtccatca
     2101 aaacggacat agacctggcg catgaactca aggtcttcgg gtgtatccac tgtccagcgg
     2161 taatcgccaa aatcggtggt gtggtgaaga agagctatgt taaatccgcg tggggaaatg
     2221 cctgtttgca gttggcggtt gagattcgtc agttgcacac cttcatagaa ataaggcatg
     2281 gcatgttcac ggtgttgggg ctctttggct tccttccagg cttttttcaa aaccttgaaa
     2341 gtgcaggcct ccacatccaa cccaatggga taggtacgag tccacggcgg cggcagacgg
     2401 ttacaggcaa aatcatattc gtcttcgagc aaagtattga ccacacgatc gatcagctct
     2461 gggtcaatga cggggcaatc tgccgtgatg cggacaacca catcggcttt ggcgtctttg
     2521 gcggcctgat aatatctgtc cagcacatcg aacaaactac cgcgggtgaa gggaatgccg
     2581 ctaaagtcgc agtactcagc cacggggtcg tctgaagcgt ccgtcgtagt ggcaaagatg
     2641 acttcgtcga gagtcgtcgc tcgagaggta cgggtgaaga cacgggtcag catcggctgg
     2701 ccttcaatgt cggcaaggat cttccccctc agtcgagaag acgacatgcg gccttgaatg
     2761 atggcaacta cttttggttt cataattttt tgtaggggca cagcggcgct gtgctcctac
     2821 gcactttgcg cctcggatgg aataggcaac gcttcaacga atcccttggc gcggaatttt
     2881 tcgatgtaat cgcgcaccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     3001 gcaacgcttc aacgaatccc ttggcgcgga atttttcgat gtaatcgcgc accatccagt
     3061 aatcgagccc gacctgttca gcgatctcaa aaaccgacaa ctccccttca aagcgcatca
     3121 tgatcttttc gatggcgcgg ttgagtgccc agttctcacg ccaatcgacc cagaggccat
     3181 tgccgctgag gaacacaggt ccgcggaagg tgcgtttggg aatgtagttc gtcgcataca
     3241 tgcggatgat ctgctcagcg gtttcggccg caccgaccag catgtcatca tgcatgatat
     3301 cgaggttgtc atcggtggtg tgatactcgt catagggcca gcggttgatc gaaatgcaag
     3361 gcacgttcac gccaggcccg ttgatcacgc gctcgtcatt ggcgggggcg gcagcaaaat
     3421 cacgttcatc atgtgctgac ttgctgacaa taaaatgcgt gatgcggtca agcagatgcg
     3481 tatgctgacg ggtatgatgc caggcgatct tgttcttgtt ccctgtcatc tccataaaga
     3541 tgccaccgcg cagattttga atcagatgct cgttgtgcga gagataaacg atggtgccaa
     3601 tggtctcagg tccaaaccaa aagcggacgg acattgagcc cgcaggcagc ggcttttcag
     3661 cgagacggcg cgccaattca atggtgctga ccacacctgc accatcgtca ttggcttgat
     3721 gcgggtgaca ggtatgcgcc tgcacgatga tctcacccgc tgaagcgctc ggtccgcctt
     3781 cagggtgaat gacagcggtt gccattttga atccctgttt cgggtcggtg atgaattcag
     3841 atttgatcac tgcgcgatat tttttgtcac gcggcagttt atcgaagacg tttttgggca
     3901 ggcagaagcc ccaatcgcgg tcataatact tgaacaccca cggaatggca tgtgggcgtt
     3961 tttcgttgaa atacaaatgc ggctgaagtt cttcgaacga aagtgttttg tccacgggct
     4021 gggagtacga aacgatatgc aatggattgt tcttgaaatc caccacacgc tcgccgccct
     4081 cgatctcaag atacgcttca tggacggcgt aacgctcagg cacataccat gtccatgctt
     4141 tttcaagcgg agtgtaggtt tcgatggtgt aattggacga atcgggcatg taactgccga
     4201 tgatctccaa agtcttgtca tggtcatcag aagcaagggt gcggtgcagg gggaagaact
     4261 ccgcgaggat ggatttgagg gatgtgtagg gtttcatnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnccg catgcgtcaa tgatctgagt aatttcgcaa ttctcgttcc
     4441 gcatgtgggc gagtttatat tctccagggc caaaaccgat ggtgggaatg cccatactga
     4501 caggcgtcac cgcattggtg ctgaaatccc agaagtcgta tgcagcaggg acgtttccaa
     4561 aattttcttg atacgcagag atgcaagcct ttgccagttc gtgttccaag tcaattttcc
     4621 atgccgggtg gaaaggttcg tacttgatat ccatgcccgt ccagcttttg cggtggagcg
     4681 tgcctacttc ccatgtggcg ttcttgcctt tgatgatctc atccatctcg cgcttaatgt
     4741 cttcttccgt ttcgcctggg atcattcttc tatcgagata cacttcgcac tcggagggga
     4801 ctgcgttcaa tgaagcactg acgctggaaa tctgcgacat gaccagtgta ccgcgtgggg
     4861 tgcctttttt catcaactcg agattggtct tgtctacacg ttgaatgatc tcagccattt
     4921 catagatcgc gttcacgcct ttttccggtg cagagccgtg cgcagaaacg ccatgcgttt
     4981 tgatggaaat ttgcgccttg cctttatgtc ccagcgtaat gatgttattc gatggttcgc
     5041 aaatcaccac ataattgggt ttgaggttga gctccttgaa caagtgcttg aggttttcgc
     5101 catcgcagtc ttcttcaaag acagtacagg agacatagat tgttttatct ttggcgaaac
     5161 ccatttgttt ggcaagcgcg cctgcataaa tggacgcagc cactgcggac ttcatatcca
     5221 ccgaacccct gccgtgtaaa cgcccatcca cgatctcgcc gctgaatggc gggatgttcc
     5281 actcagcctc atccttgact tccaccgtgt ccacatgcga atcgaacatg atggacttcg
     5341 cgccattccc aatgcgcccg atgatatttc ccatcgaatc aattttgacc tcatcgtagc
     5401 cgaggtcaag cattttcttt tcgacgagct tgatgacagc gccttcctga cctgaaaggc
     5461 ttttcgtcct caccagtgtc tgggtaaagc ggagcaactc ctgttgcatg tcttcaggca
     5521 caaaaacatt cgcggacatg gtttcctttt actgtttttt ctcagcgacg aattcgatct
     5581 caaccagtgc attcaaaggc aattcggcaa ccgcaacggt cgtgcgggca ggatacggca
     5641 ttgaaaaacg ctcctgatag acttgattca ttccagcaaa gttcgccata ctggtcaaat
     5701 acacattgac cttgatgacg ttgtccatgg tcaggcccgc ctcggttaat accgcctcaa
     5761 gattgttgaa agcctgcgaa gtctgctcgc taatgccgcc agccaccaac ttccctgtgg
     5821 cagggtcaat tggggtttgg cccgagcaat acaacaagtt ccccgtccac acagcctgcg
     5881 aatacggacc gatcgccttg ggtgcatgag aactcgatac agatgtacgc gtcatttttt
     5941 ctccaacact taatatttcg gctcttccac gtagtgcaac ttttcagggt gttcgcgcac
     6001 aatgacagac atgttatcaa agaactcatt cgcagtcagg tgatgcacct tcgccgcgcc
     6061 aacttgaatt tctttgatca cacccgcgtg caaaagatca ttcatccccg gcacaatgta
     6121 agtcttcacg cggtcatttg tgcgcacgaa catcgtgtat gtcttcacct gcggcacacg
     6181 atcatagccc atataaatgc ccgcaaactc cttgatgcgg cggtaatcac agcccacgtt
     6241 atattcaata taatgatgca tcgaaaacga attattgaaa tcaagcccaa tgctgatgat
     6301 ggtggcgttc aacttatgaa aaagcgcgaa agccgaattc gggccatagg cttccacatc
     6361 ttcggttttc gaaaattcgt cggcgcgttt tcccaaagcg gagaaactgt agatcggatg
     6421 cggagtccgc tttgcgttag gtctgaggcg cgcctgttcc gtgatcatgc ccatcttcga
     6481 aggcgtctcc atcacatcga agtaatgtgt ctccgtccac gattgaaaat tgaacgcagg
     6541 gaacagcacc gtaccgtcct tccccaccaa ctcctgcatc acatcgatca ccgtatccac
     6601 gccgccctcc acgccgccca gcgatttata cgacgtatgc acaacgatcg tatcactttc
     6661 aatttttatc tttccaaaac catcgagcaa atctttttta ttgagcattt tttcctccga
     6721 gacttcgata gacattcatc aacgcttcgg cattcttttt ccaatccgcg cgcatctctg
     6781 ccgtgctgcg gcttgctctg ccgatctcgg gcagtttctc cctttgagag atcgccgcca
     6841 gaatcttttc ggcaaggtta ttcgcgtccc catcgcggaa cagccagccg ttttcatttt
     6901 caaagaccca ttccttgttg gcagggatat ccgaaaccag acaaggcagc ccgcaagcca
     6961 acgcctccat cagtgaaacg gacgagccat ccacatgtga aggtgaaata tacaggtcag
     7021 ccatatgata ccagcgcggc aggtcggttt gtgagatctg cccgccaaag gtgacccgtt
     7081 ccaagacacc gccgctctgt aagatctgac gaatttt
//
LOCUS       DAOQLC010000166         7114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4370_length_7114_cov_24.683808, whole genome shotgun sequence.
ACCESSION   DAOQLC010000166 DAOQLC010000000
VERSION     DAOQLC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_7114_cov_24.683808"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PL000_19085"
     CDS             <1..516
                     /locus_tag="PL000_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91045.1"
                     /translation="QNLVWLFGILSTALLGLYWWSHAMDWSWWQYATALLLALDVLGG
                     VVANSLNSCKRFYHSPIQHEETGFTALAKNHFAFTLLHIHPILIGLVFGNMNLAYGII
                     WYVVMVIVSAGVQKLPLYLQRPTAMGVIMLAILINYYVIPPITGFEWFIPALFLKIVY
                     GHIVQEEPYRA"
     gene            complement(530..2197)
                     /locus_tag="PL000_19090"
     CDS             complement(530..2197)
                     /locus_tag="PL000_19090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC91046.1"
                     /translation="MSEKKNQPDMQRILSISQELVADRPLDEILHRIVQVAADLVNCE
                     SVGILLSDESANTLRFVAATLYQDRLFDFPVPIDASIAGAAFISNELVNVPDVSVDPR
                     YYPKIADLLNFPARSLLAAPLKFKDRKIGVLEAQNKKDREEFDAVDVQIMTALAAQVS
                     IALEHARQMERYKQLAQDEQNQRQMAEAMRLASAALTSTLDYDQVIDNIMEQVSSVIP
                     LDTCNVMMIEKEDTARIARVYRGRGYEQVGTAATLQDTTLNISDVAGLRRMCETRQPV
                     LIFDVAQDLEWVYSRPEHHWIRSYIGAPIIIRDSVVGFLNVMSGTPNLYNQALAERLQ
                     AFVHYAATAIENARLYQQAQEEIAERIKAEEELRHHRDHLEELVKERTADIHRLAITD
                     SLTELFNRRHLISLGERAINLAQRYHHPLVAMMADLDHFKKINDTYGHAAGDEALRKL
                     AAQIRQVFRSTDILGRYGGEEFVVLMPETDIVSGHKIAERLLHLTQGVSISSDKIEFG
                     FTISIGLAERSQTNARTLDQLIDHADSALYNAKQSGRNRVEVYQVPR"
     gene            complement(2278..6234)
                     /locus_tag="PL000_19095"
     CDS             complement(2278..6234)
                     /locus_tag="PL000_19095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC91047.1"
                     /translation="MNILYTYIPQDRLRALAHGEDLPTHTSGSALFADISGFTPLTRA
                     FAEAFGPKRGAEALLGVLNPLFEALIEPVHRFGGSVIGFAGDAITCWFEASPRHSEPS
                     LYHSSLRATAVALAMQSVMKPFAAVHAPDGSLVTLSIKVAIAAGSARRFMVGDPAIQQ
                     MDTLAGITLSRMAEAEKYASQGEVVVSGEVAESLKDVLNVSAWRDEQRYAVVDGLKVE
                     VQAEPWPILTEDALSEVQVRQWVLPFIFEQLNSGVVNLGDLRPVTPLMVQFEGIDFDA
                     DENAGSKLDSFVSWSQRIVHQYGGSLIQLTIGDKGAFMYAVFGAPLAHENDPTRALQA
                     ALALRDMPVDLAAYLKPLRIGLTYGMAWTGNYGGYERFTYGVISSEVNLSARLMGKAE
                     PGQILVSGRMGQYPGFRFEHVGNLIFKGFDTPLPTYSLLGEQVSDAQAFQTAMVGRDD
                     ELEKLATFAQPLFANHLAGVAIVYGEPGIGKSRLAYALHQMFGERVSWFTGQTDQILS
                     QAFNPFIYWLKRYFDQSPNASMEQNKVNFESRLDQLGDSLQALTVSPHSPISNLQSEL
                     IRTRSFLGALLGFDWPDSLYAQLDSPKLRYENTLIAIKTLLLAESQLRPVVFELEDGH
                     WLDDPSREMLLAISRNVAAYPLLVLITSRYNDDGSQPVFALDREIPILKLDLEALTRE
                     ALERQAAAVLGGKISPALLALLWERSRANPFFAEQMLLHFHETGILIQTQMGEWEFQS
                     SPVTLSIDVHTMLVARIDRLTHKVRQVVQAAAVLGIEFDVQLLSKMLHTDALPEVQDA
                     ERGQIWQLMRELRYIFKHALLRDAAYDMQLRSRLRELHHLAAVTAEQVYEDQLSSYYD
                     ILAYHYRMAYQLGVENVSEQVCFYLRKAGEAAQKNFANSVALDYYNQLLPMLQDLSEQ
                     AQVYLQRGAVLELIGRWPEAENDYRTALDLVDGNTGMQAGAQLALGKLFHLRGDFSPA
                     LKWFEQAQMTWTTLQDQAGLSQTLIATGFVLWRKGDLQDARRYLEEGLTLARMLHDQP
                     GVALALEGLGRVAHVQDDYAVSQALHTESLSLRRELGDKQGIAWSLNYLGIVAAGQGD
                     YVVAQALFEESLALFRDLGDKFGIAEVLEKLGITMRFRGEWGKARVFYEESLDISREI
                     GDRSSTAWVLYNLGAVAHSQKEYETARALLEEGLVHFRELGHKAGIAWVLSFLGKPLT
                     NLQQFSAARAAFREALVVFQQLQSTIGVIFSIEGVVNLSIAQGQLEESAQLIAWATAA
                     RESINNPRPVGEQEETDLALANLRTALGEDTFHTAWMEGGRMTMEEAIQLALHDVP"
     gene            complement(6556..>7114)
                     /locus_tag="PL000_19100"
     CDS             complement(6556..>7114)
                     /locus_tag="PL000_19100"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNC91048.1"
                     /translation="LVEQTDIADLQNRLAQTDNADIQLVYTNLMNASYNHLAAFTGEQ
                     GGNGQQGGNGNGNGGGNGQAGQAGQGQVGSGVPQANISGAVTVHGVVNSYDGMGISFT
                     ADDGQVLYVDTGNMRYSQSIGFAPVSGDGMTVVMFLGDQGTNSAISVTVDATGMTYTF
                     RNETGQPLWVGGNGSGNGNGNGGNH"
BASE COUNT         1804 a         1949 c         1599 g         1762 t
ORIGIN      
        1 caaaatctcg tctggctgtt tggcattctt tctactgctc tgcttggctt gtactggtgg
       61 agccacgcaa tggactggtc ttggtggcaa tatgccactg ccctcctgct tgcgttagac
      121 gtgctaggcg gcgtggtcgc caactcgctc aacagttgca aacgctttta tcactccccc
      181 attcaacatg aagagacagg ctttacagca ctggcaaaga accattttgc attcactctc
      241 ctccacattc accctatcct cattgggctt gtgttcggaa acatgaatct ggcttatggc
      301 atcatctggt atgtagtcat ggtcattgtt tccgcaggag ttcaaaagtt gccgctatat
      361 cttcagcgcc ctacggctat gggagtcatt atgctggcaa ttttgatcaa ttattacgtg
      421 atcccaccta taacaggctt cgagtggttc attcctgcat tgtttcttaa gatcgtatac
      481 gggcatattg tgcaagaaga accctatcgc gcttaatgca aacggtattt caccttggaa
      541 cttgatacac ctcaacccgg ttgcgtcctg attgcttggc gttgtacaag gcactatcgg
      601 catgatcaat aagttgatcg agcgttcgag cattggtctg agatcgttct gccaggccaa
      661 tgctaatggt aaatccgaat tcaattttgt cactgcttat actcacccct tgggtcaaat
      721 gcagcaaacg ttcggcgatt ttatgtccac tgacaatatc cgtttcaggc atcagaacaa
      781 caaactcctc cccgccataa cgtcctaaaa tgtcagtact gcgaaagacc tgacgtattt
      841 gagcagccag tttgcgcaag gcctcatccc cagcagcatg tccataggtg tcgttgatct
      901 ttttaaagtg atcgaggtct gccatcatgg ccaccaaagg atggtgatag cgctgtgcaa
      961 gattgatggc ccgttccccc aaactgatga gatggcggcg gttgaaaagt tcggtaagcg
     1021 aatcggtaat tgcaagacgg tgaatatcgg ccgtccgctc cttgaccaac tcttcaagat
     1081 gatcacgatg atggcgcaat tcctcctctg ccttgatccg ttcggcgatt tcctcctgtg
     1141 cctgttggta caaccgcgca ttttcaatgg cggtagccgc ataatgaaca aaagcctgta
     1201 atcgttcagc aagagcctgg ttgtacaaat ttggtgttcc gctcatgaca ttgagaaaac
     1261 cgaccaccga gtctcgtatt atgatgggtg cgccaatata cgatcggatc caatggtgtt
     1321 ccgggcgcga atagacccat tccaaatcct gtgccacatc aaagatcaag acaggctgac
     1381 gggtctcaca catacggcgc aaaccagcta catccgagat gttcagggtg gtgtcttgca
     1441 gagtggcggc agtgccaacc tgttcataac cgcgcccgcg atatacccgg gcaatacggg
     1501 cggtatcttc tttttcgatc atcatcacat tgcaggtatc caatggaata acagaactga
     1561 cctgctccat gatattgtcg atcacctggt cataatccag tgtactggtc aatgccgcgc
     1621 ttgccagccg catggcttcg gccatttgcc gctggttctg ttcatcctga gccagttgtt
     1681 tgtagcgttc catctgccgc gcatgttcaa gcgcgatgga tacttgcgct gcaagtgccg
     1741 tcatgatctg gacatcaact gcatcaaatt cctccctgtc ctttttgttt tgcgcttcca
     1801 aaacaccaat tttgcgatct ttgaatttga gtggagcagc cagaagagaa cgcgccggga
     1861 aattgagcag gtcggcaatt ttaggataat agcgcggatc aacactgaca tccggtacgt
     1921 ttaccaattc attgctaata aacgccgccc cggcaataga cgcgtcaatg ggcactggaa
     1981 aatcaaaaag ccgatcctga tacaaagtcg cagcgacaaa gcgcagggta ttcgcagatt
     2041 catccgacaa aagaatgcca accgactcac aattgaccag gtcagctgca acttgaacaa
     2101 ttcgatgcag gatctcatca agtggacggt ctgcaacaag ttcctgactg atcgacagga
     2161 tccgctgcat atcaggctgg ttcttttttt cagacatgag ggaaattata cgccctaaaa
     2221 ccttccaata tatcagggga tatagatgac ctttcaaaaa tcaaacacat aaatagatca
     2281 tgggacatca tgcaaggcca attggatggc ttcttccatc gtcatcctcc cgccttccat
     2341 ccaggctgta tgaaaggtgt cttcacccag tgcagtgcga aggtttgcaa gtgcaaggtc
     2401 ggtttcttcc tgttcaccga cggggcgtgg attattaatg gattctcgtg cggcagtggc
     2461 ccaggcaatc aattgggcgg attcttctaa ttgtccctgc gcaatggaca aattcaccac
     2521 accctcgata gaaaaaatca caccgatggt gctttgcaat tgctgaaaaa caaccaacgc
     2581 ctcacggaaa gctgcacggg ccgcactgaa ttgctgcaaa tttgtcagtg gtttacccag
     2641 aaaagagagc acccaggcaa tacccgcttt atgacccaac tcacgaaaat gtaccagacc
     2701 ttcttccaac aaggcccgtg ccgtttcata ctccttttgg ctgtgtgcca ccgcacccag
     2761 gttgtatagc acccaggctg tggatgaccg atcacctatt tctctgctaa tatcgaggct
     2821 ctcttcataa aatacccgtg ccttgcccca ttcccctcga aaccgcattg ttatccccaa
     2881 tttttcgagc acctctgcaa tgccaaactt atcacccaaa tcacgaaaaa gggccagact
     2941 ctcttcgaat aatgcctgcg caacaacata gtcaccctga ccagcagcca caattcccag
     3001 atagttgagc gaccacgcaa tcccctgctt atcgcccagt tctcggcgga gtgacaggct
     3061 ctctgtgtgc aacgcctgtg acactgcata gtcatcttgg acgtgtgcca ccctccctaa
     3121 accttcgagt gccagggcta caccgggttg gtcatggagc atccgtgcca atgtgaggcc
     3181 ttcttccaaa taccgacggg catcttgaag gtcacctttg cgccataaga caaagccggt
     3241 tgcaatcagt gtctggctca aaccagcctg atcctgcagc gttgtccatg tcatctgtgc
     3301 ctgttcaaac catttcagcg caggggaaaa atcgccgcgc aggtgaaaca acttccccag
     3361 cgccaattgg gcccctgctt gcatacccgt gttcccatcc accaaatcca atgctgtccg
     3421 atagtcattt tcagcctcgg gccatctgcc gatcagttcc agtaccgccc ctcgttgcaa
     3481 atacacttgt gcttgttcgg ataaatcctg aagcatgggt aatagttgat tatagtaatc
     3541 cagtgcaaca gagttggcaa aattcttttg tgctgcctcg cccgccttgc gcaaataaaa
     3601 acatacctgc tcagacacat tctccacccc caattgatat gccatgcggt aatgatatgc
     3661 caggatgtca taataggatg acagttggtc ttcatatact tgttctgcag taactgctgc
     3721 cagatgatgt aactctcgca gacgggaacg taactgcata tcataggctg catcccgcaa
     3781 aagcgcatgt ttgaagatat atcgcaattc gcgcattagc tgccagattt gcccgcgttc
     3841 cgcatcctgg acctcgggca gggcatcggt atgcaacatc ttcgagagca attgcacatc
     3901 gaactcgatc cccaacacag cagcggcttg caccacctgc ctgaccttgt gtgttaaacg
     3961 gtcaatgcgt gccaccagca ttgtgtgaac atcgatggac aaagtgacag gcgaagactg
     4021 gaattcccat tcacccatct gggtttgtat caatatgccc gtttcgtgaa aatgcagcaa
     4081 catctgttct gcaaagaagg gattggcgcg actcctttcc cacagtaaag ccaacaaagc
     4141 tggggatatt tttcctccca agacggcagc agcctgccgt tccaatgcct cacgagtaag
     4201 tgcctcaaga tcaagtttga gtatggggat ttcccgatcc aaagcaaaga caggctgact
     4261 gccatcatcg ttatagcgtg aggtgatcag aacaagcaga gggtatgcag ccacattacg
     4321 gctaattgca agcaacatct cacgcgaagg gtcatccaac cagtgcccgt cttccaattc
     4381 aaatacaaca ggtcgcagct ggctttctgc caacaacagt gttttgatgg cgataagcgt
     4441 attttcgtag cgtaattttg ggctgtccaa ttgtgcataa agtgaatcag gccaatcaaa
     4501 gcccaacaat gcacctagaa aggaccgcgt acggataagt tcagattgca aattggagat
     4561 tggcgagtgg ggcgaaacgg tcaatgcttg caggctatcg ccaagttgat cgaggcggct
     4621 ttcaaaatta actttattct gctccattga agcgtttggc gattgatcaa agtaccgctt
     4681 aagccagtag atgaaggggt taaacgcctg actcaagatt tgatcggttt gcccggtaaa
     4741 ccaggaaacc cgttcgccga acatttgatg caatgcatac gccagacgcg atttaccgat
     4801 gcccggctca ccatagacaa ttgccacgcc cgccaagtga ttcgcgaaga gcggctgggc
     4861 aaatgttgca agtttctcca gttcgtcatc gcgccctacc attgctgtct gaaaagcctg
     4921 tgcatccgat acctgctcac ccaacaaact gtatgttggc agcggggtgt caaagccctt
     4981 gaaaatcaga ttccctacat gctcaaaacg aaagcccgga tattgaccca ttcgccctga
     5041 aaccaaaatt tgtcctggct ctgccttgcc catcaggcgt gctgagaggt ttacttcact
     5101 gctaataacc ccataggtga accgctcata tccgccataa ttccccgtcc atgccattcc
     5161 atatgttaaa ccaatgcgca atggtttgag gtaagcggcc aggtccaccg gcatatcacg
     5221 caaggccaaa gccgcttgta aagcccgcgt tggatcattt tcgtgtgcaa gaggtgctcc
     5281 gaaaacagca tacataaacg cacccttgtc gccgatcgtc aattggatca aactgccgcc
     5341 atactgatga acaatacgct gcgaccaact cacaaacgaa tccaacttac tcccggcatt
     5401 ctcatccgca tcgaagtcaa tgccttcaaa ctggaccatt agaggtgtca ctgggcgtaa
     5461 atcgcctaaa ttgaccacac cactattcag ttgttcaaag atgaatggca acacccattg
     5521 acgaacctgc acttcgctca atgcgtcctc tgtcaatatc ggccagggtt ctgcctgaac
     5581 ctccaccttc aaaccatcga ccaccgcata ccgctgctca tcgcgccatg cactgacgtt
     5641 caggacatct ttcaagctct ctgcaacttc cccactgact acaacctcac cctgactggc
     5701 atatttctct gcctccgcca tccgtgataa tgtgatgccc gccagggtat ccatctgctg
     5761 aattgctggg tcacccacca taaaacgtcg cgcagaacca gccgctatcg cgactttgat
     5821 gcttaacgta actaatgaac catcaggtgc atgtacagca gcaaatggtt tcattactga
     5881 ttgcatcgcc aacgcaacag ccgttgcccg cagggaggaa tgataaaggg atggttcaga
     5941 atgccttgga gaagcctcga accagcaggt aattgcatca cccgcaaaac cgatcacact
     6001 gccgccgaag cgatgcactg gctcgatcag cgcctcgaac aacgggttaa gcacacccaa
     6061 tagggcttct gcaccacgct tggggccaaa cgcttcagca aaggcacggg tcaatggagt
     6121 gaaaccagaa atgtccgcaa acagggcgct gcccgatgta tgggtgggca gatcctcgcc
     6181 atgggcaagt gcgcgcaaac ggtcttgtgg aatataggtg tacaggatat tcatgggttt
     6241 cctgagtagg gatttcgttt cagtaattgt tcaataaatt gggaagcatg tcaagttaat
     6301 atatttcttt atacccaatg atttgtttaa cactgaaacg caacggctta aatcgccaaa
     6361 tctcaacaag gttttcccat gaaaatatcc tctaccacca tctaaacgaa tccaaatatc
     6421 atcctggtga tgtaatctct cacaatattg gttgccgcaa gattgatatt gcctaactat
     6481 ttgcaataaa aacgcccctg ccgaagcaga ggcgttttta ttggttttag tagagcgcca
     6541 tacttttaca tctgattaat gatttcctcc attcccattc ccattcccac tcccattgcc
     6601 acctacccac aacggctgac ccgtttcatt gcggaaggtg tatgtcattc ctgttgcatc
     6661 caccgtgacc gagatcgcgc tgttcgtgcc ctggtcaccg aggaacatga ccaccgtcat
     6721 accatcgcca gacacaggtg cgaacccaat ggactggctg tagcgcatgt tgcccgtgtc
     6781 cacgtagaga acctgtccat catcggcggt gaagctgatg cccatgccgt cgtatgaatt
     6841 gaccacaccg tggactgtga ctgctccaga aatattcgcc tgcggaacac cgcttcccac
     6901 ctgaccttga cctgcttgcc cagcctgacc atttcctccg ccgttgccgt ttccattgcc
     6961 gccttgttga ccattgccac cctgttcacc cgtgaaagcc gcaaggtgat tgtacgaagc
     7021 gttcatcagg ttggtgtaca ccagttgaat atcggcattg tcagtttgag caaggcgatt
     7081 ctgcaagtct gcgatatcgg tctgctcaac caat
//
LOCUS       DAOQLC010000167         7094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4391_length_7094_cov_13.298054, whole genome shotgun sequence.
ACCESSION   DAOQLC010000167 DAOQLC010000000
VERSION     DAOQLC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4391_length_7094_cov_13.298054"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..921)
                     /locus_tag="PL000_19105"
     CDS             complement(325..921)
                     /locus_tag="PL000_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91049.1"
                     /translation="MKARDMKFTPGMEMHIEKLLHSRMTSILSDRVLMRIWQRLGGEV
                     FRRSSVLYGLDEFLTRCKVKGGTVVEIGTRHGLTAVVLARHFDHVITMDVEKSTYAQE
                     VFSLADVSHKVMHLVIGSNEEKKEILNHHLVFDFAYLDGNHADDTMLDWGLTKKCGRV
                     LFQEAWEWQKPVWDLVRSLPQDEVRYFPGSSFALWMKK"
     gene            complement(918..1232)
                     /locus_tag="PL000_19110"
     CDS             complement(918..1232)
                     /locus_tag="PL000_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91050.1"
                     /translation="MSPERFTQILDRRIELIRANLKSKEYASDYDRLHNFKRAAELLR
                     ELPAESCVGFMTKHIVSVLDLVRNHANGDDISRAQIDEKIGDAINYLILLEAIFLEDD
                     AP"
     gene            complement(1229..1435)
                     /locus_tag="PL000_19115"
     CDS             complement(1229..1435)
                     /locus_tag="PL000_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91051.1"
                     /translation="MNAIKGSCENCGAGFHRVADSGDALMIDGVYHQEYIWCKLHPKP
                     VAKSKHDFCFQWREQEIDERKVAP"
     gene            complement(1432..1692)
                     /locus_tag="PL000_19120"
     CDS             complement(1432..1692)
                     /locus_tag="PL000_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91052.1"
                     /translation="MLIRGCCEDCVFARPKNENDSGQFSTLVCYFMPQPVEKLPQEFC
                     AQFSSAKDIHVESEFVLDTSGLEKKTLIRLADAIKKEFGDKL"
     gene            complement(1686..2252)
                     /locus_tag="PL000_19125"
     CDS             complement(1686..2252)
                     /locus_tag="PL000_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91053.1"
                     /translation="MSKAPASAVAGKIGKRKGEQAFLREWKKSVLSHPRSFWYKIPDI
                     FPMGEMRWIPQKPFDCIFAIDEIVFFAEAKFSNDCEHAWSFDNVSDHQAENLDLASRA
                     GVPSGILLNVRQGLGSRRYNKTFAIGINAFNELRAKKKSIRPGELERLVPFAVPEGVI
                     WPIRGELLRDAFNLKKPVDSAVQEFAFC"
     gene            complement(2249..2551)
                     /locus_tag="PL000_19130"
     CDS             complement(2249..2551)
                     /locus_tag="PL000_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91054.1"
                     /translation="MEKFDLATVLRGEFLEKGNKKIAQAVRHCKKYSENTQIRKVMLE
                     ASIFPEAGTGDIIIQGDVKISLPGMKDSERFILAKKLVEKNGQLTFESKKDPKVSK"
     gene            complement(2656..3168)
                     /locus_tag="PL000_19135"
     CDS             complement(2656..3168)
                     /locus_tag="PL000_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91055.1"
                     /translation="MGEKDFYTAGAVAGIVQLPLEECKFSQCMDCGAWRGTFYHRGGI
                     VEKYVIHHPKCKACARTNFYTEDLKKFAAVHGLIFQTKNEKEKTKEVVTMKVFRFTLF
                     KAVYTPSVSDSTKSEITSFEARETFDVVAGDLPQALMSLDVEGYMKKNAITNRALVHV
                     AQEVLELKSL"
     gene            complement(3212..3733)
                     /locus_tag="PL000_19140"
     CDS             complement(3212..3733)
                     /locus_tag="PL000_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91056.1"
                     /translation="MLRLHHFQSDQIFFPSLLRFSARWEWCRPFMSGKFSVVWSASIR
                     SGKLTITYRDDFAEYVRKMRDGEYELILRKKKRDAIGASPKQRKYYWPVIAAMFAVYW
                     TCSPDEAHETLLIEFAEARREPGQPIKVISTSEMNPSEVEIYYEWCREKGRKHCGMNI
                     PLPRQVLADDEEI"
     gene            complement(3777..4115)
                     /locus_tag="PL000_19145"
     CDS             complement(3777..4115)
                     /locus_tag="PL000_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91057.1"
                     /translation="MKNWVIVSLAGGIMSALAVSLGSFGTYPSDSLAFGMLVLGLSIG
                     ASFAALHYLRSAKLWIAVGGLILGALLCLAFYVYRSINVVYARGIGIVVWTTDPYRQM
                     ILFPGEKCRQ"
     gene            complement(4495..4626)
                     /locus_tag="PL000_19150"
     CDS             complement(4495..4626)
                     /locus_tag="PL000_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91058.1"
                     /translation="MKTMIVDLEFEWTFGIALHVDKVDRIASIFLPFMVVTVVWDGK"
     gene            complement(4623..>5075)
                     /locus_tag="PL000_19155"
     CDS             complement(4623..>5075)
                     /locus_tag="PL000_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91059.1"
                     /translation="PEGSVLLYGETGRFKTMILQYWYNRILAIKMGDPSRMHWIHENQ
                     ITHLAQNDACLSLIDELRSRDVEHFFYDEFLYPINWRSREKGGHYSDMVSRGFMNLFE
                     FFSSNKSTIKVYASGNYKPQDILKDVESEPLLRRQLEVFNSGEGVKEI"
     assembly_gap    5076..5175
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5176..5623)
                     /locus_tag="PL000_19160"
     CDS             complement(<5176..5623)
                     /locus_tag="PL000_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91060.1"
                     /translation="MKAQNKTEAISKWQEAVLALMNANSSGKPKPYKCHDCRDMLWLD
                     VVVTEDGEKITDQNKAWEFAAKTPEAVKHRYCVPCENCASAAVKEAIHERGQFRDGEY
                     VDLKLITKYWGKKKDKDSLVDPIPIAKKLPEGSVLLYGETGRFKTMI"
     gene            complement(5620..6012)
                     /locus_tag="PL000_19165"
     CDS             complement(5620..6012)
                     /locus_tag="PL000_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91061.1"
                     /translation="MNLVFNKRFCSMIARGEKCSTIRKRRPVEIGETLHLFCEQVDSL
                     RKIGKAVIVNFTNISYTPSGVWWLVGQRCFSHDEADAMAVNDGFTDAEELREYLEKIY
                     GRGESDFTIIEWTSFVASEDAKILRGSK"
     gene            complement(6009..6971)
                     /locus_tag="PL000_19170"
     CDS             complement(6009..6971)
                     /locus_tag="PL000_19170"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC91062.1"
                     /translation="MPQQIKTGIAPRLDANFSSAPNRVWDDAEWLPGLHRWVLSHFYR
                     LSLGFRRPSFRISQKKLSRRTGLSVPTLRGILRDLESLGLIARLERWAGSVRLVDEYR
                     LLLVAPERFEGVPEDAEPGDELEADPIPEDGGVGKIFPRGGGKNLSQGGKKTAPIEIQ
                     SLEIQSREVQRQNLSANPSDSLELLPSPAPLPKSSKAKKKPPKEKVVPEGYQKVMDAF
                     HAGLTKLSGDEPVYMNGRDGEGVKKLLRSIPAEKVLEGARILFQLIDSGKNQFYVKRG
                     FRSFVLEQHWNEIMREGKTLRAPGSEKAKSKWREAQEREGFSKI"
BASE COUNT         1717 a         1937 c         1450 g         1890 t
ORIGIN      
        1 taacaatccg tcgatgtcta ttctgcacat ctcccgtcta gttttgccat gcgatccgca
       61 aacttactca atccgtgatc tcgcaaccac tgcgcgcgct tttgcaaatt gtgaattgaa
      121 tcaaactgct tatcgcacca gaaacacagg ctgactatca gctttgaatc agctggatct
      181 tgcacattgc gcgggaataa atgcgaaccg atgattacat gtccgtctat ctttgcgcgt
      241 ccgcagactt tgcatgtata cccatcgcgc acctttgcca tgttcatggc tgaatgaatt
      301 tttgcagatc tggcatgcgc cattttactt tttcatccac agcgcgaatg atgagccagg
      361 gaaatatctt acctcatcct gcggcagcga gcgcacaaga tcccagactg gcttctgcca
      421 ttcccatgct tcctgaaata gaacgcgtcc gcatttcttt gtcaggcccc aatccaacat
      481 agtatcgtca gcatgattcc catcaagata agcaaaatca aaaaccagat gatgattaag
      541 aatctctttt ttttcttcgt tagatcctat taccaaatgc atgaccttgt gcgatacatc
      601 cgcaagagaa aacacttcct gcgcgtatgt gcttttttca acatccattg tgatcacatg
      661 atcaaagtgc cgcgcgagta ccaccgcagt gagaccgtgt cgcgtgccta tttcaaccac
      721 agtgcctcct ttcaccttgc agcgagttag aaattcatcc agcccgtaaa gaacggagga
      781 gcgacgaaac acttcgcccc ccagcctttg ccagattcgc atcagcacgc gatcggaaag
      841 aatcgaggtc atgcgcgagt gcaagagctt ctcaatatgc atttccattc ctggagtaaa
      901 tttcatgtct cgcgctttca cggagcatcg tcctccaaaa agattgcttc gagcaaaatc
      961 aaataattta tcgcatctcc gattttctca tcgatctgag cgcgagatat atcatcgcca
     1021 ttcgcatgat tgcgcacaag atccaaaaca cttacaatgt gcttggtcat aaacccaacg
     1081 caagattccg caggaagctc cctgagaagt tctgctgcgc gtttgaaatt gtgcaaacgg
     1141 tcgtaatccg atgcgtattc cttggacttc agattcgccc tgatcaattc gatgcggcga
     1201 tcaagtatct gtgtaaatcg ttctgggctc atggggctac cttcctttca tcgatctctt
     1261 gctcacgcca ttgaaagcag aaatcatgct ttgatttcgc tactggcttc gggtgcaatt
     1321 tgcaccaaat gtattcttga tgataaacac catcgatcat caaagcatct cctgaatccg
     1381 caacgcgatg aaatccagct ccgcaatttt cgcaactccc tttaattgcg ttcataactt
     1441 atctccgaat tcttttttaa tcgcatcagc caggcgaatc aaggttttct tttctaagcc
     1501 ggaagtatcc aatacgaatt ctgactcaac gtgaatgtcc tttgcagaac taaactgcgc
     1561 gcagaattcc tgcgggagct tctcgacagg ctgtggcatg aagtaacaga caagcgtgct
     1621 aaattgcccc gagtcgtttt cattcttcgg acgcgcaaaa acacaatcct cacagcatcc
     1681 tctgattagc aaaacgcaaa ctcctgcacg gcgctatcga ctggcttttt cagattgaac
     1741 gcatcgcgta acaactcacc gcgaattggc catatcactc cttcaggaac cgcaaacgga
     1801 accaaacgct caagctcacc aggcctgatt gatttctttt tcgcgcgcag ctcgttgaac
     1861 gcattgatgc cgatcgcaaa agtcttattg tacctccgcg atcccaagcc ttgccgcacg
     1921 ttcaaaagaa tcccagatgg cacgccagcg cgcgatgcaa gatccagatt ttccgcctga
     1981 tgatcggaga cattgtcgaa ggaccatgcg tgttcgcaat cgtttgaaaa tttcgcctcg
     2041 gcgaaaaaca caatttcatc gatcgcgaag atgcaatcaa atggtttctg cggaatccac
     2101 cgcatctctc ccattgggaa aatatccggg atcttgtacc agaaagaacg gggatgagac
     2161 aaaactgact tcttccattc ccgcagaaat gcctgctccc ctttccgctt tccgattttc
     2221 cctgctactg ccgatgcagg ggccttgctc attttgaaac cttcgggtct ttcttgcttt
     2281 caaaagtcag ctggccgttt ttctcgacta gcttcttcgc aaggatgaat cgttcggagt
     2341 ccttcatgcc aggaagcgaa atcttcacat cgccctgaat gataatgtca ccagtgcctg
     2401 cctcaggaaa gatagacgct tcaagcatca cttttctgat ctgggtattc tcagaatatt
     2461 ttttgcaatg acgaacggcc tgcgcgatct tcttgtttcc cttctccaga aactcaccgc
     2521 gaagcaccgt ggctaaatca aatttctcca ttttcaacct ttcccatttt gtaagttgag
     2581 gggaccggat gtgatcccct cgtcagtctt ccgactccat ctttttttgc gaatcgcaca
     2641 atccgcgact cgacgctaga gactttttag ctccagcact tcctgcgcga catgaaccag
     2701 cgcgcgattt gtgatcgcgt tctttttcat atacccttca acatccagcg acataagcgc
     2761 ctgtggcaga tctcctgcca ctacgtcgaa agtttcccgc gcttcgaatg atgtgatctc
     2821 ggattttgtc gagtcactca cgcttggcgt ataaactgct ttgaatagcg tgaaccgaaa
     2881 cactttcatg gtaaccacct cctttgtttt ttctttctcg ttttttgttt gaaaaatgag
     2941 accgtgcact gcggcaaatt tcttcaagtc ttccgtatag aagtttgtcc ttgcgcatgc
     3001 cttgcatttg ggatgatgaa taacatactt ctcaactatt ccaccgcgat gatagaacgt
     3061 cccgcgccat gcaccgcaat ccatgcactg tgaaaatttg cattcctcaa gaggcagctg
     3121 aactattccc gcgaccgctc ctgctgtata aaaatctttc tcgcccatgc gctctccctg
     3181 acatagctag attatcctgg caaacatttt tttaaatttc ttcatcatcc gcgagcacct
     3241 gcctgggaag tgggatattc ataccgcaat gcttgcgacc tttctcccga caccattcgt
     3301 aatagatttc aacctcgctt ggattcatct cggaagtaga aatcacttta atcggttgcc
     3361 caggctcacg gcgcgcttct gcaaattcga tcaaaagagt ttcgtgcgct tcatccggtg
     3421 agcatgtcca atagacggca aacatggcag cgatcacggg ccagtagtat tttctttgct
     3481 ttggacttgc accgatggca tctcgctttt tttttctgag aatcaattca tactcaccgt
     3541 cgcgcatctt gcgcacgtat tctgcgaaat catcgcgata ggtgattgtg agctttccgg
     3601 atctgatcga tgcagaccat acgactgaga acttcccgct cataaagggc ctgcaccatt
     3661 cccagcgggc agaaaagcga agcagactgg gaaagaaaat ctggtcgctc tggaaatgat
     3721 gcaggcggag cattcatccg cctgccgggc aattttggat agtcgctttt tttctttcat
     3781 tggcggcact tctcgcccgg aaacagaatc atctgccggt atggatctgt agtccagacg
     3841 actatgccga tcccgcgcgc gtacacaacg ttgatcgagc gataaacgta aaacgcaaga
     3901 cagagaagcg cacctagaat caaaccacca accgcaatcc atagcttcgc ggatcgcaga
     3961 taatgcagcg ccgcgaaaga tgcaccgatg gaaagaccaa gcaccagcat accgaacgcc
     4021 agcgaatcag atggataagt cccgaaactt cctagagata ctgccagcgc gctcatgatg
     4081 ccacccgcaa gtgacacaat cacccaattt ttcatacacc cctcccatgc ggtgaaatca
     4141 gatactttcg atttttccta tgtgtgctac tttcgtattc gatccgcaat gatgcgtcat
     4201 cgtttgtcct gattttcatg tatctgggct ggctaagttt gtttaccatg acgataaaat
     4261 actgtattga tttccggttt gatgaatcgt accgcgaatt tttcagaatc gcgcgatcat
     4321 acctgacgga ccgatctact acgtagtagg cccaatcaaa tttattttgc ggaaacttct
     4381 taaaaagcga ttcgcctttc ttcacaactc cgatcacagt tccagtgcag tagtgatcgc
     4441 cctcagagtt tcccccctga atccatgtga attctttccc gatcagcccg ctcatcactt
     4501 cccatcccaa actacagtaa caaccatgaa cggaagaaag atcgacgcga tgcggtccac
     4561 cttatccacg tgcaatgcga tgccaaaagt ccattcgaat tctagatcca ctatcatcgt
     4621 tttcatatct ccttcactcc ttctcccgaa ttaaaaacct ctaactgcct gcggagcaat
     4681 ggctccgatt ctacatcctt caaaatgtcc tgtggtttgt agttcccact cgcgtagacc
     4741 ttgatcgtgc ttttgttcga cgaaaaaaac tcgaacagat tcataaaccc gcgcgaaacc
     4801 atatcagagt aatggcctcc cttctcgcgt gagcgccagt tgatcggata caaaaactcg
     4861 tcgtaaaaga aatgttccac atcgcgcgat cgcaactcat caatgagcga aagacaagca
     4921 tcgttctgcg cgagatgcgt gatctggttt tcgtgtatcc agtgcatccg cgaaggatct
     4981 cccattttga tggcaagtat gcgattatac caatactgca aaatcatagt tttgaatctt
     5041 ccggtttccc cgtaaagaag cacggagcct tctggnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnaaatc atagttttga atcttccggt ttccccgtaa agaagcacgg
     5221 agccttctgg taatttcttt gcaattggga ttggatcaac aagcgaatcc ttgtccttct
     5281 tcttccccca gtattttgta atgagcttta ggtcaacata ctcaccgtcg cggaattggc
     5341 cgcgttcgtg aatcgcttct ttcactgccg cacttgcgca gttttcacac ggaacacaat
     5401 agcgatgctt cactgcctcc ggagtcttcg ccgcaaactc ccatgcttta ttctgatccg
     5461 tgatcttttc accgtcctcg gtcacaacaa catcaagcca tagcatatct ctgcagtcgt
     5521 gacacttgta cggcttcggc tttccggatg aattcgcatt catgagcgcg agcactgcct
     5581 cctgccattt actaatcgct tcggttttat tttgcgcttt cattttgacc ccctcaagat
     5641 tttcgcatct tcgctcgcga caaatgaagt ccactcgatg atcgtgaaat ctgactcgcc
     5701 acgcccgtag attttctcaa gatattcgcg cagctcctcc gcatcagtga atccatcgtt
     5761 cacggccatc gcgtccgctt catcgtgaga aaagcatctc tggcctacca accaccacac
     5821 gccgcttgga gtgtacgaaa tatttgtgaa gtttacaatc accgccttgc cgatcttccg
     5881 caatgagtct acttgctcgc agaacagatg cagggtttcg ccgatttcga ctggccgccg
     5941 tttccggatg gttgaacatt tttcaccgcg tgcgatcatt gaacagaacc gcttgttgaa
     6001 tacaagattc atattttgct aaacccttcg cgctcttgtg cttcgcgcca tttgcttttt
     6061 gctttctcac ttccgggggc ccggagagtt ttcccttctc gcataatctc gttccagtgt
     6121 tgctctagaa cgaaagaacg aaagcctcgc ttcacgtaga attgattctt tccgctatcg
     6181 atgagctgaa aaagaatcct cgctccttcc agtaccttct cagctggaat acttctcagg
     6241 agttttttca ctccttcgcc atcacgccca ttcatgtaga ctggctcatc cccggagagt
     6301 ttagtcaagc cggcgtggaa cgcatccatg actttttggt atccttccgg aacgactttc
     6361 tccttgggag gtttcttctt ggcctttgac gatttcggca aaggggcagg ggaaggcaaa
     6421 agctcgagcg agtccgaagg attcgcgctg agattttgac gctgtacttc tctagattgt
     6481 atttctaaag attgtatttc tatgggggcg gtttttttcc caccctggga aagatttttc
     6541 ccaccccccc taggaaagat ttttcccacc cccccatctt cagggatcgg atcggcctct
     6601 aattcatcgc ccggctcagc gtcctctggc accccctcaa agcgctcagg agcgacgaga
     6661 agcaggcgat attcatccac cagccgaaca ctaccggccc atcgttccag gcgcgctata
     6721 aggcccagcg actcaagatc ccgaaggatg ccccggagcg tcggaacaga cagcccggtg
     6781 cgccgagaaa gtttcttttg agagatccgg aagctcggcc tgcgaaatcc aagcgacaga
     6841 cgatagaagt ggctcagcac ccagcgatgc agaccaggca accattcagc atcgtcccat
     6901 acgcgattcg gagcgcttga aaaattcgca tcgagtcgtg gagcaattcc tgttttaatt
     6961 tgctgcggca tacccaagca ctccaatcgc gatcatcgcg agaaaacaaa tcaacgtgat
     7021 aaaatctaaa accttctcaa gtgcggacat tgccattcac ctcgtctcca atttcttctt
     7081 cagctccgat agaa
//
LOCUS       DAOQLC010000168         7080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4406_length_7080_cov_22.764413, whole genome shotgun sequence.
ACCESSION   DAOQLC010000168 DAOQLC010000000
VERSION     DAOQLC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_7080_cov_22.764413"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1443)
                     /locus_tag="PL000_19175"
     CDS             complement(1..1443)
                     /locus_tag="PL000_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91063.1"
                     /translation="MKRNILLLAFILIVMAAIVFVLPALVKDVFSNTISAQARRAVIQ
                     TFTIIVVCVSGIVYFSSGAHKREESGMWVTAITLSVFCLTGIGLIWYMVSRSSSNENS
                     PAYYHIITPSSVSGVCEKIDLSALDSQPSKGNPINVRFIDAKADVDCLMNEQSDLASH
                     MKGEAPIYIPTGVFIQSLKFDSANDVILTGFVWQRYANDVPAEIERGFVIPEGDSEIF
                     VREDTAVYKKQQGNETLYGWYINISLRQVFDYSHYPFDRQDIWLRLWHLNFDRNVILV
                     PDFSAYHLEAVWPQDGKTFPGIERDFVLERLSLERTYFSYHYNHYNTNFGIDSYDAHT
                     VFPELYFNVGVRRDVLDAFVAYMIPMFILILMSFGVQFIVTQDPEKMTMHGISTTGLI
                     AYYASLFFIGILAQLDIRRTLNAPGVVYIEYFYFVLYIILLLQTVNSVVFAASDRVEW
                     LEYKDNLIPKVVFFPVTLGLMYLITAFLFY"
     gene            complement(1465..2676)
                     /locus_tag="PL000_19180"
     CDS             complement(1465..2676)
                     /locus_tag="PL000_19180"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC91064.1"
                     /translation="MKWIKSHPWLTGLLVVLAACLVFFFYTDLHLLYRQRPTEFEAAI
                     TQDLADAKIPGAALLFMRDGQVESSKGYGLADVESGRGVDADTLFTVASVSKPVTATA
                     LMTLYEQGKFELDDDINDYLPFEVRNPTFADTPITFRMLLTHTSSIVDSSAYERFYTL
                     KKEPVLPDSPISLEEFVTSYFDPQGEWYDTEENFLKEAPGVAYYYSNMGYGLIGYLVE
                     QISGMPFNEYCKQAIFEPLGMEHSAWFFKDVDVNQVAVPYGYDDLRREPKPFGFYGYP
                     TYPDGALKTSVNEYARFLSVFINEGRTLEGGQFLKPETVAEMLRTQSLLGIEAPAAVG
                     FAWHYDGESYLHTGGDPGISTLVYFNPEADRAVIFFTNGGGEYDVPGVLRLIFVDSKL
                     SPMLLEKMNAP"
     gene            complement(2810..4381)
                     /locus_tag="PL000_19185"
     CDS             complement(2810..4381)
                     /locus_tag="PL000_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91065.1"
                     /translation="MLDTVRPYITKDEPFGPIDSTDIAPGDIKALSLLFDQHNWIYKN
                     LRTRPSIIIGRRGSGKTSYLRSVFFDKQYDHYTEIRTARVLGHMAKVVQKATKEAFFP
                     EMVSELWEMILWVAVFVEVRKHSLLSSDEMYVVNAYLEKLDVRGVDDIDSLFWDLANT
                     LDAALEKNPSRGALDILRGLDRFTFDYAKTIVLESFERSKTSFVVLMDSLDDFQLDID
                     SVAHSLQGLLKLVGSMNKPRDMVDIRFCLPAEVYHRFTAISSNPNKDFRRALRLQWSA
                     SELIFIGARRLGFYLDIFYPDKLRALYPLNVSNRADALRLFQSVLPDRIENQAGFQED
                     TMSYILRHTQLLPRHLLMLLNSIFRKTGVTQTLTPFPVPKERIINGIRQVEELIVKEI
                     FVAFKLIYPTAEETCRRCLPELGHKFTMGDLHRVFTRHGKAVFGGENLFEFQRMLIEI
                     GAIGRVIPGKESDLYIKGNFEYTVQHQLALGQDDEICVHPLFSGIFQNNGKSGRPVYP
                     YGSVLEDADYRDVED"
     gene            complement(4530..5444)
                     /locus_tag="PL000_19190"
     CDS             complement(4530..5444)
                     /locus_tag="PL000_19190"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HNC91066.1"
                     /translation="MESKFIWMNGELVPFEKATLHFLTPALHYGAAVFEGIRAYDTPK
                     GPAVFRLTDHAERLLDSARVFGFRDLPWTTEQIVDAVKQTVKANNFKDCYIRPLLYLD
                     GGGWNLNVDYGKASLAIAAWEWGNYLGEEARAKGIRANISSFTRHHPNVLMTKAKVAG
                     NYANSFLAKTESERLGFQEAIMLDPQGYVAECTGENLFMVRHGKIVTPSTAPVLEGVT
                     RHSIHTIAKDMGFRILEQPISRDQLYIADEVFVCGTAAECIGLSEIDFRKIGDGMTGP
                     VTKRIQDTYHEAIRGKLPQYEAWCEYVD"
     gene            complement(5519..6577)
                     /gene="leuB"
                     /locus_tag="PL000_19195"
     CDS             complement(5519..6577)
                     /gene="leuB"
                     /locus_tag="PL000_19195"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNC91067.1"
                     /translation="MNFKITLLPGDGIGPEVVGEAVRVLETIASKYKHSFQFQERLMG
                     GCSIDKYGSSLTDETLADCQASDSVLLGAVGGPKWDDPKAKDRPERGLLALRKGLGVF
                     ANLRPVKVHPALMEWSPLKPEKLQGVDILVVRELTGGLYFGFPKGRDIKDGRERAVDT
                     LEYYDYEVKRVMELAFNLARGRKKKVTSVDKANVLESSRLWRQIAVKVGEANKDIELE
                     HTLVDTASMRLITGPAWMDVVVTENMFGDILTDEASVLAGSMGMLPSASLGESTVGLY
                     EPIHGSAPDIAGKGIANPIGTILSTAMMLRHSFKLESEAAAIEKAVDETVTAGARTAD
                     IGGKLTTRQMADEIINRL"
     gene            complement(6634..>7080)
                     /locus_tag="PL000_19200"
     CDS             complement(6634..>7080)
                     /locus_tag="PL000_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNC91068.1"
                     /translation="FEAAEASVAIMLKRQEYGYKPPFELIDFFVNVEHRQGRGIFAEA
                     MVKVRVQGEVLHTAAEGNGPVNALDLALRKALKDYYPQIMKFNLSDYKVRILDSDHGT
                     EAITRVLIDTRNSNSRWSTVGAGTNIIEASWRALADSVEYGLMVAH"
BASE COUNT         1845 a         1811 c         1757 g         1667 t
ORIGIN      
        1 ctagtaaaac agaaaggctg tgattaaata catcagtccg agagtaacag ggaaaaaaac
       61 aactttcggg atcaggttat ctttgtactc cagccattca acacgatcag aagcggcaaa
      121 gacaaccgag ttgaccgttt gtaaaagcag aatgatataa agaacaaaat aaaaatattc
      181 aatatacacg acaccgggcg cattcaatgt gcggcggata tcaagttgag ccagaatgcc
      241 gatgaaaaag agcgaagcat agtaagcgat caacccggtg gtgctgatgc catgcatggt
      301 catcttttcg gggtcttgcg taacgataaa ctgtacgcca aaagacatca aaatcaaaat
      361 aaacatgggg atcatgtatg ccacaaatgc gtccaaaaca tcgcggcgca cacctacgtt
      421 gaaatacaat tcaggaaaaa cggtgtgggc atcgtaactg tcaatgccaa aattggtgtt
      481 gtaatgattg tagtgataac taaagtaggt tcgctccaac gagaggcgtt ccagcacaaa
      541 gtcacgttcg atgccgggga aggtctttcc atcctgcggc cagaccgctt cgaggtggta
      601 ggcggaaaaa tcagggacca gaatcacatt tcggtcaaaa ttgagatgcc acaggcgcag
      661 ccagatatcc tgcctgtcaa atggataatg ggaataatca aacacctgac gcaaggaaat
      721 attgatatac cagccataca gggtttcatt tccctgttgt tttttgtata ccgctgtatc
      781 ttcccttaca aagatctcag aatcaccttc gggtatcaca aaaccacgtt caatctctgc
      841 aggaacatca ttcgcataac gctgccagac aaatcctgtc aaaatcacat cattggcgct
      901 gtcaaatttc agtgattgga taaacacccc ggtggggatg tatatcggcg cctccccctt
      961 catatgactg gcaaggtcag attgctcatt catcaagcaa tccacgtcag ctttggcatc
     1021 aatgaaacgg acgttgatcg ggttaccctt gctcggctga gaatcgagcg cgctgagatc
     1081 aattttctcg caaacgccgc tcacgctgga cggcgtgatg atatggtaat acgcagggct
     1141 attctcattg gacgaacttc ttgagaccat gtaccagatc aggccgatac cggtcagaca
     1201 gaaaacagaa agagtgatcg ccgtcaccca cattccgctt tcttcacgtt tgtgagcacc
     1261 actggagaaa taaacaatgc ccgaaacaca gacgacaatg atcgtaaacg tttgaatcac
     1321 cgcccgccgt gcctgcgcag aaatggtgtt cgaaaagaca tctttcacaa gggcaggcag
     1381 gacaaataca atcgccgcca tcacaataag aataaaggcc agaagcagta tgttacgttt
     1441 catgagcacc tcaacacatc aagcttatgg agcattcatt ttttccagca acatggggga
     1501 aagttttgaa tctacaaata taaggcggag cacaccgggc acatcatatt caccgccgcc
     1561 attggtaaag aagatgaccg cacggtcagc ttcggggttg aaatacacca atgtgctgat
     1621 gcccgggtca ccgccggtat gaagatagga ttcgccgtca taatgccagg caaaccccac
     1681 agcggcaggc gcttcaatac ccagcaggct ttgcgttctg agcatttctg caaccgtttc
     1741 aggttttaga aattgtccgc cttcaagtgt tctgccttcg ttgataaaca cagaaaggaa
     1801 acgggcgtac tcattcacgc tggtcttcaa tgcaccatca ggataggtgg gatacccata
     1861 aaagccaaag ggttttggtt cgcgcctcaa atcgtcataa ccgtatggaa ccgccacctg
     1921 atttacgtcc acatccttga agaaccaggc agaatgttcc atgccgagcg gctcaaaaat
     1981 cgcctgtttg caatattcat tgaagggcat gccgctgatc tgctccacaa gataaccgat
     2041 caagccatag cccatgttgc tgtaataata ggcaacgccg ggcgcttcct ttaggaagtt
     2101 ttcctcggtg tcgtaccatt caccctgcgg atcaaaataa gaggtgacaa attcctcaag
     2161 cgaaatgggc gaatccggta aaaccggttc cttcttcaag gtatagaaac gttcataggc
     2221 actgctatcc acaatgctgg acgtgtgggt aagcagcata cggaaagtga tgggggtatc
     2281 ggcaaaggtg gggttgcgta cttcgaacgg cagataatcg ttgatgtcat catccaactc
     2341 gaatttaccc tgttcataaa gcgtcatcaa cgctgtggca gtgaccggct tggagacaga
     2401 tgcgaccgta aaaagcgtat cagcatccac gccccggcct gattcaacat ctgcgagacc
     2461 gtagcccttt gaagattcca cttgtccatc gcgcatgaaa agtaacgcgg cgccggggat
     2521 cttcgcatcg gccaggtctt gagtgatggc ggcttcaaac tccgtggggc gttgacggta
     2581 gagaaggtgc aggtcggtat agaagaagaa aacgagacag gccgccagca caaccagcag
     2641 acctgtcagc caggggtgag atttgatcca tttcatgtga aaatcctttt tgcaagttca
     2701 ttgtccacgc agcggaaagg gttcggctgc ggaaatttgt cagaaaacag aagcagcaaa
     2761 aagcgcgtcg cctggacgcg cttttttagt cgcagtccaa cttcaagggt cagtcttcca
     2821 catctcgata atctgcatct tcgagcacac ttccataagg atagaccgga cggcccgatt
     2881 taccgttatt ttgaaaaatt cccgagaaga gcgggtggac acaaatttca tcatcctgac
     2941 ccaaagcgag ttgatgctga acggtatatt caaaattgcc tttgatatac aggtcagact
     3001 cttttccagg aatcaccctg ccaatggcgc cgatttcgat cagcattctt tggaattcaa
     3061 aaagattctc gccgccaaag accgctttgc catgtcgtgt gaaaacgcgg tgcaggtcgc
     3121 ccatggtgaa cttgtgtccc aactcgggca ggcagcggcg gcaggtttcc tcggcagttg
     3181 ggtagatgag tttgaaagcc acaaaaattt ccttgacgat caactcctcc acctgacgaa
     3241 tgccattgat gatgcgttcc ttgggaacgg ggaatggtgt gagggtttgt gtaacccctg
     3301 tttttcggaa gatggaattc aacaacatca acagatggcg cggcaataat tgcgtatgcc
     3361 tgaggatgta agacatcgtg tcttcctgaa acccagcctg attctcgatc cgatctggca
     3421 gcaccgattg gaacaaacgc agggcatcgg cacggttgga aacgttcaac gggtaaagcg
     3481 cgcgcaattt atcggggtag aaaatatcaa ggtaaaaacc aagcctgcgc gcgccaatga
     3541 agatcaattc gctggccgac cattgcaggc gcagggcgcg gcggaagtct ttgttggggt
     3601 tggaggagat cgctgtaaaa cggtgataca cctccgcagg caaacagaag cggatatcca
     3661 ccatatcgcg cggcttgttc atggagccga ccagtttcag aaggccctga agcgaatgcg
     3721 ccactgagtc aatatccagt tggaagtcgt ccagcgaatc catcaacacg acaaaggagg
     3781 tctttgaacg ttcgaaactt tccaaaacaa tggttttggc gtaatcaaag gtaaagcggt
     3841 ccagtccgcg caaaatatcg agcgcgccgc ggctgggatt tttttccaga gctgcatcga
     3901 gggtgttggc aaggtcccag aacaggctgt caatgtcatc cactccgcgg acatccaatt
     3961 tttcgagata ggcattgacc acgtacattt catcggaaga aagcagggaa tgtttccgca
     4021 cttccacgaa gacggccacc cacaggatca tctcccaaag ttcggagacc atctccggga
     4081 aaaaggcttc tttcgttgct ttctgtacca ccttggccat gtgccccaaa acccttgcag
     4141 tacggatctc agtgtaatgg tcatattgtt tatcgaaaaa gacactgcgc aagtaggagg
     4201 ttttccccga acccctgcgg ccaatgataa tggacggcct ggtacgtagg ttcttgtaga
     4261 tccagttatg ctggtcgaag agcaggctta atgccttgat atcgcccggg gcaatgtcag
     4321 ttgagtcgat cggtccaaat ggctcgtctt ttgtaatgta ggggcggaca gtatccaaca
     4381 tggctgggat ttcctccttg tggggtgaaa actctgggcg gattatacaa tcaaacccga
     4441 aattgcgatg aacagaggcg gatagccaga aagcaaaaag aaaatcgggc gaagcttttt
     4501 gcttcacccg attttgatct ctgtcacacc tagtccacgt attcgcacca cgcttcgtat
     4561 tgtgggagtt ttccgcgaat cgcttcatga taggtatctt gaatgcgttt ggtcacaggc
     4621 ccggtcatgc cgtcaccgat cttgcggaaa tcaatttcac tcaagccaat gcattcagcc
     4681 gccgttccac acacaaagac ctcatcggca atgtaaagct ggtcacgtga gatcggctgt
     4741 tccagaatgc ggaaacccat atccttggca atggtgtgga tcgaatggcg ggtcacacct
     4801 tccaacacgg gcgcggtgga cggggtgacg atcttgccgt ggcgaaccat gaacaaattc
     4861 tcacctgtac attccgccac atagccttgc ggatcgagca tgatggcttc ctggaaaccc
     4921 aatcgctctg attcggtttt ggcaaggaaa ctgttcgcgt aatttccagc cactttggcc
     4981 ttggtcatca acacattcgg gtgatgacgc gtgaacgaag aaatgttcgc gcgaattccc
     5041 tttgcgcgtg cttcttcacc gaggtagttg ccccattccc atgcggcaat ggcaagagaa
     5101 gccttgccgt aatccacatt cagattccag ccgccgccat ccagatacaa gagcgggcga
     5161 atgtaacaat ccttgaaatt attggccttc accgtttgct tcaccgcatc cacgatctgc
     5221 tcggtcgtcc acggcaggtc acggaacccg aacacacgcg ccgaatcgag caggcgttct
     5281 gcgtggtcgg tcaagcgaaa gaccgcaggt cccttcgggg tgtcatacgc gcgaatccct
     5341 tcgaacaccg ccgcgccata atgcaaagcg ggggtgagaa aatgcagagt cgctttttcg
     5401 aacggcacaa gctccccgtt catccagata aatttggatt ccattcccat gattcaattc
     5461 tccttttttt gttcgaccac agaccgcaga cgacagattg tcatccatga tccatcgtct
     5521 atagtctgtt tatgatttca tccgccattt gacgggtcgt taacttaccg ccgatatctg
     5581 ccgtgcgcgc ccctgcagtc acagtttcat ccactgcctt ttcaatggcc gcggcttcag
     5641 attctaactt aaacgaatga cgcagcatca tcgccgtgga aaggatcgta ccaatcggat
     5701 ttgcgattcc tttgcctgca atatctggag cagacccatg gatcggttca tacaacccga
     5761 cggttgattc gcccaaactg gcagaaggca acattcccat tgatcctgcc aaaaccgaag
     5821 cctcgtcggt gaggatatcg ccgaacatat tctcggtgac gacaacatcc atccatgcag
     5881 gaccagtgat caaacgcatg gatgcagtat ccaccagagt gtgttccaac tcaatgtcct
     5941 tgttcgcctc acccactttg acagcgatct gccgccacaa acgtgaggat tcgagcacat
     6001 tggctttatc caccgaggtg acctttttct tgcgtccacg ggcgaggttg aaggcaagtt
     6061 ccataacacg ttttacttcg taatcgtaat attccagtgt atccaccgca cgttcgcgtc
     6121 cgtctttgat atcgcgtccc ttggggaagc caaagtacag accgcccgtg agctcacgca
     6181 cgacgaggat atccacgcct tgcagtttct caggcttgag cggtgaccat tccatcaacg
     6241 ccgggtggac tttgaccggg cgcaggttgg caaatacgcc gagacctttg cgcagggcga
     6301 gcaatccgcg ctcggggcgg tctttggctt tggggtcgtc ccatttcggt ccgccgacag
     6361 cacccagcaa aacggagtct gaggcctggc aatcggcgag agtctcatcg gtcagggaag
     6421 agccgtattt gtctatcgag cagccgccca tcaatcgttc ctggaattga aatgagtgct
     6481 tatatttgct ggcaattgtt tccaacacgc gtacggcttc gccgaccact tctggtccga
     6541 tgccatcgcc ggggagtaag gtgattttga agttcattgt tctccgtaat ctctaattac
     6601 caattacttt ttaccgtaat tggcaattgg taattaatgc gccaccatca atccatattc
     6661 cactgagtct gctaatgcac gccatgaggc ttcgataata tttgtgcctg cgccaacggt
     6721 gctccagcgg gagttggaat tgcgggtgtc gatcagcact cgggtaatgg cttcggtgcc
     6781 gtggtccgaa tccaaaatgc ggactttgta atcggacaaa ttgaatttca tgatctgcgg
     6841 gtaatagtct ttgagcgcct tgcgcaaggc gaggtcaagc gcgttgacgg ggccattgcc
     6901 ttccgcagcg gtatgcaaca cttcgccctg cacgcggacc ttgaccattg cttcagcaaa
     6961 aataccgcgt ccttgtctgt gttcgacgtt gacaaagaaa tcaatcagtt cgaagggcgg
     7021 tttgtagccg tattcctgcc gcttgagcat gatcgccaca gaggcttcgg cagcttcaaa
//
LOCUS       DAOQLC010000169         7049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4438_length_7049_cov_15.403060, whole genome shotgun sequence.
ACCESSION   DAOQLC010000169 DAOQLC010000000
VERSION     DAOQLC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4438_length_7049_cov_15.403060"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..2657)
                     /locus_tag="PL000_19205"
     CDS             complement(114..2657)
                     /locus_tag="PL000_19205"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC91069.1"
                     /translation="MAAQQKQTSSPQNQFFTNTFGDALKYLRKRAHLTQDELGRAVGY
                     SREQIARLENGSRVPDLAVVAALFVPALDIQHQPELIQRLLELAGQIRSAPGESQHIT
                     VAHTTQTKTQRIVEVVSQSAPLPYTLPSPLFPLIGRVSEVESLCALLLGQARLITLIG
                     TAGIGKTRLALETAHALTGNFSHGACFVELAPIQNAEAVPGAIASALGITPTADQPIE
                     TAVAAHLAPHETLLVLDNCEHVLDAAVYFDKLLAVAPKLKLLCTSRTALDLYGEHEWE
                     VSPLSLPILSQLPDPDQLSQIAAVQLFLARIRTFDSAFRLDSSNAVSIATLCVALDGL
                     PLALELAAARVREIKPADLLHQIVSTRSRPQLSSTLLQQTKRNISARHRTLHEAIAWS
                     YRLLSPAQQAAFIRLGVIVGGCTVEAAQNICDTDLPTLQALASASLIQLESNPSPGRV
                     NMLETLRSFALEQLIHANLLNTLKRKHAAHFAEYAGVIFKEIRGGQQSQWLAQTRLEH
                     DNFRSALLFAVEEQDSHLAVSISGGLWWFWYRQGFLSEGREWLDKSLRIPMKHQPSET
                     QKRARATALNGAGSMACEQCDYPSAMAYHKEGLALRYELEDGEGIAAVLHNMALVARS
                     QGDYPRAIELFEKSLTMEKETGSDSSLAMSYANIGITAGEMNDLPLAKSWLNRALEKV
                     NVDELPWETAFIALNLAQILYWQEDFTQAEALARQSLRSFEEQGDQLYGPEAQLILAQ
                     TAIEHGDFSLAQSLCGNVLAYYQGLNDEHGISNVLSVLAWITLHEDHNNEGAATAARL
                     FSESMFLREKNKRALSPMEKIRNQMLEDAIHRLASQQPGSREAADKKEP"
     gene            2836..3018
                     /locus_tag="PL000_19210"
     CDS             2836..3018
                     /locus_tag="PL000_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91070.1"
                     /translation="MDRRLLVIRMTEVVEVTETITIHVKNDALKDSETDVVVEETELR
                     WVQLEFTDLLIKAPEK"
     gene            3038..5191
                     /locus_tag="PL000_19215"
     CDS             3038..5191
                     /locus_tag="PL000_19215"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91071.1"
                     /translation="MKKRIALLVLILNLVAAALQVQPARAANAVVGTGTPASCTETAF
                     DNALTTAGSGGGTITFNCGSGTHTITFSTSKTVNLGNVTIDGGNSIILAGSSTVRQFF
                     VGGGVTFTLKNITLRDGDSLVGGGAIEASTATVILDSVQILNNYSAVQGGAIYCYDGT
                     VSASDTLFSGNESSTGGAIFNDGCNITVNGSTFQNNKTSTSGGAIYNSMLGVLQVSSS
                     QFTGNQSLDGGGIFNASGATATLNAVTMSSNSGGHGGGVENSGTMTINDSIIDSNSVT
                     GSGGGIWNMGGALTLARTSVNNNRAFEGAGINSYGTQVQITNANITNNVTTGTHGGGI
                     FINAGTASITNATISGNQAVGAAADGGGIYHNSNDNLTLTNVTLVSNQAGARGGGLYH
                     YGNYAILTNVTIGNNTAVMAGPAIYEDSPNTAVVQMKNSVVFGSAGNCDGGVFQSLGY
                     NISKGSCPSLNQATDQDNFAGDIAMGALAFNGGIFPMQTMMPLSGSPVIDAGDACSAT
                     DQLGAARPVGAGCDIGAVEFGASTSTFADVPSSYWAWQFIERLYAAGITGGCSTSPLN
                     YCPESGVTRAQMAIFLLKGIHGSTYTPPAVGASTGFNDVSTSYWAAAWIKQLAAEGIT
                     SGCGGGNYCPDATVTRAQMAIFLLRAEHGSAYMPPAASGIFTDVPVGYWADAWIEQLA
                     AEGITSGCGTGIYCPEADVTRAQMAVFLVRTFKLP"
     gene            5275..>7049
                     /locus_tag="PL000_19220"
     CDS             5275..>7049
                     /locus_tag="PL000_19220"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNC91072.1"
                     /translation="MKIQILVSLLVILACIMIPSQVPPAAALGNVIYVSTAGSNSTGD
                     GSISNPFQTIGHAITTATAGDEIVLRGSPAVADNHYEESIRIEIPNITVRSQDGEWAV
                     IECPFNNENITECVRLDVDSSGSKIRSLEIIGGYYYGIKLETRWDWGDPSDRSGASNI
                     LIEGVVIHNTGRDAIKITPGCDDVTIRRVEIYNTGVRDNSNAEGIDNVNGDRMIVQDS
                     YLHDIATTGIYFKGGAMDGIVERNRIERTGEAGILIGFDTSPEYFDLTVNPSYYENIR
                     GIVRNNIISDIPYSGIGLYAAQNAQVLNNTIINTAQVGHSPIYFGITYQDWDPAATRP
                     SSVNPIIQNNLISQPAGQSGECVFIRYSEDLGGLSALSGMPMMDYNILYHAGSNCLFT
                     DRRPGSILETGTFSQWQAHINGDVHSLAMDSQVLTDGSLPSGSPAIDSGSNSNCPSTD
                     QRGAGRPHDGNGDATAICDIGAYEFGAPTNTTFTDVPLTHPLYKYIEALYDAGYTAGC
                     SSSPMMFCPDTILDRAQSAVFMLRGQKGSGYTPPAVTGIFGDNWTGFEWAQGWAEGMY
                     QEGLTTGCQSSPLLFCPANQLPRVEA"
BASE COUNT         1677 a         1745 c         1918 g         1709 t
ORIGIN      
        1 aattggtgaa tcgcggctgg ggtgcgtacc tgatcgtcaa agccaagaat ttgccgacgc
       61 cgtaaaataa gatacgcgtt acatcccata aaaagagccg tgagatcaaa atctcacggc
      121 tcttttttgt ctgcagcttc cctgcttcca ggttgctgac ttgccaatcg atgaattgca
      181 tcctcaagca tttgatttct gattttttcc ataggtgaca aggcacgctt atttttttcg
      241 cgtaaaaaca tagactcgga aaacagtctg gcggctgttg ccgcaccctc gttgttatga
      301 tcttcgtgaa gggtaatcca tgccagaacg gaaagtacat ttgagattcc atgctcatca
      361 ttgagcccct gataatatgc cagaacattg ccgcacagag attgagccag cgaaaaatcg
      421 ccgtgttcaa tggcggtctg tgccaaaatc agttgtgctt caggcccata aagttgatca
      481 ccctgctctt cgaacgaccg cagactctga cgggccaatg cctccgcctg tgtaaaatct
      541 tcctgccagt acaaaatttg ggcgagattt agtgcaataa aagccgtttc ccaagggagt
      601 tcatcaacat tcactttttc aagcgcgcgg tttaaccagc tttttgccag tggaagatcg
      661 ttcatttcgc cagcggtgat accgatgttg gcataactca ttgccagaga actgtcactg
      721 ccagtctcct tttccatggt aagagatttt tcaaatagtt caatggcgcg tggataatct
      781 ccctggctgc gggcgaccag agccatattg tgcaatacag cggcgattcc ctctccatct
      841 tccaattcat accgtaatgc caacccttct ttgtgatacg ccatggcaga aggataatcg
      901 cattgttcac atgccatgga ccctgctcca ttgagcgctg tagcacgtgc ccgtttttgg
      961 gtttcgctgg gttgatgttt cattggaatt cgtaaacttt tatcgagcca ctccctgcct
     1021 tcactcaaga atccctgtcg ataccagaac caccataacc cgccagaaat ggaaaccgcc
     1081 agatgcgagt cctgctcttc cacagcaaag agcagcgcag acctgaagtt atcgtgttcg
     1141 agtctcgttt gggccagcca ctgagattgc tgtccaccac ggatctcttt gaatatgacc
     1201 ccggcatatt ctgcaaaatg ggcggcgtgt tttcttttca aagtattcaa cagattggcg
     1261 tggatcagtt gttccaatgc aaaggatcgc aaagtttcaa gcatattcac ccgccccggg
     1321 gacggattgc tttcaagttg tatcaggctg gccgatgcca aagcctgcaa agttggtaga
     1381 tctgtgtcgc atatattttg ggcggcctcc actgtgcagc cgccaacaat gactcccagc
     1441 cgtataaagg ctgcctgttg agcaggggaa agcagccggt aactccatgc aatggcttca
     1501 tgcagggttc ggtgcctcgc tgaaatattg cgcttggtct gttgcagcaa ggtggaggat
     1561 agttgtggac ggctgcgggt gctgacgatc tggtgcaaca ggtcagcagg tttgatttca
     1621 cgcacccggg ccgcggctaa ttccagtgca agaggcaatc catccagtgc aacgcataaa
     1681 gtggcgatgg aaacggcgtt gcttgaatcc aacctgaaag cggaatcgaa ggtccggatt
     1741 cgcgccaaaa ataactggac tgctgcgatc tgactcagct ggtcagggtc aggcaactgt
     1801 gacaggatcg gcagggatag tggggagact tcccattcgt gttcgccgta caggtcaagt
     1861 gcggtgcggc tggtgcagag gagtttgagt ttgggggcaa cggcgagcaa tttatcaaaa
     1921 tacacagccg catcaaggac atgttcgcag ttatccaata caagcagggt ttcatgaggc
     1981 gcaaggtggg cggcaactgc ggtttcaatg ggttggtctg ctgtgggggt gatgcccaac
     2041 gccgaggcaa ttgcaccggg gacggcttct gcgttttgga tgggagccag ttctacaaaa
     2101 catgcgccat gtgaaaaatt accagtcagt gcatgggctg tttcaagggc cagccttgtt
     2161 tttccgatac ctgctgtgcc gatcagggtg atcagccgcg cttggccgag taaaagcgcg
     2221 caaagagatt cgacttccga aacgcggcca atcagcggaa agagcggaga gggtagtgtg
     2281 tagggtaatg gcgcagattg gctaacgact tcaacgattc tttgggtttt ggtttgggtg
     2341 gtatgggcaa cggtgatgtg ttgagactcg ccaggggcgc tgcggatctg cccggctaat
     2401 tcgaggaggc gttgaatgag ttcaggttga tgctgaatat ccaatgctgg gacgaagagg
     2461 gcggcaacca cggcaaggtc tggcacacgg cttccatttt caaggcgggc gatctgttcg
     2521 cggctatagc cgacagcgcg ccccaattca tcttgtgtca ggtgggcgcg ttttcgtaaa
     2581 tatttgagcg catcgccaaa tgtgtttgta aaaaactgat tctgtgggga agaggtttgc
     2641 ttttgttggg cggccatggg tgaatcatag catattcaat ggagtggggg cattttttag
     2701 gagggatggg tcaacggcaa ttgaggccgt tcggacggta tcaaatctcg gttatgtggg
     2761 agtcgtcttt tgtccatatt tttgcctgtc acaaaatgtc acctttggtc acgacccaac
     2821 ctgtatgatg cggacatgga tcgccgtttg cttgtcatca gaatgaccga agtcgtagag
     2881 gtgaccgaga cgatcacgat tcacgtcaaa aacgatgcct tgaaagattc agagacggat
     2941 gtagttgtgg aagagacgga acttcgatgg gttcagcttg aatttaccga tctgctgata
     3001 aaggcgccag aaaaataata tcaacaagga gatgaccatg aaaaaaagaa ttgctttgct
     3061 ggttttgatt ttgaatttgg tggctgcggc tttgcaggtg cagcctgccc gcgctgccaa
     3121 tgcggtggtt gggacgggaa cacccgcttc ctgtacagag accgcattcg ataacgcatt
     3181 gacgacggct gggagtggcg gtggtacgat cacgttcaat tgcggttcag gtacacacac
     3241 gatcactttt agtacttcca agacggtcaa tttgggaaat gtaaccattg acggcgggaa
     3301 ttcgatcatc cttgcgggtt cgtcaaccgt gcgtcagttt tttgtcggcg gcggagtaac
     3361 gtttaccctg aaaaacatca ctttgcgcga tggtgattcg ttggtgggtg gcggcgcaat
     3421 tgaggcatcc acggcaacag tgattttgga ttcggtgcag atcctgaata attattcagc
     3481 ggttcagggc ggtgcgattt attgttacga cggcaccgtg tcggccagcg acaccctgtt
     3541 cagtgggaat gaatcgagta cgggaggcgc gatcttcaat gatggttgca acatcacggt
     3601 caatggttcg accttccaaa acaataagac gtctaccagc ggcggcgcga tctataacag
     3661 tatgcttggt gtgttgcagg tcagcagcag tcaattcact gggaatcagt ctcttgacgg
     3721 cggcggtatt ttcaatgcaa gcggcgccac ggctaccctt aatgcagtga caatgagttc
     3781 caattcaggc gggcatggcg gtggcgtgga aaacagcggc accatgacca tcaatgacag
     3841 catcattgac tcgaattctg ttactgggtc tggcggcggc atctggaata tgggcggcgc
     3901 gcttacgctg gcacgtactt cagtgaataa caacagagcc tttgaaggtg cagggatcaa
     3961 cagctacggc acacaggtac aaatcaccaa tgccaatatc acaaacaatg tcaccacggg
     4021 tacgcatggc gggggtattt tcatcaatgc tggcacggct tccatcacca atgccaccat
     4081 cagcggaaat caggctgttg gagcggctgc cgatggaggc ggtatctatc acaattcgaa
     4141 cgataatctg acgttgacca atgtgacgct ggtttccaat caggcgggcg cacgcggggg
     4201 cgggttgtat cactatggta attatgccat tttgaccaac gtgacgattg gtaataatac
     4261 tgctgtcatg gctgggcctg cgatctacga agattcgccg aacaccgctg tggtccaaat
     4321 gaagaacagt gtggtctttg gcagtgctgg caattgtgac gggggggtgt tccaatcact
     4381 tggctacaac atcagcaagg gatcatgccc gtctttgaat caggccaccg atcaggataa
     4441 ttttgcaggt gatattgcca tgggagcgct tgccttcaat ggaggcatat tccccatgca
     4501 gaccatgatg ccgctttcag gcagcccggt gattgatgca ggagatgcct gctctgccac
     4561 cgatcagctt ggcgcagcgc ggcctgtggg tgcaggttgt gatattggtg ccgtggaatt
     4621 tggtgcatcc acttccactt tcgccgatgt gcccagttcc tattgggcct ggcaattcat
     4681 cgaacgttta tacgcggctg gcatcaccgg tggttgttcc accagcccat taaattactg
     4741 ccccgaatct ggtgtcaccc gtgcgcaaat ggccatcttc cttcttaagg gaatccatgg
     4801 ctcaacctac actccgcctg ctgtgggtgc aagcactggc ttcaatgatg tttccacaag
     4861 ttattgggcc gcagcatgga tcaaacaact ggctgctgaa ggcatcacca gcggttgcgg
     4921 cggcggaaat tattgccccg atgccacggt cacccgcgcc caaatggcca tcttccttct
     4981 gagagccgag catggatccg cctatatgcc gcctgccgcc agtgggattt tcacagatgt
     5041 ccccgtcggc tattgggcag atgcctggat cgaacagttg gccgccgaag gcatcaccag
     5101 cggatgtggt acaggaattt attgccccga agcagatgtg acccgcgccc aaatggcggt
     5161 ttttctagtg cggaccttca aactgccgta acgaatcgag cgccccgcaa actgattcaa
     5221 cccaatttgc ggggcgctcc ctttgggcct gcttttgcga aaggaaaaat aaaaatgaag
     5281 atccagatat tggtcagcct gcttgtcatt ctagcctgca tcatgatccc atctcaagtt
     5341 ccacctgccg cagcactggg aaatgtgatc tatgtctcaa cagcggggag caacagtaca
     5401 ggggatggca gcatctccaa tccgtttcaa accattggcc atgccattac aaccgccacc
     5461 gccggagacg agatcgtttt gcgcggctct cctgcagttg ccgacaatca ctacgaagaa
     5521 agcatccgca tcgaaattcc gaacatcacc gtccgctcgc aggatggcga atgggcagtg
     5581 atcgaatgcc cgttcaacaa tgaaaatatc accgaatgtg tccgccttga tgtggatagt
     5641 tccggcagca agattcgcag cctcgaaatc atcggcggat attattatgg tatcaaactt
     5701 gaaacccgtt gggattgggg agatccctca gatcgcagtg gagcctccaa tattttgatc
     5761 gaaggtgtgg tgatccacaa tacagggcgc gatgcgatca agatcactcc tggctgtgac
     5821 gatgtaacca tccgtcgtgt tgaaatttat aacaccggtg tgcgcgataa ctcgaacgcc
     5881 gaagggattg ataacgtgaa cggtgaccgt atgatcgttc aagacagcta cctgcacgat
     5941 attgccacga ccggtatcta tttcaaaggc ggggctatgg atggcattgt tgagcggaat
     6001 cgcattgagc gcacagggga agcggggatt ttgattggct tcgataccag ccccgaatat
     6061 ttcgacctta cggtaaatcc cagttactat gagaacatcc gcggcattgt gcggaacaat
     6121 attatcagcg atatacctta ctcgggtatt ggcttatatg ccgcacaaaa tgcgcaagtc
     6181 ttgaataaca ccatcatcaa cactgcacag gttgggcata gccccatcta ctttggtatt
     6241 acataccaag attgggaccc cgctgccaca cgaccttcat ctgtgaatcc gatcatccaa
     6301 aataatctca tttcacagcc agcagggcag agcggcgaat gtgtttttat tcgctactca
     6361 gaagatctgg gcgggctttc tgccttaagc ggaatgccaa tgatggatta caacattctg
     6421 tatcatgcag gcagcaactg cctgtttacc gaccgccgcc ccggcagtat tttagagact
     6481 gggactttct cacagtggca ggcacacatc aatggagatg tgcatagtct cgcaatggac
     6541 tcccaggtgc tgacagatgg aagcctgcct tcaggcagcc cagccattga tagtggaagc
     6601 aactcgaact gcccaagcac cgatcaacgc ggtgcggggc gtccgcatga tggaaatggt
     6661 gacgcaactg ccatttgcga tatcggtgcc tacgaatttg gtgcgccaac caacaccact
     6721 tttaccgatg tgccacttac gcacccgttg tataaatata tcgaagccct ctacgatgca
     6781 ggctacaccg caggctgctc cagcagcccg atgatgttct gccccgatac gattctggat
     6841 cgtgctcaat ccgccgtctt catgttgcgt ggacagaagg ggagcgggta caccccacct
     6901 gccgtcacgg gcatcttcgg cgataactgg acaggtttcg aatgggcgca aggttgggca
     6961 gaagggatgt atcaggaagg cctgaccaca ggctgccaga gcagtccctt gctgttttgt
     7021 ccagcgaatc aactgccgcg tgtggaggc
//
LOCUS       DAOQLC010000170         7043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4442_length_7043_cov_20.285203, whole genome shotgun sequence.
ACCESSION   DAOQLC010000170 DAOQLC010000000
VERSION     DAOQLC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4442_length_7043_cov_20.285203"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="PL000_19225"
     CDS             <1..1547
                     /locus_tag="PL000_19225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91073.1"
                     /translation="VASYGFYYADVPIFAEMNTLLRKNLLRYILKRPGASQLPDSPGE
                     AVSRFKKDVDEIPLFVILINDIMVGLIIIAVAIILMTQISPSVTVMALIPLALVGIVA
                     NLATGRIEHYRRSSRQAGGKVTGFIGEFFGAVQAVKVATAEKDVIHHFHKLNDERRVL
                     TIREKLFDEVLGSIYRNMSTLGTGVILILVGQSMREGNFTLGDFSLFVYLLQSMGDLT
                     TFGGMLAARYKQLDVSVQRMYHLMENAPLNALVEHSKIDLDGPLPEVIYPTKTASDSL
                     RSLSADHLTFHYPDSVNGIEDISLKINRGSLTVITGRIGSGKTTLLRALLGLLPIESG
                     EIRWNETILTAPGDFLIPPRCAYTAQVPRLFSNTLRNNILLGMQRSDEEIYKATKLAV
                     MDRDLEQLDDDLETMVGARGVKLSGGQAQRTAAARMFIRESELVVFDDLSSALDVETE
                     SQLWERIFAAGNQLTCLVVSHRRPLLRRADHIIVLKDGKVESEGTLDHLLTTSQEMRE
                     LWKLEE"
     gene            1590..2018
                     /locus_tag="PL000_19230"
     CDS             1590..2018
                     /locus_tag="PL000_19230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC91074.1"
                     /translation="MTQPASTEMIHAARERFNSAIATKDADIITSLLAPNYHLITGRS
                     AQFHGAEEEGIRWSELFRTDPTAIYRRTPREVTTNESWGLAQEIGNWQGSYTAEGILV
                     HASGVYAAKWQRAKSGEWMLQAEVFTTLTCEGPCDPPDPI"
     gene            2059..3480
                     /locus_tag="PL000_19235"
     CDS             2059..3480
                     /locus_tag="PL000_19235"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNC91075.1"
                     /translation="MDIITGKILKLNNWPDGRIIGLAKDAAAKLMEQGMERDAVLAQL
                     EAVRQNPGSFLADAVTADLARECIRTMQKDEPKPETLREGPLPFPIWGRDNIDDEAVK
                     QMNNAMRLPVTVTGALMPDAHVGYGLPIGGVLATENVVIPYAVGVDIACRMRLSVYEV
                     SPNLLGQKKGLFEESLWNETAFGMGAKWTGAKKAQHEVLDDSAWFATRQLQTLKDTAM
                     NQLGTSGTGNHFVEWGSFKLHEDTFGLKAGQYLALLSHSGSRGVGAKIADRFSKLAME
                     KHPNLDKSVKHLAWLSLDSEEGQEYWLSMELAGRFASANHYIIHKRVAAAIGLKEVAA
                     VENHHNFAWHETLADGRKVIVHRKGATPAGKGVLGVIPGSMGDAGYVVRGRGVENSLS
                     SASHGAGRKMSRRVAIDSISKSARDVYLKERGVTLLGGGLDESPQAYKQIEDVIAAQN
                     DLVEIVGKFSPKIVRMADEPGDI"
     gene            3561..4181
                     /locus_tag="PL000_19240"
     CDS             3561..4181
                     /locus_tag="PL000_19240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC91076.1"
                     /translation="MNRKLPAKFQNLLLRYSQEVNNVNREAIEAELWNEYGSERAIFV
                     LDMSGFSLLTRKYGIIHYLSMVRRMQLVSEPIITSFHGKVIKFEADNCFAYFPDTLPA
                     IQAAIALQHAFDASNLLTPDDFDVHISCGIDFGKILVIGNKDCFGDAVNRACKLGEDT
                     AAAGEILVTQKAMERVSPESGVKSRPLNVSASGITIPSHTIDYRGK"
     gene            4266..4712
                     /locus_tag="PL000_19245"
     CDS             4266..4712
                     /locus_tag="PL000_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91077.1"
                     /translation="MKQSAFYFIVVSVLILFLAACTPPATATQPPAEASPVPSETPTA
                     IPSATATPSPLYIVINIDFAYLRSGPDVDYPTISDAYIRGTKMEVLSKYKDWFYVKAP
                     DEKIGWLYLEWIDASGIDLNIIPTPSFLPEPPVRPTPKPTNPGYPN"
     gene            4788..5714
                     /locus_tag="PL000_19250"
     CDS             4788..5714
                     /locus_tag="PL000_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91078.1"
                     /translation="MTLPVAKSLREFLKNLIDYAGLFPPASLDLASALDEYKAYLPSA
                     DNWMLGRFIISAADLSKLPNLSDAWHFSVLGRSGKNADEFTQNLENDLNDIRSFHQHQ
                     PAFADVFEVRLPSEATPDLLKYANEKITTQNKLTAYYEIPITPAWRENMTATIIALSE
                     SNQTANLHNGFKLRCGGVVADAFPSVEQIAHALLLCRDHHVPFKATAGLHHPIRHYNE
                     SVQTKMHGFVNVFGAAILAQAHNLNTEQTVAILNEEDPQHFRFTESHFTWRDFSVTAN
                     QIADSRRSQFISYGSCSFDEPREDMQKLTWLS"
     gene            5760..7007
                     /gene="fahA"
                     /locus_tag="PL000_19255"
     CDS             5760..7007
                     /gene="fahA"
                     /locus_tag="PL000_19255"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HNC91079.1"
                     /translation="MSKSFIPVSPESHFPIQNLPYGVFSTAEHQGPRVGVAIGEFVLD
                     LAVLEQKGYLKTKHIFNHPNLNSFMALGRSVWHETRATLQALLSDDNPILRDDPDLQK
                     QVLHPMQTVTMHLPAAIGDYTDFYSSKEHATNIGSMIRSKENPLMPNWVHIPIAYHGR
                     ASSVVVSGTDIRRPNGQTIAEGADGPTFGPSRNMDFELEMGFFVGPGNPLGHPIPIEN
                     AEEHIFGLVLINDWSARDIQKWEYQPLGPFLSKNLATSISPWVVTLDALEPFRCENPS
                     QSPTPLPYLQNKGAASYDIKLEVSLQTKQMNSPVTIARSNFRYMYWTMRQQLAHHTVN
                     GCNMRPGDLLGSGTISGPTQDSYGSLMELTWRGEHPLNMPSGEKRTYLEDGDALTLTG
                     WCEGEGYRVGFGEVTGKLLPAHN"
BASE COUNT         1825 a         2117 c         1622 g         1479 t
ORIGIN      
        1 tagtggcaag ctacgggttt tactatgccg acgtgcccat tttcgcagaa atgaacacgt
       61 tgctgcgaaa aaatcttttg cgctacattt tgaaacggcc aggcgcatcc caactccctg
      121 attcacctgg cgaagctgtc agccgcttca aaaaggatgt ggatgaaatt ccgctgttcg
      181 tcattttgat caatgacatc atggtcgggc taatcatcat cgctgtggcg atcatcttga
      241 tgacgcagat cagtccctcc gtaacagtca tggcgctgat cccgctggcg ctcgtcggca
      301 tcgttgcaaa tctggccacg ggccgcatcg agcactaccg ccgatcatcg cggcaggctg
      361 gaggcaaagt gacgggcttc atcggcgagt tctttggcgc cgtgcaggct gtcaaagtag
      421 ccacagccga aaaagatgtc atccatcact ttcacaaact caacgatgaa aggcgtgtgc
      481 tcaccatccg cgaaaaacta ttcgatgaag tgcttggctc gatctaccgt aacatgtcca
      541 cgcttggcac gggcgtcatc cttatcctcg tcgggcaatc catgcgcgag ggcaatttca
      601 cgctcggaga cttttcgctc ttcgtttatt tattgcaaag catgggcgac ctcaccactt
      661 tcggcggcat gctcgcagcc cgctacaaac agcttgacgt ctccgtacag cgcatgtatc
      721 acctgatgga aaatgcaccg ctgaacgcgc tcgttgaaca cagcaaaata gacctggatg
      781 gtcccctgcc cgaagtgatc tatccgacaa aaaccgcatc cgactcactc cgcagcctgt
      841 ccgctgacca tctgacattt cactaccccg attcggtcaa cggcatcgag gatatttccc
      901 tgaagatcaa tcgcggctcg ctgacagtga tcacaggccg aatcggctct ggcaaaacca
      961 cacttctgcg cgccctgctc ggcttgctcc cgatcgaatc tggcgagata cgatggaacg
     1021 aaaccatcct caccgcacca ggtgacttcc tcatcccgcc gcgctgtgcc tacaccgcac
     1081 aggttccacg tttgttcagc aacaccctgc ggaacaacat cctgctcggc atgcagcgca
     1141 gcgacgagga aatttacaaa gccacaaaac tcgccgtcat ggaccgcgac ctggagcaac
     1201 tcgacgacga cctcgaaacc atggtcggcg cacgtggagt caaactctca ggcgggcagg
     1261 cccaaagaac cgctgccgcg cgcatgttta tccgcgaatc tgaattggtc gtcttcgacg
     1321 atctctccag cgcgctggat gtggaaaccg aaagtcaact atgggagcgc atcttcgccg
     1381 caggcaacca attgacctgc ctcgtcgtgt cccatcgccg cccgctattg cggcgcgccg
     1441 atcacattat tgtgctgaaa gacggcaagg ttgaatctga aggcacactc gaccatctac
     1501 tcacaaccag ccaggaaatg cgagagttgt ggaaactgga agaataatta ccgaaaataa
     1561 aagaccattg agcaaaaagg aaaaacatca tgacccaacc tgcttcaacc gaaatgatcc
     1621 acgccgcccg cgaacgcttc aacagcgcca tcgccacaaa agacgccgat atcatcacat
     1681 ccctgctggc gccaaactac cacctcatta cgggccgcag cgcacaattc cacggagccg
     1741 aagaagaagg catacgttgg tctgaacttt tccgaaccga cccaaccgcc atctatcgcc
     1801 gaaccccgcg cgaggtcacc accaacgaaa gctgggggct tgcacaggaa atcggcaatt
     1861 ggcagggaag ttatactgct gaaggaattt tggttcacgc gtcgggcgtc tatgccgcca
     1921 aatggcagcg cgcaaaaagc ggtgaatgga tgcttcaagc cgaagtattc accacgctca
     1981 cctgcgaagg tccctgcgac ccgcccgacc cgatctagca agacatgtca ttccagtgac
     2041 atgtcggaga attaaaaaat ggacatcatc acaggaaaaa tcctgaaact caacaactgg
     2101 cccgacggaa gaatcatcgg gctcgcaaaa gacgccgccg caaaactgat ggagcaagga
     2161 atggaacgcg atgcggttct ggctcaactg gaagctgttc gtcaaaaccc tggcagtttc
     2221 ctcgccgatg cagtgaccgc cgatctcgcc cgtgaatgca tccgcaccat gcaaaaggat
     2281 gaacccaaac ccgaaacttt gcgcgaaggt ccactgccct tccccatttg gggtcgggac
     2341 aatattgacg acgaagccgt caagcaaatg aacaacgcca tgcgcctgcc tgtcaccgtt
     2401 acgggtgctt tgatgcccga tgcccacgtt ggctacggcc tccccattgg cggcgtgctg
     2461 gccactgaaa atgtcgtcat cccctacgcc gtaggcgtgg acattgcctg ccgcatgaga
     2521 ctctcggtct acgaggtttc accgaattta ctcggacaaa agaaaggcct tttcgaagaa
     2581 tcgctctgga acgagaccgc tttcggcatg ggcgcaaaat ggacaggcgc aaaaaaagcc
     2641 cagcatgaag tactcgatga ttcagcctgg tttgccaccc gccaattaca gaccctgaaa
     2701 gacaccgcca tgaatcaact tggcacaagc ggcacaggca atcactttgt tgaatggggc
     2761 agtttcaaac tgcacgaaga cacattcggg ttgaaggctg gtcaatacct ggccctgctt
     2821 tcgcacagcg ggtcacgcgg cgtcggcgca aaaattgccg accgcttcag caagctcgcc
     2881 atggagaagc atcccaacct cgataagagc gtaaaacatc ttgcctggct ctcgcttgat
     2941 tctgaagaag gtcaggaata ctggctttca atggaactcg cgggcagatt cgcttcggcc
     3001 aatcactaca tcatccacaa acgtgtggcc gccgccatcg ggctgaagga agtggctgcg
     3061 gtggaaaacc accacaactt tgcctggcat gaaacactcg ccgatgggcg caaagtcatc
     3121 gtccaccgca agggagcgac acccgcaggc aaaggggtgc tcggcgttat cccaggctca
     3181 atgggcgatg cagggtacgt agtccgcggc agaggggtgg agaattccct ttcttcagcc
     3241 tcgcatggag cgggtcggaa gatgagccgc agagtcgcca tcgattcgat cagcaaatct
     3301 gcgcgggatg tctatctcaa ggagcgtgga gtcaccctcc tcggcggcgg cctcgacgaa
     3361 tctccgcaag cctacaaaca gatcgaagat gtcatcgccg cacaaaatga ccttgtggaa
     3421 atcgttggaa agttctcgcc caaaatcgtc cgcatggccg atgaacctgg ggatatttag
     3481 aaaaatgccc tcccaaaact gggagggcat tgtcatggaa ctggcttgcg aaaatgagcc
     3541 tttggttgta ttctcactgc atgaaccgaa aactaccagc caaatttcaa aacctgctct
     3601 tgagatattc gcaggaagtc aacaatgtca accgcgaagc catcgaagcc gagttatgga
     3661 atgaatacgg cagcgaacgc gccatcttcg tgctcgatat gtctgggttc tcattattga
     3721 cacgcaaata cggcatcatc cactatttat ccatggtgcg gcgtatgcag ttggtttcag
     3781 agccgatcat cacttcgttc catggcaagg tcatcaaatt tgaagccgac aactgctttg
     3841 cctacttccc ggatacactt cccgccattc aggccgccat cgccttgcaa catgccttcg
     3901 acgcctccaa cctgctcaca cccgacgatt tcgatgttca catctcttgc ggcattgact
     3961 ttggaaagat actggttatc ggcaataaag attgttttgg cgatgccgtg aaccgcgcct
     4021 gcaaactggg agaagacacc gccgccgctg gggaaattct cgtgacccaa aaagccatgg
     4081 aaagggtatc acccgaatct ggcgtgaaat cgcgcccgct taatgtctct gcctcgggaa
     4141 ttaccattcc gtctcacacc attgattacc gcggtaaata aaaccaggct gcgcccatta
     4201 cttatgtttc attgcgaggt acggaattca actttatgct tgcaaaaaat gtaaaggaga
     4261 attccatgaa acaatccgct ttttacttta tcgttgtatc tgttctaatc ctattccttg
     4321 ctgcctgcac cccacccgca acagccaccc aaccgcccgc ggaagccagc cccgttccgt
     4381 ctgaaacgcc cacggccata cccagcgcca cggccacccc atcaccgctg tacatcgtca
     4441 tcaatattga ttttgcctat ctcaggtcag gccctgatgt ggattacccc accatttccg
     4501 atgcctacat ccgcggcaca aaaatggaag tgctgagcaa atacaaagat tggttctatg
     4561 tcaaagcgcc cgatgaaaag atcggctggc tatatctgga atggatcgat gcctctggga
     4621 tcgacctgaa tatcatcccc acaccttcct tcctgcctga gccgcccgtg cgtcccaccc
     4681 ccaaacccac caaccctggg tatccaaatt aaatacaaca tcccgaaggg aaatcgaaaa
     4741 cccttcggga tgtttctttt ttcaaaacaa gcgataaaat cagccgcatg actcttcctg
     4801 ttgcaaaatc actgcgcgag tttctgaaaa acctgatcga ctatgcaggc ctctttccac
     4861 ctgccagcct cgatctcgct tcagccttgg atgaatacaa ggcgtatctt ccctccgccg
     4921 acaactggat gctcggccga ttcattatct ccgctgctga tttatccaaa ctcccaaacc
     4981 tttccgacgc gtggcatttt tcagtccttg gccgcagcgg aaaaaacgcc gacgaattca
     5041 cgcaaaacct tgaaaacgac ttgaacgaca tacgatcctt tcatcagcat caacccgctt
     5101 ttgcagatgt gttcgaagtg cgcctgcctt cagaagccac accagacctg ctcaaatacg
     5161 ccaacgagaa aatcactacg caaaataaac tgaccgccta ttatgaaatc cccatcaccc
     5221 ctgcctggcg tgaaaatatg acagcaacaa tcattgccct ttccgaatca aaccaaacgg
     5281 ccaacctcca caatggattc aaactccgct gtggaggtgt cgtggcagac gccttcccca
     5341 gtgtggaaca gatcgcacat gccctgctgc tttgccgcga tcatcatgtc ccattcaagg
     5401 caaccgctgg gttgcatcac cccatccgcc actacaacga aagcgtacag acaaaaatgc
     5461 acggattcgt caacgtcttc ggagccgcca tcctcgctca agcacacaac ctgaacacag
     5521 agcaaaccgt ggcgatccta aacgaagaag accctcagca ttttcgtttc accgaatcac
     5581 atttcacatg gagagatttc tccgtcactg ccaatcagat cgccgattct cgaagatcac
     5641 agttcatctc ctacggcagt tgtagcttcg acgaaccgcg cgaagatatg caaaaattga
     5701 cttggttaag ctagccaaaa acgaaaccat ttcccaggcc tccaaaggaa aaccgtttaa
     5761 tgtcaaaatc cttcattcct gtttcccccg aatcccattt ccctattcaa aacctgccgt
     5821 atggcgtttt cagcaccgcc gagcaccaag gtccacgggt tggcgttgcc attggagaat
     5881 tcgtcctgga tcttgccgtg cttgagcaaa aagggtacct caaaacgaaa cacatcttca
     5941 atcaccccaa cctcaattct ttcatggcgc ttgggcgtag tgtatggcat gagacacggg
     6001 caaccttgca agccctgctt tctgatgaca accccatcct gcgcgatgat ccagacttac
     6061 aaaaacaagt cctgcacccg atgcagaccg taaccatgca tctccctgca gccatcggcg
     6121 actacaccga tttttattcc tcaaaagaac acgccaccaa tatcggctcc atgattcgca
     6181 gcaaggagaa tccgctcatg cccaattggg tgcatatccc cattgcatat catggacggg
     6241 caagctcggt cgtcgtcagc ggcacagaca tacgccgacc caatgggcaa accattgctg
     6301 aaggcgcaga tgggcccacc tttggtccaa gccgcaacat ggatttcgaa ctcgaaatgg
     6361 gattttttgt cggccctggt aacccgcttg gacaccccat tcccattgaa aacgccgaag
     6421 aacacatctt cggcctcgta ctcatcaatg actggagcgc gcgcgacatt caaaaatggg
     6481 aataccaacc gctcggcccc tttctctcaa aaaatcttgc cacttccatt tcaccctggg
     6541 ttgtcacact ggatgcgctc gaacccttcc gatgcgaaaa ccccagccaa agcccaactc
     6601 cgctccccta cttgcaaaac aagggcgcgg cctcttacga tattaaattg gaagtctcgc
     6661 tgcaaaccaa gcaaatgaac tcacctgtta cgatcgcacg ctccaacttc cgctacatgt
     6721 attggacaat gcgccaacaa cttgctcacc acacggtcaa cggttgcaac atgcgccctg
     6781 gtgacctgct tggctcaggc acaatcagcg gccccacgca agattcgtac ggaagcctta
     6841 tggaacttac ctggcgcggg gaacacccac tcaatatgcc gagcggcgaa aaacgcacct
     6901 accttgaaga cggagacgcc ctcacactca caggttggtg cgagggcgag ggctaccgag
     6961 tcgggtttgg ggaagttaca ggtaaattac tgcccgccca caattaaatc agtcaaattc
     7021 acagccaacc cgatataact ttc
//
LOCUS       DAOQLC010000171         6987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4499_length_6987_cov_23.499567, whole genome shotgun sequence.
ACCESSION   DAOQLC010000171 DAOQLC010000000
VERSION     DAOQLC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4499_length_6987_cov_23.499567"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PL000_19260"
     CDS             <1..729
                     /locus_tag="PL000_19260"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNC91080.1"
                     /translation="RESHLQAISKLGLSCPVEIVNDGTNGLLAGATRGIGVNVTAGSS
                     NNCRGRNAHGKEGRIVGNGPTFGEYGGGIEIAYRALQAVNYAWIKRNPPTALTKVLLE
                     ATGAKDEADLMEGVSNSQYHIFPFIAIEVINAARNGDAAAQEIVRWAGEELGWLAVSV
                     ARQIEMENDEVEIVQSGSIFEAGEIIIKPMRDVVLKHCPRAKLIRLDGPPVVGAVILG
                     MERAGFDGYAVRDVIVRTAKEIVK"
     gene            751..1935
                     /locus_tag="PL000_19265"
     CDS             751..1935
                     /locus_tag="PL000_19265"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNC91081.1"
                     /translation="MNIGYAQQIITPTLDKPVYLAGFGQNRRAETVHDDLYVRALTIQ
                     TEKITLALVALDLIGFFRHDVLEVVNALKSSAADLQIVIASTHTHHGPDTMGLWGPDD
                     KTCGADQGYMSAIKKKIVDTVIASLSDLKPASAKWTSLHVPGLAKNARNPEILDDELT
                     LLQFSTADDKPLATLFNFPCHPEVLWEHNPNITADYVGYLREEVEKQTGAPCIFFAGA
                     LGGMMTPDVKDHSFEEAEFMGRKLGEEGVKALRAVDSGPLTSVSFEKRIIKAKLTNIL
                     YKLAFGRKLLPDVRDKKGFITSEVNLIKIGGLWLATVPGELLPKLGLQLKAWMKEAGA
                     HVTGVIGLANDEVGYILPVEDFKYPWNPFKPGKHYEETNSIGKDISLKVMNSLKELVS
                     QK"
     gene            1986..3323
                     /locus_tag="PL000_19270"
     CDS             1986..3323
                     /locus_tag="PL000_19270"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91082.1"
                     /translation="MPKIVIIGGGSYTWGPTFMRDLFATPELAGSTLVLHDILQDRVD
                     LVFTLGQKMLHDFGLDFQLEKTLSLEEALQGADFVILTITTGRLESMRPDLEIPAKYG
                     IKQSVGDTTGPGGLARALRNIPVVAEIGRKVMEICPNALFLNYTNPMTVLTRTLAMQG
                     VKVVGLCHEWIGVREKLALIFGTSPEKIQAQIAGVNHLIWVTDLYADGKRVWDELPAI
                     TERILSGEIKVDEEDTSVFTDHAKVKSTLFQLYGALPAAGDRHIAEFFPHFINELTHW
                     GADYDLKLTNIADREALEAFAKTMIESVLNGETDLKPFMEDISTEAANKIIRAVTSGE
                     NYIGIMNLPNVGQVTNLPNIGVVETYGVISNTGAHAIAYGEVPAGVQSVLQKHISNQE
                     LTVQSALTGDWDLALQVLLNDPLSSRLTIPQAKQLLRELLEANKQYLPFFFGE"
     gene            3376..4554
                     /locus_tag="PL000_19275"
     CDS             3376..4554
                     /locus_tag="PL000_19275"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91083.1"
                     /translation="MQPTTRSLFLMRVYYLLWTGAGGFIFPFISLFYAQKGLSGTQMG
                     WLTTIGSLIGLVSAPFIGRISDNAANPRRVLQLCLFGSGVLMLILGQQSLFIIMAVII
                     AVEAFVGAPVFPLSDAQALSISSEKEGFGSIRSWGSLGWAITAFIGGWMVSRAGLVSI
                     FWGYAALYGLCIFILGRIITTPKPASASEEHHLPLGKVARSLFTQHTLLGLTIALSIF
                     WLANGGRHQFETLYMHQLGASEQLIGWAYTYPALMELPIMLWADKLVRKYGSGKILSL
                     SILIEGISLFIIVIHPSIATIMFMRAASGFYYSFYAIASVGYAVENAPEGQGATILSL
                     YYVTLNGILALFNAPLSGAIFDWVGAYPLYIIAAAGTILAWLALVISQRKPAIQLQAS
                     "
     gene            4571..5848
                     /locus_tag="PL000_19280"
     CDS             4571..5848
                     /locus_tag="PL000_19280"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC91084.1"
                     /translation="MSELAILGGPKTRTEAYPQWPVWDQRDIDAVVDVVKSGRWGGYP
                     YPGPKTAELAKKFAELQGGGYAVPMINGTVTMEVALRAAGIGWGDEVIVPAYTFQATA
                     SAPMGAGAIPVIVDVDPNTYCLDAKAAAKAITPKTKAIIPVHLGSNMADMDAIMELAE
                     KHNLIVIEDCAHAHGAKWRGKGAGTIGHFGSFSLQSSKTLTAGEGGILLCRTPEFAAL
                     AASVIDCGRPHALGGGGEDKNGLAMQGGNFRMSELQAAMALVGIERFPAQAKQREEMA
                     AYMDEALSEIKGVRVMKRDERHTTRSFYRYIFAIDPQEFGVEHDVLCGALDAEGVDCW
                     VGYEAMHNYELFQPQRSKLAVPNAFPEYFDFKNMNLPEATRACEHEAVWLDEAIFREG
                     PKGVDDVIAALKKIQKNADELNSAADELRKKYG"
     gene            complement(5945..6469)
                     /gene="tpx"
                     /locus_tag="PL000_19285"
     CDS             complement(5945..6469)
                     /gene="tpx"
                     /locus_tag="PL000_19285"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF001808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNC91085.1"
                     /translation="MTTVRNDVLKIGDKFATLLGDEVSVGQAAPEFTSVVPGWAPVNP
                     IQESKGKVIILSAVPSLDTDVCDRETRRFNEEASKLGDDIIIYTISTDFPMAQKRWCG
                     AAGVDKVKVVSDVLETEFGLKYGLVIKERRFLRRSVFIVGRDGKLTYVKYLPTLGEEP
                     NYDEVINAAKEALK"
     gene            complement(6567..>6987)
                     /locus_tag="PL000_19290"
     CDS             complement(6567..>6987)
                     /locus_tag="PL000_19290"
                     /inference="COORDINATES: protein motif:HMM:NF022606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2938 family protein"
                     /protein_id="HNC91086.1"
                     /translation="FKIPSSNFCIVGRWILYMPEGVFRHSNINAAPKKSAECITGWAT
                     HYLIGMVFAFIFLAVAGNDWLQAPRILPALIFGTVTVSAPFFIMQPAFGQGIAASRAG
                     NPAQARFRSLINHLVFGFGLYLFGLMASWMTWKWTFA"
BASE COUNT         1699 a         1687 c         1906 g         1695 t
ORIGIN      
        1 agagaatcgc atttgcaagc catctcaaag ttgggattat cctgccctgt ggaaatcgtc
       61 aacgatggca cgaacggatt actggctggc gcaacacgcg gcatcggggt caatgtgacg
      121 gcgggttcat ccaacaattg ccgtggacga aacgcacacg gcaaggaagg ccgtatcgtt
      181 ggcaatggcc cgacctttgg tgaatatggc ggcggaattg aaattgccta tcgcgcactg
      241 caagccgtga actatgcatg gatcaaacgc aacccgccca cagcattgac aaaagttttg
      301 cttgaagcca caggtgcaaa agacgaagcc gatttgatgg aaggcgtttc gaacagtcaa
      361 tatcatattt tcccttttat cgcgatcgag gtgatcaacg cggcgcggaa cggtgatgcc
      421 gccgcgcagg agatcgttcg atgggcgggg gaggagttgg gctggctggc ggtttcagtg
      481 gcgcggcaga tcgaaatgga gaatgatgaa gtggagatcg ttcaatcggg gagcatcttt
      541 gaggcaggcg agatcatcat aaagccaatg cgcgatgtgg tgttgaagca ttgcccgcga
      601 gcaaagttga ttcggctcga tggcccgccc gtggtcggtg cggtgatcct cggcatggaa
      661 cgggctggct tcgatggtta tgccgtgcgc gatgtgatcg tgcggacggc gaaggaaatt
      721 gtgaagtaac tgtcaaaata aggattcgat atgaacattg gttatgccca acaaataatt
      781 acgccaacgc ttgacaaacc tgtgtatctt gctggctttg gacaaaaccg tcgcgcagaa
      841 accgtccatg atgatctgta tgtgcgcgca ttgacgattc aaaccgaaaa gatcacattg
      901 gcacttgttg cacttgacct gatcggattt tttcgacacg atgtgttgga ggtcgtaaat
      961 gctttgaaaa gctctgcggc agatttgcaa attgtcatcg cttccacgca tacccatcat
     1021 ggccccgata cgatgggtct ttgggggcct gatgacaaaa cctgcggcgc ggatcaggga
     1081 tacatgtctg caattaaaaa gaagatcgtg gatacggtca tcgcttcgct ttcagacctc
     1141 aaacctgctt ctgcaaaatg gacatccctc cacgttccgg gattggcgaa gaatgcccgc
     1201 aacccggaga ttctcgatga cgagttgacg ttgttgcagt tctccactgc cgacgataag
     1261 ccgctggcaa ctttgttcaa ttttccctgc caccctgagg tgttgtggga acacaaccca
     1321 aatattacag cggattatgt cggctacctg cgggaggaag tggaaaagca aacaggcgct
     1381 ccctgtattt tctttgcagg tgcgctgggc ggcatgatga cccccgatgt gaaagaccat
     1441 tcattcgaag aggcagagtt catggggagg aagttggggg aggagggagt gaaggcgctt
     1501 cgtgcagttg acagtggtcc gttgacatcg gtcagttttg aaaaacgcat catcaaagcc
     1561 aaacttacca acattttgta caaacttgcc tttgggcgaa aacttctgcc ggatgtgcgc
     1621 gataaaaagg gatttatcac cagcgaagtg aatctgatca agatcggcgg gttgtggctg
     1681 gcaacggtcc ccggggagtt gctgccaaag ttgggcttgc agctcaaggc gtggatgaaa
     1741 gaagcggggg cacacgtcac aggggtgatc gggctggcaa atgatgaagt tggctacatc
     1801 ctgcctgtgg aagatttcaa atatccgtgg aatccattca agcctggcaa acattatgaa
     1861 gagacgaatt ccattgggaa ggatatttcg ttgaaggtta tgaattcatt aaaggagttg
     1921 gtgagtcaaa agtaaatagt aattggtaat tgccatttac ccattactca tcaccataat
     1981 aagacatgcc caaaatcgta atcatcggcg gcggttcgta cacatgggga cccactttca
     2041 tgcgggatct tttcgccaca cctgaattgg ctggctcaac ccttgtcttg cacgacatcc
     2101 ttcaagatcg tgtggatctg gtatttacgc ttggtcaaaa gatgctgcat gatttcggtc
     2161 tggattttca actggaaaag acgctttcgc ttgaagaagc cctgcagggt gcagattttg
     2221 tgatcctcac gatcacgact ggcaggctcg aatccatgcg ccctgatttg gagatccccg
     2281 caaagtatgg catcaagcaa tcggtggggg ataccactgg ccctggcggg ttggcgcggg
     2341 ccttgcggaa tattcccgtt gtggcagaga tcgggcgcaa agtgatggag atctgcccga
     2401 acgcgctttt tctgaattac accaacccaa tgaccgttct cacacgaacc cttgccatgc
     2461 agggagtgaa ggtggtcggt ttgtgccacg agtggatcgg cgtgcgcgaa aagctggcgc
     2521 tgatcttcgg tacatcccct gaaaaaattc aggcgcagat cgcaggggtc aaccacctga
     2581 tctgggtcac agacctgtac gccgatggga aacgggtttg ggatgaactc ccagccatca
     2641 ccgaaagaat cttgagcggc gagatcaaag tggatgaaga agatacctcc gtgttcaccg
     2701 accatgcaaa ggtaaagtcc acgctgtttc agttgtacgg cgcattgcct gccgcaggcg
     2761 atcggcacat tgccgaattt tttccgcact tcatcaacga actcactcat tggggtgcag
     2821 attacgacct gaagctgacc aacatcgcag accgcgaggc gttggaagcc tttgcaaaaa
     2881 caatgatcga atcggtgctg aacggtgaaa ccgatttgaa gccgttcatg gaagatatct
     2941 ccacagaggc ggccaataag atcatccgcg cagtgacgag tggagaaaac tatattggaa
     3001 ttatgaatct ccccaatgtt gggcaggtta ctaacttgcc caacattggg gttgtcgaaa
     3061 cttatggagt gattagcaat actggcgctc acgcaatcgc atacggagaa gtcccagctg
     3121 gtgtgcaaag tgttttgcaa aagcatattt ccaatcagga actcaccgtg caatctgccc
     3181 tgacgggtga ttgggacctc gcgttgcaag ttctgctcaa cgatccgctt tcgagcaggc
     3241 tcaccatacc gcaggcaaag caattactcc gtgaactgct tgaagcgaac aagcagtatt
     3301 tgcctttctt ttttggagaa taaacaatgt acggcagcga tcagcgcatc agccgtattc
     3361 atcgttgaaa tttttatgca acccactacc cgctcccttt ttttaatgcg tgtctattac
     3421 ctgctctgga ccggtgcagg cggttttatc ttcccattca tcagcctctt ttacgcacaa
     3481 aaaggattaa gcggcacaca gatgggatgg ctcaccacca ttggcagcct tattggcctg
     3541 gtcagcgcac cttttatcgg gcgcatcagt gacaatgcgg ccaatccgcg ccgtgtgctg
     3601 caactgtgcc tcttcggttc aggcgtattg atgctcatcc ttggtcaaca aagtcttttt
     3661 atcatcatgg ctgtcatcat agcggtcgaa gcgttcgtcg gcgcacccgt ctttccgctt
     3721 tccgatgcac aagcgctcag catctccagc gaaaaggaag gcttcggctc cattcgttca
     3781 tggggttcat tgggatgggc gatcactgcc tttattggcg ggtggatggt cagccgtgct
     3841 ggactcgtct ccattttttg gggatatgcc gccctgtacg gactctgcat cttcatattg
     3901 ggacgcatca tcacaacgcc gaaacctgct tctgcctccg aggaacatca tctccctctt
     3961 ggaaaagtcg cgcggtcatt attcacccaa cataccctgc tcgggcttac catagccttg
     4021 tctatttttt ggctggccaa tggcggacgc caccaatttg aaacactcta catgcatcaa
     4081 cttggtgcaa gcgagcagct catcggctgg gcctacacct accctgcctt gatggaattg
     4141 cccattatgt tatgggcaga caaacttgtc cgcaaatatg gctctgggaa gatcctctcc
     4201 ctgtcaatat tgattgaagg catctcgctg ttcattattg tgatccatcc ctccattgcc
     4261 accatcatgt tcatgcgtgc tgccagtggt ttttattaca gtttctatgc cattgcctca
     4321 gtgggctatg cggttgaaaa tgcgccggaa ggtcagggtg ccaccatcct ctcgctttat
     4381 tacgtgactc tcaatggcat ccttgcgctg ttcaatgccc ccctcagcgg agccatcttc
     4441 gattgggtgg gtgcttatcc actttacatc atcgccgccg cagggacgat ccttgcgtgg
     4501 ctggcgttgg tcatttcaca acgaaaacct gcgattcaat tgcaggcttc ttaaaaaagg
     4561 agaacatcac atgtctgaat tagcgatact cggtggaccc aaaacaagga cggaagcata
     4621 ccctcagtgg ccagtgtggg atcaacgcga tattgatgcg gtggtcgatg tggtcaaatc
     4681 ggggcgatgg ggcggctacc cctacccggg gccaaagacc gccgaactgg caaagaaatt
     4741 tgccgaactg caagggggtg ggtatgcggt gccgatgatc aacggcacgg tcacaatgga
     4801 agtggcgttg cgtgcagcag gtatcggttg gggggatgag gtcattgtgc ctgcttatac
     4861 atttcaggcc acggcatcgg cgccgatggg ggcgggggca atccctgtaa tcgtggatgt
     4921 ggaccccaat acctattgtc ttgatgcaaa ggcggcggcg aaggccatta cacccaaaac
     4981 gaaggccatc atccctgtgc atctcggttc gaacatggcc gacatggacg ccattatgga
     5041 gttggctgaa aaacataacc tgatcgtcat tgaagactgt gctcatgcac acggtgcaaa
     5101 atggcgcggc aaaggtgctg ggacaatcgg tcactttggc tcgttcagtt tgcaatcttc
     5161 gaaaacccta acggcaggcg agggtggtat tttgctgtgt cgtacgcctg agtttgctgc
     5221 tctggctgct tctgtgatcg actgcggacg tccgcatgcg ttgggcggcg gcggagaaga
     5281 taagaacgga cttgcgatgc agggcggtaa ttttcgcatg agcgaattgc aggccgccat
     5341 ggcattggtg gggatcgaac gcttccctgc gcaggcgaag cagcgtgagg aaatggccgc
     5401 ttacatggat gaggccctga gcgagatcaa aggagtgcgc gtgatgaagc gtgatgaacg
     5461 tcacaccacc cgttcgttct atcgttatat ttttgccatt gatccacagg aatttggcgt
     5521 agaacatgat gtgttgtgcg gcgcgcttga cgccgaggga gtggattgct gggttgggta
     5581 cgaggcgatg cacaattatg aactgttcca gccacaaaga tcgaaattgg ctgttccaaa
     5641 tgccttcccc gaatattttg atttcaaaaa catgaacctg ccggaagcca cccgcgcctg
     5701 tgagcatgaa gctgtatggc tggacgaggc gatcttccgc gaaggtccaa aaggtgtgga
     5761 tgatgtgatc gctgcgctca aaaaaatcca aaagaatgca gatgaattga attccgccgc
     5821 agatgagttg agaaaaaagt acggctaaga cagccgtctg aaaaagtgcc aggttgattt
     5881 cgctttggaa acaaaaaaat tccgtctttt tcgagacgga atttttttgt aatttggtcg
     5941 aaccttactt tagggcttct ttggcggcgt tgatgacttc gtcgtaattc ggttcctcgc
     6001 caagggtggg gaggtatttg acgtaggtca acttgccgtc gcgccctacg atgaagaccg
     6061 agcggcgcag gaagcggcgt tccttgatga caaggccgta cttcaagcca aattcggtct
     6121 caagtacatc ggaaacgact tttaccttgt ccacaccggc ggcgccgcac cagcgttttt
     6181 gtgccattgg gaaatcggtg ctgatggtgt agatgataat atcatcgccc agttttgagg
     6241 cttcttcgtt gaatcggcgg gtttcgcggt cgcatacatc ggtatccaga gagggtacgg
     6301 cagaaaggat gatcaccttg cctttggatt cctggatcgg attgacaggc gcccagcctg
     6361 gaacaaccga ggtgaattcg ggcgcggcct gccccacact tacttcatcg ccaagcaggg
     6421 ttgcgaattt atcgccaatt ttgagtacat cgttacgaac agtggtcatt acagatctct
     6481 ccttgagttg gttatggaat ggataggtgg taactgtaaa atcttacaaa ttccgggcgg
     6541 attttaccgc aatacttttg tgtatttcaa gcgaaagtcc atttccatgt catccagctt
     6601 gccatcaacc cgaaaaggta aagaccgaag ccaaaaacaa ggtgatttat caaactgcga
     6661 aaccgcgcct gagcaggatt ccccgcccgc gatgctgcaa tcccctgtcc aaacgctggc
     6721 tgcataatga agaatggagc agatacggtg accgttccaa agataagagc aggaagtatc
     6781 cttggggctt gcagccagtc gttacctgca actgccagaa aaatgaaggc aaataccatg
     6841 ccaatcaggt aatgagtggc ccatcctgtt atgcattctg cgctcttttt tggtgcagca
     6901 ttgatattgg aatgcctaaa aacaccctct ggcatatata ggatccagcg cccaacgatg
     6961 caaaaattgg atgatgggat tttgaag
//
LOCUS       DAOQLC010000172         6987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4500_length_6987_cov_18.869446, whole genome shotgun sequence.
ACCESSION   DAOQLC010000172 DAOQLC010000000
VERSION     DAOQLC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_6987_cov_18.869446"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..1467)
                     /locus_tag="PL000_19295"
     CDS             complement(205..1467)
                     /locus_tag="PL000_19295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91087.1"
                     /translation="MANVQKLLKISPDKLKAFNSILLDPNSQIMKDFLAVVAKYGTPE
                     EINKKHRAARKMENLFKIVQERNPEAIKDLNWLIQQRDKGAFISVADYRKKVLGNAAK
                     KMKFKDKYAVTLEVSALQYFPWVRVMAEEAIANQTLMPGRYIAVRKMKESEADGDLPA
                     IVAALDIIGASFVETLDTKGTDGSNPHLGGPATITGYFGGIGQPNEHALMWLDEFLYY
                     YTNYGVQHVLNFNAGTILLAFLLYRLGVNIQFKISVFFGADNPYNALWILTMAKLLSR
                     DDGTSPLIGFNWSNSVNNQTMELTADFRKALDLEKVVRFEHHITEPQKSIVLQPYNRR
                     AELVELADHVANIAAKHEGGDPEVDVTRAHQSDILDYFREKKEVLASGDWDNLQRNYL
                     DKVDACNRTAWALTEKGLSFIAAKNLHK"
     gene            complement(1526..2401)
                     /gene="panB"
                     /locus_tag="PL000_19300"
     CDS             complement(1526..2401)
                     /gene="panB"
                     /locus_tag="PL000_19300"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC91088.1"
                     /translation="MTETRKKITLPYLFKKVEDKQPITWLTCYDYPTAYLQEQAGIEM
                     ILVGDSLGMTMLGYDSTLPVKMDDMIRHAQAVRRGAPTAFVVGDMPYMTYQDSVENAI
                     RNAGRFMSEAGCDAIKLEGGAAMANRIKGIVDAGIPAIGHLGLTPQSVSALGGFRLQG
                     KSATLAKKILDDAKALEEAGAFCILLELVPDRLCKLITERAENCIIMSLGSGPDAHGQ
                     LLIYHDMFGLYPKFKPKMAKVYGNAGEVILNGLKGYHDEVIGGQFPEKDNYFGMPDEE
                     FEELKKMVEGGQLHR"
     gene            2524..4320
                     /locus_tag="PL000_19305"
     CDS             2524..4320
                     /locus_tag="PL000_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91089.1"
                     /translation="MNILRLRSFVTAYLPQIFFSALNLGALTALNLYVPRIIRDVIDD
                     GLVGGDTGFLLRSALILLGLGLTAAALTALQRYLSEWIGSRVGYDIRNKLYNHIQNLS
                     FSYHDHAQTGQLISRCIEDVRAVQNFIGSSVVELTQLVFVLFGALFIMFSANWQLTLI
                     AVIPIFPLVWMTFKFGDRVTHLFFNVDNLLGELSARLQENVTGVQVVRAFAREPHEVK
                     RFDIVNRAYYNARLKVIGEWAKVMPSTDFLVTLCTILILWFGGQMVLNGTMTVGEIVA
                     FNAYVMMLAAPAQQLTWLVNAAGEADAGAKRVLEVLDAQPEIASPVAAVQLPSLRGEV
                     EFKNVSLKYLDEKRSSLTDVHLSVKPNQLVALIGQTGSGKTSLINLIPRFYDVSDGAV
                     LVDGEDVRGVDLVSLRRQIGIVLQTSLLFSDTIRENIAYGRPDATEEEVIAAAKAAQA
                     HEFITGFPEGYKTIVGERGVTLSGGQRQRVAIARALLMNPRILILDDSLSSVDTQTEK
                     LIQEALDNLMEGRTTFVIAHRLSTVRRADLILVMDKGHIVEQGTHTELLKRGGLYKEI
                     HDLQLAQNADPAEVTLFEARPEKAVYHSRDLN"
     assembly_gap    4431..4530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4531..6325
                     /locus_tag="PL000_19310"
     CDS             <4531..6325
                     /locus_tag="PL000_19310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91090.1"
                     /translation="MTTITPPTYVTQSEEFYDKPLDPNIAKRLIGFVAPYQWQLLFSA
                     FLMLVAVLSSVMGPYLVKIAIDDGLVAGNYTVLRNVVLFYLAAAIIRWGFIYWRVNIM
                     ARVGQSVIYDMRKLLFEHLQSLSLGFFSRYSVGRVITRVINDVETLREFITWAVLAIA
                     RDLFALVAIIVAMLALNVKLSLLTFVTIPFMILATVVYRRTARIAYRKVRAAVSWVNS
                     VLAENINGIRVVQAFSRQSHNYDSFRNYTNRYYLQTAINAAKVAASFLPVVDVLGALA
                     TAAVVYVGGTAVLGESITAGVLVAFVLYIDRYFEPIRDLSRRFDTLQSAMAGGERLLE
                     LLDTQVEVRDADNAHAMEPIHGGVRFERVSFHYSDDPTLVLDGIELDVKAGETVALVG
                     ETGAGKTTIVKLLARFHDPTDGCVLVDGVDLRTVTQRSLREQMGMVLQDPFLFNGSVR
                     ENILFGRLNASDEEVIAAAQAVDAHDFIVGLKNGYDTSVEEGGIMLSVGQRQLISFAR
                     ALLADPRILILDEATSSVDTQTEQVIQKALARLLKGRTSFVIAHRLSTITSADKIVVI
                     EGGRIVEQGTHAELLEKRGKYFELYQTGFQE"
     gene            6332..>6987
                     /locus_tag="PL000_19315"
     CDS             6332..>6987
                     /locus_tag="PL000_19315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC91091.1"
                     /translation="MTNTNATQNEFLYRMSNSLRRGLADVQKNVNDLYRLEELEDYIG
                     VKDSPMEYIQAALAPMIAQVSDFQDYAETVTGRMALNLEEVDIHALLDGVLTIADQLV
                     EQKSGVTLEDEIEDDLPLIEADATRLSQALLNLIHNAVKFTESGTVTVLVSREPEDIL
                     FEVRDTGMGIAEENQELVFQPFETILEDKDDPRLGFGLGLKIVEHIVDLHDGETWFES
                     "
BASE COUNT         1423 a         1765 c         1936 g         1763 t
ORIGIN      
        1 tcttgcataa gttgcacgga atgtttttgg aaaaccattt tgaaccgcgg agacactgag
       61 gcggtgcgga gcctcctgcg gacggagttt taattgtttt ctcggtgtct ccgtggctca
      121 gtggttaatc tcttttcaag agatctattc aaaattcaca gctgattccc taagtttgga
      181 gcgtatcacg tttgcagttt tcgtttactt gtgtaaattc tttgcggcga tgaaacttag
      241 ccctttttct gtcaacgccc aggcggtgcg attgcaggca tccaccttgt cgaggtagtt
      301 tcgttgtaga ttatcccaat cacccgaggc aaggacttcc ttcttctcgc ggaagtaatc
      361 cagaatgtca gactgatgtg cgcgggtgac atccacttcg gggtcgccgc cctcgtgttt
      421 tgcggcgata ttggcgacgt gatcggcgag ttccaccaat tcagcgcggc gattgtatgg
      481 ctgcagcaca atgctcttct gtggctcggt gatgtgatgc tcaaagcgga cgaccttttc
      541 caaatccagt gctttgcgga aatcggcggt caattccatg gtctggttgt tgaccgagtt
      601 tgaccagttg aatccgatca gtggactggt tccatcgtca cggctgagca gtttggccat
      661 ggtcagaatc catagggcgt tgtagggatt atccgcgccg aagaagacgg atatcttgaa
      721 ttggatgttc acccccagac gatacagtaa aaaggccagc aggatcgtgc ctgcgttgaa
      781 attcaacaca tgctgaacgc cgtaatttgt gtaatagtac aggaattcgt ccagccacat
      841 cagcgcgtgt tcattgggtt ggccgatgcc gccaaagtag cctgtgatgg tggctggccc
      901 gcccaggtgt ggattcgacc cgtcggtgcc tttggtgtcg agtgtttcga caaaggacgc
      961 accgatgata tccagagcgg cgacgatggc aggcaggtct ccatcggctt cagattcctt
     1021 catcttgcgg acggcaatgt agcggccagg catgagggtt tgattcgcga tcgcctcctc
     1081 tgccatgacg cggacccatg gaaaatattg cagcgcagaa acctccagcg tgacggcata
     1141 tttgtctttg aatttcatct tctttgccgc gttgccgagc actttcttcc gatagtcggc
     1201 tactgaaata aaggcgcctt tgtcacgttg ttgaatgagc caattcagat ccttgatggc
     1261 ctcggggttt ctttcctgca cgatcttgaa gagattttcc attttgcggg cggcgcggtg
     1321 ctttttgttg atctcttcag gtgtgccgta ctttgccacc acggcaagga aatctttcat
     1381 gatctgtgaa ttggggtcaa gaaggatcga gttgaatgct ttgagtttat cgggcgagat
     1441 cttgagaagt ttctgtacgt ttgccattct tgctccttgt gtaagcaggc ggcagttatg
     1501 accagaatga ctgccgcctg ttctgttatc gatgcagctg gccgccttcg accatcttct
     1561 tcaactcttc gaattcttcg tcaggcatgc cgaagtagtt gtctttttcg gggaattgtc
     1621 cgccaatcac ctcatcgtga taccctttga gtccgttcag aatgacttcg ccagcgtttc
     1681 cgtaaacctt tgccattttt ggtttgaatt tgggatacaa gccgaacatg tcgtgataga
     1741 tcagtaattg accatgcgca tcaggccctg agccaagcga catgatgatg cagttctcgg
     1801 cgcgttcggt gatgagcttg cacaaacggt caggtaccaa ctcaagcaga atgcagaacg
     1861 cgcctgcttc ttcgagcgct tttgcatcgt ccaggatctt ttttgccagc gtggcagatt
     1921 tcccttgtaa gcggaaccca cccagagcgg agacagattg cggagtcagc ccaaggtggc
     1981 caatggctgg gatgccagca tccacgatcc ctttgatacg attcgccatt gcggctccac
     2041 cttcgagctt gatggcatcg catccagctt ctgacatgaa tcggccagca ttgcggatcg
     2101 cgttctccac agaatcctga taggtcatgt acggcatatc gccgaccaca aatgcggttg
     2161 gtgcgccgcg cctcacggct tgtgcatggc ggatcatgtc atccattttg acgggcaggg
     2221 tggagtcgta gccgagcatc gtcatgccaa ggctgtcgcc gaccaaaatc atttcgattc
     2281 ccgcctgttc ctgcagataa gcagttgggt agtcgtaaca ggtcagccag gtgatcggct
     2341 gtttatcttc gacctttttg aacaaatacg gcagggtgat tttcttgcgc gtttcggtca
     2401 ttcttgctcc tttttggatg ggtgtgattt cagtatataa gcgggaggca ggcgggtaca
     2461 actgacgaaa atcatgatgt ttgattgatt ttctcgtggt atccttaaca ccccgcataa
     2521 accatgaaca tacttagact ccgctccttt gtcactgcct atttgccgca gattttcttt
     2581 tctgcgctca atttgggcgc ccttaccgcc ttgaacctgt atgtgccgcg catcatccgt
     2641 gatgtgatcg acgacggact ggtcggcggc gatactggat tcctgctccg ctcggctctg
     2701 atcttgctcg ggctgggtct gaccgccgcg gcattgaccg cccttcagcg ttatctttcc
     2761 gaatggatcg gttcgcgtgt ggggtatgac attcgaaata agttgtacaa ccacatccag
     2821 aatctttcgt tttcatacca cgaccatgcc cagacagggc agctcatctc ccgctgtatc
     2881 gaagatgtgc gtgccgtgca gaacttcatc ggaagttctg tggtggagtt gacccagttg
     2941 gtcttcgtcc tgtttggggc gctgttcatc atgttcagcg ccaattggca gttgaccttg
     3001 atcgcggtca ttccaatctt cccgctggtg tggatgacct tcaagttcgg cgaccgtgtg
     3061 acccatctct ttttcaatgt ggataacctg cttggggaac tttctgcacg cctgcaggaa
     3121 aacgtgacag gtgtgcaggt tgtacgcgcc tttgcccgtg agccgcatga ggtcaagcgt
     3181 tttgatattg tcaaccgcgc ctattacaat gcccgcctca aggtgattgg cgaatgggcg
     3241 aaggtgatgc cttccaccga tttccttgtc acgctgtgta ccattctcat tttgtggttc
     3301 ggcggacaaa tggtcttgaa tggcacgatg accgtcgggg agatcgttgc cttcaatgcc
     3361 tacgtgatga tgttggccgc ccccgcccag cagttgacct ggctggtaaa tgccgctggt
     3421 gaagcggatg caggtgcaaa gcgcgtgctt gaagtgttgg acgcgcagcc cgagatcgcc
     3481 tcgcctgtgg cggcggtgca gcttcccagc ctgcgcggtg aagtggaatt caaaaacgtc
     3541 tcgctcaaat accttgatga aaaacgttcc tcccttaccg atgtccatct gagcgtgaag
     3601 cccaaccagc tggttgcgtt gatcggccag acgggcagcg gaaaaacttc gctcatcaat
     3661 ctcattccgc ggttttacga tgtgagcgat ggcgctgtgc tcgtggatgg cgaggacgtt
     3721 cgtggcgtgg acctggtttc cctgcgcagg cagatcggca ttgtgctgca aacctccctg
     3781 cttttctccg ataccatccg tgagaacatt gcctacggcc gacccgatgc caccgaagag
     3841 gaagtcattg cggcggcgaa agcggcgcag gcccatgaat tcatcacagg tttccctgag
     3901 ggctacaaga ccattgtagg cgaacgcggt gtgacccttt caggcgggca gcgtcagcgg
     3961 gtggcaatcg cccgtgcctt gctgatgaat ccgcgcattt tgatcctgga tgactccctt
     4021 tccagcgtgg atacgcagac cgaaaaactg attcaggaag cgttggataa cctgatggaa
     4081 ggccgcacca cctttgtcat tgcgcatcgc ctctccaccg tccgccgtgc cgacctgatc
     4141 cttgtgatgg acaagggaca tattgttgaa cagggcaccc ataccgaatt gctaaaacgc
     4201 ggcggcctgt acaaggaaat tcacgatctg cagctggctc aaaatgccga ccctgccgag
     4261 gtcacgttat ttgaagcgcg ccctgaaaaa gccgtctatc attccagaga cctcaattga
     4321 tccacccaca acctttaccc ttcaacctat aacctataac ctgaaccctt cacctcatga
     4381 caaccattac ccctccaact tacgttaccc agtcggaaga attctacgac nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn catgacaacc attacccctc caacttacgt
     4561 tacccagtcg gaagaattct acgacaaacc gcttgacccg aatattgcca aaaggctgat
     4621 cggatttgta gcaccgtatc aatggcaatt gctgttttcg gctttcctga tgctggtggc
     4681 cgtactttct tctgtgatgg ggccgtacct tgtcaaaatt gcgatcgacg acggccttgt
     4741 ggcagggaat tacaccgtgc tgcgaaacgt cgtgttgttt taccttgcgg cggcgatcat
     4801 ccgttggggc ttcatctatt ggcgcgtgaa catcatggcg cgggtggggc agtcggtgat
     4861 ctacgacatg cgcaagttgt tgttcgagca tttgcagagc ctttcgctgg gattcttcag
     4921 ccgttactcg gtggggcgcg tgatcacgcg tgtgatcaac gatgtggaga ccctgcgcga
     4981 attcatcacc tgggcggtgc tggccattgc gcgtgacctg tttgcgctgg ttgcgatcat
     5041 cgtggcgatg ctggcgctca atgtaaaact atccctgctt accttcgtga ccatcccgtt
     5101 catgatcctt gccacggtgg tctatcgccg caccgcgcgg attgcctatc gcaaggtgcg
     5161 cgcggcggtt tcatgggtga actcggtgct ggcggaaaac atcaacggca tccgtgtggt
     5221 gcaggcgttc agccgtcagt cgcataatta cgattcgttt cgcaactaca ccaaccgcta
     5281 ttatttacag accgcgatca acgccgccaa agtggccgcg tcctttttgc ctgtggtgga
     5341 tgtgcttggc gcactggcaa ccgcggcggt ggtatatgtg ggcggcacgg cagtgttggg
     5401 tgaatcgatc acggcggggg tgttggtggc ttttgttttg tacattgacc gttacttcga
     5461 gccgatccgt gatttgagcc gccgttttga caccctgcaa tcggccatgg ctggcggcga
     5521 gagactgctg gagttgctcg atacgcaggt ggaggttcgg gatgcagaca atgctcatgc
     5581 gatggaaccc attcacggcg gagtccgatt cgagcgggtt tccttccact attccgatga
     5641 tccaaccctt gttttggatg ggatcgagct tgatgtgaag gcaggtgaga cggtggcgtt
     5701 ggtgggggag acgggcgcag gcaagacgac gattgtgaag ctgctcgccc gcttccatga
     5761 cccaaccgat ggctgcgtgc tcgtagacgg tgtggacttg cggacagtga ctcagcgttc
     5821 gctgcgtgaa caaatgggca tggtgctgca agaccccttc ctgttcaatg gcagtgtgcg
     5881 cgagaatatt ttgtttggac gtttgaatgc cagtgacgaa gaagtgatcg ctgccgcgca
     5941 agccgtagat gcgcatgatt tcatcgtggg tttgaagaac ggatacgata cttcggtgga
     6001 ggagggcggc attatgctca gcgtggggca gagacagttg atctcgttcg cgcgtgcgct
     6061 gctggccgac ccgcggatct tgatcctcga tgaggccact tcttcagtcg atacacagac
     6121 cgagcaggtg attcaaaagg cgctggcgcg tttgttgaag ggacgaacct ccttcgtcat
     6181 tgcgcatcgc ttgtccacca ttaccagtgc ggataaaatc gtcgttatcg aaggcggcag
     6241 gatcgtggag cagggcactc atgctgaact gctcgaaaaa cgcggcaaat attttgaact
     6301 atatcaaact ggctttcagg agtagaaccc catgaccaat accaatgcga cccagaatga
     6361 atttttgtat cgaatgtcca actcgctgcg cagggggctg gctgatgtgc agaagaacgt
     6421 caatgacctg taccgtttgg aggagttgga ggattacatc ggcgtgaagg attcgcccat
     6481 ggagtacatt caggcggcgc tggctccgat gatcgcgcag gtgagtgatt ttcaagatta
     6541 cgccgagacc gtgacaggca ggatggcgct caacctggaa gaggtggata tccatgcgct
     6601 gctcgatggg gtgttgacca ttgccgacca gttggtggag caaaagagcg gagtgaccct
     6661 ggaagatgag atcgaagatg acctgcctct gatcgaggca gacgccacac ggctttcgca
     6721 ggcgttgttg aatttgatcc acaatgcggt gaaattcacc gaaagcggaa cggtcacggt
     6781 gttggtttca cgcgagcccg aagacattct ctttgaggtg cgcgatacgg gcatgggcat
     6841 cgccgaggag aatcaagaac tggtctttca gcctttcgag accattttgg aagacaagga
     6901 cgatccgcgc ctcggctttg ggctggggtt gaagatcgtg gaacacattg ttgacctgca
     6961 cgacggcgag acctggtttg agagtga
//
LOCUS       DAOQLC010000173         6974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4514_length_6974_cov_26.159416, whole genome shotgun sequence.
ACCESSION   DAOQLC010000173 DAOQLC010000000
VERSION     DAOQLC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4514_length_6974_cov_26.159416"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PL000_19320"
     CDS             <1..836
                     /locus_tag="PL000_19320"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HNC91092.1"
                     /translation="HHELSTYGIGRELTKSQWMHLSRQLVERGYLYQEPAYRVLSVTD
                     QGLEMLKSREQVFGQINEARKSERASARFKPSPDSEYDRSLFAQLRNRRKELADEAGL
                     PPYVVFSDKTLVEMAMYFPQSRESLLNISGVGKVKFERYGIPFLEIIKEYCRANKIDE
                     KFKAPAREKDDTGRRYMAVGDAYNAGESVQSLMKQYNVSADTILNHLARFIMAGNPLR
                     SAEDLIALSNLEPDQQMAVFKAFDKHGSDMLKPVYDAMNGKVNYDELRILRLCYLCEK
                     R"
     gene            839..1732
                     /locus_tag="PL000_19325"
     CDS             839..1732
                     /locus_tag="PL000_19325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNC91093.1"
                     /translation="MFSNYITGLKQRVVKVAQTVNERANGFPWVLYNTVNKTLMPDTA
                     IMASAIAYISLFSLFPLILLSISLASFSFGFAMMDQHETIKQLEFVAPALGQLLGQNI
                     DQVIRTRGPVTGFALLSLIWSASTIFYTLTHTLNGIWNVEQRHSSWRIRGVAILFVLV
                     FIGPTLFLISLAGSVIANIRFWLPDLILPFGYGISLLLTLLLDVLLFMILYMILPHGS
                     ASWREVLPGASGAGLLWEFAKRTFLSFVSTYISITNLVYGTVATIIAFLLWAYISSLI
                     FIFGAYLSVSYWQLKQNPKTG"
     gene            1837..>6974
                     /locus_tag="PL000_19330"
     CDS             1837..>6974
                     /locus_tag="PL000_19330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HNC91094.1"
                     /translation="MSFAHLHVHTEYSLLDGFSNIKKLVHRVKEMEMPAVAITDHGTM
                     FGVIDFYKAAKAEGIKPIIGLEAYMAARGMKDKDSKLDRSSYHLLLLAENETGYKNLL
                     KISSAAQLEGFYYYPRIDRDFLAAHSEGLICTSGCMSAEIPRALLQDNPEEAVRRMNW
                     YYDVFGKDNFFVELQQHNIKEITDLNKKLLEMGARYEANYIATNDVHYVNQEDARLQD
                     ILLAIQTNSTYNDPERMRMTDDSYFLRSPAEMNRLFAEIPSSLSNTLLIAERCNVDLG
                     FKGYHLPEFPVPDGYTAETYLRSLCEAGAENRYKEDATSSKVKERLDYELSVVHTMGF
                     DAYFLIVWDLCRFARDHGIWYNARGSAAGSIIAYTLGITMVDPLEHALIFERFLNPGR
                     ISMPDIDLDFRDDRRHEMLEYCARKYGDDKVAQIITFGTMGTKAAIRDVARVMEIPLP
                     EVDKVAKLVPFVSGRATTMKDAMEVAEFKDIYEKQKHLRDVIDIAAKMEGTVRNAGTH
                     AAGVVISDKPILEYLPLHRPTSGAEETPIKSVTQFEMGILDSLGMLKVDFLGLITLTV
                     MARACDHIFKRHGIKFDLSNIPIDDPKAFELMGVGQTAGVFQVEGCLSGDTYIGHRTI
                     KELYADFKEREQAGTLGGRELLRTNSCYVDDGHFSLNVINKVIYSGVKPVYRLFADNN
                     TWIKATADHHFFTQRGWVTLGELKPETDTLLFKSNTSRSGKLCVVCGKPLKTATGKSQ
                     HCKSCSAHISSNPSKPEVRERISQSRIGGPAWNKGLTAETAKDTLWIKNLSIYNAAQK
                     GITLEERHGAERAAEIRAKLSARFSGTNNPMYGRPPKETKTYTKSGFREDLGHYVRSS
                     WEADMARVFKYLGWEYQYEPLTFELVKNNGSTITYTPDFYVAEQGVFYEIKGWMDDVS
                     AEKIALFQEQYPEHNLIVVDKTLFAEFQVKYSDLVEWECPQFPTDSEWVNIKGIEYVG
                     EEDTYDLQMQAPGNNFVANGFVVHNSGMTRWLVQMKPKNLDNIIAMVALYRPGPMAFI
                     PDYIARMHGEAEVEYRHPAMEPIFKDTFGIPVYQEQLMRAAVELAGYTPSESDELRKA
                     ISKKKKEDIDKHRNKFVKGAVEKGMDQSTAEAIYTDWEEFARYGFNKSHAADYGVIAV
                     QTAYLKAYYPAEYMAALMSASAGQTEKVALYVADARSMGVPVLAPGINSSNWDFTIED
                     VEDTEKNKITPSIRFGLGAIKNVSENAVEIIVEARKEGKFTDLNDFARRVDLRAVGKR
                     SLECLIKVGALDQFGNRASMLGSLDRIVAISSNHFRAADAGQMSLFGTSTGIIEEIHL
                     PEVKDVEKRDMLNWERELIGLYISDHPLNEFQTQLAQIVSYFAGQLPEASHEEKVRVA
                     GLINVVRPYTTKTGKPMGFVTIEDIQGSIELVLFPKTWEKFREGLNVGQIIIIEGKVD
                     QTNTPPKILVDVIRTEIKMTVAADDPYARQDSTPKPLLPRPAADSPRRPESNKPTVDP
                     VQKATQQPKTGETSTPTAQRTDKPASQPIKESPAHQVKEEAPAYDLDHDMPPPPDNFP
                     DGWDTEWQPTFDDAVIAAKPEPKFKKNEAVTPPLVTRAVAALTQTSEQEQAEDGREAV
                     LPSLYVPLAKEDKDREHPPQQITVILRSTGDRERDKRRIKTLYGTLISYHGRDKFSFQ
                     IFENGKGHLIDFPNDTTRICPELLDRLRKLMNEESWRVEPITFQ"
BASE COUNT         1799 a         1846 c         1773 g         1556 t
ORIGIN      
        1 aacatcatga actttccaca tacggcattg gcagggaatt gaccaagagt cagtggatgc
       61 atctcagccg tcaactggtg gaacggggat atctgtatca ggagcctgcc tatcgggtgt
      121 tgtcggtcac cgatcagggg ctggaaatgc tcaaatcgcg ggagcaggtt ttcggtcaga
      181 tcaatgaagc gcggaagagt gaaagggcga gtgcgcggtt caaaccgtcg cccgattctg
      241 aatatgacag gtccctattc gcccagcttc gaaatagaag aaaagaactg gccgatgaag
      301 cgggtcttcc gccttatgtg gttttctcgg ataagacgct ggtggaaatg gcaatgtatt
      361 tcccgcaaag ccgtgaaagc ctgttgaaca tcagcggcgt gggcaaggtg aagttcgagc
      421 gttatgggat cccgttcctt gagatcatca aggaatattg cagggcgaac aagatcgatg
      481 aaaaattcaa agcgcccgct cgcgaaaagg atgacacagg ccgaaggtat atggcagtgg
      541 gtgatgccta caatgcgggt gaaagtgtgc agtctttgat gaagcaatac aatgtgagcg
      601 ccgatacgat cctgaatcat ctggcgcggt tcatcatggc gggcaatccg ctgcgctcag
      661 cggaggatct gatcgcgctc tcgaaccttg aacccgacca gcaaatggca gtcttcaagg
      721 cattcgataa acacggttca gacatgttga aacccgttta cgatgcgatg aacggcaaag
      781 tgaattacga tgaactgcgg atcttgcggt tgtgctattt gtgtgaaaag agataacaat
      841 gttctcaaac tacatcactg gattgaagca aagggtggtc aaagttgcgc agaccgtgaa
      901 cgaacgcgcg aacggttttc cgtgggtgtt gtacaacacg gtcaataaaa cgttgatgcc
      961 tgatacggcg atcatggctt cagccattgc ctacatttcg cttttttcgc ttttcccctt
     1021 aatcctgttg agcatttcgc tggcaagttt cagttttggt tttgcgatga tggaccagca
     1081 tgaaaccatc aaacaactgg aatttgtagc gcccgcactt ggtcaattgc ttgggcaaaa
     1141 cattgaccag gtcattcgca cccgcggccc tgtgactggt tttgcgctgc tcagtttaat
     1201 ttggtcggcc tcgaccattt tttacaccct cacacacacc ctgaacggca tttggaatgt
     1261 tgagcagcgg cattcttcgt ggcggattcg cggcgtggcg atcctgttcg ttctggtgtt
     1321 cattggccca accctttttc tgatctcgct ggcaggcagt gtcatcgcca acatccgttt
     1381 ctggctgcca gacctgattc tgccatttgg gtatggcatc agcctgctcc tcactttgct
     1441 gctcgatgtg ctgctgttca tgatcctgta catgatcctg ccgcatggct ccgcttcctg
     1501 gcgtgaagtg ttacccggtg caagcggggc gggattgtta tgggagtttg ccaagcggac
     1561 ctttctctct tttgtttcca cctacatctc cattacaaac ctggtgtatg gcacggtggc
     1621 aaccatcatc gcttttttgc tgtgggctta tatcagcagt ctcattttta tctttggggc
     1681 ttatttgagc gtttcatatt ggcagttgaa acaaaaccca aaaacaggct gattttttaa
     1741 gccaaatgca agatcgatga ttgaacatgc gtgctataat gaccacccgc tgaatcactg
     1801 gctactgaat acttaaagaa ccactgatac ccttccatgt ctttcgccca cttacacgtc
     1861 catactgaat attccctgct ggatggcttc tcgaatatca agaagctggt tcatcgcgtc
     1921 aaggaaatgg aaatgcctgc ggtggcgatc accgaccacg gcaccatgtt tggggtgatc
     1981 gatttttaca aagccgccaa agcagaggga atcaaaccca tcatcgggtt ggaagcgtac
     2041 atggcggcac gcggcatgaa ggacaaagac tcaaagctcg atcgttcttc ctatcacctg
     2101 ctgctgcttg cggaaaatga aacaggctac aagaatctgc tcaagatctc ctctgctgcg
     2161 caactggaag gcttctatta ttatccacgc attgaccgtg acttccttgc cgcccattcc
     2221 gaagggctga tctgcacctc gggttgtatg tcggcggaaa ttccgcgcgc gctgctgcag
     2281 gataatcccg aagaagccgt gcgccgcatg aactggtatt acgatgtctt tggcaaagac
     2341 aatttcttcg ttgagttgca gcagcacaac atcaaagaga tcaccgacct gaacaagaaa
     2401 ttgctggaga tgggcgcgcg gtacgaggcg aattacatcg ccaccaacga cgtgcattac
     2461 gtcaaccagg aagatgcgcg tttgcaggat attctgctcg ccattcaaac caactccacg
     2521 tacaacgatc ccgaacgcat gcgcatgacc gacgactcgt acttcctgcg ctcgcccgct
     2581 gagatgaatc gtctcttcgc agagattccc tcctcgcttt caaataccct gctcatcgcc
     2641 gagcgctgca acgtagacct cggtttcaaa ggctatcacc tgcctgaatt ccccgtgccc
     2701 gatggataca ctgcggaaac ctatctgcgt tcgttgtgtg aagccggggc ggagaatcgt
     2761 tacaaggaag atgcaaccag ttcaaaggtc aaagaaagac tcgactatga attgagcgtc
     2821 gtccacacca tgggattcga tgcctacttc ctcatcgtgt gggatctatg ccgcttcgcc
     2881 cgtgaccatg gaatctggta caacgcccgc ggctcggctg ctggctcgat cattgcctac
     2941 accctcggca tcacgatggt ggatccgctc gaacatgcgc tcatcttcga acgctttctc
     3001 aaccctgggc gtatctccat gcccgatatt gaccttgatt tccgcgacga ccgccgccac
     3061 gaaatgctcg aatattgcgc ccgtaaatac ggcgatgaca aagtggcaca gatcatcacc
     3121 ttcggcacaa tggggacaaa agccgccatc cgtgatgtgg cgcgtgtgat ggaaattccg
     3181 ctgcccgaag tggataaggt tgccaaactc gttcccttcg tttcgggccg cgccaccacc
     3241 atgaaagacg caatggaagt ggcggagttc aaggatatct acgaaaagca aaaacacctg
     3301 cgtgatgtga tcgacatcgc cgcaaaaatg gaagggaccg tccgcaacgc gggtacgcat
     3361 gcggcaggtg tggtcatctc tgataaaccc atccttgaat atctgccgtt gcaccgccct
     3421 acttccggtg cggaagagac gccgatcaaa tcagtgactc aatttgaaat gggcattctt
     3481 gattcgctgg gcatgttgaa ggtggacttc ctcggcctga tcacattgac cgtgatggca
     3541 cgcgcctgtg atcacatctt caaacgacat ggcattaaat ttgatttgag caatatcccg
     3601 atcgacgacc cgaaagcgtt cgaattgatg ggggtggggc aaactgctgg cgtcttccag
     3661 gtggaaggat gtttgagcgg tgacacttat attggacatc gaaccatcaa agaattgtat
     3721 gccgatttta aagaacgtga gcaggcaggg acactggggg gacgcgaact cttgcgcacc
     3781 aactcatgtt acgtggacga tggacatttc agtctcaatg taattaacaa ggttatttac
     3841 agcggtgtca aacctgtgta tcgtctgttt gctgataaca acacctggat taaagcaact
     3901 gccgatcatc acttctttac ccagcgtggc tgggttacgc ttggtgagct taaacccgaa
     3961 accgacaccc tgcttttcaa aagcaatacc agccgttcag gaaagttatg cgtagtttgt
     4021 ggaaaaccgc taaaaactgc tactggaaaa tctcaacact gcaagagttg ctcggctcat
     4081 atttcttcga acccaagcaa accggaagtg cgtgaaagaa tctcgcagag ccgcataggt
     4141 ggccctgcat ggaacaaagg tcttaccgct gaaactgcca aagatacgct ttggattaaa
     4201 aacctgagta tttacaatgc tgctcaaaaa ggaattacgc ttgaagagcg gcatggcgcg
     4261 gaacgggcag ccgaaatccg cgccaaactt tcggcccgtt tctccggcac aaacaaccca
     4321 atgtacgggc gtccgccaaa agagacaaaa acctacacca aatctggttt ccgtgaagac
     4381 ttgggacatt atgtccgctc ttcatgggaa gcggatatgg cgcgtgtttt caagtatttg
     4441 ggttgggaat atcaatatga gccattgacc tttgaactgg tcaaaaacaa cgggagcaca
     4501 attacctaca cccccgattt ttacgtcgca gaacagggag tcttttacga aatcaaaggc
     4561 tggatggatg atgtcagcgc ggaaaaaatc gctctttttc aggagcaata ccctgaacat
     4621 aatttgattg tggttgacaa aaccctgttt gcagagtttc aagtcaaata ctctgatttg
     4681 gtcgaatggg agtgcccgca attccccact gatagcgaat gggtaaacat taaaggtatt
     4741 gaatatgtgg gtgaagaaga tacctacgat cttcaaatgc aggcgccagg gaacaatttt
     4801 gtcgccaacg gatttgtcgt acataacagc ggaatgaccc gctggctcgt gcaaatgaaa
     4861 ccgaagaatc tggataacat catcgcgatg gtggcgttgt accgccccgg accgatggcg
     4921 ttcatccccg attacatcgc gcgtatgcac ggcgaagcgg aagtggaata ccgccacccc
     4981 gcaatggaac ccatcttcaa agacaccttt ggcatccctg tgtatcagga acaactcatg
     5041 cgcgccgccg tggaactggc aggctacacc ccctccgaat cggatgagtt gcgcaaagcg
     5101 atctcaaaga agaaaaaaga agatattgac aagcaccgca acaagttcgt caaaggcgcc
     5161 gtggaaaaag gcatggatca atccaccgcc gaagcgatct acacagactg ggaagaattc
     5221 gcaaggtatg gcttcaacaa gtctcatgcc gccgattacg gcgtcatcgc agtgcagacg
     5281 gcctacctca aggcctatta ccctgccgaa tacatggcgg cgctcatgtc tgcctcggca
     5341 ggacaaaccg aaaaagtggc gctctatgta gccgatgcac gcagcatggg tgtgccggtg
     5401 ctggcgcctg gcattaattc ctccaactgg gacttcacca ttgaagatgt ggaagacaca
     5461 gagaaaaaca aaatcacgcc cagcatccgc tttgggttgg gagcgatcaa gaacgtcagc
     5521 gaaaatgcgg ttgagatcat cgttgaagcg cgcaaggaag gcaaattcac cgacctcaac
     5581 gacttcgccc gccgtgtaga cctgcgcgcc gtgggcaaac gctcactgga atgtctcatc
     5641 aaggttggtg cgctcgacca atttggtaat cgcgcttcca tgcttggttc gctcgaccgc
     5701 atcgtcgcca tcagcagcaa tcacttccgc gccgccgacg cggggcagat gagtctcttc
     5761 ggcacatcca caggcatcat cgaagaaatt cacctgcccg aagtaaaaga tgtggaaaaa
     5821 cgcgacatgc tcaattggga gcgtgaactc atcggcttgt acatttccga tcacccgctc
     5881 aacgaattcc aaacccagct tgcgcagatc gtcagctatt ttgcaggtca attgcccgaa
     5941 gccagccacg aagaaaaagt gcgagtggcg gggctcatca atgtggtgcg tccgtacacc
     6001 accaaaacag gcaagcccat gggtttcgtc accatcgaag atattcaagg cagcattgag
     6061 ctggtgctct tccccaaaac ctgggagaaa ttccgcgaag gactcaacgt tgggcagatc
     6121 atcatcatcg aaggcaaagt ggaccaaacc aacacgccgc ccaagattct ggtggatgtg
     6181 atccgcacgg agatcaagat gaccgtggcg gcggatgatc cctacgccag acaggattca
     6241 acacccaagc ctttgcttcc acgccccgca gcagactctc cccgccgccc cgaatcaaac
     6301 aagccgacag ttgatcccgt ccaaaaggcg actcagcagc cgaagacagg cgaaacaagc
     6361 accccaacag ctcaacgcac tgacaaaccc gcgagtcagc cgatcaaaga gtcgccagca
     6421 catcaagtga aagaggaagc gcctgcgtac gatctcgatc atgacatgcc gcccccgcca
     6481 gataacttcc ccgatggctg ggataccgaa tggcagccca cgttcgatga tgctgtgatc
     6541 gcagccaaac ccgaaccgaa gttcaaaaag aacgaagccg tgacccctcc gctggtgact
     6601 cgcgctgtcg cggcgctgac tcaaaccagt gaacaggagc aggccgaaga tgggcgcgaa
     6661 gcggtccttc cttcgttgta tgttcccctg gcaaaagaag ataaagacag ggaacatccg
     6721 ccgcaacaga tcaccgtcat cctgcgctcc acaggcgacc gtgaacgcga taagcgccgc
     6781 atcaagaccc tgtacggcac gcttatctcc tatcacgggc gtgataaatt cagttttcaa
     6841 atttttgaga acggcaaggg acacctgatc gatttcccca acgacaccac ccgcatctgc
     6901 cccgaactgc tcgaccgcct gcgcaagtta atgaatgaag aatcctggcg cgtggaaccg
     6961 atcacgtttc agta
//
LOCUS       DAOQLC010000174         6791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4691_length_6791_cov_24.112827, whole genome shotgun sequence.
ACCESSION   DAOQLC010000174 DAOQLC010000000
VERSION     DAOQLC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4691_length_6791_cov_24.112827"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..471
                     /locus_tag="PL000_19335"
     CDS             169..471
                     /locus_tag="PL000_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91095.1"
                     /translation="MAKKIDGVIEAVRYKNGQVVLVRAYERRGATFSDRVLIERKTLL
                     ERLKKGQQYIVGSREELRASTFKNGKPVLVVTQDGNDLLTTNAAATRDELEETPVF"
     gene            484..1395
                     /locus_tag="PL000_19340"
     CDS             484..1395
                     /locus_tag="PL000_19340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91096.1"
                     /translation="MKIIDKTPLQDASGNINPIARVQGTLKYGLSWFAELEAQKAVIA
                     QLDRLLEKGFVLIRNFTLPNSEIVIPIILIGAGGIWVIYVTKEKGNFEAKGDQWNTLD
                     NNGRAQPAPINFISRVSQLTTAFQKYLKIQKIEIPNPIEAVLIAADPGAQIESVRPIA
                     RVVRSDAIRQFTSTLVQARPVWRTDFIHELADRIIDPTPPAELEPVKAGSPESDLRSQ
                     GGFDEAGQDFNSGSLGFDFEDNSENPTLQIPNNAGQPSSAPRPRPAPAKKGLLGLGNT
                     QVILLAGMLLVECCVLIGFGVLLYTSL"
     gene            1402..2133
                     /locus_tag="PL000_19345"
     CDS             1402..2133
                     /locus_tag="PL000_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC91097.1"
                     /translation="MTTPFLSIIIPAYNEEGRLPRTLERVFAFLEKQPYASEIIVVEN
                     GSSDRTLEVAGEFSKRHTNMQVIREEKRGKGNAVRRGMLEARGEYRFICDADLSMPIE
                     EVQKFLPPAIVDFDLAIGSREAPGAIRYNEPSYRHWGGRAINLLIRILILPGLNDTQC
                     GFKCFRADVVDDLFPRQTLMGWSFDIELLYLARRKRLRIKEIPIHWYFDPDSKVNAVR
                     DALRMIGDIFQIHLNAARGRYDLKP"
     gene            2117..2734
                     /locus_tag="PL000_19350"
     CDS             2117..2734
                     /locus_tag="PL000_19350"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNC91098.1"
                     /translation="MISSPDLSHEQELWPAYRYIAGMDEAGRGALAGPVCVGAVILPD
                     DNPNLTQTLSGVHDSKQLTPRKRIHLIPHIKETALTWGIGFASAAEIDELGIVPATRL
                     AASRAIESLAIFPDFLLTDFRLELPELGVSQAALVKGDQRSLSIAAASILAKTARDEL
                     MRELDAQYPNYRLGIHKGYGTLSHRRAIGQFGFSPIHRKTFQIKK"
     gene            complement(2780..3619)
                     /locus_tag="PL000_19355"
     CDS             complement(2780..3619)
                     /locus_tag="PL000_19355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNC91099.1"
                     /translation="MNPKRLFILFSLLGMVLFGLWLPANAAPNVQQTDPTATALIDGR
                     ILYIVKAGDNCFRVAALHGITVEQLRQLNKELDENCSLTEGQELLISIVSIVGTPTAG
                     PSPTPAPPTVTPTPFTGTTEICVLLFEDTNGDALRQETEPAIAGGAVSVTENNGEYSA
                     AQETFIPADPDVYQGICFLDVPEGNYNITVGIPDNYNPTVDLNYSLDVPAGARAFVAF
                     GAQSRDVVVDPGAEDANESTTSPVFGIIGALLLLGGAGLGYYAWRSGKPESKLAGGNN
                     ILK"
     gene            complement(3698..4117)
                     /locus_tag="PL000_19360"
     CDS             complement(3698..4117)
                     /locus_tag="PL000_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91100.1"
                     /translation="MPNQPLFAGLVVDEDGHPAESSFIGSEPCYVVNDAGFRRHVPAE
                     QIDRAVLAQMAEMMKGSEDILSEQTAKMLGQEDPFSKAIIEQQLKNIDKQFDTLMQAG
                     IPEDMRTYLGMMGFKVVINYHGELVRVEQPGSAGDGE"
     gene            complement(4124..4699)
                     /locus_tag="PL000_19365"
     CDS             complement(4124..4699)
                     /locus_tag="PL000_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91101.1"
                     /translation="MNSAFQFNQYLLKRQVFALAGKFRFFDPGGRLVLYSEQKMFKIR
                     EDIRVYSDESKAQEVLMIKARQIIDFSAAYDVVDSATGQKVGAFRRKGLASIFRDEWE
                     ILDVGDNLIGKLFEDSMGMALLRRILSNLIPQNYDIIIGTNRVADLKQNFNPFTYELN
                     MDFSMDIGRLLDRRMGLAAGILLAAVEGRQG"
     gene            complement(4724..5749)
                     /locus_tag="PL000_19370"
     CDS             complement(4724..5749)
                     /locus_tag="PL000_19370"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNC91102.1"
                     /translation="MMNKLNLSRRDFLKLSASGALGLALSEMGIDRALAAPPSSQGRI
                     TYSGVPLYDAPSFQAKQIHLYGIDLVVELQAEVEGDEGNPFNKAWYQVDGGYTYSGWL
                     QPVETKLQKPVYDIPAEGQLGEIVVPMSETRLIPFTYAKNGPRLYYGSTHWVKKVVVT
                     REEKSIWYEIYDSYVKKSFYVPHYNLRLIPSEELTLLSPDVPESEKLIHVDLGTQMVT
                     AFEGNTMVFQSRCSSGGKGTRTPTGEFLTYHKGPSIHMTNQDEEGVKNYYDLPGVPWC
                     TFFTGYGNAFHGTYWHNDYGRPRSHGCVNLPSEDAKWVYRWSRPNLSPNEDYLHVPGE
                     GTRVQIV"
     gene            complement(5782..>6791)
                     /locus_tag="PL000_19375"
     CDS             complement(5782..>6791)
                     /locus_tag="PL000_19375"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC91103.1"
                     /translation="KLSRRDFLKLGGLGLAGLLTPPLNFHFDDPFAAVQGRVTTRMIW
                     MYDKPSTEATQVTFCHRDKLLNITNAVISDDVNSHNRVWYEIGKEGYVYSGNIQPVRT
                     LLNKPQLNIPKDGMLTEISVPYTDAYEMPDKTSKVAYRMYYETTHWVKAVVTAENGQV
                     WYQVRDDKFDKLYYARSEHMRPMSEAEIAPISPEVENKKIQVQLDEQILVAYENNLPV
                     FVASVSTGGIFRVGTYTTPQGAFITYYKRPSRHMAAGDIAASGFDLPGVPWVQYITEG
                     GISFHGTFWHNDFGRPRSHGCINLSTSTAKWLYRWSAPQVTFTKEFSFGGVGTDVEIV
                     L"
BASE COUNT         1554 a         1742 c         1853 g         1642 t
ORIGIN      
        1 cgatgcgaat cctattgtcc cgactttata aggatggctt tgtaaggatt aggcgtgggt
       61 ttcccataaa aaagcagaga cacggaggac acggaggata actctctgtg actttgtgtc
      121 tctgtaaatt taacccgcgt gtgttaaaat tactcaaaag gaattcccat ggcaaagaaa
      181 attgatggtg tgatcgaagc agttcgttat aaaaatgggc aggttgtgct tgtgcgtgcc
      241 tatgaacggc gcggtgcgac gttttctgac cgtgtattga ttgagcggaa gaccctgctg
      301 gaacgtttga agaagggtca gcagtatatt gtcggctcac gtgaagaatt gcgtgccagt
      361 acgttcaaga atggcaagcc ggttctggtt gtgacacagg acggaaacga cctgttaacc
      421 acaaatgccg ccgccacgcg tgatgaactt gaggaaacac ccgttttcta aaagtggatt
      481 tcaatgaaga tcattgataa gaccccgtta caagatgcca gcggcaatat caaccccatt
      541 gcccgtgttc agggaaccct gaaatacggc ttgagttggt ttgctgaatt ggaagcccaa
      601 aaggcggtga tcgcccaatt ggatcgcctg cttgaaaaag gcttcgtgtt gattcgcaat
      661 ttcaccctgc cgaacagcga aattgtgatt ccgatcattt tgatcggcgc gggcggcatt
      721 tgggtcattt atgtgacgaa agaaaaaggc aatttcgaag cgaagggtga tcaatggaac
      781 accctggata acaacggccg ggcgcagcct gctcccatca actttatcag ccgtgtttcg
      841 caattgacca cagcttttca aaaatatctg aaaatccaaa agatcgaaat tcccaatccg
      901 atcgaagccg tgttgatcgc ggcggacccc ggtgcccaga tcgagtcggt acggcccatt
      961 gcgcgggtgg tgcgcagcga tgcgatccga caattcacat ccacgcttgt gcaggcgcgg
     1021 cctgtctggc gcaccgactt catccacgaa ctggctgacc gtattattga tcccaccccg
     1081 cctgctgaat tggaacctgt caaagcaggc tctcccgaat cggatctgcg ttcacaaggc
     1141 ggtttcgatg aagccgggca ggactttaat tcaggcagcc ttgggtttga ttttgaagac
     1201 aactccgaaa atcccaccct gcagattcca aacaatgccg gtcaaccttc ttcagcaccg
     1261 cgcccacggc ctgcccctgc caaaaaaggt ttgctgggcc tgggaaatac acaggtcatc
     1321 cttttggcgg gaatgctgct cgtggaatgt tgtgtcttga tcggctttgg tgttcttttg
     1381 tacaccagcc tctaaattac tttgacgact ccatttttat ccatcatcat cccggcctat
     1441 aacgaagagg gccgcctgcc tcgtacgctg gagcgcgtgt ttgcatttct tgaaaaacaa
     1501 ccctacgcct ccgaaatcat cgtcgtcgaa aatggcagtt cagaccgtac ccttgaagtg
     1561 gcgggggaat tttcgaaacg ccataccaac atgcaggtca tccgcgaaga gaagcgcggc
     1621 aaaggcaatg cggtcaggcg tggcatgttg gaagcccggg gtgagtatcg cttcatttgt
     1681 gatgccgatc tttccatgcc cattgaggaa gtgcaaaaat ttctcccgcc cgccatcgtt
     1741 gatttcgacc ttgccatcgg ttcacgcgaa gctccggggg ccatccgcta caatgagcca
     1801 tcctaccgtc actggggcgg acgtgccatc aacttgctca tccgtattct tatcctgccg
     1861 gggttaaacg acacccagtg cggtttcaaa tgcttccgcg ccgatgtcgt tgatgacctg
     1921 tttccgcgcc agaccctgat gggctggtcg ttcgacatcg aactccttta ccttgcccgt
     1981 cgaaaacgac tgcgcatcaa ggaaattccc atccactggt attttgatcc cgatagcaag
     2041 gtcaatgccg tacgcgatgc cctgcgcatg atcggcgata ttttccaaat ccacttgaac
     2101 gcagcgcgcg gccgctatga tctcaagccc tgacctttcg catgaacagg aactctggcc
     2161 tgcctatcga tacattgctg gaatggatga agccgggcgc ggtgcccttg ctggaccggt
     2221 gtgtgtaggg gcggtgatcc tgcctgatga caacccgaat ctgactcaaa ccttgagcgg
     2281 ggtgcatgat tccaaacagt tgactccccg caaaagaatc cacctaattc cacacatcaa
     2341 agagacagcc ctcacttggg gcattggttt tgcctccgct gctgagatcg atgaactggg
     2401 cattgtccct gctacgcggt tggccgccag ccgagccatt gaatccctcg cgatcttccc
     2461 cgattttctg ctgaccgatt ttcgcctcga actgcccgaa ctgggcgttt cacaagcagc
     2521 gctcgtcaaa ggtgaccagc gttctttgag cattgcggcc gcatccatcc ttgcaaagac
     2581 cgcccgcgat gaattgatgc gcgaacttga tgcccaatat cccaactaca ggctgggaat
     2641 ccacaaggga tatggcaccc tctctcatcg cagagcgatc gggcaattcg gtttttctcc
     2701 catccaccgc aagacatttc agataaaaaa ataaagtcca aggtcaccag accttggact
     2761 ttattgatcg aacgggggat tatttcaaaa tgttatttcc gccggccagt ttactttcgg
     2821 gcttgcctga tctccatgca tagtatccca ggcccgcacc gcctaacagg agcagtgccc
     2881 cgataatgcc aaatacagga gatgtcgtcg attcgttggc atcttccgca cccgggtcca
     2941 ccaccacatc ccgcgattgc gcaccgaacg ccacgaaagc acgggcaccg gcaggcacat
     3001 caagcgagta gttcaggtcc acggttgggt tgtagttatc cggaataccc acggtgatgt
     3061 tgtaattacc ttcggggaca tccaaaaagc aaatgccctg atacacatca ggatcggcag
     3121 ggatgaaggt ttcctgcgcc gcagaatatt cgccgttatt ttccgtcaca ctgaccgcgc
     3181 ctccggcaat ggcaggttcg gtctcctggc ggagggcatc tccgttggta tcttcaaaga
     3241 gcagaacgca tatttcagtt gtgccagtga acggtgtggg tgtgaccgtg ggcggggcag
     3301 gcgtggggga ggggccagcg gtcggtgtgc caacaattga cacgatgctg attaacaatt
     3361 cctgcccttc ggtcaggctg cagttctcat ccaactcctt gtttaactgg cggagttgct
     3421 ccacggtaat gccatgcaag gctgcgacac ggaaacagtt atcacctgct ttgacaatgt
     3481 acaaaatacg gccatcgatc aaggcggtgg cggttggatc ggtctgttgc acattgggtg
     3541 cggcatttgc aggcagccac aacccgaaca acaccatgcc cagcaatgaa aataagatga
     3601 ataagcgctt tggattcata cgcaaaatta tatcagggtt tatttaacgc aaaaacttcg
     3661 gaggatatcg catcctccga agtttggaaa agagacttta ttccccgtcg cctgcgctgc
     3721 cgggttgttc cacccgcacc aattcaccgt gatagttgat gaccaccttg aatcccatca
     3781 tgccgaggta ggtacgcatg tcttcgggga tgcccgcctg catgagggtg tcgaattgtt
     3841 tatctatatt cttcaattgc tgttcaatga tggcctttga gaagggatct tcctgcccga
     3901 gcattttggc ggtctgttct gaaaggatat cttcactacc tttcatcatt tcagccattt
     3961 gtgcgagtac ggcgcggtcg atctgctcag cagggacatg gcggcggaaa cccgcatcat
     4021 tgaccacgta gcagggttcg ctgccaatga acgagctttc tgcgggatga ccatcttcat
     4081 ctacaacaag accggcaaag agaggttgat tgggcatgag agcttaaccc tgcctgcctt
     4141 ccactgccgc aagcagaatg cccgcagcca gccccatgcg gcggtccagc aggcggccaa
     4201 tatccatgct gaaatccatg ttcaactcat aagtgaaggg attgaaattt tgtttgaggt
     4261 cggctacacg gttcgtgcca atgatgatgt cgtaattttg cgggatgagg ttgctgagaa
     4321 tacgacgtaa caatgccatg cccatgctgt cttcgaagag tttgccgatg aggttgtcgc
     4381 ctacatccag gatttcccac tcatcgcgga aaatggatgc aagccctttg cggcggaatg
     4441 ccccgacctt ttgtccggtg gcgctatcca ctacatcata ggcggcggag aaatctatga
     4501 tctggcgggc tttgatcatc aacacctcct gcgccttgct ttcatctgaa taaacgcgga
     4561 tatcttcacg gatcttgaac atcttttgtt cgctgtacaa caccagcctg ccgcctgggt
     4621 cgaagaagcg gaatttaccc gccagcgcaa agacctgacg cttaagcagg tattgattga
     4681 attggaatgc ggaattcata attagctcct ggtggcaaaa aaactaaacg atttgaacac
     4741 gggtaccttc gcctggtacg tgcaggtaat cttcattggg cgataggttg gggcggctcc
     4801 agcgatagac ccattttgca tcttccgagg gcaggttcac acagccatgg ctgcgcggac
     4861 ggccataatc gttgtgccag taggttccgt ggaaggcgtt gccgtagccg gtgaagaagg
     4921 tacaccacgg tacgccgggc aggtcgtagt aattcttcac gccttcttca tcctgattgg
     4981 tcatgtggat ggagggaccc ttgtgatagg tgaggaattc acccgttggg gtgcgggtgc
     5041 ccttgccgcc gctggaacag cgtgattgaa aaaccatggt gttgccttca aaggcggtga
     5101 ccatctgtgt gcccaggtct acgtggatga gcttttcaga ttcgggtaca tccggtgaga
     5161 gcagggtcag ttcttcactg gggatcaggc gcaggttgta gtgcggaacg tagaaggatt
     5221 tcttgacata gctatcgtaa atctcgtacc agatgctttt ttcttcacgt gtcacaacga
     5281 cctttttgac ccaatgggtg ctgccataat acagccgcgg tccgttcttt gcatacgtaa
     5341 atggaatcaa tctggtttca ctcatcggca cgacaatttc gccaagctga ccttctgcgg
     5401 ggatatcgta cacgggcttc tgaagtttgg tttccacggg ttgaagccag cctgagtagg
     5461 tatacccgcc atccacttga taccaggcct tattgaaggg attgccttca tcgccttcca
     5521 cttcggcttg cagttcaacc accaggtcaa tgccgtagag gtgtatctgc tttgcttgaa
     5581 aagaaggggc atcgtagagc ggaacgccgc tgtaggtgat gcgtccctgt gaggagggag
     5641 gggcggctaa agcacggtca atgcccattt cggagagagc aagcccgagt gcgccagagg
     5701 cggagagttt caaaaagtcg cggcgggaga ggttaagttt attcatcatg attttttgca
     5761 gacggttttc cgatcagaat cttacaggac gatttccaca tcggtgccta cgccgccgaa
     5821 ggaaaattcc ttggtgaagg tgacctgcgg tgcagaccag cggtaaagcc atttggcagt
     5881 ggatgtggaa aggttgatgc agccatgcga gcgcgggcgg ccaaagtcat tgtgccagaa
     5941 ggtgccgtgg aaggatatgc ccccttcggt gatgtattgc acccagggca caccgggcag
     6001 gtcgaacccg ctggcggcga tatctccggc ggccatgtgg cgggaggggc gtttgtagta
     6061 ggtgataaat gcgccctgcg gcgtggtgta ggtgcccaca cggaagatgc cgcccgtgga
     6121 taccgaggcg acaaagacgg gcaggttgtt ttcgtaggca acgagaatct gctcgtcgag
     6181 ctgaacttgt atctttttgt tttccacctc tggtgagatg ggggcgatct cggcttcgct
     6241 catggggcgc atgtgctcgg agcgggcata atacagcttg tcgaatttat cgtcgcggac
     6301 ctgataccag acttgtccat tttcggcggt gacgacggcc ttgacccagt gggtggtttc
     6361 ataatacatg cggtaggcca ctttggaggt tttatcgggc atttcgtagg catcggtgta
     6421 gggtacgctg atctcggtga gcatgccatc tttgggaatg ttcaactggg gtttgttcag
     6481 cagggtgcgc acgggttgaa tattgcccga atacacgtag ccttccttgc cgatctcgta
     6541 ccatacgcgg ttgtgcgagt tgacatcgtc actgatgacg gcgttggtga tgttgagtag
     6601 tttatcgcgg tggcaaaacg tgacttgtgt ggcctcggtg gaaggtttgt cgtacatcca
     6661 gatcatgcgc gtggtgacac gcccctgcac agcggcaaag ggatcgtcaa aatgaaaatt
     6721 gagcggaggg gtcagcaagc cagcaagacc caatccgccc agtttcagaa aatcacgtcg
     6781 cgataatttg g
//
LOCUS       DAOQLC010000175         6760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4729_length_6760_cov_19.228486, whole genome shotgun sequence.
ACCESSION   DAOQLC010000175 DAOQLC010000000
VERSION     DAOQLC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4729_length_6760_cov_19.228486"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3296)
                     /locus_tag="PL000_19380"
     CDS             complement(<1..3296)
                     /locus_tag="PL000_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91104.1"
                     /translation="MKHIKSFNVMVAMLLVCTALSFQNVTATGVSVFNSIPDPLPGNL
                     PSQPYQAQQAAEFGDHIQLAGTSRNLSSVTVTMSSWARHSEYPNLSEVGFAHPLTLNI
                     YNVDKSGTVPALGALIASVTQEFVMQWRPEGDPTCPDTGYGAGFAWRAVNGTCYNGFA
                     FNIVFDLSEEGIVLPDEIIYGIASNTETWGYDPIGTPGPYNSLNFAVNNISAPSTGID
                     VEPDAVFFNTETAAWYADGGAGGVGIFRRDTGWTGYVPAVRFDVEEAVVTPELTVKVQ
                     DSLGNPVEGAVITYAVGSPNAGWQTFGTTAADGTISRSDLTVGTNYHFYASYNASTST
                     SQALVFDGDDEVVFETVLVKAKVETCLGGAIEGAAVTYGSVNGGFLSFGTTGSDGFVS
                     KELFPGYDRTFYASVNHTSSALQTATVVEQGDPLVTFETTKVTINYSGSVNHGSANGG
                     WYPFTKPAMEMFAGARTFLINGTQVAATVPECDFSTGDLTVKFPGIGSVHTYLKKSDG
                     VNGVANGVQVASLTWKNDEAVFADVPNDVYDIVVVKGAQTKIVDNVIVLGDKTVEGLV
                     STLTVEFPGVGSVHAYAKMPDGIAGVAGGGGVEERTWKNDLAELIVLKGTYDVLVVKG
                     AKNYVVDNVDCTGETCLVNGIVATLTVKFPGIGSVHTYARMPDGGGAVEERTWKNDQS
                     EMVVLKGVYDVSVVKGAKTYIVEDVDCTGNTCLVDGIVATLTVKFPGIGSVHTYAKMP
                     DGAAGVAGGGGVEERTWKNDQSEMVVLKGTYDIQVVKGAKTYVVDNVDCTGTSCQVDG
                     IVATLTVKFPGIGSVHTYAKMPDGVANTAGGGGVEERTWKNDQSEMVVLKGMYDVSVV
                     KVAKTYVADDVDCSGDTCLVEGIVATLTIKYPGMTGIHNYVKMPDAISNSATGGSVEE
                     RTWQNNESVMTVLRGFYDVLIVKGSASFIYDDVDCTALTCTLDKASLTVKFPGITSVH
                     TYVRKSDNVAGTATGAQVASQTYKADQAVFADLPNGFYDVIVVKGAQTKVIDNVTVLG
                     GWATVDKIVATLTVEFPGIAGVHTYAKVNDGVVGTAAGGSVEDRTYKNDEASMVVLKS
                     TYDVIVVKGA"
     regulatory      complement(3410..3520)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(3765..4469)
                     /gene="cysE"
                     /locus_tag="PL000_19385"
     CDS             complement(3765..4469)
                     /gene="cysE"
                     /locus_tag="PL000_19385"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017638459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HNC91105.1"
                     /translation="MLKTIREDVQSVLDRDPAARSVWEILLCYPGVYAIWIHRVAHRL
                     WKNGFKLIARWLSQLSRSLTGIEIHPGATIGRNFFIDHGMGVVIGETAEIGENVTLYH
                     GVTLGGTSLQKTKRHPTIEDHVVIGAGAKVLGAITIGAHSRIGANAVVVKSSPPNSVI
                     IGVPGQIIVRSQDKAINNKPDLEHGLLPDTIGETLAALVSHVERLEKRLDGHTSVSPA
                     LHTPDHGIWHGEDFAI"
     gene            complement(4537..5475)
                     /gene="cysK"
                     /locus_tag="PL000_19390"
     CDS             complement(4537..5475)
                     /gene="cysK"
                     /locus_tag="PL000_19390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNC91106.1"
                     /translation="MKIANNVTDLIGNTPLVRLNRISDGTKAEVVAKLEFYNPAHSVK
                     DRIGAAMIDAAERDGLLTKDTTIVEPTSGNTGIALAMVAAARGYKIILTMPETMSKER
                     RMLLRAYGAELVLTPGPDGMGGAIRKAEEIAGSDPKKYFLPQQFKNLANPEIHRKTTA
                     EEIWRDTDGKIDFLVSGIGTGGTITGTGEVLKSRKPSLKAIAVEPDASPVLSGGAKGP
                     HPIQGIGAGFVPDILNTKIYDEIIRVKNDDAFQTARRMAREEGLLVGISSGAATWAAL
                     QVANRAENAGKLIVVIIPSFGERYLSTALFADLADG"
     gene            complement(5861..>6760)
                     /locus_tag="PL000_19395"
     CDS             complement(5861..>6760)
                     /locus_tag="PL000_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91107.1"
                     /translation="NLSDDPVLAGEQIQCQRKVEEPKHLAIPAFELMDGQYPVDAAYE
                     QGCFPSIPSELRKNLMTESRDVYKSHTFRTFQQFTLGKKMFEETYQLPADAKREARSL
                     LLTEDKANISAEIRERLRHERNSIAGFTARPSRPPREWDGSHRGYAPEAELGLELAGL
                     ADIPLMSFGKLEFIAAQHGLNPATLMKPSPFHALAGIIAAWTGNELSALNAVYEWHTS
                     GKLNGKFNELPKSFELYVIEDTMGGVRATRAAGEVLREAGFGVSVHALGLTSGIEQKA
                     EAFRKADVPFFENWEDLIAFGNL"
BASE COUNT         1747 a         1899 c         1440 g         1674 t
ORIGIN      
        1 ttcgcgccct tgaccacaat gacgtcatag gtgctcttca ataccaccat ggatgcttcg
       61 tcattcttgt aggtgcggtc ttcaacactt ccgcctgcgg ctgtgccaac cacaccatca
      121 ttcaccttgg cataggtatg gacacccgca ataccaggga attcaacggt caacgtggcc
      181 acgatcttat ccacggtggc ccaaccgcca agcacggtca cgttatcgat gaccttggtc
      241 tgtgcgccct tcaccacgat cacatcataa aaaccgttcg gcagatcggc aaagacagcc
      301 tgatcggctt tataggtttg agatgcaacc tgtgctccgg tcgccgttcc cgcaacatta
      361 tcgctcttac gcacataagt atgtacgctg gtaattccgg ggaacttcac cgtgaggctg
      421 gctttatcca atgtgcaggt cagggccgtg caatccacat cgtcataaat gaacgaggcg
      481 ctgcccttca cgatcagcac atcatagaaa ccacgcaaaa cggtcatgac agattcattg
      541 ttctgccagg tgcgttcttc cacagatccg cccgttgccg aattgctgat cgcatcgggc
      601 atcttgacgt agttatggat tcccgtcatg ccggggtatt tgatggtcaa tgtggcaacg
      661 attccctcca caaggcaggt atcgccgctg cagtcaacat catcagcaac ataggtcttc
      721 gcaaccttga ccacggaaac atcatacatg cccttgagca cgaccatctc agactggtca
      781 ttcttccagg tgcgctcttc cacaccgccg ccgccagcag tattagcgac gccgtcgggc
      841 atctttgcat aagtgtggac actgccaata ccagggaatt tcacggtcaa tgtggcgacg
      901 atcccatcca cctgacaact ggtgccggta caatctacat tatccaccac gtaggtcttc
      961 gcgcctttaa ctacctgtat atcgtaggtg cccttgagca cgaccatctc agactggtca
     1021 ttcttccagg tgcgctcttc cacaccgcca ccaccagcca cgcccgcagc gccatcaggc
     1081 atctttgcat aggtatggac actgccaatg ccggggaatt tcacggtcaa tgtggcgaca
     1141 atcccatcca ccaggcaggt attgccggta caatccacat cttcgacaat ataggtcttt
     1201 gctcccttta ctacagaaac atcatacaca cccttgagga caaccatttc agattggtcg
     1261 ttcttccaag tgcgttcttc cacggctcca ccgccatcgg gcattctggc ataggtatgg
     1321 acactgccaa tgccggggaa tttcaccgtc agcgtggcaa caattccatt cacaaggcag
     1381 gtttcgccgg tgcaatctac attgtccact acatagtttt ttgcaccttt gaccaccaac
     1441 acatcatagg tgcctttgag aacgatcagt tccgccaggt cgttcttcca ggtgcgctct
     1501 tccacaccgc cgcctccagc cacacccgca ataccatcag gcatctttgc ataggcatga
     1561 acactgccaa cgccggggaa ttcaacggtc aaagtggaga ccaaaccttc cacagtttta
     1621 tcaccgagca caataacgtt atcaacgatt ttggtctgtg cgccctttac caccacaatg
     1681 tcatacacat cgttgggcac atcggcgaag accgcttcgt cattcttcca tgtgagggaa
     1741 gcaacctgca caccattcgc aacaccattg accccatcac tcttcttcaa gtaagtgtga
     1801 acgctgccaa tccctgggaa cttaacggtg agatcacccg tggagaaatc acattcaggt
     1861 acagttgcag caacctgcgt accgttgata aggaaggtgc gcgcaccggc aaacatttcc
     1921 atggctggct tggtaaatgg ataccagccg ccgttggcgc tgccatgatt gaccgaaccg
     1981 ctgtagttaa tcgtcacctt tgtggtttcg aatgtaacca acggatcacc ttgttcaaca
     2041 actgttgcgg tctgcaatgc cgaagaagtg tgattcaccg atgcatagaa cgtgcggtca
     2101 tagccaggga acagttcctt cgaaacaaaa ccatcactac cggttgtacc aaaggatagg
     2161 aaaccgccat taacactacc ataagtgacc gccgcgcctt caattgcgcc acccagacag
     2221 gtttccactt ttgccttgac cagaacggtt tcaaagacaa cctcatcatc gccatcgaaa
     2281 accaacgctt gcgaagtgga tgttgaggcg ttgtaactgg catagaagtg gtagttcgtg
     2341 ccaacggtca ggtctgaacg gctgatcgtg ccatccgcag cggttgtacc aaatgtctgc
     2401 caaccggcat tagggctgcc aaccgcgtag gtaataacag caccttctac tgggttaccc
     2461 agactgtcct gcactttgac cgtcaattca ggcgtaacaa ctgcttcctc aacatcaaag
     2521 cgaactgcag gaacataccc ggtccaacca gtgtcacggc ggaaaatacc aactccaccc
     2581 gcaccaccgt ctgcatacca tgccgctgtt tcggtattga agaaaactgc gtctggttcc
     2641 acatcgatac ctgtacttgg cgctgaaata ttatttaccg caaaattcag cgagttgtaa
     2701 gggccaggag taccaatcgg gtcatatccc caagtttccg tgttcgatgc gataccgtaa
     2761 atgatctcat caggaagaac aataccttct tcgctcaaat caaatacgat attaaaggcg
     2821 aagccattgt agcaagttcc attgaccgct ctccaggcaa agcctgcccc atatccagtg
     2881 tctggacagg tggggtcacc ctcaggccgc cattgcataa caaattcctg agtcacactt
     2941 gcgatcaacg caccaagcgc tggaactgtt ccgctcttat ctacattgta aatgttaagt
     3001 gtaagtggat gagcaaatcc cacctcagac aggtttggat actccgaatg ccgtgcccaa
     3061 ctgctcatgg tcaccgtcac actggaaaga tttcgagatg tcccagccaa ttgaatatga
     3121 tccccaaact ctgccgcttg ttgggcctga tatggctggc tgggtaaatt cccaggcaat
     3181 ggatcgggaa tgctgttaaa aacagatacg cctgtggcgg ttacattctg aaacgataga
     3241 gcggtgcata caagcaacat cgcaaccatc acgttaaaag acttgatatg tttcattttt
     3301 tctccctttc aaaaacatgg actgcacgaa caaaaatgaa gtgccattta tgtcatcctg
     3361 cgttccttta taaaacacct cctttttccg gtttaaatta aaaaccggcc atatgggccg
     3421 gtttcgctat tagctcccca aagacctatc tgttggcatg gtaagtgcca caagataagc
     3481 gaccaaataa agtcattgga tctttgcttc cagaaaaata ttcaattttc atctcatgtc
     3541 taaaggaaat tactgcttca tcacaaaaca aatataagac ttccatccct gtcataactt
     3601 accaataggt ttgtttctca atgggttact tctaaaaatc ccacagaaga tgttgcagta
     3661 gaatcagtat acaagtttta tttaacaatt acaagtgttt cttattatct ctttcctaat
     3721 tggacaaatt gggcttactt catccagcga cacatcttta tggattaaat tgcaaagtct
     3781 tcaccatgcc aaataccatg atcgggggta tgtaaggcag gtgaaacgga tgtatgaccg
     3841 tccaatcttt tttcgaggcg ttccacatga gaaacaaggg cagctaacgt ctcaccgatc
     3901 gtatcgggca agaggccatg ctctaggtct ggcttgttat tgattgcctt atcctgacta
     3961 cgaacaatga tctgccccgg cacaccgatg atgactgagt tcggcggaga tgatttcact
     4021 acgactgcat ttgcgccgat acggctatgc gcgccaattg taatagcccc caataccttc
     4081 gcgcccgcac caatcacaac atgatcttcg atggttggat gcctcttggt cttttgcaag
     4141 ctggtgccgc ccaatgtaac cccatgatac aaggtgacat tctcaccgat ctccgcagtc
     4201 tcaccaatga caacccccat cccgtggtca atgaaaaagt tgcggccaat ggtggccccg
     4261 ggatggatct ctattcccgt caaactgcgc gagagttgtg aaagccagcg tgcgatcaat
     4321 ttaaatccat ttttccatag ccggtgagcc acgcggtgaa tccaaatggc atatacaccg
     4381 gggtaacaca aaaggatctc ccacacgcta cgagcggcag gatcacgatc caatacagat
     4441 tgaacatctt ctcgaatggt cttcaacata tttactcact ccatgcagag atgcggcagg
     4501 cctgccgcat ctctgcatgt gaattaaaaa aaacagttat ccgtcagcta ggtcagcaaa
     4561 caaagccgtg gacaaataac gttcaccaaa tgaagggatg atcaccacga tcaacttgcc
     4621 agcattttca gcacgatttg ccacctgcaa ggcagcccaa gtggcggcac cggaagaaat
     4681 accaaccaac aatccttctt cacgcgccat gcggcgggcg gtctggaagg catcatcatt
     4741 ttttacacgg atgatctcat catagatctt cgtgttgagg atatctggaa caaagcccgc
     4801 accaattccc tgaatgggat gcggtccttt tgcaccacca gacaaaacag gagaagcatc
     4861 gggttctaca gcaattgctt tcaaagatgg tttgcgcgat ttaagaacct cacctgtacc
     4921 ggtgatcgtt ccaccagtgc caatccctga aaccagaaaa tctatcttcc catcggtatc
     4981 tcgccaaatc tcttcggctg tcgttttacg atggatttca ggattcgcca ggtttttgaa
     5041 ctgttgcgga agaaaatatt ttttcgggtc ggaacctgcg atttcttcgg ctttgcgaat
     5101 cgcgcccccc attccatctg gaccaggtgt caaaaccaat tctgcgccat aagctcgcaa
     5161 caacatgcgg cgttccttac tcatcgtttc aggcatggtc aggatgattt tatatccacg
     5221 tgcagcagca accatagcca gagcgatacc ggtattgccg ctggtaggct ccacaatggt
     5281 cgtatctttt gtgagcaaac catctcgttc cgccgcatcg atcatggcag caccaattcg
     5341 gtcttttaca gaatgagccg ggttgtaaaa ttccagtttg gcaaccacct cggcttttgt
     5401 tccatcagaa atacgattca gacgaaccaa aggtgtattt ccgatcagat ctgtaacatt
     5461 gtttgcgatt ttcatattgc tctccttata atgatattgg ttttaaataa ataaaacggc
     5521 tgtctgccaa ccggaataaa tcccggtcta cgcaaacgat gggcgcaatt cacaagccgt
     5581 gccagcggct tggagaagtt gcgggatcac atccaccgtt tacggcagtc agccgtttgt
     5641 atctctgtaa agaggcggtg gttagcgacc ccgccaggta cacatgcagt tcaaaaattc
     5701 tattggtttc atcattgcct ttagaaaagg cgattcgcct ttttcttacc tcgtaaattt
     5761 gtcatacaga tattttgagc cgcttcaaag ccttgaaggt tacgcattat aaccatacaa
     5821 gaactttttt cgaaggttaa tcacaaatct ttcgacggct ctataaatta ccgaacgcga
     5881 tcaaatcttc ccaattttca aaaaatggca catccgcttt gcggaatgcc tctgccttct
     5941 gctcgatgcc cgaagtcaag cccagcgcat gtacactcac cccaaaacct gcctctcgca
     6001 agacctcccc cgccgcccgt gtcgcacgca caccgcccat cgtatcttcg ataacgtaca
     6061 actcaaacga ctttggcagc tcgttaaatt tcccattcaa cttacccgac gtatgccatt
     6121 catacaccgc attcaatgcc gacaattcat tccccgtcca tgccgcaatg attccagcca
     6181 gcgcgtggaa cggcgatggc ttcatcaacg tcgctgggtt caagccgtgt tgcgccgcga
     6241 taaattccaa ctttccaaag gacatcaacg gaatatccgc cagccctgcc aactccaagc
     6301 ccaactccgc ttcaggcgcg tacccacgat gtgaaccatc ccattcccgt ggaggcctgg
     6361 aaggtcgggc ggtaaagcct gcgatggaat tccgctcgtg ccttaacctc tcgcggattt
     6421 ccgcactgat gttagcttta tcctccgtca gcaaaaggga ccgcgcttca cgcttcgcgt
     6481 ccgccggtag ctgatacgtt tcctcgaaca ttttcttccc caaagtaaat tgctgaaacg
     6541 tgcgaaacgt atgcgacttg tacacatccc ttgattcagt catcaaattc ttacgcaact
     6601 cactcggtat ggatggaaaa catccctgct catacgccgc atccaccgga tactgcccat
     6661 ccatcaactc aaacgcggga atagctaaat gcttcggctc ctctacctta cgttgacatt
     6721 gaatctgctc cccagcaagg acaggatcat ccgataaatt
//
LOCUS       DAOQLC010000176         6713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4767_length_6713_cov_17.107690, whole genome shotgun sequence.
ACCESSION   DAOQLC010000176 DAOQLC010000000
VERSION     DAOQLC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_6713_cov_17.107690"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PL000_19400"
     CDS             complement(<1..276)
                     /locus_tag="PL000_19400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91108.1"
                     /translation="MSDEWTKGPRVFYVRQEDFSNRALIRELGEKWPDDVKGEPSSAW
                     VHVIEKSAYDKLERAWEVESEAHTFYKREWEAACERYDSLKADYDELK"
     gene            complement(293..493)
                     /locus_tag="PL000_19405"
     CDS             complement(293..493)
                     /locus_tag="PL000_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91109.1"
                     /translation="MTWEWDKERDEAADEYVGKPADDAYLASQRGFKDGADWGRAYEL
                     KRAAELVEKLKAIIWAYENNVG"
     gene            complement(498..986)
                     /locus_tag="PL000_19410"
     CDS             complement(498..986)
                     /locus_tag="PL000_19410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNC91110.1"
                     /translation="MLETLQWGRKVKYPERYRAPHPGGIPSITFTASELIELATLKNQ
                     DQYTRGNNMEQITVNGKTYYSEIELRFHNRTRKATSGCHEWIGSKLKSGYGRMEYNGH
                     RLLAHRLSYMVQIGSIDPGMEIHHKCGNKSCVNPDHLSQVTPSENNRQYQNTRYGNGY
                     GW"
     gene            complement(953..1420)
                     /locus_tag="PL000_19415"
     CDS             complement(953..1420)
                     /locus_tag="PL000_19415"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC91111.1"
                     /translation="MNYRTISPDELKDILEKHKTWLSDPSKGAKANLRSADLRSADLS
                     SADLRSADLSSADLRSANLSYADLRSADLSYADLSSANLRSADLLIFKFQRHTVYYTF
                     DGYLRIGCHALPITEWSLRYEEIGRKEGYTDKQTKLYGDFIKMCLRHFNGAEK"
     gene            complement(1417..1650)
                     /locus_tag="PL000_19420"
     CDS             complement(1417..1650)
                     /locus_tag="PL000_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91112.1"
                     /translation="MAIDRTLPDRLPDQYSYRVEMQVRSPFMVGYHVELDELFLFYTD
                     QNDLTWVFREGRLDAIGFWEFFNFNKAIILGEL"
     gene            complement(1650..1898)
                     /locus_tag="PL000_19425"
     CDS             complement(1650..1898)
                     /locus_tag="PL000_19425"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC91113.1"
                     /translation="MTTTLGLLIRAARQAKGLTQTELAKKLGYSTPQYISNVECGKCS
                     LASKKVIRTSKLLGISLKTMRRLMIKDYEQKLIRKGLR"
     gene            complement(1912..2808)
                     /gene="bet"
                     /locus_tag="PL000_19430"
     CDS             complement(1912..2808)
                     /gene="bet"
                     /locus_tag="PL000_19430"
                     /inference="COORDINATES: protein motif:HMM:NF015776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HNC91114.1"
                     /translation="MSDLVKSDSATFSESEIATIKQTIAPNLTNEELSLFVSTCKRTG
                     LDPFSRQIYVTASEWTDKRGQVQRKISISATVDGFRVIAERSGKYAGQVGPLWCGNDG
                     VWVDVWLKRDPPQAAKVGVLRQDFKEPIWAVALFESYRQDRSPVWNKMPEQMLAKCAE
                     SLALRKAFPNDLSGIYSQEEMAQAENSSGIVRDAQPKALLGDSQRVIGSDGEILAPDF
                     SNEPWPDGDSAERHESNPEDLGEYIIPFGKTMKGKRLKDIHHDSLRKSVAWVRNEANK
                     PLSRSLSDFLEHAEAYLKEQSQ"
     gene            complement(2844..3131)
                     /locus_tag="PL000_19435"
     CDS             complement(2844..3131)
                     /locus_tag="PL000_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91115.1"
                     /translation="MGEFEGVDEVVLIVKQLRKYIGECRARKVGIVIGQGELSALLTD
                     HERLTKMLEELLFTMSGVMQITDNIFSRSKIFASAIRCDQIAQGLEVDQHD"
     gene            complement(3574..3915)
                     /locus_tag="PL000_19440"
     CDS             complement(3574..3915)
                     /locus_tag="PL000_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HNC91116.1"
                     /translation="MNCKHEHTIEREVIFKNNTKHIESRCEDCGKHVKYMPQPIDLSP
                     EEIPEHVIIWGKHKGKRLCEIPPDYLGWMVREKVGRDARIAAKYLEMVRNASQASSMS
                     QELAHELRIDR"
     gene            complement(3912..4907)
                     /locus_tag="PL000_19445"
     CDS             complement(3912..4907)
                     /locus_tag="PL000_19445"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNC91117.1"
                     /translation="MSDEINYKELFNRAKSCFDDAPKVFQDNEHRGRNLIPFGISFLD
                     GALDGILPNDLILIGAATGAGKTTLATQLAKQVATSGKRVLYFALEADKREIAARLIF
                     TSLSRQFFNDQFRSDEKISFKNFYLGKLDKCFSRYSFEKEEWLKNIRCIYSETGYGLE
                     DFRRDLTQLRSEIDVVIIDHLHYLDLPDESTESYKQAVKEIRNLALEYSKPVILVAQL
                     RKKSLAQPSLCPENDDFHGSSDITKIATKVITIAPGGNYIPNASSTKISTLMRAGKYR
                     LDGSVVHFIASMNYDIPTGAYSSKFTLGRQAVVKNKIQFEPIENEMLPDWAKGAR"
     gene            complement(4900..5787)
                     /locus_tag="PL000_19450"
     CDS             complement(4900..5787)
                     /locus_tag="PL000_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91118.1"
                     /translation="MNQDIMADRHLYRIHDPGVEKGCFPVADISEALSWNEKQWGIFW
                     TVNKFKNPGERVAASLDRILCWAVDLDTGTKAEQLQTIAKFAEPSALIETKNGHHVYY
                     DAIDPTTKAFPEIMGRLVENLNADPNAKDLSRILRVPAFCHWKDPKNPFAIKLVHESN
                     RVYTEKDMLILFPELKKKTNDEVEMQTLKRTLSFQKDDTLFDKIYGLDCEAALMRLSG
                     TIAVDYETFSFRRVSRGNVNILVNNKSTSCFIDPNKRIGGGGGTAGPTIWQWVNYYHR
                     DHQKTYGYIKTYLPELFCE"
     gene            complement(5784..6530)
                     /locus_tag="PL000_19455"
     CDS             complement(5784..6530)
                     /locus_tag="PL000_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91119.1"
                     /translation="MAKRLTESEKWKDPWFRQLSPKMKLVWLYILDQCDHCGIWEPDI
                     GLMSLQIGFDITNDEIDALVNDHKIELFGKKYVISAFLSFQYGAKQNNSKILSNCLER
                     LDKLKQQVTLTQGLPNPYLRDKVKVKVKVKVKVPDDEIDGVARQKVEREVFDFEAIYE
                     NYPRKIGKSSGLKSCAKQIKTKEEYEQLLLAVKNYSAQCLLDQTEKKFIKKFNNFLKD
                     DYWRDFIDADIPTPELSYEERMRKIAERNV"
BASE COUNT         1610 a         1658 c         1406 g         2039 t
ORIGIN      
        1 tttcagctca tcataatccg ctttcaaaga gtcatatctc tcacatgctg cttcccactc
       61 acgcttataa aatgtgtgcg cctcagattc tacctcccat gcacgctcaa gcttgtcata
      121 ggccgatttc tcaatgacgt gaacccatgc agaacttggt tcgcctttta catcatctgg
      181 ccatttctca cctagctctc taatcaaagc acgattagag aaatcctctt gtctaacgta
      241 aaacacgcgc ggccctttag tccattcgtc gctcatctcg ttctctttgt ctttacccaa
      301 cattattttc ataagcccaa ataatagctt ttaatttctc aaccaattca gccgcgcgct
      361 ttagttcata ggccctgccc caatcagccc catctttaaa acctctttgc gacgccagat
      421 aggcatcatc agcaggtttg ccaacatatt cgtcagccgc ctcatcgcgt tctttatccc
      481 attcccaggt cattaaatta ccacccgtag ccgttgccgt agcgagtatt ctgatattgg
      541 cgattgttct ctgaaggagt tacttggcta agatggtcag gattaacaca ggatttattt
      601 ccacatttat gatgaatctc catacctgga tctatagatc caatttgaac catatacgaa
      661 agtcgatgcg ccaaaagcct atggccatta tactccatgc gcccgtaacc ggattttaat
      721 ttactcccaa tccattcatg acatccagat gttgcttttc tagttcggtt atgaaatcgc
      781 aattcaattt ctgaataata cgtttttccg ttaactgtta tttgctccat gttattccct
      841 ctcgtatatt gatcttggtt ttttagtgtc gctaattcaa tcagctccga tgctgtgaag
      901 gttatagaag ggataccgcc tgggtgaggt gcgcggtatc gctcggggta tttcactttt
      961 ctgccccatt gaagtgtctc aagcacattt tgataaaatc accataaagc tttgtttgtt
     1021 tgtctgtgta gccctctttt ctaccaatct cttcgtatct caatgaccat tcagtaatgg
     1081 gcagtgcgtg acagccgata cgaagatagc catcaaaagt atagtataca gtatgtcgtt
     1141 gaaatttaaa aattagcagg tccgcagagc gcaggttcgc agagcttagg tctgcatagc
     1201 tcaggtccgc agagcgcagg tccgcatagc tcaggttcgc agagcgcagg tccgcagagc
     1261 ttaggtccgc agagcgcagg tccgcagagc ttaggtccgc agagcgcagg tccgcagagc
     1321 gcaggttcgc ctttgcgcct ttgcttgggt cgctcagcca agtcttatgc ttttccaata
     1381 tatcttttaa ttcatctggg gagattgtcc ggtagttcat aattctccca atataattgc
     1441 tttattaaaa ttaaaaaact cccaaaaccc tatagcgtct agtcggcctt cgcgaaacac
     1501 ccaggttaaa tcattctgat cggtgtaaaa caaaaacaat tcatctagct caacatgata
     1561 gccaaccata aacggtgagc gcacctgcat ttcgacccga tagctatact gatccggcag
     1621 cctatcgggc aaagttcgat cgattgccat tatcgtaggc cttttctaat caatttttgt
     1681 tcatagtcct tgatcattaa ccgtctcatg gttttaagac taatccccaa aagcttagat
     1741 gtgcggatga ccttctttga tgccagcgag catttgccac actctacgtt tgaaatgtat
     1801 tgcggcgtcg agtagccgag cttctttgca agttcagttt gagtaagtcc tttggcttgt
     1861 cttgcggccc tgattaatag gcctagggtc gtagtcattt gatctccttt attattggga
     1921 ttgttcttta agataggcct cggcatgctc gagaaaatcg ctaaggcttc gcgagagtgg
     1981 tttgttggct tcattcctaa cccacgccac tgattttcga agcgagtcgt ggtggatatc
     2041 tttaaggcgc ttgcccttca tcgttttgcc aaaaggaatg atgtactcgc cgaggtcttc
     2101 tgggttactt tcatggcgct ctgcgctatc gccatcgggc caaggttcgt ttgaaaagtc
     2161 tggggccaat atttctccat cggacccaat aactcgctgc gaatcgccta atagagcttt
     2221 tggttgggcg tcacgcacaa ttcccgaact gttttcggcc tgagccatct cttcctgtga
     2281 ataaattcca ctgaggtcgt tggggaatgc tttacgcagc gcaagagatt ctgcgcactt
     2341 tgcaagcatc tgctccggca ttttgttcca aaccggggag cggtcttgcc ggtaagattc
     2401 aaaaagcgcc acggcccaaa taggctcttt gaagtcctga cgaagcacgc ccactttcgc
     2461 agcctgtggc ggatcgcgct taagccaaac gtcaacccaa acgccatcgt tgccgcacca
     2521 aagcgggcca acctgacctg cgtatttgcc actgcgctca gcaattacgc gaaacccatc
     2581 gaccgtggcc gaaattgaaa tcttgcgctg tacctgcccg cgcttatccg tccactccga
     2641 tgcggtcacg taaatttggc gcgagaaagg atcaaggccc gtacgcttac aagtcgaaac
     2701 aaaaagcgac agctcttcgt tcgtaaggtt tggcgcaatc gtttgcttga tggttgcaat
     2761 ttcagattct gaaaacgtag ccgaatcgct ttttaccaag tcactcatca catcacctct
     2821 tttgattgtt actagtattt tgtttaatcg tgttggtcaa cctctaagcc ctgcgcgatc
     2881 tggtcacagc gaatagccga cgcaaagatc ttagatcgtg aaaagatatt gtccgtgatc
     2941 tgcatcacgc cgctcatcgt gaacaatagc tcttcaagca ttttcgtcag gcgctcgtga
     3001 tccgtgagca gcgctgacaa ttccccttgg ccaatgacta tccctacttt tctagctcta
     3061 cattccccaa tgtattttct gagctgcttg acgatgagca cgacttcgtc aactccttcg
     3121 aactctccca ctcaccctcc tcggtttgct taatcactgc aatcaaacta tccgaactct
     3181 ttgcgatcat ggaaagtgtg gcaagcgcca cgtcgatctt taaccttaac cgcttgttct
     3241 caatttctaa ctctctacgt gttcgcatta ctaatccgtt catagacgtc gtagttgtaa
     3301 taaatcgggt ctctcgtatt ctcgccgccc cagttctccc cattcggccc atagcttcga
     3361 ttgttctggt aaccccaaag ctttcttatg gtgcctatgt agcgccattc gttctctggc
     3421 gttggcggag ggctcccctc agaaaccatt ttcttattaa cgagccaatc gtgttcgtct
     3481 tgccaattat taaacttgaa ttctagccag cccattcatc acctctttgg gcgtagatat
     3541 tattaatttg tgtaatagta aactaaaaag tgattagcga tcaattctta gttcatgcgc
     3601 aagctcctgg ctcatgctgg atgcctgcga agcattgcgc accatctcaa ggtatttagc
     3661 tgcaatccga gcgtcacgcc ccaccttctc gcgaaccatc caaccaaggt agtctggcgg
     3721 gatctcacat agtcttttgc ccttgtgctt tccccagata attacatgct ctggaatttc
     3781 ctcagggctt aggtcgattg gttgcggcat gtacttaacg tgcttaccac agtcttcgca
     3841 tcggctctcg atgtgcttcg tattgttttt aaatattacc tcgcgctcga tcgtgtgttc
     3901 atgcttgcag ttcatcttgc tcccttcgcc caatcgggca gcatctcgtt ttcaataggt
     3961 tcaaactgaa ttttgttttt gactacagct tgtctgccaa gtgtgaactt acttgagtat
     4021 gctccagtag gaatgtcgta gttcatcgaa gcaataaagt gcacaacgct accgtcgagg
     4081 cgatatttgc cagcgcgcat aagtgtagaa attttcgtag atgatgcgtt tgggatataa
     4141 ttaccacccg gcgcgattgt tatcaccttg gtcgcgatct tagtgatgtc gcttgatcca
     4201 tgaaagtcat cgttctctgg gcagagcgaa ggttgagcca aagatttctt tctcaactgt
     4261 gccacaagaa ttacaggctt tgaatattca agggctaggt ttctgatttc ttttaccgcc
     4321 tgcttatacg attctgtcga ttcatctggc aggtccaagt agtgaaggtg atcgatgatc
     4381 acaacgtcga tttcacttct aagctgagtt aagtctctcc gaaagtcttc aagtccgtac
     4441 ccggtttcgg aatagatgca tcgaatattt tttagccact cctctttttc aaaggagtac
     4501 cttgagaaac acttatcaag cttgcctaaa taaaagtttt tgaatgatat tttttcgtcc
     4561 gacctgaact ggtcattaaa gaactggcgc gagagtgatg taaaaattaa acgtgctgca
     4621 atctcacgct tatcagcttc aagtgcaaaa tataaaaccc gcttccccga tgtggccacc
     4681 tgctttgcga gttgggttgc gagagtcgtc ttgccggccc cagttgcagc gccaatgaga
     4741 attaaatcgt tgggtaaaat tccatctagc gcgccatcca gaaatgaaat cccgaacggg
     4801 attaaattcc tgccacgatg ttcgttgtct tgaaatactt ttggcgcatc gtcgaaacaa
     4861 ctctttgcac gattaaataa ttctttgtag ttaatctcat cactcacaaa acaactcagg
     4921 taaatatgtt ttaatgtagc cgtaagtctt ctggtggtct cggtggtagt aattgaccca
     4981 ctgccaaatc gtaggtccag ccgtaccgcc accgccgcca atgcgtttat tggggtcaat
     5041 aaagcatgaa gtagatttat tgtttactaa aatgtttaca ttgccgcgac taacgcgcct
     5101 aaacgaaaac gtttcatagt ccacagctat tgtgccgctg agccgcatga gtgccgcttc
     5161 gcaatcaagc ccgtagatct tgtcgaagag agtgtcatcc ttttggaacg agagtgttcg
     5221 tttaagtgtt tgcatttcta cttcgtcgtt ggtctttttt tttaactcag gaaataaaat
     5281 caacatgtct ttctctgtgt aaacacgatt gctttcatga acgagcttta ttgcaaatgg
     5341 gttctttggg tccttccaat ggcagaaagc cggcacgcgc aagatgcgac ttaagtcttt
     5401 tgcatttgga tctgcgttta gattctcaac aaggcggccc atgatttctg ggaaagcctt
     5461 cgtcgtcgga tcaattgcgt cgtaataaac atggtgaccg ttcttggttt caatgagagc
     5521 cgatggctct gcaaacttgg cgatagtctg aagttgctct gcttttgtgc cggtgtccaa
     5581 atcaactgcc caacataaaa tgcgatcaag cgacgcggcc actcgctcgc caggattctt
     5641 gaatttattc acggtccaga atattcccca ctgcttctcg ttccacgaca gggcctcaga
     5701 aatgtcggcc acggggaagc atcccttttc aactccaggg tcatgaatcc tgtacaggtg
     5761 acgatcggcc attatgtctt ggttcatacg ttgcgctccg ctatctttcg cattctctct
     5821 tcgtaactta gttcaggtgt tggaatatcg gcgtcaatga aatcgcgcca ataatcatcc
     5881 ttaagaaaat tattaaactt cttaatgaat tttttctctg tctgatccaa cagacattgc
     5941 gctgaatagt ttttcacagc caatagcagc tgctcgtatt cttctttcgt tttaatttgc
     6001 ttcgcgcaag atttgaggcc tgagcttttg ccaatcttgc gtggatagtt ttcgtagatc
     6061 gcttcaaaat cgaagacctc tcgctctacc ttttgccgcg ccacgccatc aatttcgtcg
     6121 tcgggtacct ttacctttac ctttaccttt acctttacct tatcccttag gtaagggtta
     6181 ggtaaccctt gggtaagggt tacttgctgt tttaatttat ccaatctttc taaacagttg
     6241 gacaaaatct tagagttgtt ttgtttagct ccatattgga atgataaaaa cgcagaaatt
     6301 acgtactttt taccgaataa ttcgattttg tggtcattga ccagcgcgtc aatttcatca
     6361 ttggttatgt cgaaaccgat ctgcaaagac atcaaaccga tgtcgggttc ccagatgccg
     6421 cagtggtcac attggtccaa aatgtatagc caaaccaact tcatttttgg tgagagttgc
     6481 cggaaccaag ggtctttcca tttttcggat tcagtgagtc gcttcgccac gtcccctcca
     6541 cgaagtgaaa attcgttaac ctttgcgact tgaatatgga ggggttttgt tttaaaactt
     6601 gatccatatt cttgtgtcgc aactcggata taccccatga atctgaagaa gtgaagtggg
     6661 gtttcgttat gcctcaaggc accatctaac taattaaatt ttatgtttga agt
//
LOCUS       DAOQLC010000177         6659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4815_length_6659_cov_23.742429, whole genome shotgun sequence.
ACCESSION   DAOQLC010000177 DAOQLC010000000
VERSION     DAOQLC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4815_length_6659_cov_23.742429"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..881
                     /locus_tag="PL000_19460"
                     /pseudo
     CDS             312..881
                     /locus_tag="PL000_19460"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
     gene            1874..2284
                     /locus_tag="PL000_19465"
     CDS             1874..2284
                     /locus_tag="PL000_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91120.1"
                     /translation="MERPLFIYINSFLLICILGILNNVISYTEELSLSLIYMSLFIIF
                     SEGSKLMIKNIIVSNTFMLMLCYEQLIYLKFKLLIKNLNLSRLILEKKYLKNLFNNLD
                     FEIKDKKNNFLDIKKNINKLKNLNQLLVFILNIK"
     gene            2404..2529
                     /gene="ndhC"
                     /locus_tag="PL000_19470"
     CDS             2404..2529
                     /gene="ndhC"
                     /locus_tag="PL000_19470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HNC91121.1"
                     /translation="MSSYECGFQPFEDTRSRFDVKFYLVAILFIIFDLEIAYLFP"
     gene            2782..3300
                     /locus_tag="PL000_19475"
                     /pseudo
     CDS             2782..3300
                     /locus_tag="PL000_19475"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
     gene            3318..3707
                     /locus_tag="PL000_19480"
     CDS             3318..3707
                     /locus_tag="PL000_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91122.1"
                     /translation="MLNKIKHFYMNFGPQHPAAHGVLRLVLELQGETIIRADPHIGLL
                     HRGTEKLIEYRNYIQGLPYFDRLDYVSMMAQEHSYSLAIESLLKCVVPKRGQLIRILF
                     SEITRILNHLLALTTHALDVGAMTPFL"
     gene            3723..4514
                     /locus_tag="PL000_19485"
                     /pseudo
     CDS             3723..4514
                     /locus_tag="PL000_19485"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5583..>6659
                     /locus_tag="PL000_19490"
     rRNA            5583..>6659
                     /locus_tag="PL000_19490"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2629 a          506 c          953 g         2571 t
ORIGIN      
        1 atttattatt attttatatt tattttgaag ctgttttaat accaatgttt ttaataatat
       61 taatatgagg ttcaagaaaa agaaaaatac atgcttcttt tttatttttt ttttttacat
      121 taattggttc tttatttatg cttttaggta tattatgatt atatttatat gtaggagaat
      181 taaatattca aattttacaa tatataatat tacctaaaga gtatcaatta gttttatgat
      241 ttttattttt tgtagctttt gctgtaaaag taccaatata tccagtgcat agttgattac
      301 ctgaagctca tgtagaagca ccaacaggtg ggtcgataat attagctggt gttttattaa
      361 aattaggctt atatggtatg ttacgtgttt tattagtatt atttcctata ggaaatatat
      421 attatacacc attagtaata actttaagtt ttatttcagt aatttttata tctataataa
      481 tattacaaca aattgattta aaaaaaataa tagcatattc ttcgatagct catatgaatt
      541 ttgttgttat aggtttattt tcaaataatg tatatggtat agaaggtggt atttttacaa
      601 tgttaagtca tggtttaatt tcaagtatgt tatttttttg tataggtgtt ttatatgata
      661 gatatggaga acgtaattta ttatattata ataatatagg acaaataatg ccacatttta
      721 gtgttttttt tttttttggt ttacttgcaa atttaggtat gcctggtaca agtagtttta
      781 ttggtgaatt tttattatta ttaagtttaa gctttaaaag tacttttcta ttatttataa
      841 tgtcattttc tttattttta actacaatat attgtatttg attatttaat agaatatgtt
      901 ttggttatat gcgtaatcgt tatattagag tatataaaga tttaatccga tttgaatcta
      961 ttgttttaat aatattaaca atattaatat ttttttttgg gttatatcca aattgtatat
     1021 taaatttatt tcatgaattt gtatgtttaa atttaaataa acatattctt tttaattcaa
     1081 aatatataag taaataatga actctttttt aataaatatt tacgaatttt atataaaaaa
     1141 tcaatattat ataattacaa tatataacaa atatattagt aacttaggaa atatcttatg
     1201 agataatgtt caatgagata atatatataa tgttttatat atattatttt ttataataat
     1261 aataataagt agtttaggcg ttttattatc aaaaaatcca atacattctg tatttttttt
     1321 aataatagtt tttttgcatg ttagtttttt tttattatta ttgcatgttg aattcttagc
     1381 ttttttaata tttatagtat atttaggtgc tattgctgtt ttatttttat ttgtaataat
     1441 gatgtttaat attcatatat tagaaacacg tgataatatt attagatata tacctttagt
     1501 aataggaatt tttttaataa tattagaaat gagttatata acagattgaa tagatttaaa
     1561 attagaaatt ttatctttta aagatagttt ttatattcat aaaaattgat ataatttatt
     1621 atatagtaaa gataatataa tagttttaag tataatatat aattattatt tttataattt
     1681 tatattagca agtttaatat tattagtagc aatgataggt gcaattgtat taactttaaa
     1741 ttataatgtt gatttaaaaa aacaaatata ttatatgcaa aataataaaa atttatataa
     1801 tagcgtatcg ttaaaaagat caagtaaatt aaagtgagaa ctatgacaaa tttatttgaa
     1861 aaaaaaaaaa taattggagc gtccgttatt tatatatatt aatagttttt tattaatatg
     1921 tattttaggt attttaaata atgtaatatc atatacagaa gaactatcat tatcattaat
     1981 ttatatgagt ttatttatta tttttagtga aggctcaaaa ttaatgataa aaaatataat
     2041 agtatctaat acttttatgt taatgttatg ttatgagcaa ttaatatatt taaaatttaa
     2101 attattaata aaaaacttaa atttatcaag attaatattg gagaaaaaat atttaaaaaa
     2161 tttatttaat aatttagatt ttgaaataaa agataaaaaa aataattttt tagatataaa
     2221 aaaaaatatc aataaattaa aaaatttaaa tcaattatta gtatttatat taaatataaa
     2281 ataaaattaa aaatgataga gtattataaa atatttataa tgtttatagt atgtgtagtt
     2341 ttagcattag taattttagg tgcgtcttat ataataaaat caaaaaatta taattgagaa
     2401 aaaatttcat catatgagtg tggttttcaa ccttttgaag atacaagaag taggtttgat
     2461 gtaaaatttt atttagtagc tatattattt attatttttg atttagaaat tgcttattta
     2521 tttccatgat ctttatgttt agatgaatta aatcttgaag gtgtagtatc tatgataata
     2581 tttttattta ttttaactat aggttttgta tatgaatgaa aaaaaggagc attagattga
     2641 tattagaaaa tgtcatttgc taaatggtta agatcatttt taaaattttc accaagaagt
     2701 tcttatatga aaataagaaa aacatattta ttaaagaatt ataatgatat aaattgatat
     2761 tttatatatt atattatatt aatgttaggt aaatatataa aaaaagtaca attaaaaaaa
     2821 ggtgaatata atttatattt aaaaaataat caagatattg aaaaagtttt atttttttta
     2881 aaatataata taaatagtca atttaaatta ttaatggata tttgtggtat tgataatatg
     2941 caaaaatatg aaaatcgttt tgaaataaat tataatttat tatctgtaaa atattgagca
     3001 cgtatgcatg taaaggttca tgttaatgaa tttacacctg taaattctgt aattaaaata
     3061 tttaaaagtg ctaattgata tgaacgtgaa atatgagata tgtttgggat attttttaaa
     3121 aatcattcag atttacgtag aattttaaca gattatggtt ttgaaggttt tcctttacgt
     3181 aaagattttc ctcaaacagg ttacgtagaa ttaagatata ataatgatta taaacattta
     3241 atttatgaac ctgtagaatt aagccaagat tttagaagtt ttgatttttt aagtccttga
     3301 aatcaaataa aataaaaatg ttaaataaaa taaagcattt ttatatgaat tttggtccgc
     3361 aacatcctgc agcacatgga gtgttacgtt tagtattaga attacaaggt gaaacaataa
     3421 taagagccga cccacatata ggtttattac atagaggaac agaaaaatta attgaatata
     3481 gaaattatat acaaggttta ccttattttg atagattaga ttatgtatca atgatggcac
     3541 aagaacatag ttattcttta gctatagaaa gtttattaaa atgtgttgtt ccaaaaagag
     3601 gtcaattaat aagaatttta ttttcggaaa taacaagaat tttaaatcat ttattagcat
     3661 taaccacaca tgcgttagat gtaggtgcta tgactccttt tttatgaggt tttgaagaac
     3721 gtgaaaaatt aatggaattt tatgaaagag tatcaggtgc tagaatgcat gctgcttatt
     3781 ttagaccagg tggtgtcaat caagatttac caaaaggttt attacatgat atatatttat
     3841 ttttatatca atttgaagct agaataagtg aaatcgaaga attattaaca aataatagaa
     3901 tatgaaaaca acgattagta aacgttggtg ttgtaaatta taaacaagca tttgaatatg
     3961 gttttagtgg tgtaatgctt agatcttgtg gtatagaatg agatttaaga aaacaaatgg
     4021 gttatgatgg ttataatact gtaaatttta gtgttccaac aacttttaat ggtgattgtt
     4081 atgataggta tttaatacgt attatggaaa tgagagaaag tcatgaaata atgaatgaaa
     4141 ttatacaaag attatcaata aaatttaaag aacctctatt aaatttagtt aaaacaaatg
     4201 attataaaat ttgtccacct tcaagaggtt atatgaaaca ttcgatggaa gctttaattc
     4261 atcattttaa attatataca gaaggtattg ttattccaga aaatcaaata tattgtgctg
     4321 tggaagctcc aaaaggtgaa tttggtgttt atttagtttc gaataatact aataaacctt
     4381 atcgttgtca tataagagct cctggatttg ctcatttaca aagtttaaat tttatgtcaa
     4441 ataatcattt aattgctgat ttagtaacta taataggtac tcaagatatt gtttttggtg
     4501 aaatagatag ataaaatgat ttaaatataa atatataatt tataatgtgt tctttaaaaa
     4561 taaaaataat taaatataaa aaataaatac aatgggtgga ataagagatt atacattaca
     4621 aaatattata tttgtgcatg attttgtatt tatattttta atattggtat ttttagtaat
     4681 attagcatat tttgcaactg tgttatatga atatactgat aatagatatg ctgaatattt
     4741 tcgtttattt aaaggtgatt ttgtaatacc aatgaataaa tgactttctt atttaagaga
     4801 agaaatttta atattaagaa aaactaaaga tgtaaaaact atgggtataa atagacgagt
     4861 aataagtaaa tgagcaagta cttcaaatta taatgaattt ttagtaataa aagcatatag
     4921 taataatgta aaaaatatac taaatcaagt tcataattta tcattagagg ttatatgaac
     4981 ttttattcca atagtaataa ttttagaatt aatatttcca tctttatcat taatagctga
     5041 tgatgaaata agtaataaag caagtataca tactgtaaat gttattggaa atcaatgata
     5101 ttgaacttat gaagtaaatg ttgctatggg tattatgaaa actgtaagta atatgttatt
     5161 attatctgat atagataatc atatcttagc aaaagagcat gtacgtttat tatcaacaac
     5221 tgatttagta actatacctg taggtttaaa aacatcattt tttataacat caaatgatgt
     5281 agcacatagc tgagctattc caggattagg tttaaaaata gatgcaatac cgggaagaat
     5341 taatttaaaa actgttatag cttcaagatt aggtttatat gttggtatgt gtagtgaatt
     5401 atgtggaata gagcatggtt ttatgccaat atcattaaaa gtatattcat ttgataaatg
     5461 aggagcaaat atagaatata taactaatca tgataatgtt agttatagca atattgtaaa
     5521 tttatgatat gtataataaa atagtaaata ttttagtata aacatgctaa ttaaaagaaa
     5581 attaatatta taaaaaaagc atttaatgga taccttggga gtaaaaaaaa agacgtaaag
     5641 aaacgaaatg ttatcattgc agaatagtga aagagttacg aagttaaatt cgttttcgat
     5701 gaataacttt cttgaaggaa actataatat aaatttagtg aattgaaaca tcttagtagc
     5761 taaagaaaaa aattaataaa gatttcgtga gtagtggtga atgaaaatga aaaagaaatt
     5821 tattatgtaa attaattgaa aaattaagag taccggtgaa attccattcg taaaacaagg
     5881 taaaaatcct gtaggttaca tttatataat aaaattaata aagtaaaaca aaacctgtga
     5941 aattttgttt gaataaggga gaaccatctt ctaattctaa aatttttact caccgatagt
     6001 gtaaaaaagt accgtgaggg aaaggtgaaa agagcgaaag taagtgaaat agaacttgaa
     6061 attgaatgtt tatagagcag tcagatttag taaataaaga tgatgtacct tttgtataat
     6121 gggtcagtga gttaattttt ttaacaagtt taagttaata aatgtaggca tagcgaaagc
     6181 aagttgtaaa aggcgtatag ttaaaaaaat taaacccgaa actaagtgat ctaattatga
     6241 gcagactgat tgaagctgat aaagcttttt tgagggtcga acttgtgtat gtagcaatat
     6301 actaagatga cttgtgatta ggggtgaaag gctaatcaaa cttagagata gctggttttc
     6361 tgcgaaattt atttaggtaa agtgtaatta aaatattgtt aaaggtaaag ctctgagtag
     6421 gtgcgggggg cttagtcggc cttaccaaat ctaaacaaac tgtgaatatt aacaataata
     6481 ttttttacaa tcagaataaa agtgttaaga ttgttattcg aaagggaaac agcccagatt
     6541 attaattaag gtctttaaat atatttaagt agtaaagaaa taaaatataa attatatcag
     6601 gaggttggct tagaagcagc aggcctttaa agaaagcgta acagctcact gaaaaaaaa
//
LOCUS       DAOQLC010000178         6625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4848_length_6625_cov_26.053272, whole genome shotgun sequence.
ACCESSION   DAOQLC010000178 DAOQLC010000000
VERSION     DAOQLC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4848_length_6625_cov_26.053272"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1181)
                     /locus_tag="PL000_19495"
     CDS             complement(42..1181)
                     /locus_tag="PL000_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91123.1"
                     /translation="MANILLTGGRAPATLDLARVFHRAGHAVFMAESLRGHLSQPSKA
                     IKANFVVPAPRQNRDAFLFALKKIVTENQIDLLVPTCEEIFHTAMGHESLPLFSEPIE
                     KLRRFHNKWEFVVQAVENELHVPETMLIQNKEDLLHAYAQWRGLVLKPAYSRFAARTL
                     ILPPLQMALSTLKFTKGQPWVAQQYVEGQAFCTYSVCHKGRITAHTTYPSHFTAGRGA
                     TIVFKHVEHSAIFNWVRSFVSENQFTGQIAFDFIETPDGQVTALECNPRATSGVHLLA
                     SHPRFAESFFNPFMDCITPVDDSSFMLSAAMLLYGLPAALKNGSLKDWRKAYFFSNDV
                     ILDFNDPLPFILQWRSVLATRAIAKREKLSLLEASTFDIEWNGEG"
     gene            complement(1258..2253)
                     /locus_tag="PL000_19500"
     CDS             complement(1258..2253)
                     /locus_tag="PL000_19500"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNC91124.1"
                     /translation="MQVNIPLKIIGLGRYLPKRVVPSSELEAMCGVPAGWVERRNGVR
                     ERRWVTDETSSFMSAEAAREALSEAKLKPEQLDLIINASGTGEQAIPDTGVLIQRQLG
                     LGASGIPAMTVHTTCLSFVAAMDVASNFISSGRYKNILICSADVASCGINPKEPESAS
                     LVGDAAAAVVVTRAGAGEASQIHHAHFKSFGDGAYLTTIMGGGSRLHPRFAGHNPEDD
                     LFHMDGPAVLRMVRGIAHEFLEELYPGLSNSMVDVDVVVPHQASKVGLMMLERFGWKD
                     EKIIKTIETLGNCVAASIPVTLYQGVRDGRIQRGNKVLLVGTGAGLSIGGMVLTY"
     gene            complement(2340..3503)
                     /locus_tag="PL000_19505"
     CDS             complement(2340..3503)
                     /locus_tag="PL000_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91125.1"
                     /translation="MSNLSGVMTHPPQLFTRENIHTLPFPSTDDGEYARHYLIPLMTG
                     DPQKYIRNVYSTQLMAVKVGETTIPITVSDFHPQNTYTVSPYSHYVSYGAFEEVKHLN
                     NAAAEFAVKAVMNPLAWYFRKAELDRVVFVNNYLLSTNLYPSVNSDQLSAASEALIGW
                     FPDRAIVFRSVDQKKNPHVYRTLEGLGYDLVLSRQVWYMDPVESLKTRQCREDVRVLK
                     KNGYETVNGKDLSDAELHRAVELYNLLYLEKYSYYNPQFTFEFLKLARDHAILHMVGL
                     KKDGQLNGIMGFFVRNGAMTQPLFGYDTSLPMDEGLYRLLTLVTLQEGVKRGLLVHAS
                     GGVGKFKKVRGGESVTEYNAVYTRHLPKRRQLPWKLIGKVSNWAIPYFKKMDF"
     gene            complement(3522..4364)
                     /locus_tag="PL000_19510"
     CDS             complement(3522..4364)
                     /locus_tag="PL000_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC91126.1"
                     /translation="MKINILHAGYCTAPEHIAMQGGRWRTIHFPAMFALFRHPKFGAM
                     LFDTGYSYRFFDETKKFPKRIYRWMTPVHLKEEDLVVNQLSAFNLQSSDISHVFVSHF
                     HADHVGALCDLPKSRYMYFPHAFAHLRHLPPEEDMKYAYLRGQIPTDFDARSQEVDMT
                     RPVLLAEEYAPFKTGYDLLGDGSIIGVELPGHALGQMGIFARDENGKLFFFVADAAWL
                     KQSIVTNRPPHKIANLLFPDPALYRETLGQLHTYYLTHPDTVVVPSHCDETIRLLENA
                     PQQG"
     gene            complement(4403..5398)
                     /locus_tag="PL000_19515"
     CDS             complement(4403..5398)
                     /locus_tag="PL000_19515"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC91127.1"
                     /translation="MKTLVTGATGFVGGALARRLHGMGWNVTALGRNPAKLDQLEEEG
                     IHPLRADISKKDELTDSFLNLDLVFHCAALPSPWGNFEKFYQSNVIGTRNVVQACLEN
                     KVKRLVYVSTPSLYFDYGSRIGVKENDPLPEPISSYAHTKLLAEEEIDKGFAQGLAVV
                     SIRPRALFGEGDTVIFPRLIPRLRSGRLPILGDGENVVDLTYIENVVDALLLCAESPA
                     STLGKKYNITNDEPVKIWKLIERICDELGYRHPSRKISYKTANAAASLLELFYSLIPF
                     SPEPPLTRLSVSMMANSTTLDISAAKNELGYQPKISVEEGVQRFLKWWKEANPSL"
     gene            complement(5474..6562)
                     /locus_tag="PL000_19520"
     CDS             complement(5474..6562)
                     /locus_tag="PL000_19520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HNC91128.1"
                     /translation="MSTSSNSNSFSGAYKHLMTGVSYMIPFVVAGGLLIAIGFAARGI
                     YAYDATLALDGSTMEATAWTTFWANIFAIGAKGGFGLMVAILAGYISYSIADRPGLAP
                     GMIGGTLAGVTGSGFLGGIIAGFLAGYFTQWLNGAIKLPRNLSGLKPVLILPLISSLV
                     VGLVMFYVVGVPVSAALKALTAWLTGMQASSAAILGLILGAMMAFDMGGPVNKAAYTF
                     SVGLLSTQVTAPMATVMAAGMTPPLGLALAAYLFKNRFTEDERESASSTAILGISFIT
                     EGAIPYAARDPFAVIPSIMVGSAVAGALSMAFGCQLHVPHGGIFVLPIPNAVDNLLMY
                     VVALLVGTAVTAGMLYLLKKPVEQAPAA"
BASE COUNT         1697 a         1816 c         1672 g         1440 t
ORIGIN      
        1 aacaccctat atacgtcaaa ttatacgccc ccaaacaaca actatccctc cccattccac
       61 tcgatatcga aagtggaagc ctccaacagc gagagcttct cacgttttgc aattgcgcgc
      121 gtggccagca cactgcgcca ttgcaaaatg aacggcagcg gatcgttgaa atccaaaatc
      181 acatcattgc tgaagaagta agcctttctc caatctttca ggctgccgtt tttcaaggcg
      241 gcgggcaggc catagagcag cattgccgcc gaaagcatga acgaagaatc atccacaggc
      301 gtaatgcaat ccatgaacgg attgaaaaaa gactcagcaa accgcgggtg tgaagccagc
      361 aaatgcactc cactcgtggc acgcggatta cattccaggg cagtaacctg tccatcgggt
      421 gtttcgataa aatcaaaagc gatctgaccc gtaaattgat tctcgctcac aaaagacctg
      481 acccaattaa agatcgccga atgctccaca tgcttgaaaa cgatcgtcgc cccacgccct
      541 gccgtaaaat gcgaaggata ggtggtatgc gcagtgattc gccctttatg gcagacacta
      601 tacgtacaaa acgcctgacc ctccacatat tgctgtgcca cccacggctg cccttttgta
      661 aactttagtg tggataatgc catttgaagc ggaggcagga tcaaggtccg cgcagcgaaa
      721 cgcgaataag caggcttcag caccagtccg cgccattggg catacgcgtg gagcagatcc
      781 tccttgtttt ggatcaacat cgtctcgggc acatgcagct cgttttccac cgcctgcaca
      841 acgaactccc acttgttatg gaagcgcctc aacttctcga tcggctctga aaacaaaggc
      901 aggctctcgt gccccatcgc cgtatggaat atctcctcac aggtcggaac cagcagatcg
      961 atctgattct ccgtcacgat ctttttcaac gcgaaaagaa acgcatccct gttctgacgt
     1021 ggagctggca ccacaaaatt ggccttgatc gcctttgacg gctgactcaa atgtccgcgc
     1081 aaagactctg ccataaagac agcatgaccc gcacgatgaa acacgcgagc cagatcaagt
     1141 gttgcgggcg cacgcccgcc ggttaataaa atgtttgcca ttgttgctta ccgcgaagac
     1201 cgcaaaggac gcaaagattc aaaaaggttt tcttcgcgct cttcgcgtgt ttagcggtta
     1261 ataagttagt accatcccgc caatggacaa ccccgcgccc gtgccgacca acagcacctt
     1321 gttgccgcgc tgaatgcgtc catcgcgcac cccttgatac agtgtaacag ggattgaagc
     1381 cgccacacag ttcccaagcg tttcaattgt cttgataatt ttctcatcct tccagccgaa
     1441 ccgctccagc atcatcaacc ccaccttgct tgcctgatgt ggaacaacca catccacatc
     1501 caccatgctg tttgaaagcc ccgggtacaa ctcttccaga aattcatgcg caatgccgcg
     1561 caccatgcgc agcacagcag ggccatccat gtgaaacaga tcgtcctcgg ggttatgccc
     1621 cgcaaagcgc ggatgcaaac gcgaaccgcc gcccatgatc gtggtgagat acgcgccatc
     1681 gccaaacgat ttgaaatgcg catggtgaat ctgcgaagcc tctcccgccc cggcacgtgt
     1741 caccaccact gctgcagccg catcacccac cagcgaagcc gactcaggct ctttggggtt
     1801 aatgccgcac gaagccacat cggcgctgca aatgaggatg tttttataac gcccgctgct
     1861 gatgaaattc gaagccacat ccatcgctgc cacaaaactc aaacaagtgg tgtggaccgt
     1921 catggcaggg atgcccgaag cacccagccc caattgccgc tgaatcaaca cccccgtatc
     1981 gggaatcgcc tgctcacccg tccccgaagc attgataatg agatcaagct gttcgggctt
     2041 caatttcgcc tcgctcaatg cttcacgcgc cgcctctgcc gacataaacg acgaagtttc
     2101 atccgtcacc caacgacgtt cgcgcacccc attgcgccgc tccacccagc ccgcaggcac
     2161 accgcacatc gcttccaatt ccgaactcgg cacgacccgc ttcggtaaat accttcccaa
     2221 accaatgatt ttgaggggaa tgtttacttg catgtttgct cctaaaagtt acctcgataa
     2281 acagggatga acaaagatgt aaaccaggct ttatctctgt ttatctccgt tcatcaaggt
     2341 taaaaatcca tcttcttaaa atacggtatc gcccaattcg aaaccttccc gatcaacttc
     2401 catggaagct ggcgtctctt tggcaaatgc cgtgtgtaca ccgcattgta ttccgtcacc
     2461 gactcaccgc cgcgcacctt cttaaattta ccaaccccgc cgctcgcatg gacgagtaat
     2521 ccgcgtttca caccttcctg caaagtcacc aatgtcaaca ggcggtacaa accttcatcc
     2581 atcggcagag aggtatcgta gccaaaaagc ggctgggtca ttgcgccgtt gcgcacgaag
     2641 aatcccataa tgccattcaa ctgcccatcc ttcttaagtc caaccatatg caagatcgca
     2701 tggtcgcgcg cgagtttcaa aaactcaaat gtgaactgcg ggttgtagta ggaatatttt
     2761 tcaagataca acaggttata caactccacc gcgcgatgca attccgcatc tgaaaggtct
     2821 ttaccattca ccgtctcata tccgttcttc ttcaacacgc gcacatcctc cctgcattgg
     2881 cgcgtcttca aagactccac agggtccata taccagactt gacggctgag aaccagatcg
     2941 taaccaagcc cttccaaggt tcgataaaca tgcggatttt tcttctgatc caccgaacgg
     3001 aacacaatcg cccgatcagg gaaccagccg atcaacgctt cgctcgccgc cgacaactgg
     3061 tcgctgttta ctgatggata caggttcgtc gaaagcaggt agttattgac aaaaaccacc
     3121 ctgtccaatt cggcttttcg gaaataccac gccagcggat tcatcaccgc cttcacagca
     3181 aactctgccg ccgcattatt caaatgcttc acttcctcga acgcgccata cgaaacataa
     3241 tggctatacg gcgaaaccgt gtaggtgttt tgcggatgaa aatctgaaac cgtgatggga
     3301 atcgttgtct ctccaacttt gaccgccatc aactgtgtgg aatacacatt gcggatgtat
     3361 ttctgcgggt ccccggtcat caaagggata aggtaatggc gggcatactc accatcgtcg
     3421 gtggatggaa acggcagggt atggatattt tcacgggtaa aaagctgtgg agggtgcgtc
     3481 atcacaccag ataagttaga caatgcttct ctcagggctc atcaaccctg ttgcggcgcg
     3541 ttttccagca aacggatcgt ctcgtcacaa tgcgacggga caaccactgt atcggggtgg
     3601 gtcaggtaat aggtgtgcaa ctgtcccagc gtttcgcggt acaaagcagg gtcggggaaa
     3661 aggaggttgg caattttatg cggcgggcgg ttggtcacaa tggattgttt cagccaggcg
     3721 gcatctgcta cgaagaaaaa cagcttcccg ttttcatcgc gggcgaagat gcccatttgc
     3781 cccagagcat gcccgggcaa ttccacacca atgatggagc cgtcgccgag caggtcataa
     3841 cccgttttga agggcgcata ttcctcggcc agtaacacag gcctggtcat atccacttcc
     3901 tgtgagcggg catcgaaatc tgtggggatc tgtccgcgca ggtaggcata tttcatgtct
     3961 tcttcaggtg gcaggtggcg cagatgagca aacgcgtgtg gaaaatacat ataccgcgat
     4021 ttgggcaggt cgcaaagcgc cccgacatgg tccgcatgga agtgggagac aaacacatgt
     4081 gagatatcgg aagattgcag gttgaaagcg gacagttgat ttacaaccag gtcctcttct
     4141 tttaggtgaa cgggcgtcat ccagcgataa atgcgctttg ggaatttttt ggtttcatcg
     4201 aaaaagcgat aggaatagcc tgtatcgaaa agcattgcgc cgaatttcgg gtggcggaat
     4261 aatgcgaaca tggcagggaa atggatcgtg cgccagcgtc cgccttgcat ggcgatgtgt
     4321 tcgggagcag tgcaatagcc tgcatgaaga atattgattt tcatatgaat gtttccgtca
     4381 ttgcttcttc gggctatcac cctcacaatg acggattggc ctccttccac catttcaaaa
     4441 agcgctgcac accttcttca acggagattt tgggttgata accaagttca ttttttgcag
     4501 cagagatatc gagcgtggta ctgttcgcca tcatactgac agagagtctt gtcagcggcg
     4561 gctcggggct gaacgggata agcgaataaa acagttcaag caggctggcg gcggcatttg
     4621 cagttttata ggagatcttg cgcgacggat gccgataccc caattcgtcg caaatgcgtt
     4681 cgatcagctt ccagattttg acaggttcat cattcgtaat attgtatttt ttgccaagcg
     4741 tgctggctgg cgattccgcg cataacaaca gcgcatccac cacattttcg atataggtca
     4801 ggtcaacgac attctccccg tcacccaaaa tgggaagcct gcccgagcgc aagcgtggga
     4861 tcaggcgcgg aaaaataacc gtatcgccct cgccaaacaa cgcacgcggg cgaatggaca
     4921 ccacggcaag tccctgcgcg aaacctttgt caatttcttc ttcggctaaa agctttgtgt
     4981 gggcataact actgatgggt tccgggagcg ggtcattttc ttttacgcca atgcgtgaac
     5041 cataatcaaa gtaaaggctg ggtgtggaga catagaccag acgcttgacc ttgttttcca
     5101 gacaagcctg cacaacgttg cgcgtaccga tgacattgga ttgatagaat ttttcaaaat
     5161 ttccccacgg ggagggcaat gccgcacaat ggaagaccag atcaaggtta agaaaggaat
     5221 cggtcagttc atcctttttg gaaatatcgg cacgaagcgg gtggattcct tcttcttcga
     5281 gctgatccag tttcgctggg ttgcgtccaa gtgcggtcac attccagccc atgccatgca
     5341 agcgacgagc aagagcgccg ccgacaaatc ctgttgcacc tgtgacgagg gttttcataa
     5401 gctgatttta tcgccaattg ggtcaaacaa aaatccccca ccacatgggt gggggatgga
     5461 tcaaacttct ggattaggca gcgggagcct gttcaactgg tttcttcaag aggtagagca
     5521 ttcctgcggt gacagcggta ccgacgagca gggcaaccac gtacatgagc aggttatcta
     5581 ctgcgttcgg gatgggcaat acgaagatgc cgccatgcgg aacatgaagc tggcagccaa
     5641 acgccatgga gagcgcacca gcaaccgcgg agccgaccat gatggagggg atcaccgcga
     5701 acgggtcgcg tgcggcgtag ggaatcgcgc cttcagtgat gaaggaaatg ccgaggatgg
     5761 cggtggagga agcggattca cgttcgtctt cggtgaagcg gttcttgaag aggtacgcgg
     5821 caagagccaa accgagaggc ggggtcatgc cagcggccat gacagtagcc atcggggcgg
     5881 tgacctgtgt gctgagcaaa ccaacagaga aggtgtaagc cgccttgttg acggggccgc
     5941 ccatgtcgaa ggccatcatt gcgccgagga tcaaaccgag aatggcggcg ctgctggctt
     6001 gcataccggt caaccatgca gtgagtgcct tgagtgcagc actgacaggt acaccgacga
     6061 catagaacat aaccaaacca acgaccagcg agctgatgag aggcagaatg agcacaggct
     6121 tcaaaccaga gaggttgcgc ggaagcttga tcgcgccatt caaccactgg gtgaagtagc
     6181 cagcaaggaa gccagcgata atgccgccga ggaagcctga accggtaacg cctgcaaggg
     6241 taccgccaat catgcctggg gccagaccgg ggcggtcagc aatggaatag gaaatgtagc
     6301 ccgcaagaat cgcaaccatg agaccaaacc cgcccttggc gccaatggca aagatgttcg
     6361 cccagaaggt cgtccaggcg gtggcttcca tggtggagcc atcgagggcg agggtggcat
     6421 cgtaggcata gataccacga gcagcgaaac cgatggcgat caaaagaccg cctgcgacga
     6481 cgaacgggat catgtaggac acgcccgtca tgaggtgctt gtaagcacct gagaacgaat
     6541 tgctattgct tgatgttgac attttctctc tccttgaaaa ttatgaattc agagcggcgt
     6601 taatgacgcc aactccatct ttgat
//
LOCUS       DAOQLC010000179         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4849_length_6623_cov_19.903319, whole genome shotgun sequence.
ACCESSION   DAOQLC010000179 DAOQLC010000000
VERSION     DAOQLC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_6623_cov_19.903319"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PL000_19525"
     CDS             complement(<1..253)
                     /locus_tag="PL000_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91129.1"
                     /translation="MTPQPTLRLSLPSKGRLMDDSLDFLTECGLSVQKLNPRQYQAKM
                     PALPELTVLFQRPGDIVVSVRQGSVDFGITGIDVLEENRG"
     gene            687..6608
                     /locus_tag="PL000_19530"
     CDS             687..6608
                     /locus_tag="PL000_19530"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNC91130.1"
                     /translation="MKNQKTLRIIGVIAIFALAFVLTKSIFFNNAPLSIEAEPTGPQV
                     IAQNPIEGQRLTLEPSIQFTFDRDMDKAATEKAFSLLSNGEPVPGQLTWLDTRTFSFS
                     PDSKLTPATEYMAVITTSAIAKDGTSPEEEIKIEFMTVETLSVSQVFPAPDTQEVDLN
                     STITVIFNHPVVPLNIVEEQDKLPQPLKFTPAINGKGEWVNSSVYVFQPEELLTSGTS
                     YQVRVDPKLQDTLENTLGESYIWQFDTRKPIIYNFALKDGEQNPPGDVYNMRLDQSFI
                     VSFEQPMDRKSTEAAFKLINRETKAVFPVSFSWNDESTELVIEPKSNFKIASFYELTL
                     NDTAQAVDSGTLREGLNVGLATVPLPSITSVYPPKDSDNADYRSTIEIQFASQMDLES
                     LKGRVQITPALQGGEDDWYYNSYNHTLNIYGLEPATRYAVRILPGMADIYGNTIQDEL
                     SYAFYNGDYATVANIAMPWNNPLIYRAHGTQEVFLEYQNINEATLAIYPISFTEFSRF
                     NSGNAVPAQYDPNEKPIREWKIAAGERNKLERELFNFQDKNGASLDAGYYFIGLKVKP
                     FKYNDNFFQAFTFIVANDNITLKATPTEGFAWVTDLESGAPRSNVAVTFYDDKFQPLG
                     KVNTDKNGIAHLKNIKSVNYAQIQDSSDDFGFTALYWGSGVSAGTFNLYGNYYSSVDK
                     LFGYLYTDRPIYRPDQEVYFKGILRQDDDLHYSHPRINQVYVVVEQWGEKIYEDYVPI
                     NDQGSFSGKVKLDADVSLGYYMVYAYVSNSPNEGPITSVGFSVAEYKKPEFEVLVSPD
                     STEVLMGEEVNFGLDAEYYSGGLLKNASVEWFIEARPYYFHPIEKYNRYSFMDWNRDM
                     YWEPETQSGNGTLAEGSGETDENGHFDIAQILESSKENEGISQTMTLSANVADVAGNT
                     VSGTGSVVVHHSEYYAGIRSEKYVATQGEAQAFNLVVLDWNSQPIADQKVTVSFVERR
                     WYSIQKQDEQGMLQWETSVKEIPVSSVEATTGADGTAQVEFTPPNGGVFKALVKVIDT
                     KGNTHQASTYLWVSSKEQIFWRQTNDRAFNLIADKDSYSPGDTAEILIAQPFQGDVYA
                     LITYERGHIYKSEVVKLNGNSTIYHLPITEEMVPAAYVSVTVISGAQNSGAPDFKMSI
                     ARLNVDTELKTLDVSVSANKESAGPGDKVTYTIETKDSKGAPVSADVSLSVVDKAVLA
                     LMPSNTIPLLSSFYATRGLGVMTALGIVSSADDFNANYRKTIPDGNKSGGGGGGEPGV
                     ITVRENFKDTAAFEAEVMTDENGIAKVDVELPENLTTWVAEARAITEDSRVGQATSEL
                     VSSKPLFVQLQTPRFFVVGDQLTVGATVFNNLETSLKVDISLDAEGVQVLSDPKQSIE
                     IEGRQQAYVTWDIVVKANVERVDLTATAISGQFTDASKPALGTLDGQGIPVYNFTAVE
                     TVGTSGMILTADSVTESIQLPTTLNIEDANLSIEISPSLAASMQSSLKYLEDYPYLCM
                     EQTISRFLPNVITMRALDAAGRPTPMRGSLNTQVNSALQRIYAKQKYDGGWNWWDGEL
                     SDPQTSAYVIYGLIEAQASGYEISEIVLANGLDYLKNNLPDIKRNEASWHYNRYAFML
                     YVLARADELDAGKTNFIFENHSKLDLYGKAYLAQAMFMLDEEDPRIKTLLSDLVTATV
                     QSASGAHWEESTKDYWNWNTDTRTTAIVLNAFVQIDPQNPITANAVRWLMAHRESGHW
                     QSTQETAWSLIALTNWLIESQEYETDYKYAVGLNGNLLDEGQASAENLTETVNLQVQI
                     EDLLKEQGNDLVITRGRGTGNLYYSAYLSTSLPVDSIQPLDQGVSLSREYFTLDDPKT
                     PITEIERGELVKARLTIVIPAAVHYIVIDDPLPAGMEVLNASLATDTAVPSFYSAQDY
                     KDRGWGWWYFSHIELRDEKITLSTDYLPAGTYVYTYIVRASTAGTFNVIPPTASEFYF
                     PDVGGRGAGSVFTVR"
BASE COUNT         1805 a         1777 c         1607 g         1434 t
ORIGIN      
        1 cgccgcgatt ttcttcgagc acgtcgatgc ccgtgatgcc aaagtccacg ctgccttggc
       61 ggacgctgac gacgatgtca cctgggcgtt gaaagaggac agtcaactcg ggcagggcgg
      121 gcatcttcgc ctgatactgg cgcggattca gtttctgcac agagagtccg cactcggtga
      181 ggaagtcgag cgagtcatcc ataaggcgtc ctttggaggg gagggagaga cggagggtgg
      241 gttgtggggt catgtaaggt ttcctttaat tttgtgtctg gtccccctcc gcctgctatc
      301 gcaggcacct cccccaaata cgacggacga acttccgtcg tatttggggg aggacgggtg
      361 aggggtaaca aaaaaccccg acacagggtc ggggcttgat gaggtgaagt atgggtatct
      421 gctatttagt cacaggtacg cgcacataaa cctcccgacc atgcgggggt tgaatatgat
      481 gatggtggtg cttgagcgcg gacttgttca tgttgggcgg cagtatacca acgggagggc
      541 aattctgcaa gccattgcgt tttttcgaat tgcggtatcg ggaacctctc acacctgaaa
      601 ttcgtttaga gaaaagccct aaaacctaaa aagggtcact tgacatctct cttcacaaaa
      661 ggcatactcg tttgggaggc ggcaccatga aaaatcaaaa aactttgcgc atcattggcg
      721 taatcgccat ttttgcactg gcatttgttc taacaaaaag catctttttc aacaatgccc
      781 ccctgtccat cgaagccgag ccaacggggc cacaagttat cgcgcaaaat ccgatcgagg
      841 gacaacgcct gactcttgaa ccctccatcc aattcacatt cgatcgcgat atggacaaag
      901 ccgcgacaga aaaagcattc tcgcttctct caaacgggga gcctgttccc ggtcaattga
      961 catggctcga cacccgcaca ttttccttct cccctgattc caagttgaca cccgccactg
     1021 aatacatggc ggtcatcacc acctcagcca ttgcaaaaga cggtacatcc ccagaagaag
     1081 agatcaaaat cgaattcatg accgtggaga cactttccgt gtcacaggtc tttcccgcac
     1141 ccgacactca agaagtagac ttgaactcga ccatcacggt cattttcaat caccccgttg
     1201 tgccgctcaa catcgtcgaa gaacaggaca aactccccca accgttgaaa ttcacacccg
     1261 ccatcaacgg caaaggtgaa tgggtgaatt catcggtgta tgtgtttcag ccagaggaat
     1321 tactgacaag tggaaccagc tatcaggtaa gggttgatcc gaagctacag gacacactcg
     1381 aaaacacgtt gggagaatcc tacatctggc agttcgacac acgcaagccg atcatctata
     1441 attttgcttt gaaagatgga gaacaaaacc cgcctggtga tgtgtacaac atgaggctcg
     1501 accagagttt tatcgtctct ttcgaacaac ccatggacag gaagagcacg gaagcggcgt
     1561 tcaaactcat caaccgcgag acaaaagctg tcttccccgt gtctttttcg tggaacgacg
     1621 aaagcacgga attggttatt gaaccgaaat ccaacttcaa gattgcaagt ttctacgaat
     1681 tgaccctcaa cgacactgca caagccgtgg atagtggaac gctgagagaa ggtctgaatg
     1741 tgggactggc aactgttcca ttgccttcga tcacaagtgt ttatccgccc aaagactcag
     1801 acaatgcaga ctaccgctcc accattgaga ttcaattcgc ttctcaaatg gatcttgaaa
     1861 gcctgaaagg tcgggtgcaa atcactcccg ccctgcaagg cggcgaggat gattggtatt
     1921 acaacagcta caatcacacc ttgaatattt acggtcttga gcctgccacc cgttatgcag
     1981 ttcgaatact tccaggcatg gctgacattt acggaaacac aattcaagac gaactttcct
     2041 atgccttcta caatggagat tatgccacgg tggcaaacat tgccatgcca tggaacaacc
     2101 ccttgatata ccgagcacat ggaactcagg aagtgtttct cgagtatcaa aacatcaatg
     2161 aggcaaccct tgccatttac ccgatcagtt ttacagagtt cagccgtttt aacagcggca
     2221 acgcagtccc tgcacaatat gacccaaacg aaaaacccat acgggaatgg aagatagccg
     2281 caggagaacg aaacaaactt gagcgcgagt tattcaactt ccaggataaa aatggcgctt
     2341 cgttggatgc tgggtattac ttcattggct taaaggtaaa accttttaaa tacaacgaca
     2401 atttctttca ggcatttaca ttcatcgtgg caaatgacaa tatcacgctc aaggccacac
     2461 ccaccgaagg gtttgcctgg gtgacagacc tggaaagcgg cgcaccacgc agtaatgtgg
     2521 cagtcacttt ttacgacgac aaattccaac cgctgggcaa agtcaacacc gacaagaacg
     2581 gcattgccca tctgaaaaac attaagtcag tcaattacgc ccagatacag gatagcagtg
     2641 atgattttgg gtttacggcc ttgtactggg gcagcggcgt cagcgcgggc acgttcaatt
     2701 tgtacggaaa ttattacagc agtgtggata aacttttcgg gtatctatac accgaccgcc
     2761 caatatatcg tcccgatcag gaggtttact tcaagggcat cctacgtcag gatgacgacc
     2821 tgcattacag ccatccacgc attaatcagg tatatgttgt cgttgaacaa tggggcgaaa
     2881 aaatatacga agactatgta cccatcaacg atcagggaag tttcagcggc aaggtaaaac
     2941 ttgatgctga tgtttctttg gggtattaca tggtctacgc atacgtatcc aattctccaa
     3001 acgagggacc catcacatcg gttggtttca gcgttgccga atacaagaag cctgaattcg
     3061 aggtgttggt ctcacccgat tcgaccgaag tcctcatggg cgaggaggtc aattttggac
     3121 tggatgcaga atactattct ggtggtctct tgaaaaacgc ctcggtggaa tggttcatcg
     3181 aagcaagacc gtattatttc caccccattg aaaagtacaa ccgttacagt ttcatggatt
     3241 ggaatcgcga tatgtactgg gagcctgaaa cccaatcagg gaatgggaca ttggcggaag
     3301 gcagcggcga aaccgacgag aacggccatt ttgacattgc gcaaatactc gaatcttcaa
     3361 aagaaaatga aggcatttcg caaacaatga ccctctcagc caatgtcgct gatgtcgcag
     3421 gaaacaccgt cagcgggaca gggagcgttg tggtccatca cagtgaatat tacgctggta
     3481 ttcgctcgga aaagtatgtt gctacgcaag gggaggcaca ggccttcaac ctcgttgtcc
     3541 tcgattggaa ctcccagccc attgctgacc aaaaagtgac cgtcagtttt gtggaaaggc
     3601 gctggtacag catccaaaaa caggatgaac aagggatgct ccaatgggaa acttccgtaa
     3661 aagagattcc cgtcagttca gtggaagcca caacaggcgc agatggaacc gcacaagttg
     3721 aattcactcc tcccaatgga ggcgtgttca aagccctcgt caaagtgatt gacacgaagg
     3781 gaaatactca tcaggcatcc acttatctct gggtatccag caaagagcag atattctggc
     3841 gacagacaaa tgaccgtgca ttcaacctca tcgccgacaa agacagctat tcaccagggg
     3901 acacagccga aattctcatc gcccaaccgt ttcaggggga tgtgtatgcc ctcatcacct
     3961 acgaacgtgg gcacatctac aagagcgaag ttgtcaaact aaacgggaac agcaccatct
     4021 accacctccc cattaccgag gaaatggtcc ccgcggctta tgtttcggta accgtcatca
     4081 gcggagccca aaacagcggt gcacctgatt tcaaaatgag catcgcccgt ctcaatgtgg
     4141 atactgaact taaaaccctt gatgtttcag tctctgccaa taaagaatca gcaggccccg
     4201 gagacaaagt tacatacacg atcgaaacaa aagacagcaa gggcgcaccc gtctctgcag
     4261 atgtatcgct ttcggtagtg gacaaggccg tgctggcact catgccttcc aacaccatcc
     4321 cattgttaag tagcttttac gccacccgcg gactgggcgt catgacggca ttgggcatcg
     4381 tttccagtgc cgatgacttc aatgcaaatt atcgcaagac cattcctgac ggcaacaaaa
     4441 gcggcggcgg tggcggcggc gaacctggcg tcatcactgt gcgtgaaaat ttcaaagaca
     4501 cagccgcatt tgaagcagaa gtgatgaccg atgaaaacgg tatcgcaaag gttgatgtgg
     4561 aattgcctga aaacctcacc acctgggtgg ctgaagcgcg agccatcacc gaagacagcc
     4621 gtgtgggtca ggcgaccagt gaattggtca gctcgaaacc gctctttgtg caactgcaaa
     4681 cgccgcgatt cttcgtcgtt ggggatcagc taacagtggg tgcgaccgtc ttcaacaatt
     4741 tggaaacatc cctcaaggtg gatatctcac tggatgcgga aggtgtgcag gttttgtctg
     4801 atccaaaaca aagcatcgag atcgaaggca ggcagcaggc ttacgtcacc tgggatatcg
     4861 ttgtcaaagc aaatgtcgaa cgcgtggatc tgaccgccac cgcgatcagc ggacaattca
     4921 ccgatgccag caaacccgca ctgggcacac tcgatgggca gggcattccc gtgtacaact
     4981 tcaccgccgt cgaaacggtc ggcacttcag gcatgatcct caccgcagac tcggtaactg
     5041 aaagcatcca actgcccacc actttgaata tcgaagacgc gaatctttcc atcgaaattt
     5101 caccatcgtt ggccgcttcg atgcaatcga gtttgaaata ccttgaagat tatccctacc
     5161 tgtgcatgga gcagaccatt tcacgcttcc tgcccaacgt tatcaccatg cgcgcactgg
     5221 atgccgcagg acgccccact cccatgcgcg gctcgttgaa cacgcaggtc aattcggcac
     5281 tgcaaagaat ttacgcaaaa caaaaatacg acggcggctg gaactggtgg gacggcgaat
     5341 taagcgaccc gcagacctct gcatatgtca tttatggttt gatcgaagcg caggccagcg
     5401 gatacgaaat ctctgaaatc gtactcgcca acggcctgga ctacctgaag aacaatctgc
     5461 ctgacatcaa acgcaacgaa gcctcgtggc actacaaccg ctacgccttc atgctctacg
     5521 tgctcgcccg tgccgatgaa ctcgatgcag gcaagaccaa cttcattttt gaaaatcact
     5581 caaaattgga tctctacggg aaagcctatc tggcgcaggc gatgttcatg ctcgatgagg
     5641 aagacccgcg catcaaaacg cttctttccg acctcgtaac tgccaccgtc caatcggcat
     5701 caggcgccca ttgggaggaa tccaccaagg actactggaa ctggaatacc gacacacgca
     5761 caaccgccat tgtgctcaac gccttcgtgc agattgatcc acaaaacccc atcactgcaa
     5821 atgcggtccg ctggttgatg gcccatcgtg aaagcggaca ctggcaatcc acgcaggaaa
     5881 cggcatggtc actgatcgca ttgaccaatt ggctgatcga gtcgcaggaa tatgagaccg
     5941 attacaaata cgccgtcggc ttgaatggca acctgcttga tgaaggtcag gcaagcgccg
     6001 aaaatctgac agagaccgtc aacctgcaag tacagatcga agacctgctc aaggaacagg
     6061 gcaatgacct cgtcatcacc cgtggacgcg gcacaggcaa cctgtattac agcgcctatc
     6121 tcagcaccag cctgcctgtg gattccatcc aaccgctcga tcagggtgtg agcctttccc
     6181 gtgaatactt taccctcgac gatcccaaga ctcccatcac cgagatcgag cgcggcgaat
     6241 tggtcaaagc gcggctgacc atcgtcatcc ctgccgccgt acattacatc gtcattgacg
     6301 atcccctgcc cgcaggcatg gaagtcctca atgccagtct cgcaactgat accgcggtgc
     6361 catccttcta ctccgcacag gattacaaag accgcggctg gggctggtgg tacttctccc
     6421 atatcgaatt gcgcgatgaa aaaataaccc tctccaccga ttatctgcca gcaggaacct
     6481 acgtgtacac ctacatcgtc cgcgccagca cggcaggtac gttcaacgtc attccaccca
     6541 ctgcatccga attctacttc cccgatgtcg gcggaagagg cgcagggagt gtgtttacgg
     6601 tgagataaaa acccagcccg aca
//
LOCUS       DAOQLC010000180         6539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_4958_length_6539_cov_21.563233, whole genome shotgun sequence.
ACCESSION   DAOQLC010000180 DAOQLC010000000
VERSION     DAOQLC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_6539_cov_21.563233"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PL000_19535"
     CDS             complement(<1..1040)
                     /locus_tag="PL000_19535"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC91131.1"
                     /translation="MQAQNDSSRILRVAAWIWIGYLVAMLMMDFVLYTPFAQQAITQN
                     PPLQQDIPGQQLPPFGNQQPLPPSGRFVPIFLFYAANGAVAFLFLLFTYWKWPQDQFG
                     GVFYPLLVLMISTTPIIINTVIVPRFPQGPLSNAEGMALRQLPVLFVALALVAWEYQL
                     PHVIFFSVATTALELGLIVFNVIDTQSVYVFIFIAIIRTITFVATGIFLNLLVTRLRE
                     QQESLQQANANLTHYASTLEQLTVSRERNRLARELHDTLAHSLTAISVSLETAKAYFD
                     IEPKKAQEFLDKSLDATRTGIDETRRALKALRSSMLEDMGLGPAIRQVAESAATRFKL
                     DLDLALPNPMPSL"
     gene            1039..1161
                     /locus_tag="PL000_19540"
     CDS             1039..1161
                     /locus_tag="PL000_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91132.1"
                     /translation="MDKSYHEGGWMPCARRHDSPTFPMTAVMGGCEEFTPANIK"
     gene            1269..1700
                     /locus_tag="PL000_19545"
     CDS             1269..1700
                     /locus_tag="PL000_19545"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HNC91133.1"
                     /translation="MAALSGTSTNKEIERFSRFLTVGAVGTLLDFSLLTLLKLAGLPT
                     LLANSLSFTAGLVNNFTWNRLWTFGDTVQPDWRRQLAQFTVVSLVGLALNNLIVLSLE
                     DSLGAMLGHSNWGYLPAKVIATGVVVFWNYFANRAWTFRLK"
     gene            1791..>2545
                     /locus_tag="PL000_19550"
     CDS             1791..>2545
                     /locus_tag="PL000_19550"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HNC91134.1"
                     /translation="MKMRSLILVMSTMALLLASCGGQPSPAAPQQGDIPQQQPPTPSN
                     EQNQPPQNQNPQTSATLSESQEAAQVAQWVKDLPVVGVDENHFSVIGTISMHMLHNNV
                     RDTIMQQGTAAFYADPALVAQAETDFQNRMNSVLPAMQDGGLLYLRDVQNFPWGLLEP
                     QQGEYHFELSDAIVNEVHAQGLEYVGVVMPFAGWDLAANGAADEICTHFFQEDYPYLA
                     YEGAMGRYADLDAFVTFLGKLVERYDGDGVDDAP"
     assembly_gap    2546..2645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2646..3192
                     /locus_tag="PL000_19555"
     CDS             <2646..3192
                     /locus_tag="PL000_19555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HNC91135.1"
                     /translation="QDVIAIHYNEGKHDANGGTVEDFETQIQAMYALGADKPMWVTEF
                     GVLVGENDTQGRFTRLPEKDAAAWYLRFYTAGLANGVDRFFSDNISFHDTQKNQILLP
                     FYVNKLLEAKLNGFTEAEKIADGQYRFTVNGSPVYILWNGIPSELSGSVTATDMYGNT
                     QTVDAATLQPSEGSPLIVEIP"
     gene            3300..5681
                     /locus_tag="PL000_19560"
     CDS             3300..5681
                     /locus_tag="PL000_19560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC91136.1"
                     /translation="MTTATIPTQPAFLKKNLFLGITPAFLLVIVLMAVSFGVHMVNID
                     SIGDANSYYTAAVEAMLKSWSNFFFVAAEPGGSVTVDKPPLGLWIEAVFAYFLGVSGF
                     SVSLPNILAGVLSIPLLYVMVKKYAGELAGLLAAFVMAFTPVFVATNRNNTMDGMLVF
                     FLLMAAWAFIKATEDGKLRWLLLGGFIVGLGFNIKMMQAFLPLPAFYTLYFFGSKEGW
                     IRKTINLGIATALLLAVSLSWAIVVDLTPADSRPYIGSSENNTVMGLIFGHNGASRLG
                     NTGLGGNGGPQNGTPPTAPQPFDQTQGGPGQTTDQATQPQQNATNAGGPPQEALTACE
                     GSTQGAACSFEMPFGTVNGSCIIPLNSNELACAPQQGQAAPNNQQNGQDNRQGPPQAA
                     LDACSTSTQGDACSFTLQNGNTINGSCITPPNSSELACAPQGMNPQQNGQGPDGGPGG
                     NGGTPFSQETGTPGVFRFFTSPLSKQMSWLLPFALISVVLALFAGKIRLPLESAVHKA
                     LVIWGGWLLTCVVFFSMVSGIFHSYYAIMLAPALGAMVGIGFAQLWSWGGDKKWIGAV
                     LIGASAVTLAFQLFASYQYGEQSWWMLLAVILFAVGTLSMFFVKRAAYLSLLASMLII
                     PMYWTLMTVSTHGNQSLPTAYTGSNSQQQNGPNAPRPQGDGGPNSNDSSEMLTYLQAN
                     TQDVEYLVAVPSSQQGSSWVIQTGRPVLYMGGFGGQDDVVSAEDLAEMVANGELRYVL
                     YGGDRGNKEDIANWLASSCSVVSEFSNQSNGQNQAQGPDGGGPNQASTLYMCK"
     gene            5887..>6539
                     /locus_tag="PL000_19565"
     CDS             5887..>6539
                     /locus_tag="PL000_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91137.1"
                     /translation="MKNKTLFLISILALLTLVISACASIAPATQAAEVQPAQPTVTVE
                     AVNLSQAQPSAAPLEPNMQSLQPAPDAGTQPLNAQPQGNQPMQNGQGGQPNQNNDCRP
                     DLTTAATTLGITAEELQAALGNPAQGKPDLAAAAATLGVTEDALKQAIQQAMPANCNT
                     MGGGAGNGQQPPQEAYDACIGLSEGAACTVLTPNGSLSGTCTLPPVNASTQLACAPI"
BASE COUNT         1582 a         1843 c         1627 g         1387 t
ORIGIN      
        1 agagaaggca tggggttcgg cagagccagg tcaaggtcca gtttgaagcg agtggccgca
       61 gattcggcca cctgtcggat ggcgggtccc aatcccatat cttccagcat ggatgaacgc
      121 aaggctttca aagcgcggcg ggtttcatca atgccggtgc gggtggcatc caatgactta
      181 tcgagaaatt cctgcgcttt tttaggttcg atatcgaaat aggctttggc ggtttcaagc
      241 gaaacggaga tcgcagtgag ggaatgagcc agcgtgtcgt gcaattcacg ggcgagacga
      301 ttgcgttccc gactcaccgt caactgctcc agcgtcgacg cataatgcgt gagattggca
      361 ttggcctgtt gcaaagattc ctgctgctcg cgcagacggg tgaccagcag gttaaggaag
      421 atgcccgtcg ctacaaatgt gatggtgcga atgatggcga tgaaaataaa cacataaacg
      481 ctttgcgtat caatgacatt gaaaacgatc aacccaagtt caagcgccgt ggtggccacg
      541 ctgaaaaaaa tgacatgcgg aagttgatac tcccatgcca ccaatgccag agccacgaac
      601 agaacaggca gttgacgcag cgccataccc tccgcatttg aaagcggacc ttgcgggaag
      661 cgcggcacga ttacagtatt gatgatgatg ggtgtggttg aaatcatcaa caccaacagc
      721 ggataaaaca ccccgccaaa ctggtcttgc ggccatttcc aatacgtgaa taacaggaag
      781 agaaaagcaa ccgcgccgtt ggcggcataa aaaaggaata tcggcacaaa acgccccgac
      841 ggcggcaggg gttgctggtt tccaaagggc ggcagctgtt gaccggggat atcctgttgg
      901 agtggcgggt tctgtgtaat ggcctgctgg gcaaatggag tgtacaaaac aaaatccatc
      961 atcaacatgg caaccaggta gccgatccag atccatgcgg cgacacgcag aattcgagag
     1021 gagtcgtttt gggcttgcat ggacaaatct tatcatgaag gcgggtggat gccctgtgcg
     1081 cgtcgtcatg attcccccac atttcccatg acagctgtca tgggtggttg tgaggaattc
     1141 acgcctgcaa acatcaagta atgactggct tcactctctc attttggccg aaaaatataa
     1201 gattgggata tcgaaagcag caacgtcttt gttcacctct gcttatttct ggttcaaagg
     1261 agcaaaaaat ggccgcttta tctggcacat ccacaaacaa ggaaatcgaa cgcttctcac
     1321 ggtttctgac cgtgggcgcg gtcggcactt tattggattt cagcctgctg accctgctga
     1381 aactggcagg cctgcctact ttgctggcaa acagtttgtc tttcacagca ggcttggtca
     1441 acaacttcac ctggaaccgc ctgtggacct tcggcgacac agttcaaccc gattggcgcc
     1501 gtcaactggc tcaattcacg gttgtaagtt tggtggggct tgcattaaac aacctgatcg
     1561 tactttcgct tgaagacagc ctcggcgcaa tgctcggcca ttccaactgg ggttacctcc
     1621 ccgccaaggt cattgccaca ggtgtggttg tgttttggaa ttattttgcc aaccgcgcat
     1681 ggaccttccg cttgaaatag gtttatggaa acacccaccg cacacgagac gatttgagtc
     1741 tcttttttac cgacaaaacc cgcggtgtat ttgaacaagg agaagaaaac atgaaaatga
     1801 ggtcattaat tttggtaatg tctacaatgg cactgttgct ggcttcctgc ggcggccaac
     1861 catcccctgc cgccccgcaa cagggagata ttccacagca acagccaccc accccatcga
     1921 acgaacagaa ccagccgcct caaaaccaaa atcctcaaac ttcagccacg ctgtcagaat
     1981 ctcaagaagc ggcccaggtg gcccaatggg taaaagacct gcccgtggtt ggtgtggatg
     2041 aaaatcattt ttcagtgatc ggaaccattt ccatgcacat gcttcataac aacgtgcggg
     2101 acacgatcat gcaacagggc acggctgcct tttatgccga tcctgcactt gtggcccaag
     2161 ctgaaacgga ttttcagaat cgcatgaaca gcgtcctgcc tgccatgcag gatggcggtt
     2221 tgttgtacct gcgtgatgtc caaaattttc cctggggatt acttgaaccg caacagggcg
     2281 aatatcattt tgaattaagc gatgccattg tcaatgaagt tcatgcgcaa ggactggaat
     2341 acgtgggagt ggtcatgccc ttcgcaggct gggaccttgc cgcgaacggg gcggcagatg
     2401 aaatctgcac acatttcttt caggaagatt atccctacct tgcttatgaa ggcgccatgg
     2461 gccgctatgc cgacctggat gcctttgtta ccttccttgg aaaattggtt gaacgctacg
     2521 acggcgatgg cgtggatgat gcgccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnntcagg atgtcatcgc catccactac aacgaaggca aacatgatgc caatggtgga
     2701 accgtggaag atttcgaaac gcagatccag gccatgtatg cccttggtgc agataaaccc
     2761 atgtgggtca cagaattcgg cgttctagtc ggtgaaaatg atacacaggg acgtttcacc
     2821 cgcctccccg aaaaagatgc cgccgcctgg tacctgcgtt tttatactgc cgggctggcc
     2881 aacggcgtgg accgattctt ctcggacaat atttccttcc acgatacaca aaagaaccag
     2941 atcctgctgc cgttctatgt aaacaaattg ctggaagcca aactcaacgg cttcaccgaa
     3001 gctgaaaaga tcgccgacgg acaataccgc ttcaccgtga acggcagtcc tgtatatatc
     3061 ctctggaacg gaatcccatc cgaactaagc ggctccgtaa ctgcaacaga tatgtacggc
     3121 aacacgcaaa ctgtcgacgc tgccactttg caacccagcg aaggatcgcc gctgatcgtg
     3181 gaaattccat aaaacaaacg tagatcaaag gttttgtcgg taaaaaaaga ttcaaatgct
     3241 caaacacccc gcgcggcgaa aaggacttaa acaataccgc gcaaaagaag gaacaatcaa
     3301 tgacaactgc aaccatcccc acccaacctg ccttcctgaa gaagaacctc ttcctcggga
     3361 tcacccccgc ctttctgctt gtgatcgtcc tcatggcggt ttctttcggc gtacacatgg
     3421 tcaacatcga ctccatcggc gatgccaact cgtattacac cgccgctgtg gaagccatgc
     3481 tgaaaagctg gagcaatttt ttcttcgtcg ctgccgaacc cggtggctcg gtcaccgtgg
     3541 ataaaccccc gctgggactg tggatcgaag cggtcttcgc atacttcctc ggcgtaagcg
     3601 gattcagcgt ttcgctgccg aacatcctcg cgggcgtgct cagcattcca cttttgtatg
     3661 tcatggtcaa aaaatacgca ggcgaacttg caggcttgct tgccgctttt gtcatggcgt
     3721 tcacacccgt cttcgtggcc accaaccgca acaacaccat ggatgggatg ctcgtcttct
     3781 ttttgctcat ggcagcctgg gctttcatca aagcaaccga agatggcaaa ctccgctggc
     3841 tcttactcgg cggattcatc gtcggtctcg gcttcaacat caaaatgatg caagccttcc
     3901 tgccgcttcc cgctttctac acactgtatt tcttcggttc caaagaaggc tggatccgca
     3961 aaaccatcaa cctcggcatt gccaccgcat tattgctcgc ggtgtcactc tcatgggcga
     4021 tcgttgtaga tttgaccccc gcagactctc gtccctacat cggctccagc gaaaacaaca
     4081 cagtcatggg attgatcttc ggccacaacg gagcctcacg cctcggaaac accggtctgg
     4141 gaggaaatgg cggcccgcaa aatggaacgc ctcccaccgc gccgcaaccc ttcgatcaaa
     4201 ctcagggcgg gcctggtcaa acaaccgatc aggcgactca gccccagcag aatgcgacca
     4261 atgcaggcgg cccaccacag gaagcgctca cagcctgtga aggatcaaca cagggagccg
     4321 cctgttcttt cgagatgccg ttcggaaccg tgaatggctc ctgcatcatc ccgctcaatt
     4381 ccaatgaact cgcctgcgcc ccgcaacaag gacaggccgc ccccaacaat caacaaaacg
     4441 gacaggacaa tcgtcagggc ccgccacaag ccgcattgga tgcctgttcc acatctactc
     4501 agggcgatgc ctgctcattc actctgcaaa acggaaatac gatcaacggc tcttgcatta
     4561 cgccccccaa ttccagcgaa ctcgcctgcg cgccgcaagg tatgaatcct caacaaaacg
     4621 ggcagggacc tgatggcggc ccgggcggca atggcggcac tccctttagt caggaaacgg
     4681 gcacaccggg cgtcttccgc ttctttacca gcccgctctc caaacaaatg tcgtggctgc
     4741 tgcctttcgc gttgatcagt gtggtgctgg cgcttttcgc gggcaagatc cgcctgccgc
     4801 ttgaatcggc ggttcacaaa gcccttgtca tctggggcgg atggttgttg acctgtgtgg
     4861 tgttcttcag catggtctct ggcatcttcc actcttacta tgccatcatg ctggctcccg
     4921 ctctcggcgc catggtaggg attggctttg cccaactgtg gagttggggc ggcgataaaa
     4981 aatggatcgg tgcggtactc atcggtgcat cggcggtgac tctggccttc caactctttg
     5041 cttcatacca gtatggcgaa caatcctggt ggatgctcct tgcggtgatc ctgttcgccg
     5101 tgggaacgct ttcgatgttt tttgtgaagc gcgcggccta tctcagcctg ctggcttcga
     5161 tgttgatcat ccccatgtac tggacgctca tgaccgtatc cacgcatggc aaccaaagcc
     5221 tgcccacagc ctacacaggc agcaacagcc aacagcagaa cggacccaac gcaccccgcc
     5281 cccaaggaga tggtggaccg aactcgaacg acagcagcga aatgctcacc tacctgcaag
     5341 ccaacacaca ggatgtggaa tatctggtgg cagtgccttc ttctcaacaa ggttcctcct
     5401 gggtgattca aacagggaga cctgtgttgt acatgggcgg cttcggcgga caggatgatg
     5461 tggtcagtgc ggaagacctg gcagaaatgg tagccaacgg cgaactgcgc tacgtccttt
     5521 acggcggcga ccgcggcaac aaggaagaca tcgcaaactg gctggccagt tcatgttcgg
     5581 tggtctctga attcagcaac caatccaatg gtcagaatca ggcacaaggt ccagatggag
     5641 gcgggccgaa tcaggcttcg accttgtaca tgtgcaagta atcccaattt ttagacacaa
     5701 aaaacgctga cgaagcaacg tcagcgtttt ttgttttcac aggtactcca ttcttcccat
     5761 gacacctgtc atgcccgttg atgcaagcct gcatggacgt tcgctcaaca tgatgatttt
     5821 acacactcat tacatttttt acgcgtggct aaaatgcagc cacatcaaat ttgaaaggaa
     5881 aacaaaatga aaaacaaaac tctcttcctg atctccatcc tcgccctgct gactctcgtt
     5941 atcagtgcgt gtgcaagcat tgcccccgcc actcaggccg ctgaagttca gcctgcccag
     6001 ccaactgtga ccgtggaagc ggtgaacctt tcgcaggccc agccatccgc tgccccgctt
     6061 gagccaaata tgcaatctct tcaacctgct ccggatgccg gcacacagcc gctgaatgcc
     6121 cagccacagg gaaaccaacc catgcaaaac gggcagggcg gacaacccaa tcaaaacaac
     6181 gattgccgcc ccgatctgac cacagccgca accacactgg gcattaccgc tgaagaactt
     6241 caggccgcat tgggcaaccc cgctcaagga aaacctgact tggctgccgc agccgccaca
     6301 ctcggcgtca ctgaagatgc gctgaaacag gccattcaac aggccatgcc cgccaattgc
     6361 aacaccatgg gcggcggagc gggcaacggt cagcagcccc cgcaggaagc ctacgatgcc
     6421 tgcatcggat taagcgaagg cgcagcctgc accgtcctca ctccaaatgg aagcctgagc
     6481 ggcacctgca ccctgccgcc tgtcaatgcc tccacccagt tggcatgtgc ccccatcaa
//
LOCUS       DAOQLC010000181         6497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5006_length_6497_cov_24.148556, whole genome shotgun sequence.
ACCESSION   DAOQLC010000181 DAOQLC010000000
VERSION     DAOQLC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5006_length_6497_cov_24.148556"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PL000_19570"
     CDS             complement(<1..185)
                     /locus_tag="PL000_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91138.1"
                     /translation="MKVNFFATLRDIAGGKTVEFDVDHGITAQKLLDTIVEKYPLMKK
                     ELIHPDGHMYGHVHFFI"
     gene            complement(202..507)
                     /locus_tag="PL000_19575"
     CDS             complement(202..507)
                     /locus_tag="PL000_19575"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNC91139.1"
                     /translation="MQLRFYANMRTTVGMSSLEIGDEQIASFRGLLKVLLEKYPDTAF
                     HLLNTQGDLRPDVPVYVDGRNPRLTPLGIDTPLNPDSVVSFFSPISSGKINVEVLRD"
     gene            complement(629..1897)
                     /locus_tag="PL000_19580"
     CDS             complement(629..1897)
                     /locus_tag="PL000_19580"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC91140.1"
                     /translation="MAVLSTKTTTAWAEVDEHGRLIIPPEVAQQYGLNPGSKVRLDEG
                     TNFVRMHRPVTHLTKVYIEPTVACNLDCITCFRNAWDQPIGRMTEETFESIFNGLKKM
                     DPIPNVYFGGIGEPLFHPKTVEWIRRVKELGVKVELITNGTILTEKKSRELIDAGLDV
                     LWVSIDGATPESFTDVRMGAELPLILENLRRLFKMRKGGHFPQPEIGVAFVAMKRNIN
                     DLPKIIKMGHTFGARYFSVSNVQPATAEMQTDRLYDRTIRNIAYLPSPVLPKLSLPKM
                     DFNEETQAALTEAFNSGCNVSFAGNNWGGANDVCNFVESGTMSIAWTGEVSPCWPLMH
                     THMSYLHGKPRLSKKHVIGDVRKQTLEEIWLNPDYVAYRERLHNFVFAPCTFCGGCDL
                     SETNEEDCLGNDIAPVCGGCLWAQGIVQCP"
     gene            2063..3187
                     /locus_tag="PL000_19585"
     CDS             2063..3187
                     /locus_tag="PL000_19585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC91141.1"
                     /translation="MADSRVAKFAKVLVEHSARVVPGDRILLEGTTAAEPLLRELYIQ
                     ILEKGGHPHLMMAIPGLMPFSQDELFLTYAKDTQLDFVPTFYKLAYEQFEGRIRIHSA
                     TNTKGTTNIDPAKLQRRGKATSVITEAQFRRGADGSFKWVTTLYPTDGYAQDANMSLS
                     EYEDFVFGAVHANEDDPVAFWRSVEGKQQSAVDYMKGKSQVILRGPNVDLTLSVKGRT
                     FLNSFGTFNMPDGEIYTGPVEDSVNGWVKFTYPANYGGTSVEGAELIFSNGRVTTAKA
                     DKNQEFLLKTLDIDAGARTLGEFAIGTNFDIQRFTGNILFDEKIGGSFHMALGAGYPE
                     TGAKNKSAIHWDMICDMRTDSEILVDGELFYKNGQFVFDK"
     gene            3312..4151
                     /locus_tag="PL000_19590"
     CDS             3312..4151
                     /locus_tag="PL000_19590"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HNC91142.1"
                     /translation="MNINPTSRFYTLLKWFVPVAATFAFAAWMYLSPEGALGKLDAIG
                     YAVCHRIDVRSFQIDDRQLPLCARCTGEFYAAGVALLFQVFVSGKRSKLPSRGMIAVL
                     FLFFLAFGVDGSNSYLYLLKQTSSGALDKIPNLYVPNNTLRLFTGSGMGIALAAMLYP
                     VINQTLWRNLDDQPALEWKSLGGLIALVVVLNLLILTDSPIVLYPIAYISALGTLSLL
                     VIVFAILWIMIMRQDNSFETPSQLWLPFLSGLTLALLMILSIDMVRLQFTGTWSGFPG
                     MTG"
     gene            4180..4752
                     /locus_tag="PL000_19595"
     CDS             4180..4752
                     /locus_tag="PL000_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HNC91143.1"
                     /translation="MEMLLGIFSAFGLSASAGLNAYIPLLVVGVVAHYSPDTLALGKP
                     WDLLANPWILILLGVLVIIEMVADKVPAVNHINDVIQTVVRPVAGAIAFAASANVITD
                     ISPVLALACGLLMAGSVHAVKAVAVRPAVTATTGGAGNVPVSIAEDIAAFVTSLLAIL
                     LPIIVGTLLVILLALVLWWLWRRTDKAQTA"
     gene            4860..5015
                     /locus_tag="PL000_19600"
     CDS             4860..5015
                     /locus_tag="PL000_19600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91144.1"
                     /translation="MNDMPVSPEAPKKNNTPIIIGVVVVVLLCCCCAIAAGGWVYGDA
                     IVQSLGM"
     gene            complement(5039..5497)
                     /locus_tag="PL000_19605"
     CDS             complement(5039..5497)
                     /locus_tag="PL000_19605"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HNC91145.1"
                     /translation="MSGMKVLKKNLAEEVTWQYEGVVLSRAENAITLEALFNRDDMPF
                     MDIVLKRNDRFVETFYSDRWYNIFEIYDRDDGKLKGFYCNIGKPAVIEDDFVSYVDLA
                     LDLWVSTDGRQTVLDEDELEELNLDSELKQKVYAGLKELQGLFKSKNPPL"
     gene            complement(5485..>6497)
                     /locus_tag="PL000_19610"
     CDS             complement(5485..>6497)
                     /locus_tag="PL000_19610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91146.1"
                     /translation="ENSKMLEVRDIFKTYENKPLLRGISFSVAQGETICLLGASGSGK
                     STLLRMIAGLEFPESGHFLFNQIDLAQTPPHLRDFGLVFQDYGLFPHLNIFDNVAFGL
                     KMRNIPAEAIKPRVSEMLELVNLTGFETRRVTDLSGGEQQRVALARALAPSPRLLMFD
                     EPLGALDKSLKDDLLKQIRNILHQTKIPAIYVTHDQDEAFTIADRILLLHDGEIIRDG
                     TPSEVWNHPNSVHAARMLGVGNVIEGKIQKADGTKWKVESGIGVFEIACKHNHSAGEK
                     VSLLIRQSGEGEEIKLTVEDVLFDRDQFQVKTRGGLVIHMKDAPRVGENIRVRVKVEC
                     LA"
BASE COUNT         1539 a         1582 c         1585 g         1791 t
ORIGIN      
        1 ttgataaaaa agtggacatg cccatacata tgcccgtctg gatggatcaa ttcctttttc
       61 atgagcgggt atttttcgac aatcgtatcc aataattttt gggcggtaat gccgtggtcc
      121 acatcgaatt ccacggtttt tccgccagca atatcacgca gggtggcaaa gaaattcact
      181 ttcatgagat catctccttt attaatcgcg tagaacttcc acgtttatct taccactgga
      241 aatgggagaa aaaaagctga cgacagaatc aggattgagg ggagtatcaa ttccgagcgg
      301 agtcagacgc gggtttcttc catccacata gacaggcaca tcaggccgca agtcaccttg
      361 agtgttgagc aggtgaaacg ctgtatcggg gtatttttcc agcaatacct tcaatagccc
      421 acgaaatgaa gcaatctgct catctccgat ctcaagcgaa gacattccaa cggtcgtgcg
      481 catattggcg taaaaacgta gctgcatttt tgcttccttt ttcaaatttt atcatctttg
      541 tttttaggat gttaacgtat gttcatgata aaaaatcacc ctgaattttt tcagggtgac
      601 atggttttga tttatgaaga agcttgtttc atgggcactg cacaattccc tgcgcccaca
      661 aacacccacc acagacaggg gcgatgtcat tgccgaggca gtcctcttcg ttggtctcgg
      721 aaaggtcgca gccgccgcag aaggtacaag gcgcaaagac aaagttatgc aggcgttcac
      781 gataggctac ataatcgggg tttagccaga tctcttcgag ggtttgtttg cgcacgtcac
      841 cgatgacatg ttttttagag aggcgcggtt tgccatgcag gtagctcatg tgggtgtgca
      901 tcaatggcca gcaggggctg acctcacccg tccatgcaat ggacatggtt ccgctttcaa
      961 caaagttgca gacatcattg gcaccgcccc aattgttgcc cgcaaaactg acattgcagc
     1021 cactgttgaa ggcttcagta agggccgcct gggtttcttc gttgaagtcc attttgggca
     1081 ggctgagttt tggcagaaca ggggagggca ggtacgcgat attgcggatg gtgcgatcat
     1141 acaaacggtc ggtttgcatt tcagctgttg caggctgaac attgctgaca gagaaataac
     1201 gggcgccgaa ggtgtgtccc attttgatga tcttcggcag gtcgttaata ttgcgtttca
     1261 tggcaacaaa ggccacgcca atttcaggct gggggaaatg cccgcctttg cgcattttga
     1321 atagtcggcg caggttttcg aggatgaggg gcaattccgc gcccatgcgc acatcggtga
     1381 aactttcggg agtggcgccg tcaatggata cccacagaac atccagaccg gcatcgatca
     1441 attcgcgtga cttcttttca gtgaggatgg tgccattggt gataagctca accttgacgc
     1501 ccaattcctt gacgcgccga atccattcga ctgtttttgg gtggaagagt ggttctccaa
     1561 tgccgccaaa atatacattg gggatcggat ccattttctt taacccgttg aagatacttt
     1621 cgaaagtttc ctcggtcatg cgaccgatgg gctgatccca tgcattgcgg aagcaggtga
     1681 tgcagtcgag attacatgcg acggttggtt caatatagac cttggtgagg tgtgtcaccg
     1741 ggcgatgcat gcggacaaaa tttgtacctt cgtcgaggcg gaccttcgag ccgggattca
     1801 gcccatattg ctgggcaact tcaggcggaa tgatgaggcg tccgtgttcg tccacttctg
     1861 cccaagctgt ggtagttttt gttgatagaa cggccatgct ttctccttac aaataaacca
     1921 attaccacct atcatatcca tttttgcaaa aaattcatgt gacaaaagtt acattttcca
     1981 tattacgaag tattttgccc ttctgcaaga gtggaaataa tgaggtgata taatttcacc
     2041 attccatttt aggagcattt cagtggctga ttctcgtgtt gcaaaatttg caaaagtttt
     2101 agtggaacat tctgcacgtg ttgtgcccgg cgaccgtatt ttgctcgaag ggacaacagc
     2161 ggcagagccg ttgctgcggg agttatatat tcagattctg gaaaaaggcg gtcaccctca
     2221 tttaatgatg gcgattcccg ggttgatgcc tttcagtcag gatgaattgt ttttgaccta
     2281 tgccaaggat acgcaacttg attttgtgcc gacattttat aaattggcat atgaacaatt
     2341 cgaaggacgt atccgtattc attcggctac aaatacaaaa gggacaacga acattgaccc
     2401 cgccaaactt caacggcgcg gcaaggccac atctgtgatc acggaagcgc agttccgccg
     2461 tggggcagat gggtctttca aatgggtgac caccctctac ccgaccgatg gttatgccca
     2521 agatgccaat atgagcctgt ctgagtatga agactttgtg tttggtgctg tacatgccaa
     2581 tgaagatgat cccgttgcat tctggcgaag tgtggaaggc aagcagcaat ctgcagtgga
     2641 ctatatgaag ggcaagagtc aagtaatttt gcgcggcccc aatgtagacc tgaccctttc
     2701 cgtaaaaggg cgcactttcc tgaattcttt cggtacattt aacatgcctg acggtgagat
     2761 ttataccggt cccgtggaag actctgtgaa tggttgggtg aagttcactt atccagccaa
     2821 ttacggcggc accagtgtgg aaggcgctga gttgatcttc tccaatgggc gtgtcactac
     2881 ggccaaggct gataagaatc aggagttttt gctgaagaca cttgatattg atgcgggggc
     2941 ccgtacactg ggtgaatttg ccatcggcac caattttgat attcaaagat ttaccggcaa
     3001 cattctgttc gatgaaaaga tcggcggttc cttccatatg gcacttgggg caggttaccc
     3061 ggaaacgggt gcaaagaata agagcgcgat tcactgggac atgatctgcg atatgcgcac
     3121 tgattcggaa attcttgtgg atggtgagtt gttctacaag aatggccagt ttgtgttcga
     3181 taaatagttt taacgataat caagctgtat atttgaaggt tgaggaattt cctcaacctt
     3241 cttttgtttg ttctacatct gaaattttcg ctttcttgcc ttcccgtctt attccccata
     3301 taatgtgaac gatgaatata aatccaacgt cacgttttta tacattgtta aagtggttcg
     3361 tgcctgttgc ggcaactttt gctttcgcgg catggatgta tctttcccct gaaggcgcac
     3421 ttggaaaact ggacgccatt ggttatgcgg tctgtcatcg cattgatgtg cgctccttcc
     3481 agatcgatga ccgccagctt ccgctgtgcg cgcgttgtac gggtgaattt tatgcggcgg
     3541 gtgttgcgct gctgtttcag gtctttgtca gcggtaaacg cagcaaactg ccttcccgcg
     3601 gcatgatcgc tgttctgttc ctgttcttcc ttgcctttgg tgtggacggg agcaattcgt
     3661 acctgtatct gcttaaacaa acttcatcag gtgcgttgga taaaattcca aacctgtatg
     3721 tccccaacaa tactttgcgc ctttttacag gcagcggcat ggggatcgct ctcgcagcca
     3781 tgttgtaccc tgtcattaac cagactttgt ggcgtaacct ggatgatcag cccgctctcg
     3841 aatggaaatc actgggcggc ctgatcgcac tggtcgttgt cctcaatctg ttgattctta
     3901 cagacagccc cattgttttg tacccgatcg cctacatcag tgccttgggt accctttctc
     3961 tgttggtgat cgtctttgcc attttgtgga tcatgatcat gagacaggat aacagctttg
     4021 aaacccccag ccagctctgg ctgccttttc tttccggtct gacccttgct ttgttgatga
     4081 tcctcagcat tgatatggtc cgcctgcaat ttacaggtac gtggagtggc ttccccggga
     4141 tgacgggata gatggcgtga aaaaataaag gagagaaaaa tggaaatgtt gctcggtata
     4201 ttttctgctt ttggcctttc cgccagtgcg gggctcaatg cctatattcc gcttttggtg
     4261 gtgggggtgg tggctcatta ctcccctgat acgcttgccc tcggcaaacc ctgggacctg
     4321 ctcgccaacc catggattct cattctgttg ggggttctgg tcattattga aatggtggcc
     4381 gataaagttc ccgcagtcaa ccacatcaac gacgtcatcc aaaccgtcgt ccgccctgtg
     4441 gcgggggcca ttgcttttgc cgccagtgca aacgtcatca ccgacatcag ccccgtactt
     4501 gcgctggctt gtggtctgct catggcaggc agtgtccatg cggttaaggc ggtggccgtg
     4561 cgtccggcgg ttacagccac tacaggcggg gcagggaacg tccccgtttc cattgctgag
     4621 gatatcgccg cgttcgtcac atccctgctg gcaattcttc tcccgatcat cgtcggcacc
     4681 ctgctggtca ttttgctggc actagtactt tggtggctct ggcgccgcac agacaaggcg
     4741 caaactgcct gatataatcg ggccaatcaa attcgtactg ctgacaaaaa aagcaaaaat
     4801 ctgttcagca cgcgcaaaat tcgtgtcgcg cgattcaatt atcaaaagga gtagaaatca
     4861 tgaatgacat gcctgtttcc cctgaagcgc cgaagaaaaa taacaccccc attattatcg
     4921 gcgtagttgt tgtagttttg ctttgctgct gctgtgccat tgcggcaggc ggctgggtct
     4981 acggcgacgc cattgtccaa tcgcttggaa tgtaattgac tgtaaaaaat cctccactct
     5041 aaagtggagg attttttgat ttaaacaacc cctgtaattc tttcagccca gcatacactt
     5101 tctgctttaa ttcagaatcc aaattcaact cttccaattc gtcttcatcc aaaaccgttt
     5161 gcctgccatc tgttgaaacc cacaggtcca gtgcaaggtc tacatatgaa acgaaatcat
     5221 cttcgatcac ggcgggtttg ccgatattgc agtagaaacc cttcagtttt ccatcatcgc
     5281 ggtcataaat ttcaaagatg ttgtaccagc ggtcagagta aaaggtttcc acaaaacggt
     5341 cattgcgttt cagcacaata tccatgaacg gcatatcgtc gcggttgaac aacgcctcca
     5401 gcgtaatggc attttctgcg cggctcaaca ccaccccttc atattgccac gtcacttctt
     5461 ctgcgaggtt tttcttcaaa actttcatgc cagacattct accttaaccc gcacacggat
     5521 gttttcaccg actcgcggag cgtctttcat gtggatgaca agcccgcctc ttgtcttaac
     5581 ctgaaactga tcccgatcaa aaagcacatc ctccactgtg agttttatct cctcaccttc
     5641 acccgactgc ctgatcagca gcgaaacttt ttctcccgct gaatgattgt gcttgcaggc
     5701 tatttcaaaa acaccaatcc cactttccac tttccatttt gtaccatctg ccttctgtat
     5761 ttttccttca ataacgttcc ccacccccaa catccgcgcc gcgtggacag agtttggatg
     5821 attccacact tcactgggcg taccgtcgcg gatgatctcg ccgtcgtgca aaagcaaaat
     5881 gcggtctgca atggtgaagg cttcatcttg atcgtgtgtg acataaatgg cgggaatttt
     5941 tgtttgatgc aggatattgc gaatctgctt cagcagatca tctttcaaag atttatccaa
     6001 agccccaagt ggctcatcga acatcaacaa gcgcggactt ggcgcaaggg cgcgggcgag
     6061 tgccacacgc tgctgctcgc cgcctgaaag gtcggtcacc ctgcgggttt caaagcctgt
     6121 caaattgacc aactccaaca tttcagagac tcttggtttt attgcttcgg cgggaatgtt
     6181 cctcattttt aacccaaacg ccacgttgtc aaaaatgttc aggtgaggaa aaagcccata
     6241 gtcttgaaag accaagccaa agtcgcggag gtgaggcggt gtttgggcga ggtcaatctg
     6301 attaaacagg aaatgtccgc tttcaggaaa ctccaaacca gcgatcatcc gcaagagagt
     6361 ggattttcca ctgcccgatg cgccaagcag gcaaatggtt tcgccctgtg ccacggaaaa
     6421 tgaaatgccg cgcagaagcg gtttgttttc gtaggttttg aaaatgtcgc ggacttcgag
     6481 cattttagaa ttctcca
//
LOCUS       DAOQLC010000182         6460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5048_length_6460_cov_26.896487, whole genome shotgun sequence.
ACCESSION   DAOQLC010000182 DAOQLC010000000
VERSION     DAOQLC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_6460_cov_26.896487"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PL000_19615"
     CDS             complement(<1..724)
                     /locus_tag="PL000_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNC91147.1"
                     /translation="MQDLFKSRDVLKVGKKEYVYFRLDALEKAGLVKLSKLPYSIRIV
                     LEAALRQCNDKEITQEDVKNIAAWSPKGARPGIPFLPGRVVMQDFTGVPAVVDLAAMR
                     AAVARLGGDPKKINPLVPVDLVIDHSVQIDFFATADALNRNTEMEFLRNKERYEFLKW
                     GQKAFSNFRVVPPMTGIVHQVNLENLAEVVMTKVDGNDVIAFPDTLVGTDSHTTMING
                     LGVVGWGVGGIEAEAVMLGQPMD"
     gene            complement(939..1979)
                     /gene="add"
                     /locus_tag="PL000_19620"
     CDS             complement(939..1979)
                     /gene="add"
                     /locus_tag="PL000_19620"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HNC91148.1"
                     /translation="MLSHQTVPLNQYFSLPKVELHRHLEGSLRLTTMLDIARQHGVTV
                     PASMFNLSGLVQVQDQDPMTFTNFLEKFKTLRLFYKSPDVIHRVTREAVEDAAKDNIR
                     YLELRFTPVALSRAEGFPLHDVMDWVLTSAQEAAKKSNIKVGLIASVNRHESPELAEQ
                     VVWLAAEHMKDGMVGIDLAGNEAEFKSAPFRDIFKEAKQSGLHVTIHAGEWGPAENVR
                     DALVNFDAERIGHGVRVMEDENIVALAKERGVTFEVCVTSNYQSGVVKSLPQHPLPRM
                     MAAGLHVTVNTDDPSVSRITLSHEYQHVCEDMKVSFDALRQSVLHAVQASFTTQDEKT
                     KLVAALKKEMKM"
     gene            complement(1993..6021)
                     /locus_tag="PL000_19625"
     CDS             complement(1993..6021)
                     /locus_tag="PL000_19625"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91149.1"
                     /translation="MLKNFVKLFSGDPTKKTADKFGDLVTKVNALEAQFEALSDEALR
                     AKTDEFKARLAQALQGLTPPPVGEKGSEEGREYRKFEQDALNELMPEAFAAVREASKR
                     TIGLRHYDVQVIGGGILHGGSIAEMKTGEGKTLVATLPVYLNAIAGRGVHIITVNDYL
                     ARRDARWMAPVYNMLGLSVGVLQMAAVTENGKKAFIVDFERESPHEDQEHLRMVDRVE
                     AYRADITFGTNSEFGFDYLRDNMAMRMESRVQRGHYFAIVDEVDNVLIDEARTPLIIS
                     GPASGDLEWYGRMAQVVKQLRPEDYEVDEKNRNVSLTEIGVSRVEAILGTQLLDPDRP
                     EDLTPEQARLTGYLQQALRAQYLFHRNKDYLVQGGKVVIVDEFTGRQMPGRRWSDGLH
                     QAVEAKEGVKVEPENVTYATITLQNYFRMYEKLAGMTGTALTEAEEFNKIYKIEVAPV
                     PTNLEYQSLRSDATLVEQKAKDEDGYQYSYYTARGGDADGKIFYRRKDYPDVIYRTVE
                     AKLRAIVTEIVRYHALGRPMLVGTTSVESSDRLSNRLKAEPVRRLLQVTLVREMYLRA
                     NNMEEDGRLIEALVPFNEPIDKITPDALRKFIAPFGSNNISLDDDNNLNTLLSVLRLP
                     ETAKSRLKTIIQGGVLHQVLNARKHTEESQIIAGAGAFGAVTIATNMAGRGVDIKLGG
                     ELAEEVISAVNRVLRKAGHNDPFDLTQEERRQALLKADTSHYGIYEAEVKLFLQYFED
                     MESVKKLGGLHVIGSERHEARRIDNQLRGRAARQGDPGSSRFYLSLQDDLMRLFGGDQ
                     VSNLMTRLKVDEALPLEVRLVSNIIEGSQTRVEGANFDVRKHLLEYDDVLNEQRTQIY
                     NQRDRIFVKEDLSEDVAGMLENEVTKRVDLGLEDEEGPWKLLAWLEQVQPPFESQNGL
                     FPSYGFKLILEQIKQDDVQQSMLGIISRAIQAESDHHLRAIEALVAKTRESFDTQTSE
                     RDDAIDAYFEGLRDREDAPRAQKIAEELSALVRLPLKLNNEMTRALGDDPDSVKEDIQ
                     EMVAGQLTALSAARLIGAIQNRVGEQLPWPNPLPSDWDELEDVIVQTAREGLSHKSER
                     LTAQIARDMETLFQRESADSDAGRLRMLLTLSQGTRATFDQKTHKQVRQLFLRFSYIF
                     LAAQLLEGREAPDIVEDIMGHLETAEETLRITWGQSEFTRLNQNATRLADFGPAARIA
                     FGESRLNDAVSGLSESDRESLIDAIGRYVLNEVHRQLLLSAFSELWVEYLTKVEALRI
                     SIGLEAYAQRDPLVQYKGRASEMFQQLLEDVRGLVIGRAFAARPRRVEITPIETAEGE
                     VVSAPAETQVQMAGNKKKRKRH"
     gene            complement(6114..>6460)
                     /locus_tag="PL000_19630"
     CDS             complement(6114..>6460)
                     /locus_tag="PL000_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91150.1"
                     /translation="NKFTVEFDADMRRFFWPQQGKDSTQVTSLSVARWTPYHETAWDD
                     MFTQMVAVLQPAVNSAFDHYKQTSSLSLGDSPYSANIKSLLVQMEAQINDLSRLCYPQ
                     EVERFLFRPPRI"
BASE COUNT         1480 a         1822 c         1627 g         1531 t
ORIGIN      
        1 tgtccatcgg ctggccgagc atcacagctt cggcttcgat gccgcccaca ccccaaccca
       61 caacgccgag gccgttgatc atggtggtgt gtgagtcggt gccgacgagg gtatcgggga
      121 aggcgatgac atcattgcca tccaccttgg tcatcaccac ttcggcaagg ttctcgaggt
      181 tgacttgatg cacaataccg gtcattggcg gcaccacgcg gaaattgctg aacgcttttt
      241 gtccccattt gaggaattcg tagcgttcct tgttgcgcag gaattccatt tcggtgttgc
      301 ggttgagggc atcggcggtg gcaaagaaat cgatctgcac cgagtggtca atgacgaggt
      361 ctaccggcac gagcgggttg atcttctttg ggtcgccgcc caggcgggcc acggcagcac
      421 gcatcgcggc aaggtcaacg acagcaggca caccggtaaa atcctgcatg acgacacgtc
      481 cgggcaaaaa cggaatgcca gggcgcgcac ctttgggaga ccacgcggcg atgttcttca
      541 catcctcctg cgtgatttcc ttgtcattgc attggcggag ggcagcctcc agtacgatgc
      601 ggatggaata aggcagtttg ctgagcttta ccagcccggc cttctccagc gcatcaaggc
      661 ggaagtagac gtattccttc tttcccactt tcaatacgtc gcgggactta aacaggtctt
      721 gcataggata ctcctttggc atgaggattc tcgtgtgaca aactaagatt gtttgtcgta
      781 cgctcacacc acaatctgga agaactcaaa ggagcgcctt tgatatcctt tttactatgt
      841 ggtgggattt acatggcagc gtccctcgct acgctcggga cgattcatca caacgatcat
      901 ttgaaacctg tgcatcgcaa atatgcgcag ggaaaaacct acatcttcat ttcttttttc
      961 aatgcggcaa ccagtttcgt tttttcatcc tgcgttgtaa acgaggcctg aaccgcatgc
     1021 agaacactct gtcgcagcgc atcgaaggaa accttcatat cctcgcagac atgctggtat
     1081 tcatgcgaga gcgtgatgcg cgacacgctg ggatcatcgg tattgacggt cacatgcagg
     1141 cctgccgcca tcatacgcgg caacgggtgc tgcggcaaag acttgaccac accggactga
     1201 taattgctgg ttacgcacac ttcgaaagtc acgccgcgtt ccttcgcgag cgccacaata
     1261 ttttcatctt ccatcacgcg cacaccgtga ccgatacgct cggcatcgaa attcacaagc
     1321 gcatcgcgga cattctcagc gggtccccac tcacccgcat ggatggtgac atgcagcccg
     1381 gattgtttcg cttccttgaa aatatcgcgg aatggagcag acttgaactc ggcctcatta
     1441 ccagccaggt caatgcccac catgccatcc ttcatatgct ctgccgccag ccagaccacc
     1501 tgctcagcaa gctcggggct ttcatggcgg ttcaccgaag cgatcaaacc gaccttgata
     1561 ttacttttct tcgcggcttc ctgcgcactg gtcaacaccc aatccattac atcatgcagc
     1621 ggaaacccct cggcgcggct aagcgccaca ggtgtaaaac gaagctcaag gtagcggata
     1681 ttatccttcg ccgcatcctc caccgcctcg cgcgtaacac gatgaatgac atcgggagat
     1741 ttataaaaaa gccgcagcgt tttgaatttt tcgagaaaat tcgtaaacgt cattgggtcc
     1801 tgatcctgca cctgcaccag gccgctcaag ttaaacatgc tggcaggaac tgtcacgcca
     1861 tgctggcggg cgatatccag catggtcgtc aagcgcagag acccttcaag atgacggtgt
     1921 aactccacct ttggaaggga aaaatattga ttcagcggaa cagtttggtg ggataacaac
     1981 ttgcctcaaa aactaatgtc gtttccgttt cttcttattg ccagccattt gcacctgagt
     2041 ttcagcaggc gcagaaacca cttcaccttc agcagtttca atgggagtga tctccacacg
     2101 gcgcggtctg gccgcaaatg cacgtccgat caccaatcca cgcacatctt caagcaattg
     2161 ctggaacatt tcagatgcac ggcctttata ttgtaccagc gggtcacgct gtgcgtaagc
     2221 ctcgaggccg atggaaatac gcaacgcctc cacctttgtg agatattcca cccacaattc
     2281 ggagaacgca ctcaataaca actggcgatg gacttcatta aggacatatc ttccaatggc
     2341 atcgatgaga gactcgcggt ctgactcact gaggcctgat accgcgtcat tgagccgact
     2401 ctccccgaat gcgatccgtg cagcggggcc aaaatctgcc agacgggtgg cattctgatt
     2461 caaacgggta aattcgctct gtccccaggt aatgcgcagg gtttcctccg ccgtttcaag
     2521 atgccccatg atgtcttcca caatgtcggg agcttcgcgc ccttccagca attgcgcggc
     2581 gaggaaaata tacgagaacc gcaggaacaa ctgtcggacc tgtttgtgcg tcttttggtc
     2641 gaacgtggcc cgcgtaccct gtgaaagggt cagcaacatg cgcaacctgc ccgcatccga
     2701 atcagccgat tcgcgctgga acagagtttc catatcgcga gcgatctggg cggtaaggcg
     2761 ttcactctta tgtgaaagcc cttcgcgcgc agtttgcacg atcacatctt ccaactcgtc
     2821 ccaatccgaa ggcagcggat tcggccacgg caactgctca cccacacggt tttgaatcgc
     2881 gccaatgagg cgagcggcac tcagggcagt caactgccca gccaccatct cctgaatatc
     2941 ctctttcaca gaatcagggt catcgccaag agcgcgggtc atttcattat taagttttaa
     3001 cggcaggcgc accagagcgc tgagctcttc tgcgatcttc tgggcacgcg gagcatcttc
     3061 acggtcgcgc aggccttcga agtaggcatc aattgcatcg tctcgctcgg aggtttgggt
     3121 gtcgaaggat tcgcgtgttt tcgcaaccaa cgcctcaatg gcacgcagat gatgatcact
     3181 ctctgcctga atggcacgag agatgatccc cagcattgat tgctgaacat catcctgttt
     3241 gatctgctca aggatcaatt tgaaaccata ggaagggaac agaccatttt gtgattcgaa
     3301 aggcggctga acctgttcaa gccaggccag cagtttccac ggaccttctt catcctcaag
     3361 gccgagatcc acacgtttgg tcacttcatt ttcaagcatg cctgcaacat cttcactcag
     3421 gtcttccttc acaaagatgc ggtcgcgctg attgtagatc tgtgtgcgct gttcgttgag
     3481 cacatcatcg tattccagca aatgcttgcg gacatcgaaa ttcgcgcctt ccacacgggt
     3541 ctgcgaacct tcaatgatgt tgcttacaag gcgtacttcc agcgggaggg cttcatccac
     3601 tttaagacgg gtcatcaggt tgctgacctg gtcgccgcca aacaggcgca tcaggtcatc
     3661 ttgaagcgag aggtaaaaac gcgaagagcc agggtcaccc tgacgggcag cacgaccgcg
     3721 cagctgatta tcgatacgtc gtgcttcatg gcgctctgag ccaatgacat gcaaaccgcc
     3781 cagcttcttc acactttcca tatcttcaaa atattgcagg aaaagtttga cctcggcctc
     3841 gtaaattccg taatgtgagg tgtctgcttt caacaaagcc tgacggcgtt cttcctgtgt
     3901 gagatcgaag ggatcgttgt gccccgcctt gcgcaacacc cgattcacag ccgaaatgac
     3961 ctcttcagcc aattcgccgc cgagtttaat atcaacaccg cgacccgcca tgttggtggc
     4021 gatggtcact gcgccaaaag cacctgcacc agcgatgatc tgcgattctt ccgtgtgttt
     4081 gcgcgcattc aatacttgat gtagcacacc gccctgaatg atcgtcttca gtcggctctt
     4141 cgctgtttca ggcaggcgca aaactgacaa caaggtgttc aggttgttat catcatcaag
     4201 gctgatattg ttcgaaccga atggcgcaat aaatttgcgc aatgcgtctg gagtgatctt
     4261 gtcaattggc tcgttgaacg gcaccagcgc ctcgatcagg cggccatcct cttccatatt
     4321 attggcacgc aggtacattt cacggacaag agtcacctgc agtaaacggc gaacaggctc
     4381 ggccttgagt cgattcgaga gtcggtccga agattccacc gaagtggtac ccaccagcat
     4441 cgggcgtccg agcgcatgat agcggacgat ctccgtcaca atggcacgca gctttgcctc
     4501 gaccgtgcgg taaatgacat cgggataatc ttttctgcgg tagaagatct tcccatcggc
     4561 atcgccgccc ctggcggtgt aataggaata ttgataacca tcctcgtcct tggccttttg
     4621 ctccacaagc gtggcatcag aacgcaggga ctgatattca aggttggtgg gcacgggggc
     4681 cacttcgatc ttgtagatct tgttgaattc ctccgcttcg gtgagcgcag taccggtcat
     4741 gcctgccagc ttttcataca tgcggaagta gttttgcagg gtgatggtgg cgtaggttac
     4801 attttcaggc tcaaccttca caccttcctt ggcttccact gcttgatgca ggccgtcgct
     4861 ccaacggcgt ccgggcatct gccgcccggt gaattcatct acaatgacga ccttgccgcc
     4921 ctgcacaaga taatccttgt tacgatggaa aagatattgg gcacgcaatg cctgctggag
     4981 ataccctgtc agacgcgcct gctccggggt gaggtcttcg ggtcggtctg gatcgagaag
     5041 ctgggtgccc aaaatagcct ctacacggct gacaccgatc tcggtgagag agacattgcg
     5101 attcttttca tccacttcat aatcttcggg gcgtaattgc ttcaccacct gtgccatgcg
     5161 tccgtaccat tcaagatcgc ctgaagcggg cccggagatg atcaacggtg tacgcgcttc
     5221 atcaatcagc acgttatcca cttcatccac gatcgcaaag tagtgaccac gctgtacgcg
     5281 cgattccatg cgcattgcca tgttgtcgcg cagataatcg aaaccaaatt cagagttcgt
     5341 accaaaggtg atatcggcgc gatacgcctc gacccgatcc accatgcgga gatgttcctg
     5401 gtcttcatgc ggcgactcac gttcgaaatc cacaatgaag gctttctttc cattctcggt
     5461 gacggcggcc atttgtaaaa cgcccacact caagccaagc atgttgtaga ccggagccat
     5521 ccagcgggca tcacgacgcg ccagataatc attgaccgta atgatatgga ccccgcgccc
     5581 cgcgatcgca ttgagataaa caggcagggt ggccacaagg gttttgcctt cacccgtctt
     5641 catttcagca atggaaccgc cgtgcaaaat tccgccgccg atgacctgca catcgtagtg
     5701 acgcaggcca atggtccgtt tggatgcttc gcgcaccgcc gcaaatgcct caggcatgag
     5761 ctcgttcaac gcatcctgct caaacttgcg atactccctg ccctcttcgc ttcccttctc
     5821 gccaacgggc ggaggggtca gtccctgcaa cgcctgcgcc aggcgcgcct tgaattcatc
     5881 cgtctttgca cgcaaggcct cgtcactcaa agcctcaaat tgtgcttcaa gcgcattcac
     5941 ctttgtcaca aggtcgccaa atttatcagc cgttttcttt gtggggtcgc cactgaatag
     6001 ttttacgaag ttcttaagca taatatccct ttcagggaag ggattatagc attcggagag
     6061 ggtatggggg atttgagttt tattaatgtt tggggggcag tgctattcca ttttcatatg
     6121 cgcggcgggc ggaacaggaa acgctcaact tcctgcgggt agcacagacg ggataagtca
     6181 ttgatctgcg cctccatttg caccaacaga cttttgatat ttgccgaata tggagagtcg
     6241 cccagtgaaa gcgagctggt ctgcttgtaa tggtcaaaag ccgaattcac ggcgggctga
     6301 agtactgcaa ccatttgcgt gaacatatca tcccatgcag tttcatgata gggcgtccaa
     6361 cgcgccacac tcaaagacgt gacctgggtc gaatctttgc cttgttgcgg ccagaaaaac
     6421 ctgcgcatgt ccgcatcaaa ttccacagtg aacttgttaa
//
LOCUS       DAOQLC010000183         6403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5117_length_6403_cov_26.150126, whole genome shotgun sequence.
ACCESSION   DAOQLC010000183 DAOQLC010000000
VERSION     DAOQLC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5117_length_6403_cov_26.150126"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..654)
                     /locus_tag="PL000_19635"
     CDS             complement(10..654)
                     /locus_tag="PL000_19635"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC91151.1"
                     /translation="MSAEETLAQLAVKISTCTNCALHESRKKAVTGDGPADAEIMFIG
                     EGPGFHENEQGHPFVGASGKFLDQLLAQAGVTRAEVFIANVVKCRPPGNRDPLPEELA
                     ACDEYLEAQIQTINPSIIVTLGRFSMNKFIPGAKISAIHGQMRKVGDRFVIPMFHPAA
                     ALHQAALKPSILADFANLPDQLNEARAALGRKVVAQKKEAAAPKNEKPRQLSLF"
     gene            complement(644..1162)
                     /gene="rimI"
                     /locus_tag="PL000_19640"
     CDS             complement(644..1162)
                     /gene="rimI"
                     /locus_tag="PL000_19640"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNC91152.1"
                     /translation="MNVLSPDDGSGLMKIRKMTVDDLEQVIAIDQVSFGLPWPPRSFS
                     FELTDNPPSRCWVVEMNGRVVAMLVAWLIVDEIHIATFATHPDHRRQGIGGRLLYHTL
                     RTAREEGAITSFLEVRAGNESALALYRKFGYVESGLRKGYYSDNHEDAVLMTLGPADY
                     ERLLLHGEADER"
     gene            complement(1183..1854)
                     /gene="tsaB"
                     /locus_tag="PL000_19645"
     CDS             complement(1183..1854)
                     /gene="tsaB"
                     /locus_tag="PL000_19645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNC91153.1"
                     /translation="MLIAVDTSTAQVGLALYDGNQVLGELTWTTRQHHTTELAPALTG
                     LLSRSGVSMDMVSALGVAIGPGSFTSLRVGLALVKGLALARRLPVIGISTLDVIAAAQ
                     PASKHPLLAVIQAGRKRIAFSEYKSLKNEWQVQGPVRSGTVDELADSIESPTVVAGEF
                     SPDDRSRLSKKRAKILLASPAYCVRRPSLLAELAWERWQKNEVDDAASLAPIYLHVEG
                     TPIPS"
     gene            complement(1854..2351)
                     /gene="tsaE"
                     /locus_tag="PL000_19650"
     CDS             complement(1854..2351)
                     /gene="tsaE"
                     /locus_tag="PL000_19650"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNC91154.1"
                     /translation="MPILDSHTIEFFSRSPEQTRRIGMRTGAGLKAGDVICLQGNLGA
                     GKTTFVQGLAQGWGSLDSVSSPTFILVNQYRRANGDRLFHLDAYRIESMPEGEELDLD
                     SMLADGALVIEWPERLGDLIPKERMWINLEHMAEEHREMRFNANGKRYDDLLDEIRQS
                     IFGGA"
     gene            complement(2478..3716)
                     /locus_tag="PL000_19655"
     CDS             complement(2478..3716)
                     /locus_tag="PL000_19655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC91155.1"
                     /translation="MAETEARSVPKVGSLRIGLFTDTYAPQVNGVSISLQLISDGLRR
                     RGHQVTIFAPRFPGYEDNETNVMRLPSLKYLNNPPIYVAVLGTPRSTWSLTRKHFDVL
                     HAHSPASVGLLAYLTASTKRLPLIYTYHTSITDYTHYVKFIGGSSIIKHAASWFSTTS
                     TNLGDQIVVPSPKFHRLLLEQKVKRPIHIIPNGINLDGFKSAKNPGSFRNRLGMQPDA
                     PILLSVGRIDPEKRLDFLIDAFDRIADHVPNARLVFAGDGSARKKLEEHAASVRSKDR
                     IHFLGMVNRADLPDLLHDAAVFLSASTTEVHPISVIEAIAAGLPVLAVQDEAFEGMVI
                     DGENGHQTLLDVNVYSDTLLSLLKDPERLIRYGKRSAELSEKYSIEGQVRALENLYIE
                     SILQNWRGSLVSRISARLKL"
     gene            complement(3815..4027)
                     /locus_tag="PL000_19660"
     CDS             complement(3815..4027)
                     /locus_tag="PL000_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HNC91156.1"
                     /translation="MLPDLQLKDHLRLRKPHPCGSYEWTVVRLGADIGLECRGCQHRV
                     MLTRRELAKRLKTNLTKHEEKKEEGK"
     gene            4209..5171
                     /locus_tag="PL000_19665"
     CDS             4209..5171
                     /locus_tag="PL000_19665"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC91157.1"
                     /translation="MNDELKIKFWGVRGSYPTPGADTIRYGGNTSCVEVSAGGRTIIL
                     DAGTGIIPLGRELAGQKRPLELLLLFSHLHHDHTQGFPFFIPAYIPNTRLHIFGPGGT
                     PETVKHVLEHNQSAETFPLSLRDMAAAKQIESVRESQVIVWDEENVRVVGSASGLSPE
                     AVVIRIHKSYAHPGGVYVYRISWRGKSVVYATDTEGYVGMDRKLAAFARDADVLIHDA
                     QYSEEHYRGMLAGFPSTQGYGHSTALMAAEVAASAETGELILFHHDPAYSDDWVAAQE
                     AAAKKLFPDSCAAYEGLEIRLKPEQAGMVLTGPTQARRGNVSPR"
     gene            5194..5892
                     /locus_tag="PL000_19670"
     CDS             5194..5892
                     /locus_tag="PL000_19670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC91158.1"
                     /translation="MTEIKRDDTRNILSDIKLFEGLSSAQLDWVAKHAHRRIFTAGTN
                     VLTIEQPGEAVFIILHGTVKIHIEQGERDVIISVLGSGDILGEMSLIDSVGRSASAIT
                     LENSLMLWMDKTTFYHILDNFPPVARNLVKILTARVRLSDQVIQSLATLDVNGRVARQ
                     LLAFAERYGHEIADGVQVHIVLTQGDLADLVGASRKRVNQAMVFFKEQGLVSTDADGR
                     FVIHNKAGLMHFCN"
     gene            5994..>6403
                     /locus_tag="PL000_19675"
     CDS             5994..>6403
                     /locus_tag="PL000_19675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC91159.1"
                     /translation="MSDYKFFHPTEVRYGDLDPQGHVNNAKYLTYFEQARIYYLIKLG
                     LFNKDQSFMEVGVIIADIHITYHSTTHYGDDIKVGVKITRIGTKSITVEQCVMDAKTG
                     KLMASGTVVMVTFDYEGLKTIPVPEEWKQKISAF"
BASE COUNT         1546 a         1730 c         1672 g         1455 t
ORIGIN      
        1 atctgttgac taaaacagac tcaactgcct tggcttttca ttttttggcg cagccgcttc
       61 ctttttttgt gcaacaacct ttcggcccaa tgcagcgcgg gcttcgttca attgatcggg
      121 caggttggca aaatctgcca gaatggacgg tttgagcgct gcctgatgca aagccgccgc
      181 agggtggaac atgggaatga caaagcggtc gcccactttg cgcatctgcc cgtggatggc
      241 actgatcttc gcgccgggga tgaacttgtt catcgaaaag cggcccagcg tcacaatgat
      301 actcggattg atggtttgaa tttgcgcttc aagatattcg tcacacgcgg ccaattcctc
      361 gggcaatggg tcgcggttgc cgggcgggcg gcacttgacc acattggcaa tgaaaacctc
      421 cgcacgggtc acccctgcct gtgccagcaa ctggtctaag aactttccag aggcacccac
      481 aaagggatgt ccctgctcgt tctcatgaaa gcctggtcct tcgccgatga acatgatctc
      541 cgcatccgcc gggccatcgc ccgtcacggc ttttttgcgt gattcatgca gcgcacaatt
      601 cgtgcaagtg gatattttca cggccagttg ggcaagggtc tcttcagcgc tcatccgctt
      661 ctccatggag cagtaatctt tcataatcgg cggggccgag ggtcatcaac accgcatctt
      721 catgattatc cgagtaatac cccttgcgca ggccgctctc cacgtaccca aacttgcggt
      781 acaaagccag agccgattca ttcccagccc gcacctccaa aaaagaggtg atcgcgcctt
      841 cctctcttgc cgttctcagc gtgtgataca gcaatcttcc cccaattccc tgcctgcgat
      901 gatcagggtg ggttgcaaag gtcgcaatgt ggatctcatc cacgatcagc catgcaacca
      961 gcattgcaac cacacgacca ttcatctcta ccacccaaca acgggagggc gggttatcag
     1021 tcaattcaaa agaaaacgaa cgcgggggcc acggcaaacc aaacgaaacc tgatcaatgg
     1081 caatgacctg ctccaggtca tccacagtca tcttgcgtat cttcatcagc cccgacccat
     1141 cgtcgggaga aaggacgttc atgctcaaaa tttcccgatg gactacgacg gaatcggtgt
     1201 cccttccaca tgcaggtaga tcggcgcaag cgaagcggca tcatccactt catttttttg
     1261 ccagcgttcc caggccaact cggcaagaag cgatggtcgc cgcacacaat atgcaggtga
     1321 agccagcagg attttggcgc gtttcttcga aagacggctg cggtcatcgg gggaaaattc
     1381 acccgccaca acagtgggac tctcgatcga atcagccaac tcatccaccg tcccgcttct
     1441 cactggacct tgaacctgcc actcgttctt caagctttta tactcgctga aagcgatccg
     1501 cttccgcccg gcttgaatca cagccagcaa tggatgcttg ctcgcaggct gtgccgccgc
     1561 aatcacatcc agcgtggaga tgccgatcac aggcaggcgc cgtgccagag caagtccctt
     1621 caccagagca agccccaccc gcaggctggt gaaggagccc gggccgatgg ccacccccaa
     1681 agcgctgacc atatccatcg aaacaccgga gcggctcaaa agtccagtaa gagccggggc
     1741 caattcagtg gtgtgatgct gacgggtcgt ccatgtcaat tcaccaagca cctggtttcc
     1801 atcatataaa gccagcccaa cctgggccgt ggaagtatca acagcaatca gcattacgcc
     1861 cctccaaaaa tggattgacg gatttcatcc aacaggtcat cgtagcgttt gccgttcgca
     1921 ttgaaacgca tttcacggtg ctcctccgcc atgtgttcga ggttgatcca catgcgttct
     1981 ttggggatca ggtcgcccaa acgttcgggc cattcaatga ccagggcgcc atccgccaac
     2041 atggaatcga gatcgagttc ctcgccttct ggcatcgact caatgcgata tgcatccaga
     2101 tgaaagaggc ggtcgccgtt tgcgcggcga tattggttta cgagaataaa tgtcgggctg
     2161 gaaaccgaat cgagtgaccc ccatccctgc gcaagaccct gcacgaacgt ggtctttcct
     2221 gcgccgaggt taccttgtaa acagatcaca tcaccagcct tcaaacccgc ccctgtccgc
     2281 attccaatgc ggcgtgtttg ttccgggctt cggctgaaaa attcaatggt atgagaatca
     2341 agaatgggca tcgcgggaga attatactcc ctgaaaatcc acgcgagaaa aaataaccca
     2401 ccaggggaca aaagtgtcac gaaggataaa ccctgccatt tccagcatct cttcgtgaca
     2461 cttttgggta aaacttttta taacttcaac cttgccgaaa tacgcgaaac caggctgccg
     2521 cgccagtttt gcaaaataga ctcaatataa aggttttcca aagcacgcac ctgcccctca
     2581 atggaatatt tctcgctgag ttctgcagaa cgttttccat agcggattaa tctttcgggg
     2641 tctttcagca ggctcaacaa cgtatcgctg tacacattca catccagcaa ggtctggtga
     2701 ccattctctc catcgatcac catgccttca aacgcttcat cctgcacagc cagcacaggc
     2761 agcccggccg caatggcctc gatcaccgaa atgggatgca cctccgtggt ggaagcggaa
     2821 aggaaaaccg cggcatcgtg cagcaggtcc ggcagatcgg cacggttgac catgccaagg
     2881 aagtgaatgc ggtctttcga cctgaccgaa gcagcatgct cttccaattt tttgcgcgcg
     2941 cttccatccc cggcaaaaac aaggcgggca ttgggaacat ggtccgcaat gcgatcgaag
     3001 gcgtcaatga ggaaatccag acgcttttcc gggtcaatgc gcccaaccga aagcaaaatc
     3061 ggcgcatcgg gttgcatacc caatcggttt ctaaaactgc cggggttttt ggcagatttg
     3121 aaaccatcca gattaatgcc attcggaatg atatgaatcg gccgtttcac cttctgttcc
     3181 aacagcaggc ggtggaactt tggtgaaggc acaacgatct gatcgcccag gttggtggag
     3241 gtggtgctga accaggaagc cgcgtgttta atgatgcttg acccgccaat gaattttacg
     3301 taatgggtgt aatcggtaat ggatgtatgg taggtgtaga tcagcggaag ccgttttgtg
     3361 gaggccgtaa ggtaggccag caggcccaca ctggccgggc tgtgcgcgtg caggacatcg
     3421 aaatgcttgc gcgtcagcga ccaggtggaa cggggggttc caagcaccgc cacatagatt
     3481 ggcgggttgt tcaaatattt cagcgagggc aggcgcatga cgttggtttc attatcttca
     3541 taccccggga aacgcggcgc aaaaatcgtc acctgatggc cgcggcggcg cagcccatca
     3601 gagatcaatt gcagggaaat ggaaacccca ttaacttgcg gcgcatacgt atctgtgaac
     3661 aaaccaatgc gaagtgaccc cacctttgga actgatcgtg cttcggtttc tgccatgttt
     3721 ttattttctc ctgagatcct tttatgaagc aaacatcccg caggcttttg gattcaatgg
     3781 tcaattcatc caaacctgcg ggatcgggca cctgctactt cccttcctct tttttctctt
     3841 catgcttcgt caaattggtc ttcaatcgtt tcgccaattc acgacgggtc agcattacgc
     3901 ggtgctgaca gcctctgcat tccaaaccga tatccgcccc aaggcgcacc actgtccatt
     3961 cataggaacc gcatgggtgc ggcttgcgta gtcggaggtg atctttcaat tgcaagtcag
     4021 gtagcatgga tggaattata ccgccacggc ttgcatcccc gctttgcatg ggttaatggt
     4081 ttgtaaacaa tatggtcagc gtgtgacata tttcccagcc tgcatgcgaa aactttcctt
     4141 gagaaaaaag ccataaagac gataataaac ttgcaagtac aaaatatgga atcagaacgg
     4201 aggttgccat gaacgatgaa ctgaagatca aattctgggg tgtgcgcgga agttacccca
     4261 cccccggcgc ggatacgatc cgatatggcg gcaacacttc ctgtgtggag gtcagcgcgg
     4321 gcgggcgcac gatcattctc gatgcgggca cgggcatcat tccgctgggg cgtgaattgg
     4381 cagggcaaaa acgtccgctg gaactgctgt tgttattcag ccacctgcat cacgaccaca
     4441 cccagggatt tccatttttc atccccgctt atatcccgaa cacgcggctg cacatcttcg
     4501 gcccgggcgg cacacctgaa accgtgaagc atgtccttga acacaatcaa tcggcagaga
     4561 cgttcccgct cagcttgcgc gatatggccg ccgccaaaca gatcgagtcg gtgcgcgagt
     4621 cgcaggtcat tgtttgggat gaagaaaatg ttcgggttgt tggatcagct tcaggattaa
     4681 gtccggaggc ggttgtcatc cgcattcaca aatcgtacgc gcatcccggc ggtgtgtatg
     4741 tgtatcggat ttcgtggcgc ggtaaatcgg tggtgtatgc caccgacacc gaaggctatg
     4801 tggggatgga tcgcaagctg gccgcttttg cgcgtgatgc agatgtgttg atccacgatg
     4861 cgcaatacag cgaggaacat taccgcggaa tgttggcggg attcccatcc acgcagggat
     4921 acggacattc gacagcgttg atggccgcgg aagtggccgc ttctgccgag acgggtgaac
     4981 tgatcctgtt tcatcatgac cccgcctact ctgacgattg ggtcgccgca caggaagccg
     5041 ccgcgaaaaa gttattccct gattcgtgcg cagcgtatga aggccttgag attcgtttga
     5101 aacccgaaca ggctggaatg gttttgactg gcccaacaca agcgagacgg ggtaatgttt
     5161 cccctcgctg aatggatgta aaatatgtta agaatgaccg aaatcaaacg cgacgatacc
     5221 cgcaacatcc tttcagacat taagttgttc gaagggctta gttctgcgca attggattgg
     5281 gtggcaaaac atgcgcaccg ccgcattttc actgcgggga ccaatgtgtt gacgatcgag
     5341 cagcccggcg aagcggtgtt cattattttg catggcacgg tgaagatcca cattgagcag
     5401 ggggagcggg atgtgattat ttcagtgctc ggaagcggcg acattctcgg tgaaatgagt
     5461 ttgatagaca gtgtcggccg ttcggcgagc gcgatcacgc ttgaaaattc gctcatgctg
     5521 tggatggaca agaccacctt ctaccatatt ctcgataact tcccgcctgt ggcgcgcaat
     5581 ttggtgaaaa tcttaaccgc acgtgtacgg ctttcagatc aggtcattca atcgctggca
     5641 acgctggatg tgaacgggcg ggtggcacgt caactgctgg cttttgccga gcggtatgga
     5701 cacgaaattg ccgacggtgt gcaggttcac atcgtgctga cacaaggcga cctggctgac
     5761 ctggtggggg catcgcgcaa acgggtcaat caggcgatgg tattcttcaa agagcaggga
     5821 ctcgtcagca cggatgcaga tgggcggttt gtgattcaca acaaggccgg gctcatgcat
     5881 ttttgcaatt gagtcggtct ggtagatgcg ctgttgccgt cagttgaaaa ccaattcaac
     5941 ggacggcaat ttaataacta ctcagctacc taactaacaa ctataatccc gccatgagcg
     6001 attacaaatt cttccatcct accgaagttc gctacggaga tcttgatccg cagggacatg
     6061 tgaacaacgc aaaatacctg acttatttcg agcaggcgcg catttactat ctgatcaaac
     6121 ttgggttatt caacaaggat cagtctttta tggaagtggg tgtcatcatc gccgatatcc
     6181 acatcaccta tcattccacc acccattacg gcgacgatat taaagtgggc gtgaaaatca
     6241 ccaggatcgg aaccaagtcc atcacggtgg agcaatgcgt gatggatgcg aagaccggca
     6301 aactgatggc cagcggcacc gtggtaatgg tcacattcga ttacgagggg ttaaagacca
     6361 ttcccgtacc ggaagaatgg aaacaaaaga tttcagcgtt cga
//
LOCUS       DAOQLC010000184         6390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5136_length_6390_cov_13.940489, whole genome shotgun sequence.
ACCESSION   DAOQLC010000184 DAOQLC010000000
VERSION     DAOQLC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5136_length_6390_cov_13.940489"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PL000_19680"
     CDS             complement(<1..448)
                     /locus_tag="PL000_19680"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91160.1"
                     /translation="MERQWQQGDWQNAGQGFDAVEGKLRLTSWSGARRVVVLRRRIKD
                     SMAAAETSNENLQPMLHFIDHSDKVKLWEYAILVTNTNYSLDAIGQLYRDRADCENGF
                     DELKNQWGWGGYTTHDLERCNLSARAVALIYNWWSWYVRLAHPKTRL"
     gene            540..1459
                     /locus_tag="PL000_19685"
                     /pseudo
     CDS             540..1459
                     /locus_tag="PL000_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    818..934
                     /locus_tag="PL000_19685"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(1512..4640)
                     /locus_tag="PL000_19690"
     CDS             complement(1512..4640)
                     /locus_tag="PL000_19690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNC91161.1"
                     /translation="MDFSKFFIDRPIFAIVLSIVIFAGGLIAIPLLPAGEYPEVVPPS
                     VVVRAIYPGANPKEMADSIAVPLEEAINAVEGIMYMKSVAGSDGSLQVTVTFTPGIDP
                     DTATVRVQNRVAQALSRLPPDVRLYGVTTQKQSPTPLMFVSLTSPDNRYDGVYLRNYL
                     TLNIKDQLSRLQGVGNVGLYGEGDYAMRLWLNPGKLATRGLTASDVINAVREQNVQVS
                     AGQLGAQPSPKSTDFLVPINVRGRLRSEQEFSDIVLKSGDHGQLVRLSDVGRVELGSG
                     DYTLHAMIDGKNGASAGIFLTPNANSLAVARAVYAKFEELSANFPAGVTYRIVWDPTV
                     FVRESIRAVGQTLIEAVFLVVLVVILFLQTWRASIIPLIAVPVSIVGTFAWLYLLGYS
                     INTLTLFGLVLAVGIVVDDAIVVVENVERYIEEGLDPLAAAHQAMKEVSGPIIAIALV
                     LCAVFVPMAFLSGVTGEFYKQFAITIAISTIISTINSLTLSPALAAKLLKRHDAPKDA
                     LTRLLERMLGWLFHPFNRLFLRSSHKYHNTVARTLGRRGIAFTVYAGLLLSTAILFHI
                     VPTGFIPNQDKLYLFAGAKLPEGASLARTNAVTHQLNKIASTIEGVDITESYVGLNAL
                     QSVNTPNQVTSYIILKPFDQRQRSAESITAELNAKLVEVKDGQAYALLPPPIQGLGNG
                     SGYSLYLVDRAGLGYGALQEALTTFQNAIAQTPGMTFPVSSYQANIPQLEVNIDRVKV
                     LAQGVLLSDLFNTLQIYLGSIYINDFNLFGRVYRVLAQADSVFRQKTEDISNLYTRNV
                     QGEMVPLGSMVTIQPSFGPDPVIRYNGYPAADLIGDADPRVLSSGQVITKLSSVAAEV
                     LPRGITLEWTDLSYQQVTQGNAAVIVFPLSIMLVFLVLAALYESWTLPLAVILIVPVC
                     MCAALFGIWLTGGENNIFVQVGLVVLMGLACKNAILIVEFARELEIQGKSTVEAALEA
                     CRLRLRPIIMTSIAFIAGSVPLVLSYGAGSEVRYATGITVFSGMIGVTLFGLFLTPVF
                     YVALRKLARLKP"
     gene            complement(4665..5834)
                     /locus_tag="PL000_19695"
     CDS             complement(4665..5834)
                     /locus_tag="PL000_19695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC91162.1"
                     /translation="MYSSTPRQRCAFTILIALSAIFLIGCNTGSNTPVESPPLDVAVA
                     NVLVRSVQTWDEFNGRVHAIPTVELRPRVSGYINRVAYKEDSEVKPGDLLFVIDPRPY
                     RNALHSAQAQLERARSTANLAQIKTEHAQALFETRAMSREDFHGRQTELNQTAAEIRA
                     AEAAVATAKLNLDFTEVRAPIAGRVGRAYLTVGNLAQADQSILTTLVSQDPMYVYFDC
                     DEHSFLRYKEFARRNDRGNNAYLVRIRLANEEGFPHHGTVDFFDNQVNPTTGTIRARA
                     VVSNTDRFLTPGLFARVQLQGDELLALLIDTKAILTDQHHKYIYVLGEGNKALRKNIT
                     LGRQIDDLYIVQSGLDKTDMVIVTGAQKIIREGMIVRPEYVAMEAREAISPLPVQ"
BASE COUNT         1602 a         1555 c         1753 g         1480 t
ORIGIN      
        1 ccaggcgtgt cttcgggtgt gccagcctga cataccaact ccaccaattg tagatgagcg
       61 cgactgctcg cgctgagagg ttgcaacgtt ccaggtcatg cgtggtatag ccaccccaac
      121 cccattggtt tttcagttca tcgaagccat tctcgcagtc ggcccgatcc cggtataact
      181 ggccaatggc gtcgagggag tagtttgtat tggtcaccaa tatagcgtat tcccagagtt
      241 taaccttgtc tgaatggtca atgaagtgga gcatcggctg caggttctca ttgctcgtct
      301 cggcagccgc catgctgtct ttgattcgcc gccgcagtac cactacacgc cgcgcaccac
      361 tccagctcgt cagccggagc ttgccttcca ccgcgtcaaa gccttgccct gcgttctgcc
      421 agtcaccttg ctgccattgc cgctcgatca gacgcttaac tccggcagtt tgtcgtagct
      481 tgaagaggtt gaatcgcccc gatatttccg gagataaaat gatttgagag gaggaagaga
      541 tgaagaatca aatcagttat tcaccggaag tacgagaacg agcagtaaga ttggtatttg
      601 agcagcaaaa ggagcatgaa tcgcaatggt cggcgatcaa atcgatcgca tcaaagattg
      661 ggtgtacacc ggagacattg cggacttggg taaggcggac ggagactgat caggggattc
      721 gtgcaggcat gtcgactgcg gatcgtgaga gactcaagga attggaacag gagaatcggg
      781 aattaaagcg agccaacgag atattacgta aggcatcggc ctattttgcg caggcggagc
      841 tcgaccgccg accgaaataa tggtgacatt tgtcgatagc aacaaagcgg aatacggggt
      901 cgagccaatc tgcaatgaaa tctggattgc cccgtcgagc tactacgaac acaaagcgcg
      961 cgcgcgagat cccgatcgat tgcctgaccg catcaagcgg gatatgagac tagaacttga
     1021 tatacagcgg gtatggaaag acaatttccg agtttatggt gctcgcaaag tatggcggca
     1081 gttgctgcga gaaggtattg gtgttgctcg ttgtacggtc gagcggttaa tgaagaagct
     1141 tggaatacaa ggtgttcgac gcggcaaaaa gtgttggaca accatttcgg acgatttgct
     1201 tgaccggcca acggacaagg ttaaccggca atttgtagct gcccggccca atcaattgtg
     1261 ggtggcggat attacatttg tagcgacatg gacaggattt gtttatgtgg cttttatcac
     1321 cgatgttttt gcccggtata ttgttggctg gcgagtgagt cgttcattgc gaacggaact
     1381 gatactggat gcattggaac aggcgctatg ggcacggcgg ggaacctcag gattgattca
     1441 tcacagtgat agaaagtcgg tttcacatga tgtgcggaag ttttggatat tgatctgagg
     1501 ctaattcgcc cctaaggttt gagacgagcc agcttgcgca aagcgacata gaagacaggg
     1561 gtaaggaaca atccgaatag cgtcacgccg atcatcccgg aaaaaacggt tatacctgtt
     1621 gcgtagcgca cctcactgcc tgcaccgtag ctgagcacta agggaacaga tcccgcgatg
     1681 aaagcgattg aggtcattat gatcggtcga agtcgaagac ggcaggcttc cagtgcggct
     1741 tcgacagtac tcttgccctg gatctccaac tcccgtgcga actcaacgat caggatggca
     1801 ttcttgcatg ccaggcccat cagcacgacc agtcccacct gaacgaatat attgttctcg
     1861 ccgccagtga gccaaatacc gaaaagcgca gcgcacatac ataccggcac gataaggatg
     1921 accgccaaag gtaatgtcca gctttcgtag agggcggcta gcaccagaaa gaccagcata
     1981 atcgataatg ggaatacgat gaccgccgcg ttgccctgcg ttacctgttg gtagctaaga
     2041 tcggtccact ccagcgtaat gccgcggggc aacacctctg cggcaacgct cgagagcttg
     2101 gtgatgacct gccccgagga aagtacgcgc ggatcagcat cgccgataag gtcagcggcg
     2161 ggatagccgt tgtagcggat gactggatcc gggccaaatg aaggttgaat cgtcaccatc
     2221 gaccctagcg gaaccatttc cccttgaacg ttgcgcgtgt acagattgct gatgtcttcg
     2281 gtcttctggc gaaaaactga gtccgcctga gccaacactc gatagacccg accgaataag
     2341 ttgaaatcgt taatatagat agaacctaga taaatctgca gggtattaaa tagatctgac
     2401 aataatactc cttgggctaa tactttaact cgatctatat tgacctcgag ttgcgggata
     2461 ttggcttgat atgagctgac aggaaaggtc atgcctggcg tttgggcgat tgcgttttgg
     2521 aaagtcgtta atgcctcttg taaagctcca tatcccaagc cggcgcgatc aactaaataa
     2581 agagaatagc ccgacccatt acccagtcct tgaataggcg ggggcagtaa tgcataagcc
     2641 tgcccgtcct tgacctccac aagcttggca ttcagttcgg cggtgatgga ttccgcagaa
     2701 cgctggcgct gatcgaaagg tttgaggatg atataggagg tgacctgatt gggagtattg
     2761 acggattgca aggcattcaa gcccacatag ctttcggtta tgtctacgcc ttcgatagtg
     2821 ctggcaattt tgttcagttg atgtgtgaca gcattagttc gggcaagcga agcgccctcc
     2881 ggcaatttgg ctcccgcgaa aaggtagagt ttatcctggt tgggaataaa gccggtcggt
     2941 acgatatgga acagtattgc ggtcgagagc aatagccctg catagacggt gaacgctatg
     3001 ccacgacggc ccagcgtgcg agccacggta ttatgatatt tatgggagct gcgaagaaat
     3061 agccggttga acggatggaa cagccacccc aacatacgct cgagcaaccg tgtcagcgca
     3121 tccttcggag catcatgtct tttgagcagt tttgccgcca gggccggcga caatgtcagt
     3181 gaattgattg tggaaatgat ggtggatatg gcgatggtga tagcaaattg cttgtagaat
     3241 tcgccggtga caccgcttag gaacgccatg ggcacaaaca cggcgcaaag caccagggct
     3301 atggcaataa tggggccgga gacttccttc atggcctggt gggctgctgc aagaggatcc
     3361 aggccttcct cgatgtagcg ctcgacgttc tcgacgacga cgatggcatc gtcgacgaca
     3421 atgccgactg ccaacaccag tccaaacaat gttagcgtat tgatggaata tccgagaagg
     3481 tacagccaag cgaaggtacc gacaatcgag accggtaccg cgatcagagg aatgatggat
     3541 gcacgccagg tctgcagaaa caggatgacc accagcacga ccaggaaaac cgcttcgatc
     3601 agggtttgac ccaccgcccg gatggattcc cggacaaata cggtagggtc ccacactatt
     3661 cgatacgtca cgcccgcagg gaagtttgct gaaagttctt caaacttggc gtagaccgct
     3721 ctggctactg ccagggagtt tgcgttggga gtgaggaaaa tgcccgcgct ggcgccgttc
     3781 tttccatcaa tcatggcgtg cagcgtataa tccccggatc cgagctcaac gcgtcccacg
     3841 tcggataagc gaaccagttg gccatggtcg ccgcttttca gcacgatatc gctgaattcc
     3901 tgttcgctgc gaagccgccc gcgcacattt atcggaacga ggaaatccgt actcttgggt
     3961 gacggttgcg cgcccagctg acccgcggat acctggacgt tctgctctcg aacggcattg
     4021 atcacatcac tggctgtaag cccgcgtgtg gcaagcttgc ccgggttcag ccacaagcgc
     4081 attgcatagt cgccttctcc gtacaatccg acgttgccaa caccctgcag gcgcgagagc
     4141 tgatccttga tattcagggt caggtagttg cgtagataga ccccatcgta gcgattgtct
     4201 ggtgaagtca gactcacaaa catgagcgga gtgggggatt gcttctgcgt ggttacgccg
     4261 tacagacgca catcgggggg cagacgcgac agggcctggg cgacgcgatt ctgtacacgg
     4321 acagttgcgg tatcgggatc gatgccaggg gtgaaggtga ctgtcacttg caggcttccg
     4381 tcggaacccg caacggactt catgtacatg atgccttcga cggcgttgat cgcttcttcc
     4441 aacggaacgg cgattgaatc cgccatttct ttgggattgg cgccggggta tattgctcgc
     4501 acaacgacac taggcggcac cacctctggg tactcgcccg cgggtaggag cggaatggcg
     4561 ataagtcccc ccgcgaaaat aacgatcgaa agaacaattg caaatatggg gcggtcgatg
     4621 aagaacttgg aaaaatccat gatcgcgctc ttattccgcg gctcttattg aaccggaagg
     4681 ggggagatag cctctcgtgc ttccatagcg acatactcag gccttacaat cattccttca
     4741 cgaatgatct tctgtgcgcc ggtgacaatg accatgtctg tcttgtcgag gccggactgc
     4801 acgatgtata ggtcatcgat ttgacgaccc aatgtgatat ttttgcgtag tgccttgttt
     4861 ccttctccta gcacataaat atatttgtga tgctggtcgg tcagaatggc cttggtgtct
     4921 atcaaaagtg caagtaactc atcgccttgc agctgtacac gggcgaaaag ccccggtgtc
     4981 aggaaacggt cggtattgga gacgacagcg cgagcacgaa ttgtgcccgt tgtgggatta
     5041 acctgattgt cgaagaaatc tacagtccca tggtgcggga agccttcctc attagccaga
     5101 cggatgcgga ccagatatgc attgttcccg cgatcgttcc tgcgagcgaa ttccttgtag
     5161 cgcaggaagc tatgctcatc gcaatcgaag tatacataca tgggatcctg cgagaccaag
     5221 gttgtcagaa ttgattggtc agcctgggca agattgccga cggttaggta tgctcgcccc
     5281 acgcggcctg cgattggcgc tcgaacctcg gtgaaatcga ggttgagctt ggcggtggct
     5341 acagcagctt cagccgcacg aatctcggca gccgtttggt tcaactccgt ttgtctaccg
     5401 tggaaatctt cgcgtgacat ggcccgtgtt tcgaacagcg cctgcgcgtg ctcagtcttg
     5461 atttgcgcaa gattcgcggt cgaccgggca cgttcgagct gcgcctgcgc actatggagt
     5521 gcattgcggt acggccgagg atcaatgacg aagagaaggt cgccaggttt gacctcgcta
     5581 tcctctttat aggcaacacg attgatgtaa ccactcacgc gaggacgcaa ttctacagtc
     5641 gggattgcat ggacacggcc attaaattca tcccaagttt gcactgaacg caccagtacg
     5701 ttcgcaaccg caacatcgag cggtggtgat tcgactggag tgttggatcc ggtattgcat
     5761 ccgatgagga agattgccga gagcgcaata agaattgtaa atgcgcaccg ttggcgtggg
     5821 gttgagctgt acatatgtta aatcatgaaa aatttctggc ggggagggtc gtaagactca
     5881 atcattctaa atgaaacctc tgcataccta tgtcattaag gttttttgct catgcggtaa
     5941 tttttaaaat caatcgttta aaaatgacaa attatccaaa aaaccagtta ggcagaggtc
     6001 tcaaatattc ctagtagagg atttccgaga aagaatgcat cggagaaaga ctaaattatc
     6061 ccgacttgaa cttgatctaa cagatggctt caagccgtaa tggttgcagg caagaaatct
     6121 ggcgctacct gatctaggga tggtctgaag tagacaccac catcttctag cggctttgcc
     6181 atcttgtcaa tgaaaaaaat tatttgctcc aggtcaactg tgtaagtatt gatatctgaa
     6241 ttccctttaa taaattacaa cgagatagaa attctatagc agaactatat ataaagctag
     6301 acggattggg tataggaaaa ataaaccacc ttttctaaac caccttttcc ataagttttg
     6361 aatgtactga aagctggctc aattgtcaaa
//
LOCUS       DAOQLC010000185         6371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5163_length_6371_cov_27.653420, whole genome shotgun sequence.
ACCESSION   DAOQLC010000185 DAOQLC010000000
VERSION     DAOQLC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5163_length_6371_cov_27.653420"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="chvE"
                     /locus_tag="PL000_19700"
     CDS             complement(<1..800)
                     /gene="chvE"
                     /locus_tag="PL000_19700"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNC91163.1"
                     /translation="MSKKLFYNLLAMLMLASLILSACGGGSSAPASDAKLVGISMPTK
                     TSTRWISDGESMVKSFEELGYRTDLQFADNDIPNQLAQIENMITKGANVLVIAAIDGS
                     TLSDVLAKAKEQGVLVIAYDRLISKTENVDYYATFDNFGVGVIQGTQIIEGLDLANQA
                     GPFNIELFGGSPDDTNAFYFYDGAMSVLQPYIDEGKLVVQSGQMGMDVVGTLRWDGTV
                     AQARMDNLLSANYTDKRVDAVLSPYDGLSRGIIAALRGVGYGTADQPWP"
     gene            complement(1039..2082)
                     /locus_tag="PL000_19705"
     CDS             complement(1039..2082)
                     /locus_tag="PL000_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC91164.1"
                     /translation="MVIADRRKRVTIKDVAQAAGVSTQTVSRVMNKFSYVSEETRQRV
                     ETVVEQLGYHPSTLARSLIQQRSYTLGVVTFGLKYIGPSRTLNGVADKADELGYMLLM
                     KELDNFDTNRIDEVIDSLLARQVDGIIWVAPEIGDNHAWVEERMHNIPAPVLFLAMKP
                     RAGISSVATDNYQGAVLAIQHLLECGRKKIGHISGPLTWWEADERKRGWFETLKAAGL
                     NPLEEHCAEGNWSSASGEQAFTQLLASYPDMDAVFVANDQMALSVLRETCCRGICVPD
                     QLAVIGFDNIAESAYFYPSLTTISQDLQLLGEQAVQSLVEMIQARQNNQVIVAQSRFI
                     QPTLVVRESSKRV"
     gene            complement(2324..3622)
                     /gene="eno"
                     /locus_tag="PL000_19710"
     CDS             complement(2324..3622)
                     /gene="eno"
                     /locus_tag="PL000_19710"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC91165.1"
                     /translation="MDTTIESISALEILDSRGNPTVEVEVVLMDGAMGRAAVPSGAST
                     GEHEALEMRDGDKKRYLGKGVSKAVANVNGVISEALFGWDASDQKAIDNELLALDGTP
                     NKAKLGANAILGVSLAVAKAAANSYGMPLYRYLGGVYAHVLPVPMMNIMNGGAHTSWQ
                     STDMQEFMVMPFGAPSFSEGLRWGAEIYHALKSVLKEKGYGALVGDEGGYAPALKANN
                     EAIELILAAIEKAGFKAGRGEQVAIALDPAASELYDEKTKKYNLRKEGKELTGEQMVE
                     FWAKWVKDYPIVSIEDGLAQDDWDAWKLMIKTVGDKCQIVGDDLLVTNPERVRRAIAE
                     NAANALLVKVNQIGSLTETIEAVDLCQRAGWRAVTSHRSGETEDATIADLAVALNTGQ
                     IKTGAPARSDRVAKYNQLLRIEAELGSEAHYAGWNALSNK"
     gene            3694..4374
                     /locus_tag="PL000_19715"
     CDS             3694..4374
                     /locus_tag="PL000_19715"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC91166.1"
                     /translation="MIAQQDFDPRDRKKYYLEETFARRFLLSLVRGVFHSVMVMKVEG
                     RENFPLDGPVIIAANHVTNFDVFPMQFSVSRAICYMGKAELFNTPLDSILRILCAFPV
                     YRGEKDEWAMRHAEKVLANGHTLGMFPEGKRSKGKGLGVAKTGTARLAIKMNCPIVPM
                     NVIGTDTFFKRFPYRSNVTVRLLPPILPNPNDTPLSLTDRLMFTIAEGLPESMRGVYA
                     ELPKGFGA"
     gene            4419..4907
                     /locus_tag="PL000_19720"
     CDS             4419..4907
                     /locus_tag="PL000_19720"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC91167.1"
                     /translation="MTQESSGNSTPAPKKLIPFLCYAKENQVIVREFSQRLKSEGWID
                     PWFDEEDILPGQVWQDSVVHAVRQSHAVIVFLSKEAVASEGFFQKELKLALDTAAEKP
                     EGTIFIIPIRIDHCEVPEMLAKYQYVDYFGDDEQKIRVYNSLIASLRNRAENLNILLN
                     PE"
     gene            5058..5213
                     /locus_tag="PL000_19725"
     CDS             5058..5213
                     /locus_tag="PL000_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNC91168.1"
                     /translation="MLFSPKEKGQGLVEYALILVLVAIVVIAALMILGPIIGNVFSKV
                     NSSLSNV"
     gene            5386..>6371
                     /locus_tag="PL000_19730"
     CDS             5386..>6371
                     /locus_tag="PL000_19730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC91169.1"
                     /translation="MTRKLRVFLCHASQDKPAVRGLYKRLAAESWIDPWLDEEKLLPG
                     QSFDHEIYNAARDSDIIIICLSKVSVAKEGYVNREIRRALDAADEKPEGAIFIIPLRL
                     DDCTPSFERLKQLHYADYFTPNAHEKLIKSLRLRADALKIETADSPVAVTPVTQTAAV
                     VTSPAAIKQTPGGHPIYTFGGMDFVKIPAADFFMGADDIEEAKPQHLVYQLNYDFYMG
                     RFPVTNLQYSKYTRELGQPVVLNKEKADHPVVNVTWHDAQNYIAWMNRKFRNELPAEF
                     AFRLPSEAEWERAARGTNGSEYPWGNSFDKNKCNSSESGIGDTTPVGKYSPQG"
BASE COUNT         1623 a         1571 c         1508 g         1669 t
ORIGIN      
        1 ggccagggct ggtcagctgt gccgtaacca acgccgcgca atgcagcgat gataccgcgg
       61 ctcagaccat cgtacgggga gagcactgca tcaacgcgct tgtcagtgta gttggcgctc
      121 agaagattgt ccatgcgggc ctgagcaacg gtgccgtccc agcggagggt accgacaaca
      181 tccatgccca tctggccgga ctgaacaacg agtttgcctt catcgatata gggctggagg
      241 acggacatgg cgccatcgta gaaatagaag gcgttggtgt cgtcggggga gccaccgaag
      301 agttcgatgt tgaaaggacc agcctggttc gcgaggtcga gaccttcgat gatctgggtg
      361 ccttgaatga cgcccacgcc gaagttgtcg aaggtggcat agtaatctac gttttcggtc
      421 ttggagatca ggcggtcata agcgatgacc aaaacacctt gttccttggc cttggcaaga
      481 acatcagaga gagttgaacc atcgatcgcg gcaatgacga gtacgttggc gcccttggtg
      541 atcatgtttt cgatctgggc aagctggttc gggatgtcgt tgtcagcaaa ttgcagatcg
      601 gttctgtaac cgagttcttc aaatgacttg accatgctct cgccgtcgga aatccagcgg
      661 gttgaggtct tggtcggcat ggaaatgcca acaagtttgg cgtctgacgc aggagcggaa
      721 gatccgccgc cgcatgcaga aagtatcagg ctagccaaca tcagcatggc tagaaggttg
      781 tagaacaatt tcttagacat ttcttctcct tagttagtaa gcttacgatg ttatattggc
      841 gtaataattg agcaagagca gatttgacca tcaagcagct gacgggtttg tctcgccaag
      901 gaagaatgca cttcatacgc caggcatcag agtgcatatc cttcgttttt atttacgagc
      961 cccttgttcc atcacctcct tttcaatgaa tgccgttcct gttttgaata tctgccaaag
     1021 ggacggaggg aaatacggtc aaacgcgttt ggaactttca cgtacaacca aagttggttg
     1081 aatgaacctc gattgcgcta cgatcacttg attattttgc cgagcctgta tcatttccac
     1141 taaactttgc actgcttgtt ccccaagtaa ttgcaagtcc tgggaaattg tcgtcaggga
     1201 tgggtaaaaa taagcggact cagcgatatt gtcgaagccg ataactgcca attgatctgg
     1261 gacacaaatg ccccgacaac aggtttctcg caaaacgctt aatgccattt ggtcgtttgc
     1321 aacaaagacc gcatccatat ctgggtatga tgcgagtaat tgtgtaaatg cttgttcacc
     1381 gctggcagaa gaccaatttc cttcggcaca atgctcttcc aaaggattta accctgccgc
     1441 cttcaaggtc tcgaaccaac ctcgtttgcg ttcatccgct tcccaccatg taagcggacc
     1501 tgagatatga ccgatcttct ttctgccgca ctccaataag tgttgaattg ccagaaccgc
     1561 accttgataa ttgtctgttg ccacactgga tattcctgca cgtggtttca tggcaagaaa
     1621 taacacgggg gcagggatat tatgcatccg ttcttcaacc caggcgtggt tatctccaat
     1681 ttcaggggct acccaaatga ttccgtctac ttgccgtgcc agtaatgagt ctattacttc
     1741 atctattcga tttgtatcaa aattatccag ttctttcatc aagagcatat agccaagttc
     1801 gtcggcttta tctgccactc cattaagcgt tcttgatgga ccaatatact ttagcccaaa
     1861 tgtgacaacc cccaacgtat aactgcgttg ttgtatcagg ctgcgggcca gtgtgcttgg
     1921 gtgatagcct aactgttcca caaccgtttc cactcgttgg cgagtttcct cagagacata
     1981 agagaacttg ttcatcacac gcgaaacagt ttgggtggaa acacccgcag cctgcgcgac
     2041 atctttgatg gtaactcgtt ttcttctgtc tgctattacc aaggactttt gccttcaagt
     2101 tatgttatcg ataacatgtt atcgataaca taataacaaa aatgttattt gaagtcaaga
     2161 gagagttttt tgaatttgta acactgaaat ttataaccca tttgggtgag gaactacgac
     2221 tagtattcag tggaataaac aactttagag tgtttgacga acttaaaata aaaagcacag
     2281 agagatttca tccgtgcttt ttattttatg aaatttaaat gttctacttg ttgcttaatg
     2341 cattccagcc agcataatga gcttcgcttc ccaattccgc ttcgatgcga aggagttgat
     2401 tgtattttgc aactctatca gaacgggcag gagcaccggt tttgatctgg cctgtattga
     2461 gtgcaacagc caggtcagcg atggttgcat cttccgtctc gccggagcgg tgagaagtga
     2521 ccgcgcgcca gcctgcacgt tgacaaagat ccacagcttc gatggtttca gtgagggagc
     2581 cgatctggtt gaccttgacg agcaaagcat tggctgcgtt ttcagcaatg gctctacgga
     2641 cacgttcagg gtttgtcacc aacaggtcat caccaacgat ctggcattta tcgccaacgg
     2701 tcttgatcat caatttccat gcgtcccagt catcttgcgc gagaccatct tcgatggaaa
     2761 cgatggggta atccttgacc cacttggccc aaaattcaac catctgttcg ccggtcaatt
     2821 ccttgccttc tttgcgaagg ttatactttt tggtcttttc atcatagagt tcagatgcag
     2881 cagggtcaag cgcaattgca acctgctcac cacggcctgc tttaaatcct gctttttcaa
     2941 tggcggcaag gatcaattcg atggcttcgt tattggcctt gagagcaggg gcgtatcctc
     3001 cttcgtcgcc aacaagtgcg ccatatccct tctctttcaa aactgatttc aaggcatgat
     3061 aaatttcagc gccccaacgc aggccttcgg agaaggaagg tgcaccgaag ggcatcacca
     3121 tgaactcctg catatcggtg ctttgccagc ttgtatgtgc gccgccgttc atgatgttca
     3181 tcattggaac agggagtaca tgtgcataca caccgccgag atagcgatac aaaggcatgc
     3241 cgtatgaatt tgctgcagct tttgcaacag caaggctgac gcccaaaatc gcatttgcgc
     3301 cgagtttggc tttgttgggt gtgccatcga gtgcaagtaa ttcattatca attgcttttt
     3361 ggtctgatgc atcccagccg aaaagggcct ctgaaatgac gccgtttaca ttggcaaccg
     3421 ccttcgaaac gcccttgccg aggtagcgtt tcttgtcgcc atcgcgcatt tccagtgctt
     3481 catgctcacc tgtggatgcg cctgaaggaa ctgctgcgcg ccccatggcg ccgtccatta
     3541 aaaccacttc cacttcaaca gtagggttgc cgcgtgagtc tagaatctca agagcggaga
     3601 tgctttcaat tgtagtgtcc attgctgact cctgaataag tatttgtgaa attacgtatc
     3661 aagtataatc gcattttgta tggagttgcc actttgattg cacaacaaga ttttgaccca
     3721 cgtgaccgaa agaaatatta tcttgaagaa acctttgcca gaagattttt gttgtcgctt
     3781 gtacgggggg tgttccattc cgttatggta atgaaggtgg aagggcggga gaattttcca
     3841 cttgatggtc ctgttatcat cgccgccaac catgtgacca atttcgatgt gtttccgatg
     3901 cagttttccg tttcccgggc gatctgttac atgggcaaag ccgaattatt taacacgccc
     3961 ctcgattcga ttttgcgcat tctatgtgct ttcccggtct atcgcggcga aaaagatgaa
     4021 tgggccatgc gtcacgcgga aaaagttctg gcaaatgggc acaccctcgg aatgtttcca
     4081 gaaggcaaac gttcaaaagg aaaaggtctc ggggtggcaa aaactggcac ggccaggttg
     4141 gcgattaaga tgaattgccc gatcgtgccg atgaatgtga tcggtaccga cacatttttc
     4201 aagcgatttc catatcgttc aaacgtgacc gtgagactgc tgccgcctat tttgccgaat
     4261 ccaaacgata cgccgctttc tcttaccgac cgcctgatgt tcaccattgc cgagggtttg
     4321 cccgaatcca tgcgcggagt gtatgcagaa ctgccaaagg gatttggtgc atgactggga
     4381 tataatctcg aaaaaaagca cgaaacggag ccaccatcat gacgcaagaa agttcgggga
     4441 attccactcc cgcccctaaa aagcttatcc cgtttctctg ttacgcaaaa gaaaaccagg
     4501 tgatcgtacg tgaattcagc caacgcttga aatctgaagg ttggatcgat ccatggttcg
     4561 atgaagagga tatcctgccc gggcaggtct ggcaggatag tgttgtccat gctgtcagac
     4621 aatcacatgc ggtgattgtt tttctttcta aagaagctgt tgcctccgaa ggattctttc
     4681 aaaaagaatt gaaacttgca ctggatacgg ctgccgaaaa accagaaggg acgattttta
     4741 tcatccccat tcgcattgac cattgcgagg tccctgagat gctggcgaaa taccaatacg
     4801 tggattattt cggcgacgat gaacaaaaga tacgggtcta caatagtttg atcgcatccc
     4861 tgcggaatcg cgcagaaaat ctcaacattc ttttgaaccc cgaataatcc gctattttac
     4921 aataccgtat tttttagtta acataactgt aaaggaaaac aacccaagcc cccttgaatt
     4981 atttttcaga ccgagtaaaa taagttcaat cctaggagac ctacctggga aattctgaag
     5041 aaaggagaca ctcaaccatg ttattttcac ccaaggaaaa aggtcagggt cttgttgaat
     5101 acgctctaat tctcgttttg gtcgccatcg tcgtgatcgc cgctctcatg atcctcggcc
     5161 cgatcatcgg caacgttttc agcaaagtaa actccagcct cagcaacgtg taattcacgt
     5221 ctgtctgtca aaaaatcccc gtcgcaaggc ggggattttt gttttttgct gggggaaaga
     5281 agcgttttag agacaattcc aaaagtggat tgctcaaaac gcataacccg ccgtgaaccg
     5341 atttataata cgcactatca tccactccat tgttggggaa gccccatgac ccgaaaactc
     5401 cgcgtatttc tctgccatgc ctcgcaagac aaacccgccg tgcgcggact atacaaacgc
     5461 ctcgccgccg aatcgtggat cgacccctgg ctcgacgaag agaaattgct tcccggtcag
     5521 agctttgacc atgaaatata caatgccgcg cgcgattcag acattatcat tatctgcctt
     5581 tcaaaggttt ctgttgccaa agaaggatat gtcaaccgcg agattcgccg cgcactcgat
     5641 gccgccgatg aaaaacccga aggcgcgatc ttcatcattc ccttgcgcct cgacgattgc
     5701 actccctcct ttgaacgcct caaacaactg cattacgccg attacttcac gcccaatgcc
     5761 catgaaaagc tgatcaaatc cctgcgcctg cgtgccgatg cgttgaagat cgaaactgcg
     5821 gatagccccg ttgccgttac acccgttact caaactgccg ctgttgtgac cagccctgct
     5881 gccatcaaac agacaccagg tggacatccc atttacacct ttggcggcat ggattttgtc
     5941 aaaattcccg ccgcggattt cttcatgggt gcggatgata ttgaagaagc caagcctcaa
     6001 cacctggttt atcaactcaa ttacgatttt tatatggggc gctttcctgt gaccaatttg
     6061 cagtattcca aatacacgcg tgaattgggt cagcctgtgg tgctaaacaa agaaaaggca
     6121 gatcacccag tagtaaatgt tacatggcat gatgcccaga attacattgc ctggatgaat
     6181 cgaaaattca ggaatgaact acccgccgag tttgcttttc gcctgccctc cgaagccgaa
     6241 tgggagcgcg ctgcgcgcgg aacgaacgga agtgaatatc cgtgggggaa ctcctttgat
     6301 aaaaataaat gcaattcaag cgagagcgga attggtgaca caacccccgt tggtaaatat
     6361 tccccgcagg g
//
LOCUS       DAOQLC010000186         6316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5244_length_6316_cov_11.263856, whole genome shotgun sequence.
ACCESSION   DAOQLC010000186 DAOQLC010000000
VERSION     DAOQLC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_6316_cov_11.263856"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PL000_19735"
     CDS             complement(<1..281)
                     /locus_tag="PL000_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91170.1"
                     /translation="MLQKKDGRYEVYGNPITERQSRAADAWKAMLAKKFNYDPNEKFN
                     LTVEDHPYGSEIFGLKNIVRDGSGQPVDKKNGVIVSTIRMGFGHYRIAMA"
     gene            complement(372..1592)
                     /locus_tag="PL000_19740"
     CDS             complement(372..1592)
                     /locus_tag="PL000_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC91171.1"
                     /translation="MKIQSYRFLQFLNKFLVYFVLTFFALVMIFPFLYMLATSFKIPA
                     DTFRYPPRMLPRNPVTMEVTGYDEPLPLYYVDVDGVQKQYALAKSNIKVGVYAPVADL
                     TATVERYLTEVKPTGGATNQQKVEVNGKQEKLFDVEVDGQVIPMILVSQTTVGEFIDP
                     QNPENKVFQNVRLSEPVENIGWHPENYVEVVELNNMARALTNTALVTILVVVGQLLTS
                     VVGGYAFARLKFPGRDTVFVFYLGTIMIPFVMLIVPLYQLMVLIGWTDRLAALVIPWI
                     FTAYGTFLMRQHFITFPKEIEEAALLDGASRWQILKEILIPASVPALATQATFTFLYA
                     WNSFFWPLVIINVGNERNHVLTLALNVLRGRASDTPNLILAGAAIAIVPPLIVFIFGQ
                     RFFVESVASSGVKG"
     gene            complement(1605..3278)
                     /locus_tag="PL000_19745"
     CDS             complement(1605..3278)
                     /locus_tag="PL000_19745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC91172.1"
                     /translation="MAAHSQVEPKTNLVLRIHFFWRIVFAVLAAAGLVSAWAGPFADA
                     DLWKKLIASAALLAASIFSVVGAVQISRRNHAGRMISLVLDYLAFVVCAVLVLNTGEV
                     FIGIDALGATFGKGIPWLGISVVGYFLSALDDYFPQKKQTSESSLKMVGRWVMLAGFA
                     IFLVQVGAINGILYFLGRLAQPVGVVSVAGALVFGISLWSMTRDDIALALHAKTSHEQ
                     MINGWLFLSPNLLGFLVFFAGPLILSFYFSFTDSDAFNTPNWVGFANYAKILNVRFAW
                     LSSPDQFAREVVDIKIYDEVGRFTFGDGGILFAAADKFFWLALRNTLTYVLFAVPLSV
                     IPALLLSNVLNSKLPGMKFYRAVYFMPSIAAVVGISLVWQWLYNATIGYINYFITVGI
                     EFWNNLFNAGVIDPKIQWVSDEKTALFAVILIAAWQTMGFNTVLFLAGLQNIPGELYE
                     AATVDGAGAWDKFWGITIPMLAPTTFYVVSTTTIQAMQVFEQVFILMNPAEGPNNSTI
                     TLVLYLYRSGFQNFQQGYASAIAWVLFIVIFGLTLIQFQRQRQSSIYES"
     gene            complement(3428..4825)
                     /locus_tag="PL000_19750"
     CDS             complement(3428..4825)
                     /locus_tag="PL000_19750"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNC91173.1"
                     /translation="MKNNLFLKALSLFVVASFILTACATPATEAPAATEAPAATEAPA
                     VTEAPAATEAPAADVALRWRTRPDNQEEIDVYSQISTELDAELDGITLTYEPGGSEGA
                     NYQDQLRAEIAAGTAPDVFWIPGTDVADFATRGLILNAADIAANSSDFSVDDFYAGPM
                     FHLTYNPESGASGADSGALWGLPRDVSTFALYLNMDLINEAGAPDPRELAANGEWDWD
                     AFLDVAQKTRALGSDVYGYGASAWWGPYGVWLNASGGGFFTEDRSACALNTEESLAGL
                     DFQRSLYQDYDVATPYGEDPEPPFRAGKVAMFQNGRWATPGVRTVDFEWDVVELPAGP
                     QGTAGNWLFWGAYVINANTEHPEEAWALVQALTTAQTQGKISALGANIPSRVSQEALD
                     AFLTFTPPANNQAFLNGIAPAANPTAEGPLWAGSWPEFDKIMGPAIQGVLTGATTVED
                     FGATICDEANKVFNQ"
     gene            complement(4898..5914)
                     /locus_tag="PL000_19755"
     CDS             complement(4898..5914)
                     /locus_tag="PL000_19755"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC91174.1"
                     /translation="MSTSHPTLRDVARLAGVSHQTVSRVINGSEEVLPETRAIVEEAI
                     KQLGYRPNAIARSMARGQTRTLACVSPNLTDYTFASVIEGAEVEARQHEYFMLSSSAT
                     DPQAFQELVNELVGHRRVDGLIVINPYADDRFLHLPENFPVVFVGARSHEKEICSVSL
                     DDEKVAYEATRHLISLGHTNIALVTGPMEEDCSQDRAEGYRRALQEAGIAYDESKVLE
                     GDWSASSGQRALLTFVENGPVPSAVFAQNDRMAMGVLRAARDVNLKVPSQLAVIGVDD
                     MPLSSYFDPPLTTMRQDMPLIGQEATRMLLDIINKKEPAVRELKLPAQLVVRQSTSDK
                     GGGS"
     gene            6010..>6316
                     /locus_tag="PL000_19760"
     CDS             6010..>6316
                     /locus_tag="PL000_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91175.1"
                     /translation="MVKITENQRKIQVSSPTYTWVWDAETDIFNLYDSQQRLISRARH
                     RPLIIGAEIFLDSQTSYQIKNDRIFIIYQATKNASRLTVSWSFQMDFISLEPLLLESP
                     "
BASE COUNT         1635 a         1666 c         1676 g         1339 t
ORIGIN      
        1 gccatggcaa tgcgataatg accaaagccc atacggatgg tgctgacgat cacgccgttc
       61 tttttatcca cgggctgtcc gctgccatca cggacgatat tcttcagccc gaaaatttcc
      121 gagccataag ggtggtcttc gactgtgagg ttgaactttt cgttggggtc atagttgaat
      181 ttctttgcca gcatggcctt ccatgcgtcc gctgcgcgag actggcgttc tgtaatgggg
      241 ttgccgtaca cctcgtatcg gccatccttc ttttgtaaca tgatgcgctt tctccttgaa
      301 attaaaataa ttgcataatt ttttgaaacc gccagccctg tttcggctgg cggtggttca
      361 ttatactttt tttatccttt gacaccgctg ctcgcaacac tttccacaaa gaagcgttga
      421 ccgaagataa aaacgatcag cggcggcaca atggcaatgg ccgccccagc cagaataaga
      481 tttggtgtgt cagaggcacg tccacgcagc acattgagcg caagggtgag cacgtgattt
      541 cgttcgttcc ccacgttgat gatgaccagt ggccagaaga acgaattcca cgcatataaa
      601 aatgtaaatg tggcttgtgt ggccagggct ggcacactgg cagggatgag gatctccttc
      661 aagatctgcc agcgggacgc gccatcaagc agtgcagcct cttcgatctc cttggggaag
      721 gtgatgaaat gctgacgcat gagaaaggta ccgtaggccg taaagatcca tggaatgacg
      781 agggcggcaa ggcggtctgt ccagccgatg agcaccatca actgatacaa agggacgatg
      841 agcatcacaa aggggatcat gatggtaccg agataaaaga caaagaccgt atcgcgcccg
      901 gggaatttca agcgggcaaa ggcataaccg ccaaccacag atgtaagcaa ttgccccacc
      961 accacaagga tggtcaccaa cgccgtgttg gtcagtgcgc gtgccatgtt gttcaactca
     1021 acgacctcaa catagttttc aggatgccag ccgatattct ccacaggctc gctcaggcgg
     1081 acattttgaa aaaccttgtt ctcaggattc tgagggtcaa tgaactcacc caccgtggtc
     1141 tggctgacca ggatcatcgg aatgacctga ccatccacct caacatcgaa cagtttttcc
     1201 tgctttccgt tcacttccac tttttgctgg ttcgtcgctc cgccggtcgg tttcacctct
     1261 gtcagatagc gttccacggt ggcagtgagg tcggctacgg gagcatacac accaaccttg
     1321 atgttgcttt tggcaagggc gtattgcttt tgcacgccgt ccacgtccac gtaatagagc
     1381 ggaagtggct catcatagcc agtcacttcc atggtcaccg ggttgcgggg cagcatacgc
     1441 ggtggatagc ggaaggtatc cgcagggatc ttgaaggatg tggcaagcat gtacaggaac
     1501 gggaagatca tcaccagtgc aaaaaaggtg aggacaaaat acacaaggaa cttgttcagg
     1561 aattgcagaa agcgatagga ttggattttc atggcggctc ctgtttagct ttcgtaaatg
     1621 ctcgattggc gctgacgctg gaactggatc aacgtgagcc cgaagatgac gatgaacaga
     1681 acccatgcga tcgcagaggc atatccctgc tggaagttct ggaaaccgct gcggtacaaa
     1741 tacaacacca atgtaatggt ggaattgttc ggcccttcgg ctggattcat caagataaag
     1801 acctgctcaa aaacctgcat ggcctgaatg gtggtggtgg aaaccacata aaaggtcgtc
     1861 ggcgcaagca tggggatggt gattccccag aacttatccc atgcgcctgc accgtccaca
     1921 gtggcagctt cgtacaattc accggggatg ttctgcaacc ccgcaaggaa aagcaccgtg
     1981 ttaaaaccca tcgtctgcca ggcagcgatc agaatcacgg cgaacagggc cgtcttttca
     2041 tctgataccc attggatctt cgggtcgatc acaccggcat tgaacaggtt gttccaaaac
     2101 tcaatgccca ctgtgatgaa gtaattgatg taaccgatgg tggcgttgta cagccattgc
     2161 cagacgagtg aaataccgac aaccgcggca atgctcggca tgaaataaac agcacggtaa
     2221 aacttcatcc cgggcagttt gctgttcagc acattcgata ataacaaggc ggggatcacg
     2281 ctcaaaggaa cagcgaacag gacataggtc agtgtgttgc gaagcgcgag ccagaagaat
     2341 ttatcggcag cggcaaacaa tattccaccg tcgccaaagg tgaagcggcc gacctcgtca
     2401 taaattttga tatccacaac ctcacgcgca aactggtcgg gagaggaaag ccacgcaaag
     2461 cggacattca ggatcttggc gtaatttgcg aagcccaccc aattgggcgt gttgaaggca
     2521 tctgaatcgg tgaatgaaaa atagaacgac aggatcaacg gccctgcaaa aaaaacgagg
     2581 aagcccagca ggttcggcga taaaaagagc cagccattga tcatttgctc gtgactggtc
     2641 tttgcatgta aagccagcgc aatatcatca cgggtcattg accatagcga gatgccaaaa
     2701 accagcgccc ccgccaccga taccaccccg acgggctggg caagcctgcc aaggaaataa
     2761 agaatgccgt tgatcgcccc aacctgcacg aggaagatgg cgaaccccgc cagcatcacc
     2821 caacggccca ccattttcaa gctggattcg gaggtctgtt ttttttgcgg gaagtaatca
     2881 tccagcgcgc tcaaaaaata gccgacaact gagatgccca accagggaat tcccttcccg
     2941 aaagtggcac ccagtgcatc aattccgatg aagacttcgc ccgtattcag cacgaggacg
     3001 gcgcaaacca caaaggcaag ataatccagc acgagtgaga tcatcctgcc cgcatggttg
     3061 cggcgtgaga tctgcaccgc tcccaccacg ctgaaaatgg aagcagccag caaagccgcc
     3121 gacgcgatga gtttcttcca aaggtcggca tcagcaaatg gccccgccca ggccgaaacc
     3181 agaccagccg ccgccaaaac ggcaaacacg atccgccaga aaaaatggat gcgtaacacg
     3241 agattcgttt taggttctac ttgtgagtga gcggccattt tcacatctcc acaggttcgg
     3301 ggctcaagga tgcagcgcac aaaaaaatca cgaaatcaca aaaaagtttt gtagcttggg
     3361 gatttcttta tgttttaaag agagaggggc aggcagtgct gcctgcccct ttttgttgca
     3421 aggaatatta ctggttgaac accttgttgg cttcgtcgca gatggtcgcg ccgaaatctt
     3481 caacggtggt tgcaccagtc aacacaccct ggatggccgg gcccatgatc ttgtcaaatt
     3541 caggccagga accagcccac agcggacctt cagcggtcgg gtttgcagcg ggggcaattc
     3601 cgttcaggaa ggcctggttg ttggcaggcg gggtgaaggt gaggaaggca tcaagtgctt
     3661 cctggctcac gcggctcgga atgttggcgc cgagagcaga gatcttgccc tgggtctgag
     3721 cggtggtgag agcctgtacg agagcccagg cttcttcagg atgttcggtg ttggcgttga
     3781 tcacatacgc gccccagaac aaccagttac cggctgttcc ctgaggacca gcgggcagtt
     3841 caacgacatc ccattcgaag tccacagtgc ggacacccgg ggtcgcccaa cggccattct
     3901 ggaacatggc aaccttgcca gcgcggaacg gaggttcagg atcttcaccg tacggggtgg
     3961 ctacatcgta gtcctgatag agggagcgtt ggaaatcgag accagcgagg gactcttcgg
     4021 tgttgagggc gcaggcggag cggtcttcag tgaagaaacc accgccggaa gcattcaacc
     4081 acacaccgta aggaccccac caggcgctcg cgccgtaacc atacacatcg gaaccaaggg
     4141 cacgggtctt ctgagccaca tcgaggaagg catcccagtc ccattcgccg ttggcggcga
     4201 gttcacgagg atcgggagcg ccagcttcgt tgatcaagtc catgttcagg tacaaagcga
     4261 aggtggatac atcgcgggga aggccccaca aagcgccaga gtcagcgccg gaagcgccag
     4321 attcggggtt gtaggtgagg tggaacatcg gaccagcgta aaaatcatca acgctaaagt
     4381 cggagctgtt ggcagcaatg tcagcggcat tcaagatcaa accacgggtc gcaaaatcgg
     4441 caacatcggt tccagggatc cagaaaacat cgggagcggt accggcggca atttcagcgc
     4501 ggagttggtc ttgatagttg gcaccttcgg aaccacccgg ttcgtaggtg agggtgatgc
     4561 catcgagttc cgcatccagt tcggtgctga tctggctata gacatcaatt tcttcctgat
     4621 tgtcagggcg ggtgcgccag cggagagcta catcggcggc gggagcctcg gttgcagcag
     4681 gggcttcggt cactgcgggt gcttcggtgg ccgcaggtgc ttcagttgca gcaggagctt
     4741 cggtggccgg ggtggcgcaa gctgtcaaaa taaaagatgc aactacgaaa aggctgagag
     4801 ccttcaaaaa cagattgttc ttcatttatt cttctccttg aaaatataga gcgatagact
     4861 tacaaaaagc ggtttccatc agtatcttcc tctgaaacta ggaaccacct cctttatcag
     4921 atgtagattg tcgtacaacc aactgagcgg gtaatttcaa ttcgcgaacc gcaggttcct
     4981 tcttattaat aatgtccagc aacatgcggg tggcttcctg tccgatcaag ggcatatcct
     5041 gacgcatcgt ggtcagcggt ggatcaaagt aagatgacaa gggcatatcg tccacaccaa
     5101 taacggctaa ctgagaaggg actttcaaat ttacatcgcg ggccgcacgc aacacaccca
     5161 ttgccatgcg gtcattttga gcaaacacgg cggaaggtac aggaccattt tcaacaaacg
     5221 tgagcaatgc ccgctgaccc gaagaagccg accaatcccc ttccagcacc ttggactcgt
     5281 cgtatgcgat gccagcttcc tgcaaagcgc ggcggtatcc ttctgcacgg tcttgcgaac
     5341 aatcctcttc catcggaccc gtaaccagtg caatgttggt atgacccagc gagatcaaat
     5401 gacgggtggc ttcatacgcc actttttcat cgtccaatga aacagagcaa atttcctttt
     5461 catgcgatcg cgcacccacg aaaaccacag ggaaattttc agggaggtgc aaaaatcggt
     5521 catcagcgta tgggtttatg acgatcaacc catctacacg gcgatgcccc accaactcat
     5581 tcaccaattc ctgaaacgcc tgcggatcag tagcagaaga agacagcatg aaatactcat
     5641 gctgtctggc ttccacttca gcgccttcga tgacgctggc aaaggtgtaa tctgtcaaat
     5701 tgggggaaac gcaagccagg gtgcgggtct gaccgcgggc catggaacgg gcaattgcat
     5761 tggggcggta tcccaactgt ttgatagcct cttccacaat ggcacgagtt tcgggcaaaa
     5821 cctcctcact cccgttgatc acacgcgaaa ccgtctggtg cgaaacacca gccagccgtg
     5881 caacatcacg aagagtgggg tgagaagtgg acataaatca ggtgggggtt gtataatgtt
     5941 accgttcacg tgaacggtaa cattagttta caagaacgac aatgctttgt caagaggcaa
     6001 tttctcaaaa tggtcaaaat caccgaaaac cagcgcaaaa tccaggtctc ctcccccacc
     6061 tacacttggg tatgggacgc cgagaccgac atattcaatc tgtacgactc gcagcagcgg
     6121 ctcatctcac gcgcgcgcca tcgtccgctc attatcgggg cggaaatatt tctcgactca
     6181 cagaccagtt atcagattaa gaatgaccgc atctttatca tctatcaagc gacaaaaaac
     6241 gcctcgcgct taactgtgag ttggtccttc caaatggact ttatctccct cgaaccgctt
     6301 ctgctcgaat cccccg
//
LOCUS       DAOQLC010000187         6314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5248_length_6314_cov_22.821697, whole genome shotgun sequence.
ACCESSION   DAOQLC010000187 DAOQLC010000000
VERSION     DAOQLC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_6314_cov_22.821697"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..519)
                     /locus_tag="PL000_19765"
     CDS             complement(316..519)
                     /locus_tag="PL000_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91176.1"
                     /translation="MREKDFLFAKKNLIAQLNKNKTDALDKTDALNKTDALNKTDALD
                     KKIVRAKYRSSIYSNSSNSSNSP"
     gene            complement(520..1143)
                     /locus_tag="PL000_19770"
     CDS             complement(520..1143)
                     /locus_tag="PL000_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91177.1"
                     /translation="MCNFNTCVQGIEINDGNYKFELNNIVYTTELGFGLITIGNTSFK
                     CELILVYNEKYNNDYGLTPMLHDIDNFIFDDLKSKDTVFYILNMNESISETCPGYLIK
                     NNIKYEFYLIIRYTDDVVNKLKELQIYPFIPHLKSVNHYLNLSNQHVSIMNLEMSNLL
                     ESNYLIESNLIKVNKSRGKQRNFGIFLLTTEEYKCGHILIIKNKTDD"
     gene            complement(1457..1528)
                     /locus_tag="PL000_19775"
     tRNA            complement(1457..1528)
                     /locus_tag="PL000_19775"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1493..1495),aa:Glu,seq:ttc)
     gene            complement(1845..2495)
                     /locus_tag="PL000_19780"
     CDS             complement(1845..2495)
                     /locus_tag="PL000_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91178.1"
                     /translation="MDFILILKKLAFIKIRKELENELDEIIDIKKIKGKLVGDIIEVK
                     EKIKKMVKEHSDYVINVAANRKEEEEISDEEISDEEISDEEISEEVKKKKKIKFHFFF
                     LRKARESDNTIIYQHHAGLYIVKVRVRKFIRKNSLRRRRFSNFKSIKNLLNFVRGKYK
                     KKLKKSNKEKKKSIYNFYKNSNLILRTKLLKEIDKNILNKSKYEFKKYFNIFKIFL"
     gene            complement(2730..3515)
                     /locus_tag="PL000_19785"
     CDS             complement(2730..3515)
                     /locus_tag="PL000_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91179.1"
                     /translation="MIVQAQDYPCDCSPLQQYYCFKNRAKPQSIIHCGCSRLDQYCCS
                     KSERFCSSYLYASSEPTYTIHNKRCVLIKNSSINIHIQGRFFSDTIKESEKKTIKEIR
                     FAKQACIMCVLEAIYDNAIELKSLYYEIKDLKRKRKRTEEENLELESKLNLIQEKHNL
                     ILKKKLNLFLILKSFKNDAQRATTYNFRGVILELIESAYDNTYNLYMVDDLCKYKIYV
                     DTSEGVIRKFKPKKPEDINITEDINTTEIIEESKEEKQNHDNR"
     gene            complement(5777..6190)
                     /locus_tag="PL000_19790"
     CDS             complement(5777..6190)
                     /locus_tag="PL000_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91180.1"
                     /translation="MKSGFILNNDYSNFFLHFFNLIKFSNIINKQINYINTFNLLYKY
                     NICLKQIKYIHIINHYNNFFFKYKKKFELPILFIKNIRQKRKLRSKKKYNKKIRGSYN
                     VEGFLNKYKKRNTLKKIINNMYLNKVIKLKKMPIK"
BASE COUNT         2207 a          616 c          429 g         3062 t
ORIGIN      
        1 ttactcacct atacgctatt tttttcaaat ttaacttgca tgtgttatgt atattaatag
       61 cattcactca aagctaggat caaactcttt caagataaaa aatattttat tatctttttt
      121 tttaattttt aactatatat ttattttctc cataagatat tcttctttat aaattatttc
      181 ccataaaaat gcatgggaga agaagataaa atataagata ttcttcttta ttgaaaaata
      241 tcttatattt tatcttcttc tttaaataaa ttatttaaat aatttattta attttttcta
      301 cttgaagaac agagcttacg gagagtttga agagtttgaa gagtttgagt agattgagga
      361 tctatattta gctctgacta tttttttatc taaagcatca gttttattta aagcatcagt
      421 tttatttaaa gcatcagttt tatctaaagc atcagtttta tttttattta attgtgctat
      481 taaatttttt tttgcaaata aaaaatcttt ttctcgcaat caatcatctg ttttattttt
      541 aattattaaa atatggccac atttatattc ttccgttgtt aaaagaaaaa ttccaaaatt
      601 tctttgttta cctctagatt tatttacttt aattaaatta gattctatta aataattaga
      661 ttctaataaa ttactcattt ctaaattcat tatacttaca tgttgatttg ataaatttaa
      721 ataatgatta accgatttta aatgtggaat aaatggatat atctgtaatt cttttaattt
      781 attaacaaca tcatcagtat atctaattat taaataaaat tcatatttaa tattattttt
      841 tattaaatat cccggacaag tttctgaaat gctttcattc atattcaaaa tataaaaaac
      901 tgtatcttta gattttaaat catcaaatat aaaattatca atatcgtgta acattggagt
      961 taaaccataa tcattattat atttttcatt ataaactaat attaattcac atttaaatga
     1021 ggtattacca attgtaatta aaccaaaacc taattcagtt gtatatacaa tattgtttaa
     1081 ttcaaactta taattaccat cattaatttc tataccctga acacatgtat taaaattaca
     1141 catattgtta tctaaacaat tgtaatttaa gtaaaaatcg tttaatatta aaaaaaaaac
     1201 gattacaaac atttttataa aatttttaat ttttatcatt attattattt attttatatt
     1261 ataattttat tactttactt taacctttac tttactttac caccttcact tggacctggc
     1321 atggggttgg ggggtgaagg gctagccccc acctctatac atgcacaaac cacaaaacgt
     1381 cttaaaaata attacttttt tttttctttt ttaacttatt tataaggttt tttggggttt
     1441 ttttgggttt taaattttag ctctacggga attgaacccg tgtttttaca ttgaaaaggt
     1501 aacgtcctaa ccactagacg aagagctaaa tagaaaaaaa aatattaaat tatatgttta
     1561 aatttatatt cgctattttg aattaattta tattcttttc tatttttttt tgttttttta
     1621 tttacatatt taaatttttt atgatgaaat attctaaaag aaaaacgtga aattacagta
     1681 tgagatttac gcataaatct gtattctaat tttgaagctt taagaatatt ttcaaaaaac
     1741 atttttaaat ttaaaattaa tttaaatatt atttttttta aaattttttt ttctaagaat
     1801 tttaaaaaaa ttttactttc tataaaattt ttttgttttt ttattcataa aaatatttta
     1861 aatatattaa aatatttttt aaattcatat ttacttttat ttaaaatatt tttatcaatt
     1921 tcttttaata atttagttct taatattaaa tttgaatttt tataaaaatt atatattgat
     1981 tttttttttt ctttattaga tttttttaat ttttttttat atttacctct tacaaaattt
     2041 aacaaatttt ttatagactt aaaattggaa aatcttcggc gtcttaaaga attttttcta
     2101 ataaattttc taactctaac tttaactata taaagacctg catgatgttg atatataata
     2161 gtattatcag attctcgtgc ttttcttaaa aaaaaaaaat gaaattttat tttttttttt
     2221 ttttttactt cctcgcttat ttcttcgtca cttatttctt catcacttat ttcttcgtca
     2281 cttatttctt cttcttcttt tcgatttgct gctacattta ttacataatc actatgttct
     2341 ttaaccattt ttttaatttt ttctttaact tcaataatat caccaactaa tttacctttt
     2401 atttttttta tatcaataat ttcatctaat tcattttcta attcttttct tatttttata
     2461 aatgctaatt tttttaatat tagaataaaa tccaataaat ttaaattttt aaattcttga
     2521 tttttatctt cttcttttac ttcatttaaa atttccctag caaatttaaa aaagttatta
     2581 aataactttg catcttctaa ttttaattcc gaaacattat ttgatgaaat attatactct
     2641 tgtacaagcc ttaaaacatc taatacacaa gaatctttgt tgaatttatt taatttttta
     2701 ttaaattcaa gacacttatt taatgctttt catctattat catgattttg cttttcttct
     2761 tttgattctt ctattatttc agttgtattt atatcttcag ttatatttat atcttcaggt
     2821 ttttttggct taaattttct tataacacct tctgaagtat caacatatat tttatattta
     2881 cataaatcat caaccatata taaattatat gtattatcat atgcagattc tattaactct
     2941 aaaattactc ctctaaaatt ataagtagtt gctctttgtg catcattctt aaatgatttt
     3001 aaaatcaaaa aaagatttaa tttttttttt aaaattaagt tatgcttttc ttgtattaaa
     3061 tttaattttg attctaattc taaattttcc tcttcagttc tttttcgttt tctttttaaa
     3121 tcttttattt cataatataa tgattttaat tcaatagcat tatcataaat agcttctaaa
     3181 acacacatta tgcaagcctg tttggcaaat cttatttctt taatagtttt tttttcagat
     3241 tcttttattg tatctgaaaa aaaacgacct tgtatatgaa tatttatgct tgaattttta
     3301 atcaaaacac aacgcttatt atgaatagta taagtaggtt ctgaagaagc atataagtaa
     3361 ctagaacaaa atctttctga tttagaacaa caatattgat ctaatctaga gcaaccacaa
     3421 tgtattatag attgcggttt tgctctattt ttgaaacaat aatattgttg taaaggagaa
     3481 caatcacaag ggtaatcttg cgcttgtact atcatagaat atcaaaattc caaatttaat
     3541 tcatttaata ttttattttc attattattt ttcatttgat attttaaaat tctaaaaaca
     3601 tgaactggaa tatatcttgc ataaatagtc atttatcaat aaataggttt taaatatcgc
     3661 attctaggta acttatttga aattacacga gttttatttg aaatataacg ctttcaaatt
     3721 cagcggcgta ttcgagacat ttttaaatct tttattctga attctaaagt gcgcatttct
     3781 aataaaggtg aataatttca ttcaactaaa ttaggagatg ttcataaaaa agattttatt
     3841 ttcatactat ttgttaattt taattcatat cgtataggtt caatattata atgacctaaa
     3901 tattttgaat atctataata tgatctatga tcatacttac aaaataaaaa ttctttggtt
     3961 aaattaaaaa ttattaaatt ttcttttttt caacgcctta atcattttaa atatttatct
     4021 ttttctttaa atttatttca attttgtact gtaaataaca tatttactaa agtattattt
     4081 ttaatataaa ttattttttc aatattttca atttttaaaa tttttttttt ttgaatattc
     4141 ataatatact ttatataaat ataaattgaa tattttttta cataataatt atttaattta
     4201 taaaaacgca tttaattata tattaaattt ataaaaatta aaatactaaa attttataaa
     4261 atgcttactc aattaatatt atttttagta tttttaacct gtaaatttaa tatataatta
     4321 tttaatttat aaactatttc ttgtttactt ttgtttttag tattcataaa aaaattaaaa
     4381 ttatcattat ttataatatt aaaaacaaaa gaatatttta ataaattact atttaaaaaa
     4441 tattttataa aattcaattt aaatataata tattcaaaaa aagtaaattg attatacata
     4501 ctaaaaaaat aatttttttt ttttttttga tgaataaata atcgtcttaa attttttcaa
     4561 tgtaatattt ttttattatt acgttttatt caatatttat tattaaatct acgttttatt
     4621 ttaaaattac tttgaggatg ctttattaat tttaatcttc taattttaga ataattgcta
     4681 tattgtcatc aaattgtgtt cattaattta taatttatat taattttttt taataaaaaa
     4741 aatttatctt taaataattt taatgaagta tttaatttat ttttatataa attattaata
     4801 ttattcttaa attttagtaa tattaataaa tatttttttt ttattacttt aatttttatt
     4861 aatttattat ataaatattt aaatcaattt tttaaattat aatctttttt tattaaagtt
     4921 aatattttta attttcattt attttttaat attatttttt tatttttttt tttttgctta
     4981 atattttttg ctacttcttt ttttaatatt ttaaaaatat tattttgtaa ttttttatat
     5041 ttatgcttat tttttaaaac cataattgtt tttataaatt ttaaaaattt aaaatttata
     5101 taatgtttat aaaatcataa acaaaaatta gtttttgaat aaaattttaa agattgcatt
     5161 aattttaata attgtataat acctaaaatt tttttaatta caaaaaattt tgataaagta
     5221 ttatatttaa ataaaaaaaa tttattaaca tttttttgtt tttttttttt taattttaaa
     5281 agctttttta tatatttttt tatttttcaa tgccttttac ttttataact atttaaagat
     5341 actcacaatt ttggtggaag tcattttaat tttggaacta gttttatttt attttttatt
     5401 ctaaaaatca ttttattttc tattttattt tctattttat tttctatttt attttctatt
     5461 ttatttttat tttttaattc attatgatta ttagtattaa tttgtttttt attttttcaa
     5521 ttttttttat ttttatgttt ttttctacga ttatgtttat ttcataaaat atattttcac
     5581 attaaacgat ttttaaaaat ttttattttt ttgtgttttt ttttatttgc tatttttttt
     5641 atttttaata aatattttaa attttttttt tttttttttt ataatataaa taattttcta
     5701 tatatttaga agttattttt tttaatattt tattattact aatttttttt acaaaattaa
     5761 cttttcttgc tttttttcat ttaattggca ttttttttaa ttttattact ttatttaaat
     5821 acatattatt tataattttt tttaaagtat ttcttttttt atatttattt aaaaaacctt
     5881 caacattata acttcctcta atttttttat tatatttttt tttacttctt aattttcttt
     5941 tttgtctaat attttttata aataatatag gtaattcaaa ttttttttta tatttaaaga
     6001 aaaaattatt ataatgatta attatatgaa tatatttaat ttgttttaaa catatattat
     6061 atttatacaa tagattaaaa gtatttatat aattaatttg tttatttata atgttagaaa
     6121 attttattaa attaaaaaaa tgtaaaaaaa aatttgaata atcattattt aatataaaac
     6181 ctgatttcat taaattaaat tttttttttt ttaaataatt atatttttca taataattta
     6241 tataaatttt tggaaatatt catttacctt ctatcatatt aaaatttgaa ataaaaaaaa
     6301 cacttaattt ttta
//
LOCUS       DAOQLC010000188         6299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5266_length_6299_cov_21.673126, whole genome shotgun sequence.
ACCESSION   DAOQLC010000188 DAOQLC010000000
VERSION     DAOQLC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5266_length_6299_cov_21.673126"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="PL000_19795"
     CDS             complement(<1..1121)
                     /locus_tag="PL000_19795"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNC91181.1"
                     /translation="MKYTDLKIQTQREAPNNARTEGFALLVRAGYMTRECEILPLGKE
                     ALTRLETLSNDPSFSEVVDLPRLVSEQAVFFPMVTGNLEVAHCPSCKYTELLDMAKFK
                     KTPLPHEEERPLEKVLTPDCNTIESLANFLGLPKEKTAKALMFTRKEDGKFVFAAVRG
                     DMQLSEAKLEYIVGEIQPANAEAISRSGAVAGYASPIGLNDALILVDDLIPQSQNLAA
                     GANEAGYHLLNTNYPRDYSAEIVEDLVMAKAGDTCSNCGNALEVVSAMPLGTRFGLYY
                     ENWLLALAERHHDDKGLTFPKSAAPFDVYLMHVPGKQMDTRAEAEKIYNQLQNAGISV
                     LFDDRDERAGVKFNDADLIGCPIRVTVGEKALQNGMVEL"
     gene            complement(1176..>1691)
                     /locus_tag="PL000_19800"
     CDS             complement(1176..>1691)
                     /locus_tag="PL000_19800"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNC91182.1"
                     /translation="ASEAVQPVQAVERITSVLGSGLIWHGSINGSGGVRIEGAFEGEI
                     ALRGMLVVGETGRVTCQNVRANTVIVAGAVRGNITTQKLEIRASGRVWGDVVTTAFVT
                     EEGAFLRGQIRMEETVDLDLEPAPESTPSEAAQAESIAQTPIQISTPEPTLPTDSTQK
                     LMRNGKKKSEV"
     assembly_gap    1692..1791
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1912..2709)
                     /locus_tag="PL000_19805"
     CDS             complement(1912..2709)
                     /locus_tag="PL000_19805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNC91183.1"
                     /translation="MGLLEGKNALIFGLANDKSIAWGITQAFKREGANIGISYAGEML
                     ERRVKPLAEQVDCQWLEECDITKDDHIAQTVEKAAKHFGKIDVLVHSIAYAGRDELSK
                     PFHETSREGFKTALEISAFSLVALTHAFLPIMNPGASVMCMTYYGSVKVAPHYNVMGV
                     AKAALESSTRYLAYDLGPQKVRVNAISAGPIRTLAAAGVGGFRDMYKHFADMAPMREN
                     VTIEDVGNSAVFLASDLSAHITGEVLYVDSGFNIIGVQVSEKDEKGG"
     gene            complement(2745..3323)
                     /locus_tag="PL000_19810"
     CDS             complement(2745..3323)
                     /locus_tag="PL000_19810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HNC91184.1"
                     /translation="MTLPRKFYNRPTLTVARELLGARLVRISDGMKLVGIISETEAYF
                     GFDDLASHAKAGRTIRTDPMFGPPGLAYVYFTYGNHWMLNAVTEKEGFPAAVLIRAIQ
                     PLEGVDVMMERRQGRDTLGPGKLTQALGITKSENYADLTESASSLRIEAGFKVPDKCV
                     TIGPRVGLNTTPEPWFSKPWRFLVKETIADIE"
     gene            complement(3386..4309)
                     /locus_tag="PL000_19815"
     CDS             complement(3386..4309)
                     /locus_tag="PL000_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC91185.1"
                     /translation="MNFLITGAAGFLGSSLANHLAREGHQVRGLDDLSTGDPKVLAPD
                     VHFTRGDVSDRPKLWTLLQDVDVVYHLAARVSVQESILYPRDYNAVNVGGTVALMEAM
                     RDVGVKRVVLASSGAVYGDLGDSTLTESTRPNPRSPYAVSKLAAEYYVNTIGSLWKIE
                     TVSLRIFNAYGPGQHLPPSHPPVVPHYLRQAQRGGTLVAHGDGSQSRDYIYVDDVVSA
                     MVAASTAPNINGAVINVGSGTETSVKDLIRIVLDVTDSKANVVYNPQTSGGVSRMKAD
                     LNLAKEKLRFTPSIKLEEGLRLTLQRDARFK"
     gene            4527..4739
                     /locus_tag="PL000_19820"
     CDS             4527..4739
                     /locus_tag="PL000_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNC91186.1"
                     /translation="MSDRIIGTVKWFNGDKGYGFLAREGGADVFVHFSAIQGDGYRNL
                     QEGQKVEFTVEKGPKGPQAANVTILA"
     gene            complement(4901..5251)
                     /locus_tag="PL000_19825"
     CDS             complement(4901..5251)
                     /locus_tag="PL000_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91187.1"
                     /translation="MPESEADRLKRLRERQLRDRDPLERERKFQRNSAIKEKRMRKPI
                     SLQEDWGKVSYTIKMPIYALLLGGIGTVILTKVWISPYAVYTGAGITIVLLIFGVLVG
                     NAVDLREDIKKHLK"
     gene            complement(5353..5805)
                     /locus_tag="PL000_19830"
     CDS             complement(5353..5805)
                     /locus_tag="PL000_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91188.1"
                     /translation="MKTYLLAWNPKRWAWKNLPAMAQQVKDGKMILDRWGTGSSQKLK
                     RGDRFFLIRLGENPKGIFASGEVMTDSFQDLHWDEQKALLGETANYVQIKYDILLDPD
                     HGPILPRELLVMPPLSAMHWEIRMSGIEIPAHIARELERLWAEFTEQL"
     gene            complement(5946..>6299)
                     /locus_tag="PL000_19835"
     CDS             complement(5946..>6299)
                     /locus_tag="PL000_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC91189.1"
                     /translation="ARGLDIETISHVINYDMPDTADAYIHRIGRTGRAQRTGDAFTLV
                     THEDNDMILILERIMGQRIKREYLEGFDYTRPAPPKTDSGRGRGAPRDDSRRPPRPAP
                     TPKSYGRNRLAPKKK"
BASE COUNT         1364 a         1601 c         1638 g         1596 t
ORIGIN      
        1 ttcaattcta ccatcccatt ttgaagcgcc ttttccccaa ccgtcacgcg gatcgggcag
       61 ccgatcaaat ctgcatcatt gaattttacg cctgcacgct cgtcgcgatc gtcgaataga
      121 actgagatcc ccgcattttg caattggttg tagatctttt cggcttcggc gcgggtatcc
      181 atttgctttc cgggtacatg catgagatac acatcaaacg gcgcagcaga tttgggaaag
      241 gtcaacccct tgtcatcatg atgccgctcg gcgagggcca gcagccagtt ttcatagtaa
      301 aggccgaagc gcgttcccaa cggcattgca gaaacaactt caagtgcatt tccgcaattt
      361 gagcaggtgt cgcctgcttt tgccattacc aggtcttcaa cgatctccgc cgaataatca
      421 cgcggataat ttgtgttcaa aagatgatat cccgcttcat tcgcgcctgc cgccagattt
      481 tgtgattggg gaatcaggtc atcaaccagg atgagagcat cgttgaggcc aatgggcgat
      541 gcataccccg ccaccgcacc tgaccttgaa attgcttcgg cgttagcagg ctgaatctcc
      601 ccgacgatat attccagttt ggcctcactc agttgcatat ctccgcgtac ggcggcaaag
      661 acaaattttc catcttcctt gcgggtgaac atcaatgcct tggcggtctt ctccttcggc
      721 aagccaagga aatttgccag cgattcgatg gtgttgcaat caggcgtcaa caccttttca
      781 agcggacgtt cctcttcgtg gggcaggggt gtcttcttga attttgccat gtccaacagt
      841 tcggtgtatt tgcaggaagg acaatgggca acttcgagat tgcccgtgac catcggaaag
      901 aaaaccgcct gctcgctgac cagccgcggc aggtcaacca cctctgaaaa tgaagggtcg
      961 ttcgagaggg tttccagccg tgtcagcgct tctttgccaa gcggcaggat ctcacattca
     1021 cgggtcatat atcccgcgcg gacaaggagt gcgaatccct ccgtccgtgc gttgttgggg
     1081 gcttcacgct gggtttggat tttcaggtcg gtatatttca tgggattaac gagggaggga
     1141 cacggcatgg ccgtgtccct atcactcggg tgaatttaga cttcgctttt tttcttcccg
     1201 ttgcgcatca acttttgggt tgaatctgtc gggagggtgg gttcgggggt ggaaatttga
     1261 attggcgttt gggcgatgga ttctgcctgg gcggcctctg aaggcgtgga ttcgggagca
     1321 ggctcaaggt ccagatcgac cgtttcttcc atccgaattt gcccgcgcag gaaggctcct
     1381 tcttcggtca cgaaagcggt tgtgaccaca tctccccata cccgacccga agcgcggatc
     1441 tcaagttttt gagtggtgat gttgccgcgc acagcccccg ctacgatgac ggtatttgcg
     1501 cgcacattct ggcaggtcac acgccctgtt tcgcccacga ccaacatgcc acgcagggcg
     1561 atttcgcctt caaacgcgcc ttcgatacgt acaccgcctg aaccgttgat gcttccgtgc
     1621 cagatcaggc ctgagccgag cacagaggtg attctttcaa cagcttgcac aggttggacg
     1681 gcttcacttg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncaattttac
     1801 cttgaaaacc ttaagcgcgg agcaggggta ggggtggagc aaacaggcaa aaaaaggcac
     1861 gcagaccttc ctgtccgcgt gcctgcttgc ttatgcatgt attgcttgct tctacccgcc
     1921 tttttcatcc ttttcactga cctgaacacc aatgatattg aacccggagt ccacgtacaa
     1981 tacttcaccg gtgatgtgtg cagaaaggtc agaagcaagg aaaacggccg agttccccac
     2041 gtcttcaatg gtgacgttct cgcgcatggg agccatatcg gcaaaatgct tgtacatatc
     2101 gcggaagccg cccacacctg ccgccgccaa tgtgcggatg ggtcctgccg agatcgcatt
     2161 gacgcggacc ttctggggcc cgaggtcata agccagatag cgggtggatg attccagtgc
     2221 tgccttggcg acacccatca cattgtagtg cggagcaacc ttcaccgagc cgtaataggt
     2281 catgcacatg accgaagcgc ccgggttcat gatcggcaga aaggcatggg tcaatgccac
     2341 aagtgaaaag gcactgatct ccaatgcggt tttgaagccc tcgcggctgg tttcgtggaa
     2401 aggcttgctc aattcatcac ggccagcata ggcaatggaa tgaaccagca cgtcgatctt
     2461 gccgaaatgt ttcgcggctt tttcaacggt ttgagcgata tggtcatctt ttgtaatatc
     2521 gcattcctca agccattgac aatccacctg ctcggccagc ggcttgacac ggcgttcaag
     2581 catttcacca gcgtagctga taccgatgtt cgcgccctcg cgtttaaaag cctgtgtgat
     2641 gccccacgca atggatttgt cgttcgcaag accaaaaata agtgcgttct ttccttccag
     2701 taatcccata tcaatctcct cgatattcga tattgtatac ccgactattc aatgtcggct
     2761 atcgtttctt tgaccaaaaa ccgccatggc ttggagaacc aaggctctgg cgtagtattt
     2821 aaacccacgc gggggccaat cgtcacgcat ttatcgggga ctttaaaccc cgcctcgatc
     2881 cttaaactgg aagctgattc tgtcagatcg gcatagttct cacttttggt gatccccagc
     2941 gcctgagtca gtttgcctgg cccaagcgta tcccgcccct gacgacgctc catcatcacg
     3001 tcaacgcctt ccaacggttg tatcgcgcgg atgagcaccg ccgcaggaaa gccttccttt
     3061 tcagtgaccg cgttcaacat ccagtgattg ccgtaggtga agtaaacgta ggccagcccc
     3121 ggcggaccaa acatcgggtc ggttcggatg gtgcgtcctg ccttggcgtg actggcaaga
     3181 tcgtcaaatc caaaataggc ctcggtctcg ctgataatcc caacaagttt catcccatcc
     3241 gagattcgca ccaggcgtgc cccgagcaat tcgcgggcaa cggtcagtgt gggtcggttg
     3301 tagaatttac gagggagggt cattgttact gtttgcaggt tggaaggttt acaatttaga
     3361 acgttcaaac cttccaacaa gaattttatt taaatcttgc atctcgctga agcgtgagcc
     3421 gcaatccttc ttcgagtttg atggaagggg taaaacgcaa tttttccttt gcgaggttca
     3481 aatctgcttt catgcgtgag acgccgccag aagtttgcgg gttatagacc acattggctt
     3541 tgctgtcggt cacatccaat acgatgcgga tgagatcctt gaccgaggtt tcggtgcccg
     3601 aaccaacatt aataaccgct ccattgatgt tcggcgcggt ggatgcggca accatggcgc
     3661 tgaccacatc atccacataa atataatcac ggctctgact gccatctcca tgggccacca
     3721 atgtccctcc gcgctgtgcc tggcgaaggt aatgcggcac cacagggggg tgggagggtg
     3781 gcaggtgttg tccggggccg taggcattga agatgcgcag gctgaccgtt tcgatcttcc
     3841 acagagaacc gatggtgttg acgtaatatt ccgccgcaag tttggaaacg gcatagggtg
     3901 aacgcgggtt gggtctggtg gactctgtca gtgtagagtc tcccaagtcg ccgtaaacag
     3961 caccggacga agccagaacg actcgtttga ctcccacatc gcgcatggct tccatcaagg
     4021 ccacggtccc gcccacattg acggcgttat agtcgcgtgg atataaaatg gattcctgga
     4081 ctgagacacg cgccgcaagg tgatagacca catccacatc ctggagcagg gtccacaact
     4141 tggggcggtc gctgacatct ccgcgtgtaa aatggacatc cggtgcaagc accttggggt
     4201 cacccgtgga aaggtcatca aggccgcgta cctggtgtcc ttcgcgggca agatggtttg
     4261 caagagaaga gccgaggaat cccgcggctc cggtgatcag aaaattcata ggaaaagatt
     4321 ataaccgcaa tattggtgcc tgtttttgat tgactcgtgt ttggtactgt gttatcttta
     4381 ctgccgatag gaaaacggta agttacgttg gtttggagaa catcaacagc tcacggtttg
     4441 accgtgggct gttttgtttt gacctccgat ggaacaaaaa cggcttgacc taatacaaat
     4501 tttttagttt gccaaaggag gcaaaaatgt ctgatcgaat aatcggtact gtaaagtggt
     4561 tcaatggtga taagggctat ggtttcctcg cccgtgaagg tggtgcggac gtgttcgtcc
     4621 atttctccgc cattcagggt gatggttatc gcaaccttca ggaaggccag aaagttgaat
     4681 tcaccgtcga gaagggcccc aagggtcctc aggcggcgaa cgtcaccatt cttgcttaaa
     4741 tcataaaaag cggacgatgc gaaagcatcg tccgcttttt gattccaatt tgtttatgct
     4801 tgaaagacgg gggaatatcc gcttttttgt gctttcatat cgtacaacag tatattggcg
     4861 cgttccgggc ttataagctg aaacgtctgc tgcttttgtg ttatttcaga tgcttcttga
     4921 tatcctcgcg caggtccacg gcgttgccga caagtacgcc aaaaataagc aggacaatgg
     4981 ttatgccagc gcctgtatag actgcatatg gcgagatcca aacttttgtg agaatgaccg
     5041 taccgattcc gccaagtaac aatgcataga tcggcatttt gatcgtgtaa gaaaccttgc
     5101 cccaatcttc ttgaagagag atcggcttcc gcatccgctt ttctttgatt gcggaattgc
     5161 gctgaaattt gcgttcgcgt tccagcggat cacggtctct gagttgcctc tctcgcagtc
     5221 ttttgagtct gtcggcttct gattctggca tgggcgtttt cctttcacta ttcagcagag
     5281 cctgattcga aaagatactt caattcatat tatatggcgg tgatgcgttt gtaagattaa
     5341 atttccaccc tgctaaagtt gttcggtaaa ctctgcccac agcctttcca actcgcgggc
     5401 gatgtgtgca gggatttcga tgcctgacat tcgaatctcc cagtgcatag ctgaaagcgg
     5461 cggcatgacc agtaactcgc gcggcaaaat cggaccgtga tcagggtcca acaaaatatc
     5521 atatttgatc tggacgtagt ttgctgtttc gcccaacaag gctttttgct catcccaatg
     5581 caggtcttga aaagaatccg tcatcacctc acctgaagcg aagattcctt tcgggttctc
     5641 acccagacgg atcaaaaaga atctgtctcc tcttttcagt ttctgcgatg accccgtgcc
     5701 ccagcgatcc agaatcattt tcccatcttt gacctgttgc gccattgcgg gcagattttt
     5761 ccatgcccag cgttttggat tccatgcaag tagatatgtt ttcatagatc ttttagcaga
     5821 cacccgcctc atatgaggcg ggtgtccatt cattgatgat cgatttactt cttttccgaa
     5881 aattgctcct gccgccgtcc tcggcggcgg ggacgccgcc ttgagcagaa tatttggata
     5941 ggttcttact tctttttggg tgcgaggcgg tttctgccgt agctttttgg ggtgggggcg
     6001 gggcgtggtg ggcggcggga gtcgtcacgg ggggctcctc ttcctcggcc agaatcagtt
     6061 ttcggcgggg caggacgcgt gtaatcgaat ccttcgagat attcgcgttt gatgcgttgt
     6121 cccatgatcc gttcgagaat tagaatcatg tcgttgtctt catgggtgac gagggtgaag
     6181 gcatcgcctg tgcgttgggc gcggcctgtg cgaccgatgc ggtggatgta ggcgtcagcg
     6241 gtgtcgggca tgtcgtagtt gatgacgtgc gaaatggttt cgatatccag cccgcgtgc
//
LOCUS       DAOQLC010000189         6133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5520_length_6133_cov_24.205989, whole genome shotgun sequence.
ACCESSION   DAOQLC010000189 DAOQLC010000000
VERSION     DAOQLC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5520_length_6133_cov_24.205989"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..474)
                     /locus_tag="PL000_19840"
     CDS             complement(136..474)
                     /locus_tag="PL000_19840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC91190.1"
                     /translation="MDKIALQSEQKGNVVVVSVSGRVDSDTAPMFEAELTKAVSGNAK
                     LVLDLKGVEYMSSAGLRAIVKAAQAVEPKGGAVKMAAVPELVTSIFYTVGLNQKVAEY
                     KSVEEAMASF"
     gene            complement(531..1055)
                     /locus_tag="PL000_19845"
     CDS             complement(531..1055)
                     /locus_tag="PL000_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNC91191.1"
                     /translation="MSKSDPNAPKRLALIASKGTLDWAYPPFILASAAGAMGWEVGIF
                     FTFYGLTLLKKDLSASVSPLGNPAMPMKMPFGPEGFQNIAWPMPNLLMANVPGFEALA
                     TNLMKQTFKNKGVASIEELRDMCIDLDVKMIGCQMTMDVFGFKKEDFIKESEVAGAAM
                     FLEYAADADVQLFI"
     gene            complement(1087..1326)
                     /locus_tag="PL000_19850"
     CDS             complement(1087..1326)
                     /locus_tag="PL000_19850"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNC91192.1"
                     /translation="MTDVIKEDQVLDCSGMLCPMPVVKTSKAMKELQSGQVLKMVATD
                     PGAPPDMEAWSRQTGNELLRSMTEGGKFIFYLRKK"
     gene            1456..3120
                     /locus_tag="PL000_19855"
     CDS             1456..3120
                     /locus_tag="PL000_19855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNC91193.1"
                     /translation="MITRVESFFRSNRVFVFLVIFRWASLLPALFTLGTTDALIHPLL
                     LLALALAINGIVALFNRPLNRMVVEKPALLSIDLLFSAFILAISGGSHSPYFLYALSP
                     LLAGAFFFQLRGALTTSLLFTILYSLAITYPAQPPADPTVLATQFAGIWVLPILFAYP
                     STLLKSVNQATEELSAAHRQLEIIHDLTVLLQAAPDLISVQERVLGAVVTDLGFSKAI
                     VAMVDPAHEEIGGWLIYPPENTFPQTKPLPLKSGSGEIIKALLNRNNLTTTGQTLIQD
                     PQLNHWLNQNQWFIVPLYLREHPVGVLLVEESGLTREKESTLMTVANQAALSLGTTIL
                     CIDRARRLAVETERNRIARDIHDTVAQSLFGIVYSLDACITMLPQQADDVKKELIDLR
                     TLANTAHDEVRRSIFDLWPSALNIELFMADLTNYVSGCCRPRSFSIIFNNHGDFNSLS
                     SGMRRTVYRMAQEALANSARHSGAQSARLCLNVTSDEVYLVVSDEGKGFDPSVALSRS
                     QNREHFGLHGIQERARAMGGDCEVISQPNAGARIMIQLPNNGHHHA"
     gene            3113..3763
                     /locus_tag="PL000_19860"
     CDS             3113..3763
                     /locus_tag="PL000_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91194.1"
                     /translation="MPESIRILIVDDHAVVRKGLAMVLRLESDLEVVGEAENGRIGLE
                     AAKNLNPDIVLVDLVMPEMDGQEMALALRKSNPKTKVMMLTGTEVDDRVFDLVAAGIE
                     GYVLKNIEPGELVRAIHAVVHGEAYLHPDVMKKVVGRMQPHHTQPIVLTPREIEILEW
                     MASSNTYKQIAGHLNVSEETIRSHAKNILEKMKQPNRAQAVLAAMRVGLIKLDESK"
     gene            complement(3770..5272)
                     /locus_tag="PL000_19865"
     CDS             complement(3770..5272)
                     /locus_tag="PL000_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91195.1"
                     /translation="MSKPNYPPEFETQVRQAMEVSEPKPETLDALRKQFIARGTSSLK
                     TDLQPDPAPNPFRPEKESNKMNRKPFRFSPRLAWSVALLILASLLVIAFSSPKVVTAL
                     RRLLGYVPGVGVVEQSPSLRMLAEPVTVTRESVTLTIDQAVLTNEKTVVVYSYVTPLY
                     NSDEGFFMDGPMGIPALVLPDGTRLEIVIGRQVSTMDCSECGDIRYAMEFPPIPADVQ
                     EAILEIPNLVGLQNGLTPLNWVIDLKFAPADPSTIIPVIEYQPTATPVSATSVSTTQT
                     PETYGAILVLDKSASLPDGYILYGTITWTDPIIPQYGLSATLAGIKDANGNDIPFEYA
                     DAEAYPQQGELREYWAYKIPQLDFAAPLTLSFAVNAWTPLTDAPSFTFDPGPNPQLGQ
                     KWEINQDVVVKDEIVHVLSVEQAGIEEGFFMFTMQSDSNIVGASITDLQHPPMGGGGG
                     GGGVPVVKLPFFVGFGYQTPIPTGPLTLTFQSVNVLLQGDWSVTWSPAAP"
     gene            complement(5265..5834)
                     /locus_tag="PL000_19870"
     CDS             complement(5265..5834)
                     /locus_tag="PL000_19870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC91196.1"
                     /translation="MSNQNMGRKDAPPNLGDALIQDAQHGEPAALAAIYDAYALRIHR
                     YFYSRVENAADAEDLAAQTFLSVIEALPHYQHRGQFTAWIFQVARSKAMDFFRRTHST
                     VQHEAEMVGSAFDDTLEQVIQGQDVERLRHLIRLLDDDERELLRLRFVVELSYVEIAE
                     LLGRKEDAVRKSVNRTLERLHLLMEAKHV"
BASE COUNT         1518 a         1679 c         1463 g         1473 t
ORIGIN      
        1 gcagtcgcca aaaagaaagg cgttgccgtt cgttcaggat ccttctcccc gtgagtctga
       61 acgtacaaaa ttctctttgt ttcaggatca ttcgtcttcc aaacgctcat actgccctct
      121 cctatttatc tgaaattaga aactagccat tgcttcttca acagacttat attcagcgac
      181 tttctggttt aaaccgacgg tataaaaaat ggaggtaacc aactcaggca cagctgccat
      241 ttttaccgct ccacccttcg gctccacagc ctgagccgct tttacgatcg cgcgaagccc
      301 tgcagacgac atatattcca cgcccttgag atccagaaca agctttgcat tcccacttac
      361 cgctttggtt aattcggctt caaacatggg ggctgtatca gaatccaccc gtccagaaac
      421 agataccaca acaacatttc ctttttgttc agattgaaga gcgatcttat ccatgcattt
      481 atcctttttt aagtaggtca cgccttatgc gtgacctaca gggtaatcta ctagataaac
      541 aattgaacgt ccgcgtcagc ggcgtactca aggaacatgg ccgcaccagc tacttcactt
      601 tccttaataa aatcttcctt cttgaagcca aagacatcca tcgtcatctg gcagccgatc
      661 atcttcacat caagatcaat gcacatatcg cgaagttctt caatcgaagc gacgccctta
      721 ttcttgaagg tttgcttcat caggttggtc gccagtgctt cgaacccagg cacattcgcc
      781 atcaacaaat tcggcatggg ccaggcaatg ttctgaaaac cttcgggacc aaagggcatc
      841 ttcatcggca tggccgggtt ccccaacggt gagacagatg cgctaagatc cttcttaagc
      901 aaagtcaaac cataaaaggt aaagaaaata cccacctccc accccatcgc ccctgccgca
      961 ctggcaagga tgaatggagg gtaagcccaa tccagcgttc ccttgctggc gatcaaggca
     1021 agtctctttg gagcatttgg atcagatttt gacataggga cctctattaa gaatttggaa
     1081 caagttctat ttcttgcgca ggtaaaaaat gaatttaccg ccttcggtca tggaacgcag
     1141 tagttcgttg cctgtctggc ggctccaggc ttccatatcg gggggagcac cagggtcggt
     1201 ggcaaccatc ttcaaaacct gtccagattg caattccttc attgcttttg atgtcttcac
     1261 cacaggcatg gggcaaagca ttccagagca atccaaaacc tgatcttcct taattacatc
     1321 tgtcatttgt gcctcctttg gaactcataa atcataatga attgaccgcc gatatgccat
     1381 cccacacgca agggatttta tctcccccgc gcgggggaga tggccaattc ggatataatc
     1441 aattctacaa cttttatgat tacaagagtc gaatcgtttt tccgatcaaa ccgcgttttt
     1501 gtatttcttg tcatctttcg ctgggcttcc ctgttaccag cgttgtttac gcttggaaca
     1561 acagacgcat tgatccatcc gttgttgttg cttgcactcg cgcttgccat caacggcatc
     1621 gtagctctgt tcaaccgccc attgaacagg atggtggtag aaaaacccgc cctcctcagc
     1681 attgacctgc tcttctccgc cttcatcctc gccatcagcg gcggatcaca cagcccttac
     1741 tttctgtatg cgctcagccc attactggct ggcgcattct tttttcagct tcgcggcgca
     1801 ctgaccacat ccctgctttt tacaatttta tattcccttg ccatcaccta ccccgcccaa
     1861 ccgcctgcag accccaccgt attggctact caatttgcag gcatttgggt tttgcccatc
     1921 ctctttgcat atccctccac gctgcttaaa tccgtcaacc aggccactga ggaactctca
     1981 gcagcacacc gtcaactgga gatcattcat gacctcacag tgttgctgca agccgcacca
     2041 gacctgatct cagtgcagga acgtgtcctc ggcgcagtgg tgaccgacct tggtttctca
     2101 aaagccattg tcgccatggt agaccccgca catgaagaaa tcggcggttg gctcatctac
     2161 ccacctgaaa acaccttccc gcaaaccaaa cccctccccc tcaaatcagg aagcggcgag
     2221 atcatcaaag cccttctcaa tcgcaataac ctcaccacaa caggccagac cctgattcaa
     2281 gacccccaac tcaaccactg gcttaaccaa aaccaatggt tcatcgtacc gctttatttg
     2341 agggaacacc ccgtcggagt attgctcgtg gaagaatctg gacttaccag agaaaaagaa
     2401 tccacgctga tgactgtggc gaatcaagcc gccctgtcgc ttggtaccac catcttatgc
     2461 attgaccgcg cccgacgcct cgccgtcgaa accgaacgca accgcatcgc ccgcgatatt
     2521 catgatacgg tcgctcaatc gctcttcggc atcgtttatt cgctggatgc ctgcatcacc
     2581 atgctgcccc aacaagccga tgacgtaaaa aaagagttga tcgacctgcg taccctcgcc
     2641 aacaccgccc acgacgaagt ccgccgctcg atctttgacc tgtggccctc tgccttgaac
     2701 attgagctct tcatggcaga cctgacgaat tacgtcagtg gctgctgccg cccacgttct
     2761 ttcagcatta tcttcaacaa ccacggcgat ttcaattccc tctcttcagg catgcgtcgg
     2821 acagtttacc gaatggcaca ggaggcgctg gcaaattctg cacggcactc gggggcgcaa
     2881 tcagccagat tgtgcttgaa tgtaaccagc gacgaagtgt atctcgttgt ttcagatgaa
     2941 ggcaaaggct tcgacccatc tgtggcgctt tcacgaagcc aaaaccgcga gcattttggt
     3001 ctgcacggaa ttcaggaacg tgcccgcgcc atgggtggtg attgcgaagt catctcgcag
     3061 cccaacgctg gtgcgcgcat catgatccaa ctccccaaca atggacacca ccatgcctga
     3121 gtcgatccgc attctcatcg tagatgacca tgccgtggtc cgcaaagggt tggcaatggt
     3181 ccttcgtctg gaatcagatt tggaggtcgt aggcgaagcg gaaaatggtc ggatcgggct
     3241 ggaggcggcg aagaatctaa atccagatat cgtccttgta gatctggtca tgcctgagat
     3301 ggatggtcag gaaatggctc tcgctttgcg aaaatccaac ccgaaaacaa aggtaatgat
     3361 gctgacaggc accgaagtgg acgaccgtgt ttttgacctg gttgcggcgg gcattgaagg
     3421 ctatgtgctg aaaaatatcg aacctggtga attggtgcgc gccattcatg cggtggtgca
     3481 tggcgaagca tatttgcacc ctgatgtgat gaagaaagtg gtgggccgca tgcagccgca
     3541 ccatacacaa cccatcgtgt tgaccccgcg cgagatcgag attcttgaat ggatggcttc
     3601 ttcgaacact tataagcaga ttgcggggca tctaaatgtc agtgaagaga ccatccgaag
     3661 ccatgccaag aacatcctcg aaaaaatgaa acaacccaac cgcgcgcagg ccgttttggc
     3721 cgccatgcgg gttgggttga tcaaattgga cgagagcaaa tagccgtttt tacggtgccg
     3781 caggcgacca ggtgacagac caatcgccct gaagaagcac attcaccgat tggaaagtca
     3841 gtgtaagcgg cccggttgga atgggggttt gataaccaaa accaacaaag aaaggcagtt
     3901 ttacaacagg cacacctccc ccaccacctc caccgcccat gggcggatgc tggaggtcag
     3961 tgatgctcgc gccgacaatg ttcgaatcag actgcatggt gaacatgaaa aatccttctt
     4021 caattcctgc ctgttctaca gagaggacat ggacaatttc atccttaacc accacatcct
     4081 gattgatctc ccatttctgc cccaattgcg gatttggccc cgggtcaaag gtgaaagagg
     4141 gtgcatccgt caaaggggtc catgcgttca ctgcaaaaga taaagtcaac ggtgcggcga
     4201 aatccaactg cggtatcttg tacgcccaat attcgcgtaa ttctccctgc tgtgggtatg
     4261 cttccgcatc ggcatattcg aaggggatgt catttccgtt cgcatccttg atgcctgcca
     4321 gggtggcaga caaaccatat tgggggatga tgggatcggt ccatgtgatg gtgccataca
     4381 aaatatatcc atcaggaagc gacgcggatt tatcaagcac aaggattgcg ccataggttt
     4441 cgggcgtctg ggttgttgaa acactcgtcg cgctgacggg cgtcgctgtc ggctggtatt
     4501 cgatgacagg aatgatggtg gatgggtcgg caggcgcaaa cttcagatca atgacccaat
     4561 tcaagggtgt caggccgttt tgcagcccaa ccaggttggg tatctcaagg attgcctctt
     4621 gaacatcagc ggggatgggc ggaaactcca tcgcatagcg gatgtcgcca cactcagaac
     4681 aatccatcgt actcacctgc cgtccaatta cgatttcgag tcgggtgcca tcgggcaaaa
     4741 ccagtgcagg gatgcccatt gggccatcca taaaaaagcc ttcatcagag ttatacaaag
     4801 gtgtcacgta ggaatacacg acaactgtct tttcattggt cagaacagcc tgatcaatgg
     4861 tgagggtcac actttcgcgc gtcacggtga cgggttcagc caacatgcgc agagatgggc
     4921 tttgctccac cacacccaca ccggggacat accccagcag gcggcgcaag gcggtcacta
     4981 ctttgggaga tgagaatgcg atcacaagca ggcttgcgag gatgagcagc gccacgctcc
     5041 atgcaaggcg cggggaaaag cgaaatggtt tgcgattcat tttgttcgat tccttttcgg
     5101 gtcgaaaagg attgggagca ggatcaggct gaaggtccgt tttgagcgag gaggtcccac
     5161 gagcaatgaa ctgtttgcgc agggcgtcca atgtttcggg ttttggttca gacacttcca
     5221 tcgcctgccg cacctgtgtt tcaaattcgg gcggataatt gggcttagac atgtttggcc
     5281 tccatcagca ggtgcaaccg ttcgagcgtg cggttcacag attttctgac ggcatcttct
     5341 tttcgtccaa gcagttcggc gatctcaaca tacgagagtt cgaccacaaa acgcagacgc
     5401 agcaattcgc gctcgtcgtc atccagaagt cggatgaggt gacgaagacg ttcgacatcc
     5461 tgaccttgaa tgacctgttc cagtgtgtca tcgaaagcac tgcccaccat ctccgcttca
     5521 tgctggaccg ttgagtgggt gcggcggaaa aaatccattg ccttgcttcg agcgacttga
     5581 aagatccacg cggtgaattg accgcggtgt tgatagtgcg gcaaggcctc aatgacactg
     5641 aggaaggtct gtgcggcaag gtcttcagca tcagccgcat tttccacacg gctgtaaaaa
     5701 taacggtgga tgcggagcgc gtacgcgtca taaatcgccg ccagtgcggc gggttcgccg
     5761 tgctgtgcat cctgtatcag cgcatctccc aggttgggag gcgcatcttt tcgacccatg
     5821 ttttgatttg acattcgata ccctttgcaa aggaaactct ataatattaa acgcaaaacc
     5881 ttcaaaaagc ggacaaaatg ggtatccaga tttcagcccc tgtctgttgc ctcggcaaaa
     5941 attccctcct ccagccatct tttactacgg caggatgatt ttttcaaaaa tattcaaaac
     6001 ctccctgggg ggtgaatcca gtttttcaag aatacggaga acaaagaagg tttcaagctg
     6061 ttccgtgcca tcttcaaatt gccaaatact ttcagagcga ataagcctcc cagtttgaaa
     6121 gttcatccaa atc
//
LOCUS       DAOQLC010000190         5961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5773_length_5961_cov_16.771419, whole genome shotgun sequence.
ACCESSION   DAOQLC010000190 DAOQLC010000000
VERSION     DAOQLC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5773_length_5961_cov_16.771419"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="PL000_19875"
     CDS             <1..1505
                     /locus_tag="PL000_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91197.1"
                     /translation="ATLTIKYPGMTGIHNYVKMPDAISNSATGGAVEQRTWQNNESVM
                     TVLRGFYDVLIVKGSSSFIYDDVNCTALTCTLDKANLTVKFPGITSVHTYVRKSDNAA
                     GTATGTQVASQTYKTDQAVFADLPNGLYDVIVVKGAQTKIIDNVTVLGGWATVDKIVA
                     TLTVKFPGIVGVHTYAKVNDGVVGAAAGGSVEDRTYKTDETSMTVLKSTYDVIVVKGA
                     KQKVIDAVDCTGETCLVENIVATLTVKFPGIAGVHAYAKVNDGVVGTATGGSVEDRTY
                     KTDETSMTVLKSTYDVIVVKGAKQKVIDAVDCTGNTCVVENIVATLTVKFPGIISVHT
                     YAKVDDGVAGTAAGGSVEERTYKNNETSMVVLKGTYDVIVVKGAKQKVVDTVDCTGNT
                     CVVENIVATLTVKFPGLSSVHTYVKVNDGVVGTAAGGSVDERTYKTNEVSIVVLKSTY
                     DVVVIKGSKQVIVDAVDCTGDTCVIENILAALTAKFPASGLFAYNNKERR"
     gene            complement(1550..>4469)
                     /locus_tag="PL000_19880"
     CDS             complement(1550..>4469)
                     /locus_tag="PL000_19880"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC91198.1"
                     /translation="FFILHNSSFLMRFTSPLYLLLLLLVPITVWLGYPAAGQSRKRDL
                     TSLILRLVIVLCLILAISGLEIVQRGDNLAVVFLVDVSDSMPPQAVDSEVGYIREALQ
                     SIGADDQAAVILFGADALVERPMSALDELAEITSVPITNQTDLAEAIQLGLAMFPSGY
                     AKRMVILSDGAETTGNAAKAAEFAAASDVQIVVVPFVNQVDAEALVTEVDAPTHLRSG
                     EKFDLNVSIQANQSMQATVRVFSQTEMIYEGGHDLHKGLQTLSLPLTAQDPGFVTYQV
                     QVTPQQDAFYQNNRLDTFSQVEGPPRILVVSPLEGEAIPGGEIRPDETSLLLTSLTSA
                     GFDVRTVTPNLLPADLPTLAQYNSVVLVNVPARQLGRNQMETLQSYVRDLGGGLVAIG
                     GPTSYGVGGYYGTPLEEALPVDMQIKDEKRRPSLTMVFIIDHSGSMSDTSGGVTKLEL
                     AKEAAARSVELLFPNDHVGVIAFDDTASWVVPITDLQNPAEVVNAIGTIQIGGGTDIY
                     AGLLAMSETLPNDPSQTKHVVLLTDGGADITGIPELVKKLYEENGITLSTVGVGNDAA
                     PFLKDIAALGGGRYHFTNNASTIASIFTEETTLATRAYIVEESFFPTLVNSSPILANI
                     TEVPPLHGYVASSAKDLAQVILESEKGDPILAAWQYGLGKSVAFTSDATGRWARDWAR
                     SEIFPTFWVQAVRYTINDSLNSALQMNIEMQGENASITLDAYDRTGGFLNGYQVEASI
                     VSPNGREAAQTVTFTQTAPGRYESTFTPTEQGIYLIHFSGTTDSGNGSASFAETTGWA
                     LSYSPEYQRIESNPDLLLRLSALANGQVASPLPADVFKHDLQAARATQPMAPFLLLLA
                     ALLLPVDIAVRRLIVTKTDLIRLREWVTARFTKTSRAAEPINEKASPQMEALFSAKSR
                     VRENKPAQNEVKPSAQAMPVETESGSGPEKEAQETPPKAEQSASTTSVLLARKKNIRK
                     "
     assembly_gap    4470..4569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4570..>5961)
                     /locus_tag="PL000_19885"
     CDS             complement(<4570..>5961)
                     /locus_tag="PL000_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91199.1"
                     /translation="LKAALNKAQVTQGSADWEAAFALAAGATRNSASPTIVIISDGGL
                     PESGLPSLPGEVRYIPIGTGNDNIAISALALRASNGIPQLFAEVANYSNTNRTVLLSL
                     YRGDELLDARQLKVASDSKVGISMENLPATTAVYKAQISDPANANAKLDSLSQDDTAF
                     AIYQSSAARRVLLVSKGNLFLEQLLASLPGIQPFRALPADDGTLQIPNDPFDLYVFDG
                     YIPAELPKSNLLLINPPTNPFFEVGDAFKEMGEFQVNEHALTQFVDWKTVHVLQAHKV
                     SLPNWMNVLITTDDNPLVFAGETNGQRIAALSFDLRESDLPLQIAYPILFSNLINYLA
                     PPSAFDSSQALQAGESLSIVPQTDAKQVIVAAPSGTLYSLPPGQNLFSNTDELGFYAV
                     NFVTETSTRSEYFAVNLFDEAESNIQPRESLQLGSTSVTPTVSQQIGLQELWKWLAGF
                     ALIVLMIEWQVFH"
BASE COUNT         1341 a         1406 c         1746 g         1368 t
ORIGIN      
        1 ttgccacatt gaccatcaaa taccccggca tgacgggaat ccataactac gtcaaaatgc
       61 ccgacgcgat cagtaattcg gcaacgggtg gagccgtcga gcaacgtaca tggcagaaca
      121 acgaatctgt catgaccgtt ttgcgtggct tttatgatgt gttgatcgta aagggcagca
      181 gttcgttcat ctacgatgat gtgaattgca cagccctgac ctgtacgttg gataaagcca
      241 acctcacggt gaagttccct ggaattacca gtgtgcatac ttatgtacgc aagagcgaca
      301 atgctgcggg cacggcgacc ggaacacagg ttgcgtctca aacctataag accgatcagg
      361 ctgtctttgc cgatctgccg aacggtcttt atgatgtgat cgtggtaaag ggcgcacaga
      421 ccaagattat tgataatgta accgtacttg gtggttgggc taccgtggat aagatcgtag
      481 ccacactgac ggtgaaattc cctggtattg tcggcgtcca tacctatgcc aaggtgaatg
      541 atggcgtagt tggcgcagcc gcaggcggta gtgttgaaga ccgcacctac aagaccgatg
      601 aaacttcgat gaccgtattg aagagcacct acgatgtcat cgtggtcaag ggtgcgaagc
      661 aaaaggtcat cgacgcggtg gactgcacgg gcgaaacctg cctggtggaa aatatcgttg
      721 ctacactgac ggtgaaattc cccggtattg cgggcgtcca tgcctatgcc aaggtaaatg
      781 atggcgtagt tggcacagcc acaggtggca gtgttgaaga ccgcacctac aagaccgatg
      841 aaacttcgat gacggtgttg aagagcacct acgatgttat cgtggtcaag ggcgcaaaac
      901 agaaagtgat tgacgcagtg gattgcacag ggaatacctg cgtagttgaa aatattgtcg
      961 ccacgttgac agtgaagttc cccggtatca tcagtgttca cacgtatgcc aaggtggatg
     1021 atggtgtggc tggcacagcc gcaggcggca gtgttgaaga acgcacctat aagaataacg
     1081 aaacctcaat ggtggtactg aagggcactt acgatgttat cgtggtcaag ggcgcaaaac
     1141 aaaaggtcgt tgatacggtg gattgcactg ggaatacctg tgtggtcgaa aacattgttg
     1201 ccacactgac ggtgaaattc cccggactca gcagtgtcca tacctatgtc aaggtaaatg
     1261 atggcgtggt cggcacagct gcaggcggca gtgttgatga acgcacctac aaaaccaatg
     1321 aagtttcgat cgtggtgttg aagagcactt acgatgtggt tgtgatcaag gggtcaaaac
     1381 aggtcattgt ggatgcagta gactgcacag gcgacacctg tgtaattgaa aacatccttg
     1441 ctgcattgac tgcgaaattt cctgccagtg gtctctttgc ctataacaac aaagaacgac
     1501 gctagctcga ttgcttgaaa aatgtgcccc gaattttcgg ggcacatttt tattttcgaa
     1561 tattcttttt tcttgcgagt aatacagacg tggtcgatgc ggattgttct gccttcggcg
     1621 gagtctcctg cgcttctttt tctggacctg aacctgattc agtttcaaca ggcattgcct
     1681 gcgcggatgg tttgacctca ttttgtgcgg gtttgttttc ccgcacacgg ctcttcgcac
     1741 tgaacaaggc ttccatttgc gggctggctt tttcgttgat cggctccgcg gcccgactgg
     1801 ttttcgtaaa tcgtgccgtg acccattcac gcaggcgaat cagatcggtc tttgtgacga
     1861 tgagtctgcg gacggcgata tccactggaa gaagcaaggc ggcgagcagc agcaaaaatg
     1921 gggccatcgg ttgtgtggcg cgggccgctt gcaggtcatg cttgaacacg tctgcgggga
     1981 ggggagaagc aacctgcccg ttggccaatg ccgatagacg caggagcagg tcagggtttg
     2041 attcgatgcg ctggtattcc ggtgagtagg ataatgccca cccggtggtc tctgcaaaag
     2101 aggcggatcc gtttccagag tcagtcgtcc ccgaaaaatg gatgaggtaa attccctgct
     2161 cggtgggtgt gaacgtggat tcgtaccgcc cgggcgcggt ctgcgtgaat gtgacggttt
     2221 gcgccgcttc gcggccattc ggtgaaacga tgctggcttc cacttgatag ccgttgagga
     2281 acccgcctgt gcggtcatac gcgtcgaggg tgatgctggc attttcaccc tgcatttcga
     2341 tgttcatttg cagcgccgaa ttgagcgagt cgttgatggt gtagcgcaca gcctgcaccc
     2401 agaaggttgg gaaaatttca gagcgtgccc aatcgcgggc ccagcggcct gttgcatcag
     2461 aagtgaatgc gaccgatttc ccgaggccgt attgccatgc ggcgaggatc gggtcgcctt
     2521 tttctgattc gaggatgact tgtgcgaggt cttttgcgct ggaggcgaca tagccatgca
     2581 aaggaggtac ttcggtgatg ttggcgagaa tcggcgacga attgaccaaa gttgggaaaa
     2641 acgattcttc aacgatgtag gcacgtgtgg ccagcgtggt ttcttccgta aagatcgaag
     2701 cgatggtgct cgcgttgttg gtgaagtgat aacggccgcc gccaagcgcg gcgatatctt
     2761 tcaggaaggg tgcggcatcg ttaccgacgc ccacagtgga aagcgtgatg ccgttttctt
     2821 cgtacaattt tttcacgagt tcaggaatgc ccgtgatgtc ggcgccgccg tcggtaagca
     2881 ggacgacatg ttttgtttgt gaggggtcgt tgggcagcgt ttcagacatg gccagcaaac
     2941 ctgcataaat atcggtgccg ccgccgatct ggatcgtgcc aatggcattg acaacctcag
     3001 cggggttttg caggtcggtg atcggcacca cccaactggc ggtgtcgtca aatgcgatca
     3061 cgccgacatg gtcattgggg aaaagcaatt caaccgaacg cgccgcggct tccttggcaa
     3121 gttcgagttt tgtcacaccg ccgctggtgt cgctcatact tcctgaatgg tcaatgataa
     3181 agaccatcgt gagagagggg cggcgctttt catctttgat ctgcatgtct acaggcaggg
     3241 cttcttcgag cggcgtgcca taatacccgc cgacgccgta actggttggc ccgcctatcg
     3301 ctacaagccc gccgcctaga tcgcgcacat aggattgcag ggtttccatt tggttgcggc
     3361 caagctgcct tgcagggaca ttcaccagca cgaccgagtt gtattgagcc agtgtgggca
     3421 ggtcagcggg tagaagattt ggcgtgacgg ttctcacgtc gaaacctgcc gatgtgagtg
     3481 aagtgagtaa caatgaagtt tcatcggggc gaatttcgcc gcccggaatg gcctctcctt
     3541 cgaggggtga taccaccaaa atgcgcggcg gaccttcgac ctgcgagaac gtatccagcc
     3601 gattgttttg atagaaggca tcctgctgcg gcgtgacctg cacctgataa gtgacaaagc
     3661 cagggtcttg tgctgtgagc ggcaggctca aggtttgcag acccttgtgc aggtcatgcc
     3721 caccttcgta gatcatttcg gtttgcgaaa acacgcgcac tgtggcctgc atggactgat
     3781 tggcctggat cgaaacattc agatcaaatt tctcaccgct gcgcaaatgg gtcggcgcat
     3841 ccacttcggt gacgagggcc tcggcgtcaa cctgatttac gaacggcacg acgacgatct
     3901 gcacatctga agcggccgcg aattcggcgg ccttggccgc attgccagtg gtttccgcgc
     3961 catcagagag gatcaccatg cgttttgcat agcccgacgg gaacatcgcc agcccaagct
     4021 gaatcgcctc tgccagatcg gtttggttgg tgatcggcac ggaagtgatt tccgcgagtt
     4081 catccagcgc cgacattggg cgttccacca atgcgtctgc gccgaagaga atcaccgcag
     4141 cttgatcgtc cgcgccgatg gattgcagtg cctcacgaat gtagcccact tctgaatcca
     4201 cggcttgcgg cggcatggaa tcggatacat ccacaaggaa gacgactgca aggttatctc
     4261 cacgctggac gatttcaaga ccagagatgg cgaggatcaa acagaggacg atcacaagcc
     4321 gcagtatcag cgacgtgaga tcgcgcttcc gtgattgacc tgcggcagga taccccagcc
     4381 agaccgtgat cggcacgaga agaaggagca ggaggtagag cggagaggtg aaacgcatga
     4441 gaaaggatga attatgaagg atgaaaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnng atggaaaacc tgccattcga tcatcagtac gatcaaggca aatcctgcca
     4621 gccatttcca taattcttgc aggccgatct gttgagagac ggtgggtgtg acggatgtgc
     4681 tgccaagctg caggctttcg cgcggctgaa tattcgactc ggcttcgtcg aacaggttga
     4741 cggcgaaata ttcggagcgg gtggaggttt cggtgacgaa attcacggcg taaaagccga
     4801 gttcatcggt gttgctgaac aggttttgcc cgggcggcag ggagtaaagt gtgcccgatg
     4861 gcgcggcaac gatgacctgt tttgcatcgg tttgagggac gatggagagg ctctcgcctg
     4921 cttgaagcgc ttgcgatgag tcaaaggcgc tgggcggtgc aagataattg atgaggttgg
     4981 aaaaaagaat cgggtaggcg atctgcaagg gcaggtcact ttcgcgcaga tcgaatgaca
     5041 gggctgcgat tctctgaccg tttgtttcgc ccgcaaacac cagcggatta tcgtcggttg
     5101 tgatcagcac gttcatccaa tttggcagag acactttgtg agcctgcaac acgtggacag
     5161 ttttccaatc tacgaactgg gtgagcgcat gttcattgac ttgaaattcg cccatttctt
     5221 tgaaggcatc tccaacctca aaaaaaggat tcgtcggcgg gttgatgagc agcaaattcg
     5281 attttggcaa ctccgctggg atgtaaccat cgaagacgta tagatcaaac gggtcgttgg
     5341 ggatttgcag agtcccatcg tctgcgggca gggcgcggaa gggttgaatg ccagggagtg
     5401 aagccaataa ttgctcaagg aatagattcc cttttgaaac caacagcacg cgtcttgcgg
     5461 cggaggattg ataaattgca aaagcggtat cgtcttgtga gagggaatcc agttttgcat
     5521 ttgcgttggc agggtcggaa atctgcgcct tgtatactgc tgttgtcgct gggagatttt
     5581 ccatcgaaat accgactttg gaatcggaag ccacttttaa ttgacgggca tccagcaatt
     5641 cgtctccgcg atagagcgaa agtaaaacgg tgcggttggt gttgctgtag tttgccactt
     5701 ctgcaaacaa ctgcggaatg ccgtttgacg cgcgtaatgc cagcgcggaa atggcgatgt
     5761 tgtcattgcc cgttccgatg ggaatgtagc gcacttcgcc aggcagggag ggcaatccac
     5821 tttcggggag gccgccgtca gagatgatga cgatggttgg ggatgcgctg ttgcgggttg
     5881 cgcctgcggc cagcgcgaat gcggcttccc agtctgcgct tccttgcgtg acctgtgctt
     5941 tgttcagcgc tgctttgagc a
//
LOCUS       DAOQLC010000191         5920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5855_length_5920_cov_19.985166, whole genome shotgun sequence.
ACCESSION   DAOQLC010000191 DAOQLC010000000
VERSION     DAOQLC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_5920_cov_19.985166"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PL000_19890"
     CDS             <1..1277
                     /locus_tag="PL000_19890"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91200.1"
                     /translation="GYTISGDAGIGGATLTYTDGTVKTATADSSGLYSFQVPSGWSGT
                     VTPSKSGYEFSPTSRTYTSVGADQTAQDYTASVPIINLLQDPSFEAYTPNTYWLETST
                     NFGTPLCTIADCTNGAGTAGPRTGSVWAWFGGTTKDEAASLTQTVVFPTGTANLRFYL
                     WIGRADSGRGAADQFTVTVDGVVVFTADATEMGAYSSYTMVNLDVSTFADGAAHDIGF
                     SANTSGQMVNFNLDDITLAEAIPTFSDVPLDYWARSYIERLYNSGITRGCGSNPLTYC
                     PLDSVTRTQMAIFILRGIHGSGYTPPEASGTVFSDVTTSTFGAAWIEQFALEGITSGC
                     GSGKFCPNDYVTRAQIAIFLLKGKHGSTYVPPAATGDFTDVPVGSFAADWIEELAAEG
                     ITSGCGGGNFCPSQNVTRDQMAVFLVKTFNLP"
     gene            1428..3359
                     /locus_tag="PL000_19895"
     CDS             1428..3359
                     /locus_tag="PL000_19895"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91201.1"
                     /translation="MSAKSPVFRILLGMLVFSLIFSAVDVDVRAAPLADPVLDWSTFL
                     GGNGGDYGQAMAVDVTGSIYVVGYSDSDWGSPIRAFDSANDAFVAKFDSSGNLLWNTF
                     LGGGGDDVGINIVTSGNSIFVAGYSSSDWDCAPLTCTMDDYTLGYDAFVAKLDSSGNL
                     NWITFLGGNGSDFGRGIDVDFSGNIYVVGGSDGTWGSPIQSHAGGGYDGFAAKLDSTG
                     ARTWNTFQGSGGDDQTWGVALDVSGNAYLAGYSNDTWGSPVIAYDADYDGFAAKLNSN
                     GALIWNTFLGGNGEEHSHAIALDGSGTIYVTGYSTDTWGSPVRAYTSNDVFAAKLTSS
                     GALTWNTFLGGAGADAGYALALSGGDIYVTGYSTDTWGSPDRAYTSGFDSFAAKLNTS
                     GVLTWNTFLGGSGSDFSYGVAQSGSGLFVGGHSDDTWGTPLQAYDTAVDSFLVKFAGE
                     TTPPPASPIFADVPFDHWANPWVETLYNAGVTGGCATAPHLIYCPDDAVTRAQMAIFI
                     LRSMHGGAYLPPAATGAVFGDVPADAFAADWIEQLAAEGVTTGCGDGNYCPEAAVTRA
                     EMAIFLLRGKYGGAYTPPAATGTVFGDVPSDSFAADWIEQLASEGITVGCGDGNYCPD
                     MNVTRAEMAVFLVKTFHLP"
     gene            3461..4873
                     /locus_tag="PL000_19900"
     CDS             3461..4873
                     /locus_tag="PL000_19900"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HNC91202.1"
                     /translation="MKTIKFLWVTLVASMLLFALMLPGGKAKSNGMQQAPIGVGGTAW
                     NLIWSDEFDGSAVNSNDWNFNVGGEGWGNNELEYYTDGQNVTVANGELVITAKNENGG
                     YACWYGPCEYTSTRMTTKAKQEFQYGRVEARMKLPNGQGLWPAFWMLGSNFDQVGWPA
                     SGEVDVMEHINSEDIVYGTMHWDAGGYASYGCVSTDVLASVDVTQYHVYAIEWDTEKI
                     VWYVDDTQYCEANIRDSINSTEEFHAPFYILLNLAVGGNWPGAPDAGTAFPASMAVDY
                     VRVYSADVTSIFTDVPPTYWAWNFIERLYNAGITGGCSTAPLMYCPEATVTRAQMAIF
                     ILRGMHGSAYVPPAATGTVFADVPLGAFAADWIEQFAAEGVTAGCGGGNYCPDATITR
                     AQMAIFLLRGEHGSAYVPPVATGAVFGDVPAGSFAADWIEQLAAEGITSGCGGGNYCP
                     DSPVTRAEMAVFLVRAFSLP"
     gene            5007..>5920
                     /locus_tag="PL000_19905"
     CDS             5007..>5920
                     /locus_tag="PL000_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91203.1"
                     /translation="MSTKTVFRAFSVLFVFVFLLAQVGGAGAISPMANSVVLTPGRAI
                     ASWPSGVPAGEKRPVVVFLPGWGGSGAVDAAVSAQNTNLVNQGYVTLAIGFNSPSDWS
                     SDIDVQTADGLDKLCADASIPANCDAIILDGESYGGAQNYYVIEYIRSNGYGGKALGF
                     VSEDAGYAAPGNLIDPVTGEFQRTGLADTASYGVAMIQNLGDSTFPVDDCTWGNCGAR
                     TLSDAHQAQGDTNVFSICPSGGEHGTRGYADWDAWVVSAIKTLFHSVYGVPTFTGYTA
                     PTISVGNTCLSSGGSSTFVDVPTTHPLY"
BASE COUNT         1267 a         1348 c         1712 g         1593 t
ORIGIN      
        1 cagggtacac catttcaggc gatgcaggca tcggtggcgc taccctcacc tataccgatg
       61 gaacagtgaa aaccgctact gcagatagta gtggcttgta ttcgttccag gtcccttcag
      121 gttggtctgg tacggtgaca ccaagcaagt cggggtatga gttttcaccc acgagcagaa
      181 cttatacgag tgttggcgca gaccagacag cccaggatta cacggcttct gtgccaatta
      241 tcaatctgtt acaagatccg agctttgaag catatacccc caatacatat tggctggaga
      301 cctcaacaaa ctttggcact cccttgtgta ccattgcaga ctgtaccaac ggtgccggta
      361 ctgccgggcc gcggacgggt tctgtatggg catggtttgg cggtacaaca aaagatgaag
      421 ctgcatcgtt gacgcaaacc gtggttttcc ccactggcac tgccaacctt cggttctatc
      481 tttggatcgg gcgggctgac tcaggcagag gagcagcaga ccagttcaca gttaccgtgg
      541 atggtgttgt ggtctttact gcagatgcaa ccgagatggg tgcttattca agctatacaa
      601 tggtcaatct ggatgtgagc acatttgccg acggcgcggc gcacgatatt ggtttttcag
      661 ccaacacatc cgggcaaatg gtcaatttca atctggatga tattacgctg gctgaagcca
      721 ttcccacatt ttctgatgtg ccgcttgatt attgggcgcg gtcttatatt gagcgcctgt
      781 acaactcagg gatcacacgt ggatgcggtt ccaacccatt gacctactgc ccgttggatt
      841 ctgtcacacg cacccagatg gccattttca ttttgcgcgg tattcatggc agcggctata
      901 ctcccccgga agccagcggg accgtatttt cagacgtcac aaccagcact tttggcgccg
      961 cctggatcga acaatttgca ttggaaggca tcacttccgg atgcggttct ggaaaattct
     1021 gtccaaatga ttatgtgacc cgcgcacaga tcgctatttt cctgctcaag gggaaacatg
     1081 gctcaacgta tgttccgcct gctgccacgg gtgatttcac cgatgtcccc gttggatcct
     1141 tcgcagccga ttggatcgag gaacttgccg ctgaaggtat caccagcgga tgcggcggcg
     1201 gaaatttctg cccaagtcaa aatgttaccc gcgaccagat ggcagttttc cttgtgaaga
     1261 ccttcaatct accgtagtaa acgaccttct tatcaaaggc gtctccatac ttttggaggc
     1321 gcctttttta ttttgaacat cgagttgttg tatttgtctt aaaaaccttg tatagtgata
     1381 aaagcgtttg tattgcccac aatttatttc ggaagggagc gtttcacatg tccgcgaaat
     1441 cacccgtttt ccgtatcctt ttggggatgc ttgttttttc cctcatattc tcagcagtgg
     1501 atgttgacgt tcgtgctgcg ccgttggctg atcctgttct agattggagt accttcctcg
     1561 ggggaaatgg cggcgattat gggcaggcga tggcagtgga tgttacgggg agtatttatg
     1621 tagttgggta cagcgattca gattggggga gtccgatccg tgcttttgat tccgcaaatg
     1681 atgcttttgt agcaaaattc gattcgtcgg gaaatctgct ttggaatact tttctaggcg
     1741 gcgggggaga cgatgttggt ataaacatcg ttacaagtgg gaacagcatt tttgtagcgg
     1801 ggtatagcag cagtgattgg gattgtgcgc cccttacctg caccatggat gactataccc
     1861 ttggttatga tgccttcgtg gccaagctcg actcttccgg taacttgaat tggatcacct
     1921 ttcttggggg aaatggcagt gactttgggc gtggaatcga tgtggatttc agcggaaata
     1981 tttatgtagt tgggggaagt gatggtacct ggggtagtcc gattcagtct catgctggcg
     2041 gggggtatga cggctttgct gcaaaattgg attcgaccgg tgcgcgaacc tggaatacgt
     2101 ttcaaggtag tgggggtgat gatcaaacct ggggcgttgc tttggatgtg agcggaaatg
     2161 cctaccttgc aggctatagc aacgatacct ggggcagccc agtgattgct tatgacgcag
     2221 actatgatgg ttttgctgcg aagctgaaca gcaacggtgc cttgatctgg aacaccttcc
     2281 tcggtggaaa cggagaagag cacagtcatg ccattgcctt ggatgggagc ggaacgattt
     2341 acgtaacagg gtatagcaca gacacttggg gcagtccggt acgggcttat acctctaatg
     2401 atgtatttgc cgcaaagctg acctcctccg gggctttgac atggaatacc ttccttgggg
     2461 gggcaggcgc tgatgctggg tatgcccttg ctttgagcgg gggagatatt tatgttacag
     2521 gttacagcac cgatacctgg gggagcccag atcgtgccta taccagcggg tttgatagtt
     2581 tcgctgcaaa actaaataca agcggtgtac tgacttggaa tactttcctt ggcggtagtg
     2641 gcagcgactt tagttacggt gttgcgcaga gtggcagtgg tttatttgtt ggtgggcata
     2701 gtgacgatac ttggggaacc ccgttacagg cttacgatac cgctgtggat tctttccttg
     2761 tgaaatttgc aggtgaaacg accccgccgc cagcctctcc gatctttgca gatgttccat
     2821 tcgatcactg ggctaatcca tgggttgaga cgttgtacaa tgcaggcgtt actggcggtt
     2881 gtgccacggc gccgcacttg atttattgtc ctgatgatgc tgtaacccgc gcccaaatgg
     2941 caatttttat cttgcgcagc atgcatggag gcgcctacct gcctccagca gcaacggggg
     3001 cagtctttgg cgatgtccct gctgatgctt ttgcggcaga ttggatcgag caattggcgg
     3061 ctgaaggggt gacgactggc tgtggcgacg gcaattattg tccggaggca gccgtgaccc
     3121 gtgctgagat ggcgatcttc ctgttgcgtg gaaagtatgg cggtgcttat actcctcctg
     3181 ctgcaacggg cacggtcttt ggcgatgttc cctccgattc ctttgcggca gattggatcg
     3241 aacaactcgc ctcggaaggt attactgtcg gctgcggaga tggtaattac tgcccggata
     3301 tgaatgtgac ccgtgctgag atggcggtct ttttggtaaa gacgttccat ttgccatagg
     3361 aagttacgtt ctccaatcca ttgtccgaaa caagagccgc agagtttcct gctgtggctc
     3421 ttgtttttgt gaagaaaacc ttgttgaaat ggatgtgacc atgaaaacca taaaatttct
     3481 atgggtgacg cttgtcgcct caatgttgtt gttcgccttg atgttgcctg gaggaaaagc
     3541 aaagagcaat ggcatgcagc aggcaccgat tggtgtaggt ggaactgcct ggaatctgat
     3601 ctggagtgat gaatttgatg gttcggcagt gaacagcaat gactggaatt tcaatgtggg
     3661 tggggaaggc tgggggaata acgaattgga gtattacacc gacggccaaa acgtgacggt
     3721 tgccaatggt gagttggtga tcacagctaa aaatgagaat ggcgggtatg cctgctggta
     3781 tggcccctgt gaatatacct ccactcgtat gaccaccaag gccaaacagg aattccaata
     3841 tggcagggtc gaagcgcgga tgaaattgcc gaatgggcag ggtttatggc ctgcattttg
     3901 gatgctggga agcaattttg atcaggtggg ctggcctgcc tcgggcgaag tggatgtgat
     3961 ggagcatatc aattctgaag atatcgtgta tggaaccatg cattgggatg ctggcggcta
     4021 tgcctcatac ggctgtgttt cgacggatgt gcttgccagt gtggatgtga cccaatacca
     4081 cgtctatgcc attgagtggg atacggaaaa gatcgtctgg tatgtggatg atacccaata
     4141 ctgtgaggcc aatattcgag acagcatcaa cagcacagag gaatttcacg cacccttcta
     4201 cattttgctg aatcttgcag taggcggaaa ttggcctgga gcacccgatg ccggcacggc
     4261 cttccctgcc agtatggcag tggattatgt ccgggtgtat tctgcagacg tgacctccat
     4321 ttttacagat gtgccgccca cttattgggc atggaatttc attgagcggt tgtataacgc
     4381 gggcattacc ggtggatgca gcacagcccc attgatgtat tgcccggaag ccacagttac
     4441 acgtgcgcaa atggccattt ttattttgcg gggtatgcat ggaagcgcct atgtaccgcc
     4501 agcggcgaca gggacggtct ttgcagatgt ccctctcggg gcgtttgctg ccgattggat
     4561 cgagcagttt gcggcggagg gggtgaccgc aggttgtggc ggcgggaatt actgtcccga
     4621 tgcaaccatc acccgcgcgc agatggccat cttccttttg cgcggtgaac acggcagtgc
     4681 ctacgttcct ccagttgcaa cgggtgcggt ctttggggat gtccctgctg gttcattcgc
     4741 tgcggattgg atcgaacagt tggctgcgga aggcatcacc agcgggtgtg gcggcggaaa
     4801 ctattgcccc gatagccccg tcacacgcgc tgagatggcg gtatttttgg tgagagcgtt
     4861 cagcctgcca tgatcaggtt gcattgatgc agtaatagga ctatttcggt cttgaattgg
     4921 ccttaaaaac cctgtatatt gatacaagat gcaattcagt gaaggtgttg tgaattgcac
     4981 agtgcaattt gaaagggatt atctgtatgt ctaccaaaac cgtcttccgt gctttttctg
     5041 ttttatttgt cttcgtcttt ttactggcac aagttggtgg ggcgggcgcg atttctccga
     5101 tggcgaacag cgttgtgttg acgcccggta gagcaattgc cagttggccg tctggtgttc
     5161 ctgccgggga gaaacggcct gttgtggtgt tcctgcccgg gtggggagga agcggagcgg
     5221 tcgatgcggc cgtatctgcc cagaatacga atcttgtgaa tcaggggtat gttactctgg
     5281 cgatcgggtt caattcgcct tcagattgga gcagtgatat tgatgtacaa accgccgatg
     5341 gtttggacaa actgtgtgcc gatgcttcca tccctgccaa ttgtgatgcc attattttgg
     5401 acggtgaatc ttatggcggt gcgcagaatt attatgtgat tgaatacatc cgcagcaatg
     5461 gatatggcgg aaaggctttg ggtttcgttt ctgaagatgc gggctatgct gcaccgggaa
     5521 acctgatcga tcccgtcacc ggcgagttcc agcggacggg tctggctgat actgcttcct
     5581 atggtgttgc catgatacag aatttgggcg atagcacctt ccctgtggat gattgtacat
     5641 ggggcaactg tggtgcccgt acattgagcg atgcacatca ggcgcagggc gataccaatg
     5701 tctttagcat ttgcccttca ggcggcgagc atggcacacg cggttatgct gactgggatg
     5761 catgggtggt ttctgccatc aagacccttt tccattccgt ttatggtgtg cctactttta
     5821 caggatatac cgctccaacg atctcggttg gcaatacttg tctgagctcg ggcgggtctt
     5881 ccacttttgt ggatgtgccg acgacgcacc cgttgtataa
//
LOCUS       DAOQLC010000192         5880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_5920_length_5880_cov_22.254592, whole genome shotgun sequence.
ACCESSION   DAOQLC010000192 DAOQLC010000000
VERSION     DAOQLC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5920_length_5880_cov_22.254592"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..900)
                     /locus_tag="PL000_19910"
     CDS             complement(4..900)
                     /locus_tag="PL000_19910"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91204.1"
                     /translation="MTKPTALIVEDDRDIVALFRHVLDIAGYQTEIMLNGKEAMSRLE
                     GFQPNIVLLDLQLPGMSGIEILSRMRADDRLRNVPVVVITAYAPYADSLPVEPDLLLM
                     KPVDIHQLSSLVQRLQATQGGLSESVHDRTTGLYTFPFFKVRLTFSLERIKQTEFRQF
                     GVLFADVAKINELKTKMPATEVNLFFRKLADHFKATLRPTDTMAWSLDNEFFLTLIED
                     IPTPEAPLRIAGRVRDSMKKFLETNDYGMNLRVSIGVLLCDYEYNDIEEILKDIDLAR
                     ARLREGLFTNPSVFDQSMLRGK"
     gene            1023..2357
                     /gene="yqeC"
                     /locus_tag="PL000_19915"
     CDS             1023..2357
                     /gene="yqeC"
                     /locus_tag="PL000_19915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HNC91205.1"
                     /translation="MDLAQALRLPLPASYHTVSLVGAGGKTTALFQLAQSLIQHGAPS
                     VILTNTTHLGDWQVAMAEHHVVAEDLPTLQDIPFSGITLVTGGFDHGRVMPVNESVII
                     WLHENCRAKNIPLLIEADGSRQKSLKAPAAHEPPIPEFSDTVIVVAGLSVLGKPLTDE
                     HVHRAEVFSTLGELPLGQPVDPEALGRVLAHPQGGLKNIPPHARRVVLLNQADTPALR
                     SIGGNLAPTLLEAFDSVIVSALQKNSFQTFEQTAGIILAAGKATRFGAPKQILDWKGK
                     PFVRQVAETALHSGLWPVVVVTGAFSADVESALSNLPVKIIHNPNYTQGQSTSIHAGI
                     EVISRQAGAAIFLLADQPQIPTEVIRALVETHTRDLSPIIAPLVLEEKRANPVLFDRV
                     TFPSLLNITGDVGGRAIFSDYKVEYLPWHDDRLLLDVDKPEDYQRLVEDETL"
     gene            2370..2948
                     /locus_tag="PL000_19920"
     CDS             2370..2948
                     /locus_tag="PL000_19920"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC91206.1"
                     /translation="MISAIVLAAGASKRMGEPKMLMPWGKSTVLQTIISTLQAASVND
                     ILVVTGGARQQVEMLVGKTVQTVFNSEYEKGEMLSSIQLGLSVKMHEASAALICLGDQ
                     PQVKERTVRSVCEAFLHNKARIVVPSYEMQRGHPWLIARPLWDEFLALKPPRTPRDFL
                     KKHAGKIQYVTVDTPSIIEDLDTPEDYLKYKP"
     gene            3026..3946
                     /locus_tag="PL000_19925"
     CDS             3026..3946
                     /locus_tag="PL000_19925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HNC91207.1"
                     /translation="MKYFIIVNPVSGRGLGERSISQIESSLRENGLDFTLVRTERMWH
                     AADLAEGAARDGYDVIVCASGDGTINEAINGIMKARKDGHTQAAFSALSIGTGNDFSG
                     GTGIPTNLNDSLKSLIANKRKKIDIGIVKGGDYPEGRYFGNGIGVGFDAAVGNEAIKV
                     RWTRGLPAYLIGVIKTVFLYYNPAQVEIVLDNQETIKQVSLMISVMNGRRMGGGFQMA
                     PESQPDDGFFDLCIAETASKGRILMLIPHFIRGTQMTQPEVKMRRAKKISIKSLDRTF
                     PAHADGEFICLSGSELTLELLPRELEIVCA"
     gene            3958..4638
                     /locus_tag="PL000_19930"
     CDS             3958..4638
                     /locus_tag="PL000_19930"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC91208.1"
                     /translation="MKFSLWLITFILRIYFRLTLRLDAQGMDTVPMQGPMIIISNHTG
                     QIEVPVLATLLQPRKITGWGKAEAFENPFLRWVFGAWGIIPVHRGEADMTALKSALRS
                     LKDGLIFGIAPEGTRNYSGKLRRALPGAVTLALHSGAPILPAAHWGGENFMKNFKQLK
                     RTDFHLRMGEPFVIDTKGAKVTAEMRQQIVDEMMVQLALLLPEEYRGEYTEMCKHETQ
                     FIKKQNAG"
     gene            complement(4658..5086)
                     /locus_tag="PL000_19935"
     CDS             complement(4658..5086)
                     /locus_tag="PL000_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91209.1"
                     /translation="MRIDIVTKADEELYEAFQRLVPQLTNNNPPPSLNDLADLVRDSS
                     STLMVARNEKQEIVGALTLAVYRVPTGIRSIIEDVIVDTSARGQGIGEALMQRAIEIA
                     KEKGAGNISLTSNPLRESANRLYLRVGFKKRETNAYQMKF"
     gene            complement(5076..5804)
                     /locus_tag="PL000_19940"
     CDS             complement(5076..5804)
                     /locus_tag="PL000_19940"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNC91210.1"
                     /translation="MTTSAISENKPNPLRVIAAWGVHLFTATGAIWGFLAMLAIFEHD
                     WRSMIIYMVIAMLVDGFDGMLARWADVKTYANGIDGALLDNILDYLNYVVVPALFLME
                     AGVLPYGLQMIGAFSILITSAYQFTQTDAKTDDHHFKGFPSYWNVMALYMLLMNLPVW
                     VNFGFLVAFNILVFVPIKYIYPSRNSYLRTLTLALTYLYGAIGIWGLLLYPNTPKWVV
                     WASFIYVGYYLVLSLIPKNKSNAN"
BASE COUNT         1481 a         1562 c         1534 g         1303 t
ORIGIN      
        1 gctttattta ccgcgcagca tggattgatc gaacaccgaa gggttggtaa acaaaccttc
       61 acgcagacgg gcgcgggcaa ggtctatgtc tttcaaaatc tcttcgatgt cgttgtattc
      121 ataatcacac agcagcacac cgatgctcac gcgtaaattc atgccgtagt cattggtttc
      181 gagaaatttc ttcatagagt cacggacgcg ccccgcgata cgcaacggcg cttcgggggt
      241 ggggatatct tcgatcaagg tcaggaaaaa ctcattatcc agcgaccagg ccatggtgtc
      301 ggttgggcga agcgtggctt tgaaatgatc cgccagtttg cgaaagaaaa gattcacttc
      361 cgtggcgggc atctttgttt tcagttcgtt gatcttcgcc acatctgcaa acaaaacgcc
      421 gaattgtcga aactcggtct gtttgatcct ctcgagtgaa aaggtcaagc ggaccttgaa
      481 aaacgggaag gtgtaaagcc ccgtcgtcct gtcatgcact gattcgctca aaccgccctg
      541 cgtagcctgc aaacgctgaa ccaggctgct cagttggtga atgtccactg gcttcatcaa
      601 aaggagatca ggttccacgg gcaggctgtc ggcatacggc gcatacgctg tgatgaccac
      661 aacaggcaca ttcctcaacc tgtcatcggc gcgcatacgt gacaagatct caataccaga
      721 catgcccggt aattgcagat ccagcaaaac gatattcggt tgaaaccctt caaggcggct
      781 cattgcctct ttgccgttga gcatgatctc ggtttgatat cctgcaatat ctagcacatg
      841 ccgaaacagg gctacaatat cgcggtcgtc ctcaacgatg agcgcagttg gtttggtcaa
      901 agcctgctac ctttccgata aaaaaacaaa aaaatcacat aaagataact cattatacaa
      961 ttgaagatgg ggaaaacgga ataacaaaat ggattgcaat acttctgatt tacaattgaa
     1021 gcatggatct tgcacaagcc ctgcgcctgc ctttgcccgc atcatatcac accgtaagcc
     1081 tggtcggtgc gggcggtaaa accaccgccc tgttccaact ggctcaaagc ctgattcagc
     1141 atggggctcc atcggtcatt ctgacgaaca ccacacacct gggagattgg caggtggcaa
     1201 tggccgagca tcacgtcgtt gcggaggacc tgcccacgct acaagacatt ccattctctg
     1261 gcattaccct tgtaacaggc ggttttgatc atggcagagt catgccagtg aacgaaagtg
     1321 taattatttg gctacacgaa aattgccgag caaaaaatat tcccctgctt atcgaggcag
     1381 atgggtcccg ccaaaaatcc cttaaagcgc ctgccgccca cgaaccgccc atcccagaat
     1441 tttcggacac agtcattgtc gttgcagggc tgagcgtgct tggaaaacca ctcaccgatg
     1501 aacatgtcca ccgtgctgag gtcttttcaa ctctgggtga attgccactt gggcaacccg
     1561 ttgaccctga ggcacttggc cgtgtcctcg cccacccaca aggcgggctg aaaaacattc
     1621 cgccccatgc acggcgtgtg gttctgctca atcaggccga cacacccgcg ctgcgatcca
     1681 tcggcggcaa tctggcgccc accctgcttg aggcgtttga ttccgtcatc gtcagtgctt
     1741 tgcagaaaaa cagttttcaa acattcgagc aaaccgcagg catcatcctc gccgctggaa
     1801 aagcgacccg ctttggtgcg ccaaaacaaa tattggattg gaagggcaaa ccgttcgtgc
     1861 gacaggtggc ggaaactgcg cttcattctg gactttggcc tgtggtggtc gtcactggcg
     1921 ctttctccgc tgacgttgaa tctgccttaa gcaaccttcc cgtaaagatc atccacaacc
     1981 ccaactacac ccagggacaa agcacctcca ttcatgcagg cattgaagtc atcagcaggc
     2041 aggctggagc agccatcttc ctcctcgctg accaaccgca gattcccaca gaagtgattc
     2101 gcgccctggt cgaaacacac acacgagatt tatctcccat cattgcgccg ctggtattgg
     2161 aagaaaaacg cgccaacccc gtgttattcg accgagtcac atttcccagc ttgctcaaca
     2221 tcacaggtga tgtaggcgga cgggcgattt tctcagatta caaagtagaa tacctgccat
     2281 ggcatgatga caggttattg cttgatgtgg ataaacctga agactatcaa agattggttg
     2341 aagatgaaac tttgtaaaag aggcaaaaaa tgatcagcgc cattgtactg gctgcaggcg
     2401 catcgaaacg catgggcgaa cccaagatgc tgatgccctg gggaaaaagc acggtactac
     2461 aaaccatcat ctccactttg caggcggcaa gcgtgaacga tattctcgtt gtcacaggcg
     2521 gggccaggca acaggttgaa atgctggtcg ggaaaaccgt ccaaaccgta ttcaattcgg
     2581 aatatgaaaa gggcgaaatg ctcagctcga tccaactggg gttgtcggtg aaaatgcatg
     2641 aggcgagtgc agcgctcatt tgtctcggtg accagcccca ggtcaaggaa agaaccgtgc
     2701 gcagcgtctg cgaagcattc ctccacaaca aggccaggat cgtggtgccg agttacgaaa
     2761 tgcagcgcgg acatccctgg ctgatagccc gaccattatg ggatgaattc cttgcactga
     2821 agccgccacg aaccccgcgt gattttttga aaaagcacgc aggcaagatc caatatgtaa
     2881 ccgtggatac gcccagcatc atcgaagacc tcgacacccc cgaagactat ttgaaatata
     2941 aaccgtgagc ggtgcgggca gtgcagattt gacaatccgc cttacaaaag atacactcac
     3001 ttcaatttcc aattgggagc gcagcgtgaa atatttcatc attgttaatc cagtatcggg
     3061 gcggggcctc ggcgaaagat ccatttccca gatcgaatcc agcttgcgtg aaaacgggct
     3121 ggattttacg ttggtgcgca ccgaacgcat gtggcacgcc gccgatcttg ctgaaggtgc
     3181 cgcacgtgac ggatacgatg tgatcgtatg cgccagcggc gatggcacca ttaatgaagc
     3241 catcaacggc atcatgaaag cccgcaaaga cggtcacacg caggcggcgt tttcagccct
     3301 gagcattggc acgggcaacg atttttcggg cggcacgggc atccccacca acctcaacga
     3361 cagcctgaaa tcgctgatcg caaacaaacg caaaaaaatt gacatcggca ttgtcaaagg
     3421 cggcgattat cccgaaggcc gttatttcgg caacggcatc ggcgttggtt tcgatgcggc
     3481 tgtcggcaac gaagccatca aagtccgttg gacgcgcgga ctgcctgcct acctcattgg
     3541 cgtgatcaaa accgttttcc tttattacaa tcccgcgcag gtcgagatcg ttctcgataa
     3601 tcaggaaacc atcaaacagg tttcgctgat gatctctgtg atgaacggtc ggcgcatggg
     3661 cggcggtttt caaatggccc ccgaaagcca gcccgatgac ggtttcttcg atctgtgcat
     3721 cgccgaaacc gcttccaaag ggcgcattct catgctcatt ccccacttca tccgcgggac
     3781 tcagatgacc caacccgaag taaaaatgcg acgcgccaaa aagatctcga tcaaatcact
     3841 ggatcggact ttccccgcac atgcggacgg ggaatttatc tgcctcagcg gttcagaatt
     3901 gacccttgaa ctgctgccgc gtgaattgga aatcgtctgc gcctgagcat tttccccatg
     3961 aaattttccc tctggcttat cactttcatc ctgcggatct atttccgcct caccctgcgc
     4021 ctcgacgcac aaggcatgga caccgtcccc atgcagggac cgatgatcat catctcgaac
     4081 catacggggc agatcgaagt gcccgtcctt gccaccttgt tgcagccgcg caaaatcaca
     4141 ggctggggca aggccgaggc gtttgagaat ccatttttgc gctgggtctt cggcgcgtgg
     4201 ggcatcatcc ccgtccatcg cggtgaagcg gatatgaccg cactcaagtc ggcgctgcgc
     4261 tctttgaagg atggtctcat ttttggcatt gcccccgaag gcacccgcaa ttattcgggc
     4321 aaattacgcc gcgccctgcc tggtgcagtg actttggctc tgcattctgg cgcgccgatc
     4381 ctccccgcgg cacattgggg cggagagaat tttatgaaaa acttcaagca gttgaagcgg
     4441 acagattttc atttacggat gggagagccg ttcgtgatcg acacaaaggg cgccaaggtg
     4501 acggctgaaa tgcgccaaca gatcgtggac gaaatgatgg tgcagcttgc gcttttgctg
     4561 cccgaagaat accgcggcga atacacagag atgtgcaagc acgaaacaca atttatcaaa
     4621 aaacaaaacg cgggctgaaa agtccgcgtt ttgtttttta gaatttcatt tgataggcgt
     4681 tggtctcgcg ctttttgaat cccacccgca aatacaatcg attcgccgac tcgcgcagtg
     4741 gatttgacgt gagcgagatg ttgcccgcgc ctttttcttt ggcgatctcg atggcacgct
     4801 gcatcagcgc ttcgccgatg ccttgtccac gggcggaggt gtcaacgatg acatcttcga
     4861 tgatggagcg gatgcctgtc ggcacacggt aaacggccaa cgtgagcgcg ccgacgatct
     4921 cctgcttttc gttgcgagcc accatcagcg tggacgatga atctcggacg aggtcagcca
     4981 gatcgttaag agaaggaggc ggattgttat tcgtcaactg cggcacgagg cgttgaaacg
     5041 cttcgtagag ttcctcatcc gcctttgtaa cgatgtcaat tcgcattgga tttgtttttg
     5101 gggatcaggc tcaacaccag ataatatccg acataaatga acgaagccca gaccacccac
     5161 ttgggggtgt tggggtaaag cagcaagccc cagatgccaa tggcgccgta gagataagta
     5221 agggcgagcg taagggtccg caggtaactg ttgcggctgg gataaatata tttgatggga
     5281 acaaagacaa ggatattgaa cgcgaccaaa aagccaaagt tcacccacac tggcaggttc
     5341 atcaacagca tgtacaacgc catcacgttc caataagagg gaaagccctt gaagtgatgg
     5401 tcgtcggtct tggcatcggt ttgggtgaac tgatacgccg aggtgatgag aatggaaaat
     5461 gcgccgatca tttgcaagcc gtagggcagc acacccgctt ccatgagaaa gagcgcgggc
     5521 acaaccacgt aattcaggta atcgagaatg ttatcaagca aggcgccatc aatgccgttg
     5581 gcgtatgtct tgacatcagc ccagcgtgcc aacatgccgt caaaaccatc caccagcatc
     5641 gcgatgacca tgtagatgat catggagcgc cagtcatgct cgaaaatggc aagcatggcc
     5701 agaaagcccc agatcgcgcc tgtggctgtg aaaagatgca ctccccatgc ggcgatgacg
     5761 cggagagggt tgggtttgtt ttctgaaatt gcggaggttg tcatgggttc tgtccttggt
     5821 tggggtgaaa ggtgctggaa ttatactcat gtctggggga agttgagggg tgcaggttat
//
LOCUS       DAOQLC010000193         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6047_length_5797_cov_20.243817, whole genome shotgun sequence.
ACCESSION   DAOQLC010000193 DAOQLC010000000
VERSION     DAOQLC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_5797_cov_20.243817"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4580
                     /locus_tag="PL000_19945"
     CDS             <1..4580
                     /locus_tag="PL000_19945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC91211.1"
                     /translation="RSIFNGDVVDVLLFNSDTRMITLAYSYFDNQYYSDEPPWELGEE
                     GLTSKVISTCQPLLLHTAEEMAQHGAAAYLNSPKQGEDPESYLGVPILIGDKVLGVVD
                     VQSYAKHAFNLDNLHLLQTLSSNIGVALENARLFEETRRLLNVTEERAAELSVINRIQ
                     QGLAAELDPQAIIELTGDKLREVLHTEDLGIRLYDTQNNLIHYVYEYIQGERRNYPSK
                     PPVKGGTFERISKTRQPFVRNTLAEREALGLNLLPGTEAAKSSVNVPIIGSDRVIGAI
                     IVQSHEREYAFSEAQVRLLQTVAASMGVSLENARLFDETQRLLKVTEDRAAELAIINS
                     VQEGLASKLDMHAIYDLIGDKLGEILHTHDIDIRIFDPANNKVYFPYLKEKGTRIEIQ
                     SSEFRGMSKYVYDTKQMLVVNEDLPAFMKKVGSNTLPGTQMEKSFVALPITSNGEVIG
                     MVGISDYEKENAFSESELRLLQTVVSSMSVALENARLFDETQRLLKETEERNAELAVI
                     NSVQAALSTEISVEGIYDAVGSKMREIFKAEIGIRIYDPVTNLIHLPYLYEKGERVHP
                     EPYPLTDKGFAAHVFRTRETLVVNENAAEVREKFGSYLLPGTASAKSSIYVPMIISGQ
                     VRGLVSISNYEREHAFSDSIVRLLQTLVNSMGVALENARLFGETQRLLRETEQRAAEL
                     GAIGAVTQALVAETELDKLIQLIGSQTRDIFNADIAYLALYDSKAGNIHFPYQHGDTF
                     PSIQLGEGMTSRIIQNGHPLLFNRNLDEQSVAMGINRMGRKARSYLGVPIKAGRETIG
                     VISVQSTQKEGAFNEDSLRLLTTIAANAGSAIHTAQLHAETERRAREMATLTEVGRDI
                     SSTLDSSTVLERISQNAMELLNGELSALFLPENNGLIFRAIAAVGQNADELRNDTIEL
                     GAGILGAIAMNKVGEIVNDANNDPRALTIAGTENTPDEHMLAVPMLANDELKGLMAVW
                     RTGAERQFTEAELEFLNNLSRQAVIAVQNSQLFAEAQEARAAAENANQAKSAFLATMS
                     HELRTPLNAIIGFSRIVRKKAEGVLPEKQTENLDKVLSSAEHLLGLINTVLDIAKIEA
                     GRMDVQAANFNINALVDQCYNTAQPLVKANVEFKKSNDTNLPLIFSDQDKIKQIILNL
                     LSNAAKFTHKGSVTISTRYAESNFMVDVTDTGIGMSEEALGRIFEEFQQADSSTTRQY
                     GGTGLGLAISRNLARLLGGDLTVTSEQGKGSVFTLSLPIQYQSEVSPASSSDPQTGST
                     QKTEPRLKVTSTKKRVLVIDDDPDAVYLLQETLNTNEFEVVGARSGMDGHRLARQLMP
                     DAILLDILIPDKDGWQVLHDLKVDERTANIPVILLTIVDKKALGFRLGASAYLLKPLN
                     PREVIDTLSRVTKKIGRTHIHVLVVDDDPHIPDMLKQILPASEFDLRSAEDGVQGLEA
                     IAMSVPDVLLLDIMMPRLDGFGVIKRLRANPATRELPIIVISAKELTDDEAARLKESV
                     AFIMRKQGFDGEKLVEEIRSVAHLPPGVKSS"
     gene            4621..>5797
                     /locus_tag="PL000_19950"
     CDS             4621..>5797
                     /locus_tag="PL000_19950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNC91212.1"
                     /translation="MNNQPKILIVDDEPFNVDYLEQELEDLNYTTVTAVNGQEALEKI
                     SSEAPDLVLLDIMMPIMDGFSVLGKVKADPAIRDIPIIVISANNDLQSTVKGIQLGAE
                     DYLPKPFEPTLLKARIQSSLEKKRLRDMQSLYLKSLEREMNIARDIQKEFLPAQLPEV
                     PGWEIASYFEAAKEVAGDFYDAFTLPDGTLTFLVADVCGKGIGAALFMTLFRSLIHAA
                     SISDQFSVGQAQPSLSAAERLQHAVTLTNNYVAETHEASNMFATVFVGILDPATGKLT
                     YINAGNEPPLIAGGDGKVHTTLARTGSAVGAIANAKFTVKETTLASDDMLVAFTDGIP
                     DTQNIHGEFFGNDRLRGLLVDNSTPAALLKAMETDLDQFIGEADQFDDITLLAIKRKA
                     "
BASE COUNT         1485 a         1798 c         1420 g         1094 t
ORIGIN      
        1 tccgcagcat cttcaatggc gatgtggtgg atgtactttt attcaactcc gacacaagga
       61 tgatcaccct tgcctacagc tatttcgaca accaatacta ttccgatgaa cccccgtggg
      121 aattgggaga agagggtctt acctcaaagg tcatctcgac ctgccagccc ctgctcctgc
      181 acactgccga agaaatggct caacatggcg cagcagccta tcttaactcg cccaaacaag
      241 gcgaggatcc cgaatcctat cttggggtgc cgatcctcat cggtgacaag gtgctcggcg
      301 tggtggatgt acaaagctat gccaaacacg cattcaacct ggataacctc cacctgcttc
      361 aaacactttc ttccaacatc ggggtggcac tcgaaaacgc gcgcctcttc gaggaaaccc
      421 gccgcctgct caatgtaacc gaggaacgcg ccgctgaact gtccgtcatc aacagaatcc
      481 agcaagggct ggcagcggaa ttggatccgc aagccattat agagttgact ggcgacaaac
      541 tgcgtgaagt gcttcacacc gaagacctgg gcattcgctt gtacgacacc caaaacaacc
      601 tcatccacta tgtgtatgaa tacatccagg gtgaacgccg caactaccca tccaaaccac
      661 ctgtaaaagg cggcaccttc gagagaatct caaagacccg tcaacccttt gtgcgcaaca
      721 cactcgccga acgcgaagcg ctggggttga atctcctgcc aggcaccgaa gccgccaaat
      781 ccagcgtcaa cgtacccatc atcggcagcg accgtgtgat cggtgccatc atcgtacaaa
      841 gtcacgaaag ggaatacgcc ttcagcgagg cgcaagtgcg cctgttgcaa actgtggcgg
      901 caagcatggg tgtgtcgctc gaaaatgcgc gcctcttcga tgagacccag cgcctgctca
      961 aggtgacaga agaccgtgcc gctgaactcg ccatcatcaa ctcggtgcag gaagggctcg
     1021 cctccaagct ggatatgcac gccatctacg acctcatcgg cgacaaactg ggcgagatcc
     1081 tgcacacaca cgatattgac atccgtattt ttgacccagc caataacaaa gtgtacttcc
     1141 cctatctgaa ggaaaaagga acgcggatcg agatccaatc gtcagaattc aggggaatgt
     1201 cgaaatatgt gtacgacaca aagcagatgc tggtggtcaa tgaagacctg cctgctttca
     1261 tgaaaaaggt cggttcaaac accctgcctg gcacacaaat ggaaaaaagt tttgtagccc
     1321 tgcccatcac cagcaatgga gaagtgatcg gcatggtagg catcagcgat tacgaaaagg
     1381 aaaacgcctt cagcgaatca gagctgcgcc tattgcagac ggtggtttct tccatgagcg
     1441 ttgccctcga aaacgcccgc cttttcgacg agacccagcg cctgctcaaa gagaccgagg
     1501 aacgcaacgc tgaattggcg gtcattaaca gtgtgcaggc ggccctctcc accgagatca
     1561 gcgtggaagg catctatgat gcggtcggaa gcaagatgcg cgagatcttc aaagccgaga
     1621 tcggaatccg tatctacgac cctgtcacaa acctgatcca cctgccctac ttgtatgaaa
     1681 agggtgaacg cgtccacccc gaaccctatc cgctcaccga caagggattt gccgcccatg
     1741 ttttccgtac ccgcgaaacc ctggtggtga acgaaaacgc ggcagaggta cgcgaaaaat
     1801 tcggttctta tcttctgcca ggtacagcct cagccaagtc atccatttat gtgccgatga
     1861 tcatcagcgg acaggtccgc gggctggttt ccatcagcaa ctacgagcgc gaacacgcct
     1921 ttagcgactc gatcgttcga ctcctgcaaa cattggtcaa ttccatgggc gtggcgcttg
     1981 aaaacgcccg tctcttcggc gagacccagc gcctgctgcg tgaaaccgaa caacgcgccg
     2041 ccgaattggg cgccatcggt gcagtcactc aagccctcgt ggcagagact gaactcgata
     2101 aactgatcca gcttatcggg agtcagaccc gcgatatttt caatgcagat attgcctatc
     2161 tggccttgta cgattccaag gcaggcaaca tccacttccc gtatcagcat ggcgatacct
     2221 tcccatcgat tcaactcggc gaaggcatga ccagccgcat catccaaaat ggacatcccc
     2281 tgctcttcaa ccgcaacctg gatgagcaaa gtgtggcgat gggcatcaac cgcatgggca
     2341 gaaaggccag gtcgtatctg ggcgtgccca tcaaggctgg ccgcgaaacc atcggcgtga
     2401 tcagtgtgca aagcacacaa aaggaaggcg ccttcaacga agattcgctg cggctgctca
     2461 ccaccattgc cgccaatgca ggctcggcca ttcataccgc gcaactgcat gctgaaacgg
     2521 aacgccgcgc ccgcgaaatg gccaccctca ccgaagtggg ccgcgatatt tcctcaaccc
     2581 ttgattcctc caccgtgctg gagcgtatct cccaaaatgc aatggagctg ctcaacggtg
     2641 aactcagcgc gctcttcctt cccgaaaaca acggcctgat cttccgtgcc attgcggctg
     2701 tggggcaaaa cgccgacgag ctccgcaacg acaccatcga actcggagca ggcatccttg
     2761 gcgccatcgc catgaataag gtcggcgaaa tcgtcaacga cgccaataac gacccgcgtg
     2821 cgctcaccat cgcaggcact gaaaacacgc ccgacgaaca tatgctggca gttcccatgc
     2881 tcgccaatga tgaattgaaa ggcctcatgg cggtctggcg cacaggcgca gaacgtcaat
     2941 ttacagaagc cgagcttgaa ttcctcaaca acctttcacg tcaggccgtg atcgcggtgc
     3001 aaaattcaca actctttgcc gaagcgcagg aagcccgtgc agcggctgaa aatgccaatc
     3061 aggccaagag cgccttcctt gccaccatga gccacgagct acgcaccccg ctcaacgcca
     3121 tcatcggttt cagccgcatc gtccgcaaga aggcggaagg tgtgctgccc gaaaagcaga
     3181 ccgagaatct cgacaaagtc ctttccagcg ccgaacacct gctcggcctc atcaacaccg
     3241 tgctcgatat tgccaagatc gaagcaggcc gcatggatgt gcaggccgcc aacttcaaca
     3301 tcaacgcgct ggtcgatcaa tgttacaaca ccgcccaacc actggtcaaa gcaaacgtcg
     3361 aattcaaaaa gagcaacgac accaatttgc cgctcatctt ctccgatcag gacaagatca
     3421 agcagatcat cctcaacctg ttgagcaacg ccgccaaatt cacacacaaa ggcagtgtca
     3481 ccatcagcac gcggtatgct gaatcaaatt tcatggtgga tgtgaccgac acaggcatcg
     3541 gcatgagtga agaagcactt ggccgcatct tcgaagaatt tcaacaagcc gattccagca
     3601 ccacccgcca atacggcggc acagggcttg gccttgccat cagccgaaat ctggcgcgcc
     3661 tgctcggcgg cgacctgacc gtgaccagtg aacagggcaa gggttctgtc ttcaccttga
     3721 gcctgcccat acaatatcaa agcgaagttt cacccgcttc ttcatcggac ccgcaaactg
     3781 gttccacaca aaagacagaa ccgcggctga aagtgacctc taccaaaaaa cgcgtcctgg
     3841 tcattgatga tgaccccgat gccgtgtacc tgttgcagga aaccctcaac acgaacgaat
     3901 tcgaagtcgt cggtgcccgc agcggcatgg acggtcaccg cctggcgcgt caactcatgc
     3961 ccgatgccat cctgctcgac attctcattc cagacaagga cggctggcag gtgctgcacg
     4021 acctgaaagt ggatgaacga accgcaaaca tccccgtgat ccttttgacc attgtggaca
     4081 aaaaagcgct cggattccgc ctcggcgcct cggcctacct gctcaaaccc ctaaacccgc
     4141 gcgaagtgat cgacaccctg agccgtgtca ccaaaaagat cgggcgcaca cacatccacg
     4201 tgctggttgt ggatgatgac ccgcacatcc cagacatgct caaacaaatt ctgccagcct
     4261 ccgaattcga cctgcgctct gcagaagatg gcgtacaggg actggaagcc attgccatgt
     4321 cggtgcctga tgtactcctg ctggatatca tgatgccgcg cctcgatggg ttcggggtca
     4381 tcaaacgcct gcgcgccaac cccgccacac gcgaactgcc cattatcgtc atcagcgcca
     4441 aggaactgac cgatgacgaa gccgcccgct tgaaggaaag cgtcgcattc atcatgcgca
     4501 agcagggttt tgacggtgaa aagctggtgg aagagatcag aagcgttgcc catcttccgc
     4561 ctggagtaaa atcgtcctga tctttatcga agccgcattg atttcaacct acggagccgc
     4621 atgaacaacc aacctaaaat tctgatcgtg gatgacgaac ccttcaacgt ggactatctc
     4681 gaacaggaac tcgaagacct gaattacacc accgtcaccg cggtcaacgg gcaggaagcc
     4741 ctcgaaaaga tcagcagcga agcccccgac ctggttttgc tcgatatcat gatgcccatc
     4801 atggatggtt tcagcgtgct tggaaaggtc aaagccgacc ccgccattcg cgatatcccc
     4861 atcatcgtca tttcagccaa caacgacctg caaagcaccg tgaagggaat ccaactcggc
     4921 gcggaagact atctccccaa accgttcgag ccgaccctgt tgaaggcacg catccaatcg
     4981 agccttgaaa agaagcgcct tcgcgatatg caaagcctct acctcaagag cctcgaacgt
     5041 gaaatgaaca tcgcacgcga cattcaaaaa gaattcctgc ctgcgcaatt gcccgaggtg
     5101 ccaggctggg agatcgcttc gtatttcgaa gccgccaagg aagtggctgg cgatttctac
     5161 gatgccttca ccctgcccga tggcaccctg accttcctcg tagccgatgt atgcggaaaa
     5221 ggaatcggcg cggcattgtt catgaccctc ttccgcagcc tcatccacgc ggcttccatt
     5281 tccgaccaat tctctgtggg gcaggcacag ccatcccttt ctgcggctga gcggttacag
     5341 cacgccgtca cgttgaccaa caactacgtg gccgaaaccc acgaagcatc caacatgttc
     5401 gccacggttt tcgtcggcat cctcgacccc gccaccggaa agttgaccta catcaacgcc
     5461 ggcaatgaac cgccgctcat cgcaggcgga gacggcaagg tccacaccac cctcgcgcgg
     5521 acaggttccg ccgtcggcgc gatcgcaaac gccaaattca ccgtcaaaga aaccacctta
     5581 gccagcgacg acatgcttgt cgctttcacc gacggcatcc ccgatacaca aaacatccac
     5641 ggcgaattct ttggcaacga ccgtctgcgc ggcctgcttg tggacaactc cacacccgcc
     5701 gccctgctga aggccatgga aaccgacctc gatcagttca tcggcgaagc agatcaattc
     5761 gacgacatca ccttgctggc gatcaaaaga aaagcct
//
LOCUS       DAOQLC010000194         5780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6075_length_5780_cov_21.991092, whole genome shotgun sequence.
ACCESSION   DAOQLC010000194 DAOQLC010000000
VERSION     DAOQLC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6075_length_5780_cov_21.991092"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..5053
                     /locus_tag="PL000_19955"
     CDS             470..5053
                     /locus_tag="PL000_19955"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC91213.1"
                     /translation="MSIAGIRSNRGDGYQTLVAFDWALTVLSDENYQWLEIDSILYPV
                     DDVVVGKSDGTVIACQCKKNHTDFEAWSINDLASELQKAVRLLSQHRNIEVRFYSRNN
                     FGPIGKLKEYCSTQRDALSYKTDLGAMNRSTDNNLSNLISAVAPGFSNYEFLSRTEFE
                     TSNTLDRMASQLRERLRNIASNPDAAFNALWVHLDQLGARMSVGDSSAGAQHRLIKHD
                     LEAILNKAGALLAPPLNLDDIRASFSNTSAIGRSWRRDVAGKRIVTPVINELLAAINA
                     KKRAILLTGLPGSGKTCAMLELQEALEQRAQLHSDIVPLFIQSREFADLATSHDRQTL
                     GLSEQWVNQAARMAERAWVVVVLDSLDVLSIARDHRILTYFLAQIDRLLLVPQVTVVA
                     ACRDFDKQYDRRIAEREWDSEIKCLPLDWNTDVAPLLDKLAISSADIDAATRELIRNP
                     RELALFVELALRGGGFSVVTSQALAQRYLDVIVRADTSLGDTAIQAIEAVAQRMLESR
                     RLTVPHQQFQSPQEILRSLFSLNILQDTQDGKLTFGHQTLLDVLVISRALRKGVTLNK
                     FIQSLPPVPFVRPCIRSFVAQLAVGERREFRKQLRAVLTGNSAFHIRRLVAESLSEQP
                     PEDDDWPLMRELREKHRDVFQAIYYSAKTIEWHYFWLKHLVPVLKAAQDAEGYASHAY
                     RMTQWKNDDTQGVLLYWQEALSLEWIDASRIAEQLPYFISEINSDKLAFVVPLLQQLL
                     DMPQPDHSVLGQSIARCVEAGAVGDNVLWRYAMNGVDEKSIAEYNLHNRLRFRAHEFG
                     DRKDNFFGQRMLKSIALLDLAIESIERWSNACTLCYGETRIGYRHGFLNETSYRIEHS
                     QQDVHHAGSMNVLLSAVEASILHHAMEHSEWWTNNRERLCFNHEGALLYFAVQACTAS
                     PEANIDLIGRMLSDRNMLEFGLQYELGSLIRSAFTSLNSTTQDAAMATILSIWDEPTT
                     EDSEKSWILKKRTELLKPIPSHLRSAEAQATIDAFERTNGAFVRQPDIFMRGGTVHAP
                     FSFEIFLSARDNQILRLIAHYRGHSDWHGTGADFLIGGEREVGWQLREASSRHPTRFL
                     QLLPTYWAYISEQFRDDIMDGIATYLAYRHGQLRRDDNWLPLEEPDASTLAGHILDEL
                     ERHPLYWQMRRTAANALKVCANIVRSPDDAKRLVFLARGFDLLDKNDPIKGDNIDLIN
                     LGIDMAKGNIAEALLTLANNLSENNLDLPSLLEPALARCATEEHPAIRAVMLLHLPSL
                     QDKNFELSWKLFYLIMQDARGLWQLAERCLYYAYHNRFETVRPVLARLRIEGRDKDLE
                     TWGRISALAGMARHIDFFELLIELKALESTDAWRGAASVWTNSENIQHHRNECFAGIE
                     AGLNAGESCALNVAEQIDNIFRDEATPIPLPIEIIRGCFSAFENDNNTDNRHHRLLGL
                     HEWLNAVSQNDPDLSLAVTKLYVNYASRCGPHIYDHKNSLTQLMTRLFAEAEEREESD
                     EGAMLRSVVEIQDALLSLGVNGVADWLKAAERP"
     gene            5315..5399
                     /locus_tag="PL000_19960"
     tRNA            5315..5399
                     /locus_tag="PL000_19960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Leu,seq:cag)
     gene            5454..5530
                     /locus_tag="PL000_19965"
     tRNA            5454..5530
                     /locus_tag="PL000_19965"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5488..5490,aa:Asp,seq:gtc)
     gene            5543..5618
                     /locus_tag="PL000_19970"
     tRNA            5543..5618
                     /locus_tag="PL000_19970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5576..5578,aa:Gly,seq:gcc)
BASE COUNT         1444 a         1583 c         1461 g         1292 t
ORIGIN      
        1 atttccacac cctcatcctc ggcgccaagg accaggccaa agccatgtac ggccccgatt
       61 ccgatcacgt cgccgcgttg gggctcaaga agaagtcgga taagaaggcg cgcgggcgga
      121 cggcgaaggc gaaaccgacg gagtaagatg atgcggaggt aatggaaggg ccgtcccgtt
      181 ggcgtgatga agggctgacg gggcggcctc tttacttaga agggaattat tcatcttgag
      241 catcaaacag cttcgggcag ttgttgaaat tcgagccccc gctaatgtaa agcttctagt
      301 catccaaagt cgcctttgat agagcgcact gaatcaaact gcaacaactg cgcgaccact
      361 actccgacta tgaactggca aacggcgcct gaatgactca tagtgatcgt cacgaattga
      421 agaccgtgta aatcagggaa tggaaatctc tgacagagga aaccgaaaga tgagtattgc
      481 aggcatacgc tcaaaccgtg gcgacggata ccaaacccta gtagcatttg attgggcgct
      541 gaccgttctg tcagacgaga attatcaatg gcttgagata gactctattc tttacccggt
      601 cgacgatgta gtcgttggaa aatccgacgg cacggtgatc gcctgccagt gcaaaaaaaa
      661 tcatactgac ttcgaagcat ggtcaataaa tgatcttgcc agcgaacttc aaaaagccgt
      721 cagactgctg agccagcatc gaaacataga agttcgcttt tattcgcgca ataattttgg
      781 tcccatcgga aaactaaagg aatattgctc aacccagcgc gacgcactca gctacaaaac
      841 cgaccttggc gccatgaatc gaagtacaga taacaatctg tctaatttga tctccgctgt
      901 agccccgggc ttttcaaact acgaatttct ctcccgcaca gagttcgaaa ccagcaacac
      961 cttggatcga atggcatccc agctacgtga gcgcttacgt aatatagcca gcaatccaga
     1021 cgccgcattc aatgcactct gggtccatct cgatcaatta ggcgcgcgga tgagcgtcgg
     1081 cgactcatct gcaggtgcac agcatcgact cattaaacat gatctggagg ccattcttaa
     1141 taaggccgga gcgctgctag cacctcccct gaatcttgac gatatacgcg catcgttttc
     1201 aaacacctct gccatcggtc gatcttggcg tcgcgatgta gcgggcaaac ggatagtcac
     1261 cccggtgatt aacgaactgc tggctgcgat caatgccaaa aagcgcgcca tcttgctgac
     1321 cggcctgcct ggctcaggaa aaacctgtgc gatgctggaa ctacaggaag cattggagca
     1381 gcgcgcacaa ctccactccg atatcgttcc actcttcatc caatcgcgtg aattcgccga
     1441 cctggctaca tcgcatgatc gacaaacgct tggcttgtct gagcaatggg taaaccaagc
     1501 tgcccgcatg gcagagcgtg cgtgggtcgt tgtggtgctc gactccctgg acgtgttatc
     1561 catcgcgcgg gatcatagaa tactcaccta ctttctagcc caaatagaca gacttctgtt
     1621 ggtcccacag gtgaccgtag tcgccgcttg ccgcgatttc gacaagcaat atgatcgtcg
     1681 aattgcggaa agagaatggg attccgaaat taaatgcctc cctttagact ggaataccga
     1741 cgtcgcgccc ttgcttgaca agctagcaat ttcatcggcg gacattgacg cagcaacacg
     1801 agaactgatt cgaaaccccc gagaactagc cttgttcgtc gagttggcac tgagaggagg
     1861 cggatttagt gtcgtcacca gccaggcact cgcacaacgc tatctcgacg tcattgtgcg
     1921 agcggacacc tcactcggcg acactgcaat acaggcgatt gaagccgtcg cccaaagaat
     1981 gctcgaatca cgtcgcctca ccgttcctca ccaacaattt caatcgcccc aggaaatcct
     2041 gcgttcgttg ttcagcctaa acatcttaca agacactcaa gatggcaagc tgacctttgg
     2101 gcatcaaaca ctgcttgatg tattggtcat cagccgtgcc ttgcgcaagg gcgtcacgct
     2161 gaacaaattc atacagagct tgcctcccgt gccatttgtg cgcccctgca ttcgcagttt
     2221 cgttgcgcag ctggccgtag gagagcggcg cgagttcaga aagcaacttc gggcagtatt
     2281 aactggcaac tccgcctttc acatccggcg tctcgttgct gaatcgcttt ccgaacagcc
     2341 acctgaggat gacgattggc ctttgatgcg tgaactgagg gaaaagcatc gcgatgtatt
     2401 tcaggctatc tattactcgg ccaagaccat tgaatggcat tatttttggc tcaagcatct
     2461 ggtgcctgtt ttaaaagcag cgcaagatgc tgaaggctac gccagccatg cctatcggat
     2521 gacgcaatgg aagaatgacg acacgcaggg cgtcttgctc tactggcaag aggctctgtc
     2581 gctggagtgg atcgatgcaa gccgtatcgc tgagcaactt ccgtatttta tatcggaaat
     2641 caattcggac aagctggcat tcgttgtgcc attgcttcaa caactactcg acatgccaca
     2701 gcccgatcac agcgtcctcg ggcaatccat cgcgcgctgt gtcgaagcgg gggccgtagg
     2761 ggataacgtc ctttggcgct acgcaatgaa cggggtcgat gaaaaatcta ttgcagaata
     2821 caacctccat aacagattac gttttcgcgc ccatgaattc ggcgaccgga aggacaattt
     2881 ttttgggcag cgcatgctaa aatcaatcgc attgcttgac ctggcgattg agtcaattga
     2941 aaggtggagc aatgcatgta cgttgtgtta tggcgagacc cgcataggat accgtcacgg
     3001 atttttgaat gaaacttcat acagaataga gcacagccag caagacgtgc atcacgctgg
     3061 gagcatgaac gtcctgctta gtgcggtcga ggcctccatt cttcatcacg cgatggaaca
     3121 ttcggagtgg tggactaaca accgcgaacg cctatgtttc aatcacgaag gagccttgct
     3181 ctacttcgcc gtgcaggcat gcacagcatc gccagaagcc aacatagatc tgatagggcg
     3241 catgctatct gatcgaaaca tgctcgaatt cggactgcaa tacgagctgg gatcattaat
     3301 tcgttcagca ttcacctccc tcaacagtac tacacaagat gctgcaatgg cgaccatatt
     3361 gtcaatatgg gacgaaccca ccactgagga ctccgagaaa tcttggatat taaaaaaacg
     3421 cacagagctt ctcaaaccca ttccatctca cctgcgctca gccgaagcac aggctacaat
     3481 agatgcattt gagagaacaa atggagcatt cgtccgccag ccagatattt tcatgcgagg
     3541 cggcaccgtc cacgccccat tctcatttga gatctttctt agcgcaagag acaatcaaat
     3601 attacgctta attgcgcatt acagaggaca ttcagattgg cacggaacag gcgccgactt
     3661 tctaatcggc ggcgagcgcg aagttggctg gcaacttcgg gaggcatcct ctcgccatcc
     3721 gactcgattc ttgcagttgt tgccaaccta ctgggcatac atctccgaac agtttcgcga
     3781 tgacatcatg gatggcatcg ccacatacct cgcgtatcgc catggtcagc tgcgacgaga
     3841 cgataattgg ttgccacttg aggaaccaga cgcctccact ttggctggcc atattctcga
     3901 tgaactggaa agacatccct tatactggca gatgcggcgc acagcagcca acgcattgaa
     3961 ggtgtgcgcc aacatagtac gaagcccgga cgatgccaaa cgacttgtat ttttagccag
     4021 aggatttgat ctgcttgaca aaaacgatcc catcaagggc gacaacatcg acttgataaa
     4081 cctaggcatc gacatggcaa agggaaacat tgccgaagca ctgctgaccc tggccaacaa
     4141 tctaagcgaa aacaacctcg acctccctag tctgctggaa cctgcgctag cccgctgtgc
     4201 cactgaagaa catccggcga ttcgcgccgt gatgctccta catctgcctt ccttgcaaga
     4261 caaaaacttc gaactgagct ggaagttgtt ttatctcatc atgcaagacg ctagaggtct
     4321 ttggcagctc gccgaacgtt gtctatatta tgcctaccat aatagattcg agacagttcg
     4381 gcctgtcctg gcacgccttc gcattgaggg tcgcgacaag gacctcgaaa catggggacg
     4441 catttcggct ttggccggaa tggctcggca catagatttt tttgaattac taatagaact
     4501 gaaagcatta gaaagcaccg atgcgtggcg aggagcggcc tccgtctgga caaactccga
     4561 gaacatccag catcatcgaa acgagtgctt cgccggcata gaagctggct taaatgcggg
     4621 cgaatcttgc gctttgaatg ttgcggaaca aatagacaac attttcagag acgaggccac
     4681 gccaatccct ttaccaatag agatcattcg aggttgtttt tccgcatttg aaaatgacaa
     4741 caacacagat aacaggcacc atcgccttct tggacttcac gaatggctga acgcagtttc
     4801 tcagaacgat ccagacttat cgctcgccgt aacaaagctc tacgtcaatt atgctagccg
     4861 ttgcggacca catatctacg accataagaa cagtctcacc cagttgatga cccgcctctt
     4921 cgctgaggcc gaggagcgag aggagtccga cgaaggagca atgctaagaa gcgtggtgga
     4981 gattcaagac gcgttacttt cacttggggt caatggggta gcggattggc taaaagcagc
     5041 cgaacgcccc tgaatccccc cgaaagcagt agtctcccat catcctcagt gcgcaaccag
     5101 gtagttgatt acagctcgtt caatacgcca agcggatgat cgctttagct gaaaggcctc
     5161 cgttcaatcc atgcaatatc agggattctt cgcgcggaat gccagtactc gcaccgcgcg
     5221 tcgagcaaga actcctcctg cgatgttctg aatcgccccc gccccccttt acatccccgc
     5281 caacctgtgt acaatgcgcc cctcttcacc acctgcccag gtggcggaat tggtagacgc
     5341 actagtttca ggtactagcg ggtaactccg tggaggttcg agtcctctcc tgggcaccac
     5401 agtgaagatc gctgtgtccg gtcgcggctg tgcggcgtcc gggcgggcaa tgcggagtgg
     5461 tagttcagtt ggttagaata ccggcctgtc acgccggggg tcgcgggttc gagtcccgtc
     5521 cactccgcca tctttctcgc gagcggcagt agctcagttg gtagagcgca accttgccaa
     5581 ggttgaggtc gagagttcga gactcttctg ccgctccagt ctttccggtc tccggcccgt
     5641 tctgccggtg ctttcccgct ttctttccat acgcccctgc acagcctgtt gtggcccgtg
     5701 cgcggtcggc gcgcgcctgt gcgcggcgtt cagccgatgg tgcccggctt gttgccgagc
     5761 atggcacgca ggttggcgag
//
LOCUS       DAOQLC010000195         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6076_length_5779_cov_19.218379, whole genome shotgun sequence.
ACCESSION   DAOQLC010000195 DAOQLC010000000
VERSION     DAOQLC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6076_length_5779_cov_19.218379"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="PL000_19975"
     CDS             complement(<1..1169)
                     /locus_tag="PL000_19975"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91214.1"
                     /translation="MNTQYKLYPYRWVVLAAFAFINLTIQTLWIAYAPITGPAAKFYG
                     VTDLQIGYFAMSFMIAFVPLSIPVSWLIDTYGFRIAVSIGAILMGIFGIVRGFAGANY
                     TLALWSTFGLAAAQPFLLNAWTKVPANWFAMEERATAVGIVTLGNLVGTALGMVLTPI
                     LLESMTIPNIQLMYGGVAAFSAVLFVLLARETPPTPPCAPGQEVRALMLDGLKYSFTV
                     KAFWFTLLVSFIGLGIFNGVTTWIEGIIRPRGFSPTDAGTLGALMIVGGVIGAVLMPA
                     LSDKQRKRQLFLYIAFLGSIPGMAGLTYATSSTLLYASAFVLGFFLTSAMPIAMQYAA
                     EVTHPTPEGTSNGLIQLFGQGAVVFVYIMEAMKSADGSFTPALLLAMGLLLASLF"
     gene            1437..>4567
                     /locus_tag="PL000_19980"
     CDS             1437..>4567
                     /locus_tag="PL000_19980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC91215.1"
                     /translation="MSNETLSQLQVELKEAQQKIVDLEAQLRAVNPGKIPVSKNNGGF
                     VSGEQANDEARREKEKVNALFLNAPLPKNVEDASQESEAKYRLLFEMMTQGVVFQDEA
                     GRILHANPAAEKILGLTVEQMQGRSHVNKDWPSIHEDGSALSAEEHPVAIVMKTKQPV
                     HNMIMGVFNPLRKSYIWLNINSVPRFLEGGTSPYQIFTTFEDITERKKIEFALRESEA
                     KYCSLIDSQESAISTIDADGVIHYMNSRGAAPFGTPETVAGKKLYDLFPLQVAEWQMS
                     QIRQVIESGKGLVHEYQNLVAGRPNWRRVSIQPIINADAGVRLATVNSLDITERKRAE
                     EELRNSERKYRDLLNGMNDTVWVIDDDLSILDVNTAAVSTLGYTREELLSMRVPDFDG
                     SLSPDAIQNLVNTMPADKFQVFQTEHTTKDGRKLPVEISSSLVTYNGKTAIMSIARDI
                     SERMQAEEKIRESESRYQQFVSLALEAISRTEFENPINTSLPVEQQIDLVYENAYIAE
                     CNQAMADMYDTTVESLTGKRIVDIHGGGENLVNRDAIRKFIAGGYQSINNETKEYSVN
                     GAPVWILNNTVGIVRDGMLLRLWETSIDITERKRAQEDLYLAEQRYRAMIEHAPDGIV
                     LITVDGKFKYASPSVERIFGYVQEDLQALNSVSMTHPDDLPKVLRELQKLLEDQSYIP
                     TVQYRFLHKNGEWRWIESTFSNLLATPNVEAIIINFRDIHERRLADEELKKSQSLLKE
                     SQRIGRIGYMEWSVNSPNLICSDEIYDIFNIPSGSPVTKDLISTMLRPDESEKLRELD
                     HQATIHRADINYEYSVLTRDGNERCIHQTGKITYDETGNPIRKMLIVQDITERKQAEK
                     QLQRNEQVLRLFVEHSPAAIAMFDREMRYIIASQRYLIDYRLGDQSLIGRSHYEVFPE
                     MDEGRKAIHRRCLAGEVVKHEGDPLPRVDGTLDWVRYELRPWYEVDGSIGGLIFFSEV
                     ITERKQAEEKLRASEEKYRGLIESLGSIVATVDAQGKLLYLNDLAASSLGGSAAELTG
                     RSMYDYFPPKYAE"
     assembly_gap    4568..4667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4668..>5779
                     /locus_tag="PL000_19985"
     CDS             <4668..>5779
                     /locus_tag="PL000_19985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91216.1"
                     /translation="LKNNGRVTGLEFNMRRKDGSTFPVLLNATAVFDEHGNYLRSRST
                     IFDNTERKKAEDALRVSHDQLAAANIDLEKAIRVKDEFLASMSHELRTPLTGILGLSE
                     AIQLGTYGGLNEKQKKTLQSINDSGNHLLSLINDILDLSKIEAGKLEMQITPCSLGDI
                     CQSSLQLTKGMAHQKHQFVSYSPPAEAILLNADARRAKQILVNLLSNAIKFTPENGEL
                     GLDVEPDAVNHRVRLIVWDKGIGIKAENLEKLFKPFTQIDSSLAREYSGTGLGLSLVQ
                     RLVKLHNGTVEVESVFGEGSRFIVSLPWMPDISASNSPKADSTQLTSSPSNPTAPDAS
                     PVILVADDNPVLLTMLADFLEASHYRAVKAESGRDVL"
BASE COUNT         1542 a         1281 c         1540 g         1316 t
ORIGIN      
        1 aagaacaggc ttgccaatag taatcccatt gcaagtaaaa gggcaggggt gaaggaaccg
       61 tcagcgcttt tcatggcttc catgatgtag acgaagacca cggctccctg cccgaagagt
      121 tggatcaaac cattggaggt gccttcgggg gtggggtggg tcacttctgc ggcgtattgc
      181 atggcaatgg gcatggcgct ggtgaggaag aatcccaaaa cgaaggcaga ggcgtataac
      241 agcgtggacg atgtcgcgta ggtcagacca gccatccctg ggatggaacc gaggaaggcg
      301 atgtagagga agagttgccg ctttcgttgc ttatccgata aagcaggcat caacacggct
      361 ccgatcacgc cgccgacgat catcaatgcg ccgagcgtgc ccgcgtctgt gggagaaaat
      421 ccgcgcggac ggatgatgcc ttcgatccat gtggtgacac cgttgaagat tcccagcccg
      481 ataaaggaga cgagcagggt gaaccaaaag gctttgaccg tgaatgaata cttcaaacca
      541 tccagcatca gagcgcggac ttcctggccc ggcgcgcagg gcggggtggg cggggtctcg
      601 cgtgccagca ggacgaagag tactgccgag aatgccgcaa ctccaccgta catgagctgg
      661 atgtttggga tggtcatgct ttcaagcagg atgggtgtca ataccatgcc gagggctgtg
      721 ccgacgagat tgcccaaggt gacgatgcca acagcggtgg cgcgttcttc cattgcaaac
      781 caattggcag ggactttcgt ccacgcgttg agcaggaagg gctgggcggc ggcgagaccg
      841 aaggtgctcc acagggcgag ggtgtaattc gcgccagcga atccacgcac gatgccgaag
      901 atgcccataa ggatcgcgcc gatgctgacc gcgatgcgga agccgtaggt gtcaatgagc
      961 cacgaaacag ggatggagag cggcacaaag gcgatcatga aggacatggc gaagtagccg
     1021 atctgcagat cggtgacacc gtagaatttg gctgcgggac ctgtgatggg ggcgtaggcg
     1081 atccacaggg tttgaatggt aaggttgatg aaggcaaaag ctgccagcac cacccagcga
     1141 taagggtaga gtttgtactg tgtgttcata ttgtttcctt ttggctgggg ccaatcatat
     1201 caggtacggt aaattttgcc ttgtgtgttt tcacctttta tgaaaaactg tcaaaatctt
     1261 gatcgataaa catggtcgct tataacagat ttgttatgcg tttcagagtt gaaaagcaag
     1321 cgttattaag taatattacg gatggacaca gcctgtatag tatattattt ttgccttatt
     1381 tataccttct tgaagcatgt ttcttgggtg cttttttcta taataaggaa aaaattatga
     1441 gcaatgaaac cttgtctcaa ttgcaggtgg aattgaaaga ggcgcaacag aaaattgtcg
     1501 accttgaagc gcaattgcgg gctgtaaacc ctggcaagat acctgtgtca aaaaataatg
     1561 gtgggtttgt cagtggggaa caggccaacg atgaggcccg aagggaaaag gaaaaagtca
     1621 atgcgctttt tcttaatgcg ccgcttccaa agaacgtgga agatgccagc caggaaagcg
     1681 aagcaaaata cagattgctt tttgaaatga tgacccaagg ggttgttttt caggatgaag
     1741 ctggccgaat ccttcatgcc aacccggctg ctgaaaaaat attggggctg accgttgaac
     1801 aaatgcaggg acgttcccac gttaataaag attggccaag catacacgaa gatggctctg
     1861 ctctttcggc ggaggaacac cccgtggcga ttgtcatgaa aaccaaacag cctgttcaca
     1921 acatgatcat gggcgtgttc aatccattga gaaaatccta tatctggttg aacataaact
     1981 cggtaccgcg ttttcttgag ggcgggacca gtccgtatca aattttcacc acatttgaag
     2041 atattaccga aagaaaaaag attgaattcg ccctgcggga gagtgaggca aaatactgca
     2101 gcttgattga cagccaggag tctgccattt caacaataga tgcagacggt gttattcatt
     2161 acatgaattc tcgtggtgca gccccgttcg gtacacctga aaccgtggca ggaaaaaaat
     2221 tgtacgatct attcccgctt caggtagcag agtggcagat gagtcaaatc cgtcaggtaa
     2281 tcgaatctgg aaaagggctg gtgcatgaat atcagaattt ggttgcaggc aggccgaatt
     2341 ggcggcgcgt gagcattcag ccgatcatta atgcagatgc tggcgtaagg cttgcaaccg
     2401 tcaattcttt ggatattacc gagcgaaagc gcgcggagga ggaattacga aatagcgaaa
     2461 gaaaatatcg tgacctgctc aatggaatga acgacacggt gtgggttatt gacgatgacc
     2521 tgtcgatcct ggatgtgaat accgcggcgg tatccacgct tgggtatacc cgtgaagaac
     2581 ttctctccat gcgtgttcct gattttgatg gctccctttc tcctgatgcc atacagaacc
     2641 ttgtcaatac catgcctgcg gataagtttc aggtatttca gacagagcac acaacaaaag
     2701 atggccgaaa actccctgta gagatcagtt ccagtctggt gacatataac ggcaaaaccg
     2761 ccattatgag cattgcccgt gacatcagtg aacgcatgca ggcggaggag aagatacggg
     2821 aaagcgagtc tcgatatcag caatttgtct cccttgctct tgaggcgatc tcacgtacgg
     2881 aatttgaaaa cccaattaat acatccctgc ctgttgagca gcagatcgac cttgtttatg
     2941 agaatgccta tattgccgaa tgtaatcaag ccatggcgga tatgtatgac acgacggtcg
     3001 agtctcttac tggaaagcgg atcgttgata ttcatggcgg tggagaaaat ctggttaacc
     3061 gcgatgcgat cagaaagttc atcgctggcg ggtaccaatc cattaataac gagaccaaag
     3121 agtattcagt caatggagca ccagtttgga tattaaataa cacggttggc atcgtccgcg
     3181 atgggatgct tttacgtttg tgggagactt cgatcgatat taccgagcga aaacgcgccc
     3241 aggaagatct gtatctggct gagcagcgct atcgggcaat gatcgagcat gcaccggatg
     3301 gtattgtttt gatcactgtg gatggaaaat tcaagtacgc cagcccatcg gtggagcgga
     3361 tatttgggta cgtgcaggaa gacctgcaag cattaaactc tgtatccatg acacaccctg
     3421 atgatcttcc aaaggtattg agggaattgc aaaaattgtt ggaagatcaa tcttacatcc
     3481 caactgtgca ataccgtttt ttgcataaga acggggagtg gcgctggatc gaaagcacat
     3541 tcagcaacct gcttgcaacc ccaaatgtgg aagccatcat cattaacttt cgggatatcc
     3601 atgagcgcag gctggcagat gaggaactta agaagagcca aagcctgctt aaggaatccc
     3661 agcgtatcgg acgaatcggg tatatggaat ggagcgtgaa ttctccaaac ttgatctgtt
     3721 cagatgagat ttatgacatt ttcaacatcc cgtctggaag ccctgttaca aaagacttga
     3781 tcagcaccat gttgaggcct gatgaaagtg aaaaattgcg ggaattggac catcaagcga
     3841 ccattcatcg tgccgatata aactatgagt attcagtcct tacaagggat gggaatgaac
     3901 gatgtattca tcaaacaggt aagatcacct acgatgaaac tggcaacccg atccgcaaaa
     3961 tgcttattgt gcaggatatt accgaacgta aacaagccga aaagcaattg cagcgcaatg
     4021 aacaggtcct gcgtttgttc gtggagcaca gcccggcagc cattgccatg tttgaccggg
     4081 aaatgcgcta catcatcgcg agtcagcgct acctgatcga ttatcggctt ggtgaccagt
     4141 ccttgatagg acgctcgcac tacgaagtat ttcctgagat ggatgaaggt cggaaggcga
     4201 tccatcgtcg ctgcctggct ggggaggtgg taaaacatga aggagacccg ctcccaagag
     4261 ttgatggaac tttggattgg gtccgatatg aactccgccc ctggtatgag gtagatggca
     4321 gtattggcgg cctcatcttt ttctcggaag tgatcaccga acgcaagcag gcggaagaaa
     4381 agttgcgcgc cagtgaggaa aaatatcgag gcttaataga gagcctgggc agcatcgttg
     4441 ccacggtgga tgcccaggga aagctcctat acctgaatga cctggcagcc agcagccttg
     4501 gcggatccgc tgctgaattg acaggcaggt ccatgtatga ttacttcccg ccaaaatatg
     4561 ctgaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta aagaataatg
     4681 gcagggtaac cgggcttgag tttaatatgc ggcgcaagga tgggtcaaca ttccctgtgc
     4741 tgctcaatgc aacggctgta tttgacgagc atggaaacta ccttcgcagc agatccacca
     4801 tctttgataa cactgaacgt aaaaaagctg aagatgcact tcgggtcagc catgatcagc
     4861 tggctgccgc caatatcgat ctggaaaagg cgatcagggt caaagatgaa ttcctggcca
     4921 gcatgagcca tgaattgcgc acgccgctta caggcatcct cggtctttcc gaagccattc
     4981 aacttggaac ttatggcggc ttgaatgaaa aacaaaaaaa gaccctccaa agcattaatg
     5041 acagcggcaa tcatttgttg tcgctcatta atgatattct ggatttgtcg aagatagaag
     5101 ctgggaaatt ggaaatgcag atcacgccgt gttcgcttgg tgatatttgc caatccagct
     5161 tgcagcttac gaaaggcatg gcccaccaga aacaccaatt cgtcagttat tctccgcccg
     5221 ccgaagccat cctcttgaat gcggatgcga ggcgagcaaa acagatcctg gttaatttat
     5281 tgagcaatgc gatcaagttc acacctgaaa acggcgagct tgggcttgat gttgagcccg
     5341 atgcggtcaa tcatcgggtg cggctgatcg tttgggataa agggatcggg atcaaggcgg
     5401 agaaccttga aaaattattc aagccattca cgcagatcga tagcagcctc gcccgtgaat
     5461 attccggtac aggccttggc ctttcgctgg tgcagcggct tgtgaaattg cacaatggga
     5521 ccgtggaagt ggaaagcgtc tttggagagg gcagccgctt tatcgtttcc ctgccctgga
     5581 tgccagacat ttccgcttca aactcaccca aagcggacag cacccaattg acatcttctc
     5641 cctctaaccc aactgcccct gatgcttccc ctgtgatcct ggttgcagac gacaaccccg
     5701 tacttctcac tatgttggcc gattttttgg aggcgagtca ttatcgtgct gtaaaggcag
     5761 agagtggcag ggatgtcct
//
LOCUS       DAOQLC010000196         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6183_length_5716_cov_15.191309, whole genome shotgun sequence.
ACCESSION   DAOQLC010000196 DAOQLC010000000
VERSION     DAOQLC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6183_length_5716_cov_15.191309"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..4872)
                     /locus_tag="PL000_19990"
     CDS             complement(367..4872)
                     /locus_tag="PL000_19990"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC91217.1"
                     /translation="MLKTIKQFFTPPVFPDEDDKTRNAQILYALFASMMGILALLMLA
                     NIFVFVAKAIVSILLFILFIWILVARALAQSGKILLASRIFVSGLWILTTFQLLLSGQ
                     LSAAALAFHVSNLSIAGVLLKKRSAITLAVMSSLVGLGMVILEITGYSLHRYFPNPPI
                     VYWITWVIAFIMTIIPLNLTVQSAGDALARAQKSERRYVTLFNKAPMMYLIIRNEGDI
                     PIISDCNFTFLKNLGYTREQVIGQPLANFYTPQSRLMLLKEGGFQSSLQNEVTTSIER
                     DFVAQDGHILHTLVRTASEIDESGQSIGVLAMYLDITERKQAEEALRESETKLQTIFA
                     RSRDAIGVSKSGVHIFVNPAYVFMFGYESAEELIDTPVINLIAPESREMIIHNIQDRD
                     KGLAVPSDYETVALRKDGSTFILDVRISTYILQGEQYTLVILRDITERKRAEEALQIK
                     EWAIRSSINALALSDLDGSLIYVNPAFLALWGYQTEQEVLGRSAIEFWQVQENADQVI
                     SALRTQGGWMGEMVATRKDGTLLDVEVVASMVADNAGKPLCMMGAFVDITDRKRAEVE
                     LTIAKEKAEENEKKLREVLDNTKVQLWAFDGTRYNYVSKEWYDFTGQTLDTPLTIDLW
                     VSAVHPDDREPSGKIWLENWERKTAHDNYFRLRRQDGVYRDFYCHAIPIFDSQNKFKY
                     FHGFNVDITERKQAEEKLRHAENRLELFFSQSLDGFFFMLLDEPIQWSDDIDKDKTLE
                     HVFAHQRITKANDAMLSLFGTTRKQFIGLTPKDLYPGDVEQRKHFWGDLFDKGHLQLL
                     TAETKSDGSIMWLEGDYVCMYDTDGRLLGHFGIVRDVTERKQAEAVTAARARILQFSL
                     THSLPDFLQNVLDECELLTSSSIGFFHFMEADQNTLSLQNWSTRTLGEFCTAVGKHLH
                     YPLSQAGVWADCARERQAIICNDYAGAINSKGMPDGHAEIKRFVSIPILRGENVVAII
                     GIGNKAVDYDREDVEIVTQLADLSWDITERKRAEDSLWESETRFRRLANNAPDIIFRY
                     NFTPEMKLAFINPAVEKITFYTPEECYADPLLMLNMAHPDDAMMMAEYLQSRQTPDKP
                     LIMRWLDKYGTIHWMESRLVPIYDEADQLVAVEGITRDITERKRAELQIEEQLKRLNA
                     MHTIDTFIKSSFDLKNTLNILLTEVATQLKVDAASILLFNKDTLTLNYSAGYGFRSIA
                     IQHTKLRYGEGFAGRLLHDRKIIRIPDLTKTESELAKALALKGDNFTSYMGVPLIAKG
                     QTLGVLEIFQRAELTPDTDWFDFLEMLADQAAIAIDNMQLFESLQRSNLDLTLAYDAT
                     IEGWSRALDLRDKETEGHTQRVTSLTIQLAQQMGVPDADILHIRRGALLHDIGKMGVS
                     DNILRKPGPLTEAEWQEMRQHPLYAYKMLAPISYLNPALDIPYCHHEKWDGTGYPRGL
                     KGEEIPLVARIFAIADVYDAITSDRPYRAAWAKEKALDFIREQSGRHFDPNVVKYFLE
                     MIKDEVK"
     gene            complement(5007..5408)
                     /locus_tag="PL000_19995"
     CDS             complement(5007..5408)
                     /locus_tag="PL000_19995"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC91218.1"
                     /translation="MTDYVADTHALIWYLENSPRLGSQARVAFDACDRGEIQIYVPTI
                     CLVEIVYLMEKGRISPEMKSMLDEELKSGESGLVLADLNMDVVDQIANVLRDDIPDLP
                     DRVIAATALALNLPLISRDRKIVLSGLDTIW"
     gene            complement(5408..5638)
                     /locus_tag="PL000_20000"
     CDS             complement(5408..5638)
                     /locus_tag="PL000_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91219.1"
                     /translation="MVTPTSTDVLKMLRQLPPSEQLRIISLALPEIEKSLGRKARARK
                     SLCGLWSGAGITPKDIAETHKGMLSNFPRSDI"
BASE COUNT         1454 a         1427 c         1327 g         1508 t
ORIGIN      
        1 tttcagacgc gtacattcgc atccgcgtgg cgtttattga tgcggcacgc aaacgccccg
       61 atgaattcaa aaaacgatta aggcattttg ttgaagtaac cgcaaagaac aggcagatcg
      121 ggtttggggg aatcgaaaag cattattgac ccccctccgt tggcacccaa atattctcga
      181 cctgcgttgc ttcgtgcagg aactcgtgac cttcgccttg ctcgcggtcg agccaatcgc
      241 ggggcagacc gtagcctgtc catgtgcgct ttatgttcgc agtagactta ttctcgaaat
      301 gtaactacct tcctctgagc aatcaggttt gatgtaaaca catataacta ttttgcaaat
      361 aaaagtttat ttcacttcat cctttatcat ttccaagaaa tacttgacaa cattggggtc
      421 aaagtgcctg ccgctttgct cacggataaa atccagagct ttttcctttg cccacgcggc
      481 acgataagga cggtcactag tgatggcatc gtacacatcc gcaatggcaa agatacgcgc
      541 cactagtgga atttcttcgc ctttcaaacc gcgcggatac cctgtgccat cccatttttc
      601 gtggtgacaa taggggatat ccagtgcggg gttcaagtaa gagatggggg cgagcatttt
      661 atacgcatat agaggatgtt ggcgcatttc ctgccattcc gcctcggtca atggaccagg
      721 cttacgcagg atattatccg acacacccat tttgccaata tcgtggagca atgcaccgcg
      781 gcggatgtga agaatatcgg cgtcgggaac gcccatttgc tgtgccagtt gaatggtcaa
      841 ggatgtaaca cgctgggtat gcccctcggt ctccttgtcg cgcaggtcca atgcgcgcga
      901 ccagccttcg atagtggcat cgtaagccaa ggtaaggtca agattggagc gttgcaggct
      961 ttcgaataat tgcatgttgt caattgcaat ggctgcctga tctgcaagca tttcaaggaa
     1021 gtcaaaccag tcagtgtctg gggtgagttc agcgcgctgg aagatttcca gtacacccag
     1081 ggtttgcccc ttggcaatca gcggcacgcc catgtaagaa gtaaaattat cacctttcag
     1141 tgcaagggct ttggcaagtt cgctctctgt tttcgtcagg tctggaatgc ggatgatttt
     1201 cctgtcatga aggaggcggc ccgcaaagcc ttcgccgtat ctaagttttg tgtgctgtat
     1261 ggctatagat cggaacccat agccggcgga gtagttaagc gtaagtgtat ccttgttgaa
     1321 taaaagtatg gaggcggcat ccaccttgag ctgggtggca acctcagtca gtaaaatatt
     1381 gagcgtgttc ttcaagtcaa aactggattt gatgaaagta tcaatggtgt gcatggcgtt
     1441 cagccgcttg agttgttcct cgatctgtag ttctgcccgt tttcgttcag taatgtcacg
     1501 ggtgatacct tccaccgcca caagttgatc cgcttcatcg taaatcggca caagacgact
     1561 ttccatccag tgaattgttc cgtatttgtc cagccagcgc atgatcaaag gcttgtcagg
     1621 agtttggcgc gattgcaaat actcagccat catcattgca tcatcgggat gcgccatatt
     1681 gagcattaac agcgggtctg cataacattc ctccggggtg tagaaggtga ttttttccac
     1741 tgccgggtta ataaaagcca gcttcatctc tggggtaaag ttataacgga aaataatatc
     1801 gggggcgtta ttggccaacc gtcggaaacg agtttcgctc tcccacaacg aatcttctgc
     1861 ccgtttgcgc tcggtaatat cccagctaag gtctgccaat tgggtcacaa tctcgacatc
     1921 ctccctatca tagtccacgg ctttgttacc aatgccaatg atggctacaa cgttttcgcc
     1981 gcgcaggatg ggaatcgaca caaaacgttt gatctccgca tgcccgtctg gcatgccttt
     2041 gctgtttatc gccccggcat aatcattgca aatgatggct tgtcgttcac gggcgcaatc
     2101 agcccagact cccgcctgac ttaatggata atgcaggtgt ttgcctacag cagtacaaaa
     2161 ctcacccaaa gtgcgtgtgg accagttttg tagcgacaat gtattctggt cggcttccat
     2221 gaaatggaaa aaaccgatcg aactggatgt gagcaattca cattcatcga ggacgttttg
     2281 caagaaatct ggaagcgaat gtgtcagtga aaattgcaag atgcgcgcgc gtgcggcagt
     2341 aaccgcctct gcctgcttgc gttcggtgac atcgcgtacg atgccaaaat gccccaaaag
     2401 acgtccatcg gtatcataca tgcacacata atccccttca agccacatga tggaaccatc
     2461 agacttggtc tcagcggtaa gcagctgaag atgtccttta tcaaaaagat caccccaaaa
     2521 atgcttcctt tgttcgacat cgccgggata aagatctttc ggtgtcagcc caatgaactg
     2581 cttacgagtt gttccaaaca gagacaacat ggcatcgttt gcttttgtga tgcgctgatg
     2641 agcgaaaaca tgctccagtg ttttatcttt atcgatatca tcactccatt gaatcggctc
     2701 gtctaaaagc atgaagaaaa agccatcaag agactgtgag aaaaaaagtt caaggcgatt
     2761 ttccgcatgg cgcaatttct cctccgcttg tttgcgttca gtgatatcca cattaaagcc
     2821 atgaaagtat ttgaatttat tttggctatc gaaaatcggt atggcatggc aataaaagtc
     2881 cctatatacg ccatcttgtc ttctcaatcg aaaataatta tcgtgcgcgg ttttcctttc
     2941 ccaattttcc aaccatattt tccctgaagg ttcgcggtca tcaggatgta ctgccgaaac
     3001 ccacagatca atggtaagcg gggtgtctag tgtttgcccg gtgaaatcgt accattcctt
     3061 gctcacgtag ttatacctgg ttccatcaaa agcccacaat tgaactttgg tgttgtccaa
     3121 cacctcgcgt aacttttttt cattttcttc tgccttctct ttggcaatgg ttaattccac
     3181 ttccgcccgc ttacggtccg tgatgtcgac aaatgcaccc atcatgcaaa gcggtttgcc
     3241 ggcattgtct gcaaccatgc tggctaccac ttcaacatca agcaatgtgc catcttttcg
     3301 cgtggctacc atctcgccca tccagccgcc ttgagttcgt aatgctgaga tgacttgatc
     3361 cgcattttct tgtacctgcc agaactctat agccgacctg cctagaacct cctgctcggt
     3421 ctggtatccc cacaaagcaa gaaaagcggg gttgacgtat atcaggctcc catccaagtc
     3481 agatagggca agggcattaa tggaagaccg gatagcccac tctttgattt gcaacgcctc
     3541 ctctgcccgc ttgcgctcgg tgatgtcgcg cagaatgact agtgtatatt gttcgccttg
     3601 caatatgtag gtggaaatac gcacatccaa aataaaagtt gatccgtctt ttcggagcgc
     3661 gacggtttca taatcagaag gcactgctaa acctttatca cgatcttgaa tattatggat
     3721 gatcatctcg cggctttcag gcgcgatgag attgataacg ggagtgtcaa tcaactcctc
     3781 cgcgctttca tatccgaaca tgaatacata cgccggattg acaaaaatat gaaccccgct
     3841 tttggaaacc ccgattgcat ccctcgatct tgcaaagata gtctgtagtt tcgtttcgct
     3901 ttcgcgcagc gcctcttccg cctgtttgcg ttctgttatg tccagataca tcgccagcac
     3961 accaatggat tgccccgact catcaatctc agaagcagtc cgtaccagtg tgtggaggat
     4021 atgcccatct tgcgccacaa aatcccgctc aatcgacgtt gtcacctcat tttggagcga
     4081 gctctgaaaa ccaccttctt tcaatagcat caagcgtgat tggggagtat agaaattagc
     4141 cagtggctgg ccgatcactt gttcacgcgt atagcccagg ttttttagga atgtaaaatt
     4201 gcaatccgaa ataatgggta tgtctccctc attgcgaatt atcaagtaca tcatcggcgc
     4261 tttgttgaac agggtcacgt agcgtcgttc gctcttctgc gcgcgtgcca gcgcatctcc
     4321 tgcgctctgg acggtcaggt tcagcgggat aatggtcatg ataaaagcaa tgacccaggt
     4381 gatccaatat acaattgggg ggttgggaaa gtaacgatgc aacgaataac ctgtgatttc
     4441 aagaattacc atccccaacc caaccaggct gctcatcacc gccagtgtga tggcagaacg
     4501 cttctttaaa aggacaccag caatggacaa gttcgacaca tgaaaagcga gagcggcagc
     4561 agatagttgt ccagacagta aaagttggaa tgtagttaga atccacaatc cgctcacaaa
     4621 tatccggctg gcaagcagga ttttgccgct ttgcgccagt gcgcgtgcaa ccagtatcca
     4681 aatgaaaagg ataaacagca aaatactcac aatggctttg gcaacaaata caaaaatgtt
     4741 ggcaagcata agaagtgcca aaatccccat catgctagca aatagcgcat agagtatctg
     4801 cgcattacgg gtcttgtcat cctcatctgg gaagactggc ggcgtaaaga attgcttgat
     4861 ggtttttagc atagggcacc tcttattttg aaacaagggt acttttgttg ggcgtgcttc
     4921 gcgcagggct atctattcac agagtagtta gcgacagggg tcactccccc gtcggcaccc
     4981 agatattctt cacctgcgtc gccaaattac caaatcgtat ccaaaccact caaaacaatt
     5041 tttctgtcac ggctaatcaa gggtaaattt aatgcaagtg cggttgccgc aataacccga
     5101 tctggcaagt caggaatatc atcacgcagg acgttggcaa tctggtcaac tacgtccata
     5161 ttgaggtctg caagtacaag tccgctttcg cctgatttca attcctcgtc caacatggat
     5221 ttcatttcag gggaaatgcg tcctttttcc atcaggtaga caatttcaac cagacagatg
     5281 gtcggcacgt aaatttgaat ctcacctcgg tcgcatgcat caaaagcgac gcgggcttgt
     5341 gagccaaggc gagggctgtt ttccaaatac cagatcagag catgagtatc ggcgacgtaa
     5401 tcagtcatca aatatcactc cgcgggaagt tgctcaacat ccccttgtgt gtttcagcaa
     5461 tatccttggg agtaatacca gcacccgacc acaatccgca cagggatttg cgtgcgcggg
     5521 ctttcctgcc gaggcttttc tcgatctctg gcaaggcaag cgaaataatc ctcaactgtt
     5581 cgcttggtgg tagttgacgg agcattttca aaacgtcggt tgatgttgga gtaaccataa
     5641 atcacctcac agaataatta tacctgtcac tctcccgtcg gtacccagat attcttgacc
     5701 tgagtcgcct cgtgca
//
LOCUS       DAOQLC010000197         5714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6191_length_5714_cov_25.006008, whole genome shotgun sequence.
ACCESSION   DAOQLC010000197 DAOQLC010000000
VERSION     DAOQLC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6191_length_5714_cov_25.006008"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PL000_20005"
     CDS             <1..402
                     /locus_tag="PL000_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91220.1"
                     /translation="NFMNTKILMTSSALVLGAAGIFALFMPDLVLSAQGATATEAMSM
                     LVQLMGALYFAFALMNWTAKDGVIGGIYARPVSLGNFAHFFAGALLLVKAQFSSEFSW
                     LGFVVMLVYVFYALCFYWLVFRATGIKPPAA"
     gene            complement(403..1215)
                     /locus_tag="PL000_20010"
     CDS             complement(403..1215)
                     /locus_tag="PL000_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91221.1"
                     /translation="MKNDAILPLTRMITAVVVFFLVTAFGILFFLPDRTGELFAWAIK
                     PHMSSMYFGSAYLGGAWILAQTAFGKRWHRVQAVFPAVTVFTTAMLIATLLHWDRFSL
                     GTIPFMAWLILYIISPFLIPALWIYNKRTDSNLPEESDLTVSATARMVTRIFGTLVLV
                     FVLIGFISPNLIIPIWPWTLTPLTARVLCGWLSVIGTGAWAISLDPRWTGWRAILEGI
                     FIAYSLIFVAAFMNPADFSTGIFNWYTAFMGIMLIAILVFYTSMESKRRKAS"
     assembly_gap    1274..1373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1563..1949
                     /locus_tag="PL000_20015"
     CDS             1563..1949
                     /locus_tag="PL000_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91222.1"
                     /translation="MKKLIGLWIDRKRALILTLEEKKEQLKQIQSNLAAHTQFEGDTH
                     AKYFKAEDQMDRHLGEHMKKYYREIITSVRDAAAILIMGPGEAKFEFEKRLAHEHVKA
                     RVAMVETADKLTERQFTARVKKYFKS"
     gene            2098..2592
                     /locus_tag="PL000_20020"
     CDS             2098..2592
                     /locus_tag="PL000_20020"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNC91223.1"
                     /translation="MNTPKRYNPFLVTLHWLTVVLMLGAGLLSESGGDSPINFHMMLG
                     GALLVVTLIRIGVRFATKHPAQADTGNPLFNQLGQWMHYGLYLALFFILGMGALIANK
                     RNLLAVAMGTGSVTGRAGFLGDIHHLGWFLAILLILGHVGAALYHQFILKDNLLGRMW
                     FGKQ"
     gene            complement(2698..3477)
                     /locus_tag="PL000_20025"
     CDS             complement(2698..3477)
                     /locus_tag="PL000_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91224.1"
                     /translation="MNILMLKLILAPVIIGSASMAGRRWGAAVSGWLVGLPLTSGPVA
                     FFLALSHDRAFVFDSVRGTLSGGFSLVAFSLTYAWVSRKFNWVVSILSGVVVFASMTT
                     LLQNITIPFLPLFAAVIVVIFIGLRLMPKGQAVPPVSNPGGWDIPARILIGTSFILLI
                     TGIAPYIGSRLTGLLTTIPLYVSILTIFSHRHQGHAGAVNVLHGLILGLFSFAGFYLV
                     LGLLIERSSLAASFGSAILATLVVQGLSLLILRQRHIHKGE"
     gene            complement(3474..4091)
                     /locus_tag="PL000_20030"
     CDS             complement(3474..4091)
                     /locus_tag="PL000_20030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HNC91225.1"
                     /translation="MNQFKIFAPIFPYLAVGVGLFFFNNAWAALFGFHIAILLTVLVL
                     RPSLSVRTLFKSRSPKWILASVLFCSTSGIGLYFLWPVFGVIGDLSAQLLAMGLTPAS
                     WPWFLAYFSLVNPFVEEYFWRGVLGNDSLKPYFGDVVFAGYHAMILWGRVHLVFILFA
                     VSVLVSAGWLWRQISREDGGLLSASLGHMAADFTILVTIYWMAVP"
     gene            complement(4120..5043)
                     /gene="mvk"
                     /locus_tag="PL000_20035"
     CDS             complement(4120..5043)
                     /gene="mvk"
                     /locus_tag="PL000_20035"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HNC91226.1"
                     /translation="MKASAHGKIILFGEHAAVFGHPALAVPVTQVHVDVEVLDSPRTG
                     IWIHAPIIDLHAELNSLPPDHAIASVILKVIQHFGISPLPNLEVNISSTIPVAAGLGS
                     GAAVSVALIRALSAFLSQPFSDEEVNSLAYEIEKIHHGTPSGIDNTVITYGKPVYFMK
                     DKPVEIFKVGRPFTIVIGDTGIAAPTKESVGDVRRLWIRDQFTVENIFNEIAQIALIG
                     RRSIESGRTEMLGELMDHNHEYLQQLTVSSPELDSLVEAARKAGALGAKMSGGGRGGN
                     MIALVDPARAESVAAALISAGAKRTVITEIK"
     gene            complement(5040..>5714)
                     /locus_tag="PL000_20040"
     CDS             complement(5040..>5714)
                     /locus_tag="PL000_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HNC91227.1"
                     /translation="RQHPEKKNRPIASGKLPVGVAVTAAVLFVAITFVLAYLLTPSLL
                     AVIGGYFVINLAYSKWLKHVPILDVLIISTGFVLRVGAGVTLIEVERFSPWLYVVMTL
                     LSLFLGFGKRRAELALLANGAGSHRKVLDGYTLPLLDQYIMIVSGATIVAYSLYTFSA
                     PNLPENHTMMLTIPFVVYAIFRYMYLIEVKHAGGAPEEVLLSDRPFQLAMVLWGLTVL
                     AIFYLS"
BASE COUNT         1542 a         1407 c         1496 g         1169 t
ORIGIN      
        1 aactttatga acacaaaaat attaatgaca tccagcgccc ttgtccttgg ggcagcaggg
       61 atcttcgccc tgttcatgcc tgacctggtt ttatcggcgc agggagcgac agccacagag
      121 gcaatgtcca tgctggtgca gttgatgggc gcattgtatt tcgcctttgc gctgatgaat
      181 tggacggcca aagatggtgt gatcggcgga atttacgcgc ggcctgtgtc gctgggaaat
      241 tttgctcatt tctttgcagg ggcgctgctg ttggtgaagg cacaattctc cagcgaattc
      301 agttggctgg gatttgttgt gatgcttgtg tatgtgttct atgcgctgtg tttttactgg
      361 cttgtgtttc gagcaacagg gatcaaaccg ccagccgcct agttaactgg ctttccgtct
      421 cttgctttcc atgctggtgt agaaaacaag gatcgcgatc aacatgatgc ccatgaaggc
      481 ggtgtaccag ttaaagatgc ctgtgctgaa atcggcgggg ttcatgaatg cagcgacgaa
      541 gatcaggctg taggcaatga agataccttc aaggatggcg cgccatcccg tccagcgcgg
      601 gtcgagggag atcgcccatg caccggtgcc gatcacagaa agccatccgc acagcacacg
      661 cgcagtaagc ggagtcaacg tccacggcca gatggggatg atcagattcg gggagataaa
      721 gccaattaga acaaagacca gcacaagggt tccaaagatg cgggtgacca ttcgggcagt
      781 agctgaaacg gtcagatccg attcctcagg caggttcgaa tcggtccgct tgttgtatat
      841 ccacagtgcg gggatcaaga acggggaaat gatgtaaagg atcagccagg ccatgaaagg
      901 gatcgtgcca agcgagaagc ggtcccaatg cagcagggtg gcgatgagca tggctgtggt
      961 gaagacagtg accgcaggga agacggcctg tacacgatgc cagcgcttgc cgaaggcggt
     1021 ttgggcgagg atccatgcac cgccgaggta ggccgagcca aaatacatcg aagacatgtg
     1081 cggtttgatg gcccaggcaa acaattcacc ggtgcggtct ggcaggaaga acaggatgcc
     1141 gaaagccgtt accaaaaaaa atacgacaac ggctgtgatc atacgggtaa gggggagaat
     1201 cgcgtcgttc ttcatcaggt catcctttca attttcaaag agcgggtttg cgcaaaactc
     1261 agcctgtcca cgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttgttt
     1381 attgatattc atctctggca aatggttttg cgagaaccct aaacggataa ggtcaggcga
     1441 tattaccatg cctcacacag ccgcacaatc gtccatgcca gcctttggat ttgctttgcc
     1501 cctcatagat atgtatactg agattgggtc gtgttcgctt ttcaatgtca taaggaggtt
     1561 ccatgaaaaa gctcattggt ttatggatcg accgaaagag ggcattgatc ttaaccctcg
     1621 aagaaaaaaa ggagcagctc aaacaaatcc aatcgaatct tgcagcccat acccagtttg
     1681 aaggggatac tcatgcaaag tatttcaaag cggaagatca gatggacagg cacttggggg
     1741 aacacatgaa aaaatattac agggagatca tcaccagtgt ccgcgatgcc gctgccattt
     1801 tgatcatggg cccgggcgag gccaagtttg aattcgagaa acgcctggcc catgagcacg
     1861 tcaaagccag ggtggcgatg gtcgaaaccg cagacaaatt gaccgaacga caatttacag
     1921 ccagggtgaa gaaatatttc aaaagctagg tttcaaaaaa gattcccaat gacaaattgg
     1981 gaatcttttt ttacagacgg ggttgtgtgg aaattatgtg gaaatctcga aaacaacttg
     2041 attgtcattt tgagttaata aagccaatat aaaatctcgt gaaaaggaga gaagaacatg
     2101 aacacaccca aacgatataa tccatttctt gtcacactgc actggcttac cgtggtgctg
     2161 atgctcgggg cggggctgct ttctgaaagc ggaggcgatt ctccgatcaa ttttcatatg
     2221 atgttggggg gcgccttgct ggttgtgacg ctcattcgca ttggggtgcg cttcgccacc
     2281 aaacaccccg ctcaggcaga tacgggcaat ccgctcttta atcagcttgg tcaatggatg
     2341 cattacggtc tatatcttgc gctatttttc atccttggca tgggcgcctt gatcgcaaac
     2401 aagaggaatt tgctggctgt ggcaatgggc accgggtcgg tcacaggcag ggctggtttt
     2461 ctcggtgata ttcaccatct cggctggttc cttgccattt tgctgatcct tggtcacgtg
     2521 ggtgcagcac tgtaccatca attcattctc aaagacaacc tgctgggcag gatgtggttt
     2581 ggaaagcaat aaagaaacag atgcaatttc atcaaaggat acaaaaaacg cggacacttc
     2641 tcaagtccgc gttttttgtg tcagtcagcg cttgcacgat caaaaatact gaaaggtcta
     2701 ttcccctttg tgaatatgcc gttgccgcaa gatcagcagg gataatccct gcacgaccag
     2761 ggtggcgaga atcgcagatc caaatgaagc tgcaaggctc gaccgctcga tcaacaagcc
     2821 aagcacaaga taaaaccctg caaacgaaaa caagccaagg atcaatccgt gcagcacatt
     2881 caccgcgcct gcatggcctt gatgccgatg tgaaaagatc gtcaaaatgc tgacgtacaa
     2941 tgggatcgtg gtcagcagtc ccgtcaggcg tgagccgatg tagggggcaa tgcctgtgat
     3001 gagcaaaata aaactcgtgc cgatcaaaat cctcgcgggg atatcccatc cgcctgggtt
     3061 ggaaacgggc ggcacggctt gtccctttgg catcagccgc aagccgataa aaataacgac
     3121 gatcaccgca gcaaaaagcg ggagaaacgg gatcgtgata ttttgcagca aggtggtcat
     3181 gcttgcaaac acgaccacac ccgacaaaat ggaaacgacc caattaaact tccgcgaaac
     3241 ccaggcgtag gtgaggctga atgcaacgag ggaaaagccg ccgctcaacg taccgcggac
     3301 tgaatcgaat acaaaggcac ggtcgtggct gagggcgagg aaaaaagcca ccggccccga
     3361 ggtaagcgga agccccacca gccatccgct cacggccgcc ccccaccgac gcccagccat
     3421 acttgccgag ccaatgatga ccggggcgag gatcaatttc aacatcaaaa tattcatggg
     3481 actgccatcc aatagatcgt taccagaatt gtaaaatcgg cggccatgtg ccccaacgaa
     3541 gcagaaagca aaccgccatc ttcacgggag atctgccgcc ataaccagcc cgccgaaacc
     3601 agaacggaaa ctgcaaacaa aataaagacc agatgcaccc tgccccacag gatcatcgca
     3661 tgataacccg caaaaacgac atcgccgaaa tacggtttga gcgagtcatt gcccaacaca
     3721 ccgcgccaga aatattcttc aacgaatgga ttgaccagtg aaaaataagc caggaaccag
     3781 ggccatgatg cgggcgtcaa tcccatcgcc aaaagttgcg cggagagatc tccaatcacg
     3841 ccaaacacag gccagagaaa atacagtcca attccgctgg tgctgcaaaa aaggacactg
     3901 gctagaatcc attttggcga gcggcttttg aaaagcgtgc gtacagacag gctgggacgc
     3961 aaaaccaaaa ctgtcaacaa gatcgcgatg tgaaacccga acagggctgc ccatgcattg
     4021 ttgaaaaaaa acaaccccac tcccacggca aggtagggaa aaatgggggc aaatattttg
     4081 aattgattca tattgaaaaa agtcaacccc gttgtgtcgt tatttgatct cggtgataac
     4141 cgttcgcttt gcacctgcag atattaaagc cgccgctaca gactcggccc tggcagggtc
     4201 aaccaacgca atcatattgc ccccgcgtcc gccgccgctc attttggcgc cgagtgcccc
     4261 agccttgcgc gccgcctcca ccaaagaatc caattcagga gaagagactg tcagctgttg
     4321 tagatattca tggttgtgat ccatcagctc gccgagcatt tcggttcgtc cgctttcgat
     4381 ggaacgcctg ccgatcaatg cgatctgcgc gatctcgttg aaaatgtttt ccaccgtaaa
     4441 ctggtcgcgg atccacagtc ggcgcacatc gcccaccgat tcttttgtgg gcgcggcaat
     4501 gcctgtatca ccgatgacga tggtgaacgg cctgccaacc ttgaagatct ccacaggctt
     4561 gtctttcata aaataaacgg gtttgccgta agtgatgacg gtgttatcaa tgcctgaggg
     4621 tgtgccgtga tggatctttt cgatctcata ggcaagtgaa ttcacttctt catctgaaaa
     4681 tggctgggag aggaaagcag aaagagcacg gatcaacgca accgacaccg ccgccccaga
     4741 ccccagcccc gccgcaacag ggatggtgga tgagatattc acttcgagat tcggcagcgg
     4801 ggaaatgcca aagtgctgaa tcactttcag gatcaccgaa gcaattgcat ggtcaggcgg
     4861 aagcgaattc aattcggcgt gcaggtcaat gatcggggcg tgaatccaaa tccctgtgcg
     4921 gggcgaatcc aaaacctcga catccacatg aacctgagtg acgggcaccg ccagtgcagg
     4981 atgtccaaac accgctgcat gttcgccgaa aagaatgatc ttgccgtgtg cggaggcttt
     5041 catgaaaggt aaaagatcgc caatacggtc agtccccaaa gaaccatcgc aagttgaaat
     5101 ggacggtctg aaagcagcac ttcttcgggt gcgccgcctg catgtttcac ttcaatgaga
     5161 tacatgtagc ggaagatggc atacaccaca aagggaatgg tcagcatcat cgtgtgattc
     5221 tcgggcaggt tgggcgcgga gaaagtgtac agcgagtaag ccacaatggt ggcccccgaa
     5281 acgatcatga tgtactggtc aagcaaaggc agggtgtaac catcgagcac tttgcggtgt
     5341 gagcccgctc catttgcaag caacgcaagt tcggcgcggc gtttgccaaa accgaggaag
     5401 agcgaaagca gagtcatcac cacgtaaagc cagggcgaaa atctttccac ttcaatgagg
     5461 gtaacacctg cacccacacg cagcacaaag cccgttgaaa taatcaacac gtcgagaatc
     5521 ggaacgtgct tgagccactt cgagtaagca aggttgatga cgaaatatcc gccgatcacc
     5581 gcgagcaaag acggcgtgag caggtatgcc agcacaaaag tgatggccac aaacagaaca
     5641 gcggcagtga ccgccacgcc cacaggcagt ttacccgagg caatggggcg gttcttcttt
     5701 tcagggtgct ggcg
//
LOCUS       DAOQLC010000198         5703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6214_length_5703_cov_25.287181, whole genome shotgun sequence.
ACCESSION   DAOQLC010000198 DAOQLC010000000
VERSION     DAOQLC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6214_length_5703_cov_25.287181"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1051)
                     /locus_tag="PL000_20045"
     CDS             complement(131..1051)
                     /locus_tag="PL000_20045"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNC91228.1"
                     /translation="MKAIVANQYGGPEVLQLKEVGKPQPKDNEVLIKVHATTVTMGDF
                     RMRSFTVPPVFWLPARLTLGITKPKQPIYGMELAGRVESIGKNVTRFKIGDQVFASTL
                     TENFGAYAEYKCLPEKAMMAIKPANISFEEAAALPIGATTALRLLRKGKIQRGQKVMI
                     YGASGSVGTYAIQLAKHLGAEVTGVCSTANLEMVKSLGADHVIDYTKETLSATKERYD
                     VIFDTVGKFAKSKASQILMPNGAFITMAQLDSKESMDNLIFIKELVEAGKLKVVIDRC
                     YPLEDMVEAHRYVDSGRKKGNVVITVGNTK"
     gene            complement(1099..1788)
                     /locus_tag="PL000_20050"
     CDS             complement(1099..1788)
                     /locus_tag="PL000_20050"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HNC91229.1"
                     /translation="MKAIIQENTQIKIPARKAQTSWWVPAGLILLSVIPLTFGAIRLS
                     QLINGAEITPANARFFAAPLPVVIHIISSAIYAVLGAFQFVSRLWQRGIKWHRWVGRL
                     LVPFGLFVGFSGLWMTLFYPRPTNASDLLYALRLFFGSGMILSIIIGFLSIRRRNVAE
                     HQAWMTRAYAIGMGAGTQVLTGIVGALVIGEVNEFENALLMGAAWVINLAIAEWNIRR
                     DATRQIRTTQV"
     gene            complement(1863..2594)
                     /locus_tag="PL000_20055"
     CDS             complement(1863..2594)
                     /locus_tag="PL000_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91230.1"
                     /translation="MKTNGKTSIKESKMKMTTSTLMRLAGVSAVLAGLCFIIIGMFHP
                     ENVTSSVTTTTWVNVHIAATALGFFGLFGLAGLYARQVEKSGWLGLTGFLLFSIWFVL
                     VSGFSFVEAFILPQLAAESPAFVESLLGMFTNTPTTIDLGVLPTLWNVSGPMYILGAL
                     LFGIATFRAQVLPRWAAGLLTLGAVLIPIGGMVPHEYQAKIAMIPIGLAMTWLGSALI
                     FERRMKASESLISQRISTPEASKVA"
     gene            complement(2767..5493)
                     /locus_tag="PL000_20060"
     CDS             complement(2767..5493)
                     /locus_tag="PL000_20060"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC91231.1"
                     /translation="MISPILATKLYSPPPRPGTVTRLRLIERLDEGLRSGHKLSLISA
                     SAGFGKTTLVGEWITKSGLPVAWLSLDDGDNDPVRFISYLIAALQTIKAEIGESLLAA
                     IQAHQPPSNESILTALLNEITVHLKEFLFVLDDYHTIDSKPVDEILLFLIEHQPPQMH
                     LVITSREDPALPLARLRVRGQLTELRAADLRFTPTEAAEFLDHAMNLNLTADEVTALE
                     SRTEGWIAGLQLAALSMKGQEDVHGFIQAFAGDHRYIVDYLVEEVLQHQPEQIRDFLL
                     QTSILERLNGPLCDVLTAQSGSKLKLEQLQRGNLFLIPLDDKREWYRYHHLFADVLRM
                     YLTTEQPDLVSVLHLRASEWYEQNNLTAEAIRHAVAGENFERAAGLIEKTLPLMRQSR
                     QESAMLGWFKALPEELLRKHPVLNVNYAGTLLQVGQFDGVEARLQDVEQWLATPEDIR
                     ERPVYVSEEEFQRLPSSVAMYHAAIALAQGDVADSIKYANKVLELAQTDKDFPRGAAS
                     SLLGLASWTGGDLETAYRLFADGMDHLLKVGFISDVVGGSITLADIRITQGRLHEAMS
                     IYERGLQLATKHEVPILRGAADMYVGMSELHREWNDLLAAEQYLLKSRELGELNGLPK
                     NPYRRRFAMARIHESRGNFEEALELLNEAEPLYIGDFSPNVRPIQALKVRVWIKQGEL
                     EKALAWARERELSSTESPSYLREFEQITFARILLLQYESDAADSLLHGVTQLLENLLK
                     AAESGGRMGSVIEILVLQALAYQMQADYASALKSLERALKLAESEGYIRIFVDEGAGM
                     AGLLRKALAKGIMPDYIGKLLTAFKVADQGLGVEMSPSAVPVSSSMIEALSQRELDIL
                     RLFKTELSGPEIAQELVIALSTVRTHTKSIYNKLNVNSRRGAVKRAIELGLI"
BASE COUNT         1432 a         1414 c         1370 g         1487 t
ORIGIN      
        1 gtgccatcat tttcctgggt gatgcttaat ccattgaatc gatctttcca ctgctgacct
       61 aggtgacctt ttactctgat ttgatagatc cttggttcat ccaggtcagg ttttgatatt
      121 tgcccaaggg tcattttgtg ttccccactg tgatgacgac atttcccttt ttacgtcctg
      181 agtcgacata gcgatgtgcc tcgaccatat cttccagcgg ataacagcgg tcaatgacta
      241 ctttgagttt tcccgcttcg accagttcct tgatgaagat cagattatcc atgctctctt
      301 ttgagtccag ttgtgccatt gttataaaag ccccgtttgg cataaggatc tgcgaggcct
      361 ttgatttcgc gaattttccc accgtgtcga agatgacgtc gtagcgctct ttagttgctg
      421 aaagagtctc tttggtgtaa tcaattacat ggtcagcgcc cagagatttc accatctcca
      481 gatttgcggt gctgcaaacc ccggtcactt ctgctccgag atgctttgcc agctgaatgg
      541 cataagtgcc aacacttccc gaagcaccat agatcatgac cttttgtccg cgctggatct
      601 ttccttttcg aagcagacgc aatgcagtag ttgcgccgat cggaagggcc gcggcttctt
      661 caaagctgat attggctggt ttaatggcca tcatcgcttt ttctggcaga catttatatt
      721 cggcgtaagc gccaaagttt tcggtgaggg tcgaagcgaa gacctgatca ccgattttga
      781 atcgagttac atttttaccg atagattcca cccgaccggc aagttccatg ccgtagatcg
      841 gctgtttggg tttggtgata cccagcgtaa ggcgggcagg gagccagaac acgggcggga
      901 cggtaaaact ccgcattcta aaatcgccca tggtgacggt ggtcgcatgc actttgatca
      961 agacctcgtt atcttttggc tgtggtttcc caacctcttt gagttgaaga acttcgggac
     1021 caccatattg atttgctacg attgctttca ttttagactc cttgttttat aaatactcag
     1081 taaacctgat gtcgatattc aaacctgtgt ggtgcggatc tgccttgtgg catccctgcg
     1141 aatattccat tcggctatcg caagattgat cacccaggct gcgcccatta acaatgcgtt
     1201 ttcaaactca ttcacttcgc caatgaccag ggccccgact atcccggtca acacttgtgt
     1261 gcctgctccc ataccgatgg cataggcacg tgtcatccag gcttggtgct cagcaacatt
     1321 ccgccgtctg atcgagagga atccaatgat gatggataga atcatgccag agccaaaaaa
     1381 gaggcgcagt gcataaagca ggtcgctggc attggttggg cggggataga ataaggtcat
     1441 ccaaagtccg gagaatccaa caaacaaacc gaatggaacc aaaagccgcc cgacccaacg
     1501 atgccatttg atcccgcgtt gccagagtcg gcttacgaat tggaatgctc ccaatacggc
     1561 atagatcgct gagctgataa tatgaatgac aacaggcaaa ggggcggcaa agaagcgggc
     1621 attggcaggg gtgatttctg caccgtttat caactggctt agacggatgg ctccgaacgt
     1681 cagcggaatc acactgagta agatcaaccc agcgggtacc caccatgatg tctgagcttt
     1741 tctggctggg atcttgattt gagtattttc ctggattatt gctttcatca ttaactcctt
     1801 gaaattattt atgaactttt agagacaaac ggttaggcaa ccgcttgtct ctatgattaa
     1861 tctcaagcaa ctttgcttgc ctctggtgtg ctgattcttt gggagatcaa ggattcagat
     1921 gctttcatac ggcgttcaaa aatgagcgcg gatcccagcc aagtcatggc aagcccgatc
     1981 ggtatcattg cgatctttgc ctgatattcg tggggaacca ttcctccgat ggggatcaac
     2041 acagcaccaa gggtaagcaa gcctgctgcc cagcgaggta aaacttgggc acggaaggtg
     2101 gcgatgccaa acaacaaggc acccaggata tacatgggcc ctgaaacatt ccacaaggtt
     2161 ggcaggactc caaggtcaat tgtggtggga gtgttggtaa acattccgag aaggctttcc
     2221 acaaatgcag gcgattcggc tgccaactgc ggcaggatga aagcctcgac gaacgaaaaa
     2281 cctgatacga gaacaaacca gatgctaaat aggagaaagc cggtcaaccc cagccagccc
     2341 gatttttcca cctgccgtgc atagagccca gccaagccaa acaatccgaa gaagcccaaa
     2401 gcggtcgcag caatgtggac attcacccat gtggttgttg taactgatga agtgacattc
     2461 tccggatgga acattcctat aatgatgaag cacagccctg ccaatacagc agatacccct
     2521 gccagccgca tgagggttga agtagtcatt ttcattttgc tttctttgat cgaggtcttt
     2581 ccattggttt tcattggtag tctcctttta agtttttggc gttgccgcct gttgagttcg
     2641 attgaagaca atatacaaat taacatgggg agatgtcttc cccacatgtg gggatttcat
     2701 ggggtgagaa tcgaaaaccc cgccagaacc atgtggcggg gtttggaaat ctgtttggta
     2761 gagagcttat atcaaaccaa gttcaattgc ccgtttgaca gcccctcgtc ggctgttcac
     2821 gttgagtttg ttgtagatgc tcttggtgtg tgtgcgaacc gtgctcaatg cgatgacaag
     2881 ttcctgggcg atctcggggc ctgataactc ggtcttgaac agacgcagga tatccaactc
     2941 acgctgacta agcgcttcga tcatggacga tgaaactggt acggcagaag gagacatttc
     3001 cacccccaaa ccttgatctg ccactttaaa tgcagttaga agtttgccga tgtaatctgg
     3061 catgattcct tttgcgaggg ctttgcggag caaacctgcc atccctgcac cttcatccac
     3121 aaatatgcgg atataaccct ccgactcagc cagtttgaga gcgcgttcaa gcgatttaag
     3181 agcggaggca taatccgctt gcatttgata agccaatgcc tgcaagacca gaatctcgat
     3241 cacacttccc atcctgccac ctgattccgc tgccttcaga agattctcca gtaattgtgt
     3301 tacaccatgc agcaaactat ctgcagcatc gctttcatat tgcaaaagca aaattcttgc
     3361 aaaagtgatt tgctcaaatt ccctcaggta actgggcgat tccgtactgg ataactcccg
     3421 ttcgcgcgcc caggcaagag ccttttccaa ctcgccctgc ttgatccaaa ctcgcacctt
     3481 caatgcctga atgggccgaa catttgggga gaaatcacct atatacagag gttctgcttc
     3541 attgagtagt tccagtgcct cttcaaaatt gccgcggctt tcatggattc gtgccatggc
     3601 aaaacgcctg cgataaggat ttttgggcaa tccattaagt tcgcccagtt ccctgctttt
     3661 caaaagatat tgttcggcgg cgagcaaatc gttccactca cgatggagtt cgctcatccc
     3721 cacgtacatg tctgcggcac cgcgaagaat gggcacttca tgttttgttg caagctgtaa
     3781 tccccgctcg tagatgctca tcgcctcatg caaacgaccc tgtgtgatcc gtatatcggc
     3841 cagagtgatg gaaccgccga ctacgtcgga gatgaaccca accttcagta aatggtccat
     3901 gccatcggcg aataaccggt aagcggtttc aagatcgcca cctgtccacg atgcaagtcc
     3961 cagcaatgat gatgcggctc cgcgggggaa atccttatcc gtttgggcga gttcaagcac
     4021 tttgtttgcg tattttatgc tgtctgccac atcaccttga gccagggcaa tggcggcatg
     4081 gtacatggcg acagaactag gcagacgttg aaactcctct tcacttacat acacaggccg
     4141 ttctcgaata tcttccgggg tagccagcca ctgttccaca tcttgcaagc gtgcctcaac
     4201 accatcaaat tgaccaacct gcaacaatgt acccgcatag ttaacgttca gcacgggatg
     4261 tttccgtagc aattcttcag gaagtgcctt aaaccaaccc agcatcgcgg attcctgtct
     4321 gctttggcgc atgagcggca gcgtcttttc gatcagcccc gcagcccgct caaaattctc
     4381 tccagccact gcatgacgga tcgcttctgc cgtcaaatta ttttgctcat accattcact
     4441 tgcacgaagg tgcaagacag aaaccagatc aggctgttct gttgtcaaat acatccgaag
     4501 tacgtcagca aaaaggtggt gatagcgata ccattcacgc ttgtcatcca atggaataag
     4561 aaacaaattg cctcgctgca attgttccag tttcaatttg cttcccgatt gggcagtgag
     4621 gacgtcacaa agcgggccgt tcaagcgttc gaggatggat gtttgtaaca aaaaatcccg
     4681 aatttgctcg ggctggtgtt gtaaaacctc ttcgaccaga taatccacaa tgtagcggtg
     4741 gtctccggcg aatgcttgaa taaatccatg gacgtcttcc tggcctttca ttgaaagtgc
     4801 agcgagttgc agaccagcga tccaaccttc ggtacgggac tccaatgcag taacttcatc
     4861 tgcggtgagg ttgaggttca ttgcatgatc gagaaattca gctgcttcgg ttggagtgaa
     4921 gcgcaaatcc gcggcgcgta attctgtgag ctgtccacgg acacgcaggc gagccagggg
     4981 cagggcaggg tcttcgcgcg aagtaatcac caaatgcatc tgcggtggct ggtgttcgat
     5041 cagaaataaa aggatttcat caaccggctt ggaatcgatc gtgtggtagt catccaaaac
     5101 aaaaaggaat tctttcaaat ggacagtgat ttcattgagc aacgctgtca aaatagattc
     5161 attggaaggc ggttgatggg cttggatcgc tgccaataaa ctttcgccta tttccgcctt
     5221 gatggtttgt aaggccgcta tcagatagga aatgaaacgg accgggtcat tatccccatc
     5281 atccaacgat agccatgcaa ccggtaggcc gctcttggtg atccattcac cgaccaatgt
     5341 cgttttgccg aacccagcgg aagcagaaat aagacttaac ttgtgacccg aacggagccc
     5401 ttcatccagt cgttcaatca ggcgcaggcg tgtaacagta ccggggcgtg gtgggggact
     5461 gtatagtttt gtcgccaaaa ttggtgagat cataaattca ttatataaca agcataaata
     5521 aaacatgatg gtttgtagcc atacagacag gcttcaatat gttgggtctt ttaaatctcc
     5581 aaaaagttcc gtacgtaggt cttgaattta tacctgcgtt aagttgggta agttttatgc
     5641 aagcttggac aaacaatttt cattccgatt gttgacaaaa aacaccctgt tggagtatat
     5701 tgt
//
LOCUS       DAOQLC010000199         5695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6229_length_5695_cov_16.246809, whole genome shotgun sequence.
ACCESSION   DAOQLC010000199 DAOQLC010000000
VERSION     DAOQLC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6229_length_5695_cov_16.246809"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PL000_20065"
     CDS             <1..188
                     /locus_tag="PL000_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91232.1"
                     /translation="DNKNYVSSPIRKAGKVYCIGVLEPCLFTLSAPNEQKQFWGSLKP
                     LDLGCLEIYQSSNLPAN"
     gene            complement(515..1114)
                     /locus_tag="PL000_20070"
     CDS             complement(515..1114)
                     /locus_tag="PL000_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC91233.1"
                     /translation="MRIPENKKMLIGYARVSTEEQNLQLQTDALTKVGCERIFTDKAT
                     GSNLNRPGLTEALSHLRPGDTLVIWKLDRLGRTVKGLVDFVTKLEAQDVNFHSITDKI
                     DTSSTAGRFFFHIMASLSQMEGELTRERTKAGLAAARAVGRVGGRQRRMTDNKIEAAK
                     KLLISGKTPRDVAKILEVSVPTLYRWIPEAASLLINEPS"
     gene            1247..4279
                     /locus_tag="PL000_20075"
     CDS             1247..4279
                     /locus_tag="PL000_20075"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNC91234.1"
                     /translation="MPNISDTAYPRPKARPSQKELERNYTPTEEERAFAWQRTSTEIQ
                     TLRLLVWLKVFQHLGYFPSHDEISSSIIEHIAQWVDVEEPSAALSSYDGSNQKWVHHG
                     MVREYLCVSTFNKSARQIAVAACLEAAATRDDLVDIINVAIEELVRQRYELPGYSTLA
                     KIARAARSRVNTDYHQQVYSALTSKTKDGLKLLFESPGRQSKSAWDNLKKEPNKPTVR
                     NLQDFLRHLSMLLEHDLRSEAFGQIADVKVNQFAAEARSLDVASMRDFAEPKRLTLAA
                     ALVLVQVGRAYDDVADMFIRLVQRMHAKAKEALRIYREEKAAETDELVNTLRAISVAY
                     KADDTELNRLAAIGLVFGDRVDEIIERCEKHATLAGNNYLPLLPSFYKGQRPVLLSFL
                     ENVDLLSTSQDQSILSAIKFILQNKTSRAEWLTAPSDLDLSFVSDKWMPLVTGNKNPA
                     ALFDKVHRRNLELCIFTEVMNELKSGDLCIPRGDKYRDYREQLISWEEFYRDIALYGE
                     QSGIQTEPKLLVATLQSQLQAVAQKADEGFPSNEYLSIENGEPLLKRLTAAPIPPGLQ
                     SFEEYLKQYMKQNDVLNVLWQTEQWLNWTKHFGPISGLDSKIENPQERYVINTFCYGC
                     NLGPTQTARSISLVDRRQISFINQRHVTEQKLDEAIVSVINAYKDFPLQRLWGLGKTG
                     SADGTKWDLYPQNLMSEYHIRYGGYGGIGYYLVADSYIALFSRFTTCGAWEGHYILDF
                     VTENDSEVQPDTIHSDTQGQSAPIFGLAFLLGIKLMPRIRNWKDLKFYRPSSDFRYEH
                     LDSLFASEEIDWKLIETMLPDMLRVALSVKAGKITPSAILRRLNTYSRKNKLYFAFRE
                     LGNVIRTKFLLEYISDISVRRMISVATNKSERFNQFADFLLFGGKGVIAENVRDEQRK
                     IIKYNHLVANLVILHTLVTMSDALQAMADDGLYIDPSVVACLSPYQTEYINRFGNYKL
                     KKGKPATSLDPALKITLFDEFPAFQTVQHSSDGRPSHF"
     gene            complement(4633..5433)
                     /locus_tag="PL000_20080"
     CDS             complement(4633..5433)
                     /locus_tag="PL000_20080"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC91235.1"
                     /translation="MSALNANAVGNLESFQGAGTDYQPGTNDGTKLVPGQKFSPVGNT
                     ASETVAKHQPAPFQEGFSDFFEAAAAEVHPGSTVEPDQNQSGTEPEPVLNLKEAVEFY
                     KISEKTIRLYIKDGKIPARKEEGPRGLEWRIYPKGLPKDIPASETSALEPELVQTGSD
                     VSETEDNTIVEPERYQTGTTPEPSWYTDAKITGASQVPVQPSFAPELKSLLDVITKQA
                     EKLEAASMRIGYLEARTENYEKEIKLLTDSQHKRGWWARLGAWFIGKK"
BASE COUNT         1466 a         1385 c         1366 g         1478 t
ORIGIN      
        1 ctgataataa aaattatgtt tcatcgccga ttagaaaagc tggaaaggta tattgtatcg
       61 gggttttgga accttgttta tttactcttt ccgccccaaa tgagcaaaaa caattttggg
      121 gtagcctaaa accgcttgat ctcggatgtt tagagattta tcagtcatcc aatttgccag
      181 ccaactaact gtgaggattt actgtggacc atccgaaaat gatatgtgct caggagtttg
      241 tccttgtaga cgaggaaggc tcaccgagag ccacttgggc aattgatgat ggcgggcaag
      301 ctgcgatcag ctttttagac cgtgacggta atggcaagct gctcgttggt gtcggcaaag
      361 acgggctagc cgtaatcact ctaaaggacc aacggtaagg atcctggaca aaaaccctat
      421 cgcttcttga aagccattcg caagtcaaat tttatgagcg tttcgccgcc gcactccgct
      481 tgtctaccgg caaaagtggc ttaaattcct ggagttatga aggctcattg attaaaagcg
      541 aggctgcttc aggaatccag cggtagagag ttggaactga tacctccaga atttttgcaa
      601 catctcttgg agtcttgccg ctgatgagga gtttcttcgc tgcctcaatc ttgttgtctg
      661 tcattcgtcg ctgccgtcca ccaaccctac cgacggctct agcggcagcc agaccggctt
      721 ttgttcgctc acgtgtcagc tccccttcca tttgcgagag actggccatt atgtgaaaga
      781 agaagcgacc agcagttgag ctggtgtcta tcttgtctgt aatgctgtgg aaattcacat
      841 cttgtgcttc cagcttggtg acaaagtcaa ccaagccctt aactgtgcgt cccaatctat
      901 ccagtttcca gatgaccaat gtatcgcctg ggcgcagatg agacagggct tctgtgaggc
      961 ctggccgatt caaatttgaa ccggtggctt tgtcagtgaa aattcgttcg catccaacct
     1021 tggtcagggc atcagtttgc agttgcaggt tttgctcttc ggttgaaacg cgggcgtagc
     1081 caatcagcat ttttttgttc tcaggaattc tcaaaactct tcttgattat gcgatagtac
     1141 tccaggagtt tcaagagtgg tttttgagaa cccaagaagc cccatttatg gcagacgttg
     1201 gccgggcatc ccaggattat tgcaaatgtt cgtttgtgag aagtgaatgc cgaatattag
     1261 cgacaccgca tatcctcgcc cgaaggctcg gccaagccag aaagagcttg agcgaaatta
     1321 tacgccaact gaagaagagc gtgcctttgc ctggcaacgg acatccacag agattcaaac
     1381 attgcggctg ctagtctggc tcaaggtctt tcagcatctt ggatattttc catctcacga
     1441 tgagatctca tcatcaatca tagagcacat tgcgcaatgg gtggacgtag aagagccatc
     1501 tgctgctctt tctagctatg atggcagcaa tcaaaagtgg gtgcaccatg gaatggtgcg
     1561 ggagtattta tgcgttagca cgtttaacaa gagtgcgaga cagatagcgg ttgctgcctg
     1621 tcttgaagct gctgcaacca gggacgattt ggttgatatt atcaacgtcg caattgagga
     1681 gctagttcgg cagcggtatg aattgccggg ctacagtaca ctagccaaaa tagcacgggc
     1741 agctcgttct cgcgttaaca cggactacca tcagcaggta tactcagcgc ttacttctaa
     1801 aactaaagac ggtttgaaac tgctttttga atcgccaggc agacaatcaa aatctgcctg
     1861 ggacaaccta aagaaggagc ccaacaaacc aactgtcagg aatctacaag atttcctgag
     1921 gcatttgagt atgcttctgg agcatgatct tcgaagtgaa gcatttggtc aaattgctga
     1981 tgtaaaagtc aaccaattcg ccgcagaagc tcgttctttg gatgtggctt ctatgcgaga
     2041 ctttgctgag ccaaagaggc tcactctggc tgcagcactc gtgctggttc aagtcggccg
     2101 agcttatgat gatgttgccg atatgttcat tcggctcgtc cagagaatgc atgccaaagc
     2161 caaagaagcg ctccggatct acagagaaga gaaagcggct gaaactgacg agctagtcaa
     2221 cacacttcgt gcgatatcag tggcgtataa agccgatgac actgagctaa accgcttagc
     2281 tgcaatcggt ctggttttcg gtgacagagt ggatgaaatt atcgagcggt gcgaaaagca
     2341 cgctactctt gccggcaaca attatttgcc actactgccc agcttttata aaggacaaag
     2401 accagtatta ctgagcttcc tggagaatgt agacctgctt tcgacgagtc aagatcagag
     2461 cattctgtca gccataaaat ttattcttca aaataagacc agtcgcgcag aatggttaac
     2521 tgcaccatct gaccttgatc tctccttcgt ttcagataag tggatgccac ttgtcacggg
     2581 caataaaaat ccagcagcgc tgtttgacaa agtgcacagg cgcaatctcg aactctgtat
     2641 atttaccgaa gtgatgaatg aattgaagtc tggtgacctc tgtattcctc gcggagacaa
     2701 gtaccgagac tatagagaac aactcatctc ctgggaggaa ttttacagag atatcgcgct
     2761 ttacggcgaa cagtctggca tacagactga gccaaaacta cttgtggcga cgttgcagag
     2821 tcagcttcaa gcagtagcac aaaaggccga cgaaggtttt ccatcaaatg aatacctcag
     2881 tattgagaac ggagagccgc ttttgaagcg acttacagca gctccaattc ctccagggtt
     2941 gcagtcgttt gaagaatatt tgaagcaata catgaaacaa aatgatgttc taaatgtact
     3001 gtggcagact gagcaatggc tgaactggac caagcatttc ggtcccatat ccggactgga
     3061 ttcgaaaatt gagaatcccc aagagcgtta tgtgattaat acattttgct atggatgtaa
     3121 tcttggaccc acacagacag cccgttctat aagcctcgtc gacaggcggc agatttcttt
     3181 catcaaccag agacacgtga cagagcagaa actggatgaa gccattgtgt cagtcatcaa
     3241 tgcctataag gactttcccc tgcagaggtt atggggactt ggcaaaactg gctcagccga
     3301 tggcacgaaa tgggatcttt acccccagaa cctaatgtcg gaataccaca tccggtatgg
     3361 tggatacggc ggcattggtt actatctggt agccgacagc tacattgcgc tcttctctcg
     3421 attcactacc tgcggtgcat gggagggtca ctatattctg gactttgtca ccgagaacga
     3481 ttctgaggtc cagcccgata caatccattc agacacgcag gggcagagtg ctccgatatt
     3541 cgggctggca ttcttgcttg gcattaagtt aatgccgaga atcaggaact ggaaggatct
     3601 gaaattttat cgcccaagct cagactttag gtatgagcat ttggattccc ttttcgcgtc
     3661 ggaagaaatt gattggaagc tgatagagac catgctgcca gacatgctca gggtagccct
     3721 atcagtcaag gctggcaaaa tcaccccttc cgcaatcctt cggcgcctca acacctatag
     3781 tcgtaaaaac aagctctact ttgcctttag agaacttggc aatgtcatca gaacaaagtt
     3841 tttactcgaa tacatttcgg atatctctgt ccggcgcatg atctccgtcg ccacaaacaa
     3901 gagcgagcga ttcaatcaat tcgccgactt cctgctcttc ggcggcaaag gcgtgatagc
     3961 tgaaaatgtc agggacgagc agcgtaaaat catcaagtac aatcacttgg tcgcaaacct
     4021 ggtcatactg cacactttag tcaccatgag cgatgccttg caggccatgg cagacgacgg
     4081 gctatatatt gatccaagcg tggttgcctg ccttagccct taccagaccg aatacatcaa
     4141 tcgttttgga aactacaaac tcaaaaaagg aaagcccgct acatctcttg atccagccct
     4201 aaaaatcacg ctgttcgatg aatttccggc atttcagaca gtacagcact cctcagatgg
     4261 cagaccaagc cacttctaaa agatcacctg ccctgccaag tgcaacagtc ccggtaaagg
     4321 cttgcctgta catgcgtgcg aaatgttttc aagcagcagt agggtttttg tccaggatcc
     4381 ttaccgtcgg tctaaaggcc gcaaaacagt ttcctgcaac ccttataact gttgatcagg
     4441 ctggcgattt caaatttgcc tgcctggatt ctgacgggca aaaccgcttt caactaacag
     4501 tcgctgctga tggctcatcc gtacttatgg gactgggcgg caatcctccg aatgtcctac
     4561 tgagcgctat caatggtgaa ggcgaaattg tcagcaaccc gattagcaaa aggtagaaca
     4621 acagttagaa tactactttt taccaatgaa ccaagcacct aaacgagccc accaaccacg
     4681 tttatgttga ctgtcggtca acagttttat ctctttctcg tagttttcgg tcctagcttc
     4741 aaggtatcca atccgcatgc ttgcggcttc tagcttctcg gcttgctttg taataacgtc
     4801 cagaaggctt ttcaattctg gggcaaagct cggctgaacc ggcacttgag aagccccggt
     4861 gatctttgca tccgtgtacc aggatggttc cggcgtggta ccggtctggt accgctctgg
     4921 ttccacgata gtattgtctt ctgtttcgct gacgtcggaa ccagtctgaa ctaactccgg
     4981 ttccagggcg gaagtctcag aggctgggat atccttcggg agccctttgg gatatatccg
     5041 ccactctaaa ccccttggcc cctcttcttt ccgtgcggga atctttccgt ccttgatata
     5101 aagtcggata gttttttcag aaattttata aaactctaca gcctctttta ggttcaagac
     5161 tggttccggc tcggtaccgg actggttctg gtctggttcc actgtggaac cagggtgaac
     5221 ctcggccgcc gctgcttcaa agaagtctga aaacccttcc tggaatgggg ctggttggtg
     5281 cttggcgacc gtctcgcttg cagtgtttcc taccgggctg aacttctgcc ctggtaccag
     5341 cttggtaccg tcattggttc caggctggta atcagtccca gcaccctgga agctctctaa
     5401 attcccaact gcattggcgt ttagtgcgct catttagatt tccggccttt cagggtggta
     5461 ccagtctcaa tcaagtgcat tatgcgtgga accgggctgg ttgtccagaa aattatatac
     5521 accgcatatc tcgaaacccg cactggttcc ttgatccccg aatcgatccg gcgtaacatc
     5581 tgagactgaa tagaactatt tgccactgcc tgtggcgtct ggagcaaaca gtactaaaga
     5641 aaaaaacacc gcctggggcg atgctttcta agacctataa ccggatcttt cggtt
//
LOCUS       DAOQLC010000200         5694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6236_length_5694_cov_17.921085, whole genome shotgun sequence.
ACCESSION   DAOQLC010000200 DAOQLC010000000
VERSION     DAOQLC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_5694_cov_17.921085"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..387
                     /locus_tag="PL000_20085"
     CDS             52..387
                     /locus_tag="PL000_20085"
                     /inference="COORDINATES: protein motif:HMM:NF039553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5856 family protein"
                     /protein_id="HNC91236.1"
                     /translation="MRLFHARTAAHVLHLQSRRYSEHMALNTFYEEIVGLTDDLAEAY
                     QGGYELIREYPSKYTPYSNALSLMDDLRDCIDAHKGDWDEEDTHLNNICDEIRQLIAS
                     TQYKLRFLS"
     gene            396..>1864
                     /locus_tag="PL000_20090"
     CDS             396..>1864
                     /locus_tag="PL000_20090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91237.1"
                     /translation="MPINDQLASEQWIRFQYCRDRGHLDFINKADKCEKFFKGEQWSV
                     TDLNTLHLQRRPALTLNKIISTLGTLFGEQIYNRNETIFRPASGAREETAEILCKVWK
                     QFSQRNQLPWTRSELFADGVIRSRGFVDMRLDFTDSMLGEIVLTNVNSKNVVVDPDAD
                     EYDPDSWNDVFTTKWITPQDVSILYNEDDAEYLKNLDGSAFPYGYDSIERVRDRFGGV
                     LPLAGYYGVTEPYGLRRNVRVLDRQYRKLDKQLHFVDVSTGDMRPIPNAWDRNRIATV
                     IEKAQGRVSTTKKLVKRIRWTVTADNVVLHDDWSPYKHFTLIPYFPYFRYGATIGLVE
                     NLLGPQELLNKVSSQELHVVNTTANSGWKLKAGALKNMSIEELEQKGATTGLVIELDD
                     IANAEKILPNATPQGLDRISDKAENHIKSISNISDSMQGFDREDVAAKAIQAKASRGS
                     INMTKVMDNLERTDYIIARNWIDLVQEYMTEPRIMHITG"
     assembly_gap    1865..1964
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1965..2393
                     /locus_tag="PL000_20095"
     CDS             <1965..2393
                     /locus_tag="PL000_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91238.1"
                     /translation="RMQQAEVTEKEAQAQQKQADAQLKMAKTKAELASIELEASKGDG
                     AEVIQMELQLEAEKAQQEQALEREKAEDKMSIEERKFQHELSMKEREMAMREEEHRQD
                     AQLKLDLHEHTKKIATQQAESQALAAQTNQINAQRSQSKE"
     gene            2398..3372
                     /locus_tag="PL000_20100"
     CDS             2398..3372
                     /locus_tag="PL000_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91239.1"
                     /translation="MTLYQKLFGLKLMAPADEDGGGGGGAPDRGDKFVPTGAGEEPES
                     PDAKVDEDETVIDEVPTPRTPDGKFAKKEKSPDDEPRIPKSRFDNAISKERARAEAAE
                     RRLAEIEAAQSQFQKNADISQLEGKLRELRAQEHKSVLLGDTDKAAQLADEADRLNRH
                     IAIAQSKDMSAADKGQALEQMRMELTLERIFEQYPFLDENSEDFDQDLVDDINDKQAG
                     LIGRKGMSPSKALVEAVRHIMAQRESSAPATKGKALGAPVLGRKEAAVAKNLDAARRT
                     PASTRVVGADSDKHGQTGPLPSVDDMTFEEFNALPEKTKARLRGDFVN"
     gene            3401..3796
                     /locus_tag="PL000_20105"
     CDS             3401..3796
                     /locus_tag="PL000_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91240.1"
                     /translation="MRKITDHVVNPANDRLTITVVDDPGHGGANHRYEISGFNAKSNS
                     SAGEDDNQRAVILFQNGPIDVDGNGVNGLTHEALLAIVADRLRSFQRGPYSCKANACA
                     LTHIEEAQHWLQQRTLERMRRGVEGTHAI"
     assembly_gap    4397..4496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4694..>5694
                     /locus_tag="PL000_20110"
     CDS             4694..>5694
                     /locus_tag="PL000_20110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HNC91241.1"
                     /translation="MSYTNFAQLTSEQKTTWSRDLWAQARNMSFVNQFLGKDSNSMVQ
                     HITELKKSEKGARAVITLLTDLEGDGIAGDRTLEGNEEAMKSFDQVIRLDQLRHANRH
                     EGRMADQKSVVSFREESRDKLAYWLSDRIDQLAFLTLAGIGYTQRNAGGTRVGSDLQY
                     LEFAADVTTPSSKRYARWDGASNKVLSWGTGSSAVTAADTPTWGMFVQAKAYAKDNYI
                     RGIKSKGGEEVYHAFLSPTAMAKLKLDDTYLQNLRYAVTRGDDNNLFTGTSVKIDGIV
                     LHEFRHVPNTRLAASGSKFGASGTIDGCQILFCGAQALGMADIGNPEWVEKGFDYEN"
BASE COUNT         1458 a         1360 c         1517 g         1159 t
ORIGIN      
        1 atcaccctgg tttggacgcc gatctgactc gtggctatcg gcgacctcat catgcggttg
       61 ttccatgctc gtacggcagc gcacgtacta catctgcagt cgcgcaggta ctccgaacac
      121 atggcgctca ataccttcta cgaagagatt gttggactga ccgatgacct cgcagaggcg
      181 taccagggtg ggtatgagct gattcgcgag tacccatcaa aatatacccc gtattctaat
      241 gctctctcac tgatggatga tttgagagat tgcatcgatg cgcacaaggg cgattgggac
      301 gaagaggaca cgcacctgaa caacatctgt gatgagattc gtcagttgat cgcatcgact
      361 cagtacaaac tgcgtttttt gagctagggg cgagtatgcc aataaatgac caactggcct
      421 cggagcaatg gatacgtttt cagtactgtc gtgatcgtgg acatctcgac tttataaata
      481 aagcggacaa gtgcgaaaaa ttcttcaaag gtgagcagtg gagcgtcaca gacctaaaca
      541 cgctccatct tcagcgtcgc cctgctctca ccctgaacaa aattatctcg accctcggca
      601 cgctgtttgg agagcagatt tataatcgca acgaaacgat ttttcgtcca gcaagcggtg
      661 cgagggagga aacggcagaa attctgtgca aggtgtggaa gcagttcagt caacggaacc
      721 agttgccgtg gacacgtagt gagttgttcg ctgatggcgt gatccgtagt cgtggctttg
      781 tggacatgcg gctcgacttt accgactcca tgcttggtga gatcgttctg accaacgtga
      841 actcaaagaa tgtggttgtt gatcctgatg cagatgagta tgacccggat tcgtggaacg
      901 acgtgttcac gaccaagtgg atcactccgc aggacgtatc cattctatac aacgaagatg
      961 atgccgaata cctcaagaac ttggacggat cagcattccc ctacggttac gattctattg
     1021 agcgagtgcg agaccggttt ggtggggtct taccgcttgc gggttattac ggagttaccg
     1081 aaccgtacgg cctgcgacgc aacgtacgag tgctcgaccg tcagtatcgg aaactggaca
     1141 agcagttgca ttttgtggat gtatccacgg gggacatgcg ccccattcca aatgcctggg
     1201 atcgcaaccg aattgccacg gtcatcgaga aggcacaagg gcgggtctcg accacgaaaa
     1261 agttggttaa acggatacgt tggactgtta ctgctgacaa cgtcgtgctc cacgatgact
     1321 ggtcgccgta caaacacttc acccttatcc cgtatttccc atacttccgt tacggcgcaa
     1381 ccattgggtt ggtcgaaaac ctgcttggcc cgcaagagtt gctgaacaag gtcagttcgc
     1441 aagagctgca cgtggtcaac actacggcga attctggttg gaaattgaag gccggtgctc
     1501 tgaagaatat gagcattgag gagctggagc agaaaggggc aacgaccggc ttggtgattg
     1561 agttggacga tattgcaaac gccgagaaga ttcttcccaa tgcaactcca caggggttgg
     1621 atcggatcag cgacaaggct gagaatcata tcaagtcgat cagtaatatc agcgactcaa
     1681 tgcagggctt tgatagagag gatgttgctg cgaaggccat ccaagcaaag gctagtcggg
     1741 gtagcatcaa catgaccaaa gtcatggata acctcgagcg tactgactac atcattgcga
     1801 gaaactggat cgaccttgtt caggaataca tgaccgaacc tcggatcatg cacatcactg
     1861 gtgannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgtatg cagcaagcgg
     1981 aagtgactga gaaggaggcg caggctcagc agaagcaggc ggacgcacag cttaaaatgg
     2041 ccaagacaaa ggcggaacta gcgtcaattg aactcgaagc ctcaaaagga gacggcgcag
     2101 aagtgataca aatggaactg cagctagaag cggagaaggc tcaacaggag caagcgctcg
     2161 aacgtgagaa agctgaagac aaaatgtcca tcgaagagcg gaagttccaa catgagttgt
     2221 caatgaaaga acgagagatg gccatgcggg aggaggagca tcggcaagat gcacagctca
     2281 agttggacct ccatgagcat acgaagaaga tcgcaacaca acaggcagag tctcaggccc
     2341 tcgctgccca gaccaatcaa attaacgcac aacgttcaca atcgaaggag taattgaatg
     2401 accctttatc aaaaactgtt cggacttaaa ctcatggccc ctgccgacga agatggtggc
     2461 ggaggagggg gtgcgccgga cagaggagat aagtttgttc ctaccggagc gggcgaagag
     2521 ccagaatcac cagatgccaa ggtggacgag gacgaaacgg tcattgatga ggtacctaca
     2581 cctcgtaccc ctgatgggaa gtttgccaag aaggagaaat ctccagacga tgagcctcgg
     2641 attccgaaga gccgttttga caacgccatt tccaaggaac gcgctcgagc agaagctgca
     2701 gaacgtcgtt tggcagaaat tgaggcggcg caaagtcagt tccagaagaa cgcagacatt
     2761 tctcagttag agggcaaact gcgcgagctg cgtgcgcaag aacacaaatc ggtgttgctt
     2821 ggcgataccg acaaagctgc gcaactcgcc gacgaggctg atcgcctcaa tcgacatatt
     2881 gcaatcgctc aatcgaagga tatgtcggct gcagataaag ggcaggcgct tgagcagatg
     2941 cgcatggagc tgacactcga gcgtattttt gagcagtatc cgtttttgga tgagaatagt
     3001 gaggatttcg accaagacct tgtagacgac atcaacgaca aacaagcggg gttaattggt
     3061 cgaaagggta tgagtccgtc taaggcgctt gtagaggcag ttcgtcatat tatggcccag
     3121 cgtgaatcat cggcacctgc gacgaaaggt aaagccttgg gagcacctgt actagggcgt
     3181 aaagaagcgg cagtcgccaa gaacttggat gccgcgcggc gtactccggc aagcacacga
     3241 gttgttggag cggacagtga caaacatggt caaacagggc cactgccctc agttgacgac
     3301 atgactttcg aagaattcaa tgcgttgccg gaaaaaacga aagcgaggct gcgtggagat
     3361 ttcgtaaact aattaggttc actaactatg aggaaacaaa atgagaaaaa tcactgacca
     3421 cgtagtcaat cctgccaacg accgtttgac catcacggta gtggatgacc caggacacgg
     3481 tggtgccaac catcgctacg aaatatctgg attcaacgcc aagagcaatt ctagcgccgg
     3541 ggaggatgac aaccagaggg ctgtcatctt gtttcagaat ggacccatcg acgtcgacgg
     3601 aaacggggtc aatggtctta cccacgaggc gctgttggcc atcgtcgcag accgtctgcg
     3661 gagcttccag cgaggcccct attcgtgcaa ggcgaatgcc tgcgccctta ctcacatcga
     3721 agaagcgcaa cattggttgc agcaaagaac gctggagaga atgcgcagag gggtggaagg
     3781 aactcacgcg atatgatcga cagcctatgg gtcactgctc aagatcctca tttacacccc
     3841 caagctcccc agctccctaa gtaaacatct tctcctacaa aaccccggcc ccccgaaact
     3901 gtccatacat aaacacctat acatcataga cttttcgtaa gcacacctga taacatgtac
     3961 ccatgatgag aaaaacccgc tccctctggc tagaccgcag actattggac tgcccgtacc
     4021 acgtcgcttt atgcctagcg ccgaaggagt tccgtttggt ttgccgacag ctgggcctcg
     4081 cacacaaagt gcgccctgaa tacttaccta acgagcacac agacgctgcc acacattttt
     4141 tcgttttgtc aggggggaag aaacatgctg ctgtggtgtg tttgggctgt acggaaggga
     4201 agagtcaggc acaagtagcc gctttactgg tgcatgaagc tgtacacata tggcaggaca
     4261 cactagcatt gttgggggaa gaaaaaccag gaagcgagct tgaggcgtat gccgtacagt
     4321 ccatagtaca gacgcttctg gaggcgtata ttaggagggc taagcgtaaa catggaagca
     4381 cctgaaaaga atcccgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnataa
     4501 taatcttgaa gtctctaacg ttatctgtta gagttatcaa atccatgaca gacggattgt
     4561 agtctgtcaa tcgggtcgac tccgtaaaaa gtcgtatttc gcagcttcgt cagcgacatg
     4621 ggcgagcgct tagcacagcg gattggtgcc agtgggtctt ttggactctc atgcgaatac
     4681 gattggagga cctatgtctt atacaaattt tgcacagctc acttccgagc aaaaaactac
     4741 ttggtctcgc gacctttggg cgcaagctcg aaacatgtcc tttgttaacc agtttctggg
     4801 caaggactcg aattcgatgg tgcagcacat caccgagcta aagaagagcg agaaaggcgc
     4861 acgggctgtc attactctcc tgaccgacct cgaaggtgac ggtatcgctg gcgaccgcac
     4921 gctggaaggt aacgaggagg cgatgaagag cttcgatcaa gtcattcggc tcgatcaact
     4981 ccgtcatgcc aaccgtcacg aaggtcgcat ggccgaccag aagtcagtcg tttctttccg
     5041 cgaagaatct cgtgacaagc tggcttactg gctcagcgac cgcattgacc agctcgcgtt
     5101 ccttaccctg gctggtatcg gctataccca gcgtaacgca gggggcaccc gtgtcggttc
     5161 cgaccttcag tatctcgagt ttgctgctga cgtcacgaca ccttcttcca agcgctacgc
     5221 tcggtgggac ggtgcaagta acaaggttct ttcgtggggc accggttctt cggcggtcac
     5281 tgcggcggac accccgactt ggggtatgtt tgtccaggcc aaggcatacg ccaaggataa
     5341 ctatatccgg ggcattaaga gcaagggcgg cgaagaggtt tatcacgcct tcctgtctcc
     5401 gaccgccatg gcgaagctga agctcgacga tacgtatctg cagaacttgc gttacgccgt
     5461 gactcgtggt gacgacaaca acctcttcac gggcaccagc gtgaagatcg acgggatcgt
     5521 cctgcatgag ttccgccatg ttccgaatac tcgtctcgca gcttcgggaa gcaaattcgg
     5581 cgcgagtggt acgattgacg ggtgtcagat tctgttctgc ggtgcgcagg cgctcggaat
     5641 ggccgacatc ggtaacccgg agtgggtcga aaagggcttt gactacgaga atca
//
LOCUS       DAOQLC010000201         5567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6493_length_5567_cov_16.722242, whole genome shotgun sequence.
ACCESSION   DAOQLC010000201 DAOQLC010000000
VERSION     DAOQLC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_5567_cov_16.722242"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1761)
                     /locus_tag="PL000_20115"
     CDS             complement(343..1761)
                     /locus_tag="PL000_20115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC91242.1"
                     /translation="MINLFAHQKESVKRLAASPIIFDTSDAGTGKTLPEVVDFAARRR
                     KKGKCLLVLATKSLLTSAWQAEFRKFAPDMKVSVAYAENRGKAFAEPVDVYITNHDAA
                     VWLAQQPAAFFTKFDSIVVDESTAFKHHTSQRSKALAKIIKYFKYRRLLTGTPNTNGV
                     CDLWHQVYLLDDGKRLGKSFFAFRKAACESVRTTAAPVYVVDKDKNGIPRRGVDGKPL
                     MKLITPVTWVDRPSIEMTIAALIRDITIRNKFEDCVDIPANHMYARSFSLSKAHMKAY
                     ETMRDEKILQLKRSTITAVNAAVVAGKLLQITSGAVYNDDEDYSLIASERYEMTLDLV
                     EERSHSIVFFNWSHQRNELIRLATKRGLTFAVYDGTTSSRDRTALVTDYQAGKYQVLF
                     AHPQSAGHGLTLTRGSATIWASPTYNLEHFLQGCKRIHRIGQTQRTETIMIIAEGTLE
                     EMVWQALQDKNVKLVDLLENLK"
     gene            complement(1927..2625)
                     /locus_tag="PL000_20120"
     CDS             complement(1927..2625)
                     /locus_tag="PL000_20120"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC91243.1"
                     /translation="MIRSTSMSRLQEYEACPYRSKLKLVDKIPEPERPLPPGKTEHAN
                     DRGTRIHTGCEEFINGKSTTFPIEARKFKEELHSLKSFFKKGLVSMEGEWGFSKEWQP
                     VDWRLAWLRVKLDVLVFLKPTNAVIVDWKTGSSFGNQIKHGEQTQLYTATTCIRYPAV
                     QHVTTELWYLDQDELVQNSFSREQALRFVKPFDRRFKAMTEATEFKPRPNPISCKYCP
                     YHPVRGTGDCKFGV"
     assembly_gap    2965..3064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3151..5508)
                     /locus_tag="PL000_20125"
     CDS             complement(3151..5508)
                     /locus_tag="PL000_20125"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HNC91244.1"
                     /translation="MPFKLTFLSADAPLTKTITRKADGSIKKSGYPMVGKFTSETIEI
                     RDLLHFNEALVKRANSPEKPCLLKGTINTSLVNASRAGSTKTNDTTQWVCLDLDNAKF
                     SAPEEVMRALGLGDLSYIVQYSSSYKLSTSKNLSCHIFFVLSKPVPAPRLKAWLMHLN
                     LNIPAFETGITLSSSQQCVHWPLDITACQNDKLLYVSPPIFVGMQSPVSDRERIQLII
                     KGTNALDVSKMPEHPIAGLKQKQRAKLNQLRTAAGLKPQTIKIKQVGEYEVQTGADEI
                     SKYEEIDCGEYVRFNLNGGDSQAYWHRKGDLTYLHNFKGEPSLILKEVLPAYYAEQVK
                     DQRAGNRTPSGTGDLVLCFQERVTGEYWWGTWNPEKQTLDINSVNTADKLSNAVRSYG
                     VPVPDPLPIWEKVFNPHMNETVDEEKKVVNSFKATRYMLEAKKFKKGEFPIIQRLLDS
                     AVGTGEIQDHFVNWLAVMWQFRVKPQTAWVLHGNQGTGKGVFWNRVIRKLFGYSNTSY
                     SLAHSLKEQFNGYLENALVACIDEIDADMFTDGAVVEAKLKVFITEPTLKVRRMRTDE
                     YEIPSYTGLLFFSNMPRPVSIPASDRRYNIGKFQPDRFITTSAELDKIEDELEAFTAF
                     LTAYKADPHRAAQVLDTEDRREIQRLSITSTQEIATAILKGDLEYLWSYVTNGSVSNI
                     IDPIESQYNAVVYRLTRDVLNGQKFQISREEIHTIFQLSNKDTPKGNRFTSFLRHQGV
                     TLSRLRVNGALIYGYETMVKLEPELRRMLVENLAAATKPLLRGVT"
BASE COUNT         1329 a         1331 c         1259 g         1548 t
ORIGIN      
        1 gatcaagcga cgaagagatt tgtcagttaa catgtggtgg tccttttgtc gaattgaaat
       61 tcagatgagc cgctggattt gaccgatcca aaaggactct cctttcttta tagccaggtc
      121 aatctccctg gtttgagttg cgacccacct gaattcgttt ataacgaaga aaatcttacg
      181 gaaaaggagc tactctcgac cactccgtat ccgtcaaggc aaccccaaac ttttcaacca
      241 tagcgtcgta agcttggccg tgattgagaa acggaccaag aagcagctca catggttgat
      301 acgttgcccg ttcaaacttt tctacgtaat actgacgaaa tttcatttca aattctccaa
      361 gagatcgaca agcttcacat ttttatcttg caacgcttgc cagaccattt cttcgagcgt
      421 gccctctgcg ataatcatga tcgtttcggt tctttgggtt tgaccaatac gatgaattcg
      481 cttacatcct tgaaggaagt gttcaaggtt gtatgttgga gaggcccaga ttgttgcact
      541 tcctcttgta agggtgagcc catgtcctgc cgattgtgga tgcgcaaaca gtacttgata
      601 tttccccgct tggtaatccg taacgagtgc agttcgatcc cggctgctcg tagttccgtc
      661 ataaacggca aaagtaagtc ctcgtttggt agcaagtctg atgagttcat tgcgttgatg
      721 gctccagttg aaaaatacta ttgaatgtga gcgctcttcc acaagatcga gagtcatttc
      781 atatctttca gaggctatga gagagtagtc ctcgtcatcg ttgtaaactg cgcctgacgt
      841 gatttgcaac aacttccctg ccactacggc agcattcaca gctgtgattg tggacctttt
      901 taactgcaaa atcttttcgt cacgcatcgt ttcatacgcc ttcatatgcg ctttcgaaag
      961 cgagaagctc cttgcataca tatgattggc agggatatcg acgcaatcct cgaacttgtt
     1021 gcggattgta atgtcacgaa tcaatgcagc gatagtcatc tcgatactgg gtcggtcgac
     1081 ccaagtaacg ggtgtaatca acttcattaa cggttttccg tccactcctc tgcggggaat
     1141 tccgtttttg tctttgtcaa caacatatac tggtgcagct gtggtgcgaa ccgattcaca
     1201 cgctgctttg cgaaatgcga aaaagctctt acccagccgc ttcccatcat cgagcagata
     1261 aacttgatgc cagagatcac acacgccatt tgtatttggt gtaccggtta gcagtctgcg
     1321 atatttgaaa tacttgatga tcttcgccaa agctttgcta cgttgactgg tatgatgctt
     1381 aaacgctgtg ctttcgtcaa cgacaatgct gtcaaactta gtaaagaacg ctgctggttg
     1441 ttgagccagc cagacggctg catcatgatt ggtgatgtag acatccaccg gttcagcaaa
     1501 cgctttgcca cgattctctg catacgccac tgataccttc atgtctggtg cgaacttgcg
     1561 gaattctgct tgccacgcgc tagtcaacaa tgattttgtt gccagaacaa ggagacattt
     1621 acccttcttg cggcggcgtg ctgcgaaatc aactacttcg gggagggttt tacctgtacc
     1681 tgcatcgctg gtgtcgaaga taatcgggct tgctgcgaga cgtttaacgc tttccttctg
     1741 atgtgcgaat aagttgatca ttgaattctt gcagctcctt cgaccatatg aacagtgtgt
     1801 tgttttgaga accacgatat ataggaatcg gcccggacgg aggcggccgt atactataaa
     1861 catcccaact cacaggggtg gcgacatcgt accctctttc atccgttcgt gctttggcag
     1921 ttagtttcat actccaaatt tgcagtcgcc tgtcccacgc acaggatggt atggacagta
     1981 tttgcaactg atggggttag gtcgcggttt aaattcagtc gcttcggtca tcgctttgaa
     2041 acgcctatcg aagggtttga caaagcgaag agcctgctct ctcgaaaaag aattctgtac
     2101 caactcatcc tgatcgaggt accaaagctc cgtagtcacg tgttgtaccg ctggataacg
     2161 aatgcaggtt gttgccgtat aaagctgtgt ctgctcgccg tgtttgatct ggttaccgaa
     2221 gctcgagcct gttttccagt caactatcac ggcatttgtg ggtttcaaga aaaccaggac
     2281 atcgagcttc acgcgcagcc atgcgagacg ccagtcgact ggctgccact ctttggagaa
     2341 cccccattct ccctccatag acactagccc ttttttgaaa aaggacttga gactatgtaa
     2401 ttcttctttg aacttcctcg cttcgatggg gaaggttgtc gatttaccgt tgatgaactc
     2461 ttcacatccg gtatggatgc gtgtgcctct gtcattggca tgctccgtct tgcctggagg
     2521 aagaggtctt tctggttcag gaatcttgtc aaccagtttc aacttggagc gatacgggca
     2581 ggcctcgtat tcctgcaaac gcgacatact tgtgctgcgg atcattggag gctcctatat
     2641 gaaaacgtca gagtgtcaca cagctactta aacattgcaa ggggaagttg ctctgtgtac
     2701 agctcagaga taccgagatc attaacccac gctcgggcct cggcaagagt ctcaaagtgc
     2761 atcggttctt ggcttatttg gttgatacag ctcctccaca agcatgccca aacaagaatt
     2821 tgcacgatga agcgagacgc tttgggatca aaaatgattc gatacatgat ttagtccttt
     2881 gtaattccga caaggagaac tccacaagcg acaatgcaag caaagactaa caagatttcc
     2941 agaatgacca tactacctcc agagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnngtataa tgcatcacat acaccgatgg taacatagtc ttcaatcagc ctacgggctt
     3121 ccatgtagag ccctcgcttg tagtcgtcca tcatgtaact cctctgagta atggtttcgt
     3181 tgctgcagca agattttcaa ccaacattct tcgcaactct ggttccagtt tcaccattgt
     3241 ttcgtacccg taaatcagag ccccattcac tcgcaagcgg cttagcgtaa caccctgatg
     3301 acggagaaag cttgtaaatc tgtttccttt cggagtgtcc ttattgctta attgaaaaat
     3361 tgtatgtatc tcctcacgag aaatttgaaa cttctgccca tttaacacgt ctcgagtaag
     3421 gcggtagact accgcgttgt attgcgattc aattgggtca atgatattcg atacactccc
     3481 gttggttacg tacgaccaga gatattccaa gtcaccctta agaattgccg tggcgatttc
     3541 ttgtgtactg gtgatggata agcgttgaat ctcccggcga tcttcagtgt ccagtacctg
     3601 cgctgcacga tgagggtctg ctttgtatgc tgtaaggaat gcagtgaaag cttccaattc
     3661 atcttcgatc ttatccaatt ccgcagaagt tgttataaat cggtcaggtt gaaactttcc
     3721 aatgttatat cttcggtctg acgccggaat tgatactggt cgaggcatgt tggagaaaaa
     3781 cagaagccct gtgtaacttg ggatttcata ctcatcagtg cgcattcttc gcactttgag
     3841 cgttggttcc gtaatgaaaa ctttcagctt agcttcaacc accgcgccgt ctgtgaacat
     3901 gtccgcatcg atctcatcga tgcaggctac cagggcattc tccaagtatc cattgaattg
     3961 ctccttcaaa gaatgcgcca agctgtagct tgtattggaa taaccgaata atttacgaat
     4021 cacacggttc caaaatacac cctttccagt tccttgatta ccgtgaagta cccatgcagt
     4081 ttggggcttt acgcgaaact gccacatcac agcaagccag ttaacgaaat ggtcctgaat
     4141 ctcccctgta cctacggcac tatctagcag cctctgaata atgggaaatt cacccttctt
     4201 aaacttcttg gcctccaaca tgtaccgcgt cgccttgaaa ctgttaacaa cctttttttc
     4261 ctcatcaacc gtttcgttca tatgaggatt aaataccttt tcccaaattg gaagtgggtc
     4321 tggtacagga acaccatagc tccgaacagc gttgctcaat ttatcagcgg tgtttaccga
     4381 gttgatatcc aatgtctgtt tttctgggtt ccatgtaccc caccagtact caccggtcac
     4441 tctttcttga aagcacagga ccaaatcccc tgtaccagaa ggtgtgcggt ttccagcgcg
     4501 ttgatctttg acttgctcgg cgtaatatgc aggtagaacc tctttcaaaa tcagactggg
     4561 ctcacctttg aaattatgga gataggtcaa atccccttta cgatgccaat atgcttgact
     4621 gtctccacca ttcaagttaa agcgaacgta ctccccacaa tcgatttctt catatttcga
     4681 aatctcgtcc gcacctgttt ggacctcata ctcaccaacc tgcttgatct tgatagtttg
     4741 cggctttaat ccagcagctg tgcggagttg attaagtttt gcacgttgtt tctgcttcag
     4801 tccggcaata gggtgctcag gcatcttgct aacgtccagt gcgtttgtgc ctttaataat
     4861 gagctgaatg cgttctctgt ctgacactgg agactgcata ccgacaaaga tcggcgggga
     4921 cacatagagc aatttgtcgt tttgacaggc tgtgatatcc agtggccaat gcacacactg
     4981 ctgactggaa gacagagtaa tcccagtctc gaaagctggg atattgagat tcagatgcat
     5041 gagccacgct ttgaggcgcg gtgcaggaac aggcttgctc aaaacgaaga agatgtggca
     5101 tgacaggttc tttgatgtcg agagcttgta gctcgagctg tactgcacga tgtagctcag
     5161 atcgcccaag cccagggctc gcatgacctc ctcgggagca ctgaacttgg cgttgtccag
     5221 gtccagacat acccattgag tcgtgtcgtt cgtcttcgtt gagcctgccc ggcttgcgtt
     5281 gacgagagag gtgttgattg ttcctttgag cagacagggc ttctcaggac tgttcgctcg
     5341 tttgaccaga gcctcgttga aatgtagtaa gtccctgatt tcaatggtct cactggtgaa
     5401 cttgccgacc atagggtagc cagacttttt gatcgaccca tccgccttgc gggtgatcgt
     5461 cttggtcaag ggggcatcgg cgctaaggaa tgttaatttg aatggcatat atcccccgtt
     5521 ggtagtagac ctcctagtct gtcagagggc gtgagagcgt gtcaagc
//
LOCUS       DAOQLC010000202         5508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6622_length_5508_cov_26.584266, whole genome shotgun sequence.
ACCESSION   DAOQLC010000202 DAOQLC010000000
VERSION     DAOQLC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6622_length_5508_cov_26.584266"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..885)
                     /locus_tag="PL000_20130"
     CDS             complement(4..885)
                     /locus_tag="PL000_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNC91245.1"
                     /translation="MAREIKFGNRMMGDGHPAYVIAEVGINHNGDLDIAKKIIDAAAH
                     AGADAVKFQKRTPEVCTPPEQQKQMRETPWGYITYLDYRYKVEFSEEQYREIDRYCKE
                     KKIDWMVSVWDEPSVDFMEKFDTPAYKVPSASLTDHNLLKYVRKTGKPVIISTGMSTM
                     DQIKRGVEVVGEDNLVIMHCTSTYPCEPEELNLNMIGTLRREFPNNPIGYSGHEVGLV
                     PSAVAIALGATSIERHITLDRAMWGSDQAASVEPGGFERLVKYIRVTEAAMGDGVKKV
                     YESEKGSMKKLRRVVSE"
     gene            complement(875..2119)
                     /locus_tag="PL000_20135"
     CDS             complement(875..2119)
                     /locus_tag="PL000_20135"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HNC91246.1"
                     /translation="MTDILALIPARGGSKGIPRKNIRSFAGYPLIAWSIAAAKQSELV
                     TRVIVSTDDEEIAAVAREWGAETPFMRPAEFAQDKTTDLPVFEHALKWLEDVEGYRPD
                     IVIQLRPTSPVRPHKMVDDAIRILLEHPDADCVRGVVPAGQNPFKMWRFHGEGKPLNP
                     LLTVDGIAEPYNAPRQILPPVYWQTGHIDAIRTATIAQKVSLTGDVIYPLVIDPKYTV
                     DIDTLPDWAKYEALVYSGLEMVSPGAPRRLMPETVKMIICDFDGVVTDNLVITDQDGR
                     ESVTASRSDSMHIKKLREMGVEVMILSSEPNPVVLARAKKMGVEAIHNVGMQDKGRVM
                     REVLAQKNVKAQNVVYVGNDLNDLPCFEIAGWSVAVADAYPEVIRAADHVLTKTGGHG
                     AIRELCEIVLKQLSQKENTNGS"
     gene            complement(2165..2992)
                     /locus_tag="PL000_20140"
     CDS             complement(2165..2992)
                     /locus_tag="PL000_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC91247.1"
                     /translation="MTIFDKFSLKDRVAVVTGGVGLLGAEFCKTLAEAGAAVAVVDLN
                     GEAASKVADGLVRSGYKAMGIATDITKPESVNAMVASVLSTFGRLDVLVNSAALDPKF
                     DPDAAAKGIAPGAFEDYPLEQWNAAMNVNLTGMFLVTQACVKQMNAQGKKGSIINICS
                     TYGLNGPDQRIYIKDGKRVAYKPVYYTTTKAGVMGFTKYLAAYYAETEIRVNALTPGG
                     VFNNHEEYFVKNYSAKTILGRMAKKDEMNGALLFLASDASSYMTGNNVIVDGGWTAW"
     gene            complement(3029..4666)
                     /locus_tag="PL000_20145"
     CDS             complement(3029..4666)
                     /locus_tag="PL000_20145"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNC91248.1"
                     /translation="MPLNCGAADQRHGSDSISVETCQVFLSFMTRQRAASFATLGLLA
                     ILGTVFILYATPEGLGLSDDSIAYIAGARSLLSGQGYREAWLASNGPVTHFPPAFSSI
                     LALIGLSGLDPVRGARFLNASLFGANTFLLGLIGWRMTKSQAAGFVLALLFVLNASLL
                     RIHAVAMSEPLYIFFSLGAFLLFEFYIEQREKRWLILAGCFVGAAYLTRYAGLALFAT
                     FLAALILLRENWLKRITRSLIFIASFFPWAAAWSFRNKLVADNATNRTLVYHPLTEEN
                     IQAGIYNFSEFLVPIEAWRRALVKNLDLLFVILAILGLIILVWVAVTGLKKFFKPSSP
                     MPEILSFTNGLYVFAYLASILSSMMLFDASTKFKARINAPIYISLLILLVWFGFWLWQ
                     KRTLLWRGIVVGAAVFLVGLSSRELVGVVRQLHKGGQGYASFQWYDSAAMDFLSALPE
                     GIRIYTNQAGPVYLYTNRPAYVLPDLVDPVTGLPRERYEEGVQALQEDVLSGNAVLAL
                     FKFGAEEEDVQGIYLQLAEGLNLALDKRGDKIYTNQP"
     gene            complement(4693..>5508)
                     /gene="cysC"
                     /locus_tag="PL000_20150"
     CDS             complement(4693..>5508)
                     /gene="cysC"
                     /locus_tag="PL000_20150"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HNC91249.1"
                     /translation="FYGPYDAQEIMKQYEAELGVKMVPFEMLVYLPDEDRYVEEKDVP
                     KGAKVANISGTQVRDDYLAKGKLLPEWFTRPETAEILREMYPPRHKQGFAIWFTGLSG
                     SGKSATTAVLTTLLLERGRETAILDGDVVRTHLSKGLGFSKEDRDTNILRIGFVAGEI
                     AHAGGIVICAAISPYRATRQEARKMVGENFIEVFMDTPVEVCEQRDVKGLYAKARQAM
                     QDGKPMGFTGVDDPYEPPIDPELTLKGFGASPEENAHLIVKYLEEQGYILPVK"
BASE COUNT         1315 a         1526 c         1436 g         1231 t
ORIGIN      
        1 ttcttattcg ctcaccacgc ggcggagctt cttcatggag cctttttcag attcgtaaac
       61 cttcttcacg ccatcgccca ttgccgcttc ggtaacgcgg atgtatttca ccaggcgttc
      121 gaaaccacca ggctcaacag acgcagcttg atcactgccc cacatggcgc ggtcaagtgt
      181 gatgtgacgt tcgatggatg tggcgccgag cgcaatcgca actgcggaag gcacaagccc
      241 cacttcatgg cctgaataac cgatggggtt gtttggaaat tccctgcgca gggttccaat
      301 catgttgagg ttcaattcct caggctcgca tggatacgtg ctggtgcaat gcatgatgac
      361 aaggttgtct tcaccaacca cttccacgcc gcgtttgatc tggtccatgg tggacatgcc
      421 cgtggagatg atgacaggct tgcctgtctt gcgaacatat ttcaagaggt tgtgatcggt
      481 cagagaagcg gaagggacct tgtaggcggg cgtatcgaac ttctccatga aatccacaga
      541 gggttcatcc caaaccgaaa ccatccaatc gatcttcttt tctttgcaat agcggtcgat
      601 ctcgcggtat tgttcttcgc tgaactcaac cttgtagcga taatcgagat acgtgatgta
      661 accccagggc gtctcgcgca tttgtttctg ttgttcgggc ggggtgcata cttcgggtgt
      721 gcgtttttga aacttcaccg cgtctgcgcc tgcatgagcg gcggcatcaa tgatcttctt
      781 tgcaatatcc aaatcgccat tgtggttaat gccaacttcc gcgatcacgt acgcagggtg
      841 accatcaccc atcattcgat ttccaaattt aatttcacga gccattcgta ttctcctttt
      901 gagataattg tttcaataca atttcgcaca actcacggat cgctccgtgg ccgcctgttt
      961 tcgtcaacac atgatcggcg gcacggatga cttcgggata tgcatccgcc accgctaccg
     1021 accatccagc gatctcaaaa cagggaaggt cgttgaggtc attgccgaca tagacgacgt
     1081 tctgcgcttt gacattcttc tgcgcgagga cttcgcgcat cactctacct ttgtcctgca
     1141 tgcccacatt gtggatcgcc tccaccccca tctttttcgc acgcgccaac accaccgggt
     1201 tcggctccga agagaggatc atcacctcga cgcccatctc gcgcagtttt ttgatatgca
     1261 tgctgtcact gcgtgaggct gtgaccgatt ccctgccatc ctgatcggtg atgacgagat
     1321 tatccgtcac tacgccgtcg aaatcgcaga tgatcatttt cacggtttca ggcatcaggc
     1381 ggcggggggc acccggagag accatttcca aaccgctgta cacaagcgct tcatacttgg
     1441 cccaatcggg caatgtatca atatccactg tgtatttcgg gtcgatcacc agcggataga
     1501 tgacatcacc agtcagggat accttttgcg cgatcgttgc cgtacggatc gcatcaatgt
     1561 gcccggtctg ccagtaaacg ggcggcagaa tctggcgcgg agcattatat ggttcggcaa
     1621 ttccatccac ggtcaaaagc ggattcagag gtttgccttc accgtgaaaa cgccacatct
     1681 tgaaagggtt ctgcccggca ggcaccacac cgcgcacaca atcggcatcg ggatgctcca
     1741 gcaaaatgcg aatggcatcg tccaccatct tgtgcggacg gacgggagag gtggggcgaa
     1801 gttggatcac aatatcggga cgatagcctt ccacatcttc gagccacttg agagcatgct
     1861 caaatacggg cagatcggtg gtcttgtcct gtgcaaattc ggcggggcgc atgaagggag
     1921 tctctgcccc ccactcacgg gcaaccgcag cgatctcctc atcatctgtc gagacgatga
     1981 cacgcgtcac caactcagat tgttttgccg cggcaatgct ccacgcaata agcggatacc
     2041 ccgcaaaact gcggatattc ttgcgcggaa ttcctttcga accaccgcgg gcggggataa
     2101 gagcgagaat gtctgtcata gttttcttgc agcgacacag cggcgccgtg tcactacctt
     2161 tgttttacca cgccgtccag ccgccatcta caatgacatt attgcccgtc atgtaggaag
     2221 aagcatcaga tgccaggaac aataatgcac cgttcatttc atccttcttt gccatgcggc
     2281 caaggatggt cttggcggaa taattcttca caaaatattc ttcatggttg ttaaacacac
     2341 cgcccggagt gagcgcattc acgcggatct cggtttcggc ataataggcg gcaaggtatt
     2401 tggtaaatcc catcacacct gctttggtgg tggtgtaata cacgggctta tacgccacac
     2461 gcttgccgtc tttgatgtaa atccgctgat cggggccgtt gagcccatac gtagagcaga
     2521 tgttgatgat gctgcccttt ttgccctgcg cattcatttg cttgacacaa gcctgcgtta
     2581 cgaggaacat ccccgtgagg ttgacattca ttgccgcgtt ccattgctca agcggataat
     2641 cctcgaaggc accaggggca attcccttgg cggcggcatc ggggtcgaat tttggatcaa
     2701 gggcggctga attcaccagt acatcgagcc tgccaaaggt cgaaagaacc gaggcgacca
     2761 tcgcgttgac cgattctggt ttggtgatat cggtcgcgat tcccatcgcc ttgtatccag
     2821 atcggacgag tccatctgcc actttgctgg ctgcctcacc gttcaggtca acaacagcaa
     2881 ctgccgcgcc tgcctcggca agggtcttgc aaaattccgc gccgagcagc cccaccccgc
     2941 ctgtcacaac cgctacgcgg tctttcaaac tgaatttatc aaaaatggtc atgtgatttc
     3001 cttatgtagc gcgacatagt atgtcgcgtt acggctgatt tgtataaatc ttatcgccgc
     3061 gtttatcgag agccaggttc aaaccctcgg ccaattgcag atagatgcct tgtacatcct
     3121 cttcttccgc gccgaacttg aacaaagcga ggacggcatt ccctgaaagt acatcctcct
     3181 gcaaggcctg tacaccttct tcgtaacgtt cgcgcggcag ccctgtcaca gggtcaacca
     3241 gatcgggcaa aacataggcg ggacgattcg tgtaaaggta aacggggcca gcctgattgg
     3301 tgtaaatacg aatgccttca ggaagcgcac tcagaaaatc catcgcagcc gaatcatacc
     3361 attggaagga tgcatatccc tgcccgcctt tgtgaagctg acgcacgact cccaccaact
     3421 cgcggctgga tagccccaca agaaacacag ccgccccgac gacgatcccg cgccaaagca
     3481 gggtccgttt ttgccagagc cagaacccga accacacaag caggatcaac agcgaaatgt
     3541 agatcggcgc attgatccgc gctttgaatt tggtgctggc atcgaacagc atcatcgaag
     3601 aaagaatgga cgccagatag gcaaaaacat aaaggccatt cgtgaaggac aaaatttcgg
     3661 gcattggtga agagggtttg aaaaatttct tcaacccggt cacagccacc caaacgagaa
     3721 tgatcagccc gagaatggca agaatgacga acagcaggtc caggtttttt accaaagccc
     3781 gccgccaggc ctcgatcggg acgagaaact cggaaaagtt gtaaatccct gcctgaatat
     3841 tctcctcagt cagcgggtga tagacgagcg tacggtttgt ggcattatct gccaccaatt
     3901 tattacgaaa actccacgcc gccgcccacg ggaaaaacga ggcgatgaag atcagcgaac
     3961 gggtgattcg cttcaaccag ttttcacgca gcaaaatcag cgccgcgaga aaagtggcga
     4021 acaaagccag ccctgcgtaa cgcgtcaggt aggccgcccc gacaaaacag cccgccagaa
     4081 tcagccagcg tttttcacgc tgctcgatat aaaattcgaa caaaaggaat gcgccaaggc
     4141 tgaaaaagat atacagcggc tcgctcatcg ccacggcatg gatgcgcaac agcgaagcgt
     4201 tcaacacaaa aagcagggcc agcacaaacc ccgccgcctg cgacttggtc atgcgccagc
     4261 cgatcaggcc gagcaaaaaa gtatttgcgc cgaacaaaga cgcgttaaga aagcgcgcgc
     4321 cgcgtacggg gtcaagtccg ctgaggccga tcaatgcaag aatggatgaa aaggctgggg
     4381 gaaagtgtgt gaccgggccg ttactcgcaa gccatgcttc gcgatagccc tgtccgctta
     4441 atagactgcg tgcgcctgcg atgtaagcga tcgaatcatc tgaaaggccg aggccttcgg
     4501 gagtcgcata aaggatgaaa actgttccca gtatggcgag caggccgagg gtggcgaaag
     4561 atgcggcacg ttgtcgggtc ataaaagata aaaagacctg acaggtttcc acagagatgg
     4621 aatcgcttcc atgtctctga tccgccgcac cgcaattcag cggcatggcg caaaacctgt
     4681 caggtcttgg gtttacttca caggcagaat gtagccctgt tcttcgaggt acttgacgat
     4741 gaggtgggca ttctcttcag gagaagcgcc gaaacccttg agggtgagtt cgggatcgat
     4801 cggcggttca tagggatcat ccacaccggt aaagcccatc ggcttgccat cctgcatggc
     4861 ctggcgggcc ttggcataca aacccttcac atcgcgctgt tcgcagactt caacaggggt
     4921 atccatgaag acttcgatga agttctcacc gaccatcttg cgggcttcct gacgggtggc
     4981 gcggtagggg ctgatcgccg cgcagatgac gatgccgcct gcatgggcaa tttcgccggc
     5041 cacaaagcca atgcgaagaa tgttggtatc gcggtcttcc ttgctgaaac caagaccctt
     5101 ggaaaggtgc gtacgaacca catccccatc gaggatggcg gtctcgcggc cacgttcgag
     5161 gagcagggtg gtaaggacgg cagtggtggc cgatttgcca gagccgctca aaccagtgaa
     5221 ccagatcgca aatccctgct tgtggcgggg cggatacatt tcacgcaaaa tttcagcggt
     5281 ttcggggcgg gtgaaccatt cggggagcaa tttgcccttg gcaagatagt catcgcgcac
     5341 ctgagtaccg gagatattgg caactttcgc gcccttcggc acatcttttt cttccacata
     5401 acgatcttca tcgggaagat agaccagcat ttcgaacgga accatcttga cgcccagttc
     5461 ggcttcgtac tgcttcatga tctcctgcgc atcgtacgga ccgtagaa
//
LOCUS       DAOQLC010000203         5503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6634_length_5503_cov_15.705764, whole genome shotgun sequence.
ACCESSION   DAOQLC010000203 DAOQLC010000000
VERSION     DAOQLC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6634_length_5503_cov_15.705764"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..886)
                     /locus_tag="PL000_20155"
     CDS             complement(320..886)
                     /locus_tag="PL000_20155"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC91250.1"
                     /translation="MTIGLFVDGSFVYKSYPDNIDYLKLRQVIEKELSDTIDEAYFFN
                     SDDDPPKAAKFNKNLTLPPPNGPGFRLKIYWLTKKKLNWPQALGGHPVLHPTDQTIQF
                     ELIQQKGVDVGLAYHMVRSFYKRHWTKLVLCAGDGEFHEPVQNLVESENVDLYLVGSL
                     RGISSELRPYARKIFEIDQEPLCSQIKR"
     gene            complement(1023..1172)
                     /locus_tag="PL000_20160"
     CDS             complement(1023..1172)
                     /locus_tag="PL000_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91251.1"
                     /translation="MEQWPDQRPNDVGWAYVAIDGEGFSEAVTVIVENERQTPAIRYI
                     EWGRP"
     gene            complement(1536..2462)
                     /locus_tag="PL000_20165"
     CDS             complement(1536..2462)
                     /locus_tag="PL000_20165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC91252.1"
                     /translation="MTAPHAIEQPAALPDGEALLPTGTQEVSVPVFTLPVEGESITST
                     FTGNTYRIGPVIGEGSFGVVYECTDTWGNPLAVKILKPLGTYEQVRDSAIAELEKLRL
                     LRHPNVTYVHDAFEFRHTFYIVFERCWQPINQFIQRDQFNGHLWLRAIARHILQAVHF
                     LHCNDYVHQDIHGGNVFVATRSDDMAPNDMIFTFMLGDLGITKMVTEMDAQNTVLAEW
                     MRAPEALAPAEYGPMDHRMDIYHCGLLFLQILLGHPLTFTTDEVLGGVPREVALRVGG
                     PFSFALEKALRRHVEFRTASALEFWGDLNTTA"
     gene            complement(2570..3643)
                     /locus_tag="PL000_20170"
     CDS             complement(2570..3643)
                     /locus_tag="PL000_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91253.1"
                     /translation="MSVLVTILKLLSTTLAGAFGILALAVEYKHEGNITKWGKIALAG
                     ILVSTAIAIVTQSLEFLSAEFDARKSALEARARLEQSERILQNLNTLTRASQPLGNPE
                     FTVVLDFPKELPQGSRFLSDVEHLASSVRSMPPTEETYSQGFIVNSRGSDGTPRSIKI
                     YPTWPGFPTKASEPELYTFLTQTSVEFSFLKNTEEKDSDIANFRKSREQPRLGYHGDI
                     GDLAFGIGGGGEPQTRTLDYDLQKKALSIWIEGSAVDRFVRRSGGIISIPDFDESIPL
                     LAVDHTMVPSLNAGDFPALRKARNEMTPRIVLVSVSGRRYVLRNWQSHENLDGYRMFS
                     GEPLQNGQEANGTKSASAASRHK"
     gene            complement(3754..5052)
                     /locus_tag="PL000_20175"
     CDS             complement(3754..5052)
                     /locus_tag="PL000_20175"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HNC91254.1"
                     /translation="MTYERDVREIEAACREVFGVSIEVAPRALQVLVTSFQRASLFGN
                     EIDLTSDLECFRRVLASSPAYADAIISKSDSLERLISTQKSTGKTSDAPRFGNFTKLL
                     SDLFVSRFPFRSGLHPVGASVKGTGLLTETDLILACLQAGRRSVLPNNTASTLLKAVG
                     LFNDGYPTERADKYLAKLEGELKSVGVVDESHAEQQIVLYLDANDHMRLQIFGANALG
                     RIEERLSKVGTFVFRGGVLQPAPSSRLFTNDAIKELEDLMNSRSTLEQEYQRFFECHP
                     EFLKSLDYKNIHAQPILYKDDGSRLIPDFFLEQVDSGWHAIADLKRSHEAMVIRKRNR
                     VYFAQWVQEAFAQLQYYREWFESPKNREAFEQAQKVSTRIYRPKMVLIAGRQSDFVDD
                     VERIRLLSAQDANLSLWTYDAVLSRAKKYREYAQAISYAL"
     gene            complement(5159..>5503)
                     /locus_tag="PL000_20180"
     CDS             complement(5159..>5503)
                     /locus_tag="PL000_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91255.1"
                     /translation="ADTRATADYANMLSCEDWPGYLSDVLRRPSRRDDIDIEIRGHEG
                     KIHPGAIRELVETYREVLKFLNSIRADIDLHPAFPELMHLIKRMEDPLRYISREMGLR
                     LPRDPLSEKSEF"
BASE COUNT         1243 a         1442 c         1466 g         1352 t
ORIGIN      
        1 gtaccctcaa tgccattcaa cctacgccgt ctccacacga aacgacatag agcccttcgc
       61 agtcacgtgc ccgctcgccc cggatgcatc acgcctcata tccggttttt attcatcgcc
      121 ccgcagtttc ggattggact tccttcagac cccgcctcac gacgacgccc ttgcccttct
      181 ccttgccttc ggctctgcga aaacctggcc ataggacttt cacccatgaa gtaacgcgcc
      241 atgcccggcg cacacgtctg aattcaccgg cgctgcgcgg cttcatcgcg cagcgtccgg
      301 tggaatgatg ggttcggcct cagcgtttga tctgactgca caaaggttct tggtcaatct
      361 cgaagatttt ccgagcatag ggtctaagct cagaagatat cccgcgcagg gagccaacca
      421 agtaaaggtc aacattctct gactccacaa ggttctgtac cggttcgtgg aactctccgt
      481 ctccagcaca tagaacaagc ttggtccaat gccgtttata gaacgagcgg accatgtgat
      541 aggccagacc tacgtctacg cctttttgct ggataagttc gaactgaatt gtttgatctg
      601 tgggatgcag gacaggatgc ccaccgagcg cttgcggcca gttgagtttc ttcttggtaa
      661 gccaataaat cttgaggcgg aagcccggcc catttggggg cggaagtgtc agatttttgt
      721 tgaacttcgc tgctttcggc gggtcgtcgt cggagttgaa aaagtaagct tcgtcgatgg
      781 tatccgacag ttccttttca atcacctgcc ttaacttcag gtagtcaata ttgtcggggt
      841 acgacttata gacgaaggag ccatctacga aaaggccaat ggtcatatga cttcctttct
      901 gctgatgagt gaagacagtg gggccgaacg tgcaagataa ccggccgccg gagcgcgcag
      961 cgcggagggg accaacaagc gcagcttgtt ggcggtccgg ttgatcgacg ggttgggcgg
     1021 cactatgggc gcccccactc gatgtaacga atggcaggtg tttgcctttc gttttctacg
     1081 atgactgtca ctgcttcaga gaaaccttcg ccatcaattg ccacatacgc ccacccgaca
     1141 tcgttgggcc gttgatcggg ccactgttcc attgttgtca ccagcattac gtgagttgca
     1201 ggagtgcctg cgtatgctgt catttcctca agttgagcct tcaactgccc ttcgtcgtta
     1261 gcttctgcac agctggaagt caaacaagcg cgagcacttt gaaaatgacc cgagacaaga
     1321 gcctcggcaa atttgactgc aagttgtgca tatggggcag agtaattgca ttccatggtc
     1381 tatcgctcga agagagaaat ggtctttgat gacgcccaac gttcgagttc aggggccggc
     1441 ggaggccgaa ggccgaaggc cggagcgaac cggcaagcgc agcttgccgg cggtcccctg
     1501 gaacgagggg ttagagcgat ttctggacaa cgagatcatg ctgttgtatt caggtcaccc
     1561 cagaattcga gggcggacgc ggttctgaat tcaacatgac gtcggagtgc cttttccaag
     1621 gcaaagctga acgggccgcc aacccttaaa gcgacttcgc gcggaacgcc ccccagcact
     1681 tcgtcagtag tgaacgttag cgggtgacca agaagaatct gcaagaagag caggccacaa
     1741 tggtagatat ccattcgatg atccattgga ccatactcgg ctggagcgag ggcttcgggt
     1801 gcgcgcatcc attcggcgag tacggtattt tgggcatcca tttcggtgac catcttggtg
     1861 atgcccaggt cgcccagcat gaacgtgaaa atcatatcgt tcggagccat gtcgtcgctg
     1921 cgcgttgcga caaagacgtt gccgccgtga atatcttgat ggacgtagtc gttgcagtgt
     1981 aggaaatgaa cggcctggag aatgtggcgg gcgatcgctc ggagccaaag gtgtccattg
     2041 aactgatcgc gctgaatgaa ctggttgatc ggctgccagc atcgttcgaa cacgatgtag
     2101 aaggtgtgac ggaattcaaa tgcatcgtga acataggtga cgttgggatg gcgaagaagt
     2161 cggagcttct ctagttcagc aatcgcacta tcgcgcactt gctcgtaggt acccaacggc
     2221 ttcaatatct tcacagccag cgggtttccc catgtatccg tacattcgta cacgaccccg
     2281 aagctgcctt cgccaatgac gggaccgatt cggtacgtat tacccgtgaa cgtgctggtg
     2341 atggattcgc cttcgaccgg tagagtaaag actggaacgg agacctcctg agttcctgtg
     2401 ggcagcaatg cctcaccgtc aggcagcgca gcgggctgtt ctatcgcgtg aggtgcggtc
     2461 ataagcggac tcaggtagga agcgctctaa cgttcgagct cacccgcctg cggaatcggg
     2521 cgcagcccgc tgtagcaagt cgggtgcagc gaggggttag gctgcgccac tatttgtgcc
     2581 tcgacgcagc tgatgcggac ttagttccat tcgcctcttg gccattctgt agtggctctc
     2641 cggagaacat gcgatagcca tccagatttt cgtgcgactg ccagttacgc aaaacatagc
     2701 ggcggccact gacggatacc aatacgatgc gtggagtcat ctcgtttctg gccttgcgaa
     2761 gtgcgggaaa gtctccagca ttcaagcttg gcaccatagt gtgatcgaca gctagcaaag
     2821 ggatgctttc gtcaaagtcg gggatagaaa tgattccgcc gctccttctc acaaacctat
     2881 ctacggcgct accttcaatc cagatggata gagccttctt ctgtaggtca tagtcgagag
     2941 ttcgagtctg aggttctcct ccgcctccaa tcccaaaggc aagatcaccg atgtccccgt
     3001 ggtagcccaa cctgggttgt tccctagact ttcgaaagtt tgcgatgtcc gagtctttct
     3061 cctctgtgtt tttgaggaat gagaactcta ccgaggtctg ggtgaggaaa gtatatagtt
     3121 cgggctccga ggccttggtc ggaaatcccg gccatgttgg atagatcttg atgctccgcg
     3181 gcgtgccatc agagcctcgt gaattgacga tgaagccttg cgaataagtt tcctcagtgg
     3241 gcggcatgga acgaacacta ctcgctaggt gctcgacgtc cgaaaggaaa cgcgatccct
     3301 gcggcaattc cttaggaaag tcaagaacta cggtgaactc aggattccct aacggttggc
     3361 ttgccctggt aagagtattg agattctgga gaattctctc ggactgctcc aggcgagccc
     3421 ttgcttcaag tgccgatttc cgagcatcaa actctgcgct taagaactca agtgattgcg
     3481 taacgatggc gattgcggtt gacacaagaa ttccggcaag ggcaatcttt ccccacttgg
     3541 ttatattccc ctcatgcttg tactcgactg caagagcgag gatgccgaag gccccggcga
     3601 gtgtcgtcga aagtaatttt aggatcgtca cgagcactga catgaacgct acctcggatc
     3661 ttggtgatgg actctcagct gccctgcgcg cctaacgtgg aggtcaccgg acgctgcgcg
     3721 gctttatcgc gcagcgtccg gtggaccgca gggttagagc gcataggaaa tggcttgtgc
     3781 atattctctg tatttctttg cacgactaag aacggcatca tatgtccata gcgaaagatt
     3841 agcgtcttga gcactcaata gccgaattct ctctacatcg tccacaaagt cagattgtct
     3901 tcctgcgatg agcaccatct tgggcctgta gattcttgtt gatactttct gggcctgctc
     3961 gaaggcttca cgattcttag gtgattcaaa ccattcccta tagtactgaa gctgtgcgaa
     4021 ggcctcttgt acccattgcg caaaatagac tcgattcctc ttccgtataa ccatggcttc
     4081 gtgcgaccgt ttcaaatctg caatggcatg ccatccgcta tccacttgct ccaagaaaaa
     4141 atctggaatt agccgtgagc cgtcatcctt gtaaagaatc ggttgagcat gaatgttctt
     4201 atagtcaagc gatttcagga attctgggtg gcattcgaag aacctttggt actcctgttc
     4261 gagcgtgctc ctcgaattca tcaaatcttc aagttctttg attgcgtcgt tggtgaagag
     4321 tctggagctt ggagctggct gaagtacccc ccctcgaaaa acaaacgtac ctacttttga
     4381 aagtcgttct tcaattcggc caagtgcatt tgcgccaaag atttggagtc gcatgtggtc
     4441 attagcgtca agataaagaa ctatttgctg ctcagcgtgt gattcatcta cgacccctac
     4501 agactttaac tctccttcca gttttgctag atatttgtcg gctcgttcag ttgggtatcc
     4561 gtcattgaac aggcctacag ctttcagtaa cgtgctcgct gtattattag gaagcacgct
     4621 ccttctgcca gcctgaaggc acgctaagat caagtcagtc tcagtcaaga gccccgttcc
     4681 ttttacgctg gcgccgacgg ggtgaagtcc agagcgaaat ggaaagcgac taacgaatag
     4741 atcggacagt aactttgtga aattgccaaa tcgaggtgcg tcgctggttt tgccagtcga
     4801 cttttgggtt gaaataagtc gttcaagtga gtccgatttg cttatgatcg catccgcata
     4861 tgcaggcgat gacgcgagta cgcgccggaa gcattcaaga tcagaagtca aatcgatttc
     4921 atttccgaat aagctcgccc tctgaaacga agtgaccaaa acttgaagtg cgcgaggtgc
     4981 cacttcaatc gacacgccga agacctctct acaggctgcc tcgatttctc ttacgtcgcg
     5041 ttcgtaggtc atctatatcg ctgctctcta acgtttgacg tgaggggccg ccgtagcggc
     5101 gaagccgcga agggaaccca caagcgcagc ttgtgggcgg tccctctcga cggaattgtt
     5161 agaactcact cttttcggac agcggatcgc gtggcagccg caaccccatt tctctgctga
     5221 tgtagcgcag tgggtcctcc atgcgcttaa ttagatgcat aagctccgga aatgctggat
     5281 gcagatcaat gtctgctcgt atggagttca gaaactttag aacttcgcgg taagtttcaa
     5341 ccagctctct gatggcgccc gggtgaattt ttccttcgtg gccacgaatt tcgatatcaa
     5401 tatcatctcg tctggacggc cgacgcaata cgtcggacaa gtagccgggc caatcctcgc
     5461 aacttagcat attcgcatag tccgcagtcg cccttgtgtc ggc
//
LOCUS       DAOQLC010000204         5481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6671_length_5481_cov_24.943789, whole genome shotgun sequence.
ACCESSION   DAOQLC010000204 DAOQLC010000000
VERSION     DAOQLC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6671_length_5481_cov_24.943789"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PL000_20185"
     CDS             <1..455
                     /locus_tag="PL000_20185"
                     /inference="COORDINATES: protein motif:HMM:NF027968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC91256.1"
                     /translation="IPLIYLGDEIGTLNDYTYLDDPAHERDSRWVHRPKTDWNQYAKR
                     NIQNTTEGRVYQGLKQLIELRKQHTVFSGGELEIIQTENDHVLGFMRTYAGKRSVIFA
                     NFSEAPQTLASRIIEQTSVTSLRPLHGRSKLTNYRELKLEPLDFVVFG"
     gene            498..2021
                     /locus_tag="PL000_20190"
     CDS             498..2021
                     /locus_tag="PL000_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNC91257.1"
                     /translation="MLSRVYSCAVVGLEGVIVEVEVDYTNGLPGVTIVGLPDAAVQES
                     RERVQTAVKNAGLHFPRHRIVVNLAPAVVRKEGPAYDLPIALGVIILAGHLPHDVMDG
                     ALVIGELSLDGVVRHTRGILPMAATARANGYKRMFVPEVDAPEAALIPGLEIIPVKSL
                     ADLYDHLAGRRLIQPYQPSESVLEPLFTPTDFAEIKGQEHVKRALEVAAAGGHNVLMV
                     GSPGAGKTLIARALPGILPEMSIEESLDVTRIYSVADQLPAGTPLIKQRPFRAPHHTI
                     SHAGLVGGGNIPKPGEISLAHRGVLFLDEFPEFGTRVLEVMRQPMEDKVVTISRAKGS
                     LTFSANFQLIAAMNPCPCGFYGDQQKACTCAPAVVTKYQKRISGPLLDRIDIHIEVPR
                     VDYEKLSGNKLGETSETIRKRVQTARDIQNQRFANTTDIVCNADMRIGEVRQFCQLQA
                     EGQSLMRAAMSQLNLSARAYHRILKLSRTIADLAGSEEILSTHLAEALQYRPKIMMG"
     gene            complement(2133..3035)
                     /locus_tag="PL000_20195"
     CDS             complement(2133..3035)
                     /locus_tag="PL000_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC91258.1"
                     /translation="MKKIFKWIGIVLGSLIGLVLLAGVTLYFVGVSRVNKVYDFPPSG
                     IVVPTDAESIARGEHIAEVTCLGCHGKDLGGVSPFFAMEGVASMDTPNLTMGEGGLGS
                     IYSDEDFVRAIRHGINQEGRANSMPPVPYFQYMSDEDLGAVIAYIRSVPPVDKTTKPF
                     QAEPLGYILFAAGMFGEQAADVISHQNNITAPEPGVTVEYGEYILRISACQDCHGVDL
                     AGGPYPDPSVTYIVPNLTPGGDLATWTEEQFLQAIRTQKTPDGRGLNANLMPFDDVNN
                     LTDEELKAIFLYLQSLPKIETQIK"
     gene            complement(3059..3754)
                     /locus_tag="PL000_20200"
     CDS             complement(3059..3754)
                     /locus_tag="PL000_20200"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC91259.1"
                     /translation="MNTQSLKVIGAGLGRTGTESLKQALEILGFGACYHMFELTMKHP
                     EHLPEWEKLVAGEKPNYEFLFDGYQSTADFPACMFYREFTAQYPQAKVVLTVRDADKW
                     YDSASKTIFRGVPAPMVAFVRFMGLFSKGARAALPTLIFAKEVVNNHFFEGRTDDRDH
                     TKAIFNAWNEDVQRTVPPDRLLVFDVKDGWEPLCHFLGVPIPSAPFPHANSGEDFKKN
                     NVRRFFNEKTTSK"
     gene            complement(3778..5343)
                     /locus_tag="PL000_20205"
     CDS             complement(3778..5343)
                     /locus_tag="PL000_20205"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC91260.1"
                     /translation="MNKITDLSATQIAQGIKSRKFSAREAVDAHIEHIETVNPKINAV
                     VVPMFDEARKAADEADAAQKRGAALGALHGVPITIKEQFLVKGTPTTMGLPSQKNHRA
                     EEDGPLVKRLRDAGAIILGKTNVSQMLMFIESDNPLYGRTNNPWNLERSCGGSSGGEA
                     AIIAAGGSPLGLGGDFGGSIREPAHFCGIQGLKPTAFRLTNFDTRSDLLPRGQEVIIP
                     QPGPMARKVADLALAMDVLAAPGQGLLDPSIPPVPWRNFADVDIKSLRVAVYEDDGYF
                     KAAPAIRRVVREAAEALRGMGAQVVEWTPPAVNELTLLFYSVFKSDDGAALRHALRND
                     EPMPQLKGIIRSKQMPGFMKKLFVKRLKSAGEERFLRIFEAIGMRSAEGYWKLVVERN
                     QHRLRFMRDLEAGGFDAIICPPTSLPAVLHGSTNDLLDFDSYARLFNVLGMPAGVVAA
                     GRVRAGEESDRQPSRDSVEQAALRVETGSVGLPVGVQVAARHWREDVALAVMSALENH
                     FESQSDYPTRPVL"
BASE COUNT         1256 a         1602 c         1372 g         1251 t
ORIGIN      
        1 gaattccgct catctacctc ggcgacgaga tcggcaccct caacgactac acctacctcg
       61 acgaccccgc ccacgaacgc gatagccgct gggtgcatcg tccgaaaacc gactggaacc
      121 aatacgcaaa acgcaacatc caaaacacga ccgaaggacg cgtgtatcag gggttgaaac
      181 aactcatcga actgcgcaaa cagcataccg ttttttcagg cggcgaactc gagatcatcc
      241 agaccgaaaa cgaccatgtt ctgggtttca tgcgcactta tgcgggcaaa cgctccgtca
      301 tttttgcgaa cttctccgaa gcaccgcaaa ccctcgcctc tcgcatcatc gaacaaacct
      361 ctgtcaccag ccttcgccct ctgcacggac gaagcaaact caccaactat agagagctta
      421 aactcgaacc actcgatttc gttgtctttg ggtaaacgta ttcatcacaa agcccaaaca
      481 tcatgtaaaa taaaaccatg ctctcccgag tttactcctg cgctgtagtg ggacttgaag
      541 gcgtcatcgt tgaagtggaa gtggattaca ccaacggtct gccgggcgtc accattgtag
      601 gcctgcccga tgccgccgtg caggagagtc gcgagcgtgt acaaaccgca gtcaagaatg
      661 cgggtttgca ttttccccgt catcgcattg ttgtcaatct tgcaccagcc gtcgtacgca
      721 aggaaggccc cgcttacgat ctgcccatcg cgctcggtgt catcatcctc gccgggcatc
      781 tcccccatga tgtcatggat ggcgcacttg tcatcggcga actttcactg gatggcgttg
      841 tgcgtcacac gcgcggcatt ttgcccatgg ccgccacagc ccgtgccaat ggctacaagc
      901 ggatgttcgt gcctgaggtg gatgcgcccg aagcagcctt gatccccggc ctcgaaatta
      961 tcccagtcaa atcacttgcg gatctttacg accatttggc gggtcggcgc ctcattcaac
     1021 cgtatcaacc ctcagagagt gttctcgaac cgctcttcac cccaacagac tttgctgaaa
     1081 tcaaaggaca ggaacacgtc aaacgcgcgc ttgaggttgc agctgctggc ggacacaatg
     1141 tgctgatggt tggcagccct ggtgctggca aaacattgat tgcccgtgcc ttgcccggca
     1201 tcctgccaga aatgtccatc gaagaatcgc tggatgtgac ccgcatttat tcagtggcag
     1261 atcaactccc tgctggcaca ccactcatta agcagcgtcc attccgcgca ccgcatcaca
     1321 ccatttcaca tgcaggtctc gttggcggcg ggaacattcc aaagcccggt gagatatccc
     1381 tcgcccatcg cggggttttg ttcctcgatg aatttcctga attcggcaca cgtgtcctcg
     1441 aagtcatgcg ccaacccatg gaagataaag tcgtcaccat cagccgcgcc aaaggctcat
     1501 taactttttc agccaacttc caattgatcg cagccatgaa cccctgtccc tgcggattct
     1561 acggagacca gcaaaaagcc tgcacctgcg caccagccgt ggtgaccaaa taccaaaaac
     1621 gcatctccgg tccattgctc gaccgcattg acattcacat cgaagttccg cgtgtggatt
     1681 acgaaaagct cagcggaaac aaactcggcg aaaccagcga aaccatccgc aagcgtgtgc
     1741 aaaccgccag agacattcaa aatcaacgct tcgccaacac cacagatatc gtctgcaatg
     1801 cagacatgcg catcggggag gtacggcaat tttgtcagtt gcaggccgaa ggtcagagtt
     1861 tgatgcgggc ggcgatgagt cagcttaatt tatcggcacg cgcttatcac cgcatcctca
     1921 aattatcccg taccattgca gacctggctg ggagcgaaga gattctgtcc acgcatctgg
     1981 cagaggcgct gcaatatcgt ccaaagatca tgatgggatg aaccacggat aattggttta
     2041 aaaataaaat cactgaagag gtggttgttt tgaacataca ggaacaagtc ttggataata
     2101 aaagacttgt tcctgtatgt ttccagacaa agctatttta tttgtgtctc tattttcggc
     2161 agtgattgca ggtacaggaa gatcgctttc aactcttcat cggtgaggtt gttgacatca
     2221 tcaaaaggca tcaggtttgc attcagcccg cgcccatcag gggttttttg tgtacgaatt
     2281 gcctgaagga attgctcctc cgtccatgtc gccaagtccc cgccgggtgt caggttcgga
     2341 acgatatagg tcacgcttgg atcagggtat ggaccacccg ccaagtccac gccatggcag
     2401 tcctgacagg cgctgatgcg aaggatgtat tctccatatt caaccgtcac accaggctcc
     2461 ggtgcagtga tgttgttttg gtgcgaaatg acatccgccg cttgttcgcc gaacatccct
     2521 gcggcaaaaa gaatgtagcc gagcggttct gcctgaaacg gcttggtcgt tttatccaca
     2581 ggcggaacgc ttcgaatata agcaatgacc gcgccaaggt cttcatcgct catgtactgg
     2641 aagtacggca cgggcggcat ggagtttgcc ctgccttcct gattgatccc atggcggatc
     2701 gcacggacaa aatcttcatc ggaatagatc gaacccagcc caccctcacc catcgtaagg
     2761 ttgggcgtgt ccatggatgc aacaccttcc atggcaaaga aaggactgac gccccccagg
     2821 tcctttccat gacagccaag gcaggtcact tccgcaatat gttcgccgcg cgcgatgctt
     2881 tccgcatctg tgggaacgac gatacccgaa ggcggaaaat catagacctt gttgacacgg
     2941 gatacgccga caaaatagag cgtgactccc gccagcaaga ctaatccgat caacgagcca
     3001 agcacaatac cgatccattt gaatattttt ttcattgcta attctccttt ggcgattctc
     3061 atttggaagt tgttttttca ttgaaaaacc gtctgacgtt gtttttcttg aaatcctcac
     3121 cactattggc atggggaaaa ggcgcggatg gaatcggcac gcccaggaaa tggcagagcg
     3181 gctcccaccc atctttcaca tcaaagacca gcaggcgatc tggcggcacg gtgcgctgaa
     3241 catcctcatt ccaggcgttg aagatcgctt tggtgtgatc tcggtcatcg gtacgacctt
     3301 caaaaaaatg attattaacc acttctttgg caaagatgag agttggcaaa gcagcgcgcg
     3361 caccctttga aaacaagccc atgaaccgaa cgaacgctac cataggggcg ggcacgcctc
     3421 tgaaaatagt ttttgaggcg ctgtcgtacc atttgtcggc gtcccgcacg gtcagcacca
     3481 ccttcgcctg cgggtattgt gccgtgaatt cccgatagaa catgcaggct gggaagtcgg
     3541 ccgtggactg gtagccgtcg aaaagaaatt cgtagttcgg cttttcacct gcgaccagtt
     3601 tttcccattc tggcaaatgc tcagggtgtt tcatcgtcag ttcgaacata tgatagcaag
     3661 cgccaaagcc gagtatctcc agggcttgct tcaatgactc ggtgccagtg cgtcccagcc
     3721 ccgcgccgat gactttcaag gattgagtgt tcattttgaa attctccttt ggcgttctta
     3781 caaaacaggg cgcgttggat aatccgattg actctcgaag tggttttcca gcgcgctcat
     3841 cactgccagc gccacatctt cgcgccaatg ccgcgccgcg acctgcacac ccacaggcaa
     3901 gcccacactg ccagtttcca cccgtaacgc cgcctgttca acgctgtctc ggctgggctg
     3961 gcggtcgctt tcctctcccg cacggactcg tcccgccgcc accacaccag cgggcatgcc
     4021 cagcacattg aagaggcggg cgtaactgtc aaagtccagc aggtcgttgg tactgccgtg
     4081 caggacggct ggtaaagatg tcggggggca aatgatcgca tcgaagccac ccgcttcgag
     4141 gtcccgcatg aagcgcaggc gatgttgatt ccgctccacg accagtttcc aatatccttc
     4201 cgcagaccgc atcccgatcg cctcaaagat tcgcaggaag cgttcttctc ccgccgactt
     4261 caagcgtttt acaaacagtt tcttcataaa cccaggcatt tgcttgctgc ggatgatgcc
     4321 cttgagttgc ggcatgggct catcgttgcg caaggcatgg cgcaatgcgg cgccatcgtc
     4381 ggacttaaac acgctgtaga acaagagtgt gagttcgttc accgcaggcg gagtccattc
     4441 gacgacctgc gcgcccatgc cgcgcaatgc ctcagccgct tctctcacca cccgacggat
     4501 ggcaggcgcg gctttgaaat aaccatcatc ttcatacaca gccacgcgca gggatttgat
     4561 atccacatcg gcaaaattgc gccacggcac aggcggaatg gacggatcga gcaacccctg
     4621 tcctggcgcc gccaacacgt ccattgccaa ggccaggtcg gcaactttgc gcgccatcgg
     4681 accaggttgt ggaatgatca cttcctgccc gcgcggcaga agatcggagc gggtatcgaa
     4741 gttggtcagt cggaacgccg ttggtttcag cccctgaatg ccgcagaaat gcgcaggctc
     4801 gcggatgctc ccgccaaagt cgccgcccaa cccgagcggt gatccgcctg ccgcgatgat
     4861 ggcagcttcg ccgccgctgc tgccaccgca actgcgttcc agattccacg ggttgttggt
     4921 tctgccatac aagggattat ccgattcgat gaacatcagc atttgggaca cattcgtctt
     4981 gccaaggatg atcgcgcccg catcgcgcaa gcgcttcacc agcggaccgt cctcttcggc
     5041 acggtggttt ttctggctgg gcaaacccat tgtggttggg gttcccttga ccagaaactg
     5101 ttctttgatt gtgataggca caccatgcaa tgcacccaat gctgcgccgc gtttttgcgc
     5161 agcatcagct tcgtccgccg ccttgcgcgc ctcatcgaac attggtacga ccactgcatt
     5221 gattttcggg ttgaccgttt caatgtgctc gatgtgtgcg tccactgctt cccgtgccga
     5281 aaattttcga gacttgatac cttgggcgat ctgggttgcg gataagtctg tgattttgtt
     5341 cattttgtca gctccttttg gtttgtgtca cctgcggacg gatccttcat ccatcgttcc
     5401 agaaaaaccg tgagttcttc caaattcttg aatccgatac gcgtctcctt ctgtgagtcc
     5461 tgcaaactga atcgccatgc c
//
LOCUS       DAOQLC010000205         5440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6760_length_5440_cov_25.343175, whole genome shotgun sequence.
ACCESSION   DAOQLC010000205 DAOQLC010000000
VERSION     DAOQLC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6760_length_5440_cov_25.343175"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PL000_20210"
     CDS             complement(<1..1072)
                     /locus_tag="PL000_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC91261.1"
                     /translation="MTEKPKAIVPVKAADSAPVQQDKSYNPKNIEKKWQDQWDADQLY
                     RSVIDPSKPKHYALTMLPYPSGDLHIGHWYAMTPSDARARYMRMKGYNVLFPMGFDAF
                     GLPAENAAIKDGVHPMKRTFQNIERMRKQMRSMGAMFDWKREAVSADPAYYKWTEWFF
                     IQLYKAGLAYRKMSAVDWCPKCNTTLAREQVWGDDRHCERCGTPVIKKDLDQWFFKAT
                     KYADELLSFEGIDWPERVKTLQTNWIDRSEGASVIFKTEQGDDVEIFTTRPDTLWGAT
                     FMVFSPEHPLVEKITTEENKATVNEYKLQAARQTDIQRGAVDKEKTGVFTGGYAINPV
                     NQARIPIWIADYVLMSYGTGAIM"
     gene            1038..1427
                     /locus_tag="PL000_20215"
     CDS             1038..1427
                     /locus_tag="PL000_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91262.1"
                     /translation="MTGTIAFGFSVTGTTAFGFSFATTTAIFWAGTIALVVFFAALGA
                     AFLTGAFFAATDFLVTAFLATGLLLTTVFFAAGLALVAAFFTTFFVFEATALAVVFFF
                     TVVFFFSAILTSSNKYQVSRTQALMDH"
     gene            complement(1834..1909)
                     /locus_tag="PL000_20220"
     tRNA            complement(1834..1909)
                     /locus_tag="PL000_20220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1874..1876),aa:Ala,seq:ggc)
     gene            complement(1932..2004)
                     /locus_tag="PL000_20225"
     tRNA            complement(1932..2004)
                     /locus_tag="PL000_20225"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1969..1971),aa:Ala,seq:cgc)
     gene            complement(2070..3380)
                     /locus_tag="PL000_20230"
     CDS             complement(2070..3380)
                     /locus_tag="PL000_20230"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC91263.1"
                     /translation="MQIGQVGLEFDLKKKYTVSMPTLSKTGQLKELEGRHSLLLRLVE
                     LSVTLNSTLDLDVLLQLITATATELLECEASSILLYDEKNPRLFFAAATGSDPAKLAE
                     IPVPIDNSLAGTIFRTNKALILNNAEHDPRHYAPVSEHIKFKTNTLLGVPMPIKDRTM
                     GVLEAVNKRNGAFNDGDVATLSVIASHAAIAINNARMLRTTQQALEKVKVTNQIKSNF
                     LALASHELRTPLGIIIGYATFLQEGAKGESSDHANQVLGAAAQMRALLDQMTNLTLLQ
                     TDEMQMRPIRISIQDVLNFAVDEIKYFAARRDLSLVFDFPETPIYVNVDAEKTALAFV
                     NLLNNAIRFSPEGSEIVIGAVEQGKKVLASVRDHGIGIPSDKLQKIFEEFYQIEPPNT
                     RRHGGLGIGLTIAKGLIEAQDGKLWAESDGLEQGSTFKVLLSTA"
     gene            3405..>5440
                     /locus_tag="PL000_20235"
     CDS             3405..>5440
                     /locus_tag="PL000_20235"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC91264.1"
                     /translation="MPILPLDPLPRTPYNLNRMSRFIRLHAPILGTLVFQGLLLFWAV
                     RTDGRDLPHIATLFSLVISLLILITGLLPAIVRPQESHTLVACFAVSFAFLLFIPFEI
                     SDMPFSQPGAPLIQFIPPLILARLINGAVILPMAIHMSARFPRRNSIPTHAIVGAYIV
                     SIIVVSLFLAASYPWQRLTMLIFTMGWFLVVIIFFFMNLLHAARDPINLQDAQRARIV
                     FFSIAVAEIPLLMRPFSIALNLNALPYNVVLFFQLFVPLGISYAVLRHDLFGIDRVLR
                     RTLAYGTVSLLLLTLYLALTTGMTALFADSITSRPIAPLVSLFIAAILFEPTRKFVQT
                     WLDRILYPDRLRFQNAVQEMQNSLARANRREQIIHLLNEVFPAQIGAEWGALKLFPEP
                     DVPPPHLTPGWNTRLIAGSVSFGGYWLGTRKAGPLYDSDERARLSALGQQAAFALAYA
                     NAYENLYALNQTLEERVKEQTQLALENQKSVAAYEERQKIARDLHDSVTQSLFGIHLM
                     ARGLAAKSTLEMREDLVALETQARETLREMRLLLNQLRNASAEENVDLTEAIQGACEA
                     FAQRSGPEGGPLLSITLEMPKGVILQKSIADEALWVIREALQNIVKHSGSRDATVTVQ
                     KDTMLRGTIRDFGKGFDVNDLPAGHYGLRGMRERVLALGGELKIEFNEGTTVSFA"
BASE COUNT         1176 a         1504 c         1374 g         1386 t
ORIGIN      
        1 ccatgatcgc gcccgtgccg taagacatca aaacataatc cgcgatccag atcggaatgc
       61 gggcttgatt aacagggttg atggcatagc cgccagtgaa cacacctgtc ttttccttgt
      121 ccactgcacc gcgctgaata tcggtctgac gggcagcctg cagcttgtat tcattgaccg
      181 tggccttgtt ctcttccgtg gtgatctttt ccaccaatgg atgttcaggc gagaagacca
      241 tgaaggttgc gccccacaac gtatcggggc gggtggtgaa gatctcgaca tcatcaccct
      301 gctcggtctt gaaaataacc gaagcgcctt cactgcgatc gatccaattg gtttgcaggg
      361 tcttcacgcg ctctggccag tcaatgcctt cgaagctcaa taactcatcg gcgtatttgg
      421 tcgccttgaa gaaccactga tccagatcct ttttgatcac gggcgtgccg cagcgttcgc
      481 agtgacggtc atcgccccac acctgctcac gagccagtgt ggtgttgcat ttcgggcacc
      541 aatccaccgc agacattttg cggtaggcca gcccagcctt atacaactga atgaagaacc
      601 attctgtcca tttgtagtag gcggggtcgg cagaaacggc ttcacgcttc caatcgaaca
      661 tcgcacccat cgaacgcatc tgcttgcgca ttcgctcaat gttttggaaa gtgcgcttca
      721 tggggtggac accgtctttg atggccgcat tttcggcggg caggccgaag gcatcaaaac
      781 ccatcgggaa cagcacgttg tatcccttca tacgcatgta acgcgcccgc gcatcagacg
      841 gggtcatcgc ataccaatga ccgatgtgca agtcaccact ggggtacggc agcatggtca
      901 acgcgtagtg tttcggcttg gaagggtcaa tcaccgaacg gtacaactga tccgcatccc
      961 attgatcctg ccatttcttc tcaatattct ttgggttgta ggatttgtcc tgctggacgg
     1021 gtgcagagtc agccgccttg actggcacaa ttgccttcgg tttttctgtc acaggcacaa
     1081 ctgcctttgg cttttccttt gcaaccacca ccgccatttt ttgggcgggc acgattgcct
     1141 tggtggtttt ctttgctgcc ttgggggcag ccttcttgac aggtgccttt tttgcagcaa
     1201 cagacttttt ggtcacggct tttttggcaa caggcttgct tttaaccacg gttttcttcg
     1261 ccgctggttt agccttggtc gcagccttct ttacaacctt ttttgttttt gaagcaaccg
     1321 ccttggcggt ggtcttcttt tttactgttg ttttcttttt ctcagccatt cttacctcat
     1381 caaataaata tcaggtttca aggacacagg cactaatgga tcattagccc tgtttgttca
     1441 ggcaattcgg aaggaacgga aggcctgcaa gcaaaggtcc ctgcttccaa aagataacta
     1501 tatttttcat atcagcctcc tttccatgat taaacaaaaa acccttcatc cacatcaggg
     1561 acgaagagtt tactccgcgg taccacccag attcgattga aagttgacat caatcgctct
     1621 ttatcgctat aacgggccta cccgtcgctg actactttat ttcaccagca aagtcctctt
     1681 gcgagataac aggcgagttc ggttctggag tgctccggtg acactggtgc tgggctttca
     1741 cctgccccaa ctcgctgacg gatatgacct actactcctg ttttgacttt ttattattgt
     1801 aggtcacgct tacaacgtga cctacgcttt tgttggtgga cccagaggga ttcgaaccct
     1861 cgaccttctc aatgccattg agacgcgctc ccaactgcgc tatgggccct tatgaatgtt
     1921 ttctgtagca gtggacctga ggggattcga acccctggcc tcctcagtgc gattgaggcg
     1981 cgctcccaac tgcgctacag gcccgttcgt aaggcgaagc gtattctacc tgaggcattt
     2041 gggaatgtca atgtggaaac gggggaaatt tatgctgtcg agagcaaaac cttgaaagtg
     2101 gaaccctgct caaggccatc tgattcggcc catagttttc catcttgggc ttcgatcagg
     2161 cctttggcaa tggtcaggcc aattcccaag ccgccatgac gacgggtatt gggcggttcg
     2221 atctgataaa actcttcgaa tatcttttgc agtttgtcag atgggatccc gatcccatgg
     2281 tcgcgcaccg acgccaaaac cttcttcccc tgttcaaccg caccgatgac gatctcactg
     2341 ccttcgggcg aaaaccgaat cgcattgttg agcaggttga caaaagccag cgccgtcttt
     2401 tcggcatcca cattgacata aatgggagtc tctggaaaat cgaagaccag gctcaggtca
     2461 cgccgtgccg cgaaatattt gatttcatcc acagcgaaat tcagcacatc ctgaatcgag
     2521 atgcggatcg ggcgcatttg catttcatcg gtttgcagca gggtcagatt ggtcatctgg
     2581 tcgagcaggg cacgcatctg tgcagcggca cccaacacct ggttggcatg gtctgaagat
     2641 tctcccttgg caccttcctg caaaaaggtg gcatacccga tgatgattcc cagcggggtg
     2701 cgcaactcat gcgaagccag cgcaaggaaa ttgctcttaa tctggttggt gactttgacc
     2761 ttctccaaag cctgctgggt ggtgcgcaac attctcgcgt tgttgatggc gatcgcagca
     2821 tgggaggcga tgaccgacaa ggtagccaca tcgccatcgt tgaaggcgcc attgcgcttg
     2881 ttgaccgcct cgagcacacc cattgtccga tctttgatgg gcatgggcac accgagcagg
     2941 gtgttcgtct taaatttaat gtgttcagaa acgggggcgt agtggcgcgg atcatgctcc
     3001 gcattgttca ggatgagtgc cttgttcgtg cggaaaatgg tgcctgccag gctgttatca
     3061 atggggacgg ggatttccgc caatttcgca ggatcagacc ccgtggctgc tgcaaaaaaa
     3121 aggcgcggat ttttttcatc gtaaagcaaa atggatgatg cttcgcactc cagcaattct
     3181 gtggcggtgg cagtgatcaa ctgaagcaga acatcaagat caagcgtgga attcagcgtg
     3241 acgcttaatt ccaccaaacg caacaacaaa ctatggcgac cttcgagttc tttaagttgg
     3301 cctgtcttcg aaagtgttgg catggagaca gtgtacttct ttttcaaatc gaattcaagc
     3361 ccaacttgtc cgatttgcat gtcaaaaaca tggcaagtgg aggtatgccc attctcccac
     3421 ttgacccact cccccgcacc ccctacaatt tgaacagaat gtcccgtttc atacgcctcc
     3481 atgcgcccat tttaggaacc cttgtatttc aggggcttct cctgttttgg gcggtgcgca
     3541 ccgacggccg agacctgccc cacatcgcca ccttgttttc gctggtcatt tcacttttga
     3601 tcctgatcac tggccttttg cctgccatcg tccgcccgca ggaaagccat acccttgtgg
     3661 catgctttgc agtttcattc gcctttctgc ttttcatccc gtttgaaatc agcgacatgc
     3721 ctttttccca accaggcgcg ccgctgatcc aatttatacc gccgttgatt cttgcgcgtc
     3781 tcatcaatgg agccgtcatt ttgccgatgg caattcacat gagtgcgcgt tttccgaggc
     3841 gcaattccat ccccacacat gcgatcgtgg gcgcgtacat tgtttcgatc attgtggtat
     3901 ccctgttttt ggcggcatcc tacccgtggc aacggttgac catgctgatc ttcacaatgg
     3961 gatggtttct ggtcgtgatc attttcttct tcatgaattt gctccatgca gccagagacc
     4021 ccatcaacct gcaagatgcc caacgcgccc gcatcgtttt cttcagcatt gctgttgcag
     4081 agattccgct tttgatgcgc cccttttcca tcgccctcaa cctcaacgcc ctgccctaca
     4141 atgtggtgtt gttcttccag ctttttgtgc ctttgggaat ttcctacgcc gtattgcgac
     4201 acgacctctt cggcattgac cgagtcctca ggcggactct ggcctatgga acggtctcgc
     4261 ttcttctctt aactttgtat ctggctctga ccacaggcat gaccgccctt tttgcagatt
     4321 cgatcacctc gcggccgatc gcgcctttgg tcagtttgtt catcgccgcc attctcttcg
     4381 agccgactcg caaattcgtc caaacctggc tggaccgaat cctctacccc gacaggctgc
     4441 gattccaaaa cgcggtgcag gagatgcaaa attcgctggc acgtgccaac cgccgcgagc
     4501 agatcatcca ccttctaaat gaagtcttcc ccgcacagat cggagcggaa tggggcgcgc
     4561 tgaaattatt ccccgaaccc gatgtgccgc ccccacacct gaccccaggc tggaacacac
     4621 gcctgatcgc agggagtgtt tcttttggcg gctactggct gggcacacgc aaagcgggtc
     4681 cgctctatga ttcggatgaa cgcgcacgct tgagcgcatt ggggcagcag gctgcttttg
     4741 ccctggcgta tgcaaatgcc tatgaaaatt tgtacgcgtt gaatcaaaca ctggaagaac
     4801 gcgtaaaaga gcagacgcaa ctcgcgcttg agaatcaaaa atcggtggcg gcgtatgaag
     4861 aacggcagaa gatcgcccgt gacctgcacg actcggtgac acaaagcctg ttcggcatcc
     4921 acctcatggc ccgcggactg gcggccaaat ccacgcttga gatgagggag gatctggttg
     4981 cgctggagac tcaggcacgt gagacactgc gggaaatgcg cctgctgctt aatcaattgc
     5041 gcaatgcttc agctgaagaa aatgtcgatc tgacagaagc cattcaaggg gcgtgcgaag
     5101 cgttcgctca gcgaagcgga cctgaaggcg ggccgctcct ttccataacg ctggaaatgc
     5161 caaagggagt catcctccaa aaaagcattg ccgatgaggc cttgtgggtg atccgcgagg
     5221 cgctgcaaaa tattgtcaaa catagcggca gccgcgacgc aaccgtgacc gtgcaaaaag
     5281 acaccatgct gcgcggaacg attcgggatt ttggaaaagg gtttgacgtg aacgacctgc
     5341 ctgcggggca ttatggcctg cgcggaatgc gcgaacgtgt gctggccctc ggcggcgaat
     5401 tgaaaataga gttcaacgaa ggtacgaccg tttcgttcgc
//
LOCUS       DAOQLC010000206         5405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6836_length_5405_cov_22.474579, whole genome shotgun sequence.
ACCESSION   DAOQLC010000206 DAOQLC010000000
VERSION     DAOQLC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_5405_cov_22.474579"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PL000_20240"
     CDS             complement(<1..368)
                     /locus_tag="PL000_20240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91265.1"
                     /translation="MSIHQFHLRRAGMEDAPAVTECVHSAFRHYIERIGREPGPMLMD
                     YAHEIHEHQVWVVEQNGQVIGALVMCIKEEGFLLDVIAVEPFHQGMGVGKLMLEFAES
                     EAQRQGFDSIYLYTHEKMTE"
     gene            480..2219
                     /locus_tag="PL000_20245"
     CDS             480..2219
                     /locus_tag="PL000_20245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91266.1"
                     /translation="MKLIREYWDLLSTYLKPQRLQVLGLGVILCAGIGLQLFTPRVLR
                     DFIDLALSGGEYDLLVRKATLFFTMVLVTQVIATAATYMSERVAWTATNLLRVDLAAH
                     CLRLDMSFHQKHSSGSLIERVEGDVDALSNFFSQFIVRVIANAVLIIGVLVMLFREDW
                     RAGLGLSIFAGLAIYAMLRVRQIGVPRWLALRQKSAEAFGFLGETLHGTEDIRANGAV
                     RYILARFDEILRAWFPLRRRAMVAFSMIWTTTLLLFTFGTGFSFGLGYVLWSAGAITA
                     GTVYLIFNYTEMMRQPIEQVRAQMEDLQRASASIERVRELFALKSSIKDGDHSLESQG
                     ALSVKFNNVMFSYGEETSALSGIDFHLEAGKVLGLLGRTGSGKSTMARLLLRLYDPNS
                     GQILLGGVPTRAAKLADLTRRVGLVPQEVQVFGATVRENFTLFDPAIPDEKILAAIHE
                     FELSPWFDSLPRGLDTELGPGGGLSAGQAQLLNFARVALRDPGLVILDEATSRLDLAT
                     EALIDRATARLLKGRTGIIIAHRLHTVQKADMILILESGAIQEIGSRHELLANPASRF
                     SALLKTGLEEVLA"
     gene            2216..3967
                     /locus_tag="PL000_20250"
     CDS             2216..3967
                     /locus_tag="PL000_20250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91267.1"
                     /translation="MKTHRILWEMIRYRPWLYTVNAVLWTLIHLAPLIPGLIIQRFFN
                     VFGAETTPASTVWALILLLIVTAVGRGGLFIGGIFVDQRHRFAMSALLRRNILAAILE
                     LPGASALKHTPGDALSRLREDANYAEDAISWTLDAIGMGSFAVVSIGILLSISTRITL
                     FVFVPLVGVIALSQMAATRIHHLRKTSREATGRVTSAISEMFDAVQAIKVAGAEDHVI
                     THYGRLGNIRRSAMLKDRLMTQLLDSVSASAVNLGTGVILLLSVSSLDAHLPLGDFAL
                     FVYYLGFVTEFTQFFGRFLAQYRQAGVSFERMIELVDGLPPERIVSHRPLDLTSTAPL
                     YLDPEPASASLQRLAARDISYRHPNSGRGLDRVNLSLVRGSFTVVTGRVASGKTTLLR
                     VLLGLLPREGGRIDWNGEPVNDPGTFFVPPRCAYIAQVPWLFSGTLRENILLGLKENP
                     TALTEAIRLSVFEQDLAQMPDGLETVIGPKGVRLSGGQVQRAAAARMFVRQPDLLVMD
                     DLSSALDVETEEKLWQRLAAMRDTTCLVVSHRRAAYRQADHIIVLKDGRVEAEGTLND
                     LLKSSPEMQSLWEQQAS"
     gene            complement(3983..>5405)
                     /locus_tag="PL000_20255"
     CDS             complement(3983..>5405)
                     /locus_tag="PL000_20255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC91268.1"
                     /translation="TPWFEASISPMGEDATLWVARDISERKQVETKLQLQSAVLEAVA
                     NTVVITDRNGLIQWTNSSFKNLTGFEPIEAIGKSPGELVKSGIHDKDFYRKLWETILS
                     GRIWQDELVNRRKDGSLYFEEMTITPLKDAQNEISHFIAVKQDITKRKKAENDLMQSE
                     QAYRTLFENMPIGLYRTLASGEILDANPALVRMFGYPDRTTLIQQKPEDLYANPDANQ
                     KFKEAIRQNGTLSGYESEFRRYDNTNFWAEDYVHVINDVWGNPLYYEGSLIDITERKR
                     AEDELRRANKNLETTHRELQQMLAHEQVLARTDGLTGLYNRRYFFELAVREFNMSARY
                     QHKLTIILFDVDGFKQANDTFGHDLGDRILAKIAQVAAAQIRDVDILARYGGDEFIIL
                     LPQTDTKQAFLVAERIRENVSTISMETGNNVPLSVTLSMGVAELQTSPQDKSIEEVIR
                     HADQALYKAKKNGRNHAYIYLDD"
BASE COUNT         1041 a         1418 c         1533 g         1413 t
ORIGIN      
        1 ttctcggtca tcttttcgtg ggtgtaaaga tagatgctgt caaatccctg ccgttgagct
       61 tcagattcag cgaattcgag catgagcttg ccgacgccca ttccctgatg gaatggttcc
      121 acggcaatga catccagcag aaaaccttct tccttgatgc acatcaccag tgcgccgatg
      181 acctgtccgt tttgctccac gacccatact tgatgttcgt ggatttcatg ggcatagtcc
      241 atcagcatgg ggccaggttc ccgaccgatg cgttcgatgt agtgtcgaaa ggcgctgtgt
      301 acacattccg tgacggcagg tgcatcttcc attccagcgc ggcgcagatg aaattgatgt
      361 atgctcacag gcattgcctc cgggtttcat tgtacggttt tcaacaagca tggtcaagca
      421 cggtgattct tgaaatcgct tatgattgaa cgaccgattt accaagagga gcataccctg
      481 tgaaattgat ccgcgagtat tgggatttgt tatccaccta cctgaagccg cagcgattgc
      541 aggtgctggg gctgggtgtg attttgtgtg cggggatcgg cttgcaattg ttcaccccgc
      601 gtgtgctgcg tgattttatc gatctggcgc tttcaggtgg agagtacgat ctgcttgtgc
      661 gcaaggcaac tttgttcttt acgatggtgc ttgtcacaca ggtgatcgcc actgccgcca
      721 catacatgag cgaacgcgtt gcatggacgg cgaccaatct cctgcgggtg gatttggcgg
      781 cacactgcct gcggttggat atgtcttttc accagaagca ttcgtcgggt agtttgatcg
      841 agcgcgtcga gggcgatgtc gatgcgcttt ccaatttctt ttcccaattc attgtgcggg
      901 tcattgccaa tgccgtgctc atcatcggcg tgctggtgat gcttttccgc gaagattggc
      961 gcgccgggtt ggggctttcc attttcgcgg ggcttgccat ttatgccatg ctgcgcgtcc
     1021 gccagatcgg tgtgccgcgc tggctggcat tgcgccagaa atcagccgag gcctttggct
     1081 ttctcggtga gaccctgcat ggcacggaag atattcgtgc caatggcgcc gtcagataca
     1141 ttttggctcg tttcgatgag atcttgcgtg catggtttcc cctgcgccgc agggccatgg
     1201 tcgctttcag catgatctgg accaccacgc ttttgctttt cacctttggc actggttttt
     1261 cttttgggct tgggtatgtg ctgtggtcgg cgggtgccat cacagcaggg acggtgtacc
     1321 tcattttcaa ttacaccgag atgatgcgcc aacccattga acaggtccgc gcccaaatgg
     1381 aagacttgca gcgtgccagc gccagcattg aacgggtgcg cgaattgttc gcgttgaaat
     1441 cgagcatcaa ggatggagac cattcgcttg aatcacaggg tgcgctgtcg gtgaaattta
     1501 acaacgtcat gttcagctat ggcgaggaaa catctgcgct aagcggaatc gattttcatt
     1561 tggaggcggg caaggtgttg ggattgttgg gccgcaccgg cagtggaaaa agcaccatgg
     1621 cgcggttgct gctccgtttg tacgacccga actccgggca gatccttttg ggcggggtgc
     1681 cgacccgtgc tgccaaactc gcagatctga cacgtcgtgt ggggttggtg ccgcaggaag
     1741 ttcaagtgtt cggcgcgacc gtgcgggaga atttcacgct cttcgaccca gccattcccg
     1801 atgaaaagat attggccgcc attcatgaat ttgagctttc accctggttc gattccctgc
     1861 cgcgtggatt ggatacggaa ctcggcccgg gcggtggact ctcggccggg caggcccagc
     1921 ttttgaattt tgcgcgcgtc gccctgcgtg acccggggct ggtcattctc gatgaagcca
     1981 cttcacgcct cgaccttgcc accgaagcct tgatcgaccg tgccaccgca cgccttctga
     2041 aaggacgcac aggcatcatc attgcacacc gcctgcatac cgtccaaaaa gcggacatga
     2101 tcctgatcct cgaaagcggc gcaattcagg aaattggatc gcgccatgaa ctgcttgcca
     2161 accctgcttc gcgtttttcg gctttgctca aaacaggttt ggaggaggtg ctggcatgaa
     2221 aacccatcgc atcctttggg agatgatccg ctaccgcccg tggctgtaca cggtcaatgc
     2281 agtcttgtgg accttgatcc accttgcgcc gttgatcccc ggcctgatca ttcaacgttt
     2341 cttcaatgtg ttcggcgctg aaacgactcc agcctccacc gtgtgggctc tcatcctttt
     2401 gttgatcgtc accgccgttg ggcgcggcgg acttttcatc ggcggtattt ttgtagacca
     2461 acgtcaccgc tttgccatga gcgcgttgct gcgccgcaat attctggcag ccattcttga
     2521 actgccgggc gcctcggccc tgaaacacac ccctggtgat gcgcttagcc gcctgcgtga
     2581 agatgccaac tatgccgaag acgccatcag ttggaccctg gatgccattg gcatgggttc
     2641 gtttgcggtc gtttccatcg gcattttgtt gagcatcagc acgcgcatca cattgttcgt
     2701 gttcgtccct cttgttgggg tcattgctct ttcgcaaatg gcggccacac gcatccatca
     2761 cttgcgtaaa acaagccgcg aggccacagg gcgggtgacc agtgccatca gcgaaatgtt
     2821 cgatgccgtg caggccatca aagtggcggg cgcagaagac catgtcatca cacattacgg
     2881 ccgcctcgga aacatccgcc gttctgccat gctcaaagac cgcctgatga cccaactgct
     2941 ggattcggtc agcgcaagcg cggttaacct cggcacgggt gtcattcttc tgctctctgt
     3001 ttcctcgctg gatgcgcacc tgcccctcgg cgattttgcg ctctttgtgt attacctcgg
     3061 ctttgtcacc gagttcacgc aattcttcgg gcgtttcctc gcgcaatatc gtcaggcagg
     3121 agtttctttt gagcggatga tcgaactggt ggacggcctg ccccccgagc ggatcgtgtc
     3181 ccaccggcct ttggatttga cttcaactgc gcctctttat ctggaccctg aacctgcttc
     3241 agcgtccttg cagagactcg cagcgagaga catttcatat cgccacccca attccgggcg
     3301 cggtttggat cgggtcaatc tatcgctggt acgcggctcg tttacagtgg taaccggcag
     3361 agtggcatca ggcaagacca ccctgttgcg tgttctattg gggttgcttc ctcgtgaggg
     3421 aggccgcatt gattggaatg gggaaccggt aaatgacccc ggtacgttct ttgtgccgcc
     3481 gcgctgcgcc tacattgctc aagtgccgtg gttgttcagc ggaaccttgc gcgaaaatat
     3541 tttgctcggc ttgaaggaaa atcccactgc attgaccgaa gcgatccgcc tttctgtttt
     3601 cgaacaggac cttgcgcaaa tgcccgatgg gttggaaacg gtcatcggcc ccaaaggggt
     3661 gcgcctctcc ggcggacagg tgcagcgtgc cgccgctgcg cgcatgttcg tgcgccagcc
     3721 agacctgctg gtcatggatg acctttccag cgcgctcgat gtggagaccg aggaaaaact
     3781 ctggcagcgg cttgcagcga tgcgtgatac cacctgtttg gtggtgtcac atcgccgggc
     3841 ggcataccgg caggcagatc acattattgt tttgaaggat ggcagagtgg aggcggaagg
     3901 gacgctcaat gacctgctaa agtcctcgcc tgaaatgcaa agtttgtggg agcagcaggc
     3961 ctcttgaagt tgttagaccc gatcaatcgt ccagataaat ataggcatgg ttgcggccat
     4021 tcttcttggc cttgtacaaa gcctgatcgg cgtgacggat gacctcttca atggatttat
     4081 cctgcggcga cgtttgcaat tccgcaacgc ccatgctgag ggtgacggac agggggacat
     4141 tattccctgt ttccatggaa atggttgaga cattttcacg gatgcgctcg gcaacaagaa
     4201 aggcctgttt ggtgtcggtt tgtggcagga ggatgatgaa ttcgtcgccg ccgtagcgtg
     4261 caagaatatc cacgtcacgg atttgggcgg cagcgacttg cgcgattttg gccaggatcc
     4321 ggtctcccag atcatggccg aaagtgtcgt tggcttgctt gaatccatca acatcgaaca
     4381 ggatgatggt cagtttgtgc tggtaacgtg ctgacatgtt gaattcacga acggcaagtt
     4441 caaagaaata acggcggttg tacaggccgg tcaggccgtc tgtgcgggcc aggacctgtt
     4501 cgtgggccag catttgctgt aattcacggt gggtggtttc gagatttttg tttgcacggc
     4561 gcaattcatc ttctgcgcgt ttgcgttcgg tgatgtcgat caggctgcct tcataataaa
     4621 gtgggtttcc ccaaacatca ttgatgacgt gaacataatc ttcagcccag aagtttgtat
     4681 tgtcgtagcg gcgaaattcg gattcgtatc ctgaaagcgt gccattctgc ctgatcgctt
     4741 ctttgaattt ttggttggcg tcagggtttg catacaggtc ttccggtttc tgttggatca
     4801 gggtggtacg gtctgggtag ccaaacatgc gcaccaatgc agggttggca tccagtattt
     4861 ctccgctcgc gagggtacga tacaggccaa tgggcatatt ctcgaacaat gtccgatagg
     4921 cctgctcgga ttgcatcagg tcattctctg ccttttttcg tttggtgata tcctgtttga
     4981 cagcaatgaa gtggctgatt tcgttttgtg cgtctttcag cggggtgatg gtcatttctt
     5041 caaaatacag gctgccgtct ttgcggcggt ttaccaactc atcctgccag attctgccgg
     5101 aaaggatcgt ttcccaaagt ttacggtaga aatccttgtc atgtattcct gatttgacca
     5161 attctcccgg gctttttccg atggcctcaa taggttcgaa gccggtcaag tttttgaaag
     5221 aggaattggt ccattggatg aggccattgc ggtcagtgat gacaacggtg ttggcaactg
     5281 cttcgagaac agcgctttgc agttgaagtt ttgtttcgac ctgttttctt tcgctgatgt
     5341 cgcgggccac ccacagggtg gcatcttcac ccatcgggga gatggaggcc tcgaaccagg
     5401 gagtt
//
LOCUS       DAOQLC010000207         5346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6963_length_5346_cov_19.526177, whole genome shotgun sequence.
ACCESSION   DAOQLC010000207 DAOQLC010000000
VERSION     DAOQLC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_5346_cov_19.526177"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..744
                     /locus_tag="PL000_20260"
     CDS             109..744
                     /locus_tag="PL000_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91269.1"
                     /translation="MSDTARSYQPKPPATTEGLDDFFASEVKEGRPQGLPEELPLGVP
                     VELAALKLGLTDRAVLKRLRRGTLKGFKTKTKYGDKWLVLAAELPEGLPQGLPGDSLA
                     TIPLIPDIEQFQEKGDSQSGVPGDSRVLVVQDQGLPPVQSPELDRLCELVADLQERLD
                     KANQQLQGATFRNGYLESKLEDKEQQILLLTDRQTKQSWWANFSAWFLGRR"
     gene            1227..2873
                     /locus_tag="PL000_20265"
     CDS             1227..2873
                     /locus_tag="PL000_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91270.1"
                     /translation="MSDEGRGLLRGCYWGETTSHGVLDVDINSQYHTDQGLKQITADF
                     ACVGINLIPYRSSESGGWHLYFCFDSDVLSKEVETKIKDYLRLRSYQIASGTLEVFPS
                     GNALRLPLQKGFAWLSADGTVRTKREQLTQNEAITTFLEDLERNKANWQEAKTRIESE
                     IYSAGAGVAGNAQDSTEWLTDEGFTGLFKPGLDWEKYQRGRQYWLTGLTSNNQRHDAI
                     LCMGHYLWYGDQAVGLQALPGRRNKERRYQLIKETLAQKHNGLSEDINKERWNEVEAD
                     IQRAVSWTSQTPLVKEYEPYKLTERLLKRLEWLHKKTGKLWSIEELEKANIDRSLNAR
                     QRIALAVAQLEAEGSEIDIAKVSRRAKACNKTVAKNRDLLCSQGGEYIAGGAGGLASP
                     PDFSGGFLDSSPSENGPDRSVVVLDPVPSAGESLDLPLAGLCGVSLFHALEESQLQQS
                     PSPSQSRPVFSETLPIGSVFSRRQALPAPGGSPPLSPGLLDLGLFRTERLRASRTSDF
                     RIICRSFCRIKCRGFFRVICHRTLVGSSPALAVGRGPPGV"
     gene            2978..3712
                     /locus_tag="PL000_20270"
     CDS             2978..3712
                     /locus_tag="PL000_20270"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC91271.1"
                     /translation="MGLFAKQRTKVSNNNRNNNSNKLVITPVFQYTYSTDGGQNMARR
                     QPIAPDELFETANRLKAEGKEVTALALLDALGGGSLSTIYKHLEVWQASKPVEVITPG
                     DELPPQVLASFTASWRLATQEAGRQVIEVKEKAKEEVAAALRQFHGALDAIRKLEQES
                     EADAQEIERLSDKLEKTETALHDLQKEAAGHKSQVEQLQKLMEKQEKTIEGLRQEAAK
                     AAELKGTVATLQAQNDKLMDRLPKSK"
     gene            4089..>5346
                     /locus_tag="PL000_20275"
     CDS             4089..>5346
                     /locus_tag="PL000_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91272.1"
                     /translation="MKQRKRWLGLAVAPFALALLAFAQPAKAADYTGDCANIPDHVTG
                     DAVVNDSACVIDTGATLIVDGTLTIDAVSVDVKSEVQAVSKVTIRTTTGELKLRKKAS
                     SQFSDILLQAPQNRIETKVLEAGNSVQVDAGLNGAEGSMRDIVIDGRIVVNKHQPFSS
                     PDIHANVLLRASGPISTKTIRTDGALGDMNIKSGGIQIEAYRTIALAEGAGEFVIGGP
                     GVNGVDGELITRSTVGGGTNSVTTIGGVNIVNGNRLSQGSIRLVDVSAIKINATQSRS
                     GFLELNAQAGKIFLPEGRLDSDSAPGQRAGIVLLLAKTLDVQAGTIISASQAQDDPGS
                     SHQVVIAAETVKYRGGEAGGLKVLADGHGVPGYFGLVQLVPYGYLTPVNTTDQHNNVN
                     QMFWTYTVNTPELAKDGALTLDGTEGN"
BASE COUNT         1485 a         1287 c         1403 g         1171 t
ORIGIN      
        1 gccacataat atatattatc ggactggttt gtatgccggg aggcttcaat ctcttgaata
       61 tagcttgaaa gctagacaga gttaccgtct agccctatac tcttccccat gagtgatacc
      121 gcccgtagtt atcagcctaa accacctgcc accaccgagg ggcttgatga cttctttgca
      181 tctgaggtga aagagggacg cccgcagggt ctcccggaag aactcccgct gggggtccct
      241 gtggaactcg cagcattaaa gcttggtttg actgatcgag cggtccttaa aaggttgcgt
      301 agaggcacac tcaaaggttt taaaacaaaa accaagtacg gtgataagtg gctagtgctt
      361 gccgcggaac tcccggaagg actcccccag ggacttcctg gtgattccct cgccacgatt
      421 cccttgattc ccgatattga gcagttccaa gaaaaagggg actcccagtc aggggtgccg
      481 ggtgactccc gggttctcgt tgtgcaagat cagggactcc cccctgtcca atcccctgaa
      541 ctagacaggc tgtgtgaact ggtggctgac ttacaggagc gccttgataa agctaaccag
      601 caactgcaag gtgctacttt caggaatgga tatttagagt caaagctaga agacaaagag
      661 cagcaaattc tgttgcttac cgatcgccaa actaagcaaa gctggtgggc caacttctcg
      721 gcctggtttc taggtcgtcg gtagtctttt tgactctgag atcatataat cgtgtgtgaa
      781 tatctgccac catatttagt gtgcgccaca aaagacatgc gagagatata aattactaaa
      841 attcaagcgg ttcagtgaac cagcactagt aagaaaacag ccccgaccga ggtcgaggct
      901 atccaatcag gttttgcttt tggtcgggcg atttcctgaa gctgctagaa gaatagcgga
      961 tagcttttgc tcggtcaaga ttgctttgca aaaaaagcaa ggagtaaaag acgagcgaag
     1021 ccgcagccgt ccttgaaagc cccactgggc ttgaggaacg cagcctaaat agaaaagtta
     1081 aatcatcgca attgccacta atggcggttt cgatagatat ctgccagctc tacccagaac
     1141 gtaagacatc gatctggaag cacccaggcc agcgccgctg gatgcaatgg acaagccccc
     1201 tgaaagattc ccaaattcta ggagtcatgt ccgacgaagg gcgtggctta cttcgcggct
     1261 gctactgggg agagaccact agccatggcg tcttggatgt tgacatcaat agccagtatc
     1321 acacagacca agggctgaag cagattactg ctgatttcgc ttgtgtggga atcaacctca
     1381 tcccttaccg aagtagtgaa tcaggcggct ggcacctgta tttttgtttt gatagcgatg
     1441 tactaagcaa agaagtagaa acaaagataa aagactactt gcggttacgt agttatcaaa
     1501 ttgcctccgg gactcttgaa gtctttccat caggcaacgc ccttagacta ccgctccaga
     1561 aaggctttgc ctggctctcc gctgatggca cggtaagaac taaaagagag cagttaaccc
     1621 aaaacgaggc aataactacc ttccttgagg acctggagcg caataaggca aactggcaag
     1681 aagccaaaac ccgcatagaa tcagagattt attccgccgg cgccggcgtt gccggcaacg
     1741 cccaggactc tacagaatgg ctaactgatg agggttttac tgggctattc aaacctggcc
     1801 tagattggga gaaatatcag cggggtaggc aatactggct cacagggcta actagcaata
     1861 accaaagaca tgacgcaata ctttgcatgg ggcactatct ctggtacgga gaccaggcag
     1921 tagggctgca agccctacca ggacgccgca acaaagagag gcgctatcag ctaatcaagg
     1981 agactctagc ccaaaagcat aacgggctgt ctgaggacat aaataaagag agatggaatg
     2041 aagtagaggc cgacatacaa agggcggttt cctggaccag ccagacccct ctagtaaaag
     2101 aatacgaacc ctacaagctc accgagagac tactaaaacg cctggagtgg ttacacaaaa
     2161 agacaggcaa gctctggagc attgaagagc tagaaaaggc gaacatagac aggagcctga
     2221 acgctaggca gagaatagcc ctggccgtgg cccagttaga agccgaaggc tcagaaatag
     2281 acatagcaaa agtatccagg cgggcaaaag cctgcaacaa gacagtagcc aagaatagag
     2341 atcttctttg cagccaagga ggcgaatata tagcgggggg tgccgggggg cttgctagtc
     2401 ctcctgactt ctctggtggt tttttagact cttccccttc cgagaatggt cctgatcggt
     2461 cggttgtagt tttagatccg gtgccttctg cgggggagtc tttagacctt ccgctagctg
     2521 gattgtgtgg ggtctccttg tttcatgccc ttgaagaaag ccaactgcaa caatcaccaa
     2581 gtcctagtca atccagaccc gttttttcag aaacccttcc aataggctct gtgttctccc
     2641 gtcgtcaggc attgcccgcg cctgggggta gtcctccttt atctccaggg ctcttagatc
     2701 ttggtctttt ccgaaccgaa cggttgagag cttctaggac ttctgatttc agaattattt
     2761 gtcgcagttt ttgcagaatt aaatgtcgcg gatttttcag agttatttgt caccgtaccc
     2821 tagtgggctc tagtcccgct ctagcagtcg gaaggggtcc gccgggggtg taatttgtag
     2881 tcgccgaacc ccagagcgag cgtcaaaacc aggataggcc accggagggg tttgcagggt
     2941 ttgagataac aggcgcgcgg gcgcggaagg tccaaaaatg gggcttttcg caaaacaacg
     3001 caccaaagtt agtaataata atcgtaataa taatagtaat aaactggtaa ttactcctgt
     3061 tttccagtac acttacagca ctgacggagg gcaaaacatg gcacgtaggc aaccaatagc
     3121 gccggatgaa cttttcgaga ccgccaatag gctaaaggcc gagggcaaag aggttactgc
     3181 cctggcactt ttggacgccc tcggcggcgg cagcttgagc acaatctaca agcaccttga
     3241 ggtttggcag gctagtaagc cagtcgaagt tattaccccc ggcgacgaat taccgcccca
     3301 ggtgctagct agctttacgg cttcatggag gctagcaacc caggaggccg ggcgtcaggt
     3361 catagaggtc aaagagaagg ctaaggaaga agtggccgca gctttgcgcc aatttcacgg
     3421 tgccctcgat gctatccgta agctggaaca agaaagcgaa gccgacgccc aggaaattga
     3481 gcgcttgtcc gacaaattgg aaaagaccga aaccgcctta catgacctgc aaaaggaagc
     3541 agccgggcac aagtcgcagg tagagcagct ccaaaagctc atggaaaaac aagaaaagac
     3601 aatagaagga ctcaggcagg aagccgccaa agctgccgaa cttaagggca cagtagctac
     3661 cctgcaggcg caaaatgaca agcttatgga ccgtcttccc aagtccaaat agccgcttga
     3721 aaacacgctt ggtgcttaca caattagttc aagaaacttc gcaaaccctg ctagcgcgag
     3781 cttggacagg cgcacccgtt ttcctttagt gttcgtaaat acaagctgca tgggcttacg
     3841 gtctaactct tagccttttg tttataaatg cttgtttgca aagcgggcaa tcaaacctct
     3901 aaatgaacga gcgttcagtc tgttatccat tctaaatttc ctacaggcaa actccaaaaa
     3961 caataatagg caaagccttt caagcaatga ctcctagcgg tagcaaccgt ttggattgtt
     4021 gctgcctgcg ttggaaatgc cgatggataa caaaataaac tccaatcaac cttgaaaaag
     4081 gaaaacatat gaaacaaaga aaacgatggc taggcttagc tgtcgcccct tttgcgttgg
     4141 cactgcttgc cttcgctcaa ccagcgaaag cggccgacta caccggcgac tgcgcaaata
     4201 tacccgacca tgttactggc gatgcagtgg tcaatgacag tgcatgtgtc attgataccg
     4261 gcgcaactct gattgttgat ggtactttga caatcgatgc cgtatctgtg gatgtgaagt
     4321 cagaagtcca agcagtcagt aaagtcacca tcagaacaac caccggcgaa ttgaagcttc
     4381 gcaagaaagc gtcaagccaa ttcagcgaca tcttgctgca agcccctcag aatcgtatcg
     4441 aaaccaaggt tctggaggct ggaaactcag tgcaggtcga tgccggtctc aacggagcgg
     4501 aaggttccat gcgagatatt gtgattgatg ggcgtattgt ggttaacaag catcagccat
     4561 tttcctcccc tgacattcat gcaaacgtgc tgttgagagc ttccggccct atcagcacaa
     4621 agaccattcg caccgacggc gccctgggtg acatgaacat aaagtcagga ggcattcaga
     4681 tagaagccta cagaacgatt gctctagcag aaggagctgg agagttcgtg ataggcggac
     4741 ctggagtaaa cggtgtagac ggagagctga taacccgaag cacggtagga ggtggaacga
     4801 actctgtaac gacgataggc ggggtcaata tcgtaaatgg aaataggttg tcgcagggaa
     4861 gcatacggct tgtcgatgtc tcagcgatca agattaacgc aacgcagtca aggtcagggt
     4921 tcttggaact taatgcgcaa gcaggcaaga tattcttgcc agaagggcgg ttggactcgg
     4981 actcggcacc agggcaaagg gcagggattg tgttgttgct tgcaaagact cttgatgtgc
     5041 aggccggcac gatcatttca gcgagccagg cgcaagacga tccaggttcg tctcatcagg
     5101 ttgtaatcgc tgcagagaca gtcaaataca gaggaggtga agctggagga ctaaaggtgt
     5161 tagcagatgg ccatggtgta ccgggctact ttggtcttgt gcagcttgtg ccatatggct
     5221 atctgactcc cgttaatacc acagatcagc acaacaacgt taaccagatg ttctggacgt
     5281 acacagtgaa tacgcctgaa ctggcgaaag atggtgctct gacgctggat ggcacggaag
     5341 gcaacg
//
LOCUS       DAOQLC010000208         5336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_6984_length_5336_cov_19.836016, whole genome shotgun sequence.
ACCESSION   DAOQLC010000208 DAOQLC010000000
VERSION     DAOQLC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_5336_cov_19.836016"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PL000_20280"
     CDS             <1..333
                     /locus_tag="PL000_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91273.1"
                     /translation="KALAAIRAMDFEIIEGLRDEIKPAHVPTLLQTWNRDLPWDVKDA
                     YAALLMDQTGGTVKEFMEDALGSPTAETRAYAICILKNDFNLFSDFLVDGGVDASKVD
                     AAIAAYRS"
     gene            364..1212
                     /locus_tag="PL000_20285"
     CDS             364..1212
                     /locus_tag="PL000_20285"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNC91274.1"
                     /translation="MTNRTISHIIEPQLVVEGAGVLLRRSVAPRASNEYDPFLLFDHF
                     AFNNPLEGPIRGFPMHPHRGIETVTYMLDGSVNHRDSLGNAGTIGAGDVQWMTSGRGI
                     LHEEMPKRGQSGNIYGFQLWVNLPAAQKMGQPRYQEVNSSTIPVVEKDGATIRLVAGE
                     VNGVRGPVTEIAASPLYMDVKLAPGATFTYPIPGGHTVLAYVFEGVGEFAGEVVESVS
                     MVVFNDDGEHIEVKSDAASSGVQFMLIAGAPFKEPIVPYGPFVMNTVEEIQQTIQELR
                     NGTFIK"
     gene            1364..1828
                     /locus_tag="PL000_20290"
     CDS             1364..1828
                     /locus_tag="PL000_20290"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC91275.1"
                     /translation="MTQVLYGPRLGREGQTRVGCSAVIFDKDRKKALLTQRTDNGRWC
                     VPGGALEAGESAAEACEREVWEETGLKVRATRLIGVYSNPDQLVVYPDGNKAFFVVLS
                     FEAEIIEGELGLSNETTAFGYYSLEEMDGLPMHGQHRQRVEDALLGGAALMK"
     gene            1908..>2315
                     /locus_tag="PL000_20295"
     CDS             1908..>2315
                     /locus_tag="PL000_20295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91276.1"
                     /translation="MLGNLSDRFYKWAKGWLIFVLFILDGFFAGFLLPLIQGLMQDDR
                     GGVQPLDLMLFATPEKIFAMIERYGEFGRPFYRNVELTVDIIYPIVYLFFFGLFISWL
                     FQRGFSANSAMRKYNIIPLGAWFFDLLENIIIVI"
     assembly_gap    2316..2415
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2471..3163)
                     /locus_tag="PL000_20300"
     CDS             complement(2471..3163)
                     /locus_tag="PL000_20300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91277.1"
                     /translation="MPTQEEIYKTEGDKYEALIAREDHQGNILKSLREITPLEHRFVL
                     DIGAGTGRLACLLAPHVSHVRAFDISEEMLRVCREKFLASGLVNWQVDVADHRQLPVD
                     DASADLVVSGWSVAYLAVWNPDSWRAELEKWLGEMKRVLRPNSFIVLFESLGTGNETP
                     IKLEHLKDYYPWLEEVGFQHKWIRTDYKFESLEEAEFLSRFFFGDELGDKVKQNNWVI
                     LPECTGVWWKKM"
     gene            3184..3660
                     /locus_tag="PL000_20305"
     CDS             3184..3660
                     /locus_tag="PL000_20305"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNC91278.1"
                     /translation="MTQLSPTAQKIQDLLNSLGYNYTVIEHTESTRTAQEAADRAGCE
                     LGQIVKSLIFRGKTSGKPILVLTSGVNRVDEKRISGYAGEPISRADADFVRAVTGYAI
                     GGVPPIGHNEKMETYLDEDFLQYGVVWAAAGTPNAIFELSLNDLQKMTTGKVAQVK"
     gene            3660..4121
                     /locus_tag="PL000_20310"
     CDS             3660..4121
                     /locus_tag="PL000_20310"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HNC91279.1"
                     /translation="MFDQIYNFLRYSENLSSSGMPTAEQLSEAAKHKVQVVINLAPHD
                     VPNAIPNEGQLVESLGIEYINIPVNWGTPTEDGLDKFMDAMDERNGKVIHVHCEANFR
                     ASAFVAMYRILRLGWKEEDAFEIMHKIWDEDAYPVWKLFIEGMVSQSGKNL"
     gene            complement(4422..>5336)
                     /locus_tag="PL000_20315"
     CDS             complement(4422..>5336)
                     /locus_tag="PL000_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91280.1"
                     /translation="TEIAMSGPASTPEAEFSGLAWYGENLIMLPQYPNIFDESGDGQL
                     FYLPKAELSLYIGGARGAPIEPRPIKLIAPNLTDQIRNYQGFESIAFSGDRVFMTIEA
                     GEGTDMMGYLISGTINPDLSTLTLDTTKLAEIPPQAKSENHSDEAILIMDDKILTFYE
                     VTGKDIVSKPVVHVFDFDLNPQGTLPIDNLEYRLTDIAMGFGNTIWGINYFFPGDEDL
                     MPSSDPILERFGTGETHAKFPQVERLIRYQYSDAGLTLVNSKRPIYLTLTEDIRNWEG
                     LVVLDTAGLIMVTDKFPRTILAFVPIPR"
BASE COUNT         1341 a         1303 c         1326 g         1266 t
ORIGIN      
        1 aaggcgcttg cggccatccg cgcaatggat tttgagatca tcgaaggtct gcgtgatgaa
       61 attaaacccg cacatgtacc cacccttttg caaacctgga accgtgacct gccgtgggac
      121 gtcaaagatg cctatgcggc cctgctgatg gaccaaactg gcggcacagt aaaagagttt
      181 atggaagatg cactcggctc ccccactgct gaaacacgcg cctacgccat ttgcattttg
      241 aaaaatgact tcaatctctt cagtgatttc cttgtagatg gcggcgtgga tgcctccaaa
      301 gtggatgcag cgattgcggc ctaccgctcg taatactttc aggctttgca tcgtccaacc
      361 cacatgacca acagaaccat ttcccacatt attgaaccac aacttgtcgt ggagggtgcg
      421 ggtgtattat tgcgccgctc cgttgccccg cgggcaagca atgaatatga cccgttctta
      481 ctttttgacc attttgcctt caacaaccca ctcgaaggac ccattcgcgg ttttcccatg
      541 cacccgcatc gcggcattga aaccgtcaca tacatgttgg atggctcggt gaaccaccgt
      601 gacagcctcg gtaacgcagg caccatcggc gcaggtgatg tgcagtggat gacgagtggg
      661 cgcgggattt tgcacgaaga aatgcccaag cgcggtcaga gcggcaacat atacggtttt
      721 caactttggg taaacctgcc tgcagcccaa aaaatgggac agccgcggta tcaggaagtg
      781 aactcgtcca ccatccctgt cgttgaaaag gatggagcaa caattcggct cgtcgctgga
      841 gaagtgaatg gcgtacgcgg ccctgtcact gaaatcgccg cttcacctct ttatatggat
      901 gtaaaactgg cccccggggc gacgttcaca taccccatcc ccggcgggca tacggtgttg
      961 gcatacgtgt tcgaaggtgt tggcgaattc gcaggcgagg tcgttgaatc ggtcagcatg
     1021 gttgtcttca acgatgacgg cgaacatatc gaagtgaaaa gcgacgcggc aagcagcggc
     1081 gtacaattca tgctcatcgc aggtgcgccg ttcaaggaac ccattgtccc ctacgggcca
     1141 ttcgtgatga acacggtcga agaaattcaa cagaccattc aagaactacg caacggaaca
     1201 tttatcaaat aaagaaaaaa tctctgagaa accctcagag attttttata gcaaaattta
     1261 gcttcgtggc tttaagccag attgcaccgc acaaattact cggctaaaat ttacacctgt
     1321 aatccattga gttcaaaatc caatctacac aaggtgaatt aacatgacac aggttctgta
     1381 cggtccacgg ctcggcaggg aaggtcaaac acgggttgga tgtagtgcgg tcattttcga
     1441 taaagaccgt aaaaaggcat tgcttaccca gcgtaccgac aatggccgtt ggtgtgtacc
     1501 tggcggcgcc ctggaagctg gagagagcgc agcagaagcc tgcgagcgcg aggtttggga
     1561 ggaaacaggc ttgaaggtcc gtgcaacgag gttgatcggt gtgtacagca atccagatca
     1621 attggtcgtg tatcccgatg ggaataaggc cttttttgtc gtcctcagtt tcgaagcaga
     1681 gatcatcgaa ggtgaattgg ggttgagcaa tgaaaccacc gccttcggct attattcttt
     1741 ggaggaaatg gacggcctgc cgatgcatgg tcaacacaga caacgcgtgg aagatgccct
     1801 gctcggcggc gcagccttga tgaagtaagg acttaagtat gacccaaaac acgtcaccac
     1861 agcaacggcg gcgtgttttt ttgttaaact ttattcacgg aggaaccatg ctaggaaatt
     1921 tgtcagatcg attttacaaa tgggcgaaag gttggctcat tttcgtgcta ttcattttgg
     1981 atgggttctt tgcgggtttt ctgctgcccc tcattcaagg attgatgcag gatgaccgag
     2041 gcggagtcca accgcttgat ttgatgctgt tcgcgacccc cgaaaagata tttgccatga
     2101 tcgaacgcta tggcgaattt ggccgtccgt tctaccgcaa tgtggaattg accgtggata
     2161 tcatttaccc gatcgtttat ttattcttct tcgggttgtt catctcatgg ttgttccagc
     2221 gcggattttc cgcaaacagc gcgatgcgca aatacaacat cattccgctg ggcgcgtggt
     2281 tttttgacct gctggaaaac atcattatcg tcatcnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnntgatt gatacacaaa aagagggccg tgatatttgt atcgcggccc
     2461 tcttttatcc ttacattttc ttccaccaca cgcctgtaca ctcaggcaga atcacccagt
     2521 tgttttgctt caccttgtca cccaactcat cgccgaagaa aaatcgcgag aggaattcgg
     2581 cttcttcaag cgattcaaac ttgtaatcgg ttcggatcca cttatgctgg aatccgactt
     2641 cctccagcca gggataataa tctttgagat gttccagctt gatcggagtt tcgttgcccg
     2701 tgcccaacga ttcaaacagg acgatgaagc tgttcggcct taagacgcgt ttcatttcac
     2761 ccagccattt ttccaactca gcccgccacg aatcgggatt ccaaactgcg agataagcca
     2821 cgctccaacc agagacaacc aggtcggcag aggcatcatc cacaggcagc tgacgatggt
     2881 cggccacatc cacctgccag ttgaccaatc cactggcgag gaatttctca cggcaaaccc
     2941 tcaacatttc ctctgaaatg tcaaacgcgc ggacatgcga aacatgcgga gcaagtaaac
     3001 aggccagccg tcccgtaccc gctccaatat ccagcacaaa gcggtgttca aggggcgtga
     3061 tctcgcgcag agatttcaga atatttccct gatgatcctc gcgggcgatc aacgcctcgt
     3121 atttatcccc ttcggttttg tagatttctt cttgtgtagg catgagccaa ttataattgg
     3181 cgaatgacac aactttctcc aactgcccaa aagatccaag accttctcaa ctcactcgga
     3241 tataactaca ccgtgatcga acacaccgag tccacgcgca cagcgcagga agctgccgat
     3301 cgggctggct gtgaactggg ccagatcgtc aaatcgctca tctttcgcgg caaaaccagc
     3361 ggaaaaccga tcctggtgct gaccagcggc gtaaaccgtg tagacgaaaa acgcatcagc
     3421 ggatatgcag gcgaacccat cagccgcgcg gatgctgatt tcgtaagagc agtgacgggg
     3481 tatgccatcg gaggcgtgcc acccatcggc cataatgaaa aaatggaaac ttatctcgat
     3541 gaggattttc tgcaatacgg ggtggtttgg gcagcggcag gcacccccaa cgccattttt
     3601 gaattatcgt tgaacgacct gcaaaaaatg accacaggaa aggttgcgca ggtgaaatga
     3661 tgtttgacca gatttacaac tttctccgtt actccgaaaa tctatcctcc agtggaatgc
     3721 cgaccgccga acagctaagt gaggcagcaa aacacaaggt tcaagtcgtc atcaaccttg
     3781 cgccgcatga tgtccccaac gcgatcccaa atgaaggtca actcgtggag tcgcttggca
     3841 ttgagtacat caacatcccc gtcaattggg gcacacccac cgaggatggg ctggataaat
     3901 tcatggatgc catggatgag cgcaacggga aagtcatcca tgtccactgt gaagcgaact
     3961 tccgcgccag tgcattcgtg gccatgtata gaattttgcg ccttggatgg aaggaagaag
     4021 atgcgttcga gatcatgcac aagatctggg atgaggacgc ctaccccgtg tggaagttgt
     4081 ttatcgaggg catggtcagt cagagcggaa aaaacttata aaagcaaaca tgaaacatcc
     4141 cgaaattaat cattcaattc tttagccgca gatttcgcga atttacaaaa aaagccgatc
     4201 cattttatag ggtcggcttt ttttataact cgtccgtatc ttgcataagt tgcaaggaat
     4261 gtctttgaaa agtatttgaa ccacggagca ctgaaataca gagtttttat tcgttttctc
     4321 tacgttcccg tggctccgtg gttaatcttt cagacttttg caaaaattcg cgactgattc
     4381 cttaggtatg aggtgaatca tgtagttgtg gagcaaaaat cctatcttgg aatcggcaca
     4441 aacgccaaaa tcgttctggg gaatttatcg gtgaccatga tcagaccagc cgtatccaaa
     4501 acaaccagac cctcccagtt acggatatct tcggtcaagg tcaggtagat gggtctcttg
     4561 ctattgacca gtgtaagccc agcatcagaa tattgatatc ggatcaaacg ttccacttgc
     4621 gggaatttgg catgggtttc acccgtaccg aatctctcca aaatcgggtc gcttgaaggc
     4681 atcaggtctt cgtcgccggg gaagaagtaa ttgatgcccc aaatggtgtt cccaaacccc
     4741 atggcgatat cggtcaggcg atattcgagg ttatcgatgg gcagggttcc ctgtggattg
     4801 aggtcaaaat cgaaaacatg cacaacaggc ttggagacga tatctttccc ggtgacctca
     4861 taaaaagtca ggattttatc gtccattatc aaaatagctt cgtcggagtg gttttcagat
     4921 tttgcctgag gggggatctc agccagtttt gttgtatcaa gtgtcagtgt ggaaagatcg
     4981 gggttgatgg tcccagagat gaggtatccc atcatgtcgg ttccttcgcc cgcttcaatg
     5041 gtcatgaaga ctcggtcacc ggagaaggcg atcgactcaa agccttgata attgcggatc
     5101 tggtcggtga ggttgggggc gatcagttta atggggcggg gttcaatggg ggcaccgcgt
     5161 gctccgccga tgtagaggct aagctccgct ttcggcaggt agaagagctg accatcacca
     5221 ctttcatcga agatattcgg gtattgaggc agcatgatga gattctcacc ataccaggcc
     5281 agcccggaga attccgcttc aggtgtggat gcaggtccgg acatggcgat ctcggt
//
LOCUS       DAOQLC010000209         5318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7026_length_5318_cov_18.535246, whole genome shotgun sequence.
ACCESSION   DAOQLC010000209 DAOQLC010000000
VERSION     DAOQLC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7026_length_5318_cov_18.535246"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PL000_20320"
     CDS             complement(<1..439)
                     /locus_tag="PL000_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91281.1"
                     /translation="MAGLAEGYLMGLKYISGLQEMEQSQARFQWEQAAQRRAQDEHQQ
                     EQSILATVFRDVRDQRTQSDTIAADGQLASKYLQAGQRLMAVNPSDGIKLINEGQKLA
                     GAADAKQLSKMEIEQMRDSYLGSIAGSIGDQSSLDEAKQKFARY"
     gene            complement(441..1718)
                     /locus_tag="PL000_20325"
     CDS             complement(441..1718)
                     /locus_tag="PL000_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91282.1"
                     /translation="MPSFGSVTGADGQVYTRTGDPNWLRNSNDHYVAASSIGGVSGGG
                     SAGGDLTYAKNSIYSPTSIPTYGPAGGVSAASLGYTALQRQLTNQEQLNQVYQPQLQS
                     YMQGDPTGGYGAQMQSLMQGQFSTNDPSYQWRLQQGQGAVERSAAARGLLNSGQAAIE
                     LMNYGQGAASTEYQAQFNRTLQAMQASETSFQSGYNRLAELAGMTPSQQIAANQANLN
                     YAKLGADTKFGYDQLAANQNLGYADLNARTGLGYAGLAERASADQAHLNLATDQFSYQ
                     AAQGQLHQDRMNNTDKAFQSMMSGYRSGGFAGGGWGAGAGASSGSGASSSYTGAMGGP
                     DAPGYGYTGALPSPFVNYDLEGSNGFFGTYSNSGPNYYYGTSTLPPSGITPYYGPNGI
                     PIYSDRPGVDPSGFGGEVSGGSFGMSGYSGGGD"
     gene            complement(1718..4192)
                     /locus_tag="PL000_20330"
     CDS             complement(1718..4192)
                     /locus_tag="PL000_20330"
                     /inference="COORDINATES: protein motif:HMM:NF022584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019073 - viral DNA genome packaging
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein"
                     /protein_id="HNC91283.1"
                     /translation="MPDIPLPFPVKYSPRDASRARGSLVKNCFEGVDAAGQPYMEKRA
                     GQILTANGAGSGQGVFTQGGQVYSIAVDKLYRNTQAAVNTGWSRISTSASYGALQTSS
                     SPIEFGGYLWFLTTTPALLKMDAAGVITTVATTGLTLNTNSSLVTLGGYMYAVVVGRN
                     DSENGIRVYRTSDGASWTSMGYDATPKGRVGYACTVFGSKIFVTGGYDSLTPSYKHAD
                     LWATSDFITWTEQNTSFFWGPTDTTYAAVSFSSYLWILGGYIPYQTAYINRSSDGHAW
                     SYLGSVPYLIQSQPIVYGGFIWALLFNVDHYQWGYTSDGLSWNFSHYVPGYASASQTI
                     PGLLSSKMFCLTVGGGNSVDVFDPALPTSSTTLISALTPSSSGKYFTFSQVPATSYNG
                     FFFKSTTDAYTYNIDTNTLTEITDADYPATTVPGFVLLDGTYYIMTPAGQIYGSALND
                     PTSWTALNVISCVAEPDGGIALARLQNYIAAFGAYTTEFFYDAGNAPPGSPLLPVQNA
                     MVEIGCAAGGSVAGIENTLLFIGVGKQMDRSVYIFNGTVPQKISTPAVDTILFADDMA
                     GVVSYCCKIKGQSFYVLTLPTTGITLVYCLDTKAWYEWTSTVAGSPRTLSAGALTYAN
                     GQATVVDPGHGYADASVILVSGASETGSNGYKIITLVDTNTYTFPTITITSGTATGTI
                     TTTGYTETYFLGSFAAGLNTNLVTQSRLTGDIQVTGESTYLDGSLPIFARLRSVGIDG
                     GTSAKKYFNALTLMAESLSSFVYLRTTKDDYQTWSAPRAVSLATERKRFNRLGAAYRQ
                     AFEILHCDNTNFHVDSILLTIDKGVD"
     gene            complement(4195..5142)
                     /locus_tag="PL000_20335"
     CDS             complement(4195..5142)
                     /locus_tag="PL000_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91284.1"
                     /translation="MATSGVTSFTMTRDSMIAASLRLLRELGAGAVPSTDDLVNCNQA
                     LNMMLKYWQVLGIPLWTYTEVVIPLVAGINKYPIGPTAAYLATAGCTITAGGSGGTPG
                     TYALGIADATGTGAAGTYTITGTAVTAINITAAGSSYSASPTLTFPLGGVIGAAATCT
                     PIGITTGLPLRPVDAFIRTIATSNDQPLTPISRTDFDDRGNKTNQGTTSEYHYDAQLG
                     NAQIYLPTTPSDSTQEIHLVIQRPLQDMVSATDNFDLPAEWFLPVKWGLADELSLEYG
                     ASDSVAARLEKRALALRTSCFDFSVEESSVFFSVDLQRR"
BASE COUNT         1297 a         1408 c         1385 g         1228 t
ORIGIN      
        1 cgtatcgtgc gaacttttgc ttcgcctcgt ccaggctgct ctgatccccg atagacccag
       61 caatcgaccc aagatacgag tcccgcatct gctcgatttc catcttagag agttgcttag
      121 cgtcggccgc ccctgcaagc ttttgccctt cgttgatcag cttgattccg tcggaagggt
      181 tgacggccat aaggcgctgc cctgcctgca ggtacttact cgcaagctgc ccgtctgctg
      241 caatggtatc ggactgcgtt ctttgatccc gaacatcccg gaagacagtg gcgaggatgc
      301 tttgctcctg ttgatgctca tcttgcgcac gacgctgggc ggcctgttcc cattgaaaac
      361 gtgcctggga ttgttccatc tcctgcaatc cagagatgta cttcaacccc atcaagtagc
      421 cttcagcaag tccggccatg ttagtctccc cctccgctat acccactcat accgaaagat
      481 cccccagaga cctccccacc aaacccactc gggtcaaccc cagggcgatc ggagtagata
      541 gggattccgt tggggccgta gtaaggggta atgccgctgg ggggtagagt ggaagttccg
      601 tagtagtaat tcggcccact gttgctgtag gttccgaaaa atccattaga cccttccaga
      661 tcgtagttca cgaaggggga tggaagagcc cctgtgtagc catatcccgg agcgtcaggt
      721 ccgcccatag cgccggtata agaactcgag gctcccgagc cactgctcgc gccagcccca
      781 gctccccaac cgccacctgc aaaaccacct gacctgtagc cgctcatcat actctggaaa
      841 gccttatcag tattgttcat tcggtcctga tgaagctgac cttgagcagc ttggtagctg
      901 aactgatcag ttgcgagatt taagtgagcc tgatctgcag aagccctttc cgccagcccc
      961 gcgtagccga gacccgtcct ggcattcaaa tccgcatagc ccaggttctg attcgctgcc
     1021 aactgatcgt acccaaactt cgtgtccgcc cctagctttg cgtagttgag gtttgcctgg
     1081 ttagctgcaa tttgctgact tggtgtcatc cccgccagct ctgcaagcct gttatagccg
     1141 gactggaagc tggtttccga agcctgcatg gcttgcaggg tgcgattgaa ctgggcttgg
     1201 tattccgtgg aagcagctcc ctgcccatag ttcatcagct caatcgctgc ttgcccggaa
     1261 ttaagcaaac cccttgccgc cgccgaccgc tctacagccc cctggccttg ttgaagcctc
     1321 cattgataac tcgggtcgtt agtggagaat tgcccctgca tgagagactg catctgcgcc
     1381 ccgtacccac cagtcggatc gccctgcata taggactgga gttgaggctg gtagacttgg
     1441 ttgagttgtt cctggttcgt aagctgccgt tgcagggcgg tgtagccgag ggacgcggca
     1501 gagaccccac cagccggacc gtaggtggga atactggtgg gggaatagat ggaattcttt
     1561 gcataggtca gatctcctcc ggcagaacca cctcccgaca caccacctat agacgaggcc
     1621 gctacgtagt gatcgttcga gttgcgcagc caattcggat cgccggtcct ggtatagacc
     1681 tgaccatccg cacctgtgac agagccaaaa gaaggcatca gtctactcct ttgtcgattg
     1741 ttaacagtat ggaatccacg tggaaatttg tattatcgca gtggaggatt tcgaatgcct
     1801 gtctgtatgc ggctcctagc cgattaaacc ttttgcgttc tgtcgccaga cttactgcac
     1861 gcggagcaga ccaagtctgg taatcatcct tcgtagttcg aagatagacg aaagatgaaa
     1921 gggactcagc cataagggta agagcgttga agtacttctt cgcagacgta ccgccgtcaa
     1981 tcccgactga acgaaggcgg gcgaaaatgg gcaaagaccc atccagatat gtgctctccc
     2041 ctgttacttg gatatcacct gtcaaacgcg attgtgtcac aaggttggta ttaagtcctg
     2101 cagcaaacga ccccaggaaa taagtttccg tatagcccgt tgttgtaatg gttccggtcg
     2161 ctgttcccga agttattgtg atggtcggga aagtataggt gttagtatca acgagcgtga
     2221 taatcttata accattactc cccgtttcgg aagcaccgga aaccaagatt acagaagcat
     2281 cagcatagcc atgacctgga tcaacaactg tcgcctgccc attggcgtag gtaagtgctc
     2341 cggcgctgag tgttctagga gaaccggcta ccgttgaagt ccattcatac caggcttttg
     2401 tatcgagaca atagactagt gtaatccccg ttgtaggtag ggtgaggaca taaaagcttt
     2461 gccctttgat cttgcaacag taacttacaa caccagccat gtcgtctgca aatagaatcg
     2521 tatcaacagc aggggtcgag attttctgcg gaactgtccc gttgaaaata taaacgctgc
     2581 gatccatttg cttccctacc ccaatgaaga gtaaggtatt ttcgatgcca gcaacagaac
     2641 ccccagccgc acagccaatc tccaccatag cattctgcac ggggagaagg ggggagccgg
     2701 gaggcgcatt gcccgcatcg tagaaaaatt ccgtcgtgta cgccccgaag gctgcgatat
     2761 agttttgcag gcgagccaga gcaattcctc catccggttc tgctacgcaa gagataacat
     2821 tcaatgcagt ccaggaagtg ggatcgttga gagctgatcc ataaatctgc ccagcagggg
     2881 tcatgatgta ataggtgcca tcaaggagaa caaatccagg aacggtagtt gcggggtagt
     2941 cggcatccgt gatctccgtc aacgtgttag tatcgatgtt ataggtatat gcgtctgtgg
     3001 tggatttaaa gaaaaagccg ttgtaggagg ttgcagggac ttgggagaat gtgaagtact
     3061 tgccggaaga cgaaggtgta agtgcgctga ttagagttgt actagaggta ggaagagccg
     3121 gatcaaaaac atccacagag tttccgccac cgacagtaag gcagaacatc ttactactta
     3181 atagacccgg aatggtttga cttgcgctgg cataccccgg aacgtagtga ctaaaattcc
     3241 aggacaagcc atctgaggta tacccccact gataatgatc gacattgaac agaagtgccc
     3301 aaataaatcc cccatagacg ataggttggg actgtattaa atacggcaca gagcccagat
     3361 acgaccaagc atgaccatcg gaagagcggt ttatgtaagc tgtctgatag ggaatgtagc
     3421 cacccagaat ccacagatag ctactgaaag aaacagctgc atacgtagtg tctgtcggtc
     3481 cccagaaaaa agacgtgttc tgttctgtcc aggtaatgaa atccgaagta gcccacaggt
     3541 cggcgtgttt gtaactaggg gtaagactat catatccacc agttacgaaa attttactgc
     3601 caaataccgt acaagcgtat cccacacgac ctttaggtgt tgcatcgtaa cccatgctgg
     3661 tccaggacgc accatcagaa gttctgtaga ctctgatccc gttttcagaa tcattacgtc
     3721 ccaccaccac ggcgtacata tacccgccga gcgtaaccag tgagctattc gtatttaggg
     3781 taaggccagt tgtcgcgaca gtggttatta cacctgctgc atccatcttg agcagagcag
     3841 gtgttgtagt caaaaaccac agataccccc caaactcaat agggctactt gaagtttgca
     3901 gagctccata agaagccgaa gtagagatac gggaccagcc agtattaacc gcagcctgcg
     3961 tattgcgata taatttatct accgcaatcg agtaaacctg cccaccttgc gtaaagactc
     4021 cctgtcccga gccagctcca tttgcggtga ggatctgccc cgctcgcttc tccatgtagg
     4081 gctggccagc agcatcaact ccctcgaagc aattctttac cagactgcct cgcgcacggg
     4141 aggcgtccct gggactatac ttaacaggaa agggcagcgg aatatccggc atggtcagcg
     4201 cctttgcaaa tctacggaga agaaaacgct cgactcttct acagaaaaat caaagcaaga
     4261 tgttcgaagc gccaaggctc ttttctctaa tctcgccgct acagaatcag atgccccata
     4321 ttcgagacta agttcatccg ccaaccccca tttcacagga aggaaccatt ccgcgggtag
     4381 gtcaaagttg tctgtcgcgg aaaccatgtc ctggagaggg cgctgaatca caagatggat
     4441 ttcttgcgta gagtccgatg gggttgtagg aaggtaaatt tgcgcattcc ctaattgggc
     4501 gtcgtagtgg tattcagacg tcgtgccctg attagtttta ttccccctgt catcgaaatc
     4561 cgttcggctt atcggggtta agggctggtc attgctagtg gcaatggttc ggatgaaggc
     4621 atcgacaggg cgcaggggaa ggcccgtcgt gataccgatt ggggtgcagg tggcggcggc
     4681 tccgataacc ccgcccaggg gaaaggtaag agttggggat gcagaatagc ttgaacccgc
     4741 tgcggtgatg ttgatcgctg ttacagctgt gcctgtaatg gtgtaagtcc ctgcagcccc
     4801 cgtgcctgtt gcatcagcaa ttcccagagc ataagtaccg ggcgttccgc ctgaacctcc
     4861 tgcagtaatg gtgcatcctg ccgtcgcaag ataagctgct gtcgggccga taggatactt
     4921 gttaatcccc gcaaccaaag gaataacaac ctccgtatag gtccacaggg gaatccccaa
     4981 aacttgccaa tacttcagca tcatgttgag agcctggttg caattcacca gatcatctgt
     5041 cgagggcaca gctcccgccc ccaactcccg cagtagacga agagacgcgg caatcatgct
     5101 atcccgcgtc atggtgaaag aagtaacgcc ggaagtagcc atgttaaagt cctagaggat
     5161 ttggaggcat aggcagggcc aaagcctccg cggtgaattg gggctctggc tgaggggtgc
     5221 cgatgacctt gggatcaggt tctctaatgt tacgcacata atcctgagga tggcggggct
     5281 cccaatgttt tgcgcaaaca tggaatccgt cccaggtc
//
LOCUS       DAOQLC010000210         5250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7158_length_5250_cov_23.345525, whole genome shotgun sequence.
ACCESSION   DAOQLC010000210 DAOQLC010000000
VERSION     DAOQLC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7158_length_5250_cov_23.345525"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PL000_20340"
     CDS             <1..768
                     /locus_tag="PL000_20340"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91285.1"
                     /translation="PFDIFERCWVLWNLSLLESHTPQETDLIQGHLDYISKRWVPKTG
                     IGFASDCAMYDSDDTSVMQRVLLRFGREADIEAVLSYEEEEYFRCVQHEASPSVGVNI
                     HILGALKAAGFGKKHPSIQKILKFLYNTRTDKGYWFDKWHVSPYYITSHVIIDCLEYD
                     LKLCQQAVDWILNTQNANGAWGYFGVATAEETAFCLQALKVWRNSGNAVPSGRIEMAH
                     MWLSENSKPPYPWMWIAKTLYYPELIIQSCIMTALTL"
     gene            799..2496
                     /locus_tag="PL000_20345"
     CDS             799..2496
                     /locus_tag="PL000_20345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91286.1"
                     /translation="MFDRLLAISANDPDDARRKKLLNILLFGLGILTIILIIVMVISL
                     LSQNAQEGTLLVVYGGIALLLGMGIIYLLNRSLPGKIAAILFLFLITSVMIFSDSPHE
                     IANGRSLFIFTIPIIMASVLLTPVSSFLFATISSVIISALAISIQEDPNSPAIAGYFL
                     VALIAWLSSRNLEQALSELRSINLNLDKVVEEKTRELASTLSRELILAGRNQAILDSI
                     ADGVIVFDEFNTAILANPALSRLADLPKDGLLHRKITDFIQNDLISPTTRGTLLGLLE
                     NPQASATGIRMDWGNKTLSTSFAQVRSSDGKNIGTVAVFRDVTHEVELEKMKNTFIGI
                     VSHELRTPLNAILGYAEMLKEGVYGPANEKQQNISLRIMTNTRRLLDIVSDLLDEVQI
                     EAGKLKIQMIACKPSDLLENLHSVMDKITSDKKLQFITELDPSMPPVLKGDPKRLQQI
                     MVNLSNNAVKFTEKGSIRVKLSRLDDQNWQIQTSDTGEGIPEEAKEYIFESFRQVKES
                     ATRQHGGVGLGLSIVKQLVELMKGKINVESEIGGGSIFTVTLPLIVPIEAETLLEKE"
     gene            2508..2873
                     /locus_tag="PL000_20350"
     CDS             2508..2873
                     /locus_tag="PL000_20350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91287.1"
                     /translation="MNEHLALIIEDDEDLSEIFGQALVAAGYTIEIIRDGAIAQERLK
                     EITPTVITLDMHIPHISGDILLNQIREDDRLTNTRVVVTTADAQMGENSRGQADLVLI
                     KPITFSQLRDLTARLKPQN"
     gene            complement(2875..4821)
                     /locus_tag="PL000_20355"
     CDS             complement(2875..4821)
                     /locus_tag="PL000_20355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC91288.1"
                     /translation="MKREVELHFEYENMSRRDDDLLRHELEERRKSLLDKVVTVQDSS
                     FSLEEHEWLARSFPVLSILAPVMSTNEGKIEFPGDPMCLYSALSYAVDQAVKTRKLGL
                     SEGAPYNDLCPQWGALPSADYRAAVDVNGIRDYHGVLLNTDQTVFDPRVWNGLVKKYF
                     VEKVLLVMRPKVVLISAVSPAHRYAIDIARVVREHLPDCLIVLGGRHVDETIHFDLVN
                     RKVVFEPSSAVVKIQEGTVEPVFDFLIGGDGYYALDVLMKAISISMDLETKSTQIDAV
                     MKTLSAIAPLFDPIPGNSVIVGMDKRKSAHAWVINSRIKLDLSVLPSPYQAFAVRALF
                     PIFQRGGRVLRTAHFMVTNSCTYHCYFCSEGVTVVGTFRSFNSDGIQRALEKVVEYIS
                     YGAEALFFDDSIFWGGNFGNVIHFCREWLHLRELAKKSPSKMIKLFGYEVEAQKVLDL
                     VWGAQFTVDLLASRRIPEEAMLALHEMKSAGCVYIYMGIESMSAPVIDKVHKNVNRKQ
                     AWDERVRAALGMARLAGITVGSSVLFGLDGETMETIEETISKVEELLVEDILSIASPN
                     ILTYHPNTAITAQHGMKDKIDYHSIGIENRPPYVYFEEAFPSVVSRNLSEEQIWFIHE
                     QATRRWGVKRNLNPMPEVILREGG"
     gene            4970..>5250
                     /locus_tag="PL000_20360"
     CDS             4970..>5250
                     /locus_tag="PL000_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91289.1"
                     /translation="MMPPRSTGMRTFTMIWFGQIISLLGSAMTWFAFTIWVWQKTEQA
                     SSLAAINTLAFLPSILLMPIAGTFVDKWERKTTLILSDLGSVTATLIALL"
BASE COUNT         1448 a         1459 c         1141 g         1202 t
ORIGIN      
        1 cccttcgata ttttcgaacg ctgttgggta ctttggaatc tttcgcttct ggaaagccac
       61 actccgcagg aaacagatct tattcagggg catctcgact acatatcaaa acgctgggtg
      121 cccaaaacag gcatcgggtt tgcttctgat tgtgccatgt acgatagtga cgacaccagt
      181 gtcatgcagc gggttttact gcgctttggc cgtgaagccg acattgaagc cgttttgagt
      241 tatgaagagg aggaatattt tcgctgcgta cagcatgagg ccagtccatc cgtcggcgtg
      301 aacattcata tcctcggcgc actaaaggcg gcagggttcg gaaaaaagca tcccagcatt
      361 caaaagattc tgaaattttt gtacaacacc cgcaccgaca aaggctattg gtttgataag
      421 tggcatgtct cgccctatta catcacttca cacgtcatca ttgactgcct tgaatacgac
      481 ctgaaactct gtcaacaagc agtggattgg attctaaaca cccagaacgc caacggtgca
      541 tggggatatt ttggtgtcgc cactgccgaa gagaccgctt tttgtttgca ggcgctcaag
      601 gtctggcgaa acagcggcaa tgcagtaccc agcggacgca ttgaaatggc ccacatgtgg
      661 ctttcagaaa actccaaacc tccatatccg tggatgtgga ttgcaaagac gttatattat
      721 ccagaactga ttattcagtc ctgtatcatg accgcgttga cgctttaaat taaatcgatc
      781 accagaaaag gcgcgaaaat gttcgataga cttcttgcca tctcagccaa tgacccagat
      841 gacgcccgca gaaaaaaact gctcaatatt ttgctgtttg gactgggcat cctcaccatc
      901 atcctgatca tcgtcatggt aatctccctc ttgagccaaa atgctcagga aggaaccctg
      961 ctggtcgttt atggggggat agccctgtta ctaggcatgg ggatcattta cctcctgaac
     1021 agatcgttac caggaaagat tgcagccatc ctgttccttt tcctgatcac aagcgtcatg
     1081 atcttcagcg acagtccgca tgaaattgcc aatgggcgca gccttttcat ttttacaatt
     1141 cccatcatta tggcaagcgt attgttgaca ccagtctcca gcttcttgtt cgcaaccatc
     1201 agcagtgtga tcatctcagc cctcgccatc tcaatacagg aagacccaaa ctcacctgcc
     1261 attgcaggat attttctagt ggccttgatc gcatggcttt cctcaagaaa cctcgaacaa
     1321 gccctgtctg aattacgctc catcaatctc aatctggata aagttgtaga ggaaaaaacc
     1381 agggaactcg ccagcacact ctcccgtgaa ttgattttgg cggggcgcaa ccaggccatt
     1441 cttgattcaa ttgccgatgg cgtgatcgtg ttcgacgaat tcaacaccgc cattctggcg
     1501 aatcctgccc ttagcaggct ggccgatcta cccaaggacg gtttgctcca ccgcaaaatc
     1561 accgacttca tccaaaacga tttgatttca cccaccaccc gcggaaccct gctcggcctg
     1621 ctagaaaacc cacaggcatc cgctacaggc atccgcatgg actggggcaa caagaccctt
     1681 tccacaagtt tcgcccaggt gcgcagttct gacggcaaaa acatcggcac cgtggccgtg
     1741 ttccgagatg tgactcatga ggtggaactc gaaaagatga aaaacacttt catcggcatc
     1801 gtttcacacg aattgcgcac tccgctcaat gccattttag gctacgcaga aatgctaaaa
     1861 gaaggcgtat acggccccgc caatgaaaaa cagcaaaaca tctcgctacg catcatgacc
     1921 aacacccgcc gcctgcttga tatcgtcagc gacttgttgg atgaagttca gatcgaagcg
     1981 ggcaaactca agatacaaat gatcgcatgt aaaccatcag atctgctgga aaacctgcac
     2041 agcgtaatgg ataagatcac atccgacaag aaactgcaat tcattaccga attggaccca
     2101 tccatgccgc ctgttcttaa gggagacccc aaacgtctgc agcagatcat ggtcaacctc
     2161 tcaaacaatg cggtcaagtt cactgagaaa ggcagcatcc gcgttaaact ttcgcggctg
     2221 gacgatcaaa actggcaaat ccaaacctca gacacaggtg aagggatccc agaagaagcc
     2281 aaggaatata tttttgaatc cttccgtcag gtgaaagaat cagccacacg tcaacacggc
     2341 ggcgttgggc tgggactttc cattgtcaaa caactggttg agttaatgaa gggcaaaatc
     2401 aatgtagaaa gtgaaattgg aggcggcagc atatttaccg ttaccctgcc cctcatcgtc
     2461 cccattgaag ccgaaacatt gctggaaaaa gaataaggag agtttccatg aatgaacatc
     2521 ttgcactgat catcgaagac gacgaagact tatctgaaat tttcgggcag gcactggtag
     2581 ctgccggata caccatcgaa atcattcgtg atggcgccat tgcgcaggaa cgtctcaaag
     2641 agatcacccc aaccgtcatc acattggaca tgcacattcc tcacatttca ggagacattc
     2701 tgttaaacca gatccgtgaa gatgaccgcc tgacaaatac acgggttgtg gtcaccactg
     2761 ccgatgcaca aatgggtgaa aactcacgcg gccaggcaga cctcgtactg atcaaaccga
     2821 tcacattttc ccaactacgt gacctgaccg cccgcctgaa acctcaaaac tgacctaccc
     2881 cccttcacgt agaatcacct ctggcatcgg attaagattc cgcttcacac cccacctgcg
     2941 ggttgcctgc tcgtgaatga accagatctg ctcttcagag agattccgcg aaaccaccga
     3001 agggaaggcc tcttcaaaat atacataggg cgggcggttt tcaatgccaa tggagtgata
     3061 atcgatctta tccttcatgc catgttgagc ggttattgcc gtattggggt ggtaggtcaa
     3121 aatgtttgga ctggcgatcg agaggatatc ttccaccagc agctcttcca ctttggaaat
     3181 ggtctcctcg atggtctcca tggtctcgcc gtccaaaccg aacaacaccg aagagccgac
     3241 tgtgatcccc gccaaccgtg ccatccccag cgcggcacgg acgcgttcat cccatgcctg
     3301 tttgcggttg acattcttat gcaccttgtc aataacaggc gcagacatgc tttcgatccc
     3361 catataaata taaacacaac ccgccgattt catttcatgc agcgccagca tggcctcctc
     3421 gggaatgcgg cgggacgcca gcaaatccac agtaaattgc gctccccaca ccaaatccag
     3481 tactttttgc gcctccacct catacccaaa aagtttgatc atcttcgagg gggatttttt
     3541 tgccaattca cgcaagtgga gccactccct gcaaaaatga atgacattcc caaaattgcc
     3601 gccccaaaaa attgaatcat cgaaaaacaa cgcctcggca ccataagaga tgtattccac
     3661 cactttctcc aatgcacgct gaatgccgtc cgaattgaac gaacggaaag ttcccaccac
     3721 cgtcacacct tcggagcaaa aataacaatg atatgtgcat gaattggtca ccatgaaatg
     3781 cgcggtccgc aaaacccgcc cgccgcgttg aaaaatgggg aacaaagctc gaacggcaaa
     3841 agcctgatag ggcgaaggca aaaccgaaag atccagcttg atgcgtgaat ttatcaccca
     3901 ggcatgggca gattttctct tatccattcc cacgatcaca gaattgcccg gtatcgggtc
     3961 gaacaaaggc gcaatagccg acaaggtctt catcaccgca tcaatctgtg tagactttgt
     4021 ctcaaggtcc atcgaaatgg agatcgcctt catcaaaaca tccagggcat aatagccatc
     4081 ccccccgatc agaaaatcaa aaacaggttc aaccgtccct tcttgaattt tcacaaccgc
     4141 actgcttggt tcaaatacga ccttccgatt gaccaggtca aaatggatgg tttcatccac
     4201 atggcggccg ccaagaacga tcaggcaatc gggcaggtgt tcacgcacaa cacgggcaat
     4261 atcgatcgca tagcggtgag cgggggaaac cgcgctgatc aataccacct ttgggcgcat
     4321 caccagcaaa accttttcta caaaatactt cttcaccaat ccattccaaa cacgcgggtc
     4381 gaacacggtt tgatcggtgt ttagcaaaac gccgtggtaa tcacggatgc cattcacatc
     4441 cacagcagcg cgataatctg ccgagggcaa cgccccccac tgcgggcaca agtcgttata
     4501 cggcgcaccc tccgaaagcc ccaacttgcg ggtcttcacc gcctgatcca ccgcatagct
     4561 caatgcggaa tataaacaca tcgggtcgcc aggaaattcg atcttcccct catttgtaga
     4621 catcacaggc gcaaggattg aaagcacagg gaaggagcgc gccagccatt catgttcttc
     4681 aaggctgaaa ctggaatcct gcaccgtcac gaccttatcc agcagagact ttctgcgttc
     4741 ttcaagttca tgccgcaaca ggtcgtcatc gcgcctgctc atattttcat attcaaaatg
     4801 caattccact tctcgcttca tggctttacc tttcataaag aggtcaacat gggcaaatcc
     4861 cttacaacga atacaccaat aataaatgcg aaccacatag ggcatcctta caaatctgaa
     4921 taggagtgta atccaaaatc ctgttatttt cctgtaagat tgagcgcata tgatgccacc
     4981 ccgctcaact ggaatgcgca catttaccat gatctggttc ggtcagatca tatcactgct
     5041 aggcagcgcc atgacatggt ttgcattcac catctgggtc tggcaaaaaa cagaacaagc
     5101 ttcatccctg gccgccatca acaccctggc atttctgcca tccattctac tcatgcccat
     5161 cgcaggcacc ttcgtagata aatgggaacg caaaaccacc ctgatcctca gtgatcttgg
     5221 cagtgtaacc gccacactga ttgccctgct
//
LOCUS       DAOQLC010000211         5226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7217_length_5226_cov_25.422742, whole genome shotgun sequence.
ACCESSION   DAOQLC010000211 DAOQLC010000000
VERSION     DAOQLC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7217_length_5226_cov_25.422742"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="PL000_20365"
     CDS             complement(<1..1782)
                     /locus_tag="PL000_20365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91290.1"
                     /translation="MGFFAGLNDEKYDRQYSDRELTRRIAGFFKTKARQLITASVLVI
                     TLAFIGAALPVVVSRMVDLLREQPTIQEIVLVGVTLMGVGVGLWGLNWMRRSLIVRAV
                     GDVVLDLRTRAFRAAAEHDLSFYDQFSSGRIVSRITSDTNDFGQLIVIITDVVSQLMQ
                     AFILGVVLFRTELKLSLLLIAFMPMIFAVAAGFRALARRVTKRGMKAMADVNAAIKET
                     ISGISIAKNFRQEQSIFASFDESNQQSYQVNVRRGFVLALVFPTLNALSGVFVGILIY
                     VGGMSAEQGLVSIGAWYLFIMSLDQFFFPILNLTSFWAQIQGGLSAAERVFALIDADP
                     NVIQKEKQDVPRLKGEIRFENFDFSYSDKEPILRNFNLAIQPGETLALVGHTGAGKSS
                     IAKLIARFYEYQNGELRIDGRDIRTFDLTQYRRQLGIVSQVPFLFSGTVIDNIRYAAP
                     GVPESEMLKLAKKIGDGEWLETLPDGLQTEVGERGNRLSMGQRQLVALMRVLVQNPAI
                     FILDEATASIDPFTEWQIQQALNLILKNTTSILIAHRLSTVKAADRIVVMQKGSIIEQ
                     GDHDGLLKQGGHYAELYNTYFRHQSLSY"
     gene            complement(1888..3651)
                     /locus_tag="PL000_20370"
     CDS             complement(1888..3651)
                     /locus_tag="PL000_20370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91291.1"
                     /translation="MSSISLPAEFTLHRKNSYNRATTLKWIASHARPYWWIVIMIIIG
                     AIGNAALAAYVPVLTGDAFNSMIESPPDTSVLLPLALIIGISQIVRGVLQLGRNFGAE
                     LMAQRMERDIRDELYLSLLGKSMTFHNLQPVGDTMARATNDVREVNFMFNPGVNLVVG
                     SFMFIIMLFFAAPRYHPSLLITPTIFTIIYFIGLARYLKSLAPITDEVRATFGDMNTH
                     LSESLDGVEIMKGAAQEGAEVDRFVSNARKVRDAFVRQGDLEGRYIAMLLLGTAYAGG
                     LVHALLLFRAGTIDLGAVVAYFGLLRLLEFPTFTSTWAYSQISSGLSSARRILELINR
                     ETHLDQNAAGRATEMRGEVEFRGVSFAYPEGDAVIQDVSFKIKQGQTVAIVGQTGAGK
                     TSLARLINRTYDVTAGQVLIDGVDVREWNLAALRSQISIIEQDIFLFSRSLSDNIAFG
                     KPGATKEEIISASIASQAHDFILDFDQTYGTVVGERGVTLSGGQRQRIALARAFLTDP
                     RILVLDDSTSAIDSATEDKIQRAIANAARGRTTFIITHRLSQIRWADLIIVLRKGKIA
                     AIGSHDELMKTSEAYSRIFRE"
     gene            3764..3913
                     /locus_tag="PL000_20375"
     CDS             3764..3913
                     /locus_tag="PL000_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91292.1"
                     /translation="MKMPKTISGWCTILFFLFTGLGAFVAIPLLPTLVGISALGVVVF
                     TLIGK"
     gene            complement(4038..5018)
                     /gene="pyrB"
                     /locus_tag="PL000_20380"
     CDS             complement(4038..5018)
                     /gene="pyrB"
                     /locus_tag="PL000_20380"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HNC91293.1"
                     /translation="MPTQSSSPHLPFGENKQADWYGKDILSVKQFSRDDLEYVFGVAH
                     EMRGMVERVGTFDLLKGKILANLFYEPSTRTSSSFTAAMERLGGSVIPISEVKYSSVS
                     KGESLPDTIRTLECYADVIVLRHPETGSAAIAAKAARKPVINAGDGVGEHPTQALLDT
                     FTIMEEIGRLDNLTVTMLGDLKYGRTVHSLARLLSTFTNIKLNYVSPDILKMPKEVME
                     EVGAKNIPQAEFSSLEKVLPETDVLYVTRVQKERFEDPADYEKVKGAYVIDPEIMKAA
                     KKDMIVMHPLPRVGEISTDFDDDPRAAYFRQMEYGLYVRMALLALVLGKA"
BASE COUNT         1251 a         1365 c         1391 g         1219 t
ORIGIN      
        1 ataagaaaga ctctgatgcc tgaaataagt attgtacaac tcggcgtaat ggccgccttg
       61 cttcaataag ccatcgtggt cgccctgctc aataatggaa cctttctgca tgacgacgat
      121 acggtctgcg gctttgacgg tggagagacg atgcgcgatg aggatactgg tggtgttttt
      181 caagatgaga ttcaacgcct gctggatctg ccattcagtg aatggatcaa tgctggcagt
      241 ggcttcatca agaatgaaaa tggcggggtt ttgcacgagc acacgcatca acgccaccag
      301 ttgacgctgc cccatggaaa ggcggttacc gcgttcaccc acttcggttt gcaggccgtc
      361 gggcagggtt tcgagccatt caccgtcacc gatctttttg gcgagtttga gcatttcgct
      421 ttcggggacg cccggcgcgg cgtagcggat gttgtcaatc actgtgcctg aaaacaggaa
      481 agggacttgc gaaacgattc ccaactgacg gcggtattgg gtcaggtcaa aggtgcgaat
      541 gtcacggcca tcgatgcgca gttctccgtt ttggtattcg taaaaacgcg cgatcaattt
      601 tgcaatggat gatttgcccg cgccagtgtg ccctaccagg gcgagagtct ctccgggctg
      661 tatagccaga ttgaagttac gcaggatcgg ttctttatcc gaatagctga aatcaaaatt
      721 ttcaaatcgg atctcgcctt tgaggcgcgg cacatcctgc ttttcctttt ggatcacgtt
      781 cgggtcggca tcgatcaagg caaagacacg ttcggcagcc gaaaggccgc cctggatttg
      841 cgcccaaaac gaagtgagat tcaagatcgg gaaaaagaac tggtcaaggc tcatgatgaa
      901 gagataccac gcgccgatgc tcaccagtcc ctgctccgcg ctcatgccgc cgacataaat
      961 gagaatgccg acgaaaacac cagacaaggc attgagcgtt gggaaaacca gcgccagcac
     1021 aaatccgcgc cgcacgttga cttgatacga ctgctgattc gattcatcga atgaagcaaa
     1081 aatgctttgc tcctgcctga aattctttgc aatggaaatg ccgctgatgg tttccttgat
     1141 cgcggcgttg acatctgcca tggctttcat gccgcgtttg gtcacacggc gggccagtgc
     1201 gcgaaaccct gccgccaccg caaagatcat gggcatgaat gcgatcaaaa gcaaagacag
     1261 tttcaactca gtgcgaaaca aaaccacgcc aagaatgaag gcctgcatca actgcgagac
     1321 cacatcggtg atgatgacaa taagctggcc gaaatcattc gtatcggatg tgatgcgcga
     1381 gacaatgcgc cccgatgaaa attgatcgta aaacgagagg tcgtgttcgg cggcggcacg
     1441 gaaggcgcgt gtacgcaggt cgagaacgac atcgcccacg gcgcgcacga tcagactccg
     1501 ccgcatccag ttcagtcccc acaagccgac tccaaccccc atcagtgtga ctcccaccag
     1561 gacgatctcc tgaatcgtcg gctgctcacg gagcaggtcc accatacggc tgaccaccac
     1621 tggcaatgcc gcgccgatga aggccagtgt aatgaccaat accgaggcgg taattagttg
     1681 tctggctttg gttttgaaaa accctgcaat gcgacgcgtg agttcacggt cgctgtattg
     1741 tctatcgtat ttttcgtcgt ttagtccggc aaagaaaccc atttttgatt tccgattttt
     1801 tgatattagg cttgtgatta atggagtacg gacttcagtc cgcttttcta caggagtgga
     1861 ctaaagtccg cattcctggg ttgggaatta ttctctgaaa atccttgagt acgcttcgga
     1921 ggtcttcatg agttcatcgt gtgaaccgat ggcagcgatc ttgcccttgc gcaggacgat
     1981 gatgagatcg gcccagcgga tctgcgacag gcggtgcgtg atgatgaagg tcgtccgtcc
     2041 gcgggcggca ttggcgatgg cgcgttggat tttatcttcg gtggctgagt cgatcgcgga
     2101 tgtggaatca tcgagcacga gaatgcgcgg gtcggtgaga aaggcccggg caagtgcgat
     2161 gcgttgacgt tgtccgccgg agagggtcac accgcgttcc ccgacgacgg ttccgtaggt
     2221 ctggtcaaag tccagaataa aatcatgcgc ctgcgacgcg atggaagctg aaatgatctc
     2281 ttccttggtg gcgccaggtt tgccaaaggc gatgttatcg ctcagcgaac gtgagaataa
     2341 aaagatatcc tgctcgatga tggaaatctg cgaacgcaaa gcagccagat tccattcgcg
     2401 cacatccacg ccatcgatca agacctgacc cgctgtgaca tcgtaagtgc ggttgatcag
     2461 cctcgcaagc gaggttttcc ctgcacccgt ctgccccaca atggcgacgg tttgtccctg
     2521 cttgatcttg aatgagacgt cttgaatgac agcgtcgcct tcagggtagg caaaggaaac
     2581 gccgcggaat tccacttctc cgcgcatttc ggttgcgcga cccgcggcat tttggtcaag
     2641 atgtgtttcg cggttgatga gttccaaaat acgacgcgcg ctggaaagcc cggaggagat
     2701 ctgtgaatag gcccaggtgg atgtaaaggt ggggaattca agtaaacgca acagaccaaa
     2761 atacgcaacg actgcgccaa ggtcgatcgt ccctgcacgg aaaagcagca acgcgtggac
     2821 aagtccgcct gcgtaggcgg tgcccagcaa cagcatggca atgtagcgtc cttcgaggtc
     2881 accctgacgg acgaaggcat cgcgcacctt gcgcgcattg gacacaaaac ggtccacttc
     2941 tgcgccttcc tgcgcggcgc ctttcatgat ctccacaccg tccagcgatt cggagagatg
     3001 tgtgttcatg tctccgaagg tggcgcggac ctcatccgtg atcggtgcca gcgacttgag
     3061 ataacgcgca agtccgatga aataaatgat cgtgaagatc gtcggcgtaa taagcaggga
     3121 agggtgatac cgcggcgcgg caaagaacag catgatgatg aacatgaagg agcccaccac
     3181 caggttcacg ccggggttga acatgaaatt aacttcgcgc acatcgttgg tggcgcgtgc
     3241 catcgtatca ccgacgggtt gaaggttatg gaaggtcata ctcttgccaa gcagggagag
     3301 gtataactcg tcgcgaatat cacgttccat gcgctgggcc atgagttcgg cgccgaagtt
     3361 gcgtcccaac tgcagcacac cgcggacgat ctgcgaaatg ccgatgatca aagccaacgg
     3421 cagcaaaacc gatgtatccg gcggtgattc gatcattgaa ttgaaggcat cccctgtcag
     3481 cacaggcacg taggcagcca atgcagcgtt gccgatcgct ccaatgatga tcatgatcac
     3541 gatccaccaa taagggcggg catgcgaggc gatccatttg agggtggtcg cccgattata
     3601 ggaattcttg cgatgcaggg taaattctgc gggaagggaa atggaagaca tgggttgatt
     3661 gtaccatttt gaaataaatg gcttttcgaa ccctttaatt cagacaggtg ttgtcttatt
     3721 agagtgacaa tcctgtcacc acacaacaca aggagaattt aatatgaaaa tgcccaaaac
     3781 gatctctggc tggtgtacga tcctgttctt tctttttacg ggtctgggag cattcgttgc
     3841 catccccctt cttcccacgc ttgtcggcat ttccgcactt ggcgtggtcg ttttcacatt
     3901 gatcggaaaa tagcccaatt ttctttttat cgttaacagg ttcgcaagtt ggaaagttga
     3961 aacgttaaac aactggaagg ttggcagcaa tcaaacctgc caaccttcca acctgtaacc
     4021 tgcagcttaa accgtactta cgccttcccc aacaccagcg ccaacaaagc cattctcacg
     4081 tacaaaccat attccatctg gcggaaatat gcagcgcgtg gatcatcgtc aaagtctgtg
     4141 ctgatctcac ccacacgcgg cagcggatgc atcacgatca tgtctttctt tgccgccttc
     4201 atgatctcgg ggtcgatcac atatgcacct ttcacctttt cataatctgc ggggtcttcg
     4261 aaacgttcct tttgcacacg ggtcacatag agcacgtcgg tttcggggag gactttttcc
     4321 agtgacgaaa actcagcttg cgggatgttt ttcgcgccga cttcctccat cacttccttc
     4381 ggcatcttca aaatatcagg cgagacataa ttcaatttga tattcgtgaa ggtcgaaagc
     4441 aaccgtgcca gcgagtggac cgtgcgtcca tacttcagat cgccgagcat ggttacggtc
     4501 aggttatcaa ggcggccgat ctcttccatg atggtgaagg tatcaagcag cgcctgagtc
     4561 ggatgctcgc caacgccatc gcccgcgttg atgacaggct tcctggcggc cttcgccgca
     4621 atcgccgccg agcctgtttc agggtggcgc agaacgatca catcggcata acattccaaa
     4681 gtgcggatcg tatcgggcag gctctcaccc tttgacacgg acgaatactt cacctcacta
     4741 atcggaatga ccgatccgcc gaggcgttcc atcgccgccg tgaacgatga cgaagtacgc
     4801 gtggagggtt cataaaatag attcgctaaa atctttccct tcaacagatc gaatgtccca
     4861 acacgttcga ccattccgcg catttcatgt gccacgccga acacatactc cagatcgtcg
     4921 cggctgaatt gtttgacgga taaaatgtcc ttgccatacc agtcggcttg tttgttttcg
     4981 ccgaaaggca ggtgagggga tgatgattga gttggcattt gtttagtctc ctggtctttt
     5041 agtttgctag tcttccagtc cttctgcttc gaccagataa ccagccgacc agaagactat
     5101 tagactaatt ttttaatcgg gaatctggtt gcacactgac aacggctccc gttttatcac
     5161 attttctacg cctcggcaaa aatccccgcg actcattcca cgggtgaggc tggttctgtt
     5221 tcgacg
//
LOCUS       DAOQLC010000212         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7289_length_5192_cov_24.091104, whole genome shotgun sequence.
ACCESSION   DAOQLC010000212 DAOQLC010000000
VERSION     DAOQLC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7289_length_5192_cov_24.091104"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PL000_20385"
     CDS             <1..222
                     /locus_tag="PL000_20385"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HNC91294.1"
                     /translation="AFFPVFPYFFWSGATAIYISLVVSAVGLFLIGAAITLMTGRSVW
                     FSGTRQVVVGIAAAALTFGIGKLIGVSLG"
     gene            304..1608
                     /locus_tag="PL000_20390"
     CDS             304..1608
                     /locus_tag="PL000_20390"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC91295.1"
                     /translation="MMTQNPRIFTGLQMDTVIKNITLIDGTSRAPIADAVIVIGEGKI
                     IYAGPASGWEDAGVESKVWDLRGRFVLPGLIDAHVHLSGSGEVDSQFAVPTGPMALKI
                     LSNAQKNLAAGITTVRDVGGWSELEFDVRRAIQRGDFAGPRMCLAGRFISITEAGADY
                     YAGMYRVADGVDEVRKAVREQVKNGADVIKMGVTGAVLVESGEPGATHFNFDEMQALV
                     QEAKKFGKRVAMHAHGIDGIRKAVEAGVDTLEHGTYLYQDPNVIQHMAERGIFLLPTL
                     KVGWDIILAEKPNIPDWIMDKNKASQDDALKSLKMAYEAGVPIAMGSDVGTPLNYHGE
                     NALEIHWMHEAGLSTMDAIIAGTGNAARALGWDSWLGTLEAGKVADLIIHEKNPLENL
                     HILADKESLRFVLKDGLIVASHLEDENSAPKELFAQNLLRVG"
     gene            complement(1615..2487)
                     /locus_tag="PL000_20395"
     CDS             complement(1615..2487)
                     /locus_tag="PL000_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91296.1"
                     /translation="MKKNLSLFLLLVLTACSSVTPVPPTVTPTATATAIPTHTPLPTA
                     TLTSTPVPSGACESPLLPLSTGNQWTYRVTTDDGEATYALKTLERDDGRNTVIKVEFT
                     DQKNNVSVVEPVVCIDSAIENFPLFVVSMYFSGYLEKDFNTYHDSGTYAPNYDILDFN
                     HWSMHWDMYYLTEDTAQLINPTDGQSLFVLQSSFIEMSFTLDGTREAVTVPAGDFPNA
                     LKAGYTFSFPVTIVLQDAMTGGTLTVKTTQWYEPYVGLVRAKVDSTSLRFYGQSVPMQ
                     LNSLIELVEFERGE"
     gene            2587..3033
                     /locus_tag="PL000_20400"
     CDS             2587..3033
                     /locus_tag="PL000_20400"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HNC91297.1"
                     /translation="MNFSPITDDLFIGTTPSSEDYNHLRGLGVQLVINMRVEYRPRKD
                     PHPTPLELLWLPTFDSPLVPIPMRALHRGANAALETIQNGGKVYAHCAGGVHRGVTMG
                     ACILIAQGMEPLPAMDLIKTKRPLADPFAFYIKPRILKFANQWKSS"
     gene            3259..>5192
                     /locus_tag="PL000_20405"
     CDS             3259..>5192
                     /locus_tag="PL000_20405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC91298.1"
                     /translation="MHPKPLYDSSLTLVRDENGHSHDLLLALSRAAQSIQRARTAEDF
                     YRAVGNEVKSLGGETTLLLINQHSPTLSIAYTSYSPSLIRKAEKITGLSLHEYQIPFS
                     AETVYGRAIHNRKAVFVESVKEAMLEIVPSPISRWIDSLISMFKLGHGILAPLLVDDE
                     TLGLLKINGTFLNQSDIPAMEVFAAQIATGLSNIRLVQKLQDELYARQKAEESLHRSR
                     KTLLALGQAAQSIQKTHTPEDIYRVVGEQIKALGLEATILTLNSNRQTLCHSYTTLST
                     DIIQNAEKMAKVSAQGYCWQIDPNGTYNRIIFSGKSEFIHKSEDLFAEALPKFMWPMT
                     KALMKILKIGQGIIAPLRVDEEDLGLLVVFGNEAISEEDCPAIDSFAGQVAISLRNAH
                     LAKKVEAEFLEKKQAEEAVRRAKEHFEALIEKAPDGITLLGMDGKVKYASPSAKRLFD
                     YSEEDITNLTPMEFIHPEDLDLVLETMNKLVQNPAYTPTIQYRYMRKDGVYHWVESTF
                     SNLLTDPNVEAVVINFHDITQRKLVEKALFESEKYYRALIENASDGILVVNEEGKISY
                     ESPSVTRMLGYEPYSLLGTNAFELIHPDDMAAIIEAFTQGSSTPNLVHRGEYRLIRKN
                     GEWGYFEIVSHYLLTDPAIKG"
BASE COUNT         1329 a         1381 c         1318 g         1164 t
ORIGIN      
        1 gcgttcttcc ccgtcttccc gtatttcttt tggagcggtg caactgcgat ctacatcagc
       61 cttgtcgtga gtgcggtcgg cctcttcctc atcggcgcgg ccatcacctt gatgacggga
      121 cgcagcgtct ggttctctgg cacaaggcag gtggtcgtcg gcatcgccgc agcagcgctg
      181 accttcggca tcggcaagtt gatcggtgtc tctttgggat aacgccctct gtcccgtaag
      241 cgggacatct cccccatttg aagaacacaa atgggggaga attttttatt tcggggatag
      301 aatatgatga ctcaaaatcc acgcatcttt acaggacttc aaatggatac tgttatcaag
      361 aacattaccc tcatcgacgg aacaagccgc gcgccgatcg cggatgcggt cattgtcatc
      421 ggggagggga agatcatcta cgctgggcca gcttccgggt gggaagatgc gggggttgaa
      481 tccaaagtat gggatttgcg cggacgattc gtcctgccgg ggttgatcga tgcgcatgtc
      541 catctttcag gctctggcga agtggatagt caatttgccg tgccgacggg gccgatggcg
      601 ttgaagatcc tttcgaacgc gcagaagaat ctcgcggcag ggatcacaac cgtacgcgat
      661 gtgggcgggt ggagcgaact ggagttcgat gtgcggcgcg ccatccaacg cggagacttt
      721 gcagggccgc gcatgtgtct ggcgggtcga ttcatttcga tcaccgaagc aggcgcagat
      781 tattacgcag gcatgtaccg cgtggcagat ggtgtggatg aagtccgcaa ggcggtgcgc
      841 gaacaggtga agaacggcgc ggatgtgatc aagatgggtg tgacgggcgc ggtgttggtg
      901 gaaagcggcg aacccggtgc cacccatttc aacttcgatg aaatgcaggc gctggtgcag
      961 gaggcaaaga agttcggcaa acgtgttgcc atgcacgcac atggcattga tgggattaga
     1021 aaggccgtgg aagctggtgt ggatacgttg gaacatggca catacctgta ccaggacccg
     1081 aacgtgattc aacacatggc tgagcgcggc atcttcctcc tgccgacctt gaaagtgggc
     1141 tgggatatca tcctcgccga aaagccgaac atccccgatt ggatcatgga caagaacaag
     1201 gcatcgcagg atgacgcgct aaaaagtttg aagatggcct acgaagcagg cgtgcccatc
     1261 gcaatgggct cagatgtcgg cacgccgttg aactatcacg gtgagaacgc gctcgaaatc
     1321 cattggatgc atgaggcggg actctccaca atggatgcga tcatcgctgg gacagggaac
     1381 gccgcccgcg ccctcggctg ggactcgtgg cttggcaccc tcgaagctgg caaagttgca
     1441 gacctgatca tccacgaaaa gaatccgctg gagaatctgc acatccttgc agataaagag
     1501 agtctgcgct tcgtgttgaa ggatgggttg atcgtcgctt cgcacctgga agatgaaaat
     1561 tccgcgccca aggaattgtt cgcgcagaat ttgttgaggg tgggttaagt tttcctactc
     1621 tcctctttca aactccacca actcgatcag gctgttcaac tgcatcggca cactttgtcc
     1681 ataaaaccga agggaggtcg aatccacctt cgcgcggaca agacccacat acggctcgta
     1741 ccattgggtc gtttttacgg tcagcgtgcc gccagtcatt gcatcctgca gaacgatcgt
     1801 cacgggaaat gaaaatgtat accccgcctt caacgcattt ggaaaatccc cagcgggcac
     1861 ggtcaccgct tcacgagtcc catccaacgt gaaggacatt tcaatgaacg aagactgcaa
     1921 cacgaacaga gattggccat ccgttgggtt gatgagttgg gccgtatctt cggtaagata
     1981 atacatatcc caatgcattg accagtggtt gaaatcgagg atgtcgtaat tgggggcata
     2041 ggtacctgaa tcgtgataag tattgaaatc cttttccaaa tatcctgaaa aatacatgct
     2101 caccacgaac agcggaaagt tctcaatggc cgagtcaatg caaaccacag gctcaaccac
     2161 agacacgtta tttttctggt cggtgaattc aacttttatc acggtgttgc gtccgtcgtc
     2221 ccgttccagc gttttcaagg cgtaggtcgc ttctccatca tccgttgtaa cgcggtaagt
     2281 ccactgattg cctgtgctca atggcagaag cgggctttca catgcaccgc ttgggacggg
     2341 cgtacttgtc aatgtggcgg tcggcaaagg ggtatgggtt ggaatcgcag tggcagtcgc
     2401 ggtgggggtc acggtcgggg gcacaggtgt aacggaggaa caggcagtaa gaaccagtaa
     2461 aagaaacagg ctcagattct ttttcatggc aagttctcct tacaatcatt ataggccgcg
     2521 cgctccagca ttgcatgtga caaagaacat atctcaggcg ccagtccttg tgataccatt
     2581 tcccgcatga acttctcccc catcaccgat gacctgttca tcggcacaac cccttcatca
     2641 gaagactaca accatctgcg cggactcgga gtccaattgg tgatcaacat gcgtgtggaa
     2701 taccgcccga ggaaagaccc gcatccgact cccctcgaac tgctctggct tccaacattt
     2761 gattctcccc ttgtgcccat ccccatgcgt gccctgcatc gcggagccaa tgccgcactc
     2821 gaaacgatcc aaaacggcgg caaggtctat gcccattgcg caggcggagt ccaccgcggg
     2881 gtgacaatgg gtgcctgcat tctcatcgcc cagggaatgg aaccattgcc agcaatggat
     2941 ctgatcaaga caaaacgccc actggctgac ccgttcgcgt tctacatcaa accgcgaatc
     3001 ctgaaattcg caaatcaatg gaaatcatcc tgacccgcaa gtcatcatca acacacataa
     3061 atacttgacg atgaaggtta actcctttat attccaataa acgccgccca tcaaaagttt
     3121 gattcccttt ttagtaattt ttcatcattt tttcttatac acggcggata tatcttaaac
     3181 ctgattcgcc tgaaccagat ccatctgttc agctgcccac accttgtcac ataaagcgaa
     3241 tctgcaagga aggtttcgat gcatccaaag cctctctatg attcctccct cacattggtg
     3301 cgggatgaaa atggtcatag ccacgatctt ttattggctt taagccgtgc agcgcaatcc
     3361 atccaacggg cgcgcacggc tgaagatttc tatcgtgccg ttggaaatga agttaagtct
     3421 ctggggggag aaacaacctt acttttgatc aatcagcaca gccccacact ttccattgcc
     3481 tatacttcct attctccatc cctgatccgc aaggccgaaa agatcacagg gctgtctctg
     3541 cacgaatatc aaattccttt ttcagcagaa actgtctatg gccgcgccat tcacaacaga
     3601 aaagcagtat tcgttgaatc agtcaaagag gccatgttgg agatcgtccc ctcccccatc
     3661 agccgctgga tagattcatt gatatccatg tttaaactgg ggcatggcat cctcgcgccc
     3721 ctgttggtgg acgatgaaac cctggggctg ttaaaaatca acggcacgtt cctaaaccaa
     3781 tctgatatac ccgccatgga agtttttgca gcccagatcg cgacagggtt atccaatatc
     3841 cgtctggtgc aaaaattgca ggatgaacta tacgcccgcc aaaaagcgga agaatccctg
     3901 caccgcagcc gtaaaacgct ccttgcattg gggcaggcgg cgcaatcgat ccagaaaacc
     3961 cacaccccgg aagacatcta tcgtgtggtt ggcgaacaga tcaaggcgct ggggcttgaa
     4021 gccaccattc tcacactcaa cagcaatcgt cagacactct gccatagcta caccaccctt
     4081 tccacagata tcattcaaaa tgccgaaaag atggcaaagg tatctgcaca aggatattgc
     4141 tggcaaattg acccaaacgg cacctataac cgcatcattt tttcaggcaa gtcagaattt
     4201 attcacaagt cggaagacct ctttgcagaa gcccttccaa aattcatgtg gcccatgacc
     4261 aaggctctta tgaaaatctt gaagattggg cagggaatca tcgcccccct gcgggtggac
     4321 gaagaagacc ttggtctgtt ggtcgtattt ggaaatgaag ccatcagtga agaggattgc
     4381 cccgccatcg attcctttgc gggccaggta gccatcagtt tgcggaacgc ccacctggcc
     4441 aaaaaagtgg aagctgaatt tctggagaaa aaacaagccg aagaagctgt gcgacgggcc
     4501 aaggaacatt tcgaagcctt gatcgaaaag gcccccgatg gcatcaccct gctgggcatg
     4561 gatgggaagg tcaaatacgc cagtccttct gcaaaaagac tgttcgatta cagcgaggag
     4621 gatataacga acctcacccc catggagttc attcatcctg aagacctcga cctcgttctt
     4681 gaaacaatga acaaactggt tcaaaaccct gcctacactc ccaccattca gtatcgttac
     4741 atgcgcaaag acggtgtcta tcattgggtg gagagcacct tcagcaacct gctgaccgac
     4801 cccaacgtgg aagcagtagt gatcaacttc cacgatatca cacaacgcaa gctcgttgaa
     4861 aaagccctgt tcgaaagcga aaagtattac cgcgcattga tcgaaaacgc ctccgatggc
     4921 atcctggttg taaatgaaga agggaagatc agttacgaaa gtccctctgt cacgcgcatg
     4981 ttaggctatg aaccgtacag cctgctcggg acaaatgcct tcgaactcat ccaccccgat
     5041 gacatggccg ccattataga agcattcaca caaggatcga gcactcccaa ccttgtccat
     5101 cgcggcgaat atcgcctgat tcgaaaaaat ggagaatggg gttactttga aattgtaagt
     5161 cactacctgc ttaccgaccc cgccatcaag gg
//
LOCUS       DAOQLC010000213         5130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7460_length_5130_cov_17.269163, whole genome shotgun sequence.
ACCESSION   DAOQLC010000213 DAOQLC010000000
VERSION     DAOQLC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_5130_cov_17.269163"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1520)
                     /locus_tag="PL000_20410"
     CDS             complement(165..1520)
                     /locus_tag="PL000_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91299.1"
                     /translation="MPTQNTIPTVVISNFAEAHIDFLGRSSDPEERDDLINGEDYHGD
                     RTILWADDPKLVIVNYPIAHAEFNVERLGYSRTSHLAPHEPSHYLCLDILREAHLLEG
                     ILQYAAPNGIVQLIPYATTKEFLQLVEVLRREHHLQVLTPESPDADHFWLRDYIDTKT
                     GFRTLASFWMTDASRFLPFGMICETLKHAAIVAHWFCSRGEACVVKTDTGESGIGTTV
                     ISPEDHLSVEGIFEKLHSQPFYSNELIVVEKYIPSANRISPSAEVKVPKLGEGEPQIT
                     YISNQLFLGFGDFCGIQIDRSIYSEPWCADLKRCTLELAHNLQNMGYVGHFDLDCIVS
                     DDNEVYLLEINARRTGGTHVHEFGKYTFGDDYVSKVSLISFEASNSGTITDAMELLEV
                     IKDFLYPMPGDEPLGMVVTITKPLFKNRFGCIVVAPTSERALQLQAQVDEHIQRYCQS
                     K"
     gene            complement(1529..2614)
                     /locus_tag="PL000_20415"
     CDS             complement(1529..2614)
                     /locus_tag="PL000_20415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91300.1"
                     /translation="MNETKKSRVFFISLRIKLLIGFTLLFTVVFAVAFYWFYQFSTDL
                     AMQRITDDLVNTIQGATKLIDGDEFQALYTEATPREDGYTDDLRYWAQAKLLWSVKQI
                     EPRAGIYTYIKGTEPNELIFVTSGGALNNPPTGAKFMEHWTTDNVGPNLSGLEELTLQ
                     NTPEGQKNNGCIYGTPGCKVEMYSDDFGSWVSAFAPIKNSKGEVVGAIGVDFKADYVR
                     EVQAAILKKVYIAFAITYISLFFLVFFISSAFTRPILTLTKAAEKIGEGDYKGGLVFL
                     SNVGETSKFPDEIETLDKVFQSMIDKVYTREQSLRKQVEELRIIIDDSKRKKQVDEIV
                     ESEFFQDLKTKAQKIRDNQARKKSDEA"
     gene            complement(2611..3333)
                     /locus_tag="PL000_20420"
     CDS             complement(2611..3333)
                     /locus_tag="PL000_20420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91301.1"
                     /translation="MFEIPELGFEIQEIVNGNTRYLDELIELYLQLFPGYGRYVAVMR
                     RRANRPNDVVPPFLEHQWLALVNGRPAAMTVFKYNYKRNCGLGLDLGVHPDYRNFSYS
                     GYTSLARLMIELRHKQLLMDAKLLGKPLPLGSLVEVESPKVVARFKEYGMLLLPVKYY
                     EPPAPEEALEIISRHELEKTGFNPMHLGIYPIDRDAYNPKDVDQISMCVKAYLMDHYG
                     LSETHWAVQESLQSISIEESAK"
     gene            complement(3333..4679)
                     /locus_tag="PL000_20425"
     CDS             complement(3333..4679)
                     /locus_tag="PL000_20425"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNC91302.1"
                     /translation="MNERITNWFGTQHVKLVLALWLLLAINMMVMELSDVVATAGFVS
                     NIGSDSLPWLWLVMTLLTLVGASGYSVLVDRYSRLNLITWLLGVMGVFYLFLMFLFRA
                     QIPDTITFPLLSIITDQQYYILPLAFWALANDVFSVTEGKRVFPLIASGAVVGSLAGN
                     SLAGLSAKLLEQLSAGTEAIFVLAALLLFVSVVVLRIAFFRHHVRARQSKDEDTSLMK
                     SMQVGVEYFTKVAMLKMVAIIMFLSGMALALLEFYFLRSIETTTSGNSLEFQQFLGYF
                     KTAQTIGLLTFQWLITRRLLEKVALKNSFMVLPVFLLSASGIALGLVGVWGVAIGRFF
                     ARMVQRGWDEPTRKSLQGLIPDERRGRVALFMDSVFYNLATIVSSMWMALLLWLMHSA
                     LITELTFQYMALGTALFAALGAMLASFNFNRVYEKSLLDWRFARSKRKSVLDNIEF"
BASE COUNT         1524 a         1266 c         1064 g         1276 t
ORIGIN      
        1 caccaagttc acaatggacg gttcgtcgtc aacgataagt atctttgtca tgatgctatt
       61 ctctcgcggt catttttatt catcataaag gcattgtaaa gatttggtaa agaaaaagcc
      121 acagtgaaca aggtctttca ctgtggcttt ttacaaaaat tttcctattt cgattggcaa
      181 taccgctgaa tatgctcatc tacttgagcc tgaagctgta gcgcgcgctc agatgtaggc
      241 gcaaccacga tacacccaaa tcgatttttg aataatggtt tggtaatggt gactaccatt
      301 cccagcggct cgtctccggg catcgggtag agaaaatcct tgattacctc gagcaattcc
      361 atggcatctg taatcgtacc agagttggaa gcctcaaaac tgatgagaga gaccttactg
      421 acataatcat caccaaaagt atattttcca aattcatgca catgtgtgcc cccagtgcgg
      481 cgggcattga tctcgagcaa atacacttca ttgtcgtcgc ttacgatgca atccaggtca
      541 aaatgaccga catatcccat attttgcagg ttatgtgcca attcaagggt gcaacgtttc
      601 aagtctgcgc accagggttc gctataaatg ctgcgatcaa tctgaatacc gcaaaaatcc
      661 ccaaatccta aaaacaactg attggatata taagtaattt gaggttcacc ctcccctagt
      721 ttgggcacct ttacttcagc cgatggagat attcgatttg ccgaggggat atatttctca
      781 acaacgatca attcgttgga atagaaaggt tgggaatgca gcttttcaaa aataccctca
      841 acagataaat ggtcttcagg agaaatgacc gtcgtaccga taccgctttc cccagtatcg
      901 gtctttacca cgcaagcttc tccacggctg caaaaccaat gcgcgacaat tgcggcgtgc
      961 ttcaaagttt cgcaaatcat gccaaatggc aggaatctac tggcgtccgt catccaaaaa
     1021 gaggccagag tacgaaagcc ggttttggtg tcgatataat cacgcagcca aaaatgatca
     1081 gcatcagggc tttcaggggt taatacctgc aagtggtgtt ccctgcgcaa aacttccacc
     1141 aactgaagaa attccttcgt ggtggcatat gggataagtt gaacaattcc atttggggcc
     1201 gcatactgca ggataccttc aagaagatgt gcttcacgta aaatatcaag gcacagataa
     1261 tgcgaaggtt catgaggagc cagatggctc gtacgtgaat agcccaaccg ttctacgttg
     1321 aactctgcat gggcgatcgg ataattgaca attaccaact ttgggtcatc agcccaaaga
     1381 atggtgcgat cgccatggta atcctcacca ttgatcaggt cgtccctctc ttcagggtcg
     1441 ctgctgcgtc cgagaaaatc aatgtgcgcc tcggcaaaat tactgatcac tactgtcgga
     1501 atggtattct gggttggcat ggaacacact aagcttcgtc gctctttttc ctcgcttgat
     1561 tatcacgaat tttctgggcc ttcgtcttga ggtcctggaa gaattcggat tctacaattt
     1621 catccacttg tttctttctc ttactgtcat caatgatgat cctcaattct tcgacctgtt
     1681 tacgcaggct ttgttcacgg gtatagacct tgtcaatcat cgactggaac actttgtcaa
     1741 gggtctcaat ctcatcgggg aatttggaag tttctcccac attggaaagg aaaaccaaac
     1801 ctcccttgta atcgccttcg ccgatcttct ctgccgcctt ggtaagtgta agaattggac
     1861 gggtaaatgc gctggaaata aaaaagacaa ggaagaacaa ggaaatatag gtgatggcaa
     1921 aggcgatata taccttcttc aagatcgctg cctgcacttc acggacataa tcagctttga
     1981 aatccactcc aatggcacct actacttcac ccttggagtt tttaatcgga gcaaacgccg
     2041 atacccagct tccaaaatcg tctgaataca tttccacctt gcatccaggt gtgccataga
     2101 tacagccgtt gttcttttgg ccttctggcg tattttggag ggtgagttct tccaagccag
     2161 aaagattggg accaacatta tcagtggtcc aatgctccat gaatttggca cctgttggag
     2221 ggttattcaa tgcaccgccg cttgtcacga agatcagttc gttgggttcg gttcctttga
     2281 tatacgtgta gatcccagca cggggctcaa tctgcttgac ggaccacaac agtttagctt
     2341 gcgcccagta gcgaaggtca tcagtatatc catcctcacg aggggtcgct tcagtataaa
     2401 gagcttgaaa ctcgtcacca tcaatcaact tagtggcacc ttgaatcgta ttcacaagat
     2461 catctgtgat acgctgcata gccaggtctg tggaaaattg ataaaaccaa taaaaggcaa
     2521 cagcaaacac aacagtaaac aacaaggtga aacctatgag cagcttaatc cgtaagctga
     2581 taaaaaacac cctgcttttc ttagtttcgt tcattttgca ctctcctcta tcgaaattga
     2641 ttgaagggat tcctgcacag cccaatgcgt ttcggataat ccataatgat ccattagata
     2701 agctttgaca cacattgaaa tttgatcaac atctttcgga ttgtaagcgt cccggtcaat
     2761 aggataaatt cccaagtgca tcggattgaa acctgtcttt tcaagctcgt gacggctaat
     2821 aatctcaagg gcttcttctg gagcaggcgg ttcataatac ttgactggca acaacaacat
     2881 gccatactcc ttaaatcgag ccacaacctt cggcgactca acctccacca gtgaccccaa
     2941 aggcaggggc tttccgagca gttttgcatc cattaaaagc tgcttgtgcc gcaattcaat
     3001 cataagcctg gcaagactag tataacctga ataggaaaag tttctataat caggatgcac
     3061 ccccaagtcc aggccgaggc cgcaattcct cttatagttg tatttaaaca cagtcatcgc
     3121 ggcagggcgt ccattgacca acgccagcca ctgatgctct aaaaatggcg gcacaacatc
     3181 attcgggcgg tttgcccgtc gtcgcattac ggcaacgtat cgcccgtacc cgggaaacag
     3241 ttgcagatac aactcgatca gttcatcaag gtaacgagta ttgccattga caatttcctg
     3301 gatctcaaat ccaagctctg gaatctcgaa catcaaaact ctatattatc caaaacgctt
     3361 tttctcttgg agcgtgcaaa acgccaatcc aacaggcttt tttcatacac acggttgaaa
     3421 ttgaaactcg ccaacatggc acccaaagcc gcgaacaggg ctgtgccaag ggccatatac
     3481 tgaaacgtta attccgtgat caacgcagaa tgcatcagcc atagtaagag tgccatccac
     3541 atgctgctaa cgatggtggc aaggttatag aaaacagaat ccatgaaaag ggccacacga
     3601 ccgcggcgct catctgggat caacccctgt aacgacttgc gggtaggttc atcccaacca
     3661 cgttggacca tgcgcgcaaa gaaacgtcca atcgcaacac cccacacccc caccaagcca
     3721 agagcaattc cgcttgcact gagaagaaaa acaggtaaaa ccatgaaaga gtttttcaac
     3781 gcaacttttt ccagcaaacg tcgggtaatc agccattgaa aggttaacaa cccgatggtt
     3841 tgagcagttt tgaagtatcc aagaaactgc tggaattcaa gggaattgcc gctcgtagtt
     3901 gtttcaatgg aacggagaaa ataaaactca agcagcgcca aagccatacc gctcaagaac
     3961 atgataatcg ccaccatttt aagcatggca acttttgtaa aatattcaac acccacctgc
     4021 atacttttca tcaggctggt gtcttcatct ttggactggc gcgcacggac atggtgacgg
     4081 aaaaacgcaa ttcgcaagac aacaaccgaa acaaacaaca aaagtgcagc caggacaaag
     4141 atcgcttcag tcccagctga aagctgctcc aacaatttag ctgacaaacc tgccagagag
     4201 tttcctgcca aactgccaac cactgcgcca gatgcaatca gaggaaagac tctcttccct
     4261 tcagtaaccg agaaaacatc attggcgagc gcccaaaacg caagcggcaa aatatagtat
     4321 tgctggtcag taattatgga aagtaaagga aaggtgatcg tatcgggtat ctgggcgcgg
     4381 aacaggaaca tcaaaaacag atagaacacc cccatcactc caagcaacca ggtgatcaaa
     4441 ttcaaccttg aatatcgatc cacgagaacc gaatatccgc tggcacccac tagcgtcaaa
     4501 agagtcatca caagccacag ccacggcaaa gaatccgacc caatgttact gacgaaacct
     4561 gccgttgcaa caacgtcaga taattccatc accatcatgt taatggcaag caaaagccac
     4621 aaagccagaa ccagtttaac atgctgcgtg ccaaaccagt tggttattct ctcattcaca
     4681 aaaccctcgt cggttaacaa ttttttggat ttgaagcaag ctccaatctc tttttttcaa
     4741 gattcaatga atggatttct ggcgccgtag acaaagcaac ggggacgccc ccgatcttcc
     4801 aaattccatt gaagaacgaa ctctctacaa cttgaaaatg gagcaaagct ctaaaatatt
     4861 ctttcctttt ggggaaaact aacgcgagta tacccgatta tgagaaaggc tccaattctg
     4921 caaaaatgcc tgaatttagt gtaataaatc aggaaaagcc atattacgat ccgaaccttg
     4981 gagtttcttt tttagaccca tacctcttac gctgggtaat cattaagaca agcaaatgtt
     5041 atacaagtga agcctcgtga aagtgtataa taaccttatg accgacgaac ccatcaaatc
     5101 actcgctcga aacaaaattg ccatgctgat
//
LOCUS       DAOQLC010000214         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7572_length_5083_cov_25.661893, whole genome shotgun sequence.
ACCESSION   DAOQLC010000214 DAOQLC010000000
VERSION     DAOQLC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7572_length_5083_cov_25.661893"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1841
                     /locus_tag="PL000_20430"
     CDS             <1..1841
                     /locus_tag="PL000_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91303.1"
                     /translation="PASPFTDGVIYWRVRGVNVAAVAGAWSTSRSFTVDTIAPLAPTL
                     STPANAAAVTGTPAFTWASVTGASKYQFEYDNNSDFSSPTYTSIELTTTSHTPPTISL
                     GSYSWHVRAKDAGGNWSPWSTARTVSITPVAPTGTVILSGPTNSAIVLDPTPTFTWGT
                     VTGTATYQIQVDRNSTFSSPDVDTNVVPTTYTPVSNLPDGTYYWRVRKVDVYGGVAPW
                     STVWRFSVNQPIPLAIKMSLSALSNEFNTGTPTFAWDAVEFGAQYQIQVDDSTEFASP
                     SFDDTAETTSRTLSEAMTNGDYFWRVRAINEYGTPGPWSEAWPFRINIPADAPALLSP
                     ADASEQSTGSPAFSWKSVTAAEEYQFQLDDSASFDDPEIEQSLSVTSYTPSKVLSDGE
                     YYWRVRSVNDLGSAGEWSDARTIVVSIPPLAPQLLTPVDGETLDAATNPTFSWEAVSN
                     GDTYQIQVDDSEDFGSPAFDDIASTTTRELPEPLTDGTYYWRVRAVNIYGAEGAWSDA
                     QAFTVTSVPVAPTLITPETDSGSAQTTATPTFSWEAVVNGVTYQIQVDNNEDFSSPEV
                     EDVAASTTRELTALGNGLYYWRVRAINMNGIPGEWSLVGSFTVNVP"
     gene            complement(1912..2562)
                     /locus_tag="PL000_20435"
     CDS             complement(1912..2562)
                     /locus_tag="PL000_20435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91304.1"
                     /translation="MKSIGYKALCSIFALALATLACATPDVSSLLNPMPSDDFSSDSS
                     GWGTGTDSSSSVEYANGGLQMIVYTPYYVTWSTQSIESMQNVHVEVSVKNESTDPEAL
                     YGIVCNELGSTQSFYYVGISPDGYYAFIKSAVAQDDVFLKEGNSDLISAAGGSVRLGL
                     DCGNGSMTLYVNGQQVDTVSDSTYTQGSIGLFAATDDQESGVNVTFDDYVVTKLGE"
     gene            complement(2630..5035)
                     /locus_tag="PL000_20440"
     CDS             complement(2630..5035)
                     /locus_tag="PL000_20440"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNC91305.1"
                     /translation="MYNSSMPLLWISLLFLAGIIISSLLSFNLYIWIIAGGALILITS
                     LFVSSRVSSSSILKPRFEIRLSHLVYIFFFFAGGAWYQFRQPNIDVSQVAFYNDREYE
                     ILVTGRLVEPPDYRDSYTNLHLRVEAVDSGSGDLPAQGELLVRVPPNQKYEYSELIRV
                     RGKLKTPPEDDEFSYREYLARQYIYSYMPIAEVTRLPWKTENPLVSEIYKLKDSLLNN
                     TYRLYHDPEASLLAGILFGVDTGLTRELQDAFKNTGTAHIIAISGFNIAIIAGIFFFF
                     FKSFLGERFGAVAAVIGIFFYAFLVGGDSAVMRAAFMGSISLAARQLGRRNVGVNALA
                     VVAFVMAFFNPLVLWDIGFQLSFFATLGLILYAEPFSNFTSNLISKFSKGDTSTITRI
                     INEYFILTLAAQLTTIPIMAYHFKRISLISFIANPFILPVQPAVMIGGGLAVFISLII
                     YPVGQFLAWLAWPFATYTIRMVELFDKVPHASIYLGDSPAWIITSFYIALLTVTFGWS
                     AITEWFSTRKTLLRSLALTGIIMLLFSCTLVFWRSAATAGDGEFHITFLEVGSADAVL
                     IQTPEGRNVLVNGGASVSELSDELGRRLPFFSRKLDWLVIASTQESQIEALPRIVERY
                     PPDNVLWSGNMQASFAAQELDKYFAAHEIPITLAETGQRLELGGGAFIEIQASGPKGS
                     VLVIQYGNFRALLPIGIGEGSEDALNFENVLDSKVDVLLLADSGYAPSNPPELFELLR
                     PQLVVLSVSAGDPDGLPSQEVLEALDGFSLLRTDRNGWITITTDGETMRVDVERGETI
                     ATP"
BASE COUNT         1240 a         1291 c         1393 g         1159 t
ORIGIN      
        1 cccccgcctc tccgttcacg gatggcgtga tctactggcg tgtacgcggt gtgaacgtgg
       61 cggcagttgc cggtgcgtgg agcacttccc gctcctttac agtggatacc atcgcgcctc
      121 ttgcgccgac cttgagcact ccggccaatg cagcggcggt taccggcact cctgccttca
      181 catgggctag tgtcactggt gccagtaaat atcagttcga gtatgacaat aactcagact
      241 tctcaagtcc gacctatacc tcaatcgaac tgactaccac cagccatacc ccgcccacaa
      301 tttcccttgg ctcctactcc tggcatgtcc gcgcaaagga tgctggtgga aactggagtc
      361 catggagcac ggcgcgcacg gttagcatca ccccggtggc tccgactggg acggtcatcc
      421 tctccggccc gaccaatagc gcgattgtcc ttgacccgac tcccaccttc acatggggta
      481 cggttacagg tactgccacc tatcagattc aggtggatag gaactccacc ttcagcagcc
      541 cggatgttga tacaaatgtt gtacccacaa cctacacccc ggtctctaat cttcctgatg
      601 ggacatatta ctggcgtgta cgtaaggtgg atgtgtatgg cggtgtagct ccgtggagta
      661 cggtctggcg ctttagtgtt aaccagccga tcccgctggc gatcaagatg tcgctgtcgg
      721 cactctcgaa cgagttcaac accggcaccc ccacctttgc atgggatgcc gtggaatttg
      781 gcgcccaata tcagattcag gtggatgaca gcactgagtt cgccagtccg tcctttgacg
      841 atacagctga aaccaccagc cgcacgttgt ccgaagccat gacgaacggt gattacttct
      901 ggagagtccg tgccattaat gaatatggca cccccggtcc ctggagtgag gcttggccgt
      961 tccgcatcaa catcccggcg gatgcacctg ccttgctgtc gcctgcggat gcctccgagc
     1021 aatccactgg ctcccccgct ttctcctgga agagcgtcac tgctgctgag gaataccagt
     1081 tccagttgga tgatagcgcc agcttcgatg atcctgaaat cgaacagtct ttgtcggtca
     1141 ccagctacac tccgtccaag gtgctgagtg atggtgagta ctactggcgt gtccgttcgg
     1201 tgaatgatct tggctcggca ggcgagtgga gcgatgcgcg cactattgtg gttagcatcc
     1261 caccgctggc cccgcagctc ctcacccccg tggatggtga aactctcgac gcagcgacca
     1321 acccaacgtt cagttgggaa gcggtgagca acggcgatac ttaccagatc caggtggatg
     1381 attccgaaga tttcggcagt cccgctttcg atgacatcgc atctaccacc acccgtgaac
     1441 tgcccgagcc gctgacagat ggcacgtact actggcgtgt ccgcgccgtg aatatctacg
     1501 gtgctgaagg tgcatggagc gatgctcagg ccttcacagt cacctccgtg cctgttgcac
     1561 caaccctgat cacacctgaa actgattcgg gaagtgccca aactacggcc acacccacat
     1621 ttagctggga ggccgtggtc aatggtgtca cctaccaaat tcaggtggat aacaacgaag
     1681 acttcagcag ccccgaagtt gaagatgtgg ccgcttccac cacccgtgaa ttgaccgcac
     1741 ttgggaatgg tctgtactac tggcgtgtcc gtgccatcaa tatgaacggc atccctggtg
     1801 agtggagtct cgttgggagc tttacggtga acgttccgta gaaaatataa aagcaacaaa
     1861 aaatctccgt gccgaaaagg cacggagatt tttattttta ggctttgaag attactcgcc
     1921 gagcttggtt acaacataat catcaaatgt gacattcacg ccggattcct ggtcatcggt
     1981 ggcggcgaag aggccaatgg atccttgagt gtatgtggag tcggaaacag tatcaacctg
     2041 ctgaccattc acatacaggg tcatcgagcc gtttccacaa tccaaaccga ggcgtaccga
     2101 accacccgcg gcggagatca ggtctgaatt gccttccttg aggaaaacat catcctgagc
     2161 cactgcagat ttgatgaagg catagtagcc atcgggggaa atgcccacgt aataaaatga
     2221 ttgggttgag ccgagttcat tgcagacaat gccataaagg gcttcagggt cggtgctttc
     2281 gttctttaca ctgacctcca catggacgtt ttgcatcgac tcgatgcttt gggtcgacca
     2341 ggtgacgtag tagggggtgt acacgatcat ctgcaaacca ccattggcat attccacaga
     2401 gctggagctg tctgtgccgg tgccccaacc gctgctatcg cttgaaaaat catcgctggg
     2461 cattgggttg agaagcgagc tgacatctgg tgtggcgcag gccagtgtgg caagtgcaag
     2521 ggcaaaaatt gaacaaagtg ctttgtaacc gatagatttc atgcaattct cctttttatg
     2581 tgggcggtat tttagcatct cacccacacg ggcaaagggt actttgctcc tatggtgttg
     2641 caatggtttc gccgcgttcc acatccacac gcatggtttc gccgtctgtt gtgatggtga
     2701 tccaaccatt tcgatcggtt cgcagcagtg agaacccatc gagagcttcc agcacttcct
     2761 gcgagggcag gccgtcaggg tcgcctgctg aaacgctcaa caccaccaat tgcgggcgca
     2821 ggagttcgaa caattcaggc ggattggaag gtgcataccc ggaatcggcc agcagcaata
     2881 catccacttt tgaatcaagt acgttttcaa aattaagcgc atcttcgctg ccttccccga
     2941 taccgatggg gagaagtgcg cggaaattcc cgtattggat gacaagcacg cttccttttg
     3001 gacctgaggc ctgaatctcg atgaatgcac caccacccaa ctcaagcctt tgtcctgttt
     3061 ctgcaagcgt gatcgggatt tcatgtgcag caaaatattt gtccagttcc tgtgcggcga
     3121 aagaggcttg catgtttccg ctccacaaca cgttatcggg cgggtatctt tccacaatgc
     3181 gcggcagggc ttcgatctga ctttcctgtg tggaggcaat gacaagccaa tcgagtttac
     3241 gtgagaagaa tgggagccgc ctgcccaact catctgaaag ctcagaaaca ctcgcccctc
     3301 cgttgaccag tacattgcgg ccttcggggg tttggatcaa tactgcgtcg gctgagccaa
     3361 cttcgagaaa agtaatgtga aattcaccgt cacccgcggt ggctgcggag cgccaaaaga
     3421 caagggtgca ggagaacagc agcatgatga ttcctgtcag agcgagagat cggagcaggg
     3481 tttttcgggt gctgaaccac tctgtaatgg ccgaccagcc aaaggttaca gtaagaagcg
     3541 cgatatagaa ggatgtgatt atccaggcag gcgaatcccc aagatagatg gaggcatgcg
     3601 gtaccttgtc aaagagttcc accatgcgga tcgtgtatgt ggcgaacggc cacgcgagcc
     3661 aggcgaggaa ctggccgact ggataaatga tcaggctgat gaagaccgcc agtccgccgc
     3721 cgatcatcac tgcgggttgt actggcagaa tgaaagggtt ggcgatgaac gaaataaggg
     3781 agatgcgttt aaagtgatag gccatgatgg gaatggtggt taattgtgcg gcaagggtca
     3841 gtatgaagta ttcattgatg atgcgggtga tcgtgcttgt gtcacctttt gagaatttgg
     3901 aaatgaggtt gctggtgaag ttggaaaatg gttcggcgta gaggatgagg ccgagggtgg
     3961 cgaaaaatga aagctggaaa ccgatatccc acaatacaag cgggttgaaa aatgccatga
     4021 cgaatgcaac aactgccagt gcatttaccc caacattacg cctgccaagt tgacgggctg
     4081 ccagtgaaat gctgcccatg aaggcggcac gcatcaccgc agagtcaccg cctacaagaa
     4141 aggcgtaaaa aaatatgccg atgaccgcgg caactgcgcc aaatcgttca ccaagaaatg
     4201 atttgaagaa gaaaaagaaa atacctgcaa tgatggcaat attaaagcct gagatggcta
     4261 tgatgtgggc ggtgcctgtg ttcttgaagg catcctgtaa ttcgcgggtg agaccggtat
     4321 ccactccgaa aaggatgcct gccagcagag aggcttccgg gtcatgataa aggcggtagg
     4381 tgttgttgag cagggagtct ttgagtttgt agatctcaga aaccagcgga ttttccgtct
     4441 tccagggcag gcgcgttact tctgcaattg gcatgtacga gtaaatatat tggcgggcaa
     4501 gatactcacg gtaggagaat tcgtcgtctt cgggcggggt ctttaatttt ccgcgcacac
     4561 ggatcaattc actatattcg tatttctggt tggggggcac gcggactaaa agctcgcctt
     4621 gagcgggcag gtcgccgctg cctgaatcta cagcttctac tctgaggtgc agatttgtgt
     4681 acgaatcgcg gtagtcaggc ggttccacta atctgcctgt aacgagtatc tcgtattcgc
     4741 ggtcgttgta aaaagccact tgtgaaacat caatgttggg ctggcggaat tgataccatg
     4801 ccccgcctgc aaagaagaaa aagatataaa ccagatggct gagcctgatc tcaaagcgtg
     4861 gttttaagat ggagcttgag gagacgcgcg aagatacgaa taggctggtg ataaggatga
     4921 gggctccccc ggcaatgatc caaatataca agttgaaaga aagcaggctc gaaatgatga
     4981 tacctgcaag aaaaagaagc gaaatccaca gcaaaggcat gctggaattg tacattagat
     5041 ttttggatta tgagtttggg aagtgagcac aacccaacag gat
//
LOCUS       DAOQLC010000215         5081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7576_length_5081_cov_26.043971, whole genome shotgun sequence.
ACCESSION   DAOQLC010000215 DAOQLC010000000
VERSION     DAOQLC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7576_length_5081_cov_26.043971"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..3128)
                     /locus_tag="PL000_20445"
     CDS             complement(63..3128)
                     /locus_tag="PL000_20445"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC91306.1"
                     /translation="MDRILETFKIKPGEERMSALLMGLMLITAAGGAIGGNATEALFF
                     ARFGVEFLPTMYVILGVFTFLTSMAITIVMGRFSKQRLFTTLPFFIGLILIVEWIVLR
                     FDIRWFFAAMWLGMNVINSLQGLAIWGLAGTACDTRQAKRLFPLFSAGGILGAVLGGL
                     LTQSLARWLHSENLLLVWAGTLFIAFLLGRALIGRSVGPNITSARIKQPGIFVEMQRG
                     YNFVRRSPIMQWVSYSAILFSVCYFSLALPFSRGATAQFTDADRLAGFLGLFQSITTI
                     VALIVSLFFANRLFARFGIMPMLLLFPIIYFVGFGVLSFSAPFAILVSVRFMQMMWMQ
                     GIAGTAWQALFNVVPSEQRDQVRTFIGGVPEQAGTFIAGLILVVGEQTLAPGQLYFIG
                     LIAAAFLIYMLWRASRDYSIALVEALRAGQPQMFVSEEEPFGGFSTDATAVAAALKGM
                     SDPDRLTRLVSIEILGHLSTPKVIAGMVSALTDEDAMIRSSALRGLTHARASSALLEI
                     AASLSDPEPEVRLQAVDSLRQLAQHPRGVSAHIEPLLTDQDPLVRTRSAQTLLSFGEH
                     PKAKSILHEMAAGTDITSRTHALNALAECGDESAFELGLSALDDAAPIIRKTAPRLLV
                     SIDPQRALEPILHHLNDDDSHVRKALAEAIGNIGLPALDSLLAALTNPSYEDGALTAL
                     ELLPAQKASQQIRVHAMNTARSALHYHQLALGLAQKFGRTISGDGRYQLLIESLHDKA
                     HRSGHNSLRAVGLLKDRQTIGIAIENLRSRESAQRANALEVLESIGEVEIIRPLLALW
                     ESGESSSASLPDQWLMELLHDPYSWLRACAVLAAAESDDEKIVLTLGSLSRSDPDATV
                     RDIADKVISGGHSMDTLATLPLMERILFLRRVPIFADLSPADLKQVAAITTEAVFADG
                     DILAHQGEFGDEMFIVVSGEVNVLIASDDQKESRYIAKRSTGDYVGEMSIISREPRIA
                     TLVASGQVRALCVGRKQFEGILRERPEIGMALMRTLCQRLKEASLQQTN"
     gene            3260..>5081
                     /locus_tag="PL000_20450"
     CDS             3260..>5081
                     /locus_tag="PL000_20450"
                     /inference="COORDINATES: protein motif:HMM:NF013124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC91307.1"
                     /translation="MRELPFGTVTFLFTDIEGSTKLWEQHPEAMKTALAKHDSILKDA
                     IESNRGHVIKTTGDGFHAVFATAMDGINASVTAQRELHSSFFLHPSSLSLKVRMGIHT
                     GEAELRNNDYYGGTLNRAARLMSVANGGQVLLSNTTADLVREQLPAEITLRDLSEHRL
                     KDLTRPERIFQIVHPELPSEFPPIKTLDAFPNNLPAQLTSFIGREKEINEIINALNTS
                     RLVTLTGSGGTGKTRLSVEVGSQLLTNFQHGVWLVELAPLSNEAQIIPALAQAFGLQE
                     LPFNPLAKLVDDYLRDKKLLLILDNCEHLIDACARLADHLLHLSAGVKILASSREALG
                     IAGEVAYRTPSLADSESTSLFLDRARAANSKFSLTESNASAIAQICHRLDGIPLAIEL
                     AAARTKILSAEQIATRLDDMFRLLVGGSRTALPRQQTLRALIDWSYDLLSDEEKRLLQ
                     FASVFVGGWTLDALEFVANEPNTLELLEQLVNKSLVVTEEREGEMRYFLLETIRQYAR
                     EKLFEAKQASAARDRHFIHYKNSTSGLWDVSRFNNETELQRLKAMQGEMENLRAAFEW
                     GLQNHVQDALDLAANMATSITMIGGPMEGTAMLKMAMEKFR"
BASE COUNT         1260 a         1291 c         1399 g         1131 t
ORIGIN      
        1 aaggaggctg tcgccataaa agtgacagcc tcctttttat ttaatcgatg ccagaaatgg
       61 tattagtttg tttgttgcag gcttgcttcc tttagccttt gacacagggt gcgcattaat
      121 gccattccga tctcggggcg ttcgcgcagg atgccttcaa attgttttcg acccacgcac
      181 aaggcccgca cctgccccga tgcgaccagg gtggcaatac gcggttcacg gctgataatg
      241 gacatttccc caacataatc gccagttgac ctttttgcga tgtagcggga ttccttttga
      301 tcatccgagg cgatcagtac attcacttcc ccggaaacga caatgaacat ttcatcgccg
      361 aattctccct gatgtgcaag gatatctcca tcggcaaaaa cggcctcggt tgtgatcgcc
      421 gctacttgct ttaagtcagc gggggaaaga tctgcaaaga tgggtacgcg ccgcaggaaa
      481 agaatacgtt ccatgagcgg aagggtggca agtgtatcca ttgaatgacc tcctgaaatt
      541 actttatcgg caatatcgcg aacggttgca tcggggtctg atcttgaaag agatccaaga
      601 gtgagaacaa tcttctcatc atcagattcg gcggcggcca aaacagcgca ggcacgcagc
      661 catgagtagg gatcatgaag cagctccatc agccattgat cgggcagaga tgcggaagac
      721 gactctccag attcccataa agccagcagc gggcggataa tttccacctc gccaattgat
      781 tcaaggactt cgagcgcatt cgccctttga gcggattcac ggctgcgcag gttttcaatg
      841 gcaatgccga tggtctgacg gtctttcagc aaaccaaccg cccgcaggga attgtgtccg
      901 ctgcggtggg ctttgtcgtg caatgactcg atcagcaatt gataacggcc gtcacctgaa
      961 atggtccgcc cgaacttttg agcgagcccc aatgccagtt gatgatagtg cagtgcggat
     1021 ctggctgtat tcattgcatg gacgcggatt tgctggcttg ctttctgggc gggcaggagt
     1081 tcaagtgctg tcaaggcgcc gtcttcataa cttgggttgg tcaaggctgc tagcaatgaa
     1141 tccagtgcgg gaaggccgat gttcccgatc gcttctgcaa gcgccttgcg gacgtggctg
     1201 tcgtcatcgt tgaggtgatg aaggatgggt tccagtgcgc gctgcgggtc gatgctcacc
     1261 aacaatcgcg gcgcggtctt tcgtatgatg ggggcggcat cgtcgagggc tgatagtccc
     1321 aattcaaagg cagattcatc cccgcattca gccaatgcat tcaatgcatg tgtacgggag
     1381 gtgatgtctg tgcctgccgc catttcatgc aggatgcttt ttgctttggg gtgttcaccg
     1441 aagctcaaca aagtttgagc agagcgggtg cggacgagcg ggtcttgatc tgtcaacaag
     1501 ggttcgatgt gggcggatac cccgcgtgga tgctgagcca actgccgaag ggagtctact
     1561 gcttgaaggc gcacctcagg ctcagggtcg gaaagactcg cggcaatctc cagaagggcg
     1621 gaggatgccc gggcgtgagt caggccgcgc agggcagaag agcggatcat ggcgtcttca
     1681 tctgtaagtg ccgagaccat gccagcgatc actttgggag tggataagtg cccaaggatc
     1741 tcaatggaca ccaggcgggt gaggcgatcg ggatcgctca ttcctttaag agccgcagca
     1801 actgctgttg catccgtgct gaaaccaccg aagggttcct cttcgctgac gaacatttga
     1861 ggttgacctg cgcgaagcgc ctcgaccaat gcaatgctgt aatcgcgact cgcacgccag
     1921 agcatgtaga tcaaaaacgc ggcggcgatg agcccgataa aatataactg ccccggggcc
     1981 agggtttgct cgccgaccac aagaattaac cccgcaataa aagtgcccgc ctgttcaggc
     2041 acaccgccaa tgaatgttcg gacctgatcg cgttgttcag acggcaccac attgaaaagt
     2101 gcctgccagg ctgtgcctgc aatcccctgc atccacatca tttgcataaa acgcacagaa
     2161 actaaaattg caaaaggtgc ggagaatgaa agcacaccaa acccgacgaa gtaaatgatc
     2221 gggaagagca aaagcatcgg catgatgcca aaacgggcga aaagacgatt ggcaaaaaac
     2281 aatgaaacga ttaaagcaac aatggtggtg atgctttgaa ataaccccag aaaccccgca
     2341 aggcggtcgg catcggtgaa ttgagcggtg gcaccgcgtg aaaatggaag agcgagtgag
     2401 aaataacaga ccgaaaataa aatggcactg tatgaaaccc attgcatgat cggggatctg
     2461 cgcacgaagt tgtatccgcg ctgcatttcg acgaagatac cgggttgttt tatccgtgct
     2521 gaggtgatat tgggaccgac cgacctgccg atcaaagcac ggccaagaag gaaggcgatg
     2581 aataaagtgc cagcccaaac cagcaatagg ttttctgaat gcagccagcg cgccaatgat
     2641 tgagtcaaca agccgccaag caccgcgcct aagattccgc ctgcactaaa gagagggaac
     2701 aaccgtttgg cttgccgtgt atcacaggct gtacccgcca acccccagat ggccagccct
     2761 tgcagactgt tgatgacgtt catgcccagc cacatggcgg caaagaacca gcgaatatcg
     2821 aaacggagga cgatccactc gacgatcaat atcagcccga tgaaaaatgg aagggtggtg
     2881 aacagccgct gttttgaaaa acgccccatg acgatggtaa ttgccatgga ggtgaggaag
     2941 gtgaacacgc ccagaatgac atacatggtg ggcaggaatt ccacaccgaa gcgtgcaaag
     3001 aacaaggctt cggtggcgtt cccaccgatc gctccgcctg ctgcagtgat cagcatcagc
     3061 cccatcaaca gcgcagacat gcgctcttcg ccgggtttga tcttgaacgt ttcgagaatt
     3121 cgatccattt ggcgcctatt ggattcgacg tacaggaata tgcattaagg ttattgtact
     3181 taactatatt ggattagaat agtccaaata tccacgtgga tgtggttttt gtcaaagggg
     3241 ctgcttgtga ggatggaaca tgcgcgaact tccgtttggc acagtgacct ttctttttac
     3301 cgatatcgaa ggctcaacca aactttggga gcagcatccc gaagcgatga aaaccgcgct
     3361 tgcaaaacac gattcgatct tgaaagatgc cattgaatcg aatcgtggac atgtcatcaa
     3421 aaccacgggg gatgggtttc atgcagtgtt tgcaactgcc atggatggca tcaacgcatc
     3481 cgttacagcg cagcgcgaac ttcattcctc cttctttctt catccttcat cattgtcatt
     3541 gaaagtccgc atggggattc atacaggcga agctgaattg cgtaacaatg attattatgg
     3601 cgggacgttg aatcgtgcgg cgcgattaat gtctgttgca aatggcgggc aggttctgct
     3661 ctcaaatact acagcagatc ttgtccgcga gcaactaccc gcggaaataa ccttgcgaga
     3721 tctgagtgag caccgcctca aagatctgac ccgtcctgag cgcatttttc aaatcgttca
     3781 tcccgaactt cccagtgaat tcccgcccat caaaacgttg gatgcgtttc cgaataatct
     3841 ccccgctcaa ctcacttcgt ttatcgggcg cgaaaaagag atcaacgaaa tcatcaacgc
     3901 gcttaataca tcacgtctgg tcactctcac tggctcgggc ggcacgggca agacgcggct
     3961 ctctgtcgaa gtcggctcgc aactgctgac caattttcag catggtgtgt ggctcgtgga
     4021 actagctccg ctcagcaatg aggcgcagat cattcccgcg ttggcgcagg cctttggttt
     4081 gcaggagttg ccgttcaatc cgctggcgaa gctggtggat gattacctgc gcgataaaaa
     4141 gctcctgctg attctcgata actgcgagca tctcattgac gcctgcgcac ggctggccga
     4201 tcatttgctg catctcagtg cgggagtcaa aatccttgcc agcagtcgcg aagcgttggg
     4261 tattgcaggc gaagtggcgt atcgaacacc atcgcttgcg gattccgaat cgaccagcct
     4321 tttcttggac cgtgcccgtg ccgccaactc aaaattttcc ctgaccgaat caaacgcttc
     4381 tgccatcgcc caaatctgcc accgtctcga tggcattccc ctggcgattg agctcgccgc
     4441 cgcgcggacg aagattctct ctgcagaaca aatcgcgaca cggctggatg atatgttccg
     4501 cttgcttgtg ggtggaagcc gcaccgcgtt gccacgtcaa cagactttgc gtgcattgat
     4561 cgactggagc tacgatttac tctccgatga agagaaacga cttttacaat ttgcgtcggt
     4621 gtttgtcggc ggatggacgc tggatgcgct cgaattcgtc gcgaatgaac cgaatacgct
     4681 ggagttattg gagcagctcg tcaacaagtc gttggtcgtg acggaagagc gcgaaggcga
     4741 gatgcgttat ttcctattgg agacgatccg gcaatatgcg cgggaaaagc tgttcgaggc
     4801 gaagcaggca tcggcggcgc gtgatcggca cttcatccac tacaaaaata gcacgagcgg
     4861 tttatgggat gtctcgcgct tcaacaacga aacggaattg caaaggttga aagccatgca
     4921 gggcgaaatg gaaaatttgc gcgcggcgtt tgaatggggt ttgcaaaatc acgtgcaaga
     4981 tgctttggat ctggcggcga atatggcgac gagcattacc atgataggcg gtccgatgga
     5041 agggaccgcc atgctcaaaa tggcaatgga gaagtttcgc g
//
LOCUS       DAOQLC010000216         4954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7893_length_4954_cov_23.584813, whole genome shotgun sequence.
ACCESSION   DAOQLC010000216 DAOQLC010000000
VERSION     DAOQLC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7893_length_4954_cov_23.584813"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="PL000_20455"
     CDS             complement(<1..1352)
                     /locus_tag="PL000_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC91308.1"
                     /translation="MTTNELETFLAQYPTYKNTEAIDKLRKSDYARLDAGEHVYLDYT
                     GGGIYAESQIQKHHKLLNENVYGNPHSSNPTSQAATHLVEGTREYILKFFSADPDEYL
                     AIFTPNASGALKLVGESYPFPNGRYLLTFDNHNSVNGIREFAHARGAEVTYIPVMLPE
                     MRIDASQLDLELARPSKNGYNLFAYPAQSNFSSVKHPLEWIEKAHAHGWDVLLDAAAY
                     VPTNKLDLSKVKPDFVPISFYKIFGYPTGLGALIARKSALAKLHRPWFAGGTITVASV
                     QGDKYYLADGAPAFEDGTLDYLNIPAIEIGLKHIESIGYDSISERVRTLTGWLLQNLT
                     AMKHSTGEPLVRLFGPATSEGRGGAVTVNFYDKNGKAFDHRFIESEANKVNISLRTGC
                     FCNPGAGEVALGISRVELDVCFTSPDHKDKMSIDDFRLCIDGKSSGAVRISVGMVTNF
                     "
     gene            complement(1429..2187)
                     /locus_tag="PL000_20460"
     CDS             complement(1429..2187)
                     /locus_tag="PL000_20460"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNC91309.1"
                     /translation="MKIVRYQTNGEAVKYGWMLDDKVGEIQGDIFGEYRRREAKIPVG
                     ELKLAAPCVPSKILCVGRNYVEHAKELGNEVPKVPLIFLKPPSSIISNGEHIVLPPQS
                     SQVEHEAELVVVIGKRGRNITIENAKEYVFGYTIGNDITARDLQKTDGQWTRAKGFDT
                     FCSFGPWIDTEFDASDAVVTCRVNGQMRQMASTRDMVFNINTLIAFISSVMTLEAGDI
                     IFTGTPSGVGELKNGDMVDVEIEGLGKLSNPVKS"
     gene            complement(2331..3017)
                     /locus_tag="PL000_20465"
     CDS             complement(2331..3017)
                     /locus_tag="PL000_20465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNC91310.1"
                     /translation="MDIEVVHRPSYSLAIAKLTPNERIRAEAGAMVSMSEGVQIETKA
                     EGGFLKSLGRAVLGGESFFQNFFVAPPQGGEVTLAPELPGDITVIEMSGGKLMIQSGS
                     YMASESTVELTAKVSVKALMSAEGVSMLEATGTGTLLVSSYGAIYEKNLNAGEKYTVD
                     TSHLVAFDGNMGVTPKPIGGLKSTLFSGEGFVIELSGPGKIYIQTRSPQALINWIIPQ
                     LPKPPSSSNH"
     gene            complement(3088..3567)
                     /gene="folK"
                     /locus_tag="PL000_20470"
     CDS             complement(3088..3567)
                     /gene="folK"
                     /locus_tag="PL000_20470"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC91311.1"
                     /translation="MNHIVYLALGSNMGNRMMNLKTAISNLTPQMTVKQKSSVYETPP
                     WGFTEQAAFLNQVLKVETYLKPEALIAHLKRLESALGRVPNFTNGPRLIDIDILFFDD
                     MVIYTPPLVVPHPRLHERAFVLLPLAEIAPDLIHPVLHKSVSELLDDAERIEIKIVK"
     gene            complement(3595..4434)
                     /locus_tag="PL000_20475"
     CDS             complement(3595..4434)
                     /locus_tag="PL000_20475"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HNC91312.1"
                     /translation="MSSFKQRTLDFLEIEWATYIQRFERWPADEGLKRVNAQGYEQFR
                     DMLAHVLAWWEEGMGIILAIAEDREYARKKYDFDAFNAEAVAKYKGWNEAQFLAHFEK
                     TRQKTVADLRSMNKAAWENRRVQAWVNGIFINHAREHLVASSRFLILDTLEHGWGTYI
                     EDFEKVEDKAAFLSKQGVENFRELLGHAIGWWEEGERIIKGILSDPNFTWQDRDTDAF
                     NRELIVKYNQLSDEEVQKQFESKRQDMVRFVKGLPDSAFTNKDIEGWLAADIAEHYDE
                     HRP"
     gene            complement(4481..>4954)
                     /locus_tag="PL000_20480"
     CDS             complement(4481..>4954)
                     /locus_tag="PL000_20480"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNC91313.1"
                     /translation="METPPVSIALEQLGVPHTIFRHDDARPVHSLEQAASDRGQRASQ
                     VVRSILFRVAEDEFIMALVAGPAQISWKTLRKHLGKSRVSMASEDEVLAVTGYRIGAV
                     GPFGLLNQVKVLIDPSVMLEEEISIGSGMRNTAVILKSADLHHALKNSEIVALTE"
BASE COUNT         1089 a         1304 c         1317 g         1244 t
ORIGIN      
        1 ttgaagtttg tgaccatgcc cacagagatg cgcacagcgc ccgaggattt tccgtcaatg
       61 cacaggcgga agtcgtcaat gctcattttg tctttgtgat cgggagaggt gaagcacaca
      121 tccaattcca cacgggagat gccgagcgcc acttcacccg caccgggatt gcagaagcag
      181 cctgtccgca gtgagatatt gaccttgttg gcttcacttt cgataaaacg atgatcgaag
      241 gctttgccgt tcttgtcgta aaaattgacc gtgaccgcgc cgcctcttcc ttcactggtg
      301 gctggtccaa ataaacggac aagcggctcc ccggtgctgt gtttcatggc ggtcaggttt
      361 tgcagcagcc agcctgtcag cgtcctgacc cgttcactga tcgaatcgta tccgatggat
      421 tcgatatgct tgagtccgat ctcgatggcg gggatgttga gataatcaag cgtgccatct
      481 tcaaaggcgg gagcgccgtc ggcgaggtaa tatttgtcgc cttgcaccga ggcaaccgtg
      541 atggttccgc ctgcaaacca ggggcggtgc aattttgcca gcgcagattt gcgtgcgatc
      601 aatgcgccca gccctgtggg gtagccgaag atcttgtaga aagagatcgg cacaaaatca
      661 ggcttgacct tgctcagatc gagtttgttg gtgggcacgt aggcggcggc atccagcagc
      721 acatcccaac cgtgggcatg ggctttctcg atccattcca gcggatgttt gaccgaggag
      781 aagttggatt gcgcgggata tgcaaaaagg ttgtatccat tcttcgaagg ccgtgccagt
      841 tccaggtcca gttgtgaagc gtcgattctc atttcaggca gcatgactgg aatgtaggtc
      901 acttccgcac cacgggcgtg tgcaaattcc cgaatcccat tcaccgaatt gtgattatcg
      961 aaagtcagca agtaacgccc gttcgggaag ggataacttt cgccgacgag tttgagtgcg
     1021 ccgctggcat tgggggtgaa aatggcaaga tattcgtctg ggtcggcgct gaagaatttg
     1081 aggatgtact cgcgcgtgcc ttcaacaagg tgagtggccg cctgtgaggt ggggttggat
     1141 gaatgcgggt tgccatagac attttcgttc aagagtttgt gatgcttttg aatctgtgac
     1201 tcggcgtaga tgccgccgcc cgtgtaatcg agataaacat gctcgcctgc atcgaggcgg
     1261 gcataatctg atttgcgaag tttatctatg gcttcggtgt ttttgtaggt ggggtactga
     1321 gcaagaaaag tttcgagttc gtttgtggtc ataatgggtt cctgttttgt agggcgggtt
     1381 tgcagcccgc catgttttgt ggtcaaaaag tcaggcagga agcctgacct acgatttaac
     1441 agggttgctt agcttgccga gaccttcgat ctccacatcc accatgtcgc cattctttaa
     1501 ttcgcccaca cccgatggcg tgcctgtgaa aataatatcg cctgcttcga gtgtcatcac
     1561 tgaagaaatg aaggcgatca aggtgttgat gttgaaaacc atatcgcggg tggatgccat
     1621 ctggcgcatc tggccgttca cacggcaggt caccacagca tcggaggcgt cgaactcggt
     1681 gtcgatccac gggccgaaag aacagaaggt gtcgaaccct ttggcccggg tccattgacc
     1741 gtctgttttt tgcaggtcgc gcgcggtgat gtcgttgccg atggtgtagc cgaagacgta
     1801 ttccttcgcg ttttcgatgg tgatgtttcg tccgcgtttg ccgatgacga cgaccaattc
     1861 cgcttcgtgc tccacctggc tggattgcgg cggcaggacg atgtgttcgc cgttcgagat
     1921 gatcgaggag ggcggtttca aaaagatgag cggcactttg ggaacttcgt tgcccagttc
     1981 cttggcgtgt tccacgtagt tgcgccccac gcataagatc ttgctcggta cgcagggggc
     2041 ggccagtttt aattctccaa cgggaatctt tgcctcgcgg cggcggtatt ctccgaagat
     2101 atcgccctgg atctcgccga ccttatcatc aagcatccag ccgtatttga cagcctcacc
     2161 attggtttga taacgaacga ttttcatgct tctcctgtgt caaaacgaaa tctacaaagg
     2221 aaaccctttg tgctgctcac cgcctttggg tgcggtgcaa gtgttttgca aaacaaaaat
     2281 gcaggacaaa ctgggtttgc cctgcatcta ttatacaagc caacggcgat ttagtgattg
     2341 gaggaggatg gcggcttcgg cagctgcgga atgatccaat tgatcaatgc ctgcggcgag
     2401 cgggtctgga tgtagatttt gcccgggccg ctcaactcga tcacaaaccc ttcgccgcta
     2461 aacagcgtgg atttgagtcc gccgatgggt ttgggggtga cacccatgtt cccgtcaaac
     2521 gccacgagat gagaggtatc caccgtgtat ttttcgcctg cattcaggtt tttttcgtag
     2581 atcgcgccgt aggaagagac caacaacgta cccgttcccg tggcttcaag catggaaacg
     2641 ccttcggcag acatcaaggc tttcacgctc actttggcgg tcaattccac cgttgattca
     2701 gaggccatgt aggaacccga ttgaatcatc aattttccgc cgctcatctc gatgacggtg
     2761 atatcaccgg gtaactccgg ggcaagggtc acttcgccgc cctgtggggg agctacgaag
     2821 aaattctgga agaaggattc gccgcccaac acggcacgtc cgagcgattt gaggaatccg
     2881 ccttcagcct tggtttcgat ctgcacacct tccgacatgc tgaccatggc cccagcctcg
     2941 gcgcggatgc gttcattggg ggtgagtttt gcaattgcaa gagaatacga cgggcggtga
     3001 acgacttcaa tatccatgtg taaatctcct ttgggttttg ttgtagcgcg acatgttagg
     3061 tcgcgccacc tatgacgctc tacaagatca ttttacaatt tttatctcga tccgttccgc
     3121 atcatccagc aattcactga cagatttatg cagaacagga tggatcaaat ccggggcgat
     3181 ctctgccagc ggtaaaagca caaaggcgcg ttcatgcagg cgcggatgcg gcaccaccag
     3241 cgggggcgtg tagatcacca tgtcatcgaa gaacaggatg tcaatgtcga tcaggcgcgg
     3301 gccatttgta aagttgggca cacgtcccaa tgccgattca agccgtttca aatgggcgat
     3361 caatgcctct ggcttgaggt acgtctccac tttcagcacc tgattaagga aggcggcctg
     3421 ctcggtgaat ccccatggcg gcgtttcata cacagacgat ttctgcttca cggtcatttg
     3481 cggcgtgagg ttgctgatcg ctgtcttgag gttcatcatg cggttgccca tgttgcttcc
     3541 aagggcgaga taaacaatgt gattcatgcc tgccttgtca atctgaaaaa tggattatgg
     3601 cctgtgttcg tcgtaatgtt cggcgatatc cgccgcgagc cagccttcga tatccttgtt
     3661 cgtaaaagcc gagtcgggca atcctttcac gaatcgaacc atatcctgcc gtttgctttc
     3721 aaattgtttt tgcacttctt cgtctgaaag ctggttgtat tttacgatca attccctgtt
     3781 gaaggcatcg gtgtcacgat cttgccatgt gaaattcggg tcactcaaaa tacctttgat
     3841 gatgcgctcg ccttcctccc accagccaat ggcatgaccc agcaattcac ggaaattctc
     3901 cacaccctgt ttgctcagaa atgcagcctt gtcttcgacc ttctcaaagt cttcaatgta
     3961 tgtgccccat ccatgctcaa gcgtatccag gatcaaaaaa cggctcgaag ccaccagatg
     4021 ctcacgggcg tgattgatga aaataccatt gacccaggcc tgtacacggc gattttccca
     4081 cgcggccttg ttcatggaac gtaaatcagc cacggtcttc tggcgtgttt tttcaaaatg
     4141 cgcgaggaat tgcgcttcat tccagccttt gtactttgcc actgcttcgg cattgaatgc
     4201 atcaaaatca tatttcttgc gcgcatactc gcggtcctct gcaatggcga ggatgatgcc
     4261 catgccctct tcccaccatg ccagcacatg ggccagcata tcgcggaact gctcatagcc
     4321 ctgcgcattg acccgcttca agccttcatc ggcaggccag cgctcaaacc gctgaatata
     4381 ggttgcccat tcgatctcaa gaaaatcaag tgtgcgttgt ttgaaactgg acatgatgtt
     4441 ctccgttttt ataaatggat tcgccctgat gaaacgggcc ctactcggtc aatgcaacga
     4501 tctcggaatt tttcaaagcg tgatgcagat ccgcgctttt gaggatgacc gccgtattgc
     4561 gcatccccga cccgatggaa atttcctctt cgagcatcac gctcggatcg atcaacacct
     4621 tgacctgatt cagcaatccg aacggcccca ccgccccgat tcggtagccc gtcaccgcca
     4681 gaacctcatc ctctgaggcc atcgaaacgc gggattttcc aaggtgcttc cgcaaagtct
     4741 tccatgagat ttgcgcgggg ccagcgacca gagccattat aaattcatcc tcggccacac
     4801 ggaacaaaat gctccgcacc acctgcgaag cccgctgacc acggtcactt gccgcctgct
     4861 ccaacgagtg gacaggacgc gcgtcatcgt gccgaaagat tgtgtgcggc acaccaagtt
     4921 gttccaaagc aatactgact gggggagttt ccat
//
LOCUS       DAOQLC010000217         4949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7901_length_4949_cov_22.474663, whole genome shotgun sequence.
ACCESSION   DAOQLC010000217 DAOQLC010000000
VERSION     DAOQLC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7901_length_4949_cov_22.474663"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PL000_20485"
     CDS             <1..404
                     /locus_tag="PL000_20485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91314.1"
                     /translation="EVMALDPELSVVFTPSTDPLKISRFSWKDYIDQTNYAPPHQQEV
                     KVDPNLVLLLEDQEKPPKWEQELKDSSETPPPKAPAKKPAEKPKAKEPPKLDDKKKVA
                     KSESAEENVKPKKAKPKPAPDLEDDDEILPI"
     gene            401..1198
                     /locus_tag="PL000_20490"
     CDS             401..1198
                     /locus_tag="PL000_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91315.1"
                     /translation="MNYDDVEAAFRLMGLEPGAPLDEVKTAYKFSLQAFHPDKYPAES
                     AAQKMATEKLIAVKDGYEILTAFFAEHPTGEPPEGWRTKQSQQQERPGQSRASSEDST
                     DWQSWEKGEETKAPENDLHAWEQREVERQKEVKAGYGRDQRQKLVTYGKVALVIMCLA
                     LWSGKFSNNAYERVKRQQQANLLMEKAQYRQSTHDAASGYYKTDAQIHDEIDQEAERM
                     KAQWMQEDMGRWFGVLMLVAMSGGTVWVFRSKKAKDFIDSWVDGTSK"
     gene            1195..1770
                     /locus_tag="PL000_20495"
     CDS             1195..1770
                     /locus_tag="PL000_20495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91316.1"
                     /translation="MKLPELKATGVMIGSCVAAFFVFFAGYNIFHAICNDWTISTALA
                     GIAATFAFRLIPVLYISRDPAIFRPSARPYKLTPMYALTEVKDAISTQYFGERRWHLE
                     NVNPENLSLFYVCKFVQEMGQGEATTRVETVVTMKVQVTAIGDLCSVQLDFDTIAGGV
                     SQQINDLCSQTTEYIEARLVSLQNRLGGTQN"
     gene            1777..2418
                     /locus_tag="PL000_20500"
     CDS             1777..2418
                     /locus_tag="PL000_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91317.1"
                     /translation="MKVKVAKAIAAVALAVALQVPALAIPQRDQAILSGIAKEMFAKD
                     TLSVRGQKNALKKEGRPLGLYSATPAIVFRSGYQDLPSQTQAEWAYDLHDFVVARGGG
                     LENYAKYVEEQAERLKRPQIPAYRTFSKELPDPYDPLERCYVSFADRKKFADEWWSVR
                     DEGYNTALAKDWWLNDAILDGTNLWHRWEMTYLRSADREAIRTAELNQLVKKK"
     gene            2799..3926
                     /locus_tag="PL000_20505"
     CDS             2799..3926
                     /locus_tag="PL000_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91318.1"
                     /translation="MKKKLVGCYLAILLAISATVYYHYLPHHKRSVDPDASTDFIFCA
                     GDTSVDGFRLKRWTPAEQEEVCEYFRKIKKQYPKFYKYAIQRPLRLGRISKIEGRYPG
                     FKVNCWSVANAIIFPDNFFGITPEFGLSANQLKLTIVIHELTHILDHHFDLAYRKEWI
                     ELVQEREKCMWTSPENHIYSEYAKVSRCEALPSYVELYVNNFLGGECDKGVRDYLRSH
                     LFETFQACDQDLLQAEDEICSGNNRKAIQHLTRSIEKGSGNIRSYVERSILFSAENNT
                     EQERRDLRHVFFVLEKRGIAFKTDGFYKTFWRKYASLCLSDEFIDSEINRLRKLSLID
                     PEKVKKEASKLRNKFNKENPFKAAFYDWVCDPEFLEMVNSK"
     gene            4111..>4949
                     /locus_tag="PL000_20510"
     CDS             4111..>4949
                     /locus_tag="PL000_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91319.1"
                     /translation="MSDAKAKDQGDQHQVKSEVQPIHSPDAETVRLAGRVDMDRVEQA
                     RSNASDGSLRHYQATTQVMRNLPVDWHEQNQSIELIDGDVSVSRTNPLKEANLATGHN
                     ADGTPYKVYTAQASQALEKPGEIATALTQGDYQSSTKLSVQITNVPEVSTGIKPEQAL
                     GYVGQVMKAGAKAVRPVEEHLSKPNVVNQDLWNLPGNIGKVMEHLSQSPEQLDKDVAA
                     ITGQVLDTIDKPMTAERRAGAAGALMPMFFFEGGGKPIDKAAANQMKLDQMTAEELKM
                     LGI"
BASE COUNT         1478 a         1053 c         1339 g         1079 t
ORIGIN      
        1 cggaagtaat ggcgctagat ccagagctat cggtagtatt tacgccatcg acagatcccc
       61 tcaagataag ccggtttagc tggaaggact atattgacca gacaaactac gcaccaccgc
      121 atcaacaaga ggtgaaggta gaccctaacc tggtgctgct tttggaggac caggagaagc
      181 cgccgaagtg ggagcaagaa ttaaaagatt cgtctgagac cccaccgcca aaagccccgg
      241 cgaagaagcc cgcagaaaag ccaaaggcga aagagccgcc gaagctggac gacaagaaga
      301 aggttgcaaa gtccgagagc gcagaggaaa atgtgaagcc gaagaaagcc aagccgaagc
      361 cagcgccgga tctagaagac gatgacgaga ttttgccaat atgaactatg acgacgtaga
      421 agccgcgttc aggctcatgg gtttggagcc aggggcgccc ctggatgagg taaagacggc
      481 ttataaattt agcctgcaag ccttccaccc ggacaagtac ccggcagagt cggcagcgca
      541 gaaaatggcg acagagaagc tgatagcagt caaagacggg tacgaaatac tgaccgcgtt
      601 ttttgccgag cacccaaccg gcgagccgcc cgagggctgg agaactaaac agagccaaca
      661 gcaagagcgc ccaggacaaa gtagggcaag ctctgaggac tccacagact ggcagtcttg
      721 ggaaaagggc gaagagacaa aggcgcctga aaatgactta catgcctggg agcagcggga
      781 agtagagcgg cagaaagaag tcaaagcagg ctacggcaga gaccagcgcc agaagcttgt
      841 cacttacgga aaagtggcgc tggtgataat gtgtctggct ctctggtctg gcaaattcag
      901 taacaacgca tacgagagag tgaagaggca gcagcaagct aacttactca tggaaaaggc
      961 gcagtacagg caaagtaccc acgatgcagc aagcggctac tacaagacag atgcgcaaat
     1021 acatgacgag atagaccaag aagcagagcg catgaaagcg caatggatgc aggaagacat
     1081 ggggcgctgg ttcggtgtgc tcatgctcgt agcgatgtcg ggcggtactg tctgggtgtt
     1141 tcggtcgaag aaagccaagg actttattga ctcttgggtg gatggtacta gcaaatgaaa
     1201 ctgccggaac tcaaagccac tggggtgatg atagggtcat gcgtggctgc atttttcgtg
     1261 ttttttgctg gctacaacat cttccatgct atttgcaatg actggacaat atcgacagca
     1321 ctagctggaa tagcggccac gtttgccttt aggctgatac cggttctgta catatcgagg
     1381 gacccggcga tattcagacc gtcggcaaga ccgtacaaac tgacaccgat gtacgccttg
     1441 accgaagtca aagacgccat atctacgcag tatttcggag aaaggcggtg gcatctggag
     1501 aacgtgaacc cggaaaatct gagcctcttt tatgtatgca agttcgtgca ggaaatgggg
     1561 cagggagagg ctacaacgag agtagagact gtagtaacga tgaaagtaca ggtaacggca
     1621 attggcgatt tgtgctctgt acagctagat ttcgacacaa tagcgggcgg agtaagccag
     1681 cagataaatg acctttgcag ccagacaacc gagtacatag aagcgcggct ggtgagcttg
     1741 cagaatagat taggtggaac acaaaactag gtgaggatga aagtgaaagt agcaaaagca
     1801 atagcggcgg tggctctcgc agtagcctta caggtgccag ccctggccat tccgcagaga
     1861 gatcaagcga tcttgagcgg catagctaaa gagatgtttg ccaaagacac cctatcagta
     1921 aggggacaga aaaacgccct caagaaggaa ggtagaccat tggggctgta ttcggctact
     1981 cctgccatag tgtttagatc cggctatcaa gatttgccaa gccaaacaca agcagagtgg
     2041 gcctatgact tgcatgactt tgtggtagcc aggggtggcg ggctagagaa ctacgccaag
     2101 tatgtagaag agcaagcgga gaggctaaag agaccgcaga tacccgctta taggacgttt
     2161 tcaaaagaac taccggaccc atatgatcca ctggagcgtt gctacgtctc atttgcagac
     2221 cggaaaaaat ttgctgatga gtggtggtcg gtgagagatg aggggtacaa cacggctcta
     2281 gcaaaggatt ggtggttgaa tgacgctatt cttgatggga caaacttgtg gcaccgctgg
     2341 gagatgacct atctaagaag cgcagacaga gaggcgataa ggacagcgga gctaaaccaa
     2401 ttggtgaaga agaagtaaaa ccggccctgg gctggtcgaa gcggaagggc ttgctgcggc
     2461 ttacttgcgt gccttcgccg cccagggagc agtcaataga gaaagccgag ggatcgtaac
     2521 cgcccccgct ccaccgctac atctaagcat tcattgccaa attcaccata attttacgtt
     2581 ctttcgatta ttgttggctt tgaatctgta agtttcaaaa tgaaacggca ttggttctgc
     2641 cgaactggtc ttttgccttg tccccctggg tggcttttag cttaccgttt cgttggaaag
     2701 ttcaatatat cgccgtttgg agtctaaatt tggaaaaact gcaacaaata gtctaggact
     2761 ttacacctct ctaaaagaag tacgagtaaa agttcgaaat gaaaaaaaag cttgttggtt
     2821 gttatctggc tattttgtta gcaatcagtg caacagttta ttaccactac ttgcctcacc
     2881 ataaacgttc tgtcgatcct gacgctagca cggatttcat attttgtgct ggtgatactt
     2941 cagtggacgg atttaggctc aaaagatgga ccccggctga gcaggaagag gtatgtgaat
     3001 attttcgtaa aatcaaaaaa cagtatccaa aattctataa atatgccatt cagagaccgt
     3061 tgcggctggg cagaatttct aaaatagaag gaaggtatcc tggattcaag gttaattgtt
     3121 ggagcgtggc taatgccata atttttcctg ataatttttt cgggatcact cctgaatttg
     3181 ggcttagtgc aaaccagctc aagttaacta tagtaattca tgagctgact cacattttag
     3241 atcatcactt tgatttggct tacagaaagg agtggataga gttagttcaa gagcgtgaaa
     3301 aatgtatgtg gacttcgcca gaaaatcaca tttacagcga gtacgcaaag gtatcgcgtt
     3361 gcgaagctct tcccagttac gttgaacttt acgtaaataa tttccttggg ggcgagtgtg
     3421 ataaaggtgt tagagactat ttgcgttcgc atttatttga gactttccaa gcgtgcgacc
     3481 aagatctttt acaagctgaa gatgaaatat gcagtggaaa taatcgcaaa gctatccaac
     3541 atctgaccag aagtatcgaa aaaggcagcg gaaatattcg ttcttatgtg gagcgttcaa
     3601 tactttttag tgcagaaaac aacactgagc aagagcgtcg tgatttgagg catgtttttt
     3661 ttgtccttga gaagcgagga atcgccttta agacagatgg attttacaaa acattttggc
     3721 gtaaatatgc ctctctttgt ttgtcagacg agttcataga ctctgaaatt aacagacttc
     3781 ggaagctttc gctaatagac cctgaaaaag tcaaaaaaga ggctagtaag ctgaggaata
     3841 aatttaacaa agagaaccca ttcaaagctg ccttttatga ctgggtatgt gaccctgaat
     3901 tccttgaaat ggttaattca aagtagtttg tgacaaggag cttcgaagcg gacggactcg
     3961 ctacggctta cttgcgtgcc ttcgccgccc aaggagcagt aaattagagt caaccgaagt
     4021 ttcgtaaccc ccagctccac cgcttagagc tagtttctta tcagccgtac aagggcataa
     4081 tataaccaag tcgggcccca gggtgcgcga atgtcagacg ccaaagctaa ggaccagggc
     4141 gaccagcacc aggtcaaatc agaagtacag cccatacact caccagatgc cgaaaccgtg
     4201 agattggctg gtcgcgtgga catggacaga gtagagcaag cccgtagtaa tgctagtgat
     4261 gggtcactta ggcactatca agcaacaacg caagtaatgc gaaacctgcc ggtagactgg
     4321 catgaacaaa atcagagtat tgagctaata gacggtgatg tgtctgtttc gagaacaaat
     4381 ccgctcaaag aggctaatct agcaactggc cataatgctg atggaacgcc gtacaaggtc
     4441 tatacagcgc aagcatcgca agcgttagaa aagcctggtg aaatagccac agcgcttact
     4501 cagggcgatt atcagtcaag cactaagtta agtgtgcaaa taacgaatgt gccggaagtt
     4561 tcgacaggga ttaagccgga acaggcatta ggctatgtcg gccaggtgat gaaagcgggg
     4621 gctaaagcag ttaggcccgt cgaagagcac ctaagtaagc caaatgtagt caatcaagat
     4681 ttgtggaact tgccaggaaa tatcggcaaa gtgatggagc atctttcgca gagcccggag
     4741 cagcttgata aagacgtggc ggcaataacg ggacaagtct tggacacaat agacaagccg
     4801 atgacagcgg agcggagagc tggggcggct ggtgcgctca tgccgatgtt tttctttgag
     4861 ggtggcggca agccgataga caaagcagca gcaaatcaaa tgaagctcga tcagatgaca
     4921 gccgaagagc ttaaaatgct tggcataga
//
LOCUS       DAOQLC010000218         4918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_7988_length_4918_cov_21.748920, whole genome shotgun sequence.
ACCESSION   DAOQLC010000218 DAOQLC010000000
VERSION     DAOQLC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7988_length_4918_cov_21.748920"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PL000_20515"
     CDS             complement(<1..430)
                     /locus_tag="PL000_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91320.1"
                     /translation="MSFVTKVKQYPKIGLVILSFVAFVALGMPDGLLGVAWPSIRSGF
                     SIPLDALGMFLTALVVGYMTSSFFSGPIVTRLGVGKVLAISCTLTGVALFGYTFVPEW
                     WMMVLLGVVAGFGAGAIDAGLNTYVAAHFSEGLMQWLHASW"
     gene            complement(498..1511)
                     /locus_tag="PL000_20520"
     CDS             complement(498..1511)
                     /locus_tag="PL000_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC91321.1"
                     /translation="MNKKNAKTLDDIARLAKVSKSTVSRALNNSPLLNQQTKERIQTL
                     ARKHNFRVNVSARNLRIRQSHTIAFVTPDCEPNFFSPESLFGFEILGGIGNELHSLGY
                     DLLIANVNPMDTTPLNSYIGSGRADGFILLSSKNSQAIIDKMIELDAPFITWGLPLPN
                     FNYCSVDGDNITGGTLATNHLIQNGRQHIAFLGGPPEELTVQHRYQGYENAFQANGQR
                     VAAKRVVYGDYSYTSGLDAMQRLLKQSPDLDAVFVNSDLMAIAAIKVILESGRRVPED
                     IAVVGYDDVSIAMYNNLPLTTIRQNVPMAGSLLAQNLIRHIKTGEITNVTTPVELVIR
                     KSA"
     gene            1677..2537
                     /locus_tag="PL000_20525"
     CDS             1677..2537
                     /locus_tag="PL000_20525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC91322.1"
                     /translation="MEERLQKLLAQAGYGSRRHCEEFIIAGRVRVNGQVATLGQKADL
                     SVDKVTLDGKALPKAESLAYTYIALYKPRNVLSAAEGQDDRETVRDLIPLEGHLYPVG
                     RLDFDSEGLILMTNDGELTNKLTHPRYGHEKEYRVLLARRPDEEQLSAWRRGVVLEDG
                     DKTAPADVNFLSTSGKGAWIRVVMGEGKKRQIREVGKLLGLPVVKIIRLRIGTLKLGT
                     LKPRQWRYLTEDEIADLKGAKREPKPAFAKPDRRSDPKKSWARNKDSKDKPPRALQRT
                     PGKPVRKSSR"
     gene            complement(2603..3226)
                     /locus_tag="PL000_20530"
     CDS             complement(2603..3226)
                     /locus_tag="PL000_20530"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HNC91323.1"
                     /translation="MSSPVISVSPETPIHDALVMFKKEHIRRAPVIKDGKLLGIVSER
                     DLLNASPSPVTSLSVWEMNYLMSKVTVKQVMTKKVKCLDANTPIEEAARLMADSKIGG
                     MPVMRSGMIVGMITETDLFKIFLELMGAREKAIRVSALVEDKPGQLAKVTKAIADAGG
                     NFISFGIFSGPDTSSKLLTFKVAGMKKDAVKATLEKVVKKFWDIRQS"
     gene            complement(3464..4870)
                     /locus_tag="PL000_20535"
     CDS             complement(3464..4870)
                     /locus_tag="PL000_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91324.1"
                     /translation="MSKKFFTVLLLAVFMLAQFSVASAATVAGDASITNIPDFANLTD
                     VDVDWSVTGMAAGPKYILIWSRIQADPPVALYTGCMDIATINGSSDASGTFTYDLTLI
                     GPNNGDVVEFIVTVDSTACAGVPDIPVDTDAPMGDTTIDSFAPEYFNANPPSITGLPK
                     TIDPVACNTFEYWAMATDHYMFPSTIGYSGIGAWNLQVTGTFAPPAPVGPGEDLLSWK
                     FTFPSTASGAWTVSHQVEDQAGNQNGAPAYFRNALVIAPAELEDCTTFSDVSGHEYEV
                     YIRYLATLGLVSGFDDGTFGPDTTLTRAEAATLFEISNGYDATLLPTTAPAGCDFTDV
                     VASDWFAGWVWQACSDGFMSGIGDGRFDPNNLLTRGQIVTIFNNVANVISSNPGVIGG
                     YMDTDNTLAIAYPTALVLRTAAWMDVPIGAYYAEPVQLAYGWGVADGTSDTTFSPDQP
                     ATRGEYIKMLYRALSIVN"
BASE COUNT         1271 a         1158 c         1302 g         1187 t
ORIGIN      
        1 cccaactggc atggagccat tgcatcagcc cttcgctgaa atgcgccgcg acgtacgtat
       61 tcaaacctgc atcgatcgca cctgcaccaa acccggcgac cacacccaaa agcaccatca
      121 tccaccattc tggaacaaag gtgtagccga acagcgcaac accggtcagg gtacaactga
      181 tcgccagaac cttgcctacg cccaggcgcg tcacgatggg tccactaaag aaactggagg
      241 tcatgtagcc gaccaccaga gcggttaaga acatgccgag tgcatccagc ggaatggaaa
      301 atccgctgcg aatggaaggc catgctacac ctagtaagcc atcgggcatg cccaaagcaa
      361 caaaggcaac gaaagacaaa atgaccaaac cgatcttggg atattgcttt acttttgtta
      421 caaagctcat gaagatattc ttcctttacg ttttgttttc tgcaatcgat tgcagctatt
      481 tacaaaaaag caatacccta ggccgattta cggatcacca attccacagg tgtggttaca
      541 ttggtgatct cacctgtttt gatatgccgt attaaattct gcgccaacaa actacctgcc
      601 atgggaacgt tttgtcgaat tgttgtcaat ggcagattgt tgtacatggc aatggataca
      661 tcgtcataac caaccactgc aatatcttca ggaacgcgtc gtccactttc gagaatgacc
      721 ttgatcgcgg caattgccat cagatcactg tttacaaaaa cagcatcaag gtcaggggat
      781 tgtttcaaaa ggcgttgcat cgcatccaaa cccgaagtgt aagaataatc tccgtagacc
      841 acccgttttg cggccactcg ttgtccattt gcttgaaatg cattttcgta tccttgataa
      901 cggtgttgca cggtcagctc ttcgggaggg ccgcccagaa atgcaatatg ctgcctgccg
      961 ttctggataa ggtggtttgt tgcaagcgtt ccaccagtaa tgttatcccc atcaacggaa
     1021 caataattga aatttggaag tggcaatccc catgtgatga agggtgcatc cagttcgatc
     1081 attttgtcga tgatggcttg gctgtttttt gatgacaata gaataaaccc atctgcccgc
     1141 ccactgccaa tgtaggagtt gaggggagtt gtatccattg gattaacgtt tgcaatcaag
     1201 aggtcgtatc ctagtgagtg cagttcattt ccgattccac ccaatatctc gaagccgaac
     1261 agactttcag gggaaaagaa gttcggctcg caatcgggtg ttacaaacgc aatggtatga
     1321 ctttggcgta ttcgaaggtt gcgtgcagaa acattgacac ggaaattatg tttcctggca
     1381 agtgtctgga tgcgttcttt tgtttgttgg ttaaggaggg gactgttgtt taaagcacga
     1441 gaaactgttg acttggaaac tttcgccaac cgggcaatgt cgtcaagggt ttttgcgttt
     1501 tttttgttca cgggtgactt tctttgcaat cgattgcatt atagcactgt tcgctgttgt
     1561 atcggctcaa gaatttgaag gtcagaggcc tatttgtaca ggtgtgtatc ggtgttcgat
     1621 ttagattcat tcgaaccgcc gtgcccgtca ctgaaatccg ttataatgct gtctctatgg
     1681 aagaacgatt acaaaaacta cttgctcagg ctgggtatgg atctcgccgc cattgtgaag
     1741 aatttattat tgcaggacgt gtgcgggtca atgggcaggt ggccactctt ggccaaaagg
     1801 ccgatttatc tgtcgacaag gttactctgg atggcaaggc tttacccaaa gccgaatcat
     1861 tggcgtatac ctacattgcg ctttacaaac cccgaaatgt actttctgca gcagaaggtc
     1921 aggatgaccg cgaaacagtt cgcgatttga ttcccctgga aggccattta tatcccgtcg
     1981 gtcgcctgga ttttgattca gaaggtctga tcttgatgac caatgacggt gaactgacca
     2041 acaaacttac acacccccgc tatgggcatg aaaaagagta ccgtgttttg cttgctcgta
     2101 gacctgatga agagcaactt tcagcatggc gtcgcggagt tgtcttggaa gatggcgata
     2161 agaccgcccc tgccgatgta aatttcttat ccacctctgg gaagggtgcg tggattcgag
     2221 tggtcatggg ggaagggaaa aagcgccaga tccgtgaggt ggggaaactg ctgggcctgc
     2281 ccgtggtgaa gatcatccgt ttgcggattg gaacattgaa acttggcaca cttaaacccc
     2341 gccaatggag atatctgacc gaagacgaaa ttgccgacct gaaaggcgca aaacgcgagc
     2401 cgaaacctgc ttttgcaaaa cctgacaggc ggtcagaccc gaagaaaagc tgggcaagaa
     2461 ataaagactc gaaggataaa ccgccccgcg cccttcagcg gacacccggg aagcccgttc
     2521 gaaaatcttc cagataaaaa aacgaaaacc acccgcaatc agtattcacg ggtggtttta
     2581 cttttaacct ggtttcagaa aattagcttt ggcggatatc ccagaacttc ttgaccactt
     2641 tttcaagcgt ggctttgaca gcatcttttt tcatgccagc cactttgaag gtcaacagct
     2701 tcgagcttgt gtctgggcca gagaaaatac cgaaggaaat gaagtttccg cctgcatcgg
     2761 caatggcttt ggtcaccttt gccagttgac cgggtttatc ttccaccaag gcactgacgc
     2821 ggatggcctt ttcgcgggcg cccatcagct caaggaagat cttaaagagg tcggtttcgg
     2881 tgatcatacc cacgatcatg ccagaccgca tgacgggcat gccgccgatt tttgaatcag
     2941 ccatcaagcg ggcggcttct tcaatggggg tgttcgcatc caggcactta actttcttgg
     3001 tcattacctg tttgacagtg actttgctca tcaggtagtt catttcccat acgctcaggc
     3061 tggtgacagg ggagggagat gcgttgagca aatcgcgctc agaaacaata ccaagtaatt
     3121 ttccatcttt gatgaccgga gcacggcgga tgtgttcctt cttgaacatc accagcgcat
     3181 catggatcgg ggtttcgggg gagactgaaa taacagggga cgacattctt tcgccgacaa
     3241 acatttttac ctccagggta gtaaaaggac gtgttgtgaa atttgttaca aacacattat
     3301 aagaggattt cgggaaaaga aagtcactaa attggagtga ataaggtcac tttgcggtgg
     3361 cgcatggatg tcatatctca atttgttggg gtgatttctg cgttaaaaca aaacgggcga
     3421 acccaaattg gattcgcccg tcaacctaac ttgattcaga aacttagttc acgatgctca
     3481 gagcgcggta caacatcttg atgtattcgc cgcgggtggc gggttggtcg ggtgagaagg
     3541 tggtgtcgga ggtgccatca gcaacgcccc agccatacgc cagttgaaca ggttcggcat
     3601 agtaggcgcc aatgggaaca tccatccatg cagcggtgcg caaaacaagg gctgttgggt
     3661 aagcaatggc aagggtgtta tcggtatcca tgtatccacc gatgacgccg ggattcgagg
     3721 aaattacatt cgcaacatta ttgaaaatgg tgacgatctg accacgggtg agcaggttgt
     3781 tcgggtcaaa tctgccatcg ccaatgccgc tcatgaatcc atcagaacag gcttgccaga
     3841 cccaaccagc gaaccagtcg cttgcgacta catcggtgaa gtcacagcca gcaggggcgg
     3901 tggtgggaag caaggtggca tcgtagccgt tggaaatttc gaaaagggtg gcggcttcgg
     3961 cgcgggtgag ggtggtatcg gggccgaagg tgccatcatc gaaaccactg accaggccca
     4021 gggtggcgag gtagcggatg tacacttcat attcgtggcc gcttacatca gagaaggttg
     4081 tgcaatcttc caattctgcg ggtgcgatga caagggcatt gcgaaaatag gccggagcac
     4141 cgttttgatt tccagcctgg tcttccactt gatgagacac tgtccatgca ccgctggcag
     4201 ttgaagggaa tgtgaatttc caagagagca ggtcttcgcc agggccaaca ggagcaggcg
     4261 gggcaaaggt gccggtaacc tgaagattcc aagctccgat cccagaatat ccaatcgtgg
     4321 atgggaacat gtagtggtca gtagccatgg cccaatattc gaaggtattg caggcgacag
     4381 ggtcaatggt ctttggcaga cctgtgatgc tgggcgggtt ggcgttgaaa tactccggcg
     4441 caaaagagtc gatagtggtg tcgcccatgg gtgcatcggt gtcaacgggg atgtcaggca
     4501 cgccagcaca ggcagtgcta tccacggtga cgatgaattc caccacatcg ccgttattgg
     4561 gaccaatgag ggtcaagtca taggtgaagg tgccgctcgc atcgctggag ccattgatag
     4621 tggcgatatc catacaaccg gtgtagagag cgaccggggg atccgcctgg atgcggctcc
     4681 agatcaaaat gtacttgggg ccggccgcca tgccagtcac cgaccagtcc acatccacgt
     4741 cagtgagatt ggcgaaatca gggatgttgg tgatggaggc atcgccagct acagtggctg
     4801 cggaggccac actgaattgg gccagcatga acacagccaa aagcaaaaca gtgaaaaatt
     4861 ttttactcat tcgtttctcc tcgaaaaatg aatttcaaac taaattggtc tgaatacg
//
LOCUS       DAOQLC010000219         4906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8014_length_4906_cov_18.589569, whole genome shotgun sequence.
ACCESSION   DAOQLC010000219 DAOQLC010000000
VERSION     DAOQLC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8014_length_4906_cov_18.589569"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PL000_20540"
     CDS             complement(<1..190)
                     /locus_tag="PL000_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91325.1"
                     /translation="MNKYIKTLLVVMIFGLSACGQSRTTPQQDVFLDPPAIVVKASRE
                     NCPSIVAEVGMTIAWENVD"
     gene            complement(274..2538)
                     /locus_tag="PL000_20545"
     CDS             complement(274..2538)
                     /locus_tag="PL000_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91326.1"
                     /translation="MSDFNPSPEFEEKIRKATDVPGARPEFVNKLRNELARRPVKMKP
                     RFILKPAWAFAFVIVALALIASAPAAVNALKKLFGYVPGVGLVDNSSGIRVLAEPASV
                     TREGVTLTVTQVLVYPDHVQLIYEVSGIATENDGNFASDAAQNFTAFCGSPDGDAKLR
                     LPDGTIIERAFDTEKYPENIFATKPAYETVIPADMMEMTMVLKCLPWARLGAVPENWE
                     VPLKLKYVPAGETIGEPVLDVTPSASSAASDQGLSVTLDKVVPQDDLYSFYLTIAPEQ
                     ADESVLAYFPTYATLVDATGQKISLVYMHPFSGSHDVAEPWELQSITRPAYGPYTLVL
                     DSVATYYNEDGSHFFDVDLGPNPQVGQTWEINQTLNLAGTDVRVLSATMAERDLSMWG
                     GAANTQGLVFTFEAVDGKTPFSLDIMDRDVDHIMNGSIFPFSDGLTNTSEQYVTGLFY
                     EKGIPSGKLSIAVNGQTTMTNGHWEFQWASPDQTGEELLSASESISSAAGSSGVTTEL
                     RRVVKTNDGYLFFVHMTTPEQNPDFRVVEPVEVSVVDSTGKKIKLNLDGPQVYSARDE
                     NLWQFSTAEQVAAGPLKLVVDKAKVYYSKFNFATPPPADVYQNLVKENSFIFDAGADP
                     QVGQEWMLDQEFEAGGYHGIVKSVRAVTVDSQMLPFPELQPDASLNRGYEFTVEAMDP
                     SIQWNVSLFVSKPEGDPGFADCIGGMDGEAGSVTTHTMSCRGLSSGILQVEISEISIW
                     LNEIWEVAWVMPGQ"
     gene            complement(2541..3065)
                     /locus_tag="PL000_20550"
     CDS             complement(2541..3065)
                     /locus_tag="PL000_20550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC91327.1"
                     /translation="MTQNEISSLIFAALEDPAAFGVLYDRYVQHIYRYAYSRVENARE
                     AEDITAQTFIAAYESLARYRERGHFSAWLFRIAQSEIQNHFRRSRREVGLDAVRRLAE
                     ADDALVAVIQDEELSRLRSLIKKLDDDERELIRLRYVAELSFAEMADLLGRREDAVKK
                     SVYRLLERLKGQME"
     gene            3358..4680
                     /locus_tag="PL000_20555"
     CDS             3358..4680
                     /locus_tag="PL000_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HNC91328.1"
                     /translation="MPIKTLIMGAAGRDFHNFNTFFRGNKDYEVVAFTATQIPDIEGR
                     VYPAELAGAQYPKGIPIRAEEELLDLIKKYGVEQVVFSYSDVPHEYVMHKAAMVNAAG
                     ADFRIMGTKNTQLKSNKPVVSISAVRTGSGKSQTTRRVALLLKEMGYKVAAIRHPMPY
                     GNLVAQEVQRYASYDDLDEFDCTIEEREEYEPHIDCGVIIYAGVDYEKIIRQAEAEAD
                     IILWDGGNNDFPFYTPDLQIVIADPHRTGHESTYYPGETNVRMADVFVINKVDTADAE
                     NVIKLRENLRKLNPNATQIEAASPLFVDDPDAIQGKRVLVIEDGPTLTHGEMAYGAGY
                     IAARRFGAKEIVDPRPFAVKSIAATYAKYPKTGPILPAMGYGDAQTKDLEATINASDV
                     DLVVIGTPIDLSRVMKITKPHQRVRYELQEIGVPTLKDVLMKKFGAKK"
     gene            complement(4717..>4906)
                     /locus_tag="PL000_20560"
     CDS             complement(4717..>4906)
                     /locus_tag="PL000_20560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91329.1"
                     /translation="FFLFVGCFIEKIITNEIKKRGSRMIFITRLPDLKSFVFGPKLLH
                     QHIKKMLSTASPPPRTAA"
BASE COUNT         1276 a         1318 c         1156 g         1156 t
ORIGIN      
        1 tatcgacatt ttcccaggct atggtcatcc caacctcagc tactatggaa ggacagtttt
       61 cccgtgaagc tttcactaca atggcaggtg ggtcgagaaa aacatcctgt tgcggcgtgg
      121 tgcgtgattg tccacaggca gaaagtccaa aaatcattac cacaagcaaa gtctttatat
      181 atttattcat tatttttcct tcggccgaca tcgttgtatc aagattgaac tcttgataca
      241 acgatgttta cgaaaaaatt gttccccgtt ggcctactga ccgggcatca cccaagccac
      301 ttcccaaatt tcattcagcc agatcgagat ctcagaaatt tcaacctgta aaattccgct
      361 ggaaagtcct ctgcaactca tggtatgtgt ggttacagaa cctgcttctc catccatgcc
      421 tcctatgcag tcggcaaatc cgggatcgcc ttcgggtttg gaaacgaaca aactcacatt
      481 ccactggatg gacggatcca tcgcttcgac cgtgaactca taccctctgt tgaggctggc
      541 atcgggttga agttcgggga aaggcagcat ttgcgagtca accgtcacag cacggacaga
      601 tttcacgatc ccgtgatatc cgccagcttc aaactcttga tccaacatcc actcttgacc
      661 cacttgcgga tcggcgcctg catcgaaaat aaaggagttt tccttgacga gattttgata
      721 tacatcagca ggcggagggg tcgcaaaatt gaatttggaa tagtaaacct tcgccttgtc
      781 cacaacgagc ttgaggggac cggcggcaac ctgctccgct gtgctaaact gccatagatt
      841 ttcatccctt gcgctgtaaa cctgcggacc gtccaaattg agttttatct ttttcccggt
      901 cgagtcgacc acggagactt caaccggctc aacgactctg aaatccgggt tttgctcagg
      961 tgttgtcatg tgaacgaaga ataggtaccc atcattggtt ttgaccacgc ggcgtaattc
     1021 ggttgtcaca ccagaggaac cggccgcaga ggagattgat tccgaagcgc tgagcaactc
     1081 ctcccccgtc tgatccggcg acgcccactg aaattcccag tgaccgtttg tcatggttgt
     1141 ctgcccgttg acagcgatgg ataattttcc agacggaatt cccttttcat agaaaagccc
     1201 ggtcacatat tgttctgaag tattagtcag accatcactg aacgggaaga tcgagccgtt
     1261 cataatatgg tcaacatcac gatccatgat atccagcgag aatggcgtct tcccatccac
     1321 agcttcgaag gtgaacacaa gtccctgcgt gtttgcagcc ccgccccaca tggacaggtc
     1381 tctctccgcc atcgtcgccg aaaggacgcg cacatccgtg ccagccaagt tcaaggtttg
     1441 gttgatctcc caagtctgcc ccacttgcgg attcggtccc aaatcaacat cgaagaaatg
     1501 acttccatct tcattgtagt aggtcgcaac agaatcgagt accagagtgt aggggccgta
     1561 ggcgggcctg gtaattgact ggagttccca cggttcagcc acatcatgcg agccagagaa
     1621 aggatgcata tagaccaaag agatcttctg tccggtcgca tccaccagtg tggcgtaagt
     1681 ggggaaatat gccagcacag attcgtctgc ctgttcgggc gcaatggtga gataaaaact
     1741 ataaagatca tcctgtggca caaccttatc caaagtgaca ctcaatccct gatcagaggc
     1801 tgcgctcgaa gcggaaggcg tcacatccaa cacaggctcg ccgattgtct caccagcggg
     1861 aacgtatttc aatttcaacg gcacttccca attttcagga accgcaccaa gtcgcgccca
     1921 cggaaggcac ttgagtacca tcgtcatttc catcatatcc gctgggatga ctgtttcata
     1981 cgcgggtttg gtcgcaaaaa tattttcagg atatttttct gtgtcaaaag cacgctcgat
     2041 gatcgtgcca tcaggcaggc gcaatttggc atcaccatct ggcgaaccac aaaacgcggt
     2101 aaagttttgg gctgcatctg atgcgaagtt accatcattc tccgtcgcga tgccgctgac
     2161 ttcatagatc aactgcacgt ggtcaggata aaccaatacc tgtgtcacgg tcagcgtcac
     2221 accctctcgc gttactgaag caggttccgc taaaacccgt attccgctgc tgttatccac
     2281 caacccaaca ccgggcacat acccaaacaa ttttttcaaa gcattcactg cggcaggcgc
     2341 gctggcaatc aatgcaagcg ccacgatcac aaaggcaaac gcccatgcag gtttcaatat
     2401 aaaacggggt ttcattttca ctggcctcct cgccagttca ttacgcaact tattgacaaa
     2461 ttcagggcgc gcacccggca catccgtcgc tttgcgaatc ttttcttcaa attcaggaga
     2521 gggattaaag tcgctcatat ctactccatc tgtcctttca gcctctcaag caaacgatac
     2581 accgatttct tcacggcatc ttcgcgcctc cccagcagat ctgccatttc ggcaaaagat
     2641 agttccgcca cgtatcgcaa gcggatcaac tcacgttcat cgtcatctaa ttttttgatc
     2701 agggatcgga gccgactcag ctcttcgtct tgaatgacag caaccagcgc atcatccgcc
     2761 tcagccagac gcctcaccgc atccaggccg acctcgcgcc tgcttctacg aaaatgattc
     2821 tgaatctcac tttgggcgat ccgaaacagc cacgccgaaa aatgaccgcg ctctcgatat
     2881 cgtgccaacg attcgtaggc tgcaataaaa gtctgcgcgg tgatatcttc cgcctcgcgg
     2941 gcgttttcaa ccctgctgta ggcgtagcgg taaatgtgct gaacatatcg gtcatacagc
     3001 acaccaaatg cggctgggtc ctcaagtgcg gcaaagatca aagaggaaat ttcgttttga
     3061 gtcatctgaa ttttccgatt tgcttcaaat cctatcatgc aaatcctttc tctcatgagt
     3121 gtgggtgtga atgcatccac aggttaaacg gtctcaaagc caaaaaggga caaattcccc
     3181 gccgccagtt cgaaaactgt catactaaaa acaggctgaa agttatctag tctagcggcc
     3241 tgttttcctg tttaatccac ttataagacc gcattgggta aaaacgaaac aactcaaaac
     3301 ccaccccgag ttctttcgga cagtgtcctc aacgcggatg atagatagga gacccctatg
     3361 ccaatcaaaa ccctcatcat gggagccgct ggccgcgact tccacaattt caataccttt
     3421 ttccgcggga acaaggatta tgaagtagta gccttcacag cgacgcagat ccccgatatc
     3481 gaaggccgtg tgtaccctgc cgagttggcg ggcgcacaat acccgaaagg gatcccgatc
     3541 cgcgcggaag aggaattgct tgacctcatc aagaaatacg gtgttgaaca ggttgtattt
     3601 tcatacagtg atgtgccgca cgaatacgtg atgcacaagg cagcgatggt caatgctgcg
     3661 ggcgccgatt tccgcattat gggtacaaag aacacccagc tcaagagcaa caaacccgtc
     3721 gtgtcgatca gtgccgttcg gacgggatca ggcaaaagtc agaccacgag gcgcgtggct
     3781 ttacttctca aagaaatggg atacaaagtc gctgccattc gtcatccgat gccgtatgga
     3841 aatcttgtag cgcaggaagt tcaacgctac gccagctacg atgacctcga tgaatttgat
     3901 tgcaccatcg aagaacgcga ggagtacgaa cctcacattg attgcggtgt gatcatttat
     3961 gcaggcgtgg attatgaaaa gatcatccgc caggccgaag ccgaagccga tatcattttg
     4021 tgggatggcg gcaataacga cttccccttc tacacccccg atctgcagat cgtcattgca
     4081 gacccgcatc gcacggggca cgagtcaact tattatccgg gcgaaactaa tgtgcgcatg
     4141 gcagacgtgt tcgtcatcaa caaggtggat accgcggacg ccgagaatgt gatcaaactg
     4201 cgcgagaact tgcgcaaatt gaacccgaat gcaacccaga tcgaagccgc ctctccgctc
     4261 tttgtggatg accccgatgc gattcagggc aagcgcgttt tggtgatcga agacggcccg
     4321 actctgactc acggtgagat ggcctatggc gcgggatata tcgccgcccg cagattcggc
     4381 gcgaaagaaa tcgttgaccc gcgtccgttt gcagtgaaat ccattgcagc gacctacgcc
     4441 aaatacccga agactggccc gatcctccct gctatgggct acggtgatgc acagaccaag
     4501 gacctcgaag ccacgatcaa cgcttcggat gtggacctgg tcgtcatcgg cacaccgatt
     4561 gacctgtctc gcgtgatgaa gatcacgaaa ccgcatcagc gcgtaagata tgaattacag
     4621 gagatcggcg ttcccaccct gaaagatgtt ttgatgaaga aatttggggc gaagaaataa
     4681 gagcgtagat atatttacga tcagaaagtc tactgctcaa gccgccgtcc tcggcggcgg
     4741 ggacgccgtc gagagcatct ttttaatatg ttgatgaaga agtttgggac caaatacaaa
     4801 ggattttaga tcaggaagtc gggtgatgaa gatcattcgg cttcctctct ttttaatttc
     4861 gtttgttata atcttttcga taaaacaacc aacaaaaagg aaaaag
//
LOCUS       DAOQLC010000220         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8018_length_4904_cov_21.952774, whole genome shotgun sequence.
ACCESSION   DAOQLC010000220 DAOQLC010000000
VERSION     DAOQLC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_4904_cov_21.952774"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2708)
                     /locus_tag="PL000_20565"
     CDS             complement(<1..2708)
                     /locus_tag="PL000_20565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC91330.1"
                     /translation="MSQTKPTYQELEQRCQAAESALTAIRSGQADMVIGENGVLVLRL
                     KEAEKAFQEAELKYRSLAENSPDIIYIIDLKKNKSGYLNRETLLGYSLEELEKPGESL
                     LSKVHPDDLSAVKMHWQEALAGSTTTELEYRLQLKDGSWEWLHSRPRVLSWSAEGKPV
                     QLLVTLSIITERKQAERRLRSMLDIAQAMSQFLDTKTILQQIVDNITQVAGLDSGAIY
                     TLADDQLYLEATTPALPPEFPEQFRLANLADHPHIRAALAERSPVIISDTGNAELTEA
                     EREVSVSRGLRSIAYLPLMSARKVIGVLIVASAHGLRTFSEEELNLYKGFSGQAAQII
                     ENAQLYDSIRVHSNELEQQITERKQAEEKLRESEKLYRHAMEVAGAVPYYELYNENPP
                     AVKYEFIGEGIRQITGYGPEEFNAMVWDSLVEKAVPVEELEGFSLDEAVQRTRNGEFP
                     IWKCEFRLHDRDGKVHWVFEAAVELRDENGNPVGSIGSYQDITIRKQAEEKLRKSEEQ
                     YRGLMGTLDSVVATIDDSGKFLYMNEVAASQLGGAPHQLIGRSLHELFPEPLAFQYLQ
                     GVHNVMQTNKSMVNETLTIVDGGARWFRNSLQPIHDENGNVIQVLLHAVDIHELKTIQ
                     QELADLNKSLERRVEERTVEVRQANQALERALLAKDEFLASMSHELRTPLTGILGFSE
                     AMQMNVYGILNERQQKIVGSINSSGKHLLDLINDILDLSKTEAGKLEIQKSPTLLADI
                     CLASLQLTKGMSHKKGQYVNTVIPEEPIILDLDMLRIKQVLVNLLSNAIKFTPENGEL
                     GLTVETDEANRLVRVIVWDKGIGIKPENFPKLFQPFTQIDGSLAREYVGTGLGLSLVR
                     RLVELHDGSVTVESVFGEGSRFIVTLPWITKISPSVSEHTETAWQAS"
     gene            complement(2741..3037)
                     /locus_tag="PL000_20570"
     CDS             complement(2741..3037)
                     /locus_tag="PL000_20570"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HNC91331.1"
                     /translation="MTINSQTNVRKKHYILRLFVAGNATNSRIARENLDQLLAKHPEH
                     EFEVEIVDLNLEPEVALDQGVFISPALQILEPNAGGVIYGNLSQMEVLEKVLNL"
     gene            complement(3027..4454)
                     /gene="kaiC"
                     /locus_tag="PL000_20575"
     CDS             complement(3027..4454)
                     /gene="kaiC"
                     /locus_tag="PL000_20575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HNC91332.1"
                     /translation="MNTQLLKTPTKIRGLDEILNGGVPTGGLTLLNGGPGTGKTLLGL
                     EFLIRGAKERRPGIFVTFEEHEAALHRYSASFGWDTAELERQGLLSIIGARFDPEGLL
                     SGDFDLGGILAILRHKTEKLSARQVVIDAPDVFLRLLNSPGKERAELYKLINWLRDQK
                     MTTLMTVKTNGEDVTSSDYEFLDYLADCVIQLDQRVIEQVTTRRVRVVKFRGSAHGRN
                     EYPFAMTDQGIWIIPITETQLTHTALGEPFPTGVKGLDAHMGGGYLRASCNLVTGTSG
                     TGKTTFAASFARTSTANGKRVFYINFEESPDSMVSCMLSPGIDLRPALESGFLRFLSV
                     MPEAQGIEEHLIQAFRGIEELKPEIVIVDAISACRRMGSRNSAFDYLLRLIDHCKTIG
                     ITSLLTNLTDSIKETQEITGIDLSSMIDTVIILRNVEQNGQFQRELSVLKARGRLHSN
                     KIHAFQISDHGVSVINSIAEATRDH"
     gene            complement(4677..>4904)
                     /locus_tag="PL000_20580"
     CDS             complement(4677..>4904)
                     /locus_tag="PL000_20580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91333.1"
                     /translation="LMDIQMPGMDGLMTIRHIRTHADPVVASTRIIAVTALAMAGDRE
                     RCLAAGANEYISKPVNLKELKQMIESQLGKL"
BASE COUNT         1212 a         1283 c         1129 g         1280 t
ORIGIN      
        1 gaagcctgcc atgcagtttc agtatgttcc gaaacagacg gggatatttt cgttatccat
       61 ggcaaggtaa caataaagcg gctgccttca ccgaaaacgc tttcgacggt cacacttcca
      121 tcatgaagtt ccaccaggcg gcgcaccagt gaaagcccca aacctgtgcc gacatactct
      181 cgtgccagac tgccatcgat ctgagtgaaa ggttgaaata gtttggggaa attctctggt
      241 ttgatgccaa tacctttatc ccatacgatc acacgaacca gccggttggc ttcatctgtc
      301 tcaacagtca accctaattc gccgttctcg ggggtgaact tgatggcatt gctaagcagg
      361 ttgacaagga cctgtttgat acgcagcata tccaggtcga gaataatggg ttcttcaggg
      421 atgacggtat tgacatactg tcccttttta tgggacatgc ctttggtcaa ctgcaagctc
      481 gccaggcaaa tatccgccaa caaggtgggc gatttttgaa tttccaactt gccagcctcg
      541 gttttggaaa gatcaaggat atcgttaatc aggtcgagca ggtgtttgcc gctgctgttt
      601 atacttccaa caatcttttg ctgccgttca ttcaatatac cgtagacatt catctgcatg
      661 gcttcggaga agccgagaat gccggtaagc ggcgtgcgca gttcatgact catacttgcc
      721 agaaactcgt ctttcgcaag taaagcacgt tccaatgcct gatttgcctg acgcacttcc
      781 actgtgcgct cctccacccg tcgttcaagc gatttattca ggtctgccaa ttcctgctgt
      841 atcgtcttaa gttcatgaat atcaacggca tgaagcagca cctgaattac attcccattt
      901 tcatcgtgga tcggctggag cgaattgcga aaccagcggg ctccaccatc tacaatggtg
      961 agagtttcgt taaccatgct cttgttcgtt tgcatcacgt tatgcacccc ctgtaaatat
     1021 tggaaagcca gaggttcggg gaaaagttcg tgcaacgatc taccaatcaa ttgatgtggg
     1081 gcaccgccca gttgacttgc agcgacctcg ttcatataca ggaatttgcc gctgtcatca
     1141 atggtggcaa caacgctatc cagagtaccc atcaaaccac ggtattgttc ttcactcttc
     1201 cgaagtttct cctccgcctg tttacggatg gtgatatctt gataagaacc aatcgaacct
     1261 acagggtttc cattttcgtc ccgcagttcc actgccgctt caaagaccca atgtaccttt
     1321 ccatcacgat cgtgaaggcg gaattcacac ttccagatag gaaattcacc gttacgggtt
     1381 ctttgaaccg cttcatccaa agaaaaccct tccagttcct ccaccggtac tgctttttcc
     1441 actagtgaat cccaaaccat tgcattaaat tcttcaggtc cgtagccggt gatctgacgg
     1501 atcccctctc cgataaactc atatttaaca gcaggaggat tctcgttata caactcgtaa
     1561 tagggcacag ccccagccac ctccatggca tgacgataca acttctccga ttcccgcaat
     1621 ttctcctctg cctgtttacg ttcagtaatc tgttgttcca gttcattgga atgaactcta
     1681 atggaatcat ataactgtgc attttcaatg atctgtgccg cctgaccaga gaacccctta
     1741 taaagattca attcctcctc ggaaaatgta cgcagcccat gggcggatgc cacgatcaag
     1801 accccgatca ccttcctggc actcatgagc ggtaaatagg cgattgaacg cagccccctg
     1861 gaaacgctca cctcgcgctc ggcttcggtc aactcggcgt tcccggtatc agatatgatg
     1921 acgggtgatc tttctgcgag ggctgctcga atatggggat gatcagccag atttgccaaa
     1981 cggaattgtt ctggaaattc gggcggcaag gcaggggtgg tggcctccag ataaagttgg
     2041 tcatctgcca gtgtgtagat cgctccacta tccagcccag ccacctgggt aatgttatca
     2101 acaatttgct gcaagatcgt ttttgtatca aggaactgag acatcgcctg ggcaatatcc
     2161 agcatggacc gtaaacgtct ttccgcctgt tttcgttcag taattatgct aagtgtgacc
     2221 aataactgaa ctggcttgcc ttccgcgctc caactcaaaa cacgtgggcg gctgtgaagc
     2281 cattcccaac tgccgtcttt caactgtaag cggtattcca attctgttgt ggtactgcct
     2341 gcaagagctt cctgccaatg cattttaaca gcagagagat catcaggatg caccttggat
     2401 aacagggact ctcccggttt ttccaactcc tccagagagt accccaatag agtttcgcgg
     2461 ttcaagtagc cagacttatt ttttttcagg tcaatgatgt aaataatgtc cggtgaattt
     2521 tctgccagag agcgatattt taattctgct tcctgaaagg ctttttccgc ctctttcagg
     2581 cgcaaaacca ataccccatt ctcaccaatg accatgtctg cctgtcctga gcgaatcgca
     2641 gtcagtgccg actcggcagc ttgacagcgt tgttccaatt cctgataggt cggttttgtc
     2701 tggctcaagg atcacgctcc atttccatgc aataaacaat tcaaaggttc aggacctttt
     2761 ccagaacttc catctgactt agattgccat atatgacccc gcctgcattc ggttcaagga
     2821 tttgcagggc aggtgaaatg aacacaccct gatccagggc cacctcgggt tccaggttga
     2881 gatcaacgat ttcaacttca aattcatgtt cagggtgttt tgccagcagc tgatcgaggt
     2941 tctcacgcgc gatccgtgaa ttggtggcat tccccgccac gaacaggcgc aggatgtaat
     3001 gcttttttcg aacattcgtc tgagaattaa tggtcacggg ttgcctccgc aattgaattt
     3061 attacggaaa ccccatgatc tgaaatttga aacgcatgta tcttattgga atgcaggcgt
     3121 cctcttgctt tgagcactga tagctcccgc tggaactgcc cattttgctc cacgttacgc
     3181 aaaatgatca cagtatcgat catcgaggaa aggtcaatac cggtgatctc ttgagtctct
     3241 ttgatgctgt cagtgaggtt ggtcagcagg ctggtaatac cgatggtctt gcagtgatcg
     3301 atcaggcgca atagataatc gaacgctgaa ttacgcgatc ccatccggcg gcaggctgag
     3361 atggcatcca cgatcacgat ctcgggtttt agttcttcga tcccgcggaa tgcttgaata
     3421 agatgctcct cgattccctg agcttcaggc atcacagaca ggaatcgaag gaagccgctt
     3481 tccagggcag ggcgcaaatc aatgccgggg ctgagcatgc atgacaccat cgagtcaggc
     3541 gattcttcaa agttaatgta gaaaacacgc ttgccgttcg ccgtggatgt gcgcgcaaaa
     3601 gatgcagcaa aggtggtctt gcctgtacca gatgtgccgg tgacaaggtt gcatgaggca
     3661 cgcagatacc cgccgcccat gtgcgcatcc agacctttga cgccggttgg gaacggttcg
     3721 cccaaagcag tgtgggtcaa ctgtgtttct gtaatcggga tgatccagat accctgatcg
     3781 gtcatggcaa acggatattc atttcgccca tgtgcagaac cgcggaattt gaccacacgc
     3841 acccttcggg tggtgacctg ttcgatcaca cgctggtcaa gctggatcac acaatctgcc
     3901 agatagtcga gaaattcata gtcggaactg gttacatctt ccccatttgt cttgaccgtc
     3961 ataagagtcg tcatcttttg gtcacgcagc cagttgatca gtttgtacaa ttcagctcgt
     4021 tccttgccgg ggctattgag cagacgaagg aagacatcgg gagcatcaat aaccacctgc
     4081 ctggctgaaa gtttttctgt tttatggcgc aagatcgcca agattccccc cagatcaaaa
     4141 tcaccggaaa gcagcccctc cgggtcgaac cgcgctccaa tgatcgaaag gagtccttgt
     4201 ctttccaatt cagcggtatc ccaaccgaat gaggcggaat atcggtgcag cgccgcctca
     4261 tgctcttcaa atgtgacaaa aatcccagga cgcctctctt tcgcgcctcg aatcaggaac
     4321 tccaacccaa gcaaagtctt accggtacca ggtccgccat tgagcagggt cagcccgccg
     4381 gtaggcaccc ctccattgag gatttcatca agccccctta ttttagtggg agtttttagc
     4441 aactgggtat tcataaacac ctcctgattt tatgcgcaaa cgttacaaag aaaccgtggt
     4501 ttttgccata agtaataaaa ccagtatgcc agtaatgtaa attattttat acaacttaac
     4561 caattatgtc tatcagtgaa atatctaaaa aggacaggaa aagctttgag gtcgatggcc
     4621 atacacacct aaaaatggac ccgcccacaa tgccctaact tcagagatgg aatgatttac
     4681 agctttccaa gctgagactc gatcatctgt tttagttctt tcaaattgac aggcttgctg
     4741 atgtattcat tagctcctgc ggcaaggcag cgctcccgat ccccggccat ggccagggca
     4801 gtcactgcga tgatcctggt ggatgcaaca accgggtcgg catgcgtacg gatgtggcgg
     4861 atcgtcatga gaccgtccat gccgggcatt tgaatatcca tcaa
//
LOCUS       DAOQLC010000221         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8067_length_4888_cov_24.913511, whole genome shotgun sequence.
ACCESSION   DAOQLC010000221 DAOQLC010000000
VERSION     DAOQLC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8067_length_4888_cov_24.913511"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1229
                     /gene="coaBC"
                     /locus_tag="PL000_20585"
     CDS             21..1229
                     /gene="coaBC"
                     /locus_tag="PL000_20585"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNC91334.1"
                     /translation="MSILSDKHILLGVTGSIAGYKAADLASKLAQSGAKVDVLLTEAG
                     EKFVTPLTFQSVTGRRAYTDKDLWGHEAHVLHVSFGKTADLLVIAPCTADTIAKLANG
                     IADNLLTVTALAATCPLVIAPAMDGGMFNHPATQENLAKLKERGAIIIGPAEGHLASG
                     LVGTGRMVEPVEILGQIRMVLGRRYGKLAGKKVVVTAGGTQEALDPVRVITNHSSGKQ
                     GYAIAQAALDSGADVCLVTAPTALTAPVGARVVKVNSAEEMLQAVLAESADALVMAAA
                     AADFRPKHIAKDKMKKRDGIPQIELEAAPDILKTVSGTPVEKKRFRVMVGFAAESQNL
                     LENADEKLKSKKLDFIVANDISANDAGFAVDTNRVTFLFANGARENLPLTSKMEVAEI
                     IIEHISRLLE"
     gene            1232..1963
                     /locus_tag="PL000_20590"
     CDS             1232..1963
                     /locus_tag="PL000_20590"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNC91335.1"
                     /translation="MRVLVISDIHANYTALESVLKDAGQVDETWCLGDLVGYGPDPNA
                     VVEEIRDIPNLTCLLGNHDVAAIGKMPLETFNGDARRSLMYHEKVLSASNLDYMRSLS
                     SHTRVCGDATLAHGSPRDPLWEYILNSFSAQLNFDHFNTTSCFVGHSHIQCSFVMDEN
                     SRRVVLGQTKPDTTIQLRPKIILNPGSVGQPRDRDPRAAYAIYDTEAQTWTPRRVSYN
                     IPEVQERIRSAGLPEKHALRIADGW"
     gene            2067..3011
                     /locus_tag="PL000_20595"
     CDS             2067..3011
                     /locus_tag="PL000_20595"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNC91336.1"
                     /translation="MKKILIFSIIAAAMLITACGGGAAPEAPVMDVMPDSAGAPAAEQ
                     EFAPNAAFEESVANSGTGAAAAAVERIVIKNADIAIVVSDVTGRMEAIQTMAEEMGGF
                     VVASNLYQSYTNDNIEVPEATITVRVPAEKLNEALDLIKKDVVEVQSETVSGQDVTAE
                     YVDLQSNLRNLELAEKDLTRFLEEATTTEDVVNIFNQLTYYRQQIEVTKGQIKYYEEA
                     AALSSVSVRIVAEETIKPLTIGNWEPKGVALESIQDLVDFLQGFSEFLIRFLLYILPV
                     FLTIAAFIYLLFLGARAMFRKMRGDKPKKEQPQEVEKK"
     gene            3107..3817
                     /gene="rsmG"
                     /locus_tag="PL000_20600"
     CDS             3107..3817
                     /gene="rsmG"
                     /locus_tag="PL000_20600"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNC91337.1"
                     /translation="MENLAREALSLFNVHLTGRHILAFAAYERELLDWNQKFNLTAIR
                     DAESIRTKHFLDSLSCVQAWKATPPHRLVDVGTGAGFPGIPLKIIYPAMHLTLVESVG
                     KKVKFCQHIVDTLGLENVTVIHSRAEDIGQKAEHRESYDWAVARAVANLNVLSEYLLP
                     LVKLGGNVLAQKGESGPAEAQSAEKAMKLLGGKLKQLIPVNLPGVADDRYLVLVDKVA
                     ATPPKYPRNAGMAAKTPL"
     gene            complement(3849..4481)
                     /locus_tag="PL000_20605"
     CDS             complement(3849..4481)
                     /locus_tag="PL000_20605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91338.1"
                     /translation="MKILLCDDQAVIRDGLEMLLTLEKDFQVIGSAQDGAEAVELAAQ
                     KQPDLILMDLKMPMMNGIEATREIRARFPQIKILVLTTYDDDEWVFDAIRAGASGYLL
                     KDTPRQKIVEAIRGTMEGKSFVDPAIAGKLLNQVASKQTQPTSVLKEKLTERELDVLR
                     LIAKGINNGDIAAHLHLSEGTVRNHVSAILEKLGVSDRTQAAVIAIQHGL"
     gene            complement(4554..>4888)
                     /locus_tag="PL000_20610"
     CDS             complement(4554..>4888)
                     /locus_tag="PL000_20610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91339.1"
                     /translation="TRFKLDLDLALPNPMPSLSPDVEQTILRITQEAIENITHHSRAN
                     KFTIRLESNGHTTLTIRDNGVGFDMRSKEPTGHFGLIGMRERAELAGGKLKIESEKGK
                     GTKVVLTI"
BASE COUNT         1199 a         1262 c         1300 g         1127 t
ORIGIN      
        1 tggaagtata atgaccatat atgtccatcc tttccgataa acacatcctg cttggggtga
       61 cgggttccat tgcgggctat aaagccgcag atctggcatc caagcttgcg caatcaggcg
      121 caaaggtgga tgtgcttctg accgaagccg gggaaaagtt cgtcacacca ctcaccttcc
      181 aatctgtcac aggccgccgc gcatatacag ataaagacct atggggccac gaagcccatg
      241 tcttgcatgt cagctttgga aagacagccg acctgctggt cattgcccct tgcacggcag
      301 atacaattgc aaaactcgcg aacggcattg ccgataatct cttaactgtg accgcccttg
      361 cggcaacctg tccattggtg atcgcacctg ccatggatgg cggtatgttc aaccacccag
      421 ccacgcagga aaatcttgca aaattaaagg aacgtggcgc aatcatcatt gggcctgcag
      481 aaggtcacct tgcttcgggg ttggttggca caggccgaat ggttgagcct gttgaaattc
      541 tcgggcaaat tcggatggtg ctgggcaggc ggtatggaaa gctcgccggg aaaaaggttg
      601 ttgtcacggc aggcggaact caggaagcgc ttgacccggt gcgcgtcatc accaatcatt
      661 catcggggaa acagggatat gccatcgccc aggccgcgct cgattcaggc gcagacgttt
      721 gtctggtcac tgctccgacg gcattgaccg cccctgtggg agcgcgtgtt gtcaaagtaa
      781 acagtgcgga agaaatgctc caggctgtat tggccgagtc agcagatgcg ctggtgatgg
      841 ccgcagccgc ggcggatttc cgcccgaaac acattgcaaa agacaaaatg aaaaagcgcg
      901 acggtattcc gcagatcgag cttgaagcgg cgcccgatat ccttaaaaca gtatcaggca
      961 cacccgttga aaagaagcgg tttcgggtca tggtcggttt tgcggcggag agtcaaaacc
     1021 ttttggagaa cgcagatgaa aagctgaaat ccaaaaaact ggatttcatc gttgcgaacg
     1081 acatttctgc aaacgacgca gggtttgccg tggatacgaa ccgcgtcaca tttttgtttg
     1141 caaatggagc cagggaaaat ttgccgctga caagcaagat ggaagtggct gaaatcatca
     1201 tcgaacacat atcacggttg ctggagtgaa aatgcgagtt ttggtcattt ctgatattca
     1261 tgcaaattac accgcccttg aatctgtctt aaaagatgcg ggccaggtgg atgaaacctg
     1321 gtgtcttggc gaccttgttg gctacggccc agaccccaac gcggtggtgg aagagattcg
     1381 cgacatcccc aacctgacct gcctgttggg caaccatgac gtggccgcca ttggcaaaat
     1441 gccgcttgaa actttcaacg gggatgcgcg ccgatcgttg atgtatcacg aaaaggtgct
     1501 ttcagcatcc aacctggatt acatgcggtc gctctcttcg cacacgcggg tatgtggaga
     1561 tgccacgctc gcacacggca gcccgcgcga cccgttgtgg gaatacattt tgaattcgtt
     1621 ttcagcgcag cttaatttcg accatttcaa cacaacaagt tgtttcgtgg ggcattccca
     1681 catccaatgc tcgtttgtca tggatgaaaa ttccaggcgg gtcgttctcg gccagaccaa
     1741 acccgatacg acaattcagt tacgcccgaa gatcatcctc aacccgggtt cggtgggcca
     1801 gccgcgtgac cgcgacccgc gtgccgcgta cgctatttac gacaccgaag cccaaacatg
     1861 gactccccgc cgcgtgagtt acaacattcc cgaagtgcag gaacgcatcc gttcggctgg
     1921 tttgcctgaa aagcacgccc tgcgcattgc tgatggctgg tagtactaaa gttgggccag
     1981 agaaaatcgg gtgtcaggaa ctttctctcg atcatgttcg ttccttaggt agagaaatca
     2041 ccataaggta aaaaggagaa aagatcatga aaaagatttt gatattcagc attatcgccg
     2101 ccgccatgct gatcaccgcc tgtggaggtg gtgcagcccc tgaagccccc gtcatggatg
     2161 tgatgcctga ctcagcgggt gcgcccgcag cagagcagga atttgcgccc aatgccgcat
     2221 ttgaagaaag tgttgccaat agcggcacag gtgctgccgc cgccgcagtt gagcggatcg
     2281 tcatcaagaa cgccgacatt gccattgtgg tttccgatgt aacaggccgc atggaagcca
     2341 ttcagaccat ggctgaggaa atgggcggat ttgttgttgc ctcgaacctg taccaaagtt
     2401 acaccaatga caatatcgaa gtacccgaag cgacgatcac cgtccgtgtg cccgctgaaa
     2461 agttgaatga agcgctcgat ctcatcaaga aggatgtggt tgaggtgcag agcgaaacgg
     2521 tctccgggca ggatgtaacc gccgaatacg tggatttaca atcgaacctt cgaaatcttg
     2581 agcttgctga aaaggatctg accagattcc tcgaagaagc caccaccacc gaagatgtcg
     2641 tcaatatttt caaccaactc acctactacc gccagcagat cgaagtgacc aagggacaga
     2701 tcaaatacta cgaggaagcc gccgcacttt cttcggtcag tgttcgcatc gtcgctgagg
     2761 aaaccatcaa accgcttacc atcggcaatt gggaacccaa gggtgtggcg ctcgaatcaa
     2821 tacaagatct ggttgacttc ctccagggct tttctgaatt cctgatccgt ttcctgctct
     2881 atatcctgcc tgtcttcctt accattgccg ctttcattta cctcttgttc ctcggcgcac
     2941 gggccatgtt ccgcaagatg cgtggagata aaccaaagaa agaacaaccg caggaagtgg
     3001 aaaagaagta agaagtgttc agtcagtagg aaattgggga tagtgaatag ggaatagtaa
     3061 agagaggccg ctgaaaagtg gcctctcttt ttgctataat cacctcatgg aaaatctggc
     3121 ccgcgaagca ctctcgctct tcaatgttca cctgacggga aggcatattc tggcatttgc
     3181 cgcctacgaa cgtgagcttt tggattggaa tcaaaaattc aacctgaccg ccattcgaga
     3241 tgcggaatcc atccgtacca agcatttttt ggattcacta tcctgtgtgc aggcgtggaa
     3301 ggccactccc ccgcatcgcc tcgtggacgt gggaacgggt gccggtttcc cgggcattcc
     3361 cctgaaaatc atctatcctg ccatgcattt gactttggtt gaatcggtcg gaaagaaggt
     3421 gaaattctgc caacacattg tggatacgct tgggttggaa aatgtgaccg tgatccattc
     3481 acgggcggaa gatatcggcc aaaaagcgga acaccgcgaa agctatgact gggctgtggc
     3541 ccgtgctgtg gccaacctca acgttttgag cgaatacctg cttccactgg ttaagctggg
     3601 cggaaacgtg ctcgcccaaa aaggcgaaag cggacccgcc gaagcgcaat ccgctgaaaa
     3661 ggcgatgaaa ttattgggtg gaaaactgaa acaactgatc cctgtgaacc tgccgggggt
     3721 ggccgatgac cgctatctcg tgcttgtaga taaggttgcc gccacgccgc caaaatatcc
     3781 gcgcaatgca gggatggcag caaaaactcc gttgtgagtc atcaacaact gttcgccgaa
     3841 aaccgtcctc acaacccatg ctgaatggca atgactgccg cctgggttcg atcggaaact
     3901 cccaacttct ccaaaatcgc gctgacgtga ttccgcaccg tgccttcaga taaatgcagg
     3961 tgtgcggcga tatcaccgtt gttaattccc ttggcgatca aacgcaaaac atccaattca
     4021 cgctcggtga gcttttcttt caaaacagag gtgggctggg tttgtttgct ggcaacttga
     4081 tttaataact ttcccgcaat ggcagggtca acaaaagatt tgccttccat cgtgccgcga
     4141 atggcctcca cgatcttctg gcgcggtgta tctttgagca ggtaccctga agcgcctgca
     4201 cggatggcat cgaagaccca ttcatcgtca tcgtaggtcg tcagcacgag gatcttgatc
     4261 tggggaaaac gcgcgcgaat ttcccgcgtg gcttcaatgc cgttcatcat cggcattttc
     4321 aggtccatca aaatcaggtc gggttgtttt tgcgcggcaa gttccacggc ttctgcgccg
     4381 tcttgcgcag agccgatgac ctggaaatcc ttttcaaggg tgagaagcat ttccaatcca
     4441 tcgcggatca cagcctggtc gtcacagaga agtattttca tggttgaatt ctcggcaatg
     4501 gacgatggat cacagaccgt tttacatcag ccatcgttgc ctgcctgcaa ggattatatc
     4561 gtcagcacaa ccttcgtgcc ttttcccttt tcgctttcga tctttaattt accgccagca
     4621 agttcggcgc gttcacgcat accgatgagg ccgaaatgtc ccgtcggttc ttttgagcgc
     4681 atgtcaaaac caacgccgtt atcacggatg gtcaatgtgg tatgcccatt gctttcaagg
     4741 cggatggtga atttgttggc gcgtgaatga tgggtgatgt tttcaatggc ttcctgcgta
     4801 atgcgcagga tggtttgctc cacgtctggg ctgagagaag gcatggggtt cggcagagcc
     4861 aggtcaaggt ccagtttgaa gcgagtgg
//
LOCUS       DAOQLC010000222         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8098_length_4879_cov_14.487977, whole genome shotgun sequence.
ACCESSION   DAOQLC010000222 DAOQLC010000000
VERSION     DAOQLC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8098_length_4879_cov_14.487977"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..815)
                     /locus_tag="PL000_20615"
     CDS             complement(156..815)
                     /locus_tag="PL000_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91340.1"
                     /translation="MSEASPQRRSWIETLSSLTPLILGLLVTGVGATFTQIYNYRQLQ
                     LNQLAALDKFRPLLTSEKPEEREFAYASFAALGYDELALKMISLKNDGAGRAVVQDIS
                     ASGAGIAKVTATQILQSIPARVYLHIGEESLRPVGNAVVQALSQAGLQPMGIENISGK
                     AGVPNQTQVRFFNDADRPVAESVASLLTAQGVKGVVTVNNQGLKAKPGTIEVWFSKEI
                     Q"
     gene            complement(882..1721)
                     /locus_tag="PL000_20620"
     CDS             complement(882..1721)
                     /locus_tag="PL000_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91341.1"
                     /translation="MSEQERYEILKKEVDSLQAQLNRDQDPWYKKPSNLISAIALLFS
                     FGTTIVSYINSHAEDVRSNHREAMQIIQRITKLPYENFELLQKYKNSGQGQALSGMIN
                     QENILLATQATNLIKRYPDTFSATEFFSVATALATSNIVGEVPYLFETAIEKAETSND
                     YSVSTRSYAYFLYSKGDYTEGKKYYEMALDVWSKFPERNMYIVNSTDLLTLMYWSQAE
                     LMVNNIRGAEKNIDLAKEKLEQLPPTPMTQSLAMQINETIGFIEQAKTGAGQQMPHPD
                     LMQ"
     gene            complement(2186..2527)
                     /locus_tag="PL000_20625"
     CDS             complement(2186..2527)
                     /locus_tag="PL000_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91342.1"
                     /translation="MVYCSRFTCPINSQKQKTVVCPQFSETYDDHTLPQSQIAFVEGY
                     WHYRFIYDEELQIVSVDGKREAERSGWPYAYSISLPSGRHWIQLAVLRNSGEIARCAF
                     EETFESSIVTN"
     gene            3336..3698
                     /locus_tag="PL000_20630"
     CDS             3336..3698
                     /locus_tag="PL000_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91343.1"
                     /translation="MKRHIAIVSLFFPLACFGADMYRAPSAGDSGEYYILKQEAIGAG
                     MFKVLSNRVGKGAAYTDFTELKVNCTSQQYFELAGGEADGKKMMPPSELTPLKNPKWV
                     SLVPEGRGDRPRFSVFGY"
     gene            4072..4848
                     /gene="blaOXA"
                     /locus_tag="PL000_20635"
     CDS             4072..4848
                     /gene="blaOXA"
                     /locus_tag="PL000_20635"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HNC91344.1"
                     /translation="MNIRAFSLICLFLSVQASAEDQSIADLFTKQEVSGTIVISSLRG
                     GETFIHNDFRAAQRFSPASTFKILNTLISLEERAISRNGEVMMWDGKIHDLPDWNRDQ
                     TLESAFKVSCVWCFQQMAKEVGVEKYRAYLSKAEFGELSVPFEVTTFWLDGSLKVSAL
                     EQVALLKKIYRRTLPFSASSYEALSDVMLVEKTGSYALRAKTGWATRMTPQVGWYVGY
                     VEKSDDVWFFATNVDIRNQEDLPVRQKVTRDALQVKGIIP"
BASE COUNT         1111 a         1229 c         1342 g         1197 t
ORIGIN      
        1 gaactgtggg tcacgcgctg tctccggact ggctcatctg acgaaagtcc tctgcttttt
       61 tccctgcgac aactaccgat gcaaacgccg acagagccga ccagcagcgc cagcattcgg
      121 agtgcctgtg ctggccgcac caacgctgat caggctcatt ggatctcctt tgagaaccac
      181 acctcgatcg ttcccggttt ggctttcagg ccttggttgt tcaccgtgac gacacccttc
      241 actccctgtg ccgtgagcag tgaagccact gactcggcga caggcctgtc ggcgtcattg
      301 aagaaccgaa cttgtgtctg attcggaacc ccggcttttc cggagatatt ttcgatgccc
      361 atgggttgaa ggcctgcctg agacaatgct tgcacaactg cattgccaac aggccggaga
      421 gactcttcgc caatgtgcag gtacacccgc gcgggaatgg actggagaat ctgggtggca
      481 gtgactttgg cgattccggc cccagatgcc gagatatctt gcaccacggc gcggccggcg
      541 ccgtcgtttt tcagggagat cattttgagc gcaagctcat catagcccag tgccgcaaat
      601 gaagcgtagg cgaactcccg ttcctcgggc ttttcagagg tcaaaagcgg gcggaatttg
      661 tccagcgccg cgagttgatt gagctgaagc tgcctgtagt tatagatctg cgtgaatgtc
      721 gcgccaacac ctgtgaccag caggccaagg ataagcggtg tgaggcttga gagtgtctcg
      781 atccaggatc tgcgctgtgg cgatgcctcg gacatttttc attcctcatg caatgaattc
      841 tgatatcagt gtcaggaatg ggcgcaacga tgcaggcgat atcattgcat gaggtcagga
      901 tgtggcatct gctgtccggc accggttttc gcctgctcaa tgaagccgat ggtttcgttt
      961 atctgcatcg ccaatgattg tgtcatcgga gttggtggca gttgctcaag tttctctttt
     1021 gctagatcga tgtttttttc tgctcccctg atgttgttga ccatcagttc agcctggctc
     1081 caatacatca gcgtcagaag atccgtcgag ttcacgatgt acatgtttcg ttcggggaat
     1141 ttgctccaga catcaagcgc catttcatag tattttttac cttccgtgta gtcacccttg
     1201 ctgtacagaa agtatgcgta cgaccgtgtt gacacgctgt aatcattgga tgtttcagct
     1261 ttttcgattg cggtctcgaa aagatatggc acctctccaa cgatattcga ggttgcaaga
     1321 gctgtggcaa ccgaaaaaaa ttccgttgcg ctgaaagtgt ctggatatcg cttgatcaag
     1381 ttggttgcct gggtggcgag aagaatgttc tcctggttga tcatgccgga cagcgcctga
     1441 ccctgaccgc tgttcttgta tttttgcagg agctcgaaat tttcataggg aagtttggtg
     1501 attctctgga tgatctgcat ggcttcacga tggttcgatc tgacatcctc agcatgggag
     1561 tttatataag aaacgatcgt ggttccaaac gagaacagca gtgcaattgc tgaaatcagg
     1621 ttggatggtt ttttgtacca tggatcctga tcgcgattca attgtgcctg aagggagtcg
     1681 acctcttttt tcagaatctc ataacgctcc tgttcactca tggctgtcat ttccatcctg
     1741 attggctggt ttcgtatcaa gggcttcgag cgcggaatat atcagttttt catggtctgg
     1801 gagggccaag gcattcaagg cccacgactg aagacgatac tgcttcgaac gttggtgagt
     1861 ggcatgtcct cgcagcacac aggaccgaag aggcatgctg ggaggcctct cgacagagcg
     1921 tccagcactc gaggacggcg cacaacacgc catgcttatc gcataatgcc cagacatgct
     1981 ccaaacgcga agcccggatt catctggcag gagtattgtc gtgggcagtc cgagttctgg
     2041 cggcagattg ccttctttcc gcataccgcg gaaaccttca attctcgtgt tgagttggaa
     2101 ggtgttgtca cgaccattgc cagcgacgcc gagaaagacg atgacgacgg gtgcgcaagc
     2161 aaaagctggt catgatcgag gcgtttcaat ttgtaacgat gctgctctcg aaagtctcct
     2221 cgaaggcaca cctggcgatc tcgccactgt tgcgaagaac tgccagttga atccaatgtc
     2281 gtcccgatgg caaactgatc gaataagcgt aaggccagcc acttctctca gcctcgcgct
     2341 tgccgtcgac tgacacgatc tgcaactcct cgtcgtagat aaaccggtaa tgccagtatc
     2401 cttccacgaa agcgatttga ctttgtggta acgtgtgatc gtcgtaggtt tcagagaatt
     2461 ggggacagac cacggttttc tgtttttggc tattgatcgg gcaagtgaat cttgagcagt
     2521 aaaccatggt ctgtccccat ttctccattt ctcgccgaac gttattcttg gtgggaagaa
     2581 cgcgagtgca tctggacaag gtagaagggt tgggggattt tcttgagctg gaagtagtct
     2641 gggaattggg gacgacagac cacggtttcc cgttacggtt ttctgccaaa gcggagcaga
     2701 aaccaggaaa ccgtggtctg tccccaattc ctgcggacgc caacactggc catgccttcg
     2761 gcattttcat ggccagtgtt ggtaccctac gcgcttcgcg ctccggcgcc ggttaacctg
     2821 ggcgttgggc tttctagtat taggggaaag gtatgaagaa aattattctt ggcgcggtaa
     2881 tttgttcagc ggtagtcagc ggatgcggag aacctcgcaa cccacaggct ggcggacagg
     2941 aaaccacagc gtttgtcatg tgccagaatc cagtagagac gcagcttcgc gccccatcca
     3001 ctgctgaatt tccctatatc acagcacctg gagtttccgc cgtgcacctc ggtgacgggg
     3061 tgtatagggt gcatgggttc gttgatgcac agaacgggtt cggggccatg ctgcgtactt
     3121 catggacatg cgaaatagcc gagaatgctg accaaacgtg gtccctgaga aatctcgaga
     3181 tcgataaatg agcgggctat cgcacttgat gcagtggcag caatcctcca tacaagttgc
     3241 gtagtgaacc tcaccctaca ttcaagcggg actgcgcaaa tgcgtgcagc cccttacttt
     3301 cgacgttgaa cccagacaaa aaaggagaat catctatgaa aagacacata gcaatcgtct
     3361 ctctattttt tcccttagct tgctttggtg cagatatgta ccgtgcgcca tcagccggag
     3421 atagcggcga gtactacata cttaagcaag aggccatcgg tgctggaatg ttcaaagtgc
     3481 tgagcaaccg agttggcaag ggcgctgcgt atacggactt cacagagctc aaagtaaatt
     3541 gcacgtcaca gcaatacttt gagcttgctg gcggcgaagc agatggaaaa aaaatgatgc
     3601 caccgtcaga gcttacgcca cttaagaatc caaagtgggt atctctagtt cctgagggac
     3661 gtggggacag accacggttt tctgtttttg gctattgatc gggcaagtga atcttgagca
     3721 gtaaaccgtg gtctgtcccc atttctcctg gccaatcaag acggctggga cagatacgaa
     3781 gcggcaaaat ggctcaccat gcgtcgatgg cttgaagcca atcccgacga tgagttagcg
     3841 aaagaggttc gagccaaact gatctcggag cctggacgcc atgtggctta cacgcgcgag
     3901 tacctaggct ggggtgtgtt cgcgctcatg ccgcgatgag cattggcgtc ttggtcgccg
     3961 tctcgccaaa gccgactttt gcgctttggg ctatcggcct aacccatcat tccaccggac
     4021 ctgcgcgaaa agttgcgcag gccggtgaat tcaaacgtta tgcatcacta tatgaacatt
     4081 cgtgccttct ccctcatctg cctattcctg tcagttcagg cttcggccga ggatcagtca
     4141 atagctgacc tgttcaccaa gcaggaggtc agtggaacaa ttgtcatctc gtcactccga
     4201 ggcggcgaaa cgttcatcca caatgatttt cgagcggcgc aacggttttc accggcgtcg
     4261 acgttcaaga ttctaaacac ccttatatcg ctcgaagaga gagccatctc acgcaacggc
     4321 gaggttatga tgtgggacgg taaaattcac gatctcccag attggaaccg ggatcagacc
     4381 ttggagagcg cattcaaggt ttcctgtgtc tggtgcttcc agcagatggc gaaagaggtt
     4441 ggtgtggaaa agtacagggc ctacctgagc aaggcggagt ttggcgagtt gagcgtgcca
     4501 ttcgaggtga ccacattctg gctcgacggt tcgctaaagg tcagcgcttt ggagcaggtg
     4561 gctctgctca agaagatata ccggcgcacc ctcccattca gcgcctcgtc atatgaagcg
     4621 ctaagtgatg tcatgcttgt cgagaagacc ggaagctatg ccctccgggc caaaacaggc
     4681 tgggcgacaa gaatgacgcc gcaagttggc tggtatgtcg gctacgtcga aaaaagcgac
     4741 gatgtctggt ttttcgcaac gaacgtagat atccggaacc aggaggacct gcctgtgagg
     4801 cagaaggtta ctcgagatgc attgcaggtt aagggaataa tcccgtgatg cataaccatt
     4861 cgctgcaggc gcgacggcc
//
LOCUS       DAOQLC010000223         4819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8285_length_4819_cov_27.225231, whole genome shotgun sequence.
ACCESSION   DAOQLC010000223 DAOQLC010000000
VERSION     DAOQLC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_4819_cov_27.225231"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..1558
                     /locus_tag="PL000_20640"
     CDS             239..1558
                     /locus_tag="PL000_20640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC91345.1"
                     /translation="MPHGASKPGYKLSDEVFQNIFLQAGDGIFLIDAQSCMLEMNPRG
                     CEILGYSREELIGQPVLSFQPEDELPHIQEKLSQLAVDKLVMTESFFIRKDGVRIPVE
                     ITGKLLSNGQIIGMLRDISERKQTEQALINSEQRFRSLVEQSPDGIFMADENGLVVEW
                     NRGQEAISGLPRHEALEKPIWEIHWNLMPDELRTHGMFARLEENFKEILASGLGPGLN
                     LPREGVFQHSDGIYRNVEIMMYTYKTDLGYRIGGIVRDITERKQAERRLEYLAMHDVL
                     TDLPNRQLFQDRLEIALGRAGRKPYGKLAVMLLDLDNFKEVNDLHGHIYGDHLLKVAA
                     LRLQTCLRKSDTAARMGGDEFTLIIEEANDLETCSSIAQKVLETLSQPMELDGKVLKV
                     TASIGISFLSPENDNAATLLRKADIAMYQAKRTRNCYKFYEIDVRAY"
     gene            1692..2108
                     /locus_tag="PL000_20645"
     CDS             1692..2108
                     /locus_tag="PL000_20645"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNC91346.1"
                     /translation="MKRILLTMFVTAFLFSACASAPATSLPTEAPISSTLPALTDPSQ
                     PITVKAGDTFMIVMESNPSTGYHWEMIGPLDDAHLELVSREYTASEPVMPGSGGVEVW
                     TLKAVSAGETVLVLGNYPPAQGESYTTETRFSISIQ"
     gene            2251..2832
                     /locus_tag="PL000_20650"
     CDS             2251..2832
                     /locus_tag="PL000_20650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91347.1"
                     /translation="MSANALKRTLVFVPILFALACSTLTPKPALSASEIEKEEEAIYA
                     FFAGTSSAVILENTSAGIGNPTPQEMRRQLQDTFQNISGDTIESFVERNAEPGQLSAE
                     MELGVDYVLLSEKELAEISSQPDWGETLAERYPGSYGYLIFSRVGFNRTLDQAVIYVG
                     NVAGPLMGSGDIFLMEKVNGEWVIKEQFMIWIS"
     gene            complement(2938..>4819)
                     /locus_tag="PL000_20655"
     CDS             complement(2938..>4819)
                     /locus_tag="PL000_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91348.1"
                     /translation="GTSIGSLEYRTKKTFMHEFSALLHEMGAHEFARQYEWSTWKSQP
                     NVLKRLNTDLEPARGLTAVDFRAGLTLLPFLPMSPGDIKLILQGIKRGSLVQFDRGDV
                     NQLESFIKKNSADFSDLLPLLEELKTCELVYRNSIPDITHHHLNLIRDKALRATIVNS
                     TITGWSVKKIVDTDAEKRLHANKSFFLFFILLGFIPFLGKILRLAFGRQDYRDHYKKM
                     LSSFTYFQRAMFANRMEALIRWYRGERITETLAERISSSLLLYLYHIPLSILPKKIHR
                     LLSDRGFFMDTLYNTIIRPVRLYFNPVLREEWLREIVSESRDRQVLSKEDAEEILGQI
                     HDPYIHKYLQSLAVHIAMAPITHVISTGLAILYLLNHPEIPRAEAYATAAGILAFFQV
                     IPVSPGSFARGLYVLFLAIKERNLKDYNLALPLSFFKYVGYISFPIQMTYSYPTLARC
                     MAGFWATRMARVIPVFGEGGALLEYKTFNIFYNWPLTIRRKMNERAEWRKTQKTRSWH
                     IIPIVALISAASWLLQKLHISTTGMLPPFALVAPSLILFGLLGGILVNTGSGGSSFSR
                     RVLMAVGSGVGIGFLAALGLLILSPEVETGLIAWISTIIWYSFITATFSTTSAVITEF
                     KV"
BASE COUNT         1269 a         1063 c         1247 g         1240 t
ORIGIN      
        1 cttagtgcct tggtgacttt gtggtctgca acactttact tatgcaagag gtcaattgta
       61 ttaatcaggc tgacgctata aaaactttcg ctcaatgaag attttggctt tgatttcact
      121 gttatcaggt cataaatatc agttttgagg gtattttaaa tatcaagggt aaaatctaac
      181 gcaatcatcg tggcgttcac gtgatgaacc atgccttaaa tgtttcagga ggttggttat
      241 gccgcatggt gcgtctaagc cgggttataa actttcagac gaggtttttc aaaacatctt
      301 tctgcaggcg ggggatggta tttttctgat tgacgctcaa agttgtatgc ttgaaatgaa
      361 tccgcggggc tgcgagattt tgggctattc aagagaggaa cttattgggc agcccgtatt
      421 gagttttcaa cctgaagatg aactccccca tattcaagaa aagttatctc aactggcagt
      481 ggataagctg gtcatgacgg aaagtttctt tattcgaaaa gacggcgtcc gtattcctgt
      541 tgagatcacc ggtaaactgc tttccaacgg tcagatcatc gggatgttac gggatatcag
      601 cgaacgcaaa caaactgagc aagccctgat caacagtgag caaagatttc gcagcctcgt
      661 ggaacaatca cctgatggaa tctttatggc cgatgaaaat gggcttgtcg ttgaatggaa
      721 ccgcggccag gaagccatct ctggtctgcc acgacatgag gccctggaaa aaccgatctg
      781 ggaaattcac tggaacctga tgcctgatga attgcggacc cacggcatgt tcgccaggct
      841 ggaggaaaac tttaaggaga ttctcgcctc tggacttggc ccgggcctga acctgccacg
      901 agagggagtc tttcaacatt cagatgggat ctatcgcaat gttgagatca tgatgtacac
      961 ctataagacg gatctcggtt atcgcattgg ggggatcgta cgcgatatta ccgagcgaaa
     1021 gcaggcagag cggcggcttg aataccttgc catgcatgat gtattgaccg acctgccaaa
     1081 ccgccagctt tttcaagacc gtttggaaat tgcccttggc cgtgccgggc gcaagccgta
     1141 tggaaaactt gcggtgatgc tgcttgatct tgataacttc aaagaggtca atgacctgca
     1201 cgggcatatc tatggagatc atttgctgaa ggttgctgca ttgcggctgc aaacctgcct
     1261 gcgaaaatcg gatacagcgg cacgaatggg cggagatgaa ttcacactga tcattgaaga
     1321 agccaacgat cttgaaacct gcagttccat tgcacaaaag gtgctggaaa cactctctca
     1381 gcccatggaa ctcgatggca aggttttgaa ggtgactgcc agtatcggca tcagcttttt
     1441 atcgcctgaa aatgacaatg ccgccaccct tctccgtaag gcggatatcg ccatgtatca
     1501 ggcaaaaagg acgcggaact gttacaagtt ttacgaaatt gatgtccgcg cctactgaaa
     1561 taaacaggag aataccagaa gcaggtcact ttatttgccc tgaaaattaa gactttccgg
     1621 ttgggaacaa atcaattcaa tttggcgtcg aaatgccgtt gccatttgac caaaaaactc
     1681 aaggagttcc catgaaacgt attttactaa ctatgttcgt aacagcattt ctgttttcgg
     1741 cctgtgcatc tgcgcctgcc acctcgctgc ctacggaagc cccgatctcg tccaccctgc
     1801 ctgctcttac agacccatct caaccgatca ctgtgaaagc gggagacacc ttcatgatcg
     1861 taatggaatc caatccttcc actggttatc attgggaaat gatcgggcca ttggatgatg
     1921 cccatttgga gcttgtttca cgcgaatata ccgcttcaga acctgtgatg cccggttccg
     1981 gcggtgtgga agtgtggaca ctcaaggcag tttctgcagg agagacggtt cttgttttag
     2041 gaaattatcc gcctgcacag ggtgagtcat acacaactga gacccggttt tcgatctcaa
     2101 tccaatagat ttcaaaaagg acggatatct aaaagatatc cgtccttttt gaaactgaca
     2161 gggatgccca tgaccaatat cctacctgca gatggaacaa tcagatcgtg caggatgtct
     2221 ttgagaaaat tcaaaaagga gaattgaatc atgtctgcga atgctttgaa acgaacgtta
     2281 gtttttgtgc cgatcctgtt tgctttggcg tgctccactt tgacgcccaa accagccctc
     2341 agtgcgtctg agatcgaaaa agaagaggag gccatttatg ccttttttgc aggtaccagt
     2401 tccgcggtga ttcttgagaa cacatccgcc gggatcggca acccgacccc tcaggaaatg
     2461 aggcggcaac ttcaggatac tttccagaat atttcaggtg atacgatcga aagttttgtt
     2521 gaacgaaatg cagaacccgg tcagctttcg gcggagatgg agttgggggt ggattatgtt
     2581 ctgctgagtg aaaaagaatt ggccgagatc tccagccagc ctgattgggg tgaaaccctt
     2641 gcagagagat atcccggttc gtatgggtat ttgatctttt cccgggtggg cttcaatcgt
     2701 actcttgacc aagcggtcat ttatgtgggc aatgtggctg ggcctttaat gggcagcggc
     2761 gatatctttc tgatggaaaa ggtcaatggg gaatgggtga tcaaagaaca gttcatgatc
     2821 tggatttcat aacggtcaat aaaactcaaa gttgcgagaa gtcttttgat ttctcgcaac
     2881 tttgagtttt caaagaatct ataaaattta tttcgatcaa ctacctggca aggctctcta
     2941 cactttgaat tcggtgatga ccgcgctggt ggtgctgaat gtggccgtaa tgaaggagta
     3001 ccaaatgatc gtagatatcc atgcgatcag gccggtttct acttcagggc tgagaatcaa
     3061 tagtcccaag gcggccagaa acccgatgcc cactccactc ccaacggcca tcagcaccct
     3121 tcttgaaaaa gaggagccgc cactgcctgt atttaccaaa atcccgccga gtagcccaaa
     3181 caagatcagg ctgggagcaa caagcgcaaa aggcggaagc attcccgtgg tagagatgtg
     3241 gagtttttgc aacagccagc ttgcagcgga gattaacgcc acaatcggga tgatgtgcca
     3301 tgaacgggtc ttttgcgttt tacgccattc agcccgttca ttcattttgc gacgaatggt
     3361 aagcggccag ttatagaaaa tattgaaggt tttatattcc agcagggcac cgccttcgcc
     3421 aaaaactgga atgacccgtg ccatgcgggt ggcccagaac ccggccatac aacgagccag
     3481 tgtggggtag ctataggtca tttggatcgg gaaggaaata tacccaacat atttgaagaa
     3541 ggagaggggc agggcgaggt tatagtcttt gaggtttctt tctttgatgg ctaaaaagag
     3601 gacatataat ccgcgcgcaa aagagcccgg cgaaacagga atgacttgaa agaaggccag
     3661 aatcccggcg gcggtggcgt aggcttcagc tcgcggaatc tccggatgat tcaataaata
     3721 gagaatcgca agcccggtgg agatgacgtg tgtgatggga gccatggcaa tgtggactgc
     3781 aaggctttgc aaatatttgt gaatatacgg gtcgtgaatt tgccccaata tctcttcggc
     3841 atcttctttg gaaagtacct gcctgtctct gctttcgcta acaatttctc ttagccattc
     3901 ttcccgcagc acggggttga aatacaaccg cacaggccga atgatggtgt tgtataaagt
     3961 gtccataaag aatccacggt ctgagagcag gcggtgaatt tttttaggca aaatggaaag
     4021 ggggatatga taaagataaa gcagcaggga tgaagatatt ctttcagcca atgtttctgt
     4081 gatgcgttcc ccgcgatacc agcgaatcaa ggcctccatg cggttggcaa acatggcgcg
     4141 ttggaaatac gtaaagctgg acaacatttt tttataatga tcccgataat cctgacgtcc
     4201 gaaagcgagc cgaaggattt tccccaagaa cgggatgaag cccaacagta tgaagaacag
     4261 gaaaaaggac ttgtttgcat gcaggcgttt ttccgcgtct gtatcaacga tcttttttac
     4321 gctccagcct gtgatcgtgc tgttgacgat ggtggcgcgt agtgctttgt cccggatgag
     4381 gtttagatgg tggtgggtga tatcagggat ggagtttcga tagaccagtt cgcatgtctt
     4441 taattcttcc aacagcggaa gcaggtctga gaaatcggcg gaatttttct tgataaagga
     4501 ttcaagctgg tttacatcgc cgcggtcaaa ttgcaccaga ctgccgcgtt tgattccttg
     4561 caagatcaat ttgatgtcac ctggactcat gggcaggaag ggcaacagag tcaggcctgc
     4621 ccgaaaatcc actgctgtta atccgcgagc aggttcaagg tctgtattga ggcgtttgag
     4681 gacattgggc tggcttttcc aggtggacca ttcgtattgt ctggcgaatt cgtgggcacc
     4741 catctcatgc agcagtgcag agaattcatg catgaaagtt ttttttgtgc gatattccaa
     4801 agagccgatg gaggtgcct
//
LOCUS       DAOQLC010000224         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8417_length_4763_cov_16.218777, whole genome shotgun sequence.
ACCESSION   DAOQLC010000224 DAOQLC010000000
VERSION     DAOQLC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8417_length_4763_cov_16.218777"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1101)
                     /locus_tag="PL000_20660"
     CDS             complement(7..1101)
                     /locus_tag="PL000_20660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC91349.1"
                     /translation="MSKPIIHVFFLITVLLSACTAKGTASPDTPTPSPETEVETLTFY
                     SWPDYMDPQIISDFEQKYNVKVNYVTGSSASEIYDHGKAERGEYDIATISDFVLPGFS
                     RQGLLAPLNRENIPNIKNLDPFFANPQYDPNNRYCLPYQWGSTSIGYNYKITGREITS
                     WGDMFDPRFKGKVTFLDSNREAMGAILLYLGYSPNTSNKDEINQARDFLIKQGDQVFS
                     YKGYDGWQWLDGSDDKDIALAMGYSGAVQRTMIKDPNVRFTIPKEGAIIWVDNLCIPA
                     TSKHKELAEKFLNYLYEPEVGAQLSNFILYASPNLASLPFIDKAYRESQIMYPSAEVR
                     DRLFYLVDTGYDTTKLYDDAWAEILAAHGE"
     gene            complement(1142..2233)
                     /locus_tag="PL000_20665"
     CDS             complement(1142..2233)
                     /locus_tag="PL000_20665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC91350.1"
                     /translation="MKPHATLTTLAFFLLSACSAGSSSADPTSTPVIAIAADQFQVTA
                     QQEAPQTPSPEPFQFNLPTPGAEPISGWRPPLYPVPWAMSPYDHFYFVRPVAADQVNW
                     PIADYRYGGIFFAQNIIHTGIDIDAEKGAPIIAAGPGTVVWAGWGLFTETPTNDSDPY
                     GLAVAIRHDFGYQGQQLYTVYAHMDEVIAVVGQHVETGDLIGLVGATGAVTGPHVHFE
                     VRLSQNSFHFTLNPELWIAPPQGWGVLVGRVMDEKKDTMRHKEVLVISEETGKSYLVR
                     TYGSGGAANADPYYNENLVLGDLPAGIYKLIINYDDEKKPGQTWVEIFPGQVTYFTFE
                     GENGFDSTRPPAPTLEFLPALPATPTPVR"
     gene            complement(2319..4241)
                     /locus_tag="PL000_20670"
     CDS             complement(2319..4241)
                     /locus_tag="PL000_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91351.1"
                     /translation="MPQHIPTSRPTQSVPTHTRRNAWTSLLLTILILAACAQPAAPTQ
                     SPEATLPPLNITSTPNAIRYSGEDNLVFLSIEENGYAHLFVQSNPPQNLPLTRITTGT
                     WNDIAPALSPDRTQLAFASDRNGFWDLYILNLQSGSINQITNSPQYDSAPSWSPDSQW
                     LVFETYTNENLEVSVVSVNDRTQPILPITQHPASDHSPVWAPDGRHVAFISTRSGDSD
                     VWLANLDLTGEDRFQNLSNTPFASENYPVWNFDGSQLLWASISQTIGYSGLYIWNAND
                     PNRTAYWIGDGTWGAWNETAEKIVAVTNGANQQYLTSYDIKGTLLLAPTPLPGRARGL
                     TWGFANLSNPLPETFTQAAQITPPALWAPQITPETDIPNERWHVVQIADVQAPYPQMH
                     DLVDESFNSLRNRVIIETGWDVLASLESAFIPLTTSLDPGLEEDWLYTGRAFAINPLM
                     ADTGWMLTLREDIGAQTYWRVYIRTTIQDGSLGEPIHNAPWNLSARYELNPRTYEQGG
                     EYATVPSGYWIDITALSAAYGWERLPALPNWRTYYAGARFTEFALTNGLTWYNAMLEL
                     YPVEALVTPTRVLAPSLTPTRTATPTETPRPTRTPRPTSTPTLSPTPTVSPTPTLSPT
                     VTDTPTPTQTPPTVIP"
     gene            complement(4254..4625)
                     /locus_tag="PL000_20675"
     CDS             complement(4254..4625)
                     /locus_tag="PL000_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91352.1"
                     /translation="MDFFFPEDNLNRATPEETKITTLTAQPYPDARRIRVNLEITPFQ
                     KRPYIEITLTDADNNEIAATSIVEPLSWKLELTMHIRGQLNNPYTLNAKLYYPEGPTN
                     QPITYTFDVEPNPDDPQPAAT"
BASE COUNT          833 a          936 c         1790 g         1204 t
ORIGIN      
        1 ctggctttac tctccatgcg cggcgaggat ctccgcccag gcatcgtcat acagcttggt
       61 ggtgtcgtaa cctgtatcta ccaaatagaa taaacgatcg cgcacctcgg ctgaggggta
      121 catgatctgg ctttcccgat atgccttgtc aataaagggc aaagaagcca aattggggct
      181 ggcatataag atgaagttcg aaagttgtgc cccaacttca ggttcataga gatagttcaa
      241 aaacttttca gccaattcct tgtgttttga ggtggctggg atgcacaggt tatccaccca
      301 gataatggcg ccttcttttg gaatcgtaaa acgcacgttg gggtctttga tcatcgtacg
      361 ctgtactgcg ccactgtagc ccatggcgag tgcaatgtct ttgtcgtcac ttccgtccag
      421 ccattgccag ccatcgtagc ctttgtagga aaatacttga tcgccttgtt taattaaaaa
      481 gtcgcgggct tgattgatct catccttgtt ggaggtgttt gggctgtagc cgaggtagag
      541 caatatggct cccattgcct cgcgatttga atcgaggaag gtaacttttc ccttgaagcg
      601 cggatcgaac atgtctcccc agctggtgat ctccctgcct gtaattttgt agttgtatcc
      661 aatgctggtt gaaccccatt gatatggaag gcagtaacgg ttgttggggt catattgcgg
      721 gtttgcgaag aacgggtcca gatttttgat gttcgggata ttctctctgt tcaacggggc
      781 gagcaggccc tggcgggaga agcccggcaa aacaaagtcg cttattgtgg ctatgtcgta
      841 ttcgccgcgt tccgctttgc catggtcata tatctcggag gcggaagagc ctgtgacata
      901 gttgactttt acattgtatt tctgctcaaa atctgaaatg atctgcgggt ccatataatc
      961 tggccagctg tagaaggtga gagtctccac ttctgtctct gggctgggtg tcggcgtatc
     1021 gggcgaggct gtgccttttg ctgtgcaagc tgagagcaag actgtaatga gaaaaaagac
     1081 atgaatgatc ggtttggaca tttggctgtt cccttttgta cgttgatgat tttttacgca
     1141 actaccgaac cggcgtaggc gtggccggta gtgcgggtaa aaactcgagc gtgggtgcgg
     1201 gcggacgggt ggaatcaaat ccgttttcgc cttcgaaggt gaagtaggtt acctgaccgg
     1261 ggaagatctc cacccaggtt tgtccgggct ttttctcgtc atcataatta atgatgagtt
     1321 tgtaaatgcc cgccggtaga tcaccgagga caagattttc attgtagtat gggtcggcgt
     1381 tggcggctcc gccgctgccg taggtgcgca ccagatacga tttgcccgtt tcttcagaga
     1441 tgacaagcac ttctttgtgg cgcatcgtgt ctttcttttc atccatcacg cggccaacga
     1501 gcacgcccca gccctgcggg ggggcgatcc ataattcggg gttgagggtg aagtggaagg
     1561 aattttgtga gaggcgcact tcaaaatgga catgcggccc ggtcactgcg ccggtggcgc
     1621 cgaccaaccc gatcaggtcg cctgtttcca cgtgctggcc gaccacggcg atcacttcat
     1681 ccatgtgggc gtagacggtg tagagctgct gaccttgata gccaaaatcg tggcgaatgg
     1741 caacggccag accgtagggg tctgagtcgt tggtgggggt ttcggtgaag agaccccagc
     1801 ccgcccagac cacggtgccc ggcccggcgg cgatgatggg tgcgcctttt tcggcatcga
     1861 tatcaatgcc agtgtggatg atgttttgcg cgaagaaaat accgccgtag cgataatcgg
     1921 caatgggcca gttgacttga tcggcggcga cggggcggac gaagtagaag tggtcgtaag
     1981 gtgacatggc ccacggcaca gggtaaagcg gaggacgcca gcctgagatc ggttctgcgc
     2041 cgggggtggg gaggttgaat tgaaaaggtt ccggtgatgg ggtttgcggc gcttcctgct
     2101 gcgcagtgac ttgaaactgg tcggccgcaa tggctatgac cggcgtcgaa gttgggtctg
     2161 cactacttga gcctgcgcta caggctgata ataggaagaa ggcaagtgtg gtgagggtgg
     2221 cgtggggttt caaagttggt gattcttatg aaggtttgtt ttgcggtgat cgagacagta
     2281 aatgaaggat gcagtgaagc aacgagtcat tcatgcggtt agggaatgac cgttggcggg
     2341 gtttgcgttg gggttggggt gtcggtgact gtgggagaca gtgtgggggt cggtgagacg
     2401 gttggtgtgg gcgacaaggt gggtgtgctg gttgggcgcg gggtgcgtgt ggggcggggt
     2461 gtttcagtgg gtgttgccgt gcgtgtaggc gtgaggctgg gggcaaggac gcgggtgggt
     2521 gtgacaaggg cttcgacggg gtaaagctca agcatggcgt tgtaccaggt gaggccgttg
     2581 gtgagggcga attctgtgaa gcgggcgcct gcgtagtatg tgcgccagtt ggggagggcg
     2641 gggaggcgtt cccagccgta ggcggcgctg agtgcggtga tatctatcca gtagcctgag
     2701 gggacggtgg cgtattctcc gccttgttcg taggtgcggg ggttgagttc gtagcgggcg
     2761 ctgaggttcc acggggcgtt gtgaatgggt tcgccgaggg agccatcttg tatggtggtg
     2821 cggatgtaga cgcgccagta ggtttgggcg ccgatatctt cgcggagggt gagcatccag
     2881 cctgtatcgg ccatgagggg gttgatggcg aaggcgcggc cggtgtagag ccagtcttct
     2941 tcgaggccgg ggtcgaggct ggtggtgagg gggatgaagg cgctttcgag gctggcgagg
     3001 acgtcccagc ctgtttcgat gatgacacgg ttgcggaggc tgttgaagga ttcatccaca
     3061 aggtcgtgca tttgcgggta gggcgcctgt acgtcggcga tctggacgac gtgccagcgt
     3121 tcgttgggga tgtcggtttc gggggtgatc tgcggtgccc atagtgcggg cggggtgatc
     3181 tgtgcggcct gggtgaaggt ttcggggagc gggttggata ggttggcgaa gccccaggtg
     3241 agtccgcggg cgcggccggg gagtggggtg ggtgcgagga ggagggtgcc tttgatgtcg
     3301 taggaggtga ggtattgctg gttggcgccg ttggtgactg cgactatttt ttcggcggtt
     3361 tcgttccatg cgccccaggt gccgtcgccg atccagtagg cggtgcggtt ggggtcgttg
     3421 gcgttccaga tgtagaggcc gctgtagccg atggtttgtg agatggaggc ccagaggagt
     3481 tgtgagccgt cgaagttcca gacggggtag ttttctgagg cgaagggtgt gttgctgagg
     3541 ttttggaagc ggtcttcgcc ggtgaggtcg aggttggcga gccagacgtc gctatcgccg
     3601 ctgcgggtgg agatgaaggc gacgtggcgg ccatcgggtg cccagacggg ggagtgatct
     3661 gaggcggggt gttgggtgat ggggaggatg ggttgtgtgc ggtcgttgac ggatacgacg
     3721 gagacttcga ggttttcgtt ggtgtaggtt tcgaagacga gccattgtga atctggggac
     3781 caggaggggg ctgagtcgta ttggggtgag ttggtgatct ggttgatgct tccgctttgg
     3841 aggttgagga tgtagaggtc ccagaagccg ttgcggtctg aggcgaaggc gagttgggtg
     3901 cggtcggggc tgagggctgg ggcgatgtcg ttccaggtgc ctgtggtgat gcgggtgagg
     3961 gggaggtttt ggggcgggtt gctttgtacg aagaggtggg cgtagccgtt ctcttcgatg
     4021 gagaggaaga cgaggttatc ttcgccgctg tagcggatgg cgttgggtgt gctggtgatg
     4081 ttgaggggtg ggagggtggc ttcgggggat tgggtgggtg cggcgggttg ggcgcaggct
     4141 gcaaggatga ggatggtgag gaggagtgag gtccatgcgt tgcggcgggt gtgggtgggc
     4201 acgctttggg ttgggcgtga ggtgggtata tgttgaggca tggaggattt cagttaggtt
     4261 gcggcaggtt gggggtcgtc gggattcggt tctacatcga atgtgtaggt gatgggttgg
     4321 ttggtggggc cttcggggta gtagagtttg gcgttgaggg tgtaggggtt gttgagttgg
     4381 ccgcggatgt gcatggtgag ttcaagtttc catgagaggg gttcgacgat gctggttgcg
     4441 gcgatttcgt tgttgtctgc gtcggtgagg gtgatttcga tgtaggggcg tttttggaag
     4501 ggggtgattt cgaggttgac gcggatgcgg cgggcgtcgg ggtagggttg tgcggtgagg
     4561 gtggtgatct ttgtttcttc gggtgtggcg cggttgaggt tgtcttcggg aaagaaaaag
     4621 tccatttggg tattataacc agatttgtgt gggtggggtt ttgtgtgggg tgggagttgg
     4681 attacagttt tgttactcac cccctcccgg cctcccccaa atgccaaagt gcggaatttg
     4741 ggggaggagg ggtggtgggt gtg
//
LOCUS       DAOQLC010000225         4645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8766_length_4645_cov_19.244227, whole genome shotgun sequence.
ACCESSION   DAOQLC010000225 DAOQLC010000000
VERSION     DAOQLC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8766_length_4645_cov_19.244227"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..266)
                     /locus_tag="PL000_20680"
     CDS             complement(36..266)
                     /locus_tag="PL000_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91353.1"
                     /translation="MKGVSVANPAAWKKGQITSTVLGGLILAAVNVASAFGVEVPIDV
                     NGANAIGTAILVVVNCVLTVTTSTKMGFGEEK"
     gene            complement(331..3234)
                     /locus_tag="PL000_20685"
     CDS             complement(331..3234)
                     /locus_tag="PL000_20685"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNC91354.1"
                     /translation="MFTTSSICRTCSSSWTKNNSKSDMSKSRTQSDFESCSTCGIERV
                     ASTLNEHHQCPFCVEEGIPLPEPESKYPKITVIPSKNTGPREIKEAPVSKALAEAFAL
                     PYAPPSFDSNAAEADPTKELAMRALCRRHLLPFVQRFRPKYIAGWVHEDICRRLERFV
                     KAVERGESPRLLLLLPPRSGKSELGSRNFPPWVLGSHPDWEIIAASHTQSLTLSFSRY
                     VRDLVRDPAYQAIFPALKLDPNSQSIENWNTLSGGGYMAAGVGTGISGRGCFSCNTLL
                     RTLTRSDIIPIVNVKVGEMVYGYDHRTGLVKQVEVLAVSLTRTKRQLYAVEDIVCTDD
                     HYFYTPDERSYTAARALKTNMSLLRRKEGQSVENLSCMLLPESSDAFDWESVPTVWGT
                     EGWSRIRVQKLPHGASTSRTYSDTYLHALREAVHEEGPRARISDQEVRCSEHLLLKGV
                     LQHSSEAADEGEGSLRRVWGATAQSESEEILFARLLLEHEEDSVYENDIQPRILDAKE
                     ITDHAKRPRLCDVRTEDVSGGSAPHRSRCGAQQGRELGAVVLSVSQVVSHTRGVCAGD
                     LEAFIQDEDGELVVDIQTESGNFFVGLTNLLVHNCHILVVDDVVKDAEAADSVTIREN
                     TWEWYISTAYTRLAPGGGVLGILTWWHENDWAGRIQEVMASGEGDKFEVVKYPAINEE
                     GDEYILPDDTIQQFAPESDPPPPGSRMTRVKGTAIHPARYTTEMMLKIKNNLISAGLK
                     RVWNALYQQNPIPDDGIFFTKAMVRYFTHPPRRQEMFVYQAWDFAITETTESDYTVCV
                     TIGQDTSDNLHILNVLRYRSGDGNAIVDDVLDQYEAFKPDLIGFEDGQIWKSLTSQFT
                     KRCEERKLYPNHEQLQALTDKKVRAGPLKGRMQAGKVFVRSNQPWTQVFIDELMKFPN
                     AKHDDQVDAASWCVRLTLTKSAPKPPPPPKIKSWRDRLTDFVANGPAPGHMSA"
     gene            complement(3303..3848)
                     /locus_tag="PL000_20690"
     CDS             complement(3303..3848)
                     /locus_tag="PL000_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91355.1"
                     /translation="MACFGNNGERIKRGDKKLVAQKKVPREKSIILTSMQADFVDATL
                     AGKSPYEASKAAGYADPFTNQNQPFKSQAVQLALRTARDELSSAAQITRADIIDGFME
                     AINVARLAADPTAMIKGWSETAKVLGLYAPEVKRIEMNMNAQRLQSKFESMTDEDLIE
                     IIQGRAKLAIEGEAERVEISE"
     gene            complement(3862..4329)
                     /locus_tag="PL000_20695"
     CDS             complement(3862..4329)
                     /locus_tag="PL000_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91356.1"
                     /translation="MNMTLMEQELERDEGLRLSAYKDPAGKLTIGVGRNLDDNPLSPA
                     EELVIGHDGRTQPITREQALYLLGNDIKATTLCLDRFLPWWQNKDEVRKRVLINMCFN
                     MGIQRLLKFEETLHWVRLGHYNAAASRMAESKWATQVGQRAVRLENMMRTGEV"
BASE COUNT         1024 a         1250 c         1130 g         1241 t
ORIGIN      
        1 gatcattcca actgcaatcg agatcaagaa acagttcatt tctcctctcc aaaacccatc
       61 ttcgtactgg tcgtcactgt cagcacgcaa ttcacaacaa ccaggatcgc cgtaccgata
      121 gcattggccc cgttcacgtc aattggcacc tccacaccaa atgcactggc gacgttgact
      181 gctgcgagaa tcaacccgcc cagcacagtc gaagtaatct gacccttctt ccaagcagcg
      241 gggttggcaa ccgaaacacc tttcatgaaa aggtcagata tatgcatgtc agcagtctaa
      301 cagagagtgc aaaagcatgc tagagcttca ttacgccgac atgtgccccg gagctggtcc
      361 attggccaca aaatcggtca ggcggtcgcg ccaactcttg attttcggcg gtggtggcgg
      421 tttcggtgcc gatttagtca aagtgagcct cacacaccaa ctcgctgcat ccacttggtc
      481 gtcgtgcttg gcattcggaa acttcatcaa ctcgtcgata aacacctgcg tccatggctg
      541 attagatcga acgaagactt ttccagcctg catcctgccc ttcaacggcc ccgctcggac
      601 ctttttgtcc gtcagcgcct gcaactgttc atgattcggg tagagcttac gctcctcgca
      661 ccgcttggta aactggctgg tgagcgactt ccaaatctgc ccgtcctcaa atccgatcaa
      721 atcaggcttg aacgcctcat actgatcgag cacgtcgtcc acaatagcgt taccgtcccc
      781 gctgcggtac cgcaacacat ttagaatgtg caagttatct gaagtgtcct ggccaatcgt
      841 cacgcacacc gtgtagtcac tttctgttgt ctcagtgatg gcgaagtccc aggcttggta
      901 aacaaacatc tcctggcgac ggggcggatg ggtgaaatat ctgaccatcg ctttggtgaa
      961 gaagataccg tcatctggaa tcgggttttg ttggtacagg gcattccaca ccctcttcaa
     1021 ccctgctgag atcaagttgt tcttgatctt cagcatcatc tccgttgtgt aacgtgcagg
     1081 gtggattgcc gtacctttta cgcgtgtcat tcgcgaccct ggtgggggcg ggtcagactc
     1141 tggtgcaaac tgctggatcg tatcgtcggg aagaatatat tcgtcccctt cctcgttgat
     1201 agcaggatac ttgactactt cgaacttatc cccctcaccc gaagccatga cctcctgtat
     1261 ccgccccgcc caatcatttt catgccacca ggtcaaaatt cccagcacgc caccacctgg
     1321 ggcaagacgg gtataggctg tcgaaatgta ccactcccat gtgttttctc ggatggttac
     1381 ggagtccgct gcctctgcgt ccttgacaac gtcgtctacg acgaggatgt gacagttatg
     1441 gacgaggagg ttggtaagcc ctacgaagaa gttgccagac tccgtctgaa tatcaactac
     1501 aagttcgccg tcttcgtcct gaataaaggc ttcaaggtct cctgcacaga ctccgcgagt
     1561 gtgtgataca acttgtgaca ccgagagcac aacagcacca agttcccgac cttgttgtgc
     1621 tccgcatcgt gatctatgtg gtgcacttcc acccgatacg tcttctgttc gcacatcgca
     1681 caggcgcggt ctttttgcgt gatcagtaat ttctttagcg tcgagtattc ttggttgtat
     1741 gtcgttttcg tagaccgagt cttcttcgtg ttcaagtagc aggcgtgcga acagtatttc
     1801 ttctgattca ctttgcgcag tggcacccca cacccttcgc aatgaccctt caccttcgtc
     1861 ggcagcttcg gatgaatgtt gtaacactcc cttgagcaga aggtgctctg agcaccgtac
     1921 ttcctgatcg ctgattcgtg ctcgtggacc ttcctcgtga actgcttcgc gcagtgcgtg
     1981 caagtaagtg tcagagtagg tgcgtgaggt agaagctccg tgtggcaact tttgcacacg
     2041 gatgcgggac cagccttccg tcccccacac cgtgggcaca ctctcccagt cgaatgcgtc
     2101 tgaggattcg gggagtagca tgcacgacag gttttcgacg ctttgtcctt ctttcctccg
     2161 caagagggac atgttcgttt taagagcgcg cgctgctgta taacttcttt cgtcaggggt
     2221 gtagaagtag tggtcgtcgg tacagacgat gtcttccacg gcgtagagct gccgcttggt
     2281 gcgagtgagg ctaacggcga gaacttcaac ttgcttgacg agacccgtcc tgtgatcgta
     2341 cccatatacc atctctccaa ctttgacatt gacgatgggt attatatcag agcgggtgag
     2401 agtacgtaat agagtattgc aggaaaaaca cccgcggcct gagatccctg ttcctacccc
     2461 agcagccatg tacccgccgc ccgaaagcgt gttccagttc tcaattgact ggctgttcgg
     2521 gtcgagcttc aaagctggga agatcgcctg atatgcgggg tctcgtacga ggtctcgcac
     2581 gtagcgtgaa aatgagagag tgagagactg cgtatgactt gcggcgatga tttcccaatc
     2641 tgggtgactt ccgagcaccc acggtgggaa gttgcgcgat ccaagctcgg atttcccact
     2701 tctcggaggg agcaacagga ggagcctcgg actttctcca cgttcaacag ctttgacgaa
     2761 gcgctcgagc ctgcggcaaa tatcctcatg gacccaacct gcgatgtatt tcggacggaa
     2821 gcgctgaaca aacggtaaca agtgcctgcg gcacagtgcg cgcatagcaa gttcttttgt
     2881 tggatcagct tctgcagcgt tgctatcgaa ggagggtggt gcatatggta gcgcaaacgc
     2941 ttcagccagc gctttggaga caggtgcctc cttaatctct cttgggccgg tattcttcga
     3001 aggaataaca gttatctttg ggtatttcga ctccggttcc ggcaggggaa taccctcttc
     3061 cacacaaaac gggcactggt ggtgctcgtt cagcgtgctg gcaacgcgtt caataccaca
     3121 agtcgaacag gactcgaagt cagactgtgt ccgggatttg ctcatatcgc tctttgaatt
     3181 gttctttgtc catgacgagc tgcacgtgcg gcaaatcgat gaagttgtaa acaatcacct
     3241 ctttccaccc gtcgattggt cggtacgctg taaaaagcac ctctacctcg cggttgcaga
     3301 ccttactctg atatttcgac acgctcagct tctccctcaa tagctagctt tgctcgacct
     3361 tggatgattt caatcaaatc ctcgtcagtc atggattcga acttgctttg caaacgctgg
     3421 gcgttcatgt tcatttcgat tcgtttgact tctggtgcgt acagacctag caccttggct
     3481 gtttccgacc aacctttgat cattgccgtt gggtcggcag ccaatcgagc tacattgatt
     3541 gcttccatga aaccgtcaat gatatcggcg cgcgtgattt gtgccgcact ggacaactca
     3601 tctcgcgctg tacgcagtgc gagctgcaca gcttgcgact tgaaaggttg gttctgattt
     3661 gtaaatgggt cagcgtagcc cgctgcttta gaagcttcgt aaggactttt tcccgcgaga
     3721 gttgcgtcga caaaatccgc ctgcatggag gtcaaaataa tcgatttttc tcgtggaact
     3781 tttttttgtg ctaccaattt cttatcacct cttttaatcc gctcaccgtt attgccgaag
     3841 caagccacat tgctacttcc actacacctc tcctgttctc atcatgtttt caagacggac
     3901 ggctcgttga ccaacctggg tcgcccactt tgattctgcc atacgtgacg ccgccgcgtt
     3961 gtaatgaccc agtcgcaccc agtgcagcgt ttcttcgaat ttcaaaagtc gttggattcc
     4021 catgttgaaa cacatattga tcaacacgcg ttttctaacc tcatccttgt tttgccacca
     4081 cggaaggaac cggtccagac acagggttgt tgccttgata tcgtttccaa gcaaatacag
     4141 cgcttgttct ctcgtgattg gttgcgtacg accgtcgtgg ccaattacga gttcttctgc
     4201 ggggctgagt ggattgtcat ccaggtttct tcccacaccg atagtgagtt ttcccgcagg
     4261 gtctttatac gcactgagcc tcaacccttc gtcacgctcg agttcttgct ccataagtgt
     4321 catgttcatg gtttcttctc ccgtagtctg agagagtata agagagatga agtcgatgtg
     4381 caaattttga aattctaaaa attttcaaaa ttttagctct acgtggttgg tacaaatttt
     4441 tataaaaata agttctacgt ggttggtgca aatttctaaa aattttagtt ctacgtggtt
     4501 ggtgggttac ccatcccctc tctccccgag agacggggtg gcttcggatt cggtttccga
     4561 tttccgaata aggagtctct tttgttgtac caccaaccac gaaaggaaac atcatgaaca
     4621 cgatcaccat caagatgaac aatgc
//
LOCUS       DAOQLC010000226         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8794_length_4635_cov_27.860262, whole genome shotgun sequence.
ACCESSION   DAOQLC010000226 DAOQLC010000000
VERSION     DAOQLC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8794_length_4635_cov_27.860262"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /gene="leuS"
                     /locus_tag="PL000_20700"
     CDS             <1..1503
                     /gene="leuS"
                     /locus_tag="PL000_20700"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNC91357.1"
                     /translation="PIWIADYVLMSYGTGAIMAVPAHDERDFAFAKKYDLPIIPVIQP
                     EGQGEAGVLESAFVGAGVMINSGMLDGTKVNTEKGRKNPSIAKVIDWLEGQGIGKESV
                     NYRYRDWLISRQRYWGAPIPMVNCEIHGWNPVPDEQLPVELPEDVEWKPTGESPLKLH
                     PTWKQTTCPVCNGPATRETDTMDTFMCSSWYHLRYLSPTYDQGPFDPAEYDYWMPVDT
                     YTGGIEHATMHLLYTRFFHKALRDIGIVKGNEPMTQLRNQGMVLGEDNEKMSKSRGNV
                     IAPDVLVERYGADTVRAYIMFFARWEMGAPWDSQGIEGAVRWVKRVWTLFTDPTPPTT
                     ASEETKKNLRRKVHQTLRRVTRNFEQFEFNTIVSSLMELMNEMYKAREAGAVGTEEWK
                     EAQDIYLRMMAPVTPHVAEELWSQLGKPYSVHTQQWPAVDEAAAKEDSIELPVQINGK
                     VRDRIIVAAEASEEEVKAAALASEVVQKYLEGKEPRKVIVAKGKLVSVVL"
     gene            complement(1624..1980)
                     /locus_tag="PL000_20705"
     CDS             complement(1624..1980)
                     /locus_tag="PL000_20705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC91358.1"
                     /translation="MDKTFIKDLLVRGIIGIRDWERVTPQDILINVTVFSDTRLAAQT
                     DDIAHCVDYSALAKKVQAHAETSARLTVEALANDLAEICLKENGAQKVIVRVEKPSAV
                     RFAKSVGVEIERSRNE"
     gene            complement(1986..2441)
                     /locus_tag="PL000_20710"
     CDS             complement(1986..2441)
                     /locus_tag="PL000_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91359.1"
                     /translation="MEDRELYGKSAIFGMGLGALLGLILGGATGALSLTWGGIQLAGL
                     VGLAAGAVTGALTGLISARVAGRTGGVSFGAYGGMAFGGFLGLILGAFIPASFRESIA
                     TDQMPYLTVLANGTFEMAILVSFLLACLGTAVGAWVAGRNFIPRDQREG"
     gene            2606..>4635
                     /locus_tag="PL000_20715"
     CDS             2606..>4635
                     /locus_tag="PL000_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91360.1"
                     /translation="MAEPLYFQWQNTVLCKTIYPMREPKLRDFLVYYMEIDLWKQYKD
                     KDISTLKTEVDAYITAQEFALVAAYKSYSALRSYFMLKDVRGYYLKYKPINEAELTEI
                     NNFHAIFIEGWPKDIRGERSFVEGQLQYWVNHRNMQKEAAKSKRRRVENMDPAHPKRP
                     IEAQELETMEGITLPMAEQELDQVRAFLKTYDKVEQRKLAWYKQSKADPAFSTSESEF
                     LVHYKPEAPITVQDIARWKVEEYKTSLEKKNQYELLEEIYQRFKKEPNRYPHWLQYMV
                     VHFSGMRYASAHNSWADPKLLLMRLRGSNAGSEIRKLDDAAVEKLCTEKIAVYETGTG
                     SNIPKLARASEKEWKDKVALHMLSVKASGPKTRRNGLTALQAEELNYEINSLSTEEAL
                     KALEDMKSTFPAWAWKEIVKLTPLRVNHVTDPNWEKLTPEEEAARYSQTYNDIRNVLD
                     AWENYDSSAWREEHGRSHELIVSRAVCNETAEHCQHLRGHLPPGGLTPKPKWYFNNET
                     EGKLPGDPRPFYTRPTSAADYVPGASIFWLRFVDKEPNAWQVAKEVKTKSGVGLLPDM
                     GKKKGDKSDPKATSTPWEYQKGETVTRSRTLINPETKVKSQQHQWLRWIHEATVAEVA
                     ETADGTMVITYETALPDDDKGTSSVGTFRMPLSWHLDDGPEDTYNRSFVGFVP"
BASE COUNT         1142 a         1187 c         1273 g         1033 t
ORIGIN      
        1 ccgatctgga tcgcggatta tgttttgatg tcttacggca cgggcgcgat catggctgtc
       61 cctgcgcatg atgagcgtga ctttgccttt gccaaaaagt atgacctgcc cattattcca
      121 gtgatccagc ctgagggtca gggcgaagcg ggtgtgttag agtccgcttt tgttggggct
      181 ggggtcatga tcaattcggg catgttggac ggcacgaaag tcaacaccga aaagggacgc
      241 aagaatccca gcattgcgaa agtgatcgat tggctggaag ggcagggcat tggaaaagaa
      301 tcggtcaact atcgttatcg cgattggttg atcagccgcc agcgttattg gggcgcgccg
      361 atcccgatgg tcaattgcga aattcatgga tggaatcccg tgcctgatga gcagttgccc
      421 gttgaactgc ccgaagatgt tgagtggaag ccgacgggcg aatctccgtt gaagttgcat
      481 cccacctgga agcaaaccac ctgccccgtg tgcaatggcc ccgctacgcg tgagaccgat
      541 acgatggata cgttcatgtg ttcgtcgtgg tatcacttgc gttacctgtc gccgacctac
      601 gatcagggtc cgttcgatcc tgctgaatat gattactgga tgcccgtgga tacgtacaca
      661 ggcggcattg aacatgccac catgcacctg ttgtatacgc gtttcttcca caaagccctg
      721 cgcgatattg gcattgtcaa aggcaatgag ccgatgacgc agttacgcaa tcagggcatg
      781 gtgttgggcg aagataacga gaagatgtcg aagagccgcg gcaacgtgat cgcgcccgat
      841 gtgttggttg aacgttatgg cgccgacacg gttcgtgcct acatcatgtt ctttgcgcgc
      901 tgggaaatgg gcgcaccctg ggactcgcaa ggtatcgagg gtgcggtccg ttgggtaaag
      961 cgtgtgtgga ctttgttcac cgacccaact ccgccgacga ctgcctctga agaaacgaaa
     1021 aagaatctgc gccgaaaggt tcaccaaacc ctgcgccgtg tgacacgtaa ctttgagcag
     1081 tttgaattca acacgatcgt gtcatcgttg atggagttga tgaacgaaat gtacaaggcg
     1141 cgcgaagcag gcgcggttgg cacggaagag tggaaggaag cccaggatat ttacttgaga
     1201 atgatggctc ccgtcacccc gcatgttgcc gaagaattgt ggtcgcagtt gggcaagccg
     1261 tattctgtcc atacccagca atggcccgcc gtggatgagg ctgctgccaa ggaagattcc
     1321 atcgaattgc ccgttcagat caacgggaaa gtgagagacc gcatcatcgt tgccgcagaa
     1381 gccagcgagg aagaggtcaa agcggctgct ttggcgagcg aggtggttca gaaatatctg
     1441 gaaggcaaag agcctcgcaa ggtgatcgtg gcaaaaggta aattggtcag cgtggttttg
     1501 tagttgactg gtttgctgaa agagtaaatt acccaggcca tcgcccgggg ttggattaaa
     1561 acagaatgcc tccgagagtc actcgactct cgggggcatt ctgcttttta ggttctcccg
     1621 tttttattcg ttgcggctgc gttcgatctc cacaccaacc gatttggcga accgcactgc
     1681 cgagggcttt tccacgcgaa cgatcacttt ttgagcgccg ttctctttca ggcaaatttc
     1741 ggcgaggtcg ttggccagtg cctccaccgt aaggcgtgcg gaagtttcag catgcgcctg
     1801 aacctttttt gccaatgcgc tgtaatccac acagtgggca atgtcatcgg tttgcgcggc
     1861 aaggcgtgtg tcgctgaata ccgtcacatt gatcaggata tcctgcggtg tcacccgctc
     1921 ccaatcacgg atgccgatga tgccgcgcac caacaggtct ttgataaaag ttttatccat
     1981 gcctgtcatc cttcgcgctg gtcacgtgga atgaaattac gtcccgccac ccatgcgcca
     2041 acagcggttc caaggcaggc cagcaaaaaa ctgaccagga tcgccatttc aaatgtccca
     2101 tttgcgagga cggtgaggta gggcatttgg tcggtggcga tgctttcgcg gaacgaagca
     2161 gggatgaagg ctcccaagat caggccaagg aatccgccaa atgccatgcc gccgtaagcc
     2221 ccaaagctga caccccccgt gcggcctgca acccgtgccg agatcagtcc agtcagtgcc
     2281 ccggtcactg ccccggctgc cagcccaaca agcccagcca gttgaattcc accccatgtc
     2341 aggctgaggg cgcctgtggc tccgcccagt atcaacccta gaagggctcc aagccccatg
     2401 ccaaagatcg cgctctttcc atataactct ctatcttcca tcgttaccgt ctcttttctg
     2461 tgggggtgtg cgtttgaatg caggcaagta tattttcatt gactctctcc tgcaagagca
     2521 ggaaatccgt ttcgtttttt gggcaggttt ggaataaaat gaggcatcat tttctcaaaa
     2581 cgtccaattc tcaaggaggc cactcatggc tgagccgctg tacttccaat ggcagaatac
     2641 tgttttgtgc aagaccattt acccaatgcg cgaaccgaaa ttgcgcgatt ttctggtcta
     2701 ttacatggaa attgatttgt ggaaacaata caaggacaag gacatttcca cactcaaaac
     2761 ggaagtggat gcgtacatca cggcacaaga gtttgccctg gttgccgcct acaaatctta
     2821 ctctgctttg cgttcctatt ttatgttgaa agatgtgcgg ggatattacc tgaagtacaa
     2881 gcctattaac gaagccgaat tgaccgagat caataatttt catgccattt ttatcgaagg
     2941 ctggcccaag gatatccgtg gtgagcggag ctttgtggag ggccaacttc aatattgggt
     3001 caatcatcgc aacatgcaga aagaggcggc caaatccaag cggcggcgcg ttgaaaatat
     3061 ggaccccgcc cacccgaagc gccccatcga agcgcaggaa cttgaaacga tggaaggcat
     3121 caccctgccc atggccgagc aggaactgga tcaggtgcgt gcttttctca agacctacga
     3181 taaggtcgag caaagaaaac tcgcgtggta caaacagtcg aaggctgatc cagctttcag
     3241 tacgtcagaa tcggagtttt tagtccacta taagcctgaa gcgccgatca cggttcagga
     3301 tattgcccgt tggaaggtgg aagaatacaa gacctccctc gaaaagaaga atcaatatga
     3361 actgctggag gaaatttatc agcgcttcaa gaaggaaccg aaccgttacc cgcattggct
     3421 gcaatacatg gtcgtccatt tttcggggat gcgttacgcg agtgcgcata attcatgggc
     3481 agaccccaag ttgctgctga tgcgtttgcg tggatcgaac gccgggagcg agattcgcaa
     3541 actggatgat gccgccgtgg agaaactctg caccgaaaag atcgcggtct acgaaactgg
     3601 aacagggtcc aacatcccca agctggctcg tgcctcagaa aaagaatgga aagataaagt
     3661 ggccctgcac atgctcagcg tgaaagccag cggacccaag acccgccgca acggtttgac
     3721 cgctttgcag gccgaagagt tgaattacga aataaattca ttgagtacag aggaagcgct
     3781 caaggcgctc gaagatatga aatccacctt ccctgcctgg gcgtggaagg aaatcgtcaa
     3841 gctcacgccg ctgcgtgtga accatgttac agatccgaat tgggagaaac taacccctga
     3901 agaggaagct gcgcgttatt cacagacata caacgatatt cgcaatgtgt tggatgcctg
     3961 ggaaaattat gattcatctg catggcgtga agagcatgga cgctctcatg agttaattgt
     4021 cagccgtgcg gtctgcaacg aaacggccga gcattgccag cacctgcgcg gacatttgcc
     4081 ccccggcggc ctgaccccca agccgaaatg gtatttcaat aatgagaccg agggcaaatt
     4141 gcccggtgat ccccgtccgt tctataccag gcccacgtct gcagcagatt atgtgcccgg
     4201 tgccagtatt ttctggctgc gctttgtaga taaagagcca aatgcctggc aggtggccaa
     4261 ggaagtgaaa accaaatcgg gtgtggggtt gctgccagac atgggaaaga aaaaaggcga
     4321 caaatccgat cccaaggcta cttctacgcc gtgggaatat caaaagggtg agacggtgac
     4381 ccgctcgcgg acattgatca accctgagac caaggtcaaa tctcaacagc accaatggct
     4441 gcgctggatc cacgaagcga cggtggctga ggtggctgaa accgccgatg ggacaatggt
     4501 catcacctat gaaaccgccc tgcccgatga tgataaaggt acgtcttccg ttggcacttt
     4561 tagaatgccg ctctcctggc accttgatga tggcccagaa gacacctata accgttcctt
     4621 cgtcggcttt gtgcc
//
LOCUS       DAOQLC010000227         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8903_length_4600_cov_20.448405, whole genome shotgun sequence.
ACCESSION   DAOQLC010000227 DAOQLC010000000
VERSION     DAOQLC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_4600_cov_20.448405"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..2831
                     /locus_tag="PL000_20720"
     CDS             252..2831
                     /locus_tag="PL000_20720"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91361.1"
                     /translation="MSAASISYPGAVQDFLQSLGLWRKLTSPARELLCSISNKLEPFG
                     VPSRLQDDSNDIRGFECLSLGPNGESYSTHAGSLIKEKIPEKTAHLTLFRISLLTAQS
                     LVDATTTNTPLSFWRSAYFNINASASLLDVKEFSQLLDNINPTFKILLEFKESLSHDQ
                     IKKLRNILDRHESRIELSLSYAEQLENDTRTLLNERIQSIRTGSKHLQHLYRDRDKEP
                     GYVINSLKELQNPNYVRNSQAKQNHLAESARNRQAVVIAEGIEDQDIKRFLQDRWDVS
                     KNGELWFQGHHITVPLPWRCALSPISSEDERQPRGYKLIADPRGTSKMRTSRGPIISI
                     HTGKRFALVGTIVAASALPFILVTSGIDGTAAKAASYLYLESTKEGEESIEEWRARYT
                     TLANTYKGINPETRKALSKLDLENAISIQERIIRNTPLGEQHGSQLGNEHFVLAQLYL
                     LKNSTREAELHLYKAYIANPSNPDIGLSLAEHYQENEEFSKAVDLYENRLIPDYAHLA
                     NQTTQPNLYKLALSSAHNDLGELYRVMGQLDKSEKHLASALTIRLKIDALNYSDSYLY
                     NNKSIDSLQAETLNNLGSLAADTKDINEAIFFYERAIQKKRSELEKHNTIANKSSFAR
                     TAHNYGTVLFSYGMYSQAENSFNEAITHRSQLVTLDPKNRSTQEGLANSLFALGSVYQ
                     KEGSITKAISKLDESAEHFKTLAAFSNNSYNLDLARVLDLKANILLNASQLEEALAAA
                     RIAAKTLDETEHDQTPSTFILEYLTCHTVLGAIFVARGDIIDAQESFSKANARINSLD
                     KTFADTLNDQEKSKFFQICTTVKRNLTHTEPSKEQTSCDTSSSIEKSTHTNLHGIKKA
                     NGS"
     gene            2756..3292
                     /locus_tag="PL000_20725"
     CDS             2756..3292
                     /locus_tag="PL000_20725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91362.1"
                     /translation="MRHLIINRKKYPHKPPRHQKGERIIKVLVIALSFAISTHALADE
                     PLSYRKASECISASKLDSLGSQQSAAAIQIHSAISSGAIIRSDLCDSLKNVIAIIAQT
                     GSSSGRQLEKDRKVSPALALHEYQLSIAQPDISRDIATATRNNSNQNFKTLMEAVALH
                     DNGKYEARDHILAQLEKR"
     gene            3289..4410
                     /locus_tag="PL000_20730"
     CDS             3289..4410
                     /locus_tag="PL000_20730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC91363.1"
                     /translation="MKNSKYIRFTALAALSMSGCLSNPSNPTAATALSPASSVPIPET
                     LIQIGEKKWSPSAQEFDPKCEHLIAPFDLTDNIPKLIELTTSITLSETVSANDNTANG
                     SKGGRHGLKHNIGLATRRAAARLNWLPMELEVLYGEYILDKRKDSLISENSKAGRALY
                     PKAEALIDSALGPIKEPTPYKFKVHVSSSAGENAEALPGGFIVIDKALLERHELKGKA
                     QFAVSHEIAHVLQRHQTRAMQAQIIDTVSLTSNVTELYKVIKDSKEKPKEVLKYILAG
                     KLIFQKHFEDQELQADACAARILDTTFKNRTNVVSSIRSFVSRLPPPEPEVTKDDEAT
                     ALFKVVTRPIDQHPTTNARIANLNFMVKEIYGGKAKSKS"
BASE COUNT         1441 a         1282 c          918 g          959 t
ORIGIN      
        1 ctggacgtcc ctgagcatgg cggaactgga tcggctgttc ctgcggggac ggtgacggag
       61 cagcccgaaa ttaggcaagt cagttgcgaa tcgatttgca tcaccaaaac tgattagtct
      121 gaagaccttg gctgcgacca agatactcaa gcaggaacac accgccgacc agaaaaatcc
      181 aacatcgata ctttcgcctc tacataaaat ctgccatgat tatgccgcgt ggtagttcat
      241 agaggcttgg catgtctgcg gcatccatct cctatccggg agcagttcag gactttctcc
      301 agagcttggg actctggcgg aagctcactt ctcccgctcg ggaattgctc tgttcaatca
      361 gcaacaaact agaaccgttc ggggttcctt cacgcctcca ggatgacagc aatgacatcc
      421 gcggattcga gtgtctttcg ctcggtccaa acggtgagag ttacagcacg cacgcaggaa
      481 gccttataaa ggaaaaaatc cctgaaaaaa cagcccatct cacattattc agaatctcgc
      541 tccttactgc ccagtcattg gttgacgcaa cgacaacaaa tacaccactc tctttctggc
      601 gcagcgccta cttcaatatt aatgccagcg catccctgct agacgtcaaa gaattttctc
      661 aactcctaga caacatcaat cccaccttca agattctcct agaattcaag gaaagcctca
      721 gccacgatca aatcaaaaag cttcgcaaca ttctcgaccg ccacgaatca cgcatagaac
      781 tgtcactaag ttacgcagaa caactcgaaa atgacacccg aactttattg aacgagagaa
      841 ttcaatctat tcggacggga tcaaagcacc ttcaacatct atatcgcgat cgtgacaaag
      901 aacccggata tgtcattaac agcctgaaag aactccaaaa ccccaactat gtccgcaatt
      961 cacaggcaaa gcaaaatcac ctagccgaga gcgctcgaaa tcggcaggcc gttgtgatcg
     1021 ctgaagggat tgaagatcag gacatcaaga gatttcttca agaccgttgg gacgtcagca
     1081 agaacggaga gctttggttt caggggcacc acattaccgt cccgctgccc tggagatgcg
     1141 cactatcccc aatcagcagt gaagacgaac ggcagccaag aggatataaa ctgattgccg
     1201 acccgagagg cacctccaaa atgaggacgt ctcgcggacc tatcataagc atccataccg
     1261 gcaaaaggtt tgcgctcgtc ggcaccattg tcgccgccag cgcattacca ttcatattgg
     1321 tcacttccgg gattgatgga accgcagcca aggcggcaag ttacctatat ctcgaatcca
     1381 cgaaagaggg tgaagagtcg atagaggagt ggcgcgcgcg ctacacgacg ctagcaaaca
     1441 cctacaaagg aatcaatcct gagacaagaa aagccttgtc gaagcttgat cttgaaaacg
     1501 ccatctcaat tcaagaacgg atcatcagga acaccccctt gggcgagcag catggcagcc
     1561 aactcggaaa cgagcatttc gtccttgctc aactatatct actaaagaat agcaccaggg
     1621 aagcggaact tcatttatac aaagcgtaca tcgccaatcc ctcaaatccg gatattggac
     1681 taagccttgc cgagcactac caggaaaacg aagaatttag caaagctgtc gatctgtatg
     1741 aaaaccgtct catcccagac tacgcccact tagcaaacca aacaacacaa ccaaatttat
     1801 acaaactcgc tttatcatcc gcccacaacg atctcggcga gctttaccgg gtcatgggcc
     1861 agcttgacaa atccgagaaa catctggcga gcgcattgac catcaggctc aaaatagatg
     1921 cgctaaatta cagcgattca tatctataca acaacaaatc tatcgattcg cttcaagcag
     1981 aaacactaaa caatctaggc tctctcgcgg cagacacaaa agacattaat gaagcaatat
     2041 tcttctatga acgcgcaatt caaaagaagc ggtcagaact cgaaaagcac aataccatag
     2101 ccaacaaatc ctcatttgca cgaacagctc acaattacgg caccgtacta ttttcctatg
     2161 gaatgtattc tcaagcagaa aacagcttta atgaagcaat tacccatcga agccaactag
     2221 tcactctcga cccaaaaaac cggtcgaccc aggaaggact agcaaactcc ctcttcgctc
     2281 ttggatctgt atatcaaaaa gagggcagca ttacaaaagc catatccaaa cttgacgaat
     2341 cagcagagca cttcaagacg ctagctgcat tctcaaacaa ctcttacaac ctagatctcg
     2401 cgcgagtact tgatctaaag gcgaacattc tattaaacgc aagccaactc gaggaggcgc
     2461 tggctgcagc gagaatagct gctaaaactc tcgacgaaac tgagcacgac cagactccca
     2521 gcacatttat ccttgaatac cttacatgcc acaccgttct cggcgcaata tttgtagcac
     2581 gtggcgacat cattgacgcg caggagagct tttcaaaggc aaacgcaaga attaatagcc
     2641 tcgacaagac attcgcggac accctaaacg atcaagaaaa atcgaaattc tttcaaatat
     2701 gcacgacagt aaaaagaaat ctcacccata ctgaaccatc aaaggaacaa acgtcatgcg
     2761 acacctcatc atcaatagaa aaaagtaccc acacaaacct ccacggcatc aaaaaggcga
     2821 acggatcata aaggtcttgg taattgcgct ctcatttgcg ataagcactc atgctttagc
     2881 agacgaacca ctctcttacc gaaaagccag tgagtgcatc agtgcatcaa agcttgactc
     2941 gcttggctcg cagcaaagcg ctgccgcaat acagatacat tcagcaatct cgtctggtgc
     3001 cataatccga tcagatctct gtgactcatt aaaaaatgtt attgccataa tagctcaaac
     3061 cggctcatca tctggccgtc aactagaaaa agacagaaaa gtcagccctg cacttgcgct
     3121 acacgaatat caattatcca ttgctcagcc agacatctcg cgagacattg ccaccgcaac
     3181 aaggaacaat tcaaatcaga acttcaagac attgatggag gccgtcgcac tgcacgacaa
     3241 tggaaagtat gaagcccggg atcacatcct tgcgcaactt gaaaaacgat gaaaaactct
     3301 aaatatattc gttttacggc actcgctgca ctctcgatgt ctggctgcct ctccaacccg
     3361 tcaaatccaa ctgcagccac tgcactttcg ccagcaagct cagttccaat accagaaact
     3421 ttgatacaaa ttggggaaaa aaagtggtca ccatcggcac aggaattcga tcctaagtgc
     3481 gagcacctca tcgcaccatt cgatctcacc gacaacattc caaagctgat tgagcttaca
     3541 acatccatta ccctcagcga aacagtttcc gccaacgaca atacggcaaa tggcagcaaa
     3601 ggcggacgcc atggactcaa acacaacatt ggacttgcca ctcgccgagc cgcggcaaga
     3661 ctaaactggc taccgatgga gctcgaagtg ctttacggcg aatacatact tgacaagaga
     3721 aaagactctc taataagtga aaactcaaaa gccgggcgcg cactttatcc aaaagcagag
     3781 gcattgatag acagcgcgct tggccccatc aaagagccca ctccctacaa attcaaagtt
     3841 cacgtcagca gcagcgccgg ggaaaatgcc gaagcattgc caggcggatt catcgttata
     3901 gacaaagcgc tcctcgaaag acatgaatta aaaggaaagg cacagtttgc tgtctcccat
     3961 gaaattgcac atgttctaca gcgtcatcaa acccgtgcaa tgcaagccca aattattgac
     4021 acggtatctc tcacctccaa cgtcacagaa ctatataaag tcataaagga ctccaaagaa
     4081 aaaccaaagg aggttctcaa atatatccta gcaggaaaac tcatatttca gaaacacttt
     4141 gaagaccagg agttgcaggc cgatgcttgt gcagctcgga ttctcgatac cacttttaaa
     4201 aatcgtacta atgtagtctc ctccatccga tctttcgtaa gcaggctccc tcccccagaa
     4261 ccagaagtca cgaaagacga tgaggccacc gctcttttca aagtcgtcac acgccccatc
     4321 gatcaacatc cgaccaccaa tgcgcggata gcaaatttga attttatggt caaggaaata
     4381 tatggtggaa aagccaagtc aaagtcatag cgctcaatca aatcatgcgg attcactact
     4441 tttgctccac gtgtacgacg catcgcggat acactatttc gtcgcccctg caaatctccg
     4501 ccgccccagt cccaccgcgc ctttcacgcc cgatccccct tgctcaatct cccactttcc
     4561 cttccaatcc ccctcgattc ctggaagatt ccgaggccct
//
LOCUS       DAOQLC010000228         4574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_8989_length_4574_cov_22.730250, whole genome shotgun sequence.
ACCESSION   DAOQLC010000228 DAOQLC010000000
VERSION     DAOQLC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8989_length_4574_cov_22.730250"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PL000_20735"
     CDS             complement(<1..779)
                     /locus_tag="PL000_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HNC91364.1"
                     /translation="MKKFLVKPESKINLEKIDPNDKGNFKGGKEEGLAKLEKLNSKLE
                     ALQELLFAEHKHKVLIVLQAMDAGGKDGTIRRVFDSVNPAGVRVAAFKAPTAEELDHD
                     FLWRIHKQVPGKGEMVIFNRSHYEDVLVVRVHNIVPPEVWKKRFDQINEFERTLAENG
                     TTILKFYLHIDMEEQKERLQARLDDPTKRWKFRLGDLEERKLWPQYMEAFEDVLSKTS
                     TEYAPWYIIPANRKWFRDLAISTILVDTLEDLKMKYPESEE"
     gene            complement(812..1474)
                     /locus_tag="PL000_20740"
     CDS             complement(812..1474)
                     /locus_tag="PL000_20740"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HNC91365.1"
                     /translation="MKSSPHPRLAILNLLCIAALLTFGFAPFKDGDETYWDRQLRLRG
                     KMNLPVKTLSQAKYTSCGEAVITMAYNYAYPETQITEREVIEFALAEGYYTEDQAPFT
                     SPDDMVKIAGQYASLVSSGTVTNAEEALALLTDKLTSGDPVIIDTLSRLNDPNSGAHF
                     VIVTGLTIDKNPNRTKIHFNDPLTGTNRSAYWLGIEGVWHGWQNNGDPGGAGWWMVLS
                     SP"
     gene            1523..1885
                     /locus_tag="PL000_20745"
     CDS             1523..1885
                     /locus_tag="PL000_20745"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC91366.1"
                     /translation="MKIKATNTILYCKKWRETVDFYRDGLKLLPLSSTDWFVEFKLNE
                     SARLSVADETRTSIKSGEGHGLTISLQVADVDELRAELIQSGFAPTPIKEVWGSQAMY
                     LRDPEGNRLEFWSGRAKA"
     gene            1958..2914
                     /locus_tag="PL000_20750"
     CDS             1958..2914
                     /locus_tag="PL000_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91367.1"
                     /translation="MKNVKWIAVFFAILSVLILVGIAWSGYGLLRVPEKHLGNPDILL
                     PILVSVSSIGLLVVLAFLAVSFSVLDLADRSQALGLPEGSVRALISLLLIMMFVITSI
                     FLYRQIQFNEEGVQIMDYIGISEDMLNDIPNEEIVAVTVKNNGDEQVFDVKRRIEAVN
                     QEASQRFAEQVLTTISTLVVSVSAFYFGSRSVSVARGTVSSASSPTIRSINLTEGRQG
                     DAAKDVEILGKNFDSPKTVKLVRDSDEIPIEEISASETRIRGKFAIPAGINAGKYDLV
                     VVNKDGGEDRLVNAFEVKKKEPPAQEPVEVTSPTQDPAAAQG"
     gene            complement(3025..3219)
                     /locus_tag="PL000_20755"
     CDS             complement(3025..3219)
                     /locus_tag="PL000_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91368.1"
                     /translation="MNEKAQVNVTDGAQCRVVNGVHAGKAGTIRDIKTGKTGHISITV
                     VQADGERFKTLAKNVVVVKA"
     gene            complement(3278..>4574)
                     /locus_tag="PL000_20760"
     CDS             complement(3278..>4574)
                     /locus_tag="PL000_20760"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HNC91369.1"
                     /translation="FLHGYNHSMPNTSHPLTRNAQTALNNAKRIAEQNGQSSVDSLAL
                     LLALLQLPRSQVAAVLMVLKVRVENLIARVSATIKLEAGQAATGSGEKRGGLGLSAET
                     ESIMSESIVEMKEQGLDAIDTTTLLLGMLRSPESQAGQILAQYGITAEQVHAGMKSIK
                     EMPIDRVPASELFKTLGRAMRNGISPIFISLILFTISMAGFLWFRIGSNPQVFMFAFI
                     IGGWLVSLCLHEFGHAITAFWGGDESVENKGYLTLNPLKYTHPIISVVIPLAMLLMGG
                     LAFPGGAVYINIHALRKPGYRSLVSAAGPLANLLCLFLLALPFANIPFYNFFLQAPGE
                     FLSALGLLALLQMIALFINLLPIPGLDGFGILEPFLPREWLGFASFLRPFGFLIVFFL
                     LSTDSPISDFFWSNVWSAMELLSFNLAYFANEGVKTFFL"
BASE COUNT         1060 a         1095 c         1224 g         1195 t
ORIGIN      
        1 ttttcttctg attcggggta tttcattttc aagtcttcga gggtatccac caaaatggtg
       61 gagatcgcca gatcgcggaa ccatttgcgg ttggcaggga tgatatacca cggggcatat
      121 tctgtgctgg ttttgctcaa cacatcttca aaggcttcca tatattgcgg ccagagtttg
      181 cgctcttcca gatcgccaag gcggaacttc cagcgtttgg tgggatcatc gagacgggcc
      241 tgcaatcttt ccttttgttc ttccatgtct atgtgcagat agaacttcaa aatggtggtg
      301 ccgttttcgg cgagggtgcg ttcgaattcg ttgatctggt cgaagcgttt tttccacacc
      361 tcaggcggga caatgttgtg gacacgcaca accaatacat cttcataatg actgcggttg
      421 aagatgacca tttcgccttt gcccggtacc tgcttgtgga tgcgccacag gaaatcatgg
      481 tcgagttctt cggcggtggg cgctttgaac gcggctactc gcacgcctgc ggggttgacg
      541 ctgtcgaaca cacggcggat cgttccatcc tttccgcctg catccatggc ctgtaacacg
      601 atcagcacct tgtgtttgtg ctcggcaaac aacaattcct gcagggcttc cagcttgctg
      661 ttcagttttt ccagtttggc gagtccctcc tccttgccgc ctttgaaatt gcccttgtcg
      721 ttcgggtcga tcttctcgag attgatcttg ctttcaggtt tgaccagaaa ttttttcatg
      781 aactgcctcc agggttgatg tgacttgcgg ctcacggcga tgacaacacc atccaccagc
      841 ctgcgccgcc ggggtcaccg ttgttttgcc agccatgcca gacaccttca atgcccagcc
      901 aatatgcgga gcggttggtg cctgtcaatg ggtcgttgaa gtggattttg gttcgattgg
      961 gatttttgtc gatcgtcagc ccggtgacaa tgacaaagtg cgcgcccgaa ttcgggtcgt
     1021 tgagccgtga aagggtgtca ataatgacgg ggtctccgct ggtcagttta tcggtcaaca
     1081 gggcgagcgc ttcttctgca ttggtgacgg ttccgcttga gacaaggctg gcatattggc
     1141 cagcgatttt gaccatgtca tcggggctgg tgaagggagc ctgatcttcc gtgtaatatc
     1201 cctcggcaag cgcaaactcg atcacttccc gctccgtgat ctgggtctca gggtaggcat
     1261 agttatatgc cattgtgatg accgcttctc cgcaggaagt gtattttgcc tgtgagagcg
     1321 ttttcacggg gagattcatt ttcccgcgca ggcgcagttg tcggtcccag taggtttcgt
     1381 cgccatcttt aaagggggca aaaccaaagg tcagcagggc ggcgatgcag agaaggttca
     1441 ggattgcgag tctggggtgg ggggagcttt tcatgggaaa tttgcagttg taaatattca
     1501 gattgggtac tattatacag ccatgaaaat caaagcgacg aatacgattt tatattgcaa
     1561 aaaatggcgc gagacggtgg acttttatcg tgacggtttg aagctgctgc ctctttccag
     1621 cacagactgg ttcgtggaat tcaagttgaa cgagtcagcg cgtttgagcg tggccgatga
     1681 aacgcgcact tccatcaaga gcggtgaggg gcatggcctg accatcagcc tgcaagtagc
     1741 cgatgtggac gaattgcgcg ctgagttgat tcagtcgggt tttgcgccta caccgatcaa
     1801 agaagtgtgg ggatcacaag cgatgtattt gcgcgaccct gaaggaaaca ggctcgaatt
     1861 ctggtcgggc cgcgcaaaag catgaaagat gttctctttc acctgccaat tgattttttt
     1921 tcaaatcgct gtatgatgaa attggaagga ggaagagatg aaaaacgtaa aatggattgc
     1981 tgtgtttttt gccattctgt ccgttttgat tttggtggga attgcatgga gtgggtatgg
     2041 gcttctgcgt gtgcctgaaa agcatttggg taaccccgat atcttgcttc caattttggt
     2101 gtctgtcagt tccattggcc tgctggtggt tcttgctttt ttggcggtct cattttcggt
     2161 tttggatctg gctgatcgca gtcaagccct ggggcttcca gaaggcagtg tgcgagcgtt
     2221 gatctccctg ctgctcatta tgatgtttgt gatcacatct atttttctct atcgccagat
     2281 tcagttcaat gaagaaggtg tccagattat ggattacatc ggcatttcgg aagatatgct
     2341 gaacgacatt ccaaatgaag aaattgttgc tgttacagta aaaaacaatg gtgatgagca
     2401 ggtgttcgat gtcaaacgca gaattgaggc tgtgaatcag gaggccagtc agcgttttgc
     2461 cgaacaggtg cttaccacga tcagcacact tgtggtttct gtttcggctt tctactttgg
     2521 ttcacgatct gtatctgtgg cgcgcggaac tgtatcctcc gcctcttctc caaccattcg
     2581 cagtatcaac ctgaccgaag gcaggcaggg agatgccgcc aaagatgtgg agattctcgg
     2641 gaaaaatttc gattcgccca aaaccgtcaa actggttcgt gattctgacg aaatcccaat
     2701 cgaggagatt tctgcaagtg aaaccagaat ccgcggaaag tttgcaattc ctgctggcat
     2761 aaatgctggg aaatacgatc tggtcgtggt caacaaagac ggtggtgaag atcgccttgt
     2821 aaatgcgttc gaggtcaaga agaaggaacc ccctgcacaa gaaccagtgg aggtgacttc
     2881 gccgacgcag gacccagccg cagcgcaggg atgaatttct ttaatttctc ggttttgtaa
     2941 aaaaaattaa atcataaaaa gagacagtca tcttttgggg caaaggtgac tgtctctttt
     3001 tcttccacca gagattgaat gtgtttatgc cttgaccaca accacgttct tcgcaagggt
     3061 tttgaatctt tctccgtcgg cctgcacaac cgtaatggat atgtgacccg tctttcctgt
     3121 cttgatatcc ctgatcgtac cagccttccc tgcatggacg ccgttcacaa ccctgcattg
     3181 tgctccatct gttacgttaa cctgagcttt ctcattcatg gggtatctcc ttttcctggt
     3241 ttgattggat gtacggataa attttcaaaa acagccatta gagaaagaac gttttgacac
     3301 cttcattggc aaagtaagcc aggttgaaac tgagcaattc catcgcggac cagacattgc
     3361 tccagaagaa gtctgagatc ggcgaatctg ttgagaggag aaagaacaca attaagaagc
     3421 caaaaggccg caggaaactg gcgaatccca gccattcacg cggaagaaaa ggttcaagga
     3481 tcccaaatcc atccagcccg gggatcggca gcaaattgat gaacagggcg atcatttgca
     3541 gcagggccag aagcccaagc gcggagagga actcgcctgg cgcttgcaaa aagaaattgt
     3601 aaaagggaat gttggcaaac ggcagcgcaa gcaaaaagag acaaagcagg ttcgccagcg
     3661 gacctgccgc cgagacaagg ctgcgatagc ctggttttct caacgcatgg atattgatgt
     3721 acaccgcccc gcctggaaat gcaagtccgc ccatcaacag cattgccaga gggatgacca
     3781 cactgatgat cgggtgtgtg tatttgagcg gattcagggt aaggtagccc ttgttttcca
     3841 cgctttcatc tccgccccaa aatgctgtga tggcatgtcc gaactcgtgc aggcaaagcg
     3901 aaacaagcca gccgccgatg ataaaagcga acatgaagac ctgcgggttg cttccaatgc
     3961 gaaaccacag gaaccccgcc atgctgatgg tgaacaaaat caggctgatg aagatcgggc
     4021 tgatgccgtt gcgcatggct cgccccagcg ttttgaacaa ttcagaggca gggactcggt
     4081 caatgggcat ttctttgatg ctcttcatgc cagcgtggac ctgctccgcg gtgatcccat
     4141 attgagccag tatttgccca gcctgactct cagggctgcg caacataccc agcaacaggg
     4201 tggtggtatc aatggcatca agtccctgtt ccttcatttc gacgatggat tcgctcatga
     4261 tggattcggt ttcagcagat agccccaacc ctccgcgctt ctctcccgac cctgtagctg
     4321 cctgccctgc ttcgagtttg atcgtggcgg acacacgcgc aataagattt tccactctca
     4381 ctttgagaac catcaacacc gcagccactt gtgacctggg gagctgcaac agcgccagca
     4441 gcaacgccag cgagtcaacc gatgactgtc cattttgttc ggcaatgcgc tttgcgttat
     4501 tgagcgcagt ctgtgcgttc cgtgtcagtg ggtgagatgt gttgggcatg gagtgattat
     4561 acccgtggag aaat
//
LOCUS       DAOQLC010000229         4441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9443_length_4441_cov_24.075695, whole genome shotgun sequence.
ACCESSION   DAOQLC010000229 DAOQLC010000000
VERSION     DAOQLC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9443_length_4441_cov_24.075695"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PL000_20765"
     CDS             complement(<1..649)
                     /locus_tag="PL000_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HNC91370.1"
                     /translation="MANKKVRVAIIGVGNCASSLVQGVQFYKNAKEDETIPGIMHTQL
                     GEYHVRDIEFTLAIDINATKVGKDLSEAIFAEPNNTYKFCDVPHLGVPVVRGMTHDGL
                     GKYLSEVIKKAPGSTAEISKLLKETKTDVVINFLPVGSEMATKWYVEQILEAGCAFVN
                     GIPVFIASSEYWGKRFADAGLPILGDDIKSQVGATIVHRVLTSLFVDRGVKIDRTY"
     gene            767..2026
                     /locus_tag="PL000_20770"
     CDS             767..2026
                     /locus_tag="PL000_20770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNC91371.1"
                     /translation="MDQVTLLARLLEVTRNLSTMVDVDAYLQSILSAATELTGSETAS
                     LMEYDETTQEFFFRFVPWFHRDLIKDARVPLNGSAAGWSLLHSKPLIINDVKNDERHF
                     NKVDEMANFVTRSMLVVPLLLHGRPMGVFEVINKANDAPYTDEDILALETLASLATTA
                     IQNNLLENSIQSSQNESRDLDRLKNEFIAITSHELRTPLGLILGHATFLRELVGNDFQ
                     EQVEAIIRNASRLKEIIESLSTVDNYQTGGASLRSRRVSMNRVIEDVIASFRDIAKKK
                     GVSLKSDLDRNQELLVDADGSKIAIVLSNLVKNAVTFTNDGGHITVRGSLDSSFVKVV
                     VEDDGVGIPARDLPHVFERFYQVESHLTRRHGGMGLGLSVAKVMIEMHGGRIWAESLE
                     GLGSKFTFLLPLHPKVNEPDPVSPFVE"
     gene            2123..3310
                     /locus_tag="PL000_20775"
     CDS             2123..3310
                     /locus_tag="PL000_20775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC91372.1"
                     /translation="MQSINEQFELIRQSATVAMADRILALKASGRDIVGLQVGDPDFG
                     THPAIAEAAMKAIQSGLTHYGPSRGYPELRTAVANKLMRDEGVTYDPASEILITHGGI
                     HAYYLAMQSILNPGDDVLIPDPSWATHSNMAIMLRGNVIRIPAPAENGFIPHFESWLK
                     ALTASAKPRVIVLNYPSNPTGAYPTRDYLQKLQDFAKEHSLWIVSDEVYGSLFYEGRP
                     TSAAAIEGAKERTLIINSLSKSYAMTGWRVGFLAAPAHIIENALKAGQNSITCVAPFI
                     QKAAAFALTDPAMQEFTEEMRTGYSRRRDLVLRLSHQLESEKVKVIPPQGAFYFFLDL
                     RALNMSSVEMCEKILDEVGVGLVPGSAFGEQGEGFIRMTIAASDADVEAGFRKIVEWA
                     ERQ"
     gene            3323..4135
                     /gene="pheA"
                     /locus_tag="PL000_20780"
     CDS             3323..4135
                     /gene="pheA"
                     /locus_tag="PL000_20780"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC91373.1"
                     /translation="MKVSFQGEPGAYSEQAVFNYFGEVETVPCESFDVMFDSVVSGKS
                     DVALAPIENSLAGSIHQNYDLLLRHDLHIVGEYFLRVRHCLIAMPGAKMEDIKKAISH
                     PQALGQCAAYLRGRGIKPETVYDTAGSVKMLKASGAMDTAAIASRRAAELYGMQILEE
                     GIEDNSENYTRFLAVQREAAFPKGEAKTSIVFTLKHVPGSLFKAMSVFALRDIDLTKI
                     ESRPLQGKPWEYLFYIDFIGSADDDTPRRALEHLGEYATTLRVLGSYPRFKG"
     gene            complement(4125..>4441)
                     /locus_tag="PL000_20785"
     CDS             complement(4125..>4441)
                     /locus_tag="PL000_20785"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HNC91374.1"
                     /translation="DAKLDVQVNGSFSVTFANSDGAEFTAQGIYKEVNEPNRLVFTWG
                     WANQPEVHELISLEFSPDGDGTLMTFEQSNIDPSTSMHNYEEGWRSTFQKLKKALGTI
                     NP"
BASE COUNT         1070 a         1107 c         1136 g         1128 t
ORIGIN      
        1 gataggtgcg gtcgatcttc acgcctcgat ctacgaagag gctggtcagc acacgatgaa
       61 cgatcgtcgc cccgacctga ctcttgatgt cgtcaccgag gatgggcagg cccgcatcgg
      121 caaaacgctt gccccaatat tcagaagaag cgataaagac ggggatgcca ttaacaaatg
      181 cgcagcccgc ttcaagaatc tgctccacat accatttggt tgccatctcg gaaccaacgg
      241 gcaggaagtt gatgaccaca tcggtcttgg tttccttgag caattttgag atttcagcag
      301 tggatcccgg tgcttttttg atgacttcac tcaaatattt gccgaggccg tcatgggtca
      361 tgcctcgcac cactggtacg ccgaggtggg ggacatcgca gaatttgtag gtgttattgg
      421 gttcggcgaa gatggcttct gaaaggtcct tgccgacctt ggtggcattg atatctattg
      481 ccagtgtgaa ttcaatatca cggacgtggt attcgccaag ctgggtgtgc ataatgcctg
      541 ggatggtttc atcctctttt gcgttcttgt agaactgaac accctgcaca agcgaagagg
      601 cgcaatttcc cacgccaatg atcgcaacgc gtactttttt gtttgccatg gttgtttctc
      661 cttttcgttg tttaattaca atttccttaa gcctgctgtt tgcctgccac tacagataga
      721 cctgtgaagt ataatcttac tgtaagatat gtaggtaatc atggccatgg atcaagtgac
      781 actacttgca agactattgg aagttacacg caacctcagc acaatggtgg atgtggatgc
      841 gtacctgcaa tcgattcttt cggcggcaac cgagttgact ggcagcgaaa ccgcttccct
      901 gatggaatac gacgaaacca cgcaggaatt ctttttcagg ttcgtgccat ggtttcaccg
      961 tgacctgatc aaggatgccc gtgtcccatt gaatgggagc gccgctgggt ggtcgctttt
     1021 gcactccaaa cccctgatca tcaatgacgt aaagaatgac gagcggcact tcaataaagt
     1081 ggatgagatg gcgaattttg tcacccgctc catgctggtt gtgccgcttt tactgcatgg
     1141 caggccgatg ggggtcttcg aggtcatcaa taaagccaac gacgctccat acactgatga
     1201 agatatcctc gctttggaaa cccttgcttc actggctact actgccatac agaacaatct
     1261 gcttgaaaac agcattcaat cttcccaaaa cgaatcacgc gaccttgacc gtctaaagaa
     1321 cgaattcatc gcgatcacct cccatgaact gcgtacaccg ctgggcttga tcctcggcca
     1381 tgccacgttt ttacgtgaat tggtggggaa tgattttcaa gaacaggtgg aggccattat
     1441 ccgcaatgct tccaggttga aggagatcat tgaaagcctc tccactgtgg ataattacca
     1501 aacaggcggt gccagtctcc gttcgcgcag ggtttccatg aaccgcgtca ttgaagatgt
     1561 cattgcttcg ttccgcgata ttgccaagaa gaagggcgtt tcattgaagt cggacctcga
     1621 ccgcaatcaa gagctgctcg tggatgcaga tggcagcaag atcgccatcg tattgagcaa
     1681 cctcgtcaag aatgccgtca cctttaccaa tgatggtggt cacattacag tacgcggcag
     1741 ccttgactcg agttttgtaa aagtagtcgt tgaagatgac ggcgtgggca tccctgcccg
     1801 tgacctgccg catgtatttg agcgttttta tcaggtcgaa tctcatctta cgcgcaggca
     1861 tggcggcatg gggctgggcc tgtctgttgc aaaagtgatg attgaaatgc atggcggacg
     1921 catttgggcc gaaagcctcg aagggcttgg cagcaagttc accttcctgc tgccgttgca
     1981 ccccaaggtg aatgagcccg accctgtttc acctttcgtt gaataaaaaa cgatctctcg
     2041 aatttcccaa tcccgtcaaa tgagttgacg ggattttgat tcctttgata cactcgctcc
     2101 attaaatcgc aaaggaaaac ccatgcaaag cattaatgag caattcgaat tgattcgcca
     2161 atctgccact gttgccatgg cagaccgtat tctggcactc aaagccagtg gccgtgatat
     2221 tgtcggtttg caagtcggtg accccgattt tggaacacac ccagccattg ccgaagccgc
     2281 catgaaagcc attcaaagcg gattgacgca ttatggccct tcacgcggtt atcctgaact
     2341 gagaactgct gttgccaata aactgatgcg ggatgaaggc gttacttacg accccgccag
     2401 tgagatcctg attacacatg gcggcatcca tgcctattac cttgccatgc aatccatttt
     2461 gaatccaggg gatgatgtgc tgattcctga cccgtcctgg gcaactcatt cgaacatggc
     2521 gatcatgctg cgcggaaatg tcatccgcat ccctgccccc gccgaaaatg gattcatccc
     2581 ccattttgaa tcgtggctga aagcgttgac cgccagcgcg aaaccccgcg tcatcgttct
     2641 caactatccc tccaacccaa caggggcata tcccacacgc gactacctgc aaaagctgca
     2701 agactttgcc aaagaacaca gcctgtggat cgtgagcgat gaagtgtatg gcagtctgtt
     2761 ctacgaagga cgtccgactt ctgcagcagc gatcgaaggt gcaaaagaac gcacactgat
     2821 catcaacagc ctttccaaat cctatgccat gacgggctgg cgggtgggat tccttgccgc
     2881 ccctgcccat attattgaga atgctctcaa ggcggggcag aattccatta cttgtgtagc
     2941 ccccttcatt caaaaagctg ctgcctttgc gctaaccgac ccagccatgc aggaattcac
     3001 cgaagaaatg cgaacgggct attcccgccg cagggatttg gtactgcgcc tgtcacacca
     3061 gttggaaagc gagaaagtga aagtgatccc tccgcaggga gcattttatt ttttccttga
     3121 tctgcgcgct ttgaatatgt cttctgtgga aatgtgtgaa aagattttgg atgaggttgg
     3181 cgtggggctg gtgcctgggt ccgcttttgg cgagcagggc gaaggtttta tccgcatgac
     3241 cattgccgcc tccgatgcag atgtggaagc agggtttagg aagatcgtgg aatgggccga
     3301 aaggcagtaa ttggagatga acatgaaagt atcttttcag ggtgaacctg gcgcgtattc
     3361 tgaacaggcg gtctttaatt attttggcga agtggaaacg gttccatgtg aatcatttga
     3421 tgtgatgttc gattcggtcg tttcaggcaa aagcgacgtg gctttggctc ccattgaaaa
     3481 ttcactggcg ggtagtattc accagaatta cgacctgtta ttgcggcatg acctgcatat
     3541 tgtgggggag tatttcctgc gcgtgcggca ttgtctgatc gccatgcccg gtgcgaagat
     3601 ggaagatatc aaaaaagcca tcagccatcc acaggcgctg ggtcaatgtg ctgcgtattt
     3661 gcggggcagg ggcatcaaac ctgaaaccgt gtacgataca gcagggagcg tgaaaatgct
     3721 caaggcttca ggagccatgg atacagccgc cattgcatca cgccgtgcgg ctgaattgta
     3781 cggaatgcag atcctggaag aggggatcga agacaatagc gaaaactata cgcgtttcct
     3841 cgctgtccaa cgagaggcag ctttcccgaa gggcgaagcg aagacttcca tcgtgtttac
     3901 attgaagcat gtaccgggat cgcttttcaa ggcgatgtct gtttttgcct tgcgcgatat
     3961 tgacctgacc aagatcgaat cacgcccgtt gcagggcaaa ccgtgggaat atctttttta
     4021 cattgatttc atcggttccg ccgatgatga caccccaaga cgggcgcttg agcatttggg
     4081 tgagtacgcc accaccttgc gcgtgttggg gtcgtatcca cgtttcaagg gttgatcgtt
     4141 cccaacgctt tttttaattt ttgaaaggta ctgcgccagc cttcttcata attatgcatt
     4201 gacgttgaag ggtcaatgtt cgattgctca aaggtcatga gagttccatc gccgtctgga
     4261 gaaaattcca atgaaatcaa ttcatgtact tcaggttggt ttgcccagcc ccacgtgaaa
     4321 acgagacggt tcggttcatt gacctctttg taaattccct gcgctgtaaa ttcagcgcca
     4381 tcggaattgg caaaggtcac cgaaaacgat ccattgactt gcacatcaag tttcgcatcc
     4441 a
//
LOCUS       DAOQLC010000230         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9692_length_4371_cov_26.776645, whole genome shotgun sequence.
ACCESSION   DAOQLC010000230 DAOQLC010000000
VERSION     DAOQLC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_4371_cov_26.776645"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="PL000_20790"
     CDS             complement(<1..824)
                     /locus_tag="PL000_20790"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNC91375.1"
                     /translation="MTLSFIFGTVGSPTGTPKKPGGSVGAIAFSKSIGLHALELGWVQ
                     SVRVTEATCAAIKSASEEQGVSLSVHAPYFINLNANAEEWPKSRKRLMDAAHYGYLAG
                     ATDIIFHPGSYFENDPLEVLKVAIPRLKGCVDELKKNGDHVTLRPETMGKSAMLGSFE
                     DAVAMSAAMDMVQPCIDFAHMHARPGDGTMNTYEEWSKLLTAYKKELGAKALKHMHIH
                     LSGIEYGPKGEKNHLPLAEADLDLKALFKALHDFGCGGRILCESPIMEEDALNMKKA"
     gene            complement(840..1229)
                     /locus_tag="PL000_20795"
     CDS             complement(840..1229)
                     /locus_tag="PL000_20795"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNC91376.1"
                     /translation="MKAFFESRIASFRYAFQGWFHVLKTQRNAWIHSAIATIVFLLGL
                     WLQLPPRDWAVIILTAALVFTAEFINTSIEAVVDLASPDRHPLAKIGKDVGAAAVLVS
                     ALAAILIGLLLLGPPLWQKLTLLFSNL"
     gene            complement(1235..1708)
                     /gene="ybeY"
                     /locus_tag="PL000_20800"
     CDS             complement(1235..1708)
                     /gene="ybeY"
                     /locus_tag="PL000_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNC91377.1"
                     /translation="MINIDNQQDFRESALLERAARLTLEISTRLDPNLEPDSVDADIT
                     IVLTDDAQLHELNREFLGVDAPTDVLSFPSDEVDPETGSQYLGDIIISIPRAAQQAQV
                     GGHSLEAEVQLLVVHGTLHLLGHDHAEAEEKARMWAAQAEVMGRLGLSHVKIQDT"
     gene            complement(1705..3822)
                     /locus_tag="PL000_20805"
     CDS             complement(1705..3822)
                     /locus_tag="PL000_20805"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNC91378.1"
                     /translation="MSARTRTPVTTRIRVLQISLIIAVGLASFAALTMPIALRPSTQA
                     LDVGDVSQNTLQAPRDIEYVSDIRTEEARKAAESAVQPVYTPPDPAIARQQIERLRTT
                     LQYINSVRTNVGMTTDEKKSNLVELSDVRLKLETIDYLVGISDARWEIVQSEALRVLE
                     QVMRRAIYEDKVESTQTGIPSIVSLTLNEQQSALVAELASAFVVPNSFFSEELTIAAQ
                     QAARDAVKPIVQTYKAGETIVPAGEIITPADMEAFQQLDMIRPGQRWEDMLGAVSIIV
                     LSAVLVPLYFFRRKRAVVVNDPRSIFVIALVFVVFLVGARLFANRTLAPYGYPLQAAG
                     LLITALFGLEAGLVISIPLCLLAAYGLPNTIDLAPYYLLSSLVGLLVLGPVRRFWGFV
                     RAGIAISLTGLVVLLAYRLPFFTPDLLGVFQFIGVVSFAGFAAASVTLLLQYLLAQTL
                     GLTTAFQLLDISRPDFPLLQFFLRNAPGTYQHSLQVANLAEQAAEKIGADPLLTRVGA
                     QFHDIGKALNPTFFIENQIPGSVNKHHDIAPEESAATIIRHVTDGIQLAKKYRLPGRL
                     HDFILEHHGTLITRYQYNQAMEAANGDVTRVDIENFRYPGPRPNSRETALLMLADATE
                     ARARAERPATDDDLRALVRSVIDTVQKFGQLDDTLLTLRDLNLITESFVTTLRGTYHP
                     RIQYPNAKVPDQDSTILATRKEK"
     gene            complement(3880..4326)
                     /locus_tag="PL000_20810"
     CDS             complement(3880..4326)
                     /locus_tag="PL000_20810"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNC91379.1"
                     /translation="MDTKTQLNESVKDAMKSGDELRKRTLRMVLAAVKQVEVDKQTTL
                     DDMAVVALIQKEIKNRREAIEEAKKAERPDLIGDNEAEIKVLEVFLPKAMPAEELRAL
                     VSAAIAETGAAGPSDMGKVMKIVMPKVAGRAPNDMISSTVKELLAK"
BASE COUNT         1064 a         1225 c         1123 g          959 t
ORIGIN      
        1 gccttcttca tatttaacgc atcctcttcc atgatggggc tttcacacag gatgcgcccg
       61 ccacaaccga aatcatgcaa cgctttgaac aacgccttca aatccaggtc cgcttcggcg
      121 agcggaagat ggttcttttc acctttgggg ccatactcaa tgcctgaaag gtgaatgtgc
      181 atatgcttca atgcctttgc acccaattcc tttttatagg ccgtcaacag cttcgaccat
      241 tcttcgtagg tgttcatcgt gccatcaccg ggacgggcgt gcatgtgagc aaagtcaatg
      301 cagggctgca ccatatccat ggcggcgctc atggcaacgg catcttcaaa ggagccaagc
      361 atggccgatt tgcccatcgt ctccgggcga agagttacat gatcgccatt cttctttaat
      421 tcatccacgc agcccttcaa acgcggaatt gcgaccttca acacttcaag cgggtcattt
      481 tcaaaatacg agccggggtg gaagataata tccgttgcgc ctgcgagata gccgtaatgt
      541 gccgcatcca ttaagcgttt gcgtgatttg ggccattcct cagcatttgc attcaagttg
      601 atgaaatacg gcgcatgcac gctcaacgaa acgccctgtt cttcgctggc cgatttgatc
      661 gccgcacagg tggcctcggt cacacgcacc gattgcaccc agcccagttc caatgcatgc
      721 aacccaatgg atttactgaa agcgatcgcg cccacagaac caccgggctt ttttggggtg
      781 cctgtgggtg aacccactgt accgaaaatg aacgataagg tcatgaaagt ctccagagat
      841 taaaggttag aaaacaggag ggtgagtttt tgccagagag gcgggcccag taaaagcagg
      901 ccgatcaaga tcgccgccaa agcagaaacc agcaccgccg ccgcgccaac atctttgcca
      961 attttagcaa gcggatgccg atcagggctg gccagatcca ccacggcttc aatggaagtg
     1021 ttgatgaatt cagccgtaaa aacaagcgcc gctgtaagaa tgatcaccgc ccaatcgcgc
     1081 ggcggaagtt gcagccacaa tcccagcaaa aagacgatgg tcgcaatcgc actgtgaatc
     1141 cacgcattgc gctgggtttt caaaacatgg aaccacccct gaaacgcgta acggaacgac
     1201 gcgatccgcg attcaaaaaa cgccttcact ttgattatgt atcctgtatt ttgacatgag
     1261 acaagccgag tcttcccata acctcggcct gggcagccca catacgggct ttttcctccg
     1321 cctcagcatg atcgtgaccc agcaaatgca acgtaccatg caccaccagc agctgcactt
     1381 ccgcttccag cgagtgcccc cccacctgtg cctgttgcgc cgcgcgggga atcgaaatga
     1441 tgatatcgcc cagatattgc gaccctgttt cggggtccac ctcatcggat ggaaaagaca
     1501 gcacatcggt gggagcatcc acaccgagga actcgcgatt cagttcgtgc aattgcgcat
     1561 catctgtcaa aacgatggtg atgtctgcgt ctacagaatc aggctcaagg ttgggatcga
     1621 gccgagtcga aatctcaagt gtgaggcggg cggcccgttc gagcagggct gattcccgaa
     1681 aatcctgttg gttatctatg tttatcattt ttcttttcgt gtggcgagaa tggttgaatc
     1741 ctggtctggc actttggcat tggggtattg aatgcgcgga tgataggtgc cgcgcaacgt
     1801 ggtgacaaat gattcggtga tgaggttcag atcgcggaga gtcagcagtg tgtcatccaa
     1861 ttgaccgaat ttttgcacag tgtcgatcac gctgcgaacc aatgcgcgca gatcgtcatc
     1921 ggtggcgggg cgttcggcgc gggcgcgggc ttcggtggca tcagcgagca tgagcagggc
     1981 cgtttcacgc gaatttgggc gcggcccggg gtagcggaaa ttttcgatat ccaccctggt
     2041 cacatcgcca ttggcggctt ccattgcctg gttgtattga taacgtgtga tcaacgtgcc
     2101 gtgatgttca agaataaaat catgcaggcg gcccggcagg cgatactttt tcgccagttg
     2161 aatcccatcg gtgacgtgtc gaataatggt cgccgcagat tcttcggggg caatatcatg
     2221 atgtttgttg acgctccccg gaatttggtt ctcaatgaag aacgtggggt tgagcgcctt
     2281 gccaatatca tgaaactgcg caccaacccg ggtcaatagc gggtcagcgc cgatcttttc
     2341 agcggcctgc tcggcgaggt ttgcaacctg caaactgtgt tgataggtgc ccggcgcgtt
     2401 gcgcaaaaag aattgcaaca gcgggaaatc gggccgcgag atatcgagca gttgaaatgc
     2461 tgtggtcagc ccaagggttt gcgccaacag gtattgaagc aaaagagtca cactggcggc
     2521 ggcgaacccg gcaaaagaca ccaccccaat aaattgaaac acacccagca gatcgggtgt
     2581 aaagaacggc aggcgatatg ccagcagcac caccaacccg gttaatgaaa tggcaatgcc
     2641 tgcacgcaca aaaccccaaa atcggcgcac gggacccagc acaagcaaac ccacaagaga
     2701 agaaagcaaa taataaggag ccaggtcaat ggtattgggc aggccatagg cggccagcaa
     2761 gcataatgga atggaaatga ccagccctgc ctcaaggcca aacaaggctg taatcaacaa
     2821 ccccgccgcc tgcagtggat atccatacgg tgcaagtgta cggttggcaa acagacgcgc
     2881 cccaaccaga aacaccacaa aaacaagcgc aatgacaaaa atgctgcgcg gatcattcac
     2941 caccaccgca cgcttcctgc ggaagaaata caaaggcacc aacaccgccg aaaggacgat
     3001 gatcgaaacc gcgccgagca tatcctccca gcgctgaccg gggcggatca tatccagctg
     3061 ttgaaaggct tccatgtctg caggtgtgat gatctctccc gcaggcacga tcgtttcccc
     3121 ggctttgtag gtttgcacaa tcggcttgac cgcatcacgt gcggcctgct gtgctgcaat
     3181 ggtcaattcc tcgctgaaaa aactattcgg caccacaaag gcacttgcca gctccgccac
     3241 cagcgccgat tgctgttcat tcaacgtcag actcacaatg gaaggaatgc ccgtctgggt
     3301 ggattccacc ttgtcttcat aaatggcacg gcgcatcacc tgttccagca cacgcaatgc
     3361 ttcagattga acgatctccc agcgtgcatc agaaataccg accaggtaat caatggtttc
     3421 gagcttcaag cgcacatcgc tcaactccac cagattcgat ttcttttcat ctgtggtcat
     3481 tcccacattg gtccgcactg agttgatata ttgcagggtc gtccgcagcc gttcgatctg
     3541 ctgacgcgca atggcaggat cgggcggcgt atacaccggc tgcaccgcgc tttccgccgc
     3601 cttgcgggcc tcctccgtgc gaatgtcact cacatattca atatcacgcg gagcctgcag
     3661 ggtattctgc gaaacatcgc caacatccag cgcctgcgtc gatggacgca gtgcgatcgg
     3721 cattgtcagt gcagcaaagg aagccagccc gaccgcaatg atcaggctga tctgtaagac
     3781 tcgaatacgg gtggtaacgg gggtgcgggt acgtgcagac ataaaataag ccgcaaaggg
     3841 gcaggtgcaa ccatgcaccc acctcttcac ggcaagggct tacttcgcga gcaattcttt
     3901 cacagttgaa ctgatcatat cattcggcgc acgaccagcc accttgggca tcacgatctt
     3961 catcaccttg cccatatcgc ttggcccggc tgcaccggtt tcagcgatgg cagcgcttac
     4021 caatgcgcgt aattcctcgg caggcatggc cttcggcagg aagacctcca acaccttgat
     4081 ctcggcctca ttatcaccga tcaaatccgg gcgttccgcc tttttggcct cttcgatggc
     4141 ctcacggcga ttcttgatct ccttctggat caaagccacc acggccatat catcaagggt
     4201 ggtctgctta tccacttcga cctgcttcac agcggccagt accatgcgca gcgtgcgttt
     4261 gcggagttca tcgccgcttt tcatggcatc tttaactgat tcgttgagtt gagtttttgt
     4321 atccataaaa ttgattatac cctttcatct tattggatga aaaaccgctt c
//
LOCUS       DAOQLC010000231         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9923_length_4308_cov_14.529273, whole genome shotgun sequence.
ACCESSION   DAOQLC010000231 DAOQLC010000000
VERSION     DAOQLC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9923_length_4308_cov_14.529273"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1789)
                     /locus_tag="PL000_20815"
     CDS             complement(<1..1789)
                     /locus_tag="PL000_20815"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC91380.1"
                     /translation="MKLKFKTQAYQTAAVQAVVDCFKGQVPHHGGVRYRLDPGSQKAA
                     PASPQAALALEAASPEAAAEREAAFRNADFTLSETALLDNIRAVQHGQNLPVSDALVK
                     TKVAKVNLDIEMETGTGKTYCYIKTIFELNKQYGWSKFIIVVPSIAIREGVAKSLQIT
                     AEHFLESYHKKARFFIYNSKQLHHLESFSSDAGINVMVINVQAFAARGADNRRIYDVL
                     DDFQSRKPIDVISANRPILILDEPQKMEGAATLKSLEEFKALMVLRYSATHKTTHNKI
                     HRLDALDAYNQKLVKKIAVRGIAVKGLAGTAGYLYLQSIEISSKKPPEARVEFEQKLA
                     GGNIKRVVRKLSKGDNLFDLSNGLDQYRDGFVVADINANSDTLTFTNGVELTVGDATG
                     DVTEAALRRIQIREAIKAHFDKEQALFQQGVKVLTLYFIDEVVKYRDYSQPDEKGEYA
                     RIFEEEYQLFLNEVLELDETPYVKYLKGISAEKTHSGYFSIDKKSKRLADPSVVARGE
                     NAGLSDDVDAYDLILKDKERLLSFAEPVRFIFSHSALREGWDNPNVFVICALKHSDNT
                     ISRRQEVGRGLRLSVNQSGDRMDHPATVHE"
     gene            complement(1800..3764)
                     /locus_tag="PL000_20820"
     CDS             complement(1800..3764)
                     /locus_tag="PL000_20820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC91381.1"
                     /translation="MDKLKMHSPNLTQDNIARIRDLFPGCVTEAKGEDGNVKLAVDFD
                     QLRQELADSILEGTQERYQLNWPGKRESILAANSPIQKTLRPSRNESLHFDETKNIFI
                     EGDNLEALKLLQDNYLGRIKTIYIDPPYNTGSDLIYNDYFTEGASDFRQKASERDELE
                     NRLVTNSDTQGRYHSNWLSMIYPRLKIARSLLSDDGLIFISIGDEEQANLKKLCDEIF
                     GGSNFIATFPRITKKGGKSTDAVSKNHDYVICYSKSDAARLYPFEHSDEGFSNSDQHV
                     DERGKYKLNQTLDYDTLGYVKSLDYEIEVDGVFYYAGGVTKKEWEQRRAENPKDGYRW
                     RWSKELFNFGLANDFVVIKNGRNGARIYTKTYERATIDDGPSGGYSVVIEDRTKALPS
                     IEFIENEYSNDNAKKEVQKLIGKGMFDYPKPSSLVQKLVSFSSRNDDIVLDFFAGSCT
                     TAHAVMKANAEDGGNRRFIVIQIPEACSEKSQALKLGFKSISAIGIERIKQSGATVLA
                     GPCHSEWKKDIGFRALKVDTSNMADVYYVPDALNKAEIDLFVDNIKPNRTEEDLLFQV
                     MLDWGVDLALPIARESIQDKEVFFVDDNALAACFNAHSGIDEAFVKELAKRQPLRVVF
                     RDAGFKDDATKINVEQIFKLLSPATEVKCI"
     gene            complement(3803..>4308)
                     /locus_tag="PL000_20825"
     CDS             complement(3803..>4308)
                     /locus_tag="PL000_20825"
                     /inference="COORDINATES: protein motif:HMM:NF025693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HNC91382.1"
                     /translation="PCFGRNTVASVRWISYPSELHEDVLRCIDGAVQFPILFELNQGQ
                     GDQAKTQVVAAYKRPSEADASRWVLSSYFTSDWMPAATARTAMPLALDMAHLYSVLLQ
                     GLMPLPARPQEALPDWISRIEQAASKHREVEKTQARLAKEKQFNRKVEINATLRQLKS
                     ELQQLSR"
BASE COUNT          879 a         1175 c         1110 g         1144 t
ORIGIN      
        1 cttcgtgtac cgtggccggg tgatccatcc tgtcaccgct ttggttgacg gacagtcgca
       61 agccacgtcc cacttcctgg cggcgggaaa ttgtgttgtc actgtgcttg agcgcacaga
      121 tcacaaacac gttcgggttg tcccagcctt cacgcagggc agagtgcgaa aaaatgaagc
      181 gcacgggctc ggcgaacgac agcaaccgtt ccttgtcctt aaggatcagg tcgtaggcgt
      241 ctacgtcgtc agaaagtccc gcgttctcgc cacgcgctac gaccgatggg tcggccagtc
      301 gtttgctctt cttgtcgata gagaaatagc cgctgtgagt cttttcggca gaaatccctt
      361 tgaggtattt gacgtacggc gtctcatcca gctccagcac ctcgttgagg aagagctggt
      421 actcctcctc gaagatgcgg gcgtactcac ctttttcgtc gggctgcgag tagtctcggt
      481 acttgaccac ttcgtcgatg aagtaaagcg tgagcacctt cacgccttgc tggaacagcg
      541 cctgttcctt gtcgaagtgc gcctttatgg cctctcggat ctgtatgcgg cgcagcgcgg
      601 cctcggtcac atcaccagtc gcatccccga cggtgagttc aacgccattg gtgaaggtca
      661 gggtgtcgga gttggcgttg atatcggcca cgacaaagcc atcccgatac tgatcgagcc
      721 cgttcgacag atcgaacaag ttgtcgcctt ttgagagctt cctgaccacc cgcttaatgt
      781 ttccgcccgc cagtttttgc tcgaactcca cacgcgcttc tggaggcttc ttgctcgata
      841 tctcgatgga ctgcaggtac agataacccg ccgtgcccgc aaggcctttc accgcgatgc
      901 cgcgcacggc aatttttttc accagcttct ggttgtaggc gtccagcgca tcgagacggt
      961 ggatcttgtt gtgcgtagtc ttgtgggtgg ccgagtagcg caacaccatc agcgccttga
     1021 actcctccag agacttcagc gtcgccgcgc cttccatctt ctgcggctca tccagaatca
     1081 agatggggcg gtttgcgctg atcacgtcga tgggcttgcg cgactggaaa tcgtccagca
     1141 cgtcataaat gcgacggttg tcggcccctc gtgcggcgaa ggcctggacg ttaatcacca
     1201 tcacgttgat gcccgcatcc gaagagaaac tctccaggtg gtgcagctgc tttgagttgt
     1261 agatgaagaa gcgtgccttc ttgtgatagc tctccagaaa gtgctcggcg gtgatctgca
     1321 gcgacttggc cacgccttca cggatggcga tgctgggtac cacgatgatg aacttgctcc
     1381 agccgtactg cttgttcagc tcgaaaatgg tcttgatgta gcagtaggtc ttgcccgtgc
     1441 ctgtttccat ctcgatgtcg agattgacct tggccacctt ggtcttgacc agtgcgtccg
     1501 acacgggcag attctggccg tgctgcacgg ctcggatgtt gtcgagcagc gctgtttccg
     1561 acaacgtgaa gtcggcgttg cggaacgcgg cttccctttc cgctgcagct tcaggcgaag
     1621 cggcctccag cgcgagcgcc gcttgcgggc tcgctggggc tgctttctgg ctgccgggat
     1681 cgagccgata gcgaacgccg ccatgatgcg gaacctgccc cttgaagcaa tctactactg
     1741 cctgcaccgc cgccgtctgg taggcctgcg tcttgaattt cagcttcatc gcagcctcct
     1801 cagatgcact tcacttcggt ggcaggcgac aagagtttga agatctgctc aacgttgatc
     1861 ttggttgcgt cgtccttgaa gcccgcatcg cggaagacaa cccgcagcgg ctggcgcttg
     1921 gccagttcct tcacgaatgc ttcgtcaatg ccactgtgcg cgttaaagca ggcagccaat
     1981 gcgttgtcat caacaaagaa cacttctttg tcctgaatgg attccctggc aatcggcaac
     2041 gcaagatcaa caccccagtc cagcatcacc tggaacagca agtcttcctc tgtacggttg
     2101 ggcttgatat tgtcgacaaa aagatcgatc tcagccttgt tcagcgcgtc gggcacgtaa
     2161 tagacgtccg ccatgtttga ggtatcaact ttgagcgccc taaagccaat atccttcttc
     2221 cattcgctgt ggcatggacc tgcaaggaca gttgcgccgg attgcttgat ccgctcgata
     2281 ccaatggcag atatagattt aaaaccaagt ttgagtgctt gcgacttttc agagcaagcc
     2341 tccggaattt ggataacgat aaatcttctg tttccgccat cctcagcgtt ggccttcatt
     2401 acagcgtgag ccgttgtgca ggagccagca aagaaatcaa gaacaatgtc gtcattacgg
     2461 ctggagaatg aaacaagttt ttgcacaaga ctagatggct ttggataatc aaacatccct
     2521 ttcccgataa gcttttgaac ctcttttttt gcattgtcgt ttgagtactc attctcaata
     2581 aactcaatac ttggtagcgc cttggttctg tcctcgatta cgaccgagta gcctccacta
     2641 ggcccatcat cgatggttgc tctttcatag gtcttggtat aaatccgagc tccattcctg
     2701 ccgtttttta ttacgacgaa atcattagct aagccgaaat taaacagctc tttcgaccat
     2761 cgccagcgat atccatcttt tgggttctct gctcttcttt gctcccattc tttcttggtg
     2821 acgccgccgg cgtaataaaa aacgccatct acctctattt catagtcgag cgacttgacg
     2881 tagccaagcg tgtcatagtc gagggtctgg tttagtttgt atttacccct ctcgtcgaca
     2941 tgttgatcac tgtttgagaa cccttcgtcg ctatgttcaa aaggatacaa cctcgccgca
     3001 tctgattttg aatagcaaat aacataatca tggttcttcg atactgcatc ggtcgatttt
     3061 cctccctttt ttgtaattct tgggaaagtc gcgataaaat tcgagccgcc aaatatttca
     3121 tcacatagct tttttaaatt cgcttgctct tcgtctccaa tgcttataaa tataaggccg
     3181 tcatccgaaa gcaggcttct tgcaattttt aggcgcggat aaatcatgga tagccagttc
     3241 gagtgatatc gaccctgcgt atccgaattg gtgacgagcc tgttttctaa ttcatcgcgc
     3301 tcgctggctt tttgacggaa gtccgatgca ccttccgtga aatagtcgtt gtagatcaaa
     3361 tcacttccgg tgttataggg cgggtcgata tatatcgttt taatcctacc gaggtagttg
     3421 tcttgaagaa gcttaagagc ctctaagtta tcaccctcga tgaagatgtt ttttgtttcg
     3481 tcgaaatgca ggctttcatt gcgggatggg cgcagagttt tctggatggg tgagtttgct
     3541 gccaaaatcg actcacgctt tcccggccaa tttagctggt atctctcttg tgtcccctct
     3601 aaaatcgaat cagccaactc ctgtcgtagc tggtcaaaat ccaccgccag cttcacgttg
     3661 ccgtcttcac ccttggcttc cgtcacgcaa cccggaaaca gatcgcgaat gcgggcgatg
     3721 ttgtcttgcg tgagattggg cgagtgcatt ttcagtttgt ccattttcgg tcctctggtc
     3781 gctgcgcttc cgatatcggg gttcagcggc tcaattgttg aagttcagat ttcagttgcc
     3841 gcaaggtcgc gttgatctcg accttgcggt taaattgctt ttctttagcc agtctcgcct
     3901 gggttttctc gacttcgcgg tgcttacttg ccgcctgctc gatacgtgaa atccagtcgg
     3961 gcaaggcctc ctgtggacgt gctggcagcg gcattaagcc ttgcagcaga acggagtaca
     4021 ggtgggccat gtcgagggcc aacggcatag ccgtgcgggc agttgccgca ggcatccagt
     4081 cgcttgtgaa gtagcttgac agcacccagc gactggcgtc ggcttcgctc gggcgcttgt
     4141 aagccgccac tacctgcgtt ttcgcctgat cgccctggcc ttggttcagt tcaaacagga
     4201 tcgggaactg caccgcgcca tcaatgcagc ggagcacgtc ttcgtgtagc tcggacggat
     4261 aggaaatcca acggacgcta gcaactgtat tacggccgaa gcatggcc
//
LOCUS       DAOQLC010000232         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9927_length_4307_cov_25.339840, whole genome shotgun sequence.
ACCESSION   DAOQLC010000232 DAOQLC010000000
VERSION     DAOQLC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9927_length_4307_cov_25.339840"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PL000_20830"
     CDS             <1..1016
                     /locus_tag="PL000_20830"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNC91383.1"
                     /translation="PILVTGASGFVAIHTVIQLLQQGYNVRGTIRSLSKEADLRKTIS
                     NYVQANDRLEILSADLSQDAGWDEAMQNVETVLHVASPFPLYEPKHEDELIVPAVQGT
                     LRVLRAAHKAGVKRMVQVSSVAAISSGHNGENKTFSEADWSDLTKDIGAYAKSKTLAE
                     RAAWDFIHGSENTNKMQLTTINPPLILGPVPNKDFRTSIELVRTIMLGQVPGVGRIKM
                     GSVDVRDVASALILAMQTPEAAGQRILVAADRSLWLKEIAELLHGKYAGRYKINKLEF
                     PSILVRLIALFDKKVARVTESLDWDHELSNENAKRILKWTPRSAEEGILSMAESLIEQ
                     GVI"
     gene            1159..2601
                     /locus_tag="PL000_20835"
     CDS             1159..2601
                     /locus_tag="PL000_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91384.1"
                     /translation="MQKKLTTLIVILGNITVLSGLALLAYLGFYNRYWADDWCYSADA
                     RQLGTIPATLQYFDTEDTGYSSNRYSLTFFSALTENTLGMFGNQLVATLTISLWLVGL
                     VWTGWNVSKLIKPLPFAAILFASSLLLFYNLYLSPQRFQILYWRSGVLPYSTAIVFWM
                     LILGFVTSQINRETPARWVNYIVAPLAFLAAGLGEISCVFLFSGTTLFLLAAWYAKKQ
                     KQHWAEQSFQTLFIAWLFLLLGMIALIVSPSNARVADMNVKRSSLFSVPFVSLKHAID
                     FIVFSLKDLPLPHAIFIGIFLGFGIITTKADDSTLTVKRVAILLAFTTLITYLLITAI
                     QAPSAYFYSATPDPRGQSLSRFTLLLGIAVMAWIAGMWAAQNVKGQWLVLASVVVMLA
                     GYAYTARSIVNIYGERYGFIYRAQIWDERDALIESEKAKGNLLIEVPAIDTGEIHTRD
                     MFGSNGKGWRQFSLNCGARYYGVDGLKVKQ"
     gene            2718..3710
                     /gene="mgrA"
                     /locus_tag="PL000_20840"
     CDS             2718..3710
                     /gene="mgrA"
                     /locus_tag="PL000_20840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HNC91385.1"
                     /translation="MNYLPNESRYQTMRYNRSGRSGLKLPAVSLGLWHNFGGVDTFSN
                     SRAMVLRAFDLGITHLDLANNYGPPPGSAEETFGQIIAKDLRPFRDELIISSKAGYTM
                     WDGPYGDWGSRKYLVSSLDQSLKRMGLEYVDIFYHHRPDPETPLEETMQALDYIVRSG
                     RALYVGISNYPAEKARAAAKMLRELGTPCLIHQPAYSMFNRWVEDGLLATLKEEGVGC
                     IAFSPLAQGLLTNKYLGGGIPEGSRASKAHGFLKPANITDEKLNKVRKLNELAQSREQ
                     TLAQMALAWVLRHDSMTSVLIGASKVEQIDDAIGTLKHLEFTAAELEQIETILK"
     gene            3732..>4307
                     /locus_tag="PL000_20845"
     CDS             3732..>4307
                     /locus_tag="PL000_20845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC91386.1"
                     /translation="MRPKRIILIRHAESEGNLDHTRYQTVQDFALRLSSTGVQQARLA
                     GVQLKEILEDGKVYVYLSPFFRTRETFQLIREAISQNIVKSIEDPRIREQDWGHLRHP
                     DDNKGIIEERDYFSTFYYRIPDGESGADVYDRVSSFLDTLHRDFEKENFPENVLIVSH
                     GLTMRLFLMRWFHWSVEEFESLHNPKNCQIVI"
BASE COUNT         1074 a         1212 c         1008 g         1013 t
ORIGIN      
        1 cccccattct cgtcacaggt gcaagcggat ttgtcgccat tcacaccgtc attcaactgc
       61 ttcagcaggg atataacgtc cgcggtacga ttcgctcact cagcaaggaa gctgaccttc
      121 gcaagaccat ctcaaactac gtacaggcaa atgatcggct ggagattctg tccgctgacc
      181 tgagtcagga tgcgggttgg gatgaagcga tgcaaaacgt cgaaactgtt ttgcatgtcg
      241 cgtctccatt tccattgtac gagccaaaac atgaagatga attgatcgtc cccgccgtgc
      301 aggggacatt acgtgttttg cgtgccgctc acaaggcggg cgtgaagcgt atggtacagg
      361 tgtcatcggt ggccgcgatc tcatcgggac ataatggcga gaacaagacc ttcagcgaag
      421 ccgattggtc tgatctgacg aaagacatcg gtgcgtatgc caaaagtaaa accctcgccg
      481 aacgcgccgc atgggatttt atccacggca gtgaaaatac aaacaaaatg cagctcacca
      541 ccatcaaccc tccgttgatt ctagggccag tgccgaacaa ggatttccgc acttccattg
      601 aattggtccg cacgatcatg ttggggcagg tacctggcgt ggggcgcatc aaaatgggtt
      661 cagtggacgt gcgcgatgta gcctctgcgt tgatccttgc catgcaaacg cccgaagcag
      721 cgggtcaaag gatcctggtt gcagcagacc gttcactttg gctgaaagag atcgcagaac
      781 tcctgcacgg caaatatgcg ggaagatata aaatcaacaa gctggaattt ccaagcatcc
      841 ttgtacgttt gatcgctttg ttcgataaaa aggttgcccg cgtcaccgaa agcctcgatt
      901 gggatcacga actctcgaac gaaaacgcaa aacgcatcct caaatggact ccgcgctctg
      961 cagaagaagg tatattgagc atggccgaaa gtctgatcga acaaggtgtg atataaaatc
     1021 tcttatcatt ttgaatgata atttttgaac cacggagaca ccaagacaca gaggtaaaaa
     1081 agactccgtg actctgtgtc tccgtggtaa agtgttgata ttctcaaaaa ccgccaaatc
     1141 tttatcacgg attaacacat gcagaaaaaa ctcaccaccc ttatcgtcat ccttggcaac
     1201 atcaccgttc tttcaggcct cgccttgctg gcctatctcg gcttctacaa ccgctactgg
     1261 gccgatgact ggtgttacag cgccgatgcc cgtcagcttg gcaccattcc agccacgctg
     1321 caatatttcg acaccgaaga cacgggatat tcctccaacc gctactcgct caccttcttc
     1381 tccgcgctta ccgaaaacac actcggcatg ttcggcaatc aacttgttgc cacactcacc
     1441 atctcactct ggctggtggg cctcgtgtgg acaggctgga atgtttccaa actcatcaaa
     1501 cctctgccct tcgcagccat cctgtttgca tcgtcactgc tgctttttta caacctctat
     1561 ctttcaccgc agcgttttca aatcctttac tggcgctcgg gtgtacttcc ctacagcacc
     1621 gccatcgtgt tctggatgct gatccttggc tttgtcactt cccaaataaa tcgcgaaacg
     1681 cccgcccgct gggtaaatta catcgttgcg ccgcttgcct ttttggcggc aggtctcggt
     1741 gagattagct gcgtatttct tttcagcggc acaacgctat tcctactcgc cgcatggtac
     1801 gcgaaaaagc aaaagcaaca ttgggcagag caatccttcc agaccctttt catcgcatgg
     1861 ctgttcttat tgctcggcat gatcgctctc atcgtctccc cctccaacgc gcgcgtcgca
     1921 gacatgaacg tcaaacgcag cagcctattc agcgtccctt tcgtttcgct gaaacacgcc
     1981 attgacttca tcgttttctc tttgaaagac ctgcccctgc cacatgccat cttcattgga
     2041 atattcctcg gcttcggcat tatcaccaca aaagcagacg attcaacctt aaccgtaaaa
     2101 cgggttgcga ttctcctggc attcaccacc ctcatcactt acctgctcat cacagccatt
     2161 caagcgccga gcgcatattt ctacagcgcc acccccgacc cgcgtggaca gtcgctctcg
     2221 cgcttcaccc tgctgcttgg aatcgccgtc atggcgtgga tcgcaggcat gtgggctgcc
     2281 caaaatgtca aaggtcaatg gcttgtgctg gcatctgttg tggtcatgct tgcaggctat
     2341 gcatacacag cccgaagcat cgtcaacatt tacggtgaac gctacggttt tatctatcgc
     2401 gcccaaattt gggatgagcg ggatgccctg atcgaaagcg aaaaagcaaa aggcaacctg
     2461 ttgatcgaag tcccagccat tgatacgggt gaaattcaca cccgcgacat gttcggttcc
     2521 aacggaaaag gctggcgtca gttctccctc aattgcggcg cccgttacta cggtgtggac
     2581 ggattaaaag tgaagcagta aaaaattttt gtcgttgcga ggacgttctt gttcttgtcc
     2641 gaagcaatct ctaactcaac tggagattgc ttcgtcgcca aagaacggct cctcgcaatg
     2701 acataccagg agaaactatg aactacctac caaacgaatc tcgttatcaa accatgcgct
     2761 acaaccgctc aggccgcagc ggacttaaac tcccagccgt ttcattgggc ttgtggcaca
     2821 acttcggcgg cgtggataca ttttccaaca gccgcgccat ggtcttgcgc gcttttgacc
     2881 tcggcatcac ccatcttgat ctcgccaaca attacggtcc tccccctggc tctgctgaag
     2941 aaaccttcgg tcaaatcatc gccaaggatc tacgtccatt tcgcgatgaa ctgatcatct
     3001 cttccaaggc aggttacacc atgtgggacg gtccctacgg agattggggc tcgcgcaaat
     3061 acctcgtctc cagcctcgat caaagcctca aacgcatggg ccttgaatac gtggacattt
     3121 tctaccatca ccgccccgac cctgaaactc cgctcgaaga gaccatgcag gcgcttgatt
     3181 acattgtccg cagtggacgg gcattgtatg tgggcatctc caattacccc gccgaaaaag
     3241 cccgtgcggc tgcaaaaatg ctgcgcgagc tcggcacgcc ctgcctgatc caccagccag
     3301 cttacagcat gttcaatcgc tgggtggaag atggtctgct tgccacgctg aaagaagaag
     3361 gcgtcggctg cattgccttc tccccgcttg cgcaaggctt gctcaccaat aaatatctcg
     3421 gcggcggaat ccccgaaggc tcacgcgcat cgaaagcaca tggcttcctc aagcctgcca
     3481 acatcaccga cgaaaagctg aataaagtga gaaagctgaa cgaattggcc caatcccgcg
     3541 aacagactct cgcgcaaatg gcgctggcat gggttctgcg ccacgattca atgacctccg
     3601 tgctgatcgg tgccagtaaa gtggaacaga tcgacgatgc aattggaaca ttgaagcatc
     3661 tcgaattcac tgcagcagag cttgagcaga tcgaaaccat tttgaagtaa gcatcaaacg
     3721 ggagttttgg aatgagaccc aaacgaatta tcctgattcg gcacgcggaa tcagaaggaa
     3781 atcttgacca tacacgctat caaaccgttc aggatttcgc cctgcgatta tcctccacgg
     3841 gcgttcagca ggctcggctg gcaggcgtgc aactcaaaga gatcctcgaa gacgggaaag
     3901 tttacgtcta tctctctccc ttcttcagaa cacgcgaaac gtttcaactc attcgagaag
     3961 cgatcagcca gaatatcgtc aaatcaattg aagaccccag aatacgagag caggattggg
     4021 gacacttgcg ccacccagat gacaacaagg gaattatcga agagcgggat tacttcagca
     4081 ctttttatta ccgcatccct gatggtgaat ctggtgcaga tgtgtacgac cgtgtcagca
     4141 gtttcctcga tacccttcac agggatttcg aaaaagaaaa tttccccgag aatgttttga
     4201 tcgtttctca cgggctgacg atgagattat tcctaatgcg ctggtttcat tggagtgtgg
     4261 aggaatttga aagcctgcac aaccccaaga attgtcagat cgtcatc
//
LOCUS       DAOQLC010000233         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9964_length_4299_cov_24.540057, whole genome shotgun sequence.
ACCESSION   DAOQLC010000233 DAOQLC010000000
VERSION     DAOQLC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9964_length_4299_cov_24.540057"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PL000_20850"
     CDS             <1..974
                     /locus_tag="PL000_20850"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HNC91387.1"
                     /translation="KEWDALDVVSKWLIATRSAVTLVTVYSCVIAGILAARDGHFSWV
                     PFLIVTLGLFIAHGTNNILNDYTDFNRGVDRDNYFRTQYGVHPLVQKFWDNSTSIRWF
                     IVSGVLATLSGVYALFYTKFSMTTIGLFAFGAVVLLAYTYPFKYWGLGEFAIFLIWGP
                     IMIAGVYFVLSGIWSWNVVLAGIPFGLSVASINVAKHIDKHDPDKEKGVGTFPVRVGE
                     ANARRVDQLAIVLIYAVIVYLVFVPRYFTPAMLIVLLAYKEALMVIGVLNKPKPAQAP
                     EGWPAWPVWFSGFAFQHNRKFGGLLIMGLIIDALLRAFLPTFWPGLF"
     gene            complement(985..1722)
                     /locus_tag="PL000_20855"
     CDS             complement(985..1722)
                     /locus_tag="PL000_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91388.1"
                     /translation="MNARATQNANVISTEKGWRLGIQKGDSLSYRDAQLDDYSLLPRR
                     KFPHRALTLSLRAKTTGSSLPGTWGFGLWNDPFGLSLGFGGSPFRLPALPNAVWFFYG
                     SQENYLSFSPDTRKTAANGFMAQTFRSPKFHPRLIPAGMALPFSRKATRNLLGKVIDE
                     DSVALSVDVTQWHGYRLEWSAKRSAFWVDDVLVFESPVSPNPRLGVILWIDNQFAAFT
                     PEGKIGFGVLENPEPAWLEIEDIKIVS"
     gene            complement(1754..2641)
                     /locus_tag="PL000_20860"
     CDS             complement(1754..2641)
                     /locus_tag="PL000_20860"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC91389.1"
                     /translation="MQKTKLIPYLEALFAVVVWGASFIATKIAVGQMSPIAVVWLRFA
                     MGIPILFAAVVMRKQFAFPKGMEWLYFALLGFLGISFHQWLQSNGLQTAQATTTAWIV
                     STSPAFIAILGWLILKEKLNLTQSLGIVLAMVGVLAVVSKGDLATIAIGKFGTYGDFL
                     ILISSVNWAVFSILSRRGLKDHPSTRMTFWVMTIGWLITTTAFLAGQNYTEISRLDSR
                     GWMAMIFLGIFTTGLAYIAWFDALSHLPAAQTGAFLFVEPLTSMVVAALILSEEITLV
                     SILGGAVILVGIWLVNRKG"
     gene            2760..4253
                     /locus_tag="PL000_20865"
     CDS             2760..4253
                     /locus_tag="PL000_20865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNC91390.1"
                     /translation="MSSRILLLNPARHFIANEAGVGYLTPLGLVLIGGPLMDAGFEVK
                     LIDHDMNGWPMERLLEEVAAYQPNYILLGHSGSTASHNMVVQTVRELRKAFPEIRIIY
                     GGVYPSYAFESILRQVPEIDVIVRGEGEKTIVELISAWEKGVNLEQVAGIAWRRNGEI
                     VVNRPQPAIQNLDDYRPGWELLDWKHYQMFGFDHAAGLQFSRGCTLTCSYCGQWMFWK
                     KWRHRSPQNIVENMKLLKEKFGVRLIWFADENFAADRDLAKDLLERIIAADLGLSLNL
                     NMTAADVVRDVDLLPLYKKAGVDYIVMGVESLKDSVVAGIRKNNPFATSKEAVRLLRQ
                     HDIISLTNIIYGLEDESWQTLFEKFRGMLELDSDILNAMYLTPHFWTSDGKATDPAKI
                     IQADLAKWSYRNQVIATSYLNPFELFLGVKLTEALFHLRPKALWRLVAHRDGRYRQIM
                     RTSILAGIRVVLAEIWEFAFDTKFVKQGKLTEIIGAERSRQDATPLA"
BASE COUNT          998 a         1112 c         1111 g         1078 t
ORIGIN      
        1 agaaagagtg ggatgccctt gatgtcgttt ccaaatggtt gatcgccacc cgttccgctg
       61 tgacccttgt caccgtttat tcctgcgtta ttgcaggtat cctcgccgcc cgcgatggac
      121 acttctcatg ggttccgttc ctcatcgtca cccttggcct gttcattgcc cacggcacca
      181 acaacatcct caacgattac actgacttca atcgcggcgt tgatcgggat aactacttcc
      241 gcacccagta cggtgtccat cccctcgttc aaaaattctg ggataactcc acctccatcc
      301 gctggtttat cgtcagcggt gtgttggcca ccctttcagg tgtctacgcc ctgttctata
      361 ccaaattctc aatgaccacc atcggactgt tcgcctttgg cgcggtcgtt ctgctggcct
      421 acacgtatcc cttcaaatat tggggcctcg gcgaattcgc catcttcctc atctggggac
      481 cgatcatgat cgcgggtgtg tactttgtgc tttcaggcat ctggagttgg aacgtcgtcc
      541 ttgcgggcat tccgttcggg ttgagcgttg ccagcatcaa cgttgccaag cacattgaca
      601 agcatgaccc cgacaaggaa aaaggcgtcg gcacattccc cgtccgtgtg ggggaggcca
      661 acgcccgccg tgtggatcag cttgccattg tgttgatcta tgctgttatc gtctaccttg
      721 tgtttgttcc gcgctatttc acccctgcca tgctcattgt tctccttgca tacaaggaag
      781 ccctcatggt catcggcgta ctgaacaagc ccaagcccgc ccaggctcct gaaggctggc
      841 ctgcctggcc ggtgtggttc tctggttttg ccttccagca caaccgcaaa ttcggcggcc
      901 tgctcatcat gggcctcatc attgacgcgc tgttgcgcgc cttcctgccc accttctggc
      961 ccgggctgtt ctaaacagaa atattcacga aacgatcttt atatcttcaa tttccaacca
     1021 tgcgggttcg gggttttcca aaactccgaa cccgattttt ccttcagggg tgaatgccgc
     1081 aaattgattg tcgatccaga ggatgactcc cagccgcgga ttcggactca cgggcgactc
     1141 aaacacgaga acatcatcca cccaaaaagc agaccgcttc gcgctccact cgagcctgta
     1201 tccatgccat tgagtgacat ccacgctgag agcgaccgaa tcctcatcaa tcacttttcc
     1261 taataaatta cgagtcgcct tgcgcgagaa cggcagcgcc atccccgcag ggatcaaccg
     1321 cggatgaaat ttcggcgagc gaaaggtctg cgccatgaat ccgtttgccg ctgtttttcg
     1381 ggtatcaggg ctaaacgata aataattttc ctgcgaccca taaaagaacc aaaccgcatt
     1441 cggcaatgcg ggcaatcgga acggacttcc cccaaacccc agtgacaacc caaacgggtc
     1501 gttccacagc ccaaagcccc atgttccggg gagggaactg cctgtagttt ttgcacgcaa
     1561 actcaaagtt agagcgcgat gcggaaactt ccttctcggc agcagggaat agtcatcgag
     1621 ttgggcgtct cgatagctaa gagaatcccc tttctgtatt cccagccgcc accccttttc
     1681 agttgagatt acgtttgcat tctgagttgc ccttgcattc atccttcatc cttcataatt
     1741 catccttatc ttttcatcct tttctattta ccagccaaat gcccaccaga atcaccgcgc
     1801 cgcctaaaat ggatacgagc gtgatctctt cgctcaaaat gagtgcagcc accaccatgc
     1861 tggtgagcgg ctcaacaaaa aggaacgcgc ctgtttgtgc agcgggcagg tggctgagcg
     1921 catcgaacca ggcaatgtag gccagccctg tggtgaagat gccgagaaag atcattgcca
     1981 tccagccgcg cgaatcgagg cgggaaattt cggtgtagtt ttggcccgct aaaaatgccg
     2041 tagtggtgat cagccagccg atggtcatta cccaaaatgt catccgtgtg gaggggtggt
     2101 ctttcagacc gcggcgtgac aaaatggaga acactgccca attgactgag ctgatgagga
     2161 tgagaaaatc accataggtg ccgaatttgc cgatggcaat ggtggcaagg tctcctttgc
     2221 ttaccactgc cagcacacca accatggcca gcacaatgcc cagtgattgt gtcaggttga
     2281 gtttttcctt caagatcagc cagccgagaa tggcgatgaa tgcaggcgat gtggaaacga
     2341 tccacgcggt ggtggtggct tgggctgttt gcagtccgtt tgattgcagc cattgatgaa
     2401 atgagattcc caaaaagccg agcagggcaa aatagagcca ttccattcct ttgggaaagg
     2461 cgaattgttt gcgcataacc actgcggcaa acaggatcgg gatgcccatc gcaaatcgca
     2521 gccacactac agcaatggga gacatctgcc cgacggcgat ttttgtggca atgaaagacg
     2581 cgccccaaac aacaacagcg aacagggctt caagataggg gataagtttt gttttttgca
     2641 tgagggttcc tctaggaaat ttggctgatt ataaatctct tgacacgagt tttgctggtg
     2701 ataaaattca caaaaagaaa gatgtttatg gtctttttta tctgatttat ggaggcaata
     2761 tgtcatcacg aattctcttg ttaaatcctg cacgtcattt cattgcaaat gaggctgggg
     2821 ttgggtacct gacgccattg gggttggtat tgatcggcgg gccattgatg gatgctggtt
     2881 ttgaagtaaa actgatcgac cacgacatga acggctggcc catggagcgg ttgttggagg
     2941 aggtggccgc ataccagcca aactatattt tgcttggaca ttcaggttcc acagcttcgc
     3001 acaacatggt tgtgcagacc gtgagggagc ttcgcaaggc cttcccagag attcgaatca
     3061 tttatggggg tgtttatcca tcctatgcct ttgagagcat tcttcggcag gttccagaga
     3121 tcgatgtcat tgtccgcggt gaaggggaga aaacaatcgt tgagcttatt tcagcttggg
     3181 aaaaaggcgt aaacctggaa caggttgcgg gaattgcctg gcgcagaaat ggtgagatcg
     3241 tggtcaatcg tccgcagcct gcgattcaaa acctggacga ttaccgccct ggctgggaac
     3301 tgctggattg gaaacactat caaatgttcg gctttgacca tgccgcaggt ctgcaattta
     3361 gccgcggttg tacactcacc tgcagttatt gcggtcagtg gatgttttgg aagaagtggc
     3421 ggcatcgcag tcctcagaat attgtggaga acatgaaact gctcaaggaa aaattcggtg
     3481 tgaggctgat ctggtttgcc gatgagaatt ttgcagccga ccgtgaccta gcaaaggatc
     3541 tgcttgaacg catcatcgcc gctgaccttg gcctctcgct caacttgaac atgaccgccg
     3601 ctgacgttgt ccgcgatgtg gacctgcttc cgctttataa aaaggcaggt gtggactaca
     3661 ttgtgatggg cgtggagtcg ctcaaggata gtgttgtggc tggcatccgc aagaacaatc
     3721 ccttcgcgac ttcaaaagaa gcggtgcggt tattgcgcca gcacgacatc atcagcctga
     3781 caaacatcat ctatggcctt gaagatgaat cgtggcagac cctctttgag aaattcaggg
     3841 gcatgcttga gttggattcc gatattttga acgccatgta cctgaccccg cacttttgga
     3901 catcggatgg gaaagccacg gatccagcga agatcattca agccgacctt gcaaaatggt
     3961 cgtatcgaaa tcaggtcatt gccacttcgt acttgaatcc atttgaattg tttcttgggg
     4021 tgaaactgac cgaggctttg ttccatctcc gcccgaaggc gttgtggcgg ttggttgcac
     4081 accgagatgg acgctatcgc cagatcatgc ggacttcgat cctggcgggc atccgtgtgg
     4141 tgttggcgga aatttgggaa tttgccttcg atacgaaatt tgtaaaacag gggaagctga
     4201 cagagataat cggcgcggaa cgttcaaggc aggatgccac tccgctggct tgaagatcag
     4261 ttcttgaaat tttccagcag tttctggtaa agggattcg
//
LOCUS       DAOQLC010000234         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_9988_length_4294_cov_26.316112, whole genome shotgun sequence.
ACCESSION   DAOQLC010000234 DAOQLC010000000
VERSION     DAOQLC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9988_length_4294_cov_26.316112"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PL000_20870"
     CDS             complement(<1..653)
                     /locus_tag="PL000_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNC91391.1"
                     /translation="MEIKFGTHTIGLNHPTYFIADIAANHDGDMERAKKLIRLAKEAG
                     ADAAKFQNFDAPKIVSDYGFKAMSGGQVSHQAAWKKSVFEVYKGASIPFEWSMTLVEE
                     CKEVGIDYFSSPYDFEAIDFLDQYVEVYKAGSGEIDWIEALERMAGKGKPFFIATGAS
                     TIGEVQKAVHAILKLNKQLVLMQCNTNYTANPNNYDHLHLNVLKTYAAMFPDVILGLS
                     "
     gene            complement(691..1257)
                     /locus_tag="PL000_20875"
     CDS             complement(691..1257)
                     /locus_tag="PL000_20875"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC91392.1"
                     /translation="MTDLNPKQNPDQHLTPQQLAREVVYQSRWVNLYKDTVKFPNGFV
                     IDKFHLLDFEHEAVTAIMENTAGEVIFVLIPRYATGTVEWELPAGGMEIGETAIEAAR
                     RELLEETGYSSEGHVLVYSYYPMNGNANQVFHIVHCKVTELVQGFDPNEVSDVHWFTR
                     KEIKQMIKEKKITDGFTLTALLLWLSED"
     gene            1401..1577
                     /locus_tag="PL000_20880"
     CDS             1401..1577
                     /locus_tag="PL000_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91393.1"
                     /translation="MENQWVSLWITLLILWITGRNGSDFVEKWRILCGKMVIFMSKPV
                     DNPGKAVENLLKTY"
     gene            complement(1571..2482)
                     /locus_tag="PL000_20885"
     CDS             complement(1571..2482)
                     /locus_tag="PL000_20885"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC91394.1"
                     /translation="MKLLITGASGLLGINLAQETMHTHEIVGVDRGKLAHAPFPILKT
                     DLLAPGAVDSIIETARPDWVINCAALANLEECEENPDLARRLNTDMPAQLAKACKSRG
                     TAFIHISTDAVFDGTSLSFYKEEDEPNPLSLYAKTKLEGERAVLAEDPNAIVTRVNFY
                     GWSLTGRRSLAEFFFHNLTYNKSMSGFTDVKFCPMLVNHTARTLVKMLARGLHGLYHL
                     VGPQAMSKYQFGVEIARKFGLREGEISPKSINTSSLNARRSHNLCLSVNKISTDLGEA
                     LPEFSTGLNEFYTQYQQDYPQKIRSYQ"
     assembly_gap    2507..2606
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2607..3598)
                     /gene="pseB"
                     /locus_tag="PL000_20890"
     CDS             complement(<2607..3598)
                     /gene="pseB"
                     /locus_tag="PL000_20890"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNC91395.1"
                     /translation="MDWKNQVVLVTGGTGSFGKKFTKILLEEKQPKKVIIFSRDELKQ
                     HEMQVGGFNDPRLRYFIGDVRDRERLVRAMHGVDIVVHAAALKQVPACEYNPMEAIKT
                     NIMGTANVVEAALDAGVKKVLTVSTDKAVSPANLYGATKLAAEKLTIQSNAYAGGSAT
                     RFSCVRYGNVVGSRGSVVPLFLKQRSGGKITITDDRMTRFWLSLEQGVRFVIDCIEQM
                     EGGEVFVPKIPSTKVVDLARAIAPDAEINIIGIRPGEKLHEMLISEDEARHTIELEKM
                     YVVQPAEATWFGYSWQDKGKSLNEGTYYSSDNNSEWLDIEGIKKYIAPFEELFA"
     gene            complement(3683..>4294)
                     /locus_tag="PL000_20895"
     CDS             complement(3683..>4294)
                     /locus_tag="PL000_20895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC91396.1"
                     /translation="GTMWNQCADRIPAGWTITLSDLSDGMLDSAWRNLVVTGRSFKFE
                     KIDAQSIPYADGTFDAVIANHMLYHVPDRKKALTEIKRVLKGDGCLIASTVGANHMKE
                     MYGWLQRVNKNPRPDMFTNPFTLESGLAQLLEVFPNVEKSQYVDNLRVTELDPLMNYI
                     RSSIPAEDISEPEFETLKKEFAAVLKKDGEIFISKDSGLFKAL"
BASE COUNT          927 a          999 c         1145 g         1123 t
ORIGIN      
        1 gagagtccca gaatcacatc ggggaacatg gctgcatagg ttttcagcac gttcaagtgc
       61 aaatggtcgt aattgttcgg gttggcggtg taattggtgt tgcactgcat cagcacgagt
      121 tgtttgttca atttcagaat cgcatggaca gccttttgaa cttcgccaat ggtggatgct
      181 cccgtggcaa tgaagaaggg cttgcctttc ccagccatgc gttcgagcgc ttcgatccag
      241 tctatttcac cggagccagc cttgtacact tccacatact gatcgaggaa atcaatggct
      301 tcgaaatcgt agggcgaaga gaagtagtca atgccgactt ctttgcattc ttcaaccaga
      361 gtcatcgacc attcaaatgg aatggatgcg cccttgtaca cttcaaaaac ggatttcttc
      421 cacgcagcct gatggctgac ctgtccgccg ctcatggctt tgaaaccgta atctgaaacg
      481 atcttgggcg cgtcgaaatt ctggaatttg gcggcatcgg cgcctgcttc cttggcgagg
      541 cggatgagtt ttttggcacg ctccatgtca ccgtcgtgat tggcggcaat atctgcaatg
      601 aagtaggtgg gatggttcaa gccgatggtg tgggttccaa atttgatttc cattttttgt
      661 tcctccgggt ggatggtttt tctttttatt tcagtcctcg ctcagccaga gcaataacgc
      721 agtgagggta aatccgtctg taatcttctt ttcctttatc atttgcttga tctctttccg
      781 cgtaaaccag tggacatcac tgacttcgtt cgggtcgaaa ccctgcacca gttccgtaac
      841 cttgcaatgc acaatgtgaa agacctgatt ggcatttccg ttcatcgggt aataggaata
      901 taccaagaca tgcccctcgg aagaataccc ggtctcttcg agcaattccc tgcgggcggc
      961 ttcaatggcg gtttcgccga tctccattcc gcctgcaggc agttcccatt caaccgtgcc
     1021 ggtggcatag cgcgggatga ggacaaagat aacctcacct gctgtatttt ccatgatcgc
     1081 agtaacagct tcgtgctcaa aatccagcag gtggaattta tcgatcacaa agccgtttgg
     1141 gaatttgaca gtgtctttgt acaggttcac ccacctgctt tgatacacca cctctctggc
     1201 cagttgttgc ggcgttaaat gttggtctgg gttttgtttg gggtttaggt cagtcatatt
     1261 gtctcggatg ccgttaagtt ttttgtggag aagcaggttt cgggtaatgg tgaaacgcgt
     1321 ttttcgggtg gattccgtta agatggcagg gtcaatgtta cgggtggtgt gggtacttcg
     1381 gggaaaagtc ctcaaaaaaa gtggaaaacc agtgggtttc cctgtggata actctgttga
     1441 ttctgtggat aactggcagg aatggctccg attttgtgga aaaatggcgg attctctgtg
     1501 gaaaaatggt catttttatg tcaaagcctg tggataaccc ggggaaagca gtggaaaacc
     1561 tgctcaaaac ctactgataa ctgcggattt tctgtggata atcctgctga tattgtgtgt
     1621 aaaactcgtt caggcctgtg gaaaactcgg gtaaagcctc gcccaaatct gtggatatct
     1681 tgttgacaga caggcataag ttgtgggaac ggcgggcatt aaggctggaa gtgttgatgg
     1741 atttggggga gatttcgcct tcacgcaggc cgaacttgcg ggcgatctcg accccaaact
     1801 ggtatttgct catggcctgc ggaccaacga ggtgatacaa gccatgcagt cccctggcaa
     1861 gcattttgac gagggtgcgg gcagtatggt tgacaagcat gggacagaat ttcacgtcgg
     1921 taaagccgct catgcttttg ttgtaggtca ggttgtggaa aaagaattcg gcgagactgc
     1981 gtcggccggt gaggctccac ccataaaaat tgactcgtgt aacgatggcg ttggggtctt
     2041 ctgcgaggac ggctctttcg ccttccagtt tggttttggc gtacagactc agtggattgg
     2101 gttcgtcttc ttctttgtag aaggaaagcg aggttccatc gaagaccgca tcagtggaaa
     2161 tgtggataaa agcagtgccg cgcgacttgc aggctttggc cagttgggca ggcatatcgg
     2221 tgttgaggcg gcgggcaagg tcgggatttt cttcgcactc ttcgaggttg gcgagtgcgg
     2281 cgcaattgat gacccaatcg gggcgggcgg tttcgatgat ggaatccacc gcaccggggg
     2341 caagcaggtc ggttttcaag atcgggaatg gggcatgagc caacttgccg cgatccacac
     2401 ctacgatctc atgggtatgc atggtttcct gcgcgaggtt aatgccaagt aatccgcttg
     2461 caccggtgat gaggagtttc atgtgtcggg ttccaggttt taggttnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnntggg caaacaactc ctcaaatggg gcgatgtact
     2641 ttttaatgcc ttcaatatca agccattccg aattattgtc gctggagtaa taggtgcctt
     2701 cgtttagtga cttgccctta tcctgccacg aataaccgaa ccacgtagct tcggcaggct
     2761 gaaccacata catcttttcg agttcgatgg tgtgacgggc ctcgtcttca gagatgagca
     2821 tctcgtgtaa tttctcgccg gggcgaatgc cgatgatatt gatctcggca tcgggagcga
     2881 tggcacgggc aaggtccacg acctttgtgc tggggatctt cggcacaaag acttcgccgc
     2941 cttccatttg ttcgatgcaa tcaatgacga aacggacacc ctgttcgagc gaaagccaaa
     3001 agcgggtcat gcggtcatct gtgatggtga tcttgccgcc gctgcgttgt ttgaggaata
     3061 gcggaacgac cgaaccgcgt gaaccgacga cattcccgta tcggacgcac gaaaatctgg
     3121 ttgctgaacc cccggcatag gcgttgcttt gaatcgtcag tttttcggcg gccaacttgg
     3181 tggccccata caggttcgca gggctgaccg ccttgtcggt gctgaccgtc aacacttttt
     3241 tgacgcccgc atccagcgcg gcttcgacca cgttggcagt gcccatgatg ttggtcttga
     3301 tggcttccat cgggttgtac tcgcaggcag gcacctgctt gagcgccgcc gcatggacaa
     3361 caatatccac cccatgcatg gcacggacga gtcgctcgcg gtcacgcaca tcaccgatga
     3421 aatagcgcag acgcgggtcg ttgaatccgc ctacctgcat ttcgtgctgc ttcaattcat
     3481 cgcggctgaa gatgatgacc tttttcggtt gtttctcttc gagcaggatc ttcgtaaatt
     3541 ttttaccgaa cgagcctgtt ccaccagtga cgagtacaac ttgatttttc cagtccatga
     3601 tttctccttg atatcggtag ggcaggttga caacccgcca gtgtttatat ttttcaatta
     3661 catgccaggc tgaaagcctg acctacaagg ctttgaacaa gccagagtct ttcgaaataa
     3721 aaatctcacc gtcttttttc agcacggcgg cgaattcttt tttaagcgtt tcaaattcag
     3781 gttcggaaat atcctcagca gggatggagg aacggatgta gttcatcaac gggtcaagtt
     3841 ctgtgacacg caggttatca acatattgcg atttttccac atttggaaaa acctccagca
     3901 attgggcaag tccgctttcg agggtaaatg ggttggtgaa catatcgggg cgcggatttt
     3961 tgttcacacg ctgcagccag ccgtacattt ccttcatgtg attcgccccg accgtggaag
     4021 cgatcaaaca gccgtcgccc ttgagcacgc gcttgatctc tgtcaaagcc tttttacggt
     4081 cagggacgtg atagagcatg tgattggcga tgaccgcatc gaaggttcca tcggcataag
     4141 gaatggattg cgcatcgatc ttttcgaact tgaagctgcg tcccgttacg accagattcc
     4201 gccacgctga atcaagcatc ccgtcggaaa gatcggagag ggtgatggtc cagcctgcgg
     4261 ggattcgatc cgcgcattga ttccacatcg tgcc
//
LOCUS       DAOQLC010000235         4279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10040_length_4279_cov_19.627604, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000235 DAOQLC010000000
VERSION     DAOQLC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_4279_cov_19.627604"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..328
                     /locus_tag="PL000_20900"
     CDS             32..328
                     /locus_tag="PL000_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91397.1"
                     /translation="MKTLFRILVIMVVAVLIGGAFYSAVTASSSGTDGTSIQERPLPD
                     GFPAERPEGEDFEGGIQFPAEMIKSLVIMTVVATASLNLPKLFNWKKTNLKAGL"
     gene            510..1028
                     /locus_tag="PL000_20905"
     CDS             510..1028
                     /locus_tag="PL000_20905"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNC91398.1"
                     /translation="MTTEVVKNTPVQTKTKLWLDVFLFVAFLITMEPHPSGLAIHEWL
                     SLAMVLTMIVHLLLSWDWITEITRRFLGKLGMQNRINYILNWLLFFDGTLLIVSGVMI
                     SEVALPAMGIQLPERFAWRRLHDLSANLGLLMLGIHTALHWNWVVNTVNRYILKPVLG
                     LFSPKQKKDAAI"
     gene            1025..1390
                     /locus_tag="PL000_20910"
     CDS             1025..1390
                     /locus_tag="PL000_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91399.1"
                     /translation="MKTLGRTLAILAVFSVTVFLLVTSVNTLGNLIDSDGPRFEQGSP
                     EFRPPEGGENFRPEGGDGDREGGSRGFRINNPRRLATGMVKDTIVVGVLVTAIALPKS
                     FFKKKKRTPKPTATSGGVG"
     gene            complement(1467..2816)
                     /locus_tag="PL000_20915"
     CDS             complement(1467..2816)
                     /locus_tag="PL000_20915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91400.1"
                     /translation="MKLTSKLILAFLVVSLFSTGIIVVITRVATNREFDKFISDQYKA
                     DLVEQLARHYQKYQTWDGLETNFNPFDNDHFRPDDGRPAFFSIADTDGRILVAGAGYR
                     PEEIVSAEKLNKGTPIQVENETVGILLVNTSPEKNPMEDEFIHRLNDSIFLSAVITII
                     LALILGAILSRTITRPIRELTKATHEMADGKLGQQVPVRSRDEIGELASSFNKMNGDL
                     ARSLNLRKQMTADIAHELRTPLSLILGHAEGVHDGVLPPSHENFEIIREEAERLEKLV
                     NDLRTLSLADAGELSVDFQPVDINELLNDVKTHYMTLFNQKRITLNLKPDSATLRVKV
                     NLDPIRFSQVLTNILDNALRYTPENGQVTMATTKVENQIEVSVQDDGEGVPPEDAAHL
                     FDRFYRADPSRTRDAGGSGLGLAIAKSIIEMHQGKIWAESEKGKGMKIIIRLPLSRN"
     gene            complement(2819..3502)
                     /locus_tag="PL000_20920"
     CDS             complement(2819..3502)
                     /locus_tag="PL000_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91401.1"
                     /translation="MPQTILIVDDEKRLVSLVQSYLMQEGYRVVTAYNGKEALPIAQK
                     EKPDLIILDIMMPEMNGYDFMRTHRTEADTPIIMLTAKVEDDDKVIGLELGADDYVTK
                     PFKPRELMARVRNVLRRTGKNEAKAKTLRAGEITVNRDTREVLVVDRSIDLTPSEFDL
                     LTALMSSPGRVFSRLDLLDVIQGVRYEGYERTIDTHVKNLRAKIENDPRKPRYIETVY
                     GVGYRLNKG"
     gene            complement(3668..4135)
                     /locus_tag="PL000_20925"
     CDS             complement(3668..4135)
                     /locus_tag="PL000_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91402.1"
                     /translation="MKNRKWLTYTLGTLLTLIVLAAVGGVGFRIGMMQNVSAMTGRPA
                     FAHNFDGDFQPPMQGNHPNDGNSKNGQMNPHQDNFRGNDRGGGPRFFSPIFGLIRLAA
                     LAALLWLGYKFVQKSGWKLTRVTQAQAATETPSTPVETESAAPVNSDENKDEA"
BASE COUNT          965 a         1042 c         1151 g         1121 t
ORIGIN      
        1 gccatgtcat tgcacagaaa ggatgcctaa aatgaagact ctgttcagaa ttctcgtgat
       61 catggttgtg gctgtgttga tcggcggcgc tttctacagt gcagtgaccg cttcctcctc
      121 agggacagat ggcacatcca ttcaggaacg tccactcccc gatggtttcc ccgcagaacg
      181 gccagaaggt gaagatttcg aaggcggaat ccaattccca gccgaaatga tcaaaagcct
      241 ggtgatcatg accgtcgttg caaccgcctc actgaacctt ccaaaacttt tcaactggaa
      301 aaaaaccaac ttgaaagcag gcctgtaacc ctttttcgca cattccccga ccaaccagtt
      361 ggggaatgtg tttacaactt cttcacaatt aatccgcaaa acccccatca caaaatataa
      421 taagctcata aaattgggca gttcacaaga ccgcctacaa acccataaga cactgctcgc
      481 agggttggca atgaatttca aggagaaaca tgaccacaga agttgtaaaa aatacaccag
      541 tccaaaccaa aaccaagttg tggctggatg tctttctttt tgtcgcgttt ttgatcacga
      601 tggagccgca cccaagcggg cttgccattc atgaatggtt gagcctcgcc atggtgttga
      661 cgatgatcgt ccacctgctg ttgagttggg attggatcac ggagatcaca cgccgttttt
      721 tgggaaaact cgggatgcag aaccgcatca actacatctt gaactggctg ctgttcttcg
      781 acgggactct gctcatcgta tctggtgtga tgatctcgga agtggccctg cctgcgatgg
      841 gcattcagct ccccgaaaga tttgcctggc gcagactgca tgacttgtcg gccaaccttg
      901 gcctgctcat gctgggaatt cacaccgccc tgcattggaa ttgggttgtc aatacggtaa
      961 accgctatat actcaagcct gttctgggct tgttttctcc gaagcaaaag aaggatgccg
     1021 caatatgaaa acgctcggac gcacccttgc cattctggct gtgttttctg taacggtgtt
     1081 cctgctcgtg acctccgtca atacacttgg gaacttgatc gactcagatg gtccacgttt
     1141 tgaacaaggc agccccgaat tcaggcctcc tgaaggcggc gaaaactttc gacccgaagg
     1201 cggcgatgga gaccgtgaag gcggttcgcg tggattcagg atcaacaatc cgcgccgatt
     1261 ggcaacaggc atggtgaaag atacgatcgt ggtgggcgtt ttagtcaccg ccattgccct
     1321 gccaaaaagt tttttcaaaa agaaaaagag aacccccaag ccaactgcca caagcggtgg
     1381 agtgggctaa gcatacgcaa agacgtccgt gaaatgaaaa tttcacggac gtcttttttt
     1441 gtatttccac actgtaagtt gggcgctcaa ttccttgata atggcaggcg gatgatgatc
     1501 ttcatccctt tgcctttttc gctctctgcc cagattttgc cctgatgcat ttcgatgatg
     1561 gatttggcaa tggcaagtcc cagccccgag ccgcctgcat cgcgggtgcg cgaggggtcg
     1621 gcgcggtaga aacggtcaaa gaggtgggcg gcatcttcag ggggcacacc ctctccatcg
     1681 tcttgaacgg aaacttcgat ctgattctca accttggttg ttgccattgt gacttgtccg
     1741 ttttctgggg tgtaacgcag ggcattatcc aggatgttgg tcagtacttg agagaagcgg
     1801 atcgggtcaa gattcacttt cacgcgaagc gttgccgaat caggcttcag gttaagagtg
     1861 atccgctttt ggttgaacag ggtcatgtaa tgggttttga catcgttcaa tagttcgttg
     1921 atgtccacgg gttggaaatc caccgaaagc tcgcctgcat ctgcaaggga aagtgtacgc
     1981 aggtcgttga caagtttttc cagccgttcg gcttcttcgc ggatgatctc aaagttttca
     2041 tgcgagggcg gcagcacacc atcatggacg ccttctgcat ggccgagaat caaactgagg
     2101 ggagtgcgta gttcgtgcgc aatgtcggcg gtcatttgtt tgcgcaggtt gagcgaacgc
     2161 gcgaggtcgc cattcatttt gttgaaagag gatgcgagtt cgccgatctc atcgcgtgag
     2221 cggacaggca cttgttgccc cagtttgcca tcggccattt catgagtggc tttggtcaat
     2281 tcacggatgg ggcgtgtgat ggtgcgcgaa aggatcgccc caagaatcaa agcgaggatg
     2341 atggtaatga ccgcgctgag aaaaatggaa tcgttgaggc gatgaatgaa ttcatcttcc
     2401 atcggattct tttctggcga agtgtttacc agcaggatgc ccaccgtttc attctcaact
     2461 tgaattgggg tgcccttgtt gagtttttcg gcgctgacaa tttcttcggg gcggtatccc
     2521 gcgcctgcaa ctagaatcct gccgtctgta tctgcgatgg agaagaaggc ggggcggccg
     2581 tcgtcaggtc tgaagtggtc gttgtcgaac gggttgaaat ttgtctccag cccatcccag
     2641 gtttggtatt tttgataatg acgggcaagt tgttctacca gatctgcttt gtactggtcg
     2701 ctgatgaatt tgtcgaactc gcggtttgtg gcaacgcgtg tgatcacaac aataatgcct
     2761 gtactgaaca ggcttacaac caaaaacgcg aggatcaatt tggaggtcag tttcatgcct
     2821 agcctttatt caggcggtag ccgacgccat acacggtttc gatatagcgc ggtttgcgcg
     2881 ggtcattttc gatcttggcg cgcaggtttt tgacgtgcgt atcaatggtg cgctcgtagc
     2941 cttcataacg aacaccctgg atcacatcca acaggtcgag gcgagagaac acacgccccg
     3001 gagatgacat gagcgccgtg agcaggtcaa actcggatgg cgtcaggtca atgctgcggt
     3061 ccaccaccag cacctcgcgg gtatctcggt tgactgtgat ctcgcctgcc cgcagggtct
     3121 tggccttggc ttcgtttttc cctgtgcggc gcagcacatt gcgcacacgt gccatgagtt
     3181 cacgcggctt gaagggcttg gtcacataat cgtccgcgcc cagttccagg ccgatcacct
     3241 tgtcatcgtc ttccactttg gcggtcaaca tgatgatggg agtgtcggct tcggtgcggt
     3301 gggtgcgcat aaaatcgtac ccgttcattt cgggcatcat gatgtccaaa atgatgagat
     3361 cgggtttttc tttttgagca atgggcaatg cctctttgcc gttatacgcc gtgacgacac
     3421 gatacccctc ttgcatcagg taactttgta caagggaaac aaggcgtttt tcatcgtcaa
     3481 cgatcaggat ggtttgtggc atgggcattc tctctgttta atggcctgta tttgaaagtt
     3541 tgttggtcgt attatctcat aaggcatggg gatgtagctg tgaagaaatt atgtgctttt
     3601 gtggagaaaa cacccgcctc gatagataga ccgaagcggg tgtttgattc tgttcaaaag
     3661 gctgactcta agcctcgtcc ttattctcgt cactgttgac tggggctgcg ctttcggttt
     3721 caacgggtgt ggaaggcgtt tctgtggctg cctgtgcttg tgttacacgg gtcagcttcc
     3781 agccgctctt ctgcacaaac ttgtacccga gccagagcag ggccgccagc gccgccaaac
     3841 ggatcagtcc gaagatgggc gaaaaaaagc gcggaccgcc gccacggtca ttgccgcgga
     3901 agttatcctg gtgcggattc atttgtccgt ttttggaatt gccgtcattt gggtgatttc
     3961 cctgcatcgg cggctggaaa tcgccatcaa agttgtgagc aaatgcgggg cgtcccgtca
     4021 tggctgagac gttttgcatc atcccgatgc ggaagcccac gccgcccacg gcagcaagga
     4081 cgatcaaggt taacaaggtt cccagagtat aggtcaacca ttttctgttt ttcattcttt
     4141 ttctccagta ataatttttt ttgtgtggcc ctgcctgtat gaacagggga taaagcgaat
     4201 gctttattga atcttatttt cctactggag aattacataa gggtggagga attgtgtgga
     4261 acttgtgtgt tcaaaattg
//
LOCUS       DAOQLC010000236         4209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10322_length_4209_cov_20.802841, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000236 DAOQLC010000000
VERSION     DAOQLC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10322_length_4209_cov_20.802841"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PL000_20930"
     CDS             <1..309
                     /locus_tag="PL000_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91403.1"
                     /translation="SPLLYCPANQLPRVEASIFGLRMKYGVNYTPPAASGALFADFPP
                     SDPSYWGIDWAEQAYLDGLLPACGTDSATGKPMFCPSQLVDRGWGAYLIVKAKNLPLP
                     "
     gene            386..1105
                     /locus_tag="PL000_20935"
     CDS             386..1105
                     /locus_tag="PL000_20935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91404.1"
                     /translation="MNQRQLTLLLVDDDAMIVESLGPLLERSGFHVITASTGEEALTK
                     VQAHHPDVIVMDVLMPRMNGREALRRMRKSNIWIPTILLTQVGEASERAVALEEGADD
                     YLNKPFHSQELLARIHAVLRRARPAGQSLSSAHRLISLDLLLDRQSRRAFLADESLNL
                     TPKAFAVLEYLMTHPDEAVSREHLLHSVWGWEYPTGTRTVDTRVNELRRALKDEAGAP
                     RFIQTLTGEGYIFIAPVKPES"
     gene            1102..2025
                     /locus_tag="PL000_20940"
     CDS             1102..2025
                     /locus_tag="PL000_20940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC91405.1"
                     /translation="MSRYKFILPFFPLLLGILSLIFLPSLIKPEVVLSFRLGLGAVLF
                     GLGLLITLWMQSLGVVDSLREKKMRTEMEALTRSYEDSHRRFIRRLDHELKNPLMGLQ
                     ASLENLQAVTELEERRKAEGNIQKALERLVRLLRDLRKLSDLDESKLERESVDIPELV
                     EEIIEVLRSTPGREHRRINLMVTRVPTPPPPVSGDRDLLGISLYNLLDNALKFTVQNE
                     AVEVRVRDDERVVFVEVADGGSGIRPDEQHKVFEELYRGENAHAVEGSGLGLSFVKRI
                     VALHHGELTLRSQRDEQHGSIFTIRLPVTKL"
     gene            2132..>4209
                     /locus_tag="PL000_20945"
     CDS             2132..>4209
                     /locus_tag="PL000_20945"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNC91406.1"
                     /translation="MNNITAKHILYSLLLAGVALLGVYFLPTGNTFQITVETPTSIAS
                     SPMITGVSGDFLWAKRMGGSGTGDFGQGYDIATDLDGNLYFTGGFTGELDFDPGEGTF
                     TLASASLDYSDIFAAKLDSEGNFLWARHIGGQAWDYAYDIAVDSNRDVYVMGSFEGTA
                     DFDPGPDILNLSSAGLRDIFVVRLDENGNLIWAKRMGGASDDGGSAVAVDHDGNVYLT
                     GYFQGTADFDPGENTVNLKSAGKNDIVVTKLDADGNLIWAKRMGGIQNDLGFGLALGA
                     DSVVYTTGRFEGAVDFDPGAETTNFVSIGGSDAFLSKLDADGNFVWAGVVGGNGEAQG
                     FGLVVDSGGSVYLTGDFWRKVDFDPGEKSFDLTSFGDSDVFLCKLDENGNFVWAKQMG
                     GPGAGHGYDLALDPSGNIYTVGSFSDTADFDPGAGEFHLAGNGRYEDIFIAQLDADGN
                     FIWAKSAGGAGDDLGVGIAVDPRGDVVATGNFAGKVDFDPGPEPFYLASQGYYDVFVS
                     KLNGNGIPIFADVGDDHPQFKYIQALYASGLTSGCEVNPLKFCPDAVLNRAQTAVFML
                     RGQFGPAYTPPPAPWDVFSTEDWSGFEWARPWAEGMYAAELTKGCQADPLKFCPTAQL
                     TRMEASIFGLKMKYGQSYLPPPATGDLFADLPAIGPNEWGIAWAEQAYRSGLLPSCGI
                     QNGKPMFCPD"
BASE COUNT          894 a         1069 c         1194 g         1052 t
ORIGIN      
        1 agcccattat tgtattgccc cgccaaccaa cttccgcgtg tggaggccag catctttggt
       61 ctgcgcatga agtatggcgt gaattacact ccgcccgctg caagtggagc tttgttcgca
      121 gacttcccgc ccagtgaccc gagctactgg ggtattgatt gggctgaaca agcctatctg
      181 gatggattgc tgcccgcctg cggcacagat tctgcaacag gcaagccgat gttctgccca
      241 agtcaattgg tggatcgcgg ttggggcgcg tatttgatcg tcaaagccaa gaacctgccg
      301 ttgccgtaag ccatgaggtg acagtcactt tacaagtgac tgtcacctgt ctttgcatat
      361 agggagcgaa gtataatttc cagccatgaa ccaacgtcaa cttaccttgc tgcttgtgga
      421 tgatgatgcc atgatcgttg aaagtttggg gccattgctt gaacgttcgg ggtttcatgt
      481 gataaccgcc tcaacaggtg aagaagcgct taccaaggtg caggctcacc atcccgatgt
      541 gatcgtgatg gatgtgctca tgccgcgcat gaacgggcgc gaagccctac ggcggatgcg
      601 caaatccaat atttggattc cgaccatttt gcttacgcaa gtgggagagg cttctgaacg
      661 cgcggtggct ttggaagaag gcgcagatga ttacctcaac aagccatttc attcgcagga
      721 attgcttgcg cggatccatg ccgtactgcg acgtgcccgc cctgccgggc agtcactttc
      781 ctctgcgcat cgcctcattt cacttgacct gttgctggat cgccaatctc gacgcgcctt
      841 tctcgccgac gaatccctca acctcacacc gaaagcgttt gctgttctcg aatatttgat
      901 gacccacccc gatgaagcgg tcagccgtga gcatttgctg cactcggtct ggggctggga
      961 atacccaacc ggtacacgca ccgtggatac ccgcgtcaac gaattgcgcc gggctttgaa
     1021 agatgaagct ggcgcgcctc gtttcatcca gaccctgacg ggtgaggggt acattttcat
     1081 agcacctgtg aagcctgagt catgagccgt tataaattca tacttccctt tttccctctc
     1141 ctgcttggaa tcctttcctt aatctttttg ccctccctga tcaaacccga ggttgttctc
     1201 tcgtttcggc tggggttggg tgcggttctg tttgggcttg gcctgctcat cacgttgtgg
     1261 atgcagtctt tgggtgtggt cgattcgctg cgtgaaaaga agatgcgcac cgaaatggaa
     1321 gcgctgaccc gcagttatga agacagtcac cgccgcttca ttcgccgcct cgaccacgag
     1381 ttgaaaaatc cgctgatggg tttgcaggct tcgctggaaa atctgcaagc cgtgaccgaa
     1441 ttggaagaac gccgcaaggc ggagggcaat attcaaaaag cccttgagcg gcttgtccgt
     1501 ctgttgcgcg acctgcggaa actctctgac ctggatgaaa gtaaattgga acgcgaatcg
     1561 gtggatatcc ccgaattggt ggaggagatc atcgaggtgt tgcggtccac gccggggcgc
     1621 gaacatcgcc gcattaattt gatggtgact agagtgccca caccgcctcc gccggtgagc
     1681 ggtgaccgcg acctgcttgg catcagcttg tacaatctcc ttgataacgc gcttaagttc
     1741 acggtccaga atgaagcggt tgaagttcgg gtgcgtgatg acgaacgggt ggtctttgtt
     1801 gaagtggcag atggtggttc ggggattcgt ccagatgaac agcataaggt ttttgaggaa
     1861 ctgtatcgtg gcgaaaatgc ccatgccgtg gaagggagcg ggttggggtt gtctttcgtc
     1921 aaacgcatcg ttgcactgca tcacggggag ttgaccttgc gaagccagcg cgatgagcag
     1981 cacggttcca tttttaccat tcgcctccct gttaccaaac tgtgacatgc ctgtgtcatg
     2041 cctgtaacat tcattcgata taactgggtc attcagtaac ttaccgacag tggcctgcgg
     2101 tccactgtca acggtcttta ttgatgcaac tatgaacaac ataacggcaa aacatatcct
     2161 gtattcgctt ctgcttgccg gggtggcatt gctgggcgtt tattttcttc ccactggcaa
     2221 cacatttcaa attaccgtgg aaacccccac gtcgatcgcc tccagcccga tgattaccgg
     2281 agtcagcggc gattttctct gggcaaaacg catgggcgga agcggtaccg gcgatttcgg
     2341 gcaggggtac gatatcgcaa cggatttgga cggaaacctc tatttcaccg gtgggtttac
     2401 cggcgaactg gattttgacc ccggtgaggg cacctttacc ctggccagtg ccagccttga
     2461 ttacagcgat atcttcgccg ccaagttgga ttctgaaggg aattttctct gggcaagaca
     2521 tatcggcggg caggcttggg attatgccta cgatattgcc gtggattcaa acagggatgt
     2581 atatgtgatg ggcagctttg aaggcaccgc cgatttcgac ccgggcccgg atattttgaa
     2641 cttgagcagc gcaggcctgc gtgatatttt cgtggtccgt ctcgatgaaa atggaaattt
     2701 gatctgggcc aaacgcatgg gcggcgcaag tgatgacggc ggttctgctg tggctgttga
     2761 tcacgatggg aatgtatatc taacgggtta ttttcaaggc accgccgatt ttgaccccgg
     2821 ggaaaatacc gtaaacctga aaagcgccgg gaaaaacgat attgttgtta caaaactgga
     2881 tgccgatgga aacttgatct gggcaaaacg catgggtgga attcaaaacg accttggctt
     2941 tggccttgct cttggtgccg attccgtcgt gtacaccact ggccgtttcg aaggcgctgt
     3001 tgattttgac cccggcgctg aaacgactaa ctttgtcagc ataggaggga gcgatgcctt
     3061 cctttcaaag ctggatgccg atggaaattt tgtttgggcg ggtgtggttg gtggaaatgg
     3121 cgaggcgcaa ggctttggcc tggttgtgga ttcaggcggc agcgtgtatt tgaccggcga
     3181 tttttggcgc aaggtcgatt ttgatcccgg cgaaaaatca ttcgacctga ccagtttcgg
     3241 cgattcggat gtctttctgt gtaaattgga tgaaaacgga aatttcgtat gggccaaaca
     3301 aatgggcggc ccgggggctg ggcatggata cgacctcgca cttgacccct cgggcaatat
     3361 ctacaccgtg ggcagtttct cagataccgc cgattttgac cccggcgcgg gtgagtttca
     3421 tcttgcgggc aatggccgct acgaagatat tttcatcgcc caactggatg ccgatggaaa
     3481 ttttatctgg gccaagagcg caggcggcgc gggggatgac cttggtgtgg gcatcgctgt
     3541 agacccgcgc ggcgatgtgg ttgccaccgg taactttgca ggcaaggttg atttcgaccc
     3601 cggcccggag ccgttttacc ttgccagtca gggctactat gatgtttttg tttcaaaact
     3661 taatgggaat ggaataccga tctttgcaga tgtgggtgac gaccatccac agttcaaata
     3721 cattcaagcc ttatacgctt ccggactcac ctcgggttgc gaggtcaacc cattaaagtt
     3781 ctgccccgat gccgtgttaa accgcgccca aacggccgtt ttcatgctgc gtggacagtt
     3841 tggccccgcc tacactccgc ctcctgcccc gtgggatgtc ttttcaacag aagattggag
     3901 cgggttcgaa tgggcacgtc cgtgggccga aggaatgtat gccgccgaat tgaccaaggg
     3961 gtgtcaggct gacccgttga aattttgtcc cacggcacaa ctcacccgca tggaggcttc
     4021 gatatttggg ttgaaaatga aatatgggca gagttacctc cctccgcctg ccactggaga
     4081 tctatttgcc gacctgcctg ccatcggccc gaatgagtgg ggcattgcct gggctgagca
     4141 agcttatcga agcggtctgt tgccttcctg cggaatccaa aatggcaagc ccatgttttg
     4201 cccggatga
//
LOCUS       DAOQLC010000237         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10415_length_4185_cov_24.081598, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000237 DAOQLC010000000
VERSION     DAOQLC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_4185_cov_24.081598"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PL000_20950"
     CDS             <1..411
                     /locus_tag="PL000_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91407.1"
                     /translation="MNGKAWQVMIQGLMENVTVIDAPNDSSPKGQLMEYLERYCTQRA
                     QALSKEEIRLGKPYTEDSRHYFTLSGFMAFLERHKFKLMSMHEITSYLKNDLKAKHHF
                     FNIKGKGTNAWSIAQLGQETKLDVPDTWKNGGPF"
     gene            408..1826
                     /locus_tag="PL000_20955"
     CDS             408..1826
                     /locus_tag="PL000_20955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNC91408.1"
                     /translation="MSNVNIIVGPPGTGKTTTLLNLLDDYLGRGVSPKDVGYISFTVK
                     AADEAKSRATERFGFRKDELPWFRTLHSLAFRWLRLDTKMIMKRQHYSQICEKLGIEY
                     SGFMVLDEGVMLPGGYAGDRMLFHEGLARVRMISLAEQYNRSTEDFSLSELSRLKGAL
                     EAYKDSYSLMDFNDMLTLMLAEKSLPRFKVLFIDEAQDLSRLQWAVVHKLIANSEQVY
                     IAGDDDQAIFAWAGADPDSFINMPGNVTTLAKSYRLPKAVHRLSEAVIRGVSNRRPKE
                     FSSRDEEGLVSFVSDISDIDMSQGDWLVLARNKYLLNPAEQLCEMEGFAYDSKDSPMR
                     TDQAAAIRAYENLRAGKDITDDDRALIKKYGAKVDGTGPIWHEAFTRLGQDVKDYFIA
                     CLRRKESITKTPRIKLSTIHGAKGGEATNVVIYSDMSYKSFEEMTNDPDNELRVFYVA
                     ATRAKQNLFIIEPRTLNSFNWA"
     gene            1914..3362
                     /locus_tag="PL000_20960"
     CDS             1914..3362
                     /locus_tag="PL000_20960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC91409.1"
                     /translation="MPINQDLFKTKPFAHQLDCLEKSWEMKNYALFLEMGCGKSKVFI
                     DTAAQLYHAGKIDAVLLIAPKGVYMNWVDKELPAHWTDNIPIISSYWQSPAKPELQDE
                     WRKFKTFKGLRWMSMNVEAFSYESGLKFASSFINAYGERLLIGIDESTTIKNITAQRT
                     KNIIALGKKAAYRRIMTGDPVPKGPIDMYSQCCFLSPHLLGYTSFYAFRARYCIMVER
                     TGGNRSFKMITGYQRLDELKESIKPFSYRVTKDECLDLPPKVYQTWDVELSKEQLKLY
                     KQMKDEAVALLRGGEVVTAPMVITQLLRLHQITCGFLKTDDGTEIDLPNPRIDALMDV
                     LAGTSGKVIIWATYRNNIKKIFKALEDEYGKESVVSYYGDTDSDERRAAVQGFQNGAV
                     RFFVGNPATGRFGLTLTASASVVYFSNSYDLEHRTQSEDRVHRIGQTASSVNYVDLVC
                     RNTVDERIIKALRSKRKISAIINGDELREWFS"
     gene            complement(3683..3940)
                     /locus_tag="PL000_20965"
     CDS             complement(3683..3940)
                     /locus_tag="PL000_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91410.1"
                     /translation="MEIDLDKLGALIDGGVQAPHDGDDAGHDDWLGGNYADDNPLVQL
                     RLENARLRSALDEANQCIRMVRSILGGVATHTPPHTVHRGV"
     gene            complement(3944..>4185)
                     /locus_tag="PL000_20970"
     CDS             complement(3944..>4185)
                     /locus_tag="PL000_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91411.1"
                     /translation="VDGIAQKIDQLSEAVGALHTELRAHVAEESPLAPAVQELVTVWR
                     GSKIMLPFVAGAITFIISVGGVFIAAATWLKDHVR"
BASE COUNT         1063 a         1109 c         1078 g          935 t
ORIGIN      
        1 atgaatggca aggcctggca ggttatgatt cagggcctca tggagaatgt gacagttatc
       61 gatgcgccta atgacagctc gcctaagggc cagctcatgg aatacctgga gcggtactgc
      121 acgcaacgtg cgcaggctct atctaaggaa gagattcgcc ttggcaagcc gtacacagaa
      181 gacagccggc actacttcac cctgtctggg ttcatggcat ttctggagcg ccacaagttc
      241 aagctcatgt ctatgcatga gattacctca tacctgaaga acgacctgaa ggcgaagcat
      301 cacttcttta acatcaaggg caagggcaca aatgcctggt cgattgccca actgggtcaa
      361 gagacgaaac tcgacgtccc ggacacgtgg aaaaacggag gaccattttg agcaacgtta
      421 acattatcgt cgggccacct ggcaccggca agacaacaac ccttctcaat ctgctcgacg
      481 actatcttgg tcgaggcgtg agtcccaagg acgttggcta tatctcattc acagttaagg
      541 ccgcagatga ggccaagtcg cgagccactg aacgcttcgg ctttcgcaag gatgagttac
      601 cttggttccg aactctccac tccctggcgt tccgctggct gcggcttgat accaagatga
      661 tcatgaagcg ccagcactac tctcaaattt gtgagaaact cggtatcgag tacagcggct
      721 tcatggtctt ggatgagggg gttatgctac ctggtggata cgccggcgac cgcatgctct
      781 tccatgaagg actcgcacgt gtacggatga tctcactcgc tgagcagtac aatcggtcta
      841 ctgaggactt ctctctatcc gagttgtccc gactcaaggg tgcactggag gcttacaagg
      901 attcgtactc tttgatggac ttcaacgata tgcttactct aatgctagcc gagaagagcc
      961 tgccacgctt caaggtgtta ttcattgatg aggcgcaaga cttgtcacga ctccagtggg
     1021 cggtggtcca caaacttatt gccaatagcg agcaggtata catcgccggc gacgatgacc
     1081 aggccatttt tgcgtgggca ggagctgacc cggactcatt catcaacatg cctggcaacg
     1141 taaccacgct ggctaaatcg taccgcttgc caaaagccgt gcatcgtctg tctgaggcgg
     1201 tgattcgcgg tgtatctaac aggcgtccaa aggaatttag ttctcgcgat gaggaaggcc
     1261 tcgtatcctt tgtctccgac atctccgaca tcgatatgtc acagggcgat tggctggtgc
     1321 tcgcacgcaa taaatatctg ctgaaccctg ccgagcagct ttgcgagatg gagggttttg
     1381 cctatgactc gaaagactcc cccatgcgca cagaccaggc ggcagctatt cgtgcatatg
     1441 agaacctgcg cgccggcaaa gatattacag atgatgaccg agcgctgatc aagaagtacg
     1501 gggccaaggt tgacgggact ggaccgatat ggcatgaggc ctttacccgc ctaggccaag
     1561 acgtgaaaga ctacttcata gcgtgcttac gccgtaagga gtccattacg aagaccccac
     1621 gtattaagct gtcaactatt catggagcca agggtggtga ggctaccaat gtggttatct
     1681 actcggacat gagctacaaa tcgtttgagg aaatgaccaa tgacccagac aacgagctac
     1741 gtgtattcta cgtcgcggcc acacgagcca agcaaaacct attcatcatt gagccaagga
     1801 cactaaattc attcaattgg gcataaatcc tgtatattcc ttgactcgga gagtctcgga
     1861 taatatacta gccttattat acccatagac cactttcatc cgaggatatt aaaatgccaa
     1921 tcaatcagga cctgtttaag acaaaacctt ttgcccacca gctagactgc ctcgaaaagt
     1981 cctgggaaat gaagaactac gccttattcc ttgagatggg ctgtggtaag tcgaaagttt
     2041 tcatcgacac ggcagcccaa ttgtaccacg ctggcaagat cgatgcagtt ctactaattg
     2101 cgcctaaggg cgtgtacatg aactgggtgg acaaggagtt gccggctcac tggaccgata
     2161 acatcccaat tatcagcagt tactggcaat cgcctgctaa acctgaacta caggacgagt
     2221 ggcgcaagtt caagacgttc aagggtctac gctggatgag catgaacgtg gaggcgttca
     2281 gctatgaatc agggcttaag ttcgcctcga gcttcatcaa tgcgtacggt gagcgtctgc
     2341 ttatcggtat tgacgaatcc accacgatca agaacatcac agcgcaacgt acgaaaaaca
     2401 ttatagccct tggaaagaag gccgcgtacc gccgcataat gaccggtgat cctgtgccca
     2461 aggggccgat tgatatgtac agccagtgct gcttcttgag tccacacctc ctaggttaca
     2521 cgagcttcta tgccttcaga gcgcggtact gcatcatggt agaacgtacc ggcggcaacc
     2581 gctcgttcaa gatgatcact ggctatcaac gcctagacga gctgaaggag tccatcaagc
     2641 cattctccta ccgcgttaca aaagacgagt gtctcgacct gccgcctaaa gtttaccaga
     2701 cctgggacgt ggagctgtcg aaagagcagc tcaagctgta caagcagatg aaggatgagg
     2761 cggtggcctt gctccgcggc ggagaggttg ttaccgctcc gatggttatc acgcaactat
     2821 tgcggctcca tcaaattacg tgcggcttct tgaaaactga tgatggtaca gagattgacc
     2881 tacccaaccc ccgcattgac gcgctcatgg acgtcctcgc tgggaccagt gggaaggtta
     2941 tcatctgggc gacgtatcgt aacaacatca agaaaatctt caaagccctt gaagacgagt
     3001 acgggaagga gtctgtcgta agctactacg gagataccga tagcgatgaa cggcgggccg
     3061 ctgtacaagg gtttcagaat ggggccgtcc gcttctttgt cggtaatcct gcgaccgggc
     3121 gctttggtct gacgctgaca gctagcgcgt cagtggtcta cttcagcaac tcatatgatc
     3181 tggagcatcg tacacagtct gaggaccgcg ttcaccgcat cgggcaaaca gctagctcag
     3241 taaactacgt cgacctcgta tgcagaaaca ccgttgacga acgcattatc aaggccctga
     3301 gaagcaagcg caagatttca gcgatcatca acggtgatga actgagggag tggtttagct
     3361 gatattgcgt tcgaatagga gacaaagata ggtgtgtgtt tgagattgcg ggccagaccg
     3421 atatcagaac agatagatga caggcggttc agcaacgaag acccgcagca ccgtgtgcgg
     3481 ttaacgctca actcatgtca ttgcccaagg cggcgcgctg gagcgtcgag catagcgcac
     3541 agttcaactc gatacccgtt gatcgcagca catgcaatca tcaaaggcgc ggccaatagc
     3601 cgcaacggac atagcgccag caccctattg actcttactg ttcggacgta ctcatcagtc
     3661 tcagatagca gtgggcgcat aactagactc ctcggtgaac agtgtgcggt ggcgtgtgcg
     3721 ttgctacccc tcccaggatt gaacgcacca tgcgaatgca ctggttcgcc tcatccagcg
     3781 cgctacgcaa gcgcgcgttt tccaaacgca attgtacgag tgggttgtca tccgcgtagt
     3841 tacctccaag ccagtcgtca tgccccgcat cgtcgccgtc atggggcgcc tgtaccccgc
     3901 cgtcaataag cgcgccgagt ttatcaagat caatttccat atcttacctc acatggtcct
     3961 ttagccaagt agcggcggcg ataaatacgc cacctacact gataatgaat gtaatcgctc
     4021 ctgccacaaa tggcagcatg atcttactgc cacgccacac agttaccaat tcctgcaccg
     4081 ccggagctag cggtgattct tctgcaacat gggcacgcaa ttccgtgtgt agtgcaccca
     4141 ctgcttccga caattgatca atcttttgtg caataccatc tacac
//
LOCUS       DAOQLC010000238         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10546_length_4153_cov_22.182528, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000238 DAOQLC010000000
VERSION     DAOQLC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10546_length_4153_cov_22.182528"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PL000_20975"
     CDS             <1..326
                     /locus_tag="PL000_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91412.1"
                     /translation="PSTFNLQPSTFIMTLPTILFALVVALLLGAMYHFFRGGGGWRLM
                     LFFGLSILGFALGQLLAMWQGWRILMFGSLDLGMGGVGSLLMLVTGEWLSRIDVKEIK
                     KESGV"
     gene            401..1036
                     /locus_tag="PL000_20980"
     CDS             401..1036
                     /locus_tag="PL000_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNC91413.1"
                     /translation="MEFLKSAIDLFLHLDEYLAKIISDYGTWTYGILFAVIFVETGLV
                     VMPFLPGDSLLFAAGTFAALGSLNVWFIVGLLSVAAVLGDAVNYSIGHYLGERAYNIK
                     WIKREYLDKTHAFFEKHGGKAIFLARFVPIVRTFAPFVAGIGKMSYGYFATYNIVGGI
                     SWVLIFTLMGFFFGNIPFVKANFELVIIAIILVSVVPMIYEWWKHRQEAKK"
     gene            1090..1452
                     /locus_tag="PL000_20985"
     CDS             1090..1452
                     /locus_tag="PL000_20985"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNC91414.1"
                     /translation="MKYAAVLFTIFIILVIILADRGLLGPLHFIYDFPYGDKAGHFIL
                     YGLLAFFITLASIRSFLRLDPKRVALSIGLILALLIGAEEYSQKFFANRTFDLIDLLA
                     SYIGLIVGGWAALKQARK"
     gene            complement(1522..4086)
                     /locus_tag="PL000_20990"
     CDS             complement(1522..4086)
                     /locus_tag="PL000_20990"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNC91415.1"
                     /translation="MKKLGRFVGRMLIGVVVLALIAGAGGMYYFKSYLPNTVAPKSFP
                     QIDGEISVSGLNGQVDIYRDAMGIPHIYADTTHDLFFAQGYVHAQERFWQMDAWRHIG
                     SGRLSEMFGSGQADTDAFLRTLGWKQTAEEEWKQLDPESKAILEAYVEGVNAYLKDHS
                     DTALSLEYAILGLLSPDYEIEEWTPVNSLTWGKAMAWDLRGNMGEEIERAVLLKTLTP
                     EQVAQLFPAYPEDHPVIVNNIGEGGSAASAGSEATLDYSQVPFENISENVALLDSFLG
                     PWNDGIGSNSWAVSGKLSDTGMPLLANDPHLGIQMPSIWFQVGLHCNEKTAECPFEVA
                     GFSFAGVPGVIIGHNDQIAWGFTNVGPDVMDLYIERVNPENPNQYDVNGTWVDFDTRE
                     EVIKMSDGTTQTITVRSTRHGPVISDQYGPLKDVGDPEDTEFVPFKDRVSTELPEQYV
                     ISLAWTALIPSTPFEAIWGFDKARNWKEFRKAASGFHVPAQNLIYADIEGNIGYQMPG
                     DIPIRAKGDGTLPVPGWTDEYEWQGFLPFEELPYTLNPEEGYIVTANNQVHPRDYPYF
                     ISADWDYGFRANRIVEMITTAPGKIDIAYIQQMHGDSFDANGPVFVPLLLNEEFASDL
                     NPALDTLKNWDYQGRADSTSAAVFNAFWRALLQNTFNDDMPEERYYPDGGSRWNEVMR
                     HMAENPNDPFWDDKTTPDVTETMQDMIQKSFPQGIAELQEIFGKDMSKWKWGDMHAST
                     FRNGTLGESGVSLIEDLFNRGPFPTSGGEAIVNATGWSVKDGYETNWLPSMRMIVDLG
                     DLNNSVTVHTTGESGHAYHPHYADMAPMWAGIQYYPMWWNQDSVIGDAEGHLILKP"
BASE COUNT          883 a         1010 c         1132 g         1128 t
ORIGIN      
        1 aaccttcaac cttcaacctt caaccttcaa ccttcatcat gacccttcca accattctct
       61 ttgcccttgt tgttgccctt cttcttgggg cgatgtatca tttttttcgt ggcgggggcg
      121 gctggcgttt gatgttgttc ttcggtttga gtattctcgg ttttgcgttg gggcagctgc
      181 ttgccatgtg gcagggctgg agaattttga tgtttggatc gctcgatctg ggaatgggcg
      241 gggtgggtag tttgttgatg ctggtgactg gggagtggct gagccgaatt gatgtgaagg
      301 aaatcaaaaa agaaagcggg gtataatccc cgcccgttaa aaacggagcc ctgggttttc
      361 aggcctggcc tccgatatct attgaaagag agaataaaaa atggaatttc tgaaaagcgc
      421 tattgacctc ttcctgcatt tggatgagta ccttgccaag atcatttcgg attacggcac
      481 atggacatac ggcattttgt tcgctgtgat ctttgtggaa acagggttgg tggtcatgcc
      541 ctttctgcct ggcgattcac tgctctttgc agcaggaacg ttcgctgcac tgggttcatt
      601 aaatgtgtgg ttcattgtgg gtttgctttc ggtggcggct gtgctgggtg atgcggtgaa
      661 ttactccatc ggtcattatc tgggcgagcg cgcctacaac atcaagtgga tcaagcgcga
      721 atatctcgat aagacccacg cctttttcga aaaacatggt ggcaaggcca tctttcttgc
      781 gcgcttcgtt cccattgtgc gcacctttgc gccgtttgtg gcagggatcg gtaaaatgtc
      841 ctatggctat ttcgccacct acaacattgt gggcggaatt tcctgggttt tgatctttac
      901 cctgatgggt ttcttcttcg gaaacattcc ctttgtgaag gcgaatttcg aattggtcat
      961 cattgccatt atccttgtct cggtggtgcc gatgatctac gagtggtgga agcatcgtca
     1021 ggaagcgaag aagtaatttc gaaatcgcgc gccgtgccat ttgtgtggcg cggcgcgttt
     1081 tttatctcca tgaaatacgc tgctgttctt tttacgattt ttattatttt ggtcatcatt
     1141 cttgcagacc gtggattgct ggggccgctc cactttattt acgattttcc ctacggtgat
     1201 aaggcgggac acttcatcct gtacgggctt ctcgcctttt tcatcacact tgcctccatc
     1261 cgctcgtttc ttcgcctgga cccgaaacgg gtggcgcttt caattggctt gattctcgcc
     1321 ctgctcatcg gcgcagagga atattcacaa aagttcttcg ccaatcgcac ctttgacctg
     1381 attgacctgt tggcgagtta tattggtttg atcgttgggg gatgggcagc cttgaaacag
     1441 gcacgcaaat aaaaaaatct ttcctctttc ttcgtttatt gaagaaagag gaaagatata
     1501 agaaattcag agtgtttttt actatggttt caagatcaag tgtccttcgg catcgccgat
     1561 gacagagtct tgattccacc acatggggta atactgaatg cccgcccaca tgggagccat
     1621 atcggcatag tgcggatgat aggcatggcc tgattcgcct gtggtgtgga cggtgacgga
     1681 attattcaaa tcacccagat ccacgatcat gcgcatggag ggcagccagt tggtttcgta
     1741 gccatccttc accgaccagc ccgtggcgtt gacgatggct tcgccgccgc tcgtggggaa
     1801 gggtccacgg ttgaagaggt cttcgatcaa cgagacgccc gattcgccga gggtgccatt
     1861 gcggaaggtg gaggcgtgca tgtcacccca tttccatttg gacatgtcct tgccgaagat
     1921 ttcctgcaat tcggcaatgc cctgcgggaa ggatttttgg atcatatcct gcatggtttc
     1981 agtgacatca ggggtggtct tatcatccca gaacgggtcg ttggggtttt cggccatgtg
     2041 acgcatgacc tcgttccagc gtgagccgcc atcgggataa tagcgttctt caggcatgtc
     2101 gtcgttgaag gtgttttgca gtaaagcgcg ccagaatgca ttgaacacgg ccgcggatgt
     2161 ggaatctgct cggccttggt aatcccaatt ctttagcgta tccagcgcgg ggttaaggtc
     2221 agatgcgaat tcttcattca gaagaagcgg tacaaagaca ggtccgttcg catcgaagga
     2281 gtcgccgtgc atttgttgaa tgtaggcgat atcgatcttg cccggggcgg tggtgatcat
     2341 ctcgacaatg cggttggcac ggaagccgta gtcccaatcg gcagagatga aatagggata
     2401 atcacgcgga tgcacctgat tgttggcggt gacgatgtag ccttcttcgg gattgagggt
     2461 gtaaggcagt tcttcgaaag gaaggaaacc ctgccattcg tattcatccg tccaaccggg
     2521 gacgggcagt gtgccatcgc ctttggcgcg aatgggaata tcgcctggca tttgatagcc
     2581 gatattgcct tcaatgtcgg catagatcag gttttgcgca ggtacatgga atccactggc
     2641 ggctttgcgg aattccttcc agttgcgcgc cttatcgaat ccccatatgg cttcgaatgg
     2701 tgtggagggg atcagcgccg tccatgccag ggagatgaca tattgctctg gcagttcagt
     2761 gctcacacgg tctttgaatg ggacgaactc agtgtcttcg gggtcgccca catctttgag
     2821 cggaccatat tggtcggaga tgacgggccc atggcgggtg ctgcgcacag tgatggtctg
     2881 cgttgtgcca tcagacattt tgatgacttc ttcgcgggta tcaaaatcca cccaggtgcc
     2941 gttcacatcg tattgattcg ggttttcagg gttgactctt tcgatgtaca agtccatcac
     3001 atcaggcccg acattggtga agccccaggc gatctggtca ttgtggccga tgatcacacc
     3061 gggtacccca gcaaatgaaa agcccgccac ttcgaaggga cattcagcgg ttttctcatt
     3121 gcagtgcaag ccgacctgaa accagatcga aggcatttga atgccaaggt gtggatcgtt
     3181 tgccagcagc ggcattcctg tatcagagag tttgcctgaa accgcccacg aatttgaacc
     3241 gatgccatca ttccacgggc caaggaaaga atcgagcaaa gccacatttt cagaaatgtt
     3301 ttcaaacggc acttgtgaat aatcaagggt cgcttcgctg cctgcacttg cagccgaacc
     3361 gccttcgcca atgttgttga caatgacagg atggtcttca ggataggcag ggaaaagctg
     3421 tgccacctgc tcaggggtga gcgttttgag cagaacggca cgttcgatct cttcgcccat
     3481 gttgccgcgc aggtcccaag ccatggcctt gccccatgtc aggctgttga cgggagtcca
     3541 ttcttcgatt tcgtaatccg ggctgagcag ccccaggatg gcatattcaa ggctgagggc
     3601 tgtatcggag tgatccttca aataagcgtt cacaccttct acgtaggcct ccaaaatggc
     3661 ctttgattca gggtcgagtt gcttccactc ttcttcggcg gtttgcttcc agcccagtgt
     3721 gcgcagaaat gcatctgtgt cggcttgtcc gctgccgaac atttccgaaa ggcggcccga
     3781 gccaatgtgc cgccatgcat ccatctgcca gaagcgttcc tgcgcgtgga cgtatccctg
     3841 cgcgaaaaat agatcgtggg ttgtatcagc atagatgtgc gggatcccca ttgcgtcgcg
     3901 gtagatatcc acctgaccgt tgagtccgct tacgctgatc tcaccgtcaa tttgcggaaa
     3961 ggattttggc gcgacggtat tcggtaaata agacttgaaa taatacattc ccccggcgcc
     4021 tgcgatcaac gcgagaacga caacgccgat caacattcgc ccaacgaacc tgcctaactt
     4081 tttcatgctt cctccagttg gaaaattttg tggattatag tcacctgtgg gtggattgca
     4141 tagcacataa aat
//
LOCUS       DAOQLC010000239         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10703_length_4121_cov_29.603542, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000239 DAOQLC010000000
VERSION     DAOQLC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10703_length_4121_cov_29.603542"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1291
                     /locus_tag="PL000_20995"
     CDS             2..1291
                     /locus_tag="PL000_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91416.1"
                     /translation="MTTAISVKNIGKQYKIGAAETKFRYNMLRDVIVDTVSAPIRLAK
                     AMIGRSDRRLNQNFVWALKDVSFDLEEGKVLGIVGRNGAGKSTLLKILSRVTEPTTGT
                     VSVRGRVGSLLEVGTGFHPELTGRENIYMNGAILGMRRTEIDSKFDEIVDFSEVSQFI
                     DTPVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDAEFQKKCLGKMGDVAQQGRT
                     VLFVSHNMSAILRLTQEAIVLNKGQLIMRGPTQEAVDYYLSSGQSQAGERTWGAEDVP
                     AAAMPFTPISLKVKERSGKVVDTIRSTEPVTVEFEYKLGAPVTGLRVGLYVSTMRGEY
                     VFTSFDTDSPELYENFDSRNAGHYISRAELPADIFNEGRYTVGVNASSFGVRRYFMDE
                     NALAFNVDISGAPGTQWAEPRVGPVRPRLNWKIEKIS"
     gene            1354..2988
                     /locus_tag="PL000_21000"
     CDS             1354..2988
                     /locus_tag="PL000_21000"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91417.1"
                     /translation="MTNTLRNLLGDLPFTAEIDWMLRSKNRPRKDHYNLGRLEKSLPA
                     AVEVVKPFAEKAPRGKKVLFFATLHYWIEQSAYLGLVLAGLGHDVTLLTLPYSEWHKE
                     KDKFTQRQRVLHTHDSLAVLSPLVKHVSMLDMQSSARVSHGTLRAVKHDDLPEKILAD
                     IKEVSLWDVQYTLMREEVDMNDAGDRALYDLRIERNTFAAKAALQFMQTEKPDVVLIP
                     NGLILEMGIVFRVARHLGIDAVTYEFNDQREQIWLAQNSSIMQQETDYLVDARCSLPM
                     TDAMFERVADLENARRGARVWGKSKRLWQYVSSQGAAETRKMLNLDDRPVVMLAANVL
                     GDSLTLGRDIFASSMTEWITKTVQYFAKRTDVQLVIRVHPGEKLVPQAKSMATVVKDA
                     LPELPSHIHVIGALDKVNTYDLIEIADVGLAYTTTVGLETTMNGRPVISCGRTHYRGR
                     GITIDPNTWEEYYATLEKVLSDIPAHQMTEKQIEFAWNYAYRFFFEYPRPFPWRLMNF
                     WDDLAEHPLESVLSEEGKAQFKDTFDFLVGEPFSWK"
     gene            3058..3879
                     /locus_tag="PL000_21005"
     CDS             3058..3879
                     /locus_tag="PL000_21005"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC91418.1"
                     /translation="MTNETKQKVREFYDEIGWMQEDDGNYQNARYEDLRPVSREYIHK
                     TRLRVMNGLIPTGRFMLDAGSGPVQYEEYKTYSAGYEKRVCLDISIQALREARTRIGD
                     HGLFVVGDLANLPFKKDAFDGAVSMHAIHHLALPEHPRAYAEIHRVLAPGRTAAIVNG
                     WSDPLISRMAEPFIGLLRKVTGRDAKKKKEWLSEDAPAGTFVEKMSPAWLKREIGSKM
                     TIELKPWRGLSTRILRNFIRPFGGRAFLKFVFWLEGIFPKFFAENGQYPLIVVKK"
     gene            3881..>4121
                     /locus_tag="PL000_21010"
     CDS             3881..>4121
                     /locus_tag="PL000_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91419.1"
                     /translation="MQRFTDQQYLTTDQYKDSANLDARIAIHQKFSTNPQGWFNWVFD
                     TLVTLPADAHVLELGCGAGTMWNQCADRIPAGWTIT"
BASE COUNT          953 a         1117 c         1153 g          898 t
ORIGIN      
        1 tatgacaaca gcaatcagcg taaagaacat tggcaagcaa tacaagatcg gagcagcgga
       61 gacgaaattc cgctataaca tgctccgtga tgtgatcgtg gatacggtct ctgcgcccat
      121 tcgtctggcg aaggcgatga tcggcaggtc agaccgccgc ttgaaccaga attttgtctg
      181 ggcgttgaag gatgtttcct ttgacctcga agaaggcaag gtactcggca ttgtagggcg
      241 caacggcgcg ggcaagtcca cgctgttgaa gatcctctcc cgagtgacag aaccgaccac
      301 gggcaccgta tctgtgcgcg gacgggttgg ctcgctgctc gaagtgggca caggtttcca
      361 ccccgagctg acgggacgcg agaacatcta catgaacggc gccatccttg gcatgaggcg
      421 cacggagatc gactcgaagt tcgatgagat cgtggatttt tccgaggtca gccaattcat
      481 tgacacccct gtcaagcgtt attcgtcggg catgtatttg cgccttgcgt tcgcggtggc
      541 tgcacacctt gagcctgaga tcctcgtcgt ggatgaagtg ctcgcggtgg gtgacgccga
      601 attccagaag aagtgtctcg gcaagatggg tgacgttgcc cagcagggac gcacggtctt
      661 gttcgtcagc cacaacatgt ccgcgatcct tcgtctgacg caggaagcga tcgtgctgaa
      721 caaaggtcag ctcatcatgc gcggaccgac ccaggaagcc gtggactatt atttgtcatc
      781 ggggcaatcg caggcgggcg agcgcacctg gggcgcggaa gatgtcccag cggcggcaat
      841 gccttttacg ccgatcagcc tcaaagttaa agaaagaagc gggaaggtcg tcgatacgat
      901 ccgctccacg gaaccagtca cggtcgagtt cgagtacaag ctcggcgcgc ccgtcacagg
      961 tctgcgggtg ggactttacg tcagcacgat gcgcggtgaa tatgttttca cctccttcga
     1021 taccgacagc cccgagcttt atgaaaattt cgactcgcgc aatgcgggtc attacatcag
     1081 ccgcgcggaa ttgcccgccg atattttcaa tgaaggtcgc tacactgtgg gcgtgaacgc
     1141 gagttcgttc ggtgtgcgcc gctacttcat ggatgaaaat gcgctggcgt tcaacgtgga
     1201 tatttctggc gcgcctggca cgcaatgggc ggagccgcgc gtgggacctg tgcgtccgcg
     1261 attgaattgg aagatcgaaa aaattagtta gaaagtttca ggttgaaggt tgcaagttta
     1321 accttcaacc tgaaaccttc cacctgaaac cctatgacaa acaccctccg caaccttctg
     1381 ggcgacctgc ccttcactgc cgaaattgac tggatgcttc ggtcgaagaa tcgtccgcgc
     1441 aaagatcact acaacctcgg gcgtttggaa aagtcgctgc ccgcggcggt ggaagtggtc
     1501 aaaccgttcg ctgagaaggc gcctcgcggt aaaaaagttt tgttctttgc caccctgcat
     1561 tattggattg agcaatctgc ttatcttggg ctggtgctgg cgggcttggg gcacgatgtc
     1621 actttgttga ccctgcctta ttccgaatgg cacaaggaaa aggataagtt cacccaacgt
     1681 cagcgtgtgc tgcacacgca tgattctctt gcggtgttgt cgccgttggt gaagcacgtt
     1741 tcgatgttgg atatgcaatc ttctgcgcgg gtttcgcatg gcacgctgcg cgcagtgaag
     1801 catgacgacc tgccagagaa gatcctggcg gatatcaaag aggtttctct ttgggatgtg
     1861 caatacaccc tgatgcgcga agaagtggac atgaacgatg caggcgaccg cgcgctctat
     1921 gacctgcgta tcgaacgcaa cacctttgcc gccaaagccg cgcttcaatt catgcagacg
     1981 gaaaagcccg atgtggtttt gattcccaac ggcttgatcc tcgaaatggg aattgtcttc
     2041 cgtgtggcgc gccacctcgg cattgatgcg gtcacctacg aattcaacga tcagcgcgaa
     2101 cagatctggc tggcgcaaaa ttcctccatc atgcagcagg agacggatta tctcgtggat
     2161 gcgcgttgct ccctgcccat gaccgatgcc atgttcgagc gcgtagcaga ccttgaaaat
     2221 gcccgccgcg gcgctcgtgt gtggggcaaa tcaaaacgcc tctggcagta tgtctcttcg
     2281 caaggcgcgg cagagacccg caaaatgctc aacctcgatg accgccctgt ggtgatgctc
     2341 gccgcgaatg tgctcggtga cagcctcact ctcgggcggg acatctttgc ttcatccatg
     2401 accgagtgga tcacgaagac cgttcaatat tttgccaagc ggacggatgt gcagttggtc
     2461 atccgtgtgc atcctggcga gaagttggtg ccgcaagcca aatcaatggc aacggtcgtg
     2521 aaggatgctt tgcccgaact ccccagccac atccatgtga tcggcgcgtt ggacaaagtc
     2581 aacacgtatg acctgatcga gatcgcggat gtcgggctgg cgtacaccac cacggtcggg
     2641 cttgaaacca caatgaacgg acgccccgtc atctcttgtg gtcggaccca ctaccgcgga
     2701 cgcggcatca ccattgaccc caatacatgg gaagaatact acgccaccct tgaaaaagtt
     2761 ttgagcgaca tccccgcgca tcaaatgacc gagaagcaga tcgagttcgc gtggaattat
     2821 gcctaccgat ttttctttga gtacccgcgt cccttcccct ggcgtttgat gaatttctgg
     2881 gacgacctcg ccgagcatcc actcgaaagt gtgttgagcg aagaaggcaa ggcgcagttc
     2941 aaggacacct ttgactttct ggtgggtgag ccgtttagct ggaaataaat cggagtgcgg
     3001 actttagtcc gcaaaaatac aaaacgcgga ctgaagtccg cgatccgtta ggaagatatg
     3061 accaacgaaa ccaaacaaaa agtacgcgag ttctacgacg agatcggatg gatgcaggaa
     3121 gatgacggca attatcagaa tgcccgttat gaagacctgc gccccgtctc gcgcgaatac
     3181 atccacaaga cgcgcctgcg cgtgatgaat ggattgatcc ccacggggcg gttcatgctg
     3241 gacgcgggct ctggtcctgt ccaatatgaa gagtacaaga cctattcagc gggctacgaa
     3301 aaacgcgtct gccttgatat ttccattcaa gccctgcgtg aagcgcggac tcgaatcggt
     3361 gatcacggct tgttcgtggt tggtgacctg gcgaatctgc ccttcaaaaa agatgctttc
     3421 gacggcgcag tttccatgca tgccatccat caccttgctt tacctgaaca tccgcgcgcg
     3481 tatgctgaaa ttcatcgcgt acttgcgcct ggtcgcactg ccgccatcgt caatgggtgg
     3541 agtgacccgc tcatcagccg catggcagaa ccgttcatcg gtctgctgcg caaagtcact
     3601 gggcgcgatg cgaagaagaa aaaggaatgg ctgagcgaag atgcccccgc gggaaccttc
     3661 gttgaaaaaa tgtctcccgc atggctcaag cgtgagatcg gctcgaagat gaccatcgaa
     3721 ctcaaaccct ggcgcggact cagcacccgc atcctgcgca acttcatccg tccctttggc
     3781 gggcgcgcgt tcttgaaatt tgtcttctgg ctcgaaggta tcttcccgaa attcttcgca
     3841 gaaaacgggc agtacccttt gatcgtggta aagaaataaa atgcaaagat ttaccgacca
     3901 gcaatatctc accacagacc agtacaagga ttctgcgaac ctcgatgcac gcatcgcgat
     3961 ccatcaaaag ttcagcacga atccgcaggg ctggttcaac tgggtcttcg atacactggt
     4021 cacattgcct gctgatgctc atgtgttgga attgggctgc ggcgcaggca cgatgtggaa
     4081 tcaatgcgcg gatcgaatcc ccgcaggctg gaccatcacc c
//
LOCUS       DAOQLC010000240         4099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10821_length_4099_cov_13.030168, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000240 DAOQLC010000000
VERSION     DAOQLC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10821_length_4099_cov_13.030168"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="PL000_21015"
     CDS             <1..1123
                     /locus_tag="PL000_21015"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC91420.1"
                     /translation="TEAPAATEAPAATEAPAAGAENEGEVSIIAWPGYLERGATDPAY
                     DWVTEFEKETGCIVKVQDAATSDEMVRLMSDGGYDLVTASGDASLRLIYGGVVQEIDI
                     TKVPSYSTVDERLQEAPWHFVDGKHYGVPYQWGPNVLMYNTEVFPEAPTSWSVVFEEQ
                     TLPDGQSNKDRVQAYDGPIYIADAALYLKATRPELGIDDPYFLTQEQYNAVIELLTKQ
                     GEIVPKYWHDYLVHMEDFTNEGFVASSSWPIQVNLLQFDGQPIASTIPVEGATGWADT
                     TMMHKDAPHPVCAYLWLEHSLDVKLQGDLAAWFGSVPAVPAACTGNELLTDEGCAVNG
                     FDDFEKIYFWKTPTAECYDGQTCVTYDKWTEDYIAIQGQ"
     gene            1267..2295
                     /locus_tag="PL000_21020"
     CDS             1267..2295
                     /locus_tag="PL000_21020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC91421.1"
                     /translation="MGESAILFKNVSRHFGEVKAVDDVTLEIRDGEFFSMLGPSGSGK
                     TTCLRMIAGFDRPTLGQIFLYGQDVSNLPPYDRSVNTVFQDYALFPHMTVGENIAYGL
                     MIKGIAKDQRKKQVDEMLDLVRLTGFADRKPSQLSGGQRQRVALARALVNHPKVLLLD
                     EPLGALDLKLRQQMQVELKTIQQRVGITFIFVTHDQEEALTMSDRIAVFNLGKIEQVG
                     SPADIYEHPQTSFVAGFVGVSNLIGGEVAKHITGAEDKFSIRPEKIHLSSMDARADAD
                     MYSTTGKVRDVVYLGLYTRYLVELDGGGDLVVVEQNLKSTSMDVMKSKGQQTRLMWKK
                     EHISKLGG"
     gene            2297..3202
                     /locus_tag="PL000_21025"
     CDS             2297..3202
                     /locus_tag="PL000_21025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC91422.1"
                     /translation="MLSRVSTYLYLRPKLVLALLLVPPLLWFLVIYIGSLISLLVNSF
                     FSLDSFTGQIVREFTLKTYAKLFETSNLQIFARTTVMAALVTVACAALAFPLSYYMAR
                     FASPRMKTFLYIGVTLPLWSSYVVRVYSWKLILAQEGIVSWFAHIVRLDGLLDWYLSL
                     PLVGGPSLAISSTGVFLVFVYIWLPYMVIPIQTALERVPKSLIEASSDLGAKPAQTFR
                     NVILPLAFPGVIAGSIFTFSLTLGDFIVPQLFGSSAYTIGRAVLTFQGTSNNIPLAAA
                     FTMGPILIMIVYLLIARKLGAFDAL"
     gene            3192..4028
                     /locus_tag="PL000_21030"
     CDS             3192..4028
                     /locus_tag="PL000_21030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC91423.1"
                     /translation="MLSKNPSKPSTSTERGSWWLQISAIAVLLFLHIPMWLIFLYSFT
                     TDTAAYTFPPPGLTLKWIPQALSNPEMQQALSLSIRVASLATVAALILGTLAAAAVYR
                     SDFFGKEAISFMLLLPLALPGIVTGIAMRSAMSLLSIPFSYWTIVIGHATFCVVVVYN
                     NVLARFRRSGGSMIEASMDLGANSFQTFWHIVLPNIGTALLAGAILAFSLSFDEVIVT
                     TFTAGQQETLPIWILGQMFKPRSRPITNVTAMLVVIITALPILLAQRITAGSSDTEGG
                     NK"
BASE COUNT          893 a         1054 c         1002 g         1150 t
ORIGIN      
        1 cactgaagca cctgctgcaa ccgaagcccc tgccgccacc gaggctcctg ctgccggagc
       61 ggaaaatgaa ggcgaagttt ccatcatcgc ctggcctggt tacctcgaac gcggcgcaac
      121 cgaccctgcc tatgactggg taactgaatt cgaaaaggaa actggttgta tcgtcaaagt
      181 gcaggatgct gccacttccg atgaaatggt ccgtttaatg tctgatggtg gttacgatct
      241 cgtgaccgct tccggtgatg ccagcttgcg cttgatctat ggtggtgtcg ttcaggaaat
      301 tgatattacc aaggttcctt cctacagcac tgttgacgaa cgcctgcagg aagctccctg
      361 gcactttgta gatggcaagc attatggtgt tccttatcag tggggcccga atgtgttgat
      421 gtacaacact gaagttttcc ccgaagcccc cacttcatgg agcgtggttt tcgaagagca
      481 gacccttcct gacggtcagt ccaacaaaga tcgcgttcag gcatacgatg gcccgatcta
      541 cattgccgat gcggctttgt atctcaaggc tacccgcccc gaactcggca ttgacgatcc
      601 ctacttcctc acacaggagc agtacaatgc tgtgatcgaa ttgctcacca aacagggcga
      661 gatcgttccc aagtactggc acgattatct ggttcacatg gaagacttta ccaatgaagg
      721 tttcgtggct tcctccagct ggccgatcca ggtcaacttg ctgcagttcg acggacagcc
      781 gatcgcatcc acgatccctg tggaaggcgc aacgggctgg gccgatacaa ccatgatgca
      841 caaggatgct ccgcaccctg tctgtgctta tctgtggctt gagcactccc tcgatgtcaa
      901 acttcaaggc gatcttgctg cctggttcgg ctctgtaccc gccgtgcctg ctgcctgcac
      961 cggcaatgaa ttgctgacgg atgaaggttg tgcagtgaac ggcttcgatg atttcgagaa
     1021 gatctatttc tggaagacgc cgaccgccga atgctatgat ggtcagacct gcgtcaccta
     1081 cgataaatgg actgaagatt acatcgccat tcaaggtcag taggttttta gaataacaaa
     1141 ttcaagccgc gtggagtttc tccacgcggc ttgaaccttg tttttgggat atgaattatt
     1201 cggaattgtc cgtataattc gtatcccaat cacagtaata aacaaaatag atgagagaga
     1261 atagccatgg gtgaatcagc gattttgttc aagaatgtaa gccgccactt tggcgaagta
     1321 aaggccgtgg atgatgtaac actggaaatc cgtgatgggg aattcttttc catgcttggc
     1381 ccctcaggtt caggcaaaac cacctgcctg agaatgatcg ccggtttcga tcgcccgacg
     1441 ttggggcaga tctttctgta tggtcaagac gtttccaacc tgcctcccta tgatcgttcg
     1501 gtcaataccg tatttcagga ctatgctctt ttccctcaca tgaccgtggg tgaaaacatt
     1561 gcctatggtt tgatgattaa agggattgcc aaagaccagc gtaaaaaaca ggttgatgaa
     1621 atgctggatc ttgtgcgtct tacaggattt gcagatcgga aacccagcca gctttcaggc
     1681 ggacagcgac aacgtgtcgc tcttgcccgg gctctggtca atcatcccaa ggttcttctg
     1741 ctcgacgaac ctctcggtgc attggatttg aaactgcgcc agcaaatgca agtggaattg
     1801 aaaaccattc aacagcgcgt tgggattaca ttcattttcg taacccacga tcaggaagaa
     1861 gcgctcacca tgagcgaccg tattgcagtt ttcaatctgg gcaagattga gcaggtaggt
     1921 tcccctgccg atatctacga acatccgcag acatcatttg tggctggctt tgtcggtgta
     1981 tccaacctta tcggtgggga ggttgcgaaa cacattactg gcgcagaaga taagttctcc
     2041 attcgtcccg aaaaaattca tctttccagc atggatgcca gggcagatgc tgatatgtat
     2101 tccacgacag gcaaggtgcg tgatgttgtc tatctgggtc tttacactcg ttaccttgtt
     2161 gagttggatg gcggtggtga cctggttgtt gtagagcaaa atctcaaatc cacctcaatg
     2221 gatgtgatga aatctaaagg tcagcaaacc cgcctgatgt ggaagaaaga gcacatcagt
     2281 aagttgggag gctaatatgc tcagccgtgt ctcaacctat ttatatttgc ggcccaagct
     2341 ggttttggcc ttattgttag tgccgcccct gctttggttt ttggtgatct acatcggttc
     2401 acttatttcc ctgctggtca acagtttttt ctcgctcgat agcttcactg ggcagatcgt
     2461 tcgtgagttt acgctcaaga cgtatgccaa gttatttgaa acttccaatc tgcagatctt
     2521 tgcccgcacg acggtcatgg ctgcgttggt gactgttgcc tgtgctgctc tcgcttttcc
     2581 gctttcttat tacatggctc gttttgcttc cccgcgcatg aagacatttc tatatattgg
     2641 tgtaacgctc ccactttggt caagctatgt ggtacgtgtg tactcatgga agttgatcct
     2701 cgcgcaggaa ggcatcgttt catggtttgc tcacattgtg cgtctggatg gtttgttgga
     2761 ttggtatctc tccctgcctt tggtcggtgg accgtcactt gccatttcgt ctacaggagt
     2821 attcctcgta tttgtttata tctggctgcc gtacatggtc attccgatac aaacagcctt
     2881 ggaacgtgtg cccaaatctc tgatcgaagc atccagtgat ctgggcgcaa aacccgcaca
     2941 gacctttcga aatgtgatcc tgccgttggc ttttcccgga gttattgcag gctccatttt
     3001 tacgttttcg ctgacacttg gcgacttcat cgttccgcag ttgttcggta gttccgccta
     3061 caccatagga cgggctgtac ttacgtttca gggtacatcc aataacattc cacttgccgc
     3121 tgcgtttacg atggggccca tcttgatcat gattgtgtat cttttgatcg cccgaaaact
     3181 aggagctttc gatgctctct aaaaaccctt ccaaaccctc cacttccact gagcgtggtt
     3241 cctggtggct tcaaataagc gccattgcag tgctgctctt tttgcacatc cccatgtggt
     3301 tgatcttctt atactcattt accacagata ccgctgctta caccttcccg ccgcccggcc
     3361 tgaccctgaa atggattcca caggcattat ccaaccctga aatgcagcag gcgttgagtt
     3421 tgtccattcg tgtggcttcg ctggcaactg tggctgcgtt aattttggga acactcgccg
     3481 ccgcggctgt gtatcgtagt gattttttcg gcaaggaagc catctcgttc atgttgcttc
     3541 tgccgcttgc cttgcctggc attgtcaccg gtattgccat gcgttcggcg atgagtttgc
     3601 tcagcatccc attttcctat tggacgattg tgatcggcca cgccaccttt tgcgtcgttg
     3661 ttgtttataa caatgtactc gcacggttcc gtcgttcagg cggttcaatg attgaggctt
     3721 cgatggattt gggtgcaaat tccttccaga ccttctggca tattgttctt ccaaatatcg
     3781 gcacggcttt actggctgga gccattctcg ccttctcact gtcattcgat gaagtcattg
     3841 tgaccacatt cactgcaggt cagcaggaaa ccctgccgat ctggatcctt ggccaaatgt
     3901 tcaagccgcg tagccgcccc atcaccaatg tcactgctat gctggtagtc atcatcactg
     3961 cacttccgat tttgctggca caaaggatca cggctggctc ttctgacacc gaaggcggga
     4021 acaaatagcc tgcaagtaaa acctggcgag cggcgaggat tctccttgtc gctcgttttc
     4081 tttgtataat ccctcatat
//
LOCUS       DAOQLC010000241         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_10827_length_4098_cov_14.189463, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000241 DAOQLC010000000
VERSION     DAOQLC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10827_length_4098_cov_14.189463"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /locus_tag="PL000_21035"
     CDS             complement(<1..1700)
                     /locus_tag="PL000_21035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC91424.1"
                     /translation="MLKFSVVINTYNRAAYINDAILGLAELNYPDYEVIVINGPSTDR
                     TGEILEGWKERIKIGECAEANLSMSRNIGIQMASGDVVAFMDDDAVPHPEWLKRLAVH
                     YADPSVGGVGGFTIDNTGVRYQVRKTVCDRFGNAFFPHDYFDERPLSFPGTPIYPSLL
                     GTNSSFRLSALREIGGFDHVFAYLLDETDVCLRMIDRGYQIVYEPNALVYHQFAASHV
                     RSKNRIPKTLYPSAISKSYFIHRHGAAFSEVRASEEIGKYENQILNDNKWLSEHGEIT
                     EAHRVSLDDDLLFGIREGGRRALERRMADQGGMGGMGGLDFSVPELPFVQMKEKNGLR
                     IVFVSRSFPPAVEAGIARWTSMMARGFADRGHKVHVVTHAEKEPYTRYQEGYWIHAVT
                     EDHAEEGRLMAARRAIPSGLAGWCYAVYKAVKSLKTFGVDVVSFPIWDVEGILLADDS
                     SLNLVMSLHTSYAMALPFKREWTERPLFSHFHVGRIIAAETRLLSQVRHVLGNSRAIV
                     NDIEKTYGVSIAEKTLIAQHGTFEPIGYSALDIEKKIEDGRGDRPMRIAYVGRFESRK
                     G"
     gene            complement(1704..2504)
                     /locus_tag="PL000_21040"
     CDS             complement(1704..2504)
                     /locus_tag="PL000_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91425.1"
                     /translation="MKFLSRRARPVKQSNGGKEDKSVYLLLEKVRSVGDCLRVFEESG
                     MLDQGFLLEDTVANLHSMCVAMRKKGMAMEPNLIGRAVIQFIVDARQAGAAALRFAGQ
                     DTDFQGLVAEADMMRDSNDFAKAEYFCWRALTLFPKHPLIYVQYAHALKEQGKAADAL
                     VNYLNALIFGAPMLDVEEHALFVAEKLGIKSQLEMRLRSSGSLLPSADVIMLHELILG
                     WSPQVGVVLELMMQHEHAHSIFVELIHEDDFWRANRDLLRFVAETRGV"
     gene            complement(2520..3836)
                     /locus_tag="PL000_21045"
     CDS             complement(2520..3836)
                     /locus_tag="PL000_21045"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNC91426.1"
                     /translation="MSSKKTIMVDGRNLGLEKGTGVSTYARNLSHCLHEMGYGVDVLY
                     GNRAAPGMSDLMKEITFFDDNLRKTPAVLRVLNTAKDLFRTPFGFNATRVPITGKVIS
                     DTFKSRLPYFDHIENSPNIFTKAHNVFGVFKQLNKIYPEQKPDLMHWTYPLALRVPGV
                     PNIYTLHDLVPLRLPYTTLDNKRRYFRLVSAIARRADHIVTVSETSKRDIMELLGVPE
                     ERITNTYQSVHIPEKYVDKPEEVVQREIEGTFGLGYKNYYLFWGSIEPKKNIGRMIEG
                     YLASGVEAPLVVVGAQAWKSEEELKLLNEGAMRDRGGSETSIKRKVIRLSYVPFPLLV
                     SLIRGAKATVFPSLYEGFGLPVLESMLLGTPVIAANTASLPEIAGESAILINPYDSQE
                     IARAIRVLDVDDTIREGYASAGRIQGLKFSKEAYCERLSSIYKRFI"
     gene            complement(3842..>4098)
                     /locus_tag="PL000_21050"
     CDS             complement(3842..>4098)
                     /locus_tag="PL000_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91427.1"
                     /translation="FAISMAVEFDCFLIDEVITVGDARFHEKCQHELFEKRKSRAFVL
                     VSHEAHNIREHCGKALVLHRGKLDQFSNIEEAYSFYQERC"
BASE COUNT         1093 a         1115 c          902 g          988 t
ORIGIN      
        1 ccttttctcg attcgaatcg accaacatat gcaattcgca tcggcctatc gccacggccg
       61 tcctcgatct ttttctcgat atccagagca gagtatccaa taggctcgaa agtcccatgc
      121 tgtgcaatca gcgtcttttc agcaattgaa accccatagg ttttttcaat atcattcaca
      181 attgccctgg agttcccaag cacatgcctg acctggctca gcagacgcgt ctccgcagca
      241 atgattcgcc ctacatgaaa atgagaaaaa agagggcgct ctgtccattc ccgtttaaat
      301 ggaagcgcca tggcataact ggtgtgcaaa ctcatgacca aattcaaaga gctgtcatcg
      361 gccaacagta taccctctac atcccagatc ggaaatgaaa cgacatcaac gccaaacgtt
      421 ttaagagatt tcactgcttt atacacagca taacaccacc ctgccaagcc cgaaggaatt
      481 gcacgacgtg ccgccatcag ccgcccttct tccgcatgat cttctgtcac agcatgtatc
      541 caataaccct cttggtaacg ggtatagggt tccttttcag catgtgtcac gacatgaacc
      601 ttatgcccac gatctgcaaa tccacgagcc atcatggatg tccagcgcgc aattccggcc
      661 tctactgcag gcggaaaaga gcgactgaca aaaacaatac gcagcccgtt tttttctttc
      721 atttgtacaa atggcaactc aggcacagaa aaatccaagc cccccatgcc gcccatgccg
      781 ccctgatcag ccatccttct ctctagtgct cgcctgcctc cttcccggat gccaaatagc
      841 agatcatcat ccagcgacac acgatgtgcc tctgtgattt caccgtgctc agacagccat
      901 ttgttatcat tcaaaatctg attttcatac ttgccaatct cctcgctggc acgaacctca
      961 gaaaacgcag cgccatgacg atgaatgaaa taggatttgc tgattgccga cggatagaga
     1021 gttttaggta tacgattttt gctgcgcaca tggctggctg caaactggtg atagaccaat
     1081 gcattgggct cataaacaat ctgataccca cgatctatca tgcgcagaca aacatcagtt
     1141 tcatccaaaa gataggcaaa aacgtgatca aaacctccaa tttcacgcag agctgaaaga
     1201 cgaaaactgg agttcgtgcc cagcaggctt ggataaatag gcgttccagg aaatgacaga
     1261 ggtcgttcat caaagtaatc atgcggaaaa aacgcattgc caaaacgatc acaaactgtc
     1321 tttctgactt gatagcggac acctgtattg tcaatggtga aaccgcctac tccgcctaca
     1381 gatggatcgg catagtggac tgccagacgc ttcaaccact ctggatgcgg gacggcatca
     1441 tcatccatga atgccacgac atcgcccgaa gccatctgaa tgccgatatt tcgagacatc
     1501 gaaaggttcg cctcggcaca ctccccgatc ttgatgcgtt ccttccagcc ctcaagtatc
     1561 tctcctgttc gatcggtgga aggcccattg atcacaatga cctcataatc cgggtagttc
     1621 aactccgcca acccaagtat ggcatcattg atgtatgcag cccggttata ggtgttgatg
     1681 acaacagaaa atttcaacat tctctaaact ccacgggttt cggccacaaa gcgcagcagg
     1741 tcacgatttg cacgccagaa gtcatcttca tgtatgagct caacgaaaat ggagtgcgcg
     1801 tgctcatgct gcatcatcaa ctcgagtaca actcccacct gcggagacca tcccaaaatc
     1861 aactcatgca gcatgatgac atctgcagac ggtagaagtg agcctgaaga tctcaatcgc
     1921 atttcaagct gagacttgat tccaagtttt tctgcgacaa acagcgcatg ttcttcgaca
     1981 tccagcatcg gcgccccgaa tatcaaggca ttcagataat tgacaagcgc atctgctgct
     2041 ttgccttgct ctttcaatgc gtgcgcgtac tgcacgtaga tgagcggatg tttcgggaac
     2101 aatgtcaatg cacgccaaca gaaatattca gctttggcaa aatcattact gtcgcgcatc
     2161 atatcagcct cggccacaag cccctgaaag tcggtgtctt gcccggcgaa acgcaatgcg
     2221 gctgcgccag cttgacgcgc atccacaatg aactgaatca cggcacgccc aatcagattg
     2281 ggctccatgg ccataccttt ttttcgcatt gcaacacaca tcgaatgaag attggcaacc
     2341 gtatcttcaa gaagaaaacc ttgatcgagc atgccactct cttcaaagac tcgaagacag
     2401 tcaccgacag aacgaacttt ttcgagcaaa agataaactg atttatcttc cttgccaccg
     2461 tttgattgct taactggcct ggctcgtctg ctcaagaatt tcacaccctc ctcctccagt
     2521 catataaagc gtttatagat actagacaac cgttcacaat aagcttcttt tgaaaatttc
     2581 aaaccctgta ttctaccagc cgaggcataa ccttctcgaa tggtatcatc cacatccaaa
     2641 acgcgaatgg cccgggcgat ctcctggctg tcatatgggt ttatcaagat tgcggactct
     2701 ccagctatct cgggtaatga agcggtgttg gcggcaatga ctggagtgcc cagcaacatg
     2761 gattcaagca ccggcaaccc aaagccttca tacagcgacg gaaaaacagt cgccttggca
     2821 cctctgatca ggctgacaag caacggaaaa gggacgtaag aaagccgaat gacctttctc
     2881 ttgatactcg tttcagaccc accccgatcg cgcatagcac cttcgttaag gagtttcaac
     2941 tcctcttctg atttccaggc ttgagcgcca acaaccacca agggtgcctc aacaccactg
     3001 gcgagatagc cttcgatcat acgtccaatg ttttttttgg gctcgatgga gccccagaac
     3061 aggtaataat tcttgtagcc cagtccaaag gtgccctcga tttcgcgctg caccacctcc
     3121 tcgggtttgt cgacatactt ttccggaatg tgaaccgatt ggtaggtgtt ggtgatccgc
     3181 tcttcgggca cgcccagcag ttccatgatg tcccgctttg atgtttcaga aacggtgacg
     3241 atatggtcag ccctcctggc aatggcagac accagcctga agtagcgacg cttgttatca
     3301 agtgtggtgt agggcagacg caagggcacc agatcatgca gggtatagat gtttggaacg
     3361 ccgggcaccc tgagcgcaag cgggtaggtc cagtgcatca gatcaggttt ttgctctggg
     3421 taaattttgt ttaactgctt gaagacacca aaaacattat gtgctttggt aaaaatattg
     3481 ggggagtttt caatatggtc gaaatagggc agacgacttt tgaaggtgtc agaaattacc
     3541 ttgccggtga tcggtaccct agtcgcatta aatccgaatg gcgtgcgaaa gagatccttg
     3601 gctgtgttca acacacgaag aaccgctggg gtcttgcgca ggttgtcatc aaagaaagtg
     3661 atctctttca tcagatccga catgccaggc gcagcacggt tgccatagag aacatccacg
     3721 ccatagccca tttcatgcag gcagtggctg agattgcggg cataggtcga caccccggta
     3781 cctttttcga gcccgaggtt gcggccatca accatgatcg tttttttgct ggacatttct
     3841 ctcaacatct ttcctgataa aaggaatagg cttcttcaat gttggagaac tgatccagtt
     3901 ttccccgatg aagcaccaga gctttaccgc aatgctcccg aatgttgtgc gcttcgtgtg
     3961 acaccagtac aaaagcacgg ctcttgcgct tttcaaacaa ttcatgctgg catttctcat
     4021 gaaaacgggc gtcacccacc gtgatcacct catcgatcag aaagcagtcg aactccaccg
     4081 ccatggaaat ggcaaacg
//
LOCUS       DAOQLC010000242         3934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_11589_length_3934_cov_20.305749, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000242 DAOQLC010000000
VERSION     DAOQLC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11589_length_3934_cov_20.305749"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1965)
                     /locus_tag="PL000_21055"
     CDS             complement(<1..1965)
                     /locus_tag="PL000_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013099797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HNC91428.1"
                     /translation="MTERVGVYVCHCGSNIAGTVDVVDVANWAVEKLGRQGVIIAREY
                     KFMCSSLGQELIEKDIKELGLTRVVVAACSPHLHEKTFRTAAKNAGLNPYLVELASIR
                     EQVSWVHSDKAVATAKAKAVVSGAVSRVIENRPLEEIKVPINPNTLVVGGGIAGIQSA
                     LEIANAGFHVYLVEREPSIGGHMAQFDKTFPTLDCSACILTPRMVEAGTHPNITLLTW
                     SEVTNVAGYVGNFHVTIKKKARFINEELCTGCGICQEKCPKKVIDDVYEAGLGYRKAV
                     YTPFAQAVPNFPVMDKENCTYFIKGTCKACEKFCPTGAIDFNQQDEMLHIDVGNIILA
                     TGYDLFDARRVSNYGYGRLPNVFTSLEFERLSNAAGPTSGNIVLRDGKTVPKSVGIIH
                     CVGSRDKNFNNYCSVICCMQGLKFAHLVHERTGATVYNFYIDMRTAYKAYDEFYQRVL
                     EEGTLFVRGKVAEVTNAARNEREKGRLIIQCEDTLAGKQRRIPVDMVILSSGLQPRKD
                     AKEVGKMFGISCSMDGWFTEKHPKLDPVATMTEGIYIVGCAQGPKDIPSSVAQGAAAS
                     ARVLDKILQKEVALEPIKASVDQALCSGCRICNGLCPFNAITFIEDDKVTSINPALCQ
                     GCGTCVAACPAGAISGTGFSNEQIIAQIDGL"
     gene            complement(1962..2960)
                     /locus_tag="PL000_21060"
     CDS             complement(1962..2960)
                     /locus_tag="PL000_21060"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HNC91429.1"
                     /translation="MNKYLLYPGCSMDSSAKAYMESLNSIVEPLDLHLEEIEDWNCCG
                     ATEYLGINLIPAYSLIARNLALASKQMNGGTRTVVAPCSACYLNLAKADYYMQERPTL
                     GAKVNEALAAGGLEYKAGTLDVRHLIDVLVYDVGLDAIRKAVTHPLSGLKVVPYMGCM
                     LPRPDYQHRWSDHEHPTELDDLLRALGADVIDYPLTTSCCGGHMTQIGPETAFELIHR
                     LVSDADERGAQMMVTVCPMCQMNIDAYQNEMNHFFGTDFHMPILFFTQLMGLAFGKAP
                     EELGIGVELVSSRNALAHIGVEVPVTQEPAAPRKKDEGLPMPRRWTKHEGKKEAVK"
     gene            complement(2957..3553)
                     /locus_tag="PL000_21065"
     CDS             complement(2957..3553)
                     /locus_tag="PL000_21065"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNC91430.1"
                     /translation="MPQMTDERVRAGMTREEDLSLLHEVSMKTAGVSHLEMCIQCGTC
                     GGSCPVAADMDHTPRMLFAMLRAGMREEVLRSNTPWLCVSCYHCVVSCPQEVHIADVM
                     YTLKGMAIHAKLYTDSTAPHFSQTFVDMVETFGRGYELGLATRHYLRHFPLRLPGMMP
                     MGFGMISKGRMAITPKKIKHMDQLTKILDRAKELELTS"
BASE COUNT          917 a         1204 c          901 g          912 t
ORIGIN      
        1 caacccatcg atctgtgcaa tgatctgctc attgctaaag cccgtgccgc tgatcgcacc
       61 cgcgggacaa gccgccacgc acgttccgca gccctgacac agagcgggat tgatcgaggt
      121 cactttgtca tcttcaataa aggtgatggc attgaacgga catagtccat tgcagatgcg
      181 gcagcctgaa cacagcgctt gatctacact ggctttgatc ggctcaagcg caacttcctt
      241 ttgcaggatc ttatccagca cacgcgccga agccgccgcc ccttgcgcca cgctggatgg
      301 aatatccttc ggaccttgcg cacagcccac gatgtaaatg ccttcggtca tcgtggcaac
      361 ggggtcgagt ttgggatgct tctctgtgaa ccaaccatcc attgagcagg aaatgccgaa
      421 catcttgcca acttcttttg catctttgcg cggctgcaat ccgctggaaa ggatgaccat
      481 atccacaggg atgcggcgct gcttgcctgc cagcgtgtct tcgcattgaa tgatgagcct
      541 gcccttttct ctttcattgc gcgccgcatt cgtgacctca gccactttgc cgcgcacgaa
      601 gagagtccct tcttcaagca cgcgttgata gaactcatcg taggctttgt acgccgtacg
      661 catgtcaata taaaagttat agaccgttgc ccccgtgcgc tcgtggacaa gatgcgcgaa
      721 cttcaatccc tgcatgcagc agatcaccga gcaataatta ttgaaattct tatcgcgcga
      781 ccccacgcaa tggataatgc ccacgctctt cggcacggtc tttccatcac gcaaaacaat
      841 atttcccgaa gtcggccccg ccgcattgga caagcgctcg aactccaagc tcgtgaacac
      901 attcggcaaa cgtccatagc cgtaattgct cacgcgtcgt gcgtcgaaca ggtcatagcc
      961 agtggcaagg atgatattgc caacatcgat gtgcagcatt tcatcctgct ggttgaaatc
     1021 gatcgcgccc gttgggcaga atttctcgca agccttgcat gtgcctttga taaagtaggt
     1081 gcagttctcc ttatccatca cggggaagtt cggcacggct tgcgcgaacg gcgtgtacac
     1141 cgccttgcga tatccaagcc ccgcttcgta cacatcgtcg atcactttct tcgggcactt
     1201 ctcctgacaa attccgcagc ccgtacaaag ttcttcgttg ataaaacgcg ccttcttttt
     1261 tatcgtcaca tggaagttgc ccacatagcc agccacattc gtcacttcac tccatgtcag
     1321 cagcgtaatg ttcgggtgtg ttcccgcctc caccatgcgt ggagtgagaa tacacgcgga
     1381 acaatccagc gtggggaaag tcttatcgaa ctgcgccatg tgcccgccaa tggacggttc
     1441 acgttccacg agatacacat ggaatcccgc attcgcaatt tcaagcgccg attggattcc
     1501 cgcaatgccg ccgcccacca ccaaagtatt tggattgatc ggaactttga tctcttccag
     1561 cggacggttc tcgatcactc tcgaaaccgc gcctgataca acagccttgg cttttgccgt
     1621 cgctacggct ttatctgaat gtacccacga aacctgctcg cggatcgaag ccaactccac
     1681 caaatacgga ttcaatcccg cattcttcgc cgccgtgcga aacgtctttt catgcaagtg
     1741 cggtgaacac gccgccacga ccacacgcgt caatcccaat tccttaatat ctttctcgat
     1801 caattcctgt cccaaactcg aacacatgaa tttatattca cgcgcaatga tcacaccctg
     1861 ccgcccaagt ttttccaccg cccaatttgc gacgtcaacg acatccacag tgcctgcgat
     1921 gttcgaaccg cagtgacaga catacactcc aactcgctct gtcatttcac cgcctccttc
     1981 ttcccctcat gcttggtcca gcgacgcggc atgggcagcc cctcatcctt ttttcttggc
     2041 gcggcgggtt cctgcgtcac aggcacttcc acgccaatgt gcgcgagtgc gttgcgcgag
     2101 ctgaccagtt ccacgccaat gcccaactct tcaggcgctt tgccgaatgc cagccccatc
     2161 aactgcgtga agaacaggat cggcatgtga aaatccgtgc cgaagaaatg attcatctca
     2221 ttttgatagg catcgatgtt catctggcac atcgggcaga ccgtcaccat catctgcgcg
     2281 ccgcgttcat ccgcatcaga cacaagcctg tggataagtt caaacgcagt ttcaggcccg
     2341 atctgagtca tgtgcccgcc gcagcaactg gtcgtgagcg gataatcgat cacgtccgcg
     2401 cccagtgcgc gcagcaagtc atccaactcc gtgggatgtt catggtctga ccagcggtgt
     2461 tgataatcag gccgcggcag catgcagccc atgtacggca ccaccttgag tccgctcaac
     2521 gggtgggtca ctgcctttcg gatggcatcc agccccacgt cgtagacaag aacgtcgatc
     2581 aggtgacgca catccagcgt gcccgctttg tattccaatc caccagccgc aagcgcctca
     2641 ttcactttcg cgcccaaggt tgggcgttcc tgcatgtagt aatctgcttt ggcaaggttg
     2701 agataacaag ccgaacacgg cgcaacgaca gtgcgtgtac cgccattcat ttgctttgaa
     2761 gccaatgcaa gatttcgcgc gatcaacgaa tacgcgggga tcaagttaat gccgagatac
     2821 tccgtcgcgc cgcagcaatt ccaatcttcg atctcttcca aatgcaggtc aagcggctca
     2881 acgatggaat tgagcgactc catatacgcc ttcgcactgg agtccatgga acagcctgga
     2941 tacaaaagat acttgttcat gaggtcaact ccaattcctt ggcgcgatcc aagatcttcg
     3001 tcaattgatc catgtgtttg attttcttcg gcgtgatcgc catacgcccc ttcgagatca
     3061 tcccaaagcc catcggcatc atgcctggca gccgcagcgg aaaatgccgc agataatggc
     3121 gtgttgccag cccaagttca taaccgcggc caaaggtttc caccatgtcc acaaaggtct
     3181 gcgagaaatg cggcgcggtc gaatcggtgt acagctttgc atggatcgcc atccctttga
     3241 gcgtgtacat cacatccgcg atatgcacct cctgcggaca gctcaccacg cagtgatagc
     3301 acgacacaca tagccacggc gtattgctgc gcagcacttc ctcgcgcatc cctgcccgaa
     3361 gcatggcaaa caacatgcgc ggcgtgtggt ccatatccgc cgccacaggg caggaaccgc
     3421 cgcacgtccc gcattggatg cacatttcca aatgcgaaac gcccgcagtt ttcatgctca
     3481 cttcatgtaa taaagaaagg tcttcttcgc gcgtcatccc cgcgcgcaca cgttcatctg
     3541 tcatctgggg catagtgcct ccgtgctaca actcaaccaa ccagttgctg ctcggcctga
     3601 ccgaagtcag ccattcgcag ttaatgtatg gacatccccc atattcatca tagtatgagg
     3661 gtttgcaaat aacagtaacg aagataatcg tacaatcctg acaaacatcc ctgacaagcg
     3721 atacaatttc taatatgaac atttttgatc tttttgaaaa taatgtgcga aattttccca
     3781 acaaacccgc ctttatctac ttcgataaac gcaagtggat aacgctcact tatcaggata
     3841 tcttcgacaa aacccagcgc ttcattcgtg gacttgaaac tgggcactac accccaggca
     3901 tcaccgccgc cttattaact ccccccagcg cgga
//
LOCUS       DAOQLC010000243         3877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_11913_length_3877_cov_18.690738, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000243 DAOQLC010000000
VERSION     DAOQLC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11913_length_3877_cov_18.690738"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /gene="ltrA"
                     /locus_tag="PL000_21070"
     CDS             complement(<1..849)
                     /gene="ltrA"
                     /locus_tag="PL000_21070"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNC91431.1"
                     /translation="MPNNSQSHSETWELYGENLDENLKDLSVRLKQGAYRAKPVKRVF
                     IAKPDGRQRPLGIPALEDKIVQRSMVQVLNAIYETDFLGFSYGFRPGRNPHNALDALY
                     VGLQTKKVSWVLDADIRGFFDAIDHEWLIKFIEHRIADKRVVHLIQKWLKAGVLEDGA
                     LTQSDLGTVQGGSISPLLANVYLDYVFDLWTHKWRRTGSRGEVLVVRYCDDFIVTFQY
                     KSDAERYLRELKERFQKFKLELHPEKTRLVEFGRFAIDNFKKRGEGKPETFNFLGFTH
                     ICGAKRS"
     gene            complement(861..1439)
                     /locus_tag="PL000_21075"
     CDS             complement(861..1439)
                     /locus_tag="PL000_21075"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC91432.1"
                     /translation="MRAALYARVSTKEQNTLPMQMKAMKALARQRSWTVSMAISETAS
                     GRATRPERETILMAARQRKIDVVVVWKVDRWGRNLADLISTLEELSALGVALVSTTEF
                     FDMSSPAGKAMVGMLSIFSNFERDMLGERIKAGIRDSKKKHGRPRTALKHAARIKKLY
                     QDGMNKSAIARKLGIGRTSVIRILAEEGKIAK"
     gene            1571..3013
                     /locus_tag="PL000_21080"
     CDS             1571..3013
                     /locus_tag="PL000_21080"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HNC91433.1"
                     /translation="MDPTEIAFERYQIIEPILTRKATLAEISRQTKRPVRTLYNWVAA
                     FQEDGLDGLLPKVRSDKGKRRVANDELKQLIQGLFLRTPPVCIKTIFRQVLEVCEKNN
                     WRKPSYDVVCEIIAEIPAPLKTLAHEGLKAYKQAFGLLNRFEADRPNEIWQADHTPLD
                     ILIFNEKGAAQSPWLTIIIDDYSRAIAGYFLGFQPPSSLRIALALRQAIWYKQLPGWN
                     ICGIPEKFYSDQGSDFRSSHIEQVAIELGFEPIQTIPEEPQGKGKCERFFLTINDMFL
                     SRMPGYKKPGSPLPVPVLSLSQLEERFQKWMIENYLKEVHSETNMPPQERWLSFPHIP
                     KMPASEEKLDLLLLTVAKVRFVRRDGIRFSGFRYFDVSLAGYVGEPVVIRYDPRDLST
                     ILVYKQNVLICKAQCAELVGTKPALKDVRQARNHEAKVQRKLLTGLLSTADKHCPSEK
                     PEPAKETPSLSDQPEYPKFKIRRFACDDVE"
     gene            3000..3803
                     /locus_tag="PL000_21085"
     CDS             3000..3803
                     /locus_tag="PL000_21085"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC91434.1"
                     /translation="MMWNESPQPFLMTGQYKRFAEICNRSMRGKSISLIYGRTGLGKT
                     ESALHYTNWREVAPLLPQSSATRKVPRSVIQSGAAIYTPDVAATPKMVQSGISLLRNR
                     FDELVDQAACWHRFDTGEFAPHKYLKLLIVDESDRLKPASLEFIRDLYDRTNLSIVLI
                     GSPGIERRLQRAAYGQLFSRFSLAYRIEPITEKEAENFIALKWAALGLPLKADGVVAA
                     AIAKLAAGNFRTLHRIFAELQRLQKLNRFQMITPDVLDAARKGLLLVDN"
BASE COUNT         1020 a          891 c          924 g         1042 t
ORIGIN      
        1 gcttcttttg gctccacaga tatgcgtgaa ccccaagaag ttgaacgtct ctggtttacc
       61 ctcgccgcgc tttttgaaat tgtcgattgc gaatcttcca aattcaacta gcctcgtttt
      121 ctctgggtgc agctctagtt tgaacttctg gaatctctcc tttagttctc gtagataccg
      181 ctcagcatcc gacttgtact ggaacgtcac aatgaagtca tcacaatatc gcacgacgag
      241 gacttcgccg cgtgagccag tgcgtctcca tttatgagtc cagaggtcga acacatagtc
      301 tagatagaca ttcgccagta atgggcttat actgccacct tggacggtgc ctaagtcact
      361 ttgcgtcaac gccccatctt ctaacacgcc ggctttcagc catttctgga tgagatgcac
      421 gacgcgttta tcagctatcc ggtgctcgat gaatttaatt agccactcgt ggtctatggc
      481 atcaaagaag ccacgaatgt ctgcatcgag tacccagctc actttcttcg tttgtagtcc
      541 gacgtagagc gcatccagcg cattatgcgg gttgcgtcca ggtcggaatc catacgagaa
      601 tccgaggaaa tcagtttcgt agatggcatt caacacctgt accatggacc tttggacaat
      661 cttgtcttcc agtgccggta tgcctagtgg tctctgtcga ccatctggct tggctatgaa
      721 aaccctcttt actggttttg ctcggtatgc accctgcttg agtcttacag agaggtcctt
      781 cagattttcg tcgagattct ctccgtatag ctcccacgtt tcactgtgac tttgcgaatt
      841 atttggcaaa aattggcgaa ttatttggca atttttcctt cttccgccaa aatacgaatt
      901 acggaagtgc gcccaatccc tagctttctg gcaattgcag acttattcat gccatcttga
      961 tagagtttct ttattcgtgc agcgtgcttt agagccgttc ttggtcgccc gtgttttttc
     1021 tttgaatcac gaatgccagc tttaatacgc tcaccgagca tatcccgctc gaaattagag
     1081 aagatagaaa gcattccaac catagctttg ccagccggag agctcatatc gaagaactct
     1141 gttgtggaga ctagagctac tccaagagcg ctcaattctt ctaaagtaga gatgaggtct
     1201 gcgaggttgc ggccccagcg gtcaaccttc caaacaacga caacgtcaat ttttcgctga
     1261 cgagcagcca tcaatatggt ttctcgctca ggtctggtag ctctgccgga ggcagtttct
     1321 gagattgcca ttgaaacagt ccaggaacgc tgccgggcga gggctttcat cgctttcatc
     1381 tgcatgggca aggtattttg ttcttttgtc gaaacccttg catatagagc agcgcgcatg
     1441 atttatatct ttcaggtgtc caaaaacttt gttagaaaaa ctataaccga agagccgtgg
     1501 gatggggctt ttaatttttt gtttttgggt gtccaataac tatagataat ggacgcttcg
     1561 gagaaaattt ttggacccga cagaaatagc gtttgagcgt tatcaaatta ttgagcctat
     1621 cctaactcgt aaagccactt tagcagagat ttcgaggcaa acaaagagac cggtacgaac
     1681 gctatataac tgggtggctg cttttcaaga agacggcttg gatggacttt tacccaaagt
     1741 cagaagcgat aaggggaagc gtagagttgc caatgacgag ctaaagcagc tcatacaggg
     1801 actattcttg aggacaccgc ctgtttgtat caagaccata ttcaggcaag tcctagaagt
     1861 gtgtgaaaag aataactggc ggaaacctag ctatgatgtg gtttgtgaga ttattgctga
     1921 gataccagct ccactcaaga ctctagctca cgagggcctt aaggcttaca agcaggcatt
     1981 tggactgcta aaccgatttg aggcggacag gccaaatgaa atatggcagg cagatcatac
     2041 acctctggat atcctaatct tcaatgaaaa gggagcggct cagagtccct ggttgacaat
     2101 cataattgat gactatagtc gtgcaatagc tggatatttt ctgggttttc agccaccatc
     2161 gtcattgcga attgcgctag cattgcggca agcaatctgg tataagcagc tcccgggctg
     2221 gaatatttgc ggcataccgg aaaaatttta ttctgatcaa ggtagtgatt ttaggtcatc
     2281 gcatattgaa caggtggcaa tagagcttgg ttttgagcca attcaaacaa taccagaaga
     2341 gcctcaagga aagggaaagt gtgagcgatt tttcctaacg atcaacgaca tgtttctttc
     2401 gcgcatgcca ggctataaga agccgggaag tcctttgcca gtcccagtac tgtcactgtc
     2461 tcagcttgag gaacgatttc aaaaatggat gatagaaaat tatctgaaag aagttcactc
     2521 ggaaacgaac atgcctccac aagagcgctg gctctcgttt ccgcatattc caaagatgcc
     2581 tgcgtccgag gagaaattag acctccttct tctgacggtg gccaaggttc ggtttgtacg
     2641 acgggatggg attaggtttt cagggtttcg atattttgat gtaagtctcg ccggttatgt
     2701 cggagaaccg gtggtcatca gatacgaccc tcgcgatcta tctactattc ttgtttacaa
     2761 gcaaaatgtg ctgatttgca aagctcagtg tgctgagctc gtaggcacaa aacccgctct
     2821 gaaagatgtg aggcaggctc gcaatcatga agcgaaggtt caaaggaaac ttttgactgg
     2881 acttctttcc actgccgata aacactgccc ttccgagaag ccagaaccag cgaaagagac
     2941 cccctctctg tcagaccagc cggagtaccc gaaatttaag ataaggagat tcgcgtgtga
     3001 tgatgtggaa tgagagtccc caaccatttc taatgaccgg gcagtataag aggtttgcag
     3061 agatatgtaa tcgaagcatg cgcggcaagt ctatttctct tatctacggg cgcactggtt
     3121 tgggcaaaac agaaagcgca ttacactaca caaattggag agaagtagcc ccgctacttc
     3181 cacagtcatc ggccactcga aaagttccaa gatcggttat ccagagcggg gcagcaatct
     3241 atactcctga tgttgctgcc acaccaaaga tggtgcagtc tggcataagc ctcttaagaa
     3301 ataggttcga tgagctggtt gatcaagcgg catgctggca tagatttgat accggcgaat
     3361 tcgcacccca taagtatctg aagcttttga tagtcgatga aagtgaccgg ctcaagccgg
     3421 catcgttgga gtttattagg gacttgtatg accgaactaa cttgagtatt gtgctcattg
     3481 gctctccggg aattgagcgt cgattgcaga gagctgccta tggccagcta ttctccaggt
     3541 ttagtcttgc ctatagaatt gaacccataa cggaaaagga agcagaaaat tttatagcgt
     3601 tgaagtgggc tgcattggga ctccctttga aagcggacgg agtagtcgcc gctgctattg
     3661 ccaaactggc agcaggaaac tttcgcactt tgcacagaat cttcgccgaa cttcaaaggc
     3721 tacagaagct gaatcggttc cagatgatta ctccagatgt ccttgatgct gccaggaaag
     3781 ggcttttact ggttgataac tgaaaattgc caaataattc gccaattttt gccaaataat
     3841 tcgccacaaa gtctgccaaa taattcgcaa agtcaca
//
LOCUS       DAOQLC010000244         3853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12040_length_3853_cov_13.042654, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000244 DAOQLC010000000
VERSION     DAOQLC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_3853_cov_13.042654"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1150)
                     /locus_tag="PL000_21090"
     CDS             complement(113..1150)
                     /locus_tag="PL000_21090"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC91435.1"
                     /translation="MMQNLPSTDLGHILDHTRNLWEDLRGARLFITGGTGFFGTWLLE
                     SFLFANRQLDLKASASVLSRKPASFQKAFPHLGLDPAITLIKGNARDFKFPRGNFSHI
                     IHAATETNPDFTDVDPLEKFSSNIEGTRHVLDFAVKSGAEKFLFTSSGAVYGKQPAEI
                     THIPETYLGAPEPTDTNSAYGQSKRASEFLCAAYAKQHGFDAAIARCFAFVGPRLPLN
                     SNYAIGNFIRDAMSSTSIQIGGDGTPYRSYLYAADLAIWLWTILLRGQSCHPYNVGSE
                     NDLIILQLAKTVADVIAPELPIQVAKRPTPNKTAERYVPSTQRAKTELGLQEFTKLPD
                     AIQRTAQWYAL"
     gene            complement(1125..2444)
                     /gene="rfbH"
                     /locus_tag="PL000_21095"
     CDS             complement(1125..2444)
                     /gene="rfbH"
                     /locus_tag="PL000_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HNC91436.1"
                     /translation="MSERSEQLRAKILELTVEYAAEEFPERKFVPGVSSVPVAGRVFD
                     SADVQSLVDSSLDFWLTEGRFARQFEKDFARFFNMRHSILVNSGSSANLLALTCLTSP
                     LLKERQLKPGDEVITVATGFPTTVNPIFQNNLVPVFVDVDIPTYNIDVNQLEAALSQK
                     TRAVMIAHTLGNPFNLEVVTAFCKQHNLWLVEDCCDAVGATYANRMVATFGDVATVSF
                     YPAHHITMGEGGALLTDKPKLKKIIESFRDWGRDCWCEPGKDNTCGKRFDWQLGDLPQ
                     GYDHKYTYSHIGYNLKATDMQAAVGVSQIKKLNGFIEKRRENFNYLKEQLSPLQDHFV
                     LPEATQNSNPSWFGFPIFVRENKNFTRDAILRSLEARKIGTRLLFGGNLIRQPAYRNQ
                     NYRIIGDLKKSDEVMHNVFWIGVYPGLTRPMLDYVVEALHDAKSSLH"
     gene            complement(2437..3432)
                     /locus_tag="PL000_21100"
     CDS             complement(2437..3432)
                     /locus_tag="PL000_21100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNC91437.1"
                     /translation="METMELNKAFWLDRPTFVTGGTGLVGSWLVKRLLAAGADVVCLV
                     RDWVPQSEMVRAGYIEKVKVVRGDIRDRDMLERTLGEFEIDTVIHLAAQTIVTIANRN
                     PVSTFETNIAGTWNILEAARRSPKVKQVVMASSDKAYGDQEILPYDEKTPLQGQHPYD
                     VSKSAADLIAHTYAKSFDLPVAITRCGNFYGGGDLNWNRIIPGTIRSILRGQRPIIRS
                     DGEYIRDYFYVEDGAAAYMLLAEQLATRKELKGEAFNFSNEIQVSVREIVDHVLELMN
                     SDLKPEILNETSNEIRHQYLSAEKARRVLNWKPLFNLDDALALTINWYKDFFKHE"
     gene            complement(3414..>3853)
                     /locus_tag="PL000_21105"
     CDS             complement(3414..>3853)
                     /locus_tag="PL000_21105"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC91438.1"
                     /translation="RVAEFIGNETFMLTYGDGVSNVNIPNLIDFHKQQKRLVTMTAVR
                     PPARFGQMVIEDHRVVQFKEKPQIGEGWINGGFFVLEPGITQYIQGDHTAWEFESLEH
                     IAADKQLSAYQHEDFWQCMDTVRDVHNLEKLWQEGNAPWKLWN"
BASE COUNT          936 a          873 c         1011 g         1033 t
ORIGIN      
        1 catgtgcata attccaccgc ccgaaacgag gaaaatatcg cggatacctt tttcaaccag
       61 gaatttgaca atgtaatcgg aaactttgat catgaatact ctccttgttt aattataaag
      121 cgtaccattg tgcggtgcgt tggattgcat ccggtaattt tgtaaactct tgcaggccca
      181 attcggtctt tgcgcgttgg gttgatggaa catatcgttc agcggttttg ttgggggtgg
      241 ggcgtttggc aacctggatg ggcagctcag gggcgatgac atcagcaacc gtttttgcca
      301 attgaagaat gatgaggtcg ttttctgagc cgacattgta agggtgacag gattgtccgc
      361 gcaggaggat cgtccatagc cagatggcaa ggtcagctgc gtatagatat gaacggtatg
      421 gggtgccatc accgccgatt tgaatggagg tactgctcat cgcatcgcgg atgaaatttc
      481 cgatggcata gttgctgttt aacggcaggc gcggaccgac aaatgcgaag cagcgagcaa
      541 tcgcagcgtc gaatccatgt tgcttggcgt aggcggcgca caaaaattca gaggcacgtt
      601 tgctctgtcc ataggcggaa tttgtgtcgg ttggttcggg tgcgccaaga taggtttcag
      661 ggatatgggt gatctcggcg ggttgttttc cgtacactgc gcctgaactt gtgaatagga
      721 atttttctgc gcccgatttg acggcgaaat ccagcacatg acgagtccct tcgatgttgg
      781 atgaaaattt ttccaatggg tcaacgtctg taaaatctgg atttgtttcc gtggcggcgt
      841 ggatgatgtg cgagaaattt ccgcggggga atttgaaatc acgtgcatta cctttgatga
      901 gggtaatggc agggtcaagc ccaaggtgag gaaatgcttt ttggaaggaa gcaggtttac
      961 ggctaaggac cgatgcggat gcttttaaat ccaattgacg atttgcaaaa agaaaacttt
     1021 cgagcagcca tgtcccaaag aatcccgttc cgcctgtgat gaaaagacgc gcaccacgca
     1081 ggtcttccca caggttgcgg gtatgatcga ggatgtgacc aagatcagtg gagggaagat
     1141 tttgcatcat gaagggcttc aactacataa tcgagcatgg ggcgggtcag tccggggtaa
     1201 acaccgatcc aaaaaacatt gtgcatcacc tcgtctgatt ttttcagatc gccgatgata
     1261 cgataatttt ggtttcgata ggcaggctgg cgaatcaggt ttccgccaaa caggagcctt
     1321 gtgccgatct tccgtgcttc gagcgagcgt agaattgcat cacgtgtgaa gtttttgttt
     1381 tcgcgaacaa agatcggaaa accaaaccaa cttggatttg agttttgtgt tgcctctggc
     1441 agaacgaaat ggtcttgcag cgggcttaac tgttctttga gatagttgaa attctcgcgc
     1501 cttttttcga taaagccatt tagttttttg atctgcgaaa cgcccacggc agcttgcatg
     1561 tctgtagctt tgaggttgta gccgatgtgt gagtaggtat atttgtgatc gtagccttgc
     1621 ggcagatcgc cgagctgcca atcaaagcgt ttgccgcagg tattgtcttt gcctggctca
     1681 caccagcaat cgcggcccca atcgcggaat gattcaatga tctttttcaa tttgggtttg
     1741 tcggtgagta atgcgccgcc ttcacccatg gtaatgtgat gtgctggata aaaactaacg
     1801 gtcgctacat cgccgaaagt tgccaccatt cgattggcat aggttgcgcc gacggcatca
     1861 cagcaatctt ccaccaacca gaggttgtgc tgcttacaaa aggcagtcac tacttcaagg
     1921 ttgaaaggat tgccaagtgt gtgggcgatc attacggcac gggttttttg cgaaagcgcg
     1981 gcttcaagtt gatttacgtc aatgttgtag gtgggaatat ccacatccac aaaaactgga
     2041 acgaggttat tttgaaagat cgggttgacc gttgtcggaa accccgttgc cacagttatg
     2101 acttcgtcac ctggtttcaa ttgcctttct ttcaacaatg gagaagtaag gcaggtgagt
     2161 gccagtaaat ttgccgacga acctgaattc accaaaatgg aatggcgcat gttgaagaag
     2221 cgtgcaaaat ccttttcgaa ttgccgcgca aaacggcctt ctgttaacca gaaatcaagg
     2281 ctggaatcga ccaatgattg cacgtcggct gaatcgaata cgcgtcccgc cacaggcacg
     2341 gaagataccc ctgggacgaa tttgcgttcg gggaattctt cggcggcata ttcgaccgtt
     2401 aattccagaa tttttgcgcg cagttgctcg gatctctcac tcatgtttga aaaaatcctt
     2461 gtaccagtta atggttaatg ccagcgcgtc atcaaggttg aaaagcggtt tccaattcaa
     2521 tacgcggcgg gctttttctg cgctaaggta ttgatgacgg atctcgttac tggtctcatt
     2581 gaggatttca ggtttaagat cggaattcat caactctagg acatgatcca cgatctcgcg
     2641 cactgatact tgaatctcgt tcgaaaagtt gaaggcttca cctttgagtt ccttgcgggt
     2701 ggcgagttgt tcagccagca acatgtaggc tgccgcgcca tcttccacgt aaaaataatc
     2761 gcggatgtat tcgccgtcgg agcggatgat cggacgctgt ccgcgcaaaa tggagcggat
     2821 cgttcctgga ataatacgat tccagttcaa gtcaccgccg ccatagaagt ttccacagcg
     2881 ggttatggca acaggcaggt cgaaactttt tgcataagta tgggcgatca ggtctgcagc
     2941 tgatttgctg acatcatatg gatgttgtcc ttgcagcggg gtcttttcat cgtacggcag
     3001 aatctcctga tcgccatagg ccttgtcgga ggatgccatg acaacctgct ttacttttgg
     3061 gctgcgtcgg gctgcttcga gaatattcca tgtgccagca atattggtct caaatgtgga
     3121 gaccgggttg cgattcgcaa tggttacgat ggtctgagca gcaaggtgga tgacggtgtc
     3181 aatttcaaat tcgccgagtg tgcgttcgag catatcgcgg tcacgaatat ccccgcggac
     3241 aacttttacc ttctcaatat aacctgcacg gaccatttca ctttgcggca cccaatcgcg
     3301 gacaaggcag actacatctg ctcctgcggc aagaagcctt tttaccagcc atgacccaac
     3361 caggcctgtg cctcctgtga caaatgtggg gcgatccagc caaaaggctt tgtttaattc
     3421 catagtttcc atggggcatt accttcctgc cagagctttt cgagattatg aacgtcgcga
     3481 acggtgtcca tacattgcca aaagtcttca tgctgatagg ctgaaagctg cttgtcagcc
     3541 gcaatatgct ccaaagactc aaattcccag gctgtgtggt cgccttgaat gtattgggta
     3601 ataccaggct ccaaaacaaa aaaaccgccg ttgatccaac cttcaccgat ttgcggtttt
     3661 tccttgaatt ggacgacgcg gtgatcttcg ataaccattt gtccaaaacg tgcaggcgga
     3721 cgaactgcag tcattgtgac cagccttttt tgctgcttgt gaaaatctat caggtttgga
     3781 atattcacat tgctgacacc atcaccatag gtgagcatga aggtctcgtt gccaatgaac
     3841 tctgccacac gtt
//
LOCUS       DAOQLC010000245         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12055_length_3850_cov_22.853491, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000245 DAOQLC010000000
VERSION     DAOQLC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12055_length_3850_cov_22.853491"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PL000_21110"
     CDS             complement(<1..267)
                     /locus_tag="PL000_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91439.1"
                     /translation="MKTLKLTRKDFKGTDSYWKEYVGKEDVSVYEGNIEIEGGLGYVR
                     FASLKATGFVLSGAGTGIEAGEGIKSGWGIEAGMGIKAGMGIEAG"
     gene            complement(300..683)
                     /locus_tag="PL000_21115"
     CDS             complement(300..683)
                     /locus_tag="PL000_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91440.1"
                     /translation="MKEKDIQVLFARINRPSRSEVYELKITKSGRLPFSRLAKHQECS
                     LLNAETTGLYHKISDMSAGRKPFDSFFLAGVPAYVVACFYNPRKPKILYFIRIKDFID
                     IRDHSEMKSMTEEEAKVICEKTIVV"
     gene            complement(1065..1769)
                     /locus_tag="PL000_21120"
     CDS             complement(1065..1769)
                     /locus_tag="PL000_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91441.1"
                     /translation="MALHEYDGAGKTYLSIVDGKFRQTVGKGSNRPEGIPEKAVRRDW
                     KAPDGTEGTKWEMVYNAVDGTIVDMYIKDSEYGKFLNVEFDGGEVIQMHTDHRYFGDF
                     VKKIASCDINKPVFVIPYDFEDPKNQDKRIVGVKIVQTGHGWEKDSAPDFFYDFEKKK
                     NINGFPKSGHTKEKPYDKDDWKEYFLGVKKFLIAYLTLSIIPNIKVKEAPVEEKETIN
                     LEDPGEEKPKLEDVPF"
     gene            complement(1774..2616)
                     /locus_tag="PL000_21125"
     CDS             complement(1774..2616)
                     /locus_tag="PL000_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91442.1"
                     /translation="MQKIIEEVAPGVIRVTTDDSRFYGFDRKDPLTGLPIKKWKASIT
                     WISSFLVKDTNLLRWMANKGYDEAQAYMGQKGARGSMIHAGCEQLVLTGSVKMDDIFI
                     DKDTNEARTLTPDEYENVMFYRDWLLSEKPEILFVEKTVEDSDDALGIAGTVDLGIIR
                     KGKKQIIDIKSGNHYMEHDMQVSAYRYCLGEEWKDADMIALHLGRNLNKKKGYRETVL
                     EDRFDLFLAVRKSWEAKTKERGPLQRDFPLAITLTDEEMKMYTPNLKVDGEKKSKKAN
                     TSKA"
     gene            complement(2634..3206)
                     /locus_tag="PL000_21130"
     CDS             complement(2634..3206)
                     /locus_tag="PL000_21130"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNC91443.1"
                     /translation="MFKKWPNRKYCSRKCLGIAQRKEEKQCLSCGISYVGSGKKFCSK
                     KCQSSFMVKERSPTWKGGINKEKDRRKSFEGVKWRKAVFERDDYTCRMCGKKGGFIEA
                     DHIKPFCDYPELRYELSNGRTLCRGCHRGTDTYGRKRRFIEYNGVRMCLSDWSKAIGI
                     KRGTLDVRIRKLKWSIEKSLTTPWNKSIKP"
     gene            complement(3483..>3850)
                     /locus_tag="PL000_21135"
     CDS             complement(3483..>3850)
                     /locus_tag="PL000_21135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC91444.1"
                     /translation="TKIRRVFSENPDEAVAIARAESGLNPKAESTTDRMADGRAFSHG
                     LFQINLTVSTVGGVKCHEAFEGRNYKAKVIDEALFDKCVRLAQDVDESIKVAKAKYEG
                     RGNWTAWGAYTSGAYLRHL"
BASE COUNT          791 a         1044 c          784 g         1231 t
ORIGIN      
        1 gccagcctcg atgcccatgc cagccttaat acccatgcca gcctcgatgc cccagccaga
       61 cttgatgccc tcgccagcct cgatgcccgt tccagcgccg gagagtacga atcccgtcgc
      121 cttcaaagaa gcgaatcgta catacccgag gccaccttcg atttcgatat ttccctcata
      181 cacggaaaca tcttctttcc caacgtactc tttccagtag ctgtcggtgc ccttgaaatc
      241 ctttctggtg agtttgagcg ttttcataaa aaattctgaa agaatttata tttttgttgt
      301 catactacta tcgttttctc gcaaataacc ttagcttctt cttcagtcat gctcttcatc
      361 tcagaatgat ctcggatatc gatgaagtct tttattcgga tgaagtagag tatcttcggt
      421 ttcctgggat tatagaaaca ggctacgacg tatgctggaa cacctgctag aaaaaagctg
      481 tcgaatggct tcctaccagc gctcatgtcg cttattttat gatagagacc tgtcgtttcc
      541 gcattgagga gcgagcattc ctggtgtttt gccagtcttg agaacgggag tctaccggac
      601 tttgttattt tcagctcgta tacttcggac cgtgatggtc ggtttattct ggcgaacagc
      661 acctgtatat ccttctcttt catactctat ctccttaaaa gcctttatta tacctttcgc
      721 tcgcgcgccg agtttcgccg atgtccgtcc tcctcgtgat cggtgcttcc cgtagtaggc
      781 gtgtcgggat ctggttttcc ctgatgcgtt catatatcgt accgatcggc gacttccctt
      841 cgtctgttcc acctctgttt cacctcgatg taccgttttt tacgctccga ctttatcttc
      901 gcgttttcat agccttttat catagctgaa aaaatacgct ccttcatggc tccgatgtcc
      961 ggggcgacgt attgctcgta ctcatctttc atttcttcgt cggtcatatt ttttggtcgt
     1021 tcaggtttcg gaggggagtt cgagactccc ctccgtcagc ctccttagaa cggaacgtct
     1081 tcaagcttcg gcttttcctc tcccggatct tcaaggttga tggtctcctt ttcttcaaca
     1141 ggcgcttcct tcaccttgat gttcggaatg atggaaagcg taagatacgc gatgaggaat
     1201 ttcttgactc cgaggaaata ttccttccag tcgtccttat cgtacggttt ctccttcgta
     1261 tgaccggact tcggaaatcc gttgatattc ttcttctttt cgaagtcgta gaagaaatcc
     1321 ggggcgctgt ccttctccca tccgtgccca gtctggacga tcttcactcc gacgatccgc
     1381 ttatcttggt tcttcggatc ctcgaaatcg tacggaatga cgaagaccgg cttattgatg
     1441 tcgcatgacg cgatcttctt cacgaaatct ccgaagtacc gatgatcggt gtgcatctgg
     1501 atgacttccc ctccgtcgaa ctcgacattg aggaatttcc cgtattcgga atccttgatg
     1561 tacatatcga cgatcgttcc gtctacggca ttgtagacca tttcccattt cgtcccttcg
     1621 gtcccgtccg gagccttcca gtctctacgg accgccttct ccggtatacc ttcaggacga
     1681 ttcgatccct tgccgaccgt ctgtctgaac ttcccatcga cgatagaaag atacgtcttc
     1741 cctgctccat cgtattcatg caatgccata ttgttatgct ttagatgtgt tagctttctt
     1801 gcttttcttc tccccatcga ccttgaggtt cggtgtatac atcttcattt cttcgtccgt
     1861 gagcgttatg gcgagcggga agtcacgctg gagcggtcca cgctccttcg ttttcgcctc
     1921 ccatgatttt cgtacggcga ggaagagatc gaatcggtct tcgagcaccg tctcccggta
     1981 tcctttcttc ttattgaggt tgcgtccgag atgaagcgct atcatgtcgg catccttcca
     2041 ttcctctccg aggcagtacc ggtatgctga cacctgcata tcgtgctcca tgtaatgatt
     2101 gccggacttg atgtcgatga tctgtttctt tcctttccgg atgataccga ggtccacggt
     2161 cccggcgatg ccgagcgcat cgtcggaatc ctctactgtt ttttcaacaa aaagtatttc
     2221 cggcttttcg gaaaggagcc agtccctgta gaacatgacg ttctcgtatt catccggtgt
     2281 aagcgtccgg gcttcatttg tgtctttatc gatgaagatg tcgtccatct ttaccgatcc
     2341 ggtaaggacg agctgctcgc acccggcatg gatcatcgag cctctagcgc ctttttgtcc
     2401 catgtacgcc tgcgcctcgt cgtacccttt attcgccatc cacctgagaa ggtttgtatc
     2461 tttcacgagg aaacttgata tccaggtgat cgacgccttc catttcttta tcgggagtcc
     2521 ggtcaacggg tctttccggt cgaatccata aaatctgctg tcgtcggtcg ttacccggat
     2581 gactccggga gcgacttctt caataatctt ttgcatgatg gtgctttcgt gaattatggc
     2641 tttatagatt tattccatgg tgtggttaac gatttttcta tactccattt taatttcctt
     2701 atccttacat caagagttcc tctttttata ccgattgctt ttgaccagtc agacaggcac
     2761 attcttaccc cattatattc aataaatctt cgttttctac catatgtgtc tgttccccta
     2821 tgacatcctc tgcaaagtgt cctgccatta cttaactcat accgtaattc tggataatcg
     2881 caaaatggtt ttatatggtc tgcttcgatg aaacctcctt ttttaccaca cattctgcaa
     2941 gtataatcgt ctctctcaaa aactgctttt ctccatttta ctccctcaaa agactttctc
     3001 cgatcttttt ccttatttat ccctcctttc catgttgggg atcgttcttt aaccataaaa
     3061 gaactttggc attttttact acagaatttt ttaccgctcc ctacatatga aattccgcaa
     3121 gacaaacatt gtttttcctc ttttcgttgt gctattccta agcattttct tgagcaatat
     3181 tttctgttag gccatttctt gaacatgatt ccacaactag ggcataccaa gttttgtgtt
     3241 ggcaggtgtg gtggtggaga tttcggcatt tttcctttta ggctatccca ataacattca
     3301 ctagcacaaa atttttttct tctatttata tgtaggaatt ccttattaca attcttgcaa
     3361 atcactttta ccataaaaca aagtccgctc aacctgttgg gcaagcggag cggactttgc
     3421 tcaccagttg cccaacaact gatgaattaa ttaccatcag tatatcagat tcgcgaatat
     3481 tgtcaaagat gtcgaagata tgccccagac gtatacgccc cccaagccgt ccaattcccg
     3541 cgtccctcgt acttcgcctt tgcaaccttg atgctttcgt ccacgtcctg cgcgagccgg
     3601 acacacttat cgaacaacgc ttcgtcgatg accttcgctt tgtagttccg tccctcgaac
     3661 gcctcgtgac acttcactcc gccgaccgtc gagaccgtca ggttgatctg gaagaggccg
     3721 tgcgagaacg cccgtccatc cgccatccgg tcagtcgtcg attccgcttt cggattgagc
     3781 ccagactccg cacgagctat cgcgacagcc tcatcaggat tctccgagaa gacgcgtcgg
     3841 atcttggttt
//
LOCUS       DAOQLC010000246         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12059_length_3849_cov_27.060095, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000246 DAOQLC010000000
VERSION     DAOQLC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12059_length_3849_cov_27.060095"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PL000_21140"
     CDS             <1..422
                     /locus_tag="PL000_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91445.1"
                     /translation="KHFEVIIRKMLSKVQVTRPGDTKYLPGDAVDRLEIRKINEALVA
                     EGKQPARFQEILLGVTKASLSTDSFLSASSFQHTIKVLASAAIGSTTDPLFGLKENVI
                     IGKLIPAGTGFIHGRFADAESTQNATQWSNMPDMGSI"
     gene            665..1996
                     /gene="ablA"
                     /locus_tag="PL000_21145"
     CDS             665..1996
                     /gene="ablA"
                     /locus_tag="PL000_21145"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNC91446.1"
                     /translation="MPTPIVSKRAPIYADVPDEKWNDWRWQLSHRLNTAEDIEKVIPL
                     TDSERKALQTQGLFRVDVTPYFISLIDPNDPNDPTRKQIIPVSEELNAFTAMMEDSLA
                     EDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPGQTFNRQEFEMQIEYL
                     KKTPQVRDVLLSGGDPLVLAPKVLEELLTRLREIPHIEIVRIGSRVPVFMPMRITQEL
                     CDMLAKFHPLWLNIHVNHPNEISLELAQACDRLTRAGIPLGNQSVLLAGVNDCAHLQR
                     ELVQKLTRIRVRPYYLYQCDLVEGAGHFRTPVAKGIEIIEALRGHTSGYAVPQFIVDA
                     PGGGGKIPVMPNYMLSMSDHKVIVRNYEGYVTTYEEPTEYKSHDPKTCKFCQSKRPEL
                     GQTGLTGLLDGEELFIKPEGFDELHNRGGGQHRLRDENKWKPLGIGSGETD"
     gene            2030..2677
                     /locus_tag="PL000_21150"
     CDS             2030..2677
                     /locus_tag="PL000_21150"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC91447.1"
                     /translation="MKKMNRAMITLLVLALSLSACLPATASNQQAQQVQNTQQSSQDV
                     QSNIETAVAQTVEAQNEISTSVAQTMQAQATLTPTPTATLFSLPTVTPFVVSTPTVRP
                     AGGGGAPVKAEYSCDIIHQRPYDNQEFLHGQEFDIKWTILNTGTKTWPAGYDLKYWSG
                     PKMTTAGTVQLPSMAPGDQFDVVFDAVAPDEPGFQVMTWVVEGQICFPYIAIFVK"
     gene            2697..3326
                     /locus_tag="PL000_21155"
     CDS             2697..3326
                     /locus_tag="PL000_21155"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HNC91448.1"
                     /translation="MLKRNQRIQAILFATVLMLACAPLATPYAPPTFDLLQVNTIIAE
                     TAGAAATQTFVLLPTSTVTPTFTRAPSETPSPTNTFIFVLATPTVPSLTPTPEKSDEP
                     YDCQVLSQNPVDDTSFVPGLAFQTRWVVKNIGTETWGVNTADYRFVSGTKFHKLSVYD
                     MPTSIPPGEKVEIFVEMWAPMDPGTYTTTWRIKVGKTEFCSMKLKIIVP"
     gene            complement(3377..3676)
                     /locus_tag="PL000_21160"
     CDS             complement(3377..3676)
                     /locus_tag="PL000_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91449.1"
                     /translation="MNNKNTGMIATIATALICGCCALFNCAMSIGTFSGNGSFSLGNN
                     SQPMPPAMGAVFLCLSFIMILIPVAVGFFTLRKKPEAAAVVEETPAKDEPLPPAS"
BASE COUNT          919 a         1040 c          953 g          937 t
ORIGIN      
        1 acaagcactt tgaggtcatc atccgcaaga tgttgagcaa ggtgcaggtc acccgtcccg
       61 gtgataccaa gtacctgccc ggtgatgccg tggaccgcct tgagatccgc aagattaacg
      121 aggctttggt tgctgaaggc aagcagcccg cccgcttcca ggagattttg cttggtgtga
      181 cgaaggcttc acttagcacc gattccttcc tctcggcctc ctccttccag catactatca
      241 aggttctggc cagtgccgcc attggctcca ccaccgaccc gctctttggc ctcaaggaaa
      301 acgtgatcat cggcaaactc atccctgccg gtaccggctt tatccatggc cgttttgccg
      361 acgcggaaag cacccagaat gccacgcagt ggtcgaacat gccggatatg ggcagcattt
      421 agctgttgtt tttgaaaaga cctgagcccg cccgcgaaaa gtttcgcggg cgggtctttt
      481 ttaaagcaaa aatgggcctg ttgtaaaata ccgacattga cccactttcc gtttgctttt
      541 acaatgttaa tcatgctttt gaagtttgca ggattctttc gtccggacca tgaccctcca
      601 cacacggcag tgtgatctta gtcgtattgt cgtttaaaac ttattttaaa tttctggagg
      661 aaaaatgccc acccctatcg tctcgaaacg tgccccaatc tatgccgatg tccctgatga
      721 aaagtggaat gactggcgct ggcaattgag ccatcgcctg aacactgccg aggatattga
      781 aaaagtcatt ccgcttaccg actcggaacg caaagccctg caaacccagg gattgttccg
      841 tgtggatgtg actccctact ttatttcctt gattgacccc aacgacccga acgatcccac
      901 ccgcaaacag atcatccctg tttcggaaga attgaatgcc tttaccgcca tgatggagga
      961 ttcacttgcg gaggatcgcc actcacccgt ccccgggctg gttcatcggt atcctgacag
     1021 agtccttatg ctggtaacga cccaatgcgc ttcgtactgc cgttactgca cccgttcccg
     1081 catcgttgga gaccccggtc agaccttcaa ccgtcaggaa tttgaaatgc aaatcgaata
     1141 cctgaagaag actccccagg tgcgtgatgt gttgctttca ggcggtgatc cactcgtatt
     1201 ggcgccgaag gttctggaag aactgctcac ccgtttacgc gagattccgc atattgagat
     1261 cgttcgtatt ggctctcggg tgcctgtttt catgccgatg cgcattaccc aggaattatg
     1321 cgatatgctc gccaaattcc accccttgtg gcttaacatc catgtcaatc accccaatga
     1381 gatttcgctg gaacttgccc aggcttgtga tcgtctaacc cgtgcaggta ttccgcttgg
     1441 aaaccagtcc gtccttttgg cgggcgtgaa cgattgtgct cacctgcagc gcgaactcgt
     1501 tcagaagctg acccgtatcc gtgttcgccc atactacctg tatcagtgtg accttgtaga
     1561 aggtgccggt cacttccgta cccctgttgc caagggtatc gagattattg aagccctgcg
     1621 tggacatacc tccggttacg ctgttccaca gttcatcgtg gatgcgcccg gcggtggtgg
     1681 taagattcct gtcatgccga actacatgct gagcatgtct gaccataagg tcattgtccg
     1741 caactacgaa ggctatgtca ccacctacga agagccgacc gaatacaagt cgcatgaccc
     1801 gaagacctgc aaattctgcc agagcaagcg ccctgaactc gggcagactg ggctgacagg
     1861 cctgcttgac ggcgaagaat tgttcatcaa gcctgaaggt ttcgacgaat tgcataatcg
     1921 cggcggcggg cagcatcgtt tgcgtgacga aaacaaatgg aagccgcttg gcatcggttc
     1981 cggcgaaaca gactaaagga acccaaaaaa gtttgcgaag gagaaaataa tgaaaaaaat
     2041 gaatcgagcg atgattactc tgttggtgct ggcgttgtct ctcagtgcat gtctgcctgc
     2101 gaccgcatcg aaccagcagg ctcagcaggt acaaaataca cagcagagtt cgcaggatgt
     2161 gcaatcgaat attgaaacgg ctgttgccca gaccgttgag gcacagaacg agatcagcac
     2221 ttctgtagcg cagaccatgc aggcgcaggc cacgctcaca cccaccccca cggctacatt
     2281 gttttccctt cctactgtga cgccgttcgt tgtttcaaca cccactgtcc gcccggctgg
     2341 tggaggcggt gcccccgtga aagcagaata ctcctgcgat attattcacc agcgccccta
     2401 cgacaatcag gaatttctcc acggtcagga gtttgacatc aagtggacca ttcttaacac
     2461 cggcacaaag acctggcctg caggctacga tctcaaatac tggagcggcc ccaagatgac
     2521 cactgcaggg acggtgcagt taccctccat ggcccccggt gaccagtttg atgtggtctt
     2581 tgatgcggtt gcaccggatg aacccggctt ccaggtgatg acctgggtgg tggaaggcca
     2641 gatctgcttc ccctatattg cgattttcgt aaaataaaag ttctttggag gatttcatgc
     2701 taaagaggaa ccagcgaata caggcgatct tatttgccac tgtattaatg cttgcctgtg
     2761 ccccactggc gactccttac gcccctccaa cctttgacct tttgcaggtt aacacgatta
     2821 ttgcggaaac cgcgggagcg gcggcaacac agacctttgt gttgctgcca acttccactg
     2881 tgacccctac ctttacaaga gcgccatcag agactccatc tccgacgaat actttcatct
     2941 ttgtgcttgc aacgccaacc gtaccttcgc tcacacccac tccagagaaa tctgatgaac
     3001 cttatgattg tcaggtgctc tcacaaaatc ctgtggacga tacaagtttt gtacccggtt
     3061 tggcgtttca aacccgctgg gtggttaaaa acatcggaac ggagacctgg ggcgtaaata
     3121 cagcagacta tcgctttgta agcgggacca agttccataa gctgagtgta tatgatatgc
     3181 ccacctccat tccaccgggg gaaaaggtgg aaatctttgt cgaaatgtgg gctcccatgg
     3241 accccggcac ctataccacc acatggcgga tcaaggttgg aaagaccgag ttctgttcca
     3301 tgaaattgaa gattattgtg ccctaaacaa aaaatcctcc gagtgaaaac tcggaggatt
     3361 ttttgttgtc taataattat gaagcggggg gaaggggttc gtccttggcg ggggtttctt
     3421 caacaacagc tgctgcttcg ggtttcttgc gcagggtgaa gaagccgact gccaccggga
     3481 taagaatcat gatgaaagaa aggcaaagaa atacagcacc catcgcgggc ggcatgggct
     3541 ggctgttatt ccccaatgag aaagacccat ttccagaaaa ggtaccaatg ctcattgcgc
     3601 aattgaaaag ggcgcaacag ccacagatca gggcggtggc aatggtggca atcatgccag
     3661 tgttcttgtt gttcatattt ttctcctcgt aagttgatta ttttcacttg cagtatacgc
     3721 tcaacaaaaa cgggatgcaa gcaattgctg catcccgttg gtactagttg atctaacttc
     3781 tcaacttcgc gccgacttct ttttccagcc gtttgacgat cttgttgcgg atggcggcgg
     3841 catcggcgt
//
LOCUS       DAOQLC010000247         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12419_length_3783_cov_14.563036, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000247 DAOQLC010000000
VERSION     DAOQLC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12419_length_3783_cov_14.563036"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /gene="sixA"
                     /locus_tag="PL000_21165"
     CDS             complement(<1..469)
                     /gene="sixA"
                     /locus_tag="PL000_21165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNC91450.1"
                     /translation="MNLYVIRHAIAVEAGNPKYEEDGQRPLTGKGKDKMKKIAQGLWE
                     METQMDLILSSPAVRAMETAKILANRLDIKKSRLIVTEHLEATGYADQLINEIKEKYG
                     DMENIALVGHEPYLSSLVSVLISGKTDSSLTLKKGGVCLLTTDALEYGKCASLN"
     gene            complement(651..1325)
                     /locus_tag="PL000_21170"
                     /pseudo
     CDS             complement(651..1325)
                     /locus_tag="PL000_21170"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
     gene            complement(1329..2861)
                     /locus_tag="PL000_21175"
     CDS             complement(1329..2861)
                     /locus_tag="PL000_21175"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HNC91451.1"
                     /translation="MQKIAAIDIGSNAIRMIVGRITDSWEVELLENIRLPVRLGQDVF
                     TTGKLQETTIQQAVKAFQHFRRVTDDFEVTRVRAIATSAMREASNSYILTDRIQQTAK
                     IEIEVISGDEEARLIHRAVSDAIKLNGKRAVLIDIGGGSVEVTLSENGNIFSTESYDM
                     GTVRLLKKLDGQKEAGLSFSNKPGQPFAVLVREYAKAARKRIELEIGQEKIDLCIGTG
                     GNVEELGKLRQKLLKQSSSEVLRLSELQELIERISGLSIKDRIRKLKLRPDRADVILP
                     AAIVLQLIVCETGVKQIQIPNVGLKDGLLAEIADELARGPQLPKREQVWGSAIRLGNK
                     YGFDAEHSTLTAKLAGQLFDQSRELHHLDEEERLLLEIGALLHDIGHFINTLDHDKHG
                     YYVLKANSLIGLNTRQQEIVANLVRYHRKFPPTLEDIGFKSLPPADRLIVTKLCALLR
                     LADGMDISHTQHVRSVSLTKKKKCWELNIQGRGDLLLERWGLNKRRSLFQEIFGVEME
                     II"
     gene            complement(2874..3554)
                     /gene="phoU"
                     /locus_tag="PL000_21180"
     CDS             complement(2874..3554)
                     /gene="phoU"
                     /locus_tag="PL000_21180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC91452.1"
                     /translation="MMRIEPNREIHNVRDEILVLSSMVEKAMLDSVDALKDHDFQASE
                     TILREDNKINKKRFEMESLIISLIATRQPIAHDLRMLTAMLDMCTELERMGDYAKGLS
                     TINIRSEGLNLPRILLDLHHMAEKSVDMLHRAITSFVEEDIESARAIAYEDNLIDSLY
                     REIYYEAMDVAIEDARNIERVNHVLWAAHNIERFADRVTNICERTVFVVTGSLNELHL
                     QEQNEIGV"
BASE COUNT          877 a          961 c          786 g         1159 t
ORIGIN      
        1 agttcaggct ggcacacttt ccatattcta atgcatccgt tgtaagcagg catacccctc
       61 cttttttcaa ggttagtgaa ctgtctgttt ttcccgagat caacaccgaa accagactgc
      121 tcagataggg ttcatgcccc accagtgcaa tgttttccat atcgccatat ttctctttta
      181 tttcattgat caattgatcg gcatagccgg tggcttccaa atgttcagtt acgatcaacc
      241 tgctcttttt gatgtcaagc ctgttcgcca gtatctttgc cgtctccatg gcccgcactg
      301 ccgggctgct caagatcagg tccatttgtg tttccatctc ccataacccc tgggcgattt
      361 tcttcatctt gtccttcccc tttccggtca gcgggcgctg accgtcctct tcgtattttg
      421 gattccccgc ctccactgcg attgcgtgac gtatgacgta aagattcatt tttatgctcc
      481 tcgttctttg gtttgtcaaa attccaaaaa gtgaaaattt cacctttgtt ttccataaaa
      541 tcaacaacca gcgatttcag cctggcattg aaaaatcgga tcacactttc ggtatcggtc
      601 cggggggaat gttctttcac gtactccaaa aacgaattca gaaaaacctc cgcatcctga
      661 atgtttccca ttgagccctg atatttatgc atggattcaa aatttccact tggaaaaccc
      721 tcaacaagtg ggtgaattat ctcgatcaga tatcgaaatt ttttgaacgc cagcctcaaa
      781 cgatggatac ttgcagcctg cagcggatcc acctgaccgt aagcctgccc ggctaaagca
      841 taggcattat gcgccgcttc gagcagttgc tcgttaaatt gagcatttgc ctgttcttct
      901 ttttcaaggc tctcccgtat tttcccaatc cgcttgctga gttctgaaag cccattttgt
      961 ttgatgcgtt tgtaagccgc ccgcattaat ttggcttccc gctttttcag gaattttcgg
     1021 aacggcttca actctggcat tgtctcgata ttttccgagg tttccacgag catcacctgt
     1081 acatcacgaa gttcattcaa gtcttcaatc attttcttga tgatttgccg tgccttgcgc
     1141 acccgcgggt gcggagcaat cgtttttgcc agccccaaca caaccaacat gcggcgggat
     1201 gccacacgca agtcatgcac tgcctcatcc gtaaactcca tgcggcaggc tttgtgctgg
     1261 gcacggaatt tcttccaacg ctcatcaaaa gcagaaagta aaattgaatc gttttccttg
     1321 gtcatggttt atatgatctc catttcaacc ccgaaaattt cctgaaacag ggatcggcgt
     1381 ttgttcaatc cccagcgttc aagaagcaga tcacccctgc cttgaatatt cagttcccag
     1441 cattttttct ttttagttaa cgacacgctt cttacatgtt gtgtatggct gatgtccatg
     1501 ccgtctgcaa ggcggagcag cgcacataat ttggtgacga tcaatcgatc agcaggaggc
     1561 agcgatttga aaccgatatc ctcaagagtc ggtggaaatt tgcgatgata acgaacaaga
     1621 tttgcgacga tttcctgttg acgggtattc aatccaatca aggaatttgc cttcaaaaca
     1681 taatacccat gtttgtcatg gtcgagtgtg ttgatgaaat gcccaatatc gtgcaggagt
     1741 gcgccgatct ctagaagcag gcgttcctct tcatcaaggt gatgtaattc tcttgattga
     1801 tcaaataatt gtcctgccag tttggctgtc agggtcgaat gttcggcatc gaatccatac
     1861 ttgttaccca gccgtatggc tgagccccat acctgctccc gtttgggtaa ttgcggccct
     1921 ctggcaagtt cgtctgcgat ctcagccagc aggccgtctt tcaaacccac attgggaatt
     1981 tgaatctgtt ttaccccggt ctcgcacaca attaactgga gcacaatcgc cgcaggcagg
     2041 atcacatccg ctctatctgg ccgtaatttt aatttacgaa ttcgatcctt gatgctcagg
     2101 ccgctgatcc tttcgattaa ttcctgcaat tcgctcaatc gtaatacttc actgctgctt
     2161 tgtttgagca gtttttggcg cagtttgccg agctcttcta cgtttccgcc cgtgccaata
     2221 cacagatcaa tcttttcctg gcctatttcg agttcaatcc tttttcgtgc ggcttttgca
     2281 tattcgcgca ccagcactgc aaagggctgg cctggtttat tggaaaatga aagcccggct
     2341 tctttttgtc catccagttt ttttagcagg cgcactgttc ccatgtcgta actttcggtt
     2401 gagaagatat tcccattttc agaaagggtg acttcaacgc tgccgccccc gatgtcgatc
     2461 agaacagccc gcttcccgtt aagtttgatc gcgtctgaaa ccgcgcggtg aatcaaccgc
     2521 gcttcctcat ccccgctgat tacttcaatt tcgatcttcg ctgtttgttg aatgcggtcg
     2581 gtcaaaatat aactattgga cgcttcacgc atggcgctgg tggcgattgc gcgaacacgt
     2641 gttacttcaa aatcgtctgt cacgcgtcga aaatgttgaa atgctttaac agcctgctga
     2701 atggttgttt cctgcaattt tcctgttgta aaaacatcct gtcccaggcg gaccgggagg
     2761 cggatatttt ccaacagttc cacctcccac gagtcagtga ttcgcccaac gatcatgcga
     2821 atggcgtttg acccaatgtc tatcgccgcg attttttgca tggataacga ttcctaaact
     2881 ccgatctcgt tttgttcctg cagatgcaat tcattcaggc ttcctgtaac gacaaaaact
     2941 gtgcgctcac aaatgtttgt cacccgatct gcgaaccgtt caatattgtg cgcggcccac
     3001 aaaacatgat tgacccgctc gatatttctg gcatcctcga tggcaacatc catggcttcg
     3061 taataaatct ctctgtacaa tgagtcgatc aggttatcct catatgcgat ggctctggct
     3121 gattcaatat cttcctctac aaaggatgtg attgccctat gcagcatatc tactgacttt
     3181 tccgccatgt ggtgaaggtc cagcaagatt cgaggcaggt ttaacccttc cgacctgata
     3241 ttgatggtgg atagcccttt ggcataatcg cccatcctct ccaattcggt acacatatcc
     3301 agcatggcgg tgagcatgcg caggtcgtgc gcaatgggct ggcgggtggc aatcaatgag
     3361 atgatcaagc tttccatctc aaacctcttt ttgttgatct tgttatcttc tctaagtata
     3421 gtttcggatg cctgaaaatc gtgatctttt aatgcatcca cagaatccag cattgctttt
     3481 tcaaccatgc tggaaagcac aagtatctca tctctgacat tgtgtatttc tcggtttggc
     3541 tctattcgca tcatgttgtc atccttcaaa gctggtttat ttcattgaaa gcaagtctca
     3601 aaatagaata tctcgaatca attaacacgg ttgaaatgga aggttaatga tatgtaaaca
     3661 accttcagga gtttccgaca ggttgtttat gatttcacac tccctttatc ttttcatttt
     3721 tgtttactaa atgttaactg gctcttaagt cgaaaataaa ggctttgaag tatgatttca
     3781 gta
//
LOCUS       DAOQLC010000248         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12441_length_3779_cov_24.213749, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000248 DAOQLC010000000
VERSION     DAOQLC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12441_length_3779_cov_24.213749"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1152
                     /locus_tag="PL000_21185"
     CDS             4..1152
                     /locus_tag="PL000_21185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC91453.1"
                     /translation="MPMSSPDLTDADRQAVIEVINTPNLSMGKWTPAFEKTFCDLTGA
                     KHAISVNSGTAGLHLCVRAAGIGAGDLVITTPFSFVASSNALLFENAIPVFVDIDPKT
                     GNINPELVADAAKNIEKFLPRKGADKSGKLKAILPVDVFGQPADMDAINAVAKEHGLK
                     VIEDSCEALGATYKGKQAGTLGDFGVFAFYPNKQITTGEGGVIITSDDQSAHFMRALR
                     NQGRAPGDTWLQHTHLGYNYRIDEMSAAMGASQMTRLDEILTKRNQVAAWYEARLSEI
                     PGVETPYIESFTTRMSWFVYVIRFDKRINRDEMAKKLDARGIPVRPYFLPIHLQPYMV
                     EKFGYKEGDFPVTEDLGQRGLAIPFSGVMTEEQVDFVSNIIREEVMKA"
     gene            1182..2090
                     /locus_tag="PL000_21190"
     CDS             1182..2090
                     /locus_tag="PL000_21190"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNC91454.1"
                     /translation="MNIGHFPPPKRRGLIVHGSIIIVLMVIAFIGFINLSRAEVGPQF
                     LVALLVSLFAFAPIPFFIYRGYSLWRADYHMDRDSLAINWGLRVEDIPLSDIEWMRPA
                     DDLTRPLNLPSVRLPGGLLGMRRHPDLGVVEFLASDAKKLLLVATAKRVFVISPEDPA
                     LLMQTFARATELGSLAPVEAKSVYPSFVVTQAWESGLARYLWLTALFLNLGLFIWASL
                     IIPSTPRVALGPQFVGSALETVPSAQLIIFPVASLLLAVMGWVAGLYFYRWDRERILA
                     FIVWGSSTFSSLLFLLAVLFIITTPV"
     gene            2138..2926
                     /locus_tag="PL000_21195"
     CDS             2138..2926
                     /locus_tag="PL000_21195"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HNC91455.1"
                     /translation="MQLLLGFLLALLIAYLAYKARSLNQSGAIAAAFTGTIIFGIGGW
                     EWAVLLLTFFITSSALSRAFKKRKQGLDEKFSKGHERDAGQVFGNGGVATFFAALHFF
                     FPESSLPWVGFAAALAAVNADTWATELGVLNPNPPRMITDLRKIVEKGTSGGISLIGT
                     LASLTGSALIAFLASLLTDNWFLFPLITLSGLAGSLFDSFLGGTVQAMYYCPTDKKET
                     EKHPLHTCGTPTVHTRGWQWLDNDRVNFACGAAGTLVALLLFFI"
     gene            2932..>3779
                     /locus_tag="PL000_21200"
     CDS             2932..>3779
                     /locus_tag="PL000_21200"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HNC91456.1"
                     /translation="MNKPGQLRSYSFILATLRYRKLIAFLTRVMIALRRKKVILIFGM
                     SRSGTSMLGKFLSLGKSSIYMHEPETELMKQHFGPDRHYTQQNFWDFVHSEVPTELKV
                     HTLACISLFAVLKAPIFSKAIVIKPISFVEVIGESSEALTNANIVYICRHPAGRSESI
                     LRQLQHDQGIEEISLEALEQISQDWGRTNKTIKELHRQNPKWLWVYFEKLTGDPVAEF
                     KKLYTQLGLPWNQSIADEIEKSTTGMDGGYYNIQRDSRKQADKWRNSLTTEQIESVRK
                     GTLPFET"
BASE COUNT          852 a         1119 c          915 g          893 t
ORIGIN      
        1 aaaatcccca tgtcatcccc cgatctgacc gatgccgacc gtcaggctgt catcgaagtg
       61 atcaacacgc ccaatttgag catgggcaaa tggactcccg cctttgaaaa gaccttctgt
      121 gacctgacgg gcgcaaagca cgccatctct gtcaactctg gcacggcggg cctgcacctg
      181 tgtgttcgtg cggcagggat cggcgcaggg gatctggtca tcaccacgcc gttttcgttc
      241 gtggcttcat cgaatgcttt gctttttgaa aatgcaattc ccgtttttgt agatattgac
      301 ccgaagactg gaaacatcaa ccccgaattg gtggctgatg cagcgaagaa tattgaaaaa
      361 ttcctgccga gaaaaggcgc ggacaaaagc ggaaaattga aagcaatcct gcctgtggat
      421 gtgttcggtc aacctgccga catggatgcg atcaatgccg tggcgaaaga gcacgggctc
      481 aaagtgatcg aagattcatg cgaagccctg ggcgccacct acaaaggcaa acaggctggc
      541 acgctcggtg atttcggcgt gttcgcgttc tatcccaaca aacagatcac cacaggcgag
      601 ggcggggtca tcatcaccag tgacgaccag tctgcgcatt ttatgcgtgc cctgcgcaat
      661 cagggacgcg cccccggtga tacctggctg caacacaccc acctcggcta caactatcgc
      721 attgatgaaa tgtctgcggc gatgggggct tcgcaaatga cccggcttga tgaaattctc
      781 acgaagcgga atcaggttgc ggcctggtat gaagcgcggc tttctgaaat ccctggcgtt
      841 gaaacgccat acatcgaatc cttcacgacg cgcatgtcgt ggtttgtgta cgtcatccgc
      901 tttgacaaac gaatcaaccg cgacgaaatg gcaaagaaac tcgatgcgcg tggaattccc
      961 gtgcggccat actttttgcc gatccattta caaccgtaca tggtggaaaa atttggctac
     1021 aaggagggcg attttcctgt caccgaagat ctcggccagc gcggtttggc gattcccttc
     1081 agcggtgtga tgaccgaaga acaagtggat tttgtttcga acatcattcg tgaagaggtc
     1141 atgaaggcat aacttcatcc ttcatctttc atcctttccc aatgaacatc ggacatttcc
     1201 ccccacccaa acgccgcggt ttgatcgtgc atggcagcat catcattgta ttgatggtga
     1261 tcgccttcat tgggtttatc aacctttcac gcgctgaagt cggcccgcaa tttttggtcg
     1321 cgctgttggt ttcgctgttc gcctttgcac cgatcccgtt ttttatatat cgcggctatt
     1381 ccctctggcg cgccgactat cacatggatc gcgacagcct cgccatcaat tggggtttgc
     1441 gcgtggaaga tattccgctc agcgatattg agtggatgcg ccccgccgat gacctcaccc
     1501 gcccgctcaa cctgccttcg gttcggctgc ctggcggttt gcttggaatg cgccgtcacc
     1561 ccgacctcgg cgtggtggaa ttcctcgcct cagatgcgaa aaaacttttg ctggttgcca
     1621 cagccaaacg tgtcttcgtc atttcgcccg aagaccccgc cctgctcatg caaacttttg
     1681 cccgcgcaac cgaattggga agcctcgcac ctgttgaagc gaaatcggtg tatccatcct
     1741 ttgtcgtcac gcaggcttgg gaaagcggcc ttgcccgtta cctctggctc accgccttgt
     1801 ttttgaatct ggggttgttc atctgggcca gcctcatcat cccatccaca ccacgtgtgg
     1861 cgcttggccc gcaattcgtc ggcagcgcgc ttgaaaccgt cccttcggca cagctcatca
     1921 tcttccctgt tgccagtctt ttgcttgcag tgatgggttg ggtggcaggt ttatatttct
     1981 atcgctggga ccgtgaacgc atccttgctt ttatcgtgtg gggctccagc acattttcaa
     2041 gcctgctctt tttgctggcc gtgcttttca tcatcacaac tcctgtttga aaaaacgttg
     2101 gaacgtttga acgttccaac gttccaacgt taaacccatg caacttctcc tcggcttcct
     2161 tctcgcactc ctcattgctt acctcgccta caaagcccgc agcttaaacc aaagcggagc
     2221 cattgccgca gcattcaccg gcacgatcat ttttggcatc ggcggctggg aatgggcggt
     2281 tctactgctt acttttttca tcacgtcatc agctctgagc cgcgccttca aaaaacgcaa
     2341 acaaggtctc gacgaaaaat tttccaaagg ccatgaacga gatgcagggc aggtctttgg
     2401 caacggcggc gtagccacct tcttcgcggc gcttcatttc tttttcccag aatcaagcct
     2461 gccctgggtc ggattcgccg ccgcgcttgc cgcggtcaat gccgatacat gggccaccga
     2521 actcggcgta ttgaatccga atccgccgcg catgatcacc gatctacgca aaatcgtgga
     2581 aaaaggcacc tcgggcggaa tctcattaat cggcacgctc gcctcattaa caggatccgc
     2641 cttaattgca ttcctcgctt cgctcctaac cgacaactgg ttcctgttcc cattgatcac
     2701 tctctccggg ttggctggtt cgctcttcga ctccttcctc ggcggaactg tgcaagccat
     2761 gtactactgc cccaccgata aaaaagagac cgagaaacac cccctgcaca cctgcggcac
     2821 acccaccgtc cacacacgcg gatggcaatg gctggataat gaccgtgtca acttcgcctg
     2881 tggagcggca ggtactttgg ttgctctgtt gcttttcttt atttaaccca catgaacaaa
     2941 cctgggcaac tgcgttccta ttccttcatc ctcgccactc tgcgatacag gaaactgatc
     3001 gcattcctca cgcgagtcat gatcgcattg cgcagaaaaa aggtcatcct catctttggc
     3061 atgagtcgca gcggaaccag catgctggga aaattcctct cgcttggcaa gtcctccatc
     3121 tacatgcacg aacccgagac cgaattgatg aaacagcact tcggccccga ccgtcactac
     3181 acccagcaaa acttctggga ctttgtccac tctgaagtgc cgaccgagct caaggtccac
     3241 acactggcct gcatttcgct ttttgccgtg ctcaaggcgc cgatcttttc gaaggccatc
     3301 gtcataaaac ccatcagttt tgtggaagtc atcggtgaat ccagcgaagc gctgaccaac
     3361 gccaacatcg tctacatttg ccgccacccc gcaggcagaa gtgaaagcat cctgcgccag
     3421 ctccagcatg accagggcat tgaagaaatt tctctcgaag ccctcgaaca gatcagccag
     3481 gattggggcc gcaccaacaa gacgatcaag gaactgcacc gtcaaaaccc caagtggctg
     3541 tgggtttatt ttgaaaaact gacgggcgat cccgtagccg aattcaagaa actgtacacc
     3601 caactcggcc tgccatggaa ccagtccatt gctgatgaga tcgagaaaag caccacaggc
     3661 atggacggcg gttactacaa catccaacgt gacagccgca agcaggccga taaatggcgc
     3721 aactctctca ccacagagca gatcgaatcc gttcgcaaag ggaccctgcc cttcgaaac
//
LOCUS       DAOQLC010000249         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_12954_length_3687_cov_13.246145, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000249 DAOQLC010000000
VERSION     DAOQLC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12954_length_3687_cov_13.246145"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PL000_21205"
     CDS             <1..474
                     /locus_tag="PL000_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91457.1"
                     /translation="HFTGFGLYDQKVIDILKGLQDPYPYFRGLIADIGFEPAIIEFTQ
                     PARKFGKSKSNLFYLYEEAMLGLTSYTKLPLRLATIIGFSSALISFLIGMFYLVYKLL
                     FWSSFSVGTAPITIGLFFFASIQLIFLGIIGEYISMIYIHVLNRPLVYEKERINF"
     gene            498..1508
                     /locus_tag="PL000_21210"
     CDS             498..1508
                     /locus_tag="PL000_21210"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNC91458.1"
                     /translation="MPEDLVDEKPKRTSRLMLLVSVILAGVFLYFALRNLDWQVFFAA
                     LKGARYSYLPIILFLININYLFRAMRWRGLLLNEKHISVSTVFWANMTGYLGNNVLPA
                     RAGEVMRAAYIARKENISISFIIATGVTERLVDLAALVIVGAVSLFFVDALPRSVQDA
                     LKSFAIIALAGVVFLFLLPYFHGIFSRILAMLSFLGDSLKTKIQEFTSRFIEGIKVIS
                     RAERGLPFIFFTALIWLLDGIGTVFFALAFNESLTLMQAFLFIAALGLSSAIPSTPGY
                     VGVYQFVAVTVLAPFGIKRESALAMILVSQALNLLIVSLWGGLGLWFGSQGMVKSTPQ
                     GN"
     gene            1511..3625
                     /locus_tag="PL000_21215"
     CDS             1511..3625
                     /locus_tag="PL000_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91459.1"
                     /translation="MDSNFFLQDIVGISLAFLLFSFVFIFPGYVIGWGFNLLKHRSQP
                     LMLQLGTGIFISISVIPAFLFLVYRFLPAKIGIGILFAFAIAAVYIYFTQNKASDNNA
                     VPASEKGYQKSVLGLILVWFLFSVFVSADLQIGKRLYFSNNSYDMLTRVAVVDAMTRT
                     GVPPVNPSYYPGEEVRLNFLYYYWYILASVVDMLGGPLVSALHAMIASISWCGIALFA
                     TVSTYMQVRAPSENAWKKTFIGIQLFAVSGLDCIIIILAAVREGYLPVNGGIEGWNMP
                     IMSWLNAITWVPHHVAAAIACIFALLLLLHEKNERLPAKLLVSVMIGLAFASAFGLSV
                     WVMFVFAFFWAVWAIISLLEKKYASLGVMVLSGFFALIFISPFLAGLVSPTGETSTAS
                     SASPVAFYIRPFMASVVFPEGTPSIVLGTANFFMLPLNYLFELGFFFLMAAIWIQEFY
                     SRNKNTNPIYKAELVLAAVTTVVLSFLYSTLISINDLGIRGWLPLQIILIVWAVDVIT
                     MFMGNEKWITPRIFRVFQNPKSLGLVLGVTLTLGLLTTSLEAISLRTWAILIDMNVTD
                     FPNSISVDNHLGERTYYARLAYSYISENTPNNIIIQSNPQNQMERPSGLYATRQMVIS
                     DRTIYGVPIPVYAKLSQDIGSIFRRKTSSWDDIDTKCTQYKIDILVFKDTDRVWESLP
                     DLKTIRPPLYENPYYALYSCGK"
BASE COUNT          852 a          913 c          803 g         1119 t
ORIGIN      
        1 cactttacag gatttggcct gtatgaccaa aaagtaattg atattcttaa aggtttgcaa
       61 gatccatatc cttacttccg tggcttgatt gctgatatcg ggtttgagcc cgctatcatt
      121 gaattcacac aacccgcgcg gaaattcggt aaaagtaaaa gcaatctttt ttatctttac
      181 gaagaagcca tgctgggcct taccagttac accaaacttc ccctaaggct cgccaccatc
      241 atcggatttt ccagtgcctt gatcagtttc cttatcggca tgttctacct ggtctacaaa
      301 ctactctttt ggagcagttt ttcagtcggt actgcaccaa tcaccattgg gcttttcttt
      361 ttcgcctcaa tccagcttat ctttctaggt atcattggtg aatatatctc catgatctat
      421 attcacgtgc tgaatcggcc gctggtctac gaaaaggaac ggatcaactt ttgaatcaac
      481 agagcactcc tgtacccttg cctgaagatc tagtggatga aaagccgaaa cgcaccagcc
      541 gcctcatgct tctggtctcg gtcattctgg caggggtatt tttatatttc gccctgcgta
      601 atctcgactg gcaggtattt tttgcagccc tgaaaggcgc caggtactcc taccttccga
      661 tcatcctctt cttgatcaac atcaattacc tttttcgtgc catgcgttgg cgtggactat
      721 tattgaacga aaagcacatt tcagtttcca ctgttttttg ggcaaacatg actggatatc
      781 tcggcaacaa tgtattgccc gcacgcgccg gggaagtgat gcgtgccgca tacatcgcgc
      841 gcaaggaaaa tatttccatc agttttatca ttgcaacagg tgtcactgaa aggctggtgg
      901 atctggctgc cctggtcatt gtcggcgctg tctccctttt ttttgtagac gcacttccca
      961 ggtccgtgca ggatgcgttg aagagttttg caatcatcgc gcttgcaggc gtcgttttcc
     1021 tgttcctcct gccttatttc cacggaatat tcagccgcat ccttgctatg ctttcatttc
     1081 tgggcgattc actcaaaaca aagatacagg aatttacgtc acgattcatt gaagggataa
     1141 aagtgatttc gcgggcagaa cgtggtctgc catttatctt cttcaccgcc ctgatctggc
     1201 tgctggatgg aatcggtaca gtcttttttg cactggcatt taatgaatct ttgaccctga
     1261 tgcaggcctt tttattcatt gccgcgcttg gactatcaag tgcgatccca tccacaccag
     1321 ggtatgtggg ggtttatcaa tttgtagcgg tgacggtgct tgcgccgttc ggcatcaagc
     1381 gtgaatctgc ccttgccatg atccttgttt cgcaggcgct gaatctattg atcgtgtctc
     1441 tgtggggcgg actcggcctt tggtttggat cacaaggcat ggtcaagtca actcctcaag
     1501 ggaattaaac atggactcga atttcttcct tcaagacata gtcggtatct cgcttgcatt
     1561 tttattattt tcttttgtct ttatatttcc cgggtatgtc atcggctggg gattcaacct
     1621 gttgaagcat agaagccaac cgctcatgtt gcaacttggt acagggattt ttatctcaat
     1681 ctcggttatt cctgcctttc tatttctggt ctatagattc ctacctgcaa agatcggcat
     1741 tggtatcctt tttgcatttg ctattgctgc tgtatatatt tactttaccc aaaacaaagc
     1801 cagcgacaac aatgcggtgc ctgcatcaga aaaaggatat caaaaatccg ttcttggtct
     1861 gattttggta tggtttctgt tttcggtatt tgtttctgcg gatcttcaaa ttggaaaacg
     1921 cctctacttc agcaacaact cttacgatat gctgacacgg gtggccgtgg tggatgcaat
     1981 gacccgtacc ggcgttccgc ctgtaaatcc gtcttattac cctggcgaag aagtgcgtct
     2041 gaatttcctc tattactact ggtatattct cgcgagcgtt gtggacatgc tcggcggccc
     2101 gctggtcagc gccctgcatg ccatgattgc aagcatcagt tggtgtggaa ttgcactctt
     2161 tgcaactgtg tcaacctata tgcaagtccg tgcgccttct gaaaacgcat ggaaaaaaac
     2221 ctttatcggg atacagttgt tcgcagtcag cggattggac tgcatcatca tcatccttgc
     2281 ggcggtaagg gaagggtatc tgcctgtcaa cggcggcatc gaaggctgga atatgccgat
     2341 tatgtcatgg ctcaatgcaa taacctgggt gccgcatcat gttgcagccg caattgcatg
     2401 tatctttgca ttattacttc tcctgcatga aaagaacgaa cgcttacccg ccaagcttct
     2461 tgtttcagtc atgatcgggt tggcttttgc ctccgctttc gggttatcgg tctgggtaat
     2521 gtttgtattt gcgttcttct gggcagtttg ggcgatcatt tcacttttgg aaaagaaata
     2581 tgccagtttg ggagtaatgg tattaagtgg tttttttgcc ttgatcttta tctcaccttt
     2641 tctggctggg cttgtgtcgc caactggtga aacctcaaca gccagcagcg cgtcaccagt
     2701 ggctttttac atccgtccct tcatggcaag cgtcgttttc cccgaaggca ctccctccat
     2761 tgttttggga accgccaact ttttcatgct tccgttgaac tatctgttcg agctgggctt
     2821 tttctttctc atggctgcta tctggattca agagttttac agcaggaata aaaacacaaa
     2881 ccccatctac aaagcggagc tggtccttgc ggctgtaacg acggtagtgc tttccttctt
     2941 atactccacc ctaatctcga tcaacgattt aggcatccgt ggttggctgc ctctccaaat
     3001 cattcttatt gtctgggctg ttgatgtaat taccatgttc atgggaaatg aaaaatggat
     3061 tacacccagg atattccgtg tgttccaaaa cccaaaaagc cttggtctgg ttctgggggt
     3121 tacactgacc ctcggcctgc tcaccacaag tctcgaagcc atttcacttc gcacctgggc
     3181 catactcatt gatatgaacg tgaccgactt ccccaactcc atcagtgtgg ataatcatct
     3241 cggcgaaaga acttattacg cccgtctcgc ttactcttac attagcgaaa acactccaaa
     3301 caatatcatc atccagagta atccacaaaa tcaaatggag cgccccagcg ggctgtatgc
     3361 cacccgccag atggtcattt ctgatcgtac gatctacggg gtgccaattc ctgtttacgc
     3421 aaaactctca caagatattg gcagtatttt cagaagaaaa acctcttcat gggatgacat
     3481 tgatacaaaa tgtactcaat acaagataga catacttgtc ttcaaagaca cagacagagt
     3541 ctgggaaagc ctgcctgatc tgaagaccat ccgcccgccc ttgtacgaaa acccgtatta
     3601 cgccttgtac tcgtgtggaa aataaaccta acttttcgta aaccgcatcc gccactcaaa
     3661 cagtttattc aatgcctcaa ttggcga
//
LOCUS       DAOQLC010000250         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13028_length_3676_cov_21.841204, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000250 DAOQLC010000000
VERSION     DAOQLC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13028_length_3676_cov_21.841204"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PL000_21220"
     CDS             <1..553
                     /locus_tag="PL000_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91460.1"
                     /translation="SGVRLVVSDAHEGLKQAIAKVIGATWQRCRVHFMRNALAHVPRK
                     QHSMVAAVIRTAFVQENQQEARQQWRETADKLRERFAKVAELMDSAEDDVLAFMSFPK
                     EHWPQLASTNSLERLNKEIKRRSRVVGIFPNNAAIMRLVGTLLAEQTDEWQVTRRYMS
                     QETLARVLSKEDQANLLPQSEAA"
     gene            719..3112
                     /locus_tag="PL000_21225"
     CDS             719..3112
                     /locus_tag="PL000_21225"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNC91461.1"
                     /translation="MNAITNVVGQSLEDGARVARLKKAVDEATYGGCIERAVLWSEYY
                     MDEANDGKSTAVQIAEATRHVLANKTVKIYPDELIVGNFTAKRVAGICYPELGGLGIM
                     IGVDKLPTRKVNPLEISEDEQAKLQSYIPFWMDKNMVYLAFDGQEEQMRFVKEQIEAL
                     QYQLYEAGGIAHLAPGYEKVIKLGAEGIIAEVEAFEAQTNDPSKLDFYQSVKISMNAL
                     AEFGDRYATEARRLAEAEADPQRKLDLERIAEVCARVPRYGATSFYEAVQSMTLTHIA
                     IFQETIGETTCPGRVDQFLNSYYEEDLAAGRISRQEAKDILGAFCVKLCETIPMYSDE
                     LTSMLGGLTSWEVVTIGGQDSEGNDATNELSFILLELADELRMRQPNFHVRIHENTPK
                     AFYDEVIRVNFGPGSAPAMYNDETIIESMMNVGYSLEDARDYVAIGCVEPTAPGKTLG
                     STDAGMYNMALALEMALNEGCQFGSDRQIGVKTPPVSSMKSMDDVLDAYKAQVKYQLG
                     KLHADLQKCEQFHARYHPTPLTSATLEGCLEQGVCSTQGGAKYNFSGIQGTGMAVVAD
                     SLGAIESAVFEDKWLTLEELVAHLKDNLSDEVVRTRLKGIDKFGNDIPKADAWMKWVG
                     DHYSDSIHALGKNTRGGQYLAGVYSNTSHTHFGSLTSATPNGRRAGETFASGFAPENG
                     ADKRGTTALLNSMNRIDYKKFANGINFNIKLDASSYDCEEGRDALASLYKVYFKRHGM
                     QVQANMLDPKILIEARDNPELHPNLLIRVSGYSAYFNDLSPEMQDEVISRSSNGAEQ"
     gene            3112..>3676
                     /locus_tag="PL000_21230"
     CDS             3112..>3676
                     /locus_tag="PL000_21230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNC91462.1"
                     /translation="MGPDASLIDAIAVTEPRKASLYFDIKRNTLDDGPGIRSVVFFKG
                     CPLSCTWCQNPESINTGYELSYDAEKCISCHDCADACPEQAIDLSSPDKIIRDKCTQC
                     FKCVDVCPSGALERCGNDIDMDHLVEKVLEDEAFFEASGGGVTVSGGEALMQMDSVGE
                     LFSRLKQRNVHTLIQTAGLFNYKVFENL"
BASE COUNT          874 a          970 c         1077 g          755 t
ORIGIN      
        1 gtcaggcgtg cgactggtgg tctcggatgc ccacgagggc ctgaagcagg cgattgccaa
       61 ggtcatagga gctacctggc aacgctgccg ggtgcacttc atgcgcaatg cgctggccca
      121 tgtcccgcgc aagcagcact ccatggtggc cgccgtgatc cgcacggcct tcgtgcagga
      181 gaaccagcag gaggcacgcc agcagtggcg agaaaccgct gacaagctgc gagagcgctt
      241 cgccaaggta gcggagctca tggacagcgc cgaggacgac gtgcttgcct tcatgagctt
      301 cccgaaagag cactggcctc agctggcatc gacgaattcc ctggagcgat tgaacaagga
      361 aatcaaacgc cgctcgcggg tggtgggtat cttcccgaac aacgctgcca tcatgcggct
      421 cgtgggaacg ctactcgctg agcaaacaga tgaatggcag gtgacacgcc gctacatgag
      481 ccaagagacg ttggcgcgcg tgctgagcaa ggaagaccag gccaacctac tgccacagtc
      541 tgaggccgcc tgaagcaatg aaactggaga ccggaattac accacttgac gggacactac
      601 cactccttgt actctccata gtatgtgctt cgtttaagac ggggcgacat gtaaagcaac
      661 cgtagcttca aaaacaatat taaacttttg agtcaagcgc tgagacgcga ggtgaaatat
      721 gaatgcaatt actaatgtcg ttggtcagtc actggaagat ggtgctcgag tcgctcgcct
      781 gaagaaagct gtcgacgagg ctacctacgg tggctgcatc gagcgcgcag tgctttggtc
      841 agagtattac atggatgagg ccaacgatgg gaaatctacg gccgttcaga tagctgaagc
      901 gacacgccac gtcctggcca acaaaacggt gaaaatctat cctgacgagc tgattgttgg
      961 caacttcacc gccaaacggg tcgccggcat ctgttatccg gagttgggcg gtctgggcat
     1021 aatgattggt gtcgacaaac tacccacccg caaggtcaat cctctcgaga tcagcgaaga
     1081 cgagcaggca aaactccagt catatattcc gttctggatg gacaagaata tggtttatct
     1141 tgcctttgac gggcaggaag agcagatgcg ctttgttaag gagcagattg aagcactgca
     1201 gtaccaattg tatgaggctg gtggtattgc tcacctggca cccggctacg agaaagtgat
     1261 caaactgggt gccgagggca tcatcgccga ggtggaggcc ttcgaggcac aaaccaacga
     1321 tccctccaag ctggacttct accagtctgt gaaaatctca atgaatgcgc tggcagagtt
     1381 tggtgaccgc tatgcgaccg aggcgcgtag gctggcagag gctgaagcgg accctcagcg
     1441 gaaacttgat ctagagcgga ttgcggaggt ctgcgctcgc gtaccacgtt atggcgcaac
     1501 cagtttctat gaagcggtcc aatcaatgac cctcacccac atcgccattt tccaggagac
     1561 aataggtgaa accacctgtc cgggccgcgt ggaccaattc ctgaattcct actatgaaga
     1621 ggatttggcg gctggacgta tttcaagaca agaggcgaaa gatattctgg gagctttctg
     1681 cgtaaagcta tgcgaaacca tccccatgta ctccgacgag ctgaccagca tgctcggtgg
     1741 attgaccagc tgggaggtag tgaccatcgg tggtcaggac tccgagggca acgatgcgac
     1801 caatgagctg agtttcattt tgttggagct cgccgacgag ttacgcatgc gccaacccaa
     1861 cttccacgtc cgtattcacg agaacacacc caaggcattc tacgatgagg tcatccgggt
     1921 gaacttcggc ccgggttcgg cgcccgccat gtataacgac gagaccatca tcgagtccat
     1981 gatgaatgtc ggttattccc tggaggatgc cagggactat gtcgccatcg gctgtgttga
     2041 gcccacggcg ccgggcaaga ccctgggttc caccgacgcc gggatgtaca acatggccct
     2101 tgccctggaa atggcgctca acgagggatg ccagtttggc tctgaccgac agatcggtgt
     2161 caagaccccc ccggtgtcca gcatgaaaag catggatgac gtattggatg cctacaaggc
     2221 gcaggtgaag tatcagctgg gcaagctgca cgcggatttg cagaagtgtg agcagttcca
     2281 cgccaggtat catccaacac cccttaccag cgctacactc gaaggctgtc tcgagcaggg
     2341 tgtttgctct acacagggtg gcgcgaagta caatttctcc ggtatccagg gcaccggcat
     2401 ggcggtcgtg gccgattccc tgggggccat tgagagtgct gtgttcgagg acaagtggct
     2461 cacccttgag gagctggtcg ctcacctgaa agacaatctc tcggatgagg ttgtgcggac
     2521 caggctgaaa ggcatcgata aatttggcaa cgacattccc aaggcggatg cctggatgaa
     2581 gtgggtcggt gatcactact ccgattccat tcacgcgctg ggcaagaata cccgtggcgg
     2641 ccagtacctg gcgggcgtgt actccaatac ttcccatacg cattttggca gtttgactag
     2701 cgctacaccc aacggtcgcc gggctggtga gaccttcgcc tcgggttttg cgcctgaaaa
     2761 cggagcggac aagcgaggta ccactgcgtt gttgaattcc atgaaccgca tcgactacaa
     2821 gaagttcgcc aatggtatta atttcaatat caagctggat gcctccagtt acgactgtga
     2881 agagggacga gatgcgctag ccagcttgta taaggtgtac ttcaagcggc acggtatgca
     2941 ggtgcaggcc aatatgttgg atccgaaaat cctgattgaa gcacgggaca acccggagtt
     3001 gcaccccaac ctgctgattc gggtatcggg ctactcggca tacttcaacg atctatcacc
     3061 tgaaatgcag gatgaggtga tatcccggag cagtaatggg gcggagcagt aatggggcct
     3121 gatgcaagcc tgattgatgc gatagcagtt accgagccaa ggaaggcttc gctgtacttc
     3181 gacatcaaga gaaacaccct ggacgacggg ccgggtattc gcagcgtggt gttcttcaag
     3241 ggctgcccgc tgtcttgtac ctggtgccag aatccggaaa gcatcaacac cggttacgaa
     3301 ctctcctatg atgcagagaa gtgcatcagc tgtcacgact gtgctgacgc ctgccccgag
     3361 caggcgatcg acctgagttc gcccgacaaa atcatccgcg acaaatgcac ccagtgcttc
     3421 aagtgcgttg atgtctgccc atccggtgct ctggagcgct gtggcaatga catcgacatg
     3481 gatcatctgg tggagaaggt gcttgaagac gaggccttct tcgaggcctc cggcggcggg
     3541 gttactgtct ccggcggcga ggcgctgatg cagatggatt cggtcggcga actgttctcg
     3601 cggctgaagc agcggaatgt tcacacgctg atccagacag cgggcctatt caattacaag
     3661 gtatttgaaa atctgg
//
LOCUS       DAOQLC010000251         3676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13029_length_3676_cov_20.090859, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000251 DAOQLC010000000
VERSION     DAOQLC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13029_length_3676_cov_20.090859"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PL000_21235"
     CDS             <1..272
                     /locus_tag="PL000_21235"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91463.1"
                     /translation="ELGTGNLQPGSHLSVGFGLTAFNDLAPRYRDAAWSDVSLGAYYA
                     EPVVEAYSWGIVDGTSATTFSPDQAMTRGEFAKTLYRILMLYGTF"
     gene            543..1958
                     /locus_tag="PL000_21240"
     CDS             543..1958
                     /locus_tag="PL000_21240"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91464.1"
                     /translation="MSKKVFTVLLLAVFVLAQFSIASAAQVTADGTLTGPDFMPAGAG
                     STVTLEWTATGLPAGTYVDIYARLMGSGNTYDVLECNNVAFINGSSNGSGTFDYVFTG
                     GGVYGNPADGEYWEFLIAFDDAASCTTLPANANVDANFSASSYVDGTELEGILVIPHP
                     DFTASGVVACNTFEYWALASDTYGLANPSDPYSGFGSWNAKTTGTFAPAPIGAEDELL
                     SWKITLPATASGLWQFSIDPEDAAGNLNAGPYYFRNSNVSADELEDCADFSDVAGHEH
                     EVYIRYLADLGLISGFTDGTFGPDNTLTRAEASALIEISNGYDATTLPTSAPAGCDFT
                     DVAATDWFAGWVWQACDDGFMNGVGGGMFDPNNLLTRGQIVTIFNNILELGAGNLQPG
                     SHLSVGFGLTAFNDLAPRYRDAAWSDVSLGAYYAEPVVEAYSWGIVDGTSATTFSPDQ
                     AMTRGEFAKTLYRILMYFGAF"
     gene            2172..3590
                     /locus_tag="PL000_21245"
     CDS             2172..3590
                     /locus_tag="PL000_21245"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91465.1"
                     /translation="MSKKVFTVLLLAVFVLAQFSVASAAAVAGADITLTNTSEFLSEA
                     LAAPWASTTFEWTLGALPAGTTYYVDIYARNAGVNTYASSGECLNVATVNGGVAASGS
                     FTYDFTSTTADGDTWEFVMTVDDTASCADGANVETANVVMHSAYVDGHSINGFGAVPP
                     SDPAIVSDWVACNIFEYWAVGSDAYGLPASTPYSGIGDWNPQITGTFAPPAPIGPADE
                     VLEWSITLPATASGAWTITISPEDAAGNRDWVAPPYYYRNGGLIVPSELEDCADFSDV
                     SGHEYELYIRYLADIGMISGFADGTFGPDATLTRAEAATLIEISNGYDATTLPTSAPA
                     GCDFTDVAASDWFAGWVWQACADGYMNGVGGGLFDPNNLLTRGQIVTILNNVNVAGPT
                     GGILNYGGNVMDINWGNLYREAAWTDVSIGAFYAQPVINAYGIGIADGTSATTFSPDQ
                     PITRGEFSKMLYRALSIWTGTF"
BASE COUNT          715 a         1049 c          954 g          958 t
ORIGIN      
        1 tggaattggg tacgggcaat cttcagccgg gctctcacct gtctgtcggc tttggtctaa
       61 ctgccttcaa tgatctggcc cctcgttacc gtgacgcggc ctggagcgat gtgtccctcg
      121 gtgcgtacta tgccgaacct gtggtggaag cctacagttg gggcattgtg gatggcacct
      181 ccgccaccac cttctccccg gatcaggcca tgacccgtgg tgaattcgcc aagaccctct
      241 acagaatttt gatgctttac ggtactttct agttttctgt aaaaatgtag attcaaaccc
      301 tggagcggac ctgtcagcgg gtccgctcct tttttgttct ccaatcgtga tacggcaaga
      361 tttatcttgc aacttgcctg atttgcaaca aagtgaattt gtggcggtag ttccaattgc
      421 gtcgtcctat gcacagtgat tgacggaatc gcgattctct gtaaaatagg cgcattcagg
      481 tcgccatcat ctgaatctag taatggatgg cagaaatcaa atcttataca aggagaaaag
      541 caatgagcaa gaaagtattt accgtattgc tgctggctgt gttcgtgttg gcacagttca
      601 gcatcgcctc cgctgcacaa gtcaccgcgg atggcaccct caccggcccg gactttatgc
      661 ccgcaggtgc aggctccact gttaccctgg aatggaccgc cactggtctg cccgctggca
      721 cctatgtgga tatctatgcc cgtctcatgg ggtcaggcaa cacctatgat gtgcttgaat
      781 gtaacaacgt agccttcatc aatggttcca gcaacggtag cggcacattt gattatgtgt
      841 tcactggtgg tggtgtgtat ggcaaccctg ccgatggcga atattgggag ttcctgatcg
      901 cgtttgacga tgcggcttcc tgcaccaccc tgcctgccaa tgccaatgtg gatgcgaact
      961 tctctgcctc ctcctatgtg gatggcactg aattggaagg tattctggtc attccccacc
     1021 ccgatttcac tgcttctggc gttgtggcct gcaatacctt tgaatactgg gcgctggctt
     1081 ccgacactta tggtttggcg aacccctctg atccgtactc tggatttggt tcatggaatg
     1141 ccaagaccac cggcaccttt gctcctgctc caattggcgc agaagatgag ctgctctcct
     1201 ggaagattac cctgcctgcc accgcaagcg gcttgtggca gttctcgatt gaccccgaag
     1261 atgcagctgg taatctgaat gctggccctt actacttccg caacagcaac gtttcggctg
     1321 acgaacttga agattgcgcg gatttcagcg atgtggctgg tcacgaacat gaagtgtata
     1381 tccgctacct ggccgacctc ggcctgatca gcggcttcac cgatggcacc ttcggcccgg
     1441 acaacaccct gactcgcgcc gaagcctctg ccctgatcga gatctccaac ggctacgatg
     1501 ccaccaccct gccgacctct gctcctgcgg gttgtgactt caccgacgtg gccgccaccg
     1561 actggttcgc tggctgggtc tggcaggctt gtgatgacgg cttcatgaac ggtgtgggcg
     1621 gcggtatgtt cgacccgaac aacctgctca cccgcggaca gatcgtcacc atcttcaaca
     1681 acatccttga acttggtgcg ggcaatctcc agccgggttc tcacctgtct gtcggttttg
     1741 gtctgactgc cttcaatgat ctggcccctc gttaccgtga cgcggcctgg agcgatgtgt
     1801 ccctcggtgc gtactatgcc gaacctgtgg tggaagccta cagttggggc attgtggatg
     1861 gcacctccgc caccaccttc tccccggatc aggccatgac ccgtggtgaa ttcgccaaga
     1921 ccctctacag aattctgatg tatttcggtg ccttctaatt ttcccgcaat agagtaaatc
     1981 gaaactctgg agcggacctg tcaccgggtc cgctcctttt ttgtttgacc gatggtcatg
     2041 ccctgaccgg cgtcctattt acagcggttg acgagatttt gtttccctgt aaaattggcg
     2101 ccattcaggt agccatcgcc tgaatctagt actggatggc agagatcaac ttttcaaagg
     2161 agaatgtatt aatgagcaag aaagtattta ccgtattgct gctggctgtg ttcgtgttgg
     2221 cgcagttcag cgtcgcctcc gctgccgctg tggcgggtgc cgatatcacc ctgaccaata
     2281 cctcggaatt cctgtccgaa gctttggcag ctccgtgggc ttccactacc ttcgaatgga
     2341 cgctgggcgc acttcctgct ggtactactt actacgtgga tatttatgcc cgcaatgctg
     2401 gtgtgaacac ttatgccagc agtggtgaat gtttgaacgt ggcgaccgtg aatggcggtg
     2461 tggctgcctc cggtagtttc acttacgatt tcaccagtac aaccgccgat ggtgacacct
     2521 gggaattcgt aatgaccgtt gatgataccg cttcctgtgc tgatggtgcg aatgtggaaa
     2581 ccgccaatgt ggtcatgcat tccgcctatg tggacggtca cagcatcaat ggctttggtg
     2641 ctgttccccc cagtgacccc gccattgtaa gtgactgggt tgcctgcaat atctttgagt
     2701 attgggccgt tggttctgat gcttatggtc ttcccgcttc aaccccatac tctggtattg
     2761 gcgattggaa tcctcagatc accggcacct tcgcccctcc tgctcccatt ggcccggccg
     2821 atgaggtgct tgaatggtcc atcaccctgc ccgccactgc cagtggcgcc tggaccatta
     2881 ccatcagccc tgaagatgcg gctggcaata gagactgggt agcccccccc tactactacc
     2941 gcaatggcgg tctgattgtg ccttccgagc ttgaagactg tgctgatttc agcgatgtat
     3001 ctggtcatga atatgaactc tatattcgct atctggccga catcggcatg atcagcggtt
     3061 ttgctgatgg aactttcggt cccgatgcca ccctgacccg cgccgaagca gccactctca
     3121 tcgagatctc caacggttac gatgcgacca ccctacccac ttcggccccc gcaggctgtg
     3181 acttcaccga cgtggccgcc tctgactggt tcgctggttg ggtctggcag gcttgtgctg
     3241 acggttacat gaacggcgtt ggcggcggtc tcttcgaccc gaacaacctg ctcacccgtg
     3301 gtcagatcgt caccatcctc aacaacgtga atgttgccgg tcctacaggt ggtatcttga
     3361 actatggcgg caatgtcatg gatatcaatt ggggaaatct gtaccgtgaa gctgcctgga
     3421 cggatgtttc cattggtgca ttctacgcac agcctgtgat caatgcctac ggtattggca
     3481 ttgcggatgg cacttctgcc accaccttct cgcctgatca gcccatcacc cgcggcgaat
     3541 tctccaagat gctctaccgt gccctgagca tttggactgg tactttctaa acaatcatag
     3601 taacgaaccc tggagcggac ctgtacaggt ccgctccttt tttgttcgat caacgtcgtc
     3661 ctcgcacaga ttgacg
//
LOCUS       DAOQLC010000252         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13108_length_3659_cov_22.398169, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000252 DAOQLC010000000
VERSION     DAOQLC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13108_length_3659_cov_22.398169"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..742)
                     /locus_tag="PL000_21250"
     CDS             complement(47..742)
                     /locus_tag="PL000_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91466.1"
                     /translation="MNSSSSPDKITLLIADDHPSTRMGVRASISRFSDLEIIGEASNG
                     IDAQYMVSIDPPRVILLDLNMPDLPPVVFTKWVREFHPETAILVLTFHIDNISYLAGL
                     MDAGISGYLSKAISMDDLANSIRRVANGEVIFDNQQRMAAHQWKKEVQEKIRLLTHQE
                     MEVIKLLMAGKNNQTIAEALNISPKTVTYHLTSLFSKLQVQSRLEATLWAMDHLLDNL
                     DLFPYLNRDKNLG"
     gene            894..1439
                     /locus_tag="PL000_21255"
     CDS             894..1439
                     /locus_tag="PL000_21255"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HNC91467.1"
                     /translation="MTSYCEYCNTHPEDTFNKKYHDTQYGFPLKDDDELFERLVLEIN
                     QAGLSWITILKKAENFHKAYNGFKIEKIAKYTEKDRARLLADAGIIRNRLKVNAAIVN
                     AQKVLELKKEFGSFKNWLDQNHPLTKDEWTKLFKKTFIFTGGEIVNEFLMSTGYLPGA
                     HQPDCPVYKKVASLRPAWMKK"
     gene            1436..2548
                     /locus_tag="PL000_21260"
     CDS             1436..2548
                     /locus_tag="PL000_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91468.1"
                     /translation="MTAVPRANRDVTLDILKGIGCLFMIVAHSSLNFGGYERFKFWYG
                     LAPVLFFSAAGVTAALQAQKYKPRGVLLTYAFLLLLGYSFNRITDPGFLKEVEFDIIQ
                     MIAVGSSVIYLVEHYARPPAWIYFLLGLLAFAMKFLLQALFSGFAVVGLTNLIFPPGI
                     FPIFPWLFLFFFGLFAYRNHNVVNLAAGLGFGLLLLVLQQTNFPLDIENKWDMSVGYF
                     LVSCILLFGTFYLLRLIPFPPQPRGLGLLTFLGQNSLLFLYVHFPIILYFKEIHIQNI
                     YVVIFENPYLFWVLTLALTILVMVILLLLPRSKALTALFSQLPVWLVLVFLVFAVGIY
                     IRNRFFTYYIEIALGLLIALFYPRLANLFKQSASSS"
     gene            2635..3645
                     /locus_tag="PL000_21265"
     CDS             2635..3645
                     /locus_tag="PL000_21265"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNC91469.1"
                     /translation="MTPTFVIAGRLNREYVLPPSGQPLLDSPGGNLLYAAGGLAVWDS
                     SIGLVGRVGEDYPRQWMRALNERGFDIRGIRTLRELQNVDLRSFIAFTDTNERSHSNA
                     VSHFARRQLTFPKSLLGYQPPDESRKDPREPEPASPSALDVPKDFRDVRYVHICPFDF
                     VSQSQMVNLFKGGSNQTVSLDPAPGYMVPAFWRDLRLVLQGVTIFQPSEDELRSLFWG
                     ETNDLWEMARRISEYGPQIVVIKRGSQGQMLYDVPGNHRYELPAYASRMVDPTGAGDA
                     FCGGFLAGFQKTNDPLLSALHGSVSASLKIEGSGPFYELDVMPGLAEARLFALKEMVR
                     EV"
BASE COUNT          798 a          949 c          891 g         1021 t
ORIGIN      
        1 ttctaatatc cccccgcaat tttgacactg gataaatatc tagtctttaa cctaggtttt
       61 tatcccggtt caggtatggg aacaggtcca ggttgtctaa caaatggtcc atagcccaaa
      121 gcgtggcttc aagcctcgat tggacctgta acttggaaaa caggcttgtc agatgataag
      181 ttaccgtttt gggagatata ttcagggctt cggcaatggt ctggttgttt ttgccagcca
      241 ttaataattt gatgacttcc atctcctggt gggttaggag acggatcttt tcttgaactt
      301 cctttttcca ttgatgcgcg gccatccttt gttggttatc gaaaataact tccccgttgg
      361 caacacgccg aatgctgttg gccagatcat ccattgatat tgctttgctt aggtatcctg
      421 aaatacctgc atccatcaaa cccgcaagat aagagatatt atcaatgtgg aaagttaata
      481 ccagaatggc ggtctcagga tgaaactcgc gcacccattt cgtgaatacg acggggggga
      541 ggtcgggcat gtttaggtca aggaggatca ctcttggagg atcgattgac accatatatt
      601 gagcgtcaat gccgttgctt gcttcaccaa tgatttccag atccgagaat cgcgaaatgg
      661 atgcccgaac ccccattcgc gttgatggat gatcatctgc aatcagtagg gtgatcttat
      721 ccggtgaaga agagctattc atatggtcca atatctcctt ggttgagtga caggtacagt
      781 gccccagtga cctgcccggc aattttatca ttgttgggta ctgggcaatg ttcgcaagga
      841 caaaccaggt gtttggagga ttacttttca cttaggcata gtaaaattac tttatgacct
      901 cctactgcga atactgtaac acccaccccg aagatacctt caataaaaaa tatcacgata
      961 cccaatacgg ctttccgctc aaggatgacg atgaactctt tgaacgcctt gtattggaga
     1021 tcaaccaggc tggcctttcg tggatcacca ttttgaaaaa agcggaaaac ttccacaaag
     1081 cctataacgg attcaagatc gagaagatcg cgaaatacac cgagaaggac cgtgcccgtc
     1141 tgctggctga tgcgggtatc atccgtaacc gcttgaaggt caatgcggcg atcgttaacg
     1201 cgcagaaggt ccttgagttg aaaaaggaat tcggctcgtt caagaactgg ctcgatcaaa
     1261 accatccgct caccaaagac gagtggacca aattatttaa gaagaccttt atttttacag
     1321 gcggtgagat cgtcaacgag ttcctcatgt ccacggggta tttgcccgga gcgcatcaac
     1381 ccgattgtcc cgtgtataaa aaagtggcga gcctgcgccc ggcgtggatg aaaaaatgac
     1441 cgccgttccg cgtgcaaacc gcgatgtcac attggacatt ttgaaaggca tcggctgtct
     1501 gttcatgatc gtggcgcatt ccagcctgaa cttcggcggc tatgaacgct tcaaattctg
     1561 gtacggactg gcccccgtac tttttttttc cgcggcaggt gtcactgccg cattgcaggc
     1621 ccaaaagtac aaaccgcgcg gcgtgttgtt gacctatgcc tttttgcttc tgcttgggta
     1681 cagcttcaac cgcattaccg accctggttt tctcaaggaa gttgaattcg acatcattca
     1741 gatgatcgcc gtggggtcaa gtgttattta tctggtggag cactacgccc gcccgcctgc
     1801 atggatatat tttcttttgg ggctgttggc ttttgcaatg aaattcctgc tgcaggcttt
     1861 gttttctggc tttgcggtcg ttggcctcac caatctcatt tttccgcctg gcatctttcc
     1921 gatcttcccg tggttgtttt tgttcttctt cggtctattt gcctaccgca atcacaacgt
     1981 cgtcaacctc gcggcgggcc ttgggtttgg tctgcttctt ttggttttac aacaaacgaa
     2041 ctttccgctc gatatcgaga acaaatggga tatgtcggtt gggtattttc tggtcagttg
     2101 tattttgctt ttcggcacct tttacctgtt gcgcctgatt cccttcccac cccagccgcg
     2161 cggacttggc ctgttgacct tcctcgggca gaattcactt ttgttcttgt acgtccattt
     2221 tccgatcatt ttgtatttca aggaaattca catccagaat atttacgtgg tcatttttga
     2281 aaatccctac ctcttctggg tgctgaccct tgccctgacc atcctggtga tggtcatcct
     2341 gctccttttg ccgagatcga aagcgctgac cgcattattc agtcaattgc cagtttggct
     2401 cgtgctggtc tttctggtct ttgccgtggg catttatatt cgcaatcgtt tttttaccta
     2461 ctatattgag atcgcactgg gcttgttgat cgcattgttc tatccgcgtt tggcgaacct
     2521 tttcaagcaa tccgcctcat cctcttgatc cacttgcaca atgggcgcag aagttgaaaa
     2581 cactttgcgc agccacgaaa cagaatatcc cattttctat gtatacttgc ccccatgacg
     2641 ccaacttttg tcatcgcagg aagactaaat cgtgaatatg ttctgcctcc ctcaggtcaa
     2701 cctttgctcg attcgcccgg tggaaacctg ctttatgccg caggcggact cgccgtatgg
     2761 gattccagca ttggcctcgt tgggcgggtg ggggaggatt atccgcggca atggatgcgc
     2821 gccctcaacg agcgcggttt tgatatccgc ggcatccgca ctctgcgcga attgcaaaac
     2881 gttgacctgc gttccttcat cgccttcacc gacaccaacg aacgcagtca ctcgaatgcc
     2941 gtttcgcatt ttgcccgccg ccagttgacc ttccccaaat cgctgcttgg ttatcagcct
     3001 cccgacgaat cgcgcaaaga cccgcgtgag cctgagcccg cttctccttc tgcgcttgat
     3061 gtgccgaaag attttcggga tgtccgctac gtgcatatct gcccctttga ctttgtcagc
     3121 cagagccaga tggtcaacct cttcaaaggc ggatccaacc aaaccgtgag cctggatcct
     3181 gctcccggct atatggttcc tgccttttgg cgtgacctga ggctggtgct gcagggcgtc
     3241 accatcttcc aaccctctga agatgaattg cgctcgctct tttggggtga gaccaatgac
     3301 ctgtgggaga tggcccgccg catcagcgag tacggaccgc agatcgtcgt catcaaacgc
     3361 ggctcacagg ggcaaatgct gtacgatgta ccaggcaacc accgttatga gcttccagcc
     3421 tacgcttcac gcatggtgga ccccaccggc gcaggcgatg ccttctgcgg cggattcctt
     3481 gctggcttcc aaaagacgaa cgatcccctg ctttcggctt tgcatggcag tgtctcggct
     3541 tcgctcaaaa tagaaggcag cggccccttt tacgagttgg atgtgatgcc cggtcttgcc
     3601 gaagcgcgct tgtttgcgtt gaaggaaatg gtgcgggaag tgtgaaaggg aaaggatga
//
LOCUS       DAOQLC010000253         3658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13115_length_3658_cov_13.997502, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000253 DAOQLC010000000
VERSION     DAOQLC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13115_length_3658_cov_13.997502"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..3538
                     /locus_tag="PL000_21270"
     CDS             506..3538
                     /locus_tag="PL000_21270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91470.1"
                     /translation="MEAIEQKSKPVRNVVISVHGIRTFGQWQERLARLVRQADPQAIS
                     YSYHYGFFSVLAFLVPPLRGIEVARFRRHLQRIVDMHPQDRISVVGHSFGTHLIGWAL
                     AKSFKTHGTPIHQIILAGSVLRSTFDWHDLIEKGVANRIINDCGIDDNVLLLSQVAVL
                     FTGMAGRLGFTGAVGAQLCNRYFLGGHSHYFLGDNGQADDAFMLRYWLPLLVQSSEPE
                     EVDQRLNGGPLQGVYVWMIQNADVVKVGVLLSIGFLVWQMLYATPRAMALAESLSHAR
                     AVALQQIGVDEQTPHGVANLLNIIRRDPEDVTSRELVSRWLPELENYSEVVSQRGLPA
                     LMVWNGHNLLVSKDSTVDLGGAVILTYGFSRDGTQLIAFDADGTIRIVQVYDAKNLLS
                     ISTRKEVRESIVLKAPVETSSAEEYESIGEVLGKANPQAARLSFDGELESISFRESPD
                     GIYLWGVGTSAAESAGLSEGVLFAVNRKTMSYAFCRTSNDLRLLLSGERVRAFATYQP
                     DDPDTEQSQKDTARAEVMMELRWPDRTNGFSSVKSDGKESTPQLPTALVEQVKRLGKE
                     DLPSQRIGSMGLNQVLAKRGAIESDEWNDESPVLVYPNLRPAHTSWTVSMLSQNNMSS
                     IGVDLLAEIPHARIVTNEESKAVHRQLRAYIQKMGEEHLPAEYLSAYLSDGYDEGDIV
                     LVPERKHVVYLLRGSDGAKYGVTVYCVAKDISGAVEFCDYIRARGNFVSVAISDDGRY
                     LVGGDFAGLGQPGIHFRNLASNRMLEVNEQPSGGVRFLTFAPGETTVFASTPYGILEY
                     AMESDAVRYVRRYELPALAAELAEGTADYSRRDIRASATHLFYVSYGGVVQALSRKTG
                     LADWLVRLPVSGAGLAFDSSTQILAVYGDRCVHLMHSSSGARIARPLCVTDVAASDQK
                     VETEESITLVRFEAAGLLNVFTDARKFSRKFLPPKEAAIEVTEKMSFYSRWLARIGLS
                     RSAEMEAQQQDWIRRTGYDHLHNPVRALTVLPGQDRTK"
BASE COUNT          872 a          844 c         1051 g          891 t
ORIGIN      
        1 cagcaagccc acgctgccga cgaaggaaag ctaatagggg gcattagctt tacatgaagg
       61 ggacggcggc aattctgccg ccgttatgcc agaatggtgg ttatgtgacc ttcaggttgc
      121 gggcgcacga tccgcctgcg catctgagcg aggcgagcac gtgccggaca agatgcagcg
      181 ctcggagcaa agcaaaacca gatcaccgtg ctaaagcacc agaagttgac gtacgactag
      241 aaatgagaaa gaactggttg gcttcatctg agtgcgcatg ttgtccagtc gtttgcagga
      301 ctcggtgatt gaaggagaaa gagcatgaaa atggccattg tggtgcgact ctgaactgct
      361 tttagcgcgc cgtgcgctct gcgggcttga ggccattctt ggtatttgcc cctcgcttca
      421 gaaatacaag caagagagac aaagcaatca caatatacta aataaaaatt tagtttcaac
      481 gatacattta actccaaaag cataaatgga agcgattgag caaaaatcta agcccgtgcg
      541 taacgttgtt atcagtgttc acggcattcg cacgttcggt cagtggcagg agcgattggc
      601 aaggctcgtg cggcaggctg atccgcaggc catttcttat agttatcact acggcttttt
      661 ctcggtacta gcgtttttgg ttccaccatt gcgcgggatc gaggttgcgc ggtttcggcg
      721 gcatttgcag cgcattgtag atatgcatcc acaagatcgg atatcggtcg tcggccacag
      781 cttcggcacg cacttgatcg ggtgggcgct agccaagagt ttcaagacgc atggtacacc
      841 gatacaccag atcatcctgg cggggagtgt attgcggtcg acgttcgact ggcatgatct
      901 aatcgaaaag ggtgtggcta accggatcat caatgattgt gggattgacg acaatgtact
      961 gctgctgagc caggtggccg ttttgttcac cggtatggcg ggtcggttgg gcttcactgg
     1021 cgcggttggc gcacaactat gcaatcgcta tttcttgggt ggtcatagtc actatttctt
     1081 gggcgacaat ggtcaggcgg atgatgcttt catgcttcgc tattggctgc cattgttggt
     1141 tcaatcgagt gagccggagg aggtagatca gcgtctcaat ggtggtccgt tgcagggggt
     1201 gtatgtatgg atgatccaga atgcagatgt agtaaaagtg ggagtcctgt tgtcgatagg
     1261 ctttctagtc tggcagatgt tgtatgccac accccgtgcc atggctctag ccgagtccct
     1321 ctctcatgcg cgtgccgtag cgctgcagca gatcggtgta gacgagcaaa cgccacacgg
     1381 tgtcgccaat ctgctgaaca ttattcgaag agacccggag gatgttacat cccgcgagtt
     1441 ggttagccgt tggttgcctg aattggagaa ttattctgaa gttgtaagcc agcgcggtct
     1501 gccggcgctg atggtgtgga acggacataa cctacttgtt tcgaaggatt cgaccgtaga
     1561 tctcggcggg gctgtgattc tgacttacgg tttcagtcga gatggcactc agctaattgc
     1621 atttgatgca gatggcacga ttcgtatagt tcaggtgtat gatgccaaaa atttactttc
     1681 aatctcgact agaaaagaag tccgcgaatc tatcgtattg aaagccccag tcgaaacgtc
     1741 gagcgcagaa gaatatgaat caattggcga ggtattgggc aaggcgaatc cgcaagctgc
     1801 tcggttgtct ttcgacggtg aactcgaaag tattagtttt cgggaaagtc cagatgggat
     1861 ttatctctgg ggggttggta ctagcgctgc cgaatcggcg ggtctgagtg agggtgtgtt
     1921 gttcgcagtt aaccgcaaaa ctatgtcata tgctttttgt agaacatcaa atgatctgcg
     1981 ccttctccta agcggtgagc gcgtacgcgc attcgcgact taccaaccgg atgatccaga
     2041 cacggagcag agccaaaaag acactgcgcg ggccgaggtc atgatggagt tgcgctggcc
     2101 tgacagaacc aacggcttca gctccgtcaa gtcagacggt aaggagtcta caccgcaact
     2161 tccaacggca ctagtcgaac aggttaagcg tttgggcaag gaagacttgc ctagccagag
     2221 gataggcagt atggggctta accaagtgct tgctaaacgc ggggcaattg aatcggatga
     2281 gtggaacgat gaaagccctg tgttggttta tccaaacttg cggccagcgc atacatcgtg
     2341 gaccgtaagc atgctgtctc aaaataatat gtcctcgata ggcgtagact tgctagcaga
     2401 aattcctcac gctcggatcg ttacaaatga agagtcgaag gccgtgcata gacagttgcg
     2461 tgcatatatt cagaaaatgg gtgaagagca tctccctgca gagtatctgt ccgcatacct
     2521 cagcgatgga tatgacgaag gagatattgt gcttgtaccc gagcgcaagc atgtcgtcta
     2581 tctgctgaga gggtcggatg gggcgaagta tggcgtcacc gtttattgtg tagctaagga
     2641 catatcggga gctgtggagt tctgtgacta tattcgagca agaggaaact tcgtctccgt
     2701 cgccattagt gacgacggtc gttacttggt cggtggcgat tttgcaggtt tgggccagcc
     2761 tggcattcat tttcgcaacc ttgcttccaa ccgcatgcta gaggtcaatg agcagccctc
     2821 aggtggcgtc agattcctca cgttcgcgcc aggcgagact accgtctttg ctagcacacc
     2881 ctatggcatt ttggaatatg ctatggagag tgacgctgtg cgctatgtgc gccgttacga
     2941 actgccagcc ttggccgctg aactggccga gggcaccgct gactacagcc gaagggatat
     3001 tcgcgcatct gctacgcacc tcttctatgt ctcatacgga ggtgtggtgc aagcgctgtc
     3061 acgcaagacc ggtttggctg attggctggt ccgactgccg gttagtggtg cagggttggc
     3121 cttcgattcc agtacacaga tacttgcggt ttatggagat cgatgcgtgc atctgatgca
     3181 ctcatcatca ggtgcacgca tcgcccgacc cttgtgcgtt acagatgttg ctgcgagtga
     3241 tcaaaaggtc gaaacggaag agagtattac cttggtgcgc ttcgaagccg ccggattgct
     3301 caacgtcttc actgatgcaa ggaaattcag ccggaaattt ctaccaccaa aggaagcggc
     3361 cattgaggtg actgaaaaga tgtctttcta tagtcggtgg ctagcaagga tagggctttc
     3421 taggagtgct gaaatggaag cacagcagca ggattggatc aggcgaactg ggtatgacca
     3481 tctgcacaat ccggtacggg cgttgacggt attgcctggt caagacagaa caaaatgaaa
     3541 aaagcgctcc atttatacag attgaacttc aacctttccc gacctacacc ttcttcaaac
     3601 ggtcccaaca gagcaatcct ttcagtttgg tagctcgggt gagcgcagcg taacccgc
//
LOCUS       DAOQLC010000254         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13208_length_3645_cov_19.410306, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000254 DAOQLC010000000
VERSION     DAOQLC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13208_length_3645_cov_19.410306"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PL000_21275"
     CDS             complement(<1..226)
                     /locus_tag="PL000_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNC91471.1"
                     /translation="MAVSETTNTKESERSVLLPQRHPEHDLFICDVLDAIPKDDLGSM
                     EHPVFSLATRPDTRILKYEHRNVKIQITPSV"
     gene            1052..1258
                     /gene="kleE"
                     /locus_tag="PL000_21280"
     CDS             1052..1258
                     /gene="kleE"
                     /locus_tag="PL000_21280"
                     /inference="COORDINATES: protein motif:HMM:NF036636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KleE stable inheritance protein"
                     /protein_id="HNC91472.1"
                     /translation="MTNIIQFPMMGNSPPAPDPPSPSPQPPHLDGARGKAIISLVKGV
                     WVVTVLVVSHREFDSPGVAKVFEE"
     gene            1279..2400
                     /locus_tag="PL000_21285"
                     /pseudo
     CDS             1279..2400
                     /locus_tag="PL000_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611739.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2623..2928
                     /locus_tag="PL000_21290"
     CDS             2623..2928
                     /locus_tag="PL000_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator KorA"
                     /protein_id="HNC91473.1"
                     /translation="MKKRLTDAEFQEAIKGLDVGSQTLEIARGVLIDGKPQKEFAASL
                     GLSKGAISQAVNRVWAAHEEKSLPHGYVRVSAVLPEYRAFIVKKWAEDATKQRQFKK"
     gene            2925..>3645
                     /locus_tag="PL000_21295"
     CDS             2925..>3645
                     /locus_tag="PL000_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011114061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC91474.1"
                     /translation="MRTLVVANQKGGVGKTSTLVHLAFDFLERGLKVVVIDLDTQANA
                     SYTLQSFRSGLIASGLFADVPSDSWPQTAIDFTDPDSPRMMLIASDIGLANLEKISLS
                     EAGAKFRDAIKAIAEQGFDVCLIDTAPSLGVSMAAALLAADYVLSPIELEAYSIQGIK
                     QIVTTIANLRKANPKLKFLGMVPSKVDARNPRHGRHLQELERAYPQLMIPTGVGLRSS
                     IADALASRVPVWKIKKTAARKA"
BASE COUNT          845 a         1038 c          986 g          776 t
ORIGIN      
        1 tgacgctcgg cgtaatttgt atcttcacat tgcgatgttc gtacttcagg atgcgcgtgt
       61 ccggccgagt tgccagggaa aaaaccggat gctccatgct tcccaagtcg tccttgggga
      121 tcgcgtcgag cacgtcgcaa ataaacaagt cgtgttccgg atgccgctgt ggaagcaaca
      181 ccgatcgttc tgattccttt gtattcgtgg tttcgctgac ggccatgtgc tcttcgtgct
      241 atcgctgacg tttccagcat ttcgtggttt cgatgacggc aatgataaat tcgtggtttc
      301 gttgacgcaa gctctttttc gtggtttcga tgacgcctta acgtgtttct gctgacgctt
      361 tttcgtgttt tcgctgacga gacttccgaa attcatttgt tttttcagaa cgttacagca
      421 ctatttcggc cgttaacact acttaactct atatttaaca aacaccacgc cacccaactc
      481 acctgtggat aacttgcaaa cccacgtcgc acgccatagc acgaagggaa ttgagcactc
      541 ccctgggcaa ccacccaaaa aggcacaacg tgcatccgaa actacctccc cttgaagaaa
      601 tccaccccaa aagagataaa aagcggttta acgccaggaa caagcaccca cttggccgcc
      661 aaaaatatca gtgtttagct aacgtatgtt gacgcacagg gacttttgta cttatataga
      721 catgaacgct agcgcttccg aaagacgatt taccgaagaa cctcttccta atgaacaatg
      781 cacaaggcat ccatttgctt tatgagcttc cgggtcttgc tgctaccagc gaggcgtgcc
      841 catatggacg cagcagcgaa ctcgcgcgaa gaaaaatgct taccgcccaa ggcgtatttc
      901 accgtcgagc agaccaagaa accagcggac cggcgagggg aaatgctccg acatgaggtc
      961 ttggtgtgtt taccccccag caccgtcaca tcaaggtccg ggcccggact tccagcacca
     1021 ttgcacatgc catagcgtgg agggtcaagt aatgacgaac atcatccagt ttcccatgat
     1081 gggcaattct cctcctgctc ccgatccacc atcgccgagc ccccagccac cccaccttga
     1141 tggcgctcgg ggaaaagcca tcatttcctt ggtcaaaggc gtttgggttg tcaccgtcct
     1201 agtagtcagt catcgtgaat ttgatagccc gggcgtggcc aaagtctttg aggagtgatg
     1261 aactttcagg agacgtagat ggcagcgatc aaatatcgag tgaccctgac ggcggaagaa
     1321 actaaagaac tggaatcctt ggtgcacaag ggaaagagcg cggcccggaa gcaaacgcgg
     1381 gccaggattc tgctgaaggc ggcagaggga tgtcaggacc aagagatcat ggatgcgctg
     1441 gatgtttctt ccagcacggt cggcaggatt cggcaaaggt gtgtcgagga agggatcgag
     1501 gcagctctgg aggaccgacc acggccggga gcagcgcgca agctgaccga aaagcagtgc
     1561 gcgcaggtga ttgcggtagc gtgtacccct gcgcccgagg ggtatgatca ttggaccttg
     1621 cgactattgg cggatcaagt agtacaactg ggcttttcgg actccttcag ccatgaagca
     1681 gtccggcagc ttttaaaaaa acactttgaa gccgtggcag cagcaggaat ggtgcattcc
     1741 cgcggtcggc gcagattttg tcgcgcccat ggaagacgtg ctggatctgt acgcagcgcc
     1801 ttacgatccg gcttatcctg tcgtgtgctt cgatgaaagc cccaagcagt tggtcgcgga
     1861 agtgcgtacg cccattgcgg tcgaacctgg cgctccggca cgctatgaca cggaatatga
     1921 gcgcaatggc gttcgcgacg tgatgatgat ctgcgaaccg aagcgaggtt ggcgagaggt
     1981 gttgctcacc gagcgtcgaa ccaagatcga tttcgcccac atgatgaagc acatcgtcga
     2041 gtgttatccg gaagcgacga ccattcgctt ggtgatcgac aacctgaaca cgcacaaacc
     2101 ggcttcgctc tatgaagcct ctttccgccc gaagaggcgc gggctatcgc caggaagctc
     2161 gaattccact acaccccgaa acatggcagt tggctcaaca tcgccgaaat tgagttggcc
     2221 gtcctctcca ccatgtgcct gtcgcaacgt attcccgacg aagccactct acggcgccat
     2281 atcgatgcca acatccgcga gcgcaatgcc aaggccatcc cggtcaagtg gcaattcacc
     2341 acacaagatg cccgacgcaa gctcgctcgt ctctatcctc gcgtttcagt tccaagttga
     2401 ctgactacta ggcatgtcgg cttttcacgc accaagcgaa ccggctgcat ccgccaatca
     2461 ccgttcacac tcactaccga gccttgcgag acagaatgta aatttttagc cgctaaaggg
     2521 attgcttgct catcacgttt ggcccaacta ctattgatgc ttggcggcag tcatcgcctt
     2581 ttttcccaat gggcgcggac tacgaaatgg gtggtgcaat ccatgaagaa acggctaacc
     2641 gatgccgaat tccaagaggc catcaagggt ctggacgttg ggtcgcagac cttggagatc
     2701 gcgcgcggcg tgctgatcga tggcaaacca caaaaggagt ttgccgcgtc gctgggtttg
     2761 tccaagggag cgatttcgca ggcggttaat cgcgtgtggg ctgcccacga agagaagagt
     2821 ctgccacacg ggtacgtccg ggtgtcggca gtcctgccgg agtatcgggc attcatcgtc
     2881 aagaaatggg ctgaggacgc cacgaaacag cggcaattca agaaatgaga actctggttg
     2941 tcgcaaacca gaagggcggg gttggtaaga cttcgactct cgttcacctc gcgtttgact
     3001 tcctcgagcg aggtctaaag gtggtcgtca tcgacctcga cacccaggcc aacgcctctt
     3061 acacgctgca gtccttccga tccggcctta tcgcaagcgg actgtttgcc gacgtaccgt
     3121 ccgactcgtg gccgcagacc gcaatcgact tcaccgatcc cgattcgccg cgcatgatgc
     3181 tgatcgcgtc agatatcgga ctggcaaacc tggagaagat atccttgtcc gaagctggcg
     3241 cgaagtttcg ggacgccatc aaggctatag ccgagcaggg cttcgacgta tgcctgatcg
     3301 acaccgctcc ttcccttggc gtcagtatgg ccgccgctct gctggcggcc gactatgtcc
     3361 tgtcgccgat cgagcttgag gcgtacagca tccagggcat caagcagata gtcacgacca
     3421 ttgccaacct tcgcaaggca aaccccaagc tgaagttcct tggcatggtg cccagcaagg
     3481 tcgatgcccg caacccgcgt cacggtcgcc acctgcaaga actggaacga gcctaccccc
     3541 aactgatgat tccgaccggg gtcggccttc gcagcagcat tgccgacgca ctggcgtccc
     3601 gcgtgccggt gtggaagatc aagaagaccg ccgcccgcaa ggcca
//
LOCUS       DAOQLC010000255         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13616_length_3580_cov_17.808794, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000255 DAOQLC010000000
VERSION     DAOQLC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13616_length_3580_cov_17.808794"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..723)
                     /locus_tag="PL000_21300"
     CDS             complement(1..723)
                     /locus_tag="PL000_21300"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HNC91475.1"
                     /translation="MKLITTYALAAVALLVFYTGNVLAAGEAFEGRKKCSSCHKSQAK
                     SWGDTAHAKAMESLKPEVKKEAKLKAKLDPAKDYTQDKDCVGCHVDGYGKQGGYTVET
                     PNKYLTGVGCESCHGPGKKYRGDHRKAGQTFEKSGKTTQRKVLAEKGQDFAFEESCNA
                     CHMNYEGSPWKGAKPPYTPYTPEVDAKYKFEFDKMVKNEKAMHEHYKLDGAFEGEPKF
                     KYHDEFQASAKVAKKDDKKGKE"
     gene            complement(818..1846)
                     /gene="haoB"
                     /locus_tag="PL000_21305"
     CDS             complement(818..1846)
                     /gene="haoB"
                     /locus_tag="PL000_21305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine oxidation protein HaoB"
                     /protein_id="HNC91476.1"
                     /translation="MVASTESQRPDDGRWSDKLLPLLGITLVTGGLILLGWFAYLWFT
                     PGEAPYQYQLIKEGEAKQFPELELEHWPDLTISKYEIRTTGIDKPLAQAYFGRRGQGY
                     PVMLNWSNHTAEALIGLDRKPSELTTLAEVIGKHATPESVILAWWDTSRQIQLLTGRN
                     TLFKTPLYEPLIVPSLWQENQKAILNYEQQMVTEKASETEREQFKQFSEALLLPPEEG
                     VKRLRALVKPEQEALLVIHVTDLYKIGLMYPEKFGVAYKNFPMTGNIHGMINHMKVQL
                     KEHGYTTYTPQSITDNDIRVFFLTDEKSTQTLLAHMLPFTGRDPPTELKVAQLVYQQG
                     GYWVYKLP"
     gene            complement(1850..3400)
                     /locus_tag="PL000_21310"
     CDS             complement(1850..3400)
                     /locus_tag="PL000_21310"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNC91477.1"
                     /translation="MKRYKDPAQGAGPGTMAKYWEPIPYGIYLDPATFYKPPVSMKET
                     AGRKECVECHTDESPVWVQAWKRSTHANLDKVRNMKADDPLYYKKEKLEEVEGNLRSM
                     GKLGADEKLKEVGCIDCHVEVNAKKKADHTKDLIMPTADVCGTCHLREFAERESERDT
                     MIWEDGQWPDGRPSHALDYKANVETTVWAAMPQREVAEGCSMCHTNQNKCDSCHTRHE
                     FSAAESRKPEACATCHSGVDHNNWEAYSMSKHGKMASMFGDKWNWEVPLKDAYAKGGQ
                     NAPTCAGCHMEYEGQYAHNMVRKIRWANYPFVPGIAENINSEWSEARLDSWVVTCTQC
                     HSERFARSYLELMDKGTLAGLAKYQEANDVVHQLYKEGLLTGQKTNRPAPPAPEKEGY
                     AYFAQLFWSKGNSPAAIELKVLEMHENDLAKMHVGLAHVNPGGWTYTEGWGPINRAYV
                     EIQDENTRIREMIALQARVNKLEAGKTSLLDLNGTSEKISLGGLGGGMLLAGTIALIG
                     WRNKRKQS"
BASE COUNT          723 a          905 c          795 g         1157 t
ORIGIN      
        1 ttactctttt ccttttttat cgtctttttt agctacctta gcgctagctt ggaattcatc
       61 atgatattta aacttaggct cgccttcaaa tgcaccatcc aatttgtaat gctcgtgcat
      121 cgccttctca ttttttacca tcttgtcaaa ttcaaattta tatttcgcat caacttccgg
      181 tgtgtatggg gtgtaaggcg gcttcgcacc tttccatggc gatccttcat agttcatgtg
      241 gcatgcgtta cagctttctt caaatgcaaa gtcttgccct ttttctgcca gtactttacg
      301 ttgcgtggtt ttacccgatt tctcaaaggt ttgccccgct ttacgatgat ctccacggta
      361 tttcttaccc ggtccatggc aagattcaca tccaacccca gttaggtatt tgttcggtgt
      421 ttcaactgta tagccacctt gcttgccata accatcaaca tggcaaccta cgcagtcttt
      481 atcttgcgta tagtctttag cagggtctag cttcgctttg agtttggctt cttttttgac
      541 ttccggtttt aacgattcca tcgctttcgc atgcgccgtg tctccccacg atttcgcttg
      601 cgatttatgg catgagctac atttcttgcg tccttcaaat gcttctcctg ccgctaatac
      661 attccctgta taaaacacta gcagcgccac agcagctagt gcataagtgg ttattaactt
      721 catgtcctct cctctcttca acttaataga taaaaatagt gttgttcctt accctaattg
      781 tacgcgatca tcagattgtg taaatgggtc tattcgatta cggtagttta taaacccagt
      841 aaccaccttg ttgataaacc agttgcgcta ctttgagttc cgtcggggga tctctgcctg
      901 taaacggaag catatgggcc agtagcgttt gggtactttt ctcatcggtt aggaaaaata
      961 cgcgaatatc gttatcggtg atcgattgcg gcgtataggt ggtatagccg tgttccttga
     1021 gctgcacttt catgtggttg atcatgccgt ggatgttccc tgtcatcgga aagttcttat
     1081 acgcaacgcc aaacttttca gggtacatga gcccaatttt ataaagatcg gttacgtgta
     1141 tgactaagag ggcttcttgt tccggcttaa ctaaagcgcg tagccgtttg accccttctt
     1201 ccggtggtag gagcagtgct tcgctaaatt gcttgaattg ttcacgttcc gtttcgctgg
     1261 ccttttccgt taccatctgt tgttcatagt tcaaaatggc tttttggttt tcttgccaga
     1321 gtgagggaac aatgagtggt tcgtaaaggg gggtcttaaa cagggtgttt cgtcccgtta
     1381 gcagttgaat ctgacgggag gtgtcccacc aggcaagaat gacgctctcg ggtgtggcgt
     1441 gcttaccgat aacttcagct aaggtggtca gttccgatgg tttacggtct agtccaatca
     1501 acgcttcagc ggtgtgatta ctccaattga gcatcacggg gtacccttgg ccgcgtcgtc
     1561 caaaataagc ttgggctagc ggtttgtcga ttccagtcgt tctgatttca tatttgctaa
     1621 tcgtcaggtc tggccagtgt tctaattcca gttccggaaa ttgtttagct tcgccttctt
     1681 tgatgagttg gtattggtaa ggcgcttccc cgggtgtaaa ccataggtaa gcaaaccagc
     1741 ccagcaaaat gagacctccc gttacaagag tgattccaag taacgggagt aatttgtctg
     1801 accagcgccc gtcatccggg cgctgacttt ctgttgatgc aaccacgctc tagctttgtt
     1861 tacgcttgtt acgccatccg atcagtgcaa tcgttccagc aagtaacatg cctccaccta
     1921 agccacccag tgagatcttt tcactggtgc cgtttaggtc aagtaggctg gtttttcccg
     1981 cttcgagctt gttaacgcgc gcttgcagtg caatcatttc acgaatgcgt gtgttttcat
     2041 cttgtatttc aacatacgca cggttgattg gaccccatcc ttcggtatag gtccatccac
     2101 ctgggttaac gtgtgctaat ccaacgtgca tcttggctag gtcattttcg tgcatttcga
     2161 gtactttcaa ctcgatcgct gctgggctgt tacctttcga ccagaagagt tgcgcaaagt
     2221 aagcatagcc ttctttttct ggtgcaggtg gcgcgggacg attggttttt tgtcctgtta
     2281 acagtccttc tttgtagagt tggtgtacaa cgtcgttagc ttcttggtat ttagccaggc
     2341 ctgctaaggt tcctttgtcc atcagttcta agtaagagcg tgcaaaccgt tctgagtgac
     2401 attgggtaca ggtaactacc catgagtcta agcgtgcttc tgaccattcg ctgttgatgt
     2461 tttctgcaat tcctggaaca aacggatagt tcgcccaacg gatcttacgt accatgttgt
     2521 gggcgtattg gccttcatat tccatatggc agcctgcgca ggtcggtgca ttttgtccac
     2581 ctttggcata cgcgtctttt aatggcactt cccagttcca tttgtcgcca aacattgaag
     2641 ccattttgcc atgttttgac atggagtagg cttcccagtt gttgtggtct acaccactat
     2701 gacaggtcgc acaggcttct ggtttacgag attccgccgc agaaaattca tggcgtgtgt
     2761 ggcaggagtc acatttgttt tggttggtat ggcacattga gcagccttcc gcaacttcac
     2821 gttgtggcat cgcagcccat acggtggttt ctacgtttgc tttgtagtcc agtgcgtgtg
     2881 atggacgccc gtctggccat tgaccatctt cccatatcat ggtgtcacgt tccgattcgc
     2941 gttccgcaaa ttcacgtaag tgacaggttc cacatacgtc agctgtcggc atgatcaggt
     3001 ctttggtgtg gtccgctttc ttcttcgcgt tcacttcaac gtggcagtcg atacagccca
     3061 cttctttcaa tttctcgtct gcacccagtt tacccatcga gcgcaggtta ccttctactt
     3121 cttctaactt ttctttcttg tagtacaacg gatcgtccgc tttcatgttg cgtactttat
     3181 ccaagttagc atgggtgctg cgtttccatg cttgtaccca taccggcgat tcgtctgtgt
     3241 ggcattctac gcattcttta cgaccagccg tctctttcat cgataccggt ggtttgtaaa
     3301 aggtcgcagg atccaaataa attccatacg gaatcggttc ccagtatttc gccatcgttc
     3361 caggcccagc cccttgtgcc ggatccttgt aacgcttcac taacgcatca tacgtctcct
     3421 tcggcgtagc cttcgctctg tccaaattca acgccttgta tgtctcgtcc ggtaccgtcg
     3481 aaatgttcgc ttgcaatacc ccagcaaaca tcaccccagc taccagcatc attcccctct
     3541 tccatcccag gctcttcatc tattctctcc tccttttctt
//
LOCUS       DAOQLC010000256         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13666_length_3571_cov_25.949374, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000256 DAOQLC010000000
VERSION     DAOQLC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13666_length_3571_cov_25.949374"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="lysX"
                     /locus_tag="PL000_21315"
     CDS             complement(<1..844)
                     /gene="lysX"
                     /locus_tag="PL000_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HNC91478.1"
                     /translation="MQRRLKIGVLYSRVRVEEKWIFGAMEKRGIDYDRLDDRAIHFDL
                     EKPEAWQQYDAVLERSISYNSGLYALRLLNSFGVPTVNTAAVAEVCGDKLMTSAVLAR
                     DGVPQPHNVTAFSSEAALDAIEAFGYPVVLKPVVGSWGRLLAKINDRDAAEAVLEHKS
                     TLGSVQHSVFYIQEFIEKPGRDLRIIMIGDKVLTGMYRNSEHWITNTARGGSGELCPI
                     TPEIEDICLRASKAVGGGLLGVDLVEHPEKGLVVNEINHTMEFHTMQPVSGLDIAGEI
                     VEYVV"
     gene            complement(889..1059)
                     /gene="lysW"
                     /locus_tag="PL000_21320"
     CDS             complement(889..1059)
                     /gene="lysW"
                     /locus_tag="PL000_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HNC91479.1"
                     /translation="MSTVVCPECEAQVELAAGTEAGEIIVCSDCGVDLEVTSLDPATV
                     ELAPMEQEDWGE"
     gene            complement(1063..2424)
                     /gene="argH"
                     /locus_tag="PL000_21325"
     CDS             complement(1063..2424)
                     /gene="argH"
                     /locus_tag="PL000_21325"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC91480.1"
                     /translation="MTLWGGRFSQKLDDLAWALNASLPVDQHMAFQDVEGSIAWAAAI
                     HRAGILTDEEHASISTGLDTVRNEFASGEFIFQPSDEDIHTAVERRLSELIGPAAGKL
                     HTGRSRNDQVATDFRLWMLNKIPELHSAILILQNALVEQAELAGETIMPGYTHLQRAQ
                     PILLGHWWLSHYWALKRDVERLQDLTKRVSVLPLGSGALAGAPFALDRDALAESLGFD
                     EVSQNSLDAVSDRDFAAEYLFVCSMASVHLSKLAEQIVLYTTAEFGFFELSDAFSTGS
                     SLMPQKKNPDVFELTRGKAGTMIGLLTGLMSTLKGLPSTYDKDLQEDKAPVFQATDTL
                     LSILPVMAAALRTITVKSERMRGAIDSTMMATDLADYLVEKGVPFREAHSLAGKVVRA
                     AGEAKVRMEELPLEAYQAISPVFEADVYQVFDPLESINKRNTVGGTSLQSVKNQIKKI
                     KGE"
     gene            complement(2486..>3571)
                     /locus_tag="PL000_21330"
     CDS             complement(2486..>3571)
                     /locus_tag="PL000_21330"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC91481.1"
                     /translation="ASEFVIHDMKEEFVSEYLFPMLKAGAVYEHKYLLGTSVARPLIA
                     KHLVEVAHQTGADAIAHGATGKGNDQVRFELTVMALDPRLKIIAPWREWEIRSREDAL
                     NYAAKHNVPVTATIKSIYSRDSNLWHLSHEGGLLEDPWNEPEEVMFQMSTSPENAPEQ
                     GEYVEIEFESGNPVSVNGEKMSPAKIVSTLNAIGGNHGIGRVDLVENRLVGMKSHGVY
                     ETPGGTILLYAHRELESLILDRETLHYKQVIAVKYAELTYNGLWFTPLREALDAFVNS
                     TQGPVTGTVRLKLYKGNIISAGRKSKFSLYREDFATFGQEDVYDQSHAEGFIRLYGLS
                     MKVRALNGLPVSGLDMPKPDYSKFKRD"
BASE COUNT          816 a         1021 c          894 g          840 t
ORIGIN      
        1 tcaccacata ctccacgatc tcaccagcaa tatccaatcc gctgaccggc tgcatggtgt
       61 gaaactccat cgtgtgattg atctcattta ccacgagtcc cttctcaggg tgctccacca
      121 gatccacacc gagcagacca ccgcccaccg cttttgaagc ccgcaggcaa atatcttcga
      181 tctcaggtgt gatcgggcat agctcaccgc tgccgccgcg tgccgtgttc gtgatccaat
      241 gctccgagtt gcggtacatg ccagtcagca ctttgtcacc gatcatgatg atgcgcaggt
      301 cacgacccgg cttctcaata aattcctgaa tataaaagac cgaatgctga actgaaccca
      361 gagtcgattt atgttccagc acagcttccg ccgcatcgcg gtcattgatc tttgccagta
      421 accgtcccca cgaaccgaca accggcttca acaccacggg gtagccaaat gcctcgatcg
      481 catccagcgc cgcctcggat gaaaatgccg tcacattatg aggctgcggc acgccatccc
      541 gtgcaagcac ggcactggtc atcaatttgt caccgcatac ctcagccact gccgccgtgt
      601 tgaccgtcgg cacaccgaac gaattcaaca aacgcaaggc gtacaagccg ctgttgtaac
      661 tgatactgcg ctccaacacc gcatcatatt gctgccatgc ttcaggtttt tccagatcaa
      721 agtgaatggc acggtcatca aggcggtcat aatcaatgcc gcgtttttcc atcgcgccga
      781 agatccattt ctcttcaacg cgcacacggg aataaaggac gccaattttc aatcgtcgtt
      841 gcataatatt ctccgtaggg gcacgataca tcgtgcccct acaataatct attcacccca
      901 atcttcctgc tccatcggcg caagctcaac cgttgcaggg tccaacgacg ttacctcgag
      961 gtccactccg caatccgagc agacgatgat ctcaccggct tcggttccgg cagccaattc
     1021 aacctgggct tcacattcgg ggcatacaac tgtagacatg atttattctc ctttgatctt
     1081 cttgatttgg tttttgacag actgaaggct cgtgcctcca accgtatttc ttttattgat
     1141 cgattccagc ggatcaaaga cctgataaac atccgcttca aacactggac taattgcctg
     1201 atacgcttcg aggggcagtt cctccatccg aaccttcgcc tcccccgccg cgcggacgac
     1261 tttccctgcc agcgagtgcg cttctctaaa tggcacgcct ttctccacga gataatccgc
     1321 caaatccgtc gccatcatcg tggaatcaat cgcgccacgc attcgttccg acttgaccgt
     1381 gatcgtccga agcgccgccg ccatgacagg caggatcgag agcaaggtgt ccgttgcctg
     1441 aaagacgggt gctttgtctt cctgtaaatc cttgtcgtat gtagatggca accccttgag
     1501 cgtggacatc aaccccgtca gcaagccgat cattgtccct gctttgccgc gcgtcagctc
     1561 gaagacatcg gggtttttct tttgcggcat caagctcgaa cccgtggaga aggcatcgga
     1621 aagctcgaag aaaccgaact cagccgtggt gtacaagaca atttgctcgg caagtttgct
     1681 caagtggaca gacgccatcg aacagacgaa caaatactca gcggcaaaat ccctgtcaga
     1741 gacggcgtcg aggctgttct gcgaaacttc atcgaagcca agagactctg caagggcatc
     1801 gcggtccagc gcaaagggag cgcctgccaa agctccgctt cccaaaggca gaacggatac
     1861 acgtttggtc aggtcttgta atcgttccac atcgcgtttc aacgcccagt agtgactcag
     1921 ccaccaatgc cccaacagga tcggttgcgc tctctgcaag tgggtataac cgggcatgat
     1981 ggtctcaccc gcaagttccg cctgttcgac caacgcattt tgaagaatga ggattgcgga
     2041 atgaagttcg ggaattttgt tcaacatcca caacctgaaa tctgtggcaa cctgatcgtt
     2101 gcggcttcgc ccggtgtgga gtttgccagc agcgggcccg atcagttcgc ttagtctgcg
     2161 ttcaacggcg gtgtggatat cctcgtctga aggttggaag ataaactcac ccgaagcgaa
     2221 ttcatttcgg acggtatcca gcccggttga gatcgaggcg tgttcttcat ccgtaagaat
     2281 gcctgcccgg tgaatcgccg cagcccaggc aatgcttcct tccacatctt gaaatgccat
     2341 gtgttgatcg actggcaggg aggcgttaag cgcccatgcg aggtcatcta gtttttggga
     2401 aaagcgtccg ccccaaaggg tcataaaatc tccttggtaa ttggtaaatg gaaaatggaa
     2461 atttccattt accaattact tttcactaat ctctcttgaa cttcgaataa tccggcttgg
     2521 gcatatcgag accggaaacg ggcaggccgt tgagcgcgcg gactttcatg ctgaggccat
     2581 acaagcgaat gaagccttcg gcatgactct ggtcgtagac atcctcctga ccaaaggtgg
     2641 cgaaatcttc gcgatagagg ctgaacttgg atttgcgacc tgcgctgatg atgttgcctt
     2701 tgtaaagttt gagccgcacg gtgccggtga caggtccctg cgtggagttg acaaaagcat
     2761 cgagcgcttc gcgcagcggt gtgaaccaca gaccgttgta agtgagttcg gcatacttga
     2821 ccgcgatcac ttgtttgtag tgcagggttt cgcggtcgag gatgagcgac tcaagttcgc
     2881 ggtgggcata gagcaggatc gtgccgccgg gggtttcgta cacgccatgt gatttcatac
     2941 ccacgagtcg attctcaacc agatccacgc ggccaatgcc atgatttccg ccaatggcat
     3001 tgagcgtgga aacgatcttc gcaggcgaca tcttctcgcc attgaccgag acagggttgc
     3061 cgctctcgaa ttcaatttcc acatactcac cttgttcggg cgcattctcg ggcgaggtgg
     3121 acatctggaa catcacttct tcgggctcgt tccacgggtc ttcgagcagg ccgccttcgt
     3181 gcgaaagatg ccagagattt gaatcgcgtg agtaaatgga tttaatggtc gcggtcacag
     3241 ggacgttgtg tttcgcggcg tagttgagcg catcttcgcg ggagcggatc tcccactcgc
     3301 gccacggggc aatgatcttc aggcgcggat caagcgccat gacagtcaat tcaaagcgta
     3361 cctgatcgtt gcctttgccg gtcgcgccat gtgcaatcgc atccgcgccg gtctggtgcg
     3421 ccacttctac aagatgtttc gcaattaacg gacgggccac cgatgtgccc aataaatact
     3481 tgtgttcata tactgccccc gctttgagca tcgggaaaag atactcactg acgaattctt
     3541 ctttcatgtc gtgaatgaca aattcgctcg c
//
LOCUS       DAOQLC010000257         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13675_length_3569_cov_25.238190, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000257 DAOQLC010000000
VERSION     DAOQLC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13675_length_3569_cov_25.238190"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..874
                     /locus_tag="PL000_21335"
     CDS             74..874
                     /locus_tag="PL000_21335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91482.1"
                     /translation="MNVNFGLTASDYAKHRAGFPDVFFERMFDEEIVKAGDVLADLGT
                     GTGTLARGFAERGCSVTGIDISAQLLEQAKDFALQAGLEIDFRFAKAEATGLPDSSFD
                     VVTAGQCWHWFDRPSAALEIKRILKPRGRVVIAHFDWLPLNGNVVDLTERLIETYNPK
                     WYVEFGGKTGVYPDWLRDLGEAGFLDIQTFSFDMDVPYTHEDWRGRIRASSGVGASLS
                     EAEVGRFDSELKSMLEGYEQAGAQSEAGSISVLQIPHRVFVVHAHKPN"
     gene            1228..2154
                     /locus_tag="PL000_21340"
     CDS             1228..2154
                     /locus_tag="PL000_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNC91483.1"
                     /translation="MLSIYKTTEQGLEKLETVANGTWVNVADPTPEEIEKLVAWGMDM
                     DYINYSLDQDEMARMERDEDYTFILLRIPMHQPESDVPFNTAPLGIMILGNKIITVCR
                     YDSDIFRVLANGKYRLLKTGKRYRLALYIFLETSARYLNLLREINRATEAVEDQLQKS
                     TRNREVLELLKYQKSLTYFATALRSNEVMMERVQKTQLFNYYEEDQDLLEDVLTENQQ
                     AIQMTSIATEILSGMMDAFASIISNNLNAVMKALAALTIILNMPSLVASFYGMNVALP
                     GEGHPLAFLTVIGLSLALTAIATYIFYKRDWF"
     gene            2156..3010
                     /locus_tag="PL000_21345"
     CDS             2156..3010
                     /locus_tag="PL000_21345"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNC91484.1"
                     /translation="MQTELLLDAKATLGEGPAWDAKTQTLYWLDILEKRVYADTRILA
                     QLDELVGCLAPRKNGNLLLGTRFSFADLAPDSAQLTVLATLSDAERPTNRMNDGKCDP
                     AGRFIAGTMDMNEKDPHGSLYSFDGKQTTRLLNGVTISNGLAWSVDHKTFYYIDTPTH
                     EVKAFDYDLASGQIANPRVAIRIPESLGWPDGMTSDTDGNLWIALWGGAQVTKWNPNT
                     GKLLEQIPVPALQSSACVFGGKNMNELYVTSARVGMSDANLQKYPLSGGLFKIVTGVT
                     GMPTFEFG"
     gene            complement(3060..3488)
                     /locus_tag="PL000_21350"
     CDS             complement(3060..3488)
                     /locus_tag="PL000_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91485.1"
                     /translation="MTVLPESLLEVREHHEPDYKPMVDYQSWRVALMNYTADLLPEKI
                     NRVQKHTETDEVFVLMKGRCILFIGEGEESVTKIHAVDMELYKLYNVKKGVWHSHTFS
                     EDARVLIVENRDTVVENSPFVPLSESQCKEVVELTNKLWG"
BASE COUNT          854 a          879 c          935 g          901 t
ORIGIN      
        1 attttcaagt ttcgaatttg tgagaggccg cttttgcagc ggtctttctt aatggaacgg
       61 agtcgattgc ttcatgaatg tcaactttgg gttaacagca agtgactatg ccaaacatcg
      121 tgcgggattc cccgatgtgt tctttgagcg gatgtttgac gaagagattg tcaaagcggg
      181 ggatgtgctt gcagacctgg gcacaggcac gggcaccctt gcacgcggat ttgccgaacg
      241 cggctgttcg gtgacgggaa ttgatatttc tgcacagctg ctggagcagg cgaaggattt
      301 tgccttgcag gcagggctgg agatcgattt tcgttttgcc aaggcagagg cgacaggttt
      361 acccgattcc tcttttgatg tggtgacggc ggggcagtgc tggcattggt tcgaccgccc
      421 atcagcggcg ctggagataa agcgcattct caaacccagg gggcgtgtgg tcattgcgca
      481 cttcgactgg cttccgctca atggcaatgt ggtggacctc acggaacgat tgatcgaaac
      541 ctataatcca aaatggtatg ttgaatttgg cggaaagacg ggtgtgtacc ccgattggct
      601 gcgcgatctt ggcgaagctg gttttcttga tattcaaacc ttttcattcg atatggatgt
      661 gccctacacc catgaagact ggcgcggacg catccgtgcg agctcggggg tgggggcgag
      721 cttgtcggag gcggaggttg ggagattcga ctcggagtta aagtcgatgc ttgaggggta
      781 tgagcaggcc ggggcgcaga gcgaagcggg ttcaatttcc gttttgcaga tcccgcatcg
      841 tgtatttgtt gtgcatgcac acaaaccaaa ttgattcgca aaaatagaga cttggaacgc
      901 gaatagcgcg aatgggcgaa tttcgcaaat tttcttggat ttattcgccc cattcgcaaa
      961 ttcgcggcat tcgtgttgag gttttcctga ctttgctaca gccatgaatt gattcgttgc
     1021 cacggttgtg caattcaatt aacgggcgta aaatagccca gcgaataacg gtaaggaaat
     1081 catgtatcac acggacgaga gtgacctaag ttcttctgac aataacccgc ccagtatagg
     1141 tgggcggctg atgtttaatt taattccaag catcacgtag gtgccatgcg cctctgcgtg
     1201 atgttaattc acgcaaggag gttgccaatg cttagcatct acaaaaccac ggagcagggg
     1261 ctcgaaaaac ttgaaacggt cgcgaacggc acctgggtta atgtggcaga cccaacgccg
     1321 gaagagatcg aaaaattggt cgcctggggg atggatatgg actatatcaa ttattcgttg
     1381 gatcaggatg aaatggcgcg tatggaaaga gatgaagatt acaccttcat ccttcttcgc
     1441 attcccatgc accagcctga aagcgatgtc ccctttaaca cagcgccgct tggaattatg
     1501 attttgggaa acaagatcat cacggtctgc cgttacgaca gcgatatctt tagagtgctg
     1561 gcaaatggta aatatcggtt gttgaaaacg ggcaaacggt accgcctggc tttgtacatc
     1621 ttcctcgaaa cctccgcgcg ttatttaaac ctgttgcgtg agatcaaccg cgccaccgag
     1681 gcagtggaag accagcttca aaaatccacg cgcaatcgtg aagtgttgga attgctcaaa
     1741 tatcaaaaga gcctcacgta tttcgccact gcgctgcgtt ccaacgaagt gatgatggag
     1801 cgcgtgcaaa aaacgcagtt gttcaattat tacgaagaag accaggacct gctcgaagat
     1861 gtgctgaccg aaaaccagca ggccattcaa atgaccagca ttgccactga gatcctttca
     1921 ggcatgatgg atgcctttgc ttcgatcatc tccaacaact tgaatgccgt catgaaggcg
     1981 ttggccgcgc tcaccatcat cctcaacatg ccttcccttg tggcttcatt ttacggaatg
     2041 aacgtggccc tgcccggcga aggccatccg ctggcattcc ttacagtgat cggtttgtcg
     2101 cttgcgctta ctgccattgc cacgtacatc ttctacaaac gggattggtt ctaacatgca
     2161 aactgagttg ctcctcgatg ccaaagccac gctgggtgaa ggccctgcct gggatgcaaa
     2221 aacgcaaacc ctttattggt tggatattct cgaaaaacgc gtttatgcgg atactcgaat
     2281 ccttgcccaa ttggatgaac ttgtcggatg ccttgccccg cgcaagaacg gaaacctgct
     2341 cctcggcacg cgcttcagct tcgccgacct tgcgcctgat tctgcccaac tgacagttct
     2401 tgccactttg agtgacgcag agcgtcccac caaccgcatg aacgacggca aatgtgatcc
     2461 tgctggccgt ttcatcgcag gcaccatgga catgaatgag aaggatccgc acgggtcgct
     2521 gtattccttc gatggaaaac aaacgacccg cctgctcaat ggtgtgacca tctccaacgg
     2581 ccttgcatgg agcgttgacc ataaaacctt ttactacatc gatacgccca cacatgaggt
     2641 caaggctttt gattacgatc ttgcctctgg gcagatcgcg aacccgcggg ttgcgatccg
     2701 tatcccagag tcacttggct ggcccgatgg catgacctcc gacacagacg gcaatttgtg
     2761 gatcgctctt tggggtgggg cgcaggtgac aaaatggaat ccgaacacgg gcaaattgct
     2821 cgaacagatt cccgtccccg cattacaaag ctccgcatgt gtgttcggcg gcaaaaacat
     2881 gaacgagttg tacgtcacct cggcgcgggt ggggatgagc gatgccaatt tgcaaaaata
     2941 cccgctctcc ggcgggctgt tcaagatcgt tacaggggtg acgggtatgc cgacgtttga
     3001 atttgggtag aaagaggaaa gaagaaagag gaaaaatcct ttcctctttc ttcttaatat
     3061 tatccccaaa gtttatttgt taactccacc acctctttac actgactttc cgacaatggc
     3121 acgaatggag aattttccac gaccgtgtca cggttttcta cgatcaacac tcgggcatct
     3181 tcgctgaagg tatgcgaatg ccaaactccc tttttcacgt tgtacagttt gtatagctcc
     3241 atatccaccg cgtggatttt ggtcacactt tcctcccctt caccgataaa taagatgcag
     3301 cggcctttca tcaacacgaa gacctcgtcg gtttcagtgt gtttttgcac acggttgatc
     3361 ttttccggga gcaggtcggc agtgtagttc atcaatgcca cgcgccatga ttggtaatct
     3421 accattggct tgtaatctgg ctcgtggtgc tcgcggactt cgagcaggga ttcgggtaat
     3481 acggtcattg gtctctccat gtttggtctc tttcatttga cctcttgcat aagtaaagtg
     3541 ttgtagacca caaagtcacc aaggcacta
//
LOCUS       DAOQLC010000258         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_13822_length_3545_cov_24.546418, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000258 DAOQLC010000000
VERSION     DAOQLC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13822_length_3545_cov_24.546418"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..1210)
                     /locus_tag="PL000_21355"
     CDS             complement(242..1210)
                     /locus_tag="PL000_21355"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC91486.1"
                     /translation="MRVEVMEYYGLTQPFSQAGYYETQQHTQLLKDIKGAILEGRLIA
                     VCGVVGSGKTVTMRRLQQQLKEENRITVSKSLSVEKHSIKLATLITALYYDLGTDKIQ
                     IPKQGERRERELRELVRKNKRPVALFVDEAHDLNRHTLTGLKRLMEVIEDGDGRLSVI
                     LSGHPKLRNDLRRPTMEEIGFRTDVFNLEGIAGSQREYLHWLLNTSMRDGNSFESVLT
                     LDAIDLLASRLRTPLQIQLHLTMSLEAGYQTGEIPVSAELVESVLSRQLDDLEPTLTR
                     HGYHLKDLVEQFDVKPAEIKALFNHQLDTTRVTELRRQFLAAGLPI"
     gene            complement(1200..2837)
                     /locus_tag="PL000_21360"
     CDS             complement(1200..2837)
                     /locus_tag="PL000_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HNC91487.1"
                     /translation="MPTDSLLQLQQRLDRLPRKSAERVVQITSMAELYGVSPTTVYRA
                     LKRCLKPRAAHRADHGQPRLLPKPELEHYCELVAALKLRTTNKKGRHLSTQRAIELLE
                     QHGIETAHGLVKAPQGLLHKSTVDIYLRQLHLDQGRLQREPPAVRFQALHSNDCWQFD
                     MSPSDLKHIDKPDWIDPTKGMPTLMLFSVVDDRSGVNYQEYRCVYGEDAESALRFLFN
                     AMTPKADASFLFQGRPKLIYLDNGPVAKSRVFQNVMLALGIEWLTHLPAGSDGSRTTA
                     RSKGKVERAFRTVKDAYETLYHFHKPETEQQANAWLHRYLIHDYNLRQHRTEHHTRLD
                     DWLANLPTEGIREMCAWEQFCRFAREPERRKVGVDARISIDGTAYEVDPAMAGETVIL
                     LWGLFDSELYIEFEGDRSGPYYPISGPIPLHRYRAFKRGKADERADRIRALADQIGLP
                     IAALSGDDVRLTPTTGTIAIPKQPFDAQSLEYQFPHVIAAKLAIADDLARPLAKLSSA
                     ERAFIDQVLAETLIRNTVLSRVRHYFRHQTGGEDHAS"
     gene            complement(2842..3417)
                     /locus_tag="PL000_21365"
     CDS             complement(2842..3417)
                     /locus_tag="PL000_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC91488.1"
                     /translation="MIIGYARVSTDDQHLDLQRDALQKAGCERLFEDKASGVKANRTG
                     LGQLLMSLRAGDTVVVWRLDRLGRSFKDLIAMIEQFDHAHVGLRSVQENIDTTSIGGR
                     LVFHLFGALAEFERHLIRERTQAGLSAARARGRQGGRKKRLDPTKRAVALRLYHERNH
                     TVEEICRMMGVGRSTLYNYLTEAERDVLQSP"
BASE COUNT          927 a          871 c          815 g          932 t
ORIGIN      
        1 acagtaactt tataaaatct aactttcatc gtgcctgatt ctagattaaa ttatctgtag
       61 cgacttgcat ttgaaaatgc tcaggcctgc attcaacgca ctttgaggcc agaactaagt
      121 gcatttatcg gatgtctcag gacaggatta attgccactg agcctgcatt acattttcag
      181 gactgcattt catcgcgtca ggacagcatt aactgcaaat gaaaatagtg acgatagcgc
      241 gtcaaatagg caagcctgca gccagaaatt gacggcgtaa ttctgtaaca cgagtggtgt
      301 ctaattgatg attaaacaag gccttgattt cggcaggttt gacatcaaac tgttccacga
      361 gatctttcaa gtggtagcca tgacgcgtta atgtcggctc cagatcgtcc aactgccgtg
      421 acaacacgga ctcgaccaat tcagcagaaa ctggtatttc ccctgtttga taacctgctt
      481 ctaatgacat cgtgaggtgt agttggattt gtagtggtgt gcgtaaacgg gaagctaaca
      541 agtcaatagc atctagcgtc aagaccgatt caaaagagtt gccatcacgc atagaagtat
      601 tgagcaacca gtgtaaatat tcacgttgac tgcctgcgat accctccaaa ttaaatacat
      661 cggttcgaaa gccgatttcc tccatggtgg gacgacgtaa atcgttacgc aatttaggat
      721 gacctgaaag aatcactgac aatcgcccat caccatcctc aatcacttcc atcaaacgct
      781 ttaatcctgt cagcgtatgc cgattcaagt cgtgcgcttc gtcaacaaac aaggcaacag
      841 gacgtttatt cttgcgtact aactcccgta actcacgttc acgccgctcc ccctgtttgg
      901 gaatttgtat tttatcagta ccaagatcgt aatacagtgc agtaatcaga gtggcgagct
      961 tgatactgtg cttttccacc gacaaagatt tggagaccgt aatgcgattc tcttccttca
     1021 actgttgctg taaccgcctc atggtcacgg ttttgccgct gccgactaca ccacacactg
     1081 caatgaggcg accctctaaa attgcccctt taatgtcttt gagcaattga gtatgctgct
     1141 gcgtttcata gtaacccgct tggctgaatg gctgcgttag cccgtaatac tccatcactt
     1201 caactcgcat gatcttctcc tcctgtttga tgacggaaat aatggcgcac gcgtgatagt
     1261 acggtgttgc gtatgagcgt ttctgctaag acctgatcaa taaaggcacg ttctgctgat
     1321 gacaacttcg ccaatggacg agcaaggtca tcggcaatcg cgagtttggc tgcaatcaca
     1381 tgaggaaatt ggtattccaa tgactgggca tcaaagggct gctttggtat cgctatggtt
     1441 cccgtggtcg gtgttaagcg cacatcatca cctgacaatg ccgctatggg taagcctatt
     1501 tgatcggcta aggcacgaat acggtcagca cgttcgtctg ctttacctcg tttgaacgca
     1561 cgataacgat gtagaggaat agggcctgat attggataat aaggccccga acgatcaccc
     1621 tcaaactcga tataaagttc gctatcaaac aaaccccaca gcaagataac cgtttcacca
     1681 gccatcgcgg gatccacttc atacgctgtc ccgtcaatgg agatgcgtgc atcgacaccg
     1741 actttacgcc gttcaggctc gcgggcaaaa cggcaaaatt gctcccatgc acacatttca
     1801 cgaatccctt ccgtgggtaa attggctaac caatcgtcta aacgagtatg atgctcggtg
     1861 cgatgttggc gtaggttgta atcatgaatc aaataacggt gtagccatgc gtttgcttgt
     1921 tgttctgttt caggcttatg aaagtggtat aacgtttcat aggcatcttt cactgtacga
     1981 aaggcgcgtt cgaccttgcc tttggagcgt gccgtagtac gagaaccgtc tgagcctgcg
     2041 ggaaggtgtg tcagccattc aataccaagt gccaacataa cattctgaaa aacacgactc
     2101 ttggccacag ggccattatc caaataaatc agtttggggc gaccctgaaa aagaaagctg
     2161 gcatccgctt tgggtgtcat cgcgttaaat aaaaagcgta aggctgactc tgcgtcctcc
     2221 ccgtaaacac aacgatattc ctgatagtta accccactac gatcgtcgac cacgctaaat
     2281 aacatcagcg ttggcatccc tttcgtggga tctatccagt cgggtttgtc gatatgcttg
     2341 agatccgaag gtgacatatc aaattgccag caatcattgc tatgtagagc ttgaaaacgg
     2401 acggcaggtg gctctcgctg tagtcggcct tgatccaaat gcaattgcct cagataaata
     2461 tcgacagtgc ttttatgcag taagccttgt ggggctttga caaggccgtg agccgtttca
     2521 atgccgtgct gctctaataa ctcaatagca cgctgcgtgg acagatgtcg gcctttttta
     2581 ttcgtggtac gcagtttaag cgcagcgacc agttcacagt aatgttccag ttcaggtttt
     2641 ggcagcaggc gaggttggcc gtgatcggct cgatgtgccg cccgaggttt tagacaacgc
     2701 ttgagcgcac ggtagactgt tgtcggtgat accccataca gctcagccat tgatgtaatc
     2761 tggacgacac gttcggcgct cttacgcggc agccgatcca gtcgttgctg taactgcaac
     2821 aacgaatccg taggaatctc tttacggcga ttggaggaca tcacgttccg cttccgtcag
     2881 gtagttgtat agcgtggaac gtccaacacc catcatccga caaatttcct ctaccgtatg
     2941 atttctttcg tgataaagcc gtaaagcaac ggcacgcttg gtggggtcaa gacgtttctt
     3001 gcgcccacct tgtcgaccac gcgctcttgc cgcagacaag cctgcttgcg tgcgctcacg
     3061 aatcaaatgg cgttcaaatt cggctaatgc accgaataag tgaaaaacca accgtccacc
     3121 aatggatgtg gtatcaatat tttcctgtac actgcgcaga ccaacatggg catggtcaaa
     3181 ctgttcaatc atggcgatga gatctttaaa agaacgcccc agcctgtcta gtcgccaaac
     3241 aactacggtg tcgcccgcgc gtagactcat caataattga ccaagccctg tacgatttgc
     3301 tttgacacca ctggctttat cttcaaaaag acgttcgcaa cctgctttct gcaatgcgtc
     3361 tcgttgcaga tctaaatgtt ggtcatctgt tgaaacacga gcatagccga taatcatgat
     3421 gcgtccagat ttcacgtcaa acttaagtgt agttggatat tgaaaaaagg agtgggaatt
     3481 tggacggttt ttcgagggta tttctacaga aatggactat tttcaaaatg accatcaaac
     3541 catcc
//
LOCUS       DAOQLC010000259         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_14326_length_3470_cov_26.387994, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000259 DAOQLC010000000
VERSION     DAOQLC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14326_length_3470_cov_26.387994"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..544)
                     /locus_tag="PL000_21370"
     CDS             complement(215..544)
                     /locus_tag="PL000_21370"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HNC91489.1"
                     /translation="MNFNDYQTKSRVTAKYPAIGHGVVYPTLGLVNEAGEVAGKIKKV
                     FRDKSGEISAETREALKAELGDVLWYLAQTCTELEISLDEVAEANLAKLLDRQARGKI
                     QGDGDNR"
     gene            complement(720..1250)
                     /locus_tag="PL000_21375"
     CDS             complement(720..1250)
                     /locus_tag="PL000_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91490.1"
                     /translation="MSLIQETKPAQRKPTPVILTVFLIIFLAATAGLGYWAYTLNSSL
                     IATQQQLAELQSDHTKLQSENENLTAELAQVKADLEESSKDLTNTQTKLEKAQAENLE
                     LNVKIENASKKLQILYAFSTIATPTDILAIDTLIKETKDRQLLERWNEFYSNPSESAG
                     VNFILNLITAIQTSLK"
     gene            complement(1447..2352)
                     /locus_tag="PL000_21380"
     CDS             complement(1447..2352)
                     /locus_tag="PL000_21380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91491.1"
                     /translation="MKITSRLYEGPNDFQTMLNLLAKIRPAARMNDFPVKVDIEENMA
                     NEEIRANTRLWFEGDRPIGWAYVDDGNNLRWEVEKQFEDSVGAEMVVWGEACMRSRRK
                     LNTGETSTLDASCREENHERLEFIRKHGFHQTDVISVGMKRLLSEPIPEPELPHGFSI
                     RAVKGTEEAEAVASTHRSAFGTEYMTTENRLIIMNSSEYDPSLDLVVIAPDGSIAAYC
                     TCSVNANGEGYTDPVATHPRYQRLGLAKALLLKGMQLLKERGMKSASLGTNGDNIAMQ
                     RTAEAVGFRLEHKTLWFEKEVNQID"
     gene            complement(2429..>3470)
                     /locus_tag="PL000_21385"
     CDS             complement(2429..>3470)
                     /locus_tag="PL000_21385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HNC91492.1"
                     /translation="RETMKLDAALPIVGLKDIPAIAEAAQAIGFDALWTQETQHDPFL
                     PCTLIAEHSETLRFGTAIAVSFSRSPANLAYTAWDLAAQSNGRFILGLGTQVKAHIER
                     RFGMAWPESVTGKLREQIQVIRAFWDCWQNGTKLNFRGEYYKATLMSPFFQPPPLPSP
                     IFGENGGRLGGGIPIYIAGVNAGLAKLAGELCEGFHAHPFNSSRYMNEVMLPAIEEGL
                     KKEGRQRQDITVSMTPFIATSPEEESFARMQIAFYASTPSYKSVMDFYGWGATAEKLS
                     GFAAKGEWAEMPMLITDDMLNEFCLMTTQENLAVDLKKRYAGIADRLTLYTPFVPGEK
                     DDWWRNLAKAFN"
BASE COUNT          771 a          877 c          834 g          988 t
ORIGIN      
        1 accaaagaaa aaagcggaca aggacaggag cagaatcact ccccgcatgg tcaccctgct
       61 tggaaaccct cttgcaaaaa ggtagaacgc catccacagg cagatcgcca ttgcggtgat
      121 gtcaaccaat accgtcgctt gaaatagcat ttcttctcct tgcctgtaca aaccaatgga
      181 gaacagtgtt gccgtgtatc agaaccctga cactttaacg attatcccca tcaccctgta
      241 tttttccgcg tgcctgcctg tctaaaagtt tggctaaatt ggcttctgcc acttcatcaa
      301 gggaaatttc cagttcggtg caggtttggg cgagatacca gagtacgtcg ccaagctcgg
      361 ctttgagtgc ttcgcgcgtt tcagcgctga tctctccgct tttatcgcgg aatacttttt
      421 tgatcttgcc cgccacttca cccgcttcgt tgaccaaccc cagagtgggg tagacaacgc
      481 cgtgcccaat ggcagggtat tttgcagtga cacgtgattt ggtctggtaa tcgttgaagt
      541 tcatgagttt tccttcagta tgtttttttg gataaatttc tcacgaattg gattttacat
      601 tacttttgaa tagtcatttc ctttgcctga atgcaaaatg ttgcaggttg cggaaaaaat
      661 aaaagccctg tacctgttct tcagatagta caaggctctt cgtgttcaga gtggtggaat
      721 tatttcaggc tggtttgtat tgcggttatc aagtttagaa taaaattgac accggcagat
      781 tcggatgggt ttgagtaaaa ctcattccaa cgttcgagta gctgcctgtc ttttgtctct
      841 ttgatcagcg tgtctattgc cagaatgtct gtaggggtcg cgatagtgga gaatgcatac
      901 aggatttgca gtttcttgct ggcattttcg atcttcacat taagttcaag gttttcggcc
      961 tgcgcttttt ctagcttggt ttgcgtgttg gtcaggtctt tactgctttc ttccaggtca
     1021 gccttgacct gggcgagttc tgctgtcaga ttttcgtttt cactttggag ttttgtgtga
     1081 tctgactgta attcagccaa ttgctgttgt gttgcgataa gacttgagtt taatgtgtaa
     1141 gcccagtaac caagcccggc cgttgctgcc agaaaaatga tcagaaatac cgtcaggata
     1201 acgggggttg gttttctttg tgcgggcttt gtctcctgaa tcagagacat tcctggctgt
     1261 tctatttcct tttgagaaaa tgattcgggt tgcatgctat cctccatatt tgtgcaattt
     1321 gatttaaatt atttttgctg gtgcttttgg gatggccaat ccctaactag aaaatacccc
     1381 ggcatgatga agccattata ctaattccat acttaaagtc aactagatat tgcggatctt
     1441 ctactttcaa tctatttggt tcacttcctt ttcaaaccac aacgttttat gttccagacg
     1501 aaacccaacc gcctcggctg tccgttgcat ggcgatattg tctccattag tgccgaggct
     1561 cgcggatttc atgccgcgtt ctttgagcag ctgcattcct ttaagcagga gcgcctttgc
     1621 caaccccagc cgttgatagc gcggatgtgt ggccacgggg tcggtgtatc cctccccgtt
     1681 cgcattgacc gaacaggtgc aatatgcggc gatgctgcca tcgggtgcaa taaccacaag
     1741 gtcaagcgac gggtcgtatt cgcttgaatt catgatgatc aggcggtttt ccgtggtcat
     1801 gtattccgta ccaaatgcgg atcggtgcgt gctggcaact gcttctgctt cctccgtccc
     1861 ttttacagcg cggatggaga atccgtgcgg cagttcaggt tcgggaatgg gttctgaaag
     1921 caggcgtttc atgccgaccg agatcacatc tgtttgatgg aatccgtgct tgcggataaa
     1981 ttccagtcgt tcgtgatttt cttccctgca acttgcatcc agtgtggatg tttcccccgt
     2041 gttcaatttc ctgcggcttc gcatgcaggc ttcgccccat acaaccattt ccgcgccgac
     2101 ggagtcttcg aattgttttt ccacttccca gcgcaggttg ttcccatcat ccacataagc
     2161 ccaaccgatg ggacgatctc cttcgaacca caaccgtgta ttcgcacgga tttcttcatt
     2221 cgccatgttt tcttcgatat ccaccttgac ggggaagtca ttcatccgtg cagcgggacg
     2281 aatctttgcc agcaggttga gcatggtttg aaagtcgttt gggccctcat agagtcgact
     2341 ggtgattttc aagctggact cctttataaa aacctggagc gcggacttta gtccgctgtg
     2401 tggcgggcag actaaagtcc acgctctgct aattaaacgc cttcgccaga ttcctccacc
     2461 aatcatcctt ttcaccgggg acaaagggtg tgtagagcgt caggcgatct gcgatacccg
     2521 cgtaacgctt cttcaaatca acagccagat tttcctgtgt ggtcatcaga cagaattcat
     2581 tcagcatgtc atcggtgatg agcatgggca tttcggccca ctcgcctttg gcggcaaacc
     2641 ccgaaagttt ttcggcggtt gcgccccagc cataaaaatc catcacagat ttataagacg
     2701 gtgtgctcgc gtagaaagcg atctgcatcc gtgcgaaact ttcttcctcg ggtgatgtcg
     2761 caataaacgg agtcatcgaa acggtgatgt cctgtcgttg gcggccttct ttttttagac
     2821 cttcttcaat cgcaggcagc atcacttcat tcatgtagcg cgaactattg aacgggtgcg
     2881 cgtggaatcc ctcgcatagt tcgcccgcca gttttgccag ccctgcattc acgcctgcaa
     2941 tataaattgg aataccccca cccaaccttc ccccattttc cccgaaaata ggggaaggca
     3001 agggaggggg ttgaaaaaac ggcgacatca acgtggcttt gtaatactcg ccgcggaaat
     3061 tcaacttcgt tccattttgc cagcaatccc aaaaagcgcg gatgacttga atctgctcgc
     3121 gcaattttcc cgtcaccgat tcaggccagg ccatgccgaa acgccgctca atatgcgcct
     3181 tgacctgtgt gcccaacccc aaaataaacc gtccatttga ctgtgccgcc aaatcccatg
     3241 cggtgtaggc aaggttggcg ggcgagcgcg aaaacgagac cgcgatggcg gtgccaaagc
     3301 gcagagtctc tgaatgttca gcgatcagcg tacagggcag aaaaggatcg tgctgcgttt
     3361 cctgagtcca caacgcatcg aatccaattg cttgtgcggc ttcggcgatc gcaggaatat
     3421 ccttcaggcc aacaatgggt aatgctgcat cgagtttcat ggtttccctt
//
LOCUS       DAOQLC010000260         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_14562_length_3439_cov_20.692080, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000260 DAOQLC010000000
VERSION     DAOQLC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14562_length_3439_cov_20.692080"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1201
                     /locus_tag="PL000_21390"
     CDS             107..1201
                     /locus_tag="PL000_21390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC91493.1"
                     /translation="MNFIKRKFFKLHLVVLIGIMLLSACKGTTAQPADVPPDQQTGEL
                     TVLDWAGYDAPDFWVDFQTTYPKVTVNFETGESDADIYAKMKAGDQADIFHPYTGWLQ
                     FYVDEGLVEEIDTSKLSNWDKVPENFKKIGQINGKQYFIPWDWGFSSILYRTDKIPEG
                     VDSWSAFFDPKYAGHVSMWDDGTASVLVSSYIHEYDITNLTDAQQASIKDEWTAQRDL
                     NLFYWAGEPELVQAMSSGDVWLAYGWQGAYATLLSQGVPVAYATPKEGRNSWVGLYGI
                     RKGTENYDLALKFLDAKLGNATSNNLINLFYYGASNQEVMSGITDPTLMQAFSINDPS
                     ILQHTNFTPNLTAEQRDAWTRMWAEIKAAP"
     gene            1198..2130
                     /locus_tag="PL000_21395"
     CDS             1198..2130
                     /locus_tag="PL000_21395"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC91494.1"
                     /translation="MSFDPTSQQNNDLTRVPPNPGKRGRKLDRQSTFWFLYLPPLVWL
                     GLFFLAPLLLMAAFSFRADSYGSLFENWVPGFDQYASVLSTAGYWRLLGISTSTALIV
                     SISAVVLAYPLAYFLAYYSGKRAGLYLLLLMVPFWTSYLLRVMAWKLMLGTDGVLNSL
                     LLYLGWIKEPLTALLYNRFAVTLTLIYVWVPFASLPILSALQRIDWSLFEAAADLGAR
                     PYHQFMRITLPLSLPGVLAAFFMVFIPTVGEYVTPLLVGGTRGILYGNIIQDFFTKAA
                     NWPLGSALSMVMLAVTLVLVFLATRLVKLEDVLQ"
     gene            2127..2909
                     /locus_tag="PL000_21400"
     CDS             2127..2909
                     /locus_tag="PL000_21400"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC91495.1"
                     /translation="MNERRAPFPLSLYYFSLIVLLYLPIGILLLFSLNSNTTLTFPLR
                     GLTFDWYRNLLTSDAVLRSARNSLIVASGSSLVATVLGTMVSLLLTRFQFKGKNLLLM
                     LSALPLIVPYIVLGVALLLLFSALHIERSLFTVTIAHTVVALPYTLLIISSRLSGFDT
                     SMEEAAMDLGADYPTTLRRVVLPMIFPAMVSAWLTAFTVSFDEFALALFLSGTQPTFP
                     VYLFSQLRFANRLPLMIALAVLLMIGTLTLVFIAEKYRQKES"
     gene            2959..>3439
                     /locus_tag="PL000_21405"
     CDS             2959..>3439
                     /locus_tag="PL000_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91496.1"
                     /translation="MADSDLSTLCKACGLCCSGHLFSWVRLNAPELDTVQSLGVAVIR
                     NDPRQRGFTQPCPLWDGVCTVYTSPHYPRSCKTYKCKVFKQLLDEDVLLSDAMTVVQD
                     TLNLIREIEPLLPESTTISFRERVIAYKEELETKKGGHTTAEQEFLSKTAVLLTRYED
                     "
BASE COUNT          725 a          887 c          825 g         1002 t
ORIGIN      
        1 gggcatgacc aatttcccat gcctttcccc tctatttaga gaggttgcaa atcttataag
       61 cgaacataaa atcctcccca tcaacaagaa taaaaaggag aattttatga atttcatcaa
      121 acgtaagttt ttcaaacttc acctggtggt gttgatcggc atcatgctgt tatctgcctg
      181 caaaggcact actgcccaac ccgctgacgt accgccagat caacagaccg gtgagctgac
      241 cgttttggat tgggcgggtt acgatgcgcc cgatttttgg gtcgattttc aaaccaccta
      301 ccccaaagtg acggtcaatt ttgagaccgg tgagtcagat gccgatattt atgccaagat
      361 gaaggctgga gatcaggcag atattttcca tccatacact ggctggctgc aattttatgt
      421 agatgaaggc ttggtggaag agatcgatac aagcaaatta tccaattggg ataaggtccc
      481 cgaaaacttt aagaagatcg ggcagatcaa cggcaagcaa tatttcatcc catgggattg
      541 gggttttagc tcgatcctct atcgcacaga taaaattccc gaaggtgtgg attcgtggtc
      601 ggcttttttt gacccgaagt acgctggtca tgtctccatg tgggatgatg gaaccgcctc
      661 cgttcttgta tcctcctata ttcacgagta cgacataacg aatctgaccg acgcccagca
      721 ggcctctatc aaggatgaat ggactgccca gcgcgatctc aatctgtttt actgggcggg
      781 cgaacctgaa ttggtgcagg cgatgtctag cggtgatgtt tggcttgctt atggctggca
      841 gggagcatat gccactttat taagccaggg tgttcctgtg gcatatgcca ccccgaagga
      901 gggccgcaac tcatgggttg gtttgtatgg catccgcaag ggaaccgaaa actacgatct
      961 ggcgctgaaa ttcctcgatg caaaacttgg caatgccacc agtaacaacc tgattaatct
     1021 tttctattat ggagcttcga atcaggaagt gatgagcggc atcaccgatc caacgcttat
     1081 gcaggccttc tccattaacg acccatccat tctgcagcat acaaacttca caccaaacct
     1141 tacagcagag cagcgcgatg cctggaccag gatgtgggct gaaatcaaag ctgccccatg
     1201 agttttgatc ccacctccca acaaaacaat gaccttaccc gggttccacc gaacccgggt
     1261 aagagaggaa gaaaactgga tcgccagtcc acgttttggt ttttgtacct tccgccttta
     1321 gtttggctcg gcctgttctt tctggctccg ctcctgttga tggcggcttt tagttttcgt
     1381 gctgattcct acggatcgct gtttgagaat tgggttccgg gcttcgatca atacgcttca
     1441 gtgctttcta ctgcaggcta ttggcggctc ctgggaattt caacttcaac cgcattgatt
     1501 gtttcgatca gtgccgttgt gttggcgtac ccgctggcat attttcttgc ttattattct
     1561 gggaaacgag cgggtctgta tcttctttta ttgatggttc cgttttggac gagttatctc
     1621 ctgcgggtga tggcatggaa actcatgctt ggcaccgacg gtgtgttgaa ttcattgctt
     1681 ctctacctcg ggtggatcaa agagccatta accgctctgc tctacaaccg ttttgcggtc
     1741 accctcactc tgatttatgt atgggtgccg tttgcttccc tgcccatcct ttctgccttg
     1801 cagcgcatcg actggtcgct gtttgaagcg gccgccgatt tgggggcacg cccctatcat
     1861 cagttcatgc ggatcaccct tccgctcagc cttcccgggg ttttggctgc gttctttatg
     1921 gtcttcatcc caaccgttgg cgaatacgtt actccgcttt tggtgggcgg aacacgcggc
     1981 atcttgtatg gaaatatcat tcaggatttc ttcaccaagg ctgcaaattg gccgttgggc
     2041 tctgcccttt ccatggtcat gcttgctgtt actctcgttt tggtcttcct tgccacacgc
     2101 cttgtgaagc tggaggatgt tttgcaatga atgaacggcg cgcacctttt ccactttccc
     2161 tatattattt cagcctgatt gttttgcttt atttgccaat tggcattctt ttgctgttct
     2221 ctcttaactc aaacacaaca ttaacctttc cgcttcgcgg tttgaccttt gactggtatc
     2281 gaaatttatt gacatccgat gcggtgctgc gctcagcgcg taacagtttg atcgtggcgt
     2341 ctggcagcag tctggtcgcc accgtccttg gaacgatggt gtcgctcctt ttgacgcgtt
     2401 ttcaattcaa aggtaagaac ctgcttctga tgctgtctgc cctgccgttg atcgtccctt
     2461 acatcgttct cggtgttgcg cttctattgt tgttttctgc cctccatatc gagcgttccc
     2521 tgttcacagt gacgattgcc catacagtgg ttgcactgcc atacaccttg ttgatcatct
     2581 cttcacgtct atctgggttt gacaccagca tggaggaagc cgccatggat ctgggcgcag
     2641 attatccaac cacgcttcgc cgcgttgtcc tgcccatgat tttcccagcc atggtctctg
     2701 cctggctgac cgctttcaca gtttcttttg atgaatttgc cctggccttg tttctttctg
     2761 gcacacaacc aaccttcccc gtctatctgt tcagccagtt acgatttgcg aatcgactcc
     2821 ccctgatgat tgcccttgcc gttctgttga tgatcggcac cttgactttg gtcttcatcg
     2881 ccgaaaaata tcgccaaaaa gaatcttgat cgtgtttttc atctgggatt tggccataac
     2941 ttttgttatc atctgggcat ggccgattct gatttatcaa ctctctgcaa agcgtgcgga
     3001 ctgtgctgtt cggggcatct tttttcctgg gtgcggctca atgctccaga attggacacg
     3061 gtgcaatccc tcggtgtggc cgtgatccgc aatgacccgc gtcagcgcgg atttacgcag
     3121 ccctgtccgc tttgggatgg ggtttgcacg gtctacacct cgccgcatta tccgcgcagc
     3181 tgcaagacgt ataaatgcaa agtcttcaag caattgctgg atgaggatgt tttgctttct
     3241 gatgcaatga cggttgttca agataccctg aacctgatcc gcgaaatcga gccgcttctc
     3301 cccgaatcaa cgacgattag ctttcgtgag cgggtcatcg cttacaaaga ggaattggaa
     3361 acaaaaaaag gcgggcacac aacagcggaa caggaatttt tgagcaagac cgcggtgctg
     3421 ttgacccgtt acgaagatc
//
LOCUS       DAOQLC010000261         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_15822_length_3275_cov_28.000000, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000261 DAOQLC010000000
VERSION     DAOQLC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15822_length_3275_cov_28.000000"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..2558
                     /locus_tag="PL000_21410"
     CDS             153..2558
                     /locus_tag="PL000_21410"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNC91497.1"
                     /translation="MNALVKLADREMAVETSMEVSNRVERLKKAVQAETNGACIERPM
                     LWTKYHRNKANSDKTTNVKIAEAVRYVLNNKTVRIYDDELIVGNFTSKRVAGFCYPEL
                     SGLEPMSQVFNMPSRKVNPLEISLKDRFKLFSQIPFWLNKNTSTLAFDGMKEGMRFAK
                     EQSEALQYQIYEAGGISHIAPNYEKIINVGAQGIIEEVEALESKTDDSEKLDFYKAVK
                     ISMKGMAEFGDRYAAEASKMASVETDPVRKQELLEIASICARVPRFGATSFYEAVQSM
                     TLAHICIFMETLGETTCPGRIDQFLNPFYEKDVAAGNISRERAKEILGAFCVKLCETI
                     PMHGEVGTSTLGGLTSWEVVTIGGQDKDGNDATNELSFILLELADELRMRQPNFHVRI
                     HKNTPKAFYDEVIRINFGPGSAPAMYNDEIIIESMTDIGYSLEDARNYVAIGCVEPTA
                     PGKTLASTDAAMYNMPLAIELLLNEGRQFGSKKQIGAKTPPVSEMKTMDDVVAAYDTQ
                     VKHQLGKLRRDLEAVEKFHAKHHPTPLSSALLEGCVETGVCSTKGGAVYNFSGIQGCG
                     MAATADSLRCIEKAVFEDGWISLEGLVDQLKDNISDEKVFTKLNAIDKFGNDIPEADK
                     WMIWVGEHYADNIKALGKNTRGGNHNAGVYSNTSHTHFGGLVNALPNGRRAGETFASG
                     FAPENGADKRGTTALLNSMNKINYKNFANGINFNIKLDASSYECDDGKSALGSLYKVF
                     FKREGMQVQANMLDPKILIEARDNPELHPNLLIRVSGYSAYFNDLSPKMQDEVISRSS
                     HAA"
     gene            2548..>3275
                     /locus_tag="PL000_21415"
     CDS             2548..>3275
                     /locus_tag="PL000_21415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNC91498.1"
                     /translation="MRPDASLINSINVTEPRKASLYFDVKRNTLDDGPGIRTVVFFKG
                     CPLSCTWCQNPESINTGYELSFNAEKCIECGDCVSACPEQAIDLTVPGHILRDKCTQC
                     FKCVDVCPSGALEQIGRPTNDLEALAEEVLEDADFFEMSGGGLTVSGGEALMQMDSVG
                     ELFQLVKRKRDIHTLIQTAGLFNFSRFESLVLPHTNTIYFDLKLINSDDHLTYCGVKN
                     EPIIENFKKVQALALTTDLEVLPRV"
BASE COUNT          868 a          733 c          828 g          846 t
ORIGIN      
        1 ggccttgatc cgggatttcc cttaaaaagg cacatcaact ataagctgat ttgcatgtat
       61 tttgtcgtca gctaagtaga aagtgatagc gtcgagaacg gatccggtgt gattgtcggt
      121 tctgtaactg gggtgaatac taggagaata agatgaatgc tcttgtgaaa ttagctgata
      181 gagaaatggc ggtcgagact tctatggaag tatcgaaccg tgttgagcgc ctgaaaaaag
      241 cagtgcaagc cgaaaccaat ggtgcatgca ttgagcgtcc tatgctttgg accaaatacc
      301 atcgtaacaa ggccaacagc gataagacga ccaacgtgaa aatagccgaa gctgtgcgct
      361 atgtgctcaa taacaagact gtgcgtattt acgatgatga gttgattgtt ggcaacttca
      421 cctccaaacg cgtcgctggc ttctgctatc cagagttgag tggcttggaa cccatgtccc
      481 aagtattcaa tatgccttct cgaaaggtaa atcccctcga aatcagtttg aaagaccgct
      541 ttaagctatt ttcacagatt cccttctggc taaacaaaaa tacatcgact ctggcgttcg
      601 acggcatgaa agaaggaatg cgtttcgcca aagaacagtc ggaagcctta cagtaccaaa
      661 tctatgaagc tggtggtatc tctcatatcg ctcctaacta cgagaaaata atcaacgttg
      721 gtgctcaagg aattattgaa gaagtagaag cacttgagag caaaaccgat gattctgaaa
      781 aattggattt ctacaaggcc gtcaaaattt ctatgaaggg tatggcggaa ttcggtgatc
      841 gctacgccgc tgaagccagc aaaatggcct ctgttgaaac cgatccggta agaaagcaag
      901 agctgttgga gattgcttcc atttgtgccc gtgtacctcg ctttggtgcc accagcttct
      961 acgaagcggt tcagtccatg acgctggcac acatctgtat ctttatggaa acccttggtg
     1021 aaactacatg cccaggtcgt atcgatcagt tcctaaatcc gttctatgag aaagatgtgg
     1081 ccgctggcaa tatttctcgt gagagagcta aagaaatcct cggcgcgttc tgcgtcaaac
     1141 tgtgtgaaac cattccaatg cacggtgaag tcggtaccag cactctgggc ggtttgacca
     1201 gttgggaagt tgtgactatc ggcggtcagg ataaagacgg caacgacgcc accaacgagc
     1261 tgagctttat attgctggaa ttagccgatg agctgcgtat gcgtcagcct aactttcatg
     1321 tgcgtattca taaaaatacc cccaaagcgt tctacgacga ggtgattcgc attaacttcg
     1381 gccctggttc tgctcctgcc atgtacaacg atgagatcat tatcgagtcc atgaccgata
     1441 tcggttattc tctggaagac gcacgcaact acgtggctat tggctgtgta gagcctaccg
     1501 cgccaggcaa aactctggct tccactgatg ccgctatgta caacatgccg ttggcgattg
     1561 aattgctcct taatgaaggt cgtcagtttg gttctaaaaa gcaaattggt gctaaaaccc
     1621 ctccagtgtc cgaaatgaaa accatggacg atgtggttgc ggcttacgat acccaagtga
     1681 agcatcagct gggtaaactg cgcagagacc tggaagcggt agaaaaattc catgccaagc
     1741 atcatcccac cccgctcagc agtgcgctgc tggagggatg tgtggaaaca ggtgtttgct
     1801 ctaccaaagg tggcgctgtc tataatttct ctggtattca gggctgtggt atggcggcca
     1861 ctgcggattc attgcgctgc attgaaaaag cagtatttga agatggttgg atttccctgg
     1921 aagggctggt cgatcagctg aaagacaata tcagcgatga gaaggtgttc actaaactaa
     1981 atgccattga taaattcggt aacgatattc cggaagccga taagtggatg atctgggttg
     2041 gtgagcacta cgctgataac attaaggcac tgggtaaaaa tacccgtggc ggcaaccaca
     2101 atgccggtgt ttattccaat acgtcacaca cgcacttcgg tggtcttgtg aacgcgctgc
     2161 ctaacggtcg tcgtgccggt gaaacttttg cgtctggctt tgctcctgaa aatggcgcag
     2221 acaagcgtgg taccaccgca ctattgaatt ccatgaacaa gattaactac aagaactttg
     2281 ccaacggcat caacttcaat atcaagttag acgcttccag ctatgagtgt gatgacggca
     2341 agagtgcctt gggcagcttg tacaaggtgt tcttcaagcg tgaaggtatg caggttcagg
     2401 ccaatatgct ggatcctaag attctgattg aagctcgtga taaccctgag ctgcatccga
     2461 acttgttgat ccgcgtatcc ggttattccg cgtactttaa tgacctgtct ccgaagatgc
     2521 aggatgaagt catctccagg agctcacatg cggcctgatg cgagcctcat taactcgatt
     2581 aatgtaacgg agccccgcaa ggcttcgtta tatttcgatg ttaagcgcaa tacgctcgat
     2641 gacgggccgg gtattcgcac cgtagtcttt tttaaaggct gcccactgtc gtgtacctgg
     2701 tgccagaatc cggaaagtat caataccggt tacgagctgt cattcaatgc tgaaaagtgc
     2761 atcgaatgcg gtgactgtgt gagtgcttgt ccggaacagg caattgatct gacggtaccg
     2821 ggacatatct tgcgtgacaa gtgcacccaa tgctttaaat gtgtcgatgt gtgcccgtcc
     2881 ggtgcgctgg agcagatagg tcgccccact aatgatcttg aagcgctggc tgaagaagtg
     2941 cttgaagatg ctgatttctt tgagatgtcc ggtggtggcc tcacggtttc cggtggtgaa
     3001 gccctgatgc aaatggacag tgtcggcgaa ttatttcagt tggtgaaaag gaagagagat
     3061 attcataccc tgattcaaac cgcagggttg ttcaacttta gccgttttga aagtctggta
     3121 ttgcctcaca ctaataccat ttatttcgat ctcaagctca tcaacagtga tgatcatttg
     3181 acctattgcg gtgttaaaaa cgaacccatc attgagaact ttaaaaaggt tcaggcattg
     3241 gccttgacca ctgatcttga agtattgcct cgagt
//
LOCUS       DAOQLC010000262         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_15997_length_3254_cov_21.721475, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000262 DAOQLC010000000
VERSION     DAOQLC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15997_length_3254_cov_21.721475"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..812)
                     /locus_tag="PL000_21420"
     CDS             complement(129..812)
                     /locus_tag="PL000_21420"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC91499.1"
                     /translation="MFTPVQISRMFLKSYFTILNRSFDVDGNLPMPNGPKIIAMNHTP
                     GFDPLYLPFLLEDTPHFLLQDGMFRIPVIGWMLKGSGQIPVYRGTDKAKEAKEQACAI
                     LRNGGTIAIFPEGQDVELGKRIPAKTGVVRMALETGAPIIPLGLYTSPQNLTRLNFQW
                     EGTPRSGAWQFSGKSYMRIGTPWNPNAQADIHAQTEELMNRIYTLVAEAEVDSHFERH
                     TLLKPIHQW"
     gene            complement(823..1482)
                     /locus_tag="PL000_21425"
     CDS             complement(823..1482)
                     /locus_tag="PL000_21425"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNC91500.1"
                     /translation="MKKIFTIILVTAVLIAAWAFRVPAVNLLGWFGNREAVVNSIQGL
                     GLWGPFVLIVLLVLQVFLAFIPGQALMVACGYIYGFWGGLLISWIGLFAGGQAAFFLA
                     RHYGRPFAERWIAPDVLSKWDKAAAGQGIVFFTLSLVLPIFPNDAMCYVAGLGNITSH
                     RFTAANSLGRGLACLFMSAVGAFGSQLGWQAWVVIAILVILTCLGWLAFKNLKPEFLN
                     A"
     gene            complement(1619..>3254)
                     /locus_tag="PL000_21430"
     CDS             complement(1619..>3254)
                     /locus_tag="PL000_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91501.1"
                     /translation="VTMRGVILLLSLSAFFFGAYNNIFNQITGTAALRAAFLSIGLGA
                     WYDITLQLLPVENRKSRLPWTISLYTLGLISIILLLSTPNSFIGEQGNQIYVARMGIG
                     LPYILYAVFETAACFGIFKNLFSHRKIGMTAQGKFFLVASIFPAVSVLYGALALALAP
                     PLPRLIQDLLIFSGVFLMGLSVANHQTLVERRTTLQDFPITSLTILVITSIYAVISLR
                     FGLPLQLVGPLAAFVVLTHALYDLVRESLERLRIRKEEMLRKQLHKLKSGQTPEDTLH
                     LRLQEGLDLLCSTLDTSSGFIAIKRGGEFVVTASRLSVEIDARFSSSLVSYEDIVQDQ
                     NDQIPNIIYFAPSFDGQIQIAIVGVGKPNDKLDYSRGDLDLLSEVADQIGTIVSLNAP
                     QVQPTQTEIDESGSTLAAAGMIDAIEINPDQDFIKMVEEGLRHLSDYIALGKSPLADR
                     LGVSAGSHIERGRELQQTIVAAIDLLKPSEKRPPEPLPRVWYNHAVLHDAYVEGVPNR
                     EIMARLYISEGTFNRTRRNAIRGLARLLMEKKTTAK"
BASE COUNT          794 a          802 c          899 g          759 t
ORIGIN      
        1 ttgataatca gtcctcattt tgggctggaa tcttaccgtc aggatgccta tacaaatggc
       61 tgggttttgt gtcgggcggg agacgccgcc gagagcatct ttttacaaaa gttatttcga
      121 ccgactacct accattgatg tatcggttta agcagggtat gcctttcaaa gtggctgtca
      181 acttccgctt ctgcgacgag ggtgtagatg cgattcatca attcttctgt ttgtgcatga
      241 atatcagcct gggcatttgg attccagggt gtgccaatac gcatgtagct ttttcctgag
      301 aattgccatg caccagaccg cggagttccc tcccattgaa aattgaggcg ggtcaaattt
      361 tgtggtgacg tatataagcc cagcgggatg atcggtgcgc cggtttcaag cgccatgcgt
      421 accacgcctg ttttggcagg gatgcgtttc cccaactcaa catcctgtcc ttcaggaaag
      481 atggcgatcg taccgccatt gcggaggatg gcacacgcct gttctttggc ttcctttgct
      541 ttgtcggttc cgcgatatac ggggatttgc cctgaccctt tcaacatcca gccgattact
      601 gggattctga acattccatc ctgcaaaagg aagtgggggg tatcttcgag gagaaagggc
      661 aggtaaagcg gatcgaagcc cggggtatga ttcattgcga tgatcttggg gccgttcggc
      721 atggggagat ttccgtcaac atcaaaactg cgattaagga tggtgaaata ggatttcagg
      781 aacattcggg aaatttgtac gggtgtgaac atgtcatcct ccttatgcgt ttagaaattc
      841 aggtttcagg tttttgaaag ccagccagcc aaggcaggtc aatatgacga ggatggcaat
      901 gacgacccag gcctgccagc ccagttgact tccgaacgcg ccaaccgcgc tcatgaagag
      961 gcaggccaat ccgcgcccga ggctgttggc ggccgtaaaa cggtgggagg tgatatttcc
     1021 aagaccggct acgtagcaca tggcgtcatt tgggaagatc ggcagaacca gtgaaagtgt
     1081 aaaaaaaaca attccctgcc cggctgcggc cttgtcccat ttcgagagga catcgggtgc
     1141 gatccagcgt tcggcaaatg gacgtccata atggcgtgcg aggaagaatg cggcctgccc
     1201 gcctgcgaac agaccgatcc acgagatcaa aagcccgccc cagaaaccgt agatatatcc
     1261 gcaggccacc atgagtgcct gccccgggat gaaggcaagg aaaacctgta aaacaagcaa
     1321 tacgatcaga acgaagggtc cccaaagtcc aagcccctga atggagttga ccaccgcctc
     1381 gcgattgcca aaccagccga gcaaattcac agcgggcacc cgaaatgccc aggcggcgat
     1441 caaaactgct gtaaccaaaa tgatggtgaa tatctttttc atggcgaatt cctttcgtgg
     1501 ctccactttc tcaaatttca actgccaaag ccagagaaga aactgccaaa agacgccaac
     1561 ttaaaaaaat cgtccctgaa ggctcaaacc ttcagggacg atgagtaatt acgggcggct
     1621 atttcgccgt tgtctttttt tccatcaaca gcctcgccag cccgcggatg gcgttgcggc
     1681 gggtgcggtt gaaagtgccc tcggaaatat ataaccgcgc catgatctcg cggttgggaa
     1741 ccccttccac gtaggcatcg tgaagaacgg cgtggttgta ccaaacgcgc ggcaacggtt
     1801 caggcgggcg tttttcagac ggcttcaaca ggtcaatggc tgcgacgatg gtctgctgca
     1861 attccctgcc gcgttcgatg tgtgaccctg cacttactcc cagcctgtcg gcaagcggcg
     1921 attttcccaa agcgatgtaa tctgataaat ggcgcaatcc ttcttcaacc attttgatga
     1981 aatcctggtc agggttgatt tcaattgcgt cgatcattcc tgcggcggca agggtcgatc
     2041 cgctttcatc aatttctgtc tgagtgggtt gaacctgtgg agcgttcaac gacacgattg
     2101 tgccgatctg gtctgcgacc tctgaaagca gatccaaatc tccacgggag taatccagct
     2161 tgtcgttggg tttgccgacc cctacaatgg cgatctggat ttgtccgtcg aatgatgggg
     2221 cgaaataaat tatgtttggg atctgatcgt tttgatcctg gacaatatct tcgtatgaaa
     2281 ccagtgagga tgaaaaacgc gcatcaatct cgaccgagag cctgctcgct gttacgacga
     2341 attcccctcc ccgtttaatg gcgatgaatc cgctggatgt atccagcgtg ctgcataaaa
     2401 ggtcgaggcc ttcctgaagg cgcaggtgca acgtgtcttc gggagtttgt ccgcttttta
     2461 gtttgtgcag ttgtttgcgg agcatctctt ctttgcgaat gcgcagacgt tcaagggatt
     2521 cgcgcacaag gtcgtacagg gcatgtgtca gcaccacgaa tgccgcgagc gggccgacca
     2581 gctgcaaggg gagtccgaag cgcagcgaga tcacagcgta gattgatgtg atgaccagga
     2641 tcgtcaggct tgtgatcggg aaatcctgca aggttgtgcg tctctccacc agggtttgat
     2701 ggtttgcaac agataagccc atgagaaaaa cgccgctaaa gatcaacagg tcttgaatca
     2761 ggcgcggcag cggcggtgcg agggcgaggg ccagtgcgcc gtaaagcaca ctcacggctg
     2821 ggaagatgga cgcaaccaga aaaaattttc cctgagcggt catgccgatc tttctgtgtg
     2881 agaacagatt tttaaaaatg ccgaaacagg cggctgtttc aaaaacggcg tacaaaatat
     2941 atggcaagcc gattcccatg cgggcgacgt aaatttggtt gccctgttcc ccgatgaatg
     3001 aattgggcgt acttagcaac aggatgatcg atatcaggcc gagggtgtaa aggcttattg
     3061 tccacggcag gcgtgacttg cggttttcta cgggtagaag ctgcagggtg atatcgtacc
     3121 aggcacccag accgatgctg aggaatgcgg cacgcagggc ggctgtgcct gtaatttgat
     3181 tgaaaatatt attgtatgca ccaaagaaaa aagcggacaa ggacaggagc agaatcactc
     3241 cccgcatggt cacc
//
LOCUS       DAOQLC010000263         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16066_length_3246_cov_27.457850, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000263 DAOQLC010000000
VERSION     DAOQLC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16066_length_3246_cov_27.457850"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PL000_21435"
     CDS             complement(<1..410)
                     /locus_tag="PL000_21435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC91502.1"
                     /translation="MNTTSLPHIVIIGAGFAGLETARGLAKARVRITLIDKNNHHLFQ
                     PLLYQVAAAGLLPSQIAQPVRTIFRRQKNLNFQMGEVTQINFEEKYVKLNGSVIAYDY
                     LVIAVGGRTNFFGFDAIEKHGFQLKDLETAVNTR"
     gene            complement(605..1291)
                     /locus_tag="PL000_21440"
     CDS             complement(605..1291)
                     /locus_tag="PL000_21440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC91503.1"
                     /translation="MSFTFHRIPVGICNCFLLRGEQTILVDAGAWGGTSAFVRGLKSL
                     SVDPKSIDLILLTHGHWDHITCLSDIREMTGAKVAVHGRDQFMVETGEPPFPAGTTPY
                     GRMMAASAKALLHPHLPKIKVDRVLDDNGMSLAEYGIPGKVIYTPGHSMGHSSILLDS
                     GDALVGDMAMNDWYLRLSPGLPVLADDINLVVQSWKKILPMGIKRVYPAHGMDFPAEV
                     MEQEISKFGS"
     gene            complement(1291..2268)
                     /gene="galE"
                     /locus_tag="PL000_21445"
     CDS             complement(1291..2268)
                     /gene="galE"
                     /locus_tag="PL000_21445"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNC91504.1"
                     /translation="MNIFITGGAGYIGSATAEALIKSGHSVTVFDSLVTGHREAVPQG
                     AKFIHASLDDSHALAEALTAAKYDAIMHFAAFIEAGESMKDPGKFFRNNFANSLQLME
                     TAVQAGVKKFVFSSTAAVYQSSEEPLTEDSPIGPTNVYGHTKLMTEQALEWYRQIHGL
                     HFAILRYFNACGALPGRGEAHQPESHLIPRVLQIALGQAESATIFGTDYPTPDGTCIR
                     DYIHIADLVSAHILALGALESKDKMIYNVGSGNGFSVREVIETARKVTGHAIPTVESP
                     RRPGDSARLVASPKRIMSELGWKPTHTNMQEILSSAWEWHTSHPNGYEK"
     gene            complement(2309..>3246)
                     /locus_tag="PL000_21450"
     CDS             complement(2309..>3246)
                     /locus_tag="PL000_21450"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNC91505.1"
                     /translation="APRPDQTNWTSIMRRMITRQVTLFLVIFSLLLGLSACQPANEIV
                     PTQTVEPPTATISPTATQPSITASPTPNNCLTNPGTLKQDVVQTTKPPQEFIIYLPPC
                     YDSDSEARFPVLYLLHGQTYTQDQWVRLGVPNMADQLIQNGTSIPFIIVFPDDRYWNV
                     DAGSGFGDRFINALIPYVDENYRTLANRANRSIGGLSRGGGWAVQLAFEHPDLFGAIG
                     LHSPAIFKDNAPYLDTILKAMSADIRPNLWIDIGDADMELSRAILFEEILTKNDYLHE
                     FHLYSGDHTENYWGGHVDEYMLWYVKQWSRPSLEQ"
BASE COUNT          728 a          804 c          938 g          776 t
ORIGIN      
        1 ttgcgcgtgt tgaccgcggt ctcaaggtcc ttgagttgga agccgtgctt ctcaatggcg
       61 tcgaagccga agaagttcgt gcgcccgccg accgcgatga cgagataatc atacgcaatg
      121 accgagccgt tgagcttgac atacttttct tcaaagttga tctgtgtcac ttcgcccatt
      181 tggaagttga gatttttctg cctgcgaaag atcgtgcgca caggctgcgc gatctgcgaa
      241 ggcagcaagc ccgccgccgc aacctgatac aacaacggtt gaaagagatg atggttattc
      301 ttatcaatga gcgtgatgcg cacgcgcgct tttgcaagtc cgcgtgcggt ttcaagccct
      361 gcgaaccctg cgccgataat gacaatgtgg gggagtgatg ttgtgttcat ggttcctcta
      421 atgtgattta tttcacaatt attataacca aaatggtctt atttgggaat gttttcacaa
      481 tgggagattg gtactggagt gcggacttca gtccgcaatt tataggggcg gactgaagtc
      541 cgcactccga aaataaaaaa agccctcccg cttctttcta cgggagggct gattctggct
      601 tcgatcagga tccaaacttt gagatttcct gctccatcac ctcggcagga aaatccatgc
      661 catgagcggg atacacgcgt ttgattccca ttggcaaaat tttcttccag ctttgcacca
      721 caagattgat gtcatctgcc agcacaggca gacctggcga aaggcgcaaa taccaatcgt
      781 tcatcgccat gtccccaacg agagcatcgc ccgaatcaag caggatgctc gaatgtccca
      841 tcgaatgacc tggcgtgtaa atcaccttgc ctggaatgcc gtactccgcc agtgacatgc
      901 cgttgtcatc cagcaccctg tccactttga tctttggcag atgcggatga agcagcgcct
      961 tggctgatgc cgccatcatt cgcccatacg gagttgtgcc agcagggaag ggcggctcgc
     1021 ctgtctcgac catgaactgg tcccgtccat gaacagcgac cttcgcccca gtcatctcgc
     1081 ggatatcgct caggcaggtg atgtgatccc agtgtccgtg tgtgagcagg atcaggtcaa
     1141 tggacttcgg gtccacgctg agggatttca gcccgcgcac aaaagcggat gtgccgcccc
     1201 atgcgcccgc gtcaacgaga atcgtctgct cgccgcgaag cagaaaacaa ttgcaaattc
     1261 caacagggat gcggtgaaaa gtaaaagaca ttatttttcg taaccgttcg gatgcgatgt
     1321 gtgccactcc cacgcgctgg agagaatctc ctgcatgttg gtgtgggtgg gcttccagcc
     1381 aagctcgctc atgatgcgtt tgggcgaagc gaccaatcgc gctgagtcgc cggggcggcg
     1441 cggagattca actgtcggga tggcgtgccc cgtcactttg cgggcggtct cgatcacctc
     1501 acggacagag aatccatttc cgctgccgac gttgtagatc atcttgtctt tggattcgag
     1561 cgcacccaaa gcaaggatgt gcgccgaaac caggtccgcg atgtggatgt aatcacggat
     1621 gcaggtgccg tcgggggtgg ggtagtcggt gccgaagatg gtggcagact cagcctgacc
     1681 cagcgcgatc tgcaacacgc gcgggatgag atgtgactcg ggctggtgcg cttcaccgcg
     1741 cccgggcaat gctccgcagg cgttgaagta acgcaggatg gcaaagtgca atccgtggat
     1801 ctgacgatac cattcgagcg cctgctcggt catcaacttg gtgtgaccat agacattggt
     1861 cggtccgatg ggggagtctt cggtcaacgg ctcttcgctg gattgataca ctgcagcagt
     1921 ggaggaaaat acaaattttt tcacacccgc ctgcacggca gtttccatca gttgcaggga
     1981 gttggcaaaa ttattgcgga agaacttgcc cgggtctttc atgctttcgc ccgcttcgat
     2041 gaacgcggca aagtgcatga tggcatcata tttggcggcg gttagcgctt cggcgagtgc
     2101 atgactgtcg tccaggcttg cgtggatgaa ttttgcgccc tgcggcacgg cttcacgatg
     2161 gccggtgacc aatgagtcaa ataccgtgac ggaatgacct gatttgataa gtgcctcggc
     2221 ggtggctgag ccgatgtagc ctgcgccgcc agtaatgaag atgttcatta attctcctga
     2281 attcaaattt gatcaaaaaa attatatcct attgttcgag cgatggtctg gaccactgct
     2341 ttacatacca gagcatgtat tcatccacat gccctcccca gtaattttcg gtgtgatcgc
     2401 cggagtagag atggaactcg tggaggtagt cgtttttagt gagtatttct tcaaacagga
     2461 tggcgcgcga tagttccatg tcggcatcgc caatatcgat ccacaggttg gggcggatgt
     2521 ccgcggacat ggctttgaga atggtatcca gatagggggc attgtctttg aagatcgccg
     2581 gtgagtgcaa tccgatcgct ccgaataggt caggatgttc aaatgcgagt tgcacagccc
     2641 agcctccgcc gcgcgaaagc ccaccgatgg aacggttggc acggtttgcc agggtgcggt
     2701 aattttcatc cacgtatggg atgagcgcat tgatgaaacg gtcgccgaaa cctgagcctg
     2761 catccacatt ccagtaacgg tcatcgggga aaacgataat gaatggaatg gaggtcccat
     2821 tttgaatgag ctggtcggcc atgttgggga cgcccagtcg cacccattga tcttgagtgt
     2881 aggtctgccc gtggagcaaa tagagcacag gaaagcgggc ttcgctgtca ctgtcataac
     2941 atgggggcag gtaaatgata aattcctgcg gcggttttgt cgtttgaaca acatcctgct
     3001 ttagagtgcc aggattggtt aaacagttat tcggggtggg ggaggctgtg atcgacggct
     3061 gggttgctgt gggtgagatg gtggcggttg gcggttccac tgtttgggtg gggacaatct
     3121 catttgctgg ctggcaggct gaaagtccga gtagaagcga aaaaataacc aaaaaaagag
     3181 ttacttgacg ggtaatcatc cttcgcatta tactcgtcca gttcgtttga tcggggcgtg
     3241 gcgcag
//
LOCUS       DAOQLC010000264         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16078_length_3245_cov_11.789028, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000264 DAOQLC010000000
VERSION     DAOQLC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16078_length_3245_cov_11.789028"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1681)
                     /locus_tag="PL000_21455"
     CDS             complement(95..1681)
                     /locus_tag="PL000_21455"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC91506.1"
                     /translation="MKTRKNRWMTALILTLMLALTIVTPALADDGGPTAEQVADLTLG
                     INILWMILGGFLVFFMQAGFALVETGFTRNKNVVHTMMMNMMVFCIGAVGYWLVGFAF
                     QFGGVNAAYPEVARAGVVAGEWAHSPITLGDWTGFLSAPLVRFGQFGFLGGSGFLLAG
                     FGANAGLLAFFLFQMVFMDTAATIPTGSMAERLKFSGFIWMSLWVSMVVYPIIGGWIW
                     GGGWLQNIGRSLGLGNGAVDFAGSGPVHMIGGAIALAGAIVIGPRIGKFNKDGSANSI
                     PGHSISLGILGTIILFFGWFGFNPGSSLGFVGGFRNLAVIAAINTLMAGAAGGISAMT
                     YAWLLGPSKKPDAGLSVNGILAGLVAITAPCAFVDSWAAVVIGLIAGLIACWATFQLE
                     KWKIDDPVGAVPVHFFNGAWGVLAVGIFANGNPDTAAWNGVESPVTGLLYGGNTQILA
                     QAAEIVSIFAFAFGLSYVFFKILAAFKVLRVSREVELQGLDIPEMGSIGYPVDWEPNL
                     DTSEPSVGAYPKSALGSPAD"
     gene            complement(1749..3053)
                     /locus_tag="PL000_21460"
     CDS             complement(1749..3053)
                     /locus_tag="PL000_21460"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNC91507.1"
                     /translation="MFFTLIVFVLAYIVIASEKFPRHWVSLIGGGLLILFGILSPYEA
                     FNYINWETLGLLAGMFLLVSILQEAGFFSWLAMRALKKVNYHPAALFVTLIVFATAMS
                     MFMDSITVMLFLSALTLQLCRLLKLDPVPLIIAEVCAANTGGSATLVGDPPNVILGTT
                     LGFNFADFIMHTGPISIMAALLLLGMFYLTNRQSLKNAHSLLTDETINEIEALHNEPL
                     HAHMTRIGLSFFSLAVILLVFHTPLSHITGFHISAAMAAILPAALALVFLRNDDRKKA
                     MLKVDMESILFFAGLFVLIGGLEKINIFESLAKGLASFGNEDHGSLVMLLHWIPGLAS
                     GIVDNVPLALAMSYVLKDLAAIPGMPALGLMVWSLALGVDIGGNLTPIGASANVVAYS
                     FVEKNHGSIGWKRWLMVAVPPTMLIMVFASLSVLLKGRIGWF"
BASE COUNT          828 a          837 c          897 g          683 t
ORIGIN      
        1 acccaaacgg gtacttaatg ggaaagtgga cggggaagcg gcccgtccac ttttggcacc
       61 ttacggtaac cagaagagaa gaatagtcac acctttaatc tgcaggagaa ccgagtgcgc
      121 tttttggata ggcgccaact gaaggttcgg aggtgtctaa attgggttcc caatccaccg
      181 ggtaaccgat ggatcccatt tcggggatat ccagcccttg taattccact tcgcgtgaga
      241 cgcgcaggac tttgaatgca gctagaattt tgaaaaagac atacgaaaga ccgaaagcaa
      301 atgcaaagat gctaacgatt tcggccgctt gtgcgaggat ctgggtgttt ccaccataga
      361 gcagacctgt taccggcgat tccactccgt tccaggcagc ggtatccggg ttgccattgg
      421 cgaagatgcc aacagccagc acaccccagg cgccgttgaa gaagtgaacc ggcaccgcgc
      481 caacagggtc atcgatcttc catttttcga gttggaaggt ggcccaacat gcgatcaacc
      541 cggcgatcaa tccgatgacg accgccgccc acgagtcgac gaaggcgcag ggggctgtga
      601 tggcaaccag accagccaaa attccgttga cggaaaggcc ggcatctggc tttttgctcg
      661 gtccgagcag ccaggcatag gtcatggctg agatgccgcc tgccgcacct gccatcagag
      721 tattgattgc ggcaatcacg gccaggttgc ggaatccgcc gacaaagccg agagaggagc
      781 cagggttgaa gccgaaccag ccgaagaaca ggatgatggt gccaagaatt cccagtgaaa
      841 tgctgtgtcc gggaatgcta ttggcggaac catccttgtt gaattttccg atgcgcggac
      901 cgatcacgat ggctcccgcc aaggcaatgg caccgccgat catgtgaaca ggacccgacc
      961 cggcaaagtc tacggctccg ttgccaagcc cgaggctgcg gccaatgttt tgcagccagc
     1021 cgccgcccca gatccatccg ccgatgatgg ggtacaccac catgcttacc cacaggctca
     1081 tccatataaa gccgctgaac ttgaggcgtt ctgccatcga accagtggga atggtggctg
     1141 ccgtatccat gaaaaccatt tggaaaagga agaaagcaag cagccctgca ttggctccaa
     1201 aaccagcgag caagaacccg cttccaccga ggaagccaaa ttgcccaaag cggaccaatg
     1261 gcgccgagag gaagcctgtc caatcgccca atgtgatggg agagtgtgcc cactctccag
     1321 caacaacacc agcacgtgcc acttcagggt aggcggcatt gaccccgccg aactggaagg
     1381 caaaaccaac cagccagtac ccgactgcgc caatgcagaa caccatcata ttcatcatca
     1441 tggtgtggac tacgttcttg ttgcgggtga agccagtttc cacaagagca aagcctgcct
     1501 gcataaagaa taccaggaag ccgccgagga tcatccacag aatgttgatt cccaatgtca
     1561 ggtctgccac ctgttcggca gttggtccgc cgtcgtcagc aagggcgggt gtgacgatgg
     1621 tcagtgccag catcagggtg aggatgagcg cggtcatcca gcgatttttt ctggttttca
     1681 ttttctcttc tcctttttca gatcatccac cgtcttttgg cgtttgtgag acggttgaaa
     1741 attttgttct agaaccagcc gatccgtcct ttgagcagga cgctcagcga ggcaaatacc
     1801 atgatgagca tcgtaggcgg aactgcaacc atgagccatc gcttccaacc gatggaaccg
     1861 tgatttttct caacaaatga gtaggcaacg acgtttgccg atgcgccaat gggggtgagg
     1921 ttgccgccga tatccacacc tagagccagc gaccagacca tcagcccaag ggcgggcatg
     1981 cctgggatcg cggcaaggtc tttgagcacg taactcatgg caagggcaag cgggacgtta
     2041 tccacaattc cgctggcgag acctggaatc cagtgcagga gcatgaccag gctgccgtgg
     2101 tcttcatttc caaagctggc aagtcctttt gccagtgatt cgaaaatgtt gatcttctcc
     2161 agcccgccga tgaggacgaa caagcccgcg aaaaagagta tgctttccat atccactttc
     2221 aacattgctt ttttgcggtc atcattgcgc aggaatacaa gagccaatgc cgcaggcaga
     2281 attgctgcca ttgctgcgct gatgtggaag ccagtgatgt ggctgagcgg cgtgtggaat
     2341 accaacagga tgaccgccag tgagaagaag gacagcccga ttcgtgtcat gtgcgcatga
     2401 aggggttcgt tgtgcagggc ttcgatctca ttgatggttt catcggtcaa aaggctgtgg
     2461 gcattcttca acgattgacg gttggtcagg tagaacatgc cgagcagcaa aagggccgcc
     2521 atgatcgaga tgggccccgt gtgcatgatg aaatcggcga agttgaatcc gagtgtggtt
     2581 ccgaggatca cattgggcgg gtcaccgacg agggtggcag atccgcctgt gttggcggcg
     2641 catacttcag cgatgatcaa aggaacgggg tcaagtttca gcagtcggca gagttgaagc
     2701 gtgagcgcgg agagaaataa catcacggtg atgctatcca tgaacatgga catggctgtg
     2761 gcgaagacga tcagagtcac gaacagggct gcggggtgat aattcacttt ttttagagcc
     2821 cgcatggcaa gccaggaaaa gaagcctgct tcctgcaaaa tggaaaccag caggaacatg
     2881 cctgcgagta acccaagcgt ctcccagttg atgtaattga aggcttcata cgggctgaga
     2941 attccgaaca ggatcagcaa tccgccgccg atcaacgata cccaatggcg ggggaatttt
     3001 tcactggcga tcacaatata tgcaagcaca aagacgataa gtgtgaaaaa catggagcac
     3061 ctcaattcct gaacggtgaa gctgtgtact tgcgttttat tgtatggaat taaggtctcg
     3121 cgtctattac cctaatggag aatattgttt catcatttcg catgccttat tacccaaatg
     3181 gatcatgttt tcatgatgtc gaactttgat aataaaaaac gcctacccat ttgggtaagc
     3241 gtttt
//
LOCUS       DAOQLC010000265         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16133_length_3239_cov_19.574435, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000265 DAOQLC010000000
VERSION     DAOQLC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16133_length_3239_cov_19.574435"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1354)
                     /gene="gguB"
                     /locus_tag="PL000_21465"
     CDS             complement(191..1354)
                     /gene="gguB"
                     /locus_tag="PL000_21465"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC91508.1"
                     /translation="MKTLSNLFKNNIREYGMLIALIVIMIFFYFMTDGILMKPINITN
                     LVLQNSYVIIMALGMLLIIVSGWIDLSVGSVAAFIGSIAGVMIVKWEWNWILVMGICL
                     TIGGVIGAWQGYWVAYLKIPAFIVTLAGMLIFRGLTLVMVRGEAIGPFPEGFRNISGG
                     YIPDFFHYEGFHLTTIVVGVLVSILLIFSDIRTRRNQQKYGFEVTPTYFFIVKQILIT
                     AAVMGFCYLMASYKGIPNLLVIMFPLVTIYSFLTSQTVIGRRIYAMGGNEKAARLSGV
                     NTQRLLFLTFVNMGVLAALAGMVVASRLNSSTPNAGEGFELDVIAACFIGGASASGGI
                     GTVIGAVVGAFLVGVLNNGMSILGVGIDWQKVVKGMVLLLAVLFDVYNKNKSS"
     gene            complement(1351..2892)
                     /gene="gguA"
                     /locus_tag="PL000_21470"
     CDS             complement(1351..2892)
                     /gene="gguA"
                     /locus_tag="PL000_21470"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNC91509.1"
                     /translation="MSDVILEMRNITKTFPGVKALDNVSFSVKQGEIHALVGENGAGK
                     STLMKVLSGVYPYGSYDGEIFFEGKECRFHDITQSEDVGLVIIHQELALIPYLSIAEN
                     LFLGNERANFGIIDWNESILRTKELLSKVGLHESPDTLITNIGVGKQQLVEIAKALAK
                     KVKLLILDEPTASLNENDSEKLLELLLQFKENGIASILISHKLSEVSKVADSITILRD
                     GATIETLDCHKDTITEDRIIRGMVGRDITHRFPPRESRIGEVVFEIKDWNVYHPQHED
                     RKVSNNVNIKVHRGEVVGISGLMGAGRTELAMSVFGRAYGTRISGKAFKDGIEIDIST
                     IDKAIANGIAYATEDRKAYGLVLIEEIKDNITLSNLNAISDAGVINEPKELTVTREYR
                     DKLNIKCSSILQLALNLSGGNQQKVVLSKWLFANPEILILDEPTRGIDVGAKYEIYTI
                     INQLASEGKAIILISSELPEILGVCDRIYVMREGKIVGEMPAKEASQELVMKHIMFEQ
                     EAIAQ"
BASE COUNT          873 a          747 c          701 g          918 t
ORIGIN      
        1 ttgcaataaa gtgtttagta tgtcttccat ctgcctgtcc tttgggaacg caatacctac
       61 gaaaaccaaa ttcgctttaa gtcaatcttt taaatatagt ttttaatatt tacagaggca
      121 atactgatga gatctttgtt gttgagaacg agagcagtcg ttgaaactgc tctcgttggt
      181 ggaccaatta ttacgaactc ttgtttttgt tgtacacatc aaacaaaacg gctaaaagaa
      241 gtaccatgcc ttttacgacc ttttgccagt caatacctac gccaagaatg gacatgccat
      301 tgttcaacac accgacaaga aaggctccaa ctactgcacc aataaccgta ccaataccgc
      361 cactcgcaga agcaccaccg atgaagcacg cggcgattac gtcaagctca aagccttcac
      421 ctgcatttgg tgtagaactg ttcaagcggg aggcgacaac catccccgcc aatgctgcaa
      481 gaacgcccat gtttacaaag gttaggaaca aaaggcgctg tgtgttgaca ccggaaagac
      541 gggctgcctt ctcatttcca cccatggcat agatacgacg cccaatcaca gtttgactgg
      601 tgaggaagga gtaaatcgtc accagcggga acatgattac aagcaagttg gggatgcctt
      661 tataagaagc catcaagtag caaaagccca tcactgcagc tgtaatcaag atctgtttca
      721 ctatgaagaa gtatgtggga gtgacttcaa atccgtattt ttgctgattg cgtcgcgttc
      781 gtatgtcaga aaagatcaag aggatggata ctagaacgcc tactacaatg gttgtaaggt
      841 gaaacccttc atagtggaaa aaatctggaa tatagccgcc actaatgttg cggaagcctt
      901 ccgggaacgg tccaatcgct tcgccgcgaa ccatgacaag ggtaaggccg cggaagatca
      961 acatgccagc cagcgtcacg atgaatgcag gaattttcag atacgccacc caatatccct
     1021 gccaggcgcc aattacaccg ccgatcgtaa gacaaatccc cattaccaat atccaattcc
     1081 attcccattt tacgatcatt acaccggcta tggagccgat gaaggcagct actgaaccga
     1141 ccgagagatc gatccatcct gaaacaatga ttaacagcat gcccagtgcc atgatgatca
     1201 cgtagctgtt ttggaggacg agattggtga tgttgatcgg tttcatcaga atgccatctg
     1261 tcatgaaata gaagaagatc atgatcacga tcaacgcgat caacatgccg tactcgcgga
     1321 tattgttctt gaaaaggttg gacaaagttt tcattgtgcg atagcctctt gttcaaacat
     1381 gatgtgcttc attacaagtt cttgcgacgc ttccttggcg ggcatttcac cgacgatctt
     1441 gccttcgcgc ataacataaa ttcggtcaca aacccctaat atttcgggta actcggagga
     1501 gattaaaata atcgcctttc cttcactggc aagttgattg atgatcgtgt aaatttcgta
     1561 tttggcgccc acgtcaatgc cgcgggtggg ctcatccaaa ataagaattt cagggttggc
     1621 aaaaagccac ttgcttagta ctactttttg ttgattgccg ccagagaggt tcagcgccaa
     1681 ttgaaggata ctggaacatt taatgtttaa tttatctcga tattcgcggg ttacggtcaa
     1741 ttcctttggc tcgttaatga caccagcatc tgagattgcg ttgaggttgg ataatgtgat
     1801 gttgtctttg atctcttcga taagtaccaa gccataggct tttctgtctt ccgtcgcata
     1861 tgcaattcca tttgcaatgg ctttgtcaat tgtgctaatg tcaatttcta ttccgtcttt
     1921 gaaggccttg ccgctaattc tggtgccgta ggcgcgccca aaaacgctca tcgcgagctc
     1981 tgttctgcca gctcccatca agccagatat tccaaccact tcaccacgat gaacttttat
     2041 gttcacattg ttgctgacct ttcggtcttc gtgttgtggg tgatatacat tccagtcttt
     2101 gatctcaaat acgacttcac cgattcttga ttcacgcggc ggaaaccggt gtgtgatatc
     2161 tcgaccgacc atgccgcgga taatgcgatc ttctgtgatg gtgtctttat gacaatccaa
     2221 tgtctcgatt gtggctccat ctcgcaggat tgtgatcgag tcggccactt tggatacttc
     2281 gctaagttta tgagatatca aaatagaagc aataccattc tctttgaatt gaaggagaag
     2341 ttcaaggagt ttctcgctgt cgttttcgtt gagagaggcg gtaggctcgt ctagaataag
     2401 aagtttgact tttttggcca gggctttggc aatttccacc aattgctgtt tgcctacacc
     2461 aatgtttgta attaatgtgt caggcgactc atgtaacccc actttgctca ataactcttt
     2521 tgttcttaag atcgactcat tccaatcgat aattccaaag tttgcacgct cgttgccaag
     2581 gaacaaattc tcagcaatgg aaaggtaggg aattaatgca agttcttgat gaataatcac
     2641 caatccaaca tcttccgact gggtaatatc atggaatctg cattccttgc cttcaaaaaa
     2701 tatctctcca tcataggaac cgtatggata gactccgctt agaaccttca tcagggtcga
     2761 ttttccagcc ccgttttccc caaccaaggc atggatttct ccctgcttga cagaaaaact
     2821 tacattatca agtgccttaa caccagggaa tgtctttgta atgttacgca tttccaaaat
     2881 gacatcagac atggttttct cgtccttgat ctatttaatg ataatgaaac gatattatgt
     2941 ggtaattcta attcaaaaat ccacaatttt ttcaatgagg ttggcttgtc ggagcttata
     3001 gcaagcctat ggtcttgttg acccctgtac gaggacggga tgccctcgta caggggtgtg
     3061 gaacttttac ttcagatctt caggtttgat gtaaccactg gcaatcagca gttcttcgta
     3121 gttagtgata tctacgctgt agggggtcaa cagataggag ggaacgacct tgacgccatt
     3181 gtcataggtc ttggtgtcgt tgatcggtac ttccttgccc tgcaaagcgg catccacca
//
LOCUS       DAOQLC010000266         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16134_length_3239_cov_16.132852, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000266 DAOQLC010000000
VERSION     DAOQLC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16134_length_3239_cov_16.132852"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..3064)
                     /locus_tag="PL000_21475"
     CDS             complement(5..3064)
                     /locus_tag="PL000_21475"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNC91510.1"
                     /translation="MRPDDSHRLTILSAEEIDDLYSVPQFTEEDRQLFFDFSPPEIAA
                     LGTHKTSVAAYLALELGYFKAKQQFFTFEQNEVAADLRYILHRYFHGKNIAWIKPPSR
                     PRRILSQQTILQLFNFRQCDKALRSELEQKARRVATLSTHPLFIMRELLQYLGSERIV
                     VPSYSSMQDLVGRVVTYERNRITQLLNRALTPETESQIDALLQADEQIYTIQVLKREP
                     KDFSYKELRQEVERRKFFQPLHEFAKAFLDTVGISNESSKYYASLVNFYTVYKLRRMA
                     PGTTRLYLLCFAYDRFRQINDNLVDAFIHLVDHYEQQAKEAAAQAMQQALSDAAENLQ
                     AAGRVLNLFVDNTIPGTTPFSAVKEKAFSLLELGRFTAVTDYLQNIAFDKTGFEWSYY
                     TALSHKFKLNLRQLFVGLDFAGRIEDAPLLDAVLFLQDLQRHNKSPRQADSTQFPVKL
                     IPKHLQRYLFSGDKEKRLEVDRYEFLVYRLLRNALEAGDVYVRNSNEFRRFEDDLISD
                     ERWQDKETVLREVGMPILLAPIADTLTTFRDELERKIEIVNQRIADEENRHIKVKGKG
                     EKRRWSLIYPNTDEPINSPFYGRIPGIGIADLLWFVAGKTGFLQAFTHVLDRYVKQEP
                     DPREVLACIVAMGTNMGLGKMAEVSGLGFASLLSTARNFLRLETLREANDAISNAIAT
                     LPAFHLFDIQDEIHSSSDGQRLETQIDTFNARHSPKYFGLDKGVSACTVVANHVPLNT
                     KVIGAHEHESHYVFDLLYNNTSDIRPDRHSTDTHGTNQVNFWILYAFGYQFAPRYRDL
                     YKRIGSLVGFQHPSHYGDLLIKPARKVNGELIIQEWPNIQRIMASLAQKDVTQATIIR
                     KLSSYARQNQTKKALWELDALCRTLYILDFVDDVTLRQSVQKALNRGEAYHRFRRAVA
                     YVNGGKFRVKTEAEQQIWNECSRLIANAIIYYNTLLLSRVYEQKLATSDQEAIAIVKG
                     ISPIAWQHINLYGTFEFSTAPPKVDIDAVVAYYADPQRWSNAINELD"
BASE COUNT          743 a          828 c          845 g          823 t
ORIGIN      
        1 gcacttaatc taattcatta attgcgttgc tccaccgttg agggtcggcg taatacgcta
       61 ccacggcgtc aatatccact tttggcggtg cggtgctaaa ttcgaacgtg ccataaagat
      121 tgatatgctg ccatgcaatg ggagaaatcc ctttaacaat ggcaatagcc tcctgatcgg
      181 aagttgctag tttttgttca tacactcgcg ataacaacaa agtgttgtag taaatgatgg
      241 cgttggcaat caggcgggag cactcgttcc agatctgctg ctcagcctcg gtcttaaccc
      301 ggaattttcc accgttaacg tacgccaccg cacggcggaa tcggtgataa gcctcacctc
      361 gattcagcgc cttttgtaca ctctgtcgta gcgtcacgtc gtcgacaaaa tcgagaatgt
      421 agagggtccg acacagggca tctaactccc acaatgcctt cttcgtttga ttctggcggg
      481 catagctgct cagcttccgg ataatggtcg cctgcgtaac gtccttctgc gcaagcgacg
      541 ccatgatccg ttgtatattc ggccattcct gaatgatgag ctccccgttc accttccgtg
      601 cgggcttaat cagcaggtcg ccatagtgac tcggatgctg gaatccgacc agggagccga
      661 ttctcttgta caaatcccga taacggggag caaactggta accgaatgcg tacaagatcc
      721 agaaattgac ctggttggtg ccatgtgtat cggtcgagtg acggtcaggc ctgatatcgg
      781 aggtgttgtt gtacagcaaa tcgaagacgt aatggctctc atgctcgtgc gcgccgatga
      841 ctttagtatt gagcggtaca tggttggcta ccaccgtgca ggcgctgacc cctttgtcca
      901 atccaaaata cttgggggag tgtcgagcgt tgaaggtatc gatttgtgtc tccagccgct
      961 gcccatcact acttgagtgg atttcgtctt gaatgtcgaa tagatgaaac gccggcaaag
     1021 tggcgatcgc gttgctgatt gcatcatttg cttcccggag ggtttccagg cgtaagaaat
     1081 tgcgggcagt tgatagaagc gatgcaaaac ccaagccaga tacttccgcc atttttccaa
     1141 gacccatatt ggtgcccatg gcgacgatgc aggcgagaac ttcccgcgga tctggctcct
     1201 gtttcacgta ccgatcgagt acatgggtga atgcctgcaa aaatcctgtc ttgccagcga
     1261 caaaccagag cagatcggca atgcctatgc ctggaattcg tccatagaat gggctattaa
     1321 tcggttcgtc cgtgttgggg taaatcaatg accagcgacg cttctctcct ttccctttta
     1381 ccttgatgtg tcgattctct tcatcggcga tgcgttgatt cacaatctcg attttgcgtt
     1441 ccagctcgtc gcgaaatgtt gtcaaggtat ccgcaatggg agcgagcaat atgggcatgc
     1501 caacttcacg taggacggtc tctttgtcct gccatcgctc gtcgctgatg agatcatctt
     1561 cgaagcgccg gaactcgtta ctgttgcgga catagacgtc gccagcctcc aaggcatttc
     1621 gcaggaggcg ataaaccaga aactcgtagc gatcgacttc cagtcgcttc tccttgtctc
     1681 cggagaacaa atagcgctgc aggtgttttg ggatcagctt caccggaaat tgcgtggaat
     1741 ccgcttgtcg tggcgatttg ttatggcgct gcagatcttg cagaaatagc acggcatcca
     1801 gtaacggtgc atcctcaatg cggccagcaa aatccagacc gacaaataac tgtcggagat
     1861 tgagcttaaa tttgtgcgat aaggcggtgt aatacgacca ctcgaaacca gtcttgtcga
     1921 acgcgatgtt ttgcaggtag tccgtcaccg ctgtaaaacg cccaagctcc agcaacgaaa
     1981 acgctttttc cttaacggcg gagaatggtg tagtcccagg gatggtgttg tcgacgaaca
     2041 agttgagcac tcgcccggcg gcttggagat tttctgcagc atcgctgagc gcttgctgca
     2101 ttgcttgcgc cgcagcctcc ttggcctgtt gctcgtaatg atcgaccaga tgaatgaatg
     2161 cgtcgaccag attatcattg atctggcgaa atcgatcata agcgaaacac agcaaataga
     2221 gccgtgttgt ccctggcgcc atacgccgta gcttatagac ggtatagaaa ttgaccaacg
     2281 atgcgtaata tttgctgctt tcgttggaga tgccaaccgt gtctagaaat gctttggcaa
     2341 actcgtgcag gggctgaaaa aacttccgcc gctccacttc ctgccgcagc tccttgtagc
     2401 tgaagtcctt cggctcgcgc ttcagtacct ggatcgtgta tatctgttca tcggcttgta
     2461 acaaggcatc tatttgtgat tcagtctctg gtgtcagcgc ccgattgagt aattgggtga
     2521 tgcggttgcg ctcgtaggta acgacgcgcc caaccaggtc ctgcattgac gagtaggacg
     2581 gtacgacgat ccgttcactg cccagatact gcaatagctc acgcatgata aacagcggat
     2641 gggtcgacag tgtcgcaacc cgtcgcgctt tctgttccag ttcagatctg agcgctttat
     2701 cgcattggcg aaagttgaac agttggagaa tggtctgctg cgacaggatt cggcgtggcc
     2761 gcgatggtgg tttgatccac gcgatatttt taccgtggaa atagcgatgc aagatgtagc
     2821 gcaagtcagc agccacttca ttttgctcga atgtgaaaaa ctgctgcttg gctttgaaat
     2881 atcccaattc cagagccaga taggctgcga cagaggtttt gtgcgtgccc agcgcagcta
     2941 tctctggcgg actgaaatca aaaaacagtt gtcgatcttc ttcggtaaat tgcggcacac
     3001 tatataaatc gtcgatttcc tccgcagaca ggatcgtgag gcggtgagaa tcgtcagggc
     3061 gcatcactat tcctgttggc tgcttggacg gctggcggat agccgcctgg tttcaatgcc
     3121 atattgggaa aagggatcca ccccatcgat atagcgcatc gccgacttca catccttcca
     3181 gccaacgtat tccatcagcg tcttcagatc ccagccgttg gccgtggccc atccggcaa
//
LOCUS       DAOQLC010000267         3217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16326_length_3217_cov_24.435484, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000267 DAOQLC010000000
VERSION     DAOQLC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16326_length_3217_cov_24.435484"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..606
                     /locus_tag="PL000_21480"
     CDS             46..606
                     /locus_tag="PL000_21480"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HNC91511.1"
                     /translation="MQTLLKLSQRIDAITEYFGKASAFCVLLTIVIGFYNVVARYLGR
                     FIGFKLTTNALIELQWYLFSLTFLLGFSYILKHGENVRVDFLYTNWSPKTKAWVDLVG
                     HALFLVVFCIIGLYVTTHPVLQSWGRLPDGTFGTWELSPDPDGLPRAPIKSMILVGFG
                     MLLFQTISEIIKNIAIIQRPQTTDSK"
     gene            653..1999
                     /locus_tag="PL000_21485"
     CDS             653..1999
                     /locus_tag="PL000_21485"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNC91512.1"
                     /translation="MTHFEWLAIVMFVGFFIFIMSGYPVGYSFAATAILFGGIGYALG
                     AFDLNLLKLLPSRWFGTMSDFTLLAIPFFIFMGAVFERSGLAEELLETVGRLMGRVRG
                     GLALAVILVGTLLAATTGVVAATIIMMGMLALPTMLRHGYDKQLATGVITASGTLAQM
                     IPPSLVLVVLSDQIGVPVGDLFLGAVIPGLLLSSLYALYVIIITRIKPDLAPALPPEA
                     FSVTGADLLRKSVRVIIPPGLLVFSVLGSIFFGLASPTEAGSVGAVGACLLALMNKRL
                     TTKLIQDAAMSTARLTALVITILFSSTFFSLVFDGLGGQRLITDLLVNLPGGFWGFII
                     VANIAIFLLGVFLEFVEICFIAMPLFVPAAHALGIDMAWLAIVFAINLQTAFISPPVG
                     FSLFYLQTTAPKEVPTSDIHKGALPFMALQVLALIIVIIFPQTVTWLIELSAKSLP"
     gene            complement(2068..3171)
                     /gene="dctP"
                     /locus_tag="PL000_21490"
     CDS             complement(2068..3171)
                     /gene="dctP"
                     /locus_tag="PL000_21490"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HNC91513.1"
                     /translation="MNRRDFLKVAGMGVAASALAACAEPAPAAGNAAAEDLPVLDWQM
                     ATSWSTGLDILFGATQKFAERVKALTGGKFTITPRAGGELAKATEVLDVVSSGAVPIG
                     HTASYYYIGKSWVLAFGTSLPFGLTASQQNAWLYNGGGLELLQDFYAKKFNVIQFPAG
                     NTSAQMGGWFRKEINTLADLQGLKMRIPGLGGQVMSALGVQVQNIPGGEIFQALETGA
                     IDAAEWVGPYDDEKLGLHKATGFYYFPGWWEPGPSLELQINLDEWNKLPALYQEAIKA
                     ASYEANVAMAAHYDAVNGEALERLLAGGVKLMSYSDEIMQAAQEKAFELYDAKSAEDA
                     EFKAVYEKWMEFRTRIQKWSFTNEFALLKFMAG"
BASE COUNT          677 a          968 c          726 g          846 t
ORIGIN      
        1 agaatttttt atgctatgat tcggcatctt cgccaaagga tagccatgca aacactcctc
       61 aaactctcgc aacggataga cgccatcacc gagtatttcg gcaaagcctc cgccttctgt
      121 gttctgctga ccatcgtcat cggcttctac aacgtggttg cgcgctacct cggcagattc
      181 attggcttca aactgaccac caacgcactc atcgaactgc agtggtattt attctcgctc
      241 acattcctgc tgggtttttc ttacatcctc aaacacggag aaaatgtgcg cgtggatttt
      301 ctctacacaa actggtcgcc aaaaacaaaa gcctgggtgg atctggtcgg ccatgcgctt
      361 ttccttgtcg ttttttgtat cattggctta tatgtcacca cccatccagt tctgcaatcc
      421 tgggggcgcc tgcccgatgg cacgttcggc acctgggaac tctcccccga ccccgatggc
      481 ctgccgcgtg cgcccatcaa aagcatgatc ctcgttggtt ttggcatgct gctgtttcaa
      541 acaatttcag agatcatcaa aaatattgcg atcattcaaa ggccgcaaac tacagattca
      601 aagtagacct tcaacctgca acttgcacct gtaaccctgg accctcacac ccatgaccca
      661 ttttgaatgg cttgccatcg ttatgttcgt cggctttttt atattcatca tgagcggata
      721 tcccgtcggc tactcattcg ctgccactgc cattcttttt ggcggcattg gctacgccct
      781 cggcgcattc gacctcaacc tgctcaaact tctgccaagc cgttggtttg gcaccatgtc
      841 cgatttcacg ttgttggcaa ttccattttt tattttcatg ggcgcggtct ttgaaagatc
      901 ggggttggct gaagaacttc tcgaaacagt cggcaggctc atgggtaggg tcagaggcgg
      961 gctggcactg gcagtcatcc tcgttggcac gctcctcgcc gccacaacag gtgtcgttgc
     1021 agccaccatc atcatgatgg gcatgcttgc actcccaacc atgctccgac acggctacga
     1081 caaacaactc gccacaggcg tcatcaccgc ttcaggcaca ttggcgcaaa tgatcccgcc
     1141 cagcctggta ttggtggtat tgagcgatca gatcggcgtg cctgttggag atttattcct
     1201 cggggcagtg atccctggat tgttattatc ttcgctgtat gctttatacg tcatcatcat
     1261 cacccgcatc aaacccgacc tcgcacctgc tctgccgcct gaagcatttt ccgtcactgg
     1321 agcagacttg ctccgaaaat ccgtgcgcgt catcattccg cctgggttgt tggtcttctc
     1381 tgtgctggga agcattttct tcgggctggc ctcgcccacc gaagcaggca gtgtcggggc
     1441 tgtcggcgcc tgcctgctgg cattgatgaa caaacgactc accacaaaac tcattcaaga
     1501 tgcagccatg tccactgcac ggttgaccgc gctcgtcatc acaattttat tcagctccac
     1561 ttttttcagc ctcgtcttcg atggccttgg cgggcaaagg ctcatcaccg acctgctggt
     1621 caacctgcct ggcggattct ggggtttcat catcgtagcc aacattgcca tctttttact
     1681 gggtgtcttt cttgagttcg tcgaaatctg cttcatcgcc atgccgcttt ttgtccctgc
     1741 cgcgcatgcg ctcggcattg acatggcctg gctggcaatt gtcttcgcca ttaacctgca
     1801 gaccgctttc atctccccgc ctgttgggtt ttcgctgttt tacctgcaaa ccaccgcgcc
     1861 aaaagaagtg cccacttccg acatccacaa aggcgcattg cccttcatgg ctttgcaggt
     1921 tctcgcatta ataatcgtta ttattttccc ccaaaccgtc acctggttga tcgagctctc
     1981 tgcaaaaagc ctgccataaa ataaaaactg gcacaaaagc gaaaatgctt ttgtgccagt
     2041 tcaattcccg tttcctgttt acagccccta ccctgccata aacttcaaca acgcaaactc
     2101 attggtgaag ctccactttt gaattcgagt gcggaactcc atccattttt catagacggc
     2161 cttgaattca gcatcctctg cagatttggc atcgtaaagt tcaaaagcct tttcctgcgc
     2221 ggcctgcatg atctcatcac tgtacgacat cagcttcacc ccgcccgcca gcagacgttc
     2281 caacgcctcg ccgttgaccg cgtcataatg agccgccatg gcaacattgg cctcatagct
     2341 cgcggctttg atcgcttcct gatacagggc aggcagtttg ttccactcat ccagattgat
     2401 ctgcaattca agcgatggac ctggctccca ccaccccggg aagtaataaa agcccgtcgc
     2461 cttgtgcaag ccgagttttt catcatcata cggacccacc cactccgctg catcaattgc
     2521 gcctgtttcc aacgcctgga aaatctcgcc gccggggatg ttctgcacct gcacacccaa
     2581 agccgacatt acctgcccac ccaaccccgg gatgcgcatt ttcaatccct gcaggtcggc
     2641 gagggtgttg atctccttgc ggaaccagcc gcccatttgt gcgctggtat tgccagcagg
     2701 gaattgaatg acattgaatt tcttcgcgta aaaatcctgc aataattcaa gtccgccgcc
     2761 gttgtagagc cacgcatttt gctgtgacgc ggtcaaacca aacggcaggc ttgtgccaaa
     2821 ggccagcacc cagcttttac caatgtaata ataggaagcc gtgtgaccga tcggcaccgc
     2881 accggacgac accacatcca gcacttcggt ggcttttgca agttctccgc cagcgcgtgg
     2941 tgtgatggtg aatttgccgc ccgtcaacgc tttgacacgt tcagcaaatt tctgtgttgc
     3001 tccgaacaaa atgtccaaac ctgtgctcca actggtcgcc atttgccaat ccagcacggg
     3061 caggtcttcg gcggcggcat taccagcggc aggggcgggt tctgcacagg cggcaagtgc
     3121 ggaagccgct acacccattc cagctacttt taagaagtct cttcggttca tgtcacactc
     3181 tctcctttgg tttgttttgc ctgattgtat cgtatgc
//
LOCUS       DAOQLC010000268         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16514_length_3196_cov_13.287806, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000268 DAOQLC010000000
VERSION     DAOQLC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16514_length_3196_cov_13.287806"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="PL000_21495"
     CDS             <1..2147
                     /locus_tag="PL000_21495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91514.1"
                     /translation="PLLLRADGNTAAVNVSGYPLTFNGGDVTIRSRGQTLHRINVGFF
                     GTVTGAREGLTINNVGNWVLDANGQASASGGAAAGGIIQVKADQSSLIALDSATDPSS
                     GNIVIRANGDDANGDGGIISVDTKSFVLGDTTRAVVSANGAPNGTGNAVTAPLESAGP
                     FAVTFKSGSTNMIFGGGNKAVKITATGGKTSGNGGTIKVLPSTGTITVRNSLALDASA
                     IGNNCGTSCDAGQIQVVASGEIKANTSTTAPVVPAIKANGAGSGTGGKIILQSTGDVV
                     TEGSIEANGGCDGGDGGEITLTGENVKTLGESSASGGDGSFCLAVAGSPNSFAARLAP
                     GSANGGKKTINANDKWLNGPLTALHFDGKGDGKGGEIIINNPNKQVDLSPLPKDGITA
                     RAGKDGKGIGGKVTIASAKGAQNSLIDVNRIIKVDGGSGVDAANDSFGKISLNGVSCE
                     QWKTGFQNAFPATYWNCIDGARATGMPVAIAANSLPNGLRSLLGETKDQQNHPSVQIY
                     VMASIDSWADFFKTADAGAGGVYGISSSSIRVSAAFANVYSSGALISSTAAFPPASSG
                     SATVMQATMLHELGHQLDYIWGDITSNAQSPYNAAYLQARTDDLTRLLNSGACSNAFT
                     VATCNANAASWAGLTNLQIYGSGNPAANPPIKGLGYSTSNLELLPIVFGHLMGAGQYG
                     VAPDLENVVQTQFTDLSAFVQGLINNPPPAVQ"
     gene            complement(2192..2719)
                     /locus_tag="PL000_21500"
     CDS             complement(2192..2719)
                     /locus_tag="PL000_21500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91515.1"
                     /translation="MSPYFTKILCLLAVASSLYGPVRAEQKSPHSAYTLETFCILPED
                     IAKIEPAKDPSDCAVRPTTLCRFPCKRELWVKNGNRCVAIVRVEPSNSAVLNPPGFNY
                     GPIPALNMLRESDADVLWGTKTKQKIGPVVKYTLPTYQSADAITIETIFENSLLKKYR
                     IVGISIPSAAWNTVE"
BASE COUNT          792 a          763 c          816 g          825 t
ORIGIN      
        1 cgccgctctt gcttagagcg gacggcaata ccgccgcagt gaacgtttca ggatatccgc
       61 tgacattcaa cggcggggat gtgacaattc gctcgagagg gcaaacactt catcgtatca
      121 atgtcggttt ctttggcact gtaaccggcg ccagggaggg cttgaccatc aacaacgtcg
      181 gcaactgggt cctggacgca aatggacagg cttcggcttc tggcggtgca gccgcaggcg
      241 gtatcattca ggtcaaagca gaccaaagct ctctgattgc tcttgattct gctactgacc
      301 catcgagcgg caatatcgtc atccgtgcga atggcgatga cgcaaacggc gacggcggca
      361 ttatctcagt cgataccaag tcctttgtac ttggcgatac tacacgagct gtagtcagtg
      421 ccaacggtgc tcccaatggc accggtaatg ctgttactgc tccgcttgag tcggctggtc
      481 cttttgctgt caccttcaaa agtggttcaa ccaacatgat ttttggcggc ggaaacaagg
      541 cagtcaaaat caccgctacg ggtggcaaga catctggtaa tggcggcact atcaaggttc
      601 tgccgagcac tggaacgatt actgtcagga actctcttgc tcttgatgca tctgcgattg
      661 gcaacaattg cgggacttca tgcgatgctg gacagattca ggtcgttgct tccggcgaaa
      721 tcaaggcaaa caccagcact accgctccag ttgtcccggc cattaaggct aacggcgccg
      781 gtagcggcac tggtggcaag attatccttc agtcaaccgg cgatgtagta accgaaggct
      841 ctatcgaagc taatggcggc tgtgatggtg gcgacggcgg cgaaatcact ttgaccggtg
      901 aaaacgtcaa aacccttggc gagtcttccg cttcgggtgg tgatggcagc ttctgcttag
      961 ctgttgctgg ctctcctaat tccttcgctg ctcggctcgc acccggaagt gccaatggtg
     1021 gcaagaagac cattaacgcc aacgacaaat ggttaaatgg acctcttact gctcttcact
     1081 ttgacggcaa aggtgatggc aaggggggtg agattatcat caacaacccg aacaaacaag
     1141 ttgatctttc accgcttcca aaagacggaa ttactgccag agctggtaag gatggcaaag
     1201 gaattggcgg caaagtcacc attgctagtg ccaagggtgc ccaaaatagc ctgattgatg
     1261 tcaatcggat tatcaaagta gatggcggct ctggggttga tgctgcaaac gattcgtttg
     1321 gcaaaatcag cttgaatggc gttagctgtg agcagtggaa aaccggtttt caaaacgctt
     1381 ttcccgccac ttattggaac tgcattgatg gtgcgcgggc aaccggaatg cctgtagcta
     1441 tcgcagcgaa cagtctgccc aatggactga gaagcttgct tggtgagact aaggaccagc
     1501 aaaaccatcc tagcgtccag atctacgtga tggcaagcat cgattcttgg gcagactttt
     1561 tcaaaactgc cgatgctggt gctgggggcg tatatgggat cagctccagt tccattcgcg
     1621 tcagcgcagc ttttgcaaac gtctatagtt ctggtgcgct gatttcttct acggctgctt
     1681 ttccccctgc tagttccggc agcgccacgg taatgcaagc tacgatgttg catgaactgg
     1741 gtcatcagtt ggattacatc tggggggata taacctccaa tgcccaaagt ccctacaatg
     1801 ctgcatatct gcaagctcgc actgatgatc tgacaagatt gctgaactca ggcgcttgca
     1861 gcaatgcgtt cactgttgca acttgcaatg ctaacgctgc ttcatgggca ggactcacga
     1921 atttgcaaat ctatggttct ggcaatcccg cagccaaccc acccattaag ggactcggct
     1981 attcaacttc taatctggaa ctccttccta ttgtgtttgg ccatctcatg ggggctggcc
     2041 aatacggagt cgcaccggat ttggagaatg ttgttcaaac tcaattcact gatttgagcg
     2101 catttgtcca aggattgata aacaatccgc ctccagcagt tcagtaacta agctgggaca
     2161 ggaggtaggt attacctacc tcctgttttt tttattcaac agtattccag gcggccgaag
     2221 gaatcgaaat ccctacgatc ctgtattttt tcagtagtga gttttcaaaa atagtttcaa
     2281 tcgttatagc atccgctgat tgatatgttg gcaatgtata cttcacaacc ggtccaattt
     2341 tttgtttggt ctttgttccc catagtacgt cagcatctga ctctcggagc atattcagag
     2401 ctggaattgg gccatagtta aagccaggtg gattcaagac tgcactatta ctaggctcaa
     2461 ctctaacgat tgctacacaa cgattgccat tcttgaccca gagttctctt ttacaaggaa
     2521 atcgacaaag ggttgtgggc cgaacagcgc aatcggatgg atcttttgct ggctcaatct
     2581 tagcgatgtc ttcgggaagg atacaaaacg tctctaaagt gtaagcagag tgtgggcttt
     2641 tttgctcagc cctaaccgga ccatataagg atgatgctac agccaaaagg cacaatattt
     2701 ttgtaaaata cggactcata tagccaactc cttaatgggc tctgtagtaa ataataccag
     2761 gaaaaattga gatgagtttt gagacgtgtt tctctgagcc gttcttttgg ggtttagttg
     2821 taagtggctc ggttggagcg aaggtgtcac caccggaaaa gtcaagaggt agccgagcaa
     2881 agtaccgtgt tttgaattgg tgacggaaaa tcggcataaa agctgaggaa ctttgggctt
     2941 ttgaggtctt agcaatgttc ttttttgcct ctaaagtgcc gactccagca caagtaggaa
     3001 catcgtctaa agccggtttc agaattactg ctgcatcgag ggcaaatgga actagattag
     3061 ctacctgtct attagagtct cgaaagcccg cccctgttac tttttttgcc tttagctacg
     3121 atggtccgta gtataaaagg cggttaggac acctcctgcc ctagcaacgg gctacggagt
     3181 agcaggataa agctgc
//
LOCUS       DAOQLC010000269         3188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16562_length_3188_cov_21.443345, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000269 DAOQLC010000000
VERSION     DAOQLC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16562_length_3188_cov_21.443345"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /gene="mvaD"
                     /locus_tag="PL000_21505"
     CDS             <1..927
                     /gene="mvaD"
                     /locus_tag="PL000_21505"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HNC91516.1"
                     /translation="FIKYWGNRDNTYRLPSNSSISMNLDGLFTRTTVSFTSAKADSLA
                     INAAEVTGKGLDRVSYILDLVRKMANINERAEVTSENNFPAGAGIASSAAAFAALALA
                     ASKAAGLNLSEPELSVLARRGSGSASRSIPGGFVEWQMGREEADSYSFSIAPADHWNL
                     VDCVAIVNATHKKTGSTEGHAIAGTSPLQNARVADAPRRLEICRNAILQRDFEAFANI
                     IEHDSDMMHSVMMTSNPPLMYWQAATVEIFHQVREWRASGLPVGYTVDAGANVHVLCL
                     GDSAIEVEKRLREIPGVSNVLVAGVGGAAKIV"
     gene            1066..2178
                     /locus_tag="PL000_21510"
     CDS             1066..2178
                     /locus_tag="PL000_21510"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNC91517.1"
                     /translation="MVSGFVSLIVFLLALGGMIFVHELGHFIAARWAGVEVEEFGFGL
                     PSYRIAKLFTWQGTDFTIHALPLGGFIRPKGENDPNVPGGLASANPWKRLVVLAAGPF
                     MNLLTAVVVFSFLIASEGVPVTGSIKIDAVSDNSPAQAAGMLPGDILLAINGEKVTEV
                     DAAIAIIQANLDKPVELLMDRNGEEVTLTATPLSSRLKSEGALGIGLGYPTRPATVIE
                     SISGGFMITGAQAAMIVYLPIALIQGAIAPEEARFIGFKGIFDMFDVAVEEDVTTRQE
                     VAAATAGTPTPQPTNWTLNLIGILSISLGVMNLFPIPALDGGRILFTMPEILFRKRIP
                     SEWENMVNGIAMLLLICLMLFVNVMDFVNPAQIPTP"
     gene            2182..3183
                     /locus_tag="PL000_21515"
     CDS             2182..3183
                     /locus_tag="PL000_21515"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNC91518.1"
                     /translation="MTQIITLQTALEHLLNSNADIPKNHLQHYSDLDPQSLTIFMDAW
                     PRVKPTRKLLLLDELLSHLDEDTIVSYEEIGRALLNDANSDVRSRAIRLLAESDDPKL
                     ASKFIEILLNDPDLAPRMESATLLGEFILMGELEKLKQSLQQKIEGALISVIRSEDNP
                     SLRRRALEALSYSSNPEVAALIESAFERADPAWVAAALRSMGRSHDERWNEDVVSRML
                     DDDPRIRFAAVEAAGELNIEEAGPILIKMLEDEEEDDDVVAAAIWSLSQIGGDDARIY
                     LLNLIENTEEEELVEFLEDALENLDFNTELNKFDLLSLDDDDDLLELDEEELEDEDD"
BASE COUNT          744 a          829 c          850 g          765 t
ORIGIN      
        1 ttcatcaaat attggggcaa tcgcgacaat acgtatcgcc tgccgtccaa ttcttccatc
       61 tcgatgaatt tggatggatt attcacccgc acaaccgttt cgttcacttc tgcaaaagca
      121 gattcgctgg ccattaatgc cgcggaggtg acaggcaagg ggttggaccg tgtatcctac
      181 attctcgatc tggttcggaa gatggccaac ataaatgaaa gagcagaagt gaccagcgag
      241 aacaacttcc ctgccggggc gggaattgcc tcttctgcgg cggcgtttgc ggcattagcc
      301 ctggctgcca gcaaagcggc ggggctgaat ttgagcgagc ctgaactctc ggtgctggcc
      361 cggcgcggat cggggtccgc ttcacgctcc ataccgggtg gttttgtaga atggcaaatg
      421 ggaagggaag aagccgattc gtactctttt tccatcgcgc cagccgacca ttggaatctg
      481 gtcgattgtg tggccattgt caatgcgacg cataaaaaga cgggctccac ggagggccat
      541 gccattgctg ggacaagtcc gctgcaaaat gcgcgtgtgg cagatgcccc acggcgtctc
      601 gaaatttgcc gaaatgcgat tttgcaaagg gattttgaag cctttgccaa tatcatcgag
      661 catgattcag atatgatgca ttcagtgatg atgacctcca acccgccgtt gatgtattgg
      721 caggctgcca cagtggagat ctttcatcag gtgcgtgaat ggcgtgccag cggattgccc
      781 gtgggctaca cggtggatgc gggcgccaat gtgcatgtgc tgtgtttggg tgattccgcg
      841 atcgaagtgg aaaaacgact gcgtgagatt cctggtgtga gcaatgtgct ggtggctggg
      901 gtcggtggcg cggcgaaaat tgtttagggg agaaactgtc cttcgaaaca gagtcaggtt
      961 tcggttgagt atgaccgcgc ttttcgtcta ataaaagatt aacttcggca aatccatttg
     1021 aagatataat ccaatttgtc gtttaaacta ggcaaaggat aaaaaatggt atctggtttt
     1081 gtatctttaa ttgttttcct gctcgctttg ggcgggatga tctttgtaca cgaattgggg
     1141 catttcattg cggcacgttg ggcgggtgtt gaagtggaag aatttggttt tggtctgcct
     1201 tcctacagaa tcgccaaact gttcacctgg caagggaccg attttacgat ccatgcgctg
     1261 ccgctgggtg gttttattcg cccgaagggt gaaaatgatc caaacgtacc gggcgggttg
     1321 gcttcggcaa acccgtggaa acggctggtg gttctggccg ctggcccgtt catgaatctt
     1381 ttgaccgctg tcgtggtgtt ctcgtttttg atcgcttcag agggtgtgcc agtaactggc
     1441 tcgatcaaga tcgatgccgt ctctgacaac tcccctgccc aggcggcggg gatgctgccg
     1501 ggagatatcc tgcttgccat caacggcgag aaggtcaccg aagtggatgc tgccatcgcg
     1561 atcattcaag ccaacctcga taaacctgtt gagttgttga tggaccgcaa cggtgaagag
     1621 gtgactctca ccgccacacc gctttcaagc agattgaaaa gtgaaggcgc gctcggcatc
     1681 gggttggggt atcccacacg gcctgcaaca gtgatcgaaa gtatttcggg cggtttcatg
     1741 atcacgggcg cacaggccgc catgatcgta tatctaccca ttgctcttat tcagggtgcc
     1801 attgcgcctg aagaggcacg ctttatcggg ttcaaaggga tctttgatat gttcgatgtg
     1861 gctgtggaag aagacgtgac cacacgacag gaagttgccg ctgccacagc aggcacaccc
     1921 actccccaac ccacaaactg gaccctgaac ctgatcggca ttctgagcat ttcacttggt
     1981 gtgatgaacc tcttccccat ccccgcactg gatggcggcc gcattctctt caccatgcct
     2041 gaaatccttt tccgaaaacg catccccagc gaatgggaaa atatggtcaa tggcattgcc
     2101 atgcttctgc tgatctgcct gatgctcttc gtcaatgtga tggacttcgt caaccccgct
     2161 caaatcccca ccccataatc tatgacacaa attatcaccc ttcaaaccgc gctcgaacat
     2221 ttgctgaatt ccaatgcaga tattccgaag aatcaccttc agcattattc tgaccttgac
     2281 ccgcaatcgc tgactatttt tatggatgcc tggccgcgcg tcaaaccgac ccgaaaactg
     2341 ctcctgctcg atgagctttt atctcacctt gacgaagaca ccatcgtttc ctacgaagag
     2401 atcggtcgtg ccctgctgaa cgatgccaac agcgacgtac gttcacgcgc cattcgcctg
     2461 ctggccgaat cagacgaccc gaaacttgcc agcaaattta tcgagatctt actcaacgat
     2521 ccagacctcg ccccacggat ggaatctgcc accctgttgg gtgaattcat tttgatgggc
     2581 gagttggaaa aactgaagca aagcctccaa caaaaaatcg aaggagcgct gatttccgtc
     2641 atccgcagtg aggataatcc atcgctgcgc aggcgcgccc ttgaagccct cagctactcc
     2701 tcaaacccgg aagtggcagc cctgatcgaa tctgcttttg agcgcgcaga cccagcgtgg
     2761 gttgccgccg ctttgcgttc catgggccgc tctcacgacg aacgctggaa tgaagatgtc
     2821 gtcagccgca tgctggatga tgatccacgc atccgctttg ccgctgtgga agccgctgga
     2881 gaactcaata ttgaagaagc tggccccatc ctcatcaaaa tgcttgaaga tgaagaagag
     2941 gacgatgatg tggtcgccgc ggcgatctgg tcgctttcac aaatcggcgg cgatgacgca
     3001 cgcatctacc tgcttaacct tattgagaac accgaagaag aagaactggt cgaattcctc
     3061 gaggacgccc ttgaaaacct cgatttcaac accgaactaa acaagttcga cctgctatcc
     3121 ctcgatgacg acgacgatct tttggagctg gatgaagaag aactggaaga cgaagacgat
     3181 taatctgc
//
LOCUS       DAOQLC010000270         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16690_length_3173_cov_26.408275, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000270 DAOQLC010000000
VERSION     DAOQLC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16690_length_3173_cov_26.408275"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PL000_21520"
     CDS             <1..1082
                     /locus_tag="PL000_21520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC91519.1"
                     /translation="AIQRSLAGRARWRAQHDAQGRELREVMNEFDRQYDGSMELVRGQ
                     FHSIREDIGQTYKIIGDATANLVGGTSQQSASRMNMIYKLVDNLVEVTSGNQQHDQTA
                     GIKRYTDDTERIVNQLVGYMTEVRGAGEQAAISFAQMDQLISSVIQFLNKVNEITKQT
                     DLLALNAAIEAARAGEAGRGFAVVADEVRKLSHNTGEFSTEIHNMLSQIEISMRVVEH
                     SIKRVSQLDMSVADESRRNVEQMRTEMENLNVAAAEQSRHIVDASGQMQTLLMEGIVS
                     LQFDDLVSQSLDHIKRRSDVLEHYLDSLYAVQRDQSERDGLTRFRNRIANLSKVVTGV
                     RGQIEALGHKPVQQDNLDAGSVDLF"
     gene            1180..2718
                     /locus_tag="PL000_21525"
     CDS             1180..2718
                     /locus_tag="PL000_21525"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC91520.1"
                     /translation="MKPVKHPSEIAREALKLLAARKLAPTPPNYQECYYEIAQTPNLQ
                     RFPEDHLRQIALALTAQNRAQQEQLDQLDVAIRRRSWQGVKDALTEFVKQAAASGSNG
                     KALDLRAVPEVASISAREFLPKLASLIESVRPALAGDDEGFAEQIAGLLQTLRNPRAD
                     IREVSNELNIFSRRLSFAAEEQVEIKQALLRLLHVIIENISKLMLEDTWLEGQIAALL
                     DAVNPPLSLRRLDDVERRIRDVMYKQAAAKERSLEAQAEIRQMLGTFIERLSAMNESS
                     TTFQGRMEDSARQLEQVKTLEDLVPLLKEVIGATRAMAEDTARTREELGSLQQHVLAT
                     EAEIAKLHLDLNNASAMARHDPLTNTLNRKGLDEVMVREIADMQRKGTSLSLALLDVD
                     NFKKLNDQLGHTMGDAALVHLVRVVRECMRPSDSLARYGGEEFVILMPDTPLEEAITA
                     MTRLQRELTKNFFLAGNEKVLITFSAGVAQLATDESQEGALKRADEAMYLAKRSGKNR
                     VVAG"
     gene            2725..3042
                     /locus_tag="PL000_21530"
     CDS             2725..3042
                     /locus_tag="PL000_21530"
                     /inference="COORDINATES: protein motif:HMM:NF022777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3135 domain-containing protein"
                     /protein_id="HNC91521.1"
                     /translation="MPGNDFLQQQDGANDQDWVWSMALYEHLCSLSRDDPQKFEQERA
                     RLIEEFISRSSNQKLRWLQMRIDFMRRHTHSPLKVCINLSNMMWQSVDDMKRRLIELR
                     DNE"
BASE COUNT          790 a          832 c          924 g          627 t
ORIGIN      
        1 tggcaataca acggagcctg gctgggcggg cgcgctggag agcgcaacat gatgctcagg
       61 gaagagaact tagggaggtg atgaacgagt ttgaccgcca atatgacggc tccatggaat
      121 tggtgcgggg ccagttccat tcgatccgcg aagacatagg ccagacgtac aagatcatcg
      181 gcgatgccac ggccaacctg gttggcggga caagccagca atccgcaagc aggatgaata
      241 tgatctacaa gttggtggat aacctggtcg aagtgaccag cggcaaccaa cagcatgacc
      301 aaactgcggg catcaagcgc tacaccgatg atacggaaag aatcgtcaat cagcttgtgg
      361 gttacatgac tgaggtacgg ggcgcaggtg aacaggctgc catcagcttc gcccagatgg
      421 accaactcat tagctcggtg atacagttcc tgaataaggt taacgagatc accaagcaga
      481 ccgatttgct agcgttgaat gcggccatcg aagcggcccg agcgggagag gctgggcgcg
      541 gctttgccgt ggtggcagat gaagtccgta aactgtccca caacaccgga gaattcagta
      601 ctgagatcca caacatgctg agccagatcg aaatcagcat gagggtagtg gagcattcca
      661 tcaagcgggt gtctcaactt gacatgagcg tggcggatga gtcccgcagg aacgtggagc
      721 agatgcgcac ggagatggaa aacctgaacg tagccgccgc cgagcaaagc cgacacatcg
      781 tcgatgcgtc aggacagatg caaacgctac tcatggaagg catcgtgtcc ttgcagtttg
      841 atgatttggt cagtcaatcg ctggaccata tcaaacgaag gtctgacgtg ctggaacact
      901 acctggatag tctgtatgcg gtccagcgtg atcaaagcga gcgcgatggt ttgacgcgct
      961 tcaggaaccg gattgccaat ctcagcaaag tcgtaacagg tgtgcgcggc cagattgagg
     1021 ccttgggtca caaacctgtc cagcaggaca acctggatgc aggcagtgtt gacctgttct
     1081 agcgaaaaac acccgaaaaa gctgcttcct ggcactcatg acgatgagtg ttgcatttcc
     1141 cggccccccc gccagacctg aaagcgagca acctttccga tgaaaccggt aaaacacccc
     1201 tccgagatcg cacgcgaggc gctcaagttg ctcgccgctc gcaaactggc cccgaccccg
     1261 cccaattacc aggagtgcta ctacgaaatc gcgcaaacgc ccaacttaca aaggtttcct
     1321 gaagaccatt tgcggcagat tgccctggcc ctgacggccc agaatagagc ccaacaagaa
     1381 cagctggatc agttggatgt cgccattaga cgtcgtagtt ggcagggtgt gaaagatgcc
     1441 ctgactgagt ttgtcaaaca agccgcagcc tctgggtcaa acggcaaggc cctggatctt
     1501 cgggctgtgc cagaggtggc aagtatttca gctagggagt tcctgcccaa gcttgcatcc
     1561 ctcattgaaa gcgtacgacc tgccctggcg ggtgatgacg agggttttgc cgagcagata
     1621 gcggggttgt tgcaaaccct ccgcaacccc agagcggata tacgggaggt cagcaacgaa
     1681 ctgaatatct ttagccggcg cttgtccttt gctgccgagg aacaagttga gatcaagcag
     1741 gctttgctca gattgctgca tgtcatcatt gagaacatca gcaaattgat gctggaagac
     1801 acctggctag agggccagat cgctgcactg ttggacgcgg tgaatccgcc gctcagcctg
     1861 cgccgcctgg atgatgtgga acgccgcatt cgcgacgtga tgtacaaaca agcagcggcg
     1921 aaagaacggt ctctcgaggc acaagcagaa atacgccaga tgttggggac gtttatcgag
     1981 cggctttcgg cgatgaacga gtccagcacg acattccagg gcaggatgga ggacagtgcc
     2041 cggcaacttg agcaggtcaa gacactcgaa gacctcgttc ccctgttaaa agaagtgatc
     2101 ggcgccactc gggccatggc cgaggatacg gccagaacgc gcgaggaact cggttccctg
     2161 caacaacatg tcctggccac cgaagcggaa atcgccaagc tgcatctgga cctgaacaat
     2221 gccagcgcca tggcacgcca cgaccccttg accaataccc tgaatcgaaa gggcctggat
     2281 gaggtcatgg tgcgcgagat cgccgacatg caacgcaagg gaacatccct gtctctcgcc
     2341 ttactggacg tcgacaactt caagaagctc aacgaccagt tgggacacac gatgggggat
     2401 gcggcgctgg ttcacttggt gagggttgtg cgcgagtgca tgcgaccctc ggattccctg
     2461 gcccgctacg gtggggagga atttgtcatt ctgatgcccg acacgccgct ggaagaggcc
     2521 attacagcca tgacccgtct acaacgggag ctgaccaaga atttctttct tgcaggtaat
     2581 gaaaaggtct tgatcacctt cagtgccgga gttgcccagt tggctacgga cgaatcccag
     2641 gaaggtgcgt tgaagcgggc tgatgaggcg atgtatctgg caaagcgttc tggcaagaac
     2701 cgcgtcgtgg ctggctgatt cgatatgccc ggcaacgatt tcttgcagca gcaggatggg
     2761 gccaatgatc aggactgggt ctggagtatg gccctgtatg agcatctgtg tagtctcagc
     2821 cgtgatgacc ctcagaaatt tgagcaggaa cgcgctcgac tgatcgagga attcatctct
     2881 cgttcaagca atcagaagtt acgctggctt cagatgcgca tcgacttcat gcgccgtcat
     2941 actcacagcc ccctgaaggt gtgcataaac ctgtccaaca tgatgtggca gtccgtcgat
     3001 gacatgaaga gacgcttgat cgaactcaga gataatgaat agctgtaatg gccgattgat
     3061 gacggtctgg tttggatttc atctggccga agttccagct aaaaaaacat tgaatttcat
     3121 tgcacactga ggcgcctgcg gtcagtcaat tccggttgct gcagcccttt gcg
//
LOCUS       DAOQLC010000271         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_16942_length_3148_cov_26.933721, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000271 DAOQLC010000000
VERSION     DAOQLC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16942_length_3148_cov_26.933721"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /locus_tag="PL000_21535"
     CDS             complement(<1..1553)
                     /locus_tag="PL000_21535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HNC91522.1"
                     /translation="MAIIPNIELETKLTLHRLLPRLEKTFEHSISTDPTGWAEFQMRL
                     KRHFPKLFNLYVEIYNERYDLFFHLEDLVTSLARSWFQRPTDLRALDNAREENPLWFQ
                     SNQMLGGVCYVDLFAEDLEGVKSKIPYFKELGLTYLHLMPLFKAPEGENDGGYAVSSY
                     REVHPPLGTMEELASLAHELRNAGISLVVDLVFNHTSDEHLWAQGAKSGDDEFQDFYR
                     IFPNREMPNLYEQNLREIFPDEHTGAFTFFPNLFKDGGWVWTTFHSYQWDLNYANPAV
                     FNRMAEEMLFLANQGVEVIRLDAVAFIWKQLGTNCENLPGAHVLIQAFNAVARIASPA
                     LLFKSEAIVHPDDVVKYISPEECQLSYNPLLMALLWNSLATRKIKLLSQALATRFKIN
                     PNTAWVNYVRVHDDIGWTFSDEDAAAFGINGNDHRRFLNEFYRGRFEGSFSRGLPFQE
                     NPKTGDCRISGTCASLAGLEKALKEEGPNEVELAIRRIHLIHGVILTAGGIPLIYLGD
                     EIGTLNDYT"
     gene            complement(1556..3019)
                     /locus_tag="PL000_21540"
     CDS             complement(1556..3019)
                     /locus_tag="PL000_21540"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HNC91523.1"
                     /translation="MSDRFRPAFHFTPPSMWMNDPNGMVYFDGEYHLFYQYHPHSLVW
                     GPMHWGHAVSKDMVNWQHLPIALHPDEHGMIFSGSAIVDAHNTAGFGKEAMVAIFTYN
                     NHTYNDKKRVEDQNLAYSTDRGRTWTKYAGNPIVPHPGNLTDFRDPKVFWHADHWVMA
                     LAAGDRVLFYISADLKHWKQSGTFGNGEYGSTVGVWETPDLFKLTVGTTTQTRWVLTV
                     GVGKGGPAGGSGTQYFIGDFDGQTFTSEDPKETILWADHGADYYAPQSWNHEPNDRRI
                     MLGWMNNWQYATTIPASEFRGSFSLPRELSLTQTQNGIRLIQTPIPELQSLRGARTQY
                     QNELISTDNDLLHHIRSATLEIKAEFQWTGNTDSFGFQLRVGANEETVISYLVAEQML
                     VVDRTRSGRVDFNEGFARIHSAPLAPIRNTIRLHIFVDAASIEVFANDGILTLTDSIF
                     PSPDSRGIKLLAQGGSVCLNELDIFELGSIQSINTEE"
BASE COUNT          685 a          715 c          965 g          783 t
ORIGIN      
        1 taggtgtagt cgttgagggt gccgatctcg tcgccgaggt agatgagcgg aattccgccc
       61 gcggtcagga taaccccgtg aatgaggtgg atgcggcgga tggcaagctc cacttcattt
      121 gggccttctt cctttaaagc cttctccagc ccggcgagtg aggcacaggt gccgctgatg
      181 cggcaatcgc ctgttttggg gttctcttga aagggaagac cgcgcgaaaa actgccctcg
      241 aaacggcctc tgtagaattc gttcaggaag cggcggtggt cgttgccgtt gatgccaaag
      301 gcggcggcat cttcatcaga aaaggtccag ccgatatcat catggacgcg cacgtaattg
      361 acccaggctg tattcgggtt gatcttgaaa cgtgtggcaa gcgcttgcga aagcagtttg
      421 attttgcgcg tagcaagtga attccacagc agggccatta acagcgggtt gtaggagagt
      481 tgacattctt cgggtgagat gtatttcacc acgtcgtcag ggtggacaat ggcttcggat
      541 ttgaaaagca gggcgggtga tgcaatgcgg gcaaccgcgt tgaaggcctg aatcagcacg
      601 tgggcaccgg gtaggttttc acagttggtg ccgagctgct tccagatgaa ggcaacggca
      661 tcgaggcgga tcacttccac accttgatta gcgaggaaca acatctcttc ggccatgcgg
      721 ttgaagacgg cgggattggc atagttcagg tcccattgat aggaatggaa ggtggtccac
      781 acccagccgc catctttgaa caggttgggg aagaaggtga atgctccagt gtgttcatcg
      841 ggaaatattt ccctgagatt ctgttcgtag aggttgggca tctcacggtt ggggaagatg
      901 cggtaaaagt cctgaaattc atcatcgccc gattttgctc cctgtgccca gagatgttcg
      961 tcggatgtgt gattgaagac cagatcaaca accaggctga tgcctgcgtt gcggagttca
     1021 tgggcaagcg aggcgagttc ttccatggtg ccaagcggtg gatgcacttc gcgataactg
     1081 ctgacggcat agccgccatc gttttcgcct tcgggggctt tgaagagcgg catgagatgc
     1141 agataggtga ggccgagttc tttgaaatac gggatcttgg attttacgcc ttcgagatct
     1201 tctgcaaata gatcgacgta acagactccg cccagcatct ggttcgattg aaaccaaagc
     1261 ggattctcct cccgggcatt gtcaagcgca cgcaggtcgg tggggcgttg aaaccacgag
     1321 cgtgccaggc tggtgacgag gtcttcaaga tggaagaaga gatcgtaacg ttcgttgtaa
     1381 atttcgacat acaaattaaa taattttgga aaatgccttt tcagccgcat ttgaaattcg
     1441 gcccagcctg tgggatcggt ggaaatggaa tgttcaaagg ttttttccag gcggggaaga
     1501 aggcggtgca gggtgagttt tgtttcgagt tcaatgttgg ggatgatagc catggttatt
     1561 cttctgtatt aatggattgg atggagccga gttcgaaaat gtcgagttcg ttcaagcaca
     1621 cggagccgcc ctgcgcaagc agtttgattc cgcggctatc tggagagggg aagatgctgt
     1681 cggtcagtgt gagtatgccg tcatttgcaa agacttcgat ggaggcggca tcgacaaaaa
     1741 tgtgcaggcg gatggtgtta cggatcggcg caaggggtgc ggaatggatt cgagcaaagc
     1801 cttcattgaa atcaacccgc ccggagcgag tgcggtctac aaccagcatt tgctctgcaa
     1861 cgagatagga aatgaccgtt tcttcattcg cgccgacacg gagttgaaaa ccgaatgagt
     1921 ctgtatttcc tgtccattga aattctgcct tgatctcaag tgttgcggat cggatgtgat
     1981 ggagcaggtc gttgtctgtg ctgatgagct cgttctggta ctgtgtgcgg gcgccgcgta
     2041 aactttgcag ttcgggaatt ggggtttgga tcaggcgaat tccgttttgg gtttgggtga
     2101 gcgacagttc gcggggcagg ctgaacgagc cgcggaattc ggaggcagga atcgttgtgg
     2161 cgtattgcca gttgttcatc cagccgagca tgatgcggcg atcgttcggt tcatgattcc
     2221 aggattgggg tgcgtaataa tctgcgccgt gatctgccca gaggatggtc tcttttggat
     2281 cttcggaggt gaaggtttgt ccatcgaaat caccgataaa gtattgagtg ccagaaccgc
     2341 ccgcggggcc gcctttgccg actcccactg tcagcaccca gcgggtttgg gttgttgtac
     2401 ccactgtcag tttgaaaaga tcgggcgttt cccaaacgcc tactgtggat ccgtactcgc
     2461 cgttgccgaa ggttccgctt tgcttccaat gtttcaggtc ggctgaaatg taaaagagta
     2521 cgcggtcacc tgcggccaat gccatgaccc agtggtctgc atgccagaat actttcgggt
     2581 cgcggaagtc ggtcaggttg cccgggtgcg gcacgatcgg gttgcctgcg tacttcgtcc
     2641 atgtgcggcc gcggtctgtg ctgtaggcga ggttctggtc ttcgacccgt tttttatcgt
     2701 tgtaggtatg gttgttgtag gtgaagatgg caaccatcgc ctctttgcca aacccagcgg
     2761 tgttatgtgc atccacgatg gcgctgccgg agaagatcat tccatgctca tcggggtgca
     2821 acgcgatcgg caagtgttgc cagttgacca tatctttgct caccgcatgt ccccagtgca
     2881 tgggtcccca caccaggctg tgcggatggt attggtaaaa gagatgatat tcgccgtcaa
     2941 agtacaccat gccattgggg tcattcatcc acatggatgg cggggtgaaa tgaaaggcgg
     3001 ggcgaaaacg gtcactcatg acgacaactt gatccaaaga taggaatacg gctgcagagt
     3061 gagtttttca ttcaaggcat aggcgcggtt ttcacacaga tcctgtccgc tcttttgata
     3121 ttcagcaggg atttgggcaa cttgaacg
//
LOCUS       DAOQLC010000272         3126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17164_length_3126_cov_21.886031, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000272 DAOQLC010000000
VERSION     DAOQLC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17164_length_3126_cov_21.886031"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PL000_21545"
     CDS             <1..253
                     /locus_tag="PL000_21545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC91524.1"
                     /translation="IKPDVILMDIQMPGMDGLEAIRRIRAFEDRSIASIPIIAVTALA
                     MRGDRELFMAAGANGYVSKPIKLNELVETIQSQLGGSKQ"
     gene            complement(291..1205)
                     /locus_tag="PL000_21550"
     CDS             complement(291..1205)
                     /locus_tag="PL000_21550"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNC91525.1"
                     /translation="MHKLIILSEYEKKYQALIEKAQLPNLQITGLLANDVDIAIGEPT
                     RLKAALAYLPGLKWAQSLWAGVEPLLDPALRRDYVLTNARGVFGGLMSEYVLGYLLAH
                     ERRIFQMAEDQKNKHWNDSDTGTLRGKTIGLLGVGSIGADVARTAKFFGMTVRGYTFS
                     SEASSDVDKYYHGNDLLEFAKGLDYLVNILPNTKDTHKIINADLLAALPPHALVINVG
                     RGPAVDEAALLEALSRQKVAGAVLDVYEKEPLPQDHPFWSTPNLHMTFHTAALSLPED
                     LTRIFLENYNLYNEGKPLKYQVDFEKGY"
     gene            complement(1208..2008)
                     /locus_tag="PL000_21555"
     CDS             complement(1208..2008)
                     /locus_tag="PL000_21555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91526.1"
                     /translation="MIELSLNEAQAARAIFAPLEHHTMIYALFENNLKARLFVDNASQ
                     PTAGMIAYKNRYAFGGDPNQSAFHADILQHFAETELPPRKGRAFLASFTTDAWIPTLQ
                     NLFPDDEVIVAPRLYLEITPEQNTPPALPDGFSLHAVTPELLASNIGGMELLEEEIHS
                     ERTSPEDFFANGFGICPVYENQIAGWCLSEYNTHDRCEVGIATLEPHQRKGIATTLTK
                     IFLNEAAQRGIRHVGWKCWERNEASVATARKAGFSLVHREQAVIVIYK"
     gene            complement(2008..>3126)
                     /locus_tag="PL000_21560"
     CDS             complement(2008..>3126)
                     /locus_tag="PL000_21560"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC91527.1"
                     /translation="GSNYAPVREKVMEMLQSCGPVTGLFHEYELKLAQKIQEHMPSIE
                     MFRMLGSGTEGVMAAIRAARTFTKKKYVIKVGGAYHGWSDQMVYGLHIPGTGRLEAKG
                     IPRGANARTREFYPNALGALKRQLWLNRLRGGTAAVIVEPVGPESGTRPVPKDFNQKV
                     RELCDEFGTLLIFDEVVTGFRLGLGGAQGYFNVKPDLTVFGKCITGGYPMAGGVGGRR
                     DVIATFAAGIGGVGERAYVGGTLSANPISCVAGYYAMVEMEKTNAAALAGKAGDRLTQ
                     GLQAIIEKYSLPFVAYNQGSIVHLETAGTMLLDLKHPIRLLKELKPRKHMMEEMGAAY
                     MANGLITLAGSRMYTSMADTDEVIDDALARFETVLKEC"
BASE COUNT          638 a          828 c          898 g          762 t
ORIGIN      
        1 gatcaagccg gatgtgatct tgatggatat tcaaatgccc ggcatggacg gtttggaagc
       61 catccgacgg atccgtgctt ttgaagacag atccatcgct tccattccga tcattgccgt
      121 caccgccctt gccatgcgag gcgacaggga acttttcatg gccgctggtg caaatggata
      181 cgtcagcaag ccaattaagt tgaatgaact tgtggagacc attcagtccc aacttggagg
      241 gtcgaaacaa taaaaaacag gaattgggta catgcccaat tcctgttttt tcaataccct
      301 ttttcaaaat caacctgata tttcagcggc ttgccttcgt tatacagatt gtaattctcg
      361 aggaaaattc tggtgaggtc ttcgggcagg ctgagggcgg cggtgtggaa ggtcatgtgc
      421 aggttgggcg ttgaccaaaa tggatgatcc tgcggaagcg gctctttttc gtagacgtcc
      481 aaaacggctc ctgcgacctt ttgcctgctc agcgcttcga gcagggcagc ttcatccaca
      541 gcgggaccac gaccgacgtt gatgacgagg gcatgcggcg gcagggcggc gagcaaatcc
      601 gcgttgatga tcttgtgcgt gtctttggtg ttggggagga tgttgacgag ataatcaagt
      661 ccttttgcaa attcgagcag gtcgttgccg tgataatact tgtccacgtc agaacttgct
      721 tcgcttgaga aagtgtaccc gcgcacggtc atgccgaaga atttcgccgt ccgcgccaca
      781 tccgcgccga tggagccgac gccaagcaag ccgatggtct ttccgcgcaa tgtgcctgtg
      841 tctgagtcgt tccagtgttt gttcttctgg tcctctgcca tttggaagat gcggcgttca
      901 tgtgccagca gatacccaag gacgtactca gacatgagtc cgccgaagac gccgcgggca
      961 ttggtgagga cgtagtctct gcggagggca gggtcaagca aaggctccac tcctgcccat
     1021 agagattgag cccacttcaa gccggggaga taggcgagcg cagctttgag gcgggtgggt
     1081 tcgccgatgg cgatatccac atcgttggcg agcagtcccg tgatctgtaa attcggaagc
     1141 tgcgcctttt cgatcaaagc ctgatatttt ttttcgtatt cagagaggat gataagtttg
     1201 tgcatggcta tttgtagatg acgatcacgg cttgttcgcg gtggacgagg gaaaagcctg
     1261 ctttgcgcgc agtggcaacc gaggcttcgt tgcgctccca gcacttccag cccacgtgac
     1321 ggatcccgcg ctgtgctgct tcgttgagaa aaattttggt gagtgtggtt gcgatgcctt
     1381 tgcgttgatg cggctcgagt gtggcgatgc ccacttcgca gcggtcgtgc gtgttgtatt
     1441 cggagaggca ccagcccgcg atttgattct cgtacacggg gcaaatgccg aagccgttcg
     1501 caaaaaaatc ttctggcgat gtgcgctcgg aatgaatttc ttcttccagt aattccatcc
     1561 cgccgatgtt gcttgccaac aattcgggcg tgacggcgtg cagggaaaat ccgtctggca
     1621 gggcgggcgg tgtgttttgc tcgggtgtga tctccagata caggcgcggg gcgacgatga
     1681 cttcatcatc gggaaaaaga ttttgcaggg tgggtatcca tgcgtcggtg gtgaaagagg
     1741 cgaggaacgc cctgcctttg cgcggaggga gctcggtctc tgcgaagtgt tgcaggatgt
     1801 ccgcatggaa cgcgctttga ttcgggtcgc cgccaaacgc gtagcggttc ttgtaagcga
     1861 tcatcccagc ggttggctgg gatgcgttat ctacgaatag cctcgccttg agattgtttt
     1921 caaacaaggc gtagatcatg gtgtgatgct cgagcggggc gaagatcgcg cgcgctgctt
     1981 gtgcttcgtt cagtgagagt tcaatcatta gcattccttc aaaacggttt caaagcgggc
     2041 gagcgcatca tcaatgactt cgtcggtatc tgccattgag gtgtacatgc gcgagcctgc
     2101 cagtgtgatg agcccgtttg ccatgtaggc tgcgcccatt tcttccatca tgtgtttgcg
     2161 cggtttgagt tctttcaata aacgaattgg gtgtttcaga tccagcagca tggtgcccgc
     2221 agtttcgaga tgcacgatgg agccctgatt atatgccacg aagggaagcg agtatttttc
     2281 aatgatcgct tgcagaccct gtgtgagtct atcgcctgct ttgcctgcga gcgcggcggc
     2341 atttgtcttt tccatttcga ccattgcata ataacccgcc acacaggaga tggggttcgc
     2401 tgaaagtgtg ccgcccacgt aagcgcgttc accgactccg ccgatgcccg ctgcaaatgt
     2461 agcgatcaca tcgcgccgac cgccgactcc acctgccatc gggtaaccac ccgtgatgca
     2521 cttgccaaac acggtgaggt caggcttcac gttgaaatat ccctgcgcac cgccgagccc
     2581 cagccgaaag cctgtgacca cttcatcgaa gataagcagc gtgccaaact catcgcacag
     2641 ctcgcggacc ttttgattga aatcctttgg aacgggacgc gtgccgcttt cgggtccaac
     2701 gggttcgacg ataaccgccg cggtgccgcc ccttagcctg ttcagccaaa gctgacgctt
     2761 caacgccccg agagcattcg ggtagaactc gcgcgtcctg gcattcgctc cacgcggaat
     2821 ccctttcgcc tcaagccgac ccgtgcctgg gatgtgcagc ccgtagacca tctggtcgct
     2881 ccagccgtga tacgcgccgc cgaccttgat gacatatttc tttttggtga acgtccgcgc
     2941 cgcgcggatc gccgccatca cgccttctgt ccctgagccc aacatgcgga acatttcaat
     3001 ggacggcatg tgttcctgaa ttttctgcgc gagtttcaat tcatattcat ggaacaaccc
     3061 agtgacaggt ccgcaggatt gcagcatttc catcactttt tcgcgcacgg gcgcgtagtt
     3121 gctgcc
//
LOCUS       DAOQLC010000273         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17345_length_3108_cov_22.701277, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000273 DAOQLC010000000
VERSION     DAOQLC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17345_length_3108_cov_22.701277"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PL000_21565"
     CDS             complement(<1..521)
                     /locus_tag="PL000_21565"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HNC91528.1"
                     /translation="MKKRVYIIYTGGTIGMQPTPEGYAPAPGYLAEQILSMPELKSNL
                     MPDVDIHEYDPLLDSANMTPEDWHKIASDIGAHYDDYDGFIILHGTDTMAYTASALPF
                     MLQGLGKPVVLTGSQIPLCEVRNDARANIITALTVAANFDIPEVCLCFGNQLLRGCRS
                     VKVSTDGLDAFASP"
     gene            complement(554..1876)
                     /locus_tag="PL000_21570"
     CDS             complement(554..1876)
                     /locus_tag="PL000_21570"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNC91529.1"
                     /translation="MSLKVGIDFGTSNSGVAIYDGQHVRLLPVDPKNVIPEVIKTVLY
                     VTREYRCYLGQEAVEAYYRDNVNRQRRFVKQWAGEIEYRGADLHYMRDIYVYVDELKP
                     GRLLQYLKTALRKEGYHGTQIFERFYTTGDLAKTYLSLLKRRAEDVLGEPIHAATLGR
                     PVKFSESAEQDKKAEDTLRQAAHEAGFKEVDFELEPIAAALDYEQAISKPQNVLIFDF
                     GGGTLDIAVMRLGDKNRKVYANGGVDIAGSDFDRTIIEKRMLPHFGHGRVKHHPEILE
                     MIHAVPDWMALPELGTPINRNILHKAIQAKIAPVRLKALEGLIYNDLAFTFYNRVEAG
                     KIALSKEGATVISLEDKNIALWDMYTRHQFETDIHHYLADVEKVLLDTIARSGLEPGQ
                     IDAVVKTGGSSNIPLFTGMLARIFGADKVKESNAFSSVVAGLAIKAWM"
     gene            complement(1873..3069)
                     /locus_tag="PL000_21575"
     CDS             complement(1873..3069)
                     /locus_tag="PL000_21575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91530.1"
                     /translation="MSFTKKIDEWMKEVEDRPESAITIVRLVVRRLRELSEHNEELLS
                     ENIALQTGTRVEEYQKRIAHLEYQLDMLKRRFGLDESALANSPTQMEGTASELNVLVY
                     NSLGQIFRTELSADTKEVGLIAGGISAELEPPRLLAVPSNENLLLLFTSGRVEKFSVT
                     DIPAMPLGSTWAWNQSALPHEPRAGEQLACIIPFSHLPLTDFLLQISRRGYAKKTLTN
                     ISESILNNHFIGKGTSQKSDQPFDLTLSRKNDIAALVTCEGKVTAFNVESLTYTTEER
                     IKLSATDYVIGSFILPADTSLLCVTQTGKVIHREGKSIEAAKTANTRGQVLIPPSRLE
                     QGVRFMGAVAVKDADKVLILDASGRINIHDVSTMTGSGSISADGLILSIGRISSEGGV
                     TRSVPS"
BASE COUNT          669 a          783 c          854 g          802 t
ORIGIN      
        1 ggggaggcga aggcatccaa gccatcggtg ctgactttga ccgagcggca tccgcgcagg
       61 agttgattgc cgaaacacaa acaaacttcg gggatgtcga aattcgcggc gaccgtcaat
      121 gcggtgatga tattggctct tgcatcgttg cggacttcgc acagtggaat ctgtgagccg
      181 gtgaggacga caggtttccc caatccctgc agcatgaacg gcaaagcgga cgcggtgtat
      241 gccattgtgt ctgtgccatg caaaataatg aaaccatcgt agtcatcata atgcgcgccg
      301 atatcgctgg cgatcttatg ccagtcttcg ggggtcatgt tcgccgagtc gagcagcgga
      361 tcatactcat gaatgtccac atcgggcatc aggttgcttt tgagttctgg catggaaagg
      421 atctgctctg ccaaatagcc aggtgcaggt gcataacctt caggagtggg ctgcatgccg
      481 atggtgccgc ctgtataaat aatgtaaaca cgttttttca tgagagcctc acctgatttt
      541 tattaattgc ttctcacatc caagccttga tcgccagccc agccaccaca ctgctgaagg
      601 cattggactc ttttaccttg tccgcgccga agattctcgc cagcattcca gtaaatagcg
      661 gaatgttcga tgagccgcct gttttgacga ccgcgtcaat ttgaccaggc tccagccctg
      721 atcgggcaat cgtgtcgagc aggacctttt ccacatctgc aagataatgg tggatgtctg
      781 tttcaaattg atggcgggtg tacatatccc atagcgcgat gttcttatct tcgaggctga
      841 tgacggttgc gccttctttt gaaagcgcga tcttgcctgc ctccactcgg ttgtaaaaag
      901 taaatgcgag gtcgttgtag atcaaacctt ccaacgcttt caagcgcaca ggtgcgatct
      961 ttgcttgaat ggctttatgg aggatgttgc gattgatggg cgttccgagt tcagggagcg
     1021 ccatccagtc gggcacggcg tggatcattt ccaaaatttc tgggtgatgt ttgactcgtc
     1081 catgcccaaa gtggggcagc atgcgttttt cgatgatcgt gcggtcgaag tcgctgcctg
     1141 caatatccac gccgccgttg gcatagacct ttctgttttt atcgccgagc cgcattactg
     1201 caatgtcgag agtcccgccg ccgaagtcaa agatgagcac gttttgcggt ttcgaaatgg
     1261 cttgctcata atccagcgca gccgcgatcg gttccaactc gaaatccact tctttgaatc
     1321 ctgcctcgtg cgcggcttgg cgcaaggtgt cttctgcttt tttgtcctgc tccgccgact
     1381 cggaaaattt tacagggcgt cccaaggttg ccgcgtgaat gggttcaccg agcacatcct
     1441 ccgcgcggcg cttgagtaaa ctcaaatagg tttttgcgag gtcgcctgta gtgtaaaacc
     1501 gttcgaagat ctgcgtaccg tgatagcctt ccttgcgcag tgcggttttg agatattgga
     1561 gcaaacgccc aggcttgagt tcgtccacgt aaacataaat atcgcgcatg taatgcaggt
     1621 ctgcaccgcg atactcgatc tcaccagccc attgttttac aaagcgccgt tgacgattca
     1681 cattgtcgcg gtaataggct tccacggctt cctgaccaag ataacagcga tattcacgcg
     1741 taacgtacag cacggtcttg atgacttcag ggatcacatt cttcgggtca acgggaagca
     1801 ggcgcacatg ctgaccgtcg taaatggcga cgccagaatt ggaagtgccg aaatcgatgc
     1861 caactttgag gctcatgatg gaacgctccg cgtcactccg ccctcagagc tgattcgccc
     1921 aatggacaga atcaacccgt cggctgagat cgatccgctg cccgtcatcg tgctcacatc
     1981 atggatgttg atccgccccg acgcatccag gatcaagacc ttgtcggcat ccttcactgc
     2041 aactgcgccc ataaaacgga ctccctgctc gagcctgctg ggcggaatca atacctgccc
     2101 gcgggtgttt gcggtctttg cggcttcaat acttttgcct tcgcggtgaa tgaccttgcc
     2161 cgtttgcgtg acacacagca gggaggtgtc ggcgggcagg atgaacgagc caatgacata
     2221 atcggtggcg gagagtttga tgcgttcttc ggtggtgtag gtaaggcttt ccacattgaa
     2281 ggcggtgact ttgccttcac aggttacgag cgctgcgatg tcattttttc gggagagggt
     2341 caggtcaaaa ggttgatcag atttttgcga ggtgcctttg ccgataaagt gattgttgag
     2401 gatcgattcg gatatgttgg tgagggtttt cttggcgtaa ccgcgacgac tgatttgcaa
     2461 aagaaaatct gtaagaggca ggtgtgagaa agggatgatg caggcaagct gttcacctgc
     2521 gcggggttcg tgaggcagcg cagattgatt ccatgcccag gtgctgccga gcggcatggc
     2581 tggaatatcg gtcactgaga atttttccac ccgccctgat gtgaaaagca atagcaggtt
     2641 ctcgttggat ggtaccgcca gaagccgcgg tggttcaagc tcggcgctta ttccgcctgc
     2701 gataagcccg acctcttttg tgtcggcgct taattcagta cggaaaatct gcccgagcga
     2761 gttgtaaacc aacacgttga gttccgaagc tgtgccttcc atttgggtgg gggagtttgc
     2821 gagcgcggat tcatccagcc cgaagcggcg tttgagcata tccagttgat attcaagatg
     2881 ggcgatccgt ttttggtatt cctctacacg ggttccagtt tgcagggcga tgttctccga
     2941 cagtaattct tcgttgtgtt cagagagctc tcgcaagcgc cgaaccacca gtctcacgat
     3001 ggtgatcgct gactcgggtc ggtcttcaac ttctttcatc cactcgtcaa tttttttcgt
     3061 gaaggacatg agaggttttc ctttcggggg aaataattat acttgacc
//
LOCUS       DAOQLC010000274         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17350_length_3107_cov_25.517366, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000274 DAOQLC010000000
VERSION     DAOQLC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17350_length_3107_cov_25.517366"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PL000_21580"
     CDS             complement(<1..241)
                     /locus_tag="PL000_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC91531.1"
                     /translation="MSPKSKPSVKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFCADV
                     GQGDEDLAGLEQKAIKSGASEFVIHDMKEEFVSEYL"
     gene            complement(560..1429)
                     /locus_tag="PL000_21585"
     CDS             complement(560..1429)
                     /locus_tag="PL000_21585"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNC91532.1"
                     /translation="MKIHLVDGTYELFRAHFGAPPKKAANGMEVGATLGLLRSMLLLL
                     SDPDVTHVAVAFDHVIESFRNKMYKGYKSSAGVDPVILNQFPLAERAVSALGLVTWPM
                     VKYEADDAIATAAAKFKKSKQVEQVVICSVDKDLTQMVDGDRIVCWDRRREIILNEKG
                     VVEKFGVGPKSIPDFLALVGDSADGYPGIQGWGEKSTATVLANYRQIEAIPSDPAKLG
                     LGLGRATTLLENLKNNYEDALLFRELSTLRTDVPLKETLADLKWQGATQRLKKVCKEL
                     GSESILDRIKIWR"
     gene            complement(1456..2025)
                     /locus_tag="PL000_21590"
     CDS             complement(1456..2025)
                     /locus_tag="PL000_21590"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC91533.1"
                     /translation="MRKIIVHEFITLDGVIQAPGSPTEDTDGGFTHGGWTIPYWHDDI
                     GAHFGQIFAEADTLLLGRKTWKLHGDAFEPNPAEDPFNGFKKYVVSSTLKSADGWRDS
                     TIISGDIIGEIRKLKNQPGGNIIMDGSSVLLHALFEHDLVDELSLHVYPLVLGSGKRL
                     FPSGKHISLKLIESSSLPTGIVYQRYQLA"
     gene            complement(2022..2636)
                     /locus_tag="PL000_21595"
     CDS             complement(2022..2636)
                     /locus_tag="PL000_21595"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HNC91534.1"
                     /translation="MKQGDPNNNNGFPKIGAPAMRALEAAGYTNLKQLTKVTEVELAQ
                     LHGMGPKALGILRETLKAQGLSFKQGKAGVTLKKKGSPVSRTDKVDEFLENLSHPLTA
                     EVEAVRSIIKGVNKDINEEIKWRAPSFNYKGEYLVTFNLWEEKRIHLVFHNPLISQVK
                     SSLLEGEYKNRRMAYFADMKDVKAKKPLLEKALKDLIKLLEKKP"
     gene            complement(2649..>3107)
                     /locus_tag="PL000_21600"
     CDS             complement(2649..>3107)
                     /locus_tag="PL000_21600"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNC91535.1"
                     /translation="EQPYVAVRVQVGIPFGEVLGGFWGEAGAYLASKGMKPSGPPFIR
                     YLTTDMDKKLDIEVGFPVDNIVDGNDRITTGVFPAGQYAVYLYFGHYENLVAVTAEFL
                     AWAEKNNITWQTTKKDEVEWWVGRIEWYPTDPDNEPDPQKWQTELAFLVK"
BASE COUNT          774 a          833 c          657 g          843 t
ORIGIN      
        1 aaagatactc actgacgaat tcttctttca tgtcgtgaat gacaaattcg ctcgcgccac
       61 ttttaatggc cttttgctcc agccccgcca gatcttcatc tccctgcccc acatccgcac
      121 aaaaacaaac gacttcgcag ccatagtttt ccttcaacca cggcacgatc acagacgtat
      181 ccagaccgcc cgagtaggct aagacgactt tcttgacact aggttttgac tttggactca
      241 tttttactcc ttgtatggta atgccgactt acccacaggg cttagtcggc gttacgaaaa
      301 ataaaaacag ccctccgaga aggagggctg tttgggttga cttgtgacta tgcaggctta
      361 gttcacattt gcgttcgttc cgccgtcaaa acaaaaaact cccgaccttc tctgggaagt
      421 gggcatggga cgacgggtac gaggcagtgc aaacaaattg aacatgttgg gcggtattct
      481 actcgaaatg tgaaatgagt caatggtttt gggcgggtaa attcaaagag tacccgaatg
      541 ggtactcttt ggaaatcatt caacgccaga tctttattcg gtcgaggata ctttcgcttc
      601 ccaattcctt gcaaactttc ttcaagcgct gtgttgcgcc ctgccatttc aagtctgcga
      661 gcgtttcctt caatggcaca tccgtccgca aggtcgataa ctctctgaaa agcagggcat
      721 cttcgtagtt attttttaaa ttctcaagca aggtggttgc gcgccccaga cccaggccaa
      781 gtttcgcagg atcgctggga attgcctcaa tctgcctgta atttgccagc actgttgcag
      841 ttgatttttc accccaaccc tgaatgcccg ggtaaccatc ggcagagtct ccgaccagcg
      901 ccagaaaatc cgggatcgac ttcggcccaa cgccaaattt ttcgaccaca cctttttcat
      961 tcaatatgat ctcacggcgc cgatcccaac acacaatgcg gtcaccatca accatctggg
     1021 tcagatcttt atcgaccgaa caaatgacaa cttgctccac ttgttttgat tttttgaatt
     1081 tagccgctgc tgtagcgatg gcatcatccg cttcatactt gaccatgggc caggtcacca
     1141 atcccaaagc agagacggcc ctttcagcaa gtggaaactg attcaagatg acggggtcca
     1201 cacctgcgct ggatttgtaa cctttataca tcttgttccg aaacgactcg atcacatgat
     1261 caaatgcaac agcaacatgc gtcacatcag ggtctgaaag cagcagcaac atactgcgca
     1321 gaaggccgag cgtggcgccg acttccatac cattcgcggc tttcttcggc ggtgcgccaa
     1381 aatgagcacg aaaaagttca taggtgccgt caacaagatg gattttcata caggccctca
     1441 tttactccgt gtttttcagg caagttgata tcgttgatac acaataccgg taggcagcga
     1501 agaagattcg atcaacttaa ggctgatatg ttttccgctt gggaacaagc gttttccgct
     1561 tccaagaacc agtggatata catgcagtga aagttcatcc accagatcat gctcaaacaa
     1621 agcatggagc aggacactgc tcccatccat aatgatgttc ccgccaggct gatttttcaa
     1681 cttgcggatc tccccgatta tatctccgct gatgatcgtt gaatcacgcc acccgtctgc
     1741 tgattttaac gtggaagaaa cgacatattt cttaaaacca ttaaatgggt cttcggcggg
     1801 gttgggctca aatgcatcgc catgaagctt ccatgttttt cgtcccaaca atagcgtatc
     1861 ggcttcagca aagatctggc caaagtgggc gccaatatca tcatgccagt atggaattgt
     1921 ccagccgcca tgggtaaaac cgccatcggt atcttcagtg ggactgcccg gcgcctgaat
     1981 gacgccgtcc aacgtaataa attcatgaac aataattttt ctcatggttt tttctccagt
     2041 aatttgatca aatccttcaa tgctttttcc agcaatggct tcttcgcctt cacatccttc
     2101 atgtctgcaa aatacgccat gcgccgattt ttgtactcgc cttccaacaa cgaactcttc
     2161 acctgtgaga tgagcgggtt atgaaagaca agatgaatac gcttttcttc ccagagatta
     2221 aaagtcacca ggtactcgcc tttgtaattg aaggatggcg ctctccattt gatttcctca
     2281 ttaatatcct tgttcacacc tttgatgatg gaccgcaccg cctccacctc cgccgtaagc
     2341 gggtgactga gattctcaag gaattcatcc accttatccg tgcggctgac gggcgagcct
     2401 ttcttcttca gcgttacgcc tgcctttcct tgtttaaaag acaagccctg cgccttcaac
     2461 gtctcgcgca aaatccctaa cgctttcgga cccatcccat gcagttgagc gagttccact
     2521 tccgtaactt tggtcaactg cttaaggttt gtgtatcccg ccgcctccaa ggcgcgcatg
     2581 gcgggcgctc cgatctttgg aaaaccattg ttattatttg gatcaccttg tttcatacaa
     2641 actcctgctt atttcacaag aaacgcaagt tccgtctgcc acttttgcgg atctggctca
     2701 ttatctgggt cagttgggta ccattcgatt cgtcccaccc accattcaac ctcatctttt
     2761 ttcgtagttt gccaggtgat attatttttt tcggcccaag ccagaaattc agcggtcaca
     2821 gccacgagat tttcgtaatg tccaaaatat aagtacacag catattgtcc agcgggaaat
     2881 acgcctgttg tgatgcggtc atttccatcg acaatgttgt caacaggaaa gccgacttct
     2941 atatcgagtt ttttatccat atcagtagtg agataacgaa taaaaggcgg accggaaggt
     3001 ttcattccct tgctggctaa ataggcaccc gcttcacccc agaagcctcc aagcacctca
     3061 ccaaagggta tccctacttg tacgcggact gctacatatg gttgttc
//
LOCUS       DAOQLC010000275         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17361_length_3106_cov_14.080957, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000275 DAOQLC010000000
VERSION     DAOQLC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17361_length_3106_cov_14.080957"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1427)
                     /locus_tag="PL000_21605"
     CDS             complement(99..1427)
                     /locus_tag="PL000_21605"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC91536.1"
                     /translation="MTEKKIIVSFKPLNWFKPNPKNTRTHSKAQIEYLASLIKEFGWT
                     NPIIARSNGLIIAGHGRLEAAQLLGLKNAPVIIKDFISASLYRAYSIADNQSALLSGW
                     DLDLLRQEIQDLSDKSFNVDLLGFDDSFIDSLLASGGSIDDSESVPDRPKIPKTKAGD
                     VYRLGPHTLVCGDSSDASVFTSIMAAGSCAMIWTDPPYNVDYGTRKRKIQNDNMAEEE
                     FKKFLLSCFSAFVPMLRKGGSIYVSHSDMHRNSFDYAWAASGLVLSSVLIWSKNSFIL
                     GRSDYQWQHESILYGWREGAAHRWYGGRKQATIQKFVDGESFRKTGDNEYTIIAGESI
                     FVIRGQSLSVREELSSIIYEPKPSRNGEHPTMKPPSLIARHVRNSSRKGDIVLDAFGG
                     SGSTLMACESTGRVAFLVEKDPAYCDVICDRWQAGGGVAPRRVHGKARNK"
     gene            complement(1781..2416)
                     /locus_tag="PL000_21610"
     CDS             complement(1781..2416)
                     /locus_tag="PL000_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91537.1"
                     /translation="MEIPFERFPEAIEEALNAFHWAGVTAMTRTAFKARAAAISAFKD
                     SFTIRAKTWPASQIRYQKATMDEQFAEVYTESKEMASQESGGARKDGSDYRVPVAAYD
                     VFGLNPNKVIPRQYRAKQIVGKTINGVTPYIETINGVSGVWAKTEDGVRLLYALSPEI
                     EIKAHPWFYASTEASIQATLEADYDKAVEEQMLKALSKNLEKGSGLFGAPE"
     gene            complement(2583..2834)
                     /locus_tag="PL000_21615"
     CDS             complement(2583..2834)
                     /locus_tag="PL000_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91538.1"
                     /translation="MSATLSDVRKKSPAYQAGAFFGMARSVANRVVPAASIIVSLDEM
                     LVRDLLQVMRSEKINCAWQKKGKSYILGVCVNSAVGRWN"
BASE COUNT          760 a          807 c          709 g          830 t
ORIGIN      
        1 ctgccccaaa cccgcgtttc gcatatccac cggaaggacc catttaaatg agcaatcaag
       61 tgccattgag aacgcatagg aacaacacag acgcatgatt atttattccg ggccttacca
      121 tgcaccctac ggggggctac ccctcccccc gcttgccatc ggtcacatat aacgtcacag
      181 taggcaggat ctttctcaac aaggaaagcc actcggcccg ttgattcaca ggccatgagg
      241 gtagagccag acccaccgaa ggcatcgaga acaatatctc ctttgcgcga tgagttgcgc
      301 acgtgacgag cgatgaggga aggaggcttc atggtgggat gctctccgtt ccttgaaggc
      361 tttggttcat agatgatgga tgatagttct tcgcgcacag agagagactg cccgcgaata
      421 acaaagatcg attcacctgc gatgattgtg tattcattgt ccccggtctt acggaaggac
      481 tcaccatcaa cgaacttctg aatggtggcc tgcttcctcc caccgtacca acgatgagcc
      541 gcaccttcgc gccatccata gaggatagat tcgtgttgcc attggtaatc ggaacgccca
      601 agaataaatg aattcttaga ccagatgagc acggacgaaa gaacaaggcc tgatgcagcc
      661 catgcgtaat caaaagagtt tctgtgcata tccgagtggg acacgtagat agatcccccc
      721 ttgcgaagca taggaacaaa cgcactaaaa caagagagaa ggaatttctt aaattcttcc
      781 tcggccatgt tgtcattctg aatctttctc tttcgcgttc cgtagtccac attgtaaggt
      841 ggatcggtcc agatcatcgc acatgaacct gcagccatga ttgaggtgaa gacagaagcg
      901 tccgatgaat ccccgcatac aagtgtgtgc ggacctagac gataaacatc gccggctttt
      961 gtcttcggta tttttggccg atctggaacg gactcagaat catcgatcga gcctccggat
     1021 gcaagcagtg aatcgataaa tgaatcatcg aagccaagca aatcaacgtt gaaactttta
     1081 tccgatagat cctgaatctc ctgacgcaaa aggtcaagat cccacccgga gagaagcgct
     1141 gattgattgt ctgcaattga atatgcgcga tagagtgacg cagagatgaa atccttgatg
     1201 atcaccggtg cgtttttcaa gcccagcaat tgtgccgcct ccaaacgtcc gtgccccgca
     1261 atgatcaaac catttgatcg agcaattatg ggattggtcc agccaaactc ctttatgagc
     1321 gatgctagat attcgatctg agcctttgaa tgcgtgcgcg tgtttttcgg atttggctta
     1381 aaccaattca gcggcttaaa cgatacgatg attttctttt cggtcatttc ttccttctga
     1441 cctttctgga aggaagcgca gtaagcgcat cgagcgagcg attcaaaaag ttcaaagatt
     1501 tggatcctgg ccttccgatt gcgtgcaatt gatactgacc agcgccctga tcgatcacca
     1561 gaagtgtagc ggcctcgtat ttattctttt cgcacaatct tctgactttc cgcgcgaacc
     1621 tttcgcgcct cctgatttgc ttctgctcgt cgctgaaata atttttcagg cgatccttca
     1681 gcgtgctcgt ttggtctaca tgcgacatca tcacttagct cctttgcaca ttggcattcg
     1741 atttctgaac atttggaccg atcattccag caaactataa tcattcgggc gcaccgaaca
     1801 accctgaacc cttctcaaga ttctttgaca gcgctttaag catctgctcc tccacagcct
     1861 tgtcgtagtc ggcctcaaga gttgcctgaa tgcttgcctc agtggatgcg taaaaccacg
     1921 ggtgcgcttt gatttcaatc tctggagata gagcatagag caggcgaacg ccgtcctccg
     1981 ttttagccca tacaccactc actccgttga ttgtttcaat gtagggcgtg acaccgttga
     2041 tagttttccc aacgatctgc tttgcgcgat attgtcgcgg gataactttg tttggattca
     2101 acccgaacac atcgtatgcg gccactggca cccggtagtc ggaaccatct tttctggctc
     2161 cgcctgattc ctgagaggcc atctcttttg attcggtgta cacttccgca aactgctcat
     2221 ccattgtcgc tttttgatag cggatctgcg aagcaggcca tgtcttcgcg cgaatggtaa
     2281 atgaatcttt gaatgcagag attgcggcag cgcgcgcttt gaatgcagtg cgagtcatcg
     2341 cagtaacacc ggcccagtga aaagcgttca atgcttcttc gattgcttcg gggaatcgtt
     2401 cgaagggaat ttccatgatt caaagtgctc cttaaattct ttgtgcggta gcgagcccat
     2461 ctcgcgggat tcagtgaaaa caaaatacac tgcctcaaat gatgaaccgg agcatttcgc
     2521 cactctgcgc ctaccagatt tttcaatcca gaaagtgccg ggcttgacgc cccaccattt
     2581 cctcaattcc accgtccaac ggcagagttc acgcaaacac caagtatgta tgatttccct
     2641 tttttctgcc aggcgcaatt tatcttttct gatcgcatca cttgcagtag atcacgcaca
     2701 agcatctcat caagagaaac tatgatcgat gcagcgggaa ccacacggtt tgcaaccgat
     2761 cgcgccatcc caaaaaaagc cccggcctga taagccgggg attttttacg cacatctgaa
     2821 agcgtagcgc tcatgactca ggcaatacct tctgcggatt cgcgtcaacc tttcttgtgg
     2881 ttggctttga ttttgccttt gcgttcttgg ttggtttttt ctttgttgtg accttttttt
     2941 tggtctgctt cttttttgcc atgctttgat ggactctaac ggaatgcgaa atacgtcaat
     3001 tgatttattg gattcaggat acaaaaccaa ccaggtgccc gcgatccaga gtaacaatcc
     3061 gtcgatgtct attctgcaca tctcccgtct agttttgcca tgcgat
//
LOCUS       DAOQLC010000276         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17604_length_3083_cov_12.877807, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000276 DAOQLC010000000
VERSION     DAOQLC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17604_length_3083_cov_12.877807"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PL000_21620"
     CDS             <1..1065
                     /locus_tag="PL000_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNC91539.1"
                     /translation="PAATEAPAATEAPADPMAELVAAAQAEGTLTTIALPHDWCNYGE
                     AIETFKAKYGIEVNELNPDAGSGDEIEAIKANKDNTGPQAPDVIDVGFAFGESSKAEG
                     LIQPYKVSTWDSIPDDAKDADGYWYGDYYGVLSFLVNTDVQPEVPQDWADLLDPKYNG
                     QVALSGDPRTSNQAIQTVYAASLANGGSLDDAQPGLDFFAQLNTAGNLVPLISNNGLV
                     ATGETAVRITWDYNALAAVDSFEGNPAAQVVIPATGRFAGVYVQAISAYAPHPNAAKL
                     WMEFLYSDEGQTIWMKGYCHPIREADMRERGVIPADLLEKLPDVSGAVFPSLAQLNAA
                     KELIVANWDSMVGADVQAAP"
     gene            1236..2126
                     /locus_tag="PL000_21625"
     CDS             1236..2126
                     /locus_tag="PL000_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC91540.1"
                     /translation="MAQTALNPKAPTPAMDWKKWFGVTPFFLFAILFLVLPSLRLVVG
                     SFTNSDGQFTFANVLQLFQEKSILDAYWLSIRISAATAIGGGIFGFLLAYSVTVGGLP
                     KPLRSALITFSGVASNFAGVPLAFAFIATLGRTGFVTALIKELFGVNLYQQTEFDLYS
                     FWGLSLTYMYFQFPLMVLIMAPALDGLKREWREAAENLGASTAQYWMRVAFPVLLPSI
                     LGSMILLFGNAFGAYATAYALTGGRLGIITIQIGAQIRGDVLHNPGLGYAMAMGMVVV
                     MTISLIGYSWLQKRSERWLR"
     gene            2123..2953
                     /locus_tag="PL000_21630"
     CDS             2123..2953
                     /locus_tag="PL000_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC91541.1"
                     /translation="MSIFARIRRIPFWSWFWFILGALYFLLPLYATVNFSLRMERDVI
                     GFKAYVRAFEDPEFLETFLYSNFLALMTIFASIILIVPTAYWIRLRLPEARRIVEFIT
                     LLPFVVPAIILVFGLIRIYSSPFKIPFTDITLVQPLTTFEVGTDILIVAGYMVLGLPY
                     MYRSVDTGMRTVDVRTLTEAAQSLGASWFTIITRVILPNIRAALLSGALLTFAIVVGE
                     VTLASFLGVPAFGPYLFLLGQHRAYEPAALSFASFLLTWAAMGLIQLFTGDQQVSGAH
                     "
BASE COUNT          628 a          805 c          758 g          892 t
ORIGIN      
        1 cctgccgcca ccgaggctcc tgctgctacc gaagctcccg ccgaccccat ggctgaattg
       61 gttgccgctg cccaggctga aggcacactc accaccatcg ctctgcccca tgactggtgc
      121 aattacggcg aagccattga aaccttcaag gccaaatacg gtattgaagt caatgaattg
      181 aatcctgatg ctggctccgg tgatgaaatc gaagcgatca aggccaacaa agataacact
      241 ggtcctcagg ctcctgacgt gatcgacgtt ggtttcgctt tcggtgaatc ctccaaggct
      301 gaaggcttga ttcagcccta caaggtttcc acctgggatt ccattcccga tgatgccaag
      361 gatgctgatg gctactggta cggtgactac tacggtgtgt tgtccttcct cgtcaacacc
      421 gatgttcagc ccgaagttcc ccaggattgg gccgacctgc ttgaccccaa atacaatggt
      481 caggttgcct tgtctggcga tccccgcact tccaatcagg cgatccagac cgtttacgcg
      541 gcttcacttg ccaacggtgg atctcttgac gatgctcagc ccggtttgga cttctttgcc
      601 cagctgaaca ctgctggtaa ccttgttccg ttgatctcca acaacggctt ggtcgccacc
      661 ggtgaaaccg ctgtccgcat cacctgggac tataacgctc tcgccgctgt ggattccttc
      721 gaaggcaacc cggctgctca ggttgtgatc cctgccactg gtcgtttcgc tggcgtgtac
      781 gttcaggcga tcagcgctta cgctccgcac cccaatgctg ccaagttgtg gatggaattc
      841 ctctattctg acgaaggcca gaccatttgg atgaagggct actgccaccc gatccgtgaa
      901 gctgacatgc gcgaacgcgg tgtcatcccc gctgatctgc tcgaaaaact ccccgatgtt
      961 tccggcgcag tcttcccctc cctggcccag ctcaacgctg ccaaggaact gattgttgca
     1021 aattgggaca gcatggtcgg cgctgacgtt caagccgctc cgtaacaaag tgattttggt
     1081 gtaataacgc gagaccctgc caactggcag ggtctcgcca tagcaagaga tattgccagt
     1141 tgcatacatt cctaaacttc tgagtgcaaa agaacggttc gaatattttg gtttagaaaa
     1201 ctatgcagta ccctgtaagt tgaggtgaat aaacaatggc tcaaactgct cttaacccaa
     1261 aagctccaac ccctgccatg gactggaaaa aatggtttgg cgtaacccca ttttttcttt
     1321 ttgcaattct atttcttgtt ttaccatccc ttcgtctggt tgttgggagt tttaccaaca
     1381 gcgacggcca atttacattt gcgaatgtgc tgcagttgtt tcaggaaaaa tccattctgg
     1441 atgcatattg gttgagcatt cgtattagcg cggcaacagc gatcggtggc ggtatttttg
     1501 gctttttgct cgcctactct gtaactgtgg gtggtttgcc caagccgctc cgctctgctt
     1561 tgatcacttt ttcaggcgtg gcttccaatt ttgcgggtgt tccgctggcg tttgctttca
     1621 ttgccacact tggccgcact ggttttgtca ccgcgttgat caaggaatta tttggggtaa
     1681 atttatacca acagaccgaa ttcgatttgt acagcttttg gggattgagc cttacctaca
     1741 tgtatttcca attcccgctt atggtgctta tcatggctcc tgctttggat ggtttgaagc
     1801 gtgagtggcg tgaggccgca gaaaaccttg gcgcaagcac agctcaatac tggatgcgag
     1861 tcgcatttcc agttttgctt ccttcgatcc ttggttccat gatcctgttg tttggaaatg
     1921 catttggtgc gtatgccaca gcctacgcac tgaccggcgg caggttggga atcattacca
     1981 ttcaaattgg cgcacagatt cgaggcgacg ttcttcacaa ccccgggctt ggttatgcca
     2041 tggcgatggg catggtcgtt gtaatgacaa tttccctgat cggatattcc tggcttcaga
     2101 aaagatcaga aaggtggttg cgatgagtat atttgcccga attcgccgta ttcccttttg
     2161 gagttggttc tggtttattt taggcgctct gtatttcctg ttgcctttat atgccacggt
     2221 caacttttcc ctccgcatgg agcgcgacgt gattgggttc aaagcttatg tcagggcttt
     2281 tgaagacccg gagtttcttg agacatttct ctactcaaac ttcctggcgt tgatgaccat
     2341 ttttgccagc atcattctta tcgtaccaac cgcctattgg attcggttgc gtcttccgga
     2401 agcccgccgc attgttgaat ttatcacgct tcttccgttt gttgttcctg cgatcatcct
     2461 ggtttttggt ttgatccgca tttacagttc cccctttaag atccctttta cagacattac
     2521 cctggtgcaa ccccttacca cctttgaggt tggtaccgat attttgatcg tggctgggta
     2581 tatggttttg ggcctgccat atatgtaccg ttcagtggat acaggtatgc gcactgtgga
     2641 tgtacgcaca ctgaccgagg cggcgcaaag cctgggtgcc agctggttta ccattattac
     2701 gcgggtgatc ctgcccaata tccgtgcggc gttgttgagt ggtgccttgc ttacctttgc
     2761 cattgtggtg ggagaggtta cccttgcctc atttttaggt gtgcctgcct ttgggcccta
     2821 tcttttcctg ctcggacagc atcgcgcata tgaacctgct gcactctcct ttgcaagctt
     2881 cctgctcaca tgggctgcca tggggctgat ccaactgttc acgggcgatc aacaagtctc
     2941 tggagctcat taaataaacg gaggaaaatt catcatgtca tatttgtcca tctcacaggt
     3001 tacaaagcaa ttcggcgata cggtagtggt tgatgatttc aatctcgata ttgaaaaggg
     3061 tgaattcgtt tcctttcttg ggc
//
LOCUS       DAOQLC010000277         3055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_17888_length_3055_cov_15.250333, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000277 DAOQLC010000000
VERSION     DAOQLC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17888_length_3055_cov_15.250333"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..378)
                     /locus_tag="PL000_21635"
     CDS             complement(64..378)
                     /locus_tag="PL000_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91542.1"
                     /translation="MQQPRPYWFLLDPYGWLILIMSIVTTFIVGLWWIVLVGIIGYLI
                     VLLVDLAGGRALGRTGSMRLARAEQENRELKAEQARLLGAIRERDEALKKTTPEKEET
                     KS"
     gene            complement(382..2181)
                     /locus_tag="PL000_21640"
     CDS             complement(382..2181)
                     /locus_tag="PL000_21640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC91543.1"
                     /translation="MDFSEKPKVSGSAIVVLIAVLLFGCIILGAFMIPTLKQMLQSNT
                     VPPAGATAVTDTTNATAAPPPGSVAIEISSSNTKEDWMNAVVEKFNAEQHKTADGNLI
                     FVTVKHVTSGGSQKDILNGKSQPTVWSPGDQSWVDEANTVWRDRTGQVLIPDQCAQTV
                     LAPIGFAMWKPMAQALGWPDTPISWDDIAALSADPKGWETLGHPEWGQFKFGHTHPDY
                     SNVGLLSMTALTYSTLGITEGLTADQVYSDAVVEAFTGVEQNTYHYGLQSRPLMQILA
                     QRGPEYLHAVTTSEAETLKTNKEFASTMRFPLVFIFPSKGTFWSEQPYCILNGEWVSD
                     EQKEAAATFRDYLLDKQQQEMAIDYYLRPIDTTIPLHAPLALDDGTDPRITMDTVPAL
                     QSPSADVSSAVKDVFHQTKKKSTIVLLLDISGSMEGEKIANAVSSSANFISRLDPNDE
                     IFLIVFGGGDGTVYSLGGGRAGDISEDLTTKLNGLYANGNTPLYDGVCTAIDKVNEVK
                     AEHDANNEKRLYGIVLLSDGQDTSSTRSQNQMFNCLPSGESVDGTKIFTIAYGGDADA
                     DLLLRIANHTNGKTFVGDPNSIETIYNAISAEQ"
     gene            complement(2195..2962)
                     /locus_tag="PL000_21645"
     CDS             complement(2195..2962)
                     /locus_tag="PL000_21645"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC91544.1"
                     /translation="MSTDYAARASIGMLKRIPAGVFRMGSKFHPREYPAKLTQVSDFQ
                     IAQTPVTVSQYEPFIDSGMIKEKKWWSDEGWEWFNGRGEGWGRKNREIPDGWKAQVNK
                     SFHPVTGVTFFETEAYCAWLGEMKGKAIRLPTEEEWEYAARGDDSRPFPWGEDFRATF
                     ANTLESEHSSTVEAASMAHDSSSFGVMDMCGNVQQWTSSVYTPLKDEVTPPGALQVAR
                     GGSFNDTLFGSRTSYRRGYPRGFFYPYLGFRIVVNNL"
BASE COUNT          617 a          843 c          812 g          783 t
ORIGIN      
        1 cggccttcaa taattcgtat ccgcgcccgt agagttcgat cttttcgttg cgttcctgtt
       61 gattcatgat ttggtttctt ccttttcagg ggtggtcttc ttcaatgcct catcccgttc
      121 acggatcgct cccagcaggc gggcttgctc tgcttttagc tcacggttct cctgttcagc
      181 gcgggccagc cgcatggacc ctgtgcggcc caatgcgcgc ccgcccgcca gatccacaag
      241 caggacgatc aggtaaccga tgatccccac aaggacgatc caccacaaac cgacgatgaa
      301 tgtggtgaca atgctcatga tcaggatgag ccagccgtat gggtcgagca ggaaccaata
      361 tggacggggt tgctgcatgt cttattgctc cgcggaaatg gcgttgtaaa tggtctcaat
      421 gctgttcggg tcgccgacaa aggtctttcc gttggtgtgg ttggcaatgc gtaacagaag
      481 gtcggcgtcg gcatctccgc cgtaggcgat ggtgaagatc ttcgtgccat ccacgctttc
      541 tccacttggc agacagttga acatttgatt ctgcgagcgg gtgctggagg tatcctgtcc
      601 atcggatagt agcacaatac cgtacaggcg tttctcgttg ttcgcgtcgt gttcagcctt
      661 gacttcgttc actttgtcga tggctgtgca taccccatca tagagcggtg tgttgccgtt
      721 cgcatacaga ccattgagct tggtcgtcag gtcttcgctg atatcgcctg cgcgcccgcc
      781 tcccaatgag tagactgtcc catcgccccc gccaaaaaca atgaggaaga tctcatcgtt
      841 cgggtcgagc ctgctgatga agtttgccga gctggagacc gcattggcga tcttctcacc
      901 ttccatgctg cctgagatgt cgagcaacag cacaatggtg gatttcttct ttgtttggtg
      961 gaagacgtct ttcactgccg aagacacatc cgcgcttggg ctttgcagag caggcacggt
     1021 gtccatggtg atgcgggggt cggtcccatc atcgagggca agcggcgcat gcaatggaat
     1081 cgtcgtgtcg atcgggcgca ggtagtagtc aattgccatt tcctgctgct gtttatcgag
     1141 caggtaatcg cggaaggtgg ctgccgcctc tttttgttcg tcacttaccc attccccgtt
     1201 aaggatgcag tacggttgtt cgctccagaa cgtgcctttg gacggaaaga tgaagaccaa
     1261 cggaaagcgc atggtggatg cgaattcctt gtttgtcttg agcgtttccg cttcgctggt
     1321 ggtgaccgca tgcaggtact caggtccgcg ctgggcgagg atctgcatca atgggcggct
     1381 ttgcaaacca tagtggtagg tgttctgttc cacgcctgtg aatgcctcaa cgaccgcatc
     1441 tgaatacacc tgatctgcgg tgagcccttc ggtgatcccg agcgtggaat aggtcagggc
     1501 ggtcatgctc aacagaccga cattggaata atcgggatgt gtgtggccga acttgaattg
     1561 tccccactca ggatgtccaa gcgtttccca tccctttggg tctgctgaaa gcgcggcgat
     1621 atcgtcccag ctgatggggg tatcgggcca gccgagcgcc tgtgccatgg gtttccacat
     1681 cgcaaagccg atcggggcca gcacagtctg tgcacattga tctgggatca gcacctgccc
     1741 ggtgcggtca cgccagaccg tgttcgcttc atccacccat gattgatcgc ccgggctcca
     1801 taccgtcggc tgactctttc catttaggat atctttctgc gatccacctg aagtgacatg
     1861 tttcactgta acgaagatca ggttgccgtc cgcggtttta tgctgttccg cgttgaattt
     1921 ttcaacgaca gcgttcatcc aatcttcttt cgtattggat gatgagatct cgatcgcaac
     1981 cgacccgggc gggggagcgg ccgttgcatt cgttgtgtct gtcactgcgg ttgcgcctgc
     2041 aggcggaaca gtattgcttt gcaacatctg tttgagggtg gggatcataa acgcgccgag
     2101 aatgatacaa ccgaataaca gcacggcaat aagtaccacg atcgcgcttc ctgaaacttt
     2161 tggtttttct gagaagtcca tgcttcctcc aaatttataa gttgttcaca acgatgcgaa
     2221 accccaggta cggataaaag aagccgcgcg gatatccgcg gcggtaggag gttcgcgagc
     2281 caaagagggt atcgttgaat gaacctccac gcgccacttg caatgctcct ggcggggtaa
     2341 cctcgtcttt caacggtgtg taaaccgatg atgtccactg ctggacattt ccgcacatat
     2401 ccatcacgcc aaaggaactt gaatcgtgtg ccatgcttgc ggcttccacg gtggaactgt
     2461 gttcactttc aagggtgttt gcaaaagtgg cacgaaaatc ctcgccccat gggaagggac
     2521 gtgaatcatc accacgtgcg gcatattccc attcctcttc ggtcggcagg cggattgcct
     2581 ttcccttcat ttcgcccaac caggcacaat aggcttccgt ttcaaaaaaa gtgacccccg
     2641 tcaccgggtg aaaggattta tttacctgcg ctttccatcc atctggtatt tccctgttct
     2701 tgcgccccca gccttcgccg cggccgttga accactccca gccttcatcg ctccaccatt
     2761 ttttttcctt gatcataccg ctgtcaatga atggttcata ctgactcaca gttactggag
     2821 tctgggcgat ttgaaaatcg gatacttgcg taagttttgc tggatactca cgcggatgga
     2881 acttacttcc catgcggaac actcccgctg ggattcgttt caacattcca atggaagccc
     2941 ttgccgcata atcggtagac atcgccaatc accaatgacc aatcgctgct ttactcaatc
     3001 tccactgcca tggctaccgc ttcgccgccg ccgaggcaga gcgatgcgac tccgc
//
LOCUS       DAOQLC010000278         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18425_length_3009_cov_19.228165, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000278 DAOQLC010000000
VERSION     DAOQLC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18425_length_3009_cov_19.228165"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PL000_21650"
     CDS             complement(<1..183)
                     /locus_tag="PL000_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91545.1"
                     /translation="MQKSDSMAPEYELDGHASSLIYAFKRGAKHLTDIFAYSKWTLPQ
                     KGSLLVDHVIISNCPST"
     gene            234..1703
                     /gene="istA"
                     /locus_tag="PL000_21655"
     CDS             234..1703
                     /gene="istA"
                     /locus_tag="PL000_21655"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNC91546.1"
                     /translation="MSCTKEQVNLLFKYYKRIPLRQAAAKAGMSINTARKYVRGAPPG
                     GQSTPRNYLTRPDRFAGHWEEICGLLKNDDGLQAKTILEYLIDRYPGVFNESHGRTLR
                     RRIERWRAQHGTGKEVYFEQFLSPGKQSQSDWTDCRELGVTIGGEPFPHLLYHFILPY
                     SGWQSVNIAFSETFESLTSGYMKAVSELGAVAPDHRTDNLTAAVKDSRDPQGFNERWV
                     GFLRHYNASPSKNSPGKSNENGSVESRHHGLKTALDQTLRLRGSRAFRDIAHYRTFLE
                     GLVRRLNDRRESLVLEELQVLQPLPPRHWNNPDECTASVTPFSVVTIDGSIYSVPSRL
                     IGKVLKALVYPDTIKLFFGTVLVQEMPHLRKGERKIDYRHVISWLVRKPGAFAAWVFR
                     DEFFPSVVFRRCYDRLLEHHSSQRQADKEYLNILNRAALDGEIKVETALCQILLAGLI
                     PNEELVKERAATKPASPPDITVLAPDLSIYNGLMQSRRL"
     gene            1714..2514
                     /gene="istB"
                     /locus_tag="PL000_21660"
     CDS             1714..2514
                     /gene="istB"
                     /locus_tag="PL000_21660"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HNC91547.1"
                     /translation="MKPPKQTQELEHLLRLFHLSSFRAHYLDFARKAEREKLNHVEYL
                     HQLAIAEEQDRSKRKIARLLKNARLPHSKLLADYQFERQPLLSRTLINELIAGDCLDA
                     CENLIIFGEPGTGKSHLSVGLGREWCLQGRSVFYTSAAALVQELLRAKRELSLNQFIK
                     TLDGFQALIIDDLSYIPQTRDETDVLFVLLSERYERRSVVVTSNLAFSDWHQVFKDPM
                     TTAAAIDRLVHHSEILELKGPSFRADEALKKTTKRTKQAPETDASSKE"
BASE COUNT          727 a          885 c          724 g          673 t
ORIGIN      
        1 tgttgatgga caattagaaa tgatcacgtg gtcaactaaa agtgatcctt tttgggggag
       61 tgtccatttg gagtaagcga agatatccgt aaggtgtttc gcgccacgct taaatgcata
      121 gattaggctg ctggcatggc cgtctaattc atattcaggc gccatgctgt cggacttttg
      181 catggggtct gattccacgt gacactgcgg gtgatggtca acccggagtc agcatgtctt
      241 gcacaaagga acaggtgaac ctcttgttta agtactacaa acgtattcca ctgcgacagg
      301 cagccgctaa ggctggtatg tcaatcaata ccgctcgaaa gtatgttcgt ggcgccccgc
      361 caggtggaca gagcacaccc agaaactact taacccgccc tgatcgcttt gctggtcact
      421 gggaggagat ttgcggtctt ctcaagaatg atgatggact ccaagcaaag acgatcctgg
      481 aatacctcat tgataggtat ccaggcgtct tcaatgaatc acatgggcga acactgcgcc
      541 gtcgcattga gcgttggcgt gcccagcatg gcaccggcaa ggaagtatac ttcgaacagt
      601 tcctctctcc cggcaagcag agccagtcgg actggaccga ttgcagagaa ctcggagtaa
      661 caatcggcgg tgagccattt ccacatcttc tgtatcactt catcctgccg tactccggtt
      721 ggcaatcagt caacatcgcc ttttctgaga ctttcgaatc tctcaccagc ggctacatga
      781 aagccgtcag tgagctgggc gctgtcgccc cagatcacag gactgataat ttgaccgccg
      841 ccgtcaagga tagccgcgac ccgcagggat ttaacgaacg ttgggtaggt ttcctcaggc
      901 actacaatgc cagtccttcc aagaactcgc ccggcaagag caacgaaaac ggcagcgttg
      961 aaagtcggca tcatggtctc aagactgcac ttgaccaaac cctcaggctc cgtggcagtc
     1021 gcgctttccg ggatattgcc cattacagaa cttttctcga aggccttgtg cgtcgcctga
     1081 acgaccgccg agagtcattg gtcctggaag agcttcaagt gctccaacca ctgccgccac
     1141 gccactggaa taacccggac gagtgcaccg cctctgtaac acccttcagc gttgtaacca
     1201 tcgatggatc aatctactcg gtgccaagcc gactcatcgg caaggttctg aaagccctag
     1261 tctaccccga taccatcaag ctcttcttcg gtaccgtcct cgtacaagaa atgccccacc
     1321 tgcgcaaagg tgagcgaaag atagactacc ggcacgtcat tagctggctt gttcgtaagc
     1381 cgggcgcatt tgccgcctgg gtctttcgtg acgaattctt cccatctgta gtctttagac
     1441 gctgctacga ccgtcttctt gagcaccaca gctctcaacg ccaagccgac aaggaatatc
     1501 tcaacatcct caatagagcc gctctcgatg gtgagattaa ggtcgagact gctctctgcc
     1561 aaatcttgct ggcaggtctg attcccaacg aagaactcgt taaagaacgt gccgctacta
     1621 aacccgcatc accacctgat atcactgttc tcgcccctga cctcagcatt tacaacggtc
     1681 tgatgcagtc tcgccggctc taggagttct cccatgaaac caccaaaaca gactcaagaa
     1741 ctcgagcact tgctcaggct ctttcacctt tcttcattcc gggctcacta cctggacttc
     1801 gcccgcaaag ccgaacgcga gaagcttaac catgtggagt atctccatca gctcgccatc
     1861 gctgaagaac aagaccgctc taagcgcaag atagccagac tgctcaagaa cgctagactc
     1921 ccacacagca agcttcttgc agactaccaa ttcgaacggc agcctttgct ctcccgcacc
     1981 ctcatcaacg agttgattgc cggcgactgt ctcgatgcct gcgaaaactt aatcatcttt
     2041 ggtgagcccg gtaccggcaa gtctcacctc tctgttggac tcgggagaga gtggtgcctg
     2101 caaggacgct cggtcttcta cacctcggcc gctgccctgg ttcaagagct gcttcgtgca
     2161 aaaagagaac tctccctcaa ccagttcata aagactctcg acggctttca ggctctcatc
     2221 atcgacgacc tgtcctacat accccagact cgcgatgaga cggatgttct cttcgttctc
     2281 ctttctgaac gctacgagag gcgcagtgta gttgtcacca gcaaccttgc cttctctgac
     2341 tggcaccagg ttttcaaaga tcccatgacg accgccgccg cgattgacag acttgtacat
     2401 cattccgaaa tcctcgaact gaagggtccg agcttccgcg cagatgaggc cctcaaaaag
     2461 actaccaaac gcacaaaaca agcgccggaa accgatgcct catccaagga gtaaactaaa
     2521 atgttcaaaa tactcatcag tattcaatgc gaagaatgcg gtgattgcca ctctctcagt
     2581 gtcttgtcta aggagataga tccgatctcc tggcggactg atgcaagtac cctgcaagct
     2641 gatgctgaag gcatcggttg ggacttcttc cagaaagccc ggtgccccca ctgtagcggt
     2701 gcgtccggtg agctcttccg cgagcatccc ggagactact acggtgaact tgtctacagt
     2761 gccaccgaca cgcccttgag ctagccgggc taccactggc aaaggctgag gttccgctgg
     2821 gcttgcaccg cccagcggct caccaccctt tcgtccgcag cattcgctgc gtgcctcaag
     2881 ggcggtctca actcgccctt gaacagagca ccagggaccg gtggacccta ctgaaagtcc
     2941 gaggcagaat acctgagtta atgatcaatt ttaattgacc acgggcacaa aagtagttga
     3001 ccgcagaca
//
LOCUS       DAOQLC010000279         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18535_length_2998_cov_20.501189, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000279 DAOQLC010000000
VERSION     DAOQLC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18535_length_2998_cov_20.501189"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1388)
                     /locus_tag="PL000_21665"
     CDS             complement(<1..>1388)
                     /locus_tag="PL000_21665"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNC91548.1"
                     /translation="QRFRVQTTVDYAKKESQALLLNPDLYISVVVPRLESINAMNVPP
                     SIKDRLREDVSSHFSETVVRGVGNKNPGEALRMLDAGEFNKLPGFNLYGDKLDRLRSH
                     LLAASNQIRSEATTQLRIALEDHLADYSVGKTTEPLPAGYNLTQVKDLLGPAGYKRYQ
                     DQVEIMSREKIIAREIRHGDLTSTVERLKTEQVSQKALVPYTETITKASRDTGVDAGV
                     LAAQIMAESNGNPNARNDKTSTGQPSLGISQFQPDTAKQYGVDTTDPHSSIAGQARYM
                     KDLLNQFGGDYSKALAAYNWGQGRVAKAVEKYGSDWMSNIPSETQAYVKKILSSSPNG
                     VAGAKIAGATADMLQAEAKLRASDPAAAAERNAIELDPTNYPGQPIAARVATRLGIQQ
                     NQFKLPEFQQRLLTKDEAQGYVKRIKDAGTLDEAQQIMSQIQQEFVGQNQTPQNKRLY
                     AKFMGELYANGL"
     assembly_gap    1389..1488
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1489..1751)
                     /locus_tag="PL000_21670"
     CDS             complement(<1489..1751)
                     /locus_tag="PL000_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91549.1"
                     /translation="MAVATSTAMLASTAASAFSNIWAGQQGKAAAEFNAKQAELDADL
                     AKQQAAFEERNFRAGVDRLLGQQRAGYAKAGVQMTGSALDVAQ"
     gene            complement(1761..>2998)
                     /locus_tag="PL000_21675"
     CDS             complement(1761..>2998)
                     /locus_tag="PL000_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91550.1"
                     /translation="WFANTTQQPQTVWATRSGDFNNFAPSDAAGKVLDDSGLNYTLST
                     DDVNSIRWMVPGKVLVILTDSGEFTVSASSLYEAITPTNVRVIRETARGAANVKPVLV
                     DKNLLFWQRARRKLREYFYDFNIDGFRSNDVTILSEHITLGGMTSMDYQQEPHSIVWS
                     ARADGQLIGFTYNKEQEVLGWHRHILGGTNAQVKHVACIPGADNDELWAVTTRTIDGG
                     TKQYIERLDPEFYPSSSEDKDGAFFVDCGLSYSGTPITLVSGLDHLKGETVSILGDGS
                     VRAPKVVNSSGQITLDRPASVIHIGLAYTAKLRSIRYEAGGNEGTAQTKTGRVQRLGL
                     RLLNTLGLKFGPSEDKLQEIQFRMGFHKMDQSPPLFTGDQVVNFPGDYDRSRQVTLVA
                     DQPYPCTITGLVPWMVVYE"
BASE COUNT          641 a          809 c          739 g          709 t
ORIGIN      
        1 tcaaggccat tcgcatacaa ctcccccatg aacttagcat acaggcgctt gttctgaggc
       61 gtctggttct gaccaacaaa ctcctgctgg atttggctca taatctgctg ggcctcgtca
      121 agggtcccag catccttaat ccgctttacg taaccctgag cttcatcctt cgtaagcagt
      181 cgctgttgaa actccggaag cttgaactga ttttgctgaa taccaagacg agtagcaaca
      241 cgggcagcaa taggttgccc aggatagttg gttggatcaa gttcaatggc atttcgttca
      301 gcagctgcag ctgggtcact tgcacgtagt ttagcttcag cttgcaacat gtcagcagtg
      361 gctccagcaa tttttgcccc ggccactcca tttgggcttg acgataaaat ttttttgacg
      421 taggcttgcg tttcagaagg aatgttactc atccagtctg agccgtactt ctcaacagcc
      481 tttgctacac ggccctgccc ccagttgtac gcagccaggg cctttgaata atctccaccg
      541 aactggttga gcagatcctt catgtagcgg gcttgacctg caatactcga atgcggatca
      601 gttgtatcaa ccccgtattg tttggccgtg tccggttgga actggctgat gcctagcgac
      661 ggctgcccag tcgacgtctt atcattgcgg gcattcgggt taccgttaga ctcggccatg
      721 atctgcgcgg caagtacgcc ggcgtcaacg cctgtgtcgc gtgaagcttt cgtaatggtc
      781 tcggtgtagg gtacaagggc tttttgggag acctgttcag tcttgagccg ttcgaccgtg
      841 gatgtaagat ccccatgccg aatttctctg gcaatgatct tctcacggct catgatttcg
      901 acctgatcct gataccgctt gtagcctgcc gggccgagca agtcctttac ctgcgtcagg
      961 ttgtagccgg ctggcaacgg ttctgtggtc ttacccacgc tgtagtcggc caggtggtct
     1021 tccagcgcga tgcgtagttg cgtggtggcc tcagaccgaa tctgattcga tgctgccagc
     1081 aggtgtgaac gaagacgatc gagcttatct ccgtagagat tgaaccccgg tagtttgttg
     1141 aactcaccag cgtcaagcat ccgaagagcc tcgcctggat ttttattgcc gacgccccgc
     1201 acgacagttt cagagaagtg cgaagacacg tcctcacgca agcggtcttt tatgcttggc
     1261 ggaacattca tggcgttgat ggactcaaga cgcggtacca cgactgatat atacaggtca
     1321 gggttcagca gcaaggcctg agattccttc ttcgcgtagt caacagtcgt ctgtacacgg
     1381 aagcgttgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ttgcgcgacg
     1501 tccagcgctg agccggtcat ctggacaccc gccttagcgt aacctgcgcg ttgttgacca
     1561 agcaatcgat caacacccgc tctgaaatta cgttcctcaa acgcagcctg ctgcttggcc
     1621 agatcggcgt ctagctctgc ttgcttggcg ttgaactctg ccgcagcctt accttgctgc
     1681 ccagcccaga tgtttgagaa cgctgatgcc gccgtagagg ctagcatagc cgttgacgta
     1741 gcgacggcca tgtgccctcc ttattcgtag accaccatcc acggtactag gccagtaatc
     1801 gtgcatggat acggttgatc tgctacgagt gtaacttgac ggctacgatc gtagtcccct
     1861 gggaaattca ctacctgatc cccagtaaac agcggcggag actgatccat cttatgaaag
     1921 cccatccgga actgaatctc ctgaagcttg tcctcagacg gaccgaactt caaccctagc
     1981 gtattgagca gccgcaaacc caggcgctgg acacgcccag tttttgtctg agccgtaccc
     2041 tcattaccac cggcctcgta acgaatagac cgcaacttcg ccgtataggc tagaccaatg
     2101 tggataacgg acgcaggacg atcaagcgtg atttgaccag aactattaac caccttggga
     2161 gcgcgcaccg agccatcacc gaggatggat accgtttccc ctttgagatg gtctagaccg
     2221 gaaacaaggg ttattggggt cccactataa gagaggccac aatccacaaa gaacgcacca
     2281 tccttatcct cggatgaact cggatagaac tcagggtcta gtctctcaat atactgcttg
     2341 gtcccgccgt caatggtcct ggtggtaaca gcccagagtt catcgttatc ggctcctggt
     2401 atacacgcta cgtgttttac ctgcgcgttg gtgccaccga ggatgtggcg gtgccagcca
     2461 aggacttcct gctccttgtt atacgtgaag ccgatcaact ggccgtcagc tcgagcgctc
     2521 cacacaatgg agtgcggctc ttgctggtag tccatgctgg tcattccacc cagcgtaata
     2581 tgctcagaaa gaatcgtaac atcgttagat cggaagccat caatgttaaa atcataaaag
     2641 tattcgcgaa gcttccgacg cgccctctgc cagaagagca gattcttatc caccagtaca
     2701 ggcttaacat tagcagctcc tcgcgcagtt tcacgaatga cacgcacatt cgttggcgta
     2761 atcgcctcat acagcgaact ggccgagact gtaaattcgc ctgaatccgt caggatcacc
     2821 aggacttttc ctggaaccat ccagcgaata gagttcacat catcggtgct cagtgtgtag
     2881 ttgagtcctg aatcgtccag caccttgcct gccgcatctg acggggcaaa gttgttgaag
     2941 tcgccagaac gcgtggccca gaccgtttgt ggctgctgcg tggtattcgc gaaccaca
//
LOCUS       DAOQLC010000280         2982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18711_length_2982_cov_25.259310, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000280 DAOQLC010000000
VERSION     DAOQLC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18711_length_2982_cov_25.259310"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..108)
                     /locus_tag="PL000_21680"
     tRNA            complement(35..108)
                     /locus_tag="PL000_21680"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Val,seq:tac)
     gene            235..657
                     /locus_tag="PL000_21685"
     CDS             235..657
                     /locus_tag="PL000_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91551.1"
                     /translation="MTESKPLNWTPTAEVGYSVIRRGDGGMTLTFTDLSDATMTHWHG
                     FALEHLMGSDRRTRNLYDLRAVKEIPEKAVRMAVDANSDPSARNIRVAVVVADATVAD
                     AIRKVAALALPEAASAMKIFTDINEAEAWLARPLDQIP"
     gene            704..1618
                     /gene="folP"
                     /locus_tag="PL000_21690"
     CDS             704..1618
                     /gene="folP"
                     /locus_tag="PL000_21690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC91552.1"
                     /translation="MKSSSLQIGSFTFNWGSRTYVMGILNVTPDSFSGDGIIARGDAI
                     HTAVEQARGFLASGADILDVGGESTRPGSAPVNADEEMERVIPIVQALKQNFPDALIS
                     IDTYKAQVAEEAVRAGARIINDVWGLRADDGLAAVAAKYNVPVILMHNRSNPASVEVR
                     EKLGGAYVGSEYQNLLEDVKRELLECVALAKKAQIAESHIVLDPGVGFGKTREHNLEL
                     IDRLGEIRSLGYPVLLGTSRKSFIGFTLDLPADQRVEGTAATVAVGITRGADIIRVHD
                     VKEMARVAKMTDAIVRKPFTINERDKRG"
     gene            1653..2981
                     /locus_tag="PL000_21695"
     CDS             1653..2981
                     /locus_tag="PL000_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91553.1"
                     /translation="MDDYGKELLRTGIIEAKTGNRDSARRYLDRAIYMSSSFDVLAEA
                     WFWMSEVVDDLAEKRKALENCLAHEMHHARARRSLAILDGKLKADEVVNPDRLPVVTD
                     ETRTSDAQRFMCPKCGGRMSFSPDGQGLVCEYCTRNQTLDSGTASEKDFIVAMATARG
                     HGKPLQEQVFHCKGCGAEFILPPKQISASCAYCNSPHVVSLEKTKDLLAPDGIIPHAF
                     NQKQAIKLLINWVEGNQIKPEKQVELPRGLYLPLWTFDIGGSVEYTYEMEDVNRRNRN
                     RGLQLGEMEPVRITSDYPVMEDDLPLPASKKPSAVFLKMIPTFDMKSIQPYDPRFLVD
                     WPAEIYDVPMADASLDARSRVFAKFKRNLPSMTNHANIVRTSSANLLVESFKLNLLPV
                     WMTELPFNGREHLVLINGQNGVVHSDFAEKVEEEDEKGGLLGFLADLLDD"
BASE COUNT          699 a          700 c          872 g          711 t
ORIGIN      
        1 aaataaaaat accccaaggt gacggggtat tttgtgggcg ctgagggact cgaacccccg
       61 accccttcgg tgtaaacgaa gtgctctaac caactgagct aagcgcccgg gtgacccgca
      121 ttatagcatg tgattcgaaa aatgaaagat ttcgatttcc ctacaaacat gaaatttgcc
      181 gacccgtgat atttgcctgc gttataatca aattaatcaa aacaggagag acccatgact
      241 gaatcaaaac cactgaattg gacgcccacg gctgaagtcg gttattccgt gatccgccgc
      301 ggcgatggcg gcatgaccct gaccttcacc gatctttcag acgccaccat gacccattgg
      361 catggattcg cgctggagca cttgatgggt tcagaccgcc gcacacgcaa cctgtatgac
      421 ctgcgcgctg tgaaggagat ccctgaaaaa gcggtgcgca tggccgtgga tgccaatagc
      481 gatccctctg cgcgaaatat ccgtgtggcg gtggtggttg cagatgcgac tgtggcagat
      541 gccattcgca aagtggcagc actagctctt ccagaagcgg cttcggcgat gaagatattc
      601 accgatatta atgaggcgga agcctggctt gcccgtccgc ttgaccagat accgtaaaca
      661 caaagcctga tgggtttcga agaccttcag gctttctttc tttatgaaat catcttcctt
      721 gcaaatcggg agttttacat tcaactgggg ttcccgcacc tacgtgatgg gaatcttgaa
      781 tgtgactccc gattcgtttt ccggtgatgg catcattgcc agaggagatg ccatccatac
      841 cgctgtggag caggcgcggg ggtttctcgc ttcaggggcg gatattctgg atgtaggcgg
      901 cgagtcgacg cggcctggtt ctgctccagt caatgcagat gaagagatgg agcgggtgat
      961 ccccattgtg caggcgttga aacagaattt ccctgatgcg ttgatttcaa ttgacaccta
     1021 caaggcgcaa gttgcggaag aggcggtccg ggctggggcg cggatcatca atgacgtgtg
     1081 ggggctgcgt gccgatgacg ggctggctgc tgtggctgcg aaatacaacg tgcctgttat
     1141 tttgatgcac aaccgcagta acccagccag tgtcgaagtc cgcgagaagt tggggggggc
     1201 gtatgtgggt tcggaatatc agaatctgct tgaagatgta aaaagggaat tgcttgaatg
     1261 tgtggctttg gcgaagaagg cccaaattgc tgagtcgcat attgttttgg atccaggcgt
     1321 cggctttgga aagacgcgtg agcataatct cgagttgatc gatcggttgg gggagatccg
     1381 ctcgttgggg tatcccgttt tgttggggac atcccgcaaa tcgtttattg gttttacgct
     1441 tgacctgccc gccgatcagc gtgtggaagg tacggcggcg acggtggcag tgggcatcac
     1501 gcgcggtgct gacattattc gtgtgcatga tgtgaaagag atggcgcgtg tggcgaagat
     1561 gacggatgcg atcgtgcgaa aaccattcac cataaacgaa agggataagc gcggataaat
     1621 ttttatctgt gttcatctgc ggttaaaaaa acatggacga ttacggcaag gaacttcttc
     1681 gcacggggat cattgaagcg aaaacaggca atcgcgattc agcccgccgc tatttggatc
     1741 gggccattta catgtcgtct tcatttgatg tgctggccga ggcatggttt tggatgagcg
     1801 aggttgtgga tgacctcgct gaaaagcgca aggcattgga gaattgcctt gcgcatgaaa
     1861 tgcaccatgc acgtgcgcgg cgttcgctgg cgatccttga tgggaaactg aaggcggacg
     1921 aagttgtaaa ccccgaccga ttgcctgtcg tgaccgatga aacacggact tcagacgcgc
     1981 agcggtttat gtgccccaag tgcggcggac ggatgtcttt ttcacctgat gggcagggat
     2041 tggtctgtga atattgcaca cgcaaccaga ccctggattc agggacggcc agtgaaaagg
     2101 attttattgt tgccatggcc actgcacgcg gacacggcaa acccttgcag gaacaggtct
     2161 ttcattgcaa aggctgcggc gcggagttta ttttgccgcc gaaacaaatt tctgcctcat
     2221 gtgcgtattg caattcgccg catgtggtga gcctggaaaa gacaaaagac ctgcttgcgc
     2281 ccgatgggat cattccgcat gcgttcaacc agaagcaggc cattaaattg ctcatcaatt
     2341 gggtggaggg gaatcagatc aaacctgaaa agcaggtgga acttccgcgc gggttgtacc
     2401 ttccgctgtg gaccttcgat attggcggtt cggtggaata cacctatgaa atggaagatg
     2461 tgaacaggcg aaacaggaat cgcgggttgc agctcggtga aatggagcct gtgcgcatca
     2521 ccagtgatta cccggtgatg gaagatgacc tgccattgcc cgcttcgaaa aagccgtcgg
     2581 cggtcttttt gaagatgatc cccacttttg atatgaaatc catccagccg tatgatccgc
     2641 gctttcttgt ggattggcct gccgaaattt acgatgtgcc catggctgac gcttcactgg
     2701 atgcacgcag ccgtgtgttt gcgaaattca agcgcaacct tccgagcatg accaaccatg
     2761 cgaatatcgt gcggacctct tctgccaatc ttttggtcga atcttttaaa ctcaacctgc
     2821 tcccggtgtg gatgacggaa ctgccgttca acgggcgcga acatctggtg ctcatcaacg
     2881 gccagaacgg tgtggttcat agcgacttcg ccgaaaaagt tgaagaggaa gatgaaaagg
     2941 gtgggctgtt gggctttctt gcggatctgt tggatgattg at
//
LOCUS       DAOQLC010000281         2978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18763_length_2978_cov_19.731782, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000281 DAOQLC010000000
VERSION     DAOQLC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18763_length_2978_cov_19.731782"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1107
                     /locus_tag="PL000_21700"
     CDS             178..1107
                     /locus_tag="PL000_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91554.1"
                     /translation="MKARLLLNEIQQQRRLSDQVIKIGLDALMDIYYPGLAPDAIHFA
                     EIGSYGRGTNNDPEPDIDVMYLGIPNDPEQGFFNWVGIGTSKMAQDPAGLTALSQVQE
                     YDPKLAQAIAATIKRLEDHFGCDGQASFNFVRSWPEFPGVVFNLSAPIPGYGQLGFDI
                     NLYHPKEYFGVEHSKRFVHYVERVRAEMGEEIAAKLILDIRQVKQAAKDFARDPKTGQ
                     IRKAKKVWGITCEALFTYCYPPPSRDTLFREIKSMDAGSFPSVLSGTDYTYPIQVIDE
                     NLSLKKVLDVGWETGLFHERNWETLVSALEPYD"
     gene            1212..>1704
                     /locus_tag="PL000_21705"
     CDS             1212..>1704
                     /locus_tag="PL000_21705"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91555.1"
                     /translation="MLTISQVNSEVEIAAVQELMREYATWAFSIVPGSEDSPAWAGFE
                     AELATLPGVYAPPEGRLLLAVDDGHPAGCVCLKRHDDNVSELKRLFVRPSFRGKNIGR
                     HLVKMLINEARQSKYQRIVLDSHISMKKAHAIYLEAGFRLVNAPDDFPEDIKPVVVFM
                     ECDL"
     assembly_gap    1705..1804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1812..2801)
                     /locus_tag="PL000_21710"
     CDS             complement(1812..2801)
                     /locus_tag="PL000_21710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNC91556.1"
                     /translation="MQNPPKPTSSPQGKKLLDQYREHIRLKQYSPRTEKTYIEWVRQY
                     ILYHNKRHPKEMGAPEIKQFITHLVVDRKASASTQNQVISAIIFLYRNVLHLELDQTD
                     LGFIRPKRGKRVPTVLSKDEAKAIIQNLNQPYKLMVQIMYGSGLRLMECLRLRIKDID
                     FENRRILVYDGKGGDDRQTPLPDSITPALREHLAKTRAIHQKDLAHGRGSVHMPYALA
                     KKFPNAEKSWIWQYVFPATTFLTDKEDGITRRHHIHETALQRAIKQAATLAKIEKRVT
                     PHTFRHSFATHLLQNGYDIRTVQELLGHKDVKTTMIYTHVLQRGANAVKSPLD"
BASE COUNT          602 a          528 c          912 g          836 t
ORIGIN      
        1 gagcaaggca atttcgtcca atctgcggct ggctggtttc tacgttttgg catttttgtt
       61 ttgccaggcc tgcaagattt tttacgaatg caggattgtt tgggacgtcg ttacgattta
      121 gtttgcatga tgaaataacc gaagataatg tttagtggtt ttatgaaatg agtgcagatg
      181 aaagccagat tattgctcaa tgaaatccaa cagcagcgca ggttaagcga ccaagtgata
      241 aaaataggcc tggatgcttt gatggatatt tactatccgg ggcttgctcc ggacgcgatc
      301 cattttgctg aaattggctc gtatggccgc ggtacgaaca atgaccctga gccagatata
      361 gatgtgatgt atttgggaat tcccaatgac cctgaacaag ggtttttcaa ctgggtcggc
      421 attggcactt cgaaaatggc ccaagaccct gctggtctca ctgcgctgtc acaggttcag
      481 gaatacgacc cgaaactggc ccaagcgatc gcggcaacga tcaaacgcct ggaagatcat
      541 tttggctgtg atgggcaggc ttccttcaac ttcgttcgtt cctggcctga attcccaggt
      601 gtggttttca atctttctgc gcccattcca ggttacgggc agcttggatt cgatatcaat
      661 ctttatcatc cgaaagaata ttttggcgtt gaacattcaa aacgttttgt acattatgtg
      721 gaacgtgtga gagcagagat gggggaagaa attgcggcaa agttaatcct ggatattcgt
      781 caggttaagc aggctgcaaa ggattttgcg agagacccga aaacagggca aatccgtaaa
      841 gccaaaaagg tgtggggcat cacctgcgag gcgcttttta cctattgcta ccctccccca
      901 tcaagggata cattgtttcg ggagatcaag agcatggatg caggttcatt tccttccgtg
      961 ctttcgggca ctgactatac ttatccgatc caagtcattg atgagaattt gtctctgaaa
     1021 aaggtgttgg atgttgggtg ggaaacgggt ctttttcacg aaagaaattg ggagacgtta
     1081 gtgagtgctt tggagccgta tgattgaacg ctgcccaccc cagtatgcgt ttgaagctgg
     1141 ggacgacggg ggcaaaccgt ttgacgatta tcagtaaact catattagat gctgccaaag
     1201 gaagcccgac catgttaact atttctcaag tcaattcaga agttgaaatt gctgccgtac
     1261 aggaactcat gcgtgagtat gcgacatggg ctttcagtat cgttccgggc agtgaggatt
     1321 cgccagcctg ggcagggttc gaggcagaat tggcgacatt gcccggggtt tatgcgccgc
     1381 ctgaggggcg tctgctatta gctgtggatg atgggcatcc tgcagggtgt gtttgcctca
     1441 aaaggcatga tgacaatgtc agtgagctca aacggctttt tgtgcgtccc agttttcggg
     1501 ggaaaaatat cgggcggcat ttagtcaaaa tgctgatcaa tgaggcgcgt caatccaagt
     1561 atcaaaggat cgtgctggat agccatatct cgatgaaaaa ggcgcatgct atttatctgg
     1621 aggctggttt tagattggtg aatgctcctg atgattttcc tgaggatatc aagccagttg
     1681 tggtgtttat ggagtgtgat ctatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnggggtg gttagtctag cggggatttg acggcgttgg cgccgcgttg gaggacgtgg
     1861 gtgtagatca tggtggtttt gacgtctttg tggccgagga gttcctggac ggttcggatg
     1921 tcgtagccgt tttggaggag gtgggtggcg aaggagtggc ggaaggtgtg gggggtgacg
     1981 cgtttttcta ttttggcgag ggttgcggct tgtttgatgg ctctttggag tgcggtttca
     2041 tgaatgtggt ggcgccgtgt gattccgtct tctttgtctg tgaggaaggt ggtggcgggg
     2101 aatacgtatt gccagatcca tgatttttcg gcgttgggga attttttggc gagggcgtag
     2161 ggcatgtgga ctgagccgcg gccgtgggcg aggtcttttt gatgtatggc gcgggtcttt
     2221 gcgaggtgtt cgcggagggc gggggttatg ctgtcgggta ggggtgtttg tcggtcgtcg
     2281 cccccttttc cgtcgtagac aaggatgcgt cggttttcga agtcgatgtc ttttatgcgg
     2341 aggcgcagac attccatgag gcgtagtcct gagccgtaca tgatctgtac catgagtttg
     2401 tagggttggt ttaggttttg gatgatggct ttggcttcgt ctttggagag gacggtgggg
     2461 acgcgtttgc cgcgtttggg gcggatgaag ccgaggtcgg tttggtcgag ttcgaggtgg
     2521 aggacgttgc ggtagagaaa gatgatggcg ctgatgacct ggttttgggt ggaggcggag
     2581 gctttgcggt ctacgacgag gtgggtgatg aattgtttga tttcgggggc gcccatttct
     2641 ttggggtggc gtttgttgtg gtagaggatg tattgacgga cccattcgat gtaggttttt
     2701 tcggtgcgcg gggagtattg tttgaggcgg atgtgttcgc ggtattggtc gagtagcttt
     2761 ttcccctgtg gggatgatgt gggtttgggt gggttttgca ttttcttgcc cctttctgct
     2821 ggtgattgta taataacttt atgtaatcta caaggtgtta tacggcgaaa ttgctgttat
     2881 gtactccatt tggggtgtta tacagcgcgc tgtataaaca gtagttgggc ggctggcgta
     2941 aaggataggc ggctatgaaa ttcaaaaata tcatcacg
//
LOCUS       DAOQLC010000282         2976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18789_length_2976_cov_19.042451, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000282 DAOQLC010000000
VERSION     DAOQLC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18789_length_2976_cov_19.042451"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..664
                     /locus_tag="PL000_21715"
     CDS             62..664
                     /locus_tag="PL000_21715"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC91557.1"
                     /translation="MPKKKTYHHGDLKNALIKAGVEILAKDGVSGLSLRKVALKAGVS
                     HAAPYAHFADKQALIAAISTEGFRQLYERVSGVAEKYQSQPEKQLTEAAWAYVQFAMD
                     DRDRFKVMFSGVLEKEKEYSDFVVESQRNFQLVKSIVEANQASGRLRGGDSALVALSA
                     WGIIHGFLMLLLEGQISHAVLEKMSLRELVEFQLEQIKVK"
     gene            complement(704..964)
                     /locus_tag="PL000_21720"
     CDS             complement(704..964)
                     /locus_tag="PL000_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNC91558.1"
                     /translation="MNFLVWIIFGAIAGWVASIIMKRNKQMGALANIIVGVLGAFIGG
                     YIMDFFGAEGVTGFNIPSLLVAILGAVLLLWIGNLLTGRRRR"
     gene            complement(1007..1750)
                     /locus_tag="PL000_21725"
     CDS             complement(1007..1750)
                     /locus_tag="PL000_21725"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC91559.1"
                     /translation="MQLYFIRHGQSINNAKWEEPGYVESPDPHLTEIGQEQAQVLAAY
                     LKANHPITEKEARNNQNQFGYGFTHIYTSLMERAANTAAPTARALGVPFTAWVDIYEE
                     GGIYAREKENLFQGLPGKPRSYFEKNHPALNLPESYNESGWWNRPYESEEERQPRADK
                     VLKELIHRHGDRAGQPEERIVFVSHGGFFVRLLSAMLKLPWRQGAHDMKSWFILNNCS
                     ISRFDFRDGEILINYLNRTDHLPARLITE"
     gene            complement(1779..2582)
                     /locus_tag="PL000_21730"
     CDS             complement(1779..2582)
                     /locus_tag="PL000_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91560.1"
                     /translation="MSRGKKIVIGIIGLLVLLCGAFMLFVNWANSDEGQAAMNEVSTK
                     QAIESATANAQATIQAEADAKAAATATAVMAAINERMQNMTLVHENTFEEGTPLLNEF
                     GEDLEPYYRDGIPELIIPWNGSTILPIGMNLTDFVAEVDCEANGTFCGIAYSYTTDSE
                     GKNHYFASGIGRADTFFFEDLYAKVPSFNWDHKNFAPSTSKEFLHHIRVERFGKVLRL
                     YVNGELMEERILNYPEAVSGDVGFFFGKASNDTGIQTLRMDNFRVWELP"
     gene            complement(2638..>2976)
                     /locus_tag="PL000_21735"
     CDS             complement(2638..>2976)
                     /locus_tag="PL000_21735"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HNC91561.1"
                     /translation="CYTRRMAKHKPKPIKFSWDPEKAEKNESKYGVSFDEAMTIFKDP
                     VAMYFDDLEHSQKQKREMVIGRSMPRERLLTCVFERTEGGIHIINARISTHRERQDYD
                     DNSEVEYQIQ"
BASE COUNT          645 a          649 c          868 g          814 t
ORIGIN      
        1 ttcttgacag taaataaatt atcctctaca atctttacag tgtcaagatt gagagagaac
       61 aatgccaaag aagaaaacct atcatcacgg tgacttgaag aatgcgctta tcaaagctgg
      121 ggtggagatc cttgcgaagg atggggtgag cggactgtcc ctgcggaagg tggcgttgaa
      181 ggcgggggtg agtcatgccg cgccgtatgc tcattttgcg gataagcagg cgttgatcgc
      241 ggcaatttcc acggaagggt ttcgtcaact atacgagcgg gtgagtgggg tggcggagaa
      301 atatcaaagc cagcccgaaa agcaattgac cgaggcggct tgggcgtatg tccagtttgc
      361 gatggatgac cgtgaccgat tcaaggtcat gttttcgggg gtgctggaga aggaaaagga
      421 atattccgat tttgtggtgg agtcgcagag aaattttcag ctggtgaaaa gcattgtaga
      481 ggcgaatcag gcgtcaggta ggttgagagg cggtgattcg gcgttggtgg ctctttcggc
      541 gtggggaatc atccacgggt ttttgatgtt gctgcttgag gggcagatct cgcacgcggt
      601 tttggagaag atgagcttgc gtgaactggt ggagtttcag ttggagcaga tcaaagtgaa
      661 ataaaaaaga gccgtgcaat tcgttgcacg gctctttttg aaattatctg cggcggcgac
      721 ccgtgagcag attgccgatc cagagcagca gcactgcgcc aaggatggcg acaagcaggc
      781 ttgggatgtt gaagccagtg acgccttcag cgccgaagaa atccatgatg taaccgccga
      841 tgaaagcgcc gagaacaccg acgatgatat tggcgagtgc tcccatttgt ttgttgcgct
      901 tcatgatgat gctggcaacc cagcctgcga tcgcgccgaa gatgatccaa acgagaaagt
      961 tcatttttgc ctccaataga taagttggtt tcgttttttt attttatcat tcggtaatga
     1021 ggcgtgcagg gaggtgatcg gtgcggttaa gataattgat gagaatttcg ccgtctcgaa
     1081 aatcaaagcg gctgatggag caattgttga gaatgaacca cgacttcata tcgtgcgcgc
     1141 cctgccgcca gggaagtttt agcatggcag aaagcaggcg gacaaaaaat ccgccgtggc
     1201 tgacgaagac gatcctttct tcgggttggc ctgccctgtc gccatgacga tggatcagct
     1261 ctttgaggac tttgtcggct cgcggctggc gctcttcttc cgattcgtag ggacgattcc
     1321 accagcccga ttcgttgtag ctttcaggca gattcaatgc aggatggttt ttctcgaaat
     1381 acgaacgcgg cttgccaggc aggccttgaa agagattttc cttttcacgg gcatagattc
     1441 cgccttcttc gtaaatatcc acccaggctg tgaatgggac gcccaatgcg cgggcggtgg
     1501 gtgcggcggt gttggcagcc cgttccatca ggctggtgta gatgtgggtg aaaccatacc
     1561 cgaactggtt ttgattattc cgtgcctctt tttcggtgat ggggtggttt gcttttaaat
     1621 atgcggcgag gacttgcgcc tgttcctgcc cgatctctgt cagatgtggg tcggggcttt
     1681 ccacgtaacc aggctcttcc cattttgcgt tgttgatgga ttgtccatga cggatgaagt
     1741 aaagttgcat gatggtctct gtaggtttga agtttggttt acggcagttc ccagacgcgg
     1801 aagttatcca ttcggagggt ctgaatgcct gtgtcgttgg aggctttccc gaagaaaaag
     1861 cccacatcgc cagaaacagc ttcggggtag ttgaggatgc gttcttccat caactcaccg
     1921 ttgacataca ggcggagtac ttttccaaag cgttccacgc ggatgtggtg taggaattct
     1981 ttagaggtgg aaggggcgaa gtttttatga tcccaattga aggatggcac tttggcatat
     2041 aaatcttcaa agaaaaaagt gtcggctctt ccgatgcctg aggcgaagta gtgatttttg
     2101 ccttcagagt cggttgtgta gctgtaggcg atgccgcaga acgtgccgtt tgcttcgcaa
     2161 tccacttcgg caacaaaatc ggtcaggttc attccgatgg gcaggatggt tgagccgttc
     2221 cacgggatga tgagttcagg aatgccatca cgataataag gttcgaggtc ttcgccgaat
     2281 tcatttaaga gcggggtgcc ttcttcgaaa gtgttttcat gaacgagggt catgttttgc
     2341 atccgctcgt tgatggctgc catcactgcc gtggcggtgg ctgcggcttt tgcatctgct
     2401 tcagcctgga tggtggcctg tgcattggct gtggcgcttt cgatggcttg ttttgtgctg
     2461 acctcgttca ttgccgcctg tccttcatct gaattcgccc agttgacgaa tagcatgaat
     2521 gctccgcaaa gcagaacgag caggccaatg atcccgatga cgattttctt tccgcgtgac
     2581 atgaatcttt ttctccttgg tgaatgaaaa tccccgccat ctgacgggga ttgaatttta
     2641 ctgtatttga tattccactt ccgagttgtc gtcgtaatcc tgccgctctc tgtgtgtgga
     2701 gatcctggcg ttgatgatgt ggatgccgcc ttccgttctt tcgaacacac aggtcaacag
     2761 cctttcgcgg ggcatggacc tgccaatgac catctcgcgt ttttgctttt gcgagtgttc
     2821 gaggtcgtcg aaatacatcg caacagggtc tttgaagatg gtcattgctt catcgaaact
     2881 gaccccgtat ttggattcgt tcttttccgc tttttcgggg tcccagctga atttgatcgg
     2941 tttaggtttg tgttttgcca tacggcgagt atagca
//
LOCUS       DAOQLC010000283         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18920_length_2964_cov_25.741492, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000283 DAOQLC010000000
VERSION     DAOQLC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18920_length_2964_cov_25.741492"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PL000_21740"
     CDS             complement(<1..560)
                     /locus_tag="PL000_21740"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC91562.1"
                     /translation="MEHIAEIENKYTSGVYAKQTLTIVRGQGASLFDENGVEYLDCSS
                     GHGVANLGHAHPKIAEALYKQANTLITLFETFPNDQRAMLMEKITGLIPGMDRVFFCN
                     SGTEAVEASFKFARISTGRKNIIAAMRAFHGRTFGSLSATFNKKYREGFEPLLPGVSH
                     VSYNNIEALDKAVNEETAAVILEVVQG"
     gene            complement(581..1564)
                     /locus_tag="PL000_21745"
     CDS             complement(581..1564)
                     /locus_tag="PL000_21745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC91563.1"
                     /translation="MEYVRLGSTGMKVSRICLGCMGFGDAERWLHKWVLNEESSRPII
                     KKALEMGINFFDTANVYSLGASEEILGRALRDFAKRDEVIIATKVHGKMRDTPNGGGL
                     SRKAILVEIDNSLKRLETDYVDLYQIHRWDYETPIEETMEALNDVVRAGKARYIGASA
                     MWAWQFQKALHVAEMHGWTRFISMQNHLNLIYREEEREMIPLCREEQIGLIPYSPLAS
                     GRLTRSWSAESTLRSETDQIQKSKYDSAAETDRPIIERVAELAEKHAVSRTHIALAWL
                     LQKQPVAAPIIGATKIAHLEEAAGALSVRLTQEEILYLEEPYMPHRVIGHQ"
     gene            complement(1595..>2964)
                     /locus_tag="PL000_21750"
     CDS             complement(1595..>2964)
                     /locus_tag="PL000_21750"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNC91564.1"
                     /translation="LTLEHVLNVIDKENPIGVIVQFGGQTPLNLAAGLEAAGVKILGT
                     SPHAIQLSEDREEFAKLLKELDIPQPENGIARSLEEAREVAQRIGYPVLVRPSFVLGG
                     RAMALVESEEHLAGFIQKAIEAAPGQPILIDKFMEDAFEIDVDALSDGGSVVVGAIMQ
                     HIEEAGVHSGDAACVLPPYKVSEFHLGIMREYTEQLGLALGVRGLMNVQFALKDEVVC
                     VLEVNPRASRTVPYASKATNLNLAYKAAQVMAGKTLEELGVTEEPQVDGFFIKEAVLP
                     FKKLPGSSSLLGPEMRSTGEVMGHASRFGHAFAKSQIAAGQGLPQSGAVLISVNDYDK
                     SAALKVARDLSRMGFALYATPGTADFFSKVGLLVEVVHKIDEGSTAIEDLIRGGKVQL
                     IVNTPLGTHAHSDGARIRAAAIAMNVPLLTTLSAATAAVAAIQALNKKELKYRSLQSH
                     FVESN"
BASE COUNT          693 a          849 c          683 g          739 t
ORIGIN      
        1 ccttgcacca cttccagaat cacagcagca gtttcttcgt tgacagcttt atcgagggct
       61 tcgatgttat tgtacgaaac gtgcgaaacg ccgggcaaaa gcggctcaaa gccttcacga
      121 tactttttgt tgaatgtggc tgaaagtgaa ccgaatgtcc gtccatgaaa ggcgcgcatg
      181 gcggcgatga tattttttcg tcccgtggaa atacgcgcga acttgaacga cgcctccacc
      241 gcttcggtgc ctgagttgca gaagaagact cgatccatgc ctggaatcag ccccgtgatc
      301 ttttccatca acatggcgcg ctggtcgttt ggaaaggtct caaagagagt tatcagcgtg
      361 ttcgcctgtt tatagagcgc ctcagcaatt ttcgggtggg catgaccgag gttggcaacg
      421 ccatgtccgg aggagcagtc caaatattcc acaccattct catcgaacaa tgaggcgccc
      481 tgtccacgca cgatggtcag cgtttgtttg gcgtacacgc cagatgtata tttattttcg
      541 atttcggcaa tatgttccat gtttacctca aaacaagatt ttactgatgg ccaataacac
      601 gatgcggcat gtacggctct tccagatata aaatttcttc ctgtgtcaat cggacggaca
      661 atgctccagc cgcctcttcc agatgcgcta tttttgtggc gccgataatg ggcgctgcca
      721 ccggctgttt ttgcaacaac cacgcaagcg cgatatgggt ccgtgagact gcatgctttt
      781 ctgcaagttc agcgacccgt tctataatgg gtcgatctgt ttcggcggcc gaatcatatt
      841 tgcttttttg gatctggtcg gtttcagatc gaagcgtgct ttccgccgac caactccgtg
      901 tcaatctccc ggacgcgagc gggctgtatg ggatcaaccc gatctgctcc tcgcggcata
      961 gtgggatcat ctcccgttcc tcctcacggt aaatgaggtt caaatgattc tgcattgaaa
     1021 tgaagcgcgt ccaaccatgc atttcggcga catgcagcgc cttttgaaac tgccaggccc
     1081 acatggcaga tgcgccaatg taacgcgctt tgcccgcccg aaccacgtca ttcaatgcct
     1141 ccatcgtttc ctcaatcggc gtttcatagt cccagcggtg aatctgatag aggtcgacgt
     1201 aatccgtctc caatcttttt aggctgttat caatttcgac cagaattgcc ttgcgcgaca
     1261 agccgccgcc gttgggagta tcacgcattt ttccatggac cttggtggca atgatgactt
     1321 catcccgttt tgcaaagtct ctcaatgccc gcccaagaat ctcctcgctc gccccaagcg
     1381 aatagacatt ggcagtatca aaaaagttga ttcccatttc aagcgccttc ttgatgatcg
     1441 gacggctgct ctcttcattg agcacccatt tgtgaagcca acgttccgca tcgccaaagc
     1501 ccatacagcc aaggcaaata cgcgagacct tcattccagt agaacctaat cgaacatatt
     1561 ccatgttgtt ctcctaatca gacgcttcca gccttcaatt tgattcaaca aaatgacttt
     1621 gcagactgcg atacttcaat tcctttttat tcaatgcctg aatggcagcc actgctgcgg
     1681 tcgccgcaga aagcgtggtg agcagaggca cgttcattgc aattgccgca gcacggatgc
     1741 gcgctccatc agaatgagca tgcgtaccca gtggcgtatt cacgatcaac tgcaccttac
     1801 cgccgcggat caggtcttca atggcggtgg aaccttcatc aattttatga accacttcaa
     1861 ccaacagacc aaccttgctg aaaaaatccg cagtaccggg cgtggcatac aaggcaaaac
     1921 ccatgcgaga caggtctcgt gccactttca gcgctgcgct cttatcataa tcattaacac
     1981 tgatcagtac cgccccactt tgtggaagtc cctgcccagc tgcgatctgc gatttcgcga
     2041 acgcatgtcc gaagcgcgag gcatgtccca tcacttcgcc agtcgagcgc atctcaggtc
     2101 caagcaatga actggaaccg ggcaatttct tgaacggcag cacggcttct ttaatgaaga
     2161 atccatccac ttgcggttct tcggtcacgc ccaattcttc caatgttttg cccgccatca
     2221 cttgcgcagc tttataagca agattcaaat tcgtggcctt gctggcataa ggcacagttc
     2281 gtgaagcacg cgggttcact tccagcacac acaccacttc atctttcaac gcgaactgca
     2341 cattcataag tccacgaaca ccaagagcaa gcccaagttg ctcggtatat tcgcgcataa
     2401 ttcccaggtg aaattcgctg accttatacg gcggcagcac gcatgccgca tcccctgaat
     2461 gcacacctgc ctcttcgatg tgctgcatga tcgcacccac cacaacactt ccgccatcgg
     2521 acagcgcatc cacgtctatt tcgaaagcat cttccatgaa cttatcgatc aggattggtt
     2581 gccccggtgc cgcctcaatc gccttttgga tgaagcctgc gagatgttct tccgattcga
     2641 ctagcgccat cgcgcgtcca cccaagacga aagagggtct gaccaaaacg ggatatccga
     2701 ttcgttgagc gacttcacgc gcctcttcca acgaccgggc aattccattt tccggctggg
     2761 gaatatccaa ctctttgagc agtttcgcaa actcctcgcg gtcttcggag agttgaatag
     2821 catgaggcga tgttcctaaa atcttcacgc ccgccgcttc gagtccagcc gccaggttga
     2881 gcggagtctg cccaccgaat tggacaatga cgccgatggg attctcttta tcaatcacgt
     2941 tgagaacgtg ctccagagtc aacg
//
LOCUS       DAOQLC010000284         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_18949_length_2961_cov_17.383001, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000284 DAOQLC010000000
VERSION     DAOQLC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18949_length_2961_cov_17.383001"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PL000_21755"
     CDS             <1..1334
                     /locus_tag="PL000_21755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC91565.1"
                     /translation="EPIVKALVERLQNLVDIGLEYLSLSRETDTLSGGESQRVKVVKH
                     LGSSLSDVIYIFDEPSVGLHPRDVHRMNELLQKLRDKGNTVIVVEHDPDVIKVADHIV
                     DVGPHAGPQGGEIVYEGSYINLLKADTITGRHMQQSIPIKDSFRAPMGKLPIKNAKVN
                     NLQNVSVNIPTGVLTVVTGVAGSGKSSLINEVFQHQHPDAIVIDQSAVGVNSRSNPAT
                     YTGILDDVRKAFASANKVQPALFSFNSKGACENCQGLGVIYTELSFFDSVKSPCEVCE
                     GRRFKDEVLAYKLNGKNISEVLSMNVQQALEYFDLPEVKKKLQAMSDVGLDYLGLGQP
                     LSTLSGGECQRIKLASELHKQGSIYIMDEPTTGLHMSDIGHLMQVINRLVDTGNTVVV
                     IEHNLHVIKNADWIIDMGPEGGHKGGQVMFEGTPKELLKAKQSLTSAYLKN"
     gene            1457..2833
                     /locus_tag="PL000_21760"
     CDS             1457..2833
                     /locus_tag="PL000_21760"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC91566.1"
                     /translation="MTTQKILPPSVSELRALFNGKVIAPDDPRYEEARTPFYGGMDKH
                     PAAIVRAADAGDVSRLVSLAREHGFELAVRSGGHSNAGYCTSDGGIVLDLSAMKKLEI
                     DHENQTAWVETGTTAGEYTAAVGTHGFVTGFGDTGSVGLGGITLGGGVGYLVRKYGLT
                     IDHLLAAEVVTADGQLLHVDENSHADLFWAIRGGGGNFGVATRFKFKLHKLDQGYGGM
                     LVLPATADAIVSFLAEADSAPDELSTILNIMPAPPMPFLPEEVHGKLILMAMMFYAGD
                     AAMGEQVISKFRAIATPYADMLRPMAYTEMFPSEEGGYHPVAAGRTMFLDHVDSSVAQ
                     FILDTLAKSTAMMAVTQLRVLGGAYARVPADATAYAHRGSKIMANLAALYGNPEEKEH
                     HEAWVAKYEKKLRQSDKGAYVNFLGDVDQKQVRAGYPGDAWKRLGKIKKKYDPTNLFR
                     LNQNISPK"
BASE COUNT          760 a          727 c          798 g          676 t
ORIGIN      
        1 ccgagccgat cgtcaaggca ctcgtggagc gtttgcagaa ccttgtggat attggtttgg
       61 agtatctcag cctcagccgc gagacggaca cgctttcggg gggcgagtca caacgcgtga
      121 aggtcgtcaa gcacttgggc agcagcctga gcgatgtgat ctatatcttt gatgaaccaa
      181 gtgtaggctt gcatccgcgt gatgtgcatc gcatgaacga gctgttgcaa aaactgcgtg
      241 acaaaggcaa cacggtcatc gtcgtggagc acgatccaga cgtcatcaaa gtggcagacc
      301 atatcgtgga cgtgggtccg catgcgggtc cgcagggagg cgagatcgtc tacgaaggca
      361 gttacataaa cctgcttaaa gcggatacga tcacgggcag gcatatgcag cagtccatcc
      421 cgatcaaaga ttcgttccgc gcgccgatgg gtaagttgcc aatcaagaat gcaaaagtta
      481 ataacctgca aaacgttagt gtgaatatcc caacaggtgt gttgaccgta gtcacaggtg
      541 tggcgggctc tggcaagagt tcacttatca atgaagtgtt ccaacatcaa caccccgatg
      601 cgatcgtgat cgatcaatcc gcagtagggg tgaacagccg atccaaccct gccacgtata
      661 cgggcatcct ggatgatgtc cgcaaggcgt ttgcttcagc aaacaaggtg cagcctgcat
      721 tgttcagttt taactcgaag ggcgcatgcg aaaattgcca ggggctgggc gtgatctata
      781 ccgagttgtc attttttgat agtgtgaaat caccctgcga agtttgcgaa ggcaggcgtt
      841 tcaaagatga agtgttggcg tataagctga atggaaaaaa tatttcagaa gtcttgtcca
      901 tgaatgttca acaagcgttg gagtattttg accttcctga ggtgaagaaa aaactgcaag
      961 ccatgagcga tgtcggtttg gattatctcg gactcggtca gcctttgagc acgctttcgg
     1021 gtggtgaatg tcagcgcatc aaacttgcca gcgagttgca caagcagggc agcatctaca
     1081 tcatggacga accgaccaca ggcttgcaca tgtcggatat tggtcatctg atgcaagtca
     1141 tcaatcgcct tgtcgatacg ggcaataccg tcgtagtcat cgaacacaat ttgcatgtca
     1201 tcaaaaacgc cgattggatc atcgacatgg gacccgaagg tggtcacaaa ggcgggcagg
     1261 tcatgttcga aggcacaccc aaagagttat tgaaagcgaa gcaatcgctt accagtgcat
     1321 acctaaagaa ttaataaaga gtaggggcga cccgtcatgg tttagcctgg acctcgaatg
     1381 agaagaaagg gtcgcccctg ctacgcaaca ccaatttaag aatatttcga cctgtagaca
     1441 atttggagac tttaacatga cgacacaaaa aatacttccc ccatctgttt cagaattgag
     1501 agcactcttc aacggcaagg tcatcgcgcc cgatgacccg cgttatgaag aagcacgcac
     1561 tcccttttat ggcggcatgg ataaacatcc cgcagcgatt gtccgcgccg cagatgcggg
     1621 tgatgtatcg cgtctcgttt cgttggcgcg tgaacatggc tttgaactcg ccgttcgcag
     1681 cggtggtcac agcaatgcgg gttactgtac tagcgatggc ggtattgtgc tcgacctctc
     1741 tgcaatgaaa aaattggaga tcgatcacga gaatcaaacc gcctgggttg aaactggcac
     1801 gactgctggc gaatacacag ccgctgttgg cacacatgga tttgtcaccg gctttggcga
     1861 tacgggttcg gtcggtctcg gcggcattac gcttggcggc ggcgttggct atctcgtccg
     1921 caaatatggg ttgaccatcg atcatctgct cgccgcggaa gtggtcacag ccgatggaca
     1981 actcttgcac gtggatgaaa attctcatgc cgatctcttc tgggcgatcc gcggcggagg
     2041 cggcaacttc ggcgtggcga cgcgcttcaa gttcaagctt cataagttgg atcaaggcta
     2101 cggcgggatg ttagtcctgc cagccacagc ggatgccatc gtttctttct tagccgaagc
     2161 tgattctgct cccgatgaat tgtccaccat cctgaacatt atgcctgcgc cccctatgcc
     2221 tttccttcca gaagaggtgc atggcaaatt gatcttgatg gcgatgatgt tctatgccgg
     2281 tgatgcagca atgggggagc aggtcatctc caagttccga gccattgcca ctccttacgc
     2341 ggacatgctg cgtccgatgg cgtatacaga aatgttccca tccgaagaag gcggctatca
     2401 ccccgttgct gcggggcgca ctatgttcct tgaccatgtg gatagttctg ttgctcagtt
     2461 cattcttgat accttagcca agtctacggc gatgatggca gtgactcaac tgcgcgtgtt
     2521 gggcggagcc tatgcacgcg tccctgccga tgcgaccgca tatgcccatc gcgggtcaaa
     2581 gatcatggca aaccttgccg ccttgtatgg caacccggaa gaaaaagaac atcacgaagc
     2641 ctgggtcgct aaatacgaaa agaaactgcg tcaaagcgac aaaggcgcgt acgtcaactt
     2701 ccttggtgat gtggaccaaa aacaagtccg cgcggggtac ccgggtgacg catggaagcg
     2761 cctcggtaag atcaagaaaa aatatgaccc gaccaatctt tttcgtctca atcaaaatat
     2821 ttcgccaaag taataaaaaa taaatgaaac catctcaata aggagcatgt accatgaatg
     2881 tggaaccaaa gttagaacat cgtaaggaac aaccatatgt agcagtccgc gtacaagtag
     2941 ggataccctt tggtgaggtg c
//
LOCUS       DAOQLC010000285         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_19745_length_2893_cov_15.390063, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000285 DAOQLC010000000
VERSION     DAOQLC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19745_length_2893_cov_15.390063"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1277
                     /locus_tag="PL000_21765"
     CDS             201..1277
                     /locus_tag="PL000_21765"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC91567.1"
                     /translation="MARAGIYKSEVVRARNNLLAMGRYPSIDAIRGELGNTGSKGTIH
                     RYLKEIEEEEGGGTGPQGAVSEAIQDLAARLAERLHEEADQRVAAMTDKHKAEIAALN
                     DTTTALRHEVESFRSQSERQALELAAEKTAHAETLATLQEERVVRAQMAQRIQDMEGQ
                     QAKDEAHRVSLEEKHQHAREALEHFRTAAKEQREQDQRQFEQQIQFLQGELRAAKETV
                     NAKQQELIRSHQDNARLSSEQGHLRSELHRLETEVRLLRTALEKLAVSELKNQQMEER
                     LAQSATRIDELVVENQSSTEKLLDANQAGLRLATELTAAKAVLASQEQILQKLTVNQA
                     PATKSSGPRKESAENQESLFDPEK"
     gene            1378..2181
                     /locus_tag="PL000_21770"
     CDS             1378..2181
                     /locus_tag="PL000_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91568.1"
                     /translation="MLISEQNTEGYMESNEYIRPPGTYYDIVAHPIDRRRAIEVAIVQ
                     DHRFAFFYWLKWRNKQAEQPNPPTLISLDWHQDLGQPCDLECEWLSTLDTTDYKAVGY
                     FCWDKLHVLNDGHILAAAYLNLIGDIHVVQKQRDECPEEFVDIEGRKHLVRCYDSVEQ
                     LTEAISGQEHDSVILDIDLDYFTESPDPCGGGDKLQLVGQEEIQACLDPASPFMSWIF
                     PRMTGMTIATEPEFCGGLMNSNHLFNVVCGTLFDPPLLSHKAGWRHLSK"
     gene            complement(2577..>2893)
                     /locus_tag="PL000_21775"
     CDS             complement(2577..>2893)
                     /locus_tag="PL000_21775"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91569.1"
                     /translation="IADVTQGAALTVGIPTTAVTADKGYDSDELVETIEATGAMANIP
                     PRSNRKNKRPVDWHRYKARHLVECFFNRLKQFRRIATRYDKLANRFNAFLHLACAYVW
                     LL"
BASE COUNT          759 a          749 c          772 g          613 t
ORIGIN      
        1 caccattttc tcaagtcgcc gctcagtttg cttcggaatt attcagcgtt tcctttacat
       61 aatttttctg ctccatggcc aatcacaaca aataatcaac cattatcatg tcttatgatt
      121 tcgactttgc atgtattgaa ttctgtagat ttggtatgta atatcatatt acgtaataca
      181 gtacgaaatt cgaggatgcc atggccagag ctggaatcta caaatcagaa gtcgtcagag
      241 cacgtaacaa cctattggcg atgggacgtt atccatcgat cgatgccatc cgtggcgaat
      301 tggggaatac cggctcgaaa ggcacgatcc accgctatct gaaggaaatc gaggaggagg
      361 aaggtggggg caccggtcct cagggcgcgg tcagtgaggc cattcaggac ttggccgcca
      421 ggctcgccga acgcctgcat gaggaagccg atcaacgcgt ggcggcaatg accgacaagc
      481 acaaagcgga aatcgctgcg ctgaacgaca ctaccaccgc gctacgtcat gaagtcgaat
      541 ccttccgcag ccagtctgag cgccaagccc tagagttggc ggcagaaaaa acggcccacg
      601 ccgaaactct ggccacgcta caggaagaga gagttgttcg ggcgcaaatg gctcagcgca
      661 tccaggacat ggaggggcaa caggcaaagg atgaagcgca ccgtgtatca ctggaagaaa
      721 agcaccagca tgccagagag gcgctggagc acttccgtac ggcggcgaag gaacagcggg
      781 agcaggatca gcgacagttc gagcagcaga tccagttctt gcagggcgaa ctgcgtgcag
      841 ccaaggagac ggtgaacgca aaacagcagg aactcatccg cagccaccag gacaatgccc
      901 gcctttccag cgagcagggt catcttcgca gcgagttgca tcgcctggaa accgaggttc
      961 gactactacg tactgccctg gaaaaattag ctgtttccga actcaaaaat caacagatgg
     1021 aagaacgcct ggcccagagt gccacacgca ttgatgaact ggtcgtggaa aaccaaagca
     1081 gcacagaaaa attactggat gcgaatcaag ctgggctccg cctagcgaca gaattgaccg
     1141 cagcgaaggc tgtgttggcc agccaggaac aaattctgca aaagctcacc gtcaatcagg
     1201 caccggcaac gaaaagcagc ggaccgcgca aagaaagtgc cgagaaccaa gaatcgctct
     1261 ttgacccgga aaaataatga ggaaggtcag catcgtgcgg caatattttg accagcccgc
     1321 acgctgtttg ccagctaccc aagcgtgagt atctggttgc atggtttcaa cgctaccatg
     1381 ctcatcagcg aacagaacac agagggatac atggagtcca acgagtacat tcgaccacct
     1441 ggaacctatt acgacatcgt tgcacatccg atcgatagga ggcgtgcaat cgaagtggcg
     1501 atcgttcagg atcatcgttt cgctttcttc tattggctca agtggcgcaa caagcaggct
     1561 gagcagccca atccgccaac actaatttct ctggactggc atcaagatct tggtcaaccc
     1621 tgcgacctcg aatgcgaatg gctcagcacg cttgatacga ccgactacaa ggctgtcggt
     1681 tacttttgct gggataagtt acatgtcctt aacgatggcc atatcctggc tgctgcttat
     1741 ctcaatttga ttggcgacat ccacgttgtg caaaagcaac gtgatgaatg cccggaggaa
     1801 ttcgtggata tcgaagggcg gaagcatctg gtccgctgtt atgactccgt tgagcaacta
     1861 accgaggcga tttcagggca agagcacgat tcggttatcc tcgatatcga tctggattac
     1921 ttcaccgaaa gtccggaccc atgtggagga ggcgacaagc ttcaactggt cggccaagaa
     1981 gaaatccagg catgccttga tccagcaagt ccgttcatgt cgtggatttt cccgcgaatg
     2041 acgggaatga ccatcgcgac tgagccagaa ttctgtggcg ggttaatgaa ctcgaatcac
     2101 ttgttcaacg tggtgtgcgg cacgttattt gatccgccac tgttgagcca caaggccggc
     2161 tggcgccatc tgtctaaata gtcgaagcca gttttttctc taaatcagat ggttagcaga
     2221 gtttttcgag gatttcacat gaatcccggc cgaccctcga taccccggct caagcctgct
     2281 tggctgacaa gcctacaatt cagacgaatt cctggcgtgg ctcaagatgg gcgacgccgt
     2341 cgcggtcatt ccgtccaagg cccacgaaac ggtacaaaga gcgtacgatc ggtggcttta
     2401 cgagggacgc tacgtcatcg aatgcctgtt tggaccgctc aaatatttcc gccaaatcgc
     2461 cactcgatgc gaaaacccat gaatttcaat ggttttctca cattttcagc ggctttgctg
     2521 tggctgcgat gaactgcggc aaaagatcaa caaagcctgc aaagcctagg gcgtgttcac
     2581 agtagccaga cataagcaca agcgagatga agaaaggcat tgaatcgatt ggcaagtttg
     2641 tcatatcggg tcgcgatacg acgaaactgt ttgagtcgat tgaagaaaca ctcgaccaga
     2701 tgacgagcct tgtagcgatg ccaatcgaca ggacgtttgt tcttccgatt gctccgggga
     2761 gggatgttgg ccatcgcccc ggttgcctcg atggtttcga ccaactcatc gctgtcgtag
     2821 cctttatcgg ccgtgacagc ggtggttggg ataccgacag tcaaggcggc gccctgcgtc
     2881 acatcggcga ttt
//
LOCUS       DAOQLC010000286         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_19902_length_2880_cov_22.550442, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000286 DAOQLC010000000
VERSION     DAOQLC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19902_length_2880_cov_22.550442"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2880)
                     /locus_tag="PL000_21780"
     CDS             complement(<1..>2880)
                     /locus_tag="PL000_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91570.1"
                     /translation="SSYTVKVNTGSGNGTIRLDVKAGGIFDSALNPLDPPFITGHVYT
                     VAKTSSLLVSPVNDTYLTLTKPVFTWKTITGALEYQLQVDETEWFTTPEVNQSQPSGT
                     TYTSPELDYGTHYWRMRVRTSGGWGVWTAGWKFTLTPALPAAPTLASPASAAVLADNT
                     PLLAWNMVLADDRYEVQVDNLSTFASPEQTFTTALDALSYTATALPDGVWYWRVRAIN
                     YLNLPGAWSTSRSFTVDTVAPPAPALSAPADDATIIGTPAFTWAAAATATKYQFEYDS
                     DQDFSSPDYTSAELTTTSHIPPAIPLGSYSWHVRGKDAAGNWSAWSAPRVVTILSQIP
                     VAPTLGAPVNAFATNDATPEFSWLAVASGNTYQLEISNATTFTTKQQTFTGGVGVLNY
                     TATTIPDGVWYWHVRAVNVDGAAGAWSAYRSFTIDTTAPVTAPALSAPANAASVIGTP
                     AFSWSSVATAAKYEFQYDDDADFSSPVYTSAELTTLNHTPPAMVLDSYSWRVRARDAV
                     GNWSAWSTARTITILPPVPVAPALTSPAASVVTNDSTPDFVWASVVSGDTYQLEISNA
                     STFATKQQTFTGGVGVLNYTATTIPDGVWYWHVRALNVNGVAGAWSAYRSFTVDTTGP
                     VAPVLSAPANAAAVIGTPAFSWVAAATASKYQFEYDNDADFSSPIYNSGDLTTTSHTP
                     PAMALGLYSWHVRAKDAAGNWGVWSAARTVTILPLVPVAPTLTSPAASAVTNDSTPDF
                     VWAAVASGETYQLEISNASTFATKQQTFTGGVGVLSYTATPIPDGVWYWHVRAVNVNG
                     VVGAWSAYRSFTVDTNGPSAPTLSAPANAAMVTGTPAFSWAATTTAVKYQFEYDNDAD
                     FSSPVYNSGDLTTTSHTPPAMALGLYSWHVRARDAAGNWGPWSLTRTITIQPLVPVAP
                     TLTSPAASAVTNDSTPDFVWASVASGDTYQLEISNASTFATKQQTF"
BASE COUNT          661 a          755 c          874 g          590 t
ORIGIN      
        1 gaaggtctgt tgtttggtgg caaaggtgct cgcgttcgaa atttcaagct ggtaggtatc
       61 gccagaggcc actgaagccc aaacgaaatc aggagtcgag tcattggtta ccgcagaggc
      121 ggcagggctg gtcagggtcg gggcaacagg aaccaacggc tggatcgtaa tcgtacgtgt
      181 caaactccac ggaccccagt tgccagccgc atccctcgcg cggacatgcc atgagtaaag
      241 gcccaatgcc atagcgggcg gggtgtgact tgtggtggtc agatcgccag agttgtagac
      301 tgggctggag aaatccgcat cgttgtcgta ttcgaactgg tatttcactg cggtcgttgt
      361 ggctgcccag gagaacgccg gggtgcctgt taccatggcc gcattggctg gcgcactcaa
      421 ggttggtgcg gaaggcccgt tggtatccac tgtgaaggaa cgataggcgc tccaggcacc
      481 aaccacgcca ttcacattca cagcacggac atgccaatac cacacgccat cggggatggg
      541 tgtggcggtg tagctcaaga cgcccacacc acctgtgaag gtctgctgct tcgtggcaaa
      601 ggtactcgcg ttcgaaattt caagctggta ggtttcacca gatgccactg ccgcccaaac
      661 gaaatcggga gtcgagtcat tggttactgc agaagccgca gggctggtca aagtcggcgc
      721 aacaggaacc aacggcagga tcgtcacggt gcgggcagcg ctccatacac cccaattgcc
      781 agctgcatcc ttcgcgcgga catgccatga gtaaaggccc aatgccatgg cgggcggggt
      841 gtggctggtg gtggttagat cgccagagtt gtagatcggg ctggagaagt ccgcatcgtt
      901 gtcatattcg aactggtatt tcgaagcggt ggcagcagca acccaggaga aggcgggtgt
      961 accgatcact gcggcggcat tggctggtgc gctcaacaca ggggcaacgg gacccgttgt
     1021 atccaccgtg aaggagcggt atgcactcca ggcacccgcc acaccattca cattcaacgc
     1081 acgaacatgc caataccaca cgccatctgg gatggtcgtg gcagtgtagt tcaagacacc
     1141 cacaccacct gtgaaggtct gctgtttcgt ggcgaaggtg ctcgcattcg aaatttcaag
     1201 ttggtaagta tcgccagaga ccaccgaagc ccaaacaaaa tcaggagtcg agtcattggt
     1261 taccactgag gcggcggggc tggtcaaggc cggggcaacg ggaacgggcg gcaggatcgt
     1321 aattgtccgc gctgtgctcc aggcgctcca atttccaacc gcatccctgg cgcgtacacg
     1381 ccatgagtag ctgtccaata ccattgcggg cggtgtgtga ttcaacgtag tcaattcagc
     1441 cgaggtgtaa accgggctgg agaaatccgc atcatcatcg tactggaatt cgtatttcgc
     1501 ggctgtggca acactgctcc atgagaaggc aggtgtgcca atgaccgatg ccgcattggc
     1561 cggagcactc aaggcagggg ccgtcactgg cgcagtggta tcaatggtaa aggaacggta
     1621 ggcactccac gcacctgctg caccatccac attcacagca cgaacatgcc aataccacac
     1681 accatcgggg atggtcgtgg ctgtgtaatt caagacaccc acaccacctg tgaaggtctg
     1741 ttgtttggtg gtgaaggtag tcgcgttcga aatttcaagt tggtaggtat taccagaagc
     1801 cactgcaagc caactaaatt caggggtggc atcgttggtg gcaaaggcgt tcacaggagc
     1861 accaagagtc ggcgcaacag gaatctgcga gaggatggtc acaacccgag gcgcactcca
     1921 ggcactccaa ttacctgccg catcttttcc acgcacatgc catgaataag accccagcgg
     1981 aatggcaggc gggatatggc tggtggtggt caattcagca gaggtgtaat ccgggcttga
     2041 gaaatcctgg tcactatcat attcaaattg atatttcgtt gcagtggcag ccgctgccca
     2101 agtgaaggcg ggagtaccaa tgatggtggc atcatcggct ggggcactca aggcaggcgc
     2161 aggcggtgca accgtatcca cggtgaagga acgcgaagtg ctccaggcac cggggaggtt
     2221 caagtagttg atcgcacgaa ctcgccaata ccaaacccca tcgggcaggg ctgtggcagt
     2281 gtatgaaagg gcatccaatg ccgtggtgaa ggtttgttcc ggtgaggcaa aagtgctgag
     2341 gttatccacc tgcacttcgt agcggtcatc cgccagaacc atattccagg cgaggagcgg
     2401 agtgttatcg gcgagaaccg ctgcactggc cgggctggca agtgtcggcg cggcaggaag
     2461 cgcaggagtc agggtgaatt tccagcctgc tgtccataca ccccatccac ctgatgtccg
     2521 cacgcgcata cgccagtaat gagtcccata atccaactcg gggctggtgt aggtcgtgcc
     2581 gctcggttga gattgattca cttcaggggt cgtgaaccat tcggtctcat ccacttgtaa
     2641 ctggtactca agcgcgccgg tgatggtctt ccaggtaaag actggcttgg ttaatgtaag
     2701 gtatgtatcg tttacagggc ttacgagcag actgctggtc tttgcaacgg tgtagacgtg
     2761 gcctgttatg aacgggggat caagcgggtt aagggcgcta tcgaaaattc cacccgcttt
     2821 gacatccagg cggatcgttc cattcccgct gcctgtgttg accttaacgg tatagctact
//
LOCUS       DAOQLC010000287         2879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_19920_length_2879_cov_23.118980, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000287 DAOQLC010000000
VERSION     DAOQLC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19920_length_2879_cov_23.118980"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1143)
                     /gene="aroF"
                     /locus_tag="PL000_21785"
     CDS             complement(115..1143)
                     /gene="aroF"
                     /locus_tag="PL000_21785"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC91571.1"
                     /translation="MMIIMRPGAPREQIDAVIAAVESHKLSAHPIFGVEQTIIGAVGD
                     GHYVLKETFEAFPGVLEVQRISKPYKLASRQFHEQDSVFEFNGFTIGGTEVPIIAGPC
                     SVEGRTQILEVAQAVKEAGANALRGGVFKPRTSPYAFQGLGEEGLEYMAEAREKTGLP
                     LVVEVMAVSQIEMMEKYVDIFQLGARNMQNFNLLRALGETRTPVLFKRGMSATIEEML
                     MASEYILNGGNHKVILCERGIRTFETATRNTTDINAVPVLKKLTHLPVVIDPSHSTGD
                     SAFVSSVAKAGVAAGADGLIVEVHQDPPHAISDGKQSLTPEAFAKMVKQVKAVAEAVD
                     RKLVIGNS"
     gene            complement(1248..2306)
                     /gene="aroF"
                     /locus_tag="PL000_21790"
     CDS             complement(1248..2306)
                     /gene="aroF"
                     /locus_tag="PL000_21790"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC91572.1"
                     /translation="MMIIMKANATPQQVEAVIASVKSAGLNVHLSQGVEATIIGAIGE
                     THNIPMERFESLDGVDLVQRITQPYKLASRQFHPEDSVISMNGFSVGGNEVAVIAGPC
                     SVESRSQIIETAIAVKEAGASALRGGVFKPRTSPYSFQGLGEEGLELLAEAREKTGLP
                     IVVEIMSQVQLDLMVKYVDVLQVGARNMQNFNLLRAIGESRTAVLLKRGLSATVEELL
                     MSAEYILAGGNTRVMLCERGIRTFETSTRNTTDINAIPVLKNLTHLPVILDPSHSTGL
                     ANYVAAISRAGIAAGADGLIIEVHPDPAKAVSDGKQSLKPEAFAAMVKQVGQIAQIMG
                     RGLAGAQSNIQPLKQGWA"
     gene            complement(2395..2766)
                     /gene="aroH"
                     /locus_tag="PL000_21795"
     CDS             complement(2395..2766)
                     /gene="aroH"
                     /locus_tag="PL000_21795"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNC91573.1"
                     /translation="MAIRGIRGATTVAEDEPELILQATRELLEAVIKENAGMKPEDVG
                     SAIFTVTDDLASTFPAQAARQMGWSMVPMMCAREIPVPGSLPRAIRVLVHWNTETPQK
                     DIKHVYLRDAVKLRPDLVAAQ"
BASE COUNT          653 a          750 c          759 g          717 t
ORIGIN      
        1 cgcgcagtcc taatgccagc gaaccgccca tcaagcccaa tccgatgatg gcgattttag
       61 attcagagag tgtaaagttt gattcatcca tgattggtaa atagtaattg gtaattacga
      121 attaccaatt accaattttc gatctaccgc ttcagccacc gctttgacct gcttcaccat
      181 tttggcaaag gcttcgggtg tgagcgactg tttgccatca gagatggcgt gtggcggatc
      241 ctgatggact tcgacgatga gaccgtcggc gcctgcggca acacccgctt tggcaactga
      301 agaaacaaaa gcggagtcgc ctgtggagtg actggggtca atcacgacgg ggagatgcgt
      361 gagcttcttg agaacgggca cagcgttgat gtcggtggtg ttgcgcgtgg cggtttcgaa
      421 ggtgcgaatg ccgcgctcgc acaagatgac cttgtgattg ccgccgttga gaatgtactc
      481 gcttgccatg agcatttctt cgatggtggc agacatgccg cgtttgaaaa gcacaggcgt
      541 gcgcgtctca ccgagagcac gcagcaggtt gaagttctgc atgttgcgcg cgccgagttg
      601 gaagatgtcc acatattttt ccatcatctc aatttgagag accgccatta cttcgacaac
      661 taaaggcaag ccagtctttt cgcgggcttc cgccatgtat tcgagacctt cttcgccgag
      721 accttgaaag gcatacggtg aagtgcgcgg tttgaaaaca ccgccgcgta aagcatttgc
      781 acccgcttct ttgacggctt gtgccacttc gagaatctgc gtgcgtcctt ccactgaaca
      841 cggacccgca atgatgggaa cttccgtgcc accgatggtg aagccattga attcgaaaac
      901 ggaatcctgt tcgtggaatt gacgtgatgc caatttatac ggcttggaaa tgcgctgcac
      961 ttcgagcaca ccggggaagg cttcgaaggt ttctttgaga acataatgtc cgtcaccgac
     1021 tgcgccgatg attgtttgct ccacgccgaa gatggggtga gcggataact tatggctttc
     1081 aacggcagca atgactgcgt caatttgttc gcggggtgcg ccggggcgca tgatgatcat
     1141 catggtggta tggtttcctt tttagtaatt ggtgattaag attagtaaat agtaattggt
     1201 aattagagat aggtaattac tatttaccaa ttatctatct ctgctcttta cgcccaacct
     1261 tgctttaacg gttggatgtt gctttgggcg ccagccagac cgcgccccat aatttgcgcg
     1321 atctgcccga cctgcttgac catcgcagcg aaggcttcag gcttgagcga ttgcttgcca
     1381 tcagaaactg ctttggcagg gtcggggtgg acttcaatga tgagtccatc tgcgcctgct
     1441 gcaatgcctg cacgagaaat cgcagcgacg taatttgcaa gacccgtgga gtgactgggg
     1501 tcaagaatga caggcaggtg agttaagttt ttcaaaacag ggatggcatt gatatcggtt
     1561 gtattgcgtg tggacgtttc aaacgtgcgg atgccgcgct cgcacaacat cacacgggtg
     1621 tttcccccag ccagaatata ttcagcagac atcagcagtt cttcgaccgt ggcagagagt
     1681 ccgcgtttga gcagaacggc agtccgcgat tcaccgatgg cgcgcagcag gttgaagttc
     1741 tgcatgttgc gcgcgcccac ctgcagcaca tctacatatt tcaccatcag gtccaactgc
     1801 acctgcgaca tgatctccac cacaatgggc aagcctgttt tctcacgtgc ttcggcaagc
     1861 agctcaagac cctcttcgcc cagtccctgg aaggaatacg gtgatgtgcg cggcttgaac
     1921 actcccccac gcaaagcgga cgcgcccgcc tctttcacag cgatggctgt ttcaatgatc
     1981 tgagagcgac tctccactga acatggtcct gcaatcacag caacctcatt gccgcccacg
     2041 gaaaatccgt tcatcgaaat gaccgaatcc tcggggtgga attggcgcga cgccagcttg
     2101 taaggctggg taatgcgttg gacaaggtcc acaccatcga ggctttcgaa gcgttccatc
     2161 ggaatgttat gcgtttcacc gatggcaccg ataatggtag cttcgacacc ttgcgaaagg
     2221 tggacattca atcccgccga tttgacggat gcgatcaccg cttccacttg ctgcggggtt
     2281 gcgttggctt tcataatgat catcataggt tcttctccta aaaagtttca ggttgaaagt
     2341 tgaaggttac aggttgataa actttcaaca ttcaaccttg aaccttcaac cgacttactg
     2401 cgcggcaacc aagtcgggtc ttagcttcac cgcgtctcgc aaatacacat gcttgatatc
     2461 tttttgcggc gtttccgtat tccaatgcac cagcacacgg atcgcccttg gcaaactacc
     2521 agggacggga atctcgcggg cacacatcat cggcaccatg ctccagccca tttgacgcgc
     2581 cgcctgcgcg gggaaggtgg aagcgaggtc atcggtcaca gtaaagatgg cactgccaac
     2641 atcctcaggc ttcatacctg cattctcctt aatgaccgct tcaagcaact ccctcgttgc
     2701 ctgaaggatc aattctggtt cgtcctctgc gacggtggtt gcgccgcgaa ttccacgaat
     2761 tgccatcttt gcgctccttt tattcccctc tccctctgga aaagagggtt tggggtgagg
     2821 tgagaataaa aaagagcgag accgtgtggt ctcgctcttt tgcatacagt gtttgctgt
//
LOCUS       DAOQLC010000288         2858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_20162_length_2858_cov_24.918302, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000288 DAOQLC010000000
VERSION     DAOQLC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20162_length_2858_cov_24.918302"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..2774)
                     /locus_tag="PL000_21800"
     CDS             complement(387..2774)
                     /locus_tag="PL000_21800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNC91574.1"
                     /translation="MQMETDVTSTIDVNLPRSISVRSLVEFVMQSGDLSTGGFQRRDR
                     AQLGTQGHKRVQRSRPEGYETEVEVRCQINSGDMPLEVRGRIDGLYASHEPVIIEEIK
                     TTSLSLDLVSEDHNPLHWGQAQCYAYMYARQHRLDEVSIHLTYYHLDSHEEKTFVRHF
                     TWTGLEDFFSGLITSYLDWFRKVQAWQLQRDRSIEKLEFPYEEYRPGQRDMAVAVYKA
                     IRAKDRAYIQSPTGVGKTIATLFPSVKALGQGLATKIFYLTAKTSGRLVAEKALEDMR
                     QSGLYLRSVTLTAREKICFCPPVNCDPQVCIFARGYFDKVKSALAELAQHQTFTRPLI
                     EEIARKYEICPFEFSLDLALWVDCIICDYNYVFDPRVYLHRFFDFTTEPYIFLVDEAH
                     NLPDRARAMYSAELEKEKVLQLQRALKPSAPGLAKKLGEVNKLLLEKRKACEGEGRDV
                     LIEHELPEALLKAIREFVNKAEDWLVQNQVAEFRQELLEFYFLCSNYLRTADYFDTFY
                     VSYFERVGQSNLRAKLFCLDPAPMLTVPLERSRSTIFFSATLLPMDYFMKLLTGAAEH
                     PRRIFHSPFPQENVCLLVHNKISTKFVHRADSYDAIAEAIETVLNTHVGNYLVYFPSY
                     VYLNEVVERLKGRLPAEQLLVQERGMTEEARDAFLAQFSSANEETLVGFAVMGGVFGE
                     GIDLVGERLIGVVVVGVGLPQLGLERDLIKGYFDQHSASGFAYAYQYPGFNRVLQATG
                     RVIRTDTDQGIIVLIDERFAQAHYRQLFPSHWRDYKIVKNTQEMKDHLTRFWSQK"
BASE COUNT          736 a          758 c          668 g          696 t
ORIGIN      
        1 aagaagccga atccgcagct gacgccatgc gccctggctt ccagcaggcg ttggagggga
       61 aggtacaagg tccgcccagt gttcgtatgt ttgaggtctt tgaaccttag gggttccgca
      121 agatatatca tgaatacggg tataaacttt gacctgctcg gtttacgaga ataacaaagc
      181 caatttaatg aaagaacacc cctatgcata ggggtgttct cgttttagaa cattattgta
      241 aataatcaat ccacttctcg tccgtcgcag gctgacacct gagttgctga gtgttgtgga
      301 gttgtttgca aagcggacaa aaaaccagac ataaaagttg ggggcattgc aggcttttag
      361 ggctgaacta ctcggaggtg tcaagctcac ttctgtgacc aaaatcttgt caaatgatct
      421 ttcatctcct gggtgttttt tacaatttta tagtcgcgcc aatgagaagg aaacaattgg
      481 cggtagtgag cctgggcaaa acgttcatcg atcaacacaa tgatgccctg atctgtatcc
      541 gtacgaatga cccgacccgt tgcctgcaat acccgattga accccgggta ttgataggca
      601 taggcaaatc cgcttgcaga gtgttgatca aaataccctt tgatcaagtc ccgttccaat
      661 cccaactggg gcaatcccac accaacgacc acaaccccga tcaatcgttc tccaaccaga
      721 tcgatccctt cgccaaaaac tccacccatc acagcgaagc cgaccagtgt ttcttcattg
      781 gctgaagaaa attgagccag aaatgcatca cgagcctctt ccgtcatccc gcgttcctga
      841 acgagaagct gctccgcggg caatctccct ttgagccgtt ccaccacctc attcaaatag
      901 acataagacg gaaagtacac cagataattc cccacatgag tattgaggac ggtttcaatg
      961 gcttcggcaa tggcatcgta agaatcagcc cgatgcacaa atttggtgga gatcttgtta
     1021 tggaccagca aacagacatt ctcctgcggg aagggggaat gaaaaatgcg cctgggatgt
     1081 tctgcagcgc ctgtcaggag tttcataaaa taatccatcg gcagcaatgt ggctgagaaa
     1141 aagatggtgg agcggcttcg ttccaacggg accgtcaaca tgggtgctgg atcgaggcag
     1201 aataactttg cccgaaggtt tgattgcccc acccgctcaa aataagaaac atagaaagtg
     1261 tcaaaataat cagcagtacg caggtaatta ctgcacaaga aataaaattc cagcagctcc
     1321 tgcctaaact ctgccacctg attctgtacc agccagtctt ccgccttatt aacgaactct
     1381 cgaatcgcct tgagcagcgc ttcagggagt tcatgctcga tcaacacatc cctgccctcc
     1441 ccttcacagg ctttgcgctt ttccagcaat agcttgttga cctctcccag cttttttgcc
     1501 agccccggtg cagatggttt caaggcgcgc tgaagttgca gcactttttc cttttccagt
     1561 tcagccgagt acatggccct ggcacggtct ggaagattat gcgcttcatc aacaagaaag
     1621 atatatggct ctgtggtgaa atcgaaaaaa cggtgaagat atacccgcgg atcaaaaacg
     1681 tagttgtaat cacagatgat gcaatccacc cacaaggcca gatccagcga aaactcaaat
     1741 ggacagatct catactttcg ggcgatctct tcaatgagcg ggcgggtaaa tgtttgatgc
     1801 tgggcaagtt ctgccagcgc gcttttcact ttgtcaaaat atccacgggc gaagatgcac
     1861 acttgcgggt cgcaattgac cgggggacaa aagcagatct tttccctggc ggtcagtgtc
     1921 acactgcgaa gatacaaccc cgactgacgc atatcctcca gcgctttttc cgccaccagc
     1981 cgccccgatg tctttgccgt caggtaaaaa attttggttg ccaggccctg ccccagcgct
     2041 ttgaccgatg ggaagagggt cgcaatcgtt ttccccaccc ccgtcggcga ctggatataa
     2101 gcacgatctt tcgcccgaat ggccttgtac acggcaaccg ccatatcccg ctgaccgggg
     2161 cggtactctt catagggaaa ttcaagtttc tcaatggaac gatcccgctg gagttgccat
     2221 gcttgaactt tgcgaaacca atccagatac gaggtgatca aacccgagaa aaaatcctcc
     2281 aaacctgtcc atgtaaaatg gcgtacaaag gttttttctt catgactatc caaatgataa
     2341 taggtcaggt gaatactgac ttcatccagt cgatgctgcc gggcatacat gtaggcatag
     2401 cattgcgcct gtccccaatg cagcggatta tggtcttcac tcaccagatc caggcttagt
     2461 gaagttgttt tgatctcctc gatgatgacg ggttcatggc tggcatacag gccatcgatc
     2521 cgcccccgta cttccaatgg catgtcaccg ctgttgatct ggcagcgaac ctccacttca
     2581 gtttcataac cttcggggcg ggaacgctgg actcgcttat gtccctgcgt tccaagttgg
     2641 gcacggtccc gcctttgaaa tccgcccgta gacaggtccc cggattgcat cacaaattcc
     2701 accaggcttc taaccgagat gcttcgcggc aggtttacat cgatcgtaga ggtgacatca
     2761 gtttccattt gcatctggaa ttctagcatg agttgaactg ggtcaaccca tgttggaagg
     2821 cttttttaca accttcccaa gtcacgttgg tccgcacc
//
LOCUS       DAOQLC010000289         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_20579_length_2828_cov_24.666787, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000289 DAOQLC010000000
VERSION     DAOQLC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20579_length_2828_cov_24.666787"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PL000_21805"
     CDS             complement(<1..984)
                     /locus_tag="PL000_21805"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC91575.1"
                     /translation="MGQEHIFISHSSKNDDVVKRLREILELEKFELWVDSREFTGGDI
                     LEDTLQEQIKTAKSFIILLSIEALSSEWVQKELKLAHKVAKERKDEGYKVISIIMPGV
                     PAGLLKPFFPKEPIHIFLEKGATGPDLDSKMPHILAALGKELPNDWEPPTLIKAAPVE
                     ELILELTDPIIEEKEGIRRATATAVLTYNPADNSRAIESKRYRFTAPLGAVELGEIRW
                     YIEKYYQWPTGVFKTRAEKTEKALPEWGNALYKAALGGESAREPFEAWKRTSGSRRLS
                     VQVDGEPLEGTEEAKAALIREAASDLLSLPWEIMHDGVGYLSQGANGVRVRR"
     gene            1238..1573
                     /locus_tag="PL000_21810"
     CDS             1238..1573
                     /locus_tag="PL000_21810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC91576.1"
                     /translation="MRRDVFQAIADPNRRAILSLLARQRMTLNGVADNFRISRPAVSK
                     HIRILKECGLVVVIPQGRERFVEARFDKLNEVTDWVEQYRQLWETRFNRLDDLLEMMK
                     QEKKNALEK"
     gene            1557..2039
                     /locus_tag="PL000_21815"
     CDS             1557..2039
                     /locus_tag="PL000_21815"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC91577.1"
                     /translation="MPSKNKVTVTAEPGTQELFIVREFDAPRELVYKAHIDPKLYVQW
                     LGPHGYEMVLETFEPTNGGKYRYVHKDKDGNEYGFHGSFHAMSIENMIQTFEFEGYPG
                     HVSLDCMTLEELPGNRTKATIHSIFQSVSDRDGMIQNGMEKGVSEGYERLDDILKEMK
                     "
     gene            2040..2591
                     /locus_tag="PL000_21820"
     CDS             2040..2591
                     /locus_tag="PL000_21820"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HNC91578.1"
                     /translation="MNGYLVLLRGINVGGKNIIKMADLKASFEEMGFSKVVTYIQSGN
                     VVLQSEDKDKAVLTTRIEKGLSKRFNFEAKVVVISQKELAAVVKSAPKGFGEDDAKFR
                     YDVIFLKEPLTPKEAMESVKVREGVDTAHAGKQALYFSRLISRASQSYLTKIIGTPVY
                     QNMTIRNWNTTTKLLALMEGQDG"
     gene            2584..>2828
                     /locus_tag="PL000_21825"
     CDS             2584..>2828
                     /locus_tag="PL000_21825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91579.1"
                     /translation="MAKTNFQSIDEYIAACPPESQKYLREIRKLIRVLVPDAKERISY
                     QIAAFERNGRNIIHFAGWKKHVSLYPVPAGSEAFERQ"
BASE COUNT          753 a          636 c          727 g          712 t
ORIGIN      
        1 gcgccgcacc cgcacgccat tcgcaccctg cgagagatag ccgaccccat cgtgcatgat
       61 ctcccacggc aaagagagca ggtcactggc tgcttcgcgg atcaacgcgg ctttggcttc
      121 ttctgtgcct tcgagcggtt cgccatccac ctgcactgaa agacgacggc tgccactggt
      181 acgtttccac gcttcgaacg gttcacgagc agattccccg cccaaggcag ccttatataa
      241 cgcattgccc cattctggca gcgccttttc ggttttctcg gcgcgcgttt tgaaaacgcc
      301 cgtgggccac tgataatact tctcgatata ccagcggatc tcgcccaact ccacggctcc
      361 caatggcgca gtgaaacgat agcgtttgct ttcgatggct cgactgttat cagcagggtt
      421 gtaggtcaat acagccgtgg cggttgcgcg gcggatgcct tccttctctt cgatgatcgg
      481 gtcggtcagt tcgagaatca gttcttcaac gggtgcggct ttaataagtg tagggggttc
      541 ccagtcattg ggtaattctt tccccaacgc tgcaaggata tgcggcatct tggaatccag
      601 gtcaggaccc gtcgcgcctt tttctaaaaa tatatggatc ggttctttcg ggaaaaatgg
      661 tttcaacaaa ccagcgggca cgccaggcat gatgatcgaa atgactttat agccttcatc
      721 cttgcgttcc ttggcaacct tgtgggctaa cttcaactct ttttgcaccc actccgaact
      781 aagcgcttca atactcaaaa ggataatgaa agatttggcg gtttttatct gctcttgcag
      841 ggtatcttcg agaatatccc cgcctgtgaa ttcacgcgaa tccacccaaa gctcaaattt
      901 ctcgagttcg agaatttcgc gcaatcgttt gacgacatca tcgttttttg aagagtgcga
      961 gataaagata tgttcctgtc ccatcgaatt gcctcctgcg agaaattcat caatgttcca
     1021 cttggttaag cttggctaac aggtacaaaa ttagagttat gcagcttcca tagtccctta
     1081 ttggaaaatt atacagctaa agatttggat aagtataaac agactcaaat ggcaatatgg
     1141 caaaagtgta acataccgat ctaaaccttg acaaaataga aattatgttc taaaataatt
     1201 tcgcaataag taaccgtttg gttacataaa ggagaccatg agacgagacg tatttcaagc
     1261 tattgcagac cctaaccgta gagcgatcct ctctttgctg gcaaggcaac ggatgacatt
     1321 gaacggtgtt gccgacaatt ttcgtatcag tcgtcctgcc gtttcgaaac acatcaggat
     1381 attgaaagag tgcggactgg tagttgtcat cccacaggga cgtgagcgtt ttgtggaagc
     1441 acggttcgac aagctaaatg aagtgaccga ttgggtcgaa caatatcgcc aattatggga
     1501 aacgcgcttc aatcgattgg atgacctttt agaaatgatg aaacaggaga agaaaaatgc
     1561 cctcgaaaaa taaagtgacc gttaccgctg aacctggaac acaggagctt tttatcgtcc
     1621 gtgaattcga tgcgccgcgt gaattggtgt acaaagccca cattgatcca aaactgtatg
     1681 tgcagtggct tggtccgcat ggatatgaga tggttctcga aacgtttgag cccaccaatg
     1741 gtggaaagta tcgctatgtt cacaaggata aggatggaaa cgaatacggt ttccatgggt
     1801 cgttccatgc gatgtccatc gagaacatga tccagacctt cgagttcgag ggatatccgg
     1861 ggcacgtttc gctggattgt atgacgctgg aggaattgcc cgggaatcgg acgaaagcca
     1921 cgattcattc catctttcaa tctgtctctg accgagacgg catgatccaa aacggaatgg
     1981 aaaaaggtgt gagtgaaggg tatgaacggc tcgatgatat tttgaaggaa atgaagtaaa
     2041 tgaatggata tctggttctt ctgcgcggga tcaatgtggg cggaaagaac atcatcaaaa
     2101 tggccgattt aaaagccagt tttgaggaga tgggcttttc gaaggtggtt acctacattc
     2161 aaagcggaaa tgtcgtcctt cagtcggaag ataaggataa agctgtgctg accaccagaa
     2221 tcgaaaaggg actttcgaag cgcttcaact ttgaggcgaa ggtggtggtg atttctcaaa
     2281 aagaactggc tgctgttgtg aaatctgcac caaagggatt tggcgaggat gacgcgaagt
     2341 ttcgctatga tgtcatcttt ttgaaggagc cgctcacgcc gaaggaagcg atggagagtg
     2401 tgaaagtccg cgagggcgtg gatacggctc atgcaggcaa acaggcgctg tatttttcac
     2461 gtctcatcag ccgcgcttcg cagagttatc tcaccaagat cattggcaca ccggtttatc
     2521 agaatatgac cattcgcaat tggaatacaa ctacgaaact attggcactt atggaaggac
     2581 aagatggcta agacaaattt tcaatccatt gatgagtata ttgcggcatg cccgccagag
     2641 tcgcaaaagt atttgcggga aattcgtaaa ttgatccgcg tgttggtacc ggatgcgaag
     2701 gagaggatca gctatcagat cgcggcgttt gaaaggaacg gcaggaacat catccatttt
     2761 gcggggtgga aaaagcatgt gtcgctgtac ccggttccgg cggggagtga ggcgttcgag
     2821 cgtcagat
//
LOCUS       DAOQLC010000290         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_20879_length_2803_cov_13.183770, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000290 DAOQLC010000000
VERSION     DAOQLC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20879_length_2803_cov_13.183770"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PL000_21830"
     CDS             complement(<1..304)
                     /locus_tag="PL000_21830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC91580.1"
                     /translation="MDAHIQVKDLNAFYGRQQALKNINIEIPKKQITTIMGPSGCGKT
                     TLLKTFNRFFELTENAQITGQVLVDGENIYDKDVDVTEVRKVVGLLAQRPTPLPMSI"
     gene            complement(323..1141)
                     /gene="pstA"
                     /locus_tag="PL000_21835"
     CDS             complement(323..1141)
                     /gene="pstA"
                     /locus_tag="PL000_21835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC91581.1"
                     /translation="MKTIMRIALAIVTGALGLILWAVISRGLPALSWEMVTQVPKGGY
                     YMGKEGGILNAIIGSLYLAIGGTFLAILLCLPIALYLKTYLGDSKWGGYVRLSLDVLW
                     GIPSIVYGAFGFTLMIAFGMRASLLAGIIILALIELPIMARAMDEIIRRMPADLEHAA
                     LALGSTKFEVALQVVTRQMLPGIVTAVLLAFGRGIGDAASVLFTAGYTDRIVTSLMRP
                     TASLPLAVFFQLGSPYPEVQQRGYAAALILTILVLAVSLGSRWLSARLNRYTIK"
     gene            complement(1183..2169)
                     /gene="pstC"
                     /locus_tag="PL000_21840"
     CDS             complement(1183..2169)
                     /gene="pstC"
                     /locus_tag="PL000_21840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNC91582.1"
                     /translation="MSQFHIQINDQTEGRQYRPSAALRARQDQNAKRAFFGITLLPVI
                     LIFIVTIALFVRAWPIMSAYPLSDLLFGKVWKPNNGLFGFWPFILGTVWVTSVGVILS
                     VPACILVAMYLAEYAHNTTRSIAKPILDLLAAIPPVVYGVWGLLAVVPFVDDVLAPLA
                     DRWFASVPIFSVTQPTGFGILSGGIVIAVMIAPLIISVLYEIISTVPNDLRHASLAVG
                     ATQWQTIRRIVLPQVTPGIIAAVVLGASRALGETIAVLMVVGNIPQVPTSVFDSAYPL
                     PALIANNYGEMMSIPLYDAALLGAALVLLVVILIFNILSTIVLQRMLRRKWA"
     gene            complement(2166..>2803)
                     /locus_tag="PL000_21845"
     CDS             complement(2166..>2803)
                     /locus_tag="PL000_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91583.1"
                     /translation="DAVFPIINTENPVAAQILEKGITQEIFNKIYITEEIKTWGEVVG
                     DPTITDEIHVFTRSDASGAADQWAKYTGGEAQEDLKGVGINGEPAMLDTVIKDPLGIG
                     FGNLNSIFDLSSGDLVPGIIIPPIDIDNNGKADAVEIYKVKEDAFGAVANGTYPSPPA
                     RFENLATLGKPEGLTLAFIEWILTDGQQYLEAAGFVPLTPEQQAESLAKLK"
BASE COUNT          664 a          674 c          828 g          637 t
ORIGIN      
        1 aaatggacat gggcagcggt gtggggcgct gtgcaagcag cccgaccacc ttgcgcacct
       61 cggtaacatc cacatccttg tcgtagatgt tttcgccatc cacaagcacc tgacctgtga
      121 tctgggcgtt ttcggtcaac tcaaagaagc ggttgaaggt tttgagcagg gtggttttgc
      181 cgcagccgga aggccccatg attgtggtga tctgtttctt ggggatttca atgttgatat
      241 ttttcagggc ttgttgcctg ccgtaaaaag cgttgagatc ttttacttga atatgggcgt
      301 ccatataaaa tccttttgta tattacttga tggtgtatct gttcaaccgt gccgagagcc
      361 agcgtgaacc gaggctgact gcaagcacga gaatggtcag gatcaatgcg gcggcatatc
      421 cgcgctgctg cacttcaggg tagggcgagc caagctggaa gaaaacagcg aggggcaggg
      481 atgcggtcgg gcgcatcaac gaagtgacaa tgcggtctgt ataacctgcc gtgaacagga
      541 cagaggctgc atcgccaatg ccgcgtccga atgcgagtag cacagcagtg acaatgccgg
      601 gcagcatttg gcgggttacc acttggagtg caacttcaaa tttggtggaa cccagcgcca
      661 gcgcggcatg ttccaggtct gcgggcatgc ggcggatgat ttcatccatg gcgcgggcca
      721 tgatgggtaa ttcgatgagg gcaaggatga tgatgcctgc cagcagggag gcgcgcatcc
      781 caaaggcgat catcaatgtg aatccgaaag cgccgtatac aatggaggga ataccccaca
      841 acacatcaag cgaaaggcgg acgtaaccgc cccattttga atcgccgagg taggttttca
      901 aataaagtgc aattggcagg cagagtagga tggccaaaaa agtgccgcca atggcgagat
      961 ataacgagcc aatgatggca tttaagatgc cgccttcctt gcccatgtaa tatccgcctt
     1021 tgggaacttg tgtcaccatc tcccatgaaa gtgcaggcag gccgcgtgaa atgaccgccc
     1081 ataagatcaa cccgagtgcg cctgtaacga tcgcaagcgc aatccgcatg atggttttca
     1141 tgaatagctc aatgatgtga cgcctttgga atggattttt ctttatgccc attttcgcct
     1201 caacattctt tggagcacaa tggtggagag gatgttaaag atcaggatga cgacaagcag
     1261 gacgagtgcc gccccgagca aagccgcatc atacaacggg atggacatca tttcgccgta
     1321 gttgtttgcg atcagcgccg ggagcgggta tgccgaatcg aacactgatg tgggcacctg
     1381 tggaatattg ccgaccacca tcagtacggc gatggtttca ccgagggcgc gtgatgcgcc
     1441 aagcaccacc gctgcgatga tgcccggtgt cacctgcggc aggacgattc tgcggatggt
     1501 ttgccattga gtggcgccca ctgccagtga agcatgccgc aagtcattgg ggacggtgga
     1561 tatgatctcg tagaggaccg agatgatcaa cggcgcaatc atgaccgcga tgacgattcc
     1621 gcctgagagg attccgaacc ctgttggttg ggtgacactg aagatcggaa cagatgcaaa
     1681 ccaacggtcg gcaagaggcg cgagtacgtc atccacaaat gggaccacgg ccagcaatcc
     1741 ccagacgcca tagactaccg gtgggatggc tgccagcagg tctaaaatgg gtttggcgat
     1801 ggagcgagtg gtgttgtggg cgtattcagc caggtacatg gcaaccagaa tgcaggcagg
     1861 tactgaaaga atgaccccga ctgaagtgac ccagaccgtg ccaaggatga agggccagaa
     1921 gccaaacaga ccgttattgg gtttccatac tttgccaaat aacaggtctg agaggggata
     1981 ggcgctcatg atcggccagg cgcggacgaa caacgcaatc gtgacaataa aaatcagaat
     2041 caccggcaac agggtgatgc cgaaaaatgc ccgttttgca ttctggtctt gacgggcacg
     2101 aagcgcggca gaaggccggt attgccggcc ttctgtttga tcattgattt gaatgtggaa
     2161 ttggctcatt ttagttttgc aagcgattcg gcttgttgtt ccggggtgag cggtacgaaa
     2221 cctgcggctt caaggtattg ctggccatct gtcaaaatcc actcgatgaa ggccagggtc
     2281 aatccttccg gtttgccgag ggtggcgagg ttttcgaacc gtgcgggtgg cgatggatat
     2341 gtgccgttgg caactgcgcc aaatgcatcc tccttgactt tgtaaatttc aacggcatct
     2401 gccttgccgt tgttgtcgat atcgatcggg ggaatgatga tgccggggac gagatctccg
     2461 ctggagaggt cgaagatgct gttcaggttt ccaaaaccga tacccaaagg gtctttgatg
     2521 accgtatcca acatggcggg ttcgccattg atgcccacac ctttgaggtc ttcctgcgcc
     2581 tcgccgcctg tgtatttagc ccactggtct gcagcgccgg atgcatcgga acgggtgaag
     2641 acgtggattt catcggtgat ggtggggtcg ccgaccactt ctccccaggt tttgatctct
     2701 tcagtgatat agatcttgtt gaagatttcc tgtgtaatac ctttttcaag aatttgggcg
     2761 gccaccgggt tttcggtatt aatgatcggg aagaccgcat ctt
//
LOCUS       DAOQLC010000291         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_21052_length_2790_cov_16.426691, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000291 DAOQLC010000000
VERSION     DAOQLC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21052_length_2790_cov_16.426691"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="PL000_21850"
     CDS             complement(<1..1974)
                     /locus_tag="PL000_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HNC91584.1"
                     /translation="MMAKKTRHEVLDSIAALRVSHSLDLFPHVIKKLNQRRDLMQALR
                     TRPILVVALFVVVILLLNGVAYAVDRFVGYIPGFGFTSGSQAVLVLDAPVSQTHNGIT
                     LRVDQAISDGDRFWVEMTVSGYSFFETQDHRDAYPNPDTGEYPPAFLVLPSGEKIQSL
                     FGESPNLGAEEILFRYEFPPLKETPPLLTLVVEKMGGETFVMPLKMRPIHQSEIVPAQ
                     PEENTPVKSVTHDGLTFVMEKVAQSSTETFFEFTLQADDPRLSLLSPWSVLVTDQHGN
                     IYPLITHTKASVYLEGAKTYKSAPFHGDEQLTMRLMGEIFTRGKLPAQYEYLIDELSP
                     SFHFDPGSAPQVGQTWQLDETLTGNGFTLHLVGARVASEHEIIFEIEHSTNVTRIAFR
                     AEPPAQTWADVPPDGGNIQLRVGYRSLPTAPYELSLSTVMFMLEENWEIKWIPQPAPQ
                     PSGDALLSTPTPGTPPEIHATPTLTSTAPFLLEMEALSQKFNAQFKTGPAWIHVVWEG
                     GQYQTPGLLNYPPAYYTIEKWMEIDKDGLIIRELNTDRDVDEMILQQNVSMNGYGVNL
                     TQGNGGAMSSNTLYQFTEDYIVEVLDRAYQNGQSVKHEETTCEGRPCWLIWIQSDVET
                     FRHPVIQKMVVGNIQKIWMNFQTGRLIRSESIWQ"
     gene            complement(1964..2521)
                     /locus_tag="PL000_21855"
     CDS             complement(1964..2521)
                     /locus_tag="PL000_21855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC91585.1"
                     /translation="MDDLQAVRRIKEGDWSGLEVLMERYQVRAVRAAFLILHDESAAQ
                     DVVQDTFIRICRRIHQFNEEHAFEPYFFGSVIHAALNATRGNHKFVSLDEKSEEIEHL
                     LDCAVSAESQVEFLQFQHEILTALSELSPRQRAAIVQRYYLEMSEKEMAFAMQAAPGT
                     IKWLLNAARDRLRRLLGPKGESNDG"
BASE COUNT          757 a          670 c          623 g          740 t
ORIGIN      
        1 ttgccaaata ctttcagagc gaataagcct cccagtttga aagttcatcc aaatcttttg
       61 gatattgccc acaaccattt tttgtatgac gggatgacga aatgtttcca catcagactg
      121 aatccagatc aaccagcaag gtcgtccttc acaggtggtt tcctcatgtt ttacagattg
      181 tccattttga taggcacggt caagaacctc aacaatgtaa tcttctgtaa attgatacag
      241 agtattggaa ctcatcgcgc cgccattccc ctgtgtaaga ttgactccat aaccattcat
      301 actgacattt tgttggagga tcatctcatc aacatccctg tcggtgttta gttcacgaat
      361 gatcaaaccg tctttatcga tctccatcca tttctcaatt gtgtaatatg cgggcgggta
      421 gtttagcagc ccaggggttt gatattgccc accttcccaa acaacatgaa tccatgcagg
      481 gccagtttta aattgggcgt tgaatttttg agagagggct tccatttcca aaaggaatgg
      541 ggcggttgag gtcaatgtgg gggtggcgtg gatttcagga ggagtgccag gcgttggggt
      601 ggagaggagc gcatcacctg atggttgagg ggctggttgt ggaatccatt ttatttccca
      661 gttttcttcc aacataaaca tcacagttga aaggctgagt tcatagggtg cagttggcaa
      721 agatcggtac cccacgcgaa gctggatatt ccctccatct ggtggtacat cggcccaggt
      781 ttgagcaggt ggttccgccc gaaaagcaat acgagtcaca tttgtggagt gttcgatttc
      841 aaaaatgatt tcatgctctg atgccacgcg cgcgccgact aaatgtaaag taaagccatt
      901 gccggtcagg gtttcatcaa gttgccaggt ttgtccgacc tgtggagcgg agccagggtc
      961 aaaatgaaat gagggtgata gttcatcaat cagatattcg tattgagcgg gtaattttcc
     1021 acgggtaaag atctcgccca tcaaacgcat ggtcaattgt tcatcaccgt gaaatggcgc
     1081 agatttataa gtctttgcac cctccaggta caccgaagcc ttggtatgtg ttatcagcgg
     1141 atagatattt ccatgctggt cagtgaccag aacgctccat ggagaaagca gactcaagcg
     1201 tggatcatca gcctgcaagg taaattcaaa aaaagtttca gtgctggatt gcgccacctt
     1261 ttccatgaca aatgtcagcc catcatgggt tacgctttta acaggggtgt tctcttcagg
     1321 ctgtgcaggg acgatctcgc tctgatggat cggacgcatt ttcaacggca tgacaaaggt
     1381 ttcaccgccc atcttttcca cgaccaatgt caacaaggga ggtgtttctt tcaaaggcgg
     1441 aaattcatat cggaaaagaa tttcttcggc ccccaaattt gggctttctc caaacaggct
     1501 ttggattttt tctccgcttg gaagcacaag aaacgcaggc gggtattctc ctgtatcggg
     1561 atttggatag gcgtcccgat gatcttgtgt ctcgaaaaac gaatacccgc tcacggtcat
     1621 ctcaacccag aacctgtcac catcgctgat agcttggtcc acccgtaaag taatgccatt
     1681 gtgggtttgc gacacaggcg catccaatac caaaacagct tgtgagccag aagtgaatcc
     1741 aaaacctgga atatacccaa cgaaacggtc aacggcatag gcaacaccat tcaacaatag
     1801 tatgactact acaaacaggg ctactacaag aatcggacga gtgcgcaaag cttgcatcag
     1861 atccctcctt tgattcaact ttttaataac gtgtggaaag agatcaaggc tgtgcgaaac
     1921 acgcagagcg gcgattgaat ccaatacttc atgacgagtc tttttagcca tcatttgact
     1981 cacctttcgg cccaagcagg cggcgtaatc tgtcacgggc tgcgttcaat agccatttga
     2041 tcgttcctgg ggcggcctgc atggcaaaag ccatttcctt ctcgctcatc tccaaatagt
     2101 atcgttggac aattgctgcc cgctggcgag gagaaagctc tgacaatgca gtcaaaatct
     2161 catgctgaaa ttgcaaaaac tccacttgcg attccgctga aactgcacag tcgagcaaat
     2221 gctcgatttc ttctgatttt tcgtccaggg atacaaattt atgatttccc cgtgtggcat
     2281 tcaaagctgc atgaatcaca cttccaaaaa aataaggctc aaaggcatgt tcttcgttaa
     2341 attggtgaat ccgcctacag atgcggataa atgtgtcctg aaccacatcc tgtgcggcag
     2401 attcatcatg cagaatcaga aatgcggccc gcaccgctcg tacctgatag cgttccatca
     2461 aaacttccaa cccgctccaa tcgccttcct taatgcggcg aacggcctgc aaatcatcca
     2521 tgaagttttc ccttgtcctt tttgctccca tatcaggtat acacaatcat aaacgaaaag
     2581 gttgggtcct caacacctaa atcctccagt tcaatttcca gctaaattca tacagcatat
     2641 catcttgaca tataaataca aattgataaa ataaccgaat ataaattcag ttattttgag
     2701 gaagcatgcc caagcccgat gtcagcgccg agcgaattcc gctcatacta aattccgcca
     2761 tcaaggtctt ttccaaaaaa ggctttgaag
//
LOCUS       DAOQLC010000292         2782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_21180_length_2782_cov_21.222589, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000292 DAOQLC010000000
VERSION     DAOQLC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21180_length_2782_cov_21.222589"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2725
                     /locus_tag="PL000_21860"
     CDS             179..2725
                     /locus_tag="PL000_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91586.1"
                     /translation="MARKKPASQKAGYAGCIDAIRQSAGAWLSDKDAEVLLEEIDKVV
                     QRNRARGKLESIEDQVLNAIDGATNAMKEAALIEKRNALINARIKEQVHGYLDGFQDK
                     AEGLLAYLGGGVKLRQGGKLSIDAREKSIVNGILGKLINSLEQEKLLPFFTSGELDDD
                     IARELWALGEGRAGITKNQEALGIAEIINAAQTELVGMQNRAGAHIRMMPGYIVRQSH
                     DVGRIRAAGQEAWTQFVLPLLNEEKTFGNMEVAPFLEEAYMGFSTGLHMKQRGEEVAD
                     DAAALLGFTGPGNLAKKISANRVLHFKDADSWLKYNEAFGQGSLRESILWGVESASKN
                     LALMEGLGTNPESMFKTLIGELKEASRNDPEAFDKLNNWSLEALYRQIDGSARIPANR
                     TLAAWGSGIRVLQNMAKLGFAVLSSVTDIPFQAAALKYNGVSLFEAYINAFANVLRGR
                     GDADRKEIARLVGSGFQGILSDIASRFAAEDNIPGLMAKLQQKFFKLNLMNWWNDAHK
                     SGVAVMLSNHLGGLKDMDFSALPDATKRMFKLYDINEREWNAIRSTAWERDGDWYLTP
                     DRIAGLDESRMKEVYSLNNLDISSESFRELRKLRDSLEVKFRAYLTNQIESAIPHPDA
                     RVQSLLNLGTQAGTPLGEAVRFIMQFKSFPIAVLRRGVAPFVYGNGAESFSEALFKGK
                     ADYAGLAHIMVASTVFGYLAMVAKDTVRGRTPKDPTDPKTIIAAMTQGGGLGIYGDFV
                     FGEFNKYGHSAIATLSGPTVGQFEDLARLYSRAKEGDSVAGQSLRVMMNNTPFANLFY
                     TRAALDYLFLHNLQETVNPGYLSRMEQRIQRESGQHYYLPPTQVIPYGGR"
BASE COUNT          687 a          648 c          793 g          654 t
ORIGIN      
        1 aagactcgca agatgatgga gttggaaaag gagatagagt accaacaatt acgtcttgcg
       61 caagagcacg gggacgttca ggttgatgga ctcaaacagg cagacgatgc tttgagtaag
      121 gcagatgact tgcgcgccgc catggttgaa ggcatggctt gtttgttaag gagataacat
      181 ggcacggaaa aaacctgcat ctcaaaaagc tggctatgct ggctgtattg atgctatccg
      241 tcagtcggcc ggtgcgtggc tatctgataa ggacgcagaa gtcctgctgg aagagattga
      301 caaggtggtt cagcgcaaca gggctagggg caaactcgag tccatcgagg accaggtact
      361 aaacgctatc gacggcgcga ctaatgctat gaaggaagcg gccctgattg aaaagcgtaa
      421 tgccctcata aacgctcgga tcaaggagca ggtgcatggc tacctggatg gttttcagga
      481 taaggctgaa ggactgcttg cgtatctcgg tggtggtgtt aaactccgcc agggaggcaa
      541 actatcaatc gatgctcgtg aaaagtcaat cgttaacggt atcctcggca aactaatcaa
      601 cagtctcgaa caggagaaac tgttaccatt cttcacatca ggtgagcttg atgacgacat
      661 cgccagggaa ttgtgggctc taggtgaagg gcgcgccggt atcacgaaaa accaagaagc
      721 ccttggaatc gctgaaatca ttaacgcggc gcaaacggaa ctggttggca tgcagaaccg
      781 ggctggggcg catattcgca tgatgcccgg ctatattgtc cgccagtcac atgatgtggg
      841 tcgaattcgg gccgctggcc aagaagcctg gacacaattc gtattgccct tgctgaatga
      901 ggaaaagacg ttcggcaaca tggaggtagc tccgtttctg gaagaggcct acatgggttt
      961 ctcaaccggt ctgcacatga agcagcgtgg cgaggaagtt gctgatgacg cagcagcctt
     1021 gcttggtttt actggacccg gcaacctggc caagaagatc agcgctaacc gggttctcca
     1081 cttcaaggat gcggactcgt ggctgaagta taatgaggct ttcgggcaag gcagccttcg
     1141 tgagtccatc ttgtggggtg tagaatccgc ctcgaagaat ttggctttga tggaaggcct
     1201 tggcacgaac cccgagtcga tgttcaagac acttattggc gaattgaagg aggcttcacg
     1261 caacgatcct gaggcgtttg acaagctgaa taactggtcg cttgaggccc tgtataggca
     1321 gattgatggc tctgcgcgta ttccggctaa ccgcacacta gccgcgtggg gctcaggtat
     1381 tcgcgtgctt cagaacatgg cgaagttggg ttttgcagtg ctgtcatcag tcacagatat
     1441 cccattccaa gcggctgccc tgaagtacaa tggcgtaagt ctgtttgagg cctatattaa
     1501 tgcattcgct aacgtgctcc gtggccgtgg cgatgctgat cgaaaagaga ttgcccgctt
     1561 agtaggctca ggcttccaag gcattctgtc tgacattgcc agtcgctttg cggcagagga
     1621 taacattccg gggctgatgg ccaagctgca acagaagttc ttcaaattga atctgatgaa
     1681 ctggtggaac gacgcacaca agtctggtgt ggctgtgatg ctgtcaaacc atctcggtgg
     1741 cctgaaagac atggacttca gtgcactgcc agatgccacc aagcggatgt tcaagctcta
     1801 cgacatcaac gaacgtgaat ggaatgctat tcgttctacg gcctgggagc gtgacggcga
     1861 ctggtatctt acgcctgacc ggattgctgg attagacgag tctcggatga aagaggtcta
     1921 tagtcttaat aacctagata tatcctccga gtccttccgt gaactaagga agctaaggga
     1981 tagcctagaa gttaaattta gggcctattt gaccaatcag attgaaagtg ccatcccaca
     2041 tccggatgct cgcgtccaaa gtctcctaaa ccttggaacc caggcaggta caccgctcgg
     2101 tgaggccgtt cgcttcatca tgcagttcaa gtcattcccg attgcagttc ttcgtcgtgg
     2161 tgtggcgccg ttcgtttatg gcaatggggc cgaatcgttc agtgaggcat tattcaaggg
     2221 caaagcggac tacgctggat tggcccatat catggttgcc tcgaccgtgt tcggttacct
     2281 ggcgatggtt gctaaggaca cggttagagg acgtactccg aaggatccca ctgatccaaa
     2341 gacgatcatt gcggccatga cgcaaggtgg tggcttgggt atttacggcg acttcgtgtt
     2401 tggtgagttc aataagtacg ggcattcagc aatcgctacg ctatctgggc caactgttgg
     2461 acagttcgag gatcttgcgc ggctgtactc tcgcgccaag gagggagact ctgttgctgg
     2521 tcagtctttg cgagtcatga tgaataacac accgttcgcg aatctgtttt atacgcgtgc
     2581 tgcgttggac tatctgttcc tgcacaacct acaggagacg gttaaccctg gctatttgag
     2641 ccgcatggag cagcgaattc aacgcgagag cgggcagcat tactatctgc caccgacaca
     2701 ggttattccg tacggtggca gataaattcc gagcactagt gctaataacc atggtacaat
     2761 ctataataac atcggagtaa ag
//
LOCUS       DAOQLC010000293         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_22029_length_2723_cov_16.393553, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000293 DAOQLC010000000
VERSION     DAOQLC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22029_length_2723_cov_16.393553"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PL000_21865"
     CDS             <1..498
                     /locus_tag="PL000_21865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNC91587.1"
                     /translation="AKLKPAFKEGGFVTAGNSSQMSDGASAVMVMSADKAAGLGLKPL
                     ARFVAFAVGGVAPELMGIGPVAAIPKVLKTAGLSLNDIDLFELNEAFAAQSLAVMQTL
                     EMDPEKVNVNGGAIALGHPLGCTGSKLTTQLIYEMGRRKSKYGMVTMCIGGGMGAAAI
                     FENLQ"
     gene            578..904
                     /locus_tag="PL000_21870"
     CDS             578..904
                     /locus_tag="PL000_21870"
                     /inference="COORDINATES: protein motif:HMM:NF026214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNC91588.1"
                     /translation="MALTIETLMSKMPGAFIPEKAAGLDATIQFKFTGEEPGEWYATI
                     KDGKCEVAKGTHASPKMTLTADSSDYVKIFTGEIDGMQAFMQGKLKLAGDLNLAMKLT
                     QMFKIK"
     gene            975..1775
                     /locus_tag="PL000_21875"
     CDS             975..1775
                     /locus_tag="PL000_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91589.1"
                     /translation="MNLNWSGFGIYGCVCGFSLIALVIFVVIANIVNKPAVDQINAKH
                     DERIKRKIVIYENGGLLAPGIIISAIRVRSWGDTSKRRNHKFHLIDYEVDVFPPNDAP
                     FHTKFREEIVREGYQIKDGQMVSEHGWKIWVTYDPSDKSKAFLDHFDAEHESVMETRD
                     LDFRQMKYNLIFEENEKIERYGQQAEAVITQIDDLQLPASLLKSRAMHLHLNVNTGAG
                     ANRQAECDAMISEAFLSKYTVGRKVFIRFDSQNPRRVALDTKRNKKLE"
     gene            1928..2707
                     /locus_tag="PL000_21880"
     CDS             1928..2707
                     /locus_tag="PL000_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91590.1"
                     /translation="MAENSTWTIIIVLGCVTVLGLIVVAPGFVFARLISTSASRNIEK
                     ERDNIRKHKQSVRVSGGVTAPAVITSAKKTGMTEASFVDFEAEVKPADNPSFLVKFRD
                     ELYGDEYDVVGYDVVSEIGRKISVTYDPRDKTMAFLDHYDDEYEVSIKKMELIERRVE
                     FDKLISGNEELKIRGEQAEAVITRVDDLKLSYPQKKSQAMHLCFDVIPKNGPVFKAEG
                     NALIVTTSLDKYSVGKRIYVRYDPQQPEKAVLDTARNKILK"
BASE COUNT          772 a          570 c          662 g          719 t
ORIGIN      
        1 gccaaactca agcctgcttt caaggaaggc ggttttgtca ccgctggaaa ttcctctcaa
       61 atgtcggatg gcgcttcggc agtaatggtc atgtccgcgg acaaggctgc tggattagga
      121 ctcaaacctt tggcgcgctt cgtcgccttc gccgtgggcg gggttgcccc cgaattgatg
      181 ggcatcggtc ccgtagccgc cattccgaag gtcttgaaga cggcgggatt atccctcaac
      241 gatattgacc ttttcgaact gaacgaagcc tttgcggctc aatctctcgc cgtgatgcaa
      301 accctcgaaa tggatcctga gaaggtcaac gtcaacggcg gcgcgatcgc cctcggacat
      361 ccgctcggct gcacaggctc caagctcacc acccaactca tctacgaaat gggacgccgc
      421 aagtccaagt atggcatggt caccatgtgc atcggcggcg gtatgggagc ggcggcgatc
      481 ttcgagaatt tacaataaaa gttacaggtt gcaggttgaa tgttcaaagg ttcaacttgt
      541 aaccttcaac cttcaacttt ctaacctagg agaaacaatg gctctcacaa tcgaaaccct
      601 catgtccaaa atgcctggtg cattcattcc tgaaaaagca gctggattag atgcaaccat
      661 tcaatttaag ttcactggcg aagaacctgg cgagtggtat gccaccatca aagacggcaa
      721 atgcgaagtt gcgaagggaa cacacgcttc cccgaaaatg accctcaccg ctgattccag
      781 tgattacgtc aaaattttca ctggtgagat cgacggcatg caggctttta tgcaaggtaa
      841 gctcaaactc gctggtgatc tgaatctggc aatgaaactg acccagatgt tcaagatcaa
      901 gtaattcgaa aagtcttgct gtaaaataca tccaatccag gcgattggat gtattttatt
      961 atcaaaggtg ttttatgaat ctgaattggt ctggctttgg aatttatggt tgcgtttgtg
     1021 gattctcttt gatcgcattg gtgatttttg tggtcattgc caacattgtc aataaaccag
     1081 cggttgatca gatcaatgca aagcatgatg agaggataaa acgtaagata gtcatctatg
     1141 aaaatggcgg cctccttgct ccgggcatca tcatttccgc aatcagggtg agatcatggg
     1201 gcgataccag caaacgccgg aaccataaat ttcatctaat tgattatgag gtggatgtct
     1261 ttccgcctaa tgatgcgcct ttccacacta aatttcgcga ggaaattgtt cgggaaggtt
     1321 atcaaattaa ggatggccaa atggtctccg agcatggctg gaagatctgg gtgacttatg
     1381 atcccagtga caaatccaaa gcattccttg accattttga tgcagaacac gaatcggtaa
     1441 tggaaactcg agacttggat tttcgacaaa tgaaatacaa ccttattttt gaagagaatg
     1501 aaaaaattga aagatatggt cagcaagccg aggcggtcat cacccaaatc gatgacctgc
     1561 aattacctgc ctcgttgctc aaatcacgtg ccatgcatct ccaccttaat gtaaatactg
     1621 gggcaggtgc aaaccgccag gcagaatgtg atgccatgat ctccgaggct ttcctttcaa
     1681 aatacactgt gggaaggaaa gtatttatcc gctttgattc gcagaatccc aggcgggttg
     1741 cgcttgatac gaaacggaac aagaagctgg aataaccctg tatgcttctt gtttctgaga
     1801 gaacttaact ggttgataat ctagctctat tgccgtaatt acctcagatg ttcaagtttg
     1861 ggtaaaaaag attttgttgt aaaatacgtc caattactct gtttgggcgt atttttattg
     1921 gagaaaaatg gcggaaaatt ctacttggac aataattatt gttttagggt gcgtcaccgt
     1981 acttggtttg attgttgtag caccgggctt tgtttttgct cgcttgatca gcactagtgc
     2041 aagcaggaac attgagaagg aaagagacaa catcagaaaa cataagcagt ctgttcgtgt
     2101 aagcggaggg gtgacagctc ccgcagtaat tacatctgcc aaaaaaacgg ggatgacaga
     2161 ggccagtttt gtagattttg aagcggaagt taaacctgct gacaatcctt ccttccttgt
     2221 gaaatttcga gatgaattat atggagatga gtatgatgtt gttgggtatg atgtagtctc
     2281 cgaaatcgga cgaaaaattt cagtcactta tgatccaaga gataaaacca tggcgttttt
     2341 ggatcactat gatgatgagt acgaagtgtc cataaaaaag atggagttga ttgaacgccg
     2401 tgtggagttt gacaaattga tcagcggtaa tgaagagctt aagatacggg gcgagcaagc
     2461 cgaagctgtg ataacccggg ttgacgactt aaaactgtca tatccacaga agaaatccca
     2521 ggcaatgcat ttgtgcttcg atgttatccc gaaaaatggg cctgttttta aggcagaagg
     2581 taatgccttg attgtgacaa cttcactgga caaatattcg gtgggtaaaa ggatatatgt
     2641 ccgttatgat ccgcaacaac cggaaaaagc tgtgttggat accgcacgga ataaaattct
     2701 taagtaagca tgctcttgtt gag
//
LOCUS       DAOQLC010000294         2696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_22431_length_2696_cov_15.766376, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000294 DAOQLC010000000
VERSION     DAOQLC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22431_length_2696_cov_15.766376"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PL000_21885"
     CDS             <1..279
                     /locus_tag="PL000_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91591.1"
                     /translation="IDKAKIELSEYVGEITRLEKELEQSQNITAETGKRLSTAMTQIS
                     ELATQKTALETRLSDRDNELDRMRADYKKLQTELITIAKTKAKQNKVG"
     gene            276..494
                     /locus_tag="PL000_21890"
     CDS             276..494
                     /locus_tag="PL000_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91592.1"
                     /translation="MSPQYLHLMLVPCNSLKGNAGMDWGLYKYRHLVENAFARLKQYR
                     AVATRYDKLKRNFKSMVVMACGYLWLPM"
     gene            complement(798..1868)
                     /locus_tag="PL000_21895"
     CDS             complement(798..1868)
                     /locus_tag="PL000_21895"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNC91593.1"
                     /translation="MITFTQGNLLEARAEALVNTVNTVGVMGKGIALMFKERFADNMR
                     LYAIACKAKQVQTGKMFVTEVHELDGPRWIVNFPTKQHWKAPSQMAWVEEGLQDLRRF
                     LIEKQVKSIAIPPLGAGNGGLEWTEVREQIEIALGDLPNIEIVVFEPTQQYQNVAKCA
                     GVGRLTPARALIAELVRRYWILGMECSLLEIQKLAWFLERAIEQFSPEDNPLKLQFVA
                     HKYGPYANKLDHLLNNLDGSYLHCDKRISDAGPLDIIWFDDEHKALVQTYIKTEAKAY
                     EQALEYTAALIDGFESPFGMELLATVDWLLSKEGVQPTVQAVREGLRQWPAGATERKT
                     RLFDDRAIGIALDRLTAHIILD"
     gene            complement(1868..2512)
                     /locus_tag="PL000_21900"
     CDS             complement(1868..2512)
                     /locus_tag="PL000_21900"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HNC91594.1"
                     /translation="MIYTNLNPEKALIWRIVHRDNLPWILDNGLHCASSPTQASAYVA
                     IGNTDLISRRTYREVPIEPGGTLADYVPFYFTPFSPMMYNIYTGRGEVPKRSNEEICI
                     LVSSLSRVNVMGLRFVFTDRHAYSALARYFNDTALLNEIDWTLLQARNFKRNPDDPAQ
                     IERYQAEALVYGHLPTAALLGVVCYTEAVKLMLESQVQARGLTLDIKAIPGWYF"
BASE COUNT          759 a          686 c          586 g          665 t
ORIGIN      
        1 atagataaag ccaagattga gttatcagaa tatgtgggag aaattacgcg cctggaaaaa
       61 gaattagaac aaagccaaaa tatcactgca gaaaccggaa agcgcctttc caccgctatg
      121 actcagattt cagaactggc cactcaaaag actgctctcg aaacgcgcct ttcagaccgt
      181 gataacgaac tggatcgcat gcgagctgat tataagaaac tacagaccga gcttattaca
      241 atagccaaaa ccaaggccaa acagaacaag gtgggataag cccacagtac ctgcacttaa
      301 tgctcgttcc ttgcaattca ttgaaaggta atgcaggcat ggattggggc ttgtataaat
      361 atcggcattt ggtagaaaac gcttttgcgc gattaaagca gtatcgcgca gtggcaacac
      421 gatatgacaa attgaaaaga aattttaaga gtatggtagt catggcttgt ggatatctgt
      481 ggctacctat gtgaaatgtc aacagaccct aggagtctgt cggataggca ttgtttcgat
      541 cgctcgaatg gataattact ggcatattga atgataaata gtgattaaag tggtttttta
      601 atttcattaa atggcatttt acatgaatcc ggtgggattg atatggtgtc tattcgctaa
      661 gtccgacaga ctcctagtag ccagattcat gtaattttgc acgcaaagaa tttattttct
      721 cctcactatt ccctcaacaa tttaatcaac ccacgtaaaa aacggttcaa taacatgttg
      781 aatctgcaaa ccgtaaatca gtccaaaata atatgtgcag tcaggcgatc caacgcaatg
      841 ccaattgccc tatcatcaaa caaacgagtc ttacgctcgg tggcacccgc aggccattgc
      901 cgtaaccctt cgcgtacagc ctgaaccgta ggttgtacgc cttctttact cagcaaccaa
      961 tcgacagtag ccagaagctc catgccgaaa ggagactcaa agccgtcgat caaggcggcg
     1021 gtgtattcca gcgcttgctc ataagccttg gcctcggtct tgatataagt ttgcaccaaa
     1081 gccttgtgct catcgtcgaa ccaaattata tccagcggcc cagcgtcact gatacgctta
     1141 tcgcaatgca gatagctgcc atccagattg ttgagaagat gatcgagctt attggcataa
     1201 ggcccgtact tgtgtgcaac aaattgcagc ttgagcggat tgtcctcagg agaaaactgc
     1261 tcaatggcac gctccagaaa ccacgccaat ttctgaatct ccagcagact gcactccatg
     1321 ccaagtatcc agtagcggcg cactagttca gcaatcagcg cgcgagccgg tgttagcctc
     1381 cccactccag cgcatttggc tacgttctga tactgctggg tgggctcgaa caccacaatc
     1441 tcgatatttg gcaagtcacc cagagcgatc tcaatttgtt cgcgtacctc agtccattcc
     1501 aagccgccgt tccctgcgcc caaaggtggg atggcaatag atttaacctg cttttcgata
     1561 agaaaacggc gcaaatcctg caagccttcc tccacccatg ccatctgtga aggcgctttc
     1621 caatgctgct tggtggggaa attcacgatc cagcgcggac cgtcgagttc atgtacctcg
     1681 gtgacaaaca tcttgccggt ctgcacttgc ttggctttgc atgcgatagc ataaaggcgc
     1741 atgttatcgg caaagcgctc cttgaacatc aacgcgatgc ctttgcccat taccccaacg
     1801 gtgttgaccg tgttcaccag cgcctcggca cgggcttcaa gaaggttgcc ttgtgtaaac
     1861 gtaatcatca gaaataccac cccggtatgg ctttaatatc cagcgttaaa cctctggcct
     1921 gcacttgact ctcaagcatt aattttactg cctccgtata acaaacgaca cccagcaacg
     1981 ccgcagtagg cagatgccca taaaccagcg cttctgcttg ataacgctcg atctgtgccg
     2041 ggtcatccgg gtttcgctta aaatttcttg cctgcagcaa tgtccagtcg atctcgttca
     2101 acagtgccgt atcattaaaa tatcttgcca aagcagaata cgcatgacga tcagtaaata
     2161 caaaacgcaa tcccatgacg ttcacacgcg acaagctgga gaccaatata cagatttctt
     2221 cattactacg cttaggcacc tcgcctcgcc ccgtgtatat gttgtacatc ataggcgaaa
     2281 acggcgtgaa ataaaatggt acgtagtctg ccaaagtgcc gccaggctca attggaactt
     2341 ctcggtatgt acgccgagag ataaggtctg tattcccaat tgctacgtag gcggatgcct
     2401 gcgttggcga gctcgcacaa tgcagcccat tatccagaat ccaaggcaga ttgtcgcgat
     2461 ggacgatgcg ccagatcagc gctttttctg gattaagatt cgtgtaaatc atgataaaaa
     2521 cattcgctcg ttttacctta acttagggta ctgtgcatct tcatccgtaa atatacgcta
     2581 ggacagtacc ctttatatgg gtattgcaga agaaccccac ttaataaccc caagtaaaaa
     2641 ccggctcaac cacatgttga atctgcaaac ccgcaatcag cccaaaatac cgcgca
//
LOCUS       DAOQLC010000295         2669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_22853_length_2669_cov_21.489671, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000295 DAOQLC010000000
VERSION     DAOQLC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22853_length_2669_cov_21.489671"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PL000_21905"
     CDS             <1..233
                     /locus_tag="PL000_21905"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91595.1"
                     /translation="MAIFLLRGEHGSAYTPPAGTGAVFGDVPAGSFAVDWIEQLAAEG
                     ITSGCGGGNYCPNANVTRAEMAVFLVRAFHLP"
     gene            317..>2669
                     /locus_tag="PL000_21910"
     CDS             317..>2669
                     /locus_tag="PL000_21910"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91596.1"
                     /translation="MKYKTSRFVHLFFVLILFFNLAGAPAPEMVQPAVTTSNPILFVT
                     QLPIRQDFTTITAVFGNHKAGMQDVGRGGDLWIRYGDGSLKNLTQAAGYGSTGANGFQ
                     DHNAISVRDPYVYWDGTKALFSMVIGAPEQQYTVETYYWQIYEITGLGKNQSPVITKV
                     PNQPEGFNNITPIYGTDDRIIFTSDRPRDGSLHLYPQLDEYEEAPTVSGLWSLDPATG
                     DLFMMNHAPSGDFTPIIDSYGRVIFTQWDHLQRDQQADADKYDGGTYGTFNWSDESAG
                     STPLNIRTEIFPEPRADEEAAGTNLMQHTFNMFMPWQINEDGTEPETINHIGRHELAH
                     YISNSFTDDPNVVYNTPGPIFNPNKADIFLQIKESPTKPGTYFGATMPEFGTHGSGQI
                     ISMTAPPGLDADHISITHITHPDTSSTTATANNSGHYRDPLPLSDGQIIAAHTTQTGG
                     DASSGASIYDFRLKTLAVGGNGYYTASQALTSGISETISYWSPDTKVTFSGVLWELQP
                     VEVRARTRPAKLTATLEATEQAVFSQAGVNVADFQAYMQANNLALIVSHNVTTRDHSD
                     VQQPLNLRVPGGAETIFASGKVYDVQYLQIFQADQLRGIGGTQSPRPGRRVLAQNLHD
                     PAVAFVQPPNPDGPVGSVVLGLDGSMAAFVPARRALTWQLTDPSGVGVVRERVWVTLQ
                     PGEIRVCKSCHGVNTADQAGNPPPDNEPQALLNLLEYWKVAANSLFADVPASHWAASF
                     IERLYNAGITGGCGTAPLLYCPEATVTRAQMAIFILRGMHGSAY"
BASE COUNT          622 a          699 c          692 g          656 t
ORIGIN      
        1 aaatggcgat cttcctgttg cgtggcgaac atggcagtgc ctacaccccg cctgcaggga
       61 caggtgcagt ctttggggat gtacccgcag gctcctttgc tgtggattgg atcgaacaac
      121 tcgcagccga aggcatcacc agcggctgcg gcggcggaaa ttactgcccc aatgccaatg
      181 tcacccgtgc agaaatggct gtctttttag tgagggcatt tcatttaccg taaacgagtt
      241 tgctaaaaat caaaaagtgc ctttgtgtat tgaggcactt tttgatttcc aaaatattgt
      301 catggagaat ttaataatga aatataaaac atcgcggttc gttcatcttt tttttgtatt
      361 aattctgttt ttcaaccttg caggagcgcc tgcgcctgag atggttcaac ctgcagtgac
      421 cacctccaac cccattttgt ttgtaacgca gctcccgatc cgccaggatt ttacgaccat
      481 caccgccgta tttggcaacc ataaagcagg gatgcaggat gtggggcgcg gcggcgatct
      541 atggatccgt tatggcgatg gttcacttaa gaatctgact caggctgccg ggtatggcag
      601 cacaggtgcc aatggatttc aggaccataa cgcaatttct gtgcgcgatc cgtatgtgta
      661 ttgggatggt accaaggcgc tttttagcat ggtcatcggc gctccggagc agcaatatac
      721 tgtggaaacc tattactggc agatctatga aatcaccggg ttgggaaaga accaatcccc
      781 tgtgatcacc aaagttccaa accagcctga agggttcaac aatattacac cgatctacgg
      841 cacggatgac cgcatcattt tcacctctga tcgtccgcgt gatggttcgt tgcatttgta
      901 tccgcagttg gatgaatatg aggaagcgcc tactgtcagc gggttgtgga gccttgaccc
      961 tgccactggt gacctgttca tgatgaacca tgccccatcc ggtgatttca ctcccatcat
     1021 cgatagctat ggccgtgtca tctttacgca gtgggatcat cttcaacggg atcaacaggc
     1081 tgatgcggat aaatacgatg gcggtacata cggaaccttc aactggtcgg atgaatcggc
     1141 aggctccacg ccgctcaaca ttcgcaccga aatctttcca gagccgcgtg cggatgaaga
     1201 agcagcaggg acgaacctga tgcagcacac attcaatatg tttatgccct ggcagatcaa
     1261 tgaagatggc accgaacccg aaaccatcaa tcacattggc cgccatgaac tggcacatta
     1321 catttccaat tcatttacag atgatcccaa tgtggtttac aacacgcctg gcccgatctt
     1381 taaccccaat aaggcggata ttttcctcca aattaaagaa tcgccgacaa agccggggac
     1441 gtatttcggc gcgaccatgc cagaattcgg tacgcatggc tccgggcaga tcatcagtat
     1501 gacagcccct cccggattgg atgccgacca tatttccatt acccatatca cccatccaga
     1561 tacatccagc accactgcaa cggccaacaa ttccggtcac taccgcgacc cgttgccgct
     1621 ttcggatggt cagatcattg ccgcccatac cactcagacc gggggcgatg catcttctgg
     1681 agcgtcgatt tatgacttcc gcttaaaaac cctcgccgtg ggtggcaatg gatattacac
     1741 cgcaagtcag gcattgacct ctgggatttc ggaaaccatc agttattgga gccccgatac
     1801 aaaagtgacc ttttccggtg tgttgtggga acttcagccc gtggaagtac gcgcacgtac
     1861 acgccctgcg aaattgacag caacactgga agcaacagag caggcggttt tttctcaagc
     1921 cggtgtcaat gtggctgatt ttcaggccta tatgcaggcg aataatctgg ctctgatcgt
     1981 cagtcataac gtcaccaccc gcgatcattc ggatgtgcaa cagccgctca acttacgtgt
     2041 gcctggtggt gcagaaacga tctttgcctc cgggaaggta tatgacgtgc aatacctgca
     2101 gatcttccag gccgatcaat tgcgtggaat tgggggcaca caatcaccaa ggccgggacg
     2161 aagggtactt gctcaaaatc ttcatgaccc ggctgttgcc ttcgttcaac cgcccaaccc
     2221 ggatggcccg gtggggagtg tggttctggg actggatggt tccatggccg cttttgtccc
     2281 tgcccggcgg gctttgacct ggcagttgac cgatccttcc ggggtggggg tggtgcgtga
     2341 gcgtgtgtgg gtgacgttac aaccgggtga gattcgtgtt tgtaaatcct gtcatggcgt
     2401 aaataccgcg gaccaggctg gcaacccgcc tcctgacaac gaaccgcagg cattgttgaa
     2461 tcttctggaa tattggaaag tggcggctaa cagccttttt gctgatgtgc ctgcttccca
     2521 ttgggcagcc agtttcattg agcgtttgta caatgcaggc attacaggtg gatgcggtac
     2581 ggcaccgttg ctttactgcc ccgaggcgac cgtgacccgc gcacaaatgg cgattttcat
     2641 tttgcgtgga atgcatggca gtgcctatg
//
LOCUS       DAOQLC010000296         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_23816_length_2608_cov_14.275754, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000296 DAOQLC010000000
VERSION     DAOQLC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23816_length_2608_cov_14.275754"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..813)
                     /locus_tag="PL000_21915"
     CDS             complement(145..813)
                     /locus_tag="PL000_21915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91597.1"
                     /translation="MMERILEPELMEDMEQVLAYARADFAASHQLRVTWFHERLGTSV
                     TPTSILDLCCGSGDMTFRFARAFSQSHILGVDGSQAMIEIARQDVQAEPDLAARIEFK
                     QAFLPDESLPRKGYDMVMSHSALHHFHDPRVLWDAIRMHSPSGSIVFVSDLRRVGSVA
                     EAKQIVNERAGNEHPLLQHDFAASLCAAFTPQEVQSQLDAAGLSQLNVEAIGDVYLLV
                     YGRV"
     gene            complement(810..2069)
                     /locus_tag="PL000_21920"
     CDS             complement(810..2069)
                     /locus_tag="PL000_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91598.1"
                     /translation="MSHKLHDVFPTLKVYVPAVLPGKRLKDSIVGLDTLDKETLLQVS
                     RVAGDAGQLLGHFGANVVTLVELPALFAPLQRSLSDLLESVQADYQALTVRGTEALSE
                     ETVRWQLPEGTPELHHGYNVCDHYFRFVRVKDMKAREWLGTLAFVSLAVVEDLPHTLL
                     NWDEEIALLASLTEMFSWILPEGMEAESSLQSGKPPISSETSLPLWQSEAKNVGRTIS
                     IAYYRLLIGHHIWQHINIFARECFEHSADEFAQGNDEEGTRWLWKATRLFRGTTASMW
                     YASIFPLQTYQAELRPTMVETDSIDAQQQHLTYNLLKQGIKQFKLTMEERAASNKPLH
                     SEQTYTVLKQFHEYYVQDMEQHILVASSKVGLDASLAQKVWQSKLPANTRTKNAMDLL
                     RDMAGIRRKDWQKVLSIAVAQPEMEGT"
     gene            complement(2066..2395)
                     /locus_tag="PL000_21925"
     CDS             complement(2066..2395)
                     /locus_tag="PL000_21925"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNC91599.1"
                     /translation="MMIVQATVIEVEKNNVPEDALLPLEKNGHEVAVCKVGQAYYAFH
                     NNCTHEEWKLSDSYMVDGHIVCSLHGAMFDPTNGVCKRGPACDPLRMYKVVEQDNKLF
                     IDLENEQ"
     gene            2394..>2608
                     /locus_tag="PL000_21930"
     CDS             2394..>2608
                     /locus_tag="PL000_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91600.1"
                     /translation="MNLAIKIFQKQLTRVYNMCVSLNSYSTPPKVGNSAHPKVLVIRT
                     TGWLYFTAGELYEHKNINDIQRPCPWG"
BASE COUNT          670 a          699 c          603 g          636 t
ORIGIN      
        1 gcccgcatca aatccctgcg cacagtggcg gaaaaattgc ggcgggcgtt gaagtaaacc
       61 agcccctgct tttgaagcat tcccgccgcc gaaggcttcg gcggcgggaa tatttatgta
      121 caattcgttt gcagaaagca aaatctacac cctgccatat accagcaggt aaacatctcc
      181 aatggcttcc acattcaatt gacttaatcc agccgcatca agttgactct gcacctcctg
      241 cggggtaaat gccgcacaca gcgatgcggc gaagtcatgc tgcaacaacg gatgttcatt
      301 gccagcccgt tcattgacaa tctgctttgc ctctgccaca ctgccaacgc ggcgcaggtc
      361 tgaaacaaaa acaatgcttc cagaagggga atgcatccga atcgcatccc acagcaccct
      421 tgggtcgtga aaatggtgca aggcactgtg agacataacc atgtcatacc ccttccgggg
      481 aagagattca tcgggtagaa aggcctgttt gaactcaata cgcgctgcca gatctggctc
      541 tgcctgcaca tcctgtcggg caatctcgat cattgcttgt gagccatcca cgccgaggat
      601 gtggctctgc gaaaatgcac gggcgaatcg aaaggtcata tcgccagagc cgcagcacag
      661 gtccaaaatg gaagtggggg ttacactcgt gccaaggcgt tcatgaaacc atgtcacccg
      721 caattggtga gatgcggcaa aatctgcacg cgcatacgcc agcacctgtt ccatgtcttc
      781 catgagttca ggctcgagaa tgcgttccat catgtacctt ccatctcagg ctgtgccacg
      841 gcgatgctta aaactttttg ccaatcttta cggcggatac ccgccatatc ccgcaggaga
      901 tccatggcgt ttttggtgcg ggtattggca ggcaatttgc tttgccacac cttttgcgcc
      961 agcgaagcat caagcccaac cttggaagac gcaaccaaaa tatgttgttc catatcctgc
     1021 acataatatt cgtgaaactg cttgagaacc gtataagtct gctctgaatg caaaggtttg
     1081 ttcgaagcag cgcgctcttc cattgtgagc ttgaattgtt taattccctg cttcaacaga
     1141 ttataggtca agtgttgctg ttgggcatca atgctgtctg tctccaccat ggtggggcgc
     1201 agttcagcct gataggtctg caatggaaaa atacttgcat accacatact tgccgtggtg
     1261 ccgcggaata ggcgggtagc cttccataac cagcgtgtac cctcttcatc gttgccttgc
     1321 gcaaattcat cggcgctatg ttcgaaacac tcacgcgcaa agatattgat atgctgccag
     1381 atgtggtgcc cgatgagcaa acgataatac gcgatggaga tggtgcggcc gacgtttttc
     1441 gcctcgctct gccataacgg aaggctggtc tctgacgaaa ttggcggttt gccactctgc
     1501 aagcttgact ctgcttccat tccctcaggc agaatccacg aaaacatttc ggttaatgaa
     1561 gccagcaggg caatttcctc atcccaattc aataaagtat gtggaaggtc ctccactaca
     1621 gccaacgata caaaagccag ggtacccagc cattcacgcg ccttcatatc cttcacacgc
     1681 acaaagcgaa aataatgatc gcagacattg tagccgtgat gcaattcagg cgttccctca
     1741 ggtaattgcc agcggacagt ttcctcgctc aatgcttcgg taccgcgaac ggtcaaagcc
     1801 tgataatcag cctgcacaga ctcaagcaag tcagaaagag atctctgcag gggtgcaaac
     1861 aaagcgggca attcgaccaa tgtcactaca ttggcaccaa aatgcccaag caattgaccg
     1921 gcatcgcctg ccactctgga cacctgtaaa agagtttctt tgtcgagggt atcaagccca
     1981 acaatcgaat cttttagacg tttgccaggc aacactgctg gcacatagac tttcaaggtg
     2041 gggaatacat catgtaattt atggctcatt gttcattctc caggtcaata aataatttgt
     2101 tgtcttgttc cactactttg tacatccgca atgggtcaca ggctgggcca cgtttgcaga
     2161 ccccgttcgt cgggtcaaac atggctccat gcaaagagca aacgatgtga ccatccacca
     2221 tgtagctgtc gctcagtttc cattcttcat gtgtgcagtt gttgtgaaag gcataataag
     2281 cctgacccac cttgcagaca gccacctcat gtccattctt ctccagtgga aggagggcat
     2341 cctctggaac gttgttcttt tcaacttcga tcactgtagc ttgaacgatc atcatgaacc
     2401 tcgcaataaa gatttttcag aagcaactca cgcgagtata taacatgtgt gtttcactca
     2461 acagttacag cactccccca aaggtgggga attccgctca tccaaaagtc ttggtcattc
     2521 gtaccaccgg ttggttatac ttcactgcag gagaacttta tgaacacaaa aatattaatg
     2581 acatccagcg cccttgtcct tggggcag
//
LOCUS       DAOQLC010000297         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_23967_length_2599_cov_2.897799, whole genome shotgun sequence.
ACCESSION   DAOQLC010000297 DAOQLC010000000
VERSION     DAOQLC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23967_length_2599_cov_2.897799"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PL000_21935"
     CDS             complement(<1..1097)
                     /locus_tag="PL000_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91601.1"
                     /translation="MTRFSSLSILRSTAMSLTVIGALGLGVCRGQSVLAQAAGKANLS
                     NAQLTALVRDPSVGSRALSVTVVGSGPLVTVLAEEKEIAQDKDLKIDAMFLARTLIDG
                     APGQIEKVKVLFSQTGKPARYVTISRGEITDFSAGRVSAATLLQSLALVAVHAENMPE
                     ITDGPEVERRLLAWKRIDKLRAQGTGVSPFEAIFKEIQGLAKANDAEKVSQRLSYLES
                     KLTGQEEQVKQAKDSLRGRGVPAVKTASSASAGAGAGTTAPGGQGAAPPQGAGGGYIP
                     PEAEHLKATFNAQSNEIISQVTRKNSSEGYKLRDLKSEIEKAFAAHQEARAYEMIHNF
                     DLAATKVLGSSAFGPGGQGGGMGPGGGMGQGG"
     gene            complement(1189..1782)
                     /locus_tag="PL000_21940"
     CDS             complement(1189..1782)
                     /locus_tag="PL000_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91602.1"
                     /translation="MTYSQNGKGHILSLKSVHLSALVAVFGSTTSLIAIPVAAQQQQQ
                     QQQQQQQQQQGQPDPRQVELVKRLGLSRNMTGPPAYALRQSEHFSTSAPTNFPIEIYR
                     SNVTATGFLNSTKGSPSASLSIATKDSLEVVSQFYQTVLRNGGWKFQTPSAEAQAKMG
                     QIYMLRAQKDKQNANIMLLNKQGEPGTNISINWYLSP"
     gene            complement(1807..>2599)
                     /locus_tag="PL000_21945"
     CDS             complement(1807..>2599)
                     /locus_tag="PL000_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91603.1"
                     /translation="PGKPAEKALAWTLTNPRQPFKMAIPHSFIHIKVEKPKAKFFFFI
                     PAPIENKEIDYGFGAPSMETATMPFGGVGCSSVTGNNIPIGVETAFRSVDEVIFDLPE
                     GNKAELENQLTARCNEMISQVGGTITKSEMHTALDSAICRAALVAGQRDFYVYSPDGK
                     NIHCSPLIQAVAEAPWLGVHSSKTPDGNEVTTIDAKGFPFFVFPPIVSPLPFFTPVPF
                     PVSSTSERYFKEVFWKPGTGYNHCLGEVRVKRSTEVYIIGVSVFP"
BASE COUNT          552 a          662 c          668 g          717 t
ORIGIN      
        1 ccgccctgtc ccatgccccc gccaggaccc atgccgccac cctgccctcc cggtccgaag
       61 gctgaggagc ctaagacctt ggtggcagcg aggtcaaagt tgtggatcat ttcgtaggct
      121 ctcgcctcct ggtgcgccgc aaaagccttt tctatctctg acttcaagtc tctgagcttg
      181 tagccctcgc tgctgttctt gcgggtaacc tggctgataa tctcgttgct ttgcgcgttg
      241 aaagttgcct tgagatgttc cgcttccggt ggaatatacc cgccgcctgc tccctgtggc
      301 ggcgcagcgc cctgccctcc gggggcggtt gtgcctgcac ccgcaccggc tgaagcgctt
      361 gaagctgttt tgacagcagg tactcctcga ccacgcaggg agtctttggc ttgcttgact
      421 tgctcttctt gtccggtaag ctttgattcg agataggaca atcgctgaga gactttctcg
      481 gcatcattgg cttttgccag tccctggatc tccttgaaga tagcctcaaa cggggaaacc
      541 ccagtacctt gagcgcgcaa tttgtcgatg cgcttccacg ccagcaggcg tctttccact
      601 tccggcccat cagtaatttc cggcatgttc tcggcgtgta cggcaaccag ggcgagcgac
      661 tgcagcaagg ttgccgctga cactcgtccg gcgctaaagt ctgtgatttc gccccgggaa
      721 attgtgacgt aacgtgcagg cttgcctgtc tgactgaaaa gcactttcac cttttctatc
      781 tgaccgggag cgccatcaat tagagtgcga gcgagaaaca tcgcatcgat tttcaaatct
      841 ttgtcctggg caatctcttt ttcttctgcc aaaaccgtga cgagcggacc gcttcctaca
      901 accgtaacgc tgagtgcgcg tgatccaacg gacggatctc gcaccagagc ggtcagttga
      961 gcgtttgaca aattggcttt tccagccgcc tgcgcaagca cgctctgtcc gcggcaaacg
     1021 cccagcccga gtgcgccgat tactgttagg ctcatcgcag ttgagcgcag gattgataag
     1081 gacgaaaatc tggtcatgaa gactctaccg ttgagtgtgg acaaaatccc aatgttggaa
     1141 ttttaccagc cctcggcggt ttgcgcgttc ggttgaagag gacgctgctc agggagagag
     1201 gtaccaatta atggagatat tcgttccggg ttcgccttgc ttatttagaa gcatgatgtt
     1261 cgcattctgc ttatctttct gcgctctcag catgtaaatt tgccccatct tcgcttgggc
     1321 ttctgccgaa ggcgtctgga atttccatcc gccgttgcgc aataccgttt ggtaaaactg
     1381 agaaacaacc tccaggctgt cttttgtcgc aatgctcaaa ctggctgaag gtgagccttt
     1441 tgtagagttc aagaagccgg ttgccgtgac gttgcttcta tatatttcga taggaaagtt
     1501 agtaggagca gaagtagaaa agtgttcact ttgtcttagc gcatatgcgg gcggtcctgt
     1561 catattgcga gacaatccta gtctctttac tagctcaacc tgcctgggat ccggttgacc
     1621 ttgttgttgt tgttgttgtt gttgttgttg ttgttgttgt tgttgagctg caacagggat
     1681 agctatgagg ctggtcgtcg aaccaaaaac agctaccagc gcgctgagat gtactgattt
     1741 tagggataat atgtgtccct ttccattctg gctgtatgtc atcgctgctc cgttttttgc
     1801 tcgagtctat gggaaaacac tcacaccgat aatgtatacc tctgttgagc gctttactct
     1861 tacttcacca aggcaatgat tatagccagt gccgggtttc cagaagacct ctttgaagta
     1921 cctctctgaa gtggaggaaa caggaaaggg tactggggtg aagaaaggga gaggcgaaac
     1981 aattggcgga aacacgaaga acggaaatcc ttttgcgtca atagtcgtta cttcattccc
     2041 gtcaggagtc ttgcttgaat gtacacctaa ccaaggcgct tcagcgaccg cctggataag
     2101 cggactgcag tgaatgtttt taccgtcagg actatagaca tagaaatctc gctgtcctgc
     2161 tactagcgca gctctgcata ttgctgaatc cagggcggta tgcatttctg attttgttat
     2221 tgtgcctccg acctggctaa tcatttcgtt gcaccgcgca gtaagttggt tttccagctc
     2281 ggctttgttt ccctctggta aatcaaaaat gacttcatca acagaacgga aggctgtttc
     2341 taccccaatg ggaatattgt tgcccgtcac agacgagcag ccgacccctc caaatggcat
     2401 tgttgcggtc tccatcgagg gagcgccaaa tccataatct atttctttat tttcaatcgg
     2461 cgcaggaatg aagaagaaaa attttgcttt cggtttttct acctttatgt gaatgaaaga
     2521 gtgcggaatt gccattttga atggctgacg cggatttgtt agcgtccatg caagagcctt
     2581 ttcagccggt ttgcctggt
//
LOCUS       DAOQLC010000298         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_24523_length_2566_cov_13.666268, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000298 DAOQLC010000000
VERSION     DAOQLC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24523_length_2566_cov_13.666268"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..346)
                     /locus_tag="PL000_21950"
     CDS             complement(203..346)
                     /locus_tag="PL000_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91604.1"
                     /translation="MLTAMGILVDESRIPLIRRAAAARIAGMCAFFYSEESEDTCRIG
                     KKD"
     gene            complement(348..1463)
                     /locus_tag="PL000_21955"
     CDS             complement(348..1463)
                     /locus_tag="PL000_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91605.1"
                     /translation="MSAANFQLAPTFLWVNQNIDNAVIIEPPVQMYRWSGSGPRRYFC
                     EWDKDLHWFLSVTTTIDAVSPTSSYLIEWIARHGVERARQIAKESADYGTLMHIVISA
                     FLRSREMDISLPAMEAAVAFHVKQEGILYDVSSWPEQLRRDMLSFAQFCADVRLEPVA
                     IECVLPWLKESIGGAIDCVAWVDIKEKGFWGEIYKSGPNKGQPKETFRVRRALVMIDW
                     KSCRKGFYVEHEIQLEIYKRIWHSWFLETLPIEFLCNWRPTEWSKEPSYHLKDQTGRH
                     SENEIAHIIGLAKERLSLVPPAVYQYPEKIVLQTIPESVIQIAAQDVAKSVFAEVLPA
                     AGGESNGKEKKKRKSHRSVSDGSKSKRKARRKRNAGE"
     gene            complement(1654..1842)
                     /locus_tag="PL000_21960"
     CDS             complement(1654..1842)
                     /locus_tag="PL000_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91606.1"
                     /translation="MKINASIEARVKSGNEGRGFYKVEIGKTRDVEVECTFEEILRQA
                     QEDAKRIAELDRLRRQML"
     gene            complement(1839..2243)
                     /locus_tag="PL000_21965"
     CDS             complement(1839..2243)
                     /locus_tag="PL000_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91607.1"
                     /translation="MKTFFTLVATGLLVVLVIQMAVEFRPEHMCVIRGLVVFLGGTFV
                     FVVSVSGAMAGYYEEREDGWHSISFRFFLFACPASRIILVFVFLPLVAMAFINGYVIG
                     GCLSGLAWCFVFYVIELYYRNAKRVSKMRSEK"
     gene            complement(2344..>2566)
                     /locus_tag="PL000_21970"
     CDS             complement(2344..>2566)
                     /locus_tag="PL000_21970"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HNC91608.1"
                     /translation="LGWAVAKGLVPMWAMVGAYLVGASLVGANLVGANLDGANLVEAN
                     LDGARIPAKYKEQFSEQGYNLEGVIWIKD"
BASE COUNT          657 a          746 c          517 g          646 t
ORIGIN      
        1 attctcgtaa tacattttta ctctcctcct ctgttttcaa tcgggcgtcg cacttgccat
       61 ctgcgatacc ccacctttct gcgtgcccac cgttctgctt cggcagcgta tgcgaaacct
      121 tctcgcacga gaatccaatc aagattatcc tcacgcaccc acacggtaaa accaacgggc
      181 gtgcgataac tcaatacatc gtttagtctt tctttccaat acggcatgtg tcctccgatt
      241 cttccgaata aaaaaatgcg cacatccctg cgatgcgtgc ggcagccgca cggcgaatca
      301 atggaatccg tgattcatct acaaggattc ccatcgccgt cagcatatca ctcaccagcg
      361 tttctttttc tgcgagcctt gcgcttgctt ttagatccat cagaaacgct tctgtgactc
      421 tttcttttct ttttttcttt gccattcgat tcaccacctg cggcaggtaa cacctccgca
      481 aaaactgatt tagctacatc ttgcgccgcg atctgtatca ctgattccgg aatcgtctgc
      541 aaaacaattt tctccggata ctgatacact gcaggcggaa caagactgag tcgttccttc
      601 gcaaggccga tgatatgcgc aatctcattt tcagaatgcc ggcccgtttg atccttcaga
      661 tgatagcttg gctccttgct ccactcagtc ggacgccagt tacacaaaaa ctcaataggc
      721 aaagtttcga ggaaccatga atgccagatc cgtttgtaaa tttccaattg gatctcatgc
      781 tcaacataga accccttgcg gcatgacttc caatcgatca tcacaagcgc acggcgcacg
      841 cggaaagttt ctttaggctg gcccttgttc gggcccgatt tgtatatttc gccccagaaa
      901 cctttctcct tgatgtccac ccatgcgacg caatcgatcg caccgccgat gctttccttc
      961 agccagggaa gcacgcattc aatcgcgaca ggctccagac gcacatccgc acaaaactgt
     1021 gcgaacgaaa gcatatccct gcgcaattgc tcaggccagg aggaaacatc ataaagtatg
     1081 ccctcctgtt tcacgtgaaa cgctacagcg gcctccattg ccggcagaga aatatccatc
     1141 tcgcgcgatc gcaaaaacgc agagataacg atatgcatca gcgtgccgta gtctgcagat
     1201 tcctttgcga tctgacgagc acgctcaaca ccatgccgag cgatccattc gattagatac
     1261 gatgaggtag gcgataccgc gtcgatcgta gtggtaaccg aaagaaacca gtgaagatcc
     1321 ttgtcccatt cgcaaaaata tctgcgcgga ccggaaccag accagcgata catctgaacc
     1381 ggtggctcaa tgatcaccgc attgtcgatg ttttgattca cccacaaaaa agtgggcgcg
     1441 agttgaaagt ttgcagcgct catgataggc tagcctcgct tttgaattcg ccgcaccagt
     1501 catcttttgc aaccacaggc cagatcgctt ttccattgtg ctccgcaggc ggaagtttat
     1561 tgcaagtata caatttcaca gaaggagact gcggtacatc atgcaccaaa caatagcgac
     1621 aaagctcgca tacttctgat attgatttct tcttcatagc atctgcctcc gcagacgatc
     1681 aagctccgct attcgctttg catcttcctg cgcctggcga agtatttctt caaacgtgca
     1741 ttccacttct acgtctcgcg tctttccgat ttccactttg taaaacccgc gtccttcgtt
     1801 tcctgacttc acgcgcgctt ctatgcttgc attgatcttc atttctcact ccgcattttt
     1861 gaaactcgtt tagcattccg atagtacagt tctatcacgt agaaaacgaa acaccatgca
     1921 aggccagaga ggcacccgcc gatcacgtat ccgttgataa aggccatcgc cactaacgga
     1981 agaaagacaa agaccagaat gatccttgat gcaggacatg cgaagagaaa gaaccggaat
     2041 gatatggaat gccaaccatc ctccctctcc tcatagtatc cggccatcgc tcccgaaacg
     2101 gaaacaacga acacaaatgt gccgccaaga aaaacgacca acccccgaat gacgcacata
     2161 tgctctggcc gaaactcaac ggccatctga atcacaagca ccaccagcaa accagtggcg
     2221 actaacgtaa aaaaagtttt catctatcgt gttcctccaa aaaattcgcc tcatcagcag
     2281 ttttgattag caacgccaga agatcatccg aagcggtgtg aaacatgatt gtaatctctt
     2341 ctctcagtct tttatccata taactccctc caaattgtat ccttgttctg agaactgctc
     2401 tttgtatttt gctggtattc ttgccccgtc gagatttgcc tcgacgagat ttgccccgtc
     2461 gagatttgcc ccgacgagat ttgccccgac gagacttgcc ccgacgagat atgccccgac
     2521 cattgcccac atcggcacaa gtcctttggc gactgcccaa cctaaa
//
LOCUS       DAOQLC010000299         2532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_25126_length_2532_cov_14.329834, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000299 DAOQLC010000000
VERSION     DAOQLC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25126_length_2532_cov_14.329834"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PL000_21975"
     CDS             <1..494
                     /locus_tag="PL000_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91609.1"
                     /translation="PSKWRLPRMSTAKRTKFQIRSDAAKQRWVRDDRKEAFWRKHIEA
                     WKASGMSKRAYSRAHNLSESSFNAWIREIALREREYVPTENVATLLADSKNPFVPIRL
                     VSDNEGKAAPLQPVPQVITPPHDSRVEIVVPGGAVMRVSDTCNVEFVAQLFSFLKSGD
                     SAC"
     gene            488..874
                     /gene="tnpB"
                     /locus_tag="PL000_21980"
     CDS             488..874
                     /gene="tnpB"
                     /locus_tag="PL000_21980"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HNC91610.1"
                     /translation="MLNLDPSTRVFLCSQLIDMRFSFDALCGLVTTYFGMNPLNGHVF
                     VFFSRRRDRMKVLFWDIDGFALYYKRLERGSYSWIMELGLEANGEMQASDFSLILAGI
                     NPYPSSQKRTGKRLKQSTEVQAVAQL"
     gene            892..2466
                     /locus_tag="PL000_21985"
     CDS             892..2466
                     /locus_tag="PL000_21985"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HNC91611.1"
                     /translation="MQNTQHHELSKEFLVTLDQEALINLVIQLDSKYQQLAENARIAL
                     AEKYGRKTEHFISPGQLLLFPSPSTNSGSLDSQDSAQKLGVKTNQQGHTRNQKPDLPH
                     VQLFPPPPNPALLPCPCCGVARVPTRRILQASRYQIVPARFYFEDLYSQVYSCKNCQC
                     AEELVIETPEIVENGTASAGLLAHIAVSRDCDHIPFHRQSAIYKRSGVQLSRSTLSDF
                     YSQTARILQPLHDFSRQVLLKSHVISTDDTPVKVIDRSKSRNMKLGRIWGYLGDETHP
                     VTLFDYTHSRGRDGPMTFLTGFNGRLQGDCFSGNLAICAAIDTVLVACNAHARRYFIK
                     SLPNNKLGAGNALSAFQQLYEIERTAKELGLSPENTQLMREQEAVPILKEFHRWLQQE
                     QLAAQPKSAYGKAIFYCLNNWTSLTEYVRDGRLSIDNNHCEREMKYIAMGRKAWLFFG
                     SDEGGKNHAIILSMIATCRRHGVEPWAYLTDVIQRLSENPNENLEDLLPYNWNPRYLT
                     GSPAEITQFQPTPKVA"
BASE COUNT          665 a          693 c          583 g          591 t
ORIGIN      
        1 ctccgagcaa atggaggctg cccagaatgt cgaccgcaaa gagaaccaaa tttcaaattc
       61 gcagcgatgc tgccaaacag cgttgggtcc gcgatgatcg caaagaagcc ttttggcgaa
      121 agcatattga ggcatggaag gccagcggca tgtcgaagcg tgcctacagc agggcacaca
      181 acttgtccga gtcttcattc aatgcctgga ttcgagagat agctctgcgg gaaagagagt
      241 atgtgccgac agaaaatgtt gccacgctct tagcggattc caaaaatcca tttgtgccaa
      301 tcaggcttgt gtctgacaac gagggcaaag cggcacctct ccagccggtg ccccaggtga
      361 ttacgccgcc gcacgattca cgagtggaaa ttgttgttcc tggtggtgcg gtaatgcgcg
      421 tcagtgatac ctgcaatgtt gaattcgtag cgcaactgtt ttcctttctc aaatccggag
      481 attctgcttg ctaaatctag atccatcaac tcgagttttt ctttgttcgc aactaatcga
      541 tatgcggttt tcctttgatg ctctctgcgg ccttgtcact acatatttcg gcatgaatcc
      601 cctcaatggt catgtcttcg tattcttcag tcgtcgccga gaccgcatga aggtcctctt
      661 ttgggacatt gacggtttcg cactgtacta caagcgcctg gagcggggca gctattcgtg
      721 gataatggaa ctcggtcttg aagcaaatgg tgaaatgcag gcctccgatt tctccttgat
      781 tctcgccgga ataaatccat atcccagttc gcaaaaacgc accggcaaaa gattgaaaca
      841 aagcaccgaa gtccaagctg tggctcaatt gtagttgata taattcctgc catgcaaaac
      901 acgcagcacc acgagctttc aaaagagttc ctcgtcaccc tcgatcaaga agcgctgatc
      961 aatctggtca ttcagcttga ttctaagtat cagcagctcg ctgaaaacgc tcgcattgcg
     1021 cttgccgaaa agtacggcag gaagaccgaa cacttcatta gtccagggca gctgttgctt
     1081 ttcccctctc cgtcaacaaa ctcaggatcc ttggattcgc aagactcggc ccagaaacta
     1141 ggcgttaaga ctaatcaaca gggccataca cgcaaccaga agccggatct gccacacgtt
     1201 caacttttcc cgccaccgcc aaacccagcg ctgcttcctt gcccgtgctg cggtgtagca
     1261 cgtgtgccaa cgcgacgaat tcttcaagct agtcgctacc aaatcgttcc agctaggttc
     1321 tactttgagg atctgtactc ccaagtttac tcatgcaaga actgtcaatg cgcagaggaa
     1381 cttgtgattg agacgccgga aattgtagaa aacggcacgg cttccgccgg attgcttgct
     1441 cacatagcag tctcccgtga ctgtgaccat attccgtttc accgtcagtc agccatttac
     1501 aagcgcagtg gagtccagct cagtcgctcc actctcagtg atttctactc tcagaccgcc
     1561 agaatcctcc agccactgca cgacttcagt cgccaagttt tgctcaaatc tcacgtgatc
     1621 tccacggatg atacgcccgt aaaggtaatt gaccgctcta aatcgagaaa catgaagctc
     1681 ggtcgcatct ggggttactt gggagatgaa acccatcctg taacactctt cgattacact
     1741 cacagcagag gccgtgacgg tccaatgact tttctcaccg gcttcaatgg tcgacttcag
     1801 ggtgattgct tctccggcaa tctggctatt tgtgccgcta tcgatactgt tcttgtggcg
     1861 tgtaatgcgc atgctaggcg atatttcatc aagtcgctgc caaacaacaa actcggcgcc
     1921 ggtaacgcac tttctgcctt tcagcaactc tacgaaatcg aaagaacggc gaaggaactc
     1981 ggtctgtcac cagagaacac tcaactgatg agagagcaag aagctgtgcc gatacttaaa
     2041 gagttccacc gctggttgca acaggaacaa cttgctgcgc agcccaaaag cgcatacggc
     2101 aaggcaattt tctactgcct gaacaactgg acatctctca ccgaatacgt cagagacggc
     2161 agactcagca ttgataacaa ccactgcgaa agagaaatga aatatatcgc catgggacga
     2221 aaggcatggt tgttcttcgg cagcgacgag ggcggaaaga atcacgccat aatcctctcg
     2281 atgatcgcaa catgcagacg ccacggcgtc gaaccctggg cctacttaac cgatgtaatc
     2341 cagcgattga gcgaaaatcc caacgaaaac ctcgaagacc tgcttccata caactggaat
     2401 cccagatatc tcactggctc gccagccgaa atcacccagt ttcagcctac cccaaaagtc
     2461 gcttagccat cctgtaattt ggtaaacacc caatccatgg gactttacca aattgcacga
     2521 tgaagcataa ga
//
LOCUS       DAOQLC010000300         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_25162_length_2530_cov_28.796364, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000300 DAOQLC010000000
VERSION     DAOQLC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25162_length_2530_cov_28.796364"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..689)
                     /locus_tag="PL000_21990"
     CDS             complement(18..689)
                     /locus_tag="PL000_21990"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNC91612.1"
                     /translation="MTSKNDLALERAVEGKVIYLLALIVLVQFAYPITANGTLALTVY
                     VILYASMLAVGILLGRDSRFHTLFLMVTGISYLAASLIYASMPTAIWAVIITYITLIP
                     YLGMLVWILGRFLVVVQTITRDVLYAAVALYLLLGAIFVPIYGLLDFIIPNSFRDGTF
                     PDAPVQWQQFVYFSYTTLTSSGYGDILPVSWWARSFANLEMITGVLFITIVMSRLVSL
                     YNSDK"
     gene            complement(727..1785)
                     /locus_tag="PL000_21995"
     CDS             complement(727..1785)
                     /locus_tag="PL000_21995"
                     /inference="COORDINATES: protein motif:HMM:NF003367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HNC91613.1"
                     /translation="MSETLIGLVSQYSPSGQERGAVEWLVARMKSLGYDNAFIDDAGN
                     AVGVMGKGPKQVILLGHIDTVPGEIKVVRDGISLYGRGSVDAKGPLASFTDAVAKVGA
                     KDGWQFVVIGAVEEERNSEGARFVVNQYKPDFAIIGEPNQWDRMSLGYKGSAWANVTV
                     RRGQVHTASGEETAAEAAVELWLKIKTFVDSFNADKSKVFDKLLLTLRGMESDSNDFE
                     QWAKLKIGVRLPVEISPEDWYRKLEEIISDSHLHGDCHLERVGFPVAAWKCEKNSPLV
                     RSFLSGIRSQGGEPRFVYKTGTADLNIVAPVWQCPALVYGPGDSALDHTPNEHIDLTD
                     YQKAVDVLAAAMAKLVEA"
     gene            complement(1866..>2530)
                     /locus_tag="PL000_22000"
     CDS             complement(1866..>2530)
                     /locus_tag="PL000_22000"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC91614.1"
                     /translation="DKAVNEETAAVILEVVQGEGGVYPASLEYVQAARRICDERGALL
                     IIDEIQSGFGRTGKMFAIQHFGVTPDLLTCAKSLAGGVPMGAVLIGPNVKNLTPGVHG
                     STFGGNPLSCAAANAALDVMKDEDLPGQAAAKGAYLMEKLKALQSPNIREVRGLGLMI
                     GIEMKQKVAGYIKALQERKIIALNAGMTVIRLLPPLVITYEQIDHLVDVLADVLKQGL
                     EE"
BASE COUNT          655 a          725 c          561 g          589 t
ORIGIN      
        1 ccccgcctgg catcatgtta tttatcggaa ttatataaac tcaccagccg cgacatcaca
       61 atggtaataa aaagcacacc tgtgatcatt tctaaattgg caaaagaacg cgcccaccac
      121 gagacgggca gaatatcgcc atagcccgat gacgtaagcg tggtgtatga aaaataaacg
      181 aactgctgcc attgcacggg cgcatctgga aaggttccat cgcggaacga gtttgggatg
      241 atgaaatcca acaaaccgta gatgggcaca aagatcgcgc cgagtaaaag atacaatgcc
      301 actgcggcat acaacacatc gcgggtgatg gtttggacaa cgacaaggaa gcgtcccaag
      361 atccaaacca gcataccaag gtagggaatc agtgtgatgt aagtgatgat aaccgcccaa
      421 atggcggttg gcatggaagc atagatcagg ctggcggcga ggtagctgat tcccgtcacc
      481 atcaaaaaga gggtatgaaa acgggaatcg cgcccgagta aaattcccac agccagcatg
      541 gatgcataca aaatcacata caccgtgagt gcaagcgtgc cattggctgt gatgggatat
      601 gcaaactgca ccagcacgat caacgccaac aggtagatca ctttgccttc gacggcacgt
      661 tcaagagcga ggtcattttt agaggtcatg gtgtgaaaaa gtcaaacttg tatttttgat
      721 gggtttctat gcttcaacca attttgccat ggcggctgcc agcacgtcca ctgctttttg
      781 gtagtcggtc agatcaatat gttcattcgg cgtatgatcc agcgccgaat caccggggcc
      841 atacaccaac gcagggcatt gccagaccgg ggcgacgatg ttcaggtcgg cggttcccgt
      901 cttatagacg aaacgcggct ctccgccctg tgacctgatt ccgcttaaga agcttcgtac
      961 cagtggtgag ttcttctcgc acttccacgc agcgacaggg aatcctacac gttccaagtg
     1021 acagtcacca tggaggtgac tgtcacttat gatctcttcc aatttacgat accagtcttc
     1081 aggtgagatc tccaccggta aacgcacacc gatcttcaac tttgcccatt gttcgaaatc
     1141 attcgagtca gattccattc cgcgcaaagt tagcaacaat ttgtcaaaca cctttgattt
     1201 atccgcattg aacgaatcta caaatgtttt gatcttcaac cataattcaa cggcggcttc
     1261 agcggccgtt tcttccccgc tcgcagtatg gacctgtcca cgccgcacag tgacatttgc
     1321 ccatgcactg cctttgtaac ccagtgacat ccgatcccat tgatttggtt caccgataat
     1381 ggcgaaatcc ggcttatatt ggtttaccac aaaacgtgca ccctcagaat tgcgctcttc
     1441 ttcgaccgcg ccaatgacca cgaactgcca tccatctttt gcaccaacct ttgccactgc
     1501 atccgtaaaa cttgccagcg gacctttcgc atccaccgag cctctgccgt aaagcgagat
     1561 accatctcgc actactttaa tttcacccgg caccgtatcg atgtgcccca gcagaattac
     1621 ctgcttcggc cccttcccca tcacaccgac agcattaccc gcatcatcaa taaacgcatt
     1681 gtcatacccc agtgatttca tacgggccac cagccattcc actgcgccgc gctcctgccc
     1741 cgacgggctg tactgagaga caagaccgat caaagtttcg ctcataattt cctgaccgca
     1801 aaccgcagac gttagaccgt gtacacgcca aatggcggtc agcagtccat ggtcggcgac
     1861 agctattatt cttccaagcc ctgcttcaaa acatcagcaa gcacatcaac caggtgatca
     1921 atttgttcat aggtaatcac caatggaggc agaaggcgaa ttacagtcat ccccgcgttc
     1981 aatgcgatga tttttctttc ctgcaatgct ttgatataac cagccacctt ttgcttcatt
     2041 tcaatgccaa tcatcaaacc aagtccgcgc acttcgcgga tgttcggcga ttggagcgct
     2101 ttcaacttct ccatcaggta tgcacccttg gccgcagctt gaccgggcag gtcttcatcc
     2161 ttcatcacat ccaacgccgc atttgccgcc gcacatgaaa gcggattgcc gccaaaagtg
     2221 gaaccatgca caccaggcgt taggtttttg acattgggac cgatcaacac cgcgcccatc
     2281 ggcacgccgc cagccaatga cttggcacag gtgagcaggt ctggggtcac gccaaaatgc
     2341 tggatcgcaa acattttccc tgtgcggcca aagcctgatt gaatttcatc aatgatcagc
     2401 aatgctccgc gttcatcgca gattctccgg gccgcttgta catactccaa actcgctgga
     2461 tatacaccgc cttcgccttg caccacttcc agaatcacag cagcagtttc ttcgttgaca
     2521 gctttatcga
//
LOCUS       DAOQLC010000301         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_27046_length_2432_cov_23.455196, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000301 DAOQLC010000000
VERSION     DAOQLC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27046_length_2432_cov_23.455196"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PL000_22005"
     CDS             complement(<1..706)
                     /locus_tag="PL000_22005"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91615.1"
                     /translation="MLERLDLTQALEREEYNKQLKALQAEVHELAFQVYAQKRPVVIV
                     YEGMDASGKGGTIKRLTERIDPRGYVVWPIAAPSGEDKERHYLYRFWRRLPEQGQIAI
                     FDRSWYGRVLVERLEGYCTEADWRRAYGEINQFERQLTDYGTIVFKFWMHISKQEQLR
                     RFRERTRTNYKAWKITDEDWRNRGKWEDYIIAAEEMLLKTSTPDSPWTLVEANDKYFA
                     RIKSLRTVAEKLRRALK"
     gene            complement(699..1478)
                     /locus_tag="PL000_22010"
     CDS             complement(699..1478)
                     /locus_tag="PL000_22010"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91616.1"
                     /translation="MLEYVNLDLAITQEEYKKQLRPLQQRLYELEHAVFDAKIPVIIV
                     FEGWAATGKGRLISLLAERLDPRGFRVVPITPPRTAETRYPWMWRFWQKIPARGQVVA
                     FDTSWYRRVLIDRLNKTINKGDLRAAYQDIAEFEEMLSADGTVILKFWLHLSEADQKK
                     RIKKLRKDKLTAWQVSEEDLLQNKRYEKYTAFVEDMIARTDSPHAPWTIVEATDRYYM
                     RIKVFETIIASLEARLGLPSSTGAKSKRPRKTKEMKGAVNA"
     gene            complement(1513..2289)
                     /locus_tag="PL000_22015"
     CDS             complement(1513..2289)
                     /locus_tag="PL000_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91617.1"
                     /translation="MNKDTLRQLANVLSVVIALTVNILASTLPLNGQNTGEISDRFQV
                     YFVPAGYVFAIWGIIYIGWIAFSIYQFRADQKESPRLRRLGYVFAVSGIFNAAWLFCW
                     HYNLFGLSVLVMLTLLGLLIAGYLKLNVGRTPVSAAEKWSVDIPFSVYLGWISVATVA
                     NVTDYLYFINWAGFGLAPQAWAVVMLVVACLLGLLMTVTRRDSGYVFVLVWSFAGIAQ
                     KQSAAALVANSAWVATLFMLGLAIYSMVERNRAGNGREVK"
BASE COUNT          502 a          636 c          693 g          601 t
ORIGIN      
        1 acttcaacgc ccgccgcaat ttttccgcca ctgtgcgcag ggatttgatg cgggcaaagt
       61 atttgtcgtt ggcttccacc aatgtccagg gggagtcggg tgtgctggtc ttcaagagca
      121 tctcttctgc cgcgatgatg taatcttccc atttgccgcg gttgcgccaa tcttcatctg
      181 tgatcttcca ggctttgtaa ttcgtgcgtg tgcgttcgcg gaagcggcgc aattgttcct
      241 gcttgctgat gtgcatccag aatttgaaga cgatggtgcc gtaatcggta agttgacgct
      301 cgaattggtt gatctcgcca taggcgcggc gccaatcggc ttctgtgcaa tagccttcaa
      361 ggcgttccac cagtacacgc ccataccagg aacggtcgaa gatggcgatc tggccttgtt
      421 cgggcaatcg ccgccagaaa cgatacaggt aatgccgttc tttgtcttcg ccagaggggg
      481 cggcgatggg ccagaccacg tatccgcgtg gatcgatgcg ctcggtcagg cgtttgatcg
      541 tgccgccttt gcctgaagca tccatgcctt catagacgat caccacagga cgtttctggg
      601 catagacctg aaacgccagc tcatgtactt ctgcctgcaa tgccttgagt tgtttgttgt
      661 attcctcgcg ttcaagggct tgggtaagat cgagtcgctc aagcattgac ggctcctttc
      721 atttcctttg ttttgcgcgg acgtttggac tttgcccctg ttgaggaggg cagtcccaga
      781 cgggcttcga gtgaggcgat gatggtctcg aagactttga tgcgcatgta atatcggtcg
      841 gtggcttcga caattgtcca gggggcgtga ggcgaatcgg tccgcgcgat catatcttcg
      901 acaaaggctg tatatttttc gtagcgtttg ttttgcagca agtcttcttc gctgacctgc
      961 caggcggtca atttgtcttt gcgtaatttc ttgatgcgtt tcttctggtc tgcttcgctg
     1021 aggtgcagcc agaacttcaa tatgaccgtg ccgtcggcgc ttaacatttc ttcaaattcg
     1081 gcgatgtctt gataggcggc gcgcaggtca cctttgttga tggttttatt caaacggtcg
     1141 atcaagacgc ggcgatacca ggatgtgtca aatgcaacca cttgtccgcg ggcggggatc
     1201 ttttgccaga agcgccacat ccacgggtag cgcgtttcgg cggtgcgcgg cggtgtgatg
     1261 gggacaacgc gaaagccgcg cgggtcgagc cgttctgcca gcaggctgat caaccgtccc
     1321 ttgcccgtgg cggcccaacc ctcgaacaca atgatgactg ggatctttgc gtcgaagacg
     1381 gcatgctcca gttcataaag gcgctgttgt aacggcctta actgtttttt gtattcttcc
     1441 tgcgtaatgg ccagatcaag gttgacgtat tcgagcatgg cgggctccag tttgttgggt
     1501 ttgacttggt tgttatttta cctctctccc gttccctgcc ctgtttcgtt ccaccatgct
     1561 gtagatcgcc aagccaagca tgaacagggt ggcgacccag gcgctgtttg ccaccagtgc
     1621 cgcagcggac tgtttttgag cgatcccagc gaaggaccaa accaacacga agacgtagcc
     1681 actgtccctg cgggtgaccg tcatgagcag gccaagcaga cacgcaacaa cgagcatgac
     1741 aaccgcccaa gcctgcggcg cgagtccaaa tccagcccag ttgatgaagt aaaggtagtc
     1801 ggtgacgttg gccactgtgg cgacggagat ccagccgagg tagacgctga agggaatgtc
     1861 cacgctccat ttttcagcgg cgctgacggg tgtgcgcccg acgttgagtt tgagataccc
     1921 cgcgatgagc agaccgagca gggtgagcat gaccagcacg ctcaagccga acaggttgta
     1981 atgccagcag aaaagccagg cggcgttgaa gatgccgctg acagcaaata catacccgag
     2041 cctgcgcagg cgcggacttt ccttttggtc ggctcgaaat tgatagatgc tgaatgcgat
     2101 ccagccgatg tagatgatgc cccaaatggc gaagacatac cctgcaggga caaagtagac
     2161 ttgaaagcgg tctgagatct cgcctgtgtt ctgcccgttg agcggcaggg tgctggcgag
     2221 gatgttgacg gtcagtgcga tgacaacgga tagtacattg gcaagttgac gaagagtgtc
     2281 tttgttcacg atggtctcca tttcgaaatg tgtgtcacag ggataggttc atcatacgac
     2341 ttcttactgt caattgctat catcaagagt gggtataagc tcaaaaatta ccttgacccg
     2401 cgcaaaccga cccccacccg tcctccccca aa
//
LOCUS       DAOQLC010000302         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_27660_length_2401_cov_19.629582, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000302 DAOQLC010000000
VERSION     DAOQLC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27660_length_2401_cov_19.629582"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..375
                     /locus_tag="PL000_22020"
     CDS             172..375
                     /locus_tag="PL000_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91618.1"
                     /translation="MVQEVRDARTQRNCVDKFFVEVLHDAFTEITGTISQFPAVDRKE
                     LYDAAQGLLNVALRHDTYGVFEK"
     gene            372..1097
                     /locus_tag="PL000_22025"
     CDS             372..1097
                     /locus_tag="PL000_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91619.1"
                     /translation="MKIAMNMLYLCWLVNTIANLPDTFWTGVLERRREEDEAFRANFR
                     QREEQMERQREQWKKRSIPFVFAPIVPGPTAPPPCNVPVHTSIERTFPPSTYLPPDHP
                     VRQNAVRDAKAWVANKVFDDFIWRDHRQVIVIPPAPPEAKVKQLKRAYFADDSDMYPG
                     YSPPEFWLPEYIREAELSSSDDDSLIQLLRREGAHDEAAQLIEQHRSHTVQTVVKLYM
                     EQRPDKYPQAAPGKVWIKQRKVA"
     gene            1094..1324
                     /locus_tag="PL000_22030"
     CDS             1094..1324
                     /locus_tag="PL000_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91620.1"
                     /translation="MIKQIEHLAVTPANANTVNVALFAEREAWAAWARNSSETLRREW
                     ATIAVVHQESLDGVTLARMVETALRLERMANE"
     gene            1321..1818
                     /gene="radC"
                     /locus_tag="PL000_22035"
     CDS             1321..1818
                     /gene="radC"
                     /locus_tag="PL000_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNC91621.1"
                     /translation="MNIVKTEDTPEYAVALVRDISEDDVVTQALEILERRLRKPGEAF
                     QSPATVKNYLSLYFAGLAHELFIMLLLDSQNRLISVSNMFRGTLTQTSVYPREVVKEA
                     LHFNAAAVILAHNHPSGFAEPSRADELLTEELKKALALVEVRVLDHFVVAGKDIVSFA
                     ERGLI"
     gene            1830..>2401
                     /locus_tag="PL000_22040"
     CDS             1830..>2401
                     /locus_tag="PL000_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91622.1"
                     /translation="MYTTLNDIRAFNPCKEGWKTLLKSLGKTKADDEPLSLLQVLSSN
                     GLSDTLWCFGVVDDFEKEKRLLAVAFAREVQHLIKDETLLSALDVAERYAYGLLTEQE
                     LARTQDAVYAVTVKAWGTEVTSEPANSIGFLVEACVEDHVYPLDAASAAQDAVWFHAL
                     STDHKNSVDALDRVQEKQQGLLRSMLERLT"
BASE COUNT          617 a          569 c          646 g          569 t
ORIGIN      
        1 attacaaacg agtttttgac aatcggatgt caacgtcact cacatgctga atgggaagct
       61 tttagtaacg atcaaattgc caaaatggaa agacgggcac ttaggttctg gcaagaatgg
      121 aagcgcccgc ttctcagcat gtgctcaatc cactccaaaa aggaggaata aatggtgcaa
      181 gaagtacgtg atgctcgcac gcagcgtaac tgcgtcgaca agttcttcgt ggaagttctc
      241 catgatgctt tcacggagat cactggaact atctctcagt tcccagctgt ggatcgtaaa
      301 gaactttacg acgcagctca gggcctttta aacgtggcac ttcgccacga tacctatgga
      361 gtattcgaga aatgaaaatc gcaatgaaca tgctctatct ttgctggttg gttaatacca
      421 tcgccaatct ccctgacact ttttggacgg gagtcttgga gcgtcgtcgg gaggaagacg
      481 aagccttcag ggctaatttc cgtcagcgcg aagagcagat ggaacggcag cgggagcagt
      541 ggaagaagcg gtccatccca tttgttttcg cacctatcgt tcctggacct accgcgccac
      601 ctccatgtaa cgtcccagtt catacctcca tcgagcgtac ctttccaccg tccacctatc
      661 ttccgcctga ccaccctgtg cgccaaaatg ctgtgcgcga tgccaaggcg tgggtggcta
      721 acaaagtttt cgacgatttt atttggcggg atcatcgcca agtgattgtc attccgccag
      781 caccacctga agccaaggtc aagcaactta agagggctta ctttgccgac gattcggata
      841 tgtatcctgg ctattcccct cctgaattct ggctccctga atatatccgg gaagccgagt
      901 tgtccagttc ggacgatgat tcactcattc agttgctgcg ccgggagggt gcgcatgatg
      961 aagctgcgca actcatcgag cagcatcggt cgcatactgt gcagaccgta gtcaagctct
     1021 acatggagca gcgtccggac aaatacccgc aagccgcacc gggcaaggtg tggattaagc
     1081 agaggaaagt ggcatgatca agcaaattga acaccttgca gtaactcctg ccaacgccaa
     1141 tacagtgaat gtggcattgt ttgcagaacg tgaggcatgg gctgcgtggg cacgcaatag
     1201 cagtgaaact ttgcggcggg agtgggccac aatagcagtg gtccatcaag agagcctgga
     1261 tggtgtaact ctggcaagaa tggttgaaac tgccctacgg ttagaaagga tggcaaacga
     1321 atgaacatag tgaaaaccga ggacacgccc gagtatgctg ttgcccttgt cagggacatc
     1381 tcggaggacg atgtcgtgac ccaggctctc gaaattctgg agcggcgctt acgcaaacca
     1441 ggggaagcat tccagtctcc agccacagtg aagaactact tgagccttta cttcgccggc
     1501 ctagcacatg agttatttat catgctactt ttggatagcc aaaacaggtt gatcagtgtt
     1561 tcgaatatgt tcagaggcac gttgacacaa accagcgtct atccgagaga agtggttaag
     1621 gaggcgttgc acttcaacgc agcggctgtg atcctggctc ataatcaccc ttctggcttt
     1681 gctgagccga gtcgggctga tgagctgttg accgaggaat tgaaaaaagc gttggcactt
     1741 gtcgaggtac gcgtactgga tcattttgtt gtcgccggta aggacatagt gtcgttcgca
     1801 gaacgcggct tgatttgaga ggaaccgtaa tgtacacaac cctgaatgat ataagggcgt
     1861 ttaacccttg taaggaaggg tggaaaaccc tcctaaagtc cctgggtaag accaaggcag
     1921 atgatgagcc cctgtctttg ctccaagtcc tgtccagtaa tggattgagt gacacactgt
     1981 ggtgttttgg tgtagtggac gatttcgaga aagaaaagcg gctattggct gtggcctttg
     2041 ctcgtgaagt gcagcaccta atcaaggatg aaacactgtt aagtgccttg gacgtggctg
     2101 agagatacgc ctatgggctg cttactgagc aggagctagc taggacacaa gatgcggtat
     2161 atgctgtgac agttaaggcg tggggcacag aagtcacgtc agaacctgca aacagtattg
     2221 gttttttagt agaagcctgc gtagaggacc atgtctaccc tctagatgca gcaagtgctg
     2281 cacaggatgc tgtgtggttc catgcactct ctacagacca caaaaactct gtagatgcac
     2341 tggatcgtgt acaggaaaaa caacagggtt tgttgcgctc tatgcttgag cgattaactt
     2401 a
//
LOCUS       DAOQLC010000303         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_27853_length_2392_cov_23.272144, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000303 DAOQLC010000000
VERSION     DAOQLC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27853_length_2392_cov_23.272144"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PL000_22045"
     CDS             complement(<1..504)
                     /locus_tag="PL000_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91623.1"
                     /translation="MKHTKFLLILLTALLISCAPTPAPVHFPTFLPTATANAASVPSS
                     VSIATLPAAATVETFYKFINAAQSPNDFNAAWDMLTNEEQCNTPEKCELSYFQEKWME
                     SKVIYKIYECGPDYAIVEERLYPRTADPASASSEARLIRFQLAQADEQIFITNRKTVM
                     ALEAGCVL"
     gene            complement(501..932)
                     /locus_tag="PL000_22050"
     CDS             complement(501..932)
                     /locus_tag="PL000_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC91624.1"
                     /translation="MSLFYFSITLAICSSALYHFVAKSTPSGVNFTVSLLVTYAVAFV
                     VTLFTFFFFPTKNGIAAELKQLNWASIGLAIAIVGIEFGFLLTYRAGWHLGIAAVLVN
                     VVASLILVPVALLVFKDEISWVNILGILICLVGLVMLNWKR"
     gene            complement(1068..1682)
                     /locus_tag="PL000_22055"
     CDS             complement(1068..1682)
                     /locus_tag="PL000_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91625.1"
                     /translation="MNKKQLTILFTLILFILSACGGAAEPTITAADVEGTAVANAWIA
                     LTQTQAALPTQTPSPIPSETPTIQPTMTAAALVLPTLAPPTLAVVTTPTNPADDPCNQ
                     PPPAEPKGLTVKVKFVNKSGGQAALSFGMNFPNKIGECGTYGYTLGPFDEPTVTVLAG
                     CYWGYAWITGDKPSVAKTGDAILCVQDPNEVRAIWITAENIGFH"
     gene            complement(1775..>2392)
                     /locus_tag="PL000_22060"
     CDS             complement(1775..>2392)
                     /locus_tag="PL000_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91626.1"
                     /translation="FAPNFPNIEALAKLVSSDSLAEPSQLLIRLHPSHFQDKPKIFAD
                     ERAQVFELEKKYPHVHVVQPVALGGSLGYYGGEDMDEKSSMMAYSDVVVTVYSTMLVE
                     TAVHDTPMIAATIDTPGGWNKPNKFSLSLKEIGDWPTHQRFRWAKAGRVAENEEQLRD
                     ALNLYLTDSTVDAAQRRKFVEDEITFTDATSGKRTAEFILKVLGK"
BASE COUNT          545 a          566 c          700 g          581 t
ORIGIN      
        1 cagaacgcag cccgcctcca gcgccatgac cgtttttcta ttcgtgatga agatttgttc
       61 atcggcctgt gccaattgaa agcggatcaa ccttgcctca gaacttgccg aggcgggatc
      121 ggcagttcgg ggataaagac gttcctccac gatggcataa tcggggccgc attcgtagat
      181 tttgtagatg accttcgatt ccatccattt ttcctgaaaa taactcagtt cgcatttttc
      241 aggtgtattg cattgctcct cgttggtgag catatcccac gcagcgttga agtcattggg
      301 agattgggcg gcattgatga atttgtaaaa ggtttcaacc gttgcagcgg cagggagcgt
      361 ggcaatggaa acactggacg gtacagaagc ggcgttggca gtggcggtgg gtaggaatgt
      421 gggaaagtgt accggggcag gtgttggagc gcatgagatg agaagtgctg tgagaaggat
      481 aagaaggaac tttgtatgtt tcatctcttc cagtttagca taaccagccc gaccaaacaa
      541 atcagaatgc cgagaatgtt cacccacgag atttcatcct tgaaaacaag gagcgccaca
      601 gggacaagga tgagcgatgc caccacattg accaatacgg cggcaatgcc caaatgccag
      661 cctgcacggt aggtgagcag gaatccgaac tcaatgccga cgatggcgat tgccagccca
      721 atgctggccc agttaagctg tttcaactcg gcggcgatcc cgtttttggt tgggaagaaa
      781 aagaaagtga acagcgtaac cacgaaagcg acggcatagg tcaccaaaag tgaaacggtg
      841 aaattcacac ccgaaggcgt gcttttggca acaaagtgat acagtgcgct ggaacaaatg
      901 gccagagtga tcgagaaata aaagagggac atggtttttg gtttcataat gagtaaaggg
      961 atgatacgca tcgtcccgaa ggttcttgct ggccttcggg acgtttctat cgggaagtgc
     1021 tggttttggt cagcccttcc cgttgagaat ggtcgaggtg ttttgggcta atggaagccg
     1081 atgttttcag ccgtgatcca aatggcgcgg acttcgttgg ggtcttgaac gcacagaatg
     1141 gcatcgcctg tcttggctac cgagggcttg tcgccagtga tccaggcata gccccaataa
     1201 caacctgcca atacggtgac ggttggttca tcgaatggcc caagggtgta gccgtaggtg
     1261 ccgcattcgc cgatcttgtt cggaaagttc atgccaaatg acagcgcagc ttgtccgcca
     1321 gatttattga cgaacttgac cttgaccgtg agccctttgg gttcggctgg cggcggctgg
     1381 ttgcagggat catccgctgg gttggtgggg gtggtgacca ctgccagagt ggggggagcc
     1441 agcgtgggca gaaccaatgc ggctgccgtc atggtgggtt ggatcgtggg tgtttcactt
     1501 ggaattgggg agggggtctg ggtggggagt gcagcttgtg tttgggtaag cgcgatccat
     1561 gcattggcta cggccgtgcc ctccacgtcc gctgcggtga tggtgggctc agcggcccct
     1621 ccgcaggcag ataggatgaa gaggatgagg gtgaaaagga tggtcagttg tttcttgttc
     1681 atttgtttct ccagccccca tttttaccac aaagagtttt aaaccgcgaa gcacgccaac
     1741 ggcggcaaag attttttctt tgccgccgtt atttttattt gcccaatact ttcaggatga
     1801 attctgcggt ccgctttcca gaagttgcgt ctgtaaatgt gatctcatct tcgacgaatt
     1861 tccgcctctg ggccgcgtcg acagttgaat ctgtcaaata aagatttaac gcgtcccgaa
     1921 gttgctcctc gttttctgcc acgcgccctg ccttcgccca gcggaaacgt tgatgcgtag
     1981 gccagtcgcc gatctctttc aatgagagcg agaacttgtt cggtttgttc caaccgcctg
     2041 gggtgtcgat ggtggcagcg atcatcggcg tatcgtggac ggcggtctcg accagcatgg
     2101 tggagtagac cgtcaccacc acatccgaat acgccatcat ggatgatttt tcgtccatgt
     2161 cttcgccgcc gtaatagccg agcgacccac ccaacgccac aggctggacg acatgcacat
     2221 gcgggtattt cttttccaat tcaaagacct gtgcgcgttc atcggcgaag atcttcggct
     2281 tgtcttgaaa gtgacttgga tgcaggcgga tcaacaattg cgaaggctct gcgagtgaat
     2341 cagaggaaac caatttcgcc aacgcttcga tatttgggaa gttcggcgca aa
//
LOCUS       DAOQLC010000304         2381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_28080_length_2381_cov_23.327171, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000304 DAOQLC010000000
VERSION     DAOQLC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28080_length_2381_cov_23.327171"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1067
                     /locus_tag="PL000_22065"
     CDS             40..>1067
                     /locus_tag="PL000_22065"
                     /inference="COORDINATES: protein motif:HMM:NF025114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNC91627.1"
                     /translation="MESIETIIIGGGQAGLATSYHLKQLGREHIIFEAADIPAHVWRD
                     DRWDSFTFVTPNWAIQLPGAGYEGRDPDGFLKKDEILAYFEGYVEKYQFPIQLNTTVT
                     EVAPVEGGYQVIANGKTYHAKNIVMATGSFQKPKIPAYSANIPAGVLQLHSGHYRNPS
                     QLPAGAILVVGAAQSGMQIAEELYQSGRTVYLATGFAPRAPRRYRGRETFSWLNETGF
                     VSTPVEKLPSPKARFISNPQLSGKDGGHSLNLHQFARDGVKLLGHIAGAQDGKIMFKA
                     DLKENLAKTDKTEKDILAMIDKYIETNGINAPQETIPELQDGYASEEITELDLKAENI
                     NTVIWAIG"
     assembly_gap    1068..1167
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1199..2332
                     /locus_tag="PL000_22070"
     CDS             1199..2332
                     /locus_tag="PL000_22070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC91628.1"
                     /translation="MTKKRVVVLGAGFGGLELSTILSDALGDELDLTLIDRNDSFYFG
                     FSKFDVMFGNKTPDAVRIAYRNIDKRGVIFRQETVKAIDPVTRRVTTDKATYEADVLV
                     IALGADYDIAHTPGLAEAGNEFYSFAGAERLRSVLPAFNKGRVIVGVTSTPFKCPPAP
                     SEAALLTHDYLTERGIRADCEISLVMPFGIPIPPSPETSAALMAAFKERGIRFVPNRL
                     VRALDGARRVAILDNGDEMPYDLFLGVPKHRVPDVVAESGMTEDGWIPVDTKTLKTRF
                     ENVYAIGDVNSVGTPKAGVFAEGAARTVAAEIIAELKGGHADAYRGIGACYIEFGAKR
                     VGKVQVEFLAGPPTGSYTEASSALVDEKKEFGASRRAKWFGLG"
BASE COUNT          590 a          579 c          616 g          496 t
ORIGIN      
        1 cgtgttgtaa ccatcatatt atcggtaaag gagcacggca tggaatcaat cgaaaccatc
       61 atcatcggcg gcgggcaggc gggacttgca accagttacc atttgaaaca acttggacgt
      121 gaacacatca tcttcgaagc ggcggatatc cccgcccatg tctggcgcga tgaccgctgg
      181 gactcattca ccttcgtcac ccccaactgg gcgatccaac tccctggtgc tggttatgaa
      241 gggcgtgacc ctgatggctt tctaaaaaaa gatgagatcc ttgcctattt tgaaggctat
      301 gtcgaaaaat atcaattccc tattcaatta aacacaaccg tgaccgaagt cgcacccgtc
      361 gaaggcgggt atcaagtcat tgccaatgga aagacctacc acgctaaaaa tatagtgatg
      421 gcaacaggtt cctttcagaa gccgaagatt cccgcgtaca gcgcaaacat cccagcaggt
      481 gtattgcaac tccattcagg tcattaccgc aatccgagtc aactgcctgc gggcgctatc
      541 cttgtcgtcg gcgcggcaca gtcgggcatg cagatcgccg aagagctata tcaaagcgga
      601 cgcacggtct atctcgcgac aggttttgcg ccgcgcgcgc cgcgccgtta tcgcggcagg
      661 gaaacgtttt catggcttaa tgaaacgggg ttcgtttcca cacctgtgga aaaactcccg
      721 tcccctaaag cccgttttat cagcaatccg cagctcagtg ggaaagacgg tggtcattcc
      781 ctcaacctgc atcagtttgc gcgtgacggc gtgaagctcc tcggtcacat tgcaggcgca
      841 caggatggca agatcatgtt caaagccgac ctgaaagaaa accttgcgaa aacggacaaa
      901 accgaaaaag acatccttgc catgatcgac aaatacatcg aaacgaacgg catcaacgcc
      961 ccgcaagaaa ccatccctga attgcaggat ggctatgcct ctgaagagat caccgaactc
     1021 gacctgaaag cagagaacat caacaccgtc atctgggcga tcggctannn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnaag accatttatt aataacaagg agagatttat
     1201 gacaaagaaa cgagtggtgg tgttaggcgc tgggtttggc ggattggaac taagcacgat
     1261 cttgtccgat gctctgggtg acgagctgga cctgacattg atcgacagga atgattcttt
     1321 ttattttggc ttttcaaaat ttgacgtaat gttcggcaac aaaacacccg atgcggtacg
     1381 cattgcatat cgcaatatcg acaagcgtgg cgtgatcttt cggcaggaaa cggtcaaagc
     1441 cattgaccct gtaacgcggc gtgtgacgac cgataaagca acatacgaag ccgatgtgct
     1501 ggtcatcgct ttgggcgccg attacgacat tgcccacaca cctggccttg cagaagcggg
     1561 caatgagttc tattcgtttg ctggagcaga gcggttgcgc agtgtactgc ctgcgttcaa
     1621 caaaggacgc gtcattgtgg gcgttacctc aaccccattc aaatgtccac ctgcgccgag
     1681 tgaagccgcg ttattgacac atgattattt gacagaacgc ggcatacgcg cagattgcga
     1741 gatcagtctg gtcatgccgt ttggcattcc gatcccgccc tcgcccgaaa cgtcagcggc
     1801 gctaatggct gcattcaaag agagaggcat ccgcttcgtt ccgaatcggc tcgtccgcgc
     1861 attggatggt gcccgacggg ttgccattct cgataacggg gatgaaatgc catacgatct
     1921 cttcctcggc gttcccaaac atcgtgtgcc agatgtggtc gccgaaagtg gaatgacgga
     1981 agatggttgg attcccgtgg atacaaagac attgaagacg cgtttcgaaa acgtgtatgc
     2041 catcggcgat gtgaacagcg ttgggacacc caaagcaggt gtgttcgccg aaggcgcagc
     2101 gcggacggtt gccgctgaga tcatcgctga gttgaaaggc ggtcatgcag atgcctatcg
     2161 cggcataggg gcatgttaca ttgagtttgg agcaaaacgt gtgggtaaag tgcaggtgga
     2221 attccttgct ggtccaccca caggaagtta taccgaagca tcgtccgccc tggtggatga
     2281 gaagaaggaa ttcggcgcaa gccgcagggc gaagtggttt gggctgggat aagattcatc
     2341 tggtccgatt acgagaccat gatggaagaa aaattcggga a
//
LOCUS       DAOQLC010000305         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_28443_length_2365_cov_19.291775, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000305 DAOQLC010000000
VERSION     DAOQLC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28443_length_2365_cov_19.291775"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1344
                     /locus_tag="PL000_22075"
     CDS             145..1344
                     /locus_tag="PL000_22075"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNC91629.1"
                     /translation="MMLADLESTRLNVENKTRSNLFSWRGQFTPQFVDYLLETYARPG
                     QVVLDPFCGSGTVLQEATSRRLMAVGLEINPAAYAMAKFFSLSTLSAGARNALAEEAT
                     GLLRTSLAHLPQDTPLFTDSEDYRQRVSNLLHLATELLSGTDDKQTRLLLLLSLFRAE
                     SSRNGGLASVVSRSFQFVLEQLLQLPRTERTSVASLSDARNAHLHLDEEAHVIITSPP
                     YINVFNYHQNYRALLELIGFDMLKVAHSEMGSNRKHRGNRFLTVIQYSLDIELAIKSF
                     ARALVPNGLLIMVLGRESKVRGAAFGNSEIVASLIERSGAFARPKSHERVFTNRYGQS
                     IYEDILVAQRIGSPDESEQARGVAKTSLLAALKDQVGDIRANIEEAIGLIDSVRPSPL
                     FNKAALI"
     gene            1348..2241
                     /locus_tag="PL000_22080"
     CDS             1348..2241
                     /locus_tag="PL000_22080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bpu10I family restriction endonuclease"
                     /protein_id="HNC91630.1"
                     /translation="MANTPHKEKLTAAIANPKCDADDVALLNEALEAYEDWSSKLSGL
                     TTTGRKRIEDMTSLLNSYKDFLEVDLIAKRGTAFIKRQKGQLKLDNSVIEEFLVHLAT
                     DKVLSGLPDFRLEVGPQTAFMSLAFTPSSLNALGGKPAVTLKEKDTDFALGKTIHYQF
                     STEPTFPTKQTSQGKLVLAVLATECKINLDKTMFQEAAGTAARFKQGCPMSRYYLLVE
                     YLDMEPEDCRLTALDNVFLLRHTKRLPFEKRGVYAEVKRQHDSYPIDAEVVWRFVNEL
                     QGFVDAVWYDPQKALQRGSFI"
BASE COUNT          562 a          666 c          602 g          535 t
ORIGIN      
        1 tgcggctgga cctggtgctt tcggcgcggc agataaacgc tattcgttgg gcgtccctag
       61 ccttgcccag ccttgcccag tagtcgctaa cggccattga tgggaaaata gcgccacgaa
      121 ccacgaaccc cggcgaaatc cagaatgatg cttgctgacc tcgaaagtac gcgccttaat
      181 gtcgagaaca agacaaggtc aaacctcttt agctggcgag gccagttcac tccgcagttc
      241 gtagattatc tgctcgaaac ctacgcacgc cctgggcaag tggtcctaga cccattctgt
      301 ggaagtggga cggttttgca ggaagccacc tctcgtcgcc taatggccgt gggactagaa
      361 atcaatcctg ctgcctatgc gatggcaaag ttcttttctc tgtcgacatt gagtgcaggc
      421 gcccggaacg cattggcgga agaggctacc ggattactcc gcacctcact cgctcatctg
      481 ccgcaggaca ctcccctttt cacggattca gaagactacc ggcaaagggt ttccaacctt
      541 ctgcatctcg cgacggaatt gcttagcggg actgacgaca agcaaacgag gcttctactt
      601 ctcctctccc tatttcgagc agagagttca aggaatggtg gcttggcgtc agtagtcagt
      661 cgctcgtttc agttcgtact agagcagctt cttcagcttc ctcgtaccga acgaacatcc
      721 gtcgcatcgc tctccgatgc gagaaatgcc catcttcatc ttgatgagga ggcgcatgtc
      781 attattacga gccctcccta cattaacgtc tttaactacc accagaacta tcgcgcccta
      841 ctggagctaa ttggttttga catgctcaaa gtagctcaca gcgaaatggg ctcaaaccga
      901 aaacaccgtg gaaatcgctt tctgacggta attcaataca gcctggatat tgaactggca
      961 atcaagagct ttgctcgggc gcttgttcca aacggcctac taattatggt gcttgggcgc
     1021 gagtctaagg ttcgtggcgc tgctttcgga aactcagaga ttgttgcatc tctcatcgag
     1081 cgcagcggtg cgtttgcaag gccgaagagc cacgagcgcg tgtttaccaa ccgctacgga
     1141 cagtccattt atgaagacat acttgtcgct caacgcatcg gctcgccaga cgaaagcgaa
     1201 caagcaagag gcgttgcaaa gacatcccta ttggccgcac tgaaagacca agttggcgac
     1261 atacgggcca acattgaaga ggccattggc cttattgatt cggttcgccc atcccctcta
     1321 ttcaacaaag ctgccctaat ctgaccaatg gcaaacactc cacacaaaga aaaactcaca
     1381 gccgctattg ccaatccgaa gtgtgacgcc gacgatgtgg cgctgttaaa cgaggcgctt
     1441 gaggcttacg aggactggtc gtcgaagctc tcagggctca ccactaccgg caggaagcgg
     1501 attgaggata tgacaagtct cctcaattcc tacaaagatt tcttggaagt cgacctgatt
     1561 gccaagcgtg gcaccgcgtt tatcaagcga cagaagggcc aactgaaact cgacaactca
     1621 gtcatcgagg aattcctcgt acatctagcc actgacaagg ttctttctgg tcttcctgat
     1681 tttcggcttg aggtcggccc ccaaacggcg ttcatgtcgc tggccttcac tccctccagc
     1741 cttaacgccc ttggcggtaa gcccgccgtc acgctgaagg aaaaggatac tgactttgcg
     1801 ctgggcaaga cgattcacta tcagttctca accgagccca catttcccac gaagcaaacc
     1861 agccaaggaa agcttgttct tgccgttctt gctactgaat gcaagatcaa cctcgacaag
     1921 acgatgtttc aagaggctgc gggaactgcg gcgagattca aacaaggatg tccgatgtcc
     1981 cgctactact tgctggtgga atacttggac atggaacctg aagactgccg cctcaccgct
     2041 ctcgataacg tctttctact tcgccacacg aaacgtcttc cattcgagaa gcgtggcgtc
     2101 tatgcggaag tgaaacgcca gcacgatagc tatccaattg atgcagaagt cgtgtggcgg
     2161 tttgttaatg agcttcaggg gtttgttgat gccgtatggt acgacccaca aaaggcgtta
     2221 cagcgcggct cgttcattta gccccggtac gcccaaccca tcggtccacc ggacgctgcg
     2281 cgatgaagcc gcgcagcgcc ggtgacctca aacgttgggc gctcgcgggg gaaagcgcgt
     2341 gagtcttcgt tgcggtcgcg gccgt
//
LOCUS       DAOQLC010000306         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_28785_length_2350_cov_2.926797, whole genome shotgun sequence.
ACCESSION   DAOQLC010000306 DAOQLC010000000
VERSION     DAOQLC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28785_length_2350_cov_2.926797"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PL000_22085"
     CDS             <1..1242
                     /locus_tag="PL000_22085"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC91631.1"
                     /translation="ERLIKINKKQLTLSDSAFRQRVITTISRVQAAYWDLVFAIRNSQ
                     ISREAVELADVTLRNNLRQVEVGTLAPIEIVSARADLESRKDTAISALQQVTIAENAL
                     KALILGDPLADQWNAVIEPTEKVEFTAIDITLDDAIKSALQNRPEVEQIKLRQELKNV
                     DLQFFRNQEKPQVDFVASYILSGLAGQTSLPVVDSRSMSPQSAANQLQSSIYNFNQQL
                     GQPSLINPVTRQTQGGIPSRFVGGYGTALSNVFSFDFRSYNLGVNFSFPLRNRTAKAN
                     IGRTLAENRQLQAQERQTVQTIQAEVRNAVQAVLSARQRYEAARAARIAAKEQLEGEQ
                     KKYEAGISNNFFVLDRQTRFSNAQGQEIRALTDYNKSISELQRVTSTTLSVNNIQVTS
                     NPDQAPNQPAGTTDSPSRTND"
     gene            1410..2141
                     /locus_tag="PL000_22090"
     CDS             1410..2141
                     /locus_tag="PL000_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91632.1"
                     /translation="MPITHLILIACHAVYLGRTQDETLLDQSWCLQTFQFGEPVFYIE
                     HIEAGISLAAQNPDSLLIFAGGQTRREAGPLSEAQSYWLLAEQSGWWERTEVRDRATT
                     EEFSRDSFENLLFGICRFREYTGKYPHRISVVSWKFKQERFDLHRQAIRFPADRFEFI
                     GVNNPPDLESALIGEHQNSLIPFSQDPFGTGPVLAAKRHHRNPFHRQHGYTTSCPEVA
                     LLLNDAGPDPFPDNLPWEKHNGHAT"
BASE COUNT          612 a          657 c          555 g          526 t
ORIGIN      
        1 gaacggttga ttaagatcaa caaaaagcaa ttgacgctca gtgatagtgc tttccgccaa
       61 cgtgtcatca ccacgatttc acgcgtccag gcggcttact gggatctggt gtttgctatc
      121 cgcaatagcc agatttcccg cgaagccgtt gaactggccg acgtcacgct gcgcaataac
      181 ctccgtcagg ttgaagtcgg cacgctggcc ccaatcgaaa ttgtctcagc ccgcgccgat
      241 cttgagagcc gcaaagacac cgccatttcc gccctccagc aagtgaccat tgcagaaaac
      301 gcactcaaag cattgatttt gggcgacccg ctggctgatc agtggaatgc ggtgattgaa
      361 ccaactgaaa aagtggaatt tacggcaatt gatattaccc ttgatgacgc catcaaaagc
      421 gccttgcaaa atcgcccgga agtcgaacaa atcaaactgc gccaggaact gaagaacgtt
      481 gacctgcaat tcttccgcaa tcaggaaaaa ccacaggttg attttgttgc cagctacatt
      541 ctgtctggat tggccggtca aacgtcgctg ccagtggttg attcacgttc gatgtcaccg
      601 caatcagccg ccaatcaatt gcagtcatca atttataatt tcaaccagca acttggtcag
      661 ccctcgctca tcaatcccgt cacccgacag actcaggggg gcatcccaag tcgctttgtg
      721 ggtggatatg gaaccgccct gtcaaacgtc ttttcatttg atttccgaag ctacaacctg
      781 ggtgtgaatt tcagctttcc actgcgtaac cgaaccgcca aggccaatat tggtcgtacc
      841 ctggctgaaa atcgccagct acaggctcaa gaacggcaaa ccgttcagac cattcaagct
      901 gaagtccgca atgccgtaca ggcggtgctc tcagcccggc aacgctatga agccgcccgc
      961 gcggctcgca ttgcggccaa agaacagctt gagggcgaac agaaaaaata cgaagccgga
     1021 atctcaaaca acttctttgt gctggatcgc cagacgcgat tttcaaacgc ccagggacag
     1081 gaaatccggg cgctcactga ctacaataag agcatttccg aattacagcg cgtgacttcg
     1141 accaccctgt cagtcaacaa cattcaagtc acgtcaaacc cagaccaggc tccaaatcaa
     1201 ccggctggga ccaccgattc accgtcacgt accaatgatt aaaataaacc tctgagagta
     1261 tcccttcttt ttgtttgttc tcccctttaa cctcaaagta tcctccgctc tacgtgaacg
     1321 gaggattttt tttgccttgg atatcagaaa caatacctgg tatctgactt tgataacctc
     1381 gaatcccacc ccctgtccct taatgcccaa tgcccatcac tcacctgatt ctaattgcct
     1441 gccacgcggt ttatcttggc cgaacccagg atgaaaccct gcttgatcaa agctggtgtt
     1501 tgcagacatt ccagtttggc gagccagttt tttatattga acatatcgaa gctggtattt
     1561 ccctggccgc ccaaaaccct gactcactgc tgatcttcgc tggtggtcag acccgccggg
     1621 aagccggccc cctgagcgaa gcccaaagtt actggttgct ggctgagcaa tctggctggt
     1681 gggaaagaac tgaggtcaga gaccgggcca caaccgaaga gttttcacga gattccttcg
     1741 aaaaccttct ctttggcatc tgtcgctttc gtgaatatac aggcaagtat ccacaccgga
     1801 tttcggtggt cagttggaag tttaaacagg agcgatttga tcttcaccgg caggcaattc
     1861 ggtttcccgc agacagattt gaattcatcg gtgtcaacaa tccacctgac ctggaatcag
     1921 ccctgatcgg cgaacaccaa aacagcctga ttcctttttc ccaggatccc tttggcaccg
     1981 gcccggtgct ggccgccaaa cgtcatcatc gcaacccatt tcaccgccag cacggctaca
     2041 ccacaagctg cccggaagtg gccttgctcc taaacgatgc cggaccagac ccatttccag
     2101 acaaccttcc ctgggaaaaa cacaacgggc acgccacctg agtgacgtgc ccgttgggga
     2161 gatcagggtt tagggtttag ggtttagggt ttagggttca gggtttaggg tttagggttc
     2221 agggtttagg gtttagggtt tagggaaaag acaaaaggga caaaaaggac gaaaaggaca
     2281 aaaaggacaa aaaaggattt cgaaaacccg gaacccggaa cccggaaccc ggaacccgga
     2341 acccggaacc
//
LOCUS       DAOQLC010000307         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_29401_length_2324_cov_24.176730, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000307 DAOQLC010000000
VERSION     DAOQLC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29401_length_2324_cov_24.176730"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..445)
                     /locus_tag="PL000_22095"
     CDS             complement(26..445)
                     /locus_tag="PL000_22095"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC91633.1"
                     /translation="MSKEKFAENLLFKKGQNMNVFVLVGRIADNADLYEQPNGKKVIN
                     FRVEENRPRFKNDPPVFWQCVAWNATAVEMGKRCRKGRSFTLHGELKQGKNKMGEPVL
                     KMDVKVFIEHPQPEKEITSDDVTAYRSAVSYAERYAD"
     gene            484..888
                     /locus_tag="PL000_22100"
     CDS             484..888
                     /locus_tag="PL000_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91634.1"
                     /translation="MSFKHKITFKLTETSENQKENDLVLPLPVVDYDIQKKLILRDLI
                     RDTDNLRLIDVPQSVYGIPKYLFIFAVYTVDNSLIGAVKGKPAPFKVRLNGDAVSKDI
                     TSGVLFWGGEVSSLYIETPVTDYIEYQVFLGA"
     gene            892..1788
                     /locus_tag="PL000_22105"
     CDS             892..1788
                     /locus_tag="PL000_22105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91635.1"
                     /translation="MATFSGYLNDQSEDYDYKDLGLKLTRGENPLAAPDPKKWGCLVH
                     PDELRQVIFIGNGRLITAGGDQITNYHLKNWTDQTVRQLAEHLQWDIYPRLWRHRPFH
                     EQERQIEDYAEWDDCYDYVHKSSELFMVKLRRQPVSKVIAWKLVSPYNTQVMLDLKQK
                     MIPKYAGGILKSAIYVGGSAFQMSIAQYRASIYKQDLPSAYFVDYVSGYDHADRVPRE
                     FREVVQKLMTINVMSAFGDGIVSGMANYSISLGPLSESIGTTMSSTSSFYGARIQQLQ
                     GELKEWFKVRGGAYRGIKFAGL"
     gene            complement(1814..2158)
                     /locus_tag="PL000_22110"
     CDS             complement(1814..2158)
                     /locus_tag="PL000_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91636.1"
                     /translation="MLFSRSKRFNTKLQKLKKDSESKRQVDADNRSDETAQDAGADRQ
                     TQSLIFGVFGFSNRRRRDIDGTLATLLDCLVRAGIFKDDSLDFIQAESFTGIRGQRDF
                     VEIIIIEGNNGI"
BASE COUNT          628 a          539 c          482 g          675 t
ORIGIN      
        1 agaccggaat acaggttaag tattttcaat ctgcatacct ttcagcgtaa ctgactgcac
       61 tcctgtaagc ggtcacatcg tctgatgtaa tttccttttc cggctgaggg tgttcaatga
      121 acacttttac gtccatcttt agaaccggtt cacccatctt atttttgccc tgcttcaatt
      181 caccgtgcag ggtaaagctt ctgcctttcc tgcatcgttt ccccatttca actgccgttg
      241 cgttccatgc cacacactgc cagaatacag gcggatcatt tttgaatctt ggccggtttt
      301 cctctactcg gaaatttatc accttcttgc cgttcggctg ctcatacaga tctgcattgt
      361 ccgcaatcct tccaactaac acaaatacgt tcatattctg tcctttctta aaaagtaggt
      421 tttctgcaaa tttttcttta ctcataataa aaaaaactca aaaattaaac agggagaaaa
      481 taaatgtcct tcaagcacaa aattacattc aaacttactg aaaccagcga aaaccagaag
      541 gaaaacgatt tagtccttcc tctgccggtt gtcgattacg acattcagaa aaaactcatt
      601 ctcagagacc tcatccgaga cactgacaat ctcagactca ttgacgttcc tcagtccgtt
      661 tacggcattc caaaatacct gtttatcttt gcagtctata ctgtagacaa ttcactcatc
      721 ggagctgtca agggcaagcc tgcacctttc aaggtccgtc tgaacggcga tgccgtaagc
      781 aaggatatta catccggtgt tttgttctgg ggaggggaag tttccagtct gtatattgaa
      841 acacccgtga cagattacat cgaataccag gtatttctcg gagcctgata aatggcaact
      901 ttttccggtt atctgaatga ccagtctgag gactatgatt acaaagacct tggcctgaaa
      961 ctcacacgag gggaaaaccc gcttgcggct cccgacccga aaaaatgggg gtgtcttgtt
     1021 caccccgatg aactcagaca ggtcatcttt atcggaaacg gacgtctgat tacagcgggc
     1081 ggggatcaga taaccaacta tcacctgaag aactggacgg atcagacagt cagacagctt
     1141 gccgaacatc tgcaatggga tatttaccct cgtctttgga gacaccggcc ttttcatgaa
     1201 caggaaagac agattgagga ctatgcagaa tgggatgact gctatgatta cgttcacaag
     1261 tccagcgaac ttttcatggt caaacttcgc agacagccgg tctcaaaggt catcgcatgg
     1321 aaacttgtaa gtccatacaa tactcaagtc atgcttgacc ttaaacaaaa gatgatcccg
     1381 aaatatgcag ggggaattct aaaatctgcc atttacgtcg gcgggtctgc ttttcaaatg
     1441 tcgattgctc agtatcgtgc ctcaatttac aagcaggatc tgccgtcagc ttattttgta
     1501 gattacgttt caggttatga ccatgctgac cgagttccta gagaattcag ggaagttgtg
     1561 cagaagctga tgactatcaa tgtaatgtcg gcttttgggg acggtatcgt ttccggtatg
     1621 gcaaactatt ctatttcgtt gggacctctt tcggagtcca taggaactac aatgagttcg
     1681 acttcgagtt tttacggtgc aagaattcag cagcttcagg gagaactgaa agaatggttc
     1741 aaggtgcgag gcggggcata tcgcggaatc aaatttgcag ggctttgatt ctgtctgcat
     1801 tcagaatgaa taatcagatt ccattattcc cttctataat tatgatttcc acaaaatccc
     1861 tttgtcctct aatgcctgta aaactttctg cttgaatgaa gtccaagctg tcatctttga
     1921 agattccggc tctaacaaga cagtcaagta aggttgcaag agtgccgtca atgtctcgac
     1981 gtcggcggtt agagaacccg aaaactccaa agatgagaga ctgagtttgt ctgtcagctc
     2041 ctgcatcttg cgctgtttca tcggatcggt tatcagcatc gacctgcctt ttactctcac
     2101 tatctttttt gagtttttga agcttggtat tgaatcgttt agatcgactg aatagcattt
     2161 aattgtccat ttcatccctt tcgcctttca agtattgttt ctctcagacg tctcatttcg
     2221 tctttcagat cccgcatgtg aacaagcagg aaatagcaca tacttatcag catcactgtc
     2281 agaaagaagt cagtgaagac cgatatgaac ttgactgtcc agta
//
LOCUS       DAOQLC010000308         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_29924_length_2301_cov_20.913624, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000308 DAOQLC010000000
VERSION     DAOQLC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29924_length_2301_cov_20.913624"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..923)
                     /locus_tag="PL000_22115"
     CDS             complement(54..923)
                     /locus_tag="PL000_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNC91637.1"
                     /translation="MNKVNHFFRRTSFGSTVTLLILVAMWSAPTLGLLITSLRPRDLA
                     EVSPWWEAFAAPFSTPWTFEAYQNSLASGMLDSLVNTIAVAVPSTILPLMIAANAAFA
                     FTFLNFKGKEFFFALLVALMVIPLQSSLIPVLRGMVWMQKTFDIELTGTYVSAWIVHS
                     AFSMPLAIYTLRNYMMTLPKELIEAATVDGASYYQIFWKLVLPMSTPAIASFAIFQFL
                     WVWNDYLIAFIFVGEQKAVMTYRLLRLLGQYGEGWQDVAAGSFVSLIIPLIVFFSLQR
                     YIVRGLLAGSVKG"
     gene            complement(920..1975)
                     /locus_tag="PL000_22120"
     CDS             complement(920..1975)
                     /locus_tag="PL000_22120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNC91638.1"
                     /translation="MSDLIFGLVSIVIGVAASYGVFWLLNFLLSLLPERIYRKVNWIA
                     FLLPAALLVILVLVAPLFQTVIWSFKSDNVKEWVGFQNYVEIFTDEVKPDKIETKKEE
                     GAVFVADDRILDDRFMDILINNFLWVLVVPAATVVVGTAAATLTNQVGPKRERVFKSL
                     IFMPMAISFVAASTIWAYFYSFVPPGRPEVGLLNALVKFFGGEAQPWLTIDKGRLNSF
                     LLMVVIIWLQGGYAMVVISAAIKNVPAETLEAAQLDGANSWQVFFRVVLPQVWGTVMS
                     VFVTILIMVMKIFDIVLSMTRGNFTTSVLAFEWYKEFFENSNLGPASAVVTILVVLIA
                     PLMWLQIRTVRQQEALR"
     gene            complement(2111..>2301)
                     /locus_tag="PL000_22125"
     CDS             complement(2111..>2301)
                     /locus_tag="PL000_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91639.1"
                     /translation="LYPNEMQKKIGEAMAAAAVFGFDGSDQMPAEVNAEFWAAGTDFV
                     AGRITWAEAAERIDSKYP"
BASE COUNT          678 a          587 c          521 g          515 t
ORIGIN      
        1 tgattcatat aaaaagtgga ctggttgccc agtccacttg tccttttgga aagttatcct
       61 ttgactgagc ccgctagcaa cccgcgtacg atatagcgtt gcaaactgaa gaacacgatc
      121 aacggaatga tgagggagac aaaagagcct gctgctacat cctgccagcc ttcgccatat
      181 tgacccaaca gcctgagcag acgataggtc attaccgctt tctgttcgcc tacgaagatg
      241 aaagcgatca ggtagtcatt ccatacccag aggaattgga agatcgcaaa agaagcgatc
      301 gccggggtag acatgggcaa gaccagtttc caaaagatct ggtaatagct ggcgccatct
      361 accgtggcgg cttcgatcag ttccttgggc agggtcatca tgtagttgcg cagggtatag
      421 atcgctaatg gcatggagaa ggcactatgc acgatccatg cactgacata ggtgccggtt
      481 aattcaatat caaatgtttt ttgcatccag accattccac gcagtactgg aataagagag
      541 gactgcaagg gaatcaccat caatgccacc aacaatgcaa agaaaaactc cttgcctttg
      601 aaattcaaaa aggtgaatgc aaatgcagca ttggcagcaa tcatcaacgg cagaatggtg
      661 gatgggactg caactgcaat ggtgttgacc aacgagtcca acatgccgct ggcaagtgag
      721 ttctgatagg cttcaaacgt ccatggggtg ctaaatggtg cggcaaatgc ctcccaccat
      781 ggagagactt cagcaagatc acgcggacgc aaggatgtga tcaacaaacc aagtgtagga
      841 gcggaccaca tcgcaaccaa aataagcaat gtgacggtac tgccaaaact ggtccttcta
      901 aagaaatgat taaccttgtt catcgcaaag cctcctgttg tcgtaccgtt cgaatctgca
      961 gccacatcaa aggtgcgatc aacacaacca gaatggtcac taccgcagag gcgggaccca
     1021 ggttggaatt ctcgaagaac tctttgtacc attcaaaggc aagcacactc gtggtgaagt
     1081 taccacgagt catggagagc acgatgtcga atatcttcat aaccatgatc aggatcgtca
     1141 cgaagacaga catgaccgtt ccccaaactt gcggaagtac cactctgaag aaaacctgcc
     1201 atgaattcgc accatcaagc tgtgcagctt ccagagtttc tgcaggtaca ttcttaatgg
     1261 ctgctgaaat caccaccatg gcatacccgc cctgcagcca gataatcacg accatcaaca
     1321 aaaatgaatt caaacgtcct ttgtcaatcg tcagccaggg ctgagcttct cccccaaaga
     1381 actttaccag cgcatttaat aaaccaactt ccgggcgtcc cggtggaaca aacgaataga
     1441 aataagccca aatggtcgaa gccgctacaa agctgatcgc catcggcata aaaatcaagg
     1501 atttaaagac tctctcgcgt ttcggtccca cctgattcgt aagggtggct gctgctgttc
     1561 ccaccaccac tgtggctgct ggaacaacca ggacccataa aaagttattg atcaggatgt
     1621 ccataaaacg gtcatccaaa atacggtcat ccgctacaaa gacagcgcct tcttcttttt
     1681 ttgtttcaat cttgtcaggt tttacttcat ccgtaaagat ctctacatag ttttggaacc
     1741 caacccattc cttgacgttg tcactcttga acgaccagat cactgtttga aaaagcggag
     1801 cgacaagaac aaggatcacc aacagcgcag ctggtaataa aaatgcaatc caattgactt
     1861 tgcgataaat gcgctctggc agcaaagaaa gcaggaagtt aagcagccaa aataccccat
     1921 aactggctgc cacaccgatc acaatggaca ccaaaccaaa gattaaatcg ctcatttctc
     1981 aatctccatt ttttgggttc gttaccggcc ttgagccgag tattttttat tgagcgagtg
     2041 ctaaaagatt gtggcaccga ccccagaagt tcaagagccg gtgccactat tttacctagt
     2101 tacagaacga ttacgggtac ttgctatcga tgcgttcggc agcttcagcc caagtgatgc
     2161 gaccggcaac aaagtcggta ccggcagccc agaactcggc gttcacttca gcgggcatct
     2221 ggtcggaacc atcgaaaccg aacacagcag cagcagccat ggcttcaccg atcttcttct
     2281 gcatttcgtt cgggtagaga g
//
LOCUS       DAOQLC010000309         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_30665_length_2271_cov_25.091606, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000309 DAOQLC010000000
VERSION     DAOQLC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30665_length_2271_cov_25.091606"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            374..1081
                     /locus_tag="PL000_22130"
     CDS             374..1081
                     /locus_tag="PL000_22130"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC91640.1"
                     /translation="MGLADLHLHTIYSYDGTAPLPAVLTRAKQLGLDVIAITDHDEIA
                     GALKALEIAPLYGVEVIPGVEITTAEGDLLALFIKEKVAAGLSLVETVLKVRELGGVC
                     IAPHPMAGGMGMKSLNAMSILKALRNRKVAETLIGIETYNGTSIDRVSNHSASLLSNR
                     LKLARTGSSDAHILETIGFGATEFEGKTAADLLAALKNRTTKVRKQNEWGMFKILGSW
                     ALNYLGSLFARRAITTN"
     gene            1095..1244
                     /locus_tag="PL000_22135"
     CDS             1095..1244
                     /locus_tag="PL000_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91641.1"
                     /translation="MLIFCIALYLVVIVPLLALLWASLVVAKWDDKDRGYEILDEQEF
                     LELAL"
     gene            1259..2095
                     /gene="surE"
                     /locus_tag="PL000_22140"
     CDS             1259..2095
                     /gene="surE"
                     /locus_tag="PL000_22140"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC91642.1"
                     /translation="MTKNILVTNDDGVLAPGLLALAQEMRKLGAVTILAPDRNWSGGG
                     HVKTLDRALRVREFHLSDGTQAFGSDGAPSDCVALATLGYFKEKFDLVVSGINVGANL
                     GHDVTYSGTVTAAMEAVIAGLPGVAVSLEIPEGHVGPIDFGPSALAAGMAVRNVLQHG
                     LPADTLLNVNVPFLKQEEIQGFRMTRQGLRVYHSRLDERVDPRGRPYYWIGGEAPTGV
                     PESGTDVGALSDGFVSVTPLQLDLTAYRAVSEINTWNWHDPILSEVSLLGPSSVVAAE
                     NQ"
BASE COUNT          537 a          574 c          601 g          559 t
ORIGIN      
        1 aaatgttaac tggctcttaa gtcgaaaata aaggctttga agtatgattt cagtatagga
       61 acagaaacat atggaaacca ttttatcttc tgttattttt gtatccatgg tcctcgtctt
      121 tggggtggat gcgacccgca agcgccgcga ctacctgaag aaacgcagcc gtaactgaca
      181 ttggttttct cctcaccttg cctccacgag gcaggccgcc cgccgtcatg catgatggcg
      241 ggcggctttt gttaacacaa cccatacaat ttcttaaaac gatcttcaca gcagcttaat
      301 ggtccacaga tacactgtgg aaatgaaaag acatgtgaca tggacttcaa tgtccatttt
      361 taggagaaat tgaatgggac tcgcagattt gcatttacac acgatctaca gctacgatgg
      421 cactgccccc ctgcccgctg tcttgacccg tgcgaaacaa ctggggttgg atgtcatcgc
      481 cattaccgac catgatgaga ttgcaggtgc cttgaaagcc ctggaaattg cacccctgta
      541 cggggtggaa gtgatcccgg gcgtcgaaat caccacggct gaaggcgact tgctggccct
      601 cttcatcaaa gaaaaagtag ccgctggcct ttcacttgtg gaaacggttt tgaaggtgcg
      661 tgaactcggc ggggtatgca ttgcgccgca ccccatggca ggcggaatgg ggatgaagag
      721 tttgaacgcc atgtccattt tgaaagccct gcgcaaccga aaggtggctg aaaccctgat
      781 cggaattgaa acctacaacg gcacctccat tgacagggtc agcaatcact cggccagcct
      841 gctttcgaac cgcctcaaac tcgcgcggac aggcagcagc gacgctcaca tcctcgaaac
      901 catcggtttc ggagcaactg aattcgaagg caaaacagcc gccgacctgc tggccgcttt
      961 gaagaaccga accacaaagg tccgcaagca gaatgagtgg ggcatgttca aaatactggg
     1021 cagttgggca ttgaactacc ttggcagcct ctttgcacga cgagcgataa cgacaaattg
     1081 aagggtaggt tgagatgttg attttttgca ttgcgctgta tttggtggtg atcgtacccc
     1141 tgcttgcatt gctatgggct tcattggtcg ttgccaaatg ggacgataaa gaccgtggct
     1201 atgagatttt ggatgagcag gaatttttgg aattggctct ttaagaagaa tggatagaat
     1261 gacaaagaac atactggtta caaatgatga tggtgtgctt gcgcccggac tcctggctct
     1321 ggcacaggaa atgcgtaaac ttggcgcagt gacgatcctt gcccccgacc gaaactggtc
     1381 tggcggcggt catgtaaaaa cactggaccg tgccttgcgt gtgcgcgagt ttcatctcag
     1441 cgatggtacg caggcttttg gtagtgacgg cgccccttcc gattgcgtgg cgctggcaac
     1501 cctgggatat ttcaaggaga aatttgacct ggttgtttcg ggcattaatg tcggtgcgaa
     1561 cctgggccac gatgtgacct actccggaac cgtaaccgct gcaatggaag ccgtgatcgc
     1621 agggctgccc ggtgttgctg tttctttgga aatacccgaa gggcatgtcg gcccgatcga
     1681 ttttggacct tccgctcttg ccgcagggat ggctgttcgg aacgtcctgc agcatgggtt
     1741 gcccgctgac acattgttga acgtcaatgt accgttcctc aaacaggaag agattcaagg
     1801 ttttcgtatg acacgacaag gcttgcgcgt gtatcacagc cgactcgatg aacgtgtgga
     1861 cccgcgcggc cgtccctatt actggatcgg cggggaagcc ccaacaggtg tccctgagtc
     1921 gggaaccgat gtcggcgctt tatccgatgg atttgtttcg gtcaccccgt tgcagcttga
     1981 tctcaccgcc tatcgcgccg tctcagagat caacacatgg aattggcacg accccattct
     2041 ttctgaagtc tcgttgctgg gaccttcttc tgttgttgcg gcagagaatc aatagcagcg
     2101 ttcttctcct ccttcgtgtc tccagatcgg agacggtgtc caccagaaat ggtggacatt
     2161 ttgtttaacg ccctcgtccc aagccttaac aagacgttaa cccattcttc cattgacctt
     2221 aagagccgct tgttatcatc ctcatcgtta tataaaacat aggagaatag a
//
LOCUS       DAOQLC010000310         2231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_31656_length_2231_cov_13.319393, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000310 DAOQLC010000000
VERSION     DAOQLC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31656_length_2231_cov_13.319393"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PL000_22145"
     CDS             complement(<1..370)
                     /locus_tag="PL000_22145"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNC91643.1"
                     /translation="MTIETILPTPDIFSAKRFLCIQPHYDDNDIGAAGILTQLAKNGA
                     EIIYLTVTNDLMGVVDSSLSDDEAAATLKRDQFAAAEIVGVKKQYWLGYPDAGEYGYF
                     DVRRDLLKYIRLLKPDFVFTC"
     gene            complement(367..1362)
                     /locus_tag="PL000_22150"
     CDS             complement(367..1362)
                     /locus_tag="PL000_22150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNC91644.1"
                     /translation="MKIAFAGTGGINKVHARGAQTLGLELVAAVNHRPESMAEFAKEF
                     GIPRQYETIEAMLKDGGVDAVVVSTPNYLHAPQTIAALNAGVHVMVEKPMAMNAHEAE
                     QMCAAADKSGALLMVAHCWRFHPEVQWLKEQSAKLGKIIRTKAYSVHVHWGPSGWFAQ
                     KEFAGGGAMADMGVHALDTTRYLLGDPQPVSVYAKIGTHYGSYDVDDTGVIIIEWDNG
                     TTSYIETGWWQPHSDSPEAGAQLYGLKGFGSVFPTHLEIPNEEAQRIDLIETGFPQQE
                     SHQPQSLYDDQLKYFVGCVEKNQTPIPGGLEGWTNMKVVDAAYESSRTGKAVEIK"
     gene            complement(1376..1864)
                     /locus_tag="PL000_22155"
     CDS             complement(1376..1864)
                     /locus_tag="PL000_22155"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HNC91645.1"
                     /translation="MMNEKEFLEFMDANGFAYERLEHPAVFTCAEADLHHAGVDAVST
                     KNLFLCDKKARRFFLAVTACEKTVKLDELTSQLGVAHLRFGSEENLMHLLGVTRGSVT
                     MMGLANDSEHAVELWIDEEIWRGEQFLCHPLVNTATLILSKPELQRFFALTGHAPKFF
                     KE"
     gene            complement(1861..>2231)
                     /locus_tag="PL000_22160"
     CDS             complement(1861..>2231)
                     /locus_tag="PL000_22160"
                     /inference="COORDINATES: protein motif:HMM:NF036773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HNC91646.1"
                     /translation="GAWNESTQREAYLLNDPIVVYQTKEETGKKPISSLQSLVSCSSP
                     NVIIETVKQAEDGDGIIVRLYESQRKRGQVRVKFRSELEAAWVANLLEENESALSVDQ
                     DSIHLNLKPYQILTLRVKFK"
BASE COUNT          497 a          632 c          518 g          584 t
ORIGIN      
        1 cgcaggtgaa gacgaagtcg ggcttgagca ggcggatgta tttgagcaag tcgcgacgca
       61 cgtcgaagta tccgtattcg cccgcgtcgg gatagccgag ccagtactgc ttttttacac
      121 cgacaatttc cgccgccgca aattggtcac gcttgagtgt cgcggctgct tcgtcatcgg
      181 agagcgaaga atcgacgacg cccatcaggt cattggtaac cgtgaggtaa ataatttccg
      241 cgccgttctt tgcgagctgt gtgagaatgc ccgccgcgcc gatatcattg tcatcgtaat
      301 gcggctgaat gcagagaaag cgctttgcag aaaaaatatc gggagttggg agtatggttt
      361 cgattgtcat ttgatttcca cagcctttcc agttctgctc gactcatacg ccgcatccac
      421 aaccttcatg ttcgtccagc cttccaatcc gccggggatt ggagtttgat tcttttccac
      481 gcatccgacg aaatatttca attggtcatc atacaggctt tgcggctgat gcgattcctg
      541 ctgcgggaaa cctgtttcaa tcaagtcaat tctttgcgcc tcttcatttg ggatttccaa
      601 atgagtgggg aagaccgaac cgaatccctt gagcccataa agttgtgcgc ctgcttcggg
      661 actgtcggag tgcggctgcc accaacctgt ttcaatatag gatgttgtgc cgttgtccca
      721 ctcgatgatg atgacgcctg tatcatccac atcgtaactg ccgtagtgtg tgccaatttt
      781 tgcataaaca gaaacgggct gcggatcgcc aagcaggtag cgcgtggtat cgagcgcatg
      841 cacacccata tctgccattg caccgccgcc tgcaaattct ttctgtgcga accagcccga
      901 gggtccccaa tgcacgtgga cgctgtacgc cttcgtgcgg atgattttcc caagcttcgc
      961 agattgttct ttcaaccact gcacttcagg atggaaccgc caacagtgcg ccaccatcaa
     1021 caacgcgcca gatttatccg ccgcggcgca catctgctcg gcttcatgcg cattcattgc
     1081 catcggcttt tcaaccatca catgcacacc tgcgttcaac gcggcaatgg tttgcggcgc
     1141 atgcaaatag ttgggcgtgc tcaccaccac cgcatccaca cctccatcct tgagcatggc
     1201 ttcgatggtt tcgtattgac gcggaatacc aaattccttt gcgaactccg ccattgattc
     1261 tggtctgtgg ttcacagccg cgacaagttc cagccccagt gtttgtgccc cccgcgcgtg
     1321 gaccttgttg attccgcctg tgcctgcaaa tgcgattttc atcttcaact tcctttcatt
     1381 ccttgaaaaa tttcggggca tgtcctgtca acgcaaagaa ccgctgcaac tctggttttg
     1441 agagaatcag cgtcgctgta ttcaccagcg ggtgacaaag aaattgttcg ccgcgccaga
     1501 tttcttcatc aatccataac tcaacggcgt gttcggaatc attcgccaaa cccatcatcg
     1561 tcactgagcc gcgtgtcacg ccaagcaagt gcatcaggtt ttcttccgag ccgaagcgca
     1621 aatgagccac acccaactgc gatgttaatt catccagctt cacggttttc tcacatgcgg
     1681 tcactgccag aaagaagcgc cgcgcttttt tgtcgcacaa aaataaattc tttgtgctta
     1741 ctgcatccac acctgcatgg tgcaagtctg cttcggcaca ggtgaacaca gcgggatgct
     1801 ccaatcgctc gtacgcaaat ccgttggcgt ccataaattc gagaaattct ttttcattca
     1861 tcatttgaac ttcacccgca gagtaagaat ctgatacggc ttcaaattca agtggattga
     1921 atcttgatcc acgctcaaag cggattcatt ttcctccaac agattcgcca cccaagccgc
     1981 ttccaactcg cttcggaact tgacccgaac ctgtccccgc ttcctctggc tttcatacaa
     2041 gcggacgatg atcccgtctc cgtcttccgc ctgcttcacg gtttcgatga tgacgttggg
     2101 cgaagagcaa gagactagag attggagact agagattggt ttctttcctg tttcttcttt
     2161 cgtttgatac acaacaatcg ggtcattcaa caaatacgct tcgcgttgcg tcgattcatt
     2221 ccatgcaccc a
//
LOCUS       DAOQLC010000311         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_31839_length_2223_cov_21.238930, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000311 DAOQLC010000000
VERSION     DAOQLC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31839_length_2223_cov_21.238930"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1743)
                     /locus_tag="PL000_22165"
     CDS             complement(244..1743)
                     /locus_tag="PL000_22165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC91647.1"
                     /translation="MTTENWKPRELEILQLLAQGLTNSEIGTRLHLSQETVRWYNKQI
                     FGKLGAANRTQAVQQAFEQHLIPDQKRHGSSKPMEISPVRYVANDGVHIAYRVIGTGP
                     VDLLFIHGFISNMELAFEEPEYAKFFEALGKFARVILFDKRGMGLSDRVVGAPSLEDT
                     MGDALCVLDAVESDRTFVMGTSEGGAVSVLLAATHPERVRGLILYASTPKLVQTAGEP
                     EWADDEEAFERSLDRIREEWGGPWAIQSFCPSRAQDKLFCAWWAKVLRSSTSPSEASA
                     VLRVLRDIDIRPLLPQVRTRTLLMHKTHDHIVEVGAGRYIAAQMPNAKYMEFSGGDHF
                     FFVESSRMVAEIVQFVQEDPNLLADTWIGITLYLHLPMVQRKENTIRIEMKEQRAKAI
                     FFGTEEVVAVFDSPNRAIQCALKLRETLKNDALRISLHVGECNVTDGRPSPAVIEIAR
                     HAASLAPPEKILITQTLRDILAGSGVVFDLRQVHIDAKKKDNVSVYAVK"
     gene            1833..2189
                     /locus_tag="PL000_22170"
     CDS             1833..2189
                     /locus_tag="PL000_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91648.1"
                     /translation="MKKTSTTLLVFGIYLVGMGLGLVLMPDFVLSTLGFPPTDEVWTH
                     VVGALALVLAFYYISASRIGLSAFAKMTVPARIGVFILFTVFTLVGWAGPVMVLLGGV
                     DLLGALWTGSALRAEG"
BASE COUNT          495 a          578 c          578 g          572 t
ORIGIN      
        1 aagttcattt tacagagatt tgacaaagag tcaagatgaa aacagctgat tatgcagttt
       61 ccttaatatc tttgtttcag gggcaaaaat gtcctatata tggaaagaag gccttcccgt
      121 gttattttct cctgggttgg ccatcttttg tgtttttata tcgttggttc aaattttttc
      181 gaaatcttta ttgcagcttg tcgatccccc ttcactttgg acactactaa tacatcgtaa
      241 aaactacttt accgcatata ccgaaacatt atcttttttc tttgcgtcga tatggacctg
      301 tcgcaggtca aacaccacac cagaccctgc cagaatatcc cgcagggttt gtgtgatcag
      361 aattttttct ggcggggcca ggcttgcagc atggcgggcg atctcgatta cagcaggcga
      421 gggtctgcca tctgtgacgt tgcattcccc aacatgcagg ctgatgcgca acgcgtcgtt
      481 tttgagtgtt tcgcggagtt tcaaggcaca ctggattgcg cggttgggac tgtcgaatac
      541 agcgacaacc tcctccgtcc caaagaatat cgctttggcg cgctgttcct tcatctcaat
      601 gcggatcgtg ttttcctttc gctgaaccat tggcaaatgg agataaagcg tgatgccgat
      661 ccaggtgtcg gccagtagat tggggtcttc ctgaacgaac tgcacgatct ccgccaccat
      721 gcgcgaactt tccacaaaga aaaaatgatc cccgccggag aattccatat atttggcgtt
      781 gggcatttgt gcggcaatat aacgtcccgc acccacctcc acgatgtggt cgtgcgtttt
      841 gtgcatcaac agtgtgcggg tgcggacttg cggcaggagc gggcgaatat cgatatcacg
      901 taatacacgc agaacggcgc tggcttcaga tggactggtg gaggagcgca acacctttgc
      961 ccaccaggca cagaacaact tgtcctgcgc acgtgacgga caaaagcttt gaatcgccca
     1021 tggcccgccc cattcctcgc ggatacgatc gagcgatctc tcgaacgcct cttcgtcatc
     1081 ggcccattcg ggttcgcctg ctgtctgcac cagtttgggc gtggaggcat agaggatgag
     1141 tccgcgcacg cgctcggggt gagtggctgc cagcaaaacg gagacagcgc ctccttcgga
     1201 tgtacccatc acaaaggtcc gatcagattc gacggcatct aggacacaca atgcgtcgcc
     1261 catggtgtcc tccagtgagg gcgcgccaac cacacggtcc gagaggccca tgccgcgttt
     1321 atcgaacaag atcacacggg caaactttcc caaggcctcg aaaaactttg cgtattcggg
     1381 ttcctcgaag gcgagttcca tattggaaat gaatccgtga atgaacaaca agtccaccgg
     1441 gcccgtgcca atgaccctgt atgcgatgtg gaccccgtca ttcgcaacat aacggacggg
     1501 agatatttcc atcggtttgg atgagccgtg tcttttttga tcggggatga gatgctgctc
     1561 gaaggcctgt tgcacagcct gtgtccgatt cgccgcgcca agcttaccga agatctgctt
     1621 gttgtaccag cgcactgttt cctgagagag atgcaggcgc gtgccgatct cgctgttggt
     1681 gagaccctgt gccaacaatt gcaggatctc aagctcacgg ggtttccagt tttctgtggt
     1741 cattgaagtt ccacccattt tacccacaaa cgtgtggtga caaccaccct gtgcagaacc
     1801 tatactgatg aaaacaaaaa aggagaattg ctatgaaaaa aacttccaca accctgttgg
     1861 tctttggcat ttatttggtt ggcatggggc tgggacttgt actgatgcct gattttgtgt
     1921 tgagcacgct cggttttccg ccaaccgatg aagtatggac ccatgtagtg ggcgcactgg
     1981 cgttggtgct ggcgttctac tacatctctg cctcccgtat cggcttgagc gcatttgcaa
     2041 aaatgaccgt ccctgcccgc atcggtgtct tcattctttt cacggtgttc acattggttg
     2101 gctgggcagg tccggtcatg gttctgcttg gtggcgtaga cttgttgggc gccctatgga
     2161 ctggttcggc attgagagcc gaaggctaac gagacacctg cccatttagc aaaacatccg
     2221 aag
//
LOCUS       DAOQLC010000312         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_33234_length_2170_cov_24.765957, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000312 DAOQLC010000000
VERSION     DAOQLC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33234_length_2170_cov_24.765957"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1750)
                     /locus_tag="PL000_22175"
     CDS             complement(20..1750)
                     /locus_tag="PL000_22175"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91649.1"
                     /translation="MKNHKQLNILDPWAHLGPKRRTLLDNSWAGLFQQHILPELPVES
                     LRHHYHDYNGRPTKELYAMMGVMLLQQMHDCTDQEAVEQFCFNIQWHYALNITSCSDA
                     AAYISHKTLWTMRDHLASDASYAEIFDISLNILAKLLKADMSKQRMDSVHVKSNMRNL
                     GRIGLFSKTIKKFLVNLKRHHRERFDQLNTELTQRYLSKSQGAVFALVKPTESTRTLD
                     QLAADVLLLTERFATEKEVCTMQSFKLLSRLFAEQCVVEEDTTADSGKKAVARPNKDV
                     PSDSLQNPSDPDAGYSSHKGQGYQVQLVENYTTTDERGPSLITQVEVESADQHDANAL
                     LPALAQLEQKAMLPQQMLADSLYGSDSNCETALHEHQVAVISPVMPGNQKKFNLAEFT
                     LDDQGKVLACPQGTTSDTVKKSKSGYNAVFPIAACQDCPSFDQCPVSMGKKGCYYRYT
                     DKDIRLARRRQEEGRPEFREKYRYRAGVEATMSEFDRRTGVKHLRVRGMKAVRFSAFM
                     KAIGLNILRASRLWGGKNNPVTPSCSLFSFLLALGAHFKELVREWFPIKTRMGTDFQP
                     TASLRMDLTV"
BASE COUNT          494 a          542 c          596 g          538 t
ORIGIN      
        1 ttgatgaatc cgtaaaaagt caaaccgtca aatccattcg aagggaagca gtcggttgaa
       61 agtctgtgcc catgcgagtt tttattggga accattccct aacaagctct ttgaaatgtg
      121 ccccgagcgc caaaagaaag gaaaataggc tgcaagaggg tgtcacgggg ttgttttttc
      181 cgccccagag cctactggcc ctcaatatgt tcaaaccgat ggccttcatg aaggcggaaa
      241 accgcactgc tttcatgcct cgaacccgga gatgtttgac acccgtgcgc cggtcgaact
      301 ccgacatggt cgcctcgacg ccggcccggt accggtattt ctccctgaac tcagggcgtc
      361 cctcttcctg acgtcggcgg gccagacgaa tatccttgtc ggtgtaccgg tagtaacagc
      421 cctttttccc catggagacg ggacactggt caaacgaggg gcagtcctgg caggcagcga
      481 tgggaaagac ggcgttgtag cccgatttcg atttcttcac cgtgtctgat gtcgttccct
      541 gcgggcaggc gagaaccttc ccctgatcat caagggtgaa ttcagccagg ttgaactttt
      601 tctgattgcc cggcatcacc ggagagatga ccgccacctg atgctcatgc agagccgttt
      661 cacaattgct gtcactgccg tagagggaat cggccaacat ttgctggggc agcatcgcct
      721 tctgctccag ttgagcgagg gcgggcagga gggcattggc atcatgctga tccgcggatt
      781 ccacctccac ctgcgtgatc agggatggtc ctcgctcatc ggtggtggtg tagttttcca
      841 ccaactgtac ctgataccct tggcctttat gactactgta gccggcatcc ggatcggacg
      901 ggttttgcag tgaatcggat ggtacgtcct tgttcggccg ggccacggct ttcttgccgg
      961 aatcggcggt ggtgtcttcc tcgacgacgc attgttcggc gaacaaccgg ctgagcagct
     1021 tgaagctctg catggtgcag acctctttct cggtggcaaa acgctcggtc aagagcagca
     1081 catccgcagc caactgatca agggtgcggg tggactcggt gggcttgacc agagcaaaca
     1141 cagccccctg cgatttgctc agataccgtt gggtcaactc cgtgttgagt tgatcaaaac
     1201 gctcgcgatg gtgtcgtttc aggttgacca ggaacttctt gatcgttttg gagaacaacc
     1261 cgatgcggcc cagattacgc atgttggatt tgacatgcac ggagtccatg cgctgcttgc
     1321 tcatatcggc cttgagcaat ttggccaaaa tgttcaggga gatgtcaaat atctcggcat
     1381 agctcgcatc cgaggccaag tgatctcgca tggtccagag tgttttgtgg ctgatgtacg
     1441 cagccgcgtc ggagcatgag gtgatattga gggcgtagtg ccactggata ttgaaacaga
     1501 actgctcaac cgcctcctga tcggtacaat catgcatttg ctggaggagc atcaccccca
     1561 tcatggcata cagttccttg gtgggccggc cattgtagtc atgataatgg tggcgcaggg
     1621 actccaccgg aagctccggc aggatgtgct gctgaaaaag acctgcccat gagttgtcca
     1681 gaagggtgcg tcgcttgggt cccaaatggg cccatggatc gaggatgttg agctgtttgt
     1741 ggtttttcac gtgaaacacg gatctgcctc gcattttgtt gatattacga attaaaataa
     1801 caaaatgcga gaaaaatggc aagctgaatc tatagaaatg tagtgtaatt ttagatagtt
     1861 aaacgcaaat actttttgcg agcttatcat ttatttctct tgggaaaacc cccggctttg
     1921 ccgggggact ccaaaagttt gacggttaca gaattgatag caaaaaaata tggtgtggtg
     1981 ctggaagact atttgaggcc agcagtcatt gagaccccgc acccagaaaa tcaccttaat
     2041 ccacgttgat tacccgattc gaatgggtag cagattgacc ctcagttgtc ggtttctttg
     2101 aatgtgctag tctcgggact ccagttgcca aaatcgaaag caggtaaatt cgaactcatt
     2161 ggatttgtct
//
LOCUS       DAOQLC010000313         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_33328_length_2167_cov_17.572443, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000313 DAOQLC010000000
VERSION     DAOQLC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33328_length_2167_cov_17.572443"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PL000_22180"
     CDS             complement(<1..687)
                     /locus_tag="PL000_22180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91650.1"
                     /translation="MKIKLNNGKLVEKYTVADLPAEINWAKSTALKKANHTADTWQGR
                     SPHKRFENIFLGDLAKNIVKKYLLQEIAGVSPFLIEYDQIRTDDFINRDMFDLKIEKD
                     ATSIELEIKSSGEKTISDPSSLNSSRRLIVNQPGPHYHLSDACIQVMFVPANMQFFKN
                     EITASQLSLEKYSEGYIQDFAAASVIAYIMGFADLQMQKNALANLFSVNNSCLRCGVC
                     GRTLGRLRAPS"
     gene            complement(794..>2167)
                     /locus_tag="PL000_22185"
     CDS             complement(794..>2167)
                     /locus_tag="PL000_22185"
                     /inference="COORDINATES: protein motif:HMM:NF039390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91651.1"
                     /translation="LSVNQSGDRMDHPATVHEVNVLTVVASESYKDFVAALQKDISES
                     LSARPRLADEAYFTGKVLKTATGDVEVTPQLAKQIYKYLLKHDYTDDADRITTAYHEA
                     KKDGALAALPPELAPHAEQVFKLIDSVFSESQLPEIGDDRRPKKNPLNANFEKQEFKE
                     LWNRINRKAAYSVDFNSDELVQKAVKTLNDKDAGLRVTPLQYTIQHGEQAAAVTYDGI
                     KGGKAFELKDSETETNRVSIHSAVKYDLIGKLAEGTQLTRRTVTDILKGLNAAVFAQF
                     KTNPESFIAEAIRLINEQKATVIIEHLAYDPVEDKFDLDIFTAGQTKQDFSNAVKTPR
                     HHIYDFVLPDSGSKPESDFAEALEASAEVVVYAKLPRGFLIPTPVGDYNPDWAISFKE
                     GAVKHIYFVAETKGSMSTMDLREIEKTKIKCARRFFDEMNRRHSPENVKYDVVDSFGR
                     LMELVKQ"
BASE COUNT          450 a          557 c          547 g          613 t
ORIGIN      
        1 cgacggcgcg cgcagcctgc ccagcgtcct gccgcagacg ccgcagcgca aacagctgtt
       61 atttacagag aaaagattag ctagagcatt tttttgcatt tgtaagtcag caaaccccat
      121 aatatatgca atcacggagg ctgcagcaaa gtcttgaatg tagccttctg aatatttttc
      181 aaggcttaat tgagacgcag tgatttcgtt cttgaaaaac tgcatatttg ctggcacaaa
      241 catcacttga atacatgcat cagataggtg atagtggggt cctggttgat taacaattag
      301 tctgcgcgaa gagttcagtg aggatgggtc cgaaattgtt ttttcgcctg aggatttaat
      361 ttctagttct attgaagttg cgtccttttc gatttttaaa tcaaacatgt ctctatttat
      421 aaagtcatcg gttctgattt gatcgtattc aatcagaaat ggtgaaactc cggcaatctc
      481 ttgcagcaag tatttcttga caatattttt tgccagatct ccgaggaaaa tattctcaaa
      541 ccgcttatgg ggggatcgcc cttgccaagt atctgccgtg tggtttgctt tctttaaagc
      601 agtgcttttt gcccaattta tttccgcagg cagatcggcg acagtatatt tttctactag
      661 ctttccgttg tttaacttga ttttcatgat ttccctttgt gaaaaacaat cattttctgc
      721 cgaagaaatg cctcctgcat ctgcggaggc aatcaaggct cagatctgtc tgaatttcct
      781 acgatggcgg tatctattgt ttgaccaact ccataagtcg cccaaagcta tctactacgt
      841 catacttcac attttcaggt gagtggcgac gattcatctc atcgaaaaac ctgcgggcgc
      901 atttgatctt agttttctcg atctcacgca gatccatcgt ggacatcgat cctttggttt
      961 ccgcgacgaa gtaaatgtgc ttcactgcgc cttccttgaa cgagatggcc cagtccgggt
     1021 tgtagtcgcc taccggtgtc gggatcaaga aaccgcgcgg caacttggca tacacaacga
     1081 cttcagcgct ggcctccaac gcctccgcaa aatcgctctc cggcttcgat ccggaatccg
     1141 gcagcacgaa gtcgtaaatg tggtgcctgg gagtcttgac tgcgttgctg aagtcctgct
     1201 tggtctgccc ggctgtgaag atgtccagat cgaacttgtc ttccaccggg tcataggcca
     1261 agtgctcgat gatgaccgtg gccttctgct cgttgatcag gcgaatcgcc tcggcgatga
     1321 agctctccgg gttggtcttg aactgcgcga atacggccgc gttgagtccc ttcagaatgt
     1381 cggtcaccgt acgccgtgtc agctgggtgc cttctgcaag tttgccgatt aggtcatact
     1441 tgacagctga atggattgaa acgcggtttg tttccgtctc agagtccttc aattcgaaag
     1501 ctttgccgcc cttgatccca tcgtaggtca cagcggctgc ctgttcaccg tgctggatgg
     1561 tgtactgcaa tggggtcaca cgaagcccag catctttgtc gttgagtgtc ttgaccgctt
     1621 tctgcaccag ctcgtcggag ttgaagtcga cgctgtacgc cgctttacgg ttgatccgat
     1681 tccaaagctc cttgaactcc tgcttctcaa agttggcgtt cagcgggttc ttcttgggcc
     1741 tgcggtcgtc accaatttcg ggcaactggc tctcgctgaa gacactgtca atcagtttga
     1801 acacctgctc ggcatgtggt gccaactcgg ggggcagcgc agccagcgcg ccgtctttct
     1861 tggcctcgtg gtacgcggtg gtgatgcgat ctgcgtcgtc ggtgtagtcg tgcttgagca
     1921 ggtacttgta gatctgcttg gcgagttgcg gtgtgacctc gacatcacca gtcgctgtct
     1981 tcagaacttt gccggtgaag taggcctcat ccgccaagcg cggacgcgcc gacagcgact
     2041 cgctgatgtc cttctgcagc gctgccacga agtccttgta gctctcgctg gccaccacag
     2101 tcagcacgtt cacttcgtgt accgtggccg ggtgatccat cctgtcaccg ctttggttga
     2161 cggacag
//
LOCUS       DAOQLC010000314         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_33489_length_2161_cov_26.563628, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000314 DAOQLC010000000
VERSION     DAOQLC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33489_length_2161_cov_26.563628"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..2019
                     /gene="istA"
                     /locus_tag="PL000_22190"
     CDS             529..2019
                     /gene="istA"
                     /locus_tag="PL000_22190"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HNC91652.1"
                     /translation="MGVSKEVESEILRLFYAEKWKRGTIARQLQIHHGVVDRVLTRNG
                     ISVERVRIRRSLIDPYLPYIRSVLQKYPRLTGSRLYAMVKERGYPGGIDHFRDMLARH
                     RPKPAAEAYLRLSTIKGEEAQVDWACFGKVKIGNAERQLYAFVIVLSWSRQVFLKFYT
                     GMSTANFLQGHVDAFCSWGGVPRQVKYDNLKSAVLERSGDAIRFNPRLLDFAAYYRYA
                     PKPVAIARGNQKGRVERAIQYIRHSFFAARKWDGLEDLNNQAIVWCREVAGTRPCSED
                     RTMTVAEAFKQEQGSLMPLPDNPYAVFESTVVSVGKAPYVRYDMNDYSVPHEYVQKQL
                     TVHADSDWVRILDGLKEVAKHKRCFEKRRQIETTEHISRLVEEKKKAKKGSGMNRLFC
                     SAPTAREMFRMAAERGYSIGSLTSKLLRLLDLYGPAEVEESIKEVVTSGACHSADVAN
                     VLERRRRQKGLLSPFAIDLPDDKRVTDLVVLPHSLATYDNLSKEEQ"
BASE COUNT          553 a          458 c          681 g          469 t
ORIGIN      
        1 aacttgaacc cctggattca tctattggac gatgaaggag agagtttcct acaagaattg
       61 agtcctgtcc aggccttttt gctcattgag tggacgatga gcaagattcc tttcgactgt
      121 tcaactgcgc ccgatgccgg gaacaggtaa ggctttgcag gagctgtgac cggggtaacc
      181 gatattgcag ccaggattgc gcgaaattaa accgccgaca gggacaggct gaggccgggc
      241 ggcgttacca gcgcagcttt aggggtgcgc taaatcacag ccgccggcag gcagaataca
      301 agaggcggtg caaagaagaa gtgacgcatc atggttccct tctggcttgt caggatgaca
      361 ttgtttgtcc agagaccgag gaagtggtct ctacggaaat cacaaaattc gagccggctg
      421 aagatgcagt gttaattcgc tgcagcggtt gcggccgtct ttgtgggccg ttcgcacgcc
      481 aagacttcat cgggcgatgt cgcctgggaa gaacaacagg aggtaacagt gggcgtttca
      541 aaagaagtag aatcagagat cctgcgcttg ttttacgcag agaagtggaa aagaggcacc
      601 atcgccaggc agctacagat tcatcacggt gtagttgatc gggtattaac cagaaatgga
      661 attagcgttg agcgggtcag gatcaggcgt tcgttgattg acccgtattt gccgtacatc
      721 cgttctgtgc tgcagaagta tccgagactg acgggatcgc ggctgtatgc aatggtgaaa
      781 gagagagggt atcctggggg catcgaccac tttcgagaca tgctagccag gcatcgacca
      841 aaaccggcag ctgaagcgta tctcaggctg agtacgatta agggagagga ggctcaggtt
      901 gactgggcgt gcttcgggaa agtgaaaatc ggtaatgccg agcggcagct gtacgctttc
      961 gtcattgtgt tgtcctggtc acggcaggtg tttctgaagt tctacacagg catgtccacg
     1021 gcgaactttc tacaggggca cgtagacgcc ttttgcagtt ggggaggtgt gccgaggcag
     1081 gtcaagtacg acaatttgaa atcggccgtt ttggagagga gtggcgatgc catccgattc
     1141 aatccgcggc tgctggactt tgcagcgtat taccgatatg ccccgaagcc agtagctatc
     1201 gccaggggca atcaaaaggg gcgagtcgag agggcgatac agtacatcag gcattcgttc
     1261 tttgctgcgc ggaagtggga tggtctggag gacctgaaca atcaggcgat agtctggtgc
     1321 cgagaagtag caggcaccag accgtgttca gaggaccgaa cgatgactgt agcagaggca
     1381 tttaagcaag agcaagggag tttgatgccg ttgccggaca atccgtatgc agtatttgag
     1441 agcacggttg tatcggttgg caaggcaccg tatgtcaggt atgacatgaa cgattattca
     1501 gtacctcatg aatacgtgca gaagcagctc acggtgcatg cggacagcga ttgggtgcgt
     1561 atcttggacg gattgaagga ggtagcgaag cacaagcgat gctttgaaaa gaggcgtcag
     1621 attgagacta ccgagcacat ttcgaggctg gtcgaagaga agaaaaaggc aaagaagggc
     1681 agcggcatga atcgactatt ttgctcggca ccaactgcaa gagagatgtt ccgtatggct
     1741 gcggagcggg gttattcaat cggttcgctg acgagcaaat tgctgagact gttggactta
     1801 tatggtccgg cagaagtgga ggaatcgatc aaggaagtgg tgacttctgg agcttgccac
     1861 tctgcagatg ttgccaacgt acttgagagg aggaggaggc aaaaggggtt gctatcgcca
     1921 tttgcgattg atttaccgga cgacaaaagg gttactgatc tcgttgtttt gccgcactcg
     1981 ctggctactt acgacaatct ttccaaggag gaacaatgac gacagatttg aaagaacagg
     2041 cgagcaagct gggcttcaag gccatggcgg ccagatggaa tgaatatgcc aatcaatcgt
     2101 gggtcaaaga actgttagag gctgaggcgg cggagaagaa tcggcgaagc ttagagcggc
     2161 g
//
LOCUS       DAOQLC010000315         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_33619_length_2157_cov_25.445766, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000315 DAOQLC010000000
VERSION     DAOQLC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33619_length_2157_cov_25.445766"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PL000_22195"
     CDS             <1..347
                     /locus_tag="PL000_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91653.1"
                     /translation="KAVMERLGFHEQGRYFIHPETTFFIEFPDGPLSVGEEPVKEISE
                     FELSTGTLRVLSPTDCVKDRLCAFYFWNDLQGLEQATLVTKSQKVDLKEIKRWSKVEG
                     KVKEFKAFTEKL"
     gene            429..2090
                     /locus_tag="PL000_22200"
     CDS             429..2090
                     /locus_tag="PL000_22200"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HNC91654.1"
                     /translation="MKPNIFMSYSRRETGFVDDLTYRLEKAGFNVWLDFRRLIPGTPW
                     AGQIDKGLRESDVVLLVVSKDSIASQFVELEWRHVLEYEHKRIILAIFEAVKLPPELK
                     KYEWVDFRGSYEAGLKELIGQLNTPMQEMQPAPETGFKVPLIVWVTVIFAFFLALSSL
                     YSFWTILIPLILLPLPLQVLKRNYNFSRIQSILVFQPVSLFLVVLLMFETGVFQNEAI
                     LGLSDFYYSLSPFFRFLYDAMQWMFFLSIFLPFFLLRTAGMQRWGKPEANMPKFANPY
                     KPNNPKPKPITFFIDHVEQDSVMAKDISKGLTRYGHIPAADLKSAEAVFALVSRFKTD
                     TEADPEKQTVFPVMVQTAQPSEKLSHVQWVDFRKGLRNIDAVAQLLPDPAKMLTALGV
                     RPTSGTQTVMPGIISAFVNFLLGMVFLDIGSLAAYVIELWAFDGGAIMLPGILYFGMY
                     IIAMFLIFGVIYLVNTSLTARRGWLASINGLIFSQALLFVLFYWQAAQVDNIDALYAQ
                     SGVTDPGGYFIMVPFLVYIAGGFLLSAAALFRFSDIRRWFPAKTT"
BASE COUNT          521 a          509 c          522 g          605 t
ORIGIN      
        1 tcaaagccgt gatggaaagg cttggcttcc acgagcaggg acgttacttc atccatcccg
       61 aaacaacctt cttcatcgag ttccccgatg gaccactatc tgttggagaa gaacctgtga
      121 aagagatcag cgaattcgag ctttccacag ggacattgcg agtgctctcc cctacggact
      181 gtgtcaaaga caggttgtgt gcgttctact tctggaatga cttgcaggga ttggagcagg
      241 caaccttggt gacaaaaagc caaaaagtgg acttgaaaga aatcaaacgt tggtcgaagg
      301 tagagggaaa agtaaaagaa tttaaagcat ttacagaaaa gctttgagct tccccccacc
      361 acccgattgg attgcagtgt ggtttaatat ttgaacgcag tacattcagc ctgccaaagg
      421 atcattaaat gaaaccaaat atctttatga gttattcacg ccgcgaaact ggttttgtag
      481 atgacctgac ctataggttg gaaaaggcag ggtttaatgt ttggctagat ttccgccgat
      541 taattccagg cacaccttgg gcagggcaga tcgacaaggg attaagggaa tcagatgtgg
      601 tcttattggt ggtttccaaa gattccatcg cgtcgcagtt cgtggaattg gaatggcgcc
      661 atgtattgga gtatgagcac aagcgcatca ttctcgctat tttcgaagcg gtaaaacttc
      721 cgcctgaatt aaaaaaatac gaatgggtgg attttcgcgg cagttacgag gcggggctta
      781 aggaactcat cggtcaatta aataccccaa tgcaggaaat gcaacctgca cctgaaaccg
      841 gcttcaaagt gcccttgatc gtttgggtga cggtgatctt tgcttttttt ctcgcccttt
      901 cttcgctata ttcgttttgg acgatcttga tcccgctgat cttgctcccg cttcccttgc
      961 aggtactcaa acgcaactac aatttctcgc ggatccagag catcctggtg tttcagcctg
     1021 tttcactatt tttggtcgtc ctgctaatgt tcgaaacggg cgtattccaa aatgaggcaa
     1081 tattggggtt gagcgatttt tactattcct tatccccatt tttcaggttc ttgtacgatg
     1141 cgatgcaatg gatgtttttc ctgtccatat tcctgccgtt cttcctgttg cgcactgcag
     1201 ggatgcaacg ctggggcaaa cccgaagcca atatgccaaa gtttgccaac ccgtataaac
     1261 caaacaatcc caagcccaag ccaattactt tctttatcga tcatgttgag caggattctg
     1321 ttatggcgaa ggatatctca aagggattga ccagatatgg gcacatcccc gctgcggatc
     1381 ttaaatccgc tgaagcagtc ttcgcgttgg tctcacgctt caaaacggac acggaagctg
     1441 atccagaaaa acagaccgtt ttccccgtta tggttcagac cgctcaacct tcagagaaac
     1501 tctctcacgt gcagtgggtc gattttcgta aggggttgcg caatatagat gcagttgctc
     1561 aattgctgcc cgaccccgca aagatgctga ctgcattagg cgtgcgcccc accagcggta
     1621 cacagaccgt catgccgggc atcattagcg ccttcgtaaa ttttttactg ggcatggttt
     1681 ttctcgatat tggctcatta gccgcctatg tgatcgagct ctgggcattt gatggcggcg
     1741 ccattatgtt gccgggtatt ctatattttg gcatgtatat catcgccatg tttctcatct
     1801 ttggggtgat ctacctggtg aatacctcgt tgaccgcgcg ccgcggctgg ctggcttcga
     1861 taaacggttt gattttttca caagcgttgt tatttgtatt gttctactgg caggcagcac
     1921 aagttgataa tatagacgca ctttatgccc aaagcggtgt gacggatccg ggcgggtact
     1981 tcattatggt ccccttcctc gtgtatatcg caggcggttt ccttctaagt gcagcagccc
     2041 tgttccgctt cagtgacatt cgacgctggt tcccagccaa gacaacgtga gatggcagca
     2101 tattgttatg ctggggaagt gctataaagc aaattcaact tgcgttctga ttctccc
//
LOCUS       DAOQLC010000316         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_34530_length_2125_cov_24.142512, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000316 DAOQLC010000000
VERSION     DAOQLC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34530_length_2125_cov_24.142512"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PL000_22205"
     CDS             <1..181
                     /locus_tag="PL000_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91655.1"
                     /translation="PIQIIEKNTFKIICDLKVIQMDTPRTSLKVTLEEGVLRKLLRWE
                     TANFTDRNIIHLNSP"
     gene            complement(475..1491)
                     /locus_tag="PL000_22210"
     CDS             complement(475..1491)
                     /locus_tag="PL000_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91656.1"
                     /translation="MNASTRINLSRLVNAFIDAGIIENVVEAPVPVGDVSWDDFDRIS
                     QANLLASAVYTAYAAVVNTLWREERGDSRPVFDASDDYPYEEDNSSSSFGEPLTLEQA
                     IHNASVVRTMVRSYASDNKWLNGWVNLTLVKDEYGNPVLDKNGKTVFREELVKSFSAP
                     ITQFFDTTDVDEVWRDKVALLRAHKINVPFDGEGIDAVKTDFIEWLAVQTLEDDPRDR
                     LTRFAEKVGDKKWDALKSKALSGKPVTKEDLRHWVLMSYSPKFQADAQLIRESVYYKG
                     YKLDCEIEREEVERAAAELDAMQAQLTAVRTARLAKLAEPSVKVPESVLPKRLNSSGR
                     IVSF"
BASE COUNT          653 a          543 c          421 g          508 t
ORIGIN      
        1 accaattcag atcatcgaaa agaacacctt caagatcatc tgtgatctga aggttatcca
       61 gatggataca cctaggacaa gcttgaaagt taccctcgag gaaggtgtgc tccgtaagct
      121 gctccgctgg gaaacagcaa atttcacaga caggaatatc attcatctca actctcctta
      181 atatgatgac ctgcaaacaa aacgcttggt aaagcctttc gatccacgcc ctttcgcgta
      241 gagtacttcg tcataaccga aataagtgcc ccagacgtcg aacatagggc ggcactttcg
      301 tgccacagtt acgccgtaaa caaaggactt cttaggcaca atctccaagc ctgctttctt
      361 tgcttgtcga aagttcatca caactctcct cactaagcga tgcactcacc atgaatgcac
      421 ctccaactga ggaaacaaaa acgagggagc cgaagccccc tcgcaaacta gaaattagaa
      481 cgaaacgatc cttccagagc tattgagacg cttaggaaga acagactcag gaaccttcac
      541 agaaggttca gccaacttag ccaaccgagc cgttctaaca gcggtaagtt gagcctgcat
      601 tgcatccaac tccgctgcag cacgctcgac ctcctcacgc tcgatctcgc aatcgagctt
      661 ataacccttg taatacaccg actcacgaat gagttgtgca tcagcctgga acttcggcga
      721 ataagacatg agtacccagt gacgaagatc ttccttcgtc acaggcttgc ccgacaacgc
      781 cttgctcttc aaagcatccc acttcttatc gccgaccttc tcagcaaacc gagtcaaacg
      841 atcacgagga tcatcctcga gcgtctgcac agcaagccat tcgatgaagt cagtcttgac
      901 agcgtcgata ccctcgccgt caaacggcac gttgatcttg tgcgcacgaa gcaaagcaac
      961 cttatcgcgc cacacctcat cgacatccgt cgtatcaaag aactgagtga tcggtgcaga
     1021 aaaggacttg accaactcct cacggaacac ggtcttacca ttcttatcga gaacaggatt
     1081 accgtattca tccttgacaa gcgtaaggtt gacccagcca ttcaaccact tgttgtccga
     1141 tgcataactg cgcaccatag tgcgtacgac actagcatta tggatcgctt gctccaaagt
     1201 aagcggttca ccaaacgaag aggacgaatt gtcctcttca taaggataat cgtcagaagc
     1261 atcaaacacc gggcggctat caccacgctc ttctctccac agcgtattca caactgctgc
     1321 gtacgccgta taaactgctg acgcaagaag gttggcttgg gaaatacgat caaagtcatc
     1381 ccacgaaaca tcgccgacgg gaacaggagc ttcaaccaca ttctcgatga taccggcgtc
     1441 gatgaaagca ttaacaaggc gagacaagtt gatacgagtg cttgcgttca ttgcaatctc
     1501 ctaaaaagga atgaaaacgt cgcccataga cgacatttga gatgcgaaat gcacctcaat
     1561 ctgaacactt tttcgaatgt tcaggttgag aaacacctgt aaaaacatac agcatataag
     1621 caattcctgt tataccattt gcttaattgc caatagcata acgaccctca atgcctattt
     1681 tttaggcaaa aaggtccaaa gttttggaac aaacaggagt tttggaacag ttttggaaca
     1741 gttttggaac aacgcaagtg cttgtttcta ctaaagaaag tggcattttg ttccaaagtt
     1801 ccaaaacttt cttctatttg tatacgaaca gtgaaatagt ttgatatttc gagaatcgtc
     1861 gaaacgtcaa taactacaca caaatacgta tacgtataca aactcaacgc gaaaaatcgg
     1921 agttttggaa caaaatgcct gtttcctgtt taaaatcata cagttaaggt gttccaaaac
     1981 tgttccaaaa ctgttccaaa actcaagttt tggaacaaaa tgaaaaattc ttgttctgtg
     2041 acaagcactt acgtgccttg agttttggaa cagtatcgaa attgcataaa gcaattctgt
     2101 tcacaccatt gtgaacatgt acgag
//
LOCUS       DAOQLC010000317         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_35929_length_2081_cov_21.853899, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000317 DAOQLC010000000
VERSION     DAOQLC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35929_length_2081_cov_21.853899"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PL000_22215"
     CDS             complement(<1..982)
                     /locus_tag="PL000_22215"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HNC91657.1"
                     /translation="MLNNPHRRYNALTGEWVLVSPHRAKRPWLGQVEKLSPETLPAYD
                     PTCYLCPRNERAGGVFNSDYAGTYVFNNDFASLLPDAPEEDASASSHPLLVSAPEQGQ
                     CLVVCFSPRHDLTLPELELPVIENVLNTWTNESARLAELDYIKYVQVFENKGAMMGCS
                     NPHPHSQLWSQSQLPNEIVKELATQTEYFKKNNSPLLVDYVKEEHKRKDRILFSNDHF
                     TALVPFWAVWPFEVLVSAHRASASLKELSAVEISALAEVFKQVTIRYDNLFEISFPYS
                     MGFHQAPADGQPHPEWTLHAHFYPPLLRSATVRKFMVGYEMLAMPQRDITP"
     gene            complement(985..>2081)
                     /locus_tag="PL000_22220"
     CDS             complement(985..>2081)
                     /locus_tag="PL000_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91658.1"
                     /translation="ESDRYLFKFADGATFTILDKWTSKSTTVWGDHQTSRYGIFDVTQ
                     PIVQKVLRERAKEFIRRYKPDLIKFDFGYELPSLDVAAPTDMNWAGERLLQKGLEVVV
                     GAMKEENPNLVIMYYGLSPLLIEYYDLHSPDDLVYCIGDYDLETNRRVFFSSLCGELG
                     MPTYSSSGYEWESAPDIWFDTIASGTLGSLHCFDGDESGGLPTDEVIAKFNGLAAIAR
                     KQVIFQTYPVHTKWQGGLRAGFSPSWERIENDKTVLLALRTHHLDGKPAPKNYKDILH
                     TDVTLVVASLTDDGISESLRIGIVPFGDGSCTISHTSLHNSTTIIEHYFSGHATKTQT
                     ACSNSQIEIKLQQTRNHEILEWIEIIFEKE"
BASE COUNT          461 a          455 c          634 g          531 t
ORIGIN      
        1 cgggagtgat gtctcgttgc ggcatggcga gcatttcgta gccgaccata aattttcgga
       61 ccgtggccga gcgcagtagc ggcggataaa aatgcgcatg cagagtccac tcgggatgtg
      121 gctgcccatc agcaggagct tgatggaatc ccattgaata ggggaatgaa atctcgaaca
      181 aattgtcgta tcttatcgtg acctgtttga agacttcagc caatgcagag atttccaccg
      241 ctgacagttc cttcaacgaa gcggatgcgc gatgcgcgct cacaagcacc tcgaacggcc
      301 acaccgccca aaaaggaacc aacgctgtga aatggtcgtt ggagaatagg atgcggtctt
      361 tgcgtttgtg ttcttctttc acataatcca ccagcaatgg actgttgttc ttcttgaagt
      421 attccgtttg cgtggcaagt tctttgacaa tttcattcgg cagttgtgat tgtgaccaaa
      481 gctgactgtg cgggtgcgga ttggaacatc ccatcattgc gcctttgttt tcaaagacct
      541 gcacgtattt gatgtaatcc aattcggcga gtcttgcaga ttcatttgtc caggtgttga
      601 ggacgttttc gatgacaggc aattcgagtt cgggcaatgt caggtcgtgg cggggggaga
      661 agcaaactac cagacattga ccctgttcgg gagcagagac aagcaacggg tgggatgagg
      721 cggaagcgtc ttcttcaggg gcatctggca gcagggaagc aaagtcgttg ttgaagacgt
      781 aggtgccagc ataatccgaa ttgaaaacgc cgccagcccg ttcgttgcgc ggacagagat
      841 agcaggtggg gtcgtaggcg gggagggttt cgggggaaag tttttcgacc tgcccaagcc
      901 agggacgttt ggcacggtgc ggcgagacga gcacccattc gcccgtgagg gcgttatagc
      961 ggcggtgagg attgtttaac atggttattc cttctcaaat atgatttcga tccattcaag
     1021 gatttcgtgg tttcgggttt gttggagctt gatttcgatc tggctgtttg aacaggcagt
     1081 ttgcgttttc gttgcgtgtc cgctgaaata atgctcgatg atggtcgtgc tgttgtgaag
     1141 tgaagtatgg ctgattgtac aactgccgtc gccaaatggg acgatgccga tgcggagtga
     1201 ttctgaaatg ccatcgtcgg ttagggatgc gacgactagc gtgacatcgg tgtgaaggat
     1261 gtctttgtaa tttttcggcg cgggctttcc atcgaggtga tgggtgcgaa gtgcaaggag
     1321 gacggtcttg tcgttttcga tgcgttccca tgagggagaa aagccagcgc gcagaccacc
     1381 ctgccatttt gtatggacgg gataggtttg aaaaataact tgcttgcgag cgatggcggc
     1441 aagcccattg aattttgcaa tgacttcgtc tgtgggcaag ccgccacttt catctccgtc
     1501 gaagcagtgc agggagccga gcgtgcctga ggcgatggtg tcgaaccaaa tatcgggcgc
     1561 ggattcccac tcgtagcctg aggaactgta agtgggcatt cccaactcgc cgcacaaact
     1621 gctgaaaaag acgcggcgat tggtttcaag gtcgtagtca ccgatgcaat agacgaggtc
     1681 atcggggctg tgcaaatcgt agtattcaat gagtagcggc gagagaccgt agtacatgat
     1741 gacgaggttt gggttttctt ctttcatcgc gccgaccaca acttccaatc ccttttgcag
     1801 aaggcgttcg cctgcccaat tcatgtcggt tggggcggcg acatcgagcg aggggagttc
     1861 gtagccgaag tcaaatttga tgaggtcagg tttgtagcgg cggataaact ctttggcgcg
     1921 ctcgcgcaat actttttgga cgatgggttg tgtgacatcg aagatgccgt agcgggaggt
     1981 ttgatggtcg ccccacacgg ttgtgctttt gctggtccat ttatcgagga tggtgaatgt
     2041 tgccccatct gcgaatttga aaagatagcg gtctgattcg g
//
LOCUS       DAOQLC010000318         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_36232_length_2072_cov_28.381755, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000318 DAOQLC010000000
VERSION     DAOQLC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36232_length_2072_cov_28.381755"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1963)
                     /gene="ppk1"
                     /locus_tag="PL000_22225"
     CDS             complement(<1..1963)
                     /gene="ppk1"
                     /locus_tag="PL000_22225"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNC91659.1"
                     /translation="MEKEFLEAQSELNDPSLFLNRELSLLEFQRRLLDEAKDERNPLL
                     ERVKFLAILGSNLDEFFMVRVSGVRRQVEAGVTELSIDGMTPRDQLAAMRKFASELYK
                     EAHTCLTKKLMPKLDKAGIHVMEYEKLTKTQKEKVDAYFREMIHPVLTPLALDPGHPF
                     PHISNLSLSLAVVIHDPKGNERFARLKVPDTLPRLVPIKRSSGGERKDGTIPRHHYFV
                     WLEQVIAANIEMLFPGMHVVSAHPFRTIRDADVEIQEIEADDLLETMQQSIRRRKFGS
                     VVQITVNEAMPEHIRELLVDNLEAKPNDIAVQSNPLGLASLWQLYKSVEKHELKYVPY
                     TPRIVKALKNLESTEDFFAAIRQQNILLHHPYISFSSVVDFLNAAARDPHVLAIKQTL
                     YRVGNNSPIVEALLEASERGKQVAVLVELKARFDEESNISWARKLEQAGVHVVYGLMG
                     LKTHCKVGLVVRQEGEGIRRYVHLGTGNYNAVTANLYEDLGMFTADETIGTDTTDLFN
                     FLTGYSAQQTYRKLLVAPVCLREKFEALILREIEHAKNGRKARLIFKANAIVDPRIIQ
                     LLYRASQAGVKVDLIIRGVCCLRPGVKGISENIRVISVVGRYLEHSRIYYFQNDGAEE
                     IYLGSADLMSRNLNRRVEVAFPIEDAEHIR"
BASE COUNT          465 a          536 c          548 g          523 t
ORIGIN      
        1 ggcggatgtg ttcagcatct tcgatgggga aggccacttc aacacggcga ttcaaattgc
       61 gcgacatcaa gtcggcgctg ccaaggtaga tctcttcggc gccgtcgttt tgaaaataat
      121 aaatgcggct gtgttcaaga taacgtccga ccacactgat gacacggata ttctcgctga
      181 ttcctttaac gccggggcgc aggcagcaca ccccacggat gatcagatcc actttgactc
      241 ccgcctgtga ggcgcgatac aacagttgaa tgatgcgcgg gtctacgatg gcattggcct
      301 tgaaaatgag ccgtgccttt ctgccgttct ttgcatgttc gatctcgcgc aggatcaggg
      361 cttcgaattt ttcgcgcagg cataccggtg caaccagcaa tttgcgatac gtttgctggg
      421 cagagtaacc cgtgagaaag ttgaacaagt cggtggtgtc ggtgccgatg gtttcatcgg
      481 cagtaaacat gccgaggtct tcatataggt tggctgtgac cgcattgtag tttccggtgc
      541 cgaggtgaac atatctgcga atgccttcac cttcctgccg taccacaagg ccaactttgc
      601 aatgtgtctt cagccccatc aaaccgtaca ccacatgtac accggcctgt tccagtttgc
      661 gtgcccagct aatgttgctt tcttcatcaa agcgggcttt gagctccacc aacacagcca
      721 cctgtttgcc gcgttctgag gcttcgagca gggcttccac aatgggcgag ttatttccaa
      781 cacgatacaa ggtctgtttg atggccaaca catgcggatc acgagccgcc gcgtttagaa
      841 aatcgaccac cgaggaaaac gaaatatagg ggtgatgcag caggatattc tgctggcgga
      901 tggcggcaaa gaaatcttca gtggactcaa gatttttcag cgctttgacg atcctggggg
      961 tgtagggcac gtatttcagt tcatgctttt ccacgctctt gtaaagctgc caaagggaag
     1021 ccagccccag cggattcgat tgcacagcga tgtcgtttgg tttggcttcg aggttatcca
     1081 ccaacagttc gcggatgtgc tccggcatcg cttcattaac tgtgatctgc accaccgagc
     1141 cgaattttct gcggcggatg ctttgctgca tggtttcgag caggtcatcg gcctcgatct
     1201 cctggatctc cacatctgca tcgcgaatgg tgcggaatgg atgcgcagaa accacatgca
     1261 tgcccgggaa aagcatttcg atatttgcgg cgatgacctg ttccagccag acaaaatagt
     1321 ggtggcgtgg aatggtgcca tccttgcgct cgcctcccga cgaccgtttg atgggtacca
     1381 agcgaggaag tgtatctggc actttgagac gcgcaaagcg ttcattccct ttcgggtcgt
     1441 ggatgacaac agccagactc aaactcaggt ttgaaatatg cggaaacggg tggccgggat
     1501 caagcgccag cggagtcaaa acagggtgga tcatttcgcg gaaatacgca tccacctttt
     1561 ccttttgggt ctttgttaat ttttcgtatt ccatcacatg aatgcccgct ttatcgagtt
     1621 tgggcatcag ctttttggtc aggcaggtgt gggcttcctt gtagagttca ctggcaaatt
     1681 tacgcattgc ggcaagctgg tcgcggggtg tcatcccatc aattgaaagc tcggtgaccc
     1741 ccgcctccac ctgcctgcgg acacccgaaa cgcgcaccat gaaaaattca tcaaggtttg
     1801 aaccgaggat ggcgagaaat ttcacccgct ccaacaatgg gttgcgctca tctttggcct
     1861 catccagcag gcgtctttga aactccagca ggctgagttc gcggttcaag aacagtgacg
     1921 ggtcattcaa ctccgactgg gcttcgagaa attctttttc catgctttgc ttatgagttt
     1981 ttgccaattt gcaccaactg tgaaggggat agcagccagt tcaggctggc acactttcca
     2041 tattctaatg catccgttgt aagcaggcat ac
//
LOCUS       DAOQLC010000319         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_36604_length_2060_cov_23.670823, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000319 DAOQLC010000000
VERSION     DAOQLC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36604_length_2060_cov_23.670823"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /gene="aroA"
                     /locus_tag="PL000_22230"
     CDS             <1..1032
                     /gene="aroA"
                     /locus_tag="PL000_22230"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNC91660.1"
                     /translation="ELFIGNAGTAARFLSAFLTLGNGEYILDGEPRMRERPIGDLVEA
                     LAQLGCDIQSLEKVESGKSICPPIKINASGLKGGKTKIAGDISSQFLSALLMTAPYAR
                     SPIEVELTTDLNSKPYVDMTLSIMKDFGVEVERDGYQSFKVLPSNYQLPITNYQIETD
                     ASAASYFFAAPAICGGTVKVENISHKSVQGDVNFLDVLAQMGCEVKEADNSILVTRNS
                     SLHGVDVDMRDIPDTAQTLAAVAPFADSPTRIRGIASARVKETDRVHATCTELARLGV
                     QVEEHEDGMTIHPTFNLQPANIQTYNDHRMAMAFSLIGLKFDGVTIENPSCVSKTFPN
                     FFEVLETLR"
     gene            1029..1862
                     /gene="aroE"
                     /locus_tag="PL000_22235"
     CDS             1029..1862
                     /gene="aroE"
                     /locus_tag="PL000_22235"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNC91661.1"
                     /translation="MIRLGLIGYPLGHSLSPKIHAAALKACNLQGDYSLFPIHPEDKP
                     ALQALLARVRAGEITGLNVTIPHKQNVIEFMDELTPTAQVIGAVNMIYMRGDKLIGDN
                     TDATGFLSDLKRVSNSSFSIPHSALVLGAGGSARAVVYALLNDGWNVTITARRIEQAQ
                     QLASSFTNYQLQVADFSSFSIHHSSLLVNTTPLGMTPNTETSPLPENTVLSKNTFVYD
                     LVYNPRETKLVRDARAQGLNASTGLGMLIEQAALAFEKWTGHNPPRDVLFESVGATRP
                     D"
     gene            1913..>2060
                     /locus_tag="PL000_22240"
     CDS             1913..>2060
                     /locus_tag="PL000_22240"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC91662.1"
                     /translation="MLRFLTAGESHGPSLTTILDGLPAGLSLSPDIINKELARRQQGY
                     GSGGR"
BASE COUNT          523 a          578 c          449 g          510 t
ORIGIN      
        1 gaactcttca tcggcaatgc gggcaccgcc gcgagatttt tgtcagcatt tctcacgctc
       61 ggaaacggcg aatatattct ggatggcgag ccgcgtatgc gtgaacgtcc gattggggat
      121 ttggtggagg cgttggctca acttggttgc gatattcagt cactggagaa agtggaaagt
      181 ggaaagtcaa tctgcccacc aataaagatc aatgcttcgg gattaaaagg tggaaaaaca
      241 aaaattgcag gtgatatttc ttctcagttt ttatctgctt tgttgatgac cgctccctac
      301 gccagatctc ccatcgaagt ggaactcacc accgacctca actccaaacc ttacgtggac
      361 atgactctct ccatcatgaa agatttcggg gtggaagtgg aacgggatgg ttatcagtca
      421 ttcaaggttt taccctccaa ttaccaatta ccaattacca attaccaaat cgaaaccgat
      481 gcctccgccg cctcctactt ctttgccgcg cctgccatct gtggtgggac ggtcaaagtg
      541 gagaacatct cgcacaagtc tgtgcagggt gatgtgaatt tcttagacgt acttgctcag
      601 atgggttgtg aagttaaaga agccgataac tcgatactcg ttactcggaa ctcatccctc
      661 catggcgttg acgtagacat gcgcgacatc ccagacacag cccaaaccct tgccgccgtt
      721 gctccttttg cggattctcc cactcgaatt cgcggcatcg cctcggctcg tgtcaaggaa
      781 acagaccgtg tccacgcaac ctgtaccgaa cttgcccgtt taggtgtgca agtcgaagag
      841 catgaagacg gtatgaccat ccacccaacc ttcaacctgc aacctgcaaa cattcaaacc
      901 tacaacgatc accgcatggc aatggcattc tccctcattg gtctgaaatt tgatggcgtg
      961 accatcgaaa atccatcatg tgtatctaag accttcccga atttctttga agtattggaa
     1021 actttgcgat gattcgactt ggcttgatcg ggtatccact gggacattcg ttgtcaccaa
     1081 aaatccacgc agccgctctc aaagcatgca acctgcaagg tgactattcc ctattcccca
     1141 ttcaccccga agacaaacca gcattacaag ctttactcgc ccgagtccgc gctggtgaga
     1201 ttacaggact caatgtcacc attccgcaca agcagaacgt catcgaattc atggatgaac
     1261 tcacacccac tgcccaagtc atcggtgctg tcaacatgat ctacatgcgt ggcgacaaac
     1321 tcatcggcga taataccgac gcgacaggat ttctctctga cttaaagcgg gtttcaaatt
     1381 catcattcag cattcctcat tctgcactgg ttttaggtgc gggtggctca gctcgtgcag
     1441 tggtgtatgc attgctcaac gatggctgga atgtcacaat caccgctcga cgaattgaac
     1501 aagcgcaaca attagccagt tcatttacga attaccaatt acaggttgcc gatttttcat
     1561 cattcagcat tcatcattca tcgctgcttg tcaacaccac tcccctcggc atgactccca
     1621 acaccgaaac ctcacccctg cctgaaaata cggttttatc gaaaaatact tttgtttatg
     1681 atttagtgta taacccacgt gagaccaaac ttgtccgcga cgcccgcgca cagggactga
     1741 acgcctccac tggcttgggt atgctcatcg aacaagctgc cctcgccttc gaaaaatgga
     1801 caggtcacaa cccgccgcgt gatgtgttgt ttgaatccgt aggggcgacc cgccctgatt
     1861 aatcaaaacc tcggacgacc atgatgggtc gcccctattt tttggagaac ttatgcttag
     1921 attcctcacc gcaggtgaat cccacggacc atcacttacc accattcttg acggactccc
     1981 cgctgggctt tccctctcac cagacattat caacaaagaa cttgcccgcc gtcagcaagg
     2041 ctacggctca ggcggacgca
//
LOCUS       DAOQLC010000320         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_36817_length_2054_cov_16.824412, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000320 DAOQLC010000000
VERSION     DAOQLC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36817_length_2054_cov_16.824412"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1936)
                     /locus_tag="PL000_22245"
     CDS             complement(131..1936)
                     /locus_tag="PL000_22245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC91663.1"
                     /translation="MKHYRKFIILVMFALQTVSPVKAQEGLQFEGTIAFISTDGNIVL
                     ATGDGRQVRITADAGTMDPETGVEVTYDYLSFSPDGAYLAFDRDGYTGIGDGVFVYDV
                     LKGEIVRKFSFEEVGLPVLWHKDSDSFIDSEIDPSNDYQRNYYRQFITGEREGLFQLV
                     EGDNGYVINFDLGIVIYTEGNSQIPTFYDWANNQTTNLQINGLSASANGFWSPDKRLY
                     VHTGDEKVYSLINLDSGSVYKKIEIFDILPPREHTFPTGETYNLTSVEVYDLNQEGSR
                     LLLSDDLYLYELNLDTESVETVYTSRLDNPDSRLYGFWSGSGRVILIDEYLAGGSFFD
                     GVAGDQSLLVVEKGFSPTLVADDAEALFWLSGDSDQFLYKKYTQTAQQVSVELMLFDY
                     PVRESFRVGDLPTFHDISYDPYILNFIVDWTQKKIDLTENTLEPPLASPVPPNFSIEG
                     TPISGIQQNSQPSLPLPSGISASSLFYGGICCLLLAILLVIIAVFLSRSRKRKNTQSH
                     PEDQPQFVNSASGAEQVKQAIELAKAKQYHEAFEILEGVIKTEPNNVAAWFNLGRVFA
                     SMGNLKDAERCFLQARKLGHPKANEALIWLKKKSQ"
BASE COUNT          581 a          496 c          423 g          554 t
ORIGIN      
        1 tccgccgccg atgatgagaa catcgtgctg ttgcacatct gattttgtgg tcatttttgc
       61 tccattgagc tcttttcact tcatgccaac atatttgggg gaatatgata ttttattcaa
      121 cccaaaatcg tcactgggat ttttttttca accaaatcaa tgcttcattg gctttggggt
      181 gtcccaactt cctggcttgc aggaagcacc gttctgcgtc tttcaaattc cccatactgg
      241 caaataccct gccaagattg aaccatgcgg caacattatt tggttccgtc tttattacac
      301 cctccagtat ttcaaaggcc tcatgatatt gtttggcttt tgccagttca atcgcctgtt
      361 tgacctgttc cgcgccagaa gcggaattaa caaattgagg ctggtcttca ggatgggact
      421 gggtgttttt acgtttacga gagcgagaca ggaagaccgc aatgataacc agcaggatgg
      481 caagaagcag gcaacatatc ccgccataga ataaagagga tgcacttatc ccagaaggga
      541 gcggaaggct aggttgagaa ttctgctgaa taccacttat tggtgttcct tcaatcgaaa
      601 agttaggagg aacaggggag gccaatggcg gctcaagggt gttctccgtc aaatcaatct
      661 ttttttgtgt ccaatctacg ataaagttta atatataagg atcatagcta atatcatgaa
      721 aagttgggag gtcacctact ctaaagctct cacgaacagg ataatcaaaa agcatcaatt
      781 caacagaaac ctgttgtgcg gtttgagtat actttttgta caagaactgg tcggaatcac
      841 cggacaacca aaataaggct tccgcatcat ctgcgaccag agttggcgaa aatcctttct
      901 ccaccacaag caagctctga tctcctgcca cgccatcgaa gaaactgccg cctgccaaat
      961 actcatcaat caaaataacc ctgccgcttc ccgaccaaaa accatagagg cgggagtcgg
     1021 ggttgtccag tctggaagtg tatacagtct ctacactttc tgtatcaagg tttagttcgt
     1081 aaagatacag atcgtcactc aacagaagcc gtgacccctc ctggtttaaa tcatacactt
     1141 ccacactagt cagattataa gtttcgcctg tcggaaaggt gtgctcacga gggggcagga
     1201 tatcaaatat ttcgatcttt ttgtataccg accccgaatc aaggttgatc agggaatata
     1261 ccttctcatc tccggtatga acgtaaaggc gtttatcggg cgaccaaaat ccattagcac
     1321 tagcgctcaa accattaatc tgcaaattcg tagtctggtt attggcccag tcataaaaag
     1381 tgggtatctg tgaatttcct tctgtgtaga tgacaatgcc aagatcaaag ttgattacat
     1441 atccattgtc gccttcaaca agttgaaaca agccctcacg ctctccggta ataaattggc
     1501 gataatagtt tctttgatag tcgttggatg gatcaatctc agaatcgatg aaagaatcgc
     1561 tgtctttatg ccacaaaaca ggcagtccca cctcttcaaa cgaaaacttc ctgacaatct
     1621 cccccttcaa aacatcatag acgaaaacac catctccaat acccgtatat ccatctcgat
     1681 caaacgccaa ataggcccca tcgggcgaaa aactcaggta atcataggtt acttccacac
     1741 ccgtctcggg atccattgtt ccagcgtcgg cggtgatacg cacctgcctt ccatctccgg
     1801 tggcaagcac gatatttcca tccgtgctga taaaggcaat cgtcccttca aactgaagac
     1861 cctcctgggc cttgacgggc gaaacagtct gtaaagcgaa cataacaagg attatgaact
     1921 ttcgatagtg tttcatggct ttgttcccgt gagaataatg aggggtgtct attttatctc
     1981 cagtcagtat agaagcatcc tacgaataat accaactgaa gatcttgtta tacaccgcca
     2041 gcaaaatctt cttc
//
LOCUS       DAOQLC010000321         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_37293_length_2040_cov_24.840806, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000321 DAOQLC010000000
VERSION     DAOQLC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37293_length_2040_cov_24.840806"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PL000_22250"
     CDS             complement(<1..613)
                     /locus_tag="PL000_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91664.1"
                     /translation="MSKTKRWIPGVGIAIVILAAGTFLTAALLFRQALLKAGTPVYDY
                     KVVPSEHEGYSRQTLTIAGLTYESDYAEYGLSAAGNDFQVGQTSAGGRIYEIDPQRNY
                     LVVYDFMSPIGVFRNSQIPPFDWRTTDFNEMRLYSTEIATATPQEPKTSTEAQLIQAA
                     LAPMRGDSESISPSQVSGEYKSFVLYLYSEQLTGMSYAIGVYVD"
     gene            complement(652..1497)
                     /locus_tag="PL000_22255"
     CDS             complement(652..1497)
                     /locus_tag="PL000_22255"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HNC91665.1"
                     /translation="MTIQKIFIIGAGTMGNGIAQVAATSGYQVTCMDVMPAALEKAKA
                     TIAKSTGKLLEKGTITAEQKASADAIQFVSSMDTMKDADLVIEAATENPELKFKIFKD
                     MDANAREGVILASNTSSISITKIAAVTSRPDKVIGMHFMNPVPLMKLVEVIRGLGTTD
                     ETTQTIVDLCKVMGKEPVEANDSPGFISNRILCPMINEAVFALQENVGTPEAIDQVMK
                     LGMNHPMGPLTLADLIGLDVVLFVMEVLQRDLGEDKYRPAYLLRKMVDAGYLGRKTGR
                     GFYKY"
     gene            complement(1564..>2040)
                     /locus_tag="PL000_22260"
     CDS             complement(1564..>2040)
                     /locus_tag="PL000_22260"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HNC91666.1"
                     /translation="ERNEKIELYGRGYELLKAALAEVPAEAMQFKPDPKEWSVHEIIV
                     HIADSESNAALRARKLIVEPGGTIMGYDQDLWATSLKYHEHSMEDALETTRLVRKTTY
                     ELLKRQPEEVFAHFIKHPEYPDPYTFEKWINIYSGHIPGHIEQIQNNVKLWKKSVG"
BASE COUNT          413 a          553 c          526 g          548 t
ORIGIN      
        1 catccacgta cacaccgatc gcataggaca tgcctgtgag ttgttcgctg tagaggtaga
       61 ggacgaagct tttatattct ccgctcacct ggcttggaga gatggattca gaatctccgc
      121 gcatgggggc gagggccgct tgaatcagtt gagcctcggt ggaggttttg ggttcctgcg
      181 gggtggcggt ggcgatctcg gtggaataca ggcgcatctc gttgaagtct gtagtgcgcc
      241 agtcaaaggg cggaatctgg ctgttccgaa agaccccgat cggcgacata aaatcgtaca
      301 ctaccagata atttctctgc gggtcgatct catagattcg ccctcccgca gaggtttgcc
      361 caacctgaaa gtcatttccc gcggcagata aaccgtactc ggcataatcg ctttcgtagg
      421 tgaggcctgc gatcgtcagg gtctgacgcg aatatccctc atgctccgag gggacgactt
      481 tgtagtcata gacgggtgtc cctgccttga gtagcgcctg acggaagagc agggccgcag
      541 tcagaaacgt ccctgcggcc agtatgacga tggcaatgcc gaccccagga atccaccgtt
      601 tggttttaga catttcgctc tccgcatttc ttcatattgc cgtgcgaggt tttagtactt
      661 gtaaaacccg cgtcctgttt ttcttcccaa ataacctgcg tccaccatct tgcgcaataa
      721 atacgcgggg cggtatttgt cctcgcccag atccctttgc agcacttcca tcacgaacag
      781 caccacgtcc agaccgatca gatcggccag cgtgagcgga cccatcggat gattcattcc
      841 cagcttcatc acctggtcaa tggcttcagg ggtgccgaca ttttcctgca aagcaaacac
      901 cgcttcgttg atcatcgggc acaggatgcg gttcgaaatg aacccaggcg agtcgttggc
      961 ttccacgggt tccttgccca tcactttgca caaatccacg atggtctgcg tggtttcgtc
     1021 tgtggtgccc aatccgcgga tgacttcaac aagtttcatc aacggcacgg gattcatgaa
     1081 atgcattccg atcactttat cgggacggct ggtcactgcg gcgatcttcg tgatcgaaat
     1141 cgacgaagtg ttgcttgcca atatcacgcc ttcgcgcgca ttggcatcca tgtctttgaa
     1201 gatcttgaac ttcagttcgg ggttttcggt cgccgcttca atgacgaggt cggcgtcttt
     1261 catcgtgtcc atgctgctca cgaattggat cgcatctgcc gaggcttttt gctccgcggt
     1321 gatcgtccct ttttcgagca atttccccgt ggatttggca atggtggctt tggctttttc
     1381 caacgcggcg ggcatcacgt ccatgcaggt cacttggtag cccgatgtcg cggcaacctg
     1441 tgcgatcccg ttgcccatgg tccctgcgcc gatgatgaaa attttttgaa tagtcatata
     1501 gtctcctggt cgcttgtctt gtagtcggtc tgccagcctg atgaacgacc agcagacaag
     1561 cgactatccg actgattttt tccacaactt cacattattt tgaatttgtt caatatgccc
     1621 cgggatatgt cctgaataga tattgatcca tttttcgaat gtatatggat caggatattc
     1681 aggatgcttg atgaaatgag cgaacacttc ctcaggctgg cgcttgagca attcatacgt
     1741 cgtcttgcgg acgagccttg tcgtttccag cgcatcttcc atgctgtgtt cgtgatattt
     1801 caatgatgtc gcccacagat cctgatcgta gcccatgatc gtgccgccag gttcgacgat
     1861 caacttcctt gcgcggagag cggcattcga ttcactgtcc gcgatatgga cgatgatctc
     1921 atggacactc cactcctttg ggtctggctt aaactgcatg gcctccgctg gaacttccgc
     1981 cagagcggcc ttcaataatt cgtatccgcg cccgtagagt tcgatctttt cgttgcgttc
//
LOCUS       DAOQLC010000322         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_38495_length_2007_cov_26.001025, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000322 DAOQLC010000000
VERSION     DAOQLC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38495_length_2007_cov_26.001025"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1495..1608)
                     /gene="rrf"
                     /locus_tag="PL000_22265"
     rRNA            complement(1495..1608)
                     /gene="rrf"
                     /locus_tag="PL000_22265"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1726..>2007)
                     /locus_tag="PL000_22270"
     rRNA            complement(1726..>2007)
                     /locus_tag="PL000_22270"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          453 a          521 c          505 g          528 t
ORIGIN      
        1 gctgcgaata taagccaggc gtacggcaac tggctgggct gacctcgttc tcgatcattt
       61 ccccagtagc cgccacggtg tcgctgtcat tttggggaga ataggggcag ggaacagaca
      121 cttacgggct tcggcccgtt tttttcgccc gtacatcaat gaaagcccgg caacgccgga
      181 tcgctgccga gttgttgtac cgctcctttg gacgccactt ccggtagcct gtgcggcggt
      241 aatcaacacc ccttatcgcc gactcgataa gaggcactgt gcggttcctt tggacgcgtc
      301 aaatcgggag ccattgtacg actgcaatca gtgtttgaat tctaacgcaa aagcggtgcc
      361 caatacagcg caaatctctt ttgagtcatg atcattgcgg cggacttcca tttcaatata
      421 ctgtcatgat ggagctgctg atgttacttg ccttcgctgt cgtttatggc cccgatatca
      481 tatgcggtgc gctgctggca ttgctgtggg taatgagggg aatttttgag tgtgtgatgg
      541 cgatactgat aggtgggctt gagcttgtcg cttgggggct gtcccacgta gatcgcgtta
      601 cccagcccag aagaaagtaa aaccccttgt cgccgactcg atcagggggc gctgtgcggt
      661 tcctttggat gcgtcagtcg gaagccattg tacgaccgca atcaacagtg gaattctaac
      721 gccaagtcag tgccaaatac agcgcaaatc tctttcaaat atttcgcgtt ttgtttcatc
      781 ggtttctcgt gaatgtttca ttcgtttctc gggccgtcaa agattcctag tcaggttttg
      841 tgcaggacgc acgatttaac ataatataca ttatgcgcat cattggtagt ttttatgttg
      901 ttgcgtaaat catgcgaatg tgtagaagtt cagacttgat ctgacagtcg cccgttttga
      961 cggtgcccgg ttgtcagatc aattcaggtt gtcgactttc tccttccaaa cgatttttcc
     1021 ttcaatcaac gtttgcatcg gcgtgcgtcc gcagcacatc ttacttaact tggtatgagc
     1081 aacgacgaac gtaagtagaa tgacgattaa atgctgatta atagcgcttt ttttacgcaa
     1141 cagtcgacca tatctaccta gattacgcag tcacatactg aatagagcat taactactag
     1201 caatcttcct agccgacaat gcaattaaca tgacggcaca gcaattttgc gaccttgaag
     1261 ctggcatcac gcccggcgat gtgcgttccg ctgtctagac tcgacgcaac cttaatgcgc
     1321 ttaccaatga ggccaccacg cttcattccc taaccaaaaa tccgccgccg gcgcgcacgt
     1381 aagcagcctg aaacgtctcc tgcagcggat gaagcagctg cgtatcgtca accacattcc
     1441 aggataaaaa acacagagcc ccgctgattt ttcagcgggg ctgtgtataa gggagtctgg
     1501 cggtgaccta ctttcccacg ggcaatccgt agtatcatcg gcgcggaggt gtttcacggt
     1561 cctgttcggg atgggaaggc gtggtgccac ctcgctatgg ccgccagaca taagcggtag
     1621 cttgcagcat tgtagtctgc aggcagatgt ttgagcatag cgcttctgga agaagtgttg
     1681 tgtggttttg attgttcagc aaacagccga attgattcag cttataggat caagccgcac
     1741 gggcaattag tatcggttag cttaacgcat tactgcgctt ccacacccga cctatcaacg
     1801 tggtggtctt ccacgaccct tcagggcagt caagctgcca gggaagtctc atcttgaggc
     1861 aagtttcccg cttagatgct ttcagcggtt atctcttccg aacttagcta cccggcgata
     1921 cgactggcgt cataaccggt acaccagagg ttcgtccact ccggtcctct cgtactagga
     1981 gcagcccccc tcaaacttcc aacgccc
//
LOCUS       DAOQLC010000323         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_40705_length_1949_cov_27.267159, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000323 DAOQLC010000000
VERSION     DAOQLC010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40705_length_1949_cov_27.267159"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1949
                     /locus_tag="PL000_22275"
     CDS             <1..>1949
                     /locus_tag="PL000_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91667.1"
                     /translation="EPVSQTRDGITVTVTEAVLSADRTIVVVKFENVPVDKVPNQEDN
                     SDCYPAPAFQLPQGDPLQPTSSQGNDWGSGSEYHYTYAGVPADVNEATLFIGCVPGDV
                     TPRVLPENWELPLHFMVAPPDMTALPVIEYVPSPMPTDASATDSITITKVIDLGDSYI
                     LVGRFPLQTDSLGYFTPGMRSLLDGNGNFLPWKMPQDSVLPPVDPNEPFTAEDFAVQV
                     DKGFVPPLTIKVTRMSTYPAAGDTPIEYEFDAGENPKVGDDWQVNKEIQAAGYSFTLV
                     SVRADTDQDMPGMYGYRFSFKPLADPISNLYLELKGQDFMGYTQNGNIDGFTYFVGYP
                     ELPKGKLTVSFSLEINDPQAWTVQWTPDDSSANIVTPTEAPQACLTTDSWEAALANPA
                     PIPSDLTGKLVAYGRTVDDGKDPNPENTGIFTINLDGSNKQAFGQGMSPMLSPDGSQV
                     VYFDNAGLHLMDTASGVTSHIPNTVEGDIFPLWSPDGTQIAFFHGLDSDLYIIQPDGS
                     GLRRALQGIDAEQLVGWTSDSRGLYYYVNTETGSELRSVDIESEGVTSLFEHDGYAFF
                     NASPDGSKIAFVGHPNNLYISNTDGTGRELLGFMGNLFISTPLAWSPDGKWVIVTIRR
                     LEDPDIAGLPIPHALVSVTNCQIIP"
BASE COUNT          446 a          579 c          500 g          424 t
ORIGIN      
        1 ccgaacccgt ttcacagaca cgcgatggaa ttaccgtcac tgtgacggaa gctgtactca
       61 gtgcagaccg taccattgtg gtggtcaagt tcgaaaacgt acccgtggat aaagtcccga
      121 atcaggaaga taactctgat tgttatcctg cgcccgcatt tcaactgcca cagggtgatc
      181 cgcttcaacc cactagctcg cagggaaatg actggggctc gggcagtgaa tatcattaca
      241 cctacgcggg cgtacctgcg gacgtgaacg aggcgacgct cttcattggc tgtgtgccag
      301 gcgacgtgac accgcgcgtc ctgcctgaga attgggaact accgttgcac tttatggtcg
      361 ccccgcccga tatgaccgcc ttgcccgtga tcgaatatgt tccttcgccc atgcccaccg
      421 acgcttccgc gacggactcc atcaccatca ccaaggtgat tgacctcggt gacagttata
      481 tcctcgtggg caggttcccg cttcaaactg attcgcttgg atatttcact cctggtatgc
      541 gctcgttgct ggatggcaac ggaaacttcc tgccgtggaa aatgccgcag gattcggtgc
      601 tcccgcctgt tgatccgaac gaaccgttca cagccgaaga ttttgctgtc caagtggata
      661 agggttttgt cccgccgctg accatcaagg tcacaaggat gtccacgtat cccgccgcgg
      721 gtgacacgcc gatcgaatac gaatttgacg cgggcgaaaa tccaaaggtc ggtgatgatt
      781 ggcaggtcaa caaagaaatt caggccgctg ggtattcgtt cacgctggta tccgtccgcg
      841 cggacacgga tcaagatatg ccaggcatgt acggctatcg tttcagcttc aagcccttgg
      901 cagacccaat ctccaatctc tacctggaac tcaaaggtca agatttcatg ggctacaccc
      961 aaaacggcaa catagacggc tttacgtact tcgtaggcta tcccgagttg ccgaaaggta
     1021 aactgaccgt ttcattcagc ctggagatca acgatccgca ggcatggact gtgcaatgga
     1081 cacctgacga ctcttccgcg aacatcgtca ccccaacgga agcgcctcaa gcctgcctga
     1141 caacggattc gtgggaagcc gcattagcca accccgcacc gattccctcc gacctgacag
     1201 ggaaactcgt cgcctacggc cgcaccgtgg acgatggcaa agacccgaac cctgaaaaca
     1261 caggcatatt caccatcaac cttgacggaa gcaacaagca ggctttcggt cagggcatga
     1321 gtcccatgct ctcaccagat ggttcgcagg tggtttactt tgacaacgca ggtttgcact
     1381 tgatggatac cgccagcggt gtgacgagcc atatccccaa cacggtcgag ggcgatatct
     1441 tcccgctctg gtcgcccgat ggaactcaga ttgccttctt ccacggcctc gactctgatt
     1501 tgtacatcat ccagcccgat ggaagcggac tccgccgcgc cctgcagggc attgatgccg
     1561 agcaattagt cggttggaca tccgacagcc gcggcttgta ttactacgtc aacaccgaga
     1621 caggctcgga actccgcagt gtggatattg aatccgaggg cgtgaccagc ctgtttgaac
     1681 atgacggtta cgctttcttc aatgcttcgc ccgatggcag taagatcgcc tttgttggcc
     1741 accccaacaa cctgtacatt tccaatacgg acggcacagg ccgcgagttg cttgggttca
     1801 tgggcaacct attcatttcc acacctttgg cctggtcacc cgatggcaag tgggtaatcg
     1861 taaccatccg cagactggaa gatcccgata tcgccgggct accaattccg cacgcgttgg
     1921 ttagcgtgac aaactgccag atcatcccc
//
LOCUS       DAOQLC010000324         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_41186_length_1936_cov_31.238171, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000324 DAOQLC010000000
VERSION     DAOQLC010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41186_length_1936_cov_31.238171"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..522)
                     /locus_tag="PL000_22280"
     CDS             complement(286..522)
                     /locus_tag="PL000_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91668.1"
                     /translation="MKLSKSHLDMISGSAPGELEVVLHPTKPLIIIGVVGTAVLMTMD
                     PKQVEVLEKRLAQVRGMLRDKALVEKSTPSTVGQ"
     gene            complement(488..>1936)
                     /locus_tag="PL000_22285"
     CDS             complement(488..>1936)
                     /locus_tag="PL000_22285"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNC91669.1"
                     /translation="CCVTKELIDGYYTRLLAERRVTSAGDTTALDFYLRHYAEVFRPL
                     LELMAAGVRVDSTQRARRYDELVGRVSELKTQIVGAGADIFSTGKTAKGGISRKKLGT
                     YLYETLGLPKQFVKDKKKGEKKVTTNEIAIRKLAHQFPDKLGQVAPLVLDQVRCKKLS
                     EFYVEERIDPDGYFRSSYGLNTEAGRLNSKESPRGTGSNAQNIDREARDMFLADPGCV
                     GVEVDLSQAEARIVYLAIYRLTGDRDMLNKARLRPDEYDQHTEMAAKIFGKAPHEVTK
                     LDRYLGKTTVHAAQREQSGKGLSERLAKDGYFYDQRECDGFIKGYKKAVPGLEDYFRW
                     VRGRIISDRMLMTDWGRILRFDYDRLDNSIYRAGYSFDPQANVADHMNQKGLVPFHWW
                     LVDNKCGRINVHAHDALFFSVRPEWAYEATKFLVESLESEFTYHGTPLMMPCGIKCGH
                     SWKGKQEWKRLPAQSQFEEVVYEIVEKSLGHD"
BASE COUNT          408 a          604 c          444 g          480 t
ORIGIN      
        1 gggtttactt tggattgctg ctccgcgacc aatttgagga ctactttgat cgcctgctct
       61 ttcccactcc ccgacggccc caccaaaaac acatacaaat tgggataaat tttggtggcg
      121 atgtccgcct tcacccacac ccgatccgcc acacacgccg ccagcagact catacagctc
      181 cagaaatgaa acctccgcgg gacttcgctt ctcccgatca tgtgcatata gaggtcaata
      241 aattggcgct ccccctcatc caagatcccc acgctgcgcc ccctttcact gccccacggt
      301 cgacggggtg gatttctcga cgagtgcttt gtcccgcaac atccctctca cctgcgcgag
      361 ccgcttctcc agcacctcca cctgcttcgg gtccatggtc atcaacaccg cggtcccgac
      421 caccccgatg ataatcagcg gtttggtggg atgcagcacc acctccaact cccccggcgc
      481 actgccgcta atcatgtcca agtgactttt cgacaatttc atacaccacc tcctcaaatt
      541 gcgattgtgc gggcaaccgt ttccattctt gcttgccctt ccaactgtgc ccacatttta
      601 ttccgcacgg catcatcagc ggggtgccgt gatacgtaaa ttcactctcc aaactttcca
      661 cgaggaactt ggtcgcctcg tacgcccact ccggccgcac cgagaaaaac agcgcatcgt
      721 gagcatggac attgatccgc ccgcacttgt tatcgaccag ccaccaatga aacggcacca
      781 gccccttctg gttcatgtga tccgcgacat tcgcctgggg gtcaaaactg tacccggcac
      841 gatagatcga attatccaac cgatcataat caaacctgag tatcctcccc caatccgtca
      901 tcaacatccg gtcacttata attcgtccac gcacccaacg gaaataatct tccaatcctg
      961 gtaccgcttt tttgtagcct ttgatgaacc cgtcgcactc gcgctggtcg tagaagtacc
     1021 catccttcgc gagccgttca ctaagccctt tcccactttg ctcccgctgc gccgcgtgga
     1081 cggtggtttt gccgaggtag cggtcgagtt tggtaacctc gtgaggagct ttgccaaaaa
     1141 tttttgccgc catttcagta tgctgatcgt actcgtcagg tcgcagtcga gccttattaa
     1201 gcatgtctcg atcgccagta agtcgataga tggcgaggta gacgatccgc gcctccgctt
     1261 ggctgagatc cacttcgacc ccaacacacc ctgggtctgc gaggaacatg tcgcgagctt
     1321 cgcgatcgat attttgagcg ttcgatccag tgccgcgagg agattctttg gagttgagcc
     1381 ggccggcttc ggtgttgagg ccatagctcg atctgaaata cccatcaggg tcaatccttt
     1441 cttccacata gaactcactc agcttcttgc accgcacctg atccaagaca agcggcgcca
     1501 cctgcccgag cttgtcaggg aattggtggg cgagtttgcg gatggcgatt tcgttggtgg
     1561 tgactttctt ttcgcccttc tttttgtcct tcacgaactg cttgggaagt ccaagtgttt
     1621 catagaggta ggtaccgagt ttttttcggc tgatgcctcc tttggcggtt ttgccggtgg
     1681 agaagatgtc tgctcccgct ccaacaattt gtgtcttaag ttcagatact cgcccaacaa
     1741 gttcatcata acgtcgggca cgttgggtac tatcaacacg aacccctgct gccataagct
     1801 ccaagagagg tctaaatact tccgcgtagt ggcggaggta gaagtcgagg gcggtcgtgt
     1861 cacccgcact tgtaactcgc cgttctgcca acaatcgtgt gtagtatcca tcaatcaact
     1921 ccttcgtcac acaaca
//
LOCUS       DAOQLC010000325         1932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_41366_length_1932_cov_20.429409, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000325 DAOQLC010000000
VERSION     DAOQLC010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41366_length_1932_cov_20.429409"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PL000_22290"
     CDS             complement(<1..330)
                     /locus_tag="PL000_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91670.1"
                     /translation="MKITKDQLKCWKACQDGYGWFLRRFPDGEAEYQDVLNALAEDDR
                     PADADWLMNHAGPDRESVLEVEVIADCKHFFASGRLVIKTSATVAGQLTAGDGIEAGT
                     CINAGWGI"
     gene            complement(362..664)
                     /locus_tag="PL000_22295"
     CDS             complement(362..664)
                     /locus_tag="PL000_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91671.1"
                     /translation="MADLNCVYKGFEFWLEQKLLDKRGSFEIRPSQIAWHIRRKRAGS
                     KTFVLGRDLSELRLFVLSDDLDSWRVVFRTSKPFDYDGLLREIMKHRDIQESLSDV"
     gene            complement(684..1223)
                     /locus_tag="PL000_22300"
     CDS             complement(684..1223)
                     /locus_tag="PL000_22300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91672.1"
                     /translation="MEKRPGVKRHNFCDLDIQDIRCFVVYIEEGTSTAAGKRLNMTQA
                     NVIYRAKVIQKHVGIPMYLESATHALPHSGGRHFTDLTELGKIVFTILKDILQRYDNL
                     VELMRAYNPSPGNTRMETTHGVPFNMNPAIPQTAEAEVDAVTVYDQIRTNFPGVFGEG
                     NVNFYPVKEQLAKRITRTD"
     gene            complement(1225..>1932)
                     /locus_tag="PL000_22305"
     CDS             complement(1225..>1932)
                     /locus_tag="PL000_22305"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNC91673.1"
                     /translation="PLVRGIFVADDGYDFGMFDYSQQEPRVTVHYGYLRGYKGADEAR
                     EKYVTNPRTDYHQLVADLMEQASGRAWGRKVAKPINLGLAYGMGKNKLAAQLGVAVDE
                     SGPILDAYHKAVPFVRMLGDEATRQAKRRGYIRTIMGRRRHFPGGEFAHKALNAAVQG
                     SSADMVKKAIVEVHEAGHTIYNTVHDEIDCPVRVGDRKHAAEIRDILINAMKLEVPLV
                     VDVEVGPSWGEHELIEV"
BASE COUNT          427 a          536 c          498 g          471 t
ORIGIN      
        1 gatgccccat ccggcattga tgcacgtgcc ggcctcgatg ccatctccgg cggtcaactg
       61 tccggcgacg gttgcgctgg tcttgatgac gaggcggccg gaggcgaaaa agtgcttgca
      121 gtcggcaatg acctccacct ccagcaccga ctcgcggtcg gggccggcgt gattcatcag
      181 ccagtcggcg tcagcgggac ggtcatcctc ggccagggcg ttgagcacgt cctgatactc
      241 tgcctctcca tccgggaagc ggcgcaggaa ccacccgtag ccatcctggc aggctttcca
      301 gcatttcagt tgatccttgg tgatcttcat tgagattctc cttcacgatt aggaagttat
      361 attatacatc ggataatgac tcttgaatat cccgatgttt cataatttcg cgcaatagtc
      421 catcatagtc gaagggcttg cttgtgcgaa acacgacacg ccaagagtca aggtcatcgg
      481 ataaaacaaa cagacgcaac tcagacaggt cacgcccgag tacaaaggtt tttgagcctg
      541 cccgtttgcg gcggatatgc cacgcgattt gcgaaggacg aatttcaaat gacccacgct
      601 tgtcgagtag cttttgctct agccagaact caaatccctt gtacacgcag ttcaggtctg
      661 ccaagcctga accggctact gactcaatcc gttcgagtaa tacgtttggc cagttgttct
      721 ttaacagggt agaaattgac gtttccttca ccgaacaccc ccggaaaatt agttctgatt
      781 tgatcgtaga ctgtcacagc atcgacttca gcctcggctg tttgtggaat tgctgggttc
      841 atgttgaacg gtaccccgtg cgtggtctcc atccgggtat tacctggcga tgggttatac
      901 gcccgcataa gttcaactag gttgtcgtac cgctggagga tgtccttcag aatcgtgaac
      961 acaatcttgc cgagttcagt gaggtccgtg aagtgccggc cgccagaatg aggtagcgca
     1021 tgggttgcag actccagata cattggtatg ccaacgtgct tctgaatgac ctttgctcta
     1081 tagattacgt ttgcctgtgt catattcaga cgtttgcccg cagccgtaga cgtaccttct
     1141 tcaatgtaga caacaaagca tcgaatatcc tgaatgtcca gatcacagaa gttgtggcgc
     1201 tttacgccag gtcttttttc catgctacac ctcgattagt tcgtgttcgc cccagctcgg
     1261 accgacttca acatcgacca caagcgggac ttccaatttc atggcgttga taaggatgtc
     1321 gcgaatctcc gcagcatgct tgcggtcgcc aacacgaaca gggcagtcga tttcgtcatg
     1381 gacggtattg tagatcgtat gcccagcctc gtgtacctca acgatagcct tcttcaccat
     1441 gtccgcggag ctgccttgaa ctgcggcgtt caatgccttg tgtgcaaact cgccaccagg
     1501 aaagtgccgt cgtcgaccca taatagttcg gatgtacccg cgacgcttag cttgtcgtgt
     1561 agcctcatcc ccaagcattc gcacaaatgg aacagcctta tggtaggcgt caaggatggg
     1621 acctgactcg tcaactgcaa cgccgagttg agccgccagc ttgttcttgc ccataccgta
     1681 agcaaggccc aggttaattg gctttgccac cttacggccc caggctcgac cagatgcttg
     1741 ctccatgagg tctgcaacta gctggtggta gtcagtgcgc ggattggtga cgtacttctc
     1801 tcgggcctca tcagcccctt tgtacccgcg aagatagccg tagtgcacgg taacacgagg
     1861 ctcttgctgc gagtaatcga acatgccgaa gtcgtagcca tcgtctgcca caaagatacc
     1921 gcggaccaag gg
//
LOCUS       DAOQLC010000326         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_41738_length_1922_cov_25.654526, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000326 DAOQLC010000000
VERSION     DAOQLC010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41738_length_1922_cov_25.654526"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PL000_22310"
     CDS             complement(<1..1154)
                     /locus_tag="PL000_22310"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91674.1"
                     /translation="MMNLFPWVMAGLLLLVAAWFAWRYSVLRRRINEYSIVVRDHSGN
                     LPTDFKSLETLSNTVASLKTTFDLQFSTLNSQNTRLATVLSQLTDGVLIADSMGQVQY
                     ANPAAQKLFESENPIGRSVAEVVRNHQLIEAWRRCQQVRELQTESVELPARKYFLQLI
                     VIPDEHAGGSLLVVQDLTRVRKLETVRRDFISNVSHELRTPLASLKALTETLQNGAIA
                     DPEAGPRFLSRITTEVDALTQMAQELLDLSRIESGQVGLVLAPLSPKDLLSSAADRMK
                     LQAERANLKLSANCPAGLPAIRADQSRLEQVLVNLIHNSVKFTKPGGEIVLEAEAGYS
                     CVRFAVRDSGVGIPSESLSRIFERFYRVDRSRTGSGTGLGLSISKHIVEA"
     gene            complement(1169..1879)
                     /locus_tag="PL000_22315"
     CDS             complement(1169..1879)
                     /locus_tag="PL000_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91675.1"
                     /translation="MAETILVVEDELSLQETLAYNLKKEGYTVETVGDGRSALESARR
                     LKPDLIVLDIMLPEMDGFEVARILRKEMSTAILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELMARVKSQLRRARLLREEMGRPEASPPRHEALKFDNLVINLTRREVTLDETP
                     LQLKPKEYELLLFFAEHKGQMLSREFVLERVWGWDFIGDSRTVDVHIRWLRQKIEKDS
                     ANPVRIVTVRGGGYRFEG"
BASE COUNT          448 a          545 c          486 g          443 t
ORIGIN      
        1 tgcgcctcca caatatgctt cgagatcgaa agcccaagcc ccgtgcccga acctgtgcgg
       61 gaacgatcca cgcgatagaa tctttcaaag atgcgggata gactctccga agggatcccg
      121 actcccgaat cccgcacagc gaatcgtaca cagctgtaac ctgcctctgc ttcgaggacg
      181 atctctcccc cgggctttgt gaacttcacc gaattgtgaa tcagattcac caatacctgc
      241 tccagccgcg attggtccgc gcggatggcg ggcaggcctg cagggcagtt tgcgctcaac
      301 ttcaagtttg cgcgttccgc ctgcaatttc attcgatctg ccgcagaaga caacaaatcc
      361 ttcggtgaca acggcgcaag gaccaacccc acctgccccg attcaattct ggaaagatca
      421 agcagttcct gcgccatttg cgtcagcgca tccacttcgg tggtgatcct gctcaagaaa
      481 cgcggacccg cttcagggtc tgcaatggcc ccgttttgca gggtctcagt caatgctttg
      541 agtgatgcaa gcggggtccg cagttcgtgt gagacgttcg aaatgaaatc cctgcgcacg
      601 gtttcaagtt tacgcacccg ggtcaggtcc tgcaccacca gcaggcttcc acctgcatgt
      661 tcatcgggaa tgacgatgag ctgcaaaaaa tattttcggg cgggcaattc aacagattcg
      721 gtctgcaact cgcgcacctg ctgacagcgg cgccatgcct cgatcaattg atggttgcga
      781 accacttcag ccacactgcg cccaatgggg ttctctgact cgaaaagttt ttgcgctgca
      841 gggttggcat actgcacctg tcccatcgaa tccgcgatga gcacgccgtc ggtcaattgg
      901 gaaaggacgg ttgcaaggcg ggtgttttgt gagtttaaag ttgaaaattg aaggtcgaag
      961 gttgttttga gcgaagcaac tgtgttggaa agggtttcaa gggatttgaa atcggtgggc
     1021 aaatttccag aatggtcgcg gacaacgatc gaatactcat taatccgtct tcttaaaaca
     1081 gaatatcgcc acgcaaacca tgcagcgacc aaaagcaaaa gaccagccat tacccacgga
     1141 aatagattca tcatcgctca tttccccgct atccttcaaa cctgtacccg ccgccgcgca
     1201 cggtcacgat ccgtaccggg ttggcagagt ctttttctat cttttggcgc agccagcgga
     1261 tatgcacatc cacggtgcgg ctatcgccga taaaatccca gccccacacg cgttccagaa
     1321 caaattcacg cgaaagcatc tggcctttgt gttcggcaaa gaacagcagc aattcatatt
     1381 cttttggttt taattgcagg ggggtttcat ccagcgttac ttcgcggcgg gtaaggttaa
     1441 tgacaaggtt atcgaatttc aaggcctcat gcctgggagg tgaagcctcc ggcctgccca
     1501 tttcctcacg cagcaagcgc gccctgcgaa gctgcgactt cacccgcgcc atcaactcgc
     1561 gcatggagaa cggtttggtc aggtagtcat ccgccccgac ttcgagcccc accacacggt
     1621 caatttcatc gtcacgggcg gtgagcatca aaatcgccgt ggacatttcc ttgcgcagaa
     1681 tccgtgccac ttcaaagccg tccatttcgg ggagcatgat atcgagcacg atcaggtcgg
     1741 gtttcaagcg ccgcgccgat tcaagcgcag atcgtccatc ccccaccgtt tccaccgtgt
     1801 acccttcttt tttcagattg taggcaaggg tttcctgcaa agagagttca tcttcaacaa
     1861 caaggattgt ttcagccatt gctgggaata tatcacaaag ggttggctgt ctgttaatgg
     1921 ga
//
LOCUS       DAOQLC010000327         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_41866_length_1919_cov_21.018777, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000327 DAOQLC010000000
VERSION     DAOQLC010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41866_length_1919_cov_21.018777"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1468)
                     /gene="ltrA"
                     /locus_tag="PL000_22320"
     CDS             complement(80..1468)
                     /gene="ltrA"
                     /locus_tag="PL000_22320"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HNC91676.1"
                     /translation="MGGKRQKIPNMVFGTSAGGEAADHVLEGTEPRMTETDPESLAGT
                     GRWMEEVLDKENLKVALKRVIKNGGAPGIDGMTTEELPEFLKANWQRIRSQLLEGSYK
                     PKPVRRVEIPKPGSEKKRKLGIPSCLDRFIQQALQQILQKHWDGTFSNHSYGFRPQKS
                     QHQAIAKAQEYITQGYRYVVDFDLASFFDRINHDVLMGLVTKRVKDRRVIKLIRAFLN
                     AGVMENGLVKPTEEGAPQGGPLSPILSNLMLNELDKELERREHKFVRYADDSNVYVRS
                     KRAGERVMASVTKFLEKKLRLQVNKEKSAVARPWERKFLGFSFTNNKAPKRRIAPTAL
                     EQVKQRIREITSRKRGNTLQQVIQELHTYLTGWVGYFGYCQTPSTLEHLEKWIRRKLR
                     CMIWKRWKNGKTRYRELRKLGIKKTQAAEGAGDGKHGPWHMSRTPPLNSALSKGYFES
                     LGLPRLCCRSAA"
BASE COUNT          332 a          566 c          423 g          598 t
ORIGIN      
        1 atcggcatag gggggacctc acggtccctc cctgccacac caccgtacaa gcgggtccgc
       61 atacggcggt tcaataggct taagcggcag agcgacagca gagtcgtggt agcccaaggg
      121 attcaaagta cccttttgag agcgccgaat ttaatggggg tgtcctgctc atatgccagg
      181 gtccgtgctt gccgtccccg gctccttctg ctgcttgagt tttcttaatc ccaagctttc
      241 ggagttctct gtagcgggtc ttcccattct tccagcgctt ccatatcata catcgcagtt
      301 tcctgcgtat ccacttctcc agatgttcaa gagtgctcgg agtttgacag tatccaaaat
      361 accccaccca tccggttagg tatgtgtgta gttcttggat tacctgctgg agggtgtttc
      421 ccctcttgcg gctcgtgatt tctctgattc gttgtttcac ctgctccaat gccgtcggtg
      481 ctattcgccg ttttggtgcc ttattatttg tgaagctgaa tccgaggaac ttcctttccc
      541 atggtcgtgc cactgcgctc ttctctttgt ttacctgtag tcgcaatttc ttttcaagga
      601 actttgtgac acttgccatt actctctctc cggctctttt gctgcgtaca tacacattac
      661 tgtcgtccgc gtaacggacg aatttgtgtt cccgcctctc cagttcctta tcgagttcgt
      721 ttagcatcag gttacttaaa atcggcgata gcggtccgcc ctggggcgcg ccttcttccg
      781 ttggtttcac tagcccgttc tccatcacac cggcattcaa gaatgctcga atgagtttta
      841 tcacacgccg gtctttcact cgctttgtca ccaaccccat taatacgtcg tggttgatcc
      901 tgtcgaagaa actcgccaag tcaaaatcta caacgtaccg atagccctgc gttatgtact
      961 cttgcgcttt tgctatcgcc tgatgctggg atttctgtgg gcgaaatccg tagctgtgat
     1021 ttgagaacgt tccgtcccag tgcttttgaa ggatttgctg taaagcttgt tggatgaagc
     1081 gatcgaggca gcttggtatt cccagtttgc gcttcttctc gcttcccggc ttaggtattt
     1141 ctacccttct aaccggtttt ggcttgtagc ttccttctaa caattggctt cggattcttt
     1201 gccagtttgc cttgagaaac tccggcaatt cttcggttgt catcccgtct attcccggtg
     1261 ctcctccatt cttgattact cttttgagtg ctactttgag gttctctttg tctaacactt
     1321 cctccatcca tcgtcctgta cccgccagac tttcggggtc tgtctccgtc atgcgcggtt
     1381 cagtcccttc aagcacgtga tcggcggctt caccgcctgc actcgtgccg aagaccatat
     1441 ttggtatttt ctgccgcttt ccacccatgt ggtaccagtc ctactcgtct tgctctatcg
     1501 ttcggacctt cgatatcttc ctccggctgc ctgcgtactt ccgcgcttgc tcatcggcta
     1561 tatatctact ttgtcctctg ctgactcctg cactgcaatc gagcacctct caatactctt
     1621 agtctcggaa ttccaagaca cagtgcaggc ctcccggggt aagtccaaca gctttctccg
     1681 cacaactgcc gaatctacag ccgcacccct tgatgacttt ggactttgca atcattggct
     1741 tgctcgtccg gttacggctg ccttgtattc ggtttctgtt cgtcagctcg cagatttgct
     1801 ctacgcttcc ttcagaccca gcctcgcggc tacgcccttg cgcttcacta cagttaccgt
     1861 catcaggtcc tgagaagtct ttcacctcct agttgttgaa catgcccggc gcaccaaaa
//
LOCUS       DAOQLC010000328         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_42758_length_1898_cov_24.780792, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000328 DAOQLC010000000
VERSION     DAOQLC010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42758_length_1898_cov_24.780792"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..263)
                     /locus_tag="PL000_22325"
     CDS             complement(63..263)
                     /locus_tag="PL000_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91677.1"
                     /translation="MNDEIIVEVRAIKEALAEESGFDIRRIAEEVRKSEVRSAAEGWK
                     HIPAPLQPLPNSAFQRTRFARR"
     gene            complement(256..744)
                     /locus_tag="PL000_22330"
     CDS             complement(256..744)
                     /locus_tag="PL000_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC91678.1"
                     /translation="MENPRPSVYVETSIVSYLSARESSSLLGAAHQLVTRRWWDRRHF
                     YRCFVSDVVIRECRAGDPTAALRRLDAVREFSSLAINDQAIEIAESLLALNIVPKKAA
                     EDALHVAIATAHGMDFLLTWNCRHIANPIIQARIAAHLDSIGLLLPFICTPEELLGEE
                     SE"
     gene            complement(1002..1799)
                     /locus_tag="PL000_22335"
     CDS             complement(1002..1799)
                     /locus_tag="PL000_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91679.1"
                     /translation="MKTATSVLCRIVCTMIFVWPGLSFPQEQANARLQAELVSPWLVT
                     VQGQDRPRTLRITGVRQDPGGKLILDAAFGWTDGNQVSVPTASVIQSDQETRLLFVTQ
                     AGTQIAAVLTSSDLFDGTFTTKDGVVKTVRIQKLTEEDLRAKIAATKAARAARIVVKP
                     SADVPESCAAFSGQWVGRWSQGNIGKSWLWVAEIEVNCIAKVSVERSDSPPNSFITVE
                     IKDGELSFLCNSSTGGTCVFKRNGNDLWASYSNPSGGKNNAVFERVR"
BASE COUNT          430 a          561 c          486 g          421 t
ORIGIN      
        1 cacacggacc tcctttccaa agtttgtagc cggagcgcgc ctgcgatgcc caacgtccaa
       61 attcagcggc gagcgaagcg agtccgctgg aatgcagagt tgggcaacgg ctgtaacggg
      121 gccgggatgt gtttccagcc ctccgctgcg cttcgaactt ccgattttcg aacctcttcg
      181 gcaatgcgcc ggatgtcaaa accagattct tcggctaacg cctctttgat cgcccttacc
      241 tcaacgatga tctcgtcatt cactttcttc tcctagcagt tcttccggtg tgcagatgaa
      301 tggtagcagc aaaccaatgc tgtccagatg cgcagcgatt cgtgcctgaa ttatcggatt
      361 ggcgatgtgg cggcagttcc atgtcagcaa gaaatccatg ccatgggcag tagcaattgc
      421 aacatgcaag gcatcttcag ccgccttctt tggaacaatg ttcagagcca gcagtgattc
      481 cgcaatctca atggcttggt cgttgatagc caatgacgaa aactcgcgca cggcgtccaa
      541 tctacgaagg gcggcagtcg ggtcacctgc cctacattct ctgataacca catcagacac
      601 aaaacaacgg taaaagtggc gacgatccca ccacctccgg gtgaccagtt gatgagccgc
      661 gccaagcaag gaactactct ctcgcgcaga aagatagctc acgatggagg tttcaacgta
      721 gaccgatggc cgtggatttt ccatacttac acattaagaa cagtagtcaa ttgttccacc
      781 tttgccgtct cgccagcacc gacttttcgg aaaatcgacc ctggcccctt tcggccccgc
      841 cgaccgcatc acggcgtcag cgcgccagcc aaaggcgccg cgcgaatgac ggcagcgaag
      901 gaggtgcctc ttgtccaacg ggtcagttca cgcacgcggt tcttgagttt gtcaacagat
      961 tgtggccagt ccgttcgccc attgatgggc atgagttcca ctcacctgac ccgttcaaaa
     1021 acggcattgt tcttgccgcc ggaagggttg gaatagcttg cccaaagatc attcccgtta
     1081 cgcttgaaaa cgcaggtgcc accggttgat gaattgcaca gaaaggacag ctcgccatcc
     1141 ttgatttcga cggttatgaa gctgttaggg gggctgtcac tccgctcgac agacaccttt
     1201 gcaatgcaat tgacctcgat ttcggcgacc catagccaac tcttaccaat attaccctga
     1261 ctccaccgcc caacccactg accgctgaac gctgcgcacg attcggggac gtccgcggag
     1321 ggtttgacca cgattcttgc cgcccgagcg gccttggtgg ccgctatctt cgcccgcagg
     1381 tcttcctccg tgagcttttg gatccttact gtcttcacaa ccccatcctt agttgtgaag
     1441 gtgccatcaa atagatcgct cgacgtcagg acggccgcga tctgagtgcc ggcttgtgta
     1501 acaaacagca gcctagtctc ttggtcggat tggattacgg aagccgtggg aaccgagact
     1561 tgattcccat cggtccaccc aaaggcggcg tcaagaatca atttgccgcc ggggtcttgt
     1621 ctcactcccg taatcctgag cgtccgtggt ctgtcttgtc cttgcactgt aaccaaccat
     1681 ggggagacca attccgcttg caaccgggca ttagcctgct cctgcgggaa cgaaaggcct
     1741 ggccagacga aaatcatcgt gcagacgatg cgacagagaa cgcttgtggc ggttttcatg
     1801 agaagaaacc tcccgtgtga atgaaaagaa ccccccgtgt gaagcccaat gctctcaacc
     1861 tagcacgcca taggcataat tgcatcagcg gccggcaa
//
LOCUS       DAOQLC010000329         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_42930_length_1894_cov_13.501903, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000329 DAOQLC010000000
VERSION     DAOQLC010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42930_length_1894_cov_13.501903"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1894)
                     /locus_tag="PL000_22340"
     CDS             complement(66..>1894)
                     /locus_tag="PL000_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91680.1"
                     /translation="VIDAVDCTGETCVVENIVATLTVKFPGIAGVHTYVKVNDGVAGA
                     SGGDVENRTYKNDEASMVVLKNTYDVVVVKGAKQKVIDAVDCTGETCVVENIVATLTV
                     KFPGISGVHTYVKVDNGVAGTATGGDVENRTYKNNETSMVVLKNTYDVIVVKGAKQKV
                     IDAVDCTGNTCVIEDIVATFTLKFPGKTGVHVYVKVIDNVAGTAAGGDVENRTYQNNS
                     TTMVLLKNTYDVVVKIGADSYILDDVNCTGNTCTYSLAVVKLVNSTNAALAGGVVKYY
                     DGSWHPVGNTNASGLMAFGYPGTSTNLLFSVDYAFTHNEKWQNIGTNSTVLFQTKKVE
                     VQLKNSVGTLVDAGVVKYYTGAWHDFGNTSGGKVSMELLPSNVLFSMDNQFVRNEKWQ
                     NTTTDATVVFQMKNVEVQLKDSSGTLMDTGTVKYYTGAWHELGSTSGGKVNVDLLPAN
                     ILFSMDNQFVRNEKWQNTGSNATVLFQTKKVVVQLKDSTGALMDTGTVKYYTGSWHTF
                     GTTSGGEVSMELLPANILFSMDYAFTRAEKWQNTGPDETVVFQTGKVTSATGTCTQYY
                     TGSWHAFTNGMELLPANVLFQFNDGNPQVWFTPAAGVANNIH"
BASE COUNT          429 a          518 c          448 g          499 t
ORIGIN      
        1 gaatattcga aaataaaaat gtgccccgaa aattcggggc acatttttca agcaaatgcg
       61 ttctgttaat ggatgttgtt tgccactcca gccgcaggcg taaaccagac ctgcggattc
      121 ccgtcattga actggaagag gacattggcg ggcagtaact ccatgccgtt cgtgaaggcg
      181 tgccaactgc cagtgtaata ctgggtacaa gtaccagtgg ccgaagtcac tttgcccgtt
      241 tggaaaacga cagtttcgtc agggccggtg ttctgccatt tttctgcacg ggtaaaggca
      301 tagtccatgc tgaacaaaat gttggcaggc agcaattcca tgctgacttc accgccagag
      361 gttgtgccga aggtatgcca tgaacctgtg tagtatttga ccgtgccagt atccatcagg
      421 gcaccagtgc tatccttcaa ttgcaccacg actttcttgg tctggaataa tacggtcgcg
      481 ttgctgcctg tattctgcca tttttcgtta cggacgaatt gattatccat gctgaacagg
      541 atgttcgcag gcagtaaatc cacattcact ttgccgccgg aagtgctgcc aagctcatgc
      601 catgcacctg tgtaatattt gaccgtgccg gtgtccatca gcgtgccgct gctgtccttc
      661 aactggactt caacgttctt catctggaag acaaccgtgg catctgtagt ggtattctgc
      721 catttttcat tgcgcacgaa ctgattgtcc atgctgaaca ggacgttgga aggcagcaat
      781 tccatactga ccttgccgcc ggaggtattg ccaaagtcgt gccaggcacc tgtgtaatat
      841 ttgaccacgc cagcatcgac gagggttcca acgctgttct tcagctgtac ttcaaccttt
      901 ttagtctgga acagaacggt cgaattggtg ccgatattct gccacttttc gttgtgcgta
      961 aacgcataat ccacgctgaa cagcaggttg gttgatgtcc cagggtaccc aaaagccatc
     1021 agtccgcttg catttgtatt gccaacggga tgccaactgc catcataata tttcactaca
     1081 cctccggcca gcgccgcgtt cgtgctattg accagtttga ccacagcgag gctgtaggtg
     1141 caagtattcc cggtgcagtt gacatcatcg aggatatagg agtctgcgcc gatcttgacc
     1201 accacgtcgt atgtgttctt gagcagtacc atcgtagtgg aattgttctg ataggtgcga
     1261 ttctctacat cgccgcctgc ggctgtgcca gccacattgt cgatcacttt gacatagaca
     1321 tgtacaccgg tcttaccggg gaatttgagc gtaaaggttg caacaatgtc ttcgatcaca
     1381 caggtattcc ccgtgcaatc caccgcatca atgaccttct gcttcgcacc cttgaccacg
     1441 atcacatcat aggtgttctt caaaaccacc atggaagttt cgttgttctt gtaagtacgg
     1501 ttctccacat cgccgccagt ggctgtgcca gcaacgccgt tatccacctt cacataggta
     1561 tggacgccgc tgataccggg gaatttcact gtcagtgtag cgacgatatt ctccactaca
     1621 caggtttcgc ccgtgcagtc caccgcatca atgacctttt gcttcgcacc cttgaccaca
     1681 accacatcat aggtattctt cagcaccacc atggatgctt cgtcattctt gtaggtgcgg
     1741 ttttccacat cgccaccaga ggcgcccgcc acaccgtcat tcactttgac gtaggtatgc
     1801 acaccggcaa taccggggaa tttcaccgtc agtgtagcaa cgatattttc caccacgcag
     1861 gtttcgcccg tgcagtccac cgcgtcgatg acct
//
LOCUS       DAOQLC010000330         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_43166_length_1888_cov_17.491544, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000330 DAOQLC010000000
VERSION     DAOQLC010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43166_length_1888_cov_17.491544"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PL000_22345"
     CDS             complement(<1..225)
                     /locus_tag="PL000_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91681.1"
                     /translation="MNITKGPMLEEYAKNRDIPIAEVLEWANQIVNQPHLCMAELAKT
                     EKERFADFVAYIKHMNANSSNARIRASIAER"
     gene            complement(222..1163)
                     /locus_tag="PL000_22350"
     CDS             complement(222..1163)
                     /locus_tag="PL000_22350"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC91682.1"
                     /translation="MNALDKIVEMFPGRLDKIDLSAYRDDTYQPRVKCATEYGDALKK
                     RLTQPTIWGAKSGFLCDSYFRFRPREMTIWTGYKGHGKSAVLSQVLLKFMQSGQKVFV
                     CSPEFHPVELMYRFLVQCLGCSTPSEEEADVLLEFFTDTLWVYDVQASLKPNDVAPLC
                     RWVCENIKPDHIVIDSLMKCGISPDDYSAQKTLVDQVQSVAHNNHVHIHLVAHARKGH
                     SDESPARLHDIKGASEIADMAENVISIWRNKPKEKIRDGSKDIEPDAMFIVEAQRNCG
                     GEIGVNPLWFKRDAMIYFEPHKDWRDYVSIGRTEVAI"
     gene            complement(1160..1579)
                     /locus_tag="PL000_22355"
     CDS             complement(1160..1579)
                     /locus_tag="PL000_22355"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNC91683.1"
                     /translation="MSVNNLLNSLDRVRKMGADRWVACCPAHNDKSPSLAIRELSDGA
                     VLLHCFAGCSAHEIVSAVGLDLSELFPEKSDFHVPRQKYPFSAREVLACLVPETFTVA
                     LIGNQMARGIRNDEKTQQALITAVSRISAAHSYVEGL"
     gene            complement(1576..>1888)
                     /locus_tag="PL000_22360"
     CDS             complement(1576..>1888)
                     /locus_tag="PL000_22360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91684.1"
                     /translation="NHKPITNNQTTTPNGFDEFWSAYPKKVGKGAAQAAWKKHKPDLA
                     ICIAAITTAKASRDWQKENGQYIPNPATWINQMRWEDGETVSATPAEFDMQAFLRSKG
                     L"
BASE COUNT          405 a          522 c          479 g          482 t
ORIGIN      
        1 cctttctgcg atggatgcgc gaatcctggc attgctgctg ttcgcgttca tgtgcttgat
       61 gtacgctacg aagtctgcga agcgttcctt ttccgtcttt gctagttctg ccatgcagag
      121 atgcggctga ttcacgatct ggttagccca ctccaagact tcagcaattg gaatgtctcg
      181 gttctttgcg tactcttcca acatcgggcc tttggtgatg ttcatatcgc cacctccgtc
      241 cgtccgatgg aaacgtaatc tctccaatcc ttatgcggct cgaaatagat catcgcgtcc
      301 cgcttgaacc agagaggatt cacgccgatt tctccgccac aattccgctg cgcttcaaca
      361 atgaacatgg catccggctc aatgtctttc gatccgtccc gaatcttctc tttcggcttg
      421 ttacgccaaa tggaaatgac gttttccgcc atgtctgcga tttcagacgc gcccttaatg
      481 tcgtgcagtc gtgctggcga ttcgtctgaa tgccccttgc gggcatgggc aacgagatgg
      541 atatggacgt ggttgttatg ggcaacagac tgaacctgat ccactagcgt tttctgcgcc
      601 gagtagtcgt cagggctgat cccgcacttc atcaagctat caatcacgat atggtcgggc
      661 ttgatgtttt cgcagaccca ccggcacagc ggagctacat cgttcggctt caagctggcc
      721 tgtacgtcat agacccacag cgtatcggtg aagaactcta ggagtacgtc tgcttcttct
      781 tcgctaggcg tcgagcatcc gagacactgc accaggaagc ggtacatcag ctccaccgga
      841 tgaaactcag gagaacatac gaataccttt tgccctgact gcatgaactt tagcaggact
      901 tgagaaagca ccgcagactt gccatgcccc ttgtagcccg tccaaatcgt catttcccgc
      961 ggacggaacc ggaaatagga gtcacacagg aaaccggact tggctcccca tatcgtcggc
     1021 tgagtcagcc gcttcttcag cgcatcgccg tattccgtgg cgcacttgac gcgaggttga
     1081 taggtatcat ctcggtatgc ggaaaggtcg atcttgtcca ggcgaccggg gaacatctcc
     1141 acaatcttgt cgagtgcgtt catagcccct ccacatacga atgcgccgca ctgatacgcg
     1201 aaaccgccgt aatcaacgcc tgttgcgttt tttcatcgtt ccgaatgcca cgggccatct
     1261 gatttccaat taacgcgact gtgaaggttt ctggcaccaa acaagccaat acttcgcgtg
     1321 cgctaaacgg atatttctgg cgcggaacgt gaaaatctga tttctcgggg aacagttccg
     1381 acaggtcgag tccgactgcg ctaacgattt cgtgagcaga acaaccggcg aagcagtgaa
     1441 gcagtacggc accgtccgac agttcgcgaa tcgccaggga aggcgatttg tcgttatggg
     1501 caggacaaca ggcaacccat ctgtctgcgc ccatcttcct aacgcggtcg agtgagttga
     1561 gaaggttatt gacgctcata accctttact ccgcaggaat gcttgcatgt caaactctgc
     1621 cggagtggca gaaacagtct cgccgtcctc ccacctcatc tgatttatcc atgtcgcagg
     1681 gttcgggatg tactgtccgt tttccttctg ccagtcgcgt gaggctttgg cggtcgtaat
     1741 cgcggcaatg cagatcgcaa gatcaggctt gtgctttttc catgcagctt gagcagcacc
     1801 cttgccaacc ttctttggat aggctgacca gaactcatcg aagccgttag gcgtagtagt
     1861 ttggttattg gttattggtt tatggtta
//
LOCUS       DAOQLC010000331         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_43384_length_1883_cov_16.838621, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000331 DAOQLC010000000
VERSION     DAOQLC010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43384_length_1883_cov_16.838621"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..986)
                     /locus_tag="PL000_22365"
     CDS             complement(387..986)
                     /locus_tag="PL000_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91685.1"
                     /translation="MAETDRDLGVAAAAIEAMASTKKLSEFKSFWESFLFRLERAWER
                     NQRRVQTVPGRDAKSWLSTNAKLRRSDPLLNYLKQARNAEVHAISSSIESELIVSVAD
                     RFGRPFSMKDMSLSIEGATFVIDLTSTDIGLEWYGDVALSEPKLQCVINRGKRYDPPT
                     RHLGNAIQNHHPVAISILGLNFYKGTYAALGALLNHKKP"
     gene            complement(1177..1692)
                     /locus_tag="PL000_22370"
     CDS             complement(1177..1692)
                     /locus_tag="PL000_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91686.1"
                     /translation="MIDLEMQLRRADRLDAITKSLGFALWQLQALEETTANYYVLVAL
                     ATRGMGIEAGRKLDDSVKGNTFGKTIHALRDSGKMPSKIEQRFLALLKERNWLVHKSR
                     ATNRGAVHDNQACDSLLVRLDQLAEEARLLLREIGKASEKFVVQSGVPIEEVDRLTHE
                     ILNSWHSTTNE"
BASE COUNT          463 a          466 c          477 g          477 t
ORIGIN      
        1 tcgaaaaatc cggcgccatc cgctacgacc gctgcttcca gtgcctggac tgcgtgggga
       61 tctaccacga ccccgggcgc tgcgcgccgg tgatgctggt gaggaagaag gcggggcggt
      121 aatcaccccg gccaattaat ctcattttct agacagttca tgcctgtcga gagcgctgtc
      181 aaggtggcga agatcgatga ttggtgccaa gccggcgtag aagaggcagg agaacgcacc
      241 gggatcaagg cttcggaatt ctccaagaac caaggaaaat taagtggaat atgggagttc
      301 aggcgacgga agaatgttgc ggcaaacatg atacggcggg aaagacgacg aattcaggaa
      361 cggagattat tgcctagtct gtttggctac ggctttttat gatttagaag cgccccgagc
      421 gcagcatagg ttcctttgta aaaattgagc ccaagaattg aaatcgcaac cggatgatga
      481 ttctgtattg cattaccaag atgccgagtc ggtgggtcat accgctttcc acgatttatg
      541 acgcactgaa gctttggctc tgaaagcgcg acatcaccat accactctaa gcctatgtca
      601 gtgctggtca ggtcgatgac aaaagttgca ccctcaatcg atagactcat gtctttcatg
      661 ctaaatggcc gcccaaatcg atctgcaacc gaaactatca attcggactc aatgctagat
      721 gaaattgcat gaacctcagc gtttctggcc tgtttgagat aatttaacaa gggatcgctt
      781 cggcgcagct tggcatttgt ggatagccat gacttagcgt cgcgacccgg cacggtctgc
      841 acgcgccgtt ggttgcgttc ccatgcacgc tcaaggcgaa agagaaacga ctcccagaat
      901 gatttgaatt cactgagttt ttttgtactt gccattgcct cgatcgctgc ggcagcaacc
      961 ccaaggtctc gatctgtttc agccatttga gatttattga aaactcaaaa tttaaaccgc
     1021 ctcaagaatt tggcaatagt ttttgaatac ggggcttatt gaccgctcgt ggggactgag
     1081 tccccgcaag ccgctgtaac tcaatctcgc cgcaaagaca attagatcgt tttcgcgcag
     1141 tgaggcctag ggatttctgg aacctccatt tcgcgcttac tcattcgtag tggagtgcca
     1201 tgaattcaga atttcgtgcg ttagacgatc aacctcttct attggcacac cactctggac
     1261 aacaaatttt tcagaagcct tgccaatctc tcgaagcaac agtctcgctt cttctgcaag
     1321 ttgatccaat cggacgagaa gtgaatcgca agcttgattg tcgtgcacgg caccgcgatt
     1381 ggttgcacga gatttgtgca ctaaccaatt gcgctcctta agcaaggcta gaaaacgctg
     1441 ttctatctta gagggcattt ttccagaatc acgtaatgcg tgaattgttt ttccgaaagt
     1501 attgcccttt actgagtcat caagcttcct tcctgcctca atgcccatac cacgtgtagc
     1561 tagtgctacc agaacgtagt agttagcagt tgtttcttca agtgcttgaa gttgccacaa
     1621 ggcaaagcct agactcttag tgatggcatc tagacgatca gcccttcgca actgcatttc
     1681 gagatcgatc attagctgac gccggtagtt gtgctgaaga acaggacggg tgtgatgcac
     1741 gagagcggcg cgcgtatcca gaagtcacat attcattatt cttacacaac ggcggcagat
     1801 ttgccgccgt tgcctcgatg cagggctaat gccccccatt aactctcctt ggtcgggggc
     1861 gtgggcttgc tgctgcgctt ggg
//
LOCUS       DAOQLC010000332         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_43650_length_1877_cov_16.113063, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000332 DAOQLC010000000
VERSION     DAOQLC010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43650_length_1877_cov_16.113063"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PL000_22375"
     CDS             complement(<1..353)
                     /locus_tag="PL000_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91687.1"
                     /translation="MKTSAQYQVGIDLGTTNTVVAYAKIGSSDIQLFEIEQLVAAGEV
                     APRPLLPSLRYHATRDELAAADTALPWAANDAGEPAVFGRWARQLGAQVPGRLVTSAK
                     SWLSHSAVDRSAAILP"
     gene            1023..1655
                     /locus_tag="PL000_22380"
     CDS             1023..1655
                     /locus_tag="PL000_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91688.1"
                     /translation="MKKVVLSAALAIVSGAAFAQMTAVEDEVLSAQTGQDGISIALNV
                     KAHIDAFEWIDTDTAAGQGGSVRFENIAIGGGTLSNTAAGTFMGLNGNGAADVIGLAA
                     RDDYSTGIQFGGPINIDVEDVAAFSAGAAAQIGSGTGTDITGAGGYKVHVPAAGTKAV
                     VIGLPRMVMSFNVGAIRVAGGGDTYSMGGIRVTNFDFQSTKVSIWGHKSF"
BASE COUNT          417 a          404 c          528 g          528 t
ORIGIN      
        1 ggcaggatcg ccgcactgcg atccactgcc gagtgcgaca gccagctctt ggcgctggtg
       61 accaaccgcc ccggcacctg cgcgccgagc tgccgcgccc agcggccgaa tacggctggt
      121 tcaccagcat cgtttgccgc ccacggcagt gctgtatcgg ccgccgccag ctcatcgcgc
      181 gtggcgtgat aacgcagtga cggcaacagc ggccgaggtg ccacttcgcc cgctgccacc
      241 agctgttcga tttcaaacaa ctggatatcg ctgctgccga ttttggcgta agcgaccacc
      301 gtgttggtgg tgccgaggtc gatgccgact tggtattgcg cggaggtttt catggggact
      361 gtcgtattgg caacaagtta taactatacc tgagctgcat cagtagccgg ccgtacctgt
      421 agtgacctat ggaaaaattg cgaacgctct ctcgatacca gcttgcaggt tgtccatttg
      481 gcatttctgt acttgttttt gaaaagcggt tgaacagctt cgacgtactg gcacttgaag
      541 tgccgctttg tcagcaagga aaagcggcag gtacttggcg tctactgtac ctaacggagc
      601 cagagtcaag tgggaagccg ccaaggtgct attggcccgg cagcaaaagg ttggtcatca
      661 agcggtagga atgatgccaa aagtggaatg cttgcgactg tatttttgcg tgatcttttc
      721 ttcgctttac ggtgttaatc acatattttt tgttgtggat aagattgttt ttagaatttt
      781 ttcttgtgtg ggttgatgcg ggttatatgg ggtgattttg aatttttata tatataacaa
      841 tgtgttaatt tagtttttat gcaaaataaa aatattcgtt ttgttgttta tgtcaaatga
      901 cttgactgtt gagagttaaa gctctaaagt tgcgtatatg cactattaga catgaaaaag
      961 ctgattcatg cgaagtgcat aacgggaata aataaacttt aatgtcaaac gaggagttaa
     1021 ctatgaagaa ggttgttctg tccgccgctc tggcaatcgt atcgggtgct gctttcgcac
     1081 aaatgactgc agttgaagac gaagtgctga gcgcacagac tggtcaagac ggtatcagca
     1141 ttgcactgaa cgttaaagcc cacatcgacg cattcgagtg gatcgacact gacactgcag
     1201 ctggtcaagg tggttcggtt cgcttcgaaa acatcgcaat cggtggcggc actctgagca
     1261 atactgctgc aggtaccttc atgggtctga acggtaacgg cgctgctgac gtaatcggtc
     1321 tggctgctcg tgatgactac tcgactggta tccagtttgg tggcccgatc aacatcgacg
     1381 tagaagatgt tgctgctttc tcggctggtg cagctgctca gatcggttcg ggtactggta
     1441 ctgacatcac tggcgctggt ggttacaaag tgcatgtacc ggctgctggt accaaggctg
     1501 ttgtaattgg tctgccgcgt atggttatga gcttcaacgt tggtgcaatc cgcgttgctg
     1561 gcggtggcga tacctactcg atgggtggca tccgcgtaac caacttcgac ttccagtcga
     1621 ccaaagtatc gatctggggc cacaagtcct tctaattggg gcttgcttgg atgaaaaagc
     1681 gccacgtata cgtggcgctt tttttatttg tattgcgtgt gtgtgtgggg ggcgtgtttt
     1741 ttgtctaggt atctgttttg atggatttat tgtggtattg gttagtgtgt tttgcataga
     1801 taaatgccga atggttatgt ggatttatgc aaaaggcgtt gacagtgaag agtgaaagct
     1861 ctaaagtaga gttcatg
//
LOCUS       DAOQLC010000333         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_44279_length_1862_cov_13.881018, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000333 DAOQLC010000000
VERSION     DAOQLC010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44279_length_1862_cov_13.881018"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PL000_22385"
     CDS             <1..243
                     /locus_tag="PL000_22385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91689.1"
                     /translation="YNFVMGVLTVFVPAILLWRESRHAGVSALIFFGVHASATVLLLL
                     AFRDVVATQSILAMTFRLAVWLVILALIRASAHKTK"
     gene            262..795
                     /locus_tag="PL000_22390"
     CDS             262..795
                     /locus_tag="PL000_22390"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNC91690.1"
                     /translation="MTKTSPKKYHPIQVTLHWLIVLLVVAAFVMGKSMSGLPNDANKL
                     APLALHMSVGIFTLVVIVTRFITRMKLPKPEHASAGNAFFDWIGKAVHYALYLLVFLT
                     AVSGMSLSMQAGLVPIVFGGSDSPLPADFFDFTARMLHGFIVPALLLLVLLHVGAAFY
                     HQFMLKDNLLARMWYGK"
     gene            843..1265
                     /locus_tag="PL000_22395"
     CDS             843..1265
                     /locus_tag="PL000_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91691.1"
                     /translation="MKTTIGKWNIGVGLWVMASFMIYGFVLIYLRDFAPGKEAWIESY
                     NTGAHFEARLAHVHGNLFSLLNIVFGFLLMKLQLKDNLARWASWLALAGLLMPLGILG
                     EVYLGLPYYFVLVGGISIFLATVLLGVAVVQMKNEIKG"
     gene            1268..>1862
                     /locus_tag="PL000_22400"
     CDS             1268..>1862
                     /locus_tag="PL000_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91692.1"
                     /translation="MPIPYLLTTLLYTFVAMFVAADASLVSMTVFSAFPALRWVRVHF
                     ITLGILSQLVFGVLPILTASLSKKEKPAMRWDIWLMLNAGLVTLVAGFASMNHPMIFT
                     GGTLIFIAALLLILQLWNIRGGDAPASLKFYITGIFYLLVGIIIGTGLFLNWSDALYI
                     KVPLEAHIHANSWGFMSLVFAGLMVDVIPMVTGKPLAS"
BASE COUNT          375 a          437 c          489 g          561 t
ORIGIN      
        1 tataactttg tgatgggcgt gctgacagtt tttgttccag cgattttgct ttggagggag
       61 agccgccatg cgggggtttc tgcgctgatc tttttcggag ttcatgcgag tgcaaccgtt
      121 ttgttgttgc tggcatttcg tgatgttgtg gcaactcaaa gcattttagc gatgactttt
      181 cggctcgcgg tctggctggt cattcttgcg ttgattcgtg cttcggcgca taagacaaaa
      241 taagttttca aggagaacaa catgacaaaa acatccccta aaaaatatca tcccatacaa
      301 gtgacactgc actggctgat tgtgcttctg gtagttgccg cgtttgtgat gggcaaatcc
      361 atgagcgggc tgccgaacga tgcgaacaaa cttgcgcctt tggcattgca catgagcgtt
      421 gggattttta cactggttgt gatcgtgact cgtttcatca cacgcatgaa actccccaaa
      481 cctgaacatg cctcagcggg taatgctttc ttcgattgga tcggtaaagc ggtgcattac
      541 gccttgtatc tgctggtctt tctgacggcg gtcagcggca tgtcgctttc gatgcaggct
      601 gggctggtgc cgattgtctt cggcggttca gattccccgc tacccgcaga cttcttcgat
      661 ttcacggcgc ggatgctgca tggcttcatt gtgcctgctc tgcttttact ggttctgctg
      721 catgtcggcg cggcgttcta tcatcaattc atgctcaagg ataatcttct tgcgcgtatg
      781 tggtatggaa aatagatcac agtgtaaggc gacattaatg tcgccttaca aaaggagaaa
      841 acatgaaaac aactattggt aaatggaata tcggcgtggg actgtgggtg atggcgtcct
      901 tcatgatcta tggctttgtc ttgatctacc tgcgggattt tgcgcccggc aaggaagcct
      961 ggatcgaatc ctacaacacg ggcgcacatt ttgaagcgcg gctcgcgcat gtgcatggca
     1021 atctgttttc actgcttaac atcgtcttcg gctttttgtt gatgaaactg caattgaaag
     1081 acaatcttgc caggtgggct tcgtggctgg cgttggcagg cttgcttatg ccgttgggaa
     1141 ttttgggtga agtatatttg ggcttgcctt attactttgt gttggtcggc ggtatttcga
     1201 tattccttgc cacggtgttg ctgggtgtgg cagttgtaca aatgaaaaac gaaatcaaag
     1261 gataaaaatg ccaatcccat atttacttac tactttgctt tacacgttcg ttgccatgtt
     1321 cgtggcagca gatgcttcac tcgtcagcat gaccgttttt agcgcctttc ctgccttgcg
     1381 ctgggtccgt gttcacttca tcacattggg catcctttcg cagctggtct ttggcgtact
     1441 gcccattctt actgcttcgc tttccaaaaa agagaagccc gccatgcgtt gggatatttg
     1501 gttgatgctc aatgcgggtt tggtgactct ggtggcagga ttcgccagca tgaaccaccc
     1561 gatgatcttc acaggcggta cactgatctt catcgcagca ttgcttctaa ttttgcaact
     1621 ctggaacatt cgcggcggtg acgcgcctgc cagcctcaag ttttacatca cgggcatttt
     1681 ctatttgctg gttggcatca tcatcggtac gggcttgttc ctcaattgga gtgacgcgct
     1741 ctacatcaag gttccgcttg aggcacacat ccacgccaac tcgtggggct ttatgtcgct
     1801 ggtctttgcc ggtctaatgg tggacgtcat accgatggtc acggggaaac cgctcgcttc
     1861 ta
//
LOCUS       DAOQLC010000334         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_45877_length_1828_cov_12.754653, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000334 DAOQLC010000000
VERSION     DAOQLC010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45877_length_1828_cov_12.754653"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PL000_22405"
     CDS             complement(<1..770)
                     /locus_tag="PL000_22405"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNC91693.1"
                     /translation="MFEEVTIGECRLILGDCREVLPLLPPVDLVLTDPPYGIGADARM
                     HAAAGKKSSGGAAFKKDYGATNWDSETPPDWLFGLMMSKSENQIIFGGNYFNLPPTMC
                     VLVWDKDNGTTKFSDFELAWTSLQQAARLKRYMWNGMLQQDMKNKEEREHPTQKPVPV
                     MRWALMQAKGVQTVCDPFMGSGTTGVACVMEGLQFVGIERERKYFDIACRRIEQAYAQ
                     PRLFEDAKVGAGDTAVQVDLLPANAEVRGDAPLYGAASL"
     gene            complement(869..1519)
                     /locus_tag="PL000_22410"
     CDS             complement(869..1519)
                     /locus_tag="PL000_22410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC91694.1"
                     /translation="MAEKVVIGNAELWHGDCMEILPTLPEFDLICTDPPYGIGAGSGT
                     GGNNAAKAKDYGPDTWDKETPPIWLLELILVKGKHQIIFGGNYFRMPPTSCVLVWDKD
                     NSGNFADAELAWTNLKKAVRLKKWRWNGMLQENMAKKEEREHPTQKPVPVMEWAITQA
                     GEDVVTVCDPFMGSGTTGVACANLGKRFVGIERERKYFDIACERIRAAQAQERLFA"
BASE COUNT          356 a          610 c          414 g          448 t
ORIGIN      
        1 agggacgccg cgccataaag cggcgcgtcg cccctcactt ctgcgttggc aggcaaaaga
       61 tccacctgca ccgccgtatc gccagcgccg actttcgcat cctcgaacaa ccggggctgt
      121 gcgtaggctt gctctattcg gcggcaggcg atgtcaaagt atttgcgctc gcgctcaatt
      181 ccgacgaact gcaaaccctc cattacgcaa gcgactccag ttgtcccaga ccccataaat
      241 ggatcgcaaa ccgtctgaac ccccttggcc tgcatcagcg cccaccgcat caccggcaca
      301 ggcttttgtg ttgggtgctc gcgttcttcc ttgttcttca tgtcttgctg aagcatccca
      361 ttccacatat accgcttcaa tcgtgcggct tgctgaaggc tcgtccacgc aagttcaaaa
      421 tcagaaaact ttgttgtccc gttgtcttta tcccaaacca gaacgcacat cgttggtggt
      481 aggttgaaat aattgccacc gaaaattatc tggttttcgc tcttgctcat catcaaacca
      541 aacaaccaat ccggcggggt ttcgctgtcc caatttgtcg ccccgtagtc ttttttaaaa
      601 gccgcgcctc cgctgctttt ctttcctgct gctgcgtgca ttcgagcgtc cgcgccaatc
      661 ccatacggcg ggtcggtcag taccagatcg acgggcggca gcagcggcag cacctctcgg
      721 cagtcgccga ggatcaacct gcattcaccg atggtcactt cttcaaacat ggtcactccg
      781 ttttacgttt tgcctgccaa cccgtcgctc aagcgggacg cgccgcgata aagccgcgtc
      841 gcgcccctta gctatgcgtt agcgggcttc atgcgaacag ccgttcctgc gcctgggctg
      901 cccgtatccg ctcacacgca atgtcaaaat acttcctctc tcgctcaatc cctacaaacc
      961 gcttgccgag gttcgcgcag gctactccag tggttccgct gcccatgaag gggtcgcaaa
     1021 ccgtcacaac atcttcccct gcttgcgtta tcgcccattc catcaccggc accggctttt
     1081 gtgtcgggtg ttcgcgttcc tctttctttg ccatattctc ctgcaacatc ccattccatc
     1141 gccacttttt caggcgtacc gcttttttta aattcgtcca cgccagttcc gcgtctgcaa
     1201 aattcccgga gttgtccttg tcccaaacca acacacaact tgtcggcggc atcctgaagt
     1261 agttgccgcc gaaaataatc tggtgtttcc ccttcaccaa aatcaactcc aaaagccaaa
     1321 ttggcggcgt ttccttgtcc caagtgtcgg ggccatagtc ctttgctttt gcggcgttgt
     1381 tcccgcctgt tccactgcca gcgccaattc catacggcgg gtcagtgcaa atcaagtcaa
     1441 actctggcag cgtgggcaat atctccatgc agtcgccgtg ccacagttca gcgtttccaa
     1501 tcactacttt ttcagccata gttcctcact tcgatcaaac agcccgctaa cacgtcattc
     1561 cagcggacgg gcaaaaagcg cccgccgctg aattcaggcg ttaggttcct tggcgcggtc
     1621 tatccgatcc cgcatccact ttgccccacc caggcgcttc agcttctcgc gttgcgccgc
     1681 tgtcatgcgg agagagaccg ttaaggtctc ctcccctgct gccagaggct tgcgcccctg
     1741 gccccttccg ggaccgccac ggttattcat tccgtccata tccagtaacc atgctccagg
     1801 caccaccaga aaccgtatga ggagcact
//
LOCUS       DAOQLC010000335         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_45920_length_1827_cov_14.142777, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000335 DAOQLC010000000
VERSION     DAOQLC010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45920_length_1827_cov_14.142777"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PL000_22415"
     CDS             complement(<1..636)
                     /locus_tag="PL000_22415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNC91695.1"
                     /translation="MEQYLQKDYTGGAFELYGPAHLVALGVIVIACFVLIRFGKSWNE
                     QQRRNFRYFMAVFLIVDELSWHAWAAYWGIWTVQTMLPLHICSVMVWASAYMLFTKNY
                     NIYEMAYFLGIGGATQALLTPDANQYGFPHYRAFQTFIAHGLLVIIPIYMTLVEGYRP
                     TLQSFKRVFIWTNIYMIIVFFINLAIGSNYLFIAHKPEFPTLLDMLAPWPWY"
     gene            complement(696..1433)
                     /locus_tag="PL000_22420"
     CDS             complement(696..1433)
                     /locus_tag="PL000_22420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HNC91696.1"
                     /translation="MGQYFSLVYEGEPISLFGRGHLIALTIIVLFCFSFLYFRKVWGE
                     KEKKIARWVLAILIVVNESALHIWSAYWGIWNIQTMLPLHMCSVMIWASAYMLVTRNY
                     SIYEMVYFLGLGGAMQAVLTPADAAAYNFPHFRIMQTFTAHGLLIGIAIYMTVVEGFR
                     PTLQSFKRVFIWTNLYMIVIFFLNLAIGSNYLFVAGKPEFPTLLDMLAPWPWYIIELE
                     AIGFAIFFLLYVPFLIKDWKAKQATTA"
     gene            1635..>1827
                     /locus_tag="PL000_22425"
     CDS             1635..>1827
                     /locus_tag="PL000_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91697.1"
                     /translation="MKQRNLYIVLAVIVLAVVAVVYFSMNSKPAAPAQLTEEVKLTEM
                     PVTEIAMSGPASTPEAEFSG"
BASE COUNT          517 a          439 c          444 g          427 t
ORIGIN      
        1 gtaccagggc cagggggcca gcatatccaa cagggtgggg aattcgggtt tgtgcgcgat
       61 gaagagatag ttgctgccga tggctaggtt gataaaaaag acgatgatca tgtagatatt
      121 tgtccaaatg aagactcgtt tgaacgattg cagagtgggg cgataccctt caaccaaggt
      181 catgtaaatg gggatgatca cgagcagtcc gtgtgcgatg aaggtttgaa aggcacggta
      241 atgtgggaaa ccatattgat ttgcatctgg agtgagaagg gcctgcgttg ctccgccgat
      301 gccaagaaaa taagccattt cgtaaatgtt gtagtttttg gtgaaaagca tgtaagccga
      361 ggcccacacc atcacactgc aaatatgcag aggcagcatg gtttgaacgg tccaaatgcc
      421 ccaatatgcc gcccatgcgt gccaacttaa ttcatccacg atcaggaaga ccgccatgaa
      481 atagcggaag tttcgtcttt gctgttcatt ccagcttttt ccaaaacgga tcaagacaaa
      541 acaggcgatg acgatgacgc ccagcgcaac cagatgggct gggccatata actcgaaggc
      601 gccgcctgtg taatcttttt gtaaatattg ctccatgctc agctcctttt tgtgaattgc
      661 tgttaaaacg cttttgcatg gcgcgagttt cggttttagg cagtggtcgc ctgtttggcc
      721 ttccaatcct tgatgagaaa cggcacatac aacaggaaga agatcgcaaa cccgatggct
      781 tccagttcga tgatgtacca gggccagggg gccagcatat ccagcagggt ggggaattca
      841 ggcttgcccg ccacaaaaag ataattactg ccaatggcaa gattcaggaa gaaaatgacg
      901 atcatgtaga gattcgtcca aatgaaaacc cgtttgaaag attgcagggt gggtcggaat
      961 ccctccacca cggtcatgta aatggcgatt ccgatcaaca atccatgcgc ggtgaaagtc
     1021 tgcatgatcc gaaagtgtgg aaagttgtac gcggcggcat cggctggggt caataccgcc
     1081 tgcatggctc caccaagccc aagaaaatac accatttcgt agatgctgta atttctcgtc
     1141 accagcatgt aagccgaggc ccagatcatg acactgcaca tgtgcagcgg cagcatggtt
     1201 tgaatattcc atatccccca atatgccgac cagatatgca aggccgactc attgaccacg
     1261 atcaatattg ccagaaccca gcgggcaatc ttcttttcct tttcccccca cactttacgg
     1321 aaatacaaaa atgaaaaaca aaatagaaca atgatcgtca gggcgatcag gtggccccgc
     1381 ccgaaaagtg agatcggctc cccttcataa accagtgaaa aatactgtcc catatcaact
     1441 ccctttctcg cagttcatct gccttgcgta tttttaccac aggatataaa cttcgacgcc
     1501 cgtaaattaa agcatcttta aagcggcgtt acaaaagacg ggatgcgaat ataatcttac
     1561 gaaacacaaa ctggttgccg ccttgcgcaa agaaccacag gcggccaaat ctaaaacatg
     1621 ttggaggaat tgttatgaaa caacgaaatc tgtatattgt attggctgtc atcgtactgg
     1681 cggttgtcgc agtggtttat ttttccatga attcaaaacc tgccgcacct gcccagttga
     1741 ccgaagaagt caaactcact gaaatgcctg ttaccgagat cgccatgtcc ggacctgcat
     1801 ccacacctga agcggaattc tccgggc
//
LOCUS       DAOQLC010000336         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_46302_length_1818_cov_33.153148, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000336 DAOQLC010000000
VERSION     DAOQLC010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46302_length_1818_cov_33.153148"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1579)
                     /locus_tag="PL000_22430"
     CDS             complement(122..1579)
                     /locus_tag="PL000_22430"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HNC91698.1"
                     /translation="MSTESKAKRVERQARLTLKNCKAQKIDDGGGFLTALGGVTSLAK
                     AAEVTGLVAMAVERIEPWRDEGLVEHEIKTLLSQRLYLGACGHTDALDCSLFKDDPGL
                     KSVLGLEPDGPSLPSQSTHTRMENSMSEDSLNKLEELPLEYFLSRHEHEPRELTIYFD
                     GSAIRTYGSQQNSIYRGGKKYSQTQYFPLLAATKEGHLLLAKLRSGNVSDATSLAEIT
                     KLVEAILERWPHVKLTIVMDAGFNEPHLLDFLEEKKVNYVVGYPATSSIKSNFKDTIK
                     AAEEGFKKEFGEPKFKGKAGEKAWQKEHTRLRALPADERKEAEKAMNSRFTRWMYGAE
                     HQGNNWEGSRTVIVRIDCSDKGTDIRCVSTNMTGHSAQAIYEDYYCNRATIEFTIKEN
                     KSHCRVPLSCMEYSANRFRFVLQSLIYILMDELRRLLPSSKTALTLATLRNQILLIPA
                     QIFAYARHIHWRLSSVHPASALLINLNKKLQARTA"
BASE COUNT          424 a          488 c          406 g          500 t
ORIGIN      
        1 gggttttctg aaaagcagct aaaaaatcca gattaaacgg cagcgaaagg ggctctgcgg
       61 agatgccccg gctcaagtag gcgagccagg ttctttgttt gtcgcgtttc tggttgaagc
      121 ttcaggctgt tcgggcttgc agcttcttgt tcaggttgat gagtagagca gatgccgggt
      181 gtactgaaga taggcgccag tgaatatgcc gcgcataggc gaaaatttga gcgggaatca
      241 gaagtatttg attgcgcaag gtggctaagg tcagggcagt tttggaactc ggcagcaagc
      301 gccttaattc atccatgagt atgtagatta agctctggag aacgaacctg aacctattgg
      361 cgctgtattc catgcagctc aatggcacac ggcagtgact tttgttttcc ttgatcgtga
      421 attcgatagt ggctcggttg cagtagtaat cctcgtaaat cgcctgggcg gaatgaccgg
      481 tcatgttagt ggagacacac cgaatgtcag tgcctttgtc gctgcagtca attctaacta
      541 ttaccgtgcg gctcccctcc caattgttgc cctggtgctc ggctccgtac atccagcgcg
      601 tgaacctgga gttcattgcc ttctccgcct cttttcgctc atcggccggc agtgcgcgca
      661 acctagtatg ctccttctgc catgccttct cgccagcttt acctttgaat tttggctcac
      721 caaactcttt cttgaagcct tcttctgcgg ctttaatcgt atctttgaag ttagacttga
      781 ttgacgatgt tgcaggatat ccaaccacgt agttgacctt cttttcctcc aagaagtcca
      841 gaagatgggg ctcattaaac ccagcatcca taacaattgt aagtttcaca tgcggccaac
      901 gctccaaaat tgcctccacc agttttgtaa tttccgccag cgatgttgca tcggaaacgt
      961 tgcctgaccg tagctttgca agcagcagat gaccttcttt tgttgctgcc aacagtggga
     1021 aatactgtgt ctgactgtat ttcttcccgc cgcgataaat agaattttgc tgcgagccgt
     1081 aggtcctaat cgccgaacca tcaaaatata tggtcagctc cctcggctca tgctcgtgcc
     1141 tggaaagaaa atactccaga ggaagctcct ccaacttgtt gagcgagtct tctgacatcg
     1201 aattttccat gcgcgtatgc gtggactgcg acggcaacga cggcccatcc ggctcaagtc
     1261 ctaaaacact cttcagccct ggatcatcct taaacagaga acaatctagc gcatcagtat
     1321 gcccacacgc cccaagatac aatcgctggc tcaaaagagt cttgatttca tgctcgacta
     1381 aaccctcatc ccgccacggc tctattctct ccacagccat tgctacaagt cctgttacct
     1441 ctgctgcctt cgccagggac gtaacgcctc ctaaagcagt caaaaagccc ccaccatcat
     1501 caatcttttg tgctttgcag ttttttaacg tcaaccgggc ttgccgttcc actcgctttg
     1561 ccttagactc tgtgctcatg aaagttcctt ttgccttgct gctttttgtt tttcgcaact
     1621 caattatcca gcaaccatca ggactttcat ccttttaaac tacactctga taccatccgc
     1681 aatactagat ctcgattagc aatacaatcg gagcctggct gatatagccg ctttgtggca
     1741 gataatcaac aaccgcaaaa gtgttagcgt gaaaactccc ggcaaagatt aaacgcgctc
     1801 tccgtctggt atctactc
//
LOCUS       DAOQLC010000337         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_46419_length_1816_cov_13.398637, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000337 DAOQLC010000000
VERSION     DAOQLC010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46419_length_1816_cov_13.398637"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..269)
                     /locus_tag="PL000_22435"
     CDS             complement(36..269)
                     /locus_tag="PL000_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91699.1"
                     /translation="MPHTEKLPSAWDLLTKIFDPMTPLDAAQVGALMGRRDLDVEYVG
                     DDEPGFIPYEPSSLGRVFRLGDVVLALAPILFR"
     gene            complement(288..806)
                     /locus_tag="PL000_22440"
     CDS             complement(288..806)
                     /locus_tag="PL000_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91700.1"
                     /translation="MKKTTDRILTAIFCDDIRHEVGNKMSFMGSYQGELFVPAVPTGL
                     SKLCIFATALTPIGRPFKSLTFRIVMDDNVDLARLEIPTEAFPAIPDVVDETATRKSV
                     STALMFSPFIIEKPTSLRLMADTEEGEIVGPRLLIKVAPEMLQTMVASPAEQAPIVVE
                     KKTRRKRTVPAK"
     gene            complement(803..1231)
                     /locus_tag="PL000_22445"
     CDS             complement(803..1231)
                     /locus_tag="PL000_22445"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC91701.1"
                     /translation="MDSPSISLVNAVPQTSTAKGQVIYKEFSAPPKPLAMPGAIDIDD
                     LVAELESQSPENAEAIAHGRKWVAETFYADRPSVAQLRLKKGWSQAELARRAETSQSY
                     IARLEQGKIDPQISTARKIAAVLGVSIEILADALGSENVA"
     gene            complement(1212..1700)
                     /locus_tag="PL000_22450"
     CDS             complement(1212..1700)
                     /locus_tag="PL000_22450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91702.1"
                     /translation="MQDLTPMVCDPNGVLNHFLTSNTRLGIVSSLSIHPDAAADLRRL
                     METDRFAAGKVAALLQQAKHDPKIIATLLDHDFGDDHSAAYHVSKWFEFWNAGYNLWR
                     LKIWIEPSGSLRYRIVYAYEPRTLQYHVLAIVHRDFDYKKDHDITRRIRKAYNDLGLT
                     IH"
BASE COUNT          442 a          476 c          459 g          439 t
ORIGIN      
        1 ggtcaggaca gagccgcatt ctgacatatc tcaatctatc tgaacagtat tggggcaagg
       61 gccagcacca catcgcccaa gcgaaatacc cggccaagcg atgatggttc ataaggaata
      121 aagccgggtt cgtcatcccc gacgtattcc acatcgagat cacgccgccc catcaatgcg
      181 ccgacctggg cggcatcaag cggtgtcatg gggtcgaaaa tcttggtgag caggtcccaa
      241 gcggatggca gtttttcagt atgcggcact tacaaccttt cctcgcctta ctttgctggc
      301 acagtcctct ttcgccgagt ttttttctcg acaacgatgg gggcttgctc agccggggat
      361 gcaaccatcg tttgtagcat ttctggtgct accttaatga gcagacgagg gccaacgatt
      421 tcgccttctt ccgtatccgc catcagccgc aatgacgtag gtttctcaat gatgaaggga
      481 gaaaacatga gtgcggtgct aacagactta cgggttgctg tttcatcaac aacatcaggt
      541 atggcaggaa acgcctccgt tgggatttca aggcgcgcca gatctacgtt gtcgtccatg
      601 acaatccgaa acgtcaggct tttgaatggt cttccaatcg gagtcaaagc cgtggcaaaa
      661 atgcacagtt ttgaaaggcc tgtcggcaca gcaggaacga acaactcgcc ttgataactc
      721 cccatgaacg acattttgtt gcctacttca tgtcggatgt catcacaaaa aatcgccgtc
      781 aagatgcggt ctgtcgtctt tttcatgcca cattctccga accaagcgca tcggccagaa
      841 tctcaatcga aacccccaag acggctgcga tttttcgcgc cgtactgatt tgcgggtcaa
      901 tctttccctg ttccagccgg gcaatatagg actgactggt ttctgcacgc cttgccagtt
      961 cggcttgcga ccaacccttt ttcagccgga gttgcgcaac actaggccgg tcggcgtaga
     1021 aagtttcggc aacccatttc cgaccgtggg caattgcctc ggcattttcc ggcgactggc
     1081 tttccaactc ggcaaccaag tcgtcaatgt cgatagcgcc gggcatagcc aacggcttgg
     1141 gtggtgcgga aaactccttg taaatcacct gccccttggc ggtgctggtc tggggtacgg
     1201 cattgaccag actaatggat ggtgagtcca aggtcgttgt aggctttacg gatacggcga
     1261 gtgatgtcat ggtctttttt atagtcgaag tcgcgatgaa cgatggctaa tacatggtat
     1321 tgaagggtac gcggctcata ggcatatacg atgcgatacc gcaggctgcc cgatggttca
     1381 atccatattt ttaaccgcca taggttgtat cccgcattcc agaactcaaa ccacttgctg
     1441 acgtgataag ccgccgagtg gtcatcccca aaatcatggt caagcaatgt ggcaatgatt
     1501 ttggggtcat gcttcgcttg ttggagcaaa gcagcaacct tcccagcagc aaatcggtca
     1561 gtttccatca accggcgcaa atccgccgca gcatcaggat gaatcgacag ggatgaaacg
     1621 atacccaatc tggtattact cgtcaagaaa tggttgagca caccattggg gtcacacacc
     1681 attggggtca ggtcttgcat ttaatcattt ccagaaacca ttgaagtcag atcccgtaat
     1741 actccaagtt ttctagggcg tgttcacagt agccagacat aagcgcaagc cagatgaaga
     1801 aaggcattga atcgat
//
LOCUS       DAOQLC010000338         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_46540_length_1814_cov_20.933485, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000338 DAOQLC010000000
VERSION     DAOQLC010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46540_length_1814_cov_20.933485"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PL000_22455"
     CDS             complement(<1..181)
                     /locus_tag="PL000_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91703.1"
                     /translation="MISRLKIAGVLAFVIGALSIFAGGMAIRGWNPGYSVLGWLPVYN
                     FVMGVLTVFVPAILLW"
     gene            complement(178..498)
                     /locus_tag="PL000_22460"
     CDS             complement(178..498)
                     /locus_tag="PL000_22460"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNC91704.1"
                     /translation="MSETSQIYFAETKSKAVFAADGPKPQFLIDTPKFKSLVVGLEAG
                     GQIPVHPGEAAMYHFLEGEGLMMVDDETFAIKPGVTVVVPSGAKRGMNAKTRVVFLGA
                     KGEA"
     gene            complement(580..1266)
                     /locus_tag="PL000_22465"
     CDS             complement(580..1266)
                     /locus_tag="PL000_22465"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNC91705.1"
                     /translation="MTDPSLLLKRLQEFDFLRGLDHETFTTLANSAVWKVFPPNAVIF
                     WEGDTESNLYYLQYGSLKALRTAPDGREQVLRFLDAGEIFNEIGVLAKRPNPVTAVAL
                     EESGIWLIPRHALEEVVMKYPQTALQIIGNMADKIISLVTLAADLSLKTVEARFAKLL
                     LDSAEGDVIERRRWTNQTELAAHLGTVPDVLSRVIRELTKAGLIEMDKQQIRILNRAG
                     LAERAMIRGD"
     gene            1459..>1814
                     /locus_tag="PL000_22470"
     CDS             1459..>1814
                     /locus_tag="PL000_22470"
                     /inference="COORDINATES: protein motif:HMM:NF021485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2202 domain-containing protein"
                     /protein_id="HNC91706.1"
                     /translation="MFNNFKHILIGVLSAVVVIAVGASAYNAFASPSAPTANTADLAQ
                     YGNGNGNGGNGNGGQTGGGGVQLTAIPASDLSAEEAAALLFMREEEKLARDVYNQMYA
                     LWGQPVFQNIAASEQT"
BASE COUNT          440 a          492 c          453 g          429 t
ORIGIN      
        1 tccaaagcaa aatcgctgga acaaaaactg tcagcacgcc catcacaaag ttatagacag
       61 gcagccagcc cagtacagaa tagcccggat tccatccgcg gatggccatg ccgccagcaa
      121 agatcgagag ggcaccgatc acaaacgcaa gtacgcccgc gattttcaac ctggagatca
      181 cgcttcacct tttgcgccta gaaacaccac gcgcgtcttc gcattcatgc cgcgtttcgc
      241 gccgcttggc acaaccaccg tcaccccggg tttgatggca aacgtttcat cgtccaccat
      301 catcaagcct tcgccttcga gaaagtgata catcgccgct tcgccagggt gaacgggaat
      361 ttgtccgcca gcctccagcc ccaccaccag tgacttgaac ttcggcgtgt cgatcaaaaa
      421 ctgcggcttg ggtccatcag ccgcaaagac cgctttggat tttgtttctg caaaatagat
      481 ttgacttgtc tcagacatat cgtctccttt gatatgcctg actttaatgg aagtccccgt
      541 ttcacacccc gacttttgtc gggtttttca gcccaaactt taatctcccc gaatcatggc
      601 tcgctccgcc aagcctgcgc ggttgaggat gcgaatttgc tgtttgtcca tttcgatcag
      661 cccagccttg gtcaactcgc ggatgacgcg actgagcaca tcgggcacgg ttcccagatg
      721 cgcggcgagc tcggtttgat tcgtccagcg gcggcgctcg atcacatcgc cttcggcaga
      781 gtctaaaagg agtttggcaa agcgtgcctc aaccgtcttg agtgacaggt ccgctgccaa
      841 tgtgaccagg ctgatgatct tgtctgccat gttgccgatg atttgcaatg ccgtctgcgg
      901 atatttcatc accacctctt ccaacgcgtg gcgcggaatg agccaaatgc cagactcttc
      961 caatgccacc gccgttacag ggttgggacg tttcgccagc acaccgatct cattgaagat
     1021 ctcgcctgcg tccagaaaac gcaatacctg ttcgcgtcca tcgggtgcgg ttcgcagggc
     1081 tttcaatgaa ccatattgca gatagtacaa atttgattca gtatcgcctt cccaaaaaat
     1141 cacggcattg ggtggaaata ctttccacac ggcgctgttt gccagggtcg taaaagtttc
     1201 atgatctaac ccgcgcagga agtcaaactc ttgcaggcgt ttgaggagca gggatgggtc
     1261 tgtcatggcg cgaagtgtag catgaaaaag ttcaggtcag cgtttgaggc gggcgatgga
     1321 tttgttatta ataacaagaa cattttccaa gtcttgaaag gatgtttaca aagcctttac
     1381 atgaacgttc tatcatgagg tcatcgtaaa aaagaaacgc cgcgtttcaa gcgcgacgtt
     1441 caaacaagga gaaaaatcat gttcaacaat ttcaaacaca ttctcattgg cgtcctttca
     1501 gcggtggtag tgatcgccgt gggcgcgagc gcctataatg cttttgcttc tccatcggct
     1561 ccaacagcca atactgcaga ccttgcgcaa tatggcaatg gaaacggtaa cggcggcaat
     1621 ggtaatggcg gacagacagg tggaggtggt gtgcagttga cagccatccc tgcttcagat
     1681 ttgagcgcag aagaagctgc tgcactcttg ttcatgcgcg aagaagaaaa gcttgcgcgc
     1741 gatgtgtaca accagatgta tgctctttgg ggacagcccg tctttcagaa tatcgctgcc
     1801 agcgagcaga ctca
//
LOCUS       DAOQLC010000339         1797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_47401_length_1797_cov_13.114811, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000339 DAOQLC010000000
VERSION     DAOQLC010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47401_length_1797_cov_13.114811"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1572
                     /locus_tag="PL000_22475"
     CDS             76..1572
                     /locus_tag="PL000_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HNC91707.1"
                     /translation="MSLARMAAPAPTLLYERHRPEETLLYQLIETHYPAFRVQLEAQG
                     QTLPAFVKQEFDDFLTCGRLDYGFLRVCCEDCKHERLVAFSCKRRGFCPSCGARRMAE
                     CAALLVDEVLPQEPIRQWVLSFPYELRFLLAQQPAMMGKVLEIVHRTLATHLVKKAGF
                     NKRTAHTGAVTLIQRFGSALNLNIHFHMLFLDGVYAEDKYGVMRFHRVKAPTIEELNA
                     LVHQISHRVTRILEKKGWLQRDDENTYLLLDSIDQEDDALRQLQGHSITYRIAMGPQK
                     GRKVLTLQSLPPRLDALRESDRVAKQAGFSLHAGVMAEPHQRDVLERLCRYISRPAVS
                     EKRLALTNNGKVRYELKTPYRDGTTHVFFDPLDFIARLAALVPKPRVNLTRFHGVFAP
                     NNKHRALVTPANRGKGKKTAEEGCVQEKTIAERRKAMTWMQRLKRVFNIDIEKCERCG
                     GHVKVVSCIEDPDVIEKILQHLALKESPPLHRVHEARAPPDQAALFPI"
BASE COUNT          436 a          521 c          459 g          381 t
ORIGIN      
        1 gccccgttcc gcccctgtcc aagaccctga gcaattgtta agctgtatat atgtacagct
       61 tcaatggatt gaatcatgtc attggccagg atggccgcgc ctgctccgac gctcctctac
      121 gagcgccacc ggccggaaga aacgctgctc tatcaactca tcgaaaccca ttaccccgcg
      181 tttcgcgtcc aacttgaagc tcaaggacaa accctgcctg ccttcgtcaa gcaggaattc
      241 gacgacttcc tcacatgcgg tcgtctcgac tacggattct tgcgcgtgtg ctgtgaagat
      301 tgcaagcatg agcgcctcgt cgctttcagt tgcaaacgga ggggattttg ccccagttgc
      361 ggcgctcggc gaatggcgga gtgtgccgcc ttgttggtgg atgaagtgtt accacaagaa
      421 ccgattcgcc agtgggtcct aagctttccc tatgagctgc gttttctgct ggcccaacaa
      481 ccggccatga tgggtaaggt gctggagatc gttcatcgca cgttggccac acacttggtc
      541 aagaaagcag gttttaacaa acgtaccgct cacaccggcg ccgtcaccct gatccagcgc
      601 tttggctctg cgctaaacct gaatattcac tttcacatgc tgtttctaga cggcgtttac
      661 gctgaagaca aatatggcgt catgcgtttt caccgcgtca aggcgccgac gatagaagaa
      721 ctcaatgccc tcgttcacca aatcagccat cgcgttaccc ggattctgga gaaaaaaggc
      781 tggctgcaac gtgacgatga aaacacttac ctgctactgg attccattga ccaagaggat
      841 gacgccctcc gccagttgca aggacactcc atcacctacc gcatagccat ggggccgcag
      901 aaaggacgca aggtgctcac cctgcaaagc ctgccgcccc gcttggatgc cctcagagaa
      961 tcagaccgtg ttgccaaaca agcgggattt tccttgcacg ccggagtaat ggccgaaccc
     1021 caccagcgcg acgtgctgga aaggctgtgc cgctacattt cccggcctgc ggtatcggaa
     1081 aagcggctgg cgctcaccaa caacggtaaa gttcgctatg aactgaagac gccgtatcgt
     1141 gatggcacca ctcatgtctt tttcgatcct ctggatttca ttgcgcgatt ggccgcgctt
     1201 gtaccgaaac cgcgtgtgaa tctgacacgg tttcatggag tgttcgcccc caacaacaag
     1261 catcgggcac tggtcacgcc agcaaacaga ggcaaaggca aaaaaacggc agaagaggga
     1321 tgcgtgcaag aaaagaccat tgccgaacgc cgcaaggcca tgacctggat gcaacggctc
     1381 aagcgtgtct tcaacatcga tattgaaaag tgcgaacgct gcggcgggca tgtcaaggtc
     1441 gtctcctgca tcgaagaccc cgacgtgatc gaaaaaattc tacaacatct ggcgctgaag
     1501 gaatcgccac ccttacaccg ggtccatgaa gcgagggcgc cgccggatca ggcggcgttg
     1561 ttcccgatct aaaccaccaa tctatttcct tcaccaacaa cttgccccaa cacggcagga
     1621 tgagacacgt ctgttgggtg catctttcaa gaaaaccgct gaaaaaccga ccgccagtgc
     1681 ctacgctgcc gaactgaaaa ggctggattc tggcgtcgaa aggatcattg cgcagttcat
     1741 gcacggattt catatgatag gatttcagtg aaaaggcgct ttattcgtcc tataccc
//
LOCUS       DAOQLC010000340         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_47846_length_1788_cov_17.572418, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000340 DAOQLC010000000
VERSION     DAOQLC010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47846_length_1788_cov_17.572418"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="PL000_22480"
     CDS             <1..1731
                     /locus_tag="PL000_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91708.1"
                     /translation="GDKLDYFVFAEDFGAVSDGTATSGTDNSAAFAAAFAASDYVTVA
                     NRSGRYWLKNVDIPSGKTLDLRGAIVQPAAGADYCFKISERAQLRNGEFRDSPNNSLK
                     SITLTASAIAGDTTVTVDDPSIFYEGGLIVVVSDDDSGFTPSYGGPKLAVNHATVANI
                     AGSVITLSEPLQFDASSGNAALTSLGCVVVEGSNAVIQNLYITTVPLGVTVRSPVSGG
                     GIGKIRITDIEMNTARLGGIIELGSVSSATYDNIRGYLAAAGAPYVAAFGHKTDGSQA
                     AGTKGGHHLTRCDYLGTQEAFDFQDTEYVSLTDCYADTVSGVGVRVGQGTNDVLVTAQ
                     WASFCGKALSVSGAATRVYFYSCYTLASAPTVPWGQNIALEVEDGCTDIYINRDNWRR
                     SRAMRADPGWDTINNVTYMAVQHLAEDGTASAPARSWKLDTDTGWYRFQPGEERFQSN
                     GNTRFRVNIQGAGLQDGSALEPALFFAGNPDTGAYYDPSGQIRMTVNGLERWFWGDND
                     SGPITAPLRLPDVTLATANALTGLTDGQMIYITNGRKVGEGEGAGTGVMAYYSASSWR
                     VFSTDAAVTT"
BASE COUNT          389 a          459 c          500 g          440 t
ORIGIN      
        1 ggcgataaac tggattattt tgtgtttgcc gaagacttcg gcgccgtttc agacggcacc
       61 gctacttcgg ggaccgacaa ttccgctgct tttgctgctg ctttcgcggc aagcgattat
      121 gtgacggtgg ccaatcgcag cggacgatac tggcttaaaa atgtcgatat ccccagcggt
      181 aagacgctgg acttgcgcgg cgctattgtc cagcccgccg ccggcgccga ttattgcttt
      241 aaaattagcg aacgcgctca gttgcgaaac ggcgagtttc gagatagccc taataatagt
      301 ctcaaatcga tcacattgac ggcaagtgca attgctggcg acactactgt aactgttgat
      361 gacccgtcga tcttttacga aggcggcctt atcgttgtcg tgtccgacga tgacagcggt
      421 tttacgcctt cgtatggcgg tcccaagctg gcggtaaacc atgccacagt ggcgaatatc
      481 gctggcagcg ttataacgct gtccgaaccg ctccaattcg acgcgtcttc tggtaacgcg
      541 gctcttactt cgctcggttg cgtagtggtg gaaggctcta acgccgtaat tcagaatttg
      601 tacattacca ccgttccatt gggcgtcact gtcagatcgc ctgtatctgg tggcggtatc
      661 ggcaaaattc gcatcaccga tattgaaatg aacacggcac ggctgggtgg cattatagaa
      721 cttggttccg tttcgtccgc tacatacgat aatatccgtg gctatcttgc ggctgccggt
      781 gcaccatatg ttgctgcatt cggacacaag acggacggca gtcaggctgc tggaaccaag
      841 ggggggcatc atctcacacg gtgcgactat ctcggcacgc aagaggcttt cgattttcag
      901 gacacggaat acgtcagcct tacggactgc tacgcagaca cggttagcgg agtcggggtt
      961 cgcgtcggtc aaggaaccaa cgatgttctt gtaacggctc aatgggcgtc tttttgcgga
     1021 aaagcgctgt ctgtatcggg cgctgccacg cgagtgtatt tttactcttg ctacacattg
     1081 gccagcgctc ccactgtccc atgggggcag aatatcgcgc ttgaggttga ggacggttgt
     1141 acggacattt atatcaaccg cgataactgg cggcgttcac gcgcaatgcg cgccgatccg
     1201 ggttgggaca ccatcaataa cgtcacctat atggcggtgc aacatctcgc tgaagatgga
     1261 acagctagtg ctccagcacg ctcatggaag ctagatactg acactggttg gtatcgtttt
     1321 cagccgggcg aagagcgttt ccagtctaac ggaaacactc gattccgagt caacatccaa
     1381 ggcgctggtt tgcaggatgg ttccgctctg gaaccggcgc tgttttttgc aggtaaccct
     1441 gatacgggcg catactatga cccctcaggc cagatccgca tgacggtcaa tggtctggag
     1501 cgttggtttt ggggtgataa tgactccggg cctatcacag cgccgcttcg gctgcccgat
     1561 gtcacgcttg ctacagctaa tgctcttacg gggctgaccg acggtcagat gatctacatt
     1621 accaatggtc gtaaagtggg cgaaggcgaa ggcgccggca cgggtgtaat ggcatattat
     1681 tccgcttcaa gttggcgtgt attttcgact gatgcggctg tcacgactta aaagatgaat
     1741 tgaaagggcc ttctgatgag ccttacaaaa gcaacctatt caatggtc
//
LOCUS       DAOQLC010000341         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_48798_length_1770_cov_21.421574, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000341 DAOQLC010000000
VERSION     DAOQLC010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48798_length_1770_cov_21.421574"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1504)
                     /locus_tag="PL000_22485"
     CDS             complement(248..1504)
                     /locus_tag="PL000_22485"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNC91709.1"
                     /translation="MNEDESARGTRRYVALDIHKHYSVIAAVDREGRVVLHPVRVEHP
                     DLEGWLKKKLQPSDHVVFESTTNAWHVYDLLEPLVERVVVANPIKVKQIASARVKTDA
                     RDTLILARLLAANLVPDVWVPPAHVREMRQLLSQRRHLVETHTQIVNRMHSVAHRHHL
                     KHERGKRFNEKNTHWQKDKRLSKTEQFQLELEMENRAYTEKQIGRISKEVAKMSHQKP
                     WAEGMTYLMQLPGFGVITAMTVLAAIGEVERFESAKHLASYSGLTGGVDQSGTHLVQK
                     GITKEGRQELRWGMVEVAQRAVKSDPRWTRKFEELHKRMHRNQAIVAVARQLLELVWY
                     VLTRRQSYRHFSQERIAYKYLTWAWQMDEAARDGLTRQQFTRYYLMRLGIGQDLTRIA
                     LDPKHPRRIASEAEVLALRPELHRIE"
BASE COUNT          374 a          528 c          422 g          446 t
ORIGIN      
        1 atagatatat gtggtgggag aaaagatgaa cgagtcttcc aaccaacagc gcccaccgat
       61 taacagcagt gtcaagggat aaagagaaaa aaagaagaaa agtgattctg aaaccaaaaa
      121 caacccggat cacgggttgt tttttttcat ccctgtcgat cgattcccca accagagaca
      181 gccttcaccg cccccgccct cgtttctcag gatggggttg tcatgccgaa cgggaatact
      241 cttgttgtta ctcgatccga tgaagttcag gtcgtaatgc cagaacttct gcctcactcg
      301 cgatccgccg cggatgtttc gggtctaagg cgatgcgggt tagatcttgc ccgatcccca
      361 aacgcatcag gtaatagcgg gtgaattgct gccgggtgag accatcacgg gcagcttcat
      421 ccatctgcca agcccaagtc agatatttgt aagcgatccg ttcctgacta aagtgacggt
      481 aagattggcg ccgagtcagc acataccata ccagttccag caattgccga gcaactgcca
      541 caatcgcctg gtttcgatgc atacgtttgt gcagctcctc aaacttgcgg gtccagcgtg
      601 gatcggactt cacggctcgc tgcgccactt ccaccatccc ccaacggagt tcctggcgtc
      661 cttccttggt gatccccttt tgcacgagat gcgtaccgct ctggtccacg cccccggtca
      721 gacccgaata acttgccagg tgcttggctg attcaaaacg ctcaacctca ccgatcgctg
      781 ccagtaccgt catcgcagtg atcactccaa accccggcag ctgcatcaga taggtcatgc
      841 cctctgccca aggtttctgg tggctcattt tcgccacttc tttgctgatc ctcccaattt
      901 gcttttccgt ataggcacgg ttctccattt ccagttccag ttggaactgt tcggtcttgg
      961 acaaccgttt atccttttgc cagtgtgtat tcttctcgtt gaagcgcttc ccgcgttcat
     1021 gttttaagtg atgacgatgc gccacgctgt gcatccggtt cacaatctgc gtgtgcgtct
     1081 ccaccaggtg cctgcgttgg gataacagtt gacgcatctc acgcacatgc gcaggcggca
     1141 cccacacatc cggaaccaaa ttggcagcca gaagacgcgc caggattagc gtatctcgtg
     1201 catccgtctt tactcgcgca ctcgcgatct gcttcacttt gatgggattg gcaaccacca
     1261 ctcgttccac caatggctcc agcagatcat atacatgcca ggcgttcgtg gtcgattcaa
     1321 acaccacgtg atcgctcggc tgcaatttct ttttcaacca cccttccaga tctgggtgct
     1381 cgacacgcac tgggtggagc accactcttc cctcccgatc cacggccgcg atgacgctat
     1441 aatgcttgtg gatgtccagc gctacatagc gccgtgtacc tcttgcactt tcgtcttcat
     1501 tcatgctaga ctctccttgg gtttataggt tggggcgtgg gtttttgggt tgtaagtggt
     1561 acacaccgat tcgggctctt tgtcccagac gaggatccac cgggcaacca agtaacaaca
     1621 cgagttcaga agttcctgct tctctctcat gggcggtgtc ggtttgccct gatgctcaca
     1681 ccccggagca tccccggtga gattgtctcg cttaggatac tccgccacgc cccttcctgc
     1741 tacattctag taacaaagcg tgcacctgac
//
LOCUS       DAOQLC010000342         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_48965_length_1767_cov_24.620911, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000342 DAOQLC010000000
VERSION     DAOQLC010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48965_length_1767_cov_24.620911"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="leuD"
                     /locus_tag="PL000_22490"
     CDS             complement(<1..334)
                     /gene="leuD"
                     /locus_tag="PL000_22490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC91710.1"
                     /translation="MAQFTTLTSRALAIPVNDIDTDQIIPAQFLKVTDKNGLADALFY
                     NWRYNDDKSPKADFIINKPESQGAQIILAGDNFGCGSSREHAPWALTSWGIRAVISTS
                     FADIFRNNS"
     gene            complement(357..1085)
                     /locus_tag="PL000_22495"
     CDS             complement(357..1085)
                     /locus_tag="PL000_22495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91711.1"
                     /translation="MITDTVWKLPDIVNRYLTYRAAIPLAQEQIGVMMSILKTREQPI
                     ENFLDLGCGDGILGASILGSYPKARGVFVDFSEPMLTQARDVLKDYDGQLAFENLDYG
                     NPNWVTHMQTYAPFDAIVSGYSIHHQPDERKRDVYKEIFSLLKPGGWFVNVEHVSSAN
                     EVNVNLFEKHYTSVRHAIETQNGGTRTLQEIEHEYRTRPDKAANILAPVEDQCKWLRE
                     IGYEKVDCFMRIYELAIFGGCRPN"
     gene            complement(1105..>1767)
                     /locus_tag="PL000_22500"
     CDS             complement(1105..>1767)
                     /locus_tag="PL000_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNC91712.1"
                     /translation="FAPQGADWDKAVAYWKTLPSDEGAVYDKSIVLNAADLEPMITYG
                     TNPGMGMKITDRIPTAESFTEASQKAAFEKAMTYMGLQPGQSLLGQKVDVVFIGSCTN
                     SRISDLRLAAGMLKGKKVADGLRLMVVPGSQDVKKQAEQEGLDKIFKEAGGEWREAGC
                     SLCIAMNGDNLLPGQYAVSTSNRNFEGRQGKGGRTFLASPVTAAATAISGVVTDPRTV
                     LS"
BASE COUNT          385 a          422 c          495 g          465 t
ORIGIN      
        1 gcgagttgtt gcggaagatg tctgcgaacg aggtggagat gacggcgcgg atgccccaac
       61 tggtgagcgc ccacggcgca tgttcgcggc tcgaaccaca cccgaagttg tctccggcca
      121 atataatttg tgcgccctgt gactcgggtt tgttgatgat gaaatctgct ttcggagatt
      181 tatcgtcgtt gtagcgccag ttgtagaaaa gcgcgtcggc gagaccgttc ttgtcggtca
      241 ctttgagaaa ttgcgcaggg atgatctgat ccgtgtcgat gtcgttgaca gggatggcaa
      301 gtgcgcggga agttagagtt gtgaattgag ccattttaat ttccgatttc tgatttttaa
      361 tttggacggc atccgccaaa gattgcgagt tcgtagatgc gcatgaagca atcgactttt
      421 tcatagccga tctcacgcag ccacttgcac tggtcttcta ccggcgcaag gatgttggcg
      481 gctttgtctg ggcgggttcg atattcgtgt tcgatctctt gcaaggtacg cgtgcctccg
      541 ttctgtgttt cgatggcatg acgaacggaa gtgtaatgtt tctcgaacag gttcacgttg
      601 acttcgtttg ccgatgaaac atgctcaaca ttcacaaacc atccaccagg ttttagcaag
      661 ctgaaaattt ccttgtacac gtcacgctta cgttcatctg gttgatgatg aatggagtag
      721 cctgaaacaa tggcatcgaa aggcgcgtag gtctgcatgt gggtaaccca gtttgggttg
      781 ccataatcga gattttcaaa agcgagctga ccatcatagt ctttcaagac atcacgcgct
      841 tgtgtgagca tcggttcgga gaaatccacg aacacgccgc gggctttggg gtaactgcca
      901 aggatggatg caccaagaat accgtcacca caaccgagat caagaaaatt ctcaatgggc
      961 tgttctctgg ttttgaggat cgacatcatc acaccgatct gttcttgcgc caatggaatg
     1021 gcagcacgat aggtgagata acgattgacg atgtcgggga gtttccaaac ggtgtcggtg
     1081 atcatggtgt ttacaggctg ttctttaaga caataccgtt cttgggtcag taacaacgcc
     1141 actgatggca gtcgctgccg cggtgacagg gctggctaag aatgtgcgtc cgcctttgcc
     1201 ttggcgtcct tcaaagttac gattgctcgt tgatactgca tattgcccag gtaataagtt
     1261 gtctccattc atggctatac ataaactgca gcctgcttcg cgccattcac cgccagcttc
     1321 tttgaagatc ttgtccaagc cttcctgttc ggcttgcttc ttaacatcct gcgaaccggg
     1381 gacaaccatg agccgaagtc catctgcaac tttcttccct ttcaacatgc cagcggcgag
     1441 acgcaggtca gagatgcggg agttggtgca ggagccgatg aagaccacat ccactttttg
     1501 accgagcagc gattgaccgg gctgcaagcc catgtaggtc atggcttttt cgaaggcggc
     1561 tttttgcgag gcttcggtga atgactcagc ggtgggtatg cggtcagtga tcttcatgcc
     1621 catgccagga ttcgtgccgt aggtaatcat cggttcgaga tcggcggcgt tgaggacgat
     1681 ggacttgtcg tacacggcac cttcgtcgct gggcagggtc ttccagtagg cgacggcttt
     1741 atcccagtcg gcgccttggg gagcgaa
//
LOCUS       DAOQLC010000343         1767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_48968_length_1767_cov_13.339953, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000343 DAOQLC010000000
VERSION     DAOQLC010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48968_length_1767_cov_13.339953"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1557)
                     /locus_tag="PL000_22505"
     CDS             complement(139..1557)
                     /locus_tag="PL000_22505"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91713.1"
                     /translation="MHRKVFTILLSAVFILTQFNMAAAATVAGTASITSIPEFSIGKG
                     ADIDWAVTGMAAGTKNILIWAREVPAAAYALTECQLVATINGTSDASGTFSYDFTTTT
                     LALANADQVEFTITVDDQDCGGAPTLPVMDSVMGTTYIDNSAPNYFHGNPPSTTGINP
                     LLDTAACNTFEYWALAFDNSLLSPTMPYSGFNQWNPSVTGTFATPAPAGPASSLLSWV
                     FTFPSTASGMWTATIQPEDMAGNTNPVTGFRSFVVPTVAEKTECATFSDVSGHEDEVY
                     IRYLATLGLISGFADGTFGPDNTLTRAEAATLFEISNGYDSTTLPTSAPAGCDFTDVT
                     VSDWFAGWVWQACDDGFMNGVGGGLFDPNNLLTRGQVVTIFNNIYPAYIANPAAFGGY
                     IITPNPLITVYSLPAFGTGYGYRQAAWTDVSVGAYYTVPVQKAYGWGIADGTSETTFS
                     PDQAATRGEFAKMLYRALSKIH"
BASE COUNT          465 a          376 c          512 g          414 t
ORIGIN      
        1 atttacacat ttcggctgag ggcgcagtga agcatcttac ctaacttgtc acgggcgtac
       61 agtactgggt tatgacaata aaaatgggcg gacccatata tgggcccacc caaaacgaaa
      121 tgtgattttc tgaaaaaatt agtgtatttt gctcaaagca cggtaaagca tttttgcgaa
      181 ttcaccgcgg gtggctgcct gatccggcga gaaggtggtc tcactggtac catcagcaat
      241 gccccagcca taggcttttt gaaccgggac agtatagtat gctcccacgc ttacatcggt
      301 ccatgcggct tggcgatatc cataacctgt gccgaaagca ggaagagagt agaccgtaat
      361 caatgggttt ggtgtaatga tgtatccacc gaaagcagcg ggattggcaa tataagccgg
      421 gtaaatgttg ttgaagatgg tgacgacctg tccacgggtg agcaggttgt tggggtcgaa
      481 gagaccaccg ccaacaccgt tcataaagcc atcatcacaa gcctgccaga cccagcccgc
      541 gaaccagtct gaaacagtga catcagtgaa atcacagcca gcaggggcgg aggttgggag
      601 ggtggttgag tcgtacccat tggagatctc aaagagggtg gcagcttctg cgcgggtcag
      661 ggtgttatcg gggccgaatg taccatcggc gaagccgctg atcaagccaa gggtggccag
      721 gtaacggata tacacttcat cttcatgacc actcacatcg ctgaaagtgg cacattcagt
      781 tttttcggct acggtgggta ccacaaagct gcggaagcca gtgacgggat ttgtgttgcc
      841 agccatatct tcaggttgaa tggttgcagt ccacataccg ctggcggtgg atgggaatgt
      901 aaacacccat gaaaggagtg aacttgcagg tccggcaggt gcaggtgttg caaaagtccc
      961 agtgacggat ggattccatt gattaaaccc ggaatacggc atggtgggag ataacaggct
     1021 gttatcgaac gcgagtgccc agtactcaaa tgtgttgcag gcggcggtat ccaaaagcgg
     1081 gtttatgccg gttgttgaag gcgggtttcc gtggaagtaa ttgggagcgg aattatctat
     1141 gtaagtggtg cccataacag agtccataac tgggagggtg ggagcacccc cacaatcttg
     1201 atcgtccact gtgatggtga attcgacctg gtctgcattg gccaaagcca gggtggtagt
     1261 agtgaaatca taagagaaag taccggaggc atcgctggtg ccgttgatgg tggcaacaag
     1321 ttgacattcg gtcaaggcgt aggcggcagc ggggacttcg cgtgcccata tgaggatgtt
     1381 cttggtgccg gcagccatgc cggtgacggc ccaatcaata tctgcacctt tgccaatgct
     1441 gaactcaggg atggatgtga tggaagctgt gcctgctaca gtggcggcag cagccatgtt
     1501 gaattgggtc aatatgaaaa cagcggataa caaaatggtg aagactttcc tgtgcattac
     1561 atgctcctga aataatggta gtgtgttttt gaattttgag ttcaaaaagc ggctgtattt
     1621 tatggaattg agtctctatg gtcaacgaaa agaataggaa ataagtcctg gagattatcc
     1681 cctacattgg tttcatctta atgaatcaaa aagggtggac ccatatatgg gtccacccaa
     1741 aacgaaatgt gacttgcgtg aagatga
//
LOCUS       DAOQLC010000344         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_49272_length_1761_cov_28.728019, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000344 DAOQLC010000000
VERSION     DAOQLC010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49272_length_1761_cov_28.728019"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..976)
                     /locus_tag="PL000_22510"
     CDS             complement(14..976)
                     /locus_tag="PL000_22510"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNC91714.1"
                     /translation="MNIINQFVNGPIAIYNADCVEFMHSMLPENSVGYSIFSPPYSNL
                     YAYSNSERDIGNCRNDTEFFHHFKFVIDGLKRVLMEGRNVSVDVMNLPAMKSRDGYIG
                     LKDFRGQIIAAFIDAGFIYHSEHVIWKDPLIEATRTKAIGLMHKQLCKDSALSRAGLP
                     QYLLTFRKPGENKQPVAHEHGLTQFIGEDEPTEGNLSHNRWRKYASPVWMDIRSSRTL
                     NYRGGRDDEDERHICPMALDIIERGIELWSNPGDIVYDPFGGIGSTVRSAVNMRRKGM
                     STELKPSYFAMSVSNMDGVRQFIDNDTLEIFHAFDDQPNCTEIL"
     gene            complement(976..>1761)
                     /locus_tag="PL000_22515"
     CDS             complement(976..>1761)
                     /locus_tag="PL000_22515"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNC91715.1"
                     /translation="EMLAMFFTHDGGDTSKWRLKGHGKTKFWEWLATWAAFIRNPADL
                     GFDGSRYNLPPMRLVEHRIEVNDPVANTMTERRIARKNSVLMRCATLADVVNNSNEHW
                     VIWCNLNEESSMLASMIPDAIEVYGSQSPTIKENLLHKFSRGEARVIVSKPKICGFGL
                     NWQHCRNVAFVGIDDSFEKYYQAIRRCYRFGQSREVNVHLVYSSAEGAVKENLERKQQ
                     MADIMADQMVNHMREFTKREVMGTRMERTEYNPNQRINLPEWI"
BASE COUNT          404 a          453 c          431 g          473 t
ORIGIN      
        1 gagctacggg catttacagg atctcggtgc aattcggctg atcatcgaac gcgtggaaaa
       61 tttccagcgt atcgttgtcg ataaattggc gaacgccatc catgttgctg acactcattg
      121 cgaaatagct cggcttgagt tcggttgaca tacctttgcg tcgcatattt actgccgatc
      181 ggacagtgga cccgatgccg ccgaacgggt cgtatacgat atcgccaggg ttcgaccaca
      241 gctcaattcc acgctcgatg atatccaacg ccatcgggca aatgtggcgt tcgtcttcgt
      301 catcacggcc gccgcggtag ttcaaagtgc gagagctgcg aatgtccatc cataccgggc
      361 tggcgtactt tctccatcga ttgtgagaca gattgccttc ggtcggttca tcttccccga
      421 tgaattgcgt caggccatgc tcgtgcgcaa cgggttgctt attttcgcca ggcttacgga
      481 atgtcaaaag gtactgagga agtccggcgc ggcttagagc gctgtctttg caaagttgct
      541 tgtgcatcag gccgatagct ttagtgcgag tcgcctcgat cagcgggtct ttccaaatga
      601 catgctcgga atgataaata aatccggcgt cgataaatgc cgcgataatc tgcccgcgga
      661 aatccttaag cccaatataa ccgtctcggc ttttcattgc gggcagattc atcacgtcga
      721 ccgacacgtt gcgaccttcc atcagtaccc gcttgagccc gtcaatgacg aatttgaaat
      781 gatggaaaaa ctcagtgtcg tttcggcaat ttccgatatc gcgctcgctg ttgctatacg
      841 cgtaaagatt gctatatggc ggagagaaaa tcgaatatcc gaccgaattc tcgggaagca
      901 tgctgtgcat gaattcgacg cagtcggcgt tataaatggc aatcggacca ttcacaaatt
      961 gattgatgat gttcattaga tccactccgg caggttgatt cgttgattag ggttgtattc
     1021 ggtgcgttcc atgcgagtgc ccatgacttc gcgcttagta aattcacgca tgtgattcac
     1081 catttgatcg gccatgatgt cggccatttg ctgctttcgt tccagattct ctttgactgc
     1141 gccctcagcg ctcgaataga caagatgcac attcacttct cgggattgcc cgaatcggta
     1201 gcaacgtcgg atggcctgat agtatttctc aaacgaatcg tctatcccga caaatgcgac
     1261 atttcggcaa tgctgccagt tgagtccaaa cccgcaaatt ttaggcttac tgacaatgac
     1321 gcgagcttcg ccccgactaa atttatgcag cagattttcc ttaattgtcg gggactgtga
     1381 tccgtaaact tctatagcgt ccgggatcat gctggcgagc attgatgatt cttcattcaa
     1441 attgcaccag attacccaat gttcgtttga attgttgaca acatccgcaa gtgtcgcgca
     1501 tcgcatcagt accgagttct tgcgggctat tcgccgttcg gtcatcgtat tagcgaccgg
     1561 atcatttact tcgatgcgat gctcaacaag tcgcattggc ggcaagttgt atcggctacc
     1621 atcaaaccca agatcggcgg gattccgaat gaatgctgcc cacgtagcga gccattccca
     1681 aaattttgtt ttaccgtgac ctttgagtcg ccatttgctc gtgtcacccc cgtcgtgcgt
     1741 aaagaacatc gccagcattt c
//
LOCUS       DAOQLC010000345         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_49909_length_1749_cov_27.773318, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000345 DAOQLC010000000
VERSION     DAOQLC010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49909_length_1749_cov_27.773318"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PL000_22520"
     CDS             complement(<1..500)
                     /locus_tag="PL000_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91716.1"
                     /translation="MKKQSLFLIFVIALTSCTSLTKTPSPSSDPSGDPTQAPASEVET
                     SWQTYTNAEAGFSIQYPADWQEQDMPDTNDGQQHHITLQGPEGTVELVWGMGLGGACP
                     EGYQAISVAQGTWPACHSQKEDGTDFWSLAAQPIGDIGFTGFIYTNDTTAKSKETVLQ
                     VISTLS"
     gene            complement(792..1169)
                     /locus_tag="PL000_22525"
     CDS             complement(792..1169)
                     /locus_tag="PL000_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91717.1"
                     /translation="MKLNHLLRIHAFLAFAYAFLLAIFPQFTISILSSQPLNPLSTDI
                     TRILGAAILFVGLMALGASKLNSSSERRMIVTGLLIYTALGAVISLIGQLAGNWGVLG
                     WSNFGVYFLVALGYSIFLFQKHE"
     gene            complement(1278..>1749)
                     /locus_tag="PL000_22530"
     CDS             complement(1278..>1749)
                     /locus_tag="PL000_22530"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC91718.1"
                     /translation="RKNMITIEFLADHLDTIPTLTKWFRAQWGNYYANWSDEEMTKDF
                     LEDVSRNSLPSRLVAFDSNEIVGTIILRESEGETLLEHKPELGGLYVAESHRGQGVGT
                     ELVQAGMKLARKQGYSEISATTVRAAGILNRLGWEFIKTVQYPDGEVSLYRCKL"
BASE COUNT          476 a          361 c          413 g          499 t
ORIGIN      
        1 aaactgaggg ttgagatcac ctgaaggaca gtttccttgc tctttgcagt tgtgtcgttt
       61 gtgtagataa accctgtaaa accgatatca ccgatgggtt gagcggccag actccaaaag
      121 tctgttccat cttctttttg actgtgacat gcaggccagg tcccttgtgc aacggagatc
      181 gcttgatagc cttcagggca ggctccgccc agacccattc cccagactaa ttccactgtg
      241 ccttccgggc cttgcagggt gatgtggtgc tgttgaccgt cgttggtgtc aggcatatcc
      301 tgttcctgcc aatctgcagg gtattgtatg ctaaaaccag cctcggcatt cgtgtatgtt
      361 tgccaggatg tctcaacctc tgaggcgggt gcctgcgtgg gatcgcctga tggatcggaa
      421 gagggtgatg gggtctttgt cagggaggta caagatgtca gggcaatgac aaagatgagg
      481 aatagggact gctttttcaa tttatgctcc ttggagattt tgaaataacc gcatcaaatt
      541 gcattttgcg gatactttct gtttattatt tgaattttat tataaaggtg agtgttgaaa
      601 cggaaactag gatatttgta gggtgcagga atcatatagg tttcgatgga ttcaactgca
      661 aggaaggagt ggctggctct agaaaaaaag ataaggcacc attgacaacg gcgccttatc
      721 ttttgtgatg atctttgtat tgaagttcgg atcaagaaaa gaaagggaaa gatcgaattc
      781 tccaaatatt tctattcatg cttttggaac aagaaaatac tgtatcctaa agcgactaaa
      841 aagtacacac caaaattgct ccaaccaaga acgccccaat tgccagccag ttggccaata
      901 agggaaataa cagccccaag ggcagtgtat atcaataacc cagttacgat cattctgcgt
      961 tcagaagaag aatttagttt gctggctccc aaggccataa gacctacaaa aagaatcgcg
     1021 gctcccagaa tcctggtaat gtcggtgcta agaggattta gtggttgact cgacaagatt
     1081 gatattgtaa attgtggaaa aatcgctaaa aggaaagcgt atgcaaaggc taaaaaggcg
     1141 tggattcgta ataggtggtt gagtttcata agattaatgt tttcctttgt ataagtttat
     1201 aaagatgtag acaacggaac agattgtatt acattttttg tttgatggct tgactgacat
     1261 gttttatttt acagcattca tagtttacaa cgatacaatg agacttcccc gtctggatac
     1321 tgaaccgtct tgatgaattc ccagcctaaa cggttcaata tgcctgctgc tctaacggtt
     1381 gttgcagaaa tctccgaata tccttgttta cgggctagtt tcatcccggc ttgaaccaat
     1441 tcagtgccaa cgccctgacc tcgatgagat tcagcgacat acaggccccc taattcaggt
     1501 ttgtgttcca ataatgtttc cccctcgctt tcccgcagga taattgtgcc gacaatctca
     1561 ttcgaatcaa aagcaacaag ccgggagggg aggctgtttc gagagacgtc ttcaagaaaa
     1621 tctttcgtca tctcttcgtc tgaccagttt gcgtaataat tcccccattg agcacggaac
     1681 cattttgtca atgttggtat ggtatcaagg tgatcggcaa ggaactcaat ggtgatcatg
     1741 ttcttcctc
//
LOCUS       DAOQLC010000346         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_50181_length_1744_cov_15.661338, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000346 DAOQLC010000000
VERSION     DAOQLC010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50181_length_1744_cov_15.661338"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1744)
                     /locus_tag="PL000_22535"
     CDS             complement(<1..>1744)
                     /locus_tag="PL000_22535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC91719.1"
                     /translation="LLKITEDRAAELAIINSVQEGLASKLDMQAIYDLVGNKVCEIFN
                     LQTCFIMLHDKQTDMELYPFLVEGGMRLTQEPIPHDENGFGPFVMRNRQPLMINEKMT
                     ERSMEVNSYVIGGDDAREPKSAIYVPLLLGNEAIGVISVQNIEREHAFTDSDLRLLTT
                     LASSLSVALESARLFNETERNASQMATIANVGRELSSTLEMEQVIHIVTENVHNLFNA
                     RDTILRLLDEDGKNLATKVALGKYSAQNIASTLEMGKGVTGAIAESGIAEVVEYLHLD
                     PRRQHVAGTPDEDENPETLMVAPLIASNKTIGVISVYKDRRDGTFTPIDLDFLVGLGR
                     HAAIAIENSRLFDESQHLRDAAEHANQAKSTFLANMSHELRTPLNAIIGFTRIVRKKA
                     DGNLPDKQLENLDKVLSSSEHLLGLINTVLDIAKIEAGRMDVLPSSFDLGSLTETCVH
                     LATPLLKSGVALTRDLDPTLNTIFSDQDKIKQIILNLLSNAAKFTHHGQIHLSTQKHE
                     AMFRVHVADTGIGIGQEALDKIFEEFQQADSTTTRQYGGTGLGLAISRNLARLLGGDL
                     TATSEPGKGSTFTLS"
BASE COUNT          339 a          397 c          567 g          441 t
ORIGIN      
        1 aactaagtgt aaacgtggag cctttgccag gttcgctggt ggcggtgagg tcgccgccga
       61 gcaggcgggc aaggttgcgg ctgatggcaa ggccgagacc tgtgccgccg tattgacggg
      121 tggtggtgga atctgcctgt tggaattctt cgaagatttt atcgagcgcc tcttgaccga
      181 tgccgatgcc tgtatctgcc acatggacac ggaacattgc ctcatgcttt tgtgtgctca
      241 aatggatctg cccatggtgg gtgaatttgg ctgcgttgct gagtaggttg agaatgatct
      301 gcttgatctt gtcctggtca gagaagatcg tgttgagggt ggggtcgaga tccctggtga
      361 gcgccacgcc tgatttgagc aggggcgtgg caaggtgaac acaggtctca gtgagagaac
      421 cgaggtcaaa actggagggg agtacatcca tgcggccagc ttcgatcttg gcgatatcaa
      481 gcacggtgtt gatgaggccg agcaggtgct cggaactgga aagaaccttg tccagatttt
      541 caagttgttt gtctgggagg ttgccgtcag cttttttgcg gacgatgcgt gtgaaaccga
      601 tgatggcgtt gagcggggtg cgcagttcat ggctcatgtt ggcaaggaag gtgctctttg
      661 cctgattggc atgttcggcg gcatcgcgca gatgctgtga ctcgtcgaag agacgcgagt
      721 tttcgatggc aatggcggca tgacgcccca agcccacaag gaagtcgagg tcaatggggg
      781 tgaaggtacc gtcgcggcgg tctttgtaaa cggagatgac gccgatggtc ttgttgctgg
      841 cgatgagcgg ggccaccatc aatgtttcgg ggttttcatc ttcatcggga gtgcctgcca
      901 cgtgctgacg gcgcgggtca aggtgcaggt attcaaccac ttcggcaatg ccagattcgg
      961 cgatggcccc tgtaacgcct ttgcccattt ccaatgtgct ggcaatgttt tgggccgaat
     1021 attttccaag cgcaactttg gtggcaaggt ttttgccgtc ttcgtcgagc aggcgcagga
     1081 tggtatcgcg ggcgttgaag aggttgtgga cgttctcggt gacgatgtgg atgacctgtt
     1141 ccatttccaa tgtggaggag agttcacgcc cgacgttggc gatggttgcc atttggctgg
     1201 cgttgcgctc ggtctcgttg aagaggcgcg cgctttcgag cgccacgctc aatgaagatg
     1261 ccagggtggt cagcaggcgc aggtctgagt ctgtgaaggc gtgttcgcgc tcgatgtttt
     1321 gaaccgagat gacgccgatg gcttcattgc ccagcagcag ggggacatag atggcagatt
     1381 tcggttctct ggcatcgtcg ccgccaatga cataggaatt gacttccatg ctgcgttctg
     1441 tcattttttc attgatcatg aggggttgac ggttgcgcat cacaaatgga ccgaagccat
     1501 tctcgtcgtg cgggatcggc tcttgtgtca aacgcatacc gccttccacc aggaagggat
     1561 agagttccat atcggtctgt ttgtcgtgaa gcatgatgaa acaggtttgc aaattaaaga
     1621 tctcgcaaac cttgttgccc accaggtcgt agatggcttg catatccaat ttggaggcaa
     1681 gcccttcctg caccgagttg atgatggcaa gttcggcggc gcggtcttcg gtgatcttga
     1741 gcag
//
LOCUS       DAOQLC010000347         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_51164_length_1727_cov_32.489833, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000347 DAOQLC010000000
VERSION     DAOQLC010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51164_length_1727_cov_32.489833"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1232)
                     /locus_tag="PL000_22540"
     CDS             complement(33..1232)
                     /locus_tag="PL000_22540"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91720.1"
                     /translation="MLERLLRYADKQLHFGELVTGVRDHRRWSHVPTADVVTAVLDMF
                     FARLGSLNALEKLNHLPAWKKLPARSVSADTIGRVVDVLDDGSLRASQKMLYTSMKRN
                     KQLVAPSHGLIALALDGHESHCTYNQRCQGCLERDIDGKIQYYHRNVTAQLIFDNFSF
                     LVDMEEQRPGEGELTAAKRLYERVVRDYPRAFDVVLGDALYTNAPFINKVLDSGKHLI
                     TVLKDERTNIYQQADFAFANCEPAVIFENGSKSYSCWDEGNFRMPDVSSALRIVKSEE
                     STRIKAQLDSSLHIKTNTWMWITSLPSIRAGTRTVHALGHARWNIENQGFNELSNHWC
                     SDHIYKHSPGAIRNFLLLCMIAHNIFQCFYRRALKPALQKALTEVLVSLQVAADLCGD
                     WPRCHSP"
     gene            complement(1236..1580)
                     /locus_tag="PL000_22545"
     CDS             complement(1236..1580)
                     /locus_tag="PL000_22545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91721.1"
                     /translation="MTDNHSHLPPNLRNASEFKLRAKLRELLKDGNILHATLNQRFRV
                     CGKKNCKCASGERHEALYLVTRQGGKLRQLFVHPDFHEDVRQWVANYQILLQLIDELS
                     EFSWQRVADREM"
BASE COUNT          416 a          419 c          477 g          415 t
ORIGIN      
        1 cagggctgaa atttgccggg aagtaatatt ccctatggcg aatggcaccg cggccagtct
       61 ccacagagat ccgccgccac ttgcagggac accaaaactt cagtcaacgc tttctgcaat
      121 gctggtttca gcgccctcct gtagaagcat tgaaatatgt tgtgagcaat catgcaaagc
      181 aagaggaagt tgcgaatggc tcctggacta tgtttgtaga tatggtcgct gcaccagtgg
      241 ttggagagtt cgttaaagcc ctggttttct atgttccatc gggcatggcc aagggcatgt
      301 actgttctcg taccagccct tatgctcggc agagaagtaa tccacatcca ggtgtttgtt
      361 ttgatgtgca gggaagaatc aagctgagcc ttgattctgg tggactcttc ggatttgaca
      421 atgcgcaatg cgctagagac atccggcatg cgaaagtttc cttcatccca gcaggagtat
      481 gatttggagc cattttcgaa gatcacagcc ggttcgcaat tggcgaaagc gaagtcggct
      541 tgctggtaga tattggtgcg ctcatctttc aaaaccgtga ttaaatgctt gcccgagtcg
      601 aggaccttgt taatgaaagg ggcattggtg tagagcgcgt caccaagcac cacatcaaat
      661 gcacggggat agtccctcac tactcgctca tacagtcgct tggccgcagt caactcacct
      721 tctccaggcc gttgctcttc catgtctacc agaaacgaga aattgtcgaa aatcagttga
      781 gctgtaacat tacggtggta gtactgaatc ttgccgtcga tatcgcgctc aagacagccc
      841 tggcagcgct gattgtaggt gcagtgagat tcatgaccat caagagcaag cgcgatcaga
      901 ccgtgtgaag gtgccacgag ttgcttgttt ctcttcatgg aagtgtagag catcttctgg
      961 gaagcgcgca gagagccatc atcgagaaca tccacaacac gtccgatggt atcggcactg
     1021 actgagcggg ccggtagttt cttccaggcg ggaagatgat tgagcttctc cagagcattg
     1081 aggctgccga ggcgggcgaa gaacatatca agcacggccg tcaccacatc agcggttggc
     1141 acgtgggacc agcggcggtg gtctcgaaca ccggtgacaa gctcgccaaa atgcagttgc
     1201 ttgtctgcgt agcgcagcaa tcgctcaagc attcatcaca tctccctgtc ggcaacgcgc
     1261 tgccaggaaa actcggaaag ctcgtcaatt agctgcagaa gaatctgata gttggctacc
     1321 cactggcgaa cgtcttcgtg gaaatcggga tgaacaaaga gttgacgaag cttgccgcct
     1381 tgcctggtga ccaggtagag agcttcgtgg cgttcgccgg aagcgcattt gcagttcttt
     1441 ttgccgcaga cgcgaaaacg ttgattgagg gtggcatgaa ggatattgcc atcctttaga
     1501 agttcgcgaa gtttagcccg aagcttaaat tcggaagcgt tgcggaggtt gggaggaagg
     1561 tgggagtggt tgtcagtcat ggtttcgcct cgatcgagta tatacgcatt ataccagatt
     1621 tatttgcgaa cggcgaatgc gaatctgctc tcagcgatca gaagtttcta atccctaatt
     1681 gccactgcgt ctaagcccgg ttcaacagcc actctgcgga atcgctg
//
LOCUS       DAOQLC010000348         1721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_51504_length_1721_cov_13.956783, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000348 DAOQLC010000000
VERSION     DAOQLC010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51504_length_1721_cov_13.956783"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1610
                     /locus_tag="PL000_22550"
     CDS             90..1610
                     /locus_tag="PL000_22550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNC91722.1"
                     /translation="MKPRIVDENEWTWTILGLALLFGIFVRFIPGPLAGLPINDGGMF
                     AVMIRDLKANQFVLPVLTSYNHAAIPFAYPPLGLYLGAFFELLGLSEFQMMIWLPAFL
                     TIATVPLFYLLALELLGNRPRAALATVFFALAPGNYVWYLMGGGLTRALGAVFYMLAL
                     YFVHRAFREADWRLVFFATLCCALTVLSHPQAALLLVLGCAVFWLFSGFSRSSMLRAF
                     VIAAGTALLTSPWWGVVIARHGVEVFLSAGQSGDLKASLSALLGNVLSRQTILPFSTF
                     FWLLGLFGAIYKRRFDLLVWGFAAYLVDQRSASIVTSFLYPMLAAYGFMDVTPTLVNW
                     FRTRRWSNELDNALFNRRGLSMSLLGIIFYLLIECFVHAYVIRNVTLPYAAQDMMAWV
                     THNTPPDSRFLILTGHVDAMTDPVQEWFPALADRHSVTTLQGLEWLLRGDFVTRWDQL
                     SALQRCEETGCVTAWAEEIGLEFDHLVVDASNVSVDMFLSQGYSVVFDNGRYVVLK"
BASE COUNT          306 a          369 c          491 g          555 t
ORIGIN      
        1 tggaaaattt tgtggattat agtcacctgt gggtggattg catagcacat aaaattctgg
       61 atggtagaat ttgttttatg cgatctgaca tgaaaccccg aattgtggat gaaaatgagt
      121 ggacctggac gatcctcggt ctggcgctcc tgtttggaat ttttgtgcgc ttcatccctg
      181 gacctttggc tgggttgccc atcaatgacg gtgggatgtt tgccgtaatg attcgtgacc
      241 tgaaagcgaa tcaatttgtg ctccctgtgc ttacatcgta caaccatgcg gcgatccctt
      301 ttgcatatcc accgctggga ctttatttgg gagcgttttt tgaattgctg ggcctttctg
      361 aattccaaat gatgatctgg ctgccagctt tcttgaccat tgccacggtc ccattgtttt
      421 acctgttggc actggagttg ttgggaaatc gtccacgggc ggcactggct actgtgttct
      481 ttgctttggc tccggggaac tatgtctggt atctcatggg tggcgggttg acccgtgcat
      541 tgggtgctgt cttttacatg ctggctttgt atttcgttca tcgcgcattc agggaggccg
      601 attggcgttt ggtctttttc gccacccttt gctgtgcgct gacggttttg agtcacccgc
      661 aggcggcttt gctgcttgta ctgggttgtg ctgttttctg gctttttagt ggtttctctc
      721 gttcgtccat gctgcgtgct tttgtcattg cggctggaac ggctttgttg acctctccat
      781 ggtggggtgt ggtgattgca aggcatggtg tggaagtgtt tttatccgcg ggtcaatcgg
      841 gcgatttgaa ggcatccctt tcagctttgc tgggaaatgt attgtcacgg cagaccattc
      901 tcccattttc gacctttttc tggttgttgg gtttgtttgg ggcgatctac aagcgaaggt
      961 ttgatctgtt ggtttggggg ttcgcagcct atttggttga ccaacgcagt gcctcgatcg
     1021 ttacttcgtt tttgtacccc atgttggccg cgtacggatt tatggatgtg accccgacgc
     1081 tggtcaactg gtttcgtacc cgaagatgga gcaacgagtt ggataatgcc cttttcaaca
     1141 ggcgtggatt gtccatgagt ttgctgggga tcatttttta tctgcttatt gaatgttttg
     1201 tccatgcata tgtgatccgt aatgtaaccc tgccgtatgc cgcgcaggat atgatggcat
     1261 gggtgacgca caacacacca ccagacagca gattccttat tttgaccggg cacgtggacg
     1321 cgatgaccga cccggtgcag gagtggttcc ctgctttggc agaccgccat tctgtgacca
     1381 cgctgcaagg gcttgagtgg ctgttacggg gagattttgt cactcgctgg gatcaacttt
     1441 cggctttgca gcgatgcgaa gaaacgggtt gtgtcactgc ctgggcggag gaaatcggct
     1501 tggagtttga ccatcttgtt gtggatgcat ccaatgtttc tgtggatatg tttttgtcgc
     1561 agggatattc tgtggtgttc gataatggac ggtatgtcgt tttaaaataa aaaccgcaga
     1621 ggacatcttc ctctgcggtt ttgctgttta gaaaatctat cggatggttc ccagcaggcg
     1681 cggataccaa taccaaagta tatcggcggc gggtttgttg t
//
LOCUS       DAOQLC010000349         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_52197_length_1709_cov_17.758767, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000349 DAOQLC010000000
VERSION     DAOQLC010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52197_length_1709_cov_17.758767"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..84
                     /locus_tag="PL000_22555"
     tRNA            8..84
                     /locus_tag="PL000_22555"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Ile,seq:gat)
     gene            164..239
                     /locus_tag="PL000_22560"
     tRNA            164..239
                     /locus_tag="PL000_22560"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ala,seq:tgc)
     gene            628..>1709
                     /locus_tag="PL000_22565"
     rRNA            628..>1709
                     /locus_tag="PL000_22565"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          423 a          383 c          567 g          336 t
ORIGIN      
        1 gtgacgaggg ctagtagctc agttggttag agcgcgcgct tgataagcgt gaggtcggaa
       61 gttcaaatct tccctggccc accagattga gtgcgacggc tctgccggct ggagtgcgag
      121 ggaccatctg ttcggatccg ggacttggat ccattcaaga gtgggggccg tagctcagct
      181 gggagagcgc ctgctttgca agcaggaggt catcggttcg atcccgttcg gctccaccag
      241 tttcctggtt cacaggacct ctgcagaaag aagaaaattg cgcatgagag agcggacgaa
      301 agtctgcgct tatgcgttgg ctgtttgaca ttgttgatcg gatgcaaagc acgcttggac
      361 gcgtagtcac gcggacatgg tggcgagagc tgctgtgttt gtgatgagtg cgggtcgcga
      421 gcagtgcacg agtaaatgaa gtagctaagt tgtgccgagc cgttgcgcac gggacgcagg
      481 acgtgagagc tccgaaggtt ctcgcgtggt tctctacggt gtagagggcc gcagattgga
      541 tccgcagata caagagctgc ggtggtccag gtctcgtcaa cagacgaccc gctgtgcggg
      601 tgtttgtcac tgtgtgcgat gggttgtgat caagcgttta agggcatttg gtggatgcct
      661 tggcactgag aggcgatgaa ggacgtagca cgttgcgata agccgcgggg aggcgcgagc
      721 agcctttgat ccgtggatct ccgaatgggg caacccggcc gcaaggtcat ctcactctga
      781 atccataggg gtgagaagcg aacccaggga actgaaacat ctcagtacct ggaggaaagg
      841 acatcaaccg agactccgcg agtagtggcg agcgaaagcg gaccaggcca gttgcttcga
      901 tgttggaacc agaaccgtct ggaaaggcgg gccgtagtgg gtgatagccc cgtatgggta
      961 gtcaacgtcg aggcacttga gtaaggcggg acacgagcaa tcctgtctga agatgggggg
     1021 accaccctcc aagcctaagt actcctcagt gaccgatagt gaacaagtac cgtgagggaa
     1081 aggtgaaaag caccccgatg aggggagtga aatagaacct gaaaccgaat gcctacaagc
     1141 agtgggagcg gccttgagcc gtgaccgcgt acctcttgta taatgggtca gcgacttagt
     1201 cttacgagca agcttaagcc ggtaggtgga ggcgtagcga aagcgagtct gaatagggcg
     1261 aatgagttcg taggattaga cccgaaaccg ggtgatctag ccatggacag gttgaaggtg
     1321 gggtaacacc cactggagga ccgaaccaat taccgttgaa aaggtactgg atgatctgtg
     1381 gctaggggtg aaaggccaat caaactcgga aatagctggt tctccgcgaa atctatttag
     1441 gtagagcgtc agaggttcac cgtagggggt agagcactgg atgggctagg ggggagcgat
     1501 ccttaccaaa cctaaccaaa ctccgaatac ctacgggtta tactctggca gacagactac
     1561 gggtgctaag gtccgtggtc gagaggggaa gagcccagac caccagctaa ggtccccaag
     1621 ttatggctaa gtgggaaagg aagtgggaag gccaagacaa ccaggaggtt ggcttagaag
     1681 cagccatcct ttaaagaaag cgtaatagc
//
LOCUS       DAOQLC010000350         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_53664_length_1685_cov_4.411656, whole genome shotgun sequence.
ACCESSION   DAOQLC010000350 DAOQLC010000000
VERSION     DAOQLC010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53664_length_1685_cov_4.411656"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..553)
                     /locus_tag="PL000_22570"
     CDS             complement(347..553)
                     /locus_tag="PL000_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91723.1"
                     /translation="MIYVYADGYVGLHAYPTRDDYFTITDDYAGRVGELHCLLTKHFG
                     DTRQRDEVYAEALELLELGDSLSD"
     gene            complement(550..849)
                     /locus_tag="PL000_22575"
     CDS             complement(550..849)
                     /locus_tag="PL000_22575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91724.1"
                     /translation="MIEAVLLFAALTAAFELIIFAKLSPRVRLRVLNWPGAVTAAAFA
                     FNLWVHWGTITGSMTAVTAGMASMAVTSFARWYWGYIDNGKYIVGQRKFTIGELK"
     gene            complement(920..1288)
                     /locus_tag="PL000_22580"
     CDS             complement(920..1288)
                     /locus_tag="PL000_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91725.1"
                     /translation="MSRKAQASTQVQANEVVAKPSKAEQALAKQLAQLKAQNEVLMLE
                     REAAAQAKAKAEADAIEAAIQQRIADAVAKAKAEEQRVAEEAAAEEKAKVDAVLAKGK
                     TGLLYALAAGLGAATMLVAR"
BASE COUNT          383 a          404 c          458 g          440 t
ORIGIN      
        1 ggtggcacga ggattgcttg tgccaaaaag cacgattctg ccgatgtatt tcagcaaacg
       61 gcaggatttc ggcagagtcg gcgatggctt aaggacgggg gttctactct tattccttct
      121 attcttctat tctttataag aagaagaaga agaagaagaa gaagaagaag atcaaaaata
      181 tatagtaggt attttatata gagatagtat tttccccgag agtttgaaaa catttcggaa
      241 ggcggaatat tggaaggatt gcaggttttt gcgccagctc tgatcgggtg atcgtgagcc
      301 ggcgcatgtg cctgaatatc tgaacgagtg ttcagttgtg ggagtgttag tcggagagcg
      361 agtcccccag ttcgagcagt tcgagcgctt cggcgtacac ttcgtcgcgc tgacgcgtat
      421 caccgaagtg tttggttagt aggcaatgca actcgccgac gcgcccggcg taatcgtccg
      481 tgatcgtgaa gtaatcatcg cgggttgggt aagcgtgcaa gccgacgtat ccatctgcgt
      541 aaacataaat catttcaatt ctccaattgt gaacttgcgt tgaccaacga tgtacttgcc
      601 gttgtcgata tatccccagt accagcgggc gaacgatgta accgccatgc tggccatccc
      661 tgcggtcact gcggtcatgc tgcctgtgat ggtgccccag tgcacccaca agttaaatgc
      721 gaatgctgcg gctgtgactg caccgggcca attcaaaact ctgagccgaa cgcgtgggct
      781 caactttgcg aagatgatta gctcgaacgc cgcagtgagt gcagcgaaga gtaagacggc
      841 ttcgatcatg atgacctcct atgacattgt actactgact tatctacgaa gaagtgaaac
      901 ccccttgcgg gggcttgggt tatcgagcga ctaacatcgt ggctgcgccg agacctgcgg
      961 ccagtgcgta gagcagaccg gttttgcctt tggctaggac agcgtcgact ttcgctttct
     1021 cttcggctgc tgcctcttcg gcaacgcgtt gctcttcggc tttagcctta gcgactgcgt
     1081 cggcgatgcg ttgttggatt gccgcttcga ttgcgtcggc ttccgcttta gctttggctt
     1141 gtgctgctgc ttcgcgttca agcatgagca cttcgttctg tgctttgagt tgagcgagtt
     1201 gtttagctag cgcttgttca gctttgctag gttttgcaac aacttcgttt gcttgaactt
     1261 gggtagatgc ttgagctttg cgtgacatgg tgaatctcct agtgagtagc gagaaggatt
     1321 tctcacttac acaaggagaa gccagtggga gcgtagcgac taagcatcct tttctcctcc
     1381 gcccaaagcc aaatccgaag tggggtaccc ggttttgagc ggtgggggag gaggctccgt
     1441 ccccgcgcat ccaagcactt ttcacaaatc acaatagatt gtgtggtgag catccaggta
     1501 ccctatcccc tgccctgcaa attcaatccg aagtaccccc gtgccattta atctgacaat
     1561 ctgctaagat ttataaaaat tttttgcaga gaagaccatg acaaaagcag ccggcgtaac
     1621 cgctatcccg acgcagcagc agcaggagtt caattcgctt cgcctgaaga tcattgatgt
     1681 ggagt
//
LOCUS       DAOQLC010000351         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_53914_length_1681_cov_31.357319, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000351 DAOQLC010000000
VERSION     DAOQLC010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53914_length_1681_cov_31.357319"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PL000_22585"
     CDS             complement(<1..1105)
                     /locus_tag="PL000_22585"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91726.1"
                     /translation="MSTATPRAQFHQKNLRQPAQHAAVENQLHYERHRPEETTLYRLV
                     QEHIGTFFAQVETETGSGLPDFVKDEFEAFLECGILAHGFLRLRCANCAHEKLVAFSC
                     KRRGFCPSCGGRRMAQTAAHLVDHIIPRVPVRQWVLSLPIPLRYLLAAHPRLITPVLQ
                     VIHRAISTSLIKQAGLKRSEAQTGAITLIQRFGSAANLNIHLHCLVLDGVYRIQNGVA
                     EFHSARSPTTEQLQRLLSQIIQRIMKALTRNGALIEEEGMSYLAEMETDAALSPLQSA
                     ACTYRIALGRRAGQKVLTLKTISTQNTQPQENKKYCVNAHGFSLHAGVRCAMNQRKEL
                     EHLCRYITRPAIANERLTRNKDGQIVLKLKTPYR"
     gene            complement(1154..1327)
                     /locus_tag="PL000_22590"
     CDS             complement(1154..1327)
                     /locus_tag="PL000_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91727.1"
                     /translation="MQDLSLSHGKNGNILNAQVMQLMGNIKTMQRMACAHDTNNQLFF
                     HLGDQHLAKSLKA"
     gene            1488..>1681
                     /locus_tag="PL000_22595"
     CDS             1488..>1681
                     /locus_tag="PL000_22595"
                     /inference="COORDINATES: protein motif:HMM:NF023168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNC91728.1"
                     /translation="MERKAKVTQDAVTDACDELMESGKNVTVNAITAMTGGSFSTVGA
                     MVKNWKAEQALKTAPAIEMP"
BASE COUNT          386 a          410 c          453 g          432 t
ORIGIN      
        1 cccgataagg tgtcttcagc tttaacacta tttgcccatc tttattgcgt gtcagtcgtt
       61 cattagcaat cgccggtcgc gtgatgtaac ggcacagatg ttccaattcc ttacgctgat
      121 tcatggcgca acgcaccccg gcatgcaggc taaagccatg tgcattgaca cagtattttt
      181 tgttttcctg tggttgcgta ttttgtgtgg aaatggtttt taacgtcagc accttctgcc
      241 ctgcccggcg accgagtgct atccggtagg tgcaggccgc cgattgcagt ggactcagcg
      301 ccgcgtccgt ttccatttca gctaagtagc tcatcccctc ttcctcaata agcgcgccgt
      361 tacgggtcaa tgctttcatg atgcgctgga tgatctggct aagcagtctt tgcaactgct
      421 cagtcgtcgg tgaccgtgcg ctgtggaatt ccgccacacc attttggatg cggtaaacgc
      481 cgtcaagcac taggcaatgc agatgaatat tgagattagc cgccgaaccg aaacgttgaa
      541 taagcgtaat cgcaccggtc tgtgcttcgg atcgtttcaa tccggcctgt ttgatcagag
      601 aagtagagat ggcacggtga atcacctgca ataccggtgt gattaagcgc ggatgagcgg
      661 ctaacaggta gcgtagcggg atcggcagtg agagcaccca ttggcgcacc ggcactcggg
      721 gaatgatgtg atcaaccagg tgagcagccg tctgcgccat gcgccgccca ccgcacgagg
      781 gacaaaatcc acgccgcttg caggaaaagg ctaccagttt ctcatgggcg cagttggcac
      841 aacgcaggcg taagaaccca tgggccagaa tgccacattc aagaaatgcc tcaaattcgt
      901 ctttaacaaa gtcaggtaac cctgaaccgg tttccgtctc aacctgtgca aagaaagttc
      961 cgatgtgctc ttgcaccaac cgatacagag tggtttcttc cggtcggtgc cgttcatagt
     1021 ggagctgatt ttcaaccgct gcgtgttgag ctggttggcg caggttcttt tggtggaatt
     1081 gggcgcgggg tgtggccgtc gacatgatct ttgagaacga atgttttcgt tctcaaagta
     1141 tcgacttctc gggctaggcc ttgagtgatt tcgccagatg ttggtcgcct agatggaaaa
     1201 acagctggtt gttggtgtca tgggcgcagg ccatgcgctg catggttttg atgttaccca
     1261 tcagttgcat gacttgggcg ttcagaatgt tgccgttttt gccgtgggac agcgacaaat
     1321 cctgcaaaaa tctcgtgaaa gctgtggcgg atgccgtgtt tggcgttcta cccaatgttt
     1381 ccggattttt tcagccacaa ttccagcggc tggggattcg ctttgaaatg gggtgttaat
     1441 caacatctgc tactactatc tactattata ttcaatcagg tgatcctatg gaacgtaaag
     1501 cgaaagtgac gcaagatgct gtaaccgatg catgtgatga actcatggaa tccggtaaaa
     1561 acgtcactgt aaacgctatc actgcgatga caggaggttc cttttccacc gtaggcgcaa
     1621 tggtcaagaa ttggaaggcg gaacaagcct taaaaactgc accagcaatt gagatgccag
     1681 a
//
LOCUS       DAOQLC010000352         1677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_54162_length_1677_cov_28.091862, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000352 DAOQLC010000000
VERSION     DAOQLC010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54162_length_1677_cov_28.091862"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1579
                     /locus_tag="PL000_22600"
     CDS             176..1579
                     /locus_tag="PL000_22600"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91729.1"
                     /translation="MNKKVFTVLLLSIFILAQFGTASAIIVPGDAAITSLPDFSTDLS
                     VDVEWTVTGMAAGTKNILLWAREYANPANAYASADCQLVATINGTGDASGTFNYDYAL
                     STLALIYGDEVEFTITVDDMDCGGGPATPDIDSVMGITYIDNAAPVYIQGYPPSQVPT
                     LPDTHTYACNTFEFWAVATDGAGFVPAYSGFHAWNTFITGTFAPPAPIGEGDALLSWE
                     YTFPATASGLWTFGVEPEDKSGNIDKKDYFRDAVAIAPEELEECTTFSDVSGHADETY
                     IRYLATLGLISGFADGSFGPDATLTRAEAATLIETANGYDATMLPTSAPAGCDFTDVT
                     ASDWFAGWVWQACSDGIMNGVGGGLFDPNNLLTRGQIVTIFKNVSDTFLSSPLLFSGY
                     ISTFNPITFLYILPFTYRQSAWTDVSIGAYYAEPVVKAYGWGIVEGTSDTTFSPDQPV
                     TRGEFAKMLYRAISRID"
BASE COUNT          421 a          505 c          361 g          390 t
ORIGIN      
        1 cccgtgacaa gttaggtaag atgcttcact gcgccctcag ccgaaatgtg taaattaaaa
       61 caagaactaa ctgaaataga ttgactcaaa tcacaagagt ccatacaatg catctattca
      121 gacaaacgtc atccaaataa agcgtaatcc ataaaatctt aataaggagt gaaaaatgaa
      181 caaaaaagtc tttaccgttt tattgttatc catcttcata ctggctcaat ttggaacagc
      241 ctctgccatc attgtccccg gtgatgccgc catcaccagc ctgccagact tttccacaga
      301 cctcagtgtg gatgtggaat ggaccgtgac cggcatggca gccgggacaa agaacatcct
      361 cctttgggca agggaatatg ccaaccccgc caatgcgtat gcatcggcag attgtcaact
      421 cgttgccacc atcaatggca ccggcgatgc ttccggcacc ttcaactacg attatgccct
      481 ttccacattg gcattgatct atggtgacga ggttgaattc accatcaccg tagatgatat
      541 ggactgcggc ggcggtcccg ccacgcctga tatcgactct gtaatgggca ttacctatat
      601 tgataacgcg gctcccgttt atattcaagg atacccgcct tcacaagtac ccaccctgcc
      661 ggatacccac acctacgcct gcaacacctt cgaattctgg gcggttgcca cagacggcgc
      721 agggttcgtc ccagcctact ctggttttca cgcctggaac acctttatca ccggcacctt
      781 tgcaccaccg gcccccatcg gtgaaggaga tgccctgctt tcatgggaat acaccttccc
      841 cgccaccgcc agcggattgt ggactttcgg tgtggagcct gaagataaaa gcggcaacat
      901 cgacaaaaaa gattacttcc gtgatgcggt agccatagcc ccggaagaat tggaagaatg
      961 cacaaccttc agcgatgtca gcggccatgc ggatgagacg tacatccgct accttgcgac
     1021 ccttggcctg atcagcggct tcgccgacgg aagcttcggc cccgatgcaa cgctcacccg
     1081 tgctgaagcc gccaccctca tcgaaacagc caacggctat gatgccacca tgttacccac
     1141 ctctgccccc gctggctgtg acttcaccga tgtcactgcc tcagactggt tcgctggctg
     1201 ggtctggcaa gcctgctcag acggaatcat gaacggtgtc ggcggcggtc tcttcgaccc
     1261 gaataacctg ctcacccgtg gacagatcgt caccatcttc aaaaacgtct cagatacctt
     1321 tctatcaagt ccgcttctat ttagtggata catcagcacc tttaatccaa tcactttcct
     1381 gtatatcctt ccattcacct accgccaatc tgcatggacc gatgtttcca tcggcgcata
     1441 ctatgccgag ccggttgtga aggcctacgg ctggggcatt gtagaaggaa ccagtgacac
     1501 caccttctca ccagaccaac ccgtaacccg cggcgaattc gcaaaaatgc tctaccgtgc
     1561 catcagccgc attgactaac caaatctctt aaaccaattc actttgggcg aatccttcac
     1621 cggatccgcc cattttgttc tcaaaccaaa gtaccaattt ctgaaattgg ttacttt
//
LOCUS       DAOQLC010000353         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_54935_length_1665_cov_20.077640, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000353 DAOQLC010000000
VERSION     DAOQLC010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54935_length_1665_cov_20.077640"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1556
                     /locus_tag="PL000_22605"
     CDS             198..1556
                     /locus_tag="PL000_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC91730.1"
                     /translation="MSNYKKVDAYLEKNLDQNLEELKRYVAQPSISAQNIGLEECAQM
                     VKEMLEKRGFTAQIKSTGGAPVVFGERKGKSNKTLLIYNHYDVQPPEPLELWESPPFA
                     PEIRDGKMYGRGVSDDKSHLTSRLFALDAILDEDGELPCNVKFIVEGEEETSSVHLHD
                     FVRENMDLLKADACIWEFGGVDHREVPMQYLGLRGICYVELSVESLGTDVHSGLGGSI
                     FPNAAWRLVWALNSLKGQDERILIPGFYDGIIPPNERELELMKKLPDVADEYKKRFGV
                     KEFIKGIKGGNELKIEEVFTPTCTICGITSGYQGPGSKTVQPARASAKVDFRLVPAQM
                     PEDIVKKLRAHLDAQGFTDVKIDFLGGEPAARTNPDDPFIKLIVDTAADIYDSPMELV
                     PTVGGSGPNYPFVHDLGLPVATMGLGHPDTRAHAPNENIRLDLYLKHAKHMARVVKEF
                     SK"
BASE COUNT          462 a          417 c          394 g          392 t
ORIGIN      
        1 aggcacaaag tctcgaagac tcaaagaaaa aaaagcttag tgacttagtg ccttggtgac
       61 tttgtggtct acaacacttt acttttgcaa gaggtcaaat atcgcattac gagcagaccg
      121 cgaaacatca gtttatacga acacttctgt ttttagtatt tccttacgca cacagcaaaa
      181 aacaaaggag attcatcatg agtaactata aaaaagtaga tgcctacctc gaaaagaacc
      241 tcgatcaaaa tcttgaagaa ctcaaacgct acgtggccca acccagcatc agcgcgcaaa
      301 atattgggct ggaagaatgc gctcaaatgg tcaaagagat gcttgaaaag cgcggattca
      361 cagcacagat caaatccacg gggggtgcgc ctgtggtttt tggcgaacgc aaaggcaaaa
      421 gcaataagac cctgctcatc tacaatcatt atgacgtgca gccgcctgaa ccgttggaat
      481 tgtgggagtc accgccattt gcgcctgaga tccgcgatgg gaaaatgtat ggccgcggcg
      541 taagcgatga caagagtcat ctcacctcac gcctctttgc gctggatgca attctcgatg
      601 aagatggcga acttccctgc aatgttaaat ttatcgtgga aggcgaagag gaaaccagca
      661 gcgttcacct gcatgacttt gtccgtgaaa atatggatct tctcaaagcc gatgcctgca
      721 tttgggaatt tggcggtgtg gatcaccgtg aagtacccat gcaatatctc ggcttgcgcg
      781 gcatctgcta tgttgagctt tcggtcgagt cacttggcac agatgtccac tctgggttgg
      841 gtggcagtat cttccccaat gcggcatggc gactcgtttg ggcattgaac agcctcaaag
      901 ggcaggatga acggattctc atcccaggtt tttacgatgg catcattccg cccaatgaac
      961 gcgaactcga attgatgaaa aagcttcccg atgttgccga tgaatacaaa aaacgttttg
     1021 gtgtaaagga attcatcaaa ggcatcaaag gcggcaacga actgaagatc gaagaagtgt
     1081 tcacccccac ctgtaccatc tgcggcatca ccagcggata tcagggcccg ggctcaaaaa
     1141 ccgtgcagcc tgcacgcgca tctgccaaag tggatttccg ccttgtgcct gcgcaaatgc
     1201 ctgaagatat cgtcaagaaa ctccgcgccc atcttgatgc ccagggtttc acagatgtaa
     1261 aaatcgactt cctcggcggc gagcctgccg cacgcacaaa ccccgacgat cctttcatta
     1321 aattgatcgt ggatactgcc gcggatattt acgactcgcc aatggaactt gtccccactg
     1381 tcggtggatc gggccccaat tacccgttcg tccatgacct tggcctgccc gttgcgacga
     1441 tgggcctggg tcaccccgac actcgtgccc acgcgccaaa tgaaaacatt cgccttgatt
     1501 tgtatttaaa acacgccaaa cacatggcac gggttgtgaa agaattttca aagtaacctc
     1561 tgaatttctg caacggaatt gacatgttcc tcaatcgctt atacaatgct cgtattcaga
     1621 ccaatttagt ttgaaattca tttttcgagg agaaacgaat gagta
//
LOCUS       DAOQLC010000354         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_55784_length_1652_cov_17.941140, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000354 DAOQLC010000000
VERSION     DAOQLC010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55784_length_1652_cov_17.941140"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1652)
                     /locus_tag="PL000_22610"
     CDS             complement(<1..>1652)
                     /locus_tag="PL000_22610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC91731.1"
                     /translation="GYFEIVSHYLLTDPAIKGIVINGHDITERKQAEIALQESEARWQ
                     FALEGPGDGVWDWNVESNQVFFSRRLKTMLGYDEAENWNALHEWDKRLHPDDRESAYQ
                     SLNLHFENKTPYYQSEHRLLCKDGTYKWILDRGKVTEWTEEQKPRRMIGTHTDITKRK
                     QVEQELKTSEERYRILYEDTPSMYFTADSEGRIISINKFGVEKLGYSEAELRDHPLLE
                     VFYPDDHTMIQEQFTTCLQNVGQMVQMETRKIRKDGSLLWVRESARAVYDAHEQLVVL
                     LTCDDITESKKIEAALRTSEEKSNALYRMVRLMTDNLPDLVWAKDMDGRYLFANKAIA
                     EKLLIATDTNEPLGQTDLYFAQRQRNAHPENPTWHTFGELCINSDAVIHASQQAQRFE
                     EFGNVKGEFLFLDVYKAPFLDENGNMIGTVGVGRDVTYEKKLAEEHRQAQQALAASEA
                     ELRALFSSMQDTVLVIKRDGTYSRIAPTRPGKFYIPPERVVGKHLTDFFPQPWVKVFQ
                     EVIEKVLTTKLTQHIEYSININGATPWFEASISPMGEDATLW"
BASE COUNT          324 a          391 c          465 g          472 t
ORIGIN      
        1 ccacagggtg gcatcttcac ccatcgggga gatggaggcc tcgaaccagg gagttgctcc
       61 attgatatta atcgagtatt cgatgtgttg ggtcagtttt gtggtgagta ccttttcgat
      121 tacttcctgg aagaccttaa cccatggttg ggggaagaaa tcggtgaggt gtttgccgac
      181 cactctttcg ggggggatgt agaattttcc cgggcgcgtg ggggcgatcc tgctgtaggt
      241 gccatcacgt ttaatgacga ggaccgtatc ctgcatggaa gagaacaggg cgcgcaattc
      301 cgcttctgag gcggcaagcg cctgttgggc ctggcgatgt tcttcggcca gttttttctc
      361 gtaggtcaca tcgcggccaa cgcccacggt gccgatcatg ttcccgtttt cgtcaaggaa
      421 gggggctttg tacacatcga gaaacaggaa ttcgcctttg acatttccaa actcttcgaa
      481 acgttgggcc tgctggctgg catggatgac ggcatctgag ttgatgcaca gttcaccgaa
      541 ggtgtgccag gtggggttct cggggtgggc atttctttgt cgctgggcaa aatacaggtc
      601 ggtttgaccg aggggttcat ttgtgtcggt tgcgatcagt aatttttcag cgatcgcctt
      661 gttggcgaag agataacgtc cgtccatatc cttggcccaa accagatctg ggaggttgtc
      721 ggtcatgagg cggaccattc gatacagggc gttcgatttt tcttcgcttg tgcgcagggc
      781 cgcttcgatc tttttgcttt ctgtgatgtc gtcacaggtg agcaggacaa ccagttgttc
      841 atgtgcatca tatacggcac gggctgattc tcgaacccat aaaagtgagc cgtctttccg
      901 tatctttctt gtttccatct gaaccatttg acccacattc tgcaggcagg ttgtaaattg
      961 ttcctgaatc atggtgtggt cgtcggggta aaaaacctca agcagaggat gatctcttag
     1021 ctcggcttcc gagtatccga gtttttcaac cccaaatttg ttgatggaaa tgatcctgcc
     1081 ttcagaatcg gccgtgaagt acatggaggg agtatcttca taaagtatgc ggtaacgctc
     1141 ctcactggtc ttgagttcct gttcgacctg tttgcgtttg gtgatgtcgg tatgcgtgcc
     1201 gatcatgcgt ctgggttttt gttcctctgt ccattctgtg actttgccgc ggtcgaggat
     1261 ccatttatag gtgccgtctt tacataacaa gcggtgttcg ctttgataat agggtgtttt
     1321 gttttcgaag tgcaggttga gggactggta ggcgctctca cggtcatcgg ggtgcagacg
     1381 cttatcccat tcgtgcaggg cattccagtt ttcagcctca tcatacccaa gcatggtctt
     1441 cagacgacgt gagaaaaaga cctgattgga ctccacgttc caatcccaaa ccccgtcgcc
     1501 ggggccttcc aatgcgaatt gccagcgtgc ttcactctct tgcagggcta tttcggcttg
     1561 cttgcgctcg gtgatgtcat gaccgttgat cacgatcccc ttgatggcgg ggtcggtaag
     1621 caggtagtga cttacaattt caaagtaacc cc
//
LOCUS       DAOQLC010000355         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_55785_length_1652_cov_17.022542, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000355 DAOQLC010000000
VERSION     DAOQLC010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55785_length_1652_cov_17.022542"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="PL000_22615"
     CDS             complement(<1..1543)
                     /locus_tag="PL000_22615"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNC91732.1"
                     /translation="MTDPHKKSQAYQFLSFGAIKPSGWIRTQMSRDLEHGFVGCLDEL
                     VPALIQNDDIYGADRLTKAARTKELGVVAKETQWEVQFLWWNSETQSNWLDGMLRTTL
                     LLDHPLYFEKARTHIERLLATQDTDGYLGIYAPDLRFNFTGENGELWAQASLFRVLLG
                     WYEATRDERALTAVRHAVAVTMQAYPMGQSRPFDVKDDFAGVGHGLVFTDILDRLYQL
                     TTDKQYLAYAHWFYEEYSRQPLSQDDIQIANLLDPNHRFKAHGVHTYEQLRSLLAATV
                     AAADSQLDSALAAALAKLETCLTPSGAPIGDEFIAGREADASETGYEYCSIHELLDTY
                     THLLQKTGDIRWADRAEWLFFNAAQGARHPSEPSIAYLKTDNSFSMTGSLHPDDPQDE
                     QNPQIRYKYSPVHQDVAVCCVPNAGRIAPYYIKAMWLRSEDGFVKALYGASVFQAEIN
                     GVSIKITETSNYPFDTNINFQVDVSEPIEFTLSFRKPEWSKQFKLTGADEFSVRGNLI
                     QVRKNW"
BASE COUNT          380 a          409 c          454 g          409 t
ORIGIN      
        1 gccagttttt tctcacctga atcagattcc cccgaacgga aaattcatcc gcgcctgtta
       61 atttgaattg cttgctccat tcaggttttc taaaactgag tgtgaattcg ataggctctg
      121 aaacatcaac ctgaaagtta atgttcgtat caaagggata attgcttgtt tcagtgatct
      181 tgatgctcac gccattgatc tcggcctgaa aaactgatgc gccatacaga gctttaacga
      241 aaccatcctc tgaacgcagc cacatggctt tgatgtaata gggcgcgatc cgccctgcat
      301 tgggaacgca gcataccgcc acatcctgat gtacgggtga atacttgtaa cgaatctgcg
      361 ggttttgctc atcctgcggg tcatcaggat gcagcgaacc agtcatcgaa aaggagttgt
      421 cggttttcag ataggcaatg gacggttcgg atggatgccg cgcgccctgg gcggcgttga
      481 agaacagcca ttcagcgcgg tctgcccagc gaatgtcgcc agttttctga agcaggtgag
      541 tatatgtatc gagcaattcg tggatcgaac agtactcata ccctgtctcg gaggcgtctg
      601 cttcgcgccc cgcaataaac tcatccccga tcggagcacc cgatggggtg aggcaggttt
      661 ccagtttggc aagcgcggcg gcaagtgcgg aatccagttg tgagtcagcg gcagcaactg
      721 tagcagccaa caatgaacgc agttgttcat aggtgtgaac gccgtgggct ttgaagcgat
      781 ggtttgggtc gagaaggttg gcgatctgga tgtcatcctg actgagcggc tggcggctat
      841 attcttcata aaaccagtgg gcatatgcaa ggtactgttt gtcagtggtg agttggtata
      901 agcggtcaag aatgtcggtg aacaccagcc catgcccgac cccagcgaaa tcgtctttca
      961 catcaaaggg acgtgactgc cccatggggt aggcctgcat ggtcactgcc actgcatggc
     1021 ggacggcagt gagtgcgcgt tcatccctgg tggcttcata ccagccaagc agaacacgaa
     1081 acaatgaggc ttgcgcccac agttcaccgt tctcgcctgt gaaattgaag cgcaggtcgg
     1141 gggcatagat tcccagataa ccatccgtgt cttgcgtggc gagaagtctt tcaatgtgag
     1201 tgcgcgcttt ttcaaaatag agtgggtgat ccaataacag ggtggttcgc agcatgccat
     1261 ccagccagtt ggattgggtt tcgctgttcc accacaggaa ttgcacttcc cattgagtct
     1321 ctttggcaac cacgccaagt tccttggttc ttgcggcttt cgtcaggcgg tctgcgccgt
     1381 agatatcatc gttttgaatc aaagcaggaa cgagttcatc caggcagcct acaaaaccat
     1441 gttcaaggtc gcgggacatc tgggtgcgaa tccaaccaga gggtttaatt gcaccaaaag
     1501 acaggaattg ataggcttgc gattttttat ggggatctgt catactctgg ctcccagatc
     1561 gcgcaatgcc tggctttgtg cttttagatt ttcgatgggt gtttcatctg ggatctcgca
     1621 cccgcccgcg gaaatccagc gggagccgcc ag
//
LOCUS       DAOQLC010000356         1641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_56492_length_1641_cov_26.500000, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000356 DAOQLC010000000
VERSION     DAOQLC010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56492_length_1641_cov_26.500000"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1641
                     /locus_tag="PL000_22620"
     CDS             <1..>1641
                     /locus_tag="PL000_22620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HNC91733.1"
                     /translation="NEGTLAISQNANLGKAETGVTVSNATLLLNGDIAIERKVTLAGT
                     AATIDTTGDSSIDDAVSGSAKLIKTGDGTLTLSTANTYAGGTQIAKGTLEISSDASLG
                     AKASKFGVDVVIDDAATLALTGNVAAARKLNLTGTTATIETTKEGSSSSLSEVISGSA
                     KLVKEGAGSLTLSGVNTYAGGTDIDEGTLVISQDGNLGKAGTGVVIGNEATLALNGNV
                     NVDRTLALAGTTATVNTVTGSSSLVKEVSGSATLVKTGAGTLTLNSANSYAGDTHIEG
                     GTLAINNDNKLGAGTAVTIKDATLALTESVALSRTLDVTGIATVDTAKDSNSSLDKAV
                     NGSGQLVKTGTGTLELTQANSYSGGTAIKEGNVTLAANAALGTGAATFAKDTELSLGI
                     SKGVLVANDVQVDGEVDIHSGIFDSSLTGVISGTGSLVKDGAGLLSLTGANSYAGGTQ
                     VNAGKVALDANTALGSGAATFAKDTELSLGISKGISVANAIEVNGEVDIHSGSFDSSL
                     TGVISGTGSLVKDGAGLLSLTGANSYAGGTQVTAGKVA"
BASE COUNT          498 a          296 c          431 g          416 t
ORIGIN      
        1 ttaatgaggg taccttagcc attagtcaaa atgccaactt aggtaaagca gaaacaggtg
       61 tgactgtaag taacgccacg ttattattaa atggtgatat tgccattgaa cgtaaagtga
      121 ccttagcagg tacagcggct acgattgata caacaggcga tagcagcata gatgatgcgg
      181 taagtgggtc ggccaagtta attaaaacag gtgacggcac cttaacctta agcacagcga
      241 atacgtatgc aggcggcact caaattgcca aaggcacctt agagattagc agcgatgcga
      301 gtttaggagc taaagcaagt aaatttggtg ttgacgttgt gattgatgat gcagctacct
      361 tagctttaac aggtaatgta gcggctgcgc gtaaattaaa tttaacaggc acaacggcca
      421 cgattgaaac cacgaaagaa ggtagtagca gcagtttaag tgaggtgatt agtggttctg
      481 cgaagttagt taaagaaggt gcaggtagct taacgttatc gggtgttaat acgtatgcag
      541 gtggtactga cattgacgaa ggtaccttag tcattagcca agatggcaac ttaggtaaag
      601 caggtacagg tgttgttatt ggcaatgaag ctaccttggc tttaaatggt aatgttaatg
      661 ttgatcgtac cctagcttta gcgggaacca cagcaacggt taataccgtg acaggtagca
      721 gcagcctagt caaagaggtc agtggttcag cgaccttagt taaaacaggt gcaggtacct
      781 taactctcaa tagtgccaat agctacgcag gtgacaccca cattgagggc ggcaccttag
      841 ccattaacaa tgataataaa ttaggtgcag gtaccgctgt taccatcaaa gatgccacct
      901 tagccttaac agagagtgtt gcgcttagtc gtacactcga cgtaacaggt attgccacgg
      961 ttgacactgc taaagacagc aacagcagct tagacaaagc agtcaatggg tcaggtcaac
     1021 tcgttaaaac aggtacaggc actttagaac ttacccaagc taatagttat agtggtggaa
     1081 cggcgattaa agagggtaac gttaccttag cagccaatgc agccttagga acgggtgcag
     1141 ccacgtttgc caaagatacc gaattaagtt taggtattag caaaggtgtc ttggttgcta
     1201 atgatgtaca agtagatggt gaagttgaca ttcatagtgg catctttgac agcagtttga
     1261 caggtgttat cagtggtaca ggtagcttag taaaagatgg tgctggctta ttaagtctga
     1321 caggtgctaa cagctacgca ggtggaacac aagttaacgc aggtaaagta gcgttagatg
     1381 ctaatacggc tttaggttca ggcgcagcca cgtttgccaa agataccgaa ttaagtttag
     1441 gtattagcaa aggtatctcg gttgccaatg ctattgaagt taatggtgaa gttgacattc
     1501 atagtggcag ctttgacagc agtttgacag gtgttatcag tggtacaggt agcttagtaa
     1561 aagatggtgc tggcttatta agtctgacag gtgctaacag ctacgcaggt ggtacacaag
     1621 ttactgcagg taaagtagcg t
//
LOCUS       DAOQLC010000357         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_57366_length_1628_cov_22.148125, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000357 DAOQLC010000000
VERSION     DAOQLC010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57366_length_1628_cov_22.148125"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PL000_22625"
     CDS             complement(<1..527)
                     /locus_tag="PL000_22625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC91734.1"
                     /translation="MDEKQAIQRLKQGDIGGLEFLVARYQVKAVRTAYLITRDAGLAE
                     DVVQDSFLQAFRAISGFDSTRAFEPWFLRSVVNATVKMMQRSARQVEAGDDADESIFA
                     ELALRVESVEEQVESIEFEDQIWDAMQKLSPRQRAVIVQRYFLEMSEKEMAEETGSAV
                     GTVKWMLNAARERLRG"
     gene            complement(608..1417)
                     /gene="surE"
                     /locus_tag="PL000_22630"
     CDS             complement(608..1417)
                     /gene="surE"
                     /locus_tag="PL000_22630"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC91735.1"
                     /translation="MKKKKPHILLTNDDGIRSPGLWAAASELSKIGYVTVAAPREQST
                     GMGRSLPSTSDGIIRKEQVQVNGQSWDVYAVGGSPAQAVLHGVLEIVPHKPDIIVSGI
                     NYGENVGLGITISGTVGAAMEAASMGFPALAISLQTEPEFHLSHSDEIDFTVAGYFTA
                     YFAKMILEREFPENVHLLKVEVPSHATPETEWQVGRLSRNRYYDPVPAKRGSWEQPGP
                     ISYKHSAGIDEDPEDTDAHILRTKKMVAVTPINLDMTAPVILSDFEKRLRS"
BASE COUNT          390 a          449 c          370 g          419 t
ORIGIN      
        1 ccgcgcaacc gttcacgcgc cgcattcagc atccatttca ccgtgccaac tgccgagcct
       61 gtctcctcgg ccatttcctt ttcactcatc tcaagaaaat atcgttgaac gatcaccgca
      121 cgttggcgtg gcgataattt ttgcatggca tcccagattt gatcttcaaa ctcgatggac
      181 tccacctgct cttcaaccga ctcgactctc aaagcgagtt ctgcaaaaat ggactcgtcc
      241 gcatcatccc ccgcctcaac ctgccgcgcc gacctttgca tcatcttcac cgtagcgttg
      301 accacgctcc gcaaaaacca cggctcgaat gcccgcgttg aatcaaatcc gcttatggcg
      361 cgaaacgcct gaaggaagga atcctgcacc acatcctctg ccagccctgc atcacgcgtg
      421 atcaggtagg ccgtgcgcac agccttcacc tgataccgcg ccaccagaaa ctccagcccg
      481 ccgatatcgc cctgtttaag tcgttgaata gcctgttttt cgtccatctc tataagatat
      541 atccgcccat gcctcaaaag gttgggtatc aaaaaaggga ccctgaaaca ggtccctttt
      601 ttgagtttta gcttcgtaat cttttttcaa aatcagacaa aatcaccggc gctgtcatat
      661 caaggttgat gggcgtgacc gccaccattt tcttcgtccg caaaatgtgg gcatcggtgt
      721 cttcggggtc ttcgtcaata ccagcactgt gtttatagga gatcggcccg ggctgttccc
      781 acgatcccct ttttgccggg acagggtcat agtagcggtt gcgggaaagc cttccaacct
      841 gccattcggt ttcaggggtg gcatggcttg gcacctccac tttcagcaga tgcacatttt
      901 caggaaattc cctttctagg atcatttttg caaaataggc ggtgaaatat ccagcaacag
      961 taaaatcgat ttcgtctgaa tgagaaagat gaaattcagg ttcggtttgg agtgaaattg
     1021 ccaatgcagg gaaacccatt gaggcggctt ccatggctgc tcccaccgtt cctgaaatcg
     1081 tgatccccag gccaacattc tcgccgtagt tgattccaga aacgatgatg tctggcttgt
     1141 gtggaacgat ctccaaaaca ccatgcaaga cagcctgcgc aggagaaccg cccaccgcat
     1201 acacatccca tgactgaccg ttaacttgaa cctgctcttt gcgaatgatc ccgtctgagg
     1261 tgctgggtaa actgcgcccc attcctgtgg attgttcacg cggtgcggct acggtcacat
     1321 agccgatctt ggaaagttca ctggcggcag cccataaacc gggagagcgg ataccatcgt
     1381 cattggtcag aaggatgtgt ggttttttct ttttcatggc ggtcattata aatagaaaag
     1441 gaatgctgcg gaaaaggaat tttgatttga tatgatgtgt caatatcaaa tcatagatct
     1501 gctgcttaag ccgccgggag catattttat gtcagacttt ttctccctgc aggacacgaa
     1561 tatcatccct cgatggggcg tgtgacctaa atagaactct tgcataagtc cattttttta
     1621 ccaccaag
//
LOCUS       DAOQLC010000358         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_57619_length_1624_cov_18.941364, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000358 DAOQLC010000000
VERSION     DAOQLC010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57619_length_1624_cov_18.941364"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PL000_22635"
     CDS             complement(<1..220)
                     /locus_tag="PL000_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC91736.1"
                     /translation="MTLNDQYQKWKEVTLKKSLDKFKERKDRFEYSSGIEVPRLSLPP
                     TDDSAYEEKLGFPGEYPYTRGVQPTMYRS"
     gene            438..1481
                     /locus_tag="PL000_22640"
     CDS             438..1481
                     /locus_tag="PL000_22640"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HNC91737.1"
                     /translation="MTSNLTANLTIQTAQSINEIDRQTWNALSTGLPFQSHRWYQFGE
                     RVMYDCKPTYLLAYHQNELIGRAVLWKISSEPLPLSPGIGRTITETTLRRRPLLICRS
                     PLSNASGLILPKNSLRVETRNALSEIALQTSRQGNCLALVFDFLDEGDSQGWEGDFVP
                     VQLSDPGTVMHSRWASLEEFLESGNKKDRQHFKRTLREAEKLGITIEKHSKVNDVEAA
                     LKLIRNVERHYKNSPNPWMRSLLENLEQVQGTWLEAKQNGNLVGCGALFDDNEAQLTT
                     ALGLAENIPYVYLLLTYASLEDAFQKKLPVLRWGSGAYDVKKSLGFELEENNHIVVSS
                     TSLIIRTLIRSLR"
BASE COUNT          452 a          406 c          377 g          389 t
ORIGIN      
        1 gtgaacggta catcgtcggt tgaacgccgc gggtgtaggg atattctccg gggaagccca
       61 gtttttcttc gtaagcagag tcatctgtcg gcgggagaga gaggcgcggc acttcaattc
      121 cagaagaata ctcaaaacga tccttgcgct ctttaaactt atccagggat tttttgagag
      181 tgacttcttt ccatttttga tattgatcat tgagggtcat gggtaagtcc tttttaacca
      241 cgaaattttg aggggtaaat ccatgctcaa gtataaatcc aacctaaaat tagctgtagg
      301 acaagtgaca tgctgacaca cgcaaaacat cccgatttca tattcatcca cttcagatgt
      361 aataactttc ataattgtta acgcagggtg tgtgtgcaaa tttacgaact ttccaaaaca
      421 ggcagtaaaa ttacagagtg accagcaatt tgacggcgaa ccttaccatt caaaccgcgc
      481 aaagtataaa tgaaatagac aggcaaacct ggaacgcgct ttcaactggg ctgccatttc
      541 aaagccaccg ctggtatcaa tttggcgagc gcgtgatgta cgattgcaag ccgacttacc
      601 tgctggctta tcatcaaaac gaactcattg gccgtgccgt tttatggaag ataagcagcg
      661 agccgcttcc actctcaccg ggaattggtc gcaccattac tgaaaccact ctgcgccgac
      721 ggcctctgct catttgccgt tctcctcttt ccaacgccag cggattgatc ctgccgaaga
      781 attccctgcg cgtcgaaacc cgaaatgcac tcagtgaaat cgctctacaa accagccgtc
      841 agggaaattg cctggcactc gttttcgatt ttcttgacga aggggacagc cagggctggg
      901 aaggtgattt cgtgcctgta caactctcag acccaggcac tgtgatgcac agccgctggg
      961 ccagccttga ggagtttctc gaaagcggca acaaaaaaga ccgtcaacac ttcaagcgga
     1021 ccctgcgtga agcggaaaag ctcggcatta cgatcgaaaa acacagcaag gtcaatgacg
     1081 ttgaagccgc gctcaaactc atccgcaatg tggaacgtca ctacaaaaat tcgcccaacc
     1141 cgtggatgcg aagcctgctc gaaaatcttg agcaggtgca gggaacctgg cttgaagcca
     1201 aacagaatgg aaatttagtc ggctgcggcg cactgtttga cgataatgaa gcacagttga
     1261 ccacagcgct tggtcttgca gaaaacatcc cttacgtgta tctgttgttg acctacgcca
     1321 gcctcgaaga tgcatttcaa aaaaagctcc ctgttttacg ctggggcagc ggcgcatacg
     1381 atgtgaagaa aagccttggc tttgaattgg aggaaaacaa ccacatcgtt gtttcaagca
     1441 ccagcctgat catacgaacc ctaattcgat cactgagata atttatgagt atccttattc
     1501 caaccctcgc agaattcatg caactcccca cagaagaagt ggcaaatatt gtacgatcca
     1561 aaggctcaaa agccattgtg ttccctttta acggtacgcg ccgctggttt ctactggaac
     1621 atgg
//
LOCUS       DAOQLC010000359         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_58074_length_1617_cov_17.566581, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000359 DAOQLC010000000
VERSION     DAOQLC010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58074_length_1617_cov_17.566581"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..488
                     /locus_tag="PL000_22645"
     CDS             114..488
                     /locus_tag="PL000_22645"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNC91738.1"
                     /translation="MKAATFSVKTKIRPVGFPPVAKTLPGAIERIVSELKPEKIILFG
                     SYAYGKPTPDSDVDLLVIMNTKAKEIDRYVKVSNLLYPRQFPVDIIVKTPKEIAAERK
                     KQGNFFMREILTKGKVLYERGK"
     gene            472..861
                     /locus_tag="PL000_22650"
     CDS             472..861
                     /locus_tag="PL000_22650"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNC91739.1"
                     /translation="MSAANNVLEWVSYAEEDLIMAKTALKRSKPLTTASCFHSQQCAE
                     KYFKAILVARNIEFPKTHDLLILNTLCANTGIITGFKITDLGRLSGYAVHTRYPGNQP
                     TPAAAREALEIANTIRQFARIFLGLKK"
     gene            966..1580
                     /locus_tag="PL000_22655"
     CDS             966..1580
                     /locus_tag="PL000_22655"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HNC91740.1"
                     /translation="MPATFPSPFPAHQGLVYPLARRWPNSYDVIALSVGAAMPDFVDI
                     VFGFLLNGYFRHWFAHGLIFIPFNVLGGLMLTWLIKNLFSKLFKLKTPVEPEKQKGKA
                     SRLKVWTFSVTVGVLSHLFFDLFSHDTNLLLYPWYVDAHWFPQWWYTPWFVIPPPLSM
                     GPAYSVGVHTLVWLVLTALGTFWFYQLVFQKIKSTSRDAISPRV"
BASE COUNT          448 a          375 c          349 g          445 t
ORIGIN      
        1 caagtgctac gcctgtgggt tttcggaatg ttagtgggtt gaaggttgca ggttgaaggt
       61 tttggggcgg acgaatgtcc gccctttgtt tttcaaatgg taaaattcaa atcatgaaag
      121 cagcaacatt ctcggtaaag acaaagattc gccctgtagg gtttccgcct gtagcaaaga
      181 ctctaccagg tgcgattgaa cgcattgtct ctgaactcaa gcctgagaaa atcatcctat
      241 tcggttccta cgcctacgga aagccaactc ccgatagcga tgtggatttg ctggttatca
      301 tgaacaccaa agcgaaagaa attgacaggt atgtcaaggt atcaaacctg ctttacccgc
      361 gccaatttcc agtggatatt atcgtaaaaa cgccaaagga aattgccgca gaaagaaaaa
      421 aacaggggaa ttttttcatg cgcgaaatat tgaccaaggg gaaggtgctg tatgagcgcg
      481 gcaaataatg tacttgagtg ggtcagctat gctgaagaag accttatcat ggcaaaaact
      541 gccctgaaac gaagcaaacc attgaccact gcttcctgtt ttcacagcca acaatgtgcc
      601 gaaaaatatt tcaaagcgat tcttgttgcg cgaaatatcg aatttcccaa aacccatgac
      661 ttgttgatcc taaacacatt gtgtgccaac acaggtatta ttacgggttt taagatcacc
      721 gatctcggca ggctatcagg atatgctgtt cacacccgct atcctggcaa tcaacccacc
      781 ccagccgcag cacgagaagc tcttgaaatc gcaaatacga tccgtcaatt tgcgcgaata
      841 tttttagggt tgaagaaata aaaccatcgc aagcgctgta tccgtagggg aaataaaaag
      901 ttgatttaaa agaaccacct tttactacag ataaaaaact tccccccttc aataggaaaa
      961 taaccatgcc tgccaccttc ccatctccat ttcctgctca tcaagggttg gtctacccat
     1021 tggcacggag gtggccaaat tcttatgatg tcatcgcctt gtcggtgggt gcagccatgc
     1081 ctgatttcgt ggatattgtt ttcggctttc tcctgaacgg gtatttccgt cactggtttg
     1141 cacatggact tatttttatc cccttcaatg ttttgggcgg tttaatgcta acctggttaa
     1201 taaaaaatct tttttcaaaa ttattcaaac tgaaaacacc agtcgaacca gaaaagcaga
     1261 aaggcaaggc gtccaggcta aaagtgtgga ccttttcagt gacagtcggg gttctttcac
     1321 atctgttttt tgatctgttc tctcacgata cgaacttatt attgtatcca tggtatgtgg
     1381 atgcccactg gtttccgcaa tggtggtaca caccctggtt cgttatccca cctccgctta
     1441 gcatggggcc agcgtattca gttggagtac acacactcgt atggttggtc cttaccgctc
     1501 ttgggacttt ttggttctat cagttggttt ttcaaaaaat aaaatccact agcagggatg
     1561 cgatttctcc acgggtataa tcactccatg cccaacacat ctcacccact gacacgg
//
LOCUS       DAOQLC010000360         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_59179_length_1601_cov_28.901035, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000360 DAOQLC010000000
VERSION     DAOQLC010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59179_length_1601_cov_28.901035"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..>1601)
                     /locus_tag="PL000_22660"
     CDS             complement(42..>1601)
                     /locus_tag="PL000_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91741.1"
                     /translation="AQAAAVAAVSRSYPAKNAGLLVNACTASTGFTFTGATGVSGAVD
                     STFPSPEGANSAYVVVPYASGSGYGRINLTQNMTWAATDSISFRILIENPGIHYLQCS
                     ILQASTGAELKRTVSSDGLYEAAGFGWYNVTWKLSDFTVTGSPNYGAVFSSFQFQFGL
                     AGAGGGQGRVALSNIRKNASSVSLIVFCQDRGYDDVWTVRNYFAASGIPLTVYVDTTT
                     LDTAGFLTTAQSQSLYSDSSGLFEIASYPGFLPSLAHTSTGICLSQAVAGAGNLTING
                     SLTSAGTANLGAARKVVISTAGNNRTVSFTITGTLSGNAVSENVMGTWVSGDLVESVK
                     YYDTVTQIAVSAACSGNVTVGTSYSVAEHVAAMNAQISGMTAKGLLGSHLHFAYAGGQ
                     VSQPLASALASVGALTGRPNVHTQYVPRNMLLQDRSWNPLLLSATNAQQSLATLQSMV
                     DDIVTRGGLLVIYFHHVLTANDGNNPSYEDLATLISYVKTYQEQGKVRCITLKKLLGI
                     LDTIPHVDCVP"
BASE COUNT          387 a          453 c          399 g          362 t
ORIGIN      
        1 gactaggcac agggctcctt taatgcctat cacgtcttcc atcacggcac acaatcaacg
       61 tgcgggattg tatccaaaat ccccagaagt tttttcaatg taatacatct aacctttcct
      121 tgctcctgat acgtcttcac gtaggaaatc agcgtcgcaa gatcttcgta agacgggttg
      181 tttccatcgt tggccgtcag cacatggtgg aaatagatga ccagcaggcc tccgcgagtg
      241 acaatatcgt ccaccatgga ttgcagcgtc gccagagatt gctgcgcatt ggtcgcagac
      301 agcagcaacg gattccagct acgatcctgc agaagcatat ttctcggaac atattgtgta
      361 tgcacattgg ggcggcctgt aagcgcgcct accgatgcca gcgctgatgc gagaggctgg
      421 ctaacttggc ccccggcata tgcaaaatga agatggctgc cgagcaatcc ctttgccgtc
      481 atgccggata tttgcgcatt catcgccgca acatgctccg ccacgctgta ggatgtgccg
      541 acggtaacat tacccgagca cgccgccgac acggcgatct gagtcaccgt gtcgtaatat
      601 ttcaccgact ctaccaaatc gcctgacacc cacgttccca tgacgttctc ggaaaccgcg
      661 ttcccagaga gagtgcccgt gatggtgaat gacaccgtgc gattattgcc tgcggtcgaa
      721 atgacgactt tccttgctgc tccaaggttt gctgttccgg cgctggtgag actgccattt
      781 atcgtcagat ttccagcccc cgctactgcc tgtgacagac aaattccagt cgaggtgtgc
      841 gccaatgacg gcaagaatcc tggataggat gcaatctcga acagcccaga cgaatccgaa
      901 taaagtgact gcgactgcgc agtagtcaaa aacccggctg tgtctagcgt cgtcgtgtcg
      961 acatatacgg ttagaggtat tcccgatgca gcaaaataat ttctcactgt ccacacgtcg
     1021 tcgtatccgc gatcttggca aaacacgatt aacgatacag acgacgcgtt tttgcggata
     1081 ttcgacaagg caacgcgccc ttgtcctccg ccagcacctg caagtccgaa ctgaaactgg
     1141 aacgacgaaa atactgctcc gtagttggga ctaccagtaa ccgtgaagtc ggacagcttc
     1201 catgtgacgt tgtaccagcc aaaccctgca gcctcataca ggccgtccga cgataccgtt
     1261 ctcttcagct ctgcccccgt gcttgcctgc aaaatggagc attggaggta gtgaatcccc
     1321 ggattttcta tcaggatgcg gaaagatatg gaatccgttg cggcccacgt catattctgc
     1381 gtcagattga ttcgcccata cccagatccc gaagcatagg gtacaaccac atatgctgag
     1441 ttcgcccctt ccggactcgg aaatgtggaa tcgactgcac cagatacacc tgtagctccg
     1501 gtgaaagtga atccagttga cgcggtgcac gcattaacaa gaagtcctgc atttttggca
     1561 ggataggaac gggaaactgc cgcaacggct gccgcctggg c
//
LOCUS       DAOQLC010000361         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_59529_length_1596_cov_28.579494, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000361 DAOQLC010000000
VERSION     DAOQLC010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59529_length_1596_cov_28.579494"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1320
                     /locus_tag="PL000_22665"
     CDS             256..1320
                     /locus_tag="PL000_22665"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNC91742.1"
                     /translation="MKKHTQELSKDLANAKFAGIDFHKRTSVVAIGDTHGGLIQQVTL
                     INEERIIREYFQGFVGLECVIESCRGYEWLVELLEELGHTVHMGDSRSIKLIAVTRCK
                     TDKIDSKILMELLAKQFLPTCYQPSATERLLRELVRHRAQLVRTSVKHKLRVHANLAK
                     ENKCIYAPFSLKGRQQLKEVKLSEPRRQKVDDDLEVIDFLELKIAEQDAKVKYVARSN
                     PDVALLKTIPGFDVLTSIAFIAEVGDISRFRKANQVAAFLGLAPRVYSSGDTRRNGRI
                     TKCGSKLMRWMLVQSAWSAIRGSSNLRACYSQIGRRRGQKTAIIAIARKLATIAYHVL
                     KEKAEFDERMLDAGLDRAVF"
     gene            complement(1339..>1596)
                     /locus_tag="PL000_22670"
     CDS             complement(1339..>1596)
                     /locus_tag="PL000_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91743.1"
                     /translation="SMGTVGTVNTPESRMISSPFATFCTFWQHKVRVQCDVDSLGAEE
                     STGTLADVRDIQVAELTFFPFALQVRPGCLGGFRAGLTQFF"
BASE COUNT          398 a          457 c          397 g          344 t
ORIGIN      
        1 aaaaaatggt atggggacgg ttcagcgaac cggggcgcac gcccaactcc ctgccaggca
       61 aaagagccta ctgctcgcag ccctccgtca agcctcgctt cgcttggatg gcttgactgc
      121 gggacccctg cttcgccgta ttccggcagt gccgcatggg ggtgggcgtg ctcaatgctt
      181 tccggccggg caggtagcaa taaacacaag ggggctgtac cccaacgcac tctacccgga
      241 cggaggtttt tcaccatgaa aaaacatacc caggagttaa gcaaagatct cgctaatgcc
      301 aaattcgccg gcatcgattt tcacaaacgt acctcagtag tcgcaattgg cgatacccat
      361 ggtggtctca ttcagcaagt taccctcatc aacgaagagc gaatcattcg cgagtacttc
      421 cagggcttcg ttggtcttga atgcgtcatc gagagctgcc ggggctacga atggcttgta
      481 gagcttctgg aggagctcgg gcacaccgtc catatgggcg actcccgttc catcaagctc
      541 atcgccgtca cccgctgcaa gactgacaaa atcgacagca agatactcat ggaacttctg
      601 gccaagcagt tcttgcctac atgctaccag ccgtctgcca ctgagcgcct ccttcgcgaa
      661 cttgttcgcc accgagccca gctcgttcgc acttccgtca agcacaaact ccgtgtccat
      721 gccaatctgg ccaaggagaa caagtgcatc tacgcaccat tttcccttaa aggtcgacaa
      781 cagctgaaag aggtaaaatt gagtgagcct cggcgccaaa aggttgatga cgacctggaa
      841 gtcatcgact tcctcgaact caaaatcgcc gaacaagatg ctaaggtcaa gtatgttgct
      901 cggtcaaatc ccgatgtggc cttgctcaaa accatcccag gctttgatgt actcacgtcc
      961 atcgctttca tcgctgaagt cggcgatatc tctcgctttc ggaaagccaa tcaagttgcc
     1021 gcctttctag ggctagctcc cagggtctac tccagtggag acaccaggcg caacggccgt
     1081 atcactaaat gcggctccaa attgatgcgc tggatgctcg ttcagtccgc ctggtcagct
     1141 attcgcggca gctctaacct gagagcttgt tactctcaaa tcggtcggcg acgcggccaa
     1201 aaaaccgcca tcatcgccat tgccaggaaa ctcgccacta tcgcgtatca cgttttaaag
     1261 gagaaagcgg agttcgatga acgaatgctg gacgctgggc tagatcgtgc cgtcttttga
     1321 ccctgcctgc agaggtcgtt aaaaaaattg agtcagccca gcacgaaaac caccgaggca
     1381 accaggtcta acctggagtg cgaatggaaa aaatgtgagt tcagctacct ggatatcacg
     1441 cacgtcggct aaagttccgg tgctttcttc ggcaccaagt gagtccacat cacattggac
     1501 cctcactttg tgctgccaaa aagtacaaaa agtagcaaaa ggagatgaaa tcatgcggct
     1561 ttcaggggta ttgacagttc caaccgtccc catgga
//
LOCUS       DAOQLC010000362         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_60031_length_1589_cov_15.771186, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000362 DAOQLC010000000
VERSION     DAOQLC010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60031_length_1589_cov_15.771186"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1222
                     /locus_tag="PL000_22675"
     CDS             join(93..357,357..1222)
                     /locus_tag="PL000_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC91744.1"
                     /translation="MKKSRKKFTAEFKAKVALAAVREEGTLAELAAKYEVHPNQISQW
                     KKMLLDNASAAFEGGNSPEAAISEAAITELHAKIGELTVERDFLFQSAQALSKKERQT
                     LLDREVEAPSVRKQCELLSLNRSTLYYKAVEPAAETLELMKMIDEIFMDRPYFGARRI
                     RESLKRAGKQVTRKRVRRLMKLMGIEAVYRKPRTSKANPEHKVFPYLLKGLKIERPNQ
                     VFAADITYIPMAKGFVYLVAVIDWNSRYILSWRLSNSLETGFCKEALEDALAQATPEI
                     FNTDQGSQFTSDDFVKPVLASGAEMSMDGKGRYLDNIFVERFWRSLKYEEVYLHAYES
                     TKQARESIARWINFYNHQRPHQALDYRTPAEVHFGIKQEAIA"
BASE COUNT          450 a          371 c          449 g          319 t
ORIGIN      
        1 cagagctgga tcccaaaatt caggccagtg actaaggtgt aattcgctac cccataaatc
       61 gtataatcga gttaacaagg agtagcaggg ccatgaaaaa atcaaggaag aagtttacag
      121 ccgagtttaa ggccaaggta gcgctggcag ccgtgcgcga ggagggaacc ttggctgaat
      181 tagcggcgaa gtatgaagtg caccccaacc agatctcaca gtggaagaag atgctattgg
      241 acaatgcgag cgcagccttt gagggcggga atagcccgga ggcggctatc agcgaagcag
      301 caatcacgga actgcatgca aagatcggcg aattgacggt ggagcgggat tttttatcca
      361 aagcgctcaa gcgttaagca agaaggagcg ccaaactctg ttggacaggg aggtggaagc
      421 cccgtcagta aggaagcaat gcgagttgct ttctttgaac cgctcaacgc tttattacaa
      481 agcggtagag ccggccgcgg agacgctaga actgatgaag atgatcgacg aaatattcat
      541 ggaccgcccg tacttcgggg cccggcgtat cagagagagc ctgaaacgcg ccggcaagca
      601 ggtgacgcgc aagcgagtgc ggcgcttgat gaagctgatg ggaatagaag ctgtgtatcg
      661 gaaaccgcga acgagtaaag caaatcctga acacaaggtc ttcccatact tgttaaaggg
      721 cttgaagatt gagcgaccga accaggtttt tgccgcagac attacctaca ttccgatggc
      781 aaagggattc gtgtacttgg tagccgtaat cgactggaat agccggtaca tactgtcttg
      841 gcgactatct aactctcttg agacagggtt ctgcaaggaa gccttggagg acgcgctagc
      901 acaggccaca ccagagattt ttaacacgga ccagggcagc cagtttacga gcgatgactt
      961 tgtgaaacca gtgttggcct ccggtgctga aatgagcatg gacgggaagg ggcggtattt
     1021 ggacaacatt tttgttgaga ggttctggcg cagcctaaag tacgaagaag tctacttaca
     1081 tgcgtacgaa agtaccaagc aagcaaggga atcaatcgca cggtggatta acttctacaa
     1141 ccaccagaga ccacaccaag ccctggacta ccgaactcca gcggaggtgc actttggaat
     1201 caagcaagag gcaatagctt gatgaacatg aagacagacc tatttatggt gcttgacaca
     1261 ctacgttgtc gaactctccg gagcgacggc gtgcaactca cggcaggaca acagcgtggc
     1321 tggacgcacg ccgccgctgc cggtcagtct aacttcgtgg tcaagcacag actgcccttt
     1381 cagggtgggt taggtacgcc tggcggcgcc tccttcctgt gggagccgcc agcgccaaac
     1441 tacagcattt tcaaaagacc gggaggagaa ctcagcaaga aattaaggaa agacaattcg
     1501 tacgctgata ctattagtgg tatcattgat atatcaaagg tgagcagcga atttccacct
     1561 taactctcta agtttgtggc ctaaacaat
//
LOCUS       DAOQLC010000363         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_60261_length_1586_cov_22.111039, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000363 DAOQLC010000000
VERSION     DAOQLC010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60261_length_1586_cov_22.111039"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            788..1081
                     /locus_tag="PL000_22680"
     CDS             788..1081
                     /locus_tag="PL000_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91745.1"
                     /translation="MTTARTLPVPTTGEGRLIEHFGDSVVQSTLRERVGPSEFYRAVG
                     YLSTWNMTYPHVKLYYNARELEITASYYDAFGTLGYCIGAVFNDTSKQFGFHS"
     gene            1225..>1586
                     /locus_tag="PL000_22685"
     CDS             1225..>1586
                     /locus_tag="PL000_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91746.1"
                     /translation="MIRIARAIRRILDSRAETKTLALLEAEDAILTELIKQHRLLNRQ
                     RMARIRMGAKHIGHPDYRFNGRHSKKKLEGEAYSTPRSWHMAANLVEAKPPFDVMMDM
                     LNTFEEHCKKKLEGEAYST"
BASE COUNT          380 a          460 c          381 g          365 t
ORIGIN      
        1 gtgtcaaatc tctatacaat tatgcggttt agataatagc cccatttgta agccgttgat
       61 tttccacaca ataggcagaa atttgggcgt ttgcctacca tttgcctact tttcccaaag
      121 ggcgggcagg cgctaaaggg ttgaaatata tgtgttttcc ttattacaca taaataaata
      181 ggcaaatagg cagactaggc agcgtttata gatgttcagt gcgcgaggcc cgctcagtgt
      241 aaagcggtta tacacataat gtataacaat taaacagtat aacaatttga cactaaccac
      301 gtgcccgccc aatcccatac gtaaagacct agaaaatgct gcctattctg cctattctgc
      361 ctattcgctg gataatcaac cacttagcct gcctatttat gcgtttaaac taggcaacac
      421 ctcgcatttc tgcctatttt tctgtcgaaa ccggcacaat ctgtcgattt acacttggca
      481 gcccgtttat ttacccgcaa atacaaactg caacggccat ttccgcgcct cgtatattga
      541 cttatatgtc aaatgcgggt acaatgtgta cagactcgta acaaagcgag tccggcccgc
      601 cccgacgtcg tggggcattt ggtaactacc aaaacggagg tttctactat gtatgatgat
      661 ttgacaccgc gcctttgggc gttcgctgta aagcggttga acaacattgc ccgcacgatg
      721 gcccgcaccg cgtaccgtgc tggcctgccg attgagttgg cgttgcaagc gtgtaaagga
      781 gtttgacatg acgaccgccc gcacattgcc tgtccccacc actggcgaag gccggctgat
      841 cgagcacttc ggcgacagcg tggtgcaaag cacgctgcgt gagcgcgtcg gcccgtccga
      901 gttctaccgc gccgttgggt atctgagcac gtggaacatg acctatccgc acgtcaagct
      961 ctactacaat gcccgcgagc ttgagatcac ggcgagctac tacgacgcgt tcggcacgtt
     1021 gggctactgc atcggcgctg tgttcaacga cacgtccaag cagttcgggt ttcactcgta
     1081 gcataatggg cgcactcttc tttttggtaa ctaccaaacc agccatgtgc ccaatcgtcc
     1141 tgatcctcat cgtgtggctg gcccttggcc tgctgccaac cttgttcatc gcaccgtctg
     1201 aaccatctga aaaggagtaa caccatgatc cgcatcgccc gcgctatccg ccgtatcctt
     1261 gactcccgcg ccgagaccaa gacactcgcc ttgctcgaag ccgaggacgc cattctcacc
     1321 gaactcatca agcagcatcg cctgctcaac cggcagcgca tggcgcgcat ccgcatgggg
     1381 gccaagcaca tcggccaccc ggactaccgc ttcaacgggc ggcactccaa gaagaagctc
     1441 gaaggtgaag cgtactccac gcctcgttcg tggcacatgg cagccaacct cgtcgaagcc
     1501 aagcctccgt tcgacgtgat gatggacatg ctcaacacgt tcgaggagca ctgcaagaag
     1561 aagctcgaag gtgaagcgta ctccac
//
LOCUS       DAOQLC010000364         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_60420_length_1584_cov_13.888162, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000364 DAOQLC010000000
VERSION     DAOQLC010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60420_length_1584_cov_13.888162"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1289
                     /locus_tag="PL000_22690"
     CDS             117..1289
                     /locus_tag="PL000_22690"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNC91747.1"
                     /translation="MPIKKVITGGRVPVKVWSENIEDKALEQLKNTASLPFVFKHVAA
                     MPDVHLGVGATIGSVVATKGAISPAAVGVDIGCGMMAVKTNLDINRVQDKIKEIRHSI
                     ERSIPVGFDGNRKLSPTVEAWEGWGRWSHERDGLFKRSQEQLGSLGGGNHFIEICRDT
                     EGNCWVMLHSGSRGVGNVLAARHIEKAKGLMKQMFISLPDPDLAYFVVGTKDFNEYVA
                     DLDWCQGYAFENRQEMMRRVLKDVSFALFGEDGKVERRLEVNCHHNYMAWENHFNENV
                     MVTRKGAVRARMGDYGIIPGSMGTKSFIVRGLGNTESFNSCSHGAGRRMSRAQARKQF
                     TTTDLENQTATVECRKDAGIIDEIPSAYKDIDDVMLNQSDLVSVEAMLKQIMCVKG"
     gene            1388..1558
                     /locus_tag="PL000_22695"
     CDS             1388..1558
                     /locus_tag="PL000_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91748.1"
                     /translation="MAEPKRVTLAMANVTVDVPEENVDLVFTVGTPCSMTCDDNCREM
                     QEQFEAMVEGWA"
BASE COUNT          417 a          320 c          504 g          343 t
ORIGIN      
        1 cgaagccaca gaaggtcgag atggcggtgg ccgagatcga ggagaagttg gggatggaga
       61 agggaacgct ggcggtgaag gcgtgaaaaa attacgagaa aatgggggtt taaataatgc
      121 cgattaaaaa ggttatcacc ggaggaagag tcccggttaa ggtttggtcg gaaaacattg
      181 aagacaaggc gttggagcaa ttgaagaaca cggcaagtct tccgtttgtg tttaagcacg
      241 tagcggcgat gccggacgtt catctcgggg tgggtgcaac gattgggtct gtggttgcca
      301 ccaagggtgc cattagtccg gcggcggttg gagttgacat tgggtgcggg atgatggcgg
      361 taaagacgaa tcttgacata aaccgtgtcc aagacaaaat caaagaaatc cgacattcca
      421 tcgagcgcag catcccggta gggttcgacg ggaacagaaa gctttccccg accgtagaag
      481 cttgggaggg ttgggggcga tggtcgcacg aaagggacgg tctttttaag cgttcccaag
      541 aacaattggg ttcactggga ggcggtaatc attttatcga aatctgccgt gacacggaag
      601 gaaactgttg ggttatgctc cacagtggtt ctcgcggggt tggtaacgtt ctggcggccc
      661 gccacatcga aaaagcgaag ggtctgatga agcagatgtt catctctttg ccagacccag
      721 acctggccta ttttgtcgtc ggaacaaaag atttcaacga atacgtggcg gaccttgatt
      781 ggtgtcaggg gtatgccttc gagaaccgac aggagatgat gcggcgcgtc ttgaaagatg
      841 tttcgtttgc tctattcggg gaggatggca aagttgaacg tcgtcttgaa gtaaactgcc
      901 accacaacta catggcgtgg gagaaccatt ttaacgaaaa cgtgatggtt acacgaaaag
      961 gggcagttcg cgccagaatg ggcgactacg gaatcatccc tgggtcgatg ggaacaaagt
     1021 cgttcatcgt caggggtttg gggaacacgg agtcatttaa ttcatgctca catggtgcgg
     1081 gtcggaggat gtctcgtgcg caagcccgca agcagttcac gacgacggac cttgaaaatc
     1141 agacggcgac ggtggagtgc cgtaaagacg ccgggattat cgacgaaata ccttcggcct
     1201 acaaggatat tgacgacgtg atgttaaatc aatcggactt ggtttctgtg gaagccatgc
     1261 tcaagcagat catgtgcgta aagggctgat gacggaggat ttgtcggtaa ctgcatagga
     1321 ctgtttaatt ccaggcccgc caaatgacag ccgggaaaga ccggcaacga tttgaggagg
     1381 acgacgcatg gcagaaccta agcgagtgac attggcaatg gcaaacgtca ccgtcgatgt
     1441 tccagaagaa aatgttgatc ttgtgttcac ggtggggacg ccgtgttcta tgacttgcga
     1501 tgataactgc cgtgaaatgc aggaacaatt tgaggctatg gttgaagggt gggcgtagcc
     1561 atgaccacgg agggcgcgaa gatg
//
LOCUS       DAOQLC010000365         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_60507_length_1583_cov_26.280105, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000365 DAOQLC010000000
VERSION     DAOQLC010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60507_length_1583_cov_26.280105"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..74
                     /locus_tag="PL000_22700"
     tRNA            3..74
                     /locus_tag="PL000_22700"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Met,seq:cat)
     gene            783..1532
                     /locus_tag="PL000_22705"
     CDS             783..1532
                     /locus_tag="PL000_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91749.1"
                     /translation="MEEAERLYADFQVKIAGDEVWRPNDLEFLHASITILSNAMGGSE
                     NFKQQLGEVLIERTDTGKHLGLAHRGRIQLSKSTPFSAWSVIHELSHAWDAKHHWMLS
                     VLLQKYTGGFTSRFLSRIKKWFPRQWDAGRHGAEQTPGRYGRKPGVNAFGYFYGDRPS
                     GSNWRFNHKEDFAESLVMYCGWGKNNLLSRTAHGRIERYTLPNGERDPLYGIVDQWSD
                     YARYFYPPEGDYAQTKRWKFFDELIHGKIPT"
BASE COUNT          416 a          421 c          391 g          355 t
ORIGIN      
        1 acgccgaggt agctcagtgg tagagcaggg gactcataag cccttggtcg ggagtccaaa
       61 tctccccctc ggcactcgaa aacccgtgca aacgggtttt tttttcttta tcatgcagat
      121 tcaacccgat acactcatcc gcagcaggcg cagaacgatc gctttgatca tcgagcgtga
      181 cggcacactc accgtccgtg cgccgatgcg tgccagcaag gcggtcattg aggaattcat
      241 tcgcgaaaaa gcggattgga tcacccgcac acgcaaaaag ctgactccca tcctaaccgc
      301 cccggagaag caatttgtcg acggggaaaa cttcctcttc ctcggttcct cctttgactt
      361 gaaactggtc cagcgacagc ggccagccct gcgctttgag aacggattca cgctcagcat
      421 cgccgcgcaa aaaagaggca ggcaggtctt caccaactgg tacaaggaac gtgcgcttga
      481 agtcatcacg gaacgcgtca atcatttttc cgcaagatat gggtttcaac ccaaacaggt
      541 gaaaatctct tctgcaaaaa cacgctgggg atcgtgcagc gccaacggga cgttgaattt
      601 cacatggagg ctggtcctct ctccgttaag tgtgatcgat tatgtcgtcg tgcatgagct
      661 tgcccatttg cgggtccgaa atcattcgcg caaattctgg caggtcgtcg aatccattga
      721 ccctgattac aaaaccaaaa gaaaatggtt gcgcgaatac ggagaaacgt tatacttcta
      781 acatggaaga agctgaacgc ctgtacgccg attttcaggt caagatcgcg ggggatgaag
      841 tttggcggcc caatgacctg gaattccttc atgcctccat cacgatcctt tcaaacgcaa
      901 tgggtggcag tgaaaatttc aaacagcagc ttggggaagt tctgattgaa cggaccgaca
      961 caggcaaaca tctcgggctg gcccaccgtg gacgaatcca attaagtaag agcactccct
     1021 tctcagcctg gtctgtaatt catgaactct cccatgcctg ggatgcaaaa catcactgga
     1081 tgctttccgt gttgctgcaa aaatacacag gcggatttac ctcccgcttt ttatcgcgca
     1141 tcaaaaaatg gttcccccgg caatgggatg caggcagaca tggcgctgag caaaccccgg
     1201 gccgctatgg aaggaagccc ggcgtgaacg cttttggata tttttatggc gatcgtccca
     1261 gcggctcgaa ctggcgcttc aaccataaag aggattttgc cgaatcgttg gtgatgtact
     1321 gtggctgggg aaaaaacaac ctgctttcgc gcaccgccca cggacggatc gaacgctata
     1381 ccctgcccaa cggtgaacgg gatccgctct atggcattgt ggatcaatgg tcagattatg
     1441 cccgttattt ctacccgcca gaaggcgact acgcccaaac caaacgctgg aaattttttg
     1501 acgagttaat tcacggaaaa atcccaactt aatgaataga tctcttgcaa aagattaaag
     1561 tcttaaccac aaagatcatg gaa
//
LOCUS       DAOQLC010000366         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_60762_length_1580_cov_16.830164, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000366 DAOQLC010000000
VERSION     DAOQLC010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60762_length_1580_cov_16.830164"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..394
                     /locus_tag="PL000_22710"
     CDS             212..394
                     /locus_tag="PL000_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91750.1"
                     /translation="MNLHQKIAAHLAKISEEARQDYASRQIDEVMTDALYRDCDADTK
                     EEMNEALEIAIELCRK"
     gene            636..971
                     /locus_tag="PL000_22715"
     CDS             636..971
                     /locus_tag="PL000_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91751.1"
                     /translation="MKTPKSKAERLAPVTGSPSLQLALAELADWAMIEAKAADSTVAQ
                     NIWLQVRDWSSNGALSGKVPKAKHPLPPDTDILEALEARVPGIIATGARWARKANDAD
                     QATASQAHG"
     gene            1134..1382
                     /locus_tag="PL000_22720"
     CDS             1134..1382
                     /locus_tag="PL000_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91752.1"
                     /translation="MSTQFKVTLRKRTQGTWDEDYSSGHLGFMAEYFDSKAEAEAEAD
                     KRNEKLAQDMADGCGMDVEEWNETRAQDCDKYWHVIEA"
     gene            1384..1575
                     /locus_tag="PL000_22725"
     CDS             1384..1575
                     /locus_tag="PL000_22725"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC91753.1"
                     /translation="MTFAEQLKAHRQRLGLTQSEAAALLDVPARTYWEWESGKTTPHA
                     ITQEGAIARLSKAKRPPKE"
BASE COUNT          432 a          430 c          433 g          285 t
ORIGIN      
        1 gaaccatgac ggggggcgcg tgtcgcgtgt gctcgttatg aattgcccga acgactaagc
       61 tctgccacga gcggagcgag ttgggcagca gcgtattgtt ctggcccgtt tccatcctcc
      121 gaaaaatatt ttcacatttc gcttgcgcta atacccgatt cgggtattat ctcaatcgtc
      181 agcaatgagg ctggcaccaa caaaaaacat tatgaatctt caccagaaaa tcgccgccca
      241 ccttgccaaa atctccgaag aagctcgtca agattacgca tctcgtcaaa ttgacgaggt
      301 gatgactgat gcgctgtatc gcgattgcga tgcagacacc aaagaggaga tgaacgaagc
      361 cttggaaata gctattgagc tgtgccgcaa atgaccttcg ccgaacaact ccgagccgcc
      421 aaaggcgacc tgaccgcaag gcaggtcgcc gttgccgttt cgccgttgct ctctgtgagg
      481 acggtggaga agtggttggc tggcgacaat gagccgcccg cgtggactca cgaatggatc
      541 attagcagtg tgcgaggaga actcacacca aggaaacggc cccgaaaaca gaagggctag
      601 aacactcaaa ctgtgccgac cgcgaagtga agactatgaa gacaccaaag agcaaagctg
      661 agcggttggc accagtgact ggttcgccat cgcttcagtt ggccctagca gaacttgcag
      721 actgggccat gatcgaagcg aaagccgcag actctacagt cgcacaaaat atctggctgc
      781 aagtccgcga ctggtcgagc aacggggcgc tctctggaaa agtgcccaaa gcaaaacatc
      841 cgctgccacc agatactgac attcttgaag cactagaggc acgcgtcccc ggcatcatcg
      901 caaccggtgc acgctgggca cggaaggcga acgatgcgga tcaggcgacg gcgagccagg
      961 cgcatggatg acacgacaga cgaccttcga gccgttgcct gcatcccgct ggttctcatt
     1021 ccgtggtctg acggctccaa aaagaaatcg caaaatctgc gaaatatttg ttgcatcagt
     1081 cgcggaatct gcgaatatgg cggggtcaga gaaacaacga accaaattca aatatgagca
     1141 ctcaattcaa agtcaccctt cgcaaacgca ctcaaggaac atgggatgaa gattacagca
     1201 gcggccacct cggcttcatg gctgaatact tcgactcgaa ggccgaagcc gaagccgaag
     1261 ctgataagcg caacgaaaaa ctggctcagg acatggcgga tggatgcggg atggatgtcg
     1321 aagaatggaa tgagactcgc gcccaagact gtgataaata ctggcatgtg atcgaagcct
     1381 aaaatgacct tcgctgaaca actcaaagcc caccgccagc gcctcggact cacgcagtct
     1441 gaggccgctg ccttgttgga cgtgcccgcc cgcacttatt gggaatggga gtctggcaag
     1501 acgactcccc acgccatcac gcaagagggg gccattgcaa ggctatcgaa ggccaaacga
     1561 cccccgaagg aatgagaacg
//
LOCUS       DAOQLC010000367         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_61105_length_1575_cov_19.842105, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000367 DAOQLC010000000
VERSION     DAOQLC010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61105_length_1575_cov_19.842105"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..646
                     /locus_tag="PL000_22730"
     CDS             194..646
                     /locus_tag="PL000_22730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNC91754.1"
                     /translation="MVVSFFFIMKRSFRHVGYIVRDLKQSVADFQRLFDLQDEDIRII
                     PPFELPADTRFAFFHADGLTFELIEPISEHFKNILFNSGVGINHICFTVDDIEAAVND
                     MAAKGIRPGHVTPNGIITMPHQKMVYFDPKDTGGVLIEYIEELPMNRK"
     gene            651..1427
                     /locus_tag="PL000_22735"
     CDS             651..1427
                     /locus_tag="PL000_22735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91755.1"
                     /translation="MEQKSINILGNSVTYWQGGDGAPLFLIHGGIGNAEVHWSPVWNQ
                     LTERYMVIAPNLPSFGETSPLERAGFAQIADWVITLMDALNVESANVIGNSFGGGVSR
                     LMASRHERRVKRLILVNGGAIPNIPPFVGRMMQSSIFNGMFNMIRKQTFSFKGLAPMF
                     ADKSFLTNEFIAQAEADSLGFVTLMRQSASEGVGTEVVPQVPTLVLWGEDDKFTKISM
                     GQNLAKMIPHAEFKLFTGTGHMPQIEKPDEFVEKVVEFLG"
BASE COUNT          376 a          356 c          428 g          415 t
ORIGIN      
        1 ttggcaaacc tgtcattgcg atcaacgccg ccacgtattg gatggctctg cgcgataatg
       61 ggattaccga aaaagtttac ggatgtggga gtttgttgag agagcactag gagtttcagg
      121 ctccaagttt caagtgtcag gttgaccgcg gacggcagac cttttttcag acacggtcag
      181 cagtcttccg tctgtggtcg tttctttctt ctttattatg aaacgttcat ttcgtcatgt
      241 gggatacatc gtccgcgact tgaagcaatc ggtcgcggat tttcaacgcc tgttcgactt
      301 gcaagatgaa gacatccgca tcatcccgcc ctttgagctg cctgctgaca ctcgatttgc
      361 ctttttccac gctgacggtt tgaccttcga actgatcgag cccatctcgg agcatttcaa
      421 aaatattctc tttaactctg gtgttggcat caatcacatt tgcttcactg tagatgatat
      481 cgaagccgct gtaaatgaca tggcagcgaa aggcatccgt ccagggcatg tcacgccaaa
      541 cggcatcatc accatgccgc atcagaagat ggtgtacttc gatccaaaag atacgggtgg
      601 agtgttgata gaatatattg aagaattacc aatgaatagg aaatagtgcg atggaacaaa
      661 aatcaattaa tatactcggc aactcagtaa cttactggca aggcggagat ggcgcgccgc
      721 ttttcctcat ccacggtggg atcgggaatg cggaggtgca ttggtcgccc gtgtggaatc
      781 aacttacgga gaggtatatg gtcatagcac cgaatctgcc atccttcggt gagacaagcc
      841 ccctggagcg ggcggggttt gctcagatcg cggattgggt gattactctg atggatgcgt
      901 tgaacgtcga gtcggcgaat gtgattggta attcattcgg aggtggagtc agccgtttga
      961 tggcgagcag gcacgagcgg agagtgaagc gcttgatcct cgtcaatggc ggcgcgattc
     1021 cgaacatccc gccgttcgtg gggcgtatga tgcagtcatc cattttcaat ggcatgttca
     1081 acatgatccg caagcagacg ttttcattca aaggtcttgc gcccatgttc gcggataaat
     1141 cgtttttgac caacgagttc atcgcccaag ccgaagcgga ttcgctgggc ttcgtcacac
     1201 tcatgcgtca gtcggcttcg gagggtgttg gcacggaggt ggttccgcaa gttccaacgt
     1261 tggtgttatg gggcgaagac gataaattta caaaaatttc aatggggcaa aatctggcga
     1321 agatgattcc gcatgcggag ttcaagttgt ttacaggcac gggtcacatg ccgcagatcg
     1381 aaaagccaga tgaatttgtg gagaaggtgg ttgagtttct cggttaaaaa aaccgccgac
     1441 gggagtttat cgtcggcggt tctcgtcagt ggtcggttat tttttcataa acttcacaaa
     1501 ttgccatttc gccatggctt gttgaagctc attttcagcc gcattcgcag aaactttgaa
     1561 aagccgctcg aaggt
//
LOCUS       DAOQLC010000368         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_61195_length_1574_cov_19.246873, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000368 DAOQLC010000000
VERSION     DAOQLC010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61195_length_1574_cov_19.246873"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(387..1235)
                     /locus_tag="PL000_22740"
     CDS             complement(387..1235)
                     /locus_tag="PL000_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91756.1"
                     /translation="MERYSKADFPIASSVWEGYSEGRKRGAAQRMLRAVGLRQKIVEE
                     LEPIHLEIPLFRLQPPRTENSKVIYQERQIKERGWDISVKVPGFATGADLSMYLEGKG
                     LVETSHKECKQIAFPVTIRRYRVDLFLGNLCIARNKLIAEAGNQKTGQIFSRTIIACK
                     KFIPPSPQSEMIVEYDLSKDECGNSKFFLGWGEKMRRNAEVTIPIPGIPSGIQVSISI
                     QSDIQLEFDLEPKIDYKLYPTPEGIGISWEVSRGKAKRKNIRNDKGISQFKQRPKKLI
                     YVKRHV"
BASE COUNT          400 a          436 c          316 g          422 t
ORIGIN      
        1 aagcgggcgc cgatgaatac atcaccaagc ccttccgttt gacggagatc cacgatctca
       61 ttctgaaaat ggttgaggaa agaaaatcat taactggcgg gtaaaccgat tctcgacgcc
      121 tgattcaaaa caaactcacc cacgaaaggt tagcttttcg gggtgagttt gttttacaag
      181 ccaaacaaaa tgcagacgat cgctcgccgg ggaaaaaccc gtggtctcta catgcttttc
      241 gatttgcatt ccatgttagt ctgaaagttt aaccacggag acacagagga aacaattaca
      301 gtgtttcagt gtcttcgtgg ttcaaaacgg cctttcaaag acattcctta caacttatgt
      361 aaaaggtcaa ataaagaatc gcggcattac acatgtctct tcacatagat aagtttcttc
      421 ggtctctgct taaattgtga aatgcctttg tcgttcctga tattttttct tttcgctttt
      481 cctctgctca cctcccagct aataccgatg ccttccgggg tggggtaaag tttataatca
      541 atcttgggct caaggtcaaa ctcaagctga atatcacttt gaattgaaat tgagacctgt
      601 atccccgaag gaattcccgg aatcgggatc gtaacttccg cattgcgccg catcttttcc
      661 ccccagccga ggaagaattt tgaattaccg cattcatcct tcgaaagatc atattcaacg
      721 atcatttccg actggggcga aggcgggatg aattttttac aggcgataat cgtacggctg
      781 aaaatctgac ccgttttctg attccccgct tctgcgatca gtttattcct tgcaatgcag
      841 agattgccta aaaacagatc aacccgataa cgcctgatgg tcaccgggaa ggcaatttgc
      901 ttgcattcct tgtgagaggt ttccaccaac cctttccctt ccaggtacat ggataaatct
      961 gcaccggtgg caaacccagg caccttgacc gaaatatccc agccacgttc tttgatctgc
     1021 ctttcctgat aaatgacttt gctattttcc gtgcggggcg gctgaagacg gaacaacgga
     1081 atttcaagat ggatgggctc caactcttca acgatcttct gacgcagccc cacagcccgc
     1141 agcattctct gcgctgcacc ccgtttgcgg ccttcactat atccctccca cacagatgat
     1201 gcaatcggaa agtcagcctt ggagtaacgc tccaaaatgg ccttcctcca tctcgcaccc
     1261 aatctcaatt gccccaaact ctgttcgatc ttttccttat ttattgcccc cacggctgcc
     1321 cagttctcac gcttccgttc tcgaataaca cccctaaaat gatcaccccg cccgcccttt
     1381 ggaatgggtt ttccgggacc aagatacggc ggaccaaatc cgcacgaaca accggccccg
     1441 tgattgtgac cattacacat ggctcctcct ttcctacata ttctttatag catgaggagc
     1501 atgtgaactt caaggcccta caacgccttc tccaaaatct cgcgcaactc cccctcattc
     1561 agcggaatcg gatt
//
LOCUS       DAOQLC010000369         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_62146_length_1562_cov_34.641672, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000369 DAOQLC010000000
VERSION     DAOQLC010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62146_length_1562_cov_34.641672"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PL000_22745"
     CDS             <1..191
                     /locus_tag="PL000_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91757.1"
                     /translation="VGVETVWEEDKLEARKMLENSYSPHPSSARFVGRTAYYRDCVFI
                     IVLKCRVVITCFEKVERK"
     gene            complement(283..1485)
                     /locus_tag="PL000_22750"
     CDS             complement(283..1485)
                     /locus_tag="PL000_22750"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91758.1"
                     /translation="MADIFRKYIGEYKKTHRLSYEQSKAVSAIINCRTPAMGGVMKTC
                     SVCKALEFHYKACKNRHCPLCGAFEKAQWLEAQKKWVLPIHYFQVVFTIDHVFNTLAA
                     FNQKLIFDLFIKVAAQLLKEFGQKYLGGEIGFSMVMHTWGQTMQEHLHLHFIVTGGAL
                     ISAPTGYSWNAASHKFLFPVKLLSNQFRNRFCFEISRLWVDGKLNTNDGALDVQAMLT
                     EGMSKNWEVYIQPPLYDKEKLIEYLGRYIFRIAISNHRIVAVGKDTVTFKYFDNRDDG
                     KEKLMTLPVMEFIRRFLSHVLPDGFVRVRHFGLHHASCRAKLQHARRVLGLPFQLPAT
                     LKLKFLDWFKQITKSDEDPRICKFCGQGLMLPIREFGPVFGWRLKAHALLGLLTSWKN
                     VLPNFVLG"
BASE COUNT          468 a          341 c          383 g          370 t
ORIGIN      
        1 gggtgggcgt ggaaactgtt tgggaggagg acaaacttga agccagaaaa atgcttgaaa
       61 attcgtactc tccccacccg tccagtgcac gctttgttgg acgaactgct tattataggg
      121 attgcgtttt tattatagtc ttaaaatgta gagtggtaat cacctgtttc gagaaagttg
      181 aaagaaaata aaagaggtgc gcgatcatgt tagggcaaaa ggagataaag cgagacataa
      241 aaacatcaag caggcagagt ccctcgcccg cagacgcgca aatcaaccca gaacaaagtt
      301 gggcaaaaca tttttccatg aagtgagtaa accaagaagg gcatgagctt tgagacgcca
      361 gccaaagacg ggaccaaact cgcggatggg caacatcaag ccttgaccgc agaacttgca
      421 aatgcgtggg tcttcatcag acttggtaat ctgtttgaac caatcgagaa acttgagttt
      481 aagggtggcg ggcaattgaa aaggcagacc caaaacgcgg cgagcgtgtt gtagtttggc
      541 gcggcaggaa gcatggtgta aaccaaaatg acggacacgc acaaagccat caggcagaac
      601 atgagagaga aaacggcgaa taaactccat cacgggcagc gtcatcaact tctctttgcc
      661 atcatcgcga ttatcgaaat acttgaaagt gaccgtatct ttgccaacag ccacaatgcg
      721 atgattggaa atggcaatgc ggaagatata gcgaccaaga tactcaatca acttctcttt
      781 gtcatagagc ggaggctgaa tatagacttc ccaatttttg gacatacctt cggtcagcat
      841 agcctgaaca tccaacgcgc cgtcatttgt attgagtttg ccatccaccc agagtcggga
      901 gatttcaaaa caaaagcgat tgcggaattg attggaaagt agtttgacgg gaaagaggaa
      961 cttgtgcgaa gccgcattcc aagagtagcc agtgggagcg gaaattaacg cgccgcccgt
     1021 aacaatgaaa tgaagatgga gatgctcttg catagtttgt ccccaagtgt gcatgaccat
     1081 cgaaaagccg atttcaccgc cgagatattt ctgtccaaac tctttgagca gttgagccgc
     1141 aactttgata aataaatcga aaatgagttt ctgattgaac gctgccagcg tattgaaaac
     1201 atggtcaatg gtgaagacga cttggaaata atgaattggc aaaacccatt tcttctgcgc
     1261 ttccaaccat tgcgccttct caaatgcgcc gcataaaggg cagtggcgat tcttgcaggc
     1321 tttgtagtga aattccaaag ccttgcacac ggaacaggtt ttcatcacac cacccatcgc
     1381 gggcgtgcgg caattgataa tagcgctcac ggctttcgat tgctcatacg aaagccgatg
     1441 agttttttta tattcaccaa tatatttgcg gaagatgtca gccacatcgt atttcacccc
     1501 tggctgttgc gtcgatgtat tttctatata tcacctatag ttgtgcgagg ttcgtccaac
     1561 gg
//
LOCUS       DAOQLC010000370         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_63641_length_1543_cov_25.313844, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000370 DAOQLC010000000
VERSION     DAOQLC010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63641_length_1543_cov_25.313844"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="PL000_22755"
     CDS             <1..1402
                     /locus_tag="PL000_22755"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1939 domain-containing protein"
                     /protein_id="HNC91759.1"
                     /translation="TPLKYLLEIRFRVRIKDKGAIRMGVMMQTFYWDCPREDKKEFQW
                     WNYINREIPNLAKAGFTSLWLPPVHKAANLGGPSMGYDPYDFYDLGEFDQKGSVPTWF
                     GTRSELSQLIESAHRHGLSVIADMVLNHNSGADLQEVNPITGQSRWTVFNPMSERFPR
                     NWESFHPSMYESWDEMTFGDMPDLSHRNPYVYAELLKLARWLIEEVGFDGFRYDFVKG
                     YGANTITAIQEYRYMKNGAYHRPYGVAEYWDNASNTLNWVNAANFSNSNPVDAFDFPL
                     RELLKGLCDSYGFSLKSLVNGESILRAQPQSTVTFVENHDLRDEGRPITNDKLLAYSY
                     IFTHEGYPCVFWKDYFNYNLALRDTPNGIDALISAHEKYAGGNVSTLYLSDDLYIMQR
                     GGYENQPGLIYVLNNHGERWQGEWVNTQWTNASFQPVAWWSKNDLSRPVNQSTGADGR
                     GQFFAPPRGYAVYALK"
BASE COUNT          388 a          397 c          393 g          365 t
ORIGIN      
        1 aaccccatta aaatacctgc ttgaaatccg ctttcgtgtg cggataaaag ataaaggagc
       61 aatacgaatg ggtgtgatga tgcaaacctt ctactgggat tgtcccagag aggataaaaa
      121 agaattccag tggtggaact atatcaaccg tgagattccc aaccttgcga aggcgggttt
      181 tacttcgttg tggcttccgc ctgtccacaa ggcggccaat ttgggtggac cttcgatggg
      241 atacgaccca tacgattttt acgacctcgg cgaattcgac cagaagggga gcgtccctac
      301 ctggtttgga acgcgttcgg agctgagtca actcattgaa agcgcacaca ggcacgggtt
      361 aagtgtgatc gcggatatgg tcctcaatca caacagtgga gcggatttgc aggaagtgaa
      421 tcccatcacg gggcaatcgc gttggacagt gtttaatccc atgagcgaac gtttcccgcg
      481 caattgggaa tcgtttcacc ccagcatgta cgaaagctgg gatgaaatga ccttcggcga
      541 tatgcccgac ctttcgcatc gcaacccgta tgtgtatgcc gaattgctca agctcgcacg
      601 ctggcttatc gaagaggtcg gctttgatgg attccgctat gactttgtca aaggctatgg
      661 cgcaaacacc atcacagcca ttcaggaata ccgttatatg aagaacggcg cgtatcacag
      721 gccgtacggc gtggcggaat attgggacaa tgcctcgaac acgctcaact gggtcaatgc
      781 ggcgaacttc tccaactcga accctgtgga tgcgtttgat tttccgctgc gcgaattgct
      841 caaagggctg tgcgattcgt atggattcag tttgaagagc ctcgtcaacg gtgaatccat
      901 tttgagagcc cagccgcaaa gcacggtgac ctttgttgaa aaccacgatc tgcgcgacga
      961 aggccgaccc atcaccaacg ataaactgct ggcttattcc tacatcttca cgcacgaagg
     1021 ttatccctgc gtattttgga aggactattt caattacaac cttgccttgc gtgatacgcc
     1081 caatggcatc gatgcgctca tcagcgcgca cgaaaaatac gcaggcggaa atgtcagcac
     1141 gctgtatctt tcagatgatc tctacatcat gcagcgcggc gggtatgaaa atcagcctgg
     1201 attgatctat gtgctcaata atcacggtga acgctggcag ggagaatggg tcaatacgca
     1261 atggacgaac gcctcttttc aacccgtggc ctggtggagc aaaaatgacc tgtcccgccc
     1321 cgtaaaccaa tccactggcg ccgatggccg cggacaattc ttcgctcccc cgcgtggata
     1381 cgctgtgtac gccctgaaat aacatccgcg tcatgcgaga attcgtgtta gattctccct
     1441 gccttgttac ggtcatggcc ccgtacctcc gcagcaacgg gctttgattt ttataattga
     1501 cctcttgcat aagtaaagtg ttgtagacca caaagtcacc aag
//
LOCUS       DAOQLC010000371         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_63788_length_1541_cov_13.298116, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000371 DAOQLC010000000
VERSION     DAOQLC010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63788_length_1541_cov_13.298116"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PL000_22760"
     rRNA            complement(<1..554)
                     /locus_tag="PL000_22760"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(695..771)
                     /locus_tag="PL000_22765"
     tRNA            complement(695..771)
                     /locus_tag="PL000_22765"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Ile,seq:gat)
     gene            complement(813..888)
                     /locus_tag="PL000_22770"
     tRNA            complement(813..888)
                     /locus_tag="PL000_22770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(853..855),aa:Ala,seq:tgc)
     gene            complement(1035..>1541)
                     /locus_tag="PL000_22775"
     rRNA            complement(1035..>1541)
                     /locus_tag="PL000_22775"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          331 a          449 c          362 g          399 t
ORIGIN      
        1 tttcagttac tatttcactc ccctaacagg ggtgcttttc acctttccct cgcggtacta
       61 gttcactgtc ggtcttcatc gagtatttag ccttatgccg tggtcggcac gaattcacac
      121 cgaatttcac gtgtacggtg ctactcaggg taccactagg tttgcttaga ttttaaacta
      181 cgggactgtc accctctatg gtctcacttt ccagagtgtt cgtctaatcc tcgcattcca
      241 cattgtggcc ctataacccc gccaggataa atcccaacgg tttgggctct tccgcgttcg
      301 ctcgccacta ctaacggaat cgattctcgt tcttttcctg cggatactga gatgtttcac
      361 ttctccgcgt attgcgcgac tgatcgtatg aattcggacc agtgcgacac cgaatgaccg
      421 gtgccgggtt tccccattcg gaaatcttcg gatcaaagcg tgcgtgccgc tccccgaagc
      481 ttatcgcagc ttgctgcgtc cttcgtcgcc tgatgaagcc aaggcatcca tcaattgctg
      541 taactggttt atgcaaacgc tcaatattct aggcgtttat cttacccact tacatttcta
      601 tctaccctag actttcaaag aacaaaatcg gttgattttg aaacatcgtt ctcttccacc
      661 cgctgccaac cccaccgtag ccttctagct caaatggtgg gcccagatgg acttgaacca
      721 tcgaccccgc gcttatcaag cgcgtgctct aaccaactga gctatgagcc cgagattgga
      781 aaatggcgga aaccagcctg gggcggtagg cttggtggag ccggtcggat tcgaaccggc
      841 gacaccctgc ttgcaaagca ggtgctctac caactgagct acggccccat gcaggtaaat
      901 ggatgttata taaagaacga tactctcaaa atttactttg tgcgatcaat caggacccag
      961 aattagcagg ttgccctgcc tcttctttcg accatcggtg cgacagtcgt tagactgtgt
     1021 tgccgtctcc ttagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     1081 gacttcgtcc caatcaccag cctcacctta gggcgccgcc tccattgctg gttggcaaac
     1141 gcacttcggg cgaaaccggc tttcatgacg tgacgggcgg tgtgtacaag gcccgggaac
     1201 gtattcacgg cgccgtagct gatgcgccat tactagcgat tccaacttca tgtagtcgag
     1261 ttgcagacta caatctgaac tgggctcggt tttgaggatt tgctccacct cgcggtattg
     1321 cgtccctctg tgccgagcat tgtagtacgt gtgcagccct agccgtaagg gccatcctga
     1381 cttgacgtca tccccacctt cccaccctca aagagggttt gtctccctag agtgcccaac
     1441 ttaatgatgg caactaagga caggggttgc gctcgttgcg cgacttaacg caacatctca
     1501 cgacacgagc tgacgacagc catgcagcac ctgtgcacca g
//
LOCUS       DAOQLC010000372         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_64121_length_1537_cov_15.989879, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000372 DAOQLC010000000
VERSION     DAOQLC010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64121_length_1537_cov_15.989879"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..695
                     /locus_tag="PL000_22780"
     CDS             84..695
                     /locus_tag="PL000_22780"
                     /inference="COORDINATES: protein motif:HMM:NF017666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HNC91760.1"
                     /translation="MSSVAEIANINRMGYVGKKPGSGSADRDSDSWYTPAGYINAARA
                     VMGGIDLDPFSSDRANENVKAARILTEDRSAFSNAWRKDKRKYPEGVCVWMNPPYSGG
                     IIGKAVDTFIENLTAGNIRQAVVLVNNATDTQWFKTLRENSSAVCFTHHRISFESPDG
                     KRVSGNTRGQTFFYFGPESRAEAFVKEFSRFGWCISKSHGWRS"
     gene            692..1153
                     /locus_tag="PL000_22785"
     CDS             692..1153
                     /locus_tag="PL000_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91761.1"
                     /translation="MNAVVQSQGRQANRNGRVFEDLIAQRLRSEGYVSLTKLPDKFEF
                     PFFVAQLRGRFSSIYGSPLRVDFFVWHPEKYPNGMIIECKYQETNGSADEKFPYTVAN
                     LRKANIPAVLLIMGRGPKPCSVEWCLKQQDEHLKVFKDLESFMKSTNRDLF"
     gene            1153..>1537
                     /locus_tag="PL000_22790"
     CDS             1153..>1537
                     /locus_tag="PL000_22790"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HNC91762.1"
                     /translation="MSAQLVAVSSIKVENRKRPVDSATVAELKKSIEQQGLLQNIGVQ
                     ESANGYRLVFGAHRLEAIKSLGWPEVSALVFPETASEDECLLAELQENNARKELTGAE
                     RKAFAAEVGRIIAKMQENCETDSNCD"
BASE COUNT          377 a          387 c          446 g          327 t
ORIGIN      
        1 ggcaacaaaa aacccgccga agcgggctta gtgcgccggt gacagccggc taattcaaaa
       61 cctggcaaag aggtcatgaa ctgatgagta gtgtagcaga aattgcgaac atcaaccgca
      121 tggggtacgt gggcaagaag cccggaagcg ggtcggcgga ccgtgattcc gactcctggt
      181 acacgcctgc gggttacatc aatgccgcgc gcgcggtcat gggcgggata gaccttgacc
      241 cgttttcatc ggaccgcgca aacgaaaacg tcaaggccgc gcgcatcctg accgaggacc
      301 gctcggcttt cagtaacgca tggcgcaaag acaagcgcaa atatcctgaa ggcgtttgcg
      361 tctggatgaa ccctccatat tccggcggca tcatcggcaa ggccgtggat acgtttattg
      421 aaaacctgac tgccgggaat attcgacaag ccgttgttct ggtgaacaac gcaaccgata
      481 cgcagtggtt taaaacacta cgggaaaaca gttcagcggt atgtttcacg catcatcgga
      541 tctcgtttga atcccccgac ggcaagaggg tcagcggaaa cacgcgcgga cagacgttct
      601 tttatttcgg cccagagtcc agggcggaag cgtttgtgaa agagttctcg cgctttgggt
      661 ggtgtatcag caagtctcac gggtggcgct cttgaacgcg gttgtgcagt ctcaggggcg
      721 tcaggccaac cggaatggcc gcgtttttga ggacttgatt gcacagcgtc tgcgtagcga
      781 agggtatgtg tcgctgacaa agctgccgga taaattcgag ttcccgtttt tcgtggcaca
      841 gctccggggt cgattctcca gcatttatgg ttccccgcta cgggtcgatt tcttcgtttg
      901 gcatcccgaa aaatacccta acgggatgat tatcgaatgc aagtatcagg aaaccaacgg
      961 gtctgccgac gaaaaattcc cctacaccgt ggccaatctc agaaaggcca atatccccgc
     1021 tgttttgttg attatgggcc gcggaccaaa gccgtgctcg gtcgaatggt gcctgaaaca
     1081 gcaggacgaa catcttaagg tttttaaaga cctggaatcc ttcatgaaaa gcaccaatcg
     1141 ggatctgttt tgatgagcgc gcaactcgta gccgtatcca gcattaaggt tgaaaaccgc
     1201 aagcgcccgg tcgatagcgc gaccgtcgcg gaactgaaaa agtcgattga acagcaggga
     1261 ttgctgcaaa acattggcgt tcaggaatcc gctaacgggt atcggctggt cttcggtgcg
     1321 catcgccttg aggcgatcaa gtctctcggg tggcctgaag tttcggcgct tgtctttcca
     1381 gagaccgcca gcgaagacga gtgcctgtta gctgaacttc aggagaacaa cgcccgcaag
     1441 gaattgaccg gggctgagcg gaaagcgttt gccgctgaag tcgggcggat tattgcgaaa
     1501 atgcaagaaa attgcgaaac ggacagtaat tgcgatt
//
LOCUS       DAOQLC010000373         1524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_65226_length_1524_cov_22.563649, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000373 DAOQLC010000000
VERSION     DAOQLC010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65226_length_1524_cov_22.563649"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PL000_22795"
     CDS             <1..901
                     /locus_tag="PL000_22795"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC91763.1"
                     /translation="EETMKPNPYLQKMGYSNTDRLVIIHTDDIGMCHASVQAFKDLWA
                     VGTITSGAVMVPCPWFPATAQMCRENPEMDMGVHATLNAEWTNYRWGPVSEAGAGSGL
                     RDEAGYFHQWHQAVYDHASPEEVEAEVNAQIEMALKAGIDVTHVDSHMGTIMNPKYIQ
                     SYVQAASSRLLPNMLPRLDAKGIEMMGVSDEDKVSYAPIMEMLEGIGVPTMNGIVAMP
                     LDQPDGQMEIAKNLLGNLPEGITHFILHPSIDTPELRAIAPDWESRVSNYNTFMSDEL
                     KKFIESEDIKPIGYRAIREAMRK"
     gene            929..>1524
                     /locus_tag="PL000_22800"
     CDS             929..>1524
                     /locus_tag="PL000_22800"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC91764.1"
                     /translation="MTAVTKSQTSIKTRMMYSLMSLGVATPGEAVAGIIAFYIVDVKN
                     LPATWYASFWFFYTLYNAINNPVLGYMSDRTRSRWGRRLPYILFTGLPYAVTFALLFT
                     APFDGKDNPVGLLAYFGTCIVIWEGIYTALATGYYGLLPEMFGDYAERTDVAAKMNIF
                     QTVALVLGAALPPMLANLLGWSGMAAVLAVISVVAIYVG"
BASE COUNT          363 a          367 c          450 g          344 t
ORIGIN      
        1 agaggaaacc atgaaaccaa atccgtactt gcagaaaatg ggatacagca acacagacag
       61 actcgtcatc atccacaccg atgacattgg catgtgccat gcgtcggtgc aggcgttcaa
      121 agatttgtgg gcggttggaa cgattacttc aggagcggtg atggtgcctt gtccgtggtt
      181 ccctgccacc gcgcagatgt gccgcgagaa ccccgaaatg gacatgggag tccatgccac
      241 gctgaacgcg gagtggacaa actatcggtg gggacctgtc tccgaagcag gagcaggctc
      301 gggcttaaga gatgaagcgg ggtacttcca tcaatggcat caggcagtgt acgaccatgc
      361 cagtccagag gaagtggagg cggaagtcaa tgctcaaatt gaaatggcgt tgaaggcggg
      421 cattgatgtg acgcatgttg attcgcacat gggcacgatt atgaatccga agtatatcca
      481 gtcgtatgtg caggcagcat cgagccgttt gctgccaaac atgctgccac gcctggatgc
      541 aaagggaatc gaaatgatgg gcgtgagcga cgaggataag gtttcgtacg cgccaatcat
      601 ggaaatgctc gaagggattg gcgtgccgac gatgaacggc attgtggcga tgccgcttga
      661 ccagccagat gggcaaatgg agattgccaa aaacttgttg ggcaatttgc ccgaaggcat
      721 cactcatttc attttgcatc cgtccattga tacgcccgaa ctgcgagcca ttgcccctga
      781 ctgggaaagc cgcgtttcaa attacaacac gttcatgagc gatgagttga aaaagttcat
      841 cgaaagcgaa gacatcaagc cgattgggta tcgcgcaatt cgcgaggcaa tgcggaagta
      901 atacgggtag aacgccaaag gagttcacat gacagccgtg acaaaatctc aaaccagcat
      961 caagacccgc atgatgtatt cgttgatgag ccttggggtt gccacaccag gtgaagccgt
     1021 ggcgggcatc attgcgttct acatcgtgga tgtgaaaaac ctgcccgcca catggtatgc
     1081 gagtttttgg ttcttttaca ctctgtacaa tgcaatcaac aatcccgtgc ttggatacat
     1141 gtctgaccgc acacgttcgc gctggggaag gcgcctgccg tatattttgt ttacgggtct
     1201 gccctatgct gtgacctttg ccctgttgtt caccgcgccg ttcgatggca aggacaatcc
     1261 cgtcgggctg ctggcttact ttggaacctg catcgtgatt tgggaaggga tttacaccgc
     1321 gctggcgact ggctattacg gcttgctgcc tgaaatgttc ggcgattacg ctgagcggac
     1381 agatgttgcc gcgaagatga acatcttcca aacggtggcg ctggtgcttg gcgcagcgct
     1441 gcccccgatg ctggcaaatt tgctgggatg gtcgggcatg gcggctgtgc tggctgtgat
     1501 ttcggtcgtc gcaatctatg tggg
//
LOCUS       DAOQLC010000374         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_65736_length_1518_cov_13.768284, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000374 DAOQLC010000000
VERSION     DAOQLC010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65736_length_1518_cov_13.768284"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1245
                     /locus_tag="PL000_22805"
     CDS             73..1245
                     /locus_tag="PL000_22805"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HNC91765.1"
                     /translation="MTCPQCENIVERPDLPHTVRRCEGCGRELRIHEPGAHGIGFNIR
                     KGDQVVIPEGWLKLSLNPLKSSGRFSRYGLQWFAEQIHLEELPKKKNDIAAEIDRLET
                     KCDQILEASDLLAGLDLANPDHTDQIIAKLRERQDSIEWWAFLMGTFISFLRDATEKC
                     DVLQAVWAMGCVERCRSMLLFKEHLEEVVWMGQSAKRVVDILHTWDNNKTNADEGFWQ
                     ITFRENVYAISQVFAIPLVFIQEAAYVGGMNIDRQNAKLVDYLFSNESSQEAVLVEIK
                     TPATKLLGPKYRGTYRPSTELSGAVMQALDYRRTLSKNLTSVVEGTSHKLSAFAPKCV
                     VVIGNGALELDNDLKRGAFEMFRSNSKEVEIITYDELFRKLEVLASLFSLTRQKQK"
BASE COUNT          393 a          413 c          372 g          340 t
ORIGIN      
        1 cgctggagca cactcgccgc cttacggcgt caaggtgctc acctccaacg ttagacctct
       61 ccgcgaacca atatgacctg tccccagtgt gaaaacattg ttgagcgtcc tgacctgcca
      121 cacaccgtta ggcggtgcga agggtgcggg cgggaattac gcattcatga acctggcgca
      181 catggcattg gcttcaatat tcgcaagggt gaccaagttg tcatccctga aggctggctg
      241 aagctctcac ttaacccctt gaagagttca ggtcggtttt cccgatacgg tcttcaatgg
      301 ttcgctgagc aaatacatct cgaagaactg cccaagaaaa agaatgatat agccgcagaa
      361 attgaccgac ttgagaccaa atgcgatcaa atcttggaag cgtcagatct gcttgcaggc
      421 cttgatctcg cgaatccaga tcataccgac caaataattg ccaaactccg ggagcgtcag
      481 gactcgatcg aatggtgggc cttccttatg ggaacattca tcagcttcct gcgtgacgcc
      541 acggaaaagt gcgatgtgct gcaagccgtg tgggcaatgg gctgcgtcga gcgctgtagg
      601 tcaatgctgt tgtttaagga gcatcttgaa gaagttgttt ggatgggcca gtcggccaag
      661 agggttgtag acatcctgca cacatgggac aacaacaaga ccaacgccga tgaaggtttc
      721 tggcaaatca cgttccgtga gaatgtctat gcgatctccc aagtcttcgc catacctctc
      781 gtattcattc aagaggctgc atacgttggc ggcatgaaca tcgaccgcca gaacgcgaag
      841 ttagttgatt acttgttctc caatgaatca tcccaagagg cggtcctcgt tgagattaaa
      901 accccagcga ccaaactact tggccccaaa tacagaggca cctatcgtcc gtcgacagag
      961 ctttccggtg cagtcatgca ggcactcgac tacagacgta cgctgtcgaa aaatctcaca
     1021 tctgtagtcg agggcactag ccacaaacta agcgcatttg caccaaagtg cgttgtcgtc
     1081 atcggaaacg gtgcactgga actcgacaat gacctcaaac gcggggcctt cgaaatgttt
     1141 cgttcaaact ccaaagaagt agaaatcatc acctacgacg agttgtttcg aaaactcgag
     1201 gtactggcct ctctattcag ccttacgcga cagaagcaga aatgaggtct aacttatcat
     1261 tccatcggac ccgcgctaaa agccgcgctg gccgggtaaa cgttcacccc cctgctcagt
     1321 gaacgttaat tttcctgaaa tttacgccgc agcgaaaatt tctatgtcat gatgtcggca
     1381 cccaacgacg acccgccccg atgtacctgc gtaagcacga ccttctccag atggacgacg
     1441 actggctgaa aaggctgccg gcgggtaaac gttcaccccc ctgctcagtg aacgttaatt
     1501 ttcctgaaat ttacgccg
//
LOCUS       DAOQLC010000375         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_65831_length_1517_cov_21.989740, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000375 DAOQLC010000000
VERSION     DAOQLC010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65831_length_1517_cov_21.989740"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="PL000_22810"
     CDS             complement(<1..>1517)
                     /locus_tag="PL000_22810"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC91766.1"
                     /translation="AEEALNTSRQLLQAIVDNAPVRIFWKDTELRYLGCNPSFAVDAG
                     EKDPQNIIGKDDYELSWRNQADFYRADDQKVMSTGVGKFFYEEPQSTPDGKLIMISTS
                     KVPLRDENGQTIGVLGIYQDITEQKQAEELILAQRDLAYIISSATSNKDAFKHCLAIA
                     LRISGFDSGGIYLFNEGKQTLDLVHHHGLSEKFVQSSSSYPVDAPNVKTLFSGLSFYF
                     NDADLRDRETFLTEGIHSYASLPIQHHGQVIGCINVASHAMVDVPDFTRRALEVTASE
                     IGNIITHLQTEASLHESEEKYRSLINSQESSIAMLDAEGIYHYVNEIGAARLNGSVQD
                     IIGKKAQDLLPPETVEWKINVLQQVIETGQGIVQEVQLQEASGKFWHRISVQPIRDAN
                     GQVKLAMLTSIDITERKKAEAELQNLKDRLLEAQQLAHVGNWEWDTHKQVLFWSEEVY
                     RIFGVNPAEFLPTVEAFEGAIHPEDKESFLAQRAKMLEEQQSASIDHRIILPDGRV"
BASE COUNT          338 a          375 c          372 g          432 t
ORIGIN      
        1 acccgcccat caggcaggat gatacggtgg tcaatggacg cagattgttg ttcctccagc
       61 atttttgccc gctgtgccag aaaactttcc ttgtcttctg ggtgaatggc tccttcaaat
      121 gcctccactg tgggaaggaa ttcggcaggg ttcaccccga agatgcgata gacctcctcc
      181 gaccagaaca acacttgttt atgagtatcc cattcccagt tgcccacatg agcgagctgc
      241 tgcgcttcga gaagacggtc ttttaagttc tgaagttccg cttcagcctt ttttcgctct
      301 gtgatgtcga tcgaagtcaa catcgccagt ttgacctgtc cgttggcatc tctaatgggt
      361 tgcacgctaa tacgatgcca gaatttcccg ctggcttcct gtaattgcac ttcctgaaca
      421 ataccctgtc ctgtttcgat cacctgctgc aaaacattga tcttccactc cacagtttcg
      481 ggagggagca ggtcttgtgc ttttttaccg ataatgtcct gtacagaacc attgaggcgc
      541 gcggccccga tctcgttgac gtaatgatag atgccttccg catccagcat ggcaatggaa
      601 gattcctggc tgttgatcag gctgcggtat ttttcctcgc tctcatgcag ggaggcttcc
      661 gtttgcaaat gggtgatgat gttgccaatt tctgaagcgg ttacttcaag ggcacggcgg
      721 gtgaaatcag gcacatcgac catggcatgt gaggcgacgt tgatgcagcc gataacctgc
      781 ccatgatgtt gaatagggag gctggcatac gagtgtatcc cctcggtgag aaatgtttcc
      841 ctgtctctta aatcggcatc attgaagtag aacgataaac cactgaacag cgtttttaca
      901 tttggggcat caacaggata tgacgaactc gactgaacga acttctcact caggccgtga
      961 tggtggacga gatcgagagt ttgttttcct tcattgaaca gataaatgcc gccactgtca
     1021 aaccccgaaa tcctcagggc aatggcaagg cagtgtttaa aggcatcttt atttgatgtg
     1081 gctgaactga tgatatatgc aagatctcgt tgcgcgagaa tcaattcctc cgcctgtttt
     1141 tgttcggtga tatcttgata aattcccaat acgccaatgg tctgcccatt ttcgtcccgt
     1201 agcggtactt tggaggtgct gatcatgatg agttttccat cgggggtgga ttgcggctcc
     1261 tcgtagaaga atttcccaac ccctgtactc atcacctttt gatcgtcagc gcggtaaaaa
     1321 tcggcctgat ttctccaact taattcataa tcgtctttgc caatgatgtt ttgcgggtct
     1381 ttttcgccgg cgtccactgc aaaactggga ttgcagccaa gatagcgcag ttctgtgtct
     1441 ttccagaaaa ttctaacagg ggcgttatct acaatggcct gtagcagttg tcgggatgtg
     1501 ttgagcgctt cttccgc
//
LOCUS       DAOQLC010000376         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_66272_length_1512_cov_11.859986, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000376 DAOQLC010000000
VERSION     DAOQLC010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66272_length_1512_cov_11.859986"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1512
                     /locus_tag="PL000_22815"
     CDS             <1..>1512
                     /locus_tag="PL000_22815"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC91767.1"
                     /translation="DQRAQWMTEYMMQYLAGTSGLTYVAPDLASRTFSNFVIILFLHV
                     FLPLIGFLGIYIHSLRLSRERWWSPRWVSIQAVVGLVILSLIKPAQSAMPADLSHLIP
                     SVPMDALYLGFLPLIDSFGNLIFWGLAILVGGSLFLLPWLASGRNLGPASVTDPKCTG
                     CNICYAECPFDAIRMNDRSDSSGYHKLAIINAAQCTGCGICVGACPTDAIDLKGGYNG
                     EQVFGAVKGALSQEKKNGSPVTVLFASQRDEALDGLPAELNVSKENAPVAVSTIGEKK
                     AARVITAVLPSVSAVNIEWIKSLHNEGARDVVLLAPPYDDGVFREDAHWIANRLRARP
                     ALVTKDLHWLETTPGEAKSVLNFLNDLHRAETQAKKSVPVLPVLKERSKLMPSLVSAL
                     VGTIFLFAIFALALPLDVPTGMSAAQGSAIRVALDLKGKISAANIPEGMTLPEGADAE
                     KIFGGTHYPVSIRLVVDGKTVLEETYQPSGVSGNGRISALEFVQIPAGSHQIE"
BASE COUNT          314 a          396 c          424 g          378 t
ORIGIN      
        1 gggatcaacg cgcgcaatgg atgaccgaat acatgatgca atatctggcg ggcacatcgg
       61 gtctcaccta cgttgcgccc gacctcgcct cgcgcacatt ctccaacttc gtcatcattc
      121 tttttctgca cgtctttctg ccgctcatcg gctttctcgg catttatatt cacagcctca
      181 ggctctcgcg tgagcgttgg tggtcgccgc gctgggtcag cattcaagcc gtggttgggt
      241 tggtgattct ctcgctcatc aaacccgctc aatcggcaat gcccgcagac ctatcgcacc
      301 tgattccatc cgtgccaatg gacgcgctgt atttgggctt tttacctttg attgattcct
      361 tcggcaattt gattttctgg ggattggcga tccttgttgg cgggagtttg ttcctgctcc
      421 cctggctcgc ttcgggacgt aatctgggac ctgcttctgt taccgatcca aaatgtactg
      481 gctgtaacat ttgctacgcc gaatgtccat tcgacgctat ccgcatgaat gaccgcagtg
      541 attcttcggg ctaccataaa ctggcaatta tcaatgccgc gcaatgcaca ggctgtggaa
      601 tctgtgtggg agcctgccca acagatgcaa ttgacttgaa aggcggctac aacggggaac
      661 aggtctttgg cgcagtgaag ggtgctctgt ctcaggaaaa gaaaaatggc agccctgtca
      721 cggtcttgtt tgctagtcaa cgagatgaag cgctggacgg cttgcccgca gagttaaacg
      781 tcagcaaaga gaatgctcct gttgctgtat caactatcgg tgagaagaaa gcggcacgcg
      841 tcatcacggc ggtgctgcca agtgtgagcg cggtcaacat cgagtggatt aaatccctgc
      901 acaatgaagg cgcgcgcgat gtcgtcctgc tggcgccgcc atatgacgat ggcgtgttcc
      961 gcgaagacgc gcattggatc gcgaaccgcc tgcgcgcgcg acccgcgctg gtgacgaagg
     1021 atttgcactg gctggagaca acgcctggcg aagcgaaatc ggttttgaat tttctcaatg
     1081 acttgcaccg cgctgaaacg caggcgaaga agtctgtgcc tgtgttgcct gttttgaagg
     1141 agcgcagcaa gttgatgcct tcgttggtaa gcgcgttggt gggcacgata tttttgtttg
     1201 cgatttttgc ccttgcgctt ccgttggatg ttcctacggg catgtctgcg gcgcaaggct
     1261 ctgccattcg cgttgcgctg gatttgaaag gcaagatttc agcagccaac attcccgaag
     1321 gtatgaccct gcccgaaggc gcggatgcag aaaagatttt cggcggcacg cactaccccg
     1381 tcagcattcg gctggttgta gatggcaaga ctgtattgga agaaacctat caaccttcag
     1441 gagtcagcgg caatggacgc atttctgcgc tggagtttgt acaaattcca gcgggctcac
     1501 atcaaatcga ga
//
LOCUS       DAOQLC010000377         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_68831_length_1484_cov_12.608118, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000377 DAOQLC010000000
VERSION     DAOQLC010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68831_length_1484_cov_12.608118"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1325)
                     /locus_tag="PL000_22820"
     CDS             complement(93..1325)
                     /locus_tag="PL000_22820"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC91768.1"
                     /translation="MHITILALGSRGDVQPYAALGKGFKADGHQVRFITFEDFAPLIT
                     ESELDFHSIQGNAKELVANAGADLPALVRSFGSLAEGYARDLSAPYLGETDLIINQLP
                     AGLFGFDLAEKYDVPMVQAAVIPLARTRGFPLMGLPKLPLPGYNKATYYLGEQIAWQM
                     FRTVINRWRKKTLNLPPLPLLGYFDQLGTRRFPIVNGFSQYVVARPADWNEYVHITGY
                     WFPEDRNWEPPHDLSAFIEAGSPPVFIGFGSMPVKDPQRITNIILDSLKQSGQRGILH
                     MGWGGLGNQSLPDHVFKINYAPYEWLFPRMAMSIHHGGSGTTAFGLRAGVPSCVVSFV
                     FDQHYWGERIAQLGAGPRPIRFKELTTERLREAIQLGVGDPEMRQKATELGRKIQTEN
                     GIKNALKVFEKITYRDEN"
BASE COUNT          400 a          376 c          347 g          361 t
ORIGIN      
        1 aggccagtgc ccatacgcca aacagcgcag aaaggaggag cagaagccaa ttctttttca
       61 ggtaaacgcg gatatggctg gtcatcacag ttttaattct cgtcacgata ggtaattttc
      121 tcgaacactt tcagagcgtt tttaattcca ttttctgttt ggatcttgcg cccaagttcg
      181 gtggcttttt ggcgcatctc aggatcgcca acaccgagtt gaatagcttc ccgcagtcgt
      241 tcggtagtca attccttaaa tcgaataggc ctgggacccg cccccaactg ggcgatgcgt
      301 tccccccaat agtgctggtc aaacacaaac gacacaacac aggatggcac accagcgcga
      361 agaccaaagg cagttgttcc tgagccacca tgatgaatcg acattgccat tcgtgggaac
      421 aaccactcat acggggcata attgatcttg aaaacatgat caggtaacga ctgatttcca
      481 aggccgcccc agcccatatg taaaatgccg cgctgcccgc tctgttttaa ggaatcaaga
      541 atgatgttgg tgatgcgctg cggatctttt accggcatac ttccgaaacc aataaacaca
      601 ggcggactgc ctgcttcaat gaatgctgat aaatcatgcg gtggctccca atttctatct
      661 tctggaaacc aataacccgt gatatggaca tattcgttcc aatcggcggg acgcgcgacc
      721 acatattgac taaacccgtt cacgattgga aaccgcctcg tacccaattg atcaaagtaa
      781 cccagcagcg gcaacggagg caggttgagc gtttttttac gccaacgatt gattacagta
      841 cgaaacattt gccacgctat ctgttcgccc aaataataag ttgctttgtt atagcccggc
      901 aggggaagtt ttggaagacc cataagagga aatccccgtg tccgagccaa tggaattacg
      961 gctgcttgta ccatgggcac atcgtatttt tccgccaagt caaaaccgaa taatcctgct
     1021 ggaagctgat taataatgag gtctgtttca cccaggtatg gtgcagacaa atcacgagcg
     1081 taaccttctg ccagagatcc aaaggaacgt acaagggcag gcaggtctgc tcctgcattg
     1141 gcgaccaatt ccttcgcatt tccttgaatt gagtgaaagt ccagctcgct ttcggttata
     1201 agtggtgcaa agtcttcaaa ggtaataaaa cggacttgat ggccgtcagc tttgaaccct
     1261 ttgccgaggg cagcataggg ctgaacatca ccgcgtgaac ctagtgcaag aatcgttatg
     1321 tgcatggaaa atatcttgag tgaagcacct gactatttat tcagtaccca atcccaactc
     1381 tggaataggc ggaagtgagc cgctgtacaa ccaattgttg aagaactcgc ccaattcctg
     1441 tccgctgatc tcctcggcca cagcgacaaa atcttcggtg gtgg
//
LOCUS       DAOQLC010000378         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_69101_length_1481_cov_21.809257, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000378 DAOQLC010000000
VERSION     DAOQLC010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69101_length_1481_cov_21.809257"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PL000_22825"
     CDS             complement(<1..874)
                     /locus_tag="PL000_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91769.1"
                     /translation="MSKKLFTVLLLAVFVLAQFSMASAATVAGADITLTRDSEFLSAA
                     AAAPWATATFDWTLGALPAGTTYYVDIYAREDSAAYTNANCQNVATVNGGVAANGTFT
                     YDFTGVTTANTEVYEFVMVVDDAGSCANDVAVEAADVVMHSAYVDGSAIDGFGAFPHD
                     DFTVAGDFVACNAFEIWALGADNYATGTNPYSGFGSWNPKTTGTFATPAPVGADTSLL
                     SWNFTFPATASGPWEMSINPEDAAGNMYDPTLAAPYTFYSTTPIASELEDCADFSDTA
                     GHADEVYIRYLADLG"
     gene            complement(1146..>1481)
                     /locus_tag="PL000_22830"
     CDS             complement(1146..>1481)
                     /locus_tag="PL000_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91770.1"
                     /translation="LFDPNNLLTRGQIVTVMNNIVDNGIANIVPGTHLFSSLMPLAFN
                     DAVKPYRAAAWSDVSLGAFYAEPVVQAYGWGIADGTSATTFSPDQPVTRGEFAKMLYR
                     ALSYYYGSF"
BASE COUNT          354 a          366 c          464 g          297 t
ORIGIN      
        1 ggccgaggtc agccaggtag cggatgtaca cttcatctgc atggccagcg gtgtcgctga
       61 aatcagcgca gtcttccaat tcggaagcaa tgggcgtcgt gctatagaat gtgtaggggg
      121 cagccagggt cgggtcgtac atattgccag ctgcatcttc agggttgatg ctcatttccc
      181 agggaccact tgcagtggcg gggaaggtga agttccagga gagcaggctg gtatccgcac
      241 ctacaggggc cggggtggca aaggtgccgg tggtcttggg gttccaggaa ccaaagccgg
      301 agtacgggtt ggtgccggtg gcgtagttat cagcacccaa cgcccatatt tcgaaagcat
      361 tacatgcgac gaaatcacca gcaacagtga agtcatcatg ggggaaggcg ccgaaaccat
      421 caatggcaga gccatcgaca taagcggagt gcatgaccac atcagcggct tccacggcaa
      481 catcattggc gcagctacct gcatcatcca caaccatcac gaattcgtac acttcggtgt
      541 tggcggtcgt cacacccgtg aagtcatagg tgaaggttcc gttggcggct acaccaccat
      601 tcacggtcgc cacgttctgg cagttggcat tggtgtaggc agcgctgtct tcgcgggcat
      661 agatgtccac ataataggtg gtgccagcgg gaagggcacc gagggtccag tcgaaggtcg
      721 ctgtagccca cggggcggcc gcggcggcgg aaaggaattc cgagtcgcgg gtcagggtga
      781 tatcggctcc cgccacggtt gcggcggagg ccatgctgaa ctgggccaat acgaacacgg
      841 ccagcagcag tacggtgaag agtttcttgc tcattggggg tattactcct atatagggta
      901 gggtttgaac gcccatctcc acgagacgag tcgttcatta tttataacgg atgaaacccc
      961 aaaaagagac cggcaaatca ttgcaatttc gaaagttggt agtaattgtc cgaatcttgg
     1021 cagaaattgc ctctaaaaac gtcttttttc caccgcaaag taaactttgc ggtacgcatc
     1081 cctgacaaaa aagagagcgg acccgaaatc gggtccgctc cagacttgct tggaacaggt
     1141 gaaacttaga aggagccgta gtagtagctc aaggcgcggt agagcatctt ggcgaattcg
     1201 ccgcgggtca caggctgatc cggggagaag gtggtggcgg aggtgccatc ggcaataccc
     1261 cagccataag cctgaacgac aggttcagca tagaaggcgc caagggagac atcgctccag
     1321 gcagcggcgc ggtaaggttt gacagcatcg ttaaaggcga gcggcatcag gctgctgaac
     1381 aggtgagtgc cagggacgat gtttgcaatg ccgttatcca caatgttgtt catgacggtg
     1441 acgatctggc cgcgggtgag caggttgttc gggtcgaaca a
//
LOCUS       DAOQLC010000379         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_69379_length_1478_cov_20.089951, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000379 DAOQLC010000000
VERSION     DAOQLC010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69379_length_1478_cov_20.089951"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..1208
                     /locus_tag="PL000_22835"
     CDS             531..1208
                     /locus_tag="PL000_22835"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91771.1"
                     /translation="MTLHTWSRTLALHPHIHCLFTAGGEDKKGIWHDKGDYLFNVKNL
                     SRYFRGSYLYLIRQHAEQLGGAIEVNRLIHPLFDKNWHVYITEKYAHGNGVVRYLANY
                     VRGGPIKNSRIVAYDGQFVTFRYKDHRDNKNKTMRLPVSEFLRRFIMHLVPKRQRVIR
                     VLGIYAKRKKKNDALLPTVDNSILKALLYSEPENCPYCKMPMLLKANLSREEIAMYKP
                     PPKAVAA"
BASE COUNT          444 a          350 c          325 g          359 t
ORIGIN      
        1 cgttgaacga aatgcgtaga gcatgtcagg atttccccat ggcattctcc gtgatgccac
       61 cttttttcct cactccgtga gaattttctc cggttttctg gtatttatat tatatagcgg
      121 caacaattca tcagatttta gtggagcatt ttgacgaatt taacagaaat cgtcagatgc
      181 cagattatgc aagaaatgcg gtaagaagaa tgctgatctg taggacagat cagcttgggg
      241 ggcacgcatg gcgatgtacc aactgcaagc acacgaaggt tgcctataat tcatgtaaga
      301 acaggtcatg cccgcaatgt gggtgggtcg gtgtccaaga gtgggcaaag aaaacagccg
      361 ctaaactcaa gccggtagat cacttccaca taatattttc aataccccaa gagcttaatg
      421 gtttatggat cagagacacg aagctcatga ttcatctctt ctttaaagca gtcagacaaa
      481 cgctcgaatc tttctcacag aagcatattc acggggaacc tggatgcatt atgactttgc
      541 atacctggtc aagaacactc gctctacatc cgcatataca ttgccttttt acagcgggtg
      601 gagaagataa aaaaggcatc tggcatgata aaggcgacta tcttttcaac gtcaaaaatc
      661 tatcccgata cttcaggggc agttatctct atttaatcag gcagcatgcc gaacaactcg
      721 gtggggcaat cgaagtaaac agactcattc atccgctctt cgataagaat tggcacgtat
      781 acattacaga aaaatacgct catggaaacg gtgtagtccg atatctggca aattacgtca
      841 gaggtggacc catcaaaaac agccgtatcg tagcatatga cggacaattt gtaacattca
      901 gatacaaaga tcacagagac aataagaaca aaacgatgcg actgccggtt agcgagtttc
      961 tgcgacgctt cattatgcat ctggtaccaa aaagacagag agtcattcgt gttctgggta
     1021 tttatgccaa aagaaagaag aaaaacgatg cgctcctacc tacagtcgat aactccatat
     1081 tgaaagcgct tttatattca gaacctgaaa attgccccta ttgcaaaatg ccgatgctgt
     1141 taaaggcaaa cctatccaga gaagagatag ccatgtacaa acctccacca aaagccgtgg
     1201 cagcctaact acgcacttcg ttcaacagcg tttacctgct cgggctcgtc gcatacgctt
     1261 ccctcgccct cggcgctccg gcgacaaaga tcgcagcggc gcgcagctct tagagctgca
     1321 tttggcgcgc cgctgcgagt cgcctacggc acatttttga cgccaaatct ctaacaaaat
     1381 ttacgcattg acacaggcgt caaaaacgtc aggtaaacgc aagacgttga acgaaatgcg
     1441 tagagcatgt caggatttcc ccatggcatt ctccgtga
//
LOCUS       DAOQLC010000380         1455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_71580_length_1455_cov_23.785000, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000380 DAOQLC010000000
VERSION     DAOQLC010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71580_length_1455_cov_23.785000"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..966
                     /locus_tag="PL000_22840"
     CDS             76..966
                     /locus_tag="PL000_22840"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91772.1"
                     /translation="MKRSLRSHIWKFVIRKIYKKHMSVAEIRAQDASAAKFAGPPPKD
                     VQIERVNIDGIDAAWIRPSGAERTKTLLHIHGGGYVTGSIPSYLRMCILMAQMLNINV
                     LLPAYRLAPEHPFPAAMEDVLKIYRRLLNDGFQPKDIFISGDSAGGGLCVAAVIALRD
                     QKEALPAAVLCMSPWVDLTMQGQSHVTNPKSEAMLNAGTLREWALLYTNEKNFGSPLV
                     SPIFADFQKFPPLLIQVSSDEVLLDDARMLAEKARAGGADVTLKIWDGLWHVWQIVGD
                     AIPESRMAIEEIGEFIRAYR"
     gene            981..1319
                     /locus_tag="PL000_22845"
     CDS             981..1319
                     /locus_tag="PL000_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91773.1"
                     /translation="MQNFTDVKLEIFAPQEYALQLRHELAKIGVGKIGNYDHCFAIYP
                     VQGYYRPLEGANPFGGEVGKISSGVEYKIEVNCRRELVNEAIKVVKRIHPYEEPLINV
                     IPLANHLFIK"
BASE COUNT          371 a          339 c          387 g          358 t
ORIGIN      
        1 atcaacggga ttgtaaattt caaacttccg aagtcttaag gcttcggaag ttttttataa
       61 ccatgtgaga aaacaatgaa acgtagtttg agaagtcata tctggaaatt tgtcatccgc
      121 aaaatatata aaaaacacat gagcgtcgcc gagattcgcg cacaggatgc cagtgccgcc
      181 aaatttgcgg ggccgccgcc caaagatgtg cagatcgaga gggtcaacat cgatgggatc
      241 gacgccgcct ggatacgccc gtcaggtgcg gaaagaacaa aaacgctttt gcacattcac
      301 ggcggcgggt atgtgacagg ttccattcca tcctacttga gaatgtgcat cctgatggcg
      361 caaatgttga acataaatgt gcttttgccc gcatatcgcc ttgcgcctga gcatcccttc
      421 ccagcggcga tggaggatgt gctgaaaata taccgccgcc tattgaacga tgggtttcaa
      481 cccaaagaca tcttcatctc aggcgattca gcgggcggcg gattatgcgt tgcagcggtg
      541 attgcgcttc gggatcaaaa agaagcgctt cctgccgcag tgctgtgcat gtctccatgg
      601 gtcgacctca ccatgcaggg gcaatcgcat gtgacaaacc cgaaatccga agcaatgtta
      661 aacgcaggca ctttgcgcga atgggcattg ttgtatacaa acgaaaaaaa ctttggcagt
      721 ccgctggtct cgcccatttt tgcagacttt caaaaattcc cgccgctgtt gatccaggtc
      781 agcagtgatg aggttttgct cgacgatgcg cggatgcttg cagaaaaagc cagagctggc
      841 ggtgcggatg tcacgcttaa aatctgggat ggcttgtggc atgtctggca aattgttgga
      901 gacgcaatcc ctgagagccg catggcaatt gaagagatcg gcgagtttat ccgtgcttac
      961 cgctgattat ggaaggaagt atgcaaaact ttaccgatgt aaaacttgaa atattcgccc
     1021 cgcaggaata cgccttgcag cttcgccatg aactggcgaa gattggtgtt ggcaaaattg
     1081 ggaattacga tcattgtttt gccatttacc ctgtgcaggg atattaccgc ccgctcgaag
     1141 gcgcgaatcc ttttgggggt gaagtcggaa agatcagctc cggcgtcgag tacaagatcg
     1201 aggtcaattg caggcgagag ttggtcaacg aagcgatcaa ggtggtgaaa cgcattcacc
     1261 cgtatgaaga accgttgatc aatgtaattc cactggcgaa tcatcttttt ataaaatgaa
     1321 atcatccacc ctgcctttta tcctgcaagc gttgattgcc gcgttgtttt ttggggcgag
     1381 tgcgccgatc gcaaaattat tgctcggcga tgatatcgcg ccgatctttc tggcggcgtt
     1441 tctgtacctc ggcag
//
LOCUS       DAOQLC010000381         1451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_71971_length_1451_cov_13.373209, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000381 DAOQLC010000000
VERSION     DAOQLC010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71971_length_1451_cov_13.373209"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..696)
                     /locus_tag="PL000_22850"
     CDS             complement(154..696)
                     /locus_tag="PL000_22850"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC91774.1"
                     /translation="MDGIIIAVSRSADYTFSKPNETSIRLLAGLGVEGDIHSGEKVKH
                     RIRILRTPNQPNLRQVHLIHAELHDELRTQGFDVSPGQMGENITTRDIDLLGLPKGTR
                     LHLGKEAVVEVTGLRNPCEQLDGFQDGLLKACVAKDAQGKIIRKAGVMSIVLVGGEVF
                     PNDRIRIEFPPEPHQPLDVV"
     gene            complement(797..1276)
                     /gene="rraA"
                     /locus_tag="PL000_22855"
     CDS             complement(797..1276)
                     /gene="rraA"
                     /locus_tag="PL000_22855"
                     /inference="COORDINATES: protein motif:HMM:NF006875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HNC91775.1"
                     /translation="MKTTDLCDKYDTQLHIAEPIGFRSFGRRSEFHGRIETVKCHEDN
                     SFVRAALEKDGAGKVLVVDGGASLRCALLGDNMADLAYQNHWAGIIVYGCIRDSVAIS
                     QIGIGVLALGTHPRKSAKDKQGVAGVPVRFAGVVFTPGEFVYVDEDGVVTSREELLP"
BASE COUNT          360 a          405 c          327 g          359 t
ORIGIN      
        1 tcacatcgtg caaaaattcg atggaactct ggcgcttgat acagatgtga ttcaaagcgg
       61 tcagatcaaa gtaggcgatc cggtggagtt gatcgattaa ttcacccgtt cgggttggtg
      121 ccagtggaaa ggcctgacga gtctgaaggg tgcttaaacg acatccagcg gctgatgcgg
      181 ttctggcggg aactcaatcc tgattcggtc attcggaaac acttcgccac cgacaaggac
      241 aatgctcatc acacctgctt tgcgaatgat cttcccctgc gcatccttcg caacacaagc
      301 ctttagcagc ccatcctgaa aaccatccaa ttgttcacac gggttacgca gcccagtcac
      361 ttcgaccacg gcttcttttc ccagatgcag gcgtgtcccc tttggcaggc cgagcaagtc
      421 aatatcgcgg gtggtgatgt tctcccccat ctgcccgggg gagacatcga atccctgcgt
      481 tcgtaattca tcatgaagtt ctgcatggat taaatgcact tgtcgtaaat tgggctggtt
      541 gggtgttcgc aagatgcgga tgcgatgctt taccttctcc ccactatgaa tatccccttc
      601 aacacccaac cccgccagca ggcggatgct cgtttcattg ggcttgctga aggtgtaatc
      661 cgcacttcgg ctcactgcaa taatgattcc atccatttgg gctcctttta tctggcggta
      721 tacaacatat gtgtccagta agaggcggtt atacttcgcc agaggtgaac cttgtccgat
      781 caatacacag gttcgcttat ggcaaaagtt cctccctgct cgtcacgacg ccatcttcat
      841 ctacataaac aaactcacct ggagtaaaga ccacgcccgc aaaacgaaca ggaacaccag
      901 ccacgccctg tttatctttt gcgcttttcc tggggtgtgt gccaagcgca agaacgccaa
      961 tgcctatctg cgaaattgca accgagtcgc gaatacagcc atacacaata atgccagccc
     1021 agtggttttg atacgcaagg tcagccatgt tgtcgcccag caaggcgcaa cgcagcgaag
     1081 cacccccatc cactacaagg actttacctg cgccgtcttt ttccaacgca gcgcggacaa
     1141 agctgttatc ttcatggcat ttgaccgtct cgatccgccc gtggaactca cttcgtcttc
     1201 caaaactacg aaacccaatc ggctctgcta tatgcagttg agtatcatat ttatcgcaca
     1261 agtctgtcgt cttcattcac agctcctgat ggtaaattta caaaagtata cacgcttcac
     1321 aatcgggcgc attgacaaaa ctgcaaaata cgtgtatata cactcattat gctaaaagac
     1381 atgccaaagg aacagttata tgagttttcg cgccaagccg tgccgtgtgt gggattcaat
     1441 acccgccgtg c
//
LOCUS       DAOQLC010000382         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_72953_length_1441_cov_22.729437, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000382 DAOQLC010000000
VERSION     DAOQLC010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72953_length_1441_cov_22.729437"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..533
                     /locus_tag="PL000_22860"
     CDS             15..533
                     /locus_tag="PL000_22860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91776.1"
                     /translation="MQSLIAAVNQCCSQQNWYAALYIALTMPDICAKLDDPESKESGK
                     RYRSWFDTYLKPTYKSSFHGPDFHFLNAGDLWALRCSLLHEGTDEISHQRSREVLRKF
                     KFTTQGMHCGQMEDVLVLNVSAFCAEMIAAVELWLTVKQGEPKVLGRISEMAVIEQQG
                     FSPIPGVWIGGT"
     gene            685..1128
                     /locus_tag="PL000_22865"
     CDS             685..1128
                     /locus_tag="PL000_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91777.1"
                     /translation="MKKIELNGWYRLWILASGIWATCIGFYLFDGYNAAAEERVAKYE
                     LSNPVHTSEPSKKEIRIVFSKSLPDDQIRKIAEEELIPKLMEGKFDNLPLEYRKPYEE
                     FLANEKEETFVSFAKLGVLIPILLLIIGQAVAWVINGFRGSEKKT"
BASE COUNT          396 a          316 c          364 g          365 t
ORIGIN      
        1 gttaggcatc tgaaatgcaa agcctcatcg ccgccgtcaa tcagtgttgt tcgcagcaga
       61 attggtatgc ggcgctctac attgcgctaa caatgcccga tatatgcgcc aagttggacg
      121 accccgagtc aaaggagtcg ggtaagcgat atcggtcttg gtttgatacg tacttgaagc
      181 cgacatacaa gagctccttt cacggtcctg attttcattt cctcaatgcc ggcgaccttt
      241 gggcactacg atgctctctg cttcacgagg gtacagacga gatatcgcac caaagatcac
      301 gcgaggtctt gcgtaagttc aagttcacaa ctcaagggat gcattgtgga caaatggaag
      361 atgttcttgt tctaaacgta tcggctttct gtgcagagat gattgcagcc gttgaactct
      421 ggctcaccgt aaagcaagga gagcccaaag ttttgggacg catctctgaa atggcagtta
      481 ttgagcagca aggcttcagc ccaatcccag gagtctggat tggcggcact tagtttctgg
      541 agcgcgcaga tgcctaacca ttcgctcaac cggacccatt gcggcgtgcg gctaaaggcc
      601 cgccatttca ttctgggcct ttagccacac accacaacgg tccggttagc tcaaacgtta
      661 ggtctaatcc acttcgagcg ccatatgaaa aaaatagaac taaatggatg gtataggctt
      721 tggattttag catctggcat atgggctacc tgcatcggat tttatctatt tgatggttac
      781 aatgcagcgg cggaagaaag agtcgccaaa tatgagctaa gtaatccagt gcatacgtca
      841 gagccaagca aaaaggaaat acgaatagta ttttcaaaat cacttccgga tgatcaaatc
      901 cgaaaaatag cagaagaaga gctaatacca aaactgatgg agggtaaatt tgacaattta
      961 cccttggagt atcgcaagcc atatgaagaa tttttagcca acgaaaagga agaaacattt
     1021 gtaagcttcg caaaattagg agttttgatc cccattcttc tattaattat tggccaagct
     1081 gtagcatggg taattaatgg cttcaggggg agcgagaaaa agacctaact gtcaagtccc
     1141 gtatgatcat aaggtttgtg atgaaacaga tcggggtgag tggtgtacca ctgattcatg
     1201 gtctgcatgg gagttttacc tcaatggaga aatggggaca gaccacggtt ttctgttttt
     1261 ggctattgat cgggcaagtg aatcttgagc agtaaaccgt ggtctgtccc catttcccct
     1321 gtccccattt cccttcgtaa cggttgacga tgttcgggct gtgaacgcaa aaagcccgca
     1381 atgacacggg cttgtggaat gattcgcact ggagcgaaag gatgtctggt gccgagagtc
     1441 g
//
LOCUS       DAOQLC010000383         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_73267_length_1438_cov_20.187997, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000383 DAOQLC010000000
VERSION     DAOQLC010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73267_length_1438_cov_20.187997"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1030)
                     /locus_tag="PL000_22870"
     CDS             complement(275..1030)
                     /locus_tag="PL000_22870"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC91778.1"
                     /translation="MNIVVAIKHIPSIADDLPVKGNNVDFDSVDFVLNEWDEHSIEQA
                     VLIKEAVGGTVTVIGVDLIGELDNALHLALAKGADKVVKITADDPAADSHMQAKWLAE
                     AIKGMGANMVFAGVQASNDLDGQIGPMIAAYLDMPYIGGVSSVEANGDSATVNKEFAG
                     GAGAKYSVSMPFVAGVQSAPKPPRYAPVSKVRQMAQSATVEKVAPSGDASSGLAFKKM
                     APPVVTGHAEMIEGSSKEVAKKIVELLKSKGLA"
     gene            complement(1137..>1438)
                     /locus_tag="PL000_22875"
     CDS             complement(1137..>1438)
                     /locus_tag="PL000_22875"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HNC91779.1"
                     /translation="LLEAIPGVELVEMQRNRENGYCCGGGGGGMWMDSFTAKHTTMRL
                     SEKRAMEAASTGANVLAVACPFEVSRFTDAVKSTGNEHVDVLDILELLDRSMRGE"
BASE COUNT          314 a          448 c          331 g          345 t
ORIGIN      
        1 ggatcgtgga atcagacgcg aacacattgg acgaaacccc gcctccgact acgaccgcaa
       61 ctgcttgccc tcccaaagcg gacgccaatt ctttggcttt tcctaccatt tcaaacgaca
      121 catcagagaa cttgccatcc atatgttcgg tgattacaaa gacatcgtta ctcatgtttg
      181 ctccttcgga gagagaatag ataattagat attagagatt agtcgtcttt actactcact
      241 attctctact ctctaatatc taatttctgc tcttctacgc caatcccttg ctcttcaaca
      301 actccacgat cttcttcgcc acttccttcg acgaaccttc gatcatctcg gcatgacccg
      361 tcaccacggg cggagccatc ttcttgaacg cgagtcccga agacgcgtca cccgacggag
      421 cgaccttctc caccgtggca gattgcgcca tctggcggac tttgctcacg ggcgcatagc
      481 gcggcggctt cggcgctgac tgcactcccg ccacgaacgg catggacaca gaatacttcg
      541 cgcccgcccc gcccgcaaat tccttgttga ccgtggcaga gtcgccgttg gcttcaacag
      601 aactcacgcc gccgatgtag ggcatatcca aatacgccgc gatcatcgga ccgatctgtc
      661 catcaaggtc attcgacgcc tgcacacccg cgaagaccat gttcgcgccc atgcctttga
      721 tcgcttccgc caaccatttg gcttgcatgt gcgaatccgc ggcgggatcg tcagcggtga
      781 tcttgaccac tttgtcggcg ccctttgcca gcgcgagatg aagcgcgttg tccaactccc
      841 cgatcaagtc cacgccgatc acggtcaccg tcccaccgac ggcttccttg atcaacacgg
      901 cttgctcgat ggaatgctcg tcccattcat tgaggacaaa atccaccgag tcgaaatcca
      961 cgttattgcc tttgacgggc aggtcatcgg caatgctggg gatatgtttg atggctacta
     1021 cgatattcat tcagttctcc tgtgtttcgg tttcaggtat caagttacag gtttcaagtt
     1081 gaaggttgag agttatcgaa cctgcaacct gtaaccttca acttttaact caatagttac
     1141 tcacccctca tcgacctatc caacaactcc aaaatatcca acacatccac atgctcattg
     1201 cccgtggact tgaccgcgtc ggtgaagcgt gagacttcga aggggcaagc cactgccagc
     1261 acgttcgcgc ccgtggacgc ggcttccatt gcgcgttttt cagagaggcg catggtggtg
     1321 tgtttggcgg tgaaggagtc catccacatg ccgccgccac caccgccgca gcagtaaccg
     1381 ttttcgcggt tgcgctgcat ttcaaccaac tccacgccag ggatggcttc gagcaatt
//
LOCUS       DAOQLC010000384         1437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_73370_length_1437_cov_16.842258, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000384 DAOQLC010000000
VERSION     DAOQLC010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73370_length_1437_cov_16.842258"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PL000_22880"
     CDS             <1..1307
                     /locus_tag="PL000_22880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC91780.1"
                     /translation="LTLALIIAMLAFVLASYWLALRGHVLEASLLFVSELWVVFSLAI
                     LLTGRFNSSYVSLHLAIIVMAGVLLGMRPTIVFSCLSVLFGFVLAFLESTGYPLVRYF
                     PVQPLAGWFTWVLSIILILTPLTPTIQNIAYSIVALREKQRFIESILTATPNTIHLFD
                     LKERRSVFSSRSLISALGYMPERSQSIDNDDLLHPEDRTQRSNLYKLAETAKDGETLT
                     VEYRMRTSSNEWRWFLERDMVFHRGVDGKPQQLIGIIMDITERKQLEAELERLATVDS
                     LTGVLNRRKLSSLATVELERARRYGHSTSAIMLDIDYFKKINDTYGHAAGDEVLAGLA
                     QLLTRIARTSDLVARYGGEEFVLILPETNITDASDFAERIRRTVADTPFVVEGQTIRF
                     TVSLGVASSEQIEQDFESLLKETDRQLYRAKELGRNQVASRR"
BASE COUNT          348 a          341 c          363 g          385 t
ORIGIN      
        1 ctcttacact tgctcttatc attgccatgc tggcttttgt gttggcttct tattggctgg
       61 cgttacgtgg ccacgttttg gaagccagtc ttttattcgt gagcgagttg tgggtcgtat
      121 tctcccttgc gatcttattg acggggagat tcaactcatc ttatgtgtcc ctgcatctgg
      181 caattattgt catggcaggg gtgcttctgg ggatgcgccc cactattgtt tttagctgct
      241 tgagcgttct ttttgggttt gtactggcat tcctggaatc tacaggctat ccccttgttc
      301 ggtatttccc cgtgcaacca ttggctggct ggttcacgtg ggtgctttcc attatcctga
      361 tattaactcc cctaactccc acaatacaga atattgccta ctcgatcgtt gctttgcgcg
      421 aaaagcagcg cttcatcgaa agtattctaa ctgccacacc aaataccatt catctttttg
      481 acttgaaaga acggcgcagt gtattttcaa gtcgcagttt gatttcagca ctgggttata
      541 tgccagaacg gtcccaatcg atagataatg atgatctgct tcatcctgaa gatcgtacac
      601 agcgatcgaa tctatataaa ctggcggaaa ccgcaaaaga tggggagaca ttgacggttg
      661 aataccgcat gcgcaccagc agtaatgaat ggcgctggtt tctggaaagg gatatggtat
      721 tccatcgcgg agtggacggt aagccgcaac aactgatcgg aatcatcatg gacatcaccg
      781 aacgcaagca gttggaagcc gaacttgaac ggctggcaac cgtcgattca ttaacaggag
      841 tgcttaatcg ccgcaaactt agctctttgg caacggtcga acttgaacgc gcccgccgct
      901 atgggcattc cacatccgcc attatgttgg atatcgacta cttcaagaag atcaacgaca
      961 cctatgggca tgctgctggt gatgaagtgc tggcagggct ggcacaattg ttgaccagaa
     1021 tagcccgcac cagtgacttg gtggcacgct acggcggcga agaatttgta ctcatcctgc
     1081 ctgagacaaa cataaccgat gcgagcgact ttgccgaacg tattcgccga actgtcgcag
     1141 ataccccatt tgttgtggaa ggacaaacca tccgttttac ggtcagcctg ggtgtggcaa
     1201 gtagtgagca gattgagcag gattttgagt cgttgctcaa agaaacagat cgtcaactgt
     1261 atcgagccaa ggagttgggg cgaaaccagg ttgcatctcg aagatgaaat tttgtgcctg
     1321 cgtcatcgct tgcgaaggcg atcccctgac gaagatgtac atcgaaggct gatccgctca
     1381 cgaaacgatg atgaccgaaa aattcggcta gaatatgctt ccgcatcggg tgaaata
//
LOCUS       DAOQLC010000385         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_73799_length_1433_cov_17.040639, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000385 DAOQLC010000000
VERSION     DAOQLC010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73799_length_1433_cov_17.040639"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..938)
                     /locus_tag="PL000_22885"
     CDS             complement(150..938)
                     /locus_tag="PL000_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91781.1"
                     /translation="MSLTDELDELIESAKLLGRTEARKENGCYTPHDSRFRTSLHESI
                     SKHRSAIEAAVLQAQPTDQRLLNLLCRIHRDGGHYIAKHGLDKAVEDADIRVAEQNAA
                     LDAQAKQEPVAWCFVQNGFTHYTDDKRDWYDAVGVEEVTPLYAAPVVPPFEDFFPDAC
                     RLALELECLLLSCNDNAAVSKWWDSAHEALEQHRELVAAPVVQPDRVLVPREPTEAML
                     DAAMNRYKHVSPEAKARYTQMHRENFRCDYRAMIAAAEKEISPK"
     gene            complement(935..1183)
                     /locus_tag="PL000_22890"
     CDS             complement(935..1183)
                     /locus_tag="PL000_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91782.1"
                     /translation="MSKCREAFEELYGKQELVRSITNPEFYADFEVTRAWRYWQAAWN
                     ARGKVDAEICRNRIGPLDTAFDVEARACAEAIEQEIEK"
     gene            complement(1195..>1433)
                     /locus_tag="PL000_22895"
     CDS             complement(1195..>1433)
                     /locus_tag="PL000_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91783.1"
                     /translation="SRCTPCAKADEGGREMSKHRILFEGKYIYAFPAHGNPLTTKHKS
                     LAIIFGETETIRMVMQYKHLGYVARAVPAITVKI"
BASE COUNT          285 a          396 c          393 g          359 t
ORIGIN      
        1 ggtgaaccag attccgattt cgaaagctgg cccccagccg ggggtaagcc cgtagagggg
       61 aaggatgagg tagttggcgg tgactgagat cccgaagccg atgaggacgt tggcccaggc
      121 ctcgatgaag gagtggaggg cgcgttggct catttcgggg agatctcctt ctctgcggcg
      181 gcgatcatgg cgcggtagtc acaacggaag ttctcccggt gcatctgggt gtagcgggcc
      241 tttgcttccg ggctgacgtg cttatacctg ttcatagccg cgtcaagcat tgcttcagtc
      301 ggctccctcg gcaccagcac cctgtcaggc tgcacgacag gggcggcgac cagttctctg
      361 tgttgctcca acgcctcatg cgccgaatcc caccacttgc ttacggcggc gttgtcattg
      421 catgacagga gcaggcattc aagctccagt gccaaccggc aagcatcagg gaagaagtcc
      481 tcgaaaggcg gaacgactgg cgcggcgtag agcggagtca cttcctcgac ccctacagcg
      541 tcgtaccaat cgcgcttgtc atcggtgtag tgcgtgaagc cgttctgcac aaaacaccac
      601 gccaccggct cctgcttcgc ttgagcatca agggcagcat tctgttctgc tacacgtatg
      661 tctgcatcct ctactgcctt atctaaccca tgcttggcaa tgtaatgccc gccgtctcga
      721 tggatacgac aaagcagatt tagtagacgt tggtcagtgg gttgagcctg aagcactgcg
      781 gcttcgatgg cgctgcggtg tttgctgatt gactcgtgca gggaagtgcg gaagcggcta
      841 tcgtgtggcg tatagcagcc gttttcttta cgcgcttcgg ttctgccaag tagctttgcg
      901 ctttcaatca attcatcaag ttcgtcagtc aggctcattt ctcaatctcc tgttcgatgg
      961 cttcggcgca cgctctagct tcaacatcga aagcggtatc aagcggccca attctgttgc
     1021 ggcaaatctc cgcatccacc ttgccgcgag cgttccatgc ggcttgccag tagcgccatg
     1081 ctctagtcac ttcgaagtca gcgtagaact cagggttggt aatgctacgg acaagctctt
     1141 gcttgccgta tagctcctca aacgcttctc gacacttgct catttcactt ctccctatat
     1201 ttttacagtg atcgccggaa cagctctagc aacatatcca agatgcttgt actgcatgac
     1261 catacggata gtttcagttt caccaaagat aatggcgagc gatttgtgct tagtagtaag
     1321 cgggttgccg tgtgcgggaa aggcgtaaat gtatttgcct tcaaatagaa tccgatgttt
     1381 gctcatttcc cttcctcctt cgtctgcttt cgcgcacggt gtgcagcggc ttc
//
LOCUS       DAOQLC010000386         1426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_74515_length_1426_cov_25.889132, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000386 DAOQLC010000000
VERSION     DAOQLC010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74515_length_1426_cov_25.889132"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..625)
                     /locus_tag="PL000_22900"
     CDS             complement(161..625)
                     /locus_tag="PL000_22900"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC91784.1"
                     /translation="MCFSQIHGPMGRKFKMIQLPAEERAYLEQYVKQGRSRAVEQARS
                     RILLLSDRGETARQIATLLDWHYGSVMQILRRYELGGLERALFDAPRSGAPRKLTPEL
                     EARVSALACSEAPDGHVRWTAQMLADEIVRLEAVESISASSIGTVLKKGVKT"
     gene            complement(1077..1358)
                     /locus_tag="PL000_22905"
     CDS             complement(1077..1358)
                     /locus_tag="PL000_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91785.1"
                     /translation="MLNELRALLDRNLEAARSGQKTTADGTGTAFELYCNEVAYTNNI
                     ILVHAAGQPCELYTTLDNPNFIKTTDPRLCNQAKDWLDHIQQASVQVYR"
BASE COUNT          299 a          408 c          351 g          368 t
ORIGIN      
        1 ctagtgttct gtccaaatag ttctgtcaaa ttttggctgc ggctttttgt cccatacttc
       61 agccattttt tcgcccgatg cgctgcatcg aactcaaaaa tcgcctcgcc tgatccaaaa
      121 atccactacc caaaattcaa cgaactactt gtacagaaca ctaagttttt acaccttttt
      181 ttaaaaccgt gcctatgctg ctggcactga tgctttccac cgcctccagc cgcacgatct
      241 cgtctgccaa catctgcgcc gtccagcgga catggccatc gggggcttcg ctgcaggcaa
      301 gcgcactcac ccgcgcctcc agttcagggg tcaatttgcg cggcgcaccg cttcgggggg
      361 catcgaacaa ggcccgttcc aggccgccca gttcataacg cctcaaaatt tgcatgacag
      421 agccatagtg ccaatccagc aaagtagcga tttgtcgtgc cgtctcgccg cggtcgctga
      481 gcaacagtat ccggctacga gcctgttcca cggctcgcga cctcccctgt ttgacgtact
      541 gttccaaata cgcccgttct tcggcaggca actgaatcat cttgaatttt cttcccattg
      601 gaccatgaat ttgtgaaaag cacaaattta ctactctcct catttttcaa tgaatctttt
      661 acgtagaaca cttagcctgc tctcatttcg ctcaaaaaag gtcggctttc gcccatgttt
      721 cgctgcctct ctcgtccgac gcggggcatc gaactccaaa aggcggctca tctggacaaa
      781 acccgcctgc ttttttgatt cgaaaccgct gggctaagca gttgccaagt tttcacggtt
      841 ttggcatgtt gggttcccca cgcagccatc ggctacggga ggaacccaac atgcgcctgc
      901 tcgctcgcag cctatagcat ggggctccaa ctgcgccttc cgccctgctt ggtgttgtca
      961 ccaagcaggg cggaaatctt agacctgctc ttatcccttc agccgtgctt ctacgatcgc
     1021 tactttttca tacatccgct cgaagagggt gtgccgatgc tgctcgccgc ggtcgcttac
     1081 ctgtacacct gtacagaggc ctgctggatg tggtcgagcc aatccttggc ttgattgcac
     1141 agccgagggt cagtagtctt gatgaagttg gggttgtcca gcgtggtgta gagctcgcag
     1201 ggttggccgg cagcatgcac caggatgatg ttattggtat aggccacttc attgcagtac
     1261 agctcgaagg cggtgccagt gccgtcagca gtagtctttt gacctgagcg agcggcctct
     1321 aagttgcgat caagcagtgc gcgaagttca ttgagcaaga tgctgatggt atgcttagta
     1381 gtcagtcaat cgataagagg cgagttgatt ttcaaatgac ggggta
//
LOCUS       DAOQLC010000387         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_74617_length_1425_cov_25.343796, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000387 DAOQLC010000000
VERSION     DAOQLC010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74617_length_1425_cov_25.343796"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..1338)
                     /locus_tag="PL000_22910"
     CDS             complement(247..1338)
                     /locus_tag="PL000_22910"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNC91786.1"
                     /translation="MYLKKVIQQMRVQLKSILPVFHKAQVENLILMVVGMVYAQSVNL
                     PKVATKMPLKIQIEGRVQRLERLLANNKFEPLEVMKPVAIRVLKQVSRRSRKVRLVMD
                     RSMINDTINVLHVAVVFHGRALPLGWVEVPHEGMSDLALQQELLRWVAGCFPKRVSVS
                     LVADREFHSIWLATWIATSLKWSFGLRIKGNTKIEIDGQMVDASTLAIKGACTWHESV
                     KVTTDKAATYRVNFFTEWVVTEDEPWLIITNFSHPSKVEPAYCRRFGIEEMFSDLKSR
                     GLNLEDTRLTDPKRLLRLLAATAIAYLWIMEVGLTAVANGWRKYIDNRGQTSTLSLCQ
                     IGLRWIEDRLTQGLAPPRFRFRFHLLPDS"
BASE COUNT          323 a          428 c          328 g          346 t
ORIGIN      
        1 ccgggttccg ggttccgggt tccgggttcc gggttccggg ttccgggttt tcgaactttg
       61 gtcctttgtg tccttttcgt cctttttgtc ttttccccga gccctggttt tacagcaggc
      121 cgtaaacgac ctgcctcaat tcaaccaccg ctacgcggcg aaaactataa tcgacgaaga
      181 ttcgaaaacc ctgaaccctg aaccctgaac cctgaaccct gaacccttga ctgggggtcg
      241 cttttttcag gaatccggga gcaggtggaa gcgaaagcga aaacgaggag gtgccaaccc
      301 ctgagtcaag cgatcctcga tccagcgcag gccaatttga cataaactga gggtggaagt
      361 ctgtccgcga ttatcaatgt atttgcgcca gccattggcc acggcggtca agccaacttc
      421 cataatccat aaataagcaa tcgcggtggc ggcaagcaac ctcaggaggc gtttggggtc
      481 agtcaggcgg gtatcttcga gattgaggcc acgacttttg agatcggaaa acatctcttc
      541 aatgccgaaa cggcggcaat acgcgggttc gaccttggag gggtgggaaa aattggtgat
      601 gatgagccag ggttcatctt cggtgaccac ccactcggta aagaaattga cccgataggt
      661 cgccgctttg tcggtggtga ctttgactga ttcgtgccag gtgcaggccc ctttgatcgc
      721 cagggtcgag gcatccacca tctgaccatc gatttcaatt ttggtgttgc ccttgatccg
      781 caagccgaat gaccacttca agctcgtcgc aatccaggtc gccagccaga tcgaatgaaa
      841 ttcgcggtcc gccaccagtg acacgctcac ccttttgggg aaacaccccg ccacccacct
      901 cagcaattcc tgctgcaatg ccagatccga catcccctcg tgtggcactt ccacccaccc
      961 gagcggcagc gcccgcccgt ggaacaccac tgccacatgc aacacattga tcgtatcgtt
     1021 gatcattgac cgatccatca ccaaccttac cttccgactc cgccgactca cctgcttcag
     1081 cactcggatc gccaccggtt tcatcacctc cagcggctcg aacttgttat tcgccagcaa
     1141 ccgttccaat cgctgcaccc gtccctcaat ttggatcttg agcggcattt tcgtcgccac
     1201 ttttggcaag ttcacacttt gcgcgtacac cattcccacc accatcagaa tcaagttttc
     1261 cacctgcgct ttatgaaaca ccggcaggat gcttttcaat tgcacccgca tttgttgtat
     1321 aactttttta agatacattc ttcaccttta ttattgctat ataatgactt acaaggttta
     1381 gaatgtatca cacctctcct tccaaaaaaa gcgaccccca gtcaa
//
LOCUS       DAOQLC010000388         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_76215_length_1410_cov_12.526937, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000388 DAOQLC010000000
VERSION     DAOQLC010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76215_length_1410_cov_12.526937"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..835)
                     /locus_tag="PL000_22915"
     CDS             complement(32..835)
                     /locus_tag="PL000_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91787.1"
                     /translation="MLREFDFSIADELKGADILTSTREARGTTLSMRGMDEKFKGGWP
                     HDPAIIAERILAHFLIRFAASSCPPMTLESPGHAPIDLHKFFETTVLTHIEEKFFDVL
                     GHTFALQAYRHRDGRSRHDLHLCANGREVTTSKLRDLLPELPEKLLDANQEPYTLIVL
                     VTGEYLDDHANQERTRISFQSDDELDLDETLVSRQALNRGITDTLRPLLADDLQSTNE
                     EKRSQIEKFVEQAPEYRALTPYPYTQLKDLAVSCRFFEKVAGWAGLRRN"
     gene            complement(889..1215)
                     /locus_tag="PL000_22920"
     CDS             complement(889..1215)
                     /locus_tag="PL000_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91788.1"
                     /translation="MLANIKGLVDRLELSQAKAMMPLFEAISNAIDAIEEHRDGFASH
                     AIQIRLVAAHDLAHQGGDEVFVVDGFDIRDDELGLAMTTLLRFKRRIPCQKSKLAVKV
                     LAVLPS"
BASE COUNT          374 a          367 c          326 g          343 t
ORIGIN      
        1 ttcagtcttc ggtcccccag atcaacttcc ttcagttcct ccgcagccca gcccagcctg
       61 ccaccttctc aaaaaatcgg cagcttacag ccagatcctt aagttgtgta taaggatatg
      121 gagtaagggc acgatactca ggtgcctgct ccacaaattt ttcgatctgc gaccgttttt
      181 cctcatttgt cgattgcagg tcatctgcga ggagagggcg cagtgtgtct gtgatccctc
      241 gattgagcgc ttgtcgagaa acaagggtct catcgagatc gagttcatcg tctgactgga
      301 aagaaattcg ggtgcgctct tgattggcat ggtcgtcgag gtattcacca gtaaccaaaa
      361 caatcaacgt gtatggctct tggtttgcgt ccagtaattt ttcaggcaat tcaggcaaca
      421 agtcacgtaa tttactagtg gtgacttcgc gcccattagc gcacaaatga aggtcatgcc
      481 gtgaacggcc atcccggtgt cgataggctt gcagagcaaa ggtatgccca agcacgtcaa
      541 aaaatttttc ctcaatgtga gtcaagaccg ttgtttcaaa aaacttgtgc aaatcaatag
      601 gcgcatgccc cggcgactcc aaggtcatag gaggacagga actcgcagca aaacgaatca
      661 aaaaatgtgc aaggattcgc tcagcaatga ttgcagggtc atgcggccaa ccacctttga
      721 atttttcatc cattccccgc atggataggg tcgtaccacg agcctctctc gtggatgtga
      781 ggatgtcagc gccctttaac tcgtcagcga tggagaaatc aaattcacgc agcaaggttt
      841 caccgtgccg ctgaaatacg ctacggatat ggacgttgga aaagactttc aagaaggtaa
      901 aacggccaac acctttaccg ccaactttga cttttgacag ggtatgcgcc tcttgaaacg
      961 aagcaaggtt gtcatcgcca aacccaactc gtcatcacga atatcaaagc catcaactac
     1021 gaacacttcg tcaccgccct gatgggcgag gtcgtgcgca gcgacaagac gaatttgaat
     1081 tgcgtgactt gcgaaaccat ctctatgctc ttcgatagcg tctatggcat tagatatggc
     1141 ctcaaacaaa ggcatcatgg ccttagcctg tgatagttct aggcgatcaa caagtccttt
     1201 gatgtttgca agcatgattc ctccgtgggc tatatcgtat tcggtctttg ccccagccta
     1261 aaccagaaaa cttaccccca actgaacccc ggaaaacaaa aaagcccaag caacttgctt
     1321 gggctttctg taacctgttg acttacaaca gaatttttgg ctccccgacc tgggctcgaa
     1381 ccagggacct acggattaac agtccgacgc
//
LOCUS       DAOQLC010000389         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_76434_length_1408_cov_10.830746, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000389 DAOQLC010000000
VERSION     DAOQLC010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76434_length_1408_cov_10.830746"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1194
                     /locus_tag="PL000_22925"
     CDS             151..1194
                     /locus_tag="PL000_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91789.1"
                     /translation="MRVISRPSTARCTLAMYVSFLLSEPHSPTGNRLAEVMEISHDSV
                     NRFLLREDYTPRDLFEEVRSGLTLKGGTVSVDDSVLDKPYSQLMAFVGYFWSGKHHGV
                     VKGINLMTLYYTDPHGRHQPINYRIYDKTANKTKNDYFQEMLVEVLAWGLQPAWVTGD
                     SGYSSTANLKMIKNHHQGFLFALESNRLVAIEPGRWLQVQQLAIPEHGLRVHLRGFGT
                     VKVFRKWLKNQPRHYALYPPNDEEWDAVDRPAFLKLHDQHWHIEQYHRALKQACHIEH
                     FQVRQPTAIRNHLFAAICGYVQLQRLQATDLLGNCYRLQRELFNEVMATFIHAFIPNL
                     EHLNPQLQPPVNA"
     gene            complement(1198..>1408)
                     /locus_tag="PL000_22930"
     CDS             complement(1198..>1408)
                     /locus_tag="PL000_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91790.1"
                     /translation="HNREVDRDDAEQRQWNQRQTPENIGKQGFTGSTSFRYRSSHRPC
                     CSFRCCRAQIMVPASPLCQATLIFT"
BASE COUNT          331 a          364 c          375 g          338 t
ORIGIN      
        1 cgcccgcagc ggggtttgca ggtcaacccc ggtgagggcg agttcggcct tcagggaggc
       61 actgatatag ccttttagaa cttacgcatt gacacgcatt gtatagtgtg cttggcaaag
      121 atgagcatga tccatgaagg tttaccgttg atgcgagtca tcagtcgtcc atcgaccgcg
      181 cgttgcactt tggcgatgta tgtttctttt ttgttgagcg agccgcattc tcccacgggg
      241 aatcgcttgg cggaggtcat ggagatcagc catgacagcg tcaatcggtt tttgctccgc
      301 gaagactaca ccccccgcga tttgttcgag gaagtccgct ccggcttgac gctgaaggga
      361 ggtacggtca gtgtcgacga cagtgtgtta gataaaccgt acagccaact gatggccttc
      421 gtgggttatt tctggtcggg aaaacatcat ggtgtcgtga aggggatcaa tctgatgacg
      481 ttgtattaca ccgatccgca cggtcgccat caaccgatca attaccgaat ttacgataaa
      541 accgcgaaca agaccaaaaa cgactatttt caggaaatgc tcgtggaagt gctggcttgg
      601 ggactccagc cggcctgggt caccggcgat agcgggtaca gtagcaccgc gaatctcaag
      661 atgattaaaa accatcatca gggatttctg tttgcactgg aaagcaaccg tctggttgcc
      721 atcgaaccag gtcgctggct ccaggtgcaa cagttggcga ttcccgaaca cggcttgcgc
      781 gtgcacctcc ggggattcgg gacggtcaag gtgtttcgga agtggttgaa aaaccaacca
      841 cgccattatg ccttgtatcc accgaatgac gaagagtggg acgctgttga tcgtcctgcc
      901 tttctcaagc tccatgacca gcactggcac atcgagcagt atcatcgggc actcaaacaa
      961 gcgtgtcaca tcgagcattt tcaggtccgc caaccgaccg ccattcgaaa ccatctcttt
     1021 gcggcgatct gtggctatgt ccaattgcag cggctacagg ctaccgacct gcttggcaat
     1081 tgttatcgtt tgcaacgtga attgtttaat gaggtgatgg ccacgtttat ccacgccttc
     1141 atccctaact tggaacacct caacccacaa cttcaacccc ctgtcaatgc gtaagtccta
     1201 ggtaaaaatt aaagttgcct gacacagagg acttgctggg accatgattt gggcgcgaca
     1261 acatcgaaat gaacagcaag ggcgatgact tgagcgatag cggaatgaag tggagcctgt
     1321 aaacccttgt ttgcctatat tctccggcgt ctggcgctga ttccactgac gttgctcggc
     1381 atcatcgcga tcaacttcgc gattgtgc
//
LOCUS       DAOQLC010000390         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_76891_length_1404_cov_22.129726, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000390 DAOQLC010000000
VERSION     DAOQLC010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76891_length_1404_cov_22.129726"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..702
                     /locus_tag="PL000_22935"
     CDS             25..702
                     /locus_tag="PL000_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91791.1"
                     /translation="MTSKEFVELIGHVAWPVAVLLGLFWMKGALRSFLAAVAARVEDR
                     QTNVAITKDGLEIKALEVVRPELVKLASVSANPGQNLPEGGAIAGDAEPEFETTRDAI
                     YKNNRRLFLVHILEPTKDRTQLYDIFIYIKRHKDAPIDDVVKTEFFFGKFWGNRIFEG
                     TRADGTYGVRTSAYGEFLCTCRVQFSDGESVLLQRYIDFDSGKYLKEVIEEANKSFQR
                     TAASGVR"
     gene            738..1301
                     /locus_tag="PL000_22940"
     CDS             738..1301
                     /locus_tag="PL000_22940"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC91792.1"
                     /translation="MFRKLKAVAVLLAFPALALAQVPFPQIQQPTQTFSFEEKDWSVE
                     PTATVKRAPYHAPTPTAIPGARVIKTLELKALLDANKNVLVIDVLDTKSRKSIPGAFW
                     MPGAGDGQFFGAEKSRLAAALEKLSSGDKARPLVFLCLSSECWLSYNASLHALEAGYK
                     DVIWYRGGTNSWTGASLEWTKPERIDW"
BASE COUNT          330 a          377 c          381 g          316 t
ORIGIN      
        1 cgttagcctc atcgaaaggg catcatgacc tcaaaggaat tcgtcgaact gatcggtcac
       61 gttgcatggc cagtagctgt cttgctcggc ctgttttgga tgaagggcgc tttgcgatca
      121 tttctggccg cggtggctgc tcgcgtcgag gaccggcaaa caaacgtagc cattacaaaa
      181 gatggcctag aaattaaagc gcttgaagtt gttcgccctg aacttgtgaa gcttgcctcc
      241 gtttctgcga atccgggtca aaaccttcca gaggggggcg ctattgcagg ggatgccgag
      301 cctgaatttg agacgacacg ggacgccatt tacaaaaaca ataggcgcct cttccttgtt
      361 catatcctag agccaaccaa ggacagaaca cagctgtacg atatatttat atatatcaaa
      421 cggcacaaag acgctccgat agatgatgta gttaaaacgg agttcttctt cggtaagttc
      481 tggggaaata ggatttttga gggaactcgt gccgatggaa cctatggcgt gcgcacctcc
      541 gcgtacggtg aatttctttg cacctgtcga gtgcagtttt cagatgggga gtcggtactc
      601 cttcagagat atattgactt cgactctgga aagtacctga aagaggtcat agaagaggct
      661 aacaagtcct tccagcggac cgccgcaagc ggcgtccgct gaagtcaaac gttgggcctg
      721 ttaatcggag gtagtccatg tttcgaaagc ttaaagcggt tgctgtcttg ctagcatttc
      781 ccgcgcttgc tctcgcgcag gtaccgtttc cacagattca acagcccaca cagacgtttt
      841 cgttcgagga aaaggattgg agcgtcgagc caactgcgac cgtcaagaga gcgccgtacc
      901 atgcgccaac tccaacggct atcccagggg ctcgcgtcat aaagacactt gaactcaagg
      961 cactccttga cgctaacaag aacgtcttgg tcattgatgt cctcgacaca aagagtcgaa
     1021 agtcgatccc cggtgcattt tggatgcccg gagccggcga cgggcagttc ttcggtgcag
     1081 agaaaagcag acttgccgcc gcactggaaa agctctccag cggcgacaag gcacgcccac
     1141 tcgtttttct gtgcctgagc tccgaatgct ggctttccta caacgcctcc ctgcatgcgt
     1201 tggaggctgg ctacaaggac gtaatttggt accgcggtgg cactaattcc tggactggcg
     1261 ctagcctgga atggacaaaa ccggagcgca tcgattggta gccgccgaaa ggcccaaccc
     1321 atcggtcaac cggaccgccc gcaagctgcg cttgcgggtt ccctccgcgc ttcgcgctcc
     1381 ggcggccggt tacctcaaac gtta
//
LOCUS       DAOQLC010000391         1399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_77419_length_1399_cov_12.951637, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000391 DAOQLC010000000
VERSION     DAOQLC010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77419_length_1399_cov_12.951637"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1399)
                     /locus_tag="PL000_22945"
     CDS             complement(<1..>1399)
                     /locus_tag="PL000_22945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNC91793.1"
                     /translation="VLVLLGLPILRAGFASLLRGQPNIHTLIMIGTFSAYALSVRNFM
                     LGHGGLYFDTATMLIFLVSIGRWLEMQAHKSSNKAVARLLEQIPNQAIVVSSDGDTTV
                     NVSDLKAGMRVRIRPGERFPVDGLIAIGEGDVDESLLTGEPKPVTHREGDKVQAGTVS
                     LDGSFEVIATAVGELTTAGQIGRLLHEALWARSPLERMADKLSAWMTPLALALATIAF
                     LIWYSFSGLETGLMVALSVLLIACPCALGLATPLTLWLSLERAAESGAILRSTGALER
                     LAKVERIFFDKTGTLSQLPMKVLGIKEESGKKKEFLEVVASVENESEHPLAKAIVEYA
                     KSQNIELIKPTSFKIIPAMGVIAQSSVVNGQLSIRIGSARLMSAEKLNMSAEISQQAE
                     AWKEAGHIVVYAGWDGQVMGILALGETIRAEAKETVGQLQSRGLELGVLTGDEASAGE
                     RWQKALGIPVSAALSP"
BASE COUNT          342 a          415 c          316 g          326 t
ORIGIN      
        1 gggctgagcg ccgcgctgac gggaatcccc aacgccttct gccatcgttc accagcgctg
       61 gcttcatcac ctgttagcac accgagttca agtccacgtg attgcaattg tccaaccgtc
      121 tcctttgcct cagcgcggat ggtttcaccg agcgcgagaa tacccatcac ctgaccatcc
      181 cagcccgcat acaccacgat atgtcccgcc tctttccacg cctctgcctg ttgactgatc
      241 tcagcagaca tattcaactt ttcagcagac atgaggcggg cggagccgat tcttattgac
      301 aattgaccat tgacgactga cgattgggca atcactccca tggcaggaat gattttgaat
      361 gaagttggtt taatcaattc aatattttgt gacttggcat attccacgat tgccttcgcc
      421 aatggatgct cggattcgtt ctcaaccgaa gcgactactt ccaaaaattc tttcttcttt
      481 ccactttctt ctttaattcc cagcactttc atcggcaact gacttagcgt gcctgtttta
      541 tcgaagaaga ttcgctccac tttggcaagg cgttccaatg cgcctgtgct gcgtagaatg
      601 gcgccagatt cagcggcgcg ttctaatgaa agccacaagg tcagcggagt tgccagcccc
      661 agcgcacatg gacaggcaat gagcaaaaca gagagggcaa ccatcaatcc cgtttccaat
      721 cctgaaaagg aataccagat caaaaaggca atcgttgcca gtgccaacgc cagcggagtc
      781 atccacgccg agagtttgtc tgccatgcgt tcgagcggag aacgcgccca caacgcttca
      841 tgcagtagcc gtccgatttg ccctgccgtt gttaattcac ccaccgccgt ggcgatgact
      901 tcaaagctgc catcgaggct cactgtgccc gcctgcactt tatcgccttc gcgatgagtc
      961 acaggcttgg gttcgcccgt taatagtgat tcatctacat ccccttcacc gatggctatc
     1021 agcccatcca cagggaagcg ttcaccaggg cgaattcgca cgcgcatgcc tgctttcaaa
     1081 tcagagacat tcacagttgt atcgccatca ctagaaacca caatcgcctg attgggaatt
     1141 tgctccaaca gtcgcgccac cgctttatta ctagatttat gcgcctgcat ttcaagccag
     1201 cgtccgattg aaaccagaaa gatgagcatg gtcgcggtat caaaatacaa gccgccatgc
     1261 cccagcataa aattccgcac cgataacgcg tatgctgaaa acgtcccgat catgatgagc
     1321 gtgtgaatgt tgggctgtcc gcgcagcagg cttgcaaacc ctgcgcgcaa aatcggcaga
     1381 cccagcaaaa ccaaaacag
//
LOCUS       DAOQLC010000392         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_77523_length_1398_cov_15.630678, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000392 DAOQLC010000000
VERSION     DAOQLC010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77523_length_1398_cov_15.630678"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..>1398
                     /locus_tag="PL000_22950"
     CDS             30..>1398
                     /locus_tag="PL000_22950"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNC91794.1"
                     /translation="MKGKLWPKIVAAMLGGLLIGAAHANIPSVPNELYEALKLDREKV
                     TPKELHEALVKRYKDPAQGSGRGTLAQYWEPIPYGIYLDPNTFYKSPTTNKEVASRKE
                     CVECHTDESPVWVQAWKRSSHANLDKVRNLKPGDPTFYKKAKLEDIEKNLRSMGRLAE
                     GENLKEVGCIDCHVDIGAKKKADHTKDIRMPTADVCGTCHLQEFAERESERDTMIWPH
                     DQWPDGRPSHALDYKANVETTVWAAMPQREVAEGCSMCHTNQNKCDSCHTRHEFSAAE
                     SRRPEACATCHSGVDHNNWEAYSMSKHGKIVGMLGNQWNWEAPLKDAYAVGGQSAPTC
                     AGCHMEYEGEYSHNMVRKIRWANYPFVPGIAENIKSEWSEKRLDSWVVTCTQCHSERF
                     ARSYLDLMDKGTLEGLAKYQEANAIVHQLYKEGLLTGQKTNRPAPPAPEKEGYAYFAQ
                     LFWSKG"
BASE COUNT          441 a          359 c          370 g          228 t
ORIGIN      
        1 aagaaaatgg aaaaccaaag gagagagaga tgaaaggcaa gctatggccg aagatagtag
       61 cggcaatgtt aggtggtctg ctgataggag cggcacacgc gaacataccg agcgtaccga
      121 atgaattata tgaagcattg aagctggatc gcgagaaagt aacgccgaag gaactgcacg
      181 aagcgctggt taagcgctac aaggatcctg cacaaggatc gggacgcggc accctggcgc
      241 aatactggga gccgataccg tacggtatat atttggatcc caatacattt tacaaatcac
      301 cgaccacgaa taaagaagtt gcaagccgta aagaatgcgt ggaatgccac accgacgaat
      361 caccggtatg ggtacaagca tggaagagaa gcagccatgc gaacctggac aaagttcgca
      421 acctgaaacc gggcgaccct actttttaca agaaagctaa gctggaagac atcgaaaaga
      481 acttacgctc gatgggcaga ctggctgaag gcgagaacct gaaagaagtc ggctgtatcg
      541 actgccacgt agacattggc gcgaagaaaa aagccgatca caccaaagac atcagaatgc
      601 cgacagcgga cgtttgcggc acctgtcacc tgcaagaatt tgcagaacgt gaatcggaac
      661 gcgacacgat gatctggccg cacgaccaat ggccggatgg acgcccatcg cacgcactgg
      721 actacaaagc caacgtagaa accaccgtat gggcagcgat gccgcaacgc gaagtagccg
      781 aaggctgctc gatgtgccac accaaccaaa acaaatgtga ctcatgccac acccgtcacg
      841 aattctcagc agcggaatcc cgcagaccgg aagcttgcgc aacctgtcac agcggcgtag
      901 accacaacaa ctgggaagcg tactccatgt ccaaacacgg caagatcgtt ggcatgctgg
      961 gtaaccaatg gaactgggaa gcaccgttaa aagacgccta tgcagtaggc ggacaaagcg
     1021 cgccaacctg tgccggctgc cacatggaat acgaaggcga atacagccac aacatggtta
     1081 gaaagattcg ctgggcgaac tacccgtttg ttcctggaat tgcagaaaac atcaagagcg
     1141 aatggtcgga aaaacgtctg gactcatggg ttgttacatg tacccaatgc cactcggaac
     1201 gttttgcccg ctcctatctg gacctgatgg acaaaggcac actggaagga ctggctaaat
     1261 accaagaagc caatgccatc gtacaccaac tgtacaagga aggcctgttg acaggacaaa
     1321 aaaccaaccg tcctgcaccg cctgcaccgg aaaaagaagg ctatgcctac tttgcccaac
     1381 tgttctggtc gaaaggca
//
LOCUS       DAOQLC010000393         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_79472_length_1380_cov_51.207547, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000393 DAOQLC010000000
VERSION     DAOQLC010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79472_length_1380_cov_51.207547"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..53
                     /locus_tag="PL000_22955"
     rRNA            <1..53
                     /locus_tag="PL000_22955"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            126..202
                     /locus_tag="PL000_22960"
     tRNA            126..202
                     /locus_tag="PL000_22960"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:160..162,aa:Ile,seq:gat)
     gene            209..284
                     /locus_tag="PL000_22965"
     tRNA            209..284
                     /locus_tag="PL000_22965"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Ala,seq:tgc)
     gene            580..>1380
                     /locus_tag="PL000_22970"
     rRNA            580..>1380
                     /locus_tag="PL000_22970"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          375 a          311 c          413 g          281 t
ORIGIN      
        1 tgaagtcgta acaaggtagc cgtaggggaa cctgcggctg gatcacctcc tttatagtcg
       61 tgtgctgtca agcgtttaca atttattggt tattgcaatg aatactgctt aagtaataag
      121 aaagtgggtc tatagctcag ttggttagag cacacgcttg ataagcgtgg ggtcggtggt
      181 tcgagtccac ctagacccac caaaagtagg gggtgtagct cagctgggag agcacctgct
      241 ttgcaagcag ggggtcatcg gttcgatccc gttcacctcc accaatcgcc gaaccaccac
      301 acgaagcggc aagcgcactt ttgcattcac cgaagcgcgc ttgccggttc gatcggaaga
      361 tcggctgtcg ttctttaaca attcatagag tcgaaatcag cgttgccggc ggaaagccag
      421 cgggcgtggc gcgagccgcg gccgtcccgg taccgtgccg ccggcaacac gattgcgtca
      481 ccaaacttca cttcatgcaa gtgaagaacg gcacaacgca aatactcatc agtcattgac
      541 cgacgccgca cctagcgaat gcgggcgtca gagttatagg gtcaagtgac taagtgcatg
      601 tggtggatgc cttggcgatt acaggcgacg aaggacgtga tagcctgcga taagcttcgg
      661 ggagctggca aattagcttt gatccggaga tttccgaatg gggaaaccca cccgcaaggg
      721 tatcgcatac tgaatacata ggtatgtgag gcgaaccggg tgaactgaaa catctcagta
      781 gctcgaggaa tagacatcaa ccgagattcc gaaagtagtg gcgagcgaaa tcggaggagc
      841 ctgcacgttt tagcagtcga catatcagaa cggaatggaa agtccggcca cagcgggtga
      901 tagccccgta tgaaaaatga cgattgtgga actaggcgtg cgacaagtag ggcgggacac
      961 gtgaaatcct gtctgaagat ggggggacca tcctccaagg ctaaatactc gtgatcgacc
     1021 gatagtgaac cagtaccgtg agggaaaggc gaaaagaacc ccgggagggg agtgaaatag
     1081 atcctgaaac cgcatgcata caaacagtgg gagcccgcaa gggtgactgc gtaccttttg
     1141 tataatgggt cagcgactta cattcagtag caagcttaac cgtgtagggg aagcgtagcg
     1201 aaagcgagtc ttaatagggc gttttagttg ctgggtgtag acccgaaacc agatgatcta
     1261 ctcatggcca ggatgaagcg agggtaacac ctcgtggagg tccgaaccca ctaatgttga
     1321 aaaattaggg gatgagctgt gggtaggggt gaaaggctaa acaaatctgg aaatagctgg
//
LOCUS       DAOQLC010000394         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_79955_length_1376_cov_17.277820, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000394 DAOQLC010000000
VERSION     DAOQLC010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79955_length_1376_cov_17.277820"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..>1376
                     /locus_tag="PL000_22975"
     CDS             254..>1376
                     /locus_tag="PL000_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91795.1"
                     /translation="MQSLKIFSTKTLQLIAPTNGRAVSKTLHVDALIQAIKTDFGKLT
                     DHRAKNAKVALDDALISAFAVFHLKDQSLLAFDERRRREPENLRTVYGVADIPCDSQM
                     RAILDEVDPAFLRPAFRTVFKRLQRGKKLESMTLLGGHYLLSGDGTGFYSSTKVASPY
                     CLKKTRRNGVELYYQQMYAAALVHPDCREVITFFPEMITRQDGSDKNDCERNAARRFF
                     EALRREHPHLKLIVTEDALSSNAPHIEDLQRLDLRFILGVKPGDHQFLFSWVDDAIVQ
                     GKVTELQRVDANDPGKIHFYRFINQVPLNQSRQDLLVNFLEYWQVDKNNKATRFSWVT
                     DLTITPDNAWEIMRAGRARWKIENETFNTLKNQGYNLEHN"
BASE COUNT          349 a          344 c          347 g          336 t
ORIGIN      
        1 cagcaattcc cgatgttccc tcaagccctg gttttatggg atacaaaggt atccggtttc
       61 gtaaactttt agagaacgag cttggttgca aaacaagttt tttgcggagt gcctggcaac
      121 cagctttgat gcaatgaacg cattaaaaat aaacgataaa ttttcaaggg tgacgctttt
      181 tttcttgaca acgcaatgag ggtaaaaatc gattttttca tggaattaca attaattaca
      241 tggagaatcg attatgcagt cactgaaaat tttctcgacc aaaactttac aactgattgc
      301 cccgacaaac ggaagagccg tgagcaaaac actccacgtg gatgcgttga tccaagccat
      361 caagacagat tttggcaaac tcaccgatca tcgggccaaa aacgccaagg ttgccctcga
      421 cgacgcgctg atatccgcct ttgccgtgtt tcacctgaaa gatcagtcac tgctggcctt
      481 tgatgagcga cgtcgtcggg aacctgaaaa tttacgcacg gtctatggag tggccgacat
      541 tccctgcgac agccagatgc gcgccatcct ggatgaggtt gatccagcct ttctccgccc
      601 ggcatttcgt actgttttca agcgtttgca gcgcggcaag aagctggaat cgatgacgct
      661 gctgggcggc cactatctgc tcagtggtga cggcacggga ttttactctt caaccaaggt
      721 ggcctcaccc tattgcctga agaaaacgcg tcgcaatgga gtggaattgt attaccagca
      781 gatgtatgcc gctgccctgg tgcatccgga ttgtcgcgag gtgatcacct tctttcctga
      841 aatgattacc cgtcaggacg gttcggataa aaacgattgc gagcggaatg cggcccgccg
      901 tttctttgag gcactgcggc gtgaacaccc gcatctcaag ctgatcgtca ccgaggatgc
      961 gctgagcagc aatgcgcctc atatcgagga tctgcaacgg ttggacctcc gctttatcct
     1021 tggcgtcaaa cctggggatc atcagtttct gttttcatgg gttgacgatg ccattgtcca
     1081 aggcaaggtc accgagctgc agcgggtgga tgccaacgat ccgggaaaga tacattttta
     1141 ccgttttatc aaccaggtac cgctcaacca atctcgccag gatctcctgg tcaatttcct
     1201 ggaatactgg caggtggata agaataataa ggctacccgg ttcagttggg tcacagatct
     1261 gacgatcacc ccggacaacg cctgggagat tatgcgggct ggccgtgctc ggtggaagat
     1321 cgaaaacgaa accttcaaca ccttgaaaaa tcaggggtac aacctggagc ataatt
//
LOCUS       DAOQLC010000395         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_80181_length_1374_cov_14.780895, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000395 DAOQLC010000000
VERSION     DAOQLC010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80181_length_1374_cov_14.780895"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..788)
                     /locus_tag="PL000_22980"
     CDS             complement(33..788)
                     /locus_tag="PL000_22980"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91796.1"
                     /translation="MELKPHRVEYWLRAKPGDPDEFEAQVRQVCDIYEQAPALHQAGI
                     HVISSDEKTGIQALERAAPTLGVKPGRVERQEYEYVRHGTQCLIANFEVATGKVIAPT
                     ISLTRTEEDFAAHLAQTILTDAEAGWIFVTDNLNTHVSETVVRLVARLCGIGADLGVK
                     GKTGILESMTTRAEFLSDVTHRIRFVYTPKHTSWLNQVEIWFSILVRRLLKRASFKSV
                     EELKARILAFIEYFNQTMAKPFNWTYKGRPLTI"
BASE COUNT          270 a          436 c          287 g          381 t
ORIGIN      
        1 ctagtggttc ttggcagaaa taatttaaca attcaaatcg tcagtggacg acctttgtaa
       61 gtccagttga acggtttggc catcgtttga ttaaaatact caatgaatgc cagaatccga
      121 gctttcaact cctcaaccga tttgaaactg gctcgtttga gcaaccgccg gaccaggata
      181 ctgaaccaaa tctcgacttg attgagccaa ctggtgtgct tcggggtata cacaaaccga
      241 attcgatggg tcacatctga gagaaactcg gctcgggttg tcatcgattc caagattcca
      301 gttttgcctt tgactcccaa atccgcccca attccgcaga gtctggccac caaccgaacc
      361 acggtttctg agacatgggt attgagattg tcggtcacaa agatccaccc cgcctcggca
      421 tcggtcagga tcgtttgggc taggtgggcg gcaaaatctt cttccgtgcg ggtcaaagaa
      481 atagtcggtg cgatcacttt cccggtggcc acttcaaaat tggcgatcaa acattgggtc
      541 ccatgacgca catattcata ttcttgccgc tcgacccttc ccggtttcac cccgagcgtc
      601 ggcgcggccc gttccaatgc ctgaatcccc gttttctcgt cgctgctgat gacgtgaatc
      661 ccggcctggt gcaacgccgg cgcttgctca tagatgtcac acacctgtct cacctgcgct
      721 tcaaattcgt ctggatcgcc cggcttggcc ctcagccagt attcgacccg atgcggtttg
      781 agctccacct gattttaaaa aacgcccgac ttgcctcacg ctgatactct ccacgatccc
      841 tcgtttcacc acttcgtcgg ctaattccct tggcgtccaa tgactgattg gtcgctcgct
      901 tgcctcctcc ggtttttcac accccactgc cacaatcgct accacttgct ccgcccgaaa
      961 ctttaccggc gctccgctcc ggggctcgtc gctgagcacc ttcaccacta ccgcctccaa
     1021 tttcttatca tcctccactt cctcgacccc ttccatccgt tcgctctctg ctatccaatg
     1081 cctcctccag tacccaattc gttcccggct caaccccacc tccggttcaa tcgccgtatt
     1141 cgtttttcct tccccaaacc ccaggatcac tttcgctcgc ttcaccagtc cttgtggact
     1201 gcttgggcgt cggatgatcc gttccagaat tgctcgttgt ctctgactta accggattgg
     1261 tttgactcgt gcatctgcca tacctttgac tcccttttct tctcattttt cgcttttgga
     1321 gtcgctattt tgctttctca ttggtgttaa gtcaattcag ccgaagacca ctag
//
LOCUS       DAOQLC010000396         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_80393_length_1372_cov_24.585421, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000396 DAOQLC010000000
VERSION     DAOQLC010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80393_length_1372_cov_24.585421"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PL000_22985"
     CDS             <1..1023
                     /locus_tag="PL000_22985"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNC91797.1"
                     /translation="EARTTHANGFKPKTIKTRVGEIPLAVPQTRDGKFYPNCLEKGQR
                     SEKALTLAIAEMYLQGVSTRKVMSITQELCGFEVSSASVSKATSQLDELFSSWRERPL
                     DRFRYVYLDARYENVREGGVVIDQAVLVAVGVSESGKREVLGVSVELSEAEIHWRKFL
                     HSLQHRGLHGVQMFISDDHAGLKAARKAVFPAVPWQRCQYHLQQNAQKHVPKQEMKLQ
                     VAADIRNIFNAPDGKEAERLLSKTVASYEKTAPRLSNWMAENIPEGLTVFKLPAKHRI
                     RTRTTNGVERLNKEIKRRTRVVSIFPNSASCLRLVTAVVMEISEDWQTDKCYLSFANT
                     PSQPVE"
BASE COUNT          376 a          331 c          370 g          295 t
ORIGIN      
        1 gaggcaagaa ccacacatgc caacgggttt aagccaaaga cgattaagac aagagtaggt
       61 gagataccgc tagcggtccc gcagacaagg gacgggaaat tctatcctaa ctgcctagag
      121 aagggtcagc gctccgagaa agcacttaca ttggctatag cggagatgta tctacagggc
      181 gtatcaacgc gcaaagtgat gtcgataacc caggagctgt gcggctttga agtcagttcg
      241 gcttctgtca gcaaggcgac atcccagctt gatgagctat tctcctcgtg gagagagcgg
      301 cccctcgatc gcttccgcta tgtataccta gatgcacgct acgagaacgt gagagaggga
      361 ggtgttgtca tagatcaggc agttcttgtt gcagtcgggg tatccgagag cgggaagcgc
      421 gaggtcttgg gagtgtcggt tgagctctct gaagcggaga ttcattggag aaagttcttg
      481 cattctctcc agcacagagg cttacacgga gtccaaatgt tcatcagtga tgatcatgcg
      541 ggactaaaag cggctcgcaa agcagtattt ccggctgttc catggcagag atgccagtac
      601 catctccagc agaatgcgca aaagcacgtg ccaaagcagg aaatgaagct tcaggtcgca
      661 gcggacatac gtaacatatt caatgcgccg gatgggaagg aagcggaaag gcttttgagc
      721 aagaccgtag caagctatga gaagacagcc ccacgcctat caaattggat ggcggaaaat
      781 ataccggaag gtcttacggt cttcaaattg ccagccaaac atcgaattcg gactcgaact
      841 actaatggtg tcgagcgcct gaacaaggag attaagcggc gcacacgggt tgtatcgatc
      901 ttcccaaact cggcctcatg cctgcgacta gtaactgcgg ttgtgatgga gatctccgag
      961 gactggcaga ctgacaaatg ctacctaagt ttcgctaaca cgccgtccca accggtagaa
     1021 tagagcgaca catctatttg gcagtggcag ctcatgcgta atagagcgcc ctgagcgaac
     1081 gagatagctc acctccccaa aaggtgggct gtctcgttcg caatcacata caaacgtcgg
     1141 ggacaatgcc gtggcgccag gacctggcgc ggctgtttca tccaatctat caagtacagg
     1201 atcagcggat gatactactg gcagtaccgc agagtcctgc tttgtactcg aaaatcttca
     1261 cctgttaatt gtcttttttg tggtatattc aaaatcaggt cttcgagcta tatgacacca
     1321 gaaagattaa accgctccgg gactgaattt acagaacaaa aaatgctccg cc
//
LOCUS       DAOQLC010000397         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_80394_length_1372_cov_19.454062, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000397 DAOQLC010000000
VERSION     DAOQLC010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80394_length_1372_cov_19.454062"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1135)
                     /locus_tag="PL000_22990"
     CDS             complement(182..1135)
                     /locus_tag="PL000_22990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC91798.1"
                     /translation="MNTQKTLAKAIGAAAIALSGTASAALFMPPVNQPLFIKFTNKEQ
                     ICTDGSNCITPPTITPGYNLPSGLSTEQNWGIIDITQVFLGDVAIDPILWPKTGFPIF
                     NEGQNGGQQITGIFYGIQAYPPSAANPIRSTSGYLDLWFDEPGTGGGGTLADFANATP
                     GMRTSANEFGSFTDGIFLGRIAYAPGIDTLGDPNVNIIGSIDPNQVGQGLTGFADSYG
                     DVADVNGDTIIDSMDGAWAALLNGDYFPVLVDHGAGLVQEKRDFRFKNSYNLLTQWSD
                     SGITGAISDDPARVFTVPEPGSLALMGLGLVGAGLISRRRK"
BASE COUNT          283 a          350 c          425 g          314 t
ORIGIN      
        1 ctcgctgact ttcatgacca agtatgccat ggcctttctc gcactgccca accggaaaat
       61 gggaaattgg cggaaaattg aggaaaccgt ggtctgtccc cgttttccgt ccccgttttc
      121 cgggcaacaa aaaaaggcgg ctccaactgg agccgccttg atcgcagtcg tcgcgtggcg
      181 attattttct gcgacggctg atcaggccag cgccgaccag acccaagccc atcagcgcca
      241 gcgagccagg ctcgggaacc gtaaagaccc gggcggggtc atcactgatt gcgccggtga
      301 tgccgctatc gctccactgc gtcagcaggt tgtagctgtt cttgaagcgg aagtcacgtt
      361 tctcttggac cagtccagcg ccgtgatcaa ccagtacggg gaagtagtcg ccattcaaca
      421 gtgcggccca tgcgccatcc atcgagtcga ttatggtgtc gccgttgaca tccgcgacat
      481 ccccgtagct gtcggcaaag ccagtgaggc cctgtccaac ttggtttgga tcgatgctgc
      541 caatgatgtt gacgttgggg tcgccaagag tatcgatgcc gggggcatag gcgatgcggc
      601 cgaggaagat gccatcggtg aagctaccga actcattggc ggaggtgcgc atgccgggcg
      661 ttgcattagc gaaatcagca agggtgccgc cgccgccggt gcccggctca tcgaaccaga
      721 ggtccagata gccggaggtg ctgcggatcg ggttggctgc agaaggtgga tatgcctgaa
      781 tgccgtagaa gataccggtg atctgctgac caccgttctg cccttcgttg aagatgggga
      841 agccagtctt gggccaaaga attggatcaa tagcaacgtc gcccaagaaa acctgggtga
      901 tgtcgatgat gccccagttc tgctcggtgc tgagtccgct gggcaagttg tacccagggg
      961 ttatggtcgg tggcgtgatg cagttggagc catccgtaca gatctgctcc ttgttggtga
     1021 atttgatgaa cagtggctgg ttgacaggcg gcataaaaag cgctgccgat gcagtacctg
     1081 agagagcgat ggcggctgcg ccgatggcct tggccagggt cttttgagtg ttcacggtgt
     1141 gatctccatg ttgggtcaat cgttgctggt gcgcagtcgg ctgtcgggtg ttgctgggtt
     1201 ggcgccacgt tcaccgctgt tgcaatgata taaggcacgg ggcgtgccat tttttatttc
     1261 ttatataaaa tcaacaagtt gatcaataaa acgtgagctt gcgctggacg atttcggcaa
     1321 aagtgtaaaa tttgttgaca cttttgcggc tgggtcgatg aacgcccatg tc
//
LOCUS       DAOQLC010000398         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_80709_length_1369_cov_28.779300, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000398 DAOQLC010000000
VERSION     DAOQLC010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80709_length_1369_cov_28.779300"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..>1369
                     /locus_tag="PL000_22995"
     CDS             75..>1369
                     /locus_tag="PL000_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91799.1"
                     /translation="MTQRNTGSNSGWQWLPLLMLGAVLGLTLWLYWPGLTGPFLLDDE
                     YNIAPLMLGNYSPSAIWYALTHNESGLFGRMLSTASLLLTGWLHGPGSWGFKYHNLLI
                     HLLTGLLLVGCCGRLLCWRLAQRDAWRLAVLAGLFWLLHPLQVSTVLYPVQRMAQLGV
                     LFTVAGLYVALLGLEQARDGRRGRAIGLLFFGYPLLLAMAVASKESGVLLLPLTLLAL
                     GFIWPTLRPQQGLVGLQRNFALLLVLLPLLLGLLYFALRWPRLLDYSGRLFTLPERIY
                     TQLHVLWFYLKLILLPRLGEMGLYHDDFATVSQPSLLTAVAGAGLVALLAVAWLARRR
                     WPVVSLGIGWFFVGHLLESTLLPLEMVFEHRNYLAAFGPLLLVAYGILAFSPSMRLFK
                     WGVVVTLLCSFALMTSARAKVWSDDGLLQEMSVQEHPDSA"
BASE COUNT          169 a          425 c          468 g          307 t
ORIGIN      
        1 ttgaccctca ggggacaaca gaggctggcc atcccggtgg ccagcctttt tttgtttggt
       61 cagcgcagca cgtcatgacg caacgcaaca caggttcgaa ttcgggttgg caatggctgc
      121 cgctgctgat gctgggcgcc gtgctggggc tgacgctgtg gctctactgg ccgggtctga
      181 ccgggccctt tctgctcgat gatgagtaca acatcgcccc gctgatgctg ggcaactaca
      241 gcccgtcggc gatctggtat gcgctgaccc acaacgagag cggcctcttt gggcggatgc
      301 tctccaccgc cagcctgctc ctcaccggct ggctgcatgg tccgggctcc tggggcttca
      361 agtatcacaa cctgctgatt catctgctga ccggtctgct gctggtcggc tgctgtggcc
      421 ggctgctctg ctggcggctg gcgcagcgcg atgcatggcg tcttgccgtg ctggccggtc
      481 tgttctggct gctgcatccg ttgcaggtga gcacggtgct ctacccggtg cagcgcatgg
      541 cgcaactggg cgtgctcttc acggtggccg ggctctacgt ggcgctgctg ggactggagc
      601 aggcccgcga tggccgccgt ggccgggcga tcgggctgtt gttcttcggc tatccgctgc
      661 tgctggcgat ggcggtggcc agcaaggaga gtggggtgct gctgctgcca ttgacgctgc
      721 tggcactcgg cttcatctgg ccaacgttgc gcccgcagca gggactggtc ggattgcaac
      781 ggaacttcgc gctgctgctg gtgctgctgc cgctgctgct gggcctgctc tactttgccc
      841 tgcgctggcc gcgcctgctc gactacagcg gccggctgtt cacactgccg gagcgcatct
      901 acacccaact gcatgtgctc tggttctatc tcaagctgat actgctgccg cgactgggcg
      961 agatggggct ctaccacgat gactttgcca ccgtcagcca gccatcgctg ctcaccgcag
     1021 tggctggggc cggtctggtt gcgctgctgg ccgtggcctg gttggcgaga aggcgctggc
     1081 cggtggtgtc gctcggcatc ggctggttct ttgtcggcca tctgctggag tcgaccctgc
     1141 tgccgctgga gatggtgttc gagcaccgca actatctggc ggccttcggc ccgctgctgc
     1201 tggtggccta tggcattctg gccttctcgc cgtcgatgcg cctcttcaaa tggggggtgg
     1261 tggtgacgct gctgtgcagc tttgccctga tgacctcggc ccgcgccaag gtgtggagcg
     1321 acgatggcct gctgcaggag atgtcggtgc aggagcatcc cgactcggc
//
LOCUS       DAOQLC010000399         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_82602_length_1353_cov_14.657165, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000399 DAOQLC010000000
VERSION     DAOQLC010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82602_length_1353_cov_14.657165"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PL000_23000"
     CDS             <1..538
                     /locus_tag="PL000_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNC91800.1"
                     /translation="DVVYADDFGPLAFISKARSGILRDFGTVQQPIASWFLLQGLDTL
                     SLRMERHVQNAQRVAEFLESHPNVKWVSYAGLASHPHYERAKKYLPLGTGAVMGFGVK
                     GGREAGAKFIDNLQLFSHVANIGDTRSLAIHPASTTHSQLSDEEQTAAGVSPDFIRLS
                     IGLEDIEDILWDLDQALG"
     gene            complement(558..1154)
                     /locus_tag="PL000_23005"
     CDS             complement(558..1154)
                     /locus_tag="PL000_23005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC91801.1"
                     /translation="MGRKDLTEQRHEDILDAFERCLIKYGLEESTLERVAQEAGKSRN
                     IIRHYIGNREDLIAAFVERILSRIKQISEEILADTPKQKLIPNVLDFLFEERKADAPP
                     DLGERMLGGMWHIREQSPKVQAALLNFYQEFEKILTDGLSQLYPNVSAKKCQEVAYSI
                     ICLAETNWVLGSVGVDVSHTRMARRSAEYLLSVLDNPR"
BASE COUNT          329 a          365 c          308 g          351 t
ORIGIN      
        1 cgacgttgtc tacgccgatg actttggtcc gctggcgttc atctcaaaag cgcgttcggg
       61 catcctgcgt gatttcggca ccgtgcaaca acccatcgct tcgtggttcc tcctgcaagg
      121 actcgatacc ctctccctgc gcatggaacg tcatgtgcag aacgcgcaac gtgttgctga
      181 atttcttgaa agccacccca atgtcaaatg ggtctcgtac gcgggtctcg ccagtcaccc
      241 ccattatgaa cgcgcaaaaa aataccttcc gcttggaaca ggcgcggtga tgggattcgg
      301 cgtaaagggc ggcagagagg cgggcgcgaa attcatcgac aacctgcaac tcttcagcca
      361 cgtcgccaat atcggcgata cacgctcatt ggcgatccat cccgccagca ccacccacag
      421 ccaactctcc gacgaagagc aaaccgccgc aggtgtcagc cctgatttca ttcgtttatc
      481 gattggcttg gaagatatcg aagatattct ctgggatctg gatcaggcat tggggtaatc
      541 atctgccttt agaaatgtta tctgggatta tctaaaacac tcaacaaata ctctgccgaa
      601 cggcgcgcca tgcgcgtatg gcttacgtct acacccactg aacccaatac ccagttcgtc
      661 tctgctaggc agatgatgga ataggccact tcctgacatt tttttgctga cacattcggg
      721 taaagctgtg aaagtccatc ggtcaaaatc ttttcaaact cctgatagaa gttcagcaat
      781 gccgcctgca ctttagggct ttgctcgcgg atatgccaca ttccgcccaa catgcgctct
      841 ccgagatcag gtggggcatc tgctttacgt tcttcaaaga gaaaatccaa tacattggga
      901 atcaatttct gtttgggggt atctgccaga atctcttctg agatctgttt aatacgggac
      961 agaatccgct ctacaaacgc agcgatcaaa tcttctcggt tgccgatata gtgacggatg
     1021 atgtttcggc ttttgcccgc ttcctgtgcc acgcgttcca gcgtgctttc ttccaatcca
     1081 tacttgatca ggcatcgctc gaatgcatca aggatgtctt cgtgtctttg ttcggttaag
     1141 tcttttcgtc ccatgccaac catccatttg ataaatcagg taggggagat tataccaatt
     1201 cgcgaaataa attgtcaaaa agacaatcta ttgacaaata cagcgtggtt gcctataata
     1261 aattgtcgtt gagacaattt attatttcag gagaagatta tgcccaaact gttgacctcc
     1321 accccacacg cggacggttt ccgcatgccc ggc
//
LOCUS       DAOQLC010000400         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_83403_length_1346_cov_18.386522, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000400 DAOQLC010000000
VERSION     DAOQLC010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83403_length_1346_cov_18.386522"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PL000_23010"
     CDS             complement(<1..1013)
                     /locus_tag="PL000_23010"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC91802.1"
                     /translation="MSFDPTYPHRLDLLPPQKNIANTDFGDLLIKARVELAELKGACG
                     QIPNPLLLMSPAIIRESVASSNIENINTTLADVLQWQLFPEAEQRQPDKEVLRYREAM
                     NWGFENAEKYALSSRLITGIQARLMPDGNGQYRREQNQIANLASRESLYTPPIANNIP
                     SLIGNWENYVNTLDESLDPLIRIIISHYQFEAIHPFRDGNGRTGRILMILQLIQYGLL
                     QFPVLYISGYINQHRSDYYRHLLAVTKEQNWHGFIEYMLEGFYTKAMETREDLKKITV
                     LFEQIKDQIKTENKKIYGLDLVETLFTYPVITPTKLASELNMHYTTTSRYLAQLAEMG
                     ILK"
BASE COUNT          352 a          277 c          328 g          389 t
ORIGIN      
        1 tctttcaaaa ttcccatttc ggctaattgc gccagatacc tgcttgttgt ggtgtagtgc
       61 atattcaact cgctggcgag ttttgtgggc gtaataacag ggtaggtgaa taaggtctca
      121 accaggtcaa gaccatagat ctttttattt tctgtcttga tctggtcttt tatttgctca
      181 aaaagaaccg ttattttttt taggtcttcg cgggtttcca tggcttttgt ataaaaacct
      241 tcgagcatat attcaatgaa tccatgccag ttttgttctt ttgtaacagc caatagatga
      301 cgatagtaat ctgaacggtg ttgattaatg tatccgctga tgtaaagcac gggaaattga
      361 agcagcccat attgaatgag ttgcagaatc atcaagatgc gccccgtcct gccgttgcca
      421 tcgcggaatg gatgaatggc ttcaaattgg tagtgagaaa taatgatgcg aatcagcggg
      481 tcgagagatt cgtctagtgt attgacgtag ttttcccaat taccaatcag gctgggaatg
      541 ttgtttgcaa tcggcggggt atataaactc tcacggctgg caaggttagc gatttggttt
      601 tgttcgcggc ggtattgacc attgccatcg ggcattaatc gcgcctgaat ccctgtgatg
      661 agccgtgaag acaaggcata tttttccgca ttttcaaaac cccaattcat ggcttcacga
      721 taacgcaata cttccttgtc aggctggcgc tgctccgctt cgggaaataa ttgccattga
      781 agcacatccg ccagagttgt gttgatgttt tcgatattgg aactggcaac cgattcacgg
      841 ataatggcag gcgacatgag caaaagcggg tttggaattt gtccacatgc acctttgagc
      901 tccgccaatt ctacgcgtgc cttgataagc aaatcaccaa aatcggtatt tgcaatgttc
      961 ttttggggcg ggagcaggtc tagcctgtgg ggatatgttg gatcgaatga catattgaaa
     1021 tcaatatatc agttttttag cttttttgca atatataatc aggtcaactc caaacgggga
     1081 agggaccttt ccccccgccc ttctctaaag ggaagggggc tgagttcgtg atcggcagag
     1141 ggaatgtcca tgcgaggagg gttttagctt ctccccttta ggggagacgg gagagaggtt
     1201 ttatcgtaga atttctccat gccaattaaa aatattgtta ccgaacaacg agtaacaaaa
     1261 gcaaaacttg aacgtgccaa agaactgcgt cgtgaaatga cgcccgcaga aaaacttctc
     1321 tggcaggaaa tccgcgccaa taaact
//
LOCUS       DAOQLC010000401         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_83406_length_1346_cov_14.298993, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000401 DAOQLC010000000
VERSION     DAOQLC010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83406_length_1346_cov_14.298993"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1256)
                     /locus_tag="PL000_23015"
     CDS             complement(132..1256)
                     /locus_tag="PL000_23015"
                     /inference="COORDINATES: protein motif:HMM:NF025448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HNC91803.1"
                     /translation="MTKNKRKQSKRSPRSALELLMEQKAKSFLVAEKGYWEAISSLRR
                     QRQESANSLDLPFEENLPENISSTVWGLKDGVAFVRHKTVGTTPRVDFRLLPITLEEW
                     GNDGLVVPLETGEISLARAGLIRGTGNISMINCVFNDIHLPFVEFSHLRYGDSTNTPS
                     VERAVLDFQLALLGLQTRPSSDANPQSVTGAHTIVVLKQLADNFASLIAAGTKEEELQ
                     VFLKANPFILHPSAECIPKKKLGEDFVTDFVLVATTTQGPTYILVELERASHQILTKD
                     LVLSGPVNHAIKQTRDWDVWLEKNKAYIQNKLPGFETPSYLVVIGRGDALTEEERAYL
                     RSYNREWKNTSLLTYDDVLARFRSTISNLETIASGAAGPQ"
BASE COUNT          297 a          338 c          359 g          352 t
ORIGIN      
        1 gaagaaagat ggcgctgggg tgactacacc agcgcgcagc gctttagttc aacgtagaag
       61 tcaccggcgc tgcacggctt tatcgcgcag cgtccgtgtg gactgccggg ttgggcgatt
      121 tgcagaccta gtcactgcgg gcctgccgcg ccgctagcga tggtttcgag attgctaatg
      181 gtgcttctga accgtgccaa aacatcatcg tatgtgagga gtgacgtgtt cttccattca
      241 cggttgtatg accgaagata tgctctctct tcttcggtca gcgcatctcc ccttcctatt
      301 accaccagat aggagggagt ttcaaatccg ggaagcttgt tttgaatgta cgccttgttc
      361 ttctccagcc aaacatccca gtctcgtgtc tgcttaatgg catggtttac cggcccggac
      421 agtacaaggt ctttggtcag aatttggtgt gaggctcgtt cgagttcgac aagtatgtag
      481 gtgggacctt gcgtggttgt cgcaaccagt acgaagtcag tgacgaagtc ttcgccaagc
      541 ttcttctttg gtatacattc cgctgacgga tgcagtatga acggattggc tttgaggaat
      601 acctgtagtt cttcttcttt cgtcccggca gctataagcg atgcgaaatt gtcggcgagt
      661 tgcttcaaga caactattgt atgagcgcct gttacggact gtgggtttgc gtcggaactg
      721 gggcgcgttt gtaagcccag taacgccaat tgaaaatcaa gaactgcacg ttcaaccgaa
      781 ggcgtgtttg tcgagtcgcc atatcgcaag tgcgaaaact cgacaaacgg caaatgaatg
      841 tcattgaaca cgcagttgat cattgaaatg ttgccggttc ctcgaattag gccagctcgc
      901 gcgagtgaaa tttctccggt ttcgagggga acgaccaagc catcattccc ccattcctcc
      961 aacgtgatcg ggagtaatcg aaagtctacc ctgggtgttg tgccgaccgt tttgtggcga
     1021 acaaatgcga ctccatcctt aagtccccac acagtgcttg agatgttttc agggagattt
     1081 tcttcgaacg gaagatccag agagtttgcg ctctcctgac gctgacggcg caaagacgaa
     1141 attgcttccc agtaaccctt ttcggcgacc agaaaactct tggccttctg ctccataagc
     1201 agttccaacg cagaacgcgg tgatcgcttt gactgctttc gtttgttctt tgtcataggt
     1261 gatcgcctag cgtgaatggt gatttcaaag cgcccaacga atagcgttta tctgccgcgc
     1321 cgaaagcgcc gggtccagcc gcaagc
//
LOCUS       DAOQLC010000402         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_84150_length_1340_cov_14.347082, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000402 DAOQLC010000000
VERSION     DAOQLC010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84150_length_1340_cov_14.347082"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PL000_23020"
     CDS             complement(<1..1002)
                     /locus_tag="PL000_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNC91804.1"
                     /translation="MEYRKLGRSDLLVSPLCLGTLNFGGPTNEQDSLAIMHEALEGGI
                     NFFDTANLYYNGDGERIIGKFLKEQRVRDQVVLATKVAVHMGDLPAEAGAARAHILKA
                     CEDSLQRLQTDHIDLYQLHRPPSDHSQEEVLRAFDDLIRSGKVRFIGCSTHPAWMVME
                     ALSISEKQGFNRYVSEQPPYNLLDRRIENELIPLCQKYDLAIMPYSPLAVGVLAGHYT
                     QTGKYPEGSRAQNWDKSLSESRITQRGIEVGNAVLKLAQERGLTASQLSLLWTKDQPG
                     ITAPIIGPRTSAHLKDALAILNKTLDEADRPLFDALVHPGNAVADFHNNNAWMKTRVM
                     "
     gene            1109..>1340
                     /locus_tag="PL000_23025"
     CDS             1109..>1340
                     /locus_tag="PL000_23025"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC91805.1"
                     /translation="MTSNQYDAIIIGGGHNGLVAGAYLARAGKKVVVLERRPIVGGAA
                     VTEEIFPGYKFTEFSYVVSLLRPEIIRDLELPK"
BASE COUNT          329 a          336 c          331 g          344 t
ORIGIN      
        1 catcacccgg gttttcatcc atgcattgtt attgtgaaaa tctgcgactg cattcccggg
       61 atgaacaagc gcatcaaaca atggacgatc ggcttcatcc agcgttttat tcagaatcgc
      121 aagcgcatct ttcaagtgtg ccgatgtgcg tggcccaatg atgggagcag tgatgccagg
      181 ttggtcttta gtccacaaca aggaaagttg cgaagcggtc aaaccgcgtt cttgagccag
      241 tttaagtaca gcattgccta cctcgatccc acgttgtgtg atacgggact cgctcaggct
      301 cttatcccag ttttgagcac gtgagccttc agggtatttc cccgtctggg tatagtgccc
      361 cgctaatacg ccgactgcca acggtgaata tggcatgatg gcaaggtcat atttttggca
      421 taacggaatc agttcgtttt caatgcggcg atcgagcagg ttgtatggag gttgttcgct
      481 gacatatcga ttgaatcctt gtttctcact gatggataaa gcctccatca ccatccaggc
      541 aggatgtgtg gaacaaccaa tgaagcgaac cttgcctgaa cggatcaaat catcaaacgc
      601 ccgtaacacc tcctcttgcg agtggtccga cggtggacga tgaagttgat acaagtcaat
      661 atggtctgtc tgcaagcgtt ggagtgaatc ttcgcaagcc ttgaggatgt gcgcacgggc
      721 tgccccggct tcagcaggca ggtctcccat atgcaccgct acttttgtag ccagcacaac
      781 ttggtcacgc acacgttgtt cttttaaaaa cttcccgata atgcgctccc catcgccgtt
      841 gtagtacagg ttggctgtat cgaaaaaatt aataccgcct tccagcgcct catgcatgat
      901 cgcaagcgaa tcctgttcgt tggttggacc tccaaagttc agagttccca aacacaacgg
      961 cgaaaccagc aagtcagaac gtccgagttt tcggtattcc atatgcacat ccttttgaca
     1021 agaagatgtt acagaatacc attttttgtt tcgaatagta tagggttaat actcacgact
     1081 taaattattt tgagaaaagg aaggcttgat gacatccaat caatatgacg caatcattat
     1141 aggcggcggc cacaatggac ttgtggctgg cgcatacctt gcccgtgctg gcaagaaagt
     1201 ggtcgtgctc gaacgtcgac ccattgtcgg cggtgcggca gtgaccgaag agatcttccc
     1261 cggctacaaa ttcaccgagt tctcgtacgt ggtgagtctc ttgcgcccgg agatcatccg
     1321 tgacttggaa ctgcccaagc
//
LOCUS       DAOQLC010000403         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_86046_length_1325_cov_17.503937, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000403 DAOQLC010000000
VERSION     DAOQLC010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86046_length_1325_cov_17.503937"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PL000_23030"
     CDS             complement(<1..526)
                     /locus_tag="PL000_23030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91806.1"
                     /translation="MQNQNLNHGDAPERASGRRETLRTKFLSAFVSPWFSRLSLWLAP
                     ILFLLVFFFQPLARIISLAFNSSAFTQKNFETILSVTGFTFYQAILSTILTFALGLPS
                     AVLFSRFDFHRKSLLRALTAVPFMLPTVVVASSFNALFGNHGLFSFFILHPSPFILIL
                     LAHVFYNTTIVIRIV"
     gene            complement(535..>1325)
                     /locus_tag="PL000_23035"
     CDS             complement(535..>1325)
                     /locus_tag="PL000_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC91807.1"
                     /translation="VDNTFLSRALEADIFEAYASPALSDISDEFKLDSSNRAQPVDYG
                     DVCINYDKAYFAENNLAVPQSFEELAKPEYKGLLVVENPATSSPGLAFLLATRAHFGD
                     GYLDYWKSLKENGAVVVDGWETAYYTNFSASSGKGPQSMVVSYASSPAAEVFFASPQP
                     SESPTASIIASGMCFRQVEFVGILKNTSNQPLAQKFVDFMLSQKFQEDMPLNMFVYPV
                     NKNAQLPEVFTSFAQVAESPATLSAAEIASNRDAWIEAWTAAMK"
BASE COUNT          358 a          268 c          411 g          288 t
ORIGIN      
        1 cgacgatgcg gatgacgatg gtggtgttgt agaaaacgtg ggcgagaaga ataaggatga
       61 aaggtgaagg atgaaggatg aagaaagaga acaaaccgtg attgccgaag agcgcattga
      121 aacttgatgc gacgacaacg gtgggaagca tgaaggggac agccgtgagt gcgcggagga
      181 gtgatttgcg gtgaaagtcg aagcgtgaga ataggacggc ggatggcagt ccgagggcga
      241 aagtaagaat ggtggagaga atcgcttgat aaaaagtgaa gccagtgacg gagagaatgg
      301 tttcaaaatt tttctgtgtg aaagcagaag aattaaacgc aagagagatg atccgcgcga
      361 ggggttggaa gaagaaaacg agcaggaaca ggattggcgc gagccaaagt gaaaggcggg
      421 aaaaccacgg agacacgaag gcactgagaa atttcgttct cagtgtctca cgtcgtcccg
      481 acgctctttc gggggcgtct ccgtggttta aattttgatt ttgcatttaa tggtttattt
      541 cattgccgct gtccacgctt cgatccacgc atcgcggttg gatgcgatct ctgcggcgga
      601 gagggtggcg ggactttctg caacctgggc aaagcttgtg aagacttcgg gcaattgggc
      661 gttcttattg actggataaa cgaacatgtt cagcggcata tcttcctgaa atttctggct
      721 caacataaag tccacaaatt tttgggcaag gggttgattg gacgtgttct tcaagattcc
      781 cacgaattca acctgacgga agcacatacc cgaggcaatg atggatgcgg tcggagattc
      841 gcttggctgg ggcgaagcga agaacacttc cgcggcgggg ctggatgcgt acgaaacgac
      901 cattgactgc ggtcccttgc cagaggatgc ggagaagttt gtgtagtagg cggtttccca
      961 tccgtccaca acgacagcgc cgttttcttt cagactcttc cagtaatcaa gatacccgtc
     1021 accgaagtga gcgcgggtgg caagcaaaaa ggcaagccca ggtgacgagg tggcaggatt
     1081 ctccacaacc agcaatccct tgtattcagg ctttgcgagt tcttcaaatg attgcggaac
     1141 agcgagattg ttttcggcga aataagcttt atcgtaattg atgcacacat cgccatagtc
     1201 cacaggctgg gcgcggttgg atgaatcaag tttgaattcg tcggaaatat cgctcaacgc
     1261 aggcgaggcg taggcttcga aaatgtccgc ttcaagagcg cgggagagga aggtattgtc
     1321 cacgc
//
LOCUS       DAOQLC010000404         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_87075_length_1317_cov_17.096672, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000404 DAOQLC010000000
VERSION     DAOQLC010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87075_length_1317_cov_17.096672"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="PL000_23040"
     CDS             <1..780
                     /locus_tag="PL000_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91808.1"
                     /translation="LGGVAILAEIARREFALHTVTMLLGATAWLTGNLLWMFGWQINQ
                     VVFFWMAFLVLTIAGERLELSRVLRPTQMQQILFGSIVFIFLAGIILSLFNLQFGTRL
                     SGLGLLLFPLWSLRNDIAWRNIRHKLPLTRYIAWCLALGFLWLGISGGLSLGLGAQVA
                     GPRYDAMLHTVFVGFVISMIFGHAPIIFPAILGIPINFQPVFYIHLGLLHISLLLRVI
                     ADYANLFTLRMWGGLLNEIAIFIFISLTVVSVRKGMIGKAR"
     gene            913..>1317
                     /locus_tag="PL000_23045"
     CDS             913..>1317
                     /locus_tag="PL000_23045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91809.1"
                     /translation="MKKYILLLLTLTLLIPTASVLAEGQFDYIVIRGFGIVEDINISN
                     PALTQDYYAFADFSKGEVNPPSDPGIGYQVVRMYAKDSKGIPYDQLHYYPDSGYVFYD
                     GIVNGYSELGTKWYLANPTVEEPFRAALAENAR"
BASE COUNT          293 a          319 c          300 g          405 t
ORIGIN      
        1 ctcggcgggg ttgccattct ggcggagatc gcccgccgcg aatttgcatt acacactgtc
       61 accatgttac tcggtgcaac tgcatggctg acaggaaatc ttttatggat gttcggctgg
      121 cagatcaatc aagttgtatt tttctggatg gctttccttg tgctgaccat tgcaggtgaa
      181 cggctcgaac tcagccgtgt tttgcgtcct acacaaatgc aacagattct ctttggcagt
      241 attgtgttca tttttctggc aggcattatc ctatccctgt ttaatctgca attcggcaca
      301 cgcctgagcg gactgggctt gctgttgttc ccgctttggt cgctgcgtaa cgatatcgcc
      361 tggcgcaaca ttcggcacaa acttccgctc acgcgctata ttgcctggtg cctcgcactt
      421 ggctttcttt ggctcggcat cagtggcgga ttaagcctgg ggcttggcgc gcaggttgca
      481 ggtccaaggt atgatgcgat gcttcacacg gtttttgttg gcttcgtcat ttctatgatc
      541 tttggtcacg cccccattat ctttcctgct attttgggaa tcccgatcaa ctttcagcct
      601 gtattttata tccacttggg gcttttgcac atttcgctgt tgctgcgcgt gatcgctgat
      661 tacgccaatc tcttcacatt gcgtatgtgg ggtggattgt taaatgagat tgccattttt
      721 atttttattt cactaacggt tgtctcagtg cgaaaaggca tgatcggaaa agccagataa
      781 ttttcaagca aatctcgatt catctttgct taagcgcaaa gatgaatttt ttatttcagg
      841 gataattcaa tcacaaccct attaagcgga caggttttca catacaatag accaaattaa
      901 aactggagac ttatgaaaaa atatatcttg ctcttattaa ctctaacctt gctgattccc
      961 accgcctccg ttcttgccga gggacaattt gactacatcg tcattcgcgg gtttggaatt
     1021 gtcgaagata tcaacatatc caaccctgcg ctgacacagg attattatgc ttttgctgat
     1081 ttttccaaag gcgaagtcaa cccgccctcc gaccctggaa ttggttatca agtcgtccgt
     1141 atgtatgcca aagacagcaa gggcattccc tacgaccaat tgcattatta ccccgacagc
     1201 ggatatgttt tttacgacgg catcgtgaat ggatattctg aattgggcac gaaatggtat
     1261 cttgccaacc ccacggttga agaacctttc cgcgcggcgc tggctgaaaa tgcgcgg
//
LOCUS       DAOQLC010000405         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_88040_length_1310_cov_20.985657, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000405 DAOQLC010000000
VERSION     DAOQLC010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88040_length_1310_cov_20.985657"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..>1310
                     /locus_tag="PL000_23050"
     CDS             247..>1310
                     /locus_tag="PL000_23050"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91810.1"
                     /translation="MALCGVKFKAHPTQEQKRVLSQWMGAGRFIYNAKVVEHEYFRTF
                     KAHSLALVGVPVPVDQCYAQFKTEQTLWLKDIPSQVLRNAAYRWREAYQRHLVGLAER
                     PVRKRKGNRDSVWLTSELFELNPQRDEQTGDVTGYQLVIGTKTNQIGALSFDARREFQ
                     SPHSITVSRRNGEYFVSFNYEDGQEVATEEELQAEYSQMDEASLDPITVGLDRGVVIP
                     VYPSEGEPFEFTPEQKHTLDHCDRHINRQQKRLSRQVKGSRRRERTRRKLARYHARKA
                     DVRRDFAHQTSHRLATSDHQIFVVEDLKIKNMTASPTPQPTADGSGYEPNGATAKAGL
                     NRSILAVAWGMVVTFLKYKAL"
BASE COUNT          365 a          327 c          382 g          236 t
ORIGIN      
        1 ttgaaccgaa cgacgagcgc aatctcggcc tttcaaggcc gagtcaagcg agtccgatct
       61 ggttttgaga atcaaagaac gagagcccaa ggagaaccag atggatcaca ggaaaaaaat
      121 cagccgctgg aaacaccatt gcgcaccgga aagtgccagg gggatgagat tgtgatcgac
      181 gggaagcgaa acagcatggt atcctgcaag ctattactgc cgtaataata cagatcgaga
      241 acaacgatgg cactctgtgg cgtcaaattc aaagcgcatc caacccaaga acagaagcga
      301 gtgttgtcgc aatggatggg tgctgggcga tttatctaca atgcaaaggt ggtggaacac
      361 gagtattttc ggacgttcaa ggcccacagt ctggcgctgg tcggggtgcc ggtgccggtg
      421 gaccagtgtt acgcccagtt caaaacagaa cagactctct ggctcaaaga catcccgagc
      481 caggtgttgc ggaatgcggc gtatcggtgg cgggaagcgt accagcgaca tctggtggga
      541 ctggcggaga gaccagtcag gaaacggaaa ggcaaccggg attcagtctg gctgacgagc
      601 gaactatttg agttaaaccc acaaagggat gaacaaaccg gggacgtgac tggctaccaa
      661 ctggtgattg gaacgaagac caatcagatt ggagcattga gttttgatgc ccggcgggag
      721 tttcaatcac cccattcaat cacggtgtca cgtcgaaatg gtgagtattt cgtgtcgttc
      781 aactatgagg atggccagga agtggcgacg gaggaagaac tccaggccga atacagtcag
      841 atggatgagg ccagcctgga ccccatcacg gttgggctgg atcgtggggt ggtgattcca
      901 gtctatccat cagaaggtga accgtttgag tttaccccgg aacaaaagca tacgcttgac
      961 cactgtgacc gacacatcaa ccgccagcaa aaaagactgt cgcgacaggt caaagggtct
     1021 cgccgtcggg aacggaccag acgaaaactg gcacggtatc acgcccggaa agctgatgtc
     1081 cggcgggact ttgcccacca gaccagccac cggctggcga cctccgacca ccagatattt
     1141 gtggtcgaag atctcaaaat caaaaacatg accgccagcc caaccccgca acccacagcc
     1201 gatggaagtg gctatgaacc caacggcgca accgccaaag ccggactcaa tcgaagtatt
     1261 ttggcggtgg cctggggaat ggtggtgaca tttctcaaat acaaggcgct
//
LOCUS       DAOQLC010000406         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_88438_length_1307_cov_12.519169, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000406 DAOQLC010000000
VERSION     DAOQLC010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88438_length_1307_cov_12.519169"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1089)
                     /locus_tag="PL000_23055"
     CDS             complement(118..1089)
                     /locus_tag="PL000_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017846754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNC91811.1"
                     /translation="MKHYRHLTEVERYQIEAHLRAGMNFAGIAMHMDRPRSCISREVN
                     RNGGRAHYRAQHAQARYREHLRSKGKLRQRWHAAFHEVAVPLLREGWSPEQISGVFRG
                     SECAVSHEWLYQRILQDKQSGGLVYRHLRCQKQRKKRYGSPERRGQIINRRGISERPA
                     EVESRQQLGHWEGDTMIGAGHRGALVTLVERTSGLVKIQRVTNKEAKGVTRAIHRMLS
                     PHRSKVHSITFDNGKEFAGHETIARLLKADCYFADPYSSWQRGSNENTNGLIRQYFPK
                     KTDFTTLSDKEIAEVERKLNNRPRKRLGFRSPNQVFNEGNFLNQKTS"
BASE COUNT          274 a          364 c          331 g          338 t
ORIGIN      
        1 tcagaaagca aattccagca tataaaaatg atgagtcttg gaattatgca tgggctcaaa
       61 ggtaaaatgg ggcgctttag caaaaaggct cgcggattca aaaagtaagt gcaacagtca
      121 gctggttttc tgattcaaaa agttaccttc gttgaacacc tgattgggcg atctgaaacc
      181 cagtcttttt ctcggccgat tattcagttt tcgctccact tccgcaatct ccttgtcact
      241 caaagtcgtg aagtcggttt tcttcggaaa gtattgccgg attaatccat tggtattctc
      301 attgctaccg cgctgccagg atgagtacgg gtcggcaaag taacaatccg ctttcagcag
      361 gcgggcaatc gtttcatggc cagcgaattc tttgccatta tcgaaggtga tgctgtgcac
      421 cttgctgcga tgcgggctca gcatccggtg aatggcccgt gttacacctt tggcttcttt
      481 gttggtcacc cgctggattt tgaccaggcc gctggtgcgt tccaccagcg tgactagtgc
      541 tcctcgatgg ccggcaccaa tcatggtgtc tccctcccag tgccccagtt gctggcggct
      601 ttcgacttcg gcaggccgtt cgctgatgcc acgccggttg atgatctgac cgcgccgctc
      661 cggtgagcca tagcgcttct tgcgttgttt ctggcaacgc aggtggcgat aaaccaagcc
      721 accggattgt ttgtcctgca agatgcgctg atacagccat tcatggctga cggcacattc
      781 gctaccgcga aacacgccgc tgatctgttc cggactccag ccctcacgta acaatggcac
      841 cgccacttca tggaatgccg cgtgccagcg ctgacgcagt ttgcctttgc tgcgcaagtg
      901 ttctcgataa cgcgcctggg catgttgcgc ccggtaatgg gctcttccgc cattgcgatt
      961 cacttctctg ctgatgcaac tccgggggcg atccatgtgc atcgcaatcc cggcgaaatt
     1021 catccccgcc cgcaaatggg cctctatctg gtatcgttca acttcggtga gatggcggta
     1081 atgcttcatt cgtcgtgctc ctgactggcc ggtcaaagtg cgacgaaatc taccccattt
     1141 cgccgacccc ttcccgattg ctgtcagttg cacttactcg ttgaatccgc ccaggaagat
     1201 ccagcctacc accggcgtac agatagattt ccggtattct taaaagcggg atcaaaggca
     1261 gatgtgttat tacgcaaaaa gtagacacgg gcgttatccg tcaaatt
//
LOCUS       DAOQLC010000407         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_88552_length_1306_cov_22.500400, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000407 DAOQLC010000000
VERSION     DAOQLC010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88552_length_1306_cov_22.500400"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1190
                     /locus_tag="PL000_23060"
     CDS             87..1190
                     /locus_tag="PL000_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNC91812.1"
                     /translation="MRSKDAKQQVFFSYKSIEDRIPQDHPIRTLKVLVDDILARLSPE
                     FDRLYSHTGRPSIPPEQLLRALLVQIFFSVRSERQLVEQLNYNMMFRWFVGLNLDDDI
                     WNATTFTKNRDRLLEGEVSERFFAEVLATAKKQGLVSQEHFTVDGTIIEAWASHKSFQ
                     RKEESGDDDDNTPSGDGGSRKNPDVNYKGQKRKNDTHESKTDPDARLCRKSQRTGAQL
                     GYMGHALMENRNGLVVDVRVTHAAGKAEQMAAASMASAIKGKHRVTLGGDKGYDSDEL
                     LGELRDMGVTPHIAKNDHERRTSSIDNRTTRHDGYDVSQKKRKLVEQIFGWGKTVGCL
                     RKVKHRGLDLVKSITTLNMAAYNLIRIRNLMRA"
BASE COUNT          386 a          295 c          347 g          278 t
ORIGIN      
        1 caggatgctg aaaaactcat agagtttgcc ccaataatag cagtatgtga gatcattgca
       61 tggtcagcgg gattggagac ttcgcagtga gaagcaaaga cgcaaaacaa caggtatttt
      121 tcagttacaa gtcgatcgaa gatcgaattc cgcaagacca tccaatacgg accctcaaag
      181 ttttggtcga cgatatactt gcgagattgt cgccggagtt tgatcgcctt tactcgcata
      241 ctggtcgtcc gtcgattccg cccgagcaac tgctacgagc gctactcgtt cagattttct
      301 tctctgtgcg gagtgagcgc caactcgttg agcagctgaa ttacaacatg atgtttcgtt
      361 ggttcgttgg ccttaacctg gacgatgata tatggaatgc aacgacattt accaagaacc
      421 gtgatcgctt gctggagggg gaagtttccg aacgattctt cgccgaagtg ctggcaaccg
      481 cgaagaagca gggtctcgtc tcacaagaac atttcacagt tgacgggacc atcatcgaag
      541 cttgggcaag tcacaaaagc tttcagcgca aggaagagag tggcgatgat gacgacaata
      601 ctccgtccgg tgatggcggc agtagaaaga atccagacgt aaattacaaa gggcagaaga
      661 ggaagaatga tacccatgaa tccaaaacag atccggatgc tagactctgc cgcaagtctc
      721 aaagaactgg agcgcagctt ggatacatgg ggcatgcgct gatggagaat cgcaatggac
      781 tcgttgtcga tgtacgggta actcatgcag caggaaaggc agagcaaatg gcggcagcca
      841 gtatggcaag cgcaatcaaa ggcaagcatc gcgtaactct tggcggagat aagggatacg
      901 actccgatga gttgcttggc gagctgcgag acatgggtgt aactccacac atcgccaaga
      961 atgaccatga acgcagaacc agttcgatag ataaccgcac aactcgccat gatggctacg
     1021 atgtaagcca gaagaaacga aaactcgtgg agcaaatatt tggttggggc aagactgttg
     1081 gatgcttgcg caaggtaaaa cacagagggc tggacctggt caaatcaatc acgactctga
     1141 acatggcagc ctacaatttg ataagaatca ggaatctgat gagggcttaa aggttgccaa
     1201 tgagcaaccg agtacccagt atttacaaac tcaagactgc cggctcgatt taacgaaatc
     1261 ctggaaggga gccttgccac tacaaacgat gtttttcagc accctg
//
LOCUS       DAOQLC010000408         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_89476_length_1299_cov_27.879421, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000408 DAOQLC010000000
VERSION     DAOQLC010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89476_length_1299_cov_27.879421"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..1283)
                     /locus_tag="PL000_23065"
     CDS             complement(171..1283)
                     /locus_tag="PL000_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNC91813.1"
                     /translation="MPSLLDHFSDLPDPRVDRTKLHLLIDIVTIALCAVICGAETYEE
                     METFGESKHEWLATFLELPNGIPSHDTFRRVLMRLNPKEFESRFLTWVSCVADLTQGE
                     IVSIDGKTLRGSRSKSNSTIHMVSAWAAANRLVLGQVKTEDHSNEITAIPELLSVLML
                     KGCIVTIDAMGCQTEIAGQIHEQDADYVLTLKANQPTTHQAVISFFDQARLAPGPTPA
                     LSFFQTVDNDHGRLETRRCFAAPASAALDPELLARWPGLTSVVKITATRDIDGDRSTE
                     SRYFLSTLPPDAQHLLSVVRTHWQIENCLHWVLDVAFREDFSRVRSGHAPHNFAILRH
                     IALNLLKQETSCKNGIKSKRLKAGWDHQYLLKVINS"
BASE COUNT          291 a          321 c          414 g          273 t
ORIGIN      
        1 ccagcggcgc aaaccggttc tggctttgct ccaacccttc ctgatgaagt cggttcagtt
       61 tgaccgttgg aaaccggact ttgagttcat atccccagaa ggaccaccca aactggtttg
      121 gattccagtc cggagtggag tctcccagca ccaccaaggg taaacgcacc ttaactattt
      181 atgactttta ggagatactg gtgatcccac cccgccttca agcgtttgga tttgatgccg
      241 tttttgcagg aagtctcctg tttgagcaaa ttgagggcga tgtgccgaag aatggcaaaa
      301 ttgtgaggag cgtgaccgga acggacgcgg gagaaatctt cgcggaaggc gacgtcgaga
      361 acccaatgga ggcaattttc aatctgccag tgagtgcgca cgaccgagag gaggtgctga
      421 gcgtcaggcg gcagggtgga aagaaaatag cgggattcgg tcgagcggtc accgtcaatg
      481 tcacgggtgg cggtaatttt gacgaccgag gtgaggccag gccagcgggc gaggagttcg
      541 ggatcaaggg cggcactggc tggggcagca aaacagcgac gggtttcaag ccgaccgtgg
      601 tcattatcga cggtttggaa aaaagaaagg gctggggttg ggccgggagc gagtcgggcc
      661 tggtcaaaaa aggagatgac cgcctgatgg gtcgtgggct ggttggcttt gagggtcaag
      721 acatagtcgg catcttgctc gtgaatctgg ccggcaattt cggtctggca gcccatggca
      781 tcaatggtca caatgcagcc cttgagcatc aggacggaga gcagttccgg gatggcggtg
      841 atttcgttgg aatggtcctc ggttttgacc tgacccagga ccaggcggtt ggcggcggcc
      901 caggcgctga ccatgtgaat ggtggagttg gacttgctgc gcgacccacg cagggttttt
      961 ccgtcaatgc tgacgatttc gccctgagtg agatccgcca cacagctcac ccaggtcaga
     1021 aaccgcgact caaactcttt tggattgagc cgcatcagca cccgtcgaaa ggtatcgtgg
     1081 gacgggattc cgttgggtaa ctcaaggaag gtcgccaacc actcgtgttt cgactcgccg
     1141 aaggtttcca tttcctcgta ggtctcggcc ccgcagatca cggcgcacag cgcaatggtc
     1201 acgatatcga tcaacaggtg aagtttcgtc cgatccaccc gcggatcggg caggtctgaa
     1261 aaatggtcaa gtaaggaagg catgcgttga ctccaggct
//
LOCUS       DAOQLC010000409         1290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_90759_length_1290_cov_12.191093, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000409 DAOQLC010000000
VERSION     DAOQLC010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90759_length_1290_cov_12.191093"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..>1290
                     /locus_tag="PL000_23070"
     CDS             381..>1290
                     /locus_tag="PL000_23070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91814.1"
                     /translation="MSDKVKAYKGFNRDWTCRGFQYEMGNTHVFDGEVVMCWSGFHAC
                     TRPMDVFWYYHPGFSVFAEVELAGEICSELEGCDSKLASKEITVVRPLSLLQMADIAV
                     VEIDKDLSRTNDHFGYANHTRYCGMDPFSFAFSAGAGCYVETTERQSSATVIGERSAA
                     IAIRDQSVASSIGQQSVSMCFGDHSVATTHASSSIAVSKSLGCASLSLCSGSAAVSTN
                     VDSVAVVMGSLSAAESHGDNSVALATRTLSAASVSGIGSVAIALGQRCVAKAEEGGSI
                     VVAHISSYGHITAIRASLVGQNGIEPG"
BASE COUNT          283 a          287 c          368 g          352 t
ORIGIN      
        1 caaaaagcca gcaaagcctg tggcaaatat cccacagcgg ggttattgca acccacctga
       61 taccaatgtc gctgagaagc aaccaaagaa gatgcgcgtg cctcgcggca cggcccgtgc
      121 tttgcgtcgt gctgcagcac gggaagcggc agaacgtgac caagcttgag aacggcaaaa
      181 tcccagctta cactccatgc ccttttcgcg gggagtgtgg tagcgcaaag cttggtctct
      241 gccatcattt tggtgaatac cacagggtag ccttttcgtg tggtacagca cgcttgttcg
      301 accgcctact tgtgtgggca ttttgggcag tcatattggg ttatccgatg tggttgcatt
      361 tcttttattg gaggtgaaaa gtgtctgaca aagtgaaggc ttacaaaggt ttcaatcgag
      421 attggacctg tcgtggtttt caatacgaga tgggtaacac ccatgtgttt gatggcgaag
      481 tagttatgtg ttggtcaggt tttcacgcat gcacccggcc catggatgtg ttttggtact
      541 atcacccagg tttcagtgtt tttgcagaag tagagcttgc tggtgagatt tgttcagaac
      601 tggagggctg cgacagcaag ttggcaagca aagagattac cgttgttcga cctttgtcgt
      661 tgcttcaaat ggcagacata gccgttgtag aaattgacaa agacctctcc cgaacaaacg
      721 accactttgg ttacgctaac cacacccgtt attgtggcat ggatccgttt tcgtttgcat
      781 tttccgcagg ggctggttgt tatgttgaaa caaccgagcg acagtcttcg gcaacggtaa
      841 ttggtgaaag gtctgctgcc atcgccatac gtgatcaaag tgtggcttcg agcattggac
      901 agcaatctgt ttccatgtgc tttggcgacc atagtgttgc gacaacacac gcctcgtcaa
      961 gcattgccgt gagcaagtcg ttgggttgcg catctctgtc tctctgtagt ggttcggctg
     1021 ctgtatccac gaatgttgat tctgttgctg tggtcatggg ttcgttgtct gcagcagaat
     1081 ctcatggtga caacagtgtg gctttggcca ctcgcacgtt gagtgcggca agtgttagcg
     1141 gtattgggtc tgttgcgatc gcgctggggc aaagatgcgt agccaaggca gaagagggtg
     1201 gttctattgt cgtggcgcat atttccagtt atggacatat cacggcgatt cgagccagtc
     1261 tagttggcca gaatggcatt gagccgggta
//
LOCUS       DAOQLC010000410         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_91161_length_1287_cov_29.425325, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000410 DAOQLC010000000
VERSION     DAOQLC010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91161_length_1287_cov_29.425325"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="cimA"
                     /locus_tag="PL000_23075"
     CDS             complement(<1..926)
                     /gene="cimA"
                     /locus_tag="PL000_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNC91815.1"
                     /translation="MTLPLIQIYDTTLRDGTQSEGFNLSANDKVRVAQKLDEFGVSFI
                     EGGWPGSNPKDVEFFERARDMQWKNALITAFGSTCRVKGGPEDDANIKALLDSQTPVC
                     TIFGKTWTLHVTEVLQTTYEDNLRIIEDSVAYLKANGKRVIYDAEHFFDGYKADSSYA
                     LETLKAAIRGGAEMVVLCDTNGGSMPWEVEQVIREMKAQIQHPFGIHPHNDSETALIN
                     ALTAVREGAVQVQGTINGVGERCGNVNLCSVVANLELKMGYQCLPKGNIQHLYDLSHF
                     VAEVANLTPDEHLPFVGKSAFAHKGGVHVAAMR"
     gene            complement(985..>1287)
                     /locus_tag="PL000_23080"
     CDS             complement(985..>1287)
                     /locus_tag="PL000_23080"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC91816.1"
                     /translation="GIRAVISTSFADIFRNNSLKNGLIPIIVDEATHKMLFDLVEEAP
                     RAELTVDLATQTVTFPGGSFTFPIDAFNKTCLLNGVDELGYIMGFEKDIAAFEAKA"
BASE COUNT          277 a          326 c          366 g          318 t
ORIGIN      
        1 cgcatcgccg ccacatgcac accgccttta tgcgcgaacg ctgatttacc gacgaagggc
       61 aaatgctcgt cgggtgtgag gttggcaact tctgccacga agtgcgagag atcatacaag
      121 tgctggatgt tgcccttggg caaacattga tagcccatct tcagttcaag gttcgccaca
      181 accgagcaca ggttgacgtt gccgcagcgt tctcccaccc cgttgatggt tccctgtacc
      241 tgaactgcac cttcgcgaac agcggtcagc gcattgatga gggccgtctc cgaatcattg
      301 tgcggatgaa tgccaaaggg atgttgaatc tgcgccttca tctcgcggat gacctgttcc
      361 acttcccacg gcatcgaacc gccgttggta tcgcagagca caaccatttc tgcgccgccg
      421 cgaatggcag ctttcagcgt ttcgagcgcg taggacgaat ccgctttgta gccgtcgaag
      481 aagtgttcgg catcgtagat gacacgtttg ccattggctt tgaggtaggc gaccgaatct
      541 tcgatgatgc gcaggttgtc ttcgtaagtg gtttgcaaaa cttcggtcac atgcaatgtc
      601 cacgttttgc cgaagatggt gcagacgggg gtttgcgaat cgagcagggc tttaatgttg
      661 gcatcgtctt cgggtccgcc tttgacgcgg caggtggaac cgaacgccgt gatgagggca
      721 ttcttccact gcatgtcgcg ggcacgttcg aagaactcca catctttggg gttcgagccg
      781 ggccagccgc cttcgatgaa ggacacgccg aactcatcta atttttgcgc cacacggacc
      841 ttatcatttg ccgaaaggtt gaagccttcg gattgtgtcc catcgcgcag ggtggtgtcg
      901 taaatttgaa taaggggtag ggtcatgtgt tgccttttgg tgattggtaa gtagtaactg
      961 taattaccaa tcgccaatta ctaattacgc ttttgcctca aacgccgcga tgtctttttc
     1021 aaaacccatg atgtagccga gttcatccac gccgttgagc aaacaagttt tgttgaaagc
     1081 atcgatcggg aaggtgaacg agcctccggg gaaggtcacg gtttgggtgg caagatcaac
     1141 tgtcagttcg gcgcgaggcg cttcttccac caggtcgaaa agcatcttgt gagtcgcttc
     1201 atccacaatg atgggaatga gtccattctt cagcgagttg ttgcggaaga tgtctgcgaa
     1261 cgaggtggag atgacggcgc ggatgcc
//
LOCUS       DAOQLC010000411         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_92441_length_1278_cov_21.506132, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000411 DAOQLC010000000
VERSION     DAOQLC010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92441_length_1278_cov_21.506132"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1259
                     /locus_tag="PL000_23085"
     CDS             105..1259
                     /locus_tag="PL000_23085"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNC91817.1"
                     /translation="MSQSTVFSQIIKLIPRAEFQAIVHKHNGDKGLRSLDCWTWFGSL
                     LFGQLTGHDSIRAIERIFATSDSKMHKLGFGPVRRSTLADANRTRPLEVLEDLFQYTL
                     GRAQRCAPKKNGFRFHGQVLALDSTTIELCLSLSPWAKFHHDKGATKLHTAIDIAGDL
                     PLFTVITDGRIHDIRAAREQIQFQPGTTVVFDRGYVDYSWLNNLNQSHVWFVTRTKSN
                     CRFKVVESRPTDRTRGHICDQVIYLKSQRGGEYEGKLRRITFKDPATGKRLTFLTNRF
                     DLATQTICDLYKARWKVELFFKTMKQYLRVKKFLGISANAVKAQILVALIAYLLVQIL
                     RFTIKTSISIADAMAVVGTLLLLNEPLKRLLGNLPRVTRHQREFQMVFNL"
BASE COUNT          329 a          343 c          307 g          299 t
ORIGIN      
        1 ttcactactg tccggttcgg agtggggcat aaaaatctaa gtatctgaaa acactctgtg
       61 gtatgaggtt tttaccacaa aacaacttcc cacagaggtt tcagatgagc caatctaccg
      121 tattttccca gattattaaa ctcattccgc gcgcggagtt ccaggccatc gtccacaaac
      181 acaatggtga caaaggtctc cgatccctgg attgttggac gtggtttggg tccctgctat
      241 ttggtcaact caccggacac gactccatcc gggccataga gcggatattt gctacgagcg
      301 attccaagat gcacaagctt ggctttggcc cagtcaggcg cagcacgctg gccgatgcga
      361 accggacacg cccactggag gtccttgagg acctatttca atacactctg ggtagggccc
      421 agcggtgcgc cccaaagaaa aatgggtttc gcttccatgg ccaggtgttg gcattggact
      481 caacgacaat tgagctgtgc ctgagcctgt ctccctgggc taaatttcac cacgacaaag
      541 gcgctaccaa gctccacaca gctattgata ttgcgggcga tttacccctg tttaccgtca
      601 tcaccgacgg ccgcatccat gatattcgag cagcccgaga acagattcaa ttccagcctg
      661 gtacaaccgt tgtctttgac cgtgggtacg tcgactactc gtggcttaac aacctcaatc
      721 agtcccacgt ttggtttgtt acccgcacca aatcaaattg ccgattcaag gttgtcgaga
      781 gcaggcctac ggaccggaca cgaggccaca tttgcgatca ggtgatttat ctgaagagcc
      841 aaaggggcgg cgaatacgag gggaagcttc gtagaataac attcaaagac ccagcaacag
      901 gcaagcgact gacatttcta acaaaccgct ttgatctggc aactcagacc atttgcgacc
      961 tttacaaggc tcgctggaaa gtggaacttt tcttcaaaac aatgaagcag tacttgcggg
     1021 tcaagaaatt ccttggaatc agcgccaatg cagtgaaagc gcagatcctc gtcgcactga
     1081 tcgcatacct actcgttcaa attttgcgct tcacaataaa gaccagtatc tcaatcgccg
     1141 atgccatggc ggttgtcgga actctgcttc tactaaatga gcctctgaag cggcttctgg
     1201 gcaacttgcc ccgcgtaact cgccaccaga gggagtttca gatggtattc aacctataaa
     1261 atgagccgga cagtagtg
//
LOCUS       DAOQLC010000412         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_93123_length_1273_cov_27.142036, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000412 DAOQLC010000000
VERSION     DAOQLC010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93123_length_1273_cov_27.142036"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..977)
                     /locus_tag="PL000_23090"
     CDS             complement(360..977)
                     /locus_tag="PL000_23090"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNC91818.1"
                     /translation="MRESVFDPQAQIGNPDLKIIASLERLSEGFRVLLWEKAKILGIS
                     PIQIQILIFIRFHSDEKCTVSYLAQEFCLTKPTVSEAVKSLEQKGFIERYTNIMDTRS
                     HTIHLTDTGKSAVARTADFANPMLGSIAGMQPADKGILLEQLLSVVSHLQGVGIISMQ
                     RMCFSCRFYQRNNNGHFCGFLNKSLKNHELRLDCGEFESELLQHV"
     gene            complement(958..>1273)
                     /locus_tag="PL000_23095"
     CDS             complement(958..>1273)
                     /locus_tag="PL000_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91819.1"
                     /translation="KGDSSGPNPPAFLTRNRWTKCSVQIVLSNFGCGFLSHASAIFSP
                     DALHQTQKSPRLIQKSTTQNSTNYLYRTLSFYKKPYQLKKYLFLPLCPHRNESTHERK
                     RI"
BASE COUNT          303 a          285 c          354 g          331 t
ORIGIN      
        1 cttgggccgg cacggagcct ttggctatgt ggtgttccac aagccgtagc agaccgatgc
       61 aggcataaga gcctgtctag gttttcctga tcgagcgaaa gcgaggaaat tcggtttcgg
      121 gcaaggcggg tttcacagac ttatccggcg gctacggcga agaaaaccaa cgcaggccca
      181 aacgaaattt gccgcttcga gccgattgag gaaatcctac tacatgctct aaacacggag
      241 gggcgggtac gattcatcgt atccgcccct ccgtgtttag agcacatgtc tggattttca
      301 cctgcttttt gattcgaaaa cgttgggcaa aagagttgct tttgatcaat cggggcaatt
      361 tagacatgct gcaacagttc cgattcgaac tctccacaat ccagccgcaa ctcatggttc
      421 ttcaatgatt tgttcaggaa tccgcagaag tggccattgt tgtttctttg gtaaaagcga
      481 caggagaaac acatcctttg catggatatg atgccgaccc cttgtaaatg gctcactacg
      541 ctgagcaatt gttcgagcag gatgccctta tcagcgggct gcattccggc aatgctgccg
      601 agcatcggat tggcgaaatc ggcagtgcgg gccactgcgg attttcccgt gtccgtcagg
      661 tggatggtgt ggctacgggt gtccatgata ttggtatacc gctcgataaa tcccttctgt
      721 tcgagcgatt tgaccgcctc gctgacggtg ggcttcgtca ggcaaaactc ttgggcgagg
      781 taactgaccg tgcatttttc atcgctatga aatcggataa aaatcagaat ctggatctgt
      841 atcgggctga tccccagaat ctttgctttt tcccatagca atacacggaa gccctcgctg
      901 agccgctcga gggaggcgat gattttcagg tcgggattgc cgatttgggc ttgggggtca
      961 aatacgcttt ctctcatgag tagactcgtt gcgatggggg caaaggggca aaaacaggta
     1021 tttcttcagc tgataaggct ttttgtaaaa acttagtgtt ctgtacaagt agttggttga
     1081 attttgggta gtggattttt ggatcaggcg aggcgatttt tgagtttgat gcagcgcatc
     1141 gggcgaaaaa atggctgaag catgggacaa aaagccgcag ccaaaatttg acagaactat
     1201 ttggacagaa cacttagtcc agcggtttcg ggtcagaaaa gcaggcggat ttgggccaga
     1261 tgagtcgcct ttt
//
LOCUS       DAOQLC010000413         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_93253_length_1272_cov_19.184059, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000413 DAOQLC010000000
VERSION     DAOQLC010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93253_length_1272_cov_19.184059"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1161)
                     /locus_tag="PL000_23100"
     CDS             complement(142..1161)
                     /locus_tag="PL000_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91820.1"
                     /translation="MKTLHQRITPVILGLLSFLLFAFVPLSKTHGMVRESAGGKADGP
                     DVYYVYITVLNISNIDLSAGTYAVDFFISFICETPPCQYEPHWDVMNATEIIDPEDQG
                     TSIPFEVYDYRLKTSLIGYVDYTFYPFDYLYVDIIIEDKEYSNDELVYRSAAVEVDPM
                     LFNPSGWYYRPEYDGDMNDIVVYPNDDISYDRLYIWLFMERDRFSGFMKTIFAALVIV
                     LVGMLSFLMKAEAAGERLALVSSTLVAVVLYHISLVSGVPATGYLTFIDKFMIGTYAV
                     VFVSLVISVAMMVYKNNDELGKAEKLHLRTRWIIPLLWVFLMVCVFIVELILPYRQML
                     AANGG"
BASE COUNT          407 a          317 c          250 g          298 t
ORIGIN      
        1 ccagtgtata cattccagaa gcctccaaag atgcctggga acgtgcgaaa aaattcttca
       61 gcaagaactt aaattagtca ctttccatcc aggaaacaaa aaacagccca tttttcaaat
      121 gggctgtttt tgttctgagc gctaccctcc gtttgctgca agcatctgcc tgtaaggcaa
      181 aattaactct acgataaata cacacaccat cagaaatacc catagcaatg gaatgatcca
      241 acgagtacgc aggtgaagct tttcagcctt ccccaattca tcattattct tgtaaaccat
      301 catggctact gaaatgacca gcgaaacaaa caccacagca tatgtaccga tcataaactt
      361 atcaatgaaa gtcagatacc cagtggcggg cacccctgac accaatgaaa tgtgatacaa
      421 cacaaccgcc accaaagtgg atgagaccaa tgcaaggcgt tcgccagccg cttcagcctt
      481 catcaaaaac gacaacatac cgaccagtac gatcaccagg gcagcaaaga tcgttttcat
      541 aaaaccgcta aagcggtcgc gttccatgaa cagccagata tacaagcggt cataggaaat
      601 atcatcgttt ggataaacaa caatatcgtt catatcgcca tcgtattcag ggcggtaata
      661 ccagcctgac gggttaaata gcatcgggtc cacttcaacc gcggcggacc gatataccaa
      721 ctcgtcattg ctatactcct tgtcttcgat gatgatatcc acataaagat aatcgaacgg
      781 gtaaaacgta taatccacgt acccgatcag ggaggttttc aagcgatagt catatacttc
      841 aaaaggaatt gaagtcccct ggtcttccgg gtcgatgatt tcggtggcgt tcattacatc
      901 ccaatgcggc tcgtattggc atggcggggt ctcacatatg aatgaaataa aaaaatcaac
      961 cgcgtacgtg cccgctgaaa ggtcaatatt ggaaatgttg agcacagtga tgtacacata
     1021 ataaacatca gggccatccg ccttgccacc tgcagactca cgcaccattc catgtgtttt
     1081 ggacaaaggg acaaaagcaa aaagcaaaaa agacagcagc cccaaaataa cgggagtaat
     1141 tcgctgatgc aatgttttca tgttgcctcc agttaaaaaa ttatagagaa agtgtagcac
     1201 ggctcgtttg gcaaatcaat ttacatccag ttgacaaatc ttccgctctc ctttattctc
     1261 gcttcatggc ca
//
LOCUS       DAOQLC010000414         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_94400_length_1264_cov_16.394541, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000414 DAOQLC010000000
VERSION     DAOQLC010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94400_length_1264_cov_16.394541"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PL000_23105"
     CDS             <1..600
                     /locus_tag="PL000_23105"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNC91821.1"
                     /translation="ALADSPAQAEALASDYAKMREQQAEQIRVQVSQFIQQIRQEQNP
                     MSFDVSIKSIPVQQVLSTTHHIKASKLDETILKSLEAFRKLLAEQQLEAASAPFGIFH
                     GAVNEQEDGPLEICMPVNGAVKGNSKIQVKQLQGGQAACVMTVGPETDFPVILGAYDV
                     AADWIQKNGHEMAEPPREIWHSGPGGSVEPKMEIVWLFK"
     gene            652..1149
                     /locus_tag="PL000_23110"
     CDS             652..1149
                     /locus_tag="PL000_23110"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNC91822.1"
                     /translation="MTTIGKPKIEKRPKITYIGIRTIAPFKGMSKEIGRIADELNKWV
                     NENKIKTAGPPFLRYHVIDMRGFMDISFCVPVKKALPNDGNVKADIVPAGRYASLVFS
                     GGGISGNRALIEWVRAQGMDFDRWDTDQGDNFAGRYETYLTDPSVEPRKSKWKIEVAI
                     KLAGK"
BASE COUNT          357 a          322 c          311 g          274 t
ORIGIN      
        1 gcattggcag actcgcccgc gcaagccgaa gcgctggcaa gtgattacgc aaagatgcgt
       61 gaacaacaag ccgagcagat tcgggtacag gtctctcaat tcattcaaca aattcgacag
      121 gagcaaaacc ctatgagttt cgatgtcagt attaaatcca ttccggttca acaggtgttg
      181 agtactacac accacatcaa agccagcaaa ttagatgaga caattctaaa atcgttagaa
      241 gcattccgta agcttcttgc tgagcaacag cttgaagccg cgtctgcacc gtttggcatc
      301 tttcatggtg ctgtcaatga acaggaagat ggtccactag agatctgtat gccagtaaac
      361 ggagcagtca aaggcaatag caagattcaa gtcaaacagt tgcaaggtgg gcaagccgcc
      421 tgtgtgatga cggtcggacc agagaccgac ttccccgtca ttctcggcgc gtatgatgtc
      481 gctgccgatt ggatccaaaa gaacggacat gaaatggcag aaccaccgcg cgaaatctgg
      541 cacagcggac ctggcgggag cgtagagcca aagatggaga tcgtctggtt atttaagtag
      601 gggggtaccc tgcccctact taaaatttga attttattac ttggagcaca catgaccacc
      661 atcggcaaac ccaaaataga gaaacgccca aagatcactt acataggcat ccgcaccatc
      721 gcgcctttca aaggcatgtc caaagagatc ggcaggatcg cagatgaatt gaataagtgg
      781 gtaaatgaaa acaaaatcaa aacagcgggt ccgccctttc tgcgctacca cgtcattgat
      841 atgcgaggct tcatggacat ttccttctgc gtccctgtca aaaaggcatt gcccaacgat
      901 ggcaacgtca aagcagacat tgtcccagct gggcgatatg ccagcctcgt tttttcaggc
      961 ggcggcattt cgggcaatcg cgccctcatt gaatgggtgc gcgcccaagg catggacttt
     1021 gaccgctggg ataccgatca aggtgataat ttcgcaggtc gttacgaaac ctatctcacc
     1081 gacccaagtg tcgaaccacg caaatcgaag tggaagatcg aagtagcgat caaattggca
     1141 ggtaagtaaa aaagtaggtc acgcttatgg cgtgacctac tttttatttc aaagactttg
     1201 ccgtattccg tcccgccgcg ccgctgactc ctcctccacc atggactccg ctgccgcaga
     1261 gata
//
LOCUS       DAOQLC010000415         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_95147_length_1259_cov_24.132890, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000415 DAOQLC010000000
VERSION     DAOQLC010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95147_length_1259_cov_24.132890"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..782)
                     /locus_tag="PL000_23115"
     CDS             complement(123..782)
                     /locus_tag="PL000_23115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEPxxWA-CTERM sorting domain-containing protein"
                     /protein_id="HNC91823.1"
                     /translation="MSAFLKNRSALLGCAAVVSGFLVSLPAIAEPNFYSTGSHVSGAL
                     DLDWLVSTGYGSFNTSSFVQANTWVHGSETWISDTDYSQPVTKPQYQYFTFRQYFDLT
                     GYDASSAHLQFYWGADDVPGAVGYTPQFSINGGTFQGSGTATGYNIGTNLVDLTSGFV
                     PGMNYIDFRVEGNYATNGMGLKVVSFTAAVPEPGTYAMMLAGLGLVGFVARRRPMKQQ
                     S"
BASE COUNT          295 a          344 c          343 g          277 t
ORIGIN      
        1 gaacctggac ccgactgtca gtgttcgctg ggatgccctg accttgaacc tccactccag
       61 ctttatgagc aacaggaaat ggaaaaggcc accccgattg aggtggcccg tagcgggaga
      121 gatcaggatt gctgtttcat aggcctgcga cgagcgacga acccgaccaa ccccaaaccc
      181 gcaagcatca tggcgtaggt tccaggttcg ggcacggcgg cagtgaagga caccactttc
      241 aggcccatgc cgttggtcgc gtagtttccc tctactcgaa agtcgatgta gttcatcccc
      301 ggcacaaacc cggacgtcaa atcgacgaga ttggttccaa tgttataccc ggttgccgtc
      361 ccgctcccct ggaaggtacc gccgttgatc gagaactgcg gggtgtatcc gaccgccccc
      421 gggacatcat ctgcacccca atagaactgg aggtgtgcgc tcgatgcatc gtaccctgtg
      481 aggtcgaagt attggcggaa cgtgaagtat tgatattgtg gttttgtgac gggctgcgag
      541 tagtcggtgt cactgatcca agtctcgcta ccatgcaccc acgtattggc ttgtacgaag
      601 ctagaagtgt tgaagctacc gtaccccgtc gagacaagcc agtccagatc aagcgcgccg
      661 ctgacatgtg acccggtcga gtagaagttt ggctccgcaa tggcaggcaa agaaactaga
      721 aaacctgaga cgacagcggc gcaccccaat aaggcactgc gattcttcag gaaagcagac
      781 atttaaagtt cctttcttag gcgttgataa agctcattcg gcgtccatcg tccaatcatc
      841 aggctgattt gttcacgtcc gggatgtagc ttccgatacg ctgcaaagtg ccgaaattcc
      901 tatgccgccg cgccttcgcg tccactgtag gaatgtaagg tttcctgaca gtcccgatga
      961 agccgcgccg gcccctgatg ccgtttatca gattgatgca agtcgccgaa gtggtggctg
     1021 accaccagac tgacgtcttg cagcgaagtg acgctaagca cactgtcgta gaccgatccg
     1081 gtggctcggt cgtagggggt ggcgtgggcc acgctggaat acaaagagtg gccactttga
     1141 gttggctgct catctgttta ctcaagtaat tccataaatg gagctatcca gaactacgca
     1201 gtaaccctta agcaggaacg atgccgttac tcggattcct ttatcttcaa aaagatacg
//
LOCUS       DAOQLC010000416         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_95148_length_1259_cov_22.557309, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000416 DAOQLC010000000
VERSION     DAOQLC010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95148_length_1259_cov_22.557309"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..953)
                     /locus_tag="PL000_23120"
     CDS             complement(150..953)
                     /locus_tag="PL000_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-5 family class D beta-lactamase OXA-129"
                     /protein_id="HNC91824.1"
                     /translation="MKTIAAYLVLVFFAGTALSESISENLAWNKEFSSESVHGVFVLC
                     KSSSNSCTTNNATRASTAYIPASTFKIPNALIGLETGAIKDARQVFKWDGKPRAMKQW
                     EKDLTLRGAIQVSAVPVFQQIARDIGKKRMQKYLNLFSYGNANIGGGIDKFWLEGQLR
                     ISAVNQVKFLESLYLNNLPASKANQLIVKEAIVTEATPEYIVHSKTGYSGVGTESNPG
                     VAWWVGWVEKGTEVYFFAFNMDIDNESKLPSRKSIPTKIMASEGIIIGG"
     gene            1063..>1259
                     /locus_tag="PL000_23125"
     CDS             1063..>1259
                     /locus_tag="PL000_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91825.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAF
                     IRFRGSYHIAAKSAICIILSA"
BASE COUNT          321 a          295 c          270 g          373 t
ORIGIN      
        1 tctgatagtt ggttcgaaat ttcgatggtc accgctaccc tcatgatgtc taacgcaaag
       61 ctaaggggcg gctacaagcg gcgcgcaaaa tgcgaagcat gcgccgattg gagacgtcca
      121 gtggcgcagc cacggccttg agcgccttgt tagccaccaa tgatgatacc ttcacttgcc
      181 atgattttcg ttggaatgga ttttcttgac ggcaacttac tctcattgtc tatgtccatg
      241 ttaaatgcaa aaaagtaaac ctcagttcct ttttctaccc aaccaaccca ccaagcgaca
      301 ccaggatttg attctgtgcc cacaccggaa tacccggttt ttgaatgcac tatatattct
      361 ggagttgctt ctgtaactat tgcctctttt actataagtt ggtttgcttt agatgctggc
      421 aaattattta ggtaaagcga ctctaaaaat ttaacttgat tgactgctga gattctaagc
      481 tgaccttcta gccaaaattt gtcaatgcct ccgcctatat tggcgttgcc atatgaaaaa
      541 aggttaaggt atttttgcat tctttttttg ccaatgtctc tggcaatttg ttgaaatacc
      601 ggaacagcag aaacttgtat agcgcccctt agcgttaagt ctttttccca ttgcttcatg
      661 gctctgggct tgccgtccca tttgaaaacc tgccgcgcat cttttatggc gccggtttca
      721 aggcctatga gagcattggg aattttgaat gttgatgctg gaatataggc cgtagatgca
      781 cgtgttgcat tatttgttgt acaggaatta ctactgcttt tacaaagtac aaaaacacca
      841 tgcactgatt cactggaaaa ttctttattc caagctaaat tttcagaaat agactctgaa
      901 agtgcagtgc ctgcaaaaaa tactagaact aaatatgcgg ctatggtttt catggtacct
      961 tggtggctaa ctttgtttta gggcgactgc cctgctgcgt aacgcgcttg ctgcttggat
     1021 gcccgaggca tagactgtac aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     1081 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     1141 ttgcattaca gcttaccaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     1201 ttccggggta gttaccacat agctgctaaa agtgccatct gcatcatcct atcagcaga
//
LOCUS       DAOQLC010000417         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_95747_length_1255_cov_28.452500, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000417 DAOQLC010000000
VERSION     DAOQLC010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95747_length_1255_cov_28.452500"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1255)
                     /locus_tag="PL000_23130"
     CDS             complement(<1..>1255)
                     /locus_tag="PL000_23130"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNC91826.1"
                     /translation="ADAGKRAAVALIAALSDQSQSVAIQVLGDSTGNDTGEWPRLIAN
                     QLASDNPAWTVRTILFSDTTQKYSSPVVIQTGPDGERYFDGATGTSTRTLAASESPYI
                     SGTIDVSVKMRLASWSNPGAQVNVFGKSGTPGYRGWYGYINSTGIVYFAYSTDGTALV
                     TMAATNTGITDNTDYWVRWIFTPDDGAGNRVFRAYKSPNGETWTQIGSTVTTPGAVSV
                     YNNSTIGYEAGGVSTSAVVRVTRLYEIIIRDGEDGKIVAPVMPDLWYPKSSSSAYFTG
                     SPTLTVVNGSVSGAGITGAGNYLADATRLPKLTPNYGQVLCFLSTSHNDGYDHGRSYR
                     ATYGAWIDSVKELLPDVPICLLTQNPEKSGATWYREHAKRHLDLLTMEMNKSVVALDT
                     YQDFIDYQNWQTDYMADNVHPNSAGQ"
BASE COUNT          285 a          357 c          289 g          324 t
ORIGIN      
        1 ctgtcctgcc gaattcgggt ggacgttgtc tgccatatag tctgtctgcc aattttggta
       61 atcaataaaa tcctggtacg tgtccagcgc aacaaccgat ttgttcattt ccattgtaag
      121 caaatccaga tggcgtttgg cgtgttccct gtaccatgtc gcaccagact tttcagggtt
      181 ttgtgtcagc aaacagattg gcacgtccgg caaaagttct tttacagaat caatccacgc
      241 gccatacgtc gcccgatatg accgaccatg gtcatacccg tcgttgtgag aagtcgataa
      301 gaagcacaga acttgcccgt agttcggggt aagcttggga agccttgttg cgtccgcaag
      361 atagtttcca gccccggtga ttcctgcacc agaaacgctt ccgttaacaa ccgttagcgt
      421 cggagaacca gtgaagtacg cagaactgga tgatttcgga taccacagat ccggcataac
      481 cggagcaacg atttttccgt cctctccatc cctgatgata atctcataca gcctggtcac
      541 cctgactacc gccgatgtag acacaccgcc agcctcgtat cctatcgtag agttgttata
      601 aaccgagacc gcacctggag ttgtcaccgt tgatccgatt tgcgtccacg tttccccgtt
      661 tggcgattta tacgcacgaa aaacacggtt ccccgcccca tcgtccggcg tgaaaatcca
      721 tctcacccag taatcggtgt tgtctgttat tcctgtgttc gtagcagcca tcgttaccag
      781 cgcggtgccg tctgtggaat aggcgaaata cactatacca gtggagttta tatacccgta
      841 ccatcctcta tagccggggg ttccggattt tccgaaaacg tttacttgtg ctccgggatt
      901 gctccagctc gccaatcgca ttttcaccga gacatcaatc gtcccagaaa tatacggtga
      961 ctccgacgct gccagagttc tggtacttgt tccagtcgca ccgtcgaaat acctttcccc
     1021 gtctgggcct gtctgtatca cgaccggtga tgaatatttc tgcgttgtat cggagaacag
     1081 tatagttctg acagtccacg ccggattatc agaggcaagt tgatttgcga tcagacgtgg
     1141 ccactccccc gtatcgttcc ccgtagaatc cccgagcacc tgaatcgcca cagattgcga
     1201 ttggtctgat agcgcagcaa tcaacgccac agcagctctt tttcccgcat ccgcc
//
LOCUS       DAOQLC010000418         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_101211_length_1221_cov_14.913379, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000418 DAOQLC010000000
VERSION     DAOQLC010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101211_length_1221_cov_14.913379"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1201
                     /locus_tag="PL000_23135"
     CDS             113..1201
                     /locus_tag="PL000_23135"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91827.1"
                     /translation="MRRSTHKDILDFLQQVTPGASSGQMSRRFCLMASLIQACIRNGH
                     CRLESLSESTDAYAARKKSSLLQQAKRWLGNKWTDWQTFYLPFVQHFLLGLAAKGEIV
                     FVVDGSQTGSTNTTLMLSALCRGFAIPVAWVVRKGGKGHFPEEMHTDLLNMIAPLCPA
                     GCRIVLLGDGEFDGQALLKCCVDNHWSFVVRTSSDRLIDFGGEVARFDSMQTTRSVCF
                     IWGAVPLANAVYWRGKGHSKPLFLLTNLELGYEACRYYKRRFKIETLFKHLKSAGFHL
                     HKTRLQCSEKLANLIIVVAFSFVFSFCMGVFIKENEPLEKIELIVRKDRLANINPIVI
                     AQLAITHNCQCVATFFSDLPKNFNEIFT"
BASE COUNT          284 a          337 c          316 g          284 t
ORIGIN      
        1 ttttgaaagg gtacacattt tccggtgaac caactgggtt tagttctttg agaagtcgga
       61 aaagaaaaat ggaagggaaa tttgtgtttc tcacgcataa atctcgcctt ccatgcgccg
      121 aagcacccac aaagatatcc tcgatttttt gcaacaggtt acgcccggtg ccagtagcgg
      181 tcagatgagc agacgattct gcctgatggc cagcctgatc caggcctgca tacgcaacgg
      241 ccactgccgt ttggagagcc tgagcgaatc caccgatgcc tatgcggccc gcaagaaatc
      301 cagcctgctg caacaggcca agcgttggct gggcaacaaa tggacggact ggcagacctt
      361 ttacctgccc tttgtccagc actttttgct ggggctggcg gccaaaggag agatcgtgtt
      421 cgtcgttgac ggcagccaga cgggctcgac caacaccacc ctgatgcttt cagccttgtg
      481 caggggattt gccattccgg tggcctgggt cgtcagaaag ggaggaaagg ggcatttccc
      541 ggaggaaatg cacaccgatc tgctcaacat gatcgccccc ctttgtccgg cagggtgccg
      601 gatcgtgctt ttgggcgacg gggagtttga cggccaggcc cttctgaagt gctgcgtgga
      661 caatcactgg tcattcgtgg tcaggacgtc ctcggatcgc ctcatcgatt tcggcggcga
      721 ggtcgcccgc ttcgattcca tgcagaccac ccgcagtgtc tgcttcatct ggggcgcagt
      781 gccgctggca aatgcggtgt actggcgcgg aaaagggcat tcaaagcccc ttttcttgtt
      841 gaccaacctg gagttgggct acgaagcctg ccgatactac aaacgcaggt tcaagatcga
      901 aaccttgttc aagcacctta agtctgccgg atttcacctc cacaaaaccc gcttgcaatg
      961 ctccgaaaaa ctggcaaacc tgatcatcgt ggtcgctttc tccttcgtat tcagtttctg
     1021 catgggcgtg ttcatcaaag aaaacgaacc cctggaaaag atcgaactga tcgtgcgaaa
     1081 agacagactg gccaacataa accccattgt catcgctcaa cttgctatca cgcacaactg
     1141 ccagtgcgtc gctactttct tttccgactt gccaaagaac ttcaatgaga tttttacctg
     1201 aaaaatctgt accctttcaa a
//
LOCUS       DAOQLC010000419         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_101388_length_1220_cov_24.709871, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000419 DAOQLC010000000
VERSION     DAOQLC010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101388_length_1220_cov_24.709871"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PL000_23140"
     CDS             <1..432
                     /locus_tag="PL000_23140"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HNC91828.1"
                     /translation="HNFKERKMENLKEIVAPFYNECLTVNAETNVAEKMGQILADSFK
                     SKGSNDVKTKEQLIGQIQFFWKLVPDLKWEIQEMIQEGNQVVVRSLASGTPNGNFMGV
                     QTDGTKSFKTMTIDIHTVQNGQVAEVHHLEDWSTAIKQLQG"
     gene            509..979
                     /locus_tag="PL000_23145"
     CDS             509..979
                     /locus_tag="PL000_23145"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNC91829.1"
                     /translation="MQSASKKFSLTQFKLFLDILVLILFIAINIPGTTGIPNHEWISF
                     VFALPFLIHLTLNWKWIVSVFSRLFQRLPGETRFNQIWDSILFLLMIFVSFSGAVLSE
                     SVLPALGIHLEIDLFWRSLHTGMANLLMLMFGVHLTMHWKWIVTNFNRYVLQKA"
     gene            982..>1220
                     /locus_tag="PL000_23150"
     CDS             982..>1220
                     /locus_tag="PL000_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91830.1"
                     /translation="MNFVKEYILPALVILLVAATLMFGYQFLAQASWAEGIRASAAST
                     PPEIGPAPTNLTEFLTAFATSTVRMLILIGLPFAIT"
BASE COUNT          305 a          340 c          272 g          303 t
ORIGIN      
        1 cataacttca aggagagaaa aatggaaaac ctcaaggaaa tcgtagcccc cttttacaac
       61 gaatgcctga ccgtcaacgc ggagaccaat gttgcagaga agatgggtca aatcttggca
      121 gacagcttca agtcgaaggg ttcgaacgat gtcaaaacga aggaacaact catcgggcag
      181 attcaattct tctggaagct tgtccccgac ctgaaatggg aaatccagga aatgatccag
      241 gaaggcaatc aggtcgttgt acgcagcctt gcttctggca ccccgaacgg aaatttcatg
      301 ggtgtgcaga ccgatggaac caaatccttc aagaccatga cgattgacat tcacaccgtc
      361 cagaatggtc aggtcgccga agtccatcac ctcgaagact ggtcaaccgc catcaagcag
      421 ttgcagggat aggtcttcgg taattggaga ttggaggttg gtaattgatg actaatctcc
      481 aatctcaatt ttgccaaagg gaaacttcat gcaatccgcc agcaaaaaat tcagcctcac
      541 gcaattcaaa ctctttctcg atatccttgt gctcattctc ttcattgcca tcaatatccc
      601 aggcacaacg ggtatcccaa accacgagtg gatcagtttc gtctttgcac tgccattcct
      661 gattcacctc accctcaact ggaaatggat cgtcagtgtc ttctcgcggc tcttccaaag
      721 actgccaggt gaaacgcgct tcaaccagat ctgggattcg attctcttcc tgctgatgat
      781 ctttgtctca ttctctggag cggtactctc cgagagtgtt ctacccgcgc tcggcattca
      841 ccttgagatc gacttgttct ggcgctcatt gcacacaggc atggcaaacc tgctcatgct
      901 gatgttcggc gttcacctca ccatgcactg gaagtggatc gtcacaaact tcaaccgcta
      961 cgttttgcag aaggcttaac catgaacttc gtcaaagaat acattctccc cgcgttggtg
     1021 attttgctcg tcgccgccac actcatgttt ggatatcaat ttctcgcgca ggcttcatgg
     1081 gcagagggga ttcgcgcaag cgccgcctca accccgcccg aaattggtcc cgctccgacg
     1141 aacctcaccg agttcctgac cgcatttgcc acttccaccg ttcgtatgtt gattctcatc
     1201 gggctgccat tcgctattac
//
LOCUS       DAOQLC010000420         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_101389_length_1220_cov_23.270386, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000420 DAOQLC010000000
VERSION     DAOQLC010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101389_length_1220_cov_23.270386"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PL000_23155"
     CDS             complement(<1..419)
                     /locus_tag="PL000_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91831.1"
                     /translation="MSNSTSIDNSNLLAARRLLEMAKQADARRSGKTVKEKSALEYMQ
                     EDQALISAPAFDAQKQEAAALPQIENINVNLSDLRNVKPQTGGTATVSEEQSIQIEFE
                     LRYRSNTPLDGLLIHDRNYAESDRYLFKFADGATFTIL"
     gene            complement(594..>1220)
                     /locus_tag="PL000_23160"
     CDS             complement(594..>1220)
                     /locus_tag="PL000_23160"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91832.1"
                     /translation="KRGYTTKNNVKAEALVNSFESESTDTEELEKEVGTSSQTVLQEP
                     KIAPAPKIEIVEEAPAEQLPQTNANVTFEMEAAPQISTELAYACLLIPRFNDHYLTGD
                     ITKDLVKWVKDVCISYGWRLDAITIRPGYLQWIMTVPFTENPARFMKVLRQQTSQKIF
                     EDYPRFKRKNVSLDFWAPGFSVVHGSSPQSPEEINSFILQIRRQQGIY"
BASE COUNT          292 a          296 c          302 g          330 t
ORIGIN      
        1 aggatggtga atgttgcccc atctgcgaat ttgaaaagat agcggtctga ttcggcgtaa
       61 ttacggtcat ggatcaacaa tccatccaac ggtgtattcg aacggtaacg cagttcgaac
      121 tcgatctgga tgctttgttc ttcagaaacg gttgctgtgc cgcctgtctg cggtttgaca
      181 ttacgcagat cagacaggtt tacgttgatg ttttcaatct ggggcagggc ggcggcttcc
      241 tgtttttgcg catcgaaagc aggcgcactg atcaaagcct ggtcttcctg catgtattcc
      301 agtgcagatt tttccttcac ggtctttccg cttcggcgcg catctgcctg tttggccatc
      361 tccaacagcc gtctggctgc cagcaaattt gaattgtcaa tcgatgtgga attactcatg
      421 ccagcctccc gggatggtga tcacatatca agcatcgaca tgttcacgga aaacttgagg
      481 cttttcaaaa aattgatctg ttggatgaaa aaaacgcatc ccatcgacct gaaaccgggt
      541 gctgttaaaa ataaaaattt cccccgctgt aacatgctca acacaacaca ggtctaataa
      601 ataccctgtt gcctgcgtat ctgtaaaatg aaactattga tctcttccgg tgattgcggg
      661 gaacttccat gtaccactga aaaaccaggc gcccagaaat caagtgaaac attcttccgc
      721 ttgaagcggg gataatcctc gaatattttt tgagaagtct gctgacgcag taccttcatg
      781 aagcgggcgg gattttccgt aaaaggcacg gtcatgatcc actgaagata cccggggcgg
      841 atcgtgatcg catccagccg ccagccgtag gaaatgcaaa catccttgac ccacttcacc
      901 agatctttgg taatgtcccc ggtcaggtag tggtcattga agcgtggaat cagcaggcag
      961 gcatacgcca gctcagtgga aatttgaggg gcggcttcca tctcaaaggt cacattggcg
     1021 ttggtttgcg gaagttgttc ggcaggcgct tcttcgacga tctcgatttt tggtgcaggg
     1081 gcgatcttgg gctcctgcaa aaccgtttgc gaagaagtac cgacttcctt ttccagctct
     1141 tcagtatcag tggattcgga ctcaaatgag ttcacgagtg cctcggcttt gacgttattt
     1201 tttgtagtat acccccgctt
//
LOCUS       DAOQLC010000421         1207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_103575_length_1207_cov_31.750000, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000421 DAOQLC010000000
VERSION     DAOQLC010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103575_length_1207_cov_31.750000"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PL000_23165"
     CDS             <1..359
                     /locus_tag="PL000_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91833.1"
                     /translation="PRKGARVMDMVGVLQANGWAKEGRKWRKGDALADAATAYQSCFA
                     PQTPEYLGKVVRWYYSTQAPGPIVYASNGNTVSLSYGARPCMTLPDEFPDDVDYAWYI
                     GKAEAMLRDVGYYSLT"
     gene            386..703
                     /locus_tag="PL000_23170"
     CDS             386..703
                     /locus_tag="PL000_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91834.1"
                     /translation="MLTLQPSLMANMGHDHLIRSLECEPQIMRTPVELELMARCERLA
                     DELADRPTEAEMEKRYESPLEQSEFRAQLIKEIIELCDRPGSKKDLVSAIKMALENSY
                     VEL"
     gene            716..1048
                     /locus_tag="PL000_23175"
     CDS             716..1048
                     /locus_tag="PL000_23175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91835.1"
                     /translation="MKTILFILAAYAFVGWNDAHAGPNECLQGVRAATEAYRMVVYDA
                     TNPPRDALLHALDFRISQQPFPVDRQHARMAVAVIAVQLRDSGVRAWSGWQVDWIAEE
                     YIRRECVK"
BASE COUNT          293 a          303 c          363 g          248 t
ORIGIN      
        1 ggccgcgcaa gggcgccagg gtcatggata tggtcggcgt gttgcaggcc aacggctggg
       61 ccaaagaggg tcgaaagtgg cgcaagggtg acgccctggc ggacgcggcc acggcctacc
      121 aatcttgctt tgccccgcaa acccccgaat acttgggcaa ggtggtgcgc tggtactaca
      181 gcacccaggc gcccggccct atcgtctacg ccagcaatgg caatacggta agcctttcct
      241 atggtgcccg gccgtgcatg acgctaccgg acgaattccc ggacgacgtc gactatgcgt
      301 ggtacatcgg gaaagcggaa gccatgttgc gcgacgtggg ctactactcg ttgacataga
      361 taaattattg atctaggata cacccatgct gacattgcaa ccaagcctta tggcaaacat
      421 gggacacgac catttaatac ggtcgttgga gtgcgaaccg caaattatgc gaacgcccgt
      481 cgaattggaa ctcatggccc gttgtgaaag gctggcggat gaattggccg accgtccgac
      541 ggaagctgaa atggaaaagc ggtatgaaag tccgcttgag caaagcgaat ttcgggcgca
      601 attgattaaa gaaataatcg aactttgcga taggccagga agtaaaaaag acctagtttc
      661 cgccataaaa atggcgcttg aaaatagtta cgttgaactt taaggagccg caaccatgaa
      721 aactattttg ttcatcttgg ccgcctacgc cttcgtcggc tggaatgatg ctcatgctgg
      781 cccgaatgaa tgtttgcaag gcgtaagggc ggccacggaa gcctatcgta tggttgttta
      841 cgacgccaca aatccgcccc gtgacgcctt gttacatgcg cttgacttcc gaatcagtca
      901 acagccgttc ccggttgacc gccagcatgc ccgaatggcc gttgccgtca ttgcggtaca
      961 gttgcgcgac agtggcgtaa gggcgtggtc cggttggcaa gttgattgga ttgccgaaga
     1021 gtatatccgc cgggagtgcg tcaagtgact tgccagcacg tcgggagcgg gtgcaatgcc
     1081 ccggaagggg aatgcttggg attttgtcta aacaaggggt acgaagtgca atacaagaac
     1141 ggaacgaagc ttaccgaccg cgaatccggc gccgtgtttg tggtcagcgg gtcgaatgcg
     1201 gaggaaa
//
LOCUS       DAOQLC010000422         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_103733_length_1206_cov_17.929626, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000422 DAOQLC010000000
VERSION     DAOQLC010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103733_length_1206_cov_17.929626"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>1206
                     /locus_tag="PL000_23180"
     CDS             191..>1206
                     /locus_tag="PL000_23180"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HNC91836.1"
                     /translation="MNRFIKFFSIVALLAFIATTIPVRAANPTATLPGLSISGINIVA
                     NGQPIRLRGVNMGDPFWARDPNVYNYSLADYATLSQTWHANVVRISIFPTQWKNMDRP
                     TLMAGLASEINAALNNGLYVIISYHVIGWPDGYSQPAYPGNPVDTYDSSMSVATSFWA
                     QMAQTYGSDTRIIFDLWNEPVHDAMDYVGSDPNPYWAPLKAYYETLIQTVRSNGGQNI
                     VIATGNRWASWLVGIKDNPLSDSNVIYAYHKYSIEGLNTSTEWDKDMGGLLGVKPVIV
                     SEWGYEDVDVSNPTWPGSEASYGDPFTQWMDSHNLSSLAWMYHDDWTPALLKADGSLT
                     IYGA"
BASE COUNT          292 a          312 c          306 g          296 t
ORIGIN      
        1 gggccgagca ggcgtacaac gatggcttac tccctgcctg cggcacggat agcggcacag
       61 gcaaaccgat gttctgcccg agtgaattgg tgaatcgcgg ttggggtgcg tacctgatcg
      121 tcaaagccaa gaacttgccc acaccgtaat gcggttgact ggtcttccaa cagagcgttt
      181 aggagaaaca atgaaccgct ttataaagtt cttttcgatc gttgctctgc ttgctttcat
      241 cgcaacaacg attcctgtac gtgcagcaaa cccaacggca accttacccg gattatccat
      301 ctccggtata aatattgtgg caaatggaca gccgattcgg ttacgaggcg tgaatatggg
      361 tgaccccttc tgggcgcgtg acccaaatgt ttacaactac tcgctcgccg actatgccac
      421 attgtctcaa acctggcacg cgaatgtggt gcgcatcagt atttttccga cccaatggaa
      481 aaacatggat cgtcccaccc tgatggcggg tctggcaagt gaaatcaatg cggccctcaa
      541 caatggcctg tatgtgatca tttcctatca cgtcattggc tggcctgatg gatattccca
      601 gcctgcatat cccggtaatc cagtggatac ttatgattcc agcatgagtg tggcgacctc
      661 cttttgggct caaatggctc aaacttatgg atcagacacc cgtatcatct ttgacctttg
      721 gaatgaacct gttcacgatg ccatggatta cgttgggagt gacccaaacc catactgggc
      781 tccgcttaag gcttattacg agaccctcat tcaaacggtc cgcagtaatg gtgggcaaaa
      841 tatcgtcatc gccactggta atcgctgggc cagttggctg gtgggcatca aagacaatcc
      901 gctttcagat tcgaacgtca tctacgcata tcacaagtat tccatcgaag ggctgaatac
      961 ctccacagaa tgggataaag acatgggcgg gttgctcggc gtcaaacctg tcattgtcag
     1021 tgagtgggga tacgaagatg tggacgtatc caacccaacc tggcctggct ccgaggcatc
     1081 ctatggcgat ccgttcaccc agtggatgga cagccataat ttgagcagcc tggcttggat
     1141 gtatcatgat gattggacgc cagctttact taaagcggat ggcagcctca ccatatatgg
     1201 ggcgtt
//
LOCUS       DAOQLC010000423         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_105241_length_1197_cov_12.376532, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000423 DAOQLC010000000
VERSION     DAOQLC010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105241_length_1197_cov_12.376532"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..1107)
                     /locus_tag="PL000_23185"
     CDS             complement(55..1107)
                     /locus_tag="PL000_23185"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HNC91837.1"
                     /translation="MPNEPTLNLLAVFDERWKNFRTRYKACRAEFTDAAVHDLRVASR
                     RMLAVLGLVKVLAPHPRVKKVRQFVKQTIENLNELRDVQVMLTEVSTSVEGLPELRAF
                     QKFLMKREARLMQMAYKRIKQNRLAEPRKRIERIRASLETEQMEGGAFNQRLLEAAKQ
                     AHALAEQALGQVDPLHISSIHRLRLAFKKFRYTVEMVHPLIEGFPPERFKTMHDYQGR
                     MGDIQDAEVFLGALSRYVKKHALEDAMKNALQSRQLKLDSLVGVFLENKDELTVFWNF
                     AAEEENESVHHSSRHRSGGGGPKVRGRQPAPVDRKRRGQNEKDRAGLAETGSATGPDS
                     KQPSRADDSDSGNIGR"
BASE COUNT          263 a          327 c          264 g          343 t
ORIGIN      
        1 ccatcaagcg ccagatgttc tgtggctatc aacctgcttt ttttgacatt taatttacct
       61 gccaatattt ccgctgtcgc tatcgtccgc acggctgggc tgcttagaat caggtccagt
      121 tgcacttcca gtttcagcaa gccctgcgcg atctttttca ttttgtccgc gcctttttcg
      181 gtcaacgggc gctggctgtc ttccgcgtac tttgggtccc ccgcctccac tgcgatggcg
      241 tgacgaatga tgtacagatt cattttcttc ctccgctgca aaattccaaa atacggttaa
      301 ttcatccttg ttttcaagaa aaacacccac aagcgaatcc agtttaagtt ggcgcgactg
      361 aagtgcattt ttcatcgcat cttcgagtgc atgttttttt acataccttg aaagcgcacc
      421 cagaaatacc tccgcatcct gaatatcccc catcctccct tgataatcgt gcatggtttt
      481 gaaacgctca ggcggaaacc cttcgatcag tggatggacc atctccaccg tatagcgaaa
      541 ttttttgaac gccagcctca aacggtggat gctggaaatg tgcagcggat ccacctgacc
      601 gagagcctgc tcggctaaag catgggcctg tttcgccgct tcgagcagtc tctggttaaa
      661 tgccccgcct tccatttgct ctgtttcaag gcttgcccga atccgctcaa ttcttttcct
      721 gggttcagca agcctgtttt gtttgatgcg cttgtaagcc atctgcatta acctggcttc
      781 acgtttcatc aaaaacttct ggaacgcccg cagctcaggc agcccctcaa cggacgtgga
      841 tacctcggtc agcatcacct gcacatcacg gagttcattt aaattttcga tggtttgttt
      901 gacgaattgc cgcaccttct tcacccgcgg atgtggagcg agcaccttta caagccccaa
      961 cactgccagc atacggcggg atgccacgcg tagatcatgt acggctgcat cagtgaattc
     1021 cgcacggcag gccttgtaac gtgtgcggaa gtttttccag cgttcgtcga aaacagccaa
     1081 caggttcaag gtgggttcat tgggcatgat gatcgcctta catcttatca tttttgttca
     1141 tcaaatgtta actggctctt aagtcgaaaa taaaggcttt gaagtatgat ttcagta
//
LOCUS       DAOQLC010000424         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_105404_length_1196_cov_20.086766, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000424 DAOQLC010000000
VERSION     DAOQLC010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105404_length_1196_cov_20.086766"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1196)
                     /locus_tag="PL000_23190"
     CDS             complement(<1..>1196)
                     /locus_tag="PL000_23190"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC91838.1"
                     /translation="RLLTTIAASLGTALENARLFDETQRLLRETNTLAEVGRDISSSL
                     DLQTVFENIAAQAYNLLQGDLSALFIPEGDGIFRAIVAVGENAEELRNETIESGQGIL
                     GSIAKNKAAEIVNDTNNDPRALTITGTDTLPDEHLLAIPLLAKDELKGLMAVWRTGKN
                     KDFTTNEMDFLTNLSRQAVIALQNSQLFNEAQSARAAAEHANSAKSTFMANMSHELRT
                     PLNAIIGFTRIVRRKAEGILPEKQTENLDKVLSSSEHLLGLINTVLDIAKIEAGRVDV
                     VPADFDVLALADQCAFLATPLLKPGVTLTKQTADPFTAFSDQDKIKQIIINLLSNAAK
                     FTHNGNISLSVSHSDSTMNIAVQDSGIGISEEALGRVFEEFQQADTTTTRQYGGTGLG
                     LAISRNL"
BASE COUNT          222 a          269 c          392 g          313 t
ORIGIN      
        1 aggttgcggc tgatggcgag gccgagacct gtgccgccgt attgacgggt ggtggtggta
       61 tcggcttgtt ggaattcctc gaagacacgg ccaagagcct cttcactaat gccgattccg
      121 ctatcctgta cagcgatatt cattgttgag tcgctgtgcg atacgctgag agaaatgttg
      181 ccgttgtgtg tgaatttggc ggcattgctc aacaggttga tgatgatctg tttgatcttg
      241 tcttgatcgg agaaggcggt gaatgggtct gcagtttgct tggtgagtgt gacgccaggt
      301 tttagcaggg gggtggcaag gaaggcgcat tggtctgcca gggcaaggac atcgaagtcg
      361 gcggggacga catccacccg gcctgcttcg atcttggcga tatcaagcac ggtgttgatc
      421 aggcccagca agtgttcact gctggagagt accttatcca gattttcagt ttgtttttcg
      481 ggcaatatgc cttcggcttt tctgcggaca atgcgtgtga agccgatgat ggcattgagc
      541 ggggtgcgca gttcgtggct catgttggcc atgaaggtac tcttggctga gttggcgtgt
      601 tccgctgcgg cacgggcaga ctgtgcttcg ttaaagagtt gcgagttttg cagggcaatg
      661 acagcctgcc gtgagaggtt ggtgaggaaa tccatctcgt tcgtggtgaa gtctttgttt
      721 ttgcctgtac gccagaccgc catgaggcct ttgagttcgt ccttggccag caaagggatg
      781 gccagcagat gctcatcggg cagggtgtct gttccggtga tggtgagcgc gcgcgggtcg
      841 ttattggtat cgttgacgat ctcggcggct ttgtttttgg caatgctgcc taatatgcct
      901 tgccccgact cgatggtttc attgcgtaat tcttcggcat tttcacctac agccacaatg
      961 gcgcggaaga taccgtcgcc ttcggggatg aaaagcgcgc tcaaatcacc ctgaagcagg
     1021 ttgtatgcct gggcggcaat attctcgaag acggtttgca ggtccaggct ggaggaaata
     1081 tcacgaccaa cttcggcgag ggtgttggtt tcacgtaaga ggcgctgggt ctcgtcgaag
     1141 agacgcgcat tctcgagcgc ggttcccagc gaggcggcga tggtggtcag caggcg
//
LOCUS       DAOQLC010000425         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_106511_length_1190_cov_16.771806, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000425 DAOQLC010000000
VERSION     DAOQLC010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106511_length_1190_cov_16.771806"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..853)
                     /locus_tag="PL000_23195"
     CDS             complement(68..853)
                     /locus_tag="PL000_23195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91839.1"
                     /translation="MSIIRKGYVDTPHGQIHFRQLNQVDGIPLVLLHQTASSGMMFER
                     MMTLLSDSFHTLALDTPGFGASFSPPSLFTTHYLSSTLHAALTNLGVESCYLFGHHSG
                     SAIAVQMAHDHPAFVKKMVLSGAPLLSDSQINGLKASLKPFAIAEDGSHLTQVWERIR
                     KRDLTLPLETVHREVLLTQSAREAAQSTFQAVFEQPFAEQLSALEIPILVMAGEHDTL
                     RASLEPAFSVLKNGTMKMIPNEGPYICDQNPQAVADVIREFIL"
     gene            971..>1190
                     /locus_tag="PL000_23200"
     CDS             971..>1190
                     /locus_tag="PL000_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91840.1"
                     /translation="MTDQLGYRKKFGVIIPSTNTIVEPEFYRMTVPGVTPHVSRIHIR
                     DQKLDSDEAFVRLLEQIRVEIQYAVERVM"
BASE COUNT          315 a          297 c          303 g          275 t
ORIGIN      
        1 gcgagcccga caatgacgaa gaagacattt tgcataatgg ttgctcctca aatttacggg
       61 gtcaaagtta taaaataaat tccctgatca catctgccac cgcttgcgga ttttgatcac
      121 agatgtaagg accttcgtta ggtatcatct tcatggtgcc gttcttcaac acggaaaaag
      181 caggctccaa acttgcccgc agcgtgtcgt gctcccctgc catcaccaag atgggaatct
      241 caagcgcgga taactgctcc gcgaacggtt gctcgaacac ggcttgaaat gtgctttgcg
      301 ccgcctctct cgcggactgg gtgaggagga cttctctatg aacagtttcc aacgggagag
      361 ttaagtctcg cttccgaatc cgctcccaga cttgggtgag atgcgagccg tcttctgcga
      421 tggcaaatgg tttgaggctg gctttcaatc cattgatctg cgaatcgctt aacaacggcg
      481 ctcctgacaa gaccatcttt ttcacgaagg cgggatggtc atgtgccatc tgcacagcaa
      541 tggctgaacc agagtgatga ccgaacaaat aacaagactc cacgccgagg ttggtcagag
      601 cggcatggag tgtggatgaa agataatggg tagtgaacag gctgggggga gaaaaagagg
      661 cgccgaatcc tggggtgtct aatgccagag tgtggaacga gtctgagagc agagtcatca
      721 tccgctcgaa catcatcccc gaactggcag tttgatggag cagcacaagc ggaattccgt
      781 caacctgatt gagttggcga aaatggatct gaccgtgcgg cgtatcgaca tagcctttgc
      841 ggataattga catctctctc cagaatgtta taatgttata acatttagag ttgttagtca
      901 agttaaaaat gaccgcggac aaatacgcca gacctctgat tcatttttca tcccaaagga
      961 gacctccccc atgacagacc aactcggcta cagaaaaaaa ttcggtgtca tcattcccag
     1021 cacgaacacc atcgtggaac ctgagttcta ccgcatgacc gtgccaggcg ttacgccgca
     1081 tgtctcgcgc attcacatcc gcgaccagaa actcgacagc gatgaagcct ttgtacgttt
     1141 gcttgaacaa attcgcgtgg agattcaata cgcagtcgaa cgcgtgatga
//
LOCUS       DAOQLC010000426         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_107202_length_1186_cov_25.961981, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000426 DAOQLC010000000
VERSION     DAOQLC010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107202_length_1186_cov_25.961981"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..785
                     /locus_tag="PL000_23205"
     CDS             366..785
                     /locus_tag="PL000_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91841.1"
                     /translation="MERKEAAAAGLKKYNTGRPCKHGHIADRYVDSGACSACLKNMVA
                     VKYAGGAESRQVIADRTKQIYLFVRSAGLPAIKMMVDSLVAARLPSIAPDAVNPEPYW
                     SKQVSRDTFQIKVRVPVEDVDTAYTMGKLLLEPEIKK"
     gene            782..1024
                     /locus_tag="PL000_23210"
     CDS             782..1024
                     /locus_tag="PL000_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91842.1"
                     /translation="MTNSTFVAAQDLPQVFAGYSFRLSDMKVILTDGTALRRSQFKVL
                     FGGLTFALDRNNEKITRDAWRAFIDNAAFRPPIVYK"
BASE COUNT          284 a          279 c          320 g          303 t
ORIGIN      
        1 gcgttgtttg ggcgtcaggc tcatagtgtc ctaattctag ccccaggcgg ccacaggaat
       61 caaccacaat gcggggtcgc tgtcagcatt gccccgctat ttttacgggg tacgactcgg
      121 ggtggtaacg taaccccttg attttattac tctttatctc tctttacccc tctacccctt
      181 aaaaatagaa agctgtatac atagacgccc aatatagact atatatatct atatctaaat
      241 aacgcactgt ataccgtcgg gttttggatg tacggggagg taggggtcgg ggtacgattt
      301 ttcataaaaa acgcttgatt tttacagtaa aatatagata attgagactt ttaggaggta
      361 aactcatgga acggaaagaa gcggcggcgg ctggtcttaa gaagtacaac actggccgac
      421 cgtgcaagca tgggcacata gccgaccgct acgtggattc cggcgcttgt tcggcatgtc
      481 ttaagaacat ggtcgccgtt aaatatgcag gaggtgccga atcccgacaa gtgattgcgg
      541 accgcacgaa gcaaatttat ctatttgtga ggtcggcagg attgccagca attaagatga
      601 tggtcgattc tttggtcgcg gccaggttgc ccagcatcgc gccggacgcg gtgaatcctg
      661 aaccgtattg gtccaagcaa gtgtcgcggg ataccttcca aattaaagtg cgtgtgccag
      721 tagaagacgt cgacacggcc tacaccatgg gtaaattgtt gcttgagccg gagattaaga
      781 aatgaccaat tcgacctttg tggccgctca ggacttgccc caagttttcg ccggctacag
      841 ttttcggcta tccgatatga aagtgatttt gacggacggt acagcgttgc gacgctctca
      901 atttaaagtg ctttttggtg gtttgacgtt cgcacttgac cggaataacg aaaagatcac
      961 gcgggacgct tggcgggcat ttattgacaa tgcggccttt cggccgccca tcgtttacaa
     1021 atgagtgtcg aagccggttt gctcggtgaa gagggccgcc aggcggtccc catagatacg
     1081 ctcaaattcg ccccgttcgg tcacatttac gccgcggatg ctgtagaagg tcacgcggta
     1141 caggtcgtcg accaggcgga tgcaaacctt gttggccttg ttcttg
//
LOCUS       DAOQLC010000427         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_107203_length_1186_cov_23.482759, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000427 DAOQLC010000000
VERSION     DAOQLC010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107203_length_1186_cov_23.482759"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PL000_23215"
     CDS             complement(<1..551)
                     /locus_tag="PL000_23215"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNC91843.1"
                     /translation="MGPLAVKTYPGEEWKLGPNRATFEPDYGRRGKLWTHGAFEPATG
                     QAAILMSPGRDSASHIQLLEKIFVEFPADRWLIIEDNLSIHTARQVKLALAAWPEVQV
                     LFIPKYACWLNLIEPWWKQLRSLALKGRRFETLDELTSTLNQALAYWNAHRHPYTWKK
                     KPQQQVTLFGGYSVCKGTVTSST"
     gene            complement(590..1126)
                     /locus_tag="PL000_23220"
     CDS             complement(590..1126)
                     /locus_tag="PL000_23220"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC91844.1"
                     /translation="MRTPVKLRTLTAEEVTEIKRLASSRKEPVRLVQRARIIAYMLED
                     SNLYASEAGQKAGYASVVTGPEWVRRFNEQGLAGLEDQPRPGRQPTHGQEVHSQLISL
                     ATQKPSTLGYPFTLWTLERLQSAFQERHDIHLSDSTIWEWLAAEGLEWKRQESWFHEA
                     EKHDPEFVEKRGASSRPT"
BASE COUNT          265 a          315 c          289 g          317 t
ORIGIN      
        1 taagttgatg aagtaacagt acctttacaa acgctataac cgccgaacaa agtcacttgc
       61 tgttgaggct tcttcttcca agtataggga tgacgatgag cattccaata tgctaaggct
      121 tgattgagag tggacgtcaa ttcatccagc gtttcaaaac gtctgccttt gagtgccaga
      181 gaacgcaact gtttccacca aggctcaatg agattgagcc agcaggcata cttgggaatg
      241 aacaagactt gcacttcggg ccaggctgcc agagccagtt tcacttgtcg agcagtgtga
      301 atactgaggt tgtcttcgat gatcagccag cgatcggctg ggaactcgac gaagatcttc
      361 tccaacaact gaatatgact ggcactatca cgccccggac tcatcagaat ggcagcctgt
      421 ccagtggcag gttcaaaggc tccatgtgtc cacagcttgc cgcggcgacc atagtctggt
      481 tcaaaggttg cccgattggg tcccaatttc cattcttcgc ccgggtaagt cttgaccgcc
      541 agcggtccca tctcatccac gcaaatcacc cgtgttcgtg ggatccgcat caggtaggcc
      601 gtgacgatgc tccccttttt tccacgaact ctggatcgtg tttctctgct tcatggaacc
      661 agctttcttg gcgtttccac tccagcccct cggctgccaa ccattcccaa atggttgaat
      721 ccgaaagatg gatatcgtgt cgttcttgaa aagcagactg cagacgttcc aaggtccaga
      781 gtgtaaatgg atatcccaag gtacttggtt tctgggttgc cagactgatc aactggctgt
      841 gaacttcttg tccatgcgta ggctggcgtc ctggacgtgg ttgatcttcc aaccctgcca
      901 gcccttgttc attgaaccga cgaacccatt ctggtcccgt aacaacactg gcatagcctg
      961 ccttctgccc ggcttctgaa gcgtacaggt tactgtcttc cagcatgtac gcaataatcc
     1021 tggctcgttg taccaaacga acaggttctt tgcgggatga ggctaatcgc ttgatttctg
     1081 tgacttcttc ggctgttaag gtgcgtagtt tgacgggtgt gcgcattttg cctcctggtt
     1141 tgccagaagg attttatctc actctcttac cgagagaacc acttag
//
LOCUS       DAOQLC010000428         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_107205_length_1186_cov_14.930150, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000428 DAOQLC010000000
VERSION     DAOQLC010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107205_length_1186_cov_14.930150"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..883)
                     /locus_tag="PL000_23225"
     CDS             complement(323..883)
                     /locus_tag="PL000_23225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNC91845.1"
                     /translation="MKKFSPLLLAVVLQAGGAQAATVTIDFEAYADNTLITTQYSPLG
                     VTFANASISHYHCCGTPSQTLVNSSNGGGDRLSPLIISFASPVSDVSYIYDNYGISGV
                     TGFASAFDSSHTLLQTIASPDNNYGNDLVSFSASGISSIEISNPFDGWLFAIDDLKFD
                     VPEPGSLALLGLALVGIAGSRKCKAT"
     gene            complement(924..>1186)
                     /locus_tag="PL000_23230"
     CDS             complement(924..>1186)
                     /locus_tag="PL000_23230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91846.1"
                     /translation="ESSGKIAFVMPHHEKSAGAPRKLPVSVILFRPSLTVTAHHTDVG
                     ISYITVIKIHRHQNLPTVITSIIDIDLCWHAYCFRDAGNLAY"
BASE COUNT          307 a          289 c          342 g          248 t
ORIGIN      
        1 catctggctc acgcctctaa gtagaatgac gatatgctaa gttgcgagga ttgggggcaa
       61 agcccggagg caaccctccg ggccagcgcc ttgatcctga tgggtgactg gtgcggaccg
      121 gttccgacac cacatgaatg aaaacgaggg cagcggacgt tgggccgaag ccagacgagg
      181 ggagatacaa aacaagttgc gcctacggcg cgaaaagttt tgttcgccct attgaccggg
      241 gcgggctaat gggtgacccc tcgcaccacg cagaagacaa aaaaccccgg cacaagaccg
      301 gggcgagatc tgcgaaaaca actcaagtgg ccttgcactt gcgtgagccg gcgatcccga
      361 ccaaggccaa gcccagaaga gccaacgatc ctggttccgg aacgtcgaac ttcagatcgt
      421 cgatggcgaa caaccagcca tcgaacgggt tgctgatctc gatcgacgaa atgccggatg
      481 ccgagaacga aacgagatcg ttcccataat tattgtccgg cgatgcaatc gtttgcagaa
      541 gggtgtggct gctatcaaaa gcgctggcga aacctgttac gccggagatc ccatagttgt
      601 cgtaaatgta cgaaacgtca gatacgggcg acgcaaagct gatgatcagc gggctaagac
      661 ggtcgccgcc gccattcgat gaattgacca gggtctgcga cggcgtgcca cagcaatggt
      721 agtgactgat cgacgcgttg gcaaaggtca cgcctagcgg cgaatattga gtcgtgatga
      781 gagtgttgtc tgcgtaagct tcaaagtcga tggtgacagt tgcagcctga gcaccccctg
      841 cctgaagaac aacggcgagc agcagcggcg aaaatttttt catggtgtgg attcctcgga
      901 atactgaatt cgtttgatta gtttcaatag gcaagattgc cagcatcccg aaagcaataa
      961 gcatgccaac aaaggtcgat atcaattata cttgtaataa ccgtgggtaa gttttggtgt
     1021 cggtgaatct ttattaccgt gatgtaagaa attccgacat ctgtatggtg ggctgtaacc
     1081 gttaaggaag gtctgaacaa gatgaccgaa actgggagtt ttctcggcgc accggccgat
     1141 ttttcatgat gcggcatcac gaatgcgatt tttccgctgc tttccg
//
LOCUS       DAOQLC010000429         1184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_107561_length_1184_cov_20.085031, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000429 DAOQLC010000000
VERSION     DAOQLC010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107561_length_1184_cov_20.085031"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1110)
                     /locus_tag="PL000_23235"
     CDS             complement(130..1110)
                     /locus_tag="PL000_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019437692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HNC91847.1"
                     /translation="MKQITFADAEYSNKRKQTRKELFLKEMEQVVPWKGLIGLIEPHY
                     PHGEGGRPSYPLMAMLRVHLLQNWFGYSDPAMEEALYETTILRQFAGLDLDRVPDETT
                     ILNFRRLLEQHELAAKILVVINGYLGDRGLSLRQGTIVDATLIHAPSSTKNKEGKRDP
                     AMHQTRKGNQYYFGMKAHIGVDEKSGLVHSVVGTAANVADVTQVGKLLHGEESTVSAD
                     AGYTGVEKRAEHVDRKVIWQIAARRGCYKKHQKNSFLYQTLRLIEHAKARIRAKVEHP
                     FRVIKRQFGYTKVRFRGLAKNTAQLITLFALSNLWMARRFLLANTGEVRP"
BASE COUNT          267 a          323 c          285 g          309 t
ORIGIN      
        1 agtgatattt cttagattct tcagatattc tcattgtaaa aaaggccgag atgcgcagag
       61 gacaatcaaa ctcgccgaaa atctcatgtt tttgtgacag cagagttctt tcagcaatca
      121 ttttgtatgt tacgggcgca cctctcctgt atttgccagc aaaaacctgc gagccatcca
      181 cagattggaa agagcaaaca gcgtgattag ctgtgccgta ttcttggcca atccacgaaa
      241 gcgcactttg gtgtagccaa actgccgctt gattactcgg aagggatgtt caaccttggc
      301 gcgaatcctc gctttggcat gctcaatcag gcgcagagtt tgatacaaga agctgttttt
      361 ctgatgcttc ttataacagc cgcggcgtgc agcaatctgc cagatgacct tgcggtcaac
      421 atgttcggca cgcttttcaa ctcctgtata gcccgcatcg gcactgacgg tgctttcctc
      481 gccgtgcagt agtttgccga cctgggtaac gtcagcgaca ttggcggccg taccgacgac
      541 actgtgcacc aagcccgatt tctcatcgac accaatgtgt gccttcattc caaagtagta
      601 ctgattgccc ttcctggtct ggtgcattgc cggatcacgc ttgccttcct tgttcttggt
      661 tgagctgggc gcatggatca gggtggcgtc aacgatcgtg ccctgacgca gcgacagacc
      721 gcgatctccc agataaccgt tgatgacaac cagaatcttc gccgccagct catgttgctc
      781 caacaaccgc cggaagttga gtatggtggt ttcatccgga acccgatcca gatcaaggcc
      841 ggcaaactgg cgcaaaatcg tagtctcata cagcgcttcc tccatggccg gatcactgta
      901 accaaaccag ttctgcaaca aatgcactcg cagcattgcc atcaagggat agctggggcg
      961 gccgccttct ccatgcggat aatgcggttc aatcaacccg atcaatccct tccacggtac
     1021 tacctgttcc atctccttca ggaataattc cttgcgggtc tgcttgcgtt tgttcgaata
     1081 ctcagcgtcg gcaaaggtta tctgtttcat gggttggctc tgaagggggg agatacctgt
     1141 attttgccag atcaatcacc gttcggcact ttttcagagt ttcc
//
LOCUS       DAOQLC010000430         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_108446_length_1179_cov_15.610320, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000430 DAOQLC010000000
VERSION     DAOQLC010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108446_length_1179_cov_15.610320"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PL000_23240"
     CDS             <1..380
                     /locus_tag="PL000_23240"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC91848.1"
                     /translation="MIRGLQSGARGYLLKDSSRESLLDAIHAAAKGETLLKPEILARV
                     LTSSSRGKPESTPQTDTTLTERELEVLQLAAKGERNKEMAYKLGITERTVKAHLQSIY
                     QKFGVDSRAAAVAVAAGKGLLEN"
     gene            828..>1179
                     /locus_tag="PL000_23245"
     CDS             828..>1179
                     /locus_tag="PL000_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91849.1"
                     /translation="MTLAIPGGMAQFFNELESALQDGTIDDVKHKEISQKYGIDWNKW
                     KIPIKSCSPAPPHNVPQWGMDHKRDVTIYAILDIPLNRETRPYGLPKFPIAPITNIDS
                     LLPNNAATDASDTHP"
BASE COUNT          334 a          271 c          284 g          290 t
ORIGIN      
        1 tgatgatccg cggtttgcaa tctggcgcgc gcggatattt gctcaaagat tcaagccgcg
       61 aaagtttatt ggatgcgatc cacgctgcgg cgaaagggga gacgttattg aagccggaga
      121 tccttgcgcg cgtgcttacg tcttcttctc ggggcaagcc cgagtctacg ccccaaaccg
      181 ataccacgct gacagaacgc gagcttgaag ttttacaact cgcggcgaaa ggcgaacgta
      241 acaaagaaat ggcgtacaaa ctgggaatca ccgaacgaac tgtgaaggct catctgcaaa
      301 gtatctatca aaaatttggg gtggattcga gagcggcggc ggtcgctgtg gcggcgggga
      361 agggattgtt ggagaattaa aagtgatagt caccttaaag gtgactgtca cttctttaaa
      421 ctttacttag caaaccgaaa taatagagga tttgtctttt gtcagtgggg ggcactgtaa
      481 gcgatgaagg cactgcctca tccaatacga gagcgattgc cgcctccatc tcgtcatggc
      541 gcgggttctt acaagaattt cgtcaatgaa tttcctgtgc gtaagtcaga caatggaacg
      601 aatcttttgg cgcaagttgt ctgtccagac gattaacatc gcagtgcgga taaggtcttc
      661 ttgttttaag aacagacagg cactatttgg gtgtttttca caggacgcat tcataaagca
      721 attcttttgt ctcctccata tcaccgtgat gaagaagcgt aatcgcgcat ctactaaaat
      781 agattccgca tgcgctacac aatccgcaga aagaacgcct caaaattttg acactggcaa
      841 tcccaggcgg catggcgcaa ttcttcaatg aactcgaatc cgccttgcag gatggcacga
      901 tagacgatgt gaagcacaag gaaatctccc aaaagtatgg aattgattgg aataagtgga
      961 aaattccaat taaatcttgt agtcctgcac cacctcataa tgttccacag tggggaatgg
     1021 atcataaaag ggatgtaaca atttacgcca ttcttgatat tccgctgaac cgcgaaaccc
     1081 gaccgtatgg gcttccaaag tttcccattg caccaataac aaatatcgat tctctacttc
     1141 caaacaatgc tgcaactgat gccagtgata cccatccat
//
LOCUS       DAOQLC010000431         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_111426_length_1163_cov_22.404332, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000431 DAOQLC010000000
VERSION     DAOQLC010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111426_length_1163_cov_22.404332"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PL000_23250"
     CDS             complement(<1..695)
                     /locus_tag="PL000_23250"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC91850.1"
                     /translation="MKICIVGGTGNISQPIVKLLLEKGHEVVLFNRGQTAKAPEGVRI
                     ITGDRHNREDFERKMQAEKFDAAIDMICFTAEDAASSVRAFRGVGWFVQTSTVCTYGI
                     QYDYLPVDETHPLRPNTEYGRNKAAADHVYLEAYHREKFPVVIIKPSTTYGPVQGMLR
                     QICWDFSWIDRVKKGKPVIICGDGFAIHQHLHVDDIAHAFVGVIGKEHTIGQIYNAVN
                     RGYYTWLDYHKLA"
     gene            complement(732..>1163)
                     /locus_tag="PL000_23255"
     CDS             complement(732..>1163)
                     /locus_tag="PL000_23255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91851.1"
                     /translation="RDLLKYIRLLKPDFVFTCDPWLTYEGHRDHIQTGLATAEAVMFA
                     GLTKIASSDPKVDSAYEPHDINGIAFYFTREPNVISDISSTWEEKVGALRCYEAQFTP
                     QDMEQLLAAMDAKSQQVAQGQGFMRGEALKVLNTSALHVGI"
BASE COUNT          250 a          298 c          319 g          296 t
ORIGIN      
        1 ttggcgagtt tgtgataatc gagccaggtg tagtatccgc gattgacagc gttataaatc
       61 tgcccaatgg tgtgctcttt gccaatcaca cccacaaagg catgtgcgat gtcgtccaca
      121 tgcaggtgct gatgaatcgc aaaaccgtca ccgcaaatga tgacaggttt gcccttcttc
      181 acacggtcaa tccatgagaa atcccagcaa atctggcgga gcattccctg cacaggtcca
      241 taggttgttg atggcttgat gatgaccacg gggaattttt cgcggtgata cgcttcaaga
      301 taaacatggt cagccgctgc cttgttccgt ccgtattcgg tattggggcg caggggatgc
      361 gtttcgtcca cggggagata atcgtattga atgccgtagg tgcaaaccgt ggatgtctgc
      421 acgaaccagc ccacgccgcg aaaagcacgg acgcttgaag cggcgtcttc tgcggtgaag
      481 cagatcatgt caatggctgc gtcgaatttc tcggcttgca tcttgcgttc aaagtcttcg
      541 cggttatgac ggtcgcccgt gatgatgcgc acaccttcgg gcgctttcgc cgtctgcccg
      601 cgattgaaga gcacgacttc atgtcccttt tcaagcagaa gttttacgat gggctggctg
      661 atgttgcccg tgccgccgac gatacagatt ttcataggtt cctctcgaag tgatgtgagt
      721 tgcgtaaccc gttatattcc gacatgcaaa gcactggtat tcaacacctt caacgcctca
      781 ccacgcataa aaccttgtcc ctgcgccact tgctgtgact tcgcatccat ggctgcgagc
      841 aattgctcca tgtcttgcgg ggtgaattga gcttcgtaac aacgcaaagc gccgactttc
      901 tcctcccatg tggaggaaat gtctgagatg acgtttggtt cgcgggtgaa gtaaaacgct
      961 atcccgttga tgtcatgcgg ctcgtaagca gaatcaactt tcgggtcgga cgacgcgatt
     1021 tttgtcaatc cagcgaacat gaccgcctct gctgttgcca atcctgtttg gatgtggtcg
     1081 cggtgtcctt cgtaggtcag ccacgggtcg caggtgaaga cgaagtcggg cttgagcagg
     1141 cggatgtatt tgagcaagtc gcg
//
LOCUS       DAOQLC010000432         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_111987_length_1160_cov_28.236199, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000432 DAOQLC010000000
VERSION     DAOQLC010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111987_length_1160_cov_28.236199"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1160)
                     /locus_tag="PL000_23260"
     CDS             complement(<1..>1160)
                     /locus_tag="PL000_23260"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNC91852.1"
                     /translation="IADNVGDNVGDCAGMAADLFETYAVTIIATMLLGGLMITGAASA
                     NAVIYPLVLGGVSIIASIVGSFFVKAREGGKIMNALYRGLAVAGAIALVAFYPITTMM
                     FPEGIQIGDANVSAMNIYLASLIGLVLTAAMVVITEYYTATEYSPVRHIAQASTTGHG
                     TNIIAGLGVSMKATAAPVIAVCLAIWAAYDLGGLYGIAIAATSMLSMTGIIVALDAYG
                     PITDNAGGIAEMAELPDEIRNITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTH
                     ALSAGGQHLAFDLSDHMVIIGLFIGGMVPYLFGAMGMEAVGRAAGSVVVEVRRQFKSI
                     PGIMEGTGKPEYGTCVDMLTKAAIKEMMIPSLLPVLVPVLVGLILGPKALGGV"
BASE COUNT          193 a          350 c          431 g          186 t
ORIGIN      
        1 acgccgccca gggccttggg acccaggatc aggcccacca gcacggggac cagcacgggc
       61 agcagggagg ggatcatcat ctccttgatg gcggccttgg tcagcatgtc cacgcaggtg
      121 ccgtattccg gcttgccggt gccttccatg atgccgggga tggacttgaa ctggcggcgc
      181 acttccacca ccacggagcc ggcggcgcgg ccgacggctt ccatgcccat ggcgccgaac
      241 aggtagggca ccatgccgcc gatgaacagg ccgatgatga ccatgtggtc ggacaggtcg
      301 aaggccaggt gctggccgcc ggcgctcagg gcgtgggtgt agtcggcgaa cagcaccaga
      361 gccgccaggc cggcggagcc gatggcgtag cccttggtca cggccttggt ggtgttgccc
      421 acggcgtcca ggggatcggt gatgttgcgg atctcgtccg gcagttccgc catctcggcg
      481 atgccgccgg cgttgtcggt gatggggccg taggcgtcca gggccacgat gatgccggtc
      541 atggacagca tggaggtggc ggcgatggcg ataccgtaca ggccgcccag gtcataggcg
      601 gcccagatgg ccaggcagac ggcgatcacc ggtgcggcgg tggccttcat ggagaccccc
      661 aggccggcga tgatgttggt gccgtggccg gtggtggaag cctgggcgat atgccgcacc
      721 gggctgtact cggtggccgt gtagtactcg gtgatcacca ccatggccgc cgtcagcacc
      781 aggccgatga gggaggccag gtagatgttc atggcggaga cgttggcgtc gccgatctgg
      841 atgccttccg ggaacatcat ggtggtgatg gggtagaagg ccaccagggc gatggcgccg
      901 gccacggcca ggccgcgata cagggcgttc atgatcttgc cgccttcccg ggccttcacg
      961 aagaaggaac cgacgatgga ggcgatgatg gagacgccgc ccagcaccag gggatagatc
     1021 accgcgttgg cgctggccgc gccggtgatc atcaggccgc ccagcagcat ggtggcgatg
     1081 atggtcacgg cgtaggtctc gaacaggtcg gcggccatgc cggcgcagtc gcccacgttg
     1141 tcgcccacgt tgtcggcgat
//
LOCUS       DAOQLC010000433         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_112553_length_1157_cov_30.589837, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000433 DAOQLC010000000
VERSION     DAOQLC010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112553_length_1157_cov_30.589837"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1157)
                     /locus_tag="PL000_23265"
                     /pseudo
     CDS             complement(1..1157)
                     /locus_tag="PL000_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807917.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          232 a          315 c          306 g          304 t
ORIGIN      
        1 cggcctgacg acgttttagc cagcgccgct gcggcattgt cagtgacttg tacttgccac
       61 gatggatgat gttcacgtcc gggttgtcgg catccacgcc ccgaaatccg agatcgacaa
      121 tgacctgctt cggcgttgat cctgagtttt cgagtaaaat ctgtgtctgt gtgagttgct
      181 tatgaaggat atggccatcg tagggattgc cggggaaagt ttttgcgccg accatcaggc
      241 cgttcttgtg cgtgatcgca atgctggctt taacgccaaa ttcgtaaggc ttgcgcgctt
      301 tgcccttgcc gatacattcg acttccgggg catgcagtgc gtagagtttg ttcttgtctt
      361 tcggttgctg cttgtgaatc tgctgtgctc gctccagcag tgtgttcaga tgttggaacg
      421 atgcgggaca cgccagttct gccgatgcca gttttcgctt gacctctcgc agcacgatac
      481 caaggatcgt gcgttggcgt ttaacggttt tgcgcatgcg gcgaaattgc ttggcgtggg
      541 cataaccact agctttgcgg cgcaattcct tgccttcctt ggcgtaactc tgcttcaagg
      601 tgatgccgct tcgtttagcc acctgtacga ttttgctgcg cgcaatttcc agcaagcgaa
      661 tatccaccgg atgcgcgatc gctttttcct gtactgtcgt atcgacgatg acacgttcga
      721 attcagccgg tttgactacc ttcgcttgca cagcagcgtc aatggttgct ttcagcagct
      781 tttcgacacc ggattcacca atcgcagtgc ggaagcgacc tatctgagta gcgtcacagg
      841 gcagcctcgg cgtgtagtac acctgcccac taaaatactg ccagaccaca ttctcggacc
      901 agcgaatcgc cagttcttca tcactcagat tgaatgcgtg cttcagatac agcaaggaag
      961 ccatcaaccg aatgggcaaa cgaggccgcc ccgccagcaa tcaccaacgt cgtgtcgaac
     1021 agatcatcaa tctccaccac ttggctttga cgattcttgc gctcgaatgc tggtgcaagc
     1081 gctgcttcaa tatcgggcca cggtagtcga tttgccaata ccgccaaggg atggtgcaga
     1141 tcgatcatct ggtcgag
//
LOCUS       DAOQLC010000434         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_112745_length_1156_cov_22.037239, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000434 DAOQLC010000000
VERSION     DAOQLC010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112745_length_1156_cov_22.037239"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PL000_23270"
     CDS             <1..287
                     /locus_tag="PL000_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC91853.1"
                     /translation="YMPHEEKTARVGTWVFPREIIGSTPWLKTLWDQREKIQHIPTAI
                     VWGDSDIAFKEGELKVWTSLMKNHTLNVLHRIGHYPPEEAPEEVIKALKA"
     gene            284..988
                     /locus_tag="PL000_23275"
     CDS             284..988
                     /locus_tag="PL000_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91854.1"
                     /translation="MNQTIEKAIDAYGGREFWLKAKRIEAEVSTHGLAFTIKSRPTFK
                     RAKISMEIARPFSKLMPIGKITQLAGVLDEHHVRLEDSTGRIVEERKDARKYFPGGLQ
                     RLFHWDDLDMAYFANYAFWNYFTLPVLLMREDIDWKEIGPGWLEATFPESIPTHSKIQ
                     RFRFDLKTGLLIQHDYTVDIMSKLATAANVVLEHKQGAGFLYPSRRLVTPRSPSGKPL
                     PAPTLIDITVHEFQVK"
BASE COUNT          328 a          289 c          278 g          261 t
ORIGIN      
        1 attacatgcc ccatgaagaa aagaccgcgc gcgttggcac atgggtcttc ccgcgtgaga
       61 tcatcggctc cacaccgtgg ctgaagacgc tttgggatca acgtgaaaag attcagcaca
      121 tccccaccgc cattgtatgg ggcgactcgg atattgcatt caaggaaggt gagctaaaag
      181 tgtggaccag tctgatgaaa aatcatacgc tcaatgtctt gcacagaatc ggtcactacc
      241 cgcctgaaga agcgcctgaa gaagtcatca aggcattgaa agcatgaatc aaaccataga
      301 aaaagcgatt gacgcttatg gcggcagaga gttttggtta aaagcaaaac gtatcgaagc
      361 ggaagtcagc acacatgggt tggcgttcac gataaaaagc agacccacct tcaagcgcgc
      421 aaaaatctca atggagattg cccgcccgtt ttccaagctg atgcctattg ggaaaatcac
      481 acaactggct ggtgtgctgg atgaacatca tgtccgcctc gaagattcaa ctggcaggat
      541 cgttgaagaa cgaaaagatg cccgcaagta tttccctggc ggtctgcaac ggttattcca
      601 ttgggacgat ctggacatgg cctatttcgc caactatgcc ttctggaatt atttcaccct
      661 gcctgttttg ctcatgcgcg aggatattga ctggaaagag atcggacctg gctggctcga
      721 agcaaccttc cccgaaagca tccccaccca cagcaaaatt cagcgcttcc gctttgacct
      781 gaaaacgggt ctgctcattc aacatgacta taccgtagac atcatgagca aactggcaac
      841 cgctgcaaac gtcgtcctcg agcacaaaca aggtgctggt ttcttatacc cctcaaggag
      901 actggtcaca ccgcgctcgc catctggaaa gcccctcccc gccccaactt tgatcgatat
      961 tacagtgcat gagtttcaag tcaaataaca agtgcagctt tgagcagtat aaaatattaa
     1021 ttctgcaggg aatttataga gtaacgcggg gtgtttcccc gcgttactgt gcttacaacg
     1081 gttttttgcc tggtcaggag gctagtaaaa cagaaaggct gtgattaaat acatcagtcc
     1141 gagagtaaca gggaaa
//
LOCUS       DAOQLC010000435         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_112747_length_1156_cov_20.824705, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000435 DAOQLC010000000
VERSION     DAOQLC010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112747_length_1156_cov_20.824705"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1156)
                     /locus_tag="PL000_23280"
     CDS             complement(<1..>1156)
                     /locus_tag="PL000_23280"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC91855.1"
                     /translation="EPAATEAAEAPAATEAPAATEAPAGLPEGDGKVEIFGGYGEAQA
                     AAFQQALTEFGEANGIEITFTSLASFDTDIKVKIEAGQEPDIAMWPQPGGLKALAADN
                     LVPLEEVFDISVPQSTLVPGWDTLAVVDGKIYGLPVSANMKTLVFYNPSAFEAAGYSV
                     PTNEAELKALEAQIIADGSGYPWCAGIESGGATGWPFTDWLEQYVLDLNGPEVYAQWI
                     AGDVDFASPEITAAAELVAARLLAEGQVNGGGVAMATTSFGDNAALFANGAAEGQCFM
                     LRQGSFIVSFMPDDIQAEVKAGDYSHLDVFALPAPEGAQAGVIGGGDLFGVFQGHVDQ
                     DVAKVAAFISSPDVLKYQVATGDISPHKTFDSSLYPNEMQKKIGEAMAAAA"
BASE COUNT          299 a          305 c          323 g          229 t
ORIGIN      
        1 gcagcagcag ccatggcttc accgatcttc ttctgcattt cgttcgggta gagagaagaa
       61 tcgaaggtct tgtgcgggga aatgtcacca gtggcaactt gatacttcag aacgtcaggg
      121 ctggagataa aggcggcaac cttggcaaca tcctgatcca catgaccttg gaacacaccg
      181 aagaggtcac caccaccgat cacaccagct tgagcacctt caggtgcggg cagcgcaaac
      241 acatcgaggt ggctatagtc accggctttg acttcagctt gaatgtcatc gggcatgaag
      301 gagacgatga aggaaccctg gcggagcatg aagcattgac cttcagcggc gccattggcg
      361 aacagagcag cattgtcacc aaagctggtg gttgccatgg cgacgccacc accgtttacc
      421 tgaccttcag cgagaaggcg ggcagcaacg agttcagcag cagcggtgat ttcgggggaa
      481 gcaaagtcaa catcgccagc gatccactgg gcatatactt caggaccatt caaatcaaga
      541 acgtactgtt ccagccagtc ggtgaagggc caacccgtag cgccaccgga ttcaataccc
      601 gcacaccaag ggtaaccaga accatcggca atgatctgag cttcgagagc cttcaattca
      661 gcttcgttgg taggaacaga atagccagcc gcttcgaaag cagatgggtt gtagaagacc
      721 aaggtcttca tgttagcaga aacaggcaaa ccatagatct tgccatccac aacagcgagg
      781 gtatcccaac cgggaaccag ggtgctctgc ggaacgctga tatcgaatac ttcttccaaa
      841 ggaacaaggt tatcagcagc aagcgccttc aaaccaccgg gctgaggcca catagcgata
      901 tcgggttcct gaccggcttc gattttcact ttgatgtcgg tgtcgaagga agcgagggaa
      961 gtgaaggtaa tttcaatgcc attggcttcg ccaaattcgg tgagagcttg ttggaaagca
     1021 gcagcctgag cttcgccata accaccaaat atctcaactt ttccatcgcc ctcaggaagt
     1081 ccggcggggg cttcagtggc agcgggggct tcggtggcgg caggagcctc ggcggcttca
     1141 gtggcagcgg gttctt
//
LOCUS       DAOQLC010000436         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_112946_length_1155_cov_12.350909, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000436 DAOQLC010000000
VERSION     DAOQLC010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112946_length_1155_cov_12.350909"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..412)
                     /locus_tag="PL000_23285"
     CDS             complement(143..412)
                     /locus_tag="PL000_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91856.1"
                     /translation="MTPEQRIDAALDSVLKASGSALRHYTMPKTLADMREAMRKAMFD
                     EYIHKQCHDCGQFLPRDRWVRTDDPYKAHGLCRDCGIMYDDPAFM"
     gene            complement(441..1097)
                     /locus_tag="PL000_23290"
     CDS             complement(441..1097)
                     /locus_tag="PL000_23290"
                     /inference="COORDINATES: protein motif:HMM:NF016921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HNC91857.1"
                     /translation="MSPYSIIYADPAWRYGDKRKTLKGYSGAETHYKTMSISEIKELP
                     VREISSENSALFIWVVMPLLQEGLDVIKAWGFKYKTCAFSWMKTNKSSDKLFGRGVGS
                     YTMPNIELCLLGVRGKFFRNKKNVYQALLHPRMEHSKKPDEIRNRIVELCGDLPRIEL
                     FARQRVAGWDVFGNQVEGSIRLPTPLALDGGDSAALQALSTPEVLSTLQGEFTPAHRK
                     "
BASE COUNT          263 a          298 c          260 g          334 t
ORIGIN      
        1 cgcaggtgag acacttgcag tggtgcgcaa ggtgcgcgtt gtgctcgaat atgagctggg
       61 ttctcttgcg tcccatcagc aactcccgtc gccctcgatg ttgcggacgg ctttcagccg
      121 ccggttattt ctgcgttagg cgtcacatga acgctggatc gtcgtacatg atgccgcaat
      181 ctctgcatag tccgtgtgcc ttgtacggat catcggtcct cacccacctg tcacgcggca
      241 ggaattgccc acagtcgtgg cattgtttgt ggatgtattc gtcgaacatc gccttgcgca
      301 tcgcctcgcg catgtctgcc aaagtcttcg gcatcgtgta atgcctgagc gccgaaccgg
      361 atgctttcag caccgagtca agagccgcgt caattcgttg ttctggtgtc actttaaatc
      421 ctcctgcata acggtttgct ttacttgcgg tgggcgggtg taaactcgcc ctgcaaggtt
      481 gataaaactt cgggtgtaga taacgcctgt aaagcggcag aatccccacc gtcaagtgca
      541 agcggtgttg gaaggcgtat agaaccttcg acctgattgc cgaacacatc ccaacccgct
      601 actctttgcc gagcgaataa ctcaatgcgt ggaaggtcgc cgcacaactc aacgatacga
      661 tttctaattt catctggctt tttgctatgc tccattctgg gatgaagtaa agcctgatat
      721 acattctttt tattcctgaa aaacttacca cgaacaccaa gaaggcataa ttcaatgttt
      781 ggcattgtat aactgcctac accacgccca aaaagtttat cgcttgactt atttgttttc
      841 atccaactaa acgcacaagt cttatatttg aacccccaag ccttgataac atctaaacct
      901 tcttgtaaaa gtggcattac aacccaaata aacaaggctg aattttctga tgatatttcg
      961 cgcactggta attcttttat ttcagatatg ctcattgttt tgtagtgtgt ttctgcacca
     1021 gaataaccct ttagtgtttt tcgcttatcg ccataacgcc atgctgggtc tgcgtagatt
     1081 atgctataag gtgacattgc ttatgcctca cagttcgcgc accgtgattt cgtacttcgg
     1141 cttgccgttg cacgt
//
LOCUS       DAOQLC010000437         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_113380_length_1153_cov_26.724044, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000437 DAOQLC010000000
VERSION     DAOQLC010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113380_length_1153_cov_26.724044"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PL000_23295"
     CDS             <1..953
                     /locus_tag="PL000_23295"
                     /inference="COORDINATES: protein motif:HMM:NF025080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC91858.1"
                     /translation="LNAFYRGGPYGKFMYFPLFVFDENGWLLVAALRPGDHGEVKWSL
                     PILKRLVRRLRQAWPRVEIVVRADGAFTDNELYKWMDENRVRYVLGMKDNHSLFVKTK
                     QFRAKAQSKFKRKYEEPRYLGKSGKKAKNKRLKEISSTYDKDKRQQARQEFEHRRARV
                     YGETSYAARSWDRERRVIARCDYTDSGLEVRYVVTNLTGYQARQVYEDVYCRRARCEM
                     WIKSIKETRCDRLSCSQFKSNMFRLLMHALAYILIHQVQIRLEQNGSVTQFQRTFIHV
                     AVRVRETRKGVEFQIARSYHAARTFRLVSKRLGAKTPLAA"
BASE COUNT          354 a          226 c          331 g          242 t
ORIGIN      
        1 aattgaacgc attctacaga ggcggtccat atgggaagtt catgtatttt ccgttgtttg
       61 tgtttgatga gaatggctgg ttgctggttg cagcgctacg tccaggggat catggagaag
      121 tgaaatggtc gctgccgatc ctgaaaagac tggtgagacg gttaagacaa gcatggccgc
      181 gggtggaaat tgtagtgaga gcggatgggg cattcacgga caatgagcta tacaagtgga
      241 tggatgagaa tcgagttcgg tacgtactgg gcatgaagga caatcattcg ctttttgtca
      301 aaacgaaaca gtttcgagcc aaagcacaga gcaagttcaa aaggaaatac gaggagccca
      361 gatatcttgg caaaagtggg aaaaaggcaa agaacaaaag gctgaaagag atatcgtcaa
      421 cgtacgacaa ggacaagcgc cagcaggcaa gacaagaatt cgaacaccgg cgggcgcggg
      481 tgtatggaga aaccagttac gcagcccgtt catgggaccg ggagcgaaga gtaattgcca
      541 ggtgtgatta cacagacagc gggctggaag tgagatatgt ggtaacaaat ctcacaggat
      601 atcaggcaag acaggtgtat gaggacgtgt actgcaggcg ggccagatgc gagatgtgga
      661 tcaagtcaat caaagagaca cgatgcgatc ggcttagttg cagccagttt aagtcgaata
      721 tgttcagact gttgatgcat gcactggcat acatattaat ccaccaagtg caaatcagac
      781 tagagcaaaa tggcagtgtt acccagtttc agcgcacatt catacatgtt gcggtgagag
      841 tgcgcgagac gcgaaaaggg gttgaatttc aaatagcgag aagctatcac gccgcacgaa
      901 cgttcaggct ggtgtcaaag aggctagggg ccaaaacacc gttagctgct tgatctaagc
      961 aacaatggaa acttcacaac ctcagcagct gcattgggaa aaatcactag aatactgatt
     1021 gtagagctgt ttgagcctgg ttaaagtgaa aaatacgaca tggaaacgaa cccggctcac
     1081 tagtgaccac ctcttgggat gccatttctc gcggagatca cttctcgaca actatatgta
     1141 tgggtagacc agg
//
LOCUS       DAOQLC010000438         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_113382_length_1153_cov_15.159381, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000438 DAOQLC010000000
VERSION     DAOQLC010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113382_length_1153_cov_15.159381"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1119)
                     /locus_tag="PL000_23300"
     CDS             complement(610..1119)
                     /locus_tag="PL000_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91859.1"
                     /translation="MGALERLAEAQISSNLADDSLKLSDVDYLRASGWAAQINPDGLM
                     LYRLKYANDHREYAQTLRRVYSLAVGKAFRMRLTISHQDLHELAENTLRHWVAPICPS
                     CLGRGYEKRPDAPMLTDKECSHCKGAGHLPLERAVKSNLKLAEWLALKLDSSMGAFIA
                     SARNATETY"
BASE COUNT          248 a          312 c          312 g          281 t
ORIGIN      
        1 tccattgtgc agctccaaag caaaaagccc cggaaccgtt aggaaccgag gctttcgtgg
       61 tgatgcgagt gtgtgtaagg ctgtaagata aaaattctta ccttacgccg tcatcgggct
      121 attccgtgct aagtccggct ttcggaataa agaacccgcc aggttcgccc tacttggatt
      181 ggctcagttg gtcgcgtcca aatagctagg cgtggcgggt gttgttggtg ccggttacgc
      241 tatccggcga agcggtcagc agtgagctgg cttccgcatt agtggcctag tgcgccccct
      301 cagtaagccc aagggctagg ccggatgcct gatgctttcg cagcgcagga acgggttata
      361 ggtgacaggc atcgaaacct gcaactgcca gccaaacggc tagccgtgtc atccttacac
      421 tacacctata cggctggcga ctcgggacct actatccaag ttcggcgcga caccttggcg
      481 tatcgctttt gtgaatcgcc atgcgtatag acgcacagtg gccgggacaa tccggcctct
      541 tgtttgaatt cttgttgagt gcctacaaaa tactacatct agctccaaat tgcaagcact
      601 ctactaaatc tagtatgttt cagtcgcgtt tctggctgaa gcgatgaaag cgcccataga
      661 cgaatccagt ttaagcgcca accactcagc caacttgaga ttgcttttaa cggctcgttc
      721 cagcggcaag tgtcccgcgc ctttgcagtg actgcattcc ttgtcggtga gcattggcgc
      781 atctgggcgc ttttcgtacc ccctgccaag gcaggacggg cagataggcg ctacccaatg
      841 cctcagcgtg ttttcagcga gttcgtgcaa gtcctggtgg gagatggtca ggcgcatacg
      901 gaaagccttg cctacggcca aggaatagac acggcgcagc gtttgggcgt actcacgatg
      961 atcgtttgcg tacttcagcc gatacagcat cagaccatca gggttgatct gcgccgccca
     1021 tccagaagcg cggaggtaat caacgtcgga caacttcaac gagtcgtctg caaggttgct
     1081 gctgatctgt gcttctgcaa gtctctctag tgcgcccatg tcttatcctt attgttccga
     1141 gttttagtac agt
//
LOCUS       DAOQLC010000439         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_116014_length_1140_cov_24.238710, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000439 DAOQLC010000000
VERSION     DAOQLC010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116014_length_1140_cov_24.238710"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PL000_23305"
     CDS             complement(<1..338)
                     /locus_tag="PL000_23305"
                     /inference="COORDINATES: protein motif:HMM:NF012490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC91860.1"
                     /translation="MSHTSFATTTRGLNRDLPPMRLYEKAKKLGIWNPSDIDLSKDKL
                     DWARFTDEEKDLCLLLLSMFVAGEEAVTLDLLPLIQAVAHEGRIEEEMYLTTFLFEEA
                     KHTDFFRRFM"
     gene            661..867
                     /locus_tag="PL000_23310"
     CDS             661..867
                     /locus_tag="PL000_23310"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HNC91861.1"
                     /translation="MSRRITAALLAFFLGGLGVHKFYLGDMKMGVIYLIFCWTGIPAI
                     LGLIDLIQYLRVSDAEFDVKYPAK"
     gene            complement(946..>1140)
                     /locus_tag="PL000_23315"
     CDS             complement(946..>1140)
                     /locus_tag="PL000_23315"
                     /inference="COORDINATES: protein motif:HMM:NF017365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HNC91862.1"
                     /translation="ACLYGDGKLQVGQIWKQQSVVGSIFEGSIQIQDNRIIPTITGEA
                     WVMSEGTILVDERDPFGSGI"
BASE COUNT          287 a          255 c          259 g          339 t
ORIGIN      
        1 tccataaacc ttctgaagaa atcggtgtgc ttggcttctt caaagagaaa agtggtgagg
       61 tacatttcct cttcgatgcg gccttcatgt gcaacggcct ggatcaacgg cagcaggtcg
      121 agggtcacgg cttcttcgcc agccacgaac atggagagca gcaacagaca cagatccttt
      181 tcttcatcgg tgaacctggc ccaatccaat ttgtcttttg aaagatcaat atcggatgga
      241 ttccaaatgc cgagtttctt ggctttttcg tacaaccgca tgggcggcag gtcgcgattc
      301 aatccgcggg tggtggtggc aaaggaggtg tgggacatgg agggctccgt atcggtgctt
      361 gaagtgaaat atggtctcgt agattataca gccctatctt gtcggttcat catgggatgg
      421 attttgattg tacttttatc aacaatccgt tgctttttta cgaagccaat aagaccaatc
      481 ctgtctttta tggatataat cgcaaatcat acattccaat gaagaggaga gataccacag
      541 aaaattttcc atcacccaat attttcatcc ccgctggttt gcccgtaaga tgctcttgca
      601 atttccaaaa ttgcataaga tgccatcgtc actttaaact tgataataag gagatgtaaa
      661 atgtccagaa gaattaccgc agcgctgttg gctttttttc ttggtggtct tggtgttcac
      721 aagttctatc ttggcgatat gaaaatgggt gtgatttacc tgatattctg ttggacgggc
      781 atccccgcaa ttcttggatt gatagattta attcagtatc tccgtgtaag cgatgcggaa
      841 tttgacgtga agtaccctgc caaataaatc ttctgtttgg tttataaaga tagacctccg
      901 cgtatttcaa cgcggaggtc tttttgtaac aaaagatgac ctgttttata tgccgcttcc
      961 aaatggatcg cgttcatcca ccagaatcgt cccttcggac atgacccacg cttcgcccgt
     1021 aatcgttggg atgattcggt tatcctgaat ctgaatgctg ccctcgaaga tactgccaac
     1081 aacgctttgc tgtttccaga tttgaccgac ttgcagtttt ccatcgccgt acaaacaagc
//
LOCUS       DAOQLC010000440         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_116238_length_1139_cov_20.833026, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000440 DAOQLC010000000
VERSION     DAOQLC010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116238_length_1139_cov_20.833026"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..621
                     /locus_tag="PL000_23320"
     CDS             148..621
                     /locus_tag="PL000_23320"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNC91863.1"
                     /translation="MANSTKTELKTRALMAVRDYGVNLTHFRNAMNEWAGLNATDMEC
                     LRLLSQKGTATPTELAKHTGLTSGATTAMLDRLEKAGLIERRPNPNDRRGTLIAPEKS
                     SSAKMAAWFESARNAQDALMSTYSESELEIIADVFERFARLWDEEREKVQTASRS"
     gene            complement(600..>1139)
                     /locus_tag="PL000_23325"
     CDS             complement(600..>1139)
                     /locus_tag="PL000_23325"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNC91864.1"
                     /translation="GVIQGPGGPEEDTSGGFTLGGWISAHGDATVGAALRKQMNSSFD
                     LLVGRKTFEIWEPYWPQHADVWPNVNLATKYVASNTRTASDWKLSVFLSGDVANEVKK
                     LKQQDGPDLHVWGSGNLLQTLIKHDLVDVFWLMIYPVTLGSGKRLFADGTIPLKFKVT
                     ESMVGTSGVIVVNYERDAV"
BASE COUNT          301 a          309 c          249 g          280 t
ORIGIN      
        1 gattttgagg cgcttttttt agtatcggtc tttgtgctca taaaaatctc cttatctgac
       61 ttggtcattt tgacacttgc gtcaaaatca atttagttat ataataaaga tactttatta
      121 taaagataat gtcaagggta aatttctatg gcaaactcaa caaagactga attgaaaaca
      181 agggcattga tggcagtgcg agattacggg gtaaacctta cccattttcg caacgccatg
      241 aacgaatggg cagggctcaa cgccaccgac atggaatgtc tgaggctgtt atcgcaaaaa
      301 ggcactgcca cccctaccga acttgccaaa cacacgggcc tcacctcggg tgcaactacc
      361 gccatgctgg accgcctcga aaaggctggc ttgatcgaac gccgccccaa ccccaatgac
      421 cgtcgcggca cactcatcgc gcctgaaaaa tcaagcagtg ccaaaatggc tgcatggttt
      481 gagtccgcgc ggaatgcgca agatgcgttg atgtccactt attccgaaag cgagttggag
      541 atcatcgccg atgtattcga acgctttgcc aggctatggg atgaagaacg cgagaaggtt
      601 caaactgcat cacgctcgta attcacgacg atcactccgc tcgtgccaac catactttca
      661 gtcactttga attttagtgg gatcgtgcca tcggcgaaca ggcgttttcc acttcccaag
      721 gtaactgggt aaatcatcaa ccagaacaca tcaaccagat catgtttgat aagcgtttga
      781 agcaagttgc cactgcccca cacatgcaag tctggtccat cttgctgctt gagcttctta
      841 acttcgtttg cgacatctcc acttaaaaac acagacaact tccagtcact ggcagttctg
      901 gtattcgaag caacatactt ggtcgccaaa ttgacgtttg gccatacatc ggcatgctgc
      961 ggccagtacg gctcccaaat ctcaaaggtt ttgcgcccca ccaacaggtc aaacgacgaa
     1021 ttcatttgct ttctcagtgc cgctcccaca gttgcatcgc catgcgccga tatccagccg
     1081 ccaagcgtga atccgccgct ggtatcttct tcaggtccgc ctggaccttg aatcacacc
//
LOCUS       DAOQLC010000441         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_116443_length_1138_cov_22.297322, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000441 DAOQLC010000000
VERSION     DAOQLC010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116443_length_1138_cov_22.297322"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..303)
                     /locus_tag="PL000_23330"
     CDS             complement(7..303)
                     /locus_tag="PL000_23330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91865.1"
                     /translation="MSTHINDLREQLMNTLADLRNRSNPMEPDRARAIATVAGVLVDT
                     ARVEVDYIRATGQDTSNFIDSLKTPKAQAAITNAPTGKVDRSTPGVTRHTMGDD"
     gene            complement(300..>1138)
                     /locus_tag="PL000_23335"
     CDS             complement(300..>1138)
                     /locus_tag="PL000_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNC91866.1"
                     /translation="LFYTMPKASTWTPEMLAYVATHYPNSSTKAIAAHLGKSPLQICQ
                     KAYELNVKKSKAARGRSTDKWRVLDEIIALIYADMHNHDIEEFLGIKHADLISRACML
                     GLKKSKKIMREVAQRRAVTLSGEHRFQPGLVPWNKGMRGFDPVLGRGLYRNGLGAPHA
                     VPIGTTRLRGATPQNPKAREYLEKKVAEPSVWVRVHRLVWEQANGPVPDEHIIVFRPG
                     QYTTKEKEITVDRLECITRVELATRNHPMNHHPELAPIYQLKSAISRQINRIKQEQKE
                     AA"
BASE COUNT          174 a          329 c          323 g          312 t
ORIGIN      
        1 tcatgctcag tcatccccca tcgtgtgccg cgtcaccccc ggcgtgctcc tgtccacctt
       61 gcccgtgggt gcgttcgtga ttgccgcctg cgccttgggc gttttgagtg agtcgataaa
      121 gttgctggtg tcctggcctg ttgcccggat gtagtccacc tccacccttg ctgtgtccac
      181 cagcaccccg gccacggtgg cgatggcgcg cgcccggtcg ggctccatgg ggttgctgcg
      241 gttgcgcagg tctgccaggg tgttcatcag ttgttcccgc aggtcgttga tgtgcgtgct
      301 catgctgctt ctttctgttc ttgcttgatg cggttgatct gtctggaaat cgcgcttttg
      361 agctgatata tgggagctag ctcggggtgg tggttcatcg ggtgattgcg cgttgccaat
      421 tcaacccgcg tgatgcactc cagacgatcc acggtgattt ctttttcttt tgtcgtgtat
      481 tgccctggtc tgaaaacgat gatgtgttca tcgggtacag ggccgttcgc ctgttcccac
      541 accagccggt gcactcgtac ccatacgctc ggctcggcca ccttcttttc caggtactca
      601 cgagctttcg ggttttgcgg tgttgcaccg cgcaacctgg ttgtcccaat cgggacggcg
      661 tgcggtgctc caagtccatt gcgatacaag cccctgccca gcactggatc aaagccgcgc
      721 atccccttgt tccaaggcac aagcccaggc tggaaccgat gttctcccga taaggtcacg
      781 gctcggcgct gtgccacttc acgcatgatc tttttggact ttttcaagcc caacatgcaa
      841 gcgcgtgaaa tcaggtcagc gtgcttgata ccgagaaatt cctcaatatc gtggttgtgc
      901 atgtcggcat agatcagggc gatgatttcg tccagcactc gccatttgtc agtgctgcgg
      961 cctcttgctg cctttgactt cttcacattc agctcgtagg ctttttggca tatctgcagc
     1021 ggcgacttgc caagatgcgc cgcaatcgct ttggtgctgc tgttggggta gtgcgtggca
     1081 acgtaggcca gcatttccgg cgtccatgtg cttgctttgg gcatggtgta aaaaagcc
//
LOCUS       DAOQLC010000442         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_117474_length_1133_cov_19.925788, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000442 DAOQLC010000000
VERSION     DAOQLC010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117474_length_1133_cov_19.925788"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PL000_23340"
     CDS             <1..887
                     /locus_tag="PL000_23340"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC91867.1"
                     /translation="VTTVLAVEEMAFGDLAGALAVMTPSLFATPILLGGTEEQKQEYL
                     PKIIEGAWAPYTGALIEYSFNFDPNELKTTANAEGGSYVLNGEKAFVPFAKDAEALIV
                     YANLNGVTQGFIVKKEAAGLSISDEREKLMGLNALPMYRVKLENVTAERLGGASGHDF
                     GLILDAMRVASAATALGVAKSSLDYAKNYAKEREAFGVKIAQKQAIAFMLAEMAVELE
                     SMRLMTWEAAWKIDNNKEDSCVSAYLAFTGACDMAMMVTDRGVQIYGGHGYIRENPVE
                     LLMRNGRGFPMLLGLAIV"
BASE COUNT          280 a          294 c          302 g          257 t
ORIGIN      
        1 ctgtgaccac cgtcctcgct gtggaagaaa tggccttcgg cgatctcgct ggcgctctcg
       61 ccgtgatgac cccgtcgctg tttgccactc ccattttact gggcggcacc gaagaacaga
      121 agcaggaata tcttcccaag atcattgaag gtgcatgggc tccctacacc ggcgcactca
      181 tcgaatacag tttcaacttt gacccaaacg aactcaagac cactgccaac gccgaaggtg
      241 gttcttatgt tttgaacggc gagaaagctt tcgtgccgtt tgccaaagac gccgaagcgc
      301 tcatcgtgta cgcaaatctc aatggggtca ctcaaggctt catcgtcaag aaagaggcgg
      361 cgggtttatc catctctgat gaacgcgaaa aactgatggg actcaatgcc ctgcccatgt
      421 accgcgtcaa actggagaat gtgaccgccg aacgactcgg cggcgcctca ggccacgact
      481 ttggcttgat cctcgacgcg atgagagttg cctccgctgc gaccgctttg ggcgtagcca
      541 agtcctcgct ggattatgca aagaattacg ccaaggaacg tgaagccttc ggcgtgaaga
      601 tcgcgcagaa gcaggcgatt gctttcatgc tggcagagat ggcggtcgaa cttgaatcca
      661 tgcgtttgat gacgtgggaa gccgcttgga agatcgacaa caataaagaa gatagttgtg
      721 tttctgctta ccttgccttc accggcgctt gcgacatggc aatgatggtg accgaccgtg
      781 gcgtgcaaat ttacggtggt catggttata tccgcgagaa ccccgtggaa ttgctcatgc
      841 gtaacgggcg cggtttccca atgcttttag gacttgcaat tgtgtagaac cagttacagg
      901 ttacaagtcg caagttacag gttgtatgaa aacgttcaac ctgcaccctg caacctttga
      961 cctaaaggag agaatcatga ccatcgaatt tgaagccccg aagcccatcg tccaaatgca
     1021 ggcgatgttg aagactgtcg cagagaatat gatgcgcagc acctcgcgtg aaatggatga
     1081 gaacgaacat gccattccgt gggattacgt tgaatttatg cacactgcca tgc
//
LOCUS       DAOQLC010000443         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_118521_length_1128_cov_16.997204, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000443 DAOQLC010000000
VERSION     DAOQLC010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118521_length_1128_cov_16.997204"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PL000_23345"
     CDS             <1..662
                     /locus_tag="PL000_23345"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC91868.1"
                     /translation="KIWKPLGLHVKDHQGKLVDVDDVRLSPIWETAGELGFPVLIHVA
                     DPVAFFDPIDEANERWEEIGEHPDWAFTSPPFPPFMHIMNCFKNLVTRHSSTTFIGAH
                     VGCYGENLGWVGQMLDDCPNYYIDISARFGEIGRQPYTSRRFFIQYQDRILYGSDMSP
                     DLDAYRLSYRLLETDDEYFNYNTSEVPGQGRFYAYGLYLPDDVLKKVYRENAMKVLKF
                     Q"
     gene            681..>1128
                     /locus_tag="PL000_23350"
     CDS             681..>1128
                     /locus_tag="PL000_23350"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNC91869.1"
                     /translation="MKLQNYHTYNEIKSQTQAWAQALDVVNASRSPKAGDYDQVIFTG
                     CGSTYYLSLAAATLYTELTGRSAHAIPGGELLLNSQTVLTNQKTLLVAISRSGTTTET
                     VKATEKFKAEKRGDVAVISNYNEVLSRMADFNFVIDKGQEESVAQTR"
BASE COUNT          289 a          301 c          261 g          277 t
ORIGIN      
        1 tcaaaatttg gaagccgctt ggcttgcatg tcaaagacca tcaaggcaaa ttggtggatg
       61 tggatgatgt gcgtctctcc cccatttggg aaaccgcggg agaacttggc tttcctgtgc
      121 tcattcatgt cgctgacccc gttgcgttct ttgaccctat cgatgaagcc aatgaacgct
      181 gggaagagat cggcgaacat cccgattggg cgtttaccag tccgcccttc ccgccgttca
      241 tgcacatcat gaactgtttc aagaacctgg tcactcgcca ctcgtccaca acatttatcg
      301 gggcgcatgt aggttgttat ggcgaaaacc tcggctgggt cgggcaaatg ctcgacgatt
      361 gtccgaacta ctatattgat atctctgctc gcttcggcga gatcggacgc cagccttaca
      421 cctcccgccg tttctttatt caatatcaag accgtattct ttatggctct gatatgagcc
      481 ccgaccttga tgcctatcgt ctcagctacc gcctgctcga aacggacgac gaatatttca
      541 attacaacac cagcgaagtc cctgggcagg gacgcttcta tgcctatggc ttgtatctac
      601 ctgatgatgt cttgaaaaaa gtctatcgcg agaatgccat gaaggttttg aaattccagt
      661 aatcagtgaa atgcgaaacc atgaaactac aaaactacca cacctacaac gaaatcaaat
      721 cacagactca agcctgggca caggcattgg atgtagtgaa cgcttcacgc tcacccaaag
      781 ctggtgacta cgaccaggtc attttcactg gctgcggctc gacctactac ctctcgctgg
      841 ctgccgcaac tttatacacc gagttgacag gtcgttctgc acacgccatc cctggcggag
      901 agttgttgtt gaattcgcag acagttttga ccaaccagaa aacattactg gttgccatca
      961 gccgttctgg cacaacaacc gagaccgtaa aagcaaccga aaaattcaaa gcagaaaagc
     1021 gcggtgatgt tgccgtcatc agcaattaca acgaagtcct ttcgcgcatg gcggatttta
     1081 atttcgtgat cgataaggga caggaagaat ccgttgcaca gactcggt
//
LOCUS       DAOQLC010000444         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_119162_length_1125_cov_20.995327, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000444 DAOQLC010000000
VERSION     DAOQLC010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119162_length_1125_cov_20.995327"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PL000_23355"
     CDS             <1..227
                     /locus_tag="PL000_23355"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNC91870.1"
                     /translation="HGVSGSLLAFPIINQPQAGILGVGAMQKRVIVIDDAIAIRPMVY
                     LSFVFDHRILDGASADWFLAKVKETLENWS"
     gene            289..915
                     /locus_tag="PL000_23360"
     CDS             289..915
                     /locus_tag="PL000_23360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC91871.1"
                     /translation="MTNFDERAKDWDSDPKKVERARTVADAIRKAIPLSREMTALEYG
                     CGTGLLSFALQEDLGEITLADTSQGMLDVLTEKISASGVRNMKPLRLDLTTDPLPKER
                     FHLTYSLMTLHHVQDTEVILKKFADILLPNGILLVADLDKEDGSFHTDGTTDVHKGFA
                     RDALQKQVEANGFEDVKFSTAYIIKKKIGEEERTFPVFLMSARKRSDH"
     gene            905..>1125
                     /locus_tag="PL000_23365"
     CDS             905..>1125
                     /locus_tag="PL000_23365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91872.1"
                     /translation="MTTKILNGELEAYWEQGMEGLILFSFMPENGKGLNDLIALQSGQ
                     HLTIFNPDGSMLWSGTLNFVRRHDRRDQH"
BASE COUNT          308 a          328 c          274 g          215 t
ORIGIN      
        1 atcacggcgt gagcggatcg ctgctcgcct tccccatcat caaccaaccg caggcaggca
       61 tcctcggcgt tggcgcaatg cagaaacgcg tgatcgtcat cgatgacgcc atcgccatcc
      121 gcccgatggt gtacctgtct ttcgttttcg accatcgcat cctcgatggc gcatccgcag
      181 attggtttct ggcaaaggtc aaagagacat tggaaaactg gagttagcat gtcattgcga
      241 gggcgtactt tgcccgaagc aatctcctca caataacaac aaacaactat gacaaatttc
      301 gacgaacgcg ccaaagactg ggactccgac ccaaagaaag tggaacgcgc ccgcaccgta
      361 gcagacgcca tccgcaaagc cattccgctt tcacgcgaga tgaccgccct cgaatacgga
      421 tgcggcacag gcttgctgag ttttgcattg caggaagacc tcggcgaaat cacactggca
      481 gatacctcac agggaatgct cgacgtgctc accgaaaaga tctccgcctc aggtgtgcgg
      541 aatatgaaac cgcttcgcct cgacctgaca actgatccgc ttccgaagga aagattccac
      601 ctcacatatt ccctgatgac cctgcatcac gtacaggata ccgaagtcat cctgaaaaaa
      661 ttcgccgaca tccttctgcc aaacggcatc ctgctcgtcg ccgacctgga caaggaagac
      721 ggctccttcc acaccgacgg cacaaccgac gtccacaagg gttttgcgcg ggatgcgtta
      781 caaaaacaag tggaagcaaa cggctttgaa gacgtaaaat tttccactgc atacatcatc
      841 aaaaagaaga tcggcgaaga agaaaggacc ttccccgttt ttctgatgtc ggcgaggaag
      901 cgaagtgacc actaaaatac tgaacggcga attggaagcc tactgggagc agggcatgga
      961 aggattgatc ctgttcagtt tcatgcccga aaacggaaaa ggactcaacg acctgatcgc
     1021 actgcaaagc ggtcaacacc tgacgatctt caaccctgac ggcagcatgc tctggtcggg
     1081 cacactcaac tttgtccgca gacatgatcg gcgcgaccag cacaa
//
LOCUS       DAOQLC010000445         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_119626_length_1123_cov_20.351124, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000445 DAOQLC010000000
VERSION     DAOQLC010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119626_length_1123_cov_20.351124"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1123
                     /locus_tag="PL000_23370"
     CDS             98..1123
                     /locus_tag="PL000_23370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91873.1"
                     /translation="MTKPTGIIIIDGPDGSGKSTLAQRLVEQYDAEYLHLTYRWPNNM
                     FDYHTAAIHWAGKRSKDKLVVIDRWWMSELCYANAYRNGSKWPLMYRMLDRIALKYGC
                     IYVYSLPENIRDHLNAYEGLKLTREEMYKDISPVVIEYHKLWDKVKTWPHVRRYDYQK
                     HALQGCGENTVDAYAAALVADLQAWRAEQVDIALDPANYNLTGHISPAKYLLVGDKTN
                     PRKRRGNWWPFHDYAASSLHLAQTLERLGVPEYECLYTNYNNSKAETDYLMRNFDLRM
                     IVFGSAVFDPLVRDFGGGSKAWKILANPQNVVHPAYDLRFRHGKTLACDLGSAVLRSE
                     AYGRFPT"
BASE COUNT          286 a          283 c          305 g          249 t
ORIGIN      
        1 ctagcgcaga agaatccaag cggaagtctg gcttcgacgt agtgaaaccc gaaggctggc
       61 aaccgcccaa actccacgac ctggtgggag gcaactaatg accaagccga cagggattat
      121 cattatcgat ggccctgacg gcagtggaaa gtcaacactg gcacagcggt tggtagagca
      181 gtacgatgct gagtatctgc atctaaccta ccgctggccg aataatatgt tcgactacca
      241 cacagcagct attcattggg cggggaaacg ttccaaggat aagctggtcg tcatcgatcg
      301 ttggtggatg agcgagctgt gctacgcaaa tgcataccgc aatggctcta agtggccgtt
      361 gatgtatcgc atgttggatc ggatagcact caagtatggt tgtatatacg tctattcact
      421 tcccgagaat atccgagacc acctgaatgc ctatgagggc ctaaaattga ccagggagga
      481 gatgtataaa gacatttctc ctgtcgtgat tgaatatcac aagctctggg acaaggtaaa
      541 gacgtggcca cacgtacgtc gatacgatta ccaaaagcac gcgcttcagg gctgtgggga
      601 gaataccgta gacgcgtacg ctgcagccct tgtagccgat ctccaggctt ggcgcgccga
      661 gcaggttgat attgcgctgg atccagcgaa ctacaatctc acaggccata tctcgccagc
      721 gaagtatttg ttggttgggg acaaaacgaa tccacgtaag aggcgcggta actggtggcc
      781 cttccatgac tacgcggcgt caagtctcca cctggcccag acactggagc ggttgggcgt
      841 gcctgagtac gagtgcctgt ataccaacta caacaactca aaggctgaga ccgactacct
      901 gatgcgtaac ttcgacttgc gtatgattgt gttcgggtca gctgtatttg accctctagt
      961 tcgtgacttc ggcggaggct ctaaggcatg gaagattctc gcaaacccac aaaacgtcgt
     1021 tcaccctgcg tatgaccttc gtttcaggca tggcaaaacc ctggcctgtg accttggcag
     1081 cgcagttcta agatctgaag catacgggag gtttccaaca tga
//
LOCUS       DAOQLC010000446         1120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_120267_length_1120_cov_19.138028, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000446 DAOQLC010000000
VERSION     DAOQLC010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120267_length_1120_cov_19.138028"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..889)
                     /locus_tag="PL000_23375"
     CDS             complement(245..889)
                     /locus_tag="PL000_23375"
                     /inference="COORDINATES: protein motif:HMM:NF039197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HNC91874.1"
                     /translation="MTSENQEYLERFTDQISKFMPTAVLLEFDPVDEKAVQEKYDQYL
                     TGSFTLPSNEIYQLGFRIAKKSSLKTVYSFDERTIGWNAGPMFEYINTHDAETKSRFD
                     ALIEQIGETTKRAHETLSLAELLMDANAEETDRHNKYIYLITNHVGAGDNFEGADAAA
                     SWWHRNFRMYANIQKHAAPGERVLAIGGQGHTAILKDLLKIDEDREAVDFAQYL"
BASE COUNT          292 a          290 c          212 g          326 t
ORIGIN      
        1 ccactcgttt gccagttctt cagcggtaag cacaaaaagc gaatcgccct ccatttccgc
       61 aacatcatga ttgagttttg ctaccgcatt gacatctgtc caacgggcac ggcgtagcgt
      121 taggcttggg tcaagataaa tcttttcagt cataacaatc tccgctcacc ttcagtttac
      181 ctataaagtt cgtataaatt ttgcaagagc tgaccaacag accagcacac aaacggggtg
      241 ccctttataa atattgggca aaatcaaccg cctccctatc ttcatcaatt ttcaacaggt
      301 cttttaatat cgcagtatgc ccctgcccgc caatggccaa tactctttct ccaggggcgg
      361 catgcttttg aatattggca tacattctga agtttctatg ccaccagctc gccgccgcat
      421 cagcgccttc aaagttatct cctgcgccga catggttcgt gatcaagtat atatacttgt
      481 tgtgcctgtc tgtttcctcc gcattggcat ccatcaacaa ctcagccaga gacaatgttt
      541 catgtgcccg ctttgtcgtc tcgccgattt gttcgatcag ggcatcaaac cgagactttg
      601 tttccgcgtc atgtgtattg atgtattcaa acatgggccc ggcattccaa ccaatggtcc
      661 tttcatcaaa actgtacacg gttttcaggc tggatttttt tgcaatacgg aaaccgagtt
      721 gataaatctc attggagggc agggtaaaac tgcctgtgag atactggtca tatttctctt
      781 gaactgcctt ttcatctaca gggtcaaact caagcagcac ggcagtgggc atgaatttac
      841 tgatctgatc tgtaaaacgc tcaagatatt cctgattttc cgatgtcata acattgaggt
      901 gttccgtttt aactagatcc tgcatgggat ccataaaatg gaacaccccc aacaacataa
      961 cctttgcctt ttgttcagaa acaacaccgc tcatattatt cgcatccttg ataaaaaggg
     1021 ccatattttt tgaaacattt gatccaatgg gcagccaaag acaaaacgac agttacacga
     1081 ccttcttcct caagatcctg ttccaatcca tcatcgtctt
//
LOCUS       DAOQLC010000447         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_120499_length_1119_cov_18.823308, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000447 DAOQLC010000000
VERSION     DAOQLC010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120499_length_1119_cov_18.823308"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1119
                     /locus_tag="PL000_23380"
     CDS             <1..>1119
                     /locus_tag="PL000_23380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNC91875.1"
                     /translation="RLLKITEDRAAELAIINSVSEGLVRELDFQAIIDLVGEKIRQDF
                     KVEDMYIAMYDEASNLLSTPYYIEHGDRFPIEPMTLRPGYAGWTITNRATLVINENIV
                     QRKREMGMDTNLLIGDTQQAEDLTQSVVCAPIWSTGKVIGVITLYSNEPNAFPESSVS
                     LLTTLSANLGVALQNARLFDETQRLLKITEDRAAELAIINNVQQGLASKLNMQSIFDL
                     VGDKIRDMFSSQTMVIGAFDHEKQTSTVPYVFDEGQRVVDDEHIPFSQLVKHLITTRE
                     PVVINENAIEEAKHYGLTIIEGTQTPKSLIFVPYGTGSQVNGYFSLQNLERENTFTES
                     DVRLLQTLASSMGIALENARLFDETQRLLKVTEDRAAE"
BASE COUNT          316 a          321 c          255 g          227 t
ORIGIN      
        1 agcgcctgct caagatcacc gaagaccgcg ccgccgagct tgccatcatc aacagtgtca
       61 gtgaaggctt ggtgcgcgaa ctggacttcc aagccatcat tgacctggtc ggtgaaaaga
      121 tacggcaaga cttcaaagtt gaagatatgt acattgccat gtacgatgaa gccagtaatc
      181 tcctgtccac cccttattac atcgaacatg gtgaccgctt ccccatcgaa ccgatgacac
      241 ttagacctgg gtacgcaggt tggacaatta caaaccgcgc tacactggtc attaacgaaa
      301 atattgtcca gcgaaaaaga gaaatgggga tggatacgaa cctattgatc ggcgataccc
      361 aacaagcgga agatctaacc caaagtgttg tgtgtgcgcc catttggtca acgggcaagg
      421 tcatcggcgt catcacactc tactcgaatg aaccaaacgc cttcccagag tccagtgtca
      481 gtctgctcac gaccttgtca gccaacttgg gcgtggcgct gcaaaacgcc cgcctcttcg
      541 acgaaaccca gcgcctgctc aagatcaccg aagaccgcgc cgccgaactt gccatcatca
      601 acaatgtaca gcaggggctg gcgtccaaac tgaatatgca atccatcttc gaccttgtcg
      661 gcgacaagat ccgcgatatg ttcagttcac aaaccatggt cattggcgcg ttcgaccatg
      721 aaaaacaaac aagcaccgta ccttatgtgt tcgatgaagg tcaacgagtg gttgacgatg
      781 aacacattcc attcagccaa ctggtcaaac atctcatcac cacccgcgaa cccgtagtca
      841 tcaatgaaaa tgctatcgaa gaagccaaac actatggatt aaccatcatt gaaggcacgc
      901 aaacgcccaa gtccctgatc ttcgtcccat acggaacagg ctctcaggtc aacgggtatt
      961 tcagccttca aaatttagaa cgcgaaaaca ccttcacgga atcggacgtt cgtttgctgc
     1021 aaacgctggc gagcagtatg ggcattgcgc tcgaaaacgc ccgcctcttc gatgagaccc
     1081 aacgcctgct caaggtcacc gaagaccgcg ccgcagaat
//
LOCUS       DAOQLC010000448         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_120743_length_1118_cov_15.965193, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000448 DAOQLC010000000
VERSION     DAOQLC010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120743_length_1118_cov_15.965193"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..>1118)
                     /locus_tag="PL000_23385"
     CDS             complement(67..>1118)
                     /locus_tag="PL000_23385"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNC91876.1"
                     /translation="AASCTTLPANANVDANFSASSYVDGTALTGILAIPHEDFTAAGD
                     VACNTFEYWALASDSFGLANPTDPYSGFGSWNAQTTGTFAPAPIGAEDELLEWQITLP
                     ATATGTWVISIDPEDAAGNTNVLGPYDFRNTAIQANEIEDCADFTDVAGHENEVYVRY
                     LADLGLISGFADGSFGPDNTLTRAEAATLIEISNGYDATTLPTSAPAGCDFTDVAASD
                     WFAGWVWQACADGFMNGVGGGMFDPNNLLTRGQIVTIFNNIYELGVGNLQPGSHMVVG
                     LGLLAFFDTSPRFREAAWSDVSLGAYYAEPVVEAYSWGIVDGTSETTFSPDQAMTRGE
                     FAKTLYRILAYYSLF"
BASE COUNT          294 a          289 c          323 g          212 t
ORIGIN      
        1 aacaaaaaag gagcggacct gtacaaggtc cgctccagat tttcaatcta cactagcacg
       61 ggaaacttag aacaaagagt aataagccaa aattctgtag agagtcttgg cgaattcacc
      121 acgggtcatg gcctggtcag gcgagaaggt ggtttcgctg gtgccatcta caatgcccca
      181 actgtaggcc tccaccacag gttcggcata gtacgcgcca agggatacat cactccaggc
      241 cgcttcgcgg aaacgaggag aggtatcaaa aaaggctagc aggccaagac cgacaaccat
      301 atgagaacct ggctgaagat tacctacacc aagttcataa atattgttga aaatagtgac
      361 gatctgtcca cgtgtgagca ggttgttcgg gtcgaacatg ccgccgccca caccgttcat
      421 gaagccatct gcacaagcct gccagaccca gccagcgaac caatcggacg ccgccacgtc
      481 ggtgaagtca caaccagcag gggcagaagt cgggagggtg gtcgcatcat aaccgttgga
      541 gatctcgatc agggtggccg cttcggcgcg ggtcagggta ttgtcggggc cgaaagaacc
      601 atcggcgaag ccgctgatca ggccaaggtc agccagataa cgtacataca cttcgttctc
      661 atgaccagcc acatccgtaa agtcggcaca gtcttcaatt tcgttagcct gaatagcggt
      721 attccgaaaa tcataagggc caagaacgtt tgtattgcca gcggcatctt ctgggtcaat
      781 cgagatgacc caggtgccag tagcggtggc aggcagggtg atctgccatt cgagcagttc
      841 atcttccgcg ccgatcgggg ccggggcaaa agtgccagtc gtctgggcat tccacgaacc
      901 gaatccagag tatgggtcgg tggggttagc gaggccgaag ctatcggaag ccagtgccca
      961 gtattcaaag gtattgcagg ctacatcacc agcagctgtg aagtcctcat ggggtatggc
     1021 aaggatgccc gtcaacgcag tgccatcgac ataggaggag gcggagaagt tcgcatccac
     1081 attggcattg gcaggcaggg tggtgcagga agccgcat
//
LOCUS       DAOQLC010000449         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_124130_length_1103_cov_20.941794, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000449 DAOQLC010000000
VERSION     DAOQLC010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124130_length_1103_cov_20.941794"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1096
                     /locus_tag="PL000_23390"
     CDS             101..1096
                     /locus_tag="PL000_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNC91877.1"
                     /translation="MKHYKQLTSEQRYQISGLKKAGLNQSQIADAVGVNKSTISREFR
                     RNKGRRGWYPKQAEELRNERRKQCANAQRLSMSDWTEVERLVRLDMSPEQAARRLALE
                     SVLQISHETIYLHIYADKRQGGDLWRHLRCQKPRRKRYASGQERRGTIKNRIGIDERP
                     GIVDQKNRIGDWEGDTVIGKNHQGALVTLVERKSRYILAAQVPGKHASGVTAAVTRLL
                     RPHKRKCHTMTFDNGKEFAEHETIAAKLNVDIYFAHPYHSWERGLNENSNGLLRQYFP
                     KGMELIKVTQEQVQWAVDRLNHRPRKVLGFRTPFEVFFGKTVRYTKPPLGVALRS"
BASE COUNT          295 a          259 c          337 g          212 t
ORIGIN      
        1 tggtggattc aaaattgaag tgcaactttt gtaaggaagt ttaggaagca agctgcggta
       61 gtatcggagc ttcttaaacg accaagtaga aggagcacag atgaagcact acaagcagct
      121 caccagcgag caacgatacc agatttccgg cttgaagaag gcgggtttga accaatcgca
      181 aatagcggat gcagtgggcg tgaacaagtc gacgatttcg cgggaattca ggcggaacaa
      241 gggtcggcgt ggctggtatc ccaagcaggc tgaagaatta cggaacgagc gtcgaaaaca
      301 atgcgccaac gcccagcgtc tatcaatgtc ggactggacg gaagtcgagc gattggttcg
      361 gctggacatg agtccggagc aagcggcccg tcgacttgct ttggaaagtg tgttgcagat
      421 cagtcacgag acgatctact tgcacatata tgcagacaag cgtcaaggtg gcgacctgtg
      481 gcggcatttg cgttgccaga agccccgcag gaagcgttat gcgagcggtc aagagcgccg
      541 gggcacgatc aagaaccgga tcggcattga tgagcgtccg gggatcgtgg atcagaagaa
      601 ccgcataggc gactgggagg gcgacaccgt aatcggcaag aaccatcaag gcgcattggt
      661 tacgctggtc gagaggaagt cgcgctacat tctggcagcc caagtgcccg gcaaacatgc
      721 atcgggcgta acggcggcgg taacgcgatt gctgcgcccc cataaacgca agtgccacac
      781 catgacattc gacaacggga aagaatttgc ggagcatgaa actattgctg cgaaactcaa
      841 tgtggatatc tacttcgctc atccttatca ttcatgggag cgcggcttga acgagaacag
      901 caatgggctg ctgcggcagt atttccccaa ggggatggaa ttgattaagg tgacccagga
      961 acaagtacaa tgggcggtgg atagactcaa ccatcgaccg cgcaaggtgc ttggattcag
     1021 aaccccgttt gaggtgttct tcggaaagac ggtgcgctat accaagccac cgttaggtgt
     1081 tgcacttcga agttgaatcc gcg
//
LOCUS       DAOQLC010000450         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_124364_length_1102_cov_10.753582, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000450 DAOQLC010000000
VERSION     DAOQLC010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124364_length_1102_cov_10.753582"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1005
                     /locus_tag="PL000_23395"
     CDS             79..1005
                     /locus_tag="PL000_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNC91878.1"
                     /translation="MNPEIISWLLEGDPSIRWQVQRDLLHASQSKYENERKRIAREGW
                     GARLLALQDPSGTWAGGLYGPKWTSTTYTMLTLRLLGLQQKHPQAKRACKIFLDEGYY
                     TDGGINFFSYSWTYSETCVTSMILALLTYFRHPDERIHNIASYVIGEQMPDGGWNCES
                     RKGATHSSFHTTISALEGLYEYECLFPEKKKIVNKVRARGHDFLLAHRLYKSHRTGKV
                     FDAKMTAMPFPPRWKYDFIRALDYFRACDAPRDERMSDAIELLKSKQRKDGTWLMNSG
                     MSGRKYFDLEKAGQPSKWNTLRAMRVLNWWNK"
BASE COUNT          302 a          304 c          260 g          236 t
ORIGIN      
        1 cactgaaagg cattctcaac gaaaccgccg cttaccgaaa atattttgca agcgctgagc
       61 tgaaaacact tttgcaatat gaaccctgag atcatttcct ggctgctcga aggtgaccca
      121 tccatccgct ggcaggtgca gcgcgatctg ttgcatgcat cgcaaagcaa gtatgaaaat
      181 gaaagaaaaa gaatcgccag ggaaggctgg ggcgcacgat tgcttgccct gcaagacccc
      241 agcggaacct gggctggcgg actctacggc cccaagtgga cttccaccac gtacacgatg
      301 ctgaccctgc gcctgctcgg actgcagcag aagcatccgc aggccaaacg ggcttgcaag
      361 atctttctgg atgaaggcta ttacacagac gggggcatca actttttttc atactcatgg
      421 acctacagtg aaacctgcgt caccagcatg atccttgccc tgctcactta tttccgccac
      481 cccgatgaac gaattcacaa catcgcttca tacgtgatcg gagaacaaat gcctgatggc
      541 ggctggaatt gcgaatcgcg caaaggcgca acccactcct catttcacac cacgatctcg
      601 gccctggaag gattgtacga atacgagtgt cttttccccg aaaagaaaaa aatcgtcaac
      661 aaggtccgcg cccgtggaca tgatttcctg ctcgcgcatc ggctgtataa atctcaccgc
      721 acaggcaagg tcttcgacgc gaaaatgacc gccatgccct ttccgccccg ctggaaatat
      781 gacttcatcc gcgcattgga ttatttccgc gcctgtgatg ccccgcgcga tgaacgcatg
      841 tctgatgcca ttgaattatt gaaaagtaaa caaaggaaag acggcacctg gctgatgaac
      901 agcggcatgt ctggcagaaa atattttgac cttgaaaaag ctggtcaacc cagcaagtgg
      961 aatacgctca gggcaatgcg ggtgttgaat tggtggaata aataaagcct tacgaatcgt
     1021 aatcaataaa atcatccacc ccaaatagat cttcgtaacg ggtcaacagc accgcggtct
     1081 tgctcaaaaa ttcctgttcc gc
//
LOCUS       DAOQLC010000451         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_124572_length_1101_cov_19.591778, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000451 DAOQLC010000000
VERSION     DAOQLC010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124572_length_1101_cov_19.591778"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>1101)
                     /locus_tag="PL000_23400"
     CDS             complement(32..>1101)
                     /locus_tag="PL000_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012628135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNC91879.1"
                     /translation="GYLPERTIQTGLGDISVKVPKIRDRSKRGIKFNSELVPPYLKRT
                     KAVEEFLPWLYLRGISTGDFSEALQHLLGKDAPGLSAATISRLKQGWESDYQAWQKRD
                     LSKKRYAYIWADGVYSHVRQDDRLCLLVIIGSDERGQKELLALTDGYRESAASWEDVL
                     NDLMGRGLQTAPKLAVGDGALGFWKALSKLWPTTNQQRCWVHKSANVLEKLPKSVQPK
                     VKEALHNIWQAETREAALKSFDQCVKRFELKYPKAMECLNKDKDSMLAFYDYPAEHWP
                     HIRTTNPIESVFATVRLRTDRTKNCGSRDTTLAMAFKLMESAQKRWRRLRGYKMLADV
                     IVGVKFINGIKQKPDQKQKAA"
BASE COUNT          245 a          297 c          242 g          317 t
ORIGIN      
        1 tgagctatag tcaaatctgg tgtatagaga atcaagcggc cttctgtttt tgatccggtt
       61 tttgtttgat gccattaatg aattttacac ccacaataac gtcagccaac attttgtaac
      121 cacgcaggcg gcgccaacgt ttctgtgcac tttccatgag tttgaatgcc atggctaacg
      181 tagtatctcg gctgccacaa ttcttcgttc tatcagttcg aagtcgcacg gtagcaaaaa
      241 ccgattcaat cggattcgtc gttcgtatgt gtggccagtg ttctgctggg tagtcataaa
      301 atgcaagcat cgaatccttg tctttattca ggcattccat agcttttgga tatttgagtt
      361 caaagcgttt tacgcactga tcaaaggatt ttagcgctgc ctccctcgtt tccgcttgcc
      421 atatgttatg caacgcttct ttaactttgg gttgcaccga tttcggcagc ttttccagca
      481 cgtttgcact tttgtgcacc caacatcgtt gctgatttgt cgtgggccat agtttgctga
      541 gtgctttcca gaatcccaga gcgccatcac cgacggccaa cttgggagct gtctgcagac
      601 ctcgccccat caggtcgttt agcacgtctt cccaacttgc ggcggattcc ctgtagccat
      661 ctgtgagcgc cagcaattcc ttctgaccac gctcgtccga gccaatgatg accagcaaac
      721 acaatcgatc atcctgacgt acgtggctat aaacgccgtc tgcccaaata taggcataac
      781 gctttttgct caagtcccgc ttttgccaag cttggtaatc cgattcccag ccttgtttta
      841 gccgactaat ggtcgcagca gacaaaccgg gggcatcctt ccctaacaaa tgctgaagcg
      901 cttctgaaaa gtccccggtc gaaatcccgc gcagatataa ccaaggcaaa aactcctcga
      961 ccgccttggt gcgtttcaaa tacggcggca ccagttcact gttgaatttg atgcctcttt
     1021 tgcttcggtc ccgtatcttg ggtactttga cggaaatatc ccccagccct gtctgaatag
     1081 tgcgttccgg taagtaaccg t
//
LOCUS       DAOQLC010000452         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_125790_length_1096_cov_15.970221, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000452 DAOQLC010000000
VERSION     DAOQLC010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125790_length_1096_cov_15.970221"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PL000_23405"
     CDS             complement(<1..424)
                     /locus_tag="PL000_23405"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC91880.1"
                     /translation="MDLDLKNKIAIITGASRGIGKSIAQTLSAEGVKLTLVARSPLDE
                     FSASLPTESLPLSIDLRQPDAASRVVEATLQKFGGIDILVNNAGATKRGDFLQLTDDE
                     WTDGFALKFYGAMRLSRAAWTSLMQSKGSIVNIIGIGGR"
     gene            complement(455..931)
                     /locus_tag="PL000_23410"
     CDS             complement(455..931)
                     /locus_tag="PL000_23410"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNC91881.1"
                     /translation="MSIKIPAKVEKTINAADKIYRGCLWIAVNFFVMLFLCWALYSTV
                     VGLRVETNGASVEGYVVDQKTAPDGNVSAVVEFSVNGQTHRFEDDAASIPPRYDLGQK
                     VTVLYDRSDPSIARIDDRAPTWLFPACGLMTTILALIGINIWGVRAWKRGEEIIDL"
BASE COUNT          266 a          269 c          304 g          257 t
ORIGIN      
        1 tccgcccgcc aatgccgatg atgttgacaa tgctgccttt gctttgcatg agactcgtcc
       61 acgcggcgcg ggagaggcgc atggcgccgt agaacttgag ggcgaatccg tctgtccatt
      121 cgtcatcggt aagttgcagg aagtcaccgc gtttggtggc gcctgcatta ttgacgagga
      181 tatcgatccc gccgaatttt tggagcgtgg cttcgacgac gcgggaagcg gcatcgggtt
      241 ggcgaaggtc aatggataga ggcaggcttt cggttgggag agaggcggaa aattcatcca
      301 gcggggaacg tgccacgaga gtcagtttca ctccctcggc agataatgtc tgggcgatgg
      361 attttccgat cccgcggctg gcgccagtga tgatggcaat tttgtttttg agatccaagt
      421 ccatttcaaa ctccatgaat aaaaagcgga attgctaaag gtcaattatc tcttcgccgc
      481 gtttccaggc acgaaccccc caaatgttga tgccgatcaa ggcgaggatt gtggtcatta
      541 atccgcaggc ggggaacagc caggttgggg cgcggtcgtc aatacgagca atgctggggt
      601 cggagcgatc ataaagcacg gtgacctttt gtcccaaatc gtatcgcggc ggaatggatg
      661 cggcgtcatc ttcgaagcga tgggtctgcc cattgacgct gaactccacc acggcagaga
      721 catttccgtc tggggcggtt ttttgatcaa caacgtaacc ttccacagag gcgccattgg
      781 tttccacacg caggccgacc acagtggagt aaagtgccca acagagaaag agcatgacaa
      841 aaaaattgac cgcgatccag agacaccctc tgtaaatttt atcggcggcg ttgatggtct
      901 tctccacctt ggcagggatt ttgatactca aatacagcct cttctattgg aactcttgca
      961 taagttcatt tttcaccaca acacttgcgc cgcacgctag tgcaggtgag gcacaacgtc
     1021 tcgaagactc aaagaaaaaa gcttggtgac tttgtggtct acaacacttt acttatgcaa
     1081 gaggtcaatt ataaaa
//
LOCUS       DAOQLC010000453         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_126459_length_1093_cov_36.652216, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000453 DAOQLC010000000
VERSION     DAOQLC010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126459_length_1093_cov_36.652216"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="PL000_23415"
     CDS             complement(<1..950)
                     /locus_tag="PL000_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91882.1"
                     /translation="MPVLEHDIPNTYCTRNHEVQTGSFFVDHEFGKLVTVSFYRGLAY
                     GPKAAYAPNTTAYSSAMGQQAIGWGMGSAGESNYQFDDSCPYYKNPGGDQNTFPPGAV
                     DELNAALLEMGNAWISQTMITDSSHEGTNRTDFYYGSVNSSVTDLVASLSWETRDYLL
                     LDKTNSVLIYIKGEFSASKSAPDPAPGAATLTVTLCIETPWGSASQALYSLSYTYGTM
                     LPENDEIAPGKYAIPSPQIRAIFTPKYREQGSFFGAAYITKAEVANGATPACLINFAL
                     SLETYSFIGTDETDGVVHFIPCNLLEMLYAFVFSRRYGCDP"
BASE COUNT          240 a          288 c          303 g          262 t
ORIGIN      
        1 gggtcgcacc cataccgccg gctgaaaacg aaggcgtaga gcatctccag cagattgcac
       61 gggatgaagt ggacaacgcc gtctgtttca tccgttccga tgaacgagta cgtttccagc
      121 gacagggcga aattgataag gcacgccggt gtggcgccgt tggcgacctc tgccttggtg
      181 atgtaggcgg ctccgaaaaa cgaaccctgc tctcgatact tgggggtgaa gatggcccgg
      241 atttgcggag aggggatcgc gtactttcca ggagcgatct cgtcgttctc tggcagcatg
      301 gtcccgtagg tgtagctgag cgaatacagc gcctgagacg cgcttcccca cggggtttcg
      361 atgcacagcg ttaccgtcag ggttgcggct cccggtgccg gatcaggagc cgacttcgat
      421 gccgagaact cacctttgat gtagatcagg acgctgttcg tcttgtccag cagcaggtaa
      481 tccctggttt cccacgacag cgaggcaacg agatcagtca cgctgctgtt caccgaaccg
      541 taatagaagt ctgtccggtt tgtgccctcg tggctggaat ccgtgatcat ggtttgagaa
      601 atccacgcgt tccccatctc caataatgcc gcgttcagtt cgtcaactgc acctggagga
      661 aacgtattct gatccccgcc gggattttta taataaggac agctgtcatc aaactgataa
      721 ttcgattctc ccgcagagcc catgccccac ccgatggctt gctgccccat agcggaacta
      781 taggctgtgg tgtttggcgc ataagctgct ttggggccgt aggctaaacc cctatagaac
      841 gacacggtta cgagcttccc aaactcgtgg tcgacaaaaa acgaccctgt ctgcacttcg
      901 tggtttctgg tgcaataggt gttcggtata tcgtgctcta aaactggcat gtataaggat
      961 cccctatacg gcggaacggt gtccttcgta tatccgcttt gccatgacag cacgttacta
     1021 gagtttatta gaaaatcgtt gatgatattg gcaagacctc cggttgccaa agtaatggtg
     1081 gacgtattgc att
//
LOCUS       DAOQLC010000454         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_126929_length_1091_cov_18.104247, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000454 DAOQLC010000000
VERSION     DAOQLC010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126929_length_1091_cov_18.104247"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1091
                     /locus_tag="PL000_23420"
     CDS             <1..>1091
                     /locus_tag="PL000_23420"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HNC91883.1"
                     /translation="ASLFRVLLGWYEATRDERTLTAVRRAVEVTMQAYPMGQSHPFDV
                     KDDFAGVGHGLVFTDILDRLYQLTADKQYLAYAHWLYEEYSRQPLSQDDIQIANLLNP
                     NYRFKAHGVHTYEQLRSLLTAAVVADDAQLSSALTAALAKLESCLTPSGAPIGDEFIA
                     GREADSSETGYEYCSIHELLDTYTHLLQKTGDIRWADRAEWLFFNAAQGARHPSEPSI
                     AYLKTDNSFSMTGSLHPDDPQDEQNPQIRYKYSPVHQDVAVCCIPNAGRIVPYYIKAL
                     WLRSEDGFVKALYGASEFQAEINGVSIKITETTNFPIDLNVNFQVEVSEPVEFTLSFR
                     IPEWSTNFTLTGADEFSVRGNLIQVRKNW"
BASE COUNT          255 a          302 c          269 g          265 t
ORIGIN      
        1 agcctcattg tttcgtgttc tgcttggctg gtatgaagcc accagggatg aacgcacgct
       61 cactgctgtc cgccgtgcag tggaagtgac catgcaggcc taccctatgg ggcagtcaca
      121 tccctttgat gtgaaagacg atttcgctgg ggtcgggcat gggttggtgt tcactgacat
      181 tcttgaccgc ttgtaccaac tcaccgctga caaacagtac cttgcatatg cccactggct
      241 ttatgaggaa tacagccgcc agccgctcag ccaggatgat attcagatcg ccaacctcct
      301 caacccgaat tatcgcttca aagcccacgg cgttcacacc tatgaacagc tgcgctcttt
      361 gttgactgct gcagttgttg ctgatgacgc gcagttgagt tctgccctca ccgccgcgct
      421 cgccaaactg gaaagttgcc tcacgccatc aggtgctccg atcggtgatg agtttattgc
      481 agggcgcgaa gcagactcat ccgagacggg ctatgagtac tgttcgattc acgaattact
      541 cgatacatat acccacctgc ttcagaaaac tggcgacatt cgttgggcag accgcgctga
      601 atggctgttc ttcaacgccg cccagggcgc gcggcatcca tccgaaccgt ccattgccta
      661 cctgaaaacc gacaactcct tttcgatgac tggttcactg catcctgatg acccacagga
      721 cgagcaaaac ccgcagatcc gttacaagta ttcacccgta catcaggatg tggcggtatg
      781 ctgcattccc aatgcagggc ggatcgtgcc ctattacatc aaagcgcttt ggctgcgttc
      841 agaggatggt ttcgttaaag ctctgtatgg cgcatcagaa tttcaggccg agatcaacgg
      901 cgtaagtatc aagatcactg aaaccaccaa tttcccaatc gacctcaacg ttaactttca
      961 ggtggaagtt tctgaacctg tggaattcac gctcagcttt cgaatacctg aatggagtac
     1021 aaacttcact ttgactggcg cggatgaatt ttccgttcgg gggaatctga ttcaggtgag
     1081 aaaaaactgg c
//
LOCUS       DAOQLC010000455         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_126930_length_1091_cov_15.007722, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000455 DAOQLC010000000
VERSION     DAOQLC010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126930_length_1091_cov_15.007722"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PL000_23425"
     CDS             complement(<1..686)
                     /locus_tag="PL000_23425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91884.1"
                     /translation="MEYTLSLALVDFLPVTFTAIGLFYIVRMVAHIDSLRGKVAALGA
                     VLTVAGGFFKALWKLLMASSGGVIDINWMEDGLFVFMAPGYVLLAYSVWQTVRSVRGM
                     KTFHAWMTPAIFIALMFAGSIYLFTSNPASPSWERVLLSVMVLATVVTGVLLIVFGFR
                     QKMPMAGWLFILNLVGIFVLNGMARMPEQTIVLQWIEESINTTSWLAFMFAASKVYEY
                     TRVNFGVDTP"
     gene            complement(700..>1091)
                     /locus_tag="PL000_23430"
     CDS             complement(700..>1091)
                     /locus_tag="PL000_23430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91885.1"
                     /translation="ALRMTGSAFAQSKVWHIIICVLATALITLGSYIDILNLDLVFSP
                     EGGVTKYSNAFELMKGPPIPAVVTILVVLVFGVIMWRNAEWKWLFVGSLLMFLAAPMA
                     SMPLFQNVGEVAFAAGLVTTQIHAARK"
BASE COUNT          308 a          286 c          287 g          210 t
ORIGIN      
        1 atgggagtgt ccacgccaaa attgacgcgg gtgtattcat agactttcga agcggcaaac
       61 ataaatgcca gccatgaggt cgtgttgatg ctttcttcga tccattgcag gacgatggtc
      121 tgttcgggca tgcgagccat gccgttgagg acgaagatgc cgacgagatt gagaatgaaa
      181 agccaacccg ccatcggcat tttttggcgg aagccgaaca cgatcaacaa aacgcctgtg
      241 acgacggtgg caagcaccat cacgctgagt aagacgcgct cccatgaagg cgatgcaggg
      301 ttggacgtga agagatagat cgagcctgcg aacatcaatg caataaaaat ggcaggagtc
      361 atccaggcat gaaaggtctt catgccgcga accgagcgaa ccgtctgcca gacactgtat
      421 gccaggagca cgtacccagg cgccatgaaa acaaacaaac catcttccat ccagttgatg
      481 tcaatgacgc cgcccgaaga tgccatgagc agtttccata acgctttgaa aaatccgcct
      541 gccacggtga ggactgctcc aagtgcagcc actttgccgc gcaatgaatc aatatgcgcc
      601 accatgcgga cgatgtagaa caatccaatg gcggtgaagg ttacgggaag aaaatcgacg
      661 agggcgagcg agagggtata ttccataact acatcctttc tactttctgg cagcgtggat
      721 ctgagtcgtc accaacccag ccgcaaaagc gacctcgccg acgttttgga agagcggcat
      781 gcttgccatc ggcgcggcaa ggaacatcaa cagcgaaccg acgaagagcc acttccattc
      841 ggcattgcgc cacatgatga ctccaaatac tagcacaaca agaatggtca ccacagcagg
      901 gatgggcggt cccttcatga gttcaaacgc attcgagtat ttagtcacac cgccctcagg
      961 gctgaagaca aggtcaaggt ttaggatgtc aatataacta cccagcgtta tcaaagcagt
     1021 tgccagcacg caaatgatga tgtgccaaac tttgctttga gcgaacgctg accctgtcat
     1081 gcgcaaggcg c
//
LOCUS       DAOQLC010000456         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_127926_length_1087_cov_24.589147, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000456 DAOQLC010000000
VERSION     DAOQLC010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127926_length_1087_cov_24.589147"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(279..539)
                     /locus_tag="PL000_23435"
     CDS             complement(279..539)
                     /locus_tag="PL000_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNC91886.1"
                     /translation="MDVIKVSALSRTSAVAGAIAGVIREHKRAEVQAIGAGAVNQAVK
                     AWTLATGYLKLDGIDVVCTPEFVEVNIDEKVRTAVKFTIEAR"
BASE COUNT          314 a          283 c          248 g          242 t
ORIGIN      
        1 ccgcaacaac ggcgcggcgc tgacagcatc tccctgcatg gcaggcgcag aaacaaaagc
       61 cagcaggctc accagcacgg caatgaaaac aaacaaacgc gaaacacgga aagtattttg
      121 catgaaaaag aaaaggggag aatcaacccg caacggcagc ccagcacaag gcaagtccgc
      181 gcggagaaac gtccgcaaga agattatacg ccaccctgca ttttattgtc tatgaaagag
      241 gtgacaagcc cgcagatcga gcctgtcacc tctttgtact aacgtgcttc aatcgtgaac
      301 ttcacagcgg tacgaacttt ttcatcgatg ttcacctcaa cgaactcagg tgtgcagacc
      361 acatcgatac catcgagttt gagataccct gtggccaatg tccacgcctt tacggcctga
      421 ttcacggccc cagctccgat ggcttgcact tctgcccgtt tatgttcgcg gatcacgccc
      481 gcgatcgccc cggcgactgc agaggtacgg gagagagcag aaactttgat aacatccatg
      541 gcaacctccc aaggttggct aagaagggaa cggacttaca ggtgcattgt acatggttca
      601 cttaacagat tcaagtaata tgtaaatatt tatatggaat cgtaatagta gtagagcctg
      661 tggatactgt ggatagaatc gactcaaccc tttatatggc atgactcaaa aacgaatcac
      721 ccgtatctgg ggtctgatcc tgctttcaaa gaatccacac cctgtggata gattctggat
      781 aactcaacca ccaaaaagag gtctgtggag aactttcaac gggttttcca tattttggga
      841 gtattgaaaa tcacccccca tatatggggg tgacgatttt taagttatcc agcccatccc
      901 ggtaaaatag cacacctttt ccacagtcag gggcagttct ccacagtttt ccgccactta
      961 tccacagatt ttttagcctg cggaaaatgt tgtctgaaca atacccccta aatgtgggcg
     1021 gcagatcggg tatttcggat agcaaatgga aagttacccc tcacccccaa caaaaaaggc
     1081 ggacccg
//
LOCUS       DAOQLC010000457         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_128883_length_1083_cov_29.599222, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000457 DAOQLC010000000
VERSION     DAOQLC010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128883_length_1083_cov_29.599222"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..358)
                     /locus_tag="PL000_23440"
     CDS             complement(23..358)
                     /locus_tag="PL000_23440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91887.1"
                     /translation="MKNYFPNNPAGLASAQAIADPKTILVFPTQIVVLTGTDYQPDPT
                     VDPDITAANGDAILQQLINASQAQLTTWIATHVNLPVTNLTQAATAIQALQSVVSSLI
                     LAMKAALKK"
     gene            complement(362..>1083)
                     /locus_tag="PL000_23445"
     CDS             complement(362..>1083)
                     /locus_tag="PL000_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91888.1"
                     /translation="LKKWSIANFKTWLQPLLYGWGFPRRICAVTTPWLYCPSSTVVAG
                     GAGAAYITLGTALPAAINVAGKCYVPAVGSMSAGWYDFSSCTTTQVNLVAAVVTGGAF
                     TATVGAYATGPSYTLPANSLGPNGRIDTDLWIMHNNTAGLKYSNLLFGATSIWANSFS
                     TNTIDSIRFSTVNRSNTGKQLTGINGSGTYFGSVSSATTLSETSVDTTNSITVSMALK
                     IATATDLIVLMGWSQTLTYGA"
BASE COUNT          295 a          254 c          262 g          272 t
ORIGIN      
        1 ttgtccatag atattcccag acttacttct tcaatgctgc cttcatagca agaattaaag
       61 aggagacaac agattgcaat gcctgaatcg ctgttgccgc ctgagtcaag ttggttacgg
      121 gcagatttac atgggtggca atccaggttg taagctgcgc ctgagatgca ttgatcagtt
      181 gttgcaagat cgcatccccg tttgccgcag taatatcggg gtcaaccgta gggtcgggtt
      241 ggtaatcagt accagtcagg accacgattt gagtaggaaa aaccaaaatt gttttcggat
      301 cggctattgc ctgggcgcta gccaatccag cagggttgtt aggaaaataa tttttcatgg
      361 tttacgctcc atatgttaag gtctgactcc agcccatcaa aactatcaaa tcagtcgccg
      421 tggcaatttt caaagccata ctaacggtta tgctgttagt agtatcgaca ctggtttctg
      481 acaaagtagt ggccgatgat acagatccga aatatgtacc ggacccgtta atgcctgtta
      541 gttgtttgcc agtattgctt ctgttgactg tgctaaaccg gatggaatca atcgtattgg
      601 tactaaaaga gttggcccaa attgacgtag caccaaataa aaggtttgaa tatttcagac
      661 cggcagtatt gttgtgcata atccaaaggt cggtatcgat acggccgttc ggacctaaag
      721 aattagcagg caaagtataa ctcggcccgg tcgcatacgc cccgacagtt gctgtaaaag
      781 cacctcccgt aactacagca gcaaccaaat tgacttgtgt tgtcgtacag ctagagaaat
      841 cgtaccaacc tgcggacatg ctgccaacgg cagggacgta acattttccc gccacgttga
      901 ttgccgctgg caaagcggtt ccaagagtga tgtaagcggc ccctgccccg ccggccacca
      961 ctgtactaga cgggcaatac aaccaaggcg tggtaacagc gcaaatacgg cgagggaaac
     1021 cccatccgta caataatggc tgaagccacg ttttgaaatt ggcaatgctc cattttttca
     1081 gca
//
LOCUS       DAOQLC010000458         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_130118_length_1078_cov_26.631476, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000458 DAOQLC010000000
VERSION     DAOQLC010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130118_length_1078_cov_26.631476"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PL000_23450"
     CDS             <1..1039
                     /locus_tag="PL000_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91889.1"
                     /translation="KEIPMRVTRESLLRIAKETAQERAYNDKDIVAAYLTGSLLNVEP
                     MLGGTADIDIVFVHRSQPKQLREFVKLTPDFHLDISRRMKEEFKSPRELRGDPWLGYE
                     MYDPILLYEREKFFDFVQASLRAGFDFEQPPLMLQRCRKMLSHGRGIWMDLTEVDESS
                     VGPTEVRKYMKALFHALNAIVELSGPPIHERRLLLEFPARAEAAQQTSMVAGAFALLG
                     ANAVDAEKVKSWVADWKVAYSLASEKDGVDLRINPIRLNYYEKAIKAMLEGSIPLAAL
                     WPLFHTWTMAAEVLDGDHLKFWQTAAGELGLLGAGFVEKVDGLDHFIDDIEVVFDGIA
                     RENGLDVSTGL"
BASE COUNT          258 a          293 c          281 g          246 t
ORIGIN      
        1 aaaggaaatc cctatgcgcg taacacgtga atcacttctt cgaatcgcaa aagagaccgc
       61 ccaggaacgg gcctacaacg acaaggacat tgtcgccgcc tacctgacag gctccctgct
      121 caatgttgaa cccatgcttg gtggcacggc tgatattgac attgtcttcg ttcacagatc
      181 ccagccgaaa caattgcgcg agttcgtaaa actgacacct gattttcatc tcgatatcag
      241 ccgtcgaatg aaagaagagt tcaaatctcc gcgtgaattg cgcggcgacc cgtggctcgg
      301 ctacgaaatg tacgacccca ttttgttgta tgagcgcgaa aagttcttcg attttgtgca
      361 agccagtctg cgcgcaggtt ttgacttcga acaaccaccg ctcatgctgc aacgctgccg
      421 caaaatgctc tctcacggac gcggcatctg gatggacttg accgaagtgg acgaatcctc
      481 ggtcggcccg acggaagttc gcaaatatat gaaggcgctt ttccacgcct tgaacgcaat
      541 cgtcgaatta agcggacctc ccatccacga gcgcaggctt ttgctggaat tccccgcccg
      601 cgccgaagcc gcccaacaga caagcatggt ggctggcgca tttgccctgc tcggtgccaa
      661 tgcggtcgat gctgaaaaag taaaaagctg ggtcgccgat tggaaagttg cctattccct
      721 cgccagtgaa aaagatggcg tggacttgcg catcaatccc atccgcttga actattacga
      781 gaaagccatc aaggccatgc tcgaaggttc gattcccctc gcggcgctct ggccgctctt
      841 ccacacctgg acaatggctg cggaagtgct ggatggcgat catctcaaat tctggcaaac
      901 tgcggcaggt gagttgggcc tgctcggtgc tggctttgta gaaaaagtgg atggccttga
      961 ccatttcatt gacgacatcg aggtcgtgtt cgatggcatt gcccgcgaaa acgggctgga
     1021 cgtatcaacg ggattgtaaa tttcaaactt ccgaagtctt aaggcttcgg aagttttt
//
LOCUS       DAOQLC010000459         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_130119_length_1078_cov_19.860215, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000459 DAOQLC010000000
VERSION     DAOQLC010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130119_length_1078_cov_19.860215"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PL000_23455"
     CDS             <1..494
                     /locus_tag="PL000_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91890.1"
                     /translation="QMKLDQMTAEELKMLGIERAEMEMPAIPEELKDLPLTKASKEFL
                     DAIETKNRVIEIGGPDSYVLQHLDSISAEASVFITEPLEPDIILLRENPSKIAALEEF
                     LHGTQRKLDNLKDIDSVILEMHVKDFMIRHRRMLGLSDSDVQMLEALKVEELKRAKMR
                     GYK"
     gene            502..900
                     /locus_tag="PL000_23460"
     CDS             502..900
                     /locus_tag="PL000_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91891.1"
                     /translation="MNDSNYQLFFRVVDHRVIRGNLILVSDRTDSGQARTLEIIQVKD
                     PKGGNAKMYTAGSVSNWQMPGAKPAPFCMAIKGIKPEEIPIGSTVWLESREITEKELK
                     LYRKLAKSPAPIPGGLPEVSQPKGGKKQAV"
BASE COUNT          350 a          196 c          287 g          245 t
ORIGIN      
        1 atcaaatgaa gctcgatcag atgacagccg aagagcttaa aatgcttggc atagaaagag
       61 cagagatgga gatgccagcc ataccagagg aattaaagga cttgccgctt accaaagcat
      121 ctaaagagtt tttggatgcg atagaaacga agaatagggt catagagatt ggtgggccag
      181 attcttatgt gctgcaacac ctggactcaa tatcggctga ggcttcggta tttattactg
      241 aaccattaga accagacata atcttgttgc gtgagaaccc aagtaagata gctgctctag
      301 aggaattttt gcatggaacg cagagaaagt tagataacct gaaagacata gacagtgtta
      361 ttttggagat gcatgtaaag gatttcatga ttcgtcaccg tagaatgtta ggtctcagtg
      421 atagtgatgt acagatgctg gaagctctga aagtagagga attgaagcgg gcaaagatgc
      481 gaggctacaa gtaggtaaga catgaatgat tcaaactatc aacttttctt tcgggtcgtt
      541 gaccatcgag taattagagg caatttgata ctggtttcag atagaactga ttctggacaa
      601 gcaagaactc ttgagattat tcaggtaaaa gaccccaaag gtggaaatgc aaaaatgtat
      661 accgctggca gcgtcagtaa ctggcaaatg ccaggtgcga agccagcgcc tttttgcatg
      721 gcaatcaagg gaatcaagcc tgaggaaata cccataggca gtacggtttg gttagagagt
      781 cgagaaatca cggagaaaga attgaagctg tacagaaaac ttgccaagtc accggctcct
      841 attccaggtg ggttacccga ggtgtcgcag ccgaagggcg gtaagaaaca ggcggtttag
      901 ttatcggtcg caggtgaggc gggatgggat ggtttgagta ccccaaatgg gggctagcat
      961 ataaatgaca accgggggcg gtggtacatg ccaagcgctg aaaataaata aaagccccga
     1021 aatacccatc cagtaagcaa tacaagccac gcatatgtgg taaagccaca taatatat
//
LOCUS       DAOQLC010000460         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_136184_length_1054_cov_51.920921, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000460 DAOQLC010000000
VERSION     DAOQLC010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136184_length_1054_cov_51.920921"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PL000_23465"
     CDS             complement(<1..956)
                     /locus_tag="PL000_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041205629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNC91892.1"
                     /translation="MKHYKQLTREQRYQISGLKKAGLNQSQIATEVGVHKSTISREFE
                     RNQGRRGWRPKQAQELRDERRKKCVNAQRFSLPEWAEIERLIRLDMSPEQASCRLALE
                     CGLKISHETIYMHIYEDKRRGGGLWQHLRCQKPRRKRYAGGQERRGAIKNRIGIDERP
                     GIVDQKSRIGDWEGDTVIGKNHRGALVTLAERKSRYILAAQVPGKQASGVTAAIMRLL
                     RPHKDKCYTMTFDNGKEFAEHETIAAELDADVYFAHPSHSWERGLNENSNGLLRQYFP
                     KAMELIEVTQEQVQWAVDRLNHRPRKVLGFRTPFEIFFGKTVR"
BASE COUNT          198 a          319 c          246 g          291 t
ORIGIN      
        1 cgcaccgtct tcccgaagaa tatctcaaac ggggttctga atccaagcac tttgcgtggc
       61 cggtggttga gtctatccac cgcccattgc acttgttcct gggtaacctc aatcagctcc
      121 atcgccttgg ggaaatactg gcgaagtagc ccattgctgt tctcattcaa accacgctcc
      181 catgaatggc tgggatgggc gaaatagaca tccgcatcca gttccgccgc gatggtttca
      241 tgctctgcaa actcttttcc gttgtcgaat gtcatggtgt agcacttgtc tttatgggga
      301 cgcagtagcc gcattatcgc cgccgtcaca cccgatgcct gtttgccggg tacttgggct
      361 gccagtatgt agcgcgactt ccgttcagcc aatgtgacca atgcgcctcg atggttcttg
      421 ccgatcaccg tgtcgccttc ccagtcacct atgcggctct tctgatcgac aatccccggg
      481 cgttcgtcaa tgccgatccg gttcttgatc gcgccccggc gctcttgacc acccgcgtaa
      541 cgttttctgc gcggcttctg gcagcgcaaa tgttgccaca ggccgccgcc acggcgtttg
      601 tcttcataaa tgtgcatgta gatcgtctcg tggctgattt tcaatccgca ttccagtgca
      661 agccggcaag atgcttgttc cggactcatg tccaaccgga tcaaccgttc aatctccgcc
      721 cattccggca atgagaaacg ctgggcattg acgcatttct ttcgacgctc atcccgcaat
      781 tcctgcgcct gctttggacg ccagccacgc cggccttggt ttcgttcaaa ttcccgagat
      841 atcgttgact tatgtacacc cacttcagtc gcaatttgcg attggttcaa tcccgctttc
      901 ttcaaaccag aaatctggta tcgttgctct cgggtgagct gcttgtagtg cttcatctgt
      961 gctcctttta cttggtcgtt taagcagctc cgatactacc gcagcttgcc tataaacttc
     1021 cttacaaaag ttgcacttca atgttgaatc cgcc
//
LOCUS       DAOQLC010000461         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_136187_length_1054_cov_17.461461, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000461 DAOQLC010000000
VERSION     DAOQLC010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136187_length_1054_cov_17.461461"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PL000_23470"
     CDS             complement(<1..200)
                     /locus_tag="PL000_23470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91893.1"
                     /translation="MKIESRNNLYKELDHIWQRLIDAIKDQEPILLEGKERTFFLAYW
                     LYGEVGNGNFDQFYYNSTGDYSL"
     gene            complement(385..>1054)
                     /locus_tag="PL000_23475"
     CDS             complement(385..>1054)
                     /locus_tag="PL000_23475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC91894.1"
                     /translation="SYGDRLILDEFSASVPPGSIVGIVGPNGAGKTTLLKMITGQEKP
                     DSGTVKIGETVKLSYVDQNRTLDPEKTVYEEISQGADFLELGKRKVNSRAYCASFNFQ
                     GSDQQKKVGILSGGERNRVHLAKTLTEGANVILLDEPTNDLDVNTLRALEEAIEEFAG
                     VALVVSHDRWFLDRICTHIIVYEGDSVAKMYIGNWSDYETAMMEKFGKDLTPHRVKYR
                     TLKR"
BASE COUNT          221 a          247 c          303 g          283 t
ORIGIN      
        1 agtgaataat cgcctgtgga gttgtaataa aactggtcga aattaccatt gccgacctcc
       61 ccatacaacc aataagccag gaaaaaagtt ctttctttcc cttccaacag aattggctcc
      121 tgatctttaa tcgcatcgat gagtctttgc caaatatgat ccaattcttt atacaggtta
      181 tttctagact caattttcat aagtagatcc aaggatattg agtatctatc gttttagggt
      241 acggtatttc acccgatgcg gaataaatcc ttgccgaatt ttcttccatt atgatttcat
      301 aatcgatcgt aggggcgacg tctcgtcgcc caaggatgat ggtgcaaaca aaaaggggcg
      361 gggggacccc gcccctacgg acgtttaccg tttcagcgtt ctatatttca cccgatgcgg
      421 agttaagtct ttcccgaatt tttccatcat cgcggtttcg taatcggacc agttgccgat
      481 gtacatcttg gcgacggagt cgccttcgta gacgatgatg tgggtgcaga tgcggtcgag
      541 gaaccagcgg tcgtggctga cgacgagggc gacgcctgca aattcttcga tggcttcttc
      601 gagggcgcgc agggtgttga cgtcgaggtc gttggtgggt tcgtcgagca ggatgacgtt
      661 ggcgccttcg gtgagggtct tggcgagatg gacgcggttg cgttcaccgc ctgagaggat
      721 gccgactttc ttttgctggt cggagccttg gaagttgaac gaggcgcagt aggcgcgtga
      781 gttgaccttg cgcttgccga gttcgaggaa gtcggcgcct tgcgaaattt cttcgtagac
      841 ggtcttctcg gggtcgaggg tgcggttctg gtccacgtac gagagtttga cggtctcgcc
      901 gatcttgacc gtgccgctgt cgggtttttc ttgtcctgtg atcatcttga gcagggtggt
      961 cttgcctgcg ccgttgggac ccactatccc gacgatagag cctgggggaa cagaagcaga
     1021 aaactcgtca agaatgaggc ggtcaccgta agac
//
LOCUS       DAOQLC010000462         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_138024_length_1047_cov_16.442540, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000462 DAOQLC010000000
VERSION     DAOQLC010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138024_length_1047_cov_16.442540"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..362
                     /locus_tag="PL000_23480"
     CDS             24..362
                     /locus_tag="PL000_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91895.1"
                     /translation="MSQVFVWVGAVLAILYGALTSFAGFGQSKQDKIQPWAAWGMVLC
                     GGLVVSSGVLALFASGSSPSMLVIGLIGIHVLAINNGLKMFGKITFSHHLVRLVISIG
                     IIALNQLGMK"
     gene            377..>1047
                     /locus_tag="PL000_23485"
     CDS             377..>1047
                     /locus_tag="PL000_23485"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNC91896.1"
                     /translation="MQDKLNEIEKSALAALAEVNDPAAVEAWRVTHLGRSSELMGVFS
                     GLGKLSKEERPAVGQAANRVKVALEAALEEKAAVVKQAALAKSLAEEKLDVTLPGRNK
                     HVGRLHPSTQQLRRVLSILAEMGFQVFTSREVETDEFNFQHLNFPPHHPARDMQDTFF
                     VEAENRGDNPILMRTHTSPGQIHAMRYFAATDPNNPPPIRIALPGMCFRYEQITARSE
                     VQFNQV"
BASE COUNT          239 a          250 c          302 g          256 t
ORIGIN      
        1 ttttgtttgt ataggaggac gatatgagtc aggtttttgt ttgggtcggc gctgtattgg
       61 cgatcctcta cggagcattg acgtcctttg cagggttcgg tcagtccaaa caggataaga
      121 ttcaaccctg ggcagcatgg ggcatggtct tgtgtggcgg cctggttgtg tcctctggag
      181 tgctggcgtt attcgcatca ggctcatcgc catcgatgct ggtcattggt ttgattggca
      241 ttcacgtttt ggcaatcaac aatggcttga agatgtttgg aaagatcacc ttttctcatc
      301 atcttgtgag actggtcata tcgatcggaa taattgcatt gaaccagtta ggaatgaaat
      361 aaattcggag gcatgtatgc aagacaaatt gaacgagatc gaaaaatccg cgctggcagc
      421 gctggcagag gtcaatgacc cagctgcagt ggaagcctgg cgtgtgactc acctcgggcg
      481 aagctcggaa ttgatgggag tcttttccgg cttgggcaaa ctttccaaag aggaacgacc
      541 cgctgtcggt caggctgcga accgtgtgaa ggtggcgctt gaagctgcgt tggaagaaaa
      601 agccgcagtg gtgaaacaag ccgcgttggc gaaatccctc gccgaagaaa aattggatgt
      661 gactctgccc gggcgtaaca aacacgttgg gcgtttgcat ccatccacgc agcagttgcg
      721 ccgcgtgttg agcattttgg cggagatggg ttttcaggtg ttcacctcgc gcgaagtgga
      781 aacggacgag ttcaactttc agcatttgaa cttcccgccg catcaccccg cgcgcgatat
      841 gcaagacacg ttttttgtag aagcggaaaa ccgcggcgac aatcccattt tgatgcgcac
      901 gcatacctcg ccgggacaaa tccacgcgat gcgttacttc gcagcgaccg acccaaacaa
      961 cccgccgcct attcgtatcg cgttgccggg tatgtgcttc cgctatgagc agatcacggc
     1021 acggtctgaa gtgcagttca atcaggt
//
LOCUS       DAOQLC010000463         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_140812_length_1037_cov_13.489817, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000463 DAOQLC010000000
VERSION     DAOQLC010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140812_length_1037_cov_13.489817"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..814)
                     /locus_tag="PL000_23490"
     CDS             complement(152..814)
                     /locus_tag="PL000_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91897.1"
                     /translation="MRFVFAIASCTTLILQGCALFAKPMEKPVIEEKLNRSLTSSASI
                     GTLSLTPERRVVLVNFANNRFCAEAPTETGIDMASLSDLVAKFNSGKADTPSGSAGIT
                     TALTSQNMAINKRTQGMQLFLANSYLACQMYMNGGIDQAQLLEMQFQTLKIVSPLIEK
                     EIPYFYAKEARPIAEEKSAASGSDTLDKSIRGSAKTTVEKILDTRNAKPDEAPKPSPD
                     GK"
BASE COUNT          211 a          199 c          303 g          324 t
ORIGIN      
        1 gagcgacatc gagctgatgc gcctggcgat gcggaggaag tgggtggagt agggggtgac
       61 tgttctggta tgaatgatgt tgcgttcttc atgccatggt ttgttgaagt actacacagc
      121 gaccccttcg ttcaatgagg atcagttgca gctatttccc gtccggggat ggtttgggcg
      181 cctcgtctgg ttttgcattg cgtgtgtcga gtattttttc tactgtggtt tttgccgagc
      241 ctctaatcga tttgtctagg gtgtcagatc cactggcggc ggatttttct tctgctatgg
      301 gtcttgcttc cttggcgtaa aagtatggaa tctccttttc gattaggggg gaaacaattt
      361 ttaaagtctg aaattgcatt tcaagaagct gggcttggtc gattcctccg ttcatataca
      421 tttggcaggc aaggtaagag ttggcaagaa atagttgcat gccttgtgtg cgcttattga
      481 ttgccatgtt ttggcttgtg agggcggtgg ttatacccgc tgagccagag ggtgtgtcgg
      541 ctttgccgga attgaatttt gcgacaagat cgctgaggct tgccatgtca attcccgttt
      601 ctgttggcgc ctctgcgcag aatcggttgt tcgcaaagtt tacaagaact acgcgacgct
      661 ctggtgtgag cgataatgtt ccaatgcttg cgcttgatgt taaggatcga ttgagttttt
      721 cttcaattac tggtttttcc atgggctttg caaaaagagc acatccctga agtatgagtg
      781 tggtgcagct tgcaatagca aaaacgaatc gcatggttgt actccgtttg tgggggggcg
      841 attaatgact ttgtactgtt tttgaagata tattatggtg ctaaatatgc aagatatttt
      901 tgaaaatttt ttagtggact ggctctatgc tgagtgaggt tgatgcttct atttgagatg
      961 ccaccgcagt ccgcagacga ggccggcgag catggtcagc acgcccaggt gatggcagac
     1021 gcggccccag cggtggg
//
LOCUS       DAOQLC010000464         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_142514_length_1031_cov_23.194672, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000464 DAOQLC010000000
VERSION     DAOQLC010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142514_length_1031_cov_23.194672"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PL000_23495"
     CDS             complement(<1..869)
                     /locus_tag="PL000_23495"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HNC91898.1"
                     /translation="MTPSLEIRVLGKPQLNWQGQPLTADLISAKGQALLFYLAVTGQV
                     CSRQFIAGLLWGDFSEERARGNLRLTLSKLRSVIGDYIIATRQSLAFDFSLPHSLDAS
                     EFIRHGAAPQKSATSELEAVLALYRGNFLDDFYLHEAPDFETWVVVERERFQQMAMAL
                     LAHLAASAQQQNDFEKAADFTRRILALEPWREEMHRQLMTVLAQSGQRSAALSQFEAC
                     KKHLDEELGVEPSAETKALYDRIKQGDVKEADGESARRVESKSPSKSSPRKPASDLRH
                     NLPQTLTPFLGREA"
BASE COUNT          247 a          259 c          282 g          243 t
ORIGIN      
        1 gcttcacgcc caagaaaagg cgtcagggtt tgaggcaggt tatggcgtag gtctgatgcg
       61 ggttttctcg gggaggattt ggatggggat tttgattcaa ctctgcgggc ggattctcca
      121 tccgcttctt tgacatcccc ctgcttgatc ctgtcataaa gagccttggt ctccgctgac
      181 ggctcgacgc ccaactcttc atccagatgt tttttgcaag cttcgaactg actcagcgcc
      241 gcgctgcgtt gaccgctctg cgcgagcacg gtcatgagtt ggcgatgcat ctcttcgcgc
      301 cacggctcga gtgcaaggat gcggcgggtg aagtcagcgg ctttttcaaa atcgttttgc
      361 tgttgtgcgg acgcagcaag atgggcaagc aacgccattg ccatctgctg aaaacgctcg
      421 cgttcgacca cgacccaggt ttcaaaatcg ggcgcttcgt gcaggtaaaa atcgtccaga
      481 aaatttccgc gatagagtgc gagtactgct tcgagttcgc tggttgcgct tttctgaggc
      541 gcagcaccgt gacggatgaa ctcacttgca tcgagcgagt gaggcagtga aaaatcgaaa
      601 gccagcgatt ggcgggtggc aatgatgtaa tcgccgatga cactgcgaag tttgctgagt
      661 gtgagtcgta aattcccccg cgcgcgttcc tccgaaaaat caccccataa caaacctgcg
      721 atgaactgtc gcgaacaaac ctgccctgtt acagccagat agaagagcag cgcctgccct
      781 ttggcagaga tgaggtcggc agtgagcggc tgtccctgcc agttgagctg cggttttccc
      841 aaaacacgaa tttcaagcga tggggtcaaa cttgaattcc tccaattttg gacgaatttg
      901 acaaactaac ggtaaataaa cgctggaaaa actgcgccat cataagattt ggttatcgag
      961 ttcgaccaag ttcagatgac aaggagatta tatccaatgc cacagatttt caaagagcga
     1021 atgacgaccg a
//
LOCUS       DAOQLC010000465         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_143363_length_1028_cov_18.417266, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000465 DAOQLC010000000
VERSION     DAOQLC010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143363_length_1028_cov_18.417266"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..984)
                     /locus_tag="PL000_23500"
     CDS             complement(121..984)
                     /locus_tag="PL000_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase GES-2"
                     /protein_id="HNC91899.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIV
                     DPQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVEWSPA
                     TERFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRK
                     EPEMNDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRA
                     GFPKDWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQ
                     VITQLILSTDK"
BASE COUNT          224 a          284 c          266 g          254 t
ORIGIN      
        1 agcataacgc ctgagttaag ccgcggtgcg cagtggcaag cggcgtgcga agcttagcgt
       61 agcacgcaag cgccgccacg aagcaacgtc ggcttgaacg aattgttaga cgggcgtcaa
      121 ctatttgtcc gtgctcagga tgagttgtgt aataacttga ccgacagagg caactaattc
      181 gtcacgttct acggccgata gtttcggggc cgttgtatac accgctacag cgtaatctct
      241 ctcctgggct ttaaaaaaac caatgtcgtt ccggcccccg ttggcgcagg taccagtttt
      301 ctctccaaca acccaatctt taggaaaacc cgctcgtagt gtcgcgtctc ccgtttggtt
      361 tccgatcagc cacctctcaa tggtgtgggt cgaggtggac gtcagtgcgc cgccatagag
      421 gactttagcc acagtacgtg ccatagcaat aggcgtagtt gtatctctga ggtcgccagg
      481 tgtgttgtcg ttcatctccg gctctttccg gtctagccga ctcacagagt cgccaatttt
      541 acgaaaatac tgcgtcattg cagcaggtcc gccaatttct ctcagtaaga ggttagtagc
      601 cccattgtcg ctaagctgca ccgcagcttg cgctgcctcg agaaccgtca tgtgtcccga
      661 tgctagaaac cgctccgtgg caggagacca ttcgacgatc atgtccggcc catatgaaag
      721 ttttcgatcc ccccgctcgg tgcctgagtc aattctttca aagaccagcg cggctagcgg
      781 aaacttgaac gttgagcaca ttgcaaaacg ctgcgccatt cggtggcccg cgacgatctc
      841 tccttgggga tcgacgatcg caacaccgat ctgagctgct ttttcgcgct ctagcttctc
      901 aagatcggtc ttgaaggtta atttttccga cgcatatgca gagtgagcga tccctgccag
      961 taatagtgcg tgaatgaagc gcatggtgat cccttgagat ggaaattatc tttgtacgcc
     1021 cgtctaac
//
LOCUS       DAOQLC010000466         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_143644_length_1027_cov_26.199588, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000466 DAOQLC010000000
VERSION     DAOQLC010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143644_length_1027_cov_26.199588"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PL000_23505"
     CDS             <1..1025
                     /locus_tag="PL000_23505"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HNC91900.1"
                     /translation="RAGSCTTARATATPSRWIGTRRAMRLETMCGMDYAGIVPTTVGT
                     GDRQAPGSGRTSAAGKQPGAFFTSRIPFYDGAIGMASAMLVSHAGSWRTLSHRRQLDR
                     QARGDGSSLHGRIVMSSILPIEQILVYGNMPSAVFYKTPAWRKVRYEALTIFGNRCQL
                     CGSGPQKAQLHVDHIKPRFLYPESCLDIQNLQVLCEDCHIAKGVEYVDDCRNKITQLP
                     AFEMRDILRLERWCLLLTMRPPRTKVEVEYWGDGVRSSNKKYRQRWRAFVRFCFIEKL
                     NYDEARVMRLRDFLQTSQAYSLKFSKFTFGKKPEDFVFDIGGCLFPPSMYTLLADDQG
                     CSGDRS"
BASE COUNT          209 a          280 c          287 g          251 t
ORIGIN      
        1 gccgcgctgg atcgtgtacg accgcacggg cgacagctac cccgtcgcgc tggattggta
       61 cacggcgcgc gatgaggctt gaaactatgt gcggcatgga ctatgctgga atcgtcccta
      121 caacagtggg gaccggggac cgccaagctc cgggtagtgg gcgcaccagc gccgccggca
      181 aacaaccggg cgcttttttt acgtctagaa ttcccttcta tgacggtgcg atagggatgg
      241 cttcggccat gctggtctcc catgccggtt cttggcggac cctgtcgcac cgtcgccagc
      301 ttgaccgcca agcacgcggc gacggttctt cgcttcatgg gagaatcgtt atgtccagta
      361 tcctgcctat tgagcagatt ctcgtttacg gcaatatgcc atccgccgtt ttctataaaa
      421 cgccagcatg gcgcaaagtc cgctacgaag ctcttacgat ttttggcaac cgctgccagc
      481 tttgcgggtc cggcccgcaa aaagcccagt tgcacgtcga tcatatcaag ccccggtttc
      541 tttaccctga gtcctgtctg gatattcaaa accttcaggt gctgtgcgaa gactgtcata
      601 tcgcaaaggg ggttgagtac gtcgatgact gccgcaacaa aataacccag ttgcccgctt
      661 ttgagatgcg cgacattttg cgcttggagc gctggtgttt attgctgacc atgcgtcctc
      721 cccgcaccaa ggttgaggtt gagtattggg gtgatggcgt caggtcatcc aataaaaaat
      781 accgccagcg ctggcgggcg tttgtccgct tctgttttat tgaaaagctg aactatgacg
      841 aggcccgcgt tatgcgcttg cgggattttc tgcaaacatc acaggcgtat agcctaaagt
      901 tttccaagtt tacgtttggc aaaaagccgg aagattttgt ttttgatatt ggcggctgcc
      961 tgttcccgcc cagcatgtac acattgctgg ccgacgacca aggatgctcg ggagatcggt
     1021 catgagc
//
LOCUS       DAOQLC010000467         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_145080_length_1022_cov_16.797311, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000467 DAOQLC010000000
VERSION     DAOQLC010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145080_length_1022_cov_16.797311"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PL000_23510"
     CDS             <1..351
                     /locus_tag="PL000_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91901.1"
                     /translation="LVGKSGAEEMSFECDDPGLLPGNLRDLQRLAVLVMAWCPDSFTQ
                     AGLQSALDEWNRWVLTPVQDEGTLGQGTRANRINWFCSNLAKWSKDKYGTWKAAAEAL
                     ACDEKTLRSDAKAK"
BASE COUNT          242 a          269 c          290 g          221 t
ORIGIN      
        1 cttgtcggga aaagtggggc ggaggaaatg tcattcgagt gcgacgaccc tggcctgttg
       61 ccaggcaatc ttcgtgacct tcagcgcttg gctgtacttg tgatggcatg gtgtcctgat
      121 agctttacgc aagcaggcct acaaagtgcg ctggatgaat ggaataggtg ggttctcact
      181 cccgtacaag atgagggcac cttagggcaa ggaaccagag caaatcggat caactggttt
      241 tgctcaaact tggcgaagtg gtcgaaggat aaatatggta cttggaaggc agcggcagag
      301 gcgctagctt gtgatgagaa aacgttgagg tccgacgcaa aagcaaagtg aagaatcacc
      361 tcttatttcg agagcgcagg ctgcttttgt cacctctgcc actcaaactg aataaggtga
      421 atggcagctt tggccgacca actgcttgtt gccactggcg tgaggccgga tgtcggcctt
      481 tccggccgtt ggccagtccc tcgattcatg gcagctgtaa ggtggtaagc catcggaagg
      541 tcgacgacag caattcaggc gaaaatcata aatatgctga tgccatccag atttcattcg
      601 gcaagggatg caaagtgatt caaggagccc gcgctctacc tgttcaacat ctcacgattc
      661 gcgtgccttg gcacgactcc ggctggaatg gggccatctg ccaagtctgc actgcgaaca
      721 cgtgctgcat cgttctgcca cgaatcgcga ccgggcggga cgacgcattt gaaatgctgc
      781 atgccggcga gagtatcgag gcactagatc gatccctgca ccccccctgt gtcgatgagc
      841 atgggacctt catggcgaag ttcccactct caatgcagaa gaggcaccct tacgcggaga
      901 gtgccaaaac tacccacggg cactttgccg aaactccgta caccattcgc ccatactcag
      961 ccgctggctg aagttgcccc catcccgtgg acaggtcatg gtttgaaact caagcgctcg
     1021 ga
//
LOCUS       DAOQLC010000468         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_146254_length_1018_cov_16.483904, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000468 DAOQLC010000000
VERSION     DAOQLC010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146254_length_1018_cov_16.483904"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..990
                     /locus_tag="PL000_23515"
     CDS             88..990
                     /locus_tag="PL000_23515"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNC91902.1"
                     /translation="MSSIPMKEVMLVVYFIVDEWYQEEGHRLLKGKPGVKPIFSDSEV
                     ITLMLVQDFIPYPGENQYIGYLRANYLALFPQLLTQSQYNRRARSLRLLVEAFRRHWL
                     DELGLLMNQDLLLDTKPVPVMGYKRSKKHSAFAGSAGYGVCVSRNLKYYGYKLVTLST
                     LEGIPLVYELVAANLDERVAAQAVVYCVRGCRIYGDKGFLGEEWQHQLFQHTRNRLFT
                     AKRSNQTTQNPSEFDRWLNSLRERIEGLFNEVQNTGRNLERLLAKTVLGLTTRVIAKM
                     TSHLVKLILRKCYGIDVQTFTCTA"
BASE COUNT          272 a          255 c          261 g          230 t
ORIGIN      
        1 acggcttatg tgaattcaaa aggttgacaa agcggggaat attctccacg attatggtag
       61 ccaaacccca atcaggagaa tattcccatg tcaagtatac ccatgaaaga agtaatgttg
      121 gtcgtctatt ttatcgtgga cgagtggtat caagaggaag gacaccggct actcaagggc
      181 aaacccggtg tgaaacctat cttcagtgac agtgaagtga tcactttgat gcttgtccaa
      241 gactttatcc cgtatccggg cgaaaaccag tacattggct acctgcgggc caattacctg
      301 gctttgttcc cgcaattact gacccaaagc caatacaatc ggcgggcacg cagcttgcgc
      361 ttactggtcg aagcctttcg gcggcattgg ctggacgaat tgggcttgct catgaaccaa
      421 gatttactct tggataccaa accagtgccc gtcatgggct acaaacgtag caaaaaacac
      481 agtgcttttg ccgggagtgc gggctatggg gtgtgcgtca gccggaacct gaagtattac
      541 ggctacaaac tggtcaccct cagcaccctg gaaggcatcc cgctggtcta cgagttagtg
      601 gcggctaatc tcgacgaacg ggtggcggca caagccgtgg tctattgtgt gcgtggctgt
      661 cgcatttatg gcgacaaagg gttccttggg gaagaatggc aacaccaact cttccagcac
      721 acccgaaatc gcttgttcac cgccaaacgg agcaatcaaa caacgcaaaa cccgagcgaa
      781 tttgaccgct ggctcaacag cctccgggaa cgcattgaag ggctattcaa cgaagtccag
      841 aacaccggtc gcaatttgga acgtttatta gcgaaaaccg tgttagggct aaccacacga
      901 gtgattgcga aaatgaccag tcatctcgtc aagctcattc ttcgaaagtg ctatgggatt
      961 gatgtgcaaa cctttacctg taccgcctga aatggtggcg cgaattcaca taagccgt
//
LOCUS       DAOQLC010000469         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_149189_length_1008_cov_19.377754, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000469 DAOQLC010000000
VERSION     DAOQLC010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149189_length_1008_cov_19.377754"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1008
                     /locus_tag="PL000_23520"
     CDS             <1..>1008
                     /locus_tag="PL000_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91903.1"
                     /translation="ILAISTLVRFDMAVPYIVIFAVLFFVQKENRKEHVIWGLGLLVL
                     SLGGQTLVRYLYYGALLPNTYYLKVEGWPLTLRLLRGLYALVHFAYYSNWVLFLLPFT
                     IFLFRRDWKVTLLALVFIAQVAYSVYVGGDAWENHGGANRYIVIAMPLFFVVFSWSAV
                     ELLKKAASNLFAQGGVLAAGDGGRSEHHEGNLFEIGWRVVFVILFTFSLLNFNALIGE
                     WKSIERWNFTRRPDYVAGSDRNLQIALAIENVTQPGASVAVIGAGTIPYLLPDRYAID
                     ILGKADPYIAHQNVRTPMSLADVPFMRPGHMKWDYAYTFGQLKPDVIVSIWEGTDAEA
                     AP"
BASE COUNT          192 a          285 c          270 g          261 t
ORIGIN      
        1 aattctagcc atttccaccc ttgtccgctt tgatatggcg gttccttaca ttgttatttt
       61 tgcggtgctg ttcttcgtgc aaaaagaaaa ccgcaaagag catgtgatct gggggctggg
      121 actgctcgtc ctctcgcttg gcgggcaaac cctggtgcgc tatctttatt acggcgccct
      181 gctcccaaac acctactacc tcaaagtgga aggctggccg ctcaccctgc gcctcctgcg
      241 cggactgtac gcgttggtcc attttgcata ttactcgaac tgggtgctct ttttactgcc
      301 gttcacgatc ttcctcttcc gccgcgattg gaaggtgact ttgctggcgt tggtcttcat
      361 cgcgcaggtg gcctattccg tgtacgtggg cggggatgca tgggagaatc acggcggggc
      421 gaatcgatac attgtcatcg ccatgccgtt gttttttgtt gtgttcagtt ggtcagcggt
      481 ggagttgttg aagaaggcag cttccaatct tttcgctcaa ggcggcgtcc tcgcagccgg
      541 ggacggcggg aggagcgaac atcacgaagg aaaccttttt gaaattggct ggcgggttgt
      601 atttgtcatc ctttttactt tttccctcct caacttcaat gcattgatcg gcgaatggaa
      661 atccatcgaa cgctggaatt tcactcggcg gcccgattac gtggcaggca gtgaccgcaa
      721 tttgcagatc gctctcgcca tcgaaaatgt gacccagccc ggggcgtccg tcgctgtcat
      781 cggcgcgggg acaattccct acctgctgcc agaccgctac gcgattgaca tcctcggcaa
      841 agccgacccg tacatcgcgc atcaaaacgt gcgcacaccg atgagccttg ccgatgtccc
      901 attcatgcgg cccggtcaca tgaaatggga ttacgcctat accttcggtc aactcaaacc
      961 tgatgtcatc gtctccattt gggaaggcac cgatgccgaa gccgcgcc
//
LOCUS       DAOQLC010000470         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate AMR_MDS_0558
            NODE_150118_length_1005_cov_28.073684, whole genome shotgun
            sequence.
ACCESSION   DAOQLC010000470 DAOQLC010000000
VERSION     DAOQLC010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810190
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (wastewater metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 05:53:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 3, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 6 (16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 3 of 23
            Pseudo Genes (incomplete)         :: 15 of 23
            Pseudo Genes (internal stop)      :: 8 of 23
            Pseudo Genes (multiple problems)  :: 3 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150118_length_1005_cov_28.073684"
                     /isolate="AMR_MDS_0558"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1005
                     /locus_tag="PL000_23525"
     CDS             <1..>1005
                     /locus_tag="PL000_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC91904.1"
                     /translation="GVALAKSQNYVIAFKEWSTEFFFDAGNATGSPLSPVDNGFSLIG
                     CASGESLAEVDGTLFWVSQTKQNGRGVHMMIGLESKDITTPSIQRILNRDTLSSLAAY
                     GLKIGGAVCYVLTLKNTGITVVYNVQSGKWSEWTSLTAGTPQSCTITRSGSVAIVVSA
                     GHGLADGDPVLIAGANDIEYNGTHQVNYIDADTFSFHVENSPVTPATGTITCTGYTSS
                     YFKLTKHVKALGKDILLHESNGHIYIPKDLHGKDDAPIDFVIRSGKFDGDSMQRKSCS
                     RTEVVGNKVDSEAMLRWSDDDYQSFSKYRRVDLSAEQSKLNRCGSFRRRAFELRHVAD
                     T"
BASE COUNT          244 a          254 c          265 g          242 t
ORIGIN      
        1 ggcgtagcac ttgccaagtc acaaaactat gtgattgcgt tcaaggaatg gagtacggaa
       61 ttcttctttg acgcaggcaa cgcaaccggc tccccgttgt ctccggtcga taacgggttc
      121 tcgcttattg ggtgcgcttc cggggagtct cttgcagaag tggatggaac gctgttctgg
      181 gtatctcaga caaagcagaa cgggcgcggc gttcatatga tgattggcct tgagtccaag
      241 gacattacga cgccaagcat tcaacgcatt ttgaatcgag acacgctttc gagtctcgcc
      301 gcttatggcc tgaagatcgg tggggctgtt tgttacgtcc tgacgctcaa aaacaccggc
      361 atcacggtcg tctataacgt ccaatccggc aagtggtctg aatggactag ccttaccgct
      421 ggcacccctc agtcatgcac gattaccaga agcggtagcg tggcaatagt cgttagcgca
      481 gggcatgggc tggccgatgg tgatccggtg cttatcgctg gtgcaaatga cattgaatac
      541 aacggaacgc atcaggtgaa ttacatcgac gccgacacgt tctctttcca tgtcgaaaat
      601 tccccggtca cgcctgcaac cggaacgatt acatgcaccg gctatacctc gtcttatttc
      661 aagctgacca agcacgttaa agcacttgga aaagacattc tcttgcacga atcgaacggg
      721 catatctaca tcccgaagga tctgcatgga aaggatgacg ccccgattga ttttgtcatc
      781 cggtcaggga agtttgatgg tgactcaatg cagaggaagt cttgctcacg caccgaagtc
      841 gttggcaaca aagtagatag cgaagccatg ctcagatggt cggatgatga ctatcaatcg
      901 ttttccaaat atcgccgcgt tgatctgtcg gcggaacaat caaaactgaa ccgttgcggt
      961 tctttccgtc gccgcgcttt tgaattgcgg catgtagcag atacc
//
